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Sample records for groundsel senecio vulgaris

  1. Final Critical Habitat for the San Francisco Peaks groundsel (Senecio franciscanus)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for San Francisco Peaks groundsel (Senecio franciscanus) occur based on the description...

  2. Final Critical Habitat for the San Francisco Peaks groundsel (Senecio franciscanus)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for San Francisco Peaks groundsel (Senecio franciscanus) occur based on the description...

  3. Effects of origin, seasons and storage under different temperatures on germination of Senecio vulgaris (Asteraceae) seeds

    Science.gov (United States)

    2016-01-01

    Invasive plants colonize new environments, become pests and cause biodiversity loss, economic loss and health damage. Senecio vulgaris L. (Common groundsel, Asteraceae), a widely distributing cosmopolitan weed in the temperate area, is reported with large populations in the north–eastern and south–western part, but not in southern, central, or north-western parts of China. We studied the germination behavior of S. vulgaris to explain the distribution and the biological invasion of this species in China. We used seeds originating from six native and six invasive populations to conduct germination experiments in a climate chamber and under outdoor condition. When incubated in a climate chamber (15 °C), seeds from the majority of the populations showed >90% germination percentage (GP) and the GP was equal for seeds with a native and invasive origin. The mean germination time (MGT) was significantly different among the populations. Under outdoor conditions, significant effects of origin, storage conditions (stored at 4 °C or ambient room temperature, ca. 27 °C) and seasons (in summer or autumn) were observed on the GP while the MGT was only affected by the season. In autumn, the GP (38.6%) was higher and the MGT was slightly longer than that in summer. In autumn, seeds stored at 4 °C showed higher GP than those stored at ambient room temperature (ca.27 °C), and seeds from invasive populations revealed higher GP than those from native populations. The results implied that the high temperature in summer has a negative impact on the germination and might cause viability loss or secondary dormancy to S. vulgaris seeds. Our study offers a clue to exploring what factor limits the distribution of S. vulgaris in China by explaining why, in the cities in South-East China and central China such as Wuhan, S. vulgaris cannot establish natural and viable populations. PMID:27602303

  4. Heteroplasmy and atrazine resistance in Chenopodium album and Senecio vulgaris.

    Science.gov (United States)

    Bühler, Michaela; Bogenrieder, Arno; Sandermann, Heinrich; Ernst, Dieter

    Atrazine-resistant weeds are well known, and the resistance is primarily caused by a point mutation in the psbA chloroplast gene encoding the photosystem II D1 protein. Heteroplasmy, the presence of different types of chloroplasts in an individual plant, is also very common. Thus, atrazine-resistant weeds may also partly possess the atrazine-binding sequence and vice versa. The region of the psbA gene containing the mutation was sequenced from atrazine-resistant and atrazine-sensitive Chenopodium album and Senecio vulgaris plants. In atrazine-sensitive C. album plants, the expected AGT triplet was found. The atrazine-resistant plants contained the expected base substitution (AGT to GGT); however, in addition the AGT triplet was found. The atrazine-resistant S. vulgaris plants contained the expected GGT sequence, whereas the atrazine-sensitive plants contained both the AGT and GGT sequences. This clearly indicates that in addition to Gly264 also Ser264 is present in atrazine-resistant plants, and vice versa in atrazine-sensitive plants, indicating heteroplasmy in these weeds.

  5. Dynamics of the weed infestation with Senecio vulgaris after a single entry from seeds

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    Söchting, Hans-Peter

    2014-02-01

    Full Text Available Due to a short generation time associated with a high seed production and a quick germination, which is possible throughout the year, Senecio vulgaris is especially in horticultural crops one of the most important weed species. Like all ragwort species, also Senecio vulgaris contains pyrrolizidine alkaloids which are converted in the liver to harmful substances. For this reason an accidental consumption of this species should be avoided completely. Belonging to the Compositae, chemical control of this weed species in vegetable crops, particularly in lettuce, is difficult. Based on a field-grown model experiment the emergence behavior and growth of Senecio vulgaris in leafy lettuce was studied. The first step was the contamination of the trial plots with Senecio seeds. For this purpose Senecio plants were planted at three different densities (1, 2 and 10 plants m2 in the designated plots. All plots were covered with fleece in order to prevent an unregulated dispersal of seeds. After seed maturity the fleece was removed, plants were cut into small pieces and the plant material including the seeds was incorporated into the soil. Then different leafy lettuces crops (rocket, asia green, spinach, lamb´s lettuce were cultivated in a six-crop sequence over two years (three crop sopecies per year. The development of Senecio vulgaris and the resulting possible contamination of the lettuces with Senecio leaves was recorded. From the date of removing the fleece on seed-production a shedding of Senecio plants was prevented to avoid further contamination. Also the entry from outside the plots was excluded. Depending on the initial plant density, the 71, 55 and 216 Senecio plants m2 which emerged after the first sowing of lettuce dropped to 7, 9 and 16 plants m2 after the sixth sowing. Thus, the density of S. vulgaris plants rapidly decreased but there was still a significant potential of emerging seedlings potentially contaminating the lettuce crops after

  6. Possibilities and limitations of sup 1 H and sup 13 C nuclear magnetic resonance spectroscopy for the identification and the quantitative determination of some naturally occurring carcinogenic risk factors. [Senecio vulgaris; Senecio vernalis; Senecio jacobaea; Euphorbia ingens

    Energy Technology Data Exchange (ETDEWEB)

    Pieters, L.

    1988-01-01

    The aim of this work was to develop a phytochemical screening method for some selected carcinogenic or tumor-promoting principles in higher plants. The pyrrolizidine alkaloids from some Senecio species (Compositae or Asteraceae), and the diterpene ester from Croton tiglium L. and Euphorbia ingens E. Mey (Euphorbiaceae) were chosen as representatives of both groups. The possibilities and limitations of {sup 1}H and {sup 13}C nuclear magnetic resonance spectroscopy ({sup 1}H and {sup 13}C NMR) for the analysis of mixtures of carcinogenic pyrrolizidine alkaloids were compared with high performance liquid chromatography, and gas chromatography with high performance liquid chromatography, and gas chromatography was well as gas chromatography - mass spectrometry. Senecio vulgaris L., Senecio vernalis Waldst. and Kit. and Senecio jacobaea L. were investigated.

  7. Eremophilane derivatives from Senecio dianthus.

    Science.gov (United States)

    Huang, Shuai; Zhou, Xian-li; Wang, Hong-yan; Wang, Cui-juan; Zhou, Xiao-li; Xiao, Feng; Weng, Jie

    2013-10-01

    From the aerial parts of Senecio dianthus, five eremophilane glucosides (1, 2, 4-6) and one new eremophilenolide (7) were isolated, together with sixteen known compounds (3, 8-22). Their structures and relative configurations were elucidated on the basis of extensive spectroscopic analysis, including HR-ESI-MS, X-ray, CD, 1D- and 2D-NMR experiments.

  8. Hybrid swarms : catalysts for multiple evolutionary events in Senecio in the British Isles

    OpenAIRE

    Lowe, A. J.; Abbott, R J

    2015-01-01

    Background: Introgressive hybridisation is an evolutionary catalyst producing novel variants able to explore new ecological niches and evolve as new hybrid taxa. However, the role of ‘hybrid swarms’ – highly variable populations produced following interspecific hybridisation – in generating this evolutionary novelty has been poorly studied. Aims: We examine the alternative origins of tetraploid hybrid derivatives of Senecio vulgaris and S. squalidus, via local polytopic formation or long-dist...

  9. Vulgaris

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    Mukaddes Kavala

    2012-01-01

    Full Text Available Background. Thyroid disorders may affect all of the organ systems of the body and they are also highly associated with a wide variety of skin disorders. The aim of this study was to investigate the prevalence of thyroid function abnormalities and thyroid autoimmunity in patients with pemphigus vulgaris (PV and to determine the association between thyroid disorders and clinical involvement and systemic corticosteroid treatment in patients with PV. Methods. The study consisted of eighty patients with PV and eighty healthy individuals. Thyroid functions (fT3, fT4, and TSH and thyroid autoimmunity (anti-thyroid peroxidase (anti-TPO, and anti-thyroglobulin (anti-Tg antibodies were investigated in both groups. Primary thyroid disease (PTD was diagnosed with one or more of the following diagnostic criteria: (i positive antithyroid antibodies, (ii primary thyroid function abnormalities. Results. Significant changes in the serum thyroid profile were found in 16% (13/80 of the PV group and 5% (4/80 of the control group. Positive titers of antithyroid antibodies (anti-TPO and anti-Tg were observed in 7 patients (9% with PV and one in the control group (1,2%. Hashimoto thyroiditis was diagnosed in 9% of PV patients and it was found to be more prevalent in the mucosal form of PV. PTD was found in 13 of (%16 PV patients which was significantly high compared to controls. PTD was not found to be associated with systemic corticosteroid use. Free T3 levels were significantly lower in PV group compared to the control group and free T4 levels were significantly higher in PV group compared to the controls. Conclusions. PV may exist together with autoimmune thyroid diseases especially Hashimoto thyroiditis and primer thyroid diseases. Laboratory work-up for thyroid function tests and thyroid autoantibodies should be performed to determine underlying thyroid diseases in patients with PV.

  10. Pyrrolizidine alkaloids of senecio sp from Peru

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    Liliana Ruiz Vásquez and Matías Reina Artiles

    2011-01-01

    Full Text Available Six pyrrolizidine alkaloids (PAs (two saturated macrocyclic, three unsaturated macrocyclic and one unsaturated seco-macrocyclic were isolated from native Peruvian Senecio species. The structures of these alkaloids were established by a complete NMR spectroscopic analysis, chemical transformations and comparison of their NMR data with those published for similar alkaloids. Three PAs were then tested for antifungal activity against Fusarium moniliforme, F. (Sheldon, F. oxysporum fs. lycopersici (Scheldt and F. solani (Mart, no significant activity being observed.

  11. Pyrrolizidine alkaloids of senecio sp from Peru

    OpenAIRE

    2011-01-01

    Six pyrrolizidine alkaloids (PAs) (two saturated macrocyclic, three unsaturated macrocyclic and one unsaturated seco-macrocyclic) were isolated from native Peruvian Senecio species. The structures of these alkaloids were established by a complete NMR spectroscopic analysis, chemical transformations and comparison of their NMR data with those published for similar alkaloids. Three PAs were then tested for antifungal activity against Fusarium moniliforme, F. (Sheldon), F. oxysporum fs. lycopers...

  12. Pyrrolizidine alkaloids of Senecio sp from Peru

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz Vasquez, Liliana; Reina Artiles, Matias [Instituto de Productos Naturales y Agrobiologia, CSIC, Tenerife (Spain); Gonzalez Coloma, Azucena [Instituto de Ciencias Agrarias (ICA), CSIC, Madrid (Spain); Cabrera Perez, Raimundo [Universidad de La Laguna (ULL), Tenerife (Spain). Unidad de Fitopatologia, Facultad de Biologia; Ruiz Mesia, Lastenia [Universidad Nacional de la Amazonia Peruana (LIPNAA-UNAP), AA.HH. Nuevo San Lorenzo, San Juan, Iquitos (Peru). Lab. de Investigacion en Productos Naturales Antiparasitarios de la Amazonia

    2011-07-01

    Six pyrrolizidine alkaloids (PAs) (two saturated macrocyclic, three unsaturated macrocyclic and one unsaturated seco-macrocyclic) were isolated from native Peruvian Senecio species. The structures of these alkaloids were established by a complete NMR spectroscopic analysis, chemical transformations and comparison of their NMR data with those published for similar alkaloids. Three PAs were then tested for antifungal activity against Fusarium moniliforme, F. (Sheldon), F. oxysporum fs. lycopersici (Scheldt) and F. solani (Mart), no significant activity being observed. (author)

  13. Three new caespitose species of Senecio (Asteraceae, Senecioneae) from South Peru.

    Science.gov (United States)

    Tubée, Daniel B Montesinos

    2014-01-01

    Three new species of the genus Senecio (Asteraceae, Senecioneae) belonging to Senecio ser. Suffruticosi subser. Caespitosi were discovered in the tributaries of the upper Tambo River, Moquegua Department, South Peru. Descriptions, diagnoses and discussions about their distribution, a table with the morphological similarities with other species of Senecio, a distribution map, conservation status assessments, and a key to the caespitose Peruvian species of Senecio subser. Caespitosi are provided. The new species are Senecio moqueguensis Montesinos, sp. nov. (Critically Endangered) which most closely resembles Senecio pucapampaensis Beltrán, Senecio sykorae Montesinos, sp. nov. (Critically Endangered) which most closely resembles Senecio gamolepis Cabrera, and Senecio tassaensis Montesinos, sp. nov. (Critically Endangered) which most closely resembles Senecio moqueguensis Montesinos.

  14. A New Bezoxepine Derivative from Senecio desfontainei

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    Syed Hamid Hussain

    2013-08-01

    Full Text Available Phytochemical investigation of the aerial parts of Senecio desfontainei afforded one new benzoxepin derivative, Senecioside (1 along with eleven known compounds (2-12. Their structures were assigned from 1H and 13C NMR spectra, DEPT and by 2D COSY, NOE, HSQC and HMBC experiments. All these compounds were tested for antioxidant activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH radical scavenging assay. Among them, compounds 2, 3, 4, 6 and 9 showed significant antioxidant activity against DPPH radicals.

  15. [A new eremophilane derivative from Senecio dianthus].

    Science.gov (United States)

    Han, He-Dong; Hu, Hai-Qing; Li, Yan; Wang, Xiao-Ling

    2013-10-01

    A new eremophilane derivative, 4,5,11-trimethyl-9( 10), 7 ( 11) -eremophiladien-8-keto-12-carboxylic acid-beta-D-glucopyranoside( which named dianthuside A) 1 and four known compounds, 5,7,4'-trihydroxy-flavonone-3-0-beta-D-glucoside (2), quercetin-3-0-beta-D-glucoside(3) ,hyperin(4) and rutin(5) have been isolated from the aerial part of Senecio dianthus. Their structures were elucidated by physicochemical properties and spectroscopic data analysis. Compounds 2, 4 and 5 were isolated from this plant for the first time.

  16. Three new caespitose species of Senecio (Asteraceae, Senecioneae from South Peru

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    Daniel Montesinos Tubée

    2014-06-01

    Full Text Available Three new species of the genus Senecio (Asteraceae, Senecioneae belonging to S. ser. Suffruticosi subser. Caespitosi were discovered in the tributaries of the upper Tambo River, Moquegua Department, South Peru. Descriptions, diagnoses and discussions about their distribution, a table with the morphological similarities with other species of Senecio, a distribution map, conservation status assessments, and a key to the caespitose Peruvian species of S. subser. Caespitosi are provided. The new species are Senecio moqueguensis Montesinos, sp. nov. (Critically Endangered which most closely resembles Senecio pucapampaensis Beltrán, Senecio sykorae Montesinos, sp. nov. (Critically Endangered which most closely resembles Senecio gamolepis Cabrera, and Senecio tassaensis Montesinos, sp. nov. (Critically Endangered which most closely resembles Senecio moqueguensis Montesinos.

  17. The toxicity of Senecio inaequidens DC.

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    A.F.P. Dimande

    2007-06-01

    Full Text Available This study was designed to confirm the toxicity of a plant implicated in an outbreak of poisoning of stock in Frankfort, Free State Province, South Africa. Cows died acutely after being introduced into a camp, where an abundant, green shrublet was noted to be heavily grazed. This plant was subsequently identified as Senecio inaequidens DC. (Asteraceae by the South African National Biodiversity Institute (SANBI. Extraction and chemical analyses for pyrrolizidine alkaloids (PAs in Senecio inaequidens revealed the presence of 4 different compounds, namely retrorsine and senecionine (known to be hepatotoxic and 2 unidentified compounds. The average total PA (free base plus N-oxide concentration in plant parts of S. inaequidens collected at Frankfort during the outbreak was 0.81 %, compared with the total alkaloid content in the dried, milled S. inaequidens plant material, collected 7 weeks after the outbreak, of only 0.18 %. Male Sprague-Dawley rats (n=4, aged 8-9 weeks, were dosed per os. Each rat received a different dose of the crude Senecio inaequidens extract, ranging from 0.049 mg/g body weight (b.w. to 0.25 mg/g b.w. No clinical signs were observed in the rat receiving the lowest dose. Rats receiving higher doses showed depression, an unsteady gait, pilo-erection and jaundice, which was particularly noticeable in the ears. Clinical chemistry evaluation revealed an increase in the activities of ALP (except Rat 4, AST and GGT in all animals. Total serum bilirubin, creatinine and urea concentrations were also elevated. All rats had low serum globulin concentrations with an A/G ratio above 1.2. Post mortem examination of the rats revealed marked hepatic lesions. Histopathologically, these changes were characterised by necrosis (variable in extent of the centrilobular and midzonal hepatocytes (but sparing the portal hepatocytes, with extensive haemorrhage and congestion. Proliferation of the bile ducts, fibrosis and oedema were also present

  18. Consideraciones taxonómicas en especies de Senecio de Argentina Taxonomic considerations on Argentine species of Senecio

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    Roberto D. Tortosa

    2010-12-01

    Full Text Available Se incluyen algunas especies de Senecio de Argentina, reconocidas hasta el presente como especies válidas, en la sinonimia de otras: S. francisci Phil. en S. polygaloides Phil.; S. gymnocaulos Phil. en S. kingii Hook. f.; S. molinae Phil. en S. glaber Less.; S. mustersii Speg. y su var. dentatus Cabrera en S. subumbellatus Phil. Senecio microcephalus Phil. y su var. angustifolius Cabrera son consideradas una variedad de S. subumbellatus y S. schreiteri Cabrera una variedad de S. leucostachys Baker. No se acepta la validez de las variedades Senecio gilliesii Hook. et Arn. var. dasycarpus Cabrera, S. glaber Less. var. pratensis (Phil. Cabrera, S. gnidioides Phil. var. gilvus (Phil. Cabrera, S. kingii Hook. f. var. paradoxus (Albov ex Kurtz Cabrera, S. leucomallus A. Gray var. incisus A. Gray, S. linariifolius Poepp. ex DC. var. heliophytoides (Phil. Reiche y var. subtomentosus Cabrera y de la forma S. perezii Cabrera fo. integerrimus Cabrera.

  19. Pyrrolizidine alkaloids from Bulgarian species of the genus Senecio

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    NADEZHDA KOSTOVA

    2006-12-01

    Full Text Available Nine Bulgarian species from the genus Senecio were studied phytochemically and/or by GC-MS analysis. Senecivernine-N-oxide was isolated and identified by spectral data for the first time. Different types of pyrrolizidine alkaloids were tested for cytotoxicity on murine lymphocytes. At a concentration of 100 µg/ml, the alkaloid retroisosenine showed immunosuppressive effect.

  20. 千里光提取物体外抗阴道毛滴虫的效果观察%The effects of the extracts of groundsel on the Trichomonas vaginalis in vitro

    Institute of Scientific and Technical Information of China (English)

    张静; 叶彬; 武卫华; 邹晓毅

    2011-01-01

    目的 研究千里光提取物对体外培养的阴道毛滴虫的杀虫效果.方法 体外培养阴道毛滴虫,选择千里光提取物进行体外抗阴道毛滴虫实验,千里光药液浓度经稀释后分别为4,2,1,0.5,0.25 mg/ml.同时以甲硝唑为阳性对照,乙醇及RPMI-1640为阴性对照.结果 体外实验表明千里光在24 h时杀灭阴道毛滴虫的最低有效浓度为2 mg/ml.其杀虫效果与甲硝唑近似;阴道毛滴虫在RPMI-1640培养液中生长良好,增值3倍.结论 千里光提取物在体外具有杀灭和抑制阴道毛滴虫生长的作用.%Objective To study the effects of Climbing Groundsel Herb on Trichomonas voginalis in vitro. Methods Trichomonas vaginalis was treated with 4,2,1,0.5,0.25mg/ml of Climbing Groundsel Herb extract in vitro. Metronidazole was chosen to act as positive control and RPMI-1640 and 1% ethanol was chosen to act as negative control. Result The results of trialed medicines on the Trichornonas vaginalis in vitro showed that minimal lethal concentrations (MLCs) of extracts of Climbing Groundsel Herb on the Trichomonas vaginalis was 2 mg/ml. Conclusion Climbing Groundsel Herb was demonstrated the effects against Trichomonas vaginalis in vitro.

  1. Bioactive furanoeremophilanes from Senecio otites Kunze ex DC.

    Science.gov (United States)

    Domínguez, Dulce M; Reina, Matías; Villarroel, Luis; Fajardo, Victor; González-Coloma, Azucena

    2008-01-01

    The furanoeremophilanes 6 beta-angeloyloxy-1,10-dehydrofuranoeremophilan-9-one (1), 6 beta-hydroxy-1,10-dehydrofuranoeremophilan-9-one (2) and 6 beta-propionyloxy-1,10-dehydrofuranoeremophilan-9-one (3) were isolated from Senecio otites, their structures elucidated by spectral analyses, and their insecticidal and phytotoxic properties evaluated. Compounds 1-3 proved to be effective aphid antifeedants against Myzus persicae and Rhopalosiphum padi and had postingestive negative effects on Spodoptera littoralis larvae. These compounds did not have any phytotoxic effects on Lactuca sativa.

  2. Othonna sonchifolia L. is an earlier name for Senecio cymbalarifolius L. (Asteraceae: Senecioneae

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    Luvo Magoswana

    2017-02-01

    Full Text Available Background: Ongoing systematic studies in the African flora necessitate periodic nomenclatural adjustments and corrections.Objectives: To effect requisite nomenclatural changes.Method: Relevant literature was surveyed and type material located and examined.Results: A nomenclatural correction is necessary in Othonna L. and Senecio L. (Asteraceae.Conclusions: Othonna sonchifolia L. is recognised as the earliest name for Senecio cymbalarifolius (L. Less. and the new combination Senecio sonchifolius (L. J.C. Manning & Magoswana is provided for the species. Othonna cymbalarifolia is lectotypified and Senecio hastifolius (L. Less. is reinstated from synonymy as a species distinct from S. sonchifolius.

  3. New records of pyrrolizidine alkaloid-feeding insects. Hemiptera and Coleoptera on Senecio brasiliensis.

    Science.gov (United States)

    Klitzke; Trigo

    2000-04-01

    New records are reported for a Hemipteran (Largus rufipennis) and a Coleopteran (Chauliognathus fallax) feeding on Senecio brasiliensis (Asteraceae) and sequestering pyrrolizidine alkaloids (PAs). Both insects are warningly colored and rejected by predators.

  4. Pyrrolizidine alkaloids from seven wild-growing Senecio species in Serbia and Montenegro

    OpenAIRE

    2009-01-01

    The genus Senecio (family Asteraceae) is one of the largest in the world. It comprises about 1100 species which are the rich source of pyrrolizidine alkaloids. Plants containing pyrrolizidine alkaloids are among the most important sources of human and animal exposure to plant toxins and carcinogens. The pyrrolizidine alkaloids of seven Senecio species (S. erucifolius, S. othonnae, S. wagneri, S. subalpinus, S. carpathicus, S. paludosus and S. rupestris) were studied. Fourteen alkaloids were i...

  5. Alcalóides pirrolizidínicos em espécies do gênero Senecio

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    Silva Chana de Medeiros da

    2006-01-01

    Full Text Available Senecio species contain a large variety of secondary metabolites and many of these plants afford pyrrolizidine alkaloids. This paper is a review of the literature, describing 62 pyrrolizidine alkaloids already isolated in 62 of more than 2000 species of Senecio, distributed worldwide. The structure-activity relationships involving their toxicity are also discussed, since some Senecio species used for medicinal purposes are responsible for causing serious adverse effects.

  6. Alcalóides pirrolizidínicos em espécies do gênero Senecio

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    Chana de Medeiros da Silva

    2006-10-01

    Full Text Available Senecio species contain a large variety of secondary metabolites and many of these plants afford pyrrolizidine alkaloids. This paper is a review of the literature, describing 62 pyrrolizidine alkaloids already isolated in 62 of more than 2000 species of Senecio, distributed worldwide. The structure-activity relationships involving their toxicity are also discussed, since some Senecio species used for medicinal purposes are responsible for causing serious adverse effects.

  7. Ichthyosis vulgaris

    DEFF Research Database (Denmark)

    Thyssen, J P; Godoy-Gijon, E; Elias, P M

    2013-01-01

    Ichthyosis vulgaris is caused by loss-of-function mutations in the filaggrin gene (FLG) and is characterized clinically by xerosis, scaling, keratosis pilaris, palmar and plantar hyperlinearity, and a strong association with atopic disorders. According to the published studies presented...... in this review article, FLG mutations are observed in approximately 7·7% of Europeans and 3·0% of Asians, but appear to be infrequent in darker-skinned populations. This clinical review article provides an overview of ichthyosis vulgaris epidemiology, related disorders and pathomechanisms. Not only does...... ichthyosis vulgaris possess a wide clinical spectrum, recent studies suggest that carriers of FLG mutations may have a generally altered risk of developing common diseases, even beyond atopic disorders. Mechanistic studies have shown increased penetration of allergens and chemicals in filaggrin...

  8. Pemphigus vulgaris

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    Sandhya Tamgadge

    2011-01-01

    Full Text Available Pemphigus vulgaris is a chronic autoimmune mucocutaneous disease that initially manifests in the form of intraoral lesions, which spread to other mucous membranes and the skin. The etiology of pemphigus vulgaris is still unknown, although the disease has attracted considerable interest. The pemphigus group of disease is characterized by the production of autoantibodies against intercellular substances and is thus classified as autoimmune diseases. Most patients are initially misdiagnosed and improperly treated for months or even years. Dental professionals must be sufficiently familiar with the clinical manifestations of pemphigus vulgaris to ensure early diagnosis and treatment, since this in turn determines the prognosis and course of the disease. This article presents a case report with unknown etiology along with an overview of the disease.

  9. Acne vulgaris

    OpenAIRE

    Purdy, Sarah; DeBerker, David

    2008-01-01

    Acne vulgaris affects over 80% of teenagers, and persists beyond the age of 25 years in 3% of men and 12% of women. Typical lesions of acne include comedones, inflammatory papules, and pustules. Nodules and cysts occur in more severe acne, and can cause scarring and psychological distress.

  10. Acne vulgaris

    OpenAIRE

    Purdy, Sarah; de Berker, David

    2011-01-01

    Acne vulgaris affects over 80% of teenagers, and persists beyond the age of 25 years in 3% of men and 12% of women. Typical lesions of acne include comedones, inflammatory papules, and pustules. Nodules and cysts occur in more severe acne, and can cause scarring and psychological distress.

  11. Senecio rosmarinus y S. spegazzinii: Sinónimos de S. volckmannii (Asteraceae, Senecioneae Senecio rosmarinus and S. spegazzinii synonyms of S. volckmannii (Asteraceae, Senecioneae

    Directory of Open Access Journals (Sweden)

    Roberto D. Tortosa

    2006-07-01

    Full Text Available Se incluyen a Senecio rosmarinus Phil. y a S. spegazzinii Cabrera en la sinonimia de S. volckmannii Phil.(sección Suffrutecius Cabrera subsección Candidi Cabrera, especie con capítulos discoides, normalmente solitarios en los extremos de los tallos, con involucro anchamente acampanado y con 15-24 filarios. El indumento de la planta, la forma de las hojas y la pubescencia de los aquenios son caracteres variables dentro de la especie.Senecio rosmarinus Phil. and S. spegazzinii Cabrera are included as synonyms of S. volckmannii Phil. (section Suffrutecius Cabrera subsection Candidi Cabrera, species with discoid heads generally solitary at the end of stems, involucre broadly campanulate, with 15-24 phyllaries. The hair covering of plants, the shape of leaves and the pubescence of achenes are variable within this species.

  12. Three new caespitose species of Senecio (Asteraceae, Senecioneae) from South Peru

    NARCIS (Netherlands)

    Montesinos, D.B.

    2014-01-01

    Three new species of the genus Senecio (Asteraceae, Senecioneae) belonging to S. ser. Suffruticosi subser. Caespitosi were discovered in the tributaries of the upper Tambo River, Moquegua Department, South Peru. Descriptions, diagnoses and discussions about their distribution, a table with the morph

  13. Carbon isotope fractionation during photorespiration and carboxylation in Senecio.

    Science.gov (United States)

    Lanigan, Gary J; Betson, Nicholas; Griffiths, Howard; Seibt, Ulli

    2008-12-01

    The magnitude of fractionation during photorespiration and the effect on net photosynthetic (13)C discrimination (Delta) were investigated for three Senecio species, S. squalidus, S. cineraria, and S. greyii. We determined the contributions of different processes during photosynthesis to Delta by comparing observations (Delta(obs)) with discrimination predicted from gas-exchange measurements (Delta(pred)). Photorespiration rates were manipulated by altering the O(2) partial pressure (pO(2)) in the air surrounding the leaves. Contributions from (13)C-depleted photorespiratory CO(2) were largest at high pO(2). The parameters for photorespiratory fractionation (f), net fractionation during carboxylation by Rubisco and phosphoenolpyruvate carboxylase (b), and mesophyll conductance (g(i)) were determined simultaneously for all measurements. Instead of using Delta(obs) data to obtain g(i) and f successively, which requires that b is known, we treated b, f, and g(i) as unknowns. We propose this as an alternative approach to analyze measurements under field conditions when b and g(i) are not known or cannot be determined in separate experiments. Good agreement between modeled and observed Delta was achieved with f = 11.6 per thousand +/- 1.5 per thousand, b = 26.0 per thousand +/- 0.3 per thousand, and g(i) of 0.27 +/- 0.01, 0.25 +/- 0.01, and 0.22 +/- 0.01 mol m(-2) s(-1) for S. squalidus, S. cineraria, and S. greyii, respectively. We estimate that photorespiratory fractionation decreases Delta by about 1.2 per thousand on average under field conditions. In addition, diurnal changes in Delta are likely to reflect variations in photorespiration even at the canopy level. Our results emphasize that the effects of photorespiration must be taken into account when partitioning net CO(2) exchange of ecosystems into gross fluxes of photosynthesis and respiration.

  14. Silaum silaus- und Senecio aquaticus-Wiesen in Hessen : ein Beitrag zu ihrer Systematik, Verbreitung und Ökologie

    OpenAIRE

    Bergmeier, Erwin; Nowak, Bernd; Wedra, Christel

    1984-01-01

    Aus Hessen werden Wiesen mit Silaum silaus und Senecio aquaticus beschrieben und durch Vegetationsaufnahmen belegt. Unter Auswertung der Literatur wird die synsystematische Stellung der Gesellschaften diskutiert und ihre Zuordnung zum Sanguisorbo-Silaetum silai (Klapp 1951) Vollrath 1965 (Molinion) und Senecioni-Brometum racemosi Tx. et Prsg. 1951 ex auct. (Calthion) vorgeschlagen. Meadows with Silaum silaus and Senecio aquaticus have been described in Hessen and documented by phytosociolo...

  15. Senecio brasiliensis and pyrrolizidine alkaloids: toxicity to animals and human health

    Directory of Open Access Journals (Sweden)

    Helenice de Souza Spinosa

    2013-06-01

    Full Text Available Most economic losses in Brazilian livestock production, especially in horses and cattle, are due to poison plants, such as those of the genus Senecio. Senecio brasiliensis Lessing is the main cause of death in cattle in the state of Rio Grande do Sul. The toxicity of this genus is attributed to their content of pyrrolizidine alkaloids, which undergo liver biotransformation creating toxic metabolites, namely pyrroles. These compounds can be transferred to humans through contaminated animal products or by the use of this plant in folk medicine. Thus, the present article is a review of the species S. brasiliensis, its toxic active principles and the mechanism by which pathogenesis occurs. Other plants with the same toxic principles that are harmful to human health are covered as well.

  16. Pyrrolizidine alkaloids from seven wild-growing Senecio species in Serbia and Montenegro

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    BORIS M. MANDIC

    2009-01-01

    Full Text Available The genus Senecio (family Asteraceae is one of the largest in the world. It comprises about 1100 species which are the rich source of pyrrolizidine alkaloids. Plants containing pyrrolizidine alkaloids are among the most important sources of human and animal exposure to plant toxins and carcinogens. The pyrrolizidine alkaloids of seven Senecio species (S. erucifolius, S. othonnae, S. wagneri, S. subalpinus, S. carpathicus, S. paludosus and S. rupestris were studied. Fourteen alkaloids were isolated and their structures determined from spectroscopic data (1H- and 13C-NMR, IR and MS. Five of them were identified in S. erucifolius, four in S. othonnae, two in S. wagneri, four in S. subalpinus, two in S. carpathicus, three in S. paludosus and three in S. rupestris. Seven pyrrolizidine alkaloids were found for the first time in particular species. The results have chemotaxonomic importance. The cytotoxic activity and antimicrobial activity of some alkaloids were also studied.

  17. Acne vulgaris.

    Science.gov (United States)

    Moradi Tuchayi, Sara; Makrantonaki, Evgenia; Ganceviciene, Ruta; Dessinioti, Clio; Feldman, Steven R; Zouboulis, Christos C

    2015-09-17

    Acne vulgaris is a chronic inflammatory disease - rather than a natural part of the life cycle as colloquially viewed - of the pilosebaceous unit (comprising the hair follicle, hair shaft and sebaceous gland) and is among the most common dermatological conditions worldwide. Some of the key mechanisms involved in the development of acne include disturbed sebaceous gland activity associated with hyperseborrhoea (that is, increased sebum production) and alterations in sebum fatty acid composition, dysregulation of the hormone microenvironment, interaction with neuropeptides, follicular hyperkeratinization, induction of inflammation and dysfunction of the innate and adaptive immunity. Grading of acne involves lesion counting and photographic methods. However, there is a lack of consensus on the exact grading criteria, which hampers the conduction and comparison of randomized controlled clinical trials evaluating treatments. Prevention of acne relies on the successful management of modifiable risk factors, such as underlying systemic diseases and lifestyle factors. Several treatments are available, but guidelines suffer from a lack of data to make evidence-based recommendations. In addition, the complex combination treatment regimens required to target different aspects of acne pathophysiology lead to poor adherence, which undermines treatment success. Acne commonly causes scarring and reduces the quality of life of patients. New treatment options with a shift towards targeting the early processes involved in acne development instead of suppressing the effects of end products will enhance our ability to improve the outcomes for patients with acne.

  18. Sesquiterpenes, flavonoids, shikimic acid derivatives and pyrrolizidine alkaloids from Senecio kingii Hook.

    Science.gov (United States)

    Ruiz-Vásquez, Liliana; Reina, Matías; López-Rodríguez, M; Giménez, Cristina; Cabrera, Raimundo; Cuadra, Pedro; Fajardo, Víctor; González-Coloma, Azucena

    2015-09-01

    Twenty-four compounds including eleven eremophilanolides (1-11), one eremophilane (13), five shikimic acid derivatives (14-18), six flavonoids (19-24), and the macrocyclic unsaturated pyrrolizidine alkaloid integerrimine (25) were isolated from Senecio kingii, an endemic species from the Magallanes Region (Chile). Compounds 3, 5, 6, 8-11 and 13-18 have not been previously reported as natural products. Their molecular structures were determined by NMR spectroscopic analysis and comparison with published NMR data. An X-ray-analysis of compound 3 has been performed. Their insecticidal and antifungal activities were tested, being compound 3 the strongest insect antifeedant. Compounds 6, 9 and 18 were moderate antifungals.

  19. Analysis of pyrrolizidine alkaloids and evaluation of some biological activities of Algerian Senecio delphinifolius (Asteraceae).

    Science.gov (United States)

    Tidjani, Soukaina; Okusa, Philippe N; Zellagui, Amar; Banuls, Laetitia Moreno Y; Stévigny, Caroline; Duez, Pierre; Rhouati, Salah

    2013-04-01

    Although Senecio species are known as sources of potentially toxic pyrrolizidine alkaloids (PAs), some species of this genus are traditionally used as remedies, notably in Algeria. In this paper, the evaluation of biological activities and the analysis of PAs of Algerian specimens of Senecio delphinifolius Vahl are reported. The n-butanolic extract of the herb showed a weak antibacterial effect against Escherichia coli with a MIC of 1 mg/mL, but was inactive against Staphylococcus aureus and Pseudomonas aeruginosa. The n-butanolic extracts of the roots, stems and herb showed a modest antioxidant activity, scavenging the free radical DPPH with respective IC50 values of 55.3, 50.2 and 13.3 microg/mL. A cytotoxic effect against a series of human tumor cell lines was observed with the n-butanolic extract from stems (IC50 ranging between 34 and 88 microg/mL). The herb of the evaluated sample contains 140 ppm of PAs (senecionine, seneciphylline, integerrimine, senkirkine) and PA-related alkaloids (dehydrosenkirkine and neosenkirkine). As the major PAs belong to the toxic series (1,2-unsaturation in the pyrrolizidine cycle and macrocyclic diester), the use of S. delphinifolius should be discouraged in traditional medicine.

  20. Clinical and pathological aspects of chronic Senecio spp. poisoning in sheep

    Directory of Open Access Journals (Sweden)

    Paula R. Giaretta

    2014-10-01

    Full Text Available This paper describes an outbreak of chronic Senecio spp. poisoning in grazing sheep in Rio Grande do Sul, Brazil, causing the death of 10 out of 860 adult sheep. Eight sick ewes were euthanized and necropsied. Cattle from this farm were also affected. Clinical signs included progressive weight loss, apathy and photosensitization. Four out of seven tested sheep had increased gamma-glutamyl transferase serum activity and two of them presented serum elevation of alkaline phosphatase. At necropsy, three out of eight ewes presented slightly irregular toughened livers with multifocal nodules, two out of eight ewes had a whitish liver with thickened fibrotic Glisson's capsule partially adhered to the diaphragm, and three out of eight ewes had smooth and grossly normal livers. Necropsy findings attributed to liver failure included hydropericardium (7/8, ascites (5/8, icterus (2/8, hydrothorax (1/8, and edema of mesentery (1/8. The main hepatic histological findings that allowed the establishment of the diagnosis were megalocytosis, proliferation of bile ducts and fibrosis. Spongy degeneration was observed in the brains of all eight necropsied sheep and was more severe at the cerebellar peduncles, mesencephalon, thalamus, and pons. These are suggested as the portions of election to investigate microscopic lesions of hepatic encephalopathy in sheep with chronic seneciosis. The diagnosis of Senecio spp. poisoning was based on epidemiology, clinical signs, laboratory data, necropsy and histological findings.

  1. Nova nalazišta vrste Senecio inaequidens DC. u Hrvatskoj

    OpenAIRE

    Milović, Milenko; Pandža, Marija

    2014-01-01

    Senecio inaequidens DC. (Asteraceae) je neofit podrijetlom iz Južne Afrike koji je u Europu slučajno unesen uvozom vune krajem 19. i početkom 20. stoljeća. Od 70-tih godina prošlog stoljeća brzo se proširio većim dijelom srednje, zapadne i južne Europe. Nakon nedavno objavljenog nalaza u Hrvatskom zagorju (Sv. Križ Začretje), u ovom radu se navode dva nova nalazišta u Dalmaciji (Dicmo i Biograd). Zbog odgovarajuće klime i velikog broja pogodnih staništa, možemo očekivati udomaćenje i širenje ...

  2. Phenotypic traits variation among native diploid, native tetraploid and invasive tetraploid Senecio inaequidens DC. (Asteraceae

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    Monty, A.

    2010-01-01

    Full Text Available Senecio inaequidens DC. is a rapidly spreading plant invader in Europe. In its native range, it occurs at two co-existing diploid and tetraploid cytotypes. To date, only tetraploids are reported in Europe, even though invasive diploids were recorded in other parts of the world. We compared native diploid and both native and invasive tetraploid populations in common gardens in Europe for a suite of life history traits. Diploids were able to develop, showed high biomass production and produced more flower heads than tetraploids. In contrast, winter survival was null for diploids. It was low for native tetraploids, but reached 40% in invasive tetraploids. Results suggested that diploid cytotype tends to an annual life form when grown in Western Europe, with earlier and more abundant flowering. In contrast, the tetraploid cytotype was mainly perennial which may enhance its invasiveness.

  3. Defensive sesquiterpenes from Senecio candidans and S. magellanicus, and their structure-activity relationships.

    Science.gov (United States)

    Reina, Matías; Santana, Omar; Domínguez, Dulce M; Villarroel, Luis; Fajardo, Víctor; Rodríguez, Matías L; González-Coloma, Azucena

    2012-03-01

    Eleven eremophilanolides, 1-3 and 6-13, and two eremophilanes, 24 and 25, were isolated from Senecio candidans and S. magellanicus from the Magallanes Region (Chile). Compounds 2, 3, 9, and 10 have not been previously reported as natural products. Their structures were established by NMR spectroscopic analysis and chemical transformations. The X-ray analysis of compounds 11, 13, and 17 were also performed. Different semisynthetic analogs from eremophilanolide 11 were generated to carry out a structure-activity relationship study. Their possible plant defensive role was tested against herbivorous insects (Spodoptera littoralis, Rhopalosiphum padi, and Myzus persicae) and plants (Lactuca sativa). Additionally, their effects on insect (Sf9) and mammalian (CHO) cell lines were tested. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich.

  4. Complete 1H NMR assignments of pyrrolizidine alkaloids and a new eudesmanoid from Senecio polypodioides.

    Science.gov (United States)

    Villanueva-Cañongo, Claudia; Pérez-Hernández, Nury; Hernández-Carlos, Beatriz; Cedillo-Portugal, Ernestina; Joseph-Nathan, Pedro; Burgueño-Tapia, Eleuterio

    2014-05-01

    Chemical investigation of the aerial parts of Senecio polypodioides lead to the isolation of the new eudesmanoid 1β-angeloyloxyeudesm-7-ene-4β,9α-diol (1) and the known dirhamnosyl flavonoid lespidin (3), while from roots, the known 7β-angeloyloxy-1-methylene-8α-pyrrolizidine (5) and sarracine N-oxide (6), as well as the new neosarracine N-oxide (8), were obtained. The structure of 1 and 8 was elucidated by spectral means. Complete assignments of the (1)H NMR data for 5, 6, sarracine (7), and 8 were made using one-dimensional and two-dimensional NMR experiments and by application of the iterative full spin analysis of the PERCH NMR software.

  5. Morphometry and Distribution of Senecio Nemorensis agg. Species (Asteraceae in Poland

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    Rola Kaja

    2014-07-01

    Full Text Available A morphometric analysis based on 316 herbarium specimens of Senecio nemorensis agg. indicated the occurrence of the following four species in Poland: S. germanicus Wallr., S. hercynicus Herborg, S. ovatus (G. Gaertn. et al. Willd. and S. ucranicus Hodálová. Principal component analysis (PCA, analysis of variance (ANOVA/Kruskal-Wallis test and canonical discriminant analysis (CDA were applied. Quantitative characters such as supplementary bract length, leaf base width, ligule length and the supplementary/involucral bract length ratio clearly discriminated taxa within S. nemorensis agg. Included is a distribution map of the investigated species based on the examined material, with particular emphasis on the course of the northeastern boundary of S. hercynicus and the northwestern boundary of S. ucranicus. Also given is a determination key for species within S. nemorensis agg. in Poland, together with morphological descriptions of particular species

  6. Chemical Diversity Investigation of Hepatotoxic Pyrrolizidine Alkaloids in Qianliguang (Senecio scandens and Related Species by UHPLC-QTOF-MS

    Directory of Open Access Journals (Sweden)

    Lin Zhu

    2015-04-01

    Full Text Available Objective: Qianliguang (Senecio scandens is a common Chinese medicinal herb. Qianliguang-containing herbal proprietary products are registered as over-the-counter remedies in China and exported to Western countries. The presence of hepatotoxic pyrrolizidine alkaloids (PAs has raised concerns about the safety of using Qianliguang and its products. The present study aims at investigation of different types of PAs present in Qianliguang collected from representative locations in China.

  7. Contribution to the Knowledge of the Alien Flora from Romania: Rudbeckia triloba L. and Senecio inaequidens DC.

    Directory of Open Access Journals (Sweden)

    Culita SIRBU

    2010-06-01

    Full Text Available Two alien species new to the Romanian flora, namely Rudbeckia triloba L. and Senecio inaequidens DC (Asteraceae family have been recorded during 2004-2009 period in different places of Moldavia and Maramures provinces (Romania. Rudbeckia triloba, native of North America, is a perennial species, 150 cm of length, with glabrate stems, blades ovate to subcordate or elliptic leaves, which present serrate margins. The capitula (10-30 is long pedunculate, with conic to subhemispheric receptacles (8-150-15 mm; with the paleae dark purple (5-6.5 mm. The ray florets are 7-15; the laminae is yellow-orange, linear to oblanceolate, 8-17 3-8 mm. The other species, Senecio inaequidens, is native of South Africa and it is a perennial herb. The cross pollination with non-specialized insects ensures the production of a large number of seeds, but self-pollination populations have also been reported, increasing the chances of the species to colonize new territory, where other sources of pollen were missing. Though for the moment these two species are sporadically met among the Romanian flora, at least one of them (Senecio inaequidens might become in the near future a threat to the natural or agricultural national ecosystems.

  8. Essential oil composition of Senecio graciliflorus DC: comparative analysis of different parts and evaluation of antioxidant and cytotoxic activities.

    Science.gov (United States)

    Lone, Shabir H; Bhat, Khursheed A; Bhat, Haroon M; Majeed, Rabiya; Anand, Rajneesh; Hamid, Abid; Khuroo, Mohd A

    2014-05-15

    The essential oil of different parts of Senecio graciliflorus DC was obtained by hydrodistillation and analysed by GC-FID and GC-MS for the first time. A total of 17, 20, 19 and 17 constituents were identified comprising 99.90, 95.50, 98.93 and 95.96% of the essential oil of flower, leaf, stem and root parts of Senecio graciliflorus respectively. Monoterpene hydrocarbons predominated in the essential oil with 85.28% in flower, 57.53% in leaf, 67.74% in stem and 64.98% in root oil. α-pinene, cis-ocimene, 1,2,3-trimethylcyclohexane and β-pinene were the major constituents of the essential oil. The flower essential oil exhibited a strong antioxidant potential displaying IC50 values of 21.6±0.6 and 26.0±1.0μg/ml in DPPH and hydroxyl radical assays respectively. On the other hand the essential oil of flower and root displayed highest cytotoxicity against lung (A-549) cancer cell lines (IC50=19.1±0.9 and 21.3±1.1μg/ml respectively. This study which represents the first report of the essential oil composition and bioevaluation of Senecio graciliflorus, can serve as a new source of cytotoxic and antioxidant activity.

  9. Lupus vulgaris of external nose.

    Science.gov (United States)

    Bhandary, Satheesh Kumar; Ranganna, B Usha

    2008-12-01

    Lupus vulgaris is the commonest form of cutaneous tuberculosis which commonly involve trunk and buttocks. Lupus vulgaris affecting nose and face, are rarely reported in India. This study reports an unusual case of lupus vulgaris involving the external nose that showed dramatic outcome after six months of anti- tubercular treatment.

  10. Lupus vulgaris: difficulties in diagnosis.

    Science.gov (United States)

    Rhodes, Julia; Caccetta, Tony Philip; Tait, Clare

    2013-05-01

    Lupus vulgaris is one of the most common forms of cutaneous tuberculosis. It presents a diagnostic challenge due to its paucibacillary nature. This is a report of a case of a delayed diagnosis of lupus vulgaris, presenting as perianal and peristomal plaques, followed by a review of the diagnostic tools for lupus vulgaris and their limitations.

  11. 千里光属(Senecio)13种植物的形态和组织学特征%Macroscopic and microscopic identification of Chinese herb belonging to genus Senecio

    Institute of Scientific and Technical Information of China (English)

    杨雪晶; 杨莉; 徐红; 张勉; 王峥涛

    2011-01-01

    The medicinal herbs derived from genus Senecio have been commonly used in Chinese medicine and triggered attention in recent decades for that they contain the hepatotoxic pyrrolizidine alkaloids. Therefore the botanical pharmacognostic study to authenticate those herbs based on their macroscopic and microscopic characteristics is important for the assurance of safety when they are applied as raw material for extracts or for finished products. In this paper, 13 taxa (11 species and 2 varieties) of Senecio plants were collected and their macroscopic and microscopic characteristics were observed and described by digital microscopic illustration. The results showed that the distribution of collenchyma in the cortex, the level of development for pericycle, the location of the phloem, and the ratio of pith in transverse sections of the stems, and the morphology of the leaf epidermal cells, the stomatal types and the non-glandular hairs in leaf surface view were found to be the main microscopic characteristics for authentication of different Senecio species. The herbs derived from genus Senecio can be distinguished from each other on the basis of their macroscopic and microscopic characteristics, and those observation can be used for the identification of commercial crude drugs from Senecio plants.%千里光在很多中成药中应用,但因其含有肝毒性吡咯里西啶生物碱而引起国际上的关注.为保证临床用药的安全,本文对千里光属11种、2变种药用植物的形态组织学特征进行了鉴定研究,了解千里光属植物的显微鉴定特征,为该类生药的商品鉴定提供依据.在性状鉴定的基础上,对千里光属生药茎横切面、叶表皮形态进行了观察比较.千里光属药材在生药性状、茎横切面、叶表面垂周壁观形态特征具有不同程度的差异,可以明显鉴别13种生药,为"千里光类"生药的鉴定提供依据.

  12. Psoriasiform lupus vulgaris.

    Science.gov (United States)

    Padmavathy, L; Rao, L Lakshmana; Ethirajan, N; Dhanlaklshmi, M

    2008-04-01

    Tuberculosis is a major public health problem in both developing and developed countries. Cutaneous Tuberculosis constitutes a minor proportion of extra-pulmonary manifestations of Tuberculosis. Lupus Vulgaris (LV) is one of the clinical variants of Cutaneous Tuberculosis. A case of a large plaque type psoriasiform lesion of lupus vulgaris on the thigh, of 15 years' duration, in an 18-year-old girl is reported. This case highlights the ignorance level among the patients and consequent failure to avail proper anti-tuberculous treatment despite campaign in print and audio visual media.

  13. Strongylus vulgaris and colic

    DEFF Research Database (Denmark)

    Nielsen, Martin Krarup; Jacobsen, Stine; Olsen, Susanne Nautrup

    Strongylus vulgaris is regarded the most pathogenic helminth parasite infecting horses. It was once estimated to be the primary cause of colic in horses and has been termed the horse killer. Disease is ascribed to thromboembolism caused by larvae migrating in the mesenteric arteries eventually le...

  14. Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya

    Directory of Open Access Journals (Sweden)

    Kiev Ochoa Pumaylle

    2012-12-01

    Full Text Available The aim of this work was extract, characterize and evaluate the antibacterial activity of the essential oil of Senecio graveolens Wedd (Wiskataya against Escherichia coli and Staphylococcus aureus ATCC 29923. The leaves and stems of S. graveolens were collected in the district of Puquio 3800 m.s.n.m., Lucanas province, and department of Ayacucho. The essential oil was obtained by steam water destilation dried leaves and stems with yield 1.26% (w/w to which physical testing were performed. The chemical composition was evaluated by gas chromatography with mass detector (GC-MS. Antibacterial activity of S. graveolens oil was tested by agar diffusion method in wells against Gram positive strains such as S. aureus ATCC 29923 and Gram negative as E. coli. The density a 20 ºC was 0.8755 g/ml; the index refraction was 1.4726 and the rate rotation was 102° 85' and the solubility miscible in ethanol. The GC-MS showed the main components sabinene (52.39 %, (+-4-carene (8.20 %, τ-terpinen (7.11 %, β-myrcene (6.74 %, 4-terpinenol (3.78 % and pulegone (3.67 %. The results showing activity strong antibacterial activity and moderate, respectively, for the strains tested, observing formation of inhibition halos for essential oil concentrations at 80, 90 and 100 % in both strains. The essential oil of S. graveolens presented with promising antibacterial activity.

  15. Demographic history of speciation in a Senecio altitudinal hybrid zone on Mt. Etna.

    Science.gov (United States)

    Filatov, Dmitry A; Osborne, Owen G; Papadopulos, Alexander S T

    2016-06-01

    Hybrid zones typically form as a result of species coming into secondary contact, but can also be established in situ as an ecotonal hybrid zone, a situation which has been reported far less frequently. An altitudinal hybrid zone on Mount Etna between two ragwort species (the low elevation Senecio chrysanthemifolius and high elevation S. aethnensis) could potentially represent either of these possibilities. However, a scenario of secondary contact vs. speciation with gene flow has not been explicitly tested. Here, we test these alternatives and demonstrate that the data do not support secondary contact. Furthermore, we report that the previous analyses of speciation history of these species were based on admixed populations, which has led to inflated estimates of ongoing, interspecific gene flow. Our new analyses, based on 'pure' S. aethnensis and S. chrysanthemifolius populations, reveal gene exchange of less than one effective migrant per generation, a level low enough to allow the species to accumulate neutral, genomewide differences. Overall, our results are consistent with a scenario of speciation with gene flow and a divergence time which coincides with the rise of Mt. Etna to altitudes above 2000 m (~150 KY). Further work to quantify the role of adaptation to contrasting environments of high and low altitudes will be needed to support the scenario of recent ecological speciation in this system.

  16. Diversity of pyrrolizidine alkaloids in native and invasive Senecio pterophorus (Asteraceae): implications for toxicity.

    Science.gov (United States)

    Castells, Eva; Mulder, Patrick P J; Pérez-Trujillo, Míriam

    2014-12-01

    Changes in plant chemical defenses after invasion could have consequences on the invaded ecosystems by modifying the interactions between plants and herbivores and facilitating invasion success. However, no comprehensive biogeographical studies have yet determined the phenotypic levels of plant chemical defenses, as consumed by local herbivores, covering large distributional areas of a species. Senecio pterophorus is a perennial shrub native to Eastern South Africa, expanded into Western South Africa and introduced into Australia and Europe. As other Asteraceae, S. pterophorus contains pyrrolizidine alkaloids (PAs) toxic to vertebrate and invertebrate herbivores. Here we analyzed S. pterophorus PAs by LC-MS/MS on foliage sampled across its entire distributional range, including the native and all non-native areas. PA concentrations and diversity was very high: we found 57 compounds belonging to 6 distinct necine base-types, including the highly toxic 1,2-unsaturated PAs (retronecine and otonecines) and the less toxic 1,2-saturated PAs (platynecine and rosmarinecines). Plants from different origins diverged in their PA absolute and relative concentrations. Rosmarinine was the most abundant compound in Australia and South Africa, but it was nearly absent in Europe. We characterized three plant chemotypes: retrorsine-senkirkine chemotype in Eastern South Africa, rosmarinine chemotype in Australia and Western South Africa, and acetylseneciphylline chemotype in Europe. PA absolute concentrations were highest in Australia. The increased absolute and relative concentrations of retronecine PAs from Australia and Europe, respectively, indicate that S. pterophorus is potentially more toxic in the invasive range than in the native range.

  17. Senecio brasiliensis (Asteraceae poisoning in Murrah buffaloes in Rio Grande do Sul Intoxicação natural por Senecio brasiliensis (Asteraceae em bubalinos no Rio Grande do Sul

    Directory of Open Access Journals (Sweden)

    André M.R. Corrêa

    2008-03-01

    Full Text Available Thirteen (14.4% out of 90 Murrah buffaloes (Bubalus bubalis became ill after con-sumption of Senecio brasiliensis, and 11 (12.2% of them died. The buffaloes were kept in a highly Senecio brasiliensis infested area. The poisoning occurred in June-August 2006 on a farm in the county of Nova Prata, Rio Grande do Sul, southern Brazil. Clinical signs included weakness, apathy, progressive weight loss, permanent decubitus, and diarrhea. Necropsy was performed in 2 of the 11 buffaloes that died. The pathological findings were typical of poisoning by pyrrolizidine alkaloids. High infestation of pastures with S. brasiliensis, severe drought, and consequent starvation were the main epidemiological factors associated with the poisoning here described.Descreve-se a ocorrência de um surto de intoxicação espontânea por Senecio brasiliensis em búfalos Murrah (Bubalus bubalis em uma propriedade localizada no município de Nova Prata, Estado do Rio Grande do Sul, no período de junho a agosto de 2006. De um total de 90 búfalos, 13 adoeceram e 11 morreram. Os animais eram mantidos em áreas de pastoreio altamente infestadas por S. brasiliensis. Os principais sinais clínicos relatados foram letargia, apatia, emagrecimento progressivo, diarréia e decúbito permanente. Necropsia foi feita em dois dos 11 animais mortos. As lesões foram características de intoxicações por alcalóides pirrolizidínicos. A grande quantidade da planta, forte estiagem e desnutrição conseqüente foram os principais achados epidemiológicos associados com a mortalidade.

  18. Composição e atividade antibacteriana dos óleos essenciais de Senecio crassiflorus var. crassiflorus Composition and antibacterial activity of the essential oils of Senecio crassiflorus var. crassiflorus

    Directory of Open Access Journals (Sweden)

    Anelise Levay Murari

    2008-01-01

    Full Text Available The essential oils of leaves, aerial and underground stems of Senecio crassiflorus var. crassiflorus were extracted by hydrodistillation and their analyses were performed by GC/MS. Qualitative and quantitative differences have been found between the analyzed parts. The main compounds of the essentials oils of the leaves were found to be oxygenated sesquiterpenes, while the oils from the aerial and underground stems showed sesquiterpene hydrocarbons as major constituents. Furthermore, the essential oils were tested against Staphylocccus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. The essential oil of the leaves was shown to be the most active.

  19. Lung and lupus vulgaris.

    Science.gov (United States)

    Mukta, V; Jayachandran, K

    2011-04-01

    Lupus vulgaris is chronic, postprimary, paucibacillary cutaneous tuberculosis found in individuals with moderate immunity and high degree of tuberculin sensitivity. Eighty percent of the lesions are on the head and neck. We present the case of a 38 year old lady who was admitted with complaints of worsening breathlessness and low grade fever of one month duration. Examination showed multiple, nontender skin ulcers on bilateral lumbar areas, two oozing serosanguinous discharge and others scarred in the centre. Respiratory system examination and chest X-ray revealed right sided pleural effusion. On investigation, pleural fluid was tuberculous in nature. Skin biopsy from the edge of ulcer was also suggestive of tuberculosis. Patient is doing well on antituberculous drugs. This case highlights the importance of cutaneous manifestations of systemic disease and is an example of the unusual presentation of lupus vulgaris in a case of pleural effusion.

  20. Pemphigus vulgaris in pregnancy.

    Science.gov (United States)

    Salzberg, Kelsey W; Gero, Melanie J; Ragsdale, Bruce D

    2014-10-01

    We report the case of a 34-year-old woman who was diagnosed with pemphigus vulgaris (PV) during pregnancy. The patient presented with widespread blistering dermatitis and associated burning and pruritus. At 6 weeks' gestation the patient was admitted to the hospital to expedite her diagnosis and initiate treatment. A skin biopsy revealed suprabasal acantholysis, and direct immunofluorescence demonstrated diffuse intercellular IgG in the epidermis and basal intercellular C3, which confirmed the diagnosis of PV. Treatment with corticosteroids was instituted after discussions with the patient about possible adverse effects to the fetus. Pemphigus vulgaris is rare in pregnancy and active PV presents potential threats of fetal spread and transient lesion production, which is associated with increased mortality and morbidity in the fetus. Our patient had active PV and required treatment throughout her pregnancy. The pregnancy progressed to premature delivery of the neonate without skin lesions or apparent complications.

  1. Depsides from Prunella vulgaris

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A novel depsides 1, together with three known ones 2~4 and two phenylpropanoids 5~6 were isolated from the ethanol extract of the spikes of Prunella vulgaris. On the basis of spectral and chemical evidence, their structures were deternined as butyl rosmarinate 1, ethyl rosmarinate 2, methyl rosmarinate 3, rosmarinic acid 4, 3,4,a -trihydroxy-methyl phenylpropionate 5 and p-coumaric acid 6, respectively.

  2. Intoxicação experimental por Senecio brasiliensis (Asteraceae em eqüinos Experimental poisoning by Senecio brasiliensis (Asteraceae in horses

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    Célso Pilati

    2007-07-01

    Full Text Available As partes aéreas dessecadas de Senecio brasiliensis (Spreng. colhidas em estágio de brotação foram administradas a 14 eqüinos de raça mista, com idades de 4-22 anos e pesos de 230-475 kg. Um eqüino de 15 anos, que não recebeu a planta, serviu de controle. Pequenas quantidades da planta eram misturadas à ração oferecida aos eqüinos; quantidades maiores eram moídas, misturadas em água e administradas por sonda nasogástrica. Biópsias hepáticas foram periodicamente realizadas em 11 eqüinos. Nove eqüinos morreram com sinais ou lesões da intoxicação após receberem quantidades da planta correspondente a 0,87%, 1,5% (administrações únicas, 1,74% (duas administrações semanais, 3.0% (três administrações diárias, 7.42% (17 administrações semanais, 8.9% (284 administrações diárias, 9.66% (82 administrações diárias e 9,30% (43 administrações semanais de seus pesos corporais. Dois eqüinos que receberam quantidades da planta correspondentes a 15,0% (30 e 60 administrações diárias de seus pesos corporais morreram durante o experimento por causas não relacionadas à intoxicação. Três eqüinos que receberam quantidades da planta correspondentes a 0,5% e 1.0% (administrações únicas, 15.0% (240 administrações diárias de seus pesos corporais e o eqüino controle sobreviveram sem apresentar sinais clínicos. A doença clínica induzida pela planta teve uma evolução de 1-30 dias e foi caracterizada por anorexia, icterícia, e sinais neurológicos de encefalopatia hepática. Perda de peso foi observada nos casos de evolução clínica mais longa. Os achados de necropsia incluíam acentuação do padrão lobular na superfície natural e de corte dos fígados ou eram firmes e vermelho-escuros. Hemorragias disseminadas eram freqüentes, mas mais conspícuas no tecido subcutâneo, superfícies serosa e mucosa do trato gastrintestinal. Edema era observado na submucosa do trato gastrintestinal e nas cavidades org

  3. Rapid and High Seed Germination and Large Soil Seed Bank of Senecio aquaticus in Managed Grassland

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    Matthias Suter

    2012-01-01

    Full Text Available Senecio aquaticus, regionally a Red List species in Europe, has become increasingly abundant in agricultural grassland of medium to high management intensity in Switzerland, Southern Germany, and Austria in recent years, where it is a threat for animal and human health due to its toxicity. In this study, we investigated the seed ecology of S. aquaticus to help protection of the species in relic populations while improving its control when abundant in managed grassland. Germination percentages of fresh ripe seeds of S. aquaticus were on average 68% in 2008, but only 45% in 2010, indicating yearly variation. Germination was generally fast: ten days after the onset of the tests, often more than 45% of all seeds had germinated. When covered with a soil layer of 5 mm, germination was only 16% compared to 63% in full light. Seeds buried in the soil for one and two years showed a germination of 78%, significantly higher than that of fresh ripe seeds, thus suggesting a stimulating effect of cold-wet stratification on germination and long seed survival in the soil. In grasslands with established populations of S. aquaticus, the number of germinable seeds of the species ranged from 361 to 1875 m-2 in topsoil (0–10 cm with an average of 1139 m-2. The large seed bank and the rapid and high germination of S. aquaticus suggest that allowing seed formation is important for its preservation in relic populations. With respect to agricultural grassland, strategies to control the species should initially target hindering seed production and dispersal.

  4. Arbuscular mycorrhizal colonization, plant chemistry, and aboveground herbivory on Senecio jacobaea

    Science.gov (United States)

    Reidinger, Stefan; Eschen, René; Gange, Alan C.; Finch, Paul; Bezemer, T. Martijn

    2012-01-01

    Arbuscular mycorrhizal fungi (AMF) can affect insect herbivores by changing plant growth and chemistry. However, many factors can influence the symbiotic relationship between plant and fungus, potentially obscuring experimental treatments and ecosystem impacts. In a field experiment, we assessed AMF colonization levels of individual ragwort ( Senecio jacobaea) plants growing in grassland plots that were originally sown with 15 or 4 plant species, or were unsown. We measured the concentrations of carbon, nitrogen and pyrrolizidine alkaloids (PAs), and assessed the presence of aboveground insect herbivores on the sampled plants. Total AMF colonization and colonization by arbuscules was lower in plots sown with 15 species than in plots sown with 4 species and unsown plots. AMF colonization was positively related to the cover of oxeye daisy ( Leucanthemum vulgare) and a positive relationship between colonization by arbuscules and the occurrence of a specialist seed-feeding fly ( Pegohylemyia seneciella) was found. The occurrence of stem-boring, leaf-mining and sap-sucking insects was not affected by AMF colonization. Total PA concentrations were negatively related to colonization levels by vesicles, but did not differ among the sowing treatments. No single factor explained the observed differences in AMF colonization among the sowing treatments or insect herbivore occurrence on S. jacobaea. However, correlations across the treatments suggest that some of the variation was due to the abundance of one plant species, which is known to stimulate AMF colonization of neighbouring plants, while AMF colonization was related to the occurrence of a specialist insect herbivore. Our results thus illustrate that in natural systems, the ecosystem impact of AMF through their influence on the occurrence of specialist insects can be recognised, but they also highlight the confounding effect of neighbouring plant species identity. Hence, our results emphasise the importance of field

  5. Floral biology of Senecio macrophyllus M. BIEB. (Asteraceae, a rare Central European steppe plant

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    Bożenna Czarnecka

    2014-03-01

    Full Text Available Knowledge of the flowering phase and plant pollination ecology is very important for understanding the life history of long-lived perennials. In the case of rare species, the information may have implications for conservation practices. Our studies on flower morphology and blooming biology of the vulnerable plant Senecio macrophyllus M. BIEB. were conducted in situ (flowering, activity of insect visitors in the largest population in SE Poland and in laboratory (light and scanning electron microscopy. The disc florets open diurnally with most intensive anthesis in the early afternoon and attract insect visitors with nectar and pollen. In highly protandrous disc florets, pollen viability decreases in time, whereas stigma receptivity increases. The upper part of the pistil forms a brush-like pollen presenter, covered with unicellular trichomes with chromoplasts. Pollen presentation lasts 4–11 hours per floret, and 8 days in a single head, the main function of which is to extend the availability of male gamets for reproduction. The number of florets per head, the head size, and the number of pollen grains produced per anther, and the pollen grain viability differed significantly between microsites. The finding indicates that, apart from the biotic factors, abiotic conditions may considerably influence generative reproduction of the species. In the study area, the principal visitors of S. macrophyllus flowers were Hymenoptera, with predominance of Apis mellifera (53.4% of visits. The remarkable share of butterflies (13.9% recognized as the long-distance moving insects may improve the genetic variability of individuals within a fragmented population. A more detailed study is necessary to explain the role of insect visitors in effective pollination and in gene transfer between patches. The assessment of optimal conditions for the generative reproduction is fundamental for the in situ conservation of this rare species.

  6. The role of propagule pressure, genetic diversity and microsite availability for Senecio vernalis invasion.

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    Alexandra Erfmeier

    Full Text Available Genetic diversity is supposed to support the colonization success of expanding species, in particular in situations where microsite availability is constrained. Addressing the role of genetic diversity in plant invasion experimentally requires its manipulation independent of propagule pressure. To assess the relative importance of these components for the invasion of Senecio vernalis, we created propagule mixtures of four levels of genotype diversity by combining seeds across remote populations, across proximate populations, within single populations and within seed families. In a first container experiment with constant Festuca rupicola density as matrix, genotype diversity was crossed with three levels of seed density. In a second experiment, we tested for effects of establishment limitation and genotype diversity by manipulating Festuca densities. Increasing genetic diversity had no effects on abundance and biomass of S. vernalis but positively affected the proportion of large individuals to small individuals. Mixtures composed from proximate populations had a significantly higher proportion of large individuals than mixtures composed from within seed families only. High propagule pressure increased emergence and establishment of S. vernalis but had no effect on individual growth performance. Establishment was favoured in containers with Festuca, but performance of surviving seedlings was higher in open soil treatments. For S. vernalis invasion, we found a shift in driving factors from density dependence to effects of genetic diversity across life stages. While initial abundance was mostly linked to the amount of seed input, genetic diversity, in contrast, affected later stages of colonization probably via sampling effects and seemed to contribute to filtering the genotypes that finally grew up. In consequence, when disentangling the mechanistic relationships of genetic diversity, seed density and microsite limitation in colonization of

  7. Chemical Diversity Investigation of Hepatotoxic Pyrrolizidine Alkaloids in Qianliguang (Senecio scandens) and Related Species by UHPLC-QTOF-MS1

    Institute of Scientific and Technical Information of China (English)

    Lin Zhua; Na Li; Jian-Qing Ruan; Peter P. Fu; Zhong-Zhen Zhao; Ge Lina

    2015-01-01

    Objective: Qianliguang (Senecio scandens) is a common Chinese medicinal herb. Qianliguang-containing herbal proprietary products are registered as over-the-counter remedies in China and exported to Western countries. The presence of hepatotoxic pyrrolizidine alkaloids (PAs) has raised concerns about the safety of using Qianliguang and its products. The present study aims at investigation of different types of PAs present in Qianliguang collected from representative locations in China. Methods: In this study, a simple but specific UHPLC-QTOF-MS method for the determination of toxic PAs was developed, based on the characteristic fragment ions specific to different types of PAs. It was successfully applied for the identification and distinguishing of PAs present in Qianliguang and related Senecio species growing in different locations of China. Results: Significant diversity of the PA types and quantities were revealed among the samples tested. The estimated total amounts of toxic PAs in three of the samples exceed the toxic limits of PA intake restricted by WHO, demonstrating the timely and highly demand for regulating both types and quantities of PAs present in Qianliguang. Conclusions: This study provides the methodology for simultaneous identification and quantification of PAs present in herbs without requiring corresponding standards, which could be further used for more systematic investigations of the PA distribution in Qianliguang and other PA-containing herbs.

  8. Thymus vulgaris subsp. mansanetianus subsp. nov. (Lamiaceae)

    OpenAIRE

    Pedro Pablo Ferrer-Gallego; Albert J. Navarro Peris; Emilio Laguna Lumbreras; Gonzalo Mateo Sanz

    2013-01-01

    RESUMEN: Se describe una nueva subespecie de Thymus vulgaris L. (Lamiaceae); Th. vulgaris subsp. mansanetianus subsp. nov., caracterizada por presentar un hábito postrado, tallos estoloníferos, decumbentes y radicantes, hojas muy estrechas y una floración otoñal. ABSTRACT: Thymus vulgaris subsp. mansanetianus subsp. nov. (Lamiaceae). A new subspecies of Thymus vulgaris L. (Lamiaceae); Th. vulgaris subsp. mansanetianus subsp. nov. is described. This new subspecies is characterized by its prost...

  9. Anti-diabetic activity of the chloroform extract of Senecio mikanioides Otto in streptozotocin induced diabetic rats

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    Hanan S. M. Marzouk

    2011-08-01

    Full Text Available Objective: In this study, we examined the effect of oral administration of the chloroform extract of Senecio mikanioides Otto for a period of 30 days at doses of 100, 300 or 500 mg/kg body weight/day on streptozotocin induced diabetic rats. Methods: Male Sprague-Dawley rats were divided into five groups and classified into; extract treatment group received 100, 300 or 500 mg/kg body weight per day by oral gavage for 30 days; diabetic group administered intraperitoneally a single dose (75 mg/kg body weight of streptozotocin (STZ; diabetic rats plus tolbutamide group where diabetic rats received the standard hypoglycemic drug tolbutamide (100 mg/kg body weight per day by oral gavage for 30 days; diabetic rats plus extract group where diabetic rats received the extract (same doses by gavage for 30 days; as well as a normal group for comparison. In all of these groups, the levels of glucose and insulin were checked in blood, while the levels of reduced glutathione (GSH, nitric oxide (NO, lipid peroxidation (TBARS and thioredoxin reductase (TrxR activity were measured in pancreatic tissues. Results: The results revealed that STZ administration resulted in significant elevation in the level of both TBARS and NO with depletion in the level of GSH as compared with control accompanied with hyperglycemia, hypo-insulinemia and low insulin sensitivity. Moreover, the activity of pancreatic TrxR was lower than the control group. Feeding the diabetic rats for 30 days with the extract normalizes the previous biochemical parameters in dose dependent manner reaching near the tolbutamide treated group at the highest dose. Conclusion: The chloroform extract of Senecio mikanioides Otto exhibited antidiabetic activity and it corrected the insulin level and its sensitivity in experimentally induced diabetic rats in dose dependent manner. The current results clearly indicated the beneficial effects of the chloroform extract of Senecio mikanioides Otto in both

  10. The immunogenetics of pemphigus vulgaris.

    Science.gov (United States)

    Gazit, Ephraim; Loewenthal, Ron

    2005-01-01

    Pemphigus vulgaris (PV) is an autoimmune blistering disease of the skin of unknown etiology. While various environmental factors have been implicated as triggering agents, HLA association is probably the most important predisposing factor. The aim of this review is to highlight the association of HLA with pemphigus vulgaris. In addition, we present recent results showing a possible association with the nonclassical HLA-G antigen.

  11. Nail manifestations in pemphigus vulgaris

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    Cahali Juliana Burihan

    2002-01-01

    Full Text Available Nail involvement in pemphigus vulgaris is rare. We describe 5 patients with pemphigus vulgaris presenting nail involvement. In this disease, nail manifestations present, by order of frequency, as chronic paronychia, onychomadesis, onycholysis, Beau's lines and trachyonychia. All our 5 cases presented with paronychia, and 1 of them also had Beau's lines. Treatment with prednisone and/or cyclophosphamide controlled mucocutaneous and nail manifestations in all cases.

  12. Loss of pyrrolizidine alkaloids on decomposition of ragwort (Senecio jacobaea) as measured by LC-TOF-MS.

    Science.gov (United States)

    Crews, Colin; Driffield, Malcolm; Berthiller, Franz; Krska, Rudolf

    2009-05-13

    The decomposition of toxic pyrrolizidine alkaloids in ragwort (Senecio jacobaea) on storage in waste bags has been evaluated by a new time-of-flight mass spectrometric detection method. The method makes progress in meeting the clear need for modern analytical methods for pyrrolizidine alkaloids and for studies into factors affecting the stability of the toxins in the uprooted plant, which might still be accessible to animals. The experiments demonstrated a rapid decomposition of the toxins in ragwort stored in bags, from 340 mg/kg to less than 40 mg/kg in four weeks and virtually complete loss after 10 weeks. The information obtained can guide effective ragwort removal procedures to safeguard grazing animals.

  13. Actividad biológica de la raíz de senecio salignus contra zabrotes subfasciatus en frijol almacenado

    OpenAIRE

    Ernesto López Pérez; Cesáreo Rodríguez Hernández; Laura D. Ortega Arenas; Ramón Garza García

    2007-01-01

    El brúquido Zabrotes subfasciatus es la principal plaga del frijol almacenado en zonas cálidas, y puede ocasionar daño total si no se controla. Se realizó una serie de experimentos en confinamiento y libre elección en condiciones controladas para conocer la actividad biológica del polvo de la raíz de chilca Senecio salignus en Z. subfasciatus, utilizando un diseño completamente al azar. El polvo al 0.01, 0.05, 0.07, 0.1, 0.25, 0.5 y 1% o con tamaños 0.25, 0.25-0.99, 1-2, y 4 mm se mezcló con ...

  14. PERFIL SOROLÓGICO EM EQUINOS INTOXICADOS EXPERIMENTALMENTE POR SENECIO BRASILIENSIS COMPOSITAE: PARTE II BILIRRUBINAS, ALBUMINAS E PROTEÍNAS

    OpenAIRE

    Sonia Terezinha dos Anjos Lopes; Luis Carlos Ribeiro Fan; Celso Pilati; Maria Luchese

    1994-01-01

    RESUMO As parte aéreas dessecadas de senecio brasiliensis, colhidas no período de brotação, foram administradas a 14 equinos de raça mista, com idade de 4 a 22 anos e pesos de 230 a 475kg. A planta era misturada à ração dada aos animais ou moída, misturada em água e administrada por sonda nasoesofágica. Foram realizadas colheitas de sangue periodicamente nos 14 equinos para avaliação dos níveis séricos de bilirrubina, albumina e proteína. Oito cavalos desenvolveram a intoxicação, três morrera...

  15. [Acne vulgaris: endocrine aspects].

    Science.gov (United States)

    Dekkers, O M; Thio, B H; Romijn, J A; Smit, J W A

    2006-06-10

    Androgens play an important part in the development of acne vulgaris. Androgen levels in patients with acne are higher than those in controls and people with the androgen insensitivity syndrome do not develop acne. Local factors other than androgen plasma levels, also play a part in the development of acne. The skin contains enzymes that convert precursor hormones to the more potent androgens such as testosterone and dihydrotestosterone. Androgen synthesis can therefore be regulated locally. The effects of androgens on the skin are the result of circulating androgens and enzyme activity in local tissues and androgen receptors. Acne is a clinical manifestation of some endocrine diseases. The polycystic ovary syndrome has the highest prevalence. In women with acne that persists after puberty, in 10-200% of cases polycystic ovary syndrome is later diagnosed. The mechanism of hormonal anti-acne therapy may work by blocking the androgen-production (oestrogens) or by blocking the androgen receptor (cyproterone, spironolactone).

  16. Occasional hybridization between a native and invasive Senecio species in Australia is unlikely to contribute to invasive success.

    Science.gov (United States)

    Dormontt, Eleanor E; Prentis, Peter J; Gardner, Michael G; Lowe, Andrew J

    2017-01-01

    Hybridization between native and invasive species can facilitate introgression of native genes that increase invasive potential by providing exotic species with pre-adapted genes suitable for new environments. In this study we assessed the outcome of hybridization between native Senecio pinnatifolius var. pinnatifolius A.Rich. (dune ecotype) and invasive Senecio madagascariensis Poir. to investigate the potential for introgression of adaptive genes to have facilitated S. madagascariensis spread in Australia. We used amplified fragment length polymorphisms (141 loci) and nuclear microsatellites (2 loci) to genotype a total of 118 adults and 223 seeds from S. pinnatifolius var.pinnatifolius and S. madagascariensis at one allopatric and two shared sites. We used model based clustering and assignment methods to establish whether hybrid seed set and mature hybrids occur in the field. We detected no adult hybrids in any population. Low incidence of hybrid seed set was found at Lennox Head where the contact zone overlapped for 20 m (6% and 22% of total seeds sampled for S. pinnatifolius var. pinnatifolius and S. madagascariensis respectively). One hybrid seed was detected at Ballina where a gap of approximately 150 m was present between species (2% of total seeds sampled for S. madagascariensis). We found no evidence of adult hybrid plants at two shared sites. Hybrid seed set from both species was identified at low levels. Based on these findings we conclude that introgression of adaptive genes from S. pinnatifolius var. pinnatifolius is unlikely to have facilitated S. madagascariensis invasions in Australia. Revisitation of one site after two years could find no remaining S. pinnatifolius var. pinnatifolius, suggesting that contact zones between these species are dynamic and that S. pinnatifolius var. pinnatifolius may be at risk of displacement by S. madagascariensis in coastal areas.

  17. Occasional hybridization between a native and invasive Senecio species in Australia is unlikely to contribute to invasive success

    Directory of Open Access Journals (Sweden)

    Eleanor E. Dormontt

    2017-08-01

    Full Text Available Background Hybridization between native and invasive species can facilitate introgression of native genes that increase invasive potential by providing exotic species with pre-adapted genes suitable for new environments. In this study we assessed the outcome of hybridization between native Senecio pinnatifolius var. pinnatifolius A.Rich. (dune ecotype and invasive Senecio madagascariensis Poir. to investigate the potential for introgression of adaptive genes to have facilitated S. madagascariensis spread in Australia. Methods We used amplified fragment length polymorphisms (141 loci and nuclear microsatellites (2 loci to genotype a total of 118 adults and 223 seeds from S. pinnatifolius var.pinnatifolius and S. madagascariensis at one allopatric and two shared sites. We used model based clustering and assignment methods to establish whether hybrid seed set and mature hybrids occur in the field. Results We detected no adult hybrids in any population. Low incidence of hybrid seed set was found at Lennox Head where the contact zone overlapped for 20 m (6% and 22% of total seeds sampled for S. pinnatifolius var. pinnatifolius and S. madagascariensis respectively. One hybrid seed was detected at Ballina where a gap of approximately 150 m was present between species (2% of total seeds sampled for S. madagascariensis. Conclusions We found no evidence of adult hybrid plants at two shared sites. Hybrid seed set from both species was identified at low levels. Based on these findings we conclude that introgression of adaptive genes from S. pinnatifolius var. pinnatifolius is unlikely to have facilitated S. madagascariensis invasions in Australia. Revisitation of one site after two years could find no remaining S. pinnatifolius var. pinnatifolius, suggesting that contact zones between these species are dynamic and that S. pinnatifolius var. pinnatifolius may be at risk of displacement by S. madagascariensis in coastal areas.

  18. PERFIL SOROLÓGICO EM EQUINOS INTOXICADOS EXPERIMENTALMENTE POR SENECIO BRASILIENSIS COMPOSITAE: PARTE II BILIRRUBINAS, ALBUMINAS E PROTEÍNAS

    Directory of Open Access Journals (Sweden)

    Sonia Terezinha dos Anjos Lopes

    1994-01-01

    Full Text Available RESUMO As parte aéreas dessecadas de senecio brasiliensis, colhidas no período de brotação, foram administradas a 14 equinos de raça mista, com idade de 4 a 22 anos e pesos de 230 a 475kg. A planta era misturada à ração dada aos animais ou moída, misturada em água e administrada por sonda nasoesofágica. Foram realizadas colheitas de sangue periodicamente nos 14 equinos para avaliação dos níveis séricos de bilirrubina, albumina e proteína. Oito cavalos desenvolveram a intoxicação, três morreram por causas outras e três sobreviveram sem apresentar quaisquer sinais clínicos durante o período em que foram observados. Nos animais que desenvolveram a doença os sinais clínicos tiveram evolução de um a seis dias e caracterizaram-se por anorexia, icterícia e distúrbios neurológicos. A bilirrubina total e indireta apresentam-se elevadas na fase terminal da intoxicação e a albumina e proteína mostram níveis abaixo dos valores de referência num equino que desenvolveu a forma crônica da enfermidade. Conclui-se que dentre as provas laboratoriais usadas, a bilirrubina indireta apresenta melhor indicação da alteração hepática causada por intoxicação pelo senecio brasiliensis.

  19. Effects of intercropping on growth and reproductive capacity of late-emerging Senecio vulgaris L., with special reference to competition for light

    NARCIS (Netherlands)

    Baumann, D.T.; Bastiaans, L.; Kropff, M.J.

    2001-01-01

    Due to increased emphasis on long-term management of weed populations in cropping systems with a reduced reliance on herbicides, the production of seeds by weeds that emerge after the critical period for weed control is increasingly important. It was hypothesized that increased soil cover and light

  20. Pemphigus Vulgaris Presented with Cheilitis

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    Zaheer Abbas

    2014-01-01

    Full Text Available Background. Pemphigus vulgaris is an autoimmune blistering disease affecting the mucous membrane and skin. In 50 to 70% of cases, the initial manifestations of pemphigus vulgaris are oral lesions which may be followed by skin lesions. But it is unusual for the disease to present with initial and solitary persistent lower lip lesions without progression to any other location. Main Observations. We report a 41-year-old woman with dry crusted lesions only on the lower lip, clinically resembling actinic cheilitis and erosive lichen planus, but histopathological evaluation showed unexpected results of suprabasal acantholysis and cleft compatible with pemphigus vulgaris. We treated her with intralesional triamcinolone 10 mg/mL for 2 sessions and 2 g cellcept daily. Patient showed excellent response and lesions resolved completely within 2 months. In one-year follow-up, there was no evidence of relapse or any additional lesion on the other sites. Conclusion. Cheilitis may be the initial and sole manifestation of pemphigus vulgaris. Localized and solitary lesions of pemphigus vulgaris can be treated and controlled without systemic corticosteroids.

  1. Variación en la producción de alcaloides en inflorescencias de Senecio Grisebachii por deficiencia de nutrientes Alkaloid production changes due to nutrient deficiencies in Senecio Grisebachii inflorescences

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    Margarita A Yaber Grass

    2009-06-01

    Full Text Available Senecio grisebachii Baker es una maleza que invade pasturas naturales y cultivos en el sur de Brasil, Uruguay, y provincias mesopotámicas y Buenos Aires en la Argentina y es considerada tóxica debido a la presencia de alcaloides pirrolizidínicos (APs en sus tejidos. Se evaluó el efecto de la deficiencia de nitrógeno y de fósforo sobre el perfil de APs en inflorescencias de esta maleza. Se realizó un ensayo utilizando un diseño completamente aleatorizado con 10 repeticiones, en el cual las plantas, que crecieron en hidroponia desde mayo hasta octubre, fueron regadas con solución de nutrientes en las que las concentraciones de N o de P reducidas en un 50% respecto del control. La cuantificación de APs en las inflorescencias de S. grisebachii por CG y CG-EM, demuestra un aumento significativo en el contenido total de APs, en los tratamientos con déficit en N o P (1,33 y 1,34 mg g-1 de materia seca, respectivamente, comparados con el control 0,35 mg g-1. Se identificaron siete APs y sus concentraciones variaron entre tratamientos. Senecionina resultó el alcaloide mayoritario en el tratamiento déficit de N, mientras senecifilina resultó más abundante en los tratamientos control y con déficit de P, seguidos en todos los casos por integerrimina y cantidades menores de espartiodina, jacobina, jacozina y retrorsina.Senecio grisebachii Baker is a weed that invades natural pastures and crops in southern Brazil, Uruguay, the mesopotamic provinces and Buenos Aires in Argentina, and is considered to be toxic because of the presence of pyrrolizidine alkaloids (PAs in its tissues. The effects of nitrogen and phosphorus deficiency was evaluated on the APs patterns of the weed inflorescences. A completely randomized design with 10 repetitions was used in an experiment where plants growing in hydroponics from May to October were irrigated with a nutrient solution containing 50% P or N concentrations with respect to the control treatment. PAs

  2. Signalling pathways in pemphigus vulgaris.

    Science.gov (United States)

    Li, Xiaoguang; Ishii, Norito; Ohata, Chika; Furumura, Minao; Hashimoto, Takashi

    2014-03-01

    Acantholysis in pemphigus vulgaris is induced by binding of autoantibodies to desmoglein 3 (Dsg3). The roles of signalling pathways on development of acantholysis have recently been extensively studied. In the study by Sayar et al., recently published in Exp Dermatol, epidermal growth factor receptor (EGFR) signalling was activated in both in vivo and in vitro pemphigus vulgaris experimental models. However, while EGFR inhibitors suppressed activity of p38 mitogen-activated protein kinase (p38MAPK) linearly, they suppressed activity of c-Myc and acantholysis in a non-linear, V-shaped relationship. These findings indicated complicated interactions among EGFR, p38MAPK and c-Myc in pemphigus vulgaris pathology.

  3. Atopic dermatitis and ichthyosis vulgaris.

    Science.gov (United States)

    Rabinowitz, L G; Esterly, N B

    1994-06-01

    Atopic dermatitis remains a common skin problem in the pediatric age group. General approaches to management focus on reducing inflammation and pruritus as well as preventing xerosis. Ichthyosis vulgaris is the most common form of the ichthyoses and often is associated with atopic dermatitis. Recognition of these conditions is necessary to institute therapy that will alleviate the discomfort experienced by affected individuals.

  4. Porokeratosis Masquerading As Lupus Vulgaris

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    Das Sudip

    2004-01-01

    Full Text Available Porokeratosis is a specific disorder of kerstinization characterized histologically by cornoid lamella. Lesions of porokeratosis present varied features, often mimicking other dermatological disorders. Herein, we report a case of porokeratosis of Mibelli presenting with verrucous lesions simulating lupus vulgaris.

  5. Disseminated lupus vulgaris.

    Science.gov (United States)

    Garg, Taru; Ramchander; Shrihar, Rashmi; Gupta, Tanvi Pal; Aggarwal, Shilpi

    2011-01-01

    follicular plugging and multiple epithelioid cell granulomas, rimmed by lymphocytes in the deeper portion of the dermis, mainly peri-appendageal. Stain for acid-fast bacteria was negative. Cultures from the skin lesions were negative. The patient was diagnosed as having lupus vulgaris with multiple lesions of varying morphology at different sites with pulmonary tuberculosis and healed lymph node involvement.

  6. Teste de especificidade hospedeira de Phaedon confinis (Coleoptera, Chrysomelidae, um potencial agente de biocontrole de Senecio brasiliensis (Asteraceae.

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    Julianne Milléo

    2011-07-01

    Abstract. Senecio brasiliensis (Spreng. Less when ingested by cattle and horses, the plant causes seneciosis, a serious poisoning. Due to the great financial losses to cattle ranchers, controlling the plant using insects has become attractive. Systematic survey efforts have revealed that Phaedon confinis Klug causes serious damage to the plant, and may be a great biocontrol agent. The object was to extend the tests of host specificity to 52 plants using 1st larval instar and adult chrysomelid bettles. The insects were submitted to “no-choice” and “multiple-choice” tests. The following results were obtained: “NO-CHOICE” L1 – 52 plants tested: null 90.39%; negligible damage 5.77%; light 1.92%; and normal in only S. brasiliensis 1.92%, where 31.67% of larvae obtained an adult phase. “NO-CHOICE” ADULTS – 46 plants. Null damage was recorded in 82.60%; 13.04% showed negligible damage; 2.17% light; 2.17% normal in S. brasiliensis. The chysomelids oviposited during observation days only on S. brasiliensis leaves. 615 eggs were oviposited with 73.01% viability. “MULTIPLE CHOICE” LARVAE – nine plants tested. 66.67% null; 11.11% weak; 11.11% negligible damage; and 11.11% normal in S. brasiliensis. The results indicate that the normal diet, oviposition, survival and development of P. confinis is restricted to S. brasiliensis and corroborates its potential as a biocontrol agent.

  7. Chemical Composition and Antimicrobial Activity of the Essential Oils from the Flower, Leaf, and Stem of Senecio pandurifolius

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    Gonca Tosun

    2011-01-01

    Full Text Available The essential oils from the fresh flower, leaf, and stem of Senecio pandurifolius (Asteraceae were isolated by hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of forty-five, sixty, and forty-two compounds were identified, constituting over 90.1 %, 88.0%, and 89.0% of oil composition of the flower, leaf, and stem of S. pandurifolius , respectively. The chemical profile reveals the dominance of sesquiterpene hydrocarbons (flower: 42.4%, leaf: 43.4%, stem: 52.3% . The main components of essential oils own to S. pandurifolius were α- cuprenene (30.7% in flower, α­- zingiberene (16.1% in leaf and γ -curcumene (14.9% in stem. Terpene related compounds were in minor amounts in all parts (flower: 1.4%, leaf: 1.5%, stem: 1.9% of the S. pandurifolius . Also there was no monoterpene hydrocarbons and oxygenated monoterpenes in the essential oil of the stem. In addition, antimicrobial activities of the essential oils of S. pandurifolius were investigated. The oils showed activity against Gram positive bacteria, mycobacterium and fungi, but not Gram negative bacteria. A high antimycobacterial activity was observed with leaf essential oil, which deserves further investigation to determine its active components.

  8. Multiple introductions and gene flow in subtropical South American populations of the fireweed, Senecio madagascariensis(Asteraceae

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    Geraldo Mäder

    2016-03-01

    Full Text Available Abstract Non-indigenous plants exhibit different attributes that make them aggressive competitors with indigenous plants and serious threats to biodiversity.Senecio madagascariensis (fireweed, Asteraceae, a native from southern Africa, is a strong competitor in agricultural activities and has toxic alkaloids that may result in high cattle mortality. In Brazil, this weed was collected for the first time in 1995 and has since spread quickly throughout the Pampas region. To better understand the invasion of the fireweed in South America, we used a genetic characterization with internal transcribed spacer (ITS and microsatellite markers. Based on the ITS data, the southern Brazil populations of S. madagascariensis shared genetic homology with samples taken from the Hawaiian Islands and South Africa. Microsatellite analysis showed the genetic diversity split in two clusters, perhaps intimating the independent introduction of each species into South America. Although fireweed was introduced recently in southern Brazil, the considerable levels of genetic diversity, gene flow, and inbreeding may indicate success in the species establishment in this environment.

  9. Analysis of the Causes of Senecio Scandens Clinical Adverse Reactions%千里光临床不良反应成因分析

    Institute of Scientific and Technical Information of China (English)

    尹利顺; 李晓宇; 孙蓉

    2015-01-01

    目的:分析千里光发生临床不良反应的原因,为进一步安全性评价提供文献依据和研究思路。方法通过近十多年来国内外相关文献对千里光临床不良反应的报道,进行整理、分析与归纳。结果千里光主要临床不良反应为肝脏损伤,其发生与长期使用、使用剂量、品种混用、配伍使用、制备工艺和个体差异等原因有关,尤其是长期服用是千里光致肝损伤、甚至产生死亡的主要原因;千里光所含的吡咯里西啶类生物碱(pyrrolizidine alkaloids, PAs)是导致肝毒性的主要物质基础。结论针对千里光临床不良反应,应亟待加强千里光药材及其中成药中肝损伤毒性规律和物质基础研究,进而通过明晰肝毒物质基础的体内过程和“TD-TK”关联评价,制订体内安全限度,完善并提高质量标准,保证临床用药安全。%Objective To review the research status of Senecio scandens clinical adverse reactions, in order to offer accordance and study ideas for further safety evaluation. Methods References in the last decades at home and abroad about Senecio scandens clinical reports of adverse reactions were collated, analyzed and summarized. Results The most serious adverse reaction of Senecio scandens is hepatotoxicity, which is related to the long-term use, dosage, a mixture of varieties, compatibility, preparation technology and the difference of individual, and especially long-term use is the main cause of death of clinical cases. Studies have shown that pyrrolizidine alkaloids (PAs) contained in Senecio scandens are the main material basis of hepatotoxicity. Conclusion For the causes of Senecio scandens clinical adverse reactions, the hepatotoxicity damage regularity and material basis of Senecio scandens and its Chinese traditional patent medicine should be urgently studied, and intracorporal process of toxic substances and "toxicodynamics-toxicokinetics" relevance

  10. Ecología y distribución de Senecio pterophorus (Compositae en la Península Ibérica

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    Chamorro, Lourdes

    2006-06-01

    Full Text Available Field survey in Catalonia (Spain has increased the known distribution and the number of populations of the South African daisy Senecio pterophorus DC. in the Iberian Peninsula and mainland Europe. The species colonizes relatively disturbed habitats such as river beds, road borders and disturbed helm-oak communities with no limitation in light and water. The analysis of population structure related to habitat type has revealed that in riparian and ruderal areas populations are large and well-established, with a large number of members per age class. However, in disturbed forests populations have only a few scattered adults. Senecio pterophorus also shows great morphological plasticity related with habitat type: in open environments plants are shorter, adopt a spherical habit, and have smaller leaves than in forests, where they are taller and have leaves twice in size. This species may be considered as invasive in the Iberian Peninsula and mainland Europe, where it has rapidly spread in recent years. Furthermore, we suggest that S. pterophorus may be a threat to native species and habitat diversity as occurs in Australia, where the species displaces the native plants and hybridizes with some native Senecio species.En este trabajo se describen el área de distribución y el número de poblaciones de la especie sudafricana Senecio pterophorus DC. en Cataluña, única área de la Europa continental de donde hasta ahora se conoce. Esta especie coloniza ambientes perturbados, como lechos de ríos, márgenes de carreteras y áreas forestales perturbadas, siempre que no haya limitación por la luz y la humedad. En los hábitats riparios y ruderales las poblaciones son más estables y densas, con un gran número de individuos de diversas clases de edad, mientras que en ambientes forestales las poblaciones suelen contar con pocos individuos adultos distribuidos de forma dispersa. Senecio pterophorus presenta, además, una acusada plasticidad morfol

  11. Comparison of the anti-inflammatory active constituents and hepatotoxic pyrrolizidine alkaloids in two Senecio plants and their preparations by LC-UV and LC-MS.

    Science.gov (United States)

    Chen, Pinghong; Wang, Yi; Chen, Lulin; Jiang, Wei; Niu, Yan; Shao, Qing; Gao, Lu; Zhao, Quancheng; Yan, Licheng; Wang, Shufang

    2015-11-10

    Two Senecio plants, Senecio cannabifolius Less. and its variety S. cannabifolius Less. var. integrifolius (Kiodz.) Kidam., were both used as the raw material of Feining granule, a traditional Chinese medicine product for treating respiratory diseases. In this study, the chemical profiles of these two plants were investigated and compared by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR). A total number of 83 constituents, including 55 organic acids, 11 flavonoids, 4 alkaloids, 3 terpenes and 10 other types of compounds, were characterized. The results indicated that the levels of most flavonoids were higher in S. cannabifolius than in S. cannabifolius var. integrifolius, however, the levels of hepatotoxic pyrrolizidine alkaloids (PAs) were higher in S. cannabifolius var. integrifolius than in S. cannabifolius. Fifteen constituents were evaluated on lipopolysaccharides (LPS) induced RAW 264.7 cells, and eleven of them showed inhibition effect against nitric oxide (NO) production. Finally, the levels of ten major constituents (including seven anti-inflammatory active ones) and two PAs in Feining granule from two Senecio plants were determined and compared by the LC-UV and LC-MS methods, respectively. It was found that one organic acid (homogentisic acid) and two PAs (seneciphylline and senecionine) had higher contents in the preparation of S. cannabifolius var. integrifolius than in that of S. cannabifolius, however, the situations were inverse for the levels of four organic acids and flavonoids (chlorogenic acid, hyperoside, isoquercitrin, and isochlorogenic acid B). Based on the above results, S. cannabifolius might be a better raw material for Feining granule than S. cannabifolius var. integrifolius, because it contained more anti-inflammatory constituents and less hepatotoxic PAs than the latter. However, more pharmacological evaluations should be carried out to support the selection. The results in this study were helpful

  12. Pemphigus vulgaris in adolescence: a case report

    OpenAIRE

    Ferreira, Jean Paulo Rodolfo [UNESP; Araújo, Paula Caetano [UNESP; SAliba, Marcos Tadeu Adas [UNESP; Consolaro,Renata Bianco; Garbin,Cléa Adas Saliba

    2013-01-01

    Pemphigus vulgaris is an autoimmune disease, normally associated with oral lesions that generally affects patients aged 40 - 60 years. The aim of this study was to report a case of pemphigus vulgaris presenting at an uncommon age with advanced periodontal disease, and to describe the treatment performed from the time of diagnosis through oral environment restoration and follow-up monitoring. One gingival vesicular lesion was biopsied. After the histological confirmation of pemphigus vulgaris,...

  13. Extensive keloid formation after pemphigus vulgaris

    OpenAIRE

    Sako, Eric Y; Worswick, Scott

    2015-01-01

    Pemphigus vulgaris is an immunobullous disease characterized by intraepidermal blister formation. These blisters eventually rupture, leaving erosions that are slow to heal, often leaving hyperpigmented patches, but no scars. We describe a case of a 67- year-old man with pemphigus vulgaris who suffered severe keloidal scarring after the pemphigus lesions became infected. His keloids were treated with intralesional corticosteroids with some improvement. Pemphigus vulgaris, a process confined to...

  14. Extensive keloid formation after pemphigus vulgaris.

    Science.gov (United States)

    Sako, Eric Y; Workwick, Scott

    2015-11-18

    Pemphigus vulgaris is an immunobullous disease characterized by intraepidermal blister formation. These blisters eventually rupture, leaving erosions that are slow to heal, often leaving hyperpigmented patches, but no scars. We describe a case of a 67- year-old man with pemphigus vulgaris who suffered severe keloidal scarring after the pemphigus lesions became infected. His keloids were treated with intralesional corticosteroids with some improvement. Pemphigus vulgaris, a process confined to the epidermis, may lead to scarring in predisposed individuals, particularly if infection occurs.

  15. Variabilidad química de extractos y fracciones obtenidas de tres especies de la tribu Senecioneae: Gynoxys hirsuta, Pentacalia ledifolia, Senecio pampae

    OpenAIRE

    2011-01-01

    El estudio de la variabilidad química de extractos y fracciones de tres especies de la tribu Senecioneaede la familia Asteraceae se realizó con la intención de determinar los metabolitos secundarios principales de la tribu: alcaloides pirrolizidínicos, derivados de eremofilano, cumarinas y quinoles, en las plantas Gynoxys hirsuta, Pentacalia ledifolia y Senecio pampae con el fin de considerar a estos como marcadores quimiotaxonómicos de la tribu. Para la obtención de los extractos y fraccione...

  16. Therapeutic Agents in Acne Vulgaris

    Science.gov (United States)

    Stewart, Wm. D.; Maddin, Stuart; Nelson, A. J.; Danto, J. L.

    1963-01-01

    A total of 120 consecutive patients with pustular and cystic acne vulgaris were selected for study. Patients were assigned a placebo and a tetracycline medication in a random method. Of the 53 patients who were given tetracycline, 45 showed some response, which was fair in 19 and excellent in 26. Of the 55 patients who received placebo, 24 showed no response while 31 showed some improvement. No side effects were reported. The difference in response between the two groups is statistically significant. It is concluded that administration of 250 mg. tetracycline four times daily, even for periods as short as two weeks, enhances the likelihood of improvement of cystic or pustular acne vulgaris. PMID:14079132

  17. Aspek Imunologis pada Pemphigus Vulgaris

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    Saraventi Saraventi

    2015-10-01

    Full Text Available Pemphigus vulgaris is a relatively uncommon autoimmune disease marked with blister appearance of the skin tissue and mucosal membrane, resulting in loss of intercellular adhesion as well as the intact of epithelial cells, or so-called acantholysis. In this autoimmune disease the immune system forms autoantibodies attacking desmoglein, a specific protein at the skin tissue. The predisposing factors are presumed to include genetic and ethnic origin of the patient. Although at first usually without symptoms, after proceeding to the ulcerating stage the disease can result in fatal complications. Considering that almost 60% of the cases are first manifested in the oral mucosa, it is important that the dentists are aware of the basic etiology and immunology of the disease for correct diagnosis and therapy. This work reviews the etiopathogenesis of Pemphigus vulgaris, with emphasis on immunological aspects and immunotherapy.

  18. GLYCOSIDES FROM LINARIA VULGARIS MILL

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    Natalia Mashcenko

    2008-12-01

    Full Text Available A new flavonol glycoside, 5,4′-dimethylkaempferol 3-O-β-D-(6′′-α-Lrhamnopyranosyl -glucopyranoside, together with three known compounds were isolated from the n-butanolic soluble fraction of underground and aerial parts of Linaria vulgaris Mill, collected on the territory of Moldova. The characterisation of these compounds was achieved by various chromatographic and spectroscopic methods (IR, UV, 13C-NMR, 1H-NMR and MS.

  19. Intoxicação espontânea por Senecio brasiliensis (Asteraceae em ovinos no Rio Grande do Sul Spontaneous poisoning in sheep by Senecio brasiliensis (Asteraceae in southern Brazil

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    Marcia R. S. Ilha

    2001-09-01

    Full Text Available Descreve-se a ocorrência de um surto de intoxicação espontânea por Senecio brasiliensis em ovinos em um estabelecimento do município de Mata, Estado do Rio Grande do Sul, em meados de janeiro de 1997. De um total de 94 ovinos, 51 (54,25% animais adoeceram e 50 (53,2% morreram. Esse rebanho permaneceu durante aproximadamente 7 meses (de junho de 1996 a janeiro de 1997 em piquetes de pastagem nativa onde havia grande quantidade de S. brasiliensis. O quadro clínico manifestado pelos animais afetados consistia em fotossensibilização, emagrecimento progressivo, apatia, fraqueza, perturbações neurológicas como depressão, andar a esmo e desequilibrado, icterícia e hemoglobinúria. Houve melhora das lesões de pele naqueles ovinos que desenvolveram fotossensibilização hepatógena depois que foram retirados do sol. As principais lesões macroscópicas observadas em 9 dos 10 ovinos necropsiados incluíam fígado diminuído de tamanho, firme, difusamente marrom amarelado ou esverdeado, com quantidades variáveis de nódulos de 1-3 mm de diâmetro, bem circunscritos, salientes na cápsula, amarelados, distribuídos aleatoriamente por todo o parênquima. A vesícula biliar estava repleta e preenchida por bile verde escura e espessa. Havia também derrames cavitários (hidropericárdio e ascite. Crise hemolítica aguda fatal associada à intoxicação crônica hepatógena por cobre foi observada em cinco ovinos. Além das lesões hepáticas macroscópicas já mencionadas, foi observada icterícia generalizada da carcaça, rins tumefeitos, friáveis, difusamente escurecidos ou com fino pontilhado enegrecido; a urina era marrom escura (hemoglobinúria. As principais lesões microscópicas foram observadas no fígado e consistiam em hepatomegalocitose, proliferação de ductos biliares (hiperplasia ductal e fibrose periportal moderada acompanhada de infiltrado inflamatório mononuclear. Macrófagos carregados de pigmento acastanhado formavam

  20. Activity-guided isolation and identification of anti-staphylococcal components from Senecio tenuifolius Burm. F. leaf extracts

    Institute of Scientific and Technical Information of China (English)

    Manjunath Manubolu; Lavanya Goodla; Sivajyothi Ravilla; Vijayasarathi Reddy Obulum

    2013-01-01

    Objective: To investigate activity-guided isolation and identification of anti-Staphylococcus aures components from Senecio tenuifolius Burm. F. (S. tenuifolius). Methods: Hexane, chloroform, ethyl acetate, methanol and aqueous extracts of S. tenuifolius were prepared by soxilation for antimicrobial activity against one registered Staphylococcus aureus (S. aureus) (ATCC No: 25923) and two clinical isolates, methicillin resistant and methicillin sensitive S. aureus. NCCL standard methods were followed for antibacterial activity. GC-MS was performed to identify the chemical composition of bio active fraction. Results:Among all solvent extracts, methanol extract significantly reduced the growth of S. aureus (ATCC No: 25923), methicillin resistant and methicillin sensitive S. aureus with the best zone of inhibition at 16.23, 14.06 and 15.23 mm and minimum inhibition concentration (MIC) values at 426.16, 683.22 and 512.12 µg/mL, respectively. In order to detect the active component in methanol extract, it was further purified by column chromatography, which yielded four fractions (St1, St2, St3, and St4). Among these four fractions, St3 was effective against the tested strains of S. aures, with the best zone of inhibition at 15.09, 13.25 and 14.12 mm and with best MIC values at 88.16, 128.11 and 116.12 µg/mL, respectively. Effective fraction partially purified from S. tenuifolius (St3) yielded MIC’s that were at least 20 fold less when compared to crude extract. GC-MS analysis of St3 revealed the presence of 3-[methyl-6,7-dihydro benzofuran-4 (5H)-one], 1,2-benzenedicarboxylic acid, hydroquinone, methyl ester and 3 unknown compounds. Conclusions:The study provides scientific evidence for traditional and folklore medicinal use of S. tenuifolius in skin infections treatment.

  1. Plants growing on contaminated and brownfield sites appropriate for use in Organisation for Economic Co-operation and Development terrestrial plant growth test.

    Science.gov (United States)

    Sinnett, Danielle E; Lawrence, Victoria K; Hutchings, Tony R; Hodson, Mark E

    2011-01-01

    The Organisation for Economic Co-operation and Development (OECD) terrestrial plant test is often used for the ecological risk assessment of contaminated land. However, its origins in plant protection product testing mean that the species recommended in the OECD guidelines are unlikely to occur on contaminated land. Six alternative species were tested on contaminated soils from a former Zn smelter and a metal fragmentizer with elevated concentrations of Cd, Cu, Pb, and Zn. The response of the alternative species was compared with that of two species recommended by the OECD: Lolium perenne (perennial ryegrass) and Trifolium pratense (red clover). Urtica dioica (stinging nettle) and Poa annua (annual meadowgrass) had low emergence rates in the control soil and so may be considered unsuitable. Festuca rubra (Chewings fescue), Holcus lanatus (Yorkshire fog), Senecio vulgaris (common groundsel), and Verbascum thapsus (great mullein) offer good alternatives to the OECD species. In particular, H. lanatus and S. vulgaris were more sensitive to the soils with moderate concentrations of Cd, Cu, Pb, and Zn than the OECD species.

  2. PERFIL SOROLÓGICO EM EQUINOS INTOXICADOS EXPERIMENTALMENTE POR SENECIO BRASILIENSIS (COMPOSITAE. PARTE 1. DHL, AST, FAS e γGT

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    Sônia Terezinha dos Anjos Lopes

    1994-01-01

    Full Text Available RESUMO As partes aéreas dessecadas de Senecio brasiliensis, colhidas no período de brotação, foram administradas a 14 equinos de raça mista, com idade de 4 a 22 anos e pesando de 230 a 475kg. A planta era misturada à ração dada aos animais ou moída, misturada em água e administrada por sonda nasogástrica. Colheitas de sangue foram realizadas periodicamente em 14 equinos para avaliação da atividade enzimâtica sérica. Oito cavalos morreram em consequência da intoxicação. Três cavalos morreram por outras causas. Três cavalos sobreviveram sem apresentar quaisquer sinais clínicos durante o período em que foram observados. Nos oito equinos intoxicados, os sinais clínicos tiveram evolução de um a seis dias e caracterizaram-se por anorexia, icterícia e distúrbios neurológicos. Conclui-se que entre as provas laboratoriais usadas a gama-glutamiltransferase e aspartato-aminotransferase apresentaram significado prático melhor na avaliação da função hepática, pois a atividade sérica dessas enzimas estiveram elevadas em todos os equinos que desenvolveram a intoxicação por Senecio brasilieneis.

  3. Unrecognized dermatophyte infection in ichthyosis vulgaris.

    Science.gov (United States)

    Grahovac, Maja; Budimcić, Dragomir

    2009-01-01

    A case of unrecognized widespread dermatophyte infection associated with ichthyosis vulgaris and atopy is described. Our patient was a young woman in which the diagnosis of ichthyosis vulgaris and atopic dermatitis blocked the recognition of widespread dermatophyte infection for more than six months. The case showed some clinical peculiarities in terms of both extent of lesions and their clinical appearance.

  4. Sporotrichoid lupus vulgaris: A rare presentation.

    Science.gov (United States)

    Maheshwari, Anshul; Tiwari, Siddhi; Mathur, Deepak K; Bhargava, Puneet

    2015-01-01

    Lupus vulgaris is the most common presentation of cutaneous tuberculosis in India and can present as papular, nodular, plaque, ulcerative, vegetating, and tumid forms. Unusual variants include the frambesiform, gangrenous, ulcerovegetating, lichen simplex chronicus, myxomatous, and sporotrichoid types. We describe a rare sporotrichoid presentation of lupus vulgaris on the leg of a 28-year-old female of 12 years duration.

  5. Pemphigus vulgaris with solitary toxic thyroid nodule.

    Science.gov (United States)

    Alfishawy, Mostafa; Anwar, Karim; Elbendary, Amira; Daoud, Ahmed

    2014-01-01

    Background. Pemphigus vulgaris is an autoimmune vesiculobullous disease, affecting the skin and mucous membranes. It is reported to be associated with other autoimmune diseases including autoimmune thyroid diseases. However we report herein a case of pemphigus vulgaris associated with autonomous toxic nodule. Case Presentation. A 51-year-old woman was evaluated for blisters and erosions that develop on her trunk, face, and extremities, with a five-year history of progressively enlarging neck mass, and a past medical history of pemphigus vulgaris seven years ago. The condition was associated with palpitation, dyspnea, and heat intolerance. Thyroid function tests and thyroid scan were compatible with the diagnosis of thyrotoxicosis due to autonomous toxic nodule. Exacerbation of pemphigus vulgaris was proved by skin biopsy from the patient which revealed histologic picture of pemphigus vulgaris. Conclusion. Autoimmune thyroid diseases are reported to associate pemphigus vulgaris. To our knowledge, this case is the first in the English literature to report association between pemphigus vulgaris and autonomous toxic nodule and highlights the possibility of occurrence of pemphigus vulgaris with a nonautoimmune thyroid disease raising the question: is it just a coincidence or is there an explanation for the occurrence of both conditions together?

  6. Pemphigus Vulgaris with Solitary Toxic Thyroid Nodule

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    Mostafa Alfishawy

    2014-01-01

    Full Text Available Background. Pemphigus vulgaris is an autoimmune vesiculobullous disease, affecting the skin and mucous membranes. It is reported to be associated with other autoimmune diseases including autoimmune thyroid diseases. However we report herein a case of pemphigus vulgaris associated with autonomous toxic nodule. Case Presentation. A 51-year-old woman was evaluated for blisters and erosions that develop on her trunk, face, and extremities, with a five-year history of progressively enlarging neck mass, and a past medical history of pemphigus vulgaris seven years ago. The condition was associated with palpitation, dyspnea, and heat intolerance. Thyroid function tests and thyroid scan were compatible with the diagnosis of thyrotoxicosis due to autonomous toxic nodule. Exacerbation of pemphigus vulgaris was proved by skin biopsy from the patient which revealed histologic picture of pemphigus vulgaris. Conclusion. Autoimmune thyroid diseases are reported to associate pemphigus vulgaris. To our knowledge, this case is the first in the English literature to report association between pemphigus vulgaris and autonomous toxic nodule and highlights the possibility of occurrence of pemphigus vulgaris with a nonautoimmune thyroid disease raising the question: is it just a coincidence or is there an explanation for the occurrence of both conditions together?

  7. Lupus vulgaris with squamous cell carcinoma.

    Science.gov (United States)

    Motswaledi, Mojakgomo Hendrick; Doman, Chantal

    2007-12-01

    Tuberculosis is still a significant problem in developing countries. Cutaneous forms of tuberculosis account for approximately 10% of all cases of extrapulmonary tuberculosis. Cutaneous tuberculosis may be because of true infection with Mycobacterium tuberculosis or because of tuberculids. Tuberculids are immunological reactions to haematogenously spread antigenic components of M. tuberculosis. True cutaneous tuberculosis may be because of inoculation or haematogenous spread of M. tuberculosis to the skin. Lupus vulgaris is the commonest form of true cutaneous tuberculosis. Other forms of true cutaneous tuberculosis are tuberculous chancre, tuberculosis verrucosa cutis, scrofuloderma, periorificial tuberculosis and miliary tuberculosis of the skin. Lupus vulgaris is usually chronic and progressive. It occurs in patients with moderate to high immunity against M. tuberculosis as evidenced by strongly positive tuberculin test. Long-standing cases of lupus vulgaris may be complicated by squamous cell carcinoma (SCC). We describe a patient who had undiagnosed lupus vulgaris for 35 years until she developed SCC on the lesion of lupus vulgaris.

  8. Pseudoainhum associated with Psoriasis vulgaris

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    Mrinal Gupta

    2015-10-01

    Full Text Available Pseudoainhum is the term applied to constricting bands around the digits or the limb which are either congenital or secondary to another disease. Progression of the constriction bands can lead to irreversible damage and autoamputation of the affected digit. Congenital pseudoainhum usually results due to amniotic bands or adhesions in utero while acquired pseudoainhum is usually secondary to trauma, neuropathy, systemic sclerosis or infections like leprosy. Psoriasis is a rare cause of acquired pseudoainhum with only five cases reported till date. We report a case of pseudoainhum secondary to psoriasis vulgaris in a 45 year old male which was successfully treated with topical corticosteroids and systemic methotrexate therapy.

  9. Lupus vulgaris of the auricle

    Directory of Open Access Journals (Sweden)

    Hojat Eftekhari

    2017-02-01

    Full Text Available Nowadays despite decrease in the tuberculosis (TB incidence around the world with standard anti tubercular treatment, atypical forms of TB is increasing due to extensive use of immunosuppressive therapy for autoimmune diseases and increasing prevalence of HIV infection, so early diagnosis of pulmonary and extra pulmonary TB in these patients is very important. Cutaneous TB could be a great imitator and confused with other granulomatous lesions. It has different morphological patterns. Lupus vulgaris (LV is the most common type of cutaneous TB which usually involves head and neck. We present herein a case of LV in auricular region without apparent systemic involvement.

  10. Vegetative propagation of Bambusa vulgaris

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    Rafael Malfitano Braga

    2017-06-01

    Full Text Available Bamboo is an important source of raw material of multiple uses. The development of simple techniques for its propagation is a practical way to enable its implementation in ownership of low technology. The present work had the objective of evaluating artisanal propagation methods for Bambusa vulgaris. Two types of propagules were tested, with buds budded or not, and three relative positions to the removal of vegetative material on the culm. The best propagule was with only one node, extracted from the lower thirds of the stem, presenting 72% of rooting. This result demonstrates its potential for seedling production of this species under low tech.

  11. Management strategies for acne vulgaris

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    Whitney KM

    2011-04-01

    Full Text Available Kristen M Whitney1, Chérie M Ditre21Philadelphia College of Osteopathic Medicine, Philadelphia, PA, USA; 2Skin Enhancement Center and Cosmetic Dermatology, Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, PA, USADate of preparation: 30th November 2010Conflicts of interest: None declaredClinical question: What are the most effective treatment(s for mild, moderate, severe, and hormonally driven acne?Results: Mild acne responds favorably to topical treatments such as benzoyl peroxide, salicylic acid, and a low-dose retinoid. Moderate acne responds well to combination therapy comprising-topical benzoyl peroxide, antibiotics, and/or retinoids, as well as oral antibiotics in refractory cases and oral contraceptive pills for female acne patients. Severe nodulocystic acne vulgaris responds best to oral isotretinoin therapy. In female patients with moderate to severe acne, facial hair, loss of scalp hair and irregular periods, polycystic ovarian syndrome should be considered and appropriate treatment with hormonal modulation given. Adjunctive procedures can also be considered for all acne patients.Implementation: Pitfalls to avoid when treating acne: treatment of acne in women of childbearing age; familiarization of all acne treatments in order to individualize management for patients; indications for specialist referral.Keywords: acne vulgaris, benzoyl peroxide, retinoids, antibiotics, light and laser therapy, photodynamic therapy, photopneumatic therapy, chemical peels

  12. PATIENT WITH VERUCCA VULGARIS RECCURENT

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    Ni Made Gita Saraswati

    2013-05-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 The common wart or verruca vulgaris are benign lesions, elevated, firm nodules with characteristic papillomatous surface projections. This lession could appear anywhere in the body, mostly in fingers. The lesion could spread anywhere in the body, leaving a scar and also have a high incidence of reccurency. This report describe a man with verruca vulgaris recurrent. Preventive is important to prevent the reccurency, beside electrodesication as a curative therapy. /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

  13. Hypertrophic lupus vulgaris: an unusual presentation.

    Science.gov (United States)

    Jain, Vijay K; Aggarwal, Kamal; Jain, Sarika; Singh, Sunita

    2009-07-01

    Lupus vulgaris is the most common form of cutaneous tuberculosis occurring in previously sensitized individuals with a high degree of tuberculin sensitivity. Various forms including plaque, ulcerative, hypertrophic, vegetative, papular, and nodular forms have been described. A 30-year-old male patient presented with a very large hypertrophic lupus vulgaris lesion over left side of chest since 22 years. Histopathological examination showed granulomatous infiltration without caseation necrosis. The Mantoux reaction was strongly positive. Hypertrophic lupus vulgaris of such a giant size and that too at an unusual site is extremely rare and hence is being reported.

  14. Extensive keloidal healing of pemphigus vulgaris

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    Khanna Neena

    1997-01-01

    Full Text Available Bullae of pemphigus vulgaris heal without scarring. We here report a patient of pemphigus vulgaris whose lesions healed with a one-month history of extensive flaccid bullae and uninfected erosions on the trunk and extremities along with superficial erosions in the oral mucosa. The clinical suspicion of pemphigus vulgaris was confirmed by histopathological and immunohistological examination. Pulse therapy with monthly parenteral dexamethasone and cyclophosphamide pulse was instituted. The cutaneous lesions on healing formed extensive keloidal scars despite high dose of monthly corticosteroid therapy.

  15. Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2 infecting common bean Phaseolus vulgaris genotypes show differential infection patterns between gene pools

    Science.gov (United States)

    We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 (PvEV-1) and Phaseolus vulgaris endornavirus 2 (PvEV-2), in breeding-lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) as well as other Phaseolus species collected from two...

  16. Berberis Vulgaris and Berberine: An Update Review.

    Science.gov (United States)

    Imenshahidi, Mohsen; Hosseinzadeh, Hossein

    2016-11-01

    Berberine is an isoquinoline alkaloid present in several plants, including Coptis sp. and Berberis sp. Berberine is a customary component in Chinese medicine, and is characterized by a diversity of pharmacological effects. An extensive search in electronic databases (PubMed, Scopus, Ovid, Wiley, ProQuest, ISI, and Science Direct) were used to identify the pharmacological and clinical studies on Berberis vulgaris and berberine, during 2008 to 2015, using 'berberine' and 'Berberis vulgaris' as search words. We found more than 1200 new article studying the properties and clinical uses of berberine and B. vulgaris, for treating tumor, diabetes, cardiovascular disease, hyperlipidemia, inflammation, bacterial and viral infections, cerebral ischemia trauma, mental disease, Alzheimer disease, osteoporosis, and so on. In this article, we have updated the pharmacological effects of B. vulgaris and its active constituent, berberine. Copyright © 2016 John Wiley & Sons, Ltd.

  17. Pulse Clarithromycin Therapy In Severe ACNE Vulgaris

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    Rathi Sanjay K

    2002-01-01

    Full Text Available Three patients with severe acne vulgaris, not responding with long courses of doxycycline, minocycline and erythromycin were given oral clarithromycin in pulsed regimen. The patients were given 7 days course of clarithromycin 250mg twice daily, which was repeated after a gap of 10 days. Such 3 courses were given. The lesions responded significantly. No significant side effect was noted. Pulse clarithromycin therapy seems to be a good alternative and effective tool in the management of severe acne vulgaris.

  18. Neonatal and infantile acne vulgaris: an update.

    Science.gov (United States)

    Serna-Tamayo, Cristian; Janniger, Camila K; Micali, Giuseppe; Schwartz, Robert A

    2014-07-01

    Acne may present in neonates, infants, and small children. Neonatal and infantile acne vulgaris are not considered to be rare. The presentation of acne in this patient population sometimes represents virilization and may portend later development of severe adolescent acne. Neonatal and infantile acne vulgaris must be distinguished from other cutaneous disorders seen in newborns and infants. Infantile acne tends to be more pleomorphic and inflammatory, thus requiring more vigorous therapy than neonatal acne.

  19. Pemphigus vulgaris presenting with multiple lesion morphologies.

    Science.gov (United States)

    Song, Philip In; Divito, Sherrie J; Kroshinsky, Daniela

    2014-12-14

    Pemphigus vulgaris is an uncommon intraepidermal blistering disorder that typically presents with flaccid bullae or erosions. We report a patient with pemphigus vulgaris who presented with several unusual clinical features: tense bullae with dependently layered pus, true target lesions coalescing into annular configurations, and diffuse desquamation that initially raised concern for toxic epidermal necrolysis. We discuss the differential diagnosis and implications of these morphological findings.

  20. Management of pemphigus vulgaris: challenges and solutions

    OpenAIRE

    Gregoriou S; Efthymiou O; Stefanaki C; Rigopoulos D

    2015-01-01

    Stamatis Gregoriou, Ourania Efthymiou, Christina Stefanaki, Dimitris Rigopoulos 2nd Department of Dermatology and Venereology, University of Athens Medical School, Attikon Hospital, Athens, Greece Abstract: The main objective in the treatment of pemphigus vulgaris is to control the disease, prevent relapses, and avoid adverse events associated with the prolonged use of steroids and immunosuppressive agents. Systemic corticosteroids remain the gold standard treatment for pemphigus vulgaris. A...

  1. Response of Desulfovibrio vulgaris to Alkaline Stress

    Energy Technology Data Exchange (ETDEWEB)

    Stolyar, S.; He, Q.; He, Z.; Yang, Z.; Borglin, S.E.; Joyner, D.; Huang, K.; Alm, E.; Hazen, T.C.; Zhou, J.; Wall, J.D.; Arkin, A.P.; Stahl, D.A.

    2007-11-30

    The response of exponentially growing Desulfovibrio vulgarisHildenborough to pH 10 stress was studied using oligonucleotidemicroarrays and a study set of mutants with genes suggested by microarraydata to be involved in the alkaline stress response deleted. The datashowed that the response of D. vulgaris to increased pH is generallysimilar to that of Escherichia coli but is apparently controlled byunique regulatory circuits since the alternative sigma factors (sigma Sand sigma E) contributing to this stress response in E. coli appear to beabsent in D. vulgaris. Genes previously reported to be up-regulated in E.coli were up-regulated in D. vulgaris; these genes included three ATPasegenes and a tryptophan synthase gene. Transcription of chaperone andprotease genes (encoding ATP-dependent Clp and La proteases and DnaK) wasalso elevated in D. vulgaris. As in E. coli, genes involved in flagellumsynthesis were down-regulated. The transcriptional data also identifiedregulators, distinct from sigma S and sigma E, that are likely part of aD. vulgaris Hildenborough-specific stress response system.Characterization of a study set of mutants with genes implicated inalkaline stress response deleted confirmed that there was protectiveinvolvement of the sodium/proton antiporter NhaC-2, tryptophanase A, andtwo putative regulators/histidine kinases (DVU0331 andDVU2580).

  2. Serum angiotensin converting enzyme in pemphigus vulgaris

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    Reza M Robati

    2014-01-01

    Full Text Available Background: Pemphigus vulgaris is an autoimmune blistering skin disease with unknown etiology. Drugs such as angiotensin-converting enzyme (ACE inhibitors may contribute in the pathogenesis of pemphigus. Objective: We plan this essay to evaluate the serum ACE level in pemphigus vulgaris patients in comparison with healthy controls to recognize its possible role in disease pathogenesis or activity. Methods: This study was planned and performed in the dermatology clinics of Shahid Beheshti University of MedicalSciences′ Hospitals between July 2010 and June 2011. Patients with new onset of pemphigus vulgaris were enrolled in our study. Control subjects were frequency-matched to cases by sex and age. Serum ACE was determined by the spectrophotometric method. Results: Thirty-four patients with pemphigus vulgaris and 35 healthy individuals were recruited in the study. No statistical significant difference was detected in the mean level of serum ACE of the two groups (t-test, P = 0.11. The mean ACE level was significantly lower in male patients compared with male controls (P = 0.04. Moreover, a significant higher serum ACE level of patients with cutaneous involvement was observed compared to patients with mucosal involvement (P = 0.02. Conclusions: Despite lack of any significant difference of serum ACE level between pemphigus and control group, the serum ACE level was considerably lower in male pemphigus vulgaris patients compared with male controls. Therefore, ACE might have some association with pemphigus vulgaris especially in male patients; however, further studies are required to confirm this association.

  3. Pemphigus vulgaris: approach to treatment.

    Science.gov (United States)

    Sinha, Animesh A; Hoffman, Melissa B; Janicke, Elise C

    2015-04-01

    The therapeutic management of pemphigus vulgaris (PV) is centered around immunosuppression, which can be generalized, as in the use of corticosteroids or steroid sparing agents, or specific, as in therapeutic blockage of autoantibody production, certain cytokines, or signaling pathways. Currently, the backbone of treatment for PV, particularly, first line therapy, remains systemic corticosteroids. Although very effective, the significant side effects of long-term corticosteroid usage are well documented. Adjunctive therapies aim to eliminate, or at least decrease, the necessary dose of corticosteroids. Specifically, azathioprine, cyclophosphamide, methotrexate, cyclosporine, mycophenolate mofetil and dapsone are now widely used in PV. Intravenous immunoglobulin (IVIG), plasmapheresis, immunoadsorption, and most recently, rituximab, are other members of the therapeutic armamentarium. However, despite the widening range of treatment options in PV, well-controlled clinical trials and consensus guidelines are lacking.

  4. Sunflower Seed and Acne Vulgaris.

    Science.gov (United States)

    Mohebbipour, Alireza; Sadeghi-Bazargani, Homayoun; Mansouri, Mona

    2015-09-01

    Regardless of the overall association between diet and acne which cannot be easily ignored, there might be an association between specific nutrients and acne development or improvement. The aim of this study was to assess the effect of dietary intake of sunflower seeds on acne severity and the pattern of acne lesions. In a randomized controlled trial, 50 patients aged 15 - 30 years old with acne vulgaris were enrolled through consecutive convenient sampling, in a dermatology clinic in Ardabil, Iran. They were randomly allocated into two trial arms. Those in the control group were asked to stop eating sunflower seeds if they did before. In the intervention group, they consumed 25 g sunflower-containing food daily for seven days. The primary outcome of interest was 10% increase/decrease in the baseline acne severity index (ASI), sustained to the end of the follow-up period on day 14. The mean ASI did not change significantly through the study period in the control group, but it increased in the sunflower group from 62 at the baseline to 86.8 after two weeks (P acne grading score (GAGS) did not significantly change in any of the groups and the difference in the change of GAGS was not significant between the groups (2.4 in the sunflower group versus 1.6 in the control group). Twenty two subjects (88%) in the sunflower group versus 9 (36%) in the control group had at least 10% increment in ASI throughout the follow-up period (P acne vulgaris; however, further evidence is needed to ban sunflower seed intake in patients with acne. Considering the observed potential negative effect in this trial, future randomized clinical trials may base their design on randomly assigning the exposed patients to give up use of sunflower seed intake.

  5. PEMANFAATAN MIKROALGA LAUT Chlorella vulgaris SUMBER DHA DAN EPA

    OpenAIRE

    2016-01-01

    Penelitian tentang mikroalga laut jenis Chlorella vulgaris telah dilakukan. Chlorella vulgaris dipilih sebagai bahan penambah gizi untuk di fortifikasi kedalam makanan . Penelitian ini bertujuan untuk mengetahui kandungan gizi dengan menganalisis kandungan DHA dan EPA. Penelitian ini dilakukan dengan mengkultur fitoplankton Chlorella vulgaris dan dipanen setelah media kultur mencapai fase Stasioner. Kemudian, dikeringkan dengan menggunakan freeze dryer, biomassa kering dianalisis kandungan DH...

  6. Complementary therapies for acne vulgaris.

    Science.gov (United States)

    Cao, Huijuan; Yang, Guoyan; Wang, Yuyi; Liu, Jian Ping; Smith, Caroline A; Luo, Hui; Liu, Yueming

    2015-01-19

    Acne is a chronic skin disease characterised by inflamed spots and blackheads on the face, neck, back, and chest. Cysts and scarring can also occur, especially in more severe disease. People with acne often turn to complementary and alternative medicine (CAM), such as herbal medicine, acupuncture, and dietary modifications, because of their concerns about the adverse effects of conventional medicines. However, evidence for CAM therapies has not been systematically assessed. To assess the effects and safety of any complementary therapies in people with acne vulgaris. We searched the following databases from inception up to 22 January 2014: the Cochrane Skin Group Specialised Register, the Cochrane Central Register of Controlled Trials (CENTRAL; 2014,Issue 1), MEDLINE (from 1946), Embase (from 1974), PsycINFO (from 1806), AMED (from 1985), CINAHL (from 1981), Scopus (from 1966), and a number of other databases listed in the Methods section of the review. The Cochrane CAM Field Specialised Register was searched up to May 2014. We also searched five trials registers and checked the reference lists of articles for further references to relevant trials. We included parallel-group randomised controlled trials (or the first phase data of randomised cross-over trials) of any kind of CAM, compared with no treatment, placebo, or other active therapies, in people with a diagnosis of acne vulgaris. Three authors collected data from each included trial and evaluated the methodological quality independently. They resolved disagreements by discussion and, as needed, arbitration by another author. We included 35 studies, with a total of 3227 participants. We evaluated the majority as having unclear risk of selection, attrition, reporting, detection, and other biases. Because of the clinical heterogeneity between trials and the incomplete data reporting, we could only include four trials in two meta-analyses, with two trials in each meta-analysis. The categories of CAM included

  7. Ocular involvement in pemphigus vulgaris.

    Science.gov (United States)

    Akhyani, Maryam; Keshtkar-Jafari, Alireza; Chams-Davatchi, Cheyda; Lajevardi, Vahide; Beigi, Sara; Aghazadeh, Nessa; Rayati Damavandi, Maede; Arami, Shabnam

    2014-07-01

    Pemphigus vulgaris (PV) is an autoimmune disorder affecting the skin and mucous membranes. Ocular involvement in PV has been reported but its prevalence and clinical characteristics are not well defined. This prospective cross-sectional study of 103 PV patients was designed to determine the prevalence, clinical types and epidemiological trends of ocular involvement in a population of Iranian patients with PV. Ocular involvement was present in 17 (16.5%) patients. Conjunctivitis was the most prevalent type of ocular involvement (9/17, 52.9%), followed by erosion of the palpebral conjunctiva (7/17, 41.2%). Erosion of the bulbar conjunctiva was noted in only one patient (5.9%). The most commonly reported symptoms were eye irritation (76.5%) and redness (76.5%). No significant relation was found between ocular involvement and disease activity (partial remission or relapse). Mucoid discharge was significantly more common in patients with conjunctival erosions as compared to patients with conjunctivitis (P = 0.038). We conclude that ocular involvement is not rare in PV; 16.5% of PV patients develop ocular disease independent of the disease activity and extension. Conjunctivitis is the most common type of involvement, however, palpebral conjunctival erosion is more frequent than previously realized.

  8. Twins with neonatal pemphigus vulgaris born to a mother with pemphigus vulgaris: a case report.

    Science.gov (United States)

    Itsukaichi, Mina; Takakuwa, Koichi; Yamaguchi, Masayuki; Serikawa, Takehiro; Tanaka, Kenichi; Kojima, Kinuko; Sakakibara, Seiichi; Usuda, Tohei; Matsunaga, Masamichi; Hashimoto, Tsuyoshi

    2013-01-01

    Dichorionic diamniotic twins were born at 37 weeks of gestation by cesarean section to a 34-year-old primigravid Japanese woman because the first twin was in breech presentation. The mother had been diagnosed with pemphigus vulgaris prior to her pregnancy. In addition to a high antidesmoglein 3 autoantibody titer, flaccid bullae and erosions on both of the twins' lips and in their oral cavities at 13 days of age led to the diagnosis of neonatal pemphigus vulgaris. This case highlights the need for awareness that pemphigus vulgaris may not occur immediately after birth.

  9. The adaptive genome of Desulfovibrio vulgaris Hildenborough.

    Science.gov (United States)

    Santana, Margarida; Crasnier-Mednansky, Martine

    2006-07-01

    Peculiar attributes revealed by sequencing the genome of Desulfovibrio vulgaris Hildenborough are analyzed, particularly in relation to the presence of a phosphotransferase system (PTS). The PTS is a typical bacterial carbohydrate transport system functioning via group translocation. Novel avenues for investigations are proposed emphasizing the metabolic diversity of D. vulgaris Hildenborough, especially the likely utilization of mannose-type sugars. Comparative analysis with PTS from other Gram-negative and Gram-positive bacteria indicates regulatory functions for the PTS of D. vulgaris Hildenborough, including catabolite repression and inducer exclusion. Chemotaxis towards PTS substrates is considered. Evidence suggests that this organism may not be a strict anaerobic sulfate reducer typical of the ocean, but a versatile organism capable of bidirectional transmigration and adaptation to both water and terrestrial environments.

  10. THERAPEUTIC AGENTS IN ACNE VULGARIS. I. TETRACYCLINE.

    Science.gov (United States)

    STEWART, W D; MADDIN, S; NELSON, A J; DANTO, J L

    1963-11-23

    A total of 120 consecutive patients with pustular and cystic acne vulgaris were selected for study. Patients were assigned a placebo and a tetracycline medication in a random method. Of the 53 patients who were given tetracycline, 45 showed some response, which was fair in 19 and excellent in 26. Of the 55 patients who received placebo, 24 showed no response while 31 showed some improvement. No side effects were reported. The difference in response between the two groups is statistically significant. It is concluded that administration of 250 mg. tetracycline four times daily, even for periods as short as two weeks, enhances the likelihood of improvement of cystic or pustular acne vulgaris.

  11. Aqueous extract of Senecio candicans DC induce liver and kidney damage in a sub-chronic oral toxicity study in Wistar rats.

    Science.gov (United States)

    Lakshmanan, Hariprasath; Raman, Jegadeesh; Pandian, Arjun; Kuppamuthu, Kumaresan; Nanjian, Raaman; Sabaratam, Vikineswary; Naidu, Murali

    2016-08-01

    Senecio candicans DC. (Asteraceae) is used as a remedy for gastric ulcer and stomach pain in the Nilgiris, district, Tamil Nadu. The present investigation was carried out to evaluate the sub-chronic toxicity of an aqueous extract of Senecio candicans (AESC) plant in Wistar albino rats. The study was conducted in consideration of the OECD 408 study design (Repeated Dose 90-Day Oral Toxicity Study in Rodents) and the extract was administered via gavage at doses of 250, 500 or 750 mg/kg body weight per day for 90-days. Hematological, biochemical parameters were determined on days 0, 30, 60 and 90 of administration. Animals were euthanized after 90 d treatment and its liver and kidney sections were taken for histological study. The results of sub-chronic study showed significant increase (P < 0.05) in serum uric acid, creatinine, aspartate transaminase (AST) and alanine transaminase (ALP) levels. Histological examination of liver showed mild mononuclear infiltration in the portal trait, enlarged nucleus around the central vein and mild loss of hepatocyte architecture in rats treated with 750 mg/kg of AESC. Histological examination of kidney showed focal interstitial fibrosis, crowding of glomeruli and mild hydropic change with hypercellular glomeruli in rats treated with 750 mg/kg of AESC. However, no remarkable histoarchitectural change in hepatocytes and glomeruli were observed in rats treated with lower concentrations (250 and 500 mg/kg b.w.) of AESC compared to control group animals. The no-observed adverse effect level (NOAEL) of AESC in the present study was 500 mg/kg b.w. Signs of toxic effects are evident from the current study. Although AESC contains low concentrations of PA, findings from this study suggest that regular consumers of herbal remedies derived from this plant may develop kidney and liver toxicity. Further studies on the isolation and characterization of PAs are necessary to determine the safe dose level of the extract for therapeutic use

  12. 峨眉千里光防控药剂的筛选及防治效果%Screening and Efficacy of Herbicides against Senecio faberi

    Institute of Scientific and Technical Information of China (English)

    余慧灵; 王学贵; 田孟良; 李文光; 袁勇

    2012-01-01

    采用盆栽法评价了12种除草剂对峨眉千里光(Senecio faberii Hemsl)的防除效果及同一生境中非靶标杂草的安全性.结果表明,56%2甲4氯钠可溶性粉剂、48%麦草畏水剂、25%氟磺胺草醚水剂、30%二氯吡啶酸水剂、48%三氯吡氧乙酸乳油效果较好.进一步研究了该5种除草剂的田间防治效果,结果表明,30%二氯吡啶酸水剂和48%三氯吡氧乙酸乳油表现出很好的株防效和鲜重防效,对同一生境中其他非靶标杂草无影响.因此,该两种药剂可有效防除峨眉千里光,控制其危害和蔓延.%The efficacies of 12 herbicides on Senecio faberi Hemsl. and safety on non-target weeds in the same environment were evaluated with pot-culture method. The results suggested that 56% MCPA-Na soluble powder, 48% dicamba aqueous solution agent, 25% fomesafen aqueous solution agent, 30% clopyralid aqueous solution agent, 48% triclopyr emulsifiable solution had better efficacy than the other tested herbicides. The efficacy of the five herbicides on S. faberi Hemsl. and safety on non-target weeds in the field were further studied and found that 30% clopyralid aqueous solution agent and 48% triclopyr emulsifiable solution displayed good stem control and fresh weight reductions efficacy. What's more, no damages on non-target weeds in the same environment was observed during the investigation period. So the two herbicides could be effective in controlling the hazard and extension of S. faberi.

  13. Photodynamic therapy of acne vulgaris.

    Science.gov (United States)

    Ershova, Ekaterina Y.; Karimova, Lubov N.; Kharnas, Sergey S.; Kuzmin, Sergey G.; Loschenov, Victor B.

    2003-06-01

    Photodynamic therapy (PDT) with topical 5-aminolevulinic acid (ALA) was tested for the treatment of acne vulgaris. Patients with acne were treated with ALA plus red light. Ten percent water solution of ALA was applied with 1,5-2 h occlusion and then 18-45 J/cm2 630 nm light was given. Bacterial endogenous porphyrins fluorescence also was used for acne therapy. Treatment control and diagnostics was realized by fluorescence spectra and fluorescence image. Light sources and diagnostic systems were used: semiconductor laser (λ=630 nm, Pmax=1W), (LPhT-630-01-BIOSPEC); LED system for PDT and diagnostics with fluorescent imager (λ=635 nm, P=2W, p=50 mW/cm2), (UFPh-630-01-BIOSPEC); high sensitivity CCD video camera with narrow-band wavelength filter (central wavelength 630 nm); laser electronic spectrum analyzer for fluorescent diagnostics and photodynamic therapy monitoring (LESA-01-BIOSPEC). Protoporphyrin IX (PP IX) and endogenous porphyrins concentrations were measured by fluorescence at wavelength, correspondingly, 700 nm and 650 nm. It was shown that topical ALA is converted into PP IX in hair follicles, sebaceous glands and acne scars. The amount of resulting PP IX is sufficient for effective PDT. There was good clinical response and considerable clearance of acne lesion. ALA-PDT also had good cosmetic effect in treatment acne scars. PDT with ALA and red light assist in opening corked pores, destroying Propionibacterium acnes and decreasing sebum secretion. PDT treatment associated with several adverse effects: oedema and/or erytema for 3-5 days after PDT, epidermal exfoliation from 5th to 10th day and slight pigmentation during 1 month after PDT. ALA-PDT is effective for acne and can be used despite several side effects.

  14. Light attenuation on Chlorella vulgaris cells

    Science.gov (United States)

    Krol, Tadeusz; Lotocka, Maria

    1993-12-01

    The laboratory measurements of spectrum of light attenuation on phytoplankton particles i.e. monoculture of unicellural green algae Chlorella vulgaris are presented. The measurements were carried out for alive culture and the cultures subjected to chemical (NaOH) or physical (ultrasounds) modification. The distinct changes in the light attenuation spectrum were a result of modification of the internal cell structures.

  15. Primary structure of hemocyanin from Palinurus vulgaris

    NARCIS (Netherlands)

    Jekel, PA; Neuteboom, B; Beintema, JJ

    1996-01-01

    The primary structure of hemocyanin from the spiny lobster Palinurus vulgaris was determined using a mixture of at least four slightly different subunits. Heterogeneities were observed in 32 (5%) of the positions. The amino acid sequence differs at about 20% of the positions from that of subunit a o

  16. Chlorotic mottle of bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Jayasinghe, W.U.

    1982-01-01

    For the past years there have been outbreaks of a disease of bean ( Phaseolus vulgaris L.) in Colombia called bean chlorotic mottle. The etiology of bean chlorotic mottle was not known, but the disease was generally believed to be incited by the same whitefly-transmitted virus that causes variegatio

  17. Ichthyosis vulgaris: the filaggrin mutation disease.

    Science.gov (United States)

    Thyssen, J P; Godoy-Gijon, E; Elias, P M

    2013-06-01

    Ichthyosis vulgaris is caused by loss-of-function mutations in the filaggrin gene (FLG) and is characterized clinically by xerosis, scaling, keratosis pilaris, palmar and plantar hyperlinearity, and a strong association with atopic disorders. According to the published studies presented in this review article, FLG mutations are observed in approximately 7·7% of Europeans and 3·0% of Asians, but appear to be infrequent in darker-skinned populations. This clinical review article provides an overview of ichthyosis vulgaris epidemiology, related disorders and pathomechanisms. Not only does ichthyosis vulgaris possess a wide clinical spectrum, recent studies suggest that carriers of FLG mutations may have a generally altered risk of developing common diseases, even beyond atopic disorders. Mechanistic studies have shown increased penetration of allergens and chemicals in filaggrin-deficient skin, and epidemiological studies have found higher levels of hand eczema, irritant contact dermatitis, nickel sensitization and serum vitamin D levels. When relevant, individuals should be informed about an increased risk of developing dermatitis when repeatedly or continuously exposed to nickel or irritants. Moreover, with our current knowledge, individuals with ichthyosis vulgaris should be protected against neonatal exposure to cats to prevent atopic dermatitis and should abstain from smoking to prevent asthma. Finally, they should be advised against excessive exposure to factors that decrease skin barrier functions and increase the risk of atopic dermatitis.

  18. Chlorotic mottle of bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Jayasinghe, W.U.

    1982-01-01

    For the past years there have been outbreaks of a disease of bean ( Phaseolus vulgaris L.) in Colombia called bean chlorotic mottle. The etiology of bean chlorotic mottle was not known, but the disease was generally believed to be incited by the same whitefly-transmitted virus

  19. Filipendula vulgaris Moench teruggevonden bij Gennep

    NARCIS (Netherlands)

    Westhoff, V.; Leeuwen, v. Chr.G.

    1957-01-01

    De in Nederland als enig bekende groeiplaats van Filipendula vulgaris Moench (F. hexapetala Gilib.) nabij het Sanatorium “Zonlichtheide” te Gennep, is enkele jaren geleden ten offer gevallen aan de zandwinning, die daar in de Maasuiterwaard heeft plaatsgevonden. Onze vreugde was dan ook groot toen w

  20. Ecological differentiation, lack of hybrids involving diploids, and asymmetric gene flow between polyploids in narrow contact zones of Senecio carniolicus (syn. Jacobaea carniolica, Asteraceae).

    Science.gov (United States)

    Hülber, Karl; Sonnleitner, Michaela; Suda, Jan; Krejčíková, Jana; Schönswetter, Peter; Schneeweiss, Gerald M; Winkler, Manuela

    2015-03-01

    Areas of immediate contact of different cytotypes offer a unique opportunity to study evolutionary dynamics within heteroploid species and to assess isolation mechanisms governing coexistence of cytotypes of different ploidy. The degree of reproductive isolation of cytotypes, that is, the frequency of heteroploid crosses and subsequent formation of viable and (partly) fertile hybrids, plays a crucial role for the long-term integrity of lineages in contact zones. Here, we assessed fine-scale distribution, spatial clustering, and ecological niches as well as patterns of gene flow in parental and hybrid cytotypes in zones of immediate contact of di-, tetra-, and hexaploid Senecio carniolicus (Asteraceae) in the Eastern Alps. Cytotypes were spatially separated also at the investigated microscale; the strongest spatial separation was observed for the fully interfertile tetra- and hexaploids. The three main cytotypes showed highly significant niche differences, which were, however, weaker than across their entire distribution ranges in the Eastern Alps. Individuals with intermediate ploidy levels were found neither in the diploid/tetraploid nor in the diploid/hexaploid contact zones indicating strong reproductive barriers. In contrast, pentaploid individuals were frequent in the tetraploid/hexaploid contact zone, albeit limited to a narrow strip in the immediate contact zone of their parental cytotypes. AFLP fingerprinting data revealed introgressive gene flow mediated by pentaploid hybrids from tetra- to hexaploid individuals, but not vice versa. The ecological niche of pentaploids differed significantly from that of tetraploids but not from hexaploids.

  1. The influence of light spectra, UV-A, and growth regulators on the in vitro seed germination of Senecio cineraria DC.

    Directory of Open Access Journals (Sweden)

    Cristiane Pimentel Victório

    2010-10-01

    Full Text Available This study was carried out to investigate the effects of light spectra, additional UV-A, and different growth regulators on the in vitro germination of Senecio cineraria DC. Seeds were surface-sterilized and inoculated in MS medium to evaluate the following light spectra: white, white plus UV-A, blue, green, red or darkness. The maximum germinability was obtained using MS0 medium under white light (30% and MS + 0.3 mg L-1 GA3 in the absence of light (30.5%. S. cineraria seeds were indifferent to light. Blue and green lights inhibited germination. Different concentrations of gibberellic acid (GA3 (0.1; 0.4; 0.6; 0.8; 1.0 and 2.0 mg L-1 and indole-3-acetic acid IAA (0.1; 0.3 and 1.0 mg L-1 were evaluated under white light and darkness. No concentration of GA3 enhanced seed germination percentage under white light. However, when the seeds were maintained in darkness, GA3 improved germination responses in all tested concentrations, except at 1.0 mg L-1. Under white light, these concentrations also increased the germination time and reduced germination rate. Germination rate, under light or darkness, was lower using IAA compared with GA3.

  2. A new eremophilane derivative from Senecio dianthus%双花千里光中一个新的艾里莫芬烷型倍半萜

    Institute of Scientific and Technical Information of China (English)

    韩贺东; 胡海清; 李琰; 王晓玲

    2013-01-01

    利用多种色谱技术对藏药双花千里光Senecio dianthus地上部分95%乙醇浸提物进行分离纯化,共得到5个化合物,根据理化数据和波谱数据鉴定化合物的结构分别为:艾里莫芬-9(10),7(11)-二烯-8-酮-12-羧酸-β-D-吡喃葡萄糖苷,命名为双花千里光苷A(1),5,7,4'-三羟基-黄酮-3-O-β-D-葡萄糖苷(2),槲皮素-3-O-β-D-葡萄糖苷(3),金丝桃苷(4),芦丁(5).其中,化合物1为新化合物,化合物2,4,5均为首次从双花千里光中分离得到.

  3. Microwave Extraction of Total Flavonoids from Senecio cannabifofius less and Optimization by Response Surface Method%返魂草总黄酮的微波提取及响应面优化

    Institute of Scientific and Technical Information of China (English)

    王志兵; 王力; 姜海峰; 沈海静

    2012-01-01

    探讨返魂草中总黄酮的提取方法和工艺条件。以返魂草总黄酮提取率为考察指标,比较了热回流提取法、超声波提取法和微波提取法的提取效果,并通过单因素试验和响应面分析法优化其工艺条件。试验结果表明:微波法提取返魂草中总黄酮的最优工艺条件为:料液比1∶52(g/mL),提取温度87℃,提取时间3.1 min,在此条件下总黄酮的提取率为4.827%。研究得到的工艺条件可用于返魂草总黄酮的提取。%To investigate extraction methods and technological conditions of total flavonoids in Senecio eannabifolius less, using extraction rates as main evaluating indicators, the extracting effects of thermore fluxing, ultrasonicwave and microwave extraction methods were compared in this experiment. At the same time, the extraction process was optimized by single factor experiment and response surface methodology. As a result, the optimum conditions for the extraction of flavonoids from senecio cannabifolius less obtained as follow: ratio of raw material to solvent was 1 : 52(g/mL), extraction temperature was 87 ℃, extraction time was 3.1 min. The average concentration of total flavonoids reached 4.827% under the optimum extracting conditions. The extraction process can be used for the extraction of total flavonoids in Senecio cannabifolius less.

  4. Mycophenolate mofetil as adjuvant in pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Sarma Nilendu

    2007-01-01

    Full Text Available Pemphigus vulgaris (PV is a life threatening autoimmune blistering disease of skin and mucous membranes. Advent of systemic steroids has greatly reduced the mortality rate. However, steroids and adjuvant immunosuppressive therapy are nowadays frequent contributory agents of morbidity and mortality of PV. Mycophenolate mofetil (MMF has been reported to be an effective adjuvant to systemic steroids. It helps in increasing the immunosuppressive effect and minimizing the toxicities by steroid sparing effect. However, its efficacy in refractory cases of PV is not well documented. The lowest possible dose with satisfactory therapeutic efficacy and least side effects is known. We used MMF 1 g/day and systemic steroids in 3 Indian patients with pemphigus vulgaris who were resistant to systemic steroid monotherapy or combination treatment with azathioprine. In our experience, MMF offers an effective adjuvant with minimal side-effects in the treatment of resistant PV.

  5. Light scattering on chlorella vulgaris cells

    Science.gov (United States)

    Krol, Tadeusz; Zielinski, Andrzej; Witkowski, Konrad

    1992-12-01

    Laboratory measurements of light scattering on the axenic cultures of unicellular alga Chlorella vulgaris monoculture confirm the thesis of multi-level light scattering by the cell i.e., both by outer cell membrane and the internal structure of the cell, as well as by its molecular structures. In the measurements, the technique of dynamic light scattering and analysis by the regulation method was used, indicate that the light scattering phenomenon is affected by particles of sizes corresponding either to overall dimensions of the cell or to the dimensions of its internal structures. A correlation was found between the suggested sizes and the stage of physiological evolution of the culture. The measurements of 10 functions constituting the elements of the scattering matrix for an alive Chlorella vulgaris culture and cultures with internal cell structures modified by chemical and mechanical agents evidence that the internal structures of cells play an important role in the interaction of phytoplankton and light.

  6. Giant lupus vulgaris: A rare presentation.

    Science.gov (United States)

    Sacchidanand, S; Sharavana, S; Mallikarjun, M; Nataraja, H V

    2012-01-01

    Cutaneous tuberculosis continues to be an important public health problem even with the availability of highly effective anti-tuberculous drugs. It constitutes 0.1% of all cases of extrapulmonary tuberculosis. Lupus vulgaris is the most common form of cutaneous tuberculosis that occurs in previously sensitized individuals with a moderate degree of immunity against tubercle bacilli. The different types of lupus vulgaris include plaque, ulcerative, vegetative, papular and nodular, and tumor forms. A 40-year-old man presented with large multiple plaques over right upper limb, right side of chest and back, and right lower limb for the past 30 years. Histopathology showed numerous noncaseating granulomas with Langhan's type of giant cells. The Mantoux test showed strong positivity and there was excellent response to anti-tuberculous treatment. This case is being reported because of its extreme chronicity of 30 years duration, unusually large size and multiplicity of lesions.

  7. Acne vulgaris related to androgens - a review.

    Science.gov (United States)

    Khondker, L; Khan, S I

    2014-01-01

    Sebum production is stimulated by androgens and is the key in the development of acne vulgaris. Several investigators have looked for direct relationships between serum androgen levels, sebum secretion rate and the presence of acne. The presence of acne in prepubertal girls and sebum production in both sexes correlate with serum dehydroepiandrosterone sulfate (DHEAS) levels. Although increased serum androgen levels correlate with the presence of severe nodular acne in men and women, these levels are often within the normal range in mild to moderate acne. This raises the question of whether there is an increased local production of androgens within the sebaceous gland of patients with acne vulgaris that leads to increased sebum secretion.

  8. Pemphigus vulgaris: a rare cause of dysphagia.

    Science.gov (United States)

    Al-Janabi, Ali; Greenfield, Simon

    2015-10-22

    Pemphigus vulgaris is a rare autoimmune blistering disease of the skin and mucous membranes. The case reported presented unusually with dyspepsia that was not responsive to protein pump inhibitor (PPI) therapy. This progressed to severe dysphagia and odynophagia. An esophagogastroduodenoscopy showed extensive ulceration of the esophagus, and direct immunofluorescence of an esophageal biopsy showed bright intercellular staining with C3 and IgG, confirming the diagnosis of pemphigus vulgaris. Immunological remission was achieved after a number of courses of pulsed intravenous methylprednisolone and cyclophosphamide. The patient has remained in remission for 5 years, but has required regular dilation of esophageal strictures for symptom relief. During this period, a chronic lymphocytosis was incidentally noted on routine blood tests, and chronic lymphocytic leukaemia was diagnosed. It is essential to investigate PPI-resistant symptoms, dysphagia and odynophagia, as they may indicate a serious underlying cause.

  9. The psychosocial impact of acne vulgaris

    Directory of Open Access Journals (Sweden)

    Neirita Hazarika

    2016-01-01

    Full Text Available Background: Acne vulgaris causes erythematous papulopustular lesions in active stage and often leave behind residual scarring and pigmentation. Its onset in adolescence may add to the emotional and psychological challenges experienced during this period. Aims: To assess the impact of acne on the various psychosocial domains of daily life. Materials and Methods: This was a prospective, cross-sectional study done in the dermatology out-patient department of a tertiary care hospital from January to March 2015. A total of 100 consecutive, newly diagnosed patients of acne vulgaris, aged 15 years and above were included in this study. The relationship between acne vulgaris and its sequelae was analyzed with ten different domains of daily life by using dermatology life quality index (DLQI questionnaire. Results: Females (56%, 15–20 year olds (61%, facial lesions (60%, and Grade II acne (70% were most common. Acne scars were noted in 75% patients, whereas 79% cases had post-acne hyperpigmentation. Thirty-seven percent patients had DLQI scores of (6–10 interpreted as moderate effect on patient's life. Statistically significant correlation (P < 0.05 found were as follows: Physical symptoms with grade of acne; embarrassment with site and grade of acne; daily activities with grade of acne and post-acne pigmentation; choice of clothes with site of acne; social activities with gender, site and grade of acne; effect on work/study with grade of acne; interpersonal problems with site and post-acne pigmentation; sexual difficulties with grade of acne. Limitation: It was a hospital-based study with small sample size. Conclusion: Significant impact of acne and its sequelae was noted on emotions, daily activities, social activities, study/work, and interpersonal relationships. Assurance and counseling along with early treatment of acne vulgaris is important to reduce disease-related psychosocial sequelae and increase the efficacy of treatment.

  10. The psychosocial impact of acne vulgaris

    OpenAIRE

    Neirita Hazarika; M Archana

    2016-01-01

    Background: Acne vulgaris causes erythematous papulopustular lesions in active stage and often leave behind residual scarring and pigmentation. Its onset in adolescence may add to the emotional and psychological challenges experienced during this period. Aims: To assess the impact of acne on the various psychosocial domains of daily life. Materials and Methods: This was a prospective, cross-sectional study done in the dermatology out-patient department of a tertiary care hospital from January...

  11. [ACNE VULGARIS--AETIOLOGY, CLASSIFICATION, TREATMENT].

    Science.gov (United States)

    Janda, Katarzyna; Chwilkowska, Magdalena

    2014-01-01

    A spotless skin is a rarity. Both women and men have different problems related to the complexion. One of the most common problems is acne, which affects an increasing number of people of all ages. Seborrhea skin areas rich in sebaceous glands, the formation of comedones, inflammation, and scars are characteristic for this disease. The aim of the study was to discuss the causes of acne vulgaris, methods of treatment, and proper care of the skin affected by this problem.

  12. The Historic Panorama of Acne Vulgaris

    OpenAIRE

    Humyra Tabasum; Tanzeel Ahmad; Farzana Anjum; Hina Rehman

    2013-01-01

    Although acne is described in very ancient writings dating back to Eber’s Papyrus, its clear description is found after Fuch’s coined the term ‘Acne Vulgaris’ and Erasmus Wilson separated it from acne rosacea. The early treatment of acne was based upon the witchcraft. Later new therapies got evolved with the discoveries in the field of anatomy, physiology and biochemistry. The following review focuses the historical overview of acne vulgaris, highlighting persons and discoveries in medival an...

  13. The Psychosocial Impact of Acne Vulgaris

    Science.gov (United States)

    Hazarika, Neirita; Archana, M

    2016-01-01

    Background: Acne vulgaris causes erythematous papulopustular lesions in active stage and often leave behind residual scarring and pigmentation. Its onset in adolescence may add to the emotional and psychological challenges experienced during this period. Aims: To assess the impact of acne on the various psychosocial domains of daily life. Materials and Methods: This was a prospective, cross-sectional study done in the dermatology out-patient department of a tertiary care hospital from January to March 2015. A total of 100 consecutive, newly diagnosed patients of acne vulgaris, aged 15 years and above were included in this study. The relationship between acne vulgaris and its sequelae was analyzed with ten different domains of daily life by using dermatology life quality index (DLQI) questionnaire. Results: Females (56%), 15–20 year olds (61%), facial lesions (60%), and Grade II acne (70%) were most common. Acne scars were noted in 75% patients, whereas 79% cases had post-acne hyperpigmentation. Thirty-seven percent patients had DLQI scores of (6–10) interpreted as moderate effect on patient's life. Statistically significant correlation (P embarrassment with site and grade of acne; daily activities with grade of acne and post-acne pigmentation; choice of clothes with site of acne; social activities with gender, site and grade of acne; effect on work/study with grade of acne; interpersonal problems with site and post-acne pigmentation; sexual difficulties with grade of acne. Limitation: It was a hospital-based study with small sample size. Conclusion: Significant impact of acne and its sequelae was noted on emotions, daily activities, social activities, study/work, and interpersonal relationships. Assurance and counseling along with early treatment of acne vulgaris is important to reduce disease-related psychosocial sequelae and increase the efficacy of treatment. PMID:27688440

  14. Insulin resistance in severe acne vulgaris.

    Science.gov (United States)

    Emiroğlu, Nazan; Cengiz, Fatma Pelin; Kemeriz, Funda

    2015-08-01

    Acne vulgaris is a pilosebaceous gland disease that usually affects people from puberty to young adulthood. It is seen especially on the face, neck, trunk and arms. Its severity differs from patient to patient and its pathogenesis is multifactorial. The main pathogenic factors of acne are high sebaceous gland secretion, follicular hyperproliferation, high androgen effects, propionibacterium acnes colonization and inflammation. Diet is always thought a probable reason for acne and many studies are done about acne and diet. To determine the effect of insulin resistance in severe acne vulgaris. Two hundred and forty-three acne vulgaris patients and 156 healthy controls were enrolled into the study. The blood levels of insulin and glucose were measured. Homeostasis Model Assessment (HOMA) Index was calculated. The values were compared with the control group. All of the patients were in the severe acne group according to their scores on the global acne scoring scale. While fasting blood glucose levels were not different between the groups (p > 0.05, 82.91 ±9.76 vs. 80.26 ±8.33), the fasting insulin levels were significantly higher in the patient group than in the control group (p acne.

  15. Pemfigus Vulgaris Manifestasi di Mulut dan Penatalaksanaannya

    Directory of Open Access Journals (Sweden)

    Metawati Tarmidi

    2015-11-01

    Full Text Available Pemfigus is an autoimmune disease involving the skin and mucosa and characterized by intraepidermal bullae formation. The four variations of pemphigus are pemphigus vulgaris, vegetans, foliaceus, and erythematosus. Although several clinical variants are recognized, pemphigus vulgaris is the most common form and the type most likely to exhibit oral manifestations. There are from 0,1 to 0,5 cases reported each year per 100,000 population with the highest incidence occurring in the fourth and seventh decades of life. Only rare cases have been reported in children and the elderly. Before the advent of systemic corticosteroid therapy, pemphigus vulgaris was fatal within few months to two years. A characteristic feature is the nikolsky sign which is the ability to elicit the formation of bullae by the application of firm lateral pressure on normal-appearing skin. The oral lesions often have a slow, insidious onset with symptoms present for many months before a diagnosis is made or skin lesions develop. Lesions may be found anywhere but are most common on the palate, buccal mucosa, and gingiva. Although the oral lesions are vesicobullous in nature, intact blisters are rarely seen. Diagnosis is established by biopsy and immunofluorescent studies. Titers on indirect immunofluorescence often correlate to the severity of the disease. The aim of this study is to promote and support early dental diagnosis when the prognosis is good.

  16. 千里光花黄色素提取及稳定性研究%Study on extraction and stability of yellow pigment from Senecio

    Institute of Scientific and Technical Information of China (English)

    肖浩; 郑小江

    2011-01-01

    The extraction conditions and stability of Senecio pigment were studied using Senecio flowers yellow pigment characteristic absorbance value as index. Results showed that extraction rate was higher after extraction 3h at 60℃ with ethyl acetate in the solid-liquid ratio as 1 :25,the pigment was easy to change under acid conditions but stable under alkaline conditions. High temperature had little effect on the pigment stability, strong sunlight can speed up the pigment degradate, natural light in room had little effect. Strong oxidant had little effect on the pigment stability,reductant had a certain impact on the stability of the pigment, additives as sodium benzoate,sucrose,soluble starch and salt which used to add in food had no effect on the pigment. Such metallic ions as Cu2+ , Al3+ , Fe3+ had significant effects on stability of the pigment,while metallic ions as Na+, K +, Ca2+ ,Zn2+ had no effects. It was functional plant pigment which can be widely used in food, pharmaceutical and cosmetics industries.%采用有机溶剂浸提法,以千里光花黄色素特征吸光度值为指标,探讨了千里光花黄色素的提取条件和稳定性.结果表明:用1:25的乙酸乙酯在60℃恒温浸提3h为最佳提取工艺条件;该色素为脂溶性色素,在强酸中极不稳定,而在碱性条件下稳定;高温对该色素的稳定性影响不明显,强日光和紫外光会加快色素降解,室内自然光对该色素的稳定性影响不明显;强氧化剂对该色素的稳定性影响不明显,还原剂对该色素的稳定性有一定影响;苯甲酸钠、蔗糖、可溶性淀粉和盐等常用食品添加剂对该色素的稳定性无影响;金属离子Cu2+、Al3+、Fe3+对该色素的稳定性影响显著,Na+、K+、Ca2+、Zn2+等离子对该色素的稳定性无影响;该色素为功能性植物色素,可广泛应用于食品、医药和化妆品等行业.

  17. Extensive range persistence in peripheral and interior refugia characterizes Pleistocene range dynamics in a widespread Alpine plant species (Senecio carniolicus, Asteraceae).

    Science.gov (United States)

    Escobar García, Pedro; Winkler, Manuela; Flatscher, Ruth; Sonnleitner, Michaela; Krejčíková, Jana; Suda, Jan; Hülber, Karl; Schneeweiss, Gerald M; Schönswetter, Peter

    2012-03-01

    Recent evidence suggests that survival of arctic-alpine organisms in peripheral or interior glacial refugia are not mutually exclusive and may both be involved in shaping an organism's Pleistocene history, yet potentially at different time levels. Here, we test this hypothesis in a high-mountain plant (diploid lineage of Senecio carniolicus, Asteraceae) from the Eastern European Alps, in which patterns of morphological variation and current habitat requirements suggest survival in both types of refugia. To this end, we used AFLPs, nuclear and plastid DNA sequences and analysed them, among others, within a graph theoretic framework and using novel Bayesian methods of phylogeographic inference. On the basis of patterns of genetic diversity, occurrence of rare markers, distribution of distinct genetic lineages and patterns of range connectivity both interior refugia in the formerly strongly glaciated central Alps and peripheral refugia along the southern margin of the Alps were identified. The presence of refugia congruently inferred by markers resolving at different time levels suggests that these refugia acted as such throughout several glacial cycles. The high degree of range persistence together with gradual range expansion, which contrasts with the extent of range shifts implied for other Alpine species, is likely responsible for incipient lineage differentiation evident from the genetic data. Replacing a simplistic peripheral vs. interior refugia dualism by more complex models involving both types of refugia and considering different time levels will help identifying common phylogeographic patterns with respect to, for instance, location of refugia and colonization routes and elucidating their underlying genetic and/or ecological causes. DNA sequences have been deposited in GenBank under accession nos. FR796701–FR797793 and nos. HE614296–HE614583.

  18. Extracción, caracterización y evaluación de la actividad antibacteriana del aceite esencial de Senecio graveolens Wedd (Wiskataya

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    Kiev Ochoa Pumaylle

    2012-01-01

    Full Text Available El objetivo del presente trabajo fue extraer, caracterizar y evaluar la actividad antibacteriana del aceite esencial de Senecio graveolens Wedd (Wiskataya frente a Escherichia coli y Staphylococcus aureus ATCC 29923. Las hojas y tallos se recolectaron a una altitud de 3800 m.s.n.m. en el distrito de Puquio, provincia de Lucanas, departamento de Ayacucho. El aceite esencial se obtuvo por destilación por arrastre con vapor de agua, a partir de las hojas y tallos desecados de S. graveolens, con rendimiento de 1,26 % (p/p. La muestra extraída fue caracterizada a través de ensayos físicos. La composición química del aceite se evaluó mediante cromatografía de gas con detector de masa (CG-SM. La actividad antibacteriana del aceite de S. graveolens se realizó por el método de difusión en agar en pocillos, utilizando cepas de microorganismos gram positivo como S. aureus y gram negativo como E. coli. La densidad del producto resultó 0,8755 g/ml a 20 ºC; índice de refracción 1,4726; índice de rotación 102°85’ y soluble en etanol; el cromatograma mostró componentes mayoritarios con un contenido de 52,39 % Sabineno, 8,20 % (+-4-careno, 7,11 % τ-terpineno, 6,74 % β-myrceno, 3,78 % 4-terpinenol, 3,67 % Pulegona. Los resultados mostraron actividad antibacteriana marcada y moderada, para S. aureus y E. coli, respectivamente, observándose formación de halos de inhibición para concentraciones del aceite esencial a 80, 90 y 100 %. El aceite esencial de S. graveolens se presenta con actividad antibacteriana promisoria.

  19. Prenatal exposure to integerrimine N-oxide enriched butanolic residue from Senecio brasiliensis affects behavior and striatal neurotransmitter levels of rats in adulthood.

    Science.gov (United States)

    Sandini, Thaísa M; Udo, Mariana S B; Reis-Silva, Thiago M; Sanches, Daniel; Bernardi, Maria Martha; Flório, Jorge Camilo; Spinosa, Helenice de S

    2015-12-01

    Pyrrolizidine alkaloids (PAs) are toxins that are exclusively biosynthesized by plants and are commonly present in foods and herbs. PAs are usually associated with poisoning events in livestock and human beings. The aim of the present study was to evaluate the behavioral and neurochemical effects of prenatal exposure to PA integerrimine N-oxide of rats in adulthood. Pregnant Wistar rats received integerrimine N-oxide from the butanolic residue of Senecio brasiliensis by gavage on gestational days 6-20 at doses of 3, 6 and 9 mg/kg. During adulthood of the offspring, the following behavioral tests were performed: open-field, plus-maze, forced swimming, catalepsy and stereotypy. Histological analyses and monoamine levels were measured. Male offspring from dams that were exposed to 9 mg/kg showed an increase in locomotion in the open-field test, an increased frequency of entries and time spent in open arms in elevated plus-maze test, as well as decreased swimming time. In the female offspring from dams that were exposed to 9 mg/kg, there was an increased time of climbing in forced swimming and intensity of stereotyped behavior. The histological study indicates an increase in the number of multinucleated cells in the liver (6 and 9 mg/kg). In neurotransmitter analysis, specifically in the striatum, we observed change in dopamine and serotonin levels in the middle dose. Thus, our results indicate that prenatal exposure to integerrimine N-oxide changed behavior in adulthood and neurotransmitter levels in the striatum. Our results agree with previous studies, which showed that integerrimine N-oxide impaired physical and neurobehavioral development in childhood that can persist until adulthood. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Patrones electroforéticos de proteínas y actividad anticongelante en el apoplasto de la hoja de la especie andina tropical Senecio niveoaureus PROTEIN ELECTROPHORETIC PATTERNS AND ANTIFREEZING ACTIVITY IN THE LEAF APOPLAST OF THE TROPICAL ANDEAN SPECIES Senecio niveoaureus

    Directory of Open Access Journals (Sweden)

    F ÁLVAREZFLÓREZ

    2006-06-01

    Full Text Available Las plantas de alta montaña tienen diferentes adaptaciones para sobrevivir a cambios drásticos de temperatura, especialmente a condiciones de congelamiento. En plantas de invierno, la supervivencia a temperaturas bajas está relacionada con la capacidad de las células para producir proteínas específicas de bajo peso molecular (proteínas anticongelantes y exportarlas al apoplasto. Para establecer si plantas tropicales de alta montaña sobreviven las temperaturas bajas a través del mismo mecanismo, se colectaron hojas de plantas de Senecio niveoaureus durante 24 horas y a dos alturas 3.300 y 3.600 msnm en el Páramo de Palacio, Chingaza, Colombia. Se observaron proteínas apoplásticas de pesos moleculares entre 3512 kDa. Los patrones electroforéticos fueron diferentes dependiendo de la altura y la hora de muestreo, sin embargo, se observaron variaciones en el patrón de bandeo que no pueden ser atribuidas ni a la temperatura ni al gradiente altitudinal únicamente. Se detectó actividad anticongelante en el apoplasto de hojas de S. niveoaureus, siendo este el primer reporte en especies tropicales de alta montaña.Tropical high mountain plants have different adaptations to survive extreme daily temperature fluctuations and specially freezing night conditions. In winter plant species, survival to low temperatures is related to the ability of the cell to produce specific low molecular weight proteins (antifreezing proteins and to export them to the apoplast. In order to see if high mountain tropical plants survive to low temperatures through the same mechanism we collected, during a 24 hourperiod, leaves from Senecio niveoaureus growing at 3,300 and 3,600 m.o.s.l, in the Páramo de Palacio, Chingaza, Colombia. Leaf apoplast proteins had MW between 3512 kDa. Electrophoretic patterns were different depending on the altitude and the time of sampling. However the observed variations could not be linked to changes in temperature or to the

  1. Pyrrolizidine alkaloids of Senecio cannabifolius var.integrilifolius%单麻叶千里光中肝毒吡咯里西啶生物碱的分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    马鸿雁; 杨莉; 王长虹; 王峥涛

    2011-01-01

    Objective: To study the pyrrolizidine alkaloids of Senecio cannabifolius var. integrilifolius. Method: Variouschromatographic methods were used for the isolation and purification of chemical constitutents of the plant and their structures were elucidated on the basis of spectral analysis. Result: Four compounds were determinated as jacobine( 1 ) ,jacoline(2 ), jaconine(3), senecicannabine(4). Conclusion: All compounds were isolated from this plant for the first time.%目的:研究菊科千里光属植物单麻叶千里光Senecio cannabifolius var.integrilifolius的肝毒性吡咯里西啶生物碱.方法:运用多种色谱方法进行分离纯化,应用现代波谱学分析方法进行结构鉴定.结果:从单麻叶千里光的总碱部位中分离得到4个吡咯里西啶生物碱,分别鉴定为:jacobine(1),jacoline(2),jaconine(3),senecicannabine(4).结论:化合物1~4均为首次从该植物中分离得到.

  2. Variation in the Breeding System of Prunella vulgaris L.

    Science.gov (United States)

    Prunella vulgaris (Lamiaceae), commonly known as selfheal, is a perennial herb with a long history of use in traditional medicine. Recent studies have found that P. vulgaris possesses anti-inflammatory, antiviral and anti-bacterial properties, which may lead to increased commercial demand. To date...

  3. A new pyrroloquinazoline alkaloid from Linaria vulgaris.

    Science.gov (United States)

    Hua, Huiming; Cheng, Maosheng; Li, Xian; Pei, Yuehu

    2002-10-01

    A new alkaloid, 1,2,3,9-tetrahydropyrrolo(2,1-b)quinazolin-1-carboxylic acid (1), together with eight known compounds, 7-hydroxy vasicine (2), benzyl alcohol beta-D-(2'-O-beta-xylopyranosyl)glucopyranoside (3), benzyl alcohol O-beta-D-glucopyranoside (4), benzyl alcohol O-beta-D-primveroside (5), 3,5-dimethyl-4-hydroxy benzaldehyde (6), gluco-syringic acid (7), syringin (8), and liriodendrin (9), were isolated from the plants of Linaria vulgaris. Their structures were established by spectroscopic methods.

  4. The Historic Panorama of Acne Vulgaris

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    Humyra Tabasum

    2013-09-01

    Full Text Available Although acne is described in very ancient writings dating back to Eber’s Papyrus, its clear description is found after Fuch’s coined the term ‘Acne Vulgaris’ and Erasmus Wilson separated it from acne rosacea. The early treatment of acne was based upon the witchcraft. Later new therapies got evolved with the discoveries in the field of anatomy, physiology and biochemistry. The following review focuses the historical overview of acne vulgaris, highlighting persons and discoveries in medival and modern period.

  5. Annular lupus vulgaris mimicking tinea cruris.

    Science.gov (United States)

    Heo, Young Soo; Shin, Won Woong; Kim, Yong Ju; Song, Hae Jun; Oh, Chil Hwan

    2010-05-01

    Cutaneous tuberculosis is an infrequent form of extrapulmonary tuberculosis. It is often clinically and histopathologically confused with various cutaneous disorders. A 36-year-old man attended our clinic with slowly progressive, asymptomatic, annular skin lesions on both the thighs and buttocks for 10 years. He consulted with many physicians and was improperly treated with an oral antifungal agent for several months under the diagnosis of tinea cruris, but no resolution of his condition was observed. A diagnosis of lupus vulgaris was made based on the histopathologic examination and the polymerase chain reaction assay. Anti-tuberculosis therapy was administered and the lesions started to regress.

  6. Clinico- Pathological Study Of Ichthyosis Vulgaris

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    Nandy Utpal

    1997-01-01

    Full Text Available A clinico- pathological study of 28 cases of ichthyosis vulgaris appeared with in the age of 5 years. The presence of the disease since birth was also found. While most (24 patients showed a diminution of severity in summer with an aggravation during winter, 4 patients followed the opposite seasonal pattern. Only in 4 patients, fine scales in the scalp were detected. One patient showed an affection of flexures. There was also a low occurrence of palmo- planter hyperkeratosis, follicular keratosis, fissuring of hands and feet and atopy.

  7. Disseminated lupus vulgaris: a case report.

    Science.gov (United States)

    Can, Burce; Zindanci, Ilkin; Turkoglu, Zafer; Kavala, Mukaddes; Ulucay, Vasfiye; Demir, Filiz Topaloglu

    2014-01-01

    Lupus vulgaris is a secondary form of cutaneous tuberculosis which persists for years if not treated. The head and neck are the most commonly affected sites. While less frequently arms and legs, and rarely the trunk and the scalp are involved. Herein, we describe a 73-year-old man with a 5-year history of slowly growing, atrophic, some eroded and ulcerated, red-brown plaques on his forehead, nose, cheeks, ear lobes, trunk and extremites. All of his disseminated lesions healed after antituberculosis therapy.

  8. Lupus vulgaris in a young girl.

    Science.gov (United States)

    Goyal, Tarang; Varshney, Anupam; Bakshi, S K

    2013-01-01

    With the estimated global burden of TB being 8.8 million incident cases and 1.1 million deaths from TB in HIV-negative cases and additional 0.35 million deaths in HIV-associated cases,1 the total number of cutaneous TB cases ( lupus vulgaris in a young girl with rapid progression of a large plaque with hypertrophic features in the periphery. The case is unusual due to its rapid progression, unusual site and extensive giant form which have never been reported previously.

  9. Annular Lupus Vulgaris Mimicking Tinea Cruris

    Science.gov (United States)

    Heo, Young Soo; Shin, Won Woong; Kim, Yong Ju; Song, Hae Jun

    2010-01-01

    Cutaneous tuberculosis is an infrequent form of extrapulmonary tuberculosis. It is often clinically and histopathologically confused with various cutaneous disorders. A 36-year-old man attended our clinic with slowly progressive, asymptomatic, annular skin lesions on both the thighs and buttocks for 10 years. He consulted with many physicians and was improperly treated with an oral antifungal agent for several months under the diagnosis of tinea cruris, but no resolution of his condition was observed. A diagnosis of lupus vulgaris was made based on the histopathologic examination and the polymerase chain reaction assay. Anti-tuberculosis therapy was administered and the lesions started to regress. PMID:20548922

  10. Nootropic effect of meadowsweet (Filipendula vulgaris) extracts.

    Science.gov (United States)

    Shilova, I V; Suslov, N I

    2015-03-01

    The effects of the extracts of the aboveground parts of Filipendula vulgaris Moench on the behavior and memory of mice after hypoxic injury and their physical performance in the open-field test were studied using the models of hypoxia in a sealed volume, conditioned passive avoidance response (CPAR), and forced swimming with a load. The extracts improved animal resistance to hypoxia, normalized orientation and exploration activities, promoted CPAR retention after hypoxic injury, and increased physical performance. Aqueous extract of meadowsweet had the most pronounced effect that corresponded to the effect of the reference drug piracetam. These effects were probably caused by modulation of hippocampal activity.

  11. The association of acne vulgaris with diet.

    Science.gov (United States)

    Veith, Whitney B; Silverberg, Nanette B

    2011-08-01

    Acne vulgaris is a common disorder of the pilosebaceous unit affecting adolescents and adults. The rise in incidence of acne in the United States may relate to the Western diet--which is rich in dairy and refined sugars, and high in glycemic load--and to the obesity epidemic. We reviewed the literature on diet and acne. Evidence exists highlighting the association of acne and high glycemic loads, certain dairy products, and refined sugar product ingestion. Future studies are required to determine if dietary modification will reduce long-term acne burden.

  12. Hormonal treatment of acne vulgaris: an update

    Science.gov (United States)

    Elsaie, Mohamed L

    2016-01-01

    Acne vulgaris is a common skin condition associated with multiple factors. Although mostly presenting alone, it can likewise present with features of hyperandrogenism and hormonal discrepancies. Of note, hormonal therapies are indicated in severe, resistant-to-treatment cases and in those with monthly flare-ups and when standard therapeutic options are inappropriate. This article serves as an update to hormonal pathogenesis of acne, discusses the basics of endocrinal evaluation for patients with suspected hormonal acne, and provides an overview of the current hormonal treatment options in women. PMID:27621661

  13. Pemphigus vulgaris and amyotrophic lateral sclerosis

    Science.gov (United States)

    Mokhtari, Fatemeh; Matin, Marzieh; Rajati, Fatemeh

    2016-01-01

    Pemphigus vulgaris (PV) is an autoimmune bullous and erosive mucocutaneous disease. Rarely, it occurs in patients with other autoimmune disease. The relation between PV and neurological disorders is unclear and needs to be more studied. Here, we report a case of amyotrophic lateral sclerosis (ALS), followed by dermatologic involvement. Histopathological evidence and direct immunofluorescence are consistent with PV. Systemic corticosteroid and azathioprine were effective in the treatment of mucocutaneous lesions. PV seems to be accidentally associated with ALS. Expression of major histocompatibility complex Class II in autoimmune disease and production of autoantibodies have been proposed to describe the association of PV with ALS. PMID:28163728

  14. Simultaneous quantitation of adonifoline and senecionine in Senecio herbs by UPLC-MS%UPLC-MS同时测定千里光和欧洲千里光中阿多尼弗林碱和千里光碱的含量

    Institute of Scientific and Technical Information of China (English)

    熊爱珍; 杨莉; 杨雪晶; 王长虹; 王峥涛

    2011-01-01

    Objective: To develop an UPLC-MS method for the simultaneously quantitation of adonifoline and senecinine in Senecio herbs.Method: UPLC-Micro 2000 was used for quantification in SIR mode under ESI+.Monocrotaline was used as the internal standard.Chromatography was performed on a Shiseido Capcell Pak MG (2.0 mm × 50 mm, 3 μm) column at 30 ℃ using an gradient elution of acetonitrile-0.5% formic acid in water at the flow rate of 0.3 mL · min-1.The injection volume was 2 μL.Result: Good linearity was obtained for quantitation of adonifoline over the range of 1.02 -816.00 μg · L-1 ( r =0.998 0).And recoveries at different concentration levels were between 95.73% and 103.0% with RSDs no more than 2.5%.For quantification of senecionine, the linear range was between 1.08-860.56 μg · L-1 ( r =0.997 6).And recoveries at different concentration levels were between 95.67% and 101.5% with RSDs no more than 2.3%.Good reproducibility and precision were also achieved.Conclusion: The new developed UPLC method is sensitive, accurate and reliable enough for the quantitation of adonifoline and senecionine in Senecio herbs thus can be used for the limit detection of pyrrolizidne alkaloids in S.scandens.It can also be used for the identification of fake drugs of S.scandens such as S.vulgaris.The developed method was served for the quality evaluation of Herba Senecionis Scandentis.%目的:建立同时测定千里光和欧洲千里光中阿多尼弗林碱和千里光碱含量的UPLC-MS方法.方法:采用Wa-ters公司UPLC-Micro 2000仪器ESI+离子模式下选择离子监控,以野百合碱为内标计算浓度;色谱分离采用Shiseido Capcell Pak MG色谱柱(2.0 mm × 50 mm,3 μm),流动相乙腈-0.5%甲酸水溶液梯度洗脱,流速0.3 mL·min-1,柱温30℃,进样量2μL.结果:阿多尼弗林碱进样质量浓度在1.02~816.00 μg· L-1呈现良好线性关系(r=0.998 0),50%,100%,150%浓度水平下加样回收率在95.73%~103.0%,RSD不超过2.5%;千

  15. Berberis vulgaris for cardiovascular disorders: a scoping literature review

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    Abdelrahman Abushouk

    2017-05-01

    Full Text Available Cardiovascular disorders are the leading cause of mortality worldwide. Berberis vulgaris (B. vulgaris is a commonly used plant in traditional medicine. In recent studies, B. vulgaris showed antiarrhythmic, antihypertensive, anticholinergic, and cardioprotective effects. We reviewed the literature to explore the possible prophylactic and therapeutic roles of B. vulgaris in cardiovascular medicine. A computer literature search was conducted to identify all relevant studies that have investigated the role of B. vulgaris in prevention or treatment of cardiovascular diseases.We also searched the citations of the retrieved articles. Using a systematic approach, we conducted a scoping review that included a total of 37 articles. Twelve studies examined the antihypertensive effects of B. vulgaris, seven studies investigated its antiarrhythmic effects, while its inotropic and cardioprotective effects were evaluated in four and eight studies, respectively. B. vulgaris showed a beneficial effect in reducing blood pressure, enhancing cardiac contractility, and protection from reperfusion injury. However, the mechanisms of these effects are still under investigation. Moreover, it could modify major risk factors for cardiovascular disorders, such as oxidative stress, hyperglycemia, and hyperlipidemia. Further studies are needed to translate these findings into effective cardiovascular medications.

  16. The epidemiology of acne vulgaris in late adolescence.

    Science.gov (United States)

    Lynn, Darren D; Umari, Tamara; Dunnick, Cory A; Dellavalle, Robert P

    2016-01-01

    Acne vulgaris is the most common skin condition affecting late adolescents across the globe. Although prior studies have evaluated epidemiologic patterns of acne vulgaris in various ethnicities and regions, adequate understanding of the worldwide burden of the disease associated with patients in their late adolescence (15-19-year olds) remains lacking. To assess the global burden of the disease associated with acne vulgaris for late adolescents (15-19-year olds) and provide an overview of the epidemiology, pathophysiology, and treatment options for acne in this population. Database summary study. Global Burden of Disease Study 2010 database. Global Burden of Disease regions comprised countries with prevalence of acne vulgaris between the ages of 15 and 19 years. Geographic region-level disability-adjusted life year rates (per 100,000 persons) associated with acne vulgaris in years 1990 through 2010. Median percentage change in disability-adjusted life year rates was estimated for each region across the specified study period. Acne vulgaris-associated disease burden exhibits global distribution and has continued to grow in prevalence over time within this population. This continued growth suggests an unmet dermatologic need worldwide for this disorder and potential opportunities for improved access and delivery of dermatologic care. Our analysis of the literature reveals numerous opportunities for enhanced patient care. To that end, we highlight some of the effective and promising treatments currently available and address important factors, such as sex, nationality, genetics, pathophysiology, and diet, as they relate to acne vulgaris in late adolescence.

  17. Role of olfaction in Octopus vulgaris reproduction.

    Science.gov (United States)

    Polese, Gianluca; Bertapelle, Carla; Di Cosmo, Anna

    2015-01-01

    The olfactory system in any animal is the primary sensory system that responds to chemical stimuli emanating from a distant source. In aquatic animals "Odours" are molecules in solution that guide them to locate food, partners, nesting sites, and dangers to avoid. Fish, crustaceans and aquatic molluscs possess sensory systems that have anatomical similarities to the olfactory systems of land-based animals. Molluscs are a large group of aquatic and terrestrial animals that rely heavily on chemical communication with a generally dispersed sense of touch and chemical sensitivity. Cephalopods, the smallest class among extant marine molluscs, are predators with high visual capability and well developed vestibular, auditory, and tactile systems. Nevertheless they possess a well developed olfactory organ, but to date almost nothing is known about the mechanisms, functions and modulation of this chemosensory structure in octopods. Cephalopod brains are the largest of all invertebrate brains and across molluscs show the highest degree of centralization. The reproductive behaviour of Octopus vulgaris is under the control of a complex set of signal molecules such as neuropeptides, neurotransmitters and sex steroids that guide the behaviour from the level of individuals in evaluating mates, to stimulating or deterring copulation, to sperm-egg chemical signalling that promotes fertilization. These signals are intercepted by the olfactory organs and integrated in the olfactory lobes in the central nervous system. In this context we propose a model in which the olfactory organ and the olfactory lobe of O. vulgaris could represent the on-off switch between food intake and reproduction.

  18. Lupus vulgaris: unusual presentation on face.

    Science.gov (United States)

    Pilani, A; Vora, R V

    2014-01-01

    Lupus vulgaris is a variant of cutaneous tuberculosis. As the disease has potential to mutilate when left untreated, leaving deforming scars and disfigurement, an early diagnosis is of paramount importance. Though the common type is plaque type, rarely mutilating and vegetative forms also are found. A 28 year old female, labourer presented with progressive annular plaque over right side of cheek extending upto right lower lid and ala of nose. There were two satellite plaques near the right side of giant lesion. On diascopy apple jelly nodule was seen. There was no regional lymhadenopathy. Histopathological examination showed many granulomas in upper dermis extending to deep dermis comprising of epitheloid cells with langhans' type of giant cells, lymphocytic infiltration & focal necrosis suggestive of lupus vulgaris. The consequences of failing to make an early diagnosis can be disastrous for the patients, as the progression of the disease can lead to necrosis, destruction of bones and cartilage leading to permanent deformity. Thus it is vital for clinicians to have a high index of suspicion of such atypical forms and take biopsy samples for histological and bacteriological studies.

  19. Calcinosis cutis secondary to facial acne vulgaris: A rare complication

    Directory of Open Access Journals (Sweden)

    Srimanta Kumar Sahu

    2015-01-01

    Full Text Available Acne vulgaris is a common dermatological disease commonly affecting the adolescent and young adults. It is characterized by the presence of pleomorphic skin lesions such as comadones, papules, pustules, and nodules. The common complications are postacne hyperpigmentation and scarring causing psychological impact. Calcinosis cutis is the pathologic deposition of insoluble calcium salt in the skin and subcutaneous tissue. Calcinosis cutis following acne vulgaris is rarely reported in the literature. We report a case of calcinosis cutis in acne vulgaris in a 55-year-old man.

  20. Calcinosis cutis secondary to facial acne vulgaris: A rare complication.

    Science.gov (United States)

    Sahu, Srimanta Kumar; Gupta, Nikhil; Vohra, Suruchi

    2015-12-01

    Acne vulgaris is a common dermatological disease commonly affecting the adolescent and young adults. It is characterized by the presence of pleomorphic skin lesions such as comadones, papules, pustules, and nodules. The common complications are postacne hyperpigmentation and scarring causing psychological impact. Calcinosis cutis is the pathologic deposition of insoluble calcium salt in the skin and subcutaneous tissue. Calcinosis cutis following acne vulgaris is rarely reported in the literature. We report a case of calcinosis cutis in acne vulgaris in a 55-year-old man.

  1. Vascular cognitive impairment in Pemphigus vulgaris: a case report

    Directory of Open Access Journals (Sweden)

    José Ibiapina Siqueira- Neto

    Full Text Available ABSTRACT Pemphigus vulgaris is a systemic auto-immune medical condition that mainly manifests with changes in skin and vasculopathy. This is a case report of a 69-year-old male with confirmed histopathologic diagnosis of Pemphigus vulgaris presenting ulterior Cognitive Impairment, mostly in executive function. The patient was treated using steroids, immunomodulatory therapy, fluoxetine and galantamine. Neuropsychological testing and magnetic resonance (MRI were performed. This is the first report of correlational cognitive impairment with Pemphigus vulgaris in the literature. Physicians should be aware of vascular causes for cognitive impairment in patients presenting auto-immune conditions.

  2. Intoxicação experimental aguda por Senecio brasiliensis em ovinos e indução de resistência à intoxicação

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    Fabiane B. Grecco

    2012-09-01

    Full Text Available Ovinos são mais resistentes à intoxicação por Senecio spp. que bovinos e equinos. Para determinar se essa resistência é induzida pela ingestão de pequenas e repetidas doses da planta e se essa resistência é duradoura, foram realizados três experimentos com folhas e talos verdes de Senecio brasilienses. Para determinar a dose mínima que causa intoxicação aguda (experimento 1, foram administradas doses únicas de 60, 80, 90, 100 e 100g/kg de peso corporal (pc a cinco ovinos, respectivamente. Os animais que receberam 60 e 80 g/kg de pc de S. brasiliensis não adoeceram, porém o ovino que recebeu 80g/kg de pc apresentava fibrose e megalocitose discretas nas biópsias realizadas aos 90, 120 e 150 dias do término da administração da planta. Os ovinos que receberam 90 e 100g/kg de pc apresentaram anorexia, prostração, movimentos de pedalagem, dor abdominal e morte 12-48 horas após o aparecimento dos sinais clínicos. Na necropsia havia ascite, petéquias disseminadas e acentuação do padrão lobular hepático. Histologicamente havia necrose hemorrágica centro-lobular. No Experimento 2 a dose capaz de causar a intoxicação aguda foi fracionada e administrada em duas, cinco e 10 doses diárias para 3 ovinos, respectivamente. A dose tóxica fracionada não provocou sinais clínicos de intoxicação em nenhum dos ovinos, porém havia fibrose periportal e megalocitose moderadas nas biopsias realizadas aos 60 dias do término da administração da planta, as quais não evoluíram. O ovino que recebeu a dose fracionada em 10 administrações não apresentou lesões histológicas nas biópsias. Para determinar se os ovinos tornam-se resistentes à forma aguda da intoxicação (experimento 3, foram administradas doses diárias de 15g/kg de pc por 30 dias e 30g/kg de pc por 10 dias a quatro ovinos. No dia seguinte à última administração dois ovinos receberam a dose única de 100g/kg de pc de S. brasiliensis, mas não adoeceram nem

  3. Synthesis of (3,5-/sup 14/C)trachelanthamidine and (5-/sup 3/H)isoretronecanol and their incorporation into the retronecine moiety of riddelliine in Senecio riddellii

    Energy Technology Data Exchange (ETDEWEB)

    Leete, E.; Rana, J.

    1986-09-01

    (+/-)-(3,5-/sup 14/C)Trachelanthamidine and (+/-)-(5-/sup 3/H)isoretronecanol, which are diastereomers, were prepared from potassium (/sup 14/C)cyanide and (5-/sup 3/H)proline, respectively. These compounds and (1,4-/sup 14/C)putrescine were administered to Senecio riddellii plants resulting in the formation of labeled riddelliine, in which almost all the radioactivity was located in its retronecine moiety. The activity of the beta-alanine obtained by degradation of the retronecine was consistent with specific labeling of this pyrrolizidine base at the expected positions. The extremely high absolute incorporation (15.1, 22.1%) of trachelanthamidine into riddelliine strongly favors this 1-hydroxymethylpyrrolizidine as the one on the main biosynthetic pathway to retronecine. The lower incorporation (0.75%) of isoretronecanol may represent a minor or aberrant pathway to retronecine.

  4. Propionibacterium acnes in the pathogenesis and immunotherapy of acne vulgaris.

    Science.gov (United States)

    Liu, Pei-Feng; Hsieh, Yao-Dung; Lin, Ya-Ching; Two, Aimee; Shu, Chih-Wen; Huang, Chun-Ming

    2015-01-01

    Acne vulgaris, a multi-factorial disease, is one of the most common skin diseases, affecting an estimated 80% of Americans at some point during their lives. The gram-positive and anaerobic Propionibacterium acnes (P. acnes) bacterium has been implicated in acne inflammation and pathogenesis. Therapies for acne vulgaris using antibiotics generally lack bacterial specificity, promote the generation of antibiotic-resistant bacterial strains, and cause adverse effects. Immunotherapy against P. acnes or its antigens (sialidase and CAMP factor) has been demonstrated to be effective in mice, attenuating P. acnes-induced inflammation; thus, this method may be applied to develop a potential vaccine targeting P. acnes for acne vulgaris treatment. This review summarizes reports describing the role of P. acnes in the pathogenesis of acne and various immunotherapy-based approaches targeting P. acnes, suggesting the potential effectiveness of immunotherapy for acne vulgaris as well as P. acnes-associated diseases.

  5. Plant Anticancer Agents XXXIII. Constituents of Passerina vulgaris1.

    Science.gov (United States)

    Ji-Xian, G; Handa, S S; Pezzuto, J M; Kinghorn, A D; Farnsworth, N R

    1984-06-01

    Two lignans of known structure, (+)-syringaresinol, a cytotoxic agent, and (+)-nortrachelogenin, a compound with demonstrated antileukemic activity, were isolated from a biologically active extract of the stems of PASSERINA VULGARIS.

  6. Using bamboo (Bambusa vulgaris) as a field drainage material in ...

    African Journals Online (AJOL)

    user

    Bamboo (Bambusa vulgaris), one of the most widespread member of its genus, was ... climatic conditions and soil types (United Nations, 1972). ... concrete pipes are still being used as well. .... Tropical rain Forest zone with an annual rainfall of.

  7. Phenotypic Diversity among Croatian Common Bean (Phaseolus vulgaris L.) Landraces

    National Research Council Canada - National Science Library

    Monika Vidak; Sara Malešević; Martina Grdiša; Zlatko Šatović; Boris Lazarević; Klaudija Carović-Stanko

    2016-01-01

    Phenotypic diversity among Croatian common bean (Phaseolus vulgaris L.) landraces was assessed by analysing 12 qualitative and six quantitative traits in 338 accessions collected from all production areas in Croatia...

  8. Polyphosphate during the Regreening of Chlorella vulgaris under Nitrogen Deficiency

    National Research Council Canada - National Science Library

    Chu, Fei-Fei; Shen, Xiao-Fei; Lam, Paul K S; Zeng, Raymond J

    2015-01-01

    Polyphosphate (Poly-P) accumulation has been reported in Chlorella vulgaris under nitrogen deficiency conditions with sufficient P supply, and the process has been demonstrated to have great impact on lipid productivity...

  9. Caracterização imuno-histoquímica das alterações encefálicas em bovinos com hepatopatia tóxica por Senecio sp

    Directory of Open Access Journals (Sweden)

    Angelica T.B. Wouters

    2013-11-01

    Full Text Available Intoxicação por Senecio spp. é causa importante de doença e morte em bovinos no Rio Grande do Sul e frequentemente cursa com manifestações clínicas neurológicas e alterações histológicas encefálicas. Foi efetuada avaliação histológica em fígado e encéfalo de dez bovinos naturalmente intoxicados por Senecio sp. e foram realizadas as técnicas de histoquímica de ácido periódico de Schiff e de imuno-histoquímica, com emprego dos anticorpos anti-proteína fibrilar ácida (GFAP, anti-proteína S100 e anti-vimentina em amostras de telencéfalo, tronco encefálico e cerebelo. Na histologia do SNC observou-se vacuolização discreta a acentuada em substância branca e/ou junção entre substância branca e cinzenta, caracterizada como degeneração esponjosa. Na avaliação histoquímica e imuno-histoquímica não houve diferenças significativas em relação aos achados em encéfalo de onze bovinos controle, adultos, sem alterações hepáticas e/ou encefálicas, avaliados pelas mesmas técnicas.

  10. [Mapping of pathogenic genes in two families with autosomal dominant ichthyosis vulgaris].

    Science.gov (United States)

    Gong, Hui-Yong; Zhang, Jing; Hu, Zheng-Mao; Wu, Ling-Qian; Liang, De-Sheng; Xie, Zhi-Guo; Pan, Qian; Bu, Feng-Xiao; Peng, Yu; Xia, Kun; Xia, Jia-Hui

    2008-07-01

    To localize the pathogenic genes of autosomal dominant ichthyosis vulgaris, we ascertained two ichthyosis vulgaris families from Hunan Province. Venous blood samples were collected from affected and unaffected family members and genomic DNA was extracted. We then performed genome scan and linkage analysis using microsatellite markers around known ichthyosis vulgaris loci in chromosomes 1 and 10. In family 1, the locus linked to ichthyosis vulgaris was located near D1S498 (1q21), which overlapped with known ichthyosis vulgaris loci. In family 2, however, all known loci for ichthyosis vulgaris were excluded and the new locus remains to be identified.

  11. Potential Health Effects of Enzymatic Protein Hydrolysates from Chlorella vulgaris

    OpenAIRE

    2016-01-01

    Background and Objective: Chlorella vulgaris is a multi-cellular edible algal species with abundant proteins. Extraction of high value protein fractions for pharmaceutical and nutritional applications can significantly increase the commercial value of microalga biomasses. There is no known report on the anticancer peptides derived from the Chlorella vulgaris abundant protein.Materials and Methods: This study examined the antimicrobial and anticancer effects of peptides from a hydrolyzed Chlor...

  12. Ichthyosis Vulgaris Coexisted with Acrokeratosis Verruciformis: A Case Report

    Directory of Open Access Journals (Sweden)

    Gamze Serarslan

    2008-12-01

    Full Text Available Ichthyosis vulgaris is an autosomal dominant inherited, keratinization disorder and characterized by diffuse scaling. Acrokeratosis verruciformis is also an autosomal dominant, rare keratinization disorder and characterized by warty, brownish to skin colored papules on the dorsa of the hands and feet. We present a case of ichthyosis vulgaris coexisted with acrokeratosis verruciformis in a 24-year-old woman. (Turkderm 2008; 42: 134-6

  13. Lupus vulgaris of external nose--a case report.

    Science.gov (United States)

    Arunkumar, J S; Naveen, K N; Prasad, K C; Santhosh, S G; Hegde, J S

    2013-02-01

    Lupus vulgaris is the most common morphological variant of cutaneous tuberculosis accounting for approximately 59% of cases of cutaneous tuberculosis in India. We present a case of lupus vulgaris of external nose diagnosed early and treated with CAT-3 RNTCP regimen for six months without any nasal deformity except for a small scar over the dorsum of the nose. Patient followed up for one year after completion of the prescribed regimen, there being no recurrence of the lesion.

  14. Effects of processing on bean (Phaseolus vulgaris L.) protein quality.

    OpenAIRE

    Poel, van der, A.F.B.

    1990-01-01

    In animal production, feeding has an important impact on productivity and health of animals and feed composition is known to influence protein and energy metabolism directly. For monogastric animals complete diets are manufactured in which feed ingredients are used to supply the energy yielding and other nutrients. The common bean ( Phaseolus vulgaris L.) is such an ingredient.In common beans ( Phaseolus vulgaris L.) the supply of nutrients is often lower than is expected from its chemical an...

  15. Influence of diet in acne vulgaris and atopic dermatitis.

    Science.gov (United States)

    Shokeen, Divya

    2016-09-01

    Diet has been considered as an influence in dermatology for several years. Unfortunately, although correlation has been breached, causation is yet to be determined. Over the last couple years, a few reviews of the literature have been published regarding the influence of diet in acne vulgaris and atopic dermatitis. This article reviews some dietary restrictions and supplements that may have beneficial effects in managing patients with acne vulgaris and atopic dermatitis.

  16. Experimental poisoning by Senecio brasiliensis in calves: quantitative and semi-quantitative study on changes in the hepatic extracellular matrix and sinusoidal cells Intoxicação experimental por Senecio brasiliensis em bovinos: estudo quantitativo e semi-quantitativo da matrix extracelular e de células sinusoidais do fígado

    Directory of Open Access Journals (Sweden)

    Márcia Bersane A.M. Torres

    2008-01-01

    Full Text Available Extracellular matrix plays an important role in chronic hepatic lesions and has been studied in experimental intoxication models. However in cattle, studies on chronic disease have focused on the hepatocellular damage and extracellular matrix (ECM changes are usually overlooked. There are no specific studies on the hepatic ECM in either normal or chronically damaged bovine liver. Thus an experimental model of hepatic toxicity model using Senecio brasiliensis poisoned calves was designed. Senecio brasiliensis contains pyrrolizidine alkaloids which cause either acute or chronic progressive dose dependent liver damage. Five calves were orally fed with 0.38g of dry leaves of S. brasiliensis/kg/day for 24 days. Liver needle biopsy specimens were obtained every 15 days for 60 days. Clinical signs of digestive complications appeared at 3rd week. One calf died on 45th day and four were evaluated up to 60th day. Biopsy samples were processed for routine light microscopy, immuno-histochemistry and transmission electron microscopy. From 30th day on progressive liver damage characterized by hepatocellular ballooning, necrosis, apoptosis and megalocytosis, centrilobular, pericellular and portal fibrosis were seen by light microscopy. Quantitative and semi-quantitative measurements of hepatic ECM components were performed before and after the onset of lesions. Morphometric analysis of total collagen and elastic fiber system was conducted. Total collagen and I and III collagen types progressively increased in throughout the liver of affected calves. Changes in location, amount and disposition of the elastic fiber system were also observed. Then numbers of Kupffer cells were significantly increased at 30th day and total numbers of sinusoidal cells were significantly increased at 45th and 60th days. Liver damage was progressive and irreversible even after the exposure to the plant was discontinued. Severe fibrotic lesions occurred mainly in portal tracts

  17. Chlorella vulgaris: A Multifunctional Dietary Supplement with Diverse Medicinal Properties.

    Science.gov (United States)

    Panahi, Yunes; Darvishi, Behrad; Jowzi, Narges; Beiraghdar, Fatemeh; Sahebkar, Amirhossein

    2016-01-01

    Chlorella vulgaris is a green unicellular microalgae with biological and pharmacological properties important for human health. C. vulgaris has a long history of use as a food source and contains a unique and diverse composition of functional macro- and micro-nutrients including proteinsChlorella vulgaris is a green unicellular microalgae with biological and pharmacological properties important for human health. C. vulgaris has a long history of use as a food source and contains a unique and diverse composition of functional macro- and micro-nutrients including proteins, omega-3 polyunsaturated fatty acids, polysaccharides, vitamins and minerals. Clinical trials have suggested that supplementation with C. vulgaris can ameliorate amelioration hyperlipidemia and hyperglycemia, and protect against oxidative stress, cancer and chronic obstructive pulmonary disease. In this review, we summarize the findings on the health benefits of Chlorella supplementation and the molecular mechanisms underlying these effects., omega-3 polyunsaturated fatty acids, polysaccharides, vitamins and minerals. Clinical trials have suggested that supplementation with C. vulgaris can ameliorate amelioration hyperlipidemia and hyperglycemia, and protect against oxidative stress, cancer and chronic obstructive pulmonary disease. In this review, we summarize the findings on the health benefits of Chlorella supplementation and the molecular mechanisms underlying these effects.

  18. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations.

    Science.gov (United States)

    Satyal, Prabodh; Murray, Brittney L; McFeeters, Robert L; Setzer, William N

    2016-10-27

    Thyme (Thymus vulgaris L.) is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1) linalool; (2) borneol; (3) geraniol; (4) sabinene hydrate; (5) thymol; (6) carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography-mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%); the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%); the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%); and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%). A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris.

  19. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations

    Directory of Open Access Journals (Sweden)

    Prabodh Satyal

    2016-10-01

    Full Text Available Thyme (Thymus vulgaris L. is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1 linalool; (2 borneol; (3 geraniol; (4 sabinene hydrate; (5 thymol; (6 carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography–mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%; the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%; the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%; and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%. A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris.

  20. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations

    Science.gov (United States)

    Satyal, Prabodh; Murray, Brittney L.; McFeeters, Robert L.; Setzer, William N.

    2016-01-01

    Thyme (Thymus vulgaris L.) is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1) linalool; (2) borneol; (3) geraniol; (4) sabinene hydrate; (5) thymol; (6) carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography–mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%); the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%); the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%); and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%). A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris. PMID:28231164

  1. Changes in serum desnutrin levels in patients with acne vulgaris.

    Science.gov (United States)

    Demir, Betul; Ucak, Haydar; Cicek, Demet; Aydin, Suleyman; Erden, Ilker; Dertlioglu, Selma Bakar

    2014-01-01

    Androgens and insulin may contribute to increased sebum production in the pathogenesis of acne vulgaris. We investigated the association between serum desnutrin levels and acne vulgaris in the pathogenesis of insulin resistance. 25 patients presenting with acne vulgaris and 25 control subjects participated in this study. Fasting blood glucose, triglycerides, LDL, VLDL, HDL, total cholesterol, insulin, C-peptide and thyroid function tests were measured. The homeostasis model assessment of insulin resistance (HOMA-IR) was used to calculate insulin resistance. Desnutrin levels were determined by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's protocol. Patients with acne vulgaris had a mean serum desnutrin level of (8.83 ± 1.13 μIU/mL), which was statistically significantly lower in the control group (10:58 ± 3.43 μIU/mL). In patients with acne vulgaris the serum glucose levels, insulin levels and HOMA-IR values (87.92 ± 7:46 mg/dL, 11.33 ± 5.93 μIU/mL, 2.49 ± 1.40, respectively) were significantly higher than the control group (77.36 ± 9.83 mg/dL, 5.82 ± 2.68 μIU/mL, 1.11 ± 0.51, respectively) (p = 0.01, pacne vulgaris, as a result of increased levels of serum glucose and insulin, the function of desnutrin was suppressed, perhaps contributing to insulin resistance.

  2. Pemphigus Vulgaris and Infections: A Retrospective Study on 155 Patients

    Directory of Open Access Journals (Sweden)

    Nafiseh Esmaili

    2013-01-01

    Full Text Available Background. Autoimmune process and immunosuppressive therapy of pemphigus vulgaris would predispose the patients to infections. Aim. We aimed to study the prevalence of infection and pathogenic agents in pemphigus vulgaris patients admitted to dermatology service. Material and methods. This retrospective study was conducted on 155 pemphigus vulgaris patients (68 males, 87 females admitted to dermatology service between 2009 and 2011. In this study, the diagnosis of pemphigus vulgaris was confirmed by light microscopic and direct immunofluorescence findings. Data were collected through a questionnaire. Results. Of 155 pemphigus vulgaris patients, 33 had infection at admission and 9 acquired nosocomial infection. In addition, 37 cases of oral candidiasis and 15 cases of localized herpes simplex were recorded. Totally, 94 cases of infection were recorded. The occurrence of infection was significantly related to the severity of disease, number of hospital admissions, and presence of diabetes mellitus. The most common pathogenic germs isolated from cultures were Staphylococcus aureus and Escherichia coli. Conclusion. Severity of pemphigus vulgaris and diabetes were directly related with tendency to infections. Staphylococcus aureus and Escherichia coli were the most common pathogenic agents. Due to limitations of retrospective study, a prospective study is recommended.

  3. Acne vulgaris and rosacea: evaluation and management.

    Science.gov (United States)

    Webster, G F

    2001-01-01

    Acne vulgaris, commonly termed acne, is an extremely common disease. It can be found in nearly all teenagers to some degree as well as in women in their 30s. Regardless of severity, acne often has a greater psychologic effect than cutaneous effect. Indeed, most patients overestimate the severity of their disease, while most physicians underestimate its impact on their patients. Studies have shown that people with severe acne as teens are less employable as adults and that self-esteem is low. When combined with other adolescent tensions, acne can be a difficult disease to treat. Rosacea, which usually starts in the late 20s, may affect the eyes as well as the skin. This article describes the pathogenesis of acne and rosacea and treatment approaches the primary care physician can use.

  4. Ichthyosis vulgaris and pycnodysostosis: an unusual occurrence.

    Science.gov (United States)

    Kshirsagar, Vinayak Y; Ahmed, Minhajuddin; Nagarsenkar, Suhel; Sahoo, Kulmani; Shah, Kuldeep B

    2012-01-01

    Pycnodysostosis is a rare autosomal recessive disorder whose gene responsible for this phenotype (CTSK), mapped to human chromosome 1q21, code for the enzyme cathepsin K, a lysosomal cysteine protease; with an estimated incidence of 1.7 per 1 million births. This clinical entity includes micromelic dwarfism, increased radiological bone density, dysplasia of the skull, acro-osteolysis, straightening of the mandibular angle and in some cases, dysplasia of the acromial end of the clavicle. Oral and maxillo-facial manifestations of this disease are very clear. Herein we reported a case of pycnodysostosis, showing short stature with widening of the sutures, unfused anterior and posterior fontanelles, crowding of teeth with dental caries and typical radiological features associated with ichthyosis vulgaris and palmoplantar keratoderma.

  5. Pemphigus vulgaris: a multidisciplinary approach to management.

    Science.gov (United States)

    Vinall, Christopher; Stevens, Lucy; McArdle, Paul

    2013-12-16

    Pemphigus vulgaris (PV) is a rare but potentially life-threatening autoimmune disease affecting the mucosa and the skin. The disease is caused by circulating antibodies to desmosomes (important adhesion proteins linking cells together). Disruption of these intercellular connections results in a loss of cohesion between cells (acantholysis). The clinical result of this process is the development of multiple blisters that easily rupture, leaving behind painful sloughing eroded areas of mucosa and/or skin. We report a case of severe PV in a 56-year-old man presenting with widespread, painful, eroded mucocutaneous lesions. The severity of the disease demanded a range of medical and surgical specialties to successfully manage the problem. This paper highlights the importance of an early multidisciplinary team approach to improve the outcome of patients suffering with this disease.

  6. Plastid transformation in sugar beet: Beta vulgaris.

    Science.gov (United States)

    De Marchis, Francesca; Bellucci, Michele

    2014-01-01

    Chloroplast biotechnology has assumed great importance in the past 20 years and, thanks to the numerous advantages as compared to conventional transgenic technologies, has been applied in an increasing number of plant species but still very much limited. Hence, it is of utmost importance to extend the range of species in which plastid transformation can be applied. Sugar beet (Beta vulgaris L.) is an important industrial crop of the temperate zone in which chloroplast DNA is not transmitted trough pollen. Transformation of the sugar beet genome is performed in several research laboratories; conversely sugar beet plastome genetic transformation is far away from being considered a routine technique. We describe here a method to obtain transplastomic sugar beet plants trough biolistic transformation. The availability of sugar beet transplastomic plants should avoid the risk of gene flow between these cultivated genetic modified sugar beet plants and the wild-type plants or relative wild species.

  7. Pemphigus vulgaris-associated interstitial lung disease.

    Science.gov (United States)

    Bai, Yi-Xiu; Chu, Jin-Gang; Xiao, Ting; Chen, Hong-Duo

    2016-07-01

    Autoimmune bullous diseases (AIBDs)-associated interstitial lung disease (ILD) is extremely rare. Pemphigus vulgaris (PV) is an intraepidermal autoimmune blistering disease caused by circulating autoantibodies against desmoglein. To date, PV-associated ILD has rarely been reported in English literature. We report a rare association of PV and ILD. A 53-year-old Chinese female with PV for 8 months developed ILD after a relapse of PV for 2 months due to discontinuation of oral prednisone by herself. She was successfully treated by systemic methylprednisolone. Taken previously reported bullous pemphigoid-associated ILD and linear IgA/IgG bullous dermatosis-associated ILD together, in general, AIBDs-associated ILD occurs when AIBDs relapse or are not controlled, responds well to systemic corticosteroids, and has a relatively better prognosis when compared with rheumatoid arthritis- or dermatomyositis-associated ILD.

  8. Pregnancy-Associated "Cutaneous Type" Pemphigus Vulgaris.

    Science.gov (United States)

    Rangel, Javier

    2016-01-01

    The development of pemphigus, including pemphigus vulgaris (PV) and pemphigus foliaceus, during pregnancy is rare. PV manifests with mucosal and/or cutaneous erosions with flaccid bullae that are histologically characterized by suprabasilar acantholysis. In contrast, pemphigus foliaceus manifests with cutaneous-only involvement and superficial epidermal acantholysis. Enzyme-linked immunosorbent assay specific for autoantibodies against desmoglein 1 and desmoglein 3 aids in the diagnosis and differentiation between pemphigus subtypes. High-dose systemic corticosteroids are first-line agents in management of PV, yet their potential long-term use raises complex management issues associated with pregnancy and fetal risk. Here we report a rare case of cutaneous-limited PV in association with pregnancy.

  9. Setting the target for pemphigus vulgaris therapy

    Science.gov (United States)

    Ellebrecht, Christoph T.

    2017-01-01

    Despite the rising incidence of autoimmunity, therapeutic options for patients with autoimmune disease still rely on decades-old immunosuppressive strategies that risk severe and potentially fatal complications. Thus, novel therapeutic approaches for autoimmune diseases are greatly needed in order to minimize treatment-related toxicity. Such strategies would ideally target only the autoreactive immune components to preserve beneficial immunity. Here, we review how several decades of basic, translational, and clinical research on the immunology of pemphigus vulgaris (PV), an autoantibody-mediated skin disease, have enabled the development of targeted immunotherapeutic strategies. We discuss research to elucidate the pathophysiology of PV and how the knowledge afforded by these studies has led to the preclinical and clinical testing of targeted approaches to neutralize autoantibodies, to induce antigen-specific tolerance, and to specifically eliminate autoreactive B cells in PV. PMID:28289723

  10. Ichthyosis vulgaris and pycnodysostosis: An unusual occurrence

    Directory of Open Access Journals (Sweden)

    Vinayak Y. Kshirsagar

    2012-11-01

    Full Text Available Pycnodysostosis is a rare autosomal recessive disorder whose generesponsible for this phenotype (CTSK, mapped to human chromosome1q21, code for the enzyme cathepsin K, a lysosomal cysteineprotease; with an estimated incidence of 1.7 per 1 million births. This clinical entity includes micromelic dwarfism, increased radiological bone density, dysplasia of the skull, acro-osteolysis, straightening of the mandibular angle and in some cases, dysplasia of the acromial end of the clavicle. Oral and maxillo-facial manifestations of this disease are very clear. Herein we reported a case of pycnodysostosis, showing short stature with widening of the sutures, unfused anterior and posterior fontanelles, crowding of teeth with dental caries and typical radiological features associated with ichthyosis vulgaris and palmoplantar keratoderma.

  11. Construction of a full-length cDNA library for Senecio scandens%千里光全长cDNA文库的构建及分析

    Institute of Scientific and Technical Information of China (English)

    平军娇; 张珍; 蔡振锋; 汤贤春; 钱刚

    2012-01-01

    目的 构建千里光全长cDNA文库,以期研究千里光的功能基因组学信息,为克隆药理学性状相关的功能基因提供数据资源.方法 Trizol法提取千里光叶片总RNA,通过SMART(switching mechanism at 5’end of RNA transcript)构建全长cDNA文库,随机挑取600个单克隆测序分析文库滴度、全长率及冗余率,得到的EST序列进行Blast分析(NR、NT、Swiss-Prot、KEGG)及COG功能分类.结果 文库的库容为4.3×106 cfu/mL,插入片段大小平均1.7 kb,文库重组率96.35%,全长率58.24%,冗余率10.88%;获得524条全长EST序列,含有467条独立基因(unigenes),其中5条序列与千里光次生代谢产物的合成、运输与代谢有关.结论 经检测,SMART技术成功构建了千里光全长cDNA文库,该文库可用于千里光功能基因组鉴定、新基因筛选及次生代谢产物生物合成的表达调控研究.%Objective In the present study, our information from Senecio scandens full-length cDNA clones will serve as a useful resource for elucidating functional genes and will also aid a precise annotation of genomics in Compositae plants. Methods The total RNA was extracted from S. Scandens using Trizol method. SMART (switching mechanism at 5' end of RNA transcript) was applied to constructing the full-length cDNA library. Titer of the library, full-length ratio, and redundancy rate for 600 monoclone randomly selected sequencing library were evaluated by PCR amplification. NCBI and COG database was used to compare those sequences. Results Parameters of the the quality of cDNA library were as follows: the capacity of the library (4.3* 106 cfu/mL), the average size of the inserted fragment (1.7 kb), the recombination rate (96.35%), the full-length rate (58.24%), and the redundancy rate (10.88%). EST sequences for 524 full-length were obtained in this study, involving 467 unigenes, among which five sequences associated with synthesis, transport, and metabolism of S. Scandens secondary

  12. Genetics, chemistry and ecology of a qualitative glucosinolate polymorphism in Barbarea vulgaris

    NARCIS (Netherlands)

    Van Leur, H.

    2008-01-01

    Like many other plants, chemical defence compounds are involved in the defense of Barbarea vulgaris against natural enemies. Barbarea vulgaris produces glucosinolates, which are present in most crucifers such as cabbage, mustard, and the scientific model species Arabidopsis thaliana. Glucosinolates

  13. Genetics, chemistry and ecology of a qualitative glucosinolate polymorphism in Barbarea vulgaris

    NARCIS (Netherlands)

    Van Leur, H.

    2008-01-01

    Like many other plants, chemical defence compounds are involved in the defense of Barbarea vulgaris against natural enemies. Barbarea vulgaris produces glucosinolates, which are present in most crucifers such as cabbage, mustard, and the scientific model species Arabidopsis thaliana. Glucosinolates

  14. Salt Stress in Desulfovibrio vulgaris Hildenborough: An integratedgenomics approach

    Energy Technology Data Exchange (ETDEWEB)

    Mukhopadhyay, Aindrila; He, Zhili; Alm, Eric J.; Arkin, Adam P.; Baidoo, Edward E.; Borglin, Sharon C.; Chen, Wenqiong; Hazen, Terry C.; He, Qiang; Holman, Hoi-Ying; Huang, Katherine; Huang, Rick; Hoyner,Dominique C.; Katz, Natalie; Keller, Martin; Oeller, Paul; Redding,Alyssa; Sun, Jun; Wall, Judy; Wei, Jing; Yang, Zamin; Yen, Huei-Che; Zhou, Jizhong; Keasling Jay D.

    2005-12-08

    The ability of Desulfovibrio vulgaris Hildenborough to reduce, and therefore contain, toxic and radioactive metal waste has made all factors that affect the physiology of this organism of great interest. Increased salinity is an important and frequent fluctuation faced by D. vulgaris in its natural habitat. In liquid culture, exposure to excess salt resulted in striking elongation of D. vulgaris cells. Using data from transcriptomics, proteomics, metabolite assays, phospholipid fatty acid profiling, and electron microscopy, we used a systems approach to explore the effects of excess NaCl on D. vulgaris. In this study we demonstrated that import of osmoprotectants, such as glycine betaine and ectoine, is the primary mechanism used by D. vulgaris to counter hyperionic stress. Several efflux systems were also highly up-regulated, as was the ATP synthesis pathway. Increases in the levels of both RNA and DNA helicases suggested that salt stress affected the stability of nucleic acid base pairing. An overall increase in the level of branched fatty acids indicated that there were changes in cell wall fluidity. The immediate response to salt stress included up-regulation of chemotaxis genes, although flagellar biosynthesis was down-regulated. Other down-regulated systems included lactate uptake permeases and ABC transport systems. The results of an extensive NaCl stress analysis were compared with microarray data from a KCl stress analysis, and unlike many other bacteria, D. vulgaris responded similarly to the two stresses. Integration of data from multiple methods allowed us to develop a conceptual model for the salt stress response in D. vulgaris that can be compared to those in other microorganisms.

  15. Inter-laboratory comparison study for pyrrolizidine alkaloids in animal feed using spiked and incurred material.

    Science.gov (United States)

    de Nijs, Monique; Elbers, Ingrid J W; Mulder, Patrick P J

    2014-01-01

    Pyrrolizidine alkaloids (PAs) are hepatotoxic metabolites produced by plants. PAs in animal feed can cause acute or chronic intoxications in animals and can be transferred to milk. An inter-laboratory comparison study among 12 laboratories, using their own methods of analysis, was conducted for the detection and quantification of PAs in animal feed. The participants were asked to quantify PAs in a blank test sample, a blank test sample to be spiked with a provided spiking mixture of seven PA standards, and a test sample contaminated with common groundsel (Senecio vulgaris). Ten of the participating laboratories used an LC-MS/MS method, one used an LC-ToF-MS method, and one used a GC-MS method. None of the laboratories reported false-negative samples, while two laboratories reported false-positive results in the blank sample. z-scores were calculated for each laboratory for seven PAs in test samples B and C. z-scores varied considerably between laboratories for the concentrations of the free bases and less for the N-oxides, probably due to the lower levels of the free bases as compared with the N-oxides in the contaminated feed. Questionable or unsatisfactory results for the z-scores were obtained for 8% of the cases for the spiked sample and for 12% of the incurred sample. Three laboratories scored consequently positive or negative results. No preferred method for quantification of PAs in feed could be identified within the methods used for this study due to the relatively small number of participants. It was concluded that this inter-laboratory study shows that the methods used for PA detection need further development for accurate estimation of PAs in contaminated feed.

  16. Changes in Botrytis cinerea Conidia Caused by Berberis vulgaris Extract

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    Marcel PARVU

    2010-12-01

    Full Text Available Testing plant extracts for controlling fungal diseases is a main biocontrol method. More interesting is to see what happens to the fungus treated with the plant extract. Therefore, the aim of the study was to evaluate the antifungal activity of Berberis vulgaris extract on Botrytis cinerea and to examine the ultrastructural changes in B. cinerea conidia caused by the minimum inhibitory concentration (MIC, using SEM and TEM. The antifungal activity of B. vulgaris bark extract was investigated using agar dilution method, and compared to that of berberine. Fluconazole was used as the positive antimycotic control. It was found that (1 B. vulgaris bark extract had significant antifungal activity against B. cinerea, and its effect was stronger than that of pure berberine. It was also noted that (2B. vulgaris MIC caused severe structural changes of the conidia, comparable with berberine MIC effect; therefore (3 B. vulgaris bark extract might be recommended to be tested as a biocontrol agent against B. cinerea.

  17. [Lupus vulgaris manifestation as a destructive nose and facial tumor].

    Science.gov (United States)

    Haller, D; Reisser, C

    2009-04-01

    Lupus vulgaris is the most frequent manifestation of cutaneous tuberculosis, but in Europe it is limited to isolated cases. Mainly immunocompetent individuals are affected by this result of an endogenous reinfection on a lymphogenous-less frequently hematogenous-pathway. Lupus vulgaris has been observed to develop in more than 50% of all patients who already suffer from other manifestations of tuberculosis. The development of a squamous cell carcinoma in the lupus vulgaris is a rare complication; therefore, lupus vulgaris is deemed a facultative precancerosis.A 68-year-old female Serbo-Croatian patient presented with an extensive ulcerative nose and facial tumor. Her anamnesis included a squamous cell carcinoma of the nose that had been excised alio loco 3 years before. Further examinations revealed enlarged cervical lymphoma on both sides, and pulmonary metastases were also suspected. The tumor biopsy revealed a necrotic, granulomatous inflammation. No acid-fast rods were seen on Ziehl-Neelsen stain. The tuberculous origin of this ulcerative skin tumor-the lupus vulgaris-as an endogenous reinfection of pulmonary tuberculosis manifestation was confirmed by the detection of Mycobacterium tuberculosis DNA in polymerase chain reaction and the growth of Mycobacterium tuberculosis colonies in the bacterial culture (skin biopsy and bronchial secretion). The skin tumor as well as the pulmonary manifestation were successfully treated with combined tuberculostatic therapy and showed a dramatic response within 3 months.

  18. Acne vulgaris: diagnosis and management by the family physician

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    Ana Margarida Ferreira da Silva

    2014-01-01

    Full Text Available Acne vulgaris is the most common skin disease worldwide. It affects 85-100% of the population at any point in life. Consequently, it is a frequent reason for primary care visits. It usually begins at puberty, reaching its peak between the age of 14-17 in girls, and 16-19 in boys, and it is more severe and prevalent in males. Although widely discussed, Acne vulgaris still requires constant updating. We conducted a survey of clinical guidelines, systematic reviews, and meta-analyses published over the past 15 years, concerning acne vulgaris and its treatment. Acne vulgaris is usually divided in three clinical types: comedonal, papulopustular, and nodular. The diagnosis is based on clinical signs; however, other conditions should be considered. There are several pharmacological therapies available, especially retinoids, antimicrobials, and hormone therapy, which should be used after considering its indications (type of acne and its severity and side effects. Thus, the family physician plays a leading role in addressing acne vulgaris, from diagnosis to management of therapeutic options. 

  19. Gender-based variability in disease presentation in pemphigus vulgaris.

    Science.gov (United States)

    Naseer, Sahar Y; Gill, Liza; Shah, Jay; Sinha, Animesh A

    2014-10-01

    Pemphigus vulgaris is a chronic autoimmune blistering disorder of the skin. As with many autoimmune diseases, a female predominance in pemphigus vulgaris is well established. The genetic and physiological basis for this gender bias is not well understood. Moreover, it is unclear whether the affect of gender extends beyond disease susceptibility to influence disease presentation. To address this issue, we performed a comprehensive analysis of 72 male and 125 female pemphigus vulgaris patients across a set of defined demographic (HLA type, ethnicity) and clinical (age at disease onset, anti-desmoglein antibody levels, site of lesions, and history of autoimmune disease) factors. We find that male patients are more likely to present with disease onset before age 40 than females. Additionally, we find that males have increased cutaneous involvement and display greater co-expression of anti-Dsg1 and anti-Dsg3 antibodies, while females tend to have mucosal predominance and stronger personal and family histories of autoimmunity. We do not find any differences in the distribution of HLA type or ethnicity between male and female pemphigus vulgaris patients. Our findings establish that gender does influence disease presentation in pemphigus vulgaris, supporting a role for genetic and hormonal factors in immune dysregulation and perpetuation of the autoimmune phenotype.

  20. The complete chloroplast genome sequence of sugar beet (Beta vulgaris ssp. vulgaris).

    Science.gov (United States)

    Li, Han; Cao, Hua; Cai, Yan-Fei; Wang, Ji-Hua; Qu, Su-Ping; Huang, Xing-Qi

    2014-06-01

    The complete nucleotide sequence of the sugar beet (Beta vulgaris ssp. vulgaris) chloroplast genome (cpDNA) was determined in this study. The cpDNA was 149,637 bp in length, containing a pair of 24,439 bp inverted repeat regions (IR), which were separated by small and large single copy regions (SSC and LSC) of 17,701 and 83,057 bp, respectively. 53.4% of the sugar beet cpDNA consisted of gene coding regions (protein coding and RNA genes). The gene content and relative positions of 113 individual genes (79 protein encoding genes, 30 tRNA genes, 4 rRNA genes) were almost identical to those of tobacco cpDNA. The overall AT contents of the sugar beet cpDNA were 63.6% and in the LSC, SSC and IR regions were 65.9%, 70.8% and 57.8%, respectively. Fifteen genes contained one intron, while three genes had two introns.

  1. Treatment of acne vulgaris in pregnant patients.

    Science.gov (United States)

    Pugashetti, Rupa; Shinkai, Kanade

    2013-01-01

    The management of acne vulgaris in the setting of pregnancy raises important clinical considerations regarding the efficacy and safety of acne treatments in this special patient population. Particular challenges include the absence of safety data, discrepancy in safety data between different safety rating systems, and lack of evidence-based recommendations for the treatment of acne during pregnancy. Nonetheless, many therapeutic options exist, and the treatment of acne in pregnant women can be safely and often effectively accomplished. For mild or moderate disease, patients can be treated with topical antimicrobial agents, anti-inflammatory agents, as well as glycolic and salicylic acid. Several topical agents, notably benzoyl peroxide, previously viewed as potentially dangerous are cited by many sources as being considered safe. When necessary, systemic therapies that can be safely added include penicillins, amoxicillin, cephalosporins, erythromycin, clindamycin, and tetracyclines or sulfonamides, depending on the stage of fetal development. Adjunct therapy may include phototherapy or laser treatments. Physicians should work with this often highly motivated, safety-conscious patient population to tailor an individualized treatment regimen. This treatment regimen will likely shift throughout the different stages of fetal development, as distinct safety considerations are raised prior to conception as well as during each of the trimesters of pregnancy. Important considerations regarding acne management in breast-feeding mothers is also discussed.

  2. Topical and oral antibiotics for acne vulgaris.

    Science.gov (United States)

    Del Rosso, James Q

    2016-06-01

    Antibiotics, both oral and topical, have been an integral component of the management of acne vulgaris (AV) for approximately 6 decades. Originally thought to be effective for AV due to their ability to inhibit proliferation of Propionibacterium acnes, it is now believed that at least some antibiotics also exert anti-inflammatory effects that provide additional therapeutic benefit. To add, an increase in strains of P acnes and other exposed bacteria that are less sensitive to antibiotics used to treat AV have emerged, with resistance directly correlated geographically with the magnitude of antibiotic use. Although antibiotics still remain part of the therapeutic armamentarium for AV treatment, current recommendations support the following when used to treat AV: 1) monotherapy use should be avoided; 2) use benzoyl peroxide concomitantly to reduce emergence of resistant P acnes strains; 3) oral antibiotics should be used in combination with a topical regimen for moderate-to-severe inflammatory AV; and 4) use oral antibiotics over a limited duration to achieve control of inflammatory AV with an exit plan in place to discontinue their use as soon as possible. When selecting an oral antibiotic to treat AV, potential adverse effects are important to consider.

  3. Impaired water barrier function in acne vulgaris.

    Science.gov (United States)

    Yamamoto, A; Takenouchi, K; Ito, M

    1995-01-01

    In acne vulgaris, abnormal follicular keratinization is important for comedo formation, yet the precise mechanisms of comedogenesis are not known. The present study examined the interrelationship between sebum secretion rate (SSR), lipid content and water barrier function (WBF) of the stratum corneum (SC) in 36 acne patients and 29 control subjects. All major SC lipid classes were separated and quantified by thin-layer chromatography/photodensitometry. WBF was evaluated by measuring transepidermal water loss (TEWL), and the hygroscopic properties and waterholding capacity of the SC. The SSR over a period of 3 h was significantly higher in patients with moderate acne than in control subjects, but no significant difference was noticed between patients with mild acne and control subjects. Significant differences between patients with both moderate and mild acne and control subjects were noted in the amount of sphingolipids (ceramides and free sphingosine), but not for any other lipid classes. Furthermore in acne patients, lower amounts of sphingolipids were observed corresponding with a diminished WBF. These results suggest that an impaired WBF caused by decreased amounts of ceramides may be responsible for comedo formation, since barrier dysfunction is accompanied by hyperkeratosis of the follicular epithelium.

  4. Acne vulgaris in children and adolescents.

    Science.gov (United States)

    Schnopp, C; Mempel, M

    2011-08-01

    Acne vulgaris is a very common inflammatory skin disease originating from the pilosebaceous unit. Peak incidence is at puberty, but acne can affect all age groups. Prepubertal acne is rare, but important to recognize as diagnostic and therapeutic procedures differ from pubertal acne. Acne neonatorum is a mild, self-limiting disease, whereas acne infantum commonly presents with moderate to severe lesions and high risk of scarring thus requiring early intervention. Mid-childhood or prepubertal acne raises the suspicion of hyperandrogenemia, further investigations are indicated to rule out underlying disease. The same applies to any patient with very severe acne, acne not responding to therapy or unusual clinical presentation. Etiopathogenesis of acne is not yet fully understood. Familiy history is the most important risk factor to develop severe acne and scarring. The relevance of life style factors such as smoking or diet is controversial. Lately high carbohydrate diet and dairy products have been implicated as aggravating factors. Mild acne normally responds to topical monotherapy, in moderate disease combination of two synergistically acting substances (e.g. benzoyl peroxid plus antibiotic, benzoyl peroxid plus retinoid, retinoid plus antibiotic, benzoyl peroxid plus azelaic acid) will improve clinical response. Retinoids and/or benzoylperoxid have been shown to be effective in maintenance therapy. In patients with severe disease or high risk of scarring systemic therapy with antibiotics, oral contraceptives with antiandrogenic properties and in particularly isotretinoin as most effective acne treatment should be considered early to avoid physical and emotional scars.

  5. Lupus vulgaris in a pediatric patient: a clinicohistopathological diagnosis.

    Science.gov (United States)

    Afsar, F Sule; Afsar, Ilhan; Diniz, Gulden; Asilsoy, Suna; Sorguc, Yelda

    2008-04-01

    Lupus vulgaris is the most common form of cutaneous tuberculosis which usually occurs in patients previously sensitized to Mycobacterium tuberculosis. We present a case of a 10-year-old boy who was diagnosed as lupus vulgaris clinically and histopathologically. He had well demarcated, irregularly bordered, pink, infiltrated plaques on his left cheek showing apple-jelly appearance on diascopy. The histopathological examination showed tuberculoid granulomas with Langhans type giant cells. The Mantoux reactivity was in normal limits, and no acid-fast bacilli was found in the lesion, either by direct stained smears or by culture. The lesions showed marked improvement on anti-tuberculosis treatment. We want to emphasize that histopathological examination has diagnostic value in lupus vulgaris in correlation with clinical appearance, when direct analysis or culture is negative.

  6. Lupus vulgaris occurring in a locus minoris resistentiae.

    Science.gov (United States)

    Long, Richard; Beatch, Anita; Lee, Mao-Cheng; Cheung-Lee, Melody; Wasel, Norman

    2009-01-01

    The pathogenesis of lupus vulgaris, a form of cutaneous tuberculosis, is not always clear, especially in patients who do not have coexistent extracutaneous tuberculosis and in patients with single lesions. To report a case of lupus vulgaris in a locus minoris resistentiae (a site of reduced resistance) and to use a unique set of clinical circumstances and laboratory tests to reconstruct the pathogenesis of the lesion and the response to treatment. Lupus vulgaris can occur in a locus minoris resistentiae; local trauma and possibly other factors, such as increased temperature, topical corticosteroids, and the virulence of the infecting strain, may facilitate the growth of Mycobacterium tuberculosis present at a locus minoris resistentiae as a result of a silent bacillemia.

  7. Lupus vulgaris in a pediatric patient: a clinicohistopathological diagnosis

    Directory of Open Access Journals (Sweden)

    F. Sule Afsar

    2008-04-01

    Full Text Available Lupus vulgaris is the most common form of cutaneous tuberculosis which usually occurs in patients previously sensitized to Mycobacterium tuberculosis. We present a case of a 10-year-old boy who was diagnosed as lupus vulgaris clinically and histopathologically. He had well demarcated, irregularly bordered, pink, infiltrated plaques on his left cheek showing apple-jelly appearance on diascopy. The histopathological examination showed tuberculoid granulomas with Langhans type giant cells. The Mantoux reactivity was in normal limits, and no acid-fast bacilli was found in the lesion, either by direct stained smears or by culture. The lesions showed marked improvement on anti-tuberculosis treatment. We want to emphasize that histopathological examination has diagnostic value in lupus vulgaris in correlation with clinical appearance, when direct analysis or culture is negative.

  8. The innate immunity in the cnidarian Hydra vulgaris

    Directory of Open Access Journals (Sweden)

    B Altincicek

    2009-08-01

    Full Text Available Hydra vulgaris is currently receiving increased attention as a genetically tractable invertebrate model system for studying important processes of life such as the innate immune defense. Similar to complex animals, H. vulgaris polyps respond to injury by abrupt muscle contraction, by limited escape behavior, and by healing the damaged tissue. Simultaneously, cellular processes such as phagocytosis and programmed cell death as well as the massive production of antimicrobial peptides are induced. Recent studies identified several molecular pathways controlling these responses; however, the interdependence of innate immunity and, for example, regeneration and tissue remodeling is not well elucidated yet. H. vulgaris belongs to the Cnidaria representing the phylogenic sister group of bilaterian animals; hence, a better understanding of evolutionarily conserved as well as Hydra/Cnidaria-specific immune responses will provide deep insight into both origin and evolution of the animal innate immune system

  9. Recycling of food waste as nutrients in Chlorella vulgaris cultivation.

    Science.gov (United States)

    Lau, Kin Yan; Pleissner, Daniel; Lin, Carol Sze Ki

    2014-10-01

    Heterotrophic cultivation of Chlorella vulgaris was investigated in food waste hydrolysate. The highest exponential growth rate in terms of biomass of 0.8day(-1) was obtained in a hydrolysate consisting of 17.9gL(-1) glucose, 0.1gL(-1) free amino nitrogen, 0.3gL(-1) phosphate and 4.8mgL(-1) nitrate, while the growth rate was reduced in higher concentrated hydrolysates. C. vulgaris utilized the nutrients recovered from food waste for the formation of biomass and 0.9g biomass was produced per gram glucose consumed. The microalgal biomass produced in nutrient sufficient batch cultures consisted of around 400mgg(-1) carbohydrates, 200mgg(-1) proteins and 200mgg(-1) lipids. The conversion of nutrients derived from food waste and the balanced biomass composition make C. vulgaris a promising strain for the recycling of food waste in food, feed and fuel productions.

  10. Timely recognition of pemphigus vulgaris by dental professionals.

    Science.gov (United States)

    Fatahzadeh, Mahnaz

    2013-07-01

    Pemphigus vulgaris is a potentially fatal mucocutaneous, vesiculobullous disorder of autoimmune etiology. Regrettably, affected patients frequently experience considerable discomfort and diagnostic delay for months in spite of multiple clinician contacts. The high likelihood of disease manifestations in the oral cavity and serious nature of potential oral and systemic complications mandates dental professionals to recognize early oral signs and symptoms of pemphigus vulgaris and contribute to timely diagnosis and medical intervention to prevent disease progression. This case report presents a young man with pemphigus vulgaris whose oral disease caused him significant suffering and spread to the skin before he was finally diagnosed 3 months after the onset. The signs and symptoms of the disease are reviewed and the potential role of dental providers in timely recognition and management is emphasized.

  11. Autoreactive T cells in the immune pathogenesis of pemphigus vulgaris.

    Science.gov (United States)

    Amber, Kyle T; Staropoli, Patrick; Shiman, Michael I; Elgart, George W; Hertl, Michael

    2013-11-01

    Pemphigus vulgaris is a life-threatening autoimmune blistering disease caused by anti-desmoglein IgG autoantibodies that finally lead to acantholysis presenting clinically as progressive blistering. Whilst the production of pathogenic antibodies is key to the development of pemphigus vulgaris, many immunological steps are required prior to autoantibody induction. We review advances in the understanding of these immunologic processes with a focus on human leucocyte antigen polymorphisms and antigen recognition, epitope spreading, central and peripheral tolerance, T helper differentiation, induction of pro- and anti-inflammatory cytokines and T-cell regulation of B cells. Targeting autoaggressive T cells as regulators and stimulators of B-cell antibody production should allow for more specific therapeutic immune interventions, avoiding the global immunosuppression seen with many commonly used immunosuppressants in pemphigus vulgaris.

  12. Respuesta de Phaseolus vulgaris a microorganismos promotores de crecimiento vegetal

    Directory of Open Access Journals (Sweden)

    Violeta Edith Romero - García

    2016-01-01

    Full Text Available El objetivo de esta investigaci ón fue analizar la respuesta de P. vulgaris a la inoculación individual y consorcio de MPCV a dosis reducida al 50% del FN. Para ello se inoculó P. vulgaris individualmente con: B. cereus, R. etli y T. harzianum y en consorcio de M PCV en jarras de Leonard tratada c on NH 4 NO 3 al 50 %, bajo un diseño experimental de bloques al azar; con variables respuestas: porcentaje y días a germinación, altura de planta y longitud radical, peso fresco/seco aéreo y radical (PFA/PSA/PFR/PSR a plántula y floración; los datos experimentales se analizaron por Tukey , p ≤ 0 , 05. Los resultados mostraron que P. vulgaris inoculado con R. etli y T. harzianum tuvo un 100 % y 95,8% de germinación o emergencia en 4,8 y 4 , 5 días, respectivamente, valores con diferencia estadística en comparación con el 91,7% en 7,17 días en P. vu lgaris sin inocular con el 100% de FN o testigo fertilizado (TF. En la etapa de plántula de P. vulgaris con T. harzianum y R. etli fue de 1,12 g y 0,72 g PSA, así como de 0,31 g y 0,21 de PSR, respectivamente , superior a P. vulgaris (TF de 0,52 g de PSA y 0,19 g de PSR. Lo anterior apoya el potencial de R. etli y T. harzianum para la producción de P. vulgaris a dosis reducida de FN, comparado con el efecto positivo individual de B. cereus y en consorcio en la misma leguminosa .

  13. Transcriptome analysis of the Octopus vulgaris central nervous system.

    Directory of Open Access Journals (Sweden)

    Xiang Zhang

    Full Text Available BACKGROUND: Cephalopoda are a class of Mollusca species found in all the world's oceans. They are an important model organism in neurobiology. Unfortunately, the lack of neuronal molecular sequences, such as ESTs, transcriptomic or genomic information, has limited the development of molecular neurobiology research in this unique model organism. RESULTS: With high-throughput Illumina Solexa sequencing technology, we have generated 59,859 high quality sequences from 12,918,391 paired-end reads. Using BLASTx/BLASTn, 12,227 contigs have blast hits in the Swissprot, NR protein database and NT nucleotide database with E-value cutoff 1e(-5. The comparison between the Octopus vulgaris central nervous system (CNS library and the Aplysia californica/Lymnaea stagnalis CNS ESTs library yielded 5.93%/13.45% of O. vulgaris sequences with significant matches (1e(-5 using BLASTn/tBLASTx. Meanwhile the hit percentage of the recently published Schistocerca gregaria, Tilapia or Hirudo medicinalis CNS library to the O. vulgaris CNS library is 21.03%-46.19%. We constructed the Phylogenetic tree using two genes related to CNS function, Synaptotagmin-7 and Synaptophysin. Lastly, we demonstrated that O. vulgaris may have a vertebrate-like Blood-Brain Barrier based on bioinformatic analysis. CONCLUSION: This study provides a mass of molecular information that will contribute to further molecular biology research on O. vulgaris. In our presentation of the first CNS transcriptome analysis of O. vulgaris, we hope to accelerate the study of functional molecular neurobiology and comparative evolutionary biology.

  14. Pemphigus vulgaris in a pregnant woman and her neonate

    Science.gov (United States)

    Ibrahim, Sameera Begum Kader; BM, Yashodhara; Umakanth, Shashikiran; Kanagasabai, Sachchithanatham

    2012-01-01

    A 23-year-old pregnant woman in her second trimester of pregnancy presented with blisters on the face, abdomen and the leg. Based on the clinical presentation and skin biopsy (histopathology and direct immunofluorescence) the diagnosis of pemphigus vulgaris was established. The child born to this patient also had similar skin lesions. The lesions in the mother and the child improved after treatment. The authors report a rare case of pemphigus vulgaris in a pregnant lady and neonatal pemphigus in her child, both of whom were treated successfully. PMID:22744241

  15. [Mutations in the gene encoding filaggrin cause ichthyosis vulgaris].

    Science.gov (United States)

    Prasad, Sumangali Chandra; Rasmussen, Kirsten; Bygum, Anette

    2011-02-14

    Ichthyosis vulgaris is a common genetic skin disorder with an estimated prevalence of 1:250 caused by mutations in the gene encoding filaggrin. This disorder manifests itself within the first year of life and is clinically characterized by dry, scaly skin, keratosis pilaris, palmar hyperlinearity and atopic manifestations. Patients with a severe phenotype are homozygous or compound heterozygous for the mutations, whereas heterozygous patients show mild disease, suggesting semidominant inheritance with incomplete penetrance. We present a patient with classic severe ichthyosis vulgaris, atopic eczema and two loss-of-function mutations.

  16. Coincident systemic lupus erythematosus and psoriasis vulgaris: a case report.

    Science.gov (United States)

    Wang, Y; Da, G; Yu, Y; Han, J; Li, H

    2015-12-01

    Psoriasis vulgaris is an autoimmune chronic inflammatory skin disease, but its association with other typical autoimmune disease such as systemic lupus erythematosus has only occasionally been reported. We presented a 25-year-old female who developed systemic lupus erythematosus associated with psoriasis vulgaris. Her conditions were in good control after she got administration of prednisolone (5 mg/day) and Tripterygium Wilfordii Hook (20 mg/day). It is necessary to integrate past history and physical examination to diagnose coincident SLE and psoriasis, and combined treatment with prednisolone and Tripterygium Wilfordii Hook proves effective.

  17. The dual nature of Interleukin-10 in pemphigus vulgaris

    Science.gov (United States)

    Cho, Michael Jeffrey; Ellebrecht, Christoph T.; Payne, Aimee S.

    2014-01-01

    The immunomodulatory cytokine interleukin-10 (IL-10) plays beneficial but also potentially detrimental roles in inflammation, infection, and autoimmunity. Recent studies suggest a regulatory role for IL-10-expressing B cells in the autoimmune blistering disease pemphigus vulgaris. Here we review the studies on IL-10 in pemphigus vulgaris and discuss the potential pathophysiological significance of these findings in comparison to prior studies of IL-10 in other human conditions. A better understanding of the complex roles of IL-10 in immune regulation may improve our understanding of pemphigus pathogenesis and treatment. PMID:25464924

  18. Novel protocol for lutein extraction from microalga Chlorella vulgaris

    DEFF Research Database (Denmark)

    D'Este, Martina; De Francisci, Davide; Angelidaki, Irini

    2017-01-01

    Lutein is a pigment generally extracted from marigold flowers. However, lutein is also found in considerable amounts in microalgae. In this study a novel method was developed to improve the extraction efficiency of lutein from microalga C. vulgaris. Differently from conventional methods, ethanol...... was used instead of water in the saponification step, which was conducted simultaneously to the solvent extraction, performed using dichloromethane. The amount of lutein extracted from C. vulgaris dried biomass increased more than threefold, from 0.20 ± 0.00 mgLutein/gDM to 0.69 ± 0.08 mgLutein/gDM. Lutein...

  19. Acne Vulgaris and Acne Rosacea: An Update in Etiopathogenesis

    Directory of Open Access Journals (Sweden)

    Ozlem Ekiz

    2014-04-01

    Full Text Available Acne vulgaris is a chronic inflammatory disease of the pilosebaceous unit, characterized by comedones, papules, pustules, nodules, and scars rarely. The major pathogenic factors are abnormal follicular differentiation and increased ductal cornification, abnormal activity of sebaceous glands, microbial colonization of pilosebaceous units by Propionibacterium acnes and inflammation. Rosacea is a common, chronic inflammatory relapsing skin disorder of the central area of the face characterized by transient or persistent erythema, telangiectasia, papules and pustules. Although several hypotheses have been suggested for the etiopathogenesis of rosacea, the exact etiology is still unknown. In this review, we tried to summarize up-to-date information about etiopathogenesis of acne vulgaris and rosocea.

  20. The dual nature of interleukin-10 in pemphigus vulgaris.

    Science.gov (United States)

    Cho, Michael Jeffrey; Ellebrecht, Christoph T; Payne, Aimee S

    2015-06-01

    The immunomodulatory cytokine interleukin-10 (IL-10) plays beneficial but also potentially detrimental roles in inflammation, infection, and autoimmunity. Recent studies suggest a regulatory role for IL-10-expressing B cells in the autoimmune blistering disease pemphigus vulgaris. Here we review the studies on IL-10 in pemphigus vulgaris and discuss the potential pathophysiological significance of these findings in comparison to prior studies of IL-10 in other human conditions. A better understanding of the complex roles of IL-10 in immune regulation may improve our understanding of pemphigus pathogenesis and treatment.

  1. Management of pemphigus vulgaris during acute phase

    Directory of Open Access Journals (Sweden)

    Kar P

    2003-03-01

    Full Text Available We present our experience with 21 patients of pemphigus vulgaris seen over a period of 10 years managed in service hospitals during acute phase of the disease. Age groups of patients ranged from 25-45 years. Eighteen (85.7% were young adults, 30-40 years of age. Fifteen (71.4% were men and 6(28.6% were women. All the cases were hospitalized in ICU, till the acute phase of the disease subsided. Complete hematological profile, urinalysis, serum biochemistry and repeated bacterial cultures from the skin were carried out in all patients at the time of admission and thereafter weekly. The treatment comprised of potassium permanganate lotion bath (1:10000 and 1 framycetin gauze dressing of the denuded areas, maintenance of fluid and electrolyte balance. All suspected infections and septicemia were treated with appropriate antibiotics. The corticosteroids were usually administered as a single dose of prednisolone 1 mg/kg/day. Cyclophosphamide was given at an initial dose of 50mg/day and the dose was escalated to 100mg/day. Once the bulk of the lesions were healed, the dose of corticosteroids was gradually lowered by approximately 50% every two weeks and cyclophosphamide was continued till patients were symptomfree. Out of 21 patients receiving corticosteroids, cyclophosphamide and other supportive therapy, 20(95% had undergone clinical resolution of the disease. During follow up study 15(71.4% patients remained symptom-free and undergone clinical remission. Five patients (23.8% had relapse, out of which 4(19% remained symptom free, after subsequent treatment. There was one death (4.7% in our study.

  2. Differential expression patterns of non-symbiotic hemoglobins in sugar beet (Beta vulgaris ssp. vulgaris).

    Science.gov (United States)

    Leiva-Eriksson, Nélida; Pin, Pierre A; Kraft, Thomas; Dohm, Juliane C; Minoche, André E; Himmelbauer, Heinz; Bülow, Leif

    2014-04-01

    Biennial sugar beet (Beta vulgaris spp. vulgaris) is a Caryophyllidae that has adapted its growth cycle to the seasonal temperature and daylength variation of temperate regions. This is the first time a holistic study of the expression pattern of non-symbiotic hemoglobins (nsHbs) is being carried out in a member of this group and under two essential environmental conditions for flowering, namely vernalization and length of photoperiod. BvHb genes were identified by sequence homology searches against the latest draft of the sugar beet genome. Three nsHb genes (BvHb1.1, BvHb1.2 and BvHb2) and one truncated Hb gene (BvHb3) were found in the genome of sugar beet. Gene expression profiling of the nsHb genes was carried out by quantitative PCR in different organs and developmental stages, as well as during vernalization and under different photoperiods. BvHb1.1 and BvHb2 showed differential expression during vernalization as well as during long and short days. The high expression of BvHb2 indicates that it has an active role in the cell, maybe even taking over some BvHb1.2 functions, except during germination where BvHb1.2 together with BvHb1.1-both Class 1 nsHbs-are highly expressed. The unprecedented finding of a leader peptide at the N-terminus of BvHb1.1, for the first time in an nsHb from higher plants, together with its observed expression indicate that it may have a very specific role due to its suggested location in chloroplasts. Our findings open up new possibilities for research, breeding and engineering since Hbs could be more involved in plant development than previously was anticipated.

  3. Bioremediation of the textile waste effluent by Chlorella vulgaris

    Directory of Open Access Journals (Sweden)

    Hala Yassin El-Kassas

    2014-01-01

    Full Text Available The microalgae biomass production from textile waste effluent is a possible solution for the environmental impact generated by the effluent discharge into water sources. The potential application of Chlorella vulgaris for bioremediation of textile waste effluent (WE was investigated using 22 Central Composite Design (CCD. This work addresses the adaptation of the microalgae C. vulgaris in textile waste effluent (WE and the study of the best dilution of the WE for maximum biomass production and for the removal of colour and Chemical Oxygen Demand (COD by this microalga. The cultivation of C. vulgaris, presented maximum cellular concentrations Cmax and maximum specific growth rates μmax in the wastewater concentration of 5.0% and 17.5%, respectively. The highest colour and COD removals occurred with 17.5% of textile waste effluent. The results of C. vulgaris culture in the textile waste effluent demonstrated the possibility of using this microalga for the colour and COD removal and for biomass production. There was a significant negative relationship between textile waste effluent concentration and Cmax at 0.05 level of significance. However, sodium bicarbonate concentration did not significantly influence the responses of Cmax and the removal of colour and COD.

  4. Separation of Chlorella vulgaris from liquid phase using bioflocculants

    Directory of Open Access Journals (Sweden)

    Gizem Günay

    2014-12-01

    results showed that C. vulgaris was partially separated from the liquid phase. However, the experiments will continue for the purpose of increasing the flocculating activity. Getting successfully experimental results with kaolin showed that bioflocculant has a potential use in wastewater treatment. For this reason, it also is thought to analyze the effect of bioflocculant on the wastewater treatment with further studies.[¤

  5. Acne vulgaris: A review of causes and treatment options.

    Science.gov (United States)

    Well, Danielle

    2013-10-10

    Acne vulgaris is a disorder of the sebaceous follicle. The cause is multifactorial, and both adolescents and adults can be affected. Acne is associated with a significant financial burden and considerable psychological distress. Treatment options are reviewed, including over-the-counter medications, prescription medications, and in-office procedures.

  6. [Molecular genetic investigation of sugar beet (Beta vulgaris L.)].

    Science.gov (United States)

    Butorina, A K; Kornienko, A V

    2011-10-01

    Molecular genetic studies of sugar beet (Beta vulgaris L.) are reviewed as a basis for the development of genomics of this species. The methods used to study structural and functional genomics are considered. The results and their application to increase the efficiency of sugar beet breeding are discussed.

  7. Dapsone 7.5% Gel: A Review in Acne Vulgaris.

    Science.gov (United States)

    Al-Salama, Zaina T; Deeks, Emma D

    2017-02-01

    Dapsone 7.5% gel (Aczone(®)) is indicated for the once-daily topical treatment of acne vulgaris in patients aged ≥12 years. Dapsone is a sulfone antibacterial with anti-inflammatory actions, which are thought to be largely responsible for its efficacy in treating acne vulgaris. In two phase III trials of 12 weeks' duration in patients aged ≥12 years with moderate acne vulgaris, once-daily dapsone 7.5% gel reduced acne severity (as per the Global Acne Assessment Score) and lesion counts versus vehicle. The benefits of dapsone 7.5% gel over vehicle were seen as early as week 2 for inflammatory lesion counts, and from week 4 or 8 for other outcomes. Dapsone 7.5% gel was well tolerated, with a low incidence of treatment-related adverse events, with the majority of adverse events being administration-site related and mild or moderate in severity. Thus, dapsone 7.5% gel is an effective and well tolerated option for the topical treatment of acne vulgaris in patients aged ≥12 years, with the convenience of once-daily application.

  8. Gallium-67-citrate uptake in a case of acne vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Kipper, M.S.; Taylor, A.; Ashburn, W.L.

    1981-09-01

    A case of increased Ga-67 uptake in a patient with active acne vulgaris is reported. The scan was requested in a search for metastatic testicular carcinoma or bleomycin pulmonary toxicity. Careful clinical evaluation including physical examination was necessary in order to avoid an erroneous scan interpretation.

  9. An update on the management of acne vulgaris

    Directory of Open Access Journals (Sweden)

    Jonette Keri

    2009-06-01

    Full Text Available Jonette Keri1,2, Michael Shiman11Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USA; 2Dermatology Service, Miami VA Hospital, FL, USAAbstract: Acne vulgaris is a common skin disorder that can affect individuals from childhood to adulthood, most often occurring in the teenage years. Acne can have a significant physical, emotional, and social impact on an individual. Many different treatment options are available for the treatment of acne vulgaris. Commonly used topical treatments include benzoyl peroxide, antibiotics, sulfur and sodium sulfacetamide, azelaic acid, and retinoids. Systemic treatment is frequently used and includes the use of systemic antibiotics, oral contraceptives, antiandrogens, and retinoids. Other treatment modalities exist such as the use of superficial chemical peels as well as using laser and light devices for the treatment of acne. With the multitude of treatment options and the rapidly expanding newer technologies available to clinicians, it is important to review and be aware of the current literature and studies regarding the treatment of acne vulgaris.Keywords: acne vulgaris, treatment, benzoyl peroxide, antibiotics, retinoids, lasers

  10. Vegetative propagation of Syringa vulgaris L. in vitro.

    NARCIS (Netherlands)

    Pierik, R.L.M.; Steegmans, H.H.M.; Elias, A.A.; Stiekema, O.T.J.; Velde, van der A.J.

    1988-01-01

    Excised shoot tips from adult Syringa vulgaris L. plants were rejuvenated by repeated subculturing in vitro. The number of subcultures required to rejuvenate the shoots was strongly dependent on the age and genotype of the plant material. Three rootstocks (K8, A2 and A3) and 5 cultivars (Mademoisell

  11. Evaluation of common bean (Phaseolus vulgaris) response to charcoal rot

    Science.gov (United States)

    Charcoal rot in common beans (Phaseolus vulgaris L.), caused by Macrophomina phaseolina (Tassi) Gold. (Mph), is an endemic disease in the prevailing hot and dry conditions in southern Puerto Rico. This study evaluated the 120 bean genotypes that compose the BASE 120 panel under screenhouse conditio...

  12. Pemphigus Vulgaris Activity Score and Assessment of Convergent Validity

    Directory of Open Access Journals (Sweden)

    Cheyda Chams-Davatchi

    2013-04-01

    Full Text Available Pemphigus is a rare autoimmune blistering disease with different phenotypes. The evaluation of therapeutic interventions requires a reliable, valid and feasible to use measurement. However, there is no gold standard to measure the disease activity in clinical trials. In this study we aimed to introduce the pemphigus vulgaris activity score (PVAS measurement and to assess the convergent validity with the experts’ opinion of disease activity. In PVAS scoring, the distribution of pemphigus vulgaris antigen expression in different anatomical regions is taking in to account with special consideration of the healing process. PVAS is a 0-18 scale, based on the extent of mucocutaneous involvement, type of lesion and the presence of Nikolsky’s sign. The sum of the scores of total number of lesions, number of different anatomic regions involvement and Nikolsky’s sign is weighted by the type of lesion. In the present study, PVAS was assessed in 50 patients diagnosed with pemphigus vulgaris by one dermatologist. Independently, five blinded experts scored all the patients through physician’s global assessment (PGA. The convergent validity with experts’ opinion was assessed. The Spearman coefficient of correlation showed the acceptable value of 0.751 (95%CI: 0.534- 0.876. PVAS is a valid, objective and simple-to-use scoring measurement. It showed a good correlation with PGA of pemphigus disease activity in Iranian patients with pemphigus vulgaris

  13. Variation in Breeding Systems in Hypericum Perforatum and Prunella Vulgaris

    Science.gov (United States)

    The effective conservation of new crop germplasm and its efficient use in new-crop development both rely on a clear understanding of the crop's reproductive biology. Hypericum perforatum (St. John's wort) and Prunella vulgaris (Common selfheal) are two medicinal plant species with potential for crop...

  14. Pemphigus vulgaris activity score and assessment of convergent validity.

    Science.gov (United States)

    Chams-Davatchi, Cheyda; Rahbar, Ziba; Daneshpazhooh, Maryam; Mortazavizadeh, Seyed Mohammad Ali; Akhyani, Maryam; Esmaili, Nafiseh; Balighi, Kamran

    2013-05-07

    Pemphigus is a rare autoimmune blistering disease with different phenotypes. The evaluation of therapeutic interventions requires a reliable, valid and feasible to use measurement. However, there is no gold standard to measure the disease activity in clinical trials. In this study we aimed to introduce the pemphigus vulgaris activity score (PVAS) measurement and to assess the convergent validity with the experts' opinion of disease activity. In PVAS scoring, the distribution of pemphigus vulgaris antigen expression in different anatomical regions is taking in to account with special consideration of the healing process. PVAS is a 0-18 scale, based on the extent of mucocutaneous involvement, type of lesion and the presence of Nikolsky's sign. The sum of the scores of total number of lesions, number of different anatomic regions involvement and Nikolsky's sign is weighted by the type of lesion. In the present study, PVAS was assessed in 50 patients diagnosed with pemphigus vulgaris by one dermatologist. Independently, five blinded experts scored all the patients through physician's global assessment (PGA). The convergent validity with experts' opinion was assessed. The Spearman coefficient of correlation showed the acceptable value of 0.751 (95%CI: 0.534- 0.876). PVAS is a valid, objective and simple-to-use scoring measurement. It showed a good correlation with PGA of pemphigus disease activity in Iranian patients with pemphigus vulgaris.

  15. Anaphylaxis to pine nut: cross-reactivity to Artemisia vulgaris?

    Science.gov (United States)

    Rodrigues-Alves, R; Pregal, A; Pereira-Santos, M C; Branco-Ferreira, M; Lundberg, M; Oman, H; Pereira-Barbosa, M

    2008-01-01

    The use of pine nuts, the seeds of Pinus pinea, is on the increasing in the modern Mediterranean diet. Little more than 20 cases of allergy to this tree nut have been published, and cross-reactivity with pine pollen, peanut and almond has already been reported. We describe the case of a young boy with several episodes of anaphylaxis after pine nut ingestion. Specific IgE to pine nut and Artemisia vulgaris was demonstrated by skin prick tests and in vitro determination of specific IgE, although no IgE to pine pollen or other nuts was detected. Immunoblotting of Artemisia vulgaris and pine nut revealed two matching diffuse bands, just below 14 kDa and 30 kDa. The ImmunoCAP inhibition assays showed complete inhibition of pine nut specific IgE after serum incubation with Artemisia vulgaris extract. As far as we know, this is the first reported case of documented cross-reactivity between pine nut and Artemisia vulgaris.

  16. The epidemiology of acne vulgaris in late adolescence

    Directory of Open Access Journals (Sweden)

    Lynn DD

    2016-01-01

    Full Text Available Darren D Lynn,1 Tamara Umari,1 Cory A Dunnick,2,3 Robert P Dellavalle2–4 1Department of Dermatology, University of Colorado School of Medicine, 2Department of Dermatology, University of Colorado Anschutz Medical Campus, Aurora, 3Dermatology Service, US Department of Veterans Affairs, Eastern Colorado Health Care System, Denver, 4Department of Epidemiology, Colorado School of Public Health, University of Colorado Anschutz Medical Campus, Aurora, CO, USA Importance: Acne vulgaris is the most common skin condition affecting late adolescents across the globe. Although prior studies have evaluated epidemiologic patterns of acne vulgaris in various ethnicities and regions, adequate understanding of the worldwide burden of the disease associated with patients in their late adolescence (15–19-year olds remains lacking. Objective: To assess the global burden of the disease associated with acne vulgaris for late adolescents (15–19-year olds and provide an overview of the epidemiology, pathophysiology, and treatment options for acne in this population. Design: Database summary study. Setting: Global Burden of Disease Study 2010 database. Participants: Global Burden of Disease regions comprised countries with prevalence of acne vulgaris between the ages of 15 and 19 years. Main outcomes and measures: Geographic region-level disability-adjusted life year rates (per 100,000 persons associated with acne vulgaris in years 1990 through 2010. Median percentage change in disability-adjusted life year rates was estimated for each region across the specified study period. Conclusion and relevance: Acne vulgaris-associated disease burden exhibits global distribution and has continued to grow in prevalence over time within this population. This continued growth suggests an unmet dermatologic need worldwide for this disorder and potential opportunities for improved access and delivery of dermatologic care. Our analysis of the literature reveals numerous

  17. Identification of Small RNAs in Desulfovibrio vulgaris Hildenborough

    Energy Technology Data Exchange (ETDEWEB)

    Burns, Andrew; Joachimiak, Marcin; Deutschbauer, Adam; Arkin, Adam; Bender, Kelly

    2010-05-17

    Desulfovibrio vulgaris is an anaerobic sulfate-reducing bacterium capable of facilitating the removal of toxic metals such as uranium from contaminated sites via reduction. As such, it is essential to understand the intricate regulatory cascades involved in how D. vulgaris and its relatives respond to stressors in such sites. One approach is the identification and analysis of small non-coding RNAs (sRNAs); molecules ranging in size from 20-200 nucleotides that predominantly affect gene regulation by binding to complementary mRNA in an anti-sense fashion and therefore provide an immediate regulatory response. To identify sRNAs in D. vulgaris, a bacterium that does not possess an annotated hfq gene, RNA was pooled from stationary and exponential phases, nitrate exposure, and biofilm conditions. The subsequent RNA was size fractionated, modified, and converted to cDNA for high throughput transcriptomic deep sequencing. A computational approach to identify sRNAs via the alignment of seven separate Desulfovibrio genomes was also performed. From the deep sequencing analysis, 2,296 reads between 20 and 250 nt were identified with expression above genome background. Analysis of those reads limited the number of candidates to ~;;87 intergenic, while ~;;140 appeared to be antisense to annotated open reading frames (ORFs). Further BLAST analysis of the intergenic candidates and other Desulfovibrio genomes indicated that eight candidates were likely portions of ORFs not previously annotated in the D. vulgaris genome. Comparison of the intergenic and antisense data sets to the bioinformatical predicted candidates, resulted in ~;;54 common candidates. Current approaches using Northern analysis and qRT-PCR are being used toverify expression of the candidates and to further develop the role these sRNAs play in D. vulgaris regulation.

  18. ICG laser therapy of acne vulgaris

    Science.gov (United States)

    Tuchin, Valery V.; Altshuler, Gregory B.; Genina, Elina A.; Bashkatov, Alexey N.; Simonenko, Georgy V.; Odoevskaya, Olga D.; Yaroslavsky, Ilya V.

    2004-07-01

    The near-infrared (NIR) laser radiation due to its high penetration depth is widely used in phototherapy. In application to skin appendages a high selectivity of laser treatment is needed to prevent light action on surrounding tissues. Indocyanine Green (ICG) dye may provide a high selectivity of treatment due to effective ICG uploading by a target and its narrow band of considerable absorption just at the wavelength of the NIR diode laser. The goal of this study is to demonstrate the efficacy of the NIR diode laser phototherapy in combination with topical application of ICG suggested for soft and thermal treatment of acne vulgaris. 28 volunteers with facile or back-located acne were enrolled. Skin sites of subjects were stained by ICG and irradiated by NIR laser-diode light (803 or 809 nm). Untreated, only stained and only light irradiated skin areas served as controls. For soft acne treatment, the low-intensity (803 nm, 10 - 50 mW/cm2, 5-10 min) or the medium-intensity (809 nm, 150 - 190 mW/cm2, 15 min) protocols were used. The single and multiple (up to 8-9) treatments were provided. The individual acne lesions were photothermally treated at 18 W/cm2 (803 nm, 0.5 sec) without skin surface cooling or at 200 W/cm2 (809 nm, 0.5 sec) with cooling. The results of the observations during 1-2 months after the completion of the treatment have shown that only in the case of the multiple-wise treatment a combined action of ICG and NIR irradiation reduces inflammation and improves skin state during a month without any side effects. At high power densities (up to 200 W/cm2) ICG stained acne inflammatory elements were destructed for light exposures of 0.5 sec. Based on the concept that hair follicle, especially sebaceous gland, can be intensively and selectively stained by ICG due to dye diffusion through pilosebaceous canal and its fast uptake by living microorganisms, by vital keratinocytes of epithelium of the canal and sebaceous duct, and by rapidly proliferating

  19. Pemphigus vulgaris autoantibody profiling by proteomic technique.

    Directory of Open Access Journals (Sweden)

    Mina Kalantari-Dehaghi

    Full Text Available Pemphigus vulgaris (PV is a mucocutaneous blistering disease characterized by IgG autoantibodies against the stratified squamous epithelium. Current understanding of PV pathophysiology does not explain the mechanism of acantholysis in patients lacking desmoglein antibodies, which justifies a search for novel targets of pemphigus autoimmunity. We tested 264 pemphigus and 138 normal control sera on the multiplexed protein array platform containing 701 human genes encompassing many known keratinocyte cell-surface molecules and members of protein families targeted by organ-non-specific PV antibodies. The top 10 antigens recognized by the majority of test patients' sera were proteins encoded by the DSC1, DSC3, ATP2C1, PKP3, CHRM3, COL21A1, ANXA8L1, CD88 and CHRNE genes. The most common combinations of target antigens included at least one of the adhesion molecules DSC1, DSC3 or PKP3 and/or the acetylcholine receptor CHRM3 or CHRNE with or without the MHC class II antigen DRA. To identify the PV antibodies most specific to the disease process, we sorted the data based on the ratio of patient to control frequencies of antigen recognition. The frequency of antigen recognition by patients that exceeded that of control by 10 and more times were the molecules encoded by the CD33, GP1BA, CHRND, SLC36A4, CD1B, CD32, CDH8, CDH9, PMP22 and HLA-E genes as well as mitochondrial proteins encoded by the NDUFS1, CYB5B, SOD2, PDHA1 and FH genes. The highest specificity to PV showed combinations of autoantibodies to the calcium pump encoded by ATP2C1 with C5a receptor plus DSC1 or DSC3 or HLA-DRA. The results identified new targets of pemphigus autoimmunity. Novel autoantibody signatures may help explain individual variations in disease severity and treatment response, and serve as sensitive and specific biomarkers for new diagnostic assays in PV patients.

  20. The optimal hyperspectral quantitative models for chlorophyll-a of chlorella vulgaris

    Science.gov (United States)

    Cheng, Qian; Wu, Xiuju

    2009-09-01

    Chlorophyll-a of Chlorella vulgaris had been related with spectrum. Based on hyperspectral measurement for Chlorella vulgaris, the hyperspectral characteristics of Chlorella vulgaris and their optimal hyperspectral quantitative models of chlorophyll-a (Chla) estimation were researched in situ experiment. The results showed that the optimal hyperspectral quantitative model of Chlorella vulgaris was Chla=180.5+1125787(R700)'+2.4 *109[(R700)']2 (P0Chlorella vulgaris, two reflectance crests were around 540 nm and 700 nm and their locations moved right while Chl-a concentration increased. The reflectance of Chlorella vulgaris decreases with Cha concentration increase in 540 nm, but on the contrary in 700nm.

  1. A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

    Institute of Scientific and Technical Information of China (English)

    Zhankui Liu; Shengshun Tan; Chunshui Yu; Jinghua Fan; Zhuanli Bai; Junjie Li

    2007-01-01

    Objective:To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods:Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons.Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results :Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients,while nine other different proteins expressing from healthy volunteers. Conclusion:The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.

  2. OXIDANT/ANTIOXIDANT STATUS IN OBESE ADOLESCENT FEMALES WITH ACNE VULGARIS

    OpenAIRE

    Abulnaja Khalid

    2009-01-01

    Background and Objectives: Acne vulgaris is a distressing skin condition, which can carry with it significant psychological disability. Oxidant/antioxidant imbalance leads to increased production of free radicals, that cause many diseases. Some nutrients, along with systemic oxidative stress, have been implicated in acne vulgaris. The goal of the present study was to assess oxidant and antioxidant status in correlation with the incidence of acne vulgaris in adolescent obese females. Materia...

  3. Mutualistic growth of the sulfate-reducer Desulfovibrio vulgaris Hildenborough with different carbohydrates.

    Science.gov (United States)

    Santana, M M; Portillo, M C; Gonzalez, J M

    2012-01-01

    Desulfovibrio vulgaris Hildenborough genome presents a phosphotransferase system putatively involved in the transport of carbohydrates. However, utilization of sugars by this sulfate-reducing bacterium has never been reported. Herein, we have observed proliferation of D. vulgaris Hildenborough with some carbohydrates, in mutualism with Stenotrophomonas maltophilia, a non-fermentative, gram-negative gammaproteobacterium, or Microbacterium, a gram-positive actinobacterium. These results suggest the importance of feedback interactions between different heterotrophic bacterial species including the alternative for D. vulgaris of exploiting additional organic resources and novel habitats. Thus, D. vulgaris strongly participates in the mineralization of carbohydrates both in complex natural and artificial systems.

  4. Interaction between Chlorella vulgaris and bacteria:interference and resource competition

    Institute of Scientific and Technical Information of China (English)

    QU Liang; WANG Renjun; ZHAO Peng; CHEN Ruinan; ZHOU Wenli; TANG Liuqing; TANG Xuexi

    2014-01-01

    Research of interaction mechanism between Chlorella vulgaris and two bacterial strains (Z-QD08 and Z-QS01) were conducted under laboratory conditions. Growth rates of bacteria and C. vulgaris were tested under co-culture conditions to evaluate the effects of concentrations of C. vulgaris and bacteria on their interactions. To test whether the availability of inorganic nutrients, vitamins and trace metals affects the interactions between C. vulgaris and bacteria, experiments were performed with or without the culture medium filtrate of C. vulgaris or bacteria. The results showed that the growth of C. vulgaris was promot-ed at low concentrations of bacteria (5×106 cells/ml), and expressed a positive correlation with the bacteria density, whereas opposite trend was observed for treatments with high bacteria density (10×106 cells/ml and 20×106 cells/ml). The growth rate of bacteria decreased with the increasing concentrations of C. vul-garis. The growth of bacteria Z-QD08 was inhibited by C. vulgaris through interference competition, while the mechanism for interaction between bacteria Z-QS01 and C. vulgaris was resource competition. The influence of cell density on the interaction between microalgae and bacteria was also discussed. These ex-periments confirm some elements of published theory on interactions between heterotrophic bacteria and microalgae and suggest that heterotrophic bacteria play an important role in the development of blooms in natural waters.

  5. Mucosal-dominant pemphigus vulgaris in a captopril-taking woman with angioedema.

    Science.gov (United States)

    Gornowicz-Porowska, Justyna; Dmochowski, Marian; Pietkiewicz, Pawel; Bowszyc-Dmochowska, Monika

    2015-01-01

    We describe a 39-year-old woman with an apparent captopril-induced, contact mucosal-dominant pemphigus vulgaris and angioedema, who took captopril during a bout of arterial hypertension. This exposure suggests that captopril and pathophysiology of angioedema stimulated the development of pemphigus vulgaris, which was diagnosed using the novel, indirect immunofluorescence BIOCHIP mosaic, with the modification to detect serum IgG4 autoantibodies. We discuss the patient, who experienced a chain of events leading to the active stage of pemphigus vulgaris, and review concepts of pemphigus vulgaris inducible by drugs and pathological immunity.

  6. Thymus vulgaris essential oil: chemical composition and antimicrobial activity.

    Science.gov (United States)

    Borugă, O; Jianu, C; Mişcă, C; Goleţ, I; Gruia, A T; Horhat, F G

    2014-01-01

    The study was designed to determine the chemical composition and antimicrobial properties of the essential oil of Thymus vulgaris cultivated in Romania. The essential oil was isolated in a yield of 1.25% by steam distillation from the aerial part of the plant and subsequently analyzed by GC-MS. The major components were p-cymene (8.41%), γ-terpinene (30.90%) and thymol (47.59%). Its antimicrobial activity was evaluated on 7 common food-related bacteria and fungus by using the disk diffusion method. The results demonstrate that the Thymus vulgaris essential oil tested possesses strong antimicrobial properties, and may in the future represent a new source of natural antiseptics with applications in the pharmaceutical and food industry.

  7. Case of lupus vulgaris diagnosed 50 years after onset.

    Science.gov (United States)

    Uttawichai, Pattanawadee; Igarashi, Tsukasa; Kawana, Seiji

    2009-02-01

    Cutaneous tuberculosis is an infrequent form of extrapulmonary tuberculosis, but is a symptom that can lead to diagnosis of tuberculosis. We describe a case of lupus vulgaris in a 79-year-old woman who had a 50-year history of a slowly growing plaque on her right cheek. She visited many hospitals without resolution and the plaque gradually enlarged. Recently, she was misdiagnosed with eczema and prescribed topical steroids that had no effect, and she subsequently visited our outpatient clinic. A diagnosis of lupus vulgaris was made based on histopathology, culture and polymerase chain reaction, and isoniazid, rifampicin and ethambutol were administered as antituberculosis treatment. Although the incidence of cutaneous tuberculosis has decreased significantly in developed countries, knowledge and awareness of the disease are still of importance for proper diagnosis and treatment.

  8. Biodiesel production in crude oil contaminated environment using Chlorella vulgaris.

    Science.gov (United States)

    Xaaldi Kalhor, Aadel; Mohammadi Nassab, Adel Dabbagh; Abedi, Ehsan; Bahrami, Ahmad; Movafeghi, Ali

    2016-12-01

    Biodiesel is a valuable alternative to fossil fuels and many countries choose biodiesel as an unconventional energy source. A large number of investigations have been done on microalgae as a source of oil production. In recent years, wastewater pollutions have caused many ecological problems, and therefore, wastewater phycoremediation has attracted the international attention. This paper studied the cultivation of Chlorella vulgaris in a crude oil polluted environment for biodiesel production. Intended concentrations were 10 and 20gperliter (crude oil/water) at two times. The results showed that the growth of C. vulgaris was improved in wastewater and the maximum amount of dry mass and oil was produced at the highest concentration of crude oil (0.41g and 0.15g/l, respectively). In addition, dry mass and oil yield of the microalga were significantly enhanced by increasing the experiment duration.

  9. EFFECT OF CAPSAICIN ON SUBSTANCE P IN PSORIASIS VULGARIS

    Institute of Scientific and Technical Information of China (English)

    王万卷; 谭升顺

    2003-01-01

    Objective To the investigate the mechanism of capsaicin in psoriasis vulgaris. Methods Substance P(SP) in psoriatic lesions before and 6 weeks after the treatment with capsaicin was detedted by radioimmunoassary. Results After 3 weeks and 6 weeks treatment with capsaicin, SP in psoriatic lesions was decreased (P<0.05), while it in the self-control group was not decreased; Overall the efficient incidence in therapeutic group was 78.8% , while it in the control group was 36.8%. There was significant difference between them (χ2=16.30, P<0.001). Conclusion Capsaicin inhibits dermal inflammatory responses and proliferation of keratinocytes by decreasing the expression of SP in psoriasis vulgaris.

  10. Ulcerative lupus vulgaris of face: an uncommon presentation in India.

    Science.gov (United States)

    Padmavathy, L; Rao, L Lakshmana; Ethirajan, N; Krishnaswami, B

    2007-01-01

    Tuberculosis affects the population world wide, more among those living in developing countries. The incidence of tuberculosis registered an upward trend even in developed countries, with the advent of HIV infection. Cutaneous tuberculosis accounts for about 1% of cases of extra-pulmonary tuberculosis. Cutaneous tuberculosis presents with various lesions ranging from ulcerative to proliferative or hyperkeratotic lesions. The lesions may sometimes be associated with marked destruction of the tissues resulting in marked disfigurement, especially when it involves face as seen in cases of Lupus Vulgaris. A case of Lupus Vulgaris in a young woman with extensive ulceration of face which responded to ATT resulting in scarring of the face is reported for its rarity amongst Indian population as against western population.

  11. Annular lupus vulgaris: an unusual case undiagnosed for five years.

    Science.gov (United States)

    Gönül, Müzeyyen; Kiliç, Arzu; Külcü Cakmak, Seray; Gül, Ulker; Koçak, Oğuzhan; Demiriz, Murat

    2007-01-01

    Tuberculosis is still a serious problem in both developing and developed countries. It is often confused with various cutaneous disorders both clinically and histopathologically.A 46-year-old woman attended our clinic with progressive, asymptomatic, annular skin lesions on her right upper extremity for 5 years. She had received many different therapies for these lesions at other institutions previously but these medications were not effective and the lesions deteriorated. On dermatological examination, well-demarcated, irregular bordered, violaceous colored, elevated and crusted annular lesions on her right hand dorsum and forearm were observed. She was diagnosed as having lupus vulgaris clinically and histopathologically. Antituberculosis therapy was administered and regression of the lesions started in the second week of medication.We report a case of long-standing, undiagnosed and uncommon, annular form of lupus vulgaris. We want to stress that clinical and histopathological findings are still important for the diagnosis of cutaneous tuberculosis.

  12. Phaseolus vulgaris RbohB functions in lateral root development

    Science.gov (United States)

    Montiel, Jesús; Arthikala, Manoj-Kumar; Quinto, Carmen

    2013-01-01

    Respiratory burst oxidase homologs (RBOHs) catalyze the reduction of oxygen to generate superoxide anion, a kind of reactive oxygen species (ROS). The ROS produced by RBOHs play essential roles in diverse processes, such as root hair development, stomata closure and signaling mechanisms in response to abiotic stimuli and during plant-pathogen interactions. Recently, we found that PvRbohB silencing in transgenic Phaseolus vulgaris roots had a negative impact on lateral root density. In this work, we show that the downregulation of PvRbohB affects both the growth and ROS levels in recently emerged lateral roots. In addition, we found that the PvRbohB promoter was activated during lateral root primordium initiation in the pericycle, and remained active throughout lateral root development. This study identifies RBOHs as potentially important players in lateral root development in P. vulgaris. PMID:23221754

  13. Respose of Phaseolus vulgaris L. cultivars to drought stress

    Directory of Open Access Journals (Sweden)

    Amalia Domínguez

    2014-01-01

    Full Text Available Tolerance to drought is a desired cultivars of Phaseolus vulgaris L. The present study aimed to determine the response of 22 cultivars of common bean during the early stages of vegetative development character. The plants were subjected to irrigation at 70% or 20% of field capacity (FC for seven days and the indicators were measured relative water content, stomatal opening, stomatal index, proline content and total phenols in leaves. The data obtained were processed using a principal component analysis and the variables studied were represented by a bivariate graph (biplot. It was possible to group the cultivars based on their response in tolerant, moderately tolerant and susceptible to water stress condition induced by irrigation at 20% FC. Stomatal opening and relative water content were recommended to be used as criteria for selecting cultivars tolerant to water stress indicators bean. Key words: PCA, Phaseolus vulgaris L., proline, water stress

  14. Significance of diet in treated and untreated acne vulgaris

    Directory of Open Access Journals (Sweden)

    Alicja Kucharska

    2016-05-01

    Full Text Available The relationship between diet and acne is highly controversial. Several studies during the last decade have led dermatologists to reflect on a potential link between diet and acne. This article presents the latest findings on a potential impact that diet can have on pathogenesis of acne vulgaris. The association between diet and acne can no longer be dismissed. Compelling evidence shows that high glycemic load diets may exacerbate acne. Dairy ingestion appears to be weakly associated with acne and the roles of omega-3 fatty acids, dietary fiber, antioxidants, vitamin A, zinc and iodine remain to be elucidated. The question of what the impact of diet is on the course of acne vulgaris still remains unclear.

  15. Significance of diet in treated and untreated acne vulgaris.

    Science.gov (United States)

    Kucharska, Alicja; Szmurło, Agnieszka; Sińska, Beata

    2016-04-01

    The relationship between diet and acne is highly controversial. Several studies during the last decade have led dermatologists to reflect on a potential link between diet and acne. This article presents the latest findings on a potential impact that diet can have on pathogenesis of acne vulgaris. The association between diet and acne can no longer be dismissed. Compelling evidence shows that high glycemic load diets may exacerbate acne. Dairy ingestion appears to be weakly associated with acne and the roles of omega-3 fatty acids, dietary fiber, antioxidants, vitamin A, zinc and iodine remain to be elucidated. The question of what the impact of diet is on the course of acne vulgaris still remains unclear.

  16. [Clinical features and genetics of the ichthyosis vulgaris group].

    Science.gov (United States)

    Traupe, H; Happle, R

    1980-12-11

    Combined application of clinical, genetic and histological criteria in general allows a definite diagnosis of autosomal dominant ichthyosis vulgaris and of X-linked recessive ichthyosis. For differential diagnosis, the following rare syndromes should be considered: ichthyosis bullosa: Refsum syndrome; Jung-Vogel syndrome; ichthyosis with corneal opacity, pili torti and alopecia; ichthyosis with deafness, pili torti and dental anomalies; and ichthyosis with hepatosplenomegaly and cerebellar degeneration.

  17. The morphology and adhesion mechanism of Octopus vulgaris suckers.

    Science.gov (United States)

    Tramacere, Francesca; Beccai, Lucia; Kuba, Michael; Gozzi, Alessandro; Bifone, Angelo; Mazzolai, Barbara

    2013-01-01

    The octopus sucker represents a fascinating natural system performing adhesion on different terrains and substrates. Octopuses use suckers to anchor the body to the substrate or to grasp, investigate and manipulate objects, just to mention a few of their functions. Our study focuses on the morphology and adhesion mechanism of suckers in Octopus vulgaris. We use three different techniques (MRI, ultrasonography, and histology) and a 3D reconstruction approach to contribute knowledge on both morphology and functionality of the sucker structure in O. vulgaris. The results of our investigation are two-fold. First, we observe some morphological differences with respect to the octopus species previously studied (i.e., Octopus joubini, Octopus maya, Octopus bimaculoides/bimaculatus and Eledone cirrosa). In particular, in O. vulgaris the acetabular chamber, that is a hollow spherical cavity in other octopuses, shows an ellipsoidal cavity which roof has an important protuberance with surface roughness. Second, based on our findings, we propose a hypothesis on the sucker adhesion mechanism in O. vulgaris. We hypothesize that the process of continuous adhesion is achieved by sealing the orifice between acetabulum and infundibulum portions via the acetabular protuberance. We suggest this to take place while the infundibular part achieves a completely flat shape; and, by sustaining adhesion through preservation of sucker configuration. In vivo ultrasonographic recordings support our proposed adhesion model by showing the sucker in action. Such an underlying physical mechanism offers innovative potential cues for developing bioinspired artificial adhesion systems. Furthermore, we think that it could possibly represent a useful approach in order to investigate any potential difference in the ecology and in the performance of adhesion by different species.

  18. Acne Vulgaris and Acne Rosacea: An Update in Etiopathogenesis

    OpenAIRE

    Ozlem Ekiz

    2014-01-01

    Acne vulgaris is a chronic inflammatory disease of the pilosebaceous unit, characterized by comedones, papules, pustules, nodules, and scars rarely. The major pathogenic factors are abnormal follicular differentiation and increased ductal cornification, abnormal activity of sebaceous glands, microbial colonization of pilosebaceous units by Propionibacterium acnes and inflammation. Rosacea is a common, chronic inflammatory relapsing skin disorder of the central area of the face characterized b...

  19. Human platelet aggregation inhibitors from thyme (Thymus vulgaris L.).

    Science.gov (United States)

    Okazaki, Kenji; Kawazoe, Kazuyoshi; Takaishi, Yoshihisa

    2002-06-01

    Two antiaggregant compounds, thymol (compound 1) and 3,4,3',4'-tetrahydroxy-5,5'-diisopropyl-2,2'-dimethylbiphenyl (compound 2) were isolated from the leaves of thyme (Thymus vulgaris L.). The structures were determined by (1)H-, (13)C-NMR and mass spectra (MS) studies. These compounds inhibited platelet aggregation induced by collagen, ADP, arachidonic acid (AA) and thrombin except that compound 2 did not inhibit platelet aggregation induced by thrombin.

  20. Standardization of phaseolus vulgaris valves by macro- and microscopic characteristics

    Directory of Open Access Journals (Sweden)

    Анна Ігорівна Крюкова

    2016-03-01

    Full Text Available During the last years on the Ukrainian pharmaceutical market a tendency to increase the range of the national herbal remedies is observed. Native flora herbs, having a sufficient resource base, deserve special attention and are characterized by a number of economic benefits for manufacturers. The implementation of herbal remedies in medical practice is accompanied by the necessity to improve the quality control of herbal material and to develop normative documents. Macro- and microscopy analysis becomes important at standardization of herbal material especially cut and powdered.Aim. The aim of our work was to carry out macro- and microscopy research of Phaseolus vulgaris and to determine its diagnostic morphologic and anatomic features.Methods. The conventional research methods in pharmacognosy have been used; photomicrography has been taken by using microscope MC-10 and Samsung PL 50 camera.Results. As a result of research, the microscopic diagnostic characteristics for Phaseolus vulgaris valves have been proposed, e.g.: the valves are elongated, straight, navicular or grooved, twisted to some extent; the external surface is smooth or slightly undulating, mat, pale yellow or yellow; the internal surface is shiny, white or yellowish-white. Sometimes carpophore (stalk may occur. The microscopic diagnostic features have been determined and offered, e.g.: cells of outer epidermis are pentagonal and hexagonal, with plicated cuticle; stomata are surrounded by 3–4 subsidiary narrow cells, additionally surrounded by 5–6 narrow cells; cover trichomes and glandular trichomes are elongated, pointed and unbranched; xylem vessels and tracheids; non-bundled structure stalk fragments with pericyclic sclerenchyma and secretory cells in the phloem.Conclusion. Research on determination of the macro- and microscopic characteristics of Phaseolus vulgaris valves has been carried out. The diagnostic morphological and anatomic elements in herbal material have

  1. An unusual central retinal dystrophy associated with ichthyosis vulgaris.

    Science.gov (United States)

    Saatci, O A; Ozbek, Z; Köse, S; Durak, I; Kavukçu, S

    2000-06-01

    A number of ichthyosis syndromes may have retinal abnormalities such as the retinitis pigmentosa-like diffuse rod-cone dystrophy in Refsum's syndrome and the maculopathy in Sjögren-Larsson syndrome. We present two sisters who have an unusual, almost identical, bilaterally symmetric central retinal dystrophy associated with ichthyosis vulgaris in the absence of other systemic disorders. We believe that this dystrophy has not been previously described in patients with any of the known varieties of ichthyosis.

  2. Lupus vulgaris of the popliteal fossa: a delayed diagnosis.

    Science.gov (United States)

    Altunay, Ilknur Kivanc; Kayaoglu, Semra; Ekmekci, Tugba Rezan; Kutlu, Safiye; Arpag, Esra Saygin

    2007-07-13

    Lupus vulgaris (LV) is the most common form of cutaneous tuberculosis. It commonly presents on the head and neck regions. The diagnosis may be difficult when LV occurs at unexpected regions or in unusual clinical forms. Sometimes special stains for the organism and mycobacterial cultures may be negative. Nevertheless, it is usually possible to reach the correct diagnosis of LV using clinical and histopathological findings. But at times, a therapeutic trial with antitubercular agents may be required.

  3. Anti-androgens in treatment of acne vulgaris.

    Science.gov (United States)

    Amer, M; Ramadan, A; Abdel Monem, A

    1985-10-01

    Seventy-five women suffering from acne vulgaris classified into three groups according to the grade of the disease. Anti-androgenic tablets cyproterone acetate (CPA), were given in three cycles. The total number of patients whose condition improved was 52% after the first cycle, 55% after the second cycle, and 81.3% after the third cycle. No serious side effects were encountered. CPA is a suitable therapeutic modility for AV in women who use contraceptives.

  4. Distribution and Development of the TCM Syndromes in Psoriasis Vulgaris

    Institute of Scientific and Technical Information of China (English)

    ZHANG Guang-zhong; WANG Ju-sheng; WANG Ping; JIANG Chun-yan; DENG Bing-xu; LI Ping; ZHAO Yi-ming; LIU Wa-li; Qu Xing; CHEN Wei-wen; ZENG Lin; ZHOU Dong-mei; SUN Li-yun; LI Ruo-yu

    2009-01-01

    Objective:To study the distribution and development rules of the TCM syndromes in psoriasis vulgaris.Methods:Based on the clinical epidemiologic mass survey, the study was carried out by means of a status survey, multi-center and large-sample research.The data base was set up by EPIINFO6.0.The SPSS was used to do the statistical analyses in 2651 cases of psoriasis vulgaris to study the correlations among the distribution and development of the TCM syndromes, the stages of the disease, nationality, psoriasis history,family history, smoking history, alcohol drinking history, and severity of the disease.Results:The TCM syndromes in psoriasis vulgaris mainly include the blood-heat syndrome (53.8%), blood-dryness syndrome (27.4%), and blood-stasis syndrome (18.1%).Other syndromes were rarely seen, covering 0.6%.The concurrent syndromes mainly involve dampness, heat, blood stasis and toxin.The distribution differences of the main syndromes at different stages of the disease had statistical significance (P<0.01).The syndrome distribution is not related with nationality and family history (P>0.05), but it was closely related with the psoriasis history, smoking history, alcohol drinking history, and severity of the disease (P<0.01).Conclusion:At the initial stage, psoriasis vulgaris usually manifests itself as the blood-heat syndrome, and later it may be improved or turn into the blood-dryness or blood-stasis syndrome.Smoking, alcohol consumption, and severity of the disease may play a role in the syndrome's transformation.

  5. The morphology and adhesion mechanism of Octopus vulgaris suckers.

    Directory of Open Access Journals (Sweden)

    Francesca Tramacere

    Full Text Available The octopus sucker represents a fascinating natural system performing adhesion on different terrains and substrates. Octopuses use suckers to anchor the body to the substrate or to grasp, investigate and manipulate objects, just to mention a few of their functions. Our study focuses on the morphology and adhesion mechanism of suckers in Octopus vulgaris. We use three different techniques (MRI, ultrasonography, and histology and a 3D reconstruction approach to contribute knowledge on both morphology and functionality of the sucker structure in O. vulgaris. The results of our investigation are two-fold. First, we observe some morphological differences with respect to the octopus species previously studied (i.e., Octopus joubini, Octopus maya, Octopus bimaculoides/bimaculatus and Eledone cirrosa. In particular, in O. vulgaris the acetabular chamber, that is a hollow spherical cavity in other octopuses, shows an ellipsoidal cavity which roof has an important protuberance with surface roughness. Second, based on our findings, we propose a hypothesis on the sucker adhesion mechanism in O. vulgaris. We hypothesize that the process of continuous adhesion is achieved by sealing the orifice between acetabulum and infundibulum portions via the acetabular protuberance. We suggest this to take place while the infundibular part achieves a completely flat shape; and, by sustaining adhesion through preservation of sucker configuration. In vivo ultrasonographic recordings support our proposed adhesion model by showing the sucker in action. Such an underlying physical mechanism offers innovative potential cues for developing bioinspired artificial adhesion systems. Furthermore, we think that it could possibly represent a useful approach in order to investigate any potential difference in the ecology and in the performance of adhesion by different species.

  6. [Correction of experimental anxiety behavior by meadowsweet (Filipendula vulgaris) extracts].

    Science.gov (United States)

    Vengerovskiĭ, A I; Suslov, N I; Kaĭgorodtsev, A V

    2011-01-01

    Influence of aqueous and aqueous-ethanol extracts of meadowsweet (Filipendula vulgaris) on the behavior of albino mice in anxiety models has been studied. It is established that the aqueous and 95% aqueous ethanol extracts of meadowsweet produce the most expressed anxiolytic action in the elevated plus-maze, open field, and emotional reaction tests. The anxiolytic activity of these extracts exceeds that of the reference valerian extract.

  7. Nail changes in female pemphigus vulgaris patients on immunosuppressive therapy

    OpenAIRE

    M.M. El-Komy, MD; D.M. Abdel Halim, MD; N. Samir, MD; R.A. Hegazy, MD; H.I. Gawdat, MD; S.A. Shoeb, MD

    2015-01-01

    Background: Pemphigus vulgaris (PV) patients receiving immunosuppressive therapy may develop nail alterations resulting from infection, skin disorder, or drug regimen. Objective: This study aims to describe nail changes in PV female patients receiving immunosuppressive therapy and to report the frequency of associated fungal and bacterial growth in the patients’ nails. Methods: Twenty-five female PV patients who had at least one acquired finger or toenail abnormality and had been admini...

  8. Isolation and Expression Analysis of GST Gene Encoding Glutathione S-transferase from Senecio cruentus%瓜叶菊谷胱甘肽转移酶基因GST的分离及表达分析

    Institute of Scientific and Technical Information of China (English)

    金雪花; 洪艳; 黄河; 戴思兰; 朱嫄

    2013-01-01

    从瓜叶菊中克隆了10个谷胱甘肽转移酶(GST)基因,命名为ScGST1~10.序列相似性比较与基因表达分析结果显示,ScGST3可能是一个花青素苷转运相关的候选基因.对RT-PCR扩增的ScGST3序列分析发现,其包含一个639 bp的开放阅读框,编码212个氨基酸残基,含有3个外显子和2个内含子,属于phi型GST;氨基酸序列比较分析表明,其与仙客来(Cyclamen persicum)CkmGST3、香石竹(Dianthus caryophyllus) DcGSTF2和矮牵牛(Petunia hybrida)PhAN9等花青素苷转运相关的GST具有较高的相似性;荧光定量PCR分析表明,该基因在含有花青素苷的组织中表达信号较强,在花序发育早期表达丰度最高,花序开放末期表达量下降.据此推测分离得到的ScGST3可能与瓜叶菊中花青素苷的转运与积累相关.%We cloned 10 glutathione transferase (GST) genes from Senecio cruentus and called after ScGST1-10.ScGST3 was considered to be an alternative gene which related to the transfer of anthocyanin based on the similarity compare of sequence and analysis of gene expression.The amplified sequence of ScGST3 contains a 639 bp open reading frame,which encodes 212 amino acid residues.It also contains 3 exons and 2 introns and belongs to phi type GST.The compare analysis of amino acid sequence showed that the similarity between ScGST3 and other GSTs that related to the transfer of anthocyanin (such as DcGSTF2 in Dianthus caryophyllus,CkmGST3 in Cyclamen persicum and PhAN9 in Petunia hybrida and so on) were higher.The analysis of fluorescent quantitation PCR showed that this gene had a higher expression in the tissues which contain anthocyanin.The expression reached the highest abundance and decreased at the early and final stages of inflorescence development,respectively.In conclusion,we inferred that ScGST3 might associate with the transfer and accumulation of anthocyanin in Senecio cruentus.

  9. Vitreoscilla hemoglobin gene ( vgb) improves lutein production in Chlorella vulgaris

    Science.gov (United States)

    Ma, Ruijuan; Lin, Xiangzhi

    2014-03-01

    Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the efficiency of cell respiration and metabolism. In this study, we introduced a Vitreoscilla hemoglobin gene ( vgb) into Chlorella vulgaris by Agrobacterium tumefaciens -mediated transformation (ATMT). PCR analysis confirmed that the vgb gene was successfully integrated into the Chlorella vulgaris genome. Analysis of biomass obtained in shake flasks revealed transformant biomass concentrations as high as 3.28 g/L, which was 38.81% higher than that of the wild-type strain. Lutein content of transformants also increased slightly. Further experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants, which was 36.77% higher than that of the wild-type strain. The above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris, with applications to lutein production from Chlorella during fermentation.

  10. Photon up-conversion increases biomass yield in Chlorella vulgaris.

    Science.gov (United States)

    Menon, Kavya R; Jose, Steffi; Suraishkumar, Gadi K

    2014-12-01

    Photon up-conversion, a process whereby lower energy radiations are converted to higher energy levels via the use of appropriate phosphor systems, was employed as a novel strategy for improving microalgal growth and lipid productivity. Photon up-conversion enables the utilization of regions of the solar spectrum, beyond the typical photosynthetically active radiation, that are usually wasted or are damaging to the algae. The effects of up-conversion of red light by two distinct sets of up-conversion phosphors were studied in the model microalgae Chlorella vulgaris. Up-conversion by set 1 phosphors led to a 2.85 fold increase in biomass concentration and a 3.2 fold increase in specific growth rate of the microalgae. While up-conversion by set 2 phosphors resulted in a 30% increase in biomass and 12% increase in specific intracellular neutral lipid, while the specific growth rates were comparable to that of the control. Furthermore, up-conversion resulted in higher levels of specific intracellular reactive oxygen species in C. vulgaris. Up-conversion of red light (654 nm) was shown to improve biomass yields in C. vulgaris. In principle, up-conversion can be used to increase the utilization range of the electromagnetic spectrum for improved cultivation of photosynthetic systems such as plants, algae, and microalgae.

  11. Photosynthetic and cellular toxicity of cadmium in Chlorella vulgaris.

    Science.gov (United States)

    Ou-Yang, Hui-Ling; Kong, Xiang-Zhen; Lavoie, Michel; He, Wei; Qin, Ning; He, Qi-Shuang; Yang, Bin; Wang, Rong; Xu, Fu-Liu

    2013-12-01

    The toxic effects of cadmium (Cd) on the green alga Chlorella vulgaris were investigated by following the response to Cd of various toxicity endpoints (cell growth, cell size, photochemical efficiency of PSII in the light or Φ(PSII), maximal photochemical efficiency or Fv/Fm, chlorophyll a fluorescence, esterase activity, and cell viability). These toxicity endpoints were studied in laboratory batch cultures of C. vulgaris over a long-term 96-h exposure to different Cd concentrations using flow cytometry and pulse amplitude modulated fluorometry. The sequence of sensitivity of these toxicity endpoints was: cell yield > Φ(PSII) ≈ esterase activity > Fv/Fm > chlorophyll a fluorescence ≈ cell viability. It is shown that cell apoptosis or cell death only accounted for a minor part of the reduction in cell yield even at very high algistatic free Cd²⁺ concentrations, and other mechanisms such as blocked cell divisions are major contributors to cell yield inhibition. Furthermore, cadmium may affect both the electron donors and acceptors of the electron transport chain at high free Cd²⁺ concentration. Finally, the resistance of cells to cell death was size-dependent; medium-sized cells had the highest toxicity threshold. The present study brings new insights into the toxicity mechanisms of Cd in C. vulgaris and provides a detailed comparison of the sensitivity of various Cd toxicity endpoints.

  12. Ammonium reduces chromium toxicity in the freshwater alga Chlorella vulgaris.

    Science.gov (United States)

    Liu, Jingqing; Sun, Zhengqi; Lavoie, Michel; Fan, Xiaoji; Bai, Xiaocui; Qian, Haifeng

    2015-04-01

    The aim of the present study was to investigate the protective effect of ammonium (NH4 (+)) on Cr toxicity to the freshwater alga Chlorella vulgaris. We followed an array of cellular functions and biomolecules in C. vulgaris cells exposed to 50 or 100 μM Cr at three different initial NH4 (+) concentrations (0.5, 3, and 10 mM). The results showed that Cr strongly inhibited cell yield of C. vulgaris, but 10 mM NH4 (+) could decrease by more than two-fold Cr toxicity on cell yield compared to exposure to 0.5 mM NH4 (+). Cr toxicity on gene transcripts and cellular substructure was also much lower at high than at low NH4 (+). Our results suggest that this protecting effect of NH4 (+) on intracellular Cr toxicity could be due to several factors, such as enhance uptake of phosphorus, increase in C and N assimilation efficiency, and increase transcription of photosynthesis-related genes.

  13. Growth of Chlorella vulgaris and associated bacteria in photobioreactors.

    Science.gov (United States)

    Lakaniemi, Aino-Maija; Intihar, Veera M; Tuovinen, Olli H; Puhakka, Jaakko A

    2012-01-01

    The aim of this study was to test three flat plate photobioreactor configurations for growth of Chlorella vulgaris under non-axenic conditions and to characterize and quantify associated bacterial communities. The photobioreactor cultivations were conducted using tap water-based media to introduce background bacterial population. Growth of algae was monitored over time with three independent methods. Additionally, the quantity and quality of eukaryotes and bacteria were analysed using culture-independent molecular tools based on denaturing gradient gel electrophoresis (PCR-DGGE) and quantitative polymerase chain reaction (QPCR). Static mixers used in the flat plate photobioreactors did not generally enhance the growth at the low light intensities used. The maximum biomass concentration and maximum specific growth rate were 1.0 g l(-1) and 2.0 day(-1) respectively. Bacterial growth as determined by QPCR was associated with the growth of C. vulgaris. Based on PCR-DGGE, bacteria in the cultures mainly originated from the tap water. Bacterial community profiles were diverse but reproducible in all flat plate cultures. Most prominent bacteria in the C. vulgaris cultures belonged to the class Alphaproteobacteria and especially to the genus Sphingomonas. Analysis of the diversity of non-photosynthetic microorganisms in algal mass cultures can provide useful information on the public health aspects and unravel community interactions.

  14. Metastasized squamous cell carcinoma developed on lupus vulgaris.

    Science.gov (United States)

    Pătraşcu, V; Georgescu, Claudia Valentina; Tănase, Loredana Elena; Mogoantă, S S

    2008-01-01

    Lupus vulgaris (LV) is the most frequent cutaneous tuberculosis, representing more than 55% of the tuberculoses with this location. Malignization can occur after a long latency (10-30 years), in 1-2% of the cases, and it is mainly in squamous cell carcinoma. The histological exam is highly important in the observation of neoplasic transformations. The authors present a 59-years-old female patient, from the rural environment, working as a farmer, with lupus vulgaris developing since her first childhood years. It started at the age of 2 years, at the right ear lobule, after the empiric perforation for earrings. The evolution was progressive, eccentric, interesting the pinna and the right cheek in the meanwhile. At the first examination, in 2002, a diffuse mass of red-yellowish infiltration was found at the level of the right ear and the right cheek. In the following two years, an ulcero-vegetating tumor developed at the level of the right ear lobule, accompanied by the presence of a right retromandibular adenopathy, of about 1 cm, which was proved by the histopathologic exam to be a squamous cell carcinoma developed from a lupus vulgaris. After scraping out the right retromandibular ganglion, detected by palpation, a histological exam showed ganglion metastasis.

  15. Detection of apoptosis in pemphigus vulgaris by TUNEL technique*

    Science.gov (United States)

    Cuevas-Gonzalez, Juan Carlos; Vega-Memíje, Maria Elisa; García-Vázquez, Francisco Javier; Aguilar-Urbano, Marco António

    2016-01-01

    Background Pemphigus is part of a group of blistering diseases that affect the skin and mucous membranes. Based on its autoimmune origin, autoantibodies develop in pemphigus that are directed toward cell surface components of keratinocytes. However, some data cannot be explained, such as the lack of a relationship between autoantibody levels and the severity of clinical manifestations, treatment resistance, the presence of inflammatory infiltrates and the potential occurrence of apoptosis as determinants of vesicle formation. Objective To examine the presence of apoptosis in pemphigus vulgaris by TUNEL technique. Methods In this cross-sectional study, we selected 15 paraffin-embedded tissues from subjects who were diagnosed with pemphigus vulgaris by hematoxylin and eosin staining. The samples were subjected to TUNEL assay and examined under an Olympus BX61 fluorescence microscope. Positivity was categorized dichotomously, and the statistical analysis was performed using the X2 test. Results Positivity was observed in basal layer cells in 14 (93.3%) cases. In 13 (86.7%) of the positive cases, we noted espinosum and granular layers that formed the blister roof, and in 12 cases (80%), positive acantholytic cells were observed. Conclusions TUNEL positivity was observed in pemphigus vulgaris, implicating apoptosis in the pathophysiology of this condition, which can help guide the development of apoptotic blockers as therapeutics. PMID:27438195

  16. Profile of acne vulgaris-A hospital-based study from South India

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    Adityan Balaji

    2009-01-01

    Full Text Available Background: Acne vulgaris is believed to be the most common disease of the skin. There is no Indian study on the profile of acne vulgaris, markers of severe forms of acne vulgaris and a possible correlation between acne vulgaris and markers of androgenicity in females. Aim: To study the profile of acne vulgaris, its seasonal variation, relationship with smoking and possible correlation between acne vulgaris and markers of androgenicity in females. Methods: The study was conducted between August 2006 and June 2008. All patients with acne vulgaris who consented to participate in the study were included. The parameters evaluated included age, gender, age of onset, duration of lesions, site of lesions, grade, relation with menstrual cycle, markers of androgenicity, number of acne lesions such as comedones, papules pustules and nodules, number and site of post-acne scarring, post-acne hyperpigmentation, seasonal variation and history of smoking. Results: A total of 309 patients with acne vulgaris were included in the study. The frequency of acne vulgaris in our study was 1.068%. Mean age of the study group was 19.78 years. Male to female ratio was 1.25:1. The most common age group involved was 16 to 20 years (59.8%. Mean age of onset was 15.97 years. Face was involved in all the patients, followed by back (28.2%, chest (20.1%, neck (9.4% and arms (10%. In the older age groups, women were more likely to report having acne vulgaris than men ( P = 0.01. The closed comedones outnumbered open comedones by a factor of 4.9:1. A total of 186 patients (60.2% had grade 1 acne vulgaris, 85 (27.5% had grade 2 acne, 8 (2.6% had grade 3 acne and 30 (9.7% had grade 4 acne vulgaris. There was a higher incidence of scarring (39.5% and post-acne hyperpigmentation (24.6% in our study. In female patients, 57.7% had premenstrual flare and 12.4% had cutaneous markers of androgenicity. There was no association between severity of acne vulgaris and other markers of

  17. Antibacterial activity of Thymus vulgaris essential oil alone and in combination with other essential oils

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    MAHENDRA RAI

    2012-07-01

    Full Text Available Kon K, Rai M. 2012. Antibacterial activity of Thymus vulgaris essential oil alone and in combination with other essential oils. Nusantara Bioscience 4: 50-56. Essential oils (EOs from plants represent an alternative approach in combating antibiotic-resistant bacteria. One of the EOs with proven antibacterial properties is Thymus vulgaris EO. The purpose of the present work was to investigate in vitro antibacterial activity of T. vulgaris EO alone and in combination with other EOs. The activity of T. vulgaris EO was screened in combination with 34 EOs against Staphylococcus aureus and Escherichia coli by disk diffusion method; then the most effective combinations were evaluated by broth microdilution method. Against S. aureus the synergistic effect was found in combination of T. vulgaris and Cinnamomum zeylonicum EOs with fractional inhibitory concentration (FIC index of 0.26; Juniperus communis and Picea abies EOs showed additive effect (FIC indexes were 0.74 and 0.78, respectively. Combination of T. vulgaris EO with Aniba rosaeodora and Melissa officinalis EOs demonstrated synergistic effect against E. coli (FIC indexes were 0.23 and 0.34, respectively; combination of T. vulgaris and Mentha piperita EOs was additive (FIC index 0.55. Therefore, combining T. vulgaris EO with other EOs has potential in further enhancing its antibacterial properties.

  18. High social phobia frequency and related disability in patients with acne vulgaris.

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    Bez, Yasin; Yesilova, Yavuz; Kaya, Mehmet Cemal; Sir, Aytekin

    2011-01-01

    Acne is an easily recognizable abnormality which may cause some adverse psychosocial consequences. We aimed to determine the social phobia frequency, social anxiety level, and disease related disability in a group of acne vulgaris patients. One-hundred and forty acne vulgaris patients and 98 healthy control subjects were included in the study. Acne severity was determined by the Global Acne Grading System (GAGS). A psychiatrist interviewed each participant and the Liebowitz Social Anxiety Scale (LSAS) was administered to all participants, who also completed the Hospital Anxiety and Depression Scale (HADS) and Sheehan Disability Scale (SDS). Social phobia was diagnosed in 45.7% of acne vulgaris patients and in 18.4% of control subjects. Acne vulgaris patients demonstrated higher performance avoidance and total avoidance scores in LSAS than controls. Acne vulgaris patients without social phobia had higher scores in HADS and LSAS than the acne vulgaris patients with social phobia. They were more disabled in their occupational, social, and familial lives. Social phobia diagnosis predicted disability at work, whereas education level predicted the disability in family life of acne vulgaris patients. Social phobia seems to be a common psychiatric comorbidity which may give rise to some additional disability among acne vulgaris patients.

  19. Insect resistance to sugar beet pests mediated by a Beta vulgaris proteinase inhibitor transgene

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    We transformed sugar beet (Beta vulgaris) hairy roots and Nicotiana benthamiana plants with a Beta vulgaris root gene (BvSTI) that codes for a serine proteinase inhibitor. BvSTI is a root gene cloned from the F1016 breeding line that has moderate levels of resistance to the sugar beet root maggot ...

  20. Climatotherapy at the dead sea for pediatric-onset psoriasis vulgaris.

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    Ben-Amitai, Dan; David, Michael

    2009-01-01

    Climatotherapy at the Dead Sea is highly effective and safe for the treatment of psoriasis vulgaris in adults. We examine the efficacy and safety of climatotherapy at the Dead Sea in children with psoriasis vulgaris. More than 75% improvement in the Psoriasis Area and Severity Index was noted in 35.3% of the patients. None of the patients had side effects.

  1. Cardiac surgery in a patient with pemphigus vulgaris: anesthetic and surgical considerations.

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    Takahashi, Mitsuko; Rhee, Amanda J; Rakasi, Raghuveer R; Yildirim, Asu; Silvay, George; Reddy, Ramachandra C

    2014-03-01

    Pemphigus vulgaris is an autoimmune disorder that causes blistering of the skin and mucous membranes. We present a patient with pemphigus vulgaris who required combined coronary artery bypass grafting and mitral valve repair. The challenges that we faced and modifications to the technique required in this situation are described and reviewed.

  2. [Research on characteristic of interrelationship between toxic organic compound BPA and Chlorella vulgaris].

    Science.gov (United States)

    Chen, Shan-Jia; Chen, Xiu-Rong; Yan, Long; Zhao, Jian-Guo; Zhang, Fei; Jiang, Zi-Jian

    2014-04-01

    The effects of different concentrations of bisphenol A (BPA) on Chlorella vulgaris and removal capacity of BPA by Chlorella vulgaris were investigated. Results showed that a low concentration (0-20 mg x L(-1)) of BPA promoted the growth of Chlorella vulgaris, whereas a relative high concentration (20-50 mg x L(-1)) of BPA inhibited the growth of Chlorella vulgaris, and the inhibition effect was positively correlated with the concentration of BPA. Likewise, a high dose of initial BPA (> 20 mg x L(-1)) led to a decline in the content of chlorephyll a. Chlorella vulgaris had BPA removal capacity when initial BPA concentration ranged from 2 mg x L(-1) to 50 mg x L(-1). There was positive correlation between the removal rate of BPA per cell and initial BPA concentration. The removal rate of BPA was the highest when initial BPA was 50 mg x L(-1), which appeared between lag phase and logarithmic phase.

  3. Nitrogen assimilation by nodulate plants of Phaseolus vulgaris l. and Vigna unguiculata (l. ) walp

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    Neves, M.C.P.; Fernandes, M.S.; Sa, M.F.M. (Universidade Federal Rural do Rio de Janeiro (Brazil). Dept. de Solos)

    1982-05-01

    Under field conditions, the processes of nitrogen assimilation via nitrogenase and nitrate-reductase, the transport and the accumulation of nitrogen in nodulated plants of Phaseolus vulgaris cv. Rio Tibagi and Vigna unguiculata cv. Vita 34 were compared and contrasted. V. unguiculata showed better nodulation than P. vulgaris and consequently had higher rates of nitrogenase activity. The small nodulation of P. vulgaris resulted in greater dependence on soil mineral nitrogen as indicated by the higher rates of nitrate-reductase acitivty compared with V. unguiculata, especially during reproductive stage of growth. The superiority of V. unguiculata in terms of assimilation and remobilization of stored nitrogen resulted in a seed yield 28% greater than that of P. vulgaris. P. vulgaris showed a negative correlation between the nitrate-reductase activity and the ureide content of the sap indicating that the metabolic pathways leading to ureide production operates alternatively to nitrate assimilation.

  4. Potential Health Effects of Enzymatic Protein Hydrolysates from Chlorella vulgaris

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    Mahsa Sedighi

    2016-06-01

    Full Text Available Background and Objective: Chlorella vulgaris is a multi-cellular edible algal species with abundant proteins. Extraction of high value protein fractions for pharmaceutical and nutritional applications can significantly increase the commercial value of microalga biomasses. There is no known report on the anticancer peptides derived from the Chlorella vulgaris abundant protein.Materials and Methods: This study examined the antimicrobial and anticancer effects of peptides from a hydrolyzed Chlorella vulgaris protein with 62 kDa molecular weight. Protein hydrolysis was done by pepsin as a gastrointestinal protease, and was monitored through protein content measurement, sodium dodecyl sulfate polyacrylamide gel electrophoresis, and high performance liquidchromatography measurements. Inhibitory effect of the produced peptides on Escherichia coli cells and breast cancer cell lines was assayed.Results and Conclusion: Hydrolyzed peptides induced a decrease of about 34.1% in the growth of Escherichia coli, and the peptides of 3 to 5 kDa molecular weight had strong impact on the viability of breast cancer cells with IC50 value of 50 μg μl-1. The peptide fractions demonstrating antimicrobial and anti-cancer activities have the potential for use as functional food ingredients for health benefits. These results demonstrate that inexpensive algae proteinscould be a new alternative to produce anticancer peptides.Conflict of interest: The authors declare that there is no conflict of interest.

  5. Efficacy and Safety of Topical Niacinamide for Acne Vulgaris

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    Zeynep Nurhan Saraçoğlu

    2011-06-01

    Full Text Available Background and Design: To investigate the efficacy and safety of topical 4% naicinamide gel cream in the treatment of mild to moderate acne vulgaris and to assess the quality of life of acne patients.Material and Method: Twenty-nine female patients aged 16-38 (mean: 23.57±5.42 years with mild to moderate acne vulgaris who presented in dermatology outpatient clinic were enrolled in the study. All patients applied 4% niacinamide gel cream (Vivatinell-acnecinamide gel cream® on their faces twice daily for eight weeks. The number of lesions (inflammatory and non-inflammatory was counted at 0, 2, 4 and 8 weeks. The side effects (erythema, desquamation, burning and dryness were recorded. The Skindex-29, a quality-of-life measure for patients with skin disease, was administered to the subjects at the beginning and the end of treatment.Results: The decrease in the mean number of inflammatory lesions was statistically significant at the end of the treatment (pre-treatment vs. post-treatment: 12.24 vs. 6.14; p =0.000. However, there was no statistically significant decrease in the number of non-inflammatory lesions at the end of the eight weeks. The niacinamide gel cream was generally well tolerated. There was statistically significant improvement in the Skindex-29 scale scores (p =0.000 at the end of the treatment.Conclusion: Topical 4% niacinamide gel cream may be an alternative treatment for inflammatory lesions of mild to moderate acne vulgaris.

  6. Managing nonteratogenic adverse reactions to isotretinoin treatment for acne vulgaris.

    Science.gov (United States)

    Reilly, Bridget K; Ritsema, Tamara S

    2015-07-01

    Isotretinoin is the strongest, most effective oral treatment for patients with severe acne vulgaris, with remission rates of 89% and higher. Because of its potency, isotretinoin causes many adverse reactions. This article reviews common and severe adverse reactions to isotretinoin and how providers can best manage these reactions. Because of inconclusive research on the correlation between isotretinoin and depression and irritable bowel syndrome, providers should ask patients about symptoms monthly. Prescribing micronized isotretinoin and starting at the lowest dose with gradual upward titration also can help reduce the incidence of adverse reactions.

  7. Monoterpenes from thyme (Thymus vulgaris) as potential mosquito repellents.

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    Park, Byeoung-Soo; Choi, Won-Sik; Kim, Jeong-Han; Kim, Kap-Ho; Lee, Sung-Eun

    2005-03-01

    Five monoterpenes (carvacrol, p-cymene, linalool, alpha-terpinene, and thymol) derived from the essential oil of thyme (Thymus vulgaris) were examined for their repellency against the mosquito Culex pipiens pallens. All 5 monoterpenes effectively repelled mosquitoes based on a human forearm bioassay. Alpha-terpinene and carvacrol showed significantly greater repellency than a commercial formulation, N,N-diethyl-m-methylbenzamide (deet), whereas thymol showed similar repellency to that of deet. The duration of repellency after application for all these monoterpenes was equal to or higher than that of deet. These findings indicate that a spray-type solution containing 2% alpha-terpinene may serve as an alternative mosquito repellent.

  8. Supercritical CO2 extraction of essential oils from Thymus vulgaris

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    S.A.B. Vieira de Melo

    2000-09-01

    Full Text Available Supercritical CO2 extraction of essential oil from Thymus vulgaris leaves was studied using experimental data recently obtained in the Florys S.p.A. laboratory. Mass transfer coefficients in the supercritical and solid phases from extraction curves at 40°C and 20 MPa were evaluated using a mathematical model based on the local adsorption equilibrium of essential oil on lipid in leaves. The adsorption equilibrium constant was fitted to these experimental data, and internal and external mass transfer resistances were calculated, allowing identification of the mechanism controlling the extraction process.

  9. Lupus vulgaris with tubercular lymphadenitis and IgA nephropathy.

    Science.gov (United States)

    Khaira, Ambar; Rathi, Om P; Mahajan, Sandeep; Sharma, Alok; Dinda, Amit K; Tiwari, Suresh C

    2008-02-01

    A 14-year-old girl presented with a 10-year history of a large crusted plaque over the right thigh for 10 years and small reddish plaque over the left upper back for 3 months. On routine evaluation, she was found to have hematuria. Skin biopsy from the lesion was suggestive of skin tuberculosis (lupus vulgaris), and kidney biopsy showed features of IgA nephropathy (IgAN). Fine-needle aspiration from the inguinal lymph node was consistent with granulomatous disease. The patient has been on anti-tubercular treatment, and the hematuria has subsided.

  10. Ulcerative lupus vulgaris over nose, leading to cosmetic deformity.

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    Nair, Pragya A; Mehta, Malay J; Patel, Bhumi B

    2015-01-01

    Lupus vulgaris (LV), is a chronic and progressive form of secondary cutaneous tuberculosis. In India, it is commonly seen over buttocks, thighs, and legs whereas involvement of nose is quite rare. Ulcerative variant particularly over nose causes destruction of cartilage, leading to irreversible deformities and contracture. High-index of suspicion is required for early diagnosis and prevention of cosmetic deformity. A case of LV over nose in a young male with ulceration is reported who responded well to anti-tubercular therapy, but left with scarring of nose, which could have been prevented if adequate awareness regarding extra-pulmonary cases would have been practiced.

  11. Azetidine-2-carboxylic acid in garden beets (Beta vulgaris).

    Science.gov (United States)

    Rubenstein, Edward; Zhou, Haihong; Krasinska, Karolina M; Chien, Allis; Becker, Christopher H

    2006-05-01

    Azetidine-2-carboxylic acid (L-Aze) is a toxic and teratogenic non-protein amino acid. In many species, including man, L-Aze is misincorporated into protein in place of proline, altering collagen, keratin, hemoglobin, and protein folding. In animal models of teratogenesis, it causes a wide range of malformations. The role of L-Aze in human disease has been unexplored, probably because the compound has not been associated with foods consumed by humans. Herein we report the presence of L-Aze in the garden or table beet (Beta vulgaris).

  12. Spina ventosa with lupus vulgaris and lymphadenopathy: Multifocal tuberculosis.

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    Sharma, Sanjeev; Sood, Shikha; Gupta, Mudita

    2015-12-01

    Tuberculous dactylitis is a rare yet well-recognized disease of small bones of the hands and feet. It occurs in young children below five years of age. Tubercular dactylitis with lupus vulgaris and lymphadenopathy was suspected clinically and radiologically in an 8-year-old girl who had multiple soft tissue swelling of hands and feet with ulceration, encrustations, and an atrophic scar with lytic expansile lesions of the small bones of the hands and feet. Tubercular lymph node involvement was confirmed histopathologically.

  13. The Paleobiolinguistics of the Common Bean (Phaseolus vulgaris L.

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    Cecil H. Brown

    2014-10-01

    Full Text Available Paleobiolinguistics is used to determine when and where the common bean (Phaseolus vulgaris L. developed significance for prehistoric groups of Native America. Dates and locations of proto-languages for which common bean terms reconstruct generally accord with crop-origin and dispersal information from plant genetics and archaeobotany. Paleobiolinguistic and other lines of evidence indicate that human interest in the common bean became significant primarily with the widespread development of a village‐farming way of life in the New World rather than earlier when squash and maize and a few other crops became important.

  14. Efecto del AIB y el TDZ en el enraizamiento in vitro de plantas de Bambusa vulgaris var vulgaris Schrad. ex Wendl

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    Yudith García Ramírez

    2012-09-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Título en ingles: Effect of IBA and TDZ on in vitro rooting of plants Bambusa vulgaris var. vulgaris Schrad. ex Wendl. Resumen: Bambusa vulgaris var. vulgaris Schard. ex Wendl. sobresale dentro del género por sus propiedades físico-mecánicas y por el tamaño de sus culmos. Desarrollar la propagación vía organogénesis sería una alternativa para propagar esta especie. Sin embargo, los bajos porcentajes de enraizamiento y de superviviencia ex vitro han sido elementos que han afectado la propagación de diferentes especies de bambúes. Con el objetivo de determinar el efecto de diferentes concentraciones de AIB y TDZ en la emisión de raíces in vitro, se evaluó el número de plantas con raíces, la altura de las plantas (cm desde la base hasta el punto de inserción de la primera hoja y el largo de la raíz (cm. Los resultados demostraron que tanto el AIB como el TDZ influyeron en el enraizamiento in vitro de Bambusa vulgaris var vulgaris. Al adicionar al medio de cultivo TDZ (0,6 mg.L-1 se obtuvieron los mayores porcentajes de formación de raíces (88,2% y estas fueron emitidas mostrando un geotropismo positivo. Palabras clave: bambú; cultivo de tejidos; reguladores de crecimiento. Abstract: Bambusa vulgaris var. vulgaris Schard. ex Wendl. stands out into the genus by its physic - mechanical properties and the culms size. Propagation via organogenesis would be an alternative to propagate this species. However, the low percentages of rooting and ex vitro survival have been elements that have affected the propagation of different bamboo species. The number of plants with roots, plant height (cm (from the base to the insertion point of the first leaf and root length (cm were evaluated in order to determine the effect of different concentrations of IBA and TDZ in the emission of roots in vitro. Results demonstrated that both, AIB and TDZ, influenced the in vitro

  15. 污泥堆肥用于观叶植物银叶菊无土栽培的研究%Study on Soilless Culture of Foliage Plant Senecio cineraria with Sludge Compost

    Institute of Scientific and Technical Information of China (English)

    幸宏伟; 傅敏

    2009-01-01

    [Objective]The study was to screen out the optimum matrix for soilless culture of Senecio cineraria.[Method] With sludge compost, vermiculite and perlite as the materials, the matrix for soilless culture of S. Cineraria was prepared and the effects of different matrix on growth of S. Cineraria were studied.[Result] The growth vigor of crown width and plant height of S. Cineraria in 50% sludge+ 50% (vermiculite +perlite) were the best, the following were those in 60% sludge+40% (vermiculite+ perlite). The growth vigor of leaf length and leaf width of S. Cineraria in 60% sludge +50%( vermiculite+ perlite) was the best, and the contents of N and P in S. Cineraria leaves were the highest. The following were those in 50% sludge+50% (vermiculite+ perlite). The biomass of S. Cineraria in 50% sludge + 50% (vermiculite+ perlite) and 60% sludge+40% (vermiculite+ perlite) were very significantly higher than that in vermiculite+ perlite.[Conclusion] The optimum matrix for soilless culture of S. Cineraria were 50% sludge + 50% (vermiculite+ perlite) and 60% sludge+40%(vermiculite +perlite).%[目的]筛选银叶菊无土栽培的最佳基质.[方法]以污泥堆肥、蛭石和珍珠岩为原料配制银叶菊无土栽培基质,研究不同基质对银叶菊营养生长的影响.[结果]50%污泥+50%(蛭石+珍珠岩)基质中银叶菊冠幅、株高长势最好,60%污泥+40%(蛭石+珍珠岩)次之;60%污泥+40%(蛭石+珍珠岩)基质中银叶菊叶长、叶宽长势最好,叶片N、P含量最高,50%污泥+50%(蛭石+珍珠岩)次之;50%污泥+50%(蛭石+珍珠岩)和60%污泥+40%(蛭石+珍珠岩)基质中银叶菊的生物量极显著高于蛭石+珍珠岩基质中的银叶菊.[结论]银叶菊无土栽培的适宜基质为50%污泥+50%(蛭石+珍珠岩)和60%污泥+40%(蛭石+珍珠岩).

  16. Effects of sodium pentaborate pentahydrate exposure on Chlorella vulgaris growth, chlorophyll content, and enzyme activities.

    Science.gov (United States)

    Chen, Xueqing; Pei, Yuansheng

    2016-10-01

    Sodium pentaborate pentahydrate (SPP) is a rare mineral. In this study, SPP was synthesized from boric acid and borax through low-temperature crystallization, and its effects on the growth of the alga, Chlorella vulgaris (C. vulgaris) were assessed. The newly synthesized SPP was characterized by chemical analysis, X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis, and differential thermal analysis. The changes in C. vulgaris growth, chlorophyll content, and enzyme activities upon exposure to SPP for 168h were evaluated. Results showed that SPP treatment was detrimental to C. vulgaris growth during the first 24-120h of exposure. The harmful effects, however, diminished over time (168h), even at an effective medium concentration of 226.37mg BL(-1) (the concentration of boron applied per liter of culture medium). A similar trend was observed for chlorophyll content (chlorophyll a and b) and indicated that the photosynthesis of C. vulgaris was not affected and that high levels of SPP may even promote chlorophyll synthesis. Superoxide dismutase and catalase activities of C. vulgaris increased during 24-120h exposure to SPP, but these activities gradually decreased as culture time progressed. In other words, the initial detrimental effects of synthetic SPP on C. vulgaris were temporary and reversible. This research provides a scientific basis for applications of SPP in the environment.

  17. A meta-analysis of association between acne vulgaris and Demodex infestation.

    Science.gov (United States)

    Zhao, Ya-E; Hu, Li; Wu, Li-Ping; Ma, Jun-Xian

    2012-03-01

    Until now, etiology of acne vulgaris is still uncertain. Although clinicians usually deny the association between Demodex infestation and acne vulgaris, it has been proved in some clinical practices. To confirm the association between Demodex infestation and acne vulgaris, a meta-analysis was conducted. Predefined selection criteria were applied to search all published papers that analyzed the association between Demodex infestation and acne vulgaris (January 1950 to August 2011) in ISI Web of Knowledge, MEDLINE, and China National Knowledge Infrastructure (CNKI) databases. A meta-analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs) based on fixed effects models or random effects models. We enrolled the 60 Chinese and 3 English papers in this meta-analysis, which covered Turkey and 25 different provinces/municipalities in China and 42130 participants including students and residents, aged from 1 to 78 years. The pooled OR in random effects models is 2.80 (95% CI, 2.34-3.36). Stability is robust according to sensitivity analysis. The fail-safe number is 18477, suggesting that at least 18477 articles with negative conclusions would be needed to reverse the conclusion that acne vulgaris was related to Demodex infestation. So the effect of publication bias was insignificant and could be ignored. It was concluded that acne vulgaris is associated with Demodex infestation. This indicates that when regular treatments for acne vulgaris are ineffective, examination of Demodex mites and necessary acaricidal therapies should be considered.

  18. Inhibitory effects of Iranian Thymus vulgaris extracts on in vitro growth of Entamoeba histolytica.

    Science.gov (United States)

    Behnia, Maryam; Haghighi, Ali; Komeylizadeh, Hossein; Tabaei, Seyyed-Javadi Seyyed; Abadi, Alireza

    2008-09-01

    One of the most common drugs used against a wide variety of anaerobic protozoan parasites is metronidazole. However, this drug is mutagenic for bacteria and is a potent carcinogen for rodents. Thymus vulgaris is used for cough suppression and relief of dyspepsia. Also it has antibacterial and antifungal properties. The aim of this study was to investigate antiamebic effect of Thymus vulgaris against Entamoeba histolytica in comparison with metronidazole. One hundred gram air-dried T. vulgaris plant was obtained and macerated at 25 degrees C for 14 days using n-hexane and a mixture of ethanol and water. For essential oil isolation T. vulgaris was subjected to hydrodistillation using a clevenger-type apparatus for 3 hr. E. histolytica, HM-1: IMSS strain was used in all experiments. It was found that the minimal inhibitory concentration (MIC) for T. vulgaris hydroalcoholic, hexanic extracts, and the essential oil after 24 hr was 4 mg/mL, 4 mg/mL, and 0.7 mg/mL, respectively. After 48 hr the MIC for T. vulgaris hydroalcoholic and hexanic extracts was 3 and 3 mg/mL, respectively. Therefore, it can be concluded that the Iranian T. vulgaris is effective against the trophozoites of E. histolytica.

  19. Inhibitory effect ofThymus vulgaris extract on memory impairment induced by scopolamine in rat

    Institute of Scientific and Technical Information of China (English)

    Zahra Rabiei; Shiva Mokhtari; Samira Asgharzade; Mostafa Gholami; Samira Rahnama; Mahmoud Rafieian-kopaei

    2015-01-01

    Objective:To investigate the effect ofThymus vulgaris (T. vulgaris) on learning and memory functions in scopolamine-induced memory deficit in rats. Memory enhancing activity in scopolamine-induced amnesic rats was investigated by assessing the Morris water maze and passive avoidance paradigm. Methods:A total of 42 male Wistar rats were divided into 6 equal groups as follow: control group: received water, scopolamine treated group: received scopolamine 1 mg/kg for 15 days, two scopolamine+T. vulgaris treated groups: received scopolamine andT. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days, two intact groups: receivedT. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days. Results: Administration ofT. vulgaris extract significantly restored memory and learning impairments induced by scopolamine in the passive avoidance test and Morris water maze test. Conclusions:T. vulgaris extract has repairing effects on memory and behavioral disorders produced by scopolamine and may have beneficial effects in the treatment of Alzheimer's disease.

  20. Inhibitory effect of Thymus vulgaris extract on memory impairment induced by scopolamine in rat简

    Institute of Scientific and Technical Information of China (English)

    Zahra; Rabiei; Shiva; Mokhtari; Samira; Asgharzade; Mostafa; Gholami; Samira; Rahnama; Mahmoud; Rafieian-kopaei

    2015-01-01

    Objective: To investigate the effect of Thymus vulgaris(T. vulgaris) on learning and memory functions in scopolamine-induced memory deficit in rats. Memory enhancing activity in scopolamine-induced amnesic rats was investigated by assessing the Morris water maze and passive avoidance paradigm.Methods: A total of 42 male Wistar rats were divided into 6 equal groups as follow:control group: received water, scopolamine treated group: received scopolamine 1 mg/kg for 15 days, two scopolamine + T. vulgaris treated groups: received scopolamine and T. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days, two intact groups:received T. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days.Results: Administration of T. vulgaris extract significantly restored memory and learning impairments induced by scopolamine in the passive avoidance test and Morris water maze test.Conclusions: T. vulgaris extract has repairing effects on memory and behavioral disorders produced by scopolamine and may have beneficial effects in the treatment of Alzheimer’s disease.

  1. In Vitro and In Vivo Anti-inflammatory Effects of Struthanthus vulgaris.

    Science.gov (United States)

    Marques, Franciane Martins; da Costa, Maycow Rodrigues; Vittorazzi, Cátia; Gramma, Luciane De Souza Dos Santos; Barth, Thiago; de Andrade, Tadeu Uggere; Endringer, Denise Coutinho; Scherer, Rodrigo; Fronza, Marcio

    2017-06-01

    Struthanthus vulgaris is probably the most common medicinal mistletoe plant in Brazil, and has been used in folk medicine as an anti-inflammatory agent and for cleaning skin wounds. Our proposal was to evaluate the anti-inflammatory activity of S. vulgaris ethanol leaf extract and provide further insights of how this biological action could be explained using in vitro and in vivo assays. In vitro anti-inflammatory activity was preliminarily investigated in lipopolysaccharide/interferon gamma-stimulated macrophages based on their ability to inhibit nitric oxide production and tumor necrosis factor-alpha. In vivo anti-inflammatory activity of S. vulgaris ethanol leaf extract was investigated in the mice carrageenan-induced inflammation air pouch model. The air pouches were inoculated with carrageenan and then treated with 50 and 100 mg/kg of S. vulgaris ethanol leaf extract or 1 mg/kg of dexamethasone. Effects on the immune cell infiltrates, pro- and anti-inflammatory mediators such as tumor necrosis factor-alpha, interleukin 1, interleukin 10, and nitric oxide, were evaluated. The chemical composition of S. vulgaris ethanol leaf extract was characterized by LC-MS/MS. In vitro S. vulgaris ethanol leaf extract significantly decreased the production of nitric oxide and tumor necrosis factor-alpha in macrophages and did not reveal any cytotoxicity. In vivo, S. vulgaris ethanol leaf extract significantly suppressed the influx of leukocytes, mainly neutrophils, protein exudation, nitric oxide, tumor necrosis factor-alpha, and interleukin 1 concentrations in the carrageenan-induced inflammation air pouch. In conclusion, S. vulgaris ethanol leaf extract exhibited prominent anti-inflammatory effects, thereby endorsing its usefulness as a medicinal therapy against inflammatory diseases, and suggesting that S. vulgaris ethanol leaf extract may be a source for the discovery of novel anti-inflammatory agents. Georg Thieme Verlag KG Stuttgart · New York.

  2. Quality of life, self-esteem and psychosocial factors in adolescents with acne vulgaris*

    Science.gov (United States)

    Vilar, Gustavo Nunes; dos Santos, Laís Araújo; Sobral Filho, Jader Freire

    2015-01-01

    Background Dermatological diseases, among which acne vulgaris, have psychological impact on the affected generating feelings of guilt, shame and social isolation. Objectives To compare quality of life, self-esteem and other psychosocial variables amongst adolescents with and without acne vulgaris, and between levels of severity. Methods Cross-sectional observational study in a sample of 355 high school students from the city of João Pessoa. Data collection was performed with questionnaires and clinical-dermatological evaluation. The primary variables were the incidence of AV; quality of life, set by the Children's Dermatology Quality of Life Index and Dermatology Quality of Life Index; and self-esteem, measured by the Rosenberg Self-Esteem Scale. For calculation of statistical tests, we used the SPSS 20.0 software, considering p=0.05. Results The sample, with an average age of 16, showed 89.3% prevalence of acne vulgaris. The most prevalent psychosocial issue was "afraid that acne will never cease", present in 58% of affected youth. The median score of Quality of Life in Children's Dermatology Index was different amongst students with and without acne vulgaris (p=0.003), as well as the Quality of Life in Dermatology (p=0.038) scores, so that students with acne vulgaris have worse QoL. There was a correlation between the severity of acne vulgaris and worse quality of life. Self-esteem was not significantly associated with the occurrence or severity of acne vulgaris. Conclusions acne vulgaris assumes significance in view of its high prevalence and the effect on quality of life of adolescents, more severe at the more pronounced stages of disease (p<0.001). The psychosocial impact of acne vulgaris should be valued in the management of patients with this condition. PMID:26560206

  3. Quality of life, self-esteem and psychosocial factors in adolescents with acne vulgaris.

    Science.gov (United States)

    Vilar, Gustavo Nunes; Santos, Laís Araújo dos; Sobral Filho, Jader Freire

    2015-01-01

    Dermatological diseases, among which acne vulgaris, have psychological impact on the affected generating feelings of guilt, shame and social isolation. To compare quality of life, self-esteem and other psychosocial variables amongst adolescents with and without acne vulgaris, and between levels of severity. Cross-sectional observational study in a sample of 355 high school students from the city of João Pessoa. Data collection was performed with questionnaires and clinical-dermatological evaluation. The primary variables were the incidence of AV; quality of life, set by the Children's Dermatology Quality of Life Index and Dermatology Quality of Life Index; and self-esteem, measured by the Rosenberg Self-Esteem Scale. For calculation of statistical tests, we used the SPSS 20.0 software, considering p=0.05. The sample, with an average age of 16, showed 89.3% prevalence of acne vulgaris. The most prevalent psychosocial issue was "afraid that acne will never cease", present in 58% of affected youth. The median score of Quality of Life in Children's Dermatology Index was different amongst students with and without acne vulgaris (p=0.003), as well as the Quality of Life in Dermatology (p=0.038) scores, so that students with acne vulgaris have worse QoL. There was a correlation between the severity of acne vulgaris and worse quality of life. Self-esteem was not significantly associated with the occurrence or severity of acne vulgaris. acne vulgaris assumes significance in view of its high prevalence and the effect on quality of life of adolescents, more severe at the more pronounced stages of disease (p<0.001). The psychosocial impact of acne vulgaris should be valued in the management of patients with this condition.

  4. Treatment of acne vulgaris with fractional radiofrequency microneedling.

    Science.gov (United States)

    Kim, Sang Tae; Lee, Kang Hoon; Sim, Hyung Jun; Suh, Kee Suck; Jang, Min Soo

    2014-07-01

    Fractional radiofrequency microneedling is a novel radiofrequency technique that uses insulated microneedles to deliver energy to the deep dermis at the point of penetration without destruction of the epidermis. It has been used for the treatment of various dermatological conditions including wrinkles, atrophic scars and hypertrophic scars. There have been few studies evaluating the efficacy of fractional radiofrequency microneedling in the treatment of acne, and none measuring objective parameters like the number of inflammatory and non-inflammatory acne lesions or sebum excretion levels. The safety and efficacy of fractional radiofrequency microneedling in the treatment of acne vulgaris was investigated. In a prospective clinical trial, 25 patients with moderate to severe acne were treated with fractional radiofrequency microneedling. The procedure was carried out three times at 1-month intervals. Acne lesion count, subjective satisfaction score, sebum excretion level and adverse effects were assessed at baseline and at 4, 8 and 12 weeks after the first treatment as well as 4, 8 and 12 weeks after the last treatment. Number of acne lesions (inflammatory and non-inflammatory) decreased. Sebum excretion and subjective satisfaction were more favorable at every time point compared with the baseline values (P acne vulgaris.

  5. The electron transfer system of syntrophically grown Desulfovibrio vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Walker, C.B.; He, Z.; Yang, Z.K.; Ringbauer, Jr., J.A.; He, Q.; Zhou, J.; Voordouw, G.; Wall, J.D.; Arkin, A.P.; Hazen, T.C.; Stolyar, S.; Stahl, D.A.

    2009-05-01

    Interspecies hydrogen transfer between organisms producing and consuming hydrogen promotes the decomposition of organic matter in most anoxic environments. Although syntrophic couplings between hydrogen producers and consumers are a major feature of the carbon cycle, mechanisms for energy recovery at the extremely low free energies of reactions typical of these anaerobic communities have not been established. In this study, comparative transcriptional analysis of a model sulfate-reducing microbe, Desulfovibrio vulgaris Hildenborough, suggested the use of alternative electron transfer systems dependent upon growth modality. During syntrophic growth on lactate with a hydrogenotrophic methanogen, D. vulgaris up-regulated numerous genes involved in electron transfer and energy generation when compared with sulfate-limited monocultures. In particular, genes coding for the putative membrane-bound Coo hydrogenase, two periplasmic hydrogenases (Hyd and Hyn) and the well-characterized high-molecular weight cytochrome (Hmc) were among the most highly expressed and up-regulated. Additionally, a predicted operon coding for genes involved in lactate transport and oxidation exhibited up-regulation, further suggesting an alternative pathway for electrons derived from lactate oxidation during syntrophic growth. Mutations in a subset of genes coding for Coo, Hmc, Hyd and Hyn impaired or severely limited syntrophic growth but had little affect on growth via sulfate-respiration. These results demonstrate that syntrophic growth and sulfate-respiration use largely independent energy generation pathways and imply that understanding of microbial processes sustaining nutrient cycling must consider lifestyles not captured in pure culture.

  6. The Electron Transfer System of Syntrophically Grown Desulfovibrio vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    PBD; ENIGMA; GTL; VIMSS; Walker, Christopher B.; He, Zhili; Yang, Zamin K.; Ringbauer Jr., Joseph A.; He, Qiang; Zhou, Jizhong; Voordouw, Gerrit; Wall, Judy D.; Arkin, Adam P.; Hazen, Terry C.; Stolyar, Sergey; Stahl, David A.

    2009-06-22

    Interspecies hydrogen transfer between organisms producing and consuming hydrogen promotes the decomposition of organic matter in most anoxic environments. Although syntrophic couplings between hydrogen producers and consumers are a major feature of the carbon cycle, mechanisms for energy recovery at the extremely low free energies of reactions typical of these anaerobic communities have not been established. In this study, comparative transcriptional analysis of a model sulfate-reducing microbe, Desulfovibrio vulgaris Hildenborough, suggested the use of alternative electron transfer systems dependent upon growth modality. During syntrophic growth on lactate with a hydrogenotrophic methanogen, D. vulgaris up-regulated numerous genes involved in electron transfer and energy generation when compared with sulfate-limited monocultures. In particular, genes coding for the putative membrane-bound Coo hydrogenase, two periplasmic hydrogenases (Hyd and Hyn) and the well-characterized high-molecular weight cytochrome (Hmc) were among the most highly expressed and up-regulated. Additionally, a predicted operon coding for genes involved in lactate transport and oxidation exhibited up-regulation, further suggesting an alternative pathway for electrons derived from lactate oxidation during syntrophic growth. Mutations in a subset of genes coding for Coo, Hmc, Hyd and Hyn impaired or severely limited syntrophic growth but had little affect on growth via sulfate-respiration. These results demonstrate that syntrophic growth and sulfate-respiration use largely independent energy generation pathways and imply that understanding of microbial processes sustaining nutrient cycling must consider lifestyles not captured in pure culture.

  7. Recently approved systemic therapies for acne vulgaris and rosacea.

    Science.gov (United States)

    Del Rosso, James Q

    2007-08-01

    Until recently, with the exception of oral isotretinoin for the treatment of severe recalcitrant nodular acne, systemic therapy for acne vulgaris and rosacea has been based on anecdotal support, clinical experience, and small clinical trials. Tetracycline derivatives are the predominant systemic agents that have been used for both disease states, prescribed in dose ranges that produce antibiotic activity. Anti-inflammatory dose doxycycline, a controlled-release (CR) 40-mg capsule formulation of doxycycline that is devoid of antibiotic activity when administered once daily, was US Food and Drug Administration (FDA)-approved for the treatment of inflammatory lesions (papules and pustules) of rosacea, based on large-scale phase 3 pivotal trials and long-term microbiologic and safety data. Also, an extended-release (ER) tablet formulation of minocycline was approved by the FDA for the treatment of inflammatory lesions of moderate to severe acne vulgaris in patients 12 years and older based on large-scale phase 3 clinical trials that evaluated efficacy and safety, dose-response analysis, and long-term data. This article discusses the studies and clinical applications related to the use of these agents.

  8. Measurement of bone mineral density in patients with psoriasis vulgaris

    Directory of Open Access Journals (Sweden)

    Nurgül Köse

    2013-12-01

    Full Text Available Objective: The aim of this study was to determine thechanges of bone mineral density in patients with psoriasisvulgaris.Methods: 33 patients, diagnosed with psoriasis vulgarisin Erciyes University, Medical School, Department of Dermatology,and 23 healthy subjects as control group wereincluded in this study. Lumbar and femur bone mineraldensity of all patients and control group were measured.Biochemical tests related to bone metabolism were performed.Results: We did not find statistically significant differencebetween patient and control groups’ bone mineral density.However, the lumbar spine t score of men, were found tobe significantly lower in the patient group than the controlgroup (p<0.05. There was no statistically significant differencein bone mineral density of women between thepatient and control groups. The effects on bone mineraldensity of disease period and using the topical corticosteroidswere not statistically significant.Conclusion: Our study indicates that bone mineral densityof patients with psoriasis vulgaris was not differentfrom healthy subject.Key words: Psoriasis vulgaris, bone mineral density, osteoporosis

  9. Antimicrobial susceptibility of Propionibacterium acnes isolated from acne vulgaris.

    Science.gov (United States)

    Kurokawa, I; Nishijima, S; Kawabata, S

    1999-01-01

    Systemic and topical antimicrobial treatment for acne vulgaris remains the mainstay method of therapy in Japan. Strains of Propionibacterium acnes (P. acnes) resistant to erythromycin (EM), clindamycin (CLDM), tetracycline (TC), doxycycline (DOXY) and minocycline (MINO) have been reported. The aim of the present study was to examine the antimicrobial susceptibility to 10 currently used antimicrobial agents of 50 strains of P. acnes isolated from acne lesions and identified using a Rap ID ANA II panel. Minimum inhibitory concentrations (MIC) were determined by the agar dilution method according to the criteria of the Japan Society of Chemotherapy. EM, ampicillin (ABPC), and CLDM were the most potent drugs, followed by MINO, nadifloxacin (NDFX), cephalexin (CEX), DOXY, ofloxacin (OFLX), and TC. In terms of the MIC80, EM and ABPC were the most potent, followed by CLDM, NDFX, MINO, CEX, DOXY, OFLX, TC and gentamycin (GM). Although most of the strains used were susceptible to the antimicrobial agents tested, strains of P. acnes resistant (MIC 12.5 mug/ml) to EM (4%), CLDM (4%), DOXY (2%) and TC (2%) were observed. In this study, no strains of P. acnes resistant to MINO were seen, suggesting that oral MINO is the most useful treatment for acne vulgaris with minimal risk of bacterial resistance.

  10. Stability and loading properties of curcumin encapsulated in Chlorella vulgaris.

    Science.gov (United States)

    Jafari, Yaser; Sabahi, Hossein; Rahaie, Mahdi

    2016-11-15

    Curcumin (Cur), a polyphenols with pharmacological function, was successfully encapsulated in algae (Alg) cell (Chlorella vulgaris) as confirmed by fluorescence microscopy, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and Fourier transform-infrared spectroscopy (FT-IR). Fluorescence micrographs, TGA, DSC and FTIR spectra suggested the hypothesis inclusion Cur in Nano-empty spaces inside cell wall of Alg. The TGA analysis showed that the thermal stability of Alg and Cur at algae/curcumin complex was 3.8% and 33% higher than their free forms at 0-300°C and 300-600°C ranges, respectively. After encapsulation in Alg cells, the photostability of Cur was enhanced by about 2.5-fold. Adsorption isotherm of Cur into Alg was fitted with the Freundlich isotherm. The microcapsules were loaded with Cur up to about 55% w/w which is much higher than other reported bio-carriers. In conclusion, the data proved that Chlorella vulgaris cell can be used as a new stable carrier for Cur.

  11. Hubungan Konsumsi Makanan Cepat Saji Terhadap Kejadian Akne Vulgaris pada Mahasiswa FK USU Stambuk 2007

    OpenAIRE

    Bancin, Berry Eka Parda

    2011-01-01

    Introduction. Acne vulgaris is a very common disease found in public, especially in adolescence. About 85% of people in their life have had acne vulgaris, so it’s often assumed as a physiological state. High-calorie foods have been long suspected as one of the causes of this disease, this opinion is supported by the increased incidence of acne vulgaris in developing countries today. Since 1946 to 2007 various studies have been conducted on the relationship of food to the occurrence of acne vu...

  12. Detection of filaggrin gene mutation (2282del4) in Pakistani Ichthyosis vulgaris families.

    Science.gov (United States)

    Naz, Naghma; Samdani, Azam Jah

    2011-06-01

    The aim of this study was to detect an 811 bp filaggrin (FLG) gene fragment known to carry a mutation 2282del4 which causes ichthyosis vulgaris. Seven clinically examined ichthyosis vulgaris families were included in this study. An 811 bp FLG gene fragment was targeted in the genomic DNA of all the members of the seven families by PCR amplification using known primers RPT1P7 and RPT2P1. Successful amplification of an 811 bp FLG gene fragment in all the families suggested the possible role of the 2282del4 mutation in causing ichthyosis vulgaris in Pakistani population.

  13. Terms referring to psoriasis vulgaris in the classical Chinese medicine literature: a systematic analysis.

    Science.gov (United States)

    Zhang, Claire Shuiqing; May, Brian; Yan, Yuhong; Yu, Jason Jingjie; Yao, Danni; Chang, Suyueh; Zhang, Anthony Lin; Guo, Xinfeng; Lu, Chuanjian; Xue, Charlie Changli

    2016-04-01

    Psoriasis vulgaris is a modern medical term. In the classical Chinese medicine (CM) literature, this disorder could be classified under a diversity of terms. In order to explore how psoriasis vulgaris was conceptualized and managed throughout Chinese medical history, we undertook a systematic longitudinal analysis of descriptions in the classical CM literature of skin disorders consistent with psoriasis vulgaris. Candidate search terms were identified from 33 contemporary CM books as relevant to psoriasis vulgaris. Thirteen terms were used to search the Zhong Hua Yi Dian--'Encyclopaedia of Traditional Chinese Medicine', 4th edition, a digital collection of over 1000 medical books. Search results were entered in a spreadsheet, citations that were consistent with psoriasis vulgaris were identified based on: (i) a description of the features of psoriasis vulgaris and (ii) judgment by two clinicians regarding relevance to psoriasis vulgaris. Analysis focused on the use of terms by historical period (Chinese dynasty) and whether the terms were specific for psoriasis vulgaris 608 citations dating from 363 to 1947AD were included. The two criteria for determining inclusion of citations were combined to select the pool of citations most relevant to psoriasis vulgaris. Sixty citations from eight search terms were found in this pool. Among the eight terms, Bai Bi, She Shi and Bi Feng consistently identified citations that were likely to be psoriasis. The earliest was from 1368AD (Ming dynasty). The remaining five terms yielded inconsistent results. Citations of disorders consistent with psoriasis vulgaris appear prior to 1368-1644AD (Ming dynasty), but the terms used were not specific for psoriasis. Bai Bi, She Shi and Bi Feng are the terms most consistently used to refer to psoriasis vulgaris. They first appeared in 1368-1644AD (Ming dynasty) and Bai bi remains in use. Regarding the other terms, certain citations may have referred to psoriasis vulgaris, but in other cases

  14. Changes in hemostasis in foals naturally infected with Strongylus vulgaris

    DEFF Research Database (Denmark)

    Pihl, Tina Holberg; Krarup Nielsen, Martin; Jacobsen, Stine

    2017-01-01

    enzyme-linked immunosorbent assay values in all foals using a linear mixed effects model with repeated measures and to total numbers of S. vulgaris larvae in nine foals at necropsy with Pearson linear correlation. In the first week of life, all evaluated indices of hemostasis were significantly different...... 4. Strongylus vulgaris antibody levels were statistically associated with D-dimer (P = .0076) and fibrinogen (P = .0004) concentrations. Naturally acquired infection with S. vulgaris was associated with changes suggestive of mild activation of coagulation, fibrinolysis, and inflammation. The results...

  15. Perbandingan Konsumsi Lemak Berdasarkan Tingkat Keparahan Akne Vulgaris pada Siswa SMK Negeri 1 Kota Jambi

    Directory of Open Access Journals (Sweden)

    Nisa Sulistia

    2015-09-01

    Full Text Available Abstrak Salah satu faktor pencetus akne vulgaris adalah diet tinggi lemak, terutama lemak jenuh. Tujuan penelitian ini adalah untuk mengetahui perbandingan konsumsi lemak berdasarkan tingkat keparahan akne vulgaris menurut kriteria Lehmann. Penelitian ini berupa studi analitik observasional dengan desain cross sectional melalui pemeriksaan statusdermatologikus menurut kriteria Lehmann dan pengambilan data konsumsi menggunakan FFQ (Food Frequency Questionnaire pada 138 siswa kelas X dan XI SMK Negeri 1 Kota Jambi. Hasil penelitian menunjukkan terdapat akne vulgaris derajat ringan (49%, akne vulgaris derajat sedang (42%, dan akne vulgaris derajat berat (9%. Reratakonsumsi lemak total 89,35 ± 17,63 gram. Rerata konsumsi SFA (Saturated Fatty Acid 37,07 ± 9,97 gram. Rerata konsumsi MUFA (Monounsaturated Fatty Acid 15,30 ± 11,79 gram. Rerata konsumsi PUFA (Polyunsaturated Fatty Acid 12,03 ± 9,25 gram.Uji oneway Anova menunjukkan tidak ada perbedaan bermakna rerata konsumsi lemak total (p > 0,05 dan SFA (p > 0,05 berdasarkan tingkat keparahan akne vulgaris. Uji Kruskal-Wallis menunjukkan tidak ada perbedaan bermakna MUFA (p > 0,05, dan PUFA (p > 0,05 berdasarkan tingkat keparahan akne vulgaris. Penelitian ini menunjukkan tidak ada perbedaan konsumsi lemak berdasarkan tingkat keparahan akne vulgaris pada siswa SMKNegeri 1 Kota Jambi.Kata kunci: akne vulgaris, FFQ, lemak total, SFA, MUFA, PUFA Abstract One of the precipited factors is high fat diet, especially saturated fats. The objective of this study was to determine the comparison of fat consumption which is based on the severity of acne vulgaris.This research wasanalytic observational study using cross sectional design, by examining dermatologic status according to Lehmann criteria and  taking consumption record with FFQ (Food Frequency Questionnaire of  138 tenth and eleventh grade student in SMKN 1 Jambi. The result showed that mild acne vulgaris (49%, moderate acne vulgaris (42%, and

  16. Shelf-life extension of minimally processed and gamma irradiated red beet (Beta vulgaris ssp. vulgaris L.), Cv. early wonder

    Energy Technology Data Exchange (ETDEWEB)

    Hernandes, Nilber Kenup; Vital, Helio de Carvalho [Centro Tecnologico do Exercito (CTEx), Rio de Janeiro, RJ (Brazil). Div. de Defesa Quimica, Biologica e Nuclear (DDQBN)]. E-mail: nilberkenup@ctex.eb.br; vital@ctex.eb.br; Coneglian, Regina Celi Cavestre [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, Rio de Janeiro, RJ (Brazil). Inst.de Agronomia. Dept. de Fitotecnia]. E-mail: rccconeg@ufrrj.br; Godoy, Ronoel Luiz de Oliveira [EMBRAPA Agroindustria de Alimentos, Rio de Janeiro, RJ (Brazil)]. E-mail: ronoel@ctaa.embrapa.br

    2007-07-01

    This work investigated the effects of gamma irradiation on the shelf-life extension and safety of minimally processed red beet (Beta vulgaris ssp. vulgaris L.) by performing microbiological, chemical and sensory analyses. Red beets were harvested 73 days after transplanting and their tuberous parts were minimally processed and separated in two groups: control (non-irradiated) and irradiated (0.5, 1.0 and 1.5 kGy). Tests for Salmonella sp., total and fecal coliforms, total count of aerobic mesophilic and lactic-acid bacteria were performed during the 21-day storage at 8 deg C. They indicated that the samples irradiated with 1.0 and 1.5 kGy remained in good conditions throughout storage while the unirradiated samples did not last 7 days. Chemical analyses indicated that the concentrations of vitamins B1 and B2 were not affected by irradiation. In contrast the amounts of fructose and glucose increased during storage while the one for sucrose decreased. In addition four series of sensory evaluations including appearance and aroma indicated that the samples irradiated with 1.0 and 1.5 kGy remained good for consumption for 20 days. Therefore it was concluded that the use of the doses of 1.0 and 1.5 kGy produced the best effects on the conservation of the samples without harming the sensory characteristics and nutritional constituents tested. (author)

  17. Genetic resources of Thymus vulgaris L. and T. vulgaris x T. Marschallianus Willd. in the Czech Republic

    Directory of Open Access Journals (Sweden)

    Duskova, Elena

    2016-07-01

    Full Text Available Two varieties of Thymus vulgaris L. (´Krajový´ and ´Winter´ and three its hybrids with T. Marschallianus Will. (variety ´Mixta´ and two accessions of variety ´Lemona´ were evaluated according the Draft Descriptor List Thymus vulgaris L. and analysed for the essential oil content and composition in years 2014 and 2015. All evaluated accessions were found morphologically and/or chemically different. Varieties ´Krajový´, ´Winter´ and ´Mixta´ were assessed as a thymol type with the thymol (39.1 – 69.6 %, o-cymene (6.3 – 24.9 % and γ-terpinene (2.75 – 13.8 % as main oil components. The two accessions of ´Lemona´ variety were found significantly different each other: one of them (income No. 3239 was assessed as a terpineol type with the terpineol acetate (75.7 % and α-terpineol (16.9 % as main oil components and the other one (income No. 2757 as a geraniol type with the geranyl acetate (±42.4 % and geraniol (±20.8 % as main oil components. Only the accession with income No. 3239 was proved as a ´Lemona´ variety due its citral (±9.3 % content, though even this content is too small compare to original ´Lemona´ where about 20 % of citral was declared.

  18. An alternative to estimate the atmospheric carbon sequestration by Bambusa vulgaris var. vulgaris Schrad. ex J.C. Wendl.

    Directory of Open Access Journals (Sweden)

    Alicia Mercadet

    2011-07-01

    Full Text Available The estimation of atmospheric carbon sequestration by a woody plant has two alternatives for implementation: by identifying the average annual increase in volume or by determining the appropriate method for calculating the volume stand. The aim of this work was to obtain a reliable and simple method for estimating the carbon in plants and culms of bamboo (Bambusa vulgaris var. vulgaris existing in a territory. Single samples were taken without replacement in different areas and each culm was measured in total length, outside diameter and wall thickness every two meters from the base to the segment in the upper left to present an interior cavity identifiable, in which the remaining length to the top was measured. Graphical analyses were conducted with the data collected of trends in relations between the variables considered along the culm. By regression analysis with different models and the use of determination coefficient (R2 as an adjustment indicator, we selected those that best allow estimating the solid volume of the culm. After obtaining the calibration models and their results, we prepared a spreadsheet that facilitates to take down the values of the different variables by culm and scion as well as the characterization of the scion average culm. The results are a first attempt to develop a method for estimating the carbon sequestered by the bamboo culm at both as scion and territory to include this element among the criteria for the management plans of this non-wood forest resource. Key words: allometric models, bamboo, regression analysis

  19. Primer registro de Vespula vulgaris (Hymenoptera: Vespidae en la Argentina First record of Vespula vulgaris (Hymenoptera: Vespidae in Argentina

    Directory of Open Access Journals (Sweden)

    Maité Masciocchi

    2010-12-01

    Full Text Available Vespula vulgaris (Linnaeus es un véspido social nativo de la región Holártica. En este trabajo reportamos la primera detección de esta especie en Argentina. Obreras de esta avispa fueron capturadas cerca de la ciudad de San Carlos de Bariloche (Argentina en Febrero de 2010, mientras se tomaban muestras de otra avispa invasora, Vespula germanica (Fabricius o chaqueta amarilla, de morfología externa y hábitos similares a la anteriormente mencionada. Además, detallamos algunos caracteres de identificación y características biológicas.Vespula vulgaris (Linnaeus is a social vespid native to the Holarctic region. The first detection of this species in Argentina is here reported. Workers were captured close to San Carlos de Bariloche (Argentina during February 2010, while sampling for another successful invader, the German wasp or Yellowjacket, Vespula germanica (Fabricius. Both these wasp species are very similar morphologically and share a number of common habits. Also, some identification features and biological characters are here explained.

  20. Rhizofiltration using sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) to remediate uranium contaminated groundwater

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Minhee, E-mail: heelee@pknu.ac.kr [Department of Environmental Geosciences, Pukyong National University, 599-1 Daeyondong, Namgu, Busan 608-737 (Korea, Republic of); Yang, Minjune [Department of Environmental Geosciences, Pukyong National University, 599-1 Daeyondong, Namgu, Busan 608-737 (Korea, Republic of)

    2010-01-15

    The uranium removal efficiencies of rhizofiltration in the remediation of groundwater were investigated in lab-scale experiments. Sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) were cultivated and an artificially uranium contaminated solution and three genuine groundwater samples were used in the experiments. More than 80% of the initial uranium in solution and genuine groundwater, respectively, was removed within 24 h by using sunflower and the residual uranium concentration of the treated water was lower than 30 {mu}g/L (USEPA drinking water limit). For bean, the uranium removal efficiency of the rhizofiltration was roughly 60-80%. The maximum uranium removal via rhizofiltration for the two plant cultivars occurred at pH 3-5 of solution and their uranium removal efficiencies exceeded 90%. The lab-scale continuous rhizofiltration clean-up system delivered over 99% uranium removal efficiency, and the results of SEM and EDS analyses indicated that most uranium accumulated in the roots of plants. The present results suggested that the uranium removal capacity of two plants evaluated in the clean-up system was about 25 mg/kg of wet plant mass. Notably, the removal capacity of the root parts only was more than 500 mg/kg.

  1. Rhizofiltration using sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) to remediate uranium contaminated groundwater.

    Science.gov (United States)

    Lee, Minhee; Yang, Minjune

    2010-01-15

    The uranium removal efficiencies of rhizofiltration in the remediation of groundwater were investigated in lab-scale experiments. Sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) were cultivated and an artificially uranium contaminated solution and three genuine groundwater samples were used in the experiments. More than 80% of the initial uranium in solution and genuine groundwater, respectively, was removed within 24h by using sunflower and the residual uranium concentration of the treated water was lower than 30 microg/L (USEPA drinking water limit). For bean, the uranium removal efficiency of the rhizofiltration was roughly 60-80%. The maximum uranium removal via rhizofiltration for the two plant cultivars occurred at pH 3-5 of solution and their uranium removal efficiencies exceeded 90%. The lab-scale continuous rhizofiltration clean-up system delivered over 99% uranium removal efficiency, and the results of SEM and EDS analyses indicated that most uranium accumulated in the roots of plants. The present results suggested that the uranium removal capacity of two plants evaluated in the clean-up system was about 25mg/kg of wet plant mass. Notably, the removal capacity of the root parts only was more than 500 mg/kg.

  2. Mixotrophic growth and biochemical analysis of Chlorella vulgaris cultivated with diluted monosodium glutamate wastewater.

    Science.gov (United States)

    Ji, Yan; Hu, Wenrong; Li, Xiuqing; Ma, Guixia; Song, Mingming; Pei, Haiyan

    2014-01-01

    Monosodium glutamate wastewater (MSGW) is a potential medium for microbial cultivation because of containing abundant organic nutrient. This paper seeks to evaluate the feasibility of growing Chlorella vulgaris with MSGW and assess the influence of MSGW concentration on the biomass productivity and biochemical compositions. The MSGW diluted in different concentrations was prepared for microalga cultivation. C. vulgaris growth was greatly promoted with MSGW compared with the inorganic BG11 medium. C. vulgaris obtained the maximum biomass concentration (1.02 g/L) and biomass productivity (61.47 mg/Ld) with 100-time diluted MSGW. The harvested biomass was rich in protein (36.01-50.64%) and low in lipid (13.47-25.4%) and carbohydrate (8.94-20.1%). The protein nutritional quality and unsaturated fatty acids content of algal increased significantly with diluted MSGW. These results indicated that the MSGW is a feasible alternative for mass cultivation of C. vulgaris.

  3. Influence of plaque-forming bacterium, Rhodobacteraceae sp. on the growth of Chlorella vulgaris.

    Science.gov (United States)

    Chen, Zhangran; Zhang, Jingyan; Lei, Xueqian; Zhang, Bangzhou; Cai, Guanjing; Zhang, Huajun; Li, Yi; Zheng, Wei; Tian, Yun; Xu, Hong; Zheng, Tianling

    2014-10-01

    Experiments were conducted to find out the molecular features, infection process of a special alga plaque-forming microorganism and its potential influence on the biomass of Chlorella vulgaris during the infection process. Direct contact between the algal cell and the bacterium may be the primary steps needed for the bacterium to lyse the alga. Addition of C. vulgaris cells into f/2 medium allowed us obtain the object bacterium. The 16S rRNA gene sequence comparisons results showed that the plaque-forming bacterium kept the closest relationship with Labrenzia aggregata IAM 12614(T) at 98.90%. The existence of the bacterium could influence both the dry weight and lipid content of C. vulgaris. This study demonstrated that direct cell wall disruption of C. vulgaris by the bacterium would be a potentially effective method to utilize the biomass of microalgae.

  4. Th-17 and the lack of efficacy of ustekinumab in pemphigus vulgaris.

    Science.gov (United States)

    Tirado-Sánchez, Andrés; Ponce-Olivera, Rosa María; Vazquez-González, Denisse; Bonifaz, Alexandro

    2013-03-15

    Systemic corticosteroids represent an effective treatment for pemphigus vulgaris (PV). However, this treatment is related to many adverse side effects. Herein, we report a case of PV treated with ustekinumab.

  5. Cranial Mesenteric Arterial Obstruction Due To Strongylus vulgaris Larvae in a Donkey (Equus asinus.

    Directory of Open Access Journals (Sweden)

    Hassan Borji

    2014-09-01

    Full Text Available Arteritis due to Strongylus vulgaris is a well-known cause of colic in horses and donkeys. The current report describes a fatal incidence of arterial obstruction in cranial mesenteric artery caused by S. vulgaris infection in an adult donkey in which anthelmintic treatment was not regularly administered. Necropsy findings of the abdominal cavity revealed a complete cranial mesenteric arterial obstruction due to larvae of S. vulgaris, causing severe colic. To the authors' knowledge, a complete cranial mesenteric arterial obstruction due to verminous arteritis has rarely been described in horses and donkeys. Based on recent reports of fatal arterial obstruction due to S. vulgaris infection in donkeys, it may be evident to consider acute colic caused by this pathogenic parasite a re-emerging disease in donkeys and horses.

  6. Composition and antimicrobial studies of essential oil of Thymus vulgaris from Montenegro

    Directory of Open Access Journals (Sweden)

    Damjanović-Vratnica, B.

    2015-12-01

    Full Text Available Chemical composition of the hydrodistilled essential oil of Thymus vulgaris L. (thyme from Montenegro was analyzed by gas chromatography-mass spectrometry and its antimicrobial activity was evaluated against 10 microorganisms, including reference and clinically isolated strains. T. vulgaris essential oil yield was 0.42% (v/w, based on the dry leaves weight whereas the analysis showed that major components, amongst 22 identified in the oil, were geraniol (25.66%, geranyl-acetate (20.34%, linalool (10.89% and caryophyllene oxide (9.89%. The results of the antimicrobial activity tests revealed that the essential oil of T .vulgaris from Montenegro has rather strong antimicrobial activity, especially against Staphylococcus aureus, Escherichia coli, Candida albicans and Klebsiella pneumoniae. These results confirm the potential use of T. vulgaris essential oil in food products the as well as for therapeutic applications.

  7. Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts.

    Science.gov (United States)

    Al-Bayati, Firas A

    2008-03-28

    Essential oils (EOs) and methanol extracts obtained from aerial parts of Thymus vulgaris and Pimpinella anisum seeds were evaluated for their single and combined antibacterial activities against nine Gram-positive and Gram-negative pathogenic bacteria: Staphylococcus aureus, Bacillus cereus, Escherichia coli, Proteus vulgaris, Proteus mirabilis, Salmonella typhi, Salmonella typhimurium, Klebsiella pneumoniae and Pseudomonas aeruginosa. The essential oils and methanol extracts revealed promising antibacterial activities against most pathogens using broth microdilution method. Maximum activity of Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts (MIC 15.6 and 62.5mug/ml) were observed against Staphylococcus aureus, Bacillus cereus and Proteus vulgaris. Combinations of essential oils and methanol extracts showed an additive action against most tested pathogens especially Pseudomonas aeruginosa.

  8. Lupus Vulgaris Erythematoides: report of a patient initially misdiagnosed as dermatitis.

    Science.gov (United States)

    Vazquez-Lopez, Francisco; Fueyo-Casado, Alejandro; Gonzalez-Lara, Leire

    2013-05-15

    A small percentage of patients with tuberculosis present with cutaneous findings, which may be difficult to diagnose. We present a patient diagnosed with a rare, non-scarring form of cutaneous tuberculosis (CTB), classically termed as lupus vulgaris erythematoides.

  9. Induction of tryptophanase in short cells and swarm cells of Proteus vulgaris.

    OpenAIRE

    Hoffman, P S; Falkinham, J O

    1981-01-01

    Tryptophanase was noninducible in swarm cells of Proteus vulgaris despite transport of the inducer tryptophan. Further, cyclic AMP, which stimulated increased levels of tryptophanase in short cells, had no effect on swarm cells.

  10. Octopus vulgaris (Cuvier, 1797) in the Mediterranean Sea: Genetic Diversity and Population Structure: e0149496

    National Research Council Canada - National Science Library

    Daniele De Luca; Gaetano Catanese; Gabriele Procaccini; Graziano Fiorito

    2016-01-01

      The common octopus, Octopus vulgaris Cuvier 1797, is a largely exploited cephalopod species in the Mediterranean Sea and the Atlantic Ocean, as well as along the coasts of Africa, Brazil and Japan...

  11. Transcriptome analysis of salt tolerant common bean (Phaseolus vulgaris L.) under saline conditions

    National Research Council Canada - National Science Library

    Hiz, Mahmut Can; Canher, Balkan; Niron, Harun; Turet, Muge

    2014-01-01

    .... Common bean, Phaseolus vulgaris L., a major protein source in developing countries, is highly affected by soil salinity and the information on genes that play a role in salt tolerance is scarce...

  12. Serological diagnosis of Strongylus vulgaris infection: use of a recombinant protein

    DEFF Research Database (Denmark)

    Andersen, Ulla Vestergaard; Howe, Daniel K.; Olsen, Susanne Nautrup

    , an immunoreactive cDNA clone was subcloned into E. coli and the plasmid sequenced, the open reading frame encoding the mature protein was cloned into a pET22b expression vector and expressed as a His-tagged recombinant protein in BL21 expression cells. The recombinant protein was used in an indirect enzyme...... the pathogenic migrating larval stages of S. vulgaris ante mortem. A cDNA library was constructed from RNA extracted from migrating S. vulgaris larvae. Excretory-secretory antigens from S. vulgaris adult specimens were used to immunise a rat. Hyperimmune serum was used to immunoscreen the cDNA library....... vulgaris (n=9) reacted against the recombinant protein, expressed as optic density (OD) readings of >24 % of a positive control, while sera from negative horses had OD readings

  13. Medicinal Plants for the Treatment of Acne Vulgaris: A Review of Recent Evidences

    OpenAIRE

    Nasri, Hamid; Bahmani, Mahmoud; Shahinfard, Najmeh; Moradi Nafchi, Atefeh; Saberianpour, Shirin; Rafieian Kopaei, Mahmoud

    2015-01-01

    Context: Acne vulgaris affects about 85% of teenagers and may continue to adulthood. There are about two million visits to physicians per year for teenagers and the direct cost of acne treatment in the US exceeds $1 billion per year. Evidence Acquisition: A wide variety of treatment regimens exist for acne vulgaris including benzoil peroxide, retinoids, isotretinoids, keratolytic soaps, alpha hydroxy acids, azelaic acid, salicilic acid as well as hormonal, anti-androgen or antiseborrheic trea...

  14. Clinical cross-reactivity between Artemisia vulgaris and Matricaria chamomilla (chamomile).

    Science.gov (United States)

    de la Torre Morín, F; Sánchez Machín, I; García Robaina, J C; Fernández-Caldas, E; Sánchez Triviño, M

    2001-01-01

    Artemisia vulgaris is a common weed and an important source of allergens on the subtropical island of Tenerife, Canary Islands, Spain. It pollinates mainly from July to September, although, due to some local climatic conditions, it may flower throughout the year. Cross-reactivity with hazelnut, kiwi, birch, several Compositae (Ambrosia, Chrysanthemum, Matricaria, Solidago) and grass allergens has been suggested. Few studies have addressed the issue of in vivo cross-reactivity between A. vulgaris and Matricaria chamomilla. The objective of this study was to perform conjunctival and bronchial challenges with A. vulgaris and M. chamomilla and oral challenge with chamomile in 24 patients with asthma and/or rhinitis sensitized primarily to A. vulgaris. Skin prick tests with M. chamomilla were positive in 21 patients. Eighteen patients had a positive conjunctival provocation test with a A. vulgaris pollen extract and 13 patients had a positive conjunctival provocation test with a M. chamomilla pollen extract. Bronchial provocation tests with A. vulgaris were positive in 15 patients and with M. chamomilla pollen in another 16 individuals. Oral provocation tests, conducted with a commercial chamomile infusion were positive in 13 patients. Nine of these individuals were skin test positive to food allergens and 17 to others pollens of the Compositae family. This study confirms a high degree of in vivo cross-reactivity between A. vulgaris and M. chamomilla. Sensitization to A. vulgaris seems to be a primary risk factor for experiencing symptoms after the ingestion of chamomile infusions. Based on the results of bronchial provocation tests, M. chamomilla pollen could be a relevant inhalant allergen.

  15. Clinical characteristics and epidermal barrier function of papulopustular rosacea: A comparison study with acne vulgaris

    OpenAIRE

    Zhou, Maosong; Xie, Hongfu; Cheng, Lin; Li, Ji

    2016-01-01

    Objective: To evaluate the clinical characteristics and epidermal barrier function of papulopustular rosacea by comparing with acne vulgaris. Methods: Four hundred and sixty-three papulopustular rosacea patients and four hundred and twelve acne vulgaris patients were selected for the study in Xiangya Hospital of Central South University from March 2015 to May 2016. They were analyzed for major facial lesions, self-conscious symptoms and epidermal barrier function. Results: Erythema, burning, ...

  16. The genome sequence of Barbarea vulgaris facilitates the study of ecological biochemistry

    DEFF Research Database (Denmark)

    Byrne, Stephen L.; Erthmann, Pernille Østerbye; Agerbirk, Niels

    2017-01-01

    The genus Barbarea has emerged as a model for evolution and ecology of plant defense compounds, due to its unusual glucosinolate profile and production of saponins, unique to the Brassicaceae. One species, B. vulgaris, includes two ‘types’, G-type and P-type that differ in trichome density, and t...... deter larvae to the extent that they die. The B. vulgaris genome will promote the study of mechanisms in ecological biochemistry to benefit crop resistance breeding....

  17. Identification of an Alternative to Proteus vulgaris as a Laboratory Standard for Hydrogen Sulfide Production

    Directory of Open Access Journals (Sweden)

    Nar'Asha Randall

    2017-05-01

    Full Text Available This project involved the evaluation of a biosafety level 1 alternative to Proteus vulgaris as a positive control for the production of hydrogen sulfide. We determined that Citrobacter freundii could serve as an excellent substitute for P. vulgaris, and that lead acetate strips used in conjunction with triple sugar iron media allows for consistent results following evaluation after up to one week.

  18. XML Investigating the Phytochemical, Antibacterial and Antifungal Effects of Thymus Vulgaris and Cuminum Cyminum Essential Oils

    OpenAIRE

    2016-01-01

    Background and Objective: The aim of this study was to determine the chemical composition, antibacterial and antifungal effects of Thymus vulgaris and Cuminum Cyminum essential oils against foodborne pathogens and Candida species in vitro. Methods: The essential oils were extracted from the aerial parts of Thymus vulgaris and dried Cuminum Cyminum seeds using a Clevenger apparatus for 3 hours. Analysis of the essential oils’ constituents was performed using gas chromatography-mass s...

  19. DRY CALCAREOUS GRASSLAND COMMUNITIES (FILIPENDULA VULGARIS-HELICTOTRICHON PRATENSE) IN WESTERN AND CENTRAL LATVIA

    OpenAIRE

    S. RUSINA

    2003-01-01

    The dry calcareous grassland vegetation of Westem and Central Latvia is described based on 93 rclevés, Ali relevés could be assigned to one community type Filipendula vulgaris-Helictotrichon pratense named according to dominant species. Four variants were distinguished: typicum, Viscaria vulgaris, Astragalus danicus and Carex flacca. Ellenberg indìcator values were calculated to study the ecology of communities. Floristic differences among variants are associated mainly w...

  20. Dispersal of Calluna vulgaris (L. Hull. seeds on severely burnt upland moorland

    Directory of Open Access Journals (Sweden)

    J.A. Gilbert

    2010-07-01

    Full Text Available From natural and manipulated experiments at 460 m a.s.l., traps were used to record the dispersal range of Calluna vulgaris seeds on an unvegetated post-wildfire site. Seeds were trapped to 50 m from source, although most (73% were deposited within one metre of the parent plant. C. vulgaris seeds were not dispersed as far in these experiments as recorded in previous workers’ experiments on lowland grassland.

  1. DRY CALCAREOUS GRASSLAND COMMUNITIES (FILIPENDULA VULGARIS-HELICTOTRICHON PRATENSE) IN WESTERN AND CENTRAL LATVIA

    OpenAIRE

    Rusina, S.

    2003-01-01

    The dry calcareous grassland vegetation of Westem and Central Latvia is described based on 93 rclevés, Ali relevés could be assigned to one community type Filipendula vulgaris-Helictotrichon pratense named according to dominant species. Four variants were distinguished: typicum, Viscaria vulgaris, Astragalus danicus and Carex flacca. Ellenberg indìcator values were calculated to study the ecology of communities. Floristic differences among variants are associa...

  2. Therapeutic plasma exchange-A new dawn in the treatment of pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Rajesh Kumar

    2015-01-01

    Full Text Available Pemphigus vulgaris is an autoimmune disorder that involves intraepithelial blistering and sores of skin and mucous membrane. It generally correlates with the levels of circulating autoantibodies; their removal seems a reasonable therapeutic approach. Therapeutic plasma exchange is hypothesized to remove pathogenic autoantibodies and has been used in refractory or severe cases. It may be considered for rapid control of severe or recalcitrant pemphigus vulgaris and should be combined with use of concomitant immuno-suppressive.

  3. Two-Component Signal Transduction Systems of Desulfovibrio Vulgaris: Structural and Phylogenetic Analysis and Deduction of Putative Cognate Pairs

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Weiwen; Culley, David E.; Wu, Gang; Brockman, Fred J.

    2006-01-20

    ABSTRACT-Two-component signal transduction systems (TCSTS) composed of sensory histidine kinases (HK) and response regulators (RR), constitute a key element of the mechanism by which bacteria sense and respond to changes in environments. A large number of TCSTSs including 59 putative HKs and 55 RRs were identified from the Desulfovibrio vulgaris genome, indicating their important roles in regulation of cellular metabolism. In this study, the structural and phylogenetic analysis of all putative TCSTSs in D. vulgaris was performed. The results showed D. vulgaris contained an unexpectedly large number of hybrid-type HKs, implying that multiple-step phosphorelay may be a common signal transduction mechanism in D. vulgaris. Most TCSTS components of D. vulgaris were found clustered into several subfamilies previously recognized in other bacteria and extensive co-evolution between D. vulgaris HKs and RRs was observed, suggesting that the concordance of HKs and RRs in cognate phylogenetic groups could be indicative of cognate TCSTSs...

  4. Study of the effect of extract of Thymus vulgaris on anxiety in male rats.

    Science.gov (United States)

    Komaki, Alireza; Hoseini, Faeghe; Shahidi, Siamak; Baharlouei, Negar

    2016-07-01

    There is some evidence in traditional medicine for the effectiveness of Thymus vulgaris ( bǎi lǐ xiāng) in the treatment of anxiety in humans. The elevated plus-maze (EPM) has broadly been used to investigate anxiolytic and anxiogenic compounds. The present study investigated the effects of extract of T. vulgaris on rat behavior in the EPM. In the present study, the data were obtained from male Wistar rats. Animals were divided into four groups: saline group and T. vulgaris groups (50 mg/kg, 100 mg/kg, and 200 mg/kg infusion for 7 days by feeding). During the test period, the total distance covered by animals, the number of open- and closed-arm entries, and the time spent in open and closed arms of the EPM were recorded. T. vulgaris increased open-arm exploration and open-arm entry in the EPM, whereas extract of this plant has no effects on the total distance covered by animals and the number of closed-arm entries. The results of the present experiment indicate that T. vulgaris may have an anxiolytic profile in rat behavior in the EPM test, which is not influenced by the locomotor activity. Further research is required to determine the mechanisms by which T. vulgaris extract exerts an anxiolytic effect in rats.

  5. Prevalence and risk factors of facial acne vulgaris among Chinese adolescents.

    Science.gov (United States)

    Wu, Tie-Qang; Mei, Shu-Qing; Zhang, Jin-Xin; Gong, Long-Fen; Wu, Fen-Jin; Wu, Wei-Hong; Li, Jun; Lin, Min; Diao, Jian-Xing

    2007-01-01

    Acne vulgaris, a very common condition among adolescents, can result in psychological distress, including anxiety and depression. The purpose of this study was to assess the prevalence and risk factors of acne vulgaris among Chinese adolescents. In the Zhou Hai district of Guangdong Province, 3163 students 10 to 18 years old were selected from 7 schools. Information was collected using self-administrated questionnaIres and physician examinations. The prevalence of acne vulgaris was calculated from the collected data. Potential risk factors including age, gender, diet, skin type, sleeping habits, and facial make-up use were analyzed using stepwise logistic regression. The results showed a prevalence of acne vuglaris of 53.5% in all adolescents, with 51.3% in males and 58.6% in females. The prevalence of inflammatory acne in males and females combined was 25.8% and of acne scarring 7.1%. Increased age was related to higher prevalence and severity of acne vulgaris: 15.6%, 44.9%, and 70.4% for 10, 13, and 16 year olds, respectively. Acne vulgaris was more prevalent in girls under and boys over 14 years of age. Significant risk factors of acne vuglaris included age, skin type (oily, mixed, or neutral skin in comparison with dry skin), insufficient sleep, and cosmetic make-up use. Acne vulgaris is prevalent among Chinese adolescents 10 to 18 years old. Health education to address this condition in Chinese adolescents could have important implications for public health.

  6. Polyphosphate during the Regreening of Chlorella vulgaris under Nitrogen Deficiency.

    Science.gov (United States)

    Chu, Fei-Fei; Shen, Xiao-Fei; Lam, Paul K S; Zeng, Raymond J

    2015-09-28

    Polyphosphate (Poly-P) accumulation has been reported in Chlorella vulgaris under nitrogen deficiency conditions with sufficient P supply, and the process has been demonstrated to have great impact on lipid productivity. In this article, the utilization of polyphosphates and the regreening process under N resupplying conditions, especially for lipid production reviving, were investigated. This regreening process was completed within approximately 3-5 days. Polyphosphates were first degraded within 3 days in the regreening process, with and without an external P supply, and the degradation preceded the assimilation of phosphate in the media with an external P offering. Nitrate assimilation was markedly influenced by the starvation of P after polyphosphates were exhausted in the medium without external phosphates, and then the reviving process of biomass and lipid production was strictly impeded. It is, thus, reasonable to assume that simultaneous provision of external N and P is essential for overall biodiesel production revival during the regreening process.

  7. Polyphosphate during the Regreening of Chlorella vulgaris under Nitrogen Deficiency

    Directory of Open Access Journals (Sweden)

    Fei-Fei Chu

    2015-09-01

    Full Text Available Polyphosphate (Poly-P accumulation has been reported in Chlorella vulgaris under nitrogen deficiency conditions with sufficient P supply, and the process has been demonstrated to have great impact on lipid productivity. In this article, the utilization of polyphosphates and the regreening process under N resupplying conditions, especially for lipid production reviving, were investigated. This regreening process was completed within approximately 3–5 days. Polyphosphates were first degraded within 3 days in the regreening process, with and without an external P supply, and the degradation preceded the assimilation of phosphate in the media with an external P offering. Nitrate assimilation was markedly influenced by the starvation of P after polyphosphates were exhausted in the medium without external phosphates, and then the reviving process of biomass and lipid production was strictly impeded. It is, thus, reasonable to assume that simultaneous provision of external N and P is essential for overall biodiesel production revival during the regreening process.

  8. Cannibalistic behavior of octopus (Octopus vulgaris) in the wild.

    Science.gov (United States)

    Hernández-Urcera, Jorge; Garci, Manuel E; Roura, Alvaro; González, Angel F; Cabanellas-Reboredo, Miguel; Morales-Nin, Beatriz; Guerra, Angel

    2014-11-01

    The first description of cannibalism in wild adult Octopus vulgaris is presented from 3 observations made in the Ría de Vigo (NW Spain), which were filmed by scuba divers. These records document common traits in cannibalistic behavior: (a) it was intercohort cannibalism; (b) attacks were made by both males and females; (c) in 2 of the records, the prey were transported to the den, which was covered with stones of different sizes; (d) the predator started to eat the tip of the arms of its prey; (e) predation on conspecifics occurred even if there were other abundant prey available (i.e., mussels); and (f) the prey/predator weight ratio in the 3 cases ranged from 20% to 25% body weight. The relationships between this behavior and sex, defense of territory, energy balance, food shortage, competition and predation, as well as how the attacker kills its victim are discussed.

  9. Physiological and biochemical responses of Chlorella vulgaris to Congo red.

    Science.gov (United States)

    Hernández-Zamora, Miriam; Perales-Vela, Hugo Virgilio; Flores-Ortíz, César Mateo; Cañizares-Villanueva, Rosa Olivia

    2014-10-01

    Extensive use of synthetic dyes in many industrial applications releases large volumes of wastewater. Wastewaters from dying industries are considered hazardous and require careful treatment prior to discharge into receiving water bodies. Dyes can affect photosynthetic activities of aquatic flora and decrease dissolved oxygen in water. The aim of this study was to evaluate the effect of Congo red on growth and metabolic activity of Chlorella vulgaris after 96h exposure. Exposure of the microalga to Congo red reduced growth rate, photosynthesis and respiration. Analysis of chlorophyll a fluorescence emission showed that the donor side of photosystem II was affected at high concentrations of Congo red. The quantum yield for electron transport (φEo), the electron transport rate (ETR) and the performance index (PI) also decreased. The reduction in the ability to absorb and use the quantum energy increased non-photochemical (NPQ) mechanisms for thermal dissipation. Overall, Congo red affects growth and metabolic activity in photosynthetic organisms in aquatic environments.

  10. Pemphigus Vulgaris Confined to the Gingiva: A Case Report

    Directory of Open Access Journals (Sweden)

    Mitsuhiro Ohta

    2011-01-01

    Full Text Available Pemphigus Vulgaris (PV is an autoimmune intraepithelial blistering disease involving the skin and mucous membranes. Oral mucosa is frequently affected in patients with PV, and oral lesions may be the first sign of the disease in majority of patients. In some patients, oral lesions may also be followed by skin involvement. Therefore, timely recognition and therapy of oral lesions is critical as it may prevent skin involvement. Early oral lesions of PV are, however, often regarded as difficult to diagnose, since the initial oral lesions may be relatively nonspecific, manifesting as superficial erosions or ulcerations, and rarely presenting with the formation of intact bullae. Lesions may occur anywhere on the oral mucosa including gingiva; however; desquamtive gingivitis is less common with PV than other mucocutaneous conditions such as pemphigoid or lichen planus. This paper describes the case of a patient presenting with a one-year history of painful gingival, who is finally diagnosed as having PV.

  11. A Novel Rhodopsin Gene from Octopus vulgaris for Optobioelectronics Materials

    Directory of Open Access Journals (Sweden)

    Alexander Zhgun

    2015-06-01

    Full Text Available The unique photochromic retinal protein from rhabdomeric octopus membranes – octopus rhodopsin (OctR has emerged as promising material for biomolecular photonic applications due to its unique properties and advantages. Here we report isolation of the novel full length octR gene from retina cDNA of Octopus vulgaris eyes and its sequence comparison with rhodopsins of other cephalopods and vertebrates. The isolated gene can be used to develop various expression systems for production of recombinant OctR for structural studies and novel optobioelectronic applications. The alignment of amino acid (a.a. sequence with different opsins revealed similarity to cephalopoda rhodopsins (Rho and to human melanopsin from intrinsically photosensitive retinal ganglion cells. The alingment of OctR a.a. sequence with mammalian and cephalopoda Rho with known 3D structures revealed promising substitutions V2C and W292C for developing stable and functionally active recombinant OctR after heterologous expression.

  12. Antibacterial activity of Beta vulgaris L. pomace extract

    Directory of Open Access Journals (Sweden)

    Velićanski Aleksandra S.

    2011-01-01

    Full Text Available Antibacterial activity of Beta vulgaris L. (beetroot pomace extract (concentration 100 mg/ml was tested against five Gram positive and seven Gram negative bacterial strains (reference cultures and natural isolates. Disc diffusion method with 15 µl of extract and agar-well diffusion method with 50 and 100 µl were used. Antibiotic (cefotaxime/clavulanic acid was used as a control sample. The tested extract showed the highest activity against Staphylococcus aureus and Bacillus cereus, where clear zones (without growth appeared. There was no any activity against other tested Gram-positive bacteria, except for Staphylococcus epidermidis, with a small zone of reduced growth. Growth of all tested Gram-negative bacteria was inhibited usually with 100 µl of extract. The most susceptible were Citrobacter freundii and Salmonella typhymurium. The tested antibiotic gave clear, usually large zones for all tested strains except for Staphylococcus cohni spp. cohni, where only a zone of reduced growth appeared.

  13. Treatment Of ACNE Vulgaris With New Polyherbal Formulations

    Directory of Open Access Journals (Sweden)

    Sachidananda Y N

    2001-01-01

    Full Text Available One hundred and five patients with active lesions of acne vulgaris were included in the open clinical trial. All the patients were administered tablets containing multiple herbal drugs (purim, at a dose of 2 tablets twice daily for four weeks. Simultaneously, they were instructed to apply A herbal cream (Clarina twice daily on the affected area of acne lesion twice daily for four weeks. The response to treatment was excellent in mild to moderate acne (Grade I & II after 4 weeks of treatment. In severe acne with large papules and pustules, the response was also significantly good, in healing the papules and pustules. There were no local or systemic side effects seen in all these patients. Thus, this polyherbal cream (Clarina along with a polyherbal tablet (Purim tablets was useful in treating patients with various degrees of acne.

  14. Oxidation of Octopus vulgaris hemocyanin by nitrogen oxides

    Energy Technology Data Exchange (ETDEWEB)

    Salvato, B.; Giacometti, G.M.; Beltramini, M.; Zilio, F.; Giacometti, G.; Magliozzo, R.S.; Peisach, J.

    1989-01-24

    The reaction of Octopus vulgaris hemocyanin with nitrite was studied under a variety of conditions in which the green half-met derivative is formed. Analytical evidence shows that the amount of chemically detectable nitrite in various samples of the derivative is not proportional to the cupric copper detected by EPR. The kinetics of oxidation of hemocyanin as a function of protein concentration and pH, in the presence of nitrite and ascorbate, is consistent with a scheme in which NO/sub 2/ is the reactive oxidant. We suggest that the green half-methemocyanin contains a metal center with one cuprous and one cupric copper without an exogenous nitrogen oxide ligand.

  15. Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Rajeev, Lara; Luning, Eric; Dehal, Paramvir; Joachimiak, Marcin; Mukhopadhyay, Aindrila

    2010-05-17

    D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that the target was enriched from sheared genomic DNA before proceeding to the DAP-chip.

  16. Volatile compounds of dry beans (Phaseolus vulgaris L.).

    Science.gov (United States)

    Oomah, B Dave; Liang, Lisa S Y; Balasubramanian, Parthiba

    2007-12-01

    Volatile compounds of uncooked dry bean (Phaseolus vulgaris L.) cultivars representing three market classes (black, dark red kidney and pinto) grown in 2005 were isolated with headspace solid phase microextraction (HS-SPME), and analyzed with gas chromatography mass spectrometry (GC-MS). A total of 62 volatiles consisting of aromatic hydrocarbons, aldehydes, alkanes, alcohols and ketones represented on average 62, 38, 21, 12, and 9 x 10(6) total area counts, respectively. Bean cultivars differed in abundance and profile of volatiles. The combination of 18 compounds comprising a common profile explained 79% of the variance among cultivars based on principal component analysis (PCA). The SPME technique proved to be a rapid and effective method for routine evaluation of dry bean volatile profile.

  17. Nootropic Effects of Filipendula Vulgaris Moench Water Extract Fractions.

    Science.gov (United States)

    Shilova, I V; Suslov, N I; Amelchenko, V P

    2015-07-01

    Nootropic activity of water extract fractions from aerial parts of Filipendula vulgaris Moench was demonstrated on the models of hermetic volume hypoxia, conditioned passive avoidance response, open field test, and forced swimming with a load. The fractions stimulated hypoxic resistance, normalized orientation and exploratory behavior, improved conditioned response reproduction during testing after hypoxic injury, and increased exercise tolerance. Fractionation of the extract led to dissociation of the effect components, which suggests that individual constituents have specific characteristics. Ethylacetate fraction exhibited most pronounced nootropic activity and was superior to plant extract by some characteristics. The detected effects seemed to be caused by modulation of the hippocampus activity the under the effects of phenol and triterpene compounds.

  18. Injury response of Phaseolus vulgaris to ozone flux density

    Science.gov (United States)

    Amiro, B. D.; Gillespie, T. J.; Thurtell, G. W.

    This study describes a quantitative relationship between mean O 3 flux density and the length of exposure needed for the occurrence of visual injury to Phaseolus vulgaris L. Similar relationships were found for 14 day old and 6 week old plants using a whole leaf gas exchange cuvette system. Cultivars Seafarer (O 3 sensitive) and Gold Crop (O 3 resistant) exhibited similar responses at flux densities > 3 mg m -2 h -1 but only Seafarer was injured below this flux density. O 3 concentration and length of exposure period alone did not contain sufficient information to describe the onset of visual foliar injury. The use of O 3 concentrations in excess of normal ambient conditions compensated for low leaf conductances so that flux densities in the cuvette were similar to those found in the field.

  19. Lichen striatus associated with psoriasis vulgaris treated with oral acitretin

    Directory of Open Access Journals (Sweden)

    Enzo Errichetti

    2014-01-01

    Full Text Available Lichen striatus (LS is an uncommon dermatosis of unknown etiology that presents as a continuous or interrupted linear band of pink, tan, red or skin-colored papules in a blaschkoid distribution. The lesions are generally solitary and unilateral, but unusual extensive cases with multiple and bilateral lesions have been also described. Albeit LS is typically an asymptomatic and self-limited dermatosis, it may cause a significant psychological distress in some patients, thus requiring an appropriate therapy. Topical steroid is the most commonly used treatment but it is not always effective. We report a case of LS unresponsive to topical steroid therapy associated with psoriasis vulgaris successfully treated with oral acitretin.

  20. PRODUCTIVITY OF MICROALGAE CHLORELLA VULGARIS IN LABORATORY CONDITION

    Directory of Open Access Journals (Sweden)

    Agnieszka Patyna

    2017-06-01

    Full Text Available Algae biomass is increasingly regarded as a potential resource that could be used to produce biofuels, electricity and heat. Algae contain a lot of nutrients, so they can be used as food for humans and livestock. Because of their valuable composition (many nutrients they are used as supplements of balanced diet, in turn taking into account their biosorption abbility they are used to detoxification of human body. Algae cultivation does not demand large areas of land to expose cells to sunlight, so their production rate is higher than vascular plants. Moreover algae cultivation lets to achieve high biomass concentration. Important cultivation factors are: illumination (light intensity is an important factor because it drives photosynthesis, CO2 supply, culture medium and mixing. The experimental research was conducted using Chlorella vulgaris BA 002 strain. The aim of this study was to determine the effectiveness of biomass growth in laboratory condition.

  1. Thermogravimetric analysis of the gasification of microalgae Chlorella vulgaris.

    Science.gov (United States)

    Figueira, Camila Emilia; Moreira, Paulo Firmino; Giudici, Reinaldo

    2015-12-01

    The gasification of microalgae Chlorella vulgaris under an atmosphere of argon and water vapor was investigated by thermogravimetric analysis. The data were interpreted by using conventional isoconversional methods and also by the independent parallel reaction (IPR) model, in which the degradation is considered to happen individually to each pseudo-component of biomass (lipid, carbohydrate and protein). The IPR model allows obtaining the kinetic parameters of the degradation reaction of each component. Three main stages were observed during the gasification process and the differential thermogravimetric curve was satisfactorily fitted by the IPR model considering three pseudocomponents. The comparison of the activation energy values obtained by the methods and those found in the literature for other microalgae was satisfactory. Quantification of reaction products was performed using online gas chromatography. The major products detected were H2, CO and CH4, indicating the potential for producing fuel gas and syngas from microalgae.

  2. Oral pemphigus vulgaris: A case report with direct immunofluorescence study

    Science.gov (United States)

    Kumar, Sangeetha Jeevan; Nehru Anand, SP; Gunasekaran, Nandhini; Krishnan, Rajkumar

    2016-01-01

    Pemphigus vulgaris (PV) is a chronic, autoimmune, intraepidermal blistering disease of the skin and mucous membranes. The initial clinical manifestation is frequently the development of intraoral lesions, and later, the lesions involve the other mucous membranes and skin. The etiology of this disease still remains obscure although the presence of autoantibodies is consistent with an autoimmune disease. These antibodies are targeted against the adhesion proteins of keratinocytes, leading to acantholysis (disruption of spinous layer, leading to intraepidermal clefting) and blister formation. Because only oral lesions are present initially, the chances of misdiagnosing the disease as another condition are increased, leading to inappropriate therapy. In this article, we report a case of PV with only oral manifestations in a 36-year-old male. PMID:27721634

  3. Four cases of recalcitrant pemphigus vulgaris salvaged with rituximab

    Directory of Open Access Journals (Sweden)

    Samyak Ganjre

    2017-01-01

    Full Text Available Although the long-term use of immunosuppressives – supplemented with more aggressive treatments such as immunoadsorption, intravenous immunoglobulins, or plasmapheresis in recalcitrant cases has dramatically improved the prognosis of pemphigus vulgaris, opportunistic infections secondary to immunosuppression continue to cause significant mortality. We report four cases– three old ones, who had accumulated significant morbidities over their disease duration ranging from 5 to 10 years, and the fourth, a teenage female intolerant to corticosteroids and idiosyncratic to methotrexate– who achieved complete remission on administration of rituximab by the lymphoma protocol. One of the old cases who had recalcitrant mucositis experienced its complete subsidence without any adjuvant whatsoever. All continue to remain asymptomatic for 11–20 months. None had infusion reactions or any delayed side effects.

  4. Future therapies for pemphigus vulgaris: Rituximab and beyond.

    Science.gov (United States)

    Huang, Amy; Madan, Raman K; Levitt, Jacob

    2016-04-01

    The conventional treatment for patients with pemphigus vulgaris (PV) centers on global immunosuppression, such as the use of steroids and other immunosuppressive drugs, to decrease titers of antidesmoglein autoantibodies responsible for the acantholytic blisters. Global immunosuppressants, however, cause serious side effects. The emergence of anti-CD20 biologic medications, such as rituximab, as an adjunct to conventional therapy has shifted the focus to targeted destruction of autoimmune B cells. Next-generation biologic medications with improved modes of delivery, pharmacology, and side effect profiles are constantly being developed, adding to the diversity of options for PV treatment. We review promising monoclonal antibodies, including veltuzumab, obinutuzumab (GA-101), ofatumumab, ocaratuzumab (AME-133v), PRO131921, and belimumab.

  5. Uric acid : A new antioxidant in patients with pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Maryam Yousefi

    2011-01-01

    Full Text Available Background: Increased reactive oxygen species (ROS and lipid peroxidation are seen in many dermatologic disorders, for example, atopic dermatitis, psoriasis, vitiligo, acne vulgaris, pemphigus vulgaris (PV, lichen planus, and alopecia areata. ROS has an important role in the inflammation process. In PV, increased production of ROS leads to decline of antioxidants in plasma and red blood cells which results in oxidative stress. We aimed to evaluate the level of these antioxidants in PV patients and compare it to the controls. Materials and Methods: Among patients attending the dermatology clinics, 30 patients with PV, who had never been on treatment, were enrolled to the study. The control group consisted of 30 age- and sex-matched healthy non-smoker individuals. Venous blood was collected from the subjects for the evaluation of plasma levels of glutathione peroxidase, vitamin C, selenium, bilirubin, and uric acid. Results: Age mean and standard deviation of the patients (40.83, 12.74 was comparable to the controls (41.96, 13.08. Mean level of uric acid was significantly lower in PV patients compared to the controls (P = 0.006. Other antioxidants were not different between the two groups. Uric acid of the patients with mucosal involvement was significantly lower than patients with mucocutaneous involvement (P = 0.049. Limitations: The blood level of other antioxidants (e.g. malondialdehyde was not evaluated. Conclusions: Uric acid as an antioxidant in our study had similar changes to previous studies in the field of other diseases but selenium, bilirubin, and glutathione peroxidase did not differ between patients and controls.

  6. Clonal propagation of Bambusa vulgaris by leafy branch cuttings

    Institute of Scientific and Technical Information of China (English)

    M.S.Islam; M.K.Bhuiyan; M.M.Hossain; M.A.Hossain

    2011-01-01

    Bambusa vulgaris Schrad ex wendl is a widely cultivated bamboo species in rural Bangladesh for its versatile uses. The vegetative propagation becomes the only viable alternative for this species because B. vulgaris does not set seed after sparse flowering, which makes seed- ling progenies unavailable. A low-cost propagation trial was conducted to explore the clonal propagation techniques for the species with two types of small branch cuttings, nodal leafy cuttings and tip cuttings. The cuttings, after treating with 0, 0.1%, 0.4%, and 0.8% IBA solutions, were kept in non-mist propagator to let them to root for assessing the rooting ability. The cuttings were rooted in four weeks and were allowed to grow in the polybags for 10 months under nursery condition to assess their steckling capacity. The study reveals that both types of branch cuttings are able to develop roots, shoots, to survive and to form rhizome under the nursery condition. Rooting ability of the cuttings was significantly enhanced by the application of rooting hormone - IBA. The highest root- ing percentage in nodal leafy cuttings and the tip cuttings (56.67% and 51.0%, respectively) were observed in 0.8% IBA treatment, followed by 0.4% IBA and the lowest (34.3% and 30.0%, respectively) was in control. The highest number of root developed per cutting (9.77 and 8.33 in nodal leafy cuttings and the tip cuttings, respectively) was also obtained from the cuttings treated with 0.8% IBA solution, followed 0.4% IBA treat- ment and the lowest (3.1 and 2.1, respectively) was in the cuttings with- out treatment. However, the length of the longest root varied significantly neither with the cutting types nor the concentrations of IBA solution. Survival percentage of the stecklings in nursery condition was signifi- cantly enhanced by IBA.

  7. Transcriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem.

    Science.gov (United States)

    Padder, Bilal A; Kamfwa, Kelvin; Awale, Halima E; Kelly, James D

    2016-01-01

    Bean (Phaseolus vulgaris) anthracnose caused by the hemi-biotrophic pathogen Colletotrichum lindemuthianum is a major factor limiting production worldwide. Although sources of resistance have been identified and characterized, the early molecular events in the host-pathogen interface have not been investigated. In the current study, we conducted a comprehensive transcriptome analysis using Illumina sequencing of two near isogenic lines (NILs) differing for the presence of the Co-1 gene on chromosome Pv01 during a time course following infection with race 73 of C. lindemuthianum. From this, we identified 3,250 significantly differentially expressed genes (DEGs) within and between the NILs over the time course of infection. During the biotrophic phase the majority of DEGs were up regulated in the susceptible NIL, whereas more DEGs were up-regulated in the resistant NIL during the necrotrophic phase. Various defense related genes, such as those encoding PR proteins, peroxidases, lipoxygenases were up regulated in the resistant NIL. Conversely, genes encoding sugar transporters were up-regulated in the susceptible NIL during the later stages of infection. Additionally, numerous transcription factors (TFs) and candidate genes within the vicinity of the Co-1 locus were differentially expressed, suggesting a global reprogramming of gene expression in and around the Co-1 locus. Through this analysis, we reduced the previous number of candidate genes reported at the Co-1 locus from eight to three. These results suggest the dynamic nature of P. vulgaris-C. lindemuthianum interaction at the transcriptomic level and reflect the role of both pathogen and effector triggered immunity on changes in plant gene expression.

  8. Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough.

    Energy Technology Data Exchange (ETDEWEB)

    Chhabra, S.R.; He, Q.; Huang, K.H.; Gaucher, S.P.; Alm, E.J.; He,Z.; Hadi, M.Z.; Hazen, T.C.; Wall, J.D.; Zhou, J.; Arkin, A.P.; Singh, A.K.

    2005-09-16

    Desulfovibrio vulgaris Hildenborough belongs to a class ofsulfate-reducing bacteria (SRB) and is found ubiquitously in nature.Given the importance of SRB-mediated reduction for bioremediation ofmetal ion contaminants, ongoing research on D. vulgaris has been in thedirection of elucidating regulatory mechanisms for this organism under avariety of stress conditions. This work presents a global view of thisorganism's response to elevated growth temperature using whole-celltranscriptomics and proteomics tools. Transcriptional response (1.7-foldchange or greater; Z>1.5) ranged from 1,135 genes at 15 min to 1,463genes at 120 min for a temperature up-shift of 13oC from a growthtemperature of 37oC for this organism and suggested both direct andindirect modes of heat sensing. Clusters of orthologous group categoriesthat were significantly affected included posttranslationalmodifications; protein turnover and chaperones (up-regulated); energyproduction and conversion (down-regulated), nucleotide transport,metabolism (down-regulated), and translation; ribosomal structure; andbiogenesis (down-regulated). Analysis of the genome sequence revealed thepresence of features of both negative and positive regulation whichincluded the CIRCE element and promoter sequences corresponding to thealternate sigma factors ?32 and ?54. While mechanisms of heat shockcontrol for some genes appeared to coincide with those established forEscherichia coli and Bacillus subtilis, the presence of unique controlschemes for several other genes was also evident. Analysis of proteinexpression levels using differential in-gel electrophoresis suggestedgood agreement with transcriptional profiles of several heat shockproteins, including DnaK (DVU0811), HtpG (DVU2643), HtrA (DVU1468), andAhpC (DVU2247). The proteomics study also suggested the possibility ofposttranslational modifications in the chaperones DnaK, AhpC, GroES(DVU1977), and GroEL (DVU1976) and also several periplasmic ABCtransporters.

  9. Energy metabolism in Desulfovibrio vulgaris Hildenborough: insights from transcriptome analysis

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Patricia M.; He, Qiang; Valente, Filipa M.A.; Xavier, Antonio V.; Zhou, Jizhong; Pereira, Ines A.C.; Louro, Ricardo O.

    2007-11-01

    Sulphate-reducing bacteria are important players in the global sulphur and carbon cycles, with considerable economical and ecological impact. However, the process of sulphate respiration is still incompletely understood. Several mechanisms of energy conservation have been proposed, but it is unclear how the different strategies contribute to the overall process. In order to obtain a deeper insight into the energy metabolism of sulphate-reducers whole-genome microarrays were used to compare the transcriptional response of Desulfovibrio vulgaris Hildenborough grown with hydrogen/sulphate, pyruvate/sulphate, pyruvate with limiting sulphate, and lactate/thiosulphate, relative to growth in lactate/sulphate. Growth with hydrogen/sulphate showed the largest number of differentially expressed genes and the largest changes in transcript levels. In this condition the most up-regulated energy metabolism genes were those coding for the periplasmic [NiFeSe]hydrogenase, followed by the Ech hydrogenase. The results also provide evidence for the involvement of formate cycling and the recently proposed ethanol pathway during growth in hydrogen. The pathway involving CO cycling is relevant during growth on lactate and pyruvate, but not during growth in hydrogen as the most down-regulated genes were those coding for the CO-induced hydrogenase. Growth on lactate/thiosulphate reveals a down-regulation of several energymetabolism genes similar to what was observed in the presence of nitrite. This study identifies the role of several proteins involved in the energy metabolism of D. vulgaris and highlights several novel genes related to this process, revealing a more complex bioenergetic metabolism than previously considered.

  10. Enzymatic and non-enzymatic antioxidant potentials of Chlorella vulgaris grown in effluent of a confectionery industry

    OpenAIRE

    2011-01-01

    Enzymatic and non-enzymatic antioxidant potentials of Chlorella vulgaris have gained considerable importance in recent decades. C. vulgaris strain highly tolerant to extreme pH variations was isolated and mass-cultivated in the wastewater from a confectionery industry. C.vulgaris showed better growth in wastewater than in improvised CFTRI medium. The microalgal biomass was then screened for the following antioxidants: peroxidase, superoxide dismutase, polyphenol oxidase, glutathione peroxidas...

  11. Hubungan Stres dengan Kejadian Akne Vulgaris Di kalangan Mahasiswa Fakultas Kedokteran Universitas Sumatera Utara Angkatan 2007-2009

    OpenAIRE

    Perumal, Nitya

    2011-01-01

    Background: Acne or acne vulgaris has always been well concerned among teenagers and adolescent age. Acne vulgaris is a common disorder of the follicle sebaceous unit that predominantly occurs in adolescence. Acne vulgaris which is suffered by 95 to 100 percent boys and 83 to 85 percent girls. This skin disease is caused by multifactoral of skin disease, which is stress. With the increased studying burden, packed time table and inadequate sleep has caused medical students in University of Nor...

  12. Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury

    National Research Council Canada - National Science Library

    Grespan, Renata; Aguiar, Rafael Pazinatto; Giubilei, Frederico Nunes; Fuso, Rafael Rocco; Damião, Marcio José; Silva, Expedito Leite; Mikcha, Jane Graton; Hernandes, Luzmarina; Bersani Amado, Ciomar; Cuman, Roberto Kenji Nakamura

    2014-01-01

    .... This study investigated the hepatoprotective effect of Thymus vulgaris essential oil (TEO), which is used popularly for various beneficial effects, such as its antiseptic, carminative, and antimicrobial effects...

  13. Uranium and cesium accumulation in bean (Phaseolus vulgaris L. var. vulgaris) and its potential for uranium rhizofiltration.

    Science.gov (United States)

    Yang, Minjune; Jawitz, James W; Lee, Minhee

    2015-02-01

    Laboratory scale rhizofiltration experiments were performed to investigate uranium and cesium accumulation in bean (Phaseolus vulgaris L. var. vulgaris) and its potential for treatment of uranium contaminated groundwater. During 72 h of rhizofiltration, the roots of the bean accumulated uranium and cesium to concentrations 317-1019 times above the initial concentrations, which ranged from 100 to 700 μg l(-1) in artificially contaminated solutions. When the pH of the solution was adjusted to 3, the ability to accumulate uranium was 1.6 times higher than it was for solutions of pH 7 and pH 9. With an initial uranium concentration of 240 μg l(-1) in genuine groundwater at pH 5, the bean reduced the uranium concentration by 90.2% (to 23.6 μg l(-1)) within 12 h and by 98.9% (to 2.8 μg l(-1)) within 72 h. A laboratory scale continuous clean-up system reduced uranium concentrations from 240 μg l(-1) to below 10 μg l(-1) in 56 h; the whole uranium concentration in the bean roots during system operation was more than 2600 μg g(-1) on a dry weight basis. Using SEM and EDS analyses, the uranium removal in solution at pH 7 was determined based on adsorption and precipitation on the root surface in the form of insoluble uranium compounds. The present results demonstrate that the rhizofiltration technique using beans efficiently removes uranium and cesium from groundwater as an eco-friendly and cost-effective method.

  14. Effect of starvation stress on morphological changes and production of adhesive exopolysaccharide (EPS by Proteus vulgaris

    Directory of Open Access Journals (Sweden)

    Kamila Myszka

    2011-09-01

    Full Text Available   Background. Proteus vulgaris attach to available surfaces in industrial environments, can develop into extensive biofilm. Such bacterial layer is a potential source of contamination of foods that may lead to spoilage or transmission foodborne pathogens. The purpose of these investigations was to evaluate the influence of limited nutrients availability in the medium on the morphological changes and biosynthesis of bacterial surface-associated EPS by P. vulgaris. The relationship between the dimension of cells, EPS production and P. vulgaris biofilm development process on stainless steel surfaces (type 316L was also examined. Material and methods. P. vulgaris ATCC 6380 was used in this study. The cultures were incubated at 37°C on the Enterobacteriaceae enrichment broth according to Mossel [1962]. During the investigations the medium with optimal and 10 times diluted optimal of nutrient availability were used. For cells dimension analysis a Carl-Zeiss Axiovert 200 inverted microscope and a scanning electron microscope (LEO 435VP was applied. Isolation of exopolysaccharides was based on the procedure employed by Forde and Fitzgerald [1999]. To determine the level of P. vulgaris adhesion to the surface of stainless steel, the method described by Le Thi et al. [2001] was used. Results. In all experimental variants the area of P. vulgaris cells was changed upon long-term starvation. Altering of physical dimension of bacteria was effected by the decreasing value of the cell length. The change of P. vulgaris morphology promoted the beginning stages of biofilm formation process on the surface of stainless steel. Under starvation conditions P. vulgaris produced more EPS. It was observed with an increase of incubation period. These extracellular molecules initiated more advanced stages of P. vulgaris biofilm formation on examined surfaces. Conclusion. The data support the notion thatcellular factors influencing P. vulgaris adhesion process to abiotic

  15. XML Investigating the Phytochemical, Antibacterial and Antifungal Effects of Thymus Vulgaris and Cuminum Cyminum Essential Oils

    Directory of Open Access Journals (Sweden)

    Soghra Valizadeh (MSc

    2016-02-01

    Full Text Available Background and Objective: The aim of this study was to determine the chemical composition, antibacterial and antifungal effects of Thymus vulgaris and Cuminum Cyminum essential oils against foodborne pathogens and Candida species in vitro. Methods: The essential oils were extracted from the aerial parts of Thymus vulgaris and dried Cuminum Cyminum seeds using a Clevenger apparatus for 3 hours. Analysis of the essential oils’ constituents was performed using gas chromatography-mass spectrophotometry. The antibacterial activity of Cuminum Cyminum essential oil and essential oil of Thymus vulgaris against Bacillus cereus, Listeria monocytogenes, Escherichia coli and Salmonella typhimurium were evaluated in agar culture medium. The minimum inhibitory concentration (MIC of these essential oils against fungal strains of Candida albicans, C. tropicalis, C. parapsilosis and C. dubliniensis was measured. Results: Thymol (64.45% and cuminaldehyde (29.02% were the main components of the essential oil of Thymus vulgaris and Cuminum Cyminum, respectively. The largest inhibition zone diameter in the essential oils of Thymus vulgaris and Cuminum Cyminum in the agar disk diffusion method was related to B. cereus with 30 and 21 mm diameter, respectively. The largest growth inhibition zone diameter by the essential oil of Thymus vulgaris in the well diffusion method was 21 mm and against B. cereus. The MIC of essential oil of Thymus vulgaris in the microdilution method was 0.09% against all the four Candida strains. The MIC of Cuminum Cyminum essential oil against strains of C. albicans and C. tropicalis was 0.39%, while it was found as 0.19% against C. parapsilosis and C. dubliniensis. Conclusion: In this study, Cuminum Cyminum essential oil and essential oil of Thymus vulgaris show suitable inhibitory effects against the growth of bacteria using well and disk diffusion methods. Regarding the antifungal effects, the MIC of essential oil of Thymus vulgaris is

  16. A meta-analysis of association between acne vulgaris and Demodex infestation

    Institute of Scientific and Technical Information of China (English)

    Ya-e ZHAO; Li HU; Li-ping WU; Jun-xian MA

    2012-01-01

    Until now,etiology of acne vulgaris is still uncertain.Although clinicians usually deny the association between Demodex infestation and acne vulgaris,it has been proved in some clinical practices.To confirm the association between Demodex infestation and acne vulgaris,a meta-analysis was conducted.Predefined selection criteria were applied to search all published papers that analyzed the association between Demodex infestation and acne vulgaris (January 1950 to August 2011) in ISl Web of Knowledge,MEDLINE,and China National Knowledge Infrastructure (CNKI) databases.A meta-analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs)based on fixed effects models or random effects models.We enrolled the 60 Chinese and 3 English papers in this meta-analysis,which covered Turkey and 25 different provinces/municipalities in China and 42130 participants including students and residents,aged from 1 to 78 years.The pooled OR in random effects models is 2.80 (95% Cl,2.34-3.36).Stability is robust according to sensitivity analysis.The fail-safe number is 18477,suggesting that at least 18477 articles with negative conclusions would be needed to reverse the conclusion that acne vulgaris was related to Demodex infestation.So the effect of publication bias was insignificant and could be ignored.It was concluded that acne vulgaris is associated with Demodex infestation.This indicates that when regular treatments for acne vulgaris are ineffective,examination of Demodex mites and necessary acaricidal therapies should be considered.

  17. The culture of Chlorella vulgaris with human urine in multibiological life support system experiments

    Science.gov (United States)

    Li, Ming; Liu, Hong; Tong, Ling; Fu, Yuming; He, Wenting; Hu, Enzhu; Hu, Dawei

    The Integrative Experimental System (IES) was established as a tool to evaluate the rela-tionship of the subsystems in Bioregenerative Life Support System, and Multibiological Life Support System Experiments (MLSSE) have been conducted in the IES. The IES consists of a higher plant chamber, an animal chamber and a plate photo bioreactor (PPB) which cultivated lettuce (Lactuca sativa L.), silkworm (Bombyx Mori L.) and microalgae (Chlorella vulgaris), respectively. In MLSSE, four volunteers took turns breathing the system air through a tube connected with the animal chamber periodically. According to the CO2 concentration in the IES, the automotive control system of the PPB changed the light intensity regulating the photosynthesis of Chlorella vulgaris to make CO2 /O2 in the system maintain at stable levels. Chlorella vulgaris grew with human urine by carrying certain amount of alga liquid out of the bioreactor every day with synthetic urine replenished into the system, and O2 was regenerated, at the same time human urine was purified. Results showed that this IES worked stably and Chlorella vulgaris grew well; The culture of Chlorella vulgaris could be used to keep the balance of CO2 and O2 , and the change of light intensity could control the gas composition in the IES; Microalgae culture could be used in emergency in the system, the culture of Chlorella vulgaris could recover to original state in 5 days; 15.6 ml of condensation water was obtained every day by the culture of Chlorella vulgaris; The removal efficiencies of N, P in human urine could reach to 98.2% and 99.5%.

  18. Decreased eicosapentaenoic acid levels in acne vulgaris reveals the presence of a proinflammatory state.

    Science.gov (United States)

    Aslan, İbrahim; Özcan, Filiz; Karaarslan, Taner; Kıraç, Ebru; Aslan, Mutay

    2017-01-01

    This study aimed to determine circulating levels of polyunsaturated fatty acids (PUFAs), secretory phospholipase A2 (sPLA2), lipoprotein lipase (LPL) and measure circulating protein levels of angiopoietin-like protein 3 (ANGPTL3), ANGPTL4, cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) in patients with acne vulgaris. Serum from 21 control subjects and 31 acne vulgaris patients were evaluated for levels of arachidonic acid (AA, C20:4n- 6), dihomo-gamma-linolenic acid (DGLA, C20:3n-6), eicosapentaenoic acid (EPA, C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3). PUFA levels were determined by an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Lipid profile, routine biochemical and hormone parameters were assayed by standard kit methods Serum EPA levels were significantly decreased while AA/EPA and DGLA/EPA ratio were significantly increased in acne vulgaris patients compared to controls. Serum levels of AA, DGLA and DHA showed no significant difference while activity of sPLA2 and LPL were significantly increased in acne vulgaris compared to controls. Results of this study reveal the presence of a proinflammatory state in acne vulgaris as shown by significantly decreased serum EPA levels and increased activity of sPLA2, AA/EPA and DGLA/EPA ratio. Increased LPL activity in the serum of acne vulgaris patients can be protective through its anti-dyslipidemic actions. This is the first study reporting altered EPA levels and increased sPLA2 activity in acne vulgaris and supports the use of omega-3 fatty acids as adjuvant treatment for acne patients.

  19. Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp

    Directory of Open Access Journals (Sweden)

    Mohd Sajjad Ahmad Khan

    2014-06-01

    Full Text Available Emergence of drug-resistant strains has demanded for alternative means of combating fungal infections. Oils of Carum copticum and Thymus vulgaris have long been used in ethnomedicine for ailments of various fungal infections. Since their activity has not been reported in particular against drug-resistant fungi, this study was aimed to evaluate the effects of oils of C. copticum and T. vulgaris on the growth and virulence of drug-resistant strains of Aspergillus spp. and Trichophyton rubrum. The gas chromatography-mass spectrometry analysis revealed thymol constituting 44.71% and 22.82% of T. vulgaris and C. copticum, respectively. Inhibition of mycelial growth by essential oils was recorded in the order of thymol > T. vulgaris > C. copticum against the tested strains. RBC lysis assay showed no tested oils to be toxic even up to concentration two folds higher than their respective MFCs. Thymol exhibited highest synergy in combination with fluconazole against Aspergillus fumigatus MTCC2550 (FICI value 0.187 and T. rubrum IOA9 (0.156 as determined by checkerboard method. Thymol and T. vulgaris essential oil were equally effective against both the macro and arthroconidia growth (MIC 72 µg/mL. A > 80% reduction in elastase activity was recorded for A. fumigatus MTCC2550 by C. copticum, T. vulgaris oils and thymol. The effectiveness of these oils against arthroconidia and synergistic interaction of thymol and T. vulgaris with fluconazole can be exploited to potentiate the antifungal effects of fluconazole against drug-resistant strains of T. rubrum and Aspergillus spp.

  20. Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp.

    Science.gov (United States)

    Khan, Mohd Sajjad Ahmad; Ahmad, Iqbal; Cameotra, Swaranjit Singh

    2014-01-01

    Emergence of drug-resistant strains has demanded for alternative means of combating fungal infections. Oils of Carum copticum and Thymus vulgaris have long been used in ethnomedicine for ailments of various fungal infections. Since their activity has not been reported in particular against drug-resistant fungi, this study was aimed to evaluate the effects of oils of C. copticum and T. vulgaris on the growth and virulence of drug-resistant strains of Aspergillus spp. and Trichophyton rubrum. The gas chromatography-mass spectrometry analysis revealed thymol constituting 44.71% and 22.82% of T. vulgaris and C. copticum, respectively. Inhibition of mycelial growth by essential oils was recorded in the order of thymol > T. vulgaris > C. copticum against the tested strains. RBC lysis assay showed no tested oils to be toxic even up to concentration two folds higher than their respective MFCs. Thymol exhibited highest synergy in combination with fluconazole against Aspergillus fumigatus MTCC2550 (FICI value 0.187) and T. rubrum IOA9 (0.156) as determined by checkerboard method. Thymol and T. vulgaris essential oil were equally effective against both the macro and arthroconidia growth (MIC 72 μg/mL). A > 80% reduction in elastase activity was recorded for A. fumigatus MTCC2550 by C. copticum, T. vulgaris oils and thymol. The effectiveness of these oils against arthroconidia and synergistic interaction of thymol and T. vulgaris with fluconazole can be exploited to potentiate the antifungal effects of fluconazole against drug-resistant strains of T. rubrum and Aspergillus spp.

  1. Global analysis of heat shock response in Desulfovibrio vulgaris Hildenborough.

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    Arkin, A. P. (Physical Biosciences Division, Berkeley, CA); Wall, J. D. (University of Missouri-Columbia, Columbia, MO); Hazen, T. C. (Physical Biosciences Division, Berkeley, CA); He, Z. (Oak Ridge National Laboratory, Oak Ridge, TN); Zhou, J. (Oak Ridge National Laboratory, Oak Ridge, TN); Huang, K. H. (Physical Biosciences Division, Berkeley, CA); Gaucher, Sara P.; He, Q. (Oak Ridge National Laboratory, Oak Ridge, TN); Hadi, Masood Z.; Chhabra, Swapnil R.; Alm, Eric J. (Physical Biosciences Division, Berkeley, CA); Singh, A. K.

    2005-08-01

    Desulfovibrio vulgaris Hildenborough belongs to a class of sulfate-reducing bacteria (SRB) and is found ubiquitously in nature. Given the importance of SRB-mediated reduction for bioremediation of metal ion contaminants, ongoing research on D. vulgaris has been in the direction of elucidating regulatory mechanisms for this organism under a variety of stress conditions. This work presents a global view of this organism's response to elevated growth temperature using whole-cell transcriptomics and proteomics tools. Transcriptional response (1.7-fold change or greater; Z {ge} 1.5) ranged from 1,135 genes at 15 min to 1,463 genes at 120 min for a temperature up-shift of 13 C from a growth temperature of 37 C for this organism and suggested both direct and indirect modes of heat sensing. Clusters of orthologous group categories that were significantly affected included posttranslational modifications; protein turnover and chaperones (up-regulated); energy production and conversion (down-regulated), nucleotide transport, metabolism (down-regulated), and translation; ribosomal structure; and biogenesis (down-regulated). Analysis of the genome sequence revealed the presence of features of both negative and positive regulation which included the CIRCE element and promoter sequences corresponding to the alternate sigma factors {sigma}{sup 32} and {sigma}{sup 54}. While mechanisms of heat shock control for some genes appeared to coincide with those established for Escherichia coli and Bacillus subtilis, the presence of unique control schemes for several other genes was also evident. Analysis of protein expression levels using differential in-gel electrophoresis suggested good agreement with transcriptional profiles of several heat shock proteins, including DnaK (DVU0811), HtpG (DVU2643), HtrA (DVU1468), and AhpC (DVU2247). The proteomics study also suggested the possibility of posttranslational modifications in the chaperones DnaK, AhpC, GroES (DVU1977), and GroEL (DVU

  2. Inhibition of lentivirus replication by aqueous extracts of Prunella vulgaris

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    Hauck Cathy

    2009-01-01

    Full Text Available Abstract Background Various members of the mint family have been used historically in Chinese and Native American medicine. Many of these same family members, including Prunella vulgaris, have been reported to have anti-viral activities. To further characterize the anti-lentiviral activities of P. vulgaris, water and ethanol extractions were tested for their ability to inhibit equine infectious anemia virus (EIAV replication. Results Aqueous extracts contained more anti-viral activity than did ethanol extracts, displaying potent anti-lentiviral activity against virus in cell lines as well as in primary cell cultures with little to no cellular cytotoxicity. Time-of-addition studies demonstrated that the extracts were effective when added during the first four h of the viral life cycle, suggesting that the botanical constituents were targeting the virion itself or early entry events. Further analysis revealed that the extracts did not destroy EIAV virion integrity, but prevented viral particles from binding to the surface of permissive cells. Modest levels of anti-EIAV activity were also detected when the cells were treated with the extracts prior to infection, indicating that anti-EIAV botanical constituents could interact with both viral particles and permissive cells to interfere with infectivity. Size fractionation of the extract demonstrated that eight of the nine fractions generated from aqueous extracts displayed anti-viral activity. Separation of ethanol soluble and insoluble compounds in the eight active fractions revealed that ethanol-soluble constituents were responsible for the anti-viral activity in one fraction whereas ethanol-insoluble constituents were important for the anti-viral activity in two of the other fractions. In three of the five fractions that lost activity upon sub-fractionation, anti-viral activity was restored upon reconstitution of the fractions, indicating that synergistic anti-viral activity is present in several

  3. Protective Effect of Zizphus Vulgaris Extract, on Liver Toxicity in Laboratory Rats

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    S Ebrahimi

    2011-06-01

    Full Text Available Introduction & Objective: Some of natural and synthetic products have antioxidant properties which protect the liver against the destructive factors. This study aimed to investigate the effect of Zizphus Vulgaris extracts on mice liver. Materials & Methods: This experimental study was conducted at Yasouj University of Medical Sciences in 2010 on 30 healthy adult male Wistar rats. Animals were randomly divided into five equal groups: the control group (receiving, olive oil, control group (receiving olive oil and carbon tetrachloride and three intervention groups (receiving different dose of carbon tetrachloride and olive oil groups. The intervention group was given daily doses of 200, 400 and 600 mg per Kg of Zizphus Vulgaris extract by gavage respectively. After 45 days, the amount of liver enzymes, total protein, albumin and bilirubin in animal’s sera were measured. Data were analyzed by the SPSS software, using ANOVA and t-test. Results: The concentration of total protein, albumin, AST, ALT, ALP in test groups I, II and III receiving Z.Vulgaris extract (200, 400 and 600 mg/kg weight compared with control group were statistically not significant. Consumption of Z.Vulgaris reduced the bilirubin concentration in test groups I and II but this decrease was significant only in the test group I Increasing of Z.Vulgaris dose in the test group III (600 mg Z.Vulgaris per kg body weight showed increase in the level of serum bilirubin. Increase in the ratio of liver weight to body weight of rats in groups I and III in comparison with control groups was noticed although this difference was not statistically significant. Findings of this study revealed that dosage of 600 mg/kg extract of Z.Vulgaris caused significant improvements in CCl4 induced liver necrosis (P <0.01 and reduced portal cells inflammation (P <0.01. Dose of 400 mg/kg of Z.Vulgaris induced some destruction and necrosis of liver cells in animals but significant reduction of portal cells

  4. Orally administered extract from Prunella vulgaris attenuates spontaneous colitis in mdr1a-/-mice

    Institute of Scientific and Technical Information of China (English)

    Kelley; MK; Haarberg; Meghan; J; Wymore; Brand; Anne-Marie; C; Overstreet; Catherine; C; Hauck; Patricia; A; Murphy; Jesse; M; Hostetter; Amanda; E; Ramer-Tait; Michael; J; Wannemuehler

    2015-01-01

    AIM: To investigate the ability of a Prunella vulgaris(P. vulgaris) ethanolic extract to attenuate spontaneous typhlocolitis in mdr1a-/- mice. METHODS: Vehicle(5% ethanol) or P. vulgaris ethanolic extract(2.4 mg/d) were administered daily by oral gavage to mdr1a-/- or wild type FVBWT mice from 6 wk of age up to 20 wk of age. Clinical signs of disease were noted by monitoring weight loss. Mice experiencingweight loss in excess of 15% were removed from the study. At the time mice were removed from the study, blood and colon tissue were collected for analyses that included histological evaluation of lesions, inflammatory cytokine levels, and myeloperoxidase activity. RESULTS: Administration of P. vulgaris extracts to mdr1a-/- mice delayed onset of colitis and reduced severity of mucosal inflammation when compared to vehicle-treated mdr1a-/- mice. Oral administration of the P. vulgaris extract resulted in reduced(P < 0.05) serum levels of IL-10(4.6 ± 2 vs 19.4 ± 4), CXCL9(1319.0 ± 277 vs 3901.0 ± 858), and TNFα(9.9 ± 3 vs 14.8 ± 1) as well as reduced gene expression by more than two-fold for Ccl2, Ccl20, Cxcl1, Cxcl9, IL-1 α, Mmp10, VCAM-1, ICAM, IL-2, and TNFα in the colonic mucosa of mdr1a-/- mice compared to vehicle-treated mdr1a-/-mice. Histologically, several microscopic parameters were reduced(P < 0.05) in P. vulgaris-treated mdr1a-/-mice, as was myeloperoxidase activity in the colon(2.49 ± 0.16 vs 3.36 ± 0.06, P < 0.05). The numbers of CD4+ T cells(2031.9 ± 412.1 vs 5054.5 ± 809.5) and germinal center B cells(2749.6 ± 473.7 vs 4934.0 ± 645.9) observed in the cecal tonsils of P. vulgaris-treated mdr1a-/- were significantly reduced(P < 0.05) from vehicle-treated mdr1a-/- mice. Vehicle-treated mdr1a-/- mice were found to produce serum antibodies to antigens derived from members of the intestinal microbiota, indicative of severe colitis and a loss of adaptive tolerance to the members of the microbiota. These serum antibodies were greatly reduced or

  5. Effects of magnetic field on the antioxidant defense system of recirculation-cultured Chlorella vulgaris.

    Science.gov (United States)

    Wang, Hai-Ying; Zeng, Xiao-Bo; Guo, Si-Yuan; Li, Zong-Tao

    2008-01-01

    Little is known about the influence of magnetic fields (MF) on growth of microalgae such as Chlorella vulgaris, which has been consumed as health food for various nutritional and pharmacological effects. This preliminary study investigated whether static MF can modulate the antioxidant system in C. vulgaris by exposing the cells to static MF generated by dual yoke electromagnets with magnetic flux density of 10-50 mT for 12 h. After exposure to 10-35 mT for 12 h, the activity of superoxide dismutases and peroxidase increased significantly compared to control cells. However, a remarkable increase of catalase activity occurred at 45 and 50 mT. The lipid peroxidation of algae cells determined by production of thiobarbituric acid-reactive substances was much increased when exposed to 35, 45, and 50 mT of MF. The scavenging ability of 2,2-diphenyl-1-picrylhydrazyl radical was decreased markedly while there was no variation of total carotenoids content in C. vulgaris cells. Assay of specific growth rate in 72 h cultivation after MF exposure was also conducted. In groups after exposure to 10-35 mT of MF, specific growth rate was significantly increased. These results suggest that 10-35 mT of static MF exposure could promote the growth of C. vulgaris and regulate its antioxidant defense system to protect cells efficiently, which could possibly enhance the growth of C. vulgaris in industrialized cultivation by MF.

  6. Magnesium Uptake by the Green Microalga Chlorella vulgaris in Batch Cultures.

    Science.gov (United States)

    Ben Amor-Ben Ayed, Hela; Taidi, Behnam; Ayadi, Habib; Pareau, Dominique; Stambouli, Moncef

    2016-03-01

    The accumulation (internal and superficial distribution) of magnesium ions (Mg(2+)) by the green freshwater microalga Chlorella vulgaris (C. vulgaris) was investigated under autotrophic culture in a stirred photobioreactor. The concentrations of the three forms of Mg(2+) (dissolved, extracellular, and intracellular) were determined with atomic absorption spectroscopy during the course of C. vulgaris growth. The proportions of adsorbed (extracellular) and absorbed (intracellular) Mg(2+) were quantified. The concentration of the most important pigment in algal cells, chlorophyll a, increased over time in proportion to the increase in the biomass concentration, indicating a constant chlorophyll/biomass ratio during the linear growth phase. The mean-average rate of Mg(2+) uptake by C. vulgaris grown in a culture medium starting with 16 mg/l of Mg(2+) concentration was measured. A clear relationship between the biomass concentration and the proportion of the Mg(2+) removal from the medium was observed. Of the total Mg(2+) present in the culture medium, 18% was adsorbed on the cell wall and 51% was absorbed by the biomass by the end of the experiment (765 h). Overall, 69% of the initial Mg(2+) were found to be removed from the medium. This study supported the kinetic model based on a reversible first-order reaction for Mg(2+) bioaccumulation in C. vulgaris, which was consistent with the experimental data.

  7. Mechanisms of Eu(III) and Cm(III) Association With Chlorella Vulgaris

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    Ozaki, T.; Kimura, T.; Ohnuki, T.; Francis, A. J.

    2002-12-01

    Association of Eu(III) and Cm(III) with Chlorella vulgaris and cellulose was studied by a batch method, time-resolved laser-induced fluorescence spectroscopy (TRLFS) and extended X-ray absorption fine structure (EXAFS). The kinetics study performed by a batch method showed that the maximum adsorption of Eu(III) and Cm(III) on C. vulgaris was attained within three minutes of contact time, and afterwards the percentage adsorption decreased with time due to exudates released from C. vulgaris with affinity for Eu(III) and Cm(III). TRLFS showed that the short-term adsorption of Eu(III) on C. vulgaris was attributed to their coordination with the cell wall components comprised of cellulose. TRLFS also demonstrated that Eu(III) coordinated with the functional groups of cellulose very weakly in spite of the large distribution coefficients observed. EXAFS analysis showed the local structure around the Eu(III) adsorbed on cellulose and with C. vulgaris was similar. These results indicate that the reactions both at cell surfaces through the adsorption as well as in solution phases through chelation with the exudates are important in estimating the environmental behavior of Eu(III) and Cm(III) in aqueous environments.

  8. Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris.

    Science.gov (United States)

    Jo, Beom-Ho; Lee, Chang Soo; Song, Hae-Ryong; Lee, Hyung-Gwan; Oh, Hee-Mock

    2014-09-01

    A strain-specific identification method is required to secure Chlorella strains with useful genetic traits, such as a fast growth rate or high lipid productivity, for application in biofuels, functional foods, and pharmaceuticals. Microsatellite markers based on simple sequence repeats can be a useful tool for this purpose. Therefore, this study developed five novel microsatellite markers (mChl-001, mChl-002, mChl-005, mChl-011, and mChl-012) using specific loci along the chloroplast genome of Chlorella vulgaris. The microsatellite markers were characterized based on their allelic diversities among nine strains of C. vulgaris with the same 18S rRNA sequence similarity. Each microsatellite marker exhibited 2~5 polymorphic allele types, and their combinations allowed discrimination between seven of the C. vulgaris strains. The two remaining strains were distinguished using one specific interspace region between the mChl-001 and mChl-005 loci, which was composed of about 27 single nucleotide polymorphisms, 13~15 specific sequence sites, and (T)n repeat sites. Thus, the polymorphic combination of the five microsatellite markers and one specific locus facilitated a clear distinction of C. vulgaris at the strain level, suggesting that the proposed microsatellite marker system can be useful for the accurate identification and classification of C. vulgaris.

  9. Quality of life in acne vulgaris: Relationship to clinical severity and demographic data

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    Aayush Gupta

    2016-01-01

    Full Text Available Background: Acne vulgaris is known to impair many aspects of quality of life. However, the correlation of this impairment with clinical severity remains equivocal despite various school, community and hospital-based studies. Aim: A hospital-based study was undertaken to measure the impairment of quality of life of patients of acne vulgaris and correlate it with the severity of lesions. Methods: This was a cross-sectional, questionnaire-based study in a cohort of 100 patients of acne vulgaris attending the outpatient department of our referral hospital. A physician measured the severity of lesions using the global acne grading system, and patients assessed quality of life by completing a questionnaire (Cardiff acne disability index. A correlation of these two was done; some additional correlations were brought out through demographic data collected from the patients. Results: There was no correlation between the severity of acne vulgaris and an impaired quality of life. Patients who consumed alcohol and/or smoked cigarettes were found to have an impaired quality of life. While the severity of acne progressively lessened in older patients, the impact on quality of life increased. Limitations: The sample size was small and there was a lack of guaranteed reliability on the self-reported quality of life. Conclusion: The severity of acne vulgaris does not correlate with impairment in quality of life.

  10. The use of sodium sulfacetamide 10%-sulfur 5% emollient foam in the treatment of acne vulgaris.

    Science.gov (United States)

    Del Rosso, James Q

    2009-08-01

    Acne vulgaris is the most common disorder encountered in ambulatory clinical practice comprising 11.3 percent of office visits to dermatologists in 2005.(1) By comparison, eczematous dermatoses, psoriasis, and skin cancer accounted for 6.2, 3.5, and 10 percent of office visits, respectively.(1) A variety of topical therapeutic options are available for treatment of acne vulgaris, including benzoyl peroxide, antibiotics, retinoids, azelaic acid, and sodium sulfacetamide-sulfur.(2,3) Sodium sulfacetamide 10%-sulfur 5% has been used for the topical treatment of seborrheic dermatitis, acne vulgaris, and rosacea since the mid-1950s and is available in a variety of formulations, including lotions, creams, cleansers, and emollient foams.(4) Recently, an emollient foam sodium sulfacetamide 10%-sulfur 5% formulation indicated for topical therapy of acne vulgaris, rosacea, and seborrheic dermatitis has become available.(5) This article provides an overview of the sodium sulfacetamide 10%-sulfur 5% emollient foam and reports the results of a case report series of patients with acne vulgaris treated with sodium sulfacetamide 10%-sulfur 5% emollient foam as monotherapy or in combination with other topical acne products.

  11. The Optimizing of Growth and Quality of Chlorella vulgaris as ASUH feed supplement for Broiler

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    Salvia Salvia

    2014-01-01

    Full Text Available Chlorella is spherical single celled freshwater micro-algae.   Chlorella  has potentials as element of ration or natural ASUH (safe, healthy, whole and halal feed supplement for it contains nutrition and active component, decreases cholesterol level and resulting darker yolk.  Chlorella vulgaris  is type of green algae   which, its economical potential need to be revealed. Variety of components of growing media is one of factors determining quality of microalgae. In terms of mass production, it is important to find correct, cheap and easy to feed nutrition for breeders. The objective of the research is to find out the optimizing of growth and quality of Chlorella vulgaris   as ASUH feed supplement for broiler. Test using sedgwick rafter method conducted to find out the optimizing of growth and quality of  Chlorella vulgaris  while AOAC method applied to test quality of its nutrition. The result shows that.  Chlorella vulgaris  grew well at technical medium 10 % of Phyto-s, crude protein 57.63%, fat  5.84%, b Carotene 6.44 mg/gram, Vitamin C 4.12 mg/gram and vitamin E 1.32 mg/gram. We can say that Chlorella vulgaris  potential to be natural and ASUH feed supplement and Phyto-s can be used as nutrition for mass production.

  12. Evaluating of Life Quality in Patients with Acne Vulgaris Using Generic and Specific Questionnaires

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    Reza Ghaderi

    2013-01-01

    Full Text Available Background. Acne vulgaris is a common skin disease that can adversely affect the quality of life of patients. Objective. The aim of this study was to determine the quality of life in patients with acne vulgaris. Methods. This study was carried out on 70 patients with acne vulgaris (28 males, 42 females. All the patients filled out two Persian versions of questionnaires: short form 36 (SF-36 and Dermatology Life Quality Index (DLQI. The obtained data were analyzed by using SPSS software (version 17. Results. The scores for physical functioning, social functioning, and bodily pain domains in patients were over 70%, but the scores for role physical, general health, vitality, role emotional, and mental health in patients were under 70%. Scores on the DLQI in patients with acne vulgaris ranged from 0 to 22 (mean ± SD, 8.18 ± 4.83. After comparing mean score of DLQI with respect to gender and age, it was found that the difference between the two groups was not statistically significant. Conclusion. Acne vulgaris has a significant effect on the quality of life. There was not any significant gender or age related difference in QOL.

  13. Efficacy of Essential Oils of Thymus vulgaris and Origanum vulgare on Echinococcus granulosus

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    P. E. Pensel

    2014-01-01

    Full Text Available The aim of the present work was to determine the in vitro effect of T. vulgaris and O. vulgare essential oils against E. granulosus protoscoleces and cysts. Essential oils were added to the medium resulting in thymol final concentrations of 10 μg/mL. The essential oils had a time-dependent effect provoking the complete loss of protoscolex viability after 72 days of postincubation. The results were confirmed at the ultrastructure level. Loss of infectivity in protoscoleces incubated with O. vulgare after 60 days was observed. On the other hand, the weight of cysts recorded in mice inoculated with T. vulgaris treated protoscoleces was significantly lower than that obtained in control group. Gamma-glutamyl-transpeptidase activity was readily detected in the culture supernatant of protoscoleces treated either with the essential oils or thymol. T. vulgaris and O. vulgare essential oils and thymol can induce cell apoptosis of protoscoleces after short incubation times. The efficacy of T. vulgaris and O. vulgare essential oils was also demonstrated in vitro on E. granulosus murine cysts. Our data suggest that essential oils of T. vulgaris and O. vulgare have anthelmintic effect against protoscoleces and cysts of E. granulosus.

  14. Decreased profilaggrin expression in ichthyosis vulgaris is a result of selectively impaired posttranscriptional control.

    Science.gov (United States)

    Nirunsuksiri, W; Presland, R B; Brumbaugh, S G; Dale, B A; Fleckman, P

    1995-01-13

    Ichthyosis vulgaris is an autosomal dominant disorder of keratinization characterized by mild hyperkeratosis and reduced or absent keratohyalin granules in the epidermis. Profilaggrin, a major component of keratohyalin granules, is reduced or absent from the skin of individuals with ichthyosis vulgaris. In this report, we have further characterized the molecular basis of low profilaggrin expression, which occurs in this disease. In situ hybridization revealed little profilaggrin mRNA in ichthyosis vulgaris-affected epidermis. In keratinocytes cultured from the epidermis of affected individuals, the abundance of profilaggrin was reduced to less than 10% of normal controls, while the mRNA level was decreased to 30-60% of controls. Expression of K1 and loricrin, other markers of epidermal differentiation, were not affected. Nuclear run-on assays indicated that the decrease in mRNA levels was not caused by aberrant transcription. Nucleotide sequencing of 5'-upstream, 3'-non-coding, and flanking regions of the profilaggrin gene from ichthyosis vulgaris-affected individuals revealed only minor changes, probably due to genetic polymorphisms. Our results indicate that defective profilaggrin expression in ichthyosis vulgaris is a result of selectively impaired posttranscriptional control.

  15. Efficacy of Essential Oils of Thymus vulgaris and Origanum vulgare on Echinococcus granulosus.

    Science.gov (United States)

    Pensel, P E; Maggiore, M A; Gende, L B; Eguaras, M J; Denegri, M G; Elissondo, M C

    2014-01-01

    The aim of the present work was to determine the in vitro effect of T. vulgaris and O. vulgare essential oils against E. granulosus protoscoleces and cysts. Essential oils were added to the medium resulting in thymol final concentrations of 10 μg/mL. The essential oils had a time-dependent effect provoking the complete loss of protoscolex viability after 72 days of postincubation. The results were confirmed at the ultrastructure level. Loss of infectivity in protoscoleces incubated with O. vulgare after 60 days was observed. On the other hand, the weight of cysts recorded in mice inoculated with T. vulgaris treated protoscoleces was significantly lower than that obtained in control group. Gamma-glutamyl-transpeptidase activity was readily detected in the culture supernatant of protoscoleces treated either with the essential oils or thymol. T. vulgaris and O. vulgare essential oils and thymol can induce cell apoptosis of protoscoleces after short incubation times. The efficacy of T. vulgaris and O. vulgare essential oils was also demonstrated in vitro on E. granulosus murine cysts. Our data suggest that essential oils of T. vulgaris and O. vulgare have anthelmintic effect against protoscoleces and cysts of E. granulosus.

  16. Global transcriptional, physiological and metabolite analyses of Desulfovibrio vulgaris Hildenborough responses to salt adaptation

    Energy Technology Data Exchange (ETDEWEB)

    He, Z.; Zhou, A.; Baidoo, E.; He, Q.; Joachimiak, M. P.; Benke, P.; Phan, R.; Mukhopadhyay, A.; Hemme, C.L.; Huang, K.; Alm, E.J.; Fields, M.W.; Wall, J.; Stahl, D.; Hazen, T.C.; Keasling, J.D.; Arkin, A.P.; Zhou, J.

    2009-12-01

    The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by physiological, global transcriptional, and metabolite analyses. The growth of D. vulgaris was inhibited by high levels of NaCl, and the growth inhibition could be relieved by the addition of exogenous amino acids (e.g., glutamate, alanine, tryptophan) or yeast extract. Salt adaptation induced the expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). Genes involved in carbon metabolism, cell motility, and phage structures were repressed. Comparison of transcriptomic profiles of D. vulgaris responses to salt adaptation with those of salt shock (short-term NaCl exposure) showed some similarity as well as a significant difference. Metabolite assays showed that glutamate and alanine were accumulated under salt adaptation, suggesting that they may be used as osmoprotectants in D. vulgaris. A conceptual model is proposed to link the observed results to currently available knowledge for further understanding the mechanisms of D. vulgaris adaptation to elevated NaCl.

  17. In vitro activity of heather [Calluna vulgaris (L.) Hull] extracts on selected urinary tract pathogens

    Science.gov (United States)

    Vučić, Dragana M.; Petković, Miroslav R.; Rodić-Grabovac, Branka B.; Stefanović, Olgica D.; Vasić, Sava M.; Čomić, Ljiljana R.

    2014-01-01

    Calluna vulgaris L. Hull (Ericaceae) has been used for treatment of urinary tract infections in traditional medicine. In this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The concentrations of total phenols were examined by using Folin-Ciocalteu reagent and ranged between 67.55 to 142.46 mg GAE/g. The concentrations of flavonoids in extracts were determined using spectrophotometric method with aluminum chloride and the values ranged from 42.11 to 63.68 mg RUE/g. The aqueous extract of C. vulgaris showed a significant antibacterial activity. The values of MIC were in the range from 2.5 mg/ml to 20 mg/ml for this extract. Proteus vulgaris strains were found to be the most sensitive. The results obtained suggest that all tested extracts of C. vulgaris inhibit the growth of human pathogens, especially the aqueous extract. PMID:25428676

  18. Polymorphism of the catechol-O-methyltransferase gene in Han Chinese patients with psoriasis vulgaris

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    Lin Gao

    2009-01-01

    Full Text Available Psoriasis vulgaris is defined by a series of linked cellular changes in the skin: hyperplasia of epidermal keratinocytes, vascular hyperplasia and ectasia, and infiltration of T lymphocytes, neutrophils and other types of leukocytes in the affected skin. Catechol-O-methyltransferase ( COMT 158 polymorphism can reduce the activity of the COMT enzyme that may trigger defective differentiation of keratinocytes in psoriasis. Immunocytes can degrade and inactivate catecholamines via monamine oxidase (MAO and COMT in the cells. We hypothesized that the COMT-158 G > A polymorphism was associated with the risk of psoriasis vulgaris in Han Chinese people. In a hospital-based case-control study, 524 patients with psoriasis vulgaris and 549 psoriasis-free controls were studied. COMT-158 G > A polymorphism was genotyped using the PCR sequence-specific primer (PCR-SSP technique. We found no statistically significant association between the COMT-158 allele A and the risk of psoriasis vulgaris (p = 0.739 adjusted OR = 1.03; 95% CI = 0.81-1.31. This suggests that the COMT-158 G > A polymorphism may not contribute to the etiology of psoriasis vulgaris in the Han Chinese population.

  19. Efficacy of Essential Oils of Thymus vulgaris and Origanum vulgare on Echinococcus granulosus

    Science.gov (United States)

    Pensel, P. E.; Maggiore, M. A.; Gende, L. B.; Eguaras, M. J.; Denegri, M. G.; Elissondo, M. C.

    2014-01-01

    The aim of the present work was to determine the in vitro effect of T. vulgaris and O. vulgare essential oils against E. granulosus protoscoleces and cysts. Essential oils were added to the medium resulting in thymol final concentrations of 10 μg/mL. The essential oils had a time-dependent effect provoking the complete loss of protoscolex viability after 72 days of postincubation. The results were confirmed at the ultrastructure level. Loss of infectivity in protoscoleces incubated with O. vulgare after 60 days was observed. On the other hand, the weight of cysts recorded in mice inoculated with T. vulgaris treated protoscoleces was significantly lower than that obtained in control group. Gamma-glutamyl-transpeptidase activity was readily detected in the culture supernatant of protoscoleces treated either with the essential oils or thymol. T. vulgaris and O. vulgare essential oils and thymol can induce cell apoptosis of protoscoleces after short incubation times. The efficacy of T. vulgaris and O. vulgare essential oils was also demonstrated in vitro on E. granulosus murine cysts. Our data suggest that essential oils of T. vulgaris and O. vulgare have anthelmintic effect against protoscoleces and cysts of E. granulosus. PMID:25180033

  20. Epidemiology of pemphigus vulgaris in the Northeast China: a 10-year retrospective study.

    Science.gov (United States)

    Zhu, Xiaoling; Pan, Jisheng; Yu, Zhiming; Wang, Yanhua; Cai, Limin; Zheng, Shuyun

    2014-01-01

    The aim of the present study was to evaluate the incidence, epidemiological, clinical and therapeutic features of patients with pemphigus vulgaris in China. We retrospectively investigated a total of 221 patients with pemphigus vulgaris. The parameters including age at diagnosis/onset, sex, diagnostic methods, hospitalizations, side-effects with different treatments, pemphigus vulgaris-associated diseases, the therapeutic features and duration of follow up were evaluated. The male : female ratio was 1:1.40. The incidence rate was obviously higher in two age groups, 31-40 years and 41-50 years. The mean age at onset was 44.19 ± 21.45 years. The majority of patients (56.56%) presented the mucocutaneous type. In addition, we found that various doses of corticosteroids (including the mean initial dose, maximum control dose, total dose before reducing and hospitalized total dose) exhibited statistical differences between only corticosteroids and corticosteroids-immunosuppressant groups in mild, moderate and severe pemphigus vulgaris (P pemphigus vulgaris most frequently occurred in the 41-50-year age group in China and the majority of patients manifested mucocutaneous lesions. Importantly, the incidence rate in females was higher than in males. The patients who needed combined immunosuppressant treatment, usually needed higher doses of corticosteroids initially, to control the disease and over the total course of treatment.

  1. The use of biochip immunofluorescence microscopy for the diagnosis of Pemphigus vulgaris.

    Science.gov (United States)

    Russo, Irene; Saponeri, Andrea; Peserico, Andrea; Alaibac, Mauro

    2014-06-01

    Pemphigus vulgaris is an autoimmune intraepithelial blistering skin disease characterized by the presence of circulating autoantibodies directed against surfaces of keratinocytes. Diagnosis is generally based on clinical features, histology, direct and indirect immunofluorescence and ELISA. This study describes a new BIOCHIP mosaic-based indirect immunofluorescence technique based on recombinant antigenic substrates and transfected cells. We investigated the diagnostic use of BIOCHIP for the serological diagnosis of Pemphigus vulgaris. Autoantibodies against desmoglein 3 were detected in 97.62% of patients (41/42) with P. vulgaris. There were no positive results in the negative control group. Our study revealed that BIOCHIP has high sensitivity and specificity comparable to that of the ELISA assays. Therefore the BIOCHIP technique seems to be an appropriate method for the diagnosis of P. vulgaris as it has been shown to be a simple, standardized and readily available novel tool, which could facilitate the diagnosis of this autoimmune bullous disease. We suggest that it could be used as an initial screening test to identify patients with P. vulgaris before using the ELISA approach.

  2. Association of HSD17B3 and HSD3B1 polymorphisms with acne vulgaris in Southwestern Han Chinese.

    Science.gov (United States)

    Yang, Xiao-Yan; Wu, Wen-Juan; Yang, Cheng; Yang, Ting; He, Jun-Dong; Yang, Zhi; He, Li

    2013-01-01

    Acne vulgaris is a very common skin disorder. Previous studies have indicated that genetic background factors play key roles in the onset of acne. Our previous investigation implicated several genes in the androgen metabolism pathway with acne vulgaris in the Han Chinese population. Thus, we further investigated genes and genetic variants that play important roles in this pathway for their relationship with the pathology of acne. In this study, a total of 610 subjects, including 403 acne patients and 207 healthy controls, were genotyped for 15 single-nucleotide polymorphisms in HSD3B1 and HSD17B3 genes. This study shows that rs6428829 in HSD3B1 was associated with acne vulgaris in Han patients from Southwest China, even after adjusting for age and sex. The GG genotype was associated with an increased risk of acne vulgaris (p acne vulgaris (p acne vulgaris in the case-control study (p acne vulgaris. Based on these analyses, our study indicates that the cutaneous androgen metabolism-regulated genes HSD3B1 and HSD17B3 increase the susceptibility to acne vulgaris in Han Chinese from Southwest China.

  3. A study on aromatic profiles of Thymus hyemalis and Spanish T. vulgaris essential oils at five physiological stages

    Science.gov (United States)

    Shrubs of clonal selections of Thymus hyemalis L. and Spanish T. vulgaris subsp. vulgaris were harvested at five phenological stages during the plant growing cycle: vegetative (VEG), floral (FL), floral-fructification (FL-FR), fructification (FR), and passed fructification (FR-pas). The volatile pro...

  4. The inhibitory effect of Thymus vulgaris extracts on the planktonic form and biofilm structures of six human pathogenic bacteria

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    Zinab Mohsenipour

    2015-06-01

    Full Text Available Objective: Microorganisms are responsible for many problems in industry and medicine because of biofilm formation. Therefore, this study was aimed to examine the effect of Thymus vulgaris (T. vulgaris extracts on the planktonic form and biofilm structures of six pathogenic bacteria. Materials and methods: Antimicrobial activities of the plant extracts against the planktonic form of the bacteria were determined using the disc diffusion method. MIC and MBC values were evaluated using macrobroth dilution technique. Anti-biofilm effects were assessed by microtiter plate method. Results: According to disc diffusion test (MIC and MBC, the ability of Thymus vulgaris (T. vulgaris extracts for inhibition of bacteria in planktonic form was confirmed. In dealing with biofilm structures, the inhibitory effect of the extracts was directly correlated to their concentration. Except for the inhibition of biofilm formation, efficacy of each extract was independent from type of solvent. Conclusion: According to the potential of Thymus vulgaris (T. vulgaris extracts to inhibit the test bacteria in planktonic and biofilm form, it can be suggested that Thymus vulgaris(T. vulgaris extracts can be applied as antimicrobial agents against the pathogenic bacteria particularly in biofilm forms.

  5. Gas chromatography-mass spectrometry analysis of different organic crude extracts from the local medicinal plant of Thymus vulgaris L

    Institute of Scientific and Technical Information of China (English)

    Laila Salim Al Hashmi; Mohammad Amzad Hossain; Afaf Mohammed Weli; Qasim Al-Riyami; Jamal Nasser Al-Sabahi

    2013-01-01

    Objective: To isolate and analyze the chemical composition in different crude extracts of from the leaves of locally grown of Thymus vulgaris L (T. vulgaris) by gas chromatography-mass spectrometry (GC-MS). Methods: The shade dried leaves powder was extracted with methanol by using Soxhlet extractor. Methanol crude extracts of T. vulgaris and the derived fractions of hexane, chloroform, ethyl acetate and butanol were obtained. Results: Qualitative analyses of various organic crude extracts of T. vulgaris by using GC-MS showed that there were different types of high and low molecular weight compounds. Most of the isolated and identified compounds by GC-MS in the crude extracts are basically biologically important. Further, the T. vulgaris leaf possessed certain characteristics that can be ascribed to cultivation on a domestic plantation. The crude extracts were prepared from the powder leaves of T. vulgaris for respective compounds can be chosen on the basis of above GC-MS analysis. Conclusions: All the major compounds were identified and characterized by spectroscopic method in different organic crude extracts of T. vulgaris are biologically active molecules. Thus the identification of a good number of compounds in various crude extracts of T. vulgaris might have some ecological role.

  6. Gas chromatography-mass spectrometry analysis of different organic crude extracts from the local medicinal plant of Thymus vulgaris L

    Institute of Scientific and Technical Information of China (English)

    Laila; Salim; Al; Hashmi; Mohammad; Amzad; Hossain; Afaf; Mohammed; Weli; Qasim; Al-Riyami; Jamal; Nasser; Al-Sabahi

    2013-01-01

    Objective:To isolate and analyze the chemical composition in different crude extracts of from the leaves of locally grown of Thymus vulgaris L(T.vulgaris)by gas chromatography-mass spectrometry(GC-MS).Methods:The shade dried leaves powder was extracted with methanol by using Soxhlet extractor.Methanol crude extracts of T.vulgaris and the derived fractions of hexane,chloroform,ethyl acetate and butanol were obtained.Results:Qualitative analyses of various organic crude extracts of T.vulgaris by using GC-MS showed that there were different types of high and low molecular weight compounds.Most of the isolated and identified compounds by GC-MS in the crude extracts are basically biologically important.Further,the T.vulgaris leaf possessed certain characteristics that can be ascribed to cultivation on a domestic plantation.The crude extracts were prepared from the powder leaves of T.vulgaris for respective compounds can be chosen on the basis of above GC-MS analysis.Conclusions:All the major compounds were identified and characterized by spectroscopic method in different organic crude extracts of T.vulgaris are biologically active molecules.Thus the identification of a good number of compounds in various crude extracts of T.vulgaris might have some ecological role.

  7. Gas chromatography–mass spectrometry analysis of different organic crude extracts from the local medicinal plant of Thymus vulgaris L

    Directory of Open Access Journals (Sweden)

    Laila Salim Al Hashmi

    2013-01-01

    Conclusions: All the major compounds were identified and characterized by spectroscopic method in different organic crude extracts of T. vulgaris are biologically active molecules. Thus the identification of a good number of compounds in various crude extracts of T. vulgaris might have some ecological role.

  8. Atividade antimicrobiana de Struthanthus vulgaris (erva-de-passarinho Antimicrobial activity of Struthantus vulgaris (erva-de-passarinho

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    O.M.C. Vieira

    2005-06-01

    Full Text Available As plantas do gênero Struthanthus são conhecidas como ervas-de-passarinho e parasitam pomares no Brasil, principalmente os de laranjeiras e goiabeiras. Na medicina popular são usadas nas afecções das vias respiratórias. O extrato hidroetanólico a 70% de folhas frescas de Struthanthus vulgaris apresentou atividade antimicrobiana contra amostras bacterianas Gram positiva e Gram negativa. Este extrato não apresentou, nas condições testadas, atividade contra fungos. As amostras bacterianas mais sensíveis ao extrato foram Bacillus cereus (ATCC 11778, Micrococcus luteus (ATCC 9341, Staphylococcus aureus (ATCC 6538, S. epidermidis (ATCC 12228 e P. aeruginosa (ATCC 27853, usando o método de difusão em agar. As frações obtidas, pela partição líquido-líquido do extrato hidroetanólico a 70%, com solventes de polaridades crescentes (clorofórmio, acetato de etila, n-butanol e água, apresentaram diferentes atividades inibitórias. A fração que apresentou a maior atividade contra bactéria Gram positiva (B. cereus e Gram negativa (P. aeruginosa foi aquela obtida com n-butanol. Nessa fração foram detectados flavonóides, taninos condensados (proantocianidinas e saponinas.Struthantus vulgaris (mistletoe is one of the most common hemiparasite species in Brazil. It occurs as a parasite of orchards, mainly in orange and guava trees. Some authors mention Struthantus use in traditional medicine for respiratory diseases treatment. Fresh leaves concentrated hydroalcoholic extract showed antimicrobial activity against Gram positive and Gram negative bacterial samples. In tested conditions, these extracts did not show activity against fungi. The more susceptible bacterial samples to fresh leaves hydroalcoholic extract were Bacillus cereus (ATCC 11778, Micrococcus luteus (ATCC 9341, Staphylococcus aureus (ATCC 6538, S. epidermidis (ATCC 12228 and Pseudomonas aeruginosa (ATCC 27853. The method used for assessment of antimicrobial activity was agar

  9. Use of diluted urine for cultivation of Chlorella vulgaris.

    Science.gov (United States)

    Jaatinen, Sanna; Lakaniemi, Aino-Maija; Rintala, Jukka

    2016-01-01

    Our aim was to study the biomass growth of microalga Chlorella vulgaris using diluted human urine as a sole nutrient source. Batch cultivations (21 days) were conducted in five different urine dilutions (1:25-1:300), in 1:100-diluted urine as such and with added trace elements, and as a reference, in artificial growth medium. The highest biomass density was obtained in 1:100-diluted urine with and without additional trace elements (0.73 and 0.60 g L(-1), respectively). Similar biomass growth trends and densities were obtained with 1:25- and 1:300-diluted urine (0.52 vs. 0.48 gVSS L(-1)) indicating that urine at dilution 1:25 can be used to cultivate microalgal based biomass. Interestingly, even 1:300-diluted urine contained sufficiently nutrients and trace elements to support biomass growth. Biomass production was similar despite pH-variation from < 5 to 9 in different incubations indicating robustness of the biomass growth. Ammonium formation did not inhibit overall biomass growth. At the beginning of cultivation, the majority of the biomass consisted of living algal cells, while towards the end, their share decreased and the estimated share of bacteria and cell debris increased.

  10. Indocyanine green-laser thermolysis of acne vulgaris

    Science.gov (United States)

    Genina, Elina A.; Bashkatov, Alexey N.; Simonenko, Georgy V.; Tuchin, Valery V.; Yaroslavsky, Ilya V.; Altshuler, Gregory B.

    2005-08-01

    The near-infrared (NIR) laser radiation due to its high penetration depth is widely used in phototherapy and photothermolysis. In application to skin appendages a high selectivity of laser treatment is needed to prevent light action on surrounding tissues. Indocyanine Green (ICG) dye may provide a high selectivity of treatment due to effective ICG uploading by a target and its narrow band of considerable absorption just at the wavelength of the NIR diode laser. The goal of this study is to demonstrate the efficacy of the NIR diode laser photothermolysis in combination with topical application of ICG suggested for treatment of acne vulgaris. Two volunteers with back-located acne were enrolled. Skin sites of subjects were stained by ICG and irradiated by NIR laser-diode light (803 or 809 nm). The individual acne lesions were photothermally treated at 18 W/cm2 (803 nm, 0.5 sec) without skin surface cooling or at 200 W/cm2 (809 nm, 0.5 sec) with cooling. The results of the observations during a month after the treatment have shown that ICG stained acne inflammatory elements were destructed for light exposures of 0.5 sec.

  11. Structure and mechanical properties of Octopus vulgaris suckers.

    Science.gov (United States)

    Tramacere, Francesca; Kovalev, Alexander; Kleinteich, Thomas; Gorb, Stanislav N; Mazzolai, Barbara

    2014-02-06

    In this study, we investigate the morphology and mechanical features of Octopus vulgaris suckers, which may serve as a model for the creation of a new generation of attachment devices. Octopus suckers attach to a wide range of substrates in wet conditions, including rough surfaces. This amazing feature is made possible by the sucker's tissues, which are pliable to the substrate profile. Previous studies have described a peculiar internal structure that plays a fundamental role in the attachment and detachment processes of the sucker. In this work, we present a mechanical characterization of the tissues involved in the attachment process, which was performed using microindentation tests. We evaluated the elasticity modulus and viscoelastic parameters of the natural tissues (E ∼ 10 kPa) and measured the mechanical properties of some artificial materials that have previously been used in soft robotics. Such a comparison of biological prototypes and artificial material that mimics octopus-sucker tissue is crucial for the design of innovative artificial suction cups for use in wet environments. We conclude that the properties of the common elastomers that are generally used in soft robotics are quite dissimilar to the properties of biological suckers.

  12. Activity of Thymus vulgaris essential oil against Anisakis larvae.

    Science.gov (United States)

    Giarratana, F; Muscolino, D; Beninati, C; Giuffrida, A; Panebianco, A

    2014-07-01

    Anisakiasis is an important food-borne disease especially in countries with high fish consumption. The increase of cases of human disease and the virtual absence of effective treatments have prompted the research on new active compounds against Anisakis larvae. As well known, the disease is related to the consumption of raw or almost raw seafood products, but also marinated and/or salted fishery products, if the processing is insufficient to destroy nematode larvae can represent a risks for the consumers. In the light of the biocidal efficacy against different pathogens demonstrated for various essential oils, the aim of this work is to evaluate the effect of Thymus vulgaris essential oil (TEO) against anisakidae larvae. The TEO at 10% and 5% concentration in oil sunflower seeds, caused in vitro the death of all larvae within 14 h, with cuticle and intestinal wall damages. The results obtained showing a significant activity against Anisakis larvae, suggest further investigation on TEO as a larvicidal agent and on its potential use in the industrial marinating process. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. Antinociceptive effects of hydroalcoholic extract of Thymus vulgaris.

    Science.gov (United States)

    Taherian, Abbas Ali; Babaei, Mahdi; Vafaei, Abbas Ali; Jarrahi, Morteza; Jadidi, Majid; Sadeghi, Hassan

    2009-01-01

    Previous investigation has shown that Thymus Vulgaris (TV) modulates pain. The aim of this work was to examine the role of TV on acute and chronic pain and compares its effect with dexamethasone (DEX) and stress (ST) by using hot plate, tail flick and formalin tests in mice. In this study male albino mice (25-30 g.) in 21 groups (n=147) were used. TV (100, 500 and 1000 mg/kg), DEX (0.5, 1 and 2 mg/kg) and vehicle (VEH) were injected 30 minutes before pain assessment tests. Stress was applied by 1 min swimming in cold water (18-22 degrees ). Acute and chronic pain was assessed by hot plate, tail flick and formalin tests. For assessment of the role of opioid receptors in antinoceception of TV extract, Naloxon (NAL, 2mg/kg, ip) as opioid receptor antagonist was injected before the injection of the more effective dose (500 mg/kg) of TV extract. Results indicated that TV, DEX and ST have analgesic effects in all tests (P<0.01 in comparison with control group). Above findings showed that TV extract, DEX and ST have modulatory effects on acute and chronic pain. Further research is required to determine the mechanisms by which TV extract has an inhibitory effect on pain sensation.

  14. Evaluation of solar dried thyme (Thymus vulgaris Linne) herbs

    Energy Technology Data Exchange (ETDEWEB)

    Balladin, Derrick A.; Headley, Oliver [University of the West Indies, Center for Resource Management and Environmental Studies, St Michael (Barbados)

    1999-07-01

    Thyme (Thymus vulgaris Linne) herbs can be dried at about 50degC reached an equilibrium moisture content after 12 h and 9.5 h using the wire basket solar dryer and oven drying method respectively. The initial moisture content (wet wt. basis), (final moisture content, dry wt. basis (dwb)) determined by the Dean-Stark toluene method, oven and microwave were 75.15% (10.0%), 75.12% (11.85%) and 72.31% (12.50%) respectively. Paired t-test ({alpha} = 0.05, 10 degrees of freedom) showed no significant difference between the Dean-Stark toluene and the oven methods, but a significant difference between these two methods and the microwaves method. The % essential oils extracted after drying by the oven and the wire basket solar methods were 0.5 and 0.6% (per 100 g dwb) respectively. The % oleoresin and ash content were 27% for both drying methods and 1.60, 2.03 and 2.25% for the fresh, oven dried and the wire basket solar dried herb respectively. (Author)

  15. Antioxidative effect of thyme (Thymus vulgaris in sunflower oil

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    Zofia Zaborowska

    2012-09-01

    Full Text Available   Background. Lipid oxidation is a main problem during food processing, storage and consumption leading to losses of quality, stability, safety and nutritive value. Antioxidants have been used to prevent oxidation changes and off – flavor development in food products. Aim of the research was to evaluate antioxidative effect of thyme ethanol extract on sunflower oil during its storage in different temperature conditions. Oil samples were stored in darkness at 4°C, 18°C, 38°C. Material and methods. Samples of thyme (thymus vulgaris were purchased at a local pharmacy in Poznań, Poland and sunflower oil was acquired from a local supermarket. Thyme extract was characterized by total polyphenol content. Antioxidant activity was estimated with use of DPPHand ABTSradicals scavenging methods. Ethanol extract of thyme at 1% level was added to sunflower oil. Peroxide value (PV, anisidine value (AV, totox value (TxV and fatty acids (FA content were taken as parameters for evaluation of effectiveness of thyme extract in stabilization of sunflower oil. Results. High polyphenol content, DPPHand ABTSradicals scavenging activity of ethanol thyme extract were evaluated. Results from different parameters were in agreement with other researchers, suggesting the antioxidant effect of thyme on antioxidant stability. Results show that thyme extract prolonged stability of sunflower oil and it may be a potent antioxidant for its stabilization. Conclusions. Ethanol thyme extract may be used as a natural antioxidant to prolong stability of oils.  

  16. Signaling dependent and independent mechanisms in pemphigus vulgaris blister formation.

    Science.gov (United States)

    Saito, Masataka; Stahley, Sara N; Caughman, Christopher Y; Mao, Xuming; Tucker, Dana K; Payne, Aimee S; Amagai, Masayuki; Kowalczyk, Andrew P

    2012-01-01

    Pemphigus vulgaris (PV) is an autoimmune epidermal blistering disease caused by autoantibodies directed against the desmosomal cadherin desmoglein-3 (Dsg3). Significant advances in our understanding of pemphigus pathomechanisms have been derived from the generation of pathogenic monoclonal Dsg3 antibodies. However, conflicting models for pemphigus pathogenicity have arisen from studies using either polyclonal PV patient IgG or monoclonal Dsg3 antibodies. In the present study, the pathogenic mechanisms of polyclonal PV IgG and monoclonal Dsg3 antibodies were directly compared. Polyclonal PV IgG cause extensive clustering and endocytosis of keratinocyte cell surface Dsg3, whereas pathogenic mouse monoclonal antibodies compromise cell-cell adhesion strength without causing these alterations in Dsg3 trafficking. Furthermore, tyrosine kinase or p38 MAPK inhibition prevents loss of keratinocyte adhesion in response to polyclonal PV IgG. In contrast, disruption of adhesion by pathogenic monoclonal antibodies is not prevented by these inhibitors either in vitro or in human skin explants. Our results reveal that the pathogenic activity of polyclonal PV IgG can be attributed to p38 MAPK-dependent clustering and endocytosis of Dsg3, whereas pathogenic monoclonal Dsg3 antibodies can function independently of this pathway. These findings have important implications for understanding pemphigus pathophysiology, and for the design of pemphigus model systems and therapeutic interventions.

  17. An antifungal defensin from Phaseolus vulgaris cv. 'Cloud Bean'.

    Science.gov (United States)

    Wu, Xiangli; Sun, Jian; Zhang, Guoqing; Wang, Hexiang; Ng, Tzi Bun

    2011-01-15

    An antifungal peptide with a defensin-like sequence and exhibiting a molecular mass of 7.3kDa was purified from dried seeds of Phaseolus vulgaris 'Cloud Bean'. The isolation procedure entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography an Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Although the antifungal peptide was unadsorbed on DEAE-cellulose, it was adsorbed on both Affi-gel blue gel and SP-Sepharose. The antifungal peptide exerted antifungal activity against Mycosphaerella arachidicola with an IC(50) value of 1.8 μM. It was also active against Fusarium oxysporum with an IC(50) value of 2.2 μM. It had no inhibitory effect on HIV-1 reverse transcriptase when tested up to 100 μM. Proliferation of L1210 mouse leukemia cells and MBL2 lymphoma cells was inhibited by the antifungal peptide with an IC(50) of 10 μM and 40 μM, respectively.

  18. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean

    Institute of Scientific and Technical Information of China (English)

    Yau Sang Chan; Jack Ho Wong; Evandro Fei Fang; Wen Liang Pan; Tzi Bun Ng

    2012-01-01

    A 5.4-kDa antifungal peptide,with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC5o=3 μM),Setospaeria turcica and Bipolaris maydis,was isolated from the seeds of Phaseolus vulgaris cv.brown kidney bean.The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75.The antifungal activity of the peptide against M.arachidicola was stable in the pH range 3-12 and in the temperature range 0℃ to 80℃.There was a slight reduction of the antifungal activity at pH 2 and 13,and the activity was indiscernible at pH 0,1,and 14.The activity at 90℃ and 100℃ was slightly diminished.Deposition of Congo red at the hyphal tips of M.arachidicola was induced by the peptide indicating inhibition of hyphal growth.The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells,in contrast to the presence of such activity of other antifungal peptides,indicates that different domains are responsible for the antifungal and antiproliferative activities.

  19. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean.

    Science.gov (United States)

    Chan, Yau Sang; Wong, Jack Ho; Fang, Evandro Fei; Pan, Wen Liang; Ng, Tzi Bun

    2012-04-01

    A 5.4-kDa antifungal peptide, with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC(50)= 3 μM), Setospaeria turcica and Bipolaris maydis, was isolated from the seeds of Phaseolus vulgaris cv. brown kidney bean. The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75. The antifungal activity of the peptide against M. arachidicola was stable in the pH range 3-12 and in the temperature range 0°C to 80°C. There was a slight reduction of the antifungal activity at pH 2 and 13, and the activity was indiscernible at pH 0, 1, and 14. The activity at 90°C and 100°C was slightly diminished. Deposition of Congo red at the hyphal tips of M. arachidicola was induced by the peptide indicating inhibition of hyphal growth. The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells, in contrast to the presence of such activity of other antifungal peptides, indicates that different domains are responsible for the antifungal and antiproliferative activities.

  20. Redox Enzymes of Red Beetroot Vacuoles (Beta vulgaris L.

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    E.V. Pradedova

    2014-12-01

    Full Text Available Years of research have shown that some of the redox elements (enzymes, coenzymes, and co-substrate are isolated from each other kinetic and spatial manner (compartmentalization in the eukaryotic cells. The redox elements forming the "highly" and "widely" specialized redox system are found in all cell structures: mitochondria, plastids, peroxisomes, apoplast, nucleus etc. In recent years the active involvement of the central vacuole in the maintenance of the plant cell redox homeostasis is discussed, actually the information about the vacuolar redox system is very small. The high-priority redox processes and "redox-specialization" of the vacuolar compartment are not known. We have begun a study of red beet-root vacuole redox systems (Beta vulgaris L. and have identified redox enzymes such as: phenol peroxidase (EC 1.11.1.7, superoxide dismutase (EC 1.15.1.1 and glutathione reductase (EC 1.8.1.7. This paper presents some of the characteristics of these enzymes and considers the probable ways of their functioning in vacuolar redox chains.

  1. Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats

    Institute of Scientific and Technical Information of China (English)

    Emey Suhana MOHD AZAMAI; Suhaniza SULAIMAN; Shafina Hanim MOHD HABIB; Mee Lee LOOI; Srijit DAS; Nor Aini ABDUL HAMID; Wan Zurinah WANG NGAH; Yasmin Anum MOHD YUSOF

    2009-01-01

    Chlorella vulgaris (CV) has been reported to have antioxidant and anticancer properties. We evaluated the effect of CV on apoptotic regulator protein expression in liver cancer-induced rats. Male Wistar rats (200-250 g) were divided into eight groups: control group (normal diet), CDE group (choline deficient diet supplemented with ethionine in drinking water to induce hepatocarcinogenesis), CV groups with three different doses of CV (50, 150, and 300 mg/kg body weight), and CDE groups treated with different doses of CV (50, 150, and 300 mg/kg body weight). Rats were sacrificed at various weeks and liver tissues were embedded in paraffin blocks for immunohistochemistry studies. CV, at increasing doses, decreased the expression of anti-apoptotic protein, Bcl-2, but increased the expression of pro-apoptotic protein, caspase 8, in CDE rats, which was correlated with decreased hepatoctyes proliferation and increased apoptosis as determined by bromodeoxy-uridine (BrdU) labeling and terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay, respectively. Our study shows that CV has definite chemopreventive effect by inducing apoptosis via decreasing the expression of Bcl-2 and increasing the expression of caspase 8 in hepatocarcinogenesis-induced rats.

  2. Efficient in vitro micropropagation and regeneration of Artemisia vulgaris L.

    Directory of Open Access Journals (Sweden)

    Sujatha Govindaraj

    2007-01-01

    Full Text Available This paper describes efficient propagation of Artemisia vulgaris using shoot tip explants isolated from 35 daysold in vitro grown seedlings. Optimum proliferation was obtained on Murashige and Skoog’s salts and B5 vitamins supplementedwith 3% sucrose, 4.44 μM BA, and 0.7% agar. Shoot proliferation was maximal (99.8% with 14-23 shoots per explant after6 weeks of culture. Shoots with a minimum length of 1.5 cm were transferred to shoot elongation medium supplemented with0.44 μM BA and 1.44 μM GA3. The successfully elongated shoots with a height of 7.2-12.1 cm were transferred to rootingmedium augmented with 8.56 μM IAA. Rooted plantlets were transferred to plastic cups containing autoclaved garden soil,farmyard soil and sand (2:1:1 for hardening. Plantlets were initially maintained under culture room conditions (5 weeks,followed by normal laboratory conditions (4 weeks and finally transferred to a Botanical Evaluation Garden and maintainedthere.

  3. Inheritance of resistance to ozone in Phaseolus vulgaris L

    Energy Technology Data Exchange (ETDEWEB)

    Butler, L.K.; Tibbitts, T.W.; Bliss, F.A.

    1979-03-01

    Ozone sensitivity was compared in F/sub 1/ and F/sub 2/ populations from crosses between 2 ozone-sensitive bean cultivars, Spurt and Blue Lake Stringless, and 2 ozone-resistant cultivars, Black Turtle Soup and French's Horticultural, under controlled environmental conditions. F/sub 1/ plants were as sensitive as the sensitive parent. About 10% of the F/sub 2/ progeny obtained by selfing F/sub 1/ plants appeared to be as resistant as the resistant parent and 90% of the progeny could be divided equally between a group as sensitive as the sensitive parent and a group intermediate in sensitivity between the parent plants. However, precise separation of F/sub 2/ progeny was not possible because of the variability in injury expression. The average injury on the F/sub 2/ plants was greater than the parental midpoint value and the variance in injury on the F/sub 2/ plants was about 3.5x greater than that for the parents. The heritability of resistance to ozone was estimated to be 0.83. It was concluded that ozone resistance is recessive in P. vulgaris and appears to be regulated by a few major genes. 12 references, 2 figures, 2 tables.

  4. Systemic lupus erythematosus and pemphigus vulgaris: association or coincidence.

    Science.gov (United States)

    Calebotta, A; Cirocco, A; Giansante, E; Reyes, O

    2004-01-01

    Few cases have been published relating systemic lupus erythematosus (SLE) and pemphigus vulgaris (PV). We describe a patient with this association. A 35-year old woman who started to develop persistent pain and morning stiffness of proximal inter and metacarpo-phalangeal joints. During the following year, the patient recalled the onset of blisters on both legs, face, arms and thorax, as well as erosions appearing on oral mucous membranes. We observed generalized multiple erosions on her trunk and legs, flaccid bullae located on her right thigh and multiple erosions on oral mucous membranes. A skin biopsy reported PV Direct immunofluorescence on the perilesional skin specimen, showed beehive intercellular IgG deposits in the epidermis (+++), suggesting PV; granular discontinuous IgM and C3 deposits in the dermal-epidermal union (+++), suggesting SLE. Direct immunofluorescence of the healthy unexposed skin specimen, reported granular discontinuous IgG deposits in the dermal-epidermal union and beehive intercellular IgG deposits in the lower levels of the epidermis (+++); granular continuous IgM deposits in the dermal-epidermal union (+++). The results of rheumatic studies were obtained as follows: ANA :3 +, Anti-DNA, Anti-Sm, Anti-Ro and Anti-La :4 + . The definite diagnosis was PVand SLE. Treatment with 50 mg of prednisone daily with good evolution.

  5. A Rare Case of Cranial Osteomyelitis Caused by Proteus Vulgaris

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    Hakan Uslu

    2011-03-01

    Full Text Available Osteomyelitis of the calvarial bones can cause serious complications such as brain abscess, due to the close proximity to adjacent brain structures. Development of the purulent secretion in surgery and traumatic scalp injuries must be considered as a possibility of osteomyelitis possibility. Generally gram positive, rarely gram negative bacteria and mix agents, can be isolated in infection. Especially chronic pyogenic osteomyelitis agents can be isolated from chronic infections such as tuberculosis. In cranial osteomyelitis diagnosis, radiological diagnosis has a very important place together with the clinical diagnosis. However, infection can usually show late findings radiologically. In treatment, antibiotic treatment is absolutely essential as well as removal of the infected part of the bone. Due to antibiotic treatment lasting between 6-12 weeks, organizing the antibiotic protocols according to the results of culture-antibiograms, which were provided from purulent secretions, has the most important role in the success of surgical treatment. In Proteus sp. infections, for choice of suitable treatment, determination of the type of bacteria is important. For exact diagnosis, histopathological examination of the bone tissue must be carried out. In this report, a case with cranial osteomyelitis caused by Proteus vulgaris which is a gram negative bacteria causing anaerobic infections and classified in the Enterobacteriaceae family is presented. The patient was treated with surgery and appropriate antibiotics. Early recognition of this condition, planning the best treatment strategy and taking precautions to prevent complications, is mandatory for a better outcome.

  6. Family study of monozygotic twins affected by pemphigus vulgaris.

    Science.gov (United States)

    Salathiel, Adriana Martinelli; Brochado, Maria José Franco; Kim, Olivia; Deghaide, Neifi Hassan Saloum; Donadi, Eduardo Antonio; Roselino, Ana Maria

    2016-07-01

    Rare are the family studies that include siblings affected by pemphigus vulgaris (PV) and in whom HLA class II alleles are related. HLA-DR and -DQ genotyping and profiling of antibodies against desmogleins (Dsg) 1 and Dsg3 were performed in ten members of a family including monozygotic twins affected by PV. The twin sisters were heterozygotes; they presented the haplotypes most commonly associated with increased susceptibility to PV (DRB1∗04:02-DQA1∗03:01-DQB1∗03:02 and DRB1∗14:04-DQA1∗01:01-DQB1∗05:03). Their parents and five siblings had only one or none of these two haplotypes in combination with the alleles or haplotypes associated with resistance to PV (DRB1∗07:01-DQA1∗02:01-DQB1∗02:02 and DRB1∗13:01-DQA1∗01:03-DQB1∗06:03). Only the monozygotic twins presented IgG antibodies against both Dsg1 and Dsg3. According to our knowledge based on a review of published literature on the topic, this is the first report of PV affecting monozygotic twins.

  7. Landscape genetics, adaptive diversity and population structure in Phaseolus vulgaris.

    Science.gov (United States)

    Rodriguez, Monica; Rau, Domenico; Bitocchi, Elena; Bellucci, Elisa; Biagetti, Eleonora; Carboni, Andrea; Gepts, Paul; Nanni, Laura; Papa, Roberto; Attene, Giovanna

    2016-03-01

    Here we studied the organization of genetic variation of the common bean (Phaseolus vulgaris) in its centres of domestication. We used 131 single nucleotide polymorphisms to investigate 417 wild common bean accessions and a representative sample of 160 domesticated genotypes, including Mesoamerican and Andean genotypes, for a total of 577 accessions. By analysing the genetic spatial patterns of the wild common bean, we documented the existence of several genetic groups and the occurrence of variable degrees of diversity in Mesoamerica and the Andes. Moreover, using a landscape genetics approach, we demonstrated that both demographic processes and selection for adaptation were responsible for the observed genetic structure. We showed that the study of correlations between markers and ecological variables at a continental scale can help in identifying local adaptation genes. We also located putative areas of common bean domestication in Mesoamerica, in the Oaxaca Valley, and the Andes, in southern Bolivia-northern Argentina. These observations are of paramount importance for the conservation and exploitation of the genetic diversity preserved within this species and other plant genetic resources.

  8. Plasma androgenic activity in women with acne vulgaris and in healthy girls before, during and after puberty.

    Science.gov (United States)

    Odlind, V; Carlström, K; Michaëlsson, G; Vahlqvist, A; Victor, A; Mellbin, T

    1982-03-01

    The possible relationship between plasma androgenic activity and acne vulgaris was investigated. Plasma testosterone and sex hormone binding globulin (SHBG) levels were determined in healthy girls during different stages of puberty, in healthy adult women and in women with acne vulgaris. Testosterone increase during puberty, whereas SHBG decreased during the early stages before it increased and stabilized plasma concentrations of testosterone and SHBG. Women with severe acne vulgaris had testosterone levels in the same range but the SHBG levels were significantly lower than those of healthy women and women with mild acne. These results show a high androgenic activity in the intermediate stages of puberty, when acne vulgaris is a common complaint and an increased androgenic activity in adult women with severe acne vulgaris.

  9. Lipid accumulation from pinewood pyrolysates by Rhodosporidium diobovatum and Chlorella vulgaris for biodiesel production.

    Science.gov (United States)

    Luque, Luis; Orr, Valerie C A; Chen, Sean; Westerhof, Roel; Oudenhoven, Stijn; Rossum, Guus van; Kersten, Sascha; Berruti, Franco; Rehmann, Lars

    2016-08-01

    This study evaluated the suitability of pinewood pyrolysates as a carbon source for lipid production and cultivation of the oleaginous yeast Rhodosporidium diobovatum and the microalgae Chlorella vulgaris. Thermal decomposition of pinewood and fractional condensation were used to obtain an oil rich in levoglucosan which was upgraded to glucose by acid hydrolysis. Blending of pyrolytic sugars with pure glucose in both nitrogen rich and nitrogen limited conditions was studied for R. diobovatum, and under nitrogen limited conditions for C. vulgaris. Glucose consumption rate decreased with increasing proportions of pyrolytic sugars increasing cultivation time. While R. diobovatum was capable of growth in 100% (v/v) pyrolytic sugars, C. vulgaris growth declined rapidly in blends greater than 20% (v/v) until no growth was detected in blends >40%. Finally, the effects of pyrolysis sugars on lipid composition was evaluated and biodiesel fuel properties were estimated based on the lipid profiles.

  10. Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E.

    Science.gov (United States)

    Ho, Shih-Hsin; Huang, Shu-Wen; Chen, Chun-Yen; Hasunuma, Tomohisa; Kondo, Akihiko; Chang, Jo-Shu

    2013-05-01

    In this study, three indigenous microalgae isolates were examined for their ability to produce carbohydrates. Among them, Chlorella vulgaris FSP-E displayed relatively high cell growth rate and carbohydrate content. The carbohydrate productivity of C. vulgaris FSP-E was further improved by using engineering strategies. The results show that using an appropriate light intensity and inoculum size could effectively promote cell growth and carbohydrate productivity. Nitrogen starvation triggered the accumulation of carbohydrates in the microalga, achieving a carbohydrate content of 51.3% after 4-day starvation. Under the optimal conditions, the highest biomass and carbohydrate productivity were 1.437 and 0.631 g L(-1) d(-1), respectively. This performance is better than that reported in most related studies. Since glucose accounted for nearly 93% of the carbohydrates accumulated in C. vulgaris FSP-E, the microalga is an excellent feedstock for bioethanol fermentation.

  11. Caleosin from Chlorella vulgaris TISTR 8580 is salt-induced and heme-containing protein.

    Science.gov (United States)

    Charuchinda, Pairpilin; Waditee-Sirisattha, Rungaroon; Kageyama, Hakuto; Yamada, Daisuke; Sirisattha, Sophon; Tanaka, Yoshito; Mahakhant, Aparat; Takabe, Teruhiro

    2015-01-01

    Physiological and functional properties of lipid droplet-associated proteins in algae remain scarce. We report here the caleosin gene from Chlorella vulgaris encodes a protein of 279 amino acid residues. Amino acid sequence alignment showed high similarity to the putative caleosins from fungi, but less to plant caleosins. When the C. vulgaris TISTR 8580 cells were treated with salt stress (0.3 M NaCl), the level of triacylglycerol increased significantly. The mRNA contents for caleosin in Chlorella cells significantly increased under salt stress condition. Caleosin gene was expressed in E. coli. Crude extract of E. coli cells exhibited the cumene hydroperoxide-dependent oxidation of aniline. Absorption spectroscopy showed a peak around 415 nm which was decreased upon addition of cumene hydroperoxide. Native polyacrylamide gel electrophoresis suggests caleosin existed as the oligomer. These data indicate that a fresh water C. vulgaris TISTR 8580 contains a salt-induced heme-protein caleosin.

  12. In Vitro Antimicrobial Activity of Thymus vulgaris Essential Oil Against Major Oral Pathogens.

    Science.gov (United States)

    Fani, Mohammadmehdi; Kohanteb, Jamshid

    2017-01-01

    The objective of present investigation was to determine antimicrobial activity of Thymus vulgaris oil on some oral pathogens. Thymus vulgaris oil was prepared by hydrodistillation and tested against 30 clinical isolates of each of Streptococcus pyogenes, Streptococcus mutans, Candida albicans, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans, prepared from related oral infections using agar disk diffusion and broth microdilution methods. Thymus vulgaris oil at concentrations of 16 to 256 μg/mL exhibited strong inhibitory activity on all clinical isolates producing inhibition zones of 7.5 to 42 mm as measured by agar disk diffusion method. Streptococcus pyogenes and Streptococcus mutans were the most sensitive isolates with minimum inhibitory concentrations of 1.9 and 3.6 μg/mL, respectively. The minimum inhibitory concentration values for C albicans, A actinomycetemcomitans, and P gingivalis were 16.3, 32, and 32 μg/mL, respectively.

  13. Antispasmodic effect of hydroalcoholic extract of Thymus vulgaris on the guinea-pig ileum.

    Science.gov (United States)

    Babaei, Mehdi; Abarghoei, Mitra Emmami; Ansari, Reza; Vafaei, Abbas Ali; Taherian, Abbas Ali; Akhavan, Maziar Mohammad; Toussy, Gafar; Mousavi, Shahrokh

    2008-01-01

    The effects of Thymus vulgaris hydroalcoholic extract on the contractile responses of the isolated guinea-pig ileum were investigated. Contraction changes in the terminal ileum of guinea pigs were monitored using a force displacement transducer amplifier connected to a physiograph. Thymus vulgaris extract inhibited the contractile responses in a dose-dependent manner and also decreased the amplitude of peristaltic waves. It is concluded that T. vulgaris has an antispasmodic action on guinea pig ileum by decreasing the amplitudes of the muscle contractions during peristalsis. The EC50 was calculated as 1.7 mg mL(-1). In guinea-pig ileum the extract led to an antispasmodic effect, possibly by affecting the anticholinergic and serotoninergic pathways.

  14. Topical, Biological and Clinical Challenges in the Management of Patients with Acne Vulgaris

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    Anwar Al-Hammadi

    2016-05-01

    Full Text Available Acne vulgaris is one of the most common chronic inflammatory skin disorders among adolescents and young adults. It is associated with substantial morbidity and, rarely, with mortality. The exact worldwide incidence and prevalence are currently unknown. Current challenges involve improving understanding of the underlying pathophysiology of acne vulgaris and developing a practical treatment consensus. Expert panel discussions were held in 2013 and 2014 among a group of scientists and clinicians from the Omani and United Arab Emirate Dermatology Societies to ascertain the current optimal management of acne vulgaris, identify clinically relevant end-points and construct suitable methodology for future clinical trial designs. This article reviews the discussions of these sessions and recent literature on this topic.

  15. A comparative study of phytohaemagglutinin and extract of Phaseolus vulgaris seeds by characterization and cytogenetics

    Science.gov (United States)

    Badari Nath, A. R. S.; Sivaramakrishna, A.; Marimuthu, K. M.; Saraswathy, Radha

    2015-01-01

    Phytohaemagglutinin (PHA) is a lectin obtained from Phaseolus vulgaris (red kidney beans), that acts as a mitogen in human leucocyte culture and is commercially available from Gibco®. This PHA (Gibco®) was found to be very expensive, hence other inexpensive sources that can be used in all kinds of cytogenetics labs (rich and poor), were attempted. One such successful attempt was PHA extract from seeds of P.vulgaris. This paper details the methodology of extraction and application of PHA from seeds of P.vulgaris. Attempts has been made to identify the chemical and physical properties of the products in the extract, analyzed by various spectroscopic and analytical techniques. The analysis clearly indicates that the product from Phaseolus seeds extract was found to be similar to the commercially available PHA (Gibco®) in the cytogenetic study of human leucocyte cultures. The present study enforces the possible utility of the plant extract directly for human leucocyte cultures.

  16. Mangifera indica and Phaseolus vulgaris in the bioindication of air pollution in Bahia, Brazil.

    Science.gov (United States)

    Lima, J S; Fernandes, E B; Fawcett, W N

    2000-07-01

    In this article are reported the results of a study on the concentration of ascorbic acid (AA) in Mangifera indica as passive monitor and in Phaseolus vulgaris as active monitor with the intention to study the effects of industrial emissions from the Petrochemical Complex of Camaçari (PCC), Bahia, Brazil, on the vegetation. Leaves from M. indica were collected in two sites in the region under direct influence of industrial emissions and in one presumed nonpolluted reference (background) site. Pots with P. vulgaris were exposed in the same sites. The AA increase in the leaves of M. indica from PCC sites indicates a stress situation. The small AA increase in the P. vulgaris exposed in the more polluted site indicates that the active monitor is a sensitive one. The decrease of its leaf area indicates the inability of this species to activate physiological protection mechanisms like an increase in AA production.

  17. Clinical Observation on Effect of Triptolide Tablet in Treating Patients with Psoriasis Vulgaris

    Institute of Scientific and Technical Information of China (English)

    WU Shao-xi; GUO Ning-ru

    2005-01-01

    Objective: To investigate the effects of triptolide tablet in the treatment of patients with psoriasis vulgaris. Methods: By an open clinical study of 103 patients with psoriasis vulgaris. Psoriasis area severity index (PASI) was measured and recorded before and after treatment for efficacy evaluation. Results:Of the 103 patients, markedly effective was got in 41 (39.7%), improved in 37 (35.8%) and ineffective in 25 (24.5 % ), the total effective rate being 75.7%, and the adverse reaction was shown only in few patients with decreased WBC during the treatment period. Conclusion: Triptolide tablet is effective for the treatment of psoriasis vulgaris during the one-year follow-up.

  18. Antifungal activity of extracts of Rosmarinus officinalis and Thymus vulgaris against Aspergillus flavus and A. ochraceus.

    Science.gov (United States)

    Centeno, S; Calvo, M A; Adelantado, C; Figueroa, S

    2010-05-01

    The antifungal activity of ethanolic extracts of Rosmarinus officinalis and Thymus vulgaris were tested against strains of Aspergillus flavus and A. ochraceus, since these two species are common contaminants of cereals and grains and are able to produce and accumulate mycotoxins. The methodology used is based on measuring the inhibition halos produced by discs impregnated with the extracts and establishing their Minimum Inhibitory Concentration (MIC) as well as the Minimum Fungicide Concentration (MFC). The results obtained suggest that the assayed extracts affect the proper development of A. flavus and A. ochraceus; leading to a lower MIC (1200 ppm) and MFC (2400 ppm) for T. vulgaris extract against A. ochraceus than against A. flavus. The results show, that the extracts of Rosmarinus officinalis and Thymus vulgaris used at low concentrations could have significant potential for the biological control of fungi in foodstuffs.

  19. Removal Cu(II ions from water using sulphuric acid treated Lagenaria vulgaris Shell (Cucurbitaceae

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    Ljupković, R.B.

    2011-09-01

    Full Text Available Removal of Cu(II ions from water solutions by sulphuric acid treated Lagenaria vulgaris shell (ccLVB was studied. Batch experiments were done by shaking a fixed mass of biosorbent (1.0 g with 250 cm3 of 50.0 mg dm–3 Cu(II solutions, at pH ranged from 2 up to 6. Metal concentration in the filtrates as well as in the initial solution was determined by flame atomic absorption spectrometry. Results show that efficiency of Cu(II ions uptake by sulphuric acid treated Lagenaria vulgaris shell is significantly greater than raw Lagenaria vulgaris biosorbent. In addition, there is no significant effect of initial pH of solution on Cu(II ions uptake by ccLVB and obtained biosorbent can be applied in a wide range of pH.

  20. Topical, Biological and Clinical Challenges in the Management of Patients with Acne Vulgaris

    Science.gov (United States)

    Al-Hammadi, Anwar; Al-Ismaily, Abla; Al-Ali, Sameer; Ramadurai, Rajesh; Jain, Rishi; McKinley-Grant, Lynn; Mughal, Tariq I.

    2016-01-01

    Acne vulgaris is one of the most common chronic inflammatory skin disorders among adolescents and young adults. It is associated with substantial morbidity and, rarely, with mortality. The exact worldwide incidence and prevalence are currently unknown. Current challenges involve improving understanding of the underlying pathophysiology of acne vulgaris and developing a practical treatment consensus. Expert panel discussions were held in 2013 and 2014 among a group of scientists and clinicians from the Omani and United Arab Emirate Dermatology Societies to ascertain the current optimal management of acne vulgaris, identify clinically relevant end-points and construct suitable methodology for future clinical trial designs. This article reviews the discussions of these sessions and recent literature on this topic. PMID:27226905

  1. Topical, Biological and Clinical Challenges in the Management of Patients with Acne Vulgaris.

    Science.gov (United States)

    Al-Hammadi, Anwar; Al-Ismaily, Abla; Al-Ali, Sameer; Ramadurai, Rajesh; Jain, Rishi; McKinley-Grant, Lynn; Mughal, Tariq I

    2016-05-01

    Acne vulgaris is one of the most common chronic inflammatory skin disorders among adolescents and young adults. It is associated with substantial morbidity and, rarely, with mortality. The exact worldwide incidence and prevalence are currently unknown. Current challenges involve improving understanding of the underlying pathophysiology of acne vulgaris and developing a practical treatment consensus. Expert panel discussions were held in 2013 and 2014 among a group of scientists and clinicians from the Omani and United Arab Emirate Dermatology Societies to ascertain the current optimal management of acne vulgaris, identify clinically relevant end-points and construct suitable methodology for future clinical trial designs. This article reviews the discussions of these sessions and recent literature on this topic.

  2. Severe multi-resistant pemphigus vulgaris: prolonged remission with a single cycle of rituximab.

    Science.gov (United States)

    Corral, Isabela Soubhia; Freitas, Thais Helena Proença de; Aquino, Renata Telles Rudge de; Koller, Daniella Abbruzzini S; Magliari, Maria Elisa Ruffolo; Muller, Helena

    2013-01-01

    Pemphigus vulgaris is an autoimmune bullous disease whose therapy is based on systemic corticosteroids, with or without immunosuppressants. Rituximab is a chimeric monoclonal antibody of the IgG class, directed at a specific CD20 B cell surface antigen, used in pemphigus vulgaris empirically since 2002, with success in 90% of the cases and long periods of remission. Male patient, 33 years old, diagnosed with pemphigus vulgaris, confirmed by histopathology and direct immunofluorescence. He was treated for seven months with numerous treatments, including immunosuppressive drugs, with an unsatisfactory response, until he had complete remission with the use of rituximab. During a 34-month follow-up period, the patient presented a slight clinical relapse, which was successfully controlled with prednisone in a daily dose of 120 mg, soon reduced to 20mg.

  3. Influence of green algae Chlorella vulgaris on initial growth of different agricultural crops

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    Hajnal-Jafari Timea I.

    2016-01-01

    Full Text Available The aim of this research was to evaluate the effect of green algae Chlorella vulgaris on the initial growth of wheat, maize, bean and lettuce and the microbiological activity of rhizospheric soil. The experiment was conducted in controlled conditions. The inocula were applied as foliar fertilizer by spraying. Plant material was taken 30 days after plant emergance. Chlorella vulgaris affected positively the length (28.5% increase and fresh mass (17.9% increase of maize root, stem length of wheat (24.2% increase and stem mass of lettuce (56.34% increase. Application of Chlorella vulgaris led to the increase of the total number of bacteria and the number of aminoheterotrophs in the maize rhizosphere, total bacterial number in the wheat rhizosphere, and the number of fungi in the rhizosphere of bean. The number of other investigated groups of microorganisms did not change significantly. The activity of dehydrogenase enzyme was not affected by inoculation with green algae.

  4. [Effect of inorganic carbon source on lipid production with autotrophic Chlorella vulgaris].

    Science.gov (United States)

    Zheng, Hongli; Gao, Zhen; Zhang, Qi; Huang, He; Ji, Xiaojun; Sun, Honglei; Dou, Chang

    2011-03-01

    We studied the effects of three inorganic carbon sources, Na2CO3, NaHCO3 and CO2, and their initial concentrations on lipid production of Chlorella vulgaris. Chlorella vulgaris could utilize Na2CO3, NaHCO3 and CO2 to produce lipids. After 10-day cultivation with each of the three inorganic carbon sources, lipid yield of Chlorella vulgaris reached its peak with the concentration increase of the inorganic carbon source, but dropped again by further increase of the concentration. The pH value of the culture medium for Chlorella vulgaris increased after the cultivation on inorganic carbon source. The optimal concentration of both Na2CO3 and NaHCO3 was 40 mmol/L, and their corresponding biomass dry weight was 0.52 g/L and 0.67 g/L with their corresponding lipid yield 0.19 g/L and 0.22 g/L. When the concentration of CO2 was 6%, Chlorella vulgaris grew the fastest and its biomass dry weight was 2.42 g/L with the highest lipid yield of 0.72 g/L. When the concentration of CO2 was too low, the supply of inorganic carbon was insufficient and lipid yield was low. A too high concentration of CO2 caused a low pH and lipid accumulation was inhibited. Na2CO3 and NaHCO3 were more favorable for Chlorella vulgaris to accumulate unsaturated fatty acids than that of CO2.

  5. Toxicological Response of the Green Alga Chlorella vulgaris, to Some Heavy Metals

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    E. Afkar

    2010-01-01

    Full Text Available Problems statement: The disturbance of aquatic ecosystems provoked by heavy metals pollution from industrial and domestic sources, has as consequence the loss of biological diversity, as well as increased bioaccumulation and magnification of toxicants in the food chain. The aim of this study was to evaluate the effect of some heavy metals on some physiological activities of Chlorella vulgaris beyerinck with special references to metal bioaccumulation. Approach: Chlorella vulgaris Beyerinck was isolated from Al-Asfar Lake, Al-Hassa, Saudi Arabia. A standard initial inoculum of the isolated algae was inoculated to culture flasks. The culture flasks were supplied with various concentrations of Cobalt, Copper and Zinc ranging from 10-6-10-9 M. At the end of the incubation period cultures were filtered and washed several times by distilled water for measurements the various experimental parameters. Results: The data show that the lower doses of the three tested metals had stimulatory effect in biomass yield of Chlorella vulgaris, whereas the higher doses were inhibitory depending on the type of the metal. The inhibitory effect of copper to the growth parameters of Chlorella vulgaris was more pronounced than other two tested metals. The total protein content, total carbohydrate and the total free amino acids of the tested green alga Chlorella vulgaris gradually decreased in a manner dependent on the metal concentration in the medium. On the other hand, bioaccumulation of cobalt, copper and zinc by Chlorella vulgaris cells were parallel to increasing the concentrations in the culture medium. Conclusion: The inhibitory and stimulatory effects of either of the used heavy metals depend on concentration. Different organisms, however, have different sensitivities to the same metal and the same organisms may be more or less damaged by different metals. The uptake of an element from the surrounding medium is seldom exactly proportional to the amount present

  6. Inhibition of the catalase activity from Phaseolus vulgaris and Medicago sativa by sodium chloride.

    Science.gov (United States)

    Tejera García, Noel A; Iribarne, Carmen; Palma, Francisco; Lluch, Carmen

    2007-08-01

    Changes in catalase activity during the development of the Rhizobium-legume symbiosis as well as its response in salinized plants of Phaseolus vulgaris and Medicago sativa, was studied. Besides, it was examined the behavior of the enzyme, isolated from leaves and root nodules, during in vitro incubation with NaCl doses. Nodule catalase activities of both legumes were assayed with several enzyme inhibitors and also purified. Leaf catalase activity of Phaseolus vulgaris and Medicago sativa decreased and increased respectively throughout the ontogeny, but root nodule catalase kept a high and stable value. This last result suggests that both legumes require the maintenance of high nodule catalase in nitrogen-fixing nodules. Under salt stress conditions leaf and nodule catalase activity decreased in both, grain and pasture legumes. Because catalase from leaf of Medicago sativa and nodules of Phaseolus vulgaris were relatively sensitive to NaCl during in vitro experiments, the detoxifying role of this enzyme for H(2)O(2) should be limited in such conditions. Both catalases, from determinate and indeterminate nodules, were affected neither by oxygen nor superoxide radicals but showed a strong (Phaseolus vulgaris) or partial (Medicago sativa) inhibition with dithiothreitol, dithionite and beta-mercaptoethanol. Besides, cyanide was the most potent inhibitor of nodule catalases. Finally, catalases partially purified by immobilized metal ion affinity chromatography migrated at 42 (Phaseolus vulgaris) and 46kDa (Medicago sativa) on SDS-PAGE, whereas native forms on sephacryl S-300 columns exhibited a molecular mass of 59 and 48kDa (Phaseolus vulgaris) and 88 and 53kDa (Medicago sativa).

  7. Evaluation of leptin, adiponectin, and ghrelin levels in patients with acne vulgaris.

    Science.gov (United States)

    Ozuguz, P; Kacar, S D; Asik, G; Ozuguz, U; Karatas, S

    2016-02-09

    The research evaluating adipokines are very few in patients with acne vulgaris. The hypothesis that hyperinsulinemic and high glycemic index diet plays a role in the pathogenesis of acne is still controversial. In this study, we aimed to evaluate adipokines such as leptin (L), adiponectin (A), ghrelin and A levels, and A/L rates that indicate insulin resistance in nonobese patients with severe acne vulgaris. Thirty patients who are nonobese with moderate acne vulgaris, aged 18 to 25 years, and 15 age-sex compatible controls were included in our study. The acne lesions were assessed using the Global Acne Grading Scale (GAGS). All participants were evaluated for the parameters that may affect the metabolism of serum L, A, and ghrelin levels in blood, and their body mass index were calculated. The significance level was determined as p ≤ 0.05. Of the 30 patients, 17 were women and 13 were men. The mean age was 20.60 years and the mean duration of the disease were 2.8 years. All of patients had moderate acne vulgaris (GAGS 19-30). Of the 15 controls, 11 were women and 4 were men. The mean age was 21.20 years. There were not a statistically significant difference in L, ghrelin, A levels, and A/L ratio between the two groups. Adipokines may have a role in the pathogenesis of acne vulgaris. L, A, ghrelin, and insulin resistance may not participate in the responsible mechanisms in nonobese patients with moderate acne vulgaris. © The Author(s) 2016.

  8. The influence of aluminium availability on phosphate uptake in Phaseolus vulgaris L. and Phaseolus lunatus L.

    Science.gov (United States)

    Mimmo, Tanja; Sciortino, Marco; Ghizzi, Massimiliano; Gianquinto, Giorgio; Gessa, Carlo E

    2009-01-01

    Aluminium toxicity is one of the major limiting factors of crop productivity on acid soils. High levels of available aluminium in soil may induce phosphorus deficiency in plants. This study investigates the influence of Aluminium (Al) on the phosphate (P(i)) uptake of two Phaseolus species, Phaseolus vulgaris L. var. Red Kidney and Phaseolus lunatus L. The two bean species were treated first with solutions of Al at different concentrations (0, 25, 50 and 100microM, pH 4.50) and second with solutions of P(i) (150microM) at pH 4.50. The higher the Al concentration the higher the Al concentration sorbed but P. vulgaris L var. Red Kidney adsorbed significantly more Al than P. lunatus L. Both species released organic acids: P. vulgaris L var. Red Kidney released fumaric acid and P. lunatus L. fumaric and oxalic acids which could have hindered further Al uptake. The two bean species showed a sigmoid P(i) uptake trend but with two different mechanisms. P. vulgaris L var. Red Kidney showed a starting point of 3h whereas P. lunatus L. adsorbed P(i) immediately within the first minutes. In addition, P. vulgaris L var. Red Kidney presented significantly higher P(i) uptake (higher uptake rate 'k' and higher maximum adsorption 'a' of the kinetic uptake model). The Al treatments did not significantly influence P(i) uptake. Results suggest that P. lunatus L. might adopt an external Al detoxification mechanism by the release of oxalic acid. P. vulgaris L var. Red Kidney on the other hand seemed to adopt an internal detoxification mechanism even if the Al sorbed is poorly translocated into the shoots. More detailed studies will be necessary to better define Al tolerance and/or resistance of Phaseolus spp.

  9. PRESCRIPTION AUDIT OF ACNE VULGARIS IN SKIN OUTPATIENT DEPARTMENT OF A TERTIARY CARE TEACHING HOSPITAL

    Directory of Open Access Journals (Sweden)

    Vishal Prakash

    2014-10-01

    Full Text Available : OBJECTIVE: To evaluate prescribing pattern in acne vulgaris cases at a tertiary care teaching hospital in south India. METHODS: Prescriptions of 120 patients of acne vulgaris who attended Dermatology OPD of a tertiary care teaching hospital were selected for study and their drug data were analyzed. RESULTS: Topical Benzoyl peroxide, adapalene, ketoconazole were prescribed as monotherapy, while aloevera, liquid paraffin and white soft paraffin as polytherapy. Azithromycin, antibiotics, anti histaminics were prescribed as systemic monotherapy and polytherapy. Statistical analysis revealed p-value was > 0.05. CONCLUSIONS: Prescription patterns were in consensus with the general guidelines, with few changes, in the choice of established therapeutic agents.

  10. Antifungal activity of the essential oil of Thymus vulgaris L. and thymol on experimentally induced dermatomycoses.

    Science.gov (United States)

    Soković, M; Glamoclija, J; Cirić, A; Kataranovski, D; Marin, P D; Vukojević, J; Brkić, D

    2008-12-01

    The in vivo evaluation of the toxicological and antifungal activity of the essential oil of Thymus vulgaris L. and its main component thymol was made on 2-month-old male Wistar rats. We examined the therapeutic potency against experimentally induced dermatomycoses in rats, using the most frequent dermatomycetes, Trichophyton mentagrophytes, T. rubrum, and T. tonsurans. The therapeutic efficacy of a 1% solution of the essential oil of Thymus vulgaris and thymol as well as the commercial preparation bifonazole was evaluated. During the 37-day observation period the oil-treated animals were cured.

  11. Static and dynamic superheated water extraction of essential oil components from Thymus vulgaris L.

    Science.gov (United States)

    Dawidowicz, Andrzej L; Rado, Ewelina; Wianowska, Dorota

    2009-09-01

    Superheated water extraction (SWE) performed in both static and dynamic condition (S-SWE and D-SWE, respectively) was applied for the extraction of essential oil from Thymus vulgaris L. The influence of extraction pressure, temperature, time, and flow rate on the total yield of essential oil and the influence of extraction temperature on the extraction of some chosen components are discussed in the paper. The SWE extracts are related to PLE extracts with n-hexane and essential oil obtained by steam distillation. The superheated water extraction in dynamic condition seems to be a feasible option for the extraction of essential oil components from T. vulgaris L.

  12. Radiation-induced pemphigus vulgaris of the breast; Pemphigus vulgaire radio-induit du sein

    Energy Technology Data Exchange (ETDEWEB)

    Vigna-Taglianti, R.; Russi, E.G. [Department of radiotherapy, Santa Croce e Carle general hospital, Via M. Coppino, 12100 Cuneo (Italy); Denaro, N. [Oncology department, university of Messina, Via consolare Valeria no 1, 98100 Messina (Italy); Numico, G. [Department of medical oncology, U. Parini hospital, 11100 Aosta (Italy); Brizio, R. [Department of histopathology, Santa Croce e Carle general hospital, Via M. Coppino, 12100 Cuneo (Italy)

    2011-07-15

    Pemphigus vulgaris is a rare autoimmune muco-cutaneous bullous disease. Patients with a history of pemphigus vulgaris - who need radiotherapy - may show a long lasting bullous cutaneous manifestation, typical of pemphigus, within radiation fields. The literature describes fewer than 20 radio-induced cases. While systematic corticosteroid therapy has proven to be useful, topical treatment used in association with corticosteroid therapy is rarely described. To our knowledge the use of modern dressing products has never been described. We report our experience in a case in which modern dressing products were usefully associated to systemic therapy. (authors)

  13. A preliminary investigation of Nigerian Gmelina arborea and Bambusa vulgaris for pulp and paper production

    CSIR Research Space (South Africa)

    Azeez, MA

    2015-10-01

    Full Text Available 18(1): 65 - 78, 2016 DOI: 10.4067/S0718-221X2016005000007 ISSN impresa 0717-3644 ISSN online 0718-221X A PRELIMINARY INVESTIGATION OF NIGERIAN Gmelina arborea AND Bambusa vulgaris FOR PULP AND PAPER PRODUCTION... Mayowa Akeem Azeez1,♠, Jerome E. Andrew2, B. Bruce Sithole3 ABSTRACT Two common Nigerian-grown biomasses, gmelina (Gmelina arborea) and bamboo (Bambusaa vulgaris) have been pulped and their fibre characteristics and paper properties examined. The results...

  14. Effect of feeding Cuminum cyminum fruits, Thymus vulgaris leaves or their mixture to rats.

    Science.gov (United States)

    Haroun, E M; Mahmoud, O M; Adam, S E I

    2002-04-01

    Cuminum cyminum fruits and T. vulgaris leaves were fed to male Wistar rats at 2% or 10% of standard diet for 6 w. A mixture (5% +5%) of the 2 plants was also fed to rats for a similar period. Diets containing 2% C. cyminum fruits, 2% or 10% T. vulgaris leaves were not toxic to rats. Impairment of growth and enterohepatonephropathy were observed in the rats fed a diet containing 10% C. cyminum fruits. These changes were also seen in the rats fed the mixture of the 2 plants and were accompanied by leukopenia, anemia and increases in serum AST activity and urea and by decreased total protein and albumin levels.

  15. Bindi Tuberculosis - Lupus Vulgaris Associated with Bindi Use: A Case Report.

    Science.gov (United States)

    Mishra, Gyanshankar; Rathi, Sushil; Mulani, Jasmin

    2015-05-01

    Cutaneous lesions are relatively uncommon manifestations of tuberculosis (TB). A 40-year-old female presented with skin lesion over the forehead since two months. She used to apply bindi over the same area since past 25 years. Based on skin biopsy and other ancillary investigations, she was diagnosed as a case of lupus vulgaris and initiated on anti TB medications following which, the lesion regressed. Thus, we herein report the first case of cutaneous tuberculosis (lupus vulgaris) associated with long term use of bindi.

  16. Performance of conventional pcr for screening for strongylus vulgaris on horse farms

    DEFF Research Database (Denmark)

    Nielsen, Marianne K.; Wøhlk, Chamilla B.M.; Petersen, Stig L.

      Strongyle parasites are ubiquitous in grazing horses. Of these, the bloodworm Strongylus vulgaris is regarded most pathogenic. Increasing levels of anthelmintic resistance in strongyle parasites has lead to recommendations of decreased treatment intensities, and there is now a pronounced need...... for reliable tools for detection of parasite burdens in general and S. vulgaris in particular. The only method currently available is the larval culture, which is laborious and time-consuming, so veterinary practitioners most often pool samples from several horses together in one culture to save time...

  17. Effects of copper on reserve mobilization in embryo of Phaseolus vulgaris L.

    Science.gov (United States)

    Karmous, Inès; Bellani, Lorenza M; Chaoui, Abdelilah; El Ferjani, Ezzedine; Muccifora, Simonetta

    2015-07-01

    The present research reports a biochemical and micro-submicroscopic analysis of copper effect on reserve mobilization during germination of Phaseolus vulgaris L. var. soisson nain hatif seeds. Dry embryonic cells are rich in protein bodies and little starch grains. In Cu-treated embryos copper inhibited 50% of albumin and globulin mobilization after 72 h imbibition. The severe alterations in treated embryo cells, observed by electron microscope, were probably the cause of the inability to utilize the amino acids freed by protein mobilization and so possibly the cause of the inhibition of P. vulgaris embryonic axis elongation.

  18. Impact of light quality on biomass production and fatty acid content in the microalga Chlorella vulgaris.

    Science.gov (United States)

    Hultberg, Malin; Jönsson, Helene Larsson; Bergstrand, Karl-Johan; Carlsson, Anders S

    2014-05-01

    In this study, the green microalga Chlorella vulgaris was exposed to monochromatic light at six different wavelengths in order to study the effect on biomass productivity and fatty acid content. A significantly higher amount of biomass by produced in the treatments with yellow, red and white light compared with blue, green and purple light. There were also significant differences in total lipid content and fatty acid profile between the treatments. The green light regime gave the lowest concentration of lipids, but increased the concentration of polyunsaturated fatty acids. Thus it can be concluded that light quality significantly affects biomass productivity, total lipid concentration and fatty acid profile in the microalga C. vulgaris.

  19. TERMINALIA CHEBULA: A TREATMENT AGAINST PATHOGENIC PROTEUS VULGARIS STRAINS ASSOCIATED WITH URINARY TRACT INFECTION

    OpenAIRE

    Tariq. A. L; Reyaz. A. L

    2013-01-01

    Terminalia chebula was used to find out the new sort of treatment for the urinary tract infections caused by Proteus vulgaris. The causative agent was identified as Proteus vulgaris by staining and biochemical methods. It is responsible to cause urinary tract infection and most of strains show the resistance against the broad spectrum antibiotics: Ceftazidime (30μg), Ofloxacin (50μg), Norfloxacin (30μg), Tetracycline (30μg), Ampicillin (30μg), Chloramphenicol (25μg) and Gentamycin (20μg). The...

  20. Autoimmune bullous diseases with skin and eye involvement: Cicatricial pemphigoid, pemphigus vulgaris, and pemphigus paraneoplastica.

    Science.gov (United States)

    Broussard, Karen C; Leung, Theresa G; Moradi, Ahmadreza; Thorne, Jennifer E; Fine, Jo-David

    2016-01-01

    Autoimmune blistering diseases are a heterogeneous group of disorders that mostly affect the skin and mucous membranes. Occasionally, other organ systems may be involved, depending on the unique pathophysiology of each disease. Cicatricial pemphigoid, pemphigus vulgaris, and paraneoplastic pemphigus are distinct entities, but all have the potential to have cutaneous and ocular involvement. Awareness and early recognition of ocular involvement in these diseases is important given the increased risk for vision loss and blindness with delay in management. Several skin diseases may be associated with involvement of the external eye. The most common autoimmune diseases are cicatricial pemphigoid, pemphigus vulgaris, and paraneoplastic pemphigus.

  1. Current Pathophysiological Aspects and Therapeutic Modalities for Pemphigus Vulgaris : A Review

    Directory of Open Access Journals (Sweden)

    J Raviraj

    2007-01-01

    Full Text Available Pemphigus vulgaris (PV is an autoimmune disorder manifesting primarily as blisters involving the mucocutaneous systems. The current medical literature indicates many breakthroughs in the research of pathophysiology and treatment aspects of PV. This article tries to describe some of the novel aspects briefing the role of nondesmoglein antibodies and the role of TNF-alpha in the etiopathogenesis of pemphigus vulgaris and the role of newer therapeutic modalities like Rituximab, Etanercept, intravenous Immunoglobulins, cholinergic drugs, arid the like in the treatment of PV.

  2. Acne vulgaris: Perceptions and beliefs of Saudi adolescent males

    Science.gov (United States)

    Al-Natour, Sahar H.

    2017-01-01

    INTRODUCTION: Although acne vulgaris is common in adolescents, information on their understanding of acne is minimal. OBJECTIVES: To evaluate the perceptions and beliefs of Saudi youth on acne. MATERIALS AND METHODS: Three hundred twenty-nine male students (aged 13–22 years) from 6 secondary schools in the Eastern Saudi Arabia completed a self-reported questionnaire on knowledge, causation, exacerbating and relieving factors of acne. Data were analyzed by SPSS version 15.0. Results of subjects with acne, a family history of acne, and parents' educational levels were compared. Differences between the analyzed groups were assessed by a Chi-square test; p ≤ 0.05 was considered statistically significant. RESULTS: Over half (58.9%) of the participants considered acne a transient condition not requiring therapy. Only 13.1% knew that the proper treatment of acne could take a long time, even several years. Over half (52%) thought acne can be treated from the first or after few visits to the doctor. Popular sources of information were television/radio (47.7%), friends (45.6%), and the internet (38%). Only 23.4% indicated school as a source of knowledge. Reported causal factors included scratching (88.5%) and squeezing (82.1%) of pimples, poor hygiene (83.9%), poor dietary habits (71.5%), and stress (54.1%). Ameliorating factors included frequent washing of the face (52.9%), exercise (41.1%), sunbathing (24.1%), and drinking of mineral water (21%). The correlations of these facts are discussed. CONCLUSION: Results of this study point out that misconceptions of acne are widespread among Saudi youth. A health education program is needed to improve the understanding of the condition. PMID:28163574

  3. I know my neighbour: individual recognition in Octopus vulgaris.

    Directory of Open Access Journals (Sweden)

    Elena Tricarico

    Full Text Available BACKGROUND: Little is known about individual recognition (IR in octopuses, although they have been abundantly studied for their sophisticated behaviour and learning capacities. Indeed, the ability of octopuses to recognise conspecifics is suggested by a number of clues emerging from both laboratory studies (where they appear to form and maintain dominance hierarchies and field observations (octopuses of neighbouring dens display little agonism between each other. To fill this gap in knowledge, we investigated the behaviour of 24 size-matched pairs of Octopus vulgaris in laboratory conditions. METHODOLOGY/PRINCIPAL FINDINGS: The experimental design was composed of 3 phases: Phase 1 (acclimatization: 12 "sight-allowed" (and 12 "isolated" pairs were maintained for 3 days in contiguous tanks separated by a transparent (and opaque partition to allow (and block the vision of the conspecific; Phase 2 (cohabitation: members of each pair (both sight-allowed and isolated were transferred into an experimental tank and were allowed to interact for 15 min every day for 3 consecutive days; Phase 3 (test: each pair (both sight-allowed and isolated was subject to a switch of an octopus to form pairs composed of either familiar ("sham switches" or unfamiliar conspecifics ("real switches". Longer latencies (i.e. the time elapsed from the first interaction and fewer physical contacts in the familiar pairs as opposed to the unfamiliar pairs were used as proxies for recognition. CONCLUSIONS: Octopuses appear able to recognise conspecifics and to remember the individual previously met for at least one day. To the best of our knowledge, this is the first experimental study showing the occurrence of a form of IR in cephalopods. Future studies should clarify whether this is a "true" IR.

  4. Diversification and population structure in common beans (Phaseolus vulgaris L..

    Directory of Open Access Journals (Sweden)

    Matthew W Blair

    Full Text Available Wild accessions of crops and landraces are valuable genetic resources for plant breeding and for conserving alleles and gene combinations in planta. The primary genepool of cultivated common beans includes wild accessions of Phaseolus vulgaris. These are of the same species as the domesticates and therefore are easily crossable with cultivated accessions. Molecular marker assessment of wild beans and landraces is important for the proper utilization and conservation of these important genetic resources. The goal of this research was to evaluate a collection of wild beans with fluorescent microsatellite or simple sequence repeat markers and to determine the population structure in combination with cultivated beans of all known races. Marker diversity in terms of average number of alleles per marker was high (13 for the combination of 36 markers and 104 wild genotypes that was similar to the average of 14 alleles per marker found for the 606 cultivated genotypes. Diversity in wild beans appears to be somewhat higher than in cultivated beans on a per genotype basis. Five populations or genepools were identified in structure analysis of the wild beans corresponding to segments of the geographical range, including Mesoamerican (Mexican, Guatemalan, Colombian, Ecuadorian-northern Peruvian and Andean (Argentina, Bolivia and Southern Peru. The combined analysis of wild and cultivated accessions showed that the first and last of these genepools were related to the cultivated genepools of the same names and the penultimate was found to be distinct but not ancestral to the others. The Guatemalan genepool was very novel and perhaps related to cultivars of race Guatemala, while the Colombian population was also distinct. Results suggest geographic isolation, founder effects or natural selection could have created the different semi-discrete populations of wild beans and that multiple domestications and introgression were involved in creating the diversity of

  5. Importance of the dentist in early diagnosis of pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Thiago de Santana Santos

    2009-10-01

    Full Text Available The vulgar pemphigus is a chronic, rare, vesicle-bubble disease of autoimmune origin and with a possibility of following a dangerous clinical course when it is not diagnosed and treated in its initial stage. It usually affects people from 40 to 60 years old, being rare in children. In the majority of cases, oral manifestations are the first signs of the disease, so that dentists play an important rol in its early diagnosis. The authors present a case report of vulgar pemphigus in a 17 year-old patient, attended by the Bucco-Maxillo-Facial Surgery Service of the “Fundação de Beneficência Hospital de Cirurgia” in Aracaju-SE, Brazil. The patient was admitted with a complaint of the presence of numerous scattered painful ulcers in the mouth that had developed in approximately two months, and reported that at first, blisters that broke quickly appeared, leading to extremely painful ulcerations. Incisional biopsies were performed in the jugal mucosa and retromolar regions, and also a complete hemogram to discard the hypothesis of leukemia. In view of the clinical and histopathological findings, the final diagnosis of pemphigus vulgaris was made. Before receiving specialized treatment, the patient presented marked worsening of the clinical condition, with exacerbation of intraoral signs and symptoms and development of skin lesions around the body surface. The patient was hospitalized in the “Hospital Universitário da Universidade Federal de Sergipe” and treated with prednisone, cimetidine and nystatin, showing significant improvement of symptoms in approximately two weeks. At present, the patient is under the care of an interdisciplinary team that includes dermatologists and dentists.

  6. Mechanisms of Mitochondrial Damage in Keratinocytes by Pemphigus Vulgaris Antibodies*

    Science.gov (United States)

    Kalantari-Dehaghi, Mina; Chen, Yumay; Deng, Wu; Chernyavsky, Alex; Marchenko, Steve; Wang, Ping H.; Grando, Sergei A.

    2013-01-01

    The development of nonhormonal treatment of pemphigus vulgaris (PV) has been hampered by a lack of clear understanding of the mechanisms leading to keratinocyte (KC) detachment and death in pemphigus. In this study, we sought to identify changes in the vital mitochondrial functions in KCs treated with the sera from PV patients and healthy donors. PV sera significantly increased proton leakage from KCs, suggesting that PV IgGs increase production of reactive oxygen species. Indeed, measurement of intracellular reactive oxygen species production showed a drastic increase of cell staining in response to treatment by PV sera, which was confirmed by FACS analysis. Exposure of KCs to PV sera also caused dramatic changes in the mitochondrial membrane potential detected with the JC-1 dye. These changes can trigger the mitochondria-mediated intrinsic apoptosis. Although sera from different PV patients elicited unique patterns of mitochondrial damage, the mitochondria-protecting drugs nicotinamide (also called niacinamide), minocycline, and cyclosporine A exhibited a uniform protective effect. Their therapeutic activity was validated in the passive transfer model of PV in neonatal BALB/c mice. The highest efficacy of mitochondrial protection of the combination of these drugs found in mitochondrial assay was consistent with the ability of the same drug combination to abolish acantholysis in mouse skin. These findings provide a theoretical background for clinical reports of the efficacy of mitochondria-protecting drugs in PV patients. Pharmacological protection of mitochondria and/or compensation of an altered mitochondrial function may therefore become a novel approach to development of personalized nonhormonal therapies of patients with this potentially lethal autoimmune blistering disease. PMID:23599429

  7. Dissecting Phaseolus vulgaris innate immune system against Colletotrichum lindemuthianum infection.

    Science.gov (United States)

    Oblessuc, Paula Rodrigues; Borges, Aline; Chowdhury, Bablu; Caldas, Danielle Gregório Gomes; Tsai, Siu Mui; Camargo, Luis Eduardo Aranha; Melotto, Maeli

    2012-01-01

    The genus Colletotrichum is one of the most economically important plant pathogens, causing anthracnose on a wide range of crops including common beans (Phaseolus vulgaris L.). Crop yield can be dramatically decreased depending on the plant cultivar used and the environmental conditions. This study aimed to identify potential genetic components of the bean immune system to provide environmentally friendly control measures against this fungus. As the common bean is not amenable to reverse genetics to explore functionality and its genome is not fully curated, we used putative Arabidopsis orthologs of bean expressed sequence tag (EST) to perform bioinformatic analysis and experimental validation of gene expression to identify common bean genes regulated during the incompatible interaction with C. lindemuthianum. Similar to model pathosystems, Gene Ontology (GO) analysis indicated that hormone biosynthesis and signaling in common beans seem to be modulated by fungus infection. For instance, cytokinin and ethylene responses were up-regulated and jasmonic acid, gibberellin, and abscisic acid responses were down-regulated, indicating that these hormones may play a central role in this pathosystem. Importantly, we have identified putative bean gene orthologs of Arabidopsis genes involved in the plant immune system. Based on experimental validation of gene expression, we propose that hypersensitive reaction as part of effector-triggered immunity may operate, at least in part, by down-regulating genes, such as FLS2-like and MKK5-like, putative orthologs of the Arabidopsis genes involved in pathogen perception and downstream signaling. We have identified specific bean genes and uncovered metabolic processes and pathways that may be involved in the immune response against pathogens. Our transcriptome database is a rich resource for mining novel defense-related genes, which enabled us to develop a model of the molecular components of the bean innate immune system regulated upon

  8. Dissecting Phaseolus vulgaris innate immune system against Colletotrichum lindemuthianum infection.

    Directory of Open Access Journals (Sweden)

    Paula Rodrigues Oblessuc

    Full Text Available BACKGROUND: The genus Colletotrichum is one of the most economically important plant pathogens, causing anthracnose on a wide range of crops including common beans (Phaseolus vulgaris L.. Crop yield can be dramatically decreased depending on the plant cultivar used and the environmental conditions. This study aimed to identify potential genetic components of the bean immune system to provide environmentally friendly control measures against this fungus. METHODOLOGY AND PRINCIPAL FINDINGS: As the common bean is not amenable to reverse genetics to explore functionality and its genome is not fully curated, we used putative Arabidopsis orthologs of bean expressed sequence tag (EST to perform bioinformatic analysis and experimental validation of gene expression to identify common bean genes regulated during the incompatible interaction with C. lindemuthianum. Similar to model pathosystems, Gene Ontology (GO analysis indicated that hormone biosynthesis and signaling in common beans seem to be modulated by fungus infection. For instance, cytokinin and ethylene responses were up-regulated and jasmonic acid, gibberellin, and abscisic acid responses were down-regulated, indicating that these hormones may play a central role in this pathosystem. Importantly, we have identified putative bean gene orthologs of Arabidopsis genes involved in the plant immune system. Based on experimental validation of gene expression, we propose that hypersensitive reaction as part of effector-triggered immunity may operate, at least in part, by down-regulating genes, such as FLS2-like and MKK5-like, putative orthologs of the Arabidopsis genes involved in pathogen perception and downstream signaling. CONCLUSIONS/SIGNIFICANCE: We have identified specific bean genes and uncovered metabolic processes and pathways that may be involved in the immune response against pathogens. Our transcriptome database is a rich resource for mining novel defense-related genes, which enabled us to

  9. Mechanisms of mitochondrial damage in keratinocytes by pemphigus vulgaris antibodies.

    Science.gov (United States)

    Kalantari-Dehaghi, Mina; Chen, Yumay; Deng, Wu; Chernyavsky, Alex; Marchenko, Steve; Wang, Ping H; Grando, Sergei A

    2013-06-07

    The development of nonhormonal treatment of pemphigus vulgaris (PV) has been hampered by a lack of clear understanding of the mechanisms leading to keratinocyte (KC) detachment and death in pemphigus. In this study, we sought to identify changes in the vital mitochondrial functions in KCs treated with the sera from PV patients and healthy donors. PV sera significantly increased proton leakage from KCs, suggesting that PV IgGs increase production of reactive oxygen species. Indeed, measurement of intracellular reactive oxygen species production showed a drastic increase of cell staining in response to treatment by PV sera, which was confirmed by FACS analysis. Exposure of KCs to PV sera also caused dramatic changes in the mitochondrial membrane potential detected with the JC-1 dye. These changes can trigger the mitochondria-mediated intrinsic apoptosis. Although sera from different PV patients elicited unique patterns of mitochondrial damage, the mitochondria-protecting drugs nicotinamide (also called niacinamide), minocycline, and cyclosporine A exhibited a uniform protective effect. Their therapeutic activity was validated in the passive transfer model of PV in neonatal BALB/c mice. The highest efficacy of mitochondrial protection of the combination of these drugs found in mitochondrial assay was consistent with the ability of the same drug combination to abolish acantholysis in mouse skin. These findings provide a theoretical background for clinical reports of the efficacy of mitochondria-protecting drugs in PV patients. Pharmacological protection of mitochondria and/or compensation of an altered mitochondrial function may therefore become a novel approach to development of personalized nonhormonal therapies of patients with this potentially lethal autoimmune blistering disease.

  10. Autoantibodies other than Anti-desmogleins in Pemphigus Vulgaris Patients

    Science.gov (United States)

    Saleh, Marwah Adly; Salem, Hedayat; El Azizy, Hoda

    2017-01-01

    Background: Pemphigus vulgaris (PV) is an immunoglobulin G-mediated autoimmune bullous skin disease. Nonorgan-specific antibodies were detected in Tunisian and Brazilian pemphigus patients with different prevalence. Materials and Methods: Fifty PV patients and fifty controls were screened for antinuclear antibodies (ANAs), anti-smooth muscle antibodies (ASMAs), anti-parietal antibodies (APAs), anti-mitochondrial antibodies, and Anti-nuclear cytoplasmic antibodies (ANCA) by indirect immunofluorescence. Results: Thirty-nine patients were female and 11 were male. Fifteen patients did not receive treatment before while 35 patients were on systemic steroid treatment ± azathioprine. Twenty (40%) of the PV patients and 1 (2%) control had positive ANA. ANA was significantly higher in PV patients than controls, P < 0.0001. ASMAs were detected in 20 (40%) PV patients and none of the controls. ASMA was significantly higher in PV patients than controls, P < 0.0001. No significant difference was detected between treated and untreated regarding ANA, P - 0.11. However, there was a significant difference between treated and untreated regarding ASMA, P - 0.03. Six patients (12%) and none of the controls had positive APA. There was a significant difference between the patients and the controls in APA. P - 0.027. Conclusion: Egyptian PV patients showed more prevalent ANA, ASMA, and APA than normal controls. Follow-up of those patients is essential to detect the early development of concomitant autoimmune disease. Environmental factors might account for the variability of the nonorgan-specific antibodies among different populations. PMID:28216725

  11. Autoantibodies other than anti-desmogleins in pemphigus vulgaris patients

    Directory of Open Access Journals (Sweden)

    Marwah Adly Saleh

    2017-01-01

    Full Text Available Background: Pemphigus vulgaris (PV is an immunoglobulin G-mediated autoimmune bullous skin disease. Nonorgan-specific antibodies were detected in Tunisian and Brazilian pemphigus patients with different prevalence. Materials and Methods: Fifty PV patients and fifty controls were screened for antinuclear antibodies (ANAs, anti-smooth muscle antibodies (ASMAs, anti-parietal antibodies (APAs, anti-mitochondrial antibodies, and Anti-nuclear cytoplasmic antibodies (ANCA by indirect immunofluorescence. Results: Thirty-nine patients were female and 11 were male. Fifteen patients did not receive treatment before while 35 patients were on systemic steroid treatment ± azathioprine. Twenty (40% of the PV patients and 1 (2% control had positive ANA. ANA was significantly higher in PV patients than controls, P< 0.0001. ASMAs were detected in 20 (40% PV patients and none of the controls. ASMA was significantly higher in PV patients than controls, P< 0.0001. No significant difference was detected between treated and untreated regarding ANA, P - 0.11. However, there was a significant difference between treated and untreated regarding ASMA, P- 0.03. Six patients (12% and none of the controls had positive APA. There was a significant difference between the patients and the controls in APA. P- 0.027. Conclusion: Egyptian PV patients showed more prevalent ANA, ASMA, and APA than normal controls. Follow-up of those patients is essential to detect the early development of concomitant autoimmune disease. Environmental factors might account for the variability of the nonorgan-specific antibodies among different populations.

  12. Management of acne vulgaris: an evidence-based update.

    Science.gov (United States)

    Ingram, J R; Grindlay, D J C; Williams, H C

    2010-06-01

    This review summarizes clinically important findings from 3 systematic reviews, 1 updated guideline and a selection from the 62 randomized controlled trials (RCTs) published between February 2007 and January 2009 on the topic of acne vulgaris. Low glycaemic-load diets might reduce acne severity but this remains unproven. Written patient information leaflets have not been surpassed by other communication methods. New combination topical treatments have not shown convincing advantages over current combination products such as clindamycin/benzoyl peroxide. Topical dapsone is superior to placebo but has yet to be compared with standard topical treatments. Long-term topical tretinoin to prevent nonmelanoma skin cancer in elderly men was associated with higher all-cause mortality, but there is currently no evidence of increased mortality for topical retinoid use when treating acne. All oral tetracyclines have similar efficacy, yet minocycline is the most costly. Oral isotretinoin monotherapy remains the gold-standard treatment for severe acne. Flutamide plus the oral contraceptive pill is beneficial for acne associated with polycystic ovary syndrome. Photodynamic therapy, phototherapy and laser therapy cannot be recommended universally for acne until minimal postinflammatory pigmentation and longer-term benefit can be shown, especially with current high costs. Development of non-antibiotic therapies is preferable to minimize the risk of community antibiotic resistance. Future trials should use active comparators at optimum doses and avoid noninferiority comparisons unless appropriately powered. Trials need to shift from using multiple, unvalidated outcome measures to including patient-reported and quality-of-life outcomes, and all trials should be registered on a public clinical-trials database.

  13. Effect of the glycemic index of carbohydrates on Acne vulgaris.

    Science.gov (United States)

    Reynolds, Rebecca C; Lee, Stephen; Choi, James Y J; Atkinson, Fiona S; Stockmann, Karola S; Petocz, Peter; Brand-Miller, Jennie C

    2010-10-01

    Acne vulgaris may be improved by dietary factors that increase insulin sensitivity. We hypothesized that a low-glycemic index diet would improve facial acne severity and insulin sensitivity. Fifty-eight adolescent males (mean age ± standard deviation 16.5 ± 1.0 y and body mass index 23.1 ± 3.5 kg/m(2)) were alternately allocated to high or low glycemic index diets. Severity of inflammatory lesions on the face, insulin sensitivity (homeostasis modeling assessment of insulin resistance), androgens and insulin-like growth factor-1 and its binding proteins were assessed at baseline and at eight weeks, a period corresponding to the school term. Forty-three subjects (n = 23 low glycemic index and n = 20 high glycemic index) completed the study. Diets differed significantly in glycemic index (mean ± standard error of the mean, low glycemic index 51 ± 1 vs. high glycemic index 61 ± 2, p = 0.0002), but not in macronutrient distribution or fiber content. Facial acne improved on both diets (low glycemic index -26 ± 6%, p = 0.0004 and high glycemic index -16 ± 7%, p = 0.01), but differences between diets did not reach significance. Change in insulin sensitivity was not different between diets (low glycemic index 0.2 ± 0.1 and high glycemic index 0.1 ± 0.1, p = 0.60) and did not correlate with change in acne severity (Pearson correlation r = -0.196, p = 0.244). Longer time frames, greater reductions in glycemic load or/and weight loss may be necessary to detect improvements in acne among adolescent boys.

  14. GAMBARAN KARAKTERISTIK KASUS PSORIASIS VULGARIS DI POLIKLINIK PENYAKIT KULIT DAN KELAMIN RSUD KABUPATEN BULELENG TAHUN 2012-2013

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    Komang Aditya Yudistira

    2014-12-01

    Full Text Available Psoriasis merupakan penyakit kulit kronis yang sering mengalami kekambuhan. Penyakit psoriasis tidak segera mengancam nyawa akan tetapi sangat mempengaruhi kualitas hidup penderitanya. Tujuan dari studi ini adalah untuk mengetahui profil dari pasien psoriasis vulgaris dan gambaran karakteristik pasien dengan psoriasis vulgaris. Penelitian ini adalah penelitian deskriptif dengan mengambil data sekunder pasien psoriasis vulgaris yang berobat ke poliklinik kulit dan kelamin RSUD Buleleng pada tahun 2012 sampai 2013. Sampel penelitian ini adalah pasien dengan psoriasis vulgaris yang berobat ke poliklinik kulit dan kelamin RSUD Buleleng. Dari hasil penelitian ini didapatkan sebanyak 54 pasien mengalami penyakit psoriasis vulgaris pada tahun 2012 dan sebanyak 50 pasien mengalami penyakit psoriasis vulgaris pada tahun 2013. Dari kedua tahun ini hampir sebagian besar merupakan kasus lama, persebaran kelompok usia hampir sama di semua kelompok usia dan jenis kelamin. Dari karakteristik pasien, sebanyak 74,1% dengan BMI normal pada tahun 2012 dan 54% pasien dengan BMI normal pada tahun 2013. 25,9% pasien yang memiliki kebiasaan merokok pada tahun 2012 dan 28% pasien di tahun 2013. Sebanyak 16,7% pasien memiliki kebiasaan minum alkohol pada tahun 2012 dan 24% pasien di tahun 2013. Untuk riwayat penyakit lain pada pasien psoriasis vulgaris, 24% pasien dengan diabetes mellitus di tahun 2012 dan 20% pasien di tahun 2013,  5% pasien dengan rheumatoid artritis di tahun 2012 dan 4% pasien di tahun 2013.

  15. In Vitro Inhibitory Effect of Berberis vulgaris (Berberidaceae and Its Main Component, Berberine against Different Leishmania Species.

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    Hossein Mahmoudvand

    2014-03-01

    Full Text Available Leishmaniasis has been identified as a major public health problem in tropical and sub-tropical countries. The present study was aimed to investigate antileishmanial effects of various extracts of Berberis vulgaris also its active compoenent, berberine against Leishmania tropica and L. infantum species on in vitro experiments.In this study in vitro antileishmanial activity of various extracts of B. vulgaris also its active compoenent, berberine against promastigote and amastigote stages of L. tropica and L. infantum was evaluated, using MTT assay and in a macrophage model, respectively. Furthermore, infectivity rate and cytotoxicity effects of B. vulgaris and berberine in murine macrophage cells were investigated.The findings of optical density (OD and IC50 indicated that B. vulgaris particulary berberine significantly (P<0.05 inhibited the growth rate of promastigote stage of L.tropica and L.infantum in comparison to meglumine antimoniate (MA. In addition, B. vulgaris and berberine significantly (P<0.05 decreased the mean number of amastigotes in each macrophage as compared with positive control. In the evaluation of cytotoxicity effects, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages. Results also showed that when parasites were pre-incubated with B. vulgaris their ability to infect murine macrophages was significantly decreased.B.vulgaris particularly berberine exhibited potent in vitro leishmanicidal effects against L. tropica and L.infantum. Further works are required to evaluate the antileishmanial effects of B.vulgaris on Leishmania species using clinical settings.

  16. Comparison between the efficacy of metronidazole vaginal gel and Berberis vulgaris (Berberis vulgaris) combined with metronidazole gel alone in the treatment of bacterial vaginosis

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    Masoudi, Mansoure; Kopaei, Mahmoud Rafieian; Miraj, Sepideh

    2016-01-01

    Background Bacterial vaginosis is one of the most prevalent complications among reproductive-aged women. Antibacterial and antifungal effects of Berberis vulgaris have been demonstrated in vitro and in vivo. Objectives This study aimed to compare the therapeutic effects of the vaginal gel of Berberis vulgaris 5% (in metronidazole base) with metronidazole vaginal gel 0.75% on bacterial vaginosis on 80 patients referred to the Hajar Hospital from January 2012 to April 2013. Methods This study was a randomized clinical trial research on 80 women affected by bacterial vaginosis, who were randomly divided into two groups of 40 participants. Diagnostic criteria were Amsel’s criteria and Gram stain. Berberis vulgaris 5% (in metronidazole gel base) or metronidazole vaginal gel for five-night usage was prescribed to each group, and after two to seven days therapeutic effects and Amsel criteria were assessed. Data analysis was performed by SPSS 16 using Student t-test, chi-square, and ANOVA tests. Results Findings of the study showed a statistically significant difference with regard to treatment response between the study groups (pvaginosis therapy. Clinical trial registration The trial was registered at the Iranian Registry of Clinical Trials (http://www.irct.ir) with the IRCT ID: IRCT201411102085N13. Funding Shahrekord University of Medical Sciences supported this research. PMID:27757195

  17. Comparison between the efficacy of metronidazole vaginal gel and Berberis vulgaris (Berberis vulgaris) combined with metronidazole gel alone in the treatment of bacterial vaginosis.

    Science.gov (United States)

    Masoudi, Mansoure; Kopaei, Mahmoud Rafieian; Miraj, Sepideh

    2016-08-01

    Bacterial vaginosis is one of the most prevalent complications among reproductive-aged women. Antibacterial and antifungal effects of Berberis vulgaris have been demonstrated in vitro and in vivo. This study aimed to compare the therapeutic effects of the vaginal gel of Berberis vulgaris 5% (in metronidazole base) with metronidazole vaginal gel 0.75% on bacterial vaginosis on 80 patients referred to the Hajar Hospital from January 2012 to April 2013. This study was a randomized clinical trial research on 80 women affected by bacterial vaginosis, who were randomly divided into two groups of 40 participants. Diagnostic criteria were Amsel's criteria and Gram stain. Berberis vulgaris 5% (in metronidazole gel base) or metronidazole vaginal gel for five-night usage was prescribed to each group, and after two to seven days therapeutic effects and Amsel criteria were assessed. Data analysis was performed by SPSS 16 using Student t-test, chi-square, and ANOVA tests. Findings of the study showed a statistically significant difference with regard to treatment response between the study groups (pbacterial vaginosis therapy. The trial was registered at the Iranian Registry of Clinical Trials (http://www.irct.ir) with the IRCT ID: IRCT201411102085N13. Shahrekord University of Medical Sciences supported this research.

  18. Phytotoxicity Assessment of Certain Phytochemical Products Containing Pyrrolizidine Alkaloids

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    Cristina Șeremet Oana

    2013-10-01

    Full Text Available Introduction: Tussilago farfara (coltsfoot, Petasites hybridus (common butterbur, Senecio vernalis (eastern groundsel and Symphytum officinale (comfrey are species traditionally used in phytotherapy that besides the therapeutic compounds contain toxic pyrrolizidine alkaloids (PAs. The aim of the paper is to determine the total PAs content and the phytotoxicity of the above species. Material and methods: The quantitative determination of pyrrolizidine alkaloids is based on the stoichiometric reaction of protonated alkaloids with methyl orange. In acidic conditions the dye is released from the complex and its color is assessed spectrophotometrically using a linear regression curve of senecionine as a standard. The phytotoxicity was assessed by Triticum bioassay that studies the effect of the extracts (0.001-5.00%, w/v upon root elongation (inhibitory concentration - IC50 and on the karyokinetic film. Results: The highest amount of total PAs was found in Senecio vernalis (654.8 ± 35.96 μg/g dry plant and the lowest in Petasites hybridus. The lowest IC50 was found for Tussilago farfara followed by Petasites hybridus, Senecio vernalis, and Symphytum officinale. The results were supported by microscopic examination. Conclusions: The results of the spectrophotometric assay are consistent with the ones found in the literature. All extracts inhibited the elongation of the main root of wheat caryopses, however, no correlation between phytotoxicity and the PAs concentration could be emphasized

  19. Genetic diversity analysis with ISSR PCR on green algae Chlorella vulgaris and Chlorella pyrenoidosa

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    Shen, Songdong

    2008-11-01

    In the present study, genetic polymorphism and diversity in unicellular clones of Chlorella vulgaris Beijerinck and Chlorella pyrenoidosa Chick were studied with Inter Simple Sequence Repeats PCR (ISSR PCR). Samples including four clones of C. vulgaris and three clones of C. pyrenoidosa were purified by single-clone-choice method. For four C. vulgaris unicellular clones, the total number of the bands scored for 18 primers was 298; and the number of the polymorphic bands was 118, of which 39.6% were polymorphic. The size of PCR products ranged from 200 to 2 500 bp. The total number of bands scored for 18 primers, the number of polymorphic bands and the percentage of three C. pyrenoidosa unicellular clones was 194.83 and 30.8%, respectively. POPGENE analysis show that the average Nei genetic diversity (h*) and Shannon index of diversity (I*) in the four C. vulgaris unicellular clones was 0.2181 and 0.3208, respectively, which is slightly higher than those of the three C. pyrenoidosa unicellular clones (0.190 3 and 0.274 8), which agreed with the percentage of polymorphic bands in the mixed samples of the two species. The results suggest that ISSR is a useful method to Chlorella for intraspecies genetic analysis.

  20. Inhibitory effects of silver nanoparticles in two green algae, Chlorella vulgaris and Dunaliella tertiolecta.

    Science.gov (United States)

    Oukarroum, Abdallah; Bras, Sébastien; Perreault, François; Popovic, Radovan

    2012-04-01

    Freshwater microalga Chlorella vulgaris and marine microalga Dunaliella tertiolecta were used to investigate toxic effects induced by 50 nm silver nanoparticles (AgNPs). To induce AgNPs effect, we exposed Chlorella vulgaris and Dunaliella tertiolecta for 24h to 0-10 mg/L. We showed that growth media had different effects in AgNPs agglomerates' formation. Cellular viability, reactive oxygen species (ROS) formation and lipids peroxidation were employed to assess the toxic effects of AgNPs. AgNPs were able to interact directly with the Chlorella vulgaris cells surface and large aggregates were observed. AgNPs have a negative effect on Chlorella vulgaris and Dunaliella tertiolecta, as manifested by a strong decrease in chlorophyll content, viable algal cells, increased ROS formation and lipids peroxidation. The variability in sensitivity of both algae towards AgNPs was observed. We conclude that AgNPs have a negative effect on aquatic algae and these alterations might have serious consequences on structure and function of aquatic plant communities.