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Sample records for grandis hymenoptera sphecidae

  1. Primer registro del género Mellinus (Hymenoptera: Sphecidae en Colombia

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    Fernández C. Fernando

    1999-06-01

    Full Text Available Uno de los taxones menos conocidos de Sphecidae (Hymenoptera: Apoidea es Mellinus Fabricius, género que en el pasado llegó a tener categoría de subfamilia (Bohart & Menke 1976, pero que ahora comprende una tribu, Mellinini, dentro de la subfamilia Nyssoninae (Menke & Fernández 1996. Al parecer,  las hembras de este género capturan moscas del estiércol cerca a excrementos de mamíferos en el campo, y hacen sus nidos en el suelo (Evans 1989.

  2. Parasitismo entre especies (Diptera, Hymenoptera en los nidos de Stictia signata (Hymenoptera: Sphecidae

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    Julio A. Genaro

    1999-09-01

    Full Text Available S. signata es una de las avispas de la arena más frecuentemente observada en los cayos y las costas de Cuba. Las hembras construyen los nidos en la arena y los abastecen con moscas, para alimentar a la descendencia. Se describe la conducta de dos especies: Liohippelates n. sp. circa collusor (Diptera: Chloropidae y Hexacola sp. (Hymenoptera: Eucoilidae para penetrar al interior de los nidos de S. signata. Las observaciones se efectuaron durante 1989 hasta 1991, en playa Caimito, sur de la provincia de La Habana, Cuba. Liohippelates cleptoparasitó el 100% de los nidos. Sus larvas necrófagas se alimentaron de los restos de las presas dejadas por la larva de S. signata, sin afectarla. Sólo en un caso la larva mostró signos de mortalidad, porque además del número alto de cleptoparásitos inmaduros, habían 53 moscas adultas alimentándose de los fluidos corporales de las presas. Hexacola sp. fue un parasitoide de las larvas de Liohippelates, en el interior de las celdillas. A pesar del elevado cleptoparasitismo, la población del esfécido se mantuvo elevada durante los años de observación.Stictia signata is one of the most frequently observed sand wasps in the Cuban keys and coasts. Females build their nests in the sand and supply them with flies to feed offspring. Here, I describe the behavior of two species, Liohippelates n. sp. near collusor (Diptera: Chloropidae and Hexacola sp. (Hymenoptera: Eucoilidae, which enter the nests of S. signata. The observations were carried out from 1989 through 1991 in Caimito beach, Southern Havana province, Cuba. Liohippelates inhabited 100% of the nests. Its necrofagous larvae fed on the remnants of prey left by the larva of S. signata, without affecting the larva. Only in one case did the larva show signs of mortality because, apart from the high number of immature cleptoparasites, there were 53 adult flies feeding on prey body fluids. Hexacola sp. parasitized the larvae of Liohippelates within the

  3. Competition between Catolaccus grandis (Hymenoptera: Pteromalidae and Bracon vulgaris (Hymenoptera: Braconidae, parasitoids of the Boll Weevil

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    Francisco de Sousa Ramalho

    2007-05-01

    Full Text Available The competition between populations of the parasitoids C. grandis and B. vulgaris was studied using larvae of Euscepes postfasciatus (Fairmaire as an alternative host. A series of biological parameters was observed and related to the competitive abilities of both parasitoid species. They were capable of colonizing and maintaining their populations regardless of host location. The population growth of C. grandis and B. vulgaris, based on fecundity was not affected by the competition. The parasitism and survivorship to the adult stage were affected by competition, except when the host was located at the bottom of the rearing cage. C. grandis performed better than B. vulgaris independently of the competition and host location, but it did not exclude the other species.Catolaccus grandis (Burks e Bracon vulgaris Ashmead são os principais parasitóides do bicudo-do-algodoeiro Anthonomus grandis Boheman no Nordeste do Brasil. É importante que se determinem as interações entre esses parasitóides e o seu efeito em programas de controle biológico dessa praga com os mesmos. A competição entre os parasitóides C. grandis e B. vulgaris foi estudada, utilizando-se larvas de Euscepes postfasciatus Fairmaire como hospedeiro alternativo. A fecundidade de C. grandis e B. vulgaris baseada na produção de ovos, não foi afetada pela competição, mas o parasitismo e a produção de adultos desses parasitóides foram afetados pela competição, exceto quando o hospedeiro se encontrava na base da caixa de criação. Independentemente da competição e da localização do hospedeiro, C. grandis apresentou melhor desempenho que B. vulgaris, mas não excluiu as populações da outra espécie de parasitóide.

  4. Ultrastructure of the silk glands in three adult females of sphecid wasps of the genus Microstigmus (Hymenoptera: Pemphredoninae Ultraestructura de las glándulas de seda en hembras adultas de tres especies de avispas del género Microstigmus (Hymenoptera: Sphecidae: Pemphredoninae

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    JOSÉ EDUARDO SERRÃO

    2005-03-01

    Full Text Available The ultrastructure of silk glands in adult females of sphecid wasps is described. Individual glands are scattered in the more posterior portion of the metasoma being each gland formed by an enlarged secretory unity with one conducting canal. Each secretory unit is a gland cell containing a large lumen and numerous secretory granules, which are delimited by membrane and filled with middle electron dense content of homogeneous aspect, which are exocytosed into the extra cellular lumen. Cell cytoplasm is filled with rough endoplasmic reticulum, polyribosomes and Golgi apparatuses profiles. The basal plasma membrane has many short infoldings and is lined by a thin basement membrane. The apical membrane is invaginated to form the boundary of the lumen, containing scattered short microvilli. The cells are mononucleated and the nucleus is pleomorphic containing disperse chromatin. The extracellular lumen is separated of the receiving canal by a convoluted sponge-like wall forming many villi, which in their tip are lined by a thin single layered cuticle. In the basal region of the villi a cuticular covering is lacking. In the conducting canal secretion acquire two different electron densities. A thin cuticle lines the lumen of conducting canal and the cell cytoplasm is scarce and the organelles are represented for few rough endoplasmic reticulum and polyribosomes. These results are discussed in relation to function and origin of silk gland in adult SphecidaeSe describe la ultraestructura de las glándulas de la seda en tres hembras adultas de avispas Sphecidae del género Microstigmus. Las glándulas individualmente se dispersan en la porción más posterior del metasoma, y cada una de ellas está formada por una unidad secretora grande y un canal que conduce la secreción. Cada célula de la unidad secretora contiene un gran lumen y numerosos gránulos secretores, los que están delimitados por una membrana y repletos de un contenido denso a los

  5. Lethal and sublethal effects of selected insecticides and an insect growth regulator on the boll weevil (Coleoptera: Curculionidae) ectoparasitoid Catolaccus grandis (Hymenoptera: Pteromalidae).

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    Elzen, G W; Maldonado, S N; Rojas, M G

    2000-04-01

    A laboratory culture of Catolaccus grandis (Burks), an ectoparasitoid of the boll weevil, Anthonomus grandis grandis Boheman, was exposed to lethal and sublethal doses of insecticides and an insect growth regulator using a spray chamber bioassay. Materials tested were azinphos-methyl, endosulfan, fipronil, malathion, cyfluthrin, dimethoate, spinosad, methyl parathion, acephate, oxamyl, and tebufenozide. At full rates, spinosad was significantly less toxic to female C. grandis than other treatments except endosulfan. Fipronil and malathion were significantly more toxic to females than other treatments. Most of the chemicals tested were highly toxic to male C. grandis; spinosad was least toxic. At reduced rates, most of 4 selected chemicals tested were low in toxicity to C. grandis; however, a reduced rate of malathion was significantly more toxic to females than other treatments. No C. grandis pupae developed from parasitism during a 24-h treatment period with malathion or spinosad. The sex ratio of progeny from sprayed adults appeared to be unaffected by the treatments.

  6. FIRST RECORDS OF THE INVASIVE AMERICAN WASP ISODONTIA MEXICANA (HYMENOPTERA: SPHECIDAE) IN SERBIA

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    Ćetković, Aleksandar; Čubrilović, Branka; Plećaš, Milan; Popović, Anđelka; Savić, Dragiša; Stanisavljević, Ljubiša

    2012-01-01

    The first verified occurrences of the invasive American sphecid wasp, Isodontia mexicana (Saussure, 1867), in Serbia are reported. It was first collected from a trap-nest sample in the northern surroundings of Belgrade in 2010. During 2012, we recorded well-established, abundant populations in the central city area of Belgrade, its surroundings, and also at a single site on the Fruška Gora Mt. We briefly review the circumstances and course of its recent eastward and northeastward spreading in...

  7. Hymenoptera of Niğde province: Studies on Sphecidae fauna

    African Journals Online (AJOL)

    USER

    12 Tem 2010 ... Distribution in the world: North Mediterranean Region,. Turkey, South West Asia, Turkmenistan (Bohart and. Menke, 1976). Distribution in Turkey: Ankara, Erzurum (De Beaumont,. 1967). Sphex (Prionyx) viduatus (Christ, 1791). Analyzed materials: Central District: Tepeköy, 1339 m,. 18.08.07 (1♀). Niğde: ...

  8. Natural and synthetic oviposition stimulants forCatolaccus grandis (Burks) females.

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    Guerra, A A; Martinez, S; Sonia Del Rio, H

    1994-07-01

    Oviposition behavior was elicited fromCatolaccus grandis (Burks) (Hymenoptera: Pteromalidae) females, an ectoparasitoid of the boll weevil,Anthonomus grandis Boheman (Coleoptera: Curculionidae), by smears of freshly cut cotton bolls or smears of extracts prepared with boll weevil damaged or undamaged cotton boll tissues. Oviposition behavior was also elicited fromC. grandis females by smears made withn-pentane,n-hexane,n-heptane, and isooctane. This is the first report of oviposition behavior elicited for any parasitoid by these short-chain saturated hydrocarbons (alkanes), introducing a new concept on the chemical mediation of parasitoid behavior during host selection. Oviposition behavior was also elicited fromC. grandis females by volatiles emanating from an artificial diet devoid of insect components that was specifically developed for the in vitro rearing of ectoparasitoids. The possible use of a synergistic combination ofn-hexane and diet to optimize the mechanized production of noncontaminated eggs is also discussed.

  9. Hea õpetamise grandi saajad

    Index Scriptorium Estoniae

    2016-01-01

    Õpetamise uurimist alustavad grandi toel Andrus Org, Riina Oruaas, Natalja Zagura (humanitaar- ja kunstide valdkond), Stefano Braghiroli, Aet Kiisla, Tiiu Taur (sotsiaalteadused), Marje Oona, Oivi Uibo, Daisy Volmer (meditsiinitead.), Svetlana Ganina, Natalja Lepik ja Raivo Raid (loodus- ja täppistead.)

  10. Avaliação comparativa de iscas atrativas a partir da riqueza de espécies de formigas (Hymenoptera: Formicidae numa floresta de Eucalyptus grandis, em Santa Maria, Rio Grande do Sul, Brasil

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    Jardel Boscardin

    2011-10-01

    Full Text Available O presente estudo objetivou avaliar diferentes iscas atrativas, buscando eficiência quanto à riqueza de espécies de formigas epigéicas, potenciais bioindicadores da qualidade ambiental, em uma área antropizada, com Eucalyptus grandis Hill ex. Maiden (Myrtaceae, com 16 anos, e aproximadamente cinco hectares, localizada no campus da Universidade Federal de Santa Maria, em Santa Maria, Rio Grande do Sul. As coletas foram realizadas utilizando-se cinco tipos de iscas: sardinha com óleo (SO, sardinha com tomate (ST, patê de frango (FR, patê de fígado de frango (FG e atum sólido (AT distribuídas aleatoriamente na área, em intervalos de cinco metros, com cinco repetições, totalizando 25 unidades experimentais, amostradas a cada estação do ano, de novembro de 2007 a agosto de 2008. Após 60 minutos de exposição, todo o material presente sobre a armadilha foi recolhido e acondicionado em recipientes com álcool 70 % e em seguida, encaminhado ao laboratório de entomologia para separação em morfo-espécies para posterior identificação. Coletaram-se 3072 indivíduos, distribuídos em 14 espécies pertencentes a oito gêneros e quatro subfamílias. Não houve diferença estatística significativa entre as riquezas médias observadas de espécies nas iscas (DMS = 2,3252 ; g.l. = 19, p > 0,05. A isca constituída de sardinha com óleo apresentou maior riqueza média observada de espécies (SM = 5,75, porém a isca a base de patê de fígado de frango apresentou maior riqueza de espécies (S = 13, sendo que, Pseudomyrmex termitarius ocorreu somente nestas duas. Das espécies amostradas, nenhuma apresentou preferência por uma única isca atrativa específica, espécies de Brachymyrmex, Camponotus, Pheidole, Wasmannia e Solenopsis foram comuns nas cinco iscas. Portanto, no momento da escolha de uma das iscas avaliadas para a realização da amostragem de formigas epigéicas, sugere-se ponderar, além da riqueza específica, a não- presen

  11. First records of the American wasps Sceliphron caementarium (Drury, 1770 and Isodontia mexicana (de Saussure, 1867 (Hymenoptera: Sphecidae from Bulgaria

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    DENIS GRADINAROV

    2017-11-01

    Full Text Available Two alien sphecid species are reported for the first time for Bulgaria. Nearctic species Sceliphron caementarium (Drury, 1770 and Isodontia mexicana (de Saussure, 1867 were found in Sofia. The first species is collected as well from the Bulgarian Black Sea Coast, near Varna.

  12. Venom and Dufour's glands of the emerald cockroach wasp Ampulex compressa (Insecta, Hymenoptera, Sphecidae): structural and biochemical aspects.

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    Gnatzy, Werner; Michels, Jan; Volknandt, Walter; Goller, Stephan; Schulz, Stefan

    2015-09-01

    The digger wasp species Ampulex compressa produces its venom in two branched gland tubules. They terminate in a short common duct, which is bifurcated at its proximal end. One leg is linked with the venom reservoir, the other one extends to the ductus venatus. Each venom gland tubule possesses, over its entire length, a cuticle-lined central duct. Around this duct densely packed class 3 gland units each composed of a secretory cell and a canal cell are arranged. The position of their nuclei was demonstrated by DAPI staining. The brush border of the secretory cells surrounds the coiled end-apparatus. Venom is stored in a bladder like reservoir, which is surrounded by a thin reticulated layer of muscle fibres. The reservoir as a whole is lined with class 3 gland units. The tubiform Dufour's gland has a length of about 350 μm (∅ 125 μm) only and is surrounded by a network of pronounced striated muscle fibres. The glandular epithelium is mono-layered belonging to the class 1 type of insect epidermal glands. The gland cells are characterized by conspicuous lipid vesicles. Secretion of material via the gland cuticle into the gland lumen is apparent. Analysis of the polypeptide composition demonstrated that the free gland tubules and the venom reservoir contain numerous proteins ranging from 3.4 to 200 kDa. The polypeptide composition of the Dufour's gland is completely different and contains no lectin-binding glycoproteins, whereas a dominant component of the venom droplets is a glycoprotein of about 80 kDa. Comparison of the venom reservoir contents with the polypeptide pattern of venom droplets revealed that all of the major proteinaceous constituents are secreted. The secreted venom contains exclusively proteins present in the soluble contents of the venom gland. The most abundant compound class in the Dufour's gland consisted of n-alkanes followed by monomethyl-branched alkanes and alkadienes. Heptacosane was the most abundant n-alkane. Furthermore, a single volatile compound, 2-methylpentan-3-one, was identified in various concentrations in the lipid extract of the Dufour's gland. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Hymenoptera: Formicidae

    African Journals Online (AJOL)

    1990-05-15

    May 15, 1990 ... Supplemental Library User's Guide 1983 Edition. pp. 131-144. (Ed.) S. Ioyner. SAS Institute Incorporated, Cary,. North Carolina. CORBARA, B., LACHAUD, I. & FRESNEAU, D. 1989. Individual variability, social structure and division of labour in the ponerine ant, Ectatommo. ruidum Roger (Hymenoptera,.

  14. (TECTONA GRANDIS LEAF POWDER

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    Yash Mishra

    2015-01-01

    Full Text Available In this study, the adsorption potential of Teak (Tectona grandis leaf powder (TLP toremove Methylene blue (MB and Malachite Green (MG dye molecules from aqueoussolution was investigated. Batch experiments were conducted to evaluate the influenceof operational parameters such as, pH (2−9, adsorbent dosage (1−7 g/L, contact time(15−150 minutes and initial dye concentration (20−120 mg/L at stirring speed of 150rpm for the adsorption of MB and MG on TLP. Maximum removal efficiency of 98.4%and 95.1% was achieved for MB and MG dye, respectively. The experimentalequilibrium data were analysed using Langmuir, Freundlich and Temkin isothermmodels and it was found that, it fitted well to the Freundlich isotherm model. Thesurface structure and morphology of the adsorbent was characterized using scanningelectron microscopy (SEM and the presence of functional groups and its interactionwith the dye molecules were analysed using Fourier transform infrared spectroscopy(FTIR. Based on the investigation, it has been demonstrated that the teak leaf powderhas good potential for effective adsorption of methylene blue and malachite green dye.

  15. Parasitoids of boll weevil Anthonomus grandis and resident predators in kaolin-treated cotton

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    Roberta Leme Santos

    2013-12-01

    Full Text Available Simultaneous use of control methods is essential to reach success in managing arthropod pests. The current study investigated the effect of kaolin application on resident predators in the cotton plant canopy and parasitism of boll weevil on abscised squares in the field, and parasitism of boll weevil in the laboratory. Predators Araneae, Formicidae, Chrysopidae, and Coccinellidae showed similar seasonal densities for kaolin-treated and untreated cotton fields as well as the emergence rate of the parasitoids Bracon vulgaris Ashmead (Hymenoptera: Braconidae and Catolaccus grandis Burks (Hymenoptera: Pteromalidae from abscised field-collected structures. Under laboratory conditions, the parasitism of boll weevil larvae infesting squares was similar when treated and untreated squares with kaolin were offered to the parasitoid under free choice test. Therefore, the results show that spraying cotton fields with kaolin does not affect the natural biological control by parasitoids of boll weevil and pink bollworm and resident predators naturally occurring in cotton fields.

  16. Contenido de los nutrientes básicos en Catolaccus grandis Burks criados sobre larvas del picudo del algodon Basic nutrients content of Catolaccus grandis Burks reared in cotton boll weevil larvae

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    LÚCIA HELENA AVELINO ARAUJO

    2000-09-01

    Full Text Available El objetivo del trabajo fue determinar los niveles de carbohidratos, proteínas solubles y aminoácidos libres de larvas, pupas hembras y adultos hembras de Catolaccus grandis (Burks (Hymenoptera: Pteromalidae criados sobre larvas del picudo del algodón envenenadas por hembras del parasitoide y por larvas de primer instar del parasitoide. Esto estudio fue conducido en la Unidade de Investigación de Control Biologico de Plagas del Departamento de Agricultura de los Estados Unidos de la América, en Weslaco, Texas. Las 20 muestras de cada uno de los tres estados de desarrollo: tercer instar larval, pupas hembra y adultos hembra del parasitoide C. grandis, fueron separadas y pesadas individualmente y se cuantió el contenido de carbohidratos totales, proteínas solubles totales y aminoácidos libres criados en diferentes sustratos. Los resultados obtenidos confirman la existencia de patrones metabólicos significativamente distintos de estos nutrientes básicos.The aim of this work was to determine the levels of carbohydrate, soluble proteins and free amino acids of larvae, pupae and adult females Catolaccus grandis Burks (Hymenoptera: Pteromalidae which were reared in cotton (Gossypium hirsutum L. r. latifolium Hutch boll weevil (Anthonomus grandis larvae venomized by ectoparasitoid of female and 1st instar ectoparasitoid larvae. This study was carried out at the Biological Control of Pests Research Unit, Weslaco, Texas. The twenty samples of each one of three stages of development: 3rd instar larval, female pupae and female adult of parasitoid C. grandis were separated and individually weighted, and levels of carbohydrates, proteins and amino acids were quantified when reared in different substrates. The results confirmed the existence of metabolic patterns significantly distinct from the basic nutrient model.

  17. The genome of Eucalyptus grandis

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    Myburg, Alexander A.; Grattapaglia, Dario; Tuskan, Gerald A.; Hellsten, Uffe; Hayes, Richard D.; Grimwood, Jane; Jenkins, Jerry; Lindquist, Erika; Tice, Hope; Bauer, Diane; Goodstein, David M.; Dubchak, Inna; Poliakov, Alexandre; Mizrachi, Eshchar; Kullan, Anand R. K.; Hussey, Steven G.; Pinard, Desre; van der Merwe, Karen; Singh, Pooja; van Jaarsveld, Ida; Silva-Junior, Orzenil B.; Togawa, Roberto C.; Pappas, Marilia R.; Faria, Danielle A.; Sansaloni, Carolina P.; Petroli, Cesar D.; Yang, Xiaohan; Ranjan, Priya; Tschaplinski, Timothy J.; Ye, Chu-Yu; Li, Ting; Sterck, Lieven; Vanneste, Kevin; Murat, Florent; Soler, Marçal; Clemente, Hélène San; Saidi, Naijib; Cassan-Wang, Hua; Dunand, Christophe; Hefer, Charles A.; Bornberg-Bauer, Erich; Kersting, Anna R.; Vining, Kelly; Amarasinghe, Vindhya; Ranik, Martin; Naithani, Sushma; Elser, Justin; Boyd, Alexander E.; Liston, Aaron; Spatafora, Joseph W.; Dharmwardhana, Palitha; Raja, Rajani; Sullivan, Christopher; Romanel, Elisson; Alves-Ferreira, Marcio; Külheim, Carsten; Foley, William; Carocha, Victor; Paiva, Jorge; Kudrna, David; Brommonschenkel, Sergio H.; Pasquali, Giancarlo; Byrne, Margaret; Rigault, Philippe; Tibbits, Josquin; Spokevicius, Antanas; Jones, Rebecca C.; Steane, Dorothy A.; Vaillancourt, René E.; Potts, Brad M.; Joubert, Fourie; Barry, Kerrie; Pappas, Georgios J.; Strauss, Steven H.; Jaiswal, Pankaj; Grima-Pettenati, Jacqueline; Salse, Jérôme; Van de Peer, Yves; Rokhsar, Daniel S.; Schmutz, Jeremy

    2014-06-11

    Eucalypts are the world s most widely planted hardwood trees. Their broad adaptability, rich species diversity, fast growth and superior multipurpose wood, have made them a global renewable resource of fiber and energy that mitigates human pressures on natural forests. We sequenced and assembled >94% of the 640 Mbp genome of Eucalyptus grandis into its 11 chromosomes. A set of 36,376 protein coding genes were predicted revealing that 34% occur in tandem duplications, the largest proportion found thus far in any plant genome. Eucalypts also show the highest diversity of genes for plant specialized metabolism that act as chemical defence against biotic agents and provide unique pharmaceutical oils. Resequencing of a set of inbred tree genomes revealed regions of strongly conserved heterozygosity, likely hotspots of inbreeding depression. The resequenced genome of the sister species E. globulus underscored the high inter-specific genome colinearity despite substantial genome size variation in the genus. The genome of E. grandis is the first reference for the early diverging Rosid order Myrtales and is placed here basal to the Eurosids. This resource expands knowledge on the unique biology of large woody perennials and provides a powerful tool to accelerate comparative biology, breeding and biotechnology.

  18. Catalogue of Danish Alysiinae (Hymenoptera: Braconidae, with the description of two new species of Aspilota Foerster, 1863

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    Francisco Javier Peris-Felipo

    2016-12-01

    Full Text Available In the present study, a total of 153 species of Alysiinae (Hymenoptera: Braconidae from Denmark are catalogued. Two species are described as new for science: Aspilota leptoarticulata Munk & Peris-Felipo sp. nov. and A. grandis Munk & Peris-Felipo sp. nov. Additionally, 38 alysiine species are recorded for the first time for the Danish fauna. A faunistic list with distribution data and host records is provided.

  19. Starvation-induced morphological responses of the boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae)

    Science.gov (United States)

    Status of the boll weevil, Anthonomus grandis grandis Boheman, as a pest of cotton (Gossypium spp.) in the United States has diminished because of progress by eradication programs. However, this pest remains of critical importance in South America, and intractable populations in extreme South Texas ...

  20. Insectos asociados con flores de malezas del Jardín Botánico de Santiago de Cuba, con énfasis en Hymenoptera

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    José L. Fernández T.

    2001-12-01

    Full Text Available Se estudiaron los insectos visitantes en flores de nueve especies de malezas del Jardín Botánico de Santiago de Cuba, Cuba Oriental, durante 1993 (marzo a junio, en primavera y 1994 (enero a marzo, final del invierno y comienzo de la primavera. Se contabilizaron unas 50 horas de recolecta en tres horarios diferentes (0900-0930 hr en 1993; 0900-0930 hr, 1200-1230 hr y 1500-1530 hr en 1994. Se identificaron más de 140 especies y por lo menos 37 familias; Hymenoptera fue el orden mejor representado (con más de la mitad de los individuos rerecolectados, seguido de Diptera, Coleoptera y Lepidoptera. Dentro de Hymenoptera las abejas (Apoidea fueron mayoritarias, especialmente Apis mellifera L.; seguidas de avispas (Vespidae, Pompilidae, Sphecidae e icneumónidos (Ichneumonidae; los microhimenópteros no se recolectaron. Se comparó la himenopterofauna en cuanto a diversidad, similitud, especies dominantes y subdominantes, preferencia de horarios, eficiencia de los muestreos, etc. Cada especie de planta sustentó una fauna característica. Casi un tercio de los himenópteros visitantes fueron controles biológicos de plagas agrícolas, y la mayoría pueden considerarse polinizadores. Especies afines filogenéticamente presentaron patrones de actividad diaria semejante. El horario 0900 -0930 hr fue el de mayor biodiversidad en cuanto a número de especies visitantes. Al analizar el número de especies con relación al número de especímenes rerecolectados, así como el total de especies y número de muestreos realizados, las funciones de mejor ajuste fueron polinomiales de segundo grado (r² = 0.9734 y r² = 0.9573 respectivamente, p The insect visitors of flowers in nine weeds species were studied in the Botanical Garden of Santiago de Cuba, Eastern Cuba, during 1993 (March - June, spring season and 1994 (January - March, end of winter and beginning of spring season. About 50 hours of collecting efforts were made at three times (0900-0930 hr in

  1. Evolutionary dynamics of the karyotype of the wasp Trypoxylon (Trypargilum nitidum (Hymenoptera, Sphecidae from the Rio Doce State Park, Minas Gerais, Brazil

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    Ricardo Scher

    2003-01-01

    Full Text Available Cytogenetic analysis based on the distribution of C-bands showed two groups of karyotypes in a Trypoxylon nitidum population from the Rio Doce Park, State of Minas Gerais, Brazil. One of these groups, that was identical to a previously described karyotype (n = 15; 2n = 30, had a stable chromosome number and was rich in acrocentric chromosomes, whereas the other had a variable chromosome number (n = 12 to 14; 2n = 25 to 28 and was rich in pseudo-acrocentric chromosomes. We propose a hypothesis explaining the dynamics of the modifications which occurred in the karyotype of this species, based on the minimum interaction theory of Imai et al. (1986, 1988, 1994 and on the chromosome rearrangements and heteromorphisms observed by us.

  2. The origins of species richness in the Hymenoptera: insights from a family-level supertree

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    Davis Robert B

    2010-04-01

    Full Text Available Abstract Background The order Hymenoptera (bees, ants, wasps, sawflies contains about eight percent of all described species, but no analytical studies have addressed the origins of this richness at family-level or above. To investigate which major subtaxa experienced significant shifts in diversification, we assembled a family-level phylogeny of the Hymenoptera using supertree methods. We used sister-group species-richness comparisons to infer the phylogenetic position of shifts in diversification. Results The supertrees most supported by the underlying input trees are produced using matrix representation with compatibility (MRC (from an all-in and a compartmentalised analysis. Whilst relationships at the tips of the tree tend to be well supported, those along the backbone of the tree (e.g. between Parasitica superfamilies are generally not. Ten significant shifts in diversification (six positive and four negative are found common to both MRC supertrees. The Apocrita (wasps, ants, bees experienced a positive shift at their origin accounting for approximately 4,000 species. Within Apocrita other positive shifts include the Vespoidea (vespoid wasps/ants containing 24,000 spp., Anthophila + Sphecidae (bees/thread-waisted wasps; 22,000 spp., Bethylidae + Chrysididae (bethylid/cuckoo wasps; 5,200 spp., Dryinidae (dryinid wasps; 1,100 spp., and Proctotrupidae (proctotrupid wasps; 310 spp.. Four relatively species-poor families (Stenotritidae, Anaxyelidae, Blasticotomidae, Xyelidae have undergone negative shifts. There are some two-way shifts in diversification where sister taxa have undergone shifts in opposite directions. Conclusions Our results suggest that numerous phylogenetically distinctive radiations contribute to the richness of large clades. They also suggest that evolutionary events restricting the subsequent richness of large clades are common. Problematic phylogenetic issues in the Hymenoptera are identified, relating especially to

  3. Strade, ferrovie e grandi rischi

    Directory of Open Access Journals (Sweden)

    Scira Menoni

    2009-07-01

    Full Text Available Nell’articolo il rapporto tra sistemi di accessibilità (ferroviaria e stradale e grandi rischi viene affrontato da due diverse prospettive. La prima vede tali sistemi come esposti e vulnerabili a diverse forme di pericolosità naturale; la seconda li considera invece quali potenziali fonti di pericolo, indagando in particolare il trasporto di sostanze pericolose. Per quanto riguarda la prima parte dell’articolo, ovvero l’analisi di vulnerabilità delle infrastrutture di trasporto a diversi agenti naturali, verranno esplicitati i fattori di vulnerabilità fisica, sistemica e organizzativa. A sostegno della modellazione proposta, si fa riferimento sia alla letteratura internazionale sia al metodo sviluppato presso il Politecnico di Milano, nei suoi ultimi sviluppi. Interessa infatti presentare un metodo relativamente semplice da utilizzare a scala vasta per poi scendere nel dettaglio necessario laddove le priorità individuata a grande scala ne indichino la necessità. Tra i fattori di vulnerabilità fisica si indicheranno gli elementi maggiormente critici per diversi tipi di sollecitazione (sismica, vulcanica, alluvionale, etc.; per quanto attiene ai fattori di vulnerabilità sistemica si considerano le interdipendenze tra reti e sistemi (inclusi gli altri servizi a rete; per quanto attiene alla vulnerabilità organizzativa si indicheranno le difficoltà nel produrre un sistema integrato di allertamento, che consenta ad esempio di chiudere tratti di strada quando questi vengano segnalati come imminentemente interessati da un dato evento estremo. Infine si indicheranno i danni che il sistema di accessibilità può subire e le ripercussioni di questi sul territorio di interesse. Nella seconda parte viene trattato un particolare tipo di trasporto, quello delle sostanze pericolose, differenziando opportunamente tra ferroviario e stradale. Il ribaltamento del punto di vista comporta la necessità di identificare in modo puntuale i fattori di

  4. The Sphecidae (Hym

    African Journals Online (AJOL)

    BioMAP

    the most prolific of the workers in this field (Weygoldt & Paulus 1979b, Van der Hammen. 1977b, 1979, 1969 ... the Arabian Peninsula were helpful and useful guides in our work. Medically ..... Stanford University Press, Stanford, California.

  5. Micropropagation of Eucalyptus grandis and nitens using tissue culture techniques

    Energy Technology Data Exchange (ETDEWEB)

    Furze, M.J.; Creswell, C.F.

    1985-01-01

    Experiments with nodal explants of E. grandis and E. nitens seedlings and E. grandis coppice shoots showed that a large number of plants can be produced from a single explant using micropropagation. The percentage of micropropagated shoots that formed roots was about 90% for E. grandis and 80% for E. nitens. For both species, about 90% of the rooted shoots survived after hardening off. 9 references.

  6. Regeneration and transformation of Eucalyptus grandis

    OpenAIRE

    Esteki, Leila

    2013-01-01

    Dissertação de mestrado em Biologia Molecular, Biotecnologia e Bioempreendedorismo em Plantas Eucalyptus grandis is the most widely used species in planted forests in tropical and subtropical areas. The traits of interest underlying Eucalyptus breeding programs concern productivity and wood quality for the pulp and paper industry, as well as biotic and abiotic stress tolerance. The development of an efficient transformation protocol is necessary to explore eucalypt resources th...

  7. Sex allocation and sex-dependent selection for body size in Trypoxylon rogenhoferi Kohl (Hymenoptera, Sphecidae Alocação sexual e seleção sexo-dependente para tamanho de corpo em Trypoxylon rogenhoferi Kohl (Hymenoptera, Sphecidae

    Directory of Open Access Journals (Sweden)

    Rui Carlos Peruquetti

    2003-12-01

    Full Text Available Two populations of the wasp Trypoxylon rogenhoferi Kohl, 1884 from São Carlos and Luís Antônio, State of São Paulo, Brazil, were observed and sampled from May 1999 to February 2001 using trap-nests. This mass-provisioning wasp was used to test some aspects of optimal sex allocation theory. Both populations fit all the predictions of the models of Green and Brockmann and Grafen. Maternal provisions determined the size of each offspring, and females allocated well-stocked brood cells to daughters, the sex that benefits most being large. This strategy resulted in a difference in size between the sexes. In São Carlos, female weight at emergence was 1.18 times that of males, in Luís Antônio this value was 1.13. The brood cell volume was correlated with both wing length and weight at emergence in both sexes, and the chance that a given brood cell contained a male offspring decreased with increased brood cell volume. In T. rogenhoferi female body size was related to fitness. Larger females were able to collect more mass of spiders per day, the spiders they captured were heavier, and they provisioned more brood cells per day. They also produced larger daughters. For males, no relationship between body size and fitness was found, but the data were scarce. Since the patterns of provisioning were variable among different females in both study sites, it is possible that the females not follow a unique strategy for sex allocation. The sex ratio and/or investment ratio in the São Carlos population was female-biased and in Luís Antônio, male-biased. In spite of the influence of trap-nests diameters on male production in Luís Antônio, there is some evidence that in São Carlos population the local availability of prey and/or lower rate of parasitism may be major forces in determining the observed sex ratio, but further studies are necessary to verify such hypothesis.

  8. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) in Brazil.

    Science.gov (United States)

    Marsaro Júnior, A L; Peronti, A L B G; Costa, V A; Morais, E G F; Pereira, P R V S

    2016-02-01

    Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam.) de Wit (Fabaceae), Morus nigra L. (Moraceae), Citrus reticulata Blanco (Rutaceae), Tectona grandis L. f. (Verbenaceae), Anacardium occidentale (Anacardiaceae), Annona squamosa L. and Xylopia aromatica (Lam.) Mart. (Annonaceae), in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  9. Growth and yield models for Eucalyptus grandis grown in Swaziland ...

    African Journals Online (AJOL)

    The aim of this study was to develop a stand-level growth and yield model for short-rotationEucalyptus grandis grown for pulp wood production at Piggs Peak in Swaziland. The data were derived from a Nelder 1a spacing trial established with E. grandis clonal cuttings in 1998 and terminated in 2005. Planting density ...

  10. Clonal differences in log end splitting in Eucalyptus grandis in ...

    African Journals Online (AJOL)

    This paper discusses the juvenile–mature correlation of log end splitting among Eucalyptus grandis clones from two trials and how differences in splitting relate to differences in wood density, pith-to-bark gradient and growth rate. Two approximately 20-year-old Eucalyptus grandis clonal trials at Bergvliet plantation were ...

  11. Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

    Directory of Open Access Journals (Sweden)

    Onice Teresinha Dall'Oglio

    Full Text Available The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order.

  12. Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

    Science.gov (United States)

    Dall’Oglio, Onice Teresinha; Ribeiro, Rafael Coelho; Ramalho, Francisco de Souza; Fernandes, Flávio Lemes; Wilcken, Carlos Frederico; de Assis Júnior, Sebastião Lourenço; Rueda, Rosa Angélica Plata; Serrão, José Eduardo; Zanuncio, José Cola

    2016-01-01

    The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry) can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation) ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order. PMID:26954578

  13. Development during indirect organogenesis in Eucalyptus grandis x ...

    African Journals Online (AJOL)

    BQWUPC1

    isolation or from a region of the stem immediately above the callus. Roots developed ... In this study, the same clone (Eucalyptus grandis urophylla hybrid) was used and .... strands and they linked these with shoot formation, whilst. Arvore et al.

  14. Zeuxine grandis Seidenf. (Orchidaceae - an Addition to the Orchid Flora of India

    Directory of Open Access Journals (Sweden)

    Avishek Bhattacharjee

    2010-09-01

    Full Text Available Zeuxine grandis Seidenf., is being reported for the first time from India. We provide line drawing and color photo of specimen of Z. grandis in support of our treatment and to facilitate identification of the species.

  15. Wood Permeability in Eucalyptus grandis and Eucalyptus dunnii

    Directory of Open Access Journals (Sweden)

    Raphael Nogueira Rezende

    2017-12-01

    Full Text Available ABSTRACT The objective of this study was to evaluate the flow of air and water in Eucalyptus grandis and Eucalyptus dunnii wood. Wood was collected from four trees aged 37 years in an experimental plantation of the Federal University of Lavras, Brazil. Planks were cut off the basal logs to produce specimens for air and water permeability testing. Results indicated that the longitudinal permeability to air and water of E. grandis wood were, on average, 5% and 10% higher, respectively, than that of E. dunnii wood. E. grandis and E. dunnii wood showed neither air nor water flow in the test for permeability transversal to the fibers, and longitudinal permeability to air exceeded that to water by approximately 50 fold in both species.

  16. Seed viability constants for Eucalyptus grandis Constantes de viabilidade para sementes de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Jussara Bertho Fantinatti

    2007-01-01

    Full Text Available This work aimed to analyse Eucalyptus grandis W.Hill ex Maiden seed behaviour, under controlled deterioration, and to estimate viability equation constants for the species. Seeds were harvested in the growing season of 1999, and the moisture contents were adjusted from 11.3% to a range between 1.2 and 18.1% at 25ºC. The subsamples were sealed into laminate aluminium-foil packets, for storage in incubators at 40, 50 and 65±0.5ºC. The seeds presented orthodox performance, in which the constants for predicting seed longevity of E. grandis were K E = 9.661, C W = 6.467, C H = 0.03498 and C Q = 0.0002330. The usual and inverse relationship between water content and seed longevity was also observed. The lowest moisture content limit for application of the viability equation at 65ºC was 4.9%, estimated under hygroscopic equilibrium with 23% of relative humidity in the storage environment.Este trabalho teve como objetivos verificar o desempenho de sementes de Eucalyptus grandis W.Hill ex Maiden, após a deterioração em condições controladas, e obter as constantes da equação de viabilidade. As sementes foram colhidas na safra de 1999, e a umidade foi ajustada de 11,3% para valores entre 1,2 e 18,1% a 25ºC As subamostras foram acondicionadas em embalagens de alumínio termossoldadas, armazenadas a 40, 50 e 65±0,5ºC. As sementes apresentaram um desempenho ortodoxo em relação ao armazenamento. As constantes para a predição da longevidade foram K E = 9,661, C W = 6,467, C H = 0,03498 e C Q = 0,0002330. Foi observada a relação inversa entre teor de água e longevidade. O limite inferior de grau de umidade, calculado para aplicação da equação a 65ºC, foi de 4,9%, estimativa obtida sob equilíbrio higroscópico com umidade relativa de 23% no ambiente de armazenamento.

  17. Diagnosis of Hymenoptera venom allergy

    NARCIS (Netherlands)

    Bilo, BM; Rueff, F; Mosbech, H; Bonifazi, F; Oude Elberink, JNG

    2005-01-01

    The purpose of diagnostic procedure is to classify a sting reaction by history, identify the underlying pathogenetic mechanism, and identify the offending insect. Diagnosis of Hymenoptera venom allergy thus forms the basis for the treatment. In the central and northern Europe vespid (mainly Vespula

  18. Sex determination in the Hymenoptera

    NARCIS (Netherlands)

    Heimpel, George E.; de Boer, Jetske G.

    2008-01-01

    The dominant and ancestral mode of sex determination in the Hymenoptera is arrhenotokous parthenogenesis, in which diploid females develop from fertilized eggs and haploid males develop from unfertilized eggs. We discuss recent progress in the understanding of the genetic and cytoplasmic mechanisms

  19. Enkele bijzondere bijenwaarnemingen (Hymenoptera: Apidae)

    NARCIS (Netherlands)

    Raemakers, I.P.

    2000-01-01

    Some interesting records on Dutch bees (Hymenoptera: Apidae) The publication of the preliminary atlas of Dutch bees (Peeters et al. 1999) has stimulated many specialists in their mapping activity. The author reports several interesting new distribution records on ten bee-species in 1999. The Dutch

  20. Dependence of rate of germination of teak ( Tectona grandis ) seeds ...

    African Journals Online (AJOL)

    A study was conducted to determine suitable sources of teak (Tectona grandis) seeds and methods of treating the seeds to promote higher rate of germination, with the objective to supply large quantities of seedlings for developing commercial teak plantations in Ghana. The field work involved seed collection, seed pericarp ...

  1. Stomatal characteristics of Eucalyptus grandis clonal hybrids in ...

    African Journals Online (AJOL)

    This study describes the stomatal response occurring during water stress and subsequent recovery of three Eucalyptus grandis clonal hybrids. The aim was to investigate the degree to which stomatal conductance (gs) and stomatal density differ between the clonal hybrids across seasons and in response to water stress.

  2. Water relations of Eucalyptus nitens x Eucalyptus grandis : is there ...

    African Journals Online (AJOL)

    Water relations of Eucalyptus nitens x Eucalyptus grandis : is there interclonal variation in response to experimentally imposed water stress? ... Southern Forests: a Journal of Forest Science ... However, water stress reduced shoot hydraulic conductance and stem hydraulic conductivity with significant interclonal effects.

  3. Diagnosing foliar nutrient dynamics of Eucalyptus grandis in ...

    African Journals Online (AJOL)

    Fertilisation is one of the most cost-effective methods of increasing and maintaining the productivity of Eucalyptus grandis plantations in South Africa. This silvicultural practice can be optimised by using the foliar nutrient ratios measured in plants at maximum growth as a guideline for fertiliser application. The foliar nutrient ...

  4. Shoot and root morphogenesis from Eucalyptus grandis x urophylla ...

    African Journals Online (AJOL)

    Eucalyptus grandis x urophylla plantlets were regenerated via indirect organogenesis. Histological assessment of their development focused on identifying the calli, the differentiation of shoots from the calli and the shoot-root junction from the nascent shoots. Vascular tissue formation within the callus preceded that of ...

  5. Soil carbon estimation from eucalyptus grandis using canopy spectra

    African Journals Online (AJOL)

    Mapping soil fertility parameters, such as soil carbon (C), is fundamentally important for forest management and research related to forest growth and climate change. This study seeks to establish the link between Eucalyptus grandis canopy spectra and soil carbon using raw and continuum-removed spectra. Canopy-level ...

  6. Genetic improvement of Eucalyptus grandis using breeding seedling ...

    African Journals Online (AJOL)

    Eucalyptus grandis is commercially important in Zimbabwe and a breeding program has been in progress since 1962. A classical breeding strategy was used initially but, in 1981, the Multiple Population Breeding Strategy (MPBS) was implemented and the concept of the Breeding Seedling Orchard (BSO) became central to ...

  7. Prospects of Tectona Grandis as a Feedstock for Biodiesel

    International Nuclear Information System (INIS)

    Sarin, Amit; Singh, Meetu; Sharma, Neerja; Singh, N. P.

    2017-01-01

    The limited availability of fossil fuels has encouraged the need of replacement fuels of renewable nature. Among the renewable fuels, biodiesel produced from oil seeds and food wastes has been favored by the majority of researchers. In this study, Tectona Grandis seed oil has been investigated as a non-edible feedstock for biodiesel. The oil content of seed is 43% which makes it suitable for commercial production of biodiesel. The synthesis of biodiesel from T. Grandis oil was done with transesterification reaction giving high percentage yield of biodiesel which reached to 89%. The T. Grandis biodiesel was subjected to determine various physicochemical parameters by standard testing methods and found in agreement with the ASTM D-6751 and EN-14214 standards. The fatty-acid methyl ester composition for the biodiesel is composed of 42.71% oleic acid, 13.1% palmitic acid, and 31.51% linoleic acid. The biodiesel showed low oxidation stability which is attributed to high percentage of unsaturation. To address this issue, synthetic antioxidants were added to increase its resistance towards oxidation. By considering all the parameters, the present study reveals that T. Grandis seed oil is reliable for the production of biodiesel with encouraging probability in future.

  8. Prospects of Tectona Grandis as a Feedstock for Biodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Sarin, Amit, E-mail: amit.sarin@yahoo.com [Department of Physical Sciences, I.K. Gujral Punjab Technical University, Kapurthala (India); Singh, Meetu [Department of Applied Sciences, I.K. Gujral Punjab Technical University, Kapurthala (India); Sharma, Neerja [PG Department of Physics and Electronics, DAV College, Amritsar (India); Singh, N. P. [Department of Planning and External Development, I.K. Gujral Punjab Technical University, Kapurthala (India)

    2017-10-26

    The limited availability of fossil fuels has encouraged the need of replacement fuels of renewable nature. Among the renewable fuels, biodiesel produced from oil seeds and food wastes has been favored by the majority of researchers. In this study, Tectona Grandis seed oil has been investigated as a non-edible feedstock for biodiesel. The oil content of seed is 43% which makes it suitable for commercial production of biodiesel. The synthesis of biodiesel from T. Grandis oil was done with transesterification reaction giving high percentage yield of biodiesel which reached to 89%. The T. Grandis biodiesel was subjected to determine various physicochemical parameters by standard testing methods and found in agreement with the ASTM D-6751 and EN-14214 standards. The fatty-acid methyl ester composition for the biodiesel is composed of 42.71% oleic acid, 13.1% palmitic acid, and 31.51% linoleic acid. The biodiesel showed low oxidation stability which is attributed to high percentage of unsaturation. To address this issue, synthetic antioxidants were added to increase its resistance towards oxidation. By considering all the parameters, the present study reveals that T. Grandis seed oil is reliable for the production of biodiesel with encouraging probability in future.

  9. Biology of Anastrepha grandis (Diptera: Tephritidae) in Different Cucurbits.

    Science.gov (United States)

    Bolzan, Anderson; Nava, Dori E; Garcia, Flávio R M; Valgas, Ricardo A; Smaniotto, Giovani

    2015-06-01

    Anastrepha grandis (Macquart) (Diptera: Tephritidae) is one of the main pests of cucurbits in Brazil. Losses occur due to the damage caused to the fruits and the embargo on exports, as A. grandis is considered a quarantine pest in countries that import Brazilian cucurbits. This study aimed to evaluate the development of A. grandis in hosts of the Cucurbitaceae family. The hosts used were stem squash (Cucurbita pepo L.), squash (Cucurbita moschata Duchesne), chayote [Sechium edule (Jacq.) Swartz], mini watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai], Spanish melon (Cucumis melo L.), hybrid squash "Tetsukabuto" (C. moschata×Cucurbita maxima Duchesne), and salad cucumber (Cucumis sativus L.). We evaluated the viability and duration of egg-to-pupa period, pupal weight, sex ratio, and average number of pupae per fruit under controlled conditions of temperature, relative humidity, and photophase. The preoviposition and oviposition periods, fecundity, fertility, and longevity of females were determined for adults. Hosts of the genus Cucurbita provided a better development of A. grandis in comparison with other hosts, and presented a greater number of insects on fruit as well as higher infestation rate. Fecundity and longevity were also higher for females that developed in hosts of the genus Cucurbita, although values of these biological parameters varied between stem squash, squash, hybrid squash "Tetsukabuto." © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  10. Assessment of wood density of seven clones of Eucalyptus grandis ...

    African Journals Online (AJOL)

    With the objective of evaluating the correlation of wood basic density with age in seven Eucalyptus grandis clones planted in Brazil, five trees in each clone were sampled at the ages of 0, 5, 1, 5, 2, 5, 3, 5, 4, 5 and 7, 5 years. The analysis of these samples showed that the intraclonal variation of the basic density (except for 0, ...

  11. The potential of young, green finger-jointed Eucalyptus grandis ...

    African Journals Online (AJOL)

    Drying will occur naturally while the lumber is fixed within the roof truss structure. The objectives of this study were (1) to investigate the strength and stiffness variation of the finger-jointed E. grandis product in both the green and dry state for different age and dimension lumber, (2) to investigate the variation in density, warp ...

  12. Cafe Grandis kõlab lautomuusika / Anneli Remme

    Index Scriptorium Estoniae

    Remme, Anneli, 1968-

    2008-01-01

    Corelli Musicu salongiõhtutel festivali "Fiesta de la Guitarra" raames esineb trio koosseisus Stewart McCoy (lautomängija), Robert Staak (lautomängija) ja Maria Staak (laulja), esinejatest. Esitatakse renessansiaja autorite lautoduette ja -laule. Kontsert 8. nov. Pärnu hotellis sarjas "Café Grandi muusikasalong"

  13. Antibacterial, antioxidant and cell proliferative properties of Coccinia grandis fruits

    Directory of Open Access Journals (Sweden)

    Prashant Sakharkar

    2017-06-01

    Full Text Available Objective: Little knowledge is available on the antimicrobial and antioxidant properties of Coccina grandis fruits and no study has reported on its cell proliferative property. The aim of this study was to examine the antimicrobial, antioxidant and cell proliferative property of fruits of C. grandis. Material and Methods: Fruits of C. grandis were extracted using water; ethanol and acetone by cold and hot Soxhlet extraction. The antibacterial activities of the extracts were tested against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa using the modified Kirby-Bauer diffusion method and compared against erythromycin. The antioxidant property was determined using Cayman's antioxidant assay; whereas cell proliferation/cytotoxic properties were evaluated using the Cell Titer 96 Aqueous One Solution Cell MTS assay with MDA-MB 321 breast cancer cells. Data were analyzed for correlation and differences using unpaired student's t-test and one-way ANOVA. A p value of Results: Both cold and hot ethanol and acetone extracts of C. grandis fruits showed some degree of bacterial growth inhibition. Acetone extracts exhibited higher antibacterial activity. Both ethanol extracts showed antioxidant property when compared with standard Trolox. In contrary to cytotoxicity, all four extracts showed cell proliferation compared to controls at different concentrations. However, acetone extracts exhibited greater cell proliferation compared to ethanol extracts and cold extracts performed better than the hot extracts. Conclusion: C. grandis fruits exhibited some degree of antimicrobial, antioxidant and cell proliferative properties. Further investigation is warranted to isolate, confirm and characterize phytochemicals that are responsible for the medicinal properties observed.

  14. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae in Brazil

    Directory of Open Access Journals (Sweden)

    A. L. Marsaro Júnior

    Full Text Available Abstract Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam. de Wit (Fabaceae, Morus nigra L. (Moraceae, Citrus reticulata Blanco (Rutaceae, Tectona grandis L. f. (Verbenaceae, Anacardium occidentale (Anacardiaceae, Annona squamosa L. and Xylopia aromatica (Lam. Mart. (Annonaceae, in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  15. Transcriptomic survey of the midgut of Anthonomus grandis (Coleoptera: Curculionidae).

    Science.gov (United States)

    Salvador, Ricardo; Príncipi, Darío; Berretta, Marcelo; Fernández, Paula; Paniego, Norma; Sciocco-Cap, Alicia; Hopp, Esteban

    2014-01-01

    Anthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness of its control using conventional insecticide applications and biocontrol techniques. In spite of its importance, little is known about its genome sequence and, more important, its specific expression in key organs like the midgut. Total mRNA isolated from larval midguts was used for pyrosequencing. Sequence reads were assembled and annotated to generate a unigene data set. In total, 400,000 reads from A. grandis midgut with an average length of 237 bp were assembled and combined into 20,915 contigs. The assembled reads fell into 6,621 genes models. BlastX search using the NCBI-NR database showed that 3,006 unigenes had significant matches to known sequences. Gene Ontology (GO) mapping analysis evidenced that A. grandis is able to transcripts coding for proteins involved in catalytic processing of macromolecules that allows its adaptation to very different feeding source scenarios. Furthermore, transcripts encoding for proteins involved in detoxification mechanisms such as p450 genes, glutathione-S-transferase, and carboxylesterases are also expressed. This is the first report of a transcriptomic study in A. grandis and the largest set of sequence data reported for this species. These data are valuable resources to expand the knowledge of this insect group and could be used in the design of new control strategies based in molecular information. © The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.

  16. Inventario de Hymenoptera (Hexapoda en El Ventorrillo: un rico enclave de biodiversidad en la Sierra de Guadarrama (España Central

    Directory of Open Access Journals (Sweden)

    Nieves-Aldrey, José Luis

    2003-12-01

    Full Text Available Overall data of the inventory of Hymenoptera from the Biogeological Station of “El Ventorrillo” are presented. The studied site is located at an altitude of 1450 m, on the south face of the Sierra de Guadarrama (Central Spain, about 60 km NW from Madrid. Between 1988 and 1991 an insect biodiversity inventory was carried out using three sampling methods: Malaise traps, yellow pan traps and sweep nets. Out of the more than 1,000,000 insects trapped, increasing the collections of the MNCN, about 600,000 were sorted to order. We found 83,688 individuals of Hymenoptera (representing 13,8% and the second more abundant group in the samples, after Diptera (450,000 individuals and 77,5% of total. Forty nine families, 518 genera and 1310 species de Hymenoptera has been identified until now. The overall richness of Hymenoptera from El Ventorrillo is estimated in 2700 species and about 13,000 the number of insect species from the study site. An appendix is provided with the list of identified species and its overall abundance in the samples. As results of the inventory, ten new species for science have been described, and several more new species are not yet described; additionally, at least 33 genera and more than 170 species were recorded for the first time for Iberia. The abundance of Hymenoptera, as measured by Malaise trap catches, was very high, comparatively to other published data, reaching a peak of 916 individuals per trap day at the most productive trap and sampling period. The more abundant families were, in decreasing order, Braconidae, Ichneumonidae, Mymaridae, Scelionidae, Apidae and Pteromalidae, represented by numbers of individuals between 12,000, for Braconidae, to near 6000 for Pteromalidae. Among the identified families, the more species rich at the study area were in decreasing order: Pteromalidae (290, Ichneumonidae (217, Sphecidae (107 and Eulophidae (101 species. The richness of the 29 remaining families at the area of study was

  17. Actividad antianémica de la Cassia grandis L. The antianemic activity of Cassia grandis L.

    Directory of Open Access Journals (Sweden)

    Juana Tillán Capó

    2004-12-01

    Full Text Available La población de la zona oriental de Cuba refiere los efectos beneficiosos del uso tradicional de la Cassia grandis L. en la anemia, con la utilización del polvo seco obtenido del fruto como un suplemento nutricional. El objetivo fue evaluar dicho efecto en un modelo experimental de anemia ferropénica en ratas, inducido por sucesivas extracciones de sangre y administración de una dieta carente de hierro. Todos los animales fueron sometidos durante 15 días a una dieta semisintética deficiente en hierro y a extracciones de sangre 3 veces por semana hasta lograr concentraciones de hemoglobina en sangre menores de 9 g/dL. Se formaron 3 grupos y se mantuvo la misma dieta: grupo I sin suplementar, grupo II suplementado con 15 mg de hierro/kg de dieta y grupo III la misma cantidad de hierro más 750 mg/kg de peso corporal de polvo seco de Cassia grandis L. durante otros 15 días. Al finalizar se determinaron las concentraciones de hierro, hemoglobina y hematócrito en sangre. Las concentraciones medias de hemoglobina al cabo de los 15 días de tratamiento fueron significativamente diferentes en los 3 grupos experimentales, con resultados mayores en el grupo suplementado con hierro y Cassia grandis L.; también en este grupo se observó un incremento significativo de los valores medios de hierro en plasma en relación con los valores obtenidos en los animales no suplementados y en los animales que recibieron solamente hierro en la dieta. El porcentaje de hematócrito no mostró diferencia significativa entre tratamiento. Los resultados corroboran el uso popular y tradicional de la Cassia grandis L. en los estados anémicos, al mejorar la utilización del hiero y la producción de hemoglobina.The population of the eastern zone of Cuba refers to the benefitial effects of the traditional use of Cassia grandis L. in anemia by using dry powder obtained from the fruit as a nutritional supplement. The objective was to evaluate this effect in an

  18. Boll weevil (Anthonomus grandis) population genomics as a tool for monitoring and management

    Science.gov (United States)

    Despite the success of eradication efforts across most of the cotton-producing regions of the U.S., the cotton boll weevil (Anthonomus grandis grandis Boheman) remains a major pest of cotton in much of the New World. The area along the Texas border with northern Mexico has been a particularly troub...

  19. Long-Term Effects of Exotic Tree Species ( Tectona grandis Linn. F ...

    African Journals Online (AJOL)

    Long-Term Effects of Exotic Tree Species ( Tectona grandis Linn. F.) on the Status of Extractable Micronutrients in the ... The study therefore implied that Tectona grandis has an extractive property on micronutrient particularly on soils that are low in these nutrients. Nigerian Journal of Soil and Environmental Research Vol.

  20. Grandi Eventi: indicatori di classificazione e incidenza sui sistemi urbani

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    Giuseppe Mazzeo

    2008-08-01

    Full Text Available I grandi eventi sono ritenuti una opportunità per le città in quanto sono uno straordinario catalizzatore di investimenti mirati alla trasformazione della città. L’azione dei grandi eventi non è confinata solo nel periodo di svolgimento dell’evento stesso ma si estende ad un periodo molto più ampio, prima e, soprattutto, dopo il suo termine. Nell’organizzazione delle manifestazioni più recenti le trasformazioni urbane hanno interessato in modo sempre maggiore parti già in precedenza urbanizzate, al punto che l’evento diviene l’occasione per trasformare la città costruita e per modificarne il profilo. Il paper approfondisce l’argomento della classificazione degli eventi e del loro impatto sul sistema urbano sviluppandosi in tre parti. Nella prima si analizzano due tipologie di grandi eventi (le esposizioni internazionali e i giochi olimpici con l’obiettivo di pervenire ad una definizione condivisa. Nella seconda parte si identificano i più importanti fattori ed indicatori per l’analisi di questa specifica categoria di avvenimenti. Infine, nella terza parte, si approfondiscono i fattori connessi all’impatto sul sistema urbano e all’organizzazione della sua mobilità. In particolare, viene approfondito il concetto di “effetto pulsar”, ossia gli effetti moltiplicativi sulla evoluzione urbana dipendenti dalla organizzazione di eventi multipli in parallelo o in sequenza. Uno degli elementi più negativi connessi alla organizzazione di un grande evento è la dispersione nel tempo dei benefici acquisiti grazie ad esso; per evitare ciò è necessario che l’evento sia seguito da politiche strategiche continue in modo da preservare i vantaggi acquisiti.

  1. ECONOMIC ROTATION OF Eucalyptus grandis PLANTATIONS FOR PULP PRODUCTION

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    Thais Cunha Ferreira

    2004-07-01

    Full Text Available The objectives of the research were: to determine the economic impact of several minimum diameter and length of logs in economic rotation age, economic feasibility of Eucalyptus grandis plantation for cellulose production; to determine the economic loss of cutting the stand before or after the optimal economic rotation age. A biometric model for making wood volume prognosis was developed using data of a trial of Eucalyptus grandis plantation envisaging pulp production. Eucalyptus grandis stands of 19 and 103 months old, in the spacing 3 x 2 and 3 x 3 m in site index of 30; 28; 26 and 24 m were used. Theprognosis started at the age zero, considering logs of 2.5; 2.8; 4.0 and 6.0 m of length for minimum diameter varying from 4 to 10 cm, in intervals of 2 cm. Net Present Worth (VPL was used the economic criterion, considering an infinite horizon and a cost relation including reestablishment, yearly maintenance, logging and wood transportation costs. The main conclusions were: increases in the minimum diameter and or in logs length increase the rotation age; harvesting the stands in ages different from the optimal one cause large economic loss mainly in the better sites; the economic loss is larger if the harvest is made before the optimal economic rotation than if it is make after; economic feasibility increases when the minimum diameter is smaller and when the length of the logs is shorter. Any way, before making any decision it is necessary to take into account possible technical restrictions and effect on harvest and transportation costs caused by changer in the length of logs and in the size of the minimum commercial diameter.

  2. Hymenoptera stings in Brazil: a neglected health threat in Amazonas State

    OpenAIRE

    Costa, Allyson Guimarães; Chaves, Bárbara Aparecida; Murta, Felipe Leão Gomes; Sachett, Jacqueline Almeida Gonçalves; Sampaio, Vanderson Souza; Silva, Vanessa Costa; Monteiro, Wuelton Marcelo

    2018-01-01

    Abstract INTRODUCTION: Hymenoptera injuries are commonly caused by stinging insects. In Amazonas state, Brazil, there is no information regarding distribution, profile, and systemic manifestations associated with Hymenoptera injuries. METHODS: This study aimed to identify risk factors for systemic manifestation using the Brazilian Notifiable Diseases Surveillance System (2007 to 2015). RESULTS: Half of Hymenoptera injuries were caused by bee stings. Hymenoptera injuries were concentrate...

  3. Sanitary and nutritional characterization of honeybee colonies in Eucalyptus grandis plantations

    OpenAIRE

    Invernizzi, C.; Santos, E.; García, E.; Daners, G.; Di Landro, R.; Saadoun, A.; Cabrera, C.

    2011-01-01

    In Uruguay, many beekeepers transport their colonies to Eucalyptus grandis plantations at the end of the summer and autumn, obtaining important honey harvests. However, at the end of the flowering period the colonies become extremely weakened undergoing high levels of mortality. Nutritional and health problems could explain the weakening of colonies. In order to find out the causes for this weakening, colonies of the same size were taken to an E. grandis plantation, split up in three groups d...

  4. Karyotypic analysis of the cotton boll weevil, Anthonomus grandis Boheman.

    Science.gov (United States)

    McNally, L R; Beck, M L; Biggers, C J

    2000-01-01

    The diploid chromosome number of the cotton boll weevil, Anthonomus grandis Boheman, is 44. Both C- and N-banding techniques of mitotic cells demonstrated constitutive heterochromatin in the p arm of the eight largest chromosomes, the p arm of the X chromosome, and the centromeric region of autosomal groups A-D. Neither the y nor the group E autosomes appeared to contain constitutive heterochromatin. Supernumerary chromosomes were not found in the boll weevil. Restriction endonuclease banding of primary spermatocytes revealed a rod-shaped Xy tetrad in which the X and y were terminally associated. The p arm of the large, submetacentric X was C-band positive. While two of the autosomal tetrads were typically ring-shaped in primary spermatocytes, the remaining 19 autosomal tetrads were rod-shaped.

  5. [Habitat factor analysis for Torreya grandis cv. Merrillii based on spatial information technology].

    Science.gov (United States)

    Wang, Xiao-ming; Wang, Ke; Ao, Wei-jiu; Deng, Jin-song; Han, Ning; Zhu, Xiao-yun

    2008-11-01

    Torreya grandis cv. Merrillii, a tertiary survival plant, is a rare tree species of significant economic value and expands rapidly in China. Its special habitat factor analysis has the potential value to provide guide information for its planting, management, and sustainable development, because the suitable growth conditions for this tree species are special and strict. In this paper, the special habitat factors for T. grandis cv. Merrillii in its core region, i.e., in seven villages of Zhuji City, Zhejiang Province were analyzed with Principal Component Analysis (PCA) and a series of data, such as IKONOS image, Digital Elevation Model (DEM), and field survey data supported by the spatial information technology. The results showed that T. grandis cv. Merrillii exhibited high selectivity of environmental factors such as elevation, slope, and aspect. 96.22% of T. grandis cv. Merrillii trees were located at the elevation from 300 to 600 m, 97.52% of them were found to present on the areas whose slope was less than 300, and 74.43% of them distributed on sunny and half-sunny slopes. The results of PCA analysis indicated that the main environmental factors affecting the habitat of T. grandis cv. Merrillii were moisture, heat, and soil nutrients, and moisture might be one of the most important ecological factors for T. grandis cv. Merrillii due to the unique biological and ecological characteristics of the tree species.

  6. Hymenoptera Stings and the Acute Kidney Injury

    Directory of Open Access Journals (Sweden)

    Yashad Dongol

    2013-07-01

    Full Text Available Hymenoptera stings are a health concern. Apidae (bees, Vespidae (hornets, yellow jackets and wasps and Formicidae (ants are medically-important stinging insects under the order Hymenoptera. Clinical features from simple skin manifestations to severe and fatal organ injury are due to the hypersensitivity reactions and/ or the toxic effects of the venom inoculated. Here we discuss on Hymenoptera stings involving apids (honey bees and vespids (wasps, hornets and yellow jackets and their effect on renal function and associated morphological changes in the kidney. Despite the differences in venom composition and quantity released per sting in two insect groups, both lead to similar medical consequences, such as localised normal allergic reactions, mild to severe anaphylaxis and shock and multiple organ and tissue injury leading to multiple organ failure. Acute kidney injury (AKI is one of the unusual complications of Hymenoptera stings and has the basis of both immune-mediated and toxic effects. Evidence has proven that supportive therapy along with the standard medication is very efficient in completely restoring the kidney function without any recurrence.

  7. HYMENOPTERA ALLERGENS: FROM VENOM TO VENOME

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    Edzard eSpillner

    2014-02-01

    Full Text Available In Western Europe hymenoptera venom allergy primarily relates to venoms of the honeybee and the common yellow jacket. In contrast to other allergen sources, only a few major components of hymenoptera venoms had been characterized until recently. Improved expression systems and proteomic detection strategies have allowed the identification and characterization of a wide range of additional allergens. The field of hymenoptera venom allergy research has moved rapidly from focusing on venom extract and single major allergens to a molecular understanding of the entire venome as a system of unique and characteristic components. An increasing number of such components has been identified, characterized regarding function and assessed for allergenic potential. Moreover, advanced expression strategies for recombinant production of venom allergens allow selective modification of molecules and provide insight into different types of IgE reactivities and sensitization patterns. The obtained information contributes to an increased diagnostic precision in hymenoptera venom allergy and may serve for monitoring, reevaluation and improvement of current therapeutic strategies.

  8. De wespbijen (Nomada) van Nederland (Hymenoptera: Apidae)

    NARCIS (Netherlands)

    Smit, J.

    2004-01-01

    The wasp bees (Nomada) of the Netherlands (Hymenoptera: Apidae) In the Netherlands 44 species of Nomada are known to occur. The name wasp bee is derived from the often yellow and black colour, which give the insects a wasplike appearance. Nomada bees are cleptoparasites mostly with Andrena species.

  9. Basophil-activation tests in hymenoptera allergy

    NARCIS (Netherlands)

    Dubois, Anthony E. J.; van der Heide, Sicco

    The measurement of basophil-activation markers may be useful in detecting IgIE-mediated sensitization but the relevance for application of the basophil-activation test in prediction of clinical reactivity in Hymenoptera allergy is very limited. For this reason, this test currently has no established

  10. Estimativa do tempo de vaporização de toras de Eucalyptus grandis Steaming times estimates for Eucalyptus grandis logs

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    Fred Willians Calonego

    2006-06-01

    Full Text Available O objetivo deste estudo foi adequar o modelo geral de determinação do tempo de vaporização de toras, proposto por Steinhagen et al. (1980, para a madeira de Eucalyptus grandis. Para tanto, foram coletadas toras de 20 a The aim this study was to adjust the general model for determining log steaming time, proposed by Steinhagen et al. (1980, for Eucalyptus grandis wood. In order to do so, logs with diameter from 20 to <25, 25 to <30 and 30 to <35 cm were collected from 14 trees of Eucalyptus grandis derived from the `Horto Florestal' nursery, Manduri, São Paulo. A thermocouple was inserted into each log near its center. The logs were steamed during 20 hours at 90ºC and 100% relative humidity. A data logger recorded the temperatures during the thermal treatment. It was concluded that the Steinhagen et al. (1980 model cannot be directly used for this species in study and corrections factors are proposed for the utilization of the general model for log steaming time, developed by Steinhagen et al. (1980, for Eucalyptus grandis wood.

  11. Single locus complementary sex determination in Hymenoptera : an "unintelligent" design?

    NARCIS (Netherlands)

    Wilgenburg, Ellen van; Driessen, Gerard; Beukeboom, Leo W.

    2006-01-01

    The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid) has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding,

  12. Single locus complementary sex determination in Hymenoptera: an "unintelligent" design?

    NARCIS (Netherlands)

    van Wilgenburg, E.; Driessen, G.J.J.; Beukeboom, L.W.

    2006-01-01

    The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid) has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding,

  13. ECONOMIC EVALUATION OF Eucalyptus grandis PLANTATION FOR CELLULOSE PRODUCTION

    Directory of Open Access Journals (Sweden)

    Antonio Donizette de Oliveira

    2008-03-01

    Full Text Available The aims of this research were: to analyze the economic feasibility of planting eucalyptus for producing wood pulp,considering various site index and two spacings; to analyze the economic effects regarding the profitability of the forest activity indifferent distances from the industry and changes on discount rate, wood price, transportation costs, minimum profitable diameter oflogs and the length of the logs. A biometric model for making wood volume prognosis was developed, using data of a trial ofEucalyptus grandis stands 19 and 103 months old. The prognosis started at the age zero, considering logs of 2.5 and 6.0 m of lenghtand the minimum diameter varying from 4 to 10 cm, in intervals of 2 cm. Net Present Worth (NPW was used as the economic decisioncriterium, considering an infinite horizon. The main conclusions were: reducing the minimum profitable diameter and the length ofthe logs are good strategies to increase wood utilization and profit; plantations located in less productive lands are economicallyunfeasible; the cost of transportation has significant effect on the profitability of the forest activity and must be analyzed carefully atthe moment of defining the location of new plantations; small variations on wood sales price may cause big alterations on theprofitability of the forest activity, suggesting that the improvement of the wood quality together with other decisions that may increasewood price are alternatives that may render the plantations in less productive areas profitable.

  14. Th, Pa and U isotopes in an echinoderm, Encope grandis

    International Nuclear Information System (INIS)

    Omura, Akio; Ku, Teh-Lung.

    1979-01-01

    The application of 230 Th and 231 Pa growth methods to the hard tissues of living things, which are effective for the radiometric age measurement for latter Quaternary period, has been limited to certain corals, therefore it has been scarcely utilized in other areas than coral reefs. Reef coral fossils (Porites) were obtained from terrace deposits of Magdalena Island in Southern Baja California, and the methods were applied to them. At the time, the isotope compositions of Th, Pa and U in the shells of echinoderm Encope Grandis and of the living samples were examined. The estimated ages were in agreement with those of coral. It suggests that the reliable 230 Th and 231 Pa ages of sea-urchin fossils were presented for the first time and that the method is applicable to such fossils only if the conditions can be met. The results are highly significant, since the method may be used in other areas than coral reefs. (J.P.N.)

  15. Morphology, diet, and temperature dependent host-free survival of the boll weevil, Anthonomus grandis (Coleoptera: Curculionidae)

    Science.gov (United States)

    The boll weevil, Anthonomus grandis grandis Boheman, is an important pest of cotton (Gossypium spp.) in South America, Mexico, and southernmost Texas in the United States. A key factor in the persistence of the boll weevil is its ability to survive the non-cotton season. Mechanisms facilitating this...

  16. Population structure and genetic diversity of the boll weevil, Anthonomus grandis (Coleoptera: Curculionidae), on Gossypium in North America

    Science.gov (United States)

    While the boll weevil, Anthonomus grandis, has been identified as one of the most devastating pests in U.S. history, its origin and activity in Mexico, both on wild and cultivated cotton hosts (genus Gossypium), is poorly understood. Three forms (geographical or host-associated races) of A. grandis ...

  17. Effect of thermal modification on the physical properties of juvenile and mature woods of Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Fred Willians Calonego

    Full Text Available This study aimed to evaluate the effect of thermal treatment on the physical properties of juvenile and mature woods of Eucalyptus grandis. Boards were taken from 30-year-old E. grandis trees. The boards were thermally modified at 180 °C in the Laboratory of Wood Drying and Preservation at UNESP, Botucatu, Sao Paulo state, Brazil. The results showed that thermal modification caused: (1 decrease of 6.8% in the density at 0% equilibrium moisture content of mature wood; (2 significant decreases of 14.7% and 35.6% in the maximum volumetric swellings of juvenile and mature woods, respectively; (3 significant decreases of 13.7% and 21.3% in the equilibrium moisture content of juvenile and mature woods, respectively. The influence of thermal modification in juvenile wood was lower than in mature wood and caused greater uniformity in the physical variations between these types of wood in E. grandis.

  18. Eucalyptus grandis AND Eucalyptus dunnii USE FOR WOOD-CEMENT PANELS MANUFACTURING

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2008-03-01

    Full Text Available This research evaluated the potential use of Eucalyptus grandis and Eucalyptus dunnii wood for wood-cement panelsmanufacturing. The boards were manufactured at the density of 1,20 g/cm³, using portland cement as mineral bonding and woodfurnish without treatment, treated in cold water and hot water. The wood furnish of Pinus taeda was used as control. The resultsindicated that it is not necessary to treat E. grandis and E. dunni wood for wood-cement board manufacturing. In relation to woodspecies, the board manufactured with E. dunnii showed lower values of mechanical properties. However, boards manufactured of E.grandis wood showed satisfactory results in comparison to boards of P. taeda and the referenced values of BISON process and otherproducts cited in the pertnent literature, indicating the high potential for wood-cement board manufacture of this tree species.

  19. Component Resolved Diagnosis in Hymenoptera Anaphylaxis.

    Science.gov (United States)

    Tomsitz, D; Brockow, K

    2017-06-01

    Hymenoptera anaphylaxis is one of the leading causes of severe allergic reactions and can be fatal. Venom-specific immunotherapy (VIT) can prevent a life-threatening reaction; however, confirmation of an allergy to a Hymenoptera venom is a prerequisite before starting such a treatment. Component resolved diagnostics (CRD) have helped to better identify the responsible allergen. Many new insect venom allergens have been identified within the last few years. Commercially available recombinant allergens offer new diagnostic tools for detecting sensitivity to insect venoms. Additional added sensitivity to nearly 95% was introduced by spiking yellow jacket venom (YJV) extract with Ves v 5. The further value of CRD for sensitivity in YJV and honey bee venom (HBV) allergy is more controversially discussed. Recombinant allergens devoid of cross-reactive carbohydrate determinants often help to identify the culprit venom in patients with double sensitivity to YJV and HBV. CRD identified a group of patients with predominant Api m 10 sensitization, which may be less well protected by VIT, as some treatment extracts are lacking this allergen. The diagnostic gap of previously undetected Hymenoptera allergy has been decreased via production of recombinant allergens. Knowledge of analogies in interspecies proteins and cross-reactive carbohydrate determinants is necessary to distinguish relevant from irrelevant sensitizations.

  20. Hymenoptera venom review focusing on Apis mellifera

    Directory of Open Access Journals (Sweden)

    P. R. de Lima

    2003-01-01

    Full Text Available Hymenoptera venoms are complex mixtures containing simple organic molecules, proteins, peptides, and other bioactive elements. Several of these components have been isolated and characterized, and their primary structures determined by biochemical techniques. These compounds are responsible for many toxic or allergic reactions in different organisms, such as local pain, inflammation, itching, irritation, and moderate or severe allergic reactions. The most extensively characterized Hymenoptera venoms are bee venoms, mainly from the Apis genus and also from social wasps and ant species. However, there is little information about other Hymenoptera groups. The Apis venom presents high molecular weight molecules - enzymes with a molecular weight higher than 10.0 kDa - and peptides. The best studied enzymes are phospholipase A2, responsible for cleaving the membrane phospholipids, hyaluronidase, which degrades the matrix component hyaluronic acid into non-viscous segments and acid phosphatase acting on organic phosphates. The main peptide compounds of bee venom are lytic peptide melittin, apamin (neurotoxic, and mastocyte degranulating peptide (MCD.

  1. Transcriptome analysis in cotton boll weevil (Anthonomus grandis and RNA interference in insect pests.

    Directory of Open Access Journals (Sweden)

    Alexandre Augusto Pereira Firmino

    Full Text Available Cotton plants are subjected to the attack of several insect pests. In Brazil, the cotton boll weevil, Anthonomus grandis, is the most important cotton pest. The use of insecticidal proteins and gene silencing by interference RNA (RNAi as techniques for insect control are promising strategies, which has been applied in the last few years. For this insect, there are not much available molecular information on databases. Using 454-pyrosequencing methodology, the transcriptome of all developmental stages of the insect pest, A. grandis, was analyzed. The A. grandis transcriptome analysis resulted in more than 500.000 reads and a data set of high quality 20,841 contigs. After sequence assembly and annotation, around 10,600 contigs had at least one BLAST hit against NCBI non-redundant protein database and 65.7% was similar to Tribolium castaneum sequences. A comparison of A. grandis, Drosophila melanogaster and Bombyx mori protein families' data showed higher similarity to dipteran than to lepidopteran sequences. Several contigs of genes encoding proteins involved in RNAi mechanism were found. PAZ Domains sequences extracted from the transcriptome showed high similarity and conservation for the most important functional and structural motifs when compared to PAZ Domains from 5 species. Two SID-like contigs were phylogenetically analyzed and grouped with T. castaneum SID-like proteins. No RdRP gene was found. A contig matching chitin synthase 1 was mined from the transcriptome. dsRNA microinjection of a chitin synthase gene to A. grandis female adults resulted in normal oviposition of unviable eggs and malformed alive larvae that were unable to develop in artificial diet. This is the first study that characterizes the transcriptome of the coleopteran, A. grandis. A new and representative transcriptome database for this insect pest is now available. All data support the state of the art of RNAi mechanism in insects.

  2. Transcriptome analysis in cotton boll weevil (Anthonomus grandis) and RNA interference in insect pests.

    Science.gov (United States)

    Firmino, Alexandre Augusto Pereira; Fonseca, Fernando Campos de Assis; de Macedo, Leonardo Lima Pepino; Coelho, Roberta Ramos; Antonino de Souza, José Dijair; Togawa, Roberto Coiti; Silva-Junior, Orzenil Bonfim; Pappas, Georgios Joannis; da Silva, Maria Cristina Mattar; Engler, Gilbert; Grossi-de-Sa, Maria Fatima

    2013-01-01

    Cotton plants are subjected to the attack of several insect pests. In Brazil, the cotton boll weevil, Anthonomus grandis, is the most important cotton pest. The use of insecticidal proteins and gene silencing by interference RNA (RNAi) as techniques for insect control are promising strategies, which has been applied in the last few years. For this insect, there are not much available molecular information on databases. Using 454-pyrosequencing methodology, the transcriptome of all developmental stages of the insect pest, A. grandis, was analyzed. The A. grandis transcriptome analysis resulted in more than 500.000 reads and a data set of high quality 20,841 contigs. After sequence assembly and annotation, around 10,600 contigs had at least one BLAST hit against NCBI non-redundant protein database and 65.7% was similar to Tribolium castaneum sequences. A comparison of A. grandis, Drosophila melanogaster and Bombyx mori protein families' data showed higher similarity to dipteran than to lepidopteran sequences. Several contigs of genes encoding proteins involved in RNAi mechanism were found. PAZ Domains sequences extracted from the transcriptome showed high similarity and conservation for the most important functional and structural motifs when compared to PAZ Domains from 5 species. Two SID-like contigs were phylogenetically analyzed and grouped with T. castaneum SID-like proteins. No RdRP gene was found. A contig matching chitin synthase 1 was mined from the transcriptome. dsRNA microinjection of a chitin synthase gene to A. grandis female adults resulted in normal oviposition of unviable eggs and malformed alive larvae that were unable to develop in artificial diet. This is the first study that characterizes the transcriptome of the coleopteran, A. grandis. A new and representative transcriptome database for this insect pest is now available. All data support the state of the art of RNAi mechanism in insects.

  3. Multiplicação in vitro de Eucalyptus grandis sob estímulo com benzilaminopurina In vitro multiplication of Eucalyptus grandis under BAP pulse

    Directory of Open Access Journals (Sweden)

    Wirifran Fernandes de Andrade

    2006-12-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos do estímulo com benzilaminopurina (BAP na multiplicação in vitro de Eucalyptus grandis. Foram avaliadas as interações entre concentrações de BAP (0, 200, 400 e 600 mg L-1, tempo de exposição (1, 2 e 3 horas, pH (3 e 5,8 da solução e alterações morfológicas dos explantes. Semanalmente, foi determinada a massa da matéria fresca dos explantes. Aos 21 dias de cultura, foram avaliados: o número de brotações por tratamento, o número de brotações obtidas por explante (taxa de multiplicação, e foi realizado o resgate das plântulas para avaliação histológica. O pH não apresentou interação com os demais fatores estudados. Os tratamentos com BAP a 200 mg L-1, durante 1 e 2 horas, apresentaram-se como os mais indicados na multiplicação do E. grandis. Houve intensificação da divisão celular, no parênquima cortical e no procâmbio, representada pelo surgimento de meristemóides, em resposta aos tratamentos com 200 mg L-1 de BAP, durante 1 e 2 horas. O estímulo com BAP na multiplicação in vitro de E. grandis é viável.The objective of this work was to evaluate the effect of pulse on in vitro multiplication of Eucalyptus grandis. Interactions were evaluated among BAP concentrations (0, 200, 400 and 600 mg L-1, exposure time (1, 2 and 3 hours, pH values (3 and 5.8, and explant morphological changes. Fresh weight of the explants was determined weekly. At 21 days of culture, valuations were made of the number of shootings per treatment, number of shootings obtained per explant (multiplication rate, and seedlings rescue was accomplished for histological analysis. The pH values did not present any interaction with the other factors. The most significant treatments on E. grandis shoot multiplications were 200 mg L-1 of 6-BAP during 1 and 2 hours. There was an intensification of cell division in the cortical parenchyma and procambium, represented by the arising of meristems in

  4. Anthraquinones and naphtoquinones from the bark of Tectona grandis (Verbenaceae) reforestation specimen

    OpenAIRE

    Moreira, Rafael Y.O.; Arruda, Mara S.P.; Arruda, Alberto C.; Santos, Lourivaldo S.; Müller, Adolfo H.; Guilhon, Giselle M.S.P.; Santos, Alberdan S.; Terezo, Evaristo

    2006-01-01

    O fracionamento do extrato hexânico do caule de um espécime de reflorestamento de Tectona grandis (Verbenaceae), através de procedimentos fitoquímicos clássicos, levou ao isolamento das naftoquinonas lapachol e desidro-a-lapachona e das antraquinonas tectoquinona e obtusifolina. As estruturas das substâncias foram caracterizadas através da análise de métodos espectrométricos de RMN. Este é o primeiro estudo fitoquímico de um espécime de reflorestamento de Tectona grandis, no Brasil, sendo o o...

  5. Diversity of peptidic and proteinaceous toxins from social Hymenoptera venoms.

    Science.gov (United States)

    Dos Santos-Pinto, José Roberto Aparecido; Perez-Riverol, Amilcar; Lasa, Alexis Musacchio; Palma, Mario Sergio

    2018-06-15

    Among venomous animals, Hymenoptera have been suggested as a rich source of natural toxins. Due to their broad ecological diversity, venom from Hymenoptera insects (bees, wasps and ants) have evolved differentially thus widening the types and biological functions of their components. To date, insect toxinology analysis have scarcely uncovered the complex composition of bee, wasp and ant venoms which include low molecular weight compounds, highly abundant peptides and proteins, including several allergens. In Hymenoptera, these complex mixtures of toxins represent a potent arsenal of biological weapons that are used for self-defense, to repel intruders and to capture prey. Consequently, Hymenoptera venom components have a broad range of pharmacological targets and have been extensively studied, as promising sources of new drugs and biopesticides. In addition, the identification and molecular characterization of Hymenoptera venom allergens have allowed for the rational design of component-resolved diagnosis of allergy, finally improving the outcome of venom immunotherapy (VIT). Until recently, a limited number of Hymenoptera venoms had been unveiled due to the technical limitations of the approaches used to date. Nevertheless, the application of novel techniques with high dynamic range has significantly increased the number of identified peptidic and proteinaceous toxins. Considering this, the present review summarizes the current knowledge about the most representative Hymenoptera venom peptides and proteins which are under study for a better understanding of the insect-caused envenoming process and the development of new drugs and biopesticides. Copyright © 2018 Elsevier Ltd. All rights reserved.

  6. Different processing of CAPA and pyrokinin precursors in the giant mealworm beetle Zophobas atratus (Tenebrionidae) and the boll weevil Anthonomus grandis grandis (Curculionidae).

    Science.gov (United States)

    Neupert, Susanne; Marciniak, Pawel; Köhler, Rene; Nachman, Ronald J; Suh, Charles P-C; Predel, Reinhard

    2018-03-01

    Capa and pyrokinin (pk) genes in hexapods share a common evolutionary origin. Using transcriptomics and peptidomics, we analyzed products of these genes in two beetles, the giant mealworm beetle (Zophobas atratus; Tenebrionidae) and the boll weevil (Anthonomus grandis grandis; Curculionidae). Our data revealed that even within Coleoptera, which represents a very well-defined group of insects, highly different evolutionary developments occurred in the neuropeptidergic system. These differences, however, primarily affect the general structure of the precursors and differential processing of mature peptides and, to a lesser degree, the sequences of the active core motifs. With the differential processing of the CAPA-precursor in Z. atratus we found a perfect example of completely different products cleaved from a single neuropeptide precursor in different cells. The CAPA precursor in abdominal ganglia of this species yields primarily periviscerokinins (PVKs) whereas processing of the same precursor in neurosecretory cells of the subesophageal ganglion results in CAPA-tryptoPK and a novel CAPA-PK. Particularly important was the detection of that CAPA-PK which has never been observed in the CNS of insects before. The three different types of CAPA peptides (CAPA-tryptoPK, CAPA-PK, PVK) each represent potential ligands which activate different receptors. In contrast to the processing of the CAPA precursor from Z. atratus, no indications of a differential processing of the CAPA precursor were found in A. g. grandis. These data suggest that rapid evolutionary changes regarding the processing of CAPA precursors were still going on when the different beetle lineages diverged. The sequence of the single known PVK of A. g. grandis occupies a special position within the known PVKs of insects and might serve asa basis to develop lineage-specific peptidomimetics capable of disrupting physiological processes regulated by PVKs. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. Description and Phylogeny of Urostyla grandis wiackowskii subsp. nov. (Ciliophora, Hypotricha) from an Estuarine Mangrove in Brazil.

    Science.gov (United States)

    Paiva, Thiago da Silva; Shao, Chen; Fernandes, Noemi Mendes; Borges, Bárbara do Nascimento; da Silva-Neto, Inácio Domingos

    2016-01-01

    Interphase specimens, aspects of physiological reorganization and divisional morphogenesis were investigated in a strain of a hypotrichous ciliate highly similar to Urostyla grandis Ehrenberg, (type species of Urostyla), collected from a mangrove area in the estuary of the Paraíba do Sul river (Rio de Janeiro, Brazil). The results revealed that albeit interphase specimens match with the known morphologic variability in U. grandis, morphogenetic processes have conspicuous differences. Parental adoral zone is entirely renewed during morphogenesis, and marginal cirri exhibit a unique combination of developmental modes, in which left marginal rows originate from multiple anlagen arising from innermost left marginal cirral row, whereas right marginal ciliature originates from individual within-row anlagen. Based on such characteristics, a new subspecies, namely U. grandis wiackowskii subsp. nov. is proposed, and consequently, U. grandis grandis Ehrenberg, stat. nov. is established. Bayesian and maximum-likelihood analyses of the 18S rDNA unambiguously placed U. grandis wiackowskii as adelphotaxon of a cluster formed by other U. grandis sequences. The implications of such findings to the systematics of Urostyla are discussed. © 2015 The Author(s) Journal of Eukaryotic Microbiology © 2015 International Society of Protistologists.

  8. [Community structure of soil fauna in Eucalyptus grandis plantations at different slope locations].

    Science.gov (United States)

    Zhao, Yu; Zhong, Yu; Zhang, Jian; Yang, Wan-qin

    2010-09-01

    To understand the effects of slope location on the community structure of soil fauna in Eucalyptus grandis plantation, an investigation was made on the soil fauna in 3 E. grandis plantations at different slope locations in the hilly area of Sichuan Province from January to October 2009. A total of 39,2762 individuals were observed, belonging to 146 groups, 7 phyla, 16 classes, and 31 orders. The community composition, trophic group, diversity, and seasonal dynamics of soil fauna in the plantations all varied with slope. The abundance of macro-fauna, xeric meso- and micro-fauna, saprophagous macro-fauna, and omnivorous xeric meso- and micro-fauna increased with the decrease of slope, indicating that soil fauna had sensitive responses to the soil environmental factors affected by slope. Significant differences in the diversity of soil saprophagous macro-fauna and hygrophilous meso- and micro-fauna were observed at different slope locations, suggesting that these two faunal groups could be used as the indicators of the habitat heterogeneity of E. grandis plantations at different slope. Overall, slope location had definite effects on the community structure and distribution of soil fauna in the E. grandis plantations, but the effects were not statistically significant.

  9. Performance of Australian provenances of Eucalyptus grandis and Eucalyptus saligna in Hawaii

    Science.gov (United States)

    Roger G. Skolmen

    1986-01-01

    Australian provenances of Eucalyptus grandis and E. saligna were compared at four locations on the island of Hawaii to seek seed sources better than those in current use which were introduced earlier from unrecorded locations in Australia. A broad range of latitude and elevation was represented among the provenances. At all four...

  10. Above-ground dry matter accumulation by Eucalyptus grandis and its relation to standard meteorological data

    International Nuclear Information System (INIS)

    Byrne, G.F.

    1989-01-01

    The relationship between the rate of increase of biomass in some stands of Eucalyptus grandis, growing near Coffs Harbour, N.S.W., Australia, is explored in terms of estimated evapotranspiration and radiation interception, and related to a similar previous study of Pinus radiata. A possible role of method of planting, site slope and site aspect in biomass increase is also discussed

  11. A Multivariate Study on Genetic Variation in Teak (Tectona grandis (L.))

    DEFF Research Database (Denmark)

    Kjær, Erik Dahl; Siegismund, Hans Redlef; Suangtho, V.

    1996-01-01

    Genetic differentiation between populations of teak (Tectona grandis (L.)) was examined in 9 quantitative characters and 10 allozyme loci. Large differences between populations were revealed in the quantitative traits. Regional patterns were revealed by multivariate analysis of the data, but ther...

  12. High-resolution genetic maps of Eucalyptus improve Eucalyptus grandis genome assembly.

    Science.gov (United States)

    Bartholomé, Jérôme; Mandrou, Eric; Mabiala, André; Jenkins, Jerry; Nabihoudine, Ibouniyamine; Klopp, Christophe; Schmutz, Jeremy; Plomion, Christophe; Gion, Jean-Marc

    2015-06-01

    Genetic maps are key tools in genetic research as they constitute the framework for many applications, such as quantitative trait locus analysis, and support the assembly of genome sequences. The resequencing of the two parents of a cross between Eucalyptus urophylla and Eucalyptus grandis was used to design a single nucleotide polymorphism (SNP) array of 6000 markers evenly distributed along the E. grandis genome. The genotyping of 1025 offspring enabled the construction of two high-resolution genetic maps containing 1832 and 1773 markers with an average marker interval of 0.45 and 0.5 cM for E. grandis and E. urophylla, respectively. The comparison between genetic maps and the reference genome highlighted 85% of collinear regions. A total of 43 noncollinear regions and 13 nonsynthetic regions were detected and corrected in the new genome assembly. This improved version contains 4943 scaffolds totalling 691.3 Mb of which 88.6% were captured by the 11 chromosomes. The mapping data were also used to investigate the effect of population size and number of markers on linkage mapping accuracy. This study provides the most reliable linkage maps for Eucalyptus and version 2.0 of the E. grandis genome. © 2014 CIRAD. New Phytologist © 2014 New Phytologist Trust.

  13. In vitro antioxidant activities of the fractions of Coccinia grandis l. leaf ...

    African Journals Online (AJOL)

    The present study was aimed at investigating the antioxidant activities of the various fractions of the hydromethanolic extract of the leaves of Coccinia grandis L. Voigt. (Cucurbitaceae). The antioxidant activities of the fractions have been evaluated by using nine in vitro assays and were compared to standard antioxidants ...

  14. Establishment of Eucalyptus grandis W. Hill ex Maiden in vitro using ...

    African Journals Online (AJOL)

    Establishment of Eucalyptus grandis W. Hill ex Maiden in vitro using commercial products for seed treatment. Moacir Ribeiro Neto, Cíntia de Oliveira Martendal, Flávia Dionísio Pereira, Edson Luiz Souchie, Fabiano Guimarães Silva ...

  15. Intercropping Acacia mangium stimulates AMF colonization and soil phosphatase activity in Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Daniel Bini

    Full Text Available ABSTRACT: Arbuscular mycorrhizal fungi (AMF are very important to plant nutrition, mostly in terms of acquisition of P and micronutrients. While Acacia mangium is closely associated with AMF throughout the whole cycle, Eucalyptus grandis presents this symbiosis primarily at the seedling stage. The aim of this study was to evaluate the dynamics of AMF in these two tree species in both pure and mixed plantations during the first 20 months after planting. We evaluated the abundance, richness and diversity of AMF spores, the rate of AMF mycorrhizal root colonization, enzymatic activity and soil and litter C, N and P. There was an increase in AMF root colonization of E. grandis when intercropped with A. mangium as well as an increase in the activity of acid and alkaline phosphatase in the presence of leguminous trees. AMF colonization and phosphatase activities were both involved in improvements in P cycling and P nutrition in soil. In addition, P cycling was favored in the intercropped plantation, which showed negative correlation with litter C/N and C/P ratios and positive correlation with soil acid phosphatase activity and soil N and P concentrations. Intercropping A. mangium and E. grandis maximized AMF root colonization of E. grandis and phosphatase activity in the soil, both of which accelerate P cycling and forest performance.

  16. Genetic and environmental factors affecting rooting in Eucalyptus Grandis X Eucalyptus Longistrata hybrid cuttings

    CSIR Research Space (South Africa)

    Naidoo, N

    2012-03-01

    Full Text Available An investigation was undertaken to study the rooting ability of E. grandis x E. longirostrata hybrid cuttings. The plant material was sourced from five families in seedling derived hedges at two nurseries, as well as five families coppiced from...

  17. Diel and seasonal movements by adult Sacramento pikeminnow (Ptychocheilus grandis) in the Eel River, northwestern California

    Science.gov (United States)

    Bret C. Harvey; Rodney J. Nakamoto

    1999-01-01

    Abstract - In late summer and fall, radio-tagged adult Sacramento pike-minnow (Ptychocheilus grandis) at three sites in the Eel River of northwestern California moved more at night than during the day. Fish moved up to 535 m at night and returned to their original positions the following morning. Adult Sacramento pikeminnow at all sites occupied only pools during the...

  18. Tectona grandis L. f (the teak tree; Hindi: Sagallll or Segllll) (~r ...

    Indian Academy of Sciences (India)

    Tectona grandis L. f (the teak tree; Hindi: Sagallll or Segllll) (~r Verhell({Ceae is a large deciduous tree cllitivatedfor its timber. Leaves are large; flmvers are small, white, svveet-scented and borne on highly branched inflorescences. Fruit is hard and enveloped by bladder-like cal.vx. The timber is one of the best for all wood ...

  19. The sphecid wasps (Hym. Ampulicidae, Sphecidae & Crabronidae ...

    African Journals Online (AJOL)

    The list of Egyptian sphecid wasps includes the current names of those species recorded in the literature with indications where these have changed together with previously unrecorded genera and species. Three genera have not been recorded from Egypt hitherto (Spilomena, Crossocerus, Lestica). Twenty-four species ...

  20. Mieren in Veluwebermen: soortenrijkdom en aanbevelingen voor beheer (Hymenoptera: Formicidae).

    NARCIS (Netherlands)

    Noordijk, J.; Boer, P.

    2007-01-01

    Ants in roadside verges on the Veluwe: species richness and recommendations for management (Hymenoptera: Formicidae) Highway verges in the Veluwe region contain some well developed nutrient poor plant communities, like grasslands, grey hair grass vegetation and heather vegetation. These places

  1. New faunistic data on the family Braconidae (Hymenoptera from Korea

    Directory of Open Access Journals (Sweden)

    Tae-Ho An

    2014-12-01

    Full Text Available Twenty-one species of the four genera in the family Braconidae (Hymenoptera are reported for the first time in Korea. These species belong to the subfamily Euphorinae (Braconidae. Faunistic and distribution data are included.

  2. A checklist of Ropalidiini wasps (Hymenoptera: Vespidae: Polistinae in Indochina

    Directory of Open Access Journals (Sweden)

    Pham Phong Huy

    2014-01-01

    Full Text Available As a basis for intensive study of the taxonomy and biogeography of Ropalidiini wasps in Indochina (Hymenoptera: Vespidae: Polistinae, a checklist of Ropalidiini wasps (Hymenoptera: Vespidae is presented. A total of 57 Ropalidiini species and subspecies belonging to three genera from Indochina are listed, together with information of the type material deposited in the Natural History Collection, Ibaraki University, Japan (IUNH and the Institute of Ecology and Biological Resources (IEBR. References of their distribution in Indochina are also provided.

  3. Natural history of interaction between Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae) and its hyperparasitoid Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae).

    Science.gov (United States)

    Sobczak, J F; Maia, D P; Moura, J C M S; Costa, V A; Vasconcellos-Neto, J

    2012-02-01

    Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae) parasitised by Meteorus sp. (Hymenoptera, Braconidae) larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, Pteromalidae). This is the first description of the attack and oviposition of T. albipes.

  4. Dry mass allocation, water use efficiency and delta C-13 in clones of Eucalyptus grandis, E-grandis x camaldulensis and E-grandis x nitens grown under two irrigation regimes

    CSIR Research Space (South Africa)

    Le Roux, D

    1996-05-01

    Full Text Available - cial clones of Eucalyptus grandis W. Hill ex Maiden. implying that less water-use-efficient trees were more productive (Bond and Stock 1990). Similarly, growing season WUE and delta13C were positively correlated in western larch and Eucalyptus globulus... regimes DEBBIE LE ROUX,1,2 WILLIAM D. STOCK,3 WILLIAM J. BOND3 and DAVID MAPHANGA4 1 Division of Forest Science and Technology, CSIR, Pretoria, South Africa 2 Present address: Department of Botany, University of Kansas, Lawrence, KS 66045, USA 3 Department...

  5. Investigation of ESEM/EDX to measure liquor penetration and diffusion in Eucalyptus grandis wood chips during kraft pulping

    CSIR Research Space (South Africa)

    Grzeskowiak, V

    2011-01-01

    Full Text Available Environmental scanning electron microscopy coupled with energy dispersive x-ray (ESEM/EDX) was optimised to measure the penetration and diffusion of cooking liquor into Eucalyptus grandis wood chips during kraft pulping. The moisture content...

  6. Modelos de predição para sobrevivência de plantas de Eucalyptus grandis Prediction models of Eucalyptus grandis plant survival

    Directory of Open Access Journals (Sweden)

    Telde Natel Custódio

    2009-01-01

    Full Text Available Objetivou-se com este trabalho comparar modelos de predição de plantas sobreviventes de Eucalyptus grandis. Utilizaram-se os seguintes modelos: modelo linear misto com os dados transformados, utilizando-se as transformações angular e BOX-COX; modelo linear generalizado misto com distribuição binomial e funções de ligação logística, probit e complemento log-log; modelo linear generalizado misto com distribuição Poisson e função de ligação logarítmica. Os dados são provenientes de um experimento em blocos ao acaso, para avaliação de progênies maternas de Eucalyptus grandis, aos 5 anos de idade, em que a variável resposta são plantas sobreviventes. Para comparação dos efeitos entre os modelos foram estimadas as correlações de Spearman e aplicado o teste de permutação de Fisher. Foi possível concluir que, o modelo linear generalizado misto com distribuição Poisson e função de ligação logarítmica se ajustou mal aos dados e que as estimativas para os efeitos fixos e predição para os efeitos aleatórios, não se diferenciaram entre os demais modelos estudados.The objective of this work was to compare models for prediction of the survival of plants of Eucalyptus grandis. The following models were used: linear mixed model with the transformed data, by utilizing the angular transformations and BOX-COX; generalized linear mixed model with binomial distribution and logistic functions, probit and complement log-log links; generalized linear mixed model with Poisson distribution and logarithmic link function. The data came from a randomized block experiment for evaluation of Eucalyptus grandis maternal progenies at five years old, in which the variable response are surviving plants. For comparison of the effects among the models the correlations of Spearman were estimated and the test of permutation of Fisher was applied. It was possible to conclude that: the generalized linear mixed model with Poisson distribution and

  7. The Composition, Antioxidant and Antibacterial Activities of Cold-Pressed and Distilled Essential Oils of Citrus paradisi and Citrus grandis (L. Osbeck

    Directory of Open Access Journals (Sweden)

    Ming-Chiu Ou

    2015-01-01

    Full Text Available The chemical composition and functional activities of cold-pressed and water distilled peel essential oils of Citrus paradisi (C. paradisi and Citrus grandis (L. Osbeck (C. grandis were investigated in present study. Yields of cold-pressed oils were much higher than those of distilled oils. Limonene was the primary ingredient of essential oils of C. paradisi (cold 92.83%; distilled 96.06% and C. grandis (cold 32.63%; distilled 55.74%. In addition, C. grandis oils obtained were rich in oxygenated or nitrogenated compounds which may be involved in reducing cardiovascular diseases or enhancing sleep effectiveness. The order of free radical scavenging activities of 4 citrus oils was distilled C. paradisi oil > cold-pressed C. paradisi oil > distilled C. grandis oil > cold-pressed C. grandis oil. Cold-pressed C. grandis oil exhibited the lowest activity in all antioxidative assays. The order of antimicrobial activities of 4 citrus oils was distilled C. grandis oil, cold-pressed C. paradisi oil > distilled C. paradisi oil > cold-pressed C. paradisi oil. Surprisingly, distilled C. grandis oil exhibited better antimicrobial activities than distilled C. paradisi oil, especially against Escherichia coli and Salmonella enterica subsp. The results also indicated that the antimicrobial activities of essential oils may not relate to their antioxidative activities.

  8. Temporal dynamics of the response to Al stress in Eucalyptus grandis × Eucalyptus camaldulensis

    Directory of Open Access Journals (Sweden)

    Berenice K. de Alcântara

    2015-06-01

    Full Text Available Lipid peroxidation and root elongation of Eucalyptus grandis × Eucalyptus camaldulensis were studied under stress conditions in response to aluminum (Al, a metal known to limit agricultural productivity in acidic soils primarily due to reduced root elongation. In Brazil, the Grancam 1277 hybrid (E. grandis × E. camaldulensis has been planted in the "Cerrado", a region of the country with a wide occurrence of acidic soils. The present study demonstrated that the hybrid exhibited root growth reduction and increased levels of lipid peroxidation after 24h of treatment with 100 µM of Al, which was followed by a reduction in lipid peroxidation levels and the recovery of root elongation after 48h of Al exposure, suggesting a rapid response to the early stressful conditions induced by Al. The understanding of the temporal dynamics of Al tolerance may be useful for selecting more tolerant genotypes and for identifying genes of interest for applications in bioengineering.

  9. A Newly Identified Passive Hyperaccumulator Eucalyptus grandis × E. urophylla under Manganese Stress.

    Science.gov (United States)

    Xie, Qingqing; Li, Zhenji; Yang, Limin; Lv, Jing; Jobe, Timothy O; Wang, Qiuquan

    2015-01-01

    Manganese (Mn) is an essential micronutrient needed for plant growth and development, but can be toxic to plants in excess amounts. However, some plant species have detoxification mechanisms that allow them to accumulate Mn to levels that are normally toxic, a phenomenon known as hyperaccumulation. These species are excellent candidates for developing a cost-effective remediation strategy for Mn-polluted soils. In this study, we identified a new passive Mn-hyperaccumulator Eucalyptus grandis × E. urophylla during a field survey in southern China in July 2010. This hybrid can accumulate as much as 13,549 mg/kg DW Mn in its leaves. Our results from Scanning Electron Microscope (SEM) X-ray microanalysis indicate that Mn is distributed in the entire leaf and stem cross-section, especially in photosynthetic palisade, spongy mesophyll tissue, and stem xylem vessels. Results from size-exclusion chromatography coupled with ICP-MS (Inductively coupled plasma mass spectrometry) lead us to speculate that Mn associates with relatively high molecular weight proteins and low molecular weight organic acids, including tartaric acid, to avoid Mn toxicity. Our results provide experimental evidence that both proteins and organic acids play important roles in Mn detoxification in Eucalyptus grandis × E. urophylla. The key characteristics of Eucalyptus grandis × E. urophylla are an increased Mn translocation facilitated by transpiration through the xylem to the leaves and further distribution throughout the leaf tissues. Moreover, the Mn-speciation profile obtained for the first time in different cellular organelles of Eucalyptus grandis × E. urophylla suggested that different organelles have differential accumulating abilities and unique mechanisms for Mn-detoxification.

  10. The oropharyngeal morphology in the semiaquatic giant Asian pond turtle, Heosemys grandis, and its evolutionary implications.

    Directory of Open Access Journals (Sweden)

    Monika Lintner

    Full Text Available The oropharynx as a functional entity plays a fundamental role in feeding. Transitions from aquatic to terrestrial lifestyles in vertebrates demanded major changes of the oropharynx for the required adaptations to a different feeding environment. Extant turtles evolved terrestrial feeding modes in three families (testudinids, emydids, geoemydids-independently from other amniotes-and are therefore important model organisms to reconstruct morpho-functional changes behind aquatic-terrestrial transitions. In this study we hypothesized that the oropharyngeal morphology in semiaquatic turtles of the geoemydid family shows parallels to testudinids, the only purely terrestrial extant lineage. We provide an in-depth description of the oropharynx in the semiaquatic geoemydid Heosemys grandis by using a combination of micro computed tomography (micro-CT and subsequent digital in situ 3-D reconstruction, scanning electron microscopy (SEM, and histology. We show that H. grandis has a large tongue with rough papillose surface and well-developed lingual muscles. The attachment sites of the lingual muscles on the hyolingual skeleton and their courses within the tongue are nearly identical with testudinids. The hyolingual skeleton itself is mainly cartilaginous and shows distinct-but compared to testudinids rather small-anterior extensions of the hyoid body and hypoglossum. Oral glands are well developed in H. grandis but are smaller and simpler than in testudinids. Similarly, oropharyngeal keratinization was minimal and found only in the anterior palate, regions close to the beak, and tongue tip. We conclude that H. grandis shows distinct oropharyngeal morpho-functional adaptations for a terrestrial lifestyle but still retains characters typical for aquatic forms. This makes this species an important example showing the oropharyngeal adaptations behind aquatic-terrestrial transitions in turtles.

  11. Intercropping Acacia mangium stimulates AMF colonization and soil phosphatase activity in Eucalyptus grandis

    OpenAIRE

    Bini, Daniel; Santos, Cristiane Alcantara dos; Silva, Mylenne Calcciolari Pinheiro da; Bonfim, Joice Andrade; Cardoso, Elke Jurandy Bran Nogueira

    2018-01-01

    ABSTRACT: Arbuscular mycorrhizal fungi (AMF) are very important to plant nutrition, mostly in terms of acquisition of P and micronutrients. While Acacia mangium is closely associated with AMF throughout the whole cycle, Eucalyptus grandis presents this symbiosis primarily at the seedling stage. The aim of this study was to evaluate the dynamics of AMF in these two tree species in both pure and mixed plantations during the first 20 months after planting. We evaluated the abundance, richness an...

  12. A Newly Identified Passive Hyperaccumulator Eucalyptus grandis × E. urophylla under Manganese Stress.

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    Qingqing Xie

    Full Text Available Manganese (Mn is an essential micronutrient needed for plant growth and development, but can be toxic to plants in excess amounts. However, some plant species have detoxification mechanisms that allow them to accumulate Mn to levels that are normally toxic, a phenomenon known as hyperaccumulation. These species are excellent candidates for developing a cost-effective remediation strategy for Mn-polluted soils. In this study, we identified a new passive Mn-hyperaccumulator Eucalyptus grandis × E. urophylla during a field survey in southern China in July 2010. This hybrid can accumulate as much as 13,549 mg/kg DW Mn in its leaves. Our results from Scanning Electron Microscope (SEM X-ray microanalysis indicate that Mn is distributed in the entire leaf and stem cross-section, especially in photosynthetic palisade, spongy mesophyll tissue, and stem xylem vessels. Results from size-exclusion chromatography coupled with ICP-MS (Inductively coupled plasma mass spectrometry lead us to speculate that Mn associates with relatively high molecular weight proteins and low molecular weight organic acids, including tartaric acid, to avoid Mn toxicity. Our results provide experimental evidence that both proteins and organic acids play important roles in Mn detoxification in Eucalyptus grandis × E. urophylla. The key characteristics of Eucalyptus grandis × E. urophylla are an increased Mn translocation facilitated by transpiration through the xylem to the leaves and further distribution throughout the leaf tissues. Moreover, the Mn-speciation profile obtained for the first time in different cellular organelles of Eucalyptus grandis × E. urophylla suggested that different organelles have differential accumulating abilities and unique mechanisms for Mn-detoxification.

  13. Parasitismo de Palmistichus elaeisis (Hymenoptera: Eulophidae em hospedeiro alternativo sobre plantas de eucalipto em semi-campo Parasitism of Palmistichus elaeisis (Hymenoptera: Eulophidae in alternative host on eucalypt in semi-field

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    Fabrício Fagundes Pereira

    2010-12-01

    Full Text Available O sucesso de programas de controle biológico com parasitóides depende de pesquisas aplicadas e, por isso, estimouse o número ideal de fêmeas de Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera: Eulophidae para liberação em plantios de eucalipto, visando o controle de lepidópteros desfolhadores. Em cada repetição foram utilizadas trinta e seis pupas do hospedeiro alternativo Anticarsia gemmatalis Hübner, 1818 (Lepidoptera: Noctuidae com 24 a 48 horas de idade que, foram individualizadas em armadilhas confeccionadas com tela de náilon e fixadas em ramos de plantas de Eucalyptus grandis nos terços superior, médio e inferior, na proporção de 33,33% (12 armadilhas. Após a fixação das armadilhas, as plantas foram cobertas individualmente, com uma gaiola de 7,0 x 7,0 x 2,5 m confeccionada com tecido organza e posteriormente liberadas, 36; 72; 144; 288; 576 ou 1.152 fêmeas de P. elaeisis, representando um, dois, quatro, oito, 16 ou 32 parasitóides por pupa e na testemunha, não houve liberação do parasitóide. Cada proporção foi considerada um tratamento, sendo instaladas seis repetições permitindo o parasitismo por 96 horas. O número de pupas de A. gemmatalis parasitadas foi crescente com o aumento do número de parasitóides liberados, independentemente do terço da planta considerado, ajustando-se a uma função quadrática com ponto de máximo próximo a 25 fêmeas por pupa. Na densidade de 32 fêmeas/pupa não houve aumento significativo no número de pupas parasitadas, sugerindo 25 fêmeas de P. elaeisis por pupa como a densidade mais próxima do ideal para liberação desse parasitóide em plantios de eucalipto.The success of programs of biological control with parasitoids depends on applied research and therefore it was estimated the optimal number of females Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera: Eulophidae for release in eucalyptus plantations, for the control of lepidopterans

  14. Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis).

    Science.gov (United States)

    Oliveira, Gustavo R; Silva, Maria C M; Lucena, Wagner A; Nakasu, Erich Y T; Firmino, Alexandre A P; Beneventi, Magda A; Souza, Djair S L; Gomes, José E; de Souza, José D A; Rigden, Daniel J; Ramos, Hudson B; Soccol, Carlos R; Grossi-de-Sa, Maria F

    2011-09-09

    The cotton boll weevil (Anthonomus grandis) is a serious insect-pest in the Americas, particularly in Brazil. The use of chemical or biological insect control is not effective against the cotton boll weevil because of its endophytic life style. Therefore, the use of biotechnological tools to produce insect-resistant transgenic plants represents an important strategy to reduce the damage to cotton plants caused by the boll weevil. The present study focuses on the identification of novel molecules that show improved toxicity against the cotton boll weevil. In vitro directed molecular evolution through DNA shuffling and phage display screening was applied to enhance the insecticidal activity of variants of the Cry8Ka1 protein of Bacillus thuringiensis. Bioassays carried out with A. grandis larvae revealed that the LC50 of the screened mutant Cry8Ka5 toxin was 3.15-fold higher than the wild-type Cry8Ka1 toxin. Homology modelling of Cry8Ka1 and the Cry8Ka5 mutant suggested that both proteins retained the typical three-domain Cry family structure. The mutated residues were located mostly in loops and appeared unlikely to interfere with molecular stability. The improved toxicity of the Cry8Ka5 mutant obtained in this study will allow the generation of a transgenic cotton event with improved potential to control A. grandis.

  15. Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis

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    Gomes José E

    2011-09-01

    Full Text Available Abstract Background The cotton boll weevil (Anthonomus grandis is a serious insect-pest in the Americas, particularly in Brazil. The use of chemical or biological insect control is not effective against the cotton boll weevil because of its endophytic life style. Therefore, the use of biotechnological tools to produce insect-resistant transgenic plants represents an important strategy to reduce the damage to cotton plants caused by the boll weevil. The present study focuses on the identification of novel molecules that show improved toxicity against the cotton boll weevil. In vitro directed molecular evolution through DNA shuffling and phage display screening was applied to enhance the insecticidal activity of variants of the Cry8Ka1 protein of Bacillus thuringiensis. Results Bioassays carried out with A. grandis larvae revealed that the LC50 of the screened mutant Cry8Ka5 toxin was 3.15-fold higher than the wild-type Cry8Ka1 toxin. Homology modelling of Cry8Ka1 and the Cry8Ka5 mutant suggested that both proteins retained the typical three-domain Cry family structure. The mutated residues were located mostly in loops and appeared unlikely to interfere with molecular stability. Conclusions The improved toxicity of the Cry8Ka5 mutant obtained in this study will allow the generation of a transgenic cotton event with improved potential to control A. grandis.

  16. Gas Exchange Characteristics in Tectona grandis L. Clones under Varying Concentrations of CO2 Levels

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    S. Saravanan

    2014-08-01

    Full Text Available The Institute of Forest Genetics and Tree Breeding, Coimbatore, India functioning under the Indian Council of Forestry Research and Education, Dehara Dun, has a long term systematic tree improvement program for Tectona grandis aimed to enhancing productivity and screening of clones for site specific. In the process, twenty clones of T. grandis L. were studied for the physiological parameters and water use efficiency with reference to the elevated CO2 levels. CO2 enrichment studies in special chambers help in understanding the changes at individual level, and also at physiological, biochemical and genetic level. It also provides valuable information for establishing plantations at different geographic locations. Considerable variations were observed when the selected 20 clones of T. grandis were subjected to physiological studies under elevated CO2 conditions (600 and 900 mol mol-1. Eight clones exhibited superior growth coupled with favorable physiological characteristics including high photosynthetic rate, carboxylation and water use efficiency under elevated CO2 levels. Clones with minimal variation in physiological characteristics under elevated levels of CO2 suggest their ability to overcome physiological stresses and adapt to varying climatic conditions.

  17. Development and assessment of microarray-based DNA fingerprinting in Eucalyptus grandis.

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    Lezar, Sabine; Myburg, A A; Berger, D K; Wingfield, M J; Wingfield, B D

    2004-11-01

    Development of improved Eucalyptus genotypes involves the routine identification of breeding stock and superior clones. Currently, microsatellites and random amplified polymorphic DNA markers are the most widely used DNA-based techniques for fingerprinting of these trees. While these techniques have provided rapid and powerful fingerprinting assays, they are constrained by their reliance on gel or capillary electrophoresis, and therefore, relatively low throughput of fragment analysis. In contrast, recently developed microarray technology holds the promise of parallel analysis of thousands of markers in plant genomes. The aim of this study was to develop a DNA fingerprinting chip for Eucalyptus grandis and to investigate its usefulness for fingerprinting of eucalypt trees. A prototype chip was prepared using a partial genomic library from total genomic DNA of 23 E. grandis trees, of which 22 were full siblings. A total of 384 cloned genomic fragments were individually amplified and arrayed onto glass slides. DNA fingerprints were obtained for 17 individuals by hybridizing labeled genome representations of the individual trees to the 384-element chip. Polymorphic DNA fragments were identified by evaluating the binary distribution of their background-corrected signal intensities across full-sib individuals. Among 384 DNA fragments on the chip, 104 (27%) were found to be polymorphic. Hybridization of these polymorphic fragments was highly repeatable (R2>0.91) within the E. grandis individuals, and they allowed us to identify all 17 full-sib individuals. Our results suggest that DNA microarrays can be used to effectively fingerprint large numbers of closely related Eucalyptus trees.

  18. Evaluation of Chemical Composition and Biological Activities of Citrus pseudolimon and Citrus grandis Peel Essential Oils

    International Nuclear Information System (INIS)

    Sajid, A.; Hanif, M.A.; Shahid, M.

    2016-01-01

    Essential oils and their volatile constituents are used extensively to prevent and treat human diseases. In the past decades, worldwide demand for citrus essential oils has greatly increased. Citrus essential oils containing 85-99 percent volatile and 1-15 percent non-volatile components. Essential oils from Citrus pseudolimon and Citrus grandis peels were extracted through steam distillation and characterized by GC-MS. C. pseudolimon has thirty six and C. grandis has thirty three total components; limonene 47.07 percent and 71.48 percent was the major component in both oils respectively. Antioxidant activity was checked by 2, 2-diphenyl-1-picrylhydrazyl radical assay and β-carotene/linoleic acid bleaching test. Both oils have modest activity. The antimicrobial potential was assessed against different bacterial and fungus strains. C. pseudolimon oil possessed strong activity against all tested strains while C. grandis has moderate activity. The antitumor activity was evaluated by potato disc assay, C. pseudolimon showed 81.25 inhibition. Hence the essential oils could have a great potential in pharmaceutical industry. (author)

  19. MANEJO DO SOLO E CRESCIMENTO INICIAL DE Eucalyptus grandis Hill ex Maiden EM ARGISSOLO

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    Juliana Prevedello

    2013-01-01

    Full Text Available The forest species cultivation with rapid growth in Brazil has increased, mainly due to the diverse use of its wood and climate adaptation. The cultivation with minimum tillage in the forest sector stands out a way to increase productivity, combined with the maintenance of biodiversity and soil conservation. This study was conducted at the experimental area of State Foundation for Agricultural Research - Research Center for Forestry in Santa Maria - RS, with aimed to evaluate the effect of the soil tillage methods on soil physical properties and on initial development of Eucalyptus grandis, in a sandy loam Typic Hapludalf. Four soil management practices were compared: no-tillage; chisel tillage; chisel tillage plus harrowing and; rotary tillage (rotary tiller, installed in a randomized block design with three replications. The soil under no-tillage conditioned lower initial growth of eucalyptus due higher soil penetration resistance and bulk density, when compared with treatments with mobilization. The root distribution analysis in soil, despite being a qualitative method, was effective in demonstrating the effect of soil tillage for the Eucalyptus grandis plantation. The soil tillage with mobilization resulted in a higher initial growth of Eucalyptus grandis. The chisel tillage effects in the soil physical properties persisted after one year of soil tillage.

  20. EFFECT OF FERTILIZATION ON MECHANICAL PROPERTIES OF THE WOOD OF Eucalyptus grandis

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    Israel Luiz de Lima

    2011-09-01

    Full Text Available The effect of the fertilization in the amount and quality of the produced wood is one of the questions to be considered in the research of the Eucalyptus grandis. The present work aimed to evaluate the fertilization effect in the mechanical properties of Eucalyptus grandis. The population of Eucalyptus grandis was 21 years old and was managed under the system of selective thinning, with application of fertilizers. The factors used in this study were: presence or absence of fertilizers, two positions of log and five radial positions. The influences of the factors and of their combinations were evaluated regarding to compression strength, shear strength, modulus of rupture and modulus of elasticity in static banding. The compressive strength and the modulus of elasticity had been influenced by the factors: fertilizer and radial positions of the log. There was also an increase in the direction of the pith-bark in all studied properties. A good positive relationship was found to exist among the compression strength, the shear, the modulus of rupture and the modulus of elasticity with radial position.

  1. Ovarian egg morphology in chalcidoid wasps (Hymenoptera: Chalcidoidea parasitizing gall wasps (Hymenoptera: Cynipidae

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    Vårdal, H.

    2016-06-01

    Full Text Available We provide morphological egg data of 26 species of 5 chalcidoid families associated with cynipid galls (Hymenoptera: Cynipidae from western Palaearctic, including the first egg data for the family Ormyridae. Adult chalcidoid species were reared from galls, and eggs obtained from dissected female ovaries were examined using scanning electron microscopy (SEM. The shape of the eggs varies from oval to elongate and tapered at both ends. Eggs of Eurytomidae as well as some Eulophidae, Eupelmidae and Pteromalidae are equipped with a peduncle at the anterior end. We found a positive correlation between long eggs and long ovipositors and confirmed the expectation that eggs of endoparasitoids are generally shorter and narrower than eggs of ectoparasitoids. We were able to locate the sperm entrance or micropyle at the anterior pole of eggs of several species. It is situated at the anterior end of the egg and at the end of the peduncle when present. In addition, the eggshells of the endoparasitoid Sycophila biguttata (Swederus, 1795 (Hymenoptera: Eurytomidae and the ectoparasitoid Cecidostiba fungosa (Geoffroy, 1785 (Hymenoptera: Pteromalidae, are for the first time described.En el presente trabajo se aportan datos morfol.gicos del huevo de 26 especies del Paleártico occidental pertenecientes a 5 familias de Chalcidoidea asociadas con agallas de cinípidos (Hymenoptera: Cynipidae, incluyendo los primeros datos del huevo de especies de Ormyridae. Los ejemplares adultos de las especies estudiadas fueron obtenidos por emergencia de agallas en laboratorio, los ovarios de las hembras diseccionados para obtener los huevos, que fueron finalmente estudiados utilizando técnicas de microscopía electronica de barrido. La forma de los huevos estudiados varía de ovalada a alargada y ahusada en ambos extremos. Los huevos de Eurytomidae, así como algunos de Eulophidae, Eupelmidae y Pteromalidae están provistos de un pedúnculo en el extremo anterior. Se encontr

  2. Comparisons between two economically valuable forest species (Eucalyptus grandis and Pinus taeda in relation to seed behaviour under controlled deterioration

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    Jussara Bertho Fantinatti

    2011-02-01

    Full Text Available The objectives of this work were to analyze seed behaviour under controlled deterioration and estimate viability equations for forest species Eucalyptus grandis and Pinus taeda. Desired moisture content levels were achieved from initial values after either rehydration over water or drying over silica gel, both at 25 ºC. Seed sub samples with 8 moisture contents each for E. grandis (1.2 to 18.1%, initial value of 11.3% and P. taeda (1.5 to 19.5%, initial value of 12.9% were sealed in laminate aluminium-foil packets and stored in incubators maintained at 40, 50 and 65 ºC. The seeds from these species exhibited true orthodox and sub-orthodox storage behaviour, respectively, however E. grandis showed higher seed storability, probably due to a different seed chemical composition. Lowest moisture content limits estimated for application of the viability equations at 65 ºC were 4.9 and 4.1 mc for E. grandis and P. taeda, on equilibrium with ±20% RH. The viability equation estimated quantified the response of seed longevity to storage environment well with K E = 9.661 and 8.838; C W = 6.467 and 5.981; C H = 0.03498 and 0.10340; C Q = 0.0002330 and 0.0005476, for E. grandis and P. taeda, respectively.

  3. Recombinant Cry1Ia protein is highly toxic to cotton boll weevil (Anthonomus grandis Boheman) and fall armyworm (Spodoptera frugiperda).

    Science.gov (United States)

    Martins, E S; Aguiar, R W D S; Martins, N F; Melatti, V M; Falcão, R; Gomes, A C M M; Ribeiro, B M; Monnerat, R G

    2008-05-01

    To evaluate the activity of cry1Ia gene against cotton pests, Spodoptera frugiperda and Anthonomus grandis. Had isolated and characterized a toxin gene from the Bacillus thuringiensis S1451 strain which have been previously shown to be toxic to S. frugiperda and A. grandis. The toxin gene (cry1Ia) was amplified by PCR, sequenced, and cloned into the genome of a baculovirus. The Cry1Ia protein was expressed in baculovirus infected insect cells, producing protein inclusions in infected cells. The Cry1Ia protein has used in bioassays against to S. frugiperda and A. grandis. Bioassays using the purified recombinant protein showed high toxicity to S. frugiperda and A. grandis larvae. Molecular modelling of the Cry1Ia protein translated from the DNA sequence obtained in this work, showed that this protein possibly posses a similar structure to the Cry3A protein. Ultrastructural analysis of midgut cells from A. grandis incubated with the Cry1Ia toxin, showed loss of microvilli integrity. The results indicate that the cry1Ia is a good candidate for the construction of transgenic plants resistant to these important cotton pests.

  4. MODIFICACIÓN AL PROCESO DE PRODUCCIÓN MASIVA DE LOS PARASITOIDES Catolaccus grandis Y Catolaccus hunteri

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    Marco Antonio Reyes-Rosas

    2010-01-01

    Full Text Available El presente estudio se realizó con el objetivo de modificar la tecnología de producción masiva de Catolaccus grandis y Catolaccus hunteri, himenópteros parasitoides de Anthonomus grandis el picudo del algodonero en Tamaulipas, México en el 2002. Se determinó la eficiencia del papel toalla para el secado de manos, en el sellamiento de cápsulas moldeadas en cera de hidrocarburos y poliolefina (LCHP, donde se confina al huésped, y evaluar la efectividad del gorgojo del garbanzo Callosobruchus maculatus como hospedante facticio para la cría de ambos parasitoides. Hubo producción masiva de C. grandis y C. hunteri utilizando C. maculatus como huésped y el papel toalla fue efectivo para sellar los orificios de las cápsulas moldeadas en LCHP. La producción masiva de C. grandis y C. hunteri mediante C. maculatus, contribuyó a incrementar la seguridad y facilidad del proceso, además de una significativa reducción de costos. El papel toalla en el sellado de los orificios de las cápsulas moldeadas en LCHP, redujo en un 50% por m2 los costos del proceso, sin afectar la eficiencia del parasitismo y emergencia de C. grandis.

  5. Head capsule characters in the Hymenoptera and their phylogenetic implications

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    Lars Vilhelmsen

    2011-09-01

    Full Text Available The head capsule of a taxon sample of three outgroup and 86 ingroup taxa is examined for characters of possible phylogenetic significance within Hymenoptera. 21 morphological characters are illustrated and scored, and their character evolution explored by mapping them onto a phylogeny recently produced from a large morphological data set. Many of the characters are informative and display unambiguous changes. Most of the character support demonstrated is supportive at the superfamily or family level. In contrast, only few characters corroborate deeper nodes in the phylogeny of Hymenoptera.

  6. Primera cita de la Argentina de Ophelimus maskelli (Ashmead (Hymenoptera: Eulophidae y su parasitoide, Closterocerus chamaeleon (Girault (Hymenoptera: Eulophidae

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    Daniel A AQUINO

    2014-01-01

    Full Text Available Se cita por primera vez de la Argentina la presencia de la avispa galí - cola exótica Ophelimus maskelli (Ashmead (Hymenoptera: Eulophidae y su para - sitoide natural, Closterocerus chamaeleon (Girault (Hymenoptera: Eulophidae en Eucalyptus camaldulensis de la provincia de Buenos Aires. Ophelimus maskelli es una especie invasora de origen Australiano que ha sido reportada como plaga de los eucaliptos en numerosos países de Asia, Europa y América. Closterocerus chamaleon es un parasitoide exótico cuyo potencial como biocontrolador de O. maskelli está siendo evaluado en algunos países.

  7. Natural history of interaction between Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae and its hyperparasitoid Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae

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    JF Sobczak

    Full Text Available Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae parasitised by Meteorus sp. (Hymenoptera, Braconidae larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, Pteromalidae. This is the first description of the attack and oviposition of T. albipes.

  8. Effects of autohydrolysis of Eucalyptus urograndis and Eucalyptus grandis on influence of chemical components and crystallinity index.

    Science.gov (United States)

    da Silva Morais, Alaine Patrícia; Sansígolo, Cláudio Angeli; de Oliveira Neto, Mario

    2016-08-01

    Samples of Eucalyptus urograndis and Eucalyptus grandis sawdust were autohydrolyzed in aqueous conditions to reach temperatures in the range 110-190°C and reaction times of 0-150min in a minireactor. In each minireactor were used a liquor:wood ratio (10:1 L:kg dry wood), in order to assess the effects of the autohydrolysis severity and the crystalline properties of cellulose. The content of extractives, lignin, holocellulose, cellulose, hemicelluloses and crystallinity index obtained from the solid fraction after autohydrolysis of sawdust were determined. This study demonstrated that the hemicelluloses were extensively removed at 170 and 190°C, whereas cellulose was partly degraded to Eucalyptus urograndis and Eucalyptus grandis sawdust. The lignin content decreased, while the extractives content increased. It was defined that during autohydrolysis, had a slight decreased on crystalline structure of cellulose of Eucalyptus urogandis and Eucalyptus grandis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Tolerance of Anadenanthera peregrina to Eucalyptus camaldulensis and Eucalyptus grandis essential oil as condition for mixed plantation

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    Neimar de Freitas Duarte

    2012-06-01

    Full Text Available With the purpose of selecting the species of woody Caatinga for mixed plantations with Eucalyptus spp., the allelophatic effects of E. camaldulensis and E. grandis essential oil were studied on the growth activities of Anadenanthera peregrina. The plants were closed in glass chambers in the presence of volatile oil of E. camaldulensis or E. grandis at the concentration of 13 nl.cm-3. The number of leaves, height and diameter at soil lever were compared before, immediately after and after 30 days. Chlorophyll a and b, carotenoids and dry mass were evaluated after the treatment application. There was no inhibitory effect of E. camaldulensis and E. grandis oils on A. peregrina. E. camaldulensis, which was more adapted to semi-arid conditions, was planted in mixture stands with two native legume species, inoculated with Rhizobium and arbuscular mycorrhizal fungi. E. camaldulensis did not inhibit native species growth after two years of cultivation.

  10. Hymenoptera stings in Brazil: a neglected health threat in Amazonas State

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    Allyson Guimarães Costa

    Full Text Available Abstract INTRODUCTION: Hymenoptera injuries are commonly caused by stinging insects. In Amazonas state, Brazil, there is no information regarding distribution, profile, and systemic manifestations associated with Hymenoptera injuries. METHODS: This study aimed to identify risk factors for systemic manifestation using the Brazilian Notifiable Diseases Surveillance System (2007 to 2015. RESULTS: Half of Hymenoptera injuries were caused by bee stings. Hymenoptera injuries were concentrated in Manaus, and 13.36% of cases displayed systemic signs. Delayed medical assistance (4 to 12 hours presented four times more risk for systemic manifestations. CONCLUSIONS: Simple clinical observations and history of injury are critical information for prognostic improvement.

  11. Evaluation of Antibacterial Activities of Citrus limon, Citrus reticulata, and Citrus grandis Against Pathogenic Bacteria

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    Sholeh Saeb

    2016-11-01

    Full Text Available Background: Microorganisms resistant to most antibiotics are rapidly spreading, and there is an urgent and continuous need for novel antimicrobial compounds. The genus Citrus belongs to the family Rutaceae has many biologically active secondary metabolites. Objectives: The purpose of this study was to evaluate antimicrobial activity of essential oil and extract of Lemon (Citrus limon, Mandarin (Citrus reticulata and Pummelo (Citrus grandis against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella typhi. Materials and Methods: The fresh Citrus leaves were shade-dried and powdered. Antimicrobial metabolites were extracted from them by 80% methanol for extract and using a Clevenger-type apparatus for essential oil. Eight different concentrations of the each leaf extract and essential oil were prepared. The antimicrobial susceptibility assay of Citrus leaves metabolites were subjected against four bacterial strains by agar disc diffusion and E-test method. Results: In this study, minimum inhibitory concentrations (MIC of different Citrus leaf extracts were determined against all four food-borne pathogens. The C. grandis leaf essential oil had potent antimicrobial activity against all four pathogens, and the C. limon leaf essential oil was effective on Gram-positive bacteria. S. typhi was resistant against two leaves essential oils. Conclusions: The results showed that there was no antimicrobial activity effect in all extracts on tested bacteria. In this study, the antibacterial effect of essential oil of Citrus leaves on four strains of pathogenic microorganisms was confirmed. The C. grandis leaf essential oil had the most powerful antimicrobial properties, suggesting its potential application as natural preservative in foods or an effective medicine against different pathogenic microbes. Key words: Antibacterial activity, E-test, Citr

  12. Lufenuron impact upon Anthonomus grandis Boheman (Coleoptera: Curculionidae) midgut and its reflection in gametogenesis.

    Science.gov (United States)

    Costa, Hilton Nobre; da Cunha, Franklin Magliano; Cruz, Glaucilane Santos; D'assunção, Carolline Guimarães; Rolim, Guilherme Gomes; Barros, Maria Edna Gomes; Breda, Mariana Oliveira; Teixeira, Alvaro Aguiar Coelho; Teixeira, Valéria Wanderley

    2017-04-01

    The insecticide Match® (lufenuron), one of the main insect growth regulators used in pest control, has been presented as a viable alternative against the boll weevil, Anthonomus grandis (Coleoptera: Curculionidae), by inhibiting chitin synthesis. Thus, this study aimed to examine whether Match® interferes in the synthesis of the peritrophic matrix, leading to changes in the midgut epithelium, resulting in nutritional deficiency and reflecting, thereby, in the gametogenesis process of A. grandis. Floral cotton buds were immersed in the insecticide solution (800μL of Match®+200mL of distilled water) and offered to the adult insects. The midguts of the insects were evaluated after 24 and 120h after feeding. The gonads were evaluated after 120h. The results showed that Match®, in both evaluation periods, induced histopathological alterations such as disorganization, vacuolization and desquamation of the midgut epithelium; histochemical modifications in the distribution patterns of carbohydrates, although without quantitative changes; and a strong decrease in protein levels. No apoptosis were observed, however, there was an increase in the number of regenerative cell nests. In the testicles, a reduction in the amount of spermatozoids and reduced carbohydrate levels were observed, but no difference in protein levels. The ovarioles presented structural disorganization of follicular cells, yolk reduction and decrease in protein levels, however, no change in carbohydrates levels was noted. Therefore, it is concluded that Match® performs histopathologic and histochemical alterations in the midgut epithelium and the gonads of A. grandis adults, reflecting in the gametogenesis process, presenting itself as a promising tool in the management of this pest on cotton crops. Copyright © 2016 Elsevier Inc. All rights reserved.

  13. Toxicity to cotton boll weevil Anthonomus grandis of a trypsin inhibitor from chickpea seeds.

    Science.gov (United States)

    de P G Gomes, Angélica; Dias, Simoni C; Bloch, Carlos; Melo, Francislete R; Furtado, José R; Monnerat, Rose G; Grossi-de-Sá, Maria F; Franco, Octávio L

    2005-02-01

    Cotton (Gossypium hirsutum L.) is an important agricultural commodity, which is attacked by several pests such as the cotton boll weevil Anthonomus grandis. Adult A. grandis feed on fruits and leaf petioles, reducing drastically the crop production. The predominance of boll weevil digestive serine proteinases has motivated inhibitor screenings in order to discover new ones with the capability to reduce the digestion process. The present study describes a novel proteinase inhibitor from chickpea seeds (Cicer arietinum L.) and its effects against A. grandis. This inhibitor, named CaTI, was purified by using affinity Red-Sepharose Cl-6B chromatography, followed by reversed-phase HPLC (Vydac C18-TP). SDS-PAGE and MALDI-TOF analyses, showed a unique monomeric protein with a mass of 12,877 Da. Purified CaTI showed significant inhibitory activity against larval cotton boll weevil serine proteinases (78%) and against bovine pancreatic trypsin (73%), when analyzed by fluorimetric assays. Although the molecular mass of CaTI corresponded to alpha-amylase/trypsin bifunctional inhibitors masses, no inhibitory activity against insect and mammalian alpha-amylases was observed. In order to observe CaTI in vivo effects, an inhibitor rich fraction was added to an artificial diet at different concentrations. At 1.5% (w/w), CaTI caused severe development delay, several deformities and a mortality rate of approximately 45%. These results suggested that CaTI could be useful in the production of transgenic cotton plants with enhanced resistance toward cotton boll weevil.

  14. Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis.

    Science.gov (United States)

    Almeida Garcia, Rayssa; Lima Pepino Macedo, Leonardo; Cabral do Nascimento, Danila; Gillet, François-Xavier; Moreira-Pinto, Clidia Eduarda; Faheem, Muhammad; Moreschi Basso, Angelina Maria; Mattar Silva, Maria Cristina; Grossi-de-Sa, Maria Fatima

    2017-01-01

    RNA interference (RNAi) approaches have been applied as a biotechnological tool for controlling plant insect pests via selective gene down regulation. However, the inefficiency of RNAi mechanism in insects is associated with several barriers, including dsRNA delivery and uptake by the cell, dsRNA interaction with the cellular membrane receptor and dsRNA exposure to insect gut nucleases during feeding. The cotton boll weevil (Anthonomus grandis) is a coleopteran in which RNAi-mediated gene silencing does not function efficiently through dsRNA feeding, and the factors involved in the mechanism remain unknown. Herein, we identified three nucleases in the cotton boll weevil transcriptome denoted AgraNuc1, AgraNuc2, and AgraNuc3, and the influences of these nucleases on the gene silencing of A. grandis chitin synthase II (AgraChSII) were evaluated through oral dsRNA feeding trials. A phylogenetic analysis showed that all three nucleases share high similarity with the DNA/RNA non-specific endonuclease family of other insects. These nucleases were found to be mainly expressed in the posterior midgut region of the insect. Two days after nuclease RNAi-mediated gene silencing, dsRNA degradation by the gut juice was substantially reduced. Notably, after nucleases gene silencing, the orally delivered dsRNA against the AgraChSII gene resulted in improved gene silencing efficiency when compared to the control (non-silenced nucleases). The data presented here demonstrates that A. grandis midgut nucleases are effectively one of the main barriers to dsRNA delivery and emphasize the need to develop novel RNAi delivery strategies focusing on protecting the dsRNA from gut nucleases and enhancing its oral delivery and uptake to crop insect pests.

  15. [Distribution pattern of meso-micro soil fauna in Eucalyptus grandis plantation].

    Science.gov (United States)

    Huang, Yumei; Zhang, Jian; Yang, Wanqin

    2006-12-01

    In this paper, meso-micro soil fauna were extracted and collected by Baermann's and Tullgren' s method, and their distribution pattern in the Eucalyptus grandis plantation of Hongya County, Sichuan Province was studied. A total of 13 550 specimens were collected, belonging to 6 phyla, 13 classes, and 26 orders. Acarina, Nematoda, Collembola were the dominant groups, and Enchytraeidae was the frequent one. The group and individual numbers of meso-micro soil fauna varied with seasons, being the maximum in autumn or winter, fewer in summer, and the minimum in spring. The density of meso-micro soil fauna in soil profile decreased rapidly with increasing soil depth, but a converse distribution was observed from time to time in 5 - 10 cm and 10 - 15 cm soil layers. The meso-micro soil fauna collected by Baermann's and Tullgren's method had a density of 3. 333 x 10(3) - 2. 533 x 10(5) ind x m(-2) and 1.670 x 10(2) - 2.393 x 10(5) ind x m(-2), respectively, and the decreasing rate of the density with the increase of soil depth was higher for those collected by Tullgren's method. The density-group index of meso-micro soil fauna in the E. grandis plantation was the lowest in spring, but the highest in autumn or summer. There were no significant differences in the density of meso-micro soil fauna and in the density-group index between E. grandis plantation and Quercus acutissima secondary forest.

  16. Green synthesis of silver nanoparticles mediated by Coccinia grandis and Phyllanthus emblica: a comparative comprehension

    Science.gov (United States)

    Nayagam, Vasanth; Gabriel, Melchias; Palanisamy, Kumaravel

    2018-04-01

    Fruit extracts also have the potentiality to synthesize silver nanoparticles, which serve as antimicrobial agent in the biological field. At present, the field of biomedical largely depends on the biosynthesized NPs to fight against the multi-drug-resistant pathogens. The fruit residue of Coccinia grandis and Phyllanthus emblica are employed for synthesizing AgNPs by green method. The NPs are further subjected to UV, FTIR, SEM, and XRD measurements. The ten different pathogens were tested against the AgNPs synthesized. The same were tested for early growth of some seed variety too, so as to check the advantages of AgNPs. The UV spectrum analysis showed 442 nm and 423 nm, respectively, and FTIR peaks for the functional group that is responsible for the conversion of NPs were observed at 1640.02 for N-H bond amines (Coccinia grandis) and at 1637.45 for N-H bond amines (Phyllanthus emblica). The SEM results also illustrated that AgNPs are spherical in shape. The XRD patterns indicate the crystalline nature of the AgNPs formed with both these plants. The antimicrobial assay of AgNPs from Coccinia grandis shows maximum zone of inhibition (14 mm) for Vibrio cholerae whereas the AgNPs from Phyllanthus emblica show maximum inhibition at distinct points, namely for Staphylococcus aureus, Vibrio cholerae, Salmonella typhi, and Proteus mirabilis (12 mm). Seed germination initiated by AgNPs is quiet effective and healthier compared to the water-induced seeds. Hence, biogenic AgNPs have various applications in favour of human society.

  17. Sequential Sampling Plan of Anthonomus grandis (Coleoptera: Curculionidae) in Cotton Plants.

    Science.gov (United States)

    Grigolli, J F J; Souza, L A; Mota, T A; Fernandes, M G; Busoli, A C

    2017-04-01

    The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is one of the most important pests of cotton production worldwide. The objective of this work was to develop a sequential sampling plan for the boll weevil. The studies were conducted in Maracaju, MS, Brazil, in two seasons with cotton cultivar FM 993. A 10,000-m2 area of cotton was subdivided into 100 of 10- by 10-m plots, and five plants per plot were evaluated weekly, recording the number of squares with feeding + oviposition punctures of A. grandis in each plant. A sequential sampling plan by the maximum likelihood ratio test was developed, using a 10% threshold level of squares attacked. A 5% security level was adopted for the elaboration of the sequential sampling plan. The type I and type II error used was 0.05, recommended for studies with insects. The adjustment of the frequency distributions used were divided into two phases, so that the model that best fit to the data was the negative binomial distribution up to 85 DAE (Phase I), and from there the best fit was Poisson distribution (Phase II). The equations that define the decision-making for Phase I are S0 = -5.1743 + 0.5730N and S1 = 5.1743 + 0.5730N, and for the Phase II are S0 = -4.2479 + 0.5771N and S1 = 4.2479 + 0.5771N. The sequential sampling plan developed indicated the maximum number of sample units expected for decision-making is ∼39 and 31 samples for Phases I and II, respectively. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. Antraquinonas e naftoquinonas do caule de um espécime de reflorestamento de Tectona grandi (Verbenaceae)

    OpenAIRE

    Moreira,Rafael Y.O.; Arruda,Mara S.P.; Arruda,Alberto C.; Santos,Lourivaldo S.; Müller,Adolfo H.; Guilhon,Giselle M.S.P.; Santos,Alberdan S.; Terezo,Evaristo

    2006-01-01

    O fracionamento do extrato hexânico do caule de um espécime de reflorestamento de Tectona grandis (Verbenaceae), através de procedimentos fitoquímicos clássicos, levou ao isolamento das naftoquinonas lapachol e desidro-a-lapachona e das antraquinonas tectoquinona e obtusifolina. As estruturas das substâncias foram caracterizadas através da análise de métodos espectrométricos de RMN. Este é o primeiro estudo fitoquímico de um espécime de reflorestamento de Tectona grandis, no Brasil, sendo o o...

  19. A preliminary checklist of the ants (Hymenoptera: Formicidae) of ...

    African Journals Online (AJOL)

    A preliminary species checklist of the ants (Hymenoptera: Formicidae) of. Kakamega Forest, Western Kenya, is presented. The species list is based on specimens sampled from 1999 until 2009, which are deposited in the ant collection of the Zoological Research Museum Koenig, Bonn, Germany, and the Natural History ...

  20. Six new genera of Braconidae (Hymenoptera) from China

    NARCIS (Netherlands)

    Achterberg, van C.; Chen, X.

    2004-01-01

    Six new genera of the family Braconidae (Hymenoptera) from China are described and illustrated: two genera of the subfamily Agathidinae: Facilagathis gen. nov. (type species: F. spinulata spec. nov.) and Cremnoptoides gen. nov. (type species: Cremnops pappi Sharkey, 1994); one genus of the subfamily

  1. On the Hymenoptera (exclusive of the Anthophila and Formicidae)

    NARCIS (Netherlands)

    Cameron, P.

    1913-01-01

    Our knowledge of the Hymenoptera of the Island of Waigeu hitherto has been based on the material collected by Dr. A. R. Wallace and described by Mr. Frederick Smith in the Journal of the Linnean Society of London, 1863, pp. 6—48. Omitting the bees (5 species) and the ants (27 species) Smith

  2. An anocellar polistine wasp (Hymenoptera, Vespidae, Polistinae from Texas

    Directory of Open Access Journals (Sweden)

    Volker Lohrmann

    2016-12-01

    Full Text Available A remarkable teratological female of Polistes (Fuscopolistes dorsalis neotropicus Bequaert, 1940 (Vespidae: Polistinae is described and illustrated. The specimen lacks all three external dorsal ocelli but is normally developed in almost every other aspect. Additionally, similar findings in other Hymenoptera are briefly discussed, as are the consequences and the reasons that might cause the random loss of ocelli.

  3. Provisional host catalogue of Fig wasps (Hymenoptera, Chalcidoidea)

    NARCIS (Netherlands)

    Wiebes, J.T.

    1966-01-01

    INTRODUCTION In this catalogue — entitled "provisional" because our knowledge of the subject is still so evidently incomplete — all species of Ficus mentioned as hosts of fig wasps, are listed with the Hymenoptera Chalcidoidea reared from their receptacles. The names used for the Agaonidae are in

  4. De urntjeswesp Eumenes coronatus zoekt het hogerop (Hymenoptera: Vespidae)

    NARCIS (Netherlands)

    Smit, J.

    2005-01-01

    The potter wasp Eumenes coronatus on the move (Hymenoptera: Vespidae) The first record of Eumenus coronatus in the Netherlands dates back from 1954. After that the species was not recorded until 1988. Since then many specimens were collected in the southern part of the country. In 2004 a female was

  5. Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera ...

    African Journals Online (AJOL)

    Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera) pollination on yields of Cucumeropsis mannii (Naudin) in Kisangani, Democratic Republic of Congo. Boniface Posho Ndola, Yves Brostaux, Guillaume Le Goff, Marie-Lucie Susini, Eric Haubruge, Frederic Francis, Bach Kim Nguyen ...

  6. Revision of the world species of Xeris Costa (Hymenoptera: Siricidae)

    Science.gov (United States)

    Henri Goulet; Caroline Boudreault; Nathan M. Schiff

    2015-01-01

    Xeris is one of ten extant genera of Siricidae known as as woodwasps or horntails. They are important wood-boring Hymenoptera from the Northern Hemisphere. Adults and larvae of Xeris are often intercepted at ports and are consequently of concern as potential alien invasive species. The genus consists of 16 species with eight in...

  7. In-vitro diagnostics of Hymenoptera venom allergy

    NARCIS (Netherlands)

    Rueff, F.; Vos, B.; Przybilla, B.

    In-vitro diagnostics of Hymenoptera venom allergy Patients with a history of anaphylactic sting reactions require an allergological work-up (history, in-vitro tests, and skin tests) to clarify indications on venom immunotherapy and on the type of venom to be used. To demonstrate a venom

  8. Field response of predatorRhizophagus grandis to prey frass and synthetic attractants.

    Science.gov (United States)

    Wainhouse, D; Beech-Garwood, P A; Howell, R S; Kelly, D; Orozco, M P

    1992-10-01

    A lure based on the proportional composition of monoterpenes inD. micans larval frass and deployed in Theysohn slot traps was highly attractive toR. grandis released in the field. The relative response to frass and lure was consistent over a range of doses, and behavior close to traps baited with either lure or frass appeared to be similar. The monoterpenes, formulated with antioxidant, appear to be stable over several weeks when released from proprietary reservoir and wick "air fresheners." The lure may be of value in monitoring predator populations.

  9. Analysis of Cry8Ka5-binding proteins from Anthonomus grandis (Coleoptera: Curculionidae) midgut.

    Science.gov (United States)

    Nakasu, Erich Y T; Firmino, Alexandre A P; Dias, Simoni C; Rocha, Thales L; Ramos, Hudson B; Oliveira, Gustavo R; Lucena, Wagner; Carlini, Célia R; Grossi-de-Sá, Maria Fátima

    2010-07-01

    Biotech crops expressing Bacillus thuringiensis Cry toxins present a valuable approach for insect control. Cry8Ka5, which is highly toxic to the cotton boll weevil (Anthonomus grandis), was used as a model to study toxin-ligand interactions. Three Cry-binding proteins were detected after toxin overlay assays. Following de novo sequencing, a heat-shock cognate protein and a V-ATPase were identified, whilst a approximately 120 kDa protein remained unknown. Additional Cry8Ka5-binding proteins were visualized by two-dimensional gel electrophoresis ligand blots. (c) 2010 Elsevier Inc. All rights reserved.

  10. Vigas de madeira laminada colada com laminas pre-classificadas de eucalyptus grandis

    OpenAIRE

    Grohmann, Sandra Zampieri

    1998-01-01

    Dissertação (Mestrado) - Universidade Federal de Santa Catarina, Centro Tecnologico Comprovar a viabilidade de realização e desempenho de vigas de madeira laminada colada de Eucalyptus grandis classificadas de acordo com sua rigidez cujas lâminas estão divididas em classes de resistência onde o parâmetro de comparação é o módulo de elasticidade longitudinal. Para isso caracterizou-se física e mecanicamente a madeira e fez-se a classificação das laminas em grupos de acordo com o módulo de e...

  11. Magnesium alleviates adverse effects of lead on growth, photosynthesis and ultrastructural alterations of Torreya grandis seedlings

    Directory of Open Access Journals (Sweden)

    Jie Shen

    2016-11-01

    Full Text Available Magnesium (Mg2+ has been shown to reduce the physiological and biochemical stress in plants caused by heavy metals. To date our understanding of how Mg2+ ameliorates the adverse effects of heavy metals in plants is scarce. The potential effect of Mg2+ on lead (Pb2+ toxicity in plants has not yet been studied. This study was designed to clarify the mechanism of Mg2+-induced alleviation of lead (Pb2+ toxicity. Torreya grandis (T. grandis seedlings were grown in substrate contaminated with 0, 700 and 1400 mg Pb2+ per kg-1 and with or without the addition of 1040 mg kg-1 Mg2+. Growth parameters, concentrations of Pb2+ and Mg2+ in the plants’ shoots and roots, photosynthetic pigment, gas exchange parameters, the maximum quantum efficiency (Fv/Fm, root oxidative activity, ultrastructure of chloroplasts and root growth were determined to analyze the effect of different Pb2+ concentrations in the seedlings as well as the potential ameliorating effect of Mg2+ on the Pb2+ induced toxicity. The growth of T. grandis seedlings cultivated in soils treated with 1400 mg kg-1 Pb2+ was significantly reduced compared with that of plants cultivated in soils treated with 0 or 700 mg kg-1 Pb2+. The addition of 1040 mg kg-1 Mg2+ improved the growth of the Pb2+-stressed seedlings, which was accompanied by increased chlorophyll content, the net photosynthetic rate and Fv/Fm, and enhanced chloroplasts development. In addition, the application of Mg2+ induced plants to accumulate five times higher concentrations of Pb2+ in the roots and to absorb and translocate four times higher concentrations of Mg2+ to the shoots than those without Mg2+ application. Furthermore, Mg2+ addition increased root growth and oxidative activity, and protected the root ultrastructure. To the best of our knowledge, our study is the first report on the mechanism of Mg2+-induced alleviation of Pb2+ toxicity. The gener¬ated results may have important implications for understanding the

  12. Annual increments, specific gravity and energy of Eucalyptus grandis by gamma-ray attenuation technique

    International Nuclear Information System (INIS)

    Rezende, M.A.; Guerrini, I.A.; Ferraz, E.S.B.

    1990-01-01

    Specific gravity annual increments in volume, mass and energy of Eucalyptus grandis at thirteen years of age were made taking into account measurements of the calorific value for wood. It was observed that the calorific value for wood decrease slightly, while the specific gravity increase significantly with age. The so-called culmination age for the Annual Volume Increment was determined to be around fourth year of growth while for the Annual Mass and Energy Increment was around the eighty year. These results show that a tree in a particular age may not have a significant growth in volume, yet one is mass and energy. (author)

  13. EFFECT OF ARBUSCULAR MYCORRHIZA FUNGI INOCULATION ON TEAK (Tectona grandis Linn. F AT CIKAMPEK, WEST JAVA

    Directory of Open Access Journals (Sweden)

    Ragil S.B. Irianto

    2005-07-01

    Full Text Available The aim of this study was to identify the effect of Arbuscular Mycorhiza Fungi (AMF on the early growth of teak (Tectona grandis Linn. F plantation. Teak seedlings were inoculated with Glomus aggregatum or Mycofer (mixing of four Arbuscular Mycorrhiza Fungi (AMF : G. margarita, G. manihotis, G. etunicatum and Acalospora spinosa at the time of transplantation. At  three months old the seedlings were planted in Cikampek experimental forest. Results showed that application of G. aggregatum or mycofer to teak could accelerate height and diameter growth by up to 61%and4 7%, respectively, after three months in the field.

  14. Primeiro registro de Oligonychus yothersi (McGregor (Acari: Tetranychidae em Eucalyptus grandis Hill ex Maiden no Brasil First record of Oligonychus yothersi (McGregor (Acari: Tetranychidae on Eucalyptus grandis Hill ex Maiden in Brazil

    Directory of Open Access Journals (Sweden)

    Fabrício Fagundes Pereira

    2005-08-01

    Full Text Available Relata-se a infestação de um ácaro-vermelho em mudas clonais de Eucalyptus grandis Hill ex Maiden, mantidas em casa de vegetação no município de Martinho Campos, Minas Gerais. O ácaro foi observado na parte superior das folhas que exibiam sinais de sucção de seiva e bronzeamento. Essas injúrias causaram desenvolvimento anormal e morte de plantas. O ácaro foi identificado como Oligonychus yothersi (McGregor (Acari: Tetranychidae, e isso representa o primeiro registro dessa espécie em mudas clonais de E. grandis no Brasil.An infestation of the red spider mite was reported in clone seedlings of Eucalyptus grandis Hill ex Maiden under greenhouse conditions, in the municipality of Martinho Campos, Minas Gerais State. The spider mite was found on the leaf upper faces with signs of sap suction and bronzing. Such injuries caused abnormal development and plant death. The spider mite was identified as Oligonychus yothersi (McGregor (Acari: Tetranychidae. This is the first record of O. yothersi on E. grandis seedlings in Brazil.

  15. Molecular cloning of alpha-amylases from cotton boll weevil, Anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance.

    Science.gov (United States)

    Oliveira-Neto, Osmundo B; Batista, João A N; Rigden, Daniel J; Franco, Octávio L; Falcão, Rosana; Fragoso, Rodrigo R; Mello, Luciane V; dos Santos, Roseane C; Grossi-de-Sá, Maria F

    2003-01-01

    Anthonomus grandis, the cotton boll weevil, causes severe cotton crop losses in North and South America. Here we demonstrate the presence of starch in the cotton pollen grains and young ovules that are the main A. grandis food source. We further demonstrate the presence of alpha-amylase activity, an essential enzyme of carbohydrate metabolism for many crop pests, in A. grandis midgut. Two alpha-amylase cDNAs from A. grandis larvae were isolated using RT-PCR followed by 5' and 3' RACE techniques. These encode proteins with predicted molecular masses of 50.8 and 52.7kDa, respectively, which share 58% amino acid identity. Expression of both genes is induced upon feeding and concentrated in the midgut of adult insects. Several alpha-amylase inhibitors from plants were assayed against A. grandis alpha-amylases but, unexpectedly, only the BIII inhibitor from rye kernels proved highly effective, with inhibitors generally active against other insect amylases lacking effect. Structural modeling of Amylag1 and Amylag2 showed that different factors seem to be responsible for the lack of effect of 0.19 and alpha-AI1 inhibitors on A. grandis alpha-amylase activity. This work suggests that genetic engineering of cotton to express alpha-amylase inhibitors may offer a novel route to A. grandis resistance.

  16. Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (Anthonomus grandis).

    Science.gov (United States)

    Franco, Octávio L; Dias, Simoni C; Magalhães, Claudio P; Monteiro, Ana C S; Bloch, Carlos; Melo, Francislete R; Oliveira-Neto, Osmundo B; Monnerat, Rose G; Grossi-de-Sá, Maria Fátima

    2004-01-01

    The cotton boll weevil, Anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the Americas, causing severe losses due to their damage in cotton floral buds. Enzymatic assays using gut extracts from larval and adult boll weevil have demonstrated the presence of digestive serine proteinase-like activities. Furthermore, in vitro assays showed that soybean Kunitz trypsin inhibitor (SKTI) was able to inhibit these enzymes. Previously, in vivo effects of black-eyed pea trypsin chymotrypsin inhibitor (BTCI) have been demonstrated towards the boll weevil pest. Here, when neonate larvae were reared on an artificial diet containing SKTI at three different concentrations, a reduction of larval weight of up to 64% was observed for highest SKTI concentration 500 microM. The presence of SKTI caused an increase in mortality and severe deformities of larvae, pupae and adult insects. This work therefore represents the first observation of a Kunitz trypsin inhibitor active in vivo and in vitro against A. grandis. Bioassays suggested that SKTI could be used as a tool in engineering crop plants, which might exhibit increased resistance against cotton boll weevil.

  17. Use of glass transition temperature for stabilization of board's cracks of Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Fred W. Calonego

    2010-09-01

    Full Text Available The Eucalyptus grandis logs temperatures were determined and correlated with the board's cracks during steaming. Thermocouples were inserted in the logs center, registering their temperatures during steaming at 90"C. The logs were sawed and the board's cracks measured. It was concluded that: (1 the logistic S-shaped curve explains the logs temperature variation; (2 the logs with diameter of 20 to As temperaturas em toras de Eucalyptus grandis, durante a vaporização, foram determinadas e correlacionadas com as rachaduras das tábuas. Nos centros das toras foram inseridos termopares e registradas suas temperaturas durante a vaporização à 90"C. As toras foram desdobradas e as rachaduras das tábuas mensuradas. Concluiu-se que: (1 o modelo estatístico sigmoidal logístico explica a variação da temperatura nas toras; (2 as toras com 20 a <25, 25 a <30 e 30 a <35 cm de diâmetro apresentaram, respectivamente, 84,2"C, 73,1"C e 45,8"C ao final da vaporização; e (3 as rachaduras foramsignificativamente menores nas toras que atingiram a temperatura de transição vítrea.

  18. Determination of chemical elements in Eucalyptus grandis, manured with Ballad's, by neutrons activation analysis

    International Nuclear Information System (INIS)

    Mateus, Natalina de Fatima; Madi Filho, Tufic

    2007-01-01

    The biosolid is a mud resulting from the biological treatment of wasted liquids. It is considered as a profitable alternative and important to minimize the environmental impact generated by the sewage thrown in to sanitary lands, in forest cultures like the Eucalyptus grandis. The objective of this work was to detect which chemical elements are present in Eucalyptus grandis samples, fertilized with different quantities of biosolid. The eucalyptuses of Estacao Experimental de Ciencias Florestais of Itatinga were planted in March of 1998 and collected with five years old. The used biosolid was produced by Station of Treatment of Sewer of Barueri - SP, classified as kind B. For the determination of the presence and quantity of chemical elements in the eucalyptus samples, an analysis technique by neutronic activation (NAA) was used followed by gamma rays spectroscopy. The samples were irradiated in the Nuclear Reactor IEA-R1 of IPEN-SP, followed by the measure of induced gamma rays activity, using a Detector HPGe. The presence, mainly of Br, Mn, Na and K, was detected in all analyzed samples. (author)

  19. Mycorrhizal symbionts of Pisonia grandis and P. sechellarum in Seychelles: identification of mycorrhizal fungi and description of new Tomentella species.

    Science.gov (United States)

    Suvi, Triin; Tedersoo, Leho; Abarenkov, Kessy; Beaver, Katy; Gerlach, Justin; Kõljalg, Urmas

    2010-01-01

    Nyctaginaceae includes species that are predominantly non-mycorrhizal or form arbuscular or ectomycorrhiza. Root-associated fungi were studied from P. grandis and P. sechellarum roots collected respectively on the islands of Cousin and Silhouette in Seychelles. In addition fungal sporocarps were collected from the sampling area. Fungal symbionts were identified from the roots by anatomotyping and rDNA sequencing; sporocarps collected were examined microscopically and sequenced. Three distantly related ectomycorrhizal fungal species belonging to Thelephoraceae were identified from the roots of P. grandis. Sporocarps also were found for two symbionts and described as new Tomentella species. In addition Tomentella species collected from other Seychelles islands were studied and described as new species if there was no close resemblance to previously established species. P. sechellarum was determined to be an arbuscular mycorrhizal plant; three arbuscular mycorrhizal fungal species were detected from the roots. P. grandis is probably associated only with species of Thelephoraceae throughout its area. Only five Tomentella species are known to form ectomycorrhiza with P. grandis and they never have been found to be associated with another host, suggesting adaptation of these fungi to extreme environmental conditions in host's habitat.

  20. Effects of black-eyed pea trypsin/chymotrypsin inhibitor on proteolytic activity and on development of Anthonomus grandis.

    Science.gov (United States)

    Franco, Octávio L; dos Santos, Roseane C; Batista, João A N; Mendes, Ana Cristina M; de Araújo, Marcus Aurélio M; Monnerat, Rose G; Grossi-de-Sá, Maria Fátima; de Freitas, Sonia M

    2003-06-01

    The cotton boll weevil Anthonomus grandis (Boheman) is one of the major pests of cotton (Gossypium hirsutum L.) in tropical and sub-tropical areas of the New World. This feeds on cotton floral fruits and buds causing severe crop losses. Digestion in the boll weevil is facilitated by high levels of serine proteinases, which are responsible for the almost all proteolytic activity. Aiming to reduce the proteolytic activity, the inhibitory effects of black-eyed pea trypsin/chymotrypsin inhibitor (BTCI), towards trypsin and chymotrypsin from bovine pancreas and from midguts of A. grandis larvae and adult insects were analyzed. BTCI, purified from Vigna unguiculata (L.) seeds, was highly active against different trypsin-like proteinases studied and moderately active against the digestive chymotrypsin of adult insects. Nevertheless, no inhibitory activity was observed against chymotrypsin from A. grandis larval guts. To test the BTCI efficiency in vivo, neonate larvae were reared on artificial diet containing BTCI at 10, 50 and 100 microM. A reduction of larval weight of up to approximately 54% at the highest BTCI concentration was observed. At this concentration, the insect mortality was 65%. This work constitutes the first observation of a Bowman-Birk type inhibitor active in vitro and in vivo toward the cotton boll weevil A. grandis. The results of bioassays strongly suggest that BTCI may have potential as a transgene protein for use in engineered crop plants modified for heightened resistance to the cotton boll weevil.

  1. Interspecific hybridization and inbreeding effect in seed from a Eucalyptus grandis x E. urophylla clonal orchard in Brazil

    Directory of Open Access Journals (Sweden)

    Campinhos Eduardo N.

    1998-01-01

    Full Text Available We used allozyme markers to estimate the amount of natural hybridization between Eucalyptus grandis and E. urophylla in a 7.4-hectare commercial hybrid-seed orchard planted in Espírito Santo, Brazil. This orchard was planted in 1982 using a honeycomb design, with each hexagonal plot containing one E. grandis tree surrounded by six E. urophylla trees. There were 267 replicated hexagonal plots in the orchard. Seeds were harvested from the E. grandis clone only. The multilocus outcrossing rate estimated for the E. grandis clone averaged 70.2%, ranging from 33.0 to 99.0% among individual trees. Contaminant pollination, inferred from progeny genotypes containing alleles not present in the seven parental clones, accounted for 14.4% of the hybrid seed. Contaminant pollen was attributed to neighboring eucalyptus stands isolated from the orchard by a 400-m wide belt of native forest. Inbred and hybrid progenies were identified by their allozyme genotypes and transplanted to the field. Field growth of inbred progeny was 30% lower than that of hybrid plants at two and three years of age.

  2. Dietary sugars and proline influence biological parameters of adult Trissolcus grandis, an egg parasitoid of Sunn pest, Eurygaster integriceps

    NARCIS (Netherlands)

    Hajirajabi, Nafiseh; Fazel, Morteza Movahedi; Harvey, Jeffrey A.; Arbab, Abbass; Asgari, Shahriar

    Parasitoids are important natural enemies that are used in the biological control of insect herbivore pests. The egg parasitoid Trissolcus grandis Thompson (Hym. Scelionidae) is a major enemy of the Sunn pest, Eurygaster integriceps Puton (Hem. Scutelleridae), which in turn is one of the most

  3. Brachymeria pandora (Crawford (Hymenoptera, Chalcididae: a new parasitoid of Historis odius (Fabricius (Lepidoptera, Nymphalidae

    Directory of Open Access Journals (Sweden)

    Hélcio R. Gil-Santana

    2005-12-01

    Full Text Available The first record of parasitism of Brachymeria pandora (Crawford, 1914 (Hymenoptera, Chalcididae on Historis odius (Fabricius, 1775 (Lepidoptera, Nymphalidae in the State of Rio de Janeiro, Brazil is presented.Apresenta-se o primeiro registro de parasitismo de Brachymeria pandora (Crawford, 1914 (Hymenoptera, Chalcididae em Historis odius (Fabricius, 1775 (Lepidoptera, Nymphalidae, no estado do Rio de Janeiro, Brasil.

  4. KEANEKARAGAMAN HYMENOPTERA PARASITIKA PADA TIPE EKOSISTEM BERBEDA DI BANGKA TENGAH, KEPULAUAN BANGKA BELITUNG

    Directory of Open Access Journals (Sweden)

    Herry Marta Saputra

    2017-05-01

    Full Text Available Diversity of parasitic Hymenoptera in different ecosystem types in Central Bangka, Bangka-Belitung Islands. Hymenoptera richness is dominated by parasitic species. More than 80% of Hymenoptera play a role as parasitoid on arthropods that are mostly insects. Diversity of parasitic Hymenoptera is widely studied in various types of terrestrial ecosystems including agro-ecosystem and non-agro-ecosystem. This study aimed to invent and compare the diversity of parasitic Hymenoptera in three different ecosystems, i.e., forest, oil palm plantation, and ex-tin mining. The location was located in Central Bangka Regency, Bangka Island. The study was conducted in Juli 2014 until October 2015. Parasitic Hymenoptera was collected with insect sweep net and yellow pan trap on one transect line with 1000 m length. Parasitic Hymenoptera were found on forest as much as 732 morphospecies, 326 morphospecies on oil palm plantations, and 293 morphospecies on ex-tin mining. Diversity and abundance of parasitic Hymenoptera on forest was higher than oil palm plantation and ex-tin mining area. Braconidae family was found dominant on forest, however on oil palm plantation and extin mining area the dominant family was Scelionidae.

  5. Four newly recorded species of the genus Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae) from Korea

    OpenAIRE

    Hye-Rin Lee; Tae-Ho An; Deok-Seo Ku; Bong-Kyu Byun

    2017-01-01

    Four species of the genus Meteorus (Hymenoptera: Braconidae: Euphorinae) are reported for the first time from Korea: Meteorus brevicauda Thomson, 1895, Meteorus colon Haliday, 1835, Meteorus kunashiricus Belokobylskij, 1995, and Meteorus vexator Haliday, 1835. Diagnosis, distribution, and host information are provided for each species. Keywords: Braconidae, Hymenoptera, Korea, Meteorus, New record

  6. Four newly recorded species of the genus Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae from Korea

    Directory of Open Access Journals (Sweden)

    Hye-Rin Lee

    2017-12-01

    Full Text Available Four species of the genus Meteorus (Hymenoptera: Braconidae: Euphorinae are reported for the first time from Korea: Meteorus brevicauda Thomson, 1895, Meteorus colon Haliday, 1835, Meteorus kunashiricus Belokobylskij, 1995, and Meteorus vexator Haliday, 1835. Diagnosis, distribution, and host information are provided for each species. Keywords: Braconidae, Hymenoptera, Korea, Meteorus, New record

  7. Organogênese de explante foliar de clones de Eucalyptus grandis x E. urophylla Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones

    Directory of Open Access Journals (Sweden)

    Elisa Cristina Soares de Carvalho Alves

    2004-05-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos dos reguladores de crescimento TDZ [1-fenil-3-(1,2,3-tia-diazol-5-iluréia], BAP (6-benzilaminopurina e ANA (ácido naftalenoacético no desempenho da propagação in vitro por organogênese de explante foliar de três clones híbridos de Eucalyptus grandis x Eucalyptus urophylla. Houve resposta diferenciada dos clones quanto a intensidade, textura e coloração dos calos, em razão dos tratamentos com os reguladores de crescimento. Os melhores resultados de calejamento dos três genótipos foram observados nos tratamentos com a combinação dos reguladores de crescimento TDZ (0,5 mg L-1 e ANA (0,1 mg L-1, obtendo-se 100% de calejamento no explante foliar. Os piores resultados de calejamento foram observados nos tratamentos com a combinação dos reguladores de crescimento BAP (0,1 mg L-1 e ANA (0,1 mg L-1. Em relação à regeneração, a melhor resposta foi obtida com 1,0 mg L-1 BAP em que 8% dos calos formados a partir de explantes foliares regeneraram gemas, com número médio destas formadas por calo igual a 4,2.The aim of this work was to evaluate the effects of growth regulators TDZ [1-phenil-3-(1,2,3-thiadiazol-5-yl urea], BAP (6-benzilaminopurine e NAA (Naphthalene acetic acid on the in vitro propagation by organogenesis from foliar explants of Eucalyptus grandis x E. urophylla. Depending on the clone used, there were singular responses to growth regulators treatment regarding callusing intensity, texture and color. The best results of the three genotypes used were observed with the TDZ (0.5 mg L-1 and NAA (0.1 mg L-1 treatment, where 100% of the foliar explants presented callus. The worst results were observed with the BAP (0.1 mg L-1 and NAA (0.1 mg L-1 treatment. Subsequently, considering the regeneration process, the best response was achieved with 1.0 mg L-1 BAP, in which 8% of the calli regenerated buds, with an average of 4.2 buds per explant.

  8. Brotación in vitro de yemas de teca (Tectona grandis L. f. Sprouting buds in vitro teak (Tectona grandis L. f.

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    Fabiana Rojas Parajeles

    2012-11-01

    Full Text Available La teca (Tectona grandis L.f. es una especie maderable exótica introducida a Costa Rica para la siembra de plantaciones comercial por la alta demanda de su madera, su rápido crecimiento y su alta calidad. Inicialmente se utilizó la semilla como único material de siembra y la pobre calidad de muchas de las plantaciones impulsó el inicio de programas de mejora genética. La propagación clonal tomó mucha importancia en estos programas y el cultivo in vitro se convirtió en una herramienta valiosa para la propagación masiva de los árboles élites. Por lo anterior, este trabajo se enfocó en evaluar el efecto de varias concentraciones de dos reguladores de crecimiento, bencilaminopurina (BA y ácido indolacético (AIB, solos y en combinación, en la brotación de yemas dormantes y formación de callos de teca. Tanto el análisis estadístico como la observación visual mostraron que el tratamiento que consistió de 0,005 mg/l de AIA fue el mejor para incrementar la brotación de las yemas y para disminuir la formación de callo.Teak (Tectona grandis L. f. is an exotic timber species introduced to Costa Rica for commercial plantations due to the high demand for its wood, rapid growth and high-quality. Initially the seed was use as the only planting material, but the poor quality of many of the plantations resulted in the initiation of genetic improvement programs. The introduction of clonal propagation in these programs and the establishment of in vitro culture techniques became import tools for mass propagation of the selected elite trees. On the foregoing, this work focused on assessing the effect of several concentrations of two growth regulators, benzylaminopurine (BA and indole acetic acid (AIB, alone and in combination, in the budding of dormantes buds and callus formation of teak. Both the statistical analysis and the visual observation showed that the treatment consisted of 0.005 mg/l of IAA was the best to increase the budding of the

  9. Revisión taxonómica de Alytes grandis Brunner (Amphibia, Anura

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    Sanchiz, B.

    1986-12-01

    Full Text Available The holotype and only existing remain referred to Alytes grandis Brunner, 1957, presumably a discoglossid frog from the German Pleistocene, is examined and compared with other living and fossil Palaearctic anurans. The analysis rejects its validity as an independent extinct species, as The material is clearly within the known variability of living Rana temporaria Linnaeus, 1758, of which it should be considered merely a sinonym.

    Se examina el holotipo y único resto atribuido de Alytes grandis Brunner, 1957, un supuesto discoglósido del Pleistoceno alemán, comparándose con otros anuros actuales y fósiles del Paleártico. El análisis permite desechar su pertenencia a una especie extinta independiente, siendo en cambio atribuible con toda claridad a la viviente Rana temporaria Linnaeus, 1758, de la que debe considerarse sinónimo.
    Zum systematischen Status von Alytes grandis Brunner, 1957 (Amphibia, Anura. Der bislang einzig bekannte und von Brunner (1957 beschriebene Rest zu Alytes grandis aus pleistozänen Ablagerungen der Breitenberghöhle bei Gössweinstein (Fränk, Alb wird beschrieben und aufgrund ausführlicher Vergleichsuntersuchungen der rezenten Art Rana temporaria Linnaeus, 1758, zugeordnet, Alytes grandis Brunner, 1957, ist somit als synonyrn zu Rana temporaria zu betrachten.

  10. Migration and dispersal of Anthonomus grandis (Coleoptera: Curculionidae in South America

    Directory of Open Access Journals (Sweden)

    Teodoro STADLER

    2007-01-01

    Full Text Available El presente estudio sobre la dispersión de Anthonomus grandis Boheman, el picudo del algodonero, en Argentina, Brasil, Paraguay y Bolivia, explora las características ecológicas y fisiológicas que han permitido a este insecto dispersarse y establecerse exitosamente en América del Sur. La plasticidad fenotípica de A. grandis se caracteriza por un tiempo de desarrollo flexible, ciclo de vida multivoltino con generaciones superpuestas, la capacidad de alimentarse con polen de diversas familias botánicas así como de otras fuentes de alimento y por su habilidad para migrar y dispersarse con la ayuda del viento. Todo esto hace de esta especie una plaga clave para el cultivo del algodón. Los cultivos de cítricos en Misiones, Argentina, son posibles sitios para la hibernación de esta especie. En esta región fueron capturadas grandes cantidades de individuos prediapausantes, provenientes de algodonales en post-cosecha en Paraguay, atraídos probablemente por compuestos volátiles de cítricos cultivados en la zona. La quiescencia facultativa que atraviesan los adultos ante condiciones adversas, conlleva a un retraso en el desarrollo que se relaciona con las condiciones desfavorables. Esto sugiere que la hibernación en A. grandis puede ser definida como «oligopausa», una forma intermedia de diapausa. Desde su introducción en Brasil en 1983 y hasta el 2006, el picudo se ha dispersado en dirección sudoeste hacia Argentina, a una velocidad promedio de 61 km año-1. Sin embargo, le ha insumido aproximadamente diez años cruzar 250 km, desde Paraguay hacia el centro de la zona algodonera de Argentina. Este progreso más lento se debe probablemente a las acciones llevadas a cabo en el marco del programa de erradicación del picudo del algodonero, por parte del gobierno de Argentina. La llegada del picudo al área central de cultivo de algodón en la Argentina, así como a otras áreas de cultivo en Paraguay y Argentina, confirma el hecho de que

  11. Biomass of clone of Eucalyptus grandis x urophylla for producing briquettes; Biomassa de clone de Eucalyptus grandis x Eucalyptus urophylla para producao de briquetes

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Eder Aparecido; Oguri, Guilherme [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas], e-mail: os_garcias@fca.unesp.br; Lancas, Kleber Pereira [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Engenharia Rural; Guerra, Saulo Philipe Sebastiao [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Gestao e Tecnologia Agroindustrial

    2011-07-01

    The aim of this work was conducted to address forest biomass energy for briquette producing. In an area of dystrophic soil, seedlings of clones of Eucalyptus grandis x E. urophylla were planted in 2008, considering factors spacing and fertilization. The first dosage of fertilizer was 70 g/plant of NPK 6-30-6 and total coverage of 110 g/plant of NPK 20-0-20 with B and Zn. The spacing was 2.8x0.5 m, 2.8x1.0 m, 2.8x1.5 m, 2.8x2.0 m and 2.8x2.5 m. At 18 months, tree samples were collected to evaluate the basic density of wood (BDW), dry biomass of stem, branches and leaves. An assessment of the economic viability of each treatment was based on the sale of briquettes. BDW spacing of 2.8x1.0 m was 0.464 kg/m{sup 3}. The largest biomass of the stem occurred in 2.8x0.5 m spacing, with dosage 3, but economically unviable. The dry biomass of branches was only affected by dosage, reaching 17.68 t/ha in the third dose. Only fertilization was significant for leaf biomass. The highest income in the spacing was 2.8 x1.5 m with dosage 2. (author)

  12. Allergen-specific immunotherapy of Hymenoptera venom allergy

    DEFF Research Database (Denmark)

    Schiener, Maximilian; Graessel, Anke; Ollert, Markus

    2017-01-01

    Stings of hymenoptera can induce IgE-mediated hypersensitivity reactions in venom-allergic patients, ranging from local up to severe systemic reactions and even fatal anaphylaxis. Allergic patients' quality of life can be mainly improved by altering their immune response to tolerate the venoms...... by injecting increasing venom doses over years. This venom-specific immunotherapy is highly effective and well tolerated. However, component-resolved information about the venoms has increased in the last years. This knowledge is not only able to improve diagnostics as basis for an accurate therapy......, but was additionally used to create tools which enable the analysis of therapeutic venom extracts on a molecular level. Therefore, during the last decade the detailed knowledge of the allergen composition of hymenoptera venoms has substantially improved diagnosis and therapy of venom allergy. This review focuses...

  13. Site of pheromone biosynthesis and isolation of HMG-CoA reductase cDNA in the cotton boll weevil, Anthonomus grandis.

    Science.gov (United States)

    Taban, A Huma; Fu, Jessica; Blake, Jacob; Awano, Ami; Tittiger, Claus; Blomquist, Gary J

    2006-08-01

    Isolated gut tissue from male cotton boll weevil, Anthonomus grandis (Coleoptera: Curculionidae), incorporated radiolabeled acetate into components that co-eluted with monoterpenoid pheromone components on HPLC. This demonstrates that pheromone components of male A. grandis are produced de novo and strongly suggests that pheromone biosynthesis occurs in gut tissue. A central enzyme in isoprenoid biosynthesis is 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R), and a full-length HMG-R cDNA was isolated from A. grandis. The predicted translation product was 54 and 45% identical to HMG-R from Ips paraconfusus and Drosophila melanogaster, respectively. HMG-R gene expression gradually increased with age in male A. grandis, which correlates with pheromone production. However, topical application of JH III did not significantly increase HMG-R mRNA levels.

  14. Fractionation of organic substances from the South African Eucalyptus grandis biomass by a combination of hot water and mild alkaline treatments

    CSIR Research Space (South Africa)

    Johakimu, Jonas K

    2015-09-01

    Full Text Available An alternative way of fractionating lignocellulose biomass into its individual components, hemicelluloses, lignin and cellulose, was investigated. South African Eucalyptus grandis wood chips were fractionated using a combination of hot water...

  15. História natural da interação entre Meteorus sp. Haliday, 1835 (Hymenoptera: Braconidae) e seu hiperparasitoide Toxeumella albipes Girault, 1913 (Hymenoptera: Pteromalidae)

    OpenAIRE

    Sobczak, JF; Maia, DP; Moura, JCMS; Costa, VA; Vasconcellos-Neto, J

    2012-01-01

    Some parasitoids build a cocoon mass that hangs in the host body until the adults emergence, which is an advantage against attack by predators who troll the vegetation in search of prey. However, such behaviour is not effective against the hyperparasitoid attacks. This study reports the interaction between the caterpillar Manduca sexta Linnaeus, 1763 (Lepidoptera, Sphingidae) parasitised by Meteorus sp. (Hymenoptera, Braconidae) larvae and its hyperparasitoid Toxeumella albipes (Hymenoptera, ...

  16. On the identity of Melipona torrida Friese (Hymenoptera, Apidae)

    OpenAIRE

    Melo, Gabriel A. R.

    2013-01-01

    On the identity of Melipona torrida Friese (Hymenoptera, Apidae). Melipona marginata var. torrida Friese, 1916, described from three workers putatively collected in Costa Rica, never had its identity properly recognized. Since its original description, no additional specimens have ever been collected in Costa Rica. It is argued here that Melipona torrida was based on mislabeled specimens and corresponds to Melipona marginata obscurior Moure, 1971, a form known only from southern Brazil, Argen...

  17. Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)

    Czech Academy of Sciences Publication Activity Database

    Carabajal Paladino, Leonela Z.; Lo Nostro, F.; Papeschi, A.; Cladera, J.; Bressa, M. J.

    2017-01-01

    Roč. 98, č. 1 (2017), s. 38-43 ISSN 0001-7272 R&D Projects: GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : Hymenoptera * modified meiosis * abortive division Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Genetics and heredity (medical genetics to be 3) Impact factor: 1.211, year: 2016 http://onlinelibrary.wiley.com/doi/10.1111/azo.12148/pdf

  18. Salicylic acid alleviates the adverse effects of salt stress in Torreya grandis cv. Merrillii seedlings by activating photosynthesis and enhancing antioxidant systems.

    Directory of Open Access Journals (Sweden)

    Tingting Li

    Full Text Available BACKGROUND: Salt stress is a major factor limiting plant growth and productivity. Salicylic acid (SA has been shown to ameliorate the adverse effects of environmental stress on plants. To investigate the protective role of SA in ameliorating salt stress on Torreya grandis (T. grandis trees, a pot experiment was conducted to analyze the biomass, relative water content (RWC, chlorophyll content, net photosynthesis (Pn, gas exchange parameters, relative leakage conductivity (REC, malondialdehyde (MDA content, and activities of superoxide dismutase (SOD and peroxidase (POD of T. grandis under 0.2% and 0.4% NaCl conditions with and without SA. METHODOLOGY/PRINCIPAL FINDINGS: The exposure of T. grandis seedlings to salt conditions resulted in reduced growth rates, which were associated with decreases in RWC and Pn and increases in REC and MDA content. The foliar application of SA effectively increased the chlorophyll (chl (a+b content, RWC, net CO2 assimilation rates (Pn, and proline content, enhanced the activities of SOD, CAT and POD, and minimized the increases in the REC and MDA content. These changes increased the capacity of T. grandis in acclimating to salt stress and thus increased the shoot and root dry matter. However, when the plants were under 0% and 0.2% NaCl stress, the dry mass of the shoots and roots did not differ significantly between SA-treated plants and control plants. CONCLUSIONS: SA induced the salt tolerance and increased the biomass of T. grandis cv. by enhancing the chlorophyll content and activity of antioxidative enzymes, activating the photosynthetic process, and alleviating membrane injury. A better understanding about the effect of salt stress in T. grandis is vital, in order gain knowledge over expanding the plantations to various regions and also for the recovery of T. grandis species in the future.

  19. Cross-reacting carbohydrate determinants and hymenoptera venom allergy.

    Science.gov (United States)

    Brehler, Randolf; Grundmann, Sonja; Stöcker, Benedikt

    2013-08-01

    Insect venom allergy is an important cause of anaphylaxis. Venom immunotherapy assume the clear identification of the culprit insect, but this is impeded by Immunoglobulin E (IgE) antibodies to cross reactive carbohydrate determinant (CCD) epitopes of common glycoproteins. Here we give an overview about inducers, importance, and relevance of anti-N-Glycan CCD IgE antibodies. Pollen exposure and insect stings induce anti-CCD IgE antibodies interfering with in-vitro tests for allergy diagnosis due to extensive IgE cross-reactivity. Instead of being biologically active these antibodies are irrelevant for allergic reactions due to hymenoptera stings. The general response of the immune system to the ubiquitous exposure to N-glycan containing glycoproteins is still a matter of debate. CCD specific IgG antibodies in sera of bee keepers suggest tolerance induction due to high-dose exposure. Tolerance induction by pollen and food glycoproteins has not been proved. Hymenoptera stings and pollen exposure induce anti-CCD IgE. In regard to anaphylaxis due to Hymenoptera stings these antibodies are not clinically relevant, but they are important for the specificity of in-vitro tests proving insect venom allergy. The introduction of component based diagnostic IgE testing improves the specificity of in-vitro tests if proteins devoid of CCD epitopes are used.

  20. Modification of the original color of the Eucalyptus grandis wood by heat treatments

    Directory of Open Access Journals (Sweden)

    Rosilei Aparecida Garcia

    2014-09-01

    Full Text Available The objective of this study was to determine the modification of original color of Eucalyptus grandis Hill ex. Maiden wood after heat-treatment. Wood samples were heat-treated under different temperatures (180, 200, 215 and 230ºC and time conditions (15 minutes, 2 and 4 hours. Color analysis were performed on the CIE L*a*b* system by using a Color Eye XTH-X-Rite 200d spectrophotometer. All heat treatments promoted an alteration of the original color of wood. Heat-treated woods presented lower L* (lightness values than untreated wood (control, characterizing the wood darkness, mainly for more severe conditions of temperature and time. Chromatic coordinates (a* and b* showed different behaviors depending on the temperature-time combination. The modification of the original color of the wood allowed the creation of new color patterns, which can add greater value to the studied wood.

  1. EFFECT OF THE STEAMING ON THE DRYING OF Eucalyptus grandis BOARDS

    Directory of Open Access Journals (Sweden)

    Raphael Nogueira Rezende

    2015-03-01

    Full Text Available The objective of this work was to evaluate the effect of the steaming on the drying rate and drying quality of Eucalyptus grandis boards. For this purpose, wood from an experimental plantation of the Federal University of Lavras, Minas Gerais State, Brazil, with 24 years of age was used. Trees were felled and sectioned in logs and the logs were sawn by a tangential system. Half of the boards volume were steamed during 3 hours at 90ºC of temperature and 100% of relative humidity after the heating of the drying process. The other half was not steamed (control. The boards were dried in the dry-kiln and the resulting defects from the drying process and drying rate were determined. The results indicated that the steaming was effective in increase of the drying rate in 15% and decrease of the drying defects of 20 to 52%.

  2. Development Potential, in a Greenhouse, of Cuttings of the Hybrid Eucalyptus grandis x Eucalyptus camaldulensis

    Directory of Open Access Journals (Sweden)

    M. N. Garcia

    2013-03-01

    Full Text Available Abstract: This study evaluated the potential for development in the greenhouse, cuttings of the hybrid of Eucalyptus grandis x Eucalyptus camaldulensis in Sinop - Mato Grosso and generated equations to estimate the percentage of rooting parameters. Were measured: the percentage of cuttings with some degree of modification, the percentage of cuttings in the percentage of cuttings with roots larger than 10 cm in length. Also measured was the neck diameter, height and leaf area index. The heights and leaf area showed lower development within the greenhouse. The rooting potential is based on curve speed of rooting and was found 12 days after staking. The identification of the optimal point of withdrawal of the shoots of the greenhouse through the intercept curves daily current increase curve (DCI e daily average increase (DAI, being 19 days after staking. The company derives the stakes with 24 days underdoing the potential of the species and structures used.Keywords: Eucalyptus, greenhouse management and rooting

  3. JH III production, titers and degradation in relation to reproduction in male and female Anthonomus grandis.

    Science.gov (United States)

    Taub-Montemayor, Tina E; Min, Kyung-Jin; Chen, Zhaorigetu; Bartlett, Terri; Rankin, Mary Ann

    2005-04-01

    Juvenile hormone (JH) is necessary for the production of vitellogenin (Vg) in the boll weevil, Anthonomus grandis. Occurrence of Vg in this species is typically restricted to reproductively competent females, and is not detected in untreated males. However, the JH analog, methoprene stimulates Vg production in intact males and in the isolated abdomens of both male and female boll weevils (where in each case no Vg is detected without treatment), suggesting that males are competent to produce Vg but are normally not stimulated to do so. Preliminary work indicating that male boll weevil corpora allata (CA) produced little or no JH in vitro suggested that failure of males to produce Vg might be due to very low JH levels compared to females. This study re-examines the question of JH in male boll weevils by determining in vitro production of JH III by male CA during the first 10 days after adult emergence, determining hemolymph JH esterase activity during this same time period and hemolymph JH III titers in adults of both sexes. We also re-examine the ability of isolated male abdomens to produce Vg in response to hormonal stimulation, analyzing the effect of a wide range of methoprene and JH III dosages. Results indicate that male A. grandis have circulating JH titers and JH production similar to females. JH esterase activity is slightly but significantly higher in males than females. Vg production by isolated abdomens of both sexes after stimulation with methoprene or JH III was confirmed. Dose response studies indicated that high levels of methoprene were less effective than intermediate doses in stimulating Vg synthesis in both sexes. We conclude that the sexually dimorphic effect of JH on Vg synthesis is not due to differences in JH production or differences in JH titer between the sexes.

  4. Selection of Bacillus thuringiensis strains toxic to cotton boll weevil (Anthonomus grandis, Coleoptera: Curculionidae) larvae.

    Science.gov (United States)

    Pérez, Melisa P; Sauka, Diego H; Onco, María I; Berretta, Marcelo F; Benintende, Graciela B

    Preliminary bioassays with whole cultures (WC) of 124 Bacillus thuringiensis strains were performed with neonate larvae of Anthonomus grandis, a major cotton pest in Argentina and other regions of the Americas. Three exotic and four native strains were selected for causing more than 50% mortality. All of them were β-exotoxin producers. The native strains shared similar morphology of parasporal crystals, similar protein pattern and identical insecticidal gene profiles. These features resembled Lepidoptera-toxic strains. Furthermore, these strains showed a Rep-PCR pattern identical to lepidoptericidal strain HD-1, suggesting that these strains may belong to serovar kurstaki. However, some differences were observed in the plasmid profiles and in the production of β-exotoxin. To determine the culture fractions where the insecticidal metabolites were present, bioassays including resuspended spore-crystal pellets, filtered supernatants (FS) were compared with those of WC. Both fractions tested showed some level of insecticidal activity. The results may suggest that the main toxic factors can be found in FS and could be directly correlated with the presence of β-exotoxin. Based on the bioassays with FS and autoclaved FS, the participation of thermolabile virulence factors such as Cry1I in toxicity is neither discarded. In the selected strains, β-exotoxin would be the major associated virulence factor; therefore, their use in biological control of A. grandis should be restricted. Nevertheless, these strains could be the source of genes (e.g., cry1Ia) to produce transgenic cotton plants resistant to this pest. Copyright © 2017 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  5. Antioxidant capacity and larvicidal activity of essential oil and extracts from Lippia grandis

    Directory of Open Access Journals (Sweden)

    Evelyn Ivana T. Damasceno

    2011-02-01

    Full Text Available The leaves and thin branches of Lippia grandis Schauer, Verbenaceae, are used for flavoring of food in the Brazilian Amazon, as substitute for oregano. In this study the constituents of the essential oil were identified and the antioxidant capacity and larvicidal activity of the oil and methanol extract and its sub-fractions were evaluated. A sensory evaluation was determined in view of absence of toxicity. The oil showed a yield of 2.1% and its main constituents were thymol (45.8%, p-cymene (14.3%, γ-terpinene (10.5%, carvacrol (9.9% and thymol methyl ether (4.8%, totalizing 85%. The DPPH radical scavenging activity showed values for the EC50 between 9.0 and 130.5 µg mL-1 and the TEAC/ABTS values varied from 131.1 to 336.0 mg TE/g, indicating significant antioxidant activity for the plant. The total phenolic content ranged from 223.0 to 761.4 mg GAE/g, contributing to the antioxidant activity observed. The crude extracts inhibited the bleaching of β-carotene and the oil showed the greatest inhibition (42.5%. The oil (LgO, 7.6±2.4 µg mL-1 showed strong larvicidal activity against the brine shrimp bioassay. The sensory evaluation was highly satisfactory in comparison to oregano. The results are very promising for the use of L. grandis in seasoning and antioxidant products.

  6. Temperature effects on wood anatomy, wood density, photosynthesis and biomass partitioning of Eucalyptus grandis seedlings.

    Science.gov (United States)

    Thomas, D S; Montagu, K D; Conroy, J P

    2007-02-01

    Wood density, a gross measure of wood mass relative to wood volume, is important in our understanding of stem volume growth, carbon sequestration and leaf water supply. Disproportionate changes in the ratio of wood mass to volume may occur at the level of the whole stem or the individual cell. In general, there is a positive relationship between temperature and wood density of eucalypts, although this relationship has broken down in recent years with wood density decreasing as global temperatures have risen. To determine the anatomical causes of the effects of temperature on wood density, Eucalyptus grandis W. Hill ex Maiden seedlings were grown in controlled-environment cabinets at constant temperatures from 10 to 35 degrees C. The 20% increase in wood density of E. grandis seedlings grown at the higher temperatures was variously related to a 40% reduction in lumen area of xylem vessels, a 10% reduction in the lumen area of fiber cells and a 10% increase in fiber cell wall thickness. The changes in cell wall characteristics could be considered analogous to changes in carbon supply. Lumen area of fiber cells declined because of reduced fiber cell expansion and increased fiber cell wall thickening. Fiber cell wall thickness was positively related to canopy CO2 assimilation rate (Ac), which increased 26-fold because of a 24-fold increase in leaf area and a doubling in leaf CO2 assimilation rate from minima at 10 and 35 degrees C to maxima at 25 and 30 degrees C. Increased Ac increased seedling volume, biomass and wood density; but increased wood density was also related to a shift in partitioning of seedling biomass from roots to stems as temperature increased.

  7. The gall wasp Leptocybe invasa (Hymenoptera: Eulophidae) stimulates different chemical and phytohormone responses in two Eucalyptus varieties that vary in susceptibility to galling.

    Science.gov (United States)

    Li, X Q; Liu, Y Z; Guo, W F; Solanki, M K; Yang, Z D; Xiang, Y; Ma, Z C; Wen, Y G

    2017-09-01

    Gall-inducing insects produce various types of galls on plants, but little is known about the gall-induction mechanism of these galling insects. The gall wasp Leptocybe invasa Fisher & LaSalle (Hymenoptera: Eulophidae) forms galls of different sizes on several Eucalyptus species. To clarify the physiological responses of Eucalyptus to L. invasa infestation, we measured the dynamics of nitrogen (N), carbon (C), total phenolics, total tannins and four types of phytohormones (zeatin [Z] + zeatin riboside [ZR], gibberellins [GA], indole-3-acetic acid [IAA] and abscisic acid [ABA]) in galled and ungalled leaf tissues of two Eucalyptus horticultural varieties (DH201-2 [Eucalyptus grandis × Eucalyptus camaldulensis] and EA [Eucalyptus exserta]) with different susceptibility to galling throughout the larval developmental stages. Nitrogen, total phenolics, tannins and four kinds of phytohormones strongly accumulated in tissues galled by L. invasa (especially during early larval feeding stages). While N, Z + ZR and GA levels were higher, tannins and ABA levels were lower in the galled tissues on the highly susceptible variety. Nitrogen, total phenolics, GA, Z + ZR and IAA levels in the galled tissues gradually decreased during gall development, but ABA and tannins conversely increased in the galled tissues of the less susceptible variety. Our results suggest that the effects of gall-inducing insects on plants depend not only on the susceptibility of the plant infested but also on the developmental stage of galled tissues. Gall formation process is thus synergistically influenced by both gall-inducing insect and plant genotypes. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  8. Simulated drift effect of glyphosate in different parts of Eucalyptus grandis plantsEfeito da deriva simulada de glyphosate em diferentes partes da planta de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Maria Renata Rocha Pereira

    2012-10-01

    Full Text Available The present work aimed to evaluate the effects of simulated drift of glyphoste on Eucalyptus grandis, through the application of low doses in different parts of the plant. The experimental design was a randomized block design with five replications. The treatments were glyphosate application at 0; 30; 60; 90 e 120 g a.e. ha-1 of the commercial formulation Scout®. Three forms of application were used: applying on leaf, on stem, and on the entire plant (leaf + stem. For leaf application, stems were covered with plastic ribbons to protect them from the solution; the same was made with plants that were sprayed on stems, covering leaf with plastic bag. The application was carried out in an armed stationary spray tips XR 11002 VS, with 183 KPa pressure in volume of 200 L ha-1. The eucalyptus plants receiving applications in leaves and whole plant (leaves + stem showing effects of intoxication are more intense about the plants that received the stem applications only. However, there may be increases in height growth and total dry mass of eucalyptus plants in applications of 30 g a.e. ha-1 glyphosate.No presente trabalho objetivou-se avaliar os efeitos da deriva simulada de glyphoste em plantas de Eucalyptus grandis, por meio da aplicação de doses reduzidas em diferentes partes da planta. Utilizouse o delineamento inteiramente casualizado, com cinco repetições. Os tratamentos foram constituídos da aplicação de 0; 30; 60; 90 e 120 g e.a.ha-1 de glyphosate, da formulação comercial Scout®. A aplicação foi realizada de três formas: aplicação sobre as folhas, no caule e na planta inteira (folha + caule. Para a aplicação nas folhas o caule foi coberto com fitas plásticas para evitar que fosse atingido pela solução, e o mesmo foi feito com as plantas que receberam pulverização no caule, cobrindo as folhas com saco plástico. A aplicação foi realizada em um pulverizador estacionário, munido de pontas XR 11002 VS, com pressão de 183

  9. Abridged life tables for Cephalonomia stephanoderis and Prorops nasuta (Hymenoptera: Bethylidae) Parasitoids of Hypothenemus hampei (Coleoptera: Scolytidae) reared on artificial diet

    Science.gov (United States)

    Biological aspects and demographic parameters of Cephalonomia stephanoderis Betrem (Hymenoptera: Bethylidae) and Prorops nasuta Waterston (Hymenoptera: Bethylidae) parasitoids of the coffee berry borer (CBB), Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) were investigated usi...

  10. Activity of a Brazilian strain of Bacillus thuringiensis israelensis against the cotton Boll Weevil Anthonomus grandis Boheman (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Monnerat, R; Martins, E; Praça, L; Dumas, V; Berry, C

    2012-02-01

    A Brazilian Bacillus thuringiensis subspecies israelensis, toxic to Diptera, including mosquitoes, was found also to show toxicity to the coleopteran boll weevil Anthonomus grandis Boheman at an equivalent level to that of the standard coleopteran-active B. thuringiensis subspecies tenebrionis T08017. Recombinant B. thuringiensis strains expressing the individual Cyt1Aa, Cry4Aa, Cry4Ba and Cry11Aa toxins from this strain were assessed to evaluate their potential contribution to the activity against A. grandis, either alone or in combination. Whilst individual toxins produced mortality, none was sufficiently potent to allow calculation of LC50 values. Combinations of toxins were unable to attain the same potency as the parental B. thuringiensis subsp. israelensis, suggesting a major role for other factors produced by this strain.

  11. Antraquinonas e naftoquinonas do caule de um espécime de reflorestamento de Tectona grandi (Verbenaceae

    Directory of Open Access Journals (Sweden)

    Rafael Y.O. Moreira

    Full Text Available O fracionamento do extrato hexânico do caule de um espécime de reflorestamento de Tectona grandis (Verbenaceae, através de procedimentos fitoquímicos clássicos, levou ao isolamento das naftoquinonas lapachol e desidro-a-lapachona e das antraquinonas tectoquinona e obtusifolina. As estruturas das substâncias foram caracterizadas através da análise de métodos espectrométricos de RMN. Este é o primeiro estudo fitoquímico de um espécime de reflorestamento de Tectona grandis, no Brasil, sendo o objetivo principal deste trabalho a comprovação da presença de tectoquinona em espécimes cultivados.

  12. Antioxidant, antiglycation and insulinotrophic properties of Coccinia grandis (L. in vitro: Possible role in prevention of diabetic complications

    Directory of Open Access Journals (Sweden)

    Packirisamy Meenatchi

    2017-01-01

    Full Text Available In an attempt to develop Complementary and Alternative Medicine (CAM for the treatment of diabetes and related complications, the antidiabetic potential of the mature unripe fruits of Coccinia grandis (CGF was evaluated. Oxidative stress and glycation plays an important role in manifesting of diabetes and vascular complications. Agents with antioxidant and antiglycation properties may retard these pathological alterations. In this study, the edible plant Coccinia grandis was assessed for in vitro estimation of antioxidant and antiglycation potential and its insulinotrophic properties in RINm5F cells. Antioxidant activity was evaluated as DPPH (1,1-diphenyl-2-picrylhydrazyl, hydrogen peroxide and superoxide anion scavenging activities, whereas the protein glycation inhibitory potential was evaluated using in vitro albumin-fructose glycation model. Glycation inhibition was estimated by different biochemical parameters viz. fructosamine, protein carbonyl group and protein aggregation using thioflavin T fluorescence. C. grandis extract exerted a dose dependent radical scavenging activity and exhibited a significant antiglycation potential. The extract also showed a significant insulinotrophic property with 1.28 and 1.71-fold increase in insulin release when compared to control at 0.25 and 0.50 mg/mL, respectively. These data suggest the possible antidiabetic role of CGF extract, presumably by its antioxidant, antiglycation and insulin secretory effects. Present findings provide experimental evidence that the fruits of C. grandis have potential antidiabetic activity which might be used as a functional food and safe remedy for the treatment of diabetes and associated complications. This study also revealed that the plant can be a promising source for development of natural antiglycating agents and novel insulin secretagogues.

  13. René Descartes, the Robots and the Soul in the debate between Tommaso Ceva and Guido Grandi

    Directory of Open Access Journals (Sweden)

    Alfredo Serrai

    2016-05-01

    Full Text Available Descartes outlined human intelligence as a divine gift, albeit in a system governed by universal laws of physics and mathematics. In the eighteenth century the camaldolite Guido Grandi and Tommaso Ceva, both mathematicians, had a dispute about the interpretation of the Cartesian model of human intelligence. This case provides an opportunity to retrace the path of a speculative techno-philosophical question concerning the human possibility to equipping robots with the ability to experience a self-consciousness.

  14. Dynamic transcriptome profiling of the floral buds in the dioecious cucurbit Coccinia grandis using RNA-Seq

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    Jatindra Nath Mohanty

    2017-10-01

    Full Text Available Angiosperms exhibits diversified sexual systems encompassing bisexual, monoecious and dioecious conditions. Dioecy offers opportunities to explore separately, the male and female systems giving an insight into the evolutionary, developmental and molecular processes of sex expression in plants. Coccinia grandis (Family: Cucurbitaceae with small genome size and heteromorphic sex chromosomes is often considered a model dioecious plant for sex evolution. However, the information relating to its genetic orientation, physical state and sex determining factors is highly ambiguous and limited. In the present study we have attempted to identify the molecular basis of sex determination in C. grandis through genome wide transcriptome profiling of the floral buds. About 75 million clean reads were generated resulting in 72,479 unigenes for male library and 63,308 unigenes for female library with a mean length of 736 bp. 1410 unigenes were differentially expressed (DEGs between the male and female buds as identified from the RNA-Seq pattern and qRT-PCR validation. Functional annotation using BLAST2GO and KEGG revealed high enrichment of DEGs in phytohormone biosynthesis, hormone signaling and transduction, transcriptional regulation and methyl transferase activity. Manifold up-regulation of genes phytohormone responsive genes such as ARF6, ACC synthase1, SNRK2 and BRI1-associated receptor kinase 1 (BAK1 suggest that a signaling crosstalk is implicated in the sex determination of this species. Besides, a wide range of transcription factors including zinc fingers, homeodomain leucine zippers and MYBs were recognized as major determinants of male specific expression in the dioecious plant. Additionally, C. grandis transcriptome revealed 48 target genes for many miRNAs sequences with established role in floral development and sex determination. Overall, our study resulted in the identification of a large amount of molecular resources that could be critical to

  15. Molecular cloning of a cysteine proteinase cDNA from the cotton boll weevil Anthonomus grandis (Coleoptera: Curculionidae).

    Science.gov (United States)

    De Oliveira Neto, Osmundo Brilhante; Batista, João Aguiar Nogueira; Rigden, Daniel John; Franco, Octávio Luiz; Fragoso, Rodrigo Rocha; Monteiro, Ana Carolina Santos; Monnerat, Rose Gomes; Grossi-De-Sa, Maria Fátima

    2004-06-01

    The cotton boll weevil (Anthonomus grandis) causes severe cotton crop losses in North and South America. This report describes the presence of cysteine proteinase activity in the cotton boll weevil. Cysteine proteinase inhibitors from different sources were assayed against total A. grandis proteinases but, unexpectedly, no inhibitor tested was particularly effective. In order to screen for active inhibitors against the boll weevil, a cysteine proteinase cDNA (Agcys1) was isolated from A. grandis larvae using degenerate primers and rapid amplification of cDNA ends (RACE) techniques. Sequence analysis showed significant homologies with other insect cysteine proteinases. Northern blot analysis indicated that the mRNA encoding the proteinase was transcribed mainly in the gut of larvae. No mRNA was detected in neonatal larvae, pupae, or in the gut of the adult insect, suggesting that Agcys1 is an important cysteine proteinase for larvae digestion. The isolated gene will facilitate the search for highly active inhibitors towards boll weevil larvae that may provide a new opportunity to control this important insect pest.

  16. Identification of PEG-induced water stress responsive transcripts using co-expression network in Eucalyptus grandis.

    Science.gov (United States)

    Ghosh Dasgupta, Modhumita; Dharanishanthi, Veeramuthu

    2017-09-05

    Ecophysiological studies in Eucalyptus have shown that water is the principal factor limiting stem growth. Effect of water deficit conditions on physiological and biochemical parameters has been extensively reported in Eucalyptus. The present study was conducted to identify major polyethylene glycol induced water stress responsive transcripts in Eucalyptus grandis using gene co-expression network. A customized array representing 3359 water stress responsive genes was designed to document their expression in leaves of E. grandis cuttings subjected to -0.225MPa of PEG treatment. The differentially expressed transcripts were documented and significantly co-expressed transcripts were used for construction of network. The co-expression network was constructed with 915 nodes and 3454 edges with degree ranging from 2 to 45. Ninety four GO categories and 117 functional pathways were identified in the network. MCODE analysis generated 27 modules and module 6 with 479 nodes and 1005 edges was identified as the biologically relevant network. The major water responsive transcripts represented in the module included dehydrin, osmotin, LEA protein, expansin, arabinogalactans, heat shock proteins, major facilitator proteins, ARM repeat proteins, raffinose synthase, tonoplast intrinsic protein and transcription factors like DREB2A, ARF9, AGL24, UNE12, WLIM1 and MYB66, MYB70, MYB 55, MYB 16 and MYB 103. The coordinated analysis of gene expression patterns and coexpression networks developed in this study identified an array of transcripts that may regulate PEG induced water stress responses in E. grandis. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Impacto das capinas mecânica e química do sub-bosque de Eucalyptus grandis sobre a comunidade de formigas (Hymenoptera: Formicidae Impact of mechanical and chemical weedings of Eucalyptus grandis undergrowth on an ant community (Hymenoptera: Formicidae

    Directory of Open Access Journals (Sweden)

    Lucimeire de Souza Ramos

    2004-02-01

    Full Text Available O efeito das capinas mecânica e química do sub-bosque em plantações de eucaliptos e as conseqüências desses tratamentos sobre a comunidade de formigas foram avaliados no município de Bom Despacho, Minas Gerais, Brasil. As formigas foram coletadas com o extrator de Winkler. Coletou-se um total de 86 espécies, pertencentes a seis subfamílias. Oito dias após as capinas, o número de espécies reduziu-se de um quarto para os dois tipos de capina. Sessenta dias após, o número de espécies tendeu a retornar ao estágio inicial, verificando-se que a eliminação do sub-bosque causa efeito deletério imediato, de igual intensidade e de pouca duração sobre a comunidade de formigas. As razões das variações observadas serão discutidas.The effect of mechanical and chemical undergrowth weedings on an ant community was tested in eucalypts plantations and the consequences of such treatments were evaluated at Bom Despacho, Minas Gerais State, Brazil. The ants were collected by applying the Winkler trap method. Eighty-six species of six sub-families were found. Eight days after the clearings, the number of species dropped to 1/4 in both weedings systems. Sixty days later, the number of species tended to return to the initial level, showing that undergrowth elimination caused an immediate depressive effect on the ant community of similar intensity and over a short time. The reasons of the variations observed are discussed.

  18. A NEW SPECIES OF INVASIVE GALL WASP (HYMENOPTERA: EULOPHIDAE: TETRASTICHINAE) ON BLUE GUM (EUCALYPTUS GLOBULUS) IN CALIFORNIA

    Science.gov (United States)

    The blue gum gall wasp, Selitrichodes globulus La Salle & Gates (Hymenoptera: Eulophidae: Tetrastichinae), is described as an invasive gall inducer on blue gum, Eucalyptus globulus (Myrtaceae), in California....

  19. A new species of Chelonus (Areselonus) (Hymenoptera, Braconidae) from India reared from Acrocercops lysibathra (Meyrick) on Cordia latifolia Roxb.

    OpenAIRE

    Ahmad, Zubair; Ghramh, Hamed A.

    2018-01-01

    Chelonus (Areselonus) spinigaster sp. n., (Hymenoptera: Braconidae: Cheloninae) is described from India. The new species was reared from the moth species Acrocercops lysibathra (Meyr.) on Cordia latifolia Roxb.

  20. A new species of Chelonus (Areselonus) (Hymenoptera, Braconidae) from India reared from Acrocercops lysibathra (Meyrick) on Cordia latifolia Roxb.

    Science.gov (United States)

    Ahmad, Zubair; Ghramh, Hamed A

    2018-01-01

    Chelonus (Areselonus) spinigaster sp. n. , (Hymenoptera: Braconidae: Cheloninae) is described from India. The new species was reared from the moth species Acrocercops lysibathra (Meyr.) on Cordia latifolia Roxb.

  1. Large-scale transcriptional profiling of lignified tissues in Tectona grandis.

    Science.gov (United States)

    Galeano, Esteban; Vasconcelos, Tarcísio Sales; Vidal, Mabel; Mejia-Guerra, Maria Katherine; Carrer, Helaine

    2015-09-15

    Currently, Tectona grandis is one of the most valuable trees in the world and no transcript dataset related to secondary xylem is available. Considering how important the secondary xylem and sapwood transition from young to mature trees is, little is known about the expression differences between those successional processes and which transcription factors could regulate lignin biosynthesis in this tropical tree. Although MYB transcription factors are one of the largest superfamilies in plants related to secondary metabolism, it has not yet been characterized in teak. These results will open new perspectives for studies of diversity, ecology, breeding and genomic programs aiming to understand deeply the biology of this species. We present a widely expressed gene catalog for T. grandis using Illumina technology and the de novo assembly. A total of 462,260 transcripts were obtained, with 1,502 and 931 genes differentially expressed for stem and branch secondary xylem, respectively, during age transition. Analysis of stem and branch secondary xylem indicates substantial similarity in gene ontologies including carbohydrate enzymes, response to stress, protein binding, and allowed us to find transcription factors and heat-shock proteins differentially expressed. TgMYB1 displays a MYB domain and a predicted coiled-coil (CC) domain, while TgMYB2, TgMYB3 and TgMYB4 showed R2R3-MYB domain and grouped with MYBs from several gymnosperms and flowering plants. TgMYB1, TgMYB4 and TgCES presented higher expression in mature secondary xylem, in contrast with TgMYB2, TgHsp1, TgHsp2, TgHsp3, and TgBi whose expression is higher in young lignified tissues. TgMYB3 is expressed at lower level in secondary xylem. Expression patterns of MYB transcription factors and heat-shock proteins in lignified tissues are dissimilar when tree development was evaluated, obtaining more expression of TgMYB1 and TgMYB4 in lignified tissues of 60-year-old trees, and more expression in TgHsp1, TgHsp2, Tg

  2. Trophobiosis in the arboricolous ant .i.Liometopum microcephalum./i. (Hymenoptera: Formicidae: Dolichoderinae)

    Czech Academy of Sciences Publication Activity Database

    Schlaghamerský, J.; Kašpar, J.; Petráková, L.; Šustr, Vladimír

    2013-01-01

    Roč. 110, č. 2 (2013), s. 231-239 ISSN 1210-5759 Institutional support: RVO:60077344 Keywords : Hymenoptera * arboricolous * ants * trophobiosis Subject RIV: EG - Zoology Impact factor: 1.076, year: 2013

  3. Two new species of the genus Peristenus Foerster (Hymenoptera: Braconidae: Euphorinae) from the Canary Islands

    NARCIS (Netherlands)

    Achterberg, van C.; Guerrero, E.R.

    2003-01-01

    Two new species of the genus Peristenus Foerster (Hymenoptera: Braconidae: Euphorinae) from the Canary Islands are described and illustrated: Peristenus angifemoralis spec. nov. from Tenerife, and P. gloriae spec. nov. from Gran Canaria and Tenerife.

  4. Semiochemicals from herbivory induced cotton plants enhance the foraging behavior of the cotton boll weevil, Anthonomus grandis.

    Science.gov (United States)

    Magalhães, D M; Borges, M; Laumann, R A; Sujii, E R; Mayon, P; Caulfield, J C; Midega, C A O; Khan, Z R; Pickett, J A; Birkett, M A; Blassioli-Moraes, M C

    2012-12-01

    The boll weevil, Anthonomus grandis, has been monitored through deployment of traps baited with aggregation pheromone components. However, field studies have shown that the number of insects caught in these traps is significantly reduced during cotton squaring, suggesting that volatiles produced by plants at this phenological stage may be involved in attraction. Here, we evaluated the chemical profile of volatile organic compounds (VOCs) emitted by undamaged or damaged cotton plants at different phenological stages, under different infestation conditions, and determined the attractiveness of these VOCs to adults of A. grandis. In addition, we investigated whether or not VOCs released by cotton plants enhanced the attractiveness of the aggregation pheromone emitted by male boll weevils. Behavioral responses of A. grandis to VOCs from conspecific-damaged, heterospecific-damaged (Spodoptera frugiperda and Euschistus heros) and undamaged cotton plants, at different phenological stages, were assessed in Y-tube olfactometers. The results showed that volatiles emitted from reproductive cotton plants damaged by conspecifics were attractive to adults boll weevils, whereas volatiles induced by heterospecific herbivores were not as attractive. Additionally, addition of boll weevil-induced volatiles from reproductive cotton plants to aggregation pheromone gave increased attraction, relative to the pheromone alone. The VOC profiles of undamaged and mechanically damaged cotton plants, in both phenological stages, were not different. Chemical analysis showed that cotton plants produced qualitatively similar volatile profiles regardless of damage type, but the quantities produced differed according to the plant's phenological stage and the herbivore species. Notably, vegetative cotton plants released higher amounts of VOCs compared to reproductive plants. At both stages, the highest rate of VOC release was observed in A. grandis-damaged plants. Results show that A. grandis uses

  5. CARACTERÍSTICAS TECNOLÓGICAS DA MADEIRA DE ÁRVORES MATRIZES DE Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Denis L. G. Fernandes

    2004-06-01

    Full Text Available O objetivo do presente trabalho foi caracterizar árvores matrizes de Eucalyptus grandis em relação a características tecnológicas da madeira. Foram selecionadas 63 árvores matrizes, pertencentes a um povoamento comercial localizado no litoral norte do Rio Grande do Sul. As árvores foram abatidas e, com base nos dados dendrométricos, calculou-se o volume comercial com e sem casca, o fator de forma, a conicidade e a relação altura/diâmetro. Quanto à madeira, foi analisada a massa específica básica, os percentuais volumétricos de cerne, alburno e casca, as rachaduras de topo das toras e das tábuas, os empenamentos e os defeitos visuais das tábuas, tais como nós e bolsa de resina. A massa específica básica, cuja média inclui a madeira dessa espécie entre as moderadamente leves a pesadas, mostrou uma tendência decrescente entre o DAP e 25% da altura comercial e, a partir daí, crescente até 100% da mesma. O percentual volumétrico de cerne apresentou valores médios crescentes desde a base até 25% da altura comercial e, a partir daí, diminuiu até 100% da altura comercial, sendo que a média geral foi de 75,7%. O comprimento médio das rachaduras de topo em tábuas de Eucalyptus grandis foi de 63%. A alta porcentagem de rachaduras de topo foi atribuída à posição de retirada das tábuas, próxima à medula. Já a variabilidade das rachaduras de topo encontrada para as toras (CV=60% possibilita a seleção de árvores com menor tendência em apresentar esse defeito.

  6. RNA-Seq reveals complex genetic response to deepwater horizon oil release in Fundulus grandis

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    Garcia Tzintzuni I

    2012-09-01

    Full Text Available Abstract Background The release of oil resulting from the blowout of the Deepwater Horizon (DH drilling platform was one of the largest in history discharging more than 189 million gallons of oil and subject to widespread application of oil dispersants. This event impacted a wide range of ecological habitats with a complex mix of pollutants whose biological impact is still not yet fully understood. To better understand the effects on a vertebrate genome, we studied gene expression in the salt marsh minnow Fundulus grandis, which is local to the northern coast of the Gulf of Mexico and is a sister species of the ecotoxicological model Fundulus heteroclitus. To assess genomic changes, we quantified mRNA expression using high throughput sequencing technologies (RNA-Seq in F. grandis populations in the marshes and estuaries impacted by DH oil release. This application of RNA-Seq to a non-model, wild, and ecologically significant organism is an important evaluation of the technology to quickly assess similar events in the future. Results Our de novo assembly of RNA-Seq data produced a large set of sequences which included many duplicates and fragments. In many cases several of these could be associated with a common reference sequence using blast to query a reference database. This reduced the set of significant genes to 1,070 down-regulated and 1,251 up-regulated genes. These genes indicate a broad and complex genomic response to DH oil exposure including the expected AHR-mediated response and CYP genes. In addition a response to hypoxic conditions and an immune response are also indicated. Several genes in the choriogenin family were down-regulated in the exposed group; a response that is consistent with AH exposure. These analyses are in agreement with oligonucleotide-based microarray analyses, and describe only a subset of significant genes with aberrant regulation in the exposed set. Conclusion RNA-Seq may be successfully applied to feral and

  7. Natural history of Hymenoptera venom allergy in Eastern Spain.

    Science.gov (United States)

    Fernandez, J; Soriano, V; Mayorga, L; Mayor, M

    2005-02-01

    The natural history of stings, the clinical reaction of the patient and in vivo and in vitro tests are necessary parameters to assess before initiating Hymenoptera venom immunotherapy. In the decision to initiate immunotherapy with Hymenoptera venom, it is not usual to evaluate the natural history of the disease, which seems to be self-limiting and therefore of variable clinical significance. Our aim was to determine the natural history of Hymenoptera hypersensitivity over 4 consecutive years in a rural Mediterranean population. An epidemiological study of Hymenoptera sting reactions and possible sensitivity was carried out in 145 randomly selected subjects out of a rural Mediterranean population of 600. Seventy-two subjects, including those with a history of anaphylaxis, completed the 4-year study. The nature of their clinical reactions, age, sex, history of atopy, profession, family history of reactions to Hymenoptera insects, time elapsed since the last sting, number of stings and specific IgE and IgG were determined (the latter, to the three most important insects in the area: Apis mellifera, Polistes dominulus, and Vespula germanica). Of the 72 subjects, four subjects had systemic reactions (SR), 23 had large local reaction (LLR) and all the others (117) was minor local reactions. None who had experienced an SR had a repeat SR when re-stung over the 4-year study. Of those with LLR, 12 subjects had the same type of reaction and 11 experienced more mild local reactions when re-stung. In the SR and local reaction groups, IgE to honey bee (Hb) increased significantly during the study period, whereas in those with only LLR, specific IgE to wasp (Polistes) decreased. Specific IgG to Polistes and Vespula (wasps) decreased significantly, whereas there was no change in the specific IgG to Hb in any of the groups. The number of stings per year decreased at the end of the study in all groups, but positive-specific IgG was higher in subjects with the greatest number of

  8. Durabilidad natural de madera de Eucalyptus grandis Hill ex Maiden de plantaciones de rápido crecimiento

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    Silvia Böthig

    2011-05-01

    Full Text Available Una de las especies forestales de rápido crecimiento cultivadas en Uruguay de mayor importancia económica es el Eucalyptus grandis. Trabajos anteriores reportan propiedades físicas y mecánicas de la madera juvenil y adulta proveniente de plantaciones de esta especie en diferentes regiones del país. Sin embargo, dado que no se dispone de datos científicos nacionales sobre su durabilidad natural, en este trabajo fue evaluada madera de E. grandis obtenida de dos plantaciones de 16 años de edad con semilla del mismo origen, de dos sitios, Rivera y Río Negro. Se estudió la durabilidad natural del duramen externo e interno siguiendo los métodos de la norma EN 350-1, tomando Populus deltoides x euroamericana cv I-214 como especie de referencia. Se realizaron ensayos de laboratorio para determinar la resistencia a la descomposición fúngica (Gloeophyllum trabeum, Trametes versicolor y Serpula lacrymans y a las termites (Reticulitermes spp. siguiendo las normas EN 113 y EN 118, respectivamente. Se realizaron ensayos de campo de estacas, de doble capa y cámara fúngica, los cuales aún están en curso, por lo que en el presente trabajo se presentan resultados parciales. Los perfiles radiales de densidad básica revelaron que la madera del duramen externo no era adulta, sino madera de transición. En general, el duramen de E. grandis mostró una mayor durabilidad que el híbrido Populus.El E. grandis se clasificó como moderadamente o seriamente atacado por Reticulitermes spp. Según EN 350-1, la madera juvenil de E. grandis puede considerarse como “moderadamente durable” ante la pudrición parda provocada por G. trabeum, mientras que la madera de transición puede describirse como “durable”. La madera de transición mostró en relación a la madera juvenil una mayor resistencia al G. trabeum, una susceptibilidad levemente menor a las termites y mejor desempeño en el campo luego de 17 meses de exposición. El sitio de la plantación no

  9. Spatial Analysis of Agricultural Landscape and Hymenoptera Biodiversity at Cianjur Watershed

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    YAHERWANDI

    2006-12-01

    Full Text Available Hymenoptera is one of the four largest insect order (the other three are Coleoptera, Diptera, and Lepidoptera. There are curerently over 115 000 described Hymenoptera species. It is clear that Hymenoptera is one of the major components of insect biodiversity. However, Hymenoptera biodiversity is affected by ecology, environment, and ecosystem management. In an agricultural areas, the spatial structure, habitat diversity, and habitat composition may vary from cleared landscapes to structurally rich landscape. Thus, it is very likely that such large-scale spatial patterns (landscape effects may influence local biodiversity and ecological functions. Therefore, the objective of this research were to study diversity and configuration elements of agricultural landscapes at Cianjur Watershed with geographical information sytems (GIS and its influence on Hymenoptera biodiversity. The structural differences between agricultural landscapes of Nyalindung, Gasol, and Selajambe were characterized by patch analyst with ArcView 3.2 of digital land use data. Results indicated that class of land uses of Cianjur Watershed landscape were housing, mixed gardens, talun and rice, vegetable, and corn fields. Landscape structure influenced the biodiversity of Hymenoptera. Species richness and the species diversity were higher in Nyalindung landscape compare to Gasol and Selajambe landscape.

  10. Pengaruh Habitat Sekitar Lahan Persawahan dan Umur Tanaman Padi terhadap Keanekaragaman Hymenoptera Parasitika

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    Nina Herlina

    2015-09-01

    Full Text Available As the largest group of biological control agents, Parasitic Hymenoptera play important role in controlling pest outbreak in agricultural habitat. Unfortunately, there is lack of information about how these parasitoids occur in agricultural habitat related to condition of surrounding habitat and phenology of crop plant. The objective of this research was to study the effect of rice field surrounding habitat and age of rice plant on the diversity of Parasitic Hymenoptera. Research area was located in Carang Pulang Village, Dramaga, Bogor. We selected four blocks which represented different of habitat condition and age of rice plant. Each block was set six yellow pan traps (with minimum distance 20 meter and one malaise trap. Sampling of insects were conducted weekly from 6 to 12 week after planting. From this research, we collected 1,833 individual of Hymenoptera (without ants belong to 9 superfamilies, 23 families, and 216 species. Parasitic Hymenoptera was more abundant (96% and species rich (84% than Aculeata. Rice field surrounding habitat (block and age of rice field significantly affect the diversity of Parasitic Hymenoptera. We found positively correlation between age of rice plant and species richness of Parasitic Hymenoptera.

  11. Taxonomic and functional responses to fire and post-fire management of a Mediterranean hymenoptera community.

    Science.gov (United States)

    Mateos, Eduardo; Santos, Xavier; Pujade-Villar, Juli

    2011-11-01

    Fire is one of the commonest disturbances worldwide, transforming habitat structure and affecting ecosystem functioning. Understanding how species respond to such environmental disturbances is a major conservation goal that should be monitored using functionally and taxonomically diverse groups such as Hymenoptera. In this respect, we have analyzed the taxonomic and functional response to fire and post-fire management of a Hymenoptera community from a Mediterranean protected area. Thus, Hymenoptera were sampled at fifteen sites located in three burnt areas submitted to different post-fire practices, as well as at five sites located in peripheral unburnt pine forest. A total of 4882 specimens belonging to 33 families, which were classified into six feeding groups according to their dietary preferences, were collected. ANOVA and Redundancy Analyses showed a taxonomic and functional response to fire as all burnt areas had more Hymenoptera families, different community composition and higher numbers of parasitoids than the unburnt area. Taxonomic differences were also found between burnt areas in terms of the response of Hymenoptera to post-fire management. In general the number of parasitoids was positively correlated to the number of potential host arthropods. Parasitoids are recognized to be sensitive to habitat changes, thus highlighting their value for monitoring the functional responses of organisms to habitat disturbance. The taxonomic and functional responses of Hymenoptera suggest that some pine-forest fires can enhance habitat heterogeneity and arthropod diversity, hence increasing interspecific interactions such as those established by parasitoids and their hosts.

  12. Taxonomic and Functional Responses to Fire and Post-Fire Management of a Mediterranean Hymenoptera Community

    Science.gov (United States)

    Mateos, Eduardo; Santos, Xavier; Pujade-Villar, Juli

    2011-11-01

    Fire is one of the commonest disturbances worldwide, transforming habitat structure and affecting ecosystem functioning. Understanding how species respond to such environmental disturbances is a major conservation goal that should be monitored using functionally and taxonomically diverse groups such as Hymenoptera. In this respect, we have analyzed the taxonomic and functional response to fire and post-fire management of a Hymenoptera community from a Mediterranean protected area. Thus, Hymenoptera were sampled at fifteen sites located in three burnt areas submitted to different post-fire practices, as well as at five sites located in peripheral unburnt pine forest. A total of 4882 specimens belonging to 33 families, which were classified into six feeding groups according to their dietary preferences, were collected. ANOVA and Redundancy Analyses showed a taxonomic and functional response to fire as all burnt areas had more Hymenoptera families, different community composition and higher numbers of parasitoids than the unburnt area. Taxonomic differences were also found between burnt areas in terms of the response of Hymenoptera to post-fire management. In general the number of parasitoids was positively correlated to the number of potential host arthropods. Parasitoids are recognized to be sensitive to habitat changes, thus highlighting their value for monitoring the functional responses of organisms to habitat disturbance. The taxonomic and functional responses of Hymenoptera suggest that some pine-forest fires can enhance habitat heterogeneity and arthropod diversity, hence increasing interspecific interactions such as those established by parasitoids and their hosts.

  13. Química e colorimetria da madeira de Eucalyptus grandis W. Mill ex Maiden termorretificada

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    Antônio José Vinha Zanuncio

    2014-08-01

    Full Text Available A termorretificação visa melhorar as características para agregar valor à madeira através da aplicação de calor. O objetivo foi avaliar as alterações químicas e colorimétricas da madeira termorretificada de Eucalyptus grandis submetida a 170, 200 e 230 ºC por 3, 5 e 7 h. Os teores de extrativos, lignina, holocelulose, composição elementar e os parâmetros colorimétricos foram determinados. Os teores de extrativos aumentaram nos tratamentos a 170 °C e diminuíram a partir de 200 °C, enquanto o teor de lignina total aumentou e o de holocelulose diminuiu a partir de 200 °C. O teor de carbono aumentou e o de oxigênio diminuiu a 230 °C por 5 e 7 h. A termorretificação reduziu a claridade, matriz vermelho (a* e matriz amarelo (b* da madeira de eucalipto em todos os tratamentos.

  14. Cellulose nanocrystal from pomelo (C. Grandis osbeck) albedo: Chemical, morphology and crystallinity evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Zain, Nor Fazelin Mat; Yusop, Salma Mohamad [Food Science Program, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor (Malaysia); Ahmad, Ishak [Polymer Research Centre (PORCE), School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor (Malaysia)

    2013-11-27

    Citrus peel is one of the under-utilized waste materials that have potential in producing a valuable fibre, which are cellulose and cellulose nanocrystal. Cellulose was first isolated from pomelo (C. Grandis Osbeck) albedo by combination of alkali treatment and bleaching process, followed by acid hydrolysis (65% H{sub 2}SO{sub 4}, 45 °C, 45min) to produce cellulose nanocrystal. The crystalline, structural, morphological and chemical properties of both materials were studied. Result reveals the crystallinity index obtained from X-ray diffraction for cellulose nanocrystal was found higher than extracted cellulose with the value of 60.27% and 57.47%, respectively. Fourier transform infrared showed that the chemical treatments removed most of the hemicellulose and lignin from the pomelo albedo fibre. This has been confirmed further by SEM and TEM for their morphological studies. These results showed that cellulose and cellulose nanocrystal were successfully obtained from pomelo albedo and might be potentially used in producing functional fibres for food application.

  15. Molecular diagnosis of populational variants of Anthonomus grandis (Coleoptera: Curculionidae) in North America.

    Science.gov (United States)

    Barr, Norman; Ruiz-Arce, Raul; Obregón, Oscar; De Leon, Rosita; Foster, Nelson; Reuter, Chris; Boratynski, Theodore; Vacek, Don

    2013-02-01

    The utility of the cytochrome oxidase I (COI) DNA sequence used for DNA barcoding and a Sequence Characterized Amplified Region for diagnosing boll weevil, Anthonomus grandis Boheman, variants was evaluated. Maximum likelihood analysis of COI DNA sequences from 154 weevils collected from the United States and Mexico supports previous evidence for limited gene flow between weevil populations on wild cotton and commercial cotton in northern Mexico and southern United States. The wild cotton populations represent a variant of the species called the thurberia weevil, which is not regarded as a significant pest. The 31 boll weevil COI haplotypes observed in the study form two distinct haplogroups (A and B) that are supported by five fixed nucleotide differences and a phylogenetic analysis. Although wild and commercial cotton populations are closely associated with specific haplogroups, there is not a fixed difference between the thurberia weevil variant and other populations. The Sequence Characterized Amplified Region marker generated a larger number of inconclusive results than the COI gene but also supported evidence of shared genotypes between wild and commercial cotton weevil populations. These methods provide additional markers that can assist in the identification of pest weevil populations but not definitively diagnose samples.

  16. Isolation and characterization of farnesyl diphosphate synthase from the cotton boll weevil, Anthonomus grandis.

    Science.gov (United States)

    Taban, A Huma; Tittiger, Claus; Blomquist, Gary J; Welch, William H

    2009-06-01

    Farnesyl diphosphate synthase (FPPS) catalyzes the consecutive condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate to form farnesyl diphosphate (FPP). In insects, FPP is used for the synthesis of ubiquinones, dolicols, protein prenyl groups, and juvenile hormone. A full-length cDNA of FPPS was cloned from the cotton boll weevil, Anthonomus grandis (AgFPPS). AgFPPS cDNA consists of 1,835 nucleotides and encodes a protein of 438 amino acids. The deduced amino acid sequence has high similarity to previously isolated insect FPPSs and other known FPPSs. Recombinant AgFPPS expressed in E. coli converted labeled isopentenyl diphosphate in the presence of dimethylallyl diphosphate to FPP. Southern blot analysis indicated the presence of a single copy gene. Using molecular modeling, the three-dimensional structure of coleopteran FPPS was determined and compared to the X-ray crystal structure of avian FPPS. The alpha-helical fold is conserved in AgFPPS and the size of the active site cavity is consistent with the enzyme being a FPPS. (c) 2009 Wiley Periodicals, Inc.

  17. Collection of pheromone from atmosphere surrounding boll weevils,Anthonomus grandis.

    Science.gov (United States)

    Chang, J F; Benedict, J H; Payne, T L; Camp, B J; Vinson, S B

    1989-02-01

    An effluvial method was developed to collect the pheromone, grandlure from actively calling male boll weevils,Anthonomus grandis Boheman. The adsorbant, Porapak Q (ethylvinylbenzene-divinylbenzene), was utilized to trap and concentrate the pheromone. Captured pheromone was desorbed from columns packed with Porapak Q by elution withn-pentane and quantified by capillary column gas-liquid chromatography. In recovery studies with known amounts of synthetic grandlure, we found that the amount of each pheromone component collected was a function of collection duration, elution volume, and initial concentration. This effluvial method was capable of recovering as much as 94.9% of a known quantity (80 μg) of grandlure. The chromatograms were free of extraneous peaks. In studies of insect-produced pheromone, the effluvial method was used to collect pheromone from the air space surrounding male boll weevils as they fed on flower buds from CAMD-E cotton. The quantity and quality of boll-weevil-produced pheromone was determined for days 6, 8, 10, 11, 12, 13, and 14 of boll weevil adulthood. The maximum quantity of natural pheromone was produced on day 13 (4.2 μg/weevil) with a pheromone component ratio of 2.41∶2.29∶0.95∶1 for components I, II, III, and IV, respectively. The effluvial method described in this report is an efficient method to collect and quantify boll weevil pheromone from the atmosphere surrounding actively calling insects. Other applications of this method are suggested.

  18. Olfaction in the boll weevil,Anthonomus grandis Boh. (Coleoptera: Curculionidae): Electroantennogram studies.

    Science.gov (United States)

    Dickens, J C

    1984-12-01

    Electroantennogram (EAG) techniques were utilized to measure the antennal olfactory responsiveness of adult boll weevils,Anthonomus grandis Boh. (Coleoptera: Curculionidae), to 38 odorants, including both insect and host plant (Gossypium hirsutum L.) volatiles. EAGs of both sexes were indicative of at least two receptor populations: one receptor population primarily responsive to pheromone components and related compounds, the other receptor population primarily responsive to plant odors. Similar responses to male aggregation pheromone components (i.e., compounds I, II, and III + IV) were obtained from both sexes, but females were slightly more sensitive to I. Both sexes were highly responsive to components of the "green leaf volatile complex," especially the six-carbon saturated and monounsaturated primary alcohols. Heptanal was the most active aldehyde tested. More acceptors responded to oxygenated monoterpenes than to monoterpene hydrocarbons. β-Bisabolol, the major volatile of cotton, was the most active sesquiterpene. In general, males, which are responsible for host selection and pheromone production, were more sensitive to plant odors than were females. In fact, males were as sensitive to β-bisabolol and heptanal as to aggregation pheromone components. Electrophysiological data are discussed with regard to the role of insect and host plant volatiles in host selection and aggregation behavior of the boll weevil.

  19. Infestation of Anthonomus grandis (Coleoptera: Curculionidae on re-sprout of cotton plants

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    José Fernando Jurca Grigolli

    2015-06-01

    Full Text Available The destruction of cotton crop residues at the end of the crop cycle is a key strategy for the phytosanitary crop management, since its off-season re-sprout can provide sites for feeding and oviposition of pests such as the boll weevil. This study aimed to evaluate the re-sprout capacity of cotton cultivars, as well as their infestation by Anthonomus grandis. A randomized blocks design, in a 3 x 2 factorial arrangement, with three cultivars (FM 910, DeltaOPAL and NuOPAL, two mowing heights (10 cm and 20 cm and four replications, was used. Weekly evaluations were carried out for measuring the percentage of plant re-sprout for both mowing heights, percentage of flower buds infested by the boll weevil and number of adults per re-sprout. Plants mowed at 10 cm presented a lower sprout capacity and consequently less flower buds, reducing the boll weevil population density in the area, while plants mowed at 20 cm showed high re-sprouts rates, seven days after mowing. The FM 910 cultivar had the highest number of re-sprout plants, while the DeltaOPAL cultivar showed the highest number of flower buds and adults per plant, as well as the highest percentage of buds damaged by the boll weevil.

  20. Structure and Identification of Solenin: A Novel Fibrous Protein from Bivalve Solen grandis Ligament

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    Jun Meng

    2014-01-01

    Full Text Available Fibrous proteins, which derived from natural sources, have been acting as templates for the production of new materials for decades, and most of them have been modified to improve mechanical performance. Insight into the structures of fibrous proteins is a key step for fabricating of bioinspired materials. Here, we revealed the microstructure of a novel fibrous protein: solenin from Solen grandis ligament and identified the protein by MALDI-TOF-TOF-MS and LC-MS-MS analyses. We found that the protein fiber has no hierarchical structure and is homologous to keratin type II cytoskeletal 1 and type I cytoskeletal 9-like, containing “SGGG,” “SYGSGGG,” “GS,” and “GSS” repeat sequences. Secondary structure analysis by FTIR shows that solenin is composed of 41.8% β-sheet, 16.2% β-turn, 26.5% α-helix, and 9.8% disordered structure. We believe that the β-sheet structure and those repeat sequences which form “glycine loops” may give solenin excellence elastic and flexible properties to withstand tensile stress caused by repeating opening and closing of the shell valves in vivo. This paper contributes a novel fibrous protein for the protein materials world.

  1. The GC/MS Analysis of Volatile Components Extracted by Different Methods from Exocarpium Citri Grandis

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    Zhisheng Xie

    2013-01-01

    Full Text Available Volatile components from Exocarpium Citri Grandis (ECG were, respectively, extracted by three methods, that is, steam distillation (SD, headspace solid-phase microextraction (HS-SPME, and solvent extraction (SE. A total of 81 compounds were identified by gas chromatography-mass spectrometry including 77 (SD, 56 (HS-SPME, and 48 (SE compounds, respectively. Despite of the extraction method, terpenes (39.98~57.81% were the main volatile components of ECG, mainly germacrene-D, limonene, 2,6,8,10,14-hexadecapentaene, 2,6,11,15-tetramethyl-, (E,E,E-, and trans-caryophyllene. Comparison was made among the three methods in terms of extraction profile and property. SD relatively gave an entire profile of volatile in ECG by long-time extraction; SE enabled the analysis of low volatility and high molecular weight compounds but lost some volatiles components; HS-SPME generated satisfactory extraction efficiency and gave similar results to those of SD at analytical level when consuming less sample amount, shorter extraction time, and simpler procedure. Although SD and SE were treated as traditionally preparative extractive techniques for volatiles in both small batches and large scale, HS-SPME coupled with GC/MS could be useful and appropriative for the rapid extraction and qualitative analysis of volatile components from medicinal plants at analytical level.

  2. Laser light interaction for texture evaluation of native woods and Eucalyptus grandis W. Hill ex Maiden

    International Nuclear Information System (INIS)

    Silva, M.R.; Mori, F.A.; Rabelo, G.F.; Braga Junior, R.A.

    2005-01-01

    This work had the objective of study in patterns of the ''speckle'' as function of the wood texture of Bowdichia virgilioides check for this species in other resources (sucupira), Swietenia macrophylla check for this species in other resources (mogno), Balfourodendron riedelianum check for this species in other resources (paumarfim) and Eucalyptus grandis wood. The He-Ne laser 10 mW was used to illuminate the wood samples and a digital camera captured the result of the interaction of the wood with the laser light. The results obtained from image analysis were compared with the pattern obtained by microscopic methodology. The captured images show the intensities of each pixel, varying between 0 and 255 in the gray scale. From the images, a central area of 16x16 pixels was removed and analyzed by the application of the convolution function and its Fourier Transform, resulting in the power density spectrum. The results showed that the information obtained by the power density spectrum of the ''speckle'' space variation allow classing samples of woods with different texture [pt

  3. PARTICLEBOARDS PRODUCED WITH Eucalyptus grandis W. Hill ex Maiden POLYSTYRENE AND POLYETHYLENE THEREPHTHALATE PARTICLES

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    Antônio da Silva Maciel

    2004-06-01

    Full Text Available This research investigated the properties of wood particleboards, containing particles of polystyrene (PS and polyethylene therephthalate (PET. Particleboards were produced with 0%, 25% or 50% of polystyrene and 100%, 75% or 50% of Eucalyptus grandis particles. Additional boards were produced with two amounts of PET/PS (5/20% or 10/40% and 75% or 50% wood particles. As binding agents three amounts (0%, 4% or 6% of urea-formaldehyde or phenol-formaldehyde adhesive and three amounts of polystyrene in toluene solution (0%, 4% or 6% were used. One-layer boards, measuring approximately 400,0 x 400,0 x 10,0 mm with density approximately equal to 0,60 g/cm3, were produced. Internal bond, modulus of rupture and elasticity, screw withdrawal, as well water absorption and thickness swelling, after 24 hours of immersion were determined. All mechanical boards’ properties were superior to the established by ANSI/A 208.1-1993 standards. All wood/plastic particleboards absorbed more water then those observed in commercial boards. However the observed thickness swelling was quite similar to the values of commercial wood particleboards. Boards in which the solution of polystyrene was applied were, in general, presented the best values for all properties.

  4. Ekstraksi Dan Karakterisasi Serbuk Nano Pigmen Dari Daun Tanaman Jati (Tectona Grandis Linn. F

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    Riahna br Kembaren

    2014-04-01

    Full Text Available Tanaman Jati (Tectona grandis linn. F umumnya hanya dimanfaatkan bagian kayunya untuk industri meubel, namun bagian lain seperti daun kurang dimanfaatkan. Daun jati terutama bagian pucuk daun muda dapat menghasilkan pigmen. Produksi serbuk nano pigmen dari daun jati dan karakterisasi serbuk nano pigmen tersebut belum dilakukan. Tujuan penelitian ini adalah menghasilkan nano pigmen dari pucuk daun jati muda dalam bentuk serbuk dengan menggunakan persentase filler yang berbeda dan melakukan karakterisasi serbuk nano pigmen jati tersebut. Pucuk daun jati muda diberi perlakuan mekanik dengan penggerusan kemudian disaring, larutan yang diperoleh diukur partikelnya dengan Particle Size Analyzer (PSA, dan dikeringkan dengan penambahan filler maltodekstrin 5% dan 10%. Serbuk yang diperoleh dihitung rendemen, ukuran partikel, dan kelarutan dalam air. Warna merah yang dihasilkan dari filtrat pucuk daun jati muda berasal dari zat warna antosianin yang terkandung dalam daun jati muda. Ekstrak dari pucuk daun jati muda memiliki ukuran dengan kisaran 87,8- 318,1 nm dengan ukuran rata-rata 109,2 nm. Hal ini menunjukkan bahwa ekstrak tersebut merupakan produk nano di alam. Penambahan filler dengan konsentrasi berbeda berpengaruh terhadap warna, rendemen, ukuran partikel serbuk, dan kelarutan pigmen serbuk dalam air. 

  5. STORAGE TIME EFFECT ON MINI-CUTTINGS ROOTING IN Tectona grandis LINN F. CLONES

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    Yorleny Badilla

    2017-08-01

    Full Text Available ABSTRACT The study aimed to evaluate the influence of storage length on Tectona grandis mini-cuttings survival and rooting. A factorial arrangement (4 x 7 was utilized, based on four clones (Carapá, Ipê, GU5 and TB7 and seven time intervals from mini-cuttings harvesting until final sowing (0, 1, 2, 4, 8, 12 and 16 hours. A randomized block design with three replicates and 16 mini-cuttings per experimental unit was utilized. Survival and rooting rates were evaluated after greenhouse culture (30 days after sowing and after shadow house culture (40 days after sowing; as well as height, collar diameter, aerial and root biomass 55 days after sowing. No significant differences were observed in survival and rooting rates among time intervals in teak mini-cuttings preparation from these four clones. However differences among clones were registered for rooting rate, suggesting a genotypic effect. Survival and rooting rates were very high after greenhouse culture (93% and 90% respectively, as well as survival after culture in a shadow-house (88%.

  6. Multigene families encode the major enzymes of antioxidant metabolism in Eucalyptus grandis L

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    Felipe Karam Teixeira

    2005-01-01

    Full Text Available Antioxidant metabolism protects cells from oxidative damage caused by reactive oxygen species (ROS. In plants, several enzymes act jointly to maintain redox homeostasis. Moreover, isoform diversity contributes to the fine tuning necessary for plant responses to both exogenous and endogenous signals influencing antioxidant metabolism. This study aimed to provide a comprehensive view of the major classes of antioxidant enzymes in the woody species Eucalyptus grandis. A careful survey of the FORESTs data bank revealed 36 clusters as encoding antioxidant enzymes: six clusters encoding ascorbate peroxidase (APx isozymes, three catalase (CAT proteins, three dehydroascorbate reductase (DHAR, two glutathione reductase (GR isozymes, four monodehydroascorbate reductase (MDHAR, six phospholipid hydroperoxide glutathione peroxidases (PhGPx, and 12 encoding superoxide dismutases (SOD isozymes. Phylogenetic analysis demonstrated that all clusters (identified herein grouped with previously characterized antioxidant enzymes, corroborating the analysis performed. With respect to enzymes involved in the ascorbate-glutathione cycle, both cytosolic and chloroplastic isoforms were putatively identified. These sequences were widely distributed among the different ESTs libraries indicating a broad gene expression pattern. Overall, the data indicate the importance of antioxidant metabolism in eucalyptus.

  7. Características de vigas laminadas coladas confeccionadas com madeira de teca (Tectona grandis

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    Setsuo Iwakiri

    Full Text Available O objetivo desta pesquisa foi avaliar a influência do adesivo, das gramaturas e dos tempos de prensagem nas propriedades mecânicas de vigas laminadas coladas, confeccionadas com madeira de teca (Tectona grandis. As vigas foram produzidas com cinco lamelas, sendo duas da classe de módulo de elasticidade dinâmico, classe 1 - MOEd C1 nas faces, e três da classe 2 - MOEd C2 no miolo. Para a colagem, utilizou-se o adesivo à base de resorcina-fenol-formaldeído (RFF, com gramaturas de 350 e 450 g.m- 2, e tempos de prensagem de 5 e 8 horas. Para o adesivo à base de emulsão polimérica de isocianato (EPI, a colagem foi realizada com gramaturas de 180 e 220 g.m- 2, e tempos de prensagem de 3 e 4 horas. Os resultados das propriedades mecânicas das vigas indicaram que não houve uma relação bem definida dos efeitos das variáveis de estudo nas propriedades de flexão estática. As vigas coladas com o adesivo RFF tiveram maior resistência da linha de cola em comparação às vigas coladas com o adesivo EPI.

  8. Vergamento da madeira de Eucalyptus grandis plasticizada por vaporização e cozimento

    Directory of Open Access Journals (Sweden)

    Matheus Lemos Peres

    Full Text Available O vergamento de madeira foi utilizado ao longo dos séculos para a produção de objetos grandes, como estruturas de embarcações ou pequenos utensílios. Mesmo com constantes estudos de vergamento, há carência de melhorias de processo e inclusão de madeiras de baixo valor comercial. Assim, o presente trabalho analisou a madeira vergada de Eucalyptus grandis tratada por diferentes tempos de exposição à vaporização e ao cozimento. Foram analisados o número de tipos de defeitos por peça vergada (Nºdef, a variação no teor de umidade perante os tratamentos (∆Tu, a pressão de extremidade (Pe e a perda de forma circular (Pf. Verificou-se que as variáveis diferem significativamente entre os tipos de tratamento de vaporização e cozimento, exceto para o Nºdef. Os tempos de tratamento não apresentaram efeito significativo. Concluiu-se que a espécie é aceitável para vergamentos em raios inferiores ao utilizado no presente estudo. Adicionalmente, a vaporização mostrou ser o melhor tratamento, por apresentar melhores parâmetros de qualificação.

  9. Structure and function of the digestive system of solen grandis dunker

    Science.gov (United States)

    Sheng, Xiuzhen; Zhan, Wenbin; Ren, Sulian

    2003-10-01

    Structure and function of the digestive system of a bivalve mollusc, Solen grandis, were studied using light microscopy and histochemical methods. The wall of digestive tube consists of four layers: the mucosal epithelium, connective tissue, muscular and fibrosa or serosa (only in the portion of rectum) from the inner to the outer. The ciliated columnar epithelial cells, dispersed by cup-shaped mucous cells, rest on a thin base membrane. There are abundant blood spaces in connective tissue layer. The digestive diverticula are composed of multi-branched duct and digestive tubules. The digestive tubules are lined with digestive and basophilic secretory cells, and surrounded by a layer of smooth muscle fibers and connective tissues. Activities of acid and alkaline phosphatases, esterase and lipase are detected in the digestive cells, and the epithelia of stomach and intestine, suggesting that these cells are capable of intracellular digesting of food materials and absorbing. Besides, acid phosphatase and esterase activities are present in the posterior portion of esophagus. Phagocytes are abundant in blood spaces and the lumens of stomach and intestine, containing brown granules derived from the engulfed food materials. The present work indicates that phagocytes play important roles in ingestion and digestion of food materials, which is supported as well by the activities of acid phosphatase, esterase and lipase detected in blood spaces.

  10. RELATIONSHIP BETWEEN THE ANATOMY AND DRYING IN Eucalyptus grandis X Eucalyptus urophylla WOOD

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    Antônio José Vinha Zanuncio

    Full Text Available ABSTRACT Drying is an important step to using wood and anatomical characteristics influence this process. Thus, the objective of this work was to evaluate the relationship between anatomy and wood drying. Samples with 2 x 2 x 4 cm were obtained from eight Eucalyptus urophylla x Eucalyptus grandis clones at 1.3 m height to evaluate the anatomy and drying in climate chamber during 15 days. Cell wall fraction was established as the parameter that best correlated with drying, the Pearson correlation coefficient between this parameter and moisture was -0.8986, -0.6580, 0.9216, 0.8743, 0.7131 and 0.8727 for saturated wood, and after 1, 2.5, 5, 10 and 15 days of drying, respectively. The frequency and vessel size, as well as the height and width of the rays showed low relation with wood drying. Wood anatomy, mainly the cell wall fraction, influences moisture losses and should be considered in wood drying programs.

  11. Effect of fertilization on cell size in wood of Eucalyptus grandis Hill Ex Maiden

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    Luiz Santini Junior

    2010-12-01

    Full Text Available The use of fertilization in forest stands results in yield gains, yet little attention has been directed to its potential effects on the quality of wood produced. Information is scarce about the effect of fertilization on anatomical structures of older Eucalyptus wood. This work aims to study the effect of fertilization on tissue cell size of wood from a Eucalyptus grandis stand at age 21 years, the management system of which is based on selective thinning and fertilizer application at the start of the thinning season. Factors to consider include: presence or absence of fertilizers, two log positions and five radial (pith to bark positions. Results led to the conclusion that fertilization significantly influenced only vessel frequency. Vessel elements length was influenced by tree height. Fiber length, fiber diameter, fiber wall thickness, vessel elements length, vessel diameter and vessel frequency were influenced by the radial position of the sample in relation to the log. A positive correlation was observed between fiber length, fiber diameter, fiber wall thickness, vessel elements length, vessel diameter, ray width and radial position, while a negative correlation was observed between ray frequency and radial position.

  12. Volatile constituents of redblush grapefruit (Citrus paradisi) and pummelo (Citrus grandis) peel essential oils from Kenya.

    Science.gov (United States)

    Njoroge, Simon Muhoho; Koaze, Hiroshi; Karanja, Paul Nyota; Sawamura, Masayoshi

    2005-12-14

    The volatile constituents of cold-pressed peel essential oils of redblush grapefruit (Citrus paradisi Macfadyen forma Redblush) and pummelo (Citrus grandis Osbeck) from the same locality in Kenya were determined by GC and GC-MS. A total of 67 and 52 compounds, amounting to 97.9 and 98.8% of the two oils, respectively, were identified. Monoterpene hydrocarbons constituted 93.3 and 97.5% in the oils, respectively, with limonene (91.1 and 94.8%), alpha-terpinene (1.3 and 1.8%), and alpha-pinene (0.5%) as the main compounds. Sesquiterpene hydrocarbons constituted 0.4% in each oil. The notable compounds were beta-caryophyllene, alpha-cubebene, and (E,E)-alpha-farnesene. Oxygenated compounds constituted 4.2 and 2.0% of the redblush grapefruit and pummelo oils, respectively, out of which carbonyl compounds (2.0 and 1.3%), alcohols (1.4 and 0.3%), and esters (0.7 and 0.4%) were the major groups. Heptyl acetate, octanal, decanal, citronellal, and (Z)-carvone were the main constituents (0.1-0.5%). Perillene, (E)-carveol, and perillyl acetate occurred in the redblush grapefruit but were absent from the pummelo oil. Nootkatone, alpha- and beta-sinensal, methyl-N-methylanthranilate, and (Z,E)-farnesol were prominent in both oils.

  13. Applying Sewage Sludge to Eucalyptus grandis Plantations: Effects on Biomass Production and Nutrient Cycling through Litterfall

    International Nuclear Information System (INIS)

    Da Silva, P.H.M.; Poggiani, F.; Laclau, J.P.

    2011-01-01

    In most Brazilian cities sewage sludge is dumped into sanitary landfills, even though its use in forest plantations as a fertilizer and soil conditioner might be an interesting option. Sewage sludge applications might reduce the amounts of mineral fertilizers needed to sustain the productivity on infertile tropical soils. However, sewage sludge must be applied with care to crops to avoid soil and water pollution. The aim of our study was to assess the effects of dry and wet sewage sludges on the growth and nutrient cycling of Eucalyptus grandis plantations established on the most common soil type for Brazilian eucalypt plantations. Biomass production and nutrient cycling were studied over a 36-month period in a complete randomized block design. Four experimental treatments were compared: wet sewage sludge, dry sludge, mineral fertilizer, and no fertilizer applications. The two types of sludges as well as mineral fertilizer increased significantly the biomass of Eucalyptus trees. Wood biomass productions 36 months after planting were similar in the sewage sludge and mineral fertilization treatments (about 80 tons ha - '1) and 86 % higher than in the control treatment. Sewage sludge application also affected positively leaf litter production and significantly increased nutrient transfer among the components of the ecosystem.

  14. Leaf area index estimation of Eucalyptus grandis W.Hill. in plantations

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    Dubal Papamija-Muñoz

    2012-12-01

    Full Text Available We estimated leaf area index (LAI in Eucalyptus grandis W.Hill. plantations in four farms in the Smurfit Kappa Carton de Colombia (SKCC with three farms located in the city of Popayan (Cauca and one located in the municipality of Restrepo (Valle del Cauca. Each farm had three fertilized and three unfertilized plots with 64 individuals in each. We used three methods, Plant Canopy Analyzer 2000 (PCA 2000, flat photograph PIPEcv software and a destructive method, which was generated using a mathematical model. The first two methods were measured bimonthly for a year and the final method required trees being cut to measure their diameter. Estimation of leaf area index was 2.01 for PCA 2000, 3.12 for PIPEcv and 2.83 for the mathematical model. These values correspond to the average and range of leaf area indices obtained for each method on all farms. Statistically the three methodologies developed in this study were not closely related.

  15. Cellulose nanocrystal from pomelo (C. Grandis osbeck) albedo: Chemical, morphology and crystallinity evaluation

    International Nuclear Information System (INIS)

    Zain, Nor Fazelin Mat; Yusop, Salma Mohamad; Ahmad, Ishak

    2013-01-01

    Citrus peel is one of the under-utilized waste materials that have potential in producing a valuable fibre, which are cellulose and cellulose nanocrystal. Cellulose was first isolated from pomelo (C. Grandis Osbeck) albedo by combination of alkali treatment and bleaching process, followed by acid hydrolysis (65% H 2 SO 4 , 45 °C, 45min) to produce cellulose nanocrystal. The crystalline, structural, morphological and chemical properties of both materials were studied. Result reveals the crystallinity index obtained from X-ray diffraction for cellulose nanocrystal was found higher than extracted cellulose with the value of 60.27% and 57.47%, respectively. Fourier transform infrared showed that the chemical treatments removed most of the hemicellulose and lignin from the pomelo albedo fibre. This has been confirmed further by SEM and TEM for their morphological studies. These results showed that cellulose and cellulose nanocrystal were successfully obtained from pomelo albedo and might be potentially used in producing functional fibres for food application

  16. Applying Sewage Sludge to Eucalyptus grandis Plantations: Effects on Biomass Production and Nutrient Cycling through Litterfall

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    Paulo Henrique Müller da Silva

    2011-01-01

    Full Text Available In most Brazilian cities sewage sludge is dumped into sanitary landfills, even though its use in forest plantations as a fertilizer and soil conditioner might be an interesting option. Sewage sludge applications might reduce the amounts of mineral fertilizers needed to sustain the productivity on infertile tropical soils. However, sewage sludge must be applied with care to crops to avoid soil and water pollution. The aim of our study was to assess the effects of dry and wet sewage sludges on the growth and nutrient cycling of Eucalyptus grandis plantations established on the most common soil type for Brazilian eucalypt plantations. Biomass production and nutrient cycling were studied over a 36-month period in a complete randomized block design. Four experimental treatments were compared: wet sewage sludge, dry sludge, mineral fertilizer, and no fertilizer applications. The two types of sludges as well as mineral fertilizer increased significantly the biomass of Eucalyptus trees. Wood biomass productions 36 months after planting were similar in the sewage sludge and mineral fertilization treatments (about 80 tons ha−1 and 86% higher than in the control treatment. Sewage sludge application also affected positively leaf litter production and significantly increased nutrient transfer among the components of the ecosystem.

  17. Palmistichus elaeisis Delvare & LaSalle (Hymenoptera, Eulophidae: a new parasitoid of Dione juno juno (Cramer (Lepidoptera, Nymphalidae Palmistichus elaeisis Delvare & LaSalle (Hymenoptera, Eulophidae: um novo parasitóide de Dione juno juno (Cramer (Lepidoptera, Nymphalidae

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    Hélcio R. Gil-Santana

    2006-09-01

    Full Text Available Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera, Eulophidae is recorded as parasitoid of Dione juno juno (Cramer, 1779 (Lepidoptera, Nymphalidae in the State of Rio de Janeiro, Brazil.Palmistichus elaeisis Delvare & LaSalle, 1993 (Hymenoptera, Eulophidae é registrado como parasitóide de Dione juno juno (Cramer, 1779 (Lepidoptera, Nymphalidae, no estado do Rio de Janeiro, Brasil.

  18. Single locus complementary sex determination in Hymenoptera: an "unintelligent" design?

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    Driessen Gerard

    2006-01-01

    Full Text Available Abstract The haplodiploid sex determining mechanism in Hymenoptera (males are haploid, females are diploid has played an important role in the evolution of this insect order. In Hymenoptera sex is usually determined by a single locus, heterozygotes are female and hemizygotes are male. Under inbreeding, homozygous diploid and sterile males occur which form a genetic burden for a population. We review life history and genetical traits that may overcome the disadvantages of single locus complementary sex determination (sl-CSD. Behavioural adaptations to avoid matings between relatives include active dispersal from natal patches and mating preferences for non-relatives. In non-social species, temporal and spatial segregation of male and female offspring reduces the burden of sl-CSD. In social species, diploid males are produced at the expense of workers and female reproductives. In some social species, diploid males and diploid male producing queens are killed by workers. Diploid male production may have played a role in the evolution or maintenance of polygyny (multiple queens and polyandry (multiple mating. Some forms of thelytoky (parthenogenetic female production increase homozygosity and are therefore incompatible with sl-CSD. We discuss a number of hypothetical adaptations to sl-CSD which should be considered in future studies of this insect order.

  19. Avaliação do teor de umidade da madeira de Eucalyptus grandis por medidores elétricos resistivos. Evaluation of the Eucalyptus grandis lumber moisture content by resistancetype moisture meters.

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    João Eduardo Guarnetti dos SANTOS

    2006-01-01

    Full Text Available O presente estudo teve como objetivoverificar a precisão de dois tipos de medidoreselétricos de teor de umidade durante o processo desecagem da madeira de Eucalyptus grandis. Foramretiradas amostras representativas de 14 tábuas deEucalyptus grandis e secas em estufa elétrica a40 ºC de temperatura, até que o material atingisse10% de umidade. Durante a secagem foramdeterminados, periodicamente, o teor de umidadeatravés do método de massas correntes e deverificações simultâneas com um medidor elétricoportátil (EMM e com o sistema de controle de umsecador convencional (KCS. Os resultados mostraramque: (1 o sensor de umidade KCS pode substituiro método gravimétrico durante a secagem damadeira; (2 o medidor do teor de umidade EMMsubestima os reais teores de umidade durante asecagem da madeira e não é indicado parasubstituir o método gravimétrico de determinação de umidade.The aim of the study was to evaluate theprecision of two types of electric moisture metersduring the drying process of Eucalyptus grandisboards. Samples were obtained from 14 boards ofEucalyptus grandis and they were dried in electriclaboratory oven at 40 ºC of temperature, until thewood achieve 10% of moisture content. During thedrying, the moisture content was determined bygravimetric method and simultaneous checks by anelectric moisture meter (EMM and by kilncontrol system (KCS. The results showed that:(1 the KCS can replace the gravimetric methodduring the wood drying; (2 the EMMunderestimate the real moisture content during thedrying of boards and it is not indicate as substituteof the gravimetric method.

  20. A New Species of Vespula, and First Record of Vespa crabro L. (Hymenoptera:Vespidae) from Guatemala, Central America

    Science.gov (United States)

    Vespula akrei Landolt sp. nov. (Hymenoptera:Vespidae; Vespinae) is described from Guatemala. The first record of Vespa crabro L. (Hymenoptera:Vespidae:Vespinae) in Guatemala is given, and Vespula Inexspectata Eck (1994) from Mexico is re-described. We place Vespula akrei sp. nov. in the Vespula vulg...

  1. First occurrence of Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) preying on defoliating caterpillars of oil palm in the state of Para, Brazil; Primeira ocorrencia de Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) predando lagartas desfolhadoras do dendezeiro no estado do Para, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Rafael C.; Lemos, Walkymario P.; Muller, Antonio A. [EMBRAPA Amazonia Oriental, Belem, PA (Brazil). Lab. de Entomologia], e-mail: rafaufra@yahoo.com.br, e-mail: wplemos@cpatu.embrapa.br; Muller, Antonio A. [Embrapa Amazonia Oriental, Belem, PA (Brazil). Lab. de Entomologia; Bernardino, Aline S.; Buecke, Joel [Grupo Agropalma S/A., Tailandia, PA (Brazil)

    2010-01-15

    The oil palm Elaeis guineensis is usually attacked by pests, particularly, defoliating caterpillars. Between 2004 and 2006 a stinkbug predator (Asopinae) was registered preying on caterpillars of Brassolis sophorae L., Opsiphanes invirae Hubner (Lepidoptera: Nymphalidae) and Sibine spp. (Lepidoptera: Limacodidae), reducing their populations in commercial oil palm plantations in the State of Para, Brazil. Specimens of the natural enemy were collected, mounted, and identified as Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae), corresponding to the first report of the occurrence of this stinkbug attacking defoliating caterpillars of oil palm in Brazil. (author)

  2. Spatial Distribution of Adult Anthonomus grandis Boheman (Coleoptera: Curculionidae) and Damage to Cotton Flower Buds Due to Feeding and Oviposition.

    Science.gov (United States)

    Grigolli, J F J; Souza, L A; Fernandes, M G; Busoli, A C

    2017-08-01

    The cotton boll weevil Anthonomus grandis Boheman (Coleoptera: Curculionidae) is the main pest in cotton crop around the world, directly affecting cotton production. In order to establish a sequential sampling plan, it is crucial to understand the spatial distribution of the pest population and the damage it causes to the crop through the different developmental stages of cotton plants. Therefore, this study aimed to investigate the spatial distribution of adults in the cultivation area and their oviposition and feeding behavior throughout the development of the cotton plants. The experiment was conducted in Maracaju, Mato Grosso do Sul, Brazil, in the 2012/2013 and 2013/2014 growing seasons, in an area of 10,000 m 2 , planted with the cotton cultivar FM 993. The experimental area was divided into 100 plots of 100 m 2 (10 × 10 m) each, and five plants per plot were sampled weekly throughout the crop cycle. The number of flower buds with feeding and oviposition punctures and of adult A. grandis was recorded throughout the crop cycle in five plants per plot. After determining the aggregation indices (variance/mean ratio, Morisita's index, exponent k of the negative binomial distribution, and Green's coefficient) and adjusting the frequencies observed in the field to the distribution of frequencies (Poisson, negative binomial, and positive binomial) using the chi-squared test, it was observed that flower buds with punctures derived from feeding, oviposition, and feeding + oviposition showed an aggregated distribution in the cultivation area until 85 days after emergence and a random distribution after this stage. The adults of A. grandis presented a random distribution in the cultivation area.

  3. Selection of Reference Genes for qRT-PCR Analysis of Gene Expression in Stipa grandis during Environmental Stresses.

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    Dongli Wan

    Full Text Available Stipa grandis P. Smirn. is a dominant plant species in the typical steppe of the Xilingole Plateau of Inner Mongolia. Selection of suitable reference genes for the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR is important for gene expression analysis and research into the molecular mechanisms underlying the stress responses of S. grandis. In the present study, 15 candidate reference genes (EF1 beta, ACT, GAPDH, SamDC, CUL4, CAP, SNF2, SKIP1, SKIP5, SKIP11, UBC2, UBC15, UBC17, UCH, and HERC2 were evaluated for their stability as potential reference genes for qRT-PCR under different stresses. Four algorithms were used: GeNorm, NormFinder, BestKeeper, and RefFinder. The results showed that the most stable reference genes were different under different stress conditions: EF1beta and UBC15 during drought and salt stresses; ACT and GAPDH under heat stress; SKIP5 and UBC17 under cold stress; UBC15 and HERC2 under high pH stress; UBC2 and UBC15 under wounding stress; EF1beta and UBC17 under jasmonic acid treatment; UBC15 and CUL4 under abscisic acid treatment; and HERC2 and UBC17 under salicylic acid treatment. EF1beta and HERC2 were the most suitable genes for the global analysis of all samples. Furthermore, six target genes, SgPOD, SgPAL, SgLEA, SgLOX, SgHSP90 and SgPR1, were selected to validate the most and least stable reference genes under different treatments. Our results provide guidelines for reference gene selection for more accurate qRT-PCR quantification and will promote studies of gene expression in S. grandis subjected to environmental stress.

  4. Effect of soil water availability on gas exchange in young trees of Eucalyptus grandis W. Hill ex Maiden

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    María Sara Mejía de Tafur

    2017-10-01

    Full Text Available Two outdoor trials were conducted in the department of Valle del Cauca, Colombia, in contrasting climatic conditions, to evaluate the effect of soil water availability on the gas exchange of four elite genotypes of Eucalyptus grandis as follows: 28-3, 18-3, 24A-5 and 19-1, respectively. One trial was conducted on the campus of the Universidad Nacional de Colombia located in Palmira, at 994 m.a.s.l. with an average temperature of 23.5°C (maximum of 31°C, minimum of 18°C, and 76% relative humidity (RH. The second trial was conducted at the nursery of Carton de Colombia S.A., located in Restrepo, at 1450 m.a.s.l. with an average temperature 18°C (maximum of 26°C, minimum of 12°C and 80% RH. A split-plot design with four replicates was used. Treatments were field capacity (FC, ½ FC, ¼ FC, and soil waterlogging. At both locations, pot surfaces were covered with plastic sheeting to prevent the entrance of rainfall. Significant differences between water regimes, genotypes and locations occurred in photosynthesis rate, internal CO2 of the substomatal cavity (Ci, stomatal conductance, and transpiration rate, indicating that E. grandis has physiological defense mechanisms to drought stress such as stomata closure, evidenced by a decreasing in photosynthesis rate, stomatal conductance, and transpiration rate. A differential response was also observed between genotypes depending on the environment, indicating a genotype x environment interaction in terms of plant physiological response. Eucalyptus grandis appears to save water under water stress conditions by a decreasing in photosynthesis rate. Excess water is more limiting than water deficit.

  5. Effects of an inducible aiiA gene on disease resistance in Eucalyptus urophylla × Eucalyptus grandis.

    Science.gov (United States)

    Ouyang, L J; Li, L M

    2016-08-01

    N-acyl-homoserine lactones (AHLs) are metabolites of mostly gram-negative bacteria and are critical signaling molecules in bacterial quorum-sensing systems. At threshold concentrations, AHLs can activate the expression of pathogenic genes and induce diseases. Therefore, reducing AHL concentrations is a key point of disease control in plants. AHL-lactonase, which is expressed by aiiA, is widespread in Bacillus sp and can hydrolyze AHLs. In the present study, we cloned aiiA from Bacillus subtilis by PCR. A plant expression vector of aiiA was constructed and name Pcam-PPP3-aiiA, in which expression of aiiA was controlled by the pathogen-inducible plant promoter PPP3. The recombinant plasmid was transferred into Eucalyptus × urophylla × E. grandis by an Agrobacterium-mediated transformation. PCR and Southern blotting showed that aiiA was successfully integrated into the E. urophylla × E. grandis genome and its expression was induced by Ralstonia solanacearum 12 h after inoculation, as shown by reverse transcription-PCR. The transcription efficacy of aiiA increased 43.88-, 30.65-, and 18.95-fold after inoculation with R. solanacearum, Erwinia carotovora ssp. zeae (Sabet) and Cylindrocladium quinqueseptatum, respectively as shown by RT-real-time PCR. Transgenic E.urophylla × E.grandis expressing the AIIA protein exhibited significantly enhanced disease resistance compared to non-transgenic plants by delaying the onset of wilting and reducing the disease index.

  6. Traits and trade-offs in whole-tree hydraulic architecture along the vertical axis of Eucalyptus grandis.

    Science.gov (United States)

    Pfautsch, Sebastian; Aspinwall, Michael J; Drake, John E; Chacon-Doria, Larissa; Langelaan, Rob J A; Tissue, David T; Tjoelker, Mark G; Lens, Frederic

    2018-01-25

    Sapwood traits like vessel diameter and intervessel pit characteristics play key roles in maintaining hydraulic integrity of trees. Surprisingly little is known about how sapwood traits covary with tree height and how such trait-based variation could affect the efficiency of water transport in tall trees. This study presents a detailed analysis of structural and functional traits along the vertical axes of tall Eucalyptus grandis trees. To assess a wide range of anatomical and physiological traits, light and electron microscopy was used, as well as field measurements of tree architecture, water use, stem water potential and leaf area distribution. Strong apical dominance of water transport resulted in increased volumetric water supply per unit leaf area with tree height. This was realized by continued narrowing (from 250 to 20 µm) and an exponential increase in frequency (from 600 to 13 000 cm-2) of vessels towards the apex. The widest vessels were detected at least 4 m above the stem base, where they were associated with the thickest intervessel pit membranes. In addition, this study established the lower limit of pit membrane thickness in tall E. grandis at ~375 nm. This minimum thickness was maintained over a large distance in the upper stem, where vessel diameters continued to narrow. The analyses of xylem ultrastructure revealed complex, synchronized trait covariation and trade-offs with increasing height in E. grandis. Anatomical traits related to xylem vessels and those related to architecture of pit membranes were found to increase efficiency and apical dominance of water transport. This study underlines the importance of studying tree hydraulic functioning at organismal scale. Results presented here will improve understanding height-dependent structure-function patterns in tall trees. © The Author(s) 2018. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  7. Blood Sugar Lowering Effect of Coccinia grandis (L. J. Voigt: Path for a New Drug for Diabetes Mellitus

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    M. A. A. K. Munasinghe

    2011-01-01

    Full Text Available Background. Role of herbs in the management and control of diabetes has emerged fast over the years. We assessed the efficacy of Coccinia grandis (locally known as Ken, Kovakka leaves as a hypoglycemic agent. Methods. Double-blind phase I clinical trial was conducted at the general hospital and a private hospital in Matara in August 2009. All the participants were given a common meal for dinner, and they maintained a 10-hour fasting period. Sixty-one healthy volunteers were given a meal containing 20 g of leaves of Coccinia grandis which was mixed with a measured amount of scraped coconut and table salt for breakfast, and other 61 were given the placebo meal which also contained scraped coconut and salt. Glucose tolerance test was performed blindly for the two groups. Mixed factorial design analysis of variance and student's t-test were applied. Results. Overall blood sugar levels of the experimental group were also significantly lower than those of the control group (F(1,117 5.56, <0.05. Increase in the blood sugar levels from fasting to one hour (F(1,117 6.77, <0.05 and two hours (F(1,117 5.28, <0.05 postprandially was statistically significant for participants who were in the control group than those of in the experimental group. The mean difference of postprandial blood sugar levels (mg/dL after one hour (20.2, 95% confidence interval, 4.81 to 35.5 and two hours (11.46, 95% confidence interval; 1.03 to 21.9 was statistically significant between the two groups. Conclusions. Coccinia grandis has a blood sugar lowering effect. However further studies are needed to validate our findings.

  8. Eradication of the cotton boll weevil (Anthonomus grandis) in the United States - A successful multi-regional approach

    International Nuclear Information System (INIS)

    Cunningham, Gary L.; Grefenstette, William J.

    2000-01-01

    The cotton boll weevil, Anthonomus grandis Boheman, is believed to have entered the US from Mexico and was first detected in South Texas in 1892. Since that time, the pest has spread throughout most of the nation's cotton-producing areas and has become the industry's number one nemesis. More than US$13 billion in economic losses have occurred since its introduction, with recent annual expenditures of more than US$300 million for control costs alone. Although the weevil has been eradicated from over 4 million acres, its presence in non-programme areas continues to dictate production practices within the mid-south, Texas and Oklahoma

  9. PRODUCTIVITY AND COSTS OF PROCESSOR WORKING IN STANDS OF Eucalyptus grandis Hill ex Maiden

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    Bernardo Carlos Tarnowski

    2009-09-01

    Full Text Available In the present work a time study was conducted with the objective of adjusting equations to estimate the time of activities, productivity, operational costs and the production of the processor used in a harvest operation of stands of Eucalyptus grandis in plain topography in the state of Bahia, Brazil. The operational cycle of the processor consisted of the time spent to process a tree, and was divided in to stages, which were assessed using the methotodology of single activity times. The sampling unit was the operational cycle of the machine. The statistical analysis was based on regression analysis considering the selection procedure “stepwise”. With the adjusted equations it was possible to estimate the productivity of the machine taking into account the of tree diameter. Considering an operational efficiency of 70 % under the circumstances of the study, the productivity of the processor was 25,8 m3 cc/h, the operational costs 47,90 US$/h and the production costs 1,86 US$/m3 cc. On the basis of the obtained results it can be concluded that the time of tree processing has varied directly according to the diameter increase diameter; the preparation time, contrary to the processing time, only shows a weak correlation with tree diameter; productivity of the processor is directly proportional to tree diameter, when expressed in volume and inversely proportional when expressed in tree number; the costs per cubic meter of wood processed varies inversely with of increased diameter; from the operational costs, fixed costs had the highest proportion followed by the variable costs, administrative costs and costs for manpower; the production costs of the processor decreased exponentially with increasing tree diameter.

  10. Influence of Two Acyclic Homoterpenes (Tetranorterpenes) on the Foraging Behavior of Anthonomus grandis Boh.

    Science.gov (United States)

    Magalhães, D M; Borges, M; Laumann, R A; Woodcock, C M; Pickett, J A; Birkett, M A; Blassioli-Moraes, Maria Carolina

    2016-04-01

    Previous studies have shown that the boll weevil, Anthonomus grandis, is attracted to constitutive and conspecific herbivore-induced cotton volatiles, preferring the blend emitted by cotton at the reproductive over the vegetative stage. Moreover, this preference was paralleled by the release of the acyclic homoterpenes (tetranorterpenes) (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT) in Delta Opal cotton being higher at the vegetative than at the reproductive stage. Here, we evaluated whether this difference in release of acyclic homoterpenes also occurred in other cotton varieties, and if boll weevils could recognize these compounds as indicators of a specific cotton phenological stage. Results showed that cotton genotypes CNPA TB-90, BRS-293 and Delta Opal all produced higher levels of DMNT and TMTT at the vegetative stage than at the reproductive stage and that these homoterpenes allowed for principal component analysis separation of volatiles produced by the two phenological stages. Electroantennograms confirmed boll weevil antennal responses to DMNT and TMTT. Behavioral assays, using Y-tube olfactometers, showed that adding synthetic homoterpenes to reproductive cotton volatiles (mimicking cotton at the vegetative stage in terms of homoterpene levels) resulted in reduced attraction to boll weevils compared to that to unmodified reproductive cotton. Weevils showed no preference when given a choice between plants at the vegetative stage and the vegetative stage-mimicked plant. Altogether, the results show that DMNT and TMTT are used by boll weevils to distinguish between cotton phenological stages.

  11. Proteolytic processing of the vitellogenin precursor in the boll weevil, Anthonomus grandis.

    Science.gov (United States)

    Heilmann, L J; Trewitt, P M; Kumaran, A K

    1993-01-01

    The soluble proteins of the eggs of the coleopteran insect Anthonomus grandis Boheman, the cotton boll weevil, consist almost entirely of two vitellin types with M(r)s of 160,000 and 47,000. We sequenced their N-terminal ends and one internal cyanogen bromide fragment of the large vitellin and compared these sequences with the deduced amino acid sequence from the vitellogenin gene. The results suggest that both the boll weevil vitellin proteins are products of the proteolytic cleavage of a single precursor protein. The smaller 47,000 M(r) vitellin protein is derived from the N-terminal portion of the precursor adjacent to an 18 amino acid signal peptide. The cleavage site between the large and small vitellins at amino acid 362 is adjacent to a pentapeptide sequence containing two pairs of arginine residues. Comparison of the boll weevil sequences with limited known sequences from the single 180,000 M(r) honey bee protein show that the honey bee vitellin N-terminal exhibits sequence homology to the N-terminal of the 47,000 M(r) boll weevil vitellin. Treatment of the vitellins with an N-glycosidase results in a decrease in molecular weight of both proteins, from 47,000 to 39,000 and from 160,000 to 145,000, indicating that about 10-15% of the molecular weight of each vitellin consists of N-linked carbohydrate. The molecular weight of the deglycosylated large vitellin is smaller than that predicted from the gene sequence, indicating possible further proteolytic processing at the C-terminal of that protein.

  12. Combined Effects of Deepwater Horizon Crude Oil and Environmental Stressors on Fundulus grandis Embryos.

    Science.gov (United States)

    Rodgers, Maria L; Jones, Elizabeth R; Klinkhamer, Christopher; Mahapatra, Cecon T; Serafin, Jennifer; Bosker, Thijs; Perkins, Christopher; Griffitt, Robert J; De Guise, Sylvain; Sepúlveda, Maria S

    2018-04-16

    In this study, we examined how sensitivity to oil changes in combination with environmental stressors in Fundulus grandis embryos. We exposed embryos (< 24 hours post fertilization) to a range of High Energy Water Accommodated Fraction (HEWAF) concentrations (0-50 ppb total polycyclic aromatic hydrocarbons) made from Macondo oil in conjunction with various environmental conditions (temperature: 20, 30°C; salinity: 3, 7, 30 practical salinity units (PSU); DO: 2, 6 mg/L). Endpoints included mortality, hatching rates, and expression of cytochrome p450 1a and 1c (cyp1a, cyp1c) in hatched larvae. There was 100% mortality for all fish under the 2 ppm dissolved oxygen (DO) regimes. For the 6 mg/L DO treatments, mortality and mean lethal time were generally higher in the 30°C treatments versus the 20°C treatments. Oil increased mortality in fish exposed to the highest concentration in the 20-3-6 (°C-PSU-mg/L), 25-7-6, and 30-30-6 conditions. Hatching was driven by environmental conditions, with oil exposure only having a significant impact on hatching in the 25-7-6 and 30-30-6 groups at the highest HEWAF exposure. Expression of cyp1a was upregulated in most treatment groups versus the controls, with cyp1c expression exhibiting a similar pattern. These data suggest interactive effects among temperature, salinity, and PAHs, highlighting a need to further assess the effects of oil exposure under various environmental conditions. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  13. Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis fruits in Vietnam.

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    Ivana Puglisi

    Full Text Available Two distinct Phytophthora taxa were found to be associated with brown rot of pomelo (Citrus grandis, a new disease of this ancestral Citrus species, in the Vinh Long province, Mekong River Delta area, southern Vietnam. On the basis of morphological characters and using the ITS1-5.8S-ITS2 region of the rDNA and the cytochrome oxidase subunit 1 (COI as barcode genes, one of the two taxa was provisionally named as Phytophthora sp. prodigiosa, being closely related to but distinct from P. insolita, a species in Phytophthora Clade 9, while the other one, was closely related to but distinct from the Clade 2 species P. meadii and was informally designated as Phytophthora sp. mekongensis. Isolates of P. sp. prodigiosa and P. sp. mekongensis were also obtained from necrotic fibrous roots of Volkamer lemon (C. volkameriana rootstocks grafted with 'King' mandarin (Citrus nobilis and from trees of pomelo, respectively, in other provinces of the Mekong River Delta, indicating a widespread occurrence of both Phytophthora species in this citrus-growing area. Koch's postulates were fulfilled via pathogenicity tests on fruits of various Citrus species, including pomelo, grapefruit (Citrus x paradisi, sweet orange (Citrus x sinensis and bergamot (Citrus x bergamia as well as on the rootstock of 2-year-old trees of pomelo and sweet orange on 'Carrizo' citrange (C. sinensis 'Washington Navel' x Poncirus trifoliata. This is the first report of a Phytophthora species from Clade 2 other than P. citricola and P. citrophthora as causal agent of fruit brown rot of Citrus worldwide and the first report of P. insolita complex in Vietnam. Results indicate that likely Vietnam is still an unexplored reservoir of Phytophthora diversity.

  14. Nutrient concentration age dynamics of teak (Tectona grandis L.f.) plantations in Central America

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez-Moya, J.; Murillo, R.; Portuguez, E.; Fallas, J. L.; Rios, V.; Kottman, F.; Verjans, J. M.; Mata, R.; Alvarado, A.

    2013-05-01

    Aim of study. Appropriate knowledge regarding teak (Tectona grandis L.f.) nutrition is required for a better management of the plantations to attain high productivity and sustainability. This study aims to answer the following questions: How can it be determined if a teak tree suffers a nutrient deficiency before it shows symptoms? Are nutrient concentration decreases in older trees associated with age-related declines in forest productivity? Area of study. Costa Rica and Panama. Material and Methods. Nutrient concentration in different tree tissues (bole, bark, branches and foliage) were measured at different ages using false-time-series in 28 teak plantations Research highlights. Foliar N concentration decreases from 2.28 in year 1 to 1.76% in year 19. Foliar Mg concentration increases from 0.23 in year 1 to 0.34% in year 19. The foliar concentrations of the other nutrients are assumed to be constant with tree age: 1.33% Ca, 0.88% K, 0.16% P, 0.12% S, 130 mg kg{sup -}1 Fe, 43 mg kg{sup -}1 Mn, 11 mg kg{sup -}1 Cu, 32 mg kg{sup -}1 Zn and 20 mg kg{sup -}1 B. The nutrient concentration values showed can be taken as a reference to evaluate the nutritional status of similar teak plantations in the region. The concentrations of K, Mg and N could be associated with declines in teak plantation productivity as the plantation becomes older. Whether age-related changes in nutrient concentrations are a cause or a consequence of age-related declines in productivity is an issue for future research with the aim of achieving higher growth rates throughout the rotation period. (Author) 35 refs.

  15. Development and evaluation of teak (Tectona grandis L.f. taper equations in northern Thailand

    Directory of Open Access Journals (Sweden)

    Andrew J. Warner

    2016-09-01

    Full Text Available Taper refers to the general decrease in the regular outline of a solid body from its base to its tip. Taper models are used to estimate the volume and value of wood products from harvesting trees. Teak (Tectona grandis L.f. is highly valued as one of the world's most preferred timbers and a teak taper equation is required to inform optimal harvesting strategies given the limited plantation resource available in Thailand. Teak taper equations were developed and evaluated based on 331 sample trees collected in 2014 from eight plantations in northern Thailand aged from 10 to 46 yr using two taper model formulations—the Kozak variable-exponent taper model and the Goodwin cubic polynomial model comprising hyperbolic and parabolic terms. Variants based on both model types were fitted using nonlinear regression analysis with diameter at breast height, total tree height and height of girth measurement as the independent variables to estimate diameter underbark at the nominated height. Goodness-of-fit and leave-one-out cross validation with lack-of-fit statistical testing combined with extensive graphical analysis of residuals were used to select the best model. A Goodwin model variant (named FIO-teak1 as the first plantation teak taper model known to be published in Thailand provided the best estimates of volume and diameter underbark. A simple case study confirmed that FIO-teak1 in combination with the Farm Forestry Toolbox software package could assist teak plantation managers in decision making associated with optimizing log grade value based on standing tree inventory data.

  16. Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam.

    Science.gov (United States)

    Puglisi, Ivana; De Patrizio, Alessandro; Schena, Leonardo; Jung, Thomas; Evoli, Maria; Pane, Antonella; Van Hoa, Nguyen; Van Tri, Mai; Wright, Sandra; Ramstedt, Mauritz; Olsson, Christer; Faedda, Roberto; Magnano di San Lio, Gaetano; Cacciola, Santa Olga

    2017-01-01

    Two distinct Phytophthora taxa were found to be associated with brown rot of pomelo (Citrus grandis), a new disease of this ancestral Citrus species, in the Vinh Long province, Mekong River Delta area, southern Vietnam. On the basis of morphological characters and using the ITS1-5.8S-ITS2 region of the rDNA and the cytochrome oxidase subunit 1 (COI) as barcode genes, one of the two taxa was provisionally named as Phytophthora sp. prodigiosa, being closely related to but distinct from P. insolita, a species in Phytophthora Clade 9, while the other one, was closely related to but distinct from the Clade 2 species P. meadii and was informally designated as Phytophthora sp. mekongensis. Isolates of P. sp. prodigiosa and P. sp. mekongensis were also obtained from necrotic fibrous roots of Volkamer lemon (C. volkameriana) rootstocks grafted with 'King' mandarin (Citrus nobilis) and from trees of pomelo, respectively, in other provinces of the Mekong River Delta, indicating a widespread occurrence of both Phytophthora species in this citrus-growing area. Koch's postulates were fulfilled via pathogenicity tests on fruits of various Citrus species, including pomelo, grapefruit (Citrus x paradisi), sweet orange (Citrus x sinensis) and bergamot (Citrus x bergamia) as well as on the rootstock of 2-year-old trees of pomelo and sweet orange on 'Carrizo' citrange (C. sinensis 'Washington Navel' x Poncirus trifoliata). This is the first report of a Phytophthora species from Clade 2 other than P. citricola and P. citrophthora as causal agent of fruit brown rot of Citrus worldwide and the first report of P. insolita complex in Vietnam. Results indicate that likely Vietnam is still an unexplored reservoir of Phytophthora diversity.

  17. Sulfur-Mediated-Alleviation of Aluminum-Toxicity in Citrus grandis Seedlings

    Directory of Open Access Journals (Sweden)

    Peng Guo

    2017-12-01

    Full Text Available Limited data are available on the sulfur (S-mediated-alleviation of aluminum (Al-toxicity in higher plants. Citrus grandis seedlings were irrigated for 18 weeks with 0.5 mM MgSO4 or 0.5 mM MgSO4 + 0.5 mM Na2SO4, and 0 (−Al or 1 mM AlCl3·6H2O (+Al, Al-toxicity. Under Al-toxicity, S decreased the level of Al in leaves; increased the relative water content (RWC of roots and leaves, the contents of phosphorus (P, calcium (Ca and magnesium (Mg per plant, the dry weights (DW of roots and shoots, the ratios of root DW/shoot DW, and the Al-induced secretion of citrate from root; and alleviated the Al-induced inhibition of photosynthesis via mitigating the Al-induced decrease of electron transport capacity resulting from the impaired photosynthetic electron transport chain. In addition to decreasing the Al-stimulated H2O2 production, the S-induced upregulation of both S metabolism-related enzymes and antioxidant enzymes also contributed to the S-mediated-alleviation of oxidative damage in Al-treated roots and leaves. Decreased transport of Al from roots to shoots and relatively little accumulation of Al in leaves, and increased leaf and root RWC and P, Ca, and Mg contents per plant might also play a role in the S-mediated-alleviation of Al-toxicity.

  18. Effects of impregnation of Eucalyptus grandis wood with fire-retardant salt on the production and quality of its charcoal; Efeito da impregnacao da madeira de Eucalyptus grandis com sais ignifugos na producao e na qualidade do carvao

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Ana Marcia Macedo Ladeira; Vital, Benedito Rocha; Gomide, Jose Livio; Della Lucia, Ricardo Marius; Leite, Helio Garcia [Vicosa Univ., MG (Brazil). Dept. de Engenharia Florestal

    1998-12-31

    The present work was carried out to investigate the effects of impregnating Eucalyptus grandis wood fire retardant salts on the production and quality of its charcoal. The wood used came from a commercial stand of 72-month old Eucalyptus grandis planted at an initial 3.0 x 2.0 m spacing, established in Vicosa, Minas Gerais, Brazil. This wood was transformed in chips, treated with some fire-retardant salts and carbonized. results were evaluated through multivariate analysis. The difference among the 34 treatments, evaluated on the wood characteristics, based on group analysis, and using the distance D{sup 2} of Mahalanobis and the Tocher method, showed distinct group and subgroup formations. Based on technological data, treatment with ammonium sulphate at 15% concentration was classified as being the most promising one. The use of canonic variables analysis showed that the treatment with ammonium sulphate at a concentration of 5% was the best, and was given preference because of its lower sulphur content. (author) 12 refs., 1 fig., 7 tabs.

  19. KEANEKARAGAMAN HYMENOPTERA PARASITOID PADA PERKEBUNAN KELAPA SAWIT PTPN VIII CINDALI, BOGOR

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    Ichsan Luqmana Indra Putra

    2016-09-01

    Full Text Available Diversity of parasitic Hymenoptera in PTPN VIII oil palm plantation Cindali, Bogor. One group of important natural enemies in oil palm plantation is parasitic Hymenoptera. The purpose of this research is to know the diversity and fluctuation of parasitic Hymenoptera PTPN VIII Cindali oil palm plantation. This research was conducted in 6 blocks of oil palm in September 2014 – June 2015. There were 5 plots in every observation blocks 39.2 x 39.2 m in size, and used direct and indirect method. Direct method done by 5 plants in every plots was taken randomized to observed and taken the herbivore insects to reared until the parasitic Hymenoptera came out. Observation of cover crops conducted by 3 subplots determined diagonally in every plots 9.8 x 9.8 m in size and herbivore insects was observed and collected. Indirect methods used sweep net and yellow pan trap. The result of this research, 26 parasitic Hymenoptera families was found, with the Braconidae was the most morphospecies found and the most individual amount was Scelionidae. The abundance of parasitoid in every month fluctuated.

  20. The Community of Hymenoptera Parasitizing Necrophagous Diptera in an Urban Biotope

    Science.gov (United States)

    Frederickx, Christine; Dekeirsschieter, Jessica; Verheggen, François J.; Haubruge, Eric

    2013-01-01

    Most reports published in the field of forensic entomology are focused on Diptera and neglect the Hymenoptera community. However, Hymenoptera are part of the entomofaunal colonization of a dead body. The use of Hymenoptera parasitoids in forensic entomology can be relevant to evaluate the time of death. Hymenoptera parasitoids of the larvae and pupae of flies may play an important role in the estimation of the post-mortem period because their time of attack is often restricted to a small, well-defined window of time in the development of the host insect. However, these parasitoids can interfere with the developmental times of colonizing Diptera, and therefore a better understanding of their ecology is needed. The work reported here monitored the presence of adult Hymenoptera parasitoids on decaying pig carcasses in an urban biotope during the summer season (from May to September). Six families and six species of parasitoids were recorded in the field: Aspilota fuscicornis Haliday (Braconidae), Alysia manducator Panzer, Nasonia vitripennis Walker (Pteromalidae), Tachinaephagus zealandicus Ashmead (Encyrtidae), Trichopria sp. (Diapriidae), and Figites sp. (Figitidae). In the laboratory, five species emerged from pupae collected in the field: Trichopria sp., Figites sp., A. manducator, N. vitripennis, and T. zealandicus. These five species colonize a broad spectrum of Diptera hosts, including those species associated with decomposing carcasses, namely those from the families Calliphoridae, Muscidae, Fanniidae, and Sarcophagidae. PMID:23895458

  1. Replication of Chilo iridescent virus in the cotton boll weevil, Anthonomus grandis, and development of an infectivity assay.

    Science.gov (United States)

    Henderson, C W; Johnson, C L; Lodhi, S A; Bilimoria, S L

    2001-01-01

    The boll weevil, Anthonomus grandis Boheman, is a devastating pest of cotton. Chemical pesticides are problematic due to relative lack of target specificity and resistance. Microbial pesticides may provide viable alternatives because of their narrow host range. Chilo iridescent virus (CIV) is the type species for genus Iridovirus, family Iridoviridae: large, icosahedral cytoplasmic viruses containing a double-stranded DNA genome. Earlier work suggested that CIV replicated in the boll weevil; however, efficiency or production of infectious virus was not established. We showed that CIV undergoes a productive cycle in A. grandis. CIV DNA levels in boll weevil pupae increased significantly from 0 to 3 days post infection. Moreover, virogenic stromata and complete virus particles were observed in the cytoplasm by 7 days. An endpoint dilution assay using viral DNA replication as indicator suggested a 10(5)-fold increase in infectious virus titer over 7 days. This is the first such demonstration in larval infections with genus Iridovirus. Our study establishes that CIV undergoes a productive cycle in the boll weevil and provides an important and useful model system for replication at the organismal level. These results have important implications for the potential of CIV and its components in boll weevil control.

  2. Amenizante Orgânico e Eucalyptus grandis para Fitoestabilização de Solo Contaminado com Cobre

    Directory of Open Access Journals (Sweden)

    Rudinei De Marco

    2017-04-01

    Full Text Available RESUMO Este trabalho avaliou a utilização de turfa como amenizante e Eucalyptus grandis como técnica de fitoestabilização para remediar solo contaminado com cobre. O trabalho foi conduzido sob delineamento inteiramente casualizado em arranjo fatorial (2 × 6, sendo sem e com adição de turfa (200 mL L-1 de solo e seis doses de cobre adicionadas ao solo (0 mg kg-1, 60 mg kg-1, 120 mg kg-1, 180 mg kg-1, 240 mg kg-1, 300 mg kg-1 de solo, com seis repetições. Avaliaram-se a altura das mudas, o diâmetro do colo, a massa seca radicular e aérea, a área superficial específica, os teores e a quantidade acumulada de cobre no sistema radicular e na parte aérea e o índice de translocação. A adição de turfa possibilitou efeito amenizante da contaminação por cobre e favoreceu o crescimento das mudas. As mudas de Eucalyptus grandis apresentam elevado acúmulo de cobre nas raízes, o que permite indicá-la como promissora para fins de fitoestabilização de solos contaminados com cobre.

  3. Development of seedless fruits mutants in citrus including tangerine (C. reticulata) and pummelo (C. grandis) through induced mutations and biotechnology

    Energy Technology Data Exchange (ETDEWEB)

    Somsri, S. [Horticulture Research Institute, Department of Agriculture (DOA), Chatuchak, Bangkok (Thailand); Jompook, P. [Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Chatuchak, Bangkok (Thailand); Kanhom, P. [Phare Horticultural Research Center, Muang, Phare (Thailand); Thayamanont, P.; Meecharoen, S. [Pichit Horticultural Research Center, Muang, Pichit (Thailand); Kumcha, U. [Srisaket Horticultural Research Center, Muang, Srisaket (Thailand)

    2009-05-15

    The development of seedless fruit mutants in citrus, including Tangerine (C. reticulata) and Pummelo (C. grandis), through induced mutation and biotechnology was studied at the Gamma Irradiation Service and Nuclear Technology Center, Pichit and Phare Horticultural Research Center for 4 years (August 2000 to September 2004). The results showed successful induction of mutants with gamma irradiation using both chronic and acute procedures for pot plants, scions and in vitro plantlets of tangerine (Citrus reticulata var. Shogun and Sai Nam Puaeng) and pummelo (Citrus grandis viz. Kao Thong Dee). MS medium with 2 mgL{sup -1} of BA was found to be the most suitable medium for shoot proliferation. The seedlings were sub-cultured at least 4 times, and then they were treated with acute and chronic irradiation. Shoot induction from M{sub 1}V{sub 0} to M{sub 1}V{sub 4} generation was performed in basic MS medium with 2 mgL{sup -1} added BA. Rooting was induced in the M{sub 1}V{sub 4} in halfstrength MS enriched with BA 2 mgL{sup -1}. Later, the shoots were excised and grafted on mature plants or the plantlets directly transferred in the field and later the fruits from mature trees were evaluated for seedlessness in M{sub 1}V{sub 4} at Pichit and Phare Horticultural Research Center. (author)

  4. In vivo antitussive activity of Coccinia grandis against irritant aerosol and sulfur dioxide-induced cough model in rodents

    Directory of Open Access Journals (Sweden)

    Shakti Prasad Pattanayak and Priyashree Sunita

    2009-12-01

    Full Text Available Coccinia grandis (Cucurbitaceae has extensively used to get relief from asthma and cough by the indigenous people of India. The antitussive effect of aerosols of two different concentrations (2.5%, 5% w/v of methanol extract of C. grandis fruits were tested by counting the numbers of coughs produced due to aerosols of citric acid, 10 min after exposing the male guinea pigs to aerosols of test solutions for 7 min. In another set of experiment methanol extract was investigated for its therapeutic efficacy on a cough model induced by sulfur dioxide gas in mice. The results showed significant reduction of cough number obtained in the presence of both concentrations of methanol extract as that of the prototype antitussive agent codeine phosphate. Also, methanol extract exhibited significant antitussive effect at 100, 200 and 400 mg/kg, per orally by inhibiting the cough by 20.57, 33.73 and 56.71% within 90 min of performing the experiment respectively.

  5. MICOBIOTA ASSOCIADA À MADEIRA SERRADA DE Eucalyptus grandis HILL EX MAIDEN DURANTE A SECAGEM AO AR LIVRE

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    Paulo Fernando Trugilho

    2006-02-01

    Full Text Available O objetivo principal deste trabalho foi o de acompanhar a secagem ao ar livre da madeira serrada de Eucalyptus grandis conduzida na região de Lavras, MG e identificar a micobiota associada à madeira em diversas fases da secagem. As tábuas foram cortadas de oito toras de 3,0 m de comprimento, oriundas de três árvores, com 27 anos de idade, plantadas em área experimental da UFLA. Dos resultados, pôde-se concluir que a secagem da madeira de Eucalyptus grandis ao ar livre, iniciada em janeiro, consumiu 158 dias até atingir umidade próxima a 12,5%. A curva de secagem foi representada por uma equação logarítmica com coeficiente de determinação (R2 igual a 98,3%. Os fungos causadores de bolores superficiais e de manchas tiveram maior ocorrência no início da secagem. As mais altas freqüências de fungos, verificadas no início da secagem, foram observadas para Penicillium spp. e Pestalotiopsis sp. O fungo Lentinus lepideus ocorreu com maior freqüência no final da secagem.

  6. The Eucalyptus grandis R2R3-MYB transcription factor family: evidence for woody growth-related evolution and function.

    Science.gov (United States)

    Soler, Marçal; Camargo, Eduardo Leal Oliveira; Carocha, Victor; Cassan-Wang, Hua; San Clemente, Hélène; Savelli, Bruno; Hefer, Charles A; Paiva, Jorge A Pinto; Myburg, Alexander A; Grima-Pettenati, Jacqueline

    2015-06-01

    The R2R3-MYB family, one of the largest transcription factor families in higher plants, controls a wide variety of plant-specific processes including, notably, phenylpropanoid metabolism and secondary cell wall formation. We performed a genome-wide analysis of this superfamily in Eucalyptus, one of the most planted hardwood trees world-wide. A total of 141 predicted R2R3-MYB sequences identified in the Eucalyptus grandis genome sequence were subjected to comparative phylogenetic analyses with Arabidopsis thaliana, Oryza sativa, Populus trichocarpa and Vitis vinifera. We analysed features such as gene structure, conserved motifs and genome location. Transcript abundance patterns were assessed by RNAseq and validated by high-throughput quantitative PCR. We found some R2R3-MYB subgroups with expanded membership in E. grandis, V. vinifera and P. trichocarpa, and others preferentially found in woody species, suggesting diversification of specific functions in woody plants. By contrast, subgroups containing key genes regulating lignin biosynthesis and secondary cell wall formation are more conserved across all of the species analysed. In Eucalyptus, R2R3-MYB tandem gene duplications seem to disproportionately affect woody-preferential and woody-expanded subgroups. Interestingly, some of the genes belonging to woody-preferential subgroups show higher expression in the cambial region, suggesting a putative role in the regulation of secondary growth. © 2014 The Authors New Phytologist © 2014 New Phytologist Trust.

  7. Genome-Wide Characterization and Expression Profiling of the AUXIN RESPONSE FACTOR (ARF) Gene Family in Eucalyptus grandis

    Science.gov (United States)

    Yu, Hong; Soler, Marçal; Mila, Isabelle; San Clemente, Hélène; Savelli, Bruno; Dunand, Christophe; Paiva, Jorge A. P.; Myburg, Alexander A.; Bouzayen, Mondher; Grima-Pettenati, Jacqueline; Cassan-Wang, Hua

    2014-01-01

    Auxin is a central hormone involved in a wide range of developmental processes including the specification of vascular stem cells. Auxin Response Factors (ARF) are important actors of the auxin signalling pathway, regulating the transcription of auxin-responsive genes through direct binding to their promoters. The recent availability of the Eucalyptus grandis genome sequence allowed us to examine the characteristics and evolutionary history of this gene family in a woody plant of high economic importance. With 17 members, the E. grandis ARF gene family is slightly contracted, as compared to those of most angiosperms studied hitherto, lacking traces of duplication events. In silico analysis of alternative transcripts and gene truncation suggested that these two mechanisms were preeminent in shaping the functional diversity of the ARF family in Eucalyptus. Comparative phylogenetic analyses with genomes of other taxonomic lineages revealed the presence of a new ARF clade found preferentially in woody and/or perennial plants. High-throughput expression profiling among different organs and tissues and in response to environmental cues highlighted genes expressed in vascular cambium and/or developing xylem, responding dynamically to various environmental stimuli. Finally, this study allowed identification of three ARF candidates potentially involved in the auxin-regulated transcriptional program underlying wood formation. PMID:25269088

  8. Microbiome analysis shows enrichment for specific bacteria in separate anatomical regions of the deep-sea carnivorous sponge Chondrocladia grandis.

    Science.gov (United States)

    Verhoeven, Joost T P; Kavanagh, Alana N; Dufour, Suzanne C

    2017-01-01

    The Cladorhizidae is a unique family of carnivorous marine sponges characterised by either the absence or reduction of the aquiferous system and by the presence of specialised structures to trap and digest mesoplanktonic prey. Previous studies have postulated a key role of host-associated bacteria in enabling carnivory in this family of sponges. In this study, we employed high-throughput Illumina-based sequencing to identify the bacterial community associated with four individuals of the deep-sea sponge Chondrocladia grandis sampled in the Gulf of Maine. By characterising the V6 through V8 region of the 16S rRNA gene, we compared the bacterial community composition and diversity in three distinct anatomical regions with predicted involvement in prey capture (sphere), support (axis) and benthic substrate attachment (root). A high abundance of Tenacibaculum, a known siderophore producing bacterial genus, was present in all anatomical regions and specimens. The abundance of Colwellia and Roseobacter was greater in sphere and axis samples, and bacteria from the hydrocarbon-degrading Robiginitomaculum genus were most abundant in the root. This first description of the bacterial community associated with C. grandis provides novel insights into the contribution of bacteria to the carnivorous lifestyle while laying foundations for future cladorhizid symbiosis studies. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  9. Genome-wide characterization and expression profiling of the AUXIN RESPONSE FACTOR (ARF gene family in Eucalyptus grandis.

    Directory of Open Access Journals (Sweden)

    Hong Yu

    Full Text Available Auxin is a central hormone involved in a wide range of developmental processes including the specification of vascular stem cells. Auxin Response Factors (ARF are important actors of the auxin signalling pathway, regulating the transcription of auxin-responsive genes through direct binding to their promoters. The recent availability of the Eucalyptus grandis genome sequence allowed us to examine the characteristics and evolutionary history of this gene family in a woody plant of high economic importance. With 17 members, the E. grandis ARF gene family is slightly contracted, as compared to those of most angiosperms studied hitherto, lacking traces of duplication events. In silico analysis of alternative transcripts and gene truncation suggested that these two mechanisms were preeminent in shaping the functional diversity of the ARF family in Eucalyptus. Comparative phylogenetic analyses with genomes of other taxonomic lineages revealed the presence of a new ARF clade found preferentially in woody and/or perennial plants. High-throughput expression profiling among different organs and tissues and in response to environmental cues highlighted genes expressed in vascular cambium and/or developing xylem, responding dynamically to various environmental stimuli. Finally, this study allowed identification of three ARF candidates potentially involved in the auxin-regulated transcriptional program underlying wood formation.

  10. On the identity of Melipona torrida Friese (Hymenoptera, Apidae

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    Gabriel A. R. Melo

    2013-09-01

    Full Text Available On the identity of Melipona torrida Friese (Hymenoptera, Apidae. Melipona marginata var. torrida Friese, 1916, described from three workers putatively collected in Costa Rica, never had its identity properly recognized. Since its original description, no additional specimens have ever been collected in Costa Rica. It is argued here that Melipona torrida was based on mislabeled specimens and corresponds to Melipona marginata obscurior Moure, 1971, a form known only from southern Brazil, Argentina and Paraguay. A lectotype is designated for Melipona torrida and notes on the type material of Melipona marginata obscurior are provided. Other known examples of species described from mislabeled specimens in Friese's Zur Bienenfauna von Costa Rica are discussed. It is pointed out that additional names proposed in this work, based on material from Costa Rica, might turn out to correspond to South American taxa. Also, the date of publication of this Friese's paper is discussed.

  11. Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae by fluorochromes

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    Jefferson de Brito Marthe

    2010-01-01

    Full Text Available Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32+2. Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P. cupira. The data obtained suggests that B chromosomes in P. helleri and P. cupira share a common origin.

  12. Integrating remote sensing and ancillary data for regional ecosystem assessment: eucalyptus grandis agro-system in kwazulu-Natal, South Africa

    CSIR Research Space (South Africa)

    Cho, Moses A

    2009-07-01

    Full Text Available The study demonstrates that the integration of remote sensing and in situ data could be important in providing more accurate estimates of E. grandis state in KwaZulu Natal, South Africa compared to remote sensing models. Such modelling effort would...

  13. Midgut GPI-anchored proteins with alkaline phosphatase activity from the cotton boll weevil (Anthonomus grandis) are putative receptors for the Cry1B protein of Bacillus thuringiensis.

    Science.gov (United States)

    Martins, Erica Soares; Monnerat, Rose Gomes; Queiroz, Paulo Roberto; Dumas, Vinicius Fiuza; Braz, Shélida Vasconcelos; de Souza Aguiar, Raimundo Wagner; Gomes, Ana Cristina Menezes Mendes; Sánchez, Jorge; Bravo, Alejandra; Ribeiro, Bergmann Morais

    2010-02-01

    Cry toxins from Bacillus thuringiensis (Bt) are used for insect control. They interact with specific receptors located on the host cell surface and are activated by host proteases following receptor binding resulting in midgut epithelial cells lysis. In this work we had cloned, sequenced and expressed a cry1Ba toxin gene from the B thuringiensis S601 strain which was previously shown to be toxic to Anthonomus grandis, a cotton pest. The Cry1Ba6 protein expressed in an acrystaliferous B. thuringiensis strain was toxic to A. grandis in bioassays. The binding of Cry1Ba6 toxin to proteins located in the midgut brush border membrane of A. grandis was analyzed and we found that Cry1Ba6 binds to two proteins (62 and 65kDa) that showed alkaline phosphatase (ALP) activity. This work is the first report that shows the localization of Cry toxin receptors in the midgut cells of A. grandis. 2009. Published by Elsevier Ltd.

  14. Effect of moisture availability on wood density and vessel characteristics of Eucalyptus grandis in the warm temperate region of South Africa

    CSIR Research Space (South Africa)

    Naidoo, Sasha

    2006-09-01

    Full Text Available intolerant of adverse conditions, and performs poorly when planted on shallow soils and/or on dry sites. A study was conducted to assess the effect of moisture availability on the wood density and vessel characteristics of E. grandis grown in the warm...

  15. Microarray-based method for the parallel analysis of genotypes and expression profiles of wood-forming tissues in Eucalyptus grandis

    CSIR Research Space (South Africa)

    Barros, E

    2009-05-01

    Full Text Available of Eucalyptus grandis planting stock that exhibit preferred wood qualities is thus a priority of the South African forestry industry. The researchers used microarray-based DNA-amplified fragment length polymorphism (AFLP) analysis in combination with expression...

  16. A technique to identify annual growth rings in Eucalyptus grandis using annual measurements of diameter at breast height and gamma ray densitometry

    CSIR Research Space (South Africa)

    Naidoo, Sasha

    2010-06-01

    Full Text Available A technique was developed to identify annual growth rings in E. grandis using a combination of annual measurements of diameter at breast height (DBH) from permanent sample plot (PSP) datasets and bark-pith density profiles. By assessing the pattern...

  17. ABO blood groups, Rhesus factor, and anaphylactic reactions due to Hymenoptera stings.

    Science.gov (United States)

    Pałgan, Krzysztof; Bartuzi, Zbigniew; Chrzaniecka, Elżbieta

    2017-09-21

    Numerous publications indicate that the prevalence of some infectious, neoplastic and immunological diseases are associated with ABO blood groups. The aim of this study was to verify whether ABO and Rh blood groups are associated with severe anaphylactic reactions after Hymenoptera stings. A study was undertaken of 71,441 Caucasian subjects living in the same geographic area. The study group included 353 patients with diagnosed systemic anaphylaxis to Hymenoptera venom. Control group included 71,088 healthy blood donors. Frequencies of ABO and Rhesus groups in the study and control groups were compared using univariate and multivariate analyses. No statistically significant interactions were observed between the ABO blood group and anaphylactic reactions to Hymenoptera.

  18. Apomictic parthenogenesis in a parasitoid wasp Meteorus pulchricornis, uncommon in the haplodiploid order Hymenoptera.

    Science.gov (United States)

    Tsutsui, Y; Maeto, K; Hamaguchi, K; Isaki, Y; Takami, Y; Naito, T; Miura, K

    2014-06-01

    Although apomixis is the most common form of parthenogenesis in diplodiploid arthropods, it is uncommon in the haplodiploid insect order Hymenoptera. We found a new type of spontaneous apomixis in the Hymenoptera, completely lacking meiosis and the expulsion of polar bodies in egg maturation division, on the thelytokous strain of a parasitoid wasp Meteorus pulchricornis (Wesmael) (Braconidae, Euphorinae) on pest lepidopteran larvae Spodoptera litura (Fabricius) (Noctuidae). The absence of the meiotic process was consistent with a non-segregation pattern in the offspring of heterozygous females, and no positive evidence was obtained for the induction of thelytoky by any bacterial symbionts. We discuss the conditions that enable the occurrence of such rare cases of apomictic thelytoky in the Hymenoptera, suggesting the significance of fixed heterosis caused by hybridization or polyploidization, symbiosis with bacterial agents, and occasional sex. Our finding will encourage further genetic studies on parasitoid wasps to use asexual lines more wisely for biological control.

  19. A new scenario of bioprospecting of Hymenoptera venoms through proteomic approach

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    LD Santos

    2011-01-01

    Full Text Available Venoms represent a huge and essentially unexplored reservoir of bioactive components that may cure diseases that do not respond to currently available therapies. This review select advances reported in the literature from 2000 to the present about the new scenario of Hymenoptera venom composition. On account of new technologies in the proteomic approach, which presents high resolution and sensitivity, the combination of developments in new instruments, fragmentation methods, strategic analysis, and mass spectrometry have become indispensable tools for interrogation of protein expression, molecule interaction, and post- translational modifications. Thus, the biochemical characterization of Hymenoptera venom has become a major subject of research in the area of allergy and immunology, in which proteomics has been an excellent alternative to assist the development of more specific extracts for diagnosis and treatment of hypersensitive patients to Hymenoptera venoms.

  20. Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae.

    Science.gov (United States)

    Artico, Sinara; Ribeiro-Alves, Marcelo; Oliveira-Neto, Osmundo Brilhante; de Macedo, Leonardo Lima Pepino; Silveira, Sylvia; Grossi-de-Sa, Maria Fátima; Martinelli, Adriana Pinheiro; Alves-Ferreira, Marcio

    2014-10-04

    Cotton is a major fibre crop grown worldwide that suffers extensive damage from chewing insects, including the cotton boll weevil larvae (Anthonomus grandis). Transcriptome analysis was performed to understand the molecular interactions between Gossypium hirsutum L. and cotton boll weevil larvae. The Illumina HiSeq 2000 platform was used to sequence the transcriptome of cotton flower buds infested with boll weevil larvae. The analysis generated a total of 327,489,418 sequence reads that were aligned to the G. hirsutum reference transcriptome. The total number of expressed genes was over 21,697 per sample with an average length of 1,063 bp. The DEGseq analysis identified 443 differentially expressed genes (DEG) in cotton flower buds infected with boll weevil larvae. Among them, 402 (90.7%) were up-regulated, 41 (9.3%) were down-regulated and 432 (97.5%) were identified as orthologues of A. thaliana genes using Blastx. Mapman analysis of DEG indicated that many genes were involved in the biotic stress response spanning a range of functions, from a gene encoding a receptor-like kinase to genes involved in triggering defensive responses such as MAPK, transcription factors (WRKY and ERF) and signalling by ethylene (ET) and jasmonic acid (JA) hormones. Furthermore, the spatial expression pattern of 32 of the genes responsive to boll weevil larvae feeding was determined by "in situ" qPCR analysis from RNA isolated from two flower structures, the stamen and the carpel, by laser microdissection (LMD). A large number of cotton transcripts were significantly altered upon infestation by larvae. Among the changes in gene expression, we highlighted the transcription of receptors/sensors that recognise chitin or insect oral secretions; the altered regulation of transcripts encoding enzymes related to kinase cascades, transcription factors, Ca2+ influxes, and reactive oxygen species; and the modulation of transcripts encoding enzymes from phytohormone signalling pathways. These

  1. PRODUTIVIDADE E CUSTOS DO PROCESSADOR TRABALHANDO EM POVOAMENTOS DE Eucalyptus grandis Hill ex Maiden

    Directory of Open Access Journals (Sweden)

    Carlos Cardoso Machado

    1999-12-01

    Full Text Available No presente trabalho foi realizado um estudo de tempos com o objetivo de ajustar equações para estimar o tempo das atividades, a produtividade, o custo operacional e da produção do processador utilizado em um sistema de colheita de madeira de povoamentos de Eucalyptus grandis Hill ex Maiden, em topografia plana, no Estado da Bahia. O ciclo operacional do processador consistiu no tempo gasto para processar uma árvore e foi dividido em etapas as quais foram cronometradas, usando a metodologia dos tempos individuais. A unidade amostral foi constituída do ciclo operacional da máquina. A análise estatística baseou-se em estudo de regressão, sendo utilizado o procedimento de modelagem de regressão "stepwise". Com as equações ajustadas, foi possível estimar a produtividade da máquina em função do diâmetro das árvores. Nas condições do estudo, considerando uma eficiência operacional de 70%, a produtividade do processador foi de 25,8 m³cc/h, o custo operacional 47,90 US$/h e o custo de produção de 1,86 US$/m³cc. Com base nos resultados obtidos, pode-se concluir que: o tempo de processamento das árvores varia diretamente com o aumento do diâmetro; o tempo de preparação, ao contrário do tempo de processamento, possui uma baixa correlação com o diâmetro das árvores; a produtividade do processador é diretamente proporcional ao aumento do diâmetro das árvores, quando expressa em volume, e inversamente proporcional, quando expressa em número de árvores; o custo por metro cúbico de madeira processada com processador, varia numa relação inversa ao aumento do diâmetro; do custo operacional do processador, o custo fixo é o de maior proporção, seguido do custo variável, administrativo e da mão-de-obra; e, o custo de produção do processador diminui em forma exponencial com o aumento do diâmetro das árvores.

  2. Espécies novas de Trigonisca Moure (Hymenoptera, Apidae, Apinae New species of Trigonisca Moure (Hymenoptera, Apidae, Apinae

    Directory of Open Access Journals (Sweden)

    Patrícia Maia Correia de Albuquerque

    2007-01-01

    Full Text Available Algumas espécies novas de Meliponini do gênero Trigonisca Moure, 1950 (Hymenoptera, Apidae, Apinae do Brasil e Panamá são descritas: T. roubiki sp. nov. (Panamá, T. variegatifrons sp. nov. (Brasil: RO, PA, MT, T. vitrifrons sp. nov. (Brasil: AM, PA, T. unidentata sp. nov. (Brasil: AM, T. meridionalis sp. nov. (Brasil: PA, MA, MT, MG, SP, T. bidentata sp. nov. (Brasil: RO, T. extrema sp. nov. (Brasil: AM e T. hirticornis sp. nov. (Brasil: RO, PA; e são apresentados novos registros geográficos de T. flavicans (Moure, 1950, T. intermedia Moure, 1989, T. dobzhanskyi (Moure, 1950, T. ceophloei (Schwarz, 1938, T. nataliae (Moure, 1950 e T. pediculana (Fabricius, 1804 e uma chave para identificação das espécies.Some new Meliponini species of the genus Trigonisca Moure, 1950 (Hymenoptera, Apidae, Apinae from Brazil and Panamá are described: T. roubiki sp. nov. (Panamá, T. variegatifrons sp. nov. (Brazil: RO, PA, MT, T. vitrifrons sp. nov. (Brazil: AM, PA, T. unidentata sp. nov. (Brazil: AM, T. meridionalis sp. nov. (Brazil: PA, MA, MT, MG, SP, T. bidentata sp. nov. (Brazil: RO, T. extrema sp. nov. (Brazil: AM and T. hirticornis sp. nov. (Brazil: RO, PA; additional geographic records from T. flavicans (Moure, 1950, T. intermedia Moure, 1989, T. dobzhanskyi (Moure, 1950, T. ceophloei (Schwarz, 1938, T. nataliae (Moure, 1950 and T. pediculana (Fabricius, 1804 and an identification key for species are presented.

  3. Systematics and evolution of the Meriones shawii/grandis complex (Rodentia, Gerbillinae) during the Late Quaternary in northwestern Africa: Exploring the role of environmental and anthropogenic changes

    Science.gov (United States)

    Stoetzel, Emmanuelle; Cornette, Raphaël; Lalis, Aude; Nicolas, Violaine; Cucchi, Thomas; Denys, Christiane

    2017-05-01

    Rodents of the Meriones shawii/grandis complex have been attested to in North Africa since the Middle Pleistocene and are abundant in archaeological sites. Today, they are widely spread and represent a major pest to local human populations. This complex, therefore, represents an accurate model for investigating the roles of climate change and human impact in shaping Quaternary faunal diversity and distribution. Many gray areas still exist regarding the systematics, ecology and geographical distribution of this complex, for both present and past populations. The purpose of this study is to compare modern genotyped and fossil Meriones specimens in order to 1) clarify the current systematics and distribution of the Meriones populations of the shawii/grandis complex, 2) document the taxonomic diversity in fossil Meriones from northwestern Africa, and 3) track their phenotypic and biogeographic evolution through time. To answer these questions we used geometric morphometrics on skulls (landmarks) and first upper molars (landmarks and sliding landmarks). We evidenced the existence of two morpho-groups within the M. shawii/grandis complex, with a clear geographic pattern (M. grandis in Morocco vs. M. shawii in Algeria and Tunisia). Currently only one morpho-group, attributed to M. grandis, seems to exist in Morocco, with a small overlap with M. shawii in the most eastern part of the country. However, according to fossil data, M. shawii was also present in Atlantic Morocco during the Late Pleistocene. We have also highlighted the impact of Holocene climate change and habitat anthropization on this arid adapted group. During the Middle Holocene, a major climatic event (last interglacial optimum) seems to have induced a demographic collapse in Moroccan populations and the disappearance of the shawii clade from Morocco (except in the most eastern areas). Both species then re-expanded, benefitting from the increasing aridity and the new ecological niche driven by agriculture

  4. Epidemiology, diagnosis, and treatment of Hymenoptera venom allergy in mastocytosis patients.

    Science.gov (United States)

    Niedoszytko, Marek; Bonadonna, Patrizia; Oude Elberink, Joanne N G; Golden, David B K

    2014-05-01

    Hymenoptera venom allergy is a typical IgE-mediated reaction caused by sensitization to 1 or more allergens of the venom, and accounts for 1.5% to 34% of all cases of anaphylaxis. Patients suffering from mastocytosis are more susceptible to the anaphylactic reactions to an insect sting. This article aims to answer the most important clinical questions raised by the diagnosis and treatment of insect venom allergy in mastocytosis patients. Total avoidance of Hymenoptera is not feasible, and there is no preventive pharmacologic treatment available, although venom immunotherapy reduces the risk of subsequent systemic reactions. Copyright © 2014 Elsevier Inc. All rights reserved.

  5. Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla Organogênese e transformação genética transiente do híbrido Eucalyptus grandis × Eucalyptus urophylla

    Directory of Open Access Journals (Sweden)

    Giovana Bomfim de Alcantara

    2011-04-01

    Full Text Available The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium every five days. This study also aimed at evaluating factors affecting the genetic transformation of leaf explants with the uidA gene, via co-cultivation with Agrobacterium tumefaciens, such as the pre-culture of the explants on a specific medium, the duration of their co-culture with the bacteria and the addition of acetosyringone to the culture media. The best conditions for the expression of the uidA gene were two days of pre-culture of the leaf tissues, three days of co-culture with the bacteria and the addition of acetosyringone in pre- and co-culture media.O híbrido Eucalyptus grandis x Eucalyptus urophylla apresenta alta produtividade e potencial para indústrias de papel, celulose e fibras. A organogênese de explantes foliares de dois clones de E. grandis × E. urophylla foi estudada para verificar fatores como tempo de repicagem das plântulas matrizes em meio de multiplicação; tipo de explantes, folhas inteiras e de meias-folhas (porções basais e apicais e dos dias que os explantes foliares permaneceram em meio de regeneração. Foram observadas diferenças na capacidade organogênica dos dois clones testados. A parte basal das folhas, coletadas de brotações repicadas a cada 17

  6. Behavior of Eucalyptus grandis and E. cloeziana seedlings grown in arsenic-contaminated soil Comportamento de mudas de E. grandis E. eucalyptus cloeziana cultivadas em solo contaminado por arsênio

    Directory of Open Access Journals (Sweden)

    Roseli Freire Melo

    2010-06-01

    Full Text Available Arsenic has been considered the most poisonous inorganic soil pollutant to living creatures. For this reason, the interest in phytoremediation species has been increasing in the last years. Particularly for the State of Minas Gerais, where areas of former mining activities are prone to the occurrence of acid drainage, the demand is great for suitable species to be used in the revegetation and "cleaning" of As-polluted areas. This study was carried out to evaluate the potential of seedlings of Eucalyptus grandis (Hill Maiden and E. cloeziana F. Muell, for phytoremediation of As-polluted soils. Soil samples were incubated for a period of 15 days with different As (Na2HAsO4 doses (0, 50, 100, 200, and 400 mg dm-3. After 30 days of exposure the basal leaves of E. cloeziana plants exhibited purple spots with interveinal chlorosis, followed by necrosis and death of the apical bud at the 400 mg dm-3 dose. Increasing As doses in the soil reduced root and shoot dry matter, plant height and diameter in both species, although the reduction was more pronounced in E. cloeziana plants. In both species, As concentrations were highest in the root system; the highest root concentration was found in E. cloeziana plants (305.7 mg kg-1 resulting from a dose of 400 mg dm-3. The highest As accumulation was observed in E. grandis plants, which was confirmed as a species with potential for As phytoextraction, tending to accumulate As in the root system and stem.O arsênio (As tem sido considerado o poluente inorgânico de solo mais tóxico para os seres vivos, razão pela qual o interesse por espécies indicadoras e fitorremediadoras tem aumentado nos últimos anos. Particularmente para o Estado de Minas Gerais, que apresenta áreas remanescentes de atividade mineradora sujeitas à ocorrência de drenagem ácida, existe grande demanda por espécies com potencial para serem utilizadas na revegetação e "limpeza" de substratos contaminados por esse metaloide. Este

  7. Estudo de algumas propriedades mecânicas da madeira de um híbrido clonal de Eucalyptus urophylla X Eucalyptus grandis Study of some mechanical properties of wood in a clonal hybrid of Eucalyptus urophylla X Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Fabrício Gomes Gonçalves

    2009-06-01

    Full Text Available A procura por madeiras oriundas de reflorestamentos destinadas à serraria é uma realidade já há muitos anos, principalmente aquelas das espécies do gênero Eucalyptus. Visando buscar novas informações importantes para esse mercado, este trabalho objetivou determinar algumas propriedades mecânicas da madeira de um híbrido clonal de Eucalyptus urophylla x Eucalyptus grandis de duas idades e provenientes de talhadia simples e de reforma. Os resultados indicaram que a madeira desse híbrido apresenta boas características tecnológicas, destacando-se a segunda tora (a partir de 3 m com as melhores propriedades de flexão estática (Módulo de Elasticidade - MOE e Módulo de Ruptura - MOR e Compressão Axial das fibras. As árvores de maior idade (166 meses e que sofreram dois desbastes apresentaram as melhores propriedades de flexão estática e compressão axial.The search for wood originated from reforestations destined to sawmill has been observed for many years, mainly for the Eucalyptus species. Searching for information to assist this market, the aim of this study was to determine some mechanical properties of the wood from a Eucalyptus urophylla x Eucalyptus grandis clonal hybrid, at two different ages and coming from coppice and reform. The results show that the wood of this clonal hybrid presents good technological characteristics and is the second best log (starting from 3,0 m, as to static bending properties (Module of Elasticity - MOE and Module of Rupture - MOR. The oldest trees (166 months - E2 which suffered two thinning presented the best results for the property of static bending and axial crushing (CA.

  8. EFFECT OF THINNING AND FERTILIZATION ON Eucalyptus grandis Hill ex-Maiden PITH DISPLACEMENT AND LOG END SPLITTlNG

    Directory of Open Access Journals (Sweden)

    Israel Luiz de Lima

    2007-06-01

    Full Text Available The aim of this study was to analyse the influence of thinning and fertilization on some log properties which influence sawn wood yield in a 21 year-old grown Eucalyptus grandis stand. Three selective thinning intensities (37, 50 and 75%, presence or absence of fertilizers on the beginning of the thinning, two diameter classes and two logs taken from two different vertical positions were taken as the study factors. The influence of the factors and their combination on tree growth stresses were indirectly evaluate through log end splitting and pith displacement in relation to its normal central position. Thinning, fertilization, diameter class and log position on the tree height did not have significant effect on pith displacement. The logs from fertilized plots presented higher log end splitting index than others.

  9. Genetic diversity of Brazilian natural populations of Anthonomus grandis Boheman (Coleoptera: Curculionidae), the major cotton pest in the New World.

    Science.gov (United States)

    Martins, W F S; Ayres, C F J; Lucena, W A

    2007-01-27

    Twenty-five RAPD loci and 6 isozyme loci were studied to characterize the genetic variability of natural populations of Anthonomus grandis from two agroecosystems of Brazil. The random-amplified polymorphic DNA data disclosed a polymorphism that varied from 52 to 84% and a heterozygosity of 0.189 to 0.347. The index of genetic differentiation (GST) among the six populations was 0.258. The analysis of isozymes showed a polymorphism and a heterozygosity ranging from 25 to 100% and 0.174 to 0.277, respectively. The genetic differentiation (FST) among the populations obtained by isozyme data was 0.544. It was possible to observe rare alleles in the populations from the Northeast region. The markers examined allowed us to distinguish populations from large-scale, intensive farming region (cotton belts) versus populations from areas of small-scale farming

  10. Biomass of Eucalyptus grandis coffs harbour, by growth-ring identification using the gamma-ray attenuation technique

    International Nuclear Information System (INIS)

    Rezende, M.A.

    1984-01-01

    Samples of 13-year old Eucalyptus grandis were analysed using the gamma-ray attenuation technique. The density was obtained through the profile, and it was then possible to determine the mean density for each annual ring of the samples. The period from formation of two consecutive late-wood rings, i.e., two high density peaks, was considered as one year of tree life. An attempt was made to determine the annual increments at breast-heigh diameter, per height unit, based on volume, mass and energy, trying to estimate the cutting age for each of these elements and the relationship between them. Emphasis was also put on the importance with which density variations, and core to cork calorific value, should be considered when determining the cutting age. (M.A.C.) [pt

  11. CANONICAL CORRELATION OF PHYSICAL AND CHEMICAL CHARACTERISTICS OF THE WOOD OF Eucalyptus grandis AND Eucalyptus saligna CLONES

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    Paulo Fernando Trugilho

    2003-01-01

    Full Text Available The analysis of canonical correlation measures the existence and the intensity of the association between two groups of variables. The research objectified to evaluate thecanonical correlation between chemical and physical characteristics and fiber dimensional ofwood of Eucalyptus grandis and Eucalyptus saligna clones, verifying the interdependenceamong the groups of studied variables. The analysis indicated that the canonical correlationswere high and that in two cases the first and second pair were significant at the level of 1% ofprobability. The analysis of canonical correlation showed that the groups are notindependent. The intergroup associations indicated that the wood of high insoluble lignin contentand low ash content is associated with the high radial and tangential contraction and highbasic density wood.

  12. SITE INDEX CURVES AND HYPSOMETRIC RELATIONSHIP FOR Eucalyptus grandis PLANTATIONS FOR THE CAMPOS GERAIS REGION, PARANA STATE

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    Fabiane Aparecida de Souza Retslaff

    2015-06-01

    Full Text Available The study aimed to fit mathematical models for the construction of Site Index curves and to estimate heights at different ages for Eucalyptus grandis in the Campos Gerais region, Parana State. The data used to fit the models came from permanent, temporary plots and pre-harvesting inventory, covering ages from 2.5 to 26.5 years. Several models were tested to represent the sites and the hypsometric relationship. The Site Index curves were constructed by the guide-curve method. For the Site Index, the Chapman-Richards model showed the best fit and precision statistics, generating 5 Site Index curves (range of 5 m with the Chapman-Richards model. The four hypsometric models tested showed satisfactory performance and similar statistics and the inclusion of the variables dominant height or site index did not substantially improve the goodness of fit statistics, but the residues were more homogeneous and closer to zero.

  13. A raptor study for the proposed Grandy Brook Station to Hope Brook Mine transmission line environmental impact statement

    Energy Technology Data Exchange (ETDEWEB)

    1986-01-01

    The proposed Grandy Brook Station to Hope Brook mine transmission line study area occupies a remote area of south-central Newfoundland. Raptors have been identified as a valued ecosystem component which may be adversely affected. Since little information on population numbers and activities in the study area is available, a survey was conducted to determine raptor use of the proposed route to minimize adverse impacts of the development. Field data was the primary information source, although government officials were consulted for additional information and data files were accessed. Raptors were recorded by species and age class, and apparent activity. One field trip, composed of a ground survey and a helicopter survey, was conducted on July 12, 1986. Suggestions are made for mitigation measures related to such negative aspects as electrocution during construction and maintenance activities, harassment and persecution, collisions with the lines, and electric fields and corona.

  14. Effect of Maruca vitrata (Lepidoptera: Crambidae) host plants on life-history parameters of the parasitoid Apanteles taragamae (Hymenoptera: Braconidae)

    NARCIS (Netherlands)

    Dannon, A.E.; Tamo, M.; Agboton, C.; Huis, van A.; Dicke, M.

    2012-01-01

    The effect of four host plant species of the herbivore Maruca vitrata Fabricius (Lepidoptera: Crambidae) on development time, longevity, fecundity and sex ratio of the parasitoid Apanteles taragamae Viereck (Hymenoptera: Braconidae) was investigated under laboratory conditions. The larvae were

  15. Hyperparasitoid wasps (Hymenoptera: Trigonalidae) reared from dry forest and rain forest caterpillars of Area de Conservacion, Guanacaste, Costa Rica

    Science.gov (United States)

    Five species of Trigonalidae, hyperparasites of Ichneumonidae (Hymenoptera) and Tachinidae (Diptera) that parasitize caterpillars (Lepidoptera), have been reared during the ongoing caterpillar inventory of Area de Conservación Guanacaste (ACG), Guanacaste Province, northwestern Costa Rica: Lycogaste...

  16. Role of emerald ash borer (Coleoptera: Buprestidae) larval vibrations in host-quality assessments by Tetrastichus planipennisi (Hymenoptera: Eulophidae)

    Science.gov (United States)

    Michael D. Ulyshen; Richard W. Mankin; Yigen Chen; Jian J. Duan; Therese M. Poland; Leah S. Bauer

    2011-01-01

    The biological control agent Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) is a gregarious larval endoparasitoid of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive cambium-feeding species responsible for recent, widespread mortality of ash (Fraxinus spp.) in...

  17. Reproductive and developmental biology of the emerald ash borer parasitoid Spathius galinae (Hymenoptera: Braconidae) as affected by temperature

    Science.gov (United States)

    Emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive pest of serious concern in North America. To complement ongoing biological control efforts, Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a recently-described specialist parasitoid of ...

  18. Linking changes in subcellular cadmium distribution to growth and mortality rates in transplanted freshwater bivalves (Pyganodon grandis)

    International Nuclear Information System (INIS)

    Perceval, Olivier; Couillard, Yves; Pinel-Alloul, Bernadette; Campbell, Peter G.C.

    2006-01-01

    Relationships between Cd accumulation and subcellular distribution, and growth and mortality rates were examined in the freshwater bivalve Pyganodon grandis in a transplant experiment. Organisms were transferred from a clean lacustrine site to four lakes situated along a Cd concentration gradient in the mining region of Rouyn-Noranda. The bivalves were maintained in open enclosures placed in the bottom sediments of the littoral zone of all five lakes for 400 days. At the end of the experiment, metallothionein (MT) was measured in the bivalve gills with a Hg-saturation assay and Cd partitioning among the various cytosolic protein pools was determined by size-exclusion chromatography. Marked differences were observed among the five sites: the range in calculated free-cadmium ion concentrations in water overlying the sediments was 35-fold whereas Cd concentrations in the gill cytosol of the transplanted bivalves varied three-fold. In the transplanted bivalves, the distribution of gill Cd among the various cytosolic complexes also varied significantly among sites. For bivalves transplanted to the three most contaminated sites, Cd concentrations in the high molecular weight pool (HMW > 25 kDa) were significantly higher than the baseline levels determined from bivalves caged at the reference site; a similar trend was seen for Cd concentrations in the metallothionein pool (Cd-MT). For bivalves transferred to two of the high contamination sites, proportionately less of the gill cytosolic Cd was sequestered (i.e. detoxified) by MT-like proteins. Reductions in survival were also observed at these two sites, and these elevated mortalities, in turn, were consistent with the absence of indigenous bivalve populations at these sites. This result is compatible with our recent work on P. grandis populations living in lakes of the Rouyn-Noranda area, in which we demonstrated that excessive accumulation of Cd in the HMW pool of the gill cytosol of the individual mollusks could be

  19. EFEITO DA TERMORRETIFICAÇÃO NAS PROPRIEDADES MECÂNICAS DAS MADEIRAS DE Pinus taeda E Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Karina Soares Modes

    2017-01-01

    Full Text Available This study investigated the effect of thermal treatment, employed under two treatment conditions on mechanical properties of wood of Pinus taeda L. and Eucalyptus grandis W. Hill ex Maiden. From each species, three 25-year tress were sampled, and from each one, it was taken the first 2-meter long log, which was obtained from two boards of size 7,0 x 20,0 cm (thickness x width respectively diametrically opposite from where 30 specimens were saw for each treatment. At the first one, called the combined one, the woods were subjected to heat treatment by autoclaving at 130 / ± 3°C and pressure of 2 kgf / cm ² for 3 hours and, after a conditioning period, subjected to heat in an electric oven at 160 / ± 1°C for the same period. The second treatment consisted only of heat treatment in oven. It was also evaluated the pieces of wood without treatment (control. The mechanical properties were evaluated by means of tests for determining the modulus of elasticity and rupture in bending, maximum resistance to compression parallel to the grain and Janka hardness according to ASTM D 143 (1995, and the impact resistance according to ABNT NBR 7190 (1997. For Pinus taeda wood, it was observed that treatment in an oven gave the worst outcomes, both due to the reduction in the values of supported load of a greater number of mechanical properties evaluated, but also as compared to the lowest increments in resistance when it was observed an increase to the same ones with heat treatment. In Eucalyptus grandis, the combined treatment decreased the greatest number of mechanical properties of wood.

  20. Potensi Kayu Perkakas dan Kayu Bakar Jenis Jati (Tectona grandis di Hutan Rakyat Desa Natah, Gunung Kidul

    Directory of Open Access Journals (Sweden)

    Ris Hadi Purwanto

    2009-07-01

    Full Text Available Potentials  of Merchantable Timber and Firewod of Teak (Tectona grandis in Natah Village Community Forest , Gunungkidul The potential of merchantable timber and firewod of teak (Tectona grandis in Desa Natah community forest was estimated by developing allometric equations method. To establish the allometric equation 350 sample trees were measured to determine the relationships between tree height (H and diameter breast height (D. Thirty trees of various sizes were cut to measure the merchantable timber and firewood volume. The raw merchantable timber volume of teak in the community forest was defined as the ligneous material contained in the bole and branches which both with a diameter of at least 10cm. The result showed that D (taken at about 1.3m above the ground was a good predictor of H with r2 over 0.9672. When D was combined with H, r2 was improved somewhat for the merchantable timber volume, suggesting the growth patterns of tree dimensions were colesy interdependent. A standing stock of the merchantable timber and firewood volume of teak in the community forest was then estimated based on the allometric relations. Proportions of the merchantable timber and firewood volume were 66.91% and 33.09% of total wood volume per tree, respectively. The potential of merchantable timber and firewood volume in these community forest were 13.501 m3/ha and 8.686 m3/ha, respectively, with a basal area of 1.887 m2/ha. Based on the basal area, Desa Natah community forests of teak could be classified into extremely sparse of stands category.

  1. Phytochemical profile, aldose reductase inhibitory, and antioxidant activities of Indian traditional medicinal Coccinia grandis (L.) fruit extract.

    Science.gov (United States)

    Kondhare, Dasharath; Lade, Harshad

    2017-12-01

    Coccinia grandis (L.) fruits (CGFs) are commonly used for culinary purposes and has several therapeutic applications in the Southeast Asia. The aim of this work was to evaluate phytochemical profile, aldose reductase inhibitory (ARI), and antioxidant activities of CGF extract. The CGFs were extracted with different solvents including petroleum ether, dichloromethane, acetone, methanol, and water. The highest yield of total extractable compounds (34.82%) and phenolic content (11.7 ± 0.43 mg of GAE/g dried extract) was found in methanol extract, whereas water extract showed the maximum content of total flavonoids (82.8 ± 7.8 mg QE/g dried extract). Gas chromatography-mass spectroscopy (GC-MS) analysis of methanol and water extract revealed the presence of flavonoids, phenolic compounds, alkaloids, and glycosides in the CGFs. Results of the in vitro ARI activity against partially purified bovine lens aldose reductase showed that methanol extract of CGFs exhibited 96.6% ARI activity at IC 50 value 6.12 µg/mL followed by water extract 89.1% with the IC 50 value 6.50 µg/mL. In addition, methanol and water extracts of CGF showed strong antioxidant activities including ABTS *+ scavenging, DPPH* scavenging, and hydroxyl radical scavenging. Our results suggest that high percentage of both flavonoids and phenolic contents in the CGFs are correlated with the ARI and antioxidant activities. The fruits of C. grandis are thus potential bifunctional agents with ARI and antioxidant activities that can be used for the prevention and management of DM and associated diseases.

  2. MODELOS PREDICTIVOS DE BIOMASA AÉREA DE Eucalyptus grandis PARA EL NORESTE DE ARGENTINA

    Directory of Open Access Journals (Sweden)

    Rosa Angela Winck

    2015-01-01

    Full Text Available Diferentes intereses industriales, energéticos y ambientales han motivado el análisis de las distintas posibilidades de aprovechamiento de la biomasa forestal, este tema está adquiriendo un creciente protagonismo, por lo que la cuantificación de la biomasa aérea de estos sistemas forestales constituye un primer paso fundamental e ineludible. El objetivo de este trabajo fue desarrollar modelos que permitan estimar la biomasa de los diferentes compartimentos aéreos de Eucalyptus grandis . Para ello se emplearon datos de 41 árboles provenientes de rodales de diferentes edades, entre 4 y 32 años, ubicados en la región NE de Argentina. Se tomaron 23 individuos ubicados en la Zona Norte y Alta de Misiones (zona 1 y 18 en el Sur de Misiones y NE de Corrientes (zona 2. Se determinó la biomasa de hojas, ramas menores a 5 cm, ramas mayores a 5 cm, total de ramas, fuste y biomasa total del árbol. Para el ajuste de modelos se tomaron diversas variables independientes, el diámetro a la altura de pecho (dap, la altura total del árbol (h, el producto (dap 2 *h y la variable “ dummy” (zona. Para el caso de la biomasa de ramas y de hojas, debido a que no se lograron resultados satisfactorios con el empleo de estas variables, se examinaron modelos que adicionaban índice de sitio, factor de espaciamiento, diámetro en la base de la copa verde (dbcv, altura hasta la base de la copa verde (hbcv, longitud de copa verde (lcv, el área transversal de albura en la base de copa verde (g Alb bcv y diferentes combinaciones de estas variables. Los modelos fueron seleccionados en base al coeficiente de determinación y el error cuadrático medio. Para la biomasa de ramas menores a 5 cm, ramas mayores a 5 cm, total de ramas, fuste y total, los mejores modelos fueron los que incorporaron como variables regresoras, el diámetro a la altura de pecho, la altura total del árbol y la variable “ dummy” (zona. Para la biomasa de hojas, las variables, edad,

  3. MANEJO DE RESIDUOS DE Eucalyptus grandis Hill ex Maiden EN SITIOS CONTRASTANTES

    Directory of Open Access Journals (Sweden)

    Ana Maria Lupi

    2017-01-01

    Full Text Available En la región NE de Argentina existe poca información sobre el impacto que tienen las diferentes alternativas de manejo de residuos de la cosecha forestal sobre las propiedades edáficas. En este estudio se analizó el efecto a corto plazo de sistemas de manejo de residuos de la cosecha de Eucalyptus grandis Hill ex Maiden, sobre la concentración del carbono orgánico total del suelo (COT y la fracción particulada (COP, su estado estructural y la conductividad hidráulica saturada. El estudio se desarrolló en dos sitios de la Provincia de Entre Ríos, Argentina, en un suelo Aquic Hapluderts (S1 y en un Fluventic Hapludolls (S2. Se aplicaron los siguientes tratamientos: 1. Extracción total de residuos (ER, 2. Conservación de residuos (CR, 3. Quema de residuos-testigo (QR, 4. Doble adición de residuos (DAR. El muestreo de suelos en las profundidades 0-5 cm y 5-10 cm se realizó a los 18 (S1 y a los 22 (S2 meses de aplicar los tratamientos. En el corto plazo, el efecto de los tratamientos dependió del tipo de suelo y de la variable analizada. Los contrastes más importantes se debieron al efecto de las características intrínsecas del suelo sobre el tamaño de los agregados y la conductividad hidráulica saturada. La conservación de residuos no aumentó la concentración de COT ni modificó la calidad de la materia orgánica medida a partir de COP. El suelo Aquic Hapluderts presentó agregados más grandes, más estables y una mayor K sat en comparación con el Fluventic Hapludolls. En el Aquic Hapluderts se observó una mayor conductividad hidráulica saturada en los tratamientos con conservación de los residuos (DAR y CR, debido a que la cobertura preservaría el sistema poroso en la interfase suelo-atmosfera. El Fluventic Hapludolls fue menos estable y los tratamientos no modificaron significativamente el tamaño de los agregados ni la conductividad hidráulica saturada . Considerando que las características del sitio tienen efectos

  4. First record of Aleiodes laphygmae (Hymenoptera: Braconidae) for Argentina and its association with larvae of Spodoptera eridania (Lepidoptera: Noctuidae)

    OpenAIRE

    Valverde, Liliana; Berta, D. Carolina; Gomez, Marcelo Geronimo

    2012-01-01

    Se reporta por primera vez para Argentina Aleiodes laphygmae (Viereck) (Hymenoptera: Braconidae) y como parasitoide de larvas de Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae), en plantaciones de soja en la provincia de Tucumán (Argentina). Se provee información biológica como hábitos, hospedadores y distribución.Aleiodes laphygmae (Viereck) (Hymenoptera: Braconidae), is reported for the first time for Argentina. It is also reported parasitizing larvae of Spodoptera eridania (Stoll) (Le...

  5. Bumble bee (Hymenoptera: Apidae) community structure on two sagebrush steppe sites in southern Idaho

    Science.gov (United States)

    Stephen P. Cook; Sara M. Birch; Frank W. Merickel; Carrie Caselton Lowe; Deborah Page-Dumroese

    2011-01-01

    Although sagebrush, Artemisia spp., does not require an insect pollinator, there are several native species of bumble bees, Bombus spp. (Hymenoptera: Apidae), that are present in sagebrush steppe ecosystems where they act as pollinators for various forbs and shrubs. These native pollinators contribute to plant productivity and reproduction. We captured 12 species of...

  6. The species of the genus Hypodynerus de Saussure (Hymenoptera, Vespidae, Eumeninae occurring in Brazil

    Directory of Open Access Journals (Sweden)

    Bolívar Garcete-Barrett

    2013-04-01

    Full Text Available An identification table and descriptions are given to recognize the two species of Hypodynerus (Hymenoptera: Vespidae: Eumeninae recorded from Brazil: H. arechavaletae (Brèthes and H. duckei (Bertoni comb. n. The lectotype is designated and the male is described for Hypodynerus duckei, its presence being recorded from Brazil for the first time.

  7. The species of the genus Hypodynerus de Saussure (Hymenoptera, Vespidae, Eumeninae) occurring in Brazil.

    Science.gov (United States)

    Garcete-Barrett, Bolívar R; Hermes, Marcel Gustavo

    2013-01-01

    An identification table and descriptions are given to recognize the two species of Hypodynerus (Hymenoptera: Vespidae: Eumeninae) recorded from Brazil: Hypodynerus arechavaletae (Brèthes) and Hypodynerus duckei (Bertoni) comb. n. The lectotype is designated and the male is described for Hypodynerus duckei, its presence being recorded from Brazil for the first time.

  8. [Prevalence of hymenoptera sting allergy in veterinary medicine students from Monterey, Nuevo Leon, Mexico].

    Science.gov (United States)

    Arias Cruz, Alfredo; Monsiváis Toscano, Gina; Gallardo Martínez, Gabriela; González Díaz, Sandra Nora; Galindo Rodríguez, Gabriela

    2007-01-01

    The reported prevalence of allergic systemic reactions to hymenoptera venom occur in up to 3.3% and large local reactions occur in 17% in the general population. To investigate the prevalence of hymenoptera sting allergy in a group of veterinary medicine students from Monterrey, Nuevo Leon, Mexico. A transverse and observational study was done with 64 students of veterinary medicine. We conducted a questionnaire about the students' history of insect allergy and atopy. Skin test with allergenic extracts of bee and ant were practiced to all subjects. We performed aeroallergen skin prick test to the subjets with suspected atopy. Students age ranged from 17 to 25 years (mean 20.2) and 37 were males. Twenty students (31.3%) had clinical history of atopy and positive skin tests to aeroallergens. On the other hand, 5 students (7.8%), including 2 atopic, had suffered large local reactions, but none of them had suffered systemic reactions. Bee and ant skin tests were positive in 15.6% and 31.3% of the students respectively. There was no difference in the prevalence of hymenoptera allergy between atopic and non atopic subjects (p < 0.05). Further, the frequency of atopy in subjects with positive skin tests for bee and ant was 50%. The prevalence of large local reactions and hymenoptera sensitization found in this group was similar to that found in other epidemiologic studies.

  9. First report of Eurytoma plotnikovi Nik. (Hymenoptera, Eurytomidae, a seed parasite of pistachio, in Sicily (Italy

    Directory of Open Access Journals (Sweden)

    Santi Longo

    2011-09-01

    Full Text Available The pistachio seed wasp, Eurytoma plotnikovi Nik.(Hymenoptera, E urytomidae, is a new pest recently arrived in pistachio orchards in central-western Sicily (Italy. Information on the damaging effects of this seed wasp in the affected areas is provided.

  10. Invasive ant Tapinoma melanocephalum (Hymenoptera: Formicidae): A rare quest or increasingly common indoor pest in Europe?

    Czech Academy of Sciences Publication Activity Database

    Klimeš, Petr; Okrouhlík, Jan

    2015-01-01

    Roč. 112, č. 4 (2015), s. 705-712 ISSN 1210-5759 R&D Projects: GA ČR GB14-36098G Institutional support: RVO:60077344 Keywords : Hymenoptera * Formicidae * Tapinoma Subject RIV: EH - Ecology, Behaviour Impact factor: 0.975, year: 2014 http://www.eje.cz/pdfs/eje/2015/04/16.pdf

  11. The species of the Neotropical genus Fractipons Townes, 1970 (Hymenoptera, Ichneumonidae, Cryptinae).

    Science.gov (United States)

    Bordera, Santiago; González-Moreno, Alejandra

    2011-01-19

    In this paper, two new species of the Neotropical genus Fractipons Townes, 1970 (Hymenoptera, Ichneumonidae) are described. A new diagnosis for the genus, a re-description of Fractipons cincticornis Townes, 1970 and a key to known species are provided. New distribution records for the genus now include Argentina, Costa Rica, Panama and Peru.

  12. DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini)

    OpenAIRE

    Rocha, MP; Pompolo, SD; Dergam, JA; Fernandes, A; Campos, LAD

    2002-01-01

    We analyzed patterns of heterochromatic bands in the Neotropical stingless bee genus Melipona (Hymenoptera, Meliponini). Group I species (Melipona bicolor bicolor, Melipona quadrifasciata, Melipona asilvae, Melipona marginata, Melipona subnitida) were characterized by low heterochromatic content. Group 11 species (Melipona capixaba, Melipona compressipes, Melipona crinita, Melipona seminigra fuscopilosa e Melipona scutellaris) had high heterochromatic content. All species had 2n = 18 and n = ...

  13. The Hymenopterous Poison Apparatus. X. Morphological and Behavioral Changes in Atta texana (Hymenoptera: Formicidae)

    Science.gov (United States)

    Henry R. Hermann; John C. Moser; Allen N. Hunt

    1970-01-01

    Atta texana (Buckley) and other members of this genus no longer utilize the 8th and 9th gonapophyses as part of their defensive system. Although the sclerites that comprise the stinging apparatus in most aculeate Hymenoptera are present in the species, they seem to function only in the deposition of trail pheromones. A mechanical and chemical defense...

  14. Epidemiology, Diagnosis, and Treatment of Hymenoptera Venom Allergy in Mastocytosis Patients

    NARCIS (Netherlands)

    Niedoszytko, Marek; Bonadonna, Patrizia; Oude Elberink, Joanne N. G.; Golden, David B. K.

    Hymenoptera venom allergy is a typical IgE-mediated reaction caused by sensitization to 1 or more allergens of the venom, and accounts for 1.5% to 34% of all cases of anaphylaxis. Patients suffering from mastocytosis are more susceptible to the anaphylactic reactions to an insect sting. This article

  15. Application of recombinant antigen 5 allergens from seven allergy-relevant Hymenoptera species in diagnostics

    DEFF Research Database (Denmark)

    Schiener, Maximilian; Eberlein, Bernadette; Moreno Aguilar, Carmen

    2017-01-01

    BACKGROUND: Hymenoptera stings can cause severe anaphylaxis in untreated venom-allergic patients. A correct diagnosis regarding the relevant species for immunotherapy is often hampered by clinically irrelevant cross-reactivity. In vespid venom allergy, cross-reactivity between venoms of different...

  16. The spatial distribution of Hymenoptera parasitoids in a forest reserve in Central Amazonia, Manaus, AM, Brazil

    Directory of Open Access Journals (Sweden)

    RB. Querino

    Full Text Available Parasitoids are of great importance to forest ecosystems due to their ecological role in the regulation of the population of other insects. The species richness and abundance of parasitoids in the forest canopy and understory, both on the borders and in the interior of a tropical forest reserve in Central Amazonia were investigated. For a 12-month period, specimen collections were made every 15 days from suspended traps placed in the forest canopy and in the understory strata, both on the border and in the interior of forest areas. A total of 12,835 Hymenoptera parasitoids from 23 families were acquired. Braconidae, Diapriidae, Mymaridae, Eulophidae, and Scelionidae were the most represented in the area and strata samples. The results indicate that there were no significant differences in the species richness or abundance of Hymenoptera between the forest borders and the inner forest. The data does show that the presence of Hymenoptera is significantly greater in the understory in both the border and interior areas than in the canopy (vertical stratification. Aphelinidae and Ceraphronidae were significantly associated with the inner forest, while the other seven families with the border of the reserve. The abundance of Hymenoptera parasitoids presented seasonal variations during the year related to the rainy and dry seasons.

  17. Revision of the subfamily Euphorinae (excluding the tribe Meteorini Cresson) (Hymenoptera: Braconidae) from China

    NARCIS (Netherlands)

    Chen, X.; Achterberg, van C.

    1997-01-01

    The subfamily Euphorinae (excluding the tribe Meteorini Cresson) (Hymenoptera: Braconidae) from China is revised. In total 150 species, belonging to 24 genera, are treated and keyed. One genus (Heia gen. nov.; type species: Heia robustipes spec. nov.) and 69 species are described as new to science.

  18. Stiff upper lip: Labrum deformity and functionality in bees (Hymenoptera: Apoidea)

    Science.gov (United States)

    In hyper-diverse groups such as Hymenoptera, a variety of structures with different, complementary functions are used for feeding. Although the function of the parts such as the mandibles is obvious, the use of others, like the labrum, is more difficult to discern. Here, we discuss the labrum’s func...

  19. De kortsnuitbloedbij Sphecodes majalis nieuw voor de Nederlandse fauna (Hymenoptera: Apidae)

    NARCIS (Netherlands)

    Raemakers, I.

    2004-01-01

    Sphecodes majalis, a new bee species for the Netherlands (Hymenoptera: Apidae) A population of Sphecodes majalis was found on a limestone grassland near Maastricht (Limburg). On several occasions more than 10 female and several male specimen were observed. Sphecodes majalis is a parasite of

  20. De roodrandzandbij Andrena rosae in de Zuid-Hollandse Biesbosch (Hymenoptera: Apoidea: Andrenidae).

    NARCIS (Netherlands)

    Meer, van der F.; Reemer, M.; Peeters, T.M.J.

    2006-01-01

    Andrena rosae in the Zuid-Hollandse Biesbosch (Hymenoptera: Apoidea: Andrenidae) Andrena rosae is a fairly large, black and red mining bee. It has declined dramatically in the 20th century in northwestern Europe. In the Netherlands the species only survived in and around the Biesbosch, at the

  1. Revision of charipine aphid hyperparasitoids (Hymenoptera, Cynipoidea: Figitidae) from central Europe

    Czech Academy of Sciences Publication Activity Database

    Ferrer-Suay, M.; Starý, Petr; Selfa, J.; Pujade-Villar, J.

    2017-01-01

    Roč. 28, č. 3 (2017), s. 113-147 ISSN 0785-8760 Institutional support: RVO:60077344 Keywords : Hymenoptera * central Europe * aphid Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 0.300, year: 2016 http://www.entomologicafennica.org/Volume28/EF_28_3/1Ferrer-Suay.pdf

  2. Sting microsculpture in the digger wasp Bembix rostrata (Hymenoptera, Crabronidae

    Directory of Open Access Journals (Sweden)

    Natalia Matushkina

    2011-03-01

    Full Text Available The sting microsculpture of the digger wasp Bembix rostrata (Fabricius, 1781 (Hymenoptera, Crabronidae is studied with the scanning electron microscope (SEM for the first time. As in many other hymenopterans, the second valvifer of B. rostrata possesses two fields of styloconic sensilla (hair plates of proprioceptive function. The presence of two paired fields of campaniform sensilla on the second valvula and second valvifer is first shown in an apoid wasp. The first and the second valvulae bear scattered sensilla-like structures on the external surface, more numerous apically. The first valvula has two subapical barbs externally and a pair of valvilli on its inner surface, whereas the outer surface of the second valvula is smooth. The third valvula is sclerotized externally, consisting of proximal and distal parts, and bearing four sensilla morphotypes of mechanoreceptive and probably chemoreceptive functions. The inner surface of the valvulae and the membranous cuticle that is touching the sting have microstructures of different shapes directed distally. Functional aspects of characters studied are discussed.

  3. Function and evolution of microRNAs in eusocial Hymenoptera

    Directory of Open Access Journals (Sweden)

    Eirik eSovik

    2015-05-01

    Full Text Available The emergence of eusociality (true sociality in several insect lineages represents one of the most successful evolutionary adaptations in the animal kingdom in terms of species richness and global biomass. In contrast to solitary insects, eusocial insects evolved a set of unique behavioral and physiological traits such as reproductive division of labor and cooperative brood care, which likely played a major role in their ecological success. The molecular mechanisms that support the social regulation of behavior in eusocial insects, and their evolution, are mostly unknown. The recent whole-genome sequencing of several eusocial insect species set the stage for deciphering the molecular and genetic bases of eusociality, and the possible evolutionary modifications that led to it. Studies of mRNA expression patterns in the brains of diverse eusocial insect species have indicated that specific social behavioral states of individual workers and queens are often associated with particular tissue-specific transcriptional profiles. Here we discuss recent findings that highlight the role of non-coding microRNAs (miRNAs in modulating traits associated with reproductive and behavioral divisions of labor in eusocial insects. We provide bioinformatic and phylogenetic data, which suggest that some Hymenoptera-specific miRNA may have contributed to the evolution of traits important for the evolution of eusociality in this group.

  4. Foraging strategies of the ant Ectatomma vizottoi (Hymenoptera, Formicidae

    Directory of Open Access Journals (Sweden)

    Luan D. Lima

    2013-12-01

    Full Text Available Foraging strategies of the ant Ectatomma vizottoi (Hymenoptera, Formicidae. Foraging activity may be limited by temperature, humidity, radiation, wind, and other abiotic factors, all of which can affect energy costs during foraging. Ectatomma vizottoi's biology has only recently been studied, and no detailed information is available on its foraging patterns or diet in the field. For this reason, and because foraging activity is an important part of the ecological success of social insects, the present study aimed to investigate E. vizottoi's foraging strategies and dietary habits. First, we determined how abiotic factors constrained E. vizottoi's foraging patterns in the field by monitoring the foraging activity of 16 colonies on eight different days across two seasons. Second, we characterized E. vizottoi's diet by monitoring another set of 26 colonies during peak foraging activity. Our results show that E. vizottoi has foraging strategies that are similar to those of congeneric species. In spite of having a low efficiency index, colonies adopted strategies that allowed them to successfully obtain food resources while avoiding adverse conditions. These strategies included preying on other ant species, a foraging tactic that could arise if a wide variety of food items are not available in the environment or if E. vizottoi simply prefers, regardless of resource availability, to prey on other invertebrates and especially on other ant species.

  5. Preservation of Domesticated Honey Bee (Hymenoptera: Apidae) Drone Semen.

    Science.gov (United States)

    Paillard, M; Rousseau, A; Giovenazzo, P; Bailey, J L

    2017-08-01

    Preservation of honey bee (Apis mellifera L., Hymenoptera: Apidae) sperm, coupled with instrumental insemination, is an effective strategy to protect the species and their genetic diversity. Our overall objective is to develop a method of drone semen preservation; therefore, two experiments were conducted. Hypothesis 1 was that cryopreservation (-196 °C) of drone semen is more effective for long-term storage than at 16 °C. Our results show that after 1 yr of storage, frozen sperm viability was higher than at 16 °C, showing that cryopreservation is necessary to conserve semen. However, the cryoprotectant used for drone sperm freezing, dimethyl sulfoxide (DMSO), can harm the queen and reduce fertility after instrumental insemination. Hypothesis 2 was that centrifugation of cryopreserved semen to reduce DMSO prior to insemination optimize sperm quality. Our results indicate that centrifuging cryopreserved sperm to remove cryoprotectant does not affect queen survival, spermathecal sperm count, or sperm viability. Although these data do not indicate that centrifugation of frozen-thawed sperm improves queen health and fertility after instrumental insemination, we demonstrate that cryopreservation is achievable, and it is better for long-term sperm storage than above-freezing temperatures for duration of close to a year. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  6. Wolbachia in two populations of Melittobia digitata Dahms (Hymenoptera: Eulophidae)

    International Nuclear Information System (INIS)

    Copeland, Claudia S.; Sivinski, John

    2008-01-01

    We investigated two populations of Melittobia digitata Dahms, a gregarious parasitoid (primarily upon a wide range of solitary bees, wasps, and flies), in search of Wolbachia infection. The first population, from Xalapa, Mexico, was originally collected from and reared on Mexican fruit fly pupae, Anastrepha ludens Loew (Diptera: Tephritidae); the other, from Athens, Georgia, was collected from and reared on prepupae of mud dauber wasps, Trypoxylon politum Say (Hymenoptera: Crabronidae). PCR studies of the ITS2 region corroborated that both parasitoid populations were the same species; this potentially provides a useful molecular taxonomic profile since females of Melittobia species are superficially similar. Amplification of the Wolbachia surface protein gene (wsp) confirmed the presence of this endosymbiont in both populations. Sequencing revealed that the Wolbachia harbored in both populations exhibited a wsp belonging to a unique subgroup (denoted here as Dig) within the B-supergroup of known wsp genes. This new subgroup of wsp may either belong to a different strain of Wolbachia from those previously found to infect Melittobia or may be the result of a recombination event. In either case, known hosts of Wolbachia with a wsp of this subgroup are only distantly related taxonomically. Reasons are advanced as to why Melittobia - an easily reared and managed parasitoid - holds promise as an instructive model organism of Wolbachia infection amenable to the investigation of Wolbachia strains among its diverse hosts. (author)

  7. Wolbachia in two populations of Melittobia digitata Dahms (Hymenoptera: Eulophidae)

    Energy Technology Data Exchange (ETDEWEB)

    Copeland, Claudia S.; Sivinski, John [United States Dept. of Agriculture, Gainesville, FL (United States). Center for Medical, Agriculture and Veterinary Entomology]. E-mails: cclaudia@bioinf.uni-leipzig.de; john.sivinski@ars.usda.gov; Matthews, Robert W. [University of Georgia, Athens, GA (United States). Dept. of Entomology]. E-mail: rmatthew@uga.edu; Gonzalez, Jorge M. [Texas A and M Univ., College Station, TX (United States). Dept. of Entomology]. E-mail: jmgonzalez@neo.tamu.edu; Aluja, Martin [Instituto de Ecologia A.C., Veracruz (Mexico)]. E-mail: martin.aluja@inecol.edu.mx

    2008-11-15

    We investigated two populations of Melittobia digitata Dahms, a gregarious parasitoid (primarily upon a wide range of solitary bees, wasps, and flies), in search of Wolbachia infection. The first population, from Xalapa, Mexico, was originally collected from and reared on Mexican fruit fly pupae, Anastrepha ludens Loew (Diptera: Tephritidae); the other, from Athens, Georgia, was collected from and reared on prepupae of mud dauber wasps, Trypoxylon politum Say (Hymenoptera: Crabronidae). PCR studies of the ITS2 region corroborated that both parasitoid populations were the same species; this potentially provides a useful molecular taxonomic profile since females of Melittobia species are superficially similar. Amplification of the Wolbachia surface protein gene (wsp) confirmed the presence of this endosymbiont in both populations. Sequencing revealed that the Wolbachia harbored in both populations exhibited a wsp belonging to a unique subgroup (denoted here as Dig) within the B-supergroup of known wsp genes. This new subgroup of wsp may either belong to a different strain of Wolbachia from those previously found to infect Melittobia or may be the result of a recombination event. In either case, known hosts of Wolbachia with a wsp of this subgroup are only distantly related taxonomically. Reasons are advanced as to why Melittobia - an easily reared and managed parasitoid - holds promise as an instructive model organism of Wolbachia infection amenable to the investigation of Wolbachia strains among its diverse hosts. (author)

  8. Optimal management and productivity of Eucalyptus grandis on former phosphate mined and citrus lands in central and southern Florida: influence of genetics and spacing

    Science.gov (United States)

    Kyle W. Fabbro; Donald L. Rockwood

    2016-01-01

    Eucalyptus short rotation woody crops (SRWC) with superior genotypes are promising in central and south Florida due to their fast growth, freeze resilience, coppicing ability, and site tolerance. Four Eucalyptus grandis cultivars, E.nergy™ G1, G2, G3, and/or G5, were established in 2009 at varying planting densities on a...

  9. Standardization of a protocol to obtain genomic DNA for the quantification of 5mC in epicormics buds of Tectona grandis L.

    OpenAIRE

    Elisa Quiala; Luis Valledor; Rodrigo Hazbun; Raúl Barbón; Manuel de Feria; Maité Chávez

    2008-01-01

    The present investigation was carried out with the objective of defining an extraction and purification method that it provided deoxyribonucleic acid (DNA) appropriate to determine the percentage of 5mC in the genomic DNA of epicormics buds of Tectona grandis L. During the standardization of the protocol four methods were compared: 1 -classic based on saline shock solution with CTAB (hexadecil trimetil ammonium bromide), 2 - Kit of extraction of DNA plants DNeasy Plant Mini Kit (QIAGEN) accor...

  10. Stimulatory effect of eucalyptus essential oil on the germination and early growth of Eucalyptus grandis seedlings Efeito estimulante do óleo essencial de eucalipto na germinação e crescimento inicial de mudas de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Ricardo Bemfica Steffen

    2010-12-01

    Full Text Available

    The use of essential oil and extracts from plants is becoming an efficient alternative in the biostimulation on growth and protection of plants. The aim of this work was to evaluate the use of leaf essential oil of Eucalyptus grandis on the germination and the  development of Eucalyptus grandis seedlings in nursery conditions. The eucalyptus seeds were exposed to the concentrations of 0, 10, 25, 50, 75 and 100 μL L-1 of the essential oil in controlled conditions. The eucalyptus seedlings were sprayed with 0, 20, 30, 40, 50 and 60 μL L-1 of the essential oil per plant, at intervals of seven days. The effect of this application on the seedling development were analyzed after 30 and 60 days. The results show that the germination was significantly higher when the seeds were exposed to 25 μL L-1 of the essential oil. The application of essential oil in the concentration of 30 and 40 μL L-1 provided higher growth of the aerial part and of the roots in greenhouse conditions, being an effective alternative to biostimulation the vegetative growth of eucalyptus seedlings.

    doi: 10.4336/2010.pfb.30.63.199

    A utilização de extratos e óleos essenciais de plantas vem sendo uma alternativa eficiente no bioestímulo do crescimento e proteção vegetal. O trabalho teve por objetivo avaliar a utilização do óleo essencial extraído de folhas de Eucalyptus grandis sobre a germinação e o desenvolvimento inicial de mudas de eucalipto em condições controladas, em casa de vegetação. Sementes de eucalipto foram incubadas em  concentrações de 0, 10, 25, 50, 75 e 100 μL L-1 do óleo essencial em ambiente controlado.  Posteriormente, pulverizou-se plântulas de eucalipto com 0, 20, 30, 40, 50 e 60 μL L-1 do óleo essencial por planta, em intervalos de sete dias. Avaliou-se o efeito do óleo sobre o desenvolvimento vegetal aos 30 e 60 dias após o transplante. Os resultados evidenciam que a germinação foi significativamente maior

  11. Effects of Urban Waste and Mineral Fertilizers Applications on Eucalyptus grandis Growth and Soil Conditions Efeito da Aplicação de Lixo Urbano Compostado e de Adubos Minerais no Solo e na Produtividade de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Guilherme de Castro Andrade

    2011-03-01

    Full Text Available

    Forest plantations have been studied as a potential ecosystem able to absorb organic residues as fertilizers or soil conditioners. This study evaluate the effects of the application of composted organic waste and mineral fertilizers in a Eucalyptus grandis (W. Hill ex Maiden plantation with 86 months of age in Neossolo Quartzarênico. It was analyzed wood production, litter decomposition and the physical and chemical changes in the soil properties. It was observed that the municipal composted organic wastes resulted in an increase of 45.6% in wood volume, as compared to the control. It also increase the velocity of the litter
    decomposition on average of 28% and 16% for leaves and twigs respectively, increasing the pH level values and the availability of water in the soil (from 13% to 25% more available water in the first layer of the soil, 0 -10 cm. 
    As florestas têm sido estudadas como ecossistemas aptos à absorver resíduos orgânicos, utilizados como fertilizantes e condicionadores do solo. Este trabalho avaliou no Município de Itatinga, SP, em Neossolo Quartzarênico, os efeitos da aplicação de lixo urbano compostado e da adubação mineral em plantações de Eucalyptus grandis com 86 meses de idade. Foram analisadas as produtividades de madeira, a decomposição da serapilheira e as propriedades químicas e físicas do
    solo. Verificou-se que a aplicação de lixo urbano aumentou a produtividade de E. grandis com relação à testemunha, com um ganho de 45,6 % no volume cilíndrico de madeira. O solo com aplicação de lixo urbano apresentou um menor tempo de decomposição da serapilheira, em média com reduções de 28 % para folhas e de 16 % para galhos, com aumentos no pH e na água disponível no solo de 13 % a 25 %, na camada de 0 a 10 cm de profundidade, que podem justificar a maior
    produção de madeira obtida no final da rotação, em

  12. Selection of Beauveria bassiana sensu lato and Metarhizium anisopliae sensu lato isolates as microbial control agents against the boll weevil (Anthonomus grandis) in Argentina.

    Science.gov (United States)

    Nussenbaum, A L; Lecuona, R E

    2012-05-01

    The boll weevil (Anthonomus grandis) is the main pest of cotton in the Americas. The aim of this work was to evaluate isolates of the entomopathogenic fungi Beauveria bassiana sensu lato and Metarhizium anisopliae sensu lato virulent against A. grandis. Screening was performed to evaluate the pathogenicity of 28 isolates of M. anisopliae s.l. and 66 isolates of B. bassiana s.l. against boll weevil adults. To select the isolates, LC(50) values of the most virulent isolates were calculated, and compatibility between the fungi and insecticides was studied. In addition, the effects of these isolates on the feeding behavior of the adults were evaluated. Isolates Ma 50 and Ma 20 were the most virulent against A. grandis and their LC(50) values were 1.13×10(7) and 1.20×10(7) conidia/ml, respectively. In addition, these isolates were compatible with pyrethroid insecticides, but none with endosulfan. On the other hand, infected females reduced the damage caused by feeding on the cotton squares and their weight gain. This shows that entomopathogenic fungi cause mortality in the insects, but also these fungi could influence the feeding behavior of the females. In summary, these results indicate the possibility of the use of M. anisopliae s.l. as a microbiological control agent against boll weevils. Also, this species could be included in an Integrated Pest Management program. Copyright © 2012 Elsevier Inc. All rights reserved.

  13. Systematic chemical profiling of Citrus grandis 'Tomentosa' by ultra-fast liquid chromatography/diode-array detector/quadrupole time-of-flight tandem mass spectrometry.

    Science.gov (United States)

    Li, Pan-lin; Liu, Meng-hua; Hu, Jie-hui; Su, Wei-wei

    2014-03-01

    Citrus grandis 'Tomentosa', as the original plant of the traditional Chinese medicine "Huajuhong", has been used as antitussive and expectorant in clinic for thousands of years. The fruit epicarp and whole fruit of this plant were both literarily recorded and commonly used. In the present study, an ultra-fast liquid chromatography coupled with diode-array detection and quadrupole/time-of-flight mass spectrometry (UFLC-DAD-Q-TOF-MS/MS) based chemical profiling method was developed for rapid holistic quality evaluation of C. grandis 'Tomentosa', which laid basis for chemical comparison of two medicinal parts. As a result, forty-eight constituents, mainly belonging to flavonoids and coumarins, were unambiguously identified by comparison with reference standards and/or tentatively characterized by elucidating UV spectra, quasi-molecular ions and fragment ions referring to information available in literature. Both of the epicarp and whole fruit samples were rich in flavonoids and coumarins, but major flavonoids contents in whole fruit were significantly higher than in epicarp (P<0.5). The proposed method could be useful in quality control and standardization of C. grandis 'Tomentosa' raw materials and its products. Results obtained in this study will provide a basis for quality assessment and further study in vivo. Copyright © 2013 Elsevier B.V. All rights reserved.

  14. RESISTÊNCIA BIOLÓGICA DA MADEIRA TRATADA DE Eucalyptus grandis E Eucalyptus cloeziana A FUNGOS APODRECEDORES EM ENSAIOS DE LABORATÓRIO

    Directory of Open Access Journals (Sweden)

    Magnos Alan Vivian

    2015-01-01

    Full Text Available The present study aimed to evaluate the biological resistance of treated wood from Eucalyptus grandis and Eucalyptus cloeziana under the action of biodeteriorative organisms in laboratory testing. Thus, we used trees of Eucalyptus grandis and Eucalyptus cloeziana, both 16 years old, which was converted into planks and subjected to preservative treatment in an autoclave with chromate copper arsenate (CCA. Then, it was made the specimens for the conduct of accelerated decay test, as recommended by ASTM. From the results, it was observed for the fungus Trametes versicolor that the preservative treatment was effective in reducing the biological degradation of the wood of the two species, with reduced mass loss in 35.17 and 82.31% for wood Eucalyptus grandis and Eucalyptus cloeziana, respectively, as for the fungus Gloeophyllum trabeum mass loss was reduced by 6.79 and 96.65%, compared to the control. Based on the conditions of realization of the present study, it was observed that preservative treatment with CCA is effective in the increasing the biological resistance of the wood under the action of fungi Trametes versicolor and Gloeophyllum trabeum.

  15. Filtration and clearance rates of Anadara grandis juveniles (Pelecypoda, Arcidae with different temperatures and suspended matter concentrations

    Directory of Open Access Journals (Sweden)

    Anselmo Miranda-Baeza

    2006-09-01

    Full Text Available The mangrove cockle Anadara grandis (Broderip and Sowerby, 1829 is a potential candidate for aquaculture and for bioremediation of aquaculture effluents in the tropical and subtropical coastal areas of the eastern Pacific Ocean. Laboratory-produced spat are available, but there is no information on their responses to the range of environmental conditions to which they might be subject during the growth cycle. The aim of this study was to evaluate the filtration and clearance rates of A. grandis spat (shell length 9.50±0.37 mm with a food concentration (7.5 mg∙l-1 at four different temperatures (22, 25, 28 and 31 ºC, with pH = 7.5±0.2 and O2 concentration of 6.4±0.5 mg·l-1; experiment one; and with a temperature (25 °C and five concentrations of suspended matter (from 7.5 to 29 mg·l-1 and pH and O2 values of 7.9±0.2 and 6.8± 0.4 mg·l-1; experiment two. Filtration and clearance rates were highest at 25 ºC and significantly different (p.05. In the second experiment filtration increased according to the amount of food available, but there were no significant differences (p>.05 between 7.5 and 11 mg·l-1 and from 22.4 to 29 mg·l-1. The trend was similar for clearance, and in this case significant differences were found (pLa almeja Anadara grandis (Broderip and Sowerby, 1829 es un candidato potencial para la acuicultura y la biorremediación de efluentes acuícolas en las áreas costeras tropicales y subtropicales del océano Pacífico oriental. Se dispone de semilla producida en laboratorio, sin embargo no hay información sobre sus respuestas a los intervalos de las condiciones ambientales a las cuales puede estar sujeta durante el periodo de crecimiento. El objetivo de este estudio fue evaluar las tasas de filtración y de clarificación de semilla de A. grandis (largo de la concha= 9.50±0.37 mm con una concentración de alimento (7.5 mg∙l-1 y cuatro diferentes temperaturas (22, 25, 28 y 31 °C con pH= 7.5±0.2, concentración de O

  16. Efeito do tempo de armazenamento de minestacas no enraizamento de clones de Eucalyptus grandis x E. urophylla Effect of storage time of minicuttings on the rooting of Eucalyptus grandis x E. urophylla clones

    Directory of Open Access Journals (Sweden)

    Patrícia Bueno Goulart

    2008-08-01

    Full Text Available Este estudo teve como objetivo avaliar o efeito do tempo de armazenamento de miniestacas no processo de enraizamento de quatro clones de Eucalyptus grandis x E. urophylla. As miniestacas foram coletadas em minijardim clonal conduzido em sistema de hidroponia em canaletas. O delineamento experimental utilizado foi em blocos ao acaso, em arranjo fatorial 6 x 4, constituído de seis tratamentos (estaqueamento após a coleta e estaqueamento após o armazenamento em câmara fria por 1, 2, 4, 8 e 16 dias e quatro clones, em quatro repetições e parcelas compostas de 16 plantas/repetição. Foram realizadas avaliações do porcentual de enraizamento e crescimento das miniestacas enraizadas em casa de vegetação, casa de sombra e a pleno sol. Concluiu-se que o plantio das miniestacas logo após a coleta no minijardim clonal foi o que proporcionou melhor resposta ao enraizamento nos quatro clones estudados, sendo observado efeito negativo do armazenamento dos propágulos, mesmo quando realizado por curto período de tempo.The objective of the present work was to evaluate the effect of the storage time of minicuttings on the rooting process of four clones of Eucalyptus grandis x E. urophylla. The minicuttings were collected in a clonal minigarden carried on in a hydroponic system in small gutters. The experimental design used was of random plots, in a factorial arrangement 6 x 4, constituted by six treatments (cutting after collection, cutting after storage in cold chamber per 1, 2, 4, 8 and 16 days and four clones, in four repetitions and plots of 16 plants per repetition. Evaluations of the rooting percentage and growth of the rooted minicuttings in a greenhouse, a shade house and in the open sun were made. It was concluded that planting the minicuttings right after the collection in the clonal miniorchard had the best rooting response for the four clones studied and also that there was a negative effect of the storage, even when stored for a short

  17. Qualidade de mudas clonais de Eucalyptus urophylla x E. grandis em função do substrato Quality of Eucalyptus urophylla x E. grandis cuttings as a function of substrate

    Directory of Open Access Journals (Sweden)

    Richardson B. G. da Silva

    2012-01-01

    Full Text Available Considerando a crescente demanda por mudas florestais e a escassez de matérias-primas dos substratos utilizados para o crescimento das plantas, faz-se necessário avaliar novos componentes e formulações que assegurem a qualidade das mudas. Nesse experimento foram estudados o desenvolvimento e a qualidade de mudas clonais de Eucalyptus urophylla x E. grandis, provenientes de miniestacas, em função de nove composições de substrato produzidos a partir de vermiculita granulometria fina, casca de arroz carbonizada e fibra de coco em tubetes de 50 mL. Foram avaliados as propriedades físicas dos substratos, o desenvolvimento morfológico e a qualidade do sistema radicular das mudas, aos 90 dias após o estaqueamento. Os substratos com maior porosidade total promovem maior qualidade do sistema radicular o que, consequentemente, resulta em mudas com maior diâmetro, massa seca aérea e radicular e Índice de Qualidade de Dickson. Outros valores para as características físicas dos substratos, diferentes dos citados na literatura, também podem ser considerados adequados.Considering the increasing demand for seedlings of plants for aforestation and the scarcity of raw materials for the substrates used for plant growth, it is necessary to evaluate new components and formulations that ensure the quality of seedlings. In this experiment the development and quality of production of Eucalyptus urophylla x E. grandis seedlings was studied through vegetative propagation, as a function of nine compositions of substrate, produced from fine-grained vermiculite, carbonized rice chaff and coconut fiber in hard plastic tubes of 50 mL as containers. The physical properties of substrates and morphological development and the quality of the root system of seedlings were evaluated at 90 days after staking. The substrates with higher total porosity promotes better quality of the root system, which consequently results in cuttings with larger diameter, shoot and

  18. Calcium and potassium silicates and the growth of Eucalyptus grandis seedlings Aplicação de silicatos de cálcio e de potássio e o crescimento de mudas de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Itamar Antonio Bognola

    2011-06-01

    Full Text Available

    The use of silicate is a practice that has been frequently adopted in annual crops, although few studies have been conducted in order to verify its efficiency in forest tree species. The main objective of this research was to evaluate the effect of calcium and potassium silicates in the growth of seedlings of Eucalyptus grandis. The experiment was conducted in a greenhouse, in Alambari county, State of São Paulo. A completely randomized experimental design was adopted, with eight treatments, three replications and 25 plants per plot, maintained at the field capacity moisture level. Seedlings were planted in plastic tubes using local substrate, basic fertilization and silicates. Diameter, height and fresh and dry biomass of the seedlings were determined 150 days after silicates treatments were applied. The use of silicates either in the substrate or through foliar application, in the production of eucalypt seedlings has proven to be inadequate when the substrate presents a balanced basic composition in terms of nutrients and pH.

    doi: 10.4336/2011.pfb.31.66.83

    O uso de silicato é uma prática que vem sendo adotada com frequência em culturas anuais, muito embora poucos trabalhos tenham sido realizados com intuito de verificar sua eficiência em espécies florestais arbóreas. O objetivo principal desta pesquisa foi avaliar o efeito da aplicação de silicatos de cálcio e de potássio no crescimento de mudas de Eucalyptus grandis. O experimento foi conduzido em casa de vegetação, no Município de Alambari, SP. O delineamento usado foi inteiramente ao acaso, com oito tratamentos, três repetições e 25 plantas por parcela, sendo mantidas na umidade de capacidade de campo. As mudas foram plantadas em tubetes de plástico, utilizando substrato local, com adubação básica e silicatos. Diâmetro de colo, altura e biomassa verde e seca das mudas foram determinados 150 dias após a

  19. Consumo Foliar de Eucalyptus spp. por Acromyrmex disciger (Mayr, 1887 (Hymenoptera: Formicidae

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    Wagner Calixto Morais

    2011-07-01

    Abstract. The productive potential of forest stands is reduced by pest occurrence among other factors. In Brazil, leaf-cutting ants are the most severe eucalypt pests. Acromyrmex disciger (Mayr is prevalent in the south east Brazil. However, scarce information about its potential damage for Eucalyptus forests is available. This work deals to quantifying the eucalypt leaf-consumption by such specie of leaf-cutting ant. Fresh leaves were taken from trees of Eucalyptus grandis W. Hill ex Maiden, Eucalyptus urophylla ST Blake, and hybrid E. urophylla x E. grandis and served to different colonies of A. disciger, during 24 hours period, over eight different times. Leaf-consumption was calculated throughout fresh weights of leaves, before and after ants foraging. Each colony of A. disciger consumed 38.8 ± 3.2 g e 22.0 ± 2.3 g of eucalypt leaves, per day.

  20. Epidemiological study of the prevalence of allergic reactions to Hymenoptera in a rural population in the Mediterranean area.

    Science.gov (United States)

    Fernandez, J; Blanca, M; Soriano, V; Sanchez, J; Juarez, C

    1999-08-01

    Systemic allergic reactions to Hymenoptera venom occur in a percentage that varies from 0.4 to 3.3%. Epidemiological studies indicate that from 15 to 25% of the general population can be sensitized to different Hymenoptera venom as well as the fact that the degree of exposure may be related to the prevalence found in those studies. The objective of this study was to evaluate the prevalence of insect sting allergy and the venom sensitization in a rural population to three Hymenoptera previously found in the area: Polistes dominulus (Pd), Vespula germanica (Vg) and honey bee (Hb). A rural community located in the south-east of Spain, close to the Mediterranean Sea, was selected since the stinging Hymenoptera having been previously identified. A random sample of 310 subjects from the village census was studied. A questionnaire and a serum sample were obtained from every patient. The evaluation was conducted by a family doctor, who focused on the reactions to Hymenoptera sting, age, sex, occupation, atopia, previous Hymenoptera sting, stinging insect, interval to last sting and average stings per year. RAST to Hymenoptera venoms were also determined. The prevalence of systemic reactions was 2.3% (57.6% of them had a positive RAST). Large local reactions were found in 26.4% (only 28.5% of them had a positive RAST). Asymptomatic sensitization (positive RAST) was observed in 16.4% of subjects without reaction. Only a weak correlation between subjects with less than 3 years' interval to last sting exposure and positive RAST results was noted, whether they presented with a clinical reaction or not (P < 0.05). The prevalence of systemic sting reactions in our rural community is higher than other general populations in the same Mediterranean area, and similar to other rural populations studied. The degree of exposure influences not only the prevalence found but also the detection of specific serum IgE.

  1. Características tecnológicas da madeira de árvores matrizes de Eucalyptus grandis.

    Directory of Open Access Journals (Sweden)

    Clovis Roberto Haselein

    2010-08-01

    Full Text Available O objetivo do presente trabalho foi caracterizar árvores matrizes de Eucalyptus grandis em relação a características tecnológicas da madeira. Foram selecionadas 63 árvores matrizes, pertencentes a um povoamento comercial localizado no litoral norte do Rio Grande do Sul. As árvores foram abatidas e, com base nos dados dendrométricos, calculou-se o volume comercial com e sem casca, o fator de forma, a conicidade e a relação altura/diâmetro. Quanto à madeira, foi analisada a massa específica básica, os percentuais volumétricos de cerne, alburno e casca, as rachaduras de topo das toras e das tábuas, os empenamentos e os defeitos visuais das tábuas, tais como nós e bolsa de resina. A massa específica básica, cuja média inclui a madeira dessa espécie entre as moderadamente leves a pesadas, mostrou uma tendência decrescente entre o DAP e 25% da altura comercial e, a partir daí, crescente até 100% da mesma. O percentual volumétrico de cerne apresentou valores médios crescentes desde a base até 25% da altura comercial e, a partir daí, diminuiu até 100% da altura comercial, sendo que a média geral foi de 75,7%. O comprimento médio das rachaduras de topo em tábuas de Eucalyptus grandis foi de 63%. A alta porcentagem de rachaduras de topo foi atribuída à posição de retirada das tábuas, próxima à medula. Já a variabilidade das rachaduras de topo encontrada para as toras (CV=60% possibilita a seleção de árvores com menor tendência em apresentar esse defeito.

  2. Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of Eucalyptus grandis and E. urophylla

    Science.gov (United States)

    2012-01-01

    Background F1 hybrid clones of Eucalyptus grandis and E. urophylla are widely grown for pulp and paper production in tropical and subtropical regions. Volume growth and wood quality are priority objectives in Eucalyptus tree improvement. The molecular basis of quantitative variation and trait expression in eucalypt hybrids, however, remains largely unknown. The recent availability of a draft genome sequence (http://www.phytozome.net) and genome-wide genotyping platforms, combined with high levels of genetic variation and high linkage disequilibrium in hybrid crosses, greatly facilitate the detection of quantitative trait loci (QTLs) as well as underlying candidate genes for growth and wood property traits. In this study, we used Diversity Arrays Technology markers to assess the genetic architecture of volume growth (diameter at breast height, DBH) and wood basic density in four-year-old progeny of an interspecific backcross pedigree of E. grandis and E. urophylla. In addition, we used Illumina RNA-Seq expression profiling in the E. urophylla backcross family to identify cis- and trans-acting polymorphisms (eQTLs) affecting transcript abundance of genes underlying QTLs for wood basic density. Results A total of five QTLs for DBH and 12 for wood basic density were identified in the two backcross families. Individual QTLs for DBH and wood basic density explained 3.1 to 12.2% of phenotypic variation. Candidate genes underlying QTLs for wood basic density on linkage groups 8 and 9 were found to share trans-acting eQTLs located on linkage groups 4 and 10, which in turn coincided with QTLs for wood basic density suggesting that these QTLs represent segregating components of an underlying transcriptional network. Conclusion This is the first demonstration of the use of next-generation expression profiling to quantify transcript abundance in a segregating tree population and identify candidate genes potentially affecting wood property variation. The QTLs identified in this

  3. Nesting biology of Centris (Hemisiella tarsata Smith (Hymenoptera, Apidae, Centridini

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    Cândida M. L. Aguiar

    2004-09-01

    Full Text Available Nests of Centris tarsata Smith, 1874 were obtained from trap-nests in areas of dry semi-deciduous forest (Baixa Grande and caatinga (Ipirá, in the State of Bahia. Nesting occurred in bamboo canes and in tubes of black cardboard with 5.8 cm (= small tube and 10.5 cm (= large tube in length and 0.6 and 0.8 cm in diameter, respectively. In both areas C. tarsata nested during the wet season producing four generations in Baixa Grande and three generations in Ipirá. The immatures of one generation underwent diapause at both sites. The bees constructed their nests with a mixture of sand and oil. In general, the cells were elongated and arranged in linear series with its opening pointing towards the nest entrance. Completed nests had two to three cells in small tubes, one to seven cells in large tubes, and two to 13 cells in bamboo canes. The nest plug resembled an uncompleted cell and was externally covered with oil. The cells were provisioned with pollen, oil, and nectar. Nests were parasitized by Mesocheira bicolor (Fabricius, 1804 (Hymenoptera: Apidae and other not identify bee species.Ninhos de Centris tarsata Smith, 1874 foram obtidos através da utilização de ninhos-armadilha, em áreas de floresta estacional semi-decídua (Baixa Grande e de caatinga (Ipirá, no Estado da Bahia. A nidificação ocorreu em gomos de bambus e em tubos de cartolina preta, estes com comprimentos de 5,8 cm (= tubos pequenos e 10,5 cm (= tubos grandes, e diâmetro de 0,6 e 0,8 cm, respectivamente. Em ambas as áreas C. tarsata nidificou durante a estação úmida, produzindo quatro gerações anuais em Baixa Grande e três em Ipirá. Os imaturos de uma das gerações passaram por diapausa em ambos os locais. As abelhas construíram seus ninhos com uma mistura de areia e óleo. Em geral, as células foram alongadas e arranjadas em série linear, com sua abertura dirigida para a entrada do ninho. Os ninhos completados tinham de duas a três células nos tubos pequenos

  4. Fertility signals in the bumblebee Bombus terrestris (Hymenoptera: Apidae)

    Science.gov (United States)

    Sramkova, A.; Schulz, C.; Twele, R.; Francke, W.; Ayasse, M.

    2008-06-01

    In eusocial Hymenoptera, queen control over workers is probably inseparable from the mechanism of queen recognition. In primitively eusocial bumblebees ( Bombus), worker reproduction is controlled not only by the presence or absence of a dominant queen but also by other dominant workers. Furthermore, it was shown that the queen dominance is maintained by pheromonal cues. We investigated whether there is a similar odor signal released by egg-laying queens and workers that may have a function as a fertility signal. We collected cuticular surface extracts from nest-searching and breeding Bombus terrestris queens and workers that were characterized by their ovarian stages. In chemical analyses, we identified 61 compounds consisting of aldehydes, alkanes, alkenes, and fatty acid esters. Nest-searching queens and all groups of breeding females differed significantly in their odor bouquets. Furthermore, workers before the competition point (time point of colony development where workers start to develop ovaries and lay eggs) differed largely from queens and all other groups of workers. Breeding queens showed a unique bouquet of chemical compounds and certain queen-specific compounds, and the differences toward workers decrease with an increasing development of the workers’ ovaries, hinting the presence of a reliable fertility signal. Among the worker groups, the smallest differences were found after the competition point. Egg-laying females contained higher total amounts of chemical compounds and of relative proportions of wax-type esters and aldehydes than nest-searching queens and workers before the competition point. Therefore, these compounds may have a function as a fertility signal present in queens and workers.

  5. Simplification of intradermal skin testing in Hymenoptera venom allergic children.

    Science.gov (United States)

    Cichocka-Jarosz, Ewa; Stobiecki, Marcin; Brzyski, Piotr; Rogatko, Iwona; Nittner-Marszalska, Marita; Sztefko, Krystyna; Czarnobilska, Ewa; Lis, Grzegorz; Nowak-Węgrzyn, Anna

    2017-03-01

    The direct comparison between children and adults with Hymenoptera venom anaphylaxis (HVA) has never been extensively reported. Severe HVA with IgE-documented mechanism is the recommendation for venom immunotherapy, regardless of age. To determine the differences in the basic diagnostic profile between children and adults with severe HVA and its practical implications. We reviewed the medical records of 91 children and 121 adults. Bee venom allergy was exposure dependent, regardless of age (P bee venom allergic group, specific IgE levels were significantly higher in children (29.5 kU A /L; interquartile range, 11.30-66.30 kU A /L) compared with adults (5.10 kU A /L; interquartile range, 2.03-8.30 kU A /L) (P venom were higher in bee venom allergic children compared with the wasp venom allergic children (P venom. At concentrations lower than 0.1 μg/mL, 16% of wasp venom allergic children and 39% of bee venom allergic children had positive intradermal test results. The median tryptase level was significantly higher in adults than in children for the entire study group (P = .002), as well as in bee (P = .002) and wasp venom allergic groups (P = .049). The basic diagnostic profile in severe HVA reactors is age dependent. Lower skin test reactivity to culprit venom in children may have practical application in starting the intradermal test procedure with higher venom concentrations. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  6. Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae

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    Vladimir Gokhman

    2011-08-01

    Full Text Available Drosophila melanogaster Meigen, 1830 has served as a model insect for over a century. Sequencing of the 11 additional Drosophila Fallen, 1823 species marks substantial progress in comparative genomics of this genus. By comparison, practically nothing is known about the genome size or genome sequences of parasitic wasps of Drosophila. Here, we present the first comparative analysis of genome size and karyotype structures of Drosophila parasitoids of the Leptopilina Förster, 1869 and Ganaspis Förster, 1869 species. The gametic genome size of Ganaspis xanthopoda (Ashmead, 1896 is larger than those of the three Leptopilina species studied. The genome sizes of all parasitic wasps studied here are also larger than those known for all Drosophila species. Surprisingly, genome sizes of these Drosophila parasitoids exceed the average value known for all previously studied Hymenoptera. The haploid chromosome number of both Leptopilina heterotoma (Thomson, 1862 and L. victoriae Nordlander, 1980 is ten. A chromosomal fusion appears to have produced a distinct karyotype for L. boulardi (Barbotin, Carton et Keiner-Pillault, 1979 (n = 9, whose genome size is smaller than that of wasps of the L. heterotoma clade. Like L. boulardi, the haploid chromosome number for G. xanthopoda is also nine. Our studies reveal a positive, but non linear, correlation between the genome size and total chromosome length in Drosophila parasitoids. These Drosophila parasitoids differ widely in their host range, and utilize different infection strategies to overcome host defense. Their comparative genomics, in relation to their exceptionally well-characterized hosts, will prove to be valuable for understanding the molecular basis of the host-parasite arms race and how such mechanisms shape the genetic structures of insect communities.

  7. Thermoperiodism Synchronizes Emergence in the Alfalfa Leafcutting Bee (Hymenoptera: Megachilidae).

    Science.gov (United States)

    Yocum, George D; Rinehart, Joseph P; Yocum, Ian S; Kemp, William P; Greenlee, Kendra J

    2016-02-01

    Alfalfa seed production in the northwestern United States and western Canada is heavily dependent upon the pollinating services of Megachile rotundata (F.) (Hymenoptera: Megachilidae). M. rotundata females nest in cavities either naturally occurring or in artificial nesting blocks. Because of the physical nature of the nest, M. rotundata brood may have limited to no exposure to photoperiodic cues in order to regulate important circadian functions. Therefore, various thermoperiod regimes were used to characterize the possible role of thermoperiodism in synchronizing M. rotundata adult emergence. Adult emergence was monitored using a microprocessor-controlled event logger. Incubating bees under constant 29°C and darkness resulted in an arhythmic adult emergence pattern. Exposing developing M. rotundata to a thermoperiod synchronized emergence to the beginning of the thermophase and decreased the total number of days required for all adults to emerge. The amplitude of the thermoperiod regulated the timing of peak emergence in relationship to the increase in temperature. A thermoperiod amplitude of only 2°C was sufficient to synchronize peak adult emergence to take place during the rise in temperature. Increasing the amplitude of the thermoperiod to 4 or 8°C caused a positively correlated shift in peak emergence to later in the thermophase. Brood stored under constant 29°C and darkness for different durations (May or June early in the growing season or July or August late in the growing season) or under a fluctuating thermal regime (base temperature of 6°C and daily 1-h pulse of 20°C until September or November) maintained their capacity for entraining emergence timing by thermoperiodism. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  8. Migration and dispersal of Anthonomus grandis (Coleoptera: Curculionidae in South America Migración y dispersión de Anthonomus grandis (Coleoptera: Curculionidae en América del Sur

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    Teodoro Stadler

    2007-12-01

    Full Text Available This study discusses the dispersal of Anthonomus grandis Boheman, the cotton boll weevil, in Argentina, Brazil, Paraguay and Bolivia, exploring the ecological and physiological factors that have made the dispersal and establishment of this insect in South America so successful. The boll weevil's phenotypic plasticity is represented by its flexible developmental time, its multivoltine life cycle with several overlapping generations, its capacity to feed on pollen from diverse botanical families as well as from non pollen food sources and its ability to migrate and disperse aided by winds. These characteristics make it a key pest for cotton. Probable overwintering «hot spots» for the boll weevil were identified in Misiones-Argentina, where large numbers of prediapausing weevils concentrate after arrival from newly harvested cotton fields in Paraguay, probably attracted by citrus orchards volatiles. The boll weevil's facultative quiescence is always relative to environmental adverse conditions. This suggests that overwintering in the boll weevil can be defined as «oligopause», an intermediate form of diapause. Since its introduction to Brazil in 1983, until 2006, it has spread southwest at an average of 61 km year-1 towards Argentina. However, it took the boll weevil approximately ten years to move 250 km between Paraguay and the main cotton growing area in Argentina. This slower progress is probably due to the actions taken by the Argentine government through the boll weevil eradication program. The arrival of the boll weevil at the cotton cropping areas in Paraguay and Argentina reinforces the fact that the boll weevil should finally be included in an integrated cotton pest management program jointly with other major cotton pests.El presente estudio sobre la dispersión de Anthonomus grandis Boheman, el picudo del algodonero, en Argentina, Brasil, Paraguay y Bolivia, explora las características ecológicas y fisiológicas que han permitido a

  9. Pontoscolex corethrurus (Annelida: Oligochaeta indicador de la calidad del suelo en sitios de Eucalyptus grandis (Myrtacea con manejo tumba y quema Pontoscolex corethrurus (Annelidae: Oligochaeta soil quality indicator in Eucalyptus grandis (Myrtacea sites with slash and burn management

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    Sheila Uribe

    2012-12-01

    Full Text Available La presencia de oligoquetos en los ecosistemas puede indicar fertilidad del suelo, ya que estos organismos transportan, mezclan y entierran los residuos vegetales de la superficie al interior del suelo. Se caracterizó la comunidad de oligoquetos bajo sitios con diferentes periodos de establecimiento y manejo de plantaciones de Eucalyptus grandis, sin vegetación (SV, con cinco años en producción (Euc y vegetación secundaria con 15 años (Acah que han pasado por el proceso de tumba y quema en suelos de Acrisol en Huimanguillo, Tabasco; y se analizaron las propiedades físico-químicas del suelo (D.A., humedad, textura, pH, Ntot, MO, P, K, CIC. La recolecta de lombrices se realizó al finalizar las lluvias (agosto-octubre 2007. Se muestreó en tres parcelas con seis réplicas en cada una. Se encontró que los suelos tenían pH de 3.0-4.5 en los primeros 30cm de profundidad. Los contenidos de materia orgánica (MO y nitrógeno total (Ntot fueron significativamente menores en los sitios SV (6-8% y 0.19-0.22% respectivamente que en Euc y Acah (MO=9-11%; el Ntot=0.27-0.33%. La especie Pontoscolex corethrurus domino en toda el área, presentando mayores densidades y biomasas en Euc (164.4ind/m² y 36.8g/m² respectivamente y Acah (138.7ind/m² y 19.1g/m² respectivamente, mientras que en SV sus poblaciones fueron reducidas en un 80%. Se encontró que el sistema Acah sigue presentando rasgos de un sistema perturbado, al no recuperar fácilmente la diversidad de oligoquetos y las concentraciones de nutrientes disponibles en el sueloSoil burning has been used in agricultural and forestry systems as a fundamental technique to clean the land and add some nutrients to the soil. In addition, earthworms are known to promote various soil functions since they contribute to aeration and organic matter and nutrients availability to other soil organisms. This study evaluated the effects of tropical forest crops management with presence-absence of Eucalyptus

  10. Energy from briquettes produced from remains of urban solid residues and wood of Eucalyptus grandis; Energia de briquetes produzidos com rejeitos de residuos solidos urbanos e madeira de Eucalyptus grandis

    Energy Technology Data Exchange (ETDEWEB)

    Goncalves, Jose E.; Leao, Alcides L. [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Dept. de Ciencias Ambientais], emails: evaristo@fca.unesp.br, alcidesleao@fca.unesp.br; Sartori, Maria M.P. [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Bauru, SP (Brazil). Dept. de Engenharia de Producao], email: msartori@btu.flash.tv.br

    2009-07-01

    The incentive for consumption and production in large quantity in modern society generates enormous amounts of urban solid residues in the form of municipal solid waste (MSW). With the intention of reducing these residues of the municipal waste tips and to generate energy, briquettes with mixtures of MSW and residues of Eucalyptus grandis were produced. The briquettes were manufactured with 0, 5, 10, 15, 20 and 25% of MSW in the mixture with wood waste and 12% of moisture content. The analyzed parameters used to choose the best treatments were combustion analysis versus ash content, mechanical strength and energy content. The briquettes up to 10% of MSW showed low resistance, and above 15% showed large increase in ash content. Therefore, the treatment that fulfilled the requirements for combustion versus ash content and mechanical resistance was of at least 15% of MSW, since the source of the ash is unidentified. Considering the net energy content, the best treatment was 25% of MSW, with 17,175 kJ kg{sup -1}. Nevertheless, it is strongly advised that further studies related to gas emissions are necessary. (author)

  11. Utilização de madeiras de Eucalyptus grandis E Eucalyptus dunnii para produção de painéis de partículas orientadas – OSB

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    Setsuo Iwakiri

    2008-01-01

    Full Text Available This study was developed to evaluate the feasibility of OSB manufacturing using woods of Eucalyptus grandis and Eucalyptus dunnii. Boards with nominal density of 0,70 g/cm³ and 1,0 g/cm³ were manufactured in laboratory, using 100% of wood particles from Pinus taeda, Eucalyptus grandis and Eucalyptus dunnii, and mixtures of 50% of Pinus taeda in the internal layer of the board, with 50% of Eucalyptus grandis and 50% of Eucalyptus dunnii. The boards of Eucalyptus grandis with density of 0,70 g/cm³, as standard board density, showed the values of properties compatible with the requirements of the Canadian and European Standards and also in relation of boards manufactured from Pinus taeda. The results of the mechanical properties showed an increase in the MOE and MOR in static bending with the increase in the board density, opening the possibility to use the high density OSB for applications requiring higher strength. The results of this research indicate that wood of Eucalyptus grandis can be used as alternative specie to OSB manufacturing in the Brazil.

  12. Los Chalcidoidea (Hymenoptera asociados con agallas de Cinípidos (Hymenoptera, Cynipidae en la Comunidad de Madrid

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    Gómez, J. F.

    2006-05-01

    Full Text Available Information about the chalcid wasp parasitoid community (Hymenoptera, Chalcidoidea associated with galls of Cynipidae in Madrid (Spain is compiled and updated. Studied material includes more than 1000 published and unpublished records from samplings in 80 sites in the Madrid region carried out over twenty three years by the research team. A check-list of 121 species, 19 of them provisionally identified, from 6 families of Chalcidoidea is provided as follows: 26 Eurytomidae, 27 Torymidae, 9 Ormyridae, 33 Pteromalidae, 9 Eupelmidae and 17 Eulophidae. Ormyrus rufimanus Mayr, 1904 and Idiomacromerus semiaeneus (Szelenyi, 1957 are recorded for the first time in the Iberian Peninsula. For each family and genus of Chalcidoidea data are given on biology, diversity and distribution in Comunidad de Madrid. The composition of the chalcid wasp parasitoid community associated with gall wasps in Comunidad de Madrid is discussed and compared to the same community data from the Iberian Peninsula and the Western Palaearctic. Species from the Iberian community of chalcid parasitoids that are missing from Madrid region, exclusive species and potentially present species are also commented upon. Finally two appendices are presented as follows: a list of the Chalcidoidea species reared from 73 different galls made by 71 cynipid species from Madrid with associated parasitoid records, and a final appendix with full record data of all studied material, including information on the sampling localities with X, Y georeferenced coordinates, host galls and host plants.

    Se compila y actualiza la información existente sobre la comunidad parasitoide de Chalcidoidea, asociada a agallas de cinípidos (Hymenoptera, Cynipidae en la Comunidad de Madrid. Los datos corresponden a más de 1000 registros, tanto de datos publicados, como inéditos, correspondientes a colectas en 80 localidades de Madrid efectuadas por el equipo investigador a lo largo de 23 a

  13. Efeito dos cofatores hidroquinona, prolina e triptofano no enraizamento de miniestacas de clones de Eucalyptus grandis x E. urophylla

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    Patrícia Bueno Goulart

    2011-10-01

    Full Text Available Este estudo teve como objetivo avaliar a eficiência dos cofatores hidroquinona, prolina e triptofano, associados ao regulador de crescimento AIB (ácido indolbutírico na propagação vegetativa pelo enraizamento de miniestacas de quatro clones de Eucalyptus grandis x E. urophylla. As miniestacas foram coletadas no minijardim clonal realizado em sistema de hidroponia em canaletas. O delineamento experimental utilizado foi o inteiramente casualizado, constituído de quatro concentrações para cada cofator e quatro clones, em quatro repetições e parcelas compostas de 16 plantas/repetição. Foram realizadas avaliações na casa de vegetação, casa de sombra e ao sol nas miniestacas enraizadas. Concluiu-se que o triptofano (0,8 mg L-1 e a hidroquinona (0,2 a 0,4 mg L-1, aplicados associados ao AIB, melhoraram o enraizamento em três clones dos quatro clones avaliados; enquanto a prolina (0,2 a 0,4 mg L-1, aplicada associada ao AIB, promoveu melhoria expressiva no enraizamento das miniestacas nos quatros clones estudados, evidenciando ser entre os cofatores testados o mais eficiente.

  14. Receptor chirality and behavioral specificity of the boll weevil,Anthonomus grandis Boh. (Coleoptera: Curculionidae), for its pheromone, (+)-grandisol.

    Science.gov (United States)

    Dickens, J C; Mori, K

    1989-02-01

    Electrophysiological recordings from antennal olfactory receptors and field behavioral experiments showed both male and female boll weevils,Anthonomus grandis Boh. (Coleoptera: Curculionidae), to respond specifically to (+)-grandisol, an enantiomer of compound I of the boll weevil aggregation pheromone. Single-cell recordings revealed antennal olfactory neurons in both male and female weevils keyed to (+)-grandisol. Electroantennograms in response to serial dilutions of the grandisol enaniiomers showed a threshold 100 to 1000 times lower for (+)-grandisol relative to its antipode. In field behavioral experiments, both sexes were significantly more attracted to (+)-grandisol in combination with the three other pheromone components than the combination with (-)-grandisol. When (-)-grandisol was placed with the (+)-enantiomer at equal dosages, a slight although statistically insignificant inhibition occurred. Subsequent field tests showed that the low level of attraction exhibited by (-)-grandisol in combination with the other three pheromone components could be attributed to the other three components alone. These results are in contrast with an earlier study, which found (-)-grandisol to be as attractive as the (+)-enantiomer.

  15. Efficacy of the organic-certified insecticide Diatect II against the boll weevil (Anthonomus grandis) in cotton.

    Science.gov (United States)

    Sappington, Thomas W

    2002-10-01

    The efficacy of the organic insecticide Diatect II against boll weevil (Anthonomus grandis Boheman) in cotton (Gossypium hirsutum L) in the Lower Rio Grande Valley of Texas were assessed in small-plot field trials and greenhouse cage tests using azinphos-methyl treatments as a standard for comparison. Plastic sheets were placed in the furrows of the treated plots to retrieve boll weevils which dropped from the plants after being killed by the insecticides. Samples of live weevils taken by a tractor-mounted vacuum sampler revealed a modest, but significant, reduction in boll weevil populations in Diatect II plots. However, samples of dead weevils indicated that this reduction was due to movement of weevils out of the plots rather than to mortality. This interpretation is supported by greenhouse cage studies, where mortality in Diatect II treated cages was no greater than that in untreated control cages. The effects of insecticide treatments in small plots can be confounded easily and quickly by interplot movement of target insects. Although the relative effects of various compounds can usually be assessed by sampling the populations in plots soon after treatment, the best measure of efficacy is obtained by directly sampling insects that have died in the plot. This parameter is insulated from the effects of interplot movement, unless the toxicant is slow to immobilize the target insect. Taken together, our results indicate little efficacy by Diatect II against boll weevil under our test conditions.

  16. Between-season attraction of cotton boll weevil, Anthonomus grandis Boh. (Coleoptera: Curculionidae adults by its aggregation pheromone

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    Fernandes Wedson Desidério

    2001-01-01

    Full Text Available The present study was undertaken to investigate the attractiveness of boll weevil adults by its aggregation pheromone under winter field conditions. Two experimental fields were utilized at "Casa Branca", SP, Brazil. For each one, three areas were established near the refuge vegetation, sparated 500 m from each other. Each area was divided in three sub-areas or blocks of 100 m² to receive pheromone applications (2.5 g per block. In addition to the pre-application counting, five additional evaluations were carried out after the pheromone applicaton. Ten randomized sampling points per block were considered in each evaluation process. A. grandis adults responded immediately to the pheromone applications, and were captured for 14 days . The highest level of attractiveness was observed 24 hours after application. The application of the boll weevil aggregation pheromone during winter could increase the predation by natural enemies, due to the increase of prey availability. Chemical control can be recommended 24 hours after pheromone applications in small plots as a between-season strategy for the suppression of boll weevil adults.

  17. Supercritical Carbon Dioxide Extraction of Flavonoids from Pomelo (Citrus grandis (L.) Osbeck) Peel and Their Antioxidant Activity

    Science.gov (United States)

    He, Jin-Zhe; Shao, Ping; Liu, Jian-Hua; Ru, Qiao-Mei

    2012-01-01

    Supercritical carbon dioxide (SC-CO2) extraction of flavonoids from pomelo (Citrus grandis (L.) Osbeck) peel and their antioxidant activity were investigated. Box-Behnken design combined with response surface methodology was employed to maximize the extraction yield of flavonoids. Correlation analysis of the mathematical-regression model indicated that a quadratic polynomial model could be used to optimize the SC-CO2 extraction of flavonoids. The optimal conditions for obtaining the highest extraction yield of flavonoids from pomelo peel were a temperature of 80 °C, a pressure of 39 MPa and a static extraction time of 49 min in the presence of 85% ethanol as modifier. Under these conditions, the experimental yield was 2.37%, which matched positively with the value predicted by the model. Furthermore, flavonoids obtained by SC-CO2 extraction showed a higher scavenging activity on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals than those obtained by conventional solvent extraction (CSE). Therefore, SC-CO2 extraction can be considered as a suitable technique for the obtainment of flavonoids from pomelo peel. PMID:23202938

  18. Dissection of the Mechanism for Compatible and Incompatible Graft Combinations of Citrus grandis (L. Osbeck (‘Hongmian Miyou’

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    Wen He

    2018-02-01

    Full Text Available ‘Hongmian miyou’ (Citrus grandis L. Osbeck is mutated from ‘Guanxi miyou’, with a different spongy layer coloration. Trifoliate orange (Poncirus trifoliata is widely used as rootstocks in ‘Guanxi miyou’ grafting, whereas ‘Hongmian miyou’ is incompatible with available trifoliate orange rootstocks. To explore the reasons for the etiolation of leaves of ‘Hongmian miyou’/trifoliate orange, anatomical differences among different graft unions, gene expression profiles, and auxin levels of scion were investigated in this study. A histological assay indicated that there was no significant difference in anatomical structure between the compatible and incompatible combinations. A total of 1950 significant differentially-expressed genes (DEGs were identified and analyzed. The Kyoto Encyclopedia of Genes and Genomes (KEGG pathway enrichment analysis revealed that genes involved in carbohydrate metabolism, energy metabolism, amino acid metabolism, and plant hormone signal transduction were significantly enriched. Moreover, the expression of nine genes in the auxin pathway were upregulated and three were downregulated in compatible combinations compared with those in the incompatible group. Further experiments verified that indole-3-acetic acid (IAA content increases in the compatible graft combination, which suggests that IAA might promote graft compatibility.

  19. INFLUENCE OF ANATOMICAL FEATURES AND EXTRACTIVES CONTENT WOOD OF Eucalyptus grandis W. Hill ex Maiden IN QUALITY BONDING

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    Vanessa Cristina do Sacramento Albino

    2012-12-01

    Full Text Available http://dx.doi.org/10.5902/198050987561The study aimed to anatomically characterize and to quantify the total extractives in different positions of the log of Eucalyptus grandis W. Hill ex Maiden with 18 years of age and to assess their influence on the shear strength and percentage of wood failure in adhesive bonded joints with thermosetting resorcinol-formaldehyde. Histological slides were prepared for an anatomical study and determined the total extractive content. The preparation of bonded joints was made according to ASTM D 2339-98(2000. It was used the correlation coefficient of Pearson, 1% probability by t test to evaluate the correlation between the anatomical and extractives content with the quality of the glued joints. It was found that there was a correlation between the shear strength with the following characteristics: vessel diameter, wall width, length and width of the fiber, the width of the radius and total extractives content. For the percentage of wood failure, there was a correlation between this with the following characteristics: the frequency of vessels, thickness of the heat of fiber, and high frequency of lightning. Both the radial direction as in the longitudinal direction of the logs there was change in the values found for the measurement of the anatomical elements, the total amount of extractives, the shear test strength and the percentage of wood failure.

  20. Adsorption equilibrium and kinetic studies of crystal violet and naphthol green on torreya-grandis-skin-based activated carbon

    International Nuclear Information System (INIS)

    Dai, Wei; Yu, Huijing; Ma, Na; Yan, Xiaoyang

    2015-01-01

    A new type of activated carbon, torreya-grandis-skin-based activated carbon (TAC), has been used to remove the harmful dyes (cationic dye crystal violet (CV) and anionic dye naphthol green (NG)) from contaminated water via batch adsorption. The effects of solution pH, adsorption time and temperature were studied. The Langmuir and Freundlich adsorption models were used to describe the equilibrium isotherm and isotherm constant calculation. It was found that the maximum equilibrium adsorption capacities were 292mg/g and 545mg/g for CV and NG, respectively. Adsorption kinetics was verified by pseudo-first-order, pseudo-second-order and intra-particle diffusion kinetic models. Results indicated that the rate of dye adsorption followed pseudo-second-order kinetic model for the initial dye concentration range studied. Temperature-dependent adsorption behavior of CV and NG shows that the adsorption is spontaneous and endothermic, accompanying an entropy increase. This work indicates that TAC could be employed as a low-cost alternative for the removal of the textile dyes from effluents

  1. Climatic-Induced Shifts in the Distribution of Teak ( Tectona grandis) in Tropical Asia: Implications for Forest Management and Planning

    Science.gov (United States)

    Deb, Jiban Chandra; Phinn, Stuart; Butt, Nathalie; McAlpine, Clive A.

    2017-09-01

    Modelling the future suitable climate space for tree species has become a widely used tool for forest management planning under global climate change. Teak ( Tectona grandis) is one of the most valuable tropical hardwood species in the international timber market, and natural teak forests are distributed from India through Myanmar, Laos and Thailand. The extents of teak forests are shrinking due to deforestation and the local impacts of global climate change. However, the direct impacts of climate changes on the continental-scale distributions of native and non-native teak have not been examined. In this study, we developed a species distribution model for teak across its entire native distribution in tropical Asia, and its non-native distribution in Bangladesh. We used presence-only records of trees and twelve environmental variables that were most representative for current teak distributions in South and Southeast Asia. MaxEnt (maximum entropy) models were used to model the distributions of teak under current and future climate scenarios. We found that land use/land cover change and elevation were the two most important variables explaining the current and future distributions of native and non-native teak in tropical Asia. Changes in annual precipitation, precipitation seasonality and annual mean actual evapotranspiration may result in shifts in the distributions of teak across tropical Asia. We discuss the implications for the conservation of critical teak habitats, forest management planning, and risks of biological invasion that may occur due to its cultivation in non-native ranges.

  2. RELATIONSHIP BETWEEN CLIMATE VARIABLES, TRUNK GROWTH RATE AND WOOD DENSITY OF Eucalyptus grandis W. Mill ex Maiden TREES

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    Carlos Roberto Sette Jr

    2016-04-01

    Full Text Available ABSTRACT Climatic conditions stimulates the cambial activity of plants, and cause significant changes in trunk diameter growth and wood characteristics. The objective of this study was to evaluate the influence of climate variables in the diameter growth rate of the stem and the wood density of Eucalyptus grandis trees in different classes of the basal area. A total of 25 Eucalyptus trees at 22 months of age were selected according to the basal area distribution. Dendrometer bands were installed at the height of 1.30 meters (DBH to monitor the diameter growth every 14 days, for 26 months. After measuring growth, the trees were felled and wood discs were removed at the DBH level to determine the radial density profile through x-ray microdensitometry and then re-scale the average values every 14 days. Climatic variables for the monitoring period were obtained and grouped every 14 days. The effect of the climate variables was determined by maximum and minimum growth periods in assessing trunk growth. These growth periods were related with precipitation, average temperature and relative air humidity. The re-scaled wood density values, calculated using the radial growth of the tree trunks measured accurately with steel dendrometers, enabled the determination of the relationship of small changes in wood density and the effect of the climatic variations and growth rate of eucalyptus tree trunks. A high sensitivity of the wood density to variation in precipitation levels was found.

  3. ESTABELECIMENTO A CAMPO DE MUDAS DE Eucalyptus grandis MICORRIZADAS COM Pisolithus microcarpus (UFSC Pt 116 EM SOLO ARENOSO

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    Andréa Hentz de Mello

    2009-01-01

    Full Text Available The aim of this work was to evaluate the survival and the initial growth of mycorrhizated eucalypts with Pisolithus microcarpus (UFSC Pt 116 ectomycorrhizal fungus, after its transplant to area subject to the arenization process in São Francisco de Assis, RS. The area was divided into four blocks, each one with four treatments (fertile turf with and without mycorrhizae, Quartzarenic Neosoil with and without mycorrhizae. Each parcel was composed of 16 seedlings arranged in four lines in the spacing of 1,5 m x 1,5 m, totalizing in each block 64 seedlings. 90 days after the planting in the field, the eucaliptus seedlings produced in turf with fungus in the fertile substratum presented a survival rate of 100 %, whereas for those produced in fertile turf without fingi, the survival rate was 92 %. The seedlings produced in the Quartzarenic Neosoil with and without mycorrhizae had a survival rate varying around 98 and 89 %, respectively. The produced seedlings with turf and fungus showed significant differences in height and stem diameter. This study showed that the Eucalyptus grandis seedlings produced in substratum fertile turf and inoculated with the Pisolithus microcarpus (UFSC Pt 116 isolated may maintain good development and establishment in the field.

  4. Comparative interrogation of the developing xylem transcriptomes of two wood-forming species: Populus trichocarpa and Eucalyptus grandis.

    Science.gov (United States)

    Hefer, Charles A; Mizrachi, Eshchar; Myburg, Alexander A; Douglas, Carl J; Mansfield, Shawn D

    2015-06-01

    Wood formation is a complex developmental process governed by genetic and environmental stimuli. Populus and Eucalyptus are fast-growing, high-yielding tree genera that represent ecologically and economically important species suitable for generating significant lignocellulosic biomass. Comparative analysis of the developing xylem and leaf transcriptomes of Populus trichocarpa and Eucalyptus grandis together with phylogenetic analyses identified clusters of homologous genes preferentially expressed during xylem formation in both species. A conserved set of 336 single gene pairs showed highly similar xylem preferential expression patterns, as well as evidence of high functional constraint. Individual members of multi-gene orthologous clusters known to be involved in secondary cell wall biosynthesis also showed conserved xylem expression profiles. However, species-specific expression as well as opposite (xylem versus leaf) expression patterns observed for a subset of genes suggest subtle differences in the transcriptional regulation important for xylem development in each species. Using sequence similarity and gene expression status, we identified functional homologs likely to be involved in xylem developmental and biosynthetic processes in Populus and Eucalyptus. Our study suggests that, while genes involved in secondary cell wall biosynthesis show high levels of gene expression conservation, differential regulation of some xylem development genes may give rise to unique xylem properties. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

  5. Arthropods of Rose Atoll with special reference to ants and Pulvinaria Urbicola Scales (Hempitera Coccidae) on Pisonia Grandis trees

    Science.gov (United States)

    Banko, Paul C.; Peck, Robert W.; Pendleton, Frank; Schmaedick, Mark; Ernsberger, Kelsie

    2014-01-01

    Rose Atoll, at the eastern end of the Samoan Archipelago, is a small but important refuge for seabirds, shorebirds, and sea turtles. While the vertebrate community is relatively well-studied, the terrestrial arthropod fauna, and its role in ecosystem function, are poorly known. Arthropods may be influencing the decline of Pisonia grandis, an ecologically important tree that once dominated the 6.6 ha of land on Rose Atoll. Reasons for the decline are not fully understood but a facultative relationship between two invasive arthropods, the soft scale Pulvinaria urbicola and ants, likely has contributed to tree death. The primary objectives of this study were to systematically survey the terrestrial arthropod fauna and identify ant species that tend scales on Pisonia. Using an array of standard arthropod collecting techniques, at least 73 species from 20 orders were identified, including nine ant species. Of the ants collected, only Tetramorium bicarinatum and T. simillimum were observed tending scales on Pisonia. No known natural enemies of Pulvinaria scales were found, suggesting little predation on scale populations. Treatment of Pisonia with the systemic insecticide imidacloprid failed to eliminate Pulvinaria scales, although short-term suppression apparently occurred. The arthropod fauna of Rose Atoll is dominated by exotic species that likely have a significant impact on the structure and function of the island’s ecosystem.

  6. Influência do vermicomposto na produção de mudas de Eucalyptus grandis Hill ex Maiden

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    Mauro Valdir Schumacher

    2001-01-01

    Full Text Available Considering the large area of planted forest with species of the genus Eucalyptus and its uses for multiple purpose, its cultivation has an economic importance in Brazil. The use of substratum with good physic-chemical characteristics is an important point for the quality of the produced seedlings. The objective of this work was to evaluate the effect of different doses of vermicompost in containers with capacity of about 50 cm3 of substratum. The current study was conducted in a greenhouse with Eucalyptus grandis from January to April in 1997. The substratum used for seedlings production was the soil from horizon A (0-20 cm of São Pedro Unit Mapping (yellow red Podzolic with different vermicompost quantities (0; 5.0; 10.0; 15.0 and 20.0 cm3. The vermicompost was produced from red-worm from California (Eisenia foetida Savigny with cow dung. After 100 days, the results of development for the same seedlings were different depending on doses of vermicompost. It was verified that quantities over 15.0 cm3 (30% of vermicompost per container are not recommended for the seedling production.

  7. Toxicity of Chlorpyrifos and Dimethoate to the Ciliate Urostyla grandis, with Notes on Their Effects on Cell Ultrastructure

    Science.gov (United States)

    Mu, Weijie; Warren, Alan; Pan, Xuming; Ying, Chen

    2018-06-01

    Chlorpyrifos and dimethoate are overused agricultural pesticides that can trigger trophic cascades, resulting in toxicity to both terrestrial and aquatic organisms as well as altered ecosystems. In previous studies, substantial attention has been given to the effects of pesticides on vertebrate species and, to a lesser extent, species of zooplankton. The present study was designed to show that the fission time effective concentration in ciliates is a potential aquatic detection index for environmental monitoring. The ciliate Urostyla grandis was treated with doses of chlorpyrifos and dimethoate. After exposed to the pesticides, the LC 50 ( i.e., concentration that killed 50% of the ciliate cells within 24 h) values were 0.029 mg L-1 for chlorpyrifos and 0.0685 mg L-1 for dimethoate. The fission time effective concentrations after 168 h of exposure were 0.0075-0.0093 mg L-1 for chlorpyrifos and 0.2640-0.2750 mg L-1 for dimethoate. These results show that the fission time effective concentration is lower than the LC 50 value in ciliates, indicating that fission time effective concentration is more suitable than the LC 50 value for environmental monitoring using ciliates. The effects of chlorpyrifos and dimethoate on ciliate cell ultrastructures included agglutination of chromatin in the macronucleus, protruded and discontinuous macronuclear and micronuclear membranes, loss of integrity of mitochondrial membranes and contents, and abscission and deformation of the adoral zone of membranelles.

  8. SPECTRORADIOMETRY IN THE VISIBLE AND NEAR INFRARED REGION ON A STAND OF Eucalyptus grandis Hill ex-Maiden

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    Catize Brandelero

    2012-03-01

    Full Text Available http://dx.doi.org/10.5902/198050985093Reflectance readings in border and inner tree leaves in a Eucalyptus grandis stand, in São Pedro das Missões, Rio Grande do Sul state, were analyzed in the regions of the visible electromagnetic spectrum and the nearby infrared, by using spectrum radiometry. The area was divided in two parts: border and center stands. In order to collect the material, the crown was divided in three parts (superior, medium and inferior, so that it would be possible to differentiate the positions of leaf collections in each area. Three trees were sampled in each area, adding up to six trees, for each tree, 60 isolated leaves were collected, 20 in each position. The reflectance readings were carried out through FieldSpec®3 spectrum radiometer and the final results were segmented in the visible and nearby infrared spectral bands. The statistical analysis was made on the basis of several tests, among them Tukey HSD test, in order to compare the averages of the visible region, which, according to ANOVA, present significant differences. It is concluded that the collecting indicating class of leaves for the spectrum radiometric analysis in the visible region are preferably the 5 one (tree in the center, reading in the medium part and #3 one (border tree, reading superior part.

  9. Supercritical Carbon Dioxide Extraction of Flavonoids from Pomelo (Citrus grandis (L. Osbeck Peel and Their Antioxidant Activity

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    Qiao-Mei Ru

    2012-10-01

    Full Text Available Supercritical carbon dioxide (SC-CO2 extraction of flavonoids from pomelo (Citrus grandis (L. Osbeck peel and their antioxidant activity were investigated. Box-Behnken design combined with response surface methodology was employed to maximize the extraction yield of flavonoids. Correlation analysis of the mathematical-regression model indicated that a quadratic polynomial model could be used to optimize the SC-CO2 extraction of flavonoids. The optimal conditions for obtaining the highest extraction yield of flavonoids from pomelo peel were a temperature of 80 °C, a pressure of 39 MPa and a static extraction time of 49 min in the presence of 85% ethanol as modifier. Under these conditions, the experimental yield was 2.37%, which matched positively with the value predicted by the model. Furthermore, flavonoids obtained by SC-CO2 extraction showed a higher scavenging activity on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS radicals than those obtained by conventional solvent extraction (CSE. Therefore, SC-CO2 extraction can be considered as a suitable technique for the obtainment of flavonoids from pomelo peel.

  10. Partial cytochrome b sequences for six Hymenoptera of the eastern United States.

    Science.gov (United States)

    Collins, A M; Gardner, L M

    2001-01-01

    Mitochondrial DNA (mtDNA) haplotypes have been commonly used to determine honeybee subspecies relationships. To see if these markers would also be useful for comparisons of other Hymenoptera, we collected workers of six local species: Vespa crabro, the European hornet; Bombus impatiens, a bumblebee; Vespula germanica, the German yellow jacket; Polistes fuscatus, a paper wasp; Halictus ligatus, an alkali bee; and an unspecified Megachile, a leafcutting bee. MtDNA was isolated and digested with six endonucleases (AvaI, BglII, EcoRI, HindIII, HinfI, XbaI). The digested DNA was electrophoresed and visualized on agarose gels with comparison to a standard fragment marker and similarly treated honeybee mtDNA. The fragments obtained were also purified and sequenced. Phylogenetic relationships between six wasp and bee species, Apis mellifera, and several other similar aculeate Hymenoptera were determined. Newly defined DNA sequences were posted to GenBank (AF281169-AF281174).

  11. Ichneumonidae (Hymenoptera) parasitóides de larvas de Lepidoptera associadas a Croton floribundus Spreng (Euphorbiaceae)

    OpenAIRE

    Fernandes, Luciana Bueno dos Reis; Dias Filho, Manoel Martins; Fernandes, Marcelo Adorna; Penteado-Dias, Angelica Maria

    2010-01-01

    Parasitoids of the family Ichneumonidae (Hymenoptera) were obtained during an inventory of Lepidoptera larvae caught feeding in the wild on Croton floribundus (Euphorbiaceae). The Lepidoptera larvae were collected from host plants along trails inside three preserved forest areas in the Brazilian state of São Paulo. Fifteen different species of Ichneumonidae belonging to five subfamilies (Banchinae, Campopleginae, Cremastinae, Mesochorinae and Metopiinae) were obtained. Seven species of Ichneu...

  12. A Gynandromorph and Teratological Case in Spilomicrus sp. (Hymenoptera, Diaprioidea, Diapriidae

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    Emerson Comério

    2015-12-01

    Resumo. Este estudo relata a ocorrência de antenas com características femininas e masculinas em um exemplar fêmea de Spilomicrus sp. (Hymenoptera, Diaprioidea, Diapriidae coletado no Parque Estadual Intervales, Ribeirão Grande, São Paulo, Brasil, assim como malformações em uma das antenas, que são aqui descritas e ilustradas.

  13. Morphology and function of the ovipositor mechanism in Ceraphronoidea (Hymenoptera, Apocrita

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    Andrew Ernst

    2013-08-01

    Full Text Available The ovipositor of apocritan Hymenoptera is an invaluable source of phylogenetically relevant characters, and our understanding of its functional morphology stands to enlighten us about parasitoid life history strategies. Although Ceraphronoidea is one of the most commonly collected Hymenoptera taxa with considerable economic importance, our knowledge about their natural history and phylogenetic relationships, both to other apocritan lineages and within the superfamily itself, is limited. As a first step towards revealing ceraphronoid natural diversity we describe the skeletomuscular system of the ceraphronoid ovipositor for the first time. Dissections and Confocal Laser Scanning Microscopy 3D media files were used to visualize the ovipositor complex and to develop character concepts. Morphological structures were described in natural language and then translated into a character-character state format, whose terminology was linked to phenotype-relevant ontologies. Four unique anatomical phenotypes were revealed: 1. The first valvifer (gonangulum of the genus Trassedia is composed of two articulating sclerites, a condition present only in a few basal insect taxa. The bipartition of the first valvifer in Trassedia is most likely secondary and might allow more rapid oviposition. 2. Ceraphronoids, unlike other Hymenoptera, lack the retractor muscle of the terebra; instead the egg laying device is retracted by the seventh sternite. 3. Also unlike other Hymenoptera, the cordate apodeme and the anterior flange of the second valvifer are fused and compose one ridge that serves as the site of attachment for the dorsal and ventral T9-second valvifer muscles. Overall, the ceraphronoid ovipositor system is highly variable and can be described by discrete, distinguishable character states. However, these differences, despite their discrete nature, do not reflect the present classification of the superfamily and might represent parallelisms driven by host

  14. Identification of microsatellite markers for a worldwide distibuted, highly invasive ant species Tapinoma melanocephalum (Hymenoptera: Formicidiae)

    Czech Academy of Sciences Publication Activity Database

    Zima, Jan; Lebrasseur, O.; Borovanská, Michaela; Janda, Milan

    2016-01-01

    Roč. 113, JUNE 03 (2016), s. 409-414 E-ISSN 1802-8829 R&D Projects: GA ČR GAP505/12/2467 Grant - others:Marie Curie Fellowship(CZ) PIOFGA2009-25448 Institutional support: RVO:60077344 Keywords : Hymenoptera * Formicidae * Tapinoma melanocephalum Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.167, year: 2016 http://www.eje.cz/pdfs/eje/2016/01/53.pdf

  15. Accelerated evolution of mitochondrial but not nuclear genomes of Hymenoptera: new evidence from crabronid wasps.

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    Martin Kaltenpoth

    Full Text Available Mitochondrial genes in animals are especially useful as molecular markers for the reconstruction of phylogenies among closely related taxa, due to the generally high substitution rates. Several insect orders, notably Hymenoptera and Phthiraptera, show exceptionally high rates of mitochondrial molecular evolution, which has been attributed to the parasitic lifestyle of current or ancestral members of these taxa. Parasitism has been hypothesized to entail frequent population bottlenecks that increase rates of molecular evolution by reducing the efficiency of purifying selection. This effect should result in elevated substitution rates of both nuclear and mitochondrial genes, but to date no extensive comparative study has tested this hypothesis in insects. Here we report the mitochondrial genome of a crabronid wasp, the European beewolf (Philanthus triangulum, Hymenoptera, Crabronidae, and we use it to compare evolutionary rates among the four largest holometabolous insect orders (Coleoptera, Diptera, Hymenoptera, Lepidoptera based on phylogenies reconstructed with whole mitochondrial genomes as well as four single-copy nuclear genes (18S rRNA, arginine kinase, wingless, phosphoenolpyruvate carboxykinase. The mt-genome of P. triangulum is 16,029 bp in size with a mean A+T content of 83.6%, and it encodes the 37 genes typically found in arthropod mt genomes (13 protein-coding, 22 tRNA, and two rRNA genes. Five translocations of tRNA genes were discovered relative to the putative ancestral genome arrangement in insects, and the unusual start codon TTG was predicted for cox2. Phylogenetic analyses revealed significantly longer branches leading to the apocritan Hymenoptera as well as the Orussoidea, to a lesser extent the Cephoidea, and, possibly, the Tenthredinoidea than any of the other holometabolous insect orders for all mitochondrial but none of the four nuclear genes tested. Thus, our results suggest that the ancestral parasitic lifestyle of

  16. Phylogeny and population genetic structure of ant genus Acropyga (Hymenoptera: Formicidae) in Papua New Guinea

    Czech Academy of Sciences Publication Activity Database

    Janda, Milan; Matos Maravi, Pavel F.; Borovanská, Michaela; Zima, Jan; Youngerman, E.; Pierce, N. E.

    2016-01-01

    Roč. 30, č. 1 (2016), s. 28-40 ISSN 1445-5226 R&D Projects: GA ČR GAP505/12/2467 Grant - others:Marie Curie Fellowship(CZ) PIOFGA2009-25448; Operational Program Research and Development for Innovations(CZ) CZ.1.05/3.2.00/08.0144 Institutional support: RVO:60077344 Keywords : Acropyga * Hymenoptera * Papua New Guinea Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.172, year: 2016

  17. Meteorus arizonensis Muesebeck, 1923 (Hymenoptera: Braconidae): nuevo registro para México

    OpenAIRE

    Gutiérrez-Ramírez, A.; Robles-Bermúdez, A.; Cambero-Campos, J.; Coronado-Blanco, J. M.

    2015-01-01

    Braconidae is one of the more diverse families of Hymenoptera with almost 20,000 species worldwide. Meteorus has 316 species and is almost cosmopolitan. Previously, seven species of this genus have been recorded from Mexico. In this note, Meteorus arizo-nensis Muesebeck is recorded for the first time for the country, obtained from the fall armyworm, Spodoptera frugiperda (J.E. Smith), with material from Santa Maria del Oro, locality in the State of Nayarit.

  18. Parasitoidism of Chalcidid wasps (Hymenoptera, Chalcididae on Philornis sp. (Diptera, Muscidae

    Directory of Open Access Journals (Sweden)

    M. S. Couri

    Full Text Available Philornis Meinert larvae are known as parasites of birds, with coprophagous, semi-hematophagous or hematophagous habits. Biological data of the larvae of the fifty described species are still scarcely known. Here we describe some aspects of the parasitism of a species of Philornis on Thalurania glaucopis Gmelin (Trochilidae and record two species of Chalcididae (Hymenoptera parasitoids, Conura annulifera (Walker, 1864 and Brachymeria podagrica (Fabricius, 1787, reared from Philornis puparia.

  19. Effect of entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae

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    Michele Potrich

    2018-01-01

    Full Text Available This study aimed to evaluate the effect of commercially used entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae. Four bioassays were performed: 1 pulverized entomopathogens on A. mellifera; 2 entomopathogens sprayed on a smooth surface; 3 entomopathogens sprayed on soy leaves; and 4 entomopathogens mixed with candy paste (sugar syrup. Five treatments were prepared: sterile distilled water (control, distilled water sterilized with Tween® 80 (0.01%, and the commercial entomopathogens Metarhizium anisopliae E9 (1.0 × 109 conidia mL−1, Beauveria bassiana PL63 (1.0 × 108 conidia mL−1 and Bacillus thuringiensis var. kurstaki HD-1 (3.0 × 108 spores mL−1. Each treatment consisted of five repetitions, with 20 workers per repetition, which were stored in a plastic box and, later, in a biological oxygen demand (B.O.D. incubator (27 ± 2 °C, RH of 60% ± 10%, 12-h photophase. The mortality of the workers was evaluated from 1 h to 240 h, and the data were analyzed using Bayesian inference. The workers killed by the ingestion of candy paste contaminated with the pathogens (products were randomly separated and selected for the removal of the midgut. Each midgut was fixed in Bouin's solution and prepared for histology. B. bassiana was verified to reduce the survival of A. mellifera workers in all bioassays. Moreover, M. anisopliae reduced the survival of A. mellifera workers directly sprayed, on a smooth surface and mixed with candy. B. thuringiensis reduced A. mellifera survival on a smooth surface and mixed with candy paste. However, its effects were lower than that observed by B. bassiana. The treatments with the biological products did not induce morphometric alterations in the midgut of A. mellifera. Keywords: Bayesian statistics, Entomopathogenic fungi, Entomopathogenic bacteria, Honeybee, Selectivity

  20. PARASITISM OF BOLL WEEVIL (Anthonomus grandis IN FLOWER BUDS OF COTTON PLANT, IN THE MUNICIPAL DISTRICT OF GOIÂNIA-GO PARASITISMO DO BICUDO DO ALGODOEIRO (Anthonomus grandis EM BOTÕES FLORAIS DO ALGODOEIRO, NO MUNICÍPIO DE GOIÂNIA-GO

    Directory of Open Access Journals (Sweden)

    Paulo Marçal Fernandes

    2007-09-01

    Full Text Available

    This work studied the indexes of parasitism of A. grandis in floral buttons of the cotton plants, collected in the soil and in the plants, in an area not treat with insecticides, located in the School of Agronomy of the Universidade Federal de Goiás, municipal district of Goiânia-GO. Floral buttons were collected with and without sign of oviparousness of the beaked ones. They presented larger parasitism occurrence in those collected in the soil. The parasites were identified as: Chelonus sp. (Microchelonus, Bracon sp. and Pteromalidae.

    KEY-WORDS: Insecta; parasitism; cotton plant; Anthonomus grandis.

    Estudou-se o índice de parasitismo de A. grandis em botões florais de algodoeiro coletados no solo e nas plantas, em uma área não tratada com inseticidas, localizada na Escola de Agronomia da Universidade Federal de Goiás, no município de Goiânia (GO. Foram coletados botões florais com e sem puncturas de oviposição dos bicudos. Verificou-se um maior parasitismo nos botões florais coletados no solo. Os parasitóides foram identificados como Chelonus sp. (Microchelonus, Bracon sp. e Pteromalidae.

    PALAVRAS-CHAVE: Insecta; parasitismo; algodoeiro; Anthonomus grandis.

  1. Efeito da hidrólise ácida dos taninos de Eucalyptus grandis w. hill ex Maiden nas propriedades dos adesivos tânicos Effect of the acid hydrolyses of Eucalyptus grandis w. hill ex Maiden tannins in the properties of the tannic adhesives

    Directory of Open Access Journals (Sweden)

    Angélica de Cássia Oliveira Carneiro

    2009-08-01

    Full Text Available Este trabalho teve como objetivo avaliar o efeito da hidrólise ácida sobre as propriedades e resistência ao cisalhamento dos adesivos de taninos, extraídos a partir da casca de Eucalyptus grandis W. Hill ex Maiden. Os adesivos foram sintetizados com taninos hidrolisados, empregando-se quatro valores de pH, três tempos de reação e 10% de formaldeído em relação à massa seca de taninos. Foram produzidas 96 juntas coladas, constituídas de duas lâminas de madeira de Eucalyptus grandis. As resistências ao cisalhamento e falha na madeira foram determinadas de acordo com a norma ASTM D 2339-93. Concluiu-se que a hidrólise ácida dos taninos reduziu a viscosidade dos adesivos e aumentou a resistência ao cisalhamento na linha de cola. De modo geral, as juntas coladas apresentaram baixo percentual de falha na madeira.The objective of this research was to evaluate the effect of the acid hydrolysis of Eucalyptus grandis W. Hill ex Maiden bark tannins on the properties of tannic adhesives. Adhesives were synthesized with tannins hydrolyzed at four pH values, three reaction times and 10% of formaldehyde based on the tannin dry weight. Ninety-six glued joints were prepared with Eucalyptus grandis thin boards. Shear resistance and wood failure percentage were determined according to the ASTM D 2339-93 standards. It was concluded that tannin hydrolysis decreased adhesive viscosity and increased the glue line shear resistance. However, it was observed a low percentage of wood failure.

  2. Energia de briquetes produzidos com rejeitos de resíduos sólidos urbanos e madeira de Eucalyptus grandis Energy from briquettes produced from remains of urban solid residues and wood of Eucalyptus grandis

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    José E. Gonçalves

    2009-10-01

    Full Text Available O incentivo ao consumo e à produção em grande quantidade na sociedade atual gera, exageros de resíduos sólidos urbanos que, em alguns casos, podem ser utilizados para a geração de energia. Neste sentido e visando reduzir os resíduos dos aterros municipais e gerar energia, buscou-se produzir briquetes com mistura de rejeitos de resíduos sólidos urbanos (RRSU e resíduos de madeira de Eucalyptus grandis. Os briquetes foram fabricados com 0, 5, 10, 15, 20 e 25% de RRSU na mistura com resíduos madeireiros contendo 12% de umidade. Os parâmetros analisados para a escolha da melhor mistura, foram: análise de combustibilidade x cinzas, resistência e energia utilizável. Os briquetes com até 10% de RRSU se mostraram com baixa resistência e os acima de 15% apresentaram grande aumento no teor de cinzas; portanto, os que melhor atenderam aos requisitos combustibilidade x cinzas e resistência mecânica, foram aqueles com 15% de RRSU, pois não se conhece a procedência das cinzas. Considerando-se a energia utilizável, o briquete com 25% de RRSU é o que apresenta maior poder calorífico útil na ordem de 17.175 kJ kg-1 motivo pelo qual se indica a produção de briquetes com adição RRSU; ressalta-se, porém, a necessidade de estudos sobre a emissão de gases.The incentive for consumption and production in large quantity in modern society generates enormous amounts of urban solid residues in the form of municipal solid waste (MSW. With the intention of reducing these residues of the municipal waste tips and to generate energy, briquettes with mixtures of MSW and residues of Eucalyptus grandis were produced. The briquettes were manufactured with 0, 5, 10, 15, 20 and 25% of MSW in the mixture with wood waste and 12% of moisture content. The analyzed parameters used to choose the best treatments were combustion analysis versus ash content, mechanical strength and energy content. The briquettes up to 10% of MSW showed low resistance, and above

  3. Caracterização bromatológica de oito linhagens de Lentinula edodes (Shiitake cultivadas em toras de Eucalyptus grandis Bromatological characterization of Lentinula edodes strains (Shiitake grown on Eucalyptus grandis logs

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    Meire Cristina Nogueira de Andrade

    2008-12-01

    Full Text Available O Lentinula edodes é um alimento de qualidade elevada, sendo rico em proteínas, vitaminas e sais minerais e pobre em calorias e gorduras. No entanto, seu valor nutricional varia em função da linhagem cultivada, do processamento após a colheita, do estágio de desenvolvimento do basidioma e do substrato utilizado. Assim, este trabalho teve como objetivo caracterizar bromatologicamente os basidiomas de oito linhagens de L. edodes (LE-95/01, LE-95/02, LE-95/07, LE-96/17, LE-96/18, LE-98/47, LE-98/55 e LE-96/13, inteiros ou em partes (estípete e píleo, produzidos em toras de E. grandis. Verificou-se que tanto as linhagens de L. edodes como as partes dos basidiomas analisados influenciaram nas respostas nutricionais das amostras. O estípete apresentou as maiores médias de fibra bruta e as menores médias de proteína bruta. O píleo, por sua vez, apresentou as maiores médias de cinzas. Nas avaliações de basidiomas inteiros, as maiores médias, quanto ao teor de proteína bruta, foram obtidas nas linhagens LE-95/07, LE-96/17 e LE-96/18. Já a maior média de fibra bruta foi obtida pela linhagem LE-96/13 de L. edodes. O conteúdo de lipídios do píleo e do estípete não variaram entre as linhagens de L. edodes.Lentinula edodes is high quality food which is rich in proteins, vitamins, and minerals and poor in calories and fat. However, its nutritional value is variable according to the strain grown, processing after harvest, development stage of basidiomata, and substrate used. Thus, the objective of this paper was to carry out the bromatological characterization of basidiomatas from eight (LE-95/01, LE-95/02, LE-95/07, LE-96/17, LE-96/18, LE-98/47, LE-98/55 e LE-96/13 L. edodes strains, whole or in parts (stalk and pileus, produced in E. grandis logs. We verified that both the L. edodes strains and parts of basidiomatas analyzed influenced nutritional responses of the samples. The stalk presented the highest raw fiber averages and the

  4. Consequências da deriva de clomazone e sulfentrazone em clones de E. grandis x E. urophylla Consequences of clomazone and sulfentrazone drift on clones of E. grandis x E. urophylla

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    Ernesto Norio Takahashi

    2009-08-01

    Full Text Available Este trabalho teve por objetivo estudar os efeitos de deriva simulada dos herbicidas clomazone e sulfentrazone em dois clones comerciais de E. grandis x E. urophylla, da Votorantim Celulose e Papel (VCP1 e VCP2. Na simulação, as doses do herbicida clomazone variaram de 0 a 2.000 mL ha-1 e no sulfentrazone, de 0 a 1.500 mL ha-1. As mudas dos dois clones, previamente selecionadas, foram plantadas em vasos com capacidade para 5,0 L. O solo utilizado foi o Neossolo Quartzarênico, sendo a aplicação dos herbicidas realizada 80 dias após o plantio. O delineamento experimental utilizado em cada herbicida foi o de blocos casualizados, com os tratamentos dispostos em esquema fatorial 2 x 8, com três repetições. O efeito do clomazone resultou em folhas novas rosadas, amareladas e, em alguns casos, esbranquiçadas, como um todo ou em parte dela, enquanto as nervuras se mantiveram verdes. Observou-se, também, que as folhas velhas se tornaram mais verdes e grossas. Ocorreu redução nas características de crescimento, variando de 13 a 57%, e as doses consideradas críticas do herbicida clomazone foram de 800 e 1.200 ml ha-1 nos clones VCP1 e VCP2, respectivamente. Com o sulfentrazone, os sintomas da deriva foram necroses generalizadas nas folhas novas e velhas; ao redor da necrose, formaram-se região arroxeada e deformação intensa nas folhas novas e regular nas folhas velhas, bem como houve perda de dominância apical. As características de crescimento indicaram redução de 9 a 66%. A dose crítica desse herbicida foi de 75 ml ha-1 no clone VCP1 e 1.200 ml ha-1 no VCP2. Conclui-se que uma possível deriva dos herbicidas estudados e utilizados em cana-de-açúcar poderá causar prejuízos ao crescimento dos clones avaliados.This research aimed to develop accurate information about the effects of two simulated herbicide drift (clomazone and sulfentrazone on the growth of Votorantim Celulose e Papel Eucalyptus grandis x E. urohylla comercial

  5. Viabilidade Econômica de Sistemas Silvipastoris com Schizolobium parahyba var. amazonicum e Tectona grandis no Pará Economical Viability of Silvopastoral Systems with Schizolobium parahyba var. amazonicum Barneby and Tectona grandis in Para, Brazil

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    Rosana Quaresma Maneschy

    2010-02-01

    Full Text Available

    Os sistemas silvipastoris (SSPs consistem de um conjunto de metodologias que podem ser consideradas promissoras para recuperar áreas de pastagens degradadas da Amazônia, por integrar o cultivo arbóreo ao pecuária tradicional. Embora já utilizados em pequena escala por produtores inovadores, os SSPs carecem de uma base científica que forneça subsídios técnicos ao seu uso mais amplo, sobretudo quando se trata de justificar a viabilidade econômica desses sistemas. O objetivo desse estudo foi analisar a viabilidade econômica de SSPs utilizados por produtores no nordeste do Estado do Pará, comparando-os ao monocultivo tradicional. Os sistemas são compostos basicamente pelo paricá (Schizolobium parahyba var. amazonicum (Huber ex Ducke Barneby ou teca (Tectona grandis L.F., em pastagens de quicuio-da-amazônia (Brachiaria humidicola (Rendle Schweick pastejados por bovinos. A análise de viabilidade econômica foi realizada por meio dos métodos: valor presente líquido (VPL, taxa interna de retorno (TIR e relação benefício/custo (Rb/c, considerando quatro SSPs e o monocultivo de paricá e teca. Os sistemas compostos por teca apresentam maior atratividade econômica do que os com paricá, e dentre eles, os arranjos silvipastoris, em que o gado era próprio, foram considerados de melhor viabilidade que os sistemas em monocultivo ou em que a incorporação animal se dava através do aluguel da pastagem.

     

    doi: 10.4336/2009.pfb.60.49

    The silvopastoral systems (SPSs have been considered promising to integrate tree crops in livestock production systems and reclain large areas of degraded pastures of the Brazilian Amazon. They are used on small scale by innovative farmers, the SPSs lack a scientific basis to provide technical subsidies to its wider use, especially when it comes to justifying the economic viability of these systems. The objective of this study was to analyze the economical viability of SPSs, established in

  6. Durabilidade natural de painéis aglomerados confeccionados com Eucalyptus grandis e Bambusa vulgaris em ensaio de apodrecimento acelerado Natural durability of Eucalyptus grandis and Bambusa vulgaris particleboards under accelerated fungi decay test

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    Diego Martins Stangerlin

    2011-08-01

    Full Text Available O conhecimento acerca da resistência dos compostos ligno-celulósicos ao ataque de microrganismos é primordial para servir de base à prevenção da deterioração e a correta destinação de emprego do material. Nesse sentido, avaliou-se a resistência natural a fungos apodrecedores de painéis aglomerados confeccionados com partículas de madeira (Eucalyptus grandis e/ou bambu (Bambusa vulgaris. Foram produzidas, em laboratório, chapas aglomeradas nas dimensões 50x50x0,95cm e massa específica pré-estabelecida em 0,70g cm-3, nas proporções de 100:0, 75:25, 50:50, 25:75 e 0:100 de madeira e bambu, respectivamente. A massa de partículas representou 91% da massa seca de cada painel, sendo, o restante, formado pelo adesivo ureia-formaldeído (8% e parafina (1%. Para determinação da resistência natural ao ataque de fungos xilófagos, os painéis foram testados em laboratório e utilizados os fungos Gloeophyllum trabeum (podridão parda e Trametes versicolor (podridão branca, de acordo com a ASTM D 2017 (2005. Quanto aos resultados, foi observado que os painéis confeccionados com mistura de partículas de madeira e bambu apresentaram menor resistência ao ataque dos fungos apodrecedores. Painéis que utilizaram apenas bambu ou madeira apresentaram resistência biológica semelhante. Dentre os fungos, T. versicolor atacou mais severamente os painéis.The knowledge about lignocellulosic compounds strength to the attack of microorganisms is essential for preventing deterioration and also for knowing the correct usage of the material. Accordingly it was evaluated the natural resistance of wood panels made of particles of wood (Eucalyptus grandis and/or bamboo (Bambusa vulgaris to decay fungi. Particleboards were produced in laboratory, each of them with dimensions 50x50x0.95cm and density pre-set at 0.70g cm-3, in proportions of 100:0, 75:25, 50:50, 25:75 and 0:100 of wood and bamboo, respectively. The particles mass represented 91% of the

  7. A new species of Crinibracon Quicke (Hymenoptera: Braconidae) parasitic on pupae of Hasora chromus (Cramer) (Lepidoptera: Hesperiidae) from India.

    Science.gov (United States)

    Gupta, Ankita; Achterberg, Cornelis Van; Chitrala, Malathi

    2016-08-29

    A new species, Crinibracon chromusae Gupta & van Achterberg sp. n., parasitic on pupae of Hasora chromus (Cramer) (Hesperiidae) on Millettia (= Pongamia) pinnata (L.) Panigrahi (Fabaceae), is described from India and compared with C. sinicus (Yang, Chen & Liu, 2008) from China, the only other species known with a similar general appearance. For the first time biological information for the genus Crinibracon Quicke, 1988, is given. Three species of hyperparasitoids, Philolema braconidis (Ferrière) (Hymenoptera: Eurytomidae), Nesolynx javanica Ferrière (Hymenoptera: Eulophidae), and an Eupelmus sp. (Hymenoptera: Eupelmidae) emerged along with C. chromusae sp. n. from pupae of H. chromus. The generic placement of this new species along with interesting parasitoid biology is discussed.

  8. Primer registro de Tremex fuscicornis (Hymenoptera: Siricidae para la Argentina en una plantación de álamos en Buenos Aires First record of Tremex fuscicornis (Hymenoptera: Siricidae in Argentina infesting a poplar plantation in Buenos Aires

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    Lucas Landi

    2011-12-01

    Full Text Available La presencia de la "avispa taladradora de las latifoliadas", Tremex fuscicornis Fabricius (Hymenoptera: Siricidae, fue detectada en una plantación de álamos de la provincia de Buenos Aires, lo que constituye el primer registro de la especie para la Argentina. Se describen aspectos morfológicos y biológicos de la misma.The presence of the woodwasp Tremex fuscicornis Fabricius (Hymenoptera: Siricidae was detected in a poplar plantation in Buenos Aires. This is the first record of the species in Argentina. Morphological and biological aspects of the woodwasp are described.

  9. Effect of Different Lignocellulosic Diets on Bacterial Microbiota and Hydrolytic Enzyme Activities in the Gut of the Cotton Boll Weevil (Anthonomus grandis).

    Science.gov (United States)

    Ben Guerrero, Emiliano; Soria, Marcelo; Salvador, Ricardo; Ceja-Navarro, Javier A; Campos, Eleonora; Brodie, Eoin L; Talia, Paola

    2016-01-01

    Cotton boll weevils, Anthonomus grandis , are omnivorous coleopteran that can feed on diets with different compositions, including recalcitrant lignocellulosic materials. We characterized the changes in the prokaryotic community structure and the hydrolytic activities of A. grandis larvae fed on different lignocellulosic diets. A. grandis larvae were fed on three different artificial diets: cottonseed meal (CM), Napier grass (NG) and corn stover (CS). Total DNA was extracted from the gut samples for amplification and sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Proteobacteria and Firmicutes dominated the gut microbiota followed by Actinobacteria, Spirochaetes and a small number of unclassified phyla in CM and NG microbiomes. In the CS feeding group, members of Spirochaetes were the most prevalent, followed by Proteobacteria and Firmicutes. Bray-Curtis distances showed that the samples from the CS community were clearly separated from those samples of the CM and NG diets. Gut extracts from all three diets exhibited endoglucanase, xylanase, β-glucosidase and pectinase activities. These activities were significantly affected by pH and temperature across different diets. We observed that the larvae reared on a CM showed significantly higher activities than larvae reared on NG and CS. We demonstrated that the intestinal bacterial community structure varies depending on diet composition. Diets with more variable and complex compositions, such as CS, showed higher bacterial diversity and richness than the two other diets. In spite of the detected changes in composition and diversity, we identified a core microbiome shared between the three different lignocellulosic diets. These results suggest that feeding with diets of different lignocellulosic composition could be a viable strategy to discover variants of hemicellulose and cellulose breakdown systems.

  10. Evaluación de la toxicidad de proteínas de Bacillus thuringiensis Berliner sobre el picudo del algodonero Anthonomus grandis Boheman

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    Gómez Sylvia

    2006-12-01

    Full Text Available

    El picudo del algodonero, Anthonomus grandis Boheman (Coleoptera: Curculionidae, es considerado la principal plaga del cultivo de algodón en Colombia, no sólo por los daños económicos y sociales que causa, sino porque con sus apariciones tempranas altera e interrumpe el desarrollo de los programas de manejo integrado de plagas. La importancia de un manejo inteligente radica en el hecho de que este insecto se  comporta como plaga ‘clave’, con una capacidad de daño en el cultivo entre 50% y 90%, si no se controla. En el presente estudio se estableció una metodología de bioensayo que determinó la actividad tóxica de  proteínas Cry3Aa y Cry1Ia de cepas de B. thuringiensis sobre larvas de primer instar de A. grandis. En los bioensayos se empleó una dieta artificial mezclada con los extractos bacterianos que contenían el complejo espora-cristal de cada cepa. Los resultados indicaron que la cepa de referencia variedad san diego presentó toxicidad sobre larvas de primer instar de A. grandis, en condiciones de laboratorio, con una concentración letal 50 (CL50 de 147,6143 μg de proteína total por mililitro de volumen final de dieta artificial, representando una alternativa potencial para el manejo de este insecto plaga del cultivo de algodón.

  11. Genome-Wide Analysis of the AP2/ERF Family in Eucalyptus grandis: An Intriguing Over-Representation of Stress-Responsive DREB1/CBF Genes

    Science.gov (United States)

    SanClemente, H.; Mounet, F.; Dunand, C.; Marque, G.; Marque, C.; Teulières, C.

    2015-01-01

    Background The AP2/ERF family includes a large number of developmentally and physiologically important transcription factors sharing an AP2 DNA-binding domain. Among them DREB1/CBF and DREB2 factors are known as master regulators respectively of cold and heat/osmotic stress responses. Experimental Approaches The manual annotation of AP2/ERF family from Eucalyptus grandis, Malus, Populus and Vitis genomes allowed a complete phylogenetic study for comparing the structure of this family in woody species and the model Arabidopsis thaliana. Expression profiles of the whole groups of EgrDREB1 and EgrDREB2 were investigated through RNAseq database survey and RT-qPCR analyses. Results The structure and the size of the AP2/ERF family show a global conservation for the plant species under comparison. In addition to an expansion of the ERF subfamily, the tree genomes mainly differ with respect to the group representation within the subfamilies. With regard to the E. grandis DREB subfamily, an obvious feature is the presence of 17 DREB1/CBF genes, the maximum reported to date for dicotyledons. In contrast, only six DREB2 have been identified, which is similar to the other plants species under study, except for Malus. All the DREB1/CBF and DREB2 genes from E. grandis are expressed in at least one condition and all are heat-responsive. Regulation by cold and drought depends on the genes but is not specific of one group; DREB1/CBF group is more cold-inducible than DREB2 which is mainly drought responsive. Conclusion These features suggest that the dramatic expansion of the DREB1/CBF group might be related to the adaptation of this evergreen tree to climate changes when it expanded in Australia. PMID:25849589

  12. Genome-wide analysis of the AP2/ERF family in Eucalyptus grandis: an intriguing over-representation of stress-responsive DREB1/CBF genes.

    Directory of Open Access Journals (Sweden)

    P B Cao

    Full Text Available The AP2/ERF family includes a large number of developmentally and physiologically important transcription factors sharing an AP2 DNA-binding domain. Among them DREB1/CBF and DREB2 factors are known as master regulators respectively of cold and heat/osmotic stress responses.The manual annotation of AP2/ERF family from Eucalyptus grandis, Malus, Populus and Vitis genomes allowed a complete phylogenetic study for comparing the structure of this family in woody species and the model Arabidopsis thaliana. Expression profiles of the whole groups of EgrDREB1 and EgrDREB2 were investigated through RNAseq database survey and RT-qPCR analyses.The structure and the size of the AP2/ERF family show a global conservation for the plant species under comparison. In addition to an expansion of the ERF subfamily, the tree genomes mainly differ with respect to the group representation within the subfamilies. With regard to the E. grandis DREB subfamily, an obvious feature is the presence of 17 DREB1/CBF genes, the maximum reported to date for dicotyledons. In contrast, only six DREB2 have been identified, which is similar to the other plants species under study, except for Malus. All the DREB1/CBF and DREB2 genes from E. grandis are expressed in at least one condition and all are heat-responsive. Regulation by cold and drought depends on the genes but is not specific of one group; DREB1/CBF group is more cold-inducible than DREB2 which is mainly drought responsive.These features suggest that the dramatic expansion of the DREB1/CBF group might be related to the adaptation of this evergreen tree to climate changes when it expanded in Australia.

  13. Proteômica quantitativa e metabolômica do híbrido Eucalyptus grandis x E. camaldulensis, tolerante e susceptível ao déficit hídrico

    OpenAIRE

    Janaina de Santana Borges

    2016-01-01

    O E. grandis x E. camaldulensis possui características favoráveis de adaptação à seca, conferidas pelo E. camaldulensis e qualidade da madeira para papel e celulose, conferida pelo E. grandis. Esta adaptação à seca está relacionada a fatores fisiológicos e também moleculares, expressos em sua proteoma e metaboloma, que se alteram na presença do estresse. Objetiva-se neste trabalho estudar as respostas fisiológicas, proteômicas e metabolômicas (metabólitos primários) diferencialmente expressos...

  14. Primer registro para el Perú de Nematophila grandis (Diesing, 1839 Travassos, 1934 (Trematoda, Diplodiscidae en Podocnemis unifilis (Troschel, 1848 (Testudines, Pelomedusidae

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    Patricia Salízar

    2013-06-01

    Full Text Available El presente trabajo registra por primera vez para el Perú a Nematophila grandis (Diesing, 1839 Travassos, 1934, en la tortuga de río Podocnemis unifilis «taricaya». Los hospederos fueron colectados en las localidades del Río Putumayo, Samiria, Iquitos (Loreto y Manu (Madre de Dios. El material identificado pertenece a la Colección Helmintológica del Departamento de Protozoología, Helmintología e Invertebrados Afines del Museo de Historia Natural de la Universidad Nacional Mayor de San Marcos.

  15. Microbial biomass and activity in litter during the initial development of pure and mixed plantations of Eucalyptus grandis and Acacia mangium

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    Daniel Bini

    2013-02-01

    Full Text Available Studies on microbial activity and biomass in forestry plantations often overlook the role of litter, typically focusing instead on soil nutrient contents to explain plant and microorganism development. However, since the litter is a significant source of recycled nutrients that affect nutrient dynamics in the soil, litter composition may be more strongly correlated with forest growth and development than soil nutrient contents. This study aimed to test this hypothesis by examining correlations between soil C, N, and P; litter C, N, P, lignin content, and polyphenol content; and microbial biomass and activity in pure and mixed second-rotation plantations of Eucalyptus grandis and Acacia mangium before and after senescent leaf drop. The numbers of cultivable fungi and bacteria were also estimated. All properties were correlated with litter C, N, P, lignin and polyphenols, and with soil C and N. We found higher microbial activity (CO2 evolution in litter than in soil. In the E. grandis monoculture before senescent leaf drop, microbial biomass C was 46 % higher in litter than in soil. After leaf drop, this difference decreased to 16 %. In A. mangium plantations, however, microbial biomass C was lower in litter than in soil both before and after leaf drop. Microbial biomass N of litter was approximately 94 % greater than that of the soil in summer and winter in all plantations. The number of cultivable fungi and bacteria increased after leaf drop, especially so in the litter. Fungi were also more abundant in the E. grandis litter. In general, the A. mangium monoculture was associated with higher levels of litter lignin and N, especially after leaf drop. In contrast, the polyphenol and C levels in E. grandis monoculture litter were higher after leaf drop. These properties were negatively correlated with total soil C and N. Litter in the mixed stands had lower C:N and C:P ratios and higher N, P, and C levels in the microbial biomass. This suggests more

  16. First occurrence of Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) preying on defoliating caterpillars of oil palm in the state of Para, Brazil

    International Nuclear Information System (INIS)

    Ribeiro, Rafael C.; Lemos, Walkymario P.; Muller, Antonio A.

    2010-01-01

    The oil palm Elaeis guineensis is usually attacked by pests, particularly, defoliating caterpillars. Between 2004 and 2006 a stinkbug predator (Asopinae) was registered preying on caterpillars of Brassolis sophorae L., Opsiphanes invirae Hubner (Lepidoptera: Nymphalidae) and Sibine spp. (Lepidoptera: Limacodidae), reducing their populations in commercial oil palm plantations in the State of Para, Brazil. Specimens of the natural enemy were collected, mounted, and identified as Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae), corresponding to the first report of the occurrence of this stinkbug attacking defoliating caterpillars of oil palm in Brazil. (author)

  17. Desarrollo de funciones de índice de sitio para Eucalyptus grandis cultivado en la Mesopotamia argentina

    Directory of Open Access Journals (Sweden)

    CRECHI, E.H

    2011-12-01

    Full Text Available ResumenSe desarrollaron modelos para la estimación del índice de sitio para Eucalyptus grandis implantado en la Mesopotamia argentina. Dicha estimación se llevó a cabo a través de la evaluación de una base general de datos que contó con 106 parcelas (439 mediciones, de las cuales 48 (197 mediciones pertenecían a la zona Misiones-NE Corrientes y 58 (242 mediciones a la zona Concordia-Monte Caseros (NE Entre Ríos-SE Corrientes. Analizadas las curvas promedio de evolución de la altura dominante con la edad de las dos regiones agroecológicas consideradas, se observó que sus pendientes diferían por lo que se ajustaron funciones de sitio para cada una de ellas. Fueron empleados tres métodos tradicionales, curva guía, reparametrización y ecuaciones diferenciales. El procedimiento elegido dependió de la calidad de información disponible en las zonas agroecológicas bajo estudio, aunque se priorizó que cumplieran con algunos criterios deseables para las funciones de sitio como el polimorfismo y que las alturas sean invariantes respecto de la edad índice, entre otros criterios, como también la de estabilidad frente a sus propios datos. Para la zona agro-ecológica Misiones-NE Corrientes se lograron buenos ajustes con el método de la curva Guía, mientras que para la zona Concordia-Monte Caseros se optó por el modelo de Schumacher ajustado mediante el método de aproximación algebraica diferencial (ADA.AbstractEquations for the prediction of site index of Eucalyptus grandis planted in the Mesopotamia region of Argentina, were developed. With this purpose a data base with 106 plots (439 pairs age-hdom was used; of it 48 (197 pairs age-Hdom belongs to Misiones-NE Corrientes agro ecological zone and the other 58 plots (242 pairs age-hdom belongs to the zone Concordia-Monte Caseros (SE Corrientes – NE Entre Ríos. Analyzed the evolution of the relationship between age-hdom of both zones it was determined that the slopes of the models

  18. Diversidade de abelhas (Hymenoptera, Apidae) ao longo de um gradiente latitudinal na Mata Atlântica

    OpenAIRE

    Gonçalves,Rodrigo Barbosa; Brandão,Carlos Roberto Ferreira

    2008-01-01

    A Mata Atlântica é um dos ambientes mais ricos e ameaçados do mundo, o que deveria ter estimulado em muito o estudo e a conservação do Bioma, mas a fauna de Hymenoptera permanece ainda relativamente pouco conhecida. Em especial, a fauna de abelhas da floresta ombrófila densa é pouco estudada em comparação à fauna das áreas abertas brasileiras. O projeto temático "Biodiversidade de Hymenoptera e Isoptera: riqueza e diversidade ao longo de um gradiente latitudinal na Mata Atlântica - a floresta...

  19. Relative contributions of copper oxide nanoparticles and dissolved copper to Cu uptake kinetics of Gulf killifish (Fundulus grandis) embryos

    Science.gov (United States)

    Jiang, Chuanjia; Castellon, Benjamin T.; Matson, Cole W.; Aiken, George R.; Hsu-Kim, Heileen

    2017-01-01

    The toxicity of soluble metal-based nanomaterials may be due to the uptake of metals in both dissolved and nanoparticulate forms, but the relative contributions of these different forms to overall metal uptake rates under environmental conditions are not quantitatively defined. Here, we investigated the linkage between the dissolution rates of copper(II) oxide (CuO) nanoparticles (NPs) and their bioavailability to Gulf killifish (Fundulus grandis) embryos, with the aim of quantitatively delineating the relative contributions of nanoparticulate and dissolved species for Cu uptake. Gulf killifish embryos were exposed to dissolved Cu and CuO NP mixtures comprising a range of pH values (6.3–7.5) and three types of natural organic matter (NOM) isolates at various concentrations (0.1–10 mg-C L–1), resulting in a wide range of CuO NP dissolution rates that subsequently influenced Cu uptake. First-order dissolution rate constants of CuO NPs increased with increasing NOM concentration and for NOM isolates with higher aromaticity, as indicated by specific ultraviolet absorbance (SUVA), while Cu uptake rate constants of both dissolved Cu and CuO NP decreased with NOM concentration and aromaticity. As a result, the relative contribution of dissolved Cu and nanoparticulate CuO species for the overall Cu uptake rate was insensitive to NOM type or concentration but largely determined by the percentage of CuO that dissolved. These findings highlight SUVA and aromaticity as key NOM properties affecting the dissolution kinetics and bioavailability of soluble metal-based nanomaterials in organic-rich waters. These properties could be used in the incorporation of dissolution kinetics into predictive models for environmental risks of nanomaterials.

  20. Acúmulo e distribuição de nutrientes em Eucalyptus grandis sob diferentes densidades populacionais

    Directory of Open Access Journals (Sweden)

    F. P. Leite

    1998-09-01

    Full Text Available Em experimento realizado na região de Santa Bárbara (MG, avaliou-se o efeito da densidade populacional de plantas (DP sobre as relações nutricionais de Eucalyptus grandis. Os tratamentos consistiram de sete DP, variando de 500 a 5.000 plantas por ha-1. As avaliações, realizadas em julho de 1994, quando as plantas tinham 31 meses de idade, consistiram da coleta e análise química de amostras de tecidos vegetais dos componentes da parte aérea de uma árvore média de cada parcela e da serrapilheira. Determinaram-se as concentrações e calcularam-se os conteúdos e a eficiência de utilização de N, P, K, Ca, e Mg. As relações entre os conteúdos de P, N, K e Mg na biomassa da parte aérea, os coeficiente de utilização (CUB de P, Ca e Mg pelo tronco e a DP foram mais bem descritas por equações quadráticas. O conteúdo de Ca relacionou-se de modo linear, e os CUB de K e N não foram alterados com a DP. Analisando o balanço de nutrientes, observou-se que as quantidades de P disponível, K e Ca trocáveis existentes no solo (camada de 0 a 195 cm não seriam suficientes para suprir a demanda nutricional das plantas em um novo ciclo de crescimento, até a idade de 31 meses, em DP superiores a 833 árvores por ha-1.

  1. Ethanol extracts of Cassia grandis and Tabernaemontana cymosa inhibit the in vitro replication of dengue virus serotype 2

    Directory of Open Access Journals (Sweden)

    Carolina Hernández-Castro

    2015-02-01

    Full Text Available Objective: To determine the antiviral activity of ethanol extracts derived from Cassia grandis leaves and Tabernaemontana cymosa bark against two dengue virus (DENV serotype 2 strains DENV-2/NG and DENV-2/1 6681 in two cell lines susceptible to infection, VERO and U937. Methods: The cytotoxic concentration 50 (CC50 was assessed using the MTT method, and the effective concentration 50 (EC50 was determined using the technique of inhibiting the production of infectious viral particles by the plating method. Further testing of dose-response inhibition was performed, and three experimental approaches were evaluated (pre-, trans- and posttreatment to determine the effect of the extracts according to the time of administration. Finally, a preliminary phytochemical analysis for both extracts was performed. Results: The cytotoxicity of the extracts was low (CC50>300 µg/mL, and the U937 cell line was more sensitive to the antiproliferative effect of both extracts. When the virus strain-dependent selectivities of the extracts were compared, it was found that both extracts were more selective in cultures infected with the DENV-2/NG strain than in those infected with the DENV-2/16681 strain. A dose-dependent inhibitory effect of the extracts was not observed in any of the evaluations. Finally, the highest inhibition was detected with the post-treatment approach with the Tabernaemontana cymosa extract (99.9% in both cell lines. Conclusions: A therapy with compounds derived from these extracts would inhibit viral replication and affect steps after viral internalization.

  2. Influence of euthanasia method on blood and gill variables in normoxic and hypoxic Gulf killifish Fundulus grandis.

    Science.gov (United States)

    Larter, K F; Rees, B B

    2017-06-01

    In many experiments, euthanasia, or humane killing, of animals is necessary. Some methods of euthanasia cause death through cessation of respiratory or cardiovascular systems, causing oxygen levels of blood and tissues to drop. For experiments where the goal is to measure the effects of environmental low oxygen (hypoxia), the choice of euthanasia technique, therefore, may confound the results. This study examined the effects of four euthanasia methods commonly used in fish biology (overdose of MS-222, overdose of clove oil, rapid cooling and blunt trauma to the head) on variables known to be altered during hypoxia (haematocrit, plasma cortisol, blood lactate and blood glucose) or reflecting gill damage (trypan blue exclusion) and energetic status (ATP, ADP and ATP:ADP) in Gulf killifish Fundulus grandis after 24 h exposure to well-aerated conditions (normoxia, 7·93 mg O 2  l -1 , c. 150 mm Hg or c. 20 kPa) or reduced oxygen levels (0·86 mg O 2  l -1 , c. 17 mm Hg or c. 2·2 kPa). Regardless of oxygen treatment, fish euthanized by an overdose of MS-222 had higher haematocrit and lower gill ATP:ADP than fish euthanized by other methods. The effects of 24 h hypoxic exposure on these and other variables, however, were equivalent among methods of euthanasia (i.e. there were no significant interactions between euthanasia method and oxygen treatment). The choice of an appropriate euthanasia method, therefore, will depend upon the magnitude of the treatment effects (e.g. hypoxia) relative to potential artefacts caused by euthanasia on the variables of interest. © 2017 The Fisheries Society of the British Isles.

  3. Expression patterns of flowering genes in leaves of 'Pineapple' sweet orange [Citrus sinensis (L.) Osbeck] and pummelo (Citrus grandis Osbeck).

    Science.gov (United States)

    Pajon, Melanie; Febres, Vicente J; Moore, Gloria A

    2017-08-30

    In citrus the transition from juvenility to mature phase is marked by the capability of a tree to flower and fruit consistently. The long period of juvenility in citrus severely impedes the use of genetic based strategies to improve fruit quality, disease resistance, and responses to abiotic environmental factors. One of the genes whose expression signals flower development in many plant species is FLOWERING LOCUS T (FT). In this study, gene expression levels of flowering genes CiFT1, CiFT2 and CiFT3 were determined using reverse-transcription quantitative real-time PCR in citrus trees over a 1 year period in Florida. Distinct genotypes of citrus trees of different ages were used. In mature trees of pummelo (Citrus grandis Osbeck) and 'Pineapple' sweet orange (Citrus sinensis (L.) Osbeck) the expression of all three CiFT genes was coordinated and significantly higher in April, after flowering was over, regardless of whether they were in the greenhouse or in the field. Interestingly, immature 'Pineapple' seedlings showed significantly high levels of CiFT3 expression in April and June, while CiFT1 and CiFT2 were highest in June, and hence their expression induction was not simultaneous as in mature plants. In mature citrus trees the induction of CiFTs expression in leaves occurs at the end of spring and after flowering has taken place suggesting it is not associated with dormancy interruption and further flower bud development but is probably involved with shoot apex differentiation and flower bud determination. CiFTs were also seasonally induced in immature seedlings, indicating that additional factors must be suppressing flowering induction and their expression has other functions.

  4. The chronic toxicity of mineral oil-wet and synthetic liquid-wet cuttings on an estuarine fish, Fundulus grandis

    International Nuclear Information System (INIS)

    Jones, F.V.; Rushing, J.H.; Churan, M.A.

    1991-01-01

    One of the major factors concerning oil-wet cuttings discharges in the long-term effect on the aquatic environment. This paper investigates the survival and growth of the mud minnow, Fundulus grandis, when exposed to different concentrations of mineral oil-wet and synthetic liquid-wet cuttings in flow-through bioassay chambers. The test fluids were a low aromatic mineral oil-based mud (MOBM) and a synthetic liquid-based mud (SBM). Each test fluid was added to a container of dried water-based cuttings which contained minimum hydrocarbons. The fluid and cuttings were mixed to obtain the desired test concentrations. The study tested three concentrations (1%, 5%, and 8.4% by dry weight) of each fluid on the cuttings. Growth rates during the 3-day period were modest with fish in 1% MOBM losing weight. The highest percent growth rates were obtained with fish cultured in 5% SBM and the controls. However, overall growth was not significantly different between treatments. Mean growth did show a significant difference between controls and 5% SBM, and other treatments. The cuttings of the 5% and 8.4% concentrations of both treatments looked like paste. These pastes may have slowed the movement of organics off of the cuttings beds and affected biodegradability and growth rates. Cuttings in the controls and 1% concentrations stayed suspended in the water column longer when disturbed and looked more like loose gravel. Uptake of the MOBM occurred in the internal organs and tissue of fish. No such uptake was observed in the fish tissue with the SBM; a very low level of synthetic liquid was detected in one gut sample only. This may be the major reason for the variations noted in growth rates. Fish mortality during the study was related to the buildup of anaerobic conditions and insufficient aeration. Fish survival for the entire study was 94%

  5. [Rapid Identification of Epicarpium Citri Grandis via Infrared Spectroscopy and Fluorescence Spectrum Imaging Technology Combined with Neural Network].

    Science.gov (United States)

    Pan, Sha-sha; Huang, Fu-rong; Xiao, Chi; Xian, Rui-yi; Ma, Zhi-guo

    2015-10-01

    To explore rapid reliable methods for detection of Epicarpium citri grandis (ECG), the experiment using Fourier Transform Attenuated Total Reflection Infrared Spectroscopy (FTIR/ATR) and Fluorescence Spectrum Imaging Technology combined with Multilayer Perceptron (MLP) Neural Network pattern recognition, for the identification of ECG, and the two methods are compared. Infrared spectra and fluorescence spectral images of 118 samples, 81 ECG and 37 other kinds of ECG, are collected. According to the differences in tspectrum, the spectra data in the 550-1 800 cm(-1) wavenumber range and 400-720 nm wavelength are regarded as the study objects of discriminant analysis. Then principal component analysis (PCA) is applied to reduce the dimension of spectroscopic data of ECG and MLP Neural Network is used in combination to classify them. During the experiment were compared the effects of different methods of data preprocessing on the model: multiplicative scatter correction (MSC), standard normal variable correction (SNV), first-order derivative(FD), second-order derivative(SD) and Savitzky-Golay (SG). The results showed that: after the infrared spectra data via the Savitzky-Golay (SG) pretreatment through the MLP Neural Network with the hidden layer function as sigmoid, we can get the best discrimination of ECG, the correct percent of training set and testing set are both 100%. Using fluorescence spectral imaging technology, corrected by the multiple scattering (MSC) results in the pretreatment is the most ideal. After data preprocessing, the three layers of the MLP Neural Network of the hidden layer function as sigmoid function can get 100% correct percent of training set and 96.7% correct percent of testing set. It was shown that the FTIR/ATR and fluorescent spectral imaging technology combined with MLP Neural Network can be used for the identification study of ECG and has the advantages of rapid, reliable effect.

  6. Photosynthetic pigments and stomatal conductance in ecotypes of copoazu (Theobroma grandi orum Willd. Ex. Spreng K. Schum..

    Directory of Open Access Journals (Sweden)

    Juan Carlos Suárez-Salazar

    2016-12-01

    Full Text Available The objective of this work was to evaluate the variability of photosynthetic pigment content and daily stomatal conductance was evaluated in relation to environmental variables in Copoazú (Theobroma grandi orum ecotypes. The ecotypes used were part of the germoplasm bank of the University of the Amazon (Colombia. The study was carried out during the year 2015. Four leaves of the average stratum of four plants were collected for each ecotype, to extract and read at different levels of absorbance and determine the content of photosynthetic pigments. During the hours of 04:00 a.m. to 6:00 p.m., the stomatal conductance (gs was monitored for environmental variables (relative humidity, air temperature, radiation and vapor pressure de cit (VPD. An analysis of variance was made using the Tukey test, correlations and regressions were made between gs and environmental variables. The contents of chlorophyll a, b, total and carotenoids among ecotypes were different (P<0.0001, the ecotype UA-31 presented the highest values, contrasting with the ecotype UA-37. Concerning gs, the interaction ecotype*hour showed signi cant differences (P<0.0001 .The ecotypes that presented the highest values of gs were UA-67 and UA-039, (P<0.0001, radiation (-0.91, P<0.0001 and DPV (-0.94; P<0.0001 0.0001.The results suggest that ecotypes UA-039 and UA-31 were the most suitable in terms of gaseous exchange and content of photosynthetic pigments.

  7. Propriedades de chapas de flocos fabricadas com adesivo de uréia-formaldeído e de taninos da casca de Eucalyptus grandis W. Hill ex Maiden ou de Eucalyptus pellita F. Muell. Properties of flakeboards made from urea-formaldehyde and bark tannins adhesives of Eucalyptus grandis or Eucalyptus pellita

    Directory of Open Access Journals (Sweden)

    Angélica de Cássia Oliveira Carneiro

    2004-10-01

    Full Text Available Os taninos foram extraídos da casca de Eucalyptus grandis e Eucalyptus pellita, com água quente, à qual se adicionaram 4,5% de sulfito de sódio, durante três horas. As temperaturas da solução foram iguais a 70 e 100 ºC para Eucalyptus grandis e Eucalyptus pellita, respectivamente. Para a produção dos adesivos e com o intuito de reduzir a sua viscosidade, os taninos foram sulfitados com sulfito de sódio e ácido acético. Formulações adesivas foram preparadas adicionando-se 0, 25, 50, 75 ou 100% de adesivos tânicos ao adesivo comercial de uréia-formaldeído. Foram fabricadas chapas de flocos de Pinus elliottii Engelm. e Eucalyptus grandis, utilizando-se 8% da formulação adesiva. As propriedades das chapas foram determinadas segundo a norma ASTM D-1037, de 1993. Observou-se que as propriedades das chapas foram superiores ao mínimo estabelecido pela norma ANSI/A 280.1-93, exceto no caso da resistência à umidade. Verificou-se, ainda, que o emprego de uma formulação adesiva contendo resina à base de uréia-formaldeído e tanino-formaldeído pode melhorar algumas propriedades.Bark tannins of Eucalyptus grandis and Eucalyptus pellita were extracted with 4,5% sodium sulfite in hot water solution for a period of three hours. Solution temperatures were 70 and 100ºC, for Eucalyptus grandis and Eucalyptus pellita bark respectively. Tannins were reacted with acetic acid and sodium sulfite to reduce adhesive viscosity. Adhesive formulations were prepared adding 0, 25, 50, 75 or 100% of tannin adhesives to the commercial urea-formaldehyde adhesive. Flakeboards were fabricated with 8% resin content. Board properties were determined according to ASTM D-1037 standards. Except for humidity, all board properties were superior to the values established by ANSI.A 208.1-93 commercial standard. Addition of tannins to the urea-formaldehyde adhesive improved some properties.

  8. Método de diagnóstico para el monitoreo de resistencia a insecticidas en poblaciones de "picudo del algodonero", Anthonomus grandis (Coleoptera: Curculionidae A diagnostic test for insecticide resistance monitoring in "cotton boll weevil" Anthonomus grandis (Coleoptera: Curculionidae populations

    Directory of Open Access Journals (Sweden)

    Teodoro Stadler

    2009-06-01

    Full Text Available El control de las poblaciones de Anthonomus grandis Boheman, por debajo de su umbral de daño económico durante el ciclo del cultivo del algodón, se realiza en forma efectiva hasta el momento, a través de insecticidas de síntesis. La presión selectiva de las aplicaciones extensivas e intensivas de insecticidas hace imperativa la detección temprana de focos de resistencia a los mismos, en función de un correcto manejo del fenómeno. Se desarrolló un método de diagnóstico de resistencia para A. grandis a partir de la técnica "vial test", que fue adaptada en forma de "kit" para el monitoreo rápido y sencillo de los focos de resistencia en el campo. La toxicidad (CL99, para calcular la concentración discriminante (CD del insecticida y la preparación del "kit", se obtiene a partir de bioensayos de laboratorio con una cepa normal susceptible de A. grandis. Se determinó la vida media de los insecticidas dentro de los viales por CIPAC MT 46, para establecer una fecha de vencimiento del "kit". La CD y el método en su conjunto fueron validados a través de ensayos a campo. El "kit", usado en el monitoreo de resistencia en el campo, fue especialmente diseñado para ser utilizado en las condiciones geográficas, económicas y socio-culturales presentes en la región algodonera argentina. La implementación de esta técnica permitirá conseguir la información necesaria, y así obtener una apropiada alternancia de insecticidas. Como consecuencia, se prevé una reducción de impacto ambiental de las prácticas agronómicas en el control de plagas en algodón.The in-season control of the cotton boll weevil Anthonomus grandis Boheman is done by insecticide application, which so far is the only effective way to reduce boll weevil populations to levels below economic significance. The extensive and intensive control actions with insecticides cause selective pressure on pest populations. Thus, to achieve an accurate insecticide resistance

  9. Effects of insecticides and defoliants applied alone and in combination for control of overwintering boll weevil (Anthonomus grandis; Coleoptera: Curculionidae)--laboratory and field studies.

    Science.gov (United States)

    Greenberg, Shoil M; Sappington, Thomas W; Elzen, Gary W; Norman, John W; Sparks, Alton N

    2004-09-01

    In laboratory, greenhouse and field tests, we determined the effects of combining full rates of the defoliants tribufos and thidiazuron and the herbicide thifensulfuron-methyl with half rates of the insecticides lambda-cyhalothrin or azinphos-methyl, and the combination of tribufos and thidiazuron, both in half rates, on mortality of the boll weevil, Anthonomus grandis grandis Boheman and on the quality of defoliation. Tribufos, 0.47 kg ha(-1) and tribufos, 0.235 kg ha(-1) + thidiazuron, 0.125 kg ha(-1) exhibited a slightly toxic effect to boll weevil, while tribufos, 0.47 kg ha(-1) + lambda-cyhalothrin, 0.019 kg ha(-1), tribufos, 0.47 kg ha(-1) + azinphos-methyl, 0.14 kg ha(-1), and tribufos, 0.235 kg ha(-1) + thidiazuron, 0.125 kg ha(-1) + azinphos-methyl, 0.14 kg ha(-l), provided control of boll weevil as good as or better than full-rate azinphos-methyl or lambda-cyhalothrin alone owing to synergistic effects. Thidiazuron or thifensulfuron-methyl alone or in combination with insecticides did not affect boll weevil mortality. Treatment with tribufos + thidiazuron, both at half rate, significantly increased defoliation compared to full rates of tribufos or thidiazuron alone, and provided adequate defoliation for approximately the same cost per hectare.

  10. A diverse family of serine proteinase genes expressed in cotton boll weevil (Anthonomus grandis): implications for the design of pest-resistant transgenic cotton plants.

    Science.gov (United States)

    Oliveira-Neto, Osmundo B; Batista, João A N; Rigden, Daniel J; Fragoso, Rodrigo R; Silva, Rodrigo O; Gomes, Eliane A; Franco, Octávio L; Dias, Simoni C; Cordeiro, Célia M T; Monnerat, Rose G; Grossi-De-Sá, Maria F

    2004-09-01

    Fourteen different cDNA fragments encoding serine proteinases were isolated by reverse transcription-PCR from cotton boll weevil (Anthonomus grandis) larvae. A large diversity between the sequences was observed, with a mean pairwise identity of 22% in the amino acid sequence. The cDNAs encompassed 11 trypsin-like sequences classifiable into three families and three chymotrypsin-like sequences belonging to a single family. Using a combination of 5' and 3' RACE, the full-length sequence was obtained for five of the cDNAs, named Agser2, Agser5, Agser6, Agser10 and Agser21. The encoded proteins included amino acid sequence motifs of serine proteinase active sites, conserved cysteine residues, and both zymogen activation and signal peptides. Southern blotting analysis suggested that one or two copies of these serine proteinase genes exist in the A. grandis genome. Northern blotting analysis of Agser2 and Agser5 showed that for both genes, expression is induced upon feeding and is concentrated in the gut of larvae and adult insects. Reverse northern analysis of the 14 cDNA fragments showed that only two trypsin-like and two chymotrypsin-like were expressed at detectable levels. Under the effect of the serine proteinase inhibitors soybean Kunitz trypsin inhibitor and black-eyed pea trypsin/chymotrypsin inhibitor, expression of one of the trypsin-like sequences was upregulated while expression of the two chymotrypsin-like sequences was downregulated. Copyright 2004 Elsevier Ltd.

  11. Crecimiento y propiedades fisico-mecanicas de la madera de teca (Tectona grandis de 17 anos de edad en San Joaquin de Abangares, Costa Rica

    Directory of Open Access Journals (Sweden)

    Fernando Castro

    2000-01-01

    Full Text Available Growth and physical-mechanical properties of 17 years old teak (Tectona grandis growing in San Joaquin, Abangares, Costa Rica. The national and international market of forest products, from certifieddad. managment forests and plantations, is increasingly more demanding as to the standards and characteristics of high quality. The physicalmecanicas mechanical properties of the teak (Tectona grandis, growing in San Joaqufn de Abangares, Costa Rica, at 30 m and 100 16´ north latitude, are determined according to the ASTM standard Destudiadas 143-83. The physical properties of teak timber studied were: basic specific weight, radial, tangential and volumetric contractions, contraction ratio, dry and green density and point of fiber saturation. The mechanical properties studied were: static flexure, shear, hardness, parallel and perpendicular compression. Also included were comparisons with teak timber harvested in other places and latitudes, as well as other hardwood species. The coefficient of variation of the basic specific weight of the San Joaquin de Abangares teak (3.4% is half that of the Quepos teak (7% and almost one third of the average of 50 species (10%, which is an...

  12. Effect of Thermo-Treatment on the Physical and Mechanical, Color, Fungal Durability of Wood of Tectona Grandis and Gmelina Arborea from Forest Plantations

    Directory of Open Access Journals (Sweden)

    Luis Diego MÉNDEZ-MEJÍAS

    2018-02-01

    Full Text Available This study evaluated the effect of thermo-treatment (THT at 4 temperatures on the density, shrinking, mass loss, moisture absorption, color, durability in terms of resistance to decay, flexural strength, tensile adhesion of glue line and the infrared spectrum of the wood of Tectona grandis and Gmelina arborea. Sapwood, heartwood and radial and tangential grain patterns were studied. The results showed that the THT temperature decreases the density, the percentage of moisture absorption, the modulus of elasticity and modulus of rupture in the flexure test and the tensile adhesion of glue line. The percentage of shrinking and durability presented irregular behavior relative to the THT temperature. The percentage of mass loss increased with increasing THT temperature in both species. The total color change (∆E* of thermo-treated wood (THTwood also increased with increasing THT temperature. Sapwood of T. grandis and G. arborea, having clearer shades, showed a more noticeable color change compared to hardwood; however, no significant differences were obtained between some of the THT temperatures.DOI: http://dx.doi.org/10.5755/j01.ms.24.1.17545

  13. Leaf cDNA-AFLP analysis reveals novel mechanisms for boron-induced alleviation of aluminum-toxicity in Citrus grandis seedlings.

    Science.gov (United States)

    Wang, Liu-Qing; Yang, Lin-Tong; Guo, Peng; Zhou, Xin-Xing; Ye, Xin; Chen, En-Jun; Chen, Li-Song

    2015-10-01

    Little information is available on the molecular mechanisms of boron (B)-induced alleviation of aluminum (Al)-toxicity. 'Sour pummelo' (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing different concentrations of B (2.5 or 20μM H3BO3) and Al (0 or 1.2mM AlCl3·6H2O). B alleviated Al-induced inhibition in plant growth accompanied by lower leaf Al. We used cDNA-AFLP to isolate 127 differentially expressed genes from leaves subjected to B and Al interactions. These genes were related to signal transduction, transport, cell wall modification, carbohydrate and energy metabolism, nucleic acid metabolism, amino acid and protein metabolism, lipid metabolism and stress responses. The ameliorative mechanisms of B on Al-toxicity might be related to: (a) triggering multiple signal transduction pathways; (b) improving the expression levels of genes related to transport; (c) activating genes involved in energy production; and (d) increasing amino acid accumulation and protein degradation. Also, genes involved in nucleic acid metabolism, cell wall modification and stress responses might play a role in B-induced alleviation of Al-toxicity. To conclude, our findings reveal some novel mechanisms on B-induced alleviation of Al-toxicity at the transcriptional level in C. grandis leaves. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. The first record of the genus Tanaostigma (Hymenoptera: Tanaostigmatidae) in the Old World, with the description of a new species from India.

    Science.gov (United States)

    Gupta, Ankita; Joshi, Sunil

    2016-11-15

    Tanaostigma Howard (Hymenoptera: Tanaostigmatidae) is recorded for the first time in the fauna of the Old World, with T. indica Gupta sp. n.  described and illustrated from southern India, reared from Millettia (=Pongamia) pinnata (Fabaceae).

  15. Effects of feeding frequency and sugar concentration on behavior and longevity of the adult aphid parasitoid: Aphidius ervi (Haliday) (Hymenoptera: Braconidae)

    NARCIS (Netherlands)

    Azzouz, H.; Giordanengo, P.; Wäckers, F.L.; Kaiser, L.

    2004-01-01

    Aphidius ervi (Haliday) (Hymenoptera: Braconidae) is a solitary aphid endoparasitoid. Adults feed on honeydew and possibly on other sugar sources such as nectar. Sugar sources can vary qualitatively and quantitatively according to biotic factors and environmental conditions. Experiments were

  16. Oviposition behavior and survival of Tamarixia radiata (Hymenoptera: Eulophidae), an ectoparasitoid of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), on hosts exposed to an entomopathogenic fungus

    Science.gov (United States)

    Antagonistic interactions between the nymphal parasitoid, Tamarixia radiata Waterston (Hymenoptera: Eulophidae), and the ARSEF 3581 isolate of the entomopathogenic fungus, Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae) could disrupt biological control of the Asian citrus psyllid, Diaphorina ...

  17. Notes on Apidae and Vespidae (Hymenoptera) Species Collected by Bait Traps in OrganicVineyard and Orchards of Kemalpaşa (İzmir), Western Turkey

    OpenAIRE

    ÜZÜM, Ahu; TANYERİ, Rukiye; GÜLPERÇİN, Nilay; TEZCAN, Serdar; YILDIRIM, Erol

    2010-01-01

    Hymenoptera species collected by bait traps during the months of June-October in organic vineyard and orchards in Kemalpaşa district, (İzmir) of Western Turkey were evaluated in this study. As a result, six species belonging 2007 to two families of Hymenoptera were determined. Those were Apis mellifera Linnaeus, 1758, Vespula germanica (Fabricius, 1793), Vespa crabro Linnaeus, 1758, Vespa orientalis Linnaeus, 1771, Polistes dominulus (Christ, 1791) and Polistes gallicus (Linnaeus, 1767). Amon...

  18. Improved sensitivity to venom specific-immunoglobulin E by spiking with the allergen component in Japanese patients suspected of Hymenoptera venom allergy

    Directory of Open Access Journals (Sweden)

    Naruo Yoshida

    2015-07-01

    Conclusions: The measurement of sIgE following spiking of rVes v 5 and rPol d 5 by conventional testing in Japanese subjects with sIgE against hornet and paper wasp venom, respectively, improved the sensitivity for detecting Hymenoptera venom allergy. Improvement testing for measuring sIgE levels against hornet and paper wasp venom has potential for serologically elucidating Hymenoptera allergy in Japan.

  19. A new species of genus Chorebus Haliday (Hymenoptera, Alysiinae parasitising Hexomyza caraganae Gu (Diptera, Agromyzidae from NW China

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    Tao Li

    2017-03-01

    Full Text Available Chorebus (Stiphrocera hexomyzae sp. n. (Hymenoptera, Braconidae, Alysiinae, Dacnusini is described and illustrated. It was reared from twig galls of Hexomyza caraganae Gu (Diptera, Agromyzidae on Caragana korshinskii Kom. f. (Fabaceae in Ningxia and Inner Mongolia (NW China. A partial key to related or similar Chorebus species is provided.

  20. Preliminary taxonomic study of the genus Praon (Hymenoptera:Braconidae:Aphidiinae) and its host associations in Iran

    Czech Academy of Sciences Publication Activity Database

    Rakhshani, E.; Talebi, A. A.; Manzari, S.; Tomanovic, Ž.; Starý, Petr; Rezwani, A.

    2007-01-01

    Roč. 26, č. 2 (2007), s. 19-34 ISSN 0259-9996 R&D Projects: GA AV ČR IBS5007102 Institutional research plan: CEZ:AV0Z50070508 Keywords : genus Praon * Hymenoptera * Iran Subject RIV: EH - Ecology, Behaviour

  1. Temperature dependent functional response of Diaeretiella rapae (Hymenoptera: Aphidiidae) to the cabbage aphid, Brevicoryne brassicae (Hemiptera: Aphididae)

    DEFF Research Database (Denmark)

    Moayeri, Hamid R. S.; Madadi, Hossein; Pouraskari, Hossein

    2013-01-01

    Diaeretiella rapae MacIntosh (Hymenoptera: Aphidiidae) is one of the most common and successful parasitoids of the cabbage aphid. The functional response of D. rapae towards cabbage aphids was examined in laboratory studies at three constant temperatures, 17°C, 25°C and 30°C. D. rapae exhibited a...

  2. Biological parameters and thermal requirements of the parasitoid Praon volucre (Hymenoptera: Braconidae) with Macrosiphum euphorbiae (Hemiptera: Aphididae) as host

    NARCIS (Netherlands)

    Conti, De B.F.; Bueno, V.H.P.; Sampaio, M.V.; Lenteren, van J.C.

    2011-01-01

    The effect of temperature on the biology of Praon volucre (Haliday, 1833) (Hymenoptera: Braconidae) in Macrosiphum euphorbiae (Thomas, 1878) (Hemiptera: Aphididae) hosts was studied and the thermal requirements of the parasitoid were determined. Experiments were carried out at 16, 19, 22, 25, and 28

  3. A new species of Tamarixia Mercet (Hymenoptera, Eulophidae, parasitoid of Trioza aguacate Hollis & Martin (Hemiptera, Triozidae in Mexico

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    Zoya Yefremova

    2014-01-01

    Full Text Available Tamarixia aguacatensis Yefremova, sp. n. (Hymenoptera: Eulophidae: Tetrastichinae is described from Mexico as a parasitoid of the avocado psyllid, Trioza aguacate Hollis & Martin (Hemiptera: Triozidae. Trioza aguacate is a serious pest of avocado, Persea americana Miller. A key to the species of Tamarixia Mercet in Mexico is given.

  4. Sex determination in the haplodiploid wasp Nasonia vitripennis (Hymenoptera Chalcidoidea) : A critical consideration of models and evidence

    NARCIS (Netherlands)

    Beukeboom, Leo W.; Kamping, Albert; van de Zande, Louis

    Sex determining mechanisms are highly diverse. Like all Hymenoptera, the parasitic wasp Nasonia vitripennis reproduces by haplodiploidy: males are haploid and females are diploid. Sex in Nasonia is not determined by complementary alleles at sex loci. Evidence for several alternative models is

  5. Record of the genus Arrhenophagoidea Girault (Hymenoptera: Chalcidoidea: Encyrtidae from India, description of a new species from the Andaman Islands

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    M. Hayat

    2014-05-01

    Full Text Available The genus Arrhenophagoidea Girault (Hymenoptera: Encyrtidae is recorded for the first time from India and the Oriental region, and a new species, A. andamanica sp. Nov. is described from material collected in the Andaman and Nicobar Islands, India. As the genus is newly recorded from the Oriental region, a brief diagnosis is also given.

  6. A new species of the Camponotus aureopilus VIEHMEYER, 1914 species-group (Hymenoptera: Formicidae) from Papua New Guinea

    Czech Academy of Sciences Publication Activity Database

    Shattuck, S.; Janda, Milan

    2009-01-01

    Roč. 12, - (2009), s. 251-253 ISSN 1994-4136 R&D Projects: GA MŠk LC06073; GA AV ČR KJB612230701 Grant - others:U.S. National Science Foundation(US) DEB-02-11591 Institutional research plan: CEZ:AV0Z50070508 Keywords : taxonomic description * Hymenoptera * Formicidae Subject RIV: EG - Zoology

  7. A new species of Megischus Brullé (Hymenoptera, Stephanidae from China, with a key to the Chinese species

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    Hong Chun-dan

    2010-11-01

    Full Text Available A new species of Megischus Brullé from China, M. aplicatus sp. n., is described and illustrated. A key to the Chinese species of Megischus is added. The holotype is deposited in the Parasitic Hymenoptera Collection of Zhejiang University, Hangzhou.

  8. Two new species of Oobius (Hymenoptera: Encyrtidae) and their phylogenetic relationship with other congeners from northeastern Asia

    Science.gov (United States)

    Yan-Xia Yao; Jian J. Duan; Jason L. Mottern; Xiao-Yi Wang; Zhong-Qi Yang; Leah S. Bauer; Michael W. Gates

    2018-01-01

    Two new species of egg parasitoids, Oobius saimaensis Yao and Mottern new species and Oobius fleischeri Yao and Duan new species (Hymenoptera: Encyrtidae), are described from eggs of Agrilus fleischeri Obenberger, 1925 (Coleoptera: Buprestidae). Agrilus fleischeri is a phloemfeeding woodborer of poplar (...

  9. The parasites of cereal stem borers (Lepidoptera: Cossidae, Crambidae, Noctuidae, Pyralidae) in Africa, belonging to the family Braconidae (Hymenoptera: Ichneumonoidea)

    NARCIS (Netherlands)

    Achterberg, van C.; Polaszek, A.

    1996-01-01

    A review is given of the parasites (parasitoids) of the African cereal stem borers (including introduced species) belonging to the family Braconidae (Hymenoptera); 38 species belonging to 19 genera are keyed and treated. Three new species are described: Macrocentrus sesamivorus spec. nov. from

  10. New species of Megastylus (Hymenoptera: Ichneumonidae: Orthocentrinae) reared from larvae of Keroplatidae fungus gnats (Diptera) in a Dutch orchid greenhouse

    NARCIS (Netherlands)

    Humala, Andrei E.; Kruidhof, Marjolein; Woelke, Joop

    2017-01-01

    A new parasitoid wasp species belonging to the genus Megastylus (Hymenoptera: Ichneumonidae: Orthocentrinae) found in an orchid nursery in The Netherlands is described and illustrated: Megastylus woelkei sp. nov. It was reared from parasitized larvae of fungus gnats (Diptera: Keroplatidae). The

  11. An epidemiological survey of hymenoptera venom allergy in the Spanish paediatric population.

    Science.gov (United States)

    Martínez-Cañavate, A; Tabar, A I; Eseverri, J L; Martín, F; Pedemonte-Marco, C

    2010-01-01

    Hypersensitivity reactions to hymenoptera venom are infrequent in paediatric patients. A study was made to determine the incidence of this pathology in children, based on an epidemiological survey targeted to all members of the SEICAP (Sociedad Española de Inmunología Clínica y Alergia Pediátrica/Spanish Society of Paediatric Clinical Immunology and Allergy), and designed to collect the data on patients under 17 years of age diagnosed with hymenoptera venom allergy. The data corresponding to 175 patients (135 males) were collected. The mean age was 9.9 ± 3.6 years. Seventeen percent (32 patients) were the offspring of beekeepers, and 68.9% had experienced previous stings. The causal insect was Apis melifera, implicated in 55 cases, followed by Polistes dominulus (33 cases). In 151 patients (83.9%) the condition consisted of a local reaction. The most frequent systemic response was urticaria and angio-oedema. Fourteen patients suffered anaphylactic shock. The diagnosis was based on skin test (intradermal and prick) and/or specific IgE testing. Three treatment categories were established: (a) prevention and educational measures; (b) symptomatic treatment with oral antihistamines as well as self-injectable adrenalin; and (c) immunotherapy. In this context, 135 patients underwent immunotherapy with a mean duration of 3.5 ± 1.7 years (range 2-5 years) - with excellent tolerance. The starting regimen was predominantly conventional (92 patients). The results of this survey show hypersensitivity reactions to hymenoptera venom to be infrequent in paediatrics, though with a strong impact upon patient quality of life. Copyright © 2009 SEICAP. Published by Elsevier Espana. All rights reserved.

  12. Propriedades físicas e mecânicas de painéis LVL de Eucalyptus grandis

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    Hernando Alfonso Lara Palma

    2011-01-01

    Full Text Available O presente trabalho teve como objetivo principal avaliar as propriedades físicas e mecânicas de painéis LVL (Laminated veneer lumber confeccionados com lâminas de Eucalyptus grandis, provenientes de reflorestamentos da região de Sengés, Paraná - Brasil. Foram confeccionados painéis LVL de 23 lâminas, de dimensões comerciais de 2500 mm de comprimento, 1200 mm de largura e espessura nominal de 50 mm (lâminas de 2,4 mm. Em vigas retiradas dos painéis LVL foram analisadas as propriedades de flexão estática (rigidez e resistência, nas condições de menor inércia (flatwise e de maior inércia (edgewise, compressão paralela, cisalhamento paralelo nos planos L-X e L-Y e densidade, atendendo às prescrições das normas ASTM-D 5456/4761 e ASTM-D 198/143. Os valores médios obtidos para o módulo de elasticidade e resistência à flexão estática foram, respectivamente, 13114 MPa e 88,76 MPa para o ensaio na condição de menor inércia e de, respectivamente, 15871 MPa e 88,63 MPa para o ensaio na condição de maior inércia. A densidade aparente (a 12% de umidade dos painéis LVL e das lâminas foram, respectivamente, 690 kg/ m3 e 649 kg/m3. Os valores médios obtidos nos ensaios de compressão paralela foram 16856 MPa para o módulo de elasticidade e 58,05 MPa para a resistência. Os valores médios da resistência no ensaio de cisalhamento paralelo no plano L-Y e no plano L-X foram 5,96 MPa e 5,91 MPa, respectivamente. Todos esses valores atingiram parcialmente ou ultrapassaram os limites médios de referência estabelecidos para painéis LVL e para a madeira sólida originária, atestando, sobretudo, a qualidade dos painéis produzidos com essa madeira.

  13. Yields and nutrient pools in soils cultivated with Tectona grandis and Gmelina arborea in Nigerian rainforest ecosystem

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    V.A.J. Adekunle

    2011-06-01

    Full Text Available This study examined the yield of the two most prominent exotic species in southwest Nigeria and the nutrient status of soils cultivated with these species. The impacts of plantation development on soil nutrients were also examined. The plantations species are Gmelina arborea (Gmelina stands established in 1984, 1988, 1990 and 1994 and Tectona grandis (Teak established in 1990, 1992, 1994, 1996 and 1997. Growth data and composite soil samples from 3 depths (0–15, 15–30 and 30–60 cm were collected from five equal sized plots (20 × 20 m2 randomly located in the plantation of the two species. Also, soil samples were collected from the adjacent natural forest for comparison. The results for both species show that tree growth variables increased substantially with increase in tree age. There was significant difference in number of trees per hectare, dominant diameter, volume/ha and MAI for the Gmelina stands. In the Teak stand, there was significant difference in most of the tree growth variables also. Nutrients required by plants to survive were present in the soil samples from the plantations and the natural forest in different proportions. There was high correlation between percentage sand and most of the tree growth variables for both species. The pH value obtained for the Gmelina stands ranged between 6.47 and 7.47 while that of Teak stands ranged between 5.57 and 8.33. There was also a high and positive relationship between some soil chemical properties and tree growth variables. The highest significant correlation coefficient existed between phosphorus concentration and basal area for stands of both species. The r-values are 0.98 and 0.96 for Gmelina and Teak, respectively. While a high, negative and significant r-value (−0.88 was also obtained between potassium and volume/ha for the Gmelina stands, a high positive r-value was obtained between the potassium and basal area for the Teak stands. Comparison of soil nutrients in the

  14. A new candidate of calcium channel blocker in silico from Tectona grandis for treatment of gestational hypertension

    Science.gov (United States)

    Azizah, A.; Suselo, Y. H.; Muthmainah, M.; Indarto, D.

    2018-05-01

    grandis. Obtusifolin 2-glucoside computationally becomes a potensial candidate of calcium channel blocker. In vitro assays should be performed to evaluate the antagonist effect of obtusifolin 2-glucoside on calcium channel Cav1.2.

  15. Hymenoptera of Afghanistan and the central command area of operations: assessing the threat to deployed U.S. service members with insect venom hypersensitivity.

    Science.gov (United States)

    Turbyville, Joseph C; Dunford, James C; Nelson, Michael R

    2013-01-01

    Insect venom hypersensitivity can pose a threat to personnel deployed to a combat zone but the exposure risk in Afghanistan is currently unknown. This study was designed to assess the threat of Hymenoptera stings and associated allergic reactions in Afghanistan. Hymenoptera species were collected during a deployment to southern Afghanistan from June 2010 through January 2011. The literature was also reviewed to determine species of medically important Hymenoptera recorded in the region. The U.S. Army theater electronic medical data system was mined for ICD-9 codes associated with insect stings to determine the number of theater medical clinic encounters addressing insect sting reactions. Three species of flying hymenoptera were commonly encountered during the study period: Vespa orientalis L., Polistes wattii Cameron, and Vespula germanica (F.). A literature review also confirms the presence of honeybees (Apidae), numerous velvet ant (Mutillidae) species, and various ant (Formicidae) species all capable of stinging. No evidence was identified to suggest that fire ants (Solenopsis ssp.) are a threat in the region. Based on electronic medical records from the U.S. Central Command area of operations over a 2-year period, roughly 1 in 500 clinic visits involved a patient with a diagnosis of insect bite or sting. Cross-reactive members of all five flying Hymenoptera species commonly assessed for in Hymenoptera allergy evaluations are present in Afghanistan. The review of in-theater medical records confirms that insect stings pose an environmental threat to deployed service members.

  16. PERBANDINGAN KEANEKARAGAMAN HYMENOPTERA PARASITOID PADA AGROEKOSISTEM KEDELAI DENGAN APLIKASI DAN TANPA APLIKASI INSEKTISIDA

    Directory of Open Access Journals (Sweden)

    Hendrival Hendrival

    2017-04-01

    Full Text Available Abstrak Sistem pengelolaan tanaman kedelai dengan penggunaan insektisida sintetik yang intensif akan menurunkan keanekaragaman jenis Hymenoptera parasitoid. Penelitian bertujuan untuk memban-dingkan keanekaragaman Hymenoptera parasitoid pada agroekosistem kedelai dengan dan tanpa aplikasi insektisida sintetik. Pengumpulan data serangga menggunakan perangkap dari jaring serangga dan nampan kuning. Hasil penelitian menunjukkan bahwa indeks keanekaragaman jenis pada fase pertumbuhan vegetatif dan generatif kedelai dengan aplikasi insektisida lebih rendah dibandingkan dengan yang tanpa aplikasi insektisida, yang keduanya tergolong sedang. Indeks kemerataan jenis pada fase pertumbuhan vegetatif dan generatif dari kedua agroekosistem kedelai tergolong tinggi. Indeks kekayaan jenis pada fase vegetatif dari agroekosistem kedelai dengan aplikasi insektisida tergolong rendah (0<2,3955≤2,5, sedangkan pada fase generatif tergolong sedang (0<3,6118≤4. Indeks kekayaan jenis pada fase vegetatif (0<2,6229≤4 dan generatif (0<3,8287≤4 dari agroekosistem kedelai tanpa aplikasi insektisida tergolong sedang. Komunitas Hymenoptera parasitoid pada agroekosistem kedelai tanpa aplikasi insektisida memiliki kemiripan lebih rendah daripada yang dengan aplikasi insektisida. Aplikasi insektisida mempengaruhi indeks kekayaan jenis pada fase generatif dan kemiripan komunitasnya, yaitu nilainya lebih rendah daripada yang tanpa insektisida. Abstract The management system of soybean agroecosystem with an intensive use of synthetic insecticides will reduce the diversity of parasitoid Hymenoptera species. The study aimed to compare the diversity of the parasitoids in soybean agroecosystem with and without insecticide application. The collection of the parasitoid used insect net and yellow tray. The results showed that the diversity index of the parasitoids during vegetative and generative growth of the soybean with the insecticide application was lower than the one without

  17. Evolution of Cuticular Hydrocarbons in the Hymenoptera: a Meta-Analysis.

    Science.gov (United States)

    Kather, Ricarda; Martin, Stephen J

    2015-10-01

    Chemical communication is the oldest form of communication, spreading across all forms of life. In insects, cuticular hydrocarbons (CHC) function as chemical cues for the recognition of mates, species, and nest-mates in social insects. Although much is known about the function of individual hydrocarbons and their biosynthesis, a phylogenetic overview is lacking. Here, we review the CHC profiles of 241 species of Hymenoptera, one of the largest and most important insect orders, which includes the Symphyta (sawflies), the polyphyletic Parasitica (parasitoid wasps), and the Aculeata (wasps, bees, and ants). We investigated whether these taxonomic groups differed in the presence and absence of CHC classes and whether the sociality of a species (solitarily vs. social) had an effect on CHC profile complexity. We found that the main CHC classes (i.e., n-alkanes, alkenes, and methylalkanes) were all present early in the evolutionary history of the Hymenoptera, as evidenced by their presence in ancient Symphyta and primitive Parasitica wasps. Throughout all groups within the Hymenoptera, the more complex a CHC the fewer species that produce it, which may reflect the Occam's razor principle that insects' only biosynthesize the most simple compound that fulfil its needs. Surprisingly, there was no difference in the complexity of CHC profiles between social and solitary species, with some of the most complex CHC profiles belonging to the Parasitica. This profile complexity has been maintained in the ants, but some specialization in biosynthetic pathways has led to a simplification of profiles in the aculeate wasps and bees. The absence of CHC classes in some taxa or species may be due to gene silencing or down-regulation rather than gene loss, as demonstrated by sister species having highly divergent CHC profiles, and cannot be predicted by their phylogenetic history. The presence of highly complex CHC profiles prior to the vast radiation of the social Hymenoptera indicates a

  18. Taxonomic and faunistic study of Aulacidae (Hymenoptera, Evanioidea from Iran, with illustrated key to species

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    Mostafa Ghafouri Moghaddam

    2018-02-01

    Full Text Available Aulacidae are parasitoids of wood-boring larvae of Hymenoptera and Coleoptera, known in all zoogeographic regions of the World, except Antarctic. Two aulacids, Pristaulacus compressus (Spinola, 1808 and the rare Pristaulacus mourguesi Maneval, 1935, have been recently collected from Iran, the latter being a new record. Based on available data, the Iranian aulacid fauna includes five species within a single genus, Pristaulacus Kieffer 1900. A brief taxonomic treatment, as well as morphometric data and an illustrated key to species, are provided.

  19. Contribution to Taxonomy and Distribution of the Genus Elaphropoda (Hymenoptera: Apidae: Apinae in Vietnam

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    Nguyen, Minh Phuong

    2016-04-01

    Full Text Available Taxonomic notes on the genus Elaphropoda Lieftinck, 1966 (Hymenoptera: Apidae from Vietnam are presented. Two species of the genus are reported: Elaphropoda percarinata (Cockerell, 1930 was first recorded from Vietnam based on specimens collected from Ha Tinh province in 1998, and is reconfirmed with a specimens collected from Bac Kan province in the Northeastern part of the country in this study, and Elaphropoda khasiana (Schulz, 1906 is recorded from Vietnam for the first time. Redescriptions of the male of E. percarinata and the female of E. khasiana are given with illustrations.

  20. Utility of laboratory testing for the diagnosis of Hymenoptera venom allergy.

    Science.gov (United States)

    Vachová, Martina; Panzner, Petr; Malkusová, Ivana; Hanzlíková, Jana; Vlas, Tomáš

    2016-05-01

    A diagnosis of Hymenoptera venom allergy is based on clinical history and the results of skin tests and/or laboratory methods. To analyze the utility of available laboratory tests in diagnosing Hymenoptera venom allergy. Ninety-five patients with Hymenoptera venom allergy with a history of bee (35) or wasp (60) anaphylactic sting reaction and positive skin test with bee or wasp venom were included in this analysis. Specific immunoglobulin E (to bee venom extract, wasp venom extract, available recombinant molecules, and a basophil activation test with venom extracts were assessed in all the patients. Test sensitivity and specificity were calculated by using standard threshold values; then, receiver operating characteristic curve analysis was performed to compute optimal threshold values. Also, statistical analysis of the utility of different combinations of laboratory tests was performed. The optimal threshold values were revealed to be the following: 1.0 kIU/L for bee venom extract (sensitivity, 97.14%; specificity, 100%), 0.35 kIU/L for rApi m 1 (sensitivity, 68.57%; specificity, 100%), 1.22 kIU/L for wasp venom extract (sensitivity, 88.33%; specificity, 95.45%), 0.7 kIU/L for rVes v 5 (sensitivity, 86.67%; specificity, 95.45%), 1.0 kIU/L for rVes v 1 (sensitivity, 56.67%; specificity, 95.45%), 6.5% for basophil activation test with bee venom extract (sensitivity, 80%; specificity, 95.45%), and 4.5% for basophil activation test with wasp venom extract (sensitivity, 91.53%; specificity, 95.45%). The best test combinations were found to be the following: bee venom extract plus rApi m 1 (sensitivity, 97.14%; specificity, 95.45%) in bee and either wasp venom extract plus rVes v 5, or rVes v 5 plus rVes v 1 (both sensitivity, 98.33%; specificity, 95.45%) in patients with wasp venom allergy. Our analysis confirmed that currently used laboratory tests represent effective tools in diagnosing Hymenoptera venom allergy. Moreover, our probabilistic approach offered another

  1. Pollinator diversity (Hymenoptera and Diptera in semi-natural habitats in Serbia during summer

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    Mudri-Stojnić Sonja

    2012-01-01

    Full Text Available The aim of this study was to assess species diversity and population abundance of the two main orders of pollinating insects, Hymenoptera and Diptera. The survey was conducted in 16 grassland fragments within agro-ecosystems in Vojvodina, as well as in surrounding fields with mass-flowering crops. Pollinators were identified and the Shannon-Wiener Diversity Index was used to measure their diversity. Five families, 7 subfamilies, 26 genera and 63 species of insects were recorded. All four big pollinator groups investigated were recorded; hoverflies were the most abundant with 32% of the total number of individuals, followed by wild bees - 29%, honeybees - 23% and bumblebees with 16%.

  2. Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae

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    Danúncia Urban

    2011-06-01

    Full Text Available Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae. Melostelis gen. nov. é proposto para um novo Anthidiini cleptoparasita. São descritas e ilustradas duas espécies novas: Melostelis amazonensis sp. nov. de Manaus, Amazonas e Larocanthidium chacoense sp. nov. de Porto Murtinho, Mato Grosso do Sul. São dados a conhecer os machos de Epanthidium bolivianum Urban, 1995 e Epanthidium araranguense Urban, 2006 e, registrados pela primeira vez no Brasil, na sub-região do chaco, Ketianthidium zanolae Urban, 2000 e Epanthidium bolivianum.

  3. A new species of Eufriesea Cockerell (Hymenoptera, Apidae, Euglossina from northeastern Brazil

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    Luiz R. R. Faria

    2011-03-01

    Full Text Available A new species of Eufriesea Cockerell (Hymenoptera, Apidae from northeastern Brazil. Eufriesea pyrrhopyga sp. nov. a short-tongued Eufriesea is described as a new species. It can be easily recognized for its predominantly violet lower frons and thorax, violet tergum 1 contrasting with the strong reddish coloration on the lateral portions of terga 2 to 4 and on entire terga 5 and 6, and head pubescence with contrasting colors, white on the lower two-thirds of the face and black on upper frons and vertex. This new species, collected in Recife (Pernambuco, Brazil, apparently is restricted to the Pernambuco endemic center, and seems to be highly endangered.

  4. Five new species of Meteorus Haliday (Hymenoptera: Braconidae: Euphorinae) from Brazil.

    Science.gov (United States)

    De Almeida, Luis Felipe Ventura; Dias, Angélica Maria Penteado

    2015-12-10

    Meteorus Haliday, 1835 (Hymenoptera: Braconidae) is a cosmopolitan genus with around 340 species described, all koinobiont endoparasitoids of Coleoptera or Lepidoptera larvae, and several of its hosts are pest insects. Previously to this work only two species were described from Brazil, M. eaclidis Muesebeck and M. townsendi Muesebeck. Five new species of Meteorus are here described: M. atlanticus n. sp., M. ferruginosus n. sp., M. itatiaiensis n. sp., M. monoceros n. sp., and M. strigatus n. sp. Three species are recorded for the first time from Brazil: M. jerodi Aguirre & Shaw, M. laphygmae Viereck and M. megalops Zitani.

  5. Ciclo de desarrollo de Trigona (Tetragonisca angustula, Latreille 1811 (Hymenoptera, Trigonini

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    María Giomar Nates Parra

    1989-01-01

    Full Text Available Se estudió el ciclo de desarrollo de una abeja sin aguijón: Trigona (Tetragonisca angustula. Latreille 1811 (Hymenoptera. Trigonini. Los resultados fueron los siguientes: El periodo comprendido entre la postura del huevo y la emergencia del imago, es, en las obreras, de 36.5 días en promedio. La duración del ciclo se hace mayor a medida que las celdas se alejan del centro del panal. Se determinaron 3 instares larvales y 5 fases pupales para obreras.

  6. Dopluise (Hemiptera: Coccoidea geassosieer met die wipstertmier, Crematogaster peringueyi Emery (Hymenoptera: Formicidae

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    Johannes H. Giliomee

    2015-02-01

    Full Text Available Neste van die wipstertmier, Crematogaster peringueyi (Hymenoptera: Formicidae, is op verskeie plekke langs die kus van die Wes-Kaap versamel. Die doel was om vas te stel watter dopluisagtiges (Hemiptera: Coccoidea in die neste in assosiasie met hierdie miere leef. Dopluise van drie families, naamlik die Pseudococcidae (witluise, Coccidae (sagtedopluise en Kerriidae (lakdopluise is in die neste gevind, almal bekend daarvoor dat hulle heuningdou afskei. Hierdie mutualistiese verhouding tussen die miere en dopluise, bekend as mirmekofilie, is fakultatief van aard. Die wipstertmier blyk ook nie spesifiek te wees wat betref die plant waarop hulle nes maak nie.

  7. TRAP-NESTING BEES AND WASPS (HYMENOPTERA, ACULEATA IN A SEMIDECIDUAL SEASONAL FOREST FRAGMENT, SOUTHERN BRAZIL

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    PRISCILA S. OLIVEIRA

    Full Text Available ABSTRACT Trap-nesting bee and wasp inventories are common in Brazil but many phytophysiognomies are still poorly studied. The main objective of this study is to survey trap-nesting bees and wasps in a Semidecidual Seasonal Forest fragment. Also, we test the differences on nesting between interior and edge transects. A sum of 1,500 trap nests was made with bamboo cane internodes and two consecutive years were monitored. In the first year 46 nests were occupied by Pachodynerus grandis (19 nests, Pachodynerus guadulpensis (19, Centris analis (two, and Centris tarsata, Megachile fiebrigi, Megachile guaranitica, Megachile susurrans, Trypoxylon sp and Zethus smithii with one nest each. No statistical differences were found between interior and edge transects for richness and occupation rate, but the species composition was different. In the second year 39 nests were occupied by four species, three previously recorded, C. analis (seven nests, P. guadulpensis and P. grandis (six nests each, plus Monobia angulosa with 15 nests. Parasitoids from four families and one cleptoparasite were recorded and the mortality rate was higher in bees than in wasps. These findings reinforce the notion that trap nests assemblages from different studies are not directly comparable for richness and composition.

  8. Larvae and Nests of Aculeate Hymenoptera (Hymenoptera: Aculeata) Nesting in Reed Galls Induced by Lipara spp. (Diptera: Chloropidae) with a Review of Species Recorded. Part II.

    Science.gov (United States)

    Astapenková, Alena; Heneberg, Petr; Bogusch, Petr

    2017-01-01

    The ability of aculeate Hymenoptera to utilize wetlands is poorly understood, and descriptions of their nests and developmental stages are largely absent. Here we present results based on our survey of hymenopterans using galls induced by Lipara spp. flies on common reed Phragmites australis in the years 2015-2016. We studied 20,704 galls, of which 9,446 were longitudinally cut and the brood from them reared in the laboratory, while the remaining 11,258 galls reared in rearing bags also in laboratory conditions. We recorded eight species that were previously not known to nest in reed galls: cuckoo wasps Chrysis rutilans and Trichrysis pumilionis, solitary wasps Stenodynerus chevrieranus and Stenodynerus clypeopictus, and bees Pseudoanthidium tenellum, Stelis punctulatissima, Hylaeus communis and Hylaeus confusus. Forty five species of Hymenoptera: Aculeata are known to be associated with reed galls, of which 36 make their nests there, and the other are six parasitoids of the family Chrysididae and three cuckoo bees of the genus Stelis. Of these species, Pemphredon fabricii and in southern Europe also Heriades rubicola are very common in reed galls, followed by Hylaeus pectoralis and two species of the genus Trypoxylon. We also found new host-parasite associations: Chrysis angustula in nests of Pemphredon fabricii, Chrysis rutilans in nests of Stenodynerus clypeopictus, Trichrysis pumilionis in nests of Trypoxylon deceptorium, and Stelis breviuscula in nests of Heriades rubicola. We provide new descriptions of the nests of seven species nesting in reed galls and morphology of mature larvae of eight species nesting in reed galls and two parasitoids and one nest cleptoparasite. The larvae are usually very similar to those of related species but possess characteristics that make them easy to distinguish from related species. Our results show that common reeds are not only expansive and harmful, but very important for many insect species associated with habitats

  9. Spatial distribution of adult Anthonomus grandis Boheman (Coleoptera: Curculionidae and buds with feeding punctures on conventional and Bt cottonDistribuição espacial de adultos e botões com orifício de alimentação de Anthonomus grandis Boheman (Coleoptera: Curculionidae em algodoeiro convencional e Bt

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    Paulo Rogerio Beltramin da Fonseca

    2013-06-01

    Full Text Available O conhecimento dos arranjos de dispersão para adultos e botões com orifício de alimentação de Anthonomus grandis em cultivares de algodoeiro é necessário para aperfeiçoar o monitoramento e controle da praga. Esta pesquisa teve por objetivo realizar análises probabilísticas dos padrões de distribuição espacial dos adultos e botões com orifícios de alimentação de A. grandis em duas cultivares de algodão Bt e não Bt. O estudo foi conduzido a campo em duas áreas experimentais, cada uma composta por 100 parcelas de sete linhas de sete metros de comprimento. Em 16 amostragens avaliaram-se cinco plantas por parcela através da contagem dos adultos e dos botões com orifício de alimentaçãoentre janeiro e maio de 2010. Foram calculados os índices de dispersão (razão variância/média, índice de Morisita e Expoente k da Distribuição Binomial Negativa e as distribuições teóricas de freqüência (Poisson, Binomial Negativa e Binomial Positiva. Não houve diferença estatística entre as cultivares avaliadas. A distribuição espacial dos adultos de A. grandis, nas cultivares Bt e não Bt, ajustou-se nos arranjos probabilísticos de distribuição binomial negativa (agregado e distribuição binomial positiva (uniforme, conforme os dias após a emergência do algodoeiro. As análises de dispersão para os botões com orifícios de alimentação nas culturas Bt e convencional mostraram os modelos espaciais de Poisson (aleatório, distribuição binomial negativa (agregado e distribuição binomial positiva (uniforme, em seqüência, durante o ciclo da cultura.Dispersion patterns of Anthonomus grandis adults and damaged squares with their feeding punctures are important to enhance pest monitoring and control on cotton. In this research we performed probabilistic analyses of the distribution patterns of adults and squares with feeding punctures of A. grandis on two cotton genotypes, Bt and non-Bt near isogenic lines. We conducted

  10. Growth rate and ring width variability of teak, Tectona grandis (Verbenaceae in an unmanaged forest in East Timor

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    Vicelina B. Sousa

    2012-03-01

    Full Text Available Teak (Tectona grandis is one of the most valuable timbers in international trade and an important species for tropical forestry. Teak is found on the island of East Timor but no information is available on teak growth from this region. A pure stand planted in 1940-50 in the North of East Timor and left unmanaged was studied. Fifteen trees were sampled in October-November 2003 and stem discs taken at three height levels of its height (1.7m, 9.5m and 18.7m, and cores were collected at DBH. Transverse surfaces of the discs and cores were polished for ring identification. Core cross sections were first digitized and disc cross sections were observed under the microscope. Three randomly selected radii were analyzed in each disc. Ring width measurement and ring counting were done using image analysis software. The distinction between heartwood and sapwood was performed macroscopically by colour difference, and heartwood radius and sapwood width were measured. The relationship between stem and heartwood radius was studied for each disc and heartwood percentage by radius was determined. Radial ring width curves are presented for the different axial positions within the stem, and ring width variability was analyzed. Growth rates were calculated and age-radius relationships were estimated using cumulative growth curves. Growth rings were large and well defined in the juvenile phase, reflecting the specie’s fast-growing character. The year-to-year variation of ring width showed a similar pattern among trees. Mean ring width ranged between 4.3-7.3mm for the first 20 years and 3.3-5.1mm for 30 to 45 years. Pith eccentricity was evident in the lower part of the stem and ring wedging occurred. On average, heartwood represented 84% of the radius and sapwood contained 6 to 11 rings. The age-related variation of ring width and the occurrence in the lower part of the tree stems of eccentricity and wedging rings, highlights the importance of appropriate stand

  11. Método de diagnóstico para el monitoreo de resistencia a insecticidas en poblaciones de “picudo del algodonero”, Anthonomus grandis (Coleoptera: Curculionidae

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    Teodoro STADLER

    2009-01-01

    Full Text Available El control de las poblaciones de Anthonomus grandis Boheman, por debajo de su umbral de daño económico durante el ciclo del cultivo del algodón, se realiza en forma efectiva hasta el momento, a través de insecticidas de síntesis. La presión selectiva de las aplicaciones extensivas e intensivas de insecticidas hace imperativa la detección temprana de focos de resistencia a los mismos, en función de un correcto manejo del fenómeno. Se desarrolló un método de diagnóstico de resistencia para A. grandis a partir de la técnica “vial test”, que fue adaptada en forma de “kit” para el monitoreo rápido y sencillo de los focos de resistencia en el campo. La toxicidad (CL99, para calcular la concentración discriminante (CD del insecticida y la preparación del “kit”, se obtiene a partir de bioensayos de laboratorio con una cepa normal susceptible de A. grandis. Se determinó la vida media de los insecticidas dentro de los viales por CIPAC MT 46, para establecer una fecha de vencimiento del “kit”. La CD y el método en su conjunto fueron validados a través de ensayos a campo. El “kit”, usado en el monitoreo de resistencia en el campo, fue especialmente diseñado para ser utilizado en las condiciones geográficas, económicas y socio-culturales presentes en la región algodonera argentina. La implementación de esta técnica permitirá conseguir la información necesaria, y así obtener una apropiada alternancia de insecticidas. Como consecuencia, se prevé una reducción de impactoambiental de las prácticas agronómicas en el control de plagas en algodón.

  12. Qualidade de juntas coladas com lâminas de madeira oriundas de três regiões do tronco de Eucalyptus grandis, Eucalyptus saligna e Pinus elliottii Quality of wood joints glued with wood veneers from three trunk regions of Eucalyptus grandis, Eucalyptus saligna and Pinus elliottii

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    Benedito Rocha Vital

    2006-08-01

    Full Text Available Este experimento teve como objetivo avaliar a resistência de juntas coladas formadas pelas combinações de lâminas provenientes de três posições no tronco da madeira de Eucalyptus grandis, Eucalyptus saligna e Pinus elliottii. Foram empregados adesivos à base de poliacetato de vinila de média e alta viscosidade e resorcinol-formaldeído nas gramaturas de 150 g/m², em face simples para o poliacetato de vinila de média e alta viscosidades e 300 g/m², em face dupla, para o adesivo resorcinólico. O teor médio de umidade das lâminas, no momento da colagem, foi igual a 14%. Os valores médios mais elevados de resistência ao cisalhamento foram obtidos nas juntas produzidas com madeira de Eucalyptus saligna, coladas com adesivos de poliacetato de média viscosidade e resorcinol-formaldeído. A maior porcentagem de falha profunda na madeira foi obtida em juntas de madeira de Pinus elliottii, unidas com adesivo de poliacetato de alta viscosidade, seguidas das juntas de Eucalyptus grandis e coladas com adesivo de poliacetato de média viscosidade. As combinações de lâminas oriundas das seguintes posições no tronco: medula e casca, intermediária e casca e casca e casca resultaram em linhas de cola com maiores resistências ao cisalhamento.The objective of this work was to evaluate the shear strength of glued wood joints from pith, outer and intermediary wood of Eucalyptus saligna, Eucalyptus grandis and Pinus elliottii. High and medium viscosity polyvinyl acetate and resorcinol-phenol adhesives were applied at spread rate of 150 g/m² in single line and at spread rate of 300 g/m² in double glue line for the resorcinolic adhesive. The mean wood moisture content was 14%. Higher shear strength was obtained with Eucalyptus saligna veneer glued with medium viscosity polyvinyl resorcinolic adhesive. The highest percentage of wood failure was found on Pinus elliottii veneer glued with high viscosity polyvinyl acetate adhesive followed by

  13. Microbial biomass and activity in litter during the initial development of pure and mixed plantations of Eucalyptus grandis and Acacia mangium Biomassa e atividade microbiana da serapilheira durante o desenvolvimento inicial de plantios puros e mistos de Eucalyptus grandis e Acacia mangium

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    Daniel Bini

    2013-02-01

    Full Text Available Studies on microbial activity and biomass in forestry plantations often overlook the role of litter, typically focusing instead on soil nutrient contents to explain plant and microorganism development. However, since the litter is a significant source of recycled nutrients that affect nutrient dynamics in the soil, litter composition may be more strongly correlated with forest growth and development than soil nutrient contents. This study aimed to test this hypothesis by examining correlations between soil C, N, and P; litter C, N, P, lignin content, and polyphenol content; and microbial biomass and activity in pure and mixed second-rotation plantations of Eucalyptus grandis and Acacia mangium before and after senescent leaf drop. The numbers of cultivable fungi and bacteria were also estimated. All properties were correlated with litter C, N, P, lignin and polyphenols, and with soil C and N. We found higher microbial activity (CO2 evolution in litter than in soil. In the E. grandis monoculture before senescent leaf drop, microbial biomass C was 46 % higher in litter than in soil. After leaf drop, this difference decreased to 16 %. In A. mangium plantations, however, microbial biomass C was lower in litter than in soil both before and after leaf drop. Microbial biomass N of litter was approximately 94 % greater than that of the soil in summer and winter in all plantations. The number of cultivable fungi and bacteria increased after leaf drop, especially so in the litter. Fungi were also more abundant in the E. grandis litter. In general, the A. mangium monoculture was associated with higher levels of litter lignin and N, especially after leaf drop. In contrast, the polyphenol and C levels in E. grandis monoculture litter were higher after leaf drop. These properties were negatively correlated with total soil C and N. Litter in the mixed stands had lower C:N and C:P ratios and higher N, P, and C levels in the microbial biomass. This suggests more

  14. Hornets Have It: A Conserved Olfactory Subsystem for Social Recognition in Hymenoptera?

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    Antoine Couto

    2017-06-01

    Full Text Available Eusocial Hymenoptera colonies are characterized by the presence of altruistic individuals, which rear their siblings instead of their own offspring. In the course of evolution, such sterile castes are thought to have emerged through the process of kin selection, altruistic traits being transmitted to following generation if they benefit relatives. By allowing kinship recognition, the detection of cuticular hydrocarbons (CHCs might be instrumental for kin selection. In carpenter ants, a female-specific olfactory subsystem processes CHC information through antennal detection by basiconic sensilla. It is still unclear if other families of eusocial Hymenoptera use the same subsystem for sensing CHCs. Here, we examined the existence of such a subsystem in Vespidae (using the hornet Vespa velutina, a family in which eusociality emerged independently of ants. The antennae of both males and female hornets contain large basiconic sensilla. Sensory neurons from the large basiconic sensilla exclusively project to a conspicuous cluster of small glomeruli in the antennal lobe, with anatomical and immunoreactive features that are strikingly similar to those of the ant CHC-sensitive subsystem. Extracellular electrophysiological recordings further show that sensory neurons within hornet basiconic sensilla preferentially respond to CHCs. Although this subsystem is not female-specific in hornets, the observed similarities with the olfactory system of ants are striking. They suggest that the basiconic sensilla subsystem could be an ancestral trait, which may have played a key role in the advent of eusociality in these hymenopteran families by allowing kin recognition and the production of altruistic behaviors toward relatives.

  15. Genes underlying reproductive division of labor in termites, with comparisons to social Hymenoptera

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    Judith eKorb

    2016-04-01

    Full Text Available All social insects are characterized by a reproductive division of labor. Within a colony only a few individuals reproduce (queens and in termites, also a king while the large majority (workers and soldiers forgo reproduction, at least temporarily. The evolution of such reproductive altruism can ultimately be explained by inclusive fitness theory. Here, I will review the proximate genetic mechanisms underlying this altruism in termites. As social cockroaches they evolved eusociality independently from the social Hymenoptera, which makes them interesting test cases to look for common underlying mechanisms of eusociality and lineage specific idiosyncrasies. First, I will provide a summary of the genes and their function that have been identified to underlie reproductive division of labor - so called 'queen genes,' - in the drywood termite Cryptotermes secundus, an emerging model to study termite social evolution. Second, I outline how widespread these queen genes are across the termite phylogeny, using also evidence from recent genome analyses. I will provide hypotheses about the evolutionary origin of these queen genes, aiming to link proximate mechanisms with ultimate functions. Finally, I will draw comparisons to social Hymenoptera to indicate potential common underpinnings that warrant further testing.

  16. A hymenopterists’ guide to the Hymenoptera Anatomy Ontology: utility, clarification, and future directions

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    Katja Seltmann

    2012-05-01

    Full Text Available Hymenoptera exhibit an incredible diversity of phenotypes, the result of ~240 million years of evolution and the primary subject of more than 250 years of research. Here we describe the history, development, and utility of the Hymenoptera Anatomy Ontology (HAO and its associated applications. These resources are designed to facilitate accessible and extensible research on hymenopteran phenotypes. Outreach with the hymenopterist community is of utmost importance to the HAO project, and this paper is a direct response to questions that arised from project workshops. In a concerted attempt to surmount barriers of understanding, especially regarding the format, utility, and development of the HAO, we discuss the roles of homology, “preferred terms”, and “structural equivalency”. We also outline the use of Universal Resource Identifiers (URIs and posit that they are a key element necessary for increasing the objectivity and repeatability of science that references hymenopteran anatomy. Pragmatically, we detail a mechanism (the “URI table” by which authors can use URIs to link their published text to the HAO, and we describe an associated tool (the “Analyzer” to derive these tables. These tools, and others, are available through the HAO Portal website (http://portal.hymao.org. We conclude by discussing the future of the HAO with respect to digital publication, cross-taxon ontology alignment, the advent of semantic phenotypes, and community-based curation.

  17. The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae, a parasitoid wasp of Drosophila

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    Daniel S. Oliveira

    Full Text Available Abstract The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively. The gene pattern revealed extensive rearrangements compared to the typical pattern generally observed in insects. These rearrangements involve two protein-coding and two ribosomal genes, along with the 16 tRNA genes. This gene order is different from the pattern described for Ibalia leucospoides (Ibaliidae, Cynipoidea, suggesting that this particular gene order can be variable among Cynipoidea superfamily members. A maximum likelihood phylogenetic analysis of the main groups of Apocrita was performed using amino acid sequence of 13 protein-coding genes, showing monophyly for the Cynipoidea superfamily within the Hymenoptera phylogeny.

  18. QUALIDADE DA CELULOSE KRAFT-ANTRAQUINONA DE Eucalyptus dunnii PLANTADO EM CINCO ESPAÇAMENTOS EM RELAÇÃO AO Eucalyptus grandis E Eucalyptus saligna

    OpenAIRE

    Maria Cladis Mezzomo da Silva; Edvins Ratnieks; Teotônio Francisco de Assis; Celso Edmundo B. Foelkel; Jorge Vieira Gonzaga; Giovanni Willer Ferreira

    1997-01-01

    Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m), para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m). Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraquinon...

  19. Feeding and oviposition deterrent activities of flower buds of globemallow,Sphaeralcea emoryi torrey, against boll weevil,Anthonomus grandis Boheman (Coleoptera: Curculionidae).

    Science.gov (United States)

    Honda, H; Bowers, W S

    1996-01-01

    The globemallow,Sphaeralcea emoryi Torrey, a plant native to Arizona was evaluated as a source of feeding or oviposition deterrents to the boll weevil,Anthonomus grandis Boheman. Feeding and oviposition responses of reproductive weevils to the flower buds and artificial diets spiked with dry powder or extracts of the globemallow buds were determined. Boll weevils were deterred from feeding and ovipositing in the flower buds unless the calyxes were removed. Male and virgin female weevils were discouraged from feeding as much as gravid weevils. Secondary chemicals in the flower buds served primarily as feeding deterrents but also prevented oviposition. The concentration of these chemicals was highest in the calyxes of the buds, and potent deterrent activity could be extracted from the calyxes with methanol. Boll weevils were able to perceive the deterrents by contact chemosensory organs on the antennae, maxillary palps and labial palps.

  20. Da Rabelais a Zola, da Erasmo a Camus: la psicologia del processo, le intuizioni dei grandi autori, la conferma delle scienze cognitive

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    Carlo Bona

    2012-06-01

    Full Text Available Rabelais, Zola, Camus hanno intuito che le sentenze spesso si reggono su complessi meccanismi psicologici, oltre che sulla razionalità giuridica. Ci si chiede se queste intuizioni trovino conferma nelle più recenti acquisizioni della psicologia della decisione e delle scienze cognitive. Per rispondere al quesito si analizzano passi del Gargantua e Pantagruele, di Thérèse Raquin, de Lo straniero, mettendoli a confronto con alcuni esperimenti condotti in ambito psicologico. Si dimostra come Rabelais abbia correttamente intuito quanto rilevino le euristiche nella decisione, come Zola abbia colto il ruolo delle aspettative nella percezione da parte dei testi, come Camus abbia descritto le distorsioni che si possono produrre nella fase narrativa della testimonianza. Se ne conclude che a volte la materia della giustizia si intende meglio esplorando le opere dei grandi autori che non, per dirla con Erasmo, ammucchiando glosse su glosse.

  1. Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda and cotton boll weevil (Anthonomus grandis

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    Raquel Sampaio Oliveira

    2016-02-01

    Full Text Available Gossypium hirsutum (commercial cooton is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. Several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including Cry toxins, proteinase inhibitors, and toxic peptides, among others. In the present study, we developed transgenic cotton plants by fertilized floral buds injection (through the pollen-tube pathway technique using an DNA expression cassette harboring the cry1Ia12 gene, driven by CaMV35S promoter. The T0 transgenic cotton plants were initially selected with kanamycin and posteriorly characterized with PCR and Southern blot experiments to confirm the genetic transformation. Western blot and ELISA assays indicated the transgenic cotton plants with higher Cry1Ia12 protein expression levels to be further tested in the control of two major G. hirsutum insect pests. Bioassays with T1 plants revealed the Cry1Ia12 protein toxicity on Spodoptera frugiperda larvae, as evidenced by mortality up to 40% and a significant delay in the development of the target insects compared to untransformed controls (up to 30-fold. Also, a significant reduction of Anthonomus grandis emerging adults (up to 60% was observed when the insect larvae were fed on T1 floral buds. All the larvae and adult insect survivors on the transgenic lines were weaker and significantly smaller compared to the non-transformed plants. Therefore, this study provides GM cotton plant with simultaneous resistance against the Lepidopteran (S. frugiperda and the Coleopteran (A. grandis insect orders, and all data suggested that the Cry1Ia12 toxin could effectively enhance the cotton transgenic plants resistance to both insect pests.

  2. Transgenic Cotton Plants Expressing Cry1Ia12 Toxin Confer Resistance to Fall Armyworm (Spodoptera frugiperda) and Cotton Boll Weevil (Anthonomus grandis).

    Science.gov (United States)

    de Oliveira, Raquel S; Oliveira-Neto, Osmundo B; Moura, Hudson F N; de Macedo, Leonardo L P; Arraes, Fabrício B M; Lucena, Wagner A; Lourenço-Tessutti, Isabela T; de Deus Barbosa, Aulus A; da Silva, Maria C M; Grossi-de-Sa, Maria F

    2016-01-01

    Gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. Several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including Cry toxins, proteinase inhibitors, and toxic peptides, among others. In the present study, we developed transgenic cotton plants by fertilized floral buds injection (through the pollen-tube pathway technique) using an DNA expression cassette harboring the cry1Ia12 gene, driven by CaMV35S promoter. The T0 transgenic cotton plants were initially selected with kanamycin and posteriorly characterized by PCR and Southern blot experiments to confirm the genetic transformation. Western blot and ELISA assays indicated the transgenic cotton plants with higher Cry1Ia12 protein expression levels to be further tested in the control of two major G. hirsutum insect pests. Bioassays with T1 plants revealed the Cry1Ia12 protein toxicity on Spodoptera frugiperda larvae, as evidenced by mortality up to 40% and a significant delay in the development of the target insects compared to untransformed controls (up to 30-fold). Also, an important reduction of Anthonomus grandis emerging adults (up to 60%) was observed when the insect larvae were fed on T1 floral buds. All the larvae and adult insect survivors on the transgenic lines were weaker and significantly smaller compared to the non-transformed plants. Therefore, this study provides GM cotton plant with simultaneous resistance against the Lepidopteran (S. frugiperda), and the Coleopteran (A. grandis) insect orders, and all data suggested that the Cry1Ia12 toxin could effectively enhance the cotton transgenic plants resistance to both insect pests.

  3. Transgenic Cotton Plants Expressing Cry1Ia12 Toxin Confer Resistance to Fall Armyworm (Spodoptera frugiperda) and Cotton Boll Weevil (Anthonomus grandis)

    Science.gov (United States)

    de Oliveira, Raquel S.; Oliveira-Neto, Osmundo B.; Moura, Hudson F. N.; de Macedo, Leonardo L. P.; Arraes, Fabrício B. M.; Lucena, Wagner A.; Lourenço-Tessutti, Isabela T.; de Deus Barbosa, Aulus A.; da Silva, Maria C. M.; Grossi-de-Sa, Maria F.

    2016-01-01

    Gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. Several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including Cry toxins, proteinase inhibitors, and toxic peptides, among others. In the present study, we developed transgenic cotton plants by fertilized floral buds injection (through the pollen-tube pathway technique) using an DNA expression cassette harboring the cry1Ia12 gene, driven by CaMV35S promoter. The T0 transgenic cotton plants were initially selected with kanamycin and posteriorly characterized by PCR and Southern blot experiments to confirm the genetic transformation. Western blot and ELISA assays indicated the transgenic cotton plants with higher Cry1Ia12 protein expression levels to be further tested in the control of two major G. hirsutum insect pests. Bioassays with T1 plants revealed the Cry1Ia12 protein toxicity on Spodoptera frugiperda larvae, as evidenced by mortality up to 40% and a significant delay in the development of the target insects compared to untransformed controls (up to 30-fold). Also, an important reduction of Anthonomus grandis emerging adults (up to 60%) was observed when the insect larvae were fed on T1 floral buds. All the larvae and adult insect survivors on the transgenic lines were weaker and significantly smaller compared to the non-transformed plants. Therefore, this study provides GM cotton plant with simultaneous resistance against the Lepidopteran (S. frugiperda), and the Coleopteran (A. grandis) insect orders, and all data suggested that the Cry1Ia12 toxin could effectively enhance the cotton transgenic plants resistance to both insect pests. PMID:26925081

  4. EMERGÊNCIA E DESENVOLVIMENTO INICIAL DE PLÂNTULAS DE Cassia grandis L. f. EM FUNÇÃO DE DIFERENTES SUBSTRATOS

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    Caio César Pereira Leal

    2016-01-01

    Full Text Available ABSTRACT Currently, it is aimed to use substrates composed primarily of organic waste in order to reduce costs with chemical fertilizer. In that sense, the experiment was to evaluate different types of substrates for the emergence and early development of seedlings of Cassia grandis L. f. The experiment was carried out in a greenhouse at the Departament of Vegetable Sciences of Federal Rural do Semi- Árido (UFERSA, in Mossoró, Rio Grande do Norte state, Brazil. The substrates used were: sand + carbonized rice husk (in ratios of 1:1, 1:3 and 3:1, plant sand + wood dust (in ratios of 1:1, 1:3 and 3:1, plant sand + coconut fiber (in ratios of 1:1, 1:3 and 3:1, plant sand + chestnut bark (in ratios of 1:1, 1:3 and 3:1 and plant sand (control. The experimental design was a completely randomized design with 13 treatments (combinations of substrates, with four replications of 25 seeds and the means to test for Skot-knot (pr <0.05. We evaluated the following variables: plant emergence, emergence speed, plant height, root length and weight of the aerial part of the plant, root dry mass and total dry mass. According to the results, the substrate plant sand + sawdust (1:1 and plant sand + coconut fiber (1:3 showed higher values, providing better results in emergence, weight of the aerial part of the plant, length of the aerial part of the plant, root dry mass and root length. It was found that the substrate compounds sand plant and chestnut bark (1:3 is harmful to the emergence of seedling of Cassia grandis .

  5. Subcellular partitioning of cadmium in the freshwater bivalve, Pyganodon grandis, after separate short-term exposures to waterborne or diet-borne metal

    Energy Technology Data Exchange (ETDEWEB)

    Cooper, Sophie; Hare, Landis [INRS-Eau, Terre et Environnement, Universite du Quebec, 490 rue de la Couronne, Quebec, QC, G1K 9A9 (Canada); Campbell, Peter G.C., E-mail: peter.campbell@ete.inrs.ca [INRS-Eau, Terre et Environnement, Universite du Quebec, 490 rue de la Couronne, Quebec, QC, G1K 9A9 (Canada)

    2010-11-15

    The dynamics of cadmium uptake and subcellular partitioning were studied in laboratory experiments conducted on Pyganodon grandis, a freshwater unionid bivalve that shows promise as a biomonitor for metal pollution. Bivalves were collected from an uncontaminated lake, allowed to acclimate to laboratory conditions ({>=}25 days), and then either exposed to a low, environmentally relevant, concentration of dissolved Cd (5 nM; 6, 12 and 24 h), or fed Cd-contaminated algae ({approx}70 nmol Cd g{sup -1} dry weight; 4 x 4 h). In this latter case, the bivalves were allowed to depurate for up to 8 days after the end of the feeding phase. As anticipated, the gills were the main target organ during the aqueous Cd exposure whereas the intestine was the initial site of Cd accumulation during the dietary exposure; during the subsequent depuration period, the dietary Cd accumulated in both the digestive gland and in the gills. For the gills, the distribution of Cd among the subcellular fractions (i.e., granules > heat-denatured proteins (HDP) {approx} heat-stable proteins (HSP) > mitochondria {approx} lysosomes + microsomes) was insensitive to the exposure route; both waterborne and diet-borne Cd ended up largely bound to the granule fraction. The subcellular distribution of Cd in the digestive gland differed markedly from that in the gills (HDP > HSP {approx} granules {approx} mitochondria > lysosomes + microsomes), but as in the case of the gills, this distribution was relatively insensitive to the exposure route. For both the gills and the digestive gland, the subcellular distributions of Cd differed from those observed in native bivalves that are chronically exposed to Cd in the field - in the short-term experimental exposures of P. grandis, metal detoxification was less effective than in chronically exposed native bivalves.

  6. Respuesta fotosintética de Eucalyptus grandis W. Hill a la disponibilidad de agua en el suelo y a la intensidad de luz

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    María Sara Mejía de Tafur

    2014-12-01

    Full Text Available En condiciones de campo en la Universidad Nacional de Colombia sede Palmira (950 m.s.n.m., 24°C y 75% de humedad relativa se evaluó la respuesta fotosintética de cuatro clones de Eucalyptus grandis (28-3, 18-3, 24A-5, 19-1 al estrés hídrico: capacidad de campo (1 cc, 0.5 cc, 0.25 cc y a la saturación de agua e intensidades lumínicas variables de 1500, 1000, 500, 250, y 0 µmol/m²/s en el primer ensayo y de 1500, 1000, 435, 87, 70, 52, 35, 26, y 0 µmol/m²/s en el segundo. En ambos estudios, se utilizó un diseño experimental de parcelas divididas con cuatro repeticiones, donde la parcela principal correspondió al nivel de humedad en el suelo y la subparcela a los clones. El análisis estadístico mostró que E. grandis reduce la tasa de fotosíntesis de manera significativa cuando se presenta estrés hídrico en el suelo y que el grado de la respuesta depende del clon, lo que sugiere la presencia de mecanismos fisiológicos dependientes del genotipo para responder al estrés hídrico. Se presentaron curvas de luz típicas con puntos de compensación bajos (0.9 - 7.0 µmol/m²/s, tasas de fotosíntesis altas (68 - 39 µmol CO2 /m²/s y alta eficiencia fotosintética (0.0003 - 0.0086 mol CO2 /mol luz

  7. INFLUÊNCIA DA TERMORRETIFICAÇÃO NA RESISTÊNCIA A DEGRADAÇÃO BIOLÓGICA DAS MADEIRAS DE Pinus taeda E Eucalyptus grandis

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    Karina Soares Modes

    2017-01-01

    Full Text Available This study investigated the effect of two thermal treatment technique on the biological properties of the wood of Pinus taeda L. and Eucalyptus grandis and W. Hill ex Maiden, comparing them with results obtained for the untreated wood. Of each species were sampled three trees at age 25, from where it drew a central plank height DBH for making test specimens were subjected to thermal treatments for later be transformed into pieces of 2.5 x 2.5 x 0.9 cm. In the first treatment the wood were subjected to thermal treatment by autoclaving at 130 ºC/ ± 3 ° C and pressure of 2 kgf /cm² for 3 hours and, after a conditioning period, subjected to heat in an electric oven at 160 °C/ ± 1 °C for the same period. The second treatment consisted of only heat treatment by kiln. Resistance to biological attack was evaluated by accelerated decay test in the laboratory, using a white-rot fungus, Trametes versicolor (Linnaeus ex Fries Pilat and other brown rot Gloeophyllum trabeum (Persoon. ex Fries Murr. The combined treatment of the autoclave with electric oven favored the decay of both species of wood fungi employees, except for Pinus taeda submitted to the fungus Trametes versicolor , while treatment in an oven provided increase biological resistance of Pinus taeda wood fungus Trametes versicolor and of Eucalyptus grandis fungus Gloeophyllum trabeum . In general it is concluded that treatment in an oven resulted in more resistant to biological degradation and lower weight loss compared with the combined treatment of the autoclave with electric oven while the latter when applied to both species resulted in an increase in the propensity of biological degradation.

  8. Aplicação de uma técnica alternativa de manejo físico do solo no cultivo de Eucalyptus grandis W.Hill (Myrtaceae Application of an alternative technique for physical soil management in cultivation of Eucalyptus grandis W.Hill (Myrtaceae

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    João Paulo de Maçaneiro

    2013-02-01

    Full Text Available O objetivo deste estudo foi avaliar o processo de crescimento do Eucalyptus grandis quando submetido à irregularização do terreno. Baseando-se na "Ótica da Teoria do Caos" e partindo-se da hipótese de que as áreas reflorestadas por E. grandis são consideravelmente sensíveis às condições iniciais de preparação do solo, aplicou-se a técnica das rugosidades (variações do relevo alternando superfícies côncavas e convexas para desencadear ao longo do tempo propriedades emergentes que aceleram o processo de crescimento vegetal. A área de estudo localiza-se na Bacia Hidrográfica do Rio Itajaí, em Brusque, SC. Esta foi dividida em quatro parcelas menores: duas com tratamentos irregulares (IR-A e IR-B e outras duas com tratamentos regulares (R-A e R-B. Os tratamentos irregulares consistiram na abertura de cavas, utilizando-se uma retroescavadeira hidráulica, intercaladas com 1 m de largura, 4 a 5 m de comprimento e 0,5 m de profundidade. Nos tratamentos regulares foi adotado o cultivo mínimo do solo, onde o preparo do solo ficou restrito às linhas ou covas de plantio. Na análise do desenvolvimento de E. grandis (altura, diâmetro do colo e na altura do peito - DAP verificou-se diferenças estatísticas entre as técnicas de preparação do solo, sendo os maiores valores nos tratamentos irregulares. Nas parcelas irregulares (IR-A e IR-B foram encontrados os maiores valores médios de altura (5,29 m e 5,46 m, diâmetro do colo (45,65 mm e 45,4 mm e DAP (4,44 cm e 4,79 cm, respectivamente. Pressupõe-se que as rugosidades funcionaram efetivamente como componentes auxiliares na internalização da matéria, retendo água, sedimentos e nutrientes, fato que deve ter potencializado e acelerado o crescimento do E. grandis.The aim of this study was to evaluate the process of growth of Eucalyptus grandis non-regularization when subjected to the ground. Relying on "Optical Chaos Theory" and starting from the assumption that the reforested

  9. Within plant distribution of Anthonomus grandis (Coleoptera: Curculionidae feeding and oviposition damages in cotton cultivars Distribuição vertical de botões florais com danos de alimentação e de oviposição de Anthonomus grandis (Coleoptera: Curculionidae em cultivares de algodoeiro

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    José Fernando Jurca Grigolli

    2013-02-01

    Full Text Available The feeding and oviposition behavior of boll weevil in new cotton cultivars is essential for an adequate management. The objective of this study was to evaluate the vertical distribution of squares punctured for feeding and oviposition of the pest in the cultivars NuOPAL, DeltaOPAL, FMT-701, FMX-910 and FMX-993, and record the most and least preferred times of feeding and oviposition. The number of squares used for boll weevil feeding and oviposition were evaluated weekly in three parts of plant canopy. It was observed that, regardless the cultivar, A. grandis preferred to lay eggs in squares located in the upper part and feed on squares in the middle and upper parts. The boll weevil preferred to feed on cultivar FMT-701 in the beginning of the period of cotton flowering and fruiting, and the cultivars NuOPAL, DeltaOPAL, FMX-910 and FMX-993 throughout the whole period of flowering and fruiting. A. grandis preferred to lay eggs on cultivars NuOPAL, FMT-701 and FMX-993 at the beginning and end of flowering and fruiting of plants, while the cultivars DeltaOPAL and FMX-910 are used for oviposition throughout the period of flowering and fruiting.O conhecimento do comportamento de alimentação e de oviposição de Anthonomus grandis em cultivares recentes de algodoeiro é essencial para seu manejo. Neste trabalho, objetivou-se avaliar a distribuição vertical de botões florais com orifícios de alimentação e de oviposição da praga nas cultivares NuOPAL, DeltaOPAL, FMT-701, FMX-910 e FMX-993, bem como registrar as épocas de maior e menor preferência alimentar e de oviposição. O experimento foi conduzido em Jaboticabal, SP, Brasil, safra 2010/2011. Foram realizadas avaliações semanais, baseadas no número de botões florais, utilizados para alimentação e para oviposição pelo bicudo-do-algodoeiro, em três regiões do dossel das plantas. Observou-se que A. grandis preferiu ovipositar em botões florais localizados no terço superior das

  10. Registro de nido de Camponotus rufipes (Formicidae: Hymenoptera en un armario metálico dentro de una estructura urbana | Nesting report of Camponotus rufipes (Formicidae: Hymenoptera in a metallic cabinet insight an urban structure

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    Cristina Sainz-Borgo

    2017-11-01

    Full Text Available Urban ants are common and have a great importance for humans, for the effects in the houses or for being vectors of pathogens. The present paper reports the presence of a nest of Camponotus atriceps (Formicidae: Hymenoptera inside a metal cabinet in a research laboratory at Simón Bolívar University (Caracas, Venezuela. This report constitutes one of the few records for this species in metallic structures, since they usually occupy wooden structures.

  11. Prey identification in nests of the potter wasp Hypodynerus andeus (Packard (Hymenoptera, Vespidae, Eumeninae using DNA barcodes

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    Héctor A. Vargas

    2014-06-01

    Full Text Available Prey identification in nests of the potter wasp Hypodynerus andeus (Packard (Hymenoptera, Vespidae, Eumeninae using DNA barcodes. Geometrid larvae are the only prey known for larvae of the Neotropical potter wasp Hypodynerus andeus (Packard, 1869 (Hymenoptera, Vespidae, Eumeninae in the coastal valleys of the northern Chilean Atacama Desert. A fragment of the mitochondrial gene cytochrome oxidase c subunit 1 was amplified from geometrid larvae collected from cells of H. andeus in the Azapa Valley, Arica Province, and used to provide taxonomic identifications. Two species, Iridopsis hausmanni Vargas, 2007 and Macaria mirthae Vargas, Parra & Hausmann, 2005 were identified, while three others could be identified only at higher taxonomic levels, because the barcode reference library of geometrid moths is still incomplete for northern Chile.

  12. Seasonality of Pelecinus polyturator (Drury (Hymenoptera, Pelecinidae in the Atlantic Rainforest of São Paulo State, Brazil

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    Rogéria I. R. Lara

    2014-03-01

    Full Text Available Seasonality of Pelecinus polyturator (Drury (Hymenoptera, Pelecinidae in the Atlantic Rainforest of São Paulo State, Brazil. A survey of the parasitoid wasp Pelecinus polyturator (Drury, 1773 (Hymenoptera, Pelecinidae was carried out with five Malaise traps/area in five areas in the Atlantic Rainforest of São Paulo State, Brazil, between November 2009 and October 2010. The sampling effort in each locality amounted to 1,825 trap-days. Data were obtained from a total of 317 exemplars of P. polyturator, corresponding to 108 females and 209 males. The average sex ratio of the studied population was 0.52. The highest occurrence of P. polyturator was observed between November and March with frequency peak in January; about 95% of the specimens studied were captured at altitudes close to 1,000 m above sea level.

  13. A new species of solitary Meteorus (Hymenoptera: Braconidae) reared from caterpillars of toxic butterflies (Lepidoptera: Nymphalidae) in Ecuador.

    Science.gov (United States)

    Shaw, Scott R; Jones, Guinevere Z

    2009-01-01

    A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nymphalidae. A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nymphalidae.

  14. Predation of Apiomerus pilipes (Fabricius (Hemiptera, Reduviidae, Harpactorinae, Apiomerini over Meliponinae bees (Hymenoptera, Apidae, in the State of Amazonas, Brazil

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    Alexandre Coletto da Silva

    2004-12-01

    Full Text Available The present work shows the occurrence of an intense predatory activity on adults working Meliponinae bees (Hymenoptera, Apidae, by Apiomerus pilipes (Fabricius, 1787 (Hemiptera, Reduviidae, Harpactorinae, Apiomerini at a meliponary in the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas State, Brazil.O presente trabalho registra a ocorrência de intensa atividade predatória de Apiomerus pilipes (Fabricius, 1787 (Hemiptera, Reduviidae, Harpactorini, Apiomerini sobre operárias adultas de meliponíneos (Hymenoptera, Apidae, no meliponário experimental do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Estado do Amazonas, Brasil. O meliponário se encontra num fragmento de vegetação secundária no próprio INPA.

  15. Higher-level bee classifications (Hymenoptera, Apoidea, Apidae sensu lato Classificação dos grandes grupos de abelhas (Hymenoptera, Apoidea, Apidae sensu lato

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    Gabriel A. R. Melo

    2005-03-01

    Full Text Available A higher-level classification of bees, in which the entire group is treated as a single family - the Apidae - is advocated here. A total of seven subfamilies, 51 tribes and 27 subtribes are recognized. These subfamilies correspond to the families adopted in the traditional classification. Although the proposed changes do not involve any major rearrangement, basically only changing the rank given to the main groups, the new system makes the classification of bees more consistent with that adopted for other major groups of aculeate Hymenoptera. It also departs from the 19th century practice, perpetuated in the traditional classification, of giving family-status to the main groups of bees. A correspondence table associating the taxon names used in the current traditional classification with those of the revised classification is presented. Scrapterini new tribe (type-genus Scrapter Lepeletier & Serville is proposed to accommodate the southern African genus Scrapter.Apresenta-se uma classificação para as abelhas em que o todo o grupo é tratado como uma única família - Apidae. São reconhecidas sete subfamílias, 51 tribos e 27 subtribos. As subfamílias correspondem às famílias da classificação tradicional. Apesar das mudanças propostas afetarem apenas o status dos grupos, o novo sistema torna a classificação das abelhas mais consistente com aquela adotada para os grandes grupos de Hymenoptera aculeados. Além disso, distancia-se da tradição de dar status de família aos grupos principais de abelhas, uma prática do século 19 perpetuada na classificação tradicional. É apresentada uma tabela de correspondência associando os nomes dos táxons usados na classificação tradicional corrente com aquelas da classificação sendo proposta aqui. Scrapterini tribo nova (gênero-tipo Scrapter Lepeletier & Serville é proposta para acomodar Scrapter, um gênero restrito à porção sul do continente africano.

  16. The discovery of the genus Spasskia Belokobylskij, 1989 (Hymenoptera: Braconidae) in China, with description of a new species.

    Science.gov (United States)

    Yan, Cheng-jin; He, Jun-hua; Chen, Xue-xin

    2014-01-01

    The genus Spasskia Belokobylskij, 1989 (Hymenoptera: Braconidae: Helconinae) is reported for the first time from China. Two species, namely Spasskia brevicarinata Yan et Chen sp. n.and Spasskia indica Singh, Belokobylskij et Chauhan, 2005 are described and illustrated. A key to the species of this genus is updated to include the new species. This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.

  17. Temporal Activity Patterns of the Spider Wasp Pepsis montezuma Smith (Hymenoptera: Pompilidae) in a Disturbed Lower Montane Rainforest (Manizales, Colombia)

    OpenAIRE

    Restrepo-Giraldo, Carlos; Rodriguez, Juanita; Pitts, James P.

    2012-01-01

    We studied the temporal activity pattern of the spider wasp Pepsis montezuma Smith (Hymenoptera: Pompilidae) in a disturbed lower montane rainforest, which is located in the city of Manizales, Colombia, at an altitude of 2,150 m. Females of this species are diurnal with two peaks of activity: one in the morning and the other in the afternoon. During the morning, nectar foraging occurred at Baccharis latifolia. During the afternoon, females hunted for tarantulas of the genus Pamphobeteus (Aran...

  18. Risk of anaphylaxis in patients with large local reactions to hymenoptera stings: a retrospective and prospective study.

    Science.gov (United States)

    Pucci, Stefano; D'Alò, Simona; De Pasquale, Tiziana; Illuminati, Ilenia; Makri, Elena; Incorvaia, Cristoforo

    2015-01-01

    In the few studies available, the risk of developing systemic reactions (SR) to hymenoptera stings in patients with previous large local reactions (LLRs) to stings ranges from 0 to 7 %. We evaluated both retrospectively and prospectively the risk of SRs in patients with LLRs to stings. An overall number of 477 patients, 396 with an SR as the first manifestation of allergy and 81 with a history of only LLRs after hymenoptera stings, were included in the study. All patients had clinical history and allergy testing (skin tests and/or specific IgE) indicative of allergy to venom of only one kind of Hymenoptera. Of the 81 patient with LLRs, 53 were followed-up for 3 years by annual control visits, while the 396 patients with SR were evaluated retrospectively. Among the 396 patients with an SR, only 17 (4.2 %) had had a previous LLR as debut of allergy, after an history of normal local reactions to Hymenoptera stings. All the 81 patients with a history of only LLRs had previously had at least two LLRs, with an overall number of 238 stings and no SR. Among the 53 patients who were prospectively evaluated we found that 31 of them (58.3 %) were restung by the same type of insect, with an overall number of 59 stings, presenting only LLRs and no SR. Our findings confirm that patients with repeated LLRs to stings had no risk of SR, while a single LLR does not exclude such risk. This has to be considered in the management of patients with LLRs.

  19. Impact of Hymenoptera venom allergy and the effects of specific venom immunotherapy on mast cell metabolites in sensitized children

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    Ewa Cichocka-Jarosz

    2014-06-01

    Full Text Available introduction and objective. Mast cells (MC are effector cells during severe systemic reactions (SR to Hymenoptera stings. Venom specific immunotherapy (VIT is the treatment of choice for prevention of SR to stings. Tryptase and prostaglandin D[sub]2[/sub] metabolites (PGD[sub]2[/sub] are the markers of MC activation. The study design was to 1. compare baseline values of serum tryptase concentration (BST and PGD[sub]2[/sub] metabolites in children with/without venom sensitization, 2. to evaluate an influence of rush VIT on MC markers in treated children. materials and methods. Sensitized group: 25 children with SR to Hymenoptera sting. Control group: 19 healthy children. Active treatment: 5-day-rush-VIT. BST was evaluated by ImmunoCAP, PGD[sub]2[/sub] metabolites in blood and urine by GC-NICI-MS. results. The baseline blood levels of MC markers were significantly higher, while urinary concentration of 9α,11β-PGF2 was significantly lower in the whole group of venom-sensitized children compared to controls. Severity of SR showed negative correlation with urinary PGD[sub]2[/sub] metabolites, while positive with plasma 9α,11β-PGF2 and BST concentration The highest sensitivity was obtained for plasma 9α,11β-PGF2 whereas the highest specificity for urinary PGD-M. conclusions. In children with IgE-mediated SR to Hymenoptera stings, elevation of baseline values of PGD2 metabolites in blood is accompanied by decreased excretion of its urinary metabolites. Assessment of stable PGD[sub]2 [/sub] metabolites might serve as an independent MC marker to identify allergic children. There is an association between urinary PGD[sub]2[/sub] metabolites and severity of the SR to Hymenoptera stings.

  20. A New Species of Solitary Meteorus (Hymenoptera: Braconidae) Reared from Caterpillars of Toxic Butterflies (Lepidoptera: Nymphalidae) in Ecuador

    OpenAIRE

    Shaw, Scott R.; Jones, Guinevere Z.

    2009-01-01

    A new species of parasitoid wasp, Meteorus rugonasus Shaw and Jones (Hymenoptera: Braconidae), is described from the Yanayacu Biological Station, Napo Province, Ecuador. The new species is diagnosed and compared to other species in the genus. It was reared from larvae of Pteronymia zerlina (Hewitson, 1855) (Lepidoptera: Nymphalidae, Ithomiinae) found feeding on leaves of Solanum (Solanaceae). The parasitoid is solitary. This is the first record of a Meteorus species attacking ithomiine Nympha...

  1. Evaluation of the quality of life in subjects with a history of severe anaphylactic reaction to the Hymenoptera venom.

    Science.gov (United States)

    Nowak, Natalia; Bazan-Socha, Stanisława; Pulka, Grażyna; Pełka, Karolina; Latra, Paulina

    2015-01-01

    Sensitization to the Hymenoptera venom is one of the main causes of anaphylaxis in Poland. Venom immunotherapy is the only effective treatment in such cases. Comprehensive patient care includes also education. The aim of our study was to assess the state of knowledge and to evaluate the quality of life and the anxiety level in patients allergic to the Hymenoptera venom after anaphylactic reaction. The survey was carried out in the period of the insects flight in 61 adult subjects (35 wasp and 26 bee allergic), using a validated Vespid Allergy Quality of Life Questionnaire (VQLQ), Hospital Anxiety and Depression Scale, and subjective assessment of anxiety level. The majority of respondents received venom immunotherapy. Sensitized to the wasp venom had significantly impaired quality of life (VQLQ score) as compared to the bee venom allergic (p = 0.014). The intensity of anxiety decreased with the duration of immunotherapy (p = 0.01). The majority of subjects knew how to recognize and treat anaphylaxis, but only 8% employed an identification card and about 50% implemented rules of the pre-exposition prophylaxis. History of a severe anaphylaxis to the Hymenoptera venom affected the quality of life. Venom immunotherapy reduced anxiety. We hope that presented surveys and their results might be useful in qualifying for immunotherapy in clinically uncertain cases.

  2. Produção de biomassa e remoção de nutrientes em povoamentos de Eucalyptus camaldulensis Dehnh, Eucalyptus grandis hill ex Maiden e Eucalyptus torelliana F. Muell, plantados em Anhembí, SP.

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    Mauro Valdir Schumacher

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Neste trabalho estudou-se a distribuição de biomassa e a quantidade de nutrientes estocados nos diferentes compartimentos (folhas, ramos, casca e lenho das árvores de Eucalyptus camaldulensis Dehnh, Eucalyptus grandis Hill ex Maiden e Eucalyptus torelliana F. Muell com 9, 9 e 12 anos de idade, respectivamente, plantados em solos de textura arenosa e baixa fertilidade, em Anhembi - SP. A espécie E. grandis foi a que apresentou a maior produção de biomassa para todos os compartimentos analisados, com exceção dos ramos grossos. Nas três espécies o tronco (casca + lenho representou em média 90% da biomassa acima do solo. Os nutrientes concentraram-se de forma decrescente nas folhas, ramos, casca e lenho. As copas das árvores foram responsáveis pelo acúmulo de, aproximadamente, 24% dos nutrientes contidos na biomassa total das árvores. Na casca encontram-se as maiores quantidades de cálcio, aproximadamente 60% do total. A espécie E. grandis removeu do solo a maior quantidade de nutrientes, desenvolvendo através da queda de folhedo menores quantidades que as outras espécies. Desta forma cuidados especiais deverão ser dispensados para garantir a produtividade das rotações futuras.

  3. Ten unique and charismatic new species of Microgastrinae wasps (Hymenoptera, Braconidae from North America

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    Jose Fernandez-Triana

    2018-01-01

    Full Text Available Ten new species within four genera of Microgastrinae parasitoid wasps (Hymenoptera: Braconidae are described from Canada and United States: Diolcogaster ichiroi, Diolcogaster miamensis, Glyptapanteles pseudotsugae, Microgaster archboldensis, Microgaster syntopic, Microplitis altissimus, Microplitis jorgeluisi, Microplitis juanmanueli, Microplitis julioalbertoi, and Microplitis mariamargaritae. The new taxa are significant because they represent the first North American records of a tropical group (species of the basimacula group in Diolcogaster, exemplify interesting ecological cases (niche-based host selection in Glyptapanteles, syntopic species in Microgaster, and showcase unique morphological features and/or altitudinal records (Microplitis. Most of the new species were collected in protected areas or areas with strong research programs (Archbold Biological Station and hammock forests near Miami, Florida; Great Sand Dunes National Park and Preserve, and Mount Evans Wilderness Area, Colorado; Sapelo Island, Georgia; Tonto National Forest, Arizona, and thus are also of value and interest for conservation and research efforts.

  4. Description of five species of Xanthopimpla Saussure 1892 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia

    Science.gov (United States)

    Dass, Angeline David; Ghani, Idris Abd.

    2013-11-01

    Description of five species of Xanthopimpla Saussure, 1829 (Hymenoptera: Ichneumonidae: Pimplinae) from Malaysia was done using specimens deposited in Centre for Insects Systematics, Universiti Kebangsaan Malaysia (CIS, UKM). Type and non-type specimens were loaned from several repositories namely Zoological Museum of Amsterdam Netherlands (ZMAN), Swedish Museum of Natural History (NRM), British Natural History Museum London (BMNH) and Department of Agricultural Malaysia (DOA) for identification and comparison. The specimens were identified to the species level which gives rise to five species namely Xanthopimpla conica Cushman, 1925, Xanthopimpla despinosa leipephelis Townes & Chiu, 1970, Xanthopimpla flavolineata Cameron, 1907, Xanthopimpla punctata (Fabricius, 1781) and Xanthopimpla tricapus impressa Townes & Chiu, 1970. A dichotomous key and descriptions for five Xanthopimpla spesies were provided. Photos and illustrations of carina on propodeum were also included in this paper.

  5. Antibacterial Compounds from Propolis of Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae) from Thailand

    Science.gov (United States)

    Sanpa, Sirikarn; Popova, Milena; Bankova, Vassya; Tunkasiri, Tawee; Eitssayeam, Sukum; Chantawannakul, Panuwan

    2015-01-01

    This study investigated the chemical composition and antimicrobial activity of propolis collected from two stingless bee species Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae). Six xanthones, one triterpene and one lignane were isolated from Tetragonula laeviceps propolis. Triterpenes were the main constituents in T. melanoleuca propolis. The ethanol extract and isolated compounds from T. laeviceps propolis showed a higher antibacterial activity than those of T. melanoleuca propolis as the constituent α-mangostin exhibited the strongest activity. Xanthones were found in propolis for the first time; Garcinia mangostana (Mangosteen) was the most probable plant source. In addition, this is the first report on the chemical composition and bioactivity of propolis from T. melanoleuca. PMID:25992582

  6. Morphometry of the midgut of Melipona quadrifasciata anthidioides (Lepeletier) (Hymenoptera: Apidae) during metamorphosis.

    Science.gov (United States)

    Cruz, L C; Araújo, V A; Dolder, H; Araújo, A P A; Serrão, J E; Neves, C A

    2011-01-01

    In Hymenoptera, midgut changes begin in the last instar. At this stage, the larval epithelial digestive cells degenerate, leaving only the basal membrane and the regenerative cells which will develop into a new epithelium during the pupal stage and in the adult. Epithelium renewal is followed by changes in volume and shape of the midgut. Morphometric analysis of digestive cells and total midgut volume of Melipona quadrifasciata anthidioides (Lepeletier) were conducted to verify whether cell volume increase are sufficient to account for the total midgut volume increase that occurs during metamorphosis. An increase in midgut volume was verified in spite of the scarcity of cell proliferation found during metamorphosis. At the end of metamorphosis, the increase in cell volume was not sufficient to explain the increase in volume of the midgut, indicating that an increase in the number of digestive cells is apparently necessary. Nevertheless, the mechanism by which regenerative cells reconstitute the epithelium during metamorphosis remains unknown.

  7. Nesting sites characteristics of stingless bees (Hymenoptera: Apidae in Central Sulawesi, Indonesia

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    Nelky Suriawanto

    2017-06-01

    Full Text Available Stingless bees (Hymenoptera: Apidae is eusocial insects that live together in a colony. This research was aimed to study the nesting site characteristics of stingless bees in the settlement areas at Central Sulawesi, Indonesia. The nesting sites were observed by purposive sampling method from July 2015 to January 2016. Four species belong to genus Tetragonula were found, namely T. fuscobalteata, T. biroi, T. sapiens, and T. laeviceps. Two spesies, T. biroi and T. sapiens are the new record in Sulawesi island. The highest abundance of stingless bees colony was T. fuscobalteata (92.26%, followed by T. biroi (4.17%, T. sapiens (2.98%, and T. laeviceps (0.59%. Nesting sites of T. fuscobalteata were found in the stone, brick wall, wooden wall, bamboo, and iron cavities, T. biroi in the wooden wall, stone, and brick wall cavities, T. sapiens in stone cavities, while T. laeviceps in wooden walls.

  8. A new mesoserphid wasp from the Middle Jurassic of northeastern China (Hymenoptera, Proctotrupoidea

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    Yan Zheng

    2017-12-01

    Full Text Available A new genus and species of Mesoserphidae (Hymenoptera, Juraserphus modicus gen. et sp. nov., is described based on a well-preserved fossil specimen from the Middle Jurassic Jiulongshan Formation of northeastern China. It is characterized by the following forewing features: the forking of Rs+M located approximately one-third of the distance between 1m-cu and 2r-rs, both 1cu-a and 2cu-a antefurcal; 1-M more than twice as long as 1m-cu and hind wing with cells r and rm closed. In addition, it has a short ovipositor, only extending slightly beyond the metasomal apex. Its new morphological characters broaden the diversity of Mesoserphidae in the Mesozoic and provide new insights into the evolution and relationships of Mesoserphidae.

  9. Additions to the Knowledge of the Genus Phimenes (Hymenoptera: Vespidae: Eumeninae from Vietnam

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    Lien Thi Phuong

    2016-01-01

    Full Text Available The solitary wasp genus Phimenes Giordani Soika, 1992 (Hymenoptera: Vespidae: Eumeninae was reported to occur in Vietnam by van der Vecht (1959, represented by one taxon, Phimenes flavopictus continentalis (Zimmermann, which was synonymized under nominotypical Phimenes flavopictus (Blanchard, 1849 by Kumar (2013. A note on gender of this genus is made in the text. One more species, Phimenes indosinensis (van der Vecht, 1959 is recorded in this study from Dak Lak in the southern and Son La in the northwestern parts of Vietnam for the first time. Detailed descriptions of the female and male of the latter are provided with figures. A key to the two species from Vietnam is also provided.

  10. Insecticide toxicity to Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) females and effect on descendant generation.

    Science.gov (United States)

    Vianna, Ulysses R; Pratissoli, Dirceu; Zanuncio, José C; Lima, Eraldo R; Brunner, Jay; Pereira, Fabrício F; Serrão, José E

    2009-02-01

    The effect of nine insecticides used in tomato production was evaluated on adults of two Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) populations from Rive and Afonso Cláudio, Espírito Santo State, Brazil. The experiment was developed in an acclimatized chamber at 25 +/- 1 degrees C, 70 +/- 10% relative humidity and 14 h photophase. Eggs of Anagasta kuehniella (Lepidoptera: Pyralidae), previously immersed in insecticides solutions were offered to females of both T. pretiosum populations. Bacillus thuringiensis, lufenuron and triflumuron had lowest negative effects on parasitism and viability of individuals of these populations; however, abamectin and pyrethroids (betacyflurin 50 and 125 g/l and esfenvalerate) insecticides reduced parasitism rates. T. pretiosum emerged from A. kuehniella eggs treated with esfenvalerate but were not able to parasitize non treated eggs of this host. B. thuringiensis, lufenuron and triflumuron may be used in integrated pest management programs to control tomato pests, because they have moderated negative effect on parasitoid wasps.

  11. Revision of the Palaearctic Gasteruption assectator aggregate, with special reference to Sweden (Hymenoptera, Gasteruptiidae

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    Niklas Johansson

    2016-09-01

    Full Text Available The Palaearctic species of the Gasteruption assectator aggregate (Hymenoptera, Gasteruptiidae are revised and three species are recognised. Two species are re-instated: Gasteruption boreale (Thomson, 1883, stat. n. and G. nigritarse (Thomson, 1883, stat. n., and both are excluded from the synonymy with G. assectator (Linnaeus, 1758. The general distribution of both species is given for Europe and in detail for Sweden. A key to the valid Palaearctic species of the Gasteruption assectator aggregate is given; key characters and primary types are illustrated. Four new synonyms are listed: Foenus fumipennis Thomson, 1883, Trichofoenus breviterebrae Watanabe, 1934, and Gasteruption margotae Madl, 1987, are synonymized with Gasteruption boreale (Thomson, 1883 and Gasteruption brevicauda Kieffer, 1904, with G. undulatum (Abeille de Perrin, 1879.

  12. Antibacterial Compounds from Propolis of Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae from Thailand.

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    Sirikarn Sanpa

    Full Text Available This study investigated the chemical composition and antimicrobial activity of propolis collected from two stingless bee species Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae. Six xanthones, one triterpene and one lignane were isolated from Tetragonula laeviceps propolis. Triterpenes were the main constituents in T. melanoleuca propolis. The ethanol extract and isolated compounds from T. laeviceps propolis showed a higher antibacterial activity than those of T. melanoleuca propolis as the constituent α-mangostin exhibited the strongest activity. Xanthones were found in propolis for the first time; Garcinia mangostana (Mangosteen was the most probable plant source. In addition, this is the first report on the chemical composition and bioactivity of propolis from T. melanoleuca.

  13. Development of antennal sensilla of Tetragonisca angustula Latreille, 1811 (Hymenoptera: Meliponini during pupation

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    V. T. Dohanik

    Full Text Available Abstract The antennal sensilla are sensory organs formed by a group of neurons and accessory cells, which allow perception of environmental cues, which play a role as mechanoreceptors and chemoreceptors. This study describes the post-embryonic development of the antennal sensilla of the stingless Tetragonisca angustula (Hymenoptera: Meliponini workers. The development of the antennal sensilla begins in the transition stage of the pre-pupae to white-eyed pupae. The sensilla are completely developed at the black-eyed pupae stage, but they are covered by the old cuticle. The sensilla are exposed to the environment only in newly emerged workers of T. angustula, but it is possible that environmental stimuli can be recognized due to the pores in the old cuticle.

  14. Lymphocyte-mediated regulation of platelet activation during desensitization in patients with hymenoptera venom hypersensitivity.

    Science.gov (United States)

    Ledru, E; Pestel, J; Tsicopoulos, A; Joseph, M; Wallaert, B; Tonnel, A B; Capron, A

    1988-01-01

    T cells from peripheral blood of hymenoptera sensitive patients were studied before and after venom desensitization. Before treatment, T cells showed a variable but higher proliferative response to allergen than T cells of treated patients or controls. While before desensitization, T cell products, specifically released after in vitro allergen stimulation, were able to amplify the IgE-dependent platelet activity, we showed that after treatment of the same patients, T cell products strongly reduced platelet activation. Considering the modifications in platelet activation previously observed in patients treated by specific immunotherapy, the present results suggest that, through a modification of T cell reactivity to allergen, T cell functions are modulated by desensitization, and emphasize the involvement of T cell products in the desensitization mechanisms. PMID:3263227

  15. Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae)

    International Nuclear Information System (INIS)

    Pratissoli, Dirceu; Oliveira, Harley N. de; Oliveira, Regiane C. de; Zago, Hugo B.; Vieira, Stella M.J.

    2004-01-01

    Effect of host and food availability on the biological characteristics of Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae). Biological characteristics of Trichogramma galloi Zucchi, 1988 were evaluated in laboratory where these parasitoids were reared on eggs of Diatraea saccharalis (Lepidoptera, Pyralidae) with or without honey, and exposed to eggs of the host after 0, 6, 12, 24, 36, 48, 60, 72 and 84 hours of emergence. The parasitism rate and viability showed higher for individuals that received food. The sex ratio was not influenced by food. The number of individuals per egg only showed difference for those adults that did not receive food and stayed six hours without the host eggs. Checking the effect of the availability of eggs, only the sex ratio, with or without honey, did not show differences. The results show that T. galloi needs a carbohydrate supply and the time can influence the reproductive capacity. (author)

  16. First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae

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    Thiago S. Montagna

    2012-06-01

    Full Text Available First report of interspecific facultative social parasitism in the paper wasp genus Mischocyttarus Saussure (Hymenoptera, Vespidae. Parasitism of colonies of the social wasp Mischocyttarus cerberus Ducke, 1918 by females of Mischocyttarus consimilis Zikán, 1949 was observed in a rural area of Dourados, state of Mato Grosso do Sul, Brazil. In all monitored cases, the invasion occurred in the pre-emergence colony stage, generally by a single female of M. consimilis. The period of establishment of the foreign female in the host colony was marked by antagonistic behaviors between the host female and the invasive. In general, the architecture of the parasitized nest was modified from the typical architecture of the host species nest.

  17. Ophioninae (Hymenoptera: Ichneumonidae wasp community in the cloudy forest Monteseco, Cajamarca, Peru

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    Evelyn Sánchez

    2014-12-01

    Full Text Available We describe the species composition of the subfamily Ophioninae (Hymenoptera: Ichneumonidae along an altitudinal gradient in the cloudy forest Monteseco, Cajamarca, Peru collected in 2009 and 2010. Eighteen species were recorded in three genera of Ophioninae: Alophophion, Enicospilus y Ophion. Five species are recorded for the first time in Peru: Ophion polyhymniae Gauld, 1988; Enicospilus cubensis (Norton, 1863; E. guatemalensis (Cameron, 1886; E. cressoni Hooker, 1912 y E. mexicanus (Cresson, 1874. Subfamily composition varies with the elevation. The highest species richness (S=11 was found at 2150 m and the lowest (S=3 at 3116 m. Enicospilus is more diverse from low to mid elevation, Ophion from mid to high elevation and Alophophion occurs predominantly at high elevation.

  18. Notes on the systematics of the orchid-bee genus Eulaema (Hymenoptera, Apidae

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    Gabriel A. R. Melo

    2014-09-01

    Full Text Available Notes on the systematics of the orchid-bee genus Eulaema (Hymenoptera, Apidae. The classification of the genus Eulaema is modified in order to make it congruent with recent phylogenetic hypotheses based on molecular data. The speciosa group, containing E. peruviana, E. speciosa and related species, is removed from E. (Eulaema and transferred to E. (Apeulaema. New morphological characters are presented to support the revised scope of the subgenera and their diagnostic features are revised. Six species groups are recognized herein: two in E. (Apeulaema and four in E. (Eulaema. A list of valid species in each species group and an identification key to males of each of the subgenera and species groups are provided. Finally, an older overlooked designation of a type species for Eulaema is presented in the Appendix.

  19. Dirhinus giffardii (Hymenoptera: Chalcididae, parasitoid affecting Black Soldier Fly production systems in West Africa

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    Emilie Devic

    2015-12-01

    Full Text Available Interest for insect farming is currently growing globally. Conditions in West Africa appear suitable for developing such farming systems that can benefit communities by improving livelihoods, food and feed security or sanitation. In Ghana and Mali, the Black Soldier Fly (Hermetia illucens Linnaeus, 1758 is being produced for waste recycling and animal feed. In a two stages process (egg and larvae production, egg production was hampered by a pupal parasitoid, Dirhinus giffardii Silvestri, 1913 (Hymenoptera: Chalcididae, which reduced future broodstock by almost 72%. This is the first time D. giffardii is reported as a parasitoid of H. illucens pupae and one of the first reports of parasitism in this commercially important fly species. The introduction of precautionary measures is highly recommended for the success of H. illucens production systems in West Africa.

  20. Epidemiology of allergic reactions to hymenoptera stings in Irish school children.

    LENUS (Irish Health Repository)

    Jennings, Aisling

    2010-12-01

    The aim of this was to study generate the first epidemiological data regarding the prevalence of hymenoptera allergy among school children in Ireland. Questionnaires, including six sting-specific questions (1), were distributed to the parents of primary school children aged 6-8 and 11-13, divided equally between rural and urban backgrounds. From 110 schools, 4112 questionnaires were returned. A total of 1544 (37.5%) children had been stung in their lifetime. Among the total, 5.8% of children stung experienced a large local reaction, 3.4% had a mild (cutaneous) systemic reaction (MSR) and 0.8% experienced a moderate\\/severe systemic reaction (SSR); these figures respectively represent 2.2%, 1.3% and 0.2% of the total study group. On logistic regression analysis, older children and rural children were at a higher risk of being stung (OR 1.7; 95% CI 1.4-2.; OR 1.6; 95% CI 1.4-1.8 respectively). Rural dwellers and asthma sufferers were more likely to experience an SSR (OR 4.3; 95% CI 1.4-13.5 and OR 2.8; 95% CI 1.8-4.3, respectively). Hymenoptera stings are more common in rural than urban dwelling Irish children. Asthma imparted a greater risk of SSR in this study population. Severe reactions are unusual overall, occurring in <1% of those stung, a lower prevalence than in Israeli teenagers but in keeping with other European reports relating to young children.