WorldWideScience

Sample records for glycolytic network restructuring

  1. Plasticity-induced restructuring of a nanocrystalline grain boundary network

    International Nuclear Information System (INIS)

    Panzarino, Jason F.; Pan, Zhiliang; Rupert, Timothy J.

    2016-01-01

    The grain boundary-mediated mechanisms that control plastic deformation of nanocrystalline metals should cause evolution of the grain boundary network, since they directly alter misorientation relationships between crystals. Unfortunately, current experimental techniques are unable to track such evolution, due to limits on both spatial and temporal resolution. In this work, molecular dynamics simulations are used to study grain boundary restructuring in nanocrystalline Al during both monotonic tension and cyclic loading. This task is enabled by the creation of new analysis tools for atomistic datasets that allow for a complete characterization and tracking of microstructural descriptors of the grain boundary network. Quantitative measurements of grain boundary character distribution, triple junction type, grain boundary plane normal, and other interfacial network characteristics are extracted and analyzed. The results presented here show that nanocrystalline plasticity leads to an increase in special boundary fraction and disruption of two-dimensional boundary connectivity, with the most dramatic evolution occurring in the smallest grain sizes.

  2. Capillary network in slow and fast muscles and in oxidative and glycolytic muscle fibres

    Czech Academy of Sciences Publication Activity Database

    Čebašek, V.; Kubínová, Lucie; Ribarič, S.; Eržen, I.

    2005-01-01

    Roč. 24, March (2005), s. 51-58 ISSN 1580-3139 Grant - others:SI-CZ(CZ) KONTAKT 19/2005 Institutional research plan: CEZ:AV0Z50110509 Keywords : capillaries * skeletal muscle fibre s-oxidative and glycolytic * stereology Subject RIV: EA - Cell Biology

  3. neural network based load frequency control for restructuring power

    African Journals Online (AJOL)

    2012-03-01

    Mar 1, 2012 ... Abstract. In this study, an artificial neural network (ANN) application of load frequency control. (LFC) of a Multi-Area power system by using a neural network controller is presented. The comparison between a conventional Proportional Integral (PI) controller and the proposed artificial neural networks ...

  4. Neural Network Based Load Frequency Control for Restructuring ...

    African Journals Online (AJOL)

    The comparison between a conventional Proportional Integral (PI) controller and the proposed artificial neural networks controller is showed that the proposed controller can generate an improved ... The same technique is then applied to control a system compose of two single units tied together though a power line.

  5. Electric restructuring and consumer choice: lessons from other network industries

    International Nuclear Information System (INIS)

    Crandall, R. W.

    1999-01-01

    The advantages of the U.S. model of private markets with limited regulation as the best alternative for delivering goods and services to consumers are discussed by citing examples from deregulated industries such as transportation, primary energy and financial markets. In all these cases deregulation has been extraordinarily successful. Experiences from these industries are examined in an effort to extract lessons that might be useful in predicting the likely evolution of competition in the electricity and telecommunications industries. A warning is sounded that deregulating these industries without opening access to the infrastructure (which is owned by carriers) could create major problems of natural-monopoly exploitation by the incumbents that would negate any productive and allocative efficiency gains conferred by deregulation. One obvious choice for liberalizing a network industry with natural-monopoly infrastructure is simply to separate the infrastructure from the delivery of the service as was done with railroads in the United Kingdom. A similar, but less far-reaching example might be the solution devised for natural gas pipelines in the U.S. where pipeline owners opened their infrastructure to competitors, albeit at regulated rates. In the electricity industry, separating power generation from transmission and distribution appears to be fairly simple, provided access to transmission and distribution network is granted. In the telecommunication industry where there is no generation, the natural monopoly may be in the local distribution of traffic to subscribers, hence separation of local distribution from national or regional distribution is the normal way to open up the market to new service providers. Experiences in the U. S., the U. K., Canada and New Zealand in electricity and telecommunications industry deregulation are examined and various pitfalls in current approaches are pointed out. It is the author's contention that announcing a date for the end

  6. Reliability assessment of restructured power systems using reliability network equivalent and pseudo-sequential simulation techniques

    International Nuclear Information System (INIS)

    Ding, Yi; Wang, Peng; Goel, Lalit; Billinton, Roy; Karki, Rajesh

    2007-01-01

    This paper presents a technique to evaluate reliability of a restructured power system with a bilateral market. The proposed technique is based on the combination of the reliability network equivalent and pseudo-sequential simulation approaches. The reliability network equivalent techniques have been implemented in the Monte Carlo simulation procedure to reduce the computational burden of the analysis. Pseudo-sequential simulation has been used to increase the computational efficiency of the non-sequential simulation method and to model the chronological aspects of market trading and system operation. Multi-state Markov models for generation and transmission systems are proposed and implemented in the simulation. A new load shedding scheme is proposed during generation inadequacy and network congestion to minimize the load curtailment. The IEEE reliability test system (RTS) is used to illustrate the technique. (author)

  7. Day-ahead price forecasting in restructured power systems using artificial neural networks

    International Nuclear Information System (INIS)

    Vahidinasab, V.; Jadid, S.; Kazemi, A.

    2008-01-01

    Over the past 15 years most electricity supply companies around the world have been restructured from monopoly utilities to deregulated competitive electricity markets. Market participants in the restructured electricity markets find short-term electricity price forecasting (STPF) crucial in formulating their risk management strategies. They need to know future electricity prices as their profitability depends on them. This research project classifies and compares different techniques of electricity price forecasting in the literature and selects artificial neural networks (ANN) as a suitable method for price forecasting. To perform this task, market knowledge should be used to optimize the selection of input data for an electricity price forecasting tool. Then sensitivity analysis is used in this research to aid in the selection of the optimum inputs of the ANN and fuzzy c-mean (FCM) algorithm is used for daily load pattern clustering. Finally, ANN with a modified Levenberg-Marquardt (LM) learning algorithm are implemented for forecasting prices in Pennsylvania-New Jersey-Maryland (PJM) market. The forecasting results were compared with the previous works and showed that the results are reasonable and accurate. (author)

  8. Adaptive short-term electricity price forecasting using artificial neural networks in the restructured power markets

    International Nuclear Information System (INIS)

    Yamin, H.Y.; Shahidehpour, S.M.; Li, Z.

    2004-01-01

    This paper proposes a comprehensive model for the adaptive short-term electricity price forecasting using Artificial Neural Networks (ANN) in the restructured power markets. The model consists: price simulation, price forecasting, and performance analysis. The factors impacting the electricity price forecasting, including time factors, load factors, reserve factors, and historical price factor are discussed. We adopted ANN and proposed a new definition for the MAPE using the median to study the relationship between these factors and market price as well as the performance of the electricity price forecasting. The reserve factors are included to enhance the performance of the forecasting process. The proposed model handles the price spikes more efficiently due to considering the median instead of the average. The IEEE 118-bus system and California practical system are used to demonstrate the superiority of the proposed model. (author)

  9. What drives Senegalese migration to Europe? The role of economic restructuring, labor demand, and the multiplier effect of networks

    Directory of Open Access Journals (Sweden)

    Pau Baizán

    2016-08-01

    Full Text Available Background: International migration from Sub-Saharan Africa to Europe is poorly understood. Furthermore, existing studies pay insufficient attention to the links between the micro-level factors and political, social, and economic processes in both origin and destination areas. Here we integrate insights from institutional approaches in migration and development research with perspectives that highlight the role of labor market and social capital. Objective: We analyze the contextual and individual-level determinants of migration from Senegal to France, Italy, and Spain since the mid-1970s. We examine the following hypotheses: (1 In Senegal, the deterioration of living conditions and heightened economic insecurity have created the conditions for increasing out-migration propensities. (2 In Europe, labor market restructuring has increased job opportunities in particular places and job niches. (3 In facilitating access of Senegalese migrants to jobs in Europe, social networks have linked these two processes. (4 The conjunction of periods of strong labor demand and the availability of personal networks in Europe creates a boosting effect on the migration probabilities of the Senegalese to Europe. Methods: We use event history models to analyze life course data from the Migrations between Africa and Europe survey (2008. Results: Our empirical results concerning both individual socioeconomic indicators and contextual indicators provide consistent support for the four hypotheses proposed. Conclusions: The initiation and expansion of migration between Senegal and Europe stem from the simultaneous presence of several key factors at origin and destination, including processes of economic restructuring and the mutually reinforcing process of social capital formation and changing labor market conditions. These factors are historically contingent, but they may have a wider relevance in the explanation of migration from developing countries to developed

  10. Cost restructuring

    International Nuclear Information System (INIS)

    Schmidt, J.A.

    1991-01-01

    This paper reports on the cost restructuring of the petroleum industry. This current decade is likely to be one of the most challenging for the petroleum industry. Though petroleum remains among the world's biggest businesses, news of consolidations, restructuring, and layoffs permeates the oil patch from the Gulf of Mexico to the Arctic Isles. The recessionary economy has accelerated these changes, particularly in the upstream sector. Today, even the best-managed companies are transforming their cost structures, and companies that fail to do likewise probably won't survive as independent companies. Indeed, significant consolidation took place during the 1980s. More consolidations can be expected in this decade for companies that do not adapt to the economic realities of the mature business

  11. Restructuring the crystalline cellulose hydrogen bond network enhances its depolymerization rate

    Science.gov (United States)

    Shishir P.S. Chundawat; Giovanni Bellesia; Nirmal Uppugundla; Leonardo da Costa Sousa; Dahai Gao; Albert M. Cheh; Umesh P. Agarwal; Christopher M. Bianchetti; George N. Phillips; Paul Langan; Venkatesh Balan; S. Gnanakaran; Bruce E. Dale

    2011-01-01

    Conversion of lignocellulose to biofuels is partly inefficient due to the deleterious impact of cellulose crystallinity on enzymatic saccharification. We demonstrate how the synergistic activity of cellulases was enhanced by altering the hydrogen bond network within crystalline cellulose fibrils. We provide a molecular-scale explanation of these phenomena through...

  12. Management restructuring: toward a leaner organization.

    Science.gov (United States)

    Doerge, J; Hagenow, N

    1995-12-01

    The drive to create integrated delivery networks has given even further momentum to management restructuring. Through centralization of decision making within networks, speed and flexibility are needed to make the difficult modifications required to achieve standardization and general overhead savings. Two hospitals, Carondelet St. Mary's and Carondelet St. Joseph's merged under a single administrative team and one Vice President of Patient Care Services. Outcomes of this restructuring were monetary savings, but also learning how to plan and carry out major management restructuring. The values, the process and the outcomes one year post implementation, and the keys to success in the management restructuring effort are the subjects of this article.

  13. Operational reliability evaluation of restructured power systems with wind power penetration utilizing reliability network equivalent and time-sequential simulation approaches

    DEFF Research Database (Denmark)

    Ding, Yi; Cheng, Lin; Zhang, Yonghong

    2014-01-01

    In the last two decades, the wind power generation has been rapidly and widely developed in many regions and countries for tackling the problems of environmental pollution and sustainability of energy supply. However, the high share of intermittent and fluctuating wind power production has also...... and reserve provides, fast reserve providers and transmission network in restructured power systems. A contingency management schema for real time operation considering its coupling with the day-ahead market is proposed. The time-sequential Monte Carlo simulation is used to model the chronological...

  14. Restructuring for the 90's...And Beyond. The Era of Smart Homes, Wired Communities, Fast Systems, Global Networks, and Fast Forward Learners in a Borderless World.

    Science.gov (United States)

    Groff, Warren H.

    This paper examines institutional restructuring in higher education for the 1990s and the 21st century and the leadership required for that restructuring. Following an overview and a discussion of selected pertinent demographic, social, economic, technological, and political variables, the paper focuses on the strategic importance of Mid-America…

  15. Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements.

    Directory of Open Access Journals (Sweden)

    Shona A Mookerjee

    Full Text Available Measurements of glycolytic rate and maximum glycolytic capacity using extracellular flux analysis can give crucial information about cell status and phenotype during normal operation, development of pathology, differentiation, and malignant transformation. They are also of great use when assessing the effects of chemical or drug treatments. Here, we experimentally define maximum glycolytic capacity, demonstrate how it differs from glycolytic rate, and provide a protocol for determining the basal glycolytic rate and maximum glycolytic capacity in cells using extracellular flux measurements. The results illustrate the power of extracellular flux analysis to describe the energetics of adherent cells in culture in a fully quantitative way.

  16. Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements.

    Science.gov (United States)

    Mookerjee, Shona A; Nicholls, David G; Brand, Martin D

    2016-01-01

    Measurements of glycolytic rate and maximum glycolytic capacity using extracellular flux analysis can give crucial information about cell status and phenotype during normal operation, development of pathology, differentiation, and malignant transformation. They are also of great use when assessing the effects of chemical or drug treatments. Here, we experimentally define maximum glycolytic capacity, demonstrate how it differs from glycolytic rate, and provide a protocol for determining the basal glycolytic rate and maximum glycolytic capacity in cells using extracellular flux measurements. The results illustrate the power of extracellular flux analysis to describe the energetics of adherent cells in culture in a fully quantitative way.

  17. Modeling diauxic glycolytic oscillations in yeast

    DEFF Research Database (Denmark)

    Hald, Bjørn Olav; Sørensen, Preben Graae

    2010-01-01

    Glycolytic oscillations in a stirred suspension of starved yeast cells is an excellent model system for studying the dynamics of metabolic switching in living systems. In an open-flow system the oscillations can be maintained indefinitely at a constant operating point where they can be characteri...

  18. Global restructuring - A place for ecology?

    NARCIS (Netherlands)

    de Bruijn, Theo; Groenewegen, Peter; Grolin, Jesper

    1997-01-01

    This introductory article summarizes the discussions and results of the Fifth Annual Greening of Industry Conference, Global RestructuringÐA Place for Ecology?, held in Heidelberg, Germany, November 25-27, 1997. The Greening of Industry Network is concerned with the transition of industry towards

  19. High resolution measurement of the glycolytic rate

    Directory of Open Access Journals (Sweden)

    Carla X Bittner

    2010-09-01

    Full Text Available The glycolytic rate is sensitive to physiological activity, hormones, stress, aging and malignant transformation. Standard techniques to measure the glycolytic rate are based on radioactive isotopes, are not able to resolve single cells and have poor temporal resolution, limitations that hamper the study of energy metabolism in the brain and other organs. A new method is described in this article, which makes use of a recently-developed FRET glucose nanosensor to measure the rate of glycolysis in single cells with high temporal resolution. Used in cultured astrocytes, the method showed for the first time that glycolysis can be activated within seconds by a combination of glutamate and K+, supporting a role for astrocytes in neurometabolic and neurovascular coupling in the brain. It was also possible to make a direct comparison of metabolism in neurons and astrocytes lying in close proximity, paving the way to a high-resolution characterization of brain energy metabolism. Single-cell glycolytic rates were also measured in fibroblasts, adipocytes, myoblasts and tumor cells, showing higher rates for undifferentiated cells and significant metabolic heterogeneity within cell types. This method should facilitate the investigation of tissue metabolism at the single-cell level and is readily adaptable for high-throughput analysis.

  20. SE debt restructuring plan

    International Nuclear Information System (INIS)

    Janoska, J.

    2003-01-01

    Slovenske elektrarne, a.s. (SE) plans to restructure one's own debts in 2003-2005. Debt restructuring plan is following: 2003: Collection of pre-payment on electricity - 60 million Euro (2.5 billion Slovak crowns), consumer unknown. Own promissory notes in total value of 100 million US$ (3.5 billion Slovak crowns) - in process. Sale of claims worth 2.4 billion Slovak crowns (57.21 million Euro) - negotiations in process. 2003/2004: Restructuring of loans payable IV. quarter 2003 and at the beginning of 2004 in value of 200 million Euro (8.3 billion Slovak crowns). Aim of SE is a new credit payable within 7 years, with instalments payable in the last two to three without any state subsidies. 2005: Loans worth 460 million Euro (189 billion Slovak crowns) will still remain. SE want to negotiate them with banks without state support

  1. RESTRUCTURING OF INDUSTRIAL ENTERPRISE

    Directory of Open Access Journals (Sweden)

    L. I. Podderegina

    2009-01-01

    Full Text Available The paper presents main principles for execution of current and strategic restructuring of national enterprises while using experience of countries with developed market economy. The principles contribute to higher efficiency in internal industrial relations at national industrial enterprises.

  2. Stimulation-induced cerebral glycolytic glucose metabolism

    International Nuclear Information System (INIS)

    Ackermann, R.F.; Lear, J.L.

    1989-01-01

    The authors have developed a method to estimate the relative amounts of cerebral oxidative and glycolytic glucose metabolism with sequentially administered radiolabeled fluorode-oxyglucose (FDG) and 6-glucose (GLU). Cerebral FDG metabolite concentration was found to reflect total glucose metabolism. Cerebral GLU metabolite concentration, however, was found to reflect mainly oxidative metabolism, because of significant fraction of the radiolabel was lost through lactate production and diffusion from the brain with glycolysis. The authors applied the method to normal rats, to seizing rats, and to optically stimulated rats. Normal cerebral glucose metabolism was primarily oxidative, but stimulation caused profound increases in glycolysis in activated brain regions

  3. Perspectives on industry restructuring

    International Nuclear Information System (INIS)

    Konow, H. R.

    1996-01-01

    An industry association perspective on restructuring in the electrical industry was offered, placing the ongoing debate about deregulation and competition in the industry in Ontario into a Canadian and a global context. It was found that the move towards deregulation was a global phenomenon, fuelled by the emergence of increasingly open and highly competitive global markets, heightened consumer expectations, and new technologies and convergence. Examples from the U.K., Norway, Australia, New Zealand and the United States were cited to indicate the spread of this trend. Canadian utilities, among them B.C. Hydro, Nova Scotia Power and the Alberta utilities being prime examples, also have restructured recently into separate generation, transmission, distribution and consumer service units to better react to changing market needs. Alliances with other utilities or competitors in the energy sector to pursue business opportunities outside traditional markets, are also not uncommon. Therefore, it was only a question of time for Ontario Hydro, the largest Canadian electric utility, to come under public pressure to follow the national and global trend. The MacDonald Committee recommendations provide the foundation for radical change for the industry. If and when implemented, they could have a major impact on how the electricity sector in Canada will operate in the years to come. Sound decisions by the Ontario government as to the appropriate regulatory model may also determine the province's ability to compete in the unfolding restructuring of electricity markets throughout North America

  4. Electric industry restructuring review

    Energy Technology Data Exchange (ETDEWEB)

    Slocum Hollis, S. [Duane Morris LLP, Washington, DC (United States)

    2004-07-01

    Restructuring of the electric power industry began in the early 1990's in many jurisdictions in the United States. Restructuring was an attempt to offer large industrial customers lower rates and freedom from regulation for generators and traditional public utilities. The move has gained most attention in the past two years as some utilities report high profits while others, such as Pacific Gas and Electric Co., the largest investor-owned utility in the United States, is in bankruptcy. The August 2003 blackout in the Midwest and Northeast United States and Canada also raised questions regarding electric reliability. The question now remains whether markets should be allowed to determine the need for services and the prices to be charged, and who is in charge in the imperfect market. The Federal Energy Regulatory Commission's (FERC) Order 2000 led to the formation of a Regional Transmission Organizations which is still in the implementation stage. Its influence on precursor Order numbers 888 and 889 were discussed in this paper with reference to independent system operators; regional transmission organizations; standard market design; rates and pricing devices; congestion management; market monitoring; market investigations; reliability measures; OASIS and other information access; interconnection policy; jurisdiction; mergers and merger policy; standards of conduct; policing affiliates; municipal utilities; stranded costs; and, state restructuring scorecards. refs.

  5. A radiochemical assay for glycolytic activity in dental plaque

    International Nuclear Information System (INIS)

    Williams, R.A.D.; Nuki, K.; Nanda, V.; Schlenker, R.

    1976-01-01

    A radiochemical technique for the rapid and precise measurement of glucose utilization of fresh samples of dental plaque is described. The method appears to be a sensitive indicator of the actual glycolytic ability of an organized microbial plaque including activity in the Embden-Meyerhof pathway as well as heterolactic fermentation and the Entner-Doudoroff pathway. It was found that the glycolytic rate of plaque associated with periodontal pockets was significantly higher than that for plaque not so associated. (author)

  6. Electricity restructuring : a comparative review. 2. ed.

    International Nuclear Information System (INIS)

    Trebilcock, M.J.

    2004-03-01

    Power generation, transmission and distribution are the 3 main components of the electricity market. For many years, most jurisdictions chose to vertically integrate these segments into government or private monopolies. Price controls or rate of return regulations were commonly imposed by governments to prevent the abuse of monopoly power. However, inefficiencies with these arrangements led to poor investment decisions. As a result, many jurisdictions re-evaluated the structure of electricity markets. Restructuring was motivated by the desire to shift investment risk from consumers or taxpayers to producers and investors. The introduction of competitive electricity generation markets created a new sector in the industry. This sector was the retailing or selling of electricity to end-users by retail intermediaries. Although restructuring of the industry should offer more efficient pricing and better-informed consumption and investment decisions, there are many challenges in electricity reform. These challenges include pricing by incumbent generators, transmitters and distributors; discriminatory network access by monopoly transmitters and distributors; inelasticity of electricity supply and demand at peak times; lack of real-time price notification and response by consumers; stranded costs; and, political and consumer resistance to increases in retail prices. The time it takes to get a new power plant online is another issues that complicates new entry in the generation sector. The results of restructuring in Ontario, California, United Kingdom, Pennsylvania, New Jersey, Maryland, Alberta, and Australia were examined. These jurisdictions are trying to find a balance between competition and regulation. 91 refs., 2 figs

  7. Restructuring Teachers' Work

    Directory of Open Access Journals (Sweden)

    Lisa Kirtman

    2002-05-01

    Full Text Available Despite repeated attempts to reform schools, teachers' work has remained surprisingly stable. The purpose of this study was to investigate implementation of a state-funded restructuring initiative that intended broad changes in teachers' professional roles. Sponsors of the founding legislation reasoned that changes in teachers' roles would contribute to higher student achievement. This study examined the question of whether and how this program of comprehensive whole-school change promoted changes in teachers' roles in school governance, collegial relations, and the classroom. Further, the study traced the relationship of these changes to one another, and weighed the likelihood that they had the capacity to affect core educational practices. Theoretically, this study is situated in the available literature on teachers' collegial relations; participation in shared decision making; and classroom roles, relationships and practice. Three elementary schools served as the sites for intensive qualitative data collection completed over a two-year period. The schools differed in geographic location (two urban, one rural, but all enrolled a racially, ethnically and linguistically diverse population of students, and more than half of the students in each school qualified for free or reduced price lunch. The study resulted in multiple types and sources of data on teachers' professional roles, including: observations in classrooms, collegial interactions, and governance situations; interviews with teachers (including teacher leaders, parents, administrators, and students; and documents pertaining to the restructuring plans and process. Findings show that changes in the three areas were achieved unevenly in the three schools. All three schools introduced changes in classroom practice and roles, ranging from the adoption of multi-age classrooms to more modest innovations in curriculum or instruction. In only one case were changes in professional roles outside

  8. Bulgarian electricity market restructuring

    International Nuclear Information System (INIS)

    Ganev, Peter

    2009-01-01

    The energy sector in Bulgaria has undergone major restructuring in recent years. It faces the dual challenges of achieving regulatory stability to attract private investors, and creating a functioning competition energy market. As of the EU Accession in 2007, Bulgaria has fully liberalized power and gas markets. The 2003 Energy Law establishes the energy sector legal framework and sets the basis for creation of a transparent and predictable regulatory environment where the key regulatory responsibilities are vested with the State Energy and Water Regulatory Commission (SEWRC). The energy sector experienced significant problems in the first half of 2007 due to lost production capacities and regulatory failures on the electricity market. Excess price regulations on the market of electricity supplies to household, coupled with insufficient liberalization of imports and exports, create unfavorable conditions for power producers and large electricity users. The energy regulator has tried to achieve several incompatible targets as of July 1, 2007 for maintaining low electricity prices for households in response to political pressure, low power generation prices amid rising input costs, and market opening in compliance with EU regulations. (author)

  9. Trademark Values in Corporate Restructuring

    OpenAIRE

    Torres, Fernando

    2007-01-01

    In corporate restructuring under Chapter 11, an asset valuation is a central task for both legal and financial reasons. In the area of intangible assets, however, generally accepted accounting principles (GAAP) do not reflect internally-generated assets such as brands, trademarks, and other intellectual property. In practice, arbitrary rules of thumb are used to fill this gap, and closure, liquidation, financing, and restructuring decisions are made on this basis. This paper reports the ...

  10. Non-metabolic functions of glycolytic enzymes in tumorigenesis.

    Science.gov (United States)

    Yu, X; Li, S

    2017-05-11

    Cancer cells reprogram their metabolism to meet the requirement for survival and rapid growth. One hallmark of cancer metabolism is elevated aerobic glycolysis and reduced oxidative phosphorylation. Emerging evidence showed that most glycolytic enzymes are deregulated in cancer cells and play important roles in tumorigenesis. Recent studies revealed that all essential glycolytic enzymes can be translocated into nucleus where they participate in tumor progression independent of their canonical metabolic roles. These noncanonical functions include anti-apoptosis, regulation of epigenetic modifications, modulation of transcription factors and co-factors, extracellular cytokine, protein kinase activity and mTORC1 signaling pathway, suggesting that these multifaceted glycolytic enzymes not only function in canonical metabolism but also directly link metabolism to epigenetic and transcription programs implicated in tumorigenesis. These findings underscore our understanding about how tumor cells adapt to nutrient and fuel availability in the environment and most importantly, provide insights into development of cancer therapy.

  11. ­La restructuration du réseau culturel français au quotidien The everyday reconstruction of French cultural network La reestructuración cotidiana de la red cultural francesa

    Directory of Open Access Journals (Sweden)

    Christophe Premat

    2013-03-01

    Full Text Available Il s’agit de comprendre les transformations des conditions du métier d’agent culturel à la lumière des réformes en cours du réseau culturel français. La restructuration des activités culturelles des Instituts est-elle le signe d’une privatisation de l’action culturelle extérieure de l’État ? La coopération culturelle serait-elle alors prise en charge par d’autres acteurs ? Afin de répondre à ces questions, l’article s’appuie sur une étude de cas, celle de l’Institut français de Stockholm, qui a abandonné une partie de ses activités au profit d’autres actions de coopération.The article tries to understand how current reforms of the French cultural network might transform the profession of cultural agent. The question is whether the restructuring of cultural institutes’ cultural activities reflects a privatization of the State’s external cultural action, and if other actors might then assume responsibility for cultural cooperation. It responds with a case study of the French cultural centre in Stockholm, which gave up a part of its activities to adopt other cooperation actions.En este trabajo tratamos de comprender las transformaciones de las condiciones del oficio de agente cultural a la luz de las reformas actuales en la red cultural francesa. ¿Es la reestructuración de las actividades culturales de los Institutos el signo de una privatización de la acción cultural exterior del Estado ? ¿Otros actores tomarían entonces en sus manos la cooperación cultural ? Con el fin de responder a esas preguntas, el artículo se apoya en un estudio de caso realizado por el Instituto francés de Estocolmo, el cual abandonó una parte de sus actividades para encargarse de otras acciones de cooperación.

  12. postmortem glycolytic metabousm in the skeletal muscles of ...

    African Journals Online (AJOL)

    the stunned animals compared with the anaesthetised ones, there isno apparent relation between the G-6,P04 levels and initial pH in the muscles of the stunned animals. This may be due to the non·steady state fluxes of hexose monophos· phates through the glycolytic cycle soon after the stunning trauma. Such high levels ...

  13. Study of glycolytic intermediates in hereditary elliptocytosis with thalassemia

    Directory of Open Access Journals (Sweden)

    Pavri Roshan

    1977-01-01

    Full Text Available Glycolytic intermediates like ATP, DPG and GSH have been studied in a family with. hereditary elliptocytosis and thalassemia. Results indicate a fall in ATP with a concomitant rise in DPG in the Patient. Findings are discussed in relation to other data.

  14. The glycolytic pathway of Trimastix pyriformis is an evolutionary mosaic

    Directory of Open Access Journals (Sweden)

    Silberman Jeffrey D

    2006-11-01

    Full Text Available Abstract Background Glycolysis and subsequent fermentation is the main energy source for many anaerobic organisms. The glycolytic pathway consists of ten enzymatic steps which appear to be universal amongst eukaryotes. However, it has been shown that the origins of these enzymes in specific eukaryote lineages can differ, and sometimes involve lateral gene transfer events. We have conducted an expressed sequence tag (EST survey of the anaerobic flagellate Trimastix pyriformis to investigate the nature of the evolutionary origins of the glycolytic enzymes in this relatively unstudied organism. Results We have found genes in the Trimastix EST data that encode enzymes potentially catalyzing nine of the ten steps of the glycolytic conversion of glucose to pyruvate. Furthermore, we have found two different enzymes that in principle could catalyze the conversion of phosphoenol pyruvate (PEP to pyruvate (or the reverse reaction as part of the last step in glycolysis. Our phylogenetic analyses of all of these enzymes revealed at least four cases where the relationship of the Trimastix genes to homologs from other species is at odds with accepted organismal relationships. Although lateral gene transfer events likely account for these anomalies, with the data at hand we were not able to establish with confidence the bacterial donor lineage that gave rise to the respective Trimastix enzymes. Conclusion A number of the glycolytic enzymes of Trimastix have been transferred laterally from bacteria instead of being inherited from the last common eukaryotic ancestor. Thus, despite widespread conservation of the glycolytic biochemical pathway across eukaryote diversity, in a number of protist lineages the enzymatic components of the pathway have been replaced by lateral gene transfer from disparate evolutionary sources. It remains unclear if these replacements result from selectively advantageous properties of the introduced enzymes or if they are neutral

  15. Electricity restructuring : acting on principles

    International Nuclear Information System (INIS)

    Down, E.; Hoover, G.; Howatson, A.; Rheaume, G.

    2003-01-01

    In the second briefing of this series, the authors explored public policy decisions and political intervention, and their effect on electricity restructuring. Continuous and vigilant regulatory oversight of the electricity industry in Canada is required. The need for improved public policy to reduce uncertainty for private investors who wish to enter the market was made clear using case studies from the United Kingdom, California, Alberta, and Ontario. Clarity and consistency must be the two guiding principles for public policy decisions and political intervention in the sector. By clarity, the authors meant that rules, objectives, and timelines of the restructuring process are clear to all market participants. Market rules, implementation, and consumer expectations must be consistent. refs., 3 figs

  16. Development and operation of interconnections in a restructuring context

    International Nuclear Information System (INIS)

    2003-01-01

    In many countries the electrical network is not fully interconnected and the best technical solution to achieve interconnection has to be found. At the same time the electricity industry is being restructured and interconnecting independent energy markets presents technical challenges. It is therefore timely to consider interconnection development and operation options: examine the benefits of interconnecting electrical networks and the development strategies, review the interconnection design options and the technologies available, identify the operational issues, the security problems of large interconnected systems, the protection issues, consider the impact of the restructuring of the electrical supply industry, assess the political, environmental and social implications of interconnections. reorganized in slovenia from 5-7 april 2004. (author)

  17. Essays on restructured electricity markets

    Science.gov (United States)

    Nicholson, Emma Leah

    This dissertation focuses on the performance of restructured electricity markets in the United States. In chapter 1, I study bidder-specific offer caps ("BSOCs") which are used to mitigate market power in three wholesale electricity markets. The price of electricity is determined through multi-unit uniform price auctions and BSOCs impose an upper limit, which is increasing in marginal cost, on each generator's bid. I apply BSOCs in both the uniform and discriminatory price auctions and characterize the equilibria in a two firm model with stochastic demand. BSOCs unambiguously increase expected production efficiency in the uniform price auction and they can increase the expected profit of the generator with the lower cap. Chapter 2, coauthored with Ramteen Sioshansi, Ph.D., compares two types of uniform price auction formats used in wholesale electricity markets, centrally committed markets and self committed markets. In centrally committed markets, generators submit two-part bids consisting of a fixed startup cost and a variable (per MWh) energy cost, and the auctioneer ensures that no generator operates at a loss. Generators in self committed markets must incorporate their startup costs into their one part energy bids. We derive Nash equilibria for both the centrally and self committed electricity markets in a model with two symmetric generators with nonconvex costs and deterministic demand. Using a numerical example, we demonstrate that if the caps on the bid elements are chosen appropriately, the two market designs are equivalent in terms of generator revenues and settlement costs. Regulators and prominent academic experts believe that electric restructuring polices have stifled investment in new generation capacity. In chapter 3 I seek to determine whether these fears are supported by empirical evidence. I examine both total investment in megawatts and the number of new investments across regions that adopted different electric restructuring policies to

  18. How restructuring river connectivity changes freshwater fish biodiversity and biogeography

    Science.gov (United States)

    Lynch, Heather J.; Campbell Grant, Evan H.; Muneepeerakul, Rachata; Arunachalam, Muthukumarasamy; Rodriguez-Iturbe, Ignacio; Fagan, William F.

    2011-05-01

    Interbasin water transfer projects, in which river connectivity is restructured via man-made canals, are an increasingly popular solution to address the spatial mismatch between supply and demand of fresh water. However, the ecological consequences of such restructuring remain largely unexplored, and there are no general theoretical guidelines from which to derive these expectations. River systems provide excellent opportunities to explore how network connectivity shapes habitat occupancy, community dynamics, and biogeographic patterns. We apply a neutral model (which assumes competitive equivalence among species within a stochastic framework) to an empirically derived river network to explore how proposed changes in network connectivity may impact patterns of freshwater fish biodiversity. Without predicting the responses of individual extant species, we find the addition of canals connecting hydrologically isolated river basins facilitates the spread of common species and increases average local species richness without changing the total species richness of the system. These impacts are sensitive to the parameters controlling the spatial scale of fish dispersal, with increased dispersal affording more opportunities for biotic restructuring at the community and landscape scales. Connections between isolated basins have a much larger effect on local species richness than those connecting reaches within a river basin, even when those within-basin reaches are far apart. As a result, interbasin canal projects have the potential for long-term impacts to continental-scale riverine communities.

  19. Oxidative and glycolytic skeletal muscles show marked differences in gene expression profile in Chinese Qingyuan partridge chickens.

    Directory of Open Access Journals (Sweden)

    Shu Jingting

    Full Text Available Oxidative and glycolytic myofibers have different structures and metabolic characteristics and their ratios are important in determining poultry meat quality. However, the molecular mechanisms underlying their differences are unclear. In this study, global gene expression profiling was conducted in oxidative skeletal muscle (obtained from the soleus, or SOL and glycolytic skeletal muscle (obtained from the extensor digitorum longus, or EDL of Chinese Qingyuan partridge chickens, using the Agilent Chicken Gene Expression Chip. A total of 1224 genes with at least 2-fold differences were identified (P < 0.05, of which 654 were upregulated and 570 were downregulated in SOL. GO, KEGG pathway, and co-expressed gene network analyses suggested that PRKAG3, ATP2A2, and PPARGC1A might play important roles in myofiber composition. The function of PPARGC1A gene was further validated. PPARGC1A mRNA expression levels were higher in SOL than in EDL muscles throughout the early postnatal development stages. In myoblast cells, shRNA knockdown of PPARGC1A significantly inhibited some muscle development and transition-related genes, including PPP3CA, MEF2C, and SM (P < 0.01 or P < 0.05, and significantly upregulated the expression of FWM (P < 0.05. Our study demonstrates strong transcriptome differences between oxidative and glycolytic myofibers, and the results suggest that PPARGC1A is a key gene involved in chicken myofiber composition and transition.

  20. United States electric industry : restructuring in review

    International Nuclear Information System (INIS)

    Slocum Hollis, S.

    2004-01-01

    This paper provides a detailed review of the United States electric power industry. The aim of the review was to clarify and better define current industry procedures and practices in light of significant and recent restructuring. In addition, recent bankruptcies and the power blackout in 2003 have raised concerns over industry practices. Issues concerning Independent System Operators (ISO) and regional transmission organizations were evaluated, with reference to an evolution and implementation of Regional Transmission Organization (RTO) policy, including a cost-benefit analysis. A background of RTO formations was provided with reference to consolidation, selection process and transfer of assets. Standard market design, network access and pricing issues were reviewed, as well as market and reliability concerns. Issues concerning affiliate treatment, shortages and the effect of sale of securities were presented. Various approaches to congestion management were examined, with examples from California and New England. Market monitoring issues, investigations and hearings were also examined, with examples and orders, including details of refunds. Measures to improve reliability were reviewed, including: management systems, benefit margins, requirements, assurance agreements and reserve markets. Issues concerning information access were presented, including: Open Access Same-time Information System (OASIS) requirements; tagging; standard business practices and protocols; and quarterly report practices and protocols. Interconnection policies were reviewed with reference to applicability, service options and pricing. The issue of variations was examined, with case examples concerning cost allocation, contract rights and treatment of specific costs. Jurisdiction issues concerning corporate realignments and power exchanges were presented, as well as specific services and state-federal relations. Issues concerning mergers and merger policy were also discussed, with reference

  1. United States electric industry : restructuring in review

    Energy Technology Data Exchange (ETDEWEB)

    Slocum Hollis, S. [Duane Morris LLP, Washington, DC (United States)

    2004-11-01

    This paper provides a detailed review of the United States electric power industry. The aim of the review was to clarify and better define current industry procedures and practices in light of significant and recent restructuring. In addition, recent bankruptcies and the power blackout in 2003 have raised concerns over industry practices. Issues concerning Independent System Operators (ISO) and regional transmission organizations were evaluated, with reference to an evolution and implementation of Regional Transmission Organization (RTO) policy, including a cost-benefit analysis. A background of RTO formations was provided with reference to consolidation, selection process and transfer of assets. Standard market design, network access and pricing issues were reviewed, as well as market and reliability concerns. Issues concerning affiliate treatment, shortages and the effect of sale of securities were presented. Various approaches to congestion management were examined, with examples from California and New England. Market monitoring issues, investigations and hearings were also examined, with examples and orders, including details of refunds. Measures to improve reliability were reviewed, including: management systems, benefit margins, requirements, assurance agreements and reserve markets. Issues concerning information access were presented, including: Open Access Same-time Information System (OASIS) requirements; tagging; standard business practices and protocols; and quarterly report practices and protocols. Interconnection policies were reviewed with reference to applicability, service options and pricing. The issue of variations was examined, with case examples concerning cost allocation, contract rights and treatment of specific costs. Jurisdiction issues concerning corporate realignments and power exchanges were presented, as well as specific services and state-federal relations. Issues concerning mergers and merger policy were also discussed, with reference

  2. Common phenotype of resting mouse extensor digitorum longus and soleus muscles: equal ATPase and glycolytic flux during transient anoxia.

    Science.gov (United States)

    Vinnakota, Kalyan C; Rusk, Joshua; Palmer, Lauren; Shankland, Eric; Kushmerick, Martin J

    2010-06-01

    Rates of ATPase and glycolysis are several times faster in actively contracting mouse extensor digitorum longus muscle (EDL) than soleus (SOL), but we find these rates are not distinguishable at rest. We used a transient anoxic perturbation of steady state energy balance to decrease phosphocreatine (PCr) reversibly and to measure the rates of ATPase and of lactate production without muscle activation or contraction. The rate of glycolytic ATP synthesis is less than the ATPase rate, accounting for the continual PCr decrease during anoxia in both muscles. We fitted a mathematical model validated with properties of enzymes and solutes measured in vitro and appropriate for the transient perturbation of these muscles to experimental data to test whether the model accounts for the results. Simulations showed equal rates of ATPase and lactate production in both muscles. ATPase controls glycolytic flux by feedback from its products. Adenylate kinase function is critical because a rise in [AMP] is necessary to activate glycogen phosphorylase. ATPase is the primary source of H+ production. The sum of contributions of the 13 reactions of the glycogenolytic and glycolytic network to total proton load is negligible. The stoichiometry of lactate and H+ production is near unity. These results identify a default state of energy metabolism for resting muscle in which there is no difference in the metabolic phenotype of EDL and SOL. Therefore, additional control mechanisms, involving higher ATPase flux and [Ca2+], must exist to explain the well-known difference in glycolytic rates in fast-twitch and slow-twitch muscles in actively contracting muscle.

  3. Coasts under stress: restructuring and social-ecological health

    National Research Council Canada - National Science Library

    Ommer, Rosemary E

    2007-01-01

    ... the human impact of restructuring and social-ecological health 9 The Restructuring of Health Care on Both Coasts since the 1980 s 183 The Statistical Face of Restructuring and Human Health 210 The Human Voice of Social-Ecological Restructuring: Jobs, Incomes, Livelihoods, Ways of Life, and Human Health 241vi Contents 10 11 Restructuring, Nutrition,...

  4. Electric industry restructuring in Michigan

    International Nuclear Information System (INIS)

    Anon.

    1997-01-01

    This Staff Report suggests a modified approach designed to significantly increase the ability of all customer classes to participate and share in the benefits of competition. The concepts discussed in this Report are designed to ensure that rates are not increased for any customers as a result of restructuring and, where possible, rates are reduced through the use of rate reduction bonds. The program outlined in this Report is designed to fulfill five objectives. First, it protects the interests of smaller customers, including low-income residential customers and senior citizens. Second, the program provides opportunities to strengthen Michigan's business community. Third, the program includes funding for employee retraining to assure that utility employees are not negatively impacted by restructuring. Fourth, the phase-in program provides the utilities with the opportunity to prepare for competition so that they remain Michigan-based companies. Fifth, the program is designed to foster competition upon a level playing field. The Commission has jurisdiction over all investor electric utilities and rural electric cooperatives in Michigan. Municipal electric utilities are not subject to Commission jurisdiction. Although this Report discusses details regarding Consumers Power and Detroit Edison, its concepts and principles are intended to apply to all jurisdictional electric utilities

  5. Role of Glycolytic Intermediates in Global Regulation and Signal Transduction. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Liao, J.C.

    2000-05-08

    The goal of this project is to determine the role of glycolytic intermediates in regulation of cell physiology. It is known that many glycolytic intermediates are involved in regulation of enzyme activities at the kinetic level. However, little is known regarding the role of these metabolites in global regulation and signal transduction. This project aims to investigate the role of glycolytic intermediates in the regulation of gene expression.

  6. Social Effects of Organisational Restructuring on Employee ...

    African Journals Online (AJOL)

    First Lady

    a health care organisation found that employees suffered poor morale and work satisfaction as a result of the necessary evil of creative destruction of organisational restructuring. In a Swedish study, Isaksson, Hellgreen and. Patterson, (2000) on repeated downsizing and other forms of restructuring in the retailing industry ...

  7. Reduction of Test Anxiety Through Cognitive Restructuring

    Science.gov (United States)

    Goldfried, Marvin R.; And Others

    1978-01-01

    On the basis of questionnaire measures of test anxiety, only those in the rational restructuring condition reported a significant decrease in subjective anxiety when placed in an analogue test-taking situation. Participants in the restructuring condition also reported greater generalized anxiety reduction in social-evaluative situations. (Author)

  8. Romanian refining industry assesses restructuring

    International Nuclear Information System (INIS)

    Tanasescu, D.G.

    1991-01-01

    The Romanian crude oil refining industry, as all the other economic sectors, faces the problems accompanying the transition from a centrally planned economy to a market economy. At present, all refineries have registered as joint-stock companies and all are coordinated and assisted by Rafirom S.A., from both a legal and a production point of view. Rafirom S.A. is a joint-stock company that holds shares in refineries and other stock companies with activities related to oil refining. Such activities include technological research, development, design, transportation, storage, and domestic and foreign marketing. This article outlines the market forces that are expected to: drive rationalization and restructuring of refining operations and define the targets toward which the reconfigured refineries should strive

  9. Enhanced oxidative stress and the glycolytic switch in superficial urothelial carcinoma of urinary bladder

    Directory of Open Access Journals (Sweden)

    Yu-Wei Lai

    2016-12-01

    Conclusions: UC of the UB manifested that the glycolytic phenotype would reflect the Warburg effect. We suggest that the molecular mechanism in the regulation of glycolytic switch in UC of the UB might provide a specific biomarker for the future development of cancer diagnosis.

  10. The restructuring of Africa's electricity sector : lessons learned

    International Nuclear Information System (INIS)

    Fall, A.

    2008-01-01

    The challenges facing the electricity sector in Africa was discussed with particular reference to financial constraints and the inefficient management of public utilities. The 2 types of reform in the electricity sector include the disengagement of the State for the benefit of the private sector, and the restructuring of the electrical industry to include competition for greater efficiency in the sector. The first reform in Africa involved the opening of the sector to private enterprises without the restructuring of the industry. With the exception of Uganda, the evolution of the restructuring did not include a complete de-integration in the transitional stage. Restructuring involved systematic independent production; a single buyer model with a limited period of monopoly on bulk purchase; access to third party on the networks; empowerment of regulation by the creation of an independent body responsible for regulation; and, establishment of a rural electrification agency. In more advanced countries such as Egypt, Algeria and Nigeria, the creation of a holding company with legally separated subsidiaries was retained. One of the major problems was financial investment, technical performances and the extension of electrical access. Low competition, or low negotiation capabilities led to unbalanced contracts in favour of private promoters. In general, rates for electrical energy increased due to the reform, with the lowering of public resources at the profit of private funding, notably in the independent production of thermal energy versus hydroelectric energy. The results have been mixed due to the breakdown of public versus private partnerships in many countries, with the disengagement of the State. Independent power production has raised problems of sustainability and environmental issues, as well as social problems, price control, as well as accessibility in rural settings. 7 refs., 1 fig

  11. Social Effects of Organisational Restructuring on Employee ...

    African Journals Online (AJOL)

    significant association between social effects of organisational restructuring and employee work-related attitudes. Furthermore, it was discovered that the general constellation of employee work -related attitudes, both the intrinsic and extrinsic ...

  12. Annual report on contractor work force restructuring, fiscal year 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    This report summarizes work force restructuring and community transition activities at all sites. It outlines work force restructuring activity for FY 1997, changing separation patterns, cost savings and separation costs, program assessment, activities to mitigate restructuring impacts, community transition activities, status of displaced workers, lessons learned, and emerging issues in worker and community transition. Work force restructuring and community transition activities for defense nuclear sites are summarized, as are work force restructuring activities at non-defense sites.

  13. Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor

    DEFF Research Database (Denmark)

    Lehning, Christina Eva; Siedler, Solvej; Ellabaan, Mostafa M Hashim

    2017-01-01

    We describe the development of an optimized glycolytic flux biosensor and its application in detecting altered flux in a production strain and in a mutant library. The glycolytic flux biosensor is based on the Cra-regulated ppsA promoter of E. coli controlling fluorescent protein synthesis. We...... validated the glycolytic flux dependency of the biosensor in a range of different carbon sources in six different E. coli strains and during mevalonate production. Furthermore, we studied the flux-altering effects of genome-wide single gene knock-outs in E. coli in a multiplex FlowSeq experiment. From...

  14. Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway

    Directory of Open Access Journals (Sweden)

    Dijon Henrique Salomé de Campos

    2014-10-01

    Full Text Available Background: Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Objective: Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced glucose leads to cardiac dysfunction in obesity. Methods: 30-day-old male Wistar rats were fed standard and hypercaloric diet for 30 weeks. Cardiac function and morphology were assessed. In this paper was viewed the general characteristics and comorbities associated to obesity. The structure cardiac was determined by weights of the heart and left ventricle (LV. Myocardial function was evaluated by studying isolated papillary muscles from the LV, under the baseline condition and after inotropic and lusitropic maneuvers: myocardial stiffness; postrest contraction; increase in extracellular Ca2+ concentration; change in heart rate and inhibitor of glycolytic pathway. Results: Compared with control group, the obese rats had increased body fat and co-morbities associated with obesity. Functional assessment after blocking iodoacetate shows no difference in the linear regression of DT, however, the RT showed a statistically significant difference in behavior between the control and the obese group, most notable being the slope in group C. Conclusion: The energy imbalance on obesity did not cause cardiac dysfunction. On the contrary, the prioritization of fatty acids utilization provides protection to cardiac muscle during the inhibition of glycolysis, suggesting that this pathway is fewer used by obese cardiac muscle.

  15. Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway

    Energy Technology Data Exchange (ETDEWEB)

    Campos, Dijon Henrique Salomé de, E-mail: dijoncampos@gmail.com [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Leopoldo, André Soares; Lima-Leopoldo, Ana Paula [Departamento de Esportes - Centro de Educação Física e Desportos da Universidade Federal do Espírito Santo (UFES), Vitória, ES (Brazil); Nascimento, André Ferreira do [Instituto de Ciências da Saúde da Universidade Federal do Mato Grosso (UFMT), Sinop, MT (Brazil); Oliveira-Junior, Silvio Assis de [Escola de Fisioterapia da Universidade Federal do Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil); Silva, Danielle Cristina Tomaz da [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Sugizaki, Mario Mateus [Instituto de Ciências da Saúde da Universidade Federal do Mato Grosso (UFMT), Sinop, MT (Brazil); Padovani, Carlos Roberto [Departamento de Bioestatística, Instituto de Ciências Biológicas da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Cicogna, Antonio Carlos, E-mail: dijoncampos@gmail.com [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil)

    2014-10-15

    Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced glucose leads to cardiac dysfunction in obesity. 30-day-old male Wistar rats were fed standard and hypercaloric diet for 30 weeks. Cardiac function and morphology were assessed. In this paper was viewed the general characteristics and comorbities associated to obesity. The structure cardiac was determined by weights of the heart and left ventricle (LV). Myocardial function was evaluated by studying isolated papillary muscles from the LV, under the baseline condition and after inotropic and lusitropic maneuvers: myocardial stiffness; postrest contraction; increase in extracellular Ca2+ concentration; change in heart rate and inhibitor of glycolytic pathway. Compared with control group, the obese rats had increased body fat and co-morbities associated with obesity. Functional assessment after blocking iodoacetate shows no difference in the linear regression of DT, however, the RT showed a statistically significant difference in behavior between the control and the obese group, most notable being the slope in group C. The energy imbalance on obesity did not cause cardiac dysfunction. On the contrary, the prioritization of fatty acids utilization provides protection to cardiac muscle during the inhibition of glycolysis, suggesting that this pathway is fewer used by obese cardiac muscle.

  16. Restructuring of RELAP5-3D

    Energy Technology Data Exchange (ETDEWEB)

    George Mesina; Joshua Hykes

    2005-09-01

    The RELAP5-3D source code is unstructured with many interwoven logic flow paths. By restructuring the code, it becomes easier to read and understand, which reduces the time and money required for code development, debugging, and maintenance. A structured program is comprised of blocks of code with one entry and exit point and downward logic flow. IF tests and DO loops inherently create structured code, while GOTO statements are the main cause of unstructured code. FOR_STRUCT is a commercial software package that converts unstructured FORTRAN into structured programming; it was used to restructure individual subroutines. Primarily it transforms GOTO statements, ARITHMETIC IF statements, and COMPUTED GOTO statements into IF-ELSEIF-ELSE tests and DO loops. The complexity of RELAP5-3D complicated the task. First, FOR_STRUCT cannot completely restructure all the complex coding contained in RELAP5-3D. An iterative approach of multiple FOR_STRUCT applications gave some additional improvements. Second, FOR_STRUCT cannot restructure FORTRAN 90 coding, and RELAP5-3D is partially written in FORTRAN 90. Unix scripts for pre-processing subroutines into coding that FOR_STRUCT could handle and post-processing it back into FORTRAN 90 were written. Finally, FOR_STRUCT does not have the ability to restructure the RELAP5-3D code which contains pre-compiler directives. Variations of a file were processed with different pre-compiler options switched on or off, ensuring that every block of code was restructured. Then the variations were recombined to create a completely restructured source file. Unix scripts were written to perform these tasks, as well as to make some minor formatting improvements. In total, 447 files comprising some 180,000 lines of FORTRAN code were restructured. These showed significant reduction in the number of logic jumps contained as measured by reduction in the number of GOTO statements and line labels. The average number of GOTO statements per subroutine

  17. Identification of a glycolytic regulon in the Archaea Pyrococcus and Thermococcus

    NARCIS (Netherlands)

    Werken, van de H.J.G.; Verhees, C.H.; Akerboom, A.P.; Vos, de W.M.; Oost, van der J.

    2006-01-01

    The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes,

  18. Role of the AMPKgamma3 isoform in hypoxia-stimulated glucose transport in glycolytic skeletal muscle

    DEFF Research Database (Denmark)

    Deshmukh, Atul S; Glund, Stephan; Tom, Robby Z

    2009-01-01

    in hypoxia-mediated energy status signaling and glucose transport in fast-twitch glycolytic extensor digitorum longus (EDL) muscle from AMPKgamma3-knockout (KO) mice and wild-type mice. Although hypoxia increased glucose transport (P

  19. Similar temperature dependencies of glycolytic enzymes : An evolutionary adaptation to temperature dynamics?

    NARCIS (Netherlands)

    Cruz, L.A.B.; Hebly, M.; Duong, G.H.; Wahl, S.A.; Pronk, J.T.; Heijnen, J.J.; Daran-Lapujade, P.; Van Gulik, W.M.

    2012-01-01

    Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in

  20. Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases

    DEFF Research Database (Denmark)

    Olsen, Lars Folke; Andersen, Ann Zahle; Lunding, Anita

    2009-01-01

    We investigated the coupling between glycolytic and mitochondrial membrane potential oscillations in Saccharomyces cerevisiae under semianaerobic conditions. Glycolysis was measured as NADH autofluorescence, and mitochondrial membrane potential was measured using the fluorescent dye 3,3'-diethylo...

  1. A quality roadmap of a restructured hospital.

    Science.gov (United States)

    Chow-Chua, C F; Goh, M

    1999-09-01

    This paper presents a quality roadmap of a restructured hospital. Specifically, a case study is showcased to reveal how a FOCUS-PDCA model is applied to the Central Portering Services (CPS) within a restructured hospital, to provide better service to other departments within the hospital who can then in turn value add to the patient care delivery chain. The preliminary results suggest that implementers of quality and service improvement programmes using the FOCUS-PDCA approach should note some of the key implementation difficulties. These difficulties occur at all levels and include issues relevant to change management, process improvement programmes, accessibility to data, communication and judicious execution of the change process.

  2. Uranium dioxide restructuration under irradiation. Furet program

    International Nuclear Information System (INIS)

    Stora, J.P.; Hueber, C.

    1975-01-01

    A complete model of fuel restructuration under irradiation was built on the basis of many short irradiation experiments carried out in Siloe at Grenoble between 1970 and 1973. From the experiments, carefully temperature and power controlled, a precise relationship was established between the microstructure and temperature conditions. The main points studied were: the type of recrystallization as a function of density; restructuration and displacements of matter tending to central hole formation; healing of cracks and thermal take-up of clearance and efficiency of thermal cycles and mechanical take-up of clearance [fr

  3. Synaptic Homeostasis and Restructuring across the Sleep-Wake Cycle.

    Directory of Open Access Journals (Sweden)

    Wilfredo Blanco

    2015-05-01

    Full Text Available Sleep is critical for hippocampus-dependent memory consolidation. However, the underlying mechanisms of synaptic plasticity are poorly understood. The central controversy is on whether long-term potentiation (LTP takes a role during sleep and which would be its specific effect on memory. To address this question, we used immunohistochemistry to measure phosphorylation of Ca2+/calmodulin-dependent protein kinase II (pCaMKIIα in the rat hippocampus immediately after specific sleep-wake states were interrupted. Control animals not exposed to novel objects during waking (WK showed stable pCaMKIIα levels across the sleep-wake cycle, but animals exposed to novel objects showed a decrease during subsequent slow-wave sleep (SWS followed by a rebound during rapid-eye-movement sleep (REM. The levels of pCaMKIIα during REM were proportional to cortical spindles near SWS/REM transitions. Based on these results, we modeled sleep-dependent LTP on a network of fully connected excitatory neurons fed with spikes recorded from the rat hippocampus across WK, SWS and REM. Sleep without LTP orderly rescaled synaptic weights to a narrow range of intermediate values. In contrast, LTP triggered near the SWS/REM transition led to marked swaps in synaptic weight ranking. To better understand the interaction between rescaling and restructuring during sleep, we implemented synaptic homeostasis and embossing in a detailed hippocampal-cortical model with both excitatory and inhibitory neurons. Synaptic homeostasis was implemented by weakening potentiation and strengthening depression, while synaptic embossing was simulated by evoking LTP on selected synapses. We observed that synaptic homeostasis facilitates controlled synaptic restructuring. The results imply a mechanism for a cognitive synergy between SWS and REM, and suggest that LTP at the SWS/REM transition critically influences the effect of sleep: Its lack determines synaptic homeostasis, its presence causes

  4. The Plasma Membrane Calcium Pump in Pancreatic Cancer Cells Exhibiting the Warburg Effect Relies on Glycolytic ATP.

    Science.gov (United States)

    James, Andrew D; Patel, Waseema; Butt, Zohra; Adiamah, Magretta; Dakhel, Raga; Latif, Ayse; Uggenti, Carolina; Swanton, Eileithyia; Imamura, Hiromi; Siriwardena, Ajith K; Bruce, Jason I E

    2015-10-09

    Evidence suggests that the plasma membrane Ca(2+)-ATPase (PMCA), which is critical for maintaining a low intracellular Ca(2+) concentration ([Ca(2+)]i), utilizes glycolytically derived ATP in pancreatic ductal adenocarcinoma (PDAC) and that inhibition of glycolysis in PDAC cell lines results in ATP depletion, PMCA inhibition, and an irreversible [Ca(2+)]i overload. We explored whether this is a specific weakness of highly glycolytic PDAC by shifting PDAC cell (MIA PaCa-2 and PANC-1) metabolism from a highly glycolytic phenotype toward mitochondrial metabolism and assessing the effects of mitochondrial versus glycolytic inhibitors on ATP depletion, PMCA inhibition, and [Ca(2+)]i overload. The highly glycolytic phenotype of these cells was first reversed by depriving MIA PaCa-2 and PANC-1 cells of glucose and supplementing with α-ketoisocaproate or galactose. These culture conditions resulted in a significant decrease in both glycolytic flux and proliferation rate, and conferred resistance to ATP depletion by glycolytic inhibition while sensitizing cells to mitochondrial inhibition. Moreover, in direct contrast to cells exhibiting a high glycolytic rate, glycolytic inhibition had no effect on PMCA activity and resting [Ca(2+)]i in α-ketoisocaproate- and galactose-cultured cells, suggesting that the glycolytic dependence of the PMCA is a specific vulnerability of PDAC cells exhibiting the Warburg phenotype. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  5. Restructured site characterization program at Yucca Mountain

    International Nuclear Information System (INIS)

    Dyer, J.R.; Vawter, R.G.

    1995-01-01

    During 1994 and the early part of 1995, the US Department of Energy's Yucca Mountain Site Characterization Office (YMSCO) and its parent organization, the Office of Civilian Radioactive Waste Management (OCRWM) underwent a significant restructuring. Senior Department officials provided the leadership to reorient the management, technical, programmatic, and public interaction approach to the US High Level Radioactive Waste Disposal Program. The restructuring involved reorganizing the federal staff, conducting meaningful strategic planning, improving the management system, rationalizing contractor responsibilities, focusing upon major products, and increasing stakeholder involvement. The restructured program has prioritized technical and scientific activities toward meeting major regulatory milestones in a timely and cost-effective manner. This approach has raised concern among elements of technical, scientific, and oversight bodies that suitability and licensing decisions could be made without obtaining sufficient technical information for this first-of-its-kind endeavor. Other organizations, such as congressional committees, industrial groups, and rate payers believe characterization goals can be met in a timely manner and within the limitation of available funds. To balance these contrasting views in its decision making process, OCRWM management has made a special effort to communicate its strategy to oversight bodies, the scientific community and other stakeholders and to use external independent peer review as a key means of demonstrating scientific credibility. Site characterization of Yucca Mountain in Nevada is one of the key elements of the restructured program

  6. Urban restructuring and deregulations in Latin America

    NARCIS (Netherlands)

    Carmona, M.

    1992-01-01

    With this work we continue the debate on the trends of urban restructuring in Latin America. In fact due to the large economic crisis of the 80-ies, the continent has decreased is living standards to that of 12 years ago. The productive potential has increased in 1,3% in circumstances that in the

  7. The first step in restructuring the US electric industry

    International Nuclear Information System (INIS)

    Barnett, A.H.; Reutter, K.A.; Thompson, H.

    2005-01-01

    The electric industry lobbied for its first step in restructuring, from local franchises to regulated state monopolies during the early 1900s. The present paper examines this restructuring with an empirical model that includes the introduction of natural gas and steam turbine generators. The restructuring resulted in fewer firms, higher costs, and higher prices, consistent with increased monopoly power and captured regulators. (author)

  8. The first step in restructuring the US electric industry

    Energy Technology Data Exchange (ETDEWEB)

    Barnett, A.H. [American University of Sharjah (United Arab Emirates); Reutter, K.A. [Nathan Associates, Auburn (United States); Thompson, H. [Auburn University (United States). Comer Hall

    2005-03-01

    The electric industry lobbied for its first step in restructuring, from local franchises to regulated state monopolies during the early 1900s. The present paper examines this restructuring with an empirical model that includes the introduction of natural gas and steam turbine generators. The restructuring resulted in fewer firms, higher costs, and higher prices, consistent with increased monopoly power and captured regulators. (author)

  9. 48 CFR 231.205-70 - External restructuring costs.

    Science.gov (United States)

    2010-10-01

    ... ownership or control. They do not include restructuring activities occurring after a business combination... control, or, when there has been no business combination, restructuring activities undertaken within one... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false External restructuring...

  10. On the functional vs. lexical nature of restructuring heads: evidence for a fine-grained classification of restructuring modals

    Directory of Open Access Journals (Sweden)

    Irene Balza

    2012-05-01

    Full Text Available This paper examines in detail the thematic and syntactic properties of a set of (non-restructuring structures in Germanic, Romance and Basque. Based on the comparison of these structures, I propose a fine-grained classification of (non-restructuring constructions that include Functional Restructuring, Semi-Lexical Restructuring, Lexical Restructuring and Non-Restructuring constructions. The four types involve complements of different underlying structures (VPs, vPs or even as large as NegP/TPs. The results of the analysis argue against Cinque’s (2005 hypothesis that all restructuring verbs are exclusively functional, and contradicts Wurmbrand’s (1999 claim that, within the mixed class of restructuring verbs, modals must be raising verbs.

  11. Evidence for loss of a partial flagellar glycolytic pathway during trypanosomatid evolution.

    Directory of Open Access Journals (Sweden)

    Robert W B Brown

    Full Text Available Classically viewed as a cytosolic pathway, glycolysis is increasingly recognized as a metabolic pathway exhibiting surprisingly wide-ranging variations in compartmentalization within eukaryotic cells. Trypanosomatid parasites provide an extreme view of glycolytic enzyme compartmentalization as several glycolytic enzymes are found exclusively in peroxisomes. Here, we characterize Trypanosoma brucei flagellar proteins resembling glyceraldehyde-3-phosphate dehydrogenase (GAPDH and phosphoglycerate kinase (PGK: we show the latter associates with the axoneme and the former is a novel paraflagellar rod component. The paraflagellar rod is an essential extra-axonemal structure in trypanosomes and related protists, providing a platform into which metabolic activities can be built. Yet, bioinformatics interrogation and structural modelling indicate neither the trypanosome PGK-like nor the GAPDH-like protein is catalytically active. Orthologs are present in a free-living ancestor of the trypanosomatids, Bodo saltans: the PGK-like protein from B. saltans also lacks key catalytic residues, but its GAPDH-like protein is predicted to be catalytically competent. We discuss the likelihood that the trypanosome GAPDH-like and PGK-like proteins constitute molecular evidence for evolutionary loss of a flagellar glycolytic pathway, either as a consequence of niche adaptation or the re-localization of glycolytic enzymes to peroxisomes and the extensive changes to glycolytic flux regulation that accompanied this re-localization. Evidence indicating loss of localized ATP provision via glycolytic enzymes therefore provides a novel contribution to an emerging theme of hidden diversity with respect to compartmentalization of the ubiquitous glycolytic pathway in eukaryotes. A possibility that trypanosome GAPDH-like protein additionally represents a degenerate example of a moonlighting protein is also discussed.

  12. Restructuring local distribution services: Possibilities and limitations

    Energy Technology Data Exchange (ETDEWEB)

    Duann, D.J.

    1994-08-01

    The restructuring of local distribution services is now the focus of the natural gas industry. It is the last major step in the ``reconstitution`` of the natural gas industry and a critical clement in realizing the full benefits of regulatory and market reforms that already have taken place in the wellhead and interstate markets. It could also be the most important regulatory initiative for most end-use customers because they are affected directly by the costs and reliability of distribution services. Several factors contribute to the current emphasis on distribution service restructuring. They include the unbundling and restructuring of upstream markets, a realization of the limitations of supply-side options (such as gas procurement oversight), and the increased diversity and volatility of gas demand facing local distribution companies. Local distribution service is not one but a series of activities that start with commodity gas procurement and extend to transportation, load balancing, storage, and metering and billing of services provided. There are also considerable differences in the economies of scale and scope associated with these various activities. Thus, a mixture of supply arrangements (such as a competitive market or a monopoly) is required for the most efficient delivery of local distribution services. A distinction must be made between the supply of commodity gas and the provision of a bundled distribution service. This distinction and identification of the best supply arrangements for various distribution service components are the most critical factors in developing appropriate restructuring policies. For most state public utility commissions the criteria for service restructuring should include pursuing the economies of scale and scope in gas distribution, differentiating and matching gas service reliability and quality with customer requirements, and controlling costs associated with the search, negotiation, and contracting of gas services.

  13. The glycolytic effect of 1-epinephrine and nor-epinephrine on the liver of the chick embryo.

    Science.gov (United States)

    Delphia, J M

    1976-10-01

    The glycolytic effect of 1-epinephrine and nor-epinephrine administered in-ovo for two hours on the liver of the chick embryo is reported. 1-epinephrine was more glycolytic than nor-epinephrine throughout the study. The glycolytic effect of 1-epinephrine was dosage-dependent throughout the study while nor-epinephrine brought about dosage-dependent liver-glycogen-depletion only in the nine day embryo.

  14. Status of power sector restructuring in Lithuania

    International Nuclear Information System (INIS)

    Bachiauskas, A.

    1999-01-01

    Lithuanian electricity sector comprises joint stock company Lietuvos Energija, which is responsible for supplying the country with electric energy, the Ignalina NPP state enterprise, which generates more then three fourth of the entire electricity of Lithuania, CHPs run By municipalities of Kaunas and Vilnius, and a few power plants, owned by industrial enterprises, as well as private small hydro power plants. Joint stock company Lietuvos Energija is a vertically integrated monopoly, which has inherited drawbacks inherent in planned - administrational economy and is therefore now being restructured into a modern and effective structure capable of operating within market economy conditions. The main goals of restructuring would meet electricity economy requirements: clearly un bundle electricity generation costs from electricity transmission and distribution costs; create internal and external electricity market conditions by gradual transition to wholesale and retail electricity trade; create conditions for third party access; create favourable conditions for competition; provide a possibility to integrate with Baltic electricity market in progress

  15. Services under siege--the restructuring imperative.

    Science.gov (United States)

    Roach, S S

    1991-01-01

    Recent job losses in the U.S. service sector do not reflect a temporary recession. Those jobs are gone, the result of a massive restructuring of the sector that is just getting under way. The explanation for the restructuring is quite simple. Until recently, services have been shielded by regulation and confronted by few foreign competitors. They have allowed their white-collar payrolls to become bloated, their investment in information technology to outstrip the paybacks, and their productivity to stagnate. Now competition is heating up and exposing these inefficiencies. Just as intense competition forced the restructuring of Smokestack America in the 1980s, deregulation and foreign direct investment are shaking out service companies that cannot confront their shortcomings. The need for sweeping change in the service sector may come as a great shock to Americans who saw services as the means to continued economic prosperity. But there is a painful irony at work: job creation, the very thing proponents use to demonstrate the U.S. service sector's strength, is in fact a symptom of the sector's chronic neglect of economic efficiency. It is precisely that neglect that makes the service sector vulnerable as the race for market share intensifies and new players shift the terms of competition. Services must respond to the new competitive environment, but not by indiscriminate cost cutting. Instead, they should balance financial discipline with a comprehensive and immediate reexamination of strategy.

  16. Synchronization of glycolytic oscillations in a yeast cell population

    DEFF Research Database (Denmark)

    Dano, S.; Hynne, F.; De Monte, Silvia

    2001-01-01

    The mechanism of active phase synchronization in a suspension of oscillatory yeast cells has remained a puzzle for almost half a century. The difficulty of the problem stems from the fact that the synchronization phenomenon involves the entire metabolic network of glycolysis and fermentation, and...

  17. Preslaughter handling effects on pork quality and glycolytic potential in two muscles differing in fiber type composition

    NARCIS (Netherlands)

    Hambrecht, E.; Eissen, J.J.; Newman, D.J.; Smits, C.H.M.; Verstegen, M.W.A.; Hartog, den L.A.

    2005-01-01

    The objective of the present experiment was to investigate the effects of transportation, lairage, and preslaughter stressor treatment on glycolytic potential and pork quality of the glycolytic longissimus and the oxidative supraspinatus (SSP) or serratus ventralis (SV) muscles. In a 2 x 2 x 2

  18. The extent to which ATP demand controls the glycolytic flux depends strongly on the organism and conditions for growth

    DEFF Research Database (Denmark)

    Købmann, Brian Jensen; Westerhoff, H.V.; Snoep, J.L.

    2002-01-01

    Using molecular genetics we have introduced uncoupled ATPase activity in two different bacterial species, Escherichia coli and Lactococcus lactis, and determined the elasticities of the growth rate and glycolytic flux towards the intracellular [ATP]/[ADP] ratio. During balanced growth in batch...... cultures of E. coli the ATP demand was found to have almost full control on the glycolytic flux (FCC=0.96) and the flux could be stimulated by 70%. In contrast to this, in L. lactis the control by ATP demand on the glycolytic flux was close to zero. However, when we used non-growing cells of L. lactis...... (which have a low glycolytic flux) the ATP demand had a high flux control and the flux could be stimulated more than two fold. We suggest that the extent to which ATP demand controls the glycolytic flux depends on how much excess capacity of glycolysis is present in the cells....

  19. Primordial oscillations in life: Direct observation of glycolytic oscillations in individual HeLa cervical cancer cells

    Science.gov (United States)

    Amemiya, Takashi; Shibata, Kenichi; Itoh, Yoshihiro; Itoh, Kiminori; Watanabe, Masatoshi; Yamaguchi, Tomohiko

    2017-10-01

    We report the first direct observation of glycolytic oscillations in HeLa cervical cancer cells, which we regard as primordial oscillations preserved in living cells. HeLa cells starved of glucose or both glucose and serum exhibited glycolytic oscillations in nicotinamide adenine dinucleotide (NADH), exhibiting asynchronous intercellular behaviors. Also found were spatially homogeneous and inhomogeneous intracellular NADH oscillations in the individual cells. Our results demonstrate that starved HeLa cells may be induced to exhibit glycolytic oscillations by either high-uptake of glucose or the enhancement of a glycolytic pathway (Crabtree effect or the Warburg effect), or both. Their asynchronous collective behaviors in the oscillations were probably due to a weak intercellular coupling. Elucidation of the relationship between the mechanism of glycolytic dynamics in cancer cells and their pathophysiological characteristics remains a challenge in future.

  20. Contribution of glucose transport to the control of the glycolytic flux in Trypanosoma brucei.

    NARCIS (Netherlands)

    Bakker, B.M.; Walsh, M.C.; ter Kuile, B.; Mensonides, F.I.C.; Michels, P.A.M.; Opperdoes, F.R.; Westerhoff, H.V.

    1999-01-01

    The rate of glucose transport across the plasma membrane of the bloodstream form of Trypanosoma brucei was modulated by titration of the hexose transporter with the inhibitor phloretin, and the effect on the glycolytic flux was measured. A rapid glucose uptake assay was developed to measure the

  1. Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes

    Directory of Open Access Journals (Sweden)

    S Andrea Moreno

    2015-06-01

    Full Text Available Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison. Herein, we provide the first report on the subcellular localisation of the glycolytic enzymes in T. evansi, which is a alike to that of the bloodstream form (BSF of T. b. brucei: (i fructose-bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH, hexokinase, phosphofructokinase, glucose-6-phosphate isomerase, phosphoglycerate kinase, triosephosphate isomerase (glycolytic enzymes and glycerol-3-phosphate dehydrogenase (a glycolysis-auxiliary enzyme in glycosomes, (ii enolase, phosphoglycerate mutase, pyruvate kinase (glycolytic enzymes and a GAPDH isoenzyme in the cytosol, (iii malate dehydrogenase in cytosol and (iv glucose-6-phosphate dehydrogenase in both glycosomes and the cytosol. Specific enzymatic activities also suggest that T. evansi is alike to the BSF of T. b. brucei in glycolytic flux, which is much faster than the pentose phosphate pathway flux, and in the involvement of cytosolic GAPDH in the NAD+/NADH balance. These similarities were expected based on the close phylogenetic relationship of both parasites.

  2. Impact of Autoantibodies against Glycolytic Enzymes on Pathogenicity of Autoimmune Retinopathy and Other Autoimmune Disorders

    Directory of Open Access Journals (Sweden)

    Grazyna Adamus

    2017-04-01

    Full Text Available Autoantibodies (AAbs against glycolytic enzymes: aldolase, α-enolase, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate kinase are prevalent in sera of patients with blinding retinal diseases, such as paraneoplastic [cancer-associated retinopathy (CAR] and non-paraneoplastic autoimmune retinopathies, as well as in many other autoimmune diseases. CAR is a degenerative disease of the retina characterized by sudden vision loss in patients with cancer and serum anti-retinal AAbs. In this review, we discuss the widespread serum presence of anti-glycolytic enzyme AAbs and their significance in autoimmune diseases. There are multiple mechanisms responsible for antibody generation, including the innate anti-microbial response, anti-tumor response, or autoimmune response against released self-antigens from damaged, inflamed tissue. AAbs against enolase, GADPH, and aldolase exist in a single patient in elevated titers, suggesting their participation in pathogenicity. The lack of restriction of AAbs to one disease may be related to an increased expression of glycolytic enzymes in various metabolically active tissues that triggers an autoimmune response and generation of AAbs with the same specificity in several chronic and autoimmune conditions. In CAR, the importance of serum anti-glycolytic enzyme AAbs had been previously dismissed, but the retina may be without pathological consequence until a failure of the blood–retinal barrier function, which would then allow pathogenic AAbs access to their retinal targets, ultimately leading to damaging effects.

  3. Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast

    NARCIS (Netherlands)

    Zampar, Guillermo G; Kümmel, Anne; Ewald, Jennifer; Jol, Stefan; Niebel, Bastian; Picotti, Paola; Aebersold, Ruedi; Sauer, Uwe; Zamboni, Nicola; Heinemann, Matthias

    The diauxic shift in Saccharomyces cerevisiae is an ideal model to study how eukaryotic cells readjust their metabolism from glycolytic to gluconeogenic operation. In this work, we generated time-resolved physiological data, quantitative metabolome (69 intracellular metabolites) and proteome (72

  4. Development of an intracellular glycolytic flux sensor for high throughput applications in E.coli

    DEFF Research Database (Denmark)

    Lehning, Christina Eva

    The aim of this PhD project was to construct, test and apply an intracellular, growth-­‐ independent and direct measureable glycolytic flux biosensor in E. coli. Studying the metabolic flux of bacterial cells is of growing interest as it is of fundamental importance to bacterial physiology as wel...

  5. Restructuring in Turkish power generation and transmission

    International Nuclear Information System (INIS)

    Dilli, B.

    2001-01-01

    Restructuring of power sector is an evolutionary process and it is in its early days. As the reform proceeds and the market evolve and become more efficient, a number of unresolved issues shall be overcomed through re-regulations. So, the most important point is the smooth transition to the liberalized markets. The solution is dependent upon the assimilation, acceptation and supporting of the reform by the parties concerned. Market participants' acceptance and actions will be the key element for the success of the new market. (author)

  6. Optimization Specifications for CUDA Code Restructuring Tool

    KAUST Repository

    Khan, Ayaz

    2017-03-13

    In this work we have developed a restructuring software tool (RT-CUDA) following the proposed optimization specifications to bridge the gap between high-level languages and the machine dependent CUDA environment. RT-CUDA takes a C program and convert it into an optimized CUDA kernel with user directives in a configuration file for guiding the compiler. RTCUDA also allows transparent invocation of the most optimized external math libraries like cuSparse and cuBLAS enabling efficient design of linear algebra solvers. We expect RT-CUDA to be needed by many KSA industries dealing with science and engineering simulation on massively parallel computers like NVIDIA GPUs.

  7. Deregulation-restructuring: Evidence for individual industries

    Energy Technology Data Exchange (ETDEWEB)

    Costello, K.W.; Graniere, R.J.

    1997-05-01

    Several studies have measured the effects of regulation on a particular industry. These studies range widely in sophistication, from simple observation (comparison) of pre-transformation and post-transformation actual industry performance to econometric analysis that attempt to separate the effects of deregulation from other factors in explaining changes in an industry`s performance. The major problem with observation studies is that they are unable to measure the effect of one particular event, such as deregulation, on an industry`s performance. For example, at the same time that the United Kingdom privatized its electric power industry, it also radically restructured the industry to encourage competition and instituted a price-cap mechanism to regulate the prices of transmission, distribution, and bundled retail services. Subsequent to these changes in 1991, real prices for most UK electricity customers have fallen. It is not certain however, which of these factors was most important or even contributed to the decline in price. In any event, one must be cautious in interpreting the results of studies that attempt to measure the effect of deregulation per se for a specific industry. This report highlights major outcomes for five industries undergoing deregulation or major regulatory and restructuring reforms. These include the natural gas, transportation, UK electric power, financial, and telecommunications industries. Particular attention was given to the historical development of events in the telecommunications industry.

  8. Scenarios for a deregulated, restructured electric industry

    International Nuclear Information System (INIS)

    Ford, G.C.

    1998-01-01

    Deregulation is moving rapidly on some fronts, while opposition by some affected parties is growing. The restructured industry is emerging, in the growth of new businesses such as power marketing and energy services, and also in reorganization by utilities to prepare for the future under deregulation. The market levels and opportunities will depend not only on the decisions of individual state and Federal regulators, but also on those of utilities, power marketers, developers and others who can drive the direction and the outcome of the changes. Scenarios for deregulation and restructuring are examined, from a rapid pace toward full competition, to a process slowed or blocked by conflicting interests and opposition from parties bent on preserving existing positions. A key development already underway with expected major impact on the future of the industry will be major consolidations, including convergence of electric and gas supply, and emergence of very large and powerful players. The generation business and energy supply to customers, based on aggressive marketing will lead the way

  9. An introduction to electric industry restructuring

    Energy Technology Data Exchange (ETDEWEB)

    Eisenberg, J.F.; Berry, L.G.

    1997-09-01

    This paper briefly describes the electric industry, its residential markets, industry structure and current trends. Its purpose is to provide Weatherization grants managers with the background necessary to assess their leveraging opportunities in an industry that is experiencing sweeping changes, commonly known as electric industry restructuring. The study describes the terrain of a changing industry topography on a national and regional basis, with some state and local information also provided. Weatherization managers and subgrantees who read this paper should be better able to understand the leveraging opportunities that are emerging now in the electricity market place. The reader will be introduced to the basics of the electric industry as it presently operates, the nature of the changes that are in the process of occurring, and the driving forces that are behind those changes. The major industry players are described by type and their interests are explored in further depth. There will also be an overview of the regulatory process as it has operated historically, as well as the changes now underway at both the state and federal levels. Finally, the paper will conclude with a description of some of the assets and opportunities available to those who may be interested in participating in the restructuring process in order to expand or protect low-income programs in their own states.

  10. Towards Restructuring Library and Information Science Curricula for ...

    African Journals Online (AJOL)

    This paper deals with restructuring of library and information science curriculum for the challenge of ICT environment. The paper highlights the following areas: conceptual framework, qualities of Library and Information Science curriculum, rationale for restructuring library and information science curriculum, areas in library ...

  11. Load frequency control of an asynchronous restructured power ...

    African Journals Online (AJOL)

    user

    This paper presents the analysis of load frequency control (LFC) of a two-area restructured power system interconnected via parallel ac/dc ... Keywords: Asynchronous tie-lines; Restructured power system; HVDC transmission links; Load frequency control; Fuzzy Logic ..... Automation, and Systems, Vol.4, No.2, pp.155-164.

  12. Serdemetan antagonizes the Mdm2-HIF1α axis leading to decreased levels of glycolytic enzymes.

    Directory of Open Access Journals (Sweden)

    Jason A Lehman

    Full Text Available Serdemetan (JNJ-26854165, an antagonist to Mdm2, was anticipated to promote the activation of p53. While regulation of p53 by Mdm2 is important, Mdm2 also regulates numerous proteins involved in diverse cellular functions. We investigated if Serdemetan would alter the Mdm2-HIF1α axis and affect cell survival in human glioblastoma cells independently of p53. Treatment of cells with Serdemetan under hypoxia resulted in a decrease in HIF1α levels. HIF1α downstream targets, VEGF and the glycolytic enzymes (enolase, phosphoglycerate kinase1/2, and glucose transporter 1, were all decreased in response to Serdemetan. The involvement of Mdm2 in regulating gene expression of glycolytic enzymes raises the possibility of side effects associated with therapeutically targeting Mdm2.

  13. An empirical examination of restructured electricity prices

    International Nuclear Information System (INIS)

    Knittel, C.R.; Roberts, M.R.

    2005-01-01

    We present an empirical analysis of restructured electricity prices. We study the distributional and temporal properties of the price process in a non-parametric framework, after which we parametrically model the price process using several common asset price specifications from the asset-pricing literature, as well as several less conventional models motivated by the peculiarities of electricity prices. The findings reveal several characteristics unique to electricity prices including several deterministic components of the price series at different frequencies. An 'inverse leverage effect' is also found, where positive shocks to the price series result in larger increases in volatility than negative shocks. We find that forecasting performance in dramatically improved when we incorporate features of electricity prices not commonly modelled in other asset prices. Our findings have implications for how empiricists model electricity prices, as well as how theorists specify models of energy pricing. (author)

  14. MCT4 Defines a Glycolytic Subtype of Pancreatic Cancer with Poor Prognosis and Unique Metabolic Dependencies

    Directory of Open Access Journals (Sweden)

    GuemHee Baek

    2014-12-01

    Full Text Available KRAS mutation, which occurs in ∼95% of pancreatic ductal adenocarcinoma (PDA, has been shown to program tumor metabolism. MCT4 is highly upregulated in a subset of PDA with a glycolytic gene expression program and poor survival. Models with high levels of MCT4 preferentially employ glycolytic metabolism. Selectively in such “addicted” models, MCT4 attenuation compromised glycolytic flux with compensatory induction of oxidative phosphorylation and scavenging of metabolites by macropinocytosis and autophagy. In spite of these adaptations, MCT4 depletion induced cell death characterized by elevated reactive oxygen species and metabolic crisis. Cell death induced by MCT4-depletion was augmented by inhibition of compensatory pathways. In xenograft models, MCT4 had a significant impact on tumor metabolism and was required for rapid tumor growth. Together, these findings illustrate the metabolic diversity of PDA described by MCT4, delineate pathways through which this lactate transporter supports cancer growth, and demonstrate that PDA can be rationally targeted based on metabolic addictions.

  15. Glycolytic control of vacuolar-type ATPase activity: A mechanism to regulate influenza viral infection

    International Nuclear Information System (INIS)

    Kohio, Hinissan P.; Adamson, Amy L.

    2013-01-01

    As new influenza virus strains emerge, finding new mechanisms to control infection is imperative. In this study, we found that we could control influenza infection of mammalian cells by altering the level of glucose given to cells. Higher glucose concentrations induced a dose-specific increase in influenza infection. Linking influenza virus infection with glycolysis, we found that viral replication was significantly reduced after cells were treated with glycolytic inhibitors. Addition of extracellular ATP after glycolytic inhibition restored influenza infection. We also determined that higher levels of glucose promoted the assembly of the vacuolar-type ATPase within cells, and increased vacuolar-type ATPase proton-transport activity. The increase of viral infection via high glucose levels could be reversed by inhibition of the proton pump, linking glucose metabolism, vacuolar-type ATPase activity, and influenza viral infection. Taken together, we propose that altering glucose metabolism may be a potential new approach to inhibit influenza viral infection. - Highlights: • Increased glucose levels increase Influenza A viral infection of MDCK cells. • Inhibition of the glycolytic enzyme hexokinase inhibited Influenza A viral infection. • Inhibition of hexokinase induced disassembly the V-ATPase. • Disassembly of the V-ATPase and Influenza A infection was bypassed with ATP. • The state of V-ATPase assembly correlated with Influenza A infection of cells

  16. Extracellular functions of glycolytic enzymes of parasites: unpredicted use of ancient proteins.

    Science.gov (United States)

    Gómez-Arreaza, Amaranta; Acosta, Hector; Quiñones, Wilfredo; Concepción, Juan Luis; Michels, Paul A M; Avilán, Luisana

    2014-02-01

    In addition of their usual intracellular localization where they are involved in catalyzing reactions of carbohydrate and energy metabolism by glycolysis, multiple studies have shown that glycolytic enzymes of many organisms, but notably pathogens, can also be present extracellularly. In the case of parasitic protists and helminths, they can be found either secreted or attached to the surface of the parasites. At these extracellular localizations, these enzymes have been shown to perform additional, very different so-called "moonlighting" functions, such as acting as ligands for a variety of components of the host. Due to this recognition, different extracellular glycolytic enzymes participate in various important parasite-host interactions such as adherence and invasion of parasites, modulation of the host's immune and haemostatic systems, promotion of angiogenesis, and acquisition of specific nutrients by the parasites. Accordingly, extracellular glycolytic enzymes are important for the invasion of the parasites and their establishment in the host, and in determining their virulence. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. Ten Australian ICU nurses' perceptions of organisational restructuring.

    Science.gov (United States)

    Wynne, Rochelle

    2004-02-01

    The Australian healthcare system underwent radical reform in the 1990s as economic rationalist policies were embraced. As a result, there was significant organisational restructuring within hospitals. Traditional indicators, such as nursing absenteeism and attrition, increase during times of organisational change. Despite this, nurses' views of healthcare reform are under-represented in the literature and little is known about the impact of organisational restructuring on perceived performance. This study investigated the perceived impact of organisational restructuring on a group of intensive care unit (ICU) nurses' workplace performance. It employed a qualitative approach to collect data from a purposive sample of clinical nurses. The primary method of data collection was semi-structured interviews. Content analysis generated three categories of data. Participants identified constant pressure, inadequate communication and organisational components of restructuring within the hospital as issues that had a significant impact on their workplace performance. They perceived organisational restructuring was poorly communicated, and this resulted in an environment of constant pressure. Organisational components of restructuring included the subcategories of specialised service provision and an alternative administrative structure that had both positive and negative ramifications for performance. To date, there has been little investigation of nurses' perceptions of organisational restructure or the impact this type of change has in the clinical domain. Participants in this study believed reorganisation was detrimental to quality care delivery in intensive care, as a result of fiscal constraint, inadequate communication and pressure that influenced their workplace performance.

  18. 77 FR 14979 - Transportation Conformity Rule Restructuring Amendments

    Science.gov (United States)

    2012-03-14

    ... conformity SIPs, see EPA's ``Guidance for Developing Transportation Conformity State Implementation Plans... January 2009 guidance entitled, ``Guidance for Developing Transportation Conformity State Implementation... Transportation Conformity Rule Restructuring Amendments AGENCY: Environmental Protection Agency (EPA). ACTION...

  19. 75 FR 55635 - Restructuring of the Stationary Source Audit Program

    Science.gov (United States)

    2010-09-13

    ... analysis that would include the sampling technique, sample handling, sample preparation, and sample... Part II Environmental Protection Agency 40 CFR Parts 51, 60, 61, et al. Restructuring of the..., 2010 / Rules and Regulations#0;#0; [[Page 55636

  20. RESTRUCTURED ELECTRICITY MARKETS: Three States' Experiences in Adding Generating Capacity

    National Research Council Canada - National Science Library

    2002-01-01

    ...., restructured electricity markets by shifting from service provided through a regulated monopoly-the local electric utility-to service provided through open competition among the local utility and its competitors...

  1. Academic Leadership and the Restructuring of Higher Education

    Science.gov (United States)

    Rich, Daniel

    2006-01-01

    The key challenge to academic leadership is to restructure the allocation of academic assets, particularly the organization of the faculty, in ways that better serve emerging societal and scholarly needs.

  2. SOME ASPECTS REGARDING THE RESTRUCTURING OPERATIONS OF THE

    Directory of Open Access Journals (Sweden)

    DRAGOS (COMAN DORICA

    2015-08-01

    Full Text Available The aim of this paper is the studying of the restructuring operations of the economical entities in Romania. Based on scientific observation, analysis and synthesis, the research starts with a presentation of the juridical frame of the restructuring operations in Romania, focusing further on analyzing of the two types of operations: the fusion between companies with negative capitals, and the operations made during the retroactivity period. Using the case study method, the study accomplishes a presentation of the main aspects of the restructuring operations of five economical entities in Romania: SC Astral Telecom SA, SC Romania Data Systems SA, SC Romconstruct Holding Grup SA, SC Artima Retail Investment Company SA and SC UPC Romania SA. The results of the research wish to underline the main aspects of the restructuring operations of the trading companies in Romania.

  3. Restructuring EPA's Libraries: Background and Issues for Congress

    National Research Council Canada - National Science Library

    Bearden, David M; Esworthy, Robert

    2007-01-01

    ..., $2.0 million of which was attributed mainly to these closures. EPA reports that the closings are part of its efforts to restructure its libraries to respond to the increasing use of the Internet to access its collections...

  4. Electricity distribution industry restructuring in South Africa: A case study

    Energy Technology Data Exchange (ETDEWEB)

    Gaunt, C.T. [University of Cape Town, Rondebosch (South Africa)

    2008-09-15

    For 20 years there has been vigorous debate about restructuring the large, developed electricity industry in South Africa, but the plans have not been implemented. The justification of the proposed restructuring illustrates the different expectations of the participants in the electricity industry. The case study reviews what has occurred against a background of some theories about organisations, and identifies six key issues. Some lessons include the need to adopt an industry structure compatible with the objectives of electricity distribution in developing countries, the unsuitability of orthodox approaches to organisations, that no industry structure appears to be significantly better than others, industry restructuring of public sector utilities has a significant political dimension, restructuring can fail even if there is support from a high level of government, and indecision and uncertainty have serious negative consequences. The experience should be helpful for other developing countries considering structural change of their electricity industry. (author)

  5. Electricity distribution industry restructuring in South Africa: A case study

    International Nuclear Information System (INIS)

    Gaunt, C.T.

    2008-01-01

    For 20 years there has been vigorous debate about restructuring the large, developed electricity industry in South Africa, but the plans have not been implemented. The justification of the proposed restructuring illustrates the different expectations of the participants in the electricity industry. The case study reviews what has occurred against a background of some theories about organisations, and identifies six key issues. Some lessons include the need to adopt an industry structure compatible with the objectives of electricity distribution in developing countries, the unsuitability of orthodox approaches to organisations, that no industry structure appears to be significantly better than others, industry restructuring of public sector utilities has a significant political dimension, restructuring can fail even if there is support from a high level of government, and indecision and uncertainty have serious negative consequences. The experience should be helpful for other developing countries considering structural change of their electricity industry

  6. The Transatlantic Defense Industrial Base: Restructuring Scenarios and Their Implications

    National Research Council Canada - National Science Library

    Guay, Terrence R

    2005-01-01

    This monograph compares the post-Cold War restructuring of the defense industries in the United States and Europe with the aim of understanding the implications for the transatlantic industrial base...

  7. The restructuring of the Russian oil industry

    International Nuclear Information System (INIS)

    Price, J.

    1994-01-01

    The Russian Oil and Gas Corporation, Rosneftegas, was set up in October 1990 to be the central coordinating body of the Russian oil industry. Rosneftegas decided to seek expert advice from international advisers on the restructuring of the industry in the transition to a market economy. The advisers put forward a framework for change based on eight internal and external factors. On the internal, industry, side they are: private ownership, market structures, competition and demarcation of responsibilities. On the external, policy side they are: oil and gas legislation; taxation; contracts; and pricing. A list of investment policy and industry structure objectives was defined on the basis of the framework. Much progress has been made and many of the detailed conclusions of the advisers' report have been implemented. Many difficult issues remain to be dealt with, however. These include considerable tension between the Russian Federal government and the regional governments, between production associations and their local governments, and over the domestic price of oil and oil products. (UK)

  8. Modular load flow for restructured power systems

    CERN Document Server

    Hariharan, M V; Gupta, Pragati P

    2016-01-01

    In the subject of power systems, authors felt that a re-look is necessary at some conventional methods of analysis. In this book, the authors have subjected the time-honoured load flow to a close scrutiny. Authors have discovered and discussed a new load flow procedure – Modular Load Flow. Modular Load Flow explores use of power – a scalar – as source for electrical circuits which are conventionally analysed by means of phasors – the ac voltages or currents. The method embeds Kirchhoff’s circuit laws as topological property into its scalar equations and results in a unique wonderland where phase angles do not exist! Generators are shown to have their own worlds which can be superimposed to obtain the state of the composite power system. The treatment is useful in restructured power systems where stakeholders and the system operators may desire to know individual generator contributions in line flows and line losses for commercial reasons. Solution in Modular Load Flow consists of explicit expression...

  9. Restructuring and privatization in energy sector

    International Nuclear Information System (INIS)

    Stojchev, D.; Pyrvanov, V.

    1994-01-01

    The ways of solving problems of the transition period to market economy are discussed. The current conditions in Bulgarian energy sector are defined taking into account different processes, stages, elements, objects. The criteria of the transition -economical. technological, organizational, social, ecological -and the problems - unemployment, requalification, privatization, contamination - are postulated. The recent experience of Bulgaria and other ex-communist countries in restructuring and privatization of the economy are considered. The scope of suitable approaches, methods, means and rates are outlined. The mechanisms of the tackled processes are analyzed by comparative investigation and management ways for impact on different levels are looked for. The possible consequences of given situation, advantages and shortcomings of different alternatives are formulated. The ways for assessment and selection of compromise solutions are proposed. An overall technology for assessment and application of different ways of transition is discussed. Their tools for business estimation of economic units, the legislative, economic and social aspects of the process are scrutiny observed. Some problems of a real example of application of proposed assessment are discussed. Conclusions about methodology and efficiency of different alternatives are made. 2 refs

  10. Surface Restructuring of Hybrid Perovskite Crystals

    KAUST Repository

    Banavoth, Murali

    2016-11-07

    Hybrid perovskite crystals have emerged as an important class of semiconductors because of their remarkable performance in optoelectronics devices. The interface structure and chemistry of these crystals are key determinants of the device\\'s performance. Unfortunately, little is known about the intrinsic properties of the surfaces of perovskite materials because extrinsic effects, such as complex microstructures, processing conditions, and hydration under ambient conditions, are thought to cause resistive losses and high leakage current in solar cells. We reveal the intrinsic structural and optoelectronic properties of both pristinely cleaved and aged surfaces of single crystals. We identify surface restructuring on the aged surfaces (visualized on the atomic-scale by scanning tunneling microscopy) that lead to compositional and optical bandgap changes as well as degradation of carrier dynamics, photocurrent, and solar cell device performance. The insights reported herein clarify the key variables involved in the performance of perovskite-based solar cells and fabrication of high-quality surface single crystals, thus paving the way toward their future exploitation in highly efficient solar cells.

  11. Mergers, Acquisitions and Restructuring: Types, Regulation, and Patterns of Practice

    OpenAIRE

    Coates, John C

    2014-01-01

    An important component of corporate governance is the regulation of significant transactions – mergers, acquisitions, and restructuring. This paper (a chapter in Oxford Handbook on Corporate Law and Governance, forthcoming) reviews how M&A and restructuring are regulated by corporate and securities law, listing standards, antitrust and foreign investment law, and industry-specific regulation. Drawing on real-world examples from the world’s two largest M&A markets (the US and the UK) and a rep...

  12. Competencies of engineering staff in steelworks after their restructuring

    Directory of Open Access Journals (Sweden)

    K. SzczepańsKa-Woszczyna

    2016-04-01

    Full Text Available In the article changes in competencies of engineering staff were presented. The key element of the competencies is metallurgical knowledge. After restructuring process the scale of competencies was widened. Particular changes after restructuring were characterized. Competencies were identified from a broad range of literature and then rated by 101 respondents, managers in terms of importance to jobs of engineers The background for the case study was Human Resources data from ArcelorMittal, Poland.

  13. The effects of corporate restructuring on hospital policymaking.

    Science.gov (United States)

    Alexander, J A; Morlock, L L; Gifford, B D

    1988-06-01

    Hospital corporate restructuring is the segmentation of assets or functions of the hospital into separate corporations. While these functions are almost always legally separated from the hospital, their impact on hospital policymaking may be far more direct. This study examines the effects of corporate restructuring by community hospitals on the structure, composition, and activity of hospital governing boards. In general, we expect that the policymaking function of the hospital will change to adapt to the multicorporate structure implemented under corporate restructuring, as well as the overlapping boards and diversified business responsibilities of the new corporate entity. Specifically, we hypothesize that the hospital board under corporate restructuring will conform more to the "corporate" model found in the business/industrial sector and less to the "philanthropic" model common to most community hospitals to date. Analysis of survey data from 1,037 hospitals undergoing corporate restructuring from 1979-1985 and a comparison group of 1,883 noncorporately restructured hospitals suggests general support for this hypothesis. Implications for health care governance and research are discussed.

  14. Elaboration and acceptability of restructured hams added with jabuticaba skin

    Directory of Open Access Journals (Sweden)

    Ana Paula de Carvalho ALVES

    Full Text Available Abstract Jabuticaba skin flour (JSF, rich in antioxidants and fibre, was used in order to develop four restructured hams in different combinations with meat: 0% control – without the addition of JSF, with the addition of 0.5% JSF, 1.0% JSF and 1.5% JSF, meeting, therefore, the growing demand for meat products with functional appeal. Physicochemical characterizations and sensory analysis were performed, evaluating the impact of information on possible health benefits and the effect on storage. Restructured hams with JSF had higher contents of phenolic compounds; greater weight loss; a darker shade; texture profile with smaller parameters of stiffness, cohesiveness, adhesiveness, flexibility and chewiness (except the restructured ham with 0.5% JSF. Phenolic compounds, pH values, colour and TBARs did not differ with storage time, but were statistically different among JSF concentrations. Panellists preferred the restructured ham without JSF, however, the analysis of variance for overall aspect did not show a significant difference between the restructured ham “control” and those added with 0.5% and 1.0% JSF, after informing the panellists that they would possibly bring health benefits. It is concluded that it is possible to prepare and store restructured hams with JSF, at concentrations of 0.5% and 1.0% with good acceptance.

  15. The effects of corporate restructuring on hospital policymaking.

    Science.gov (United States)

    Alexander, J A; Morlock, L L; Gifford, B D

    1988-01-01

    Hospital corporate restructuring is the segmentation of assets or functions of the hospital into separate corporations. While these functions are almost always legally separated from the hospital, their impact on hospital policymaking may be far more direct. This study examines the effects of corporate restructuring by community hospitals on the structure, composition, and activity of hospital governing boards. In general, we expect that the policymaking function of the hospital will change to adapt to the multicorporate structure implemented under corporate restructuring, as well as the overlapping boards and diversified business responsibilities of the new corporate entity. Specifically, we hypothesize that the hospital board under corporate restructuring will conform more to the "corporate" model found in the business/industrial sector and less to the "philanthropic" model common to most community hospitals to date. Analysis of survey data from 1,037 hospitals undergoing corporate restructuring from 1979-1985 and a comparison group of 1,883 noncorporately restructured hospitals suggests general support for this hypothesis. Implications for health care governance and research are discussed. PMID:3384671

  16. Glycolytic enzyme activities and gene expression in Cicer arietinum exposed to water-deficit stress.

    Science.gov (United States)

    Khanna, Suruchi M; Taxak, Pooja Choudhary; Jain, Pradeep K; Saini, Raman; Srinivasan, R

    2014-08-01

    The specific activities and transcript levels of glycolytic enzymes were examined in shoots of chickpea (Cicer arietinum L.) cultivars, Pusa362 (drought tolerant) and SBD377 (drought sensitive), subjected to water-deficit stress 30 days after sowing. Water-deficit stress resulted in decrease in relative water content, chlorophyll content, plant dry weight, and NADP/NADPH ratio and increase in NAD/NADH ratio in both the cultivars. A successive decline in the specific activities of fructose-1,6-bisphosphate aldolase (aldolase), 3-phosphoglycerate kinase (PGK), and NADP-glyceraldehyde-3-phosphate dehydrogenase (NADP-GAPDH) and elevation in the specific activities of phosphoglycerate mutase (PGM) and triosephosphate isomerase (TPI) was observed in both the cultivars under stress as compared to their respective control plants. The specific activities of hexokinase, fructose-6-phosphate kinase (PFK), and NAD-GAPDH were least affected. The transcript levels of PGK and NADP-GAPDH decreased and that of glucose-6-phosphate isomerase (GPI), PGM, and PFK increased in response to water-deficit stress while water-deficit stress had no effect on the steady-state transcript levels of hexokinase, aldolase, TPI, and NAD-GAPDH. The results suggest that under water-deficit stress, the activities and transcript levels of most of the glycolytic enzymes are not significantly affected, except the increased activity and transcript level of PGM and decreased activities and transcript levels of PGK and NADP-GAPDH. Further, the glycolytic enzymes do not show much variation between the tolerant and sensitive cultivars under water deficit.

  17. Tin-Containing Silicates: Identification of a Glycolytic Pathway via 3-Deoxyglucosone

    DEFF Research Database (Denmark)

    Tolborg, Søren; Meier, Sebastian; Sádaba, I.

    2016-01-01

    a cascade of four to five sequential steps. Currently, there is a limited understanding of the competing glycolytic pathways within these systems. Here we identify dehydration of glucose to 3-deoxyglucosone as an important pathway that occurs in addition to retro-aldol reaction of hexoses when using tin......-containing silicates. It is possible to influence the relative carbon flux through these pathways by controlling the amount of alkali metal salts present in the reaction mixture. In the absence of added potassium carbonate, at least 15–30% carbon flux via 3-deoxyglucosone is observed. Addition of just a few ppm...

  18. Restructuring the Schoolbook Provision System in Indonesia

    Directory of Open Access Journals (Sweden)

    Dedi Supriadi

    1999-03-01

    Full Text Available Schoolbooks account for between 65% and 75% of all publishing activity in Indonesia. The amount of money allocated is continually increasing. Priority is given to the primary and junior secondary school levels (6+3 years, which are compulsory. Between 1969 and 1988, the Government of Indonesia (GOI has produced some 550 million primary textbooks and library books. Up to the year 2000, the GOI has decided to allocate the total of US$ 355.2 million to produce 250 million copies of primary and junior secondary school textbooks to reach the ratio of one book for each student. In addition, around Rp 20-50 billion (US$ 10-20 million annually is spent to purchase 8-17 million copies of reading books which are aimed at stimulating the reading interest of primary school children. These books are distributed free to some 168,000 primary and 26,969 junior secondary schools throughout Indonesia. Following the massive efforts to increase book availability at schools, some innovative policies are being taken. Book evaluation standards have been improved to ensure that only high quality books are used at schools. The distribution system has been restructured to guarantee that books reach targeted schools. Consequently, the book monitoring system has had to be strengthened to examine whether or not the books really reach the schools and are used properly by students and teachers in the classrooms. In the last three years, there has also been a growing concern with multicultural issues in schoolbook provision programs. In such a culturally diverse nation as Indonesia, schoolbooks should also be culturally sensitive and be recognize the varied sociocultural backgrounds which affect students' learning.

  19. Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS.

    Science.gov (United States)

    Mukai, Chinatsu; Gao, Lizeng; Bergkvist, Magnus; Nelson, Jacquelyn L; Hinchman, Meleana M; Travis, Alexander J

    2013-01-01

    Maintaining activity of enzymes tethered to solid interfaces remains a major challenge in developing hybrid organic-inorganic devices. In nature, mammalian spermatozoa have overcome this design challenge by having glycolytic enzymes with specialized targeting domains that enable them to function while tethered to a cytoskeletal element. As a step toward designing a hybrid organic-inorganic ATP-generating system, we implemented a biomimetic site-specific immobilization strategy to tether two glycolytic enzymes representing different functional enzyme families: triose phosphoisomerase (TPI; an isomerase) and glyceraldehyde 3-phosphate dehydrogenase (GAPDHS; an oxidoreductase). We then evaluated the activities of these enzymes in comparison to when they were tethered via classical carboxyl-amine crosslinking. Both enzymes show similar surface binding regardless of immobilization method. Remarkably, specific activities for both enzymes were significantly higher when tethered using the biomimetic, site-specific immobilization approach. Using this biomimetic approach, we tethered both enzymes to a single surface and demonstrated their function in series in both forward and reverse directions. Again, the activities in series were significantly higher in both directions when the enzymes were coupled using this biomimetic approach versus carboxyl-amine binding. Our results suggest that biomimetic, site-specific immobilization can provide important functional advantages over chemically specific, but non-oriented attachment, an important strategic insight given the growing interest in recapitulating entire biological pathways on hybrid organic-inorganic devices.

  20. Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS.

    Directory of Open Access Journals (Sweden)

    Chinatsu Mukai

    Full Text Available Maintaining activity of enzymes tethered to solid interfaces remains a major challenge in developing hybrid organic-inorganic devices. In nature, mammalian spermatozoa have overcome this design challenge by having glycolytic enzymes with specialized targeting domains that enable them to function while tethered to a cytoskeletal element. As a step toward designing a hybrid organic-inorganic ATP-generating system, we implemented a biomimetic site-specific immobilization strategy to tether two glycolytic enzymes representing different functional enzyme families: triose phosphoisomerase (TPI; an isomerase and glyceraldehyde 3-phosphate dehydrogenase (GAPDHS; an oxidoreductase. We then evaluated the activities of these enzymes in comparison to when they were tethered via classical carboxyl-amine crosslinking. Both enzymes show similar surface binding regardless of immobilization method. Remarkably, specific activities for both enzymes were significantly higher when tethered using the biomimetic, site-specific immobilization approach. Using this biomimetic approach, we tethered both enzymes to a single surface and demonstrated their function in series in both forward and reverse directions. Again, the activities in series were significantly higher in both directions when the enzymes were coupled using this biomimetic approach versus carboxyl-amine binding. Our results suggest that biomimetic, site-specific immobilization can provide important functional advantages over chemically specific, but non-oriented attachment, an important strategic insight given the growing interest in recapitulating entire biological pathways on hybrid organic-inorganic devices.

  1. Effect of Intermittent Training on Oxidative and Glycolytic Capacity in Rat Skeletal Muscles

    Directory of Open Access Journals (Sweden)

    A Arabmomeni

    2014-12-01

    Full Text Available Introduction: Any type of exercise protocol has specific effects on the physiology of the body. Thus, according to the purpose of the training program and conditions of the subjects, a specific exercise protocol is needed to be considered. Therefore, the purpose of this study was to assess the effect of intermittent training on oxidative and glycolytic capacity in rat skeletal muscles. Methods: Forty male rats were divided into two old (27 mon, 389±31 g and young groups (3 ± 4 mon, 224±14 g, each of which were randomly divided into control and experimental groups (n = 10. Training group performed intermittent exercise on a treadmill 6 times /week for 8weeks. It consisted of 10 bouts of 4 min running interspersed by 2 min of active rest. All rats were anesthetized, 24 hours after the last session exercise, and Soleus (SOL and extensor digitorumlongus (EDL muscles were removed rapidly. In fact, the tissues were analyzed in regard with CS and LDH enzymes activities. In order to analyze the study data, one way-ANOVA and Tukey's post-hoc tests were applied. Results: The results demonstrated that CS enzyme activity in EDL and SOL muscles increased significantly in both, old (OT and young groups (YT (p 0.05. Conclusion: The results suggested that the training method described in the present study can be quite beneficial to the young and old, when a simultaneous increase in oxidative and glycolytic capacity is aimed.

  2. Glycogen Shunt Activity and Glycolytic Supercompensation in Astrocytes May Be Distinctly Mediated via the Muscle Form of Glycogen Phosphorylase

    DEFF Research Database (Denmark)

    Jakobsen, Emil; Bak, Lasse K; Walls, Anne B

    2017-01-01

    Glycogen is the main storage form of glucose in the brain. In contrast with previous beliefs, brain glycogen has recently been shown to play important roles in several brain functions. A fraction of metabolized glucose molecules are being shunted through glycogen before reentering the glycolytic...... pathway, a phenomenon known as the glycogen shunt. The significance of glycogen in astrocyte energetics is underlined by high activity of the glycogen shunt and the finding that inhibition of glycogen degradation, under some conditions leads to a disproportional increase in glycolytic activity, so......-called glycolytic supercompensation. Glycogen phosphorylase, the key enzyme in glycogen degradation, is expressed in two different isoforms in brain, the muscle and the brain isoform. Recent studies have illustrated how these are differently regulated. In the present study, we investigate the role of the two...

  3. The effects of union mergers and internal restructuring:

    DEFF Research Database (Denmark)

    Navrbjerg, Steen Erik; Larsen, Trine Pernille

    2016-01-01

    Trade union mergers and restructuring are often seen as strategy for union revitalisation and renewal and have been ongoing in the majority of European trade unions. This paper explores how recent union mergers and internal restructuring has affected unions service provision, union democracy...... and interest representation. This is analysed drawing on longitudinal data from two Danish shop steward surveys conducted in 1998 and 2010, comparing shop stewards in merged and non-merged unions respectively. The main results are that although it could be expected that union mergers and internal restructuring...... may entail lower service levels, democratic deficit and weakening of union identities, this is not always the case. Danish shop stewards appear more satisfied with their union’s service provision anno 2010 compared to 1998 and feel more able to influence union politics. However, variations also exist...

  4. Designing incentive market mechanisms for improving restructured power system reliabilities

    DEFF Research Database (Denmark)

    Ding, Yi; Østergaard, Jacob; Wu, Qiuwei

    2011-01-01

    In a restructured power system, the monopoly generation utility is replaced by different electricity producers. There exists extreme price volatility caused by random failures by generation or/and transmission systems. In these cases, producers' profits can be much higher than those in the normal...... state. The reliability management of producers usually cannot be directly controlled by the system operators in a restructured power system. Producers may have no motivation to improve their reliabilities, which can result in serious system unreliability issues in the new environment. Incentive market...... mechanisms for improving the restructured power system reliabilities have been designed in this paper. In the proposed incentive mechanisms, penalty will be implemented on a producer if the failures of its generator(s) result in the variation of electricity prices. Incentive market mechanisms can motivate...

  5. JURIDICAL AND FISCAL ASPECTS REGARDING THE RESTRUCTURING OF

    Directory of Open Access Journals (Sweden)

    DRAGOS (COMAN DORICA

    2015-08-01

    Full Text Available The purpose of this paper is the study of the juridical and fiscal aspects regarding the restructuring of the economical entities. Based on analyzing of the different materials on legislative advancements at national level and on European directives, rules and recommendations and other reference works, this study accomplished a synthesis of the main juridical aspects regarding the economical restructuring in Romania and in the European Union. The study emphasizes the importance of the knowledge of the fiscal aspects in order to draft a fiscal strategy ,which needs to be developed prior to any formal offers or detailed studying of the target company, and which reveals the characteristics, the stages and the effects of the fusion operation of an economic entity. The results of the study points out the main juridical and fiscal aspects regarding the restructuring of the trading companies, as well as the main components of a fusion operation.

  6. COGNITIVE RESTRUCTURING: ALTERNATIVE COUNSELING TECHNIQUES TO REDUCTION ACADEMIC PROCRASTINATION

    Directory of Open Access Journals (Sweden)

    Annisa Sofiana

    2016-12-01

    Full Text Available Procrastination is often experienced by almost everyone, including students who often delay to resolve any responsibility in the academic process that would decrease the individual academic achievement. cognitive restructuring is one of the cognitive techniques used in counseling in addition to cognitive behavioral techniques (behavioral and didaktif techniques. This technique has several procedures by focusing on identifying an effort and changing dysfunctional thoughts or negative self-statements into a new belief that is more rational and adaptive, which will affect more rational behavior anyway. Cognitive restructuring techniques assessed to be an alternative counseling techniques in reducing academic procrastination.

  7. Consequences of electricity restructuring on the environment: A survey

    International Nuclear Information System (INIS)

    Sevi, Benoit

    2004-01-01

    The aim of this paper is to assess theoretical consequences of restructuring electricity markets on the environment. We examine changes in potential behaviours in consumption-side as well as in supply-side. We show that restructuring and following access to competition is not neutral from an environmental standpoint. Deregulation could induce some negative externalities due to requirements in cost-efficiency. The principal result of this paper is the need of strong incentives in public policies to compensate the new short-term horizon in which energy sector's firms are evolving, particularly concerning R and D. (author)

  8. Syllable restructuring in early Solomon Islands Pidgin English

    Directory of Open Access Journals (Sweden)

    Andrei A. Avram

    2007-01-01

    Full Text Available The present paper looks at the various syllable restructuring strategies used in early Solomon Islands Pidgin English. These depend on the phonological shape of the etyma and consist of epenthesis, paragoge and consonant deletion. Also examined is the quality of the epenthetic and of the paragogic vowels. Contrary to claims recently put forth in the literature, vowel harmony appears to play no part in the selection of these intrusive vowels. Finally, syllable restructuring in early Solomon Islands Pidgin English is shown to have striking similarities to that attested in other early varieties of Melanesian Pidgin English.

  9. Global lessons from restructuring : what works, what doesn't : words from away

    International Nuclear Information System (INIS)

    Littlechild, S.

    2002-01-01

    This paper reviewed some basic lessons learned about restructuring within the electric power industry with particular reference to network regulations, competition in generation, competition in retail supply, wholesale trading, performance in the United Kingdom, and European Union policy. It appears that ownership separation of the transmission grid is a component of restructuring that actually works, as does duty on transmission, the legal and management separation of distribution networks from generation and retail supply. Fully integrated utilities do not work. Neither does accounting separation alone. In terms of network regulation, incentive price caps work, but letting companies decide cost allocations does not. In order for generation competition to work, there must be many generators. In order for retail competition to work, markets must be opened to large and medium customers and retail price controls must be removed. It was noted that the approach taken in California does not work, including retail price caps below generation prices, and preventing utilities from signing hedging contracts. In wholesale trading, published prices and open markets work to a certain degree. However, wholesale trading arrangements cannot work if there is no demand side. Independent sector regulation appears to work, but no sector regulation in the early stages such as in New Zealand and Germany does not. In the United Kingdom, prices have come down 25 to 35 per cent since 1990. Annual network investment is 50 per cent higher, and complaints have been cut in half. All customers have a choice. The European Union Policy is to speed up liberalisation. In March 2001, a directive was proposed in which all consumers be given the freedom to choose a supplier for non residential use by January 1, 2003 for electricity, and January 1, 2004 for gas and for residential use by January 1, 2005

  10. Potential role of the glycolytic oscillator in acute hypoxia in tumors

    Science.gov (United States)

    Che Fru, Leonard; Adamson, Erin B.; Campos, David D.; Fain, Sean B.; Jacques, Steven L.; van der Kogel, Albert J.; Nickel, Kwang P.; Song, Chihwa; Kimple, Randall J.; Kissick, Michael W.

    2015-12-01

    Tumor acute hypoxia has a dynamic component that is also, at least partially, coherent. Using blood oxygen level dependent magnetic resonance imaging, we observed coherent oscillations in hemoglobin saturation dynamics in cell line xenograft models of head and neck squamous cell carcinoma. We posit a well-established biochemical nonlinear oscillatory mechanism called the glycolytic oscillator as a potential cause of the coherent oscillations in tumors. These data suggest that metabolic changes within individual tumor cells may affect the local tumor microenvironment including oxygen availability and therefore radiosensitivity. These individual cells can synchronize the oscillations in patches of similar intermediate glucose levels. These alterations have potentially important implications for radiation therapy and are a potential target for optimizing the cancer response to radiation.

  11. Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

    DEFF Research Database (Denmark)

    Jacobs, Robert A; Díaz, Víctor; Soldini, Lavinia

    2013-01-01

    -resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks......) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085......) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type....

  12. Impaired metabolism of senescent muscle satellite cells is associated with oxidative modifications of glycolytic enzymes

    DEFF Research Database (Denmark)

    Baraibar, Martin; Hyzewicz, Janek; Rogowska-Wrzesinska, Adelina

    2014-01-01

    lipids for energy production. Such changes reflect alterations in membrane composition and dysregulation of sphingolipids signaling during senescence. This study establishes a new concept connecting oxidative protein modifications with the altered cellular metabolism associated with the senescent...... is assured by resident adult stem cells known as satellite cells. During senescence their replication and differentiation is compromised contributing to sarcopenia. In this study we have addressed the impact of oxidatively modified proteins in the impaired metabolism of senescent human satellite cells....... By using a targeted proteomics analysis we have found that proteins involved in protein quality control and glycolytic enzymes are the main targets of oxidation (carbonylation) and modification with advanced glycation/lipid peroxidation end products during replicative senescence of satellite cells...

  13. Portuguese propolis disturbs glycolytic metabolism of human colorectal cancer in vitro

    Science.gov (United States)

    2013-01-01

    Background Propolis is a resin collected by bees from plant buds and exudates, which is further processed through the activity of bee enzymes. Propolis has been shown to possess many biological and pharmacological properties, such as antimicrobial, antioxidant, immunostimulant and antitumor activities. Due to this bioactivity profile, this resin can become an alternative, economic and safe source of natural bioactive compounds. Antitumor action has been reported in vitro and in vivo for propolis extracts or its isolated compounds; however, Portuguese propolis has been little explored. The aim of this work was to evaluate the in vitro antitumor activity of Portuguese propolis on the human colon carcinoma cell line HCT-15, assessing the effect of different fractions (hexane, chloroform and ethanol residual) of a propolis ethanol extract on cell viability, proliferation, metabolism and death. Methods Propolis from Angra do Heroísmo (Azores) was extracted with ethanol and sequentially fractionated in solvents with increasing polarity, n-hexane and chloroform. To assess cell viability, cell proliferation and cell death, Sulforhodamine B, BrDU incorporation assay and Anexin V/Propidium iodide were used, respectively. Glycolytic metabolism was estimated using specific kits. Results All propolis samples exhibited a cytotoxic effect against tumor cells, in a dose- and time-dependent way. Chloroform fraction, the most enriched in phenolic compounds, appears to be the most active, both in terms of inhibition of viability and cell death. Data also show that this cytotoxicity involves disturbance in tumor cell glycolytic metabolism, seen by a decrease in glucose consumption and lactate production. Conclusion Our results show that Portuguese propolis from Angra do Heroísmo (Azores) can be a potential therapeutic agent against human colorectal cancer. PMID:23870175

  14. The Phosphoglucose Isomerase from the Hyperthermophilic Archaeon Pyrococcus furiosus is a Unique Glycolytic Enzyme that belongs to the Cupin Superfamily

    NARCIS (Netherlands)

    Verhees, C.H.; Huynen, M.A.; Ward, D.E.; Schiltz, E.; Vos, de W.M.; Oost, van der J.

    2001-01-01

    Pyrococcus furiosus uses a variant of the Embden-Meyerhof pathway during growth on sugars. All but one of the genes that encode the glycolytic enzymes of P. furiosus have previously been identified, either by homology searching of its genome or by reversed genetics. We here report the isolation of

  15. Frequent and recent retrotransposition of orthologous genes plays a role in the evolution of sperm glycolytic enzymes

    Directory of Open Access Journals (Sweden)

    de Villena Fernando

    2010-05-01

    Full Text Available Abstract Background The central metabolic pathway of glycolysis converts glucose to pyruvate, with the net production of 2 ATP and 2 NADH per glucose molecule. Each of the ten reactions in this pathway is typically catalyzed by multiple isozymes encoded by a multigene family. Several isozymes in this pathway are expressed only during spermatogenesis, and gene targeting studies indicate that they are essential for sperm function and male fertility in mouse. At least three of the novel glycolytic isozymes are encoded by retrogenes (Pgk2, Aldoart1, and Aldoart2. Their restricted expression profile suggests that retrotransposition may play a significant role in the evolution of sperm glycolytic enzymes. Results We conducted a comprehensive genomic analysis of glycolytic enzymes in the human and mouse genomes and identified several intronless copies for all enzymes in the pathway, except Pfk. Within each gene family, a single orthologous gene was typically retrotransposed frequently and independently in both species. Several retroposed sequences maintained open reading frames (ORFs and/or provided evidence of alternatively spliced exons. We analyzed expression of sequences with ORFs and Gpi1 transcript in mouse spermatogenic cells. Conclusions Our analysis detected frequent, recent, and lineage-specific retrotransposition of orthologous glycolytic enzymes in the human and mouse genomes. Retrotransposition events are associated with LINE/LTR and genomic integration is random. We found evidence for the alternative splicing of parent genes. Many retroposed sequences have maintained ORFs, suggesting a functional role for these genes.

  16. Adenosine triphosphate levels during anaphylactic histamine release in rat mast cells in vitro. Effects of glycolytic and respiratory inhibitors

    DEFF Research Database (Denmark)

    Johansen, Torben

    1979-01-01

    The adenosine triphosphate (ATP) content of rat mast cells was studied during and after anaphylactic histamine release. The almost identical time course of ATP decrease from mast cells treated with either glycolytic or respiratory inhibitors supports the view that the ATP depletion was largely re...

  17. Restructuring innovation systems in India through migration

    OpenAIRE

    Khadria, B.; Meyer, Jean-Baptiste

    2013-01-01

    The paper discusses the role of migration in shaping the innovation systems in affected countries. The first section would clarify how the stereotypical benefits of high skill migration are nullified by three dynamic conflicts of interest between the source and the destination countries viz. age, wage and vintage. It would also argue that the relationship between diaspora knowledge networks and development in the home countries is a complex one. The second section would explain the divergence...

  18. Agency Theory: Its Relevance to Tanzania's Economic Restructuring ...

    African Journals Online (AJOL)

    The relevance of the theory to the restructuring programme is also examined. The paper analyses how privatization is going to define the agency relationships, thereby enabling the application to agency theory in the operations of these companies. This will mutually benefit both investors and management in these ...

  19. The effect of restructuring of health care services on caesarean ...

    African Journals Online (AJOL)

    Objective. To review the anticipated changes in caesarean section (CS) rates following the restructuring of maternity health care services from regional to district level. Hypothesis. A change in provision of maternity services from regional to district level results in a decline in the CS rates. Method. A retrospective audit was ...

  20. Revenue and Health Impacts of Restructuring Tobacco Excise Tax ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Revenue and Health Impacts of Restructuring Tobacco Excise Tax in the Philippines. A proposed law in the Philippines to increase the excise tax on tobacco by 215% will likely have implications for tobacco control and consumption, and public health, not just for that country but for the region. Although half of deaths due to ...

  1. Restructuring STM (Science, Technology, and Mathematics) Education for Entrepreneurship

    Science.gov (United States)

    Ezeudu, F. O.; Ofoegbu, T. O.; Anyaegbunnam, N. J.

    2013-01-01

    This paper discussed the need to restructure STM (science, technology, and mathematics) education to reflect entrepreneurship. This is because the present STM education has not achieved its aim of making graduates self-reliant. Entrepreneurship education if introduced in the STM education will produce graduate who can effectively manage their…

  2. The White Paper and Restructuring the Academic Labour Market.

    Science.gov (United States)

    Barlow, Kerry

    1989-01-01

    Mechanisms that allow the strengthening of market and patriarchal forces in higher education, especially as they relate to the academic labor market, are illustrated. The Dawkins paper, "Higher Education, a Policy Statement" (the White Paper), represents an attempt to implement major restructuring in Australia. (MLW)

  3. Effectiveness of cognitive restructuring and social decision-making ...

    African Journals Online (AJOL)

    This study investigated effectiveness of cognitive restructuring and social decision making techniques on truancy reduction among secondary school adolescents in Ibadan, Nigeria. Pre-test, post-test, control group quasiexperimental design with a 3x2x2 factorial matrix was adopted for the study. A total of 180 Senior ...

  4. An evaluation of the proposed organisation restructuring at Kadoma ...

    African Journals Online (AJOL)

    Introduction: Restructuring is the corporate management term for the act of reorganizing the legal, operational, or other structures of a company for the purpose of making it more profitable or better organized for its present needs. However, preparing an organization to accept and welcome any change is crucial. There is ...

  5. Accounting for change: the micropolitics of university restructuring ...

    African Journals Online (AJOL)

    In this third and final article on "the micropolitics of university restructuring", the links are drawn between globalization pressures on the third world state and the ways in which higher education institutions are affected in the same ways by the state and globalization, given the very different histories, capacities and ideologies ...

  6. The effect of restructuring of health care services on caesarean ...

    African Journals Online (AJOL)

    Nicky

    2005-06-24

    Jun 24, 2005 ... The effect of restructuring of health care services on caesarean section rates. ARTICLE. Medical Research Council/ University of KwaZulu-Natal, Pregnancy Hypertension Research. Unit and Department of Obstetrics and Gynaecology, Nelson R Mandela School of Health. Sciences, University of ...

  7. Business restructuring and the strategic role of 'IT'

    International Nuclear Information System (INIS)

    Lindsay, E.

    1998-01-01

    Westcoast Energy has recently undergone a major restructuring as a result of industry deregulation. As a result of the restructuring in 1997, Westcoast, producers, shippers and customers renegotiated gathering and business processes and devised a new tolling methodology known as the Negotiated Toll Settlement. This paper describes the new structure of Westcoast, the lines of business the company is engaged in, the nature of the Negotiated Toll Settlement with emphasis on the important role that information technology (IT) played in the company's business restructuring strategies. In discussing the impact of restructuring and the Negotiated Toll Settlement on information technology, attention is drawn to the enormity of the cultural change faced by employees largely unfamiliar with change. It discusses the new skills and competencies required and the complexity introduced by the need for timeliness. The difficulties of having the delivery team in Vancouver and system support and customers in Calgary and the massive changes that had to be made to refocus attention on customer and operational concerns are outlined. Among the lessons learned, cultural change was identified as perhaps the most difficult stumbling block, followed by the need for communication, quality assurance, risk assessment and project management methodologies, and recognition of the direct relationship between business and system changes. figs

  8. Army Drawdown and Restructuring: Background and Issues for Congress

    Science.gov (United States)

    2014-02-28

    as “worn-out leftovers from World War II.” The low personnel and equipment readiness levels in Army Drawdown and Restructuring: Background and...grew to over 1,570,000 men and women . The conscripted Army of the Vietnam War had a disproportionate representation of lower-income and non- college

  9. Accounting for change: the micropolitics of university restructuring ...

    African Journals Online (AJOL)

    This second article in a three-part series published in the South African Journal of Higher Education, describes the various ways in which "academic identity" informed the politics and shaped the outcomes of "restructuring" at the University of Durban Westville (UDW) in the late 1990s. The authors argue that while ...

  10. Strategies for improving job security: decreasing anxiety from restructuring.

    Science.gov (United States)

    Gilmore, A

    1995-12-01

    Nurses are worried about the future of nursing and, more specifically, about the future of their own job. This author who is experienced in working with nurses on job security issues and clarifying layoff policies suggests strategies that can help improve nurses' feelings of job security even during this time of restructuring.

  11. Genomic restructuring in F1 Hordeum chilense × durum wheat ...

    Indian Academy of Sciences (India)

    Genomic restructuring in F1 Hordeum chilense × durum wheat hybrids and corresponding hexaploid tritordeum lines revealed by DNA fingerprinting analyses. ANDREIA DELGADO ANA CARVALHO AZAHARA CARMEN MARTÍN ANTONIO MARTÍN JOSÉ EDUARDO LIMA-BRITO. Online Resource Volume 96 Online ...

  12. The Study of the Effects of Cognitive Restructuring Therapy on ...

    African Journals Online (AJOL)

    This paper is an extract from a larger study. The study investigated the relative effectiveness of Cognitive Restructuring (C.R) therapy in the reduction of cigarette smoking behaviour of undergraduate students from South West of Nigeria. The research followed a pre test post test control group design. sixty volunteer ...

  13. Challenges of electric power industry restructuring for fuel suppliers

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-09-01

    The purpose of this report is to provide an assessment of the changes in other energy industries that could occur as the result of restructuring in the electric power industry. This report is prepared for a wide audience, including Congress, Federal and State agencies, the electric power industry, and the general public. 28 figs., 25 tabs.

  14. Restructuring, Teams, and Learning: The Case of a Clothing Company.

    Science.gov (United States)

    Brown, Tony

    1999-01-01

    Interviews with female employees of a clothing company moving to lean production and teamwork indicate that Taylorism is being reinvented in the contemporary workplace. Restructuring produces higher productivity but greater work pressures and lower wages, despite rhetoric about autonomy, job satisfaction, and workplace democracy. (Contains 57…

  15. Public-policy responsibilities in a restructured electricity industry

    Energy Technology Data Exchange (ETDEWEB)

    Tonn, B.; Hirst, E.; Bauer, D.

    1995-06-01

    In this report, we identify and define the key public-policy values, objectives, and actions that the US electricity industry currently meets. We also discuss the opportunities for meeting these objectives in a restructured industry that relies primarily on market forces rather than on government mandates. And we discuss those functions that governments might undertake, presumably because they will not be fully met by a restructured industry on its own. These discussions are based on a variety of inputs. The most important inputs came from participants in an April 1995 workshop on Public-Policy Responsibilities and Electric Industry Restructuring: Shaping the Research Agenda. Other sources of information and insights include the reviews of a draft of this report by workshop participants and others and the rapidly growing literature on electric-industry restructuring and its implications. One of the major concerns about the future of the electricity industry is the fate of numerous social and environmental programs supported by today`s electric utilities. Many people worry that a market-driven industry may not meet the public-policy objectives that electric utilities have met in the past. Examples of potentially at-risk programs include demand-side management (DSM), renewable energy, low-income weatherization, and fuel diversity. Workshop participants represented electric utilities, public utility commissions (PUCs), state energy offices, public-interest groups, other energy providers, and the research community.

  16. Guest Editorial: Globalisation and higher education restructuring in ...

    African Journals Online (AJOL)

    Guest Editorial: Globalisation and higher education restructuring in South Africa: moving towards distributive justice. Y Waghid, L le Grange. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT · http://dx.doi.org/10.4314/sajhe.v16i1.25265 · AJOL African Journals Online.

  17. Challenges of electric power industry restructuring for fuel suppliers

    International Nuclear Information System (INIS)

    1998-09-01

    The purpose of this report is to provide an assessment of the changes in other energy industries that could occur as the result of restructuring in the electric power industry. This report is prepared for a wide audience, including Congress, Federal and State agencies, the electric power industry, and the general public. 28 figs., 25 tabs

  18. Load frequency control of an asynchronous restructured power system

    African Journals Online (AJOL)

    This paper presents the analysis of load frequency control (LFC) of a two-area restructured power system interconnected via parallel ac/dc transmission links. Simulation results show that the limitations of PI controller can be overcome by including Fuzzy logic concept and thereby the dynamic performance can be improved ...

  19. Assessing The Impact Of Railway Restructuring In Nigeria | Ibe ...

    African Journals Online (AJOL)

    Benchmarking method was used to determine the best restructuring model for Nigeria. Descriptive statistical tools and simulation model were also used in carrying out the analysis. In this respect, we identified low investment, low technological capacity and capability, low productivity unreliable services, High operating ...

  20. The San Diego Panasonic Partnership: A Case Study in Restructuring.

    Science.gov (United States)

    Holzman, Michael; Tewel, Kenneth J.

    1992-01-01

    The Panasonic Foundation provides resources for restructuring school districts. The article examines its partnership with the San Diego City School District, highlighting four schools that demonstrate promising practices and guiding principles. It describes recent partnership work on systemic issues, noting the next steps to be taken in San Diego.…

  1. Social exclusion, access and the restructuring of higher education in ...

    African Journals Online (AJOL)

    The article presents an overview of positions and perspectives on access by critically analysing the new funding formula, the restructuring of higher education and policy shifts that foreground output and efficiency as main levers of the transformation of higher education in South Africa. South African Journal of Higher ...

  2. Free trade and freer petchems drive Mexican restructuring

    International Nuclear Information System (INIS)

    Wood, A.

    1992-01-01

    When Mexico first opened up its protected markets in 1987 by cutting import tariffs, it thrust the chemical industry into a phase of change. Now, with the advent of the North American Free Trade Agreement(NAFTA) and the liberalization of petrochemicals by state oil group Petroleos Mexicanos (Pemex), restructuring has moved up a gear

  3. Functional pools of oxidative and glycolytic fibers in human muscle observed by 31P magnetic resonance spectroscopy during exercise

    International Nuclear Information System (INIS)

    Park, J.H.; Brown, R.L.; Park, C.R.; McCully, K.; Cohn, M.; Haselgrove, J.; Chance, B.

    1987-01-01

    Quantitative probing of heterogeneous regions in muscle is feasible with phosphorus-31 magnetic resonance spectroscopy because of the differentiation of metabolic patterns of glycolytic and oxidative fibers. A differential recruitment of oxidative and glycolytic fibers during exercise was demonstrated in 4 of 10 untrained young men by following changes in phosphate metabolites. Concentrations of inorganic phosphate (P/sub i/), phosphocreatine, and ATP were estimated in the wrist flexor muscles of the forearm at rest, during two cycles of three grades of exercise, and in recovery. At high work levels (40% of maximum strength), two distinct P/sub i/ peaks were observed and identified with P/sub i/ pools at pH 6.9 and pH 5.9-6.4, respectively. These could be accounted for as follows. At the lowest level of work (using 20% of maximum strength), early recruitment primarily of oxidative (type I) and possibly some intermediate (type IIA) muscle fibers occurs with relatively little net lactate production and consequently little decrease in pH. At higher work loads, however, primarily glycolytic (type IIB) muscle fibers are recruited, which have relatively high net lactate production and therefore generate a second pool of P/sub i/ at low pH. These observations indicated exhaustion of glycolytic type IIB fibers, removal of lactate by high local blood flow, and sustained contractions largely by oxidative type I and IIA fibers. A functional differentiation of fiber types could also be demonstrated during recovery if exercise was stopped while two pools of P/sub i/ were still apparent. The potential of magnetic resonance spectroscopy to characterize oxidative and glycolytic fibers, predict capacity for aerobic performance, and signal the presence of muscle pathology is discussed

  4. School Restructuring: What Works When? A Guide for Education Leaders. Third Edition

    Science.gov (United States)

    Learning Point Associates, 2010

    2010-01-01

    The purpose of this guide is to help chronically struggling schools restructure. "Restructuring" means major, rapid changes that affect how a school is led and how instruction is delivered. Restructuring is essential in achieving rapid improvements in student learning. The focus is on helping education leaders choose strategies that…

  5. Expanding the Evaluation Department Role in a Restructuring District: A Technical Assistance Model.

    Science.gov (United States)

    Borton, William M.

    Although San Diego City Schools is entering its fourth year of restructuring efforts, many schools are still reluctant to assume the autonomy offered to them. If school restructuring is stalling, it is because district offices have not yet restructured, research on what is known about implementing changes has been ignored, and no clear methodology…

  6. Restructuring of the corporate sector of national economy: content and mechanism of realization

    Directory of Open Access Journals (Sweden)

    S. I. Gusakov

    2016-08-01

    It has been suggested to distinguish three directions of the restructuring: inner corporate restructuring; restructuring of relations; economics conditions restructuring. Inner corporate restructuring includes property forms changes, entity structure changes, capital structure changes, form of incorporation changes, management bodies changes, management changes, staff politics changes, reorganization, liquidation, privatization, corporatization. Restructuring of relations includes sales market changes, conversion of enterprises, diversification, contractor’s changes, property assignation, lease, concession, franchising, debt’s restructuring, changes of credit politics, technical supplement, using alternative power sources, standardization etc. Economics conditions restructuring includes regulation and legislation changes, tax regulation changes, currency market changes, changes in credit policies, changers in stock market. Accordingly, every restructuring direction includes many mechanisms of its possible fulfillment. The author has distinguished twenty six mechanisms all the restructuring and they are presented in the table form.The corporate management models’ use in correlation to corporate restructuring models has been described in the article. The author has come to the conclusion that pragmatic model of the corporate restructuring is more preferable in comparison with world recognized valuable model because of the peculiarities of the post-social economy in Ukraine.

  7. The impact of restructuring on employee well-being: a systematic review of longitudinal studies

    NARCIS (Netherlands)

    Jong, T. de; Wiezer, N.; Weerd, M. de; Nielsen, K.; Mattila-Holappa, P.; Mockałłod, Z.

    2016-01-01

    This is a review of published longitudinal empirical research on the impact of restructuring on employee well-being. We investigated whether restructuring accompanied by staff reductions impacts differently on worker well-being than restructuring without staff reductions, and the differences between

  8. Empirical Correlates and Expanded Interpretation of the MMPI-2-RF Restructured Clinical Scale 3 (Cynicism)

    Science.gov (United States)

    Ingram, Paul B.; Kelso, Kristy M.; McCord, David M.

    2011-01-01

    The recent release of the Minnesota Multiphasic Personality Inventory-2-Restructured Form (MMPI-2-RF) has received much attention from the clinical psychology community. Particular concerns have focused on Restructured Clinical Scale 3 (RC3; Cynicism). This article briefly reviews the major criticisms and responses regarding the restructuring of…

  9. Actin restructuring during Salmonella typhimurium infection investigated by confocal and super-resolution microscopy

    Science.gov (United States)

    Han, Jason J.; Kunde, Yuliya A.; Hong-Geller, Elizabeth; Werner, James H.

    2014-01-01

    We have used super-resolution optical microscopy and confocal microscopy to visualize the cytoskeletal restructuring of HeLa cells that accompanies and enables Salmonella typhimurium internalization. Herein, we report the use of confocal microscopy to verify and explore infection conditions that would be compatible with super-resolution optical microscopy, using Alexa-488 labeled phalloidin to stain the actin cytoskeletal network. While it is well known that actin restructuring and cytoskeletal rearrangements often accompany and assist in bacterial infection, most studies have employed conventional diffraction-limited fluorescence microscopy to explore these changes. Here we show that the superior spatial resolution provided by single-molecule localization methods (such as direct stochastic optical reconstruction microscopy) enables more precise visualization of the nanoscale changes in the actin cytoskeleton that accompany bacterial infection. In particular, we found that a thin (100-nm) ring of actin often surrounds an invading bacteria 10 to 20 min postinfection, with this ring being transitory in nature. We estimate that a few hundred monofilaments of actin surround the S. typhimurium in this heretofore unreported bacterial internalization intermediate.

  10. Economic-efficiency considerations in restructuring electric markets

    Energy Technology Data Exchange (ETDEWEB)

    Hill, L.J.

    1996-12-01

    In response to the Energy Policy Act of 1992 and the Federal Energy Regulatory Commission`s subsequent rulemaking on transmission access, many states are exploring options to restructure their electric industries. In their deliberations on restructuring, policymakers should consider (1) the reliability of the electric system; (2) income-distribution effects on ratepayers and utilities; (3) social consequences such as effects on energy conservation, renewable energy, and the environment; and (4) economic efficiency. We address economic-efficiency considerations in this study. Economic efficiency is important because it is one of the primary reasons that policymakers should consider restructuring in the first place: improving the electric-industry`s efficiency lowers costs and, hence, electric prices. In this study, we look at the sources of (in)efficiency in existing and proposed electric markets with the objective of guiding policymakers to design efficient electric markets. The advantages of a competitive market are well known: it leads to lower costs for the utility, lower prices for consumers, more product choices, better customer service, and often the need for less regulation by federal and state agencies. In the short run, firms who cannot produce at the market-clearing price are forced to leave the industry, ensuring that customers have the lowest price possible. In the long run, competition promotes innovation and lower costs. The physical and institutional characteristics of the U.S. electric industry, however, could be impediments to attaining efficiently run, competitive markets. Because of these characteristics, there are multiple sources of efficiencies and inefficiencies in existing electric markets, and there will be multiple sources in restructured ones. The objective of policymakers should not be to trade one set of inefficiencies in existing electric markets for another set in restructured markets.

  11. Economic-efficiency considerations in restructuring electric markets

    International Nuclear Information System (INIS)

    Hill, L.J.

    1996-12-01

    In response to the Energy Policy Act of 1992 and the Federal Energy Regulatory Commission's subsequent rulemaking on transmission access, many states are exploring options to restructure their electric industries. In their deliberations on restructuring, policymakers should consider (1) the reliability of the electric system; (2) income-distribution effects on ratepayers and utilities; (3) social consequences such as effects on energy conservation, renewable energy, and the environment; and (4) economic efficiency. We address economic-efficiency considerations in this study. Economic efficiency is important because it is one of the primary reasons that policymakers should consider restructuring in the first place: improving the electric-industry's efficiency lowers costs and, hence, electric prices. In this study, we look at the sources of (in)efficiency in existing and proposed electric markets with the objective of guiding policymakers to design efficient electric markets. The advantages of a competitive market are well known: it leads to lower costs for the utility, lower prices for consumers, more product choices, better customer service, and often the need for less regulation by federal and state agencies. In the short run, firms who cannot produce at the market-clearing price are forced to leave the industry, ensuring that customers have the lowest price possible. In the long run, competition promotes innovation and lower costs. The physical and institutional characteristics of the U.S. electric industry, however, could be impediments to attaining efficiently run, competitive markets. Because of these characteristics, there are multiple sources of efficiencies and inefficiencies in existing electric markets, and there will be multiple sources in restructured ones. The objective of policymakers should not be to trade one set of inefficiencies in existing electric markets for another set in restructured markets

  12. System development and asset management under restructuring

    International Nuclear Information System (INIS)

    2007-01-01

    In many developed countries the electricity infrastructure contains ageing assets. Because of the need of a continuing operation and higher load factors on the network, the opportunities for replacement or refurbishment are limited. These must be regarded as an opportunity to review the structure of the system and to consider the design and development required to meet the new requirements, taking advantage of new available technologies: HVDC, FACTS, cables, etc. The symposium examines assets management issues and assesses strategies for overhauling existing systems, taking into account the impact on market operation, on supply security and environment

  13. The restructuring of Africa's electricity sector : lessons learned; La restructuration du secteur de l'electricite en Afrique : lecons et retour d'experience

    Energy Technology Data Exchange (ETDEWEB)

    Fall, A. [Commission de Regulation du Secteur de l' Electricite du Senegal (Senegal)

    2008-01-15

    The challenges facing the electricity sector in Africa was discussed with particular reference to financial constraints and the inefficient management of public utilities. The 2 types of reform in the electricity sector include the disengagement of the State for the benefit of the private sector, and the restructuring of the electrical industry to include competition for greater efficiency in the sector. The first reform in Africa involved the opening of the sector to private enterprises without the restructuring of the industry. With the exception of Uganda, the evolution of the restructuring did not include a complete de-integration in the transitional stage. Restructuring involved systematic independent production; a single buyer model with a limited period of monopoly on bulk purchase; access to third party on the networks; empowerment of regulation by the creation of an independent body responsible for regulation; and, establishment of a rural electrification agency. In more advanced countries such as Egypt, Algeria and Nigeria, the creation of a holding company with legally separated subsidiaries was retained. One of the major problems was financial investment, technical performances and the extension of electrical access. Low competition, or low negotiation capabilities led to unbalanced contracts in favour of private promoters. In general, rates for electrical energy increased due to the reform, with the lowering of public resources at the profit of private funding, notably in the independent production of thermal energy versus hydroelectric energy. The results have been mixed due to the breakdown of public versus private partnerships in many countries, with the disengagement of the State. Independent power production has raised problems of sustainability and environmental issues, as well as social problems, price control, as well as accessibility in rural settings. 7 refs., 1 fig.

  14. A Petiveria alliacea standardized fraction induces breast adenocarcinoma cell death by modulating glycolytic metabolism.

    Science.gov (United States)

    Hernández, John Fredy; Urueña, Claudia Patricia; Cifuentes, Maria Claudia; Sandoval, Tito Alejandro; Pombo, Luis Miguel; Castañeda, Diana; Asea, Alexzander; Fiorentino, Susana

    2014-05-14

    Folk medicine uses aqueous and alcoholic extracts from Petiveria alliacea (Phytolaccaceae) in leukemia and breast cancer treatment in the Caribbean, Central and South America. Herein, we validated the biological activity of a Petiveria alliacea fraction using a metastatic breast adenocarcinoma model (4T1). Petiveria alliacea fraction biological activity was determined estimating cell proliferation, cell colony growth capacity and apoptosis (caspase-3 activity, DNA fragmentation and mitochondrial membrane potential) in 4T1 cells. Petiveria alliacea was used at IC₅₀ concentration (29 µg/mL) and 2 dilutions below, doxorubicin at 0.27 µg/mL (positive control) and dibenzyl disulfide at 2.93 µg/mL (IC50 fraction marker compound). Proteomic estimations were analyzed by LC-MS-MS. Protein level expression was confirmed by RT-PCR. Glucose and lactate levels were measured by enzymatic assays. LD50 was established in BALB/c mice and antitumoral activity evaluated in mice transplanted with GFP-tagged 4T1 cells. Mice were treated with Petiveria alliacea fraction via I.P (182 mg/kg corresponding to 1/8 of LD₅₀ and 2 dilutions below). Petiveria alliacea fraction in vitro induces 4T1 cells apoptosis, caspase-3 activation, DNA fragmentation without mitochondria membrane depolarization, and decreases cell colony growth capacity. Also, changes in glycolytic enzymes expression cause a decrease in glucose uptake and lactate production. Fraction also promotes breast primary tumor regression in BALB/c mice transplanted with GFP-tagged 4T1 cells. A fraction of Petiveria alliacea leaves and stems induces in vitro cell death and in vivo tumor regression in a murine breast cancer model. Our results validate in partly, the traditional use of Petiveria alliacea in breast cancer treatment, revealing a new way of envisioning Petiveria alliacea biological activity. The fraction effect on the glycolytic pathway enzymes contributes to explain the antiproliferative and antitumor activities

  15. Vulnerability of network of networks

    Science.gov (United States)

    Havlin, S.; Kenett, D. Y.; Bashan, A.; Gao, J.; Stanley, H. E.

    2014-10-01

    Our dependence on networks - be they infrastructure, economic, social or others - leaves us prone to crises caused by the vulnerabilities of these networks. There is a great need to develop new methods to protect infrastructure networks and prevent cascade of failures (especially in cases of coupled networks). Terrorist attacks on transportation networks have traumatized modern societies. With a single blast, it has become possible to paralyze airline traffic, electric power supply, ground transportation or Internet communication. How, and at which cost can one restructure the network such that it will become more robust against malicious attacks? The gradual increase in attacks on the networks society depends on - Internet, mobile phone, transportation, air travel, banking, etc. - emphasize the need to develop new strategies to protect and defend these crucial networks of communication and infrastructure networks. One example is the threat of liquid explosives a few years ago, which completely shut down air travel for days, and has created extreme changes in regulations. Such threats and dangers warrant the need for new tools and strategies to defend critical infrastructure. In this paper we review recent advances in the theoretical understanding of the vulnerabilities of interdependent networks with and without spatial embedding, attack strategies and their affect on such networks of networks as well as recently developed strategies to optimize and repair failures caused by such attacks.

  16. Global Metabolic Engineering of Glycolytic Pathway via Multicopy Integration in Saccharomyces cerevisiae.

    Science.gov (United States)

    Yamada, Ryosuke; Wakita, Kazuki; Ogino, Hiroyasu

    2017-04-21

    The use of renewable feedstocks for producing biofuels and biobased chemicals by engineering metabolic pathways of yeast Saccharomyces cerevisiae has recently become an attractive option. Many researchers attempted to increase glucose consumption rate by overexpressing some glycolytic enzymes because most target biobased chemicals are derived through glycolysis. However, these attempts have met with little success. In this study, to create a S. cerevisiae strain with high glucose consumption rate, we used multicopy integration to develop a global metabolic engineering strategy. Among approximately 350 metabolically engineered strains, YPH499/dPdA3-34 exhibited the highest glucose consumption rate. This strain showed 1.3-fold higher cell growth rate and glucose consumption rate than the control strain. Real-time PCR analysis revealed that transcription levels of glycolysis-related genes such as HXK2, PFK1, PFK2, PYK2, PGI1, and PGK1 in YPH499/dPdA3-34 were increased. Our strategy is thus a promising approach to optimize global metabolic pathways in S. cerevisiae.

  17. GLYCOLYTIC AND ENERGETIC RESOURCES AS THE DETERMINANTS OF MEAT QUALITY OF DUROC FATTENERS

    Directory of Open Access Journals (Sweden)

    Andrzej Zybert

    2015-03-01

    Full Text Available The aim of this study was to determine the diagnostic value of glycolityc and energetical quantities on selected meat quality characteristics of Duroc fatteners. A total of 40 Duroc porkers were investigated. Among two analysed sets of determinants (R1 with glycogen and R1 with lactate measured in 45 min after slaughter, the best diagnostic value for meat quality characteristics exhibit a set involving R1 and glycogen that composed determination coefficient (RC2 was 0.66 for pH measured in 45 min up to 144 h post mortem. Also, with currently used meat diagnostic methods the most useful one that explains the glycolytic and energetic quantities in the highest degree is method that exploits 5 determinants, i.e. pH1, pH24, EC2, EC24 and L*24. Higher composed determination coefficient and canonical correlation (CR for this method was obtained for set involving R1 and lactate – 0.41 and 0.64** respectively.

  18. Metabolic Plasiticy in Cancers—Distinct Role of Glycolytic Enzymes GPI, LDHs or Membrane Transporters MCTs

    Directory of Open Access Journals (Sweden)

    Maša Ždralević

    2017-12-01

    Full Text Available Research on cancer metabolism has recently re-surfaced as a major focal point in cancer field with a reprogrammed metabolism no longer being considered as a mere consequence of oncogenic transformation, but as a hallmark of cancer. Reprogramming metabolic pathways and nutrient sensing is an elaborate way by which cancer cells respond to high bioenergetic and anabolic demands during tumorigenesis. Thus, inhibiting specific metabolic pathways at defined steps should provide potent ways of arresting tumor growth. However, both animal models and clinical observations have revealed that this approach is seriously limited by an extraordinary cellular metabolic plasticity. The classical example of cancer metabolic reprogramming is the preference for aerobic glycolysis, or Warburg effect, where cancers increase their glycolytic flux and produce lactate regardless of the presence of the oxygen. This allows cancer cells to meet the metabolic requirements for high rates of proliferation. Here, we discuss the benefits and limitations of disrupting fermentative glycolysis for impeding tumor growth at three levels of the pathway: (i an upstream block at the level of the glucose-6-phosphate isomerase (GPI, (ii a downstream block at the level of lactate dehydrogenases (LDH, isoforms A and B, and (iii the endpoint block preventing lactic acid export (MCT1/4. Using these examples of genetic disruption targeting glycolysis studied in our lab, we will discuss the responses of different cancer cell lines in terms of metabolic rewiring, growth arrest, and tumor escape and compare it with the broader literature.

  19. Merkel Cell Polyomavirus Small T Antigen Promotes Pro-Glycolytic Metabolic Perturbations Required for Transformation.

    Directory of Open Access Journals (Sweden)

    Christian Berrios

    2016-11-01

    Full Text Available Merkel cell polyomavirus (MCPyV is an etiological agent of Merkel cell carcinoma (MCC, a highly aggressive skin cancer. The MCPyV small tumor antigen (ST is required for maintenance of MCC and can transform normal cells. To gain insight into cellular perturbations induced by MCPyV ST, we performed transcriptome analysis of normal human fibroblasts with inducible expression of ST. MCPyV ST dynamically alters the cellular transcriptome with increased levels of glycolytic genes, including the monocarboxylate lactate transporter SLC16A1 (MCT1. Extracellular flux analysis revealed increased lactate export reflecting elevated aerobic glycolysis in ST expressing cells. Inhibition of MCT1 activity suppressed the growth of MCC cell lines and impaired MCPyV-dependent transformation of IMR90 cells. Both NF-κB and MYC have been shown to regulate MCT1 expression. While MYC was required for MCT1 induction, MCPyV-induced MCT1 levels decreased following knockdown of the NF-κB subunit RelA, supporting a synergistic activity between MCPyV and MYC in regulating MCT1 levels. Several MCC lines had high levels of MYCL and MYCN but not MYC. Increased levels of MYCL was more effective than MYC or MYCN in increasing extracellular acidification in MCC cells. Our results demonstrate the effects of MCPyV ST on the cellular transcriptome and reveal that transformation is dependent, at least in part, on elevated aerobic glycolysis.

  20. Restructuring the US telecommunications industry - Impact on innovation

    Science.gov (United States)

    Agnew, C. E.; Romeo, A. A.

    1981-01-01

    The Communications Act of 1934, which regulates the United States telecommunications industry, is becoming less able to deal with the changes brought by modern technology. Therefore, proposals are being made to restructure the industry. Breaking up AT&T into smaller firms would have only minor effects, since its ratio of R and D to sales has been near the median for large telephone companies. Restructuring AT&T into subsidiaries dealing at arms' length might cause a reordering of priorities toward more directly marketable and useful products, but too extensive a break-up could endanger Bell Labs' commitment to basic research. Regulation leads a firm to ignore true social value of possible products, expand into low profit markets, and favor capital-intensive over labor-intensive innovation. If regulatory lag occurs, a firm will produce cost-reducing innovation in order to increase its profit.

  1. Transmission investment and expansion planning in a restructured electricity market

    International Nuclear Information System (INIS)

    Wu, F.F; Wen, F.S.; Zheng, F.L.

    2006-01-01

    Transmission planning in a restructured electricity market becomes increasingly complicated. To bridge the gap between economic and engineering considerations, this survey paper suggests a framework to clarify the interactions among various economic and engineering issues by reviewing recent theoretical and practical progress in transmission investment and transmission planning methodology. Thus, the paper makes economic literature more accessible to the engineering community and engineering literature more accessible to the economic community interested in the subject. (author)

  2. Soft restructuring process in metallurgical enterprises in Poland

    Directory of Open Access Journals (Sweden)

    B. Gajdzik

    2015-10-01

    Full Text Available This article presents the range and outcomes of soft restructuring in metallurgical enterprises in Poland. The term ‘soft restructuring’ applies to changes in metallurgical enterprises’ employment policy during the period of political transformation in Poland. Steelworks performance under the market economy conditions demanded introducing changes in staff resources. Changes referred both to the staff structure as well as employees’ skills and gradual engaging of the staff in building the steelworks’ competitive advantage.

  3. Genomic restructuring in Hordeum chilense durum wheat hybrids ...

    Indian Academy of Sciences (India)

    ANDREIA DELGADO

    [Delgado A., Carvalho A., Martín A. C., Martín A. and Lima-Brito J. 2017 Genomic restructuring in F1 Hordeum chilense × durum wheat hybrids and corresponding hexaploid tritordeum lines revealed by DNA fingerprinting analyses. J. Genet. 96, e13–e24. Online only: http://www.ias.ac.in/jgenet/OnlineResources/96/e13.pdf ].

  4. Electric restructuring: Observations about what is in the public interest

    Energy Technology Data Exchange (ETDEWEB)

    Hoecker, J.

    1996-12-31

    Opinions regarding restructuring of the U.S. electric utility industry are presented in the paper. A brief assessment is made of Federal Energy Regulatory Commission orders requiring open access transmission services and open access same-time information systems. Three subtopics are pursued in some detail: competition between renewables and conservation, the role of government, and the impact of government on the market for renewables. It is concluded that renewable programs can be incorporated into competitive markets through regulatory agencies.

  5. Legal possibilities concerning restructuring of companies in business difficulties

    OpenAIRE

    Čuveljak, Jelena; Rašić, Mario

    2017-01-01

    Since difficulties during business are an integral part of entrepreneurship, the Croatian legislative offers several restructuring models for companies who have business issues. The pre-bankruptcy agreement is available in cases when the debtor is threatened by insolvency and during this procedure, the business of the company is still managed by the existing management board. However, it is necessary that the pre-bankruptcy procedure is finished within a short deadline. The bankruptcy procedu...

  6. The impact of electricity restructuring on the natural gas industry

    International Nuclear Information System (INIS)

    Given, G.

    1999-03-01

    The main objective of the study is to quantify the impact of electrical restructuring on the natural gas industry, in particular the expected rise in natural gas consumption for electricity generation in Canada from 2000 to 2020. To this end, the study estimates how large the demand potential is and where it is likely to materialize, with the timing of these events also considered. Chapter two discusses the study methodology, which is that of a quantitative forecast. Before an analytical model was chosen, a careful review of potential candidates, a literature review, and an analysis of important issues were undertaken. Chapter three provides requisite background information of electricity restructuring issues. U.K. experiences are highlighted, and common issues and differences among states and provinces are discussed also. Chapter four provides some necessary historical background and puts into perspective the importance of gas consumption for electric generation. Also treated are relevant Canadian and U.S. generation and capacity, and forecasts of restructuring impacts from other organizations. Whether a consensus forecast for gas demand exists is examined, as well as a number of critical factors examined in various studies. Chapter five describes the development of individual study scenarios and key assumptions contained in each scenario. Also described are some of the selected model's capabilities. Retail and generation company strategy parameters are highlighted to provide a better understanding of those capabilities. The competition parameters chosen for generators and retail companies in the model are described. Chapter six reports all key forecast variables in a discussion which compares relevant scenario and sensitivity forecast results. Detailed forecast results for two scenarios are provided in appendices. Chapter seven discusses new investment and business opportunities and challenges in light of the forecast results. These are reviewed for a number of

  7. Cognitive restructuring of gambling-related thoughts: A systematic review.

    Science.gov (United States)

    Chrétien, Maxime; Giroux, Isabelle; Goulet, Annie; Jacques, Christian; Bouchard, Stéphane

    2017-12-01

    Gamblers' thoughts have a fundamental influence on their gambling problem. Cognitive restructuring is the intervention of choice to correct those thoughts. However, certain difficulties are noted in the application of cognitive restructuring techniques and the comprehension of their guidelines. Furthermore, the increase of skill game players (e.g. poker) entering treatment creates a challenge for therapists, as these gamblers present with different thoughts than those of the gamblers usually encountered in treatment (e.g. chance-only games like electronic gambling machines). This systematic review aims to describe how cognitive restructuring is carried out with gamblers based on the evidence available in empirical studies that include cognitive interventions for gambling. Of the 2607 studies collected, 39 were retained. The results highlight exposure as the most frequently used technique to facilitate identification of gambling-related thoughts (imaginal=28.2%; in vivo=10.3%). More than half of the studies (69.2%) clearly reported therapeutic techniques aimed to correct gamblers' thoughts, of which 37% involved visual support to challenge those thoughts (e.g. ABC log). Of the 39 studies retained, 48.7% included skill game players (i.e., poker, blackjack, sports betting) in their sample. However, none of these studies mentioned whether cognitive restructuring had been adapted for these gamblers. Several terms referring to gamblers' thoughts were used interchangeably (e.g. erroneous, dysfunctional or inadequate thoughts), although each of these terms could refer to specific content. Clinical implications of the results are discussed with regard to the needs of therapists. This review also suggests recommendations for future research. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Overexpression of Genes Encoding Glycolytic Enzymes in Corynebacterium glutamicum Enhances Glucose Metabolism and Alanine Production under Oxygen Deprivation Conditions

    Science.gov (United States)

    Yamamoto, Shogo; Gunji, Wataru; Suzuki, Hiroaki; Toda, Hiroshi; Suda, Masako; Jojima, Toru; Inui, Masayuki

    2012-01-01

    We previously reported that Corynebacterium glutamicum strain ΔldhAΔppc+alaD+gapA, overexpressing glyceraldehyde-3-phosphate dehydrogenase-encoding gapA, shows significantly improved glucose consumption and alanine formation under oxygen deprivation conditions (T. Jojima, M. Fujii, E. Mori, M. Inui, and H. Yukawa, Appl. Microbiol. Biotechnol. 87:159–165, 2010). In this study, we employ stepwise overexpression and chromosomal integration of a total of four genes encoding glycolytic enzymes (herein referred to as glycolytic genes) to demonstrate further successive improvements in C. glutamicum glucose metabolism under oxygen deprivation. In addition to gapA, overexpressing pyruvate kinase-encoding pyk and phosphofructokinase-encoding pfk enabled strain GLY2/pCRD500 to realize respective 13% and 20% improved rates of glucose consumption and alanine formation compared to GLY1/pCRD500. Subsequent overexpression of glucose-6-phosphate isomerase-encoding gpi in strain GLY3/pCRD500 further improved its glucose metabolism. Notably, both alanine productivity and yield increased after each overexpression step. After 48 h of incubation, GLY3/pCRD500 produced 2,430 mM alanine at a yield of 91.8%. This was 6.4-fold higher productivity than that of the wild-type strain. Intracellular metabolite analysis showed that gapA overexpression led to a decreased concentration of metabolites upstream of glyceraldehyde-3-phosphate dehydrogenase, suggesting that the overexpression resolved a bottleneck in glycolysis. Changing ratios of the extracellular metabolites by overexpression of glycolytic genes resulted in reduction of the intracellular NADH/NAD+ ratio, which also plays an important role on the improvement of glucose consumption. Enhanced alanine dehydrogenase activity using a high-copy-number plasmid further accelerated the overall alanine productivity. Increase in glycolytic enzyme activities is a promising approach to make drastic progress in growth-arrested bioprocesses. PMID

  9. American Indian tribes and electric industry restructuring: Issues and opportunities

    Energy Technology Data Exchange (ETDEWEB)

    Howarth, D. [Morse, Richard, and Weisenmiller, and Associates Inc., Oakland, CA (United States); Busch, J. [Lawrence Berkeley National Lab., CA (United States); Starrs, T. [Kelso, Starrs, and Associates LLC, Vashon, WA (United States)

    1997-07-01

    The US electric utility industry is undergoing a period of fundamental change that has significant implications for Native American tribes. Although many details remain to be determined, the future electric power industry will be very different from that of the present. It is anticipated that the new competitive electric industry will be more efficient, which some believe will benefit all participants by lowering electricity costs. Recent developments in the industry, however, indicate that the restructuring process will likely benefit some parties at the expense of others. Given the historical experience and current situation of Native American tribes in the US, there is good reason to pay attention to electric industry changes to ensure that the situation of tribes is improved and not worsened as a result of electric restructuring. This paper provides a review of electricity restructuring in the US and identifies ways in which tribes may be affected and how tribes may seek to protect and serve their interests. Chapter 2 describes the current status of energy production and service on reservations. Chapter 3 provides an overview of the evolution of the electric industry to its present form and introduces the regulatory and structural changes presently taking place. Chapter 4 provides a more detailed discussion of changes in the US electric industry with a specific focus on the implications of these changes for tribes. Chapter 5 presents a summary of the conclusions reached in this paper.

  10. Power market restructuring in Asia : Russia, China, India, and Japan

    International Nuclear Information System (INIS)

    Hammons, T.J.; Zhong, J.; Mukhopadhyay, S.; Kurihara, I.

    2008-01-01

    Many countries are now in the process of deregulating their power industries in order to promote growth and competitiveness. This paper discussed power market restructuring activities in Russia, China, India and Japan. Economic convergence points in Russian and Asian power markets were reviewed. The state of Russia and China's power industry after the implementation of recent power restructuring initiatives was discussed. Technical characteristics of the industries were evaluated, and market development plans were outlined. Regional electricity markets in Asia were discussed, as well as issues related to domestic and foreign investment. Institutional reforms were reviewed, and individual outlines of revisions for the power industries of the 4 countries were presented. The study demonstrated that structuring processes vary from country to country. Differences in restructuring patterns were attributed to economic differences; country-specific features established within the electric power industry; and attitudes towards deregulation. It was concluded that the reforms adopted by the countries will lead to the expansion of national electric power systems. 23 refs., 4 tabs., 6 figs

  11. Restructuring and energy efficiency improvement of the Bulgarian energy economy

    International Nuclear Information System (INIS)

    Moumdjian, G.

    1993-01-01

    The structure of the national energy economy of Bulgaria implies characteristic features that specify low efficiency as regards power production, ecology and economics. Even the qualitative assessments show that these indices stand far away from the standards established in developed countries like Denmark, Finland, Sweden, etc. The best starting position for harmful energy efficiency improvement as well as emission reduction must be based on the restructuring of energy economy. The strategy of restructuring and development of energy economy covers the whole integrated national energy flow system 'resources - end user'. The preliminary study shows that energy efficiency can be increased by 25-30% within a period of 6-10 years using the least-cost investment strategy (including the research and development activities expenses). The study covers the existing structure of energy sector. Scenarios are being elaborated for its development and restructuring in respect to: heat production and transfer; electricity generation and transmission; energy consumption and conservation in residential buildings, public buildings and commercial sector; energy consumption in transport sector and agriculture. The approach for identification of the real potential opportunities in relation to the above stated areas is based on mathematical statistics and stochastic differential equations, multicriterial assessments, approach of self organisation systems and demand-side management. (author)

  12. On program restructuring, scheduling, and communication for parallel processor systems

    Energy Technology Data Exchange (ETDEWEB)

    Polychronopoulos, Constantine D. [Univ. of Illinois, Urbana, IL (United States)

    1986-08-01

    This dissertation discusses several software and hardware aspects of program execution on large-scale, high-performance parallel processor systems. The issues covered are program restructuring, partitioning, scheduling and interprocessor communication, synchronization, and hardware design issues of specialized units. All this work was performed focusing on a single goal: to maximize program speedup, or equivalently, to minimize parallel execution time. Parafrase, a Fortran restructuring compiler was used to transform programs in a parallel form and conduct experiments. Two new program restructuring techniques are presented, loop coalescing and subscript blocking. Compile-time and run-time scheduling schemes are covered extensively. Depending on the program construct, these algorithms generate optimal or near-optimal schedules. For the case of arbitrarily nested hybrid loops, two optimal scheduling algorithms for dynamic and static scheduling are presented. Simulation results are given for a new dynamic scheduling algorithm. The performance of this algorithm is compared to that of self-scheduling. Techniques for program partitioning and minimization of interprocessor communication for idealized program models and for real Fortran programs are also discussed. The close relationship between scheduling, interprocessor communication, and synchronization becomes apparent at several points in this work. Finally, the impact of various types of overhead on program speedup and experimental results are presented.

  13. A restructuring of CF package for MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S. H.; Kim, K. R.; Kim, D. H.; Cho, S. W.

    2004-01-01

    CF package, which evaluates user-specified 'control functions' and applies them to define or control various aspects of computation, has been restructured for the MIDAS computer code. MIDAS is being developed as an integrated severe accident analysis code with a user-friendly graphical user interface and modernized data structure. To do this, data transferring methods of current MELCOR code are modified and adopted into the CF package. The data structure of the current MELCOR code using FORTRAN77 causes a difficult grasping of meaning of the variables as well as waste of memory, difficulty is more over because its data is location information of other package's data due to characteristics of CF package. New features of FORTRAN90 make it possible to allocate the storage dynamically and to use the user-defined data type, which lead to an efficient memory treatment and an easy understanding of the code. Restructuring of the CF package addressed in this paper includes module development, subroutine modification, and treats MELGEN, which generates data file, as well as MELCOR, which is processing a calculation. The verification has been done by comparing the results of the modified code with those from the existing code. As the trends are similar to each other, it hints that the same approach could be extended to the entire code package. It is expected that code restructuring will accelerate the code domestication thanks to direct understanding of each variable and easy implementation of modified or newly developed models

  14. Glycolytic fast-twitch muscle fiber restoration counters adverse age-related changes in body composition and metabolism.

    Science.gov (United States)

    Akasaki, Yuichi; Ouchi, Noriyuki; Izumiya, Yasuhiro; Bernardo, Barbara L; Lebrasseur, Nathan K; Walsh, Kenneth

    2014-02-01

    Aging is associated with the development of insulin resistance, increased adiposity, and accumulation of ectopic lipid deposits in tissues and organs. Starting in mid-life there is a progressive decline in lean muscle mass associated with the preferential loss of glycolytic, fast-twitch myofibers. However, it is not known to what extent muscle loss and metabolic dysfunction are causally related or whether they are independent epiphenomena of the aging process. Here, we utilized a skeletal-muscle-specific, conditional transgenic mouse expressing a constitutively active form of Akt1 to examine the consequences of glycolytic, fast-twitch muscle growth in young vs. middle-aged animals fed standard low-fat chow diets. Activation of the Akt1 transgene led to selective skeletal muscle hypertrophy, reversing the loss of lean muscle mass observed upon aging. The Akt1-mediated increase in muscle mass led to reductions in fat mass and hepatic steatosis in older animals, and corrected age-associated impairments in glucose metabolism. These results indicate that the loss of lean muscle mass is a significant contributor to the development of age-related metabolic dysfunction and that interventions that preserve or restore fast/glycolytic muscle may delay the onset of metabolic disease. © 2013 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

  15. Glycolytic and Mitochondrial Metabolism in Pancreatic Islets from MSG-Treated Obese Rats Subjected to Swimming Training

    Directory of Open Access Journals (Sweden)

    Nayara de Carvalho Leite

    2013-02-01

    Full Text Available Backgrounds/Aims: Obese rats obtained by neonatal monosodium glutamate (MSG administration present insulin hypersecretion. The metabolic mechanism by which glucose catabolism is coupled to insulin secretion in the pancreatic β-cells from MSG-treated rats is understood. The purpose of this study was to evaluate glucose metabolism in pancreatic islets from MSG-treated rats subjected to swimming training. Methods: MSG-treated and control (CON rats swam for 30 minutes (3 times/week over a period of 10 weeks. Pancreatic islets were isolated and incubated with glucose in the presence of glycolytic or mitochondrial inhibitors. Results: Swimming training attenuated fat pad accumulation, avoiding changes in the plasma levels of lipids, glucose and insulin in MSG-treated rats. Adipocyte and islet hypertrophy observed in MSG-treated rats were attenuated by exercise. Pancreatic islets from MSG-treated obese rats also showed insulin hypersecretion, greater glucose transporter 2 (GLUT2 expression, increased glycolytic flux and reduced mitochondrial complex III activity. Conclusion: Swimming training attenuated islet hypertrophy and normalised GLUT2 expression, contributing to a reduction in the glucose responsiveness of pancreatic islets from MSG-treated rats without altering glycolytic flux. However, physical training increased the activity of mitochondrial complex III in pancreatic islets from MSG-treated rats without a subsequent increase in glucose-induced insulin secretion.

  16. Lithium prevents early cytosolic calcium increase and secondary injurious calcium overload in glycolytically inhibited endothelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Bosche, Bert, E-mail: bert.bosche@uk-essen.de [Department of Neurology, University of Duisburg-Essen (Germany); Max Planck Institute for Neurological Research with Klaus-Joachim-Zülch Laboratories of the Max Planck Society and the Medical Faculty of the University of Cologne (Germany); Schäfer, Matthias, E-mail: matthias.schaefer@sanofi.com [Institute of Physiology, Justus-Liebig-University Giessen (Germany); Graf, Rudolf, E-mail: rudolf.graf@nf.mpg.de [Max Planck Institute for Neurological Research with Klaus-Joachim-Zülch Laboratories of the Max Planck Society and the Medical Faculty of the University of Cologne (Germany); Härtel, Frauke V., E-mail: frauke.haertel@tu-dresden.de [Institute of Physiology, Medical Faculty Carl Gustav Carus, Technical University Dresden (Germany); Schäfer, Ute, E-mail: ute.schaefer@medunigraz.at [Research Unit for Experimental Neurotraumatology, Medical University of Graz (Austria); Noll, Thomas, E-mail: thomas.noll@tu-dresden.de [Institute of Physiology, Medical Faculty Carl Gustav Carus, Technical University Dresden (Germany)

    2013-05-03

    Highlights: •We investigate free calcium as a central signalling element in endothelial cells. •Inhibition of glycolysis with 2-deoxy-D-glucose reduces cellular ATP. •This manoeuvre leads to a biphasic increase and overload of free calcium. •Pre-treatment with lithium for 24 h abolishes both phases of the calcium increase. •This provides a new strategy to protect endothelial calcium homeostasis and barrier function. -- Abstract: Cytosolic free calcium concentration ([Ca{sup 2+}]{sub i}) is a central signalling element for the maintenance of endothelial barrier function. Under physiological conditions, it is controlled within narrow limits. Metabolic inhibition during ischemia/reperfusion, however, induces [Ca{sup 2+}]{sub i} overload, which results in barrier failure. In a model of cultured porcine aortic endothelial monolayers (EC), we addressed the question of whether [Ca{sup 2+}]{sub i} overload can be prevented by lithium treatment. [Ca{sup 2+}]{sub i} and ATP were analysed using Fura-2 and HPLC, respectively. The combined inhibition of glycolytic and mitochondrial ATP synthesis by 2-desoxy-D-glucose (5 mM; 2-DG) plus sodium cyanide (5 mM; NaCN) caused a significant decrease in cellular ATP content (14 ± 1 nmol/mg protein vs. 18 ± 1 nmol/mg protein in the control, n = 6 culture dishes, P < 0.05), an increase in [Ca{sup 2+}]{sub i} (278 ± 24 nM vs. 71 ± 2 nM in the control, n = 60 cells, P < 0.05), and the formation of gaps between adjacent EC. These observations indicate that there is impaired barrier function at an early state of metabolic inhibition. Glycolytic inhibition alone by 10 mM 2-DG led to a similar decrease in ATP content (14 ± 2 nmol/mg vs. 18 ± 1 nmol/mg in the control, P < 0.05) with a delay of 5 min. The [Ca{sup 2+}]{sub i} response of EC was biphasic with a peak after 1 min (183 ± 6 nM vs. 71 ± 1 nM, n = 60 cells, P < 0.05) followed by a sustained increase in [Ca{sup 2+}]{sub i}. A 24-h pre-treatment with 10 mM of lithium

  17. The Glycolytic Versatility of Bacteroides uniformis CECT 7771 and Its Genome Response to Oligo and Polysaccharides

    Directory of Open Access Journals (Sweden)

    Alfonso Benítez-Páez

    2017-08-01

    Full Text Available Bacteroides spp. are dominant components of the phylum Bacteroidetes in the gut microbiota and prosper in glycan enriched environments. However, knowledge of the machinery of specific species isolated from humans (like Bacteroides uniformis contributing to the utilization of dietary and endogenous sources of glycans and their byproducts is limited. We have used the cutting-edge nanopore-based technology to sequence the genome of B. uniformis CECT 7771, a human symbiont with a proven pre-clinical efficacy on metabolic and immune dysfunctions in obesity animal models. We have also used massive sequencing approaches to distinguish the genome expression patterns in response to carbon sources of different complexity during growth. At genome-wide level, our analyses globally demonstrate that B. uniformis strains exhibit an expanded glycolytic capability when compared with other Bacteroides species. Moreover, by studying the growth and whole-genome expression of B. uniformis CECT 7771 in response to different carbon sources, we detected a differential growth fitness and expression patterns across the genome depending on the carbon source of the culture media. The dietary fibers used exerted different effects on B. uniformis CECT 7771 activating different molecular pathways and, therefore, allowing the production of different metabolite types with potential impact on gut health. The genome and transcriptome analysis of B. uniformis CECT 7771, in response to different carbon sources, shows its high versatility to utilize both dietary and endogenous glycans along with the production of potentially beneficial end products for both the bacterium and the host, pointing to a mechanistic basis of a mutualistic relationship.

  18. Glycolytic and chitinolytic activities of Phlebotomus papatasi (Diptera: Psychodidae) from diverse ecological habitats.

    Science.gov (United States)

    Jacobson, Raymond L; Studentsky, Liora; Schlein, Yosef

    2007-11-01

    The sand fly Phlebotomus papatasi Scopoli, 1786, the vector of Leishmania major Yakimoff et Schokhor, 1914, is found in desert areas where sugars are scarce but also in habitats that abound in sugar sources. The sand flies require sugar meals from plant sources for their energy requirements and to hydrolyze these complex sugars, they need a repertoire of glycosidases. We presumed that there are differences in the levels of glycosidase activities in flies from such habitats and also assumed that they may be instrumental in modulating the flies' susceptibility to L. major infections. Phlebotomus papatasi originating from diverse ecological habitats ranging from an oasis to desert sites were colonized. They were analyzed for weight changes and glycosidase activities before and after feeding on 1M sucrose solution. Oasis flies were smaller than desert flies but took larger sugar meals. Homogenates of these flies hydrolyzed 16 synthetic and 2 natural glycoside substrates to varying degrees. The arid-region flies tended to produce more glycosidase activity than those originating in sugar-rich environments, especially sucrase, alpha- and beta-glucosidase, aalpha-fucosidase, alpha-mannosidase, and alpha- and beta-N-acetylgalactosaminidase. However, chitinolytic enzyme activities and particularly the beta-N-acetylhexosaminidase activity of oasis flies were higher than other flies tested. In comparing the desert flies, there were also significant differences in glycolytic enzyme activities between the spring-line (flowering season) of flies and the autumn-line (end of dry season) flies. A range of saccharide inhibitors was tested to demonstrate the specificity of the enzymes.

  19. Arsenite stimulates glutathione export and glycolytic flux in viable primary rat brain astrocytes.

    Science.gov (United States)

    Tadepalle, Nimesha; Koehler, Yvonne; Brandmann, Maria; Meyer, Nils; Dringen, Ralf

    2014-10-01

    Intoxication with inorganic arsenicals leads to neuropathies and impaired cognitive functions. However, little is known so far on the cellular targets that are involved in the adverse effects of arsenite to brain cells. To test whether arsenite may affect neural glucose and glutathione (GSH) metabolism, primary astrocyte cultures from rat brain were used as a model system. Exposure of cultured astrocytes to arsenite in concentrations of up to 0.3mM did not compromise cell viability during incubations for up to 6h, while 1mM arsenite damaged the cells already within 2h after application. Determination of cellular arsenic contents of astrocytes that had been incubated for 2h with arsenite revealed an almost linear concentration-dependent increase in the specific cellular arsenic content. Exposure of astrocytes to arsenite stimulated the export of GSH and accelerated the cellular glucose consumption and lactate production in a time- and concentration-dependent manner. Half-maximal stimulation of GSH export and glycolytic flux were observed for arsenite in concentrations of 0.1mM and 0.3mM, respectively. The arsenite-induced stimulation of both processes was abolished upon removal of extracellular arsenite. The strong stimulation of GSH export by arsenite was prevented by MK571, an inhibitor of the multidrug resistance protein 1, suggesting that this transporter mediates the accelerated GSH export. In addition, presence of MK571 significantly increased the specific cellular arsenic content, suggesting that Mrp1 may also be involved in arsenic export from astrocytes. The data observed suggest that alterations in glucose and GSH metabolism may contribute to the reported adverse neural consequences of intoxication with arsenite. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Networking

    OpenAIRE

    Rauno Lindholm, Daniel; Boisen Devantier, Lykke; Nyborg, Karoline Lykke; Høgsbro, Andreas; Fries, de; Skovlund, Louise

    2016-01-01

    The purpose of this project was to examine what influencing factor that has had an impact on the presumed increasement of the use of networking among academics on the labour market and how it is expressed. On the basis of the influence from globalization on the labour market it can be concluded that the globalization has transformed the labour market into a market based on the organization of networks. In this new organization there is a greater emphasis on employees having social qualificati...

  1. Low-income energy policy in a restructuring electricity industry: an assessment of federal options

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, L.W.

    1997-07-01

    This report identifies both the low-income energy services historically provided in the electricity industry and those services that may be affected by industry restructuring. It identifies policies that are being proposed or could be developed to address low- income electricity services in a restructured industry. It discusses potential federal policy options and identifies key policy and implementation issues that arise when considering these potential federal initiatives. To understand recent policy development at the state level, we reviewed restructuring proposals from eight states and the accompanying testimony and comments filed in restructuring proceedings in these states.

  2. International company restructuring and the effects on high-skilled employees in lead companies

    DEFF Research Database (Denmark)

    Hansen, Nana Wesley

    2016-01-01

    International company restructuring involving knowledge intensive jobs causes increasing concern about the effect on the globalization of high-skilled employees. Drawing on case studies in four Danish-based companies, this article argues that high-skilled employees in lead companies are rarely...... insulated from international restructuring processes. However, effects on wage and working conditions vary dependent on the motivation for restructuring, and as companies learn to take advantage of pools of skilled employees abroad. Continued international restructuring appear to challenge the insulation...

  3. Strategic plan for the restructured US fusion energy sciences program

    International Nuclear Information System (INIS)

    1996-08-01

    This plan reflects a transition to a restructured fusion program, with a change in focus from an energy technology development program to a fusion energy sciences program. Since the energy crisis of the early 1970's, the U.S. fusion program has presented itself as a goal- oriented fusion energy development program, with milestones that required rapidly increasing budgets. The Energy Policy Act of 1992 also called for a goal-oriented development program consistent with the Department's planning. Actual funding levels, however, have forced a premature narrowing of the program to the tokamak approach. By 1995, with no clear, immediate need driving the schedule for developing fusion energy and with enormous pressure to reduce discretionary spending, Congress cut fusion program funding for FY 1996 by one-third and called for a major restructuring of the program. Based on the recommendations of the Fusion Energy Advisory Committee (FEAC), the Department has decided to pursue a program that concentrates on world-class plasma, science, and on maintaining an involvement in fusion energy science through international collaboration. At the same time, the Japanese and Europeans, with energy situations different from ours, are continuing with their goal- oriented fusion programs. Collaboration with them provides a highly leveraged means of continued involvement in fusion energy science and technology, especially through participation in the engineering and design activities of the International Thermonuclear Experimental Reactor program, ITER. This restructured fusion energy sciences program, with its focus on fundamental fusion science and technology, may well provide insights that lead to more attractive fusion power plants, and will make use of the scientific infrastructure that will allow the United States to launch a fusion energy development program at some future date

  4. SOVEREIGN DEBT RESTRUCTURING AND “VULTURE FUNDS”

    Directory of Open Access Journals (Sweden)

    Emilia Cornelia STOICA

    2016-06-01

    Full Text Available Defining sovereign debt - debt issued or guaranteed by a public entity: central and / or regional public authorities, central banks, public institutions or enterprises - must include the risks that its management may generate, mainly the risk of default. If an medium period of time - 3-5 years – the macroeconomic growth of a state, and as the result the increase of the public revenues constantly lies below the growth of sovereign debt, these will cause an insolvability risk to cover it, and that state should proceed to restructure its debt. Financial stability of public authorities and sovereign debt occurred since the beginning of the creation of democratic states, and instruments for debt restructuring have been continuously adapted to economic and social conjuncture. Initially, states faced a necessity of funding were borrowed from foreign governments and / or large consortia bank, and when their debts had to be restructured it has been created the international institutional framework to negotiate between debtor countries and public creditors - Paris Club - and to coordinate negotiations between public authorities and major debtor consortia - London Club. In the last decade 'vulture funds' occurred, which are hedge funds acquiring from the secondary financial market debt the securities, including public debt, to a much lower share nominal value. Subsequently, vulture funds claim states issuing debt repayment at values close or equal to the face value - in this way can make a profit of more than 100% of the financial investment they made it on the secondary market. If these countries do not comply, generally being unable to honor their public debt, vultures funds act the countries in international courts, which usually prevails because vultures funds’ action is legal under current conditions.

  5. Do generation firms in restructured electricity markets have incentives to support social-welfare-improving transmission investments?

    International Nuclear Information System (INIS)

    Sauma, Enzo E.; Oren, Shmuel S.

    2009-01-01

    This paper examines the incentives that generation firms have in restructured electricity markets for supporting long-term transmission investments. In particular, we study whether generation firms, which arguably play a dominant role in the restructured electricity markets, have the incentives to fund or support incremental social-welfare-improving transmission investments. We examine this question in a two-node network and explore how such incentives are affected by the ownership of financial transmission rights (FTRs) by generation firms. In the analyzed two-node network, we show both (1) that the net exporter generation firm has the correct incentives to increase the transmission capacity incrementally up to a certain level and (2) that, although a policy that allocates FTRs to the net exporter generation firm can be desirable from a social point of view, such a policy would dilute the net-importer-generation-firm's incentives to support transmission expansion. Moreover, if all FTRs were allocated or auctioned off to the net exporter generation firm, then it is possible to increase both consumer surplus and social welfare while keeping the net exporter generation firm revenue neutral. (author)

  6. Hyperoxia decreases glycolytic capacity, glycolytic reserve and oxidative phosphorylation in MLE-12 cells and inhibits complex I and II function, but not complex IV in isolated mouse lung mitochondria.

    Directory of Open Access Journals (Sweden)

    Kumuda C Das

    Full Text Available High levels of oxygen (hyperoxia are frequently used in critical care units and in conditions of respiratory insufficiencies in adults, as well as in infants. However, hyperoxia has been implicated in a number of pulmonary disorders including bronchopulmonary dysplasia (BPD and adult respiratory distress syndrome (ARDS. Hyperoxia increases the generation of reactive oxygen species (ROS in the mitochondria that could impair the function of the mitochondrial electron transport chain. We analyzed lung mitochondrial function in hyperoxia using the XF24 analyzer (extracellular flux and optimized the assay for lung epithelial cells and mitochondria isolated from lungs of mice. Our data show that hyperoxia decreases basal oxygen consumption rate (OCR, spare respiratory capacity, maximal respiration and ATP turnover in MLE-12 cells. There was significant decrease in glycolytic capacity and glycolytic reserve in MLE-12 cells exposed to hyperoxia. Using mitochondria isolated from lungs of mice exposed to hyperoxia or normoxia we have shown that hyperoxia decreased the basal, state 3 and state3 μ (respiration in an uncoupled state respirations. Further, using substrate or inhibitor of a specific complex we show that the OCR via complex I and II, but not complex IV was decreased, demonstrating that complexes I and II are specific targets of hyperoxia. Further, the activities of complex I (NADH dehydrogenase, NADH-DH and complex II (succinate dehydrogenase, SDH were decreased in hyperoxia, but the activity of complex IV (cytochrome oxidase, COX remains unchanged. Taken together, our study show that hyperoxia impairs glycolytic and mitochondrial energy metabolism in in tact cells, as well as in lungs of mice by selectively inactivating components of electron transport system.

  7. Optimized multi area AGC simulation in restructured power systems

    International Nuclear Information System (INIS)

    Bhatt, Praghnesh; Roy, Ranjit; Ghoshal, S.P.

    2010-01-01

    In this paper, the traditional automatic generation control loop with modifications is incorporated for simulating automatic generation control (AGC) in restructured power system. Federal energy regulatory commission (FERC) encourages an open market system for price based operation. FERC has issued a notice for proposed rulemaking of various ancillary services. One of these ancillary services is load following with frequency control which comes broadly under Automatic Generation Control in deregulated regime. The concept of DISCO participation matrix is used to simulate the bilateral contracts in the three areas and four area diagrams. Hybrid particle swarm optimization is used to obtain optimal gain parameters for optimal transient performance. (author)

  8. Growth Trade-Offs Accompany the Emergence of Glycolytic Metabolism in Shewanella oneidensis MR-1.

    Science.gov (United States)

    Chubiz, Lon M; Marx, Christopher J

    2017-06-01

    Bacteria increase their metabolic capacity via the acquisition of genetic material or by the mutation of genes already present in the genome. Here, we explore the mechanisms and trade-offs involved when Shewanella oneidensis , a bacterium that typically consumes small organic and amino acids, rapidly evolves to expand its metabolic capacity to catabolize glucose after a short period of adaptation to a glucose-rich environment. Using whole-genome sequencing and genetic approaches, we discovered that deletions in a region including the transcriptional repressor ( nagR ) that regulates the expression of genes associated with catabolism of N -acetylglucosamine are the common basis for evolved glucose metabolism across populations. The loss of nagR results in the constitutive expression of genes for an N -acetylglucosamine permease ( nagP ) and kinase ( nagK ). We demonstrate that promiscuous activities of both NagP and NagK toward glucose allow for the transport and phosphorylation of glucose to glucose-6-phosphate, the initial events of glycolysis otherwise thought to be absent in S. oneidensis 13 C-based metabolic flux analysis uncovered that subsequent utilization was mediated by the Entner-Doudoroff pathway. This is an example whereby gene loss and preexisting enzymatic promiscuity, and not gain-of-function mutations, were the drivers of increased metabolic capacity. However, we observed a significant decrease in the growth rate on lactate after adaptation to glucose catabolism, suggesting that trade-offs may explain why glycolytic function may not be readily observed in S. oneidensis in natural environments despite it being readily accessible through just a single mutational event. IMPORTANCE Gains in metabolic capacity are frequently associated with the acquisition of novel genetic material via natural or engineered horizontal gene transfer events. Here, we explored how a bacterium that typically consumes small organic acids and amino acids expands its metabolic

  9. WE-FG-BRA-08: Potential Role of the Glycolytic Oscillator in Acute Hypoxia in Tumors

    International Nuclear Information System (INIS)

    Che Fru, L; Adamson, E; Campos, D; Song, C; Kimple, R; Fain, S; Kissick, M; Jacques, S; Kogel, A van der; Nickel, K

    2016-01-01

    Purpose: Oscillatory dynamics in acute hypoxia have been observed, but poorly understood. They have mostly been attributed to vascular perturbations, but no link has yet been made to metabolic causes. We set out to determine the fundamental frequencies and test for coherence in tumor oxygen dynamics and spatial properties. Methods: Severe combined immunodeficient (SCID) mice were inoculated onto bilateral flanks with human derived head and neck carcinoma (UW-SCC22) cell line xenografts. Oxygen dynamics were monitored in the tumor every minute for an hour using three modalities: blood oxygen level dependent - magnetic resonance imaging (BOLD-MRI), hemoglobin oxygen saturation photoacoustic, and locally manufactured optical probes for spectral fitting. A statistical test was used to separate fluctuating from non-fluctuating voxels and pixels in BOLD-MRI and photoacoustic data respectively. The power spectrum density (PSD) and the autocorrelation functions were calculated for the time series of each voxel, pixel and region, of the BOLD-MRI, photoacoustic or fiber optic data respectively. Results: Using all three techniques, intermittent oxygen dynamics with both coherent and incoherent signatures was observed in the tumors. Upon averaging the PSDs of fluctuating voxels and pixels, it was found that these oscillations occurred with periods of minutes to tens of minutes from all three approaches. Observations from the BOLD-MRI and photoacoustic data showed that clusters of voxels oscillated in a synchronized manner. Conclusion: We were able to use three different modalities to show that fluctuation in tumor oxygen is both coherent and incoherent, with periods of minutes to tens of minutes. These periods are very similar to those from the well-established metabolic, non-linear biomechanical phenomenon called the glycolytic oscillator. This may provide an additional explanation to the cause of cyclic hypoxia. Such dynamics could have profound implications in

  10. Effect of hypoxia on the activity and binding of glycolytic and associated enzymes in sea scorpion tissues

    Directory of Open Access Journals (Sweden)

    Lushchak V.I.

    1998-01-01

    Full Text Available The effect of hypoxia on the levels of glycogen, glucose and lactate as well as the activities and binding of glycolytic and associated enzymes to subcellular structures was studied in brain, liver and white muscle of the teleost fish, Scorpaena porcus. Hypoxia exposure decreased glucose levels in liver from 2.53 to 1.70 µmol/g wet weight and in muscle led to its increase from 3.64 to 25.1 µmol/g wet weight. Maximal activities of several enzymes in brain were increased by hypoxia: hexokinase by 23%, phosphoglucoisomerase by 47% and phosphofructokinase (PFK by 56%. However, activities of other enzymes in brain as well as enzymes in liver and white muscle were largely unchanged or decreased during experimental hypoxia. Glycolytic enzymes in all three tissues were partitioned between soluble and particulate-bound forms. In several cases, the percentage of bound enzymes was reduced during hypoxia; bound aldolase in brain was reduced from 36.4 to 30.3% whereas glucose-6-phosphate dehydrogenase fell from 55.7 to 28.7% bound. In muscle PFK was reduced from 57.4 to 41.7% bound. Oppositely, the proportion of bound aldolase and triosephosphate isomerase increased in hypoxic muscle. Phosphoglucomutase did not appear to occur in a bound form in liver and bound phosphoglucomutase disappeared in muscle during hypoxia exposure. Anoxia exposure also led to the disappearance of bound fructose-1,6-bisphosphatase in liver, whereas a bound fraction of this enzyme appeared in white muscle of anoxic animals. The possible function of reversible binding of glycolytic enzymes to subcellular structures as a regulatory mechanism of carbohydrate metabolism is discussed.

  11. Genetic disruption of lactate/H+ symporters (MCTs) and their subunit CD147/BASIGIN sensitizes glycolytic tumor cells to phenformin.

    Science.gov (United States)

    Marchiq, Ibtissam; Le Floch, Renaud; Roux, Danièle; Simon, Marie-Pierre; Pouyssegur, Jacques

    2015-01-01

    Rapidly growing glycolytic tumors require energy and intracellular pH (pHi) homeostasis through the activity of two major monocarboxylate transporters, MCT1 and the hypoxia-inducible MCT4, in intimate association with the glycoprotein CD147/BASIGIN (BSG). To further explore and validate the blockade of lactic acid export as an anticancer strategy, we disrupted, via zinc finger nucleases, MCT4 and BASIGIN genes in colon adenocarcinoma (LS174T) and glioblastoma (U87) human cell lines. First, we showed that homozygous loss of MCT4 dramatically sensitized cells to the MCT1 inhibitor AZD3965. Second, we demonstrated that knockout of BSG leads to a decrease in lactate transport activity of MCT1 and MCT4 by 10- and 6-fold, respectively. Consequently, cells accumulated an intracellular pool of lactic and pyruvic acids, magnified by the MCT1 inhibitor decreasing further pHi and glycolysis. As a result, we found that these glycolytic/MCT-deficient cells resumed growth by redirecting their metabolism toward OXPHOS. Third, we showed that in contrast with parental cells, BSG-null cells became highly sensitive to phenformin, an inhibitor of mitochondrial complex I. Phenformin addition to these MCT-disrupted cells in normoxic and hypoxic conditions induced a rapid drop in cellular ATP-inducing cell death by "metabolic catastrophe." Finally, xenograft analysis confirmed the deleterious tumor growth effect of MCT1/MCT4 ablation, an action enhanced by phenformin treatment. Collectively, these findings highlight that inhibition of the MCT/BSG complexes alone or in combination with phenformin provides an acute anticancer strategy to target highly glycolytic tumors. This genetic approach validates the anticancer potential of the MCT1 and MCT4 inhibitors in current development. ©2014 American Association for Cancer Research.

  12. Adenosine triphosphate levels during anaphylactic histamine release in rat mast cells in vitro. Effects of glycolytic and respiratory inhibitors

    DEFF Research Database (Denmark)

    Johansen, Torben

    1979-01-01

    The adenosine triphosphate (ATP) content of rat mast cells was studied during and after anaphylactic histamine release. The almost identical time course of ATP decrease from mast cells treated with either glycolytic or respiratory inhibitors supports the view that the ATP depletion was largely...... related to the histamine release process and not to an uncoupling of oxidative phosphorylation by an increased concentration of cytosol Ca2+. The ATP content of the cells was not restored within the 2 h of observation. No inhibition of lactate production from mast cells exposed to antigen in the presence...

  13. Deregulation and restructuring of the electric utility industry

    Energy Technology Data Exchange (ETDEWEB)

    Nixon, Hal [Utility Workers Union of America (UWUA), AFL-CIO, (United States)

    1997-12-31

    Federal and state policy makers are currently faced with the rapidly evolving issue of the restructuring and potential deregulation of the electric utility industry, a sector of the economy of huge importance through its sheer size and its impact on the daily life and livelihood of everyone. This paper describes eleven principles that must be adhered to in any restructuring of the electric industry. Adherence to the principle and positions outlined can help assure that the transition in this industry benefits all, not just a few, and that the general health and welfare of the people is protected and enhanced [Espanol] Los legisladores estatales y federales se estan enfrentando con el rapido y envolvente aspecto de la reestructuracion y desregulacion potencial de la industria electrica, un sector de la economia de enorme importancia por su tamano y su impacto en la vida diaria y los medios de vida. En esta ponencia se describen once principios y posiciones que deben ser considerados en cualquier reestructuracion de la industria electrica. El apego a los principios y posiciones comentados puede ayudar a asegurar que la transicion en esta industria deneficie a todos, no solo a unos cuantos, y que la salud general y bienestar de la gente sea protegida y mejorada

  14. The impact of organisational restructuring on perceptions of trustworthiness

    Directory of Open Access Journals (Sweden)

    Neville Bews

    2002-10-01

    Full Text Available An opportunity recently arose in a South African organisation currently undergoing drastic structural changes to assess the effects of these changes on the trust relationship between employees and those to whom these employees report. Two studies form the focus of this article. The first one was undertaken prior to the announcement of the restructuring programme. Some 13 months after the restructuring was announced and 10 months after it had commenced, a similar study focusing on trust was undertaken. In this paper the major findings of the two studies on the trust relationship in the organisation are compared. Opsomming Die geleentheid het onlangs ontstaan om die effek van hierdie veranderinge op die vertrouensverhouding tussen werknemers en diegene aan wie hulle rapporteer binne ‘n Suid-Afrikaanse organisasie wat tans drastiese strukturele veranderinge ondergaan, te beoordeel. Hierdie artikel fokus op twee studies. Die eerste een is onderneem net voor die aankondiging van die herstruktureringsprogram. Ongeveer 13 maande na die herstrukturering aangekondig is en tien maande na dit ‘n aanvang geneem het, is ‘n soortgelyke studie oor die vertrouensverhouding onderneem. In hierdie artikel word die belangrikste bevindings van die twee studies met betrekking tot die vertrouensverhouding vergelyk en die resultate bespreek.

  15. Electricity distribution industry restructuring, electrification, and competition in South Africa

    Energy Technology Data Exchange (ETDEWEB)

    Galen, P S

    1997-07-01

    This paper reviews the status of the South African electricity supply industry (ESI) and proposals for reorienting and restructuring it. South Africa has been intensely examining its ESI for more than 4 years in an effort to determine whether and how it should be restructured to best support the country`s new economic development and social upliftment goals. The debate has been spirited and inclusive of most ESI stakeholders. The demands on and expectations for the ESI are many and varied. The debate has reflected this diversity of interests and views. In essence, however, there is a consensus on what is expected of the industry, namely, to extend provision of adequate, reliable, and affordable electricity service to all citizens and segments of the economy. This means a large-scale electrification program to reach as many of the nearly 50% of households currently without electricity service as soon as possible, tariff reform to promote equity and efficiency, and the upgrading of service quality now being provided by some of the newly consolidated municipal authorities. The issues involved are how best to achieve these results within the context of the national Reconstruction and Development Program, while accounting for time and resource constraints and balancing the interests of the various parties.

  16. Restructuring, ownership and efficiency in the electricity industry

    Science.gov (United States)

    Shanefelter, Jennifer Kaiser

    The first chapter considers improvements in productive efficiency that can result from a movement from a regulated framework to one that allows for market-based incentives for industry participants. Specifically, I look at the case of restructuring in the electricity generation industry. Using data from the electricity industry, this analysis considers the total effect of restructuring on one input to the production process, labor, as reflected in employment levels, payroll per employee and aggregate establishment payroll. Using concurrent payroll and employment data from non-utility ("merchant") and utility generators in both restructured and nonrestructured states, I estimate the effect of market liberalization, comprising both new entry and state-level legislation, on employment and payroll in this industry. I find that merchant owners of divested generation assets employ significantly fewer people, but that the payroll per employee is not significantly different from what workers at utility-owned plants are paid. As a result, the new merchant owners of these plants have significantly lower aggregate payroll expenses. Decomposing the effect into a merchant effect and a divestiture effect, I find that merchant ownership is the primary driver of these results. As documented in Chapter 1, merchant power plants have lower overall payroll costs than plants owned by utilities. Employment at merchant power plants is characterized by reduced staffing levels but higher average payroll per employee. A hypothesis set forth in that paper is that merchant generators employ fewer workers at the lower end of the wage distribution, resulting in a higher average payroll per employee. The second chapter of this paper examines whether employment at nonutility power plants, that is, those that are either divested or native merchant power plants, is skewed towards more skilled labor. This chapter also considers the extent to which the difference in employment levels is the result of

  17. Closing Kynect and Restructuring Medicaid Threaten Kentucky's Health and Economy.

    Science.gov (United States)

    Wright, Charles B; Vanderford, Nathan L

    2017-08-01

    Following passage of the Patient Protection and Affordable Care Act (ACA) in the United States, the Kentucky Health Benefit Exchange, Kynect, began operating in Kentucky in October 2013. Kentucky expanded Medicaid eligibility in January 2014. Together, Kynect and Medicaid expansion provided access to affordable health care coverage to hundreds of thousands of individuals in Kentucky. However, following the Kentucky gubernatorial election in 2015, the newly inaugurated governor moved to dismantle Kynect and restructure the Medicaid expansion, jeopardizing public health gains and the state economy. As the first state to announce both the closure and restructuring of a state health insurance marketplace and Medicaid expansion, Kentucky may serve as a test case for the rest of the nation for reversal of ACA-related health policies. This article describes Kynect and the Kentucky Medicaid expansion and examines the potential short-term and long-term impacts that may occur following changes in state health policy. Furthermore, this article will offer potential strategies to ameliorate the expected negative impacts of disruption of both Kynect and the Medicaid expansion, such as the creation of a new state insurance marketplace under a new governor, the implementation of a private option, and increasing the state minimum wage for workers. Copyright © 2017 by Duke University Press.

  18. Education and Work in the context of restructuring Productive

    Directory of Open Access Journals (Sweden)

    Edson Caetano

    2009-06-01

    Full Text Available This article is a reflection on the binomial education and work, with reference to the strategy of restructuring the production process and educational programs implemented in the construction sector. The process of restructuring the productive sector is to begin in 1983, where the builders were compelled to promote changes in the organization of the work process. It is within this scenario that would fit both the strategy of outsourcing, such as literacy / professional qualification. So our focus of analysis focused on these two distinct types of initiatives, which correspond to two different times in the recent history of the company searched. For in such a way we look for to understand the changes and the implications in the conditions of work, the organization and the decurrently content of the work of the adoption of the outsourcing. This analysis is also occupied the projects of literacy kept billowing in the works, through the confrontation between the vision expressed by the construction sector's administrative and searched by employees about the meaning, importance and role of education.

  19. Restructuring of polyelectrolyte thin films in the presence of nonsolvent

    Science.gov (United States)

    Talukdar, Hrishikesh; Kundu, Sarathi

    2018-03-01

    Effects of nonsolvent (toluene) on two different polyelectrolyte thin films are studied by investigating their out-of-plane structures and in-plane surface morphologies. X-ray reflectivity analysis shows that the thicknesses of sodium poly(acrylic acid) (PAA) and poly(sodium 4-styrenesulfonate) (PSS) thin films increase if the films are kept for longer time inside toluene and nearly a linear relation is maintained between the film thickness and seasoning time. Surface topographies obtained from atomic force microscopy show that the surface morphologies and roughnesses change after dipping the films inside toluene as restructuring takes place on the surfaces of the films. Although toluene is nonsolvent for both PAA and PSS, however, restructuring of nanometer-thick polyelectrolyte is clearly visible and the effect is much more pronounced for thicker PAA and PSS films than the thinner one. Nonsolvent-induced structural relaxation of stressed structures developed under 2D confinement is the most probable reason for such structural and morphological modifications.

  20. Electricity distribution industry restructuring, electrification, and competition in South Africa

    International Nuclear Information System (INIS)

    Galen, P.S.

    1997-07-01

    This paper reviews the status of the South African electricity supply industry (ESI) and proposals for reorienting and restructuring it. South Africa has been intensely examining its ESI for more than 4 years in an effort to determine whether and how it should be restructured to best support the country's new economic development and social upliftment goals. The debate has been spirited and inclusive of most ESI stakeholders. The demands on and expectations for the ESI are many and varied. The debate has reflected this diversity of interests and views. In essence, however, there is a consensus on what is expected of the industry, namely, to extend provision of adequate, reliable, and affordable electricity service to all citizens and segments of the economy. This means a large-scale electrification program to reach as many of the nearly 50% of households currently without electricity service as soon as possible, tariff reform to promote equity and efficiency, and the upgrading of service quality now being provided by some of the newly consolidated municipal authorities. The issues involved are how best to achieve these results within the context of the national Reconstruction and Development Program, while accounting for time and resource constraints and balancing the interests of the various parties

  1. Restructuring the Energy System. Report of the Energy Commission

    International Nuclear Information System (INIS)

    1995-01-01

    The commission was instructed to examine the current energy policy programs for restructuring and developing the energy system (i.e. phasing out nuclear power and moving to renewable sources) and to analyze the needs for changes; to propose measures for ensuring an efficient electricity supply under the new conditions of a liberalized electricity market; and to present proposals for a schedule for reorganizing the energy system. The report gives a full picture of the Swedish energy system including supply, consumption, prices, environmental impacts, R and D, and international aspects. The commission concludes that one nuclear power plant can be closed during the 1990's without upsetting the power balances. Phasing out all nuclear plants by year 2010 will create problems with the price levels of electricity supply, and will conflict with the CO 2 reduction objective. The proposals for economic control measures for performing the restructuring include: An environmental bonus (or investment support) for environmentally sound electricity production financed by an electricity tax, a tax on nuclear power increasing with the age of the reactors, a reorganization fund to finance new and environmentally acceptable electricity production. Also, energy research should be allotted greater resources, in particular for new technology for electricity production. The commission points towards the possibilities for reducing energy consumption, and especially electricity consumption. Space heating should gradually move away from electric heating. Examples are given on measures for improving energy efficiency and problems with financing such measures should be studied

  2. Out-of-court restructuring versus formal bankruptcy in a non-interventionist bankruptcy setting

    NARCIS (Netherlands)

    Jostarndt, P.; Sautner, Z.

    2010-01-01

    We investigate debt restructurings in Germany for a sample of 116 financially distressed companies. About half of the firms succeed in restructuring their debt in a workout while the others file for bankruptcy. Our evidence suggests that firms which have higher leverage, owe more debt to banks, and

  3. Out-of-court restructuring versus formal bankruptcy in a non-interventionist bankruptcy setting

    NARCIS (Netherlands)

    Jostarndt, P.; Sautner, Z.

    2009-01-01

    We empirically investigate debt restructurings in Germany. Our sample consists of 116 financially distressed companies from 1997 to 2004. About half of the firms succeed in restructuring their debt in a workout while the others fail and file for bankruptcy. Our evidence suggests that firms which

  4. The Effects of Rent Restructuring on Social Housing in English Rural Areas

    Science.gov (United States)

    Walker, Bruce

    2004-01-01

    This paper discusses the impact of central government's rent restructuring policy on social housing in rural areas in England. It examines the effect that restructuring will have on the rents set by social landlords in a set of case study areas then considers some of the likely impacts on affordability and on new investment in rural social…

  5. Restructuring Student Experiences Using Tech Prep Mapping To Integrate Vocational and Academic Curriculum.

    Science.gov (United States)

    Pirozzoli, Don

    The growing emphasis on accountability and the demand for better prepared students is pressing institutions of higher education to examine restructuring. Student experiences represent an important arena for school restructuring, and a useful approach to changing student experiences is the Tech Prep Mapping (TPM) process. The TPM process requires…

  6. Industry Restructuring and Job Loss: Helping Older Workers Get Back into Employment. Research Report

    Science.gov (United States)

    Callahan, Victor J.; Bowman, Kaye

    2015-01-01

    Globalisation and increased competition bring with them many benefits for business, consumers and the economy. But they can also result in the restructuring of industries not able to compete with changing economic markets. In the past, Australia has witnessed restructuring in many high-profile businesses, especially those in its manufacturing…

  7. 77 FR 4927 - Loan Workouts and Nonaccrual Policy, and Regulatory Reporting of Troubled Debt Restructured Loans

    Science.gov (United States)

    2012-02-01

    ... Restructuring'' is as defined in generally accepted accounting principles (GAAP) and means a restructuring in... definition of a TDR under GAAP. In addition, it is important to recognize the Financial Accounting Standards... accepted accounting principles (GAAP) and the Board does not intend through this policy to change the...

  8. Changes in patient safety culture after restructuring of intensive care units: Two cross-sectional studies.

    Science.gov (United States)

    Vifladt, Anne; Simonsen, Bjoerg O; Lydersen, Stian; Farup, Per G

    2016-02-01

    Compare changes in registered nurses' perception of the patient safety culture in restructured and not restructured intensive care units during a four-year period. Two cross-sectional surveys were performed, in 2008/2009 (time 1) and 2012/2013 (time 2). During a period of 0-3 years after time 1, three of six hospitals merged their general and medical intensive care units (restructured). The other hospitals maintained their structure of the intensive care units (not restructured). Intensive care units in hospitals at one Norwegian hospital trust. The safety culture was measured with Hospital Survey on Patient Safety Culture. At times 1 and 2, 217/302 (72%) and 145/289 (50%) registered nurses participated. Restructuring was negatively associated with change in the safety culture, in particular, the dimensions of the safety culture within the unit level. The dimensions most vulnerable for restructuring were manager expectations and actions promoting safety, teamwork within hospital units and staffing. In this study, the restructuring of intensive care units was associated with a negative impact on the safety culture. When restructuring, the management should be particularly aware of changes in the safety culture dimensions manager expectations and actions promoting safety, teamwork within hospital units and staffing. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  9. Effectiveness of cognitive restructuring technique to reduce academic pracrastination of vocational high school students

    Directory of Open Access Journals (Sweden)

    Wahyu Nanda Eka Saputra

    2017-03-01

    Full Text Available Academic procrastination is a serious problem among student. Academic procrastination refers to delaying in doing assignments and preparing for examinations until the last period of examination time and submission date of assignments. To solve this problem we could use cognitive restructuring technique. The purposes of this research are to gain the description of academic procrastination decrease by implementing the cognitive restructuring technique. This research used a single subject research as the research design. The type of the single subject research which used in this research is multiple baselines. The result of this research shows that academic procrastination had significant decrease by implementation the cognitive restructuring technique. This research gives advice for two parts. First, it will be better if counselor implement the cognitive restructuring technique to decreasing academic procrastination. Second, it will be better if stakeholder of school gives support to counselor for implementation the cognitive restructuring technique to decrease academic procrastination.

  10. Effects of Interval Training-Based Glycolytic Capacity on Physical Fitness in Recreational Long-Distance Runners

    Directory of Open Access Journals (Sweden)

    Zatoń Marek

    2015-06-01

    Full Text Available Purpose. The aim of this study was to investigate the influence of 8-week-long interval training (targeting glycolytic capacity on selected markers of physical fitness in amateur long-distance runners. Methods. The study involved 17 amateur long-distance runners randomly divided into an experimental (n = 8 and control (n = 9 group. The control group performed three or four continuous training sessions per week whereas the experimental group performed two interval running training sessions and one continuous running training session. A graded treadmill exercise test and the 12-min Cooper test were performed pre- and post-training. Results. O2max and the rate of recovery increased in the experimental group. Relative oxygen uptake, minute ventilation, and heart rate speed decreased in low- (6 km/h and medium-intensity (12 km/h running. Conclusions. Both training modalities showed similar results. However, the significant differences in training volume (4-8 min interval training vs. 40-150 min continuous training indicates that the modalities targeting glycolytic capacity may be more efficient for amateur runners prepare for long-distance events.

  11. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice.

    Directory of Open Access Journals (Sweden)

    Junji Okuda

    Full Text Available BACKGROUND: Heart failure is associated with changes in cardiac energy metabolism. Glucose metabolism in particular is thought to be important in the pathogenesis of heart failure. We examined the effects of persistent overexpression of phosphoglycerate mutase 2 (Pgam2, a glycolytic enzyme, on cardiac energy metabolism and function. METHODS AND RESULTS: Transgenic mice constitutively overexpressing Pgam2 in a heart-specific manner were generated, and cardiac energy metabolism and function were analyzed. Cardiac function at rest was normal. The uptake of analogs of glucose or fatty acids and the phosphocreatine/βATP ratio at rest were normal. A comprehensive metabolomic analysis revealed an increase in the levels of a few metabolites immediately upstream and downstream of Pgam2 in the glycolytic pathway, whereas the levels of metabolites in the initial few steps of glycolysis and lactate remained unchanged. The levels of metabolites in the tricarboxylic acid (TCA cycle were altered. The capacity for respiration by isolated mitochondria in vitro was decreased, and that for the generation of reactive oxygen species (ROS in vitro was increased. Impaired cardiac function was observed in response to dobutamine. Mice developed systolic dysfunction upon pressure overload. CONCLUSIONS: Constitutive overexpression of Pgam2 modified energy metabolism and reduced stress resistance of heart in mice.

  12. Effect of aspirin and prostaglandins on the carbohydrate metabolism in albino rats.: glucose oxidation through different pathways and glycolytic enzymes

    International Nuclear Information System (INIS)

    Balasubramanian, A.; Ramakrishnan, S.

    1980-01-01

    The effect of chronic and acute doses of aspirin and prostaglandins F2α and E2 individually on the oxidation of glucose through Embden Meyerhof-TCA cycle and pentose phosphate pathways and some key glycolytic enzymes of liver were studied in male albino rats. Studies were extended to find the combined effect of PGF2α and E2 with an acute dose of aspirin. There was increased utilisation of both 1- 14 C glucose and 6- 14 C glucose on aspirin treatment. However, the metabolism through the EM-TCA pathway was more pronounced as shown by a reduced ratio of 14 CO 2 from 1- 14 C and 6- 14 C glucose. Two hepatic key glycolytic enzymes viz. hexokinase and pyruvate kinase were increased due to aspirin treatment. Withdrawal of aspirin corrected the above impaired carbohydrate metabolism in liver. Prostaglandin F2α also caused a reduction in the utilisation of 1- 14 C glucose, while PGE2 recorded an increase in the utilisation of both 1- 14 C and 6- 14 C glucose when compared to controls, indicating that different members of prostaglandins could affect metabolisms and differently. Administration of the PGs and aspirin together showed an increase in the utilisation of 6- 14 C glucose. (auth.)

  13. The Peculiar Glycolytic Pathway in Hyperthermophylic Archaea : Understanding Its Whims by Experimentation In Silico

    NARCIS (Netherlands)

    Zhang, Y.; Kouril, T.; Snoep, J.L.; Siebers, B.; Barberis, M.; Westerhoff, H.V.

    2017-01-01

    Mathematical models are key to systems biology where they typically describe the topology and dynamics of biological networks, listing biochemical entities and their relationships with one another. Some (hyper)thermophilic Archaea contain an enzyme, called non-phosphorylating

  14. Metabolomic Analysis of Oxidative and Glycolytic Skeletal Muscles by Matrix-Assisted Laser Desorption/IonizationMass Spectrometric Imaging (MALDI MSI)

    Science.gov (United States)

    Tsai, Yu-Hsuan; Garrett, Timothy J.; Carter, Christy S.; Yost, Richard A.

    2015-06-01

    Skeletal muscles are composed of heterogeneous muscle fibers that have different physiological, morphological, biochemical, and histological characteristics. In this work, skeletal muscles extensor digitorum longus, soleus, and whole gastrocnemius were analyzed by matrix-assisted laser desorption/ionization mass spectrometry to characterize small molecule metabolites of oxidative and glycolytic muscle fiber types as well as to visualize biomarker localization. Multivariate data analysis such as principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to extract significant features. Different metabolic fingerprints were observed from oxidative and glycolytic fibers. Higher abundances of biomolecules such as antioxidant anserine as well as acylcarnitines were observed in the glycolytic fibers, whereas taurine and some nucleotides were found to be localized in the oxidative fibers.

  15. The ideal restructuring of migrant families in the immigration law

    Directory of Open Access Journals (Sweden)

    Encarnación La Spina

    2013-11-01

    Full Text Available The legal configuration of kinship ties in immigration law is governed by a restrictive logic that combines a dependent and nuclear composition with mismatches in the concrete form of managing the distances, the dynamics and the times at origin and destination. The family model in immigration law has an ideal and dominant approach openly excluding other family realities in the social context. Law in an inherent tendency towards the ideal doesn’t allow a legitimate choice between autonomy and individual freedom in order to define or not the family project and own relationships. In this paper I discuss from a critical approach the inconsistencies presented by the current Spanish immigration law to restructure households in the family reunification scheme because of Law doesn’t secure a suitable degree of equality and justice.

  16. Restructuring the Vietnamese Rice Sector: Towards Increasing Sustainability

    Directory of Open Access Journals (Sweden)

    Matty Demont

    2017-02-01

    Full Text Available Although Vietnam is one of the biggest rice exporters today, there is an urgent need to restructure the sector. To guide the transition from being a quantity-focused producer to a credible supplier of quality rice, this study explores the sector’s opportunities for sustainable value chain upgrading. Data was collected through focus group discussions with farmers, stacked surveys with rice value chain stakeholders, and a participatory workshop bringing several value chain actors together. Stakeholders perceive the sector’s capability to grasp opportunities (including growing export and domestic markets to be higher than its resilience to potential threats (including more stringent food safety regulations and climate change. Three strategies are discussed for making rice value chains more sustainable; embodying sustainability in the product through certified sustainable production labels; internalizing sustainable production standards through vertical coordination (e.g., contract farming; and disembodying sustainability through book and claim certificate trading.

  17. Error in chess: the apperception-restructuring view.

    Science.gov (United States)

    Saariluoma, P

    1992-01-01

    Five protocol-analysis experiments with tactical, endgame, and strategic positions were conducted to study cognitive errors in chess players' thinking. It will be argued that chess players' errors can be only partially explained in terms of unspecified working-memory overload, because the working-memory loads caused by the solution paths are usually small. It is therefore necessary to consider apperceptive mechanisms also, as these control information intake. Subjects fail either because they are not able to see the right prototypical problem space at all, or because they fail to close them as a result of missing some crucial task-relevant cue. This makes chess players lose their "belief in the idea" and restructure, after which the apperceptive information-selection mechanisms make the finding of the solution still more unlikely.

  18. The feminization of agriculture? Economic restructuring in rural Latin America

    OpenAIRE

    Deere, Carmen Diana

    2005-01-01

    Les ménages ruraux ne cessent de diversifier leurs stratégies de création de revenus. Cette diversification, l’augmentation du nombre des membres du ménage cherchant un emploi hors de l’exploitation familiale et des femmes rurales travaillant à leur compte ou comme salariées dans l’agriculture et d’autres secteurs sont parmi les principales tendances associées à la crise économique, à la restructuration néolibérale et à la montée des taux de pauvreté dans les zones rurales d’Amérique latine. ...

  19. Restructuring of the power sector in Romania - options and perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Leca, A. [University Politechnica, Bucharest (Romania)

    1999-07-01

    Discusses the Romanian energy sector covering the situation under the Communist government and attempts at restructuring since the fall of Communism. Following the fall of Communism 1989, the power sector remained in State ownership and suffered problems such as a lack of a national energy strategy, a weak institutional framework, and the persistence of monopolistic relationships between suppliers and consumers. In 1997 a new government began a programme of energy reform aiming to, for example, improve energy efficiency, set up an institutional framework, reduce environmental impacts and re-evaluate generation capacities of existing plants and those under construction but political problems have slowed the process down. The paper suggests that privatisation is becoming the only solution to problems in the power sector caused by the deterioration of the economic and social situation in Romania. 8 figs., 1 tab.

  20. Economic restructuring in Eastern Europe and acid rain abatement strategies

    International Nuclear Information System (INIS)

    Amann, Markus; Klaassen, Ger; Schoepp, Wolfgang; Soerensen, Lene; Hordijk, Leen

    1992-01-01

    Acid rain abatement strategies in Europe are currently being discussed in view of the expiration of the Helsinki Protocol on SO 2 emission reduction. The changing energy situation in Eastern European countries is expected to have an influence on the deposition pattern in Europe. The paper presents a consistent energy scenario for Eastern European countries and compares optimal strategies to reduce SO 2 emissions. These strategies are based on runs with the RAINS model in which environmental targets have been set based on critical loads for sulphur. The analysis shows that economic restructuring and efficiency improvements in Eastern European countries, as well as in Western Europe, may result in significantly lower sulphur abatement costs. Potential assistance to Eastern Europe to guarantee desired environmental standards in Western countries should therefore focus not only on providing emission control devices but also on the success of the economic transition process. (author)

  1. Generation Gap Management in Restructured Metallurgical Enterprises in Poland

    Directory of Open Access Journals (Sweden)

    Gajdzik Bożena

    2015-09-01

    Full Text Available This paper concerns the problem of generation gap management resulting from personnel restructuring in foundries in Poland. Structural changes to steelworks led to a sharp decline in employment caused by decreased steelworks production. New hiring was limited as a part of employment rationalisation in the steelworks sector. Such personnel policy eventually led to a generation gap. Ratios between individual age groups of employees are imbalanced at steelworks: the numbers of young personnel are low and of those aged 50+ are high. This research forecasts changes in employment levels for the 2013-2019 period, aimed at closing the generation gap. The paper consists of three parts: (1 a descriptive analysis of labor market demographics in Poland’s steel industry; (2 proposed methodology for HR management model, and (3 econometric models forecasting labor demographics in Poland’s steel industry.

  2. Transition-cost issues for a restructuring US electricity industry

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    Utilities regulators can use a variety of approaches to calculate transition costs. We categorized these approaches along three dimensions. The first dimension is the use of administrative vs. market procedures to value the assets in question. Administrative approaches use analytical techniques to estimate transition costs. Market valuation relies on the purchase price of particular assets to determine their market values. The second dimension concerns when the valuation is done, either before or after the restructuring of the electricity industry. The third dimension concerns the level of detail involved in the valuation, what is often called top-down vs. bottom-up valuation. This paper discusses estimation approaches, criteria to assess estimation methods, specific approaches to estimating transition costs, factors that affect transition-cost estimates, strategies to address transition costs, who should pay transition costs, and the integration of cost recovery with competitive markets.

  3. Electricity sector restructuring in India: an environmentally beneficial policy?

    International Nuclear Information System (INIS)

    Perkins, Richard

    2005-01-01

    It has been suggested that reforms to the electricity sector in developing countries encouraging the entry of independent power producers (IPPs) are likely to result in environmental improvements similar to those recently made in a number of developed economies. The present paper evaluates this claim by examining the experience of the Indian power sector. It finds that recent investments by IPPs have reduced the pollution-intensity of electricity generation in the country. Yet they have not brought the significant gains seen in countries such as the UK, nor are they likely to in the foreseeable future. This is largely a product of the nature and context of electricity sector reform in India which is less favourable to environmentally beneficial outcomes. Accordingly, the paper concludes by suggesting that the environmental benefits of restructuring are not automatic, but depend on the existence of an enabling structural, institutional and regulatory framework

  4. THE CHALLENGES OF RAISING REVENUES AND RESTRUCTURING SUBSIDIES IN MALAYSIA

    Directory of Open Access Journals (Sweden)

    Suresh Narayanan

    2007-01-01

    Full Text Available Malaysia has run deficit budgets in all but five years since 1970 but past deficits have been managed thanks to substantial oil revenues and high domestic savings. However, the slow growth or decline of several traditional sources of revenue and the rising subsidy bill since 2007 have given pause for reflection on the traditional approach to fiscal management. In this paper, it is argued that fiscal management must not only centre around reducing non-productive expenditures and wasteful leakages but must also confront the problem of reducing and restructuring subsidies, particularly to petrol and petroleum-related products. The global dip in petroleum process has fortuitously provided the respite needed for such an exercise and should not lull policy makersinto complacency. When the economy recovers from the currentdownswing, a solid revenue raising instrument such as the value-addedtax must be introduced in order to wean the economy away from thecurrent over reliance on petroleum-based taxes.

  5. Gregarious Data Re-structuring in a Many Core Architecture

    Energy Technology Data Exchange (ETDEWEB)

    Shrestha, Sunil; Manzano Franco, Joseph B.; Marquez, Andres; Zuckerman, Stephane; Song, Shuaiwen; Gao, Guang R.

    2015-08-24

    this paper, we have developed a new methodology that takes in consideration the access patterns from a single parallel actor (e.g. a thread), as well as, the access patterns of “grouped” parallel actors that share a resource (e.g. a distributed Level 3 cache). We start with a hierarchical tile code for our target machine and apply a series of transformations at the tile level to improve data residence in a given memory hierarchy level. The contribution of this paper includes (a) collaborative data restructuring for group reuse and (b) low overhead transformation technique to improve access pattern and bring closely connected data elements together. Preliminary results in a many core architecture, Tilera TileGX, shows promising improvements over optimized OpenMP code (up to 31% increase in GFLOPS) and over our own previous work on fine grained runtimes (up to 16%) for selected kernels

  6. The organizational restructuring performative act under shareholder value management ideology

    Directory of Open Access Journals (Sweden)

    Patrícia Saltorato

    2017-04-01

    Full Text Available Purpose – This paper’s objective is to present the dynamics involving an organizational restructuring process conducted in a Brazilian subsidiary of a centenary American industrial corporation which claimed to be seeking, by means of this process, to increase the value of the company’s shares, but, which results reveal the distance between the promises and outcomes of this process, unveiling the symbolic-performative nature of such a process. Methodology – The chosen research method was the case study. The data collection involved the participant observation of a company manager (2010-2015; the conduction of non-structured interviews to the company’s managers, directors and CFO (2013-2015; documental research to the company’s internal and public sources (2010-2015; and bibliographical research to scientific papers, business press, market analysts and specialized media (2010-2015. Results – The narratives of success found in the company’s reports portray the restructuring myth as a continuous strategy, through which the company reinforces its efforts in delivering good results to shareholders. And, despite the outcomes of the case study not supporting these narratives, its performative nature legitimize the company’s commitment to the SHV management ideology towards stakeholders of the capital market, even in face of the adoption of antishareholders’ postures, such as anti-takeovers measures, the merging of the CEO and Chairman positions, its CEO perks, etc. Contributions – Paradoxically, although the researched company operates under the cold logic of finances, and under pressure to create shareholder value, it revealed to be subject to the dynamics in which the search for symbolic legitimacy plays a decisive role in maintaining its position within the hierarchy of the socially constructed field of the Management in face of other stakeholders present in the field, reinvigorating the precepts of Bourdieu’s Field Theory and

  7. Long-Term Planning in Restructured Power Systems

    International Nuclear Information System (INIS)

    Botterud, Audun

    2003-01-01

    This thesis describes the development of three decision support models for long-term investment planning in restructured power systems. The model concepts address the changing conditions for the electric power industry, with the introduction of more competitive markets, higher uncertainty and less centralised planning. Under these circumstances there is an emerging need for new planning models, also for analyses of the power system in a long-term perspective. The thesis focuses particularly on how dynamic and stochastic modelling can contribute to the improvement of decision making in a restructured power industry. We argue that the use of such modelling approaches has become more important after the introduction of competitive power markets, due to the participants' increased exposure to price fluctuations and economic risk. Our models can be applied by individual participants in the power system to evaluate investment projects for new power generation capacity. The models can also serve as a decision support tool on a regulatory level, providing analyses of the long-term performance of the power system under different regulations and market designs. In Chapter 1, we give a brief introduction to the ongoing development towards restructuring and liberalisation of the electrical power system. A discussion of the operation and organisation of restructured power systems is also provided. In Chapter 2, we look more specifically at different modelling approaches for expansion planning in electrical power systems. We also discuss how the contributions in this thesis compare to previous work in the field of decision support models for long-term planning in both regulated and competitive power systems. In Chapter 3, we develop a power market simulation model based on system dynamics. The advantages and limitations of using descriptive system dynamics models for long-term planning purposes in this context are also discussed. Chapter 4 is devoted to a novel optimisation

  8. Process restructuralization and its importance by increasing a competitiveness of an enterprise

    Directory of Open Access Journals (Sweden)

    Andrea Sujová

    2011-01-01

    Full Text Available Restructuralization in traditional understanding is considered for solution of crisis situation in an enterprise. However, it is important to emphasize that restructuralization shouldn’t be connected only with crisis situation in enterprises. In present time the restructuralization has become a tool enabling revaluation of present enterprising activities and to relocate sources towards increasing the enterprise performance. This paper is focused on substance of restructuralization based on process approach that can enable to optimalize enterprise structure and process and increase competitiveness of an enterprise. Results of research in Slovak and Czech enterprises showed that implementation of process management components by realization of strategic changes enabled to achieve the increase of business performance. Opinions of experts as also experiences of enterprises with realization of changes supported process approach as a basic condition for successful restructuralization. In paper we pay attention especially to effects of enterprise’s restructuralization with emphasis on process approach to restructuralization management which is more effective in comparison with function approach.

  9. Clean Restructuring: Design Elements for Low Carbon Wholesale Markets and Beyond

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2016-05-01

    Countries around the world are in various stages of power system reform and restructuring to more effectively meet development goals and decarbonization commitments. Changes in social dynamics, technology, business models, and environmental goals are increasing pressure for countries to consider improvements to their power systems. This brochure overviews the 21st Century Power Partnerships thought leadership report that explores the clean restructuring pathway in depth, envisions an end state, and articulates three main areas of consideration for decision makers embarking on a clean restructuring process. The report also details case studies from Germany, Denmark, and Mexico.

  10. D-glucose transport and glycolytic enzyme activities in erythrocytes of dogs, pigs, cats, horses, cattle and sheep.

    Science.gov (United States)

    Arai, T; Washizu, T; Sagara, M; Sako, T; Nigi, H; Matsumoto, H; Sasaki, M; Tomoda, I

    1995-03-01

    The activities of D-glucose transport (D-GT) and the glycolytic enzymes hexokinase (HK) and pyruvate kinase (PK), were measured in the erythrocytes of dogs, pigs, cats, horses, cattle and sheep. The erythrocytes of dogs had the highest activities of D-GT, HK and PK, significantly higher than the activities in the erythrocytes of the herbivores. The activities of D-GT and HK in cat erythrocytes were significantly lower than in those of dogs. The differences between the activities of D-GT in the erythrocytes of the different species followed the differences in activities of HK but not those in the activities of PK or in the blood glucose concentrations. It is considered that the activity of HK provides a convenient measurement of the relative rates of glucose oxidation in erythrocytes.

  11. Exposure to Glycolytic Carbon Sources Reveals a Novel Layer of Regulation for the MalT Regulon

    Directory of Open Access Journals (Sweden)

    Sylvia A. Reimann

    2011-01-01

    Full Text Available Bacteria adapt to changing environments by means of tightly coordinated regulatory circuits. The use of synthetic lethality, a genetic phenomenon in which the combination of two nonlethal mutations causes cell death, facilitates identification and study of such circuitry. In this study, we show that the E. coli ompR malTcon double mutant exhibits a synthetic lethal phenotype that is environmentally conditional. MalTcon, the constitutively active form of the maltose system regulator MalT, causes elevated expression of the outer membrane porin LamB, which leads to death in the absence of the osmoregulator OmpR. However, the presence and metabolism of glycolytic carbon sources, such as sorbitol, promotes viability and unveils a novel layer of regulation within the complex circuitry that controls maltose transport and metabolism.

  12. Twofold reduction of phosphofructokinase activity in Lactococcus lactis results in strong decreases in growth rate and in glycolytic flux

    DEFF Research Database (Denmark)

    Andersen, Heidi Winterberg; Solem, Christian; Hammer, Karin

    2001-01-01

    reduced. Surprisingly, the mutants still showed homolactic fermentation, which indicated that the limitation was different from standard glucose-limited conditions, One explanation could be that the reduced activity of phosphofructokinase resulted in the accumulation of sugar-phosphates. Indeed, when one...... kinase and lactate dehydrogenase remained closer to the wild-type level. In defined medium supplemented with glucose, the growth rate of the mutants was reduced to 57 to 70% of wild-type levels and the glycolytic flux was reduced to 62 to 76% of wild-type levels. In complex medium growth was even further...... of the mutants was starved for glucose in glucose-limited chemostat, the growth rate could gradually be increased to 195% of the growth fate observed in glucose-saturated batch culture, suggesting that phosphofructokinase does affect the concentration of upstream metabolites. The pools of glucose-6- phosphate...

  13. High-intensity intermittent exercise training with chlorella intake accelerates exercise performance and muscle glycolytic and oxidative capacity in rats.

    Science.gov (United States)

    Horii, Naoki; Hasegawa, Natsuki; Fujie, Shumpei; Uchida, Masataka; Miyamoto-Mikami, Eri; Hashimoto, Takeshi; Tabata, Izumi; Iemitsu, Motoyuki

    2017-04-01

    The purpose of this study was to investigate the effect of chronic chlorella intake alone or in combination with high-intensity intermittent exercise (HIIE) training on exercise performance and muscle glycolytic and oxidative metabolism in rats. Forty male Sprague-Dawley rats were randomly assigned to the four groups: sedentary control, chlorella intake (0.5% chlorella powder in normal feed), HIIE training, and combination of HIIE training and chlorella intake for 6 wk ( n = 10 each group). HIIE training comprised 14 repeats of a 20-s swimming session with a 10-s pause between sessions, while bearing a weight equivalent to 16% of body weight, 4 days/week. Exercise performance was tested after the interventions by measuring the maximal number of HIIE sessions that could be completed. Chlorella intake and HIIE training significantly increased the maximal number of HIIE sessions and enhanced the expression of monocarboxylate transporter (MCT)1, MCT4, and peroxisome proliferator-activated receptor γ coactivator-1α concomitantly with the activities of lactate dehydrogenase (LDH), phosphofructokinase, citrate synthase (CS), and cytochrome- c oxidase (COX) in the red region of the gastrocnemius muscle. Furthermore, the combination further augmented the increased exercise performance and the enhanced expressions and activities. By contrast, in the white region of the muscle, MCT1 expression and LDH, CS, and COX activities did not change. These results showed that compared with only chlorella intake and only HIIE training, chlorella intake combined with HIIE training has a more pronounced effect on exercise performance and muscle glycolytic and oxidative metabolism, in particular, lactate metabolism. Copyright © 2017 the American Physiological Society.

  14. European ecological networks and greenways

    DEFF Research Database (Denmark)

    Kristiansen, Ib; Jongman, Rob H.G.; Kulvik, Mart

    2004-01-01

    In the context of European integration, networks are becoming increasingly important in both social and ecological sense. Since the beginning of the 1990s, societal and scientific exchanges are being restructured as the conceptual approaches towards new nature conservation strategies have been...... renewed. Within the framework of nature conservation, the notion of an ecological network has become increasingly important. Throughout Europe, regional and national approaches are in different phases of development, which are all based on recent landscape ecological principles. Ecological networks....... This complex interaction between cultural and natural features results in quite different ways for the elaboration of ecological networks and greenways....

  15. Glycolytic enzyme activity is essential for domestic cat (Felis catus) and cheetah (Acinonyx jubatus) sperm motility and viability in a sugar-free medium.

    Science.gov (United States)

    Terrell, Kimberly A; Wildt, David E; Anthony, Nicola M; Bavister, Barry D; Leibo, S P; Penfold, Linda M; Marker, Laurie L; Crosier, Adrienne E

    2011-06-01

    We have previously reported a lack of glucose uptake in domestic cat and cheetah spermatozoa, despite observing that these cells produce lactate at rates that correlate positively with sperm function. To elucidate the role of glycolysis in felid sperm energy production, we conducted a comparative study in the domestic cat and cheetah, with the hypothesis that sperm motility and viability are maintained in both species in the absence of glycolytic metabolism and are fueled by endogenous substrates. Washed ejaculates were incubated in chemically defined medium in the presence/absence of glucose and pyruvate. A second set of ejaculates was exposed to a chemical inhibitor of either lactate dehydrogenase (sodium oxamate) or glyceraldehyde-3-phosphate dehydrogenase (alpha-chlorohydrin). Sperm function (motility and acrosomal integrity) and lactate production were assessed, and a subset of spermatozoa was assayed for intracellular glycogen. In both the cat and cheetah, sperm function was maintained without exogenous substrates and following lactate dehydrogenase inhibition. Lactate production occurred in the absence of exogenous hexoses, but only if pyruvate was present. Intracellular glycogen was not detected in spermatozoa from either species. Unexpectedly, glycolytic inhibition by alpha-chlorohydrin resulted in an immediate decline in sperm motility, particularly in the domestic cat. Collectively, our findings reveal an essential role of the glycolytic pathway in felid spermatozoa that is unrelated to hexose metabolism or lactate formation. Instead, glycolytic enzyme activity could be required for the metabolism of endogenous lipid-derived glycerol, with fatty acid oxidation providing the primary energy source in felid spermatozoa.

  16. Examining Escherichia coli glycolytic pathways, catabolite repression, and metabolite channeling using Δpfk mutants

    DEFF Research Database (Denmark)

    Hollinshead, Whitney D.; Rodriguez, Sarah; Martin, Hector Garcia

    2016-01-01

    Background: Glycolysis breakdowns glucose into essential building blocks and ATP/NAD(P)H for the cell, occupying a central role in its growth and bio-production. Among glycolytic pathways, the Entner Doudoroff pathway (EDP) is a more thermodynamically favorable pathway with fewer enzymatic steps ...

  17. The redefinition of the american and british gas industries: the regulation of the access load to the gas pipelines networks; La restructuration des industries gazieres americaine et britannique: la reglementation de la charge d'acces aux reseaux de gazoducs

    Energy Technology Data Exchange (ETDEWEB)

    David, L

    2000-10-01

    The transport and distribution networks regulation is the main stakes of the regulation reform of the gas industries. This thesis analyzes the models applied in The Usa and in the United Kingdom. The first part deals with the gas industries deregulation in these two countries, the impacts on the economy and the organization of the gas industries. The second part presents a theoretical approach of the regulation applied to the prices of the natural ags transport by gas pipelines. Regulation by the service cost price and by price cap are compared. (A.L.B.)

  18. 34 CFR 200.49 - SEA responsibilities for school improvement, corrective action, and restructuring.

    Science.gov (United States)

    2010-07-01

    ... THE ACADEMIC ACHIEVEMENT OF THE DISADVANTAGED Improving Basic Programs Operated by Local Educational..., and restructuring. (a) Transition requirements for public school choice and supplemental educational... SEA must ensure that the LEA for that school makes available supplemental educational services in...

  19. Cooperatives’ organizational restructuring, strategic attributes and performance: The case of agribusiness cooperatives in Greece

    NARCIS (Netherlands)

    Benos, T.; Kalogeras, N.; Verhees, F.; Sergaki, P.; Pennings, J.M.E.

    2016-01-01

    We develop a classification of traditional versus restructured cooperative organizational attributes based on an inductive approach. Using this classification and integrating concepts from the business literature (i.e., market and brand orientation), we hypothesize three types of relationships: (a)

  20. 24 CFR 401.100 - Which projects are eligible for a Restructuring Plan under this part?

    Science.gov (United States)

    2010-04-01

    ... HOUSING ASSISTANCE RESTRUCTURING PROGRAM (MARK-TO-MARKET) General Provisions; Eligibility § 401.100 Which... under— (i) The new construction or substantial rehabilitation program under section 8(b)(2) of the U.S...

  1. Radiation of extant cetaceans driven by restructuring of the oceans.

    Science.gov (United States)

    Steeman, Mette E; Hebsgaard, Martin B; Fordyce, R Ewan; Ho, Simon Y W; Rabosky, Daniel L; Nielsen, Rasmus; Rahbek, Carsten; Glenner, Henrik; Sørensen, Martin V; Willerslev, Eske

    2009-12-01

    The remarkable fossil record of whales and dolphins (Cetacea) has made them an exemplar of macroevolution. Although their overall adaptive transition from terrestrial to fully aquatic organisms is well known, this is not true for the radiation of modern whales. Here, we explore the diversification of extant cetaceans by constructing a robust molecular phylogeny that includes 87 of 89 extant species. The phylogeny and divergence times are derived from nuclear and mitochondrial markers, calibrated with fossils. We find that the toothed whales are monophyletic, suggesting that echolocation evolved only once early in that lineage some 36-34 Ma. The rorqual family (Balaenopteridae) is restored with the exclusion of the gray whale, suggesting that gulp feeding evolved 18-16 Ma. Delphinida, comprising all living dolphins and porpoises other than the Ganges/Indus dolphins, originated about 26 Ma; it contains the taxonomically rich delphinids, which began diversifying less than 11 Ma. We tested 2 hypothesized drivers of the extant cetacean radiation by assessing the tempo of lineage accumulation through time. We find no support for a rapid burst of speciation early in the history of extant whales, contrasting with expectations of an adaptive radiation model. However, we do find support for increased diversification rates during periods of pronounced physical restructuring of the oceans. The results imply that paleogeographic and paleoceanographic changes, such as closure of major seaways, have influenced the dynamics of radiation in extant cetaceans.

  2. Refining in the 1990's: Restructuring and resurgence

    International Nuclear Information System (INIS)

    Cobb, C.B.

    1994-01-01

    After two years of uncertainty in dealing with the 1990 Clean Air Act Amendments coupled with the shutdown of 5% of total US refining capacity, the industry is now positioning itself for continued operations throughout the remainder of the decade. However, refineries are experiencing a shift in the mode of operations to a period of more restructuring (closings, ventures, alliances, etc.) followed by a resurgence in financial performance. The purpose of this paper is to examine the current industry and highlight the reasons for industry's current plans. The authors also speculate about the strategies companies will choose to better their financial performance. Fundamentally, the characteristics of a mature domestic business remain the driving force that shape decision making. In responding to the maturing of refining, the authors suggest that refiners will change the way they conduct business over the next few years. Building on the theme of the 1993 NPRA paper, strategies will target the domestic side of the business while simultaneously shifting to a global perspective

  3. CHANGE@CERN:Task Force 5 : Restructuring the accelerator sector

    CERN Multimedia

    2002-01-01

    The last of our series on the Task Forces. PS accelerator 'We had a clear mandate, which we could approach in a logical way', explains Steve Myers, Head of SL Division and convenor of Task Force 5, 'To avoid duplication of effort in the accelerator sector through a restructuring that would lead to greater efficiency and flexibility and so release resources for the LHC.' The implementation of all their recommendations is already underway, albeit with different time scales. In 2001 the accelerator sector involved more than 900 staff members in three divisions (LHC, PS and SL) and one unit (AC), working in 141 sections within 34 groups. The first step for the Task Force was to identify major activities within the sector and to set up inter-divisional working groups to review these activities (16 in all), identifying the technologies and the numbers of staff associated with each activity. The working groups were also asked to propose ways of grouping the activities into a new more efficient organizational stru...

  4. Hospital restructuring and physician job satisfaction: an empirical study.

    Science.gov (United States)

    Mascia, Daniele; Morandi, Federica; Cicchetti, Americo

    2014-02-01

    The adoption of clinical directorates through the internal reconfiguration of hospital organizations has been one of the most widespread restructuring interventions in many Western European countries. Despite its extensive adoption, a lack of knowledge remains on the analysis of how this reorganization affects professionals' job satisfaction. This paper contributes to the debate on clinical directorates by exploring how the structural characteristics of newly adopted organizational models influence physician's job satisfaction. More than 300 physicians in 18 clinical directorates in the Italian National Health Service were surveyed regarding their overall job satisfaction following the introduction of departmental arrangements. Survey results were then linked to another survey that classified newly adopted models according to the criteria used to merge hospital wards into directorates, by recognizing "Process-integration", "Specialty-integration" and "Mixed-integration" types of directorates. Our findings show that structural aspects of change significantly influenced overall job satisfaction, and that a physician's openness to experience moderated the adoption and implementation of new clinical directorates. Specifically, results demonstrate that physicians with high openness to experience scores were more receptive to the positive impacts of change on overall job satisfaction. Implications for how these findings may facilitate organizational shifts within hospital settings are discussed. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  5. The Potential Economic Impact of Electricity Restructuring in the State of Oklahoma: Phase II Report; FINAL

    International Nuclear Information System (INIS)

    Hadley, SW

    2001-01-01

    In April 1997, the Oklahoma legislature passed a bill to restructure the state's electric industry, requiring that the generation sector be deregulated and allowing retail competition by July 2002. Details of the market structure were to be established later. Senate Bill No.220, introduced in the 2000 legislature, provided additional details on this market, but the bill did not pass. Subsequent discussions have identified the need for an objective analysis of the impact of restructuring on electricity prices and the state's economy, especially considering the experiences of other states following restructuring of their electric systems. Because of the recent experiences of other states undergoing restructuring (e.g., higher prices, greater volatility, lower reliability), concerns have been raised in states currently considering restructuring as to whether their systems are equally vulnerable. Factors such as local generation costs, transmission constraints, market concentration, and market design can all play a role in the success or failure of the market. Energy and ancillary services markets both play a role in having a well-functioning system. Customer responsiveness to market signals can enhance the flexibility of the market. The purpose of this project is to provide a model and process to evaluate the potential price and economic impacts of restructuring the Oklahoma electric industry. The goal is to provide sufficient objective analysis to the Oklahoma legislature that they may make a more informed decision on the timing and details of any future restructuring. It will also serve to inform other stakeholders on the economic issues surrounding restructuring. The project is being conducted in two phases. The Phase I report (Hadley 2001) concentrated on providing an analysis of the Oklahoma system in the near-term, using only present generation and transmission resources. This Phase II report looks further in the future, incorporating the potential of new

  6. Preparing nursing students for contemporary practice: restructuring the psychomotor skills laboratory.

    Science.gov (United States)

    Snyder, M D; Fitzloff, B M; Fiedler, R; Lambke, M R

    2000-05-01

    The restructured laboratory experience offered a safe environment that supported student experimentation with psychomotor skills and self-initiated approaches to problem solving. Restructuring psychomotor laboratory experiences with emphasis on communication and conceptualization of principles supported students to begin addressing clinical problems with flexibility, creativity, and the premise for lifelong skill acquisition. Students who have skills that extend beyond technique will inevitably be better prepared to meet the demands of health care systems and patients now and in the future.

  7. Formatting data files for repeated-measures analyses in SPSS: Using the Aggregate and Restructure procedures

    OpenAIRE

    Gyslain Giguère; Guy L. Lacroix

    2006-01-01

    In this tutorial, we demonstrate how to use the Aggregate and Restructure procedures available in SPSS (versions 11 and up) to prepare data files for repeated-measures analyses. In the first two sections of the tutorial, we briefly describe the Aggregate and Restructure procedures. In the final section, we present an example in which the data from a fictional lexical decision task are prepared for analysis using a mixed-design ANOVA. The tutorial demonstrates that the presented method is the ...

  8. Bank Restructuring, Competition, and Lending Supply: Evidence from the Spanish Banking Sector

    OpenAIRE

    Giannoccolo, Pierpaolo; Mansilla-Fernández, José Manuel

    2017-01-01

    This article analyses the effects of the bank restructuring process performed in Spain between 2010 and 2016. First, we create a unique dataset by combining information from Bankscope and the Table of Public Financial Assistance released by the Bank of Spain. Second, we investigate whether these reforms affected (i) the stability, (ii) the degree of competition, and (iii) lending and liquidity supply of the Spanish banking industry. The main results suggest that the restructuring process redu...

  9. The State of Restructuring the Electric Power Industry in the United States

    Science.gov (United States)

    Davis, Ricardo A.

    Through federal legislation, the electric power industry in the United States is encouraged to unbundle the ownership structure into separate entities for generation, transmission, and distribution of electric power. The electric power industry represents more than 7% of the U.S. gross domestic product and some of the country's major economic sectors, such as mining and chemical processing. The purpose of this qualitative, phenomenological study is to explore the phenomenon of the U.S. electric power industry restructuring from the viewpoint of those who have been at the forefront of the effort. The conceptual framework for this study is based in the Public Utilities Regulatory Policy Act (PURPA), which guided the restructuring of the U.S. electric power industry. The focus of the research questions was determining the informed perceptions and lived experience of participants. A purposive sampling approach was utilized along with a semi structured, open-ended interview with 20 participants who brought context-specific knowledge of the phenomenon. A sequential inductive process of coding, categorizing, and abstracting was performed. The findings indicate that the professionals within the industry continue to struggle with what is perceived as a restructuring effort that has come to a standstill, and doubts continue regarding the benefits or disadvantages of restructuring. The study contributes to social change by informing challenges to restructuring within the electric power industry, changes that include the potential for significant impacts to customers and especially for low income residents if subsidies are removed in a restructured environment.

  10. THE ROLE OF LOGISTICS IN ENTERPRISE RESTRUCTURING IN THE AUTOMOBILE INDUSTRY

    Directory of Open Access Journals (Sweden)

    F. A. Sabadash

    2016-01-01

    Full Text Available Formation of a market management mechanism in the Russian economy objectively requires strengthening the role of logistics in general and in the restructuring of the automotive industry in Russia in particular. In the article the main reasons that caused such an interest in the analysis is the automotive industry.Methodology. The methodological basis of this article are comparative, economic and statistical analysis methods.The purpose of this article is to reveal the key theoretical and practical issues of the role of logistics in the restructuring of the automotive industry companies, taking into account global transformations occurring in the world economy. This has allowed to specify the main issues associated with the use of logistics in the restructuring of the automotive industry in Russia.Conclusions. The following conclusions were obtained: research in the field of restructuring of the automotive industry enterprises are considered in the most general terms as "any changes to the strategic nature of the enterprise"; The second approach – the authors in their definitions focus on the restructuring of its individual aspects – goals, directions and forms of restructuring.

  11. Hospital restructuring and nursing leadership: a journey from research question to research program.

    Science.gov (United States)

    Cummings, Greta G

    2006-01-01

    The 1990s brought new fiscal realities to healthcare, leading to nursing job loss estimates in tens of thousands following widespread hospital restructuring to manage costs and improve efficiency. This research aimed at examining (a) how multiple episodes of hospital restructuring leading to layoff of nurses affected nurses who remained employed and (b) whether and how nursing leadership mitigated or intensified the negative effects of hospital restructuring on nurses. This dissertation comprised 3 empirical studies leading to 5 publications. The first study was a systematic literature review; the second and third used structural equation modeling to develop and test theoretical models addressing nursing practice environments and effects of hospital restructuring on nurses. The combined findings in this dissertation illustrate that hospital restructuring had significant negative physical/emotional health effects on nurses who remained employed. Nurses who worked for resonant (emotionally intelligent) leadership reported positive health and well-being, and opportunities to provide quality patient care. Nurses who worked for dissonant leadership reported greater negative effects of hospital restructuring. These findings led to a beginning theory of relational energy--a mechanism of mitigation whereby resonant nursing leaders invest energy into collaborative relationships with nurses, thereby positively influencing health and well-being, and, ultimately, outcomes for patients.

  12. Nodal price volatility reduction and reliability enhancement of restructured power systems considering demand-price elasticity

    International Nuclear Information System (INIS)

    Goel, L.; Wu, Qiuwei; Wang, Peng

    2008-01-01

    With the development of restructured power systems, the conventional 'same for all customers' electricity price is getting replaced by nodal prices. Electricity prices will fluctuate with time and nodes. In restructured power systems, electricity demands will interact mutually with prices. Customers may shift some of their electricity consumption from time slots of high electricity prices to those of low electricity prices if there is a commensurate price incentive. The demand side load shift will influence nodal prices in return. This interaction between demand and price can be depicted using demand-price elasticity. This paper proposes an evaluation technique incorporating the impact of the demand-price elasticity on nodal prices, system reliability and nodal reliabilities of restructured power systems. In this technique, demand and price correlations are represented using the demand-price elasticity matrix which consists of self/cross-elasticity coefficients. Nodal prices are determined using optimal power flow (OPF). The OPF and customer damage functions (CDFs) are combined in the proposed reliability evaluation technique to assess the reliability enhancement of restructured power systems considering demand-price elasticity. The IEEE reliability test system (RTS) is simulated to illustrate the developed techniques. The simulation results show that demand-price elasticity reduces the nodal price volatility and improves both the system reliability and nodal reliabilities of restructured power systems. Demand-price elasticity can therefore be utilized as a possible efficient tool to reduce price volatility and to enhance the reliability of restructured power systems. (author)

  13. Collaborative Networks as a Mechanism for Strengthening ...

    African Journals Online (AJOL)

    Collaborative Networks as a Mechanism for Strengthening Competitiveness: Small and Medium Enterprises and Non-state Actors in Tanzania as Cases. ... Coping with these challenges requires continuous restructuring and managing changes in organizations. However, only large organizations can afford to institute these ...

  14. Modeling of Aggregate Attacks on Complex Networks

    Directory of Open Access Journals (Sweden)

    F. Galindo

    2010-09-01

    Full Text Available An order factor in combinations of random and targeted attacks on modern scale free network model has been explored. Protection concepts based on timely restructuring of topologies have been discussed. Vulnerability parameter defined by investment value has been introduced, and protection financing strategies depending on node connectivity has been analyzed.

  15. Cognitive and Social Structure of the Elite Collaboration Network of Astrophysics: A Case Study on Shifting Network Structures

    Science.gov (United States)

    Heidler, Richard

    2011-01-01

    Scientific collaboration can only be understood along the epistemic and cognitive grounding of scientific disciplines. New scientific discoveries in astrophysics led to a major restructuring of the elite network of astrophysics. To study the interplay of the epistemic grounding and the social network structure of a discipline, a mixed-methods…

  16. The Rhetoric of Networked Publics. Studying Social Network Sites as Rhetorical Arenas for Political Talk

    OpenAIRE

    Vatnøy, Eirik

    2017-01-01

    As social network sites have become a central part of the modern media landscape, they have also become important arenas for political talk between citizens. The possibilities these new arenas offer for social interaction and public participation have generated what social theorists describe as a restructuring of publics into “networked publics”. Numerous studies and theories suggest how the emergence of networked publics influences political participation and democracy. Still, we...

  17. Caffeine Ingestion Increases Estimated Glycolytic Metabolism during Taekwondo Combat Simulation but Does Not Improve Performance or Parasympathetic Reactivation.

    Directory of Open Access Journals (Sweden)

    João Paulo Lopes-Silva

    Full Text Available The aim of this study was to evaluate the effect of caffeine ingestion on performance and estimated energy system contribution during simulated taekwondo combat and on post-exercise parasympathetic reactivation.Ten taekwondo athletes completed two experimental sessions separated by at least 48 hours. Athletes consumed a capsule containing either caffeine (5 mg∙kg-1 or placebo (cellulose one hour before the combat simulation (3 rounds of 2 min separated by 1 min passive recovery, in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration and rating of perceived exertion were measured before and after each round, while heart rate (HR and the estimated contribution of the oxidative (WAER, ATP-PCr (WPCR, and glycolytic (W[La-] systems were calculated during the combat simulation. Furthermore, parasympathetic reactivation after the combat simulation was evaluated through 1 taking absolute difference between the final HR observed at the end of third round and the HR recorded 60-s after (HRR60s, 2 taking the time constant of HR decay obtained by fitting the 6-min post-exercise HRR into a first-order exponential decay curve (HRRτ, or by 3 analyzing the first 30-s via logarithmic regression analysis (T30.Caffeine ingestion increased estimated glycolytic energy contribution in relation to placebo (12.5 ± 1.7 kJ and 8.9 ± 1.2 kJ, P = 0.04. However, caffeine did not improve performance as measured by attack number (CAF: 26. 7 ± 1.9; PLA: 27.3 ± 2.1, P = 0.48 or attack time (CAF: 33.8 ± 1.9 s; PLA: 36.6 ± 4.5 s, P = 0.58. Similarly, RPE (CAF: 11.7 ± 0.4 a.u.; PLA: 11.5 ± 0.3 a.u., P = 0.62, HR (CAF: 170 ± 3.5 bpm; PLA: 174.2 bpm, P = 0.12, oxidative (CAF: 109.3 ± 4.5 kJ; PLA: 107.9 kJ, P = 0.61 and ATP-PCr energy contributions (CAF: 45.3 ± 3.4 kJ; PLA: 46.8 ± 3.6 kJ, P = 0.72 during the combat simulation were unaffected. Furthermore

  18. Caffeine Ingestion Increases Estimated Glycolytic Metabolism during Taekwondo Combat Simulation but Does Not Improve Performance or Parasympathetic Reactivation.

    Science.gov (United States)

    Lopes-Silva, João Paulo; Silva Santos, Jonatas Ferreira da; Branco, Braulio Henrique Magnani; Abad, César Cavinato Cal; Oliveira, Luana Farias de; Loturco, Irineu; Franchini, Emerson

    2015-01-01

    The aim of this study was to evaluate the effect of caffeine ingestion on performance and estimated energy system contribution during simulated taekwondo combat and on post-exercise parasympathetic reactivation. Ten taekwondo athletes completed two experimental sessions separated by at least 48 hours. Athletes consumed a capsule containing either caffeine (5 mg∙kg-1) or placebo (cellulose) one hour before the combat simulation (3 rounds of 2 min separated by 1 min passive recovery), in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration and rating of perceived exertion were measured before and after each round, while heart rate (HR) and the estimated contribution of the oxidative (WAER), ATP-PCr (WPCR), and glycolytic (W[La-]) systems were calculated during the combat simulation. Furthermore, parasympathetic reactivation after the combat simulation was evaluated through 1) taking absolute difference between the final HR observed at the end of third round and the HR recorded 60-s after (HRR60s), 2) taking the time constant of HR decay obtained by fitting the 6-min post-exercise HRR into a first-order exponential decay curve (HRRτ), or by 3) analyzing the first 30-s via logarithmic regression analysis (T30). Caffeine ingestion increased estimated glycolytic energy contribution in relation to placebo (12.5 ± 1.7 kJ and 8.9 ± 1.2 kJ, P = 0.04). However, caffeine did not improve performance as measured by attack number (CAF: 26. 7 ± 1.9; PLA: 27.3 ± 2.1, P = 0.48) or attack time (CAF: 33.8 ± 1.9 s; PLA: 36.6 ± 4.5 s, P = 0.58). Similarly, RPE (CAF: 11.7 ± 0.4 a.u.; PLA: 11.5 ± 0.3 a.u., P = 0.62), HR (CAF: 170 ± 3.5 bpm; PLA: 174.2 bpm, P = 0.12), oxidative (CAF: 109.3 ± 4.5 kJ; PLA: 107.9 kJ, P = 0.61) and ATP-PCr energy contributions (CAF: 45.3 ± 3.4 kJ; PLA: 46.8 ± 3.6 kJ, P = 0.72) during the combat simulation were unaffected

  19. Caffeine Ingestion Increases Estimated Glycolytic Metabolism during Taekwondo Combat Simulation but Does Not Improve Performance or Parasympathetic Reactivation

    Science.gov (United States)

    2015-01-01

    Objectives The aim of this study was to evaluate the effect of caffeine ingestion on performance and estimated energy system contribution during simulated taekwondo combat and on post-exercise parasympathetic reactivation. Methods Ten taekwondo athletes completed two experimental sessions separated by at least 48 hours. Athletes consumed a capsule containing either caffeine (5 mg∙kg-1) or placebo (cellulose) one hour before the combat simulation (3 rounds of 2 min separated by 1 min passive recovery), in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration and rating of perceived exertion were measured before and after each round, while heart rate (HR) and the estimated contribution of the oxidative (WAER), ATP-PCr (WPCR), and glycolytic (W[La-]) systems were calculated during the combat simulation. Furthermore, parasympathetic reactivation after the combat simulation was evaluated through 1) taking absolute difference between the final HR observed at the end of third round and the HR recorded 60-s after (HRR60s), 2) taking the time constant of HR decay obtained by fitting the 6-min post-exercise HRR into a first-order exponential decay curve (HRRτ), or by 3) analyzing the first 30-s via logarithmic regression analysis (T30). Results Caffeine ingestion increased estimated glycolytic energy contribution in relation to placebo (12.5 ± 1.7 kJ and 8.9 ± 1.2 kJ, P = 0.04). However, caffeine did not improve performance as measured by attack number (CAF: 26. 7 ± 1.9; PLA: 27.3 ± 2.1, P = 0.48) or attack time (CAF: 33.8 ± 1.9 s; PLA: 36.6 ± 4.5 s, P = 0.58). Similarly, RPE (CAF: 11.7 ± 0.4 a.u.; PLA: 11.5 ± 0.3 a.u., P = 0.62), HR (CAF: 170 ± 3.5 bpm; PLA: 174.2 bpm, P = 0.12), oxidative (CAF: 109.3 ± 4.5 kJ; PLA: 107.9 kJ, P = 0.61) and ATP-PCr energy contributions (CAF: 45.3 ± 3.4 kJ; PLA: 46.8 ± 3.6 kJ, P = 0.72) during the combat simulation

  20. An Assessment of Restructured Social Studies Program in Turkey

    Directory of Open Access Journals (Sweden)

    Bülent Tarman

    2012-06-01

    Full Text Available Turkish education system has been developed taking into consideration a different perspective by setting a constructivist approach from the traditional concept of education since the 2005-2006 academic year. The restructured program has been implemented for 5 years. Research shows that many problems encountered during the implementation of the program. The encountered problems have been identified through a nationwide research study carried by EARGED in 2006 along with some other local level research studies. The purpose of this research is to determine how many of the problems, identified by EARGED’s study done in 2006, have been solved or addressed while how many of them have been still remaining. The data is collected from participants who are 6, 7 and 8th grade students who had taken social studies courses and their teachers in 16 public elementary schools located in Konya, Turkey. The collected data through this research study is compared with the findings of the EARGED’s research and a general assessment is made. asemi-structured interview schedule and a questionnaire were used to collect data from teachers and students. In qualitative analysis of the data literature which is related is scanned and descriptive analysis was conducted. Findings of this research reveals that teachers' training needs are observed mostly in areas such as “measurement and evaluation”,“knowledge about special education”, “use of information technologies” and “activity preparation” etc. and these problems still continue. At the same time the students’ problems are observed with “access to research resources”, “participation in activities or active involvement,” “teacher-student relations” and problems in such areas are still continuing

  1. Informatics requirements for a restructured competitive electric power industry

    Energy Technology Data Exchange (ETDEWEB)

    Pickle, S.; Marnay, C.; Olken, F. [eds.

    1996-08-01

    The electric power industry in the United States is undergoing a slow but nonetheless dramatic transformation. It is a transformation driven by technology, economics, and politics; one that will move the industry from its traditional mode of centralized system operations and regulated rates guaranteeing long-run cost recovery, to decentralized investment and operational decisionmaking and to customer access to true spot market prices. This transformation will revolutionize the technical, procedural, and informational requirements of the industry. A major milestone in this process occurred on December 20, 1995, when the California Public Utilities Commission (CPUC) approved its long-awaited electric utility industry restructuring decision. The decision directed the three major California investor-owned utilities to reorganize themselves by the beginning of 1998 into a supply pool, at the same time selling up to a half of their thermal generating plants. Generation will be bid into this pool and will be dispatched by an independent system operator. The dispatch could potentially involve bidders not only from California but from throughout western North America and include every conceivable generating technology and scale of operation. At the same time, large customers and aggregated customer groups will be able to contract independently for their supply and the utilities will be required to offer a real-time pricing tariff based on the pool price to all their customers, including residential. In related proceedings concerning competitive wholesale power markets, the Federal Energy Regulatory Commission (FERC) has recognized that real-time information flows between buyers and sellers are essential to efficient equitable market operation. The purpose of this meeting was to hold discussions on the information technologies that will be needed in the new, deregulated electric power industry.

  2. Organizational restructuring, government control and loss of legitimacy following an organizational crisis: the case of Israel's nonprofit human services.

    Science.gov (United States)

    Mano, Rita; Rosenberg, Dennis

    2014-01-01

    The study explores organizational restructuring following the occurrence of a crisis. Restructuring activities following an intervention are considered here to be indicators of an organization's loss of legitimacy because they have lost their independent status, a basic characteristic of nonprofit human settings. The study shows that according to the Resource Based View of organization restructuring--experienced as downsizing, neglecting and abandoning of projects--organizations are affected by (a) government intervention in decision making; (b) higher demands for accountability; and (c) higher evaluations of performance gaps. On the basis of the study of a sample of 138 Nonprofit Human Services in Israel, the results show that the higher the level of restructuring, the higher the level of legitimacy. However, organization location in metropolitan areas moderates the link between restructuring and legitimacy loss. We conclude that Israel's nonprofit human services being overly dependent on goverhment funding are more prone to restructuring and losing legitimacy following organizational crisis.

  3. Preservation of high glycolytic phenotype by establishing new acute lymphoblastic leukemia cell lines at physiologic oxygen concentration

    International Nuclear Information System (INIS)

    Sheard, Michael A.; Ghent, Matthew V.; Cabral, Daniel J.; Lee, Joanne C.; Khankaldyyan, Vazgen; Ji, Lingyun; Wu, Samuel Q.; Kang, Min H.

    2015-01-01

    Cancer cells typically exhibit increased glycolysis and decreased mitochondrial oxidative phosphorylation, and they continue to exhibit some elevation in glycolysis even under aerobic conditions. However, it is unclear whether cancer cell lines employ a high level of glycolysis comparable to that of the original cancers from which they were derived, even if their culture conditions are changed to physiologically relevant oxygen concentrations. From three childhood acute lymphoblastic leukemia (ALL) patients we established three new pairs of cell lines in both atmospheric (20%) and physiologic (bone marrow level, 5%) oxygen concentrations. Cell lines established in 20% oxygen exhibited lower proliferation, survival, expression of glycolysis genes, glucose consumption, and lactate production. Interestingly, the effects of oxygen concentration used during cell line initiation were only partially reversible when established cell cultures were switched from one oxygen concentration to another for eight weeks. These observations indicate that ALL cell lines established at atmospheric oxygen concentration can exhibit relatively low levels of glycolysis and these levels are semi-permanent, suggesting that physiologic oxygen concentrations may be needed from the time of cell line initiation to preserve the high level of glycolysis commonly exhibited by leukemias in vivo. - Highlights: • Establishing new ALL cell lines in 5% oxygen resulted in higher glycolytic expression and function. • Establishing new ALL cell lines in 5% oxygen resulted in higher proliferation and lower cell death. • The divergent metabolic phenotypes selected in 5% and 20% oxygen are semi-permanent

  4. Preservation of high glycolytic phenotype by establishing new acute lymphoblastic leukemia cell lines at physiologic oxygen concentration

    Energy Technology Data Exchange (ETDEWEB)

    Sheard, Michael A., E-mail: msheard@chla.usc.edu [Developmental Therapeutics Program, USC-CHLA Institute for Pediatric Clinical Research, Division of Hematology-Oncology, Children' s Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (United States); Ghent, Matthew V., E-mail: mattghent@gmail.com [Department of Pathology, Keck School of Medicine, University of Southern California, Health Sciences Campus, Los Angeles, CA 90089 (United States); Cabral, Daniel J., E-mail: dcabral14@gmail.com [Cancer Center and Departments of Cell Biology & Biochemistry, Pharmacology & Neuroscience, Internal Medicine and Pediatrics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430 (United States); Lee, Joanne C., E-mail: joannebarnhart@gmail.com [Cancer Center and Departments of Cell Biology & Biochemistry, Pharmacology & Neuroscience, Internal Medicine and Pediatrics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430 (United States); Khankaldyyan, Vazgen, E-mail: khangaldian@yahoo.com [Developmental Therapeutics Program, USC-CHLA Institute for Pediatric Clinical Research, Division of Hematology-Oncology, Children' s Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (United States); Ji, Lingyun, E-mail: lingyun.ji@med.usc.edu [Developmental Therapeutics Program, USC-CHLA Institute for Pediatric Clinical Research, Division of Hematology-Oncology, Children' s Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (United States); Wu, Samuel Q., E-mail: swu@chla.usc.edu [Medical Genetics, Children' s Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (United States); Kang, Min H., E-mail: min.kang@ttuhsc.edu [Cancer Center and Departments of Cell Biology & Biochemistry, Pharmacology & Neuroscience, Internal Medicine and Pediatrics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430 (United States); and others

    2015-05-15

    Cancer cells typically exhibit increased glycolysis and decreased mitochondrial oxidative phosphorylation, and they continue to exhibit some elevation in glycolysis even under aerobic conditions. However, it is unclear whether cancer cell lines employ a high level of glycolysis comparable to that of the original cancers from which they were derived, even if their culture conditions are changed to physiologically relevant oxygen concentrations. From three childhood acute lymphoblastic leukemia (ALL) patients we established three new pairs of cell lines in both atmospheric (20%) and physiologic (bone marrow level, 5%) oxygen concentrations. Cell lines established in 20% oxygen exhibited lower proliferation, survival, expression of glycolysis genes, glucose consumption, and lactate production. Interestingly, the effects of oxygen concentration used during cell line initiation were only partially reversible when established cell cultures were switched from one oxygen concentration to another for eight weeks. These observations indicate that ALL cell lines established at atmospheric oxygen concentration can exhibit relatively low levels of glycolysis and these levels are semi-permanent, suggesting that physiologic oxygen concentrations may be needed from the time of cell line initiation to preserve the high level of glycolysis commonly exhibited by leukemias in vivo. - Highlights: • Establishing new ALL cell lines in 5% oxygen resulted in higher glycolytic expression and function. • Establishing new ALL cell lines in 5% oxygen resulted in higher proliferation and lower cell death. • The divergent metabolic phenotypes selected in 5% and 20% oxygen are semi-permanent.

  5. Upregulation of glycolytic enzymes, mitochondrial dysfunction and increased cytotoxicity in glial cells treated with Alzheimer's disease plasma.

    Directory of Open Access Journals (Sweden)

    Tharusha Jayasena

    Full Text Available Alzheimer's disease (AD is a neurodegenerative disorder associated with increased oxidative stress and neuroinflammation. Markers of increased protein, lipid and nucleic acid oxidation and reduced activities of antioxidant enzymes have been reported in AD plasma. Amyloid plaques in the AD brain elicit a range of reactive inflammatory responses including complement activation and acute phase reactions, which may also be reflected in plasma. Previous studies have shown that human AD plasma may be cytotoxic to cultured cells. We investigated the effect of pooled plasma (n = 20 each from healthy controls, individuals with amnestic mild cognitive impairment (aMCI and Alzheimer's disease (AD on cultured microglial cells. AD plasma and was found to significantly decrease cell viability and increase glycolytic flux in microglia compared to plasma from healthy controls. This effect was prevented by the heat inactivation of complement. Proteomic methods and isobaric tags (iTRAQ found the expression level of complement and other acute phase proteins to be altered in MCI and AD plasma and an upregulation of key enzymes involved in the glycolysis pathway in cells exposed to AD plasma. Altered expression levels of acute phase reactants in AD plasma may alter the energy metabolism of glia.

  6. Upregulation of Glycolytic Enzymes, Mitochondrial Dysfunction and Increased Cytotoxicity in Glial Cells Treated with Alzheimer’s Disease Plasma

    Science.gov (United States)

    Jayasena, Tharusha; Poljak, Anne; Braidy, Nady; Smythe, George; Raftery, Mark; Hill, Mark; Brodaty, Henry; Trollor, Julian; Kochan, Nicole; Sachdev, Perminder

    2015-01-01

    Alzheimer’s disease (AD) is a neurodegenerative disorder associated with increased oxidative stress and neuroinflammation. Markers of increased protein, lipid and nucleic acid oxidation and reduced activities of antioxidant enzymes have been reported in AD plasma. Amyloid plaques in the AD brain elicit a range of reactive inflammatory responses including complement activation and acute phase reactions, which may also be reflected in plasma. Previous studies have shown that human AD plasma may be cytotoxic to cultured cells. We investigated the effect of pooled plasma (n = 20 each) from healthy controls, individuals with amnestic mild cognitive impairment (aMCI) and Alzheimer’s disease (AD) on cultured microglial cells. AD plasma and was found to significantly decrease cell viability and increase glycolytic flux in microglia compared to plasma from healthy controls. This effect was prevented by the heat inactivation of complement. Proteomic methods and isobaric tags (iTRAQ) found the expression level of complement and other acute phase proteins to be altered in MCI and AD plasma and an upregulation of key enzymes involved in the glycolysis pathway in cells exposed to AD plasma. Altered expression levels of acute phase reactants in AD plasma may alter the energy metabolism of glia. PMID:25785936

  7. Wnt5a Increases the Glycolytic Rate and the Activity of the Pentose Phosphate Pathway in Cortical Neurons.

    Science.gov (United States)

    Cisternas, Pedro; Salazar, Paulina; Silva-Álvarez, Carmen; Barros, L Felipe; Inestrosa, Nibaldo C

    In the last few years, several reports have proposed that Wnt signaling is a general metabolic regulator, suggesting a role for this pathway in the control of metabolic flux. Wnt signaling is critical for several neuronal functions, but little is known about the correlation between this pathway and energy metabolism. The brain has a high demand for glucose, which is mainly used for energy production. Neurons use energy for highly specific processes that require a high energy level, such as maintaining the electrical potential and synthesizing neurotransmitters. Moreover, an important metabolic impairment has been described in all neurodegenerative disorders. Despite the key role of glucose metabolism in the brain, little is known about the cellular pathways involved in regulating this process. We report here that Wnt5a induces an increase in glucose uptake and glycolytic rate and an increase in the activity of the pentose phosphate pathway; the effects of Wnt5a require the intracellular generation of nitric oxide. Our data suggest that Wnt signaling stimulates neuronal glucose metabolism, an effect that could be important for the reported neuroprotective role of Wnt signaling in neurodegenerative disorders.

  8. Wnt5a Increases the Glycolytic Rate and the Activity of the Pentose Phosphate Pathway in Cortical Neurons

    Directory of Open Access Journals (Sweden)

    Pedro Cisternas

    2016-01-01

    Full Text Available In the last few years, several reports have proposed that Wnt signaling is a general metabolic regulator, suggesting a role for this pathway in the control of metabolic flux. Wnt signaling is critical for several neuronal functions, but little is known about the correlation between this pathway and energy metabolism. The brain has a high demand for glucose, which is mainly used for energy production. Neurons use energy for highly specific processes that require a high energy level, such as maintaining the electrical potential and synthesizing neurotransmitters. Moreover, an important metabolic impairment has been described in all neurodegenerative disorders. Despite the key role of glucose metabolism in the brain, little is known about the cellular pathways involved in regulating this process. We report here that Wnt5a induces an increase in glucose uptake and glycolytic rate and an increase in the activity of the pentose phosphate pathway; the effects of Wnt5a require the intracellular generation of nitric oxide. Our data suggest that Wnt signaling stimulates neuronal glucose metabolism, an effect that could be important for the reported neuroprotective role of Wnt signaling in neurodegenerative disorders.

  9. 31P NMR saturation-transfer and 13C NMR kinetic studies of glycolytic regulation during anaerobic and aerobic glycolysis

    International Nuclear Information System (INIS)

    Campbell-Burk, S.L.; den Hollander, J.A.; Alger, J.R.; Shulman, R.G.

    1987-01-01

    31 P NMR saturation-transfer techniques have been employed in glucose-gown derepressed yeast to determine unidirectional fluxes in the upper part of the Embden-Meyerhof-Parnas pathway. The experiments were performed during anaerobic and aerobic glycolysis by saturating the ATP/sub γ/ resonances and monitoring changes in the phosphomonoester signals from glucose 6-phosphate and fructose 1,6-bisphosphate. These experiments were supplemented with 13 C NMR measurements of glucose utilization rates and 13 C NMR label distribution studies. Combined with data obtained previously from radioisotope measurement, these 31 P and 13 C NMR kinetic studies allowed estimation of the net glycolytic flow in addition to relative flows through phosphofructokinase (PFK) and Fru-1,6-P 2 ase during anaerobic and aerobic glycolysis. The 31 P NMR saturation-transfer results are consistent with previous results obtained from measurements of metabolite levels, radioisotope data, and 13 C NMR studies, providing additional support for in vivo measurement of the flows during glycolysis

  10. Restructuring of the coal industry and thermal power sector in the Commonwealth of Independent States

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-07-30

    The paper summarizes the most recent developments in the transformation of the coal and thermal power sector in Kazakhstan, Russian Federation and Ukraine to market economy conditions. It also provides statistical data concerning the dynamics of the major indicators of restructuring, such as the allocation of state subsidies, productivity growth, employment and investment. Over the last three years, the restructuring of the coal and thermal power sectors has progressed differently in the above three countries. Both sectors and especially the coal industry sector have been forced to restructure with extreme rapidity under unstable conditions, such as economic decline, absence of stable economic and fiscal laws, hyperinflation, loss of purchasing power, collapse of payment system. The transformation has been management restructuring rather than technical or reinvestment. The programmes of restructuring were mor ambitious than their realistic implementation as the lack of financial resources had, in many cases, delayed or even postponed the deadlines and objectives. The first stage has been completed in the Russian Federation, but has not yet started in Ukraine. In Kazakhstan, the coal industry has been fully privatized, mainly by foreign companies, power generation is now following along the same lines. Both sectors adapt their structure to new market requirements.

  11. Strategic restructuring for effective police system in Nigeria

    Directory of Open Access Journals (Sweden)

    Ann I. Ogbo

    2014-11-01

    Full Text Available The success of a security outfit depends on the strategies and structure of the organisation. The study aims to unravel the possible ways of positioning the Nigerian Police force for effective service delivery through strategic restructuring. Nigerian police was instituted by the colonial authors on the threshold of oppression to achieve subjection and control. Upon this pattern of operation, the Nigerian police force lost the confidence of the public. To position this agency for desired performance, several changes should be made in the strategies and structure of the force, de-emphasizing on the issues that are no longer recent problems and emphasizing on the current bane of the nation, such as corruption and insecurity. This paper adopted the mono-method qualitative approach which made use of secondary sources of data collection. Findings, revealed that the department of the Force that was responsible for information and intelligent gathering, the CID has lied dormant for long a time due to lack of adequate structure as a background that will add value to the department. Furthermore, the force was bedevilled with poor information gathering due to lack of trust and confidence in the police force, the level of motivation was found to be low, as there were no insurance policies for the Force. It is thus obvious to note that the Nigerian police force has suitable strategies that are capable of a sustainable performance, but it is challenged by lack of corresponding structure to work out the strategies. The study proposed that one DIG in addition to the twelve DIGs should be integrated to man a department with the duty of developing and maintaining good relationship with the public, and providing EFCC, ICPC and other crime related agencies with the needed force in discharging their duties. Finally, there is a need for an upward review of the reward and compensation package of the Nigerian Police Force as a way of stepping up on motivation

  12. Load As A Reliability Resource in the Restructured Electricity Market

    Energy Technology Data Exchange (ETDEWEB)

    Kueck, J.D.

    2002-06-10

    Recent electricity price spikes are painful reminders of the value that meaningful demand-side responses could bring to the restructuring US electricity system. Review of the aggregate offers made by suppliers confirms that even a modest increase in demand elasticity could dramatically reduce these extremes in price volatility. There is a strong need for dramatically increased customer participation in these markets to enhance system reliability and reduce price volatility. Indeed, allowing customers to manage their loads in response to system conditions might be thought of as the ultimate reliability resource. Most would agree that meaningful demand-side responses to price are the hallmark of a well-functioning competitive market [1]. Yet, in today's markets for electricity, little or no such response is evident. The reason is simple: customers currently do not experience directly the time-varying costs of their consumption decisions. Consequently, they have no incentive to modify these decisions in ways that might enhance system reliability or improve the efficiency of the markets in which electricity is traded. Increased customer participation is a necessary step in the evolution toward more efficient markets for electricity and ancillary services. This scoping report provides a three-part assessment of the current status of efforts to enhance the ability of customer's load to participate in competitive markets with a specific focus on the role of customer loads in enhancing electricity system reliability. First, this report considers the definitions of electricity-reliability-enhancing ancillary services (Section 2) and a preliminary assessment of the ability of customer's loads to provide these services. Second, is a review a variety of programs in which load has been called on as a system reliability resource (Section 3). These experiences, drawn from both past and current utility and ISO programs, focus on programs triggered by system

  13. Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene

    Directory of Open Access Journals (Sweden)

    Facincani Agda Paula

    2003-01-01

    Full Text Available The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, followed by cloning and expression studies of the enolase gene and determination of its activity. These studies showed that X. fastidiosa does not use the glycolytic pathway to metabolize carbohydrates, which explains the increased duplication time of this phytopatogen. Recombinant enolase was expressed as inclusion bodies and solubilized with urea (most efficient extractor, Triton X-100, and TCA. Enolase extracted from X. fastidiosa and from chicken muscle and liver is irreversibly inactivated by urea. The purification of enolase was partial and resulted in a low yield. No enzymatic activity was detected for either recombinant and native enolases, aldolase, and glyceraldehyde-3-phosphate dehydrogenase, suggesting that X. fastidiosa uses the Entner-Doudoroff pathway to produce pyruvate. Evidence is presented supporting the idea that the regulation of genes and the presence of isoforms with regulation patterns might make it difficult to understand the metabolism of carbohydrates in X. fastidiosa.

  14. Glycerophosphoglycerol, Beta-Alanine, and Pantothenic Acid as Metabolic Companions of Glycolytic Activity and Cell Migration in Breast Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Antje Hutschenreuther

    2013-11-01

    Full Text Available In cancer research, cell lines are used to explore the molecular basis of the disease as a substitute to tissue biopsies. Breast cancer in particular is a very heterogeneous type of cancer, and different subgroups of cell lines have been established according to their genomic profiles and tumor characteristics. We applied GCMS metabolite profiling to five selected breast cancer cell lines and found this heterogeneity reflected on the metabolite level as well. Metabolite profiles of MCF-7 cells belonging to the luminal gene cluster proved to be more different from those of the basal A cell line JIMT-1 and the basal B cell lines MDA-MB-231, MDA-MB-435, and MDA-MB-436 with only slight differences in the intracellular metabolite pattern. Lactate release into the cultivation medium as an indicator of glycolytic activity was correlated to the metabolite profiles and physiological characteristics of each cell line. In conclusion, pantothenic acid, beta-alanine and glycerophosphoglycerol appeared to be related to the glycolytic activity designated through high lactate release. Other physiological parameters coinciding with glycolytic activity were high glyoxalase 1 (Glo1 and lactate dehydrogenase (LDH enzyme activity as well as cell migration as an additional important characteristic contributing to the aggressiveness of tumor cells. Metabolite profiles of the cell lines are comparatively discussed with respect to known biomarkers of cancer progression.

  15. Effect of Chicory Fiber and Smoking on Quality Characteristics of Restructured Sausages

    Science.gov (United States)

    Choi, Hyun-Su; Choi, Hyung-Gyu; Choi, Yeong-Seok; Kim, Jong-Hee; Lee, Ju-Ho; Jung, Eun-Hee; Lee, Sang-Hwa; Choi, Yang-Il

    2016-01-01

    This study was conducted to investigate the effects of chicory fiber for the replacement of fat and smoking on quality characteristics of restructured sausages. Treatments were as follows; Control: Pork backfat 20%, T1: Pork backfat 10% + Chicory fiber 10%, T2: Control + Smoking, T3: T1 + Smoking. The addition of chicory fiber significantly reduced the moisture, fat, hardness and pH values, whereas the smoking treatment increased the fat, redness and pH values of restructured sausages (psausages. As a result, although the addition of chicory fiber decreased the quality characteristics of sausage, smoking treatment improved the reduced quality. Therefore, the chicory fiber and smoking treatment is helpful to develop restructured sausage products with reduced fat and compensated quality. PMID:27499674

  16. Strategy for development and restructuring of public enterprise for underground coal exploitation, Resavica

    Directory of Open Access Journals (Sweden)

    Denić Miodrag

    2014-01-01

    Full Text Available Underground coal mining in Serbia is currently in a specific and very unfavorable situation, because there is practically no strategy for future development direction of underground mines. The company is characterized by large losses in business, and therefore high debt and a chronic lack of liquidity. PE UCE 'Resavica' in restructuring has no more capital, because, unfortunately, losses are higher than capital value. It is necessary to urgently develop and adopt a Strategy of restructuring and privatization of the company, how would all economic useful resources during a certain period, be reorganized into a successful business enterprise, based on a combination of public and private investment, which would end the agony and the process of restructuring of the PE UCE 'Resavica'.

  17. The Formulation of Binding Agent Concentration on Raw Restructured Goat Meat Trimming Product

    Directory of Open Access Journals (Sweden)

    Rini Mastuti

    2012-02-01

    Full Text Available The utilization of raw materials and formulation method would affect the quality of the processed meat product. Therefore, a research on the processing method to produce a raw structured product should be conducted. The research was testing a single factor comprises of following parameters: control (without binding agent; 0.3%, 0.5% and 0.7% sodium alginat and 0.3%, 0.5% and 0.7% carragenan, respectively. The results showed that the goat meat trimming could be used as raw materials in the production of the best restructured meat using 0.5% carragenan. This raw restructured meat produced had a loss weight of 1.67%, pH value  7.89, WHC  48.345, and moisture content  75.20%.   Keywords : sodium alginat, carragenan, raw restructured goat meat trimming.

  18. Restructuring of workflows to minimise errors via stochastic model checking: An automated evolutionary approach

    DEFF Research Database (Denmark)

    Herbert, Luke Thomas; Hansen, Zaza Nadja Lee

    2016-01-01

    This article presents a framework for the automated restructuring of stochastic workflows to reduce the impact of faults. The framework allows for the modelling of workflows by means of a formalised subset of the BPMN workflow language. We extend this modelling formalism to describe faults...... and incorporate an intention preserving stochastic semantics able to model both probabilistic- and non-deterministic behaviour. Stochastic model checking techniques are employed to generate the state-space of a given workflow. Possible improvements obtained by restructuring are measured by employing the framework......'s capacity for tracking real-valued quantities associated with states and transitions of the workflow. The space of possible restructurings of a workflow are explored by means of an evolutionary algorithm, where the goals for improvement are defined in terms of optimising quantities, typically employed...

  19. Electric power industry restructuring and ROE: The case of Korea Electric Power Corporation

    International Nuclear Information System (INIS)

    Won, Gu-Hwan

    2007-01-01

    Korea's electric power industry was previously run by Korea Electric Power Corporation (KEPCO) in a natural monopoly-type structure. As economy of scale was waning in influence and management effectiveness began to increase in importance, KEPCO was divided into six subsidiaries and faced competition beginning in April 2001. This study evaluates the restructuring process for Korea's power industry and analyzes the financial outcomes based on return on equity (ROE). The results show that the ROE ratio increased. In the analysis period (1993-2004), the financial ratio showed a better performance than that before restructuring. However, productivity ratios such as productivity of capital (or gross value added to total assets) and gross value added to property, plant, and equipment decreased compared to the situation before restructuring. It is believed that the results were due to the increased financial leverage of KEPCO. For this reason, KEPCO should make further efforts to manage its debt in a systematic way

  20. Restructuring the State Electricity Boards (SEB'S) in India: the case of HSEB

    Energy Technology Data Exchange (ETDEWEB)

    Kanika, T.Bhal [Haryana State Electricity Board, HSEB, Dept. of Management (India); Abhishek, Kalra; Madhuri, Agarwal [Haryana State Electricity Board, HSEB, B. Tech, Mechanical Engineering (India)

    2001-11-01

    In India, power generation and distribution is the responsibility of the different States that have their own electricity boards through which this is done. Of late, in the wake of liberalization, many attempts have been made to reform the power sector, in the light of the poor performance of these boards. The Haryana State Electricity Board (HSEB) is the primary supplier of power in one of the States called Haryana. For quite some years, it was facing problems with revenues, low PLF etc. A review was done and counselling help sought from prominent Consultancy firms. As a result a large scale restructuring was planned. The present paper looks at the attempts of HSEB at restructuring and the consequences of the restructuring efforts. (authors)

  1. Restructuring of privatisation? - state petroleum enterprises and the global economic adjustment process

    International Nuclear Information System (INIS)

    Ndi, G.K.; Moller, L.E.

    1994-01-01

    The restructuring, and to a lesser extent the privatisation, of state petroleum enterprises in many oil-producing countries has been seen as a means of addressing the shortcomings of previous state regulation. Recent developments in the petroleum industry within the general context of the current privatisation process are examined in this chapter. Political, economic and social reasons are sought for restructuring being the preferred alternative to the privatisation of state petroleum enterprises in developing countries. The benefits, problems and obstacles to privatisation are analysed. (UK)

  2. Shell's Role in the Future European Gas Scene - Is a Major Market Restructuring Under Way?

    International Nuclear Information System (INIS)

    Segundo, Karen de

    1999-01-01

    The European gas market is being restructured by a combination of major global forces. Environmental considerations like the Kyoto Agreement favour the use of gas. But in the medium-term, supplies may be limited by low energy prices. The political drive for increased competition has manifested itself as the EU Gas Directive, which came into force in 1998. Shell believes that the European industry must progressively adapt to this restructuring. Flexible mechanisms like tradable emission permits should be used to generate investment capital for new gas supply projects. Moreover, both industry and regulators should take a positive and constructive approach towards increasing competition in the marketplace

  3. Temperature induces significant changes in both glycolytic reserve and mitochondrial spare respiratory capacity in colorectal cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Mitov, Mihail I., E-mail: m.mitov@uky.edu [Markey Cancer Center, University of Kentucky, Lexington, KY 40536 (United States); Harris, Jennifer W. [Department of Surgery, University of Kentucky, Lexington, KY 40506 (United States); Alstott, Michael C.; Zaytseva, Yekaterina Y. [Markey Cancer Center, University of Kentucky, Lexington, KY 40536 (United States); Evers, B. Mark [Markey Cancer Center, University of Kentucky, Lexington, KY 40536 (United States); Department of Surgery, University of Kentucky, Lexington, KY 40506 (United States); Butterfield, D. Allan [Markey Cancer Center, University of Kentucky, Lexington, KY 40536 (United States); Department of Chemistry, University of Kentucky, Lexington, KY 40506 (United States)

    2017-05-15

    Thermotherapy, as a method of treating cancer, has recently attracted considerable attention from basic and clinical investigators. A number of studies and clinical trials have shown that thermotherapy can be successfully used as a therapeutic approach for various cancers. However, the effects of temperature on cancer bioenergetics have not been studied in detail with a real time, microplate based, label-free detection approach. This study investigates how changes in temperature affect the bioenergetics characteristics (mitochondrial function and glycolysis) of three colorectal cancer (CRC) cell lines utilizing the Seahorse XF96 technology. Experiments were performed at 32 °C, 37 °C and 42 °C using assay medium conditions and equipment settings adjusted to produce equal oxygen and pH levels ubiquitously at the beginning of all experiments. The results suggest that temperature significantly changes multiple components of glycolytic and mitochondrial function of all cell lines tested. Under hypothermia conditions (32 °C), the extracellular acidification rates (ECAR) of CRC cells were significantly lower compared to the same basal ECAR levels measured at 37 °C. Mitochondrial stress test for SW480 cells at 37 °C vs 42 °C demonstrated increased proton leak while all other OCR components remained unchanged (similar results were detected also for the patient-derived xenograft cells Pt.93). Interestingly, the FCCP dose response at 37 °C vs 42 °C show significant shifts in profiles, suggesting that single dose FCCP experiments might not be sufficient to characterize the mitochondrial metabolic potential when comparing groups, conditions or treatments. These findings provide valuable insights for the metabolic and bioenergetic changes of CRC cells under hypo- and hyperthermia conditions that could potentially lead to development of better targeted and personalized strategies for patients undergoing combined thermotherapy with chemotherapy.

  4. Temperature induces significant changes in both glycolytic reserve and mitochondrial spare respiratory capacity in colorectal cancer cell lines

    International Nuclear Information System (INIS)

    Mitov, Mihail I.; Harris, Jennifer W.; Alstott, Michael C.; Zaytseva, Yekaterina Y.; Evers, B. Mark; Butterfield, D. Allan

    2017-01-01

    Thermotherapy, as a method of treating cancer, has recently attracted considerable attention from basic and clinical investigators. A number of studies and clinical trials have shown that thermotherapy can be successfully used as a therapeutic approach for various cancers. However, the effects of temperature on cancer bioenergetics have not been studied in detail with a real time, microplate based, label-free detection approach. This study investigates how changes in temperature affect the bioenergetics characteristics (mitochondrial function and glycolysis) of three colorectal cancer (CRC) cell lines utilizing the Seahorse XF96 technology. Experiments were performed at 32 °C, 37 °C and 42 °C using assay medium conditions and equipment settings adjusted to produce equal oxygen and pH levels ubiquitously at the beginning of all experiments. The results suggest that temperature significantly changes multiple components of glycolytic and mitochondrial function of all cell lines tested. Under hypothermia conditions (32 °C), the extracellular acidification rates (ECAR) of CRC cells were significantly lower compared to the same basal ECAR levels measured at 37 °C. Mitochondrial stress test for SW480 cells at 37 °C vs 42 °C demonstrated increased proton leak while all other OCR components remained unchanged (similar results were detected also for the patient-derived xenograft cells Pt.93). Interestingly, the FCCP dose response at 37 °C vs 42 °C show significant shifts in profiles, suggesting that single dose FCCP experiments might not be sufficient to characterize the mitochondrial metabolic potential when comparing groups, conditions or treatments. These findings provide valuable insights for the metabolic and bioenergetic changes of CRC cells under hypo- and hyperthermia conditions that could potentially lead to development of better targeted and personalized strategies for patients undergoing combined thermotherapy with chemotherapy.

  5. Evaluation of mitochondrial respiratory function in highly glycolytic glioma cells reveals low ADP phosphorylation in relation to oxidative capacity.

    Science.gov (United States)

    Rodrigues-Silva, Erika; Siqueira-Santos, Edilene S; Ruas, Juliana S; Ignarro, Raffaela S; Figueira, Tiago R; Rogério, Fábio; Castilho, Roger F

    2017-07-01

    High-grade gliomas are aggressive and intensely glycolytic tumors. In the present study, we evaluated the mitochondrial respiratory function of glioma cells (T98G and U-87MG) and fresh human glioblastoma (GBM) tissue. To this end, measurements of oxygen consumption rate (OCR) were performed under various experimental conditions. The OCR of T98G and U-87MG cells was well coupled to ADP phosphorylation based on the ratio of ATP produced per oxygen consumed of ~2.5. In agreement, the basal OCR of GBM tissue was also partially associated with ADP phosphorylation. The basal respiration of intact T98G and U-87MG cells was not limited by the supply of endogenous substrates, as indicated by the increased OCR in response to a protonophore. These cells also displayed a high affinity for oxygen, as evidenced by the values of the partial pressure of oxygen when respiration is half maximal (p 50 ). In permeabilized glioma cells, ADP-stimulated OCR was only approximately 50% of that obtained in the presence of protonophore, revealing a significant limitation in oxidative phosphorylation (OXPHOS) relative to the activity of the electron transport system (ETS). This characteristic was maintained when the cells were grown under low glucose conditions. Flux control coefficient analyses demonstrated that the impaired OXPHOS was associated with the function of both mitochondrial ATP synthase and the adenine nucleotide translocator, but not the phosphate carrier. Altogether, these data indicate that the availability and metabolism of respiratory substrates and mitochondrial ETS are preserved in T98G and U-87MG glioma cells even though these cells possess a relatively restrained OXPHOS capability.

  6. The MMPI-2-Restructured Form and the Standard MMPI-2 Clinical Scales in Relation to DSM-IV

    NARCIS (Netherlands)

    Heijden, P.T. van der; Egger, J.I.M.; Rossi, G.M.P.; Grundel, G.; Derksen, J.J.L.

    2013-01-01

    In a Dutch sample of psychiatric outpatients (N = 94), we linked the Minnesota Multiphasic Personality Inventory–2 (MMPI-2; Butcher et al., 2001) Clinical scales and MMPI-2-Restructured Form (MMPI-2-RF; Ben-Porath & Tellegen, 2008) Higher-Order (H-O) scales, Restructured Clinical (RC) scales and

  7. Restructuring Principal Preparation in Illinois: Perspectives on Implementation Successes, Challenges, and Future Outlook. Policy Research: IERC 2015-3

    Science.gov (United States)

    Klostermann, Brenda K.; Pareja, Amber Stitziel; Hart, Holly; White, Bradford R.; Huynh, Michelle Hanh

    2015-01-01

    In June 2010, the Illinois General Assembly passed Public Act 96-0903, a sweeping restructuring of the preparation of school principals and assistant principals that represented 10 years of effort from a broad coalition of stakeholders. The restructuring in Illinois was part of a movement nationwide to provide stronger training for principals in…

  8. Determining the Effects on Residential Electricity Prices and Carbon Emissions of Electricity Market Restructuring in Alberta

    Science.gov (United States)

    Jahangir, Junaid Bin

    When electricity restructuring initiatives were introduced in Alberta, and finalized with the institution of retail electricity market competition in 2001, it was argued that the changes would deliver lower electricity prices to residential consumers. However, residential electricity prices in Alberta increased dramatically in 2001, and have never returned to their pre-restructuring levels. Proponents of restructuring argue that electricity prices would have been even higher under continued regulation, citing the effect of considerably higher natural gas prices and the roles of other variables. However, many Alberta residential electricity consumers tend to attribute their higher electricity prices to factors such as market power and manipulation associated with restructuring. Since the effects of restructuring on electricity prices cannot be evaluated by simply comparing prices before and after it occurred, the main objective of this thesis is to determine what electricity prices would have been under continued regulation, and to compare them with what was actually observed. To determine these counterfactual electricity prices, a structural model of the determinants of Alberta residential electricity prices is developed, estimated for the prerestructuring period, and used to forecast (counterfactual) prices in the postrestructuring period. However, in forming these forecasts it is necessary to separately account for changes in explanatory variables that could be viewed as occurring due to the restructuring (endogenous) from those changes that would Since the effects of restructuring on electricity prices cannot be evaluated by simply comparing prices before and after it occurred, the main objective of this thesis is to determine what electricity prices would have been under continued regulation, and to compare them with what was actually observed. To determine these counterfactual electricity prices, a structural model of the determinants of Alberta residential

  9. Mobile telecommunications networks restructuring as a response to a challenging environment

    CERN Document Server

    Curwen, P

    2014-01-01

    During the past decade, no industry has grown faster than that of mobile communications, yet coverage of its operations remains scarce. This state-of-the-art book examines the evolving structure and strategic behaviour of the thirty largest operators in the mobile communications industry.

  10. Public Tourism Education and Training in Greece: A Study of the Necessity for Educational Restructuring

    Science.gov (United States)

    Diplari, Anna; Dimou, Irini

    2010-01-01

    The authors examine the Greek public tourism education and training system and its structure in order to identify whether there is a need for restructuring and development. To this end, they assess the relationship between the knowledge and skills provided and the requirements of the labour market and the needs of tourism enterprises with regard…

  11. The Theoretical Foundations of Strategic Management of Restructuring the Enterprises on the Basis of Potentials

    Directory of Open Access Journals (Sweden)

    Shpak Sergiy O.

    2017-04-01

    Full Text Available The article is aimed at developing an internally consistent system of formal strictly defined concepts of the theory of strategic management of industrial restructuring and substantiating, on this basis, appropriateness of an approach to strategic planning for restructuring, based on the monitoring and management of enterprise potentials. It has been shown that restructuring of the production sphere of industrial enterprise is a process of targeted changes in the structure of enterprise, affecting the material-technical supply, production, and marketing subsystems, which are generally managed at the strategic, tactical and operational levels. A comprehensive characterization of the current and future (of the possible structures of enterprise may be provided by its potentials. The conception of strategic management of enterprises restructuring based on the development of their potentials dictates certain requirements to the composition of tasks to be solved and the methodological tools used for this purpose, among them, priority should be given to the development of potentially promising enterprise structures and the choice of the most promising ones, as well as methods for assessment of potentials.

  12. 24 CFR 401.101 - Which owners are ineligible to request Restructuring Plans?

    Science.gov (United States)

    2010-04-01

    ... RESTRUCTURING PROGRAM (MARK-TO-MARKET) General Provisions; Eligibility § 401.101 Which owners are ineligible to...) An affiliate is debarred or suspended under 2 CFR part 2424; or (2) HUD notifies the owner that HUD... affiliate, and the grounds for the pending action are included in § 401.403(b)(2)(ii). (c) Exception for...

  13. The impact of urban growth on commuting patterns in a restructuring city : Evidence from Beijing

    NARCIS (Netherlands)

    Zhao, Pengjun; Lu, Bin; de Roo, Gert; Lu, Bo

    2011-01-01

    Our existing knowledge of the links between urban growth and commuting patterns are dominated by cases from developed countries. This paper examines the impact of urban growth on workers' commutes using the case of Beijing, which is undergoing rapid economic and spatial restructuring. The results of

  14. Beyond Common Sense in Educational Restructuring: The Issues of Content and Linkage.

    Science.gov (United States)

    Newmann, Fred M.

    Common-sense proposals for restructuring schools neglect two major issues: What content is needed to give direction to structural innovation? and How can the many factors that influence this content be linked? A discussion of the social context of U.S. education demonstrates the ways in which content and linkage problems are complicated. An agenda…

  15. "Diverse Providers" in Action: School Restructuring in Hawaii. Education Outlook. No. 8

    Science.gov (United States)

    Hess, Frederick M.; Squire, Juliet P.

    2009-01-01

    What to do about persistently low-performing schools is a pressing challenge for policymakers and educators across the nation. Schools that fail to make "adequate yearly progress" (AYP) for five consecutive years under No Child Left Behind (NCLB) must be "restructured." The 3,500 schools in the United States currently in…

  16. 24 CFR 982.504 - Voucher tenancy: Payment standard for family in restructured subsidized multifamily project.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Voucher tenancy: Payment standard... ASSISTANCE: HOUSING CHOICE VOUCHER PROGRAM Rent and Housing Assistance Payment § 982.504 Voucher tenancy: Payment standard for family in restructured subsidized multifamily project. (a) This section applies to...

  17. PRINCIPAL COURSE OF RESTRUCTURING OF MOTHER AND CHILD HEALTH PROTECTION SERVICE IN MEGAPOLIS

    Directory of Open Access Journals (Sweden)

    Yu.A. Shcherbuk

    2008-01-01

    Full Text Available The results of structural modification in pediatric and obstetric services were described in this work. This modification was directed to the increase of a quality of medical care to women and children and to the realization of priority national project «health».Key words: restructuring, children, pregnant women, infant mortality.

  18. 78 FR 41866 - Restructuring of Regulations on the Importation of Plants for Planting

    Science.gov (United States)

    2013-07-12

    ... DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service 7 CFR Parts 319 and 340 [Docket No. APHIS-2008-0011] RIN 0579-AD75 Restructuring of Regulations on the Importation of Plants for Planting AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Proposed rule; reopening of...

  19. Restructuring Teachers' Work and Trade Union Responses in England: Bargaining for Change?

    Science.gov (United States)

    Stevenson, Howard

    2007-01-01

    A key feature of current school-sector reform in England is the restructuring of teachers' work and the increased use of support staff to undertake a range of activities previously undertaken by teachers. Supporters speak of a new teacher professionalism focused on the "core task" of teaching. Critics fear deprofessionalization through a…

  20. Evaluation of Teachers for Restructured Elementary Curriculum (Grades 1 to 5)

    Science.gov (United States)

    Korkmaz, Isa

    2008-01-01

    Curriculum is a main part of education system. In order to reform educational system and policies, curriculum is taken a central consideration. Restructured curriculum means that role of teachers and students in teaching-learning atmosphere are redefined. The Ministry of National Education in Turkey began to introduce a new curriculum for…

  1. Educational Technology and the Restructuring Movement: Lessons from Research on Computers in Classrooms.

    Science.gov (United States)

    Kell, Diane; And Others

    This paper presents findings from a recently completed study of the use of computers in primary classrooms as one source of evidence concerning the role technology can play in school restructuring efforts. The sites for the study were selected by Apple Computer, Inc. in the spring of 1988 and included 43 classrooms in 10 schools in 6 large, mostly…

  2. Electric energy restructuring in the European Union: Integration, subsidiarity and the challenge of harmonization

    International Nuclear Information System (INIS)

    Serralles, Roberto J.

    2006-01-01

    In 1996, the European Union (EU) instituted Directive 96/92/EC that aims to establish a competitive, secure and transparent EU-wide internal electric energy market. The process of electricity market restructuring in the EU is being guided primarily by the principle of subsidiarity. As an organizational concept, the principle of subsidiarity allows Member States to establish their own implementation strategies and methods as a means of achieving the EU-mandated goal of electric energy restructuring. Historically, the structure of the electric energy industry in place among the Member States exhibited widely disparate organizational and functional characteristics with regard to ownership, control and regulation. Under these varied and contrasting political and economic conditions, the goal of a EU-wide harmonized electric energy market becomes very challenging. In this paper, I describe the policy mechanisms and the market conditions mandated by the EU directive aimed at liberalizing the electric energy market. I then assess the role of political culture, historical resource endowment and geographical conditions in the utility restructuring strategies of some key Member States and its effects on the overall goals of a harmonized internal electric energy market. Finally, I argue that European electric energy restructuring must be understood within the context of the political and economic milieu that spawned the individual Member States' electric energy industry

  3. Effect of Oyster Shell Calcium Powder on the Quality of Restructured Pork Ham.

    Science.gov (United States)

    Choi, Jung-Seok; Lee, Hyun-Jin; Jin, Sang-Keun; Lee, Hyun-Joo; Choi, Yang-Il

    2014-01-01

    This study was conducted to evaluate the effects of oyster shell calcium powder (OSCP) as a substitute for phosphates in curing agent, on the quality of restructured pork ham. Restructured pork ham was processed under six treatment conditions: T1 (no additives), T2 (0.3% sodium tripolyphosphate), T3 (1.5% NaCl+0.5% whey protein), T4 (1.5% NaCl+0.5% whey protein+0.15% OSCP), T5 (1.5% NaCl+0.5% whey protein+0.3% OSCP), and T6 (1.5% NaCl+0.5% whey protein+0.5% OSCP). Addition of OSCP significantly increased the ash content and pH of restructured pork ham (pham. Addition of OSCP had no effect on Hunter a and b surface color values of restructured pork ham, but did decrease the Hunter L surface color value (pham, compared with the addition of phosphates (pham.

  4. Four Rs for Urban High School Reform: Re-Envisioning, Reculturation, Restructuring, and Remoralization

    Science.gov (United States)

    Hemmings, Annette

    2012-01-01

    A framework for urban public high school reform is presented for managing site-based change through re-envisioning, reculturation, restructuring, and remoralization. The four Rs for reform framework is elucidated through a qualitative study of a low-performing urban public high school that was transformed into a new more successful school. The…

  5. Electric energy restructuring in the European Union: integration, subsidiarity and the challenge of harmonization

    International Nuclear Information System (INIS)

    Serralles, R.J.

    2006-01-01

    In 1996, the European Union (EU) instituted Directive 96/92/EC that aims to establish a competitive, secure and transparent EU-wide internal electric energy market. The process of electricity market restructuring in the EU is being guided primarily by the principle of subsidiarity. As an organizational concept, the principle of subsidiarity allows Member States to establish their own implementation strategies and methods as a means of achieving the EU-mandated goal of electric energy restructuring. Historically, the structure of the electric energy industry in place among the Member States exhibited widely disparate organizational and functional characteristics with regard to ownership, control and regulation. Under these varied and contrasting political and economic conditions, the goal of a EU-wide harmonized electric energy market becomes very challenging. In this paper, I describe the policy mechanisms and the market conditions mandated by the EU directive aimed at liberalizing the electric energy market. I then assess the role of political culture, historical resource endowment and geographical conditions in the utility restructuring strategies of some key Member States and its effects on the overall goals of a harmonized internal electric energy market. Finally, I argue that European electric energy restructuring must be understood within the context of the political and economic milieu that spawned the individual Member States' electric energy industry. (author)

  6. 75 FR 52689 - Multifamily Housing Reform and Affordability Act: Projects Eligible for a Restructuring Plan...

    Science.gov (United States)

    2010-08-27

    ... URBAN DEVELOPMENT 24 CFR Part 401 RIN 2502-AI75 Multifamily Housing Reform and Affordability Act... Multifamily and Assisted Housing Reform and Affordability Act is eligible for restructuring. Additionally, HUD... Housing Reform and Affordability Act (42 U.S.C. 1437f note) (MAHRA) introduced a Mark-to-Market program...

  7. Cooperatives’ Organizational Restructuring, Strategic Attributes and Performance: The Case of Agribusiness Cooperatives in Greece

    NARCIS (Netherlands)

    Benos, T.; Kalogeras, N.; Verhees, F.J.H.M.; Sergaki, P.; Pennings, J.M.E.

    2016-01-01

    We develop a classification of traditional vs. restructured cooperative organizational attributes based on an inductive approach. Using this classification and integrating concepts from the business literature (i.e., market and brand orientation), we hypothesize three types of relationships: a) the

  8. Effectiveness of the Sexual Attitude Restructuring Curriculum amongst Taiwanese Graduate Students

    Science.gov (United States)

    Lin, Yi-Li; Lin, Yen-Chin

    2018-01-01

    This article discusses the effectiveness of the Sexual Attitude Restructuring (SAR) curriculum in developing positive sexual attitudes amongst Taiwanese graduate students in human sexuality. Through purposive sampling, 32 graduate students in human sexuality were selected to participate in the study. Before and after providing participants with a…

  9. Local responses to global technological change – Contrasting restructuring practices in two rural communities in Austria.

    NARCIS (Netherlands)

    Fink, M.; Lang, R..; Harms, Rainer

    2013-01-01

    In this article, we investigate into local economic restructuring in rural areas that are affected by disruptive technologies. Drawing on an institutionalist framework we apply systematic theory-informed case study analysis of two rural communities in Austria and identify practices that are crucial

  10. Enhancing construction infrastructure delivery through restructuring of the Vuk’uphile Learnership Programme

    CSIR Research Space (South Africa)

    Hauptfleisch, AC

    2014-07-01

    Full Text Available The purpose of the paper is to provide an overview on review and restructuring of a construction contractor learnership programme and the quality management under the infrastructure sector of the government’s Expanded Public Works Programme (EPWP...

  11. Value Chain Restructuring, Work Organization and Labour Outcomes in Football Manufacturing in India

    DEFF Research Database (Denmark)

    Khara, Navjote; Lund-Thomsen, Peter

    2012-01-01

    this aim, the article develops a new analytical framework based upon the global value chain approach. Applying this framework to the football manufacturing industry of Jalandhar, India, the article concludes that value chain restructuring produces both new forms of work organization and highly gendered...

  12. RISE AND ACQUISITIONS FINANCIAL POTENTIAL IN THE RESTRUCTURING PROCESS OF CORPORATIONS

    Directory of Open Access Journals (Sweden)

    L. Alekseienko

    2013-03-01

    Full Text Available The basic conceptual foundations of financial potential mergers and acquisitions, as factors of corporations external growth are investigated, existing approaches to the concept of restructuring are generalized, necessary components of the absorption capacity of the corporation are defined.

  13. Diagnostic Construct Validity of MMPI-2 Restructured Form (MMPI-2-RF) Scale Scores

    Science.gov (United States)

    Sellbom, Martin; Bagby, R. Michael; Kushner, Shauna; Quilty, Lena C.; Ayearst, Lindsay E.

    2012-01-01

    In the current investigation, the authors examined the diagnostic construct validity of the "Minnesota Multiphasic Personality Inventory-2-Restructured Form" (MMPI-2-RF) in a patient sample. All participants were diagnosed via the "Structured Clinical Interview" for DSM-IV Axis I Disorders (SCID-I/P). The data set used in this…

  14. Detecting comorbid Axis-II status among inpatients using the MMPI-2 Restructured Clinical Scales

    NARCIS (Netherlands)

    Kamphuis, J.H.; Arbisi, P.A.; Ben-Porath, Y.S.; McNulty, J.L.

    2008-01-01

    This study examined the differential diagnostic utility of the MMPI-2 Restructured Clinical Scales (RCS) and Clinical Scales (CS) in detecting a complex multivariate clinical phenomenon: that is, comorbid Axis-II status in two matched samples of inpatients. Psychiatric inpatients diagnosed with

  15. Randomized Controlled Trial of Video Self-Modeling Following Speech Restructuring Treatment for Stuttering

    Science.gov (United States)

    Cream, Angela; O'Brian, Sue; Jones, Mark; Block, Susan; Harrison, Elisabeth; Lincoln, Michelle; Hewat, Sally; Packman, Ann; Menzies, Ross; Onslow, Mark

    2010-01-01

    Purpose: In this study, the authors investigated the efficacy of video self-modeling (VSM) following speech restructuring treatment to improve the maintenance of treatment effects. Method: The design was an open-plan, parallel-group, randomized controlled trial. Participants were 89 adults and adolescents who undertook intensive speech…

  16. The Restructuring and Implementation of an Effective Middle School Advisory Model

    Science.gov (United States)

    D'Amore, Ellen; Clayton, Erin

    2012-01-01

    The purpose of this action research study was to restructure and implement an effective middle school advisory model. This was conducted at a Middle School in Painesville Township, Ohio consisting of approximately 800 students. In order to meet Adequate Yearly Progress and adapt to the changes of the 21st century, a way to help the school progress…

  17. Avant le deluge: An investigation of some neglected dimensions of electricity restructuring in California

    Science.gov (United States)

    Golove, William Harry

    Chapter One of this dissertation focuses on describing the key participant groups and their principle interests in the restructuring process. The process of reaching agreement on the restructuring plan turned on each major interest group receiving satisfaction of all of its key objectives. Chapter Two explores the effective use of rhetoric in the electricity restructuring debate. Despite a lack of public involvement, the use of rhetoric appears to have been a prominent feature of the public policy process. A series of rhetorical concepts and mechanisms defined. Of these, the notions of rhetorical salience and rhetorical efficacy are most significant. The results of a field test on the impact of the particular examples of these tropes prominent in the restructuring debate on opinions regarding some of the key restructuring issues are presented. Chapter Three describes the sea change in the nature of demand side management (DSM) policy in the state, brought about by the restructuring of the electricity market. Whereas the primary goal of DSM had been resource acquisition, the new policy goal was to be market transformation. This chapter reviews the economic, and other social science theory that led to the development of demand side management programs based on a resource acquisition goal prior to restructuring and, subsequently, to a market transformation objective. Chapter Four examines the performance of the market during the period beginning in early 1998 through the first few months of 2000 at which time both the wholesale and retail markets in California were apparently functioning relatively well. The findings presented in this chapter are the result of a series of semi-structured interviews conducted with non-residential electricity customers that had chosen to receive electric service and, in some cases, value-added services from a competitive retail electricity service provider One of the key conclusions is that market rules appear to be critical to customer

  18. The effect of organisational restructuring on job satisfaction, career aspirations and stress levels of employees

    Directory of Open Access Journals (Sweden)

    E. N. Marais

    1996-06-01

    Full Text Available The field study reported here examined the effects of organisational restructuring on employees' job satisfaction, career aspirations and stress levels. Immediately after restructuring took place in the organisation, the employees affected by this advent were tested to determine whether restructuring would impact on the three variables. Eight months after restructuring in the organisation took place their job satisfaction, career aspirations and stress levels were measured again. The results of the research indicated no changes in job satisfaction levels, career aspirations or stress levels. The results provided no support for the expectation that after a time lapse of eight months the effects or organisational restructuring would diminish and that workers would experience a greater degree of job satisfaction. In addition, it would appear that the time lapse did not improve perceived career prospects or that stress levels decreased. Opsomming Die navorsing wat hier gerapporteer word/ het die effek van organisatoriese herstrukturering op werknemers se werkstevredenheid, loopbaanaspirasies en spanningsvlakke ondersoek. Onmiddellik nadat herstrukturering in die organisasie plaasgcvind het, is die werknemers wat deur die herstrukturering geraak is, getoets om vas te stel of herstrukturering 'n impak op die drie veranderlikes sou hê. Agt maande later is hul werkstevredenheid, loopbaanaspirasies en spanningsvlakke weer gemeet. Daar is gevind dat die werknemers se reaksie op organisatoriese herstrukturering onveranderd gebly het. Die resultate het geen steun verleen aan die verwagting dat die tydsverloop van agt maande die effek van organisatoriese herstrukturering sal verminder nie en dat werknemers tot 'n groter mate werkstevredenheid sal ervaar nie. Dit blyk verder dat die tydsverloop ook nie verbeterde loopbaanvooruitsigte tot gevolg gehad het of dat die werknemers se spanningsvlakke verlaag het nie.

  19. Using Brief Cognitive Restructuring and Cognitive Defusion Techniques to Cope With Negative Thoughts.

    Science.gov (United States)

    Larsson, Andreas; Hooper, Nic; Osborne, Lisa A; Bennett, Paul; McHugh, Louise

    2016-05-01

    Negative thoughts, experienced by 80% to 99% of the non-clinical population, have been linked to the development of psychopathology. The current study aimed to compare a cognitive restructuring and cognitive defusion technique for coping with a personally relevant negative thought. Over a 5-day period, participants used either a restructuring, defusion, or control strategy to manage a negative thought. Pre- and post-intervention participants reported (a) believability of the thought, (b) discomfort associated with the thought, (c) negativity associated with the thought, and (d) willingness to experience the thought. Daily online questionnaires assessing the total frequency of negative thought intrusions and their level of willingness to experience the negative thought were also used. Also, 10 positive and negative self-statements were rated on the same scales, and self-report measures of mood and psychological flexibility were completed. Findings indicated that defusion lowered believability, increased comfort and willingness to have the target thought, and increased positive affect significantly more than the control and cognitive restructuring. Within groups, cognitive restructuring also made significant gains in target thought discomfort, negativity, and "willingness to have" in the same direction as defusion but the no-instruction control did not. Negative thought frequency was reduced in the defusion group, maintained in the restructuring group, and increased in the no-instruction control group. Similar trends emerged from the secondary outcome measures, that is, the effects of the strategies on the positive and negative self-statements. The current findings support the efficacy of using defusion as a strategy for managing negative thoughts. © The Author(s) 2015.

  20. Finding the key to a better code: code team restructure to improve performance and outcomes.

    Science.gov (United States)

    Prince, Cynthia R; Hines, Elizabeth J; Chyou, Po-Huang; Heegeman, David J

    2014-09-01

    Code teams respond to acute life threatening changes in a patient's status 24 hours a day, 7 days a week. If any variable, whether a medical skill or non-medical quality, is lacking, the effectiveness of a code team's resuscitation could be hindered. To improve the overall performance of our hospital's code team, we implemented an evidence-based quality improvement restructuring plan. The code team restructure, which occurred over a 3-month period, included a defined number of code team participants, clear identification of team members and their primary responsibilities and position relative to the patient, and initiation of team training events and surprise mock codes (simulations). Team member assessments of the restructured code team and its performance were collected through self-administered electronic questionnaires. Time-to-defibrillation, defined as the time the code was called until the start of defibrillation, was measured for each code using actual time recordings from code summary sheets. Significant improvements in team member confidence in the skills specific to their role and clarity in their role's position were identified. Smaller improvements were seen in team leadership and reduction in the amount of extra talking and noise during a code. The average time-to-defibrillation during real codes decreased each year since the code team restructure. This type of code team restructure resulted in improvements in several areas that impact the functioning of the team, as well as decreased the average time-to-defibrillation, making it beneficial to many, including the team members, medical institution, and patients. © 2014 Marshfield Clinic.

  1. Science teachers' beliefs about teaching and reform: Case studies from a restructured high school

    Science.gov (United States)

    King, Elizabeth A.

    A qualitative research study of the beliefs of three science teachers about teaching and educational reform was carried out at a restructured high school belonging to the Coalition of Essential Schools (CES), a nationally prominent restructuring movement. One problem of educational reform is to sustain change in the science classroom. A new wave of reform is shifting the focus away from curriculum changes and towards professionalism of teachers empowered to restructure schools. The beliefs of the teachers are key to decisions made in the classroom. The teachers and staff of Metro High School adopted the Ten Common Principles of CES as their guide to restructuring and sustaining change. Changes included increased authority for teachers in shared decision making, increased staff time for professional development, grouping students heterogeneously, grouping students and faculty in teams for extended time periods, and organizing instruction around small group and individual student study (student-centered). The theoretical framework centers on the constructivist theory of learning, particularly Vygotsky's socio-cultural model, and Bakhtin's dialogic function of language. Nespor's belief system model was used to describe the four characteristic features of beliefs: episodic memories, alternativity, existential presumption, and evaluative loading. My research questions were: What memories of teaching have influenced the teachers? What are the teachers' beliefs about the learning environment? What are the teachers' beliefs about their students? What are the teachers' beliefs about student activities? Interviews were the primary data source for the case studies of the three teachers, with additional data from lesson plans, photo-voice, and other artifacts. The teachers shared many common beliefs including that strong peer support is necessary for reform. The teachers' beliefs allied themselves to the majority of the common principles of CES, especially personalization and

  2. Study of the geopolymer restructuration by impulse rheology

    International Nuclear Information System (INIS)

    Rouyer, J.; Frizon, F.; Poulesquen, A.

    2015-01-01

    The aim of the study is to describe the evolution of the microstructure during the setting process of the geo-polymer using an original rheological method named Optimal Fourier Rheology (OFR). The alkali activation of meta-kaolin enables physicochemical transformation from a fresh paste to a hard meso-porous matrix. Classically, oscillatory rheology technique provides viscoelastic moduli spectrum and enables to determine rheological comportment of the material under investigation. However the duration to perform a complete spectrum (more than 2.5 h) makes useless this technique in the case of changing material. The OFR technique decreases the measurement duration under 10 minutes and enables to perform several snapshots of the evolving rheological behaviour. Contrary to monochromatic iterations, here the applied stress takes the form of a chirp function which contains the full usable bandwidth. Interpretations of spectrums provide efficient access to structural evolution along the setting. Results show that the number of oligomers increases into the solution due to the dissolution of the meta-kaolin leading to a constant increase of the viscoelastic parameters until the gradual appearance of the percolating networks. The gelling time was rigorously assessed by using the Winter and Chambon criterion. A fractal percolating network is formed inside the material after a reaction time depending on the formulation parameters; corresponding fractal dimensions were established. After the gel point, the viscoelastic moduli grow rapidly until geo-polymers reach a classic viscoelastic state. Structural unit size were determined using moduli curves crossover and equalled to 2.1 nm in the case of Na geo-polymer; this value fits extremely well with value previously obtained by SAXS. Finally, the elasticity becomes constant in a large frequency range and the viscous parameter strongly decreases which means that the solid porous network is under formation. In conclusion, this

  3. LeftyA sensitive cytosolic pH regulation and glycolytic flux in Ishikawa human endometrial cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Salker, Madhuri S.; Zhou, Yuetao; Singh, Yogesh [Department of Physiology, University of Tuebingen, 72076 Tuebingen (Germany); Brosens, Jan [Division of Reproductive Health, Warwick Medical School, Clinical Sciences Research Laboratories, University Hospital, Coventry CV2 2DX (United Kingdom); Lang, Florian, E-mail: florian.lang@uni-tuebingen.de [Department of Physiology, University of Tuebingen, 72076 Tuebingen (Germany)

    2015-05-08

    Objective: LeftyA, a powerful regulator of stemness, embryonic differentiation, and reprogramming of cancer cells, counteracts cell proliferation and tumor growth. Key properties of tumor cells include enhanced glycolytic flux, which is highly sensitive to cytosolic pH and thus requires export of H{sup +} and lactate. H{sup +} extrusion is in part accomplished by Na{sup +}/H{sup +} exchangers, such as NHE1. An effect of LeftyA on transport processes has, however, never been reported. The present study thus explored whether LeftyA modifies regulation of cytosolic pH (pHi) in Ishikawa cells, a well differentiated endometrial carcinoma cell model. Methods: NHE1 transcript levels were determined by qRT-PCR, NHE1 protein abundance quantified by Western blotting, pH{sub i} estimated utilizing (2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein [BCECF] fluorescence, Na{sup +}/H{sup +} exchanger activity from Na{sup +} dependent realkalinization after an ammonium pulse, and lactate concentration in the supernatant utilizing an enzymatic assay and subsequent colorimetry. Results: A 2 h treatment with LeftyA (8 ng/ml) significantly decreased NHE1 transcript levels (by 99.6%), NHE1 protein abundance (by 71%), Na{sup +}/H{sup +} exchanger activity (by 55%), pHi (from 7.22 ± 0.02 to 7.05 ± 0.02), and lactate release (by 41%). Conclusions: LeftyA markedly down-regulates NHE1 expression, Na{sup +}/H{sup +} exchanger activity, pHi, and lactate release in Ishikawa cells. Those effects presumably contribute to cellular reprogramming and growth inhibition. - Highlights: • LeftyA, an inhibitor of tumor growth, reduces Na{sup +}/H{sup +}-exchanger activity by 55%. • LeftyA decreases NHE1 transcripts by 99.6% and NHE1 protein by 71%. • LeftyA decreases cytosolic pH from 7.22 ± 0.02 to 7.05 ± 0.02. • Cytosolic acidification by Lefty A decreases glycolysis by 41%. • Cytosolic acidification by Lefty A compromises energy production of tumor cells.

  4. Retinoblastoma treatment: impact of the glycolytic inhibitor 2-deoxy-d-glucose on molecular genomics expression in LHBETATAG retinal tumors

    Directory of Open Access Journals (Sweden)

    Piña Y

    2012-05-01

    involved in its in vitro and in vivo activity in inhibiting tumor cell growth.Keywords: retinoblastoma, hypoxia, genetic expression, glycolytic inhibitor, 2-DG

  5. Long term effect of curcumin in regulation of glycolytic pathway and angiogenesis via modulation of stress activated genes in prevention of cancer.

    Directory of Open Access Journals (Sweden)

    Laxmidhar Das

    Full Text Available Oxidative stress, an important factor in modulation of glycolytic pathway and induction of stress activated genes, is further augmented due to reduced antioxidant defense system, which promotes cancer progression via inducing angiogenesis. Curcumin, a naturally occurring chemopreventive phytochemical, is reported to inhibit carcinogenesis in various experimental animal models. However, the underlying mechanism involved in anticarcinogenic action of curcumin due to its long term effect is still to be reported because of its rapid metabolism, although metabolites are accumulated in tissues and remain for a longer time. Therefore, the long term effect of curcumin needs thorough investigation. The present study aimed to analyze the anticarcinogenic action of curcumin in liver, even after withdrawal of treatment in Dalton's lymphoma bearing mice. Oxidative stress observed during lymphoma progression reduced antioxidant enzyme activities, and induced angiogenesis as well as activation of early stress activated genes and glycolytic pathway. Curcumin treatment resulted in activation of antioxidant enzyme super oxide dismutase and down regulation of ROS level as well as activity of ROS producing enzyme NADPH:oxidase, expression of stress activated genes HIF-1α, cMyc and LDH activity towards normal level. Further, it lead to significant inhibition of angiogenesis, observed via MMPs activity, PKCα and VEGF level, as well as by matrigel plug assay. Thus findings of this study conclude that the long term effect of curcumin shows anticarcinogenic potential via induction of antioxidant defense system and inhibition of angiogenesis via down regulation of stress activated genes and glycolytic pathway in liver of lymphoma bearing mice.

  6. Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate.

    Science.gov (United States)

    Nilsson, H; Lindgren, D; Mandahl Forsberg, A; Mulder, H; Axelson, H; Johansson, M E

    2015-01-08

    Changes of cellular metabolism are an integral property of the malignant potential of most cancer cells. Already in the 1930s, Otto Warburg observed that tumor cells preferably utilize glycolysis and lactate fermentation for energy production, rather than the mitochondrial oxidative phosphorylation dominating in normal cells, a phenomenon today known as the Warburg effect. Even though many tumor types display a high degree of aerobic glycolysis, they still retain the activity of other energy-producing metabolic pathways. One exception seems to be the clear cell variant of renal cell carcinoma, ccRCC, where the activity of most other pathways than that of glycolysis has been shown to be reduced. This makes ccRCC a promising candidate for the use of glycolytic inhibitors in treatment of the disease. However, few studies have so far addressed this issue. In this report, we show a strikingly reduced mitochondrial respiratory capacity of primary human ccRCC cells, resulting in enhanced sensitivity to glycolytic inhibition by 3-Bromopyruvate (3BrPA). This effect was largely absent in established ccRCC cell lines, a finding that highlights the importance of using biologically relevant models in the search for new candidate cancer therapies. 3BrPA markedly reduced ATP production in primary ccRCC cells, followed by cell death. Our data suggest that glycolytic inhibitors such as 3BrPA, that has been shown to be well tolerated in vivo, should be further analyzed for the possible development of selective treatment strategies for patients with ccRCC.

  7. Organizational networks : Toward a cross-fertilization between practice and theory

    NARCIS (Netherlands)

    Biemans, WG

    Sweeping changes in the global marketplace have caused firms to restructure their ovganizations. In an increasing number of firms this has led to the development of a network organization. This article presents a brief survey of various disciplines that have come to use network concepts as part of

  8. Investigations into the performance of a distributed routing protocol for packet switching networks.

    OpenAIRE

    Lengerich, Anthony W.

    1982-01-01

    Approved for public release; distribution is unlimited Packet switching communication networks employ routing protocols to determine the path traversed by each packet as it passes through the network. Routing protocols which are adaptive and can restructure the packet paths in response to localized network congestion ace called "dynamic" routing protocols. Dynamic routing protocols seek to optimize the routing (provide the shortest path) for each packet in the network....

  9. Pyruvate kinase isoenzyme M2 is a glycolytic sensor differentially regulating cell proliferation, cell size and apoptotic cell death dependent on glucose supply

    International Nuclear Information System (INIS)

    Spoden, Gilles A.; Rostek, Ursula; Lechner, Stefan; Mitterberger, Maria; Mazurek, Sybille; Zwerschke, Werner

    2009-01-01

    The glycolytic key regulator pyruvate kinase M2 (M2-PK or PKM2) can switch between a highly active tetrameric and an inactive dimeric form. The transition between the two conformations regulates the glycolytic flux in tumor cells. We developed specific M2-PK-binding peptide aptamers which inhibit M2-PK, but not the 96% homologous M1-PK isoenzyme. In this study we demonstrate that, at normal blood glucose concentrations, peptide aptamer-mediated inhibition of M2-PK induces a significant decrease of the population doubling (PDL rate) and cell proliferation rate as well as an increase in cell size, whereas under glucose restriction an increase in PDL and cell proliferation rates but a decrease in cell size was observed. Moreover, M2-PK inhibition rescues cells from glucose starvation-induced apoptotic cell death by increasing the metabolic activity. These findings suggest that M2-PK is a metabolic sensor which regulates cell proliferation, cell growth and apoptotic cell death in a glucose supply-dependent manner.

  10. Refactoring the Embden–Meyerhof–Parnas Pathway as a Whole of Portable GlucoBricks for Implantation of Glycolytic Modules in Gram-Negative Bacteria

    Science.gov (United States)

    2017-01-01

    The Embden–Meyerhof–Parnas (EMP) pathway is generally considered to be the biochemical standard for glucose catabolism. Alas, its native genomic organization and the control of gene expression in Escherichia coli are both very intricate, which limits the portability of the EMP pathway to other biotechnologically important bacterial hosts that lack the route. In this work, the genes encoding all the enzymes of the linear EMP route have been individually recruited from the genome of E. coli K-12, edited in silico to remove their endogenous regulatory signals, and synthesized de novo following a standard (GlucoBrick) that enables their grouping in the form of functional modules at the user’s will. After verifying their activity in several glycolytic mutants of E. coli, the versatility of these GlucoBricks was demonstrated in quantitative physiology tests and biochemical assays carried out in Pseudomonas putida KT2440 and P. aeruginosa PAO1 as the heterologous hosts. Specific configurations of GlucoBricks were also adopted to streamline the downward circulation of carbon from hexoses to pyruvate in E. coli recombinants, thereby resulting in a 3-fold increase of poly(3-hydroxybutyrate) synthesis from glucose. Refactoring whole metabolic blocks in the fashion described in this work thus eases the engineering of biochemical processes where the optimization of carbon traffic is facilitated by the operation of the EMP pathway—which yields more ATP than other glycolytic routes such as the Entner–Doudoroff pathway. PMID:28121421

  11. A new nuclear function of the Entamoeba histolytica glycolytic enzyme enolase: the metabolic regulation of cytosine-5 methyltransferase 2 (Dnmt2 activity.

    Directory of Open Access Journals (Sweden)

    Ayala Tovy

    2010-02-01

    Full Text Available Cytosine-5 methyltransferases of the Dnmt2 family function as DNA and tRNA methyltransferases. Insight into the role and biological significance of Dnmt2 is greatly hampered by a lack of knowledge about its protein interactions. In this report, we address the subject of protein interaction by identifying enolase through a yeast two-hybrid screen as a Dnmt2-binding protein. Enolase, which is known to catalyze the conversion of 2-phosphoglycerate (2-PG to phosphoenolpyruvate (PEP, was shown to have both a cytoplasmatic and a nuclear localization in the parasite Entamoeba histolytica. We discovered that enolase acts as a Dnmt2 inhibitor. This unexpected inhibitory activity was antagonized by 2-PG, which suggests that glucose metabolism controls the non-glycolytic function of enolase. Interestingly, glucose starvation drives enolase to accumulate within the nucleus, which in turn leads to the formation of additional enolase-E.histolytica DNMT2 homolog (Ehmeth complex, and to a significant reduction of the tRNA(Asp methylation in the parasite. The crucial role of enolase as a Dnmt2 inhibitor was also demonstrated in E.histolytica expressing a nuclear localization signal (NLS-fused-enolase. These results establish enolase as the first Dnmt2 interacting protein, and highlight an unexpected role of a glycolytic enzyme in the modulation of Dnmt2 activity.

  12. Real-time garbage collection for list processing using restructured cells for increased reference counter size

    Science.gov (United States)

    Shuler, Jr., Robert L. (Inventor)

    1990-01-01

    In a list processing system, small reference counters are maintained in conjunction with memory cells for the purpose of identifying memory cells that become available for re-use. The counters are updated as references to the cells are created and destroyed, and when a counter of a cell is decremented to logical zero the cell is immediately returned to a list of free cells. In those cases where a counter must be incremented beyond the maximum value that can be represented in a small counter, the cell is restructured so that the additional reference count can be represented. The restructuring involves allocating an additional cell, distributing counter, tag, and pointer information among the two cells, and linking both cells appropriately into the existing list structure.

  13. Post-Soviet gas sector restructuring in the CIS: a political economy approach

    Energy Technology Data Exchange (ETDEWEB)

    Hirschhausen, C. von; Engerer, H. [DIW German Institute for Economic Research, Berlin (Germany). Dept. of International Economics

    1998-12-01

    This paper analyses progress and obstacles to gas sector reform in the most important CIS-Countries (Russia, Turkmenistan, Ukraine, Uzbekistan), taking a political economy perspective. This reform process is embedded in a very specific post-Soviet institutional framework stemming from the legacy of socialism. Firstly, we review the evolution of the gas sector for the period 1992-1998. The paper then identifies the post-Soviet specifies of gas sector restructuring, to which any reform strategy and technical assistance have to he adapted. We derive concrete, process-oriented policy conclusions to accelerate the reform process in a market-oriented way. The paper concludes with an evaluation of the perspectives of gas sector restructuring in this geopolitically strategic area of the world. (author)

  14. Broken dreams. Unmet expectations of investors in the Philippine electricity restructuring and privatization

    Energy Technology Data Exchange (ETDEWEB)

    Roxas, Fernando [Asian Institute of Management, 123 Paseo de Roxas, Legaspi Village, Makati City (Philippines); Santiago, Andrea [De La Salle University, Taft Avenue, Manila (Philippines)

    2010-11-15

    Over the last three decades, privatization and restructuring of electricity sectors has been a hot topic. The expectation was that prices would fall due to increased competition and that generators would become more innovative and efficient. However, the enthusiasm for deregulation wavered after events such as the debacle in California, the fall of Enron and the setbacks of Ontario. In the Philippines, comprehensive legislation was passed in 2001 but the implementation has been rather slow and controversial. This paper looked at the allegation of politicizing power rates and found a logical alternative to the perceived conspiracy angle. Based on market data, it is highly probable that depressed market rates was a result of coping mechanisms of new players trying to adjust to the restructured industry. (author)

  15. Broken dreams: Unmet expectations of investors in the Philippine electricity restructuring and privatization

    Energy Technology Data Exchange (ETDEWEB)

    Roxas, Fernando, E-mail: fyanroxas@aim.ed [Asian Institute of Management, 123 Paseo de Roxas, Legaspi Village, Makati City (Philippines); Santiago, Andrea, E-mail: ma.andrea.santiago@dlsu.edu.p [De La Salle University, Taft Avenue, Manila (Philippines)

    2010-11-15

    Over the last three decades, privatization and restructuring of electricity sectors has been a hot topic. The expectation was that prices would fall due to increased competition and that generators would become more innovative and efficient. However, the enthusiasm for deregulation wavered after events such as the debacle in California, the fall of Enron and the setbacks of Ontario. In the Philippines, comprehensive legislation was passed in 2001 but the implementation has been rather slow and controversial. This paper looked at the allegation of politicizing power rates and found a logical alternative to the perceived conspiracy angle. Based on market data, it is highly probable that depressed market rates was a result of coping mechanisms of new players trying to adjust to the restructured industry.

  16. Antioxidant Potential and Modulatory Effects of Restructured Lipids from the Amazonian Palms on Liver Cells

    Directory of Open Access Journals (Sweden)

    Andrea de Oliveira Falcão

    2017-01-01

    Full Text Available Enzymatic interesterification is used to manipulate oil and fat in order to obtain improved restructured lipids with desired technological properties. However, with raw materials containing significant amounts of bioactive compounds, the influence of this enzymatic process on the bioactivity of the final product is still not clear. Thus, the aim of this study is to evaluate the antioxidant potential and modulatory effects of two raw materials from the Amazonian area, buriti oil and murumuru fat, before and after lipase interesterification, on human hepatoma cells (HepG2. The results indicate that minor bioactive compounds naturally found in the raw materials and their antioxidant capacity are preserved after enzymatic interesterification, and that the restructured lipids modulate HepG2 endogenous antioxidant enzyme.

  17. L’anticipation des restructurations à l’épreuve du droit du travail

    OpenAIRE

    Kerbouc’h, Jean-Yves

    2012-01-01

    Les licenciements pour motif économique prononcés à l’occasion d’une restructuration d’entreprise révèlent souvent l’altération de la qualification professionnelle des salariés, qui ne parviennent ni à être adaptés ni à être reclassés. Ce n’est pourtant pas contre ce risque que le législateur a voulu agir. L’article procède à une analyse juridique des dispositifs existants, en s’appuyant sur l’exemple de deux monographies réalisées dans des entreprises en restructuration (voir article précéde...

  18. Recycling former psychiatric hospitals in New Zealand: echoes of deinstitutionalisation and restructuring.

    Science.gov (United States)

    Joseph, Alun E; Kearns, Robin A; Moon, Graham

    2009-03-01

    This paper addresses a gap in the understanding of the geography of deinstitutionalisation: the fate of closed asylums. We contend that the closure process was an exercise in both deinstitutionalisation and welfare state restructuring, and examine discourses surrounding the re-use of two former psychiatric hospitals in New Zealand-Seaview in Hokitika and Kingseat near Auckland. Drawing on an analysis of media coverage and field observation, we consider former asylums as sites of celebrity. We find the shadow of stigma to be prominent, manifested directly in suggestions that the former hospitals be used as prisons and in the partial redevelopment of one as a 'horror theme park'. Indirectly, we see stigma reflected in the physical deterioration of the asylums prior to closure. While this stigmatising is attributable in the first instance to deinstitutionalisation, the evidence suggests strongly that it was co-opted and exploited by the forces of restructuring.

  19. A review of rural health restructuring in the New South Wales.

    Science.gov (United States)

    Ritchie, D; Johnson, L

    1994-01-01

    In 1993 the delivery of rural health services in New South Wales was restructured by the establishment of 23 district health services with the aims of diverting resources from administration to clinical services and enhancing decision-making at the local level. Implementation of the restructuring in its infancy was examined by structured interviews conducted with five general managers and 15 of their second-tier appointees. The organisational structure of the districts, the managerial attributes required of senior staff and approaches to change were emphasised. Political constraints on organisational decision-making such as organisational design were acknowledged and the importance accorded to demonstrable achievement in managing change and incorporating consultative techniques was recognised.

  20. Automated evolutionary restructuring of workflows to minimise errors via stochastic model checking

    DEFF Research Database (Denmark)

    Herbert, Luke Thomas; Hansen, Zaza Nadja Lee; Jacobsen, Peter

    2014-01-01

    This paper presents a framework for the automated restructuring of workflows that allows one to minimise the impact of errors on a production workflow. The framework allows for the modelling of workflows by means of a formalised subset of the Business Process Modelling and Notation (BPMN) language......, a well-established visual language for modelling workflows in a business context. The framework’s modelling language is extended to include the tracking of real-valued quantities associated with the process (such as time, cost, temperature). In addition, this language also allows for an intention...... by means of a case study from the food industry. Through this case study we explore the extent to which the risk of production faults can be reduced and the impact of these can be minimised, primarily through restructuring of the production workflows. This approach is fully automated and only the modelling...

  1. False assumptions about psychopathology, hysteria and the MMPI-2 Restructured Clinical Scales.

    Science.gov (United States)

    Gordon, Robert M

    2006-06-01

    The MMPI-2 Restructured Clinical scales rest on the common behavioral assumption that consistent items can be added to assess all psychopathologies. This may be the case for some unitary symptoms such as anxiety or anger, but not for complex diagnostic conditions such as Hysteria, Post Traumatic Stress Disorder, and Borderline Personality Disorder. These are better understood with a psychodynamic formulation. Psychodynamic theory assumes that internal conflicts and contradictions are a significant feature of many psychopathologies. For example, the new MMPI-2 Restructured Clinical scales eliminated a measure of hysteria. The RC3 Cynicism scale is not an improvement over the MMPI-2 Hysteria scale, as the new scale serves as an example of a failure of the behaviorism to account for complex psychopathology. Making scales more internally consistent and distinct from each other has not produced more external validity and useful measures for many of the psychopathologies found in clinical practice.

  2. An Empirical Analysis of a Dominant Firm's Market Power in a Restructured Electricity Market, A Case Study of Colorado

    National Research Council Canada - National Science Library

    Sweetser, Wilmer

    1998-01-01

    ...., there are 32 states where one firm owns at least 40% of the existing generation. As these states consider electric restructuring, analysis of the market power that a dominant firm can exercise could become increasingly important...

  3. THE INDONESIAN BANK CRISIS AND RESTRUCTURING: LESSONS AND IMPLICATIONS FOR OTHER DEVELOPING COUNTRIES

    OpenAIRE

    Mari PANGESTU

    2003-01-01

    Drawing on the Indonesian experience in 1997, this paper demonstrates the complexity of managing banking crises deals. It aims at deriving conclusions from this experience for other developing countries facing similar problems. The paper also refers to comparative experiences of other countries, in particular Malaysia and Thailand, examines the IMF programme and reveals its shortcomings. The key question is to what extent the ineffectiveness and slow progress of bank restructuring, corporate ...

  4. Examination of Changes in Infection Rates in a Restructured Anaesthesia Intensive Care Unit: A Retrospective Study.

    Science.gov (United States)

    Deniz, Ahmet; Erhan, Ömer Lütfi; Bayar, Mustafa Kemal; Karatepe, Ümit; Demirel, İsmail

    2017-12-01

    This retrospective study aimed to evaluate the effect of a restructured anaesthesia intensive care unit (ICU) on changes in infection rates and infections. Organisational restructuring was done in the anaesthesia ICU of Firat University Hospital after it was relocated on 14 March 2012. This study was designed to investigate the effect of restructuring on infection rates through a comparison of periods encompassing one year before relocation and one year after relocation. Nosocomial infections were diagnosed according to modified Centers for Disease Control and Prevention (CDC) criteria. In total, 406 patients who were over 18 years old and admitted to the ICU were included; they were hospitalised for 48 h or longer and had non-infectious diseases according to physical examination, laboratory and culture results on admission. The data of 214 patients (Group A) and 192 patients (Group B) were examined. Parameters such as age, gender, primary diagnosis and mean GCS score at admission and mean duration of hospitalisation showed no effect on the rates of infection, but rates of total infection (41.1% vs. 25%), urinary (18.7% vs. 10.4%) and VIP (32.7% vs. 14.6%) were detected in Groups A and B. Statistically significant differences were found for the causative pathogens Pseudomonas (15.4% vs. 6.8%), Acinetobacter (18.2% vs. 12%) and Escherichia (8.9% vs. 2.1%); the mean duration of mechanical ventilation (15.01±16.681 vs. 12.22±17.595) and discharge with improvement (31.8% vs. 44.3%). We detected that restructuring (such as acclimatization, educated staff, hepa filter) caused a significant decline in infection rates. Because ICU staff may be a major cause of infection, we believe that providing education and conducting effective surveillance programs will be the most important factors for reducing infection rates.

  5. After more than a decade of restructuring; what have we learned, and where are we heading

    International Nuclear Information System (INIS)

    Lowrie, W.G.

    1996-01-01

    The paper relates to the current state and future development of the global petroleum industry. The topic of this paper looks to the future, but it requires a review of the recent past. Specifically, the author examines, after a decade of restructuring and renewing, the experience obtained and the industrial heading. In this regard, the author mainly refers to the experiences obtained at the Amoco Corporation. 3 figs

  6. After more than a decade of restructuring; what have we learned, and where are we heading

    Energy Technology Data Exchange (ETDEWEB)

    Lowrie, W.G. [Amoco Corp./Environmental Protection Agency, Yorktown, VA (United States). Pollution Prevention Project

    1996-12-31

    The paper relates to the current state and future development of the global petroleum industry. The topic of this paper looks to the future, but it requires a review of the recent past. Specifically, the author examines, after a decade of restructuring and renewing, the experience obtained and the industrial heading. In this regard, the author mainly refers to the experiences obtained at the Amoco Corporation. 3 figs.

  7. Development of market strategies of metallurgical enterrprises after restructuring of steel industry

    Directory of Open Access Journals (Sweden)

    B. Gajdzik

    2014-01-01

    Full Text Available Before metallurgical enterprises started implementation of marketing activities they had to go through restructuring processes which included all areas of their market activities. Privatised metallurgical enterprises after economic transformation gradually implemented marketing to their business activities. The article presents notions connected with development of marketing strategies from the period of last 20 years. The range of analysis includes categories corresponding with instruments of mix marketing (4P − product, price, place, promotion.

  8. Restructuring, Ownership and Efficiency: The Case of Labor in Electricity Generation

    OpenAIRE

    Shanefelter, Jennifer Kaiser

    2007-01-01

    This analysis considers improvements in productive efficiency that can result from a movement from a regulated framework to one that allows for market-based incentives for industry participants. Specifically, I look at the case of restructuring in the electricity generation industry. As numerous industries and economies have undergone this sort of transition to varying degrees, it is instructive to assess the performance of market-based incentives relative to what was observed under tighter r...

  9. Global Change and China’s Clusters: The Restructuring of Guangzhou’s Textile District.

    Directory of Open Access Journals (Sweden)

    Schröder, Friederike

    2010-03-01

    Full Text Available Economic globalization has increasingly exposed nations, regions, and cities to strong local competition and inter-city rivalry. This is also the case in the Pearl River Delta (PRD of South China. As a highly dynamic gro-wing mega-urban region, it has a longstanding reputation as China’s economic powerhouse and as the “fac-tory of the world”. Once a pioneering area of market economy reforms, the PRD nowadays faces fierce com-petition with other major metropolitan areas such as Beijing-Hebei-Metropolis and the Yangtze Delta Region, including Shanghai. This calls for constant adaptation of urban development strategies, which nowadays progressively aim at economic restructuring from labor-intensive manufacturing towards knowledge-intensive and service-oriented industries. The ascent along the value chain is particularly apparent within economic clusters, which are a long-established feature of China’s local economy. However, despite the restructuring of the economy, there is still scope for an “upgrading” of existing clusters even when they seem to represent low value-added economic sectors. The Zhongda textile cluster in Guangz-hou is a fascinating showcase example for this phenomenon. Not only is the textile manufacturing and trading sector re-establishing its role in the city, but it has even proven possible to conduct the restructuring process in situ in a densely-populated and completely built-up area. This paper will focus on the restructuring process as it took place over time in both economic and spatial terms. In the process, it will also reveal how global and local forces interplay in shaping the physical as well as the economic re-development of this cluster.

  10. Cooperatives’ Organizational Restructuring, Strategic Attributes and Performance: The Case of Agribusiness Cooperatives in Greece

    OpenAIRE

    Benos, T.; Kalogeras, N.; Verhees, F.J.H.M.; Sergaki, P.; Pennings, J.M.E.

    2016-01-01

    We develop a classification of traditional vs. restructured cooperative organizational attributes based on an inductive approach. Using this classification and integrating concepts from the business literature (i.e., market and brand orientation), we hypothesize three types of relationships: a) the influence of organizational attributes, i.e., ownership, control and cost/benefit allocation, on organizational performance; b) the influence of strategic, i.e., market and brand orientation, attri...

  11. UTILITY ADVANCED TURBINE SYSTEMS (ATS) TECHNOLOGY READINESS TESTING PHASE 3 RESTRUCTURED (3R)

    Energy Technology Data Exchange (ETDEWEB)

    Unknown

    2000-03-17

    This scope document defines the work scope for accomplishing the design of the GE MS7001H and MS9001H (7H and 9H) combined-cycle power systems under the original ATS Phase 3 DOE Cooperative Agreement No. DE-FC21-95MC31176, and incorporates changes in scope required to convert Phase 3 to the ''restructured'' Phase 3R as defined in Amendment A012 to the Cooperative Agreement.

  12. NEW BUSINESS MODELS, THE RESTRUCTURE OF COMPETITION AND IMPLICATIONS ON BUSINESS MANAGEMENT AND TRADE STATISTICS

    OpenAIRE

    SERGHEI MARGULESCU; RICHARD POSPISIL

    2014-01-01

    Economists and managers should be aware today of the fact that some concepts and economic mechanisms need to be reconsidered in the context of the new aspects of globalization. The appearance of new types of business and new trade patterns on the international stage has led to the restructure of worldwide competition. “Traditional” competition between companies is replaced, in the context of the “globally integrated businesses” and the new division of labour based on the “functional” speciali...

  13. Negociation and Transaction in a context of restructuring. The role of actors: trust and uncertainy

    OpenAIRE

    Robert, Jocelyne

    2006-01-01

    In the current context of globalization and industrial restructuring, we find it interesting to analyse a negotiation about a re-opening of a production site within a multinational enterprise. This negotiation between trade unions and management representatives was strategic for the revival of production activity and retention of 10,000 direct and indirect jobs. It allows us to illustrate the “structuration’ theory of Giddens (1990) insofar as we cannot ignore neither the importance of struct...

  14. New Restructure of Transmitted Sequences for CP-based LS Channel Estimation Method in OFDM System

    OpenAIRE

    Wang-Xing Zhao; Zhang-Xin Chen; Qun Wan

    2012-01-01

    This study proceeded to investigate a study and a signal processing on channel estimation problem of OFDM system in wireless communication area. We gave an optimization stretching into total transmitted sequences restructure which aimed to improve Cyclic-prefix Least Square (CPLS) channel estimation method proposed in this paper. By contrast to conventional Training Sequences (TS) methods especially frequency TS, like sub-carriers TS, which directly occupy sub-carrier data sequences, CPLS met...

  15. Restructuring of oil and gas companies in financial difficulty: the Canadian experience

    International Nuclear Information System (INIS)

    Hudec, A.J.

    1992-01-01

    The relationship, under Canadian law, between oil or gas company in financial difficulty and its various creditors is discussed. In particular, commercial bank lenders and non-defaulting co-venturers in the project are considered. The broad topics covered are: the financing of multi-party resource projects; recent developments in conventional oil and gas production loan facilities; alternative and new approaches to energy loan restructuring; minimizing the lender's environmental risk in realizing against an energy project. (UK)

  16. The protsessno-focused re-structuring of the enterprise of mechanical engineering: from the theory to practice

    OpenAIRE

    A. Afanasev

    2015-01-01

    In the developed uneasy economic conditions, re-structuring - one of the most effective methods who can influence positive change of a machine building complex, its organizational-technical, administrative and legal orientation. Necessity of a theoretical and methodical readiness of investigated problems and their practical importance have caused an urgency and a choice of a theme of article. The purpose / problems. Enterprise re-structuring as a practical embodiment of the purposes of manage...

  17. Science Lab Restructuring of a Public School Elementary and High School

    Directory of Open Access Journals (Sweden)

    Elisiane da Costa Moro

    2016-02-01

    Full Text Available This paper presents the process of restructuring the science lab of a state school in Caxias do Sul, whose main objective was to create a space where teachers could develop practical and experimental activities with their students. The restructuring of the science lab this school, was only possible through the project "More and Better Students and Teachers in Science, Mathematics, Engineering and Technologies" Initiation Program in Science and Mathematics, Engineering, Creative Technologies and Letters - PICMEL sponsored jointly by the University of Caxias do Sul, the SEDUC-RS, FAPERGS and CAPES. The project was developed at school by a teacher of physics and three high school students. Through the restructuring of the science lab, practical activities were developed and workshops where students had the opportunity to be more active in the process of teaching and learning. With the development of such activities was observed that the students were more willing to learn Science and Mathematics and could relate scientific knowledge to their daily lives, giving greater meaning to their learning.

  18. Nutritional composition in the chia seed and its processing properties on restructured ham-like products

    Directory of Open Access Journals (Sweden)

    Yi Ding

    2018-01-01

    Full Text Available Low-fat meat products always have harder texture, lower juiciness, and worse flavor. Due to their higher water-holding, water absorption, and organic molecule absorption, chia seeds (CHIA have been applied in powders, nutrition bars, breads, and cookies. Hence, the objectives of this study were to: (1 analyze the nutritional compositions in CHIA; and (2 look for the possible application of CHIA on restructured ham-like products. CHIA has high amounts of α-linolenic acid, crude polysaccharides, and also contains essential amino acids, minerals, and polyphenols. Regarding processing properties of CHIA, a combination of CHIA and carrageenan (CA increased (p<0.05 production yield of restructured ham-like products. A scanning electron microscope observation indicated that CHIA and CA addition can assist an emulsification in this ham-like product. Addition of 0.5% CA and 1.0% CHIA in this ham-like product showed the similar overall acceptance as products with added fat. Following storage at 4°C, higher (p<0.05 purge and centrifugation losses, as well as hardness of this ham-like product can be improved by adding CHIA and CA. CHIA addition also resulted in lower (p<0.05 lipid and protein oxidation, especially a 1.0% addition. In summary, due to both nutritional addition and improvements on physicochemical and sensorial properties of restructured ham-like products, CHIA seeds have great potential on the development of healthy and good-quality meat products.

  19. Current status and analysis of renewable promotional policies in Indian restructured power sector - A review

    International Nuclear Information System (INIS)

    Singh, Randhir; Sood, Yog Raj

    2011-01-01

    Restructuring has changed the traditional mission and mandates of power utilities in complex ways, and had large impacts on environmental, social, and political conditions for any particular country. At the same time, new regulatory approaches are being found for reducing environmental impacts in restructured power sectors. India has a vast supply of renewable energy resources, and it has one of the largest programs in the world for deploying renewable energy based products and systems. So this paper attempts to review the various policies and measures undertaken by Indian government for promotion of renewable energy. The aim of this paper is also to review the current policy mechanisms, especially investment- or generation-based price-driven and capacity-driven mechanisms, ranging from investment incentives for the development of renewable energy projects, feed-in tariffs, production tax incentives, tradable green certificates, and their effects upon the prospects of encouraging as well as expanding the development of renewable energy in Indian restructured power sector. This will make renewable more attractive in the Indian future electricity market. (author)

  20. Economies of scale and scope in the Danish hospital sector prior to radical restructuring plans.

    Science.gov (United States)

    Kristensen, Troels; Olsen, Kim Rose; Kilsmark, Jannie; Lauridsen, Jørgen T; Pedersen, Kjeld Møller

    2012-07-01

    The Danish hospital sector faces a significant rebuilding program driven by recent regional reform and guidelines for acute admission hospitals. Within the next 5-10 years, the number of public hospitals offering acute admission will be reduced from 35 to approximately 20 larger hospitals. As the administrative data may be biased during the middle of a restructuring process our objective was to analyze whether the configuration of Danish public hospitals was subject to economies of scale and scope prior to the restructuring plans. We estimated a quadratic cost function using panel data on the total costs for somatic treatment, casemix adjusted DRG-production values, and other cost drivers for the three years before the 2007 reforms. A short-run cost function was used to derive estimates of a long-run cost function by applying the envelope condition. Next, we estimated economies of scale and scope. We identified moderate-to-significant economies of scale and scope. This indicates that the Danish hospital sector was characterized by unexploited gains from consolidation. Our results suggest that the proposed plans have the potential to result in hospitals that are more efficient. However, post-restructuring studies elsewhere show that the strategy of horizontal integration has failed. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  1. A restructuring of the MELCOR fission product packages for the MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S.H.; Kim, K.R.; Kim, D.H.

    2004-01-01

    The RN1/RN2 packages, which are the fission product-related packages in MELCOR, have been restructured for the MIDAS computer code. MIDAS is being developed as an integrated severe accident analysis code with a user-friendly graphical user interface and a modernized data structure. To do this, the data transferring methods of the current MELCOR code are modified and adopted into the RN1/RN2 package. The data structure of the current MELCOR code using FORTRAN77 has a difficulty in grasping the meaning of the variables as well as waste of memory. New features of FORTRAN90 make it possible to allocate the storage dynamically and to user-defined data type, which leads to an efficient memory treatment and an easy understanding of the code. Restructuring of the RN1/RN2 package addressed in this paper includes a module development, subroutine modification, and the treatment of MELGEN, which generates the data file, as well as MELCOR, which is processing the calculation. The verification has been done by comparing the results of the modified code with those of the existing code. As the trends are similar to each other, it implies that the same approach could be extended to the entire code package. It is expected that the code restructuring will accelerate the code domestication thanks to a direct understanding of each variable and an easy implementation of the modified or newly developed models. (author)

  2. Effect of comminution method and raw binder system in restructured beef.

    Science.gov (United States)

    Boles, J A; Shand, P J

    1998-07-01

    The effect of particle size, method of particle size reduction (grinding, flaking, slicing) and alginate or Fibrimex(™) binding systems on processing properties of restructured beef were investigated. The size of semimembranosus muscles was reduced with a meat slicer, grinder or flaker (Comitrol) through machine openings that were 2, 4 and 8 mm in size. Bind of meat pieces in raw and cooked beef steakettes were measured. Sensory evaluation to determine the effect of method of size reduction on texture of beef steakettes was done only on steakettes manufactured with alginate. Beef steakettes manufactured with the alginate binding system had significantly (p meat for the manufacture of restructured beef steakettes resulted in a higher raw bind than did either grinding or flaking the meat. Significant (p meat was sliced than when it was ground or flaked. However, when Fibrimex was used, bind was stronger when sliced meat was used. The alginate system resulted in higher binds for cooked product when particle size was smaller but with the Fibrimex system bind was higher when particle sizes were larger. A consumer panel on alginate restructured steakettes showed no preference for the texture produced by the different methods of particle size reduction.

  3. Flexible Transmission Network Planning Considering the Impacts of Distributed Generation

    OpenAIRE

    Junhua Zhao; John Foster

    2010-01-01

    The restructuring of global power industries has introduced a number of challenges, such as conflicting planning objectives and increasing uncertainties,to transmission network planners. During the recent past, a number of distributed generation technologies also reached a stage allowing large scale implementation, which will profoundly influence the power industry, as well as the practice of transmission network expansion. In the new market environment, new approaches are needed to meet the ...

  4. Restructuring and privatisation of the elctricity economy in England and Wales. Restructuration et privatisation de l'economie electrique en Angleterre et dans le Pays de Galles

    Energy Technology Data Exchange (ETDEWEB)

    Jefferies, D. (National Grid Co. plc., Coventry (United Kingdom))

    1994-02-04

    The new structure of the electricity economy in England and Wales should lead to cost reductions due to competition at the generation level and new possibilities on the demand side. Whether price reductions can be achieved with the same quality of supply will only become apparent after a few years. The effects of privatisation and restructuring cannot yet be finally estimated. In the short term, the system is closely regulated, as the existing capacity was erected in different conditions. Only in the long term, when the system has adapted to the changed market conditions, will the real consequences and developments be shown. In the long term, the aspect of security of supply will gain in importance. (orig.)

  5. Lifetime Optimization of a Multiple Sink Wireless Sensor Network through Energy Balancing

    Directory of Open Access Journals (Sweden)

    Tapan Kumar Jain

    2015-01-01

    Full Text Available The wireless sensor network consists of small limited energy sensors which are connected to one or more sinks. The maximum energy consumption takes place in communicating the data from the nodes to the sink. Multiple sink WSN has an edge over the single sink WSN where very less energy is utilized in sending the data to the sink, as the number of hops is reduced. If the energy consumed by a node is balanced between the other nodes, the lifetime of the network is considerably increased. The network lifetime optimization is achieved by restructuring the network by modifying the neighbor nodes of a sink. Only those nodes are connected to a sink which makes the total energy of the sink less than the threshold. This energy balancing through network restructuring optimizes the network lifetime. This paper depicts this fact through simulations done in MATLAB.

  6. Mechanism of action of benzoic acid on Zygosaccharomyces bailii: effects on glycolytic metabolite levels, energy production, and intracellular pH.

    Science.gov (United States)

    Warth, A D

    1991-01-01

    The effects of benzoic acid in the preservative-resistant yeast Zygosaccharomyces bailii were studied. At concentrations of benzoic acid up to 4 mM, fermentation was stimulated and only low levels of benzoate were accumulated. Near the MIC (10 mM), fermentation was inhibited, ATP levels declined, and benzoate was accumulated to relatively higher levels. Intracellular pH was reduced but not greatly. Changes in the levels of metabolites at different external benzoic acid levels showed that glycolysis was limited at pyruvate kinase and glyceraldehyde dehydrogenase-phosphoglycerate kinase steps. Inhibition of phosphofructokinase and several other glycolytic enzymes was not responsible for the inhibition of fermentation. Instead, the results suggest that the primary action of benzoic acid in Z. bailii is to cause a general energy loss, i.e., ATP depletion. PMID:1785916

  7. Targeting molecular networks for drug research

    Directory of Open Access Journals (Sweden)

    José Pedro Pinto

    2014-06-01

    Full Text Available The study of molecular networks has recently moved into the limelight of biomedical research. While it has certainly provided us with plenty of new insights into cellular mechanisms, the challenge now is how to modify or even restructure these networks. This is especially true for human diseases, which can be regarded as manifestations of distorted states of molecular networks. Of the possible interventions for altering networks, the use of drugs is presently the most feasible. In this mini-review, we present and discuss some exemplary approaches of how analysis of molecular interaction networks can contribute to pharmacology (e.g., by identifying new drug targets or prediction of drug side effects, as well as listing pointers to relevant resources and software to guide future research. We also outline recent progress in the use of drugs for in vitro reprogramming of cells, which constitutes an example par excellence for altering molecular interaction networks with drugs.

  8. Differential expression of genes involved in alternative glycolytic pathways, phosphorus scavenging and recycling in response to aluminum and phosphorus interactions in Citrus roots.

    Science.gov (United States)

    Yang, Lin-Tong; Jiang, Huan-Xin; Qi, Yi-Ping; Chen, Li-Song

    2012-05-01

    The objective was to determine the possible links between the expression levels of genes involved in alternative glycolytic pathways, phosphorus (P) scavenging and recycling and Citrus tolerance to aluminum (Al) and/or P-deficiency. 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2 mM AlCl(3)·6H(2)O × 0, 50 and 200 μM KH(2)PO(4). C. sinensis displayed more tolerant to Al and P-deficiency than C. grandis. Under Al stress, C. sinensis accumulated more Al in roots and less Al in shoots than C. grandis. P concentration was higher in C. sinensis shoots and roots than in C. grandis ones. C. sinensis roots secreted more malate and citrate than C. grandis ones when exposed to Al. Al-induced-secretion of malate and citrate by excised roots from Al-treated seedlings decreased with increasing P supply. Al-induced-secretion of malate and citrate from roots and Al precipitation by P in roots might be responsible for Al-tolerance of C. sinensis. qRT-PCR analysis showed that Al-activated malate transporter (ALMT1), ATP-dependent phosphofructokinase (ATP-PFK), pyrophosphate-dependent phosphofructokinase (PPi-PFK), tonoplast adenosine-triphosphatase subunit A (V-ATPase A), tonoplast pyrophosphatase (V-PPiase), pyruvate kinase (PK), acid phosphatase (APase), phosphoenolpyruvate carboxylase (PEPC), malic enzyme (ME) and malate dehydrogenase (MDH) genes might contribute to the tolerance of Citrus to Al and/or P-deficiency, but any single gene could not explain the differences between the two species. Citrus tolerance to Al and/or P-deficiency might be caused by the coordinated regulation of gene expression involved in alternative glycolytic pathways, P scavenging and recycling.

  9. European ecological networks and greenways

    DEFF Research Database (Denmark)

    Kristiansen, Ib; Jongman, Rob H.G.; Kulvik, Mart

    2004-01-01

    In the context of European integration, networks are becoming increasingly important in both social and ecological sense. Since the beginning of the 1990s, societal and scientific exchanges are being restructured as the conceptual approaches towards new nature conservation strategies have been...... renewed. Within the framework of nature conservation, the notion of an ecological network has become increasingly important. Throughout Europe, regional and national approaches are in different phases of development, which are all based on recent landscape ecological principles. Ecological networks...... are interpreted in a variety of ways depending on different historical roots of nature conservation, planning and scientific traditions, different geographical and administrative levels, different land uses, and in the end the political decision-making is dependent on actors with different land use interests...

  10. Introducing renewable energy and industrial restructuring to reduce GHG emission: Application of a dynamic simulation model

    International Nuclear Information System (INIS)

    Song, Junnian; Yang, Wei; Higano, Yoshiro; Wang, Xian’en

    2015-01-01

    Highlights: • Renewable energy development is expanded and introduced into socioeconomic activities. • A dynamic optimization simulation model is developed based on input–output approach. • Regional economic, energy and environmental impacts are assessed dynamically. • Industrial and energy structure is adjusted optimally for GHG emission reduction. - Abstract: Specifying the renewable energy development as new energy industries to be newly introduced into current socioeconomic activities, this study develops a dynamic simulation model with input–output approach to make comprehensive assessment of the impacts on economic development, energy consumption and GHG emission under distinct levels of GHG emission constraints involving targeted GHG emission reduction policies (ERPs) and industrial restructuring. The model is applied to Jilin City to conduct 16 terms of dynamic simulation work with GRP as objective function subject to mass, value and energy balances aided by the extended input–output table with renewable energy industries introduced. Simulation results indicate that achievement of GHG emission reduction target is contributed by renewable energy industries, ERPs and industrial restructuring collectively, which reshape the terminal energy consumption structure with a larger proportion of renewable energy. Wind power, hydropower and biomass combustion power industries account for more in the power generation structure implying better industrial prospects. Mining, chemical, petroleum processing, non-metal, metal and thermal power industries are major targets for industrial restructuring. This method is crucial for understanding the role of renewable energy development in GHG mitigation efforts and other energy-related planning settings, allowing to explore the optimal level for relationships among all socioeconomic activities and facilitate to simultaneous pursuit of economic development, energy utilization and environmental preservation

  11. An evaluation of the proposed organisation restructuring at Kadoma city, 2015.

    Science.gov (United States)

    Muringazuva, A Caroline; Chirundu, Daniel; Shamu, Shepherd; Shambira, Gerald; Gombe, Notion; Juru, Tsitsi; Bangure, Donewell; Tshimanga, Mufuta

    2017-01-01

    Restructuring is the corporate management term for the act of reorganizing the legal, operational, or other structures of a company for the purpose of making it more profitable or better organized for its present needs. However, preparing an organization to accept and welcome any change is crucial. There is concern though over poor service delivery, untimely payment of workers, top management structure which is thought to be top heavy and employee costs taking (58%) of total expenditure. A descriptive cross sectional study was carried out. A cost benefit analysis was used to assess the cost and benefits of the proposed retrenchment exercise. A descriptive cross sectional study survey was conducted to assess the workers' perceptions towards the proposed restructuring exercise. A pretested self-administered questionnaire was used for data collection and data were analysed using EpiInfoTM (CDC 2012).Written informed consent was obtained from all study participants. Sixty nine percent of the respondents were males. The median years working for the organisation was 8 years (Q1=1; Q3=17). The total income was surpassed by expenditure with USD$11 000 and 52% of expenditures was going towards employment costs. A midyear financial review showed that 1% was channeled towards capital expenditure 2% on repairs and maintenance and employee costs accounting to 58% of all incurred expenditure. Current departmental salary budget amounted to USD 3,3million dollars. Estimated salary costs for the proposed departmental structures amount to USD 3,8 million dollars. Comparison of the current and proposed structure showed that the proposed structure costs USD$486 000 more. Projected benefits of the proposed structure aims to improve service delivery from 60%-85% . Unlike managers, lower levels workers did not want the exercise to be carried out. The proposed structure has higher costs than the current structure but with more benefits in terms of service delivery. Generally workers

  12. Citizen and consumer attitudes towards electricity industry restructuring : an Ontario (Canada) case-study

    International Nuclear Information System (INIS)

    Rowlands, I.; Parker, P.; Scott, D.

    2001-01-01

    This report examines public attitudes regarding electricity restructuring in Ontario. A survey from the Waterloo region in western Ontario provided responses to questions about attitudes towards restructuring and strategies for advancing environmental goals. A 158-item survey was sent to 1,110 individuals, between September 2000 and April 2001. A total of 474 questionnaires were answered, for a response rate of 43 per cent. It was noted that although the response rate was high, the survey may not be representative because the respondents were older, better educated, and wealthier than the general population. In addition, the respondents had previously indicated they were willing to pay $25 for a home energy evaluation, suggesting a special interest in issues regarding energy and the environment. Respondents were first asked whether they agreed, disagreed or were not sure about the need to restructure, whether a single government power company should supply electricity, or whether they were pleased to choose a power company of their choice. The respondents were then asked to rank the importance of the price of electricity, the quality of customer service, whether the electricity is generated in southern Ontario, the environmental effects of the electricity produced by the company, the reputation of the company, and reliability of electricity. Price and reliability ranked as the highest priorities by respondents. Citizens' attitudes toward premium-priced green electricity were also examined. The general conclusion was that while many respondents wanted a sustainable electricity system, most of them will look for the cheapest electricity product once the market is opened. 6 refs., 3 tabs

  13. 1992 Mary J. Nielubowicz Award recipient. Nursing organizational restructure: choosing to change.

    Science.gov (United States)

    Ahrens, W D

    1993-04-01

    Major organizational change frequently results in fear, anxiety, and resistance. Change is often initiated in response to a perceived threat. However, intentional changes to historical patterns of nursing care delivery are now more prevalent, in the absence of crisis, as leaders anticipate new challenges and fulfill today's nurses' need for high-level professional satisfaction. This essay describes factors which inspired nursing organizational restructure at a naval hospital. The specific modifications made and strategies for managing them are reviewed in the context of using planned change principles and risk-taking behavior to achieve organizational growth.

  14. A Primer on Electric Utilities, Deregulation, and Restructuring of U.S. Electricity Markets

    Energy Technology Data Exchange (ETDEWEB)

    Warwick, William M.

    2002-06-03

    This primer is offered as an introduction to utility restructuring to better prepare readers for ongoing changes in public utilities and associated energy markets. It is written for use by individuals with responsibility for the management of facilities that use energy, including energy managers, procurement staff, and managers with responsibility for facility operations and budgets. The primer was prepared by the Pacific Northwest National Laboratory under sponsorship from the U.S. Department of Energy?s Federal Energy Management Program. The impetus for this primer originally came from the Government Services Administration who supported its initial development.

  15. Direct Load Control (DLC) Considering Nodal Interrupted Energy Assessment Rate (NIEAR) in Restructured Power Systems

    DEFF Research Database (Denmark)

    Wu, Qiuwei; Wang, Peng; Goel, Lalit

    2010-01-01

    is used as the bids from the ACL customers, is utilized to determine the direct monetary compensation to the ACL customers. The proposed scheme was investigated for the PoolCo electricity market. The optimal DLC scheme is determined based on the minimum system operating cost which is comprised......A direct load control (DLC) scheme of air conditioning loads (ACL) considering direct monetary compensation to ACL customers for the service interruption caused by the DLC program is proposed in this paper for restructured power systems. The nodal interrupted energy assessment rate (NIEAR), which...

  16. Value Chain Restructuring, Work Organization and Labour Outcomes in Football Manufacturing in India

    DEFF Research Database (Denmark)

    Khara, Navjote; Lund-Thomsen, Peter

    2012-01-01

    this aim, the article develops a new analytical framework based upon the global value chain approach. Applying this framework to the football manufacturing industry of Jalandhar, India, the article concludes that value chain restructuring produces both new forms of work organization and highly gendered......This article seeks to understand why developing country suppliers internalize or outsource labour-intensive aspects of production as well as how these internalization/externalization processes affect working conditions in export-orientated industries in the global South. In order to achieve...

  17. The restructuring and internalization of retail trade in Poland between 1990 and 2007

    Directory of Open Access Journals (Sweden)

    Michał Gazdecki

    2009-01-01

    Full Text Available The restructuring and internationalisation processes of retail trade in Poland were presented in the article. Two main aspects were taken into consideration: changes of shops number and entrance as well as development of foreign enterprises in the Polish market. Most transformations of domestic retail trade were initiated by foreign enterprises starting business in Poland. The pace of changes in the domestic retail trade visibly slowed down after 2002. In the successive years changes were continued, but they were slower and their character also changed.

  18. Restructuring, Rationalizing and Modernizing Higher Education Sector in the Republic of Moldova

    DEFF Research Database (Denmark)

    This book presents the recommendations on restructuring, rationalization and modernization of higher education sector in Moldova. It consists of three parts. In part one, legislative proposals are presented in which inter alia the new structure of the sector is suggested, new key players......, Romania, Scotland and Sweden and discusses best practices internal to a university on organizational, financial, human resource and academic autonomy. This book is the final, third major deliverable of the EUniAM project. It builds on and is derived from the first two deliverables of the project: „The...

  19. NEW BUSINESS MODELS, THE RESTRUCTURE OF COMPETITION AND IMPLICATIONS ON BUSINESS MANAGEMENT AND TRADE STATISTICS

    Directory of Open Access Journals (Sweden)

    SERGHEI MARGULESCU

    2014-11-01

    Full Text Available Economists and managers should be aware today of the fact that some concepts and economic mechanisms need to be reconsidered in the context of the new aspects of globalization. The appearance of new types of business and new trade patterns on the international stage has led to the restructure of worldwide competition. “Traditional” competition between companies is replaced, in the context of the “globally integrated businesses” and the new division of labour based on the “functional” specialization, by new types of competition. This has a few implications on business management and on foreign trade statistics and analysis as well.

  20. The Molecular Restructuring of Classical Desserts by Using Food Industry By-Products

    Directory of Open Access Journals (Sweden)

    Gabriela PRECUP

    2017-11-01

    The present work focused on achieving a new type of dessert, a “Sweetburger”, by restructuring its molecular components with the aid of molecular gastronomy techniques and using agro-industrial wastes (beetroot and sour cherry pulp and peels, melon pomace. The innovative dessert has been obtained and the bioactive compounds contained in the agro-industrial wastes have been characterized using the HPLC method, Folin-Ciocalteu and spectrophotometry. The total polyphenol content from sour cherries waste was significant, 508,9 mg gallic acid/100 g. The total anthocyanin content from beetroot pulp was 0,117 mg/ 100 g.

  1. Electricity industry restructuring and distribution Grids' Take-Over. A survey of foreign case studies

    International Nuclear Information System (INIS)

    Indigenti, S.

    1999-01-01

    The paper surveys some case studies drawn from five foreign countries experience in distribution restructuring and property changes. From transaction prices has been derived the indirect distribution grid evaluation by the investors. The resulting values are widespread and cannot be taken as a sound reference for similar future property transfers in Italy. This paper may be looked at as a methodological guides that will likely shed some light on the possible implementations of the recent electricity bill 79/99 named Decreto Bersani [it

  2. Surface restructuring of lignite by bio-char of Cuminum cyminum - Exploring the prospects in defluoridation followed by fuel applications

    Science.gov (United States)

    Msagati, T. A. M.; Mamba, B. B.; Sivasankar, V.; Omine, Kiyoshi

    2014-05-01

    Recently, there has been an interest in the areas of developing new carbon materials for fluoride removal applications. The development of new carbon materials is of recent choice which involves the synthesis of hybrid carbon from various sources. In this context, the present contribution is made to focus on the study the restructured surface of lignite using a bio-material called Cuminum cyminum. The restructured lignite (RSL) was synthesized with an improved carbon content of 13% and its BET surface area was found to be 3.12 times greater than lignite (L). The amorphous nature of lignite and RSL was quite explicable from XRD studies. SEM studies exhibited a fibrous and finer surface of lignite which was well restructured into a semi-melt (5 μm) surface for RSL. Defluoridation potential of Restructured Lignite (15.8 mg g-1) was greater than the lignite (13.8 mg g-1) at pH 7.93 ± 0.03. Kinetic and isotherm parameters derived from various models helped in comprehending the nature and dynamics of fluoride sorption. Both the normal and the restructured lignite were found to be consistent with its fluoride uptake of 57% and 60% respectively even after fifth cycle of regeneration. High heating values of 22.01 MJ kg-1 and 26.90 MJ kg-1 respectively for lignite and RSL deemed their additional application as fuel materials.

  3. How do Restructuring Processes Influence Low- and Unskilled Immigrant and Nonimmigrant Workers and their Managers in a Norwegian Hospital?

    Directory of Open Access Journals (Sweden)

    Heidi Enehaug

    2015-01-01

    Full Text Available It is a well-known fact that workplace restructuring has undesirable effects on the psychosocial work environment, health, and sick leave, but no attention has been given to the health effects of work environments characterized by restructuring, a multicultural staff, and a strong socioeconomic occupational hierarchy. In this casestudy, we examine a large Norwegian hospital in which all of these features are present. Through in-depth interviews with employees and their managers we investigate the healthiness of the restructuring process, and the consequences of the restructuring process on the work environment, subjective health, and sick leave. Results show that immigrant workers received less information, had higher level of frustration and less control over work, and experienced a decrease in well-being, autonomy, and social support. Immigrant worker vulnerability, that is, the handicap of poor understanding of the Norwegian language and a lack of understanding of general and local organizational norms and practices in the Norwegian workplace, is an important explanation. Immigrant workers with a poor Norwegian language understanding are even worse off. We conclude that a strong occupational hierarchy within the hospital exerts an overall influence on the position of low/unskilled employees in the restructuring process as well as their perception of and involvement in it.

  4. Power industry restructuring and eco-efficiency changes: A new slacks-based model in Malmquist–Luenberger Index measurement

    International Nuclear Information System (INIS)

    Arabi, Behrouz; Munisamy, Susila; Emrouznejad, Ali; Shadman, Foroogh

    2014-01-01

    Measuring variations in efficiency and its extension, eco-efficiency, during a restructuring period in different industries has always been a point of interest for regulators and policy makers. This paper assesses the impacts of restructuring of procurement in the Iranian power industry on the performance of power plants. We introduce a new slacks-based model for Malmquist–Luenberger (ML) Index measurement and apply it to the power plants to calculate the efficiency, eco-efficiency, and technological changes over the 8-year period (2003–2010) of restructuring in the power industry. The results reveal that although the restructuring had different effects on the individual power plants, the overall growth in the eco-efficiency of the sector was mainly due to advances in pure technology. We also assess the correlation between efficiency and eco-efficiency of the power plants, which indicates a close relationship between these two steps, thus lending support to the incorporation of environmental factors in efficiency analysis. - Highlights: • We introduce a new slack-based model incorporating bad outputs to measure eco-efficiency. • Eco-efficiency change of power plants is measured over a restructuring period. • A success to enhance the eco-efficiency is revealed. • A close relationship between efficiency and eco-efficiency is shown

  5. Restructured low-fat cooked ham containing liquid whey fortified with lactulose.

    Science.gov (United States)

    Oliveira, Cristiane A; Massingue, Armando A; Moura, Ana Paula R; Fontes, Paulo Rogério; Ramos, Alcinéia Ls; Ramos, Eduardo M

    2018-01-01

    Current health concerns have driven consumers to request products with nutritional and physiological advantages, which can be achieved by using prebiotic ingredients. Lactulose is a prebiotic with excellent functional properties and can be easily incorporated into meat products through the addition of liquid whey. This study investigated the technological and sensorial quality of restructured cooked ham elaborated without liquid whey added (control) and with liquid whey containing different contents (0, 30, 60 and 100 g kg -1 ) of lactulose. Liquid whey did not change any technological or sensorial characteristics of the product, but the general acceptability decreased due to addition of lactulose. Samples with higher lactulose concentrations had lower moisture content, pH and refreezing loss and increased carbohydrate content. Control and whey added samples had higher lightness and lower intense color than samples with lactulose. Liquid whey additions with higher lactulose content increased hardness and chewiness of the samples. Restructured cooked hams formulated with liquid whey and 30 g kg -1 of lactulose had minimal effects on the technological properties and sensory characteristics and, due to the possible benefits conferred by the prebiotic, is a potential alternative to provide meat products with prebiotic activity. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  6. Expectations for renewable energy under market restructuring: the U.S. experience

    International Nuclear Information System (INIS)

    Heiman, M.K.

    2006-01-01

    The 1992 Energy Policy Act encouraged states to open up electricity provision to market competition. Many analysts predicted that renewable energy would take off in the deregulated market where consumers could choose their power provider and utilities would no longer be enticed to build large central power plants under guaranteed rates of return. This article outlines the flaws with that expectation. Absent a strong federal commitment, the states continue to lead with support for renewable energy. However the base from which to expand is so low, and the level of support so tenuous, that renewable energy will continue to play only a minor role in meeting U.S. energy demands with current policy in place. Furthermore, the evidence does not support the expectation that market restructuring, in and of itself, leads to a stronger state commitment to renewable energy. The failure of renewable energy to become a major component of the U.S. energy mix is not due to any intrinsic problem with the technology employed, nor with the cost of generation. Rather weak penetration may be attributed to broader forces exacerbated by market restructuring and overcome only through strong and reliable federal intervention in support of renewable energy. (author)

  7. The liberalisation of the European electricity market : an unstructured restructuring process?

    International Nuclear Information System (INIS)

    Boisseleau, F.; Hakvoort, R.

    2005-01-01

    The European Union (EU) directive 96/92/EC defines common rules for the generation, transmission and distribution of electricity, paving the way for the liberalization of the electricity markets of EU member states. Member states are obliged to open their national electricity supply markets, meaning that eligible customers can choose their own suppliers. This paper discussed the process by which the countries of the EU have restructured their electricity markets, arguing that the process has focused on legal and organizational issues, rather than specific prescriptions for the economic design of the market. Although the objective of the EU directive was to create a competitive market, restructuring has led to 15 or more fragmented markets, each liberalized to a different degree and shaped following a wide range of different principles. Areas where the EU has failed to provide a coherent market view were discussed. Issues concerning short-term market arrangements, congestion management and long-term investment were examined. Section 1 of the paper focused on policy issues in the liberalization process, while section 2 emphasized the importance of market design. Section 3 addressed the need for market monitoring and the issue of market power. It was concluded that liberalization is only one step in the process that alone cannot deliver the expected benefits of single integrated European-wide market. At present, the European electricity market is hindered by a lack of proper design and sufficient transparency. 32 refs., 3 tabs., 4 figs

  8. Restructuring of burnup sensitivity analysis code system by using an object-oriented design approach

    International Nuclear Information System (INIS)

    Kenji, Yokoyama; Makoto, Ishikawa; Masahiro, Tatsumi; Hideaki, Hyoudou

    2005-01-01

    A new burnup sensitivity analysis code system was developed with help from the object-oriented technique and written in Python language. It was confirmed that they are powerful to support complex numerical calculation procedure such as reactor burnup sensitivity analysis. The new burnup sensitivity analysis code system PSAGEP was restructured from a complicated old code system and reborn as a user-friendly code system which can calculate the sensitivity coefficients of the nuclear characteristics considering multicycle burnup effect based on the generalized perturbation theory (GPT). A new encapsulation framework for conventional codes written in Fortran was developed. This framework supported to restructure the software architecture of the old code system by hiding implementation details and allowed users of the new code system to easily calculate the burnup sensitivity coefficients. The framework can be applied to the other development projects since it is carefully designed to be independent from PSAGEP. Numerical results of the burnup sensitivity coefficient of a typical fast breeder reactor were given with components based on GPT and the multicycle burnup effects on the sensitivity coefficient were discussed. (authors)

  9. Interception of values as a result of the business model restructuring – case study

    Directory of Open Access Journals (Sweden)

    Nogalski Bogdan

    2017-12-01

    Full Text Available The main objective of this paper is to present a case of value interception as a result of restructuring the business model of a manufacturing company that operates in the agricultural machinery sector. A company that focuses on core activities in the value chain and commissions the manufacturing of most components to specialised suppliers – as a result of restructuring – becomes an integrator that controls all parts of the supply chain; from obtaining a raw material, through its own production of a possibly large number of components, to the distribution of a finished composite product. The framework of the conducted research featured the identification of the relationships occurring between own production of components comprising a given product, and an alternative solution, i.e. possibility of acquiring them by way of co-operation. The authors assumed that a derivative of the value intercepted in the finished product implementation process is the number of components manufactured using own production resources.

  10. Organizational restructuring and reconstruction of identity: a case study in the company of telecommunications

    Directory of Open Access Journals (Sweden)

    Daniela Cristina Soares

    2010-01-01

    Full Text Available The main objective of this research is to analyze the impacts that re-structuring the telephone operators at the cellular operators has on the identity of the workers. The theme talks about the profound transformations which occur in the world of work, derived from the link between globalization and the economy, the ongoing advancements in technology and the new immerging forms of Company management. The methodology adopted is of qualitative nature whose data was collected by means of semi-structured interviews. Theoretically speaking, this research boarders two fundamental themes; the current changes that occur in relationships at the workplace and the effects these changes have on the identity of professionals. The survey results show that the process of organizational restructuring culminated with the simultaneous resignation of 382 officials from the area of call center and was conducted in a cruel and merciless. The managers were informed of the decision by the company two days before the action occurredand the area of human resources was limited to bureaucratic aspects of care. The experience generated by the interviewees feelings of surprise, disappointment, sadness and anguish, affecting negatively the process of construction and reconstruction of identities.

  11. Electric power industry restructuring in Australia: Lessons from down-under. Occasional paper No. 20

    Energy Technology Data Exchange (ETDEWEB)

    Ray, D. [Univ. of Wisconsin, Madison, WI (United States)

    1997-01-01

    Australia`s electric power industry (EPI) is undergoing major restructuring. This restructuring includes commercialization of state-owned electric organization through privatization and through corporatization into separate governmental business units; structural unbundling of generation, transmission, retailing, and distribution; and creation of a National Electricity Market (NEM) organized as a centralized, market-based trading pool for buying and selling electricity. The principal rationales for change in the EPI were the related needs of enhancing international competitiveness, improving productivity, and lowering electric rates. Reducing public debt through privatization also played an important role. Reforms in the EPI are part of the overall economic reform package that is being implemented in Australia. Enhancing efficiency in the economy through competition is a key objective of the reforms. As the need for reform was being discussed in the early 1990s, Australia`s previous prime minister, Paul Keating, observed that {open_quotes}the engine which drives efficiency is free and open competition.{close_quotes} The optimism about the economic benefits of the full package of reforms across the different sectors of the economy, including the electricity industry, is reflected in estimated benefits of a 5.5 percent annual increase in real gross domestic product and the creation of 30,000 more jobs. The largest source of the benefits (estimated at 25 percent of total benefits) was projected to come from reform of the electricity and gas sectors.

  12. Increased injury rates after the restructure of Germany's national second league of team handball.

    Science.gov (United States)

    Luig, Patrick; Krutsch, Werner; Nerlich, Michael; Henke, Thomas; Klein, Christian; Bloch, Hendrik; Platen, Petra; Achenbach, Leonard

    2018-02-05

    Scientific injury data in men's professional team handball injuries are rare and even less scientific information exists on injury prevention. In 2011, Germany's national second team handball league was restructured by merging the existing two regional leagues into one league. This study evaluates the injury patterns in professional team handball and compares the injury rates between the first and second league before and after the restructure. All players of Germany's national first and second men's team handball leagues have mandatory trauma insurance with the same insurance company. This retrospective cohort study analysed the injury data of three consecutive seasons 2010-2013 using standardized injury definitions. 1194 professional team handball players were included in this study. The majority of severe injuries affected the lower extremities, shoulders, and hands. The average injury incidence significantly differed between the first (4.9 injuries per 1000 h) and the second league (3.9 per 1000 h, p team handball league and presents details on prevalence, incidence, and patterns of injury in professional men's team handball. This study is an important basis for developing injury prevention strategies that should focus on the shoulders, hands, and lower extremities and on reducing the number of matches and travel burden. III.

  13. Privatization and re-structuration processes of electric monopolies: the case of developing countries

    International Nuclear Information System (INIS)

    Smida, S.

    2002-11-01

    In the new context of financial sources contraction from the eighties, the developing countries had to accept and engage a series of reform. Besides the well advanced re-structuration of the industries known as competitive, the public utilities have not been ignored. Privatization and the development of private participation in electric industries are, since the beginning of the nineties, in the centre of this evolution. In order to understand this change, we started with the following question. Is public ownership really inefficient? In other words, is privatization an actual alternative as it is implied by some economic development? Our aim in this thesis is to verify empirically if the ownership and/or private management in electric monopolies are preferable to the public form. The first part of this work deals with the theoretical and conceptual aspects. It primarily deals with operation, regulation and by and large with the traditional electric industries organisation. There is also a quotation of the failure of the first reforms as the contracts of performance ('contrats de plan'). The second part is essentially factual. It deals with the analysis and valuation of electric privatization and re-structuration in some developing countries. It appears from this study, that if privatization remains 'impossible' to avoid, it's widely because of public organisation and management failure. Nevertheless, the idea that private form is higher and more efficient than the public form is not necessarily justified. Indeed, other considerations seem to be more important as the type of regulation and the competitive intensity. (author)

  14. An Alderfer perspective of the higher education restructuring in South Africa

    Directory of Open Access Journals (Sweden)

    C. A. Arnolds

    2005-10-01

    Full Text Available The consequences of the restructuring of higher education in South Africa have not yet been thoroughly investigated. This study investigates the impact of the abovementioned restructuring on employee motivation (as measured by Alderfer’s ERG theory, organisational commitment and job performance. The results show that the respondents exhibit low levels of organisational commitment and low satisfaction with monetary remuneration and fringe benefits. The results, however, show high levels of satisfaction with growth factors, peer relations and performance intentions. These results are interpreted in the light of the multiple regression analyses conducted of the interrelationships among the variables. Opsomming Die nagevolge van die herkonstruksie van hër-onderwys in Suid-Afrika is nog nie deeglik ondersoek nie. Hierdie studie ondersoek die invloed wat bogenoemde rekonstruksie op die motivering (soos gemeet deur Alderfer se teorie, organisatories toegewydheid en werksprestasie van werknemers het. Die resultate toon dat die respondente lae vlakke van organisatoriese toegewydheid en lae tevredenheid ten opsigte van monetêre beloning en byvoordele tentoonstel. Die resultate toon egter hoë vlakke van tevredenheid in soverre groeifaktore, kollegiale verhoudinge en werkprestasievoornemens betref. Hierdie resultate word vertolk teen die agtergrond van veelvoudige regressie-analises wat op die inderlinge verwantskappe tussen die veranderlikes uitgevoer is.

  15. Some design lessons from market-based greenhouse gas regulation in the restructured Australian electricity industry

    International Nuclear Information System (INIS)

    MacGill, Iain; Outhred, Hugh; Nolles, Karel

    2006-01-01

    There is growing worldwide interest in the use of market-based policy instruments for climate change regulation in the electricity sector. These mechanisms would seem to offer some efficiency and flexibility advantages over more traditional regulatory approaches, while being highly compatible with competitive market-based electricity industries. Australia has been an early and enthusiastic adopter of both electricity industry restructuring and market-based environmental instruments. This paper first outlines some of these recent policy developments. In particular, we describe the objectives, design and outcomes to date of electricity industry restructuring, the Mandatory Renewable Energy Target, the NSW Greenhouse Benchmarks, the Queensland 13% Gas scheme and Government accredited Green Power. From this, we draw some key design lessons for such market-based instruments. These include the perils of abstraction in scheme design, the vital importance of setting appropriate baselines in 'baseline and credit' schemes, the possibility that such measures may interact in ways that reduce their environmental effectiveness, 'market for lemon' risks with tradable instruments that have measurement, verification or 'additionality' difficulties, and the challenges of creating transparent liquid markets for these mechanisms. The mixed performance of these Australian schemes to date illustrates the need for great care in designing such market-based approaches

  16. Restructuring and risk-reduction in mining: employment implications for northern Sweden

    Directory of Open Access Journals (Sweden)

    Örjan Pettersson

    2010-11-01

    Full Text Available In the past, employment in northern Sweden has been largely dependent on natural resources. Shifting demands and price fluctuations for raw materials have caused boom periods as well as times of crisis in local communities. During the first decade of the 21st century, increasing global demand for minerals resulted in substantial investments in the Swedish mining industry. The purpose of this article is to assess the importance of mining for employment in the county of Västerbotten, northern Sweden, by focusing on the time period after 1990. Mining employment constitutes a rather small part of all employment in the study area, due to a restructuring process that started in the 1960s. However, results show that mining employment has increased slightly, especially after 2002. Global demand for minerals and related technology and services make it reasonable to believe that this change will have a deeper significance for employment opportunities in the study area. Restructuring in mining generates new business opportunities in subcontracting, consultancy and equipment production, but also creates new challenges. Consequently, it is important to make strategic decisions on regional and local levels concerning how to make use of the development in the mining industry to stimulate long-term regional employment growth.

  17. The protsessno-focused re-structuring of the enterprise of mechanical engineering: from the theory to practice

    Directory of Open Access Journals (Sweden)

    A. Afanasev

    2015-01-01

    Full Text Available In the developed uneasy economic conditions, re-structuring - one of the most effective methods who can influence positive change of a machine building complex, its organizational-technical, administrative and legal orientation. Necessity of a theoretical and methodical readiness of investigated problems and their practical importance have caused an urgency and a choice of a theme of article. The purpose / problems. Enterprise re-structuring as a practical embodiment of the purposes of managers, is a difficult, multifactorial problem. Accordingly, the decision of this problem, namely construction of the protsessno-focused re-structuring of the enterprise demands the complex, system approach. Article purpose is – the description of methodology of one of possible approaches to transformation of re-structuring of the enterprise of mechanical engineering from the point of view of practical management.Methodology. In article the methodology is resulted, allowing to solve the questions connected with re-structuring at the enterprises of mechanical engineering owing to consistency .Results. The present article is short describes the practical scientifically-methodical approach in which frameworks was construction of the protsessno-focused re-structuring of the enterprise of mechanical engineering on an example of the largest enterprise of Privolzhsky federal district of Open Society "Кamaz" is offered.Conclusions / the importance. Undoubtedly, offered approach – is not unique. However it has essential advantages before others – the consistency and application in aggregate both the conventional tools of management, and the original workings out which have proved the practical suitability in the conditions of the Russian practice. This approach – not panacea from all troubles of the real enterprise. Nevertheless, as practice shows, he allows to reach notable successes at carrying out of transformations real, instead of the

  18. Shell's Role in the Future European Gas Scene - Is a Major Market Restructuring Under Way?

    Energy Technology Data Exchange (ETDEWEB)

    Segundo, Karen de

    1999-07-01

    The European gas market is being restructured by a combination of major global forces. Environmental considerations like the Kyoto Agreement favour the use of gas. But in the medium-term, supplies may be limited by low energy prices. The political drive for increased competition has manifested itself as the EU Gas Directive, which came into force in 1998. Shell believes that the European industry must progressively adapt to this restructuring. Flexible mechanisms like tradable emission permits should be used to generate investment capital for new gas supply projects. Moreover, both industry and regulators should take a positive and constructive approach towards increasing competition in the marketplace.

  19. Comparison of a gratitude-based and cognitive restructuring intervention for body dissatisfaction and dysfunctional eating behavior in college women.

    Science.gov (United States)

    Wolfe, Wendy L; Patterson, Kaitlyn

    2017-01-01

    Researchers have investigated the efficacy of a gratitude intervention for decreasing body dissatisfaction (BD) in an internet treatment-seeking sample and demonstrated it worked equally well to decrease BD as cognitive restructuring. We extend this research by testing the efficacy of a gratitude intervention on BD, along with common sequelae of BD: dysfunctional eating, negative mood, and depressive symptoms. Females were randomly assigned to Gratitude, Cognitive Restructuring, or Control conditions. Pre- to post-intervention period comparisons found the gratitude intervention to perform better than the other conditions at increasing body esteem, decreasing BD, reducing dysfunctional eating, and reducing depressive symptoms.

  20. Fiscal 2000 report on result of research information network project for global environmental technologies; 2000 nendo chikyu kankyo taisaku gijutsu no kenkyu joho network jigyo seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    With the purpose of sharing and exchanging in real time information, basic research results, data, etc., concerning global environmental conservation technologies, the RITE's homepage on the Internet was restructured in the system in order to cope with the advance of communication technologies and the expansion of information volume. In the restructure of the information providing facilities, the equipment such as a web server was renewed, with the network function enhanced. Thesis retrieving speed was increased by the reinforcement of a thesis data base engine. Access speed was improved through the integration with the homepage publication program. Countermeasures against computer virus and crackers were reinforced. In the restructure of the homepage, with the intention of speeding up exchange of R and D related information, infrastructure was rebuilt for enabling Website operation by each laboratory. In the management of the system, Fire-Wall has been maintained and managed to stabilize the server operation and to prevent attacks against the homepage. (NEDO)

  1. Restructuring: new relationships between the oil companies and the upstream oil firms; Alliances et restructurations: nouvelles relations entre maitres d'oeuvre et parapetrolier

    Energy Technology Data Exchange (ETDEWEB)

    Barreau, S

    2001-11-01

    Since the 1986 oil shock, international oil companies have focused on their base competencies, concentrating on activities viewed as their core businesses and steadily increasing the number of tasks to be subcontracted to the upstream oil and gas service sector. The upstream oil and gas service companies had to be restructured to face this new challenge. The strategies they launched at the end of the 80's were varied. Some firms became largely integrated (Schlumberger, Baker Hughes, Halliburton) whereas other firms chose to broaden their range of services. However generally, they opted for external investment which led to an important wave of mergers and acquisitions. The first part characterizes the upstream oil and gas sector by introducing the main oil and gas service firms and their recent strategic evolution. This concludes with both an economic valuation and a typology of attempted growth strategies. To illustrate this, a matrix has been created to characterise the dynamic paths of the oil and gas service firms. The purpose of the second part is to consider the economic theories related to industrial strategies. The strategies of innovation, market protection, vertical integration and diversification have been studied to illustrate the main conclusion which is that the aim of all these strategies was to change the relationships between the oil companies and the upstream oil and gas service firms. (author)

  2. Altered expression profile of glycolytic enzymes during testicular ischemia reperfusion injury is associated with the p53/TIGAR pathway: effect of fructose 1,6-diphosphate

    Directory of Open Access Journals (Sweden)

    May Al-Maghrebi

    2016-07-01

    Full Text Available Background. Testicular ischemia reperfusion injury (tIRI is considered the mechanism underlying the pathology of testicular torsion and detorsion. Left untreated, tIRI can induce testis dysfunction, damage to spermatogenesis and possible infertility. In this study, we aimed to assess the activities and expression of glycolytic enzymes (GEs in the testis and their possible modulation during tIRI. The effect of fructose 1,6-diphosphate (FDP, a glycolytic intermediate, on tIRI was also investigated. Methods. Male Sprague-Dawley rats were divided into three groups: sham, unilateral tIRI, and tIRI + FDP (2 mg/kg. tIRI was induced by occlusion of the testicular artery for 1 h followed by 4 h of reperfusion. FDP was injected peritoneally 30 min prior to reperfusion. Histological and biochemical analyses were used to assess damage to spermatogenesis, activities of major GEs, and energy and oxidative stress markers. The relative mRNA expression of GEs was evaluated by real-time PCR. ELISA and immunohistochemistry were used to evaluate the expression of p53 and TP53-induced glycolysis and apoptosis regulator (TIGAR. Results. Histological analysis revealed tIRI-induced spermatogenic damage as represented by a significant decrease in the Johnsen biopsy score. In addition, tIRI reduced the activities of hexokinase 1, phosphofructokinase-1, glyceraldehyde 3-phosphate dehydrogenase, and lactate dehydrogenase C. However, mRNA expression downregulation was detected only for hexokinase 1, phosphoglycerate kinase 2, and lactate dehydrogenase C. ATP and NADPH depletion was also induced by tIRI and was accompanied by an increased Malondialdehyde concentration, reduced glutathione level, and reduced superoxide dismutase and catalase enzyme activities. The immunoexpression of p53 and TIGAR was markedly increased after tIRI. The above tIRI-induced alterations were attenuated by FDP treatment. Discussion. Our findings indicate that tIRI-induced spermatogenic damage is

  3. Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity

    International Nuclear Information System (INIS)

    Hopperton, Kathryn E.; Duncan, Robin E.; Bazinet, Richard P.; Archer, Michael C.

    2014-01-01

    Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from 14 C-labeled acetate to those supplied exogenously as 14 C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2–3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells. - Highlights: • Fatty acid synthase (FASN) is over-expressed in cancer but its function is unknown. • We compare utilization of

  4. Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity

    Energy Technology Data Exchange (ETDEWEB)

    Hopperton, Kathryn E., E-mail: kathryn.hopperton@mail.utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Duncan, Robin E., E-mail: robin.duncan@uwaterloo.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Bazinet, Richard P., E-mail: richard.bazinet@utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Archer, Michael C., E-mail: m.archer@utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Department of Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada)

    2014-01-15

    Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from {sup 14}C-labeled acetate to those supplied exogenously as {sup 14}C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2–3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells. - Highlights: • Fatty acid synthase (FASN) is over-expressed in cancer but its function is unknown. • We compare

  5. Energy Requirements of Odor Transduction in the Chemosensory Cilia of Olfactory Sensory Neurons Rely on Oxidative Phosphorylation and Glycolytic Processing of Extracellular Glucose.

    Science.gov (United States)

    Villar, Pablo S; Delgado, Ricardo; Vergara, Cecilia; Reyes, Juan G; Bacigalupo, Juan

    2017-06-07

    The mechanisms that power the physiological events occurring in cilia, flagella, and microvilli are of fundamental importance for the functions of these important and ubicuous organelles. The olfactory epithelium is mostly populated by ciliated olfactory sensory neurons (OSNs) and surrounding sustentacular cells (SCs) with apical microvilli. The only OSN dendrite extends to the surface forming a knob projecting several chemosensory cilia of ∼50 × 0.2 μm, devoid of inner membranes embedded in a mucus layer. Upon odorant binding, odor receptors couple to G-protein activating adenylyl cyclase, producing cAMP. cAMP opens cyclic nucleotide-gated channels allowing a Ca 2+ influx that opens Ca 2+ -activated Cl - channels, generating the receptor potential. Many enzymes are activated in chemotransduction to hydrolyze ATP. The knob contains approximately two mitochondria; assuming that the cilia ATP is 1 mm and diffuses along it at ∼10 μm in 500 ms, ATP from the knob mitochondria may not fulfill the demands of transduction over the full length of the cilium, which suggests an additional ATP source. We measured millimolar glucose in rat mucus; we detected glucose transporter GLUT3 in rat and toad ( Caudiverbera caudiverbera ) OSN cilia, SC microvilli, and glycolytic enzymes in rat cilia. We also found that the cilia and knob can incorporate and accumulate 2-deoxyglucose (glucose analog), but not when blocking GLUT. Glucose removal and the inhibition of glycolysis or oxidative phospholylation impaired the odor response. This evidence strongly suggests that glycolysis in the cilia and knob oxidative phosphorylation together fuel chemotransduction. SIGNIFICANCE STATEMENT How processes occurring in cilia and flagella are powered is a matter of general interest. Substantial progress has been made in unraveling the sensory transduction mechanisms, commonly occurring in such structures; however, the energy sources powering them have been scarcely explored. Accessibility to

  6. 24 CFR 401.403 - Rejection of a request for a Restructuring Plan because of actions or omissions of owner or...

    Science.gov (United States)

    2010-04-01

    ... Restructuring Plan because of actions or omissions of owner or affiliate or project condition. 401.403 Section... URBAN DEVELOPMENT MULTIFAMILY HOUSING MORTGAGE AND HOUSING ASSISTANCE RESTRUCTURING PROGRAM (MARK-TO... or omissions of owner or affiliate or project condition. (a) Ongoing determination of owner and...

  7. Re-structuring of a Dutch mono-industrial region; example of Twente : Hoofdstuk uit: The social and economic problems of monotowns

    NARCIS (Netherlands)

    Bazen, J.C. (Jacques); Bijleveld, P.C. (Paul)

    2012-01-01

    Re-structuring of a Dutch mono-industrial region; example of Twente Table of contents of the chapter Introduction Geography and location of Twente Industrialization of Twente and development of the Textile Industry Decline of the Textile Industry Restructuring Twente: arguments for a

  8. Community Restructuring and Depressive Symptoms of Rural Mature and Elderly Adults: A Multilevel Analysis Based on a National Dataset in China.

    Science.gov (United States)

    Yang, Fan; Lou, Vivian W Q

    2017-01-01

    Community restructuring is an important predictor for residents' mental health. However, few studies have investigated how it affects the depressive symptoms of rural ageing population. Using cross-sectional data from China Health and Retirement Longitudinal Study (CHARLS), this study examined how community restructuring was associated with depressive symptoms of Chinese rural mature and older adults and what community-level factors mediated the association. We found that people in restructuring communities reported .75 unit lower depression score (p < .01); community restructuring is associated with more infrastructure, recreational amenities, and grassroots organization (p < .001) in the community; and the availability of infrastructure (p < .01) and grassroots organization (p < .05) had significant indirect effect on the association between community restructuring and depressive symptoms. World countries' urbanization policy shall not only focus on community physical environment, but also on the development of grassroots organizations that involve and connect local people.

  9. Shear-induced network-to-network transition in a block copolymer melt

    International Nuclear Information System (INIS)

    Cochran, Eric W.; Bates, Frank S.

    2004-01-01

    A tricontinuous (10,3)c network phase is documented in a poly(cyclohexylethylene-b-ethylethylene-b-ethylene) triblock copolymer melt based on small-angle x-ray scattering. Application of shear transforms the self-assembled soft material into a single crystal (10,3)d network while preserving the short-range threefold connector geometry. Long-range topological restructuring reduces the space group symmetry, from Fddd to Pnna, maintaining orthorhombic lattice symmetry. Both phases are stable to long time annealing, indicative of nearly degenerate free energies and prohibitive kinetic barriers

  10. Health care restructuring and family physician care for those who died of cancer

    Directory of Open Access Journals (Sweden)

    Johnston Grace

    2005-01-01

    Full Text Available Abstract Background During the 1990s, health care restructuring in Nova Scotia resulted in downsized hospitals, reduced inpatient length of stay, capped physician incomes and restricted practice locations. Concurrently, the provincial homecare program was redeveloped and out-of-hospital cancer deaths increased from 20% (1992 to 30% (1998. These factors all pointed to a transfer of end-of-life inpatient hospital care to more community-based care. The purpose of this study was to describe the trends in the provision of Family Physician (FP visits to advanced cancer patients in Nova Scotia (NS during the years of health care restructuring. Methods Design Secondary multivariate analysis of linked population-based datafiles including the Queen Elizabeth II Health Sciences Centre Oncology Patient Information System (NS Cancer Registry, Vital Statistics, the NS Hospital Admissions/Separations file and the Medical Services Insurance Physician Services database. Setting Nova Scotia, an eastern Canadian province (population: 950,000. Subjects: All patients who died of lung, colorectal, breast or prostate cancer between April 1992 and March 1998 (N = 7,212. Outcome Measures Inpatient and ambulatory FP visits, ambulatory visits by location (office, home, long-term care facility, emergency department, time of day (regular hours, after hours, total length of inpatient hospital stay and number of hospital admissions during the last six months of life. Results In total, 139,641 visits were provided by family physicians: 15% of visits in the office, 10% in the home, 5% in the emergency department (ED, 5% in a long-term-care centre and 64% to hospital inpatients. There was no change in the rate of FP visits received for office, home and long-term care despite the fact that there were 13% fewer hospital admissions, and length of hospital stay declined by 21%. Age-sex adjusted estimates using negative binomial regression indicate a decline in hospital inpatient FP

  11. Curriculum restructuring in Higher Education after the Bologna Process: a new pedagogic regime?

    Directory of Open Access Journals (Sweden)

    Berit Karseth

    2006-01-01

    Full Text Available By adopting the Bologna Process as the frame of reference this article discusses the ongoing policy of curriculum restructuring in higher education.The analysis is based on policy documents produced on the European scene and documents which describe the restructuring process in the highereducation system of Norway. The article shows how the new discourse presented by the Bologna Process challenges the traditional curriculum discourses in higher education. The credit accumulation and transfer discourse asks for a curricular framework which enhances mobility,employability and competitiveness. Although the rhetoric calls attention to institutional autonomy the curriculum restructuring represents a standardisation of higher education whereby the management of credit transfer and accumulation becomes the salient task. The aim is to develop a highly reliable space of higher education which is manageable and predictable. Although a flexible curriculum approach together with a studentcentred pedagogy are advocated, the Bologna Process can be read as a rational programme that tends to create a discourse for the governing of the student (the self in light of the objectives already set.Tras adoptar el proceso de Bolonia como punto de referencia, este artículo se encarga de analizar la corriente política de reestructuración de currículos en educación superior. El análisis se basa en documentos producidos en el ámbito europeo, algunos dedicados al estudio del proceso de reestructuración de la educación superior en Noruega. El artículo muestra cómo el nuevo discurso presentado por el proceso de Bolonia, pone en entredicho los discursos tradicionales en educación superior en referencia al currículo. La acumulación y transferencia de créditos necesitan moverse en un marco más actual, que realce la movilidad, empleabilidad y competitividad. Aunque la retórica pueda ser importante en términos de autonomía institucional, la reestructuraci

  12. The Potential Economic Impact of Electricity Restructuring in the State of Oklahoma: Phase II Report; FINAL

    International Nuclear Information System (INIS)

    Hadley, SW

    2001-01-01

    Because of the recent experiences of several states undergoing restructuring (e.g., higher prices, greater volatility, lower reliability), concerns have been raised in states currently considering restructuring as to whether their systems are equally vulnerable. Factors such as local generation costs, transmission constraints, market concentration, and market design can all play a role in the success or failure of the market. These factors along with the mix of generation capacity supplying the state will influence the relative prices paid by consumers. The purpose of this project is to provide a model and process to evaluate the potential price and economic impacts of restructuring the Oklahoma electric industry. The Phase I report concentrated on providing an analysis of the Oklahoma system in the near-term, using only present generation resources and customer demands. This Phase II study analyzed the Oklahoma power market in 2010, incorporating the potential of new generation resources and customer responses. Five key findings of this Phase II were made: (1) Projected expansion in generating capacity exceeds by over 3,000 MW the demands within the state plus the amount that could be exported with the current transmission system. (2) Even with reduced new plant construction, most new plants could lose money (although residential consumers would see lower rates) unless they have sufficient market power to raise their prices without losing significant market share (Figure S-1). (3) If new plants can raise prices to stay profitable, existing low-cost coal and hydro plants will have very high profits. Average prices to customers could be 5% to 25% higher than regulated rates (Figure S-1). If the coal and hydro plants are priced at cost-based rates (through long-term contracts or continued regulation) while all other plants use market-based rates then prices are lower. (4) Customer response to real-time prices can lower the peak capacity requirements by around 9

  13. The bioenergetic and antioxidant status of neurons is controlled by continuous degradation of a key glycolytic enzyme by APC/C-Cdh1.

    Science.gov (United States)

    Herrero-Mendez, Angel; Almeida, Angeles; Fernández, Emilio; Maestre, Carolina; Moncada, Salvador; Bolaños, Juan P

    2009-06-01

    Neurons are known to have a lower glycolytic rate than astrocytes and when stressed they are unable to upregulate glycolysis because of low Pfkfb3 (6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-3) activity. This enzyme generates fructose-2,6-bisphosphate (F2,6P(2)), the most potent activator of 6-phosphofructo-1-kinase (Pfk1; ref. 4), a master regulator of glycolysis. Here, we show that Pfkfb3 is absent from neurons in the brain cortex and that Pfkfb3 in neurons is constantly subject to proteasomal degradation by the action of the E3 ubiquitin ligase, anaphase-promoting complex/cyclosome (APC/C)-Cdh1. By contrast, astrocytes have low APC/C-Cdh1 activity and therefore Pfkfb3 is present in these cells. Upregulation of Pfkfb3 by either inhibition of Cdh1 or overexpression of Pfkfb3 in neurons resulted in the activation of glycolysis. This, however, was accompanied by a marked decrease in the oxidation of glucose through the pentose phosphate pathway (a metabolic route involved in the regeneration of reduced glutathione) resulting in oxidative stress and apoptotic death. Thus, by actively downregulating glycolysis by APC/C-Cdh1, neurons use glucose to maintain their antioxidant status at the expense of its utilization for bioenergetic purposes.

  14. Osteoinduction of Umbilical Cord and Palate Periosteum-Derived Mesenchymal Stem Cells on Poly-Co-Glycolytic Acid Nano-Microfibers

    Science.gov (United States)

    Caballero, Montserrat; Pappa, Andrew; Roden, Katherine; Krochmal, Daniel J.; van Aalst, John A.

    2014-01-01

    The need for tissue engineered bone to treat complex craniofacial bone defects secondary to congenital anomalies, trauma, and cancer extirpation is sizeable. Traditional strategies for treatment have focused on autologous bone in younger patients and bone substitutes in older patients. However, the capacity for merging new technologies, including the creation of nano and microfiber scaffolds with advances in natal sources of stem cells, is crucial to improving our treatment options. The advantages of using smaller diameter fibers for scaffolding are two-fold: the similar fiber diameters mimic the in vivo extracellular matrix construct;, and smaller fibers also provide a dramatically increased surface area for cell-scaffold interactions. In this study, we compare the capacity for a polymer with Federal Drug Administration (FDA) approval for use in humans, poly-co-glycolytic acid (PLGA) from Delta polymer, to support osteoinduction of mesenchymal stem cells (MSCs) harvested from the umbilical cord (UC) and palate periosteum (PP). Proliferation of both UC- and PP-derived MSCs was improved on PLGA scaffolds. PLGA scaffolds promoted UC MSC differentiation (indicated by earlier gene expression and higher calcium deposition), but not in PP-derived MSCs. UC-derived MSCs on PLGA nano-micro-fiber scaffolds have potential clinical utility in providing solutions for craniofacial bone defects, with the added benefit of earlier availability. PMID:24691324

  15. Influence of exercise on the activity and the distribution between free and bound forms of glycolytic and associated enzymes in tissues of horse mackerel

    Directory of Open Access Journals (Sweden)

    Lushchak V.I.

    2001-01-01

    Full Text Available The effects of short-term burst (5 min at 1.8 m/s swimming and long-term cruiser (60 min at 1.2 m/s swimming on maximal enzyme activities and enzyme distribution between free and bound states were assessed for nine glycolytic and associated enzymes in tissues of horse mackerel, Trachurus mediterraneus ponticus. The effects of exercise were greatest in white muscle. The activities of phosphofructokinase (PFK, pyruvate kinase (PK, fructose-1,6-bisphosphatase (FBPase, and phosphoglucomutase (PGM all decreased to 47, 37, 37 and 67%, respectively, during 60-min exercise and all enzymes except phosphoglucoisomerase (PGI and PGM showed a change in the extent of binding to subcellular particulate fractions during exercise. In red muscle, exercise affected the activities of PGI, FBPase, PFK, and lactate dehydrogenase (LDH and altered percent binding of only PK and LDH. In liver, exercise increased the PK activity 2.3-fold and reduced PGI 1.7-fold only after 5 min of exercise but altered the percent binding of seven enzymes. Fewer effects were seen in brain, with changes in the activities of aldolase and PGM and in percent binding of hexokinase, PFK and PK. Changes in enzyme activities and in binding interactions with subcellular particulate matter appear to support the altered demands of tissue energy metabolism during exercise.

  16. [Contents of the glucose transporters GLUT1 and GLUT4 in oxidative and glycolytic muscles of goat kids and adult goats].

    Science.gov (United States)

    Dühlmeier, R; Voigt, J; Breves, G; Sallmann, H P

    2005-11-01

    The objective of this study was to elucidate, whether the impaired insulin sensitivity with regard to glucose utilisation in ruminating goats compared with suckling goat kids may be due to a reduced expression of the glucose transporters GLUT1 and GLUT4 in skeletal muscle. Muscle samples were removed from red, oxidative muscles (M. masseter, diaphragm) and white, glycolytic muscles (M. longissimus dorsi, and M. semitendinosus) of five goat kids fed with milk exchanger and nine adult goats of different stages of life. Samples were analysed for their GLUT1 and GLUT4 contents. The muscles were characterised metabolically by measuring the activities of isocitrate dehydrogenase (ICDH) and of lactate dehydrogenase. In all four analysed muscles the GLUT4 contents of adult goats were significantly (p kids. Significant differences concerning the GLUT4 contents in skeletal muscles were not detected in adult goats of different stages of life. The GLUT1 contents differed to a lower extend between goat kids and adult goats. The results of this investigation indicate that the impaired insulin sensitivity of adult compared with suckling ruminants is accompanied by or leads to a decreased GLUT4 expression.

  17. Restructuring and Mergers of the South African Post-Apartheid Tertiary System (1994-2011): A Critical Analysis

    Science.gov (United States)

    Mouton, Nelda; Louw, G. P.; Strydom, G. L.

    2013-01-01

    Socio-economic and vocational needs of communities, governments and individuals change over the years and these discourses served as a compass for restructuring of higher institutions in South Africa from 1994. Before 1994, the claim to legitimacy for government policies in higher education rested on meeting primarily the interests of the white…

  18. Separate and Combined Effects of Cue-Controlled Relaxation and Cognitive Restructuring in the Treatment of Musical Performance Anxiety.

    Science.gov (United States)

    Sweeney, Gladys Acevedo; Horan, John J.

    1982-01-01

    Music students with reactive and adaptive anxieties participated in a musical performance anxiety reduction program. Cue-controlled relaxation (CCR) and cognitive restructuring (CR) were examined separately and in combination in comparison with a standard treatment control condition. The CCR and CR treatments were each effective in reducing state…

  19. University Restructuring and the Reconfiguration of Faculty Members' Work Context in a Public State University in Mexico

    Science.gov (United States)

    Montero-Hernandez, Virginia; Levin, John S.

    2013-01-01

    This study describes the effects of neo-liberal restructuring for universities upon the reconfiguration of academics' work context in a public state university in Mexico. Findings show that implementation of the federal program titled Faculty Enhancement Program during the late 1990s created a separation between traditional and new academic…

  20. Enhancing Treatment for School-Age Children Who Stutter I. Reducing Negative Reactions through Desensitization and Cognitive Restructuring

    Science.gov (United States)

    Murphy, William P.; Yaruss, J. Scott; Quesal, Robert W.

    2007-01-01

    This paper describes several treatment strategies that clinicians can use to address negative affective, behavioral, and cognitive reactions that school-age children who stutter may experience as part of their disorder. Specific strategies include desensitization to stuttering, cognitive restructuring, self-acceptance, purposeful self-disclosure,…

  1. Comparative prediction of nonepileptic events using MMPI-2 clinical scales, Harris Lingoes subscales, and restructured clinical scales.

    Science.gov (United States)

    Yamout, Karim Z; Heinrichs, Robin J; Baade, Lyle E; Soetaert, Dana K; Liow, Kore K

    2017-03-01

    The Minnesota Multiphasic Personality Inventory-2 (MMPI-2) is a psychological testing tool used to measure psychological and personality constructs. The MMPI-2 has proven helpful in identifying individuals with nonepileptic events/nonepileptic seizures. However, the MMPI-2 has had some updates that enhanced its original scales. The aim of this article was to test the utility of updated MMPI-2 scales in predicting the likelihood of non-epileptic seizures in individuals admitted to an EEG video monitoring unit. We compared sensitivity, specificity, and likelihood ratios of traditional MMPI-2 Clinical Scales against more homogenous MMPI-2 Harris-Lingoes subscales and the newer Restructured Clinical (RC) scales. Our results showed that the Restructured Scales did not show significant improvement over the original Clinical scales. However, one Harris-Lingoes subscale (HL4 of Clinical Scale 3) did show improved predictive utility over the original Clinical scales as well as over the newer Restructured Clinical scales. Our study suggests that the predictive utility of the MMPI-2 can be improved using already existing scales. This is particularly useful for those practitioners who are not invested in switching over to the newly developed MMPI-2 Restructured Form (MMPI-2 RF). Copyright © 2016 Elsevier Inc. All rights reserved.

  2. A Key to Organizations Working in: Employee Training, Labor-Management Relations, Work Restructuring, Workplace Literacy. Workforce Tools. Resource Guide.

    Science.gov (United States)

    National Alliance of Business, Inc., Washington, DC.

    This guide is intended as a resource for service providers and small and midsized companies seeking assistance in the areas of employer training, labor-management relations, work restructuring, and workplace literacy. It contains annotated listings of 194 national and state organizations and agencies conducting work in the four areas. Among the…

  3. Electric industry restructuring and environmental issues: A comparative analysis of the experience in California, New York, and Wisconsin

    Energy Technology Data Exchange (ETDEWEB)

    Fang, J.M.; Galen, P.S.

    1996-08-01

    Since the California Public Utilities Commission (CPUC) issued its April 20, 1994, Blue Book proposal to restructure the regulation of electric utilities in California to allow more competition, over 40 states have initiated similar activities. The question of how major public policy objectives such as environmental protection, energy efficiency, renewable energy, and assistance to low-income customers can be sustained in the new competitive environment is also an important element being considered. Because many other states will undergo restructuring in the future, the experience of the {open_quotes}early adopter{close_quotes} states in addressing public policy objectives in their electric service industry restructuring processes can provide useful information to other states. The Competitive Resource Strategies Program of the U.S. Department of Energy`s (DOE`s) Office of Utility Technologies, is interested in documenting and disseminating the experience of the pioneering states. The Center for Energy Analysis and Applications of the National Renewable Energy Laboratory assisted the Office of Utility Technologies in this effort with a project on the treatment of environmental issues in electric industry restructuring.

  4. Students' Understanding of the Nature of Matter and Chemical Reactions--A Longitudinal Study of Conceptual Restructuring

    Science.gov (United States)

    Øyehaug, Anne Bergliot; Holt, Anne

    2013-01-01

    This longitudinal study aims to provide greater insight into how students' understanding of matter and chemical reactions develops over time and how their knowledge structures are restructured. Four case-study students in a Norwegian primary school were followed for two years from age 10-11 to age 12-13. Researchers were responsible for…

  5. Japan's Ministry of Education "Becoming the Right": Neo-Liberal Restructuring and the Ministry's Struggles for Political Legitimacy

    Science.gov (United States)

    Takayama, Keita

    2008-01-01

    This article first examines the controversial revision of the Fundamental Law of Education (FLE) by situating it in the larger global context of neo-liberal and neo-conservative state-restructuring and education reform. It then focuses on the domestic politics behind what seems to be the global convergence of education policy along neo-liberal and…

  6. Down-scaling of bituminous coal mining, restructuring of steel works and heavy engineering in the Ostrava region

    Czech Academy of Sciences Publication Activity Database

    Klusáček, Petr

    2005-01-01

    Roč. 13, č. 2 (2005), s. 3-12 ISSN 1210-8812 R&D Projects: GA AV ČR(CZ) IBS3086005 Institutional research plan: CEZ:AV0Z30860518 Keywords : Ostrava region * restructuring * steemaking and heavy industry * downscaling of coal- mining Subject RIV: DE - Earth Magnetism, Geodesy, Geography

  7. THE RESTRUCTURING OF ROMANIAN POWER SECTOR AT THE CROSSROADS: COMPETITIVE MARKETS OR NEO-COLBERTISM?

    Directory of Open Access Journals (Sweden)

    Oana Diaconu

    2007-12-01

    Full Text Available Despite efforts made by European Commission to liberalize electricity markets and foster integration, there are still significant barriers to free competition. Until now, Romania was one of the countries that have been compliant to the European Union’s electricity directives, being ahead of several older member states in this area. However, reforms have not started to pay out, suggesting that the model of combining state-owned non-competing generators with private/privatized distributors and suppliers may not be the best model of market deregulation. As a result, Romanian authorities have started to talk about plans to restructure the sector, by re-consolidating the unbundled generation companies and the state-owned distribution companies into one national energy company, aiming to create a national champion, competitive on the regional markets. However, these proposals are based on questionable economics and their adoption will have negative effects on market competition and, thus, on consumers.

  8. Behavior restructuring and social activation as self-control strategies facing alcohol consumption in adults

    Directory of Open Access Journals (Sweden)

    Diana Obando-Posada

    2013-06-01

    Full Text Available Alcohol consumption remains a world major problem because it affects more than a half of the world population and it is associated with multiple physical and psychological illnesses, domestic violence, traffic accidents, financial, labor and academic problems, among others. The purpose of this study is to assess the effect of behavior restructuring and social activation as self-control techniques, in three male adults of 21, 39 and 42 years old, who reported problems related with substance use. An intrasubject temporary series A-B-A-BC design was conducted. Results indicate that intervention aimed to modify behavioral patterns based on the suggested techniques, had a positive effect. In monitoring phase, all participants showed a decrease in consumed grams of alcohol and invested time on consumption, compared with the assessment phase.

  9. [Productive restructuring and its impact on labor relations in the public health services in Brazil].

    Science.gov (United States)

    Martins, Maria Inês Carsalade; Molinaro, Alex

    2013-06-01

    The restructuring of productive systems and economic globalization are directly impacting the basic social rights of workers. In the semi-peripheral countries such as Brazil, where the wage-based society and the consolidation of social rights are not completely implemented, this process of change in the world of labor contributes to aggravate the inequality in the capital-labor relationship and hampers access to employment. By means of a critical review of the scientific literature regarding changes in the world of labor and its impact on the organization and production of health services in Brazil, this article pinpoints the weakness of regulation of the labor market in Brazil, especially in the health sector. It also stresses the need to increase the debate on new forms of institutionalization of the labor relationship in order to ensure equity in the workplace and protect the rights to work and in the workplace.

  10. Considerations on socio-economic consequences of restructuring of military industry in Italy

    International Nuclear Information System (INIS)

    Traballesi, A.

    1994-01-01

    In Italy there has been no brain drain nor scientific transfer from the military to the civil sector, from large public corporations to private ones, even of small and medium size. What is happening now is the reduction by military enterprises of unqualified labour, while research and development functions are maintained as well as technical executive staff, according to the assumption that all process potentials can be used in the future and that only production levels are reduced. This is only a transition phase, brought up by the restructuring process, in the future a new balanced situation will arise. It will be characterised by a more efficient and effective interchange among universities, research centres and military enterprises, furthermore the civilian scientific technological basis will acquire more strategic value than that of the military basis, quite opposite of what has been the case so far and following the modifications of the economic situation and international politics

  11. IPO AS AN EFFECTIVE INSTITUTION AND A PRECONDITION OF CORPORATE RESTRUCTURING FOR AGRICULTURAL COMPANIES

    Directory of Open Access Journals (Sweden)

    D. Baiura

    2015-06-01

    Full Text Available The key factors of investment attractiveness of the agricultural sector of Ukraine and the need to finance its development through the use of the IPO are analyzed. The main advantages and disadvantages received by any company after passing the IPO are demonstrated. The analysis of the results of the IPO of agricultural enterprises of Ukraine is conducted. Such areas of corporate restructuring as organization of a new corporate structure, bringing system of a corporate governance to the principles of OECD, increased transparency of financial – economic and not financial reporting, optimization of financial management and business process, and also appropriate measures for each of these areas in order to ensure an effectiveness of the IPO are proposed.

  12. Can rural electric cooperatives survive in a restructured US electric market? An empirical analysis

    International Nuclear Information System (INIS)

    Greer, Monica Lynne

    2003-01-01

    This paper examines the ability of rural electric distribution cooperatives to continue operating in their present form in a restructured electricity market. More specifically, I develop and estimate a quadratic cost model, which, unlike many of the cost functions employed in studies of this nature, conforms to all of the properties of a proper cost function. Using 1996 data, I find that these firms are not operating in a cost-minimizing fashion. This finding seems to occur because each is too small in terms of the quantity of electricity distributed. As a result, mergers between these firms could yield substantial savings and help ensure their survival in their present form in a deregulated market

  13. The Effect of Economic and Social Restructuring on the Towns of Nehoiu and Pogoanele

    Directory of Open Access Journals (Sweden)

    ANA ANDUŢA PUNCIOIU

    2012-01-01

    Full Text Available Since early 1989, the small settlements of Nehoiu and Pogoanele, located in the south-eastern part of Buzău County and declared towns in the same year, entered a new stage of evolution.After a long period of evolution under socialism, people and especially authorities decided to adjust the evolution and the development of settlements. This change did not only have repercussions on the entire economic base but also on the population, imprinting a descendent evolution. Economic restructuring is felt on the quality of life, purchasing power is increasingly smaller, many families are poor, with the possibility of living only from one day to another. Industry and agriculture have suffered the biggest change in the sense that the labour force in these two areas has steadily decreased. Current development opportunities include: increasing the share of active population in the tertiary sector and attracting investors from the private sector.

  14. The prerequisite for competition in the restructured wholesale Saudi electricity market

    International Nuclear Information System (INIS)

    Al-Muhawesh, Tareq A.; Qamber, Isa S.

    2008-01-01

    Protection of customers against monopoly is the first and main objective of the Saudi Electricity and Co-generation Regulatory Authority (ECRA). The second important objective, as recommended by the present study, is regulating natural monopoly businesses [Saudi electricity national grid (SENG) and Saudi electricity distribution (SED)] in addition to promoting real competition in competitive businesses [power supply providers (PSPs) and customer service providers (CSPs)]. Another four main objectives of ECRA are to promote the efficient use of energy and natural resources, to ensure a reasonable rate of return for PSPs and CSPs and at the same time to be fair to end-users, to ensure reasonable charges to SENG and SED services to be adequate for them to run the organization in a break-even manner and to maintain the system's security and reliability. The present paper discusses the way to improve and restructure the Saudi electricity market. (author)

  15. Eurozone’s Future: Convergence, Restructuring or “Muddling Through”?

    Directory of Open Access Journals (Sweden)

    Antonin Rusek

    2014-12-01

    Full Text Available The mixture of the recent EU policies – mainly the “fiscal compact” and the OMT approach by the ECB – restored the nominal stability for the Eurozone and therefore preserved the common currency. However, the same steps resulted in a widening gap between the Northern tier and the Mediterranean countries – the real divergence. To address this issue – which is potentially a mortal threat to the Eurozone in its present configuration – the three policy options are available: A restoration of a real convergence, the Eurozone‟s restructuring or a “muddling through”. The first two require radical policy steps which in the present political and economic climate are very unlikely. That leaves “muddling through” as the likeliest future approach. However, its inherent ad hoc nature breeds uncertainty, which, combined with a complacency stemming from the recent “successes” increases the possibility of a destructive future dynamics.

  16. Corporate restructuring of the global energy industry, the next steps: the case of gas in Europe

    International Nuclear Information System (INIS)

    Roland, K.; Soerensen, E.S.

    2000-01-01

    The European energy sector will undergo a wave of restructuring and reorganization in the next 12 to 36 months. Deregulation at both the EU and national levels provides a catalyst, creating a range of new commercial forces that will require actors fundamentally to reappraise their business strategies. Companies will look for efficiency gains, economies of scale and scope, new approaches to risk management and a strategic positioning in the pan-European market. This will result in far-reaching structural changes in the industry, leading to a small number of large, vertically integrated energy companies with a wide spread of geographical interests. In this paper, we analyse these trends with reference to the European gas industry. (orig.)

  17. Analytic study of the capital restructuring processes in metallurgical enterprises around the world and in Poland

    Directory of Open Access Journals (Sweden)

    B. Gajdzik

    2012-04-01

    Full Text Available The article presents the most common directions of changes in capital groups in the sector of metallurgy around the world and in Poland. During the observation of the restructuring activities in metallurgy the attention was turned to the integration of the capital groups, capital outsourcing and contract outsourcing, creation and the takeover of the dependent companies. The meaning of the capital consolidation was underlined on the global metallurgy market in order to gain competitive advantage through the increase of manufacturing capacity. The overview of the rating of the global steel producers in years 1989-2009 was conducted with this article in mind and it has shown the changes in the participation of particular capital groups in the world production of steel.

  18. Restructured fish product from white croacker (Micropogonias furnieri mince using microbial transglutaminas

    Directory of Open Access Journals (Sweden)

    Alex Augusto Gonçalves

    2010-08-01

    Full Text Available This study aimed at determining the influence of three concentrations of commercial transglutaminase enzyme in restructured fillet of minced fish from white croacker (Micropogonias furnieri, one of the four marine species with notability in Brazil. The restructured fillet developed had advantages when compared to traditional fillet, such as absence of spine and less flavour intensity (washes cycles. Washing process for white croacker mince was compared with five clarification agents: water (control, phosphoric acid (H3PO4, sodium chloride (NaCl, calcium carbonate (CaCO3 and sodium bicarbonate (NaHCO3. The higher quality product (whiteness was obtained with calcium carbonate washes. Three concentrations (1.5, 1.0 and 0.5% of microbial transglutaminase MGTase (Active TG-B %v/v and Active TG-BP %w/w were compared, in order to produce fish restructured product (boneless fillet. The concentration of 1.5% (both enzymes, produced better results. The restructured products were compared by sensory analysis and showed better sensory parameters (appearance, odour, flavour and texture samples treated with Active TG-B (solution form.Este estudo teve como objetivo determinar a influência de três concentrações de enzima transglutaminase comercial em filés reestruturado a partir de polpa de corvina (Micropogonias furnieri, uma das quatro espécies marinhas notáveis no Brasil. O filé reestruturado desenvolvido tem vantagens quando comparado aos filés tradicionais, tais como, a ausência da espinhas e sabor menos intenso (ciclos de lavagens. O processo de lavagem da polpa de corvina foi comparado com cinco agentes clarificantes: água (controle, ácido fosfórico (H3PO4, cloreto de sódio (NaCl, carbonato de cálcio (CaCO3 e bicarbonato de sódio (NaHCO3. O produto de qualidade superior (mais branco foi obtido com a lavagem com carbonato de cálcio. Três concentrações (1,5%, 1,0% e 0,5% de transglutaminase microbiana (Activa TG-B % v/v e Activa TG

  19. Performance des banques publiques et restructuration du secteur bancaire en Algérie

    OpenAIRE

    AMRANI, Mohamed

    2015-01-01

    Au cours des vingt dernières années, l'industrie bancaire a été le témoin d’une vague sans précédent de restructuration. Dans différents pays ces dernières ont été au coeur de la transition pour la mise en place de secteurs bancaires efficaces et performants. La thèse analyse la transformation du secteur bancaire en Algérie, le seul canal de financement de l'économie. Et donc la question du rôle de ce secteur est d'une extrême importance. Après avoir discuté des aspects théoriques des rest...

  20. Deregulation and restructuring of the power industry in the United States

    International Nuclear Information System (INIS)

    Bernardini, O.

    2001-01-01

    The article addresses the causal relations between deregulation and restructuring of the power sector in the United States during the last two decades. To better appreciate the issues involved in this country, the analysis starts with an account of the origins of the regulatory process at the local, state and federal levels of government. The article proceeds with a discussion of the factors which determined the partial liberalisation of electricity generation from the end of the 1970s and the subsequent extensive liberalisation during the course of the 1990s, resulting in the functional separation between generation, transmission and ancillary services. It concludes with a description of the radical changes currently underway in the power sector [it

  1. Restructuring higher education. Ideological Postmodernism, liberal professionalization and educational deregulated market

    Directory of Open Access Journals (Sweden)

    Mario Domínguez Sánchez

    2013-03-01

    Full Text Available The key of the implied model of the present educational restructuring is based on the correlation between a postmodern epistemology which questions all of the epistemological roots, the new management of the organizational culture, the reconceptualization of the educational work by means of a new professionalization and at least, by the discourse of the quality and excellence which proportionate an ideological covering for all this process. The funding of the demand which has transformed the Universities in PYME's, which are forced to reduce its costs in order to achieve the self-funding and even to obtain benefits, it closes in neoliberal terms the location of higher universities in an economical model of knowledge which is situated outside of the educational institutions.

  2. Impact of Credit Restructuring on the Quality of Bank Asset Portfolio. A Cluster Analysis Approach

    Directory of Open Access Journals (Sweden)

    Nicolae Dardac

    2011-06-01

    Full Text Available In this paper we proposed an analysis of the financial crisis impact on the procedures formanagement of loan portfolios in several banking systems. Despite ample liquidity injectionprograms implemented by major central banks and government actions, credit risk remains a keychallenge of the current banking systems. On a medium term, the high percentage of bad loans hasbecome a structural vulnerability. To maintain an acceptable quality of loan portfolios and not todamage the prudential and profitability indicators, credit institutions in EU member states haveproceeded to apply various techniques for credit restructuring. The quantitative analysis carried out inthe last part of the paper revealed a relatively moderate granularity of banking systems considered, interms of capitalization, volume of bank reserves and net provisions, in response to the persistent trendof loan portfolio deterioration.

  3. Industrial and institutional restructuring of the Russian electricity sector: Status and issues

    International Nuclear Information System (INIS)

    Engoian, Alda

    2006-01-01

    The reform of the Russian electricity industry represents one of the largest and most technically complex post-Soviet era industrial restructurings. This paper presents the framework, status, and perspectives for the Russian electricity sector liberalisation. Uncertainties regarding the effective implementation of the reform are specifically examined. Ultimately the critical analysis of the reform questions the feasibility and adequacy of the recommended measures in the specific Russian context. Current theories fail to answer a fundamental issue, namely how to promote investment in an obsolete electrical infrastructure and, more generally, whether a pure free market model is compatible with physical constraints posed by the electricity sector. A careless deregulation of the Russian electricity system could hinder the country's stable and sustainable development, as its economy and the population's service have traditionally been closely linked to the electricity industry

  4. Industrial and institutional restructuring of the Russian electricity sector: status and issues

    International Nuclear Information System (INIS)

    Engoian, A.

    2006-01-01

    The reform of the Russian electricity industry represents one of the largest and most technically complex post-Soviet era industrial restructurings. This paper presents the framework, status, and perspectives for the Russian electricity sector liberalisation. Uncertainties regarding the effective implementation of the reform are specifically examined. Ultimately the critical analysis of the reform questions the feasibility and adequacy of the recommended measures in the specific Russian context. Current theories fail to answer a fundamental issue, namely how to promote investment in an obsolete electrical infrastructure and, more generally, whether a pure free market model is compatible with physical constraints posed by the electricity sector. A careless deregulation of the Russian electricity system could hinder the country's stable and sustainable development, as its economy and the population's service have traditionally been closely linked to the electricity industry. (author)

  5. Restructuring of Holocentric Centromeres During Meiosis in the Plant Rhynchospora pubera.

    Science.gov (United States)

    Marques, André; Schubert, Veit; Houben, Andreas; Pedrosa-Harand, Andrea

    2016-10-01

    Centromeres are responsible for the correct segregation of chromosomes during mitosis and meiosis. Holocentric chromosomes, characterized by multiple centromere units along each chromatid, have particular adaptations to ensure regular disjunction during meiosis. Here we show by detecting CENH3, CENP-C, tubulin, and centromeric repeats that holocentromeres may be organized differently in mitosis and meiosis of Rhynchospora pubera Contrasting to the mitotic linear holocentromere organization, meiotic centromeres show several clusters of centromere units (cluster-holocentromeres) during meiosis I. They accumulate along the poleward surface of bivalents where spindle fibers perpendicularly attach. During meiosis II, the cluster-holocentromeres are mostly present in the midregion of each chromatid. A linear holocentromere organization is restored after meiosis during pollen mitosis. Thus, a not yet described case of a cluster-holocentromere organization, showing a clear centromere restructuration between mitosis and meiosis, was identified in a holocentric organism. Copyright © 2016 by the Genetics Society of America.

  6. Day surgery: Results after restructuration of a university public general surgery unit.

    Science.gov (United States)

    Gaucher, S; Cappiello, F; Bouam, S; Damardji, I; Aissat, A; Boutron, I; Béthoux, J P

    2013-06-01

    Nowadays, in France, development of the ambulatory surgery has stalled. This is probably related to the fact that ambulatory surgery is restricted by the law to the "day surgery" in 12 hours, and only 17 procedures are referenced for this surgery. Thus, conventional hospitalization remained the rule after surgery. In January 2010, our university general surgery unit was restructured. It evolved from a conventional unit to a predominantly ambulatory unit. Otherwise, our unit adjoins a hotel, even inside our institution, which accommodates patients, patient visitors and tourists. The aim of this retrospective study was to compare the postoperative accommodation modalities between two groups of patients. The first group consisted of patients admitted before January 2010, at the time of conventional activity, whereas the second group consisted of patients admitted after January 2010 in a restructured unit. Inclusion of patients admitted from April 1, 2008 to March 31, 2009 (conventional hospitalization period) and from April 1, 2010 to March 31, 2011 (ambulatory management period), scheduled for one single surgical procedure excluding emergency. A total of 360 patients were retained: 229 for the conventional period and 131 for the ambulatory period, with a median age of 55 (range 15-87). No statistically significant difference was noted between the two groups as concerned median age, gender or ASA status. The number of postoperative nights varied significantly between the two groups with a mean of 3.8 nights (median three nights, range 0-32) for the conventional period versus 0.4 nights (median 0 night, range 0-10) for the ambulatory period (Plegal period of 12 hours to 24 hours in order to expand the list of the referenced procedures. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  7. Effects of Inulin and Sodium Carbonate in Phosphate-Free Restructured Poultry Steaks

    Science.gov (United States)

    Öztürk, B.; Serdaroğlu, M.

    2017-09-01

    Recently inorganic phosphates used in meat product formulations have caused negative impact on consumers due to their potential health risks. Therefore, utilization of natural ingredients as phosphate replacers has come into prominence as a novel research topic to meet consumer demands for clean-label trends. In this study, we objected to investigate the effects of inulin utilization either in the powder or gelled form, alone or in combination with sodium carbonate on quality of phosphate-free restructured chicken steaks. Total moisture, protein, lipid and ash values of the trial groups were in the range of 71.54-75.46%, 22.60-24.31%, 0.94-1.70% and 1.45-2.13%, respectively. pH of the samples was between 6.18-6.39, significant increments were recorded in samples containing inulin with sodium carbonate. L*, a* and b* values were recorded as 78.92-81.05, 1.76-3.05 and 10.80-11.94, respectively, where use of gelled inulin resulted in changes of L* and a* values. Utilization of inulin in combination with sodium carbonate decreased cook loss and enhanced product yield. Sensory scores in control group with phosphate showed a similar pattern to sensory scores in groups with inulin and sodium carbonate. During storage, purge loss and lipid oxidation rate were similar in control and inulin + sodium carbonate samples. The results showed that use of inulin in combination with sodium carbonate provided equivalent physical, chemical and sensory quality to phosphates in restructured chicken steaks.

  8. Healthcare restructuring and hierarchical alignment: why do staff and managers perceive change outcomes differently?

    Science.gov (United States)

    Walston, Stephen L; Chou, Ann F

    2006-09-01

    Healthcare organizations have undergone major change efforts in the past decade. Sustained change is related to continued alignment among organizational participants and may fail with incongruent perceptions of change. This study identifies factors contributing to the alignment in perceptions of organizational change outcomes between executives and all other employees. The sample included 10 hospitals with survey responses from 421 executives and other employees. Using hierarchical linear modeling (HLM), perceptual alignment was modeled at the first level as a function of goal commitment, goal clarity, goal acceptance, goal specificity, staff participation, available skill set, and knowledge and at the second level as organizational size. Descriptive statistics showed employee perception of outcomes differed among personnel levels. HLM results showed that goal specificity, appropriate staff training, reward incentives, effective communication, information sharing, and organization's ability to sustain changes induced perceptual alignment in change outcomes. Staff involvement in designing change efforts increased perceptual misalignment, whereas involvement during implementation and maintenance phases increased alignment. This research uses cross-sectional data from 10 hospitals. Data were gathered from surveys that may have recall bias as hospitals were surveyed at different times after the implementation of their restructuring. Our findings enhance the understanding of processes and mechanisms that enable healthcare organizations to align organizational participants' efforts during change. Results suggest that decision-makers should create incentives to encourage innovative practices, institute effective communication mechanisms, selectively disseminate information, and involve participants in implementing and maintaining changes to achieve intended outcomes. ORIGINALITY/VALUE OF ARTICLE: This article provides unique insight into the importance and causes of

  9. Electricity and gas : market and price convergence : fundamentals of restructuring and convergence

    International Nuclear Information System (INIS)

    Heintz, H.; Spragins, R.

    2000-01-01

    One of the results of the transition from regulation to competition in the Canadian and American natural gas and electricity industries is convergence of the two industries. Convergence is occurring in the areas of corporate structuring activities (mergers and acquisitions), natural gas and electricity prices, products and services, and on a geographic basis. This study examines the restructuring and convergence from the perspective of industry stakeholders, consumers, competitors and regulators. The trend to deregulate to establish competitive markets has been driven by the assumption that lower prices and more choices will result. Deregulation has been made easier by technological developments and innovations in the area of conventional generation, distributed generation, information management and analysis, as well as mass communication channels such as the Internet. These changes have made it possible to measure and monitor energy use in real-time. Technological changes will continue to influence the energy industry. The use of different restructuring rules and regulations in jurisdictions that are implementing change may be one of the primary factors that could limit the extent of convergence. Successful competition by energy service providers in converged retail energy markets will depend on several factors, the first of which is the ability to control the customer interface through retail cycle services such as metering and billing. The second is the successful branding of corporate identities, products and services. These will ensure customer loyalty and facilitate the marketing of new products. Another factor would be the effective management of information regarding natural gas and electricity consumption patterns and the establishment of low cost operations through the use of conventional generation technologies. The final factor for successful competition is the effective use of low cost communication technologies such as the Internet. The transition

  10. A restructuring proposal based on MELCOR for severe accident analysis code development

    Energy Technology Data Exchange (ETDEWEB)

    Park, Sun Hee; Song, Y. M.; Kim, D. H. [Korea Atomic Energy Research Institute, Taejeon (Korea)

    2000-03-01

    In order to develop a template based on existing MELCOR code, current data saving and transferring methods used in MELCOR are addressed first. Then a naming convention for the constructed module is suggested and an automatic program to convert old variables into new derived type variables has been developed. Finally, a restructured module for the SPR package has been developed to be applied to MELCOR. The current MELCOR code ensures a fixed-size storage for four different data types, and manages the variable-sized data within the storage limit by storing the data on the stacked packages. It uses pointer to identify the variables between the packages. This technique causes a difficult grasping of the meaning of the variables as well as memory waste. New features of FORTRAN90, however, make it possible to allocate the storage dynamically, and to use the user-defined data type which lead to a restructured module development for the SPR package. An efficient memory treatment and as easy understanding of the code are allowed in this developed module. The validation of the template has been done by comparing the results of the modified code with those from the existing code, and it is confirmed that the results are the same. The template for the SPR package suggested in this report hints the extension of the template to the entire code. It is expected that the template will accelerate the code domestication thanks to direct understanding of each variable and easy implementation of modified or newly developed models. 3 refs., 15 figs., 16 tabs. (Author)

  11. Restructured graphene sheets embedded carbon film by oxygen plasma etching and its tribological properties

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Meiling [Key Laboratory of Education Ministry for Modern Design and Rotor-Bearing System, School of Mechanical Engineering, Xi’an Jiaotong University, Xi’an 710049 (China); Diao, Dongfeng, E-mail: dfdiao@szu.edu.cn [Institute of Nanosurface Science and Engineering (INSE), Shenzhen University, Shenzhen 518060 (China); Yang, Lei [Key Laboratory of Education Ministry for Modern Design and Rotor-Bearing System, School of Mechanical Engineering, Xi’an Jiaotong University, Xi’an 710049 (China); Fan, Xue [Institute of Nanosurface Science and Engineering (INSE), Shenzhen University, Shenzhen 518060 (China)

    2015-12-01

    Highlights: • Oxygen plasma etching was developed to improve tribological properties of GSEC film. • Etching restructured 3 nm top layer with smaller crystallite size and higher sp{sup 3} fraction. • The etched film had smoother surface, enhanced mechanical properties, longer wear life. • High electrical conductivity and strong magnetism were retained after etching. - Abstract: An oxygen plasma etching technique was introduced for improving the tribological properties of the graphene sheets embedded carbon (GSEC) film in electron cyclotron resonance plasma processing system. The nanostructural changing in the film caused by oxygen plasma etching was examined by transmission electron microscope, Raman spectroscopy and X-ray photoelectron spectroscopy, showing that the 3 nm thick top surface layer was restructured with smaller graphene nanocrystallite size as well as higher sp{sup 3} bond fraction. The surface roughness, mechanical behavior and tribological properties of the original GSEC and oxygen plasma treated GSEC films were compared. The results indicated that after the oxygen plasma treatment, the average roughness decreased from 20.8 ± 1.1 nm to 1.9 ± 0.1 nm, the hardness increased from 2.3 ± 0.1 GPa to 2.9 ± 0.1 GPa, the nanoscratch depth decreased from 64.5 ± 5.4 nm to 9.9 ± 0.9 nm, and the wear life increased from 930 ± 390 cycles to more than 15,000 frictional cycles. The origin of the improved tribological behavior was ascribed to the 3 nm thick graphene nanocrystallite film. This finding can be expected for wide applications in nanoscale surface engineering.

  12. Nucleosome dynamics: HMGB1 facilitates nucleosome restructuring and collaborates in estrogen-responsive gene expression

    Directory of Open Access Journals (Sweden)

    William M. Scovell

    2016-12-01

    Full Text Available The genome in the human cell is extraordinarily compacted in the nucleus. As a result, much of the DNA is inaccessible and functionally inert. Notwithstanding the highly efficient packaging, mechanisms have evolved to render DNA sites accessible that then enable a multitude of factors to carry out ongoing and vital functions. The compaction is derived from DNA complexation within nucleosomes, which can further consolidate into a higher-order chromatin structure. The nucleosome and nucleosomal DNA are not static in nature, but are dynamic, undergoing structural and functional changes as the cell responds to stresses and/or metabolic or environmental cues. We are only beginning to understand the forces and the complexes that engage the nucleosome to unearth the tightly bound and inaccessible DNA sequences and provide an opening to more accessible target sites. In many cases, current findings support a major role for the action of ATP-dependent chromatin remodeling complexes (CRCs in providing an avenue to factor accessibility that leads to the activation of transcription. The estrogen receptor α (ERα does not bind to the estrogen response element (ERE in the canonical nucleosome. However, evidence will be presented that HMGB1 restructures the nucleosome in an ATP-independent manner and also facilitates access and strong binding of ERα to ERE. The features that appear important in the mechanism of action for HMGB1 will be highlighted, in addition to the characteristic features of the restructured nucleosome. These findings, together with previous evidence, suggest a collaborative role for HMGB1 in the step-wise transcription of estrogen-responsive genes. In addition, alternate mechanistic pathways will be discussed, with consideration that “HMGB1 restructuring” of the nucleosome may generally be viewed as a perturbation of the equilibrium of an ensemble of nearly isoenergetic nucleosome states in an energy landscape that is driven by

  13. Restructuring American health care financing: first of all, do no harm!

    Science.gov (United States)

    Berk, P D

    1993-07-01

    Health care costs are climbing throughout the western world. Aging populations and the costs of advanced technology are the principal forces behind much of this global increase. No country has yet succeeded in containing these growing costs other than by some form of rationing. A variety of experimental strategies, including managed competition, are being considered or tested, but none is clearly effective. American health care expenditures differ, not in that they are rising, but in their enormously high starting point. Among other things, our higher costs reflect administrative costs of more than 20%, double those of Canada and nearly triple the European average; a malpractice system that, whatever its possible advantages, costs more than 10 times as much as it pays out to the injured; the enormous medical costs of poverty; maldistribution of physician specialties and incomes; and reimbursement systems that eliminate consumer input and oversight. Restructuring the system of health care financing to bring administrative costs in line with those of other nations could save at least $70 billion annually; another $25 billion or more could be saved by replacing the malpractice system with more cost-effective alternatives. These savings could defray the costs of insuring all those not now covered, without increasing either costs to the middle class, through taxation of benefits, or total health care expenditures. With all Americans covered, the necessary restructuring of the system of health care delivery could be conducted without the current pressure for immediate drastic reform, which carries with it the risk of serious error. In dealing with the sick, physicians are taught to apply two maxims: "primum non nocere" or "first of all, do no harm!"; and the rule of therapeutic restraint. The latter states that a severe chronic illness may respond better, and with fewer complications, to gradual corrective measures than to highly aggressive therapy. Both rules could well

  14. Comparative Effects of Phosphoenolpyruvate, a Glycolytic Intermediate, as an Organ Preservation Agent with Glucose and N-Acetylcysteine against Organ Damage during Cold Storage of Mouse Liver and Kidney

    OpenAIRE

    Ishitsuka, Yoichi; Fukumoto, Yusuke; Kondo, Yuki; Irikura, Mitsuru; Kadowaki, Daisuke; Narita, Yuki; Hirata, Sumio; Moriuchi, Hiroshi; Maruyama, Toru; Hamasaki, Naotaka; Irie, Tetsumi

    2013-01-01

    We evaluated the usefulness of phosphoenolpyruvate (PEP), a glycolytic intermediate with antioxidative and energy supplementation potentials, as an organ preservation agent. Using ex vivo mouse liver and kidney of a static cold storage model, we compared the effects of PEP against organ damage and oxidative stress during cold preservation with those of glucose or N-acetylcysteine (NAC). Lactate dehydrogenase (LDH) leakage, histological changes, and oxidative stress parameters (measured as thi...

  15. Clean Restructuring: Design Elements for Low-Carbon Wholesale Markets and Beyond. A 21st Century Power Partnership Thought Leadership Report

    Energy Technology Data Exchange (ETDEWEB)

    Shah, Monisha [National Renewable Energy Lab. (NREL), Golden, CO (United States); Valenzuela, Jose Maria [World Wildlife Fund, Mexico DF (Mexico); Mora, Hector Alejandro Beltran [Energy Regulatory Commission of Mexico, Mexico DF (United States); Moller Porst, Kim [Danish Energy Agency, Copenhagen (Denmark); Hasselager, Anders [Danish Energy Agency, Copenhagen (Denmark); Friis-Jensen, Sandra [Danish Energy Agency, Copenhagen (Denmark); Vingaard, Mette [Danish Energy Agency, Copenhagen (Denmark); Wigand, Fabian [Ecofys, London (England); Tiedemann, Silvana [Ecofys, London (England); Bird, Lori [National Renewable Energy Lab. (NREL), Golden, CO (United States); Zinaman, Owen [National Renewable Energy Lab. (NREL), Golden, CO (United States); Logan, Jeffrey [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-05-01

    Countries around the world are in various stages of reforming and restructuring their power systems to better meet development needs and decarbonization commitments. Changes in technology, business models, societal needs, and environmental goals are increasing pressure on countries to consider improvements to their power systems. This report addresses key issues associated with clean restructuring--the transition from traditional, vertically integrated utilities to competitive wholesale markets that rely increasingly on variable renewable electricity sources, demand response, and other clean energy options. The report also includes case studies from Mexico, Denmark, and Germany to provide real-world examples of clean restructuring from different perspectives.

  16. Glycolytic Functions Are Conserved in the Genome of the Wine Yeast Hanseniaspora uvarum, and Pyruvate Kinase Limits Its Capacity for Alcoholic Fermentation.

    Science.gov (United States)

    Langenberg, Anne-Kathrin; Bink, Frauke J; Wolff, Lena; Walter, Stefan; von Wallbrunn, Christian; Grossmann, Manfred; Heinisch, Jürgen J; Schmitz, Hans-Peter

    2017-11-15

    Hanseniaspora uvarum (anamorph Kloeckera apiculata ) is a predominant yeast on wine grapes and other fruits and has a strong influence on wine quality, even when Saccharomyces cerevisiae starter cultures are employed. In this work, we sequenced and annotated approximately 93% of the H. uvarum genome. Southern and synteny analyses were employed to construct a map of the seven chromosomes present in a type strain. Comparative determinations of specific enzyme activities within the fermentative pathway in H. uvarum and S. cerevisiae indicated that the reduced capacity of the former yeast for ethanol production is caused primarily by an ∼10-fold-lower activity of the key glycolytic enzyme pyruvate kinase. The heterologous expression of the encoding gene, H. uvarum PYK1 ( HuPYK1 ), and two genes encoding the phosphofructokinase subunits, HuPFK1 and HuPFK2 , in the respective deletion mutants of S. cerevisiae confirmed their functional homology. IMPORTANCE Hanseniaspora uvarum is a predominant yeast species on grapes and other fruits. It contributes significantly to the production of desired as well as unfavorable aroma compounds and thus determines the quality of the final product, especially wine. Despite this obvious importance, knowledge on its genetics is scarce. As a basis for targeted metabolic modifications, here we provide the results of a genomic sequencing approach, including the annotation of 3,010 protein-encoding genes, e.g., those encoding the entire sugar fermentation pathway, key components of stress response signaling pathways, and enzymes catalyzing the production of aroma compounds. Comparative analyses suggest that the low fermentative capacity of H. uvarum compared to that of Saccharomyces cerevisiae can be attributed to low pyruvate kinase activity. The data reported here are expected to aid in establishing H. uvarum as a non- Saccharomyces yeast in starter cultures for wine and cider fermentations. Copyright © 2017 American Society for

  17. Fermentation of Xylose Causes Inefficient Metabolic State Due to Carbon/Energy Starvation and Reduced Glycolytic Flux in Recombinant Industrial Saccharomyces cerevisiae

    Science.gov (United States)

    Matsushika, Akinori; Nagashima, Atsushi; Goshima, Tetsuya; Hoshino, Tamotsu

    2013-01-01

    In the present study, comprehensive, quantitative metabolome analysis was carried out on the recombinant glucose/xylose-cofermenting S. cerevisiae strain MA-R4 during fermentation with different carbon sources, including glucose, xylose, or glucose/xylose mixtures. Capillary electrophoresis time-of-flight mass spectrometry was used to determine the intracellular pools of metabolites from the central carbon pathways, energy metabolism pathways, and the levels of twenty amino acids. When xylose instead of glucose was metabolized by MA-R4, glycolytic metabolites including 3- phosphoglycerate, 2- phosphoglycerate, phosphoenolpyruvate, and pyruvate were dramatically reduced, while conversely, most pentose phosphate pathway metabolites such as sedoheptulose 7- phosphate and ribulose 5-phosphate were greatly increased. These results suggest that the low metabolic activity of glycolysis and the pool of pentose phosphate pathway intermediates are potential limiting factors in xylose utilization. It was further demonstrated that during xylose fermentation, about half of the twenty amino acids declined, and the adenylate/guanylate energy charge was impacted due to markedly decreased adenosine triphosphate/adenosine monophosphate and guanosine triphosphate/guanosine monophosphate ratios, implying that the fermentation of xylose leads to an inefficient metabolic state where the biosynthetic capabilities and energy balance are severely impaired. In addition, fermentation with xylose alone drastically increased the level of citrate in the tricarboxylic acid cycle and increased the aromatic amino acids tryptophan and tyrosine, strongly supporting the view that carbon starvation was induced. Interestingly, fermentation with xylose alone also increased the synthesis of the polyamine spermidine and its precursor S-adenosylmethionine. Thus, differences in carbon substrates, including glucose and xylose in the fermentation medium, strongly influenced the dynamic metabolism of MA-R4

  18. Different TP53 mutants in p53 overexpressed epithelial ovarian carcinoma can be associated both with altered and unaltered glycolytic and apoptotic profiles.

    Science.gov (United States)

    Antoun, Stephanie; Atallah, David; Tahtouh, Roula; Alaaeddine, Nada; Moubarak, Malak; Khaddage, Abir; Ayoub, Eliane Nasr; Chahine, George; Hilal, George

    2018-01-01

    p53 is a tumor suppressor and key regulator of glycolysis in cancer cells, however highly mutated in tumors. In ovarian cancer, studies concerning p53 mutations focus on the DNA binding domain since the majority of hotspot mutations affects this region. Yet, mutations in other regions such as the proline rich domain may also affect the protein's expression and activity. The aim of this study is to investigate the effect of various positions of mutations in TP53 gene on glycolysis, apoptosis and transcription of p53 target genes. Mutations frequency and their effect on p53 expression were assessed by PCR-SSCP, sequencing and immunohistochemistry on 30 ovarian cancer biopsies. Six tumors were cultured, as well as SK-OV-3, OVCAR-3 and Igrov-1. SK-OV-3 cells were transfected with 2 TP53 mutants. p53 transcriptional activity was assayed by qPCR, apoptosis by flow cytometry and glycolysis by glucose and lactate measurements, with quantification of glycolytic enzymes expression. Our results showed a high frequency of the P72R mutant, associated with p53 overexpression in the ovarian biopsies. However, P72R mutant cells showed similar apoptosis and glycolysis as WT cells. DNA binding domain mutations decreased the transcriptional activity of the protein and increased glucose consumption and lactate production. Despite the overexpression of the P72R mutated protein in the biopsies, it showed a similar apoptotic activity and glucose regulation ability as WT p53. Knowing that p53 expression status is used for chemotherapeutic approaches and prognosis in ovarian cancer, the results obtained highlight the importance of locating TP53 mutations.

  19. Increased OXPHOS activity precedes rise in glycolytic rate in H-RasV12/E1A transformed fibroblasts that develop a Warburg phenotype

    Directory of Open Access Journals (Sweden)

    Pluk Helma

    2009-07-01

    Full Text Available Abstract Background The Warburg phenotype in cancer cells has been long recognized, but there is still limited insight in the consecutive metabolic alterations that characterize its establishment. We obtained better understanding of the coupling between metabolism and malignant transformation by studying mouse embryonic fibroblast-derived cells with loss-of-senescence or H-RasV12/E1A-transformed phenotypes at different stages of oncogenic progression. Results Spontaneous immortalization or induction of senescence-bypass had only marginal effects on metabolic profiles and viability. In contrast, H-RasV12/E1A transformation initially caused a steep increase in oxygen consumption and superoxide production, accompanied by massive cell death. During prolonged culture in vitro, cell growth rate increased gradually, along with tumor forming potential in in vitro anchorage-independent growth assays and in vivo tumor formation assays in immuno-deficient mice. Notably, glucose-to-lactic acid flux increased with passage number, while cellular oxygen consumption decreased. This conversion in metabolic properties was associated with a change in mitochondrial NAD+/NADH redox, indicative of decreased mitochondrial tricarboxic acid cycle and OXPHOS activity. Conclusion The high rate of oxidative metabolism in newly transformed cells is in marked contrast with the high glycolytic rate in cells in the later tumor stage. In our experimental system, with cells growing under ambient oxygen conditions in nutrient-rich media, the shift towards this Warburg phenotype occurred as a step-wise adaptation process associated with augmented tumorigenic capacity and improved survival characteristics of the transformed cells. We hypothesize that early-transformed cells, which potentially serve as founders for new tumor masses may escape therapies aimed at metabolic inhibition of tumors with a fully developed Warburg phenotype.

  20. Proteomic changes in renal cancer and co-ordinate demonstration of both the glycolytic and mitochondrial aspects of the Warburg effect.

    Science.gov (United States)

    Unwin, Richard D; Craven, Rachel A; Harnden, Patricia; Hanrahan, Sarah; Totty, Nick; Knowles, Margaret; Eardley, Ian; Selby, Peter J; Banks, Rosamonde E

    2003-08-01

    Renal cell carcinoma (RCC) is the tenth most common cancer although the incidence is increasing. The main clinical problems stem from the relatively late presentation of many patients due to the often asymptomatic nature of the illness, and the relative insensitivity of metastatic disease to conventional chemotherapy and radiotherapy. Despite increasing knowledge of some of the genetic changes underlying sporadic renal cancer such as those involving the Von Hippel Lindau (VHL) gene, many of the underlying pathophysiological changes are ill-defined and there remains a need for the identification of disease markers for use in diagnosis and prognosis or as potential therapeutic targets. This study has used a proteomic approach, based on two-dimensional gel electrophoresis and mass spectrometry, to compare the protein profiles of conventional RCC tissue with patient-matched normal kidney cortex. Sequencing of 32 protein spots with significantly increased expression in RCC samples (>/= 4/6 patients) and 41 proteins whose levels decreased (6/6 patients) confirmed several previously known RCC-associated changes such as increases in Mn-superoxide dismutase, lactate dehydrogenase-A, aldolase A and C, pyruvate kinase M2, and thymidine phosphorylase. Additionally, several previously unknown changes were identified, including increased expression of three members of the annexin family and increased levels of the actin depolymerisation factor cofilin. The Warburg effect was also demonstrated with the identification of increases in proteins involved in the majority of steps in the glycolytic pathway and decreases in the gluconeogenic reactions, together with a parallel decrease in several mitochondrial enzymes. A number of the alterations seen were further confirmed in additional samples by immunohistochemistry, Western blotting, and laser capture microdissection.

  1. [Marfan syndrome: clinical and pathomorphological restructurings after surgical treatment of aortic aneurysm].

    Science.gov (United States)

    Zhuraev, R K; Zerbino, D D

    2014-01-01

    The authors examined the state of patients suffering from Marfan syndrome (MS) who endured operation for ascending aorta aneurysm with replacement of the ascending aorta and aortic valve (Bentall operation), studying alterations of the skeleton, face, heart and eyes, as well as pathomorphological restructurings in the aortic wall. The study was carried out 7.0 ± 4.2 years after the operation. We examined a total of 39 patients with MS - 27 (69.2%) men and 12 (30.8%) women aged from 22 to 70 years old (average age - 42.1 ± 13.4 years). All patients were operated on for dissecting aneurysm of the ascending aorta accompanied by a considerable degree of aortic valve insufficiency or aortic ostium stenosis. The mean diameter of the aorta at the level of the sinuses of Valsalva amounted to 7.0 ± 1.3 cm (minimal - 5.0 cm, maximal - 12.0 cm), the Z-score prior to operation was 12.7 ± 6.5. The time form making the diagnosis of MS to surgical intervention for aortic aneurysm amounted to 9.6 ± 5.9 years. The condition after operative treatment in all patients was satisfactory, with the haemodynamic indices stable: systolic AP - 133.5 ± 19.1 mm Hg, diastolic AP 85.1 ± 12.9 mm Hg, heart rate 74.8 ± 7.2 bpm. The average systemic score for the symptoms and tests of MS patients amounted to 8.2 ± 3.3 points. Pathohistological alterations of the aorta in patients with Marfan syndrome consisted in pronounced restructuring of the wall with deep irreversible alternative changes. The pathological process extended in the middle aortic layer all alone the length, but not only in the portions of rupture and dissection. The main pathomorphological signs in MS were as follows: focal accumulations of mucoid substances, dystrophic alterations of smooth-muscle cells, ribbon-like anuclear zones, formation of cystlike cavities, alterations of elastic fibres - fragmentation, hyperelastosis, multiplication, thinning and straightening, zones of elastolysis.

  2. Is debt restructuring needed to make the Stability and Growth Pact (more credible?

    Directory of Open Access Journals (Sweden)

    Jens Körner

    2011-01-01

    Full Text Available The emergence of the so-called PIIGS crisis which in 2009 became acute due to strongly diverging risk premiums, marked the beginning of a new phase for the European Monetary Union. Whilst the run-up to EMU had been characterized by an encouraging convergence of macroeconomic fundamentals of its member countries, it is now facing a serious threat in particular due to excessive levels of public debt. In 1997, the Stability and Growth Pact introduced a mechanism designed to prevent excessive public debt of the type currently observed; the fact of the matter, however, seems to be that levels of public debt has continuously grown from one economic cycle to the next. But the SGP apparently not only failed to fulfil its aim to keep the deficit and the debt level within its limits but also suffered from a severe loss of credibility; unless some profound action is taken it may further diminish, severely hampering the loose structure of the European Monetary Union. In order to regain some credibility, mitigate financial market’s concerns and, hence, lower borrowing cost, a consolidation path is needed to returns to acceptable levels of debt in the foreseeable future. This process has already started and measures have been taken by several eurozone countries to speed up fiscal consolidation. The aim of the paper is to analyze whether or not the group of the PIIGS countries are likely to return to debt levels in accordance with the SGP criteria or if it might be necessary to undergo a process of debt restructuring or default. By analysing different scenarios where nominal interest rates on debt (r and nominal growth rates (n as well as gaps thereof (r-n, herein called the automatic debt dynamics, are varied, this paper comes to the conclusion, that debt restructuring or default is a likely outcome for some of the PIIGS; arithmetics is in particular playing against Greece. As disillusioning and disappointing this outcome might be for some observers, it

  3. Electric Industry Restructuring in Ohio: Residential and Low Income Customer Impacts; TOPICAL

    International Nuclear Information System (INIS)

    Eisenberg, J

    2001-01-01

    This report analyzes the electric utilities in Ohio in order to determine how they are situated for the coming of competition. It begins with the status of the utilities as of 1995, the last year for which detailed data were available, and determines the detailed underlying cost structure behind the rates charged to customers. The study then develops a number of restructuring scenarios to be analyzed. These scenarios cover different approaches to dividing stranded asset costs between customers and stockholders, and between different groups of customers. They also cover wholesale versus retail competition, different regulatory structures for those services still under regulation, and new approaches to stranded asset costs such as securitization--the use of special bonds to reduce costs. Throughout the report the special emphasis is on the impact of restructuring on low-income residential customers. Low-income customers are the most vulnerable to changes in the regulatory structure with the fewest alternative options. The report finds that there are a great deal of above-market cost, potentially stranded assets in Ohio--approximately$8.75 billion in 1995. The annual above-market costs total over$3 billion, of which about 2/3 is recovery of capital related costs and 1/3 is recovery of energy related costs. The distribution of stranded assets in Ohio is very uneven. Some utilities such as Cleveland Electric and Ohio Edison have very high levels of above-market costs. In contrast, Ohio Power has, under some estimates, costs which are actually below market costs. The study looks separately at the near-term or transition period (approximately the next seven to ten years) and the longer term competitive market period. During the transition period the costs of stranded assets are being collected from customers while competitive markets are being developed. In the longer term market period it is assumed that all of the stranded asset costs have been collected and that the

  4. Restructuring of the Aquatic Bacterial Community by Hydric Dynamics Associated with Superstorm Sandy.

    Science.gov (United States)

    Ulrich, Nikea; Rosenberger, Abigail; Brislawn, Colin; Wright, Justin; Kessler, Collin; Toole, David; Solomon, Caroline; Strutt, Steven; McClure, Erin; Lamendella, Regina

    2016-06-15

    Bacterial community composition and longitudinal fluctuations were monitored in a riverine system during and after Superstorm Sandy to better characterize inter- and intracommunity responses associated with the disturbance associated with a 100-year storm event. High-throughput sequencing of the 16S rRNA gene was used to assess microbial community structure within water samples from Muddy Creek Run, a second-order stream in Huntingdon, PA, at 12 different time points during the storm event (29 October to 3 November 2012) and under seasonally matched baseline conditions. High-throughput sequencing of the 16S rRNA gene was used to track changes in bacterial community structure and divergence during and after Superstorm Sandy. Bacterial community dynamics were correlated to measured physicochemical parameters and fecal indicator bacteria (FIB) concentrations. Bioinformatics analyses of 2.1 million 16S rRNA gene sequences revealed a significant increase in bacterial diversity in samples taken during peak discharge of the storm. Beta-diversity analyses revealed longitudinal shifts in the bacterial community structure. Successional changes were observed, in which Betaproteobacteria and Gammaproteobacteria decreased in 16S rRNA gene relative abundance, while the relative abundance of members of the Firmicutes increased. Furthermore, 16S rRNA gene sequences matching pathogenic bacteria, including strains of Legionella, Campylobacter, Arcobacter, and Helicobacter, as well as bacteria of fecal origin (e.g., Bacteroides), exhibited an increase in abundance after peak discharge of the storm. This study revealed a significant restructuring of in-stream bacterial community structure associated with hydric dynamics of a storm event. In order to better understand the microbial risks associated with freshwater environments during a storm event, a more comprehensive understanding of the variations in aquatic bacterial diversity is warranted. This study investigated the bacterial

  5. Changing winemaking industry, recomposition of labour relations, restructuration of the territory. The case of Barranca, Argentina (1995-2010

    Directory of Open Access Journals (Sweden)

    Lorena Poblete

    2014-03-01

    Full Text Available Since the 90s, the winemaking process undergoes an accelerated restructuration. The production model focused on the low quality wines for the domestic market is crossed by a new model of production of fine wines destined for international trade. As a result of this new model, the territory was reorganized both as regards their use as its appropriation. The goal of this paper is to study the transformation of Mendoza winemaking industry through analysis the way that the territory was restructured. If changes in the territory are a consequence of the new production model, there are also determinants for their evolution over time. It is precisely this second aspect that we wish to emphasize here.

  6. REGIONAL PROBLEMS OF THE ASSESSMENT AND MANAGEMENT OF THE COST OF PROPERTY AND RESTRUCTURING OF THE INDUSTRIAL ENTERPRISES IN SYSTEM OF STRATEGIC MANAGEMENT

    Directory of Open Access Journals (Sweden)

    Anna R. Plotkina

    2015-01-01

    Full Text Available In this article the author reveals, provesand systematizes regional problems ofan assessment and management of thecost of property and restructuring of theindustrial enterprises in system of strategic management.

  7. An empirical inquiry into the objective assessment and the top management perception of the need for organisational restructuring in large Croatian enterprises

    Directory of Open Access Journals (Sweden)

    Nikša Alfirević

    2000-01-01

    Full Text Available On the basis of the extensive empirical research, this paper examines the objective need for organisational restructuring in large Croatian enterprises versus the managerial perceptions of the same issue. Building upon the analysis of the economic performance of the Croatian large enterprise sector and the assessment of the organisational design quality in large Croatian enterprises, the urgency for further organisational restructuring is established. On the other hand, the major restructuring motives at the top management level are also examined, leading to the conclusion that both the perceived quality of the organisational design, as well as the perceived gap between the enterprise competitive ability and the threats from the business environment at the top management level do not support the further restructuring of the Croatian large enterprise sector.

  8. Network reconstruction using nonparametric additive ODE models.

    Science.gov (United States)

    Henderson, James; Michailidis, George

    2014-01-01

    Network representations of biological systems are widespread and reconstructing unknown networks from data is a focal problem for computational biologists. For example, the series of biochemical reactions in a metabolic pathway can be represented as a network, with nodes corresponding to metabolites and edges linking reactants to products. In a different context, regulatory relationships among genes are commonly represented as directed networks with edges pointing from influential genes to their targets. Reconstructing such networks from data is a challenging problem receiving much attention in the literature. There is a particular need for approaches tailored to time-series data and not reliant on direct intervention experiments, as the former are often more readily available. In this paper, we introduce an approach to reconstructing directed networks based on dynamic systems models. Our approach generalizes commonly used ODE models based on linear or nonlinear dynamics by extending the functional class for the functions involved from parametric to nonparametric models. Concomitantly we limit the complexity by imposing an additive structure on the estimated slope functions. Thus the submodel associated with each node is a sum of univariate functions. These univariate component functions form the basis for a novel coupling metric that we define in order to quantify the strength of proposed relationships and hence rank potential edges. We show the utility of the method by reconstructing networks using simulated data from computational models for the glycolytic pathway of Lactocaccus Lactis and a gene network regulating the pluripotency of mouse embryonic stem cells. For purposes of comparison, we also assess reconstruction performance using gene networks from the DREAM challenges. We compare our method to those that similarly rely on dynamic systems models and use the results to attempt to disentangle the distinct roles of linearity, sparsity, and derivative

  9. An Evaluation of the 1989 Restructuring of the Navy Medical Department from the Perspective of Financial Managers

    Science.gov (United States)

    1991-12-01

    West Publishing Company, 1989. 14. Chung, Kae H., Management: Critical Success Factors, Boston: Allyn and Bacon Inc., 1987. 15. Beckhard , Richard and...THE 1989 RESTRUCTURING ........ .. 69 B. CHANGE MODELS ...... ................. 70 1. The Griffin and Curzon Models . ....... 70 2. The Beckhard Model...enhances or constrains what an organization is able to accomplish. [Ref. 7 :p. 46] Beckhard and Harris, drawing upon the work of Chandler, Galbraith and

  10. The restructuring of institutional long-term care in St. John's: impact of supply-induced demand on planning.

    Science.gov (United States)

    Wilson, Robert C; McDonald, Jacqueline; Barrett, Brendan; Parfrey, Patrick S

    2011-01-01

    Restructuring of institutional long-term care was undertaken using predictions of future bed need with assumptions made on incidence rates of clients defined by type of disability, survival, and demographic changes. Recent substantial increase in the population rate of clients seeking placement across all degrees of disability, coincident with new facilities for those with modest disability, occurred. Consequently, more appropriate housing and supervised care beds, and more limited downsizing of nursing homes will be required.

  11. "Job Quality, Labor Market Segmentation, and Earning Inequality: Effects of Economic Restructuring in the 1980s by Race and Gender"

    OpenAIRE

    Maury B. Gittleman; David R. Howell

    1992-01-01

    The authors examine the effects of employment restructuring in the 1980s on white, black, and Hispanic men and women within a labor market segmentation framework. Cluster analysis is used to determine whether jobs can be grouped into a small number of relatively homogeneous clusters on the basis of differences in job quality. With data centered on 1979, 621 occupation/ industry cells covering 94% of the workforce are analyzed with 17 measures of job quality, ranging from earnings and benefits...

  12. Surgical Pathology Resident Rotation Restructuring at a Tertiary Care Academic Center

    Directory of Open Access Journals (Sweden)

    Chelsea R. Mehr MD

    2017-11-01

    Full Text Available Changes in the field of pathology and resident education necessitate ongoing evaluation of residency training. Evolutionary change is particularly important for surgical pathology rotations, which form the core of anatomic pathology training programs. In the past, we organized this rotation based on subjective insight. When faced with the recent need to restructure the rotation, we strove for a more evidence-based process. Our approach involved 2 primary sources of data. We quantified the number of cases and blocks submitted per case type to estimate workload and surveyed residents about the time required to gross specimens in all organ systems. A multidisciplinary committee including faculty, residents, and staff evaluated the results and used the data to model how various changes to the rotation would affect resident workload, turnaround time, and other variables. Finally, we identified rotation structures that equally distributed work and created a point-based system that capped grossing time for residents of different experience. Following implementation, we retrospectively compared turnaround time and duty hour violations before and after these changes and surveyed residents about their experiences with both systems. We evaluated the accuracy of the point-based system by examining grossing times and comparing them to the assigned point values. We found overall improvement in the rotation following the implementation. As there is essentially no literature on the subject of surgical pathology rotation organization, we hope that our experience will provide a road map to improve pathology resident education at other institutions.

  13. The Economic Ascent of China and the Potential for Restructuring the Capitalist World-Economy

    Directory of Open Access Journals (Sweden)

    Paul S. Ciccantell

    2015-08-01

    Full Text Available The economic ascent of China in the past two decades is the most dramatic change in the capitalist world-economy of this period. Analyses focus on changes in government control of the economy, the availability of low cost workers for export production, the historical characteristics of Chinese economy and society, and the role of the Chinese government as a developmental state. All highlight key parts of China’s economic ascent, but none addresses what we argue will be the critical component of future sustained economic ascent, if it is to take place in China: the role of raw materials and transport industries as generative sectors. These generative sectors in the most successful historical cases articulate domestic economic development with the creation ofnew systems of international economic and political relations, ultimately restructuring the capitalist world-economy in support of a nation’s ascent to core status and its ability to challenge the existing hegemon and other ascendant economies for hegemony. China is following the Japanese model of coastal greenfield heavy industrialization as state policies focus on deepening industrialization in steel, shipbuilding, and other heavy industries. However, following the models of earlier ascendant economies does not guarantee success. In this paper, we analyze the efforts underway in China to use steel, coal and other linked industries as driving forces for sustained economic ascent, and the potential consequences of these efforts for China and for the world economy.

  14. Self-Healing Thermal Annealing: Surface Morphological Restructuring Control of GaN Nanorods

    Energy Technology Data Exchange (ETDEWEB)

    Conroy, Michele; Li, Haoning; Zubialevich, Vitaly Z.; Kusch, Gunnar; Schmidt, Michael; Collins, Timothy; Glynn, Colm; Martin, Robert W.; O’Dwyer, Colm; Morris, Michael D.; Holmes, Justin D.; Parbrook, Peter J.

    2016-12-07

    With advances in nanolithography and dry etching, top-down methods of nanostructuring have become a widely used tool for improving the efficiency of optoelectronics. These nano dimensions can offer various benefits to the device performance in terms of light extraction and efficiency, but often at the expense of emission color quality. Broadening of the target emission peak and unwanted yellow luminescence are characteristic defect-related effects due to the ion beam etching damage, particularly for III–N based materials. In this article we focus on GaN based nanorods, showing that through thermal annealing the surface roughness and deformities of the crystal structure can be “self-healed”. Correlative electron microscopy and atomic force microscopy show the change from spherical nanorods to faceted hexagonal structures, revealing the temperature-dependent surface morphology faceting evolution. The faceted nanorods were shown to be strain- and defect-free by cathodoluminescence hyperspectral imaging, micro-Raman, and transmission electron microscopy (TEM). In-situ TEM thermal annealing experiments allowed for real time observation of dislocation movements and surface restructuring observed in ex-situ annealing TEM sampling. This thermal annealing investigation gives new insight into the redistribution path of GaN material and dislocation movement post growth, allowing for improved understanding and in turn advances in optoelectronic device processing of compound semiconductors.

  15. Restructuring terminated. Rotor technology is lookingt for an investor.; Restrukturierung abgeschlossen. Rotortechnik sucht Investor

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2006-07-01

    The NOI Group was declared insolvent in July 2004. Attorney-at-law Rolf Rombach of Erfurt was appointed as the administrator for NOI-Rotortechnik GmbH, Nordhausen. The other former production facilities of the NOI Group in Germany, Scotland and Spain are no longer active. More than 1,000 rotor blades have been mass-produced in Nordhausen since the company was founded in 1999. The site covers an area of 60,000 m{sup 2}, including around 8,500 m{sup 2} of production facilities. More than Euro 10 million in total has been invested in the production facilities. The management - consisting of Niels Ludwig, Juergen Simon and Carsten Zaremba - is now taking the business successfully forward together with the administrator. With its 80-strong workforce, Rotortechnik produces top-quality rotor blades in the multi-megawatt class for German and international customers. During the past 20 months all the work processes have been critically examined, reorganised and constantly optimised. Following complete restructuring, Rotortechnik GmbH in Nordhausen is now ready to make a high-level comeback together with an investor. (orig.)

  16. Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.

    Science.gov (United States)

    Ahmad, Rizwan; Rah, Bilal; Bastola, Dhundy; Dhawan, Punita; Singh, Amar B

    2017-07-11

    Obesity increases susceptibility to multiple organ disorders, however, underlying mechanisms remain unclear. The subclinical inflammation assisted by obesity-induced gut permeability may underlie obesity-associated co-morbidities. Despite eminent clinical significance of the obesity led gut barrier abnormalities, its precise molecular regulation remains unclear. It is also unknown whether barrier deregulations, similar to the gut, characterize other vital organs in obese individuals. The claudin family of proteins is integral to the tight junction (TJ), the apical cell-cell adhesion and a key regulator of the epithelial barrier. Using comprehensive physiological and biochemical analysis of intestinal and renal tissues from high-fat diet fed mice, critical for maintaining metabolic homeostasis, this study demonstrates that profound TJ-restructuring by organ and tissue-specific claudin switching characterize obese organs. Protein expression and cellular distribution were examined. In-silico analysis further highlighted potential association of select claudins, modulated by the obesity, with signaling and metabolic pathways of pathological significance. In vitro studies using Leptin or DCA-treatment suggested causal significance of obesity-induced changes in tissue microenvironment in regulating barrier deregulations in tissue-specific manner. Overall, current findings advances our understanding of the molecular undertakings of obesity associated changes that help predispose to specific diseases and also identifies novel windows of preventive and/or therapeutic interventions.

  17. The Simulation Study of a Restructured Residential Low-Voltage Distribution System Microgrid

    Directory of Open Access Journals (Sweden)

    L. Ramesh

    2016-12-01

    Full Text Available A primary and necessary focus in creating a greener environment is the conversion of existing power-generation sources to renewable power sources in the near future. Another important focus is to develop sustainable household power generation to a low-voltage electricity grid with a power purchase and selling facility. To help with achieving the above vision, the objective of this work is to critically analyze the existing low-voltage distribution system and make suggestions for restructuring it to the low-voltage interconnected microgrid (MG. The test was carried out in the Tamil Nadu Electricity Board (TNEB 100kVA transformer feeder which was connected to supply around 100 houses with electricity. The performance analysis of the proposed system was examined through different case studies, represented as a normal operating condition of the existing distribution system and a reconstructed and interconnected MG to the TNEB grid. The project was designed and analyzed using PSCAD software. The results discussed in the project are helpful in examining the effects of multiple distributed energy resources on distributed generation. In future, knowledge of these effects may be helpful for rural area electrification.

  18. Restructuring: new relationships between the oil companies and the upstream oil firms

    International Nuclear Information System (INIS)

    Barreau, S.

    2001-11-01

    Since the 1986 oil shock, international oil companies have focused on their base competencies, concentrating on activities viewed as their core businesses and steadily increasing the number of tasks to be subcontracted to the upstream oil and gas service sector. The upstream oil and gas service companies had to be restructured to face this new challenge. The strategies they launched at the end of the 80's were varied. Some firms became largely integrated (Schlumberger, Baker Hughes, Halliburton) whereas other firms chose to broaden their range of services. However generally, they opted for external investment which led to an important wave of mergers and acquisitions. The first part characterizes the upstream oil and gas sector by introducing the main oil and gas service firms and their recent strategic evolution. This concludes with both an economic valuation and a typology of attempted growth strategies. To illustrate this, a matrix has been created to characterise the dynamic paths of the oil and gas service firms. The purpose of the second part is to consider the economic theories related to industrial strategies. The strategies of innovation, market protection, vertical integration and diversification have been studied to illustrate the main conclusion which is that the aim of all these strategies was to change the relationships between the oil companies and the upstream oil and gas service firms. (author)

  19. Reaction-Driven Restructuring of Rh-Pd and Pt-Pd Core-Shell Nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Tao, Feng; Grass, Michael E.; Zhang, Yawen; Butcher, Derek R.; Renzas, James R.; Liu, Zhi; Chung, Jen Y.; Mun, Bongjin S.; Salmeron, Miquel; Somorjai, Gabor A.

    2009-06-17

    The structure and composition of core-shell Rh{sub 0.5}Pd{sub 0.5} and Pt{sub 0.5}Pd{sub 0.5} nanoparticle catalysts were studied in situ, during oxidizing, reducing, and catalytic reactions involving NO, O{sub 2}, CO, and H{sub 2} using X-ray photoelectron spectroscopy in the Torr pressure range. The Rh{sub 0.5}Pd{sub 0.5} nanoparticles undergo dramatic and reversible changes in composition and chemical state in response to oxidizing or reducing conditions. Under oxidizing conditions the Rh atoms segregate to the shell region while in reducing atmospheres the Pd atoms diffuse to the shell region. In contrast no significant segregation of Pd or Pt atoms was found in Pt{sub 0.5}Pd{sub 0.5} nanoparticles. The distinct behavior in restructuring and chemical response of Rh{sub 0.5}Pd{sub 0.5} and Pt{sub 0.5}Pd{sub 0.5} nanoparticle catalysts under the same reaction conditions illustrates the flexibility and tunability of the structure of bimetallic nanoparticle catalysts during catalytic reactions.

  20. Electrification of the transportation sector: Is there a need for restructuring the automotive parts suppliers?

    Science.gov (United States)

    Bekaslan, A. Ebru

    The future of the transportation sector has significant implications for the mitigation of climate change through reduced GHG emissions as well as achieving energy efficiency and energy independence. Battery-powered, plug-in, and hybrid electric vehicles are widely seen as the greatest source of a solution. This thesis analyzes the historical development and growth of the automotive industry as well as the restructuring toward the next generation technologies in comparison with the U.S. and China to shed light on the question of how a developing country can structure its strategies to be able to upgrade and be competitive over time. Conventional business models can become obsolete. Companies will find it difficult to maintain their market position unless they gain new perspectives on the outlook of the industry as a whole, and take into account the successful business models of tomorrow. The potential for further growth of the Turkish automotive supplier base particularly in the next generation of automotive technologies is therefore highly dependent on policies and strategies at the national level as much as its dependence on the global strategies of the automotive industry's major players. In this sense, the key questions that motivate this study are whether there are foreseeable technological changes and product-segmentation strategies that could significantly enhance the competitiveness of local Turkish suppliers of automotive components. The thesis builds on the theory of innovative enterprise and evolutionary agglomeration to examine the competitive potential of the Konya Auto Part Suppliers' Cluster in Turkey.