WorldWideScience

Sample records for glycolytic network restructuring

  1. Neural Network Based Load Frequency Control for Restructuring ...

    African Journals Online (AJOL)

    Neural Network Based Load Frequency Control for Restructuring Power Industry. ... an artificial neural network (ANN) application of load frequency control (LFC) of a Multi-Area power system by using a neural network controller is presented.

  2. Coarsening by network restructuring in model nanoporous gold

    International Nuclear Information System (INIS)

    Kolluri, Kedarnath; Demkowicz, Michael J.

    2011-01-01

    Using atomistic modeling, we show that restructuring of the network of interconnected ligaments causes coarsening in a model of nanoporous gold. The restructuring arises from the collapse of some ligaments onto neighboring ones and is enabled by localized plasticity at ligaments and nodes. This mechanism may explain the occurrence of enclosed voids and reduction in volume in nanoporous metals during their synthesis. An expression is developed for the critical ligament radius below which coarsening by network restructuring may occur spontaneously, setting a lower limit to the ligament dimensions of nanofoams.

  3. Capillary network in slow and fast muscles and in oxidative and glycolytic muscle fibres

    Czech Academy of Sciences Publication Activity Database

    Čebašek, V.; Kubínová, Lucie; Ribarič, S.; Eržen, I.

    2005-01-01

    Roč. 24, March (2005), s. 51-58 ISSN 1580-3139 Grant - others:SI-CZ(CZ) KONTAKT 19/2005 Institutional research plan: CEZ:AV0Z50110509 Keywords : capillaries * skeletal muscle fibre s-oxidative and glycolytic * stereology Subject RIV: EA - Cell Biology

  4. Development and Restructuring of Regional Production/Distribution Networks in East Asia

    OpenAIRE

    Mitsuyo ANDO

    2013-01-01

    This paper attempts to investigate the features of development and restructuring patterns of production/distribution networks in East Asia, mainly in machinery sectors, using international trade data at the most disaggregated level, to discuss their resilient nature, and to provide policy implications for the regional production networks and the economic development in the region. Although the negative impacts of the Global Financial Crisis (GFC) in 2008 were initially transmitted through the...

  5. neural network based load frequency control for restructuring power

    African Journals Online (AJOL)

    2012-03-01

    Mar 1, 2012 ... the system in the back propagation chain used in controller training. For this application, .... The partial derivative of E with respect to ele- ments of Γ, for example W, ... Ki = any non-negative value. Figure 7: Neural Network ...

  6. Electric restructuring and consumer choice: lessons from other network industries

    International Nuclear Information System (INIS)

    Crandall, R. W.

    1999-01-01

    The advantages of the U.S. model of private markets with limited regulation as the best alternative for delivering goods and services to consumers are discussed by citing examples from deregulated industries such as transportation, primary energy and financial markets. In all these cases deregulation has been extraordinarily successful. Experiences from these industries are examined in an effort to extract lessons that might be useful in predicting the likely evolution of competition in the electricity and telecommunications industries. A warning is sounded that deregulating these industries without opening access to the infrastructure (which is owned by carriers) could create major problems of natural-monopoly exploitation by the incumbents that would negate any productive and allocative efficiency gains conferred by deregulation. One obvious choice for liberalizing a network industry with natural-monopoly infrastructure is simply to separate the infrastructure from the delivery of the service as was done with railroads in the United Kingdom. A similar, but less far-reaching example might be the solution devised for natural gas pipelines in the U.S. where pipeline owners opened their infrastructure to competitors, albeit at regulated rates. In the electricity industry, separating power generation from transmission and distribution appears to be fairly simple, provided access to transmission and distribution network is granted. In the telecommunication industry where there is no generation, the natural monopoly may be in the local distribution of traffic to subscribers, hence separation of local distribution from national or regional distribution is the normal way to open up the market to new service providers. Experiences in the U. S., the U. K., Canada and New Zealand in electricity and telecommunications industry deregulation are examined and various pitfalls in current approaches are pointed out. It is the author's contention that announcing a date for the end

  7. Reliability assessment of restructured power systems using reliability network equivalent and pseudo-sequential simulation techniques

    International Nuclear Information System (INIS)

    Ding, Yi; Wang, Peng; Goel, Lalit; Billinton, Roy; Karki, Rajesh

    2007-01-01

    This paper presents a technique to evaluate reliability of a restructured power system with a bilateral market. The proposed technique is based on the combination of the reliability network equivalent and pseudo-sequential simulation approaches. The reliability network equivalent techniques have been implemented in the Monte Carlo simulation procedure to reduce the computational burden of the analysis. Pseudo-sequential simulation has been used to increase the computational efficiency of the non-sequential simulation method and to model the chronological aspects of market trading and system operation. Multi-state Markov models for generation and transmission systems are proposed and implemented in the simulation. A new load shedding scheme is proposed during generation inadequacy and network congestion to minimize the load curtailment. The IEEE reliability test system (RTS) is used to illustrate the technique. (author)

  8. Day-ahead price forecasting in restructured power systems using artificial neural networks

    International Nuclear Information System (INIS)

    Vahidinasab, V.; Jadid, S.; Kazemi, A.

    2008-01-01

    Over the past 15 years most electricity supply companies around the world have been restructured from monopoly utilities to deregulated competitive electricity markets. Market participants in the restructured electricity markets find short-term electricity price forecasting (STPF) crucial in formulating their risk management strategies. They need to know future electricity prices as their profitability depends on them. This research project classifies and compares different techniques of electricity price forecasting in the literature and selects artificial neural networks (ANN) as a suitable method for price forecasting. To perform this task, market knowledge should be used to optimize the selection of input data for an electricity price forecasting tool. Then sensitivity analysis is used in this research to aid in the selection of the optimum inputs of the ANN and fuzzy c-mean (FCM) algorithm is used for daily load pattern clustering. Finally, ANN with a modified Levenberg-Marquardt (LM) learning algorithm are implemented for forecasting prices in Pennsylvania-New Jersey-Maryland (PJM) market. The forecasting results were compared with the previous works and showed that the results are reasonable and accurate. (author)

  9. Adaptive short-term electricity price forecasting using artificial neural networks in the restructured power markets

    International Nuclear Information System (INIS)

    Yamin, H.Y.; Shahidehpour, S.M.; Li, Z.

    2004-01-01

    This paper proposes a comprehensive model for the adaptive short-term electricity price forecasting using Artificial Neural Networks (ANN) in the restructured power markets. The model consists: price simulation, price forecasting, and performance analysis. The factors impacting the electricity price forecasting, including time factors, load factors, reserve factors, and historical price factor are discussed. We adopted ANN and proposed a new definition for the MAPE using the median to study the relationship between these factors and market price as well as the performance of the electricity price forecasting. The reserve factors are included to enhance the performance of the forecasting process. The proposed model handles the price spikes more efficiently due to considering the median instead of the average. The IEEE 118-bus system and California practical system are used to demonstrate the superiority of the proposed model. (author)

  10. What drives Senegalese migration to Europe? The role of economic restructuring, labor demand, and the multiplier effect of networks

    Directory of Open Access Journals (Sweden)

    Pau Baizán

    2016-08-01

    Full Text Available Background: International migration from Sub-Saharan Africa to Europe is poorly understood. Furthermore, existing studies pay insufficient attention to the links between the micro-level factors and political, social, and economic processes in both origin and destination areas. Here we integrate insights from institutional approaches in migration and development research with perspectives that highlight the role of labor market and social capital. Objective: We analyze the contextual and individual-level determinants of migration from Senegal to France, Italy, and Spain since the mid-1970s. We examine the following hypotheses: (1 In Senegal, the deterioration of living conditions and heightened economic insecurity have created the conditions for increasing out-migration propensities. (2 In Europe, labor market restructuring has increased job opportunities in particular places and job niches. (3 In facilitating access of Senegalese migrants to jobs in Europe, social networks have linked these two processes. (4 The conjunction of periods of strong labor demand and the availability of personal networks in Europe creates a boosting effect on the migration probabilities of the Senegalese to Europe. Methods: We use event history models to analyze life course data from the Migrations between Africa and Europe survey (2008. Results: Our empirical results concerning both individual socioeconomic indicators and contextual indicators provide consistent support for the four hypotheses proposed. Conclusions: The initiation and expansion of migration between Senegal and Europe stem from the simultaneous presence of several key factors at origin and destination, including processes of economic restructuring and the mutually reinforcing process of social capital formation and changing labor market conditions. These factors are historically contingent, but they may have a wider relevance in the explanation of migration from developing countries to developed

  11. SACLAY: Restructuring

    International Nuclear Information System (INIS)

    Anon.

    1991-01-01

    In a major restructuring exercise at the French Saclay Laboratory, particle physics, astrophysics and nuclear physics, together with their respective technical support services, have been merged into a single department, to be known as DAPNIA - Department of Astrophysics, Particle Physics, Nuclear Physics and Associated Instrumentation

  12. SACLAY: Restructuring

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1991-12-15

    In a major restructuring exercise at the French Saclay Laboratory, particle physics, astrophysics and nuclear physics, together with their respective technical support services, have been merged into a single department, to be known as DAPNIA - Department of Astrophysics, Particle Physics, Nuclear Physics and Associated Instrumentation.

  13. Restructuring the crystalline cellulose hydrogen bond network enhances its depolymerization rate

    Science.gov (United States)

    Shishir P.S. Chundawat; Giovanni Bellesia; Nirmal Uppugundla; Leonardo da Costa Sousa; Dahai Gao; Albert M. Cheh; Umesh P. Agarwal; Christopher M. Bianchetti; George N. Phillips; Paul Langan; Venkatesh Balan; S. Gnanakaran; Bruce E. Dale

    2011-01-01

    Conversion of lignocellulose to biofuels is partly inefficient due to the deleterious impact of cellulose crystallinity on enzymatic saccharification. We demonstrate how the synergistic activity of cellulases was enhanced by altering the hydrogen bond network within crystalline cellulose fibrils. We provide a molecular-scale explanation of these phenomena through...

  14. Cost restructuring

    International Nuclear Information System (INIS)

    Schmidt, J.A.

    1991-01-01

    This paper reports on the cost restructuring of the petroleum industry. This current decade is likely to be one of the most challenging for the petroleum industry. Though petroleum remains among the world's biggest businesses, news of consolidations, restructuring, and layoffs permeates the oil patch from the Gulf of Mexico to the Arctic Isles. The recessionary economy has accelerated these changes, particularly in the upstream sector. Today, even the best-managed companies are transforming their cost structures, and companies that fail to do likewise probably won't survive as independent companies. Indeed, significant consolidation took place during the 1980s. More consolidations can be expected in this decade for companies that do not adapt to the economic realities of the mature business

  15. Thai restructuring

    International Nuclear Information System (INIS)

    Hayes, David

    1999-01-01

    In preparation for its forthcoming privatization, the Petroleum Authority of Thailand (PTT) has announced its plans for restructuring and these are discussed. PTT is involved in exploration and production of natural gas for both the home and overseas markets, oil refining, oil marketing and sales, and petrochemicals. Statistical data on the company's activities are given. PTT operates about 1600 service stations in Thailand but present thinking is that greater profit will accrue from these sites through sales of 'non-oil products'. (UK)

  16. Management restructuring: toward a leaner organization.

    Science.gov (United States)

    Doerge, J; Hagenow, N

    1995-12-01

    The drive to create integrated delivery networks has given even further momentum to management restructuring. Through centralization of decision making within networks, speed and flexibility are needed to make the difficult modifications required to achieve standardization and general overhead savings. Two hospitals, Carondelet St. Mary's and Carondelet St. Joseph's merged under a single administrative team and one Vice President of Patient Care Services. Outcomes of this restructuring were monetary savings, but also learning how to plan and carry out major management restructuring. The values, the process and the outcomes one year post implementation, and the keys to success in the management restructuring effort are the subjects of this article.

  17. Operational reliability evaluation of restructured power systems with wind power penetration utilizing reliability network equivalent and time-sequential simulation approaches

    DEFF Research Database (Denmark)

    Ding, Yi; Cheng, Lin; Zhang, Yonghong

    2014-01-01

    In the last two decades, the wind power generation has been rapidly and widely developed in many regions and countries for tackling the problems of environmental pollution and sustainability of energy supply. However, the high share of intermittent and fluctuating wind power production has also...... and reserve provides, fast reserve providers and transmission network in restructured power systems. A contingency management schema for real time operation considering its coupling with the day-ahead market is proposed. The time-sequential Monte Carlo simulation is used to model the chronological...

  18. Ontario electricity industry restructuring

    International Nuclear Information System (INIS)

    1999-01-01

    The objective of Ontario's electricity industry restructuring was described as an effort to enhance Ontario's competitiveness. It is believed that restructuring can be accomplished without an increase in electricity rates. In a series of charts, the report provides the timeline of restructuring from the time of the Macdonald report in 1995 to the beginning of open competition in Ontario electricity markets. It oulines the principles underlying the financial restructuring and the financial results of restructuring, including the size of the stranded debt ($ 7.8 billion). It lists the changes that have occurred since October 1998, explains some key factors in valuing the successor companies and profiles the Ontario Electricity Financial Corporation. Restructuring of the industry is expected to have a neutral to positive impact on Ontario's fiscal position. The residual stranded debt of $7.8 billion will be retired through revenues generated by the electricity sector, without recourse to the Provincial Treasury. 9 figs

  19. Restructuring for the 90's...And Beyond. The Era of Smart Homes, Wired Communities, Fast Systems, Global Networks, and Fast Forward Learners in a Borderless World.

    Science.gov (United States)

    Groff, Warren H.

    This paper examines institutional restructuring in higher education for the 1990s and the 21st century and the leadership required for that restructuring. Following an overview and a discussion of selected pertinent demographic, social, economic, technological, and political variables, the paper focuses on the strategic importance of Mid-America…

  20. Lactate: link between glycolytic and oxidative metabolism.

    Science.gov (United States)

    Brooks, George A

    2007-01-01

    Once thought to be the consequence of oxygen lack in contracting skeletal muscle, the glycolytic product lactate is formed and utilised continuously under fully aerobic conditions. 'Cell-cell' and 'intracellular lactate shuttle' concepts describe the roles of lactate in delivery of oxidative and gluconeogenic substrates as well as in cell signalling. Examples of cell-cell shuttles include lactate exchanges (i) between white-glycolytic and red-oxidative fibres within a working muscle bed; (ii) between working skeletal muscle and heart; and (iii) between tissues of net lactate release and gluconeogenesis. Lactate shuttles exist in diverse tissues including in the brain, where a shuttle between astrocytes and neurons is linked to glutamatergic signalling. Because lactate, the product of glycogenolysis and glycolysis, is disposed of by oxidative metabolism, lactate shuttling unites the two major processes of cellular energy transduction. Lactate disposal is mainly through oxidation, especially during exercise when oxidation accounts for 70-75% of removal and gluconeogenesis the remainder. Lactate flux occurs down proton and concentration gradients that are established by the mitochondrial lactate oxidation complex. Marathon running is a power activity requiring high glycolytic and oxidative fluxes; such activities require lactate shuttling. Knowledge of the lactate shuttle is yet to be imparted to the sport.

  1. Global restructuring - A place for ecology?

    NARCIS (Netherlands)

    de Bruijn, Theo; Groenewegen, Peter; Grolin, Jesper

    1997-01-01

    This introductory article summarizes the discussions and results of the Fifth Annual Greening of Industry Conference, Global RestructuringÐA Place for Ecology?, held in Heidelberg, Germany, November 25-27, 1997. The Greening of Industry Network is concerned with the transition of industry towards

  2. SE debt restructuring plan

    International Nuclear Information System (INIS)

    Janoska, J.

    2003-01-01

    Slovenske elektrarne, a.s. (SE) plans to restructure one's own debts in 2003-2005. Debt restructuring plan is following: 2003: Collection of pre-payment on electricity - 60 million Euro (2.5 billion Slovak crowns), consumer unknown. Own promissory notes in total value of 100 million US$ (3.5 billion Slovak crowns) - in process. Sale of claims worth 2.4 billion Slovak crowns (57.21 million Euro) - negotiations in process. 2003/2004: Restructuring of loans payable IV. quarter 2003 and at the beginning of 2004 in value of 200 million Euro (8.3 billion Slovak crowns). Aim of SE is a new credit payable within 7 years, with instalments payable in the last two to three without any state subsidies. 2005: Loans worth 460 million Euro (189 billion Slovak crowns) will still remain. SE want to negotiate them with banks without state support

  3. RESTRUCTURING OF INDUSTRIAL ENTERPRISE

    Directory of Open Access Journals (Sweden)

    L. I. Podderegina

    2009-01-01

    Full Text Available The paper presents main principles for execution of current and strategic restructuring of national enterprises while using experience of countries with developed market economy. The principles contribute to higher efficiency in internal industrial relations at national industrial enterprises.

  4. Corporate Restructuring and Bondholder Wealth

    NARCIS (Netherlands)

    Renneboog, L.D.R.; Szilagyi, P.G.

    2006-01-01

    Abstract: This paper provides an overview of existing research on how corporate restructuring affects the wealth of creditors. Restructuring is defined as any transaction that affects the firm’s underlying capital structure. Thus, it reaches well beyond asset restructuring and includes transactions

  5. ENTERPRISE RESTRUCTURING AIM AND TYPES

    Directory of Open Access Journals (Sweden)

    S. P. Baranenko

    2011-01-01

    Full Text Available Enterprise restructuring is aimed at adapting it to market conditions and improving its competitiveness through selection of most effective model of using material, technical, technological, organizational, commercial, economical, financial, tax-related and other resources with due account of the demand. Restructuring classification signs and types as well as restructuring aims specific for industrial enterprises are provided for.

  6. Corporate Restructuring and Bondholder Wealth

    NARCIS (Netherlands)

    Renneboog, L.D.R.; Szilagyi, P.G.

    2006-01-01

    This paper provides an overview of existing research on how corporate restructuring affects the wealth of creditors.Restructuring is defined as any transaction that affects the firm's underlying capital structure.Thus, it reaches well beyond asset restructuring and includes transactions such as

  7. Modeling diauxic glycolytic oscillations in yeast

    DEFF Research Database (Denmark)

    Hald, Bjørn Olav; Sørensen, Preben Graae

    2010-01-01

    for investigations of central metabolism dynamics of yeast cells. We have previously proposed a model for the open system comprised of the primary fermentative reactions in yeast that quantitatively describes the oscillatory dynamics. However, this model fails to describe the transient behavior of metabolic......Glycolytic oscillations in a stirred suspension of starved yeast cells is an excellent model system for studying the dynamics of metabolic switching in living systems. In an open-flow system the oscillations can be maintained indefinitely at a constant operating point where they can....... Experimental and computational results strongly suggest that regulation of acetaldehyde explains the observed behavior. We have extended the original model with regulation of pyruvate decarboxylase, a reversible alcohol dehydrogenase, and drainage of pyruvate. Using the method of time rescaling in the extended...

  8. Restructuring of microparticles

    International Nuclear Information System (INIS)

    Lameiras, F.S.; Santos, A.M.M. dos

    1992-01-01

    Experimental grain sizes distribution of sintered (U,Gd)O 2 pellets were analysed according to the model of Lameiras for microparticles restructuring. This model, which includes the grain growth and Ostwald ripening phenomena, assumes that the microparticles restructuring is governed by two fundamental principles: minimization of the interface energy and uniformization of its distribution in space. It is also, assumed that the interface energy is stored in the grain boundaries, triple lines and quadruple points. The minimization of the interface energy can be done through three ways independent of each other: diminishing of the number of microparticles, alteration of the size distribution and alteration of the form distribution. The uniformization of the spatial distribution of the interface energy can be done through two ways also independent of each other: tendency to an uniform spatial distribution of microparticles and tendency to an uniform distribution of the interface energy per microparticle. The model accords well with these experimental data. (author)

  9. Perspectives on industry restructuring

    International Nuclear Information System (INIS)

    Konow, H. R.

    1996-01-01

    An industry association perspective on restructuring in the electrical industry was offered, placing the ongoing debate about deregulation and competition in the industry in Ontario into a Canadian and a global context. It was found that the move towards deregulation was a global phenomenon, fuelled by the emergence of increasingly open and highly competitive global markets, heightened consumer expectations, and new technologies and convergence. Examples from the U.K., Norway, Australia, New Zealand and the United States were cited to indicate the spread of this trend. Canadian utilities, among them B.C. Hydro, Nova Scotia Power and the Alberta utilities being prime examples, also have restructured recently into separate generation, transmission, distribution and consumer service units to better react to changing market needs. Alliances with other utilities or competitors in the energy sector to pursue business opportunities outside traditional markets, are also not uncommon. Therefore, it was only a question of time for Ontario Hydro, the largest Canadian electric utility, to come under public pressure to follow the national and global trend. The MacDonald Committee recommendations provide the foundation for radical change for the industry. If and when implemented, they could have a major impact on how the electricity sector in Canada will operate in the years to come. Sound decisions by the Ontario government as to the appropriate regulatory model may also determine the province's ability to compete in the unfolding restructuring of electricity markets throughout North America

  10. Electric industry restructuring review

    International Nuclear Information System (INIS)

    Slocum Hollis, S.

    2004-01-01

    Restructuring of the electric power industry began in the early 1990's in many jurisdictions in the United States. Restructuring was an attempt to offer large industrial customers lower rates and freedom from regulation for generators and traditional public utilities. The move has gained most attention in the past two years as some utilities report high profits while others, such as Pacific Gas and Electric Co., the largest investor-owned utility in the United States, is in bankruptcy. The August 2003 blackout in the Midwest and Northeast United States and Canada also raised questions regarding electric reliability. The question now remains whether markets should be allowed to determine the need for services and the prices to be charged, and who is in charge in the imperfect market. The Federal Energy Regulatory Commission's (FERC) Order 2000 led to the formation of a Regional Transmission Organizations which is still in the implementation stage. Its influence on precursor Order numbers 888 and 889 were discussed in this paper with reference to independent system operators; regional transmission organizations; standard market design; rates and pricing devices; congestion management; market monitoring; market investigations; reliability measures; OASIS and other information access; interconnection policy; jurisdiction; mergers and merger policy; standards of conduct; policing affiliates; municipal utilities; stranded costs; and, state restructuring scorecards. refs

  11. Sharing China's Bank Restructuring Bill

    Institute of Scientific and Technical Information of China (English)

    Guonan Ma

    2006-01-01

    This paper addresses the questions related to the cost of China's bank restructuring and how it has been financed. We first propose a framework for recognizing losses. Then, we examine the recent major moves by the Chinese Government to repair the country's bank balance sheets. Finally, we explore the implications of the Chinese Government's methods of funding bank restructuring. We find that the Chinese Government has been decisive in confronting the costly task of bank restructuring. So far, Chinese taxpayers have paid most of the bill for bank restructuring.

  12. Restructuring Restructuring: Postmodernity and the Prospects for Educational Change.

    Science.gov (United States)

    Hargreaves, Andy

    Change by school restructuring has followed quickly on the heels of school reform that sought to mandate improvement upon teachers by bureaucratic control and compliance. It did not take long for problems of the reform paradigm to surface as teacher improvement could not be mandated. A complete restructuring of teaching and learning organization…

  13. Glycolytic inhibitors 2-deoxyglucose and 3-bromopyruvate synergize with photodynamic therapy respectively to inhibit cell migration.

    Science.gov (United States)

    Feng, Xiaolan; Wang, Pan; Liu, Quanhong; Zhang, Ting; Mai, Bingjie; Wang, Xiaobing

    2015-06-01

    Most cancer cells have the specially increased glycolytic phenotype, which makes this pathway become an attractive therapeutic target. Although glycolytic inhibitor 2-deoxyglucose (2-DG) has been demonstrated to potentiate the cytotoxicity of photodynamic therapy (PDT), the impacts on cell migration after the combined treatment has never been reported yet. The present study aimed to analyze the influence of glycolytic inhibitors 2-DG and 3-bromopyruvate (3-BP) combined with Ce6-PDT on cell motility of Triple Negative Breast Cancer MDA-MB-231 cells. As determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium-bromide-Tetraz-olium (MTT) assay, more decreased cell viability was observed in 2-DG + PDT and 3-BP + PDT groups when compared with either monotherapy. Under optimal conditions, synergistic potentiation on cell membrane destruction and the decline of cell adhesion and cells migratory ability were observed in both 2-DG + PDT and 3-BP + PDT by electron microscope observation (SEM), wound healing and trans-well assays. Besides, serious microfilament network collapses as well as impairment of matrix metalloproteinases-9 (MMP-9) were notably improved after the combined treatments by immunofluorescent staining. These results suggest that 2-DG and 3-BP can both significantly potentiated Ce6-PDT efficacy of cell migration inhibition.

  14. Insider Privatisation and Restructuring Incentives

    DEFF Research Database (Denmark)

    Schröder, Philipp

    2003-01-01

    In the literature on privatisation and restructuring it is a generally held belief that manager owned firms will be restructured more rigorously than worker owned companies. This gives the clear recommendation that property rights and control rights should be allocated to managers in the process...

  15. Theoretical Grounds of Enterprise Restructuring

    Directory of Open Access Journals (Sweden)

    Odintsova Nadiya G.

    2013-11-01

    Full Text Available One of the most popular means of financial recovery of enterprises in the world and domestic theory and practice is re-structuring. The article considers the essence of enterprise re-structuring, conducts analysis of existing approaches to understanding the re-organisation, re-formation, re-structuring and re-engineering notions and makes conclusions with respect to the nature of their interconnection. It analyses interpretation of the re-structuring category, approaches of scientists to revelation of the essence of the enterprise re-structuring notion. To achieve the goal the article conducts assessment of the existing approaches to interpretation of the content of the enterprise re-structuring process. It also conducts further analysis of interconnection of the re-formation, re-structuring, re-organisation and re-engineering notions with consideration of scientific interpretations, which would allow identification of the roles of each of the notions for identifying their influence and interconnection in correlation with the direction of economic activity of enterprises.

  16. Electricity restructuring : a comparative review. 2. ed.

    International Nuclear Information System (INIS)

    Trebilcock, M.J.

    2004-03-01

    Power generation, transmission and distribution are the 3 main components of the electricity market. For many years, most jurisdictions chose to vertically integrate these segments into government or private monopolies. Price controls or rate of return regulations were commonly imposed by governments to prevent the abuse of monopoly power. However, inefficiencies with these arrangements led to poor investment decisions. As a result, many jurisdictions re-evaluated the structure of electricity markets. Restructuring was motivated by the desire to shift investment risk from consumers or taxpayers to producers and investors. The introduction of competitive electricity generation markets created a new sector in the industry. This sector was the retailing or selling of electricity to end-users by retail intermediaries. Although restructuring of the industry should offer more efficient pricing and better-informed consumption and investment decisions, there are many challenges in electricity reform. These challenges include pricing by incumbent generators, transmitters and distributors; discriminatory network access by monopoly transmitters and distributors; inelasticity of electricity supply and demand at peak times; lack of real-time price notification and response by consumers; stranded costs; and, political and consumer resistance to increases in retail prices. The time it takes to get a new power plant online is another issues that complicates new entry in the generation sector. The results of restructuring in Ontario, California, United Kingdom, Pennsylvania, New Jersey, Maryland, Alberta, and Australia were examined. These jurisdictions are trying to find a balance between competition and regulation. 91 refs., 2 figs

  17. Restructuring Teachers' Work

    Directory of Open Access Journals (Sweden)

    Lisa Kirtman

    2002-05-01

    Full Text Available Despite repeated attempts to reform schools, teachers' work has remained surprisingly stable. The purpose of this study was to investigate implementation of a state-funded restructuring initiative that intended broad changes in teachers' professional roles. Sponsors of the founding legislation reasoned that changes in teachers' roles would contribute to higher student achievement. This study examined the question of whether and how this program of comprehensive whole-school change promoted changes in teachers' roles in school governance, collegial relations, and the classroom. Further, the study traced the relationship of these changes to one another, and weighed the likelihood that they had the capacity to affect core educational practices. Theoretically, this study is situated in the available literature on teachers' collegial relations; participation in shared decision making; and classroom roles, relationships and practice. Three elementary schools served as the sites for intensive qualitative data collection completed over a two-year period. The schools differed in geographic location (two urban, one rural, but all enrolled a racially, ethnically and linguistically diverse population of students, and more than half of the students in each school qualified for free or reduced price lunch. The study resulted in multiple types and sources of data on teachers' professional roles, including: observations in classrooms, collegial interactions, and governance situations; interviews with teachers (including teacher leaders, parents, administrators, and students; and documents pertaining to the restructuring plans and process. Findings show that changes in the three areas were achieved unevenly in the three schools. All three schools introduced changes in classroom practice and roles, ranging from the adoption of multi-age classrooms to more modest innovations in curriculum or instruction. In only one case were changes in professional roles outside

  18. Bulgarian electricity market restructuring

    International Nuclear Information System (INIS)

    Ganev, Peter

    2009-01-01

    The energy sector in Bulgaria has undergone major restructuring in recent years. It faces the dual challenges of achieving regulatory stability to attract private investors, and creating a functioning competition energy market. As of the EU Accession in 2007, Bulgaria has fully liberalized power and gas markets. The 2003 Energy Law establishes the energy sector legal framework and sets the basis for creation of a transparent and predictable regulatory environment where the key regulatory responsibilities are vested with the State Energy and Water Regulatory Commission (SEWRC). The energy sector experienced significant problems in the first half of 2007 due to lost production capacities and regulatory failures on the electricity market. Excess price regulations on the market of electricity supplies to household, coupled with insufficient liberalization of imports and exports, create unfavorable conditions for power producers and large electricity users. The energy regulator has tried to achieve several incompatible targets as of July 1, 2007 for maintaining low electricity prices for households in response to political pressure, low power generation prices amid rising input costs, and market opening in compliance with EU regulations. (author)

  19. DSM in restructured jurisdictions

    Energy Technology Data Exchange (ETDEWEB)

    Fraser, M.

    1996-08-01

    The impact of regulatory restructuring on demand side management (DSM) was reviewed, drawing on the experiences gained in Norway, England and New Zealand, with a view to determining alternative motivators for energy efficiency in the absence of regulations. The implications of deregulation were examined. It was found that each of the three countries was forced to return to the question of DSM and energy services programs, and the role of utilities. Evidence seemed to indicate that with the removal of the shielding effect of regulation on the utilities generation market, the difference in investment payback between generators and suppliers was likely to approach the levels that exist in other segment of the economy, thus pricing market failure is likely to be removed. However, other market failures may well remain, such as (1) attention to environmental externalities, (2) consumers` inability to influence the efficiency or use of energy consuming equipment, (3) consumers` difficulty in acquiring information on efficiency measures, and in implementing such measures. In view of the Rio de Janeiro obligations policy makers will have to find market mechanisms to remedy these shortcomings. 16 refs.

  20. Trademark Values in Corporate Restructuring

    OpenAIRE

    Torres, Fernando

    2007-01-01

    In corporate restructuring under Chapter 11, an asset valuation is a central task for both legal and financial reasons. In the area of intangible assets, however, generally accepted accounting principles (GAAP) do not reflect internally-generated assets such as brands, trademarks, and other intellectual property. In practice, arbitrary rules of thumb are used to fill this gap, and closure, liquidation, financing, and restructuring decisions are made on this basis. This paper reports the ...

  1. Glycolytic Enzymes Coalesce in G Bodies under Hypoxic Stress

    Directory of Open Access Journals (Sweden)

    Meiyan Jin

    2017-07-01

    Full Text Available Glycolysis is upregulated under conditions such as hypoxia and high energy demand to promote cell proliferation, although the mechanism remains poorly understood. We find that hypoxia in Saccharomyces cerevisiae induces concentration of glycolytic enzymes, including the Pfk2p subunit of the rate-limiting phosphofructokinase, into a single, non-membrane-bound granule termed the “glycolytic body” or “G body.” A yeast kinome screen identifies the yeast ortholog of AMP-activated protein kinase, Snf1p, as necessary for G-body formation. Many G-body components identified by proteomics are required for G-body integrity. Cells incapable of forming G bodies in hypoxia display abnormal cell division and produce inviable daughter cells. Conversely, cells with G bodies show increased glucose consumption and decreased levels of glycolytic intermediates. Importantly, G bodies form in human hepatocarcinoma cells in hypoxia. Together, our results suggest that G body formation is a conserved, adaptive response to increase glycolytic output during hypoxia or tumorigenesis.

  2. Glycolytic overload and the genesis of breast cancer.

    Science.gov (United States)

    Robson, J R

    1984-03-01

    It is suggested that the development of breast cancer is due to overloading of the glycolytic pathways. An excess of substrates or an excessive delivery rate of substrates to the Krebs Cycle is believed to result in the formation of acetyl CoA. Feedback mechanisms controlling the conversion of acetyl CoA to cholesterol may be overcome; the resulting high concentration of cholesterol induces the formation of pregnenolone which may then be converted into androgens, estrogens and progesterone. These steroids are in addition to those produced by gonads and adrenal glands. Glycolytic overload is also associated with an increase in fat stores which have been shown to be the site of interconversion of sex hormones. Excess sex hormones or abnormal sex hormones are believed to be the cause of breast cancer. The hypothesis presented links glycolytic overload with clinical biochemical phenomena and explains some of the anomalies observed in breast cancer experience in different ethnic groups. Changes in dietary habits during the history of man resulting in " gorging " and the consumption of highly refined sugars are possible causes of glycolytic overload. So, also, is impaired thermogenesis due to Brown Fat deficits in certain ethnic groups.

  3. Study of glycolytic intermediates in hereditary elliptocytosis with thalassemia

    Directory of Open Access Journals (Sweden)

    Pavri Roshan

    1977-01-01

    Full Text Available Glycolytic intermediates like ATP, DPG and GSH have been studied in a family with. hereditary elliptocytosis and thalassemia. Results indicate a fall in ATP with a concomitant rise in DPG in the Patient. Findings are discussed in relation to other data.

  4. The glycolytic pathway of Trimastix pyriformis is an evolutionary mosaic

    Directory of Open Access Journals (Sweden)

    Silberman Jeffrey D

    2006-11-01

    Full Text Available Abstract Background Glycolysis and subsequent fermentation is the main energy source for many anaerobic organisms. The glycolytic pathway consists of ten enzymatic steps which appear to be universal amongst eukaryotes. However, it has been shown that the origins of these enzymes in specific eukaryote lineages can differ, and sometimes involve lateral gene transfer events. We have conducted an expressed sequence tag (EST survey of the anaerobic flagellate Trimastix pyriformis to investigate the nature of the evolutionary origins of the glycolytic enzymes in this relatively unstudied organism. Results We have found genes in the Trimastix EST data that encode enzymes potentially catalyzing nine of the ten steps of the glycolytic conversion of glucose to pyruvate. Furthermore, we have found two different enzymes that in principle could catalyze the conversion of phosphoenol pyruvate (PEP to pyruvate (or the reverse reaction as part of the last step in glycolysis. Our phylogenetic analyses of all of these enzymes revealed at least four cases where the relationship of the Trimastix genes to homologs from other species is at odds with accepted organismal relationships. Although lateral gene transfer events likely account for these anomalies, with the data at hand we were not able to establish with confidence the bacterial donor lineage that gave rise to the respective Trimastix enzymes. Conclusion A number of the glycolytic enzymes of Trimastix have been transferred laterally from bacteria instead of being inherited from the last common eukaryotic ancestor. Thus, despite widespread conservation of the glycolytic biochemical pathway across eukaryote diversity, in a number of protist lineages the enzymatic components of the pathway have been replaced by lateral gene transfer from disparate evolutionary sources. It remains unclear if these replacements result from selectively advantageous properties of the introduced enzymes or if they are neutral

  5. ­La restructuration du réseau culturel français au quotidien The everyday reconstruction of French cultural network La reestructuración cotidiana de la red cultural francesa

    Directory of Open Access Journals (Sweden)

    Christophe Premat

    2013-03-01

    Full Text Available Il s’agit de comprendre les transformations des conditions du métier d’agent culturel à la lumière des réformes en cours du réseau culturel français. La restructuration des activités culturelles des Instituts est-elle le signe d’une privatisation de l’action culturelle extérieure de l’État ? La coopération culturelle serait-elle alors prise en charge par d’autres acteurs ? Afin de répondre à ces questions, l’article s’appuie sur une étude de cas, celle de l’Institut français de Stockholm, qui a abandonné une partie de ses activités au profit d’autres actions de coopération.The article tries to understand how current reforms of the French cultural network might transform the profession of cultural agent. The question is whether the restructuring of cultural institutes’ cultural activities reflects a privatization of the State’s external cultural action, and if other actors might then assume responsibility for cultural cooperation. It responds with a case study of the French cultural centre in Stockholm, which gave up a part of its activities to adopt other cooperation actions.En este trabajo tratamos de comprender las transformaciones de las condiciones del oficio de agente cultural a la luz de las reformas actuales en la red cultural francesa. ¿Es la reestructuración de las actividades culturales de los Institutos el signo de una privatización de la acción cultural exterior del Estado ? ¿Otros actores tomarían entonces en sus manos la cooperación cultural ? Con el fin de responder a esas preguntas, el artículo se apoya en un estudio de caso realizado por el Instituto francés de Estocolmo, el cual abandonó una parte de sus actividades para encargarse de otras acciones de cooperación.

  6. A chemometric method to identify enzymatic reactions leading to the transition from glycolytic oscillations to waves

    Science.gov (United States)

    Zimányi, László; Khoroshyy, Petro; Mair, Thomas

    2010-06-01

    In the present work we demonstrate that FTIR-spectroscopy is a powerful tool for the time resolved and noninvasive measurement of multi-substrate/product interactions in complex metabolic networks as exemplified by the oscillating glycolysis in a yeast extract. Based on a spectral library constructed from the pure glycolytic intermediates, chemometric analysis of the complex spectra allowed us the identification of many of these intermediates. Singular value decomposition and multiple level wavelet decomposition were used to separate drifting substances from oscillating ones. This enabled us to identify slow and fast variables of glycolytic oscillations. Most importantly, we can attribute a qualitative change in the positive feedback regulation of the autocatalytic reaction to the transition from homogeneous oscillations to travelling waves. During the oscillatory phase the enzyme phosphofructokinase is mainly activated by its own product ADP, whereas the transition to waves is accompanied with a shift of the positive feedback from ADP to AMP. This indicates that the overall energetic state of the yeast extract determines the transition between spatially homogeneous oscillations and travelling waves.

  7. The unique features of glycolytic pathways in Archaea.

    Science.gov (United States)

    Verhees, Corné H; Kengen, Servé W M; Tuininga, Judith E; Schut, Gerrit J; Adams, Michael W W; De Vos, Willem M; Van Der Oost, John

    2003-10-15

    An early divergence in evolution has resulted in two prokaryotic domains, the Bacteria and the Archaea. Whereas the central metabolic routes of bacteria and eukaryotes are generally well-conserved, variant pathways have developed in Archaea involving several novel enzymes with a distinct control. A spectacular example of convergent evolution concerns the glucose-degrading pathways of saccharolytic archaea. The identification, characterization and comparison of the glycolytic enzymes of a variety of phylogenetic lineages have revealed a mosaic of canonical and novel enzymes in the archaeal variants of the Embden-Meyerhof and the Entner-Doudoroff pathways. By means of integrating results from biochemical and genetic studies with recently obtained comparative and functional genomics data, the structure and function of the archaeal glycolytic routes, the participating enzymes and their regulation are re-evaluated.

  8. The unique features of glycolytic pathways in Archaea.

    OpenAIRE

    Verhees, Corné H; Kengen, Servé W M; Tuininga, Judith E; Schut, Gerrit J; Adams, Michael W W; De Vos, Willem M; Van Der Oost, John

    2003-01-01

    An early divergence in evolution has resulted in two prokaryotic domains, the Bacteria and the Archaea. Whereas the central metabolic routes of bacteria and eukaryotes are generally well-conserved, variant pathways have developed in Archaea involving several novel enzymes with a distinct control. A spectacular example of convergent evolution concerns the glucose-degrading pathways of saccharolytic archaea. The identification, characterization and comparison of the glycolytic enzymes of a vari...

  9. Electricity restructuring : acting on principles

    International Nuclear Information System (INIS)

    Down, E.; Hoover, G.; Howatson, A.; Rheaume, G.

    2003-01-01

    In the second briefing of this series, the authors explored public policy decisions and political intervention, and their effect on electricity restructuring. Continuous and vigilant regulatory oversight of the electricity industry in Canada is required. The need for improved public policy to reduce uncertainty for private investors who wish to enter the market was made clear using case studies from the United Kingdom, California, Alberta, and Ontario. Clarity and consistency must be the two guiding principles for public policy decisions and political intervention in the sector. By clarity, the authors meant that rules, objectives, and timelines of the restructuring process are clear to all market participants. Market rules, implementation, and consumer expectations must be consistent. refs., 3 figs

  10. On Restructurable Control System Theory

    Science.gov (United States)

    Athans, M.

    1983-01-01

    The state of stochastic system and control theory as it impacts restructurable control issues is addressed. The multivariable characteristics of the control problem are addressed. The failure detection/identification problem is discussed as a multi-hypothesis testing problem. Control strategy reconfiguration, static multivariable controls, static failure hypothesis testing, dynamic multivariable controls, fault-tolerant control theory, dynamic hypothesis testing, generalized likelihood ratio (GLR) methods, and adaptive control are discussed.

  11. Development and operation of interconnections in a restructuring context

    International Nuclear Information System (INIS)

    2003-01-01

    In many countries the electrical network is not fully interconnected and the best technical solution to achieve interconnection has to be found. At the same time the electricity industry is being restructured and interconnecting independent energy markets presents technical challenges. It is therefore timely to consider interconnection development and operation options: examine the benefits of interconnecting electrical networks and the development strategies, review the interconnection design options and the technologies available, identify the operational issues, the security problems of large interconnected systems, the protection issues, consider the impact of the restructuring of the electrical supply industry, assess the political, environmental and social implications of interconnections. reorganized in slovenia from 5-7 april 2004. (author)

  12. Essays on restructured electricity markets

    Science.gov (United States)

    Nicholson, Emma Leah

    This dissertation focuses on the performance of restructured electricity markets in the United States. In chapter 1, I study bidder-specific offer caps ("BSOCs") which are used to mitigate market power in three wholesale electricity markets. The price of electricity is determined through multi-unit uniform price auctions and BSOCs impose an upper limit, which is increasing in marginal cost, on each generator's bid. I apply BSOCs in both the uniform and discriminatory price auctions and characterize the equilibria in a two firm model with stochastic demand. BSOCs unambiguously increase expected production efficiency in the uniform price auction and they can increase the expected profit of the generator with the lower cap. Chapter 2, coauthored with Ramteen Sioshansi, Ph.D., compares two types of uniform price auction formats used in wholesale electricity markets, centrally committed markets and self committed markets. In centrally committed markets, generators submit two-part bids consisting of a fixed startup cost and a variable (per MWh) energy cost, and the auctioneer ensures that no generator operates at a loss. Generators in self committed markets must incorporate their startup costs into their one part energy bids. We derive Nash equilibria for both the centrally and self committed electricity markets in a model with two symmetric generators with nonconvex costs and deterministic demand. Using a numerical example, we demonstrate that if the caps on the bid elements are chosen appropriately, the two market designs are equivalent in terms of generator revenues and settlement costs. Regulators and prominent academic experts believe that electric restructuring polices have stifled investment in new generation capacity. In chapter 3 I seek to determine whether these fears are supported by empirical evidence. I examine both total investment in megawatts and the number of new investments across regions that adopted different electric restructuring policies to

  13. Role of Glycolytic Intermediates in Global Regulation and Signal Transduction. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Liao, J.C.

    2000-05-08

    The goal of this project is to determine the role of glycolytic intermediates in regulation of cell physiology. It is known that many glycolytic intermediates are involved in regulation of enzyme activities at the kinetic level. However, little is known regarding the role of these metabolites in global regulation and signal transduction. This project aims to investigate the role of glycolytic intermediates in the regulation of gene expression.

  14. How restructuring river connectivity changes freshwater fish biodiversity and biogeography

    Science.gov (United States)

    Lynch, Heather L.; Grant, Evan H. Campbell; Muneepeerakul, Rachata; Arunachalam, Muthukumarasamy; Rodriguez-Iturbe, Ignacio; Fagan, William F.

    2011-01-01

    Interbasin water transfer projects, in which river connectivity is restructured via man-made canals, are an increasingly popular solution to address the spatial mismatch between supply and demand of fresh water. However, the ecological consequences of such restructuring remain largely unexplored, and there are no general theoretical guidelines from which to derive these expectations. River systems provide excellent opportunities to explore how network connectivity shapes habitat occupancy, community dynamics, and biogeographic patterns. We apply a neutral model (which assumes competitive equivalence among species within a stochastic framework) to an empirically derived river network to explore how proposed changes in network connectivity may impact patterns of freshwater fish biodiversity. Without predicting the responses of individual extant species, we find the addition of canals connecting hydrologically isolated river basins facilitates the spread of common species and increases average local species richness without changing the total species richness of the system. These impacts are sensitive to the parameters controlling the spatial scale of fish dispersal, with increased dispersal affording more opportunities for biotic restructuring at the community and landscape scales. Connections between isolated basins have a much larger effect on local species richness than those connecting reaches within a river basin, even when those within-basin reaches are far apart. As a result, interbasin canal projects have the potential for long-term impacts to continental-scale riverine communities.

  15. Network development plan 1995

    International Nuclear Information System (INIS)

    Anon.

    1995-11-01

    Network plan 1995 concerns several strategic problems, among others environmental policy of power transmission lines. Possibilities of restructuring aerial cable network are described. The state of the existing systems and plans for new network systems are presented. (EG)

  16. United States electric industry : restructuring in review

    International Nuclear Information System (INIS)

    Slocum Hollis, S.

    2004-01-01

    This paper provides a detailed review of the United States electric power industry. The aim of the review was to clarify and better define current industry procedures and practices in light of significant and recent restructuring. In addition, recent bankruptcies and the power blackout in 2003 have raised concerns over industry practices. Issues concerning Independent System Operators (ISO) and regional transmission organizations were evaluated, with reference to an evolution and implementation of Regional Transmission Organization (RTO) policy, including a cost-benefit analysis. A background of RTO formations was provided with reference to consolidation, selection process and transfer of assets. Standard market design, network access and pricing issues were reviewed, as well as market and reliability concerns. Issues concerning affiliate treatment, shortages and the effect of sale of securities were presented. Various approaches to congestion management were examined, with examples from California and New England. Market monitoring issues, investigations and hearings were also examined, with examples and orders, including details of refunds. Measures to improve reliability were reviewed, including: management systems, benefit margins, requirements, assurance agreements and reserve markets. Issues concerning information access were presented, including: Open Access Same-time Information System (OASIS) requirements; tagging; standard business practices and protocols; and quarterly report practices and protocols. Interconnection policies were reviewed with reference to applicability, service options and pricing. The issue of variations was examined, with case examples concerning cost allocation, contract rights and treatment of specific costs. Jurisdiction issues concerning corporate realignments and power exchanges were presented, as well as specific services and state-federal relations. Issues concerning mergers and merger policy were also discussed, with reference

  17. Coasts under stress: restructuring and social-ecological health

    National Research Council Canada - National Science Library

    Ommer, Rosemary E

    2007-01-01

    ... the human impact of restructuring and social-ecological health 9 The Restructuring of Health Care on Both Coasts since the 1980 s 183 The Statistical Face of Restructuring and Human Health 210 The Human Voice of Social-Ecological Restructuring: Jobs, Incomes, Livelihoods, Ways of Life, and Human Health 241vi Contents 10 11 Restructuring, Nutrition,...

  18. Social responsibility and SOE restructuring

    Institute of Scientific and Technical Information of China (English)

    沈志渔; 刘兴国; 周小虎

    2009-01-01

    SOE social responsibility has undergone three stages of evolution.In essence,corporate social responsibility includes social obligations and social expectations.Public attention to SOE social responsibility issues has affected the thinking surrounding SOE restructuring,including the promulgating of objectives and methods.Based on corporate social responsibility,SOE managers must set up a perfect SOE social responsibility system and strengthen supervisory mechanisms;in respect to corporate governance models,SOEs should undertake reform for the corporate citizen governance model.

  19. Electric industry restructuring in Michigan

    International Nuclear Information System (INIS)

    Anon.

    1997-01-01

    This Staff Report suggests a modified approach designed to significantly increase the ability of all customer classes to participate and share in the benefits of competition. The concepts discussed in this Report are designed to ensure that rates are not increased for any customers as a result of restructuring and, where possible, rates are reduced through the use of rate reduction bonds. The program outlined in this Report is designed to fulfill five objectives. First, it protects the interests of smaller customers, including low-income residential customers and senior citizens. Second, the program provides opportunities to strengthen Michigan's business community. Third, the program includes funding for employee retraining to assure that utility employees are not negatively impacted by restructuring. Fourth, the phase-in program provides the utilities with the opportunity to prepare for competition so that they remain Michigan-based companies. Fifth, the program is designed to foster competition upon a level playing field. The Commission has jurisdiction over all investor electric utilities and rural electric cooperatives in Michigan. Municipal electric utilities are not subject to Commission jurisdiction. Although this Report discusses details regarding Consumers Power and Detroit Edison, its concepts and principles are intended to apply to all jurisdictional electric utilities

  20. Electricity restructuring: charting new frontiers

    International Nuclear Information System (INIS)

    Kwaczek, A.

    1996-01-01

    Various aspects of restructuring in the electrical industry were examined. A general warning was issued to the effect that there are no 'one-size-fits-all' answers to competing in the markets that will follow deregulation. Experiences with electricity deregulation in other countries, such as the U.K., Argentina, Chile, New Zealand, Australia, Sweden, Norway, and more recently, in Alberta, were described. Two major lessons emerged: (1) there are a number of ways to achieve the same goal, and (2) different jurisdictions have different goals, i.e. instead of deregulation being a universal process that will lead to the correct destination, restructuring in each province of Canada will more likely evolve through a series of tradeoffs among objectives. The final outcome will be determined by policy decisions about issues such as efficiency versus equity, public versus private, and big versus small. The most important issues that each jurisdiction will have to address have been identified as market power in both generation and system operation, pooling, and the extent of competition. It was also stated that while the issues are complex, they are not intractable; all experience shows that the transition is manageable, and sometimes even exciting

  1. A restructuring proposal for MIDAS

    International Nuclear Information System (INIS)

    Park, S. H.; Song, Y. M.; Kim, D. H.

    2000-01-01

    In order to develop a template for the MIDAS computer code, current data saving and transferring methods used in MELCOR are addressed first, and then a restructured module for the SPR package has been developed to be applied to MELCOR. The current MELCOR code ensures a fixed-size storage for four different data types, and manages the input-dependent data size by storing the data on the stacked packages. It uses pointer to identify the variables between the packages. This technique causes a difficult grasping of the meaning of the variables as well as waste of memory. New features of FORTRAN90, however, make it possible to allocate the storage dynamically and to use the user-defined data type, which lead to a restructured module development for the SPR package. An efficient memory treatment and an easy understanding of the code are the benefit from this developed module. The validation of the template has been done by comparing the results of the modified code with those from the existing code, and it is confirmed that the results are the same. The template for the SPR package hints the extension of the template to the entire code. It is expected that the template will accelerate the code domestication thanks to direct understanding of each variable and easy implementation of modified or newly developed models

  2. An Analysis of Policy Issues in Natural Gas Industry Restructuring

    Energy Technology Data Exchange (ETDEWEB)

    Seo, J.K. [Korea Energy Economics Institute, Euiwang (Korea)

    2001-12-01

    This report explores controversial issues and problems that could emerge in the process of implementing the government's restructuring plan for natural gas industry and aims to suggest policy directions regarding the restructuring. To begin with, it examines current conditions surrounding natural gas industry including domestic demand and supply conditions, world LNG market trend, structural changes of the industry in OECD countries, possibilities of introducing effective competition and assignment of existing import contracts. In doing so, we probe whether the direction of the natural gas industry restructuring is proper and suggest that the results be reflected when a more detailed restructuring implementation plan is formulated. Also, this report suggests possible schemes related to major institutional changes expected by the basic restructuring plan and the detailed restructuring implementation plan announced by the government. First, it presents several alternative ways to properly divide up the import/wholesales section of Korea Gas Corporation. Second, it examines critical issues such as the method of using gas supply facilities and gas balancing mechanism, and presents adoptable alternatives for each issue. These issues constitute the core of network and market operation rules which need to be in place when adopting an open access system, which in turn is a prerequisite for sales competition. Third, the report examines price systems, including gas commodity pricing and rate-making design for transportation service, by first anticipating the direction of changes in gas rate regulation. Specifically, it presents possible ways to design the service rate for each function of pipeline network and import terminals, and discusses controversial issues in determining total cost-of-service and allocating the cost-of-service to each functional service offered such as distance-related rates, interruptible service rate. Lastly, in relation to the opening of retail

  3. RESTRUCTURING INDONESIAN RAILWAY – INTEGRATION OR SEPARATION

    Directory of Open Access Journals (Sweden)

    Utut Widyanto

    2013-05-01

    The study found that the separation model is still the best approach for restructuring Indonesian railway but if looking at the Indonesian railway current condition with its problem of backlog assets it would be better that the separation approach is used in the development of railway in other islands. Keywords: Restructuration, separation, funds, operator.

  4. Reduction of Test Anxiety Through Cognitive Restructuring

    Science.gov (United States)

    Goldfried, Marvin R.; And Others

    1978-01-01

    On the basis of questionnaire measures of test anxiety, only those in the rational restructuring condition reported a significant decrease in subjective anxiety when placed in an analogue test-taking situation. Participants in the restructuring condition also reported greater generalized anxiety reduction in social-evaluative situations. (Author)

  5. Scaling of oxidative and glycolytic enzymes in mammals.

    Science.gov (United States)

    Emmett, B; Hochachka, P W

    1981-09-01

    The catalytic activities of several oxidative and glycolytic enzymes were determined in the gastrocnemius muscle of 10 mammalian species differing in body weight by nearly 6 orders of magnitude. When expressed in terms of units gm-1, the activities of enzymes functioning in oxidative metabolism (citrate synthase, beta-hydroxybutyrylCoA dehydrogenase, and malate dehydrogenase) decrease as body weight increases. Log-log plots (activity gm-1 vs body mass) yield straight lines with negative slopes that are less than the allometric exponent (-0.25) typically observed for basal metabolic rates. Since the amount of power a muscle can generate depends upon the catalytic potential of its enzyme machinery (the higher the catalytic potential the higher the maximum rate of energy generation), these data predict that the scope for aerobic activity in large mammals should be greater than in small mammals if nothing else becomes limiting, a result in fact recently obtained by Taylor et al. (Respir. Physiol., 1981). In contrast to the scaling of oxidative enzymes, the activities of enzymes functioning in anaerobic glycogenolysis (glycogen phosphorylase, pyruvate kinase, and lactate dehydrogenase) increase as body size increases. Log-log plots (activity gm-1 vs body mass) display a positive slope indicating that the larger the animal the higher the glycolytic potential of its skeletal muscles. This unexpected result may indicate higher relative power costs for burst type locomotion in larger mammals, which is in fact observed in within-species studies of man. However, the scaling of anaerobic muscle power has not been closely assessed in between-species comparisons of mammals varying greatly in body size.

  6. Romanian refining industry assesses restructuring

    International Nuclear Information System (INIS)

    Tanasescu, D.G.

    1991-01-01

    The Romanian crude oil refining industry, as all the other economic sectors, faces the problems accompanying the transition from a centrally planned economy to a market economy. At present, all refineries have registered as joint-stock companies and all are coordinated and assisted by Rafirom S.A., from both a legal and a production point of view. Rafirom S.A. is a joint-stock company that holds shares in refineries and other stock companies with activities related to oil refining. Such activities include technological research, development, design, transportation, storage, and domestic and foreign marketing. This article outlines the market forces that are expected to: drive rationalization and restructuring of refining operations and define the targets toward which the reconfigured refineries should strive

  7. Universal informations of restructuralization of magnesite firm

    Directory of Open Access Journals (Sweden)

    Bandurová Miriam

    2001-12-01

    Full Text Available The article presents general process of the judging of organization structures. At beginning there are defined development trends in the organizational structures, form of cooperation and concentration of the firm and of enterprise network. In present time it is very actual questions of organization of large company in the world. Then strategy of organization is described which can be classical and elastic. From new expression in this area is writed reengineering as a principle and radical reconstruction of company process. Between other forms of existence of firms (companies in this time belongs their combination and its forms there are described in this article too.The basic of contribution consists of informations about mining industry in the Slovakia and restructuralization of mining companies after 1989. There is mentioned proposal on general process at analysis of organizational structure of magnesite firm. The basic at the analysis is existence united model of every company produced in this area. Model presents division of organization to two parts: production and administration part. After analysis of individual production process in the production part and technical workers of administration parts of organization there are suggested two alternatives on solution of these problems.

  8. The restructuring of Africa's electricity sector : lessons learned

    International Nuclear Information System (INIS)

    Fall, A.

    2008-01-01

    The challenges facing the electricity sector in Africa was discussed with particular reference to financial constraints and the inefficient management of public utilities. The 2 types of reform in the electricity sector include the disengagement of the State for the benefit of the private sector, and the restructuring of the electrical industry to include competition for greater efficiency in the sector. The first reform in Africa involved the opening of the sector to private enterprises without the restructuring of the industry. With the exception of Uganda, the evolution of the restructuring did not include a complete de-integration in the transitional stage. Restructuring involved systematic independent production; a single buyer model with a limited period of monopoly on bulk purchase; access to third party on the networks; empowerment of regulation by the creation of an independent body responsible for regulation; and, establishment of a rural electrification agency. In more advanced countries such as Egypt, Algeria and Nigeria, the creation of a holding company with legally separated subsidiaries was retained. One of the major problems was financial investment, technical performances and the extension of electrical access. Low competition, or low negotiation capabilities led to unbalanced contracts in favour of private promoters. In general, rates for electrical energy increased due to the reform, with the lowering of public resources at the profit of private funding, notably in the independent production of thermal energy versus hydroelectric energy. The results have been mixed due to the breakdown of public versus private partnerships in many countries, with the disengagement of the State. Independent power production has raised problems of sustainability and environmental issues, as well as social problems, price control, as well as accessibility in rural settings. 7 refs., 1 fig

  9. 24 CFR 401.401 - Consolidated Restructuring Plans.

    Science.gov (United States)

    2010-04-01

    ... PROGRAM (MARK-TO-MARKET) Restructuring Plan § 401.401 Consolidated Restructuring Plans. A PAE may request HUD to approve a Consolidated Restructuring Plan that presents an overall strategy for more than one... resources, HUD will not approve any Consolidated Restructuring Plans that have a detrimental effect on...

  10. Social Effects of Organisational Restructuring on Employee ...

    African Journals Online (AJOL)

    First Lady

    turn affects workforce loyalty, productivity and morale either positively or negatively ... (CEC, 2008; Brand and Wilson, 2000; Luthan and Sommer, 1999; and .... The review of literature presented an overview of organisational restructuring.

  11. Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway

    International Nuclear Information System (INIS)

    Campos, Dijon Henrique Salomé de; Leopoldo, André Soares; Lima-Leopoldo, Ana Paula; Nascimento, André Ferreira do; Oliveira-Junior, Silvio Assis de; Silva, Danielle Cristina Tomaz da; Sugizaki, Mario Mateus; Padovani, Carlos Roberto; Cicogna, Antonio Carlos

    2014-01-01

    Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced glucose leads to cardiac dysfunction in obesity. 30-day-old male Wistar rats were fed standard and hypercaloric diet for 30 weeks. Cardiac function and morphology were assessed. In this paper was viewed the general characteristics and comorbities associated to obesity. The structure cardiac was determined by weights of the heart and left ventricle (LV). Myocardial function was evaluated by studying isolated papillary muscles from the LV, under the baseline condition and after inotropic and lusitropic maneuvers: myocardial stiffness; postrest contraction; increase in extracellular Ca2+ concentration; change in heart rate and inhibitor of glycolytic pathway. Compared with control group, the obese rats had increased body fat and co-morbities associated with obesity. Functional assessment after blocking iodoacetate shows no difference in the linear regression of DT, however, the RT showed a statistically significant difference in behavior between the control and the obese group, most notable being the slope in group C. The energy imbalance on obesity did not cause cardiac dysfunction. On the contrary, the prioritization of fatty acids utilization provides protection to cardiac muscle during the inhibition of glycolysis, suggesting that this pathway is fewer used by obese cardiac muscle

  12. Tributyltin-induced apoptosis requires glycolytic adenosine trisphosphate production.

    Science.gov (United States)

    Stridh, H; Fava, E; Single, B; Nicotera, P; Orrenius, S; Leist, M

    1999-10-01

    The toxicity of tributyltin chloride (TBT) involves Ca(2+) overload, cytoskeletal damage, and mitochondrial failure leading to cell death by apoptosis or necrosis. Here, we examined whether the intracellular ATP level modulates the mode of cell death after exposure to TBT. When Jurkat cells were energized by the mitochondrial substrate, pyruvate, low concentrations of TBT (1-2 microM) triggered an immediate depletion of intracellular ATP followed by necrotic death. When ATP levels were maintained by the addition of glucose, the mode of cell death was typically apoptotic. Glycolytic ATP production was required for apoptosis at two distinct steps. First, maintenance of adequate ATP levels accelerated the decrease of mitochondrial membrane potential, and the release of the intermembrane proteins adenylate kinase and cytochrome c from mitochondria. A possible role of the adenine nucleotide exchanger in this first ATP-dependent step is suggested by experiments performed with the specific inhibitor, bongkrekic acid. This substance delayed cytochrome c release in a manner similar to that caused by ATP depletion. Second, caspase activation following cytochrome c release was only observed in ATP-containing cells. Bcl-2 had only a minor effect on TBT-triggered caspase activation or cell death. We conclude that intracellular ATP concentrations control the mode of cell death in TBT-treated Jurkat cells at both the mitochondrial and caspase activation levels.

  13. Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway

    Energy Technology Data Exchange (ETDEWEB)

    Campos, Dijon Henrique Salomé de, E-mail: dijoncampos@gmail.com [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Leopoldo, André Soares; Lima-Leopoldo, Ana Paula [Departamento de Esportes - Centro de Educação Física e Desportos da Universidade Federal do Espírito Santo (UFES), Vitória, ES (Brazil); Nascimento, André Ferreira do [Instituto de Ciências da Saúde da Universidade Federal do Mato Grosso (UFMT), Sinop, MT (Brazil); Oliveira-Junior, Silvio Assis de [Escola de Fisioterapia da Universidade Federal do Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil); Silva, Danielle Cristina Tomaz da [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Sugizaki, Mario Mateus [Instituto de Ciências da Saúde da Universidade Federal do Mato Grosso (UFMT), Sinop, MT (Brazil); Padovani, Carlos Roberto [Departamento de Bioestatística, Instituto de Ciências Biológicas da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Cicogna, Antonio Carlos, E-mail: dijoncampos@gmail.com [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil)

    2014-10-15

    Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced glucose leads to cardiac dysfunction in obesity. 30-day-old male Wistar rats were fed standard and hypercaloric diet for 30 weeks. Cardiac function and morphology were assessed. In this paper was viewed the general characteristics and comorbities associated to obesity. The structure cardiac was determined by weights of the heart and left ventricle (LV). Myocardial function was evaluated by studying isolated papillary muscles from the LV, under the baseline condition and after inotropic and lusitropic maneuvers: myocardial stiffness; postrest contraction; increase in extracellular Ca2+ concentration; change in heart rate and inhibitor of glycolytic pathway. Compared with control group, the obese rats had increased body fat and co-morbities associated with obesity. Functional assessment after blocking iodoacetate shows no difference in the linear regression of DT, however, the RT showed a statistically significant difference in behavior between the control and the obese group, most notable being the slope in group C. The energy imbalance on obesity did not cause cardiac dysfunction. On the contrary, the prioritization of fatty acids utilization provides protection to cardiac muscle during the inhibition of glycolysis, suggesting that this pathway is fewer used by obese cardiac muscle.

  14. Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway

    Directory of Open Access Journals (Sweden)

    Dijon Henrique Salomé de Campos

    2014-10-01

    Full Text Available Background: Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Objective: Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced glucose leads to cardiac dysfunction in obesity. Methods: 30-day-old male Wistar rats were fed standard and hypercaloric diet for 30 weeks. Cardiac function and morphology were assessed. In this paper was viewed the general characteristics and comorbities associated to obesity. The structure cardiac was determined by weights of the heart and left ventricle (LV. Myocardial function was evaluated by studying isolated papillary muscles from the LV, under the baseline condition and after inotropic and lusitropic maneuvers: myocardial stiffness; postrest contraction; increase in extracellular Ca2+ concentration; change in heart rate and inhibitor of glycolytic pathway. Results: Compared with control group, the obese rats had increased body fat and co-morbities associated with obesity. Functional assessment after blocking iodoacetate shows no difference in the linear regression of DT, however, the RT showed a statistically significant difference in behavior between the control and the obese group, most notable being the slope in group C. Conclusion: The energy imbalance on obesity did not cause cardiac dysfunction. On the contrary, the prioritization of fatty acids utilization provides protection to cardiac muscle during the inhibition of glycolysis, suggesting that this pathway is fewer used by obese cardiac muscle.

  15. Similar temperature dependencies of glycolytic enzymes : An evolutionary adaptation to temperature dynamics?

    NARCIS (Netherlands)

    Cruz, L.A.B.; Hebly, M.; Duong, G.H.; Wahl, S.A.; Pronk, J.T.; Heijnen, J.J.; Daran-Lapujade, P.; Van Gulik, W.M.

    2012-01-01

    Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in

  16. Annual report on contractor work force restructuring, fiscal year 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    This report summarizes work force restructuring and community transition activities at all sites. It outlines work force restructuring activity for FY 1997, changing separation patterns, cost savings and separation costs, program assessment, activities to mitigate restructuring impacts, community transition activities, status of displaced workers, lessons learned, and emerging issues in worker and community transition. Work force restructuring and community transition activities for defense nuclear sites are summarized, as are work force restructuring activities at non-defense sites.

  17. Restructuring Vocational Special Needs Education through Interdisciplinary Team Effort: Local Motion in the Pacific Basin.

    Science.gov (United States)

    Smith, Garnett J.; Stodden, Robert A.

    1994-01-01

    The Restructuring through Interdisciplinary Team Effort project focuses on changing the culture and structure of vocational special needs education in the Pacific Basin. Its three dimensions are cognitive core (best practices, outcome-focused design, strategic planning); team network of stakeholders; and systemic renewal (school-to-work…

  18. Evidence for loss of a partial flagellar glycolytic pathway during trypanosomatid evolution.

    Directory of Open Access Journals (Sweden)

    Robert W B Brown

    Full Text Available Classically viewed as a cytosolic pathway, glycolysis is increasingly recognized as a metabolic pathway exhibiting surprisingly wide-ranging variations in compartmentalization within eukaryotic cells. Trypanosomatid parasites provide an extreme view of glycolytic enzyme compartmentalization as several glycolytic enzymes are found exclusively in peroxisomes. Here, we characterize Trypanosoma brucei flagellar proteins resembling glyceraldehyde-3-phosphate dehydrogenase (GAPDH and phosphoglycerate kinase (PGK: we show the latter associates with the axoneme and the former is a novel paraflagellar rod component. The paraflagellar rod is an essential extra-axonemal structure in trypanosomes and related protists, providing a platform into which metabolic activities can be built. Yet, bioinformatics interrogation and structural modelling indicate neither the trypanosome PGK-like nor the GAPDH-like protein is catalytically active. Orthologs are present in a free-living ancestor of the trypanosomatids, Bodo saltans: the PGK-like protein from B. saltans also lacks key catalytic residues, but its GAPDH-like protein is predicted to be catalytically competent. We discuss the likelihood that the trypanosome GAPDH-like and PGK-like proteins constitute molecular evidence for evolutionary loss of a flagellar glycolytic pathway, either as a consequence of niche adaptation or the re-localization of glycolytic enzymes to peroxisomes and the extensive changes to glycolytic flux regulation that accompanied this re-localization. Evidence indicating loss of localized ATP provision via glycolytic enzymes therefore provides a novel contribution to an emerging theme of hidden diversity with respect to compartmentalization of the ubiquitous glycolytic pathway in eukaryotes. A possibility that trypanosome GAPDH-like protein additionally represents a degenerate example of a moonlighting protein is also discussed.

  19. Transcriptional responses to glucose at different glycolytic rates in Saccharomyces cerevisiae.

    Science.gov (United States)

    Elbing, Karin; Ståhlberg, Anders; Hohmann, Stefan; Gustafsson, Lena

    2004-12-01

    The addition of glucose to Saccharomyces cerevisiae cells causes reprogramming of gene expression. Glucose is sensed by membrane receptors as well as (so far elusive) intracellular sensing mechanisms. The availability of four yeast strains that display different hexose uptake capacities allowed us to study glucose-induced effects at different glycolytic rates. Rapid glucose responses were observed in all strains able to take up glucose, consistent with intracellular sensing. The degree of long-term responses, however, clearly correlated with the glycolytic rate: glucose-stimulated expression of genes encoding enzymes of the lower part of glycolysis showed an almost linear correlation with the glycolytic rate, while expression levels of genes encoding gluconeogenic enzymes and invertase (SUC2) showed an inverse correlation. Glucose control of SUC2 expression is mediated by the Snf1-Mig1 pathway. Mig1 dephosphorylation upon glucose addition is known to lead to repression of target genes. Mig1 was initially dephosphorylated upon glucose addition in all strains able to take up glucose, but remained dephosphorylated only at high glycolytic rates. Remarkably, transient Mig1-dephosphorylation was accompanied by the repression of SUC2 expression at high glycolytic rates, but stimulated SUC2 expression at low glycolytic rates. This suggests that Mig1-mediated repression can be overruled by factors mediating induction via a low glucose signal. At low and moderate glycolytic rates, Mig1 was partly dephosphorylated both in the presence of phosphorylated, active Snf1, and unphosphorylated, inactive Snf1, indicating that Mig1 was actively phosphorylated and dephosphorylated simultaneously, suggesting independent control of both processes. Taken together, it appears that glucose addition affects the expression of SUC2 as well as Mig1 activity by both Snf1-dependent and -independent mechanisms that can now be dissected and resolved as early and late/sustained responses.

  20. Restructuring still rampant in U.S

    International Nuclear Information System (INIS)

    Anon.

    1992-01-01

    This paper reports that restructuring and response to recession is far from over in the petroleum industry. Unocal Corp, disclosed a major reorganization of its operating business units, part of a company wide restructuring program first unveiled in April. In addition: Mobil Corp, made an $800 million cut to $4 billion in its 1992 capital and exploration budget. Amoco Corp, the it plans to take about $800 million in after tax charges against second quarter earnings mainly to cover the costs of writeoffs, restructuring, and workforce reductions, all part of a realignment of business operations. The company expects before tax savings of more than $600 million/year due to sweeping elimination of operating costs. The full effect of the savings will be felt by yearend 1993, with much of the savings being effected by yearend 1992

  1. The restructuring of the Ontario electricity market

    International Nuclear Information System (INIS)

    Doucet, J.A.

    1999-01-01

    A summary of the current status of the deregulation of the electricity market in Ontario was presented. To follow global deregulation trends, the Ontario Government has embarked on a considerable restructuring of the Ontario electricity market. The monopoly position of Ontario Hydro has been removed by restructuring the provincial utility into two separate companies, GENCO and SERVCO, which will be responsible for the generation and transmission and distribution of electricity, respectively. Other mechanisms put in place to favour a free and competitive market for electricity in the province, such as the arrival on the market of other electricity producers, and the establishment of the independent market operator, are also discussed. 2 tabs

  2. Managing relationships between electric power industry restructuring and grid reliability

    International Nuclear Information System (INIS)

    Thomas, R.J.

    2005-01-01

    The electricity system is a critical infrastructure, and its continued and reliable functioning is essential to the nation's economy and well-being. However, the inter-dependency of electricity networks is not completely understood. The economic impact of outages was discussed in this white paper. It was suggested that moving to a restructured environment has degraded the reliability of the bulk system. New institutional arrangements and approaches to information management are needed. It was suggested that reliability practices caused the 2003 blackout, and not technical failures. Uncertainties in the restructured market were discussed, as well as incentives to maintain system adequacy. Examples of deregulation in other countries were presented. Organizational complexities were reviewed, including the Federal Energy Regulatory Commission's (FERC) requirements and the new layers of complexity that have been added to the decision-making process in the light of restructuring. Planning and connectivity issues were reviewed. The need for design standards in power grid control centres was discussed. Difficulties in collecting data from different control centres were considered. Issues concerning the lack of investment in research and development were discussed, with particular reference to the urgent need for coordinated research programs. The looming manpower crisis in the electric power industry was also discussed. Recommendations included ensuring that the transmission system can support a market structure; building a national reliability centre; solving the manpower crisis; and testing market designs before deploying them. It was concluded that good engineering design principles, including experimental economic testing, should be required of any new electricity market design before authorizing its use. 31 refs., 1 tab., 6 figs

  3. Nonlinear correlations in the hydrophobicity and average flexibility along the glycolytic enzymes sequences

    International Nuclear Information System (INIS)

    Ciorsac, Alecu; Craciun, Dana; Ostafe, Vasile; Isvoran, Adriana

    2011-01-01

    Research highlights: → We focus our study on the glycolytic enzymes. → We reveal correlation of hydrophobicity and flexibility along their chains. → We also reveal fractal aspects of the glycolytic enzymes structures and surfaces. → The glycolytic enzyme sequences are not random. → Creation of fractal structures requires the operation of nonlinear dynamics. - Abstract: Nonlinear methods widely used for time series analysis were applied to glycolytic enzyme sequences to derive information concerning the correlation of hydrophobicity and average flexibility along their chains. The 20 sequences of different types of the 10 human glycolytic enzymes were considered as spatial series and were analyzed by spectral analysis, detrended fluctuations analysis and Hurst coefficient calculation. The results agreed that there are both short range and long range correlations of hydrophobicity and average flexibility within investigated sequences, the short range correlations being stronger and indicating that local interactions are the most important for the protein folding. This correlation is also reflected by the fractal nature of the structures of investigated proteins.

  4. Nonlinear correlations in the hydrophobicity and average flexibility along the glycolytic enzymes sequences

    Energy Technology Data Exchange (ETDEWEB)

    Ciorsac, Alecu, E-mail: aleciorsac@yahoo.co [Politehnica University of Timisoara, Department of Physical Education and Sport, 2 P-ta Victoriei, 300006, Timisoara (Romania); Craciun, Dana, E-mail: craciundana@gmail.co [Teacher Training Department, West University of Timisoara, 4 Boulevard V. Pirvan, Timisoara, 300223 (Romania); Ostafe, Vasile, E-mail: vostafe@cbg.uvt.r [Department of Chemistry, West University of Timisoara, 16 Pestallozi, 300115, Timisoara (Romania); Laboratory of Advanced Researches in Environmental Protection, Nicholas Georgescu-Roegen Interdisciplinary Research and Formation Platform, 4 Oituz, Timisoara, 300086 (Romania); Isvoran, Adriana, E-mail: aisvoran@cbg.uvt.r [Department of Chemistry, West University of Timisoara, 16 Pestallozi, 300115, Timisoara (Romania); Laboratory of Advanced Researches in Environmental Protection, Nicholas Georgescu-Roegen Interdisciplinary Research and Formation Platform, 4 Oituz, Timisoara, 300086 (Romania)

    2011-04-15

    Research highlights: lights: We focus our study on the glycolytic enzymes. We reveal correlation of hydrophobicity and flexibility along their chains. We also reveal fractal aspects of the glycolytic enzymes structures and surfaces. The glycolytic enzyme sequences are not random. Creation of fractal structures requires the operation of nonlinear dynamics. - Abstract: Nonlinear methods widely used for time series analysis were applied to glycolytic enzyme sequences to derive information concerning the correlation of hydrophobicity and average flexibility along their chains. The 20 sequences of different types of the 10 human glycolytic enzymes were considered as spatial series and were analyzed by spectral analysis, detrended fluctuations analysis and Hurst coefficient calculation. The results agreed that there are both short range and long range correlations of hydrophobicity and average flexibility within investigated sequences, the short range correlations being stronger and indicating that local interactions are the most important for the protein folding. This correlation is also reflected by the fractal nature of the structures of investigated proteins.

  5. A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin.

    Science.gov (United States)

    Araiza-Olivera, Daniela; Chiquete-Felix, Natalia; Rosas-Lemus, Mónica; Sampedro, José G; Peña, Antonio; Mujica, Adela; Uribe-Carvajal, Salvador

    2013-08-01

    In the Saccharomyces cerevisiae glycolytic pathway, 11 enzymes catalyze the stepwise conversion of glucose to two molecules of ethanol plus two CO₂ molecules. In the highly crowded cytoplasm, this pathway would be very inefficient if it were dependent on substrate/enzyme diffusion. Therefore, the existence of a multi-enzymatic glycolytic complex has been suggested. This complex probably uses the cytoskeleton to stabilize the interaction of the various enzymes. Here, the role of filamentous actin (F-actin) in stabilization of a putative glycolytic metabolon is reported. Experiments were performed in isolated enzyme/actin mixtures, cytoplasmic extracts and permeabilized yeast cells. Polymerization of actin was promoted using phalloidin or inhibited using cytochalasin D or latrunculin. The polymeric filamentous F-actin, but not the monomeric globular G-actin, stabilized both the interaction of isolated glycolytic pathway enzyme mixtures and the whole fermentation pathway, leading to higher fermentation activity. The associated complexes were resistant against inhibition as a result of viscosity (promoted by the disaccharide trehalose) or inactivation (using specific enzyme antibodies). In S. cerevisiae, a glycolytic metabolon appear to assemble in association with F-actin. In this complex, fermentation activity is enhanced and enzymes are partially protected against inhibition by trehalose or by antibodies. © 2013 FEBS.

  6. 77 FR 14979 - Transportation Conformity Rule Restructuring Amendments

    Science.gov (United States)

    2012-03-14

    ... Transportation Conformity Rule Restructuring Amendments AGENCY: Environmental Protection Agency (EPA). ACTION: Final rule. SUMMARY: EPA is amending the transportation conformity rule to finalize provisions that were proposed on August 13, 2010. These amendments restructure several sections of the transportation conformity...

  7. Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells.

    Science.gov (United States)

    Chen, Jing; Hu, Xun; Cui, Jingjie

    2018-05-01

    Glycolysis is the most important source of energy for the production of anabolic building blocks in cancer cells. Therefore, glycolytic enzymes are regarded as potential targets for cancer treatment. Previously, naphthaquinones, including shikonin, vitamin K 3 and vitamin K 5 , have been proven to decrease the rate of glycolysis in cancer cells, which is partly due to suppressed pyruvate kinase activity. In the present study, enzymatic assays were performed using MCF-7 cell lysate in order to screen the profile of glycolytic enzymes in cancer cells inhibited by shikonin, vitamin K 3 and vitamin K 5 , in addition to pyruvate kinase. Results revealed that hexokinase, phosphofructokinase-1, fructose bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase produced in the process of glycolysis were inhibited by shikonin, vitamin K 3 and vitamin K 5 . The results indicated that shikonin, vitamin K 3 and vitamin K 5 are chemical inhibitors of glycolytic enzymes in cancer cells and have potential uses in translational medical applications.

  8. Urban restructuring and deregulations in Latin America

    NARCIS (Netherlands)

    Carmona, M.

    1992-01-01

    With this work we continue the debate on the trends of urban restructuring in Latin America. In fact due to the large economic crisis of the 80-ies, the continent has decreased is living standards to that of 12 years ago. The productive potential has increased in 1,3% in circumstances that in the

  9. Restructured site characterization program at Yucca Mountain

    International Nuclear Information System (INIS)

    Dyer, J.R.; Vawter, R.G.

    1995-01-01

    During 1994 and the early part of 1995, the US Department of Energy's Yucca Mountain Site Characterization Office (YMSCO) and its parent organization, the Office of Civilian Radioactive Waste Management (OCRWM) underwent a significant restructuring. Senior Department officials provided the leadership to reorient the management, technical, programmatic, and public interaction approach to the US High Level Radioactive Waste Disposal Program. The restructuring involved reorganizing the federal staff, conducting meaningful strategic planning, improving the management system, rationalizing contractor responsibilities, focusing upon major products, and increasing stakeholder involvement. The restructured program has prioritized technical and scientific activities toward meeting major regulatory milestones in a timely and cost-effective manner. This approach has raised concern among elements of technical, scientific, and oversight bodies that suitability and licensing decisions could be made without obtaining sufficient technical information for this first-of-its-kind endeavor. Other organizations, such as congressional committees, industrial groups, and rate payers believe characterization goals can be met in a timely manner and within the limitation of available funds. To balance these contrasting views in its decision making process, OCRWM management has made a special effort to communicate its strategy to oversight bodies, the scientific community and other stakeholders and to use external independent peer review as a key means of demonstrating scientific credibility. Site characterization of Yucca Mountain in Nevada is one of the key elements of the restructured program

  10. Education and Political Restructure in Nigeria

    Science.gov (United States)

    Ayeni, Matthew Adedeji; Adeleye, Joseph Olusola

    2013-01-01

    To say that education is a potent factor in any political restructuring is an indisputable fact. For a meaningful political development to take place in any nation, especially like Nigeria. The place of education is never in doubt to influence positively those in the position of authority to ascertain what are needed to put in place for effective…

  11. Dynamic Restructuring Of Problems In Artificial Intelligence

    Science.gov (United States)

    Schwuttke, Ursula M.

    1992-01-01

    "Dynamic tradeoff evaluation" (DTE) denotes proposed method and procedure for restructuring problem-solving strategies in artificial intelligence to satisfy need for timely responses to changing conditions. Detects situations in which optimal problem-solving strategies cannot be pursued because of real-time constraints, and effects tradeoffs among nonoptimal strategies in such way to minimize adverse effects upon performance of system.

  12. Viewing Reading Recovery as a Restructuring Phenomenon

    Science.gov (United States)

    Rinehart, James S.; Short, Paula Myrick

    2010-01-01

    This study investigated components of Reading Recovery that relate to a restructuring paradigm. Specifically, Reading Recovery was analyzed as a way to redesign teachers' work, empower teachers, and affect the core technology of teaching. Data were collected by a survey that consisted of open-ended questions and of categorical response items.…

  13. Redefining the Principalship in Restructuring Schools.

    Science.gov (United States)

    Murphy, Joseph

    1994-01-01

    Principals in restructuring schools are working in an increasingly turbulent policy environment that adds expectations but deletes little from their traditional roles. Two tasks form the basis of newly defined power relationships--delegating responsibilities and developing collaborative decision-making processes. Leading from the center means…

  14. Restructure Staff Development for Systemic Change

    Science.gov (United States)

    Kelly, Thomas F.

    2012-01-01

    This paper presents a systems approach based on the work of W. Edwards Deming to system wide, high impact staff development. Deming has pointed out the significance of structure in systems. By restructuring the process of staff development we can bring about cost effective improvement of the whole system. We can improve student achievement while…

  15. Restructuring Schools by Applying Deming's Management Theories.

    Science.gov (United States)

    Melvin, Charles A., III

    1991-01-01

    Four school districts adopted a school restructuring project using Deming's business management method. Deming offered alternative views of organizations based on psychology, systems, perceptual framework, and causes of variance. He listed 14 points for quality improvement. Evaluation indicated that key staff members willingly engaged in…

  16. Restructuring the energy industry: A financial perspective

    International Nuclear Information System (INIS)

    Abrams, W.A.

    1995-01-01

    This paper present eight tables summarizing financial aspects of energy industry restructuring. Historical, current, and future business characteristics of energy industries are outlined. Projections of industry characteristics are listed for the next five years and for the 21st century. Future independent power procedures related to financial aspects are also outlined. 8 tabs

  17. Restructuring and performance in India's electricity sector

    Science.gov (United States)

    Panda, Arun Kumar

    Restructuring and privatization, used as major tools in electricity sector reform, are often viewed as part of the same process and the terms used interchangeably. Although related, they represent quite different dimensions of change and reform. Privatization is the result of change in the management/ownership. Restructuring, on the other hand, refers to changes in structure such as the unbundling of vertically integrated utilities, and the introduction of competition. Most studies attempt to assess the impact of privatization of the electric utilities on their tariff structure, performance and efficiency. They have not tried to estimate the effect of restructuring on the performance of the unbundled utilities. Using panel data on the state electricity boards and the thermal power plants, and employing variance-component fixed effects and random effects models, this study examines the effects of restructuring and ownership on the performance of India's electricity sector. We also study the effects of absolute majority of political parties on performance. The study also uses a cross-country-comparison-framework to compare the electricity sector reforms of India with those of Chile, Hungary and Norway. Results show that restructuring has significantly positive effects on such performance indicators as plant availability, plant load factor, forced outage, average tariff collection, and sales revenue as a ratio of cost. With regard to labor efficiency indicators, we find mixed results. Restructuring also appears to entail reduction in the extent of cross-subsidization. However, the cost of supply seems to be unaffected by restructuring. Absolute majority of the party in government shows adverse effects on costs, sales revenue as a ratio of cost, and labor efficiency. The effects of ownership are somewhat mixed, with state ownership (as opposed to federal or private) indicating adverse effects on plant performance. Interestingly, after controlling for location

  18. The first step in restructuring the US electric industry

    Energy Technology Data Exchange (ETDEWEB)

    Barnett, A.H. [American University of Sharjah (United Arab Emirates); Reutter, K.A. [Nathan Associates, Auburn (United States); Thompson, H. [Auburn University (United States). Comer Hall

    2005-03-01

    The electric industry lobbied for its first step in restructuring, from local franchises to regulated state monopolies during the early 1900s. The present paper examines this restructuring with an empirical model that includes the introduction of natural gas and steam turbine generators. The restructuring resulted in fewer firms, higher costs, and higher prices, consistent with increased monopoly power and captured regulators. (author)

  19. The first step in restructuring the US electric industry

    International Nuclear Information System (INIS)

    Barnett, A.H.; Reutter, K.A.; Thompson, H.

    2005-01-01

    The electric industry lobbied for its first step in restructuring, from local franchises to regulated state monopolies during the early 1900s. The present paper examines this restructuring with an empirical model that includes the introduction of natural gas and steam turbine generators. The restructuring resulted in fewer firms, higher costs, and higher prices, consistent with increased monopoly power and captured regulators. (author)

  20. 7 CFR 766.112 - Additional security for restructured loans.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Additional security for restructured loans. 766.112..., DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS DIRECT LOAN SERVICING-SPECIAL Loan Servicing Programs § 766.112 Additional security for restructured loans. (a) If the borrower is delinquent prior to restructuring, the...

  1. Assessing The Impact Of Railway Restructuring In Nigeria | Ibe ...

    African Journals Online (AJOL)

    The study presents railway restructuring as a solution option to railway problems in Nigeria. It aims at ascertaining the efficiency of the critical input factors of Nigerian Railway corporation (NRC) while comparing it with that of the restructured developed and best practices railways to establish the basis for restructuring.

  2. On the functional vs. lexical nature of restructuring heads: evidence for a fine-grained classification of restructuring modals

    Directory of Open Access Journals (Sweden)

    Irene Balza

    2012-05-01

    Full Text Available This paper examines in detail the thematic and syntactic properties of a set of (non-restructuring structures in Germanic, Romance and Basque. Based on the comparison of these structures, I propose a fine-grained classification of (non-restructuring constructions that include Functional Restructuring, Semi-Lexical Restructuring, Lexical Restructuring and Non-Restructuring constructions. The four types involve complements of different underlying structures (VPs, vPs or even as large as NegP/TPs. The results of the analysis argue against Cinque’s (2005 hypothesis that all restructuring verbs are exclusively functional, and contradicts Wurmbrand’s (1999 claim that, within the mixed class of restructuring verbs, modals must be raising verbs.

  3. Restructuring local distribution services: Possibilities and limitations

    Energy Technology Data Exchange (ETDEWEB)

    Duann, D.J.

    1994-08-01

    The restructuring of local distribution services is now the focus of the natural gas industry. It is the last major step in the ``reconstitution`` of the natural gas industry and a critical clement in realizing the full benefits of regulatory and market reforms that already have taken place in the wellhead and interstate markets. It could also be the most important regulatory initiative for most end-use customers because they are affected directly by the costs and reliability of distribution services. Several factors contribute to the current emphasis on distribution service restructuring. They include the unbundling and restructuring of upstream markets, a realization of the limitations of supply-side options (such as gas procurement oversight), and the increased diversity and volatility of gas demand facing local distribution companies. Local distribution service is not one but a series of activities that start with commodity gas procurement and extend to transportation, load balancing, storage, and metering and billing of services provided. There are also considerable differences in the economies of scale and scope associated with these various activities. Thus, a mixture of supply arrangements (such as a competitive market or a monopoly) is required for the most efficient delivery of local distribution services. A distinction must be made between the supply of commodity gas and the provision of a bundled distribution service. This distinction and identification of the best supply arrangements for various distribution service components are the most critical factors in developing appropriate restructuring policies. For most state public utility commissions the criteria for service restructuring should include pursuing the economies of scale and scope in gas distribution, differentiating and matching gas service reliability and quality with customer requirements, and controlling costs associated with the search, negotiation, and contracting of gas services.

  4. The glycolytic flux in Escherichia coli is controlled by the demand for ATP

    DEFF Research Database (Denmark)

    Købmann, Brian Jensen; Westerhoff, H.V.; Snoep, J.L.

    2002-01-01

    additional ATP hydrolysis, we increased the flux through glycolysis to 1.7 times that of the wild-type flux. The results demonstrate why attempts in the past to increase the glycolytic flux through overexpression of glycolytic enzymes have been unsuccessful: the majority of flux control (> 75%) resides...... not inside but outside the pathway, i.e., with the enzymes that hydrolyze ATP. These data further allowed us to answer the question of whether catabolic or anabolic reactions control the growth of E. coli. We show that the majority of the control of growth rate resides in the anabolic reactions, i...

  5. Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases

    DEFF Research Database (Denmark)

    Olsen, Lars Folke; Andersen, Ann Zahle; Lunding, Anita

    2009-01-01

    ,3'-diethyloxacarbocyanine iodide. The responses of glycolytic and membrane potential oscillations to a number of inhibitors of glycolysis, mitochondrial electron flow, and mitochondrial and plasma membrane H(+)-ATPase were investigated. Furthermore, the glycolytic flux was determined as the rate of production of ethanol....../ATP antiporter and the mitochondrial F(0)F(1)-ATPase. The results further suggest that ATP hydrolysis, through the action of the mitochondrial F(0)F(1)-ATPase and plasma membrane H(+)-ATPase, are important in regulating these oscillations. We conclude that it is glycolysis that drives the oscillations...

  6. Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor

    DEFF Research Database (Denmark)

    Lehning, Christina Eva; Siedler, Solvej; Ellabaan, Mostafa M Hashim

    2017-01-01

    validated the glycolytic flux dependency of the biosensor in a range of different carbon sources in six different E. coli strains and during mevalonate production. Furthermore, we studied the flux-altering effects of genome-wide single gene knock-outs in E. coli in a multiplex FlowSeq experiment. From...... a library consisting of 2126 knock-out mutants, we identified 3 mutants with high-flux and 95 mutants with low-flux phenotypes that did not have severe growth defects. This approach can improve our understanding of glycolytic flux regulation improving metabolic models and engineering efforts....

  7. Preslaughter handling effects on pork quality and glycolytic potential in two muscles differing in fiber type composition

    NARCIS (Netherlands)

    Hambrecht, E.; Eissen, J.J.; Newman, D.J.; Smits, C.H.M.; Verstegen, M.W.A.; Hartog, den L.A.

    2005-01-01

    The objective of the present experiment was to investigate the effects of transportation, lairage, and preslaughter stressor treatment on glycolytic potential and pork quality of the glycolytic longissimus and the oxidative supraspinatus (SSP) or serratus ventralis (SV) muscles. In a 2 x 2 x 2

  8. The extent to which ATP demand controls the glycolytic flux depends strongly on the organism and conditions for growth

    DEFF Research Database (Denmark)

    Købmann, Brian Jensen; Westerhoff, H.V.; Snoep, J.L.

    2002-01-01

    Using molecular genetics we have introduced uncoupled ATPase activity in two different bacterial species, Escherichia coli and Lactococcus lactis, and determined the elasticities of the growth rate and glycolytic flux towards the intracellular [ATP]/[ADP] ratio. During balanced growth in batch...... cultures of E. coli the ATP demand was found to have almost full control on the glycolytic flux (FCC=0.96) and the flux could be stimulated by 70%. In contrast to this, in L. lactis the control by ATP demand on the glycolytic flux was close to zero. However, when we used non-growing cells of L. lactis...... (which have a low glycolytic flux) the ATP demand had a high flux control and the flux could be stimulated more than two fold. We suggest that the extent to which ATP demand controls the glycolytic flux depends on how much excess capacity of glycolysis is present in the cells....

  9. Primordial oscillations in life: Direct observation of glycolytic oscillations in individual HeLa cervical cancer cells

    Science.gov (United States)

    Amemiya, Takashi; Shibata, Kenichi; Itoh, Yoshihiro; Itoh, Kiminori; Watanabe, Masatoshi; Yamaguchi, Tomohiko

    2017-10-01

    We report the first direct observation of glycolytic oscillations in HeLa cervical cancer cells, which we regard as primordial oscillations preserved in living cells. HeLa cells starved of glucose or both glucose and serum exhibited glycolytic oscillations in nicotinamide adenine dinucleotide (NADH), exhibiting asynchronous intercellular behaviors. Also found were spatially homogeneous and inhomogeneous intracellular NADH oscillations in the individual cells. Our results demonstrate that starved HeLa cells may be induced to exhibit glycolytic oscillations by either high-uptake of glucose or the enhancement of a glycolytic pathway (Crabtree effect or the Warburg effect), or both. Their asynchronous collective behaviors in the oscillations were probably due to a weak intercellular coupling. Elucidation of the relationship between the mechanism of glycolytic dynamics in cancer cells and their pathophysiological characteristics remains a challenge in future.

  10. Synchronization of glycolytic oscillations in a yeast cell population

    DEFF Research Database (Denmark)

    Dano, S.; Hynne, F.; De Monte, Silvia

    2001-01-01

    The mechanism of active phase synchronization in a suspension of oscillatory yeast cells has remained a puzzle for almost half a century. The difficulty of the problem stems from the fact that the synchronization phenomenon involves the entire metabolic network of glycolysis and fermentation, and...

  11. Impact of Autoantibodies against Glycolytic Enzymes on Pathogenicity of Autoimmune Retinopathy and Other Autoimmune Disorders

    Directory of Open Access Journals (Sweden)

    Grazyna Adamus

    2017-04-01

    Full Text Available Autoantibodies (AAbs against glycolytic enzymes: aldolase, α-enolase, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate kinase are prevalent in sera of patients with blinding retinal diseases, such as paraneoplastic [cancer-associated retinopathy (CAR] and non-paraneoplastic autoimmune retinopathies, as well as in many other autoimmune diseases. CAR is a degenerative disease of the retina characterized by sudden vision loss in patients with cancer and serum anti-retinal AAbs. In this review, we discuss the widespread serum presence of anti-glycolytic enzyme AAbs and their significance in autoimmune diseases. There are multiple mechanisms responsible for antibody generation, including the innate anti-microbial response, anti-tumor response, or autoimmune response against released self-antigens from damaged, inflamed tissue. AAbs against enolase, GADPH, and aldolase exist in a single patient in elevated titers, suggesting their participation in pathogenicity. The lack of restriction of AAbs to one disease may be related to an increased expression of glycolytic enzymes in various metabolically active tissues that triggers an autoimmune response and generation of AAbs with the same specificity in several chronic and autoimmune conditions. In CAR, the importance of serum anti-glycolytic enzyme AAbs had been previously dismissed, but the retina may be without pathological consequence until a failure of the blood–retinal barrier function, which would then allow pathogenic AAbs access to their retinal targets, ultimately leading to damaging effects.

  12. Switch between life history strategies due to changes in glycolytic enzyme gene dosage in Saccharomyces cerevisiae.

    Science.gov (United States)

    Wang, Shaoxiao; Spor, Aymé; Nidelet, Thibault; Montalent, Pierre; Dillmann, Christine; de Vienne, Dominique; Sicard, Delphine

    2011-01-01

    Adaptation is the process whereby a population or species becomes better fitted to its habitat through modifications of various life history traits which can be positively or negatively correlated. The molecular factors underlying these covariations remain to be elucidated. Using Saccharomyces cerevisiae as a model system, we have investigated the effects on life history traits of varying the dosage of genes involved in the transformation of resources into energy. Changing gene dosage for each of three glycolytic enzyme genes (hexokinase 2, phosphoglucose isomerase, and fructose-1,6-bisphosphate aldolase) resulted in variation in enzyme activities, glucose consumption rate, and life history traits (growth rate, carrying capacity, and cell size). However, the range of effects depended on which enzyme was expressed differently. Most interestingly, these changes revealed a genetic trade-off between carrying capacity and cell size, supporting the discovery of two extreme life history strategies already described in yeast populations: the "ants," which have lower glycolytic gene dosage, take up glucose slowly, and have a small cell size but reach a high carrying capacity, and the "grasshoppers," which have higher glycolytic gene dosage, consume glucose more rapidly, and allocate it to a larger cell size but reach a lower carrying capacity. These results demonstrate antagonist pleiotropy for glycolytic genes and show that altered dosage of a single gene drives a switch between two life history strategies in yeast.

  13. Status of power sector restructuring in Lithuania

    International Nuclear Information System (INIS)

    Bachiauskas, A.

    1999-01-01

    Lithuanian electricity sector comprises joint stock company Lietuvos Energija, which is responsible for supplying the country with electric energy, the Ignalina NPP state enterprise, which generates more then three fourth of the entire electricity of Lithuania, CHPs run By municipalities of Kaunas and Vilnius, and a few power plants, owned by industrial enterprises, as well as private small hydro power plants. Joint stock company Lietuvos Energija is a vertically integrated monopoly, which has inherited drawbacks inherent in planned - administrational economy and is therefore now being restructured into a modern and effective structure capable of operating within market economy conditions. The main goals of restructuring would meet electricity economy requirements: clearly un bundle electricity generation costs from electricity transmission and distribution costs; create internal and external electricity market conditions by gradual transition to wholesale and retail electricity trade; create conditions for third party access; create favourable conditions for competition; provide a possibility to integrate with Baltic electricity market in progress

  14. Restructuring of uranium industry in Romania

    International Nuclear Information System (INIS)

    Comsa, O; Meglea, C; Paraschiva, V.; Popescu, C.; Gheorghe, A.; Meglea, S.

    2001-01-01

    A project is described which aims at evaluation of uranium industry and assessment of the technical options for lowering production costs for uranium ore and, implicitly, for nuclear fuel. The main objective is defining the optimal technical and organisational solutions leading to a functional restructuring of this activity, as well as, to implementing modern techniques, technologies and procedures, and, at the same time to lowering the economical and environmental costs. This project is performed in co-operation with IAEA in the frame of TC-ROM/3/003, 'Restructuring the Uranium Mining Industry' project. The following results of carrying out this project are expected: refurbishment of processes and technological procedures, re-dimensioning uranium industry in accordance with the dimensions of nuclear power programme, reducing the environmental impact and lowering the uranium cost

  15. Restructuring in the electricity industry: [proceedings

    International Nuclear Information System (INIS)

    1995-01-01

    Restructuring is the pivotal concept in today's marketplace, implying new organizational structures for a more effective and efficient delivery of goods and services to an increasingly discriminating community of consumers. The conference was held in Toronto in February 1995, to examine the forces driving the change in organizational structures and methods for delivering services, and to examine the most effective ways to bring about change specifically in the electrical industry. Problems of definition, open access, the role of provincial governments, the perspectives of individual utilities, competitive electric power systems in the UK and elsewhere, impact of industry restructuring on municipal utilities, and implications for public policy were debated at length. Regulatory control, steps in implementing a new structure, and ways of balancing regulatory and commercial constraints were also discussed

  16. Familiarisation: Restructuring Layouts with Visual Learning Models

    OpenAIRE

    Todi, Kashyap; Jokinen, Jussi; Luyten, Kris; Oulasvirta, Antti

    2018-01-01

    In domains where users are exposed to large variations in visuo-spatial features among designs, they often spend excess time searching for common elements (features) in familiar locations. This paper contributes computational approaches to restructuring layouts such that features on a new, unvisited interface can be found quicker. We explore four concepts of familiarisation, inspired by the human visual system (HVS), to automatically generate a familiar design for each user. Given a histor...

  17. Lexical restructuring in the absence of literacy.

    Science.gov (United States)

    Venturaa, Paulo; Kolinsky, Régine; Fernandesa, Sandra; Queridoa, Luís; Morais, José

    2007-11-01

    Vocabulary growth was suggested to prompt the implementation of increasingly finer-grained lexical representations of spoken words in children (e.g., [Metsala, J. L., & Walley, A. C. (1998). Spoken vocabulary growth and the segmental restructuring of lexical representations: precursors to phonemic awareness and early reading ability. In J. L. Metsala & L. C. Ehri (Eds.), Word recognition in beginning literacy (pp. 89-120). Hillsdale, NJ: Erlbaum.]). Although literacy was not explicitly mentioned in this lexical restructuring hypothesis, the process of learning to read and spell might also have a significant impact on the specification of lexical representations (e.g., [Carroll, J. M., & Snowling, M. J. (2001). The effects of global similarity between stimuli on children's judgments of rime and alliteration. Applied Psycholinguistics, 22, 327-342.]; [Goswami, U. (2000). Phonological representations, reading development and dyslexia: Towards a cross-linguistic theoretical framework. Dyslexia, 6, 133-151.]). This is what we checked in the present study. We manipulated word frequency and neighborhood density in a gating task (Experiment 1) and a word-identification-in-noise task (Experiment 2) presented to Portuguese literate and illiterate adults. Ex-illiterates were also tested in Experiment 2 in order to disentangle the effects of vocabulary size and literacy. There was an interaction between word frequency and neighborhood density, which was similar in the three groups. These did not differ even for the words that are supposed to undergo lexical restructuring the latest (low frequency words from sparse neighborhoods). Thus, segmental lexical representations seem to develop independently of literacy. While segmental restructuring is not affected by literacy, it constrains the development of phoneme awareness as shown by the fact that, in Experiment 3, neighborhood density modulated the phoneme deletion performance of both illiterates and ex-illiterates.

  18. Services under siege--the restructuring imperative.

    Science.gov (United States)

    Roach, S S

    1991-01-01

    Recent job losses in the U.S. service sector do not reflect a temporary recession. Those jobs are gone, the result of a massive restructuring of the sector that is just getting under way. The explanation for the restructuring is quite simple. Until recently, services have been shielded by regulation and confronted by few foreign competitors. They have allowed their white-collar payrolls to become bloated, their investment in information technology to outstrip the paybacks, and their productivity to stagnate. Now competition is heating up and exposing these inefficiencies. Just as intense competition forced the restructuring of Smokestack America in the 1980s, deregulation and foreign direct investment are shaking out service companies that cannot confront their shortcomings. The need for sweeping change in the service sector may come as a great shock to Americans who saw services as the means to continued economic prosperity. But there is a painful irony at work: job creation, the very thing proponents use to demonstrate the U.S. service sector's strength, is in fact a symptom of the sector's chronic neglect of economic efficiency. It is precisely that neglect that makes the service sector vulnerable as the race for market share intensifies and new players shift the terms of competition. Services must respond to the new competitive environment, but not by indiscriminate cost cutting. Instead, they should balance financial discipline with a comprehensive and immediate reexamination of strategy.

  19. The capital structure of banks and practice of bank restructuring

    OpenAIRE

    Dübel, Achim

    2013-01-01

    This study presents an empirical analysis of capital and liability management in eight cases of bank restructurings and resolutions from eight different European countries. It can be read as a companion piece to an earlier study by the author covering the specific bank restructuring programs of Greece, Spain and Cyprus during 2012/13. The study portrays for each case the timelines between the initial credit event and the (last) restructuring. It proceeds to discuss the capital and liability m...

  20. Alternative models for restructuring Ontario's electric sector

    International Nuclear Information System (INIS)

    Bright, D.; Salaff, S.

    1996-01-01

    The future of Ontario Hydro and the provincial electrical sector was discussed. Various models proposed for restructuring Ontario's electric sector were described and views of some of the stake holders were presented, among them the views of AMPCO, MEA, the Ontario Chamber of Commerce, IPPSO, Ontario Hydro Management, Energy Probe and the Power Workers' Union. In general, most stake holders were in favour of privatization to some degree except for the Power Workers' Union which was unalterably opposed to privatization, claiming that it would lead to quantum increases in electricity rates. 2 figs

  1. Former Soviet refineries face modernization, restructuring

    International Nuclear Information System (INIS)

    Anon.

    1993-01-01

    A massive modernization and restructuring program is under way in the refining sector of Russia and other former Soviet republics. Economic reforms and resulting economic dislocation following the collapse of the Soviet Union has left refineries in the region grappling with a steep decline and changes in product demand. At the same time, rising oil prices and an aging, dilapidated infrastructure promise a massive shakeout. Even as many refineries in the former Soviet Union (FSU) face possible closure because they are running at a fraction of capacity, a host of revamps, expansions, and grass roots refineries are planned or under way. The paper discusses plans

  2. Optimization Specifications for CUDA Code Restructuring Tool

    KAUST Repository

    Khan, Ayaz

    2017-03-13

    In this work we have developed a restructuring software tool (RT-CUDA) following the proposed optimization specifications to bridge the gap between high-level languages and the machine dependent CUDA environment. RT-CUDA takes a C program and convert it into an optimized CUDA kernel with user directives in a configuration file for guiding the compiler. RTCUDA also allows transparent invocation of the most optimized external math libraries like cuSparse and cuBLAS enabling efficient design of linear algebra solvers. We expect RT-CUDA to be needed by many KSA industries dealing with science and engineering simulation on massively parallel computers like NVIDIA GPUs.

  3. Restructuring in Turkish power generation and transmission

    International Nuclear Information System (INIS)

    Dilli, B.

    2001-01-01

    Restructuring of power sector is an evolutionary process and it is in its early days. As the reform proceeds and the market evolve and become more efficient, a number of unresolved issues shall be overcomed through re-regulations. So, the most important point is the smooth transition to the liberalized markets. The solution is dependent upon the assimilation, acceptation and supporting of the reform by the parties concerned. Market participants' acceptance and actions will be the key element for the success of the new market. (author)

  4. Serdemetan antagonizes the Mdm2-HIF1α axis leading to decreased levels of glycolytic enzymes.

    Directory of Open Access Journals (Sweden)

    Jason A Lehman

    Full Text Available Serdemetan (JNJ-26854165, an antagonist to Mdm2, was anticipated to promote the activation of p53. While regulation of p53 by Mdm2 is important, Mdm2 also regulates numerous proteins involved in diverse cellular functions. We investigated if Serdemetan would alter the Mdm2-HIF1α axis and affect cell survival in human glioblastoma cells independently of p53. Treatment of cells with Serdemetan under hypoxia resulted in a decrease in HIF1α levels. HIF1α downstream targets, VEGF and the glycolytic enzymes (enolase, phosphoglycerate kinase1/2, and glucose transporter 1, were all decreased in response to Serdemetan. The involvement of Mdm2 in regulating gene expression of glycolytic enzymes raises the possibility of side effects associated with therapeutically targeting Mdm2.

  5. Exploiting Unique Structural and Functional Properties of Malarial Glycolytic Enzymes for Antimalarial Drug Development

    Directory of Open Access Journals (Sweden)

    Asrar Alam

    2014-01-01

    Full Text Available Metabolic enzymes have been known to carry out a variety of functions besides their normal housekeeping roles known as “moonlighting functions.” These functionalities arise from structural changes induced by posttranslational modifications and/or binding of interacting proteins. Glycolysis is the sole source of energy generation for malaria parasite Plasmodium falciparum, hence a potential pathway for therapeutic intervention. Crystal structures of several P. falciparum glycolytic enzymes have been solved, revealing that they exhibit unique structural differences from the respective host enzymes, which could be exploited for their selective targeting. In addition, these enzymes carry out many parasite-specific functions, which could be of potential interest to control parasite development and transmission. This review focuses on the moonlighting functions of P. falciparum glycolytic enzymes and unique structural differences and functional features of the parasite enzymes, which could be exploited for therapeutic and transmission blocking interventions against malaria.

  6. The Structural and Functional Coordination of Glycolytic Enzymes in Muscle: Evidence of a Metabolon?

    Directory of Open Access Journals (Sweden)

    Lynda Menard

    2014-09-01

    Full Text Available Metabolism sustains life through enzyme-catalyzed chemical reactions within the cells of all organisms. The coupling of catalytic function to the structural organization of enzymes contributes to the kinetic optimization important to tissue-specific and whole-body function. This coupling is of paramount importance in the role that muscle plays in the success of Animalia. The structure and function of glycolytic enzyme complexes in anaerobic metabolism have long been regarded as a major regulatory element necessary for muscle activity and whole-body homeostasis. While the details of this complex remain to be elucidated through in vivo studies, this review will touch on recent studies that suggest the existence of such a complex and its structure. A potential model for glycolytic complexes and related subcomplexes is introduced.

  7. Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria

    DEFF Research Database (Denmark)

    Sánchez-Pascuala, Alberto; Nikel, Pablo I.; de Lorenzo, Víctor

    2018-01-01

    the potential applications of such a portable tool for targeted pathway engineering, in the present protocol we describe how the genes encoding all the enzymes of the linear EMP route have been individually recruited from the genome of E. coli K-12, edited in silico to remove their endogenous regulatory signals......The Embden-Meyerhof-Parnas (EMP) pathway is widely accepted to be the biochemical standard of glucose catabolism. The well-characterized glycolytic route of Escherichia coli, based on the EMP catabolism, is an example of an intricate pathway in terms of genomic organization of the genes involved...... and patterns of gene expression and regulation. This intrinsic genetic and metabolic complexity renders it difficult to engineer glycolytic activities and transfer them onto other microbial cell factories, thus limiting the biotechnological potential of bacterial hosts that lack the route. Taking into account...

  8. Acceleration of the glycolytic flux by steroid receptor coactivator-2 is essential for endometrial decidualization.

    Directory of Open Access Journals (Sweden)

    Ramakrishna Kommagani

    2013-10-01

    Full Text Available Early embryo miscarriage is linked to inadequate endometrial decidualization, a cellular transformation process that enables deep blastocyst invasion into the maternal compartment. Although much of the cellular events that underpin endometrial stromal cell (ESC decidualization are well recognized, the individual gene(s and molecular pathways that drive the initiation and progression of this process remain elusive. Using a genetic mouse model and a primary human ESC culture model, we demonstrate that steroid receptor coactivator-2 (SRC-2 is indispensable for rapid steroid hormone-dependent proliferation of ESCs, a critical cell-division step which precedes ESC terminal differentiation into decidual cells. We reveal that SRC-2 is required for increasing the glycolytic flux in human ESCs, which enables rapid proliferation to occur during the early stages of the decidualization program. Specifically, SRC-2 increases the glycolytic flux through induction of 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 (PFKFB3, a major rate-limiting glycolytic enzyme. Similarly, acute treatment of mice with a small molecule inhibitor of PFKFB3 significantly suppressed the ability of these animals to exhibit an endometrial decidual response. Together, these data strongly support a conserved mechanism of action by which SRC-2 accelerates the glycolytic flux through PFKFB3 induction to provide the necessary bioenergy and biomass to meet the demands of a high proliferation rate observed in ESCs prior to their differentiation into decidual cells. Because deregulation of endometrial SRC-2 expression has been associated with common gynecological disorders of reproductive-age women, this signaling pathway, involving SRC-2 and PFKFB3, promises to offer new clinical approaches in the diagnosis and/or treatment of a non-receptive uterus in patients presenting idiopathic infertility, recurrent early pregnancy loss, or increased time to pregnancy.

  9. Programmed mitophagy is essential for the glycolytic switch during cell differentiation.

    Science.gov (United States)

    Esteban-Martínez, Lorena; Sierra-Filardi, Elena; McGreal, Rebecca S; Salazar-Roa, María; Mariño, Guillermo; Seco, Esther; Durand, Sylvère; Enot, David; Graña, Osvaldo; Malumbres, Marcos; Cvekl, Ales; Cuervo, Ana María; Kroemer, Guido; Boya, Patricia

    2017-06-14

    Retinal ganglion cells (RGCs) are the sole projecting neurons of the retina and their axons form the optic nerve. Here, we show that embryogenesis-associated mouse RGC differentiation depends on mitophagy, the programmed autophagic clearance of mitochondria. The elimination of mitochondria during RGC differentiation was coupled to a metabolic shift with increased lactate production and elevated expression of glycolytic enzymes at the mRNA level. Pharmacological and genetic inhibition of either mitophagy or glycolysis consistently inhibited RGC differentiation. Local hypoxia triggered expression of the mitophagy regulator BCL2/adenovirus E1B 19-kDa-interacting protein 3-like (BNIP3L, best known as NIX) at peak RGC differentiation. Retinas from NIX-deficient mice displayed increased mitochondrial mass, reduced expression of glycolytic enzymes and decreased neuronal differentiation. Similarly, we provide evidence that NIX-dependent mitophagy contributes to mitochondrial elimination during macrophage polarization towards the proinflammatory and more glycolytic M1 phenotype, but not to M2 macrophage differentiation, which primarily relies on oxidative phosphorylation. In summary, developmentally controlled mitophagy promotes a metabolic switch towards glycolysis, which in turn contributes to cellular differentiation in several distinct developmental contexts. © 2017 The Authors.

  10. Quantitative proteomics of the tonoplast reveals a role for glycolytic enzymes in salt tolerance.

    Science.gov (United States)

    Barkla, Bronwyn J; Vera-Estrella, Rosario; Hernández-Coronado, Marcela; Pantoja, Omar

    2009-12-01

    To examine the role of the tonoplast in plant salt tolerance and identify proteins involved in the regulation of transporters for vacuolar Na(+) sequestration, we exploited a targeted quantitative proteomics approach. Two-dimensional differential in-gel electrophoresis analysis of free flow zonal electrophoresis separated tonoplast fractions from control, and salt-treated Mesembryanthemum crystallinum plants revealed the membrane association of glycolytic enzymes aldolase and enolase, along with subunits of the vacuolar H(+)-ATPase V-ATPase. Protein blot analysis confirmed coordinated salt regulation of these proteins, and chaotrope treatment indicated a strong tonoplast association. Reciprocal coimmunoprecipitation studies revealed that the glycolytic enzymes interacted with the V-ATPase subunit B VHA-B, and aldolase was shown to stimulate V-ATPase activity in vitro by increasing the affinity for ATP. To investigate a physiological role for this association, the Arabidopsis thaliana cytoplasmic enolase mutant, los2, was characterized. These plants were salt sensitive, and there was a specific reduction in enolase abundance in the tonoplast from salt-treated plants. Moreover, tonoplast isolated from mutant plants showed an impaired ability for aldolase stimulation of V-ATPase hydrolytic activity. The association of glycolytic proteins with the tonoplast may not only channel ATP to the V-ATPase, but also directly upregulate H(+)-pump activity.

  11. MCT4 Defines a Glycolytic Subtype of Pancreatic Cancer with Poor Prognosis and Unique Metabolic Dependencies

    Directory of Open Access Journals (Sweden)

    GuemHee Baek

    2014-12-01

    Full Text Available KRAS mutation, which occurs in ∼95% of pancreatic ductal adenocarcinoma (PDA, has been shown to program tumor metabolism. MCT4 is highly upregulated in a subset of PDA with a glycolytic gene expression program and poor survival. Models with high levels of MCT4 preferentially employ glycolytic metabolism. Selectively in such “addicted” models, MCT4 attenuation compromised glycolytic flux with compensatory induction of oxidative phosphorylation and scavenging of metabolites by macropinocytosis and autophagy. In spite of these adaptations, MCT4 depletion induced cell death characterized by elevated reactive oxygen species and metabolic crisis. Cell death induced by MCT4-depletion was augmented by inhibition of compensatory pathways. In xenograft models, MCT4 had a significant impact on tumor metabolism and was required for rapid tumor growth. Together, these findings illustrate the metabolic diversity of PDA described by MCT4, delineate pathways through which this lactate transporter supports cancer growth, and demonstrate that PDA can be rationally targeted based on metabolic addictions.

  12. Similar temperature dependencies of glycolytic enzymes: an evolutionary adaptation to temperature dynamics?

    Directory of Open Access Journals (Sweden)

    Cruz Luisa Ana B

    2012-12-01

    Full Text Available Abstract Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in Saccharomyces cerevisiae during temperature changes. Results Saccharomyces cerevisiae was grown under different temperature regimes and glucose availability conditions. These included glucose-excess batch cultures at different temperatures and glucose-limited chemostat cultures, subjected to fast linear temperature shifts and circadian sinoidal temperature cycles. An observed temperature-independent relation between intracellular levels of glycolytic metabolites and residual glucose concentration for all experimental conditions revealed that it is the substrate availability rather than temperature that determines intracellular metabolite profiles. This observation corresponded with predictions generated in silico with a kinetic model of yeast glycolysis, when the catalytic capacities of all glycolytic enzymes were set to share the same normalized temperature dependency. Conclusions From an evolutionary perspective, such similar temperature dependencies allow cells to adapt more rapidly to temperature changes, because they result in minimal perturbations of intracellular metabolite levels, thus circumventing the need for extensive modification of enzyme levels.

  13. Glycolytic control of vacuolar-type ATPase activity: A mechanism to regulate influenza viral infection

    Energy Technology Data Exchange (ETDEWEB)

    Kohio, Hinissan P.; Adamson, Amy L., E-mail: aladamso@uncg.edu

    2013-09-15

    As new influenza virus strains emerge, finding new mechanisms to control infection is imperative. In this study, we found that we could control influenza infection of mammalian cells by altering the level of glucose given to cells. Higher glucose concentrations induced a dose-specific increase in influenza infection. Linking influenza virus infection with glycolysis, we found that viral replication was significantly reduced after cells were treated with glycolytic inhibitors. Addition of extracellular ATP after glycolytic inhibition restored influenza infection. We also determined that higher levels of glucose promoted the assembly of the vacuolar-type ATPase within cells, and increased vacuolar-type ATPase proton-transport activity. The increase of viral infection via high glucose levels could be reversed by inhibition of the proton pump, linking glucose metabolism, vacuolar-type ATPase activity, and influenza viral infection. Taken together, we propose that altering glucose metabolism may be a potential new approach to inhibit influenza viral infection. - Highlights: • Increased glucose levels increase Influenza A viral infection of MDCK cells. • Inhibition of the glycolytic enzyme hexokinase inhibited Influenza A viral infection. • Inhibition of hexokinase induced disassembly the V-ATPase. • Disassembly of the V-ATPase and Influenza A infection was bypassed with ATP. • The state of V-ATPase assembly correlated with Influenza A infection of cells.

  14. Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria.

    Science.gov (United States)

    Sánchez-Pascuala, Alberto; Nikel, Pablo I; de Lorenzo, Víctor

    2018-01-01

    The Embden-Meyerhof-Parnas (EMP) pathway is widely accepted to be the biochemical standard of glucose catabolism. The well-characterized glycolytic route of Escherichia coli, based on the EMP catabolism, is an example of an intricate pathway in terms of genomic organization of the genes involved and patterns of gene expression and regulation. This intrinsic genetic and metabolic complexity renders it difficult to engineer glycolytic activities and transfer them onto other microbial cell factories, thus limiting the biotechnological potential of bacterial hosts that lack the route. Taking into account the potential applications of such a portable tool for targeted pathway engineering, in the present protocol we describe how the genes encoding all the enzymes of the linear EMP route have been individually recruited from the genome of E. coli K-12, edited in silico to remove their endogenous regulatory signals, and synthesized de novo following a standard (i.e., GlucoBrick) that facilitates their grouping in the form of functional modules that can be combined at the user's will. This novel genetic tool allows for the à la carte implementation or boosting of EMP pathway activities into different Gram-negative bacteria. The potential of the GlucoBrick platform is further illustrated by engineering novel glycolytic activities in the most representative members of the Pseudomonas genus (Pseudomonas putida and Pseudomonas aeruginosa).

  15. Extracellular functions of glycolytic enzymes of parasites: unpredicted use of ancient proteins.

    Science.gov (United States)

    Gómez-Arreaza, Amaranta; Acosta, Hector; Quiñones, Wilfredo; Concepción, Juan Luis; Michels, Paul A M; Avilán, Luisana

    2014-02-01

    In addition of their usual intracellular localization where they are involved in catalyzing reactions of carbohydrate and energy metabolism by glycolysis, multiple studies have shown that glycolytic enzymes of many organisms, but notably pathogens, can also be present extracellularly. In the case of parasitic protists and helminths, they can be found either secreted or attached to the surface of the parasites. At these extracellular localizations, these enzymes have been shown to perform additional, very different so-called "moonlighting" functions, such as acting as ligands for a variety of components of the host. Due to this recognition, different extracellular glycolytic enzymes participate in various important parasite-host interactions such as adherence and invasion of parasites, modulation of the host's immune and haemostatic systems, promotion of angiogenesis, and acquisition of specific nutrients by the parasites. Accordingly, extracellular glycolytic enzymes are important for the invasion of the parasites and their establishment in the host, and in determining their virulence. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Glycolytic control of vacuolar-type ATPase activity: A mechanism to regulate influenza viral infection

    International Nuclear Information System (INIS)

    Kohio, Hinissan P.; Adamson, Amy L.

    2013-01-01

    As new influenza virus strains emerge, finding new mechanisms to control infection is imperative. In this study, we found that we could control influenza infection of mammalian cells by altering the level of glucose given to cells. Higher glucose concentrations induced a dose-specific increase in influenza infection. Linking influenza virus infection with glycolysis, we found that viral replication was significantly reduced after cells were treated with glycolytic inhibitors. Addition of extracellular ATP after glycolytic inhibition restored influenza infection. We also determined that higher levels of glucose promoted the assembly of the vacuolar-type ATPase within cells, and increased vacuolar-type ATPase proton-transport activity. The increase of viral infection via high glucose levels could be reversed by inhibition of the proton pump, linking glucose metabolism, vacuolar-type ATPase activity, and influenza viral infection. Taken together, we propose that altering glucose metabolism may be a potential new approach to inhibit influenza viral infection. - Highlights: • Increased glucose levels increase Influenza A viral infection of MDCK cells. • Inhibition of the glycolytic enzyme hexokinase inhibited Influenza A viral infection. • Inhibition of hexokinase induced disassembly the V-ATPase. • Disassembly of the V-ATPase and Influenza A infection was bypassed with ATP. • The state of V-ATPase assembly correlated with Influenza A infection of cells

  17. An introduction to electric industry restructuring

    Energy Technology Data Exchange (ETDEWEB)

    Eisenberg, J.F.; Berry, L.G.

    1997-09-01

    This paper briefly describes the electric industry, its residential markets, industry structure and current trends. Its purpose is to provide Weatherization grants managers with the background necessary to assess their leveraging opportunities in an industry that is experiencing sweeping changes, commonly known as electric industry restructuring. The study describes the terrain of a changing industry topography on a national and regional basis, with some state and local information also provided. Weatherization managers and subgrantees who read this paper should be better able to understand the leveraging opportunities that are emerging now in the electricity market place. The reader will be introduced to the basics of the electric industry as it presently operates, the nature of the changes that are in the process of occurring, and the driving forces that are behind those changes. The major industry players are described by type and their interests are explored in further depth. There will also be an overview of the regulatory process as it has operated historically, as well as the changes now underway at both the state and federal levels. Finally, the paper will conclude with a description of some of the assets and opportunities available to those who may be interested in participating in the restructuring process in order to expand or protect low-income programs in their own states.

  18. Educators' Perception of Administrative Leadership throughout School Restructuring

    Science.gov (United States)

    Johnson, LeJuan

    2012-01-01

    Leaders in schools today have a crucial responsibility to employ school reform and restructure initiatives for the betterment of the student. This study sought educators' perceptions of administrative leadership throughout school restructuring. The survey design assisted in connecting educators, levels of administrator's leadership, and…

  19. Restructuring: A School-Based Plan of Action.

    Science.gov (United States)

    Rothberg, Robert A.; Bozeman, William C.

    The processes by which school leaders can institutionalize staff and organizational development, with a focus on initiating school restructuring through team development, are described. Three features are necessary for implementing school restructuring through team development: a deliberate plan for staff involvement, a positive work environment,…

  20. Implications and effectiveness of information management while restructuring an organisation

    OpenAIRE

    2008-01-01

    This research paper undertakes to outline factors of Information Management that organisations should consider when attempting a restructuring process. Conversely, valuable information and knowledge are often mislaid, overlooked or discarded to the eventual detriment of an organisation during the processes of downsizing, rightsizing, restructuring, reorganisation, reengineering, transformation or change. In the global economy organisations are always striving to keep ahead of competition and ...

  1. Assertion Training and Cognitive Restructuring With College Women.

    Science.gov (United States)

    Terrill, Marilyn J.

    The process of assertive behavior change of college women who received assertion training (AT) and cognitive restructuring was examined to assess the relative effects of different durations of exposure to cognitive restructuring. Undergraduate and graduate women students (N=27) at a state university volunteered and were screened for AT groups.…

  2. Institutional Theory in College Restructuring: Myth or Reality?

    Science.gov (United States)

    Bealing, William, Jr.; Riordan, Diane; Riordan, Michael

    2011-01-01

    In response to external pressure for change, the subject university engaged in major restructuring activities during the mid to late 1990's. As predicted by organizational theory, faculty reacted to the restructuring in order to define the conditions and methods of their work and to legitimate their professional autonomy. Although this university…

  3. An empirical examination of restructured electricity prices

    International Nuclear Information System (INIS)

    Knittel, C.R.; Roberts, M.R.

    2005-01-01

    We present an empirical analysis of restructured electricity prices. We study the distributional and temporal properties of the price process in a non-parametric framework, after which we parametrically model the price process using several common asset price specifications from the asset-pricing literature, as well as several less conventional models motivated by the peculiarities of electricity prices. The findings reveal several characteristics unique to electricity prices including several deterministic components of the price series at different frequencies. An 'inverse leverage effect' is also found, where positive shocks to the price series result in larger increases in volatility than negative shocks. We find that forecasting performance in dramatically improved when we incorporate features of electricity prices not commonly modelled in other asset prices. Our findings have implications for how empiricists model electricity prices, as well as how theorists specify models of energy pricing. (author)

  4. Ten Australian ICU nurses' perceptions of organisational restructuring.

    Science.gov (United States)

    Wynne, Rochelle

    2004-02-01

    The Australian healthcare system underwent radical reform in the 1990s as economic rationalist policies were embraced. As a result, there was significant organisational restructuring within hospitals. Traditional indicators, such as nursing absenteeism and attrition, increase during times of organisational change. Despite this, nurses' views of healthcare reform are under-represented in the literature and little is known about the impact of organisational restructuring on perceived performance. This study investigated the perceived impact of organisational restructuring on a group of intensive care unit (ICU) nurses' workplace performance. It employed a qualitative approach to collect data from a purposive sample of clinical nurses. The primary method of data collection was semi-structured interviews. Content analysis generated three categories of data. Participants identified constant pressure, inadequate communication and organisational components of restructuring within the hospital as issues that had a significant impact on their workplace performance. They perceived organisational restructuring was poorly communicated, and this resulted in an environment of constant pressure. Organisational components of restructuring included the subcategories of specialised service provision and an alternative administrative structure that had both positive and negative ramifications for performance. To date, there has been little investigation of nurses' perceptions of organisational restructure or the impact this type of change has in the clinical domain. Participants in this study believed reorganisation was detrimental to quality care delivery in intensive care, as a result of fiscal constraint, inadequate communication and pressure that influenced their workplace performance.

  5. Sodium bicarbonate ingestion increases glycolytic contribution and improves performance during simulated taekwondo combat.

    Science.gov (United States)

    Lopes-Silva, João Paulo; Da Silva Santos, Jonatas Ferreira; Artioli, Guilherme Giannini; Loturco, Irineu; Abbiss, Chris; Franchini, Emerson

    2018-04-01

    To investigate the effect of sodium bicarbonate (NaHCO 3 ) on performance and estimated energy system contribution during simulated taekwondo combat. Nine taekwondo athletes completed two experimental sessions separated by at least 48 h. Athletes consumed 300 mg/kg body mass of NaHCO 3 or placebo (CaCO 3 ) 90 min before the combat simulation (three rounds of 2 min separated by 1 min passive recovery), in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration [La - ] and rating of perceived exertion (RPE) were measured before and after each round, whereas heart rate (HR) and the estimated contribution of the oxidative (W OXI ), ATP (adenosine triphosphate)-phosphocreatine (PCr) (W PCR ), and glycolytic (W [ La - ] ) systems were calculated during the combat simulation. [La - ] increased significantly after NaHCO 3 ingestion, when compared with the placebo condition (+14%, P = 0.04, d = 3.70). NaHCO 3 ingestion resulted in greater estimated glycolytic energy contribution in the first round when compared with the placebo condition (+31%, P = 0.01, d = 3.48). Total attack time was significantly greater after NaHCO 3 when compared with placebo (+13%, P = 0.05, d = 1.15). W OXI , W PCR , VO 2 , HR and RPE were not different between conditions (P > 0.05). NaHCO 3 ingestion was able to increase the contribution of glycolytic metabolism and, therefore, improve performance during simulated taekwondo combat.

  6. Tin-Containing Silicates: Identification of a Glycolytic Pathway via 3-Deoxyglucosone

    DEFF Research Database (Denmark)

    Tolborg, Søren; Meier, Sebastian; Sádaba, I.

    2016-01-01

    a cascade of four to five sequential steps. Currently, there is a limited understanding of the competing glycolytic pathways within these systems. Here we identify dehydration of glucose to 3-deoxyglucosone as an important pathway that occurs in addition to retro-aldol reaction of hexoses when using tin......-containing silicates. It is possible to influence the relative carbon flux through these pathways by controlling the amount of alkali metal salts present in the reaction mixture. In the absence of added potassium carbonate, at least 15–30% carbon flux via 3-deoxyglucosone is observed. Addition of just a few ppm...

  7. Development of an intracellular glycolytic flux sensor for high throughput applications in E.coli

    DEFF Research Database (Denmark)

    Lehning, Christina Eva

    The aim of this PhD project was to construct, test and apply an intracellular, growth-­‐ independent and direct measureable glycolytic flux biosensor in E. coli. Studying the metabolic flux of bacterial cells is of growing interest as it is of fundamental importance to bacterial physiology as well...... to study the flux-­‐altering effects of gene knockouts in E. coli at the single cell level in a vastly parallelized and high-­‐throughput manner. After growth for several generations in rich and minimal media, 2126 gene knockouts, mainly outside of the core metabolism, could be screened. 3 gene knockouts...

  8. Industrial restructuring as an instrument for enhancing national competitiveness

    OpenAIRE

    Muradov, Shahbaz; Yuzbashieva, Gulshen

    2008-01-01

    This article considers matters of fostering national economic competitiveness in the context of industrial restructuring. The authors show the dependence of dynamic and sustainable economic and social development on the frequency and depth of structural transformations in the economy.

  9. Electricity distribution industry restructuring in South Africa: A case study

    International Nuclear Information System (INIS)

    Gaunt, C.T.

    2008-01-01

    For 20 years there has been vigorous debate about restructuring the large, developed electricity industry in South Africa, but the plans have not been implemented. The justification of the proposed restructuring illustrates the different expectations of the participants in the electricity industry. The case study reviews what has occurred against a background of some theories about organisations, and identifies six key issues. Some lessons include the need to adopt an industry structure compatible with the objectives of electricity distribution in developing countries, the unsuitability of orthodox approaches to organisations, that no industry structure appears to be significantly better than others, industry restructuring of public sector utilities has a significant political dimension, restructuring can fail even if there is support from a high level of government, and indecision and uncertainty have serious negative consequences. The experience should be helpful for other developing countries considering structural change of their electricity industry

  10. RESTRUCTURED ELECTRICITY MARKETS: Three States' Experiences in Adding Generating Capacity

    National Research Council Canada - National Science Library

    2002-01-01

    ...., restructured electricity markets by shifting from service provided through a regulated monopoly-the local electric utility-to service provided through open competition among the local utility and its competitors...

  11. The effects of union mergers and internal restructuring:

    DEFF Research Database (Denmark)

    Navrbjerg, Steen Erik; Larsen, Trine Pernille

    2016-01-01

    Trade union mergers and restructuring are often seen as strategy for union revitalisation and renewal and have been ongoing in the majority of European trade unions. This paper explores how recent union mergers and internal restructuring has affected unions service provision, union democracy...... and interest representation. This is analysed drawing on longitudinal data from two Danish shop steward surveys conducted in 1998 and 2010, comparing shop stewards in merged and non-merged unions respectively. The main results are that although it could be expected that union mergers and internal restructuring......, where union size and less so the union’s recent merger and restructuring history appears to affect shop stewards’ relations with their unions....

  12. Impacts of restructuring tabacco excise tax in the Philippines

    International Development Research Centre (IDRC) Digital Library (Canada)

    A recently approved law restructuring tobacco taxes in the. Philippines includes a steep increase in the excise tax (the tax paid by producers which ... part of Canada's International Development Research Centre. (IDRC), a Canadian Crown ...

  13. Canadian R and M restructuring to pay dividends

    International Nuclear Information System (INIS)

    Anon.

    1992-01-01

    This paper reports on massive rationalization under way in Canada's refining/marketing sector that will yield increased utilization rates, improved efficiencies, and a boost in profits. At the same time, independent marketers in Canada will bear the brunt of a shrinking market, which is occurring at a time when sharply higher environmental spending will further squeeze them. Those are the main points of an analysis by Wood Gundy Inc., Toronto. Canada's refining/marketing sector has begun a major restructuring that after about 18 months will result in a significantly smaller industry, Wood Gundy the. All major integrated companies in the country have disclosed restructuring plans that will result in closure of perhaps three or more refineries and more than 2,700 service stations. Underlying the restructuring is industry's recognition that an 11% decline in produce demand during 1990-91, stemming from restructuring of the Canadian economy and progress in energy conservation, is permanent

  14. Defense Industry Restructuring: Updated Cost and Savings Information

    National Research Council Canada - National Science Library

    1998-01-01

    ...) the restructuring costs were allowable under the Federal Acquisition Regulation and (2) a DOD contracting officer determined the business combination would result in overall reduced costs to DOD or preserve a critical defense capability...

  15. Restructuring health care through nursing and business acumen.

    Science.gov (United States)

    Goodroe, J H

    1998-03-01

    This nurse entrepreneur owns two companies that help others restructure health care processes. Utilizing knowledge from her managerial and business background, as well as clinical innovations in cardiovascular disease, set the stage for this author's successful business ventures.

  16. Restructuring EPA's Libraries: Background and Issues for Congress

    National Research Council Canada - National Science Library

    Bearden, David M; Esworthy, Robert

    2007-01-01

    ..., $2.0 million of which was attributed mainly to these closures. EPA reports that the closings are part of its efforts to restructure its libraries to respond to the increasing use of the Internet to access its collections...

  17. Transient contribution of left posterior parietal cortex to cognitive restructuring.

    Science.gov (United States)

    Sutoh, Chihiro; Matsuzawa, Daisuke; Hirano, Yoshiyuki; Yamada, Makiko; Nagaoka, Sawako; Chakraborty, Sudesna; Ishii, Daisuke; Matsuda, Shingo; Tomizawa, Haruna; Ito, Hiroshi; Tsuji, Hiroshi; Obata, Takayuki; Shimizu, Eiji

    2015-03-17

    Cognitive restructuring is a fundamental method within cognitive behavioural therapy of changing dysfunctional beliefs into flexible beliefs and learning to react appropriately to the reality of an anxiety-causing situation. To clarify the neural mechanisms of cognitive restructuring, we designed a unique task that replicated psychotherapy during a brain scan. The brain activities of healthy male participants were analysed using functional magnetic resonance imaging. During the brain scan, participants underwent Socratic questioning aimed at cognitive restructuring regarding the necessity of handwashing after using the restroom. The behavioural result indicated that the Socratic questioning effectively decreased the participants' degree of belief (DOB) that they must wash their hands. Alterations in the DOB showed a positive correlation with activity in the left posterior parietal cortex (PPC) while the subject thought about and rated own belief. The involvement of the left PPC not only in planning and decision-making but also in conceptualization may play a pivotal role in cognitive restructuring.

  18. Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS.

    Directory of Open Access Journals (Sweden)

    Chinatsu Mukai

    Full Text Available Maintaining activity of enzymes tethered to solid interfaces remains a major challenge in developing hybrid organic-inorganic devices. In nature, mammalian spermatozoa have overcome this design challenge by having glycolytic enzymes with specialized targeting domains that enable them to function while tethered to a cytoskeletal element. As a step toward designing a hybrid organic-inorganic ATP-generating system, we implemented a biomimetic site-specific immobilization strategy to tether two glycolytic enzymes representing different functional enzyme families: triose phosphoisomerase (TPI; an isomerase and glyceraldehyde 3-phosphate dehydrogenase (GAPDHS; an oxidoreductase. We then evaluated the activities of these enzymes in comparison to when they were tethered via classical carboxyl-amine crosslinking. Both enzymes show similar surface binding regardless of immobilization method. Remarkably, specific activities for both enzymes were significantly higher when tethered using the biomimetic, site-specific immobilization approach. Using this biomimetic approach, we tethered both enzymes to a single surface and demonstrated their function in series in both forward and reverse directions. Again, the activities in series were significantly higher in both directions when the enzymes were coupled using this biomimetic approach versus carboxyl-amine binding. Our results suggest that biomimetic, site-specific immobilization can provide important functional advantages over chemically specific, but non-oriented attachment, an important strategic insight given the growing interest in recapitulating entire biological pathways on hybrid organic-inorganic devices.

  19. Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS.

    Science.gov (United States)

    Mukai, Chinatsu; Gao, Lizeng; Bergkvist, Magnus; Nelson, Jacquelyn L; Hinchman, Meleana M; Travis, Alexander J

    2013-01-01

    Maintaining activity of enzymes tethered to solid interfaces remains a major challenge in developing hybrid organic-inorganic devices. In nature, mammalian spermatozoa have overcome this design challenge by having glycolytic enzymes with specialized targeting domains that enable them to function while tethered to a cytoskeletal element. As a step toward designing a hybrid organic-inorganic ATP-generating system, we implemented a biomimetic site-specific immobilization strategy to tether two glycolytic enzymes representing different functional enzyme families: triose phosphoisomerase (TPI; an isomerase) and glyceraldehyde 3-phosphate dehydrogenase (GAPDHS; an oxidoreductase). We then evaluated the activities of these enzymes in comparison to when they were tethered via classical carboxyl-amine crosslinking. Both enzymes show similar surface binding regardless of immobilization method. Remarkably, specific activities for both enzymes were significantly higher when tethered using the biomimetic, site-specific immobilization approach. Using this biomimetic approach, we tethered both enzymes to a single surface and demonstrated their function in series in both forward and reverse directions. Again, the activities in series were significantly higher in both directions when the enzymes were coupled using this biomimetic approach versus carboxyl-amine binding. Our results suggest that biomimetic, site-specific immobilization can provide important functional advantages over chemically specific, but non-oriented attachment, an important strategic insight given the growing interest in recapitulating entire biological pathways on hybrid organic-inorganic devices.

  20. MCT1-mediated transport of a toxic molecule is an effective strategy for targeting glycolytic tumors

    Science.gov (United States)

    Birsoy, Kivanc; Wang, Tim; Possemato, Richard; Yilmaz, Omer H.; Koch, Catherine E.; Chen, Walter W.; Hutchins, Amanda W.; Gultekin, Yetis; Peterson, Tim R.; Carette, Jan E.; Brummelkamp, Thijn R.; Clish, Clary B.; Sabatini, David M.

    2012-01-01

    SUMMARY There is increasing evidence that oncogenic transformation modifies the metabolic program of cells. A common alteration is the upregulation of glycolysis, and efforts to target glycolytic enzymes for anti-cancer therapy are underway. Here, we performed a genome-wide haploid genetic screen to identify resistance mechanisms to 3-bromopyruvate (3-BrPA), a drug candidate that inhibits glycolysis in a poorly understood fashion. We identified the SLC16A1 gene product, MCT1, as the main determinant of 3-BrPA sensitivity. MCT1 is necessary and sufficient for 3-BrPA uptake by cancer cells. Additionally, MCT1 mRNA levels are the best predictor of 3-BrPA sensitivity and are most elevated in glycolytic cancer cells. Lastly, forced MCT1 expression in 3-BrPA resistant cancer cells sensitizes tumor xenografts to 3-BrPA treatment in vivo. Our results identify a potential biomarker for 3-BrPA sensitivity and provide proof of concept that the selectivity of cancer-expressed transporters can be exploited for delivering toxic molecules to tumors. PMID:23202129

  1. Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus

    Science.gov (United States)

    Zhang, Yuehui; Sun, Xue; Sun, Xiaoyan; Meng, Fanci; Hu, Min; Li, Xin; Li, Wei; Wu, Xiao-Ke; Brännström, Mats; Shao, Ruijin; Billig, Håkan

    2016-01-01

    Peripheral insulin resistance and hyperandrogenism are the primary features of polycystic ovary syndrome (PCOS). However, how insulin resistance and hyperandrogenism affect uterine function and contribute to the pathogenesis of PCOS are open questions. We treated rats with insulin alone or in combination with human chorionic gonadotropin (hCG) and showed that peripheral insulin resistance and hyperandrogenism alter uterine morphology, cell phenotype, and cell function, especially in glandular epithelial cells. These defects are associated with an aberration in the PI3K/Akt signaling pathway that is used as an indicator for the onset of insulin resistance in classical metabolic tissues. Concomitantly, increased GSK3β (Ser-9) phosphorylation and decreased ERK1/2 phosphorylation in rats treated with insulin and hCG were also observed. We also profiled the expression of glucose transporter (Glut) isoform genes in the uterus under conditions of insulin resistance and/or hyperandrogenism. Finally, we determined the expression pattern of glycolytic enzymes and intermediates during insulin resistance and hyperandrogenism in the uterus. These findings suggest that the PI3K/Akt and MAPK/ERK signaling pathways play a role in the onset of uterine insulin resistance, and they also suggest that changes in specific Glut isoform expression and alterations to glycolytic metabolism contribute to the endometrial dysfunction observed in PCOS patients. PMID:27461373

  2. Role of hexose transport in control of glycolytic flux in Saccharomyces cerevisiae.

    Science.gov (United States)

    Elbing, Karin; Larsson, Christer; Bill, Roslyn M; Albers, Eva; Snoep, Jacky L; Boles, Eckhard; Hohmann, Stefan; Gustafsson, Lena

    2004-09-01

    The yeast Saccharomyces cerevisiae predominantly ferments glucose to ethanol at high external glucose concentrations, irrespective of the presence of oxygen. In contrast, at low external glucose concentrations and in the presence of oxygen, as in a glucose-limited chemostat, no ethanol is produced. The importance of the external glucose concentration suggests a central role for the affinity and maximal transport rates of yeast's glucose transporters in the control of ethanol production. Here we present a series of strains producing functional chimeras between the hexose transporters Hxt1 and Hxt7, each of which has distinct glucose transport characteristics. The strains display a range of decreasing glycolytic rates resulting in a proportional decrease in ethanol production. Using these strains, we show for the first time that at high glucose levels, the glucose uptake capacity of wild-type S. cerevisiae does not control glycolytic flux during exponential batch growth. In contrast, our chimeric Hxt transporters control the rate of glycolysis to a high degree. Strains whose glucose uptake is mediated by these chimeric transporters will undoubtedly provide a powerful tool with which to examine in detail the mechanism underlying the switch between fermentation and respiration in S. cerevisiae and will provide new tools for the control of industrial fermentations.

  3. Restructuring and privatization in energy sector

    International Nuclear Information System (INIS)

    Stojchev, D.; Pyrvanov, V.

    1994-01-01

    The ways of solving problems of the transition period to market economy are discussed. The current conditions in Bulgarian energy sector are defined taking into account different processes, stages, elements, objects. The criteria of the transition -economical. technological, organizational, social, ecological -and the problems - unemployment, requalification, privatization, contamination - are postulated. The recent experience of Bulgaria and other ex-communist countries in restructuring and privatization of the economy are considered. The scope of suitable approaches, methods, means and rates are outlined. The mechanisms of the tackled processes are analyzed by comparative investigation and management ways for impact on different levels are looked for. The possible consequences of given situation, advantages and shortcomings of different alternatives are formulated. The ways for assessment and selection of compromise solutions are proposed. An overall technology for assessment and application of different ways of transition is discussed. Their tools for business estimation of economic units, the legislative, economic and social aspects of the process are scrutiny observed. Some problems of a real example of application of proposed assessment are discussed. Conclusions about methodology and efficiency of different alternatives are made. 2 refs

  4. The restructuring of the Russian oil industry

    International Nuclear Information System (INIS)

    Price, J.

    1994-01-01

    The Russian Oil and Gas Corporation, Rosneftegas, was set up in October 1990 to be the central coordinating body of the Russian oil industry. Rosneftegas decided to seek expert advice from international advisers on the restructuring of the industry in the transition to a market economy. The advisers put forward a framework for change based on eight internal and external factors. On the internal, industry, side they are: private ownership, market structures, competition and demarcation of responsibilities. On the external, policy side they are: oil and gas legislation; taxation; contracts; and pricing. A list of investment policy and industry structure objectives was defined on the basis of the framework. Much progress has been made and many of the detailed conclusions of the advisers' report have been implemented. Many difficult issues remain to be dealt with, however. These include considerable tension between the Russian Federal government and the regional governments, between production associations and their local governments, and over the domestic price of oil and oil products. (UK)

  5. International oil market: instability and restructuring

    Energy Technology Data Exchange (ETDEWEB)

    Ayoub, A

    1988-12-01

    The three phenomena which today dominate the international oil market are: 1. the downward price trend since 1981 and the uncertainty about medium and long term price evolution; 2. chronic price instability in the short term; 3. the trend toward new forms of vertical integration and concentration which are now stabilizing a market confronted by a weakening OPEC and free markets which are volatile. The new market restructuring the present period for the international oil sector as a transition period, with the following characteristics: 1. an evident convergence in the motivations of the major oil companies and of a number of OPEC countries, to see a certain stability restored in the oil market based on vertical integration and concentration; 2. markets cannot be stabilized by political agreements between the states, but only by reciprocal financial implications and participations between companies according to the rules of the business world; 3. the market as a whole will still continue for a certain time to be governed by the OPEC-free market pair, with alternating domination by one or the other according to the economic situation. Nevertheless, the longer the market continues to be unstable, the more the trend toward concentration and integration will intensify.

  6. Surface Restructuring of Hybrid Perovskite Crystals

    KAUST Repository

    Banavoth, Murali

    2016-11-07

    Hybrid perovskite crystals have emerged as an important class of semiconductors because of their remarkable performance in optoelectronics devices. The interface structure and chemistry of these crystals are key determinants of the device\\'s performance. Unfortunately, little is known about the intrinsic properties of the surfaces of perovskite materials because extrinsic effects, such as complex microstructures, processing conditions, and hydration under ambient conditions, are thought to cause resistive losses and high leakage current in solar cells. We reveal the intrinsic structural and optoelectronic properties of both pristinely cleaved and aged surfaces of single crystals. We identify surface restructuring on the aged surfaces (visualized on the atomic-scale by scanning tunneling microscopy) that lead to compositional and optical bandgap changes as well as degradation of carrier dynamics, photocurrent, and solar cell device performance. The insights reported herein clarify the key variables involved in the performance of perovskite-based solar cells and fabrication of high-quality surface single crystals, thus paving the way toward their future exploitation in highly efficient solar cells.

  7. Modular load flow for restructured power systems

    CERN Document Server

    Hariharan, M V; Gupta, Pragati P

    2016-01-01

    In the subject of power systems, authors felt that a re-look is necessary at some conventional methods of analysis. In this book, the authors have subjected the time-honoured load flow to a close scrutiny. Authors have discovered and discussed a new load flow procedure – Modular Load Flow. Modular Load Flow explores use of power – a scalar – as source for electrical circuits which are conventionally analysed by means of phasors – the ac voltages or currents. The method embeds Kirchhoff’s circuit laws as topological property into its scalar equations and results in a unique wonderland where phase angles do not exist! Generators are shown to have their own worlds which can be superimposed to obtain the state of the composite power system. The treatment is useful in restructured power systems where stakeholders and the system operators may desire to know individual generator contributions in line flows and line losses for commercial reasons. Solution in Modular Load Flow consists of explicit expression...

  8. Banking Restructuring Techniques in the Economical Crisis Context

    Directory of Open Access Journals (Sweden)

    Vasile Dedu

    2009-11-01

    Full Text Available Banking restructuring regained leading place in commercial banking activities following ongoing crisis. Many debtors are facing serious trouble in normal debt repayment due to increasing number and volume of non performing loans in banks’ portfolios. In order to deal with these issues and to secure existing exposures banks use restructuring techniques like payment rescheduling, debt-equity swap and write-offs to ease debtors debts and to help viable businesses to successfully survive recession.

  9. The effects of corporate restructuring on hospital policymaking.

    Science.gov (United States)

    Alexander, J A; Morlock, L L; Gifford, B D

    1988-01-01

    Hospital corporate restructuring is the segmentation of assets or functions of the hospital into separate corporations. While these functions are almost always legally separated from the hospital, their impact on hospital policymaking may be far more direct. This study examines the effects of corporate restructuring by community hospitals on the structure, composition, and activity of hospital governing boards. In general, we expect that the policymaking function of the hospital will change to adapt to the multicorporate structure implemented under corporate restructuring, as well as the overlapping boards and diversified business responsibilities of the new corporate entity. Specifically, we hypothesize that the hospital board under corporate restructuring will conform more to the "corporate" model found in the business/industrial sector and less to the "philanthropic" model common to most community hospitals to date. Analysis of survey data from 1,037 hospitals undergoing corporate restructuring from 1979-1985 and a comparison group of 1,883 noncorporately restructured hospitals suggests general support for this hypothesis. Implications for health care governance and research are discussed. PMID:3384671

  10. The effects of corporate restructuring on hospital policymaking.

    Science.gov (United States)

    Alexander, J A; Morlock, L L; Gifford, B D

    1988-06-01

    Hospital corporate restructuring is the segmentation of assets or functions of the hospital into separate corporations. While these functions are almost always legally separated from the hospital, their impact on hospital policymaking may be far more direct. This study examines the effects of corporate restructuring by community hospitals on the structure, composition, and activity of hospital governing boards. In general, we expect that the policymaking function of the hospital will change to adapt to the multicorporate structure implemented under corporate restructuring, as well as the overlapping boards and diversified business responsibilities of the new corporate entity. Specifically, we hypothesize that the hospital board under corporate restructuring will conform more to the "corporate" model found in the business/industrial sector and less to the "philanthropic" model common to most community hospitals to date. Analysis of survey data from 1,037 hospitals undergoing corporate restructuring from 1979-1985 and a comparison group of 1,883 noncorporately restructured hospitals suggests general support for this hypothesis. Implications for health care governance and research are discussed.

  11. Elaboration and acceptability of restructured hams added with jabuticaba skin

    Directory of Open Access Journals (Sweden)

    Ana Paula de Carvalho ALVES

    Full Text Available Abstract Jabuticaba skin flour (JSF, rich in antioxidants and fibre, was used in order to develop four restructured hams in different combinations with meat: 0% control – without the addition of JSF, with the addition of 0.5% JSF, 1.0% JSF and 1.5% JSF, meeting, therefore, the growing demand for meat products with functional appeal. Physicochemical characterizations and sensory analysis were performed, evaluating the impact of information on possible health benefits and the effect on storage. Restructured hams with JSF had higher contents of phenolic compounds; greater weight loss; a darker shade; texture profile with smaller parameters of stiffness, cohesiveness, adhesiveness, flexibility and chewiness (except the restructured ham with 0.5% JSF. Phenolic compounds, pH values, colour and TBARs did not differ with storage time, but were statistically different among JSF concentrations. Panellists preferred the restructured ham without JSF, however, the analysis of variance for overall aspect did not show a significant difference between the restructured ham “control” and those added with 0.5% and 1.0% JSF, after informing the panellists that they would possibly bring health benefits. It is concluded that it is possible to prepare and store restructured hams with JSF, at concentrations of 0.5% and 1.0% with good acceptance.

  12. The potential for electricity market restructuring in Quebec

    International Nuclear Information System (INIS)

    Clark, C.R.; Leach, A.

    2007-01-01

    Throughout the world, electricity market liberalization has taken place or is currently underway. However, in Canada, the province of Quebec has undertaken limited restructuring measures. Considerable debate as to the potential for further market restructuring has been ongoing. This article discussed the potential for future electricity market restructuring in Quebec where competition in electricity supply has resulted in 3 categories of supply distribution, including a block of energy and power known as the heritage pool. This article considered the political economy of the abolishment if the heritage pool requirement, which caps wholesale prices. The article presented a statistical overview of Quebec's electricity market and discussed the regulatory environment. It also identified the potential for market restructuring and the establishment of a competitive wholesale market. It was concluded that Quebecers could significantly benefit from any restructuring initiative that involved the elimination of the Heritage Pool requirement if the extra rents were properly redistributed and that production sector restructuring would be difficult. 37 refs., 3 tabs., 4 figs

  13. RESTRUCTURING OF THE LARGE-SCALE SPRINKLERS

    Directory of Open Access Journals (Sweden)

    Paweł Kozaczyk

    2016-09-01

    Full Text Available One of the best ways for agriculture to become independent from shortages of precipitation is irrigation. In the seventies and eighties of the last century a number of large-scale sprinklers in Wielkopolska was built. At the end of 1970’s in the Poznan province 67 sprinklers with a total area of 6400 ha were installed. The average size of the sprinkler reached 95 ha. In 1989 there were 98 sprinklers, and the area which was armed with them was more than 10 130 ha. The study was conducted on 7 large sprinklers with the area ranging from 230 to 520 hectares in 1986÷1998. After the introduction of the market economy in the early 90’s and ownership changes in agriculture, large-scale sprinklers have gone under a significant or total devastation. Land on the State Farms of the State Agricultural Property Agency has leased or sold and the new owners used the existing sprinklers to a very small extent. This involved a change in crop structure, demand structure and an increase in operating costs. There has also been a threefold increase in electricity prices. Operation of large-scale irrigation encountered all kinds of barriers in practice and limitations of system solutions, supply difficulties, high levels of equipment failure which is not inclined to rational use of available sprinklers. An effect of a vision of the local area was to show the current status of the remaining irrigation infrastructure. The adopted scheme for the restructuring of Polish agriculture was not the best solution, causing massive destruction of assets previously invested in the sprinkler system.

  14. Restructuring the Schoolbook Provision System in Indonesia

    Directory of Open Access Journals (Sweden)

    Dedi Supriadi

    1999-03-01

    Full Text Available Schoolbooks account for between 65% and 75% of all publishing activity in Indonesia. The amount of money allocated is continually increasing. Priority is given to the primary and junior secondary school levels (6+3 years, which are compulsory. Between 1969 and 1988, the Government of Indonesia (GOI has produced some 550 million primary textbooks and library books. Up to the year 2000, the GOI has decided to allocate the total of US$ 355.2 million to produce 250 million copies of primary and junior secondary school textbooks to reach the ratio of one book for each student. In addition, around Rp 20-50 billion (US$ 10-20 million annually is spent to purchase 8-17 million copies of reading books which are aimed at stimulating the reading interest of primary school children. These books are distributed free to some 168,000 primary and 26,969 junior secondary schools throughout Indonesia. Following the massive efforts to increase book availability at schools, some innovative policies are being taken. Book evaluation standards have been improved to ensure that only high quality books are used at schools. The distribution system has been restructured to guarantee that books reach targeted schools. Consequently, the book monitoring system has had to be strengthened to examine whether or not the books really reach the schools and are used properly by students and teachers in the classrooms. In the last three years, there has also been a growing concern with multicultural issues in schoolbook provision programs. In such a culturally diverse nation as Indonesia, schoolbooks should also be culturally sensitive and be recognize the varied sociocultural backgrounds which affect students' learning.

  15. Restructuring the Water Supply at CERN

    CERN Document Server

    Nonis, M

    1999-01-01

    The CERN water network is the result of continuous extensions made to meet the different needs of the experiments and accelerators. Several studies concerning the current water consumption and the foreseen needs for the running of the new accelerators show a need to optimize the network and, where possible, reduce the consumption. Site construction works will begin in February 1999 and will continue until 2003; important modification works on the water network will only be possible during the shutdown for the dismantling of LEP. This paper will present the technical outlines and will report the status of the project. ST Division is involved in reorganizing the demineralized and primary cooling water circuits for the accelerators while Services Industriels de Genève (SIG) will be responsible for the works on pumping stations and on water networks.

  16. Local and global bifurcations at infinity in models of glycolytic oscillations

    DEFF Research Database (Denmark)

    Sturis, Jeppe; Brøns, Morten

    1997-01-01

    We investigate two models of glycolytic oscillations. Each model consists of two coupled nonlinear ordinary differential equations. Both models are found to have a saddle point at infinity and to exhibit a saddle-node bifurcation at infinity, giving rise to a second saddle and a stable node...... at infinity. Depending on model parameters, a stable limit cycle may blow up to infinite period and amplitude and disappear in the bifurcation, and after the bifurcation, the stable node at infinity then attracts all trajectories. Alternatively, the stable node at infinity may coexist with either a stable...... sink (not at infinity) or a stable limit cycle. This limit cycle may then disappear in a heteroclinic bifurcation at infinity in which the unstable manifold from one saddle at infinity joins the stable manifold of the other saddle at infinity. These results explain prior reports for one of the models...

  17. Potential role of the glycolytic oscillator in acute hypoxia in tumors

    International Nuclear Information System (INIS)

    Fru, Leonard Che; Adamson, Erin B; Campos, David D; Fain, Sean B; Song, Chihwa; Kissick, Michael W; Jacques, Steven L; Van der Kogel, Albert J; Nickel, Kwang P; Kimple, Randall J

    2015-01-01

    Tumor acute hypoxia has a dynamic component that is also, at least partially, coherent. Using blood oxygen level dependent magnetic resonance imaging, we observed coherent oscillations in hemoglobin saturation dynamics in cell line xenograft models of head and neck squamous cell carcinoma. We posit a well-established biochemical nonlinear oscillatory mechanism called the glycolytic oscillator as a potential cause of the coherent oscillations in tumors. These data suggest that metabolic changes within individual tumor cells may affect the local tumor microenvironment including oxygen availability and therefore radiosensitivity. These individual cells can synchronize the oscillations in patches of similar intermediate glucose levels. These alterations have potentially important implications for radiation therapy and are a potential target for optimizing the cancer response to radiation. (paper)

  18. Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

    Science.gov (United States)

    Thwe, Phyu M; Pelgrom, Leonard; Cooper, Rachel; Beauchamp, Saritha; Reisz, Julie A; D'Alessandro, Angelo; Everts, Bart; Amiel, Eyal

    2017-09-05

    Dendritic cell (DC) activation by Toll-like receptor (TLR) agonists causes rapid glycolytic reprogramming that is required to meet the metabolic demands of their immune activation. Recent efforts in the field have identified an important role for extracellular glucose sourcing to support DC activation. However, the contributions of intracellular glucose stores to these processes have not been well characterized. We demonstrate that DCs possess intracellular glycogen stores and that cell-intrinsic glycogen metabolism supports the early effector functions of TLR-activated DCs. Inhibition of glycogenolysis significantly attenuates TLR-mediated DC maturation and impairs their ability to initiate lymphocyte activation. We further report that DCs exhibit functional compartmentalization of glucose- and glycogen-derived carbons, where these substrates preferentially contribute to distinct metabolic pathways. This work provides novel insights into nutrient homeostasis in DCs, demonstrating that differential utilization of glycogen and glucose metabolism regulates their optimal immune function. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. uPA/uPAR system activation drives a glycolytic phenotype in melanoma cells.

    Science.gov (United States)

    Laurenzana, Anna; Chillà, Anastasia; Luciani, Cristina; Peppicelli, Silvia; Biagioni, Alessio; Bianchini, Francesca; Tenedini, Elena; Torre, Eugenio; Mocali, Alessandra; Calorini, Lido; Margheri, Francesca; Fibbi, Gabriella; Del Rosso, Mario

    2017-09-15

    In this manuscript, we show the involvement of the uPA/uPAR system in the regulation of aerobic glycolysis of melanoma cells. uPAR over-expression in human melanoma cells controls an invasive and glycolytic phenotype in normoxic conditions. uPAR down-regulation by siRNA or its uncoupling from integrins, and hence from integrin-linked tyrosine kinase receptors (IL-TKRs), by an antagonist peptide induced a striking inhibition of the PI3K/AKT/mTOR/HIF1α pathway, resulting into impairment of glucose uptake, decrease of several glycolytic enzymes and of PKM2, a checkpoint that controls metabolism of cancer cells. Further, binding of uPA to uPAR regulates expression of molecules that govern cell invasion, including extracellular matrix metallo-proteinases inducer (EMPPRIN) and enolase, a glycolytyc enzyme that also serves as a plasminogen receptor, thus providing a common denominator between tumor metabolism and phenotypic invasive features. Such effects depend on the α5β1-integrin-mediated uPAR connection with EGFR in melanoma cells with engagement of the PI3K-mTOR-HIFα pathway. HIF-1α trans-activates genes whose products mediate tumor invasion and glycolysis, thus providing the common denominator between melanoma metabolism and its invasive features. These findings unveil a unrecognized interaction between the invasion-related uPAR and IL-TKRs in the control of glycolysis and disclose a new pharmacological target (i.e., uPAR/IL-TKRs axis) for the therapy of melanoma. © 2017 UICC.

  20. Hyperthyroidism results in increased glycolytic capacity in the rat heart. A 31P-NMR study.

    Science.gov (United States)

    Seymour, A M; Eldar, H; Radda, G K

    1990-11-12

    We have investigated the metabolic adaptations that occur in the thyroxine-treated rat heart. Rats were made hyperthyroid by daily intra-peritoneal injections of thyroxine (35 micrograms/100 g body weight) over seven days. 31P-NMR investigations of isolated glucose-perfused isometric hearts showed that thyroxine treatment caused an increase in Pi (from 4.9 mumols.(g dry wt.)-1 in control hearts to 11.7 mumols.(g dry wt.)-1 in hyperthyroid hearts), a decrease in phosphocreatine (from 36.5 mumols.(g dry wt.)-1 to 21.8 mumols.(g dry wt.)-1) with no change in ATP or ADP concentrations under the same conditions of cardiac work. The unidirectional exchange flux Pi----ATP was measured by saturation transfer NMR in hyperthyroid rat hearts. This exchange (which has been shown to contain a significant glycolytic component) increased by 2.2-fold in thyroxine-treated hearts in comparison to control hearts (to 3.6 mumols.(g dry wt.)-1.s-1, from 1.6 mumols.(g dry wt.)-1.s-1). In parallel experiments, NMR analysis of extracts from hyperthyroid rat hearts showed significantly elevated levels of glucose 6-phosphate, and fructose 6-phosphate. Measurements of enzyme activities isolated from hyperthyroid and control tissue showed a 40% increase in phosphofructokinase activity. These data together with the increased concentration of Pi show that both glycolytic and glycogenolytic fluxes are increased in the hyperthyroid rat heart. This metabolic adaptation may be necessary to cope with the increased number and activity of Na+/K(+)-ATPase pumps that occur in response to thyroxine treatment.

  1. Adenosine triphosphate levels during anaphylactic histamine release in rat mast cells in vitro. Effects of glycolytic and respiratory inhibitors

    DEFF Research Database (Denmark)

    Johansen, Torben

    1979-01-01

    The adenosine triphosphate (ATP) content of rat mast cells was studied during and after anaphylactic histamine release. The almost identical time course of ATP decrease from mast cells treated with either glycolytic or respiratory inhibitors supports the view that the ATP depletion was largely re...

  2. Glycogen Shunt Activity and Glycolytic Supercompensation in Astrocytes May Be Distinctly Mediated via the Muscle Form of Glycogen Phosphorylase

    DEFF Research Database (Denmark)

    Jakobsen, Emil; Bak, Lasse K; Walls, Anne B

    2017-01-01

    Glycogen is the main storage form of glucose in the brain. In contrast with previous beliefs, brain glycogen has recently been shown to play important roles in several brain functions. A fraction of metabolized glucose molecules are being shunted through glycogen before reentering the glycolytic ...

  3. Frequent and recent retrotransposition of orthologous genes plays a role in the evolution of sperm glycolytic enzymes

    Directory of Open Access Journals (Sweden)

    de Villena Fernando

    2010-05-01

    Full Text Available Abstract Background The central metabolic pathway of glycolysis converts glucose to pyruvate, with the net production of 2 ATP and 2 NADH per glucose molecule. Each of the ten reactions in this pathway is typically catalyzed by multiple isozymes encoded by a multigene family. Several isozymes in this pathway are expressed only during spermatogenesis, and gene targeting studies indicate that they are essential for sperm function and male fertility in mouse. At least three of the novel glycolytic isozymes are encoded by retrogenes (Pgk2, Aldoart1, and Aldoart2. Their restricted expression profile suggests that retrotransposition may play a significant role in the evolution of sperm glycolytic enzymes. Results We conducted a comprehensive genomic analysis of glycolytic enzymes in the human and mouse genomes and identified several intronless copies for all enzymes in the pathway, except Pfk. Within each gene family, a single orthologous gene was typically retrotransposed frequently and independently in both species. Several retroposed sequences maintained open reading frames (ORFs and/or provided evidence of alternatively spliced exons. We analyzed expression of sequences with ORFs and Gpi1 transcript in mouse spermatogenic cells. Conclusions Our analysis detected frequent, recent, and lineage-specific retrotransposition of orthologous glycolytic enzymes in the human and mouse genomes. Retrotransposition events are associated with LINE/LTR and genomic integration is random. We found evidence for the alternative splicing of parent genes. Many retroposed sequences have maintained ORFs, suggesting a functional role for these genes.

  4. JURIDICAL AND FISCAL ASPECTS REGARDING THE RESTRUCTURING OF

    Directory of Open Access Journals (Sweden)

    DRAGOS (COMAN DORICA

    2015-08-01

    Full Text Available The purpose of this paper is the study of the juridical and fiscal aspects regarding the restructuring of the economical entities. Based on analyzing of the different materials on legislative advancements at national level and on European directives, rules and recommendations and other reference works, this study accomplished a synthesis of the main juridical aspects regarding the economical restructuring in Romania and in the European Union. The study emphasizes the importance of the knowledge of the fiscal aspects in order to draft a fiscal strategy ,which needs to be developed prior to any formal offers or detailed studying of the target company, and which reveals the characteristics, the stages and the effects of the fusion operation of an economic entity. The results of the study points out the main juridical and fiscal aspects regarding the restructuring of the trading companies, as well as the main components of a fusion operation.

  5. Designing incentive market mechanisms for improving restructured power system reliabilities

    DEFF Research Database (Denmark)

    Ding, Yi; Østergaard, Jacob; Wu, Qiuwei

    2011-01-01

    state. The reliability management of producers usually cannot be directly controlled by the system operators in a restructured power system. Producers may have no motivation to improve their reliabilities, which can result in serious system unreliability issues in the new environment. Incentive market...... mechanisms for improving the restructured power system reliabilities have been designed in this paper. In the proposed incentive mechanisms, penalty will be implemented on a producer if the failures of its generator(s) result in the variation of electricity prices. Incentive market mechanisms can motivate......In a restructured power system, the monopoly generation utility is replaced by different electricity producers. There exists extreme price volatility caused by random failures by generation or/and transmission systems. In these cases, producers' profits can be much higher than those in the normal...

  6. COGNITIVE RESTRUCTURING: ALTERNATIVE COUNSELING TECHNIQUES TO REDUCTION ACADEMIC PROCRASTINATION

    Directory of Open Access Journals (Sweden)

    Annisa Sofiana

    2016-12-01

    Full Text Available Procrastination is often experienced by almost everyone, including students who often delay to resolve any responsibility in the academic process that would decrease the individual academic achievement. cognitive restructuring is one of the cognitive techniques used in counseling in addition to cognitive behavioral techniques (behavioral and didaktif techniques. This technique has several procedures by focusing on identifying an effort and changing dysfunctional thoughts or negative self-statements into a new belief that is more rational and adaptive, which will affect more rational behavior anyway. Cognitive restructuring techniques assessed to be an alternative counseling techniques in reducing academic procrastination.

  7. Consequences of electricity restructuring on the environment: A survey

    International Nuclear Information System (INIS)

    Sevi, Benoit

    2004-01-01

    The aim of this paper is to assess theoretical consequences of restructuring electricity markets on the environment. We examine changes in potential behaviours in consumption-side as well as in supply-side. We show that restructuring and following access to competition is not neutral from an environmental standpoint. Deregulation could induce some negative externalities due to requirements in cost-efficiency. The principal result of this paper is the need of strong incentives in public policies to compensate the new short-term horizon in which energy sector's firms are evolving, particularly concerning R and D. (author)

  8. Empirical studies of regulatory restructuring and incentives

    Science.gov (United States)

    Knittel, Christopher Roland

    This dissertation examines the actions of firms when faced with regulatory restructuring. Chapter I examines the equilibrium pricing behavior of local exchange telephone companies under a variety of market structures. In particular, the pricing behavior of three services are analyzed: residential local service, business local service, and intraLATA toll service. Beginning in 1984, a variety of market structure changes have taken place in the local telecommunications industry. I analyze differences in the method of price-setting regulation and the restrictions on entry. Specifically, the relative pricing behavior under rate of return and price cap regulation is analyzed, as well as the impact of entry in the local exchange and intraLATA toll service markets. In doing so, I estimate an empirical model that accounts for the stickiness of rates in regulated industries that is based on firm and regulator decision processes in the presence of adjustment costs. I find that, faced with competitive pressures that reduce rates in one service, incumbent firm rates increase in other services, thereby reducing the benefits from competition. In addition, the findings suggest that price cap regulation leads to higher rates relative to rate-of-return regulation. Chapter 2 analyzes the pricing and investment behavior of electricity firms. Electricity and natural gas markets have traditionally been serviced by one of two market structures. In some markets, electricity and natural gas are sold by a dual-product regulated monopolist, while in other markets, electricity and natural gas are sold by separate single-product regulated monopolies. This paper analyzes the relative pricing and investment decisions of electricity firms operating in the two market structures. The unique relationship between these two products imply that the relative incentives of single and dual-product firms are likely to differ. Namely electricity and natural gas are substitutes in consumption while natural

  9. Effects of Glycolytic-Based Interval Training on Anaerobic Capacity in Soccer Players

    Directory of Open Access Journals (Sweden)

    Polczyk Michał

    2015-09-01

    Full Text Available Purpose. The aim of this study was to determine the magnitude of changes in anaerobic endurance in response to a training protocol targeting glycolytic capacity. Methods. The study involved 24 soccer players from two U-18 teams. One team served as an experimental (E group the other a control (C. Besides standard soccer practice performed by both groups, an interval training protocol was administered to the experimental group twice a week (15 sessions. One training repetition involved running a soccerspecific course. Repetition time was equal to 15 s interspersed with 45 s passive recovery. Total number of repetitions was determined by the ability to maintain target time (power in subsequent repetitions. A 5% reduction in the distance covered (m compared with the first repetition ended a set. The number of sets was based on the ability of player to maintain target time per repetition. Rest interval between sets was 15 min. Anaerobic performance was assessed before and after the 8-week protocol by the Wingate test in which arterial blood gases, blood lactate concentration, and respiratory variables on a breath-by-breath basis were measured. Results. Distance covered in group E in the first training session was 470.38 ± 77.82 m and 1182.31 ± 164.44 m in the last session. Post-intervention total work (273.63 ± 18.32 to 284.98 ± 15.76 J/kg and maximum power (13.28 ± 1.43 to 14.14 ± 1.25 W/kg significantly increased in the Wingate test. Statistically significant increases in lactate concentration (10.64 ± 1.54 and 12.72 ± 1.59 mmol/l and lower blood pH (7.21 ± 0.03 and 7.19 ± 0.02 were also observed. No significant changes in any of the above variables were observed in group C. Conclusions. Interval training develops glycolytic capacity but with large inter-individual variability.

  10. A Petiveria alliacea standardized fraction induces breast adenocarcinoma cell death by modulating glycolytic metabolism.

    Science.gov (United States)

    Hernández, John Fredy; Urueña, Claudia Patricia; Cifuentes, Maria Claudia; Sandoval, Tito Alejandro; Pombo, Luis Miguel; Castañeda, Diana; Asea, Alexzander; Fiorentino, Susana

    2014-05-14

    Folk medicine uses aqueous and alcoholic extracts from Petiveria alliacea (Phytolaccaceae) in leukemia and breast cancer treatment in the Caribbean, Central and South America. Herein, we validated the biological activity of a Petiveria alliacea fraction using a metastatic breast adenocarcinoma model (4T1). Petiveria alliacea fraction biological activity was determined estimating cell proliferation, cell colony growth capacity and apoptosis (caspase-3 activity, DNA fragmentation and mitochondrial membrane potential) in 4T1 cells. Petiveria alliacea was used at IC₅₀ concentration (29 µg/mL) and 2 dilutions below, doxorubicin at 0.27 µg/mL (positive control) and dibenzyl disulfide at 2.93 µg/mL (IC50 fraction marker compound). Proteomic estimations were analyzed by LC-MS-MS. Protein level expression was confirmed by RT-PCR. Glucose and lactate levels were measured by enzymatic assays. LD50 was established in BALB/c mice and antitumoral activity evaluated in mice transplanted with GFP-tagged 4T1 cells. Mice were treated with Petiveria alliacea fraction via I.P (182 mg/kg corresponding to 1/8 of LD₅₀ and 2 dilutions below). Petiveria alliacea fraction in vitro induces 4T1 cells apoptosis, caspase-3 activation, DNA fragmentation without mitochondria membrane depolarization, and decreases cell colony growth capacity. Also, changes in glycolytic enzymes expression cause a decrease in glucose uptake and lactate production. Fraction also promotes breast primary tumor regression in BALB/c mice transplanted with GFP-tagged 4T1 cells. A fraction of Petiveria alliacea leaves and stems induces in vitro cell death and in vivo tumor regression in a murine breast cancer model. Our results validate in partly, the traditional use of Petiveria alliacea in breast cancer treatment, revealing a new way of envisioning Petiveria alliacea biological activity. The fraction effect on the glycolytic pathway enzymes contributes to explain the antiproliferative and antitumor activities

  11. The role of commercial banks in enterprise restructuring in Central and Eastern Europe

    OpenAIRE

    Long, Millard; Rutkowska, Izabela

    1995-01-01

    Many countries in Eastern Europe assigned banks the responsibility for restructuring enterprises. Such restructuring had five components: 1) triage of enterprises into three classes -- viable, viable with debt relief, and nonviable; 2) work with management of overindebted firms on a restructuring plan before granting debt relief; 3) trigger the bankruptcy liquidation process on nonviable firms; 4) fund new investments needed as part of physical restructuring; and 5) provide corporate governan...

  12. Load frequency control of an asynchronous restructured power system

    African Journals Online (AJOL)

    This paper presents the analysis of load frequency control (LFC) of a two-area restructured power system interconnected via parallel ac/dc transmission links. Simulation results show that the limitations of PI controller can be overcome by including Fuzzy logic concept and thereby the dynamic performance can be improved ...

  13. The effect of restructuring of health care services on caesarean ...

    African Journals Online (AJOL)

    Nicky

    2005-06-24

    Jun 24, 2005 ... The effect of restructuring of health care services on caesarean section rates. ARTICLE. Medical Research Council/ University of KwaZulu-Natal, Pregnancy Hypertension Research. Unit and Department of Obstetrics and Gynaecology, Nelson R Mandela School of Health. Sciences, University of ...

  14. Cognitive Restructuring and a Collaborative Set in Couples' Work.

    Science.gov (United States)

    Huber, Charles H.; Milstein, Barbara

    1985-01-01

    Investigated effects of cognitive restructuring efforts to modify unrealistic beliefs of marital partners in 17 couples. Treatment program sought to impact proactively upon positive therapeutic expectations and relationship goals and enhanced base level of marital satisfaction. On all outcome measures, treatment group (N=9 couples) showed…

  15. Genomic restructuring in Hordeum chilense durum wheat hybrids ...

    Indian Academy of Sciences (India)

    ANDREIA DELGADO

    4John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. 5Departament of Genetics and Biotechnology, University of Tras-os-Montes and Alto ... [Delgado A., Carvalho A., Martín A. C., Martín A. and Lima-Brito J. 2017 Genomic restructuring in F1 Hordeum chilense × durum ...... Academic Press, Burlington,.

  16. The San Diego Panasonic Partnership: A Case Study in Restructuring.

    Science.gov (United States)

    Holzman, Michael; Tewel, Kenneth J.

    1992-01-01

    The Panasonic Foundation provides resources for restructuring school districts. The article examines its partnership with the San Diego City School District, highlighting four schools that demonstrate promising practices and guiding principles. It describes recent partnership work on systemic issues, noting the next steps to be taken in San Diego.…

  17. The Study of the Effects of Cognitive Restructuring Therapy on ...

    African Journals Online (AJOL)

    This paper is an extract from a larger study. The study investigated the relative effectiveness of Cognitive Restructuring (C.R) therapy in the reduction of cigarette smoking behaviour of undergraduate students from South West of Nigeria. The research followed a pre test post test control group design. sixty volunteer ...

  18. Restructuring European supply chains by implementing postponement strategies

    NARCIS (Netherlands)

    Hoek, van Remko; Vos, G.C.J.M.; Commandeur, H.R.

    1999-01-01

    More demanding customer needs in terms of quality, variety, delivery (both fast and reliable), and competitive pricing challenge an increasing number of companies to restructure their supply chains. An appealing option is to delay, or postpone, the point of product differentiation, that is deferring

  19. Upward Mobility Through Job Restructuring. Personnel Management Series No. 26.

    Science.gov (United States)

    Civil Service Commission, Washington, DC.

    The May, 1974, bulletin issued by the Civil Service Commission deals with job restructuring, the process of realigning job duties to develop technician-type or "bridge" jobs in Federal agencies, as a means to provide upward mobility for employees. Besides being highly beneficial to employees in dead end jobs at low grade levels, job restructuring…

  20. Challenges of electric power industry restructuring for fuel suppliers

    International Nuclear Information System (INIS)

    1998-09-01

    The purpose of this report is to provide an assessment of the changes in other energy industries that could occur as the result of restructuring in the electric power industry. This report is prepared for a wide audience, including Congress, Federal and State agencies, the electric power industry, and the general public. 28 figs., 25 tabs

  1. On Restructuring Roles and Relationships: A Conversation with Phil Schlechty.

    Science.gov (United States)

    Brandt, Ron

    1993-01-01

    Restructuring is changing the system of rules, roles, and relationships that govern the use of time, people, space, knowledge, and technology. The teacher's new role is inventing engaging work. The school board's role is educating the community about school conditions. The superintendent's role is influencing stakeholder decision making. Without…

  2. Imagining the Good Organization: Educational Restructuring in a Coastal Community.

    Science.gov (United States)

    Harris, Carol E.

    2002-01-01

    Describes town of Burnt Island, Newfoundland, including its need for imaginative survival; explores the nature of imaginative thought, including benefits to the individual and to society; describes threats to imaginative teaching within town's restructured school; describes characteristics common to the work of Greenfield, Greene, and Habermas…

  3. Revenue and Health Impacts of Restructuring Tobacco Excise Tax ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Revenue and Health Impacts of Restructuring Tobacco Excise Tax in the Philippines. A proposed law in the Philippines to increase the excise tax on tobacco by 215% will likely have implications for tobacco control and consumption, and public health, not just for that country but for the region. Although half of deaths due to ...

  4. Restructuring of schools as a consequence of computer use?

    NARCIS (Netherlands)

    Plomp, T.; Pelgrum, W.J.

    1993-01-01

    The central question discussed is whether the use of computers leads to the restructuring of schools or classrooms. Several authors argue that intensive use of computers must lead to new classroom patterns or new forms of schooling. Data from the international comparative study of computers in

  5. Free trade and freer petchems drive Mexican restructuring

    International Nuclear Information System (INIS)

    Wood, A.

    1992-01-01

    When Mexico first opened up its protected markets in 1987 by cutting import tariffs, it thrust the chemical industry into a phase of change. Now, with the advent of the North American Free Trade Agreement(NAFTA) and the liberalization of petrochemicals by state oil group Petroleos Mexicanos (Pemex), restructuring has moved up a gear

  6. Challenges of electric power industry restructuring for fuel suppliers

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-09-01

    The purpose of this report is to provide an assessment of the changes in other energy industries that could occur as the result of restructuring in the electric power industry. This report is prepared for a wide audience, including Congress, Federal and State agencies, the electric power industry, and the general public. 28 figs., 25 tabs.

  7. Restructuring STM (Science, Technology, and Mathematics) Education for Entrepreneurship

    Science.gov (United States)

    Ezeudu, F. O.; Ofoegbu, T. O.; Anyaegbunnam, N. J.

    2013-01-01

    This paper discussed the need to restructure STM (science, technology, and mathematics) education to reflect entrepreneurship. This is because the present STM education has not achieved its aim of making graduates self-reliant. Entrepreneurship education if introduced in the STM education will produce graduate who can effectively manage their…

  8. 48 CFR 231.205-70 - External restructuring costs.

    Science.gov (United States)

    2010-10-01

    ... subsection: (1) Business combination means a transaction whereby assets or operations of two or more... after a business combination that affect the operations of companies not previously under common ownership or control. They do not include restructuring activities occurring after a business combination...

  9. Corporate financial restructuring in Asia: implications for financial stability

    OpenAIRE

    Michael Pomerleano

    2007-01-01

    Corporate financial fragility preceding the Asian financial crisis heightened vulnerabilities. Many countries in the region undertook significant corporate financial restructuring after the crisis, with some countries bouncing back much faster than others. These sounder corporate financial practices bode well for financial stability.

  10. School Restructuring and the Dilemmas of Principals' Work.

    Science.gov (United States)

    Wildy, Helen; Louden, William

    2000-01-01

    The complexity of principals' work may be characterized according to three dilemmas: accountability, autonomy, and efficiency. Narrative vignettes of 74 Australian principals revealed that principals were fair and inclusive. When faced with restructuring dilemmas, however, they favored strong over shared leadership, efficiency over collaboration,…

  11. Business restructuring and the strategic role of 'IT'

    International Nuclear Information System (INIS)

    Lindsay, E.

    1998-01-01

    Westcoast Energy has recently undergone a major restructuring as a result of industry deregulation. As a result of the restructuring in 1997, Westcoast, producers, shippers and customers renegotiated gathering and business processes and devised a new tolling methodology known as the Negotiated Toll Settlement. This paper describes the new structure of Westcoast, the lines of business the company is engaged in, the nature of the Negotiated Toll Settlement with emphasis on the important role that information technology (IT) played in the company's business restructuring strategies. In discussing the impact of restructuring and the Negotiated Toll Settlement on information technology, attention is drawn to the enormity of the cultural change faced by employees largely unfamiliar with change. It discusses the new skills and competencies required and the complexity introduced by the need for timeliness. The difficulties of having the delivery team in Vancouver and system support and customers in Calgary and the massive changes that had to be made to refocus attention on customer and operational concerns are outlined. Among the lessons learned, cultural change was identified as perhaps the most difficult stumbling block, followed by the need for communication, quality assurance, risk assessment and project management methodologies, and recognition of the direct relationship between business and system changes. figs

  12. Restructuring, Teams, and Learning: The Case of a Clothing Company.

    Science.gov (United States)

    Brown, Tony

    1999-01-01

    Interviews with female employees of a clothing company moving to lean production and teamwork indicate that Taylorism is being reinvented in the contemporary workplace. Restructuring produces higher productivity but greater work pressures and lower wages, despite rhetoric about autonomy, job satisfaction, and workplace democracy. (Contains 57…

  13. Public-policy responsibilities in a restructured electricity industry

    Energy Technology Data Exchange (ETDEWEB)

    Tonn, B.; Hirst, E.; Bauer, D.

    1995-06-01

    In this report, we identify and define the key public-policy values, objectives, and actions that the US electricity industry currently meets. We also discuss the opportunities for meeting these objectives in a restructured industry that relies primarily on market forces rather than on government mandates. And we discuss those functions that governments might undertake, presumably because they will not be fully met by a restructured industry on its own. These discussions are based on a variety of inputs. The most important inputs came from participants in an April 1995 workshop on Public-Policy Responsibilities and Electric Industry Restructuring: Shaping the Research Agenda. Other sources of information and insights include the reviews of a draft of this report by workshop participants and others and the rapidly growing literature on electric-industry restructuring and its implications. One of the major concerns about the future of the electricity industry is the fate of numerous social and environmental programs supported by today`s electric utilities. Many people worry that a market-driven industry may not meet the public-policy objectives that electric utilities have met in the past. Examples of potentially at-risk programs include demand-side management (DSM), renewable energy, low-income weatherization, and fuel diversity. Workshop participants represented electric utilities, public utility commissions (PUCs), state energy offices, public-interest groups, other energy providers, and the research community.

  14. Accounting for change: the micropolitics of university restructuring ...

    African Journals Online (AJOL)

    This second article in a three-part series published in the South African Journal of Higher Education, describes the various ways in which "academic identity" informed the politics and shaped the outcomes of "restructuring" at the University of Durban Westville (UDW) in the late 1990s. The authors argue that while ...

  15. Decision-making regarding restructuring in multinational enterprises.

    OpenAIRE

    Ghertman M

    1986-01-01

    ILO pub. Working paper, evaluation of three case studies of decision making regarding enterprise restructuring in multinational enterprises originating in Canada, the USA and Western Europe - examines the role of management attitude, business organization, capital resources and size of enterprise in determining subsidiary enterprise creation, enterprise takeover, plant shutdown, resource allocation, labour utilization, etc. Diagrams, organigrams, references, tables.

  16. Anthropology and Openmindedness: A Restructuring of the Social Studies Curriculum.

    Science.gov (United States)

    Dynneson, Thomas L.

    The potential use of anthropology for restructuring both the general curriculum and social studies is discussed. Anthropology could work as an organizer because it is a broad based discipline and relates to the natural sciences, fine arts, language arts, and humanities, as well as to the social sciences. By the beginning of the 21st century, major…

  17. Actin restructuring during Salmonella typhimurium infection investigated by confocal and super-resolution microscopy

    Science.gov (United States)

    Han, Jason J.; Kunde, Yuliya A.; Hong-Geller, Elizabeth; Werner, James H.

    2014-01-01

    We have used super-resolution optical microscopy and confocal microscopy to visualize the cytoskeletal restructuring of HeLa cells that accompanies and enables Salmonella typhimurium internalization. Herein, we report the use of confocal microscopy to verify and explore infection conditions that would be compatible with super-resolution optical microscopy, using Alexa-488 labeled phalloidin to stain the actin cytoskeletal network. While it is well known that actin restructuring and cytoskeletal rearrangements often accompany and assist in bacterial infection, most studies have employed conventional diffraction-limited fluorescence microscopy to explore these changes. Here we show that the superior spatial resolution provided by single-molecule localization methods (such as direct stochastic optical reconstruction microscopy) enables more precise visualization of the nanoscale changes in the actin cytoskeleton that accompany bacterial infection. In particular, we found that a thin (100-nm) ring of actin often surrounds an invading bacteria 10 to 20 min postinfection, with this ring being transitory in nature. We estimate that a few hundred monofilaments of actin surround the S. typhimurium in this heretofore unreported bacterial internalization intermediate.

  18. Empirical Correlates and Expanded Interpretation of the MMPI-2-RF Restructured Clinical Scale 3 (Cynicism)

    Science.gov (United States)

    Ingram, Paul B.; Kelso, Kristy M.; McCord, David M.

    2011-01-01

    The recent release of the Minnesota Multiphasic Personality Inventory-2-Restructured Form (MMPI-2-RF) has received much attention from the clinical psychology community. Particular concerns have focused on Restructured Clinical Scale 3 (RC3; Cynicism). This article briefly reviews the major criticisms and responses regarding the restructuring of…

  19. Expanding the Evaluation Department Role in a Restructuring District: A Technical Assistance Model.

    Science.gov (United States)

    Borton, William M.

    Although San Diego City Schools is entering its fourth year of restructuring efforts, many schools are still reluctant to assume the autonomy offered to them. If school restructuring is stalling, it is because district offices have not yet restructured, research on what is known about implementing changes has been ignored, and no clear methodology…

  20. The impact of restructuring on employee well-being: a systematic review of longitudinal studies

    NARCIS (Netherlands)

    Jong, T. de; Wiezer, N.; Weerd, M. de; Nielsen, K.; Mattila-Holappa, P.; Mockałłod, Z.

    2016-01-01

    This is a review of published longitudinal empirical research on the impact of restructuring on employee well-being. We investigated whether restructuring accompanied by staff reductions impacts differently on worker well-being than restructuring without staff reductions, and the differences between

  1. Wrestling the Devil in the Details: An Early Look at Restructuring in California

    Science.gov (United States)

    Scott, Caitlin

    2006-01-01

    To learn more about district and school decision making for No Child Left Behind (NCLB) restructuring, the Center on Education Policy (CEP) turned to California, a state with a substantial number of schools in restructuring and several state and regional supports for making decisions about restructuring. In the summer and fall of 2005, CEP…

  2. 12 CFR 617.7415 - How does a qualified lender decide to restructure a loan?

    Science.gov (United States)

    2010-01-01

    ... restructure a loan? 617.7415 Section 617.7415 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM BORROWER RIGHTS Distressed Loan Restructuring; State Agricultural Loan Mediation Programs § 617.7415 How... the cost to the lender of restructuring the loan is equal to or less than the cost of foreclosure...

  3. Restructuring of the corporate sector of national economy: content and mechanism of realization

    Directory of Open Access Journals (Sweden)

    S. I. Gusakov

    2016-08-01

    It has been suggested to distinguish three directions of the restructuring: inner corporate restructuring; restructuring of relations; economics conditions restructuring. Inner corporate restructuring includes property forms changes, entity structure changes, capital structure changes, form of incorporation changes, management bodies changes, management changes, staff politics changes, reorganization, liquidation, privatization, corporatization. Restructuring of relations includes sales market changes, conversion of enterprises, diversification, contractor’s changes, property assignation, lease, concession, franchising, debt’s restructuring, changes of credit politics, technical supplement, using alternative power sources, standardization etc. Economics conditions restructuring includes regulation and legislation changes, tax regulation changes, currency market changes, changes in credit policies, changers in stock market. Accordingly, every restructuring direction includes many mechanisms of its possible fulfillment. The author has distinguished twenty six mechanisms all the restructuring and they are presented in the table form.The corporate management models’ use in correlation to corporate restructuring models has been described in the article. The author has come to the conclusion that pragmatic model of the corporate restructuring is more preferable in comparison with world recognized valuable model because of the peculiarities of the post-social economy in Ukraine.

  4. System development and asset management under restructuring

    International Nuclear Information System (INIS)

    2007-01-01

    In many developed countries the electricity infrastructure contains ageing assets. Because of the need of a continuing operation and higher load factors on the network, the opportunities for replacement or refurbishment are limited. These must be regarded as an opportunity to review the structure of the system and to consider the design and development required to meet the new requirements, taking advantage of new available technologies: HVDC, FACTS, cables, etc. The symposium examines assets management issues and assesses strategies for overhauling existing systems, taking into account the impact on market operation, on supply security and environment

  5. Economic-efficiency considerations in restructuring electric markets

    Energy Technology Data Exchange (ETDEWEB)

    Hill, L.J.

    1996-12-01

    In response to the Energy Policy Act of 1992 and the Federal Energy Regulatory Commission`s subsequent rulemaking on transmission access, many states are exploring options to restructure their electric industries. In their deliberations on restructuring, policymakers should consider (1) the reliability of the electric system; (2) income-distribution effects on ratepayers and utilities; (3) social consequences such as effects on energy conservation, renewable energy, and the environment; and (4) economic efficiency. We address economic-efficiency considerations in this study. Economic efficiency is important because it is one of the primary reasons that policymakers should consider restructuring in the first place: improving the electric-industry`s efficiency lowers costs and, hence, electric prices. In this study, we look at the sources of (in)efficiency in existing and proposed electric markets with the objective of guiding policymakers to design efficient electric markets. The advantages of a competitive market are well known: it leads to lower costs for the utility, lower prices for consumers, more product choices, better customer service, and often the need for less regulation by federal and state agencies. In the short run, firms who cannot produce at the market-clearing price are forced to leave the industry, ensuring that customers have the lowest price possible. In the long run, competition promotes innovation and lower costs. The physical and institutional characteristics of the U.S. electric industry, however, could be impediments to attaining efficiently run, competitive markets. Because of these characteristics, there are multiple sources of efficiencies and inefficiencies in existing electric markets, and there will be multiple sources in restructured ones. The objective of policymakers should not be to trade one set of inefficiencies in existing electric markets for another set in restructured markets.

  6. Economic-efficiency considerations in restructuring electric markets

    International Nuclear Information System (INIS)

    Hill, L.J.

    1996-12-01

    In response to the Energy Policy Act of 1992 and the Federal Energy Regulatory Commission's subsequent rulemaking on transmission access, many states are exploring options to restructure their electric industries. In their deliberations on restructuring, policymakers should consider (1) the reliability of the electric system; (2) income-distribution effects on ratepayers and utilities; (3) social consequences such as effects on energy conservation, renewable energy, and the environment; and (4) economic efficiency. We address economic-efficiency considerations in this study. Economic efficiency is important because it is one of the primary reasons that policymakers should consider restructuring in the first place: improving the electric-industry's efficiency lowers costs and, hence, electric prices. In this study, we look at the sources of (in)efficiency in existing and proposed electric markets with the objective of guiding policymakers to design efficient electric markets. The advantages of a competitive market are well known: it leads to lower costs for the utility, lower prices for consumers, more product choices, better customer service, and often the need for less regulation by federal and state agencies. In the short run, firms who cannot produce at the market-clearing price are forced to leave the industry, ensuring that customers have the lowest price possible. In the long run, competition promotes innovation and lower costs. The physical and institutional characteristics of the U.S. electric industry, however, could be impediments to attaining efficiently run, competitive markets. Because of these characteristics, there are multiple sources of efficiencies and inefficiencies in existing electric markets, and there will be multiple sources in restructured ones. The objective of policymakers should not be to trade one set of inefficiencies in existing electric markets for another set in restructured markets

  7. Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism.

    Science.gov (United States)

    Yu, Li; Sun, Yifan; Li, Jingjing; Wang, Yan; Zhu, Yuxing; Shi, Yong; Fan, Xiaojun; Zhou, Jianda; Bao, Ying; Xiao, Jie; Cao, Ke; Cao, Peiguo

    2017-08-15

    Radiotherapy has been used increasingly to treat primary hepatocellular carcinoma. Clinically, the main cause of radiotherapy failure is cellular radioresistance, conferred via glycolytic metabolism. Our previous study demonstrated that Girdin is upregulated in primary hepatocellular carcinoma and promotes the invasion and metastasis of tumor cells. However, whether Girdin underlies the radio-sensitivity of hepatocellular carcinoma remains unclear. A short hairpin RNA (shRNA) was used to silence CCDC88A (encoding Girdin), and real-time PCR was performed to determine CCDC88A mRNA expression. Then, cell proliferation, colony formation, flow cytometric, scratch, and transwell assays were to examine the influence of Girdin silencing on cellular radiosensitivity. Glycolysis assays were conducted to exam cell glycolysis process. Western blotting was performed to explore the signaling pathway downstream of Girdin. Finally, animal experiments were performed to demonstrate the effect of CCDC88A silencing on the radiosensitivity of hepatoma in vivo. shRNA-induced Girdin silencing suppressed glycolysis and enhanced the radio-sensitivity of hepatic cell lines, HepG2 and Huh-7. Furthermore, silencing of Girdin inhibited the PI3K/AKT/HIF-1α signaling pathway, which is a central regulator of glycolysis. Girdin can regulate glycolysis in hepatocellular carcinoma cells through the PI3K/AKT/HIF-1α signaling pathway, which decreases the sensitivity of tumor cells to radiotherapy.

  8. Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

    DEFF Research Database (Denmark)

    Jacobs, Robert A; Díaz, Víctor; Soldini, Lavinia

    2013-01-01

    The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirom......The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high......-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks......) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085...

  9. Autoimmunity against a glycolytic enzyme as a possible cause for persistent symptoms in Lyme disease.

    Science.gov (United States)

    Maccallini, Paolo; Bonin, Serena; Trevisan, Giusto

    2018-01-01

    Some patients with a history of Borrelia burgdorferi infection develop a chronic symptomatology characterized by cognitive deficits, fatigue, and pain, despite antibiotic treatment. The pathogenic mechanism that underlines this condition, referred to as post-treatment Lyme disease syndrome (PTLDS), is currently unknown. A debate exists about whether PTLDS is due to persistent infection or to post-infectious damages in the immune system and the nervous system. We present the case of a patient with evidence of exposure to Borrelia burgdorferi sl and a long history of debilitating fatigue, cognitive abnormalities and autonomic nervous system issues. The patient had a positive Western blot for anti-basal ganglia antibodies, and the autoantigen has been identified as γ enolase, the neuron-specific isoenzyme of the glycolytic enzyme enolase. Assuming Borrelia own surface exposed enolase as the source of this autoantibody, through a mechanism of molecular mimicry, and given the absence of sera reactivity to α enolase, a bioinformatical analysis was carried out to identify a possible cross-reactive conformational B cell epitope, shared by Borrelia enolase and γ enolase, but not by α enolase. Taken that evidence, we hypothesize that this autoantibody interferes with glycolysis in neuronal cells, as the physiological basis for chronic symptoms in at least some cases of PTLDS. Studies investigating on the anti-γ enolase and anti-Borrelia enolase antibodies in PTLDS are needed to confirm our hypotheses. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Blocking CD147 induces cell death in cancer cells through impairment of glycolytic energy metabolism

    International Nuclear Information System (INIS)

    Baba, Miyako; Inoue, Masahiro; Itoh, Kazuyuki; Nishizawa, Yasuko

    2008-01-01

    CD147 is a multifunctional transmembrane protein and promotes cancer progression. We found that the anti-human CD147 mouse monoclonal antibody MEM-M6/1 strongly induces necrosis-like cell death in LoVo, HT-29, WiDr, and SW620 colon cancer cells and A2058 melanoma cells, but not in WI-38 and TIG-113 normal fibroblasts. Silencing or overexpression of CD147 in LoVo cells enhanced or decreased the MEM-M6/1 induced cell death, respectively. CD147 is known to form complex with proton-linked monocarboxylate transporters (MCTs), which is critical for lactate transport and intracellular pH (pHi) homeostasis. In LoVo cells, CD147 and MCT-1 co-localized on the cell surface, and MEM-M6/1 inhibited the association of these molecules. MEM-M6/1 inhibited lactate uptake, lactate release, and reduced pHi. Further, the induction of acidification was parallel to the decrease of the glycolytic flux and intracellular ATP levels. These effects were not found in the normal fibroblasts. As cancer cells depend on glycolysis for their energy production, CD147 inhibition might induce cell death specific to cancer cells

  11. GLYCOLYTIC AND ENERGETIC RESOURCES AS THE DETERMINANTS OF MEAT QUALITY OF DUROC FATTENERS

    Directory of Open Access Journals (Sweden)

    Andrzej Zybert

    2015-03-01

    Full Text Available The aim of this study was to determine the diagnostic value of glycolityc and energetical quantities on selected meat quality characteristics of Duroc fatteners. A total of 40 Duroc porkers were investigated. Among two analysed sets of determinants (R1 with glycogen and R1 with lactate measured in 45 min after slaughter, the best diagnostic value for meat quality characteristics exhibit a set involving R1 and glycogen that composed determination coefficient (RC2 was 0.66 for pH measured in 45 min up to 144 h post mortem. Also, with currently used meat diagnostic methods the most useful one that explains the glycolytic and energetic quantities in the highest degree is method that exploits 5 determinants, i.e. pH1, pH24, EC2, EC24 and L*24. Higher composed determination coefficient and canonical correlation (CR for this method was obtained for set involving R1 and lactate – 0.41 and 0.64** respectively.

  12. Monitoring of glycolytic activity secondary to ischaemia in knee replacement surgery.

    Science.gov (United States)

    León-Muñoz, V J; Lisón-Almagro, A J; Hernández-García, C H; López-López, M

    2018-04-17

    To non-invasively assess tissue lesion secondary to ischaemia applied during knee replacement surgery. Secondary objectives: to assess whether this lesion correlates with the duration of ischaemia and whether instrumental and gender variables influence it. Prospective cohort study. Pre and postoperative serum lactate levels have been determined as an indicator of glycolytic activity secondary to ischaemia in 88 patients. Serum lactate determination was performed by reactive strips of enzymatic-amperometric detection on capillary blood. Preoperative serum lactate levels (mean and SD): 2.467±1.036 mmol/L. Postoperative serum lactate levels: 3.938±2.018 mmol/L. Ischaemia time 102.98±18.25minutes. Postoperative serum lactate levels were significantly higher than preoperative lactate levels. There are no statistical differences according to the time that the ischaemia was prolonged, gender or type of instrumentation used. In our study, postoperative serum lactate values were significantly higher than preoperative lactate values, with no correlation to the duration of ischaemia during knee replacement surgery. Copyright © 2018 SECOT. Publicado por Elsevier España, S.L.U. All rights reserved.

  13. Merkel Cell Polyomavirus Small T Antigen Promotes Pro-Glycolytic Metabolic Perturbations Required for Transformation.

    Directory of Open Access Journals (Sweden)

    Christian Berrios

    2016-11-01

    Full Text Available Merkel cell polyomavirus (MCPyV is an etiological agent of Merkel cell carcinoma (MCC, a highly aggressive skin cancer. The MCPyV small tumor antigen (ST is required for maintenance of MCC and can transform normal cells. To gain insight into cellular perturbations induced by MCPyV ST, we performed transcriptome analysis of normal human fibroblasts with inducible expression of ST. MCPyV ST dynamically alters the cellular transcriptome with increased levels of glycolytic genes, including the monocarboxylate lactate transporter SLC16A1 (MCT1. Extracellular flux analysis revealed increased lactate export reflecting elevated aerobic glycolysis in ST expressing cells. Inhibition of MCT1 activity suppressed the growth of MCC cell lines and impaired MCPyV-dependent transformation of IMR90 cells. Both NF-κB and MYC have been shown to regulate MCT1 expression. While MYC was required for MCT1 induction, MCPyV-induced MCT1 levels decreased following knockdown of the NF-κB subunit RelA, supporting a synergistic activity between MCPyV and MYC in regulating MCT1 levels. Several MCC lines had high levels of MYCL and MYCN but not MYC. Increased levels of MYCL was more effective than MYC or MYCN in increasing extracellular acidification in MCC cells. Our results demonstrate the effects of MCPyV ST on the cellular transcriptome and reveal that transformation is dependent, at least in part, on elevated aerobic glycolysis.

  14. Vulnerability of network of networks

    Science.gov (United States)

    Havlin, S.; Kenett, D. Y.; Bashan, A.; Gao, J.; Stanley, H. E.

    2014-10-01

    Our dependence on networks - be they infrastructure, economic, social or others - leaves us prone to crises caused by the vulnerabilities of these networks. There is a great need to develop new methods to protect infrastructure networks and prevent cascade of failures (especially in cases of coupled networks). Terrorist attacks on transportation networks have traumatized modern societies. With a single blast, it has become possible to paralyze airline traffic, electric power supply, ground transportation or Internet communication. How, and at which cost can one restructure the network such that it will become more robust against malicious attacks? The gradual increase in attacks on the networks society depends on - Internet, mobile phone, transportation, air travel, banking, etc. - emphasize the need to develop new strategies to protect and defend these crucial networks of communication and infrastructure networks. One example is the threat of liquid explosives a few years ago, which completely shut down air travel for days, and has created extreme changes in regulations. Such threats and dangers warrant the need for new tools and strategies to defend critical infrastructure. In this paper we review recent advances in the theoretical understanding of the vulnerabilities of interdependent networks with and without spatial embedding, attack strategies and their affect on such networks of networks as well as recently developed strategies to optimize and repair failures caused by such attacks.

  15. Modulation of cellular radiation responses by 2-deoxy-D-glucose and other glycolytic inhibitors: Implications for cancer therapy

    OpenAIRE

    Kalia Vijay; Prabhakara S; Narayanan Vidya

    2009-01-01

    Background: 2-Deoxy-D-glucose (2-DG), a glycolytic inhibitor, was observed earlier to increase DNA, chromosomal, and cellular damage in tumor cells, by inhibiting energy-dependent repair processes. Lonidamine (LND) selectively inhibits glycolysis in cancer cells. It damages the condensed mitochondria in these cells, impairing thereby the activity of hexokinase (predominantly attached to the outer mitochondrial membranes). It inhibits repair of radiation-induced potentially lethal cellular da...

  16. Negative Effect of Ellagic Acid on Cytosolic pH Regulation and Glycolytic Flux in Human Endometrial Cancer Cells

    OpenAIRE

    Khalid N. M. Abdelazeem; Yogesh Singh; Florian Lang; Madhuri S. Salker

    2017-01-01

    Background/Aims: Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions, which is in part accomplished by Na+/H+ exchangers, such as NHE1. The carrier is sensitive to oxidative stress. Growth of tumor cells could be suppressed by the polyphenol Ellagic acid, which is found in various fruits and vegetables. An effect of ...

  17. Overexpression of Genes Encoding Glycolytic Enzymes in Corynebacterium glutamicum Enhances Glucose Metabolism and Alanine Production under Oxygen Deprivation Conditions

    Science.gov (United States)

    Yamamoto, Shogo; Gunji, Wataru; Suzuki, Hiroaki; Toda, Hiroshi; Suda, Masako; Jojima, Toru; Inui, Masayuki

    2012-01-01

    We previously reported that Corynebacterium glutamicum strain ΔldhAΔppc+alaD+gapA, overexpressing glyceraldehyde-3-phosphate dehydrogenase-encoding gapA, shows significantly improved glucose consumption and alanine formation under oxygen deprivation conditions (T. Jojima, M. Fujii, E. Mori, M. Inui, and H. Yukawa, Appl. Microbiol. Biotechnol. 87:159–165, 2010). In this study, we employ stepwise overexpression and chromosomal integration of a total of four genes encoding glycolytic enzymes (herein referred to as glycolytic genes) to demonstrate further successive improvements in C. glutamicum glucose metabolism under oxygen deprivation. In addition to gapA, overexpressing pyruvate kinase-encoding pyk and phosphofructokinase-encoding pfk enabled strain GLY2/pCRD500 to realize respective 13% and 20% improved rates of glucose consumption and alanine formation compared to GLY1/pCRD500. Subsequent overexpression of glucose-6-phosphate isomerase-encoding gpi in strain GLY3/pCRD500 further improved its glucose metabolism. Notably, both alanine productivity and yield increased after each overexpression step. After 48 h of incubation, GLY3/pCRD500 produced 2,430 mM alanine at a yield of 91.8%. This was 6.4-fold higher productivity than that of the wild-type strain. Intracellular metabolite analysis showed that gapA overexpression led to a decreased concentration of metabolites upstream of glyceraldehyde-3-phosphate dehydrogenase, suggesting that the overexpression resolved a bottleneck in glycolysis. Changing ratios of the extracellular metabolites by overexpression of glycolytic genes resulted in reduction of the intracellular NADH/NAD+ ratio, which also plays an important role on the improvement of glucose consumption. Enhanced alanine dehydrogenase activity using a high-copy-number plasmid further accelerated the overall alanine productivity. Increase in glycolytic enzyme activities is a promising approach to make drastic progress in growth-arrested bioprocesses. PMID

  18. Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance[C][W

    Science.gov (United States)

    Barkla, Bronwyn J.; Vera-Estrella, Rosario; Hernández-Coronado, Marcela; Pantoja, Omar

    2009-01-01

    To examine the role of the tonoplast in plant salt tolerance and identify proteins involved in the regulation of transporters for vacuolar Na+ sequestration, we exploited a targeted quantitative proteomics approach. Two-dimensional differential in-gel electrophoresis analysis of free flow zonal electrophoresis separated tonoplast fractions from control, and salt-treated Mesembryanthemum crystallinum plants revealed the membrane association of glycolytic enzymes aldolase and enolase, along with subunits of the vacuolar H+-ATPase V-ATPase. Protein blot analysis confirmed coordinated salt regulation of these proteins, and chaotrope treatment indicated a strong tonoplast association. Reciprocal coimmunoprecipitation studies revealed that the glycolytic enzymes interacted with the V-ATPase subunit B VHA-B, and aldolase was shown to stimulate V-ATPase activity in vitro by increasing the affinity for ATP. To investigate a physiological role for this association, the Arabidopsis thaliana cytoplasmic enolase mutant, los2, was characterized. These plants were salt sensitive, and there was a specific reduction in enolase abundance in the tonoplast from salt-treated plants. Moreover, tonoplast isolated from mutant plants showed an impaired ability for aldolase stimulation of V-ATPase hydrolytic activity. The association of glycolytic proteins with the tonoplast may not only channel ATP to the V-ATPase, but also directly upregulate H+-pump activity. PMID:20028841

  19. The capital structure of banks and practice of bank restructuring : eight case studies on current bank restructurings in Europe ; final report

    OpenAIRE

    Dübel, Hans-Joachim

    2013-01-01

    This study presents an empirical analysis of capital and liability management in eight cases of bank restructurings and resolutions from eight different European countries. It can be read as a companion piece to an earlier study by the author covering the specific bank restructuring programs of Greece, Spain and Cyprus during 2012/13. The study portrays for each case the timelines between the initial credit event and the (last) restructuring. It proceeds to discuss the capital and liabilit...

  20. Lithium prevents early cytosolic calcium increase and secondary injurious calcium overload in glycolytically inhibited endothelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Bosche, Bert, E-mail: bert.bosche@uk-essen.de [Department of Neurology, University of Duisburg-Essen (Germany); Max Planck Institute for Neurological Research with Klaus-Joachim-Zülch Laboratories of the Max Planck Society and the Medical Faculty of the University of Cologne (Germany); Schäfer, Matthias, E-mail: matthias.schaefer@sanofi.com [Institute of Physiology, Justus-Liebig-University Giessen (Germany); Graf, Rudolf, E-mail: rudolf.graf@nf.mpg.de [Max Planck Institute for Neurological Research with Klaus-Joachim-Zülch Laboratories of the Max Planck Society and the Medical Faculty of the University of Cologne (Germany); Härtel, Frauke V., E-mail: frauke.haertel@tu-dresden.de [Institute of Physiology, Medical Faculty Carl Gustav Carus, Technical University Dresden (Germany); Schäfer, Ute, E-mail: ute.schaefer@medunigraz.at [Research Unit for Experimental Neurotraumatology, Medical University of Graz (Austria); Noll, Thomas, E-mail: thomas.noll@tu-dresden.de [Institute of Physiology, Medical Faculty Carl Gustav Carus, Technical University Dresden (Germany)

    2013-05-03

    Highlights: •We investigate free calcium as a central signalling element in endothelial cells. •Inhibition of glycolysis with 2-deoxy-D-glucose reduces cellular ATP. •This manoeuvre leads to a biphasic increase and overload of free calcium. •Pre-treatment with lithium for 24 h abolishes both phases of the calcium increase. •This provides a new strategy to protect endothelial calcium homeostasis and barrier function. -- Abstract: Cytosolic free calcium concentration ([Ca{sup 2+}]{sub i}) is a central signalling element for the maintenance of endothelial barrier function. Under physiological conditions, it is controlled within narrow limits. Metabolic inhibition during ischemia/reperfusion, however, induces [Ca{sup 2+}]{sub i} overload, which results in barrier failure. In a model of cultured porcine aortic endothelial monolayers (EC), we addressed the question of whether [Ca{sup 2+}]{sub i} overload can be prevented by lithium treatment. [Ca{sup 2+}]{sub i} and ATP were analysed using Fura-2 and HPLC, respectively. The combined inhibition of glycolytic and mitochondrial ATP synthesis by 2-desoxy-D-glucose (5 mM; 2-DG) plus sodium cyanide (5 mM; NaCN) caused a significant decrease in cellular ATP content (14 ± 1 nmol/mg protein vs. 18 ± 1 nmol/mg protein in the control, n = 6 culture dishes, P < 0.05), an increase in [Ca{sup 2+}]{sub i} (278 ± 24 nM vs. 71 ± 2 nM in the control, n = 60 cells, P < 0.05), and the formation of gaps between adjacent EC. These observations indicate that there is impaired barrier function at an early state of metabolic inhibition. Glycolytic inhibition alone by 10 mM 2-DG led to a similar decrease in ATP content (14 ± 2 nmol/mg vs. 18 ± 1 nmol/mg in the control, P < 0.05) with a delay of 5 min. The [Ca{sup 2+}]{sub i} response of EC was biphasic with a peak after 1 min (183 ± 6 nM vs. 71 ± 1 nM, n = 60 cells, P < 0.05) followed by a sustained increase in [Ca{sup 2+}]{sub i}. A 24-h pre-treatment with 10 mM of lithium

  1. The Glycolytic Versatility of Bacteroides uniformis CECT 7771 and Its Genome Response to Oligo and Polysaccharides

    Directory of Open Access Journals (Sweden)

    Alfonso Benítez-Páez

    2017-08-01

    Full Text Available Bacteroides spp. are dominant components of the phylum Bacteroidetes in the gut microbiota and prosper in glycan enriched environments. However, knowledge of the machinery of specific species isolated from humans (like Bacteroides uniformis contributing to the utilization of dietary and endogenous sources of glycans and their byproducts is limited. We have used the cutting-edge nanopore-based technology to sequence the genome of B. uniformis CECT 7771, a human symbiont with a proven pre-clinical efficacy on metabolic and immune dysfunctions in obesity animal models. We have also used massive sequencing approaches to distinguish the genome expression patterns in response to carbon sources of different complexity during growth. At genome-wide level, our analyses globally demonstrate that B. uniformis strains exhibit an expanded glycolytic capability when compared with other Bacteroides species. Moreover, by studying the growth and whole-genome expression of B. uniformis CECT 7771 in response to different carbon sources, we detected a differential growth fitness and expression patterns across the genome depending on the carbon source of the culture media. The dietary fibers used exerted different effects on B. uniformis CECT 7771 activating different molecular pathways and, therefore, allowing the production of different metabolite types with potential impact on gut health. The genome and transcriptome analysis of B. uniformis CECT 7771, in response to different carbon sources, shows its high versatility to utilize both dietary and endogenous glycans along with the production of potentially beneficial end products for both the bacterium and the host, pointing to a mechanistic basis of a mutualistic relationship.

  2. Subcellular localization of glycolytic enzymes and characterization of intermediary metabolism of Trypanosoma rangeli.

    Science.gov (United States)

    Rondón-Mercado, Rocío; Acosta, Héctor; Cáceres, Ana J; Quiñones, Wilfredo; Concepción, Juan Luis

    2017-09-01

    Trypanosoma rangeli is a hemoflagellate protist that infects wild and domestic mammals as well as humans in Central and South America. Although this parasite is not pathogenic for human, it is being studied because it shares with Trypanosoma cruzi, the etiological agent of Chagas' disease, biological characteristics, geographic distribution, vectors and vertebrate hosts. Several metabolic studies have been performed with T. cruzi epimastigotes, however little is known about the metabolism of T. rangeli. In this work we present the subcellular distribution of the T. rangeli enzymes responsible for the conversion of glucose to pyruvate, as determined by epifluorescense immunomicroscopy and subcellular fractionation involving either selective membrane permeabilization with digitonin or differential and isopycnic centrifugation. We found that in T. rangeli epimastigotes the first six enzymes of the glycolytic pathway, involved in the conversion of glucose to 1,3-bisphosphoglycerate are located within glycosomes, while the last four steps occur in the cytosol. In contrast with T. cruzi, where three isoenzymes (one cytosolic and two glycosomal) of phosphoglycerate kinase are expressed simultaneously, only one enzyme with this activity is detected in T. rangeli epimastigotes, in the cytosol. Consistent with this latter result, we found enzymes involved in auxiliary pathways to glycolysis needed to maintain adenine nucleotide and redox balances within glycosomes such as phosphoenolpyruvate carboxykinase, malate dehydrogenase, fumarate reductase, pyruvate phosphate dikinase and glycerol-3-phosphate dehydrogenase. Glucokinase, galactokinase and the first enzyme of the pentose-phosphate pathway, glucose-6-phosphate dehydrogenase, were also located inside glycosomes. Furthermore, we demonstrate that T. rangeli epimastigotes growing in LIT medium only consume glucose and do not excrete ammonium; moreover, they are unable to survive in partially-depleted glucose medium. The

  3. Restructuring the Russian electricity sector: Re-creating California?

    International Nuclear Information System (INIS)

    Pittman, Russell

    2007-01-01

    The Russian Federation has begun restructuring its electricity sector, following the standard restructuring model of complete vertical separation of generation from transmission, with the aim of creating competition in regional generation markets. This paper examines the structure of the six principal regional generation markets that are in their early stages of development and argues that they are likely to be characterized by high levels of market power on the part of individual privatized generation companies, especially during the peak winter demand season. These levels-considerably higher than those that caused competitive problems in California-seem to create a serious risk of price spikes in deregulated wholesale electricity markets, and thus of significant price increases to consumers of electricity

  4. An experimental investigation of glare and restructured fiber metal laminates

    Science.gov (United States)

    Benedict, Adelina Vanessa

    Fiber Metal Laminates (FMLs) are a group of materials fabricated by bonding glass/epoxy layers within metal layers. This class of materials can provide good mechanical properties, as well as weight savings. An FML known as Glass Laminate Aluminum Reinforced Epoxy (GLARE) was studied. An experimental investigation comprising of microscopy and tensile testing was carried out using different grades of GLARE. Microscopy revealed the construction details of GLARE, while tensile testing provided means of measuring and analyzing its stress-strain responses. Next, different metal surface pretreatment methods were explored. These included sandblasting, Phosphoric Acid Anodizing (PAA), and AC-130 Sol-Gel treatment. Woven S-2 glass, an epoxy adhesive, and aluminum alloy sheet metal were used to fabricate restructured FMLs using time and cost effective procedures. Additional microscopy and tensile testing allowed for comparisons with GLARE and aircraft grade aluminum alloys. The restructured FMLs showed similar behaviors to GLARE with potential significant improvements in fabrication efficiency.

  5. Restructuring the US telecommunications industry - Impact on innovation

    Science.gov (United States)

    Agnew, C. E.; Romeo, A. A.

    1981-01-01

    The Communications Act of 1934, which regulates the United States telecommunications industry, is becoming less able to deal with the changes brought by modern technology. Therefore, proposals are being made to restructure the industry. Breaking up AT&T into smaller firms would have only minor effects, since its ratio of R and D to sales has been near the median for large telephone companies. Restructuring AT&T into subsidiaries dealing at arms' length might cause a reordering of priorities toward more directly marketable and useful products, but too extensive a break-up could endanger Bell Labs' commitment to basic research. Regulation leads a firm to ignore true social value of possible products, expand into low profit markets, and favor capital-intensive over labor-intensive innovation. If regulatory lag occurs, a firm will produce cost-reducing innovation in order to increase its profit.

  6. The impact of electricity restructuring on the natural gas industry

    International Nuclear Information System (INIS)

    Given, G.

    1999-03-01

    The main objective of the study is to quantify the impact of electrical restructuring on the natural gas industry, in particular the expected rise in natural gas consumption for electricity generation in Canada from 2000 to 2020. To this end, the study estimates how large the demand potential is and where it is likely to materialize, with the timing of these events also considered. Chapter two discusses the study methodology, which is that of a quantitative forecast. Before an analytical model was chosen, a careful review of potential candidates, a literature review, and an analysis of important issues were undertaken. Chapter three provides requisite background information of electricity restructuring issues. U.K. experiences are highlighted, and common issues and differences among states and provinces are discussed also. Chapter four provides some necessary historical background and puts into perspective the importance of gas consumption for electric generation. Also treated are relevant Canadian and U.S. generation and capacity, and forecasts of restructuring impacts from other organizations. Whether a consensus forecast for gas demand exists is examined, as well as a number of critical factors examined in various studies. Chapter five describes the development of individual study scenarios and key assumptions contained in each scenario. Also described are some of the selected model's capabilities. Retail and generation company strategy parameters are highlighted to provide a better understanding of those capabilities. The competition parameters chosen for generators and retail companies in the model are described. Chapter six reports all key forecast variables in a discussion which compares relevant scenario and sensitivity forecast results. Detailed forecast results for two scenarios are provided in appendices. Chapter seven discusses new investment and business opportunities and challenges in light of the forecast results. These are reviewed for a number of

  7. Investigation of an automatic trim algorithm for restructurable aircraft control

    Science.gov (United States)

    Weiss, J.; Eterno, J.; Grunberg, D.; Looze, D.; Ostroff, A.

    1986-01-01

    This paper develops and solves an automatic trim problem for restructurable aircraft control. The trim solution is applied as a feed-forward control to reject measurable disturbances following control element failures. Disturbance rejection and command following performances are recovered through the automatic feedback control redesign procedure described by Looze et al. (1985). For this project the existence of a failure detection mechanism is assumed, and methods to cope with potential detection and identification inaccuracies are addressed.

  8. Three essays on U.S. electricity restructuring

    Science.gov (United States)

    Sergici, Sanem I.

    2008-04-01

    The traditional structure of the electricity sector in the U.S. has been that of large vertically integrated companies with sole responsibility for distributing power to end users within a franchise area. The restructuring of this sector that has occurred in the past 10-20 years has profoundly altered this picture. This dissertation examines three aspects of that restructuring process. First chapter of my dissertation investigates the impacts of divestitures of generation, an important part of the process of restructuring, on the efficiency of distribution systems. We find that while all divestitures as a group do not significantly affect distribution efficiency, those mandated by state public utility commissions have resulted in large and statistically significant adverse effects on distribution efficiency. Second chapter of my dissertation explores whether independent system operator (ISO) formation in New York has led to operating efficiencies at the unit and the system level. ISOs oversee the centralized management of the grid and the energy market and are expected to promote more efficient power generation. We test these efficiencies focusing on the generation units in New York ISO region from 1998 to 2004 and find that the NYISO formation has introduced limited efficiencies at the unit and the system level. Restructuring in the electricity industry has spawned a new wave of mergers, both raising questions and providing opportunities to examine these mergers. Third chapter of my dissertation investigates the drivers of electric utility mergers consummated between 1992 and 2004. My results provide support for disturbance theory of mergers, size hypothesis, and inefficient management hypothesis as drivers of electric utility mergers. I also find that the adjacency of the service territories is the most noteworthy determinant of the pairings between IOUs.

  9. Transmission investment and expansion planning in a restructured electricity market

    International Nuclear Information System (INIS)

    Wu, F.F; Wen, F.S.; Zheng, F.L.

    2006-01-01

    Transmission planning in a restructured electricity market becomes increasingly complicated. To bridge the gap between economic and engineering considerations, this survey paper suggests a framework to clarify the interactions among various economic and engineering issues by reviewing recent theoretical and practical progress in transmission investment and transmission planning methodology. Thus, the paper makes economic literature more accessible to the engineering community and engineering literature more accessible to the economic community interested in the subject. (author)

  10. Corporate Finance and Restructuring: Evidence from Central and Eastern Europe

    OpenAIRE

    Köke, F. Jens; Salem, Tanja

    2000-01-01

    After the end of communism enterprises in Central and Eastern Europe (CEE) were marked by low levels of labor productivity, mainly because of too high employment levels. According to economic theory, the corporate capital structure can be an important element in the restructuring process. But both, empirical evidence on corporate finance in CEE countries and its relation to employment is still sparse. This study describes the patterns of the corporate capital structure for ten CEE countries o...

  11. The organizational restructuring performative act under shareholder value management ideology

    OpenAIRE

    Patrícia Saltorato; Glauco Benatti

    2017-01-01

    Purpose – This paper’s objective is to present the dynamics involving an organizational restructuring process conducted in a Brazilian subsidiary of a centenary American industrial corporation which claimed to be seeking, by means of this process, to increase the value of the company’s shares, but, which results reveal the distance between the promises and outcomes of this process, unveiling the symbolic-performative nature of such a process. Methodology – The chosen research method was t...

  12. Power system restructuring and deregulation: trading, performance and information technology

    International Nuclear Information System (INIS)

    Loi Lei Lai

    2001-09-01

    Representatives from several countries have contributed to a book addressing the deregulation and restructuring of the electric power industry. Articles covered include guidance on asset management, transmission balancing and meter management systems, tools for studying competitive power markets, environmental impacts, costs and benefits, and the new strategies and technology available for power generation, transmission and distribution. The book should be of interest to power systems engineers, system operators, managers, planners and policy makers in the electric power business

  13. The Iron and Steel Industry in Asia: Development and Restructuring

    OpenAIRE

    Sato, Hajime

    2009-01-01

    The paper examines the development and restructuring of the iron and steel industryin Asian countries. Studying countries that have integrated steelworks with largeblast furnaces (South Korea, Taiwan, China and India) and countries without(Thailand, Indonesia and Malaysia), the paper shows the difference in thedevelopment processes across the countries and across time, and points to thediversity of the development experience of these countries. The paper argues thatsignificant differences in ...

  14. Electric restructuring: Observations about what is in the public interest

    Energy Technology Data Exchange (ETDEWEB)

    Hoecker, J.

    1996-12-31

    Opinions regarding restructuring of the U.S. electric utility industry are presented in the paper. A brief assessment is made of Federal Energy Regulatory Commission orders requiring open access transmission services and open access same-time information systems. Three subtopics are pursued in some detail: competition between renewables and conservation, the role of government, and the impact of government on the market for renewables. It is concluded that renewable programs can be incorporated into competitive markets through regulatory agencies.

  15. Investigation of nuclear multifragmentation using molecular dynamics and restructured aggregation

    International Nuclear Information System (INIS)

    Paula, L. de; Nemeth, J.; Ben-Hao, Sa.; Leray, S.; Ngo, C.; Souza, S.R.; Yu-Ming, Zheng; Paula, L. de; Nemeth, J.; Ben-Hao, Sa.; Yu-Ming, Zheng; Ngo, H.

    1991-01-01

    We study the stability of excited 197 Au nuclei with respect to multifragmentation. For that we use a dynamical simulation based on molecular dynamics and restructured aggregation. A particular attention is paid to check the stability of the ground state nuclei generated by the simulation. Four kinds of excitations are considered: heat, compression, rotation and a geometrical instability created when a projectile drills a hole in a 197 Au nucleus

  16. PERFORMANCE ET RESTRUCTURATION: LE CAS SAÏDAL ...

    African Journals Online (AJOL)

    performance de SAÏDAL suite à sa restructuration débutée en 1997. Le concept de ... millions d'actions. Du point de vue du management, SAÏDAL adopte le tableau de .... Méthodes et Pratiques de la Performance : Le Guide du Pilotage. ... performance des entreprises, Kaplan et Norton proposèrent le tableau de bord ...

  17. Cognitive restructuring of gambling-related thoughts: A systematic review.

    Science.gov (United States)

    Chrétien, Maxime; Giroux, Isabelle; Goulet, Annie; Jacques, Christian; Bouchard, Stéphane

    2017-12-01

    Gamblers' thoughts have a fundamental influence on their gambling problem. Cognitive restructuring is the intervention of choice to correct those thoughts. However, certain difficulties are noted in the application of cognitive restructuring techniques and the comprehension of their guidelines. Furthermore, the increase of skill game players (e.g. poker) entering treatment creates a challenge for therapists, as these gamblers present with different thoughts than those of the gamblers usually encountered in treatment (e.g. chance-only games like electronic gambling machines). This systematic review aims to describe how cognitive restructuring is carried out with gamblers based on the evidence available in empirical studies that include cognitive interventions for gambling. Of the 2607 studies collected, 39 were retained. The results highlight exposure as the most frequently used technique to facilitate identification of gambling-related thoughts (imaginal=28.2%; in vivo=10.3%). More than half of the studies (69.2%) clearly reported therapeutic techniques aimed to correct gamblers' thoughts, of which 37% involved visual support to challenge those thoughts (e.g. ABC log). Of the 39 studies retained, 48.7% included skill game players (i.e., poker, blackjack, sports betting) in their sample. However, none of these studies mentioned whether cognitive restructuring had been adapted for these gamblers. Several terms referring to gamblers' thoughts were used interchangeably (e.g. erroneous, dysfunctional or inadequate thoughts), although each of these terms could refer to specific content. Clinical implications of the results are discussed with regard to the needs of therapists. This review also suggests recommendations for future research. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Soft restructuring process in metallurgical enterprises in Poland

    Directory of Open Access Journals (Sweden)

    B. Gajdzik

    2015-10-01

    Full Text Available This article presents the range and outcomes of soft restructuring in metallurgical enterprises in Poland. The term ‘soft restructuring’ applies to changes in metallurgical enterprises’ employment policy during the period of political transformation in Poland. Steelworks performance under the market economy conditions demanded introducing changes in staff resources. Changes referred both to the staff structure as well as employees’ skills and gradual engaging of the staff in building the steelworks’ competitive advantage.

  19. Greening public power : protecting the public interest in electricity restructuring

    International Nuclear Information System (INIS)

    Stewart, K.

    2002-01-01

    On April 30, 2002, the Toronto Environmental Alliance (TEA) called for a moratorium on the sale of Ontario's electricity system and called for new policies to protect the environment. The TEA is critical of the government's plan to privatise and deregulate the province's electricity system, given the experience with restructuring in the United States and Europe. The TEA argues that the plan to deregulate will promote the production of more electricity from polluting coal-fired power plants and nuclear power generating stations, increasing health risks. It was also argued that restructuring creates barriers to introducing green power from renewable resources such as wind and solar energy. The government's plan to restructure will create markets that are easily manipulated by large private power companies to increase profits and eliminate small green power providers. It was also suggested that once electric power generation is privatised, it will be subjected to the rules of the North American Free Trade Agreement (NAFTA), which prioritize profits over environmental protection. This report presents some environmental policies of other jurisdictions that have proven to save consumers money, provide security and jobs, while doing so in an environmentally sustainable manner. 29 refs

  20. Automatic control design procedures for restructurable aircraft control

    Science.gov (United States)

    Looze, D. P.; Krolewski, S.; Weiss, J.; Barrett, N.; Eterno, J.

    1985-01-01

    A simple, reliable automatic redesign procedure for restructurable control is discussed. This procedure is based on Linear Quadratic (LQ) design methodologies. It employs a robust control system design for the unfailed aircraft to minimize the effects of failed surfaces and to extend the time available for restructuring the Flight Control System. The procedure uses the LQ design parameters for the unfailed system as a basis for choosing the design parameters of the failed system. This philosophy alloys the engineering trade-offs that were present in the nominal design to the inherited by the restructurable design. In particular, it alloys bandwidth limitations and performance trade-offs to be incorporated in the redesigned system. The procedure also has several other desirable features. It effectively redistributes authority among the available control effectors to maximize the system performance subject to actuator limitations and constraints. It provides a graceful performance degradation as the amount of control authority lessens. When given the parameters of the unfailed aircraft, the automatic redesign procedure reproduces the nominal control system design.

  1. Major Thought Restructuring: The Roles of Different Prefrontal Cortical Regions.

    Science.gov (United States)

    Seyed-Allaei, Shima; Avanaki, Zahra Nasiri; Bahrami, Bahador; Shallice, Tim

    2017-07-01

    An important question for understanding the neural basis of problem solving is whether the regions of human prefrontal cortices play qualitatively different roles in the major cognitive restructuring required to solve difficult problems. However, investigating this question using neuroimaging faces a major dilemma: either the problems do not require major cognitive restructuring, or if they do, the restructuring typically happens once, rendering repeated measurements of the critical mental process impossible. To circumvent these problems, young adult participants were challenged with a one-dimensional Subtraction (or Nim) problem [Bouton, C. L. Nim, a game with a complete mathematical theory. The Annals of Mathematics, 3, 35-39, 1901] that can be tackled using two possible strategies. One, often used initially, is effortful, slow, and error-prone, whereas the abstract solution, once achieved, is easier, quicker, and more accurate. Behaviorally, success was strongly correlated with sex. Using voxel-based morphometry analysis controlling for sex, we found that participants who found the more abstract strategy (i.e., Solvers) had more gray matter volume in the anterior medial, ventrolateral prefrontal, and parietal cortices compared with those who never switched from the initial effortful strategy (i.e., Explorers). Removing the sex covariate showed higher gray matter volume in Solvers (vs. Explorers) in the right ventrolateral prefrontal and left parietal cortex.

  2. A restructuring of CF package for MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S. H.; Kim, K. R.; Kim, D. H.; Cho, S. W.

    2004-01-01

    CF package, which evaluates user-specified 'control functions' and applies them to define or control various aspects of computation, has been restructured for the MIDAS computer code. MIDAS is being developed as an integrated severe accident analysis code with a user-friendly graphical user interface and modernized data structure. To do this, data transferring methods of current MELCOR code are modified and adopted into the CF package. The data structure of the current MELCOR code using FORTRAN77 causes a difficult grasping of meaning of the variables as well as waste of memory, difficulty is more over because its data is location information of other package's data due to characteristics of CF package. New features of FORTRAN90 make it possible to allocate the storage dynamically and to use the user-defined data type, which lead to an efficient memory treatment and an easy understanding of the code. Restructuring of the CF package addressed in this paper includes module development, subroutine modification, and treats MELGEN, which generates data file, as well as MELCOR, which is processing a calculation. The verification has been done by comparing the results of the modified code with those from the existing code. As the trends are similar to each other, it hints that the same approach could be extended to the entire code package. It is expected that code restructuring will accelerate the code domestication thanks to direct understanding of each variable and easy implementation of modified or newly developed models

  3. On program restructuring, scheduling, and communication for parallel processor systems

    Energy Technology Data Exchange (ETDEWEB)

    Polychronopoulos, Constantine D. [Univ. of Illinois, Urbana, IL (United States)

    1986-08-01

    This dissertation discusses several software and hardware aspects of program execution on large-scale, high-performance parallel processor systems. The issues covered are program restructuring, partitioning, scheduling and interprocessor communication, synchronization, and hardware design issues of specialized units. All this work was performed focusing on a single goal: to maximize program speedup, or equivalently, to minimize parallel execution time. Parafrase, a Fortran restructuring compiler was used to transform programs in a parallel form and conduct experiments. Two new program restructuring techniques are presented, loop coalescing and subscript blocking. Compile-time and run-time scheduling schemes are covered extensively. Depending on the program construct, these algorithms generate optimal or near-optimal schedules. For the case of arbitrarily nested hybrid loops, two optimal scheduling algorithms for dynamic and static scheduling are presented. Simulation results are given for a new dynamic scheduling algorithm. The performance of this algorithm is compared to that of self-scheduling. Techniques for program partitioning and minimization of interprocessor communication for idealized program models and for real Fortran programs are also discussed. The close relationship between scheduling, interprocessor communication, and synchronization becomes apparent at several points in this work. Finally, the impact of various types of overhead on program speedup and experimental results are presented.

  4. A restructuring of RN1 package for MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S. H.; Kim, D. H.; Kim, K. R.

    2003-01-01

    RN1 package, which is one of two fission product-related packages in MELCOR, has been restructured for the MIDAS computer code. MIDAS is being developed as an integrated severe accident analysis code with a user-friendly graphical user interface and modernized data structure. To do this, data transferring methods of current MELCOR code are modified and adopted into the RN1 package. The data structure of the current MELCOR code using FORTRAN77 causes a difficult grasping of meaning of the variables as well as waste of memory. New features of FORTRAN90 make it possible to allocate the storage dynamically and to use the user-defined data type, which lead to an efficient memory treatment and an easy understanding of the code. Restructuring of the RN1 package addressed in this paper includes module development, subroutine modification, and treats MELGEN, which generates data file, as well as MELCOR, which is processing a calculation. The verification has been done by comparing the results of the modified code with those from the existing code. As the trends are similar to each other, it hints that the same approach could be extended to the entire code package. It is expected that code restructuring will accelerate the code domestication thanks to direct understanding of each variable and easy implementation of modified or newly developed models

  5. A restructuring of COR package for MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S.H.; Kim, K.R.; Kim, D.H.

    2004-01-01

    The COR package, which calculates the thermal response of the core and the lower plenum internal structures and models the relocation of the core and lower plenum structural materials, has been restructured for the MIDAS computer code. MIDAS is being developed as an integrated severe accident analysis code with a user-friendly graphical user interface and a modernized data structure. To do this, the data transferring methods of the current MELCOR code are modified and adopted into the COR package. The data structure of the current MELCOR code using FORTRAN77 has a difficulty in grasping the meaning of the variables as well as a waste of memory. New features of FORTRAN90 make it possible to allocate the storage dynamically and to use the user-defined data type, which leads to an efficient memory treatment and an easy understanding of the code. Restructuring of the COR package addressed in this paper includes a module development, subroutine modification. The verification has been done by comparing the results of the modified code with those of the existing code. As the trends are similar to each other, it implies that the same approach could be extended to the entire code package. It is expected that the code restructuring will accelerated the code's domestication thanks to a direct understanding of each variable and an easy implementation of the modified or newly developed models. (author)

  6. A restructuring of TF package for MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S. H.; Song, Y. M.; Kim, D. H.

    2002-01-01

    TF package which defines some interpolation and extrapolation condition through user defined table has been restructured in MIDAS computer code. To do this, data transferring methods of current MELCOR code are modified and adopted into TF package. The data structure of the current MELCOR code using FORTRAN77 causes a difficult grasping of the meaning of the variables as well as waste of memory. New features of FORTRAN90 make it possible to allocate the storage dynamically and to use the user-defined data type, which lead to an efficient memory treatment and an easy understanding of the code. Restructuring of TF package addressed in this paper does module development and subroutine modification, and treats MELGEN which is making restart file as well as MELCOR which is processing calculation. The validation has been done by comparing the results of the modified code with those from the existing code, and it is confirmed that the results are the same. It hints that the similar approach could be extended to the entire code package. It is expected that code restructuring will accelerate the code domestication thanks to direct understanding of each variable and easy implementation of modified or newly developed models

  7. A restructuring of RN2 package for MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S. H.; Kim, D. H.

    2003-01-01

    RN2 package, which is one of two fission product-related package in MELCOR, has been restructured for the MIDAS computer code. MIDAS is being developed as an integrated severe accident analysis code with a user-friendly graphical user interface and data structure. To do this, data transferring methods of current MELCOR code are modified and adopted into the RN2 package. The data structure of the current MELCOR code using FORTRAN77 causes a difficult grasping of meaning of the variables as well as waste of memory. New features of FORTRAN90 make it possible to allocate the storage dynamically and to use the user-defined data type, which lead to an efficient memory treatment and an easy understanding of the code. Restructuring of the RN2 package addressed in this paper includes module development, subroutine modification, and treats MELGEN, which generates data file, as well as MELCOR, which is processing a calculation. The validation has been done by comparing the results of the modified code with those from the existing code. As the trends are the similar to each other, it hints that the same approach could be extended to the entire code package. It is expected that code restructuring will accelerate the code domestication thanks to direct understanding of each variable and easy implementation of modified or newly developed models

  8. Restructuring and energy efficiency improvement of the Bulgarian energy economy

    International Nuclear Information System (INIS)

    Moumdjian, G.

    1993-01-01

    The structure of the national energy economy of Bulgaria implies characteristic features that specify low efficiency as regards power production, ecology and economics. Even the qualitative assessments show that these indices stand far away from the standards established in developed countries like Denmark, Finland, Sweden, etc. The best starting position for harmful energy efficiency improvement as well as emission reduction must be based on the restructuring of energy economy. The strategy of restructuring and development of energy economy covers the whole integrated national energy flow system 'resources - end user'. The preliminary study shows that energy efficiency can be increased by 25-30% within a period of 6-10 years using the least-cost investment strategy (including the research and development activities expenses). The study covers the existing structure of energy sector. Scenarios are being elaborated for its development and restructuring in respect to: heat production and transfer; electricity generation and transmission; energy consumption and conservation in residential buildings, public buildings and commercial sector; energy consumption in transport sector and agriculture. The approach for identification of the real potential opportunities in relation to the above stated areas is based on mathematical statistics and stochastic differential equations, multicriterial assessments, approach of self organisation systems and demand-side management. (author)

  9. American Indian tribes and electric industry restructuring: Issues and opportunities

    Energy Technology Data Exchange (ETDEWEB)

    Howarth, D. [Morse, Richard, and Weisenmiller, and Associates Inc., Oakland, CA (United States); Busch, J. [Lawrence Berkeley National Lab., CA (United States); Starrs, T. [Kelso, Starrs, and Associates LLC, Vashon, WA (United States)

    1997-07-01

    The US electric utility industry is undergoing a period of fundamental change that has significant implications for Native American tribes. Although many details remain to be determined, the future electric power industry will be very different from that of the present. It is anticipated that the new competitive electric industry will be more efficient, which some believe will benefit all participants by lowering electricity costs. Recent developments in the industry, however, indicate that the restructuring process will likely benefit some parties at the expense of others. Given the historical experience and current situation of Native American tribes in the US, there is good reason to pay attention to electric industry changes to ensure that the situation of tribes is improved and not worsened as a result of electric restructuring. This paper provides a review of electricity restructuring in the US and identifies ways in which tribes may be affected and how tribes may seek to protect and serve their interests. Chapter 2 describes the current status of energy production and service on reservations. Chapter 3 provides an overview of the evolution of the electric industry to its present form and introduces the regulatory and structural changes presently taking place. Chapter 4 provides a more detailed discussion of changes in the US electric industry with a specific focus on the implications of these changes for tribes. Chapter 5 presents a summary of the conclusions reached in this paper.

  10. Power market restructuring in Asia : Russia, China, India, and Japan

    International Nuclear Information System (INIS)

    Hammons, T.J.; Zhong, J.; Mukhopadhyay, S.; Kurihara, I.

    2008-01-01

    Many countries are now in the process of deregulating their power industries in order to promote growth and competitiveness. This paper discussed power market restructuring activities in Russia, China, India and Japan. Economic convergence points in Russian and Asian power markets were reviewed. The state of Russia and China's power industry after the implementation of recent power restructuring initiatives was discussed. Technical characteristics of the industries were evaluated, and market development plans were outlined. Regional electricity markets in Asia were discussed, as well as issues related to domestic and foreign investment. Institutional reforms were reviewed, and individual outlines of revisions for the power industries of the 4 countries were presented. The study demonstrated that structuring processes vary from country to country. Differences in restructuring patterns were attributed to economic differences; country-specific features established within the electric power industry; and attitudes towards deregulation. It was concluded that the reforms adopted by the countries will lead to the expansion of national electric power systems. 23 refs., 4 tabs., 6 figs

  11. Hyperoxia decreases glycolytic capacity, glycolytic reserve and oxidative phosphorylation in MLE-12 cells and inhibits complex I and II function, but not complex IV in isolated mouse lung mitochondria.

    Directory of Open Access Journals (Sweden)

    Kumuda C Das

    Full Text Available High levels of oxygen (hyperoxia are frequently used in critical care units and in conditions of respiratory insufficiencies in adults, as well as in infants. However, hyperoxia has been implicated in a number of pulmonary disorders including bronchopulmonary dysplasia (BPD and adult respiratory distress syndrome (ARDS. Hyperoxia increases the generation of reactive oxygen species (ROS in the mitochondria that could impair the function of the mitochondrial electron transport chain. We analyzed lung mitochondrial function in hyperoxia using the XF24 analyzer (extracellular flux and optimized the assay for lung epithelial cells and mitochondria isolated from lungs of mice. Our data show that hyperoxia decreases basal oxygen consumption rate (OCR, spare respiratory capacity, maximal respiration and ATP turnover in MLE-12 cells. There was significant decrease in glycolytic capacity and glycolytic reserve in MLE-12 cells exposed to hyperoxia. Using mitochondria isolated from lungs of mice exposed to hyperoxia or normoxia we have shown that hyperoxia decreased the basal, state 3 and state3 μ (respiration in an uncoupled state respirations. Further, using substrate or inhibitor of a specific complex we show that the OCR via complex I and II, but not complex IV was decreased, demonstrating that complexes I and II are specific targets of hyperoxia. Further, the activities of complex I (NADH dehydrogenase, NADH-DH and complex II (succinate dehydrogenase, SDH were decreased in hyperoxia, but the activity of complex IV (cytochrome oxidase, COX remains unchanged. Taken together, our study show that hyperoxia impairs glycolytic and mitochondrial energy metabolism in in tact cells, as well as in lungs of mice by selectively inactivating components of electron transport system.

  12. Networking

    OpenAIRE

    Rauno Lindholm, Daniel; Boisen Devantier, Lykke; Nyborg, Karoline Lykke; Høgsbro, Andreas; Fries, de; Skovlund, Louise

    2016-01-01

    The purpose of this project was to examine what influencing factor that has had an impact on the presumed increasement of the use of networking among academics on the labour market and how it is expressed. On the basis of the influence from globalization on the labour market it can be concluded that the globalization has transformed the labour market into a market based on the organization of networks. In this new organization there is a greater emphasis on employees having social qualificati...

  13. Genetic disruption of lactate/H+ symporters (MCTs) and their subunit CD147/BASIGIN sensitizes glycolytic tumor cells to phenformin.

    Science.gov (United States)

    Marchiq, Ibtissam; Le Floch, Renaud; Roux, Danièle; Simon, Marie-Pierre; Pouyssegur, Jacques

    2015-01-01

    Rapidly growing glycolytic tumors require energy and intracellular pH (pHi) homeostasis through the activity of two major monocarboxylate transporters, MCT1 and the hypoxia-inducible MCT4, in intimate association with the glycoprotein CD147/BASIGIN (BSG). To further explore and validate the blockade of lactic acid export as an anticancer strategy, we disrupted, via zinc finger nucleases, MCT4 and BASIGIN genes in colon adenocarcinoma (LS174T) and glioblastoma (U87) human cell lines. First, we showed that homozygous loss of MCT4 dramatically sensitized cells to the MCT1 inhibitor AZD3965. Second, we demonstrated that knockout of BSG leads to a decrease in lactate transport activity of MCT1 and MCT4 by 10- and 6-fold, respectively. Consequently, cells accumulated an intracellular pool of lactic and pyruvic acids, magnified by the MCT1 inhibitor decreasing further pHi and glycolysis. As a result, we found that these glycolytic/MCT-deficient cells resumed growth by redirecting their metabolism toward OXPHOS. Third, we showed that in contrast with parental cells, BSG-null cells became highly sensitive to phenformin, an inhibitor of mitochondrial complex I. Phenformin addition to these MCT-disrupted cells in normoxic and hypoxic conditions induced a rapid drop in cellular ATP-inducing cell death by "metabolic catastrophe." Finally, xenograft analysis confirmed the deleterious tumor growth effect of MCT1/MCT4 ablation, an action enhanced by phenformin treatment. Collectively, these findings highlight that inhibition of the MCT/BSG complexes alone or in combination with phenformin provides an acute anticancer strategy to target highly glycolytic tumors. This genetic approach validates the anticancer potential of the MCT1 and MCT4 inhibitors in current development. ©2014 American Association for Cancer Research.

  14. Effect of hypoxia on the activity and binding of glycolytic and associated enzymes in sea scorpion tissues

    Directory of Open Access Journals (Sweden)

    Lushchak V.I.

    1998-01-01

    Full Text Available The effect of hypoxia on the levels of glycogen, glucose and lactate as well as the activities and binding of glycolytic and associated enzymes to subcellular structures was studied in brain, liver and white muscle of the teleost fish, Scorpaena porcus. Hypoxia exposure decreased glucose levels in liver from 2.53 to 1.70 µmol/g wet weight and in muscle led to its increase from 3.64 to 25.1 µmol/g wet weight. Maximal activities of several enzymes in brain were increased by hypoxia: hexokinase by 23%, phosphoglucoisomerase by 47% and phosphofructokinase (PFK by 56%. However, activities of other enzymes in brain as well as enzymes in liver and white muscle were largely unchanged or decreased during experimental hypoxia. Glycolytic enzymes in all three tissues were partitioned between soluble and particulate-bound forms. In several cases, the percentage of bound enzymes was reduced during hypoxia; bound aldolase in brain was reduced from 36.4 to 30.3% whereas glucose-6-phosphate dehydrogenase fell from 55.7 to 28.7% bound. In muscle PFK was reduced from 57.4 to 41.7% bound. Oppositely, the proportion of bound aldolase and triosephosphate isomerase increased in hypoxic muscle. Phosphoglucomutase did not appear to occur in a bound form in liver and bound phosphoglucomutase disappeared in muscle during hypoxia exposure. Anoxia exposure also led to the disappearance of bound fructose-1,6-bisphosphatase in liver, whereas a bound fraction of this enzyme appeared in white muscle of anoxic animals. The possible function of reversible binding of glycolytic enzymes to subcellular structures as a regulatory mechanism of carbohydrate metabolism is discussed.

  15. WE-FG-BRA-08: Potential Role of the Glycolytic Oscillator in Acute Hypoxia in Tumors

    International Nuclear Information System (INIS)

    Che Fru, L; Adamson, E; Campos, D; Song, C; Kimple, R; Fain, S; Kissick, M; Jacques, S; Kogel, A van der; Nickel, K

    2016-01-01

    Purpose: Oscillatory dynamics in acute hypoxia have been observed, but poorly understood. They have mostly been attributed to vascular perturbations, but no link has yet been made to metabolic causes. We set out to determine the fundamental frequencies and test for coherence in tumor oxygen dynamics and spatial properties. Methods: Severe combined immunodeficient (SCID) mice were inoculated onto bilateral flanks with human derived head and neck carcinoma (UW-SCC22) cell line xenografts. Oxygen dynamics were monitored in the tumor every minute for an hour using three modalities: blood oxygen level dependent - magnetic resonance imaging (BOLD-MRI), hemoglobin oxygen saturation photoacoustic, and locally manufactured optical probes for spectral fitting. A statistical test was used to separate fluctuating from non-fluctuating voxels and pixels in BOLD-MRI and photoacoustic data respectively. The power spectrum density (PSD) and the autocorrelation functions were calculated for the time series of each voxel, pixel and region, of the BOLD-MRI, photoacoustic or fiber optic data respectively. Results: Using all three techniques, intermittent oxygen dynamics with both coherent and incoherent signatures was observed in the tumors. Upon averaging the PSDs of fluctuating voxels and pixels, it was found that these oscillations occurred with periods of minutes to tens of minutes from all three approaches. Observations from the BOLD-MRI and photoacoustic data showed that clusters of voxels oscillated in a synchronized manner. Conclusion: We were able to use three different modalities to show that fluctuation in tumor oxygen is both coherent and incoherent, with periods of minutes to tens of minutes. These periods are very similar to those from the well-established metabolic, non-linear biomechanical phenomenon called the glycolytic oscillator. This may provide an additional explanation to the cause of cyclic hypoxia. Such dynamics could have profound implications in

  16. WE-FG-BRA-08: Potential Role of the Glycolytic Oscillator in Acute Hypoxia in Tumors

    Energy Technology Data Exchange (ETDEWEB)

    Che Fru, L [University of Wisconsin, Madison, Madison, WI (United States); Adamson, E; Campos, D; Song, C; Kimple, R [University of Wisconsin Madison, Madison, WI (United States); Fain, S; Kissick, M [University of Wisconsin, Madison, WI (United States); Jacques, S [Oregon Health and Science University, Portland OR USA, Portland, OR (United States); Kogel, A van der [University of Wisconsin - Madison, Madison, WI (United States); Nickel, K [University of Wisconsin Madison, Madison, Wisconsin (United States)

    2016-06-15

    Purpose: Oscillatory dynamics in acute hypoxia have been observed, but poorly understood. They have mostly been attributed to vascular perturbations, but no link has yet been made to metabolic causes. We set out to determine the fundamental frequencies and test for coherence in tumor oxygen dynamics and spatial properties. Methods: Severe combined immunodeficient (SCID) mice were inoculated onto bilateral flanks with human derived head and neck carcinoma (UW-SCC22) cell line xenografts. Oxygen dynamics were monitored in the tumor every minute for an hour using three modalities: blood oxygen level dependent - magnetic resonance imaging (BOLD-MRI), hemoglobin oxygen saturation photoacoustic, and locally manufactured optical probes for spectral fitting. A statistical test was used to separate fluctuating from non-fluctuating voxels and pixels in BOLD-MRI and photoacoustic data respectively. The power spectrum density (PSD) and the autocorrelation functions were calculated for the time series of each voxel, pixel and region, of the BOLD-MRI, photoacoustic or fiber optic data respectively. Results: Using all three techniques, intermittent oxygen dynamics with both coherent and incoherent signatures was observed in the tumors. Upon averaging the PSDs of fluctuating voxels and pixels, it was found that these oscillations occurred with periods of minutes to tens of minutes from all three approaches. Observations from the BOLD-MRI and photoacoustic data showed that clusters of voxels oscillated in a synchronized manner. Conclusion: We were able to use three different modalities to show that fluctuation in tumor oxygen is both coherent and incoherent, with periods of minutes to tens of minutes. These periods are very similar to those from the well-established metabolic, non-linear biomechanical phenomenon called the glycolytic oscillator. This may provide an additional explanation to the cause of cyclic hypoxia. Such dynamics could have profound implications in

  17. Do all types of restructuring threaten employees' well-being? An exploratory study.

    Science.gov (United States)

    Widerszal-Bazyl, Maria; Mockałło, Zofia

    2015-01-01

    Most research on the negative impact of restructuring on employees' health considers restructuring involving personnel reduction. The aim of this study was to explore the assumption that the type of restructuring, business expansion versus restructuring not involving expansion (only reductions and/or change of ownership), influences its psychological responses: appraisal of the change, psychosocial working conditions and well-being after the change. The study was carried out among 857 employees that experienced restructuring in 2009 and/or 2010 and 538 employees from companies not restructured at that time. The main variables, i.e., assessment of change in terms of personal benefits and losses, psychosocial job characteristics and well-being were measured using a questionnaire developed in "The psychological health and well-being in restructuring: key effects and mechanisms" project (PSYRES). It was found that the employees who experienced business expansion in comparison to those who experienced exclusively change of ownership had a higher appraisal of change, while those who experienced restructuring not involving business expansion did not differ from those who experienced change of ownership. As far as psychosocial working conditions are concerned, those employees who experienced exclusively business expansion did not differ from those in the not restructured companies (except for quantitative demands that were higher), while most psychosocial working conditions of the employees who experienced restructuring not involving expansion were poorer than in the not restructured companies. Also, well-being measures of the employees who experienced exclusively business expansion did not differ from those in the not restructured companies (except for innovative behavior that was even higher), while well-being measure of those who experienced restructuring not involving expansion was poorer than of those in the not restructured companies. Restructuring involving

  18. A Restructuring of the HS Package for the MIDAS Computer Code

    International Nuclear Information System (INIS)

    Park, S. H.; Kim, K. R.; Kim, D. H.; Cho, S. W.

    2005-01-01

    As one of the processes for a localized severe accident analysis code, KAERI is developing a severe accident code MIDAS, based on MELCOR. The existing data saving method uses pointer variables for a fix-sized storage management, and it deteriorates the readability, maintainability and portability of the code. But new features in FORTRAN90 such as a dynamic allocation have been used for the restructuring. The restructuring of the data saving and transferring method of the existing code makes it easy to understand the code. Before an entire restructuring of the code, a restructuring template for a simple package was developed and tested. The target for the restructuring was the HS package which is responsible for calculation the heat conduction within an intact, solid structure and energy transfer across its boundary surfaces into control volumes. The verification was done through comparing the results before and after the restructuring

  19. A restructuring of the FL package for the MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S. H.; Kim, K. R.; Kim, D. H.; Cho, S. W.

    2005-01-01

    The developmental need for a localized severe accident analysis code is on the rise, and KAERI is developing a severe accident code MIDAS, based on MELCOR. The existing data saving method uses pointer variables for a fix-sized storage management, and it deteriorates the readability, maintainability and portability of the code. But new features in FORTRAN90 such as a dynamic allocation have been used for the restructuring. The restructuring of the data saving and transferring method of the existing code makes it easy to understand the code. Before an entire restructuring of the code, a restructuring template for a simple package was developed and tested. The target for the restructuring was the FL package which is responsible for modeling the thermal-hydraulic behavior of a liquid water, water vapor, and gases in MELCOR with the CVH package. The verification was done through comparing the results before and after the restructuring

  20. The role of distributed generation (DG) in a restructured utility environment

    International Nuclear Information System (INIS)

    Feibus, H.

    1999-01-01

    A major consequence of the restructuring of the electric utility industry is disintegration, by which the traditional integrated utility is spinning off its generation business and becoming a power distribution company, or distco. This company will be the remaining entity of the traditional electric utility that continues to be regulated. The world in which the distco functions is becoming a very different place. The distco will be called upon to deliver not only power, but a range of ancillary services, defined by the Federal Energy Regulatory Commission, including spinning reserves, voltage regulation, reactive power, energy imbalance and network stability, some of which may be obtained from the independent system operator, and some of which may be provided by the distco. In this environment the distco must maintain system reliability and provide service to the customer at the least cost. Meanwhile, restructuring is spawning a new generation of unregulated energy service companies that threaten to win the most attractive customers from the distco. Fortunately there is a new emerging generation of technologies, distributed resources, that provide options to the distco to help retain prime customers, by improving reliability and lowering costs. Specifically, distributed generation and storage systems if dispersed into the distribution system can provide these benefits, if generators with the right characteristics are selected, and the integration into the distribution system is done skillfully. The Electric Power Research Institute has estimated that new distributed generation may account for 30% of new generation. This presentation will include the characteristics of several distributed resources and identify potential benefits that can be obtained through the proper integration of distributed generation and storage systems

  1. Management, restructuring and industrial relations : organizational change within the United Kingdom broadcasting industry, 1979-2002

    OpenAIRE

    Quinn, Brian J.

    2007-01-01

    In the past decade researchers began to remedy a literature deficit in the study of broadcasting institutions – addressing in particular the issues of labour process, restructuring and industrial relations. This thesis which considers management change, industrial relations and restructuring within the United Kingdom (‘UK’) broadcasting industry employs a case study format with a view to highlighting the variations of industry (macro) and firm (micro) based restructuring. Organizational chang...

  2. THE ORGANIZATIONAL-ECONOMIC MECHANISM OF RESTRUCTURING OF THE MARKET OF MINERAL OIL IN REGION

    Directory of Open Access Journals (Sweden)

    V.I. Efimenkov

    2007-12-01

    Full Text Available Restructuring, in opinion of the experts, one of the basic ways of steady development of managing subjects in modern conditions. In clause strategy of organizational-economic restructuring of the regional market of mineral oil is considered. Concrete actions of complex restructuring of the market of mineral oil in region and the mechanism of management are resulted by reorganization of the given market.

  3. The association between patient safety culture and burnout and sense of coherence: A cross-sectional study in restructured and not restructured intensive care units.

    Science.gov (United States)

    Vifladt, Anne; Simonsen, Bjoerg O; Lydersen, Stian; Farup, Per G

    2016-10-01

    To study the associations between registered nurses' (RNs) perception of the patient safety culture (safety culture) and burnout and sense of coherence, and to compare the burnout and sense of coherence in restructured and not restructured intensive care units (ICUs). Cross-sectional study. RNs employed at seven ICUs in six hospitals at a Norwegian Hospital Trust. One to four years before the study, three hospitals merged their general and medical ICUs into one general mixed ICU. The safety culture, burnout and sense of coherence were measured with the questionnaires Hospital Survey on Patient Safety Culture, Bergen Burnout Indicator and Sense of Coherence. Participant characteristics and working in restructured and not restructured ICUs were registered. In total, 143/289(49.5%) RNs participated. A positive safety culture was statistically significantly associated with a low score for burnout and a strong sense of coherence. No statistically significant differences were found in burnout and sense of coherence between RNs in the restructured and not restructured ICUs. In this study, a positive safety culture was associated with absence of burnout and high ability to cope with stressful situations. Burnout and sense of coherence were independent of the restructuring process. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  4. Do generation firms in restructured electricity markets have incentives to support social-welfare-improving transmission investments?

    International Nuclear Information System (INIS)

    Sauma, Enzo E.; Oren, Shmuel S.

    2009-01-01

    This paper examines the incentives that generation firms have in restructured electricity markets for supporting long-term transmission investments. In particular, we study whether generation firms, which arguably play a dominant role in the restructured electricity markets, have the incentives to fund or support incremental social-welfare-improving transmission investments. We examine this question in a two-node network and explore how such incentives are affected by the ownership of financial transmission rights (FTRs) by generation firms. In the analyzed two-node network, we show both (1) that the net exporter generation firm has the correct incentives to increase the transmission capacity incrementally up to a certain level and (2) that, although a policy that allocates FTRs to the net exporter generation firm can be desirable from a social point of view, such a policy would dilute the net-importer-generation-firm's incentives to support transmission expansion. Moreover, if all FTRs were allocated or auctioned off to the net exporter generation firm, then it is possible to increase both consumer surplus and social welfare while keeping the net exporter generation firm revenue neutral. (author)

  5. Strategic plan for the restructured US fusion energy sciences program

    International Nuclear Information System (INIS)

    1996-08-01

    This plan reflects a transition to a restructured fusion program, with a change in focus from an energy technology development program to a fusion energy sciences program. Since the energy crisis of the early 1970's, the U.S. fusion program has presented itself as a goal- oriented fusion energy development program, with milestones that required rapidly increasing budgets. The Energy Policy Act of 1992 also called for a goal-oriented development program consistent with the Department's planning. Actual funding levels, however, have forced a premature narrowing of the program to the tokamak approach. By 1995, with no clear, immediate need driving the schedule for developing fusion energy and with enormous pressure to reduce discretionary spending, Congress cut fusion program funding for FY 1996 by one-third and called for a major restructuring of the program. Based on the recommendations of the Fusion Energy Advisory Committee (FEAC), the Department has decided to pursue a program that concentrates on world-class plasma, science, and on maintaining an involvement in fusion energy science through international collaboration. At the same time, the Japanese and Europeans, with energy situations different from ours, are continuing with their goal- oriented fusion programs. Collaboration with them provides a highly leveraged means of continued involvement in fusion energy science and technology, especially through participation in the engineering and design activities of the International Thermonuclear Experimental Reactor program, ITER. This restructured fusion energy sciences program, with its focus on fundamental fusion science and technology, may well provide insights that lead to more attractive fusion power plants, and will make use of the scientific infrastructure that will allow the United States to launch a fusion energy development program at some future date

  6. SOVEREIGN DEBT RESTRUCTURING AND “VULTURE FUNDS”

    Directory of Open Access Journals (Sweden)

    Emilia Cornelia STOICA

    2016-06-01

    Full Text Available Defining sovereign debt - debt issued or guaranteed by a public entity: central and / or regional public authorities, central banks, public institutions or enterprises - must include the risks that its management may generate, mainly the risk of default. If an medium period of time - 3-5 years – the macroeconomic growth of a state, and as the result the increase of the public revenues constantly lies below the growth of sovereign debt, these will cause an insolvability risk to cover it, and that state should proceed to restructure its debt. Financial stability of public authorities and sovereign debt occurred since the beginning of the creation of democratic states, and instruments for debt restructuring have been continuously adapted to economic and social conjuncture. Initially, states faced a necessity of funding were borrowed from foreign governments and / or large consortia bank, and when their debts had to be restructured it has been created the international institutional framework to negotiate between debtor countries and public creditors - Paris Club - and to coordinate negotiations between public authorities and major debtor consortia - London Club. In the last decade 'vulture funds' occurred, which are hedge funds acquiring from the secondary financial market debt the securities, including public debt, to a much lower share nominal value. Subsequently, vulture funds claim states issuing debt repayment at values close or equal to the face value - in this way can make a profit of more than 100% of the financial investment they made it on the secondary market. If these countries do not comply, generally being unable to honor their public debt, vultures funds act the countries in international courts, which usually prevails because vultures funds’ action is legal under current conditions.

  7. European ecological networks and greenways

    NARCIS (Netherlands)

    Jongman, R.H.G.; Külvik, M.; Kristiansen, I.

    2004-01-01

    In the context of European integration, networks are becoming increasingly important in both social and ecological sense. Since the beginning of the 1990s, societal and scientific exchanges are being restructured as the conceptual approaches towards new nature conservation strategies have been

  8. Restructuring the industry sector - the impact on energy demand

    International Nuclear Information System (INIS)

    Constantinescu, M.

    1994-01-01

    The structure of the industrial sector is a factor of major importance in analyzing the evolution of energy intensity or in setting-up realistic development scenarios. A positive influence on the energy intensity value is expected for Romania from the process of restructuring the industry sector towards low energy consumption products. In order to reach this target though, suitable end comprehensive strategies have to become operational without delay, promoting energy efficiency and modern technologies at a nation-wide scale. The benefits of such strategies extend from improvement of the security of supply through environmental protection and reduction of unemployment. (Author)

  9. Energy resources and restructuring of electroenergetics of the Slovak Republic

    International Nuclear Information System (INIS)

    Fecko, S.; Janicek, F.; Revakova, D.; Simunek, P.

    1996-01-01

    The paper discusses the new energy policy of development of national power production in the context of the transition to free market economy. In the frame of this new policy there are presented the main objectives: privatization and restructuring of energetics in view of the concept of sustainable development of energy environment; utilization of new energy sources and implementing of more efficient energy distribution systems; ecologization of the energy production process according to international requirements and standards; rationalization of the energy consumption in order to meet all consumption needs, to lower the energetic demands of the national economy and reach positive ecological impacts. (author) 5 figs., 6 refs

  10. Optimized multi area AGC simulation in restructured power systems

    International Nuclear Information System (INIS)

    Bhatt, Praghnesh; Roy, Ranjit; Ghoshal, S.P.

    2010-01-01

    In this paper, the traditional automatic generation control loop with modifications is incorporated for simulating automatic generation control (AGC) in restructured power system. Federal energy regulatory commission (FERC) encourages an open market system for price based operation. FERC has issued a notice for proposed rulemaking of various ancillary services. One of these ancillary services is load following with frequency control which comes broadly under Automatic Generation Control in deregulated regime. The concept of DISCO participation matrix is used to simulate the bilateral contracts in the three areas and four area diagrams. Hybrid particle swarm optimization is used to obtain optimal gain parameters for optimal transient performance. (author)

  11. Effect of aspirin and prostaglandins on the carbohydrate metabolism in albino rats.: glucose oxidation through different pathways and glycolytic enzymes

    International Nuclear Information System (INIS)

    Balasubramanian, A.; Ramakrishnan, S.

    1980-01-01

    The effect of chronic and acute doses of aspirin and prostaglandins F2α and E2 individually on the oxidation of glucose through Embden Meyerhof-TCA cycle and pentose phosphate pathways and some key glycolytic enzymes of liver were studied in male albino rats. Studies were extended to find the combined effect of PGF2α and E2 with an acute dose of aspirin. There was increased utilisation of both 1- 14 C glucose and 6- 14 C glucose on aspirin treatment. However, the metabolism through the EM-TCA pathway was more pronounced as shown by a reduced ratio of 14 CO 2 from 1- 14 C and 6- 14 C glucose. Two hepatic key glycolytic enzymes viz. hexokinase and pyruvate kinase were increased due to aspirin treatment. Withdrawal of aspirin corrected the above impaired carbohydrate metabolism in liver. Prostaglandin F2α also caused a reduction in the utilisation of 1- 14 C glucose, while PGE2 recorded an increase in the utilisation of both 1- 14 C and 6- 14 C glucose when compared to controls, indicating that different members of prostaglandins could affect metabolisms and differently. Administration of the PGs and aspirin together showed an increase in the utilisation of 6- 14 C glucose. (auth.)

  12. Effects of Interval Training-Based Glycolytic Capacity on Physical Fitness in Recreational Long-Distance Runners

    Directory of Open Access Journals (Sweden)

    Zatoń Marek

    2015-06-01

    Full Text Available Purpose. The aim of this study was to investigate the influence of 8-week-long interval training (targeting glycolytic capacity on selected markers of physical fitness in amateur long-distance runners. Methods. The study involved 17 amateur long-distance runners randomly divided into an experimental (n = 8 and control (n = 9 group. The control group performed three or four continuous training sessions per week whereas the experimental group performed two interval running training sessions and one continuous running training session. A graded treadmill exercise test and the 12-min Cooper test were performed pre- and post-training. Results. O2max and the rate of recovery increased in the experimental group. Relative oxygen uptake, minute ventilation, and heart rate speed decreased in low- (6 km/h and medium-intensity (12 km/h running. Conclusions. Both training modalities showed similar results. However, the significant differences in training volume (4-8 min interval training vs. 40-150 min continuous training indicates that the modalities targeting glycolytic capacity may be more efficient for amateur runners prepare for long-distance events.

  13. New insights into the complex regulation of the glycolytic pathway in Lactococcus lactis. I. Construction and diagnosis of a comprehensive dynamic model.

    Science.gov (United States)

    Dolatshahi, Sepideh; Fonseca, Luis L; Voit, Eberhard O

    2016-01-01

    This article and the companion paper use computational systems modeling to decipher the complex coordination of regulatory signals controlling the glycolytic pathway in the dairy bacterium Lactococcus lactis. In this first article, the development of a comprehensive kinetic dynamic model is described. The model is based on in vivo NMR data that consist of concentration trends in key glycolytic metabolites and cofactors. The model structure and parameter values are identified with a customized optimization strategy that uses as its core the method of dynamic flux estimation. For the first time, a dynamic model with a single parameter set fits all available glycolytic time course data under anaerobic operation. The model captures observations that had not been addressed so far and suggests the existence of regulatory effects that had been observed in other species, but not in L. lactis. The companion paper uses this model to analyze details of the dynamic control of glycolysis under aerobic and anaerobic conditions.

  14. Metabolomic Analysis of Oxidative and Glycolytic Skeletal Muscles by Matrix-Assisted Laser Desorption/IonizationMass Spectrometric Imaging (MALDI MSI)

    Science.gov (United States)

    Tsai, Yu-Hsuan; Garrett, Timothy J.; Carter, Christy S.; Yost, Richard A.

    2015-06-01

    Skeletal muscles are composed of heterogeneous muscle fibers that have different physiological, morphological, biochemical, and histological characteristics. In this work, skeletal muscles extensor digitorum longus, soleus, and whole gastrocnemius were analyzed by matrix-assisted laser desorption/ionization mass spectrometry to characterize small molecule metabolites of oxidative and glycolytic muscle fiber types as well as to visualize biomarker localization. Multivariate data analysis such as principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to extract significant features. Different metabolic fingerprints were observed from oxidative and glycolytic fibers. Higher abundances of biomolecules such as antioxidant anserine as well as acylcarnitines were observed in the glycolytic fibers, whereas taurine and some nucleotides were found to be localized in the oxidative fibers.

  15. Examining Escherichia coli glycolytic pathways, catabolite repression, and metabolite channeling using Δpfk mutants

    DEFF Research Database (Denmark)

    Hollinshead, Whitney D.; Rodriguez, Sarah; Martin, Hector Garcia

    2016-01-01

    Background: Glycolysis breakdowns glucose into essential building blocks and ATP/NAD(P)H for the cell, occupying a central role in its growth and bio-production. Among glycolytic pathways, the Entner Doudoroff pathway (EDP) is a more thermodynamically favorable pathway with fewer enzymatic steps...... directed through the EDP (~20 % of glycolysis flux). Disrupting the EMPP by phosphofructokinase I (pfkA) knockout increased flux through OPPP (~60 % of glycolysis flux) and the native EDP (~14 % of glycolysis flux), while overexpressing edd and eda in this ΔpfkA mutant directed ~70 % of glycolytic flux...... in glycolysis intermediates, possibly suggesting metabolite channeling (metabolites in glycolysis are pass from enzyme to enzyme without fully equilibrating within the cytosol medium). Conclusions: We engineered E. coli to redistribute its native glycolytic flux. The replacement of EMPP by EDP did not improve E...

  16. The Peculiar Glycolytic Pathway in Hyperthermophylic Archaea : Understanding Its Whims by Experimentation In Silico

    NARCIS (Netherlands)

    Zhang, Y.; Kouril, T.; Snoep, J.L.; Siebers, B.; Barberis, M.; Westerhoff, H.V.

    2017-01-01

    Mathematical models are key to systems biology where they typically describe the topology and dynamics of biological networks, listing biochemical entities and their relationships with one another. Some (hyper)thermophilic Archaea contain an enzyme, called non-phosphorylating

  17. Judicial System Restructuring and Modernization in Abu Dhabi

    Directory of Open Access Journals (Sweden)

    Lawrence Groo

    2008-01-01

    Full Text Available The purpose of this article is to provide a practical overview of the recently initiated modernization of Abu Dhabi’s judicial system. Beginning in 2007, Abu Dhabi’s Government launched a comprehensive effort to transform the Emirate’s judicial system. While the implementation of these reforms is ongoing, with the adoption of the law in May 2007 establishing the new judicial architecture the initial phase of the modernization program is already complete. The restructuring process encompasses court management and administration reform, a new judicial training regime, a redesigned organizational structure for the Emirate’s Judicial Department and courts, and the establishment of a system-wide strategic planning and budgeting process. Many of these initiatives are supported by applying advanced IT-based applications. Given the early achievements and ambitious broader aims of the restructuring process, Abu Dhabi’s example is relevant not only to the other Emirates within the Federal UAE system, but also within the context of the wider Middle East region.

  18. Restructuring, ownership and efficiency in the electricity industry

    Science.gov (United States)

    Shanefelter, Jennifer Kaiser

    The first chapter considers improvements in productive efficiency that can result from a movement from a regulated framework to one that allows for market-based incentives for industry participants. Specifically, I look at the case of restructuring in the electricity generation industry. Using data from the electricity industry, this analysis considers the total effect of restructuring on one input to the production process, labor, as reflected in employment levels, payroll per employee and aggregate establishment payroll. Using concurrent payroll and employment data from non-utility ("merchant") and utility generators in both restructured and nonrestructured states, I estimate the effect of market liberalization, comprising both new entry and state-level legislation, on employment and payroll in this industry. I find that merchant owners of divested generation assets employ significantly fewer people, but that the payroll per employee is not significantly different from what workers at utility-owned plants are paid. As a result, the new merchant owners of these plants have significantly lower aggregate payroll expenses. Decomposing the effect into a merchant effect and a divestiture effect, I find that merchant ownership is the primary driver of these results. As documented in Chapter 1, merchant power plants have lower overall payroll costs than plants owned by utilities. Employment at merchant power plants is characterized by reduced staffing levels but higher average payroll per employee. A hypothesis set forth in that paper is that merchant generators employ fewer workers at the lower end of the wage distribution, resulting in a higher average payroll per employee. The second chapter of this paper examines whether employment at nonutility power plants, that is, those that are either divested or native merchant power plants, is skewed towards more skilled labor. This chapter also considers the extent to which the difference in employment levels is the result of

  19. Deregulation and restructuring of the electric utility industry

    Energy Technology Data Exchange (ETDEWEB)

    Nixon, Hal [Utility Workers Union of America (UWUA), AFL-CIO, (United States)

    1997-12-31

    Federal and state policy makers are currently faced with the rapidly evolving issue of the restructuring and potential deregulation of the electric utility industry, a sector of the economy of huge importance through its sheer size and its impact on the daily life and livelihood of everyone. This paper describes eleven principles that must be adhered to in any restructuring of the electric industry. Adherence to the principle and positions outlined can help assure that the transition in this industry benefits all, not just a few, and that the general health and welfare of the people is protected and enhanced [Espanol] Los legisladores estatales y federales se estan enfrentando con el rapido y envolvente aspecto de la reestructuracion y desregulacion potencial de la industria electrica, un sector de la economia de enorme importancia por su tamano y su impacto en la vida diaria y los medios de vida. En esta ponencia se describen once principios y posiciones que deben ser considerados en cualquier reestructuracion de la industria electrica. El apego a los principios y posiciones comentados puede ayudar a asegurar que la transicion en esta industria deneficie a todos, no solo a unos cuantos, y que la salud general y bienestar de la gente sea protegida y mejorada

  20. Restructuring of LDCs and retail marketing by producers

    International Nuclear Information System (INIS)

    Heath, D.

    1998-01-01

    The restructuring of local distribution companies (LDCs) and retail marketing producers and the challenges facing market participants were discussed. In Canada, LDC operations are regulated by provincial utilities commissions. This presentation focused on Ontario because it has the largest and most active retail gas market in Canada where some significant LDC restructuring initiatives are taking place. The current state of retail gas pricing in Ontario was also reviewed. Consumers Gas or Union Gas are the two utilities that serve most of the 2.2 million natural gas customers in Ontario. Both utilities have fully integrated supply and marketing businesses which include the sale and delivery of natural gas, related products and services. Suncor's recent entry into the retail natural gas market has been successful. Suncor currently has the third largest number of retail customers in Ontario and a significant share of that market. LDCs will become delivery companies who focus on providing reliable and safe distribution of natural gas to all customers and will provide open access to all gas marketers on a non-discriminatory basis. This will result in more sophisticated marketing to retail customers, retail customer contracts will change to fixed term, fixed price agreements, and there will be strong brand identification. Additional opportunities will be created as a result of deregulation of the electricity industry

  1. Electricity distribution industry restructuring, electrification, and competition in South Africa

    International Nuclear Information System (INIS)

    Galen, P.S.

    1997-07-01

    This paper reviews the status of the South African electricity supply industry (ESI) and proposals for reorienting and restructuring it. South Africa has been intensely examining its ESI for more than 4 years in an effort to determine whether and how it should be restructured to best support the country's new economic development and social upliftment goals. The debate has been spirited and inclusive of most ESI stakeholders. The demands on and expectations for the ESI are many and varied. The debate has reflected this diversity of interests and views. In essence, however, there is a consensus on what is expected of the industry, namely, to extend provision of adequate, reliable, and affordable electricity service to all citizens and segments of the economy. This means a large-scale electrification program to reach as many of the nearly 50% of households currently without electricity service as soon as possible, tariff reform to promote equity and efficiency, and the upgrading of service quality now being provided by some of the newly consolidated municipal authorities. The issues involved are how best to achieve these results within the context of the national Reconstruction and Development Program, while accounting for time and resource constraints and balancing the interests of the various parties

  2. Restructuring the Energy System. Report of the Energy Commission

    International Nuclear Information System (INIS)

    1995-01-01

    The commission was instructed to examine the current energy policy programs for restructuring and developing the energy system (i.e. phasing out nuclear power and moving to renewable sources) and to analyze the needs for changes; to propose measures for ensuring an efficient electricity supply under the new conditions of a liberalized electricity market; and to present proposals for a schedule for reorganizing the energy system. The report gives a full picture of the Swedish energy system including supply, consumption, prices, environmental impacts, R and D, and international aspects. The commission concludes that one nuclear power plant can be closed during the 1990's without upsetting the power balances. Phasing out all nuclear plants by year 2010 will create problems with the price levels of electricity supply, and will conflict with the CO 2 reduction objective. The proposals for economic control measures for performing the restructuring include: An environmental bonus (or investment support) for environmentally sound electricity production financed by an electricity tax, a tax on nuclear power increasing with the age of the reactors, a reorganization fund to finance new and environmentally acceptable electricity production. Also, energy research should be allotted greater resources, in particular for new technology for electricity production. The commission points towards the possibilities for reducing energy consumption, and especially electricity consumption. Space heating should gradually move away from electric heating. Examples are given on measures for improving energy efficiency and problems with financing such measures should be studied

  3. Study of the Socratic method during cognitive restructuring.

    Science.gov (United States)

    Froján-Parga, María Xesús; Calero-Elvira, Ana; Montaño-Fidalgo, Montserrat

    2011-01-01

    Cognitive restructuring, in particular in the form of the Socratic method, is widely used by clinicians. However, little research has been published with respect to underlying processes, which has hindered well-accepted explanations of its effectiveness. The aim of this study is to present a new method of analysis of the Socratic method during cognitive restructuring based on the observation of the therapist's verbal behaviour. Using recordings from clinical sessions, 18 sequences were selected in which the Socratic method was applied by six cognitive-behavioural therapists working at a private clinical centre in Madrid. The recordings involved eight patients requiring therapy for various psychological problems. Observations were coded using a category system designed by the authors and that classifies the therapist's verbal behaviour into seven hypothesized functions based on basic behavioural operations. We used the Observer XT software to code the observed sequences. The results are summarized through a preliminary model which considers three different phases of the Socratic method and some functions of the therapist's verbal behaviour in each of these phases: discriminative and reinforcement functions in the starting phase, informative and motivational functions in the course of the debate, and instructional and reinforcement functions in the final phase. We discuss the long-term potential clinical benefits of the current proposal.  Copyright © 2010 John Wiley & Sons, Ltd.

  4. Closing Kynect and Restructuring Medicaid Threaten Kentucky's Health and Economy.

    Science.gov (United States)

    Wright, Charles B; Vanderford, Nathan L

    2017-08-01

    Following passage of the Patient Protection and Affordable Care Act (ACA) in the United States, the Kentucky Health Benefit Exchange, Kynect, began operating in Kentucky in October 2013. Kentucky expanded Medicaid eligibility in January 2014. Together, Kynect and Medicaid expansion provided access to affordable health care coverage to hundreds of thousands of individuals in Kentucky. However, following the Kentucky gubernatorial election in 2015, the newly inaugurated governor moved to dismantle Kynect and restructure the Medicaid expansion, jeopardizing public health gains and the state economy. As the first state to announce both the closure and restructuring of a state health insurance marketplace and Medicaid expansion, Kentucky may serve as a test case for the rest of the nation for reversal of ACA-related health policies. This article describes Kynect and the Kentucky Medicaid expansion and examines the potential short-term and long-term impacts that may occur following changes in state health policy. Furthermore, this article will offer potential strategies to ameliorate the expected negative impacts of disruption of both Kynect and the Medicaid expansion, such as the creation of a new state insurance marketplace under a new governor, the implementation of a private option, and increasing the state minimum wage for workers. Copyright © 2017 by Duke University Press.

  5. Restructuring Teachers' Work-Lives and Knowledge in England and Spain

    Science.gov (United States)

    Muller, Jorg; Norrie, Caroline; Hernandez, Fernando; Goodson, Ivor

    2010-01-01

    This article explores the restructuring of education in England and Spain. Against a presumably homogeneous global streamlining of educational systems according to competition-driven goals, the comparison of teachers' work-lives and professional knowledge evidences a variety of experiences under-represented in discourses on global restructuring.…

  6. Application of Educational Theories in Restructuring an Introductory Course in Renewable Energy Engineering

    Science.gov (United States)

    Kalkani, Efrossini C.; Boussiakou, Iris K.; Boussiakou, Leda G.

    2004-01-01

    The primary objective of this paper is to apply the educational theories of Kolb's experiential learning and Bloom's educational taxonomy in restructuring the course "Renewable energy engineering". The steps of the research procedure investigate the application of learning theories to the restructuring of the course and the introduction of…

  7. Crisis in the Restructuring of China's Vocational Education System, 1980-2010

    Science.gov (United States)

    Luo, Yan

    2013-01-01

    This article examines the origins of China's vocational education system and the restructuring of the system since 1980, finding that this thirty-year systemic restructuring was based on a framework of instrumental rationalism, but did not connect effectively with the building of a modern enterprise system. During this critical period in upgrading…

  8. Industry Restructuring and Job Loss: Helping Older Workers Get Back into Employment. Research Report

    Science.gov (United States)

    Callahan, Victor J.; Bowman, Kaye

    2015-01-01

    Globalisation and increased competition bring with them many benefits for business, consumers and the economy. But they can also result in the restructuring of industries not able to compete with changing economic markets. In the past, Australia has witnessed restructuring in many high-profile businesses, especially those in its manufacturing…

  9. Understanding the Experiences of Relocatees During Forced Relocation in Chinese Urban Restructuring

    NARCIS (Netherlands)

    Li, X.; van Ham, M.; Kleinhans, R.J.

    Despite the massive forced relocation of residents during urban restructuring in China, there are no systematic studies on how residents undergo the process. Most studies concerning urban restructuring in China directly equate forced relocation with displacement, which has a negative connotation.

  10. 77 FR 4927 - Loan Workouts and Nonaccrual Policy, and Regulatory Reporting of Troubled Debt Restructured Loans

    Science.gov (United States)

    2012-02-01

    ... Restructuring'' is as defined in generally accepted accounting principles (GAAP) and means a restructuring in... definition of a TDR under GAAP. In addition, it is important to recognize the Financial Accounting Standards... accepted accounting principles (GAAP) and the Board does not intend through this policy to change the...

  11. The Effects of Rent Restructuring on Social Housing in English Rural Areas

    Science.gov (United States)

    Walker, Bruce

    2004-01-01

    This paper discusses the impact of central government's rent restructuring policy on social housing in rural areas in England. It examines the effect that restructuring will have on the rents set by social landlords in a set of case study areas then considers some of the likely impacts on affordability and on new investment in rural social…

  12. 24 CFR 401.420 - When must the Restructuring Plan require project-based assistance?

    Science.gov (United States)

    2010-04-01

    ... HOUSING ASSISTANCE RESTRUCTURING PROGRAM (MARK-TO-MARKET) Restructuring Plan § 401.420 When must the..., if: (a) The PAE determines there is a market-wide vacancy rate of 6 percent or less; (b) At least 50 percent of the units in the project are occupied by elderly families, disabled families, or elderly and...

  13. Banking the Furnace: Restructuring of the Steel Industry in Eight Countries.

    Science.gov (United States)

    Bain, Trevor

    A study examined how the cross-national differences in the social contract among managers, unions, and government influenced adjustment strategies in steel. The restructuring process in eight major steel-producing countries was studied to determine who bore the costs of restructuring--employers, employees, or government--and which industrial…

  14. Changes in patient safety culture after restructuring of intensive care units: Two cross-sectional studies.

    Science.gov (United States)

    Vifladt, Anne; Simonsen, Bjoerg O; Lydersen, Stian; Farup, Per G

    2016-02-01

    Compare changes in registered nurses' perception of the patient safety culture in restructured and not restructured intensive care units during a four-year period. Two cross-sectional surveys were performed, in 2008/2009 (time 1) and 2012/2013 (time 2). During a period of 0-3 years after time 1, three of six hospitals merged their general and medical intensive care units (restructured). The other hospitals maintained their structure of the intensive care units (not restructured). Intensive care units in hospitals at one Norwegian hospital trust. The safety culture was measured with Hospital Survey on Patient Safety Culture. At times 1 and 2, 217/302 (72%) and 145/289 (50%) registered nurses participated. Restructuring was negatively associated with change in the safety culture, in particular, the dimensions of the safety culture within the unit level. The dimensions most vulnerable for restructuring were manager expectations and actions promoting safety, teamwork within hospital units and staffing. In this study, the restructuring of intensive care units was associated with a negative impact on the safety culture. When restructuring, the management should be particularly aware of changes in the safety culture dimensions manager expectations and actions promoting safety, teamwork within hospital units and staffing. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  15. Competitive cation binding computations of proton balance for reactions of the phosphagen and glycolytic energy systems within skeletal muscle

    Science.gov (United States)

    2017-01-01

    Limited research and data has been published for the H+ coefficients for the metabolites and reactions involved in non-mitochondrial energy metabolism. The purpose of this investigation was to compute the fractional binding of H+, K+, Na+ and Mg2+ to 21 metabolites of skeletal muscle non-mitochondrial energy metabolism, resulting in 104 different metabolite-cation complexes. Fractional binding of H+ to these metabolite-cation complexes were applied to 17 reactions of skeletal muscle non-mitochondrial energy metabolism, and 8 conditions of the glycolytic pathway based on the source of substrate (glycogen vs. glucose), completeness of glycolytic flux, and the end-point of pyruvate vs. lactate. For pH conditions of 6.0 and 7.0, respectively, H+ coefficients (-‘ve values = H+ release) for the creatine kinase, adenylate kinase, AMP deaminase and ATPase reactions were 0.8 and 0.97, -0.13 and -0.02, 1.2 and 1.09, and -0.01 and -0.66, respectively. The glycolytic pathway is net H+ releasing, regardless of lactate production, which consumes 1 H+. For glycolysis fueled by glycogen and ending in either pyruvate or lactate, H+ coefficients for pH 6.0 and 7.0 were -3.97 and -2.01 (pyruvate), and -1.96 and -0.01 (lactate), respectively. When starting with glucose, the same conditions result in H+ coefficients of -3.98 and -2.67, and -1.97 and –0.67, respectively. The most H+ releasing reaction of glycolysis is the glyceraldehyde-3-phosphate dehydrogenase reaction, with H+ coefficients for pH 6.0 and 7.0 of -1.58 and -0.76, respectively. Incomplete flux of substrate through glycolysis would increase net H+ release due to the absence of the pyruvate kinase and lactate dehydrogenase reactions, which collectively result in H+ coefficients for pH 6.0 and 7.0 of 1.35 and 1.88, respectively. The data presented provide an extensive reference source for academics and researchers to accurately profile the balance of protons for all metabolites and reactions of non-mitochondrial energy

  16. Competitive cation binding computations of proton balance for reactions of the phosphagen and glycolytic energy systems within skeletal muscle.

    Science.gov (United States)

    Robergs, Robert Andrew

    2017-01-01

    Limited research and data has been published for the H+ coefficients for the metabolites and reactions involved in non-mitochondrial energy metabolism. The purpose of this investigation was to compute the fractional binding of H+, K+, Na+ and Mg2+ to 21 metabolites of skeletal muscle non-mitochondrial energy metabolism, resulting in 104 different metabolite-cation complexes. Fractional binding of H+ to these metabolite-cation complexes were applied to 17 reactions of skeletal muscle non-mitochondrial energy metabolism, and 8 conditions of the glycolytic pathway based on the source of substrate (glycogen vs. glucose), completeness of glycolytic flux, and the end-point of pyruvate vs. lactate. For pH conditions of 6.0 and 7.0, respectively, H+ coefficients (-'ve values = H+ release) for the creatine kinase, adenylate kinase, AMP deaminase and ATPase reactions were 0.8 and 0.97, -0.13 and -0.02, 1.2 and 1.09, and -0.01 and -0.66, respectively. The glycolytic pathway is net H+ releasing, regardless of lactate production, which consumes 1 H+. For glycolysis fueled by glycogen and ending in either pyruvate or lactate, H+ coefficients for pH 6.0 and 7.0 were -3.97 and -2.01 (pyruvate), and -1.96 and -0.01 (lactate), respectively. When starting with glucose, the same conditions result in H+ coefficients of -3.98 and -2.67, and -1.97 and -0.67, respectively. The most H+ releasing reaction of glycolysis is the glyceraldehyde-3-phosphate dehydrogenase reaction, with H+ coefficients for pH 6.0 and 7.0 of -1.58 and -0.76, respectively. Incomplete flux of substrate through glycolysis would increase net H+ release due to the absence of the pyruvate kinase and lactate dehydrogenase reactions, which collectively result in H+ coefficients for pH 6.0 and 7.0 of 1.35 and 1.88, respectively. The data presented provide an extensive reference source for academics and researchers to accurately profile the balance of protons for all metabolites and reactions of non-mitochondrial energy

  17. Competitive cation binding computations of proton balance for reactions of the phosphagen and glycolytic energy systems within skeletal muscle.

    Directory of Open Access Journals (Sweden)

    Robert Andrew Robergs

    Full Text Available Limited research and data has been published for the H+ coefficients for the metabolites and reactions involved in non-mitochondrial energy metabolism. The purpose of this investigation was to compute the fractional binding of H+, K+, Na+ and Mg2+ to 21 metabolites of skeletal muscle non-mitochondrial energy metabolism, resulting in 104 different metabolite-cation complexes. Fractional binding of H+ to these metabolite-cation complexes were applied to 17 reactions of skeletal muscle non-mitochondrial energy metabolism, and 8 conditions of the glycolytic pathway based on the source of substrate (glycogen vs. glucose, completeness of glycolytic flux, and the end-point of pyruvate vs. lactate. For pH conditions of 6.0 and 7.0, respectively, H+ coefficients (-'ve values = H+ release for the creatine kinase, adenylate kinase, AMP deaminase and ATPase reactions were 0.8 and 0.97, -0.13 and -0.02, 1.2 and 1.09, and -0.01 and -0.66, respectively. The glycolytic pathway is net H+ releasing, regardless of lactate production, which consumes 1 H+. For glycolysis fueled by glycogen and ending in either pyruvate or lactate, H+ coefficients for pH 6.0 and 7.0 were -3.97 and -2.01 (pyruvate, and -1.96 and -0.01 (lactate, respectively. When starting with glucose, the same conditions result in H+ coefficients of -3.98 and -2.67, and -1.97 and -0.67, respectively. The most H+ releasing reaction of glycolysis is the glyceraldehyde-3-phosphate dehydrogenase reaction, with H+ coefficients for pH 6.0 and 7.0 of -1.58 and -0.76, respectively. Incomplete flux of substrate through glycolysis would increase net H+ release due to the absence of the pyruvate kinase and lactate dehydrogenase reactions, which collectively result in H+ coefficients for pH 6.0 and 7.0 of 1.35 and 1.88, respectively. The data presented provide an extensive reference source for academics and researchers to accurately profile the balance of protons for all metabolites and reactions of non

  18. Effectiveness of cognitive restructuring technique to reduce academic pracrastination of vocational high school students

    Directory of Open Access Journals (Sweden)

    Wahyu Nanda Eka Saputra

    2017-03-01

    Full Text Available Academic procrastination is a serious problem among student. Academic procrastination refers to delaying in doing assignments and preparing for examinations until the last period of examination time and submission date of assignments. To solve this problem we could use cognitive restructuring technique. The purposes of this research are to gain the description of academic procrastination decrease by implementing the cognitive restructuring technique. This research used a single subject research as the research design. The type of the single subject research which used in this research is multiple baselines. The result of this research shows that academic procrastination had significant decrease by implementation the cognitive restructuring technique. This research gives advice for two parts. First, it will be better if counselor implement the cognitive restructuring technique to decreasing academic procrastination. Second, it will be better if stakeholder of school gives support to counselor for implementation the cognitive restructuring technique to decrease academic procrastination.

  19. Exposure to Glycolytic Carbon Sources Reveals a Novel Layer of Regulation for the MalT Regulon

    Directory of Open Access Journals (Sweden)

    Sylvia A. Reimann

    2011-01-01

    Full Text Available Bacteria adapt to changing environments by means of tightly coordinated regulatory circuits. The use of synthetic lethality, a genetic phenomenon in which the combination of two nonlethal mutations causes cell death, facilitates identification and study of such circuitry. In this study, we show that the E. coli ompR malTcon double mutant exhibits a synthetic lethal phenotype that is environmentally conditional. MalTcon, the constitutively active form of the maltose system regulator MalT, causes elevated expression of the outer membrane porin LamB, which leads to death in the absence of the osmoregulator OmpR. However, the presence and metabolism of glycolytic carbon sources, such as sorbitol, promotes viability and unveils a novel layer of regulation within the complex circuitry that controls maltose transport and metabolism.

  20. Twofold reduction of phosphofructokinase activity in Lactococcus lactis results in strong decreases in growth rate and in glycolytic flux

    DEFF Research Database (Denmark)

    Andersen, Heidi Winterberg; Solem, Christian; Hammer, Karin

    2001-01-01

    reduced. Surprisingly, the mutants still showed homolactic fermentation, which indicated that the limitation was different from standard glucose-limited conditions, One explanation could be that the reduced activity of phosphofructokinase resulted in the accumulation of sugar-phosphates. Indeed, when one...... kinase and lactate dehydrogenase remained closer to the wild-type level. In defined medium supplemented with glucose, the growth rate of the mutants was reduced to 57 to 70% of wild-type levels and the glycolytic flux was reduced to 62 to 76% of wild-type levels. In complex medium growth was even further...... of the mutants was starved for glucose in glucose-limited chemostat, the growth rate could gradually be increased to 195% of the growth fate observed in glucose-saturated batch culture, suggesting that phosphofructokinase does affect the concentration of upstream metabolites. The pools of glucose-6- phosphate...

  1. Phosphatase control of 4E-BP1 phosphorylation state is central for glycolytic regulation of retinal protein synthesis.

    Science.gov (United States)

    Gardner, Thomas W; Abcouwer, Steven F; Losiewicz, Mandy K; Fort, Patrice E

    2015-09-15

    Control of protein synthesis in insulin-responsive tissues has been well characterized, but relatively little is known about how this process is regulated in nervous tissues. The retina exhibits a relatively high protein synthesis rate, coinciding with high basal Akt and metabolic activities, with the majority of retinal ATP being derived from aerobic glycolysis. We examined the dependency of retinal protein synthesis on the Akt-mTOR signaling and glycolysis using ex vivo rat retinas. Akt inhibitors significantly reduced retinal protein synthesis but did not affect glycolytic lactate production. Surprisingly, the glycolytic inhibitor 2-deoxyglucose (2-DG) markedly inhibited Akt1 and Akt3 activities, as well as protein synthesis. The effects of 2-DG, and 2-fluorodeoxyglucose (2-FDG) on retinal protein synthesis correlated with inhibition of lactate production and diminished ATP content, with all these effects reversed by provision of d-mannose. 2-DG treatment was not associated with increased AMPK, eEF2, or eIF2α phosphorylation; instead, it caused rapid dephosphorylation of 4E-BP1. 2-DG reduced total mTOR activity by 25%, but surprisingly, it did not reduce mTORC1 activity, as indicated by unaltered raptor-associated mTOR autophosphorylation and ribosomal protein S6 phosphorylation. Dephosphorylation of 4E-BP1 was largely prevented by inhibition of PP1/PP2A phosphatases with okadaic acid and calyculin A, and inhibition of PPM1 phosphatases with cadmium. Thus, inhibition of retinal glycolysis diminished Akt and protein synthesis coinciding with accelerated dephosphorylation of 4E-BP1 independently of mTORC1. These results demonstrate a novel mechanism regulating protein synthesis in the retina involving an mTORC1-independent and phosphatase-dependent regulation of 4E-BP1. Copyright © 2015 the American Physiological Society.

  2. An operon encoding three glycolytic enzymes in Lactobacillus delbrueckii subsp. bulgaricus: glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase.

    Science.gov (United States)

    Branny, P; de la Torre, F; Garel, J R

    1998-04-01

    The structural genes gap, pgk and tpi encoding three glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 3-phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI), respectively, have been cloned and sequenced from Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The genes were isolated after screening genomic sublibraries with specific gap and pgk probes obtained by PCR amplification of chromosomal DNA with degenerate primers corresponding to amino acid sequences highly conserved in GAPDHs and PGKs. Nucleotide sequencing revealed that the three genes were organized in the order gap-pgk-tpi. The translation start codons of the three genes were identified by alignment of the N-terminal sequences. These genes predicted polypeptide chains of 338, 403 and 252 amino acids for GAPDH, PGK and TPI, respectively, and they were separated by 96 bp between gap and pgk, and by only 18 bp between pgk and tpi. The codon usage in gap, pgk, tpi and three other glycolytic genes from L. bulgaricus differed, noticeably from that in other chromosomal genes. The site of transcriptional initiation was located by primer extension, and a probable promoter was identified for the gap-pgk-tpi operon. Northern hybridization of total RNA with specific probes showed two transcripts, an mRNA of 1.4 kb corresponding to the gap gene, and a less abundant mRNA of 3.4 kb corresponding to the gap-pgk-tpi cluster. The absence of a visible terminator in the 3'-end of the shorter transcript and the location of this 3'-end inside the pgk gene indicated that this shorter transcript was produced by degradation of the longer one, rather than by an early termination of transcription after the gap gene.

  3. Effects of mouthrinses with chlorhexidine and zinc ions combined with fluoride on the viability and glycolytic activity of dental plaque.

    Science.gov (United States)

    Giertsen, E; Scheie, A A

    1995-10-01

    Inhibition of plaque acidogenicity by a mouthrinse with chlorhexidine (CHX) or zinc ions has been ascribed to a prolonged bacteriostasis due to substantive properties of the agents. The present aim was to study the effects of mouthrinses with CHX and Zn ions combined with fluoride on the viability and glycolytic activity of dental plaque in order to assess the bacteriostatic versus possible bactericidal effects. Following 2 d of plaque accumulation, 4 groups of 10 students rinsed with either 12 mM NaF (F), 0.55 mM CHX diacetate+F (F-CHX), 10 mM Zn acetate+F (F-Zn), or with the three agents in combination (F-CHX-Zn). Plaque samples were collected before and 90 min after mouthrinsing. Thereafter, the in vivo plaque pH response to sucrose was monitored in each student using touch microelectrodes. F-CHX and F-CHX-Zn reduced the in vivo pH fall significantly as compared with F, whereas F-Zn exerted a non-significant inhibition. Pooled pre- and post-rinse plaque samples were used to measure the pH fall during fermentation of [14C]-glucose, and the glycolytic profiles were analyzed by HPLC. Bacterial viability was assessed by counting the colony-forming units (CFU). All mouthrinses except F reduced glucose consumption and acid formation and thus the pH fall. F-CHX reduced the CFU equal to the reduction of glucose consumption, indicating that inhibition of plaque acidogenicity was due to a bactericidal rather than a bacteriostatic effect. F and F-Zn did not reduce the CFU, thus F-Zn decreased glucose metabolism without affecting plaque viability. F-CHX-Zn reduced both the CFU and glucose metabolism of surviving plaque microorganisms.

  4. Glycolytic enzyme activity is essential for domestic cat (Felis catus) and cheetah (Acinonyx jubatus) sperm motility and viability in a sugar-free medium.

    Science.gov (United States)

    Terrell, Kimberly A; Wildt, David E; Anthony, Nicola M; Bavister, Barry D; Leibo, S P; Penfold, Linda M; Marker, Laurie L; Crosier, Adrienne E

    2011-06-01

    We have previously reported a lack of glucose uptake in domestic cat and cheetah spermatozoa, despite observing that these cells produce lactate at rates that correlate positively with sperm function. To elucidate the role of glycolysis in felid sperm energy production, we conducted a comparative study in the domestic cat and cheetah, with the hypothesis that sperm motility and viability are maintained in both species in the absence of glycolytic metabolism and are fueled by endogenous substrates. Washed ejaculates were incubated in chemically defined medium in the presence/absence of glucose and pyruvate. A second set of ejaculates was exposed to a chemical inhibitor of either lactate dehydrogenase (sodium oxamate) or glyceraldehyde-3-phosphate dehydrogenase (alpha-chlorohydrin). Sperm function (motility and acrosomal integrity) and lactate production were assessed, and a subset of spermatozoa was assayed for intracellular glycogen. In both the cat and cheetah, sperm function was maintained without exogenous substrates and following lactate dehydrogenase inhibition. Lactate production occurred in the absence of exogenous hexoses, but only if pyruvate was present. Intracellular glycogen was not detected in spermatozoa from either species. Unexpectedly, glycolytic inhibition by alpha-chlorohydrin resulted in an immediate decline in sperm motility, particularly in the domestic cat. Collectively, our findings reveal an essential role of the glycolytic pathway in felid spermatozoa that is unrelated to hexose metabolism or lactate formation. Instead, glycolytic enzyme activity could be required for the metabolism of endogenous lipid-derived glycerol, with fatty acid oxidation providing the primary energy source in felid spermatozoa.

  5. Shock to the system: Restructuring America's electricity industry

    International Nuclear Information System (INIS)

    Brennan, T.J.; Palmer, K.L.; Kopp, R.J.; Krupnick, A.J.; Stagliano, V.

    1996-01-01

    Recent decades have seen revolutions in communications, finance, and transportation. In a similar way, technological, economic, and political developments are reshaping the US electricity industry. This concise, balanced, and readable primer, produced by a team of economic analysts at Resources for the Future, introduces the concepts, crucial elements, and terminology used in discussions about electricity restructuring. A Shock to the System provides the background necessary to understand the increasing role of competition in electricity markets. The authors present the history of public policy regarding electricity, identify the significant proposals for implementing competition, and examine their potential consequences for utility regulation, industry structure, cost recovery, and the environment. This volume is an instructive guide to the decisions that electricity providers, customers, and policy makers will face, what forms the decisions are likely to take, and what the long-term ramifications may be

  6. Economic restructuring in Eastern Europe and acid rain abatement strategies

    International Nuclear Information System (INIS)

    Amann, Markus; Klaassen, Ger; Schoepp, Wolfgang; Soerensen, Lene; Hordijk, Leen

    1992-01-01

    Acid rain abatement strategies in Europe are currently being discussed in view of the expiration of the Helsinki Protocol on SO 2 emission reduction. The changing energy situation in Eastern European countries is expected to have an influence on the deposition pattern in Europe. The paper presents a consistent energy scenario for Eastern European countries and compares optimal strategies to reduce SO 2 emissions. These strategies are based on runs with the RAINS model in which environmental targets have been set based on critical loads for sulphur. The analysis shows that economic restructuring and efficiency improvements in Eastern European countries, as well as in Western Europe, may result in significantly lower sulphur abatement costs. Potential assistance to Eastern Europe to guarantee desired environmental standards in Western countries should therefore focus not only on providing emission control devices but also on the success of the economic transition process. (author)

  7. Generation Gap Management in Restructured Metallurgical Enterprises in Poland

    Directory of Open Access Journals (Sweden)

    Gajdzik Bożena

    2015-09-01

    Full Text Available This paper concerns the problem of generation gap management resulting from personnel restructuring in foundries in Poland. Structural changes to steelworks led to a sharp decline in employment caused by decreased steelworks production. New hiring was limited as a part of employment rationalisation in the steelworks sector. Such personnel policy eventually led to a generation gap. Ratios between individual age groups of employees are imbalanced at steelworks: the numbers of young personnel are low and of those aged 50+ are high. This research forecasts changes in employment levels for the 2013-2019 period, aimed at closing the generation gap. The paper consists of three parts: (1 a descriptive analysis of labor market demographics in Poland’s steel industry; (2 proposed methodology for HR management model, and (3 econometric models forecasting labor demographics in Poland’s steel industry.

  8. Electricity sector restructuring in India: an environmentally beneficial policy?

    International Nuclear Information System (INIS)

    Perkins, Richard

    2005-01-01

    It has been suggested that reforms to the electricity sector in developing countries encouraging the entry of independent power producers (IPPs) are likely to result in environmental improvements similar to those recently made in a number of developed economies. The present paper evaluates this claim by examining the experience of the Indian power sector. It finds that recent investments by IPPs have reduced the pollution-intensity of electricity generation in the country. Yet they have not brought the significant gains seen in countries such as the UK, nor are they likely to in the foreseeable future. This is largely a product of the nature and context of electricity sector reform in India which is less favourable to environmentally beneficial outcomes. Accordingly, the paper concludes by suggesting that the environmental benefits of restructuring are not automatic, but depend on the existence of an enabling structural, institutional and regulatory framework

  9. Comments on proposed legislation to restructure DOE's uranium enrichment program

    International Nuclear Information System (INIS)

    1991-04-01

    This book focuses on H.R.145, H.R.788, and S.210. Each of the proposed bills would restructure DOE's enrichment program as a government corporation with private financing and would encourage the eventual sale of the corporation to the private sector. In doing so, the bills would, among other things, allow the corporation to set prices to maximize long-term returns; establish a fund to meet the costs of decontamination, decommissioning, and other environmental cleanup costs associated with uranium enrichment activities; transfer interest in DOE's new atomic vapor laser isotope separation (AVLIS) process to the new corporation; and, except for H.R. 145, require the government to pay its share of the costs to clean up mill tailings (mining wastes) generated under government contracts

  10. Health Care Providers' Spirit at Work Within a Restructured Workplace.

    Science.gov (United States)

    Wagner, Joan I J; Brooks, Denise; Urban, Ann-Marie

    2018-01-01

    Spirit at work (SAW) research emerged as a response to care provider determination to maintain a healthy and productive health care work environment, despite restructuring. The aim of this descriptive mixed-methods research is to present the care provider's perceptions of SAW. SAW is a holistic measure of care provider workplace outcomes, defined as the unique experience of individuals who are passionate about and energized by their work. A mixed group of licensed and unlicensed care providers in a continuing care workplace were surveyed. Eighteen Likert-type scale survey questions were further informed by two open-ended questions. Results indicated that unlicensed continuing care providers' perceptions of SAW are lower than licensed care providers. Responses suggest that open discussion between managers and team members, combined with structured workplace interventions, will lead to enhanced SAW and improved patient care. Further research on SAW within the continuing care workplace is required.

  11. The feminization of agriculture? Economic restructuring in rural Latin America

    OpenAIRE

    Deere, Carmen Diana

    2005-01-01

    Les ménages ruraux ne cessent de diversifier leurs stratégies de création de revenus. Cette diversification, l’augmentation du nombre des membres du ménage cherchant un emploi hors de l’exploitation familiale et des femmes rurales travaillant à leur compte ou comme salariées dans l’agriculture et d’autres secteurs sont parmi les principales tendances associées à la crise économique, à la restructuration néolibérale et à la montée des taux de pauvreté dans les zones rurales d’Amérique latine. ...

  12. Energy Prices and Internal Costs in Croatian Energy System Restructuring

    International Nuclear Information System (INIS)

    Potocnik, V. , Magdic, M.

    1995-01-01

    After social and political changes in 1990, energy prices in Croatia have been getting closer to the West European averages, faster than in the most European countries in transition. The energy prices for industry are almost at the West European level, while the energy prices of electricity and natural gas for households and those of the gasoline are well behind. If the population purchasing power parity (PPP) is taken into account, these relations change. While the internalization of external energy costs is under way in the developed world, it has not practically started yet in Croatia. The Croatian energy system restructuring shall require gradual adjustment of energy prices, together with multistage internalization of external energy costs. (author). 6 refs., 3 tabs., 2 figs

  13. Mechanisms and kinetics of precipitate restructuring during irradiation

    International Nuclear Information System (INIS)

    Potter, D.I.; Wiedersich, H.

    1979-01-01

    Irradiations were performed at temperatures from 400 to 700 0 C using 3.0-MeV 58 Ni + ions in order to investigate γ'-precipitate restructuring in two model alloys: Ni-12.8 at% Al and Ni-12.7 at% Si. The precipitates coarsened, with the third power of the average diameter proportional to the damage dose. The temperature dependence of the rate constants for irradiated specimens is described in terms of a modified Lifschitz-Slyozov-Wagner coarsening model that includes radiation-enhanced diffusion. Redistribution of precipitate by enhanced precipitation (Ni-Si) or precipitate dissolution (Ni-Al) at point-defect sinks caused maxima in γ' size with dose. Precipitate redistribution at the free surfaces of irradiated thin foils and at γ/γ' particle interfaces is described. (Auth.)

  14. THE CHALLENGES OF RAISING REVENUES AND RESTRUCTURING SUBSIDIES IN MALAYSIA

    Directory of Open Access Journals (Sweden)

    Suresh Narayanan

    2007-01-01

    Full Text Available Malaysia has run deficit budgets in all but five years since 1970 but past deficits have been managed thanks to substantial oil revenues and high domestic savings. However, the slow growth or decline of several traditional sources of revenue and the rising subsidy bill since 2007 have given pause for reflection on the traditional approach to fiscal management. In this paper, it is argued that fiscal management must not only centre around reducing non-productive expenditures and wasteful leakages but must also confront the problem of reducing and restructuring subsidies, particularly to petrol and petroleum-related products. The global dip in petroleum process has fortuitously provided the respite needed for such an exercise and should not lull policy makersinto complacency. When the economy recovers from the currentdownswing, a solid revenue raising instrument such as the value-addedtax must be introduced in order to wean the economy away from thecurrent over reliance on petroleum-based taxes.

  15. Restructuring of the power sector in Romania - options and perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Leca, A. [University Politechnica, Bucharest (Romania)

    1999-07-01

    Discusses the Romanian energy sector covering the situation under the Communist government and attempts at restructuring since the fall of Communism. Following the fall of Communism 1989, the power sector remained in State ownership and suffered problems such as a lack of a national energy strategy, a weak institutional framework, and the persistence of monopolistic relationships between suppliers and consumers. In 1997 a new government began a programme of energy reform aiming to, for example, improve energy efficiency, set up an institutional framework, reduce environmental impacts and re-evaluate generation capacities of existing plants and those under construction but political problems have slowed the process down. The paper suggests that privatisation is becoming the only solution to problems in the power sector caused by the deterioration of the economic and social situation in Romania. 8 figs., 1 tab.

  16. Restructuring rehabilitation for women: programs for the female drug addict.

    Science.gov (United States)

    Doyle, K M; Quinones, M A; Tracy, G; Young, D; Hughes, J

    1977-12-01

    Two residential therapeutic communities for female addicts--one coeducational and the other all female--encountered serious problems shortly after their formation. The authors found that the male and female staff of the coeducational program had quite different perceptions of the purposes and characteristics of the women's part of the program. In both programs the female staff held such strongly ambivalent feeling toward their roles as women and authority figures that they had difficulty functioning effectively. The authors suggest the need for research that will identify the female addict's special needs, and a restructuring of programs to meet those needs. Further, training programs for female staff must enable them to distinguish between their own ideals and their clients' needs.

  17. Transition-cost issues for a restructuring US electricity industry

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    Utilities regulators can use a variety of approaches to calculate transition costs. We categorized these approaches along three dimensions. The first dimension is the use of administrative vs. market procedures to value the assets in question. Administrative approaches use analytical techniques to estimate transition costs. Market valuation relies on the purchase price of particular assets to determine their market values. The second dimension concerns when the valuation is done, either before or after the restructuring of the electricity industry. The third dimension concerns the level of detail involved in the valuation, what is often called top-down vs. bottom-up valuation. This paper discusses estimation approaches, criteria to assess estimation methods, specific approaches to estimating transition costs, factors that affect transition-cost estimates, strategies to address transition costs, who should pay transition costs, and the integration of cost recovery with competitive markets.

  18. A review of efforts to restructure Texas' electricity market

    International Nuclear Information System (INIS)

    Zarnikau, Jay

    2005-01-01

    Comparisons suggest that Texas has been relatively successful in its efforts to introduce greater competition and customer choice into its unique electricity market (Center for Advancement of Electricity Markets, 2002). Yet, Texas has defied many of the common prescriptions in designing its market. Texas has yet to establish a nodal congestion management system that directly assigns local congestion costs to entities responsible for creating transmission congestion. A liquid power exchange or spot market is absent. Programs designed to encourage demand side responsiveness had a slow start. Market concentration remains high. Market oversight activities are poorly funded. A generation adequacy mechanism or planning reserve margin requirement remains under debate. Has Texas simply been lucky in averting any real disasters? Or are these market features less important than commonly recognized? This article reviews the restructuring initiative and reports some key lessons learned following the first twenty months under the new wholesale market structure and fifteen months of retail competition

  19. A review of efforts to restructure Texas' electricity market

    International Nuclear Information System (INIS)

    Zarnikau, J.

    2005-01-01

    Comparisons suggest that Texas has been relatively successful in its efforts to introduce greater competition and customer choice into its unique electricity market (Center for Advancement of Electricity Markets, 2002). Yet, Texas has defied many of the common prescriptions in designing its market. Texas has yet to establish a nodal congestion management system that directly assigns local congestion costs to entities responsible for creating transmission congestion. A liquid power exchange or spot market is absent. Programs designed to encourage demand side responsiveness had a slow start. Market concentration remains high. Market oversight activities are poorly funded. A generation adequacy mechanism or planning reserve margin requirement remains under debate. Has Texas simply been lucky in averting any real disasters? Or are these market features less important than commonly recognized? This article reviews the restructuring initiative and reports some key lessons learned following the first twenty months under the new wholesale market structure and fifteen months of retail competition. (author)

  20. The organizational restructuring performative act under shareholder value management ideology

    Directory of Open Access Journals (Sweden)

    Patrícia Saltorato

    2017-04-01

    Full Text Available Purpose – This paper’s objective is to present the dynamics involving an organizational restructuring process conducted in a Brazilian subsidiary of a centenary American industrial corporation which claimed to be seeking, by means of this process, to increase the value of the company’s shares, but, which results reveal the distance between the promises and outcomes of this process, unveiling the symbolic-performative nature of such a process. Methodology – The chosen research method was the case study. The data collection involved the participant observation of a company manager (2010-2015; the conduction of non-structured interviews to the company’s managers, directors and CFO (2013-2015; documental research to the company’s internal and public sources (2010-2015; and bibliographical research to scientific papers, business press, market analysts and specialized media (2010-2015. Results – The narratives of success found in the company’s reports portray the restructuring myth as a continuous strategy, through which the company reinforces its efforts in delivering good results to shareholders. And, despite the outcomes of the case study not supporting these narratives, its performative nature legitimize the company’s commitment to the SHV management ideology towards stakeholders of the capital market, even in face of the adoption of antishareholders’ postures, such as anti-takeovers measures, the merging of the CEO and Chairman positions, its CEO perks, etc. Contributions – Paradoxically, although the researched company operates under the cold logic of finances, and under pressure to create shareholder value, it revealed to be subject to the dynamics in which the search for symbolic legitimacy plays a decisive role in maintaining its position within the hierarchy of the socially constructed field of the Management in face of other stakeholders present in the field, reinvigorating the precepts of Bourdieu’s Field Theory and

  1. Long-Term Planning in Restructured Power Systems

    International Nuclear Information System (INIS)

    Botterud, Audun

    2003-01-01

    This thesis describes the development of three decision support models for long-term investment planning in restructured power systems. The model concepts address the changing conditions for the electric power industry, with the introduction of more competitive markets, higher uncertainty and less centralised planning. Under these circumstances there is an emerging need for new planning models, also for analyses of the power system in a long-term perspective. The thesis focuses particularly on how dynamic and stochastic modelling can contribute to the improvement of decision making in a restructured power industry. We argue that the use of such modelling approaches has become more important after the introduction of competitive power markets, due to the participants' increased exposure to price fluctuations and economic risk. Our models can be applied by individual participants in the power system to evaluate investment projects for new power generation capacity. The models can also serve as a decision support tool on a regulatory level, providing analyses of the long-term performance of the power system under different regulations and market designs. In Chapter 1, we give a brief introduction to the ongoing development towards restructuring and liberalisation of the electrical power system. A discussion of the operation and organisation of restructured power systems is also provided. In Chapter 2, we look more specifically at different modelling approaches for expansion planning in electrical power systems. We also discuss how the contributions in this thesis compare to previous work in the field of decision support models for long-term planning in both regulated and competitive power systems. In Chapter 3, we develop a power market simulation model based on system dynamics. The advantages and limitations of using descriptive system dynamics models for long-term planning purposes in this context are also discussed. Chapter 4 is devoted to a novel optimisation

  2. The role of cognitive flexibility in cognitive restructuring skill acquisition among older adults.

    Science.gov (United States)

    Johnco, C; Wuthrich, V M; Rapee, R M

    2013-08-01

    Cognitive flexibility is one aspect of executive functioning that encompasses the ability to produce diverse ideas, consider response alternatives, and modify behaviors to manage changing circumstances. These processes are likely to be important for implementing cognitive restructuring. The present study investigated the impact of cognitive flexibility on older adults' ability to learn cognitive restructuring. Neuropsychological measures of cognitive flexibility were administered to 40 normal community-dwelling older adult volunteers and their ability to implement cognitive restructuring was coded and analyzed. Results indicated that the majority of participants showed good cognitive restructuring skill acquisition with brief training. The multiple regression analysis suggested that those with poorer cognitive flexibility on neuropsychological testing demonstrated poorer quality cognitive restructuring. In particular, perseverative thinking styles appear to negatively impact the ability to learn cognitive restructuring. Further research is needed to clarify whether older adults with poor cognitive flexibility can improve their cognitive restructuring skills with repetition over treatment or whether alternative skills should be considered. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

  3. The restructuring process of the Colombian electricity market; Die Umstrukturierung des kolumbianischen Elektrizitaetsmarktes

    Energy Technology Data Exchange (ETDEWEB)

    Perez Aleman, Y.O. [Pontificia Universidad Javeriana, Santa Fe de Bogota (Colombia). Fakultaet fuer Ingenieurwesen

    1999-06-14

    Colombia is one of the countries where a restructuring process of the electricity industry has taken place in the recent years. The reforms introduced since the beginning of the present decade, have resulted in deep changes into this sector. Such a process seeks to create a competitive sector to manage the profound crisis that has pervaded the electricity supply in Colombia since the 80`s. The adopted model contains several of the elements needed to achieve economic efficiency: Unbundling, competition on generation and on the retail market, open access to electricity networks and regulatory mechanisms in whose activities where a natural monopoly takes place. The aim of this article is to show the Colombian experience in implementing and managing structural changes in the electricity market. (orig.) [Deutsch] Kolumbien ist eines der Laender, in denen in den letzten Jahren eine Umstrukturierung der Elektrizitaetswirtschaft stattgefunden hat. Der Umstrukturierungsprozess zielt darauf ab, eine wettbewerbsfaehigere Elektrizitaetswirtschaft in Kolumbien zu schaffen, um der schweren Krise, in der dieser Wirtschaftszweig seit den achtizger Jahren gesteckt hat, entgegenzuwirken. Das ausgewaehlte Model enthaelt fast alle wichtigen Elemente in bezug auf eine hoehere Effizienz in der Elektrizitaetswirtschaft. Ziel dieses Aufsatzes ist es, die Erfahrungen in Kolumbien bei der Implementierung und Organisation eines liberalisierten und wettbewerbsfaehigen Elektrizitaetsmarktes zu zeigen. (orig.)

  4. European ecological networks and greenways

    DEFF Research Database (Denmark)

    Kristiansen, Ib; Jongman, Rob H.G.; Kulvik, Mart

    2004-01-01

    renewed. Within the framework of nature conservation, the notion of an ecological network has become increasingly important. Throughout Europe, regional and national approaches are in different phases of development, which are all based on recent landscape ecological principles. Ecological networks......In the context of European integration, networks are becoming increasingly important in both social and ecological sense. Since the beginning of the 1990s, societal and scientific exchanges are being restructured as the conceptual approaches towards new nature conservation strategies have been....... This complex interaction between cultural and natural features results in quite different ways for the elaboration of ecological networks and greenways....

  5. Calcium Alginate and Salt/Phosphate as Binding Agents in Restructured Lamb

    OpenAIRE

    Setyawardani, Triana; Raharjo, Sri; sudarmadji, purnama

    2001-01-01

    A study on  restructurization of lamb meat using several binding agents were conducted. Objectives of the study were evaluate  effectivity of Ca–alginate, salt and phosphate as binding agent and their effect on physical properties of the restructured meat stored at -20⁰C for up to 12 weeks. Three binding agents were added to the restructured products, which include NaCl 0.3 %/ NTPP 0.3 %; alginate 0.5 %/Ca-lactate 0.5%; NaCl 0.3 % / NTPP 0.5 %/alginate 0.5% and no binding agent as a control. ...

  6. Clean Restructuring: Design Elements for Low Carbon Wholesale Markets and Beyond

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2016-05-01

    Countries around the world are in various stages of power system reform and restructuring to more effectively meet development goals and decarbonization commitments. Changes in social dynamics, technology, business models, and environmental goals are increasing pressure for countries to consider improvements to their power systems. This brochure overviews the 21st Century Power Partnerships thought leadership report that explores the clean restructuring pathway in depth, envisions an end state, and articulates three main areas of consideration for decision makers embarking on a clean restructuring process. The report also details case studies from Germany, Denmark, and Mexico.

  7. The redefinition of the american and british gas industries: the regulation of the access load to the gas pipelines networks; La restructuration des industries gazieres americaine et britannique: la reglementation de la charge d'acces aux reseaux de gazoducs

    Energy Technology Data Exchange (ETDEWEB)

    David, L

    2000-10-01

    The transport and distribution networks regulation is the main stakes of the regulation reform of the gas industries. This thesis analyzes the models applied in The Usa and in the United Kingdom. The first part deals with the gas industries deregulation in these two countries, the impacts on the economy and the organization of the gas industries. The second part presents a theoretical approach of the regulation applied to the prices of the natural ags transport by gas pipelines. Regulation by the service cost price and by price cap are compared. (A.L.B.)

  8. Caffeine Ingestion Increases Estimated Glycolytic Metabolism during Taekwondo Combat Simulation but Does Not Improve Performance or Parasympathetic Reactivation.

    Directory of Open Access Journals (Sweden)

    João Paulo Lopes-Silva

    Full Text Available The aim of this study was to evaluate the effect of caffeine ingestion on performance and estimated energy system contribution during simulated taekwondo combat and on post-exercise parasympathetic reactivation.Ten taekwondo athletes completed two experimental sessions separated by at least 48 hours. Athletes consumed a capsule containing either caffeine (5 mg∙kg-1 or placebo (cellulose one hour before the combat simulation (3 rounds of 2 min separated by 1 min passive recovery, in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration and rating of perceived exertion were measured before and after each round, while heart rate (HR and the estimated contribution of the oxidative (WAER, ATP-PCr (WPCR, and glycolytic (W[La-] systems were calculated during the combat simulation. Furthermore, parasympathetic reactivation after the combat simulation was evaluated through 1 taking absolute difference between the final HR observed at the end of third round and the HR recorded 60-s after (HRR60s, 2 taking the time constant of HR decay obtained by fitting the 6-min post-exercise HRR into a first-order exponential decay curve (HRRτ, or by 3 analyzing the first 30-s via logarithmic regression analysis (T30.Caffeine ingestion increased estimated glycolytic energy contribution in relation to placebo (12.5 ± 1.7 kJ and 8.9 ± 1.2 kJ, P = 0.04. However, caffeine did not improve performance as measured by attack number (CAF: 26. 7 ± 1.9; PLA: 27.3 ± 2.1, P = 0.48 or attack time (CAF: 33.8 ± 1.9 s; PLA: 36.6 ± 4.5 s, P = 0.58. Similarly, RPE (CAF: 11.7 ± 0.4 a.u.; PLA: 11.5 ± 0.3 a.u., P = 0.62, HR (CAF: 170 ± 3.5 bpm; PLA: 174.2 bpm, P = 0.12, oxidative (CAF: 109.3 ± 4.5 kJ; PLA: 107.9 kJ, P = 0.61 and ATP-PCr energy contributions (CAF: 45.3 ± 3.4 kJ; PLA: 46.8 ± 3.6 kJ, P = 0.72 during the combat simulation were unaffected. Furthermore

  9. Caffeine Ingestion Increases Estimated Glycolytic Metabolism during Taekwondo Combat Simulation but Does Not Improve Performance or Parasympathetic Reactivation.

    Science.gov (United States)

    Lopes-Silva, João Paulo; Silva Santos, Jonatas Ferreira da; Branco, Braulio Henrique Magnani; Abad, César Cavinato Cal; Oliveira, Luana Farias de; Loturco, Irineu; Franchini, Emerson

    2015-01-01

    The aim of this study was to evaluate the effect of caffeine ingestion on performance and estimated energy system contribution during simulated taekwondo combat and on post-exercise parasympathetic reactivation. Ten taekwondo athletes completed two experimental sessions separated by at least 48 hours. Athletes consumed a capsule containing either caffeine (5 mg∙kg-1) or placebo (cellulose) one hour before the combat simulation (3 rounds of 2 min separated by 1 min passive recovery), in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration and rating of perceived exertion were measured before and after each round, while heart rate (HR) and the estimated contribution of the oxidative (WAER), ATP-PCr (WPCR), and glycolytic (W[La-]) systems were calculated during the combat simulation. Furthermore, parasympathetic reactivation after the combat simulation was evaluated through 1) taking absolute difference between the final HR observed at the end of third round and the HR recorded 60-s after (HRR60s), 2) taking the time constant of HR decay obtained by fitting the 6-min post-exercise HRR into a first-order exponential decay curve (HRRτ), or by 3) analyzing the first 30-s via logarithmic regression analysis (T30). Caffeine ingestion increased estimated glycolytic energy contribution in relation to placebo (12.5 ± 1.7 kJ and 8.9 ± 1.2 kJ, P = 0.04). However, caffeine did not improve performance as measured by attack number (CAF: 26. 7 ± 1.9; PLA: 27.3 ± 2.1, P = 0.48) or attack time (CAF: 33.8 ± 1.9 s; PLA: 36.6 ± 4.5 s, P = 0.58). Similarly, RPE (CAF: 11.7 ± 0.4 a.u.; PLA: 11.5 ± 0.3 a.u., P = 0.62), HR (CAF: 170 ± 3.5 bpm; PLA: 174.2 bpm, P = 0.12), oxidative (CAF: 109.3 ± 4.5 kJ; PLA: 107.9 kJ, P = 0.61) and ATP-PCr energy contributions (CAF: 45.3 ± 3.4 kJ; PLA: 46.8 ± 3.6 kJ, P = 0.72) during the combat simulation were unaffected

  10. 24 CFR 401.403 - Rejection of a request for a Restructuring Plan because of actions or omissions of owner or...

    Science.gov (United States)

    2010-04-01

    ... likely to be remedied in a cost-effective manner through the Restructuring Plan. (3) Exception for sale... Restructuring Plan because of actions or omissions of owner or affiliate or project condition. 401.403 Section...-MARKET) Restructuring Plan § 401.403 Rejection of a request for a Restructuring Plan because of actions...

  11. International company restructuring and the effects on high-skilled employees in lead companies

    DEFF Research Database (Denmark)

    Hansen, Nana Wesley

    2016-01-01

    insulated from international restructuring processes. However, effects on wage and working conditions vary dependent on the motivation for restructuring, and as companies learn to take advantage of pools of skilled employees abroad. Continued international restructuring appear to challenge the insulation......International company restructuring involving knowledge intensive jobs causes increasing concern about the effect on the globalization of high-skilled employees. Drawing on case studies in four Danish-based companies, this article argues that high-skilled employees in lead companies are rarely...... of high-skilled workers over time. Further, the article shows that knowledge intensive Companies increasingly apply relational global value chain governance with multidirectional consequences for wage and working conditions among the high-skilled employees....

  12. Cooperatives’ organizational restructuring, strategic attributes and performance: The case of agribusiness cooperatives in Greece

    NARCIS (Netherlands)

    Benos, T.; Kalogeras, N.; Verhees, F.; Sergaki, P.; Pennings, J.M.E.

    2016-01-01

    We develop a classification of traditional versus restructured cooperative organizational attributes based on an inductive approach. Using this classification and integrating concepts from the business literature (i.e., market and brand orientation), we hypothesize three types of relationships: (a)

  13. Defense Industry: Restructuring Costs Paid, Savings Realized, and Means to Ensure Benefits

    National Research Council Canada - National Science Library

    1998-01-01

    ...) the restructuring costs were allowable under the Federal Acquisition Regulation and (2) a DOD contracting officer determined that the business combination was expected to result in overall reduced costs to DOD or preserve a critical defense capability...

  14. Defense Contractor Restructuring; DoD Risks Forfeiting Savings on Fixed-Price Contracts

    National Research Council Canada - National Science Library

    1998-01-01

    ... on fixed-price contracts awarded to companies involved in a business combination between the time a combination is announced and the time that restructuring savings are reflected in the mechanism used...

  15. Energy efficiency and energy service companies in restructured markets: international experience

    International Nuclear Information System (INIS)

    Limaye, Dilip R.

    1999-01-01

    There has been considerable recent activity related to restructuring of the energy supply industries. Restructuring usually involves introducing competition into some elements of the ESI (energy supply industries). In some countries the privatization of the government-owned utility businesses is another key element of industry restructuring. The introduction of competition and privatization in the energy market is likely to realign the roles of the industry players (generators, transmission businesses) distribution 'wires' businesses, and retail suppliers) relative to customers. A key challenge faced by policy-makers is to define the appropriate roles and activities of the public and private sector to assure that economically justifiable levels of sustainable energy services are offered in the marketplace. This paper reviews the mechanisms adopted to implement energy efficiency services in a number of countries that have restructured their energy markets, and develops a framework for assessing, comparing and discussing potential new mechanisms. The paper identifies two types of EE mechanisms. (The author)

  16. Demand participation in the restructured Electric Reliability Council of Texas market

    International Nuclear Information System (INIS)

    Zarnikau, Jay W.

    2010-01-01

    Does an electricity market which has been restructured to foster competition provide greater opportunities for demand response than a traditional regulated utility industry? The experiences of the restructured Electric Reliability Council of Texas (ERCOT) market over the past eight years provide some hope that it is possible to design a competitive market which will properly value and accommodate demand response. While the overall level of demand response in ERCOT is below the levels enjoyed prior to restructuring, there have nonetheless been some promising advances, including the integration of demand-side resources into competitive markets for ancillary services. ERCOT's experiences demonstrate that the degree of demand participation in a restructured market is highly sensitive to the market design. But even in a market which has been deregulated to a large degree, regulatory intervention and special demand-side programs may be needed in order to bolster demand response. (author)

  17. Refining in the 1990's: Restructuring and resurgence

    International Nuclear Information System (INIS)

    Cobb, C.B.

    1994-01-01

    After two years of uncertainty in dealing with the 1990 Clean Air Act Amendments coupled with the shutdown of 5% of total US refining capacity, the industry is now positioning itself for continued operations throughout the remainder of the decade. However, refineries are experiencing a shift in the mode of operations to a period of more restructuring (closings, ventures, alliances, etc.) followed by a resurgence in financial performance. The purpose of this paper is to examine the current industry and highlight the reasons for industry's current plans. The authors also speculate about the strategies companies will choose to better their financial performance. Fundamentally, the characteristics of a mature domestic business remain the driving force that shape decision making. In responding to the maturing of refining, the authors suggest that refiners will change the way they conduct business over the next few years. Building on the theme of the 1993 NPRA paper, strategies will target the domestic side of the business while simultaneously shifting to a global perspective

  18. Restructuring education and its impact on medical education.

    Science.gov (United States)

    Cavazos, L F

    1990-04-01

    The United States has an education deficit that in the long term may be more harmful to the country than the serious budget and trade deficits. U.S. students are far less prepared in mathematics, chemistry, and physics than are their counterparts in Western Europe and Japan. The dropout rate among high school students, and the high and increasing rate of functional illiteracy, blight lives and represent an enormous economic loss to the nation. American education must be restructured at all levels so that local and federal funds can be used flexibly to pursue revised educational goals. Students and their families should be allowed to choose their elementary and secondary schools, and school management should be decentralized and more rooted in the community. The medical profession must become involved in elementary and secondary education, and medical faculty must be involved in their communities. Further, medical faculty must encourage minority students at all educational levels, must recruit minority medical students, and must increase the number of minority faculty members.

  19. US firms still restructuring, cutting costs under oil price uncertainty

    International Nuclear Information System (INIS)

    Koen, A.D.

    1994-01-01

    Despite more than a decade of downsizing, continuing uncertainty in oil markets is forcing US petroleum companies into another round of cutting and restructuring operations. Wellhead gas prices in the US, although still volatile, in the past 2 years have risen to levels adequate to allow profits for most producers in that sector. Higher gas reserves valuations have strengthened producers' overall balance sheets. But the slide in oil prices from the middle of fourth quarter 1993 until the recent upswing the past month has withered producers' financial performances and reserves values. With little prospect of significantly higher oil prices anytime soon, US companies feel they have little choice but to continue pressing cost cutting moves in order to sustain profits in the near term while at the same time earnings a higher return on investment in the long term. Petroleum company executives are overlooking almost no operating or investment strategy thought capable of bolstering the bottom line. Because no two US oil and gas companies are alike, each profit protection plan is a unique mix of similar solutions. Oil and gas production companies most often try to lower operating costs by vigorously selling noncore properties or business units and reducing staff. The paper discusses measures taken by oil and gas companies to lower costs

  20. [Subjectivity, ethics and productivity in post-productive health restructuring].

    Science.gov (United States)

    Gomes, Doris; Ramos, Flávia Regina Souza

    2015-08-01

    The scope of this paper is to analyze the ethical problems generated by the modern stressor pattern of post-transformation productivity in productive restructuring in the health area. It is a qualitative study of the descriptive and exploratory type in which 30 professionals (nurses, doctors and dental surgeons) from a metropolitan region in the South of Brazil were interviewed, all of whom had prior experience in the public and private sectors. The results were analyzed through Discursive Textual Analysis. Capitalization is revealed as a major ethical problem in the series of new issues derived from the productivity-profitability imperative in health, due to the acritical incorporation of ethics that is restricted to the company's interests or to corporate-individual interests. The ethical problem of low professional commitment to the needs of the patient and of the social collective indicates the need to build a new engaged solidarity in order to increase the quality of public healthcare. Productivity targeted at individual and social needs/interests in the area of health requires a new self-managing and collective engagement of the subjects, supported by an institutional and ethical-political effort of group action, cooperation and solidarity.

  1. CHANGE@CERN:Task Force 5 : Restructuring the accelerator sector

    CERN Document Server

    2002-01-01

    The last of our series on the Task Forces. PS accelerator 'We had a clear mandate, which we could approach in a logical way', explains Steve Myers, Head of SL Division and convenor of Task Force 5, 'To avoid duplication of effort in the accelerator sector through a restructuring that would lead to greater efficiency and flexibility and so release resources for the LHC.' The implementation of all their recommendations is already underway, albeit with different time scales. In 2001 the accelerator sector involved more than 900 staff members in three divisions (LHC, PS and SL) and one unit (AC), working in 141 sections within 34 groups. The first step for the Task Force was to identify major activities within the sector and to set up inter-divisional working groups to review these activities (16 in all), identifying the technologies and the numbers of staff associated with each activity. The working groups were also asked to propose ways of grouping the activities into a new more efficient organizational stru...

  2. Hospital restructuring and physician job satisfaction: an empirical study.

    Science.gov (United States)

    Mascia, Daniele; Morandi, Federica; Cicchetti, Americo

    2014-02-01

    The adoption of clinical directorates through the internal reconfiguration of hospital organizations has been one of the most widespread restructuring interventions in many Western European countries. Despite its extensive adoption, a lack of knowledge remains on the analysis of how this reorganization affects professionals' job satisfaction. This paper contributes to the debate on clinical directorates by exploring how the structural characteristics of newly adopted organizational models influence physician's job satisfaction. More than 300 physicians in 18 clinical directorates in the Italian National Health Service were surveyed regarding their overall job satisfaction following the introduction of departmental arrangements. Survey results were then linked to another survey that classified newly adopted models according to the criteria used to merge hospital wards into directorates, by recognizing "Process-integration", "Specialty-integration" and "Mixed-integration" types of directorates. Our findings show that structural aspects of change significantly influenced overall job satisfaction, and that a physician's openness to experience moderated the adoption and implementation of new clinical directorates. Specifically, results demonstrate that physicians with high openness to experience scores were more receptive to the positive impacts of change on overall job satisfaction. Implications for how these findings may facilitate organizational shifts within hospital settings are discussed. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  3. The Potential Economic Impact of Electricity Restructuring in the State of Oklahoma: Phase II Report; FINAL

    International Nuclear Information System (INIS)

    Hadley, SW

    2001-01-01

    In April 1997, the Oklahoma legislature passed a bill to restructure the state's electric industry, requiring that the generation sector be deregulated and allowing retail competition by July 2002. Details of the market structure were to be established later. Senate Bill No.220, introduced in the 2000 legislature, provided additional details on this market, but the bill did not pass. Subsequent discussions have identified the need for an objective analysis of the impact of restructuring on electricity prices and the state's economy, especially considering the experiences of other states following restructuring of their electric systems. Because of the recent experiences of other states undergoing restructuring (e.g., higher prices, greater volatility, lower reliability), concerns have been raised in states currently considering restructuring as to whether their systems are equally vulnerable. Factors such as local generation costs, transmission constraints, market concentration, and market design can all play a role in the success or failure of the market. Energy and ancillary services markets both play a role in having a well-functioning system. Customer responsiveness to market signals can enhance the flexibility of the market. The purpose of this project is to provide a model and process to evaluate the potential price and economic impacts of restructuring the Oklahoma electric industry. The goal is to provide sufficient objective analysis to the Oklahoma legislature that they may make a more informed decision on the timing and details of any future restructuring. It will also serve to inform other stakeholders on the economic issues surrounding restructuring. The project is being conducted in two phases. The Phase I report (Hadley 2001) concentrated on providing an analysis of the Oklahoma system in the near-term, using only present generation and transmission resources. This Phase II report looks further in the future, incorporating the potential of new

  4. Market restructuring and public incentives: the Turkish case of fresh produce marketing cooperatives

    OpenAIRE

    Lemeilleur, Sylvaine; Bignebat, Celine; Codron, Jean Marie; Humboldt University of Berlin

    2006-01-01

    Faced to the fast restructuring agrofood markets in Turkey, the Turkish State set up new institutional devices to support agricultural marketing cooperatives. This article analyses the dynamics of these public incentives towards producers’ organizations on the restructuration of the Turkish fresh fruit and vegetable market. The wholesale market law enacted 1995 established brokers on wholesale market halls who collected an atomized supply and guarantee the access of small producers to large s...

  5. Financial Restructuring in Banking and Corporate Sector Crises: What Policies to Pursue?

    OpenAIRE

    Stijn Claessens; Daniela Klingebiel; Luc Laeven

    2001-01-01

    We review the literature on resolving bank and corporate sector crises to identify government policies that affect the depth of a crisis and the ease and sustainability of recovery, and to analyze their fiscal cost. A consistent framework - including sufficient resources for loss-absorption and private agents facing the right framework of sticks and carrots - is the, although often missing key to successful bank and corporate restructuring. Sustainability of restructuring calls for deeper str...

  6. Preparing nursing students for contemporary practice: restructuring the psychomotor skills laboratory.

    Science.gov (United States)

    Snyder, M D; Fitzloff, B M; Fiedler, R; Lambke, M R

    2000-05-01

    The restructured laboratory experience offered a safe environment that supported student experimentation with psychomotor skills and self-initiated approaches to problem solving. Restructuring psychomotor laboratory experiences with emphasis on communication and conceptualization of principles supported students to begin addressing clinical problems with flexibility, creativity, and the premise for lifelong skill acquisition. Students who have skills that extend beyond technique will inevitably be better prepared to meet the demands of health care systems and patients now and in the future.

  7. Cognitive and Social Structure of the Elite Collaboration Network of Astrophysics: A Case Study on Shifting Network Structures

    Science.gov (United States)

    Heidler, Richard

    2011-01-01

    Scientific collaboration can only be understood along the epistemic and cognitive grounding of scientific disciplines. New scientific discoveries in astrophysics led to a major restructuring of the elite network of astrophysics. To study the interplay of the epistemic grounding and the social network structure of a discipline, a mixed-methods…

  8. Nodal price volatility reduction and reliability enhancement of restructured power systems considering demand-price elasticity

    International Nuclear Information System (INIS)

    Goel, L.; Wu, Qiuwei; Wang, Peng

    2008-01-01

    With the development of restructured power systems, the conventional 'same for all customers' electricity price is getting replaced by nodal prices. Electricity prices will fluctuate with time and nodes. In restructured power systems, electricity demands will interact mutually with prices. Customers may shift some of their electricity consumption from time slots of high electricity prices to those of low electricity prices if there is a commensurate price incentive. The demand side load shift will influence nodal prices in return. This interaction between demand and price can be depicted using demand-price elasticity. This paper proposes an evaluation technique incorporating the impact of the demand-price elasticity on nodal prices, system reliability and nodal reliabilities of restructured power systems. In this technique, demand and price correlations are represented using the demand-price elasticity matrix which consists of self/cross-elasticity coefficients. Nodal prices are determined using optimal power flow (OPF). The OPF and customer damage functions (CDFs) are combined in the proposed reliability evaluation technique to assess the reliability enhancement of restructured power systems considering demand-price elasticity. The IEEE reliability test system (RTS) is simulated to illustrate the developed techniques. The simulation results show that demand-price elasticity reduces the nodal price volatility and improves both the system reliability and nodal reliabilities of restructured power systems. Demand-price elasticity can therefore be utilized as a possible efficient tool to reduce price volatility and to enhance the reliability of restructured power systems. (author)

  9. A 1ST Step Integration of the Restructured MELCOR for the MIDAS Computer Code

    International Nuclear Information System (INIS)

    Park, S. H.; Kim, D. H.; Cho, S. W.

    2006-01-01

    KAERI is developing a localized severe accident code, MIDAS, based on MELCOR. MELCOR uses pointer variables for a fixed-size storage management to save the data. It passes data through two depths, its meaning is not understandable by variable itself. So it is needed to understand the methods for data passing. This method deteriorates the readability, maintainability and portability of the code. As a most important process for a localized severe accident analysis code, it is needed convenient method for data handling. So, it has been used the new features in FORTRAN90 such as a dynamic allocation for the restructuring. The restructuring of the data saving and transferring method of the existing code makes it easy to understand the code. Before an entire restructuring of the code, a restructuring for each package was developed and tested. And then integration of each restructured package was being processed one by one. In this paper, the integrating scope includes the BUR, CF, CVH, DCH, EDF, ESF, MP, SPR, TF and TP packages. As most of them use data within each package and a few packages share data with other packages. The verification was done through comparing the results before and after the restructuring

  10. A Restructuring of the CAV and FDI Package for the MIDAS Computer Code

    International Nuclear Information System (INIS)

    Park, S. H.; Kim, D. H.; Cho, S. W.

    2006-01-01

    As one of the processes for a localized severe accident analysis code, KAERI is developing a severe accident code MIDAS. The MIDAS code is being developed based on MELCOR. The existing data saving method of MELCOR uses pointer variables for a fix-sized storage management, and it deteriorates the readability, maintainability and portability of the code. As a most important process for a localized severe accident analysis code, it is needed convenient method for data handling. So, it has been used the new features in FORTRAN90 such as a dynamic allocation for the restructuring. The restructuring of the data saving and transferring method of the existing code makes it easy to understand the code. Before an entire restructuring of the code, a restructuring template for a simple package was developed and tested. The target for the restructuring in this paper was the CAV and FDI packages. The CAV(cavity) package is responsible for modeling the attack on the basement concrete by hot core materials. The FDI(Fuel Dispersal Interactions) package is responsible for modeling both low and high pressure molten fuel ejection from the RPV into the reactor cavity, control volumes and surfaces. The verification was done through comparing the results before and after the restructuring

  11. THE ROLE OF LOGISTICS IN ENTERPRISE RESTRUCTURING IN THE AUTOMOBILE INDUSTRY

    Directory of Open Access Journals (Sweden)

    F. A. Sabadash

    2016-01-01

    Full Text Available Formation of a market management mechanism in the Russian economy objectively requires strengthening the role of logistics in general and in the restructuring of the automotive industry in Russia in particular. In the article the main reasons that caused such an interest in the analysis is the automotive industry.Methodology. The methodological basis of this article are comparative, economic and statistical analysis methods.The purpose of this article is to reveal the key theoretical and practical issues of the role of logistics in the restructuring of the automotive industry companies, taking into account global transformations occurring in the world economy. This has allowed to specify the main issues associated with the use of logistics in the restructuring of the automotive industry in Russia.Conclusions. The following conclusions were obtained: research in the field of restructuring of the automotive industry enterprises are considered in the most general terms as "any changes to the strategic nature of the enterprise"; The second approach – the authors in their definitions focus on the restructuring of its individual aspects – goals, directions and forms of restructuring.

  12. Preservation of high glycolytic phenotype by establishing new acute lymphoblastic leukemia cell lines at physiologic oxygen concentration

    Energy Technology Data Exchange (ETDEWEB)

    Sheard, Michael A., E-mail: msheard@chla.usc.edu [Developmental Therapeutics Program, USC-CHLA Institute for Pediatric Clinical Research, Division of Hematology-Oncology, Children' s Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (United States); Ghent, Matthew V., E-mail: mattghent@gmail.com [Department of Pathology, Keck School of Medicine, University of Southern California, Health Sciences Campus, Los Angeles, CA 90089 (United States); Cabral, Daniel J., E-mail: dcabral14@gmail.com [Cancer Center and Departments of Cell Biology & Biochemistry, Pharmacology & Neuroscience, Internal Medicine and Pediatrics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430 (United States); Lee, Joanne C., E-mail: joannebarnhart@gmail.com [Cancer Center and Departments of Cell Biology & Biochemistry, Pharmacology & Neuroscience, Internal Medicine and Pediatrics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430 (United States); Khankaldyyan, Vazgen, E-mail: khangaldian@yahoo.com [Developmental Therapeutics Program, USC-CHLA Institute for Pediatric Clinical Research, Division of Hematology-Oncology, Children' s Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (United States); Ji, Lingyun, E-mail: lingyun.ji@med.usc.edu [Developmental Therapeutics Program, USC-CHLA Institute for Pediatric Clinical Research, Division of Hematology-Oncology, Children' s Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (United States); Wu, Samuel Q., E-mail: swu@chla.usc.edu [Medical Genetics, Children' s Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (United States); Kang, Min H., E-mail: min.kang@ttuhsc.edu [Cancer Center and Departments of Cell Biology & Biochemistry, Pharmacology & Neuroscience, Internal Medicine and Pediatrics, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430 (United States); and others

    2015-05-15

    Cancer cells typically exhibit increased glycolysis and decreased mitochondrial oxidative phosphorylation, and they continue to exhibit some elevation in glycolysis even under aerobic conditions. However, it is unclear whether cancer cell lines employ a high level of glycolysis comparable to that of the original cancers from which they were derived, even if their culture conditions are changed to physiologically relevant oxygen concentrations. From three childhood acute lymphoblastic leukemia (ALL) patients we established three new pairs of cell lines in both atmospheric (20%) and physiologic (bone marrow level, 5%) oxygen concentrations. Cell lines established in 20% oxygen exhibited lower proliferation, survival, expression of glycolysis genes, glucose consumption, and lactate production. Interestingly, the effects of oxygen concentration used during cell line initiation were only partially reversible when established cell cultures were switched from one oxygen concentration to another for eight weeks. These observations indicate that ALL cell lines established at atmospheric oxygen concentration can exhibit relatively low levels of glycolysis and these levels are semi-permanent, suggesting that physiologic oxygen concentrations may be needed from the time of cell line initiation to preserve the high level of glycolysis commonly exhibited by leukemias in vivo. - Highlights: • Establishing new ALL cell lines in 5% oxygen resulted in higher glycolytic expression and function. • Establishing new ALL cell lines in 5% oxygen resulted in higher proliferation and lower cell death. • The divergent metabolic phenotypes selected in 5% and 20% oxygen are semi-permanent.

  13. Studies on cell-free metabolism: ethanol production by a yeast glycolytic system reconstituted from purified enzymes

    Energy Technology Data Exchange (ETDEWEB)

    Welch, P; Scopes, R K

    1985-07-01

    A reconstituted glycolytic system has been established from individually purified enzymes to simulate the conversion of glucose to ethanol plus CO/sub 2/ by yeast. Sustained and extensive conversion occurred provided that input of glucose matched the rate of ATP degradation appropriately. ATPase activity could be replaced by arsenate, which uncoupled ATP synthesis from glycolysis. The mode of uncoupling was investigated, and it was concluded that the artificial intermediate, 1-arseno-3-phosphoglycerate, has a half-life of no more than a few milliseconds. Arsenate at 4 mM concentration could simulate the equivalent of 10 ..mu..mol/ml min. of ATPase activity. The reconstituted enzyme system was capable of totally degrading one M (18% w/v) glucose in 8 hours giving 9% (w/v) ethanol. The levels of metabolites during metabolism were measured to detect rate-limiting steps. The successful operation of the reconstituted enzyme system demonstrates that it is possible to carry out complex chemical transformations with multiple enzyme systems in vitro. 36 references.

  14. Testicular Metabolic Reprogramming in Neonatal Streptozotocin-Induced Type 2 Diabetic Rats Impairs Glycolytic Flux and Promotes Glycogen Synthesis

    Science.gov (United States)

    Rato, L.; Alves, M. G.; Dias, T. R.; Cavaco, J. E.; Oliveira, Pedro F.

    2015-01-01

    Defects in testicular metabolism are directly implicated with male infertility, but most of the mechanisms associated with type 2 diabetes- (T2DM) induced male infertility remain unknown. We aimed to evaluate the effects of T2DM on testicular glucose metabolism by using a neonatal-streptozotocin- (n-STZ) T2DM animal model. Plasma and testicular hormonal levels were evaluated using specific kits. mRNA and protein expression levels were assessed by real-time PCR and Western Blot, respectively. Testicular metabolic profile was assessed by 1H-NMR spectroscopy. T2DM rats showed increased glycemic levels, impaired glucose tolerance and hyperinsulinemia. Both testicular and serum testosterone levels were decreased, whereas those of 17β-estradiol were not altered. Testicular glycolytic flux was not favored in testicles of T2DM rats, since, despite the increased expression of both glucose transporters 1 and 3 and the enzyme phosphofructokinase 1, lactate dehydrogenase activity was severely decreased contributing to lower testicular lactate content. However, T2DM enhanced testicular glycogen accumulation, by modulating the availability of the precursors for its synthesis. T2DM also affected the reproductive sperm parameters. Taken together these results indicate that T2DM is able to reprogram testicular metabolism by enhancing alternative metabolic pathways, particularly glycogen synthesis, and such alterations are associated with impaired sperm parameters. PMID:26064993

  15. Upregulation of glycolytic enzymes, mitochondrial dysfunction and increased cytotoxicity in glial cells treated with Alzheimer's disease plasma.

    Directory of Open Access Journals (Sweden)

    Tharusha Jayasena

    Full Text Available Alzheimer's disease (AD is a neurodegenerative disorder associated with increased oxidative stress and neuroinflammation. Markers of increased protein, lipid and nucleic acid oxidation and reduced activities of antioxidant enzymes have been reported in AD plasma. Amyloid plaques in the AD brain elicit a range of reactive inflammatory responses including complement activation and acute phase reactions, which may also be reflected in plasma. Previous studies have shown that human AD plasma may be cytotoxic to cultured cells. We investigated the effect of pooled plasma (n = 20 each from healthy controls, individuals with amnestic mild cognitive impairment (aMCI and Alzheimer's disease (AD on cultured microglial cells. AD plasma and was found to significantly decrease cell viability and increase glycolytic flux in microglia compared to plasma from healthy controls. This effect was prevented by the heat inactivation of complement. Proteomic methods and isobaric tags (iTRAQ found the expression level of complement and other acute phase proteins to be altered in MCI and AD plasma and an upregulation of key enzymes involved in the glycolysis pathway in cells exposed to AD plasma. Altered expression levels of acute phase reactants in AD plasma may alter the energy metabolism of glia.

  16. Preservation of high glycolytic phenotype by establishing new acute lymphoblastic leukemia cell lines at physiologic oxygen concentration

    International Nuclear Information System (INIS)

    Sheard, Michael A.; Ghent, Matthew V.; Cabral, Daniel J.; Lee, Joanne C.; Khankaldyyan, Vazgen; Ji, Lingyun; Wu, Samuel Q.; Kang, Min H.

    2015-01-01

    Cancer cells typically exhibit increased glycolysis and decreased mitochondrial oxidative phosphorylation, and they continue to exhibit some elevation in glycolysis even under aerobic conditions. However, it is unclear whether cancer cell lines employ a high level of glycolysis comparable to that of the original cancers from which they were derived, even if their culture conditions are changed to physiologically relevant oxygen concentrations. From three childhood acute lymphoblastic leukemia (ALL) patients we established three new pairs of cell lines in both atmospheric (20%) and physiologic (bone marrow level, 5%) oxygen concentrations. Cell lines established in 20% oxygen exhibited lower proliferation, survival, expression of glycolysis genes, glucose consumption, and lactate production. Interestingly, the effects of oxygen concentration used during cell line initiation were only partially reversible when established cell cultures were switched from one oxygen concentration to another for eight weeks. These observations indicate that ALL cell lines established at atmospheric oxygen concentration can exhibit relatively low levels of glycolysis and these levels are semi-permanent, suggesting that physiologic oxygen concentrations may be needed from the time of cell line initiation to preserve the high level of glycolysis commonly exhibited by leukemias in vivo. - Highlights: • Establishing new ALL cell lines in 5% oxygen resulted in higher glycolytic expression and function. • Establishing new ALL cell lines in 5% oxygen resulted in higher proliferation and lower cell death. • The divergent metabolic phenotypes selected in 5% and 20% oxygen are semi-permanent

  17. Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sites.

    OpenAIRE

    Santangelo, G M; Tornow, J

    1990-01-01

    Glycolytic gene expression in Saccharomyces cerevisiae is thought to be activated by the GCR and TUF proteins. We tested the hypothesis that GCR function is mediated by TUF/GRF/RAP binding sites (UASRPG elements). We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption. GCR is not required for TUF/GRF/RAP expression or in vitro DNA-binding activity.

  18. Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene

    Directory of Open Access Journals (Sweden)

    Facincani Agda Paula

    2003-01-01

    Full Text Available The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, followed by cloning and expression studies of the enolase gene and determination of its activity. These studies showed that X. fastidiosa does not use the glycolytic pathway to metabolize carbohydrates, which explains the increased duplication time of this phytopatogen. Recombinant enolase was expressed as inclusion bodies and solubilized with urea (most efficient extractor, Triton X-100, and TCA. Enolase extracted from X. fastidiosa and from chicken muscle and liver is irreversibly inactivated by urea. The purification of enolase was partial and resulted in a low yield. No enzymatic activity was detected for either recombinant and native enolases, aldolase, and glyceraldehyde-3-phosphate dehydrogenase, suggesting that X. fastidiosa uses the Entner-Doudoroff pathway to produce pyruvate. Evidence is presented supporting the idea that the regulation of genes and the presence of isoforms with regulation patterns might make it difficult to understand the metabolism of carbohydrates in X. fastidiosa.

  19. Australian health system restructuring - what problem is being solved?

    Science.gov (United States)

    Dwyer, Judith M

    2004-11-19

    BACKGROUND: In recent years, Australian state and territory governments have reviewed and restructured the health systems they lead and regulate. This paper examines the outcomes of the most recent official published reviews of systems and structures; identifies the common themes; and addresses two questions: what problems are being addressed? And how would we know if the changes were successful? RESULTS: In all the broad, systemic reviews, the main health system problems identified were money, hospital utilisation and a weak primary health care system. The solutions are various, but there is a common trend towards centralisation of governance, often at state health authority level, and stronger accountability measures. Other common themes are hospital substitution (services to avoid the need for admission); calls for cooperation across the Commonwealth:state divide, or for its abolition; and the expected range of current efficiency and effectiveness measures (eg amalgamate pathology and support services) and ideas in good currency (eg call centres). The top-down nature of the public review process is noted, along with the political nature of the immediate catalysts for calling on a review. CONCLUSION: The long-standing tension between the pull to centralisation of authority and the need for innovation in care models is heightened by recent changes, which may be counterproductive in an era dominated by the burden of chronic disease. I argue that the current reforms will not succeed in achieving the stated goals unless they make a difference for people with chronic illness. And if this is correct, the most useful focus for evaluation of the success of the reforms may be their impact on the system's ability to develop and deliver better models of care for this growing group of patients.

  20. Organizational restructuring, government control and loss of legitimacy following an organizational crisis: the case of Israel's nonprofit human services.

    Science.gov (United States)

    Mano, Rita; Rosenberg, Dennis

    2014-01-01

    The study explores organizational restructuring following the occurrence of a crisis. Restructuring activities following an intervention are considered here to be indicators of an organization's loss of legitimacy because they have lost their independent status, a basic characteristic of nonprofit human settings. The study shows that according to the Resource Based View of organization restructuring--experienced as downsizing, neglecting and abandoning of projects--organizations are affected by (a) government intervention in decision making; (b) higher demands for accountability; and (c) higher evaluations of performance gaps. On the basis of the study of a sample of 138 Nonprofit Human Services in Israel, the results show that the higher the level of restructuring, the higher the level of legitimacy. However, organization location in metropolitan areas moderates the link between restructuring and legitimacy loss. We conclude that Israel's nonprofit human services being overly dependent on goverhment funding are more prone to restructuring and losing legitimacy following organizational crisis.

  1. Bank Restructuring in Asia: Crisis management in the aftermath of the Asian financial crisis and prospects for crisis prevention -Malaysia-

    OpenAIRE

    ITO Takatoshi; HASHIMOTO Yuko

    2007-01-01

    This paper analyzes the bank restructuring process in Malaysia from the currency crisis of 1997 to present. Even though the banking sector in Malaysia had relatively lower NPLs compared to other Asian countries, financial sector suffered financial crisis and various problems emerged. This paper covers topics such as setting up financial restructuring agencies, a scheme of capital injection to weak banks, and a corporate restructuring process conducted by the Malaysian government. Plans of Mer...

  2. Future gender relations in global restructuring processes case study evidence from knowledge-intensive, manufacturing and service occupations

    OpenAIRE

    Linda Nierling

    2008-01-01

    Global restructuring processes have not only strong implications for European working and living realities, but also have specific outcomes with regard to gender relations. The following contribution analyses in which way global restructuring shapes current gender relations in order to identify important trends and developments for future gender (in)equalities at the workplace. On the basis of a large qualitative study on global restructuring and impacts on different occupational groups it ar...

  3. GPR55 receptor antagonist decreases glycolytic activity in PANC-1 pancreatic cancer cell line and tumor xenografts.

    Science.gov (United States)

    Bernier, Michel; Catazaro, Jonathan; Singh, Nagendra S; Wnorowski, Artur; Boguszewska-Czubara, Anna; Jozwiak, Krzysztof; Powers, Robert; Wainer, Irving W

    2017-11-15

    The Warburg effect is a predominant metabolic pathway in cancer cells characterized by enhanced glucose uptake and its conversion to l-lactate and is associated with upregulated expression of HIF-1α and activation of the EGFR-MEK-ERK, Wnt-β-catenin, and PI3K-AKT signaling pathways. (R,R')-4'-methoxy-1-naphthylfenoterol ((R,R')-MNF) significantly reduces proliferation, survival, and motility of PANC-1 pancreatic cancer cells through inhibition of the GPR55 receptor. We examined (R,R')-MNF's effect on glycolysis in PANC-1 cells and tumors. Global NMR metabolomics was used to elucidate differences in the metabolome between untreated and (R,R')-MNF-treated cells. LC/MS analysis was used to quantify intracellular concentrations of β-hydroxybutyrate, carnitine, and l-lactate. Changes in target protein expression were determined by Western blot analysis. Data was also obtained from mouse PANC-1 tumor xenografts after administration of (R,R')-MNF. Metabolomics data indicate that (R,R')-MNF altered fatty acid metabolism, energy metabolism, and amino acid metabolism and increased intracellular concentrations of β-hydroxybutyrate and carnitine while reducing l-lactate content. The cellular content of phosphoinositide-dependent kinase-1 and hexokinase 2 was reduced consistent with diminished PI3K-AKT signaling and glucose metabolism. The presence of the GLUT8 transporter was established and found to be attenuated by (R,R')-MNF. Mice treated with (R,R')-MNF had significant accumulation of l-lactate in tumor tissue relative to vehicle-treated mice, together with reduced levels of the selective l-lactate transporter MCT4. Lower intratumoral levels of EGFR, pyruvate kinase M2, β-catenin, hexokinase 2, and p-glycoprotein were also observed. The data suggest that (R,R')-MNF reduces glycolysis in PANC-1 cells and tumors through reduced expression and function at multiple controlling sites in the glycolytic pathway. © 2017 UICC.

  4. Temperature induces significant changes in both glycolytic reserve and mitochondrial spare respiratory capacity in colorectal cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Mitov, Mihail I., E-mail: m.mitov@uky.edu [Markey Cancer Center, University of Kentucky, Lexington, KY 40536 (United States); Harris, Jennifer W. [Department of Surgery, University of Kentucky, Lexington, KY 40506 (United States); Alstott, Michael C.; Zaytseva, Yekaterina Y. [Markey Cancer Center, University of Kentucky, Lexington, KY 40536 (United States); Evers, B. Mark [Markey Cancer Center, University of Kentucky, Lexington, KY 40536 (United States); Department of Surgery, University of Kentucky, Lexington, KY 40506 (United States); Butterfield, D. Allan [Markey Cancer Center, University of Kentucky, Lexington, KY 40536 (United States); Department of Chemistry, University of Kentucky, Lexington, KY 40506 (United States)

    2017-05-15

    Thermotherapy, as a method of treating cancer, has recently attracted considerable attention from basic and clinical investigators. A number of studies and clinical trials have shown that thermotherapy can be successfully used as a therapeutic approach for various cancers. However, the effects of temperature on cancer bioenergetics have not been studied in detail with a real time, microplate based, label-free detection approach. This study investigates how changes in temperature affect the bioenergetics characteristics (mitochondrial function and glycolysis) of three colorectal cancer (CRC) cell lines utilizing the Seahorse XF96 technology. Experiments were performed at 32 °C, 37 °C and 42 °C using assay medium conditions and equipment settings adjusted to produce equal oxygen and pH levels ubiquitously at the beginning of all experiments. The results suggest that temperature significantly changes multiple components of glycolytic and mitochondrial function of all cell lines tested. Under hypothermia conditions (32 °C), the extracellular acidification rates (ECAR) of CRC cells were significantly lower compared to the same basal ECAR levels measured at 37 °C. Mitochondrial stress test for SW480 cells at 37 °C vs 42 °C demonstrated increased proton leak while all other OCR components remained unchanged (similar results were detected also for the patient-derived xenograft cells Pt.93). Interestingly, the FCCP dose response at 37 °C vs 42 °C show significant shifts in profiles, suggesting that single dose FCCP experiments might not be sufficient to characterize the mitochondrial metabolic potential when comparing groups, conditions or treatments. These findings provide valuable insights for the metabolic and bioenergetic changes of CRC cells under hypo- and hyperthermia conditions that could potentially lead to development of better targeted and personalized strategies for patients undergoing combined thermotherapy with chemotherapy.

  5. Temperature induces significant changes in both glycolytic reserve and mitochondrial spare respiratory capacity in colorectal cancer cell lines

    International Nuclear Information System (INIS)

    Mitov, Mihail I.; Harris, Jennifer W.; Alstott, Michael C.; Zaytseva, Yekaterina Y.; Evers, B. Mark; Butterfield, D. Allan

    2017-01-01

    Thermotherapy, as a method of treating cancer, has recently attracted considerable attention from basic and clinical investigators. A number of studies and clinical trials have shown that thermotherapy can be successfully used as a therapeutic approach for various cancers. However, the effects of temperature on cancer bioenergetics have not been studied in detail with a real time, microplate based, label-free detection approach. This study investigates how changes in temperature affect the bioenergetics characteristics (mitochondrial function and glycolysis) of three colorectal cancer (CRC) cell lines utilizing the Seahorse XF96 technology. Experiments were performed at 32 °C, 37 °C and 42 °C using assay medium conditions and equipment settings adjusted to produce equal oxygen and pH levels ubiquitously at the beginning of all experiments. The results suggest that temperature significantly changes multiple components of glycolytic and mitochondrial function of all cell lines tested. Under hypothermia conditions (32 °C), the extracellular acidification rates (ECAR) of CRC cells were significantly lower compared to the same basal ECAR levels measured at 37 °C. Mitochondrial stress test for SW480 cells at 37 °C vs 42 °C demonstrated increased proton leak while all other OCR components remained unchanged (similar results were detected also for the patient-derived xenograft cells Pt.93). Interestingly, the FCCP dose response at 37 °C vs 42 °C show significant shifts in profiles, suggesting that single dose FCCP experiments might not be sufficient to characterize the mitochondrial metabolic potential when comparing groups, conditions or treatments. These findings provide valuable insights for the metabolic and bioenergetic changes of CRC cells under hypo- and hyperthermia conditions that could potentially lead to development of better targeted and personalized strategies for patients undergoing combined thermotherapy with chemotherapy.

  6. Ontario electricity industry restructuring : preliminary asset valuation and calculation of stranded debt

    International Nuclear Information System (INIS)

    1998-01-01

    The rationale for restructuring Ontario's electricity industry was restated. Financial elements of the Government's White Paper on the electrical industry included the following: (1) establishing a level playing field on taxes and regulation, (2) restructuring Ontario Hydro into new companies with clear business mandates, and (3) taking action to put the new companies on solid financial ground. To achieve these objectives requires valuation of the new companies as a key part in the restructuring process. This Ministry of Finance document contains preliminary estimates of the total debt and liabilities of Ontario Hydro ($ 39.1 billion), the value of the new generation and service companies ($ 15.8 billion), and the stranded debt ($ 23.3 billion, less the value of dedicated revenue streams of $ 15.4 billion, equal to the residual stranded debt of $ 7.9 billion). The method by which the stranded debt was calculated is also described. It is stressed that the overriding principles governing the financial restructuring plan are to achieve restructuring without increasing electricity rates, to retain maximum value in the electricity sector until stranded debt is retired, and to recover stranded debt from the electricity sector and not from taxpayers. Ministry advisors indicate that these preliminary valuations would allow the new companies to operate as commercial companies in a competitive market and receive investment grade credit ratings. 44 figs

  7. A restructuring of the CF/EDF packages for the MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S.H.; Kim, K.R.; Kim, D.H.

    2004-01-01

    The CF and EDF packages, which allow the user to define the functions of variables in a database and the usage of an external data file, have been restructured for the MIDAS computer code. MIDAS is being developed as an integrated severe accident analysis code with a user-friendly graphical user interface and a modernized data structure. To restructure the code, the data transferring methods of the current MELCOR code are modified and then partially adopted into the CF/EDF packages. The data structure of the current MELCOR code using FORTRAN77 has a difficulty in grasping the meaning of the variables as pointers are used to define their addresses. New features of FORTRAN90 make it possible to allocate the storage dynamically and to use the user-defined data type without pointers leading to an efficient memory treatment and an easy understanding of the code. Restructuring of the CF/EDF packages addressed in this paper includes a module development and subroutine modification. The verification has been done by comparing the results of the modified code with those of the existing code and the trends are almost the same to each other. Therefore the similar approach could be extended to the entire code package for code restructuring. It is expected that the code restructuring will accelerate the code's domestication thanks to a direct understanding of each variable and an easy implementation of the modified or newly developed models. (author)

  8. Efficiency and environmental impacts of electricity restructuring on coal-fired power plants

    Energy Technology Data Exchange (ETDEWEB)

    Chan, H. Ron [Maryland Univ., College Park, MD (United States). Dept. of Economics; Fell, Harrison [Colorado School of Mines, Golden, CO (United States). Division of Economics and Business; Lange, Ian [Stirling Univ. (United Kingdom). Division of Economics; Li, Shanjun [Cornell Univ., Ithaca, NY (United States). Dyson School of Applied Economics and Management

    2013-03-15

    We investigate the impacts of electricity market restructuring on fuel efficiency, utilization and, new to this area, cost of coal purchases among coal-fired power plants using a panel data set from 1991 to 2005. Our study focuses exclusively on coal-fired power plants and uses panel data covering several years after implementation of restructuring. The estimation compares how investor-owned (IOs) plants in states with restructuring changed their behavior relative to IOs in states without. Our analysis finds that restructuring led to: (1) a two percent improvement in fuel efficiency for IOs, (2) a ten percent decrease in unit cost of heat input, and (3) a lower capacity factor even after adjusting for cross-plant generation re-allocation due to cost reductions. Based on these estimates, back-of-the-envelope calculations find that restructuring has led to about 6.5 million dollars in annual cost savings or nearly 12 percent of operating expenses and up to a 7.6 percent emissions reduction per plant.

  9. Strategic restructuring for effective police system in Nigeria

    Directory of Open Access Journals (Sweden)

    Ann I. Ogbo

    2014-11-01

    Full Text Available The success of a security outfit depends on the strategies and structure of the organisation. The study aims to unravel the possible ways of positioning the Nigerian Police force for effective service delivery through strategic restructuring. Nigerian police was instituted by the colonial authors on the threshold of oppression to achieve subjection and control. Upon this pattern of operation, the Nigerian police force lost the confidence of the public. To position this agency for desired performance, several changes should be made in the strategies and structure of the force, de-emphasizing on the issues that are no longer recent problems and emphasizing on the current bane of the nation, such as corruption and insecurity. This paper adopted the mono-method qualitative approach which made use of secondary sources of data collection. Findings, revealed that the department of the Force that was responsible for information and intelligent gathering, the CID has lied dormant for long a time due to lack of adequate structure as a background that will add value to the department. Furthermore, the force was bedevilled with poor information gathering due to lack of trust and confidence in the police force, the level of motivation was found to be low, as there were no insurance policies for the Force. It is thus obvious to note that the Nigerian police force has suitable strategies that are capable of a sustainable performance, but it is challenged by lack of corresponding structure to work out the strategies. The study proposed that one DIG in addition to the twelve DIGs should be integrated to man a department with the duty of developing and maintaining good relationship with the public, and providing EFCC, ICPC and other crime related agencies with the needed force in discharging their duties. Finally, there is a need for an upward review of the reward and compensation package of the Nigerian Police Force as a way of stepping up on motivation

  10. Load As A Reliability Resource in the Restructured Electricity Market

    Energy Technology Data Exchange (ETDEWEB)

    Kueck, J.D.

    2002-06-10

    Recent electricity price spikes are painful reminders of the value that meaningful demand-side responses could bring to the restructuring US electricity system. Review of the aggregate offers made by suppliers confirms that even a modest increase in demand elasticity could dramatically reduce these extremes in price volatility. There is a strong need for dramatically increased customer participation in these markets to enhance system reliability and reduce price volatility. Indeed, allowing customers to manage their loads in response to system conditions might be thought of as the ultimate reliability resource. Most would agree that meaningful demand-side responses to price are the hallmark of a well-functioning competitive market [1]. Yet, in today's markets for electricity, little or no such response is evident. The reason is simple: customers currently do not experience directly the time-varying costs of their consumption decisions. Consequently, they have no incentive to modify these decisions in ways that might enhance system reliability or improve the efficiency of the markets in which electricity is traded. Increased customer participation is a necessary step in the evolution toward more efficient markets for electricity and ancillary services. This scoping report provides a three-part assessment of the current status of efforts to enhance the ability of customer's load to participate in competitive markets with a specific focus on the role of customer loads in enhancing electricity system reliability. First, this report considers the definitions of electricity-reliability-enhancing ancillary services (Section 2) and a preliminary assessment of the ability of customer's loads to provide these services. Second, is a review a variety of programs in which load has been called on as a system reliability resource (Section 3). These experiences, drawn from both past and current utility and ISO programs, focus on programs triggered by system

  11. The Formulation of Binding Agent Concentration on Raw Restructured Goat Meat Trimming Product

    Directory of Open Access Journals (Sweden)

    Rini Mastuti

    2012-02-01

    Full Text Available The utilization of raw materials and formulation method would affect the quality of the processed meat product. Therefore, a research on the processing method to produce a raw structured product should be conducted. The research was testing a single factor comprises of following parameters: control (without binding agent; 0.3%, 0.5% and 0.7% sodium alginat and 0.3%, 0.5% and 0.7% carragenan, respectively. The results showed that the goat meat trimming could be used as raw materials in the production of the best restructured meat using 0.5% carragenan. This raw restructured meat produced had a loss weight of 1.67%, pH value  7.89, WHC  48.345, and moisture content  75.20%.   Keywords : sodium alginat, carragenan, raw restructured goat meat trimming.

  12. Effect of Chicory Fiber and Smoking on Quality Characteristics of Restructured Sausages

    Science.gov (United States)

    Choi, Hyun-Su; Choi, Hyung-Gyu; Choi, Yeong-Seok; Kim, Jong-Hee; Lee, Ju-Ho; Jung, Eun-Hee; Lee, Sang-Hwa; Choi, Yang-Il

    2016-01-01

    This study was conducted to investigate the effects of chicory fiber for the replacement of fat and smoking on quality characteristics of restructured sausages. Treatments were as follows; Control: Pork backfat 20%, T1: Pork backfat 10% + Chicory fiber 10%, T2: Control + Smoking, T3: T1 + Smoking. The addition of chicory fiber significantly reduced the moisture, fat, hardness and pH values, whereas the smoking treatment increased the fat, redness and pH values of restructured sausages (psausages. As a result, although the addition of chicory fiber decreased the quality characteristics of sausage, smoking treatment improved the reduced quality. Therefore, the chicory fiber and smoking treatment is helpful to develop restructured sausage products with reduced fat and compensated quality. PMID:27499674

  13. Electric power industry restructuring and ROE: The case of Korea Electric Power Corporation

    International Nuclear Information System (INIS)

    Won, Gu-Hwan

    2007-01-01

    Korea's electric power industry was previously run by Korea Electric Power Corporation (KEPCO) in a natural monopoly-type structure. As economy of scale was waning in influence and management effectiveness began to increase in importance, KEPCO was divided into six subsidiaries and faced competition beginning in April 2001. This study evaluates the restructuring process for Korea's power industry and analyzes the financial outcomes based on return on equity (ROE). The results show that the ROE ratio increased. In the analysis period (1993-2004), the financial ratio showed a better performance than that before restructuring. However, productivity ratios such as productivity of capital (or gross value added to total assets) and gross value added to property, plant, and equipment decreased compared to the situation before restructuring. It is believed that the results were due to the increased financial leverage of KEPCO. For this reason, KEPCO should make further efforts to manage its debt in a systematic way

  14. Electrical power industry restructuring in Latin America: towards a new mode of organization

    International Nuclear Information System (INIS)

    De Oliveira, A.; Pinto, H.Q.

    1995-01-01

    At the beginning of the 1990s the restructuring of the industrial organization and the regulatory regimes are probably the most important economic and institutional phenomenon in electricity supply industries Latin America countries. This paper analyses the main characteristics of the restructuring in Chili and Brazil. Two major problems are driving this changes: the financial constraints and economic inefficiencies. Despite institutional barriers to privatization initiatives, the participation of new private companies in electricity supply industrial, specially in generation side, is becoming the common aspect of restructuring. This solution requires new regulatory options and new coordination mechanisms. In this context, the electricity companies may promote innovative strategies and adapt their long-term decisions. (authors). 2 tabs., 30 refs

  15. Restructuring the State Electricity Boards (SEB'S) in India: the case of HSEB

    Energy Technology Data Exchange (ETDEWEB)

    Kanika, T.Bhal [Haryana State Electricity Board, HSEB, Dept. of Management (India); Abhishek, Kalra; Madhuri, Agarwal [Haryana State Electricity Board, HSEB, B. Tech, Mechanical Engineering (India)

    2001-11-01

    In India, power generation and distribution is the responsibility of the different States that have their own electricity boards through which this is done. Of late, in the wake of liberalization, many attempts have been made to reform the power sector, in the light of the poor performance of these boards. The Haryana State Electricity Board (HSEB) is the primary supplier of power in one of the States called Haryana. For quite some years, it was facing problems with revenues, low PLF etc. A review was done and counselling help sought from prominent Consultancy firms. As a result a large scale restructuring was planned. The present paper looks at the attempts of HSEB at restructuring and the consequences of the restructuring efforts. (authors)

  16. Restructuring of workflows to minimise errors via stochastic model checking: An automated evolutionary approach

    International Nuclear Information System (INIS)

    Herbert, L.T.; Hansen, Z.N.L.

    2016-01-01

    This paper presents a framework for the automated restructuring of stochastic workflows to reduce the impact of faults. The framework allows for the modelling of workflows by means of a formalised subset of the BPMN workflow language. We extend this modelling formalism to describe faults and incorporate an intention preserving stochastic semantics able to model both probabilistic- and non-deterministic behaviour. Stochastic model checking techniques are employed to generate the state-space of a given workflow. Possible improvements obtained by restructuring are measured by employing the framework's capacity for tracking real-valued quantities associated with states and transitions of the workflow. The space of possible restructurings of a workflow is explored by means of an evolutionary algorithm, where the goals for improvement are defined in terms of optimising quantities, typically employed to model resources, associated with a workflow. The approach is fully automated and only the modelling of the production workflows, potential faults and the expression of the goals require manual input. We present the design of a software tool implementing this framework and explore the practical utility of this approach through an industrial case study in which the risk of production failures and their impact are reduced by restructuring the workflow. - Highlights: • We present a framework which allows for the automated restructuring of workflows. • This framework seeks to minimise the impact of errors on the workflow. • We illustrate a scalable software implementation of this framework. • We explore the practical utility of this approach through an industry case. • The impact of errors can be substantially reduced by restructuring the workflow.

  17. Restructuring of privatisation? - state petroleum enterprises and the global economic adjustment process

    International Nuclear Information System (INIS)

    Ndi, G.K.; Moller, L.E.

    1994-01-01

    The restructuring, and to a lesser extent the privatisation, of state petroleum enterprises in many oil-producing countries has been seen as a means of addressing the shortcomings of previous state regulation. Recent developments in the petroleum industry within the general context of the current privatisation process are examined in this chapter. Political, economic and social reasons are sought for restructuring being the preferred alternative to the privatisation of state petroleum enterprises in developing countries. The benefits, problems and obstacles to privatisation are analysed. (UK)

  18. Formatting data files for repeated-measures analyses in SPSS: Using the Aggregate and Restructure procedures

    Directory of Open Access Journals (Sweden)

    Gyslain Giguère

    2006-03-01

    Full Text Available In this tutorial, we demonstrate how to use the Aggregate and Restructure procedures available in SPSS (versions 11 and up to prepare data files for repeated-measures analyses. In the first two sections of the tutorial, we briefly describe the Aggregate and Restructure procedures. In the final section, we present an example in which the data from a fictional lexical decision task are prepared for analysis using a mixed-design ANOVA. The tutorial demonstrates that the presented method is the most efficient way to prepare data for repeated-measures analyses in SPSS.

  19. Shell's Role in the Future European Gas Scene - Is a Major Market Restructuring Under Way?

    International Nuclear Information System (INIS)

    Segundo, Karen de

    1999-01-01

    The European gas market is being restructured by a combination of major global forces. Environmental considerations like the Kyoto Agreement favour the use of gas. But in the medium-term, supplies may be limited by low energy prices. The political drive for increased competition has manifested itself as the EU Gas Directive, which came into force in 1998. Shell believes that the European industry must progressively adapt to this restructuring. Flexible mechanisms like tradable emission permits should be used to generate investment capital for new gas supply projects. Moreover, both industry and regulators should take a positive and constructive approach towards increasing competition in the marketplace

  20. Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate.

    Science.gov (United States)

    Nilsson, H; Lindgren, D; Mandahl Forsberg, A; Mulder, H; Axelson, H; Johansson, M E

    2015-01-08

    Changes of cellular metabolism are an integral property of the malignant potential of most cancer cells. Already in the 1930s, Otto Warburg observed that tumor cells preferably utilize glycolysis and lactate fermentation for energy production, rather than the mitochondrial oxidative phosphorylation dominating in normal cells, a phenomenon today known as the Warburg effect. Even though many tumor types display a high degree of aerobic glycolysis, they still retain the activity of other energy-producing metabolic pathways. One exception seems to be the clear cell variant of renal cell carcinoma, ccRCC, where the activity of most other pathways than that of glycolysis has been shown to be reduced. This makes ccRCC a promising candidate for the use of glycolytic inhibitors in treatment of the disease. However, few studies have so far addressed this issue. In this report, we show a strikingly reduced mitochondrial respiratory capacity of primary human ccRCC cells, resulting in enhanced sensitivity to glycolytic inhibition by 3-Bromopyruvate (3BrPA). This effect was largely absent in established ccRCC cell lines, a finding that highlights the importance of using biologically relevant models in the search for new candidate cancer therapies. 3BrPA markedly reduced ATP production in primary ccRCC cells, followed by cell death. Our data suggest that glycolytic inhibitors such as 3BrPA, that has been shown to be well tolerated in vivo, should be further analyzed for the possible development of selective treatment strategies for patients with ccRCC.

  1. Characterization of phosphofructokinase activity in Mycobacterium tuberculosis reveals that a functional glycolytic carbon flow is necessary to limit the accumulation of toxic metabolic intermediates under hypoxia.

    Directory of Open Access Journals (Sweden)

    Wai Yee Phong

    Full Text Available Metabolic versatility has been increasingly recognized as a major virulence mechanism that enables Mycobacterium tuberculosis to persist in many microenvironments encountered in its host. Glucose is one of the most abundant carbon sources that is exploited by many pathogenic bacteria in the human host. M. tuberculosis has an intact glycolytic pathway that is highly conserved in all clinical isolates sequenced to date suggesting that glucose may represent a non-negligible source of carbon and energy for this pathogen in vivo. Fructose-6-phosphate phosphorylation represents the key-committing step in glycolysis and is catalyzed by a phosphofructokinase (PFK activity. Two genes, pfkA and pfkB have been annotated to encode putative PFK in M. tuberculosis. Here, we show that PFKA is the sole PFK enzyme in M. tuberculosis with no functional redundancy with PFKB. PFKA is required for growth on glucose as sole carbon source. In co-metabolism experiments, we report that disruption of the glycolytic pathway at the PFK step results in intracellular accumulation of sugar-phosphates that correlated with significant impairment of the cell viability. Concomitantly, we found that the presence of glucose is highly toxic for the long-term survival of hypoxic non-replicating mycobacteria, suggesting that accumulation of glucose-derived toxic metabolites does occur in the absence of sustained aerobic respiration. The culture medium traditionally used to study the physiology of hypoxic mycobacteria is supplemented with glucose. In this medium, M. tuberculosis can survive for only 7-10 days in a true non-replicating state before death is observed. By omitting glucose in the medium this period could be extended for up to at least 40 days without significant viability loss. Therefore, our study suggests that glycolysis leads to accumulation of glucose-derived toxic metabolites that limits long-term survival of hypoxic mycobacteria. Such toxic effect is exacerbated when

  2. Long term effect of curcumin in regulation of glycolytic pathway and angiogenesis via modulation of stress activated genes in prevention of cancer.

    Directory of Open Access Journals (Sweden)

    Laxmidhar Das

    Full Text Available Oxidative stress, an important factor in modulation of glycolytic pathway and induction of stress activated genes, is further augmented due to reduced antioxidant defense system, which promotes cancer progression via inducing angiogenesis. Curcumin, a naturally occurring chemopreventive phytochemical, is reported to inhibit carcinogenesis in various experimental animal models. However, the underlying mechanism involved in anticarcinogenic action of curcumin due to its long term effect is still to be reported because of its rapid metabolism, although metabolites are accumulated in tissues and remain for a longer time. Therefore, the long term effect of curcumin needs thorough investigation. The present study aimed to analyze the anticarcinogenic action of curcumin in liver, even after withdrawal of treatment in Dalton's lymphoma bearing mice. Oxidative stress observed during lymphoma progression reduced antioxidant enzyme activities, and induced angiogenesis as well as activation of early stress activated genes and glycolytic pathway. Curcumin treatment resulted in activation of antioxidant enzyme super oxide dismutase and down regulation of ROS level as well as activity of ROS producing enzyme NADPH:oxidase, expression of stress activated genes HIF-1α, cMyc and LDH activity towards normal level. Further, it lead to significant inhibition of angiogenesis, observed via MMPs activity, PKCα and VEGF level, as well as by matrigel plug assay. Thus findings of this study conclude that the long term effect of curcumin shows anticarcinogenic potential via induction of antioxidant defense system and inhibition of angiogenesis via down regulation of stress activated genes and glycolytic pathway in liver of lymphoma bearing mice.

  3. LeftyA sensitive cytosolic pH regulation and glycolytic flux in Ishikawa human endometrial cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Salker, Madhuri S.; Zhou, Yuetao; Singh, Yogesh [Department of Physiology, University of Tuebingen, 72076 Tuebingen (Germany); Brosens, Jan [Division of Reproductive Health, Warwick Medical School, Clinical Sciences Research Laboratories, University Hospital, Coventry CV2 2DX (United Kingdom); Lang, Florian, E-mail: florian.lang@uni-tuebingen.de [Department of Physiology, University of Tuebingen, 72076 Tuebingen (Germany)

    2015-05-08

    Objective: LeftyA, a powerful regulator of stemness, embryonic differentiation, and reprogramming of cancer cells, counteracts cell proliferation and tumor growth. Key properties of tumor cells include enhanced glycolytic flux, which is highly sensitive to cytosolic pH and thus requires export of H{sup +} and lactate. H{sup +} extrusion is in part accomplished by Na{sup +}/H{sup +} exchangers, such as NHE1. An effect of LeftyA on transport processes has, however, never been reported. The present study thus explored whether LeftyA modifies regulation of cytosolic pH (pHi) in Ishikawa cells, a well differentiated endometrial carcinoma cell model. Methods: NHE1 transcript levels were determined by qRT-PCR, NHE1 protein abundance quantified by Western blotting, pH{sub i} estimated utilizing (2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein [BCECF] fluorescence, Na{sup +}/H{sup +} exchanger activity from Na{sup +} dependent realkalinization after an ammonium pulse, and lactate concentration in the supernatant utilizing an enzymatic assay and subsequent colorimetry. Results: A 2 h treatment with LeftyA (8 ng/ml) significantly decreased NHE1 transcript levels (by 99.6%), NHE1 protein abundance (by 71%), Na{sup +}/H{sup +} exchanger activity (by 55%), pHi (from 7.22 ± 0.02 to 7.05 ± 0.02), and lactate release (by 41%). Conclusions: LeftyA markedly down-regulates NHE1 expression, Na{sup +}/H{sup +} exchanger activity, pHi, and lactate release in Ishikawa cells. Those effects presumably contribute to cellular reprogramming and growth inhibition. - Highlights: • LeftyA, an inhibitor of tumor growth, reduces Na{sup +}/H{sup +}-exchanger activity by 55%. • LeftyA decreases NHE1 transcripts by 99.6% and NHE1 protein by 71%. • LeftyA decreases cytosolic pH from 7.22 ± 0.02 to 7.05 ± 0.02. • Cytosolic acidification by Lefty A decreases glycolysis by 41%. • Cytosolic acidification by Lefty A compromises energy production of tumor cells.

  4. Retinoblastoma treatment: impact of the glycolytic inhibitor 2-deoxy-d-glucose on molecular genomics expression in LHBETATAG retinal tumors

    Directory of Open Access Journals (Sweden)

    Piña Y

    2012-05-01

    involved in its in vitro and in vivo activity in inhibiting tumor cell growth.Keywords: retinoblastoma, hypoxia, genetic expression, glycolytic inhibitor, 2-DG

  5. Expression profiles of glyceraldehyde-3-phosphate dehydrogenase from Clonorchis sinensis: a glycolytic enzyme with plasminogen binding capacity.

    Science.gov (United States)

    Hu, Yue; Zhang, Erhong; Huang, Lisi; Li, Wenfang; Liang, Pei; Wang, Xiaoyun; Xu, Jin; Huang, Yan; Yu, Xinbing

    2014-12-01

    Globally, 15-20 million people are infected with Clonorchis sinensis (C. sinensis) which results in clonorchiasis. In China, clonorchiasis is considered to be one of the fastest-growing food-borne parasitic diseases. That more key molecules of C. sinensis are characterized will be helpful to understand biology and pathogenesis of the carcinogenic liver fluke. Glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) from many species have functions other than their catalytic role in glycolysis. In the present study, we analyzed the sequence and structure of GAPDH from C. sinensis (CsGAPDH) by using bioinformatics tools and obtained its recombinant protein by prokaryotic expression system, to learn its expression profiles and molecular property. CsGAPDH could bind to human intrahepatic biliary epithelial cell in vivo and in vitro by the method of immunofluorescence assays. CsGAPDH also disturbed in lumen of biliary tract near to the parasite in the liver of infected rat. Western blotting analysis together with immunofluorescence assay indicated that CsGAPDH was a component of excretory/secretory proteins (CsESPs) and a surface-localized protein of C. sinensis. Quantitative real-time PCR (Q-PCR) and Western blotting demonstrated that CsGAPDHs are expressed at the life stages of adult worm, metacercaria, and egg, but the expression levels were different from each other. Recombinant CsGAPDH (rCsGAPDH) was confirmed to have the capacity to catalyze the conversion of glyceraldehyde 3-phosphate to D-glycerate 1,3-bisphosphate which was inhibited by AMP in a dose-dependent manner. In addition, rCsGAPDH was able to interact with human plasminogen in a dose-dependent manner by ELISA. The interaction could be inhibited by lysine. The plasminogen binding capacity of rCsGAPDH along with the distribution of CsGAPDH in vivo and in the liver of C. sinensis-infected rat hinted that surface-localized CsGAPDH might play an important role in host invasion of the worm besides its glycolytic

  6. Negative Effect of Ellagic Acid on Cytosolic pH Regulation and Glycolytic Flux in Human Endometrial Cancer Cells.

    Science.gov (United States)

    Abdelazeem, Khalid N M; Singh, Yogesh; Lang, Florian; Salker, Madhuri S

    2017-01-01

    Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions, which is in part accomplished by Na+/H+ exchangers, such as NHE1. The carrier is sensitive to oxidative stress. Growth of tumor cells could be suppressed by the polyphenol Ellagic acid, which is found in various fruits and vegetables. An effect of Ellagic acid on transport processes has, however, never been reported. The present study thus elucidated an effect of Ellagic acid on cytosolic pH (pHi), NHE1 transcript levels, NHE1 protein abundance, Na+/H+ exchanger activity, and lactate release. Experiments were performed in Ishikawa cells without or with prior Ellagic acid (20 µM) treatment. NHE1 transcript levels were determined by qRT-PCR, NHE1 protein abundance by Western blotting, pHi utilizing (2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein [BCECF] fluorescence, Na+/H+ exchanger activity from Na+ dependent realkalinization after an ammonium pulse, cell volume from forward scatter in flow cytometry, reactive oxygen species (ROS) from 2',7'-dichlorodihydrofluorescein fluorescence, glucose uptake utilizing 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose, and lactate concentration in the supernatant utilizing a colorimetric (570 nm)/ fluorometric enzymatic assay. A 48 hour treatment with Ellagic acid (20 µM) significantly decreased NHE1 transcript levels by 75%, NHE1 protein abundance by 95%, pHi from 7.24 ± 0.01 to 7.02 ± 0.01, Na+/H+ exchanger activity by 77%, forward scatter by 10%, ROS by 82%, glucose uptake by 58%, and lactate release by 15%. Ellagic acid (20µM) markedly down-regulates ROS formation and NHE1 expression leading to decreased Na+/H+ exchanger activity, pHi, glucose uptake and lactate release in endometrial cancer cells. Those effects presumably contribute to reprogramming and growth

  7. Negative Effect of Ellagic Acid on Cytosolic pH Regulation and Glycolytic Flux in Human Endometrial Cancer Cells

    Directory of Open Access Journals (Sweden)

    Khalid N. M. Abdelazeem

    2017-04-01

    Full Text Available Background/Aims: Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions, which is in part accomplished by Na+/H+ exchangers, such as NHE1. The carrier is sensitive to oxidative stress. Growth of tumor cells could be suppressed by the polyphenol Ellagic acid, which is found in various fruits and vegetables. An effect of Ellagic acid on transport processes has, however, never been reported. The present study thus elucidated an effect of Ellagic acid on cytosolic pH (pHi, NHE1 transcript levels, NHE1 protein abundance, Na+/H+ exchanger activity, and lactate release. Methods: Experiments were performed in Ishikawa cells without or with prior Ellagic acid (20 µM treatment. NHE1 transcript levels were determined by qRT-PCR, NHE1 protein abundance by Western blotting, pHi utilizing (2',7'-bis-(2-carboxyethyl-5-(and-6-carboxyfluorescein [BCECF] fluorescence, Na+/H+ exchanger activity from Na+ dependent realkalinization after an ammonium pulse, cell volume from forward scatter in flow cytometry, reactive oxygen species (ROS from 2’,7’-dichlorodihydrofluorescein fluorescence, glucose uptake utilizing 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-ylamino-2-deoxyglucose, and lactate concentration in the supernatant utilizing a colorimetric (570 nm/ fluorometric enzymatic assay. Results: A 48 hour treatment with Ellagic acid (20 µM significantly decreased NHE1 transcript levels by 75%, NHE1 protein abundance by 95%, pHi from 7.24 ± 0.01 to 7.02 ± 0.01, Na+/H+ exchanger activity by 77%, forward scatter by 10%, ROS by 82%, glucose uptake by 58%, and lactate release by 15%. Conclusion: Ellagic acid (20µM markedly down-regulates ROS formation and NHE1 expression leading to decreased Na+/H+ exchanger activity, pHi, glucose uptake and lactate release in endometrial cancer cells. Those

  8. The MMPI-2-Restructured Form and the Standard MMPI-2 Clinical Scales in Relation to DSM-IV

    NARCIS (Netherlands)

    Heijden, P.T. van der; Egger, J.I.M.; Rossi, G.M.P.; Grundel, G.; Derksen, J.J.L.

    2013-01-01

    In a Dutch sample of psychiatric outpatients (N = 94), we linked the Minnesota Multiphasic Personality Inventory–2 (MMPI-2; Butcher et al., 2001) Clinical scales and MMPI-2-Restructured Form (MMPI-2-RF; Ben-Porath & Tellegen, 2008) Higher-Order (H-O) scales, Restructured Clinical (RC) scales and

  9. Restructuring Principal Preparation in Illinois: Perspectives on Implementation Successes, Challenges, and Future Outlook. Policy Research: IERC 2015-3

    Science.gov (United States)

    Klostermann, Brenda K.; Pareja, Amber Stitziel; Hart, Holly; White, Bradford R.; Huynh, Michelle Hanh

    2015-01-01

    In June 2010, the Illinois General Assembly passed Public Act 96-0903, a sweeping restructuring of the preparation of school principals and assistant principals that represented 10 years of effort from a broad coalition of stakeholders. The restructuring in Illinois was part of a movement nationwide to provide stronger training for principals in…

  10. Novel retinoblastoma treatment avoids chemotherapy: the effect of optimally timed combination therapy with angiogenic and glycolytic inhibitors on LHBETATAG retinoblastoma tumors

    Directory of Open Access Journals (Sweden)

    Samuel K Houston

    2011-01-01

    Full Text Available Samuel K Houston1, Yolanda Piña1, Timothy G Murray1, Hinda Boutrid1, Colleen Cebulla2, Amy C Schefler1, Wei Shi1, Magda Celdran1, William Feuer1, Jaime Merchan3, Ted J Lampidis41Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; 2Department of Ophthalmology, The Ohio State University, Columbus, OH, USA; 3Division of Hematology/Oncology, Department of Medicine, 4Department of Cell Biology and Anatomy, University of Miami Miller School of Medicine and Sylvester Comprehensive Cancer Center, Miami, FL, USAPurpose: The purpose of this study was to evaluate the effect of optimally timed combination treatment with angiogenic and glycolytic inhibitors on tumor burden, hypoxia, and angiogenesis in advanced retinoblastoma tumors.Methods: LHBETATAG mice (n = 30 were evaluated. Mice were divided into 5 groups (n = 6 and received injections at 16 weeks of age (advanced tumors with a saline, b anecortave acetate (AA, c 2-deoxyglucose (2-DG, d AA + 2-DG (1 day post-AA treatment, or e AA + 2-DG (1 week post-AA treatment. Eyes were enucleated at 21 weeks and tumor sections were analyzed for hypoxia, angiogenesis, and tumor burden.Results: Eyes treated with 2-DG 1 day post-AA injection showed a 23% (P = 0.03 reduction in tumor burden compared with 2-DG alone and a 61% (P < 0.001 reduction compared with saline-treated eyes. Eyes treated with 2-DG 1 week post-AA injection showed no significant decrease in tumor burden compared with 2-DG alone (P = 0.21 and a 56% (P < 0.001 decrease in comparison with saline-treated eyes. 2-DG significantly reduced the total density of new blood vessels in tumors by 44% compared to saline controls (P < 0.001, but did not affect the density of mature vasculature.Conclusions: Combination therapy with angiogenic and glycolytic inhibitors significantly enhanced tumor control. Synergistic effects were shown to be dependent on the temporal course of treatment

  11. Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sites.

    Science.gov (United States)

    Santangelo, G M; Tornow, J

    1990-01-01

    Glycolytic gene expression in Saccharomyces cerevisiae is thought to be activated by the GCR and TUF proteins. We tested the hypothesis that GCR function is mediated by TUF/GRF/RAP binding sites (UASRPG elements). We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption. GCR is not required for TUF/GRF/RAP expression or in vitro DNA-binding activity. Images PMID:2405258

  12. How to Avoid the Negative Consequences of Restructuring the Network of Rural Schools

    Science.gov (United States)

    Suvorova, Galina

    2004-01-01

    Because of the destruction of the agricultural sector of Russia's economy, there is no demand for workers in the countryside, and, as a consequence, the able-bodied population is leaving the countryside and the birth rate has gone down drastically. These factors have resulted in the liquidation of kindergartens and small-enrollment schools and a…

  13. Mobile telecommunications networks restructuring as a response to a challenging environment

    CERN Document Server

    Curwen, P

    2014-01-01

    During the past decade, no industry has grown faster than that of mobile communications, yet coverage of its operations remains scarce. This state-of-the-art book examines the evolving structure and strategic behaviour of the thirty largest operators in the mobile communications industry.

  14. Survey research on whether taxes affect decisions of Slovak managers on cross-border financial restructuring operations

    Directory of Open Access Journals (Sweden)

    Jana Kubicová

    2017-06-01

    Full Text Available This paper main question is whether decisions of Slovak companies about cross-border financial restructuring are affected by taxes. Using a questionnaire survey we investigated the opinions of Slovak companies’ managers on the selected tax provisions related to cross-border transactions. The aim was to determine whether taxes represent a major obstacle for cross-border financial restructuring. We have extracted the factors by means of exploratory factor analysis, then we have verified the differences in the identified factors between the companies of different size and level of indebtedness, and by employing logistic regression we have investigated the dependence between performing cross-border restructuring and the identified tax factors. We concluded that Slovak companies perform cross-border restructurings that are currently also tax-motivated. The intended changes of tax provisions that facilitate international tax planning will have adverse impact on motivation to perform cross-border financial restructuring operations by Slovak companies.

  15. THE IMPACT OF GLOBALIZATION ON INVESTMENT AND AGRICULTURAL RESTRUCTURING: EVIDENCE FROM POLISH AGRICULTURE

    OpenAIRE

    Swinnen, Johan F.M.; Dries, Liesbeth

    2004-01-01

    We analyze the impact of assistance programs offered by food processors to supplying farms based on an original survey of dairy farms and companies in Poland. The results show that these programs have had a significant impact at the farm level: on on-farm investments; product quality; and farm restructuring.

  16. Transformation of an academic medical center: lessons learned from restructuring and downsizing.

    Science.gov (United States)

    Woodard, B; Fottler, M D; Kilpatrick, A O

    1999-01-01

    This article reviews management literature on health care transformation and describes the processes, including restructuring, job redesign, and downsizing, involved in one academic medical center's experience. The article concludes with lessons learned at each of the stages of the transformation process: planning, implementation, and process continuation. Managerial implications for similar transformation efforts in other health care organizations are suggested.

  17. Restructuring Graduate Engineering Education: The M.Eng. Program at Cornell.

    Science.gov (United States)

    Cady, K. Bingham; And Others

    1988-01-01

    Discusses the restructuring of the graduate program to accommodate emerging fields in engineering. Notes half of the graduate degrees Cornell grants each year are M.Eng. degrees. Offers 12 specialties: aerospace, agriculture, chemical, civil, electrical, mechanical and nuclear engineering; computer science, engineering physics; geological…

  18. PRINCIPAL COURSE OF RESTRUCTURING OF MOTHER AND CHILD HEALTH PROTECTION SERVICE IN MEGAPOLIS

    Directory of Open Access Journals (Sweden)

    Yu.A. Shcherbuk

    2008-01-01

    Full Text Available The results of structural modification in pediatric and obstetric services were described in this work. This modification was directed to the increase of a quality of medical care to women and children and to the realization of priority national project «health».Key words: restructuring, children, pregnant women, infant mortality.

  19. Restructured electric power systems analysis of electricity markets with equilibrium models

    CERN Document Server

    2010-01-01

    Electricity market deregulation is driving the power energy production from a monopolistic structure into a competitive market environment. The development of electricity markets has necessitated the need to analyze market behavior and power. Restructured Electric Power Systems reviews the latest developments in electricity market equilibrium models and discusses the application of such models in the practical analysis and assessment of electricity markets.

  20. 78 FR 41866 - Restructuring of Regulations on the Importation of Plants for Planting

    Science.gov (United States)

    2013-07-12

    ... DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service 7 CFR Parts 319 and 340 [Docket No. APHIS-2008-0011] RIN 0579-AD75 Restructuring of Regulations on the Importation of Plants for Planting AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Proposed rule; reopening of...

  1. Public Tourism Education and Training in Greece: A Study of the Necessity for Educational Restructuring

    Science.gov (United States)

    Diplari, Anna; Dimou, Irini

    2010-01-01

    The authors examine the Greek public tourism education and training system and its structure in order to identify whether there is a need for restructuring and development. To this end, they assess the relationship between the knowledge and skills provided and the requirements of the labour market and the needs of tourism enterprises with regard…

  2. The Netherlands: ERM Comparative Analytical Report on 'Public policy and support for restructuring in SMEs'

    NARCIS (Netherlands)

    van het Kaar, R.H.

    2013-01-01

    Although SMEs get a lot of attention in the Netherlands, there is no specific debate on SMEs and restructuring. A range of public instruments directed at SMEs exists, but their focus is mainly on growth and expansion. Many instruments are fiscal in nature. There are no public or social partner

  3. Susceptibility of the MMPI-2 Clinical, Restructured Clinical (RC), and Content Scales to Overreporting and Underreporting

    Science.gov (United States)

    Sellbom, Martin; Ben-Porath, Yossef S.; Graham, John R.; Arbisi, Paul A.; Bagby, R. Michael

    2005-01-01

    The authors examined and compared the susceptibility of three Minnesota Multiphasic Personality Inventory-2 (MMPI-2) scale sets (Clinical, Restructured Clinical [RC], and Content) to over- and underreporting using five analog samples. Two samples of 85 and 191 undergraduate students, respectively, took the MMPI-2 under underreporting versus…

  4. Diagnostic Construct Validity of MMPI-2 Restructured Form (MMPI-2-RF) Scale Scores

    Science.gov (United States)

    Sellbom, Martin; Bagby, R. Michael; Kushner, Shauna; Quilty, Lena C.; Ayearst, Lindsay E.

    2012-01-01

    In the current investigation, the authors examined the diagnostic construct validity of the "Minnesota Multiphasic Personality Inventory-2-Restructured Form" (MMPI-2-RF) in a patient sample. All participants were diagnosed via the "Structured Clinical Interview" for DSM-IV Axis I Disorders (SCID-I/P). The data set used in this…

  5. The MMPI-2 Restructured Clinical Scales in the Assessment of Posttraumatic Stress Disorder and Comorbid Disorders

    Science.gov (United States)

    Wolf, Erika J.; Miller, Mark W.; Orazem, Robert J.; Weierich, Mariann R.; Castillo, Diane T.; Milford, Jaime; Kaloupek, Danny G.; Keane, Terence M.

    2008-01-01

    This study examined the psychometric properties of the Minnesota Multiphasic Personality Inventory-2 (MMPI-2) Restructured Clinical Scales (RCSs) in individuals with posttraumatic stress disorder (PTSD) receiving clinical services at Department of Veterans Affairs medical centers. Study 1 included 1,098 men who completed the MMPI-2 and were…

  6. RISE AND ACQUISITIONS FINANCIAL POTENTIAL IN THE RESTRUCTURING PROCESS OF CORPORATIONS

    Directory of Open Access Journals (Sweden)

    L. Alekseienko

    2013-03-01

    Full Text Available The basic conceptual foundations of financial potential mergers and acquisitions, as factors of corporations external growth are investigated, existing approaches to the concept of restructuring are generalized, necessary components of the absorption capacity of the corporation are defined.

  7. Restructuring Kindergarten in an Urban School District: The Case of Newark, New Jersey.

    Science.gov (United States)

    Kopacsi, Rosemarie; Hochwald, Eve

    A collaborative project of Bank Street College and the Newark Public Schools, the New Beginnings initiative was designed to bring about progressive restructuring of kindergarten classrooms. This study used a combination of qualitative and quantitative methods to examine the impact of the initiative on curriculum, professional development, and…

  8. Recognition of Debt Restructuring and Resolution Measures under the European Union Regulatory Framework

    NARCIS (Netherlands)

    Arons, T.M.C.

    Under the proposed Bank Recovery and Resolution Directive, member states will be required to provide for bail-in powers to restructure failing financial institutions. At this moment, the Dutch, French, UK and German legislator already provide public authorities with resolution powers. In order to be

  9. Restructuring of endophytic bacterial communities in grapevine yellows-diseased and recovered Vitis vinifera L. plants.

    Science.gov (United States)

    Bulgari, Daniela; Casati, Paola; Crepaldi, Paola; Daffonchio, Daniele; Quaglino, Fabio; Brusetti, Lorenzo; Bianco, Piero Attilio

    2011-07-01

    Length heterogeneity-PCR assays, combined with statistical analyses, highlighted that the endophytic bacterial community associated with healthy grapevines was characterized by a greater diversity than that present in diseased and recovered plants. The findings suggest that phytoplasmas can restructure the bacterial community by selecting endophytic strains that could elicit a plant defense response.

  10. 24 CFR 982.504 - Voucher tenancy: Payment standard for family in restructured subsidized multifamily project.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Voucher tenancy: Payment standard... ASSISTANCE: HOUSING CHOICE VOUCHER PROGRAM Rent and Housing Assistance Payment § 982.504 Voucher tenancy: Payment standard for family in restructured subsidized multifamily project. (a) This section applies to...

  11. Effectiveness of the Sexual Attitude Restructuring Curriculum amongst Taiwanese Graduate Students

    Science.gov (United States)

    Lin, Yi-Li; Lin, Yen-Chin

    2018-01-01

    This article discusses the effectiveness of the Sexual Attitude Restructuring (SAR) curriculum in developing positive sexual attitudes amongst Taiwanese graduate students in human sexuality. Through purposive sampling, 32 graduate students in human sexuality were selected to participate in the study. Before and after providing participants with a…

  12. Electric energy restructuring in the European Union: Integration, subsidiarity and the challenge of harmonization

    International Nuclear Information System (INIS)

    Serralles, Roberto J.

    2006-01-01

    In 1996, the European Union (EU) instituted Directive 96/92/EC that aims to establish a competitive, secure and transparent EU-wide internal electric energy market. The process of electricity market restructuring in the EU is being guided primarily by the principle of subsidiarity. As an organizational concept, the principle of subsidiarity allows Member States to establish their own implementation strategies and methods as a means of achieving the EU-mandated goal of electric energy restructuring. Historically, the structure of the electric energy industry in place among the Member States exhibited widely disparate organizational and functional characteristics with regard to ownership, control and regulation. Under these varied and contrasting political and economic conditions, the goal of a EU-wide harmonized electric energy market becomes very challenging. In this paper, I describe the policy mechanisms and the market conditions mandated by the EU directive aimed at liberalizing the electric energy market. I then assess the role of political culture, historical resource endowment and geographical conditions in the utility restructuring strategies of some key Member States and its effects on the overall goals of a harmonized internal electric energy market. Finally, I argue that European electric energy restructuring must be understood within the context of the political and economic milieu that spawned the individual Member States' electric energy industry

  13. Electric energy restructuring in the European Union: integration, subsidiarity and the challenge of harmonization

    International Nuclear Information System (INIS)

    Serralles, R.J.

    2006-01-01

    In 1996, the European Union (EU) instituted Directive 96/92/EC that aims to establish a competitive, secure and transparent EU-wide internal electric energy market. The process of electricity market restructuring in the EU is being guided primarily by the principle of subsidiarity. As an organizational concept, the principle of subsidiarity allows Member States to establish their own implementation strategies and methods as a means of achieving the EU-mandated goal of electric energy restructuring. Historically, the structure of the electric energy industry in place among the Member States exhibited widely disparate organizational and functional characteristics with regard to ownership, control and regulation. Under these varied and contrasting political and economic conditions, the goal of a EU-wide harmonized electric energy market becomes very challenging. In this paper, I describe the policy mechanisms and the market conditions mandated by the EU directive aimed at liberalizing the electric energy market. I then assess the role of political culture, historical resource endowment and geographical conditions in the utility restructuring strategies of some key Member States and its effects on the overall goals of a harmonized internal electric energy market. Finally, I argue that European electric energy restructuring must be understood within the context of the political and economic milieu that spawned the individual Member States' electric energy industry. (author)

  14. 77 FR 31993 - Loan Workouts and Nonaccrual Policy, and Regulatory Reporting of Troubled Debt Restructured Loans

    Science.gov (United States)

    2012-05-31

    ...) instructions for reporting past due status on TDRs, many FICUs maintain separate, manual delinquency... loan delinquency based on restructured contract terms rather than the original loan terms. To that end... loan on nonaccrual status has no impact on the borrower's contractual obligation to the FICU. c...

  15. Cooperatives’ Organizational Restructuring, Strategic Attributes and Performance: The Case of Agribusiness Cooperatives in Greece

    NARCIS (Netherlands)

    Benos, T.; Kalogeras, N.; Verhees, F.J.H.M.; Sergaki, P.; Pennings, J.M.E.

    2016-01-01

    We develop a classification of traditional vs. restructured cooperative organizational attributes based on an inductive approach. Using this classification and integrating concepts from the business literature (i.e., market and brand orientation), we hypothesize three types of relationships: a) the

  16. The Theoretical Foundations of Strategic Management of Restructuring the Enterprises on the Basis of Potentials

    Directory of Open Access Journals (Sweden)

    Shpak Sergiy O.

    2017-04-01

    Full Text Available The article is aimed at developing an internally consistent system of formal strictly defined concepts of the theory of strategic management of industrial restructuring and substantiating, on this basis, appropriateness of an approach to strategic planning for restructuring, based on the monitoring and management of enterprise potentials. It has been shown that restructuring of the production sphere of industrial enterprise is a process of targeted changes in the structure of enterprise, affecting the material-technical supply, production, and marketing subsystems, which are generally managed at the strategic, tactical and operational levels. A comprehensive characterization of the current and future (of the possible structures of enterprise may be provided by its potentials. The conception of strategic management of enterprises restructuring based on the development of their potentials dictates certain requirements to the composition of tasks to be solved and the methodological tools used for this purpose, among them, priority should be given to the development of potentially promising enterprise structures and the choice of the most promising ones, as well as methods for assessment of potentials.

  17. Local responses to global technological change – Contrasting restructuring practices in two rural communities in Austria.

    NARCIS (Netherlands)

    Fink, M.; Lang, R..; Harms, Rainer

    2013-01-01

    In this article, we investigate into local economic restructuring in rural areas that are affected by disruptive technologies. Drawing on an institutionalist framework we apply systematic theory-informed case study analysis of two rural communities in Austria and identify practices that are crucial

  18. Eight Voices of Empowerment: Student Perspectives in a Restructured Urban Middle School

    Science.gov (United States)

    Horn, Brian R.

    2017-01-01

    This article explores student empowerment in a restructured urban Title I middle school. The study includes data from eight participants in an action research project that involved a critical inquiry unit in an eighth-grade language arts class that asked students, "How are you empowered and disempowered by school?" Findings reveal that…

  19. 24 CFR 401.101 - Which owners are ineligible to request Restructuring Plans?

    Science.gov (United States)

    2010-04-01

    ... RESTRUCTURING PROGRAM (MARK-TO-MARKET) General Provisions; Eligibility § 401.101 Which owners are ineligible to...) An affiliate is debarred or suspended under 2 CFR part 2424; or (2) HUD notifies the owner that HUD... affiliate, and the grounds for the pending action are included in § 401.403(b)(2)(ii). (c) Exception for...

  20. Automated evolutionary restructuring of workflows to minimise errors via stochastic model checking

    DEFF Research Database (Denmark)

    Herbert, Luke Thomas; Hansen, Zaza Nadja Lee; Jacobsen, Peter

    2014-01-01

    This paper presents a framework for the automated restructuring of workflows that allows one to minimise the impact of errors on a production workflow. The framework allows for the modelling of workflows by means of a formalised subset of the Business Process Modelling and Notation (BPMN) language...

  1. Restructuring of workflows to minimise errors via stochastic model checking: An automated evolutionary approach

    DEFF Research Database (Denmark)

    Herbert, Luke Thomas; Hansen, Zaza Nadja Lee

    2016-01-01

    This article presents a framework for the automated restructuring of stochastic workflows to reduce the impact of faults. The framework allows for the modelling of workflows by means of a formalised subset of the BPMN workflow language. We extend this modelling formalism to describe faults...

  2. Value Chain Restructuring, Work Organization and Labour Outcomes in Football Manufacturing in India

    DEFF Research Database (Denmark)

    Khara, Navjote; Lund-Thomsen, Peter

    2012-01-01

    this aim, the article develops a new analytical framework based upon the global value chain approach. Applying this framework to the football manufacturing industry of Jalandhar, India, the article concludes that value chain restructuring produces both new forms of work organization and highly gendered...

  3. Enhancing construction infrastructure delivery through restructuring of the Vuk’uphile Learnership Programme

    CSIR Research Space (South Africa)

    Hauptfleisch, AC

    2014-07-01

    Full Text Available The purpose of the paper is to provide an overview on review and restructuring of a construction contractor learnership programme and the quality management under the infrastructure sector of the government’s Expanded Public Works Programme (EPWP...

  4. Effect of Oyster Shell Calcium Powder on the Quality of Restructured Pork Ham.

    Science.gov (United States)

    Choi, Jung-Seok; Lee, Hyun-Jin; Jin, Sang-Keun; Lee, Hyun-Joo; Choi, Yang-Il

    2014-01-01

    This study was conducted to evaluate the effects of oyster shell calcium powder (OSCP) as a substitute for phosphates in curing agent, on the quality of restructured pork ham. Restructured pork ham was processed under six treatment conditions: T1 (no additives), T2 (0.3% sodium tripolyphosphate), T3 (1.5% NaCl+0.5% whey protein), T4 (1.5% NaCl+0.5% whey protein+0.15% OSCP), T5 (1.5% NaCl+0.5% whey protein+0.3% OSCP), and T6 (1.5% NaCl+0.5% whey protein+0.5% OSCP). Addition of OSCP significantly increased the ash content and pH of restructured pork ham (pham. Addition of OSCP had no effect on Hunter a and b surface color values of restructured pork ham, but did decrease the Hunter L surface color value (pham, compared with the addition of phosphates (pham.

  5. 78 FR 79636 - Restructuring of Regulations on the Importation of Plants for Planting

    Science.gov (United States)

    2013-12-31

    ... in Washington, DC, this 20th day of December 2013. Kevin Shea, Administrator, Animal and Plant Health... DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service 7 CFR Parts 319 and 340 [Docket No. APHIS-2008-0011] RIN 0579-AD75 Restructuring of Regulations on the Importation of Plants for...

  6. International divestment and restructuring decisions (with special reference to the motor industry).

    OpenAIRE

    Casson M

    1986-01-01

    ILO pub. Working paper on specific industry restructuring and plant shutdown in the motor vehicle industry in Western Europe - discusses industrial policy issues, enterprise level decision making, production, management, marketing, and the need for rationalization and economies of scale; includes a case study of Peugeot's acquisition of Chrysler. Bibliography, statistical tables.

  7. Independent Power Producers' view on restructuring in Ontario's electric power sector

    International Nuclear Information System (INIS)

    Brooks, J.

    1996-01-01

    The collective views on electricity industry restructuring of the independent power producers in Ontario were summarized by IPPSO's executive director. The Society is fully in agreement with the MacDonald Committee recommendations to privatize power generation in Ontario, and is equally in favor of competitive restructuring that is now underway in Michigan, New York and Quebec, as well as farther afield in the U.S., the U.K., and elsewhere around the world. IPPSO claims that a competitive generation system comprised of current and future IPPSO members could supply the province's power requirements at a cost 20 per cent lower than the present monopolistic system of Ontario Hydro. Add to that no reduction in services to the consumers, increased revenues in the form of taxes to the province, and the prospect of restructuring becomes far less threatening than first perceived. While fully in agreement with the MacDonald Committee's recommendations, IPPSO is opposed to Ontario Hydro's own restructuring plans. Their objection is based on the assessment that the plan would not result in real competition; in reality, it would allow Hydro even greater freedom to continue investing publicly-guaranteed money on a completely dissimilar basis to its competitors

  8. Detecting comorbid Axis-II status among inpatients using the MMPI-2 Restructured Clinical Scales

    NARCIS (Netherlands)

    Kamphuis, J.H.; Arbisi, P.A.; Ben-Porath, Y.S.; McNulty, J.L.

    2008-01-01

    This study examined the differential diagnostic utility of the MMPI-2 Restructured Clinical Scales (RCS) and Clinical Scales (CS) in detecting a complex multivariate clinical phenomenon: that is, comorbid Axis-II status in two matched samples of inpatients. Psychiatric inpatients diagnosed with

  9. Educational Technology and the Restructuring Movement: Lessons from Research on Computers in Classrooms.

    Science.gov (United States)

    Kell, Diane; And Others

    This paper presents findings from a recently completed study of the use of computers in primary classrooms as one source of evidence concerning the role technology can play in school restructuring efforts. The sites for the study were selected by Apple Computer, Inc. in the spring of 1988 and included 43 classrooms in 10 schools in 6 large, mostly…

  10. "Diverse Providers" in Action: School Restructuring in Hawaii. Education Outlook. No. 8

    Science.gov (United States)

    Hess, Frederick M.; Squire, Juliet P.

    2009-01-01

    What to do about persistently low-performing schools is a pressing challenge for policymakers and educators across the nation. Schools that fail to make "adequate yearly progress" (AYP) for five consecutive years under No Child Left Behind (NCLB) must be "restructured." The 3,500 schools in the United States currently in…

  11. "Diverse Providers" in Action: School Restructuring in Hawaii. Working Paper 2009-03

    Science.gov (United States)

    Hess, Frederick M.; Squire, Juliet P.

    2009-01-01

    What to do about persistently low-performing schools is a pressing challenge for policymakers and educators across the nation. Schools that fail to make "adequate yearly progress" (AYP) for five consecutive years under No Child Left Behind (NCLB) must be "restructured". The 3,500 schools in the United States currently in…

  12. Avant le deluge: An investigation of some neglected dimensions of electricity restructuring in California

    Science.gov (United States)

    Golove, William Harry

    Chapter One of this dissertation focuses on describing the key participant groups and their principle interests in the restructuring process. The process of reaching agreement on the restructuring plan turned on each major interest group receiving satisfaction of all of its key objectives. Chapter Two explores the effective use of rhetoric in the electricity restructuring debate. Despite a lack of public involvement, the use of rhetoric appears to have been a prominent feature of the public policy process. A series of rhetorical concepts and mechanisms defined. Of these, the notions of rhetorical salience and rhetorical efficacy are most significant. The results of a field test on the impact of the particular examples of these tropes prominent in the restructuring debate on opinions regarding some of the key restructuring issues are presented. Chapter Three describes the sea change in the nature of demand side management (DSM) policy in the state, brought about by the restructuring of the electricity market. Whereas the primary goal of DSM had been resource acquisition, the new policy goal was to be market transformation. This chapter reviews the economic, and other social science theory that led to the development of demand side management programs based on a resource acquisition goal prior to restructuring and, subsequently, to a market transformation objective. Chapter Four examines the performance of the market during the period beginning in early 1998 through the first few months of 2000 at which time both the wholesale and retail markets in California were apparently functioning relatively well. The findings presented in this chapter are the result of a series of semi-structured interviews conducted with non-residential electricity customers that had chosen to receive electric service and, in some cases, value-added services from a competitive retail electricity service provider One of the key conclusions is that market rules appear to be critical to customer

  13. Study of the geopolymer restructuration by impulse rheology

    International Nuclear Information System (INIS)

    Rouyer, J.; Frizon, F.; Poulesquen, A.

    2015-01-01

    The aim of the study is to describe the evolution of the microstructure during the setting process of the geo-polymer using an original rheological method named Optimal Fourier Rheology (OFR). The alkali activation of meta-kaolin enables physicochemical transformation from a fresh paste to a hard meso-porous matrix. Classically, oscillatory rheology technique provides viscoelastic moduli spectrum and enables to determine rheological comportment of the material under investigation. However the duration to perform a complete spectrum (more than 2.5 h) makes useless this technique in the case of changing material. The OFR technique decreases the measurement duration under 10 minutes and enables to perform several snapshots of the evolving rheological behaviour. Contrary to monochromatic iterations, here the applied stress takes the form of a chirp function which contains the full usable bandwidth. Interpretations of spectrums provide efficient access to structural evolution along the setting. Results show that the number of oligomers increases into the solution due to the dissolution of the meta-kaolin leading to a constant increase of the viscoelastic parameters until the gradual appearance of the percolating networks. The gelling time was rigorously assessed by using the Winter and Chambon criterion. A fractal percolating network is formed inside the material after a reaction time depending on the formulation parameters; corresponding fractal dimensions were established. After the gel point, the viscoelastic moduli grow rapidly until geo-polymers reach a classic viscoelastic state. Structural unit size were determined using moduli curves crossover and equalled to 2.1 nm in the case of Na geo-polymer; this value fits extremely well with value previously obtained by SAXS. Finally, the elasticity becomes constant in a large frequency range and the viscous parameter strongly decreases which means that the solid porous network is under formation. In conclusion, this

  14. The effect of organisational restructuring on job satisfaction, career aspirations and stress levels of employees

    Directory of Open Access Journals (Sweden)

    E. N. Marais

    1996-06-01

    Full Text Available The field study reported here examined the effects of organisational restructuring on employees' job satisfaction, career aspirations and stress levels. Immediately after restructuring took place in the organisation, the employees affected by this advent were tested to determine whether restructuring would impact on the three variables. Eight months after restructuring in the organisation took place their job satisfaction, career aspirations and stress levels were measured again. The results of the research indicated no changes in job satisfaction levels, career aspirations or stress levels. The results provided no support for the expectation that after a time lapse of eight months the effects or organisational restructuring would diminish and that workers would experience a greater degree of job satisfaction. In addition, it would appear that the time lapse did not improve perceived career prospects or that stress levels decreased. Opsomming Die navorsing wat hier gerapporteer word/ het die effek van organisatoriese herstrukturering op werknemers se werkstevredenheid, loopbaanaspirasies en spanningsvlakke ondersoek. Onmiddellik nadat herstrukturering in die organisasie plaasgcvind het, is die werknemers wat deur die herstrukturering geraak is, getoets om vas te stel of herstrukturering 'n impak op die drie veranderlikes sou hê. Agt maande later is hul werkstevredenheid, loopbaanaspirasies en spanningsvlakke weer gemeet. Daar is gevind dat die werknemers se reaksie op organisatoriese herstrukturering onveranderd gebly het. Die resultate het geen steun verleen aan die verwagting dat die tydsverloop van agt maande die effek van organisatoriese herstrukturering sal verminder nie en dat werknemers tot 'n groter mate werkstevredenheid sal ervaar nie. Dit blyk verder dat die tydsverloop ook nie verbeterde loopbaanvooruitsigte tot gevolg gehad het of dat die werknemers se spanningsvlakke verlaag het nie.

  15. Science teachers' beliefs about teaching and reform: Case studies from a restructured high school

    Science.gov (United States)

    King, Elizabeth A.

    A qualitative research study of the beliefs of three science teachers about teaching and educational reform was carried out at a restructured high school belonging to the Coalition of Essential Schools (CES), a nationally prominent restructuring movement. One problem of educational reform is to sustain change in the science classroom. A new wave of reform is shifting the focus away from curriculum changes and towards professionalism of teachers empowered to restructure schools. The beliefs of the teachers are key to decisions made in the classroom. The teachers and staff of Metro High School adopted the Ten Common Principles of CES as their guide to restructuring and sustaining change. Changes included increased authority for teachers in shared decision making, increased staff time for professional development, grouping students heterogeneously, grouping students and faculty in teams for extended time periods, and organizing instruction around small group and individual student study (student-centered). The theoretical framework centers on the constructivist theory of learning, particularly Vygotsky's socio-cultural model, and Bakhtin's dialogic function of language. Nespor's belief system model was used to describe the four characteristic features of beliefs: episodic memories, alternativity, existential presumption, and evaluative loading. My research questions were: What memories of teaching have influenced the teachers? What are the teachers' beliefs about the learning environment? What are the teachers' beliefs about their students? What are the teachers' beliefs about student activities? Interviews were the primary data source for the case studies of the three teachers, with additional data from lesson plans, photo-voice, and other artifacts. The teachers shared many common beliefs including that strong peer support is necessary for reform. The teachers' beliefs allied themselves to the majority of the common principles of CES, especially personalization and

  16. Better sales networks.

    Science.gov (United States)

    Ustüner, Tuba; Godes, David

    2006-01-01

    Anyone in sales will tell you that social networks are critical. The more contacts you have, the more leads you'll generate, and, ultimately, the more sales you'll make. But that's a vast oversimplification. Different configurations of networks produce different results, and the salesperson who develops a nuanced understanding of social networks will outshine competitors. The salesperson's job changes over the course of the selling process. Different abilities are required in each stage of the sale: identifying prospects, gaining buy-in from potential customers, creating solutions, and closing the deal. Success in the first stage, for instance, depends on the salesperson acquiring precise and timely information about opportunities from contacts in the marketplace. Closing the deal requires the salesperson to mobilize contacts from prior sales to act as references. Managers often view sales networks only in terms of direct contacts. But someone who knows lots of people doesn't necessarily have an effective network because networks often pay off most handsomely through indirect contacts. Moreover, the density of the connections in a network is important. Do a salesperson's contacts know all the same people, or are their associates widely dispersed? Sparse networks are better, for example, at generating unique information. Managers can use three levers--sales force structure, compensation, and skills development--to encourage salespeople to adopt a network-based view and make the best possible use of social webs. For example, the sales force can be restructured to decouple lead generation from other tasks because some people are very good at building diverse ties but not so good at maintaining other kinds of networks. Companies that take steps of this kind to help their sales teams build better networks will reap tremendous advantages.

  17. The Glycolytic Metabolite, Fructose-1,6-bisphosphate, Blocks Epileptiform Bursts by Attenuating Voltage-Activated Calcium Currents in Hippocampal Slices

    Directory of Open Access Journals (Sweden)

    Li-Rong Shao

    2018-06-01

    Full Text Available Manipulation of metabolic pathways (e.g., ketogenic diet (KD, glycolytic inhibition alters neural excitability and represents a novel strategy for treatment of drug-refractory seizures. We have previously shown that inhibition of glycolysis suppresses epileptiform activity in hippocampal slices. In the present study, we aimed to examine the role of a “branching” metabolic pathway stemming off glycolysis (i.e., the pentose-phosphate pathway, PPP in regulating seizure activity, by using a potent PPP stimulator and glycolytic intermediate, fructose-1,6-bisphosphate (F1,6BP. Employing electrophysiological approaches, we investigated the action of F1,6BP on epileptiform population bursts, intrinsic neuronal firing, glutamatergic and GABAergic synaptic transmission and voltage-activated calcium currents (ICa in the CA3 area of hippocampal slices. Bath application of F1,6BP (2.5–5 mM blocked epileptiform population bursts induced in Mg2+-free medium containing 4-aminopyridine, in ~2/3 of the slices. The blockade occurred relatively rapidly (~4 min, suggesting an extracellular mechanism. However, F1,6BP did not block spontaneous intrinsic firing of the CA3 neurons (when synaptic transmission was eliminated with DNQX, AP-5 and SR95531, nor did it significantly reduce AMPA or NMDA receptor-mediated excitatory postsynaptic currents (EPSCAMPA and EPSCNMDA. In contrast, F1,6BP caused moderate reduction (~50% in GABAA receptor-mediated current, suggesting it affects excitatory and inhibitory synapses differently. Finally and unexpectedly, F1,6BP consistently attenuated ICa by ~40% without altering channel activation or inactivation kinetics, which may explain its anticonvulsant action, at least in this in vitro seizure model. Consistent with these results, epileptiform population bursts in CA3 were readily blocked by the nonspecific Ca2+ channel blocker, CdCl2 (20 μM, suggesting that these bursts are calcium dependent. Altogether, these data

  18. Electricity industry restructuring revisited: the case of Korea

    International Nuclear Information System (INIS)

    Lee, Byoung-Hoon; Ahn, Hyeon-Hyo

    2006-01-01

    In 2004, the Korean Government suspended its electricity market reform based on the two-thirds majority recommendation of a six-member joint study team. This suspension effectively interrupts the basic plan adopted in 1998 by the previous administration to divest and privatize Korea Electric Power Corporation's (KEPCO's) generation segment in 2000-2002, implement transmission open access and wholesale competition by 2008, and introduce retail competition thereafter. This policy-decision followed the controversial debate on electricity market reform in Korea. Reform proponents claim that electricity can be treated as ordinary goods exchangeable in the competitive market, and any problems caused by the transition to the market system are manageable. By contrast, reform opponents argue that effective competition of the power industry is not yet feasible due to the idiosyncratic nature of electricity (e.g., low-price elasticity of demand and not being storable at low cost) as well as the country's isolated electricity network. In suspending the electricity reform, the current administration accepted the final conclusion of the joint study team in the Tripartite Commission on the ground that the alleged benefits of reform are theoretical and uncertain, while the real costs and risks are substantial. (author)

  19. Restructuring and generation of electrical energy in the Iberian Peninsula

    International Nuclear Information System (INIS)

    Dominguez, E. Fernandez; Bernat, J. Xiberta

    2007-01-01

    Portugal and Spain are on the threshold of the creation of an Iberian electricity market. In order to help its development, the power of the electric interconnection between the countries has been increased and market mechanisms designed to resolve congestion, should it arise. A system of joint supply for the Iberian Peninsula will lead to single price for the whole area except at times when the interconnection is saturated, in which case prices will be somewhat higher in the importing zone. In the medium term, the hope is that both systems will have very similar generating equipment and that their variable costs will equalize due to the substitution of the most obsolete equipment with combined cycle power stations, and to the increase of exchange capacity. The coming into effect of this market will bring about improvements in the security and efficiency of supply in both countries. There will also be some obstacles to overcome, such as, for example, the current regulatory frame deficiencies on power generation, the contacts which exist at present in Portugal between the producers and the National Electricity Network, the asymmetry of the distribution channels in each country, the differences in rates and the limited capacity for exchange. (author)

  20. Industrial Restructuring and Urban Change in the Pittsburgh Region: Developmental, Ecological, and Socioeconomic Trade-offs

    Directory of Open Access Journals (Sweden)

    William Haller

    2005-06-01

    Full Text Available This article traces the steel industry's restructuring during the 1980s and its consequences for older industrial regions tied historically to steel production. These regions contained large working-class communities that declined because of deindustrialization and restructuring. This article first examines the transition of the steel industry from its roots in extractive and primary manufacturing to a scrap-recycling industry that minimizes labor and raw material inputs. This transition parallels the structural changes in other industries addressed by political economic perspectives, such as the new international division of labor and globalization of production. The article then focuses on the socioeconomic and structural changes, using the Pittsburgh region as an example, including the employment and land-use consequences of deindustrialization and the relationship between losses in manufacturing employment and increases in persistent joblessness and poverty associated with growth of the urban underclass.

  1. Broken dreams. Unmet expectations of investors in the Philippine electricity restructuring and privatization

    International Nuclear Information System (INIS)

    Roxas, Fernando; Santiago, Andrea

    2010-01-01

    Over the last three decades, privatization and restructuring of electricity sectors has been a hot topic. The expectation was that prices would fall due to increased competition and that generators would become more innovative and efficient. However, the enthusiasm for deregulation wavered after events such as the debacle in California, the fall of Enron and the setbacks of Ontario. In the Philippines, comprehensive legislation was passed in 2001 but the implementation has been rather slow and controversial. This paper looked at the allegation of politicizing power rates and found a logical alternative to the perceived conspiracy angle. Based on market data, it is highly probable that depressed market rates was a result of coping mechanisms of new players trying to adjust to the restructured industry. (author)

  2. Psychometric Evaluation of the MMPI-2/MMPI-2-RF Restructured Clinical Scales in an Israeli Sample.

    Science.gov (United States)

    Shkalim, Eleanor

    2015-10-01

    The current study cross-culturally evaluated the psychometric properties of the Minnesota Multiphasic Personality Inventory-2 (MMPI-2)/MMPI-2-Restructured Form Restructured Clinical (RC) Scales in psychiatric settings in Israel with a sample of 100 men and 133 women. Participants were administered the MMPI-2 and were rated by their therapists on a 188-item Patient Description Form. Results indicated that in most instances the RC Scales demonstrated equivalent or better internal consistencies and improved intercorrelation patterns relative to their clinical counterparts. Furthermore, external analyses revealed comparable or improved convergent validity (with the exceptions of Antisocial Behavior [RC4] and Ideas of Persecution [RC6] among men), and mostly greater discriminant validity. Overall, the findings indicate that consistent with previous findings, the RC Scales generally exhibit comparable to improved psychometric properties over the Clinical Scales. Implications of the results, limitations, and recommendations for future research are discussed. © The Author(s) 2014.

  3. Exploring science teachers' perceptions of experimentation: implications for restructuring school practical work

    Science.gov (United States)

    Wei, Bing; Li, Xiaoxiao

    2017-09-01

    It is commonly recognised that practical work has a distinctive and central role in science teaching and learning. Although a large number of studies have addressed the definitions, typologies, and purposes of practical work, few have consulted practicing science teachers. This study explored science teachers' perceptions of experimentation for the purpose of restructuring school practical work in view of science practice. Qualitative interviews were conducted with 87 science teachers at the secondary school level. In the interviews, science teachers were asked to make a comparison between students' experiments and scientific experiments. Eight dimensions of experimentation were generated from the qualitative data analysis, and the distributions of these eight dimensions between the two types of experiments were compared and analysed. An ideal model of practical work was suggested for restructuring practical work at the secondary school level, and some issues related to the effective enactment of practical work were discussed.

  4. Broken dreams: Unmet expectations of investors in the Philippine electricity restructuring and privatization

    Energy Technology Data Exchange (ETDEWEB)

    Roxas, Fernando, E-mail: fyanroxas@aim.ed [Asian Institute of Management, 123 Paseo de Roxas, Legaspi Village, Makati City (Philippines); Santiago, Andrea, E-mail: ma.andrea.santiago@dlsu.edu.p [De La Salle University, Taft Avenue, Manila (Philippines)

    2010-11-15

    Over the last three decades, privatization and restructuring of electricity sectors has been a hot topic. The expectation was that prices would fall due to increased competition and that generators would become more innovative and efficient. However, the enthusiasm for deregulation wavered after events such as the debacle in California, the fall of Enron and the setbacks of Ontario. In the Philippines, comprehensive legislation was passed in 2001 but the implementation has been rather slow and controversial. This paper looked at the allegation of politicizing power rates and found a logical alternative to the perceived conspiracy angle. Based on market data, it is highly probable that depressed market rates was a result of coping mechanisms of new players trying to adjust to the restructured industry.

  5. Broken dreams. Unmet expectations of investors in the Philippine electricity restructuring and privatization

    Energy Technology Data Exchange (ETDEWEB)

    Roxas, Fernando [Asian Institute of Management, 123 Paseo de Roxas, Legaspi Village, Makati City (Philippines); Santiago, Andrea [De La Salle University, Taft Avenue, Manila (Philippines)

    2010-11-15

    Over the last three decades, privatization and restructuring of electricity sectors has been a hot topic. The expectation was that prices would fall due to increased competition and that generators would become more innovative and efficient. However, the enthusiasm for deregulation wavered after events such as the debacle in California, the fall of Enron and the setbacks of Ontario. In the Philippines, comprehensive legislation was passed in 2001 but the implementation has been rather slow and controversial. This paper looked at the allegation of politicizing power rates and found a logical alternative to the perceived conspiracy angle. Based on market data, it is highly probable that depressed market rates was a result of coping mechanisms of new players trying to adjust to the restructured industry. (author)

  6. A survey of critical research areas in the energy segment of restructured electric power markets

    International Nuclear Information System (INIS)

    Nanduri, Vishnu; Das, Tapas K.

    2009-01-01

    Availability of a large volume of recent literature on deregulated (a.k.a. restructured) electricity markets underscores the importance of the research needs to ensure proper design and functioning of the markets. Researchers have made significant contributions fueling the evolution of the fundamental market design changes that have taken place since the beginning of the restructuring process. Due to the vast scope, existing survey papers are focused on particular facets of deregulated electricity markets. We adopt a similar approach by focusing on the most important research areas related to the energy market. The contributions of the survey paper lie in the novel approach used in classifying the literature based on critical research areas. Some areas of research such as auction based pricing, bidding strategy formulation, market equilibria, and market power are reviewed in a different light than other existing survey papers. We conclude by providing some future research directions for the energy markets. (author)

  7. Antioxidant Potential and Modulatory Effects of Restructured Lipids from the Amazonian Palms on Liver Cells

    Directory of Open Access Journals (Sweden)

    Andrea de Oliveira Falcão

    2017-01-01

    Full Text Available Enzymatic interesterification is used to manipulate oil and fat in order to obtain improved restructured lipids with desired technological properties. However, with raw materials containing significant amounts of bioactive compounds, the influence of this enzymatic process on the bioactivity of the final product is still not clear. Thus, the aim of this study is to evaluate the antioxidant potential and modulatory effects of two raw materials from the Amazonian area, buriti oil and murumuru fat, before and after lipase interesterification, on human hepatoma cells (HepG2. The results indicate that minor bioactive compounds naturally found in the raw materials and their antioxidant capacity are preserved after enzymatic interesterification, and that the restructured lipids modulate HepG2 endogenous antioxidant enzyme.

  8. Proceedings of the CERI 2003 electricity conference : electricity restructuring in transition. CD-ROM ed.

    International Nuclear Information System (INIS)

    2003-01-01

    The restructuring of the electric power industry was reviewed from a North American perspective. This conference focused on how current market structures can be improved to benefit all participants. The current issues for future development of power generation and transmission were discussed. The conference was divided into seven sessions as follows: (1) market design issues, (2) Federal Energy Regulatory Commission's (FERC's) wholesale market platform, (3) does retail competition make sense, (4) the future direction of the Alberta market, (5) the status of restructured electricity markets, (6) resource mix in a greenhouse gas (GHG)-constrained world, and (7) electricity supply from oil sands cogeneration. A total of eleven papers were selected and indexed separately for inclusion in this database. refs., tabs., figs

  9. Post-Soviet gas sector restructuring in the CIS: a political economy approach

    International Nuclear Information System (INIS)

    Hirschhausen, C. von; Engerer, H.

    1998-01-01

    This paper analyses progress and obstacles to gas sector reform in the most important CIS-Countries (Russia, Turkmenistan, Ukraine, Uzbekistan), taking a political economy perspective. This reform process is embedded in a very specific post-Soviet institutional framework stemming from the legacy of socialism. Firstly, we review the evolution of the gas sector for the period 1992-1998. The paper then identifies the post-Soviet specifies of gas sector restructuring, to which any reform strategy and technical assistance have to he adapted. We derive concrete, process-oriented policy conclusions to accelerate the reform process in a market-oriented way. The paper concludes with an evaluation of the perspectives of gas sector restructuring in this geopolitically strategic area of the world. (author)

  10. Effect of Oyster Shell Calcium Powder on the Quality of Restructured Pork Ham

    OpenAIRE

    Choi, Jung-Seok; Lee, Hyun-Jin; Jin, Sang-Keun; Lee, Hyun-Joo; Choi, Yang-Il

    2014-01-01

    This study was conducted to evaluate the effects of oyster shell calcium powder (OSCP) as a substitute for phosphates in curing agent, on the quality of restructured pork ham. Restructured pork ham was processed under six treatment conditions: T1 (no additives), T2 (0.3% sodium tripolyphosphate), T3 (1.5% NaCl+0.5% whey protein), T4 (1.5% NaCl+0.5% whey protein+0.15% OSCP), T5 (1.5% NaCl+0.5% whey protein+0.3% OSCP), and T6 (1.5% NaCl+0.5% whey protein+0.5% OSCP). Addition of OSCP significant...

  11. Who’s who in Business Portfolio Restructuring Research: The intellectual structure

    Directory of Open Access Journals (Sweden)

    Angélica María Sánchez-Riofrío

    2017-03-01

    Full Text Available There are few studies applying extensive qualitative and quantitative methods for addressing the research into Business Portfolio Restructuring (BPR. The aim of this paper is to identify the main authors, journals and studies that have informed the intellectual platform underpinning BPR as well as identifying the topics that have had the greatest impact. In this study, bibliographic references cited between 1959 and 2012, are analyzed using different bibliometric techniques. Three main results are found: first, the intellectual basis for this field involves a multidisciplinary and multifaceted literature, although the disciplines of finance and management are the principal contributors to research into BPR. Second, authors, journals and papers are identified to track the main framework of portfolio restructuring. Finally, the main forerunners of this study area are corporate strategy, divestitures, diversification and corporate governance. This is a study that contributes not only to the field of strategic management but also to information management.

  12. The environmental and efficiency effects of restructuring on the electric power sector in the United States. An empirical analysis

    International Nuclear Information System (INIS)

    Sharabaroff, Alexander; Boyd, Roy; Chimeli, Ariaster

    2009-01-01

    Recent measures to restructure the electric power sector in different US states have raised the interest of policy makers, commentators, and the general public as to the actual impact of restructuring on both the economy and the environment. This paper focuses on two aspects of restructuring, namely its potential impact on the efficiency of electricity generation and air pollution. Our empirical results suggest that restructuring contributes to improved efficiency of electricity generation and better air quality through reduced electricity-induced sulfur dioxide (SO 2 ) and carbon dioxide (CO 2 ) emissions, although no effect was found for emissions of nitrous oxides (NO x ). These results, in turn could have important implications for policy in this area. (author)

  13. After more than a decade of restructuring; what have we learned, and where are we heading

    International Nuclear Information System (INIS)

    Lowrie, W.G.

    1996-01-01

    The paper relates to the current state and future development of the global petroleum industry. The topic of this paper looks to the future, but it requires a review of the recent past. Specifically, the author examines, after a decade of restructuring and renewing, the experience obtained and the industrial heading. In this regard, the author mainly refers to the experiences obtained at the Amoco Corporation. 3 figs

  14. Did the expiration of retail price caps affect prices in the restructured Texas electricity market?

    International Nuclear Information System (INIS)

    Kang, Linhong; Zarnikau, Jay

    2009-01-01

    On January 1, 2007, the Electric Reliability Council of Texas (ERCOT) market became the first restructured market in the US to completely remove caps on the prices which could be charged to residential energy consumers by the retailers associated with the traditional or incumbent utility service providers. Our analysis suggests that the expiration of the price-to-beat (PTB) price caps may have led to a reduction in the average prices charged by competitive retail electric providers (REPs). (author)

  15. Development of market strategies of metallurgical enterrprises after restructuring of steel industry

    Directory of Open Access Journals (Sweden)

    B. Gajdzik

    2014-01-01

    Full Text Available Before metallurgical enterprises started implementation of marketing activities they had to go through restructuring processes which included all areas of their market activities. Privatised metallurgical enterprises after economic transformation gradually implemented marketing to their business activities. The article presents notions connected with development of marketing strategies from the period of last 20 years. The range of analysis includes categories corresponding with instruments of mix marketing (4P − product, price, place, promotion.

  16. Outsourcing, occupational restructuring, and employee well-being: Is there a silver lining?

    OpenAIRE

    Petri, Böckerman; Mika, Maliranta

    2012-01-01

    This paper examines the relationship between outsourcing and various aspects of employee well-being by devoting special attention to the role of occupational restructuring as a conveying mechanism. Using linked employer-employee data, we find that offshoring involves job destruction, especially when the destination is a low-wage country. In such circumstances, staying employees' job satisfaction is reduced. However, the relationship between outsourcing and employee well-being is not entirely ...

  17. What is the impact of bankrupt and restructured loans on Japanese bank efficiency?

    OpenAIRE

    Mamatzakis, Emmanuel; Matousek, Roman; Vu, Anh Nguyet

    2015-01-01

    The Japanese banking system provides a distinctive platform for the examination of the long-lasting effect of problem loans on efficiency. We measure technical efficiency by modifying a translog enhanced hyperbolic distance function with two undesirable outputs, identified as problem loans and problem other earning assets. Our unique database allows us to distinguish between bankrupt and restructured loans to investigate the underlying causality between these loans and efficiency. From the fl...

  18. Surface restructuring behavior of various types of poly(dimethylsiloxane) in water detected by SFG.

    Science.gov (United States)

    Chen, Chunyan; Wang, Jie; Chen, Zhan

    2004-11-09

    Surface structures of several different poly(dimethylsiloxane) (PDMS) materials, tetraethoxysilane-cured hydroxy-terminated PDMS (TEOS-PDMS), platinum-cured vinyl-terminated PDMS (Pt-PDMS), platinum-cured vinyl-terminated poly(diphenylsiloxane)-co-poly(dimethylsiloxane) (PDPS-co-PDMS), and PDMS-co-polystyrene (PDMS-co-PS) copolymer in air and water have been investigated by sum frequency generation (SFG) vibrational spectroscopy. The SFG spectra collected from all PDMS surfaces in both air and water are dominated by methyl group stretches, indicating that all the surfaces are mainly covered by methyl groups. Other than surface-dominating methyl groups, some -Si-CH2-CH2- moieties on the Pt-PDMS surface have also been detected in air, which are present at cross-linking points. Information about the average orientation angle and angle distribution of the methyl groups on the PDMS surface has been evaluated. Surface restructuring of the methyl groups has been observed for all PDMS surfaces in water. Upon contacting water, the methyl groups on all PDMS surfaces tilt more toward the surface. The detailed restructuring behaviors of several PDMS surfaces in water and the effects of molecular weight on restructuring behaviors have been investigated. For comparison, in addition to air and water, surface structures of PDMS materials mentioned above in a nonpolar solvent, FC-75, have also been studied. By comparing the different response of phenyl groups to water on both PDPS-co-PDMS and PS-co-PDMS surfaces, we have demonstrated how the restructuring behaviors of surface phenyl groups are affected by the structural flexibility of the molecular chains where they are attached.

  19. Restructuring of oil and gas companies in financial difficulty: the Canadian experience

    International Nuclear Information System (INIS)

    Hudec, A.J.

    1992-01-01

    The relationship, under Canadian law, between oil or gas company in financial difficulty and its various creditors is discussed. In particular, commercial bank lenders and non-defaulting co-venturers in the project are considered. The broad topics covered are: the financing of multi-party resource projects; recent developments in conventional oil and gas production loan facilities; alternative and new approaches to energy loan restructuring; minimizing the lender's environmental risk in realizing against an energy project. (UK)

  20. Recovery vs. Restructuring: Establishing Ecologic Patterns in Early and Middle Triassic Paleocommunities (Invited)

    Science.gov (United States)

    Fraiser, M.; Dineen, A.; Sheehan, P.

    2013-12-01

    Published data has been interpreted as indicating that marine ecological devastation following the end-Permian mass extinction was protracted and may have lasted 5 million years into the Middle Triassic (Anisian). However, a review of previous literature shows that understanding of biotic recovery is typically based on only a few components of the ecosystem, such as on taxonomic diversity, a single genus/phylum, or facies. Typically, paleocommunities are considered fully recovered when dominance and diversity are regained and normal ecosystem functioning has resumed. However, in addition to the biodiversity crash at the end of the Permian, taxonomic and ecologic structure also changed,with the extinction marking the faunal shift from brachiopod-rich Paleozoic Evolutionary Fauna (EF) to the mollusc-rich Modern EF. This suggests that the extreme reorganizational nature of the Triassic does not adhere to the standard definition of recovery, which is a return to previous conditions. Thus, we propose the term 'restructuring' to describe this interval, as Early and Middle Triassic communities might not exhibit the typical characteristics of a 'normal' Permian one. To more fully characterize Triassic ecologic restructuring, paleoecologists should take into account functional diversity and redundancy. We quantified functional richness and regularity in four different paleocommunities from classic Permian and Triassic sections. Functional richness was low in paleocommunities after the end-Permian mass extinction, but increased to high levels by the Middle Triassic. In contrast, functional regularity was low in the Middle Permian, but high in all the Triassic paleocommunities. The change from low to high functional regularity/redundancy at the P/T boundary may be a factor of the highly stressful Triassic environmental conditions (i.e. anoxia, hypercapnia), as high regularity in a community can boost survival in harsh environments. Parameters such as these will more

  1. UTILITY ADVANCED TURBINE SYSTEMS (ATS) TECHNOLOGY READINESS TESTING PHASE 3 RESTRUCTURED (3R)

    Energy Technology Data Exchange (ETDEWEB)

    Unknown

    2000-03-17

    This scope document defines the work scope for accomplishing the design of the GE MS7001H and MS9001H (7H and 9H) combined-cycle power systems under the original ATS Phase 3 DOE Cooperative Agreement No. DE-FC21-95MC31176, and incorporates changes in scope required to convert Phase 3 to the ''restructured'' Phase 3R as defined in Amendment A012 to the Cooperative Agreement.

  2. Impact de la restructuration de la taxe d'accise sur le tabac aux ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Aux Philippines, le taux de tabagisme est élevé et les taxes sur les produits du tabac, peu élevées. La meilleure façon de redresser la situation consiste à adopter des lois qui aug- mentent considérablement les taxes. Une loi restructurant les taxes sur les produits du tabac, qui comporte une forte hausse de la taxe d'accise ...

  3. Fast Effects of Cognitive Restructuring Training on Neurocognitive Functions in Opioid Addicts

    OpenAIRE

    Ehsan Tavakolian; Abbas Abolghasemi

    2016-01-01

    Aim of the study This study intended to investigate the effect of cognitive restructuring training on prefrontal related neurocognitive functions in opioid addicts and its relationship with relapse prevention. Subject or material and methods Thirty opioid addicts who completed a 21-day detoxification program were randomly placed in experimental and control groups. Before and after the training, the subjects underwent urinalysis, Addiction-Stroop test, Iowa Gambling Task, Wisconsin C...

  4. THE INDONESIAN BANK CRISIS AND RESTRUCTURING: LESSONS AND IMPLICATIONS FOR OTHER DEVELOPING COUNTRIES

    OpenAIRE

    Mari PANGESTU

    2003-01-01

    Drawing on the Indonesian experience in 1997, this paper demonstrates the complexity of managing banking crises deals. It aims at deriving conclusions from this experience for other developing countries facing similar problems. The paper also refers to comparative experiences of other countries, in particular Malaysia and Thailand, examines the IMF programme and reveals its shortcomings. The key question is to what extent the ineffectiveness and slow progress of bank restructuring, corporate ...

  5. CD147 subunit of lactate/H+ symporters MCT1 and hypoxia-inducible MCT4 is critical for energetics and growth of glycolytic tumors.

    Science.gov (United States)

    Le Floch, Renaud; Chiche, Johanna; Marchiq, Ibtissam; Naiken, Tanesha; Naïken, Tanesha; Ilc, Karine; Ilk, Karine; Murray, Clare M; Critchlow, Susan E; Roux, Danièle; Simon, Marie-Pierre; Pouysségur, Jacques

    2011-10-04

    Malignant tumors exhibit increased dependence on glycolysis, resulting in abundant export of lactic acid, a hypothesized key step in tumorigenesis. Lactic acid is mainly transported by two H(+)/lactate symporters, MCT1/MCT4, that require the ancillary protein CD147/Basigin for their functionality. First, we showed that blocking MCT1/2 in Ras-transformed fibroblasts with AR-C155858 suppressed lactate export, glycolysis, and tumor growth, whereas ectopic expression of MCT4 in these cells conferred resistance to MCT1/2 inhibition and reestablished tumorigenicty. A mutant-derivative, deficient in respiration (res(-)) and exclusively relying on glycolysis for energy, displayed low tumorigenicity. These res(-) cells could develop resistance to MCT1/2 inhibition and became highly tumorigenic by reactivating their endogenous mct4 gene, highlighting that MCT4, the hypoxia-inducible and tumor-associated lactate/H(+) symporter, drives tumorigenicity. Second, in the human colon adenocarcinoma cell line (LS174T), we showed that combined silencing of MCT1/MCT4 via inducible shRNA, or silencing of CD147/Basigin alone, significantly reduced glycolytic flux and tumor growth. However, both silencing approaches, which reduced tumor growth, displayed a low level of CD147/Basigin, a multifunctional protumoral protein. To gain insight into CD147/Basigin function, we designed experiments, via zinc finger nuclease-mediated mct4 and basigin knockouts, to uncouple MCTs from Basigin expression. Inhibition of MCT1 in MCT4-null, Basigin(high) cells suppressed tumor growth. Conversely, in Basigin-null cells, in which MCT activity had been maintained, tumorigenicity was not affected. Collectively, these findings highlight that the major protumoral action of CD147/Basigin is to control the energetics of glycolytic tumors via MCT1/MCT4 activity and that blocking lactic acid export provides an efficient anticancer strategy.

  6. Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity.

    Science.gov (United States)

    Hopperton, Kathryn E; Duncan, Robin E; Bazinet, Richard P; Archer, Michael C

    2014-01-15

    Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from (14)C-labeled acetate to those supplied exogenously as (14)C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2-3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells. © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

  7. Empowerment of Government Public Relations Office Post Restructuring ( Case Study on Informatics and Public Relations Office of Jakarta City Administration)

    OpenAIRE

    Patrianti, Tria

    2011-01-01

    This research aims to seek better understanding on the empowerment of the public relations office following the restructuring conducted by Jakarta City Administration. Involving 11 respondents, the research utilizes qualitative research method with case study approach.The background issues in the research are as followed: (1) Why the role and function of public relations office at Jakarta Administration remain insignificant regardless of the restructuring; (2) What the public relations offi...

  8. Calcium Alginate and Salt/Phosphate as Binding Agents in Restructured Lamb

    Directory of Open Access Journals (Sweden)

    Triana Setyawardani

    2001-01-01

    Full Text Available A study on restructurization of lamb meat using several binding agents were conducted. Objectives of the study were evaluate effectivity of Ca–alginate, salt and phosphate as binding agent and their effect on physical properties of the restructured meat stored at -20⁰C for up to 12 weeks. Three binding agents were added to the restructured products, which include NaCl 0.3 %/ NTPP 0.3 %; alginate 0.5 %/Ca-lactate 0.5%; NaCl 0.3 % / NTPP 0.5 %/alginate 0.5% and no binding agent as a control. The products were evaluated at 0, 4, 8 and 12 weeks of storage. The result showed that treatment with alginate 0.5%/Ca-lactate 0.5% had the least purge loss value of 4.3±0.2%. The least cooking losses of 30.2±3.79% and the highest shear force 61.6±13.77 N. (Animal Production 3(1: 20-25 (2001Key Words: Alginate/Ca-lactate, purge loss, cooking losses, shear force.

  9. Calcium Alginate and Salt/Phosphate as Binding Agents in Restructured Lamb

    Directory of Open Access Journals (Sweden)

    Triana Setyawardani

    2001-01-01

    Full Text Available A study on  restructurization of lamb meat using several binding agents were conducted. Objectives of the study were evaluate  effectivity of Ca–alginate, salt and phosphate as binding agent and their effect on physical properties of the restructured meat stored at -20⁰C for up to 12 weeks. Three binding agents were added to the restructured products, which include NaCl 0.3 %/ NTPP 0.3 %; alginate 0.5 %/Ca-lactate 0.5%; NaCl 0.3 % / NTPP 0.5 %/alginate 0.5% and no binding agent as a control. The products were evaluated at 0, 4, 8 and 12 weeks of storage. The result showed that treatment with alginate 0.5%/Ca-lactate 0.5% had the least purge loss value of 4.3±0.2%. The least cooking losses of 30.2±3.79% and the highest shear force 61.6±13.77 N. (Animal Production 3(1: 20-25 (2001 Key Words: Alginate/Ca-lactate, purge loss, cooking losses, shear  force.

  10. Financing investments in renewable energy: The role of policy design and restructuring

    Energy Technology Data Exchange (ETDEWEB)

    Wiser, R.; Pickle, S. [Lawrence Berkeley National Lab., CA (United States). Environmental Energy Technologies Div.

    1997-03-01

    The costs of electric power projects utilizing renewable energy technologies are highly sensitive to financing terms. Consequently, as the electricity industry is restructured and new renewables policies are created, it is important for policymakers to consider the impacts of renewables policy design on project financing. This report describes the power plant financing process and provides insights to policymakers on the important nexus between renewables policy design and finance. A cash-flow model is used to estimate the impact of various financing variables on renewable energy costs. Past and current renewable energy policies are then evaluated to demonstrate the influence of policy design on the financing process and on financing costs. The possible impacts of electricity restructuring on power plant financing are discussed and key design issues are identified for three specific renewable energy programs being considered in the restructuring process: (1) surcharge-funded policies; (2) renewables portfolio standards; and (3) green marketing programs. Finally, several policies that are intended to directly reduce financing costs and barriers are analyzed. The authors find that one of the key reasons that renewables policies are not more effective is that project development and financing processes are frequently ignored or misunderstood when designing and implementing renewable energy incentives. A policy that is carefully designed can reduce renewable energy costs dramatically by providing revenue certainty that will, in turn, reduce financing risk premiums.

  11. In their own words: how hospitals present corporate restructuring in their annual reports.

    Science.gov (United States)

    Arndt, M; Bigelow, B

    1999-01-01

    Hospitals operate in an environment with strong institutional pressures, in which legitimacy is critical to an organization's access to resources. In such an environment, organizations can increase their legitimacy by engaging in activities or discussing them in a manner that signals that the organization adheres to values held by its costituents. One important symbol of organizational actions or intentions is the formal organizational structure. When hospitals began to adopt a corporate structure in the early eighties, the way in which they presented this decision to the public was as important as the technical merits of the decision itself. This study investigates, through an analysis of annual reports, what hospitals signaled about their adoption of a corporate structure. The findings suggest that through restructuring, hospitals signaled that they were in line with practices advocated in the industry and literature (e.g., adhering to business values, protection of assets, or increasing patient services). By presenting multiple reasons for restructuring, hospitals could signal their attention to the needs of various constituents, and by touching only briefly on each reason, they could ignore the potential conflict between demands such as lower hospital cost and increased services. The findings also suggest that the first hospitals to adopt a corporate structure sought to educate constituents about restructuring by devoting a greater share of their annual report to the topic than later adopters and by enumerating a larger number of anticipated benefits from the structure, which would have enhanced the innovation's legitimacy in the early years.

  12. Restructuring of the jurisprudence course taught at the Canadian Memorial Chiropractic College

    Science.gov (United States)

    Gleberzon, Brian J.

    2010-01-01

    Introduction: The process by which the jurisprudence course was restructured at the Canadian Memorial Chiropractic College is chronicled. Method: A Delphi process used to restructure the course is described, and the results of a student satisfaction survey are presented. Results: When asked “I think this material was clinically relevant,” over 81% of the 76 students who respondents strongly agreed or agreed with this statement; 100% of students agreed or strongly agreed that scope of practice; marketing, advertising and internal office promotion; record keeping; fee schedules; malpractice issues and; professional malpractice issues and negligence was clinically relevant. When asked “I think this material was taught well,” a minimum of 89% of students agreed or strongly agreed with this statement. Discussion: This is the first article published that described the process by which a jurisprudence course was developed and assessed by student survey. Summary: Based on a survey of student perceptions, restructuring of the jurisprudence course was successful in providing students with clinically relevant information in an appropriate manner. This course may serve as an important first step in development a ‘model curriculum’ for chiropractic practice and the law courses in terms of content, format and assessment strategies. PMID:20195427

  13. Physicochemical Characteristics of Protein-Enriched Restructured Beef Steaks with Phosphates, Transglutaminase, and Elasticised Package Forming

    Directory of Open Access Journals (Sweden)

    Sephora Baugreet

    2018-01-01

    Full Text Available Restructured beef steaks were formulated by adding protein-rich ingredients (pea protein isolate (PPI, rice protein (RP, and lentil flour (LF (at 4 and 8%, phosphate (0.2%, and two binding agents: 1% (TG and 0.15% (TS. The effects of their addition on the physicochemical properties of the beef steaks were investigated. Protein content of the RP8TG sample was significantly higher than that of the control in both the raw and cooked state. Raw LF4TS exhibited greater (P<0.01 a∗ values than the control; however, after the cooking process, L∗, a∗, and b∗ values were similar for all treatments. Textural assessment showed that elevating protein level increased (P<0.001 hardness, chewiness, cohesiveness, and gumminess in cooked restructured steaks. LF addition reduced all textural values assessed, indicating a strong plant protein effect on texture modification. The commercial binder produced a better bind in combination with protein ingredients. This facilitated the production of uniformed restructured beef steaks from low-value beef muscles with acceptable quality parameters using a novel process technology.

  14. Science Lab Restructuring of a Public School Elementary and High School

    Directory of Open Access Journals (Sweden)

    Elisiane da Costa Moro

    2016-02-01

    Full Text Available This paper presents the process of restructuring the science lab of a state school in Caxias do Sul, whose main objective was to create a space where teachers could develop practical and experimental activities with their students. The restructuring of the science lab this school, was only possible through the project "More and Better Students and Teachers in Science, Mathematics, Engineering and Technologies" Initiation Program in Science and Mathematics, Engineering, Creative Technologies and Letters - PICMEL sponsored jointly by the University of Caxias do Sul, the SEDUC-RS, FAPERGS and CAPES. The project was developed at school by a teacher of physics and three high school students. Through the restructuring of the science lab, practical activities were developed and workshops where students had the opportunity to be more active in the process of teaching and learning. With the development of such activities was observed that the students were more willing to learn Science and Mathematics and could relate scientific knowledge to their daily lives, giving greater meaning to their learning.

  15. Nutritional composition in the chia seed and its processing properties on restructured ham-like products

    Directory of Open Access Journals (Sweden)

    Yi Ding

    2018-01-01

    Full Text Available Low-fat meat products always have harder texture, lower juiciness, and worse flavor. Due to their higher water-holding, water absorption, and organic molecule absorption, chia seeds (CHIA have been applied in powders, nutrition bars, breads, and cookies. Hence, the objectives of this study were to: (1 analyze the nutritional compositions in CHIA; and (2 look for the possible application of CHIA on restructured ham-like products. CHIA has high amounts of α-linolenic acid, crude polysaccharides, and also contains essential amino acids, minerals, and polyphenols. Regarding processing properties of CHIA, a combination of CHIA and carrageenan (CA increased (p<0.05 production yield of restructured ham-like products. A scanning electron microscope observation indicated that CHIA and CA addition can assist an emulsification in this ham-like product. Addition of 0.5% CA and 1.0% CHIA in this ham-like product showed the similar overall acceptance as products with added fat. Following storage at 4°C, higher (p<0.05 purge and centrifugation losses, as well as hardness of this ham-like product can be improved by adding CHIA and CA. CHIA addition also resulted in lower (p<0.05 lipid and protein oxidation, especially a 1.0% addition. In summary, due to both nutritional addition and improvements on physicochemical and sensorial properties of restructured ham-like products, CHIA seeds have great potential on the development of healthy and good-quality meat products.

  16. Current status and analysis of renewable promotional policies in Indian restructured power sector - A review

    International Nuclear Information System (INIS)

    Singh, Randhir; Sood, Yog Raj

    2011-01-01

    Restructuring has changed the traditional mission and mandates of power utilities in complex ways, and had large impacts on environmental, social, and political conditions for any particular country. At the same time, new regulatory approaches are being found for reducing environmental impacts in restructured power sectors. India has a vast supply of renewable energy resources, and it has one of the largest programs in the world for deploying renewable energy based products and systems. So this paper attempts to review the various policies and measures undertaken by Indian government for promotion of renewable energy. The aim of this paper is also to review the current policy mechanisms, especially investment- or generation-based price-driven and capacity-driven mechanisms, ranging from investment incentives for the development of renewable energy projects, feed-in tariffs, production tax incentives, tradable green certificates, and their effects upon the prospects of encouraging as well as expanding the development of renewable energy in Indian restructured power sector. This will make renewable more attractive in the Indian future electricity market. (author)

  17. Effect of comminution method and raw binder system in restructured beef.

    Science.gov (United States)

    Boles, J A; Shand, P J

    1998-07-01

    The effect of particle size, method of particle size reduction (grinding, flaking, slicing) and alginate or Fibrimex(™) binding systems on processing properties of restructured beef were investigated. The size of semimembranosus muscles was reduced with a meat slicer, grinder or flaker (Comitrol) through machine openings that were 2, 4 and 8 mm in size. Bind of meat pieces in raw and cooked beef steakettes were measured. Sensory evaluation to determine the effect of method of size reduction on texture of beef steakettes was done only on steakettes manufactured with alginate. Beef steakettes manufactured with the alginate binding system had significantly (p meat for the manufacture of restructured beef steakettes resulted in a higher raw bind than did either grinding or flaking the meat. Significant (p meat was sliced than when it was ground or flaked. However, when Fibrimex was used, bind was stronger when sliced meat was used. The alginate system resulted in higher binds for cooked product when particle size was smaller but with the Fibrimex system bind was higher when particle sizes were larger. A consumer panel on alginate restructured steakettes showed no preference for the texture produced by the different methods of particle size reduction.

  18. A restructuring of the MELCOR fission product packages for the MIDAS computer code

    International Nuclear Information System (INIS)

    Park, S.H.; Kim, K.R.; Kim, D.H.

    2004-01-01

    The RN1/RN2 packages, which are the fission product-related packages in MELCOR, have been restructured for the MIDAS computer code. MIDAS is being developed as an integrated severe accident analysis code with a user-friendly graphical user interface and a modernized data structure. To do this, the data transferring methods of the current MELCOR code are modified and adopted into the RN1/RN2 package. The data structure of the current MELCOR code using FORTRAN77 has a difficulty in grasping the meaning of the variables as well as waste of memory. New features of FORTRAN90 make it possible to allocate the storage dynamically and to user-defined data type, which leads to an efficient memory treatment and an easy understanding of the code. Restructuring of the RN1/RN2 package addressed in this paper includes a module development, subroutine modification, and the treatment of MELGEN, which generates the data file, as well as MELCOR, which is processing the calculation. The verification has been done by comparing the results of the modified code with those of the existing code. As the trends are similar to each other, it implies that the same approach could be extended to the entire code package. It is expected that the code restructuring will accelerate the code domestication thanks to a direct understanding of each variable and an easy implementation of the modified or newly developed models. (author)

  19. Essays on the investment behavior of independent power producers in the United States electricity industry under regulatory restructuring

    Science.gov (United States)

    Yan, Jingming

    2002-09-01

    In recent years, there have been efforts at both the federal and state level to introduce greater competition and markets into the US electricity industry through regulatory restructuring. A key to the success of such efforts is the ability of the restructuring to attract investment from non-utility, independent power producers (IPPs). The two essays in this dissertation examine empirically the investment behavior of IPPs under the regulatory restructuring between 1996 and 2000. In both essays, the effects of restructuring on a firm's investment decision are decomposed into the effects that work through the investment cost and that through the expected profit from the investment. The first essay studies the entry behavior of IPPs under the restructuring. The main finding of the essay is that the restructuring has done little to lower the entry barrier faced by IPPs-high fixed cost to entry is still a main factor that hinders IPP investment. The second essay studies IPPs' decisions between investing through building new power plants ("make") and investing through acquiring divested plants ("buy"). It finds that the availability of the "buy" option does not "squeeze" out investment on new capacities. IPPs that chose to "buy" did so because they expected a lower return from "make" and hence would not have switched their investment to new capacities even if the "buy" option were not available. Therefore, divestiture is a viable policy tool for state regulators to attract more IPP investment.

  20. Lifetime Optimization of a Multiple Sink Wireless Sensor Network through Energy Balancing

    Directory of Open Access Journals (Sweden)

    Tapan Kumar Jain

    2015-01-01

    Full Text Available The wireless sensor network consists of small limited energy sensors which are connected to one or more sinks. The maximum energy consumption takes place in communicating the data from the nodes to the sink. Multiple sink WSN has an edge over the single sink WSN where very less energy is utilized in sending the data to the sink, as the number of hops is reduced. If the energy consumed by a node is balanced between the other nodes, the lifetime of the network is considerably increased. The network lifetime optimization is achieved by restructuring the network by modifying the neighbor nodes of a sink. Only those nodes are connected to a sink which makes the total energy of the sink less than the threshold. This energy balancing through network restructuring optimizes the network lifetime. This paper depicts this fact through simulations done in MATLAB.