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Sample records for gene pool

  1. Sculpting the Barnyard Gene Pool

    Science.gov (United States)

    Childers, Gina; Wolfe, Kim; Dupree, Alan; Young, Sheila; Caver, Jessica; Quintanilla, Ruby; Thornton, Laura

    2016-01-01

    Project-based learning (PBL) takes student engagement to a higher level through reflective collaboration, inquiry, critical thinking, problem solving, and personal relevance. This article explains how six high school teachers developed an interconnected, interdisciplinary STEM-focused PBL called "Sculpting the Barnyard Gene Pool." The…

  2. Gene pool conservation of teak in Myanmar

    International Nuclear Information System (INIS)

    Tin-Tun

    1995-01-01

    Myanmar with an area of 261, 228 Sq. miles is endowed with various types of forests which occupied nearly 50% of the country. Teak (Tectona grandis Linn. f.) is one of the most valuable timber species for its excellent wood quality and properties which are not observed with other timbers. Gene pool can be defined as a group of individual trees growing over a wide range of environmental conditions, and constituting different genetic complexes which can be transmitted to the offsprings. Topics such as: objectives of gene pool conservation, genetically improved seeds for large scale forest plantations, methodology of conservation, are discussed in the article. Myanmar teak dominates the world's teak market, and thus it is crucial to maintain the superiority in the conservation of gene complexes of teak. To some extent, the conservation of gene pools of teak and tree improvements are being undertaken by the Forest Research Institute of Myanmar. It is felt that the dissemination of the philosophy and concept of gene conservation to the personal involved in the forestry activities of the country are still inadequate

  3. Conjugative plasmids: Vessels of the communal gene pool

    DEFF Research Database (Denmark)

    Norman, Anders; Hansen, Lars H.; Sørensen, Søren Johannes

    2009-01-01

    to the hosts and, potentially, other resident prokaryotes within specific environmental niches. Insight into the evolution of plasmid modules therefore contributes to our knowledge of gene dissemination and evolution within prokaryotic communities. This communal pool provides the prokaryotes with an important...... mechanistic framework for obtaining adaptability and functional diversity that alleviates the need for large genomes of specialized ‘private genes'....

  4. Gene Pools and the Genetic Architecture of Domesticated Cowpea

    Directory of Open Access Journals (Sweden)

    Bao-Lam Huynh

    2013-11-01

    Full Text Available Cowpea [ (L. Walp.] is a major tropical legume crop grown in warm to hot areas throughout the world and especially important to the people of sub-Saharan Africa where the crop was domesticated. To date, relatively little is understood about its domestication origins and patterns of genetic variation. In this study, a worldwide collection of cowpea landraces and African ancestral wild cowpea was genotyped with more than 1200 single nucleotide polymorphism markers. Bayesian inference revealed the presence of two major gene pools in cultivated cowpea in Africa. Landraces from gene pool 1 are mostly distributed in western Africa while the majority of gene pool 2 are located in eastern Africa. Each gene pool is most closely related to wild cowpea in the same geographic region, indicating divergent domestication processes leading to the formation of two gene pools. The total genetic variation within landraces from countries outside Africa was slightly greater than within African landraces. Accessions from Asia and Europe were more related to those from western Africa while accessions from the Americas appeared more closely related to those from eastern Africa. This delineation of cowpea germplasm into groups of genetic relatedness will be valuable for guiding introgression efforts in breeding programs and for improving the efficiency of germplasm management.

  5. The care and handling of the forest gene pool

    Science.gov (United States)

    Roy R. Silen; Ivan Doig

    1976-01-01

    What must be the world's most magnificent pool of forest genes has timbered our Pacific slopes.Why else do the tallest firs, pines, spruces, hemlocks, redwoods, and larches all rise along the Pacific Coast of North America? Does their hugeness simply thrust up from our deep soils and mild, rainy climate? From a vantage point of three...

  6. Gene pool conservation and tree improvement in Serbia

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    Isajev Vasilije

    2009-01-01

    Full Text Available This paper presents the concepts applied in the gene pool conservation and tree improvement in Serbia. Gene pool conservation of tree species in Serbia includes a series of activities aiming at the sustainability and protection of genetic and species variability. This implies the investigation of genetic resources and their identification through the research of the genetic structure and the breeding system of individual species. Paper also includes the study of intra- and inter-population variability in experiments - provenance tests, progeny tests, half- and full-sib lines, etc. The increased use of the genetic potential in tree improvement in Serbia should be intensified by the following activities: improvement of production of normal forest seed, application of the concept of new selections directed primarily to the improvement of only one character, because in that case the result would be certain, establishment and management of seed orchards as specialized plantations for long-term production of genetically good-quality forest seeds, and the shortening of the improvement process by introducing new techniques and methods (molecular markers, somaclonal variation, genetic engineering, protoplast fusion, micropropagation, etc..

  7. Nanobiotechnologies in the System of Farm Animals' Gene Pool Preservation

    Science.gov (United States)

    Kovtun, S. I.; Galagan, N. P.; Shcherbak, O. V.; Klymenko, N. Y.; Osypchuk, O. S.

    It has been determined that sperm viability and mobility upon long storage in liquid nitrogen is being reduced. This issue is relevant for gene pool objects' preservation and for reproductive medicine. The use of nanomaterials (NM) in reproductive biotechnology can expand to the methodology of the rational gene pool preservation technology, especially of valuable biomaterial. Use of nanoparticles, based on ultrafine silica (UFS) with different agents located on its surface gives a positive effect on the thawed sperm mobility, increasing its activity and survival rate and leading to increased fertilization ability. After thawing, the sperm of bulls had an average activity level of 47 %. This activity index of gametes in control was being maintained for 30 min. In the experimental groups after 30 min, the most active gametes were those with UFS + sucrose (52 %). A lower activity compared with the control and UFS + sucrose had gametes with UFS + D-galactosamine. Sperm activity with UFS + D-galactosamine decreased by 14 % compared with the control and by 19 % compared to the UFS + sucrose. So, the feasibility of adding NM with UFS + sucrose at a concentration of 0.001 % to thawed bull sperm that has been cryopreserved for a long time was proved.

  8. Combinatorial Pooling Enables Selective Sequencing of the Barley Gene Space

    Science.gov (United States)

    Lonardi, Stefano; Duma, Denisa; Alpert, Matthew; Cordero, Francesca; Beccuti, Marco; Bhat, Prasanna R.; Wu, Yonghui; Ciardo, Gianfranco; Alsaihati, Burair; Ma, Yaqin; Wanamaker, Steve; Resnik, Josh; Bozdag, Serdar; Luo, Ming-Cheng; Close, Timothy J.

    2013-01-01

    For the vast majority of species – including many economically or ecologically important organisms, progress in biological research is hampered due to the lack of a reference genome sequence. Despite recent advances in sequencing technologies, several factors still limit the availability of such a critical resource. At the same time, many research groups and international consortia have already produced BAC libraries and physical maps and now are in a position to proceed with the development of whole-genome sequences organized around a physical map anchored to a genetic map. We propose a BAC-by-BAC sequencing protocol that combines combinatorial pooling design and second-generation sequencing technology to efficiently approach denovo selective genome sequencing. We show that combinatorial pooling is a cost-effective and practical alternative to exhaustive DNA barcoding when preparing sequencing libraries for hundreds or thousands of DNA samples, such as in this case gene-bearing minimum-tiling-path BAC clones. The novelty of the protocol hinges on the computational ability to efficiently compare hundred millions of short reads and assign them to the correct BAC clones (deconvolution) so that the assembly can be carried out clone-by-clone. Experimental results on simulated data for the rice genome show that the deconvolution is very accurate, and the resulting BAC assemblies have high quality. Results on real data for a gene-rich subset of the barley genome confirm that the deconvolution is accurate and the BAC assemblies have good quality. While our method cannot provide the level of completeness that one would achieve with a comprehensive whole-genome sequencing project, we show that it is quite successful in reconstructing the gene sequences within BACs. In the case of plants such as barley, this level of sequence knowledge is sufficient to support critical end-point objectives such as map-based cloning and marker-assisted breeding. PMID:23592960

  9. Combinatorial pooling enables selective sequencing of the barley gene space.

    Directory of Open Access Journals (Sweden)

    Stefano Lonardi

    2013-04-01

    Full Text Available For the vast majority of species - including many economically or ecologically important organisms, progress in biological research is hampered due to the lack of a reference genome sequence. Despite recent advances in sequencing technologies, several factors still limit the availability of such a critical resource. At the same time, many research groups and international consortia have already produced BAC libraries and physical maps and now are in a position to proceed with the development of whole-genome sequences organized around a physical map anchored to a genetic map. We propose a BAC-by-BAC sequencing protocol that combines combinatorial pooling design and second-generation sequencing technology to efficiently approach denovo selective genome sequencing. We show that combinatorial pooling is a cost-effective and practical alternative to exhaustive DNA barcoding when preparing sequencing libraries for hundreds or thousands of DNA samples, such as in this case gene-bearing minimum-tiling-path BAC clones. The novelty of the protocol hinges on the computational ability to efficiently compare hundred millions of short reads and assign them to the correct BAC clones (deconvolution so that the assembly can be carried out clone-by-clone. Experimental results on simulated data for the rice genome show that the deconvolution is very accurate, and the resulting BAC assemblies have high quality. Results on real data for a gene-rich subset of the barley genome confirm that the deconvolution is accurate and the BAC assemblies have good quality. While our method cannot provide the level of completeness that one would achieve with a comprehensive whole-genome sequencing project, we show that it is quite successful in reconstructing the gene sequences within BACs. In the case of plants such as barley, this level of sequence knowledge is sufficient to support critical end-point objectives such as map-based cloning and marker-assisted breeding.

  10. Combinatorial pooling enables selective sequencing of the barley gene space.

    Science.gov (United States)

    Lonardi, Stefano; Duma, Denisa; Alpert, Matthew; Cordero, Francesca; Beccuti, Marco; Bhat, Prasanna R; Wu, Yonghui; Ciardo, Gianfranco; Alsaihati, Burair; Ma, Yaqin; Wanamaker, Steve; Resnik, Josh; Bozdag, Serdar; Luo, Ming-Cheng; Close, Timothy J

    2013-04-01

    For the vast majority of species - including many economically or ecologically important organisms, progress in biological research is hampered due to the lack of a reference genome sequence. Despite recent advances in sequencing technologies, several factors still limit the availability of such a critical resource. At the same time, many research groups and international consortia have already produced BAC libraries and physical maps and now are in a position to proceed with the development of whole-genome sequences organized around a physical map anchored to a genetic map. We propose a BAC-by-BAC sequencing protocol that combines combinatorial pooling design and second-generation sequencing technology to efficiently approach denovo selective genome sequencing. We show that combinatorial pooling is a cost-effective and practical alternative to exhaustive DNA barcoding when preparing sequencing libraries for hundreds or thousands of DNA samples, such as in this case gene-bearing minimum-tiling-path BAC clones. The novelty of the protocol hinges on the computational ability to efficiently compare hundred millions of short reads and assign them to the correct BAC clones (deconvolution) so that the assembly can be carried out clone-by-clone. Experimental results on simulated data for the rice genome show that the deconvolution is very accurate, and the resulting BAC assemblies have high quality. Results on real data for a gene-rich subset of the barley genome confirm that the deconvolution is accurate and the BAC assemblies have good quality. While our method cannot provide the level of completeness that one would achieve with a comprehensive whole-genome sequencing project, we show that it is quite successful in reconstructing the gene sequences within BACs. In the case of plants such as barley, this level of sequence knowledge is sufficient to support critical end-point objectives such as map-based cloning and marker-assisted breeding.

  11. Frozen gene pools - A future for species otherwise destined for extinction

    Science.gov (United States)

    Gee, G.F.

    1986-01-01

    Conclusion: Semen banks and ova and embryo banks can be practical methods to maintain gene pools. Gene pool preservation is desperately needed today due to the rapid decline in number of species and their habitat, a matter that is of concern to.biologists, economists, and politicians worldwide. Techniques are available for the cryopreservation of semen from many animals (and embryos from a few mammals) and adaptations of these techniques to other animals should be possible. A frozen gene pool in conjunction with existing programs makes it possible to preserve gene pools at less cost or in.some cases where no other alternative to extinction existed.

  12. Decrypting the mitochondrial gene pool of modern Panamanians.

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    Ugo A Perego

    Full Text Available The Isthmus of Panama--the narrow neck of land connecting the northern and southern American landmasses--was an obligatory corridor for the Paleo-Indians as they moved into South America. Archaeological evidence suggests an unbroken link between modern natives and their Paleo-Indian ancestors in some areas of Panama, even if the surviving indigenous groups account for only 12.3% of the total population. To evaluate if modern Panamanians have retained a larger fraction of the native pre-Columbian gene pool in their maternally-inherited mitochondrial genome, DNA samples and historical records were collected from more than 1500 volunteer participants living in the nine provinces and four indigenous territories of the Republic. Due to recent gene-flow, we detected ~14% African mitochondrial lineages, confirming the demographic impact of the Atlantic slave trade and subsequent African immigration into Panama from Caribbean islands, and a small European (~2% component, indicating only a minor influence of colonialism on the maternal side. The majority (~83% of Panamanian mtDNAs clustered into native pan-American lineages, mostly represented by haplogroup A2 (51%. These findings reveal an overwhelming native maternal legacy in today's Panama, which is in contrast with the overall concept of personal identity shared by many Panamanians. Moreover, the A2 sub-clades A2ad and A2af (with the previously named 6 bp Huetar deletion, when analyzed at the maximum level of resolution (26 entire mitochondrial genomes, confirm the major role of the Pacific coastal path in the peopling of North, Central and South America, and testify to the antiquity of native mitochondrial genomes in Panama.

  13. Deinococcus geothermalis: The Pool of Extreme Radiation Resistance Genes Shrinks

    Energy Technology Data Exchange (ETDEWEB)

    Makarova, Kira S.; Omelchenko, Marina V.; Gaidamakova, Elena K.; Matrosova, Vera Y.; Vasilenko, Alexander; Zhai, Min; Lapidus, Alla; Copeland, Alex; Kim, Edwin; Land, Miriam; Mavrommatis, Konstantinos; Pitluck, Samuel; Richardson, Paul M.; Detter, Chris; Brettin, Thomas; Saunders, Elizabeth; Lai, Barry; Ravel, Bruce; Kemner, Kenneth M.; Wolf, Yuri I.; Sorokin, Alexander; Gerasimova, Anna V.; Gelfand, Mikhail S.; Fredrickson, James K.; Koonin, Eugene V.; Daly, Michael J.

    2007-07-24

    Bacteria of the genus Deinococcus are extremely resistant to ionizing radiation (IR), ultraviolet light (UV) and desiccation. The mesophile Deinococcus radiodurans was the first member of this group whose genome was completely sequenced. Analysis of the genome sequence of D. radiodurans, however, failed to identify unique DNA repair systems. To further delineate the genes underlying the resistance phenotypes, we report the whole-genome sequence of a second Deinococcus species, the thermophile Deinococcus geothermalis, which at itsoptimal growth temperature is as resistant to IR, UV and desiccation as D. radiodurans, and a comparative analysis of the two Deinococcus genomes. Many D. radiodurans genes previously implicated in resistance, but for which no sensitive phenotype was observed upon disruption, are absent in D. geothermalis. In contrast, most D. radiodurans genes whose mutants displayed a radiation-sensitive phenotype in D. radiodurans are conserved in D. geothermalis. Supporting the existence of a Deinococcus radiation response regulon, a common palindromic DNA motif was identified in a conserved set of genes associated with resistance, and a dedicated transcriptional regulator was predicted. We present the case that these two species evolved essentially the same diverse set of gene families, and that the extreme stress-resistance phenotypes of the Deinococcus lineage emerged progressively by amassing cell-cleaning systems from different sources, but not by acquisition of novel DNA repair systems. Our reconstruction of the genomic evolution of the Deinococcus-Thermus phylum indicates that the corresponding set of enzymes proliferated mainly in the common ancestor of Deinococcus. Results of the comparative analysis weaken the arguments for a role of higher-order chromosome alignment structures in resistance; more clearly define and substantially revise downward the number of uncharacterized genes that might participate in DNA repair and contribute to

  14. Balancing gene expression without library construction via a reusable sRNA pool.

    Science.gov (United States)

    Ghodasara, Amar; Voigt, Christopher A

    2017-07-27

    Balancing protein expression is critical when optimizing genetic systems. Typically, this requires library construction to vary the genetic parts controlling each gene, which can be expensive and time-consuming. Here, we develop sRNAs corresponding to 15nt 'target' sequences that can be inserted upstream of a gene. The targeted gene can be repressed from 1.6- to 87-fold by controlling sRNA expression using promoters of different strength. A pool is built where six sRNAs are placed under the control of 16 promoters that span a ∼103-fold range of strengths, yielding ∼107 combinations. This pool can simultaneously optimize up to six genes in a system. This requires building only a single system-specific construct by placing a target sequence upstream of each gene and transforming it with the pre-built sRNA pool. The resulting library is screened and the top clone is sequenced to determine the promoter controlling each sRNA, from which the fold-repression of the genes can be inferred. The system is then rebuilt by rationally selecting parts that implement the optimal expression of each gene. We demonstrate the versatility of this approach by using the same pool to optimize a metabolic pathway (β-carotene) and genetic circuit (XNOR logic gate). © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  15. Dried blood spots of pooled samples for RHD gene screening in blood donors of mixed ancestry.

    Science.gov (United States)

    Silva-Malta, M C F; Araujo, N C Fidélis; Vieira, O V Neves; Schmidt, L Cayres; Gonçalves, P de Cassia; Martins, M Lobato

    2015-10-01

    In this study, we present a strategy for RHD gene screening based on real-time polymerase chain reaction (PCR) using dried blood spots of pooled samples. Molecular analysis of blood donors may be used to detect RHD variants among the presumed D-negative individuals. RHD genotyping using pooled samples is a strategy to test a large number of samples at a more reasonable cost. RHD gene detection based on real-time PCR using dried blood spots of pooled samples was standardised and used to evaluate 1550 Brazilian blood donors phenotyped as RhD-negative. Positive results were re-evaluated by retesting single samples using real-time PCR and conventional multiplex PCR to amplify five RHD-specific exons. PCR-sequence-specific primers was used to amplify RHDψ allele. We devised a strategy for RHD gene screening using dried blood spots of five pooled samples. Among 1550 serologically D-negative blood donors, 58 (3.74%) had the RHD gene. The non-functional RHDψ allele was detected in 47 samples (3.02%). The present method is a promising strategy to detect the RHD gene among presumed RhD-negative blood donors, particularly for populations with African ancestry. © 2015 British Blood Transfusion Society.

  16. Parts & Pools: A Framework for Modular Design of Synthetic Gene Circuits

    International Nuclear Information System (INIS)

    Marchisio, Mario Andrea

    2014-01-01

    Published in 2008, Parts & Pools represents one of the first attempts to conceptualize the modular design of bacterial synthetic gene circuits with Standard Biological Parts (DNA segments) and Pools of molecules referred to as common signal carriers (e.g., RNA polymerases and ribosomes). The original framework for modeling bacterial components and designing prokaryotic circuits evolved over the last years and brought, first, to the development of an algorithm for the automatic design of Boolean gene circuits. This is a remarkable achievement since gene digital circuits have a broad range of applications that goes from biosensors for health and environment care to computational devices. More recently, Parts & Pools was enabled to give a proper formal description of eukaryotic biological circuit components. This was possible by employing a rule-based modeling approach, a technique that permits a faithful calculation of all the species and reactions involved in complex systems such as eukaryotic cells and compartments. In this way, Parts & Pools is currently suitable for the visual and modular design of synthetic gene circuits in yeast and mammalian cells too.

  17. Parts & Pools: A Framework for Modular Design of Synthetic Gene Circuits

    Energy Technology Data Exchange (ETDEWEB)

    Marchisio, Mario Andrea, E-mail: marchisio@hit.edu.cn [School of Life Science and Technology, Harbin Institute of Technology, Harbin (China)

    2014-10-06

    Published in 2008, Parts & Pools represents one of the first attempts to conceptualize the modular design of bacterial synthetic gene circuits with Standard Biological Parts (DNA segments) and Pools of molecules referred to as common signal carriers (e.g., RNA polymerases and ribosomes). The original framework for modeling bacterial components and designing prokaryotic circuits evolved over the last years and brought, first, to the development of an algorithm for the automatic design of Boolean gene circuits. This is a remarkable achievement since gene digital circuits have a broad range of applications that goes from biosensors for health and environment care to computational devices. More recently, Parts & Pools was enabled to give a proper formal description of eukaryotic biological circuit components. This was possible by employing a rule-based modeling approach, a technique that permits a faithful calculation of all the species and reactions involved in complex systems such as eukaryotic cells and compartments. In this way, Parts & Pools is currently suitable for the visual and modular design of synthetic gene circuits in yeast and mammalian cells too.

  18. The Roles of Mitochondrion in Intergenomic Gene Transfer in Plants: A Source and a Pool

    Directory of Open Access Journals (Sweden)

    Nan Zhao

    2018-02-01

    Full Text Available Intergenomic gene transfer (IGT is continuous in the evolutionary history of plants. In this field, most studies concentrate on a few related species. Here, we look at IGT from a broader evolutionary perspective, using 24 plants. We discover many IGT events by assessing the data from nuclear, mitochondrial and chloroplast genomes. Thus, we summarize the two roles of the mitochondrion: a source and a pool. That is, the mitochondrion gives massive sequences and integrates nuclear transposons and chloroplast tRNA genes. Though the directions are opposite, lots of likenesses emerge. First, mitochondrial gene transfer is pervasive in all 24 plants. Second, gene transfer is a single event of certain shared ancestors during evolutionary divergence. Third, sequence features of homologies vary for different purposes in the donor and recipient genomes. Finally, small repeats (or micro-homologies contribute to gene transfer by mediating recombination in the recipient genome.

  19. Modular design of synthetic gene circuits with biological parts and pools.

    Science.gov (United States)

    Marchisio, Mario Andrea

    2015-01-01

    Synthetic gene circuits can be designed in an electronic fashion by displaying their basic components-Standard Biological Parts and Pools of molecules-on the computer screen and connecting them with hypothetical wires. This procedure, achieved by our add-on for the software ProMoT, was successfully applied to bacterial circuits. Recently, we have extended this design-methodology to eukaryotic cells. Here, highly complex components such as promoters and Pools of mRNA contain hundreds of species and reactions whose calculation demands a rule-based modeling approach. We showed how to build such complex modules via the joint employment of the software BioNetGen (rule-based modeling) and ProMoT (modularization). In this chapter, we illustrate how to utilize our computational tool for synthetic biology with the in silico implementation of a simple eukaryotic gene circuit that performs the logic AND operation.

  20. Gene expression changes in blood RNA after swimming in a chlorinated pool.

    Science.gov (United States)

    Salas, Lucas A; Font-Ribera, Laia; Bustamante, Mariona; Sumoy, Lauro; Grimalt, Joan O; Bonnin, Sarah; Aguilar, Maria; Mattlin, Heidi; Hummel, Manuela; Ferrer, Anna; Kogevinas, Manolis; Villanueva, Cristina M

    2017-08-01

    Exposure to disinfection by-products (DBP) such as trihalomethanes (THM) in swimming pools has been linked to adverse health effects in humans, but their biological mechanisms are unclear. We evaluated short-term changes in blood gene expression of adult recreational swimmers after swimming in a chlorinated pool. Volunteers swam 40min in an indoor chlorinated pool. Blood samples were drawn and four THM (chloroform, bromodichloromethane, dibromochloromethane and bromoform) were measured in exhaled breath before and after swimming. Intensity of physical activity was measured as metabolic equivalents (METs). Gene expression in whole blood mRNA was evaluated using IlluminaHumanHT-12v3 Expression-BeadChip. Linear mixed models were used to evaluate the relationship between gene expression changes and THM exposure. Thirty-seven before-after pairs were analyzed. The median increase from baseline to after swimming were: 0.7 to 2.3 for MET, and 1.4 to 7.1μg/m 3 for exhaled total THM (sum of the four THM). Exhaled THM increased on average 0.94μg/m 3 per 1 MET. While 1643 probes were differentially expressed post-exposure. Of them, 189 were also associated with exhaled levels of individual/total THM or MET after False Discovery Rate. The observed associations with the exhaled THM were low to moderate (Log-fold change range: -0.17 to 0.15). In conclusion, we identified short-term gene expression changes associated with swimming in a pool that were minor in magnitude and their biological meaning was unspecific. The high collinearity between exhaled THM levels and intensity of physical activity precluded mutually adjusted models with both covariates. These exploratory results should be validated in future studies. Copyright © 2017. Published by Elsevier B.V.

  1. Nucleotide Pool Depletion Induces G-Quadruplex-Dependent Perturbation of Gene Expression

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    Charikleia Papadopoulou

    2015-12-01

    Full Text Available Nucleotide pool imbalance has been proposed to drive genetic instability in cancer. Here, we show that slowing replication forks by depleting nucleotide pools with hydroxyurea (HU can also give rise to both transient and permanent epigenetic instability of a reporter locus, BU-1, in DT40 cells. HU induces stochastic formation of Bu-1low variants in dividing cells, which have lost the H3K4me3 present in untreated cells. This instability is potentiated by an intragenic G quadruplex, which also promotes local H2Ax phosphorylation and transient heterochromatinization. Genome-wide, gene expression changes induced by HU significantly overlap with those resulting from loss of the G4-helicases FANCJ, WRN, and BLM. Thus, the effects of global replication stress induced by nucleotide pool depletion can be focused by local replication impediments caused by G quadruplex formation to induce epigenetic instability and changes in gene expression, a mechanism that may contribute to selectable transcriptional changes in cancer.

  2. Two endornaviruses show differential infection patterns between gene pools of Phaseolus vulgaris.

    Science.gov (United States)

    Khankhum, Surasak; Valverde, Rodrigo A; Pastor-Corrales, Marcial A; Osorno, Juan M; Sabanadzovic, Sead

    2015-04-01

    We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2, in breeding lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) collected from the two centers of common bean domestication: Mesoamerica and the Andes. The two endornaviruses were detected in many genotypes of Mesoamerican origin but rarely in genotypes of Andean origin. The results suggest that these two endornaviruses were introduced into the Mesoamerican modern genotypes during common bean domestication and provide more evidence for the existence of two divergent gene pools of common bean.

  3. Genomics of crop wild relatives: expanding the gene pool for crop improvement.

    Science.gov (United States)

    Brozynska, Marta; Furtado, Agnelo; Henry, Robert J

    2016-04-01

    Plant breeders require access to new genetic diversity to satisfy the demands of a growing human population for more food that can be produced in a variable or changing climate and to deliver the high-quality food with nutritional and health benefits demanded by consumers. The close relatives of domesticated plants, crop wild relatives (CWRs), represent a practical gene pool for use by plant breeders. Genomics of CWR generates data that support the use of CWR to expand the genetic diversity of crop plants. Advances in DNA sequencing technology are enabling the efficient sequencing of CWR and their increased use in crop improvement. As the sequencing of genomes of major crop species is completed, attention has shifted to analysis of the wider gene pool of major crops including CWR. A combination of de novo sequencing and resequencing is required to efficiently explore useful genetic variation in CWR. Analysis of the nuclear genome, transcriptome and maternal (chloroplast and mitochondrial) genome of CWR is facilitating their use in crop improvement. Genome analysis results in discovery of useful alleles in CWR and identification of regions of the genome in which diversity has been lost in domestication bottlenecks. Targeting of high priority CWR for sequencing will maximize the contribution of genome sequencing of CWR. Coordination of global efforts to apply genomics has the potential to accelerate access to and conservation of the biodiversity essential to the sustainability of agriculture and food production. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  4. Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall. DC. (Fabaceae

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    A. B. Kholina

    2015-05-01

    Full Text Available In order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall. DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; also we studied the response of seeds to deep freezing in liquid nitrogen (–196 ºС. Population has an average level of polymorphism (P95 = 41,2 %, P99 = 52,9 %, A = 1,58, Ho = 0,158, He = 0,171 in general typical for herbaceous legumes, and can serve as a source of material for gene pool conservation of the species. Deep freezing has not led to the death of the seeds; it was marked stimulatory effect of ultralow temperatures, expressed as an acceleration of germination and sharp increase of germinability (98,6 ± 2,3 % compared to the control (12,0 ± 3,5 % that is associated with overcoming physical dormancy. There were no abnormalities in the development of seedlings from seeds passed cryopreservation.

  5. The Polynesian gene pool: an early contribution by Amerindians to Easter Island.

    Science.gov (United States)

    Thorsby, Erik

    2012-03-19

    It is now generally accepted that Polynesia was first settled by peoples from southeast Asia. An alternative that eastern parts of Polynesia were first inhabited by Amerindians has found little support. There are, however, many indications of a 'prehistoric' (i.e. before Polynesia was discovered by Europeans) contact between Polynesia and the Americas, but genetic evidence of a prehistoric Amerindian contribution to the Polynesian gene pool has been lacking. We recently carried out genomic HLA (human leucocyte antigen) typing as well as typing for mitochondrial DNA (mtDNA) and Y chromosome markers of blood samples collected in 1971 and 2008 from reputedly non-admixed Easter Islanders. All individuals carried HLA alleles and mtDNA types previously found in Polynesia, and most of the males carried Y chromosome markers of Polynesian origin (a few had European Y chromosome markers), further supporting an initial Polynesian population on Easter Island. The HLA investigations revealed, however, that some individuals also carried HLA alleles which have previously almost only been found in Amerindians. We could trace the introduction of these Amerindian alleles to before the Peruvian slave trades, i.e. before the 1860s, and provide suggestive evidence that they were introduced already in prehistoric time. Our results demonstrate an early Amerindian contribution to the Polynesian gene pool on Easter Island, and illustrate the usefulness of typing for immunogenetic markers such as HLA to complement mtDNA and Y chromosome analyses in anthropological investigations.

  6. Subgenomic Diversity Patterns Caused by Directional Selection in Bread Wheat Gene Pools

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    Kai Voss-Fels

    2015-07-01

    Full Text Available Genetic diversity represents the fundamental key to breeding success, providing the basis for breeders to select varieties with constantly improving yield performance. On the other hand, strong selection during domestication and breeding have eliminated considerable genetic diversity in the breeding pools of major crops, causing erosion of genetic potential for adaptation to emerging challenges like climate change. High-throughput genomic technologies can address this dilemma by providing detailed knowledge to characterize and replenish genetic diversity in breeding programs. In hexaploid bread wheat ( L., the staple food for 35% of the world’s population, bottlenecks during allopolyploidisation followed by strong artificial selection have considerably narrowed diversity to the extent that yields in many regions appear to be unexpectedly stagnating. In this study, we used a 90,000 single nucleotide polymorphism (SNP wheat genotyping array to assay high-frequency, polymorphic SNP markers in 460 accessions representing different phenological diversity groups from Asian, Australian, European, and North American bread wheat breeding materials. Detailed analysis of subgroup diversity at the chromosome and subgenome scale revealed highly distinct patterns of conserved linkage disequilibrium between different gene pools. The data enable identification of genome regions in most need of rejuvenation with novel diversity and provide a high-resolution molecular basis for genomic-assisted introgression of new variation into chromosome segments surrounding directionally selected metaloci conferring important adaptation and quality traits.

  7. Further evidence of an Amerindian contribution to the Polynesian gene pool on Easter Island.

    Science.gov (United States)

    Thorsby, E; Flåm, S T; Woldseth, B; Dupuy, B M; Sanchez-Mazas, A; Fernandez-Vina, M A

    2009-06-01

    Available evidence suggests a Polynesian origin of the Easter Island population. We recently found that some native Easter Islanders also carried some common American Indian (Amerindian) human leukocyte antigen (HLA) alleles, which probably were introduced before Europeans discovered the island in 1722. In this study, we report molecular genetic investigations of 21 other selected native Easter Islanders. Analysis of mitochondrial DNA and Y chromosome markers showed no traces of an Amerindian contribution. However, high-resolution genomic HLA typing showed that two individuals carried some other common Amerindian HLA alleles, different from those found in our previous investigations. The new data support our previous evidence of an Amerindian contribution to the gene pool on Easter Island.

  8. Wheat Landraces Are Better Qualified as Potential Gene Pools at Ultraspaced rather than Densely Grown Conditions

    Directory of Open Access Journals (Sweden)

    Elissavet G. Ninou

    2014-01-01

    Full Text Available The negative relationship between the yield potential of a genotype and its competitive ability may constitute an obstacle to recognize outstanding genotypes within heterogeneous populations. This issue was investigated by growing six heterogeneous wheat landraces along with a pure-line commercial cultivar under both dense and widely spaced conditions. The performance of two landraces showed a perfect match to the above relationship. Although they lagged behind the cultivar by 64 and 38% at the dense stand, the reverse was true with spaced plants where they succeeded in out-yielding the cultivar by 58 and 73%, respectively. It was concluded that dense stand might undervalue a landrace as potential gene pool in order to apply single-plant selection targeting pure-line cultivars, attributable to inability of plants representing high yielding genotypes to exhibit their capacity due to competitive disadvantage. On the other side, the yield expression of individuals is optimized when density is low enough to preclude interplant competition. Therefore, the latter condition appears ideal to identify the most promising landrace for breeding and subsequently recognize the individuals representing the most outstanding genotypes.

  9. Analysis of pools of targeted Salmonella deletion mutants identifies novel genes affecting fitness during competitive infection in mice.

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    Carlos A Santiviago

    2009-07-01

    Full Text Available Pools of mutants of minimal complexity but maximal coverage of genes of interest facilitate screening for genes under selection in a particular environment. We constructed individual deletion mutants in 1,023 Salmonella enterica serovar Typhimurium genes, including almost all genes found in Salmonella but not in related genera. All mutations were confirmed simultaneously using a novel amplification strategy to produce labeled RNA from a T7 RNA polymerase promoter, introduced during the construction of each mutant, followed by hybridization of this labeled RNA to a Typhimurium genome tiling array. To demonstrate the ability to identify fitness phenotypes using our pool of mutants, the pool was subjected to selection by intraperitoneal injection into BALB/c mice and subsequent recovery from spleens. Changes in the representation of each mutant were monitored using T7 transcripts hybridized to a novel inexpensive minimal microarray. Among the top 120 statistically significant spleen colonization phenotypes, more than 40 were mutations in genes with no previously known role in this model. Fifteen phenotypes were tested using individual mutants in competitive assays of intraperitoneal infection in mice and eleven were confirmed, including the first two examples of attenuation for sRNA mutants in Salmonella. We refer to the method as Array-based analysis of cistrons under selection (ABACUS.

  10. Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch.

    Science.gov (United States)

    Poulain, Adeline; Perret, Sylvie; Malenfant, Félix; Mullick, Alaka; Massie, Bernard; Durocher, Yves

    2017-08-10

    To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene expression (TGE). We have developed a cell line (CHO BRI/rcTA ) allowing the inducible expression of recombinant proteins, based on the cumate gene switch. After the identification of optimal plasmid DNA topology (supercoiled vs linearized plasmid) for PEIpro™ mediated transfection and of optimal conditions for methionine sulfoximine (MSX) selection, we were able to generate CHO BRI/rcTA pools producing high levels of recombinant proteins. Volumetric productivities of up to 900mg/L were reproducibly achieved for a Fc fusion protein and up to 350mg/L for an antibody after 14days post-induction in non-optimized fed-batch cultures. In addition, we show that CHO pool volumetric productivities are not affected by a freeze-thaw cycle or following maintenance in culture for over one month in the presence of MSX. Finally, we demonstrate that volumetric protein production with the CR5 cumate-inducible promoter is three- to four-fold higher than with the human CMV or hybrid EF1α-HTLV constitutive promoters. These results suggest that the cumate-inducible CHO BRI/rcTA stable pool platform is a powerful and robust system for the rapid production of gram amounts of recombinant proteins. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

  11. Adding to Yersinia enterocolitica Gene Pool Diversity: Two Cryptic Plasmids from a Biotype 1A Isolate

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    Daniela Lepka

    2009-01-01

    Full Text Available We report the nucleotide sequence of two novel cryptic plasmids (4357 and 14 662 base pairs carried by a Yersinia enterocolitica biotype 1A strain isolated from pork. As distinguished from most biotype 1A strains, this isolate, designated 07-04449, exhibited adherence to eukaryotic cells. The smaller plasmid pYe4449-1 carries five attributable open reading frames (ORFs encoding the first CcdA/CcdB-like antitoxin/toxin system described for a Yersinia plasmid, a RepA-like replication initiation protein, and mobilizing factors MobA and MobC. The deduced amino acid sequences showed highest similarity to proteins described in Salmonella (CcdA/B, Klebsiella (RepA, and Plesiomonas (MobA/C indicating genomic fluidity among members of the Enterobacteriaceae. One additional ORF with unknown function, termed ORF5, was identified with an ancestry distinct from the rest of the plasmid. While the C+G content of ORF5 is 38.3%, the rest of pYe4449-1 shows a C+G content of 55.7%. The C+G content of the larger plasmid pYe4449-2 (54.9% was similar to that of pYe4449-1 (53.7% and differed from that of the Y. enterocolitica genome (47.3%. Of the 14 ORFs identified on pYe4449-2, only six ORFs showed significant similarity to database entries. For three of these ORFs likely functions could be ascribed: a TnpR-like resolvase and a phage replication protein, localized each on a low C+G island, and DNA primase TraC. Two ORFs of pYe4449-2, ORF3 and ORF7, seem to encode secretable proteins. Epitope-tagging of ORF3 revealed protein expression at 4°C but not at or above 27°C suggesting adaptation to a habitat outside swine. The hypothetical protein encoded by ORF7 is the member of a novel repeat protein family sharing the DxxGN(xnDxxGN motif. Our findings illustrate the exceptional gene pool diversity within the species Y. enterocolitica driven by horizontal gene transfer events.

  12. Pooled Enrichment Sequencing Identifies Diversity and Evolutionary Pressures at NLR Resistance Genes within a Wild Tomato Population.

    Science.gov (United States)

    Stam, Remco; Scheikl, Daniela; Tellier, Aurélien

    2016-06-02

    Nod-like receptors (NLRs) are nucleotide-binding domain and leucine-rich repeats containing proteins that are important in plant resistance signaling. Many of the known pathogen resistance (R) genes in plants are NLRs and they can recognize pathogen molecules directly or indirectly. As such, divergence and copy number variants at these genes are found to be high between species. Within populations, positive and balancing selection are to be expected if plants coevolve with their pathogens. In order to understand the complexity of R-gene coevolution in wild nonmodel species, it is necessary to identify the full range of NLRs and infer their evolutionary history. Here we investigate and reveal polymorphism occurring at 220 NLR genes within one population of the partially selfing wild tomato species Solanum pennellii. We use a combination of enrichment sequencing and pooling ten individuals, to specifically sequence NLR genes in a resource and cost-effective manner. We focus on the effects which different mapping and single nucleotide polymorphism calling software and settings have on calling polymorphisms in customized pooled samples. Our results are accurately verified using Sanger sequencing of polymorphic gene fragments. Our results indicate that some NLRs, namely 13 out of 220, have maintained polymorphism within our S. pennellii population. These genes show a wide range of πN/πS ratios and differing site frequency spectra. We compare our observed rate of heterozygosity with expectations for this selfing and bottlenecked population. We conclude that our method enables us to pinpoint NLR genes which have experienced natural selection in their habitat. © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  13. Pooled Enrichment Sequencing Identifies Diversity and Evolutionary Pressures at NLR Resistance Genes within a Wild Tomato Population

    Science.gov (United States)

    Stam, Remco; Scheikl, Daniela; Tellier, Aurélien

    2016-01-01

    Nod-like receptors (NLRs) are nucleotide-binding domain and leucine-rich repeats containing proteins that are important in plant resistance signaling. Many of the known pathogen resistance (R) genes in plants are NLRs and they can recognize pathogen molecules directly or indirectly. As such, divergence and copy number variants at these genes are found to be high between species. Within populations, positive and balancing selection are to be expected if plants coevolve with their pathogens. In order to understand the complexity of R-gene coevolution in wild nonmodel species, it is necessary to identify the full range of NLRs and infer their evolutionary history. Here we investigate and reveal polymorphism occurring at 220 NLR genes within one population of the partially selfing wild tomato species Solanum pennellii. We use a combination of enrichment sequencing and pooling ten individuals, to specifically sequence NLR genes in a resource and cost-effective manner. We focus on the effects which different mapping and single nucleotide polymorphism calling software and settings have on calling polymorphisms in customized pooled samples. Our results are accurately verified using Sanger sequencing of polymorphic gene fragments. Our results indicate that some NLRs, namely 13 out of 220, have maintained polymorphism within our S. pennellii population. These genes show a wide range of πN/πS ratios and differing site frequency spectra. We compare our observed rate of heterozygosity with expectations for this selfing and bottlenecked population. We conclude that our method enables us to pinpoint NLR genes which have experienced natural selection in their habitat. PMID:27189991

  14. Population structure of the malaria vector Anopheles sinensis (Diptera: Culicidae in China: two gene pools inferred by microsatellites.

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    Yajun Ma

    Full Text Available BACKGROUND: Anopheles sinensis is a competent malaria vector in China. An understanding of vector population structure is important to the vector-based malaria control programs. However, there is no adequate data of A. sinensis population genetics available yet. METHODOLOGY/PRINCIPAL FINDINGS: This study used 5 microsatellite loci to estimate population genetic diversity, genetic differentiation and demographic history of A. sinensis from 14 representative localities in China. All 5 microsatellite loci were highly polymorphic across populations, with high allelic richness and heterozygosity. Hardy-Weinberg disequilibrium was found in 12 populations associated with heterozygote deficits, which was likely caused by the presence of null allele and the Wahlund effect. Bayesian clustering analysis revealed two gene pools, grouping samples into two population clusters; one includes six and the other includes eight populations. Out of 14 samples, six samples were mixed with individuals from both gene pools, indicating the coexistence of two genetic units in the areas sampled. The overall differentiation between two genetic pools was moderate (F(ST = 0.156. Pairwise differentiation between populations were lower within clusters (F(ST = 0.008-0.028 in cluster I and F(ST = 0.004-0.048 in cluster II than between clusters (F(ST = 0.120-0.201. A reduced gene flow (Nm = 1-1.7 was detected between clusters. No evidence of isolation by distance was detected among populations neither within nor between the two clusters. There are differences in effective population size (Ne = 14.3-infinite across sampled populations. CONCLUSIONS/SIGNIFICANCE: Two genetic pools with moderate genetic differentiation were identified in the A. sinensis populations in China. The population divergence was not correlated with geographic distance or barrier in the range. Variable effective population size and other demographic effects of historical population

  15. Expansion of the known Klebsiella pneumoniae species gene pool by characterization of novel alien DNA islands integrated into tmRNA gene sites.

    Science.gov (United States)

    Zhang, Jie; van Aartsen, Jon Jurriaan; Jiang, Xiaofei; Shao, Yucheng; Tai, Cui; He, Xinyi; Tan, Zhilei; Deng, Zixin; Jia, Shiru; Rajakumar, Kumar; Ou, Hong-Yu

    2011-02-01

    Klebsiella pneumoniae is an important bacterial pathogen of man that is commonly associated with opportunistic and hospital-associated infections. Increasing levels of multiple-antibiotic resistance associated with this species pose a major emerging clinical problem. This organism also occurs naturally in other diverse environments, including the soil. Consistent with its varied lifestyle and membership of the Enterobacteriaceae family, K. pneumoniae genomes exhibit highly plastic architecture comprising a core genome backbone interspersed with numerous and varied alien genomic islands. In this study the size of the presently known K. pneumoniae pan-genome gene pool was estimated through analysis of complete sequences of three chromosomes and 31 plasmids belonging to K. pneumoniae strains. In addition, using a PCR-based strategy the genomic content of eight tRNA/tmRNA gene sites that serve as DNA insertion hotspots were investigated in 28 diverse environmental and clinical strains of K. pneumoniae. Sequencing and characterization of five newly identified horizontally-acquired tmRNA-associated islands further expanded the archived K. pneumoniae gene pool to a total of 7648 unique gene members. Large-scale investigation of the content of tRNA/tmRNA hotspots will be useful to identify and/or survey accessory sequences dispersed amongst hundreds to thousands of members of many key bacterial species. Copyright © 2010 Elsevier B.V. All rights reserved.

  16. Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

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    Carla Ceoloni

    2017-12-01

    Full Text Available Wild species are extremely rich resources of useful genes not available in the cultivated gene pool. For species providing staple food to mankind, such as the cultivated Triticum species, including hexaploid bread wheat (Triticum aestivum, 6x and tetraploid durum wheat (T. durum, 4x, widening the genetic base is a priority and primary target to cope with the many challenges that the crop has to face. These include recent climate changes, as well as actual and projected demographic growth, contrasting with reduction of arable land and water reserves. All of these environmental and societal modifications pose major constraints to the required production increase in the wheat crop. A sustainable approach to address this task implies resorting to non-conventional breeding strategies, such as “chromosome engineering”. This is based on cytogenetic methodologies, which ultimately allow for the incorporation into wheat chromosomes of targeted, and ideally small, chromosomal segments from the genome of wild relatives, containing the gene(s of interest. Chromosome engineering has been successfully applied to introduce into wheat genes/QTL for resistance to biotic and abiotic stresses, quality attributes, and even yield-related traits. In recent years, a substantial upsurge in effective alien gene exploitation for wheat improvement has come from modern technologies, including use of molecular markers, molecular cytogenetic techniques, and sequencing, which have greatly expanded our knowledge and ability to finely manipulate wheat and alien genomes. Examples will be provided of various types of stable introgressions, including pyramiding of different alien genes/QTL, into the background of bread and durum wheat genotypes, representing valuable materials for both species to respond to the needed novelty in current and future breeding programs. Challenging contexts, such as that inherent to the 4x nature of durum wheat when compared to 6x bread wheat, or

  17. [Structure of the gene pool of ethnic groups from the Altai-Sayan region from data on mitochondrial polymorphism].

    Science.gov (United States)

    Derenko, M V; Denisova, G A; Maliarchuk, B A; Dambueva, I K; Luzina, F A; Lotosh, E A; Dorzhu, Ch M; Karamchakova, O N; Solovenchuk, L L; Zakharov, I A

    2001-10-01

    Using the data on mitochondrial DNA (mtDNA) polymorphism, genetic structures of the four Turkic-speaking ethnic groups of Altai-Sayan highlands, Southern Altaians (Altai-Kizhi), Khakassians, Shorians, and Sojots, were described. Mitochondrial gene pools of the populations examined were characterized by different ratios between Mongoloid (M*, C, D, E, G, A, B, and F) and Caucasoid (H, U, T, J, and K) mtDNA lineages. All the populations studied had a strongly pronounced Mongoloid component, the frequency of which was 88.2% in Sojots, 75.9% in Khakassians, 67.4% in Altaians, and 64.3% in Shorians. Maximum frequency of the Caucasoid component (35.7%) was observed in Shorians. Phylogenetic and statistical analyses of the mtDNA group frequency distribution patterns in the gene pools of the ethnic populations of Altai-Sayan highlands and the adjacent territories showed that the populations of the region fell into three groups. The first group included Khakassians, Tuvinians and Altaians, the second group consisted of Sojots, Buryats, and Mongols, while the third group was composed of Uigurs, Kazakhs, and Kyrgyzes. The isolated position of Shorians among the populations examined can be explained by their different anthropological composition and their presumptive relatedness to Finno-Ugric populations of Siberia.

  18. Demographic history of Canary Islands male gene-pool: replacement of native lineages by European

    Directory of Open Access Journals (Sweden)

    Amorim António

    2009-08-01

    Full Text Available Abstract Background The origin and prevalence of the prehispanic settlers of the Canary Islands has attracted great multidisciplinary interest. However, direct ancient DNA genetic studies on indigenous and historical 17th–18th century remains, using mitochondrial DNA as a female marker, have only recently been possible. In the present work, the analysis of Y-chromosome polymorphisms in the same samples, has shed light on the way the European colonization affected male and female Canary Island indigenous genetic pools, from the conquest to present-day times. Results Autochthonous (E-M81 and prominent (E-M78 and J-M267 Berber Y-chromosome lineages were detected in the indigenous remains, confirming a North West African origin for their ancestors which confirms previous mitochondrial DNA results. However, in contrast with their female lineages, which have survived in the present-day population since the conquest with only a moderate decline, the male indigenous lineages have dropped constantly being substituted by European lineages. Male and female sub-Saharan African genetic inputs were also detected in the Canary population, but their frequencies were higher during the 17th–18th centuries than today. Conclusion The European colonization of the Canary Islands introduced a strong sex-biased change in the indigenous population in such a way that indigenous female lineages survived in the extant population in a significantly higher proportion than their male counterparts.

  19. Long term repeated prescribed burning increases evenness in the basidiomycete laccase gene pool in forest soils.

    Science.gov (United States)

    Artz, Rebekka R E; Reid, Eileen; Anderson, Ian C; Campbell, Colin D; Cairney, John W G

    2009-03-01

    Repeated prescribed burning alters the biologically labile fraction of nutrients and carbon of soil organic matter (SOM). Using a long-term (30 years) repeated burning experiment where burning has been carried out at a 2- or 4-year frequency, we analysed the effect of prescribed burning on gross potential C turnover rates and phenol oxidase activity in relation to shifts in SOM composition as observed using Fourier-transform infrared spectroscopy. In tandem, we assessed the genetic diversity of basidiomycete laccases. While the overall effect of burning was a decline in phenol oxidase activity, Shannon diversity and evenness of laccases was significantly higher in burned sites. Co-correspondence analysis of SOM composition and laccase operational taxonomic unit frequency data also suggested a strong correlation. While this correlation could indicate that the observed increase in laccase genetic diversity due to burning is due to increased resource diversity, a temporal replacement of the most abundant members of the assembly by an otherwise dormant pool of fungi cannot be excluded. As such, our results fit the intermediate disturbance hypothesis. Effects were stronger in plots burned in 2-year rotations, suggesting that the 4-year burn frequency may be a more sustainable practice to ensure the long-term stability of C cycling in such ecosystems.

  20. More to NAD+ than meets the eye: A regulator of metabolic pools and gene expression in Arabidopsis.

    Science.gov (United States)

    Gakière, Bertrand; Fernie, Alisdair R; Pétriacq, Pierre

    2018-01-05

    Since its discovery more than a century ago, nicotinamide adenine dinucleotide (NAD + ) is recognised as a fascinating cornerstone of cellular metabolism. This ubiquitous energy cofactor plays vital roles in metabolic pathways and regulatory processes, a fact emphasised by the essentiality of a balanced NAD + metabolism for normal plant growth and development. Research on the role of NAD in plants has been predominantly carried out in the model plant Arabidopsis thaliana (Arabidopsis) with emphasis on the redox properties and cellular signalling functions of the metabolite. This review examines the current state of knowledge concerning how NAD can regulate both metabolic pools and gene expression in Arabidopsis. Particular focus is placed on recent studies highlighting the complexity of metabolic regulations involving NAD, more particularly in the mitochondrial compartment, and of signalling roles with respect to interactions with environmental fluctuations most specifically those involving plant immunity. Copyright © 2018 Elsevier Inc. All rights reserved.

  1. Resistance gene pool to co-trimoxazole in non-susceptible Nocardia strains.

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    Sylvia eValdezate

    2015-04-01

    Full Text Available The soil-borne pathogen Nocardia spp. causes severe cutaneous, pulmonary and central nervous system infections. Against them, co-trimoxazole (SXT constitutes the mainstay of antimicrobial therapy. However, some Nocardia strains show resistance to SXT, but the underlying genetic basis is unknown. We investigated the presence of genetic resistance determinants and class 1-3 integrons in 76 SXT-resistant Nocardia strains by PCR and sequencing. By E-test, these clinical strains showed SXT MICs of ≥32:608 mg/L (ratio of 1:19 for trimethoprim: sulfamethoxazole. They belonged to 12 species, being the main representatives N. farcinica (32%, followed by N. flavorosea (6.5%, N. nova (11.8%, N. carnea (10.5%, N. transvalensis (10.5% and Nocardia spp. (6.5%. The prevalence of resistance genes in the SXT-resistant strains was as follows: sul1 and sul2 93.4% and 78.9% respectively, dfrA(S1 14.7%, blaTEM-1 and blaZ 2.6% and 2.6% respectively, VIM-2 1.3%, aph(3´-IIIa 40.8%, ermA, ermB, mefA and msrD 2.6%, 77.6%, 14.4%, and 5.2% respectively, and tet(O, tet(M, and tet(L 48.6%, 25.0% and 3.9% respectively. Detected amino acid changes in GyrA were not related to fluoroquinolone resistance, but probably linked to species polymorphism. Class 1 and 3 integrons were found in 93.42% and 56.57% strains, respectively. Class 2 integrons and sul3 genes were not detected. Other mechanisms, different than dfrA(S1, dfrD, dfrF, dfrG and dfrK, could explain the strong trimethoprim resistance shown by the other 64 strains. For first time, resistance determinants commonly found in clinically important bacteria were detected in Nocardia spp. sul1, sul2, erm(B and tet(O were the most prevalent in the SXT-resistant strains. The similarity in their resistome could be due to a common genetic platform, in which these determinants are co-transferred

  2. Comparison of individual and pooled samples for quantification of antimicrobial resistance genes in swine feces by high-throughput qPCR

    DEFF Research Database (Denmark)

    Clasen, Julie; Mellerup, Anders; Olsen, J. E.

    2015-01-01

    There is a considerable societal interest in the careful monitoring of antimicrobial resistance (AMR) levels in human and animal populations. Sampling and data analysis can be both costly and time consuming. Optimization of sample pooling procedures is therefore important to reduce costs...... and analysis times. The objective of this study was to estimate how many individual fecal samples are needed to pool to get a representative sample for quantification of AMR-genes in a Danish pig herd. 20 individual fecal samples were collected from one section in a Danish pig herd. One to five rectal fecal...... samples were taken from each pen with respect to the number of pigs in the pen. A total of 48 pools were made of increasing number of individual samples. The levels of 9 different AMR-genes were quantified using dynamic qPCR arrays on the BioMark HD system(Fluidigm®).DNA was extracted using the Maxwell...

  3. Cash pooling

    OpenAIRE

    Lozovaya, Karina

    2009-01-01

    This work makes a mention of cash management. At next chapter describes two most known theoretical models of cash management -- Baumol Model and Miller-Orr Model. Principal part of work is about cash pooling, types of cash pooling, cash pooling at Czech Republic and influence of cash pooling over accounting and taxes.

  4. Microdiversification of a Pelagic Polynucleobacter Species Is Mainly Driven by Acquisition of Genomic Islands from a Partially Interspecific Gene Pool

    Science.gov (United States)

    Schmidt, Johanna; Jezberová, Jitka; Koll, Ulrike; Hahn, Martin W.

    2016-01-01

    ABSTRACT Microdiversification of a planktonic freshwater bacterium was studied by comparing 37 Polynucleobacter asymbioticus strains obtained from three geographically separated sites in the Austrian Alps. Genome comparison of nine strains revealed a core genome of 1.8 Mb, representing 81% of the average genome size. Seventy-five percent of the remaining flexible genome is clustered in genomic islands (GIs). Twenty-four genomic positions could be identified where GIs are potentially located. These positions are occupied strain specifically from a set of 28 GI variants, classified according to similarities in their gene content. One variant, present in 62% of the isolates, encodes a pathway for the degradation of aromatic compounds, and another, found in 78% of the strains, contains an operon for nitrate assimilation. Both variants were shown in ecophysiological tests to be functional, thus providing the potential for microniche partitioning. In addition, detected interspecific horizontal exchange of GIs indicates a large gene pool accessible to Polynucleobacter species. In contrast to core genes, GIs are spread more successfully across spatially separated freshwater habitats. The mobility and functional diversity of GIs allow for rapid evolution, which may be a key aspect for the ubiquitous occurrence of Polynucleobacter bacteria. IMPORTANCE Assessing the ecological relevance of bacterial diversity is a key challenge for current microbial ecology. The polyphasic approach which was applied in this study, including targeted isolation of strains, genome analysis, and ecophysiological tests, is crucial for the linkage of genetic and ecological knowledge. Particularly great importance is attached to the high number of closely related strains which were investigated, represented by genome-wide average nucleotide identities (ANI) larger than 97%. The extent of functional diversification found on this narrow phylogenetic scale is compelling. Moreover, the transfer of

  5. Identification of gene pools used in restoration and conservation by chloroplast microsatellite markers in Iberian pine species

    Directory of Open Access Journals (Sweden)

    Enrique Hernández-Tecles

    2017-10-01

    Full Text Available Aim of study: To contribute to the characterization of the origin of material used in afforestation, restoration or conservation activities by using Cp-SSR markers. Area of study: We used information from the natural range of Iberian pines, from Spain. Materials and methods: We used Iberian pines as an example to undertook gene pool characterization based on a wide Iberian sample of 97 populations from five Pinus species (Pinus halepensis, Pinus pinaster, Pinus nigra, Pinus sylvestris and Pinus uncinata. Haplotypes from each analyzed tree (derived from nine chloroplast microsatellites markers in P. halepensis and six in the rest of the species were obtained. Based on this information we subdivided each species in regions (considering both genetic structure and its application in afforestation, restoration and conservation programs and tested the assignation of populations to the different groups based on the genetic distance among samples. Main results: The rate of successful identification of populations among the different species was very high (> 94 % for P. nigra, P. sylvestris and P. uncinata, high (81 % for P. pinaster, and low (< 65 % for P. halepensis. Research highlights: Chloroplast DNA markers from extensive population datasets can be used to assign the origin of the forest reproductive material in some pine species.

  6. Identification of gene pools used in restoration and conservation by chloroplast microsatellite markers in Iberian pine species

    International Nuclear Information System (INIS)

    Hernández-Tecles, Enrique; De las Heras, Jorge; Lorenzo, Zaida; Navascués, Miguel; Alia, Ricardo

    2017-01-01

    Aim of study: To contribute to the characterization of the origin of material used in afforestation, restoration or conservation activities by using Cp-SSR markers. Area of study: We used information from the natural range of Iberian pines, from Spain. Materials and methods: We used Iberian pines as an example to undertook gene pool characterization based on a wide Iberian sample of 97 populations from five Pinus species (Pinus halepensis, Pinus pinaster, Pinus nigra, Pinus sylvestris and Pinus uncinata). Haplotypes from each analyzed tree (derived from nine chloroplast microsatellites markers in P. halepensis and six in the rest of the species) were obtained. Based on this information we subdivided each species in regions (considering both genetic structure and its application in afforestation, restoration and conservation programs) and tested the assignation of populations to the different groups based on the genetic distance among samples. Main results: The rate of successful identification of populations among the different species was very high (> 94 %) for P. nigra, P. sylvestris and P. uncinata, high (81 %) for P. pinaster, and low (< 65 %) for P. halepensis. Research highlights: Chloroplast DNA markers from extensive population datasets can be used to assign the origin of the forest reproductive material in some pine species.

  7. Identification of gene pools used in restoration and conservation by chloroplast microsatellite markers in Iberian pine species

    Energy Technology Data Exchange (ETDEWEB)

    Hernández-Tecles, Enrique; De las Heras, Jorge; Lorenzo, Zaida; Navascués, Miguel; Alia, Ricardo

    2017-11-01

    Aim of study: To contribute to the characterization of the origin of material used in afforestation, restoration or conservation activities by using Cp-SSR markers. Area of study: We used information from the natural range of Iberian pines, from Spain. Materials and methods: We used Iberian pines as an example to undertook gene pool characterization based on a wide Iberian sample of 97 populations from five Pinus species (Pinus halepensis, Pinus pinaster, Pinus nigra, Pinus sylvestris and Pinus uncinata). Haplotypes from each analyzed tree (derived from nine chloroplast microsatellites markers in P. halepensis and six in the rest of the species) were obtained. Based on this information we subdivided each species in regions (considering both genetic structure and its application in afforestation, restoration and conservation programs) and tested the assignation of populations to the different groups based on the genetic distance among samples. Main results: The rate of successful identification of populations among the different species was very high (> 94 %) for P. nigra, P. sylvestris and P. uncinata, high (81 %) for P. pinaster, and low (< 65 %) for P. halepensis. Research highlights: Chloroplast DNA markers from extensive population datasets can be used to assign the origin of the forest reproductive material in some pine species.

  8. When stress predicts a shrinking gene pool, trading early reproduction for longevity can increase fitness, even with lower fecundity.

    Directory of Open Access Journals (Sweden)

    William C Ratcliff

    2009-06-01

    Full Text Available Stresses like dietary restriction or various toxins increase lifespan in taxa as diverse as yeast, Caenorhabditis elegans, Drosophila and rats, by triggering physiological responses that also tend to delay reproduction. Food odors can reverse the effects of dietary restriction, showing that key mechanisms respond to information, not just resources. Such environmental cues can predict population trends, not just individual prospects for survival and reproduction. When population size is increasing, each offspring produced earlier makes a larger proportional contribution to the gene pool, but the reverse is true when population size is declining.We show mathematically that natural selection can favor facultative delay in reproduction when environmental cues predict a decrease in total population size, even if lifetime fecundity decreases with delay. We also show that increased reproduction from waiting for better conditions does not increase fitness (proportional representation when the whole population benefits similarly.We conclude that the beneficial effects of stress on longevity (hormesis in diverse taxa are a side-effect of delaying reproduction in response to environmental cues that population size is likely to decrease. The reversal by food odors of the effects of dietary restriction can be explained as a response to information that population size is less likely to decrease, reducing the chance that delaying reproduction will increase fitness.

  9. Exciting Pools

    Science.gov (United States)

    Wright, Bradford L.

    1975-01-01

    Advocates the creation of swimming pool oscillations as part of a general investigation of mechanical oscillations. Presents the equations, procedure for deriving the slosh modes, and methods of period estimation for exciting swimming pool oscillations. (GS)

  10. Pooled Sequencing of 531 Genes in Inflammatory Bowel Disease Identifies an Associated Rare Variant in BTNL2 and Implicates Other Immune Related Genes

    Science.gov (United States)

    Prescott, Natalie J.; Lehne, Benjamin; Stone, Kristina; Lee, James C.; Taylor, Kirstin; Knight, Jo; Papouli, Efterpi; Mirza, Muddassar M.; Simpson, Michael A.; Spain, Sarah L.; Lu, Grace; Fraternali, Franca; Bumpstead, Suzannah J.; Gray, Emma; Amar, Ariella; Bye, Hannah; Green, Peter; Chung-Faye, Guy; Hayee, Bu’Hussain; Pollok, Richard; Satsangi, Jack; Parkes, Miles; Barrett, Jeffrey C.; Mansfield, John C.; Sanderson, Jeremy; Lewis, Cathryn M.; Weale, Michael E.; Schlitt, Thomas; Mathew, Christopher G.

    2015-01-01

    The contribution of rare coding sequence variants to genetic susceptibility in complex disorders is an important but unresolved question. Most studies thus far have investigated a limited number of genes from regions which contain common disease associated variants. Here we investigate this in inflammatory bowel disease by sequencing the exons and proximal promoters of 531 genes selected from both genome-wide association studies and pathway analysis in pooled DNA panels from 474 cases of Crohn’s disease and 480 controls. 80 variants with evidence of association in the sequencing experiment or with potential functional significance were selected for follow up genotyping in 6,507 IBD cases and 3,064 population controls. The top 5 disease associated variants were genotyped in an extension panel of 3,662 IBD cases and 3,639 controls, and tested for association in a combined analysis of 10,147 IBD cases and 7,008 controls. A rare coding variant p.G454C in the BTNL2 gene within the major histocompatibility complex was significantly associated with increased risk for IBD (p = 9.65x10−10, OR = 2.3[95% CI = 1.75–3.04]), but was independent of the known common associated CD and UC variants at this locus. Rare (T) or decreased risk (IL12B p.V298F, and NICN p.H191R) of IBD. These results provide additional insights into the involvement of the inhibition of T cell activation in the development of both sub-phenotypes of inflammatory bowel disease. We suggest that although rare coding variants may make a modest overall contribution to complex disease susceptibility, they can inform our understanding of the molecular pathways that contribute to pathogenesis. PMID:25671699

  11. Domestication of the neotropical tree Chrysophyllum cainito from a geographically limited yet genetically diverse gene pool in Panama.

    Science.gov (United States)

    Petersen, Jennifer J; Parker, Ingrid M; Potter, Daniel

    2014-03-01

    Species in the early stages of domestication, in which wild and cultivated forms co-occur, provide important opportunities to develop and test hypotheses about the origins of crop species. Chrysophyllum cainito (Sapotaceae), the star apple or caimito, is a semidomesticated tree widely cultivated for its edible fruits; it is known to be native to the neotropics, but its precise geographic origins have not been firmly established. Here, we report results of microsatellite marker analyses supporting the hypothesis that the center of domestication for caimito was the Isthmus of Panama, a region in which few crop species are believed to have originated, despite its importance as a crossroads for the dispersal of domesticated plants between North and South America. Our data suggest that caimito was domesticated in a geographically restricted area while incorporating a diverse gene pool. These results refute the generally accepted Antillean origin of caimito, as well as alternative hypotheses that the species was domesticated independently in the two areas or over a broad geographic range including both. Human-mediated dispersal from Panama to the north and east was accompanied by strong reductions in both genotypic and phenotypic diversity. Within Panama, cultivated and wild trees show little neutral genetic divergence, in contrast to striking phenotypic differentiation in fruit and seed traits. In addition to providing a rare example of data that support the hypothesis of a narrow geographic origin on the Isthmus of Panama for a now widespread cultivated plant species, this study is one of the first investigations of the origins of an edible species of the large pantropical family Sapotaceae.

  12. The contribution of Escherichia coli from human and animal sources to the integron gene pool in coastal waters

    Science.gov (United States)

    Moura, Alexandra; Araújo, Susana; Alves, Marta S.; Henriques, Isabel; Pereira, Anabela; Correia, António C. M.

    2014-01-01

    To understand the contribution of animal- and human-derived fecal pollution sources in shaping integron prevalence and diversity in beach waters, 414 Escherichia coli strains were collected from beach waters (BW, n = 166), seagull feces (SF, n = 179), and wastewaters (WW, n = 69), on the World Biosphere Reserve of the Berlenga Island, Portugal. Statistical differences were found between the prevalence of integrons in BW (21%) and WW (10%), but not between BW and SF (19%). The majority of integrase-positive (intI+)-strains affiliated to commensal phylogroups B1 (37%), A0 (24%), and A1 (20%). Eighteen different gene cassette arrays were detected, most of them coding for resistances to aminoglycosides, trimethoprim, chloramphenicol, and quaternary ammonia compounds. Common arrays were found among strains from different sources. Multi-resistance to three or more different classes of antibiotics was observed in 89, 82, and 57% of intI+-strains from BW, SF and WW, respectively. Plasmids were detected in 79% of strains (60/76) revealing a high diversity of replicons in all sources, mostly belonging to IncF (Frep, FIA, and FIB subgroups), IncI1, IncN, IncY, and IncK incompatibility groups. In 20% (15/76) of strains, integrons were successfully mobilized through conjugation to E. coli CV601. Results obtained support the existence of a diverse integron pool in the E. coli strains from this coastal environment, associated with different resistance traits and plasmid incompatibility groups, mainly shaped by animal fecal pollution inputs. These findings underscore the role of wild life in dissemination of integrons and antibiotic resistance traits in natural environments. PMID:25161650

  13. Mining a database of single amplified genomes from Red Sea brine pool extremophiles—improving reliability of gene function prediction using a profile and pattern matching algorithm (PPMA)

    Science.gov (United States)

    Grötzinger, Stefan W.; Alam, Intikhab; Ba Alawi, Wail; Bajic, Vladimir B.; Stingl, Ulrich; Eppinger, Jörg

    2014-01-01

    Reliable functional annotation of genomic data is the key-step in the discovery of novel enzymes. Intrinsic sequencing data quality problems of single amplified genomes (SAGs) and poor homology of novel extremophile's genomes pose significant challenges for the attribution of functions to the coding sequences identified. The anoxic deep-sea brine pools of the Red Sea are a promising source of novel enzymes with unique evolutionary adaptation. Sequencing data from Red Sea brine pool cultures and SAGs are annotated and stored in the Integrated Data Warehouse of Microbial Genomes (INDIGO) data warehouse. Low sequence homology of annotated genes (no similarity for 35% of these genes) may translate into false positives when searching for specific functions. The Profile and Pattern Matching (PPM) strategy described here was developed to eliminate false positive annotations of enzyme function before progressing to labor-intensive hyper-saline gene expression and characterization. It utilizes InterPro-derived Gene Ontology (GO)-terms (which represent enzyme function profiles) and annotated relevant PROSITE IDs (which are linked to an amino acid consensus pattern). The PPM algorithm was tested on 15 protein families, which were selected based on scientific and commercial potential. An initial list of 2577 enzyme commission (E.C.) numbers was translated into 171 GO-terms and 49 consensus patterns. A subset of INDIGO-sequences consisting of 58 SAGs from six different taxons of bacteria and archaea were selected from six different brine pool environments. Those SAGs code for 74,516 genes, which were independently scanned for the GO-terms (profile filter) and PROSITE IDs (pattern filter). Following stringent reliability filtering, the non-redundant hits (106 profile hits and 147 pattern hits) are classified as reliable, if at least two relevant descriptors (GO-terms and/or consensus patterns) are present. Scripts for annotation, as well as for the PPM algorithm, are available

  14. Mining a database of single amplified genomes from Red Sea brine pool extremophiles – Improving reliability of gene function prediction using a profile and pattern matching algorithm (PPMA

    Directory of Open Access Journals (Sweden)

    Stefan Wolfgang Grötzinger

    2014-04-01

    Full Text Available Reliable functional annotation of genomic data is the key-step in the discovery of novel enzymes. Intrinsic sequencing data quality problems of single amplified genomes (SAGs and poor homology of novel extremophile’s genomes pose significant challenges for the attribution of functions to the coding sequences identified. The anoxic deep-sea brine pools of the Red Sea are a promising source of novel enzymes with unique evolutionary adaptation. Sequencing data from Red Sea brine pool cultures and SAGs are annotated and stored in the INDIGO data warehouse. Low sequence homology of annotated genes (no similarity for 35% of these genes may translate into false positives when searching for specific functions. The Profile & Pattern Matching (PPM strategy described here was developed to eliminate false positive annotations of enzyme function before progressing to labor-intensive hyper-saline gene expression and characterization. It utilizes InterPro-derived Gene Ontology (GO-terms (which represent enzyme function profiles and annotated relevant PROSITE IDs (which are linked to an amino acid consensus pattern. The PPM algorithm was tested on 15 protein families, which were selected based on scientific and commercial potential. An initial list of 2,577 E.C. numbers was translated into 171 GO-terms and 49 consensus patterns. A subset of INDIGO-sequences consisting of 58 SAGs from six different taxons of bacteria and archaea were selected from 6 different brine pool environments. Those SAGs code for 74,516 genes, which were independently scanned for the GO-terms (profile filter and PROSITE IDs (pattern filter. Following stringent reliability filtering, the non-redundant hits (106 profile hits and 147 pattern hits are classified as reliable, if at least two relevant descriptors (GO-terms and/or consensus patterns are present. Scripts for annotation, as well as for the PPM algorithm, are available through the INDIGO website.

  15. Mining a database of single amplified genomes from Red Sea brine pool extremophiles-improving reliability of gene function prediction using a profile and pattern matching algorithm (PPMA).

    KAUST Repository

    Grötzinger, Stefan W.

    2014-04-07

    Reliable functional annotation of genomic data is the key-step in the discovery of novel enzymes. Intrinsic sequencing data quality problems of single amplified genomes (SAGs) and poor homology of novel extremophile\\'s genomes pose significant challenges for the attribution of functions to the coding sequences identified. The anoxic deep-sea brine pools of the Red Sea are a promising source of novel enzymes with unique evolutionary adaptation. Sequencing data from Red Sea brine pool cultures and SAGs are annotated and stored in the Integrated Data Warehouse of Microbial Genomes (INDIGO) data warehouse. Low sequence homology of annotated genes (no similarity for 35% of these genes) may translate into false positives when searching for specific functions. The Profile and Pattern Matching (PPM) strategy described here was developed to eliminate false positive annotations of enzyme function before progressing to labor-intensive hyper-saline gene expression and characterization. It utilizes InterPro-derived Gene Ontology (GO)-terms (which represent enzyme function profiles) and annotated relevant PROSITE IDs (which are linked to an amino acid consensus pattern). The PPM algorithm was tested on 15 protein families, which were selected based on scientific and commercial potential. An initial list of 2577 enzyme commission (E.C.) numbers was translated into 171 GO-terms and 49 consensus patterns. A subset of INDIGO-sequences consisting of 58 SAGs from six different taxons of bacteria and archaea were selected from six different brine pool environments. Those SAGs code for 74,516 genes, which were independently scanned for the GO-terms (profile filter) and PROSITE IDs (pattern filter). Following stringent reliability filtering, the non-redundant hits (106 profile hits and 147 pattern hits) are classified as reliable, if at least two relevant descriptors (GO-terms and/or consensus patterns) are present. Scripts for annotation, as well as for the PPM algorithm, are available

  16. Adaptive genomic divergence under high gene flow between freshwater and brackish-water ecotypes of prickly sculpin (Cottus asper) revealed by Pool-Seq.

    Science.gov (United States)

    Dennenmoser, Stefan; Vamosi, Steven M; Nolte, Arne W; Rogers, Sean M

    2017-01-01

    Understanding the genomic basis of adaptive divergence in the presence of gene flow remains a major challenge in evolutionary biology. In prickly sculpin (Cottus asper), an abundant euryhaline fish in northwestern North America, high genetic connectivity among brackish-water (estuarine) and freshwater (tributary) habitats of coastal rivers does not preclude the build-up of neutral genetic differentiation and emergence of different life history strategies. Because these two habitats present different osmotic niches, we predicted high genetic differentiation at known teleost candidate genes underlying salinity tolerance and osmoregulation. We applied whole-genome sequencing of pooled DNA samples (Pool-Seq) to explore adaptive divergence between two estuarine and two tributary habitats. Paired-end sequence reads were mapped against genomic contigs of European Cottus, and the gene content of candidate regions was explored based on comparisons with the threespine stickleback genome. Genes showing signals of repeated differentiation among brackish-water and freshwater habitats included functions such as ion transport and structural permeability in freshwater gills, which suggests that local adaptation to different osmotic niches might contribute to genomic divergence among habitats. Overall, the presence of both repeated and unique signatures of differentiation across many loci scattered throughout the genome is consistent with polygenic adaptation from standing genetic variation and locally variable selection pressures in the early stages of life history divergence. © 2016 John Wiley & Sons Ltd.

  17. Pooled Resequencing of 122 Ulcerative Colitis Genes in a Large Dutch Cohort Suggests Population-Specific Associations of Rare Variants in MUC2.

    Science.gov (United States)

    Visschedijk, Marijn C; Alberts, Rudi; Mucha, Soren; Deelen, Patrick; de Jong, Dirk J; Pierik, Marieke; Spekhorst, Lieke M; Imhann, Floris; van der Meulen-de Jong, Andrea E; van der Woude, C Janneke; van Bodegraven, Adriaan A; Oldenburg, Bas; Löwenberg, Mark; Dijkstra, Gerard; Ellinghaus, David; Schreiber, Stefan; Wijmenga, Cisca; Rivas, Manuel A; Franke, Andre; van Diemen, Cleo C; Weersma, Rinse K

    2016-01-01

    Genome-wide association studies have revealed several common genetic risk variants for ulcerative colitis (UC). However, little is known about the contribution of rare, large effect genetic variants to UC susceptibility. In this study, we performed a deep targeted re-sequencing of 122 genes in Dutch UC patients in order to investigate the contribution of rare variants to the genetic susceptibility to UC. The selection of genes consists of 111 established human UC susceptibility genes and 11 genes that lead to spontaneous colitis when knocked-out in mice. In addition, we sequenced the promoter regions of 45 genes where known variants exert cis-eQTL-effects. Targeted pooled re-sequencing was performed on DNA of 790 Dutch UC cases. The Genome of the Netherlands project provided sequence data of 500 healthy controls. After quality control and prioritization based on allele frequency and pathogenicity probability, follow-up genotyping of 171 rare variants was performed on 1021 Dutch UC cases and 1166 Dutch controls. Single-variant association and gene-based analyses identified an association of rare variants in the MUC2 gene with UC. The associated variants in the Dutch population could not be replicated in a German replication cohort (1026 UC cases, 3532 controls). In conclusion, this study has identified a putative role for MUC2 on UC susceptibility in the Dutch population and suggests a population-specific contribution of rare variants to UC.

  18. Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species

    Directory of Open Access Journals (Sweden)

    Bolhuis Henk

    2006-07-01

    Full Text Available Abstract Background Mature saturated brine (crystallizers communities are largely dominated (>80% of cells by the square halophilic archaeon "Haloquadratum walsbyi". The recent cultivation of the strain HBSQ001 and thesequencing of its genome allows comparison with the metagenome of this taxonomically simplified environment. Similar studies carried out in other extreme environments have revealed very little diversity in gene content among the cell lineages present. Results The metagenome of the microbial community of a crystallizer pond has been analyzed by end sequencing a 2000 clone fosmid library and comparing the sequences obtained with the genome sequence of "Haloquadratum walsbyi". The genome of the sequenced strain was retrieved nearly complete within this environmental DNA library. However, many ORF's that could be ascribed to the "Haloquadratum" metapopulation by common genome characteristics or scaffolding to the strain genome were not present in the specific sequenced isolate. Particularly, three regions of the sequenced genome were associated with multiple rearrangements and the presence of different genes from the metapopulation. Many transposition and phage related genes were found within this pool which, together with the associated atypical GC content in these areas, supports lateral gene transfer mediated by these elements as the most probable genetic cause of this variability. Additionally, these sequences were highly enriched in putative regulatory and signal transduction functions. Conclusion These results point to a large pan-genome (total gene repertoire of the genus/species even in this highly specialized extremophile and at a single geographic location. The extensive gene repertoire is what might be expected of a population that exploits a diverse nutrient pool, resulting from the degradation of biomass produced at lower salinities.

  19. Saturation of an intra-gene pool linkage map: towards a unified consensus linkage map for fine mapping and synteny analysis in common bean.

    Science.gov (United States)

    Galeano, Carlos H; Fernandez, Andrea C; Franco-Herrera, Natalia; Cichy, Karen A; McClean, Phillip E; Vanderleyden, Jos; Blair, Matthew W

    2011-01-01

    Map-based cloning and fine mapping to find genes of interest and marker assisted selection (MAS) requires good genetic maps with reproducible markers. In this study, we saturated the linkage map of the intra-gene pool population of common bean DOR364 × BAT477 (DB) by evaluating 2,706 molecular markers including SSR, SNP, and gene-based markers. On average the polymorphism rate was 7.7% due to the narrow genetic base between the parents. The DB linkage map consisted of 291 markers with a total map length of 1,788 cM. A consensus map was built using the core mapping populations derived from inter-gene pool crosses: DOR364 × G19833 (DG) and BAT93 × JALO EEP558 (BJ). The consensus map consisted of a total of 1,010 markers mapped, with a total map length of 2,041 cM across 11 linkage groups. On average, each linkage group on the consensus map contained 91 markers of which 83% were single copy markers. Finally, a synteny analysis was carried out using our highly saturated consensus maps compared with the soybean pseudo-chromosome assembly. A total of 772 marker sequences were compared with the soybean genome. A total of 44 syntenic blocks were identified. The linkage group Pv6 presented the most diverse pattern of synteny with seven syntenic blocks, and Pv9 showed the most consistent relations with soybean with just two syntenic blocks. Additionally, a co-linear analysis using common bean transcript map information against soybean coding sequences (CDS) revealed the relationship with 787 soybean genes. The common bean consensus map has allowed us to map a larger number of markers, to obtain a more complete coverage of the common bean genome. Our results, combined with synteny relationships provide tools to increase marker density in selected genomic regions to identify closely linked polymorphic markers for indirect selection, fine mapping or for positional cloning.

  20. PDA: Pooled DNA analyzer

    Directory of Open Access Journals (Sweden)

    Lin Chin-Yu

    2006-04-01

    Full Text Available Abstract Background Association mapping using abundant single nucleotide polymorphisms is a powerful tool for identifying disease susceptibility genes for complex traits and exploring possible genetic diversity. Genotyping large numbers of SNPs individually is performed routinely but is cost prohibitive for large-scale genetic studies. DNA pooling is a reliable and cost-saving alternative genotyping method. However, no software has been developed for complete pooled-DNA analyses, including data standardization, allele frequency estimation, and single/multipoint DNA pooling association tests. This motivated the development of the software, 'PDA' (Pooled DNA Analyzer, to analyze pooled DNA data. Results We develop the software, PDA, for the analysis of pooled-DNA data. PDA is originally implemented with the MATLAB® language, but it can also be executed on a Windows system without installing the MATLAB®. PDA provides estimates of the coefficient of preferential amplification and allele frequency. PDA considers an extended single-point association test, which can compare allele frequencies between two DNA pools constructed under different experimental conditions. Moreover, PDA also provides novel chromosome-wide multipoint association tests based on p-value combinations and a sliding-window concept. This new multipoint testing procedure overcomes a computational bottleneck of conventional haplotype-oriented multipoint methods in DNA pooling analyses and can handle data sets having a large pool size and/or large numbers of polymorphic markers. All of the PDA functions are illustrated in the four bona fide examples. Conclusion PDA is simple to operate and does not require that users have a strong statistical background. The software is available at http://www.ibms.sinica.edu.tw/%7Ecsjfann/first%20flow/pda.htm.

  1. Sequencing of a QTL-rich region of the Theobroma cacao genome using pooled BACs and the identification of trait specific candidate genes

    Directory of Open Access Journals (Sweden)

    Blackmon Barbara P

    2011-07-01

    Full Text Available Abstract Background BAC-based physical maps provide for sequencing across an entire genome or a selected sub-genomic region of biological interest. Such a region can be approached with next-generation whole-genome sequencing and assembly as if it were an independent small genome. Using the minimum tiling path as a guide, specific BAC clones representing the prioritized genomic interval are selected, pooled, and used to prepare a sequencing library. Results This pooled BAC approach was taken to sequence and assemble a QTL-rich region, of ~3 Mbp and represented by twenty-seven BACs, on linkage group 5 of the Theobroma cacao cv. Matina 1-6 genome. Using various mixtures of read coverages from paired-end and linear 454 libraries, multiple assemblies of varied quality were generated. Quality was assessed by comparing the assembly of 454 reads with a subset of ten BACs individually sequenced and assembled using Sanger reads. A mixture of reads optimal for assembly was identified. We found, furthermore, that a quality assembly suitable for serving as a reference genome template could be obtained even with a reduced depth of sequencing coverage. Annotation of the resulting assembly revealed several genes potentially responsible for three T. cacao traits: black pod disease resistance, bean shape index, and pod weight. Conclusions Our results, as with other pooled BAC sequencing reports, suggest that pooling portions of a minimum tiling path derived from a BAC-based physical map is an effective method to target sub-genomic regions for sequencing. While we focused on a single QTL region, other QTL regions of importance could be similarly sequenced allowing for biological discovery to take place before a high quality whole-genome assembly is completed.

  2. Polymorphisms in Genes of Relevance for Oestrogen and Oxytocin Pathways and Risk of Barrett's Oesophagus and Oesophageal Adenocarcinoma: A Pooled Analysis from the BEACON Consortium.

    Directory of Open Access Journals (Sweden)

    Katarina Lagergren

    Full Text Available The strong male predominance in oesophageal adenocarcinoma (OAC and Barrett's oesophagus (BO continues to puzzle. Hormonal influence, e.g. oestrogen or oxytocin, might contribute.This genetic-epidemiological study pooled 14 studies from three continents, Australia, Europe, and North America. Polymorphisms in 3 key genes coding for the oestrogen pathway (receptor alpha (ESR1, receptor beta (ESR2, and aromatase (CYP19A1, and 3 key genes of the oxytocin pathway (the oxytocin receptor (OXTR, oxytocin protein (OXT, and cyclic ADP ribose hydrolase glycoprotein (CD38, were analysed using a gene-based approach, versatile gene-based test association study (VEGAS.Among 1508 OAC patients, 2383 BO patients, and 2170 controls, genetic variants within ESR1 were associated with BO in males (p = 0.0058 and an increased risk of OAC and BO combined in males (p = 0.0023. Genetic variants within OXTR were associated with an increased risk of BO in both sexes combined (p = 0.0035 and in males (p = 0.0012. We followed up these suggestive findings in a further smaller data set, but found no replication. There were no significant associations between the other 4 genes studied and risk of OAC, BO, separately on in combination, in males and females combined or in males only.Genetic variants in the oestrogen receptor alpha and the oxytocin receptor may be associated with an increased risk of BO or OAC, but replication in other large samples are needed.

  3. Polymorphisms in Genes of Relevance for Oestrogen and Oxytocin Pathways and Risk of Barrett's Oesophagus and Oesophageal Adenocarcinoma: A Pooled Analysis from the BEACON Consortium.

    Science.gov (United States)

    Lagergren, Katarina; Ek, Weronica E; Levine, David; Chow, Wong-Ho; Bernstein, Leslie; Casson, Alan G; Risch, Harvey A; Shaheen, Nicholas J; Bird, Nigel C; Reid, Brian J; Corley, Douglas A; Hardie, Laura J; Wu, Anna H; Fitzgerald, Rebecca C; Pharoah, Paul; Caldas, Carlos; Romero, Yvonne; Vaughan, Thomas L; MacGregor, Stuart; Whiteman, David; Westberg, Lars; Nyren, Olof; Lagergren, Jesper

    2015-01-01

    The strong male predominance in oesophageal adenocarcinoma (OAC) and Barrett's oesophagus (BO) continues to puzzle. Hormonal influence, e.g. oestrogen or oxytocin, might contribute. This genetic-epidemiological study pooled 14 studies from three continents, Australia, Europe, and North America. Polymorphisms in 3 key genes coding for the oestrogen pathway (receptor alpha (ESR1), receptor beta (ESR2), and aromatase (CYP19A1)), and 3 key genes of the oxytocin pathway (the oxytocin receptor (OXTR), oxytocin protein (OXT), and cyclic ADP ribose hydrolase glycoprotein (CD38)), were analysed using a gene-based approach, versatile gene-based test association study (VEGAS). Among 1508 OAC patients, 2383 BO patients, and 2170 controls, genetic variants within ESR1 were associated with BO in males (p = 0.0058) and an increased risk of OAC and BO combined in males (p = 0.0023). Genetic variants within OXTR were associated with an increased risk of BO in both sexes combined (p = 0.0035) and in males (p = 0.0012). We followed up these suggestive findings in a further smaller data set, but found no replication. There were no significant associations between the other 4 genes studied and risk of OAC, BO, separately on in combination, in males and females combined or in males only. Genetic variants in the oestrogen receptor alpha and the oxytocin receptor may be associated with an increased risk of BO or OAC, but replication in other large samples are needed.

  4. Molecular genetic studies of natives on Easter Island: evidence of an early European and Amerindian contribution to the Polynesian gene pool.

    Science.gov (United States)

    Lie, B A; Dupuy, B M; Spurkland, A; Fernández-Viña, M A; Hagelberg, E; Thorsby, E

    2007-01-01

    Most archaeological and linguistic evidence suggest a Polynesian origin of the population of Easter Island (Rapanui), and this view has been supported by the identification of Polynesian mitochondrial DNA (mtDNA) polymorphisms in prehistoric skeletal remains. However, some evidence of an early South American contact also exists (the sweet potato, bottle gourd etc.), but genetic studies have so far failed to show an early Amerindian contribution to the gene pool on Easter Island. To address this issue, we analyzed mtDNA and Y chromosome markers and performed high-resolution human leukocyte antigen (HLA) genotyping of DNA harvested from previously collected sera of 48 reputedly nonadmixed native Easter Islanders. All individuals carried mtDNA types and HLA alleles previously found in Polynesia, and most men carried Y chromosome markers of Polynesian origin, providing further evidence of a Polynesian origin of the population of Easter Island. A few individuals carried HLA alleles and/or Y chromosome markers of European origin. More interestingly, some individuals carried the HLA alleles A*0212 and B*3905, which are of typical Amerindian origin. The genealogy of some of the individuals carrying these non-Polynesian HLA alleles and their haplotypic backgrounds suggest an introduction into Easter Island in the early 1800s, or earlier. Thus, there may have been an early European and Amerindian contribution to the Polynesian gene pool of Easter Island.

  5. Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA.

    Science.gov (United States)

    Oates, A C; Wollberg, P; Achen, M G; Wilks, A F

    1998-08-28

    The polymerase chain reaction (PCR), with cDNA as template, has been widely used to identify members of protein families from many species. A major limitation of using cDNA in PCR is that detection of a family member is dependent on temporal and spatial patterns of gene expression. To circumvent this restriction, and in order to develop a technique that is broadly applicable we have tested the use of genomic DNA as PCR template to identify members of protein families in an expression-independent manner. This test involved amplification of DNA encoding protein tyrosine kinase (PTK) genes from the genomes of three animal species that are well known development models; namely, the mouse Mus musculus, the fruit fly Drosophila melanogaster, and the nematode worm Caenorhabditis elegans. Ten PTK genes were identified from the mouse, 13 from the fruit fly, and 13 from the nematode worm. Among these kinases were 13 members of the PTK family that had not been reported previously. Selected PTKs from this screen were shown to be expressed during development, demonstrating that the amplified fragments did not arise from pseudogenes. This approach will be useful for the identification of many novel members of gene families in organisms of agricultural, medical, developmental and evolutionary significance and for analysis of gene families from any species, or biological sample whose habitat precludes the isolation of mRNA. Furthermore, as a tool to hasten the discovery of members of gene families that are of particular interest, this method offers an opportunity to sample the genome for new members irrespective of their expression pattern.

  6. Pool scrubbing

    International Nuclear Information System (INIS)

    Lopez-Jimenez, J.; Herranz, J.; Escudero, M.J.; Espigares, M.M.; Peyres, V.; Polo, J.; Kortz, Ch.; Koch, M.K.; Brockmeier, U.; Unger, H.; Dutton, L.M.C.; Smedley, Ch.; Trow, W.; Jones, A.V.; Bonanni, E.; Calvo, M.; Alonso, A.

    1996-12-01

    The Source Term Project in the Third Frame Work Programme of the European Union Was conducted under and important joined effort on pool scrubbing research. CIEMAT was the Task Manager of the project and several other organizations participated in it: JRC-Ispra, NNC Limited, RUB-NES and UPM. The project was divided into several tasks. A peer review of the models in the pool scrubbing codes SPARC90 and BUSCA-AUG92 was made, considering the different aspects in the hydrodynamic phenomenology, particle retention and fission product vapor abortions. Several dominant risk accident sequences were analyzed with MAAP, SPARC90 and BUSCA-AUG92 codes, and the predictions were compared. A churn-turbulent model was developed for the hydrodynamic behaviour of the pool. Finally, an experimental programme in the PECA facility of CIEMAT was conducted in order to study the decontamination factor under jet injection regime, and the experimental observations were compared with the SPARC and BUSCA codes. (Author)

  7. Collaborative pooled analysis of data on C-reactive protein gene variants and coronary disease: judging causality by Mendelian randomisation

    DEFF Research Database (Denmark)

    Danesh, J.; Hingorani, A.; Wensley, F.

    2008-01-01

    Many prospective studies have reported associations between circulating C-reactive protein (CRP) levels and risk of coronary heart disease (CHD), but causality remains uncertain. Studies of CHD are being conducted that involve measurement of common polymorphisms of the CRP gene known to be associ...

  8. De novo 454 sequencing of barcoded BAC pools for comprehensive gene survey and genome analysis in the complex genome of barley

    Directory of Open Access Journals (Sweden)

    Scholz Uwe

    2009-11-01

    Full Text Available Abstract Background De novo sequencing the entire genome of a large complex plant genome like the one of barley (Hordeum vulgare L. is a major challenge both in terms of experimental feasibility and costs. The emergence and breathtaking progress of next generation sequencing technologies has put this goal into focus and a clone based strategy combined with the 454/Roche technology is conceivable. Results To test the feasibility, we sequenced 91 barcoded, pooled, gene containing barley BACs using the GS FLX platform and assembled the sequences under iterative change of parameters. The BAC assemblies were characterized by N50 of ~50 kb (N80 ~31 kb, N90 ~21 kb and a Q40 of 94%. For ~80% of the clones, the best assemblies consisted of less than 10 contigs at 24-fold mean sequence coverage. Moreover we show that gene containing regions seem to assemble completely and uninterrupted thus making the approach suitable for detecting complete and positionally anchored genes. By comparing the assemblies of four clones to their complete reference sequences generated by the Sanger method, we evaluated the distribution, quality and representativeness of the 454 sequences as well as the consistency and reliability of the assemblies. Conclusion The described multiplex 454 sequencing of barcoded BACs leads to sequence consensi highly representative for the clones. Assemblies are correct for the majority of contigs. Though the resolution of complex repetitive structures requires additional experimental efforts, our approach paves the way for a clone based strategy of sequencing the barley genome.

  9. Microbiota composition, gene pool and its expression in Gir cattle (Bos indicus) rumen under different forage diets using metagenomic and metatranscriptomic approaches.

    Science.gov (United States)

    Pandit, Ramesh J; Hinsu, Ankit T; Patel, Shriram H; Jakhesara, Subhash J; Koringa, Prakash G; Bruno, Fosso; Psifidi, Androniki; Shah, S V; Joshi, Chaitanya G

    2018-03-09

    Zebu (Bos indicus) is a domestic cattle species originating from the Indian subcontinent and now widely domesticated on several continents. In this study, we were particularly interested in understanding the functionally active rumen microbiota of an important Zebu breed, the Gir, under different dietary regimes. Metagenomic and metatranscriptomic data were compared at various taxonomic levels to elucidate the differential microbial population and its functional dynamics in Gir cattle rumen under different roughage dietary regimes. Different proportions of roughage rather than the type of roughage (dry or green) modulated microbiome composition and the expression of its gene pool. Fibre degrading bacteria (i.e. Clostridium, Ruminococcus, Eubacterium, Butyrivibrio, Bacillus and Roseburia) were higher in the solid fraction of rumen (Pcomparison of metagenomic shotgun and metatranscriptomic sequencing appeared to be a much richer source of information compared to conventional metagenomic analysis. Copyright © 2018 Elsevier GmbH. All rights reserved.

  10. Genetic diversity within and between broodstocks of the white shrimp Litopenaeus vannamei (Boone, 1931 (Decapoda, Penaeidae and its implication for the gene pool conservation

    Directory of Open Access Journals (Sweden)

    PD. Freitas

    Full Text Available Genetic variation within and between fifteen closed broodstock lines of the Pacific white shrimp Litopenaeus vannamei, reared at different hatcheries in the Brazilian coast, was assessed by RAPD analysis. Fifty two polymorphic loci were identified when a set of five decamer primers was used in PCR. The genetic diversity analysis within lines evidenced genetic variation loss probably related to bottleneck effects and inbreeding. In addition, the genetic divergence values between the different samples appear to reflect the initial founder composition of such stocks, in some cases, sharing a common origin, suggesting a putative importance of interbreeding for the establishment of genetic improvement programs for these broodstocks. The genetic variation monitoring appears to be helpful to the gene pool conservation of this aquaculture species, mainly if considered its exotic status in Brazil and the current impossibility of new introduction of wild individuals.

  11. Gene expression deregulation in postnatal skeletal muscle of TK2 deficient mice reveals a lower pool of proliferating myogenic progenitor cells.

    Directory of Open Access Journals (Sweden)

    João A Paredes

    Full Text Available Loss of thymidine kinase 2 (TK2 causes a heterogeneous myopathic form of mitochondrial DNA (mtDNA depletion syndrome (MDS in humans that predominantly affects skeletal muscle tissue. In mice, TK2 deficiency also affects several tissues in addition to skeletal muscle, including brain, heart, adipose tissue, kidneys and causes death about 3 weeks after birth. We analysed skeletal muscle and heart muscle tissues of Tk2 knockout mice at postnatal development phase and observed that TK2 deficient pups grew slower and their skeletal muscles appeared significantly underdeveloped, whereas heart was close to normal in size. Both tissues showed mtDNA depletion and mitochondria with altered ultrastructure, as revealed by transmission electron microscopy. Gene expression microarray analysis showed a strong down-regulation of genes involved in cell cycle and cell proliferation in both tissues, suggesting a lower pool of undifferentiated proliferating cells. Analysis of isolated primary myoblasts from Tk2 knockout mice showed slow proliferation, less ability to differentiate and signs of premature senescence, even in absence of mtDNA depletion. Our data demonstrate that TK2 deficiency disturbs myogenic progenitor cells function in postnatal skeletal muscle and we propose this as one of the causes of underdeveloped phenotype and myopathic characteristic of the TK2 deficient mice, in addition to the progressive mtDNA depletion, mitochondrial damage and respiratory chain deficiency in post-mitotic differentiated tissue.

  12. Gene expression deregulation in postnatal skeletal muscle of TK2 deficient mice reveals a lower pool of proliferating myogenic progenitor cells.

    Science.gov (United States)

    Paredes, João A; Zhou, Xiaoshan; Höglund, Stefan; Karlsson, Anna

    2013-01-01

    Loss of thymidine kinase 2 (TK2) causes a heterogeneous myopathic form of mitochondrial DNA (mtDNA) depletion syndrome (MDS) in humans that predominantly affects skeletal muscle tissue. In mice, TK2 deficiency also affects several tissues in addition to skeletal muscle, including brain, heart, adipose tissue, kidneys and causes death about 3 weeks after birth. We analysed skeletal muscle and heart muscle tissues of Tk2 knockout mice at postnatal development phase and observed that TK2 deficient pups grew slower and their skeletal muscles appeared significantly underdeveloped, whereas heart was close to normal in size. Both tissues showed mtDNA depletion and mitochondria with altered ultrastructure, as revealed by transmission electron microscopy. Gene expression microarray analysis showed a strong down-regulation of genes involved in cell cycle and cell proliferation in both tissues, suggesting a lower pool of undifferentiated proliferating cells. Analysis of isolated primary myoblasts from Tk2 knockout mice showed slow proliferation, less ability to differentiate and signs of premature senescence, even in absence of mtDNA depletion. Our data demonstrate that TK2 deficiency disturbs myogenic progenitor cells function in postnatal skeletal muscle and we propose this as one of the causes of underdeveloped phenotype and myopathic characteristic of the TK2 deficient mice, in addition to the progressive mtDNA depletion, mitochondrial damage and respiratory chain deficiency in post-mitotic differentiated tissue.

  13. Determining the optimal number of individual samples to pool for quantification of average herd levels of antimicrobial resistance genes in Danish pig herds using high-throughput qPCR

    DEFF Research Database (Denmark)

    Clasen, Julie; Mellerup, Anders; Olsen, John Elmerdahl

    2016-01-01

    The primary objective of this study was to determine the minimum number of individual fecal samples to pool together in order to obtain a representative sample for herd level quantification of antimicrobial resistance (AMR) genes in a Danish pig herd, using a novel high-throughput qPCR assay...

  14. Hawaii ESI: POOLS (Anchialine Pool Points)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for anchialine pools in Hawaii. Anchialine pools are small, relatively shallow coastal ponds that occur...

  15. Linking the sub-Saharan and West Eurasian gene pools: maternal and paternal heritage of the Tuareg nomads from the African Sahel.

    Science.gov (United States)

    Pereira, Luísa; Cerný, Viktor; Cerezo, María; Silva, Nuno M; Hájek, Martin; Vasíková, Alzbeta; Kujanová, Martina; Brdicka, Radim; Salas, Antonio

    2010-08-01

    The Tuareg presently live in the Sahara and the Sahel. Their ancestors are commonly believed to be the Garamantes of the Libyan Fezzan, ever since it was suggested by authors of antiquity. Biological evidence, based on classical genetic markers, however, indicates kinship with the Beja of Eastern Sudan. Our study of mitochondrial DNA (mtDNA) sequences and Y chromosome SNPs of three different southern Tuareg groups from Mali, Burkina Faso and the Republic of Niger reveals a West Eurasian-North African composition of their gene pool. The data show that certain genetic lineages could not have been introduced into this population earlier than approximately 9000 years ago whereas local expansions establish a minimal date at around 3000 years ago. Some of the mtDNA haplogroups observed in the Tuareg population were involved in the post-Last Glacial Maximum human expansion from Iberian refugia towards both Europe and North Africa. Interestingly, no Near Eastern mtDNA lineages connected with the Neolithic expansion have been observed in our population sample. On the other hand, the Y chromosome SNPs data show that the paternal lineages can very probably be traced to the Near Eastern Neolithic demic expansion towards North Africa, a period that is otherwise concordant with the above-mentioned mtDNA expansion. The time frame for the migration of the Tuareg towards the African Sahel belt overlaps that of early Holocene climatic changes across the Sahara (from the optimal greening approximately 10 000 YBP to the extant aridity beginning at approximately 6000 YBP) and the migrations of other African nomadic peoples in the area.

  16. Genetic heritage of Croatians in the Southeastern European gene pool-Y chromosome analysis of the Croatian continental and Island population.

    Science.gov (United States)

    Šarac, Jelena; Šarić, Tena; Havaš Auguštin, Dubravka; Novokmet, Natalija; Vekarić, Nenad; Mustać, Mate; Grahovac, Blaženka; Kapović, Miljenko; Nevajda, Branimir; Glasnović, Anton; Missoni, Saša; Rootsi, Siiri; Rudan, Pavao

    2016-11-01

    The research objective of this study is to enlarge and deepen the Y chromosome research on the Croatian population and enable additional insights into the population diversity and historic events that shaped the current genetic landscape of Croatia and Southeastern Europe (SEE). A high-resolution phylogenetic and phylogeographic analysis of 66 biallelic (SNPs) and 17 microsatellite (STRs) markers of the Y chromosome was performed using 720 Croatian samples. The obtained results were placed in a wider European context by comparison with ∼4450 samples from a number of other European populations. A high diversity of haplogroups was observed in the overall Croatian sample, and all typical European Y chromosome haplogroups with corresponding clinal patterns were observed. Three distinct genetic signals were identifiable in the Croatian paternal gene pool - I2a1b-M423, R1a1a1b1a*-M558, and E1b1b1a1b1a-V13 haplogroups. The analyses of the dominant and autochthonous I2a1b-M423 lineage (>30%) suggest that SEE had a significant role in the Upper Paleolithic, the R1a1a1b1a*-M558 lineage (19%) represents a signal from present day Slavic populations of Central Europe in the Croatian population, and the phylogeography of the E1b1b1a1b1a-V13 clade (around 9%) implies cultural diffusion of agriculture into Europe via the Balkan Peninsula. Am. J. Hum. Biol., 2016. © 2016 Wiley Periodicals, Inc. Am. J. Hum. Biol. 28:837-845, 2016. © 2016Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  17. Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool.

    Science.gov (United States)

    Grabbe, Roman; Schmitz, Ruth A

    2003-04-01

    In Klebsiella pneumoniae, the flavoprotein, NifL regulates NifA mediated transcriptional activation of the N2-fixation (nif) genes in response to molecular O2 and ammonium. We investigated the influence of membrane-bound oxidoreductases on nif-regulation by biochemical analysis of purified NifL and by monitoring NifA-mediated expression of nifH'-'lacZ reporter fusions in different mutant backgrounds. NifL-bound FAD-cofactor was reduced by NADH only in the presence of a redox-mediator or inside-out vesicles derived from anaerobically grown K. pneumoniae cells, indicating that in vivo NifL is reduced by electrons derived from membrane-bound oxidoreductases of the anaerobic respiratory chain. This mechanism is further supported by three lines of evidence: First, K. pneumoniae strains carrying null mutations of fdnG or nuoCD showed significantly reduced nif-induction under derepressing conditions, indicating that NifL inhibition of NifA was not relieved in the absence of formate dehydrogenase-N or NADH:ubiquinone oxidoreductase. The same effect was observed in a heterologous Escherichia coli system carrying a ndh null allele (coding for NADH dehydrogenaseII). Second, studying nif-induction in K. pneumoniae revealed that during anaerobic growth in glycerol, under nitrogen-limitation, the presence of the terminal electron acceptor nitrate resulted in a significant decrease of nif-induction. The final line of evidence is that reduced quinone derivatives, dimethylnaphthoquinol and menadiol, are able to transfer electrons to the FAD-moiety of purified NifL. On the basis of these data, we postulate that under anaerobic and nitrogen-limited conditions, NifL inhibition of NifA activity is relieved by reduction of the FAD-cofactor by electrons derived from the reduced quinone pool, generated by anaerobic respiration, that favours membrane association of NifL. We further hypothesize that the quinol/quinone ratio is important for providing the signal to NifL.

  18. Swimming pool cleaner poisoning

    Science.gov (United States)

    Swimming pool cleaner poisoning occurs when someone swallows this type of cleaner, touches it, or breathes in ... The harmful substances in swimming pool cleaner are: Bromine ... copper Chlorine Soda ash Sodium bicarbonate Various mild acids

  19. Swimming pool granuloma

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/001357.htm Swimming pool granuloma To use the sharing features on this page, please enable JavaScript. A swimming pool granuloma is a long-term (chronic) skin ...

  20. Identification of Susceptibility Genes for Peritoneal, Ovarian, and Deep Infiltrating Endometriosis Using a Pooled Sample-Based Genome-Wide Association Study

    Directory of Open Access Journals (Sweden)

    Bruno Borghese

    2015-01-01

    Full Text Available Characterizing genetic contributions to endometriosis might help to shorten the time to diagnosis, especially in the most severe forms, but represents a challenge. Previous genome-wide association studies (GWAS made no distinction between peritoneal endometriosis (SUP, endometrioma (OMA, and deep infiltrating endometriosis (DIE. We therefore conducted a pooled sample-based GWAS and distinguished histologically confirmed endometriosis subtypes. We performed an initial discovery step on 10-individual pools (two pools per condition. After quality control filtering, a Monte-Carlo simulation was used to rank the significant SNPs according to the ratio of allele frequencies and the coefficient of variation. Then, a replication step of individual genotyping was conducted in an independent cohort of 259 cases and 288 controls. Our approach was very stringent but probably missed a lot of information due to the Monte-Carlo simulation, which likely explained why we did not replicate results from “classic” GWAS. Four variants (rs227849, rs4703908, rs2479037, and rs966674 were significantly associated with an increased risk of OMA. Rs4703908, located close to ZNF366, provided a higher risk of OMA (OR = 2.22; 95% CI: 1.26–3.92 and DIE, especially with bowel involvement (OR = 2.09; 95% CI: 1.12–3.91. ZNF366, involved in estrogen metabolism and progression of breast cancer, is a new biologically plausible candidate for endometriosis.

  1. Identification of susceptibility genes for peritoneal, ovarian, and deep infiltrating endometriosis using a pooled sample-based genome-wide association study.

    Science.gov (United States)

    Borghese, Bruno; Tost, Jörg; de Surville, Magalie; Busato, Florence; Letourneur, Frank; Mondon, Françoise; Vaiman, Daniel; Chapron, Charles

    2015-01-01

    Characterizing genetic contributions to endometriosis might help to shorten the time to diagnosis, especially in the most severe forms, but represents a challenge. Previous genome-wide association studies (GWAS) made no distinction between peritoneal endometriosis (SUP), endometrioma (OMA), and deep infiltrating endometriosis (DIE). We therefore conducted a pooled sample-based GWAS and distinguished histologically confirmed endometriosis subtypes. We performed an initial discovery step on 10-individual pools (two pools per condition). After quality control filtering, a Monte-Carlo simulation was used to rank the significant SNPs according to the ratio of allele frequencies and the coefficient of variation. Then, a replication step of individual genotyping was conducted in an independent cohort of 259 cases and 288 controls. Our approach was very stringent but probably missed a lot of information due to the Monte-Carlo simulation, which likely explained why we did not replicate results from "classic" GWAS. Four variants (rs227849, rs4703908, rs2479037, and rs966674) were significantly associated with an increased risk of OMA. Rs4703908, located close to ZNF366, provided a higher risk of OMA (OR = 2.22; 95% CI: 1.26-3.92) and DIE, especially with bowel involvement (OR = 2.09; 95% CI: 1.12-3.91). ZNF366, involved in estrogen metabolism and progression of breast cancer, is a new biologically plausible candidate for endometriosis.

  2. Solar swimming pool

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    This report examines the feasibility of using solar collectors to heat the water in a previously unheated outdoor swimming pool. The solar system is used in conjunction with a pool blanket, to conserve heat when the pool is not in use. Energy losses through evaporation can be reduced by as much as 70% by a pool blanket. A total of 130 m{sup 2} of highly durable black synthetic collectors were installed on a support structure at a 30{degree} angle from the horizontal, oriented to the south. Circulation of pool water though the collectors, which is controlled by a differential thermostat, was done with the existing pool pump. Before installation the pool temperature averaged 16{degree}C; after installation it ranged from 20{degree} to 26{degree}C. It was hard to distinguish how much pool heating was due to the solar system and how much heat was retained by the pool blanket. However, the pool season was extended by five weeks and attendance tripled. 2 figs.

  3. Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray ▿

    Science.gov (United States)

    Rato, Márcia G.; Nerlich, Andreas; Bergmann, René; Bexiga, Ricardo; Nunes, Sandro F.; Vilela, Cristina L.; Santos-Sanches, Ilda; Chhatwal, Gursharan S.

    2011-01-01

    A custom-designed microarray containing 220 virulence genes of Streptococcus pyogenes (group A Streptococcus [GAS]) was used to test group C Streptococcus dysgalactiae subsp. dysgalactiae (GCS) field strains causing bovine mastitis and group C or group G Streptococcus dysgalactiae subsp. equisimilis (GCS/GGS) isolates from human infections, with the latter being used for comparative purposes, for the presence of virulence genes. All bovine and all human isolates carried a fraction of the 220 genes (23% and 39%, respectively). The virulence genes encoding streptolysin S, glyceraldehyde-3-phosphate dehydrogenase, the plasminogen-binding M-like protein PAM, and the collagen-like protein SclB were detected in the majority of both bovine and human isolates (94 to 100%). Virulence factors, usually carried by human beta-hemolytic streptococcal pathogens, such as streptokinase, laminin-binding protein, and the C5a peptidase precursor, were detected in all human isolates but not in bovine isolates. Additionally, GAS bacteriophage-associated virulence genes encoding superantigens, DNase, and/or streptodornase were detected in bovine isolates (72%) but not in the human isolates. Determinants located in non-bacteriophage-related mobile elements, such as the gene encoding R28, were detected in all bovine and human isolates. Several virulence genes, including genes of bacteriophage origin, were shown to be expressed by reverse transcriptase PCR (RT-PCR). Phylogenetic analysis of superantigen gene sequences revealed a high level (>98%) of identity among genes of bovine GCS, of the horse pathogen Streptococcus equi subsp. equi, and of the human pathogen GAS. Our findings indicate that alpha-hemolytic bovine GCS, an important mastitis pathogen and considered to be a nonhuman pathogen, carries important virulence factors responsible for virulence and pathogenesis in humans. PMID:21525223

  4. Swimming-pool piles

    International Nuclear Information System (INIS)

    Trioulaire, M.

    1959-01-01

    In France two swimming-pool piles, Melusine and Triton, have just been set in operation. The swimming-pool pile is the ideal research tool for neutron fluxes of the order of 10 13 . This type of pile can be of immediate interest to many research centres, but its cost must be reduced and a break with tradition should be observed in its design. It would be an advantage: - to bury the swimming-pool; - to reject the experimental channel; - to concentrate the cooling circuit in the swimming-pool; - to carry out all manipulations in the water; - to double the core. (author) [fr

  5. Comparative De Novo Transcriptome Analysis of Fertilized Ovules in Xanthoceras sorbifolium Uncovered a Pool of Genes Expressed Specifically or Preferentially in the Selfed Ovule That Are Potentially Involved in Late-Acting Self-Incompatibility.

    Directory of Open Access Journals (Sweden)

    Qingyuan Zhou

    ovules of an LSI species. The availability of a pool of specifically or preferentially expressed genes from selfed ovules for X. sorbifolium will be a valuable resource for future genetic analyses of candidate genes involved in the LSI response.

  6. Spent fuel storage pool

    International Nuclear Information System (INIS)

    Murakami, Naoshi.

    1996-01-01

    Fences are disposed to a fuel exchange floor surrounding the upper surface of a fuel pool for preventing overflow of pool water. The fences comprise a plurality of flat boards arranged in parallel with each other in the longitudinal direction while being vertically inclined, and slits are disposed between the boards for looking down the pool. Further, the fences comprise wide boards and are constituted so as to be laid horizontally on the fuel exchange floor in a normal state and uprisen by means of the signals from an earthquake sensing device. Even if pool water is overflow from the fuel pool by the vibrations occurred upon earthquake and flown out to the floor of the fuel exchange floor, the overflow from the fuel exchange floor is prevented by the fences. An operator who monitors the fuel pool can observe the inside of the fuel pool through the slits formed to the fences during normal operation. The fences act as resistance against overflowing water upon occurrence of an earthquake thereby capable of reducing the overflowing amount of water due to the vibrations of pool water. The effect of preventing overflowing water can be enhanced. (N.H.)

  7. Collaborative Car Pooling System

    OpenAIRE

    João Ferreira; Paulo Trigo; Porfírio Filipe

    2009-01-01

    This paper describes the architecture for a collaborative Car Pooling System based on a credits mechanism to motivate the cooperation among users. Users can spend the accumulated credits on parking facilities. For this, we propose a business model to support the collaboration between a car pooling system and parking facilities. The Portuguese Lisbon-s Metropolitan area is used as application scenario.

  8. 13 CFR 120.611 - Pools backing Pool Certificates.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Pools backing Pool Certificates. 120.611 Section 120.611 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Secondary Market Certificates § 120.611 Pools backing Pool Certificates. (a) Pool characteristics. As set...

  9. Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum (Sorghum bicolor) treated with contrasting levels of nitrogen fertilizer

    NARCIS (Netherlands)

    Coelho, M.R.R.; Vos, de M.; Carneiro, N.P.; Marriel, I.E.; Paiva, E.; Seldin, L.

    2008-01-01

    The diversity of nitrogen-fixing bacteria was assessed in the rhizospheres of two cultivars of sorghum (IS 5322-C and IPA 1011) sown in Cerrado soil amended with two levels of nitrogen fertilizer (12 and 120 kg ha(-1)). The nifH gene was amplified directly from DNA extracted from the rhizospheres,

  10. Genetic variation in the immunosuppression pathway genes and breast cancer: a pooled analysis of 42,510 cases and 40,577 controls from the Breast

    OpenAIRE

    Lei, Jieping; Rudolph, Anja; Moysich, Kirsten B; Behrens, Sabine; Goode, Ellen L; Bolla, Manjeet K; Dennis, Joe; Dunning, Alison Margaret; Easton, Douglas Frederick; Wang, Qin; Benitez, Javier; Hopper, John L; Southey, Melissa C; Schmidt, Marjanka K; Broeks, Annegien

    2015-01-01

    Immunosuppression plays a pivotal role in assisting tumors to evade immune destruction and promoting tumor development. We hypothesized that genetic variation in the immunosuppression pathway genes may be implicated in breast cancer tumorigenesis. We included 42,510 female breast cancer cases and 40,577 controls of European ancestry from 37 studies in the Breast Cancer Association Consortium (2015) with available genotype data for 3595 single nucleotide polymorphisms (SNPs) in 133 candidate g...

  11. [Dose-Response Dependences for Frequency of RET/PTC Gene Rearrangements in Papillary Thyroid Carcinoma after Irradiation. Simple Pooling Analysis of Molecular Epidemiological Data].

    Science.gov (United States)

    Koterov, A N; Ushenkova, L N; Biryukov, A P

    2016-01-01

    On the basis of all possible publications on the theme included in the previously formed base of sources on molecular epidemiology of RET/PTC rearrangements in thyroid papillary carcinoma a pooled analysis ("simple pooling data") on determination of the dose-effect dependences for RET/PTC frequency in radiogenic carcinomas of various irradiated groups was performed. (They are groups subjected to radiotherapeutic exposure, residents near the Chernobyl nuclear power plant (CNPP) and victims of nuclear bombing). The tendency to Pearson linear correlation (r = 0.746; p = 0.148) between the frequency of RET/PTC and the estimated dose on thyroid in the regions affected by the CNPP accident was revealed. But this tendency was recognized to be random owing to abnormally low values of the indicator for the most contaminated Gomel region. The method tentatively called "case-control" showed reliable differences in thyroid dose values for carcinomas with RET/PTC and without those. The versatility of changes was found: the lack of RET/PTC for radiotherapeutic impacts was associated with higher doses, whereas in case of the CNPP accident and for nuclear bombing victims it was the opposite. Probably, in the first case the "cellular cleaning" phenomenon after exposure to very high doses took place. Search of direct Pearson correlations between average/median thyroid doses on groups and RET/PTC frequency in carcinomas of these groups showed a high reliability for the dose-effect dependences- at the continuous dose scale (for RET/PTC in total and RET/PTC1 respectively: r = 0.830; p = 0.002 and r = 0.906; p = 0.0003); while there was no significant correlation received for RET/PTC3. When using the weighting least square regression analysis (proceeding from the number of carcinomas in samples), the specified regularities remained. Attempts to influence the strength of correlation by exception ofthe data of all the samples connected with the accident on the CNPP did not significantly

  12. Metschnikowia Species Share a Pool of Diverse rRNA Genes Differing in Regions That Determine Hairpin-Loop Structures and Evolve by Reticulation.

    Directory of Open Access Journals (Sweden)

    Matthias Sipiczki

    Full Text Available Modern taxonomy of yeasts is mainly based on phylogenetic analysis of conserved DNA and protein sequences. By far the most frequently used sequences are those of the repeats of the chromosomal rDNA array. It is generally accepted that the rDNA repeats of a genome have identical sequences due to the phenomenon of sequence homogenisation and can thus be used for identification and barcoding of species. Here we show that the rDNA arrays of the type strains of Metschnikowia andauensis and M. fructicola are not homogenised. Both have arrays consisting of diverse repeats that differ from each other in the D1/D2 domains by up to 18 and 25 substitutions. The variable sites are concentrated in two regions that correspond to back-folding stretches of hairpin loops in the predicted secondary structure of the RNA molecules. The substitutions do not alter significantly the overall hairpin-loop structure due to wobble base pairing at sites of C-T transitions and compensatory mutations in the complementary strand of the hairpin stem. The phylogenetic and network analyses of the cloned sequences revealed that the repeats had not evolved in a vertical tree-like way but reticulation might have shaped the rDNA arrays of both strains. The neighbour-net analysis of all cloned sequences of the type strains and the database sequences of different strains further showed that these species share a continuous pool of diverse repeats that appear to evolve by reticulate evolution.

  13. Swimming Pool Safety

    Science.gov (United States)

    ... Spread the Word Shop AAP Find a Pediatrician Safety & Prevention Immunizations All Around At Home At Play ... Español Text Size Email Print Share Swimming Pool Safety Page Content ​What is the best way to ...

  14. Vitamin D Pooling Project

    Science.gov (United States)

    The Vitamin D Pooling Project of Rarer Cancers brought together investigators from 10 cohorts to conduct a large prospective epidemiologic study of the association between vitamin D status and seven rarer cancers.

  15. Swimming pool special; Zwembadspecial

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-05-15

    This issue includes a few articles and messages on the use of heat pump systems in swimming pools. [Dutch] Dit nummer bevat onder meer een paar artikelen over het gebruik van warmtepompsystemen in zwembaden.

  16. Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum (Sorghum bicolor) treated with contrasting levels of nitrogen fertilizer.

    Science.gov (United States)

    Coelho, Marcia Reed Rodrigues; de Vos, Marjon; Carneiro, Newton Portilho; Marriel, Ivanildo Evódio; Paiva, Edilson; Seldin, Lucy

    2008-02-01

    The diversity of nitrogen-fixing bacteria was assessed in the rhizospheres of two cultivars of sorghum (IS 5322-C and IPA 1011) sown in Cerrado soil amended with two levels of nitrogen fertilizer (12 and 120 kg ha(-1)). The nifH gene was amplified directly from DNA extracted from the rhizospheres, and the PCR products cloned and sequenced. Four clone libraries were generated from the nifH fragments and 245 sequences were obtained. Most of the clones (57%) were closely related to nifH genes of uncultured bacteria. NifH clones affiliated with Azohydromonas spp., Ideonella sp., Rhizobium etli and Bradyrhizobium sp. were found in all libraries. Sequences affiliated with Delftia tsuruhatensis were found in the rhizosphere of both cultivars sown with high levels of nitrogen, while clones affiliated with Methylocystis sp. were detected only in plants sown under low levels of nitrogen. Moreover, clones affiliated with Paenibacillus durus could be found in libraries from the cultivar IS 5322-C sown either in high or low amounts of fertilizer. This study showed that the amount of nitrogen used for fertilization is the overriding determinative factor that influenced the nitrogen-fixing community structures in sorghum rhizospheres cultivated in Cerrado soil.

  17. Pool water cleaning facility

    Energy Technology Data Exchange (ETDEWEB)

    Yoshikawa, Kazuhiro; Kinoshita, Shoichiro [Hitachi Ltd., Tokyo (Japan); Asano, Takashi

    1998-05-29

    Only one system comprising a suppression poor water cleaning system (SPCU) and a filtration desalting tower (F/D) is connected for a plurality of nuclear power plants. Pipelines/valves for connecting the one system of the SPCU pump, the F/D and the plurality of nuclear power plants are disposed, and the system is used in common with the plurality of nuclear power plants. Pipelines/valves for connecting a pipeline for passing SP water to the commonly used SPCU pump and a skimmer surge tank are disposed, and fuel pool water is cooled and cleaned by the commonly used SPCU pump and the commonly used F/D. The number of SPCU pumps and the F/D facilities can be reduced, and a fuel pool water cooling operation mode and a fuel pool water cleaning operation mode which were conducted by an FPC pump so far are conducted by the SPCU pump. (N.H.)

  18. Liquid sodium pool fires

    Energy Technology Data Exchange (ETDEWEB)

    Casselman, C [DSN/SESTR, Centre de Cadarache, Saint-Paul-lez-Durance (France)

    1979-03-01

    Experimental sodium pool combustion results have led to a definition of the combustion kinetics, and have revealed the hazards of sodium-concrete contact reactions and the possible ignition of organic matter (paint) by hydration of sodium peroxide aerosols. Analysis of these test results shows that the controlling mechanism is sodium evaporation diffusion. (author)

  19. Income pooling within families

    DEFF Research Database (Denmark)

    Bonke, Jens; Uldall-Poulsen, Hans

    This paper analyses the phenomenon of income-pooling by applying the Danish household expenditure survey, merged with authoritative register information. Responses to additional questions on income sharing among 1696 couples also allows us to analyses whether the intra-household distribution...

  20. Liquid sodium pool fires

    International Nuclear Information System (INIS)

    Casselman, C.

    1979-01-01

    Experimental sodium pool combustion results have led to a definition of the combustion kinetics, and have revealed the hazards of sodium-concrete contact reactions and the possible ignition of organic matter (paint) by hydration of sodium peroxide aerosols. Analysis of these test results shows that the controlling mechanism is sodium evaporation diffusion. (author)

  1. Pool gateway seal

    International Nuclear Information System (INIS)

    Starr, J.A.; Steinert, L.A.

    1983-01-01

    A device for sealing a gateway between interconnectable pools in a nuclear facility comprising a frame supporting a liquid impermeable sheet positioned in a u-shaped gateway between the pools. An inflatable tube carried in a channel in the periphery of the frame and adjoining the gateway provides a seal therebetween when inflated. A restraining arrangement on the bottom edge of the frame is releasably engagable with an adjacent portion of the gateway to restrict the movement of the frame in the u-shaped gateway upon inflation of the tube, thereby enhancing the seal. The impermeable sheet is formed of an elastomer and thus is conformable to a liquid permeable supportive wall upon application of liquid pressure to the side of the sheet opposite the wall

  2. Backfitting swimming pool reactors

    International Nuclear Information System (INIS)

    Roebert, G.A.

    1978-01-01

    Calculations based on measurements in a critical assembly, and experiments to disclose fuel element surface temperatures in case of accidents like stopping of primary coolant flow during full power operation, have shown that the power of the swimming pool type research reactor FRG-2 (15 MW, operating since 1967) might be raised to 21 MW within the present rules of science and technology, without major alterations of the pool buildings and the cooling systems. A backfitting program is carried through to adjust the reactor control systems of FRG-2 and FRG-1 (5 MW, housed in the same reactor hall) to the present safety rules and recommendations, to ensure FRG-2 operation at 21 MW for the next decade. (author)

  3. Fuel assembly storage pool

    International Nuclear Information System (INIS)

    Hiranuma, Hiroshi.

    1976-01-01

    Object: To remove limitation of the number of storage of fuel assemblies to increase the number of storage thereof so as to relatively reduce the water depth required for shielding radioactive rays. Structure: Fuel assembly storage rack containers for receiving a plurality of spent fuel assembly racks are stacked in multi-layer fashion within a storage pool filled with water for shielding radioactive rays and removing heat. (Furukawa, Y.)

  4. CERN Electronics Pool presentations

    CERN Multimedia

    2011-01-01

    The CERN Electronics Pool has organised a series of presentations in collaboration with oscilloscope manufacturers. The last one will take place according to the schedule below.   Time will be available at the end of the presentation to discuss your personal needs. The Agilent presentation had to be postponed and will be organised later. -     Lecroy: Thursday, 24 November 2011, in 530-R-030, 14:00 to 16:30.

  5. The flexible gene pool of Propionibacterium acnes

    DEFF Research Database (Denmark)

    Brüggemann, Holger; Lomholt, Hans B; Kilian, Mogens

    2012-01-01

    Propionibacterium acnes is a Gram-positive bacterium that is intimately associated with humans. The nature and consequences of this symbiosis are poorly understood; it might comprise both mutualistic and parasitic properties. Recent advances in distinguishing phylotypes of P. acnes have revealed ...

  6. Project charts Estonia's gene pool / Tricia Cornell

    Index Scriptorium Estoniae

    Cornell, Tricia

    1999-01-01

    Sihtasutuse Geenikeskus rajajad tuli välja ideega luua Eesti Geenivaramu, mis koosneks kahest andmekogust - inimese tervisliku seisundi kirjeldusest (fenotüüp) ja tema geenikombinatsiooni kirjeldusest (genotüüp)

  7. Swimming Pools and Molluscum Contagiosum

    Science.gov (United States)

    ... Travelers’ Health: Smallpox & Other Orthopoxvirus-Associated Infections Poxvirus Swimming Pools Recommend on Facebook Tweet Share Compartir The ... often ask if molluscum virus can spread in swimming pools. There is also concern that it can ...

  8. Pool-type reactor

    International Nuclear Information System (INIS)

    Hopkins, S.R.

    1977-01-01

    This invention relates to a pool nuclear reactor fitted with a perfected system to raise the buckets into a vertical position at the bottom of a channel. This reactor has an inclined channel to guide a bucket containing a fuel assembly to introduce it into the reactor jacket or extract it therefrom and a damper at the bottom of the channel to stop the drop of the bucket. An upright vertically movable rod has a horizontally articulated arm with a hook. This can pivot to touch a radial lug on the bucket and pivot the bucket around its base in a vertical position, when the rod moves up [fr

  9. Estabilidade de produção de híbridos simples e duplos de milho oriundos de um mesmo conjunto gênico Yield stability in single and double crosses of maize originated from the same gene pool

    Directory of Open Access Journals (Sweden)

    Juarez Campolina Machado

    2008-01-01

    Full Text Available O presente trabalho foi realizado com o objetivo de comparar a estabilidade de híbridos simples e híbridos duplos de milho oriundos de um mesmo conjunto gênico. Foram avaliados 55 tratamentos, sendo dez híbridos simples comerciais, utilizados como parentais e 45 híbridos duplos resultantes de um dialelo completo. As sementes dos híbridos duplos foram obtidas na área experimental do Departamento de Biologia da Universidade Federal de Lavras (DBI/UFLA. Os experimentos foram desenvolvidos em 15 ambientes, no ano agrícola de 2005/2006, em propriedades de agricultores e estações experimentais. O delineamento experimental utilizado foi o de blocos casualizados com três repetições e o caráter avaliado foi a produtividade de espigas despalhadas (kg ha-1, corrigida para 13% de umidade. Obteve-se a contribuição de cada híbrido para a interação genótipos x ambientes e o desvio em relação ao desempenho máximo em cada ambiente utilizando a estatística não-paramétrica por meio da soma de postos. Os híbridos duplos foram, em média, mais estáveis, contudo, identificaram-se híbridos simples tão estáveis quanto os duplos.The objective of the present work was to study the adaptability and stability of single and double-crosses of maize originated from the same gene pool. Ten commercial single-crosses and all possible double-crosses, obtained from a complete diallel, were evaluated. Seeds of the double-crosses were obtained in an experimental area of the Biology Department at Universidade Federal de Lavras (DBI/UFLA. The experiments were conducted in 15 environments in the 2005/06 growing season, on farms and in experimental stations. The cultural practices were the ones normally used by farmers or experimental stations for maize. The entries were evaluated in randomized complete blocks design with three replications per environment. The trait under study was husked ears yield (kg ha-1, corrected to 13% of moisture content

  10. Model of large pool fires

    Energy Technology Data Exchange (ETDEWEB)

    Fay, J.A. [Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States)]. E-mail: jfay@mit.edu

    2006-08-21

    A two zone entrainment model of pool fires is proposed to depict the fluid flow and flame properties of the fire. Consisting of combustion and plume zones, it provides a consistent scheme for developing non-dimensional scaling parameters for correlating and extrapolating pool fire visible flame length, flame tilt, surface emissive power, and fuel evaporation rate. The model is extended to include grey gas thermal radiation from soot particles in the flame zone, accounting for emission and absorption in both optically thin and thick regions. A model of convective heat transfer from the combustion zone to the liquid fuel pool, and from a water substrate to cryogenic fuel pools spreading on water, provides evaporation rates for both adiabatic and non-adiabatic fires. The model is tested against field measurements of large scale pool fires, principally of LNG, and is generally in agreement with experimental values of all variables.

  11. Model of large pool fires

    International Nuclear Information System (INIS)

    Fay, J.A.

    2006-01-01

    A two zone entrainment model of pool fires is proposed to depict the fluid flow and flame properties of the fire. Consisting of combustion and plume zones, it provides a consistent scheme for developing non-dimensional scaling parameters for correlating and extrapolating pool fire visible flame length, flame tilt, surface emissive power, and fuel evaporation rate. The model is extended to include grey gas thermal radiation from soot particles in the flame zone, accounting for emission and absorption in both optically thin and thick regions. A model of convective heat transfer from the combustion zone to the liquid fuel pool, and from a water substrate to cryogenic fuel pools spreading on water, provides evaporation rates for both adiabatic and non-adiabatic fires. The model is tested against field measurements of large scale pool fires, principally of LNG, and is generally in agreement with experimental values of all variables

  12. Design of hydrotherapy exercise pools.

    Science.gov (United States)

    Edlich, R F; Abidin, M R; Becker, D G; Pavlovich, L J; Dang, M T

    1988-01-01

    Several hydrotherapy pools have been designed specifically for a variety of aquatic exercise. Aqua-Ark positions the exerciser in the center of the pool for deep-water exercise. Aqua-Trex is a shallow underwater treadmill system for water walking or jogging. Swim-Ex generates an adjustable laminar flow that permits swimming without turning. Musculoskeletal conditioning can be accomplished in the above-ground Arjo shallow-water exercise pool. A hydrotherapy pool also can be custom designed for musculoskeletal conditioning in its shallow part and cardiovascular conditioning in a deeper portion of the pool. Regardless of the type of exercise, there is general agreement that the specific exercise conducted in water requires significantly more energy expenditure than when the same exercise is performed on land.

  13. Gene

    Data.gov (United States)

    U.S. Department of Health & Human Services — Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes,...

  14. ENERGY STAR Certified Pool Pumps

    Data.gov (United States)

    U.S. Environmental Protection Agency — Certified models meet all ENERGY STAR requirements as listed in the Version 1.1 ENERGY STAR Program Requirements for Pool Pumps that are effective as of February 15,...

  15. Pooling and correlated neural activity

    Directory of Open Access Journals (Sweden)

    Robert Rosenbaum

    2010-04-01

    Full Text Available Correlations between spike trains can strongly modulate neuronal activity and affect the ability of neurons to encode information. Neurons integrate inputs from thousands of afferents. Similarly, a number of experimental techniques are designed to record pooled cell activity. We review and generalize a number of previous results that show how correlations between cells in a population can be amplified and distorted in signals that reflect their collective activity. The structure of the underlying neuronal response can significantly impact correlations between such pooled signals. Therefore care needs to be taken when interpreting pooled recordings, or modeling networks of cells that receive inputs from large presynaptic populations. We also show that the frequently observed runaway synchrony in feedforward chains is primarily due to the pooling of correlated inputs.

  16. Grundfoss: Chlorination of Swimming Pools

    DEFF Research Database (Denmark)

    Hjorth, Poul G.; Hogan, John; Andreassen, Viggo

    1998-01-01

    Grundfos asked for a model, describing the problem of mixing chemicals, being dosed into water systems, to be developed. The application of the model should be dedicated to dosing aqueous solution of chlorine into swimming pools.......Grundfos asked for a model, describing the problem of mixing chemicals, being dosed into water systems, to be developed. The application of the model should be dedicated to dosing aqueous solution of chlorine into swimming pools....

  17. Sustainability of common pool resources

    OpenAIRE

    Timilsina, Raja Rajendra; Kotani, Koji; Kamijo, Yoshio

    2017-01-01

    Sustainability has become a key issue in managing natural resources together with growing concerns for capitalism, environmental and resource problems. We hypothesize that the ongoing modernization of competitive societies, which we refer to as "capitalism," affects human nature for utilizing common pool resources, thus compromising sustainability. To test this hypothesis, we design and implement a set of dynamic common pool resource games and experiments in the following two types of Nepales...

  18. Seismic analysis of large pools

    Energy Technology Data Exchange (ETDEWEB)

    Dong, R.G.; Tokarz, F.J.

    1976-11-17

    Large pools for storing spent, nuclear fuel elements are being proposed to augment present storage capacity. To preserve the ability to isolate portions of these pools, a modularization requirement appears desirable. The purpose of this project was to investigate the effects of modularization on earthquake resistance and to assess the adequacy of current design methods for seismic loads. After determining probable representative pool geometries, three rectangular pool configurations, all 240 x 16 ft and 40 ft deep, were examined. One was unmodularized; two were modularized into 80 x 40 ft cells in one case and 80 x 80 ft cells in the other. Both embedded and above-ground installations for a hard site and embedded installations for an intermediate hard site were studied. It was found that modularization was unfavorable in terms of reducing the total structural load attributable to dynamic effects, principally because one or more cells could be left unfilled. The walls of unfilled cells would be subjected to significantly higher loads than the walls of a filled, unmodularized pool. Generally, embedded installations were preferable to above-ground installations, and the hard site was superior to the intermediate hard site. It was determined that Housner's theory was adequate for calculating hydrodynamic effects on spent fuel storage pools. Current design methods for seismic loads were found to be satisfactory when results from these methods were compared with those from LUSH analyses. As a design method for dynamic soil pressure, we found the Mononobe-Okabe theory, coupled with correction factors as suggested by Seed, to be acceptable. The factors we recommend for spent fuel storage pools are tabulated.

  19. Seismic analysis of large pools

    International Nuclear Information System (INIS)

    Dong, R.G.; Tokarz, F.J.

    1976-01-01

    Large pools for storing spent, nuclear fuel elements are being proposed to augment present storage capacity. To preserve the ability to isolate portions of these pools, a modularization requirement appears desirable. The purpose of this project was to investigate the effects of modularization on earthquake resistance and to assess the adequacy of current design methods for seismic loads. After determining probable representative pool geometries, three rectangular pool configurations, all 240 x 16 ft and 40 ft deep, were examined. One was unmodularized; two were modularized into 80 x 40 ft cells in one case and 80 x 80 ft cells in the other. Both embedded and above-ground installations for a hard site and embedded installations for an intermediate hard site were studied. It was found that modularization was unfavorable in terms of reducing the total structural load attributable to dynamic effects, principally because one or more cells could be left unfilled. The walls of unfilled cells would be subjected to significantly higher loads than the walls of a filled, unmodularized pool. Generally, embedded installations were preferable to above-ground installations, and the hard site was superior to the intermediate hard site. It was determined that Housner's theory was adequate for calculating hydrodynamic effects on spent fuel storage pools. Current design methods for seismic loads were found to be satisfactory when results from these methods were compared with those from LUSH analyses. As a design method for dynamic soil pressure, we found the Mononobe-Okabe theory, coupled with correction factors as suggested by Seed, to be acceptable. The factors we recommend for spent fuel storage pools are tabulated

  20. 21 CFR 1250.89 - Swimming pools.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Swimming pools. 1250.89 Section 1250.89 Food and... SANITATION Sanitation Facilities and Conditions on Vessels § 1250.89 Swimming pools. (a) Fill and draw swimming pools shall not be installed or used. (b) Swimming pools of the recirculation type shall be...

  1. Patent pools: Intellectual property rights and competition.

    NARCIS (Netherlands)

    Rodriguez, V.F.

    2010-01-01

    Patent pools do not correct all problems associated with patent thickets. In this respect, patent pools might not stop the outsider problem from striking pools. Moreover, patent pools can be expensive to negotiate, can exclude patent holders with smaller numbers of patents or enable a group of major

  2. Efficient pooling designs for library screening

    OpenAIRE

    Bruno, William J.; Knill, Emanuel; Balding, David J.; Bruce, D. C.; Doggett, N. A.; Sawhill, W. W.; Stallings, R. L.; Whittaker, Craig C.; Torney, David C.

    1994-01-01

    We describe efficient methods for screening clone libraries, based on pooling schemes which we call ``random $k$-sets designs''. In these designs, the pools in which any clone occurs are equally likely to be any possible selection of $k$ from the $v$ pools. The values of $k$ and $v$ can be chosen to optimize desirable properties. Random $k$-sets designs have substantial advantages over alternative pooling schemes: they are efficient, flexible, easy to specify, require fewer pools, and have er...

  3. EP BICYCLE POOL - VIGNETTES 2002

    CERN Multimedia

    EP-SMI Help Desk

    2002-01-01

    The vignettes (insurance certificates) for 2002 become obligatory from 1 June. If you have a bicycle from the EP Pool, please bring it to the EP-SMI Help Desk (Building 124) on any working day up to 31 May between 8h.30 - 12h.00 or 13h.30 - 17h.30. EP-SMI Help Desk

  4. Genetic variation in the immunosuppression pathway genes and breast cancer susceptibility: a pooled analysis of 42,510 cases and 40,577 controls from the Breast Cancer Association Consortium.

    Science.gov (United States)

    Lei, Jieping; Rudolph, Anja; Moysich, Kirsten B; Behrens, Sabine; Goode, Ellen L; Bolla, Manjeet K; Dennis, Joe; Dunning, Alison M; Easton, Douglas F; Wang, Qin; Benitez, Javier; Hopper, John L; Southey, Melissa C; Schmidt, Marjanka K; Broeks, Annegien; Fasching, Peter A; Haeberle, Lothar; Peto, Julian; Dos-Santos-Silva, Isabel; Sawyer, Elinor J; Tomlinson, Ian; Burwinkel, Barbara; Marmé, Frederik; Guénel, Pascal; Truong, Thérèse; Bojesen, Stig E; Flyger, Henrik; Nielsen, Sune F; Nordestgaard, Børge G; González-Neira, Anna; Menéndez, Primitiva; Anton-Culver, Hoda; Neuhausen, Susan L; Brenner, Hermann; Arndt, Volker; Meindl, Alfons; Schmutzler, Rita K; Brauch, Hiltrud; Hamann, Ute; Nevanlinna, Heli; Fagerholm, Rainer; Dörk, Thilo; Bogdanova, Natalia V; Mannermaa, Arto; Hartikainen, Jaana M; Van Dijck, Laurien; Smeets, Ann; Flesch-Janys, Dieter; Eilber, Ursula; Radice, Paolo; Peterlongo, Paolo; Couch, Fergus J; Hallberg, Emily; Giles, Graham G; Milne, Roger L; Haiman, Christopher A; Schumacher, Fredrick; Simard, Jacques; Goldberg, Mark S; Kristensen, Vessela; Borresen-Dale, Anne-Lise; Zheng, Wei; Beeghly-Fadiel, Alicia; Winqvist, Robert; Grip, Mervi; Andrulis, Irene L; Glendon, Gord; García-Closas, Montserrat; Figueroa, Jonine; Czene, Kamila; Brand, Judith S; Darabi, Hatef; Eriksson, Mikael; Hall, Per; Li, Jingmei; Cox, Angela; Cross, Simon S; Pharoah, Paul D P; Shah, Mitul; Kabisch, Maria; Torres, Diana; Jakubowska, Anna; Lubinski, Jan; Ademuyiwa, Foluso; Ambrosone, Christine B; Swerdlow, Anthony; Jones, Michael; Chang-Claude, Jenny

    2016-01-01

    Immunosuppression plays a pivotal role in assisting tumors to evade immune destruction and promoting tumor development. We hypothesized that genetic variation in the immunosuppression pathway genes may be implicated in breast cancer tumorigenesis. We included 42,510 female breast cancer cases and 40,577 controls of European ancestry from 37 studies in the Breast Cancer Association Consortium (2015) with available genotype data for 3595 single nucleotide polymorphisms (SNPs) in 133 candidate genes. Associations between genotyped SNPs and overall breast cancer risk, and secondarily according to estrogen receptor (ER) status, were assessed using multiple logistic regression models. Gene-level associations were assessed based on principal component analysis. Gene expression analyses were conducted using RNA sequencing level 3 data from The Cancer Genome Atlas for 989 breast tumor samples and 113 matched normal tissue samples. SNP rs1905339 (A>G) in the STAT3 region was associated with an increased breast cancer risk (per allele odds ratio 1.05, 95 % confidence interval 1.03-1.08; p value = 1.4 × 10(-6)). The association did not differ significantly by ER status. On the gene level, in addition to TGFBR2 and CCND1, IL5 and GM-CSF showed the strongest associations with overall breast cancer risk (p value = 1.0 × 10(-3) and 7.0 × 10(-3), respectively). Furthermore, STAT3 and IL5 but not GM-CSF were differentially expressed between breast tumor tissue and normal tissue (p value = 2.5 × 10(-3), 4.5 × 10(-4) and 0.63, respectively). Our data provide evidence that the immunosuppression pathway genes STAT3, IL5, and GM-CSF may be novel susceptibility loci for breast cancer in women of European ancestry.

  5. "Ripples" in an Aluminum Pool?

    Science.gov (United States)

    Rohr, James; Wang, Si-Yin; Nesterenko, Vitali F.

    2018-05-01

    Our motivation for this article is for students to realize that opportunities for discovery are all around them. Discoveries that can still puzzle present day researchers. Here we explore an observation by a middle school student concerning the production of what appears to be water-like "ripples" produced in aluminum foil when placed between two colliding spheres. We both applaud and explore the student's reasoning that the ripples were formed in a melted aluminum pool.

  6. Sustainability of common pool resources.

    Science.gov (United States)

    Timilsina, Raja Rajendra; Kotani, Koji; Kamijo, Yoshio

    2017-01-01

    Sustainability has become a key issue in managing natural resources together with growing concerns for capitalism, environmental and resource problems. We hypothesize that the ongoing modernization of competitive societies, which we refer to as "capitalism," affects human nature for utilizing common pool resources, thus compromising sustainability. To test this hypothesis, we design and implement a set of dynamic common pool resource games and experiments in the following two types of Nepalese areas: (i) rural (non-capitalistic) and (ii) urban (capitalistic) areas. We find that a proportion of prosocial individuals in urban areas is lower than that in rural areas, and urban residents deplete resources more quickly than rural residents. The composition of proself and prosocial individuals in a group and the degree of capitalism are crucial in that an increase in prosocial members in a group and the rural dummy positively affect resource sustainability by 65% and 63%, respectively. Overall, this paper shows that when societies move toward more capitalistic environments, the sustainability of common pool resources tends to decrease with the changes in individual preferences, social norms, customs and views to others through human interactions. This result implies that individuals may be losing their coordination abilities for social dilemmas of resource sustainability in capitalistic societies.

  7. Flashing oscillation in pool water

    International Nuclear Information System (INIS)

    Takamasa, Tomoji; Kondo, Koichi; Hazuku, Tatsuya

    1996-01-01

    This paper presents an experimental study of high-pressure saturated water discharging into the pool water. The purpose of the experiment is to clarify the phenomena that occur in blow-down of high-pressure saturated water from the pressure vessel into the water-filled containment in the case of a wall-crack accident or a LOCA in an advanced reactor. The results revealed that a flashing oscillation (FO) occurs when high-pressure saturated water discharges into the pool water, under specified experimental settings. The range of the flashing oscillates between a point very close to and some distance from the vent hole. The pressures in the vent tube and pool water vary according to the flashing oscillation. The pressure oscillation and frequency of flashing position might be caused by the balancing action between the supply of saturated water, flashing at the control volume and its condensation on the steam-water interface. A linear analysis was conducted using a spherical flashing bubble model. The period of the flashing oscillation in the experiments can be explained by theoretical analysis

  8. Pressure supression pool thermal mixing

    International Nuclear Information System (INIS)

    Cook, D.H.

    1984-10-01

    A model is developed and verified to describe the thermal mixing that occurs in the pressure suppression pool (PSP) of a commercial BWR. The model is designed specifically for a Mark-I containment and is intended for use in severe accident sequence analyses. The model developed in this work produces space and time dependent temperature results throughout the PSP and is useful for evaluating the bulk PSP thermal mixing, the condensation effectiveness of the PSP, and the long-term containment integrity. The model is designed to accommodate single or multiple discharging T-quenchers, a PSP circumferential circulation induced by the residual heat removal system discharge, and the thermal stratification of the pool that occurs immediately after the relief valves close. The PSP thermal mixing is verified by comparing the model-predicted temperatures to experimental temperatures that were measured in an operating BWR suppression pool. The model is then used to investigate several PSP thermal mixing problems that include the time to saturate at full relief valve flow, the temperature response to a typical stuck open relief valve scenario, and the effect of operator rotation of the relief valve discharge point

  9. Pressure suppression pool thermal mixing

    International Nuclear Information System (INIS)

    Cook, D.H.

    1984-01-01

    A model is developed and verified to describe the thermal mixing that occurs in the pressure suppression pool (PSP) of a commercial BWR. The model is designed specifically for a Mark-I containment and is intended for use in severe accident sequence analyses. The model produces space and time dependent temperature results throughout the PSP and is useful for evaluating the bulk PSP thermal mixing, the condensation effectiveness of the PSP, and the long-term containment integrity. The model is designed to accommodate single or multiple discharging T-quenchers, a PSP circumferential circulation induced by the residual heat removal system discharge, and the thermal stratification of the pool that occurs immediately after the relief valves close. The PSP thermal mixing model is verified by comparing the model predicted temperatures to experimental temperatures that were measured in an operating BWR suppression pool. The model is then used to investigate several PSP thermal mixing problems that include the time to saturate at full relief valve flow, the temperature response to a typical stuck open relief valve scenario, and the effect of operator rotation of the relief valve discharge point

  10. UPDG: Utilities package for data analysis of Pooled DNA GWAS

    Directory of Open Access Journals (Sweden)

    Ho Daniel WH

    2012-01-01

    Full Text Available Abstract Background Despite being a well-established strategy for cost reduction in disease gene mapping, pooled DNA association study is much less popular than the individual DNA approach. This situation is especially true for pooled DNA genomewide association study (GWAS, for which very few computer resources have been developed for its data analysis. This motivates the development of UPDG (Utilities package for data analysis of Pooled DNA GWAS. Results UPDG represents a generalized framework for data analysis of pooled DNA GWAS with the integration of Unix/Linux shell operations, Perl programs and R scripts. With the input of raw intensity data from GWAS, UPDG performs the following tasks in a stepwise manner: raw data manipulation, correction for allelic preferential amplification, normalization, nested analysis of variance for genetic association testing, and summarization of analysis results. Detailed instructions, procedures and commands are provided in the comprehensive user manual describing the whole process from preliminary preparation of software installation to final outcome acquisition. An example dataset (input files and sample output files is also included in the package so that users can easily familiarize themselves with the data file formats, working procedures and expected output. Therefore, UPDG is especially useful for users with some computer knowledge, but without a sophisticated programming background. Conclusions UPDG provides a free, simple and platform-independent one-stop service to scientists working on pooled DNA GWAS data analysis, but with less advanced programming knowledge. It is our vision and mission to reduce the hindrance for performing data analysis of pooled DNA GWAS through our contribution of UPDG. More importantly, we hope to promote the popularity of pooled DNA GWAS, which is a very useful research strategy.

  11. CDC Study Finds Fecal Contamination in Pools

    Science.gov (United States)

    ... Communication (404) 639-3286 CDC study finds fecal contamination in pools A study of public pools done ... The E. coli is a marker for fecal contamination. Finding a high percentage of E. coli-positive ...

  12. 1968 Listing of Swimming Pool Equipment.

    Science.gov (United States)

    National Sanitation Foundation, Ann Arbor, MI. Testing Lab.

    An up-to-date listing of swimming pool equipment including--(1) companies authorized to display the National Sanitation Foundation seal of approval, (2) equipment listed as meeting NSF swimming pool equipment standards relating to diatomite type filters, (3) equipment listed as meeting NSF swimming pool equipment standard relating to sand type…

  13. Finding Biomarker Signatures in Pooled Sample Designs: A Simulation Framework for Methodological Comparisons

    Directory of Open Access Journals (Sweden)

    Anna Telaar

    2010-01-01

    Full Text Available Detection of discriminating patterns in gene expression data can be accomplished by using various methods of statistical learning. It has been proposed that sample pooling in this context would have negative effects; however, pooling cannot always be avoided. We propose a simulation framework to explicitly investigate the parameters of patterns, experimental design, noise, and choice of method in order to find out which effects on classification performance are to be expected. We use a two-group classification task and simulated gene expression data with independent differentially expressed genes as well as bivariate linear patterns and the combination of both. Our results show a clear increase of prediction error with pool size. For pooled training sets powered partial least squares discriminant analysis outperforms discriminance analysis, random forests, and support vector machines with linear or radial kernel for two of three simulated scenarios. The proposed simulation approach can be implemented to systematically investigate a number of additional scenarios of practical interest.

  14. Clinical blood pool MR Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Leiner, Tim [Maastrich University Medical Center (Netherlands). Dept. of Radiology; Goyen, Martin [University Medical Center Hamburg-Eppendorf (Germany); Rohrer, Mathias [Bayer Schering Pharma AG, Berlin (Germany). European Business Unit Diagnostic Imaging; Schoenberg, Stefan O. (eds.) [University Hospital Mannheim Medical Faculty Mannheim - Heidelberg Univ. (Germany). Dept. of Clinical Radiology and Nuclear Medicine

    2008-07-01

    Clinical Blood Pool MR Imaging - This excellent treatise on Vasovist {sup registered} created by a team of exceptional faculty who are pioneers in MR Angiography covers the basic techniques, safety, efficacy, image processing and pharmaco-economic details to successfully implement a new level of MRA image quality with this new contrast agent. Martin Prince, Cornell University, New York The editors and authors have made groundbreaking contributions towards establishing MR angiography in various investigative settings, rendering it more precise and applying it for diverse indications. The work presented here is founded upon the extensive experience of the editors, as well as the broad range of experience from other scientific working groups. Maximilian Reiser, Ludwig Maximilian University, Munich Vasovist {sup registered} (Gadofosveset), worldwide the first blood pool agent, has only recently become available for clinical use, but has already gained wide acceptance as a tool to improve magnetic resonance angiography. This book presents the first in-depth introduction to the basic physicochemical aspects of the agent, the application of Vasovist {sup registered} in clinical MRA, as well as potential clinical applications beyond MRA and patient management-related aspects. The first part of the book explains basic and technical properties of the agent and the differences of Vasovist {sup registered} compared to currently available extracellular agents. The second part contains detailed chapters on safety and efficacy. In the third part the focus is on MR angiographic applications, and in the fourth part of the book potential clinical fields beyond MRA are explored. All clinical chapters feature ready-to-use clinical protocols and a series of take home messages that concisely summarize the current role of blood pool imaging for each specific indication. (orig.)

  15. Method of measuring a liquid pool volume

    Science.gov (United States)

    Garcia, G.V.; Carlson, N.M.; Donaldson, A.D.

    1991-03-19

    A method of measuring a molten metal liquid pool volume and in particular molten titanium liquid pools is disclosed, including the steps of (a) generating an ultrasonic wave at the surface of the molten metal liquid pool, (b) shining a light on the surface of a molten metal liquid pool, (c) detecting a change in the frequency of light, (d) detecting an ultrasonic wave echo at the surface of the molten metal liquid pool, and (e) computing the volume of the molten metal liquid. 3 figures.

  16. Large-scale pool fires

    Directory of Open Access Journals (Sweden)

    Steinhaus Thomas

    2007-01-01

    Full Text Available A review of research into the burning behavior of large pool fires and fuel spill fires is presented. The features which distinguish such fires from smaller pool fires are mainly associated with the fire dynamics at low source Froude numbers and the radiative interaction with the fire source. In hydrocarbon fires, higher soot levels at increased diameters result in radiation blockage effects around the perimeter of large fire plumes; this yields lower emissive powers and a drastic reduction in the radiative loss fraction; whilst there are simplifying factors with these phenomena, arising from the fact that soot yield can saturate, there are other complications deriving from the intermittency of the behavior, with luminous regions of efficient combustion appearing randomly in the outer surface of the fire according the turbulent fluctuations in the fire plume. Knowledge of the fluid flow instabilities, which lead to the formation of large eddies, is also key to understanding the behavior of large-scale fires. Here modeling tools can be effectively exploited in order to investigate the fluid flow phenomena, including RANS- and LES-based computational fluid dynamics codes. The latter are well-suited to representation of the turbulent motions, but a number of challenges remain with their practical application. Massively-parallel computational resources are likely to be necessary in order to be able to adequately address the complex coupled phenomena to the level of detail that is necessary.

  17. Structural integrity assessment of HANARO pool cover

    International Nuclear Information System (INIS)

    Ryu, Jeong Soo

    2001-11-01

    This report is for the seismic analysis and the structural integrity evaluation of HANARO Pool Cover in accordances with the requirement of the Technical Specification for Seismic Analysis of HANARO Pool Cover. For performing the seismic analysis and evaluating the structural integrity for HANARO Pool Cover, the finite element analysis model using ANSYS 5.7 was developed and the dynamic characteristics were analyzed. The seismic response spectrum analyses of HANARO Pool Cover under the design floor response spectrum loads of OBE and SSE were performed. The analysis results show that the stress values in HANARO Pool Cover for the seismic loads are within the ASME Code limits. It is also confirmed that the fatigue usage factor is less than 1.0. Therefore any damage on structural integrity is not expected when an HANARO Pool Cover is installed in the upper part of the reactor pool

  18. Bioreactor scale up and protein product quality characterization of piggyBac transposon derived CHO pools.

    Science.gov (United States)

    Rajendra, Yashas; Balasubramanian, Sowmya; Peery, Robert B; Swartling, James R; McCracken, Neil A; Norris, Dawn L; Frye, Christopher C; Barnard, Gavin C

    2017-03-01

    Chinese hamster ovary (CHO) cells remain the most popular host for the production of biopharmaceutical drugs, particularly monoclonal antibodies (mAbs), bispecific antibodies, and Fc-fusion proteins. Creating and characterizing the stable CHO clonally-derived cell lines (CDCLs) needed to manufacture these therapeutic proteins is a lengthy and laborious process. Therefore, CHO pools have increasingly been used to rapidly produce protein to support and enable preclinical drug development. We recently described the generation of CHO pools yielding mAb titers as high as 7.6 g/L in a 16 day bioprocess using piggyBac transposon-mediated gene integration. In this study, we wanted to understand why the piggyBac pool titers were significantly higher (2-10 fold) than the control CHO pools. Higher titers were the result of a combination of increased average gene copy number, significantly higher messenger RNA levels and the homogeneity (i.e. less diverse population distribution) of the piggyBac pools, relative to the control pools. In order to validate the use of piggyBac pools to support preclinical drug development, we then performed an in-depth product quality analysis of purified protein. The product quality of protein obtained from the piggyBac pools was very similar to the product quality profile of protein obtained from the control pools. Finally, we demonstrated the scalability of these pools from shake flasks to 36L bioreactors. Overall, these results suggest that gram quantities of therapeutic protein can be rapidly obtained from piggyBac CHO pools without significantly changing product quality attributes. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:534-540, 2017. © 2017 American Institute of Chemical Engineers.

  19. Microbial diversity in acidic thermal pools in the Uzon Caldera, Kamchatka.

    Science.gov (United States)

    Mardanov, Andrey V; Gumerov, Vadim M; Beletsky, Alexey V; Ravin, Nikolai V

    2018-01-01

    Microbial communities of four acidic thermal pools in the Uzon Caldera, Kamchatka, Russia, were studied using amplification and pyrosequencing of 16S rRNA gene fragments. The sites differed in temperature and pH: 1805 (60 °C, pH 3.7), 1810 (90 °C, pH 4.1), 1818 (80 °C, pH 3.5), and 1807 (86 °C, pH 5.6). Archaea of the order Sulfolobales were present among the dominant groups in all four pools. Acidilobales dominated in pool 1818 but were a minor fraction at the higher temperature in pool 1810. Uncultivated Archaea of the Hot Thaumarchaeota-related clade were present in significant quantities in pools 1805 and 1807, but they were not abundant in pools 1810 and 1818, where high temperatures were combined with low pH. Nanoarchaeota were present in all pools, but were more abundant in pools 1810 and 1818. A similar abundance pattern was observed for Halobacteriales. Thermophilic Bacteria were less diverse and were mostly represented by aerobic hydrogen- and sulfur-oxidizers of the phylum Aquificae and sulfur-oxidising Proteobacteria of the genus Acidithiobacillus. Thus we showed that extremely acidic hot pools contain diverse microbial communities comprising different metabolic groups of prokaryotes, including putative lithoautotrophs using energy sources of volcanic origin, and various facultative and obligate heterotrophs.

  20. Livermore pool-type reactor

    International Nuclear Information System (INIS)

    Mann, L.G.

    1977-01-01

    The Livermore Pool-Type Reactor (LPTR) has served a dual purpose since 1958--as an instrument for fundamental research and as a tool for measurement and calibration. Our early efforts centered on neutron-diffraction, fission, and capture gamma-ray studies. During the 1960's it was used for extensive calibration work associated with radiochemical and physical measurements on nuclear-explosive tests. Since 1970 the principal applications have been for trace-element measurements and radiation-damage studies. Today's research program is dominated by radiochemical studies of the shorter-lived fission products and by research on the mechanisms of radiation damage. Trace-element measurement for the National Uranium Resource Evaluation (NURE) program is the major measurement application today

  1. Cardiac blood pool emission tomography

    International Nuclear Information System (INIS)

    Itti, R.; Philippe, L.; Lorgeron, J.M.; Charbonnier, B.; Raynaud, P.; Brochier, M.

    1983-01-01

    After blood pool labeling using technetium-99m, a series of cardiac pictures is acquired during the rotation of a gamma-camera about the patient. Computer processing leads to reconstruction of various tomographic slices from the original planar projection. Electrocardiographic gating selects the different phases of the cardiac cycle. Individual slices through the left ventricular region are added in order to provide ''thick'' slices on which global and regional parameters of the left ventricular function can be determined. Due to the proportionality existing between count rates and labeled blood volumes, any geometrical model can be avoided. The delineation of regions of interest for count integration is made easier due to the absence of superimposition of structures; no correction for background is necessary. Tomography thus appears to be more consistent and more accurate than the classical methods using planar projections. In addition, right ventricular morphological and kinetic studies can be performed in the same conditions as for the left ventricle [fr

  2. Arc-weld pool interactions

    International Nuclear Information System (INIS)

    Glickstein, S.S.

    1978-08-01

    The mechanisms involved in arc-weld pool interactions are extremely complex and no complete theory is presently available to describe much of the phenomena observed during welding. For the past several years, experimental and analytical studies have been undertaken at the Bettis Atomic Power Laboratory to increase basic understanding of the gas tungsten arc welding process. These studies have included experimental spectral analysis of the arc in order to determine arc temperature and analytical modeling of the arc and weld puddle. The investigations have been directed toward determining the cause and effects of variations in the energy distribution incident upon the weldment. In addition, the effect of weld puddle distortion on weld penetration was investigated, and experimental and analytical studies of weld process variables have been undertaken to determine the effects of the variables upon weld penetration and configuration. A review of the results and analysis of these studies are presented

  3. Guide for decontaminating swimming pool at schools

    International Nuclear Information System (INIS)

    Matsuhashi, Shimpei; Kurikami, Hiroshi; Yasuda, Ryo; Takano, Takao; Seko, Noriaki; Naganawa, Hirochika; Kuroki, Ryota; Saegusa, Jun

    2012-07-01

    Because of TEPCO Fukushima Dai-ichi Nuclear Power Plant accident due to the Great East Japan Earthquake, a huge amount of radioactive materials was widely dispersed and precipitated into the environment. Swimming pools in Fukushima prefectures were contaminated with the radioactives. We JAEA carried out several demonstration tests to decontaminate the radioactives and discharge the pool water safely. We concluded the results obtained from the tests as 'Guide for decontaminating Swimming Pool at School' and released it quickly. Following this, we also released the guide in English. This manuscript, as an experimental report of the swimming pool water decontamination, is consisted from the guide in Japanese and English prepared. (author)

  4. Current operating practices of nuclear insurance pools

    International Nuclear Information System (INIS)

    O'Connell, J.M.

    1993-01-01

    This paper discusses the nuclear pooling system and co-operation between the pools, present practice and capacity, with a breakdown of the limits for third party liability and material damage. The author also describes the relationship between the pools and the nuclear operators (the policyholders), and concludes that the nuclear pools have been successful in serving the interests of their member companies, their policyholders and the governments as they have provided a stable insurance market by making available capacity in amounts that had never before been assembled and placed at risk in a single location. 2 tabs

  5. Guide for decontaminating swimming pool at schools

    Energy Technology Data Exchange (ETDEWEB)

    Matsuhashi, Shimpei; Kurikami, Hiroshi; Yasuda, Ryo; Takano, Takao; Seko, Noriaki; Naganawa, Hirochika; Kuroki, Ryota; Saegusa, Jun

    2012-07-15

    Because of TEPCO Fukushima Dai-ichi Nuclear Power Plant accident due to the Great East Japan Earthquake, a huge amount of radioactive materials was widely dispersed and precipitated into the environment. Swimming pools in Fukushima prefectures were contaminated with the radioactives. We JAEA carried out several demonstration tests to decontaminate the radioactives and discharge the pool water safely. We concluded the results obtained from the tests as 'Guide for decontaminating Swimming Pool at School' and released it quickly. Following this, we also released the guide in English. This manuscript, as an experimental report of the swimming pool water decontamination, is consisted from the guide in Japanese and English prepared. (author)

  6. Structure for nuclear fuel storage pools

    International Nuclear Information System (INIS)

    Ebata, Sakae; Nichiei, Shinji.

    1979-01-01

    Purpose: To enable leak detection in nuclear fuel storage pools, as well as prevent external leakages while keeping the strength of the constructional structures. Constitution: Protection plates are provided around pool linear plates and a leak reception is provided to the bottom. Leakages are detected by leak detecting pipeways and the external leakages are prevented by collecting them in a detection area provided in the intermediate layer. Since ferro-reinforcements at the bottom wall of the pool are disconnected by the protection plate making it impossible to form the constructional body, body hunches are provided to the bottom wall of the pool for processing the ferro-reinforcements. (Yoshino, Y.)

  7. Heat transfer from internally heated hemispherical pools

    International Nuclear Information System (INIS)

    Gabor, J.D.; Ellsion, P.G.; Cassulo, J.C.

    1980-01-01

    Experiments were conducted on heat transfer from internally heated ZnSO 4 -H 2 O pools to the walls of hemispherical containers. This experimental technique provides data for a heat transfer system that has to date been only theoretically treated. Three different sizes of copper hemispherical containers were used: 240, 280, 320 mm in diameter. The pool container served both as a heat transfer surface and as an electrode. The opposing electrode was a copper disk, 50 mm in diameter located at the top of the pool in the center. The top surface of the pool was open to the atmosphere

  8. Oxidation kinetics of corium pool

    Energy Technology Data Exchange (ETDEWEB)

    Sulatsky, A.A., E-mail: andrei314@mail.ru [Alexandrov Research Institute of Technologies (NITI), Sosnovy Bor (Russian Federation); Smirnov, S.A. [D.V. Efremov Scientific Research Institute of Electrophysical Apparatus (NIIEFA), St. Petersburg (Russian Federation); Granovsky, V.S.; Khabensky, V.B.; Krushinov, E.V.; Vitol, S.A.; Kotova, S.Yu. [Alexandrov Research Institute of Technologies (NITI), Sosnovy Bor (Russian Federation); Fischer, M.; Hellmann, S. [AREVA NP GmbH, Erlangen (Germany); Tromm, W.; Miassoedov, A. [Forschungzentrum Karlsruhe (FZK), Karlsruhe (Germany); Bottomley, D. [EUROPÄISCHE KOMMISSION, Joint Research Centre Institut für Transurane (ITU), Karlsruhe (Germany); Piluso, P. [CEA Cadarache-DEN/DTN/STRI, St.Paul-lez-Durance (France); Barrachin, M. [Institut de Radioprotection et Sûreté Nucléaire, St.Paul-lez-Durance (France)

    2013-09-15

    Highlights: • The analysis of experimental data on molten corium oxidation was been carried out. • The analysis has revealed the main factors influencing the oxidation kinetics. • The analysis was used for developing a qualitative analytical model. • The numerical modeling has confirmed the results of experimental data analysis. -- Abstract: Experimental, theoretical and numerical studies of oxidation kinetics of an open surface corium pool have been reported. The experiments have been carried out within OECD MASCA program and ISTC METCOR, METCOR-P and EVAN projects. It has been shown that the melt oxidation is controlled by an oxidant supply to the melt free surface from the atmosphere, not by the reducer supply from the melt. The project experiments have not detected any input of the zirconium oxidation kinetics into the process chemistry. The completed analysis puts forward a simple analytical model, which gives an explanation of the main features of melt oxidation process. The numerical modeling results are in good agreement with experimental data and theoretical considerations.

  9. Oxidation kinetics of corium pool

    International Nuclear Information System (INIS)

    Sulatsky, A.A.; Smirnov, S.A.; Granovsky, V.S.; Khabensky, V.B.; Krushinov, E.V.; Vitol, S.A.; Kotova, S.Yu.; Fischer, M.; Hellmann, S.; Tromm, W.; Miassoedov, A.; Bottomley, D.; Piluso, P.; Barrachin, M.

    2013-01-01

    Highlights: • The analysis of experimental data on molten corium oxidation was been carried out. • The analysis has revealed the main factors influencing the oxidation kinetics. • The analysis was used for developing a qualitative analytical model. • The numerical modeling has confirmed the results of experimental data analysis. -- Abstract: Experimental, theoretical and numerical studies of oxidation kinetics of an open surface corium pool have been reported. The experiments have been carried out within OECD MASCA program and ISTC METCOR, METCOR-P and EVAN projects. It has been shown that the melt oxidation is controlled by an oxidant supply to the melt free surface from the atmosphere, not by the reducer supply from the melt. The project experiments have not detected any input of the zirconium oxidation kinetics into the process chemistry. The completed analysis puts forward a simple analytical model, which gives an explanation of the main features of melt oxidation process. The numerical modeling results are in good agreement with experimental data and theoretical considerations

  10. 13 CFR 120.1708 - Pool Certificates.

    Science.gov (United States)

    2010-01-01

    ... United States. (c) SBA purchase of a Loan Interest. SBA will determine whether to purchase a Loan... reserves the right to purchase a Loan Interest from a Pool at any time. (d) Self-liquidating. A Pool... market conditions and program experience, and will publish any such change in the Federal Register. (h...

  11. Compact fuel storage rack for fuel pools

    International Nuclear Information System (INIS)

    Parras, F.; Louvat, J.P.

    1986-01-01

    ETS LEMER and FRAMATOME propose a new compact storage rack. This rack permits a considerable increase of the storage capacity of cooling pools. A short description of the structure and the components is presented, to propose racks that are: . Inalterable, . Compact, . Insensitive to earthquakes. Installation in pools already in operation is simplified by their light structure and the bearing device [fr

  12. The warm pool in the Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Vinayachandran, P.N.; Shetye, S.R.

    is larger and warmer, a peculiarity of the pool in the Indian Ocean is its seasonal variation. The surface area of the pool changes from 24 x 106 km2 in April to 8 x 106 km2 in September due to interaction with the southwest monsoon. The annual cycles of sea...

  13. Shedding Light on Dark Liquidity Pools

    NARCIS (Netherlands)

    Degryse, H.A.; van Achter, M.; Wuyts, G.

    2008-01-01

    This article reviews the recent developments on dark liquidity pools starting from the theoretical and empirical academic literature. The number of dark liquidity pools as well as their trading volume has grown substantially in the last couple of years. We highlight the incentives of providers as

  14. Big city consultants shut down our pool : a shocking community pool gets checked for stray voltage

    Energy Technology Data Exchange (ETDEWEB)

    Lynch, P. [Power Line Systems Engineering Inc., Markham, ON (Canada)

    2009-12-15

    This article discussed an investigation conducted at a community pool where swimmers complained of receiving electrical shocks both in the pool and on the pool's deck area. Electrical measurements taken at the pool revealed current flows from the pool water to various points around the deck area. Measured current flow in the pool area was 30 amps even when the main pool service breaker was opened to shut off power to the entire facility. Thirty amps of primary neutral current was then measured on the primary side aerial neutral in front of the pool. A 10 amp primary feeder from the pool joined up with the complex's primary neutral wire to increase the neutral current to 40 amps. The combined 40 amps current then returned to the secondary side of a nearby utility transformer substation. The study showed that the underground wet low-resistance grounded surface area of the pool was attracting the 30 amps of utility current from the surrounding ground area. The local utility disconnected the primary and secondary neutral interconnection at the pool's main 600-volt step-down transformer. The pool deck was removed in order to install additional copper bonding grounds. In order to avert serious injuries, many experts propose that all electric utilities should be required by law to reconfigure their power systems to prevent primary power neutral currents from entering private buildings. 1 tab., 2 figs.

  15. Ripples in a superconducting tidal pool

    CERN Document Server

    Hudson, E

    2003-01-01

    The behaviour of electrons in a metal is often compared to that of water in a pool. An empty pool is like a material that has all of its electrons removed. As electrons are 'poured' into the metal, they first occupy the lowest energies available - the bottom of the pool - and eventually fill up to the Fermi energy, the top of the pool. At this point we no longer discuss electrons but quasiparticles. These are electrons that have modified properties due to their interactions within the material. Waves in a pool can be excited, and their properties will depend on the depth of the water. Similarly in a metal, quasiparticles behave like waves that have a material-dependent dispersion relation between their energy and their wavevector, which specifies their direction and wavelength. This simple analogy also hints at an indirect method of measuring the dispersion relation of a metal, and hence the myriad of properties that depend on it. (U.K.)

  16. Design and Construction of Pool Door for Research Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Kwangsub; Lee, Sangjin; Choi, Jinbok; Oh, Jinho; Lee, Jongmin [KAERI, Daejeon (Korea, Republic of)

    2016-05-15

    The pool door is a structure to isolate the reactor pool from the service pool for maintenance. The pool door is installed before the reactor pool is drained. The pool door consists of structural component and sealing component. The main structures of the pool door are stainless steel plates and side frames. The plates and frames are assembled by welded joints. Lug is welded at the top of the plate. The pool door is submerged in the pool water when it is used. Materials of the pool door should be resistive to corrosion and radiation. Stainless steel is used in structural components and air nozzle assemblies. Features of design and construction of the pool door for the research reactor are introduced. The pool door is designed to isolate the reactor pool for maintenance. Structural analysis is performed to evaluate the structural integrity during earthquake. Tests and inspections are also carried out during construction to identify the safety and function of the pool door.

  17. Design and Construction of Pool Door for Research Reactor

    International Nuclear Information System (INIS)

    Jung, Kwangsub; Lee, Sangjin; Choi, Jinbok; Oh, Jinho; Lee, Jongmin

    2016-01-01

    The pool door is a structure to isolate the reactor pool from the service pool for maintenance. The pool door is installed before the reactor pool is drained. The pool door consists of structural component and sealing component. The main structures of the pool door are stainless steel plates and side frames. The plates and frames are assembled by welded joints. Lug is welded at the top of the plate. The pool door is submerged in the pool water when it is used. Materials of the pool door should be resistive to corrosion and radiation. Stainless steel is used in structural components and air nozzle assemblies. Features of design and construction of the pool door for the research reactor are introduced. The pool door is designed to isolate the reactor pool for maintenance. Structural analysis is performed to evaluate the structural integrity during earthquake. Tests and inspections are also carried out during construction to identify the safety and function of the pool door

  18. Pool scrubbing models for iodine components

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, K [Battelle Ingenieurtechnik GmbH, Eschborn (Germany)

    1996-12-01

    Pool scrubbing is an important mechanism to retain radioactive fission products from being carried into the containment atmosphere or into the secondary piping system. A number of models and computer codes has been developed to predict the retention of aerosols and fission product vapours that are released from the core and injected into water pools of BWR and PWR type reactors during severe accidents. Important codes in this field are BUSCA, SPARC and SUPRA. The present paper summarizes the models for scrubbing of gaseous Iodine components in these codes, discusses the experimental validation, and gives an assessment of the state of knowledge reached and the open questions which persist. The retention of gaseous Iodine components is modelled by the various codes in a very heterogeneous manner. Differences show up in the chemical species considered, the treatment of mass transfer boundary layers on the gaseous and liquid sides, the gas-liquid interface geometry, calculation of equilibrium concentrations and numerical procedures. Especially important is the determination of the pool water pH value. This value is affected by basic aerosols deposited in the water, e.g. Cesium and Rubidium compounds. A consistent model requires a mass balance of these compounds in the pool, thus effectively coupling the pool scrubbing phenomena of aerosols and gaseous Iodine species. Since the water pool conditions are also affected by drainage flow of condensate water from different regions in the containment, and desorption of dissolved gases on the pool surface is determined by the gas concentrations above the pool, some basic limitations of specialized pool scrubbing codes are given. The paper draws conclusions about the necessity of coupling between containment thermal-hydraulics and pool scrubbing models, and proposes ways of further simulation model development in order to improve source term predictions. (author) 2 tabs., refs.

  19. Pool scrubbing models for iodine components

    International Nuclear Information System (INIS)

    Fischer, K.

    1996-01-01

    Pool scrubbing is an important mechanism to retain radioactive fission products from being carried into the containment atmosphere or into the secondary piping system. A number of models and computer codes has been developed to predict the retention of aerosols and fission product vapours that are released from the core and injected into water pools of BWR and PWR type reactors during severe accidents. Important codes in this field are BUSCA, SPARC and SUPRA. The present paper summarizes the models for scrubbing of gaseous Iodine components in these codes, discusses the experimental validation, and gives an assessment of the state of knowledge reached and the open questions which persist. The retention of gaseous Iodine components is modelled by the various codes in a very heterogeneous manner. Differences show up in the chemical species considered, the treatment of mass transfer boundary layers on the gaseous and liquid sides, the gas-liquid interface geometry, calculation of equilibrium concentrations and numerical procedures. Especially important is the determination of the pool water pH value. This value is affected by basic aerosols deposited in the water, e.g. Cesium and Rubidium compounds. A consistent model requires a mass balance of these compounds in the pool, thus effectively coupling the pool scrubbing phenomena of aerosols and gaseous Iodine species. Since the water pool conditions are also affected by drainage flow of condensate water from different regions in the containment, and desorption of dissolved gases on the pool surface is determined by the gas concentrations above the pool, some basic limitations of specialized pool scrubbing codes are given. The paper draws conclusions about the necessity of coupling between containment thermal-hydraulics and pool scrubbing models, and proposes ways of further simulation model development in order to improve source term predictions. (author) 2 tabs., refs

  20. Operation and maintenance techniques of pool and pool water purification system in IMEF

    Energy Technology Data Exchange (ETDEWEB)

    Soong, Woong Sup

    1999-03-01

    IMEF pool is used pass way between pool and hot cell in order to inlet and outlet of fuel pin in cask. All operation is performed conforming with naked eyes. Therefore floating matter is filtered so as to easy under water handling. Also radioactivity in pool water is controlled according to the nuclear law, radioactivity ration maintained less than 15mR/hr on pool side. Perfect operation and maintenance can be achieved well trained operator. Result obtained from the perfection can give more influence over restrain, spreading contamination of radioactivity materials. This report describes operation and maintenance technique of pool water purification system in IMEF. (Author). 7 refs., 13 figs.

  1. Operation and maintenance techniques of pool and pool water purification system in IMEF

    International Nuclear Information System (INIS)

    Soong, Woong Sup

    1999-03-01

    IMEF pool is used pass way between pool and hot cell in order to inlet and outlet of fuel pin in cask. All operation is performed conforming with naked eyes. Therefore floating matter is filtered so as to easy under water handling. Also radioactivity in pool water is controlled according to the nuclear law, radioactivity ration maintained less than 15mR/hr on pool side. Perfect operation and maintenance can be achieved well trained operator. Result obtained from the perfection can give more influence over restrain, spreading contamination of radioactivity materials. This report describes operation and maintenance technique of pool water purification system in IMEF. (Author). 7 refs., 13 figs

  2. Swimming pool hydraulics and their significance for public pools. Bedeutung der Beckenhydraulik in oeffentlichen Schwimmbaedern

    Energy Technology Data Exchange (ETDEWEB)

    Gansloser, G

    1989-11-01

    The term of swimming pool hydraulics means the process of letting in and drawing off water to and from the pool while ensuring that no inadmissible water-borne contaminant concentrations will occur anywhere within the pool. Measurements were performed on a pool to study the significance of correct pool hydraulics. The author points out that a wrong water recirculation design will bring to nought the effects of an elaborate water treatment system; by contrast, poor pool water quality can be greatly improved by redesigning the pool water hydraulics approach. In principle, systems with with water inlet at one side and water outlet at the far side will fall short of hygienic requirements. (BWI).

  3. Studies of thermal stratification in water pool

    International Nuclear Information System (INIS)

    Verma, P.K.; Chandraker, D.K.; Nayak, A.K.; Vijayan, P.K.

    2015-01-01

    Large water pools are used as a heat sink for various cooling systems used in industry. In context of advance nuclear reactors like AHWR, it is used as ultimate heat sink for passive systems for decay heat removal and containment cooling. This system incorporates heat exchangers submerged in the large water pool. However, heat transfer by natural convection in pool poses a problem of thermal stratification. Due to thermal stratification hot layers of water accumulate over the relatively cold one. The heat transfer performance of heat exchanger gets deteriorated as a hot fluid envelops it. In the nuclear reactors, the walls of the pool are made of concrete and it may subject to high temperature due to thermal stratification which is not desirable. In this paper, a concept of employing shrouds around the heat source is studied. These shrouds provide a bulk flow in the water pool, thereby facilitating mixing of hot and cold fluid, which eliminate stratification. The concept has been applied to the a scaled model of Gravity Driven Water Pool (GDWP) of AHWR in which Isolation Condensers (IC) tubes are submerged for decay heat removal of AHWR using ICS and thermal stratification phenomenon was predicted with and without shrouds. To demonstrate the adequacy of the effectiveness of shroud arrangement and to validate the simulation methodology of RELAP5/Mod3.2, experiments has been conducted on a scaled model of the pool with and without shroud. (author)

  4. Spent fuel pool cleanup and stabilization

    International Nuclear Information System (INIS)

    Miller, R.L.

    1987-06-01

    Each of the plutonium production reactors at Hanford had a large water-filled spent fuel pool to provide interim storage of irradiated fuel while awaiting shipment to the separation facilities. After cessation of reactor operations the fuel was removed from the pools and the water levels were drawn down to a 5- to 10-foot depth. The pools were maintained with the water to provide shielding and radiological control. What appeared to be a straightforward project to process the water, remove the sediments from the basin, and stabilize the contamination on the floors and walls became a very complex and time consuming operation. The sediment characteristics varied from pool to pool, the ion exchange system required modification, areas of hard-pack sediments were discovered on the floors, special arrangements to handle and package high dose rate items for shipment were required, and contract problems ensued with the subcontractor. The original schedule to complete the project from preliminary engineering to final stabilization of the pools was 15 months. The actual time required was about 25 months. The original cost estimate to perform the work was $2,651,000. The actual cost of the project was $5,120,000, which included $150,000 for payment of claims to the subcontractor. This paper summarizes the experiences associated with the cleanup and radiological stabilization of the 100-B, -C, -D, and -DR spent fuel pools, and discusses a number of lessons learned items

  5. Distributed Technologies in a Data Pool

    Science.gov (United States)

    Keiser, K.; Conover, H.; Graves, S.; He, Y.; Regner, K.; Smith, M.

    2004-12-01

    A Data Pool is an on-line repository providing interactive and programmatic access to data products through a variety of services. The University of Alabama in Huntsville has developed and deployed such a Data Pool in conjunction with the DISCOVER project, a collaboration with NASA and Remote Sensing Systems. DISCOVER provides long-term ocean and climate data from a variety of passive microwave satellite instruments, including such products as sea-surface temperature and wind, air temperature, atmospheric water vapor, cloud water and rain rate. The Data Pool provides multiple methods to access and visualize these products, including conventional HTTP and FTP access, as well as data services that provide for enhanced usability and interoperability, such as GridFTP, OPeNDAP, OpenGIS-compliant web mapping and coverage services, and custom subsetting and packaging services. This paper will focus on the distributed service technologies used in the Data Pool, which spans heterogeneous machines at multiple locations. For example, in order to provide seamless access to data at multiple sites, the Data Pool provides catalog services for all data products at the various data server locations. Under development is an automated metadata generation tool that crawls the online data repositories regularly to dynamically update the Data Pool catalog with information about newly generated data files. For efficient handling of data orders across distributed repositories, the Data Pool also implements distributed data processing services on the file servers where the data resides. Ontologies are planned to support automated service chaining for custom user requests. The UAH Data Pool is based on a configurable technology framework that integrates distributed data services with a web interface and a set of centralized database services for catalogs and order tracking. While this instantiation of the Data Pool was implemented to meet the needs of the DISCOVER project, the framework was

  6. A Pool of Distant Galaxies

    Science.gov (United States)

    2008-11-01

    Anyone who has wondered what it might be like to dive into a pool of millions of distant galaxies of different shapes and colours, will enjoy the latest image released by ESO. Obtained in part with the Very Large Telescope, the image is the deepest ground-based U-band image of the Universe ever obtained. It contains more than 27 million pixels and is the result of 55 hours of observations with the VIMOS instrument. A Sea of Galaxies ESO PR Photo 39/08 A Pool of Distant Galaxies This uniquely beautiful patchwork image, with its myriad of brightly coloured galaxies, shows the Chandra Deep Field South (CDF-S), arguably the most observed and best studied region in the entire sky. The CDF-S is one of the two regions selected as part of the Great Observatories Origins Deep Survey (GOODS), an effort of the worldwide astronomical community that unites the deepest observations from ground- and space-based facilities at all wavelengths from X-ray to radio. Its primary purpose is to provide astronomers with the most sensitive census of the distant Universe to assist in their study of the formation and evolution of galaxies. The new image released by ESO combines data obtained with the VIMOS instrument in the U- and R-bands, as well as data obtained in the B-band with the Wide-Field Imager (WFI) attached to the 2.2 m MPG/ESO telescope at La Silla, in the framework of the GABODS survey. The newly released U-band image - the result of 40 hours of staring at the same region of the sky and just made ready by the GOODS team - is the deepest image ever taken from the ground in this wavelength domain. At these depths, the sky is almost completely covered by galaxies, each one, like our own galaxy, the Milky Way, home of hundreds of billions of stars. Galaxies were detected that are a billion times fainter than the unaided eye can see and over a range of colours not directly observable by the eye. This deep image has been essential to the discovery of a large number of new galaxies

  7. Modal analysis of pool door in water tank

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kang Soo; Jeong, Kyeong Hoon; Park, Chan Gook; Koo, In Soo [KAERI, Daejeon (Korea, Republic of)

    2012-10-15

    A pool door is installed at the chase of the pool gate by means of an overhead crane in the building of a research reactor. The principal function of the pool door, which is located between the reactor pool and service pool, is to separate the reactor pool from the service pool for the maintenance and/or the removal of the equipment either in the reactor pool or service pool. The pool door consists of stainless steel plates supported by structural steel frames and sealing components. The pool door is equipped with double inflatable gaskets. The configuration of the pool door is shown in Figure 1. The FEM analysis and theoretical calculation by the formula were performed to evaluate the natural frequency for the pool door in the water. The results from the two methods were compared.

  8. The Role of Nuclear Insurance Pools

    International Nuclear Information System (INIS)

    Reitsma, S. M. S.

    2006-01-01

    Since fifty years insurers respond to the need of both governments and the electricity industry to provide financial protection to cover the perils presented by the use of nuclear energy for peaceful purposes. This paper aims at explaining what difficulties had to be solved in order to enable insurers to provide such protection, that as a solution to these difficulties Nuclear Insurance Pools were formed, how such pools operate and what security they provide. Thereby not only a number of universal principles underlying nuclear pool insurance will be explained, but also some differences in the characteristics of such insurance per group of countries. (author)

  9. Laser surveillance systems for fuel storage pools

    International Nuclear Information System (INIS)

    Boeck, H.

    1985-06-01

    A Laser Surveillance System (LASSY) as a new safeguards device has been developed under the IAEA research contract No. 3458/RB at the Atominstitut Wien using earlier results by S. Fiarman. This system is designed to act as a sheet of light covering spent fuel assemblies in spent fuel storage pools. When movement of assemblies takes place, LASSY detects and locates the position of the movement in the pool and when interrogated, presents a list of pool positions and times of movement to the safeguards inspector. A complete prototype system was developed and built. Full scale tests showed the principal working capabilities of a LASSY underwater

  10. Genotyping common and rare variation using overlapping pool sequencing

    Directory of Open Access Journals (Sweden)

    Pasaniuc Bogdan

    2011-07-01

    Full Text Available Abstract Background Recent advances in sequencing technologies set the stage for large, population based studies, in which the ANA or RNA of thousands of individuals will be sequenced. Currently, however, such studies are still infeasible using a straightforward sequencing approach; as a result, recently a few multiplexing schemes have been suggested, in which a small number of ANA pools are sequenced, and the results are then deconvoluted using compressed sensing or similar approaches. These methods, however, are limited to the detection of rare variants. Results In this paper we provide a new algorithm for the deconvolution of DNA pools multiplexing schemes. The presented algorithm utilizes a likelihood model and linear programming. The approach allows for the addition of external data, particularly imputation data, resulting in a flexible environment that is suitable for different applications. Conclusions Particularly, we demonstrate that both low and high allele frequency SNPs can be accurately genotyped when the DNA pooling scheme is performed in conjunction with microarray genotyping and imputation. Additionally, we demonstrate the use of our framework for the detection of cancer fusion genes from RNA sequences.

  11. 10 CFR 36.63 - Pool water purity.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Pool water purity. 36.63 Section 36.63 Energy NUCLEAR... § 36.63 Pool water purity. (a) Pool water purification system must be run sufficiently to maintain the conductivity of the pool water below 20 microsiemens per centimeter under normal circumstances. If pool water...

  12. Impact and quantification of the sources of error in DNA pooling designs.

    Science.gov (United States)

    Jawaid, A; Sham, P

    2009-01-01

    The analysis of genome wide variation offers the possibility of unravelling the genes involved in the pathogenesis of disease. Genome wide association studies are also particularly useful for identifying and validating targets for therapeutic intervention as well as for detecting markers for drug efficacy and side effects. The cost of such large-scale genetic association studies may be reduced substantially by the analysis of pooled DNA from multiple individuals. However, experimental errors inherent in pooling studies lead to a potential increase in the false positive rate and a loss in power compared to individual genotyping. Here we quantify various sources of experimental error using empirical data from typical pooling experiments and corresponding individual genotyping counts using two statistical methods. We provide analytical formulas for calculating these different errors in the absence of complete information, such as replicate pool formation, and for adjusting for the errors in the statistical analysis. We demonstrate that DNA pooling has the potential of estimating allele frequencies accurately, and adjusting the pooled allele frequency estimates for differential allelic amplification considerably improves accuracy. Estimates of the components of error show that differential allelic amplification is the most important contributor to the error variance in absolute allele frequency estimation, followed by allele frequency measurement and pool formation errors. Our results emphasise the importance of minimising experimental errors and obtaining correct error estimates in genetic association studies.

  13. The Nuclear Insurance Pools: Operations and Covers

    International Nuclear Information System (INIS)

    Tetley, M.

    2008-01-01

    Nuclear insurance pools have provided insurance for the nuclear industry for over fifty years and it is fair to say that the development of civil nuclear power would not have been possible without the support of the commercial insurance market. The unknown risks presented by the nascent nuclear power industry in the 1950s required a leap of faith by insurers who developed specialist pooled insurance capacity to ensure adequate capacity to back up the operators' compensation obligations. Since then, nuclear insurance pools have evolved to become comprehensive suppliers of most types of insurance for nuclear plant globally. This paper will outline the structure, development, products and current operations of nuclear insurance pools.(author)

  14. Ingestion of swimming pool water by recreational

    Data.gov (United States)

    U.S. Environmental Protection Agency — Swimming pool water ingestion data. This dataset is associated with the following publication: Dufour, A., L. Wymer, M. Magnuson, T. Behymer, and R. Cantu. Ingestion...

  15. Nuclear Insurance Pools: Worldwide Practice and Development

    International Nuclear Information System (INIS)

    Reitsma, S. M. S.

    1998-01-01

    The development of nuclear installations to produce electricity led to the establishment of Nuclear Insurance Pools and the introduction of international Conventions on Third Party Liability. Nuclear Pools offer both Third Party Liability insurance, reflecting the Conventions' principles, and other insurance products. They are market-wide, providing a facility for participation by insurers who could not otherwise write the insurance for the particularly sensitive nuclear risk. All acceptances are for the net retention of each Member without recourse to individual reinsurance protection. Common account reinsurance is arranged with other Nuclear Pools all over the world. Thus, a transparency is created, which ensures the highest degree of reinsurance security and imposes a known finite limit to each participating insurer's commitment. Therefore, Pool-members are prepared to make a greater commitment to nuclear risks than would be case where they felt uncertain as regards their total exposure following a significant loss. (author)

  16. AE/VCE Unconfirmed Vernal Pools

    Data.gov (United States)

    Vermont Center for Geographic Information — This dataset is derived from a project by the Vermont Center for Ecostudies(VCE) and Arrowwood Environmental(AE) to map vernal pools throughout the state of Vermont....

  17. AE/VCE Confirmed Vernal Pools

    Data.gov (United States)

    Vermont Center for Geographic Information — This dataset is derived from a project by the Vermont Center for Ecostudies(VCE) and Arrowwood Environmental(AE) to map vernal pools throughout the state of Vermont....

  18. Investigations in Marine Chemistry: Tide Pool Ecology.

    Science.gov (United States)

    Schlenker, Richard M.

    Students investigated the salinity of tide pools at different levels in the intertidal zone. Data are analyzed collectively. Students graphed and discussed data. Included are suggestions for evaluation and further study. (Author)

  19. 7 CFR 1126.7 - Pool plant.

    Science.gov (United States)

    2010-01-01

    ... excluded from the supply plant's shipments in computing the plant's shipping percentage. (d) A plant... part, or the plant has automatic pooling status under the other Federal order; and (7) That portion of...

  20. 7 CFR 1001.7 - Pool plant.

    Science.gov (United States)

    2010-01-01

    ... excluded from the supply plant's shipments in computing the percentages in paragraphs (c)(1) and (2) of... plant has automatic pooling status under the other Federal order. [64 FR 47954, Sept. 1, 1999, as...

  1. 7 CFR 1007.7 - Pool plant.

    Science.gov (United States)

    2010-01-01

    ...-upon use other than Class I shall be excluded from the supply plant's shipments in computing the plant... under the order in this part, or such plant has automatic pooling status under such other order. (h) Any...

  2. Microbial quality of a marine tidal pool

    CSIR Research Space (South Africa)

    Genthe, Bettina

    1995-01-01

    Full Text Available In this study the source of microbial pollution to a tidal pool was investigated. Both adjacent seawater which could contribute to possible faecal pollution and potential direct bather pollution were studied. The microbial quality of the marine...

  3. Robotic cleaning of a spent fuel pool

    International Nuclear Information System (INIS)

    Roman, H.T.; Marian, F.A.; Silverman, E.B.; Barkley, V.P.

    1987-01-01

    Spent fuel pools at nuclear power plants are not cleaned routinely, other than by purifying the water that they contain. Yet, debris can collect on the bottom of a pool and should be removed prior to fuel transfer. At Public Service Electric and Gas Company's Hope Creek Nuclear Power Plant, a submersible mobile robot - ARD Corporation's SCAVENGER - was used to clean the bottom of the spent fuel pool prior to initial fuel loading. The robotic device was operated remotely (as opposed to autonomously) with a simple forward/reverse control, and it cleaned 70-80% of the pool bottom. This paper reports that a simple cost-benefit analysis shows that the robotic device would be less expensive, on a per mission basis, than other cleaning alternatives, especially if it were used for other similar cleaning operations throughout the plant

  4. Pooling strategies for St Petersburg gamblers

    OpenAIRE

    Csörgö, Sandor; Simons, Gordon

    2006-01-01

    Peter offers to play exactly one St Petersburg game with each of [math] players, Paul [math] , [math] , Paul [math] , whose conceivable pooling strategies are described by all possible probability distributions [math] . Comparing infinite expectations, we characterize among all [math] those admissible strategies for which the pooled winnings, each distributed as [math] , yield a finite added value for each and every one of Paul [math] , [math] , Paul [math] in comparison with their individual...

  5. Welding pool measurement using thermal array sensor

    Science.gov (United States)

    Cho, Chia-Hung; Hsieh, Yi-Chen; Chen, Hsin-Yi

    2015-08-01

    Selective laser melting (SLM) is an additive manufacturing (AM) technology that uses a high-power laser beam to melt metal powder in chamber of inert gas. The process starts by slicing the 3D CAD data as a digital information source into layers to create a 2D image of each layer. Melting pool was formed by using laser irradiation on metal powders which then solidified to consolidated structure. In a selective laser melting process, the variation of melt pool affects the yield of a printed three-dimensional product. For three dimensional parts, the border conditions of the conductive heat transport have a very large influence on the melt pool dimensions. Therefore, melting pool is an important behavior that affects the final quality of the 3D object. To meet the temperature and geometry of the melting pool for monitoring in additive manufacturing technology. In this paper, we proposed the temperature sensing system which is composed of infrared photodiode, high speed camera, band-pass filter, dichroic beam splitter and focus lens. Since the infrared photodiode and high speed camera look at the process through the 2D galvanometer scanner and f-theta lens, the temperature sensing system can be used to observe the melting pool at any time, regardless of the movement of the laser spot. In order to obtain a wide temperature detecting range, 500 °C to 2500 °C, the radiation from the melting pool to be measured is filtered into a plurality of radiation portions, and since the intensity ratio distribution of the radiation portions is calculated by using black-body radiation. The experimental result shows that the system is suitable for melting pool to measure temperature.

  6. Numerical modelling of methanol liquid pool fires

    Science.gov (United States)

    Prasad, Kuldeep; Li, Chiping; Kailasanath, K.; Ndubizu, Chuka; Ananth, Ramagopal; Tatem, P. A.

    1999-12-01

    The focus of this paper is on numerical modelling of methanol liquid pool fires. A mathematical model is first developed to describe the evaporation and burning of a two-dimensional or axisymmetric pool containing pure liquid methanol. Then, the complete set of unsteady, compressible Navier-Stokes equations for reactive flows are solved in the gas phase to describe the convection of the fuel gases away from the pool surface, diffusion of the gases into the surrounding air and the oxidation of the fuel into product species. Heat transfer into the liquid pool and the metal container through conduction, convection and radiation are modelled by solving a modified form of the energy equation. Clausius-Clapeyron relationships are invoked to model the evaporation rate of a two-dimensional pool of pure liquid methanol. The governing equations along with appropriate boundary and interface conditions are solved using the flux-corrected transport algorithm. Numerical results exhibit a flame structure that compares well with experimental observations. Temperature profiles and burning rates were found to compare favourably with experimental data from single- and three-compartment laboratory burners. The model predicts a puffing frequency of approximately 12 Hz for a 1 cm diameter methanol pool in the absence of any air co-flow. It is also observed that increasing the air co-flow velocity helps in stabilizing the diffusion flame, by pushing the vortical structures away from the flame region.

  7. Recent advances in probabilistic species pool delineations

    Directory of Open Access Journals (Sweden)

    Dirk Nikolaus Karger

    2016-07-01

    Full Text Available A species pool is the set of species that could potentially colonize and establish within a community. It has been a commonly used concept in biogeography since the early days of MacArthur and Wilson’s work on Island Biogeography. Despite their simple and appealing definition, an operational application of species pools is bundled with a multitude of problems, which have often resulted in arbitrary decisions and workarounds when defining species pools. Two recently published papers address the operational problems of species pool delineations, and show ways of delineating them in a probabilistic fashion. In both papers, species pools were delineated using a process-based, mechanistical approach, which opens the door for a multitude of new applications in biogeography. Such applications include detecting the hidden signature of biotic interactions, disentangling the geographical structure of community assembly processes, and incorporating a temporal extent into species pools. Although similar in their conclusions, both ‘probabilistic approaches’ differ in their implementation and definitions. Here I give a brief overview of the differences and similarities of both approaches, and identify the challenges and advantages in their application.

  8. Recombinant protein production from stable mammalian cell lines and pools.

    Science.gov (United States)

    Hacker, David L; Balasubramanian, Sowmya

    2016-06-01

    We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site-specific recombinases (targeted transgene integration). Each of these methods results in the generation of cell lines with protein yields that are generally superior to those achievable through classical plasmid transfection that depends on the integration of the transfected DNA by non-homologous DNA end-joining. This is the main reason why these techniques can also be used for the generation of stable cell pools, heterogenous populations of recombinant cells generated by gene delivery and genetic selection without resorting to single cell cloning. This allows the time line from gene transfer to protein production to be reduced. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Self-formed waterfall plunge pools in homogeneous rock

    Science.gov (United States)

    Scheingross, Joel S.; Lo, Daniel Y.; Lamb, Michael P.

    2017-01-01

    Waterfalls are ubiquitous, and their upstream propagation can set the pace of landscape evolution, yet no experimental studies have examined waterfall plunge pool erosion in homogeneous rock. We performed laboratory experiments, using synthetic foam as a bedrock simulant, to produce self-formed waterfall plunge pools via particle impact abrasion. Plunge pool vertical incision exceeded lateral erosion by approximately tenfold until pools deepened to the point that the supplied sediment could not be evacuated and deposition armored the pool bedrock floor. Lateral erosion of plunge pool sidewalls continued after sediment deposition, but primarily at the downstream pool wall, which might lead to undermining of the plunge pool lip, sediment evacuation, and continued vertical pool floor incision in natural streams. Undercutting of the upstream pool wall was absent, and our results suggest that vertical drilling of successive plunge pools is a more efficient waterfall retreat mechanism than the classic model of headwall undercutting and collapse in homogeneous rock.

  10. Structure of pool in reactor building

    International Nuclear Information System (INIS)

    Yokoyama, Shigeki.

    1997-01-01

    Shielding walls made of iron-reinforced concrete having a metal liner including two body walls rigidly combined to the upper surface of a reactor container are disposed at least to one of an equipment pool or spent fuel storage pool in a reactor building. A rack for temporarily placing an upper lattice plate is detachably attached at least above one of a steam dryer or a gas/liquid separator temporarily placed in the temporary pool, and the height from the bottom portion to the upper end of the shielding wall is determined based on the height of an upper lattice plate temporary placed on the rack and the water depth required for shielding radiation from the upper lattice plate. An operator's exposure on the operation floor can be reduced by the shielding wall, and radiation dose from the spent fuels is reduced. The increase of the height of a pool guarder enhances bending resistance as a ceiling. In addition, the total height of them is made identical with the depth of the spent fuel storage pool thereby enabling to increase storage area for spent fuels. (N.H.)

  11. Cooling device for reactor suppression pool

    International Nuclear Information System (INIS)

    Togasaki, Susumu; Kato, Kiyoshi.

    1994-01-01

    In a cooling device of a reactor suppression pool, when a temperature of pool water is abnormally increased and a heat absorbing portion is heated by, for example, occurrence of an accident, coolants are sent to the outside of the reactor container to actuates a thermally operating portion by the heat energy of coolants and drive heat exchanging fluids of a secondary cooling system. If the heat exchanging fluids are sent to a cooling portion, the coolants are cooled and returned to the heat absorbing portion of the suppression pool water. If the heat absorbing portion is heat pipes, the coolants are evaporated by heat absorbed from the suppression pool water, steams are sent to the thermally operating portion, then coolants are liquefied and caused to return to the heat absorbing portion. If the thermal operation portion is a gas turbine, the gas turbine is operated by the coolants, and it is converted to a rotational force to drive heat exchanging fluids by pumps. By constituting the cooling portion with a condensator, the coolants are condensed and liquefied and returned to the heat absorbing portion of the suppression pool water. (N.H.)

  12. Condensation in a two-phase pool

    International Nuclear Information System (INIS)

    Duffey, R.B.; Hughes, E.D.

    1991-01-01

    We consider the case of vapor condensation in a liquid pool, when the heat transfer is controlled by heat losses through the walls. The analysis is based on drift flux theory for phase separation in the pool, and determines the two-phase mixture height for the pool. To our knowledge this is the first analytical treatment of this classic problem that gives an explicit result, previous work having established the result for the evaporative case. From conservation of mass and energy in a one-dimensional steady flow, together with a void relation between the liquid and vapor fluxes, we determine the increase in the mixture level from the base level of the pool. It can be seen that the thermal and hydrodynamic influences are separable. Thus, the thermal influence of the wall heat transfer appears through its effect on the condensing length L*, so that at high condensation rates the pool is all liquid, and at low rates overflows (the level swell or foaming effect). Similarly, the phase separation effect hydrodynamically determines the height via the relative velocity of the mixture to the entering flux. We examine some practical applications of this result to level swell in condensing flows, and also examine some limits in ideal cases

  13. 13 CFR 120.1706 - Pool Originator's retained interest in Pool.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Pool Originator's retained interest in Pool. 120.1706 Section 120.1706 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan...

  14. Strategies for chemically healthy public swimming pools

    DEFF Research Database (Denmark)

    Hansen, Kamilla Marie Speht

    spreading of pathogens between swimmers because of its residual disinfection effect. In addition to potential contamination of pathogenic microorganisms, swimming pool water is polluted by organic matter deposited from the bathers such as saliva, urine, sweat, hair and personal care products. Since chlorine...... is a strong oxidant it oxidizes the organic matter in the pool water and forms disinfection byproducts (DBPs). More than 100 different DBPs have been identified. Some of these have been found to be genotoxic and may pose an increased cancer risk for the bathers. The aim of this thesis was to give an overview...... of the strategies which can be used to achieve microbiological safe water with low levels of DBPs to ensure healthy environment for bathers. There are different approaches to achieve healthy environment in public swimming pools which in this thesis are divided into three strategies: alternatives to chlorination...

  15. Simulation of pool scrubbing experiments using BUSCA

    International Nuclear Information System (INIS)

    Dehbi, A.; Guentay, S.

    1994-01-01

    BUSCA-PSI is a computer code which predicts the aerosol scrubbing taking place when gas bubbles containing fission products rise through stagnant pools of water after a postulated severe accident. A Lagrangian formulation is adopted to follow the path of a bubble as it rises toward the surface of the pool. The BUSCA model includes most aerosol removal mechanisms which are thought to be significant, namely: Jet Impaction at tile orifice, Convection/Diffusiophoresis during steam condensation, Thermophoresis, Sedimentation, Centrifugal Impaction during bubble rise, and Brownian Diffusion. The hydraulic modelling offers a variety of options for the initial globule volume, the stable bubble size, tile bubble rise velocity, and the bubble shape. The heat and mass transfer part of tile model uses correlations found in the relevant literature. BUSCA simulations were performed to determine the decontamination factor (DF) dependence on key aerosol and thermal hydraulic parameters. The decontamination factor increases with height, pool temperature subcooling, and steam content. The decontamination factor exhibits a parabolic dependence on the particle radius. At low particle sizes, the DF is high due to Brownian Diffusion which is the dominant removal mechanism. The DF hits a minimum and then increases with particle size as Centrifugal Impaction and Sedimentation become important. In separate calculations, BUSCA was used to the simulate the aerosol scrubbing experiments performed by EPRI. For cold pool tests, the predicted scrubbing efficiencies were in a good, conservative agreement with the data for both Tin and CsI, and the discrepancies were within the reported measurement errors. For hot pool tests, the code systematically underpredicted the scrubbing DF's; this is potentially due to condensation in the gas space above the pool, a situation not currently modelled by BUSCA. The code was also tested against data produced by the Tepco-Toshiba-Hitachi experiments. The

  16. Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments

    KAUST Repository

    Adel, Mustafa

    2016-09-06

    The central rift of the Red Sea has 25 brine pools with different physical and geochemical characteristics. Atlantis II (ATIID), Discovery Deeps (DD) and Chain Deep (CD) are characterized by high salinity, temperature and metal content. Several studies reported microbial communities in these brine pools, but few studies addressed the brine pool sediments. Therefore, sediment cores were collected from ATIID, DD, CD brine pools and an adjacent brine-influenced site. Sixteen different lithologic sediment sections were subjected to shotgun DNA pyrosequencing to generate 1.47 billion base pairs (1.47 × 109 bp). We generated sediment-specific reads and attempted to annotate all reads. We report the phylogenetic and biochemical uniqueness of the deepest ATIID sulfur-rich brine pool sediments. In contrary to all other sediment sections, bacteria dominate the deepest ATIID sulfur-rich brine pool sediments. This decrease in virus-to-bacteria ratio in selected sections and depth coincided with an overrepresentation of mobile genetic elements. Skewing in the composition of viruses-to-mobile genetic elements may uniquely contribute to the distinct microbial consortium in sediments in proximity to hydrothermally active vents of the Red Sea and possibly in their surroundings, through differential horizontal gene transfer.

  17. Applications of pooled DNA samples to the assessment of population affinities: short tandem repeats.

    Science.gov (United States)

    Crawford, M H; Banerjee, P; Demarchi, D A; Zlojutro, M; McComb, J; Livshits, G; Henneberg, M; Mosher, M J; Schanfield, M S; Knowles, J A

    2005-12-01

    Pooled DNA samples have been used in association studies of Mendelian disease genes. This method involves combining equal quantities of DNA from patients and control subjects into separate pools and comparing the pools for distributions of genetic markers. In this study identical quantities of DNA from 300 individuals representing 6 populations were pooled and amplified for 296 loci using the touchdown polymerase chain reaction (PCR) method. The purpose of this study is to test the efficacy of pooled DNA markers in the reconstruction of the genetic structure of human populations. The populations sampled included Chuvash, Buryats, Kizhi, Native Americans, South Africans, and New York City whites. To test the accuracy of the allele-frequency distributions, we genotyped the Buryats and New York samples individually for six microsatellite markers and compared their frequencies to the allele frequencies derived from the electropherogram peak heights for the pooled DNA, producing a correlation of 0.9811 with a variance of less than 0.04. Two-dimensional scaling of genetic distances among the six populations produced clusters that reflected known historical relationships. A distance matrix was created using all 296 loci, and matrices based on individual chromosomes were correlated against the total matrix. As expected, the largest chromosomes had the highest correlations with the total matrix, whereas one of the smallest chromosomes, chromosome 22, had the lowest correlation and differed most from the combined STR distance matrix.

  18. Zooplankton at deep Red Sea brine pools

    KAUST Repository

    Kaartvedt, Stein

    2016-03-02

    The deep-sea anoxic brines of the Red Sea comprise unique, complex and extreme habitats. These environments are too harsh for metazoans, while the brine–seawater interface harbors dense microbial populations. We investigated the adjacent pelagic fauna at two brine pools using net tows, video records from a remotely operated vehicle and submerged echosounders. Waters just above the brine pool of Atlantis II Deep (2000 m depth) appeared depleted of macrofauna. In contrast, the fauna appeared to be enriched at the Kebrit Deep brine–seawater interface (1466 m).

  19. One component, volume heated, boiling pool thermohydraulics

    International Nuclear Information System (INIS)

    Bede, M.; Perret, C.; Pretrel, H.; Seiler, J.M.

    1993-01-01

    Prior work on boiling pools provided heat exchange correlations valid for bubbly flow with laminar or turbulent boundary layers. New experiments performed with water (SEBULON) and UO 2 (SCARABEE BF2) in a churn-turbulent flow configuration show unexpected heat flux distributions for which the maximum heat flux may be situated well below the pool surface. The origin of this behaviour is attributed to condensation effects, very unstable boundary layer flow and surface oscillation. A calculation model is discussed which permits to approach the experimental heat flux distribution with reasonable accuracy. (authors). 7 figs., 2 appendix., 14 refs

  20. Numerical modeling of sodium fire – Part II: Pool combustion and combined spray and pool combustion

    International Nuclear Information System (INIS)

    Sathiah, Pratap; Roelofs, Ferry

    2014-01-01

    Highlights: • A CFD based method is proposed for the simulation of sodium pool combustion. • A sodium evaporation based model is proposed to model sodium pool evaporation. • The proposed method is validated against sodium pool experiments of Newman and Payne. • The results obtained using the proposed method are in good agreement with the experiments. - Abstract: The risk of sodium-air reaction has received considerable attention after the sodium-fire accident in Monju reactor. The fires resulting from the sodium-air reaction can be detrimental to the safety of a sodium fast reactor. Therefore, predicting the consequences of a sodium fire is important from a safety point of view. A computational method based on CFD is proposed here to simulate sodium pool fire and understand its characteristics. The method solves the Favre-averaged Navier-Stokes equation and uses a non-premixed mixture fraction based combustion model. The mass transfer of sodium vapor from the pool surface to the flame is obtained using a sodium evaporation model. The proposed method is then validated against well-known sodium pool experiments of Newman and Payne. The flame temperature and location predicted by the model are in good agreement with experiments. Furthermore, the trends of the mean burning rate with initial pool temperature and oxygen concentration are captured well. Additionally, parametric studies have been performed to understand the effects of pool diameter and initial air temperature on the mean burning rate. Furthermore, the sodium spray and sodium pool combustion models are combined to simulate simultaneous spray and pool combustion. Simulations were performed to demonstrate that the combined code could be applied to simulate this. Once sufficiently validated, the present code can be used for safety evaluation of a sodium fast reactor

  1. Numerical modeling of sodium fire – Part II: Pool combustion and combined spray and pool combustion

    Energy Technology Data Exchange (ETDEWEB)

    Sathiah, Pratap, E-mail: pratap.sathiah78@gmail.com [Shell Global Solutions Ltd., Brabazon House, Concord Business Park, Threapwood Road, Manchester M220RR (United Kingdom); Roelofs, Ferry, E-mail: roelofs@nrg.eu [Nuclear Research and Consultancy Group (NRG), Westerduinweg 3, 1755ZG Petten (Netherlands)

    2014-10-15

    Highlights: • A CFD based method is proposed for the simulation of sodium pool combustion. • A sodium evaporation based model is proposed to model sodium pool evaporation. • The proposed method is validated against sodium pool experiments of Newman and Payne. • The results obtained using the proposed method are in good agreement with the experiments. - Abstract: The risk of sodium-air reaction has received considerable attention after the sodium-fire accident in Monju reactor. The fires resulting from the sodium-air reaction can be detrimental to the safety of a sodium fast reactor. Therefore, predicting the consequences of a sodium fire is important from a safety point of view. A computational method based on CFD is proposed here to simulate sodium pool fire and understand its characteristics. The method solves the Favre-averaged Navier-Stokes equation and uses a non-premixed mixture fraction based combustion model. The mass transfer of sodium vapor from the pool surface to the flame is obtained using a sodium evaporation model. The proposed method is then validated against well-known sodium pool experiments of Newman and Payne. The flame temperature and location predicted by the model are in good agreement with experiments. Furthermore, the trends of the mean burning rate with initial pool temperature and oxygen concentration are captured well. Additionally, parametric studies have been performed to understand the effects of pool diameter and initial air temperature on the mean burning rate. Furthermore, the sodium spray and sodium pool combustion models are combined to simulate simultaneous spray and pool combustion. Simulations were performed to demonstrate that the combined code could be applied to simulate this. Once sufficiently validated, the present code can be used for safety evaluation of a sodium fast reactor.

  2. 13 CFR 120.1705 - Pool formation requirements.

    Science.gov (United States)

    2010-01-01

    ... requirements. SBA may adjust the Pool characteristics periodically based on program experience and market... a Pool involving a Pool Loan it does not own, it must purchase the Loan Interest it proposes to pool... purchase the Loan Interest and take it into inventory or settle the purchase of the Loan Interest through...

  3. Delineating probabilistic species pools in ecology and biogeography

    OpenAIRE

    Karger, Dirk Nikolaus; Cord, Anna F; Kessler, Michael; Kreft, Holger; Kühn, Ingolf; Pompe, Sven; Sandel, Brody; Sarmento Cabral, Juliano; Smith, Adam B; Svenning, Jens-Christian; Tuomisto, Hanna; Weigelt, Patrick; Wesche, Karsten

    2016-01-01

    Aim To provide a mechanistic and probabilistic framework for defining the species pool based on species-specific probabilities of dispersal, environmental suitability and biotic interactions within a specific temporal extent, and to show how probabilistic species pools can help disentangle the geographical structure of different community assembly processes. Innovation Probabilistic species pools provide an improved species pool definition based on probabilities in conjuncti...

  4. A strategy for optimizing item-pool management

    NARCIS (Netherlands)

    Ariel, A.; van der Linden, Willem J.; Veldkamp, Bernard P.

    2006-01-01

    Item-pool management requires a balancing act between the input of new items into the pool and the output of tests assembled from it. A strategy for optimizing item-pool management is presented that is based on the idea of a periodic update of an optimal blueprint for the item pool to tune item

  5. Multiplexed resequencing analysis to identify rare variants in pooled DNA with barcode indexing using next-generation sequencer.

    Science.gov (United States)

    Mitsui, Jun; Fukuda, Yoko; Azuma, Kyo; Tozaki, Hirokazu; Ishiura, Hiroyuki; Takahashi, Yuji; Goto, Jun; Tsuji, Shoji

    2010-07-01

    We have recently found that multiple rare variants of the glucocerebrosidase gene (GBA) confer a robust risk for Parkinson disease, supporting the 'common disease-multiple rare variants' hypothesis. To develop an efficient method of identifying rare variants in a large number of samples, we applied multiplexed resequencing using a next-generation sequencer to identification of rare variants of GBA. Sixteen sets of pooled DNAs from six pooled DNA samples were prepared. Each set of pooled DNAs was subjected to polymerase chain reaction to amplify the target gene (GBA) covering 6.5 kb, pooled into one tube with barcode indexing, and then subjected to extensive sequence analysis using the SOLiD System. Individual samples were also subjected to direct nucleotide sequence analysis. With the optimization of data processing, we were able to extract all the variants from 96 samples with acceptable rates of false-positive single-nucleotide variants.

  6. Public Swimming Pools | Florida Department of Health

    Science.gov (United States)

    Illness Disease Reporting and Surveillance Bureau of Public Health Laboratories Environmental Health Air Air Monitoring Carbon Monoxide Indoor Air Quality Mold Radon Water Aquatic Toxins Beach Water Quality purification, testing, treatment, and disinfection procedures. To ensure that the pool technicians are

  7. 28 CFR 540.64 - Press pools.

    Science.gov (United States)

    2010-07-01

    ... Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT CONTACT WITH PERSONS IN THE COMMUNITY Contact With News Media § 540.64 Press pools. (a) The Warden may establish a... shall notify all news media representatives who have requested interviews or visits that have not been...

  8. Stein's neuronal model with pooled renewal input

    Czech Academy of Sciences Publication Activity Database

    Rajdl, K.; Lánský, Petr

    2015-01-01

    Roč. 109, č. 3 (2015), s. 389-399 ISSN 0340-1200 Institutional support: RVO:67985823 Keywords : Stein’s model * Poisson process * pooled renewal processes * first-passage time Subject RIV: BA - General Mathematics Impact factor: 1.611, year: 2015

  9. Phase analysis in gated blood pool tomography

    International Nuclear Information System (INIS)

    Nakajima, Kenichi; Bunko, Hisashi; Tada, Akira; Taki, Junichi; Nanbu, Ichiro

    1984-01-01

    Phase analysis of gated blood pool study has been applied to detect the site of accessory conduction pathway (ACP) in the Wolff-Parkinson-White (WPW) syndrome; however, there was a limitation to detect the precise location of ACP by phase analysis alone. In this study, we applied phase analysis to gated blood pool tomography using seven pin hole tomography (7PT) and gated emission computed tomography (GECT) in 21 patients with WPW syndrome and 3 normal subjects. In 17 patients, the sites of ACPs were confirmed by epicardial mapping and the result of the surgical division of ACP. In 7PT, the site of ACP grossly agreed to the abnormal initial phase in phase image in 5 out of 6 patients with left cardiac type. In GECT, phase images were generated in short axial, vertical and horizontal long axial sections. In 8 out of 9 patients, the site of ACP was correctly identified by phase images, and in a patient who had two ACPs, initial phase corresponded to one of the two locations. Phase analysis of gated blood pool tomography has advantages for avoiding overlap of blood pools and for estimating three-dimensional propagation of the contraction, and can be a good adjunctive method in patients with WPW syndrome. (author)

  10. Swimming-pool piles; Piles piscines

    Energy Technology Data Exchange (ETDEWEB)

    Trioulaire, M [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1959-07-01

    In France two swimming-pool piles, Melusine and Triton, have just been set in operation. The swimming-pool pile is the ideal research tool for neutron fluxes of the order of 10{sup 13}. This type of pile can be of immediate interest to many research centres, but its cost must be reduced and a break with tradition should be observed in its design. It would be an advantage: - to bury the swimming-pool; - to reject the experimental channel; - to concentrate the cooling circuit in the swimming-pool; - to carry out all manipulations in the water; - to double the core. (author) [French] En France, deux piles piscines, Melusine et Triton, viennent d'entrer en service. La pile piscine est l'outil de recherche ideal pour des flux de neutrons de l'ordre de 10{sup 13}. Ce type de pile peut interesser des maintenant de nombreux centres de recherches mais il faut reduire son prix de revient et rompre avec le conformisme de sa conception. Il y a avantage: - a enterrer la piscine; - a supprimer les canaux experimentaux; - a concentrer le circuit de refrigeration dans la piscine; - a effectuer toutes les manipulations dans l'eau; - a doubler le coeur. (auteur)

  11. Spectrum pooling in MnWave Networks

    DEFF Research Database (Denmark)

    Boccardi, Federico; Shokri-Ghadikolaei, Hossein; Fodor, Gabor

    2016-01-01

    Motivated by the specific characteristics of mmWave technologies, we discuss the possibility of an authorization regime that allows spectrum sharing between multiple operators, also referred to as spectrum pooling. In particular, considering user rate as the performance measure, we assess...

  12. Pooling Objects for Recognizing Scenes without Examples

    NARCIS (Netherlands)

    Kordumova, S.; Mensink, T.; Snoek, C.G.M.

    2016-01-01

    In this paper we aim to recognize scenes in images without using any scene images as training data. Different from attribute based approaches, we do not carefully select the training classes to match the unseen scene classes. Instead, we propose a pooling over ten thousand of off-the-shelf object

  13. The new pooled cohort equations risk calculator

    DEFF Research Database (Denmark)

    Preiss, David; Kristensen, Søren L

    2015-01-01

    disease and any measure of social deprivation. An early criticism of the Pooled Cohort Equations Risk Calculator has been its alleged overestimation of ASCVD risk which, if confirmed in the general population, is likely to result in statin therapy being prescribed to many individuals at lower risk than...

  14. DNA pooling strategies for categorical (ordinal) traits

    Science.gov (United States)

    Despite reduced genotyping costs in recent years, obtaining genotypes for all individuals in a population may still not be feasible when sample size is large. DNA pooling provides a useful alternative to determining genotype effects. Clustering algorithms allow for grouping of individuals (observati...

  15. Pooling ASR data for closely related languages

    CSIR Research Space (South Africa)

    Van Heerden, C

    2010-05-01

    Full Text Available We describe several experiments that were conducted to assess the viability of data pooling as a means to improve speech-recognition performance for under-resourced languages. Two groups of closely related languages from the Southern Bantu language...

  16. "Teisele poole" Agambeniga ja Agambenita / Ragne Nukk

    Index Scriptorium Estoniae

    Nukk, Ragne, 1984-

    2012-01-01

    Tallinna Fotokuu rahvusvahelise fotonäituse "Teisele poole" ideeliste lähtekohtade tõlgendamisest. Autor kõrvutab Adam Budaki kuraatornäituse aluseks olnud Giorgio Agambeni esseed „Viimne kohtupäev“ näitusel eksponeeritud piltidega

  17. 7 CFR 1033.7 - Pool plant.

    Science.gov (United States)

    2010-01-01

    ... association and it fulfills the following conditions: (1) The aggregate monthly quantity supplied by all... or aseptically-processed fluid milk products. (c) A supply plant from which the quantity of bulk... operator of a supply plant located within the marketing area may include deliveries to pool distributing...

  18. Benthic assemblages of rock pools in northern Portugal: seasonal and between-pool variability

    Directory of Open Access Journals (Sweden)

    Iacopo Bertocci

    2012-11-01

    Full Text Available We investigated the seasonal (winter vs summer and within season and spatial (between-pool variability of benthic assemblages of rock pools at mid-intertidal level along the shore of Viana do Castelo (North Portugal. Physical traits of rock pools, including size, depth and position along the shore, were also compared between pools. While pools did not differ for any of the examined physical traits, results indicated a clear seasonal difference in the structure of assemblages, including a total of 49 macroalgal and 13 animal taxa. This finding was driven by six taxa that are more abundant in winter (the reef-forming polychaete Sabellaria alveolata, the articulated coralline algae Corallina spp., the brown alga Bifurcaria bifurcata, the encrusting coralline alga Lithophyllum incrustans, the red alga Chondracanthus acicularis and the grazing snails Gibbula spp. and four algal taxa that are more abundant in summer (the invasive brown Sargassum muticum, the green Ulva spp., the kelp Laminaria ochroleuca and the filamentous red Ceramium spp.. These data provide a new contribution to the knowledge of rock pool systems and have potential implications for monitoring programmes aimed at assessing ecological modifications related to natural and anthropogenic disturbances and for identifying processes responsible for the variability of rock pool assemblages.

  19. Identifying Genetic Signatures of Natural Selection Using Pooled Population Sequencing in Picea abies.

    Science.gov (United States)

    Chen, Jun; Källman, Thomas; Ma, Xiao-Fei; Zaina, Giusi; Morgante, Michele; Lascoux, Martin

    2016-07-07

    The joint inference of selection and past demography remain a costly and demanding task. We used next generation sequencing of two pools of 48 Norway spruce mother trees, one corresponding to the Fennoscandian domain, and the other to the Alpine domain, to assess nucleotide polymorphism at 88 nuclear genes. These genes are candidate genes for phenological traits, and most belong to the photoperiod pathway. Estimates of population genetic summary statistics from the pooled data are similar to previous estimates, suggesting that pooled sequencing is reliable. The nonsynonymous SNPs tended to have both lower frequency differences and lower FST values between the two domains than silent ones. These results suggest the presence of purifying selection. The divergence between the two domains based on synonymous changes was around 5 million yr, a time similar to a recent phylogenetic estimate of 6 million yr, but much larger than earlier estimates based on isozymes. Two approaches, one of them novel and that considers both FST and difference in allele frequencies between the two domains, were used to identify SNPs potentially under diversifying selection. SNPs from around 20 genes were detected, including genes previously identified as main target for selection, such as PaPRR3 and PaGI. Copyright © 2016 Chen et al.

  20. Generalizing Pooling Functions in CNNs: Mixed, Gated, and Tree.

    Science.gov (United States)

    Lee, Chen-Yu; Gallagher, Patrick; Tu, Zhuowen

    2018-04-01

    In this paper, we seek to improve deep neural networks by generalizing the pooling operations that play a central role in the current architectures. We pursue a careful exploration of approaches to allow pooling to learn and to adapt to complex and variable patterns. The two primary directions lie in: (1) learning a pooling function via (two strategies of) combining of max and average pooling, and (2) learning a pooling function in the form of a tree-structured fusion of pooling filters that are themselves learned. In our experiments every generalized pooling operation we explore improves performance when used in place of average or max pooling. We experimentally demonstrate that the proposed pooling operations provide a boost in invariance properties relative to conventional pooling and set the state of the art on several widely adopted benchmark datasets. These benefits come with only a light increase in computational overhead during training (ranging from additional 5 to 15 percent in time complexity) and a very modest increase in the number of model parameters (e.g., additional 1, 9, and 27 parameters for mixed, gated, and 2-level tree pooling operators, respectively). To gain more insights about our proposed pooling methods, we also visualize the learned pooling masks and the embeddings of the internal feature responses for different pooling operations. Our proposed pooling operations are easy to implement and can be applied within various deep neural network architectures.

  1. Corium quench in deep pool mixing experiments

    International Nuclear Information System (INIS)

    Spencer, B.W.; McUmber, L.; Gregorash, D.; Aeschlimann, R.; Sienicki, J.J.

    1985-01-01

    The results of two recent corium-water thermal interaction (CWTI) tests are described in which a stream of molten corium was poured into a deep pool of water in order to determine the mixing behavior, the corium-to-water heat transfer rates, and the characteristic sizes of the quenched debris. The corium composition was 60% UO 2 , 16% ZrO 2 , and 24% stainless steel by weight; its initial temperature was 3080 K, approx.160 K above the oxide phase liquidus temperature. The corium pour stream was a single-phase 2.2 cm dia liquid column which entered the water pool in film boiling at approx.4 m/s. The water subcooling was 6 and 75C in the two tests. Test results showed that with low subcooling, rapid steam generation caused the pool to boil up into a high void fraction regime. In contrast, with large subcooling no net steam generation occurred, and the pool remained relatively quiescent. Breakup of the jet appeared to occur by surface stripping. In neither test was the breakup complete during transit through the 32 cm deep water pool, and molten corium channeled to the base where it formed a melt layer. The characteristic heat transfer rates measured 3.5 MJ/s and 2.7 MJ/s during the fall stage for small and large subcooling, respectively; during the initial stage of bed quench, the surface heat fluxes measured 2.4 MW/m 2 and 3.7 MW/m 2 , respectively. A small mass of particles was formed in each test, measuring typically 0.1 to 1 mm and 1 to 5 mm dia for the large and small subcooling conditions, respectively. 9 refs., 13 figs., 1 tab

  2. Suncatcher and cool pool. Project report

    Energy Technology Data Exchange (ETDEWEB)

    Hammond, J.

    1981-03-01

    The Suncatcher is a simple, conical solar concentrating device that captures light entering clerestory windows and directs it onto thermal storage elements at the back of a south facing living space. The cone shape and inclination are designed to capture low angle winter sunlight and to reflect away higher angle summer sunlight. It is found that winter radiation through a Suncatcher window is 40 to 50% higher than through an ordinary window, and that the average solar fraction is 59%. Water-filled steal culvert pipes used for thermal storage are found to undergo less stratification, and thus to be more effective, when located where sunlight strikes the bottom rather than the top. Five Suncatcher buildings are described. Designs are considered for 32/sup 0/, 40/sup 0/ and 48/sup 0/ north latitude, and as the latitude increases, the inclination angle of the cone should be lowered. The Cool Pool is an evaporating, shaded roof pond which thermosiphons cool water into water-filled columns within a building. Preliminary experiments indicate that the best shade design has unimpeded north sky view, good ventilation, complete summer shading, a low architectural profile, and low cost attic vent lowers work. Another series of experiments established the satisfactory performance of the Cool Pool on a test building using four water-filled cylinders, two cylinders, and two cylinders connected to the Cool Pool through a heat exchanger. Although an unshaded pool cools better at night than a shaded one, daytime heat gain far offsets this advantage. A vinyl waterbag heat exchanger was developed for use with the Cool Pool. (LEW)

  3. Gene expression in colorectal cancer

    DEFF Research Database (Denmark)

    Birkenkamp-Demtroder, Karin; Christensen, Lise Lotte; Olesen, Sanne Harder

    2002-01-01

    Understanding molecular alterations in colorectal cancer (CRC) is needed to define new biomarkers and treatment targets. We used oligonucleotide microarrays to monitor gene expression of about 6,800 known genes and 35,000 expressed sequence tags (ESTs) on five pools (four to six samples in each...... pool) of total RNA from left-sided sporadic colorectal carcinomas. We compared normal tissue to carcinoma tissue from Dukes' stages A-D (noninvasive to distant metastasis) and identified 908 known genes and 4,155 ESTs that changed remarkably from normal to tumor tissue. Based on intensive filtering 226...

  4. Sampling and Pooling Methods for Capturing Herd Level Antibiotic Resistance in Swine Feces using qPCR and CFU Approaches

    DEFF Research Database (Denmark)

    Schmidt, Gunilla Veslemøy; Mellerup, Anders; Christiansen, Lasse Engbo

    2015-01-01

    The aim of this article was to define the sampling level and method combination that captures antibiotic resistance at pig herd level utilizing qPCR antibiotic resistance gene quantification and culture-based quantification of antibiotic resistant coliform indicator bacteria. Fourteen qPCR assays...... for commonly detected antibiotic resistance genes were developed, and used to quantify antibiotic resistance genes in total DNA from swine fecal samples that were obtained using different sampling and pooling methods. In parallel, the number of antibiotic resistant coliform indicator bacteria was determined...... when comparing individual sampling and pooling methods. qPCR on pooled samples was found to be a good representative for the general resistance level in a pig herd compared to the coliform CFU counts. It had significantly reduced relative standard deviations compared to coliform CFU counts in the same...

  5. Horizontal above-rack pool storage

    International Nuclear Information System (INIS)

    Moscardini, R.L.

    1993-08-01

    This report describes a unique method for storing spent, six year out of core, fuel at a prototypical PWR nuclear power station. The study describes a conceptual design, with favorable structural, thermal and criticality technical evaluations. However, economic considerations and licensing risks are judged to be less favorable. The concept study prescribes a fuel over fuel arrangement in an existing Spent Fuel Pool (SFP) with full maintenance of ALARA principles. This concept study is specific to a prototypical pool design, but may easily be projected to other nuclear facilities with other SFP conditions. For the prototypical PWR, the conceptual fuel bridge design will store over 200 additional fuel assemblies without significant facility modifications and for an indefinite time period

  6. Sodium pool fire model for CONACS code

    International Nuclear Information System (INIS)

    Yung, S.C.

    1982-01-01

    The modeling of sodium pool fires constitutes an important ingredient in conducting LMFBR accident analysis. Such modeling capability has recently come under scrutiny at Westinghouse Hanford Company (WHC) within the context of developing CONACS, the Containment Analysis Code System. One of the efforts in the CONACS program is to model various combustion processes anticipated to occur during postulated accident paths. This effort includes the selection or modification of an existing model and development of a new model if it clearly contributes to the program purpose. As part of this effort, a new sodium pool fire model has been developed that is directed at removing some of the deficiencies in the existing models, such as SOFIRE-II and FEUNA

  7. Pool Boiling CHF in Inclined Narrow Annuli

    International Nuclear Information System (INIS)

    Kang, Myeong Gie

    2010-01-01

    Pool boiling heat transfer has been studied extensively since it is frequently encountered in various heat transfer equipment. Recently, it has been widely investigated in nuclear power plants for application to the advanced light water reactors designs. Through the review on the published results it can be concluded that knowledge on the combined effects of the surface orientation and a confined space on pool boiling heat transfer is of great practical importance and also of great academic interest. Fujita et al. investigated pool boiling heat transfer, from boiling inception to the critical heat flux (CHF, q' CHF ), in a confined narrow space between heated and unheated parallel rectangular plates. They identified that both the confined space and the surface orientation changed heat transfer much. Kim and Suh changed the surface orientation angles of a downward heating rectangular channel having a narrow gap from the downward-facing position (180 .deg.) to the vertical position (90 .deg.). They observed that the CHF generally decreased as the inclination angle (θ ) increased. Yao and Chang studied pool boiling heat transfer in a confined heat transfer for vertical narrow annuli with closed bottoms. They observed that when the gap size ( s ) of the annulus was decreased the effect of space confinement to boiling heat transfer increased. The CHF was occurred at much lower value for the confined space comparing to the unconfined pool boiling. Pool boiling heat transfer in narrow horizontal annular crevices was studied by Hung and Yao. They concluded that the CHF decreased with decreasing gap size of the annuli and described the importance of the thin film evaporation to explain the lower CHF of narrow crevices. The effect of the inclination angle on the CHF on countercurrent boiling in an inclined uniformly heated tube with closed bottoms was also studied by Liu et al. They concluded that the CHF reduced with the inclination angle decrease. A study was carried out

  8. Review and assessment of pool scrubbing models

    International Nuclear Information System (INIS)

    Herranz, L.E.; Escudero, M.J.; Peyres, V.; Polo, J.; Lopez-Jimenez, J.

    1996-01-01

    Decontamination of fission products bearing bubbles as they through aqueous pools becomes a crucial phenomenon for source term evaluation of hypothetical risk dominant sequences of Light Water Reactors. In the present report a peer review and assessment of models encapsulated in SPARC andBUSCA codes is presented. Several aspects of pool scrubbing have been addressed: particle removal, fission product vapour retention and bubble hydrodynamics. Particular emphasis has been given to the close link between retention and hydrodynamics, from both modelling and experimental point of view. In addition, RHR and SGTR sequences were simulated with SPARC90 and BUSCA-AUG92 codes, and their results were compared with those obtained with MAAP 3.0B.As a result of this work, model capabilities and shortcomings have beenassessed and some areas susceptible of further research have been identified.(Author) 73 refs

  9. Review and assessment of pool scrubbing models

    International Nuclear Information System (INIS)

    Herranz, L.E.; Escudero, M.J.; Peyres, V.; Polo, J.; Lopez, J.

    1996-01-01

    Decontamination of fission products bearing bubbles as they pass through aqueous pools becomes a crucial phenomenon for source term evaluation of hypothetical risk dominant sequences of Light Water Reactors. In the present report a peer review and assessment of models encapsulated in SPARC and BUSCA codes is presented. Several aspects of pool scrubbing have been addressed: particle removal, fission product vapour retention and bubble hydrodynamics. Particular emphasis has been given to the close link between retention and hydrodynamics, from both modelling and experimental point of view. In addition, RHR and SGTR sequences were simulated with SPARC90 and BUSCA-AUG92 codes, and their results were compared with those obtained with MAAP 3.0B. As a result of this work, model capabilities and shortcomings have been assessed and some areas susceptible of further research have been identified. (Author) 73 refs

  10. Review and assessment of pool scrubbing models

    Energy Technology Data Exchange (ETDEWEB)

    Herranz, L.E.; Escudero, M.J.; Peyres, V.; Polo, J.; Lopez, J.

    1996-07-01

    Decontamination of fission products bearing bubbles as they pass through aqueous pools becomes a crucial phenomenon for source term evaluation of hypothetical risk dominant sequences of Light Water Reactors. In the present report a peer review and assessment of models encapsulated in SPARC and BUSCA codes is presented. Several aspects of pool scrubbing have been addressed: particle removal, fission product vapour retention and bubble hydrodynamics. Particular emphasis has been given to the close link between retention and hydrodynamics, from both modelling and experimental point of view. In addition, RHR and SGTR sequences were simulated with SPARC90 and BUSCA-AUG92 codes, and their results were compared with those obtained with MAAP 3.0B. As a result of this work, model capabilities and shortcomings have been assessed and some areas susceptible of further research have been identified. (Author) 73 refs.

  11. Pricing Electricity in Pools With Wind Producers

    DEFF Research Database (Denmark)

    Morales González, Juan Miguel; Conejo, A. J.; Kai Liu

    2012-01-01

    This paper considers an electricity pool that includes a significant number of wind producers and is cleared through a network-constrained auction, one day in advance and on an hourly basis. The hourly auction is formulated as a two-stage stochastic programming problem, where the first stage...... represents the clearing of the market and the second stage models the system operation under a number of plausible wind production realizations. This formulation co-optimizes energy and reserve, and allows deriving both pool energy prices and balancing energy prices. These prices result in both cost recovery...... for producers and revenue reconciliation. A case study of realistic size is used to illustrate the functioning of the proposed pricing scheme....

  12. Application of pooled genotyping to scan candidate regions for association with HDL cholesterol levels

    Directory of Open Access Journals (Sweden)

    Hinds David A

    2004-11-01

    Full Text Available Abstract Association studies are used to identify genetic determinants of complex human traits of medical interest. With the large number of validated single nucleotide polymorphisms (SNPs currently available, two limiting factors in association studies are genotyping capability and costs. Pooled DNA genotyping has been proposed as an efficient means of screening SNPs for allele frequency differences in case-control studies and for prioritising them for subsequent individual genotyping analysis. Here, we apply quantitative pooled genotyping followed by individual genotyping and replication to identify associations with human serum high-density lipoprotein (HDL cholesterol levels. The DNA from individuals with low and high HDL cholesterol levels was pooled separately, each pool was amplified by polymerase chain reaction in triplicate and each amplified product was separately hybridised to a high-density oligonucleotide array. Allele frequency differences between case and control groups with low and high HDL cholesterol levels were estimated for 7,283 SNPs distributed across 71 candidate gene regions spanning a total of 17.1 megabases. A novel method was developed to take advantage of independently derived haplotype map information to improve the pooled estimates of allele frequency differences. A subset of SNPs with the largest estimated allele frequency differences between low and high HDL cholesterol groups was chosen for individual genotyping in the study population, as well as in a separate replication population. Four SNPs in a single haplotype block within the cholesteryl ester transfer protein (CETP gene interval were significantly associated with HDL cholesterol levels in both populations. Our study is among the first to demonstrate the application of pooled genotyping followed by confirmation with individual genotyping to identify genetic determinants of a complex trait.

  13. Pool type liquid metal fast breeder reactors

    International Nuclear Information System (INIS)

    Guthrie, B.M.

    1978-08-01

    Various technical aspects of the liquid metal fast breeder reactor (LMFBR), specifically pool type LMFBR's, are summarized. The information presented, for the most part, draws upon existing data. Special sections are devoted to design, technical feasibility (normal operating conditions), and safety (accident conditions). A survey of world fast reactors is presented in tabular form, as are two sets of reference reactor parameters based on available data from present and conceptual LMFBR's. (auth)

  14. Condensation of vapor bubble in subcooled pool

    Science.gov (United States)

    Horiuchi, K.; Koiwa, Y.; Kaneko, T.; Ueno, I.

    2017-02-01

    We focus on condensation process of vapor bubble exposed to a pooled liquid of subcooled conditions. Two different geometries are employed in the present research; one is the evaporation on the heated surface, that is, subcooled pool boiling, and the other the injection of vapor into the subcooled pool. The test fluid is water, and all series of the experiments are conducted under the atmospheric pressure condition. The degree of subcooling is ranged from 10 to 40 K. Through the boiling experiment, unique phenomenon known as microbubble emission boiling (MEB) is introduced; this phenomenon realizes heat flux about 10 times higher than the critical heat flux. Condensation of the vapor bubble is the key phenomenon to supply ambient cold liquid to the heated surface. In order to understand the condensing process in the MEB, we prepare vapor in the vapor generator instead of the evaporation on the heated surface, and inject the vapor to expose the vapor bubble to the subcooled liquid. Special attention is paid to the dynamics of the vapor bubble detected by the high-speed video camera, and on the enhancement of the heat transfer due to the variation of interface area driven by the condensation.

  15. Biomass production and energy source of thermophiles in a Japanese alkaline geothermal pool.

    Science.gov (United States)

    Kimura, Hiroyuki; Mori, Kousuke; Nashimoto, Hiroaki; Hattori, Shohei; Yamada, Keita; Koba, Keisuke; Yoshida, Naohiro; Kato, Kenji

    2010-02-01

    Microbial biomass production has been measured to investigate the contribution of planktonic bacteria to fluxations in dissolved organic matter in marine and freshwater environments, but little is known about biomass production of thermophiles inhabiting geothermal and hydrothermal regions. The biomass production of thermophiles inhabiting an 85 degrees C geothermal pool was measured by in situ cultivation using diffusion chambers. The thermophiles' growth rates ranged from 0.43 to 0.82 day(-1), similar to those of planktonic bacteria in marine and freshwater habitats. Biomass production was estimated based on cellular carbon content measured directly from the thermophiles inhabiting the geothermal pool, which ranged from 5.0 to 6.1 microg C l(-1) h(-1). This production was 2-75 times higher than that of planktonic bacteria in other habitats, because the cellular carbon content of the thermophiles was much higher. Quantitative PCR and phylogenetic analysis targeting 16S rRNA genes revealed that thermophilic H2-oxidizing bacteria closely related to Calderobacterium and Geothermobacterium were dominant in the geothermal pool. Chemical analysis showed the presence of H2 in gases bubbling from the bottom of the geothermal pool. These results strongly suggested that H2 plays an important role as a primary energy source of thermophiles in the geothermal pool.

  16. Extra-binomial variation approach for analysis of pooled DNA sequencing data

    Science.gov (United States)

    Wallace, Chris

    2012-01-01

    Motivation: The invention of next-generation sequencing technology has made it possible to study the rare variants that are more likely to pinpoint causal disease genes. To make such experiments financially viable, DNA samples from several subjects are often pooled before sequencing. This induces large between-pool variation which, together with other sources of experimental error, creates over-dispersed data. Statistical analysis of pooled sequencing data needs to appropriately model this additional variance to avoid inflating the false-positive rate. Results: We propose a new statistical method based on an extra-binomial model to address the over-dispersion and apply it to pooled case-control data. We demonstrate that our model provides a better fit to the data than either a standard binomial model or a traditional extra-binomial model proposed by Williams and can analyse both rare and common variants with lower or more variable pool depths compared to the other methods. Availability: Package ‘extraBinomial’ is on http://cran.r-project.org/ Contact: chris.wallace@cimr.cam.ac.uk Supplementary information: Supplementary data are available at Bioinformatics Online. PMID:22976083

  17. An accurate clone-based haplotyping method by overlapping pool sequencing.

    Science.gov (United States)

    Li, Cheng; Cao, Changchang; Tu, Jing; Sun, Xiao

    2016-07-08

    Chromosome-long haplotyping of human genomes is important to identify genetic variants with differing gene expression, in human evolution studies, clinical diagnosis, and other biological and medical fields. Although several methods have realized haplotyping based on sequencing technologies or population statistics, accuracy and cost are factors that prohibit their wide use. Borrowing ideas from group testing theories, we proposed a clone-based haplotyping method by overlapping pool sequencing. The clones from a single individual were pooled combinatorially and then sequenced. According to the distinct pooling pattern for each clone in the overlapping pool sequencing, alleles for the recovered variants could be assigned to their original clones precisely. Subsequently, the clone sequences could be reconstructed by linking these alleles accordingly and assembling them into haplotypes with high accuracy. To verify the utility of our method, we constructed 130 110 clones in silico for the individual NA12878 and simulated the pooling and sequencing process. Ultimately, 99.9% of variants on chromosome 1 that were covered by clones from both parental chromosomes were recovered correctly, and 112 haplotype contigs were assembled with an N50 length of 3.4 Mb and no switch errors. A comparison with current clone-based haplotyping methods indicated our method was more accurate. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  18. POOL WATER TREATMENT AND COOLING SYSTEM DESCRIPTION DOCUMENT

    International Nuclear Information System (INIS)

    King, V.

    2000-01-01

    The Pool Water Treatment and Cooling System is located in the Waste Handling Building (WHB), and is comprised of various process subsystems designed to support waste handling operations. This system maintains the pool water temperature within an acceptable range, maintains water quality standards that support remote underwater operations and prevent corrosion, detects leakage from the pool liner, provides the capability to remove debris from the pool, controls the pool water level, and helps limit radiological exposure to personnel. The pool structure and liner, pool lighting, and the fuel staging racks in the pool are not within the scope of the Pool Water Treatment and Cooling System. Pool water temperature control is accomplished by circulating the pool water through heat exchangers. Adequate circulation and mixing of the pool water is provided to prevent localized thermal hotspots in the pool. Treatment of the pool water is accomplished by a water treatment system that circulates the pool water through filters, and ion exchange units. These water treatment units remove radioactive and non-radioactive particulate and dissolved solids from the water, thereby providing the water clarity needed to conduct waste handling operations. The system also controls pool water chemistry to prevent advanced corrosion of the pool liner, pool components, and fuel assemblies. Removal of radioactivity from the pool water contributes to the project ALARA (as low as is reasonably achievable) goals. A leak detection system is provided to detect and alarm leaks through the pool liner. The pool level control system monitors the water level to ensure that the minimum water level required for adequate radiological shielding is maintained. Through interface with a demineralized water system, adequate makeup is provided to compensate for loss of water inventory through evaporation and waste handling operations. Interface with the Site Radiological Monitoring System provides continuous

  19. Infections Unlikely to be Spread Through Swimming Pools

    Science.gov (United States)

    ... Water Home Infections Unlikely to be Spread Through Swimming Pools Language: English (US) Español (Spanish) Recommend on ... included below. Infections Unlikely to be Spread by Swimming Pools Head Lice Head lice are unlikely to ...

  20. Thermal analyses of solar swimming pool heating in Pakistan

    International Nuclear Information System (INIS)

    Ahmad, I.

    2011-01-01

    Hotels and swimming clubs in Pakistan pay huge gas bills for heating Swimming pools in winter. Winter days in most parts of Pakistan remain sunny and unglazed low cost solar collectors may be used to extend the swimming season. Installing the pool in a wind-protected area, which receives unobstructed solar radiation, may further reduce the size of the solar collectors required to heat the swimming pools. The pools should be covered with plastic sheet to eliminate evaporative heat losses and to prevent dust and tree leaves falling in the pool. The results of the thermal analysis show that in some parts of the country, a solar exposed pool can maintain comfortable temperature simply by using a plastic sheet on the pool surface. On the other hand, there are cities where solar collector array equal to twice the surface area of the pool is required to keep desired temperature in winter. (author)

  1. Analysis of sodium pool fire in SFEF for assessing the limiting pool fire

    International Nuclear Information System (INIS)

    Mangarjuna Rao, P.; Ramesh, S.S.; Nashine, B.K.; Kasinathan, N.; Chellapandi, P.

    2011-01-01

    Accidental sodium leaks and resultant sodium fires in Liquid Metal Fast Breeder Reactor (LMFBR) systems can create a threat to the safe operation of the plant. To avoid this defence-in depth approach is implemented from the design stage of reactor itself. Rapid detection of sodium leak and fast dumping of the sodium into the storage tank of a defective circuit, leak collection trays, adequate lining of load bearing structural concrete and extinguishment of the sodium fire are the important defensive measures in the design, construction and operation of a LMFBR for protection against sodium leaks and their resultant fires. Evaluation of sodium leak events and their consequences by conducting large scale engineering experiments is very essential for effective implementation of the above protection measures for sodium fire safety. For this purpose a Sodium Fire Experimental Facility (SFEF) is constructed at SED, IGCAR. SFEF is having an experimental hall of size 9 m x 6 m x 10 m with 540 m 3 volume and its design pressure is 50 kPa. It is a concrete structure and provided with SS 304 liner, which is fixed to the inside surfaces of walls, ceiling and floor. A leak tight door of size (1.8 m x 2.0 m) is provided to the experimental hall and the facility is provided with a sodium equipment hall and a control room. Experimental evaluation of sodium pool fire consequences is an important activity in the LMFBR sodium fire safety related studies. An experimental program has been planned for different types of sodium fire studies in SFEF. A prior to that numerical analysis have been carried out for enclosed sodium pool fires using SOFIRE-II sodium pool fire code for SFEF experimental hall configuration to evaluate the limiting pool fire. This paper brings out results of the analysis carried out for this purpose. Limiting pool fire of SFEF depends on the exposed surface area of the pool, amount of sodium in the pool, oxygen concentration and initial sodium temperature. Limiting

  2. (Important hygienic aspects for swimming pools (author's transl))

    Energy Technology Data Exchange (ETDEWEB)

    Somosi, G

    1981-01-01

    The major epidemics which occurred in Hungary and originated from water in swimming pools are reported. The difficulties encountered in producing epidemiological evidence and in monitoring infections originating from water in swimming pools are mentioned. The possibilities of controlling the water quality in swimming pools and of preventing infections are discussed. Reference is made to the existing bacteriological limit values in Hungary to be observed in the recirculation of water in swimming pools.

  3. Aspects of DNA repair and nucleotide pool imbalance

    Energy Technology Data Exchange (ETDEWEB)

    Holliday, R.

    1985-01-01

    Evidence that optimum repair depends on adequate pools of deoxynucleotide triphosphates (dNTPs) comes from the study of pyrimidine auxotrophs of Ustilago maydis. These strains are sensitive to UV light and X-rays, and for pyr1-1 it has been shown that the intracellular concentration of dTTP is reduced about 7-fold. The survival curve of pyr1-1 after UV-treatment, and split dose experiments with wild-type cells, provide evidence for an inducible repair mechanism, which probably depends on genetic recombination. Although inducible repair saves cellular resources, it has the disadvantage of becoming ineffective at doses which are high enough to inactivate the repressed structural gene(s) for repair enzymes. It is clear that a wide variety of repair mechanisms have evolved to remove lesions which arise either spontaneously or as a result of damage from external agents. Nevertheless, it would be incorrect to assume that all species require all possible pathways of repair. It is now well established that the accuracy of DNA and protein synthesis depends on proof-reading or editing mechanisms. Optimum accuracy levels will evolve from the balance between error avoidance in macromolecular synthesis and physiological efficiency in growth and propagation.

  4. NACUBO's Guide to Unitizing Investment Pools. Second Edition

    Science.gov (United States)

    Wheeler, Mary S.

    2011-01-01

    The National Association of College and University Business Officers' (NACUBO's) "Guide to Unitizing Investment Pools" addresses the principles and concepts for administering a consolidated investment pool. Unitization is the mechanism by which investment funds are pooled to maximize investment efficiencies and provide information for donors,…

  5. A skeleton for distributed work pools in Eden

    DEFF Research Database (Denmark)

    Dieterle, Mischa; Berthold, Jost; Loogen, Rita

    2010-01-01

    We present a flexible skeleton for implementing distributed work pools in our parallel functional language Eden. The skeleton manages a pool of tasks (work pool) in a distributed manner using a demand-driven work stealing approach for load balancing. All coordination is done locally within...

  6. Opportunities and challenges when pooling milk samples using ELISA

    DEFF Research Database (Denmark)

    Græsbøll, Kaare; Andresen, Lars Ole; Hisham Beshara Halasa, Tariq

    2017-01-01

    -positive samples by pooling. To illustrate this, the sensitivity of antibody ELISA on pooled samples of bovine milk for Salmonella Dublin, Mycobacterium avium spp. paratuberculosis, and bovine virus diarrhea was tested. For these milk assays, the analytical sensitivity decreased rapidly with increasing pool sizes...

  7. 17 CFR 4.22 - Reporting to pool participants.

    Science.gov (United States)

    2010-04-01

    ..., the financial statements are not required to include consolidated information for all series. (7) For... event that the International Financial Reporting Standards require consolidated financial statements for... reporting pool's consolidated financial statements. (ii) The commodity pool operator of a pool that meets...

  8. 48 CFR 873.114 - Best value pool.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Best value pool. 873.114... SUPPLEMENTARY REGULATIONS SIMPLIFIED ACQUISITION PROCEDURES FOR HEALTH-CARE RESOURCES 873.114 Best value pool... solicitation. These vendors constitute the best value pool. This determination is within the sole discretion of...

  9. Measurements in large JP-4 pool fires

    International Nuclear Information System (INIS)

    Keltner, N.R.; Kent, L.A.; Schneider, M.E.

    1987-01-01

    Over the past four years, Sandia National Laboratories has conducted a number of large pool fire tests to evaluate the design of radioactive material (RAM) shipping containers. Some of these tests have been designed to define the thermal environment and some have been used for certification testing. In each test there have been a number of fire diagnostic measurements. The simplest sets of diagnostics have involved measurements of temperature at several elevations on arrays of towers, measurements of hot wall heat flux with small calorimeters suspended from the towers, the average fuel recession rate, and the wind speed and direction. The most complex sets of diagnostics have included the above and in various tests added radiometers in the lower flame zone, centerline velocity measurements at a number of elevations, radiometers and calorimeters at the fuel surface, large cylindrical and flat plate calorimeters, infrared imaging, time resolved fuel recession rates, and a variety of soot particle concentration and size measurements made in the plume with a tethered balloon and an instrumented airplane. All of the large fires have been conducted in a 9.1 m by 18.3 m pool using JP-4 as the fuel. Typical duration is one-half hour. Covering all of the results is beyond the scope of a single paper. Conditionally sampled temperature and velocity measurements from one fire will be presented; for this fire, a 20 cm layer of fuel was floated on 61 cm of water. Pool surface heat flux, fuel recession rate data, and smoke emission data from a second fire are given. Because the wind has a strong effect on the temperature and velocity measurements, conditional sampling has been used to try to obtain data during periods of low winds. 10 refs., 3 figs

  10. 454 sequencing of pooled BAC clones on chromosome 3H of barley

    Directory of Open Access Journals (Sweden)

    Yamaji Nami

    2011-05-01

    Full Text Available Abstract Background Genome sequencing of barley has been delayed due to its large genome size (ca. 5,000Mbp. Among the fast sequencing systems, 454 liquid phase pyrosequencing provides the longest reads and is the most promising method for BAC clones. Here we report the results of pooled sequencing of BAC clones selected with ESTs genetically mapped to chromosome 3H. Results We sequenced pooled barley BAC clones using a 454 parallel genome sequencer. A PCR screening system based on primer sets derived from genetically mapped ESTs on chromosome 3H was used for clone selection in a BAC library developed from cultivar "Haruna Nijo". The DNA samples of 10 or 20 BAC clones were pooled and used for shotgun library development. The homology between contig sequences generated in each pooled library and mapped EST sequences was studied. The number of contigs assigned on chromosome 3H was 372. Their lengths ranged from 1,230 bp to 58,322 bp with an average 14,891 bp. Of these contigs, 240 showed homology and colinearity with the genome sequence of rice chromosome 1. A contig annotation browser supplemented with query search by unique sequence or genetic map position was developed. The identified contigs can be annotated with barley cDNAs and reference sequences on the browser. Homology analysis of these contigs with rice genes indicated that 1,239 rice genes can be assigned to barley contigs by the simple comparison of sequence lengths in both species. Of these genes, 492 are assigned to rice chromosome 1. Conclusions We demonstrate the efficiency of sequencing gene rich regions from barley chromosome 3H, with special reference to syntenic relationships with rice chromosome 1.

  11. Gated cardiac blood pool studies in arrhythmias

    International Nuclear Information System (INIS)

    Itti, R.; Casset, D.; Philippe, L.; Cosnay, P.; Fauchier, J.P.

    1988-01-01

    Biventricular phase analysis a gated blood pool studies may help to solve two fundamental questions raised by patients suffering from arrhythmias: localization of an electrical cardiac activation abnormality by means of contraction mapping and assesment of an underlying organic disease using the phase histograms and their standard deviations. Three groups of patients have been evaluated to demonstrate the usefulness of radioisotopic techniques in arrhythmias: 36 patients with a Wolff-Parkinson-White syndrom, 27 patients studied during a ventricular tachycardia attack and 32 patients suspected of arrhythmogenic ventricular dysplasia. Correlations with invasive electrophysiologic studies are presented and the diagnostic and therapeutic implications of these results are discussed [fr

  12. Pool boiling visualization on open microchannel surfaces

    Directory of Open Access Journals (Sweden)

    Kaniowski Robert

    2017-01-01

    Full Text Available The paper presents visualization investigations into pool boiling heat transfer for open minichannel surfaces. The experiments were carried out wih saturated water at atmospheric pressure. Parallel microchannels fabricated by machining were about 0.3 mm wide and 0.2 to 0.4 mm deep. High-speed videos were used as an aid to understanding the heat transfer mechanism. The visualization study aimed at identifying nucleation sites of the departing bubbles and determining their diameters and frequency at various superheats.

  13. Simulation of a pool type research reactor

    International Nuclear Information System (INIS)

    Oliveira, Andre Felipe da Silva de; Moreira, Maria de Lourdes

    2011-01-01

    Computational fluid dynamic is used to simulate natural circulation condition after a research reactor shutdown. A benchmark problem was used to test the viability of usage such code to simulate the reactor model. A model which contains the core, the pool, the reflector tank, the circulation pipes and chimney was simulated. The reactor core contained in the full scale model was represented by a porous media. The parameters of porous media were obtained from a separate CFD analysis of the full core model. Results demonstrate that such studies can be carried out for research and test of reactors design. (author)

  14. Gated cardiac blood pool studies in arrhythmias

    Energy Technology Data Exchange (ETDEWEB)

    Itti, R.; Casset, D.; Philippe, L.; Cosnay, P.; Fauchier, J.P.

    1988-01-01

    Biventricular phase analysis a gated blood pool studies may help to solve two fundamental questions raised by patients suffering from arrhythmias: localization of an electrical cardiac activation abnormality by means of contraction mapping and assesment of an underlying organic disease using the phase histograms and their standard deviations. Three groups of patients have been evaluated to demonstrate the usefulness of radioisotopic techniques in arrhythmias: 36 patients with a Wolff-Parkinson-White syndrom, 27 patients studied during a ventricular tachycardia attack and 32 patients suspected of arrhythmogenic ventricular dysplasia. Correlations with invasive electrophysiologic studies are presented and the diagnostic and therapeutic implications of these results are discussed.

  15. Pool film boiling heat transfer, 5

    International Nuclear Information System (INIS)

    Sakurai, A.; Shiotsu, M.; Hata, K.

    1981-01-01

    Steady minimum film boiling heat flux and temperature were experimentally studied for a horizontal cylinder test heater in a pool of saturated water under pressures ranging from 0.1 to 2 MPa. Minimum temperature of film boiling may be determined by hydrodynamic Taylor instability for the pressures lower than around 1.0 MPa and by homogeneous nucleation temperature for the higher pressures. However, conventional correlations of minimum heat flux based on the hydrodynamic Taylor instability cannot at all predict the pressure dependency of the experimental data in the lower pressure region. Semi-empirical equation of the minimum heat flux based on the hydrodynamic Taylor instability was given. (author)

  16. Swimming pool reactor reliability and safety analysis

    International Nuclear Information System (INIS)

    Li Zhaohuan

    1997-01-01

    A reliability and safety analysis of Swimming Pool Reactor in China Institute of Atomic Energy is done by use of event/fault tree technique. The paper briefly describes the analysis model, analysis code and main results. Meanwhile it also describes the impact of unassigned operation status on safety, the estimation of effectiveness of defense tactics in maintenance against common cause failure, the effectiveness of recovering actions on the system reliability, the comparison of occurrence frequencies of the core damage by use of generic and specific data

  17. Genes and Gene Therapy

    Science.gov (United States)

    ... correctly, a child can have a genetic disorder. Gene therapy is an experimental technique that uses genes to ... or prevent disease. The most common form of gene therapy involves inserting a normal gene to replace an ...

  18. Chinese nuclear insurance and Chinese nuclear insurance pool

    International Nuclear Information System (INIS)

    Gong Zhiqi

    2000-01-01

    Chinese Nuclear Insurance Started with Daya Bay Nuclear Power Station, PICC issued the insurance policy. Nuclear insurance cooperation between Chinese and international pool's organizations was set up in 1989. In 1996, the Chinese Nuclear Insurance Pool was prepared. The Chinese Nuclear Insurance Pool was approved by The Chinese Insurance Regulatory Committee in May of 1999. The principal aim is to centralize maximum the insurance capacity for nuclear insurance from local individual insurers and to strengthen the reinsurance relations with international insurance pools so as to provide the high quality insurance service for Chinese nuclear industry. The Member Company of Chinese Nuclear Pool and its roles are introduced in this article

  19. A method of measuring a molten metal liquid pool volume

    Science.gov (United States)

    Garcia, G.V.; Carlson, N.M., Donaldson, A.D.

    1990-12-12

    A method of measuring a molten metal liquid pool volume and in particular molten titanium liquid pools, including the steps of (a) generating an ultrasonic wave at the surface of the molten metal liquid pool, (b) shining a light on the surface of a molten metal liquid pool, (c) detecting a change in the frequency of light, (d) detecting an ultrasonic wave echo at the surface of the molten metal liquid pool, and (e) computing the volume of the molten metal liquid. 3 figs.

  20. Study of mixed convection in sodium pool

    International Nuclear Information System (INIS)

    Wang Zhou; Chen Yan

    1995-01-01

    The mixed convection phenomena in the sodium pool of fast reactor have been studied systematically by the two dimensional modeling method. A generalized concept of circumferential line in the cylindrical coordinates was proposed to overcome the three dimensional effect induced by the pool geometry in an analysis of two dimensional modeling. A method of sub-step in time was developed for solving the turbulent equations. The treatments on the boundary condition for the auxiliary velocity field have been proposed, and the explanation of allowing the flow function method to be used in the flow field in presence of a mass source term was given. As examples of verification, the experiments were conducted with water flow in a rectangular cavity. The results from theoretical analysis were applied to the numerical computation for the mixed convection in the cavity. The mechanism of stratified flow in the cavity was studied. A numerical calculation was carried out for the mixed convection in hot plenum of a typical fast reactor

  1. Visual attention and flexible normalization pools

    Science.gov (United States)

    Schwartz, Odelia; Coen-Cagli, Ruben

    2013-01-01

    Attention to a spatial location or feature in a visual scene can modulate the responses of cortical neurons and affect perceptual biases in illusions. We add attention to a cortical model of spatial context based on a well-founded account of natural scene statistics. The cortical model amounts to a generalized form of divisive normalization, in which the surround is in the normalization pool of the center target only if they are considered statistically dependent. Here we propose that attention influences this computation by accentuating the neural unit activations at the attended location, and that the amount of attentional influence of the surround on the center thus depends on whether center and surround are deemed in the same normalization pool. The resulting form of model extends a recent divisive normalization model of attention (Reynolds & Heeger, 2009). We simulate cortical surround orientation experiments with attention and show that the flexible model is suitable for capturing additional data and makes nontrivial testable predictions. PMID:23345413

  2. Hardening Stratum, the Bitcoin Pool Mining Protocol

    Directory of Open Access Journals (Sweden)

    Recabarren Ruben

    2017-07-01

    Full Text Available Stratum, the de-facto mining communication protocol used by blockchain based cryptocurrency systems, enables miners to reliably and efficiently fetch jobs from mining pool servers. In this paper we exploit Stratum’s lack of encryption to develop passive and active attacks on Bitcoin’s mining protocol, with important implications on the privacy, security and even safety of mining equipment owners. We introduce StraTap and ISP Log attacks, that infer miner earnings if given access to miner communications, or even their logs. We develop BiteCoin, an active attack that hijacks shares submitted by miners, and their associated payouts. We build BiteCoin on WireGhost, a tool we developed to hijack and surreptitiously maintain Stratum connections. Our attacks reveal that securing Stratum through pervasive encryption is not only undesirable (due to large overheads, but also ineffective: an adversary can predict miner earnings even when given access to only packet timestamps. Instead, we devise Bedrock, a minimalistic Stratum extension that protects the privacy and security of mining participants. We introduce and leverage the mining cookie concept, a secret that each miner shares with the pool and includes in its puzzle computations, and that prevents attackers from reconstructing or hijacking the puzzles.

  3. Estimated environmental radionuclide transfer and deposition into outdoor swimming pools

    International Nuclear Information System (INIS)

    Tagami, Kazumi; Nagata, Izumi; Sueki, Keisuke

    2014-01-01

    In 2011, a large radioactive discharge occurred at the Fukushima Daiichi nuclear power plant. This plant is located within a climatically temperate region where outdoor swimming pools are popular. Although it is relatively easy to decontaminate pools by refilling them with fresh water, it is difficult to maintain safe conditions given highly contaminated diurnal dust falls from the surrounding contaminated ground. Our objectives in this paper were to conduct daily radioactivity measurements, to determine the quantity of radioactive contaminants from the surrounding environment that invade outdoor pools, and to investigate the efficacy of traditional pool cleaners in removing radioactive contaminants. The depositions in the paper filterable particulates ranged from 0 to 62,5 Bq/m 2 /day, with the highest levels found in the southern Tohoku District containing Fukushima Prefecture and in the Kanto District containing Tokyo Metro. They were approximately correlated with the ground contamination. Traditional pool cleaners eliminated 99% of contaminants at the bottom of the pool, reducing the concentration to 41 Bq/m 2 after cleaning. Authors recommended the deposition or the blown radionuclides into outdoor swimming pools must be considered into pool regulations when the environments exactly polluted with radionuclides. - Highlights: • Deposition into outdoor swimming pool in a habitable areas estimated 72 Bq/m 2 /day. • More than 500 Bq/m 2 /day deposition will exceed our national guideline (10 Bq/l) of swimming pool. • Vacuum pool cleaner eliminates 99% radionuclides deposition

  4. Plant diversity associated with pools in natural and restored peatlands

    Directory of Open Access Journals (Sweden)

    N. Fontaine

    2007-06-01

    Full Text Available This study describes plant assemblages associated with the edges of peatland pools. We conducted inventories in six natural peatlands in the province of Québec (Canada in order to measure the contribution of pools to species diversity in climatic regions where peatlands are used for peat extraction. We also carried out vegetation surveys in a peatland that has been restored after peat extraction/harvesting to determine whether pool vegetation establishes along the edges of created pools when dry surface restoration techniques only are used. Pools enhanced plant species richness in natural peatlands. Around created pools, species associated with natural pools were still absent, and non-bog species were present, six years after restoration. On this basis, we emphasise the importance of preserving natural peatlands with pools. In order to restore fully the plant diversity associated with peatlands at harvested sites, it may be necessary to modify pool excavation techniques so that created pools resemble more closely those in natural peatlands. Active introduction of the plant species or communities associated with natural pools may also be needed; candidate species for North America include Andromeda glaucophylla, Cladopodiella fluitans, Carex limosa, Eriophorum virginicum, Rhynchospora alba and Sphagnum cuspidatum.

  5. Life cycle environmental implications of residential swimming pools.

    Science.gov (United States)

    Forrest, Nigel; Williams, Eric

    2010-07-15

    Ownership of private swimming pools in the U.S. grew 2 to 4% per annum from 1997 to 2007. The environmental implications of pool ownership are analyzed by hybrid life cycle assessment (LCA) for nine U.S. cities. An operational model is constructed estimating consumption of chemicals, water, and energy for a typical residential pool. The model incorporates geographical climatic variations and upstream water and energy use from electricity and water supply networks. Results vary considerably by city: a factor of 5-6 for both water and energy use. Water use is driven by aridness and length of the swimming season, while energy use is mainly driven by length of the swimming season. Water and energy impacts of pools are significant, particularly in arid climates. In Phoenix for example pools account for 22% and 13% of a household's electricity and water use, respectively. Measures to reduce water and energy use in pools such as optimizing the pump schedule and covering the pool in winter can realize greater savings than many common household efficiency improvements. Private versus community pools are also compared. Community pools in Phoenix use 60% less swimming pool water and energy per household than subdivisions without community pools.

  6. Reduction of the pool-top radiation level in HANARO

    International Nuclear Information System (INIS)

    Lee, Choong-Sung; Park, Sang-Jun; Kim, Heonil; Park, Yong-Chul; Choi, Young-San

    1999-01-01

    HANARO is an open-tank-in-pool type reactor. Pool water is the only shielding to minimize the pool top radiation level. During the power ascension test of HANARO, the measured pool top radiation level was higher than the design value because some of the activation products in the coolant reached the pool surface. In order to suppress this rising coolant, the hot water layer system (HWL) was designed and installed to maintain l.2 meter-deep hot water layer whose temperature is 5degC higher than that of the underneath pool surface. After the installation of the HWL system, however, the radiation level of the pool-top did not satisfy the design value. The operation modes of the hot water layer system and the other systems in the reactor pool, which had an effect on the formation of the hot water layer, were changed to reduce pool-top radiation level. After the above efforts, the temperature and the radioactivity distribution in the pool was measured to confirm whether this system blocked the rising coolant. The radiation level at the pool-top was significantly reduced below one tenth of that before installing the HWL and satisfied the design value. It was also confirmed by calculation that this hot water layer system would significantly reduce the release of fission gases to the reactor hall and the environment during the hypothetical accident as well. (author)

  7. Design of inventory pools in spare part support operation systems

    Science.gov (United States)

    Mo, Daniel Y.; Tseng, Mitchell M.; Cheung, Raymond K.

    2014-06-01

    The objective of a spare part support operation is to fulfill the part request order with different service contracts in the agreed response time. With this objective to achieve different service targets for multiple service contracts and the considerations of inventory investment, it is not only important to determine the inventory policy but also to design the structure of inventory pools and the order fulfilment strategies. In this research, we focused on two types of inventory pools: multiple inventory pool (MIP) and consolidated inventory pool (CIP). The idea of MIP is to maintain separated inventory pools based on the types of service contract, while CIP solely maintains a single inventory pool regardless of service contract. Our research aims to design the inventory pool analytically and propose reserve strategies to manage the order fulfilment risks in CIP. Mathematical models and simulation experiments would be applied for analysis and evaluation.

  8. Behavior of spent nuclear fuel in water pool storage

    International Nuclear Information System (INIS)

    Johnson, A.B. Jr.

    1977-09-01

    Storage of irradiated nuclear fuel in water pools (basins) has been standard practice since nuclear reactors first began operation approximately 34 years ago. Pool storage is the starting point for all other fuel storage candidate processes and is a candidate for extended interim fuel storage until policy questions regarding reprocessing and ultimate disposal have been resolved. This report assesses the current performance of nuclear fuel in pool storage, the range of storage conditions, and the prospects for extending residence times. The assessment is based on visits to five U.S. and Canadian fuel storage sites, representing nine storage pools, and on discussions with operators of an additional 21 storage pools. Spent fuel storage experience from British pools at Winfrith and Windscale and from a German pool at Karlsruhe (WAK) also is summarized

  9. Investigation of the condition of spent-fuel pool components

    International Nuclear Information System (INIS)

    Kustas, F.M.; Bates, S.O.; Opitz, B.E.; Johnson, A.B. Jr.; Perez, J.M. Jr.; Farnsworth, R.K.

    1981-09-01

    It is currently projected that spent nuclear fuel, which is discharged from the reactor and then stored in water pools, may remain in those pools for several decades. Other studies have addressed the expected integrity of the spent fuel during extended water storage; this study assesses the integrity of metallic spent fuel pool components. Results from metallurgical examinations of specimens taken from stainless steel and aluminum components exposed in spent fuel pools are presented. Licensee Event Reports (LERs) relating to problems with spent fuel components were assessed and are summarized to define the types of operational problems that have occurred. The major conclusions of this study are: aluminum and stainless steel spent fuel pool components have a good history of performance in both deionized and borated water pools. Although some operational problems involving pool components have occurred, these problems have had minimal impacts

  10. Decontamination of outdoor school swimming pools in Fukushima

    International Nuclear Information System (INIS)

    Saegusa, Jun

    2013-01-01

    After the Fukushima Daiichi NPP accident following the Great East Japan Earthquake, many school swimming pools in Fukushima have suspended water discharge, due to concerns that pool water which contains radioactive fallout is discharged into a river or waterway for agricultural use. The Japan Atomic Energy Agency conducted researches and examinations on the existing absorbent method and the flocculation method as ways for decontaminating pool water. By reviewing and improving these methods through decontamination demonstrations at eight pools in Fukushima, a practical decontamination method for outdoor pools has been established. This report summarizes the methods and results of the decontamination demonstrations carried out at the schools. Also, the surface density of fallout estimated at one of the pools is also presented and discussed in connection with the overall collection ratio of radiocesium at the pool. (author)

  11. Investigation of the condition of spent-fuel pool components

    Energy Technology Data Exchange (ETDEWEB)

    Kustas, F.M.; Bates, S.O.; Opitz, B.E.; Johnson, A.B. Jr.; Perez, J.M. Jr.; Farnsworth, R.K.

    1981-09-01

    It is currently projected that spent nuclear fuel, which is discharged from the reactor and then stored in water pools, may remain in those pools for several decades. Other studies have addressed the expected integrity of the spent fuel during extended water storage; this study assesses the integrity of metallic spent fuel pool components. Results from metallurgical examinations of specimens taken from stainless steel and aluminum components exposed in spent fuel pools are presented. Licensee Event Reports (LERs) relating to problems with spent fuel components were assessed and are summarized to define the types of operational problems that have occurred. The major conclusions of this study are: aluminum and stainless steel spent fuel pool components have a good history of performance in both deionized and borated water pools. Although some operational problems involving pool components have occurred, these problems have had minimal impacts.

  12. Expanding the live kidney donor pool: ethical considerations regarding altruistic donors, paired and pooled programs.

    Science.gov (United States)

    Patel, Shaneel Rajendra; Chadha, Priyanka; Papalois, Vassilios

    2011-06-01

    In renal transplant, there is a well-known deficiency in organ supply relative to demand. Live donation provides superior results when compared with deceased donation including a better rate of graft success and fewer immunologic complications. This deficiency in organs leads to significant morbidity and mortality rates. Alternative avenues have been extensively explored that may expand the live donor pool. They include altruistic donation as well as paired and pooled exchange programs. Altruistic donation is a truly selfless act from a donor unknown to the recipient. Kidney paired donation involves 2 incompatible donor-recipient pairs swapping donors to produce compatibility. Pooled donation involves at least 2 pairs, and can take the form of domino chains in which altruistic input sets up a chain of transplants, in which each recipient's incompatible donor makes a donation for the next recipient. Despite application of these various methods, there lie extensive ethical issues surrounding them. Misconceptions frequently occur; for instance, the perceived benefit that donating an organ to a loved one is greater for a related donor than for an altruistic one. Additionally, it is frequently believed that immunologic incompatibility offers coerced donors liberation from surgery, and that overcoming these barriers by introducing exchange programs provides vulnerable donors less protection. This article explores these and other complex ethical issues surrounding the various methods of expanding the donor pool. The authors offer opinions that challenge the ethical issues and attempt to overcome those views that hinder progress in the field.

  13. Microbial contributions to coupled arsenic and sulfur cycling in the acid-sulfide hot spring Champagne Pool, New Zealand.

    Science.gov (United States)

    Hug, Katrin; Maher, William A; Stott, Matthew B; Krikowa, Frank; Foster, Simon; Moreau, John W

    2014-01-01

    Acid-sulfide hot springs are analogs of early Earth geothermal systems where microbial metal(loid) resistance likely first evolved. Arsenic is a metalloid enriched in the acid-sulfide hot spring Champagne Pool (Waiotapu, New Zealand). Arsenic speciation in Champagne Pool follows reaction paths not yet fully understood with respect to biotic contributions and coupling to biogeochemical sulfur cycling. Here we present quantitative arsenic speciation from Champagne Pool, finding arsenite dominant in the pool, rim and outflow channel (55-75% total arsenic), and dithio- and trithioarsenates ubiquitously present as 18-25% total arsenic. In the outflow channel, dimethylmonothioarsenate comprised ≤9% total arsenic, while on the outflow terrace thioarsenates were present at 55% total arsenic. We also quantified sulfide, thiosulfate, sulfate and elemental sulfur, finding sulfide and sulfate as major species in the pool and outflow terrace, respectively. Elemental sulfur concentration reached a maximum at the terrace. Phylogenetic analysis of 16S rRNA genes from metagenomic sequencing revealed the dominance of Sulfurihydrogenibium at all sites and an increased archaeal population at the rim and outflow channel. Several phylotypes were found closely related to known sulfur- and sulfide-oxidizers, as well as sulfur- and sulfate-reducers. Bioinformatic analysis revealed genes underpinning sulfur redox transformations, consistent with sulfur speciation data, and illustrating a microbial role in sulfur-dependent transformation of arsenite to thioarsenate. Metagenomic analysis also revealed genes encoding for arsenate reductase at all sites, reflecting the ubiquity of thioarsenate and a need for microbial arsenate resistance despite anoxic conditions. Absence of the arsenite oxidase gene, aio, at all sites suggests prioritization of arsenite detoxification over coupling to energy conservation. Finally, detection of methyl arsenic in the outflow channel, in conjunction with

  14. Technical specification for fabrication of HANARO pool cover

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, Jeong Soo; Woo, Sang Ik

    2001-06-01

    This technical specification details the requirements and the acceptance criteria for design, seismic analysis, function test, installation and quality assurance for HANARO pool cover which will be installed at the top of reactor pool. The pool cover is classified as non-nuclear safety, seismic category II and quality class T. The basic design of the pool cover for increasing HANARO applications has been carried out for supporting the driving devices which can load, unload and rotate the irradiation targets in the in-core and out-core vertical irradiation holes under on-power operation. The comments of HANARO user group related with irradiation tests have optimally considered in the process of design. The interference between fuel handling and control absorber units in the reactor pool and activities to load, unload and rotate the irradiation targets at the top of the reactor pool have been minimized. The pool cover can be moved for maintenance and can protect the reactor pool from unexpected drop of foreign materials. It provides the space to vertical access of driving devices for NTD, CT/IR and OR4/OR5 under on-power operation. And the pool cover assembly must maintain its structural integrity under seismic load. Based on the above design concept, the HANARO pool cover has been proposed as supporting structure of driving devices for NTD, fission moly and RI production under on-power operation.

  15. Technical Facilities Management, Loan Pool, and Calibration

    Science.gov (United States)

    Smith, Jacob

    2011-01-01

    My work at JPL for the SURF program began on June 11, 2012 with the Technical Facilities Management group (TFM). As well as TFM, I worked with Loan Pool and Metrology to help them out with various tasks. Unlike a lot of other interns, I did not have a specific project rather many different tasks to be completed over the course of the 10 weeks.The first task to be completed was to sort through old certification reports in 6 different boxes to locate reports that needed to be archived into a digital database. There were no reports within these boxes that needed to be archived but rather were to be shredded. The reports went back to the early 1980's and up to the early 2000's. I was looking for reports dated from 2002 to 2012

  16. Soil carbon pools in different pasture systems

    Directory of Open Access Journals (Sweden)

    Francisco M. Cardozo, Jr.

    2016-03-01

    Full Text Available The aim of this study was to assess the carbon pools of a tropical soil where the native forest was replaced with different pasture systems. We studied five pasture production systems, including four monoculture systems with forage grasses such as Andropogon, Brachiaria, Panicum, and Cynodon, and an agroforestry system as well as a native vegetation plot. Greater availability of fulvic acid was detected in the agroforestry system as compared with that in the other systems. Higher lability of C was detected in the Andropogon system during the dry and rainy seasons and during the dry season in Cynodon. During the dry season, all pastures systems showed deficits in the net removal of atmospheric CO2. The structure and practices of the agroforestry system enables more carbon to be sequestered in the soil as compared with the monoculture pasture, suggesting that it is an important practice to mitigate climatic change and to improve soil quality.

  17. Corrosion surveillance in spent fuel storage pools

    International Nuclear Information System (INIS)

    Howell, J.P.

    1996-01-01

    In mid-1991, corrosion of aluminum-clad spent nuclear fuel was observed in the light-water filled basins at the Savannah River site. A corrosion surveillance program was initiated in the P, K, L-Reactor basins and in the Receiving Basin for Offsite Fuels (RBOF). This program verified the aggressive nature of the pitting corrosion and provided recommendations for changes in basin operations to permit extended longer term interim storage. The changes were implemented during 1994--1996 and have resulted in significantly improved basin water quality with conductivity in the 1--3 microS/cm range. Under these improved conditions, no new pitting has been observed over the last three years. This paper describes the corrosion surveillance program at SRS and what has been learned about the corrosion of aluminum-clad in spent fuel storage pools

  18. Drop Impact on to Moving Liquid Pools

    Science.gov (United States)

    Muñoz-Sánchez, Beatriz Natividad; Castrejón-Pita, José Rafael; Castrejón-Pita, Alfonso Arturo; Hutchings, Ian M.

    2014-11-01

    The deposition of droplets on to moving liquid substrates is an omnipresent situation both in nature and industry. A diverse spectrum of phenomena emerges from this simple process. In this work we present a parametric experimental study that discerns the dynamics of the impact in terms of the physical properties of the fluid and the relative velocity between the impacting drop and the moving liquid pool. The behaviour ranges from smooth coalescence (characterized by little mixing) to violent splashing (generation of multiple satellite droplets and interfacial vorticity). In addition, transitional regimes such as bouncing and surfing are also found. We classify the system dynamics and show a parametric diagram for the conditions of each regime. This work was supported by the EPSRC (Grant EP/H018913/1), the Royal Society, Becas Santander Universidades and the International Relationships Office of the University of Extremadura.

  19. One knowledge base or many knowledge pools?

    DEFF Research Database (Denmark)

    Lundvall, Bengt-Åke

    It is increasingly realized that knowledge is the most important resource and that learning is the most important process in the economy. Sometimes this is expressed by coining the current era as characterised by a ‘knowledge based economy'. But this concept might be misleading by indicating...... that there is one common knowledge base on which economic activities can be built. In this paper we argue that it is more appropriate to see the economy as connecting to different ‘pools of knowledge'. The argument is built upon a conceptual framework where we make distinctions between private/public, local....../global, individual/collective and tacit/codified knowledge. The purpose is both ‘academic' and practical. Our analysis demonstrates the limits of a narrowly economic perspective on knowledge and we show that these distinctions have important implications both for innovation policy and for management of innovation....

  20. [Investigation of toxigenic microcystis and microcystin pollution in Huayuankou Conservation Pool of Yellow River].

    Science.gov (United States)

    Ban, Haiqun; Ba, Yue; Cheng, Xuemin; Wang, Guangzhou

    2007-09-01

    To investigate the contaminative, condition of planktonic algae, cyanobacteria, toxigenic microcystis and microcystin in Huayuankou Conservation Pool of Yellow River. From March 2005 to January 2006, water samples were taken 15 times by 2. 5L plastic sampler from Huayuankou Conservation Pool. The density of algae were counted by using blood cell counter. Phycocyanin intergenic spacer region (PC-IGS) and microcystin synthetase gene B (mcyB) of toxigenic microcystis was identified by the whole cell PCR. The concentration of microcystin was determined by ELISA kit. The positive results of PCR and ELISA were compared. Bacillariophyta, chlorophyta, cyanophyta (cyanobacteria) and euglenophyta were main algaes in Huayuankou conservation pool, and the dominant algae and cell density changed seasonally. Algae cell density and cyanobacteria cell density were higher in summer and autumn than in spring and winter. From July to November, 2005, PC-IGS and mcyB were detected positively by whole cell PCR. Microcystin was positively detected from July, the concentration of microcystin changed from 0 to 0.25microg/L, it was more higher in summer than other seasons. Toxigenic microcystis and microcystin could be detected in Huayuankou Conservation Pool of Yellow River. Whole cell PCR could be used to identify toxigenic microcystis.

  1. Genomic analyses of tropical beef cattle fertility based on genotyping pools of Brahman cows with unknown pedigree.

    Science.gov (United States)

    Reverter, A; Porto-Neto, L R; Fortes, M R S; McCulloch, R; Lyons, R E; Moore, S; Nicol, D; Henshall, J; Lehnert, S A

    2016-10-01

    We introduce an innovative approach to lowering the overall cost of obtaining genomic EBV (GEBV) and encourage their use in commercial extensive herds of Brahman beef cattle. In our approach, the DNA genotyping of cow herds from 2 independent properties was performed using a high-density bovine SNP chip on DNA from pooled blood samples, grouped according to the result of a pregnancy test following their first and second joining opportunities. For the DNA pooling strategy, 15 to 28 blood samples from the same phenotype and contemporary group were allocated to pools. Across the 2 properties, a total of 183 pools were created representing 4,164 cows. In addition, blood samples from 309 bulls from the same properties were also taken. After genotyping and quality control, 74,584 remaining SNP were used for analyses. Pools and individual DNA samples were related by means of a "hybrid" genomic relationship matrix. The pooled genotyping analysis of 2 large and independent commercial populations of tropical beef cattle was able to recover significant and plausible associations between SNP and pregnancy test outcome. We discuss 24 SNP with significant association ( < 1.0 × 10) and mapped within 40 kb of an annotated gene. We have established a method to estimate the GEBV in young herd bulls for a trait that is currently unable to be predicted at all. In summary, our novel approach allowed us to conduct genomic analyses of fertility in 2 large commercial Brahman herds managed under extensive pastoral conditions.

  2. Indoor swimming pools. Humidity caused problems and suggested solutions

    Energy Technology Data Exchange (ETDEWEB)

    1989-01-01

    Reports have been received from across Canada on premature deterioration and other problems of indoor swimming pool buildings. This technical paper has been prepared to assist pool managers to solve these problems, which typically include leaking roofs, condensation on inside walls, peeling paint, efflorescence, rusting of metal elements, deterioration of concrete block structures, and high costs for pool heating. An effective insulation and vapor barrier system for a swimming pool roof is described, and the high relative humidity of the typical pool building is discussed as the primary cause of most problems. Proper sealing to cut down air infiltration is recommended, along with proper maintenance and painting. High energy costs are often due to low insulation values and to excessive ventilation used for decreasing the humidity. By using dehumidifiers capable of heat recovery, and by placing an insulating blanket on the pool after operating hours, it is shown that substantial cost savings are possible. 10 figs.

  3. Turbulence model for melt pool natural convection heat transfer

    International Nuclear Information System (INIS)

    Kelkar, K.M.; Patankar, S.V.

    1994-01-01

    Under severe reactor accident scenarios, pools of molten core material may form in the reactor core or in the hemispherically shaped lower plenum of the reactor vessel. Such molten pools are internally heated due to the radioactive decay heat that gives rise to buoyant flows in the molten pool. The flow in such pools is strongly influenced by the turbulent mixing because the expected Rayleigh numbers under accidents scenarios are very high. The variation of the local heat flux over the boundaries of the molten pools are important in determining the subsequent melt progression behavior. This study reports results of an ongoing effort towards providing a well validated mathematical model for the prediction of buoyant flow and heat transfer in internally heated pool under conditions expected in severe accident scenarios

  4. Sharing as risk pooling in a social dilemma experiment

    Directory of Open Access Journals (Sweden)

    Todd L. Cherry

    2015-03-01

    Full Text Available In rural economies with missing or incomplete markets, idiosyncratic risk is frequently pooled through informal networks. Idiosyncratic shocks, however, are not limited to private goods but can also restrict an individual from partaking in or benefiting from a collective activity. In these situations, a group must decide whether to provide insurance to the affected member. We describe results of a laboratory experiment designed to test whether a simple sharing institution can sustain risk pooling in a social dilemma with idiosyncratic risk. We tested whether risk could be pooled without a commitment device and, separately, whether effective risk pooling induced greater cooperation in the social dilemma. We found that even in the absence of a commitment device or reputational considerations, subjects voluntarily pooled risk, thereby reducing variance in individual earnings. In spite of effective risk pooling, however, cooperation in the social dilemma was unaffected.

  5. A Pooled Genome-Wide Association Study of Asperger Syndrome.

    Directory of Open Access Journals (Sweden)

    Varun Warrier

    Full Text Available Asperger Syndrome (AS is a neurodevelopmental condition characterized by impairments in social interaction and communication, alongside the presence of unusually repetitive, restricted interests and stereotyped behaviour. Individuals with AS have no delay in cognitive and language development. It is a subset of Autism Spectrum Conditions (ASC, which are highly heritable and has a population prevalence of approximately 1%. Few studies have investigated the genetic basis of AS. To address this gap in the literature, we performed a genome-wide pooled DNA association study to identify candidate loci in 612 individuals (294 cases and 318 controls of Caucasian ancestry, using the Affymetrix GeneChip Human Mapping version 6.0 array. We identified 11 SNPs that had a p-value below 1x10-5. These SNPs were independently genotyped in the same sample. Three of the SNPs (rs1268055, rs7785891 and rs2782448 were nominally significant, though none remained significant after Bonferroni correction. Two of our top three SNPs (rs7785891 and rs2782448 lie in loci previously implicated in ASC. However, investigation of the three SNPs in the ASC genome-wide association dataset from the Psychiatric Genomics Consortium indicated that these three SNPs were not significantly associated with ASC. The effect sizes of the variants were modest, indicating that our study was not sufficiently powered to identify causal variants with precision.

  6. DNA Probe Pooling for Rapid Delineation of Chromosomal Breakpoints

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Chun-Mei; Kwan, Johnson; Baumgartner, Adolf; Weier, Jingly F.; Wang, Mei; Escudero, Tomas; Munne' , Santiago; Zitzelsberger, Horst F.; Weier, Heinz-Ulrich

    2009-01-30

    Structural chromosome aberrations are hallmarks of many human genetic diseases. The precise mapping of translocation breakpoints in tumors is important for identification of genes with altered levels of expression, prediction of tumor progression, therapy response, or length of disease-free survival as well as the preparation of probes for detection of tumor cells in peripheral blood. Similarly, in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for carriers of balanced, reciprocal translocations benefit from accurate breakpoint maps in the preparation of patient-specific DNA probes followed by a selection of normal or balanced oocytes or embryos. We expedited the process of breakpoint mapping and preparation of case-specific probes by utilizing physically mapped bacterial artificial chromosome (BAC) clones. Historically, breakpoint mapping is based on the definition of the smallest interval between proximal and distal probes. Thus, many of the DNA probes prepared for multi-clone and multi-color mapping experiments do not generate additional information. Our pooling protocol described here with examples from thyroid cancer research and PGD accelerates the delineation of translocation breakpoints without sacrificing resolution. The turnaround time from clone selection to mapping results using tumor or IVF patient samples can be as short as three to four days.

  7. The burning and smoke release rates of sodium pool fires

    International Nuclear Information System (INIS)

    Newman, R.N.; Payne, J.F.B.

    1976-10-01

    The burning rates and smoke release fractions of sodium pool fires have been measured over the pool temperature range 250 0 C to 750 0 C. A theoretical model is derived which satisfactorily predicts the burning rate over the above temperature range. The theory further predicts that the burning rate should be independent of pool diameter, a prediction supported by a comparison of burning rate data from this study and available data from other studies. (author)

  8. Pool scrubbing and hydrodynamic experiments on jet injection regime

    International Nuclear Information System (INIS)

    Peyres, V.; Espigares, M.M.; Polo, J.; Escudero, M.J.; Herranz, L.E.; Lopez-Jimenez, J.

    1995-01-01

    Plant analyses have shown that pool scrubbing can play an important role in source term during PWR risk dominant sequences. An examination ofboundary conditions governing fission products and aerosols transport through aqueous beds revealed that most of radioactivity is discharged into the pool under jet injection regime. This fact and the lack of experimental data under such conditions pointed the need of setting out an experimental programme which provided reliable experimental data to validate code models. In this report the major results of a pool scrubbing experimental programme carried out in PECA facility are presented. One of the major findings was that a remarkable fraction of particle absorption was not a function of the residence time of bubbles rising through the pool. Such a contribution was assumed to be associated to aerosol removal mechanisms acting at the pool entrance. As a consequence, a hydrodynamic experimental plan was launched to examine the gas behaviour during the initial stages in the pool. Size and shape of gas nuclei the pool were measured and fitted to a long normal distribution. Particularly, size was found to be quite sensitive to inletgas flow and at minor extent to gas composition and pool temperature. SPARC90 and BUSCA-AUG92 were used to simulate the retention tests. Whereas SPARC90 showed a pretty good agreement with experimental data, BUSCA-AUG92 results were far away from measurements in all the cases. SPARC90consistency apparently pointed out the important role of fission products and aerosols retention at the injection zone; nonetheless, a peer examination of pool scrubbing phenomenology at the pool entrance should be carried out to test both hydrodynamic and removal models. Hence, one of the major highlights drawn from this work was the need of further research under representative severe accident conditions (i.e., saturated pools, jet injection regimes, etc.), as well as separate effect tests to validate, improve and

  9. Reserve growth in oil pools of Alberta : model and forecast

    Energy Technology Data Exchange (ETDEWEB)

    Verma, M.; Cook, T. [United States Geological Survey, Denver, CO (United States). Central Region

    2010-09-15

    This paper presented a reserve growth study that was conducted on oil pools in Alberta, Canada. Historical oil reserve data were evaluated to assess the potential for future reserve growth in both pools and fields, and reserve growth models and functions were developed to better forecast hydrocarbon volumes. The study also considered the sensitivity of reserve growth to such factors as pool size, porosity, and oil gravity. From 1960 to 2005, the reported known recoverable oil in Alberta, excluding the Athabasca oil sands and including only pools with adequate data, increased from 4.2 to 13.9 billion barrels of oil (BBO). New discoveries contributed 3.7 BBO and reserve growth added 6 BBO. Most reserve growth occurred in pools with more than 125,000 barrels of oil. Light-oil pools account for most of the total known oil volume and consequently showed the lowest growth. Pools with greater than 30 percent porosity grew more than pools with lower porosity reservoirs. Oil field growth was found to be almost twice that of pool growth, possibly because the analysis evaluated fields with two or more pools discovered in different years. The growth in oil volumes in Alberta pools is projected to be about 454 million barrels of oil in the period from 2006 to 2010. Over a 25-year period, the cumulative reserve growth in Alberta oil pools was substantially lower than other major petroleum-producing regions, but the growth at the field level compares well. 8 refs., 2 tabs., 9 figs.

  10. Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools

    Directory of Open Access Journals (Sweden)

    Thomson Pippa

    2003-09-01

    Full Text Available Abstract In a project on the biodiversity of chickens funded by the European Commission (EC, eight laboratories collaborated to assess the genetic variation within and between 52 populations from a wide range of chicken types. Twenty-two di-nucleotide microsatellite markers were used to genotype DNA pools of 50 birds from each population. The polymorphism measures for the average, the least polymorphic population (inbred C line and the most polymorphic population (Gallus gallus spadiceus were, respectively, as follows: number of alleles per locus, per population: 3.5, 1.3 and 5.2; average gene diversity across markers: 0.47, 0.05 and 0.64; and proportion of polymorphic markers: 0.91, 0.25 and 1.0. These were in good agreement with the breeding history of the populations. For instance, unselected populations were found to be more polymorphic than selected breeds such as layers. Thus DNA pools are effective in the preliminary assessment of genetic variation of populations and markers. Mean genetic distance indicates the extent to which a given population shares its genetic diversity with that of the whole tested gene pool and is a useful criterion for conservation of diversity. The distribution of population-specific (private alleles and the amount of genetic variation shared among populations supports the hypothesis that the red jungle fowl is the main progenitor of the domesticated chicken.

  11. (Microbiological studies of small hot-bath-pools and hot-whirl-pools (author's transl))

    Energy Technology Data Exchange (ETDEWEB)

    Exner, M; Havenith, N

    1981-01-01

    Hot small bathing pools and hot whirl-pools have the following characteristics: small watervolume, thick squeeze of swimmers, high water temperature (37-40 degrees C) and small dimension of filters. By this, the quality of bathing-water is influenced detrimentally. To elaborate the hygienic problems, bathing-water samples were taken before, during and after the visiting-hours and were tested for facultative-pathogenic microorganisms. During this investigation E. coli was isolated in 25 degrees, Coliforms and Proteus species in 37.3%, P. aeruginosa in 36%, S. aureus in 26.3%, Enterococci in 42.3 %, Candida albicans in 3.6% and yeast totally in 8.3%.

  12. The atmospheric wet pool: definition and comparison with the oceanic warm pool

    Institute of Scientific and Technical Information of China (English)

    ZHANG Caiyun; CHEN Ge

    2008-01-01

    The oceanic warm pool (OWP) defined by sea surface temperature (SST) is known as the "heat reservoir" in the ocean. The warmest portion in the ocean mirrors the fact that the wettest region with the largest accumulation of water vapor (WV) in the atmosphere, termed atmospheric wet pool (AWP), should be identified because of the well-known Clausius-Clapeyron relationship between SST and WV. In this study, we used 14-year simultaneous observations of WV and SST from January 1988 to December 2001 to define the AWP and investigate its coupling and co-variations with the OWP. The joint examination of the area variations, centroid locations, and zonal migrations of the AWP and OWP lead to a number of interesting findings. The results hopefully can contribute to our understanding of the air-sea interaction in general and characterization of El Nifio/La Nina events in particular.

  13. Nuclear Insurance Pools: World-wide Practice and Prospective

    International Nuclear Information System (INIS)

    Reitsma, S. M. S.

    2000-01-01

    The following paper explains why Nuclear Insurance Pools were established, how they operate and what insurance protection they offer to the operations of nuclear installations. It will be shown that the clear interrelationship of the Pool-insurance operations, both on a national and an international level, has resulted in a transparency of each individual Pool-Member's exposure, which enables him to make the highest possible commitment to nuclear risks. Finally, some views will be given as regards the future prospective for the long proven method of pooling this particularly sensitive class of business. (author)

  14. A Double Evolutionary Pool Memetic Algorithm for Examination Timetabling Problems

    Directory of Open Access Journals (Sweden)

    Yu Lei

    2014-01-01

    Full Text Available A double evolutionary pool memetic algorithm is proposed to solve the examination timetabling problem. To improve the performance of the proposed algorithm, two evolutionary pools, that is, the main evolutionary pool and the secondary evolutionary pool, are employed. The genetic operators have been specially designed to fit the examination timetabling problem. A simplified version of the simulated annealing strategy is designed to speed the convergence of the algorithm. A clonal mechanism is introduced to preserve population diversity. Extensive experiments carried out on 12 benchmark examination timetabling instances show that the proposed algorithm is able to produce promising results for the uncapacitated examination timetabling problem.

  15. Crust formation and its effect on the molten pool coolability

    Energy Technology Data Exchange (ETDEWEB)

    Park, R.J.; Lee, S.J.; Sim, S.K. [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1995-09-01

    Experimental and analytical studies of the crust formation and its effect on the molten pool coolability have been performed to examine the crust formation process as a function of boundary temperatures as well as to investigate heat transfer characteristics between molten pool and overlying water in order to evaluate coolability of the molten pool. The experimental test results have shown that the surface temperature of the bottom plate is a dominant parameter in the crust formation process of the molten pool. It is also found that the crust thickness of the case with direct coolant injection into the molten pool is greater than that of the case with a heat exchanger. Increasing mass flow rate of direct coolant injection to the molten pool does not affect the temperature of molten pool after the crust has been formed in the molten pool because the crust behaves as a thermal barrier. The Nusselt number between the molten pool and the coolant of the case with no crust formation is greater than that of the case with crust formation. The results of FLOW-3D analyses have shown that the temperature distribution contributes to the crust formation process due to Rayleigh-Benard natural convection flow.

  16. Automated management of engineering infrastructure of pools of different purpose

    Directory of Open Access Journals (Sweden)

    Shirokov Lev Alekseevich

    2016-10-01

    Full Text Available Pools play an important role in people’s life. They answer people’s demand in rest and improve their health. At the same time pools are rather important for industrial use, for example in construction industry. In order to solve different construction problems it is essential to investigate the influence of microclimatic parameters on construction materials and structures. For this aim pools are in demand as special test sites for construction materials and structures in different environmental conditions including the case of a direct water impact. The efficient use of pools presupposes the necessity of constant hydroclimatic contro: air humidity and temperature, water temperature, chemical composition of water and air. Classification of pools of different purposes is presented in the article. The author considers the main problems of operation of pools as objects with complicated air-and-water environment. The questions of maintaining optimal microclimatic parameters in a pool are considered. The necessity of use of the control system of a microclimate, its efficiency, profitability and social effect of its implementation is described. A mathematical model of the thermal mode of a pool area is constructed. The process of indoor temperature regulation in the pool is considered.

  17. Steam blowdown experiments with the condensation pool test rig

    International Nuclear Information System (INIS)

    Purhonen, H.; Puustinen, M.; Laine, J.; Raesaenen, A.; Kyrki-Rajamaeki, R.; Vihavainen, J.

    2005-01-01

    During a possible loss-of-coolant accident (Local) a large amount of non-condensable (nitrogen) and condensable (steam) gas is blown from the upper drywell of the containment to the condensation pool through the blowdown pipes at the boiling water reactors (BWRs). The wet well pool serves as the major heat sink for condensation of steam. The blowdown causes both dynamic and structural loads to the condensation pool. There might also be a risk that the gas discharging to the pool could push its way to the emergency core cooling systems (ECCS) and undermine their performance. (author)

  18. Evaporation-preventive device for nuclear reactor pool water

    International Nuclear Information System (INIS)

    Kurusu, Yoshihisa; Akabori, Shiro.

    1986-01-01

    Purpose: To prevent pool water from evaporating by a great amount in a reactor pool such as a spent fuel storing pool. Constitution: Air discharge and in-take ports are disposed just above the surface of the pool water and charge and discharge of airs are forcively carried out to form air curtains above the pool water. Water vapor evaporated from the surface of the pool water does not diffuse above the air curtains due to the air stream of the curtains, but is intaken into the intake port and then condensated into water by a steam condensator and re-supplied to the pool. Since diffusion of water vapor and radioactive materials are suppressed above the air curtains, the working circumstance in the pool chamber can be maintained desirably thereby keeping the radioactivity dose in the atmosphere. Further, incorporation of dusts from above into the pool can also be prevented by the air curtains to provide an effect for the prevention of radioactive contamination. Further, since covers are not used, visual observation can be insured. (Kawakami, Y.)

  19. Evaporation rate measurement in the pool of IEAR-1 reactor

    International Nuclear Information System (INIS)

    Torres, Walmir Maximo; Cegalla, Miriam A.; Baptista Filho, Benedito Dias

    2000-01-01

    The surface water evaporation in pool type reactors affects the ventilation system operation and the ambient conditions and dose rates in the operation room. This paper shows the results of evaporation rate experiment in the pool of IEA-R1 research reactor. The experiment is based on the demineralized water mass variation inside cylindrical metallic recipients during a time interval. Other parameters were measured, such as: barometric pressure, relative humidity, environmental temperature, water temperature inside the recipients and water temperature in the reactor pool. The pool level variation due to water contraction/expansion was calculated. (author)

  20. Livestock Grazing as a Driver of Vernal Pool Ecohydrology

    Science.gov (United States)

    Michaels, J.; McCarten, N. F.

    2017-12-01

    Vernal pools are seasonal wetlands that host rare plant communities of high conservation priority. Plant community composition is largely driven by pool hydroperiod. A previous study found that vernal pools grazed by livestock had longer hydroperiods compared with pools excluded from grazing for 10 years, and suggests that livestock grazing can be used to protect plant diversity. It is important to assess whether observed differences are due to the grazing or due to water balance variables including upland discharge into or out of the pools since no a priori measurements were made of the hydrology prior to grazing. To address this question, in 2016 we compared 15 pools that have been grazed continuously and 15 pools that have been fenced off for over 40 years at a site in Sacramento County. We paired pools based on abiotic characteristics (size, shape, slope, soil type) to minimize natural variation. We sampled vegetation and water depth using Solinst level loggers. We found that plant diversity and average hydroperiod was significantly higher in the grazed pools. We are currently measuring groundwater connectivity and upland inputs in order to compare the relative strength of livestock grazing as a driver of hydroperiod to these other drivers.

  1. Pooling birth cohorts in allergy and asthma

    DEFF Research Database (Denmark)

    Bousquet, Jean; Anto, Josep; Sunyer, Jordi

    2013-01-01

    Long-term birth cohort studies are essential to understanding the life course and childhood predictors of allergy and the complex interplay between genes and the environment (including lifestyle and socioeconomic determinants). Over 100 cohorts focusing on asthma and allergy have been initiated...

  2. A Comprehensive tRNA Deletion Library Unravels the Genetic Architecture of the tRNA Pool

    Science.gov (United States)

    Bloom-Ackermann, Zohar; Navon, Sivan; Gingold, Hila; Towers, Ruth; Pilpel, Yitzhak; Dahan, Orna

    2014-01-01

    Deciphering the architecture of the tRNA pool is a prime challenge in translation research, as tRNAs govern the efficiency and accuracy of the process. Towards this challenge, we created a systematic tRNA deletion library in Saccharomyces cerevisiae, aimed at dissecting the specific contribution of each tRNA gene to the tRNA pool and to the cell's fitness. By harnessing this resource, we observed that the majority of tRNA deletions show no appreciable phenotype in rich medium, yet under more challenging conditions, additional phenotypes were observed. Robustness to tRNA gene deletion was often facilitated through extensive backup compensation within and between tRNA families. Interestingly, we found that within tRNA families, genes carrying identical anti-codons can contribute differently to the cellular fitness, suggesting the importance of the genomic surrounding to tRNA expression. Characterization of the transcriptome response to deletions of tRNA genes exposed two disparate patterns: in single-copy families, deletions elicited a stress response; in deletions of genes from multi-copy families, expression of the translation machinery increased. Our results uncover the complex architecture of the tRNA pool and pave the way towards complete understanding of their role in cell physiology. PMID:24453985

  3. Total quality in spent fuel pool reracking

    International Nuclear Information System (INIS)

    Cranston, J.S.; Bradbury, R.B.; Cacciapouti, R.J.

    1993-01-01

    The nuclear utility environment is one of strict cost control under prescriptive regulations and increasing public scrutiny. This paper presents the results of A Total Quality approach, by a dedicated team, that addresses the need for increased on-site spent fuel storage in this environment. Innovations to spent fuel pool reracking, driven by utilities' specific technical needs and shrinking budgets, have resulted in both product improvements and lower prices. A Total Quality approach to the entire turnkey project is taken, thereby creating synergism and process efficiency in each of the major phases of the project: design and analysis, licensing, fabrication, installation and disposal. Specific technical advances and the proven quality of the team members minimizes risk to the utility and its shareholders and provides a complete, cost effective service. Proper evaluation of spent fuel storage methods and vendors requires a full understanding of currently available customer driven initiatives that reduce cost while improving quality. In all phases of a spent fuel reracking project, from new rack design and analysis through old rack disposal, the integration of diverse experts, at all levels and throughout all phases of a reracking project, better serves utility needs. This Total Quality environment in conjunction with many technical improvements results in a higher quality product at a lower cost

  4. Positive year for Alberta power pool

    International Nuclear Information System (INIS)

    Reid-Carlson, D.

    1997-01-01

    The electricity power pool in Alberta completed its first year under deregulation. Results to date indicate that the competitive market has operated as intended. The effects of electricity pricing on the oil industry following deregulation were described, given the fact that electricity prices represent the second largest cost item to the oil industry after labour. The peculiarities of the mechanism of electricity pricing (based on hourly matching of supply offers to demand bids) were explained, highlighting the opportunities and risks to the oil industry caused by the hourly price variations and the difficulties involved in accurately forecasting on-peak and off-peak prices a full year in advance. In 1996 predicted average price was $14 to $17/MWh. The actual average price was $13.40/MWh. The general conclusion was that Alberta continues to have a surplus of electricity generation and is well positioned to to take advantage of its low generating costs, at least over the longer term. Short term bidding practices, however, may results in slightly higher system marginal prices

  5. Pool critical assembly pressure vessel facility benchmark

    International Nuclear Information System (INIS)

    Remec, I.; Kam, F.B.K.

    1997-07-01

    This pool critical assembly (PCA) pressure vessel wall facility benchmark (PCA benchmark) is described and analyzed in this report. Analysis of the PCA benchmark can be used for partial fulfillment of the requirements for the qualification of the methodology for pressure vessel neutron fluence calculations, as required by the US Nuclear Regulatory Commission regulatory guide DG-1053. Section 1 of this report describes the PCA benchmark and provides all data necessary for the benchmark analysis. The measured quantities, to be compared with the calculated values, are the equivalent fission fluxes. In Section 2 the analysis of the PCA benchmark is described. Calculations with the computer code DORT, based on the discrete-ordinates method, were performed for three ENDF/B-VI-based multigroup libraries: BUGLE-93, SAILOR-95, and BUGLE-96. An excellent agreement of the calculated (C) and measures (M) equivalent fission fluxes was obtained. The arithmetic average C/M for all the dosimeters (total of 31) was 0.93 ± 0.03 and 0.92 ± 0.03 for the SAILOR-95 and BUGLE-96 libraries, respectively. The average C/M ratio, obtained with the BUGLE-93 library, for the 28 measurements was 0.93 ± 0.03 (the neptunium measurements in the water and air regions were overpredicted and excluded from the average). No systematic decrease in the C/M ratios with increasing distance from the core was observed for any of the libraries used

  6. Iodine release from sodium pool combustion

    International Nuclear Information System (INIS)

    Sagawa, N.; Fukushima, Y.; Yokota, N.; Akagane, K.; Mochizuki, K.

    1979-01-01

    Iodine release associated with sodium pool combustion was determined by heating 20 gr sodium containing sodium iodide, which was labelled with 131 I and dissolved in the sodium in concentration of 1∼1,000 ppm, to burn on a nickel crucible in conditioned atmosphere in a closed vessel of 0.4 m 3 . Oxygen concentration was changed in 5∼21% and humidity in 0∼89% by mixing nitrogen gas and air. Combustion products were trapped by a Maypack filter composed of particle filters, copper screens and activated charcoal beds and by a glass beads pack cooled by liquid argon. Iodine collected on these filter elements was determined by radio-gas chromatography. When the sodium sample burned in the atmosphere of air at room temperature, the release fractions observed were 6∼33% for sodium and 1∼20% for iodine added in the sodium. The release iodine was present in aerosol at a ratio of 98%, and the remainder in the gas form. The release fraction of iodine trended to decrease as oxygen concentration and humidity in the atmosphere increased. No organic iodide was detected in the combustion products. (author)

  7. Swimming pools as heat sinks for air conditioners: Model design and experimental validation for natural thermal behavior of the pool

    Energy Technology Data Exchange (ETDEWEB)

    Woolley, Jonathan; Harrington, Curtis; Modera, Mark [University of California Davis, Western Cooling Efficiency Center, 1450 Drew Avenue, Suite 100, Davis, CA 95618 (United States)

    2011-01-15

    Swimming pools as thermal sinks for air conditioners could save approximately 40% on peak cooling power and 30% of overall cooling energy, compared to standard residential air conditioning. Heat dissipation from pools in semi-arid climates with large diurnal temperature shifts is such that pool heating and space cooling may occur concurrently; in which case heat rejected from cooling equipment could directly displace pool heating energy, while also improving space cooling efficiency. The performance of such a system relies on the natural temperature regulation of swimming pools governed by evaporative and convective heat exchange with the air, radiative heat exchange with the sky, and conductive heat exchange with the ground. This paper describes and validates a model that uses meteorological data to accurately predict the hourly temperature of a swimming pool to within 1.1 C maximum error over the period of observation. A thorough review of literature guided our choice of the most appropriate set of equations to describe the natural mass and energy exchange between a swimming pool and the environment. Monitoring of a pool in Davis, CA, was used to confirm the resulting simulations. Comparison of predicted and observed pool temperature for all hours over a 56 day experimental period shows an R-squared relatedness of 0.967. (author)

  8. Macroinvertebrate community assembly in pools created during peatland restoration.

    Science.gov (United States)

    Brown, Lee E; Ramchunder, Sorain J; Beadle, Jeannie M; Holden, Joseph

    2016-11-01

    Many degraded ecosystems are subject to restoration attempts, providing new opportunities to unravel the processes of ecological community assembly. Restoration of previously drained northern peatlands, primarily to promote peat and carbon accumulation, has created hundreds of thousands of new open water pools. We assessed the potential benefits of this wetland restoration for aquatic biodiversity, and how communities reassemble, by comparing pool ecosystems in regions of the UK Pennines on intact (never drained) versus restored (blocked drainage-ditches) peatland. We also evaluated the conceptual idea that comparing reference ecosystems in terms of their compositional similarity to null assemblages (and thus the relative importance of stochastic versus deterministic assembly) can guide evaluations of restoration success better than analyses of community composition or diversity. Community composition data highlighted some differences in the macroinvertebrate composition of restored pools compared to undisturbed peatland pools, which could be used to suggest that alternative end-points to restoration were influenced by stochastic processes. However, widely used diversity metrics indicated no differences between undisturbed and restored pools. Novel evaluations of restoration using null models confirmed the similarity of deterministic assembly processes from the national species pool across all pools. Stochastic elements were important drivers of between-pool differences at the regional-scale but the scale of these effects was also similar across most of the pools studied. The amalgamation of assembly theory into ecosystem restoration monitoring allows us to conclude with more certainty that restoration has been successful from an ecological perspective in these systems. Evaluation of these UK findings compared to those from peatlands across Europe and North America further suggests that restoring peatland pools delivers significant benefits for aquatic fauna by

  9. Microbial contributions to coupled arsenic and sulfur cycling in the acid-sulfide hot spring Champagne Pool, New Zealand

    Directory of Open Access Journals (Sweden)

    Katrin eHug

    2014-11-01

    Full Text Available Acid-sulfide hot springs are analogs of early Earth geothermal systems where microbial metal(loid resistance likely first evolved. Arsenic is a metalloid enriched in the acid-sulfide hot spring Champagne Pool (Waiotapu, New Zealand. Arsenic speciation in Champagne Pool follows reaction paths not yet fully understood with respect to biotic contributions and coupling to biogeochemical sulfur cycling. Here we present quantitative arsenic speciation from Champagne Pool, finding arsenite dominant in the pool, rim and outflow channel (55-75% total arsenic, and dithio- and trithioarsenates ubiquitously present as 18-25% total arsenic. In the outflow channel, dimethylmonothioarsenate comprised ≤9% total arsenic, while on the outflow terrace thioarsenates were present at 55% total arsenic. We also quantified sulfide, thiosulfate, sulfate and elemental sulfur, finding sulfide and sulfate as major species in the pool and outflow terrace, respectively. Elemental sulfur reached a maximum at the terrace. Phylogenetic analysis of 16S rRNA genes from metagenomic sequencing revealed the dominance of Sulfurihydrogenibium at all sites and an increased archaeal population at the rim and outflow channel. Several phylotypes were found closely related to known sulfur- and sulfide-oxidizers, as well as sulfur- and sulfate-reducers. Bioinformatic analysis revealed genes underpinning sulfur redox transformations, consistent with sulfur speciation data, and illustrating a microbial role in sulfur-dependent transformation of arsenite to thioarsenate. Metagenomic analysis also revealed genes encoding for arsenate reductase at all sites, reflecting the ubiquity of thioarsenate and a need for microbial arsenate resistance despite anoxic conditions. Absence of the arsenite oxidase gene, aio, at all sites suggests prioritization of arsenite detoxification over coupling to energy conservation. Finally, detection of methyl arsenic in the outflow channel, in conjunction with

  10. 76 FR 72923 - Pool Corporation; Analysis To Aid Public Comment

    Science.gov (United States)

    2011-11-28

    ... manufacturers, warehouse them, and then resell the products to pool retail stores, pool service companies and.... ADDRESSES: Interested parties may file a comment online or on paper, by following the instructions in the... 0115'' on your comment, and file your comment online at https://ftcpublic.commentworks.com/ftc...

  11. Solar collectors for swimming pools still going strong

    Energy Technology Data Exchange (ETDEWEB)

    1975-01-01

    According to the opinion of the experts, solar energy heating may be technically 'mature' but the profitability is by no means that far. However, solar systems are a good alternative for heating the water in swimming pools. Four solar collector systems developed by different firms to heat swimming pools, including prices, are presented.

  12. Microscopic bubble behaviour in suppression pool during wetwell venting

    Science.gov (United States)

    Zablackaite, G.; Nagasaka, H.; Kikura, H.

    2017-10-01

    During a severe accident PCV failure should be avoided and fission products inside PCV should be confined as much as possible. In order to minimize FPs release, Wetwell venting is conducted by releasing steam-non-condensable gas mixture carrying FPs from the Drywell to Suppression Pool. Steam is condensed by subcooled water in the pool, and most of FPs are retained into water. The removal of FP in the water pool is referred to as “Pool Scrubbing effect”. Hydrodynamic parameters of bubbles have impact on pool scrubbing effect. However, there is only few data available to evaluate quantitatively the bubble behaviour under depressurization and/or thermal stratification conditions. Series of experiments were conducted to evaluate the influence of temperature distribution, non-condensable gas content and pressure in the Wetwell on bubble behaviour. Bubbles were visualized using High Speed Camera and adopting shadowgraphy technique. Applying Particle Tracking Velocimetry, bubble velocity and size distribution were obtained from recorded images. Experimental results show that with increasing suppression pool temperature, bubbles reaching the pool surface decreased in size and traveling velocity became slower. In pressurized wetwell, bubble behaviour was similar to that in the heated up suppression pool case, although bubble parameters were similar to the low temperature case. Higher air content induced water surface movement and bubbles were smaller due to break up.

  13. 48 CFR 28.304 - Risk-pooling arrangements.

    Science.gov (United States)

    2010-10-01

    ... CONTRACTING REQUIREMENTS BONDS AND INSURANCE Insurance 28.304 Risk-pooling arrangements. Agencies may establish risk-pooling arrangements. These arrangements are designed to use the services of the insurance industry for safety engineering and the handling of claims at minimum cost to the Government. The agency...

  14. Biomass and carbon pools of disturbed riparian forests

    Science.gov (United States)

    Laura A. B. Giese; W. M. Aust; Randall K. Kolka; Carl C. Trettin

    2003-01-01

    Quantification of carbon pools as affected by forest age/development can facilitate riparian restoration and increase awareness of the potential for forests to sequester global carbon. Riparian forest biomass and carbon pools were quantified for four riparian forests representing different seral stages in the South Carolina Upper Coastal Plain. Three of the riparian...

  15. 17 CFR 275.206(4)-8 - Pooled investment vehicles.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Pooled investment vehicles... COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT ADVISERS ACT OF 1940 § 275.206(4)-8 Pooled investment vehicles. (a) Prohibition. It shall constitute a fraudulent, deceptive, or manipulative act...

  16. Mathematical-programming approaches to test item pool design

    NARCIS (Netherlands)

    Veldkamp, Bernard P.; van der Linden, Willem J.; Ariel, A.

    2002-01-01

    This paper presents an approach to item pool design that has the potential to improve on the quality of current item pools in educational and psychological testing andhence to increase both measurement precision and validity. The approach consists of the application of mathematical programming

  17. Anticipated corrosion in the Vermont Yankee spent fuel pool

    International Nuclear Information System (INIS)

    Weeks, J.R.

    1977-06-01

    The report provides additional information relating to a proposed modification to the spent fuel pool at the Vermont Yankee Nuclear Power Station (VYNPS) and addresses corrosion of spent fuel pool storage materials and zircaloy, and provides an analysis of the effectiveness of the Boral sealing

  18. Numerical analysis of weld pool oscillation in laser welding

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Jung Ho [Chungbuk National University, Cheongju (Korea, Republic of); Farson, Dave F [The Ohio State University, Columbus (United States); Hollis, Kendall; Milewski, John O. [Los Alamos National Laboratory, Los Alamos (United States)

    2015-04-15

    Volume of fluid (VOF) numerical simulation was used to investigate melt flow and volumetric oscillation of conduction-mode pulsed laser weld pools. The result is compared to high speed video stream of titanium laser spot welding experiment. The total simulation time is 10ms with the first 5 ms being heating and melting under constant laser irradiation and the remaining 5 ms corresponding to resolidification of the weld pool. During the melting process, the liquid pool did not exhibit periodic oscillation but was continually depressed by the evaporation recoil pressure. After the laser pulse, the weld pool was excited into volumetric oscillation by the release of pressure on its surface and oscillation of the weld pool surface was analyzed. The simulation model suggested adjusting thermal diffusivity to match cooling rate and puddle diameter during solidification which is distinguishable from previous weld pool simulation. The frequency continuously increased from several thousand cycles per second to tens of thousands of cycles per second as the weld pool solidified and its diameter decreased. The result is the first trial of investigation of small weld pool oscillation in laser welding although there have been several reports about arc welding.

  19. 13 CFR 120.1709 - Transfers of Pool Certificates.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Transfers of Pool Certificates. 120.1709 Section 120.1709 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools § 120.1709...

  20. 13 CFR 120.1714 - Seller's Pool Loan servicing.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Seller's Pool Loan servicing. 120.1714 Section 120.1714 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools § 120.1714 Seller...

  1. 13 CFR 120.1715 - Seller's Pool Loan liquidation.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Seller's Pool Loan liquidation. 120.1715 Section 120.1715 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools § 120.1715 Seller...

  2. 13 CFR 120.1713 - Seller's Pool Loan origination.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Seller's Pool Loan origination. 120.1713 Section 120.1713 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools § 120.1713 Seller...

  3. 13 CFR 120.1717 - Seller's Pool Loan deferments.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Seller's Pool Loan deferments. 120.1717 Section 120.1717 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools § 120.1717 Seller...

  4. AUS burnup module CHAR and the associated data pool

    International Nuclear Information System (INIS)

    Robinson, G.S.

    1975-12-01

    The CHAR module of the AUS reactor neutronics scheme solves the multiregion nuclide depletion equations using an analytic method. The module obtains cross section, flux and geometry data from AUS data pools, and uses the STATUS data pool which has been designed for the storage of nuclide compositions, spatial smearing factors and other miscellaneous information. (author)

  5. Phytomass carbon pool of trees and forests in India

    NARCIS (Netherlands)

    Kaul, M.; Mohren, G.M.J.; Dadhwal, V.K.

    2011-01-01

    The study reports estimates of above ground phytomass carbon pools in Indian forests for 1992 and 2002 using two different methodologies. The first estimate was derived from remote sensing based forest area and crown density estimates, and growing stock data for 1992 and 2002 and the estimated pool

  6. Odonata (Insecta at a wadi Pool near Nizwa, northern Oman

    Directory of Open Access Journals (Sweden)

    Elaine M. Cowan

    2015-07-01

    Full Text Available Fourteen damselfly and dragonfly species were recorded in 68 visits to a wadi pool in northern Oman, March 2012 to June 2014.  All identifications were based on photographs.  Apparently the pool has a core community of eight resident species.  Paragomphus sinaiticus, globally Near Threatened, was regularly  recorded. 

  7. Electricity prices and generator behaviour in gross pool electricity markets

    International Nuclear Information System (INIS)

    O'Mahoney, Amy; Denny, Eleanor

    2013-01-01

    Electricity market liberalisation has become common practice internationally. The justification for this process has been to enhance competition in a market traditionally characterised by statutory monopolies in an attempt to reduce costs to end-users. This paper endeavours to see whether a pool market achieves this goal of increasing competition and reducing electricity prices. Here the electricity market is set up as a sealed bid second price auction. Theory predicts that such markets should result with firms bidding their marginal cost, thereby resulting in an efficient outcome and lower costs to consumers. The Irish electricity system with a gross pool market experiences among the highest electricity prices in Europe. Thus, we analyse the Irish pool system econometrically in order to test if the high electricity prices seen there are due to participants bidding outside of market rules or out of line with theory. Overall we do not find any evidence that the interaction between generator and the pool in the Irish electricity market is not efficient. Thus, the pool element of the market structure does not explain the high electricity prices experienced in Ireland. - Highlights: • We consider whether a gross pool achieves competitive behaviour. • We analyse the Irish pool system econometrically. • Results indicate the Irish pool system appears to work efficiently. • Generators appear to be bidding appropriately

  8. Branchipodopsis species — specialists of ephemeral rock pools ...

    African Journals Online (AJOL)

    Such rock pools were studied in the Drakensberg region, in the eastern Free State and in south-eastern Botswana. Common features ... While short-range dispersal of floating eggs by overflows is common, long-range dispersal (e.g. by wind) seems to be rare and to be restricted to shallow pools with little vegetation. Limited ...

  9. Characteristics of fission product release from a molten pool

    International Nuclear Information System (INIS)

    Yun, J.I.; Suh, K.Y.; Kang, C.S.

    2001-01-01

    The volatile fission products are released from the debris pool, while the less volatile fission products tend to remain as condensed phases because of their low vapor pressure. The release of noble gases and the volatile fission products is dominated by bubble dynamics. The release of the less volatile fission products from the pool can be analyzed based on mass transport through a liquid with the convection flow. The physico-numerical models were orchestrated from existing submodels in various disciplines of engineering to estimate the released fraction of fission products from a molten pool. It was assumed that the pool has partially filled hemispherical geometry. For the high pool pressure, the diameter of the bubbles at detachment was calculated utilizing the Cole and Shulman correlation with the effect of system pressure. Sensitivity analyses were performed and results of the numerical calculations were compared with analysis results for the TMI-2 accident. (author)

  10. Tax credits and purchasing pools: will this marriage work?

    Science.gov (United States)

    Trude, S; Ginsburg, P B

    2001-04-01

    Bipartisan interest is growing in Congress for using federal tax credits to help low-income families buy health insurance. Regardless of the approach taken, tax credit policies must address risk selection issues to ensure coverage for the chronically ill. Proposals that link tax credits to purchasing pools would avoid risk selection by grouping risks similar to the way large employers do. Voluntary purchasing pools have had only limited success, however. This Issue Brief discusses linking tax credits to purchasing pools. It uses information from the Center for Studying Health System Change's (HSC) site visits to 12 communities as well as other research to assess the role of purchasing pools nationwide and the key issues and implications of linking tax credits and pools.

  11. Controlling pool depth during VAR of Alloy 718

    Science.gov (United States)

    Lopez, F.; Beaman, J.; Williamson, R.; Evans, D.

    2016-07-01

    A longtime goal of superalloy producers has been to control the geometry of the liquid pool in solidifying ingots. Accurate pool depth control at appropriate values is expected to result in ingots free of segregation defects. This article describes an industrial VAR experiment in which a 430mm (17 in) diameter Alloy 718 electrode was melted into a 510mm (20 in) ingot. In the experiment, the depth of the liquid pool at the mid-radius was controlled to three different set-points: 137 mm (nominal), 193 mm (deep) and 118 mm (shallow). At each level, the pool depth was marked by a power cutback of several minutes. The ingot was sectioned and longitudinal slices were cut out. Analysis of the photographed ingot revealed that accurate control was obtained for both the nominal and deep pool cases, while the third one was not conclusive.

  12. Parametric tomography of the cardiac blood pool

    International Nuclear Information System (INIS)

    Meyer, M.; Schwartz, K.D.

    1990-01-01

    In nuclear cardiology image processing is performed usually in 3 of 4 dimensions. ECG-gated SPECT (GSPECT) would make it possible to obtain all 4 dimensions of space and time during one examination, but its duration as well as radiation dose is limited resulting in a low signal-to-noise ratio. Sensitive feature extractions from the amount of data are necessary, e.g. Fourier filtering or extracting isovolumetric intervals. The relatively large amount of calculations and storage requirements often handicaps tomographic ventriculography because a high number of sections have to be processed and the temporal resolution is limited. A new list-mode oriented tomographic algorithm demands less storage and fewer calculations: The Fourier coefficient extraction and the filtered back projection, both of which are linear operations, could be interchanged in the case of thoracic SPECT. The feature extraction algorithm process internal list-mode heart cycles for discrimination of invalid cycles, for end-diastolic and end-systolic synthesis as well as for Fourier analysis of the first harmonic in 10 ms steps. Reconstruction operations are applied also to modified distribution matrices of Fourier coefficients. By only processing 4 spatial matrix sequences (end-diastolic and end-systolic images, amplitude and phase values) parametric tomography becomes practicable and could be also performed by a minicomputer with 64 KByte memory in addition to the possibilities of the planar left ventricular gated imaging. If there are 3 or more processors available a complete feature extraction on-the-fly will be possible. The numerical algorithms were tested with respect to stable reconstructions by phantoms. First results of a patient examination are used to explore effective display techniques, and preliminary modes are demonstrated. It is the purpose of this study to obtain additional information about the gated planar cardiac blood pool imaging in the field of SPECT. (author)

  13. poolHiTS: A Shifted Transversal Design based pooling strategy for high-throughput drug screening

    Directory of Open Access Journals (Sweden)

    Woolf Peter J

    2008-05-01

    Full Text Available Abstract Background A key goal of drug discovery is to increase the throughput of small molecule screens without sacrificing screening accuracy. High-throughput screening (HTS in drug discovery involves testing a large number of compounds in a biological assay to identify active compounds. Normally, molecules from a large compound library are tested individually to identify the activity of each molecule. Usually a small number of compounds are found to be active, however the presence of false positive and negative testing errors suggests that this one-drug one-assay screening strategy can be significantly improved. Pooling designs are testing schemes that test mixtures of compounds in each assay, thereby generating a screen of the whole compound library in fewer tests. By repeatedly testing compounds in different combinations, pooling designs also allow for error-correction. These pooled designs, for specific experiment parameters, can be simply and efficiently created using the Shifted Transversal Design (STD pooling algorithm. However, drug screening contains a number of key constraints that require specific modifications if this pooling approach is to be useful for practical screen designs. Results In this paper, we introduce a pooling strategy called poolHiTS (Pooled High-Throughput Screening which is based on the STD algorithm. In poolHiTS, we implement a limit on the number of compounds that can be mixed in a single assay. In addition, we show that the STD-based pooling strategy is limited in the error-correction that it can achieve. Due to the mixing constraint, we show that it is more efficient to split a large library into smaller blocks of compounds, which are then tested using an optimized strategy repeated for each block. We package the optimal block selection algorithm into poolHiTS. The MATLAB codes for the poolHiTS algorithm and the corresponding decoding strategy are also provided. Conclusion We have produced a practical version

  14. Evaluation of the prognostic role of centromere 17 gain and HER2/topoisomerase II alpha gene status and protein expression in patients with breast cancer treated with anthracycline-containing adjuvant chemotherapy: pooled analysis of two Hellenic Cooperative Oncology Group (HeCOG) phase III trials

    International Nuclear Information System (INIS)

    Fountzilas, George; Tsolaki, Eleftheria; Televantou, Despina; Timotheadou, Eleni; Koutras, Angelos; Klouvas, George; Samantas, Epaminontas; Pisanidis, Nikolaos; Karanikiotis, Charisios; Sfakianaki, Ioanna; Pavlidis, Nicholas; Dafni, Urania; Gogas, Helen; Linardou, Helena; Kalogeras, Konstantine T; Pectasides, Dimitrios; Dimopoulos, Meletios A; Bobos, Mattheos; Kotoula, Vassiliki; Batistatou, Anna; Xanthakis, Ioannis; Papadimitriou, Christos; Kostopoulos, Ioannis; Koletsa, Triantafillia

    2013-01-01

    The HER2 gene has been established as a valid biological marker for the treatment of breast cancer patients with trastuzumab and probably other agents, such as paclitaxel and anthracyclines. The TOP2A gene has been associated with response to anthracyclines. Limited information exists on the relationship of HER2/TOP2A gene status in the presence of centromere 17 (CEP17) gain with outcome of patients treated with anthracycline-containing adjuvant chemotherapy. Formalin-fixed paraffin-embedded tumor tissue samples from 1031 patients with high-risk operable breast cancer, enrolled in two consecutive phase III trials, were assessed in a central laboratory by fluorescence in situ hybridization for HER2/TOP2A gene amplification and CEP17 gain (CEP17 probe). Amplification of HER2 and TOP2A were defined as a gene/CEP17 ratio of >2.2 and ≥2.0, respectively, or gene copy number higher than 6. Additionally, HER2, TopoIIa, ER/PgR and Ki67 protein expression was assessed by immunohistochemistry (IHC) and patients were classified according to their IHC phenotype. Treatment consisted of epirubicin-based adjuvant chemotherapy followed by hormonal therapy and radiation, as indicated. HER2 amplification was found in 23.7% of the patients and TOP2A amplification in 10.1%. In total, 41.8% of HER2-amplified tumors demonstrated TOP2A co-amplification. The median (range) of HER2, TOP2A and CEP17 gain was 2.55 (0.70-45.15), 2.20 (0.70-26.15) and 2.00 (0.70-26.55), respectively. Forty percent of the tumors had CEP17 gain (51% of those with HER2 amplification). Adjusting for treatment groups in the Cox model, HER2 amplification, TOP2A amplification, CEP17 gain and HER2/TOP2A co-amplification were not associated with time to relapse or time to death. HER2 amplification, TOP2A amplification, CEP17 gain and HER2/TOP2A co-amplification were not associated with outcome in high-risk breast cancer patients treated with anthracycline-based adjuvant chemotherapy. Australian New Zealand Clinical

  15. Sample pooling for real-time PCR detection and virulence determination of the footrot pathogen Dichelobacter nodosus.

    Science.gov (United States)

    Frosth, Sara; König, Ulrika; Nyman, Ann-Kristin; Aspán, Anna

    2017-09-01

    Dichelobacter nodosus is the principal cause of ovine footrot and strain virulence is an important factor in disease severity. Therefore, detection and virulence determination of D. nodosus is important for proper diagnosis of the disease. Today this is possible by real-time PCR analysis. Analysis of large numbers of samples is costly and laborious; therefore, pooling of individual samples is common in surveillance programs. However, pooling can reduce the sensitivity of the method. The aim of this study was to develop a pooling method for real-time PCR analysis that would allow sensitive detection and simultaneous virulence determination of D. nodosus. A total of 225 sheep from 17 flocks were sampled using ESwabs within the Swedish Footrot Control Program in 2014. Samples were first analysed individually and then in pools of five by real-time PCR assays targeting the 16S rRNA and aprV2/B2 genes of D. nodosus. Each pool consisted of four negative and one positive D. nodosus samples with varying amounts of the bacterium. In the individual analysis, 61 (27.1%) samples were positive in the 16S rRNA and the aprV2/B2 PCR assays and 164 (72.9%) samples were negative. All samples positive in the aprV2/B2 PCR-assay were of aprB2 variant. The pooled analysis showed that all 41 pools were also positive for D. nodosus 16S rRNA and the aprB2 variant. The diagnostic sensitivity for pooled and individual samples was therefore similar. Our method includes concentration of the bacteria before DNA-extraction. This may account for the maintenance of diagnostic sensitivity. Diagnostic sensitivity in the real-time PCR assays of the pooled samples were comparable to the sensitivity obtained for individually analysed samples. Even sub-clinical infections were able to be detected in the pooled PCR samples which is important for control of the disease. This method may therefore be implemented in footrot control programs where it can replace analysis of individual samples.

  16. A Mechanistic Model of Waterfall Plunge Pool Erosion into Bedrock

    Science.gov (United States)

    Scheingross, Joel S.; Lamb, Michael P.

    2017-11-01

    Landscapes often respond to changes in climate and tectonics through the formation and upstream propagation of knickzones composed of waterfalls. Little work has been done on the mechanics of waterfall erosion, and instead most landscape-scale models neglect waterfalls or use rules for river erosion, such as stream power, that may not be applicable to waterfalls. Here we develop a physically based model to predict waterfall plunge pool erosion into rock by abrasion from particle impacts and test the model against flume experiments. Both the model and experiments show that evolving plunge pools have initially high vertical erosion rates due to energetic particle impacts, and erosion slows and eventually ceases as pools deepen and deposition protects the pool floor from further erosion. Lateral erosion can continue after deposition on the pool floor, but it occurs at slow rates that become negligible as pools widen. Our work points to the importance of vertical drilling of successive plunge pools to drive upstream knickzone propagation in homogenous rock, rather than the classic mechanism of headwall undercutting. For a series of vertically drilling waterfalls, we find that upstream knickzone propagation is faster under higher combined water and sediment fluxes and for knickzones composed of many waterfalls that are closely spaced. Our model differs significantly from stream-power-based erosion rules in that steeper knickzones can retreat faster or more slowly depending on the number and spacing of waterfalls within a knickzone, which has implications for interpreting climatic and tectonic history through analysis of river longitudinal profiles.

  17. Addressing data privacy in matched studies via virtual pooling.

    Science.gov (United States)

    Saha-Chaudhuri, P; Weinberg, C R

    2017-09-07

    Data confidentiality and shared use of research data are two desirable but sometimes conflicting goals in research with multi-center studies and distributed data. While ideal for straightforward analysis, confidentiality restrictions forbid creation of a single dataset that includes covariate information of all participants. Current approaches such as aggregate data sharing, distributed regression, meta-analysis and score-based methods can have important limitations. We propose a novel application of an existing epidemiologic tool, specimen pooling, to enable confidentiality-preserving analysis of data arising from a matched case-control, multi-center design. Instead of pooling specimens prior to assay, we apply the methodology to virtually pool (aggregate) covariates within nodes. Such virtual pooling retains most of the information used in an analysis with individual data and since individual participant data is not shared externally, within-node virtual pooling preserves data confidentiality. We show that aggregated covariate levels can be used in a conditional logistic regression model to estimate individual-level odds ratios of interest. The parameter estimates from the standard conditional logistic regression are compared to the estimates based on a conditional logistic regression model with aggregated data. The parameter estimates are shown to be similar to those without pooling and to have comparable standard errors and confidence interval coverage. Virtual data pooling can be used to maintain confidentiality of data from multi-center study and can be particularly useful in research with large-scale distributed data.

  18. Preliminary Calculation on a Spent Fuel Pool Accident using GOTHIC

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jaehwan; Choi, Yu Jung; Hong, Tae Hyub; Kim, Hyeong-Taek [KHNP-CRI, Daejeon (Korea, Republic of)

    2015-10-15

    The probability of an accident happening at the spent fuel pool was believed to be quite low until the 2011 Fukushima accident occurred. Notably, large amount of spent fuel are normally stored in the spent fuel pool for a long time compared to the amount of fuel in the reactor core and the total heat released from the spent fuel is high enough to boil the water of the spent fuel pool when the cooling system does not operate. In addition, the enrichment and the burnup of the fuel have both increased in the past decade and heat generation from the spent fuel thereby has also increased. The failure of the cooling system at the spent fuel pool (hereafter, a loss-of-cooling accident) is one of the principal hypothetical causes of an accident that could occur at the spent fuel pool. In this paper, the preliminary calculation of a loss-of-cooling accident was performed. In this paper, the preliminary calculation of a loss-of cooling accident was performed with GOTHIC. The calculation results show boiling away of water in the spent fuel pool due to the loss-of-cooling accident and similar thermal performance of the spent fuel pool with previous research results.

  19. 3D finite element simulation of TIG weld pool

    Science.gov (United States)

    Kong, X.; Asserin, O.; Gounand, S.; Gilles, P.; Bergheau, J. M.; Medale, M.

    2012-07-01

    The aim of this paper is to propose a three-dimensional weld pool model for the moving gas tungsten arc welding (GTAW) process, in order to understand the main factors that limit the weld quality and improve the productivity, especially with respect to the welding speed. Simulation is a very powerful tool to help in understanding the physical phenomena in the weld process. A 3D finite element model of heat and fluid flow in weld pool considering free surface of the pool and traveling speed has been developed for the GTAW process. Cast3M software is used to compute all the governing equations. The free surface of the weld pool is calculated by minimizing the total surface energy. The combined effects of surface tension gradient, buoyancy force, arc pressure, arc drag force to drive the fluid flow is included in our model. The deformation of the weld pool surface and the welding speed affect fluid flow, heat flow and thus temperature gradients and molten pool dimensions. Welding trials study is presented to compare our numerical results with macrograph of the molten pool.

  20. Study on water evaporation rate from indoor swimming pools

    Directory of Open Access Journals (Sweden)

    Rzeźnik Ilona

    2017-01-01

    Full Text Available The air relative humidity in closed spaces of indoor swimming pools influences significantly on users thermal comfort and the stability of the building structure, so its preservation on suitable level is very important. For this purpose, buildings are equipped with HVAC systems which provide adequate level of humidity. The selection of devices and their technical parameters is made using the mathematical models of water evaporation rate in the unoccupied and occupied indoor swimming pool. In the literature, there are many papers describing this phenomena but the results differ from each other. The aim of the study was the experimental verification of published models of evaporation rate in the pool. The tests carried out on a laboratory scale, using model of indoor swimming pool, measuring 99cm/68cm/22cm. The model was equipped with water spray installation with six nozzles to simulate conditions during the use of the swimming pool. The measurements were made for conditions of sports pools (water temperature 24°C and recreational swimming pool (water temperature 34°C. According to the recommendations the air temperature was about 2°C higher than water temperature, and the relative humidity ranged from 40% to 55%. Models Shah and Biasin & Krumm were characterized by the best fit to the results of measurements on a laboratory scale.

  1. Security cost allocation under combined bilateral-pool market dispatch

    International Nuclear Information System (INIS)

    Abdullah, M.P.; Hassan, M.Y.; Hussin, F.

    2008-01-01

    Most electricity markets around the world are a combination of bilateral and pool markets, such as NordPool and NYPOOL. In these models, market participants bid into the pool and also make bilateral contracts with each other. This paper addressed the issue of congestion management and security cost allocation in a power pool market model. The basic idea of security cost allocation is to divide the incurred security cost due to congestion relief into pool and bilateral market based on their flow contribution to the congested line. A newly proposed security cost allocation strategy of the combined bilateral-pool market was also presented along with case studies using IEEE-14 bus system that tested the proposed method. Using the proposed method, it was shown that security costs are allocated to market participants at different prices which reflect the load contribution to the security problem. This solves the problem of the uniform security cost allocation in a pure pool market system having uniform pricing, and provides a proper security signal to market participants. 11 refs., 3 tabs., 4 figs

  2. Loss of spent fuel pool cooling PRA: Model and results

    International Nuclear Information System (INIS)

    Siu, N.; Khericha, S.; Conroy, S.; Beck, S.; Blackman, H.

    1996-09-01

    This letter report documents models for quantifying the likelihood of loss of spent fuel pool cooling; models for identifying post-boiling scenarios that lead to core damage; qualitative and quantitative results generated for a selected plant that account for plant design and operational practices; a comparison of these results and those generated from earlier studies; and a review of available data on spent fuel pool accidents. The results of this study show that for a representative two-unit boiling water reactor, the annual probability of spent fuel pool boiling is 5 x 10 -5 and the annual probability of flooding associated with loss of spent fuel pool cooling scenarios is 1 x 10 -3 . Qualitative arguments are provided to show that the likelihood of core damage due to spent fuel pool boiling accidents is low for most US commercial nuclear power plants. It is also shown that, depending on the design characteristics of a given plant, the likelihood of either: (a) core damage due to spent fuel pool-associated flooding, or (b) spent fuel damage due to pool dryout, may not be negligible

  3. Study on velocity distribution in a pool by submersible mixers

    International Nuclear Information System (INIS)

    Tian, F; Shi, W D; Lu, X N; Chen, B; Jiang, H

    2012-01-01

    To study the distribution of submersible mixers and agitating effect in the sewage treatment pool, Pro/E software was utilized to build the three-dimensional model. Then, the large-scale computational fluid dynamics software FLUENT6.3 was used. ICEM software was used to build unstructured grid of sewage treatment pool. After that, the sewage treatment pool was numerically simulated by dynamic coordinate system technology and RNG k-ε turbulent model and PIOS algorithm. The macro fluid field and each section velocity flow field distribution were analyzed to observe the efficiency of each submersible mixer. The average velocity and mixing area in the sewage pool were studied simultaneously. Results show that: the preferred project B, two submersible mixers speed is 980 r/min, and setting angles are all 30°. Fluid mixing area in the pool has reached more than 95%. Under the action of two mixers, the fluid in the sewage pool form a continuous circulating water flow. The fluid is mixed adequately and average velocity of fluid in the pool is at around 0.241m/s, which agreed with the work requirements. Consequently it can provide a reference basis for practical engineering application of submersible mixers by using this method.

  4. Mathematical modeling of the energy consumption of heated swimming pools

    Energy Technology Data Exchange (ETDEWEB)

    Le Bel, C.; Millette, J. [LTE Shawinigan, Shawinigan, PQ (Canada)

    2007-07-01

    A mathematical model was developed to estimate the water temperature of a residential swimming pool. The model can compare 2 different situations and, if local climatic conditions are known, it can accurately predict energy costs of the pool relative to the total energy consumption of the house. When used with the appropriate energy transfer coefficient and weather file, the model can estimate the water temperature of a residential swimming pool having specific characteristics, such as in-ground, above-ground, heated or non-heated. The model is suitable for determining residential loads. It can be applied to different pool types and sizes, for different water heating scenarios and different climatic regions. Data obtained from the monitoring of water temperature and electricity use of 57 residential swimming pools was used to validate the model. In addition, 5 above-ground pools were installed on the property of LTE Shawinigan to allow for a more detailed study of the parameters involved in the thermal balance of a pool. The mathematical model, based on a global heat transfer coefficient, can determine the effect of a solar blanket and the effect of water volume. 14 refs., 5 tabs., 11 figs.

  5. ENERGY SAVING AT OPERATION OF OUTDOOR SWIMMING POOLS

    Directory of Open Access Journals (Sweden)

    V. F. Ivin

    2013-09-01

    Full Text Available Purpose. Energy saving is a major problem in modern power engineering and various energy-consuming devices. They include outdoor swimming pools. In order to maintain them in working condition, especially in winter period, it takes significant amount of thermal energy. Task of heat loss substantial decrease in open swimming pools is considered in the article (on DNURT example. Methodology. The method of determining the mass and heat loss on the basis of criteria equations of heat and mass transfer theory is used. Findings. Calculations of the actual DNURT pool heat loss for different seasons, as for natural convection both for air forced motion above the free water surface are performed. It is shown that for the adiabatic evaporation conditions of water from the pool in winter during blow-off with wind the heat loss can be up to 2 kW/m2 on surface. To reduce these losses it is offered to cover water surface in a pool with a special material with low thermal conductivity on the basis of porous polyethylene during the time when the pool is not used for other purposes. It is shown that the implementation of these standards will reduce the actual heat loss, at least 5-6 times. Originality. The solution of important environmental and energy problem thanks to reducing heat losses by the pool in different times of a year and correspondingly lower emissions of power generating enterprises. Practical value. It is shown that the coating surface of the pool with poorly heat-conducting and easy to install coating will let, at a minimum, to reduce the actual heat loss on 5-6 times and reduce the emissions of power plants generating energy for pool heating.

  6. Pooled-DNA sequencing identifies genomic regions of selection in Nigerian isolates of Plasmodium falciparum.

    Science.gov (United States)

    Oyebola, Kolapo M; Idowu, Emmanuel T; Olukosi, Yetunde A; Awolola, Taiwo S; Amambua-Ngwa, Alfred

    2017-06-29

    The burden of falciparum malaria is especially high in sub-Saharan Africa. Differences in pressure from host immunity and antimalarial drugs lead to adaptive changes responsible for high level of genetic variations within and between the parasite populations. Population-specific genetic studies to survey for genes under positive or balancing selection resulting from drug pressure or host immunity will allow for refinement of interventions. We performed a pooled sequencing (pool-seq) of the genomes of 100 Plasmodium falciparum isolates from Nigeria. We explored allele-frequency based neutrality test (Tajima's D) and integrated haplotype score (iHS) to identify genes under selection. Fourteen shared iHS regions that had at least 2 SNPs with a score > 2.5 were identified. These regions code for genes that were likely to have been under strong directional selection. Two of these genes were the chloroquine resistance transporter (CRT) on chromosome 7 and the multidrug resistance 1 (MDR1) on chromosome 5. There was a weak signature of selection in the dihydrofolate reductase (DHFR) gene on chromosome 4 and MDR5 genes on chromosome 13, with only 2 and 3 SNPs respectively identified within the iHS window. We observed strong selection pressure attributable to continued chloroquine and sulfadoxine-pyrimethamine use despite their official proscription for the treatment of uncomplicated malaria. There was also a major selective sweep on chromosome 6 which had 32 SNPs within the shared iHS region. Tajima's D of circumsporozoite protein (CSP), erythrocyte-binding antigen (EBA-175), merozoite surface proteins - MSP3 and MSP7, merozoite surface protein duffy binding-like (MSPDBL2) and serine repeat antigen (SERA-5) were 1.38, 1.29, 0.73, 0.84 and 0.21, respectively. We have demonstrated the use of pool-seq to understand genomic patterns of selection and variability in P. falciparum from Nigeria, which bears the highest burden of infections. This investigation identified known

  7. Flow dynamics of volume-heated boiling pools

    International Nuclear Information System (INIS)

    Ginsberg, T.; Jones, O.C.; Chen, J.C.

    1979-01-01

    Safety analyses of fast breeder reactors require understanding of the two-phase fluid dynamic and heat transfer characteristics of volume-heated boiling pool systems. Design of direct contact three-phase boilers, of practical interest in the chemical industries also requires understanding of the fundamental two-phase flow and heat transfer behavior of volume boiling systems. Several experiments have been recently reported relevant to the boundary heat-loss mechanisms of boiling pool systems. Considerably less is known about the two-phase fluid dynamic behavior of such systems. This paper describes an experimental investigation of the steady-state flow dynamics of volume-heated boiling pool systems

  8. Mitochondrial deoxyribonucleoside triphosphate pools in thymidine kinase 2 deficiency.

    Science.gov (United States)

    Saada, Ann; Ben-Shalom, Efrat; Zyslin, Rivka; Miller, Chaya; Mandel, Hanna; Elpeleg, Orly

    2003-10-24

    Deficiency of mitochondrial thymidine kinase (TK2) is associated with mitochondrial DNA (mtDNA) depletion and manifests by severe skeletal myopathy in infancy. In order to elucidate the pathophysiology of this condition, mitochondrial deoxyribonucleoside triphosphate (dNTP) pools were determined in patients' fibroblasts. Despite normal mtDNA content and cytochrome c oxidase (COX) activity, mitochondrial dNTP pools were imbalanced. Specifically, deoxythymidine triphosphate (dTTP) content was markedly decreased, resulting in reduced dTTP:deoxycytidine triphosphate ratio. These findings underline the importance of balanced mitochondrial dNTP pools for mtDNA synthesis and may serve as the basis for future therapeutic interventions.

  9. Non-electric applications of pool-type nuclear reactors

    International Nuclear Information System (INIS)

    Adamov, E.O.; Cherkashov, Yu.M.; Romenkov, A.A.

    1997-01-01

    This paper recommends the use of pool-type light water reactors for thermal energy production. Safety and reliability of these reactors were already demonstrated to the public by the long-term operation of swimming pool research reactors. The paper presents the design experience of two projects: Apatity Underground Nuclear Heating Plant and Nuclear Sea-Water Desalination Plant. The simplicity of pool-type reactors, the ease of their manufacturing and maintenance make this type of a heat source attractive to the countries without a developed nuclear industry. (author). 6 figs, 1 tab

  10. Macroinvertebrate community assembly in pools created during peatland restoration

    International Nuclear Information System (INIS)

    Brown, Lee E.; Ramchunder, Sorain J.; Beadle, Jeannie M.; Holden, Joseph

    2016-01-01

    Many degraded ecosystems are subject to restoration attempts, providing new opportunities to unravel the processes of ecological community assembly. Restoration of previously drained northern peatlands, primarily to promote peat and carbon accumulation, has created hundreds of thousands of new open water pools. We assessed the potential benefits of this wetland restoration for aquatic biodiversity, and how communities reassemble, by comparing pool ecosystems in regions of the UK Pennines on intact (never drained) versus restored (blocked drainage-ditches) peatland. We also evaluated the conceptual idea that comparing reference ecosystems in terms of their compositional similarity to null assemblages (and thus the relative importance of stochastic versus deterministic assembly) can guide evaluations of restoration success better than analyses of community composition or diversity. Community composition data highlighted some differences in the macroinvertebrate composition of restored pools compared to undisturbed peatland pools, which could be used to suggest that alternative end-points to restoration were influenced by stochastic processes. However, widely used diversity metrics indicated no differences between undisturbed and restored pools. Novel evaluations of restoration using null models confirmed the similarity of deterministic assembly processes from the national species pool across all pools. Stochastic elements were important drivers of between-pool differences at the regional-scale but the scale of these effects was also similar across most of the pools studied. The amalgamation of assembly theory into ecosystem restoration monitoring allows us to conclude with more certainty that restoration has been successful from an ecological perspective in these systems. Evaluation of these UK findings compared to those from peatlands across Europe and North America further suggests that restoring peatland pools delivers significant benefits for aquatic fauna by

  11. Pool scrubbing and hydrodynamic experiment on jet injection regime

    Energy Technology Data Exchange (ETDEWEB)

    Peyres, V.; Espigares, M.M.; Polo, J.; Escudero, M.J.; Herranz, L.E.; Lopez, J.

    1995-07-01

    Plant analyses nave shown that pool scrubbing can play an important role in source term during PWR risk dominant sequences. An examination of boundary conditions governing fission products and aerosols transport through aqueous beds revealed that most of radioactivity is discharged into the pool under jet injection regime. This fact and the lack of experimental data under such conditions pointed the need of setting out an experimental programme which provided reliable experimental data to validate code models. In this report the major results of a pool scrubbing experimental programme carried out in PECA facility are presented. One of the major findings was that a remarkable fraction of particle absorption was not a function of the residence time of bubbles rising through the pool. Such a contribution was assumed to be associated to aerosol removal mechanism acting at the pool entrance. As a consequence. a hydrodynamic experimental plan was launched to examine the gas behaviour during the initial stages in the pool. Size and shape of gas nuclei in the pool were measured and fitted to a lognormal distribution. Particularly, size was found to be quite sensitive to inlet gas flow and at minor extent to gas composition and pool temperature. SPARC90 and BUSCA-AUG92 were used to simulate the retention tests. Whereas SPARC90 showed a pretty good agreement with experimental data, BUSCA-AUG92 results were far away from measurements in all the cases. SPARC90 consistency apparently pointed out the important role of fission products and aerosols retention at the injection zone; nonetheless, a peer examination of pool scrubbing phenomenology at the pool entrance should be carried out to test both hydrodynamic and removal models. Hence, one of the major high lights drawn from this work was the need of further research under representative severe accident conditions (i.e., saturated pools, jet injection regimes, etc.), as well as separate effect tests to validate, improve and

  12. Pool scrubbing and hydrodynamic experiment on jet injection regime

    International Nuclear Information System (INIS)

    Peyres, V.; Espigares, M.M.; Polo, J.; Escudero, M.J.; Herranz, L.E.; Lopez, J.

    1995-01-01

    Plant analyses nave shown that pool scrubbing can play an important role in source term during PWR risk dominant sequences. An examination of boundary conditions governing fission products and aerosols transport through aqueous beds revealed that most of radioactivity is discharged into the pool under jet injection regime. This fact and the lack of experimental data under such conditions pointed the need of setting out an experimental programme which provided reliable experimental data to validate code models. In this report the major results of a pool scrubbing experimental programme carried out in PECA facility are presented. One of the major findings was that a remarkable fraction of particle absorption was not a function of the residence time of bubbles rising through the pool. Such a contribution was assumed to be associated to aerosol removal mechanism acting at the pool entrance. As a consequence. a hydrodynamic experimental plan was launched to examine the gas behaviour during the initial stages in the pool. Size and shape of gas nuclei in the pool were measured and fitted to a lognormal distribution. Particularly, size was found to be quite sensitive to inlet gas flow and at minor extent to gas composition and pool temperature. SPARC90 and BUSCA-AUG92 were used to simulate the retention tests. Whereas SPARC90 showed a pretty good agreement with experimental data, BUSCA-AUG92 results were far away from measurements in all the cases. SPARC90 consistency apparently pointed out the important role of fission products and aerosols retention at the injection zone; nonetheless, a peer examination of pool scrubbing phenomenology at the pool entrance should be carried out to test both hydrodynamic and removal models. Hence, one of the major high lights drawn from this work was the need of further research under representative severe accident conditions (i.e., saturated pools, jet injection regimes, etc.), as well as separate effect tests to validate, improve and

  13. Macroinvertebrate community assembly in pools created during peatland restoration

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Lee E., E-mail: l.brown@leeds.ac.uk; Ramchunder, Sorain J.; Beadle, Jeannie M.; Holden, Joseph

    2016-11-01

    Many degraded ecosystems are subject to restoration attempts, providing new opportunities to unravel the processes of ecological community assembly. Restoration of previously drained northern peatlands, primarily to promote peat and carbon accumulation, has created hundreds of thousands of new open water pools. We assessed the potential benefits of this wetland restoration for aquatic biodiversity, and how communities reassemble, by comparing pool ecosystems in regions of the UK Pennines on intact (never drained) versus restored (blocked drainage-ditches) peatland. We also evaluated the conceptual idea that comparing reference ecosystems in terms of their compositional similarity to null assemblages (and thus the relative importance of stochastic versus deterministic assembly) can guide evaluations of restoration success better than analyses of community composition or diversity. Community composition data highlighted some differences in the macroinvertebrate composition of restored pools compared to undisturbed peatland pools, which could be used to suggest that alternative end-points to restoration were influenced by stochastic processes. However, widely used diversity metrics indicated no differences between undisturbed and restored pools. Novel evaluations of restoration using null models confirmed the similarity of deterministic assembly processes from the national species pool across all pools. Stochastic elements were important drivers of between-pool differences at the regional-scale but the scale of these effects was also similar across most of the pools studied. The amalgamation of assembly theory into ecosystem restoration monitoring allows us to conclude with more certainty that restoration has been successful from an ecological perspective in these systems. Evaluation of these UK findings compared to those from peatlands across Europe and North America further suggests that restoring peatland pools delivers significant benefits for aquatic fauna by

  14. Modeling of the Temperature Field Recovery in the Oil Pool

    Science.gov (United States)

    Khabibullin, I. L.; Davtetbaev, A. Ya.; Mar'in, D. F.; Khisamov, A. A.

    2018-05-01

    This paper considers the problem on mathematical modeling of the temperature field recovery in the oil pool upon termination of injection of water into the pool. The problem is broken down into two stages: injection of water and temperature and pressure recovery upon termination of injection. A review of the existing mathematical models is presented, analytical solutions for a number of cases have been constructed, and a comparison of the analytical solutions of different models has been made. In the general form, the expression has been obtained that permits determining the temperature change in the oil pool upon termination of injection of water (recovery of the temperature field).

  15. Core-concrete molten pool dynamics and interfacial heat transfer

    International Nuclear Information System (INIS)

    Benjamin, A.S.

    1980-01-01

    Theoretical models are derived for the heat transfer from molten oxide pools to an underlying concrete surface and from molten steel pools to a general concrete containment. To accomplish this, two separate effects models are first developed, one emphasizing the vigorous agitation of the molten pool by gases evolving from the concrete and the other considering the insulating effect of a slag layer produced by concrete melting. The resulting algebraic expressions, combined into a general core-concrete heat transfer representation, are shown to provide very good agreement with experiments involving molten steel pours into concrete crucibles

  16. Screening for residual disease in pediatric burkitt lymphoma using consensus primer pools.

    Science.gov (United States)

    Agsalda, Melissa; Kusao, Ian; Troelstrup, David; Shiramizu, Bruce

    2009-01-01

    Assessing molecular persistent or minimal residual disease (PD/MRD) in childhood Burkitt lymphoma (BL) is challenging because access to original tumor is usually needed to design patient-specific primers (PSPs). Because BL is characterized by rearranged immunoglobulin heavy chain (IgV(H)) genes, IgV(H) primer pools from IgV(H1)-IgV(H7) regions were tested to detect PD/MRD, thus eliminating the need for original tumor. The focus of the current study was to assess the feasibility of using IgV(H) primer pools to detect disease in clinical specimens. Fourteen children diagnosed with B-NHL had follow-up repository specimens available to assess PD/MRD. Of the 14 patients, 12 were PD/MRD negative after 2 months of therapy and remained in remission at the end of therapy; 2/14 patients were PD/MRD positive at 2-3 months and later relapsed. PSP-based assays from these 14 patients showed 100% concordance with the current assay. This feasibility study warrants further investigation to assess PD/MRD using IgV(H) primer pools, which could have clinical significance as a real-time assessment tool to monitor pediatric BL and possibly other B-cell non-Hodgkin lymphoma therapy.

  17. Screening for Residual Disease in Pediatric Burkitt Lymphoma Using Consensus Primer Pools

    Directory of Open Access Journals (Sweden)

    Melissa Agsalda

    2009-01-01

    Full Text Available Assessing molecular persistent or minimal residual disease (PD/MRD in childhood Burkitt lymphoma (BL is challenging because access to original tumor is usually needed to design patient-specific primers (PSPs. Because BL is characterized by rearranged immunoglobulin heavy chain (IgVH genes, IgVH primer pools from IgVH1–IgVH7 regions were tested to detect PD/MRD, thus eliminating the need for original tumor. The focus of the current study was to assess the feasibility of using IgVH primer pools to detect disease in clinical specimens. Fourteen children diagnosed with B-NHL had follow-up repository specimens available to assess PD/MRD. Of the 14 patients, 12 were PD/MRD negative after 2 months of therapy and remained in remission at the end of therapy; 2/14 patients were PD/MRD positive at 2-3 months and later relapsed. PSP-based assays from these 14 patients showed 100% concordance with the current assay. This feasibility study warrants further investigation to assess PD/MRD using IgVH primer pools, which could have clinical significance as a real-time assessment tool to monitor pediatric BL and possibly other B-cell non-Hodgkin lymphoma therapy.

  18. Retail Demand Response in Southwest Power Pool

    Energy Technology Data Exchange (ETDEWEB)

    Bharvirkar, Ranjit; Heffner, Grayson; Goldman, Charles

    2009-01-30

    In 2007, the Southwest Power Pool (SPP) formed the Customer Response Task Force (CRTF) to identify barriers to deploying demand response (DR) resources in wholesale markets and develop policies to overcome these barriers. One of the initiatives of this Task Force was to develop more detailed information on existing retail DR programs and dynamic pricing tariffs, program rules, and utility operating practices. This report describes the results of a comprehensive survey conducted by LBNL in support of the Customer Response Task Force and discusses policy implications for integrating legacy retail DR programs and dynamic pricing tariffs into wholesale markets in the SPP region. LBNL conducted a detailed survey of existing DR programs and dynamic pricing tariffs administered by SPP's member utilities. Survey respondents were asked to provide information on advance notice requirements to customers, operational triggers used to call events (e.g. system emergencies, market conditions, local emergencies), use of these DR resources to meet planning reserves requirements, DR resource availability (e.g. seasonal, annual), participant incentive structures, and monitoring and verification (M&V) protocols. Nearly all of the 30 load-serving entities in SPP responded to the survey. Of this group, fourteen SPP member utilities administer 36 DR programs, five dynamic pricing tariffs, and six voluntary customer response initiatives. These existing DR programs and dynamic pricing tariffs have a peak demand reduction potential of 1,552 MW. Other major findings of this study are: o About 81percent of available DR is from interruptible rate tariffs offered to large commercial and industrial customers, while direct load control (DLC) programs account for ~;;14percent. o Arkansas accounts for ~;;50percent of the DR resources in the SPP footprint; these DR resources are primarily managed by cooperatives. o Publicly-owned cooperatives accounted for 54percent of the existing DR resources

  19. Pool Onistarist kuulub kütusefirma omanikele / Sirje Niitra

    Index Scriptorium Estoniae

    Niitra, Sirje, 1948-

    2001-01-01

    Alkoholikontserni Onistar kiiret tõusu Eesti alkoholiturul toetavad Vene rahvusest ärimeestele kuuluvate kütusefirmade Infast Oil ja Saurix Petroleum omanikud, kellele kuulub pool Eesti viinaturu liidri aktsiatest

  20. Environmental assessment, K Pool fish rearing, Hanford Site, Richland, Washington

    International Nuclear Information System (INIS)

    1996-12-01

    The US Department of Energy (DOE) has a need to respond to a request to lease facilities at the Hanford Site 100-KE and 100-KW filter plant pools (K Pools) for fish rearing activities. These fish rearing activities would be: (1) business ventures with public and private funds and (2) long-term enhancement and supplementation programs for game fish populations in the Columbia River Basin. The proposed action is to enter into a use permit or lease agreement with the YIN or other parties who would rear fish in the 100-K Area Pools. The proposed action would include necessary piping, pump, and electrical upgrades of the facility; cleaning and preparation of the pools; water withdrawal from the Columbia River, and any necessary water or wastewater treatment; and introduction, rearing and release of fish. Future commercial operations may be included

  1. 41 CFR 101-25.109-2 - Equipment pools.

    Science.gov (United States)

    2010-07-01

    ... number and location of equipment pools, consideration shall be given to economy of operation, mobility of... available for sharing or loan. Information concerning the availability of this equipment can be maintained...

  2. Open Architecture, Inventory Pooling and Spare Maintenance Assets

    National Research Council Canada - National Science Library

    Ferrer, Geraldo

    2007-01-01

    .... This article focuses on the benefits provided by pooling together the inventory necessary to meet the demand of many users into a small number of storage sites with reduced product variety obtained...

  3. Investigation to radioactive contamination of pool water in IMEF

    International Nuclear Information System (INIS)

    Song, Ung Sup; Jung, Yang Hong; Lee, J. H.; Lee, H. K.

    2003-06-01

    The pool (3x6x10) in irradiated materials examination facility is usually used for the purpose of taking the specimen out of cask loaded into the pool, and carrying in/out the specimen to/ from the hot cell. Always, it must be cared for the water into the pool to be fine condition because all operation are worked with the naked eye during taking an irradiated materials out of the cask and plunging them in the bucket-elevator. In the aspects of the radioactive remained substances in the water must be controlled so that the amount of substances to be lower than the standard amount prescribed by RCA Korea Activity in a part of radioactive contamination control. In consequence, an expertness of status and a practical use of skill make possible the prevention of radioactive material's diffusion or the radioactive contamination of pool water and safety work

  4. Allegheny County Public Swimming Pool, Hot Tub, and Spa Inspections

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Public swimming pool, hot tub, and spa facilities are licensed and inspected once each year to assure proper water quality, sanitation, lifeguard coverage and...

  5. 17 CFR 229.1111 - (Item 1111) Pool assets.

    Science.gov (United States)

    2010-04-01

    ... information for the asset pool, including statistical information regarding delinquencies and losses. (d.... Present statistical information in tabular or graphical format, if such presentation will aid understanding. Present statistical information in appropriate distributional groups or incremental ranges in...

  6. Equilibrium blood pool scanning in the evaluation of hemophilic arthropathy

    International Nuclear Information System (INIS)

    Spies, S.; Green, D.; Rana, N.A.; Milgram, J.W.; Mintzer, R.

    1978-01-01

    Arthropathy was evaluated in six patients with severe hemophilia (factor VIII<1%) using the technique of blood pool scanning. Employing an in vivo method for erythrocyte labelling with technetium-99m, a dynamic perfusion sequence was obtained using a scintillation camera over the joint(s) to be examined. Subsequently, equilibrium blood pool images of the joints were obtained to determine regional blood volume. In young patients with repeated episodes of acute hemarthrosis, increased vascularity and increased blood pool in the affected joints were demonstrated. In older patients with fixed, contracted joints and degenerative arthropathy, vascularity and regional blood volume were not abnormal. Blood pool scanning is a safe, non-invasive technique that augments the clinical and radiographic examination of the joints. The method is helpful in distinguishing acute joint bleeding from chronic synovitis and arthritis, and may prove useful in selecting patients for synovectomy. (author)

  7. LCG Persistency Framework (POOL, CORAL, COOL) - Status and Outlook

    CERN Multimedia

    CERN. Geneva

    2012-01-01

    The LCG Persistency Framework consists of three software packages (POOL, CORAL and COOL) that address the data access requirements of the LHC experiments in several different areas. The project is the result of the collaboration between the CERN IT Department and the three experiments (ATLAS, CMS and LHCb) that are using some or all of the Persistency Framework components to access their data. The POOL package is a hybrid technology store for C++ objects, using a mixture of streaming and relational technologies to implement both object persistency and object metadata catalogs and collections. POOL provides generic components that can be used by the experiments to store both their event data and their conditions data. The CORAL package is an abstraction layer with an SQL-free API for accessing data stored using relational database technologies. It is used directly by experiment-specific applications and internally by both COOL and POOL. The COOL package provides specific software components and tools for the h...

  8. Environmental assessment, K Pool fish rearing, Hanford Site, Richland, Washington

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    The US Department of Energy (DOE) has a need to respond to a request to lease facilities at the Hanford Site 100-KE and 100-KW filter plant pools (K Pools) for fish rearing activities. These fish rearing activities would be: (1) business ventures with public and private funds and (2) long-term enhancement and supplementation programs for game fish populations in the Columbia River Basin. The proposed action is to enter into a use permit or lease agreement with the YIN or other parties who would rear fish in the 100-K Area Pools. The proposed action would include necessary piping, pump, and electrical upgrades of the facility; cleaning and preparation of the pools; water withdrawal from the Columbia River, and any necessary water or wastewater treatment; and introduction, rearing and release of fish. Future commercial operations may be included.

  9. Condensation pool experiments with steam using DN200 blowdown pipe

    International Nuclear Information System (INIS)

    Laine, J.; Puustinen, M.

    2005-08-01

    This report summarizes the results of the condensation pool experiments with steam using a DN200 blowdown pipe. Altogether five experiment series, each consisting of several steam blows, were carried out in December 2004 with a scaled-down test facility designed and constructed at Lappeenranta University of Technology. The main purpose of the experiments was to increase the understanding of different phenomena in the condensation pool during steam discharge. (au)

  10. Optimization of spent fuel pool weir gate driving mechanism

    Science.gov (United States)

    Liu, Chao; Du, Lin; Tao, Xinlei; Wang, Shijie; Shang, Ertao; Yu, Jianjiang

    2018-04-01

    Spent fuel pool is crucial facility for fuel storage and nuclear safety, and the spent fuel pool weir gate is the key related equipment. In order to achieve a goal of more efficient driving force transfer, loading during the opening/closing process is analyzed and an optimized calculation method for dimensions of driving mechanism is proposed. The result of optimizing example shows that the method can be applied to weir gates' design with similar driving mechanism.

  11. CHARACTERISTICS OFBENTHIC FISH COMMUNITY OF DNIEPER STORAGE POOL LITTORAL ZONE

    Directory of Open Access Journals (Sweden)

    Novitskiy R. A.

    2011-11-01

    Full Text Available Specific composition of bull-calves is analyzed together with their quantitative and quality parameters in the littoral zone of the Dnepr storage pool. The structural-functional features of organization of littoral communities of bull-calves were studied. The patterns of spatial distribution of Bull-calf (Gobiidae representatives were analyzed for the storage pool; their role in the littoral fish communities was clarified.

  12. Research progresses and future directions on pool boiling heat transfer

    OpenAIRE

    M. Kumar; V. Bhutani; P. Khatak

    2015-01-01

    This paper reviews the previous work carried on pool boiling heat transfer during heating of various liquids and commodities categorized as refrigerants and dielectric fluids, pure liquids, nanofluids, hydrocarbons and additive mixtures, as well as natural and synthetic colloidal solutions. Nucleate pool boiling is an efficient and effective method of boiling because high heat fluxes are possible with moderate temperature differences. It is characterized by the growth of bubbles on a heated s...

  13. Analysis of natural convection in volumetrically-heated melt pools

    International Nuclear Information System (INIS)

    Sehgal, B.R.; Dinh, T.N.; Nourgaliev, R.R.

    1996-12-01

    Results of series of studies on natural convection heat transfer in decay-heated core melt pools which form in a reactor lower plenum during the progression of a core meltdown accident are described. The emphasis is on modelling and prediction of turbulent heat transfer characteristics of natural convection in a liquid pool with an internal energy source. Methods of computational fluid dynamics, including direct numerical simulation, were applied for investigation

  14. Cryptosporidium and Giardia in Swimming Pools, Atlanta, Georgia

    Centers for Disease Control (CDC) Podcasts

    In this podcast, Dan Rutz speaks with Dr. Joan Shields, a guest researcher with the Healthy Swimming Program at CDC, about an article in June 2008 issue of Emerging Infectious Diseases reporting on the results of a test of swimming pools in the greater Atlanta, Georgia area. Dr. Shields tested 160 pools in metro Atlanta last year for Cryptosporidium and Giardia. These germs cause most recreational water associated outbreaks.

  15. A swimming pool array for ultra high energy showers

    Science.gov (United States)

    Yodh, Gaurang B.; Shoup, Anthony; Barwick, Steve; Goodman, Jordan A.

    1992-11-01

    A very preliminary design concept for an array using water Cherenkov counters, built out of commercially available backyard swimming pools, to sample the electromagnetic and muonic components of ultra high energy showers at large lateral distances is presented. The expected performance of the pools is estimated using the observed lateral distributions by scintillator and water Cherenkov arrays at energies above 1019 eV and simulations.

  16. Lip and tooth injuries at public swimming pools in Austria.

    Science.gov (United States)

    Lechner, Katharina; Connert, Thomas; Kühl, Sebastian; Filippi, Andreas

    2017-06-01

    There is an increased risk of orofacial injuries in swimming pool facilities. Nevertheless, only a few studies have addressed this issue. The aim of this study was to identify the frequency of lip and tooth injuries at public swimming pools in Austria. A further aim was to examine which gender and age groups were affected, where and why these injuries occurred, and whether pool attendants had sufficient knowledge of dental first-aid measures. A total of 764 pool attendants in Austria were contacted by telephone and 689 participated in the study (90.2%). The attendants were interviewed retrospectively about accident occurrences in 2014 by a standardized questionnaire. Responses to the provision of first aid and choice of storage medium for avulsed teeth were subsequently evaluated. The frequency of lip injuries was 19.0%, and tooth injuries were 11.3%. Male bathers (P < .05) and children under 12 years (P < .001) most frequently suffered injuries. The waterslide was the most common accident site. The most common cause of lip injuries was slipping on wet surfaces (39.0%), and for tooth injuries it was collisions with other persons or objects (each 28.1%). The pool attendants' responses were predominantly good or sufficient on first aid, with the exception of what storage medium to choose. Tooth rescue boxes were available in only 8.6% of all pool facilities. Orofacial injuries are a frequently occurring problem in swimming pool facilities. The pool attendants' knowledge on first-aid care of tooth injuries could still be improved. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  17. A hierarchic sustainability dashboard to evaluate logistics pooling

    OpenAIRE

    Morana, Joëlle; Gonzalez-Feliu, Jesus

    2014-01-01

    Logistics pooling is now a major challenge in supply chain management, though it remains a little known activity in which the different actors involved use different approaches whose objectives are not always the same and with sometimes conflicting standpoints. This purpose of this article is to define, on the basis of a detailed analysis of the literature, a grid for interpreting and a dashboard for evaluating the sustainable performance of pooled delivery systems. Firstly, an analysis of th...

  18. Analysis of natural convection in volumetrically-heated melt pools

    Energy Technology Data Exchange (ETDEWEB)

    Sehgal, B.R.; Dinh, T.N.; Nourgaliev, R.R. [Royal Inst. of Tech., Stockholm (Sweden). Div. of Nuclear Power Safety

    1996-12-01

    Results of series of studies on natural convection heat transfer in decay-heated core melt pools which form in a reactor lower plenum during the progression of a core meltdown accident are described. The emphasis is on modelling and prediction of turbulent heat transfer characteristics of natural convection in a liquid pool with an internal energy source. Methods of computational fluid dynamics, including direct numerical simulation, were applied for investigation. Refs, figs, tabs.

  19. Competition and power pooling in the electricity industry

    International Nuclear Information System (INIS)

    Jasinski, P.; Yarrow, G.; Fusaro, P.

    1995-01-01

    Two contributions to the debate on power pooling and competition in the electricity supply industry are presented. In the first, the situation in England and Wales where power pooling was introduced shortly before privatization is analysed. Pooling has existed for many years in the USA but as an inter-generator arrangement designed to optimize production and costs. The British system sought in addition to establish a spot market in which bulk buyers as well as bulk suppliers could participate. There are however obvious weaknesses in the pool as a competitive market and it is argued that a combination of pooling arrangements and longer-term bilateral supply contracts are more likely to offer the prospect of workably competitive and efficient outcomes. The second contribution discusses how competitive markets will evolve in the USA under the new regime of less onerous regulation heralded by the Energy Policy Act if 1992 and the emergence of electricity trading markets which followed. In particular, the proposal by the California Public Utility commission favouring a mandatory Poolco is examined. Poolco would operate the transmission grid and dispatch generating plants while the utilities would retain ownership of transmission facilities and be required to supply the pool. This is seen as representing only partial deregulation and not to be consistent with moves to further competition and encourage free markets. (U.K.)

  20. Test Area North Pool Stabilization Project: Environmental assessment

    International Nuclear Information System (INIS)

    1996-05-01

    The Test Area North (TAN) Pool is located within the fenced TAN facility boundaries on the Idaho National Engineering Laboratory (INEL). The TAN pool stores 344 canisters of core debris from the March, 1979, Three Mile Island (TMI) Unit 2 reactor accident; fuel assemblies from Loss-of-Fluid Tests (LOFT); and Government-owned commercial fuel rods and assemblies. The LOFT and government owned commercial fuel rods and assemblies are hereafter referred to collectively as open-quotes commercial fuelsclose quotes except where distinction between the two is important to the analysis. DOE proposes to remove the canisters of TMI core debris and commercial fuels from the TAN Pool and transfer them to the Idaho Chemical Processing Plant (ICPP) for interim dry storage until an alternate storage location other than at the INEL, or a permanent federal spent nuclear fuel (SNF) repository is available. The TAN Pool would be drained and placed in an industrially and radiologically safe condition for refurbishment or eventual decommissioning. This environmental assessment (EA) identifies and evaluates environmental impacts associated with (1) constructing an Interim Storage System (ISS) at ICPP; (2) removing the TMI and commercial fuels from the pool and transporting them to ICPP for placement in an ISS, and (3) draining and stabilizing the TAN Pool. Miscellaneous hardware would be removed and decontaminated or disposed of in the INEL Radioactive Waste Management Complex (RWMC). This EA also describes the environmental consequences of the no action alternative

  1. LNG pool fire simulation for domino effect analysis

    International Nuclear Information System (INIS)

    Masum Jujuly, Muhammad; Rahman, Aziz; Ahmed, Salim; Khan, Faisal

    2015-01-01

    A three-dimensional computational fluid dynamics (CFD) simulation of liquefied natural gas (LNG) pool fire has been performed using ANSYS CFX-14. The CFD model solves the fundamental governing equations of fluid dynamics, namely, the continuity, momentum and energy equations. Several built-in sub-models are used to capture the characteristics of pool fire. The Reynolds-averaged Navier–Stokes (RANS) equation for turbulence and the eddy-dissipation model for non-premixed combustion are used. For thermal radiation, the Monte Carlo (MC) radiation model is used with the Magnussen soot model. The CFD results are compared with a set of experimental data for validation; the results are consistent with experimental data. CFD results show that the wind speed has significant contribution on the behavior of pool fire and its domino effects. The radiation contours are also obtained from CFD post processing, which can be applied for risk analysis. The outcome of this study will be helpful for better understanding of the domino effects of pool fire in complex geometrical settings of process industries. - Highlights: • Simulation of pool fire using computational fluid dynamics (CFD) model. • Integration of CFD based pool fire model with domino effect. • Application of the integrated CFD based domino effect analysis

  2. Seasonal variation in Chironomid emergence from coastal pools

    Directory of Open Access Journals (Sweden)

    Alexander T. Egan

    2015-07-01

    Full Text Available Understanding the phenology of emergences can be useful in determining seasonal chironomid life cycle patterns, which are often influenced by ice cover and temperature in cold climates. Lake Superior is the largest lake in North America and with a mean surface temperature of 3.9 °C influences regional climate. Coastal pools at Isle Royale, a wilderness archipelago in the northern part of the lake, occur in dense patches on low-gradient volcanic bedrock between the lakeshore and forest, creating variable microhabitats for Chironomidae. Four sites were sampled monthly from April to October, 2010. Surface-floating pupal exuviae were collected from a series of pools in two zones: a lower zone near the lake influenced by wave splash, and an upper zone near the forest and influenced by upland runoff. We used Jaccard’s and Whittaker’s diversity indexes to test community similarity across months. Temperature loggers in pools collected hourly readings for most of the study. Assemblage emergences were stable in upper pools, with significant similarity across late spring and summer months. Assemblages were seasonally variable in lower pools, with significant dissimilarity across spring, summer, and fall months. Few species in either zone were unique to spring or fall months. However, many summer species in the splash zone had a narrow emergence period occurring during calm weather following distinct increases in mean water temperature. Regardless of input of cold lake water to the lower zone, pools from both zones generally had corresponding temperature trends.

  3. Review of steam jet condensation in a water pool

    International Nuclear Information System (INIS)

    Kim, Y. S.; Song, C. H.; Park, C. K.; Kang, H. S.; Jeon, H. G.; Yoon, Y. J.

    2002-01-01

    In the advanced nuclear power plants including APR1400, the SDVS is adopted to increase the plant safety using the concept of feed-and-bleed operation. In the case of the TLOFW, the POSRV located at the top of the pressurizer is expected to open due to the pressurization of the reactor coolant system and discharges steam and/or water mixture into the water pool, where the mixture is condensed. During the condensation of the mixture, thermal-hydraulic loads such as pressure and temperature variations are induced to the pool structure. For the pool structure design, such thermal-hydraulic aspects should be considered. Understanding the phenomena of the submerged steam jet condensation in a water pool is helpful for system designers to design proper pool structure, sparger, and supports etc. This paper reviews and evaluates the steam jet condensation in a water pool on the physical phenomena of the steam condensation including condensation regime map, heat transfer coefficient, steam plume, steam jet condensation load, and steam jet induced flow

  4. Natural convection heat transfer in the molten metal pool

    International Nuclear Information System (INIS)

    Park, R.J.; Kim, S.B.; Kim, H.D.; Choi, S.M.

    1997-01-01

    Analytical studies using the FLOW-3D computer program have been performed on natural convection heat transfer of a high density molten metal pool, in order to evaluate the coolability of the corium pool. The FLOW-3D results on the temperature distribution and the heat transfer rate in the molten metal pool region have been compared and evaluated with the experimental data. The FLOW-3D results have shown that the developed natural convection flow contributes to the solidified crust formation of the high density molten metal pool. The present FLOW-3D results, on the relationship between the Nusselt number and the Rayleigh number in the molten metal pool region, are more similar to the calculated results of Globe and Dropkin's correlation than any others. The natural convection heat transfer in the low aspect ratio case is more substantial than that in the high aspect ratio case. The FLOW-3D results, on the temperature profile and on the heat transfer rate in the molten metal pool region, are very similar to the experimental data. The heat transfer rate of the internal heat generation case is higher than that of the bottom heating case at the same heat supply condition. (author)

  5. Detection of 41Ar diffusion from a TRIGA pool

    International Nuclear Information System (INIS)

    Foss, Scott; Nelson, George

    1990-01-01

    Argon-41 levels in very low concentrations in the reactor room air can be inferred from the rate of escape from the pool water surface. The rate of Argon-41 diffusion from pool water to room air was determined by the measurement of the activity buildup in containers of air in contact with the pool surface. The Argon-41 concentration in each container was measured by gamma-ray spectrometry with a calibrated GeLi detector. At 100 KW power with the water temperature at 10 deg. Celsius the total release of Argon-41 was determined to be 3.5e10±15% atoms for 80 minutes of operation. The corresponding activity released from the pool was 98.6 microcuries, while the total activity produced in the pool was 4900 microcuries. The diffusion coefficient of Argon-41 from the pool water surface to the air at this temperature was measured to be 3.14e-14 sec-cm 2 . (author)

  6. Structural analysis of the reactor pool for the RRRP

    International Nuclear Information System (INIS)

    Alberro, J.G.; Abbate, A.D.

    2005-01-01

    The purpose of the present document is to describe the structural design of the Reactor Pool relevant to the RRRP (Replacement Research Reactor Project) for the Australian Nuclear Science and Technology Organisation. The structural analysis required coordinated design, engineering, analysis, and fabrication efforts. The pool has been designed, manufactured, and inspected following as guideline the ASME Boiler and Pressure Vessel Code, which defines the requirements for the pool to withstand hydrostatic and mechanical forces, ensuring its integrity throughout its lifetime. Standard off-the-shelf finite element programs (Nastran and Ansys codes) were used to evaluate the pool and further qualify the design and its construction. Both global and local effect analyses were carried out. The global analysis covers the structural integrity of the pool wall (6 mm thick) considering the different load states acting on it, namely hydrostatic pressure, thermal expansion, and seismic event. The local analysis evaluates the structural behaviour of the pool at specific points resulting from the interaction among components. It is confirmed that maximum stresses and displacements fall below the allowable values required by the ASME Boiler and Pressure Vessel Code. The water pressure analysis was validated by means of a hydrostatic test. (authors)

  7. 76 FR 64085 - Post-2014 Resource Pool-Loveland Area Projects, Final Power Allocation

    Science.gov (United States)

    2011-10-17

    ... Resource Pool; Loveland Area Projects, General Eligibility Criteria. The resource pool for capacity and... transmission in Kansas. Final allocation of the Post-2014 Resource Pool; Loveland Area Projects, is contingent...

  8. Genomic and Transcriptomic Evidence for Carbohydrate Consumption Among Microorganisms in a Cold Seep Brine Pool

    Directory of Open Access Journals (Sweden)

    Weipeng Zhang

    2016-11-01

    Full Text Available The detailed lifestyle of microorganisms in deep-sea brine environments remains largely unexplored. Using a carefully calibrated genome binning approach, we reconstructed partial to nearly-complete genomes of 51 microorganisms in biofilms from the Thuwal cold seep brine pool of the Red Sea. The recovered metagenome-assembled genomes (MAGs belong to six different phyla: Actinobacteria, Proteobacteria, Candidatus Cloacimonetes, Candidatus Marinimicrobia, Bathyarchaeota and Thaumarchaeota. By comparison with close relatives of these microorganisms, we identified a number of unique genes associated with organic carbon metabolism and energy generation. These genes included various glycoside hydrolases, nitrate and sulfate reductases, putative bacterial microcompartment biosynthetic clusters (BMC, and F420H2 dehydrogenases. Phylogenetic analysis suggested that the acquisition of these genes probably occurred through horizontal gene transfer (HGT. Metatranscriptomics illustrated that glycoside hydrolases are among the most highly expressed genes. Our results suggest that the microbial inhabitants are well adapted to this brine environment, and anaerobic carbohydrate consumption mediated by glycoside hydrolases and electron transport systems (ETSs is a dominant process performed by microorganisms from various phyla within this ecosystem.

  9. Genomic and Transcriptomic Evidence for Carbohydrate Consumption among Microorganisms in a Cold Seep Brine Pool

    KAUST Repository

    Zhang, Weipeng

    2016-11-15

    The detailed lifestyle of microorganisms in deep-sea brine environments remains largely unexplored. Using a carefully calibrated genome binning approach, we reconstructed partial to nearly-complete genomes of 51 microorganisms in biofilms from the Thuwal cold seep brine pool of the Red Sea. The recovered metagenome-assembled genomes (MAGs) belong to six different phyla: Actinobacteria, Proteobacteria, Candidatus Cloacimonetes, Candidatus Marinimicrobia, Bathyarchaeota, and Thaumarchaeota. By comparison with close relatives of these microorganisms, we identified a number of unique genes associated with organic carbon metabolism and energy generation. These genes included various glycoside hydrolases, nitrate and sulfate reductases, putative bacterial microcompartment biosynthetic clusters (BMC), and F420H2 dehydrogenases. Phylogenetic analysis suggested that the acquisition of these genes probably occurred through horizontal gene transfer (HGT). Metatranscriptomics illustrated that glycoside hydrolases are among the most highly expressed genes. Our results suggest that the microbial inhabitants are well adapted to this brine environment, and anaerobic carbohydrate consumption mediated by glycoside hydrolases and electron transport systems (ETSs) is a dominant process performed by microorganisms from various phyla within this ecosystem.

  10. Mathematical modelling and simulation of the thermal performance of a solar heated indoor swimming pool

    OpenAIRE

    Mančić Marko V.; Živković Dragoljub S.; Milosavljević Peđa M.; Todorović Milena N.

    2014-01-01

    Buildings with indoor swimming pools have a large energy footprint. The source of major energy loss is the swimming pool hall where air humidity is increased by evaporation from the pool water surface. This increases energy consumption for heating and ventilation of the pool hall, fresh water supply loss and heat demand for pool water heating. In this paper, a mathematical model of the swimming pool was made to assess energy demands of an indoor swimming po...

  11. Pooled versus separate measurements of tree-ring stable isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Dorado Linan, Isabel, E-mail: isabel@gfz-potsdam.de [Universitat de Barcelona, Departament d' Ecologia, Diagonal 645, 08028, Barcelona (Spain); German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany); Gutierrez, Emilia, E-mail: emgutierrez@ub.edu [Universitat de Barcelona, Departament d' Ecologia, Diagonal 645, 08028, Barcelona (Spain); Helle, Gerhard, E-mail: ghelle@gfz-potsdam.de [German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany); Heinrich, Ingo, E-mail: heinrich@gfz-potsdam.de [German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany); Andreu-Hayles, Laia, E-mail: laiandreu@ub.edu [Universitat de Barcelona, Departament d' Ecologia, Diagonal 645, 08028, Barcelona (Spain); Tree-Ring Laboratory, Lamont-Doherty Earth Observatory of Columbia University, Palisades NY (United States); Planells, Octavi, E-mail: leocarpus@hotmail.com [Universitat de Barcelona, Departament d' Ecologia, Diagonal 645, 08028, Barcelona (Spain); Leuenberger, Markus, E-mail: leuenberger@climate.unibe.ch [Climate and Environmental Physics, Physics Institute, University of Bern, Sidlerstrasse 5, 3012 Bern (Switzerland); Oeschger Centre of Climate Change Research, University of Bern, Zaehringerstrasse 25, 3012 Bern (Switzerland); Buerger, Carmen, E-mail: buerger@gfz-potsdam.de [German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany); Schleser, Gerhard, E-mail: schleser@gfz-potsdam.de [German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany)

    2011-05-01

    {delta}{sup 13}C and {delta}{sup 18}O of tree rings contain time integrated information about the environmental conditions weighted by seasonal growth dynamics and are well established as sources of palaeoclimatic and ecophysiological data. Annually resolved isotope chronologies are frequently produced by pooling dated growth rings from several trees prior to the isotopic analyses. This procedure has the advantage of saving time and resources, but precludes from defining the isotopic error or statistical uncertainty related to the inter-tree variability. Up to now only a few studies have compared isotope series from pooled tree rings with isotopic measurements from individual trees. We tested whether or not the {delta}{sup 13}C and the {delta}{sup 18}O chronologies derived from pooled and from individual tree rings display significant differences at two locations from the Iberian Peninsula to assess advantages and constraints of both methodologies. The comparisons along the period 1900-2003 reveal a good agreement between pooled chronologies and the two mean master series which were created by averaging raw individual values (Mean) or by generating a mass calibrated mean (MassC). In most of the cases, pooled chronologies show high synchronicity with averaged individual samples at interannual scale but some differences also show up especially when comparing {delta}{sup 18}O decadal to multi-decadal variations. Moreover, differences in the first order autocorrelation among individuals may be obscured by pooling strategies. The lack of replication of pooled chronologies prevents detection of a bias due to a higher mass contribution of one sample but uncertainties associated with the analytical process itself, as sample inhomogeneity, seems to account for the observed differences. - Research Highlights: {yields} Pooled {delta}{sup 13}C and {delta}{sup 18}O chronologies are expected to be similar to the mean. {yields} Empirical pooled chronologies {delta}{sup 13}C and

  12. Pooled versus separate measurements of tree-ring stable isotopes

    International Nuclear Information System (INIS)

    Dorado Linan, Isabel; Gutierrez, Emilia; Helle, Gerhard; Heinrich, Ingo; Andreu-Hayles, Laia; Planells, Octavi; Leuenberger, Markus; Buerger, Carmen; Schleser, Gerhard

    2011-01-01

    δ 13 C and δ 18 O of tree rings contain time integrated information about the environmental conditions weighted by seasonal growth dynamics and are well established as sources of palaeoclimatic and ecophysiological data. Annually resolved isotope chronologies are frequently produced by pooling dated growth rings from several trees prior to the isotopic analyses. This procedure has the advantage of saving time and resources, but precludes from defining the isotopic error or statistical uncertainty related to the inter-tree variability. Up to now only a few studies have compared isotope series from pooled tree rings with isotopic measurements from individual trees. We tested whether or not the δ 13 C and the δ 18 O chronologies derived from pooled and from individual tree rings display significant differences at two locations from the Iberian Peninsula to assess advantages and constraints of both methodologies. The comparisons along the period 1900-2003 reveal a good agreement between pooled chronologies and the two mean master series which were created by averaging raw individual values (Mean) or by generating a mass calibrated mean (MassC). In most of the cases, pooled chronologies show high synchronicity with averaged individual samples at interannual scale but some differences also show up especially when comparing δ 18 O decadal to multi-decadal variations. Moreover, differences in the first order autocorrelation among individuals may be obscured by pooling strategies. The lack of replication of pooled chronologies prevents detection of a bias due to a higher mass contribution of one sample but uncertainties associated with the analytical process itself, as sample inhomogeneity, seems to account for the observed differences. - Research Highlights: → Pooled δ 13 C and δ 18 O chronologies are expected to be similar to the mean. → Empirical pooled chronologies δ 13 C and δ 18 O and the mean show a high synchronicity. → Pooled chronologies differ

  13. Convective Cold Pool Structure and Boundary Layer Recovery in DYNAMO

    Science.gov (United States)

    Savarin, A.; Chen, S. S.; Kerns, B. W.; Lee, C.; Jorgensen, D. P.

    2012-12-01

    One of the key factors controlling convective cloud systems in the Madden-Julian Oscillation (MJO) over the tropical Indian Ocean is the property of the atmospheric boundary layer. Convective downdrafts and precipitation from the cloud systems produce cold pools in the boundary layer, which can inhibit subsequent development of convection. The recovery time is the time it takes for the boundary layer to return to pre convective conditions. It may affect the variability of the convection on various time scales during the initiation of MJO. This study examines the convective cold pool structure and boundary layer recovery using the NOAA WP-3D aircraft observations, include the flight-level, Doppler radar, and GPS dropsonde data, collected during the Dynamics of MJO (DYNAMO) field campaign from November-December 2011. The depth and strength of convective cold pools are defined by the negative buoyancy, which can be computed from the dropsonde data. Convective downdraft can be affected by environmental water vapor due to entrainment. Mid-level dry air observed during the convectively suppressed phase of MJO seems to enhance convective downdraft, making the cold pools stronger and deeper. Recovery of the cold pools in the boundary layer is determined by the strength and depth of the cold pools and also the air-sea heat and moisture fluxes. Given that the water vapor and surface winds are distinct for the convectively active and suppressed phases of MJO over the Indian Ocean, the aircraft data are stratified by the two different large-scale regimes of MJO. Preliminary results show that the strength and depth of the cold pools are inversely correlated with the surrounding mid-level moisture. During the convectively suppressed phase, the recovery time is ~5-20 hours in relative weak wind condition with small air-sea fluxes. The recovery time is generally less than 6 hours during the active phase of MJO with moist mid-levels and stronger surface wind and air-sea fluxes.

  14. Biophysical Interactions within Step-Pool Mountain Streams Following Wildfire

    Science.gov (United States)

    Parker, A.; Chin, A.; O'Dowd, A. P.

    2014-12-01

    Recovery of riverine ecosystems following disturbance is driven by a variety of interacting processes. Wildfires pose increasing disturbances to riverine landscapes, with rising frequencies and magnitudes owing to warming climates and increased fuel loads. The effects of wildfire include loss of vegetation, elevated runoff and flash floods, erosion and deposition, and changing biological habitats and communities. Understanding process interactions in post-fire landscapes is increasingly urgent for successful management and restoration of affected ecosystems. In steep channels, steps and pools provide prominent habitats for organisms and structural integrity in high energy environments. Step-pools are typically stable, responding to extreme events with recurrence intervals often exceeding 50 years. Once wildfire occurs, however, intensification of post-fire flood events can potentially overpower the inherent stability of these systems, with significant consequences for aquatic life and human well-being downstream. This study examined the short-term response of step-pool streams following the 2012 Waldo Canyon Fire in Colorado. We explored interacting feedbacks among geomorphology, hydrology, and ecology in the post-fire environment. At selected sites with varying burn severity, we established baseline conditions immediately after the fire with channel surveys, biological assessment using benthic macroinvertebrates, sediment analysis including pebble counts, and precipitation gauging. Repeat measurements after major storm events over several years enabled analysis of the interacting feedbacks among post-fire processes. We found that channels able to retain the step-pool structure changed less and facilitated recovery more readily. Step habitats maintained higher percentages of sensitive macroinvertebrate taxa compared to pools through post-fire floods. Sites burned with high severity experienced greater reduction in the percentage of sensitive taxa. The decimation of

  15. Experimental investigation of particulate debris spreading in a pool

    Energy Technology Data Exchange (ETDEWEB)

    Konovalenko, A., E-mail: kono@kth.se [Division of Nuclear Power Safety, Royal Institute of Technology (KTH) , Roslagstullsbacken 21, Stockholm 106 91 (Sweden); Basso, S., E-mail: simoneb@kth.se [Division of Nuclear Power Safety, Royal Institute of Technology (KTH) , Roslagstullsbacken 21, Stockholm 106 91 (Sweden); Kudinov, P., E-mail: pkudinov@kth.se [Division of Nuclear Power Safety, Royal Institute of Technology (KTH) , Roslagstullsbacken 21, Stockholm 106 91 (Sweden); Yakush, S.E., E-mail: yakush@ipmnet.ru [Institute for Problems in Mechanics of the Russian Academy of Sciences, Ave. Vernadskogo 101 Bldg 1, Moscow 119526 (Russian Federation)

    2016-02-15

    Termination of severe accident progression by core debris cooling in a deep pool of water under reactor vessel is considered in several designs of light water reactors. However, success of this accident mitigation strategy is contingent upon the effectiveness of heat removal by natural circulation from the debris bed. It is assumed that a porous bed will be formed in the pool in the process of core melt fragmentation and quenching. Debris bed coolability depends on its properties and system conditions. The properties of the bed, including its geometry are the outcomes of the debris bed formation process. Spreading of the debris particles in the pool by two-phase turbulent flows induced by the heat generated in the bed can affect the shape of the bed and thus influence its coolability. The goal of this work is to provide experimental data on spreading of solid particles in the pool by large-scale two-phase flow. The aim is to provide data necessary for understanding of separate effects and for development and validation of models and codes. Validated codes can be then used for prediction of debris bed formation under prototypic severe accident conditions. In PDS-P (Particulate Debris Spreading in the Pool) experiments, air injection at the bottom of the test section is employed as a means to create large-scale flow in the pool in isothermal conditions. The test section is a rectangular tank with a 2D slice geometry, it has fixed width (72 mm), adjustable length (up to 1.5 m) and allows water filling to the depth of up to 1 m. Variable pool length and depth allows studying two-phase circulating flows of different characteristic sizes and patterns. The average void fraction in the pool is determined by video recording and subsequent image processing. Particles are supplied from the top of the facility above the water surface. Results of several series of PDS-P experiments are reported in this paper. The influence of the gas flow rate, pool dimensions, particle density

  16. Sampling and Pooling Methods for Capturing Herd Level Antibiotic Resistance in Swine Feces using qPCR and CFU Approaches

    DEFF Research Database (Denmark)

    Schmidt, Gunilla Veslemøy; Mellerup, Anders; Christiansen, Lasse Engbo

    2015-01-01

    The aim of this article was to define the sampling level and method combination that captures antibiotic resistance at pig herd level utilizing qPCR antibiotic resistance gene quantification and culture-based quantification of antibiotic resistant coliform indicator bacteria. Fourteen qPCR assays...... for commonly detected antibiotic resistance genes were developed, and used to quantify antibiotic resistance genes in total DNA from swine fecal samples that were obtained using different sampling and pooling methods. In parallel, the number of antibiotic resistant coliform indicator bacteria was determined...... in the same swine fecal samples. The results showed that the qPCR assays were capable of detecting differences in antibiotic resistance levels in individual animals that the coliform bacteria colony forming units (CFU) could not. Also, the qPCR assays more accurately quantified antibiotic resistance genes...

  17. Pool swell sub-scale testing and code comparison

    International Nuclear Information System (INIS)

    Elisson, K.

    1981-01-01

    The main objective of the experiment was to investigate the pool swell dynamics in general and the forces on the lowered central part of the diaphragm between drywell and wetwell in particular. Apart from the high speed camera pressure transducers and strain gauges were used to monitor the transient. Data was recorded on a 14 channel FM recorder and then digitalised and plotted. In total more than one hundred tests were performed including parametric variations of for example geometry, break flow, initial drywell pressure and initial water level. In parallel to this experiment pool swell calculations have been performed with the computer codes COPTA and STEALTH. COPTA which is a lumped mass code for pressure suppression containment analysis has a slug pool swell mode. STEALTH which is a general purpose lagrangian hydrodynamics code has been used in a 2-D axisymmetric version. The STEALTH code has been used to calculate the radial variations in the vertical displacement and velocity of the pool surface and to predict the load on the lowered central part of the diaphragm. A comparison between the calculations and the experimental data indicates that both codes are sufficiently correct in their description of the pool swell transient. (orig.)

  18. Immobilization of radioactive waste sludge from spent fuel storage pool

    International Nuclear Information System (INIS)

    Pavlovic, R.; Plecas, I.

    1998-01-01

    In the last forty years, in FR Yugoslavia, as result of the research reactors' operation and radionuclides application in medicine, industry and agriculture, radioactive waste materials of the different categories and various levels of specific activities were generated. As a temporary solution, these radioactive waste materials are stored in the two hanger type interim storages for solid waste and some type of liquid waste packed in plastic barrels, and one of three stainless steal underground containers for other types of liquid waste. Spent fuel elements from nuclear reactors in the Vinca Institute have been temporary stored in water filled storage pool. Due to the fact that the water in the spent fuel elements storage pool have not been purified for a long time, all metallic components submerged in the water have been hardly corroded and significant amount of the sludge has been settled on the bottom of the pool. As a first step in improving spent fuel elements storage conditions and slowing down corrosion in the storage spent fuel elements pool we have decided to remove the sludge from the bottom of the pool. Although not high, but slightly radioactive, this sludge had to be treated as radioactive waste material. Some aspects of immobilisation, conditioning and storage of this sludge are presented in this paper. (author

  19. Research progresses and future directions on pool boiling heat transfer

    Directory of Open Access Journals (Sweden)

    M. Kumar

    2015-12-01

    Full Text Available This paper reviews the previous work carried on pool boiling heat transfer during heating of various liquids and commodities categorized as refrigerants and dielectric fluids, pure liquids, nanofluids, hydrocarbons and additive mixtures, as well as natural and synthetic colloidal solutions. Nucleate pool boiling is an efficient and effective method of boiling because high heat fluxes are possible with moderate temperature differences. It is characterized by the growth of bubbles on a heated surface. It occurs during boiling of liquids for excess temperature ranging from 5 to 30 °C in various processes related to high vaporization of liquid for specific purposes like sugarcane juice heating for jaggery making, milk heating for khoa making, steam generation, cooling of electronic equipments, refrigeration and etcetera. In this review paper, pool boiling method during heating of liquids for specific purpose is depicted. It is inferred that enhancement in pool boiling heat transfer is a challenging and complex task. Also, recent research and use of various correlations for natural convection pool boiling is reviewed.

  20. SBWR PCCS vent phenomena and suppression pool mixing

    Energy Technology Data Exchange (ETDEWEB)

    Coddington, P. [Thermal-Hydraulics Lab., Paul Scherrer Institute, Villigen (Switzerland); Andreani, M. [Nuclear Engineering Lab., Swiss Federal Institute of Technology, Zurich (Switzerland)

    1995-09-01

    The most important phenomena influencing the effectiveness of the pressure suppression capability of the water pool within the Wetwell compartment of the SBWR Containment, during the period of Passive Containment Cooling System (PCCS) venting, have been critically reviewed. In addition, calculations have been carried-out to determine the condensation of the vented steam and the distribution of the energy deposited in the liquid pool. It has been found that a large contribution to the vapour suppression is due to condensation inside the vent pipe. The condensation rate of the steam inside the bubbles, produced at the vent exit, during their rise to the surface, may however be rather low, because of the large size bubbles. This can lead to vapour channelling to the Wetwell gas space. The above comments are likely to be ameliorated if the vent exit is a distributed source or sparger. Due to the large water flow rates within the {open_quotes}bubbly two-phase plume{close_quotes} generated by the gas injection, the water in the pool above the vent exit is likely to be heated nearly isothermally (perfect mixing). The effect of the suppression pool walls would be to enhance the recirculation and, consequently to promote mixing. The large size of the bubbles therein and of the walls on pool mixing are the most severe difficulties in extrapolating the results from scaled experiments to prototypical conditions.

  1. Water inventory management in condenser pool of boiling water reactor

    International Nuclear Information System (INIS)

    Gluntz, D.M.

    1996-01-01

    An improved system for managing the water inventory in the condenser pool of a boiling water reactor has means for raising the level of the upper surface of the condenser pool water without adding water to the isolation pool. A tank filled with water is installed in a chamber of the condenser pool. The water-filled tank contains one or more holes or openings at its lowermost periphery and is connected via piping and a passive-type valve (e.g., squib valve) to a high-pressure gas-charged pneumatic tank of appropriate volume. The valve is normally closed, but can be opened at an appropriate time following a loss-of-coolant accident. When the valve opens, high-pressure gas inside the pneumatic tank is released to flow passively through the piping to pressurize the interior of the water-filled tank. In so doing, the initial water contents of the tank are expelled through the openings, causing the water level in the condenser pool to rise. This increases the volume of water available to be boiled off by heat conducted from the passive containment cooling heat exchangers. 4 figs

  2. Determination of isotopomers in pools of molecules with polyisotopic elements

    International Nuclear Information System (INIS)

    Zyakun, A.M.; Brenninkmeijer, C.A.M.

    2002-01-01

    Polyisotopic element atoms that are present in a molecule form a pool of isotopomer molecules. Mono- and polyisotopomers are distinguished depending on the quantity of atoms of polyisotopic elements. Methodical approaches have been considered for the quantitative determination of the isotope composition of an element included in mono- and polyisotopic molecules. A possibility of the equally probable (homogeneous) and non-equally probable (non-homogeneous) distribution of isotopes of a polyisotopic element, the atoms of which have different positions in the molecule of polyisotopomer, has been shown. Factors disturbing the homogeneous distribution of isotopes of the element in the polyisotopomer pool have been revealed. When a polyisotopomer is involved in a mass-dependent process or reaction, the homogeneity of isotope distribution of the element is disturbed both in the residual and newly formed pools of polyisotopomer. By the example of CO 2 (polyisotopomer by oxygen) it has been shown that one can judge of the history of the analyzed pool formation by distribution of oxygen isotopes within this pool. The isotope content in the oxygen sites of polyisotopomer is a diagnostic feature of CO 2 involvement in the efflux from a reservoir or influx to the analyzed reservoir from an external source. (author)

  3. Health impact of disinfection by-products in swimming pools

    Directory of Open Access Journals (Sweden)

    Cristina M. Villanueva

    2012-12-01

    Full Text Available This article is focused on the epidemiological evidence on the health impacts related to disinfection by-products (DBPs in swimming pools, which is a chemical hazard generated as an undesired consequence to reduce the microbial pathogens. Specific DBPs are carcinogenic, fetotoxic and/or irritant to the airways according to experimental studies. Epidemiological evidence shows that swimming in pools during pregnancy is not associated with an increased risk of reproductive outcomes. An epidemiological study suggested an increased risk of bladder cancer with swimming pool attendance, although evidence is inconclusive. A higher prevalence of respiratory symptoms including asthma is found among swimming pool workers and elite swimmers, although the causality of this association is unclear. The body of evidence in children indicates that asthma is not increased by swimming pool attendance. Overall, the available knowledge suggests that the health benefits of swimming outweigh the potential health risks of chemical contamination. However, the positive effects of swimming should be enhanced by minimising potential risks.

  4. Evaluation of Total Coliform, Fecal Coliform and Residual Chlorine in Swimming Pools in Kermanshah on the Season, the type of Pool, Disinfection System and Source of Water Supply in the during of three years (2010-2012

    Directory of Open Access Journals (Sweden)

    K SHarafi

    2014-11-01

    From the results , although the pools of water quality parameters has been studied in almost ideal But in summer, especially on a female pools and pools with wells water supply source than other pools , to be more oversight .

  5. Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea

    KAUST Repository

    Mwirichia, Romano

    2016-01-13

    The candidate Division MSBL1 (Mediterranean Sea Brine Lakes 1) comprises a monophyletic group of uncultured archaea found in different hypersaline environments. Previous studies propose methanogenesis as the main metabolism. Here, we describe a metabolic reconstruction of MSBL1 based on 32 single-cell amplified genomes from Brine Pools of the Red Sea (Atlantis II, Discovery, Nereus, Erba and Kebrit). Phylogeny based on rRNA genes as well as conserved single copy genes delineates the group as a putative novel lineage of archaea. Our analysis shows that MSBL1 may ferment glucose via the Embden–Meyerhof–Parnas pathway. However, in the absence of organic carbon, carbon dioxide may be fixed via the ribulose bisphosphate carboxylase, Wood-Ljungdahl pathway or reductive TCA cycle. Therefore, based on the occurrence of genes for glycolysis, absence of the core genes found in genomes of all sequenced methanogens and the phylogenetic position, we hypothesize that the MSBL1 are not methanogens, but probably sugar-fermenting organisms capable of autotrophic growth. Such a mixotrophic lifestyle would confer survival advantage (or possibly provide a unique narrow niche) when glucose and other fermentable sugars are not available.

  6. Prediction of pool void fraction by new drift flux correlation

    International Nuclear Information System (INIS)

    Kataoka, I.; Ishii, M.

    1986-06-01

    A void fraction for a bubbling or boiling pool system is one of the important parameters in analyzing heat and mass transfer processes. Using the drift flux formulation, correlations for the pool void fraction have been developed in collaboration with a large number of experimental data. It has been found that the drift velocity in a pool system depends upon vessel diameter, system pressure, gas flux and fluid physical properties. The results show that the relative velocity and void fraction can be quite different from those predicted by conventional correlations. In terms of the rise velocity, four different regimes are identified. These are bubbly, churn-turbulent, slug and cap bubble regimes. The present correlations are shown to agree with the experimental data over wide ranges of parameters such as vessel diameter, system pressure, gas flux and physical properties. 39 refs., 41 figs

  7. Models for Pooled Time-Series Cross-Section Data

    Directory of Open Access Journals (Sweden)

    Lawrence E Raffalovich

    2015-07-01

    Full Text Available Several models are available for the analysis of pooled time-series cross-section (TSCS data, defined as “repeated observations on fixed units” (Beck and Katz 1995. In this paper, we run the following models: (1 a completely pooled model, (2 fixed effects models, and (3 multi-level/hierarchical linear models. To illustrate these models, we use a Generalized Least Squares (GLS estimator with cross-section weights and panel-corrected standard errors (with EViews 8 on the cross-national homicide trends data of forty countries from 1950 to 2005, which we source from published research (Messner et al. 2011. We describe and discuss the similarities and differences between the models, and what information each can contribute to help answer substantive research questions. We conclude with a discussion of how the models we present may help to mitigate validity threats inherent in pooled time-series cross-section data analysis.

  8. Human parvovirus PARV4 in plasma pools of Chinese origin.

    Science.gov (United States)

    Ma, Y-Y; Guo, Y; Zhao, X; Wang, Z; Lv, M-M; Yan, Q-P; Zhang, J-G

    2012-10-01

    Human parvovirus 4 (PARV4) is present in blood and blood products. As the presence and levels of PARV4 in Chinese source plasma pools have never been determined, we implemented real-time quantitative PCR to investigate the presence of PARV4 in source plasma pools in China. Results showed that 26·15% (51/195) of lots tested positive for PARV4. The amounts of DNA ranged from 2·83 × 10(3) copies/ml to 2·35×10(7) copies/ml plasma. The high level of PARV4 in plasma pools may pose a potential risk to recipients. Further studies on the pathogenesis of PARV4 are urgently required. © 2012 The Author(s). Vox Sanguinis © 2012 International Society of Blood Transfusion.

  9. Lin28a regulates germ cell pool size and fertility

    Science.gov (United States)

    Shinoda, Gen; de Soysa, T. Yvanka; Seligson, Marc T.; Yabuuchi, Akiko; Fujiwara, Yuko; Huang, Pei Yi; Hagan, John P.; Gregory, Richard I.; Moss, Eric G.; Daley, George Q.

    2013-01-01

    Overexpression of LIN28A is associated with human germ cell tumors and promotes primordial germ cell (PGC) development from embryonic stem cells in vitro and in chimeric mice. Knockdown of Lin28a inhibits PGC development in vitro, but how constitutional Lin28a deficiency affects the mammalian reproductive system in vivo remains unknown. Here, we generated Lin28a knockout (KO) mice and found that Lin28a deficiency compromises the size of the germ cell pool in both males and females by affecting PGC proliferation during embryogenesis. Interestingly however, in Lin28a KO males the germ cell pool partially recovers during postnatal expansion, while fertility remains impaired in both males and females mated to wild type mice. Embryonic overexpression of let-7, a microRNA negatively regulated by Lin28a, reduces the germ cell pool, corroborating the role of the Lin28a/let-7 axis in regulating the germ lineage. PMID:23378032

  10. Safety analysis of a pool Genesis II irradiator

    International Nuclear Information System (INIS)

    Rodrigues Junior, Ary de A.

    2011-01-01

    The Genesis II Irradiator manufactured by GRAT * STAR Inc. (USA) is a category III gamma irradiator in which the sealed source is contained in a water filled storage pool and is shielded permanently, i.e. the material has to move down to the source. Even though the pool is 5.6 m deep, what would happen if the water level lowered? There are a series of safety devices that will avoid this situation and calculations show that the water level has to be very low in order to deliver a significant dose; moreover, only in case a person remains at the border of the pool for a long time this would be risky. In conclusion, it is practically impossible for someone to be exposed to radiation from a Genesis II Irradiator source. (author)

  11. Core disruptive accident and recriticality analysis with FX2-POOL

    International Nuclear Information System (INIS)

    Abramson, P.B.

    1976-01-01

    The current state of development of FX2-POOL, a two-dimensional hydrodynamic, thermodynamic and neutronic scoping model for Hypothetical Core Disruptive Accident analysis is described. Checkout comparisons to VENUS for prompt burst conditions were good. Use of FX2-POOL to examine the importance of fuel to steel heat transfer during a prompt burst indicates that heat transfer plays no important role on that time scale. Scoping studies of material thermohydrodynamics for about 20 to 30 milliseconds following the prompt burst indicate that heat transfer is important on the time scale necessary for the CDA bubble to grow to the size of the original core. Preliminary results are presented for energetics of boiling fuel steel pools which are forced recritical by local surface pressurization

  12. CFD modeling of pool swell during large break LOCA

    International Nuclear Information System (INIS)

    Yan, Jin; Bolger, Francis; Li, Guangjun; Mintz, Saul; Pappone, Daniel

    2009-01-01

    GE had conducted a series of one-third scale three-vent air tests in support the horizontal vent pressure suppression system used in Mark III containment design for General Electric BWR plants. During the test, the air-water interface has been tracked by conductivity probes. There are many pressure monitors inside the test rig. The purpose of the test was to provide a basis for the pool swell load definition for the Mark III containment. In this paper, a transient 3-Dimensional CFD model of the one-third scale Mark III suppression pool swell process is constructed. The Volume of Fluid (VOF) multiphase model is used to explicitly track the interface between the water liquid and the air. The CFD results such as flow velocity, pressure, interface locations are compared to those from the test. Through the comparisons, a technical approach to numerically model the pool swell phenomenon is established and benchmarked. (author)

  13. Analysis of radiation shields of BNPP spent fuel pool

    International Nuclear Information System (INIS)

    Ayoobian, N.; Hadad, K.; Nematollahi, M. R.

    2007-01-01

    Radioactive protection is one of the most important subjects in nuclear power plants safety. Analysis of BNPP spent fuel pool shielding , as a main source of energetic γ-rays was the main goal of this project. Firstly, we simulated the reactor core using WIMSD-4 neutronic code and the amount of fission product in the fuel assembly (FA) was calculated during the reactor operation. Then, by obtaining the results from the previous calculation and by using MCNP4C nuclear code , the intensity of γ-rays was obtained in layers of spent fuel pool shields. The results have shown that no significant γ-rays passed through these shields. Finally, an accident and resulting exposure dose above the pool was analyzed

  14. Insertion sequences enrichment in extreme Red sea brine pool vent

    KAUST Repository

    Elbehery, Ali H. A.

    2016-12-03

    Mobile genetic elements are major agents of genome diversification and evolution. Limited studies addressed their characteristics, including abundance, and role in extreme habitats. One of the rare natural habitats exposed to multiple-extreme conditions, including high temperature, salinity and concentration of heavy metals, are the Red Sea brine pools. We assessed the abundance and distribution of different mobile genetic elements in four Red Sea brine pools including the world’s largest known multiple-extreme deep-sea environment, the Red Sea Atlantis II Deep. We report a gradient in the abundance of mobile genetic elements, dramatically increasing in the harshest environment of the pool. Additionally, we identified a strong association between the abundance of insertion sequences and extreme conditions, being highest in the harshest and deepest layer of the Red Sea Atlantis II Deep. Our comparative analyses of mobile genetic elements in secluded, extreme and relatively non-extreme environments, suggest that insertion sequences predominantly contribute to polyextremophiles genome plasticity.

  15. Spent nuclear fuel storage pool thermal-hydraulic analysis

    International Nuclear Information System (INIS)

    Gay, R.R.

    1984-01-01

    Storage methods and requirements for spent nuclear fuel at U.S. commercial light water reactors are reviewed in Section 1. Methods of increasing current at-reactor storage capabilities are also outlined. In Section 2 the development of analytical methods for the thermal-hydraulic analysis of spent fuel pools is chronicled, leading up to a discussion of the GFLOW code which is described in Section 3. In Section 4 the verification of GFLOW by comparisons of the code's predictions to experimental data taken inside the fuel storage pool at the Maine Yankee nuclear power plant is presented. The predictions of GFLOW using 72, 224, and 1584 node models of the storage pool are compared to each other and to the experimental data. An example of thermal licensing analysis for Maine Yankee using the GFLOW code is given in Section 5. The GFLOW licensing analysis is compared to previous licensing analysis performed by Yankee Atomic using the RELAP-4 computer code

  16. High efficiency pool filtering systems utilising variable frequency drives

    Energy Technology Data Exchange (ETDEWEB)

    Hameiri, Z.; Sproul, A.B. [School of Photovoltaic and Renewable Energy Engineering, UNSW, Sydney, NSW 2052 (Australia); Spooner, T. [School of Electrical Engineering and Telecommunications, UNSW, Sydney, NSW 2052 (Australia)

    2009-02-15

    Over 1 year, private swimming pools in Australia will typically consume 1680 GWh of electricity, producing 2130 kt of CO{sub 2}. Redesigning a pool's filtration system and using it more efficiently can reduce the energy use, and hence the CO{sub 2} production, by a significant amount. This paper describes experimental measurements carried out on a new design of pool pump system. Initial experiments using a variable frequency drive (VFD) with a standard, single phase pump/motor system have achieved energy savings of 40%. Utilising a VFD and a three phase pump/motor energy savings of 61% have been achieved, without degrading the system performance. (author)

  17. Analyzing data distribution on disk pools for dCache

    Energy Technology Data Exchange (ETDEWEB)

    Halstenberg, S; Jung, C; Ressmann, D [Forschungszentrum Karlsruhe, Steinbuch Centre for Computing, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany)

    2010-04-01

    Most Tier-1 centers of LHC Computing Grid are using dCache as their storage system. dCache uses a cost model incorporating CPU and space costs for the distribution of data on its disk pools. Storage resources at Tier-1 centers are usually upgraded once or twice a year according to given milestones. One of the effects of this procedure is the accumulation of heterogeneous hardware resources. For a dCache system, a heterogeneous set of disk pools complicates the process of weighting CPU and space costs for an efficient distribution of data. In order to evaluate the data distribution on the disk pools, the distribution is simulated in Java. The results are discussed and suggestions for improving the weight scheme are given.

  18. Inferring local ecological processes amid species pool influences

    DEFF Research Database (Denmark)

    Lessard, Jean-Philippe; Belmaker, Jonathan; Myers, Jonathan A.

    2012-01-01

    studies, null models of community structure, and ecologically explicit definitions of the species pool as a means to compare predominant ecological processes among regions. By uniting concepts and tools from community ecology and macroecology, this approach might facilitate synthesis and resolve many......Resolving contingencies in community ecology requires comparative studies of local communities along broad-scale environmental gradients and in different biogeographic regions. However, comparisons of local ecological processes among regions require a synthetic understanding of how the species pool...... of potential community members influences the structure of ecological communities. Here, we outline an integrative approach for quantifying local ecological processes while explicitly accounting for species pool influences. Specifically, we highlight the utility of combining geographically replicated local...

  19. CAREM 25: Suppression pool cooling and purification system

    International Nuclear Information System (INIS)

    Carlevaris, Rodolfo; Patrignani, Alberto; Vindrola, Carlos; Palmerio, Hector D.; Quiroz, Horacio; Ramilo, Lucia B.

    2000-01-01

    The suppression pool cooling and purification system has the following main functions: purify and cool water from the suppression pool, cool and send water to the residual heat extraction system, and transfer water to the fuel element transference channel. In case of Loss of Coolant Accident (LOCA), the system sends water from the suppression pool to the spray network, thus cooling and reducing pressure in the primary containment. The system has been designed in accordance with the requirements of the following standards: ANSI/ANS 52.1; ANSI/ANS 57.2; ANSI/ANS 56.2; ANSI/ANS 59.1; ANSI/ANS 58.3; ANSI/ANS 58.9; and ANSI/ANS 56.5. The design of the system fulfils all the assigned functions. (author)

  20. CAREM-25. Suppression Pool Cooling and Purification System

    International Nuclear Information System (INIS)

    Carlevaris, Rodolfo; Palmerio, D.; Patrignani, A.; Quiroz, H.; Ramilo, L.; Vindrola, C.

    2000-01-01

    The Suppression Pool Cooling and Purification System has the following main functions: purify and cool water from the Suppression Pool, cool and send water to the Residual Heat Extraction System, and transfer water to the Fuel Element Transference Channel. In case of Loss of Coolant Accident (LOCA), the system sends water from the Suppression Pool to the spray network, thus cooling and reducing pressure in the primary containment.The system has been designed in accordance with the requirements of the following standards ANSI/ANS 52.1 [1], ANSI/ANS 57.2 [2], ANSI/ANS 56.2 [3], ANSI/ANS 59.1 [4] ANSI/ANS 58.3 [5], ANSI/ANS 58.9 [6], and ANSI/ANS 56.5 [7]. The design of the system fulfils all the assigned functions

  1. Analysis of the loss of pool cooling accident in a PWR spent fuel pool with MAAP5

    International Nuclear Information System (INIS)

    Wu, Xiaoli; Li, Wei; Zhang, Yapei; Tian, Wenxi; Su, Guanghui; Qiu, Suizheng

    2014-01-01

    Highlights: • A PWR spent fuel pool was modeled by using MAAP5. • Loss of pool cooling severe accident scenarios were studied. • Loss of pool cooling accidents with two mitigation measures were analyzed. - Abstract: The Fukushima Daiichi nuclear accident shows that it is necessary to study potential severe accidents and corresponding mitigation measures for the spent fuel pool (SFP) of a nuclear power plant (NPP). This paper presents the analysis of loss of pool cooling accident scenarios and the discussion of mitigation measures for the SFP at a pressurized water reactor (PWR) NPP with the MAAP5 code. Analysis of uncompensated loss of water due to the loss of pool cooling with different initial pool water levels of 12.2 m (designated as a reference case) and 10.7 m have been performed based on a MAAP5 input model. Scenarios of the accident such as overheating of uncovered fuel assemblies, oxidation of claddings and hydrogen generation, loss of intactness of fuel rod claddings, and release of radioactive fission products were predicted with the assumption that mitigation measures were unavailable. The results covered a broad spectrum of severe accident evaluations in the SFP. Furthermore, as important mitigation measures, the effects of recovering the SFP cooling system and makeup water in SFP on the accident progressions have also been investigated respectively based on the events of pool water boiling and spent fuels uncovery. Based upon the reference case, three cases with the recovery of SFP cooling system and three other cases with makeup water in SFP have been studied. The results showed that, severe accident might happen if SFP cooling system was not restored timely before the spent fuels started to become uncovered; spent fuels could be completely submerged and severe accident might be avoided if SFP makeup water system provided water with a mass flow rate larger than the average evaporation rate defined as the division of pool water mass above the

  2. Temperature dependent fission product removal efficiency due to pool scrubbing

    Energy Technology Data Exchange (ETDEWEB)

    Uchida, Shunsuke, E-mail: suchida@iae.or.jp [Institute of Applied Energy, 1-14-2, Nishi-Shimbashi, Minato-ku, Tokyo 105-0003 (Japan); Itoh, Ayumi; Naitoh, Masanori; Okada, Hidetoshi; Suzuki, Hiroyuki [Institute of Applied Energy, 1-14-2, Nishi-Shimbashi, Minato-ku, Tokyo 105-0003 (Japan); Hanamoto, Yukio [KAKEN, Inc., 1044, Hori-machi, Mito 310-0903 (Japan); Osakabe, Masahiro [Tokyo University of Marine Science & Technology, Koutou-ku, Tokyo 135-8533 (Japan); Fujikawa, Masahiro [Japan Broadcasting Corporation, 2-2-1, Jinnan, Shibuya-ku, Tokyo 150-8001 (Japan)

    2016-03-15

    Highlights: • Pool temperature effects on the FP removal were not clearly concluded in the previous publications. • It was confirmed that the removal efficiency decreased with temperature around the boiling point. • A modified empirical formula for FP removal was proposed as a function of sub-cooling temperature. • DF could be predicted with an accuracy within a factor of 2 with the proposed formula. - Abstract: The wet-well of boiling water reactors plays important roles not only to suppress the pressure in the primary containment vessel due to steam scrubbing effects during severe accidents but also to mitigate release of radioactive fission products (FP), aerosols and particulates, into the environment. The effects of steam scrubbing in the wet-well on FP removal have been well studied and reported by changing major parameters determining the removal efficiencies, e.g., aerosol diameters, submergence (depth of scrubbing nozzles) and steam/non-condensable gas volume fraction. Unfortunately, the effects of pool temperature on the FP removal were not clearly concluded in the previous publications, though it would be easily expected that boiling in the pool resulted in reduced aerosol removal efficiency. In order to determine the temperature effects on FP removal efficiency, amounts of cesium in aerosols released from scrubbing pool were measured by changing pool temperature in mini and medium scale scrubbing experiments, and then, it was confirmed that the removal efficiency clearly decreased with temperature around the boiling point. Then, a modified empirical formula to express the FP removal around the boiling point temperature was proposed as a function of sub-cooling temperature by applying the effective steam volume fraction, which was designated as the volume ratio of condensed steam in the pool versus the sum of input steam and non-condensable gas. By comparing the measured removal efficiency with the calculated, it was validated that the

  3. The pool chlorine hypothesis and asthma among boys.

    LENUS (Irish Health Repository)

    Cotter, A

    2012-01-31

    Swimming pool sanitation has largely been concerned with the microbiological quality of pool water, which is normally treated using a number of chlorine products. Recent studies have pointed to the potential hazards of chlorine by-products to the respiratory epithelium, particularly in indoor, poorly ventilated, pools. The aim of our study was to elucidate whether chronic exposure to indoor chlorinated swimming pools was associated with an increased likelihood of the development of asthma in boys. METHODS: The subjects were boys aged between 6 and 12 years. Data was collected by means of parental responses to a standardized asthma questionnaire (ISAAC: International Study of Asthma and Allergies in Childhood), supplemented with additional questions regarding frequency of attendance, number of years attendance, whether the child is a swimming team member. The questionnaire return rate was 71\\/% (n = 121). 23 boys were excluded on the basis that they had asthma before they started swimming (n = 97). There was a significant association between number of years a boy had been swimming and the likelihood of wheezing in the last 12 months (p = 0.009; OR = 1.351; 95% CI = 1.077-1.693) and diagnosed asthma (p = 0.046; OR = 1.299; 95% CI = 1.004-1.506). The greater the number the number of years a boy had been attending an indoor, chlorinated pool, the greater the likelihood of wheezing in the last 12 months or "had asthma". Age, parental smoking habits and being a swimming team member had no association with any of the asthma variables examined. Swimming pool attendance may be a risk factor in asthma in boys.

  4. Temperature dependent fission product removal efficiency due to pool scrubbing

    International Nuclear Information System (INIS)

    Uchida, Shunsuke; Itoh, Ayumi; Naitoh, Masanori; Okada, Hidetoshi; Suzuki, Hiroyuki; Hanamoto, Yukio; Osakabe, Masahiro; Fujikawa, Masahiro

    2016-01-01

    Highlights: • Pool temperature effects on the FP removal were not clearly concluded in the previous publications. • It was confirmed that the removal efficiency decreased with temperature around the boiling point. • A modified empirical formula for FP removal was proposed as a function of sub-cooling temperature. • DF could be predicted with an accuracy within a factor of 2 with the proposed formula. - Abstract: The wet-well of boiling water reactors plays important roles not only to suppress the pressure in the primary containment vessel due to steam scrubbing effects during severe accidents but also to mitigate release of radioactive fission products (FP), aerosols and particulates, into the environment. The effects of steam scrubbing in the wet-well on FP removal have been well studied and reported by changing major parameters determining the removal efficiencies, e.g., aerosol diameters, submergence (depth of scrubbing nozzles) and steam/non-condensable gas volume fraction. Unfortunately, the effects of pool temperature on the FP removal were not clearly concluded in the previous publications, though it would be easily expected that boiling in the pool resulted in reduced aerosol removal efficiency. In order to determine the temperature effects on FP removal efficiency, amounts of cesium in aerosols released from scrubbing pool were measured by changing pool temperature in mini and medium scale scrubbing experiments, and then, it was confirmed that the removal efficiency clearly decreased with temperature around the boiling point. Then, a modified empirical formula to express the FP removal around the boiling point temperature was proposed as a function of sub-cooling temperature by applying the effective steam volume fraction, which was designated as the volume ratio of condensed steam in the pool versus the sum of input steam and non-condensable gas. By comparing the measured removal efficiency with the calculated, it was validated that the

  5. Gene Therapy

    Science.gov (United States)

    Gene therapy Overview Gene therapy involves altering the genes inside your body's cells in an effort to treat or stop disease. Genes contain your ... that don't work properly can cause disease. Gene therapy replaces a faulty gene or adds a new ...

  6. Study of Microbial Contamination of the Public Swimming Pools with Escherichia coli and Pseudomonas aeruginosa and Their Physical Parameters in Kermanshah, Iran

    Directory of Open Access Journals (Sweden)

    Afsaneh Haghmorad Korasti

    2016-09-01

    Full Text Available Background and Objectives: Public swimming pools' waters are contaminated with a wide variety of pathogenic microorganisms and are a suitable environment for transmission of different diseases. The aim of this study was to investigate the microbial contamination of the public swimming pools' waters with Escherichia coli and Pseudomonas aeruginosa and to determine certain parameters such as residual chlorine, pH, temperature and turbidity in these pools' waters in Kermanshah. In this descriptive, cross-sectional study, 129 water samples were taken from all active pools in Kermanshah and their bacteriologic and physicochemical properties were investigated. Phosphatase alkaline (PHO-A gene was used for molecular confirmation of E. coli isolates, and exotoxin A (ETA gene in PCR was employed to confirm pathogenicity of P. aeruginosa isolates. Data were analyzed by chi-square and t-test. p0.05. Conclusion: The results of this study indicated that appropriate amount of residual chlorine caused reduction in microbial contamination in the public swimming pools' waters in Kermanshah.

  7. Sodium-water reaction in double pool LMFBR, (5)

    International Nuclear Information System (INIS)

    Yoshida, Kazuo; Kumagai, Hiromichi; Nishi, Yoshihisa; Uotani, Masaki

    1990-01-01

    Experiments were conducted using a 1/5 scale model of the Double Pool in order to evaluate a pressure rise caused by a large scale sodium-water reaction. The experiments were focused on the pressure rise caused by the piston motion of liquid sodium. It appeared from the results that the magnitude of this pressure rise depends on the depth of reaction point, and that a pressure rise more than 1 MPa would arise in the real Double Pool plant. A new design of steam generator is proposed to mitigate the pressure rise. (author)

  8. The CERN Electronics Pool moves to Building 13

    CERN Multimedia

    PH Department

    2010-01-01

    After 32 years in Building 4 R‐050, the CERN Electronics Pool will re‐open in Building 13 R009 on Monday 13 December at 10:00 As of this date, you will be able to come and choose the instruments you want: oscilloscopes and other measurement instruments, low and high voltage power supplies, modular instrumentation, etc. Please do not hesitate to consult the catalogue and give us any input you may have. The CERN Electronics Pool operates on a self-service basis (with a CERN budget code) and is available for any help you may need.

  9. Carbon dynamics in peatland pool systems: the role of light

    Science.gov (United States)

    Pickard, Amy; Heal, Kate; McLeod, Andy; Dinsmore, Kerry

    2016-04-01

    Open-water pools are widespread in peatlands and are considered to represent biogeochemical hotspots within the peatland landscape. However the contribution of pool systems to wider peatland C cycling has not been quantified fully and there is a lack of knowledge of the role of photochemical processes in such environments. In this study, light exposure experiments were conducted in two contrasting pools to test the reactivity of aquatic C. The first study site was located at Cross Lochs (CL), Forsinard, in the Flow Country of Northern Scotland, in a 412 m2 pool characterised by low dissolved organic carbon (DOC) concentrations (˜15 mg C L-1). The second site was located at Red Moss of Balerno (RM), a raised bog in central Scotland, in a 48 m2 pool with high DOC concentrations (˜35 mg C L-1). Experiments took place over 9 days in situ at each pool in mid-summer 2015, with 500 mL water samples contained in bags transparent to sunlight and in opaque control bags. After field exposure, optical, chemical and stable C isotope analyses were conducted on the samples. Significant differences in biogeochemical cycling of DOC were detected between the two systems, with DOC losses as a percentage of the total C pool 15% higher at RM than at CL after light exposure. The mean DOC concentration of light exposed samples at RM declined steeply initially, with 83% observed DOC degradation occurring by day 3 of the experiment. Total losses of 7.9 mg DOC L-1were observed in light exposed samples at RM, along with decreasing E4:E6 ratios, suggesting that material remaining at the end of the experiment was humified. Depletion of DOC was positively correlated with production of CO2 at both sites, with concentrations of up to 4.3 mg CO2-C L-1 recorded at RM. Stable C isotope signatures at both sites were altered under light treatment, as demonstrated by the production of enriched δ13C-DOC (+0.46 ‰ relative to opaque bags) and depleted δ13C-DIC (-0.97 ‰ relative to opaque bags) at

  10. Software for the Design of Swimming Pool Dehumidifiers Units

    Science.gov (United States)

    Rubina, Aleš; Blasinski, Petr; Tesař, Zdeněk

    2013-06-01

    The article deals with the description and solution of physical phenomena taking place during evaporation of water. The topicality of the theme is given a number of built indoor swimming pool and wellness centers at present. In addressing HVAC systems serving these areas, it is necessary to know the various design parameters in the interior including the water temperature as the pool temperature and humidity. Following is a description of the calculation module, air handling units, including optimizing the settings of the physical changes in order to ensure the lowest energy consumption for air treatment and required maintaining internal microclimate parameters.

  11. Cryptosporidium and Giardia in Swimming Pools, Atlanta, Georgia

    Centers for Disease Control (CDC) Podcasts

    2008-05-29

    In this podcast, Dan Rutz speaks with Dr. Joan Shields, a guest researcher with the Healthy Swimming Program at CDC, about an article in June 2008 issue of Emerging Infectious Diseases reporting on the results of a test of swimming pools in the greater Atlanta, Georgia area. Dr. Shields tested 160 pools in metro Atlanta last year for Cryptosporidium and Giardia. These germs cause most recreational water associated outbreaks.  Created: 5/29/2008 by Emerging Infectious Diseases.   Date Released: 5/29/2008.

  12. Measure Guideline: Replacing Single-Speed Pool Pumps with Variable Speed Pumps for Energy Savings

    Energy Technology Data Exchange (ETDEWEB)

    Hunt, A.; Easley, S.

    2012-05-01

    The report evaluates potential energy savings by replacing traditional single-speed pool pumps with variable speed pool pumps, and provide a basic cost comparison between continued uses of traditional pumps verses new pumps. A simple step-by-step process for inspecting the pool area and installing a new pool pump follows.

  13. Measure Guideline. Replacing Single-Speed Pool Pumps with Variable Speed Pumps for Energy Savings

    Energy Technology Data Exchange (ETDEWEB)

    Hunt, A. [Building Media and the Building America Retrofit Alliance (BARA), Wilmington, DE (United States); Easley, S. [Building Media and the Building America Retrofit Alliance (BARA), Wilmington, DE (United States)

    2012-05-01

    This measure guideline evaluates potential energy savings by replacing traditional single-speed pool pumps with variable speed pool pumps, and provides a basic cost comparison between continued uses of traditional pumps verses new pumps. A simple step-by-step process for inspecting the pool area and installing a new pool pump follows.

  14. Characteristics of pools used by adult summer steelhead oversummering in the New River, California

    Science.gov (United States)

    Rodney J. Nakamoto

    1994-01-01

    Abstract - I assessed characteristics of pools used by oversummering adults of summer steelhead Oncorhynchus mykiss between July and October 1991 in the New River, northwestern California. Most fish occupied channel confluence pools and other pools of moderate size (200-1,200 m 2); these pools had less than 35% substrate embeddedness and mean water depths of about 1.0...

  15. Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2-mutated fibroblasts.

    Science.gov (United States)

    Frangini, Miriam; Rampazzo, Chiara; Franzolin, Elisa; Lara, Mari-Carmen; Vilà, Maya R; Martí, Ramon; Bianchi, Vera

    2009-02-01

    Mitochondrial thymidine kinase (TK2) catalyzes the phosphorylation of thymidine in mitochondria. Its function becomes essential for dTTP synthesis in noncycling cells, where cytosolic dTTP synthesis via R1/R2 ribonucleotide reductase and thymidine kinase 1 is turned down. Mutations in the nuclear gene for TK2 cause a fatal mtDNA depletion syndrome. Only selected cell types are affected, suggesting that the other cells compensate for the TK2 deficiency by adapting the enzyme network that regulates dTTP synthesis outside S-phase. Here we looked for such metabolic adaptation in quiescent cultures of fibroblasts from two TK2-deficient patients with a slow-progressing syndrome. In cell extracts, we measured the activities of TK2, deoxycytidine kinase, thymidine phosphorylase, deoxynucleotidases and the amounts of the three ribonucleotide reductase subunits. Patient cells contained 40% or 5% TK2 activity and unchanged activities of the other enzymes. However, their mitochondrial and cytosolic dTTP pools were unchanged, and also the overall composition of the dNTP pools was normal. TK2-dependent phosphorylation of [(3)H]thymidine in intact cells and the turnover of the dTTP pool showed that even the fibroblasts with 5% residual TK2 activity synthesized dTTP at an almost normal rate. Normal fibroblasts apparently contain more TK2 than needed to maintain dTTP during quiescence, which would explain why TK2-mutated fibroblasts do not manifest mtDNA depletion despite their reduced TK2 activity.

  16. Swimming pools and intra-city climates: Influences on residential ...

    African Journals Online (AJOL)

    While determinants such as household income, regional climate, water price, property size and household occupancy have been comprehensively studied and modelled, other determinants such as swimming pools and intra-city climates have not. This study examines residential water consumption in the City of Cape Town ...

  17. 75 FR 76975 - 2015 Resource Pool-Sierra Nevada Region

    Science.gov (United States)

    2010-12-10

    ... and allocation criteria set forth in the Marketing Plan. Comment: Four commentors requested that, in... Western Area Power Administration (Western), a Federal power marketing administration of DOE, announces the Final 2015 Resource Pool allocations pursuant to its 2004 Power Marketing Plan (Marketing Plan...

  18. THE PREDICTION OF VOID VOLUME IN SUBCOOLED NUCLEATE POOL BOILING

    Energy Technology Data Exchange (ETDEWEB)

    Duke, E. E. [General Dynamics, San Diego, CA (United States)

    1963-11-15

    A three- step equation was developed that adequately describes the average volume of vapor occurring on a horizontal surface due to nucleate pool boiling of subcooled water. Since extensive bubble frequency data are lacking, the data of others were combined with experimental observations to make predictions of void volume at ambient pressure with various degrees of subcooling. (auth)

  19. Stabilization of reactor fuel storage pool-TTP

    International Nuclear Information System (INIS)

    Sevigny, G.

    1994-10-01

    The proposed work includes evaluating standard and improved technologies an designing an integrated demonstration system to clean the water and sludge the fuel storage pools. The water released would meet drinking water standards and tritium standards. The volume of radioactive sludge would be reduced by partial separation of the sludge and radionuclides and eventual solidification of the hazardous and radioactive waste. The scope of the wo includes a survey of needs and applicable technologies, system engineering evaluation, conceptual design, detailed design, fabrication of the integrat demonstration system, and testing of the system. The survey task will locate potential specific customers within the DOE complex, and outside of the DOE complex throughout the United States, that be able to utilize the narrowly focused technology to stabilize/shutdown reactor fuel storage pools, responsible parties will be located and asked respond to a survey about their specific process requirements. Literature searches will be run through technical and scientific databases to locate technologies that may be an improvement over the standard baselined technol for cleanup of radioactively-contaminated pools. Systems engineering will provide decision analysis support for the development, evaluation, design, test functions of the treatment of pool water and sludge

  20. Spikes and memory in (Nord Pool) electricity price spot prices

    DEFF Research Database (Denmark)

    Proietti, Tomasso; Haldrup, Niels; Knapik, Oskar

    Electricity spot prices are subject to transitory sharp movements commonly referred to as spikes. The paper aims at assessing their effects on model based inferences and predictions, with reference to the Nord Pool power exchange. We identify a spike as a price value which deviates substantially...

  1. Pump/heat exchanger assembly for pool-type reactor

    International Nuclear Information System (INIS)

    Nathenson, R.D.; Slepian, R.M.

    1987-01-01

    A heat exchanger and pump assembly comprising a heat exchanger including a housing for defining an annularly shaped cavity and supporting therein a plurality of heat transfer tubes. A pump is disposed beneath the heat exchanger and is comprised of a plurality of flow couplers disposed in a circular array. Each flow coupler is comprised of a pump duct for receiving a first electrically conductive fluid, i.e. the primary liquid metal, from a pool thereof, and a generator duct for receiving a second electrically conductive fluid, i.e. the intermediate liquid metal. The primary liquid metal is introduced from the reactor pool into the top, inlet ends of the tubes, flowing downward therethrough to be discharged from the tubes' bottom ends directly into the reactor pool. The primary liquid metal is variously introduced into the pump ducts directly from the reactor pool, either from the bottom or top end of the flow coupler. The intermediate fluid introduced into the generator ducts via the inlet duct and inlet plenum and after leaving the generator ducts passes through the annular cavity of the exchanger to cool the primary liquid in the tubes. The annular magnetic field of the pump is produced by a circular array of electromagnets having hollow windings cooled by a flow of the intermediate metal. (author)

  2. Classical pooling of cross-section and time series data

    International Nuclear Information System (INIS)

    Nuamah, N.N.N.N.

    2000-04-01

    This paper discusses the classical pooling of cross-section and time series data. The re-expressions of the normal equations of this model are given to indicate the source of the paradox that arises in the estimation of the regression coefficient. (author)

  3. Reactor TRIGA PUSPATI (RTP) spent fuel pool conceptual design

    International Nuclear Information System (INIS)

    Mohd Fazli Zakaria; Tonny Lanyau; Ahmad Nabil Ab Rahim

    2010-01-01

    Reactor TRIGA PUSPATI (RTP) is the one and only research reactor in Malaysia that has been safely operated and maintained since 1982. In order to enhance technical capabilities and competencies especially in nuclear reactor engineering a feasibility study on RTP power upgrading was proposed to serve future needs for advance nuclear science and technology in the country with the capability of designing and develop reactor system. The need of a Spent Fuel Pool begins with the discharge of spent fuel elements from RTP for temporary storage that includes all activities related to the storage of fuel until it is either sent for reprocessed or sent for final disposal. To support RTP power upgrading there will be major RTP systems replacement such as reactor components and a new temporary storage pool for fuel elements. The spent fuel pool is needed for temporarily store the irradiated fuel elements to accommodate a new reactor core structure. Spent fuel management has always been one of the most important stages in the nuclear fuel cycle and considered among the most common problems to all countries with nuclear reactors. The output of this paper will provide sufficient information to show the Spent Fuel Pool can be design and build with the adequate and reasonable safety assurance to support newly upgraded TRIGA PUSPATI TRIGA Research Reactor. (author)

  4. Designing ARVs Patent Pool Up to Trade & Policy Evolutionary Dynamics.

    Science.gov (United States)

    Dionisio, Daniele; Racalbuto, Vincenzo; Messeri, Daniela

    2010-01-19

    Patent pools for second and third-line Fixed Dose Combination (FDC) antiretroviral drugs (ARVs) should not be delayed as they are instrumental to urgent public health needs in the under-served markets.Nonetheless, multinational originator companies still seem to perceive patent pooling for ARVs as a minefield that would offer the generic competitors lots of deeply exploitable opportunities, to the detriment of patent owner's rights.This paper analyses the brand industry concerns, while looking for a strategy up to a really equitable and free world market, without any discrimination between end-users in wealthy and resource-limited countries.This strategy would urge partnerships between originator companies first to make newer FDC ARVs quickly available and allow patent pool agreements with generic counterparts to be negotiated straight afterwards.The patent pool strategy highlighted in this paper would assert the primacy of health over for-profit policies, while aligning with the 61(st) WHO's Assembly recommendations and G7, G8 and World Trade Organisation's warnings and pledges against trade protectionism.

  5. Mitigated subsurface transfer line leak resulting in a surface pool

    Energy Technology Data Exchange (ETDEWEB)

    SCOTT, D.L.

    1999-02-08

    This analysis evaluates the mitigated consequences of a potential waste transfer spill from an underground pipeline. The spill forms a surface pool. One waste composite, a 67% liquid, 33% solid, from a single shell tank is evaluated. Even drain back from a very long pipeline (50,000 ft), does not pose dose consequences to the onsite or offsite individual above guideline values.

  6. Mitigated subsurface transfer line leak resulting in a surface pool

    International Nuclear Information System (INIS)

    SCOTT, D.L.

    1999-01-01

    This analysis evaluates the mitigated consequences of a potential waste transfer spill from an underground pipeline. The spill forms a surface pool. One waste composite, a 67% liquid, 33% solid, from a single shell tank is evaluated. Even drain back from a very long pipeline (50,000 ft), does not pose dose consequences to the onsite or offsite individual above guideline values

  7. Gamification is broken. An interview with Steven Poole

    Directory of Open Access Journals (Sweden)

    Marco Benoit Carbone

    2012-05-01

    Full Text Available Steven Poole is the author of Trigger Happy (2000. New York, NY: Arcade Publish, Unspeak (2006. New York, NY: Grove Press, and You Aren’t What You Eat (2012. In press. He has written extensively on books, culture, and videogames for The Guardian and other publications.

  8. Iron deficiency in chronic heart failure : An international pooled analysis

    NARCIS (Netherlands)

    Klip, IJsbrand T.; Comin-Colet, Josep; Voors, Adriaan A.; Ponikowski, Piotr; Enjuanes, Cristina; Banasiak, Waldemar; Lok, Dirk J.; Rosentryt, Piotr; Torrens, Ainhoa; Polonski, Lech; van Veldhuisen, Dirk J.; van der Meer, Peter; Jankowska, Ewa A.

    Background Iron deficiency (ID) is an emerging problem in patients with chronic heart failure (HF) and can be a potential therapeutic target. However, not much is known about the prevalence, predictors, and prognosis of ID in patients with chronic HF. Methods In an international pooled cohort

  9. Pooling data for the analysis of dynamic marketing systems

    NARCIS (Netherlands)

    Horvath, C.; Wieringa, J.E.

    Vector autoregressive (VAR) models have become popular in marketing literature for analyzing the behavior of competitive marketing systems. One drawback of these models is that the number of parameters can become very large, potentially leading to estimation problems. Pooling data for multiple

  10. Crowd Around: Expanding Your Donor Pool with Crowdfunding

    Science.gov (United States)

    Jarrell, Andrea

    2013-01-01

    At most institutions, annual fund-giving is down. Crowdfunding sites allow people with a great idea or worthy cause to bypass traditional funding methods and take their case directly to web-savvy investors and donors. This article describes how higher education institutions are expanding their donor pool through such crowdfunding sites as USEED,…

  11. Accounting Considerations in Public Sector Risk Management Pools.

    Science.gov (United States)

    Commons, Harriet V.

    1987-01-01

    The Government Accounting Standards Board's Insurance Issues Project has issued an invitation to comment on two issues: (1) whether governmental risk pools should follow the same accounting principles as commercial insurance companies and (2) financial statement disclosures required of entities with public accountability (MLF)

  12. Can Human Subject Pool Participation Benefit Sociology Students?

    Science.gov (United States)

    Chin, Lynn Gencianeo; Gibbs Stayte, Patricia

    2015-01-01

    Instructors at non-research institutions are less able to expose their students to research firsthand. Utilizing human subject pools (HSPs) in class may be a solution. Given that HSPs tend to be used in introduction to psychology classes at research institutions, we examine a community college HSP to answer three questions: (1) Do community…

  13. Stabilization of reactor fuel storage pool-TTP

    Energy Technology Data Exchange (ETDEWEB)

    Sevigny, G.

    1994-10-01

    The proposed work includes evaluating standard and improved technologies an designing an integrated demonstration system to clean the water and sludge the fuel storage pools. The water released would meet drinking water standards and tritium standards. The volume of radioactive sludge would be reduced by partial separation of the sludge and radionuclides and eventual solidification of the hazardous and radioactive waste. The scope of the wo includes a survey of needs and applicable technologies, system engineering evaluation, conceptual design, detailed design, fabrication of the integrat demonstration system, and testing of the system. The survey task will locate potential specific customers within the DOE complex, and outside of the DOE complex throughout the United States, that be able to utilize the narrowly focused technology to stabilize/shutdown reactor fuel storage pools, responsible parties will be located and asked respond to a survey about their specific process requirements. Literature searches will be run through technical and scientific databases to locate technologies that may be an improvement over the standard baselined technol for cleanup of radioactively-contaminated pools. Systems engineering will provide decision analysis support for the development, evaluation, design, test functions of the treatment of pool water and sludge.

  14. Southern Africa power pool : benefits, achievements, challenges and sustainability

    International Nuclear Information System (INIS)

    Anderson, G.O.; Opok, A.O.

    2008-01-01

    An overview of the Southern Africa Power Pool (SAPP) was presented. The SAPP was formed by 12 southern African developing countries as a means of minimizing electricity costs and maintaining reliability. The pool includes over 200 million people in a region of 9 million square meters. The SAPP relies on long-term bilateral contracts for electricity supply between customers and generators. The contracts are supplemented by short-term contracts in the short-term energy market. The pool uses a pricing mechanism with 13 detailed schedules dealing with firm power contracts of varying duration; non-firm power contracts of varying duration; mutual support contracts and emergency energy and control area services; scheduled outage energy; and energy banking. Benefits of the pool include reductions or postponements in generating capacity, reserves and fuel costs. New major initiatives planned by the SAPP include increasing interconnection points, the construction of new transmission lines, and the formulation of regional environmental studies. SAPP is now making plans to increase rural electrification in its membership countries with funding from an electricity levy. It was concluded that political and economic co-operation between member states has resulted in the sustained supply of energy in the region. 10 refs., 7 tabs., 3 figs

  15. Investigating the feasibility of municipal risk pooling as an ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Investigating the feasibility of municipal risk pooling as an adaptation finance measure. Climate change-related insurance products have been commonly used to manage the risks from climate hazards over the last few years. However, traditional insurance has not been effective for low-frequency, high-severity events.

  16. Convective cooling in a pool-type research reactor

    Energy Technology Data Exchange (ETDEWEB)

    Sipaun, Susan, E-mail: susan@nm.gov.my [Malaysian Nuclear Agency, Industrial Technology Division, Blok 29T, Bangi 43200, Selangor (Malaysia); Usman, Shoaib, E-mail: usmans@mst.edu [Missouri University of Science and Technology, Nuclear Engineering, 222 Fulton Hall 301 W.14th St., Rolla 64509 MO (United States)

    2016-01-22

    A reactor produces heat arising from fission reactions in the nuclear core. In the Missouri University of Science and Technology research reactor (MSTR), this heat is removed by natural convection where the coolant/moderator is demineralised water. Heat energy is transferred from the core into the coolant, and the heated water eventually evaporates from the open pool surface. A secondary cooling system was installed to actively remove excess heat arising from prolonged reactor operations. The nuclear core consists of uranium silicide aluminium dispersion fuel (U{sub 3}Si{sub 2}Al) in the form of rectangular plates. Gaps between the plates allow coolant to pass through and carry away heat. A study was carried out to map out heat flow as well as to predict the system’s performance via STAR-CCM+ simulation. The core was approximated as porous media with porosity of 0.7027. The reactor is rated 200kW and total heat density is approximately 1.07+E7 Wm{sup −3}. An MSTR model consisting of 20% of MSTR’s nuclear core in a third of the reactor pool was developed. At 35% pump capacity, the simulation results for the MSTR model showed that water is drawn out of the pool at a rate 1.28 kg s{sup −1} from the 4” pipe, and predicted pool surface temperature not exceeding 30°C.

  17. Theoretical assessment of particle generation from sodium pool fires

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, M., E-mail: monica.gmartin@ciemat.es [CIEMAT, Unit of Nuclear Safety Research, Av. Complutense, 40, 28040 Madrid (Spain); Herranz, L.E., E-mail: luisen.herranz@ciemat.es [CIEMAT, Unit of Nuclear Safety Research, Av. Complutense, 40, 28040 Madrid (Spain); Kissane, M.P., E-mail: Martin.KISSANE@oecd.org [Nuclear Safety Technology and Regulation Division, OECD Nuclear Energy Agency (NEA), 46 quai Alphonse Le Gallo, 92100 Boulogne-Billancourt (France)

    2016-12-15

    Highlights: • Development of particle generation model for sodium-oxides aerosol formation. • Development of partially validated numerical simulations to build up maps of saturation ratio. • Nucleation of supersaturated vapours as relevant source of aerosols over sodium pools. • Prediction of high concentrations of primary particles in the combustion zone. - Abstract: Potential sodium discharge in the containment during postulated Beyond Design Basis Accidents (BDBAs) in Sodium-cooled Fast Reactors (SFRs) would have major consequences for accident development in terms of energetics and source term. In the containment, sodium vaporization and subsequent oxidation would result in supersaturated oxide vapours that would undergo rapid nucleation creating toxic aerosols. Therefore, modelling this vapour nucleation is essential to proper source term assessment in SFRs. In the frame of the EU-JASMIN project, a particle generation model to calculate the particle generation rate and their primary size during an in-containment sodium pool fire has been developed. Based on a suite of individual models for sodium vaporization, oxygen natural circulation (3D modelling), sodium-oxygen chemical reactions, sodium-oxides-vapour nucleation and condensation, its consistency has been partially validated by comparing with available experimental data. As an outcome, large temperature and vapour concentration gradients set over the sodium pool have been found which result in large particle concentrations in the close vicinity of the pool.

  18. Initial surface deformations during impact on a liquid pool

    NARCIS (Netherlands)

    Bouwhuis, W.; Hendrix, M.H.W.; van der Meer, Roger M.; Snoeijer, Jacobus Hendrikus

    2015-01-01

    A tiny air bubble can be entrapped at the bottom of a solid sphere that impacts onto a liquid pool. The bubble forms due to the deformation of the liquid surface by a local pressure buildup inside the surrounding gas, as also observed during the impact of a liquid drop on a solid wall. Here, we

  19. Core neutronics of a swimming pool research reactor

    International Nuclear Information System (INIS)

    Mannan, M.A.; Mondal, M.A.W.; Pervini, M.E.

    1981-01-01

    The initial cores of the 5 MW swimming pool research reactor of the Nuclear Research Centre, Tehran have been analyzed using the computer codes METHUSELAH and EQUIPOISE. The effective multiplication factor, critical mass, moderator temperature and void coefficients of the core have been calculated and compared with vendor's values. Calculated values agree reasonably well with the vendor's results. (author)

  20. Epiphytic Algae study from pool of Ammiq (Bekaa, Lebanon)

    International Nuclear Information System (INIS)

    SLIM, K.

    1984-01-01

    In this particular place which constitutes the pool of Ammiq, 104 species and varieties have been collected. The diatoms constitute in themselves 85% of the algae population. This is an epiphytic microflora which is attached to the immerged macrophytics on this above mentioned place . (author)

  1. Reracking Possibilities of the NPP Krsko Spent Fuel Pool

    International Nuclear Information System (INIS)

    Bace, M.; Pevec, D.; Smuc, T.

    1998-01-01

    Using the SCALE-4 code package reracking possibilities of the NPP Krsko spent fuel pool were analyzed. Two cases were considered: the first case assuming the 40 years lifetime of the plant, and the second case assuming the 50 years lifetime of the plant. It was shown that it is possible to design the additional racks in free space of the spent fuel pool with the sufficient total capacity to store all the spent fuel generated during the 40 years lifetime of the plant. In the case of 50 years plant lifetime, completely new racks (capacity of 1890 spent fuel assemblies), containing 4mm boral in storage cell walls, were proposed for the NPP Krsko spent fuel pool. The effective multiplication factor of the spent fuel pool fully loaded with new racks containing spent fuel assemblies of initial enrichment 4.3 w/o, burned to 40 GWd/tU and cooled 2 years is lower than the value required by standard. It showed the possibility of the safe disposal of all spent fuel accumulated during more than 50 years lifetime of the plant. (author)

  2. 7 CFR 985.57 - Reserve pool requirements.

    Science.gov (United States)

    2010-01-01

    ... shall be paid by the equity holders. The proceeds from the disposition of reserve oil shall be... HANDLING OF SPEARMINT OIL PRODUCED IN THE FAR WEST Order Regulating Handling Volume Limitations § 985.57... the Secretary may establish, the Committee shall pool identified excess oil as reserve oil in such...

  3. Melt pool vorticity in deep penetration laser material welding

    Indian Academy of Sciences (India)

    weld pool has been evaluated in case of high power CO2 laser beam welding. The ... The experiments based on twin or triple spot interaction geometry have also ... while the other one is between the liquid and the solid states of the metal.

  4. Molten pool characterization of laser lap welded copper and aluminum

    Science.gov (United States)

    Xue, Zhiqing; Hu, Shengsun; Zuo, Di; Cai, Wayne; Lee, Dongkyun; Elijah, Kannatey-Asibu, Jr.

    2013-12-01

    A 3D finite volume simulation model for laser welding of a Cu-Al lap joint was developed using ANSYS FLUENT to predict the weld pool temperature distribution, velocity field, geometry, alloying element distribution and transition layer thickness—all key attributes and performance characteristics for a laser-welded joint. Melting and solidification of the weld pool was simulated with an enthalpy-porosity formulation. Laser welding experiments and metallographic examination by SEM and EDX were performed to investigate the weld pool features and validate the simulated results. A bowl-shaped temperature field and molten pool, and a unique maximum fusion zone width were observed near the Cu-Al interface. Both the numerical simulation and experimental results indicate an arch-shaped intermediate layer of Cu and Al, and a gradual transition of Cu concentration from the aluminum plate to the copper plate with high composition gradient. For the conditions used, welding with Cu on top was found to result in a better weld joint.

  5. Molten pool characterization of laser lap welded copper and aluminum

    International Nuclear Information System (INIS)

    Xue, Zhiqing; Hu, Shengsun; Zuo, Di; Cai, Wayne; Lee, Dongkyun; Elijah, Kannatey-Asibu Jr

    2013-01-01

    A 3D finite volume simulation model for laser welding of a Cu–Al lap joint was developed using ANSYS FLUENT to predict the weld pool temperature distribution, velocity field, geometry, alloying element distribution and transition layer thickness—all key attributes and performance characteristics for a laser-welded joint. Melting and solidification of the weld pool was simulated with an enthalpy-porosity formulation. Laser welding experiments and metallographic examination by SEM and EDX were performed to investigate the weld pool features and validate the simulated results. A bowl-shaped temperature field and molten pool, and a unique maximum fusion zone width were observed near the Cu–Al interface. Both the numerical simulation and experimental results indicate an arch-shaped intermediate layer of Cu and Al, and a gradual transition of Cu concentration from the aluminum plate to the copper plate with high composition gradient. For the conditions used, welding with Cu on top was found to result in a better weld joint. (paper)

  6. Validation of SPARC, a suppression pool aerosol capture model

    International Nuclear Information System (INIS)

    Owczarski, P.C.; Winegardner, W.K.

    1985-09-01

    A study of the potential for atmospheric release in hypothetical severe core melt accidents in BWRs with suppression pools was recently completed using a prototype of the SPARC code. The process of validating SPARC using an experimental data base is the concern of this paper

  7. Mitigating the impact of swimming pools on domestic water demand

    African Journals Online (AJOL)

    need to implement desalination schemes by ensuring water is used in in a 'fit for purpose' manner. This study therefore aims to better understand the impact that pools have on residential water demand through the analysis of metered water demand records and end-use modelling. The study site was the Liesbeek.

  8. On some aspects of Indian Ocean warm pool

    Digital Repository Service at National Institute of Oceanography (India)

    Saji, P.K.; Balchand, A.N.; RameshKumar, M.R.

    Annual and interannual variation of Indian Ocean Warm Pool (IOWP) was studied using satellite and in situ ocean temperature data IOWP surface area undergoes a strong annual cycle attaining a maximum of 24x106km2 during April...

  9. Afterlife of a Drop Impacting a Liquid Pool

    Science.gov (United States)

    Saha, Abhishek; Wei, Yanju; Tang, Xiaoyu; Law, Chung K.

    2017-11-01

    Drop impact on liquid pool is ubiquitous in industrial processes, such as inkjet printing and spray coating. While merging of drop with the impacted liquid surface is essential to facilitate the printing and coating processes, it is the afterlife of this merged drop and associated mixing which control the quality of the printed or coated surface. In this talk we will report an experimental study on the structural evolution of the merged droplet inside the liquid pool. First, we will analyze the depth of the crater created on the pool surface by the impacted drop for a range of impact inertia, and we will derive a scaling relation and the associated characteristic time-scale. Next, we will focus on the toroidal vortex formed by the moving drop inside the liquid pool and assess the characteristic time and length scales of the penetration process. The geometry of the vortex structure which qualitatively indicates the degree of mixedness will also be discussed. Finally, we will present the results from experiments with various viscosities to demonstrate the role of viscous dissipation on the geometry and structure formed by the drop. This work is supported by the Army Research Office and the Xerox Corporation.

  10. 47 CFR 52.20 - Thousands-block number pooling.

    Science.gov (United States)

    2010-10-01

    ... aligned with any particular telecommunication industry segment, and shall comply with the same neutrality... 47 Telecommunication 3 2010-10-01 2010-10-01 false Thousands-block number pooling. 52.20 Section 52.20 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES...

  11. The Ineffectiveness of Manual Treatment of Swimming Pools NNAJI ...

    African Journals Online (AJOL)

    Michael Horsfall

    there was a level of dissatisfaction among the swimmers. Some of ... period, the COD was above 80mg/l, the pH was between 6.2 and 7.1 as against 7.2 to 7.8 recommended by .... Fig 6: Chemical oxygen Demand of the Pool for Three Months.

  12. Convective cooling in a pool-type research reactor

    Science.gov (United States)

    Sipaun, Susan; Usman, Shoaib

    2016-01-01

    A reactor produces heat arising from fission reactions in the nuclear core. In the Missouri University of Science and Technology research reactor (MSTR), this heat is removed by natural convection where the coolant/moderator is demineralised water. Heat energy is transferred from the core into the coolant, and the heated water eventually evaporates from the open pool surface. A secondary cooling system was installed to actively remove excess heat arising from prolonged reactor operations. The nuclear core consists of uranium silicide aluminium dispersion fuel (U3Si2Al) in the form of rectangular plates. Gaps between the plates allow coolant to pass through and carry away heat. A study was carried out to map out heat flow as well as to predict the system's performance via STAR-CCM+ simulation. The core was approximated as porous media with porosity of 0.7027. The reactor is rated 200kW and total heat density is approximately 1.07+E7 Wm-3. An MSTR model consisting of 20% of MSTR's nuclear core in a third of the reactor pool was developed. At 35% pump capacity, the simulation results for the MSTR model showed that water is drawn out of the pool at a rate 1.28 kg s-1 from the 4" pipe, and predicted pool surface temperature not exceeding 30°C.

  13. Uniparental genetic heritage of belarusians: encounter of rare middle eastern matrilineages with a central European mitochondrial DNA pool.

    Science.gov (United States)

    Kushniarevich, Alena; Sivitskaya, Larysa; Danilenko, Nina; Novogrodskii, Tadeush; Tsybovsky, Iosif; Kiseleva, Anna; Kotova, Svetlana; Chaubey, Gyaneshwer; Metspalu, Ene; Sahakyan, Hovhannes; Bahmanimehr, Ardeshir; Reidla, Maere; Rootsi, Siiri; Parik, Jüri; Reisberg, Tuuli; Achilli, Alessandro; Hooshiar Kashani, Baharak; Gandini, Francesca; Olivieri, Anna; Behar, Doron M; Torroni, Antonio; Davydenko, Oleg; Villems, Richard

    2013-01-01

    Ethnic Belarusians make up more than 80% of the nine and half million people inhabiting the Republic of Belarus. Belarusians together with Ukrainians and Russians represent the East Slavic linguistic group, largest both in numbers and territory, inhabiting East Europe alongside Baltic-, Finno-Permic- and Turkic-speaking people. Till date, only a limited number of low resolution genetic studies have been performed on this population. Therefore, with the phylogeographic analysis of 565 Y-chromosomes and 267 mitochondrial DNAs from six well covered geographic sub-regions of Belarus we strove to complement the existing genetic profile of eastern Europeans. Our results reveal that around 80% of the paternal Belarusian gene pool is composed of R1a, I2a and N1c Y-chromosome haplogroups - a profile which is very similar to the two other eastern European populations - Ukrainians and Russians. The maternal Belarusian gene pool encompasses a full range of West Eurasian haplogroups and agrees well with the genetic structure of central-east European populations. Our data attest that latitudinal gradients characterize the variation of the uniparentally transmitted gene pools of modern Belarusians. In particular, the Y-chromosome reflects movements of people in central-east Europe, starting probably as early as the beginning of the Holocene. Furthermore, the matrilineal legacy of Belarusians retains two rare mitochondrial DNA haplogroups, N1a3 and N3, whose phylogeographies were explored in detail after de novo sequencing of 20 and 13 complete mitogenomes, respectively, from all over Eurasia. Our phylogeographic analyses reveal that two mitochondrial DNA lineages, N3 and N1a3, both of Middle Eastern origin, might mark distinct events of matrilineal gene flow to Europe: during the mid-Holocene period and around the Pleistocene-Holocene transition, respectively.

  14. Atlantic Warm Pool Trigger for the Younger Dryas Climate Event

    Science.gov (United States)

    Abdul, N. A.; Mortlock, R. A.; Wright, J. D.; Fairbanks, R. G.; Teneva, L. T.

    2011-12-01

    There is growing evidence that variability in the size and heat content of the tropical Atlantic Warm Pool impacts circum-North Atlantic climate via the Atlantic Multi-decadal Oscillation mode (Wang et al., 2008). The Atlantic Warm Pool spans the Gulf of Mexico, Caribbean Sea and the western tropical North Atlantic. Barbados is located near the center of the tropical Atlantic Warm Pool and coupled ocean models suggest that Barbados remains near the center of the tropical Atlantic Warm Pool under varying wind stress simulations. Measurements of the oxygen isotope paleothermometer in Acropora palmata coral species recovered from cores offshore Barbados, show a 3oC monotonic decrease in sea surface temperature from 13106 ± 83 to 12744 ± 61 years before present (errors given as 2 sigma). This interval corresponds to a sea level rise from 71.4 meters to 67.1 meters below present levels at Barbados. The 3oC temperature decrease is captured in eight A. palmata specimens that are in stratigraphic sequence, 230Th/234U dated, and analyzed for oxygen isotopes. All measurements are replicated. We are confident that this is the warm pool equivalent of the Younger Dryas climate event. The initiation of this temperature drop in the Atlantic Warm Pool predates the Younger Dryas start in Greenland ice cores, reported to start at 12896 ± 138 years (relative to AD 2000) (Rasmussen et al., 2006), while few other Younger Dryas climate records are dated with similar accuracy to make the comparison. Rasmussen, S.O., Andersen, K.K., Svensson, A.M., Steffensen, J.P., Vinther, B.M., Clausen, H.B., Siggaard-Andersen, M.L., Johnsen, S.J., Larsen, L.B., Dahl-Jensen, D., Bigler, M., Röthlisberger, R., Fischer, H., Goto-Azuma, K., Hansson, M.E., and Ruth, U., 2006, A new Greenland ice core chronology for the last glacial termination: J. Geophys. Res., v. 111, p. D06102. Wang, C., Lee, S.-K., and Enfield, D.B., 2008, Atlantic Warm Pool acting as a link between Atlantic Multidecadal

  15. Environmental controls of C, N and P biogeochemistry in peatland pools.

    Science.gov (United States)

    Arsenault, Julien; Talbot, Julie; Moore, Tim R

    2018-08-01

    Pools are common in northern peatlands but studies have seldom focused on their nutrient biogeochemistry, especially in relation to their morphological characteristics and through seasons. We determined the environmental characteristics controlling carbon (C), nitrogen (N) and phosphorus (P) biogeochemistry in pools and assessed their evolution over the course of the 2016 growing season in a subboreal ombrotrophic peatland of eastern Canada. We showed that water chemistry variations in 62 pools were significantly explained by depth (81.9%) and the surrounding vegetation type (14.8%), but not by pool area or shape. Shallow pools had larger dissolved organic carbon (DOC) and total nitrogen (TN) concentrations and lower pH than deep pools, while pools surrounded by coniferous trees had more recalcitrant DOC than pools where vegetation was dominated by mosses. The influence of depth on pool biogeochemistry was confirmed by the seasonal survey of pools of different sizes with 47.1% of the variation in pool water chemistry over time significantly explained. Of this, 67.3% was explained by the interaction between time and pool size and 32.7% by pool size alone. P concentrations were small in all pools all summer long and combined with high N:P ratios, are indicative of P-limitation. Our results show that pool biogeochemistry is influenced by internal processes and highlight the spatial and temporal heterogeneity of nutrient biogeochemistry in ombrotrophic peatlands. Copyright © 2018 Elsevier B.V. All rights reserved.

  16. Genome organization and expression of the rat ACBP gene family

    DEFF Research Database (Denmark)

    Mandrup, S; Andreasen, P H; Knudsen, J

    1993-01-01

    pool former. We have molecularly cloned and characterized the rat ACBP gene family which comprises one expressed and four processed pseudogenes. One of these was shown to exist in two allelic forms. A comprehensive computer-aided analysis of the promoter region of the expressed ACBP gene revealed...

  17. Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, B.A.; Phillips, S.J. [Jackson Lab., Bar Harbor, ME (United States)

    1996-06-15

    The inheritance of obesity has been analyzed in an intercross between the lean 129/Sv mouse strain and the obesity-prone EL/Suz mouse strain. The weights of three major fat pads were determined on 4-month-old mice, and the sum of these weights, divided by body weight, was used as an adiposity index. The strategy of selective DNA pooling was used as a primary screen to identify putative quantitative trait loci (QTLs) affecting adiposity index. DNA pools representing the leanest 15% and fattest 15% of the F2 progeny were compared for differential allelic enrichment using widely dispersed microsatellite variants. To evaluate putative QTLs, individual genotyping and interval mapping were employed to estimate QTL effects and assess statistical significance. One QTL affecting adiposity index, which accounted for 12.3% of phenotypic variance in gender-merged data, was mapped to the central region of Chromosome (Chr) 7. The QTL allele inherited from EL conferred increased adiposity. A second QTL that accounts for 6.3% of phenotypic variance was identified on Chr 1 near D1Mitt211. At both QTLs, the data are consistent with dominant inheritance of the allele contributing to obesity. The possible relationships between these QTLs and previously described obesity QYLs, major obesity mutations, and candidate genes are discussed. 42 refs., 3 figs., 3 tabs.

  18. Community analysis of plant biomass-degrading microorganisms from Obsidian Pool, Yellowstone National Park.

    Science.gov (United States)

    Vishnivetskaya, Tatiana A; Hamilton-Brehm, Scott D; Podar, Mircea; Mosher, Jennifer J; Palumbo, Anthony V; Phelps, Tommy J; Keller, Martin; Elkins, James G

    2015-02-01

    The conversion of lignocellulosic biomass into biofuels can potentially be improved by employing robust microorganisms and enzymes that efficiently deconstruct plant polysaccharides at elevated temperatures. Many of the geothermal features of Yellowstone National Park (YNP) are surrounded by vegetation providing a source of allochthonic material to support heterotrophic microbial communities adapted to utilize plant biomass as a primary carbon and energy source. In this study, a well-known hot spring environment, Obsidian Pool (OBP), was examined for potential biomass-active microorganisms using cultivation-independent and enrichment techniques. Analysis of 33,684 archaeal and 43,784 bacterial quality-filtered 16S rRNA gene pyrosequences revealed that archaeal diversity in the main pool was higher than bacterial; however, in the vegetated area, overall bacterial diversity was significantly higher. Of notable interest was a flooded depression adjacent to OBP supporting a stand of Juncus tweedyi, a heat-tolerant rush commonly found growing near geothermal features in YNP. The microbial community from heated sediments surrounding the plants was enriched in members of the Firmicutes including potentially (hemi)cellulolytic bacteria from the genera Clostridium, Anaerobacter, Caloramator, Caldicellulosiruptor, and Thermoanaerobacter. Enrichment cultures containing model and real biomass substrates were established at a wide range of temperatures (55-85 °C). Microbial activity was observed up to 80 °C on all substrates including Avicel, xylan, switchgrass, and Populus sp. Independent of substrate, Caloramator was enriched at lower (65 °C) temperatures.

  19. Suppression of the E. coli SOS response by dNTP pool changes.

    Science.gov (United States)

    Maslowska, Katarzyna H; Makiela-Dzbenska, Karolina; Fijalkowska, Iwona J; Schaaper, Roel M

    2015-04-30

    The Escherichia coli SOS system is a well-established model for the cellular response to DNA damage. Control of SOS depends largely on the RecA protein. When RecA is activated by single-stranded DNA in the presence of a nucleotide triphosphate cofactor, it mediates cleavage of the LexA repressor, leading to expression of the 30(+)-member SOS regulon. RecA activation generally requires the introduction of DNA damage. However, certain recA mutants, like recA730, bypass this requirement and display constitutive SOS expression as well as a spontaneous (SOS) mutator effect. Presently, we investigated the possible interaction between SOS and the cellular deoxynucleoside triphosphate (dNTP) pools. We found that dNTP pool changes caused by deficiencies in the ndk or dcd genes, encoding nucleoside diphosphate kinase and dCTP deaminase, respectively, had a strongly suppressive effect on constitutive SOS expression in recA730 strains. The suppression of the recA730 mutator effect was alleviated in a lexA-deficient background. Overall, the findings suggest a model in which the dNTP alterations in the ndk and dcd strains interfere with the activation of RecA, thereby preventing LexA cleavage and SOS induction. Published by Oxford University Press on behalf of Nucleic Acids Research 2015. This work is written by (a) US Government employee(s) and is in the public domain in the US.

  20. Measurements in large pool fires with an actively cooled calorimeter

    International Nuclear Information System (INIS)

    Koski, J.A.; Wix, S.D.

    1995-01-01

    The pool fire thermal test described in Safety Series 6 published by the International Atomic Energy Agency (IAEA) or Title 10, Code of Federal Regulations, Part 71 (10CFR71) in the United States is one of the most difficult tests that a container for larger ''Type B'' quantities of nuclear materials must pass. If retests of a container are required, costly redesign and project delays can result. Accurate measurements and modeling of the pool fire environment will ultimately lower container costs by assuring that containers past the pool fire test on the first attempt. Experiments indicate that the object size or surface temperature of the container can play a role in determining local heat fluxes that are beyond the effects predicted from the simple radiative heat transfer laws. An analytical model described by Nicolette and Larson 1990 can be used to understand many of these effects. In this model a gray gas represents soot particles present in the flame structure. Close to the container surface, these soot particles are convectively and radiatively cooled and interact with incident energy from the surrounding fire. This cooler soot cloud effectively prevents some thermal radiation from reaching the container surface, reducing the surface heat flux below the value predicted by a transparent medium model. With some empirical constants, the model suggested by Nicolette and Larson can be used to more accurately simulate the pool fire environment. Properly formulated, the gray gas approaches also fast enough to be used with standard commercial computer codes to analyze shipping containers. To calibrate this type of model, accurate experimental measurements of radiative absorption coefficients, flame temperatures, and other parameters are necessary. A goal of the calorimeter measurements described here is to obtain such parameters so that a fast, useful design tool for large pool fires can be constructed

  1. Pooling, meta-analysis, and the evaluation of drug safety

    Directory of Open Access Journals (Sweden)

    Leizorovicz Alain

    2002-03-01

    Full Text Available Abstract Background The "integrated safety report" of the drug registration files submitted to health authorities usually summarizes the rates of adverse events observed for a new drug, placebo or active control drugs by pooling the safety data across the trials. Pooling consists of adding the numbers of events observed in a given treatment group across the trials and dividing the results by the total number of patients included in this group. Because it considers treatment groups rather than studies, pooling ignores validity of the comparisons and is subject to a particular kind of bias, termed "Simpson's paradox." In contrast, meta-analysis and other stratified analyses are less susceptible to bias. Methods We use a hypothetical, but not atypical, application to demonstrate that the results of a meta-analysis can differ greatly from those obtained by pooling the same data. In our hypothetical model, a new drug is compared to 1 a placebo in 4 relatively small trials in patients at high risk for a certain adverse event and 2 an active reference drug in 2 larger trials of patients at low risk for this event. Results Using meta-analysis, the relative risk of experiencing the adverse event with the new drug was 1.78 (95% confidence interval [1.02; 3.12] compared to placebo and 2.20 [0.76; 6.32] compared to active control. By pooling the data, the results were, respectively, 1.00 [0.59; 1.70] and 5.20 [2.07; 13.08]. Conclusions Because these findings could mislead health authorities and doctors, regulatory agencies should require meta-analyses or stratified analyses of safety data in drug registration files.

  2. Energy Pooling Upconversion in Free Space and Optical Cavities

    Science.gov (United States)

    LaCount, Michael D.

    The ability to efficiently convert the wavelength of light has value in a wide range of disciplines that include the fields of photovoltaics, plant growth, optics and medicine. The processes by which such transformations are carried out are known as upconversions and downconversions. There are several ways to up/down convert light, each with its own attributes, issues, and competing mechanisms. Most are associated with one-body or two-body processes. Three-body dynamics are also possible though, going by the names of quantum cutting (downconversion) and energy pooling (upconversion). These use virtual excited electronic states to mediate conversions as has been experimentally realized using lanthanide ions embedded in wide bandgap materials. The use of lanthanides to convert light is not ideal due to their relative scarcity, toxicity, and the limited range of light frequencies that can be absorbed and emitted. Organic molecules, on the other hand, are typically non-toxic, are made up of abundant elements, and can be designed with tailored spectral properties. At issue is whether or not they can be used to carry out efficient energy pooling, the central question to be answered in this thesis. The research presented here draws on a perturbative quantum electrodynamics framework previously established for generic energy pooling. It was used to develop a computational methodology for determining the rate of energy pooling and its competing processes. This, in turn, draws on a combination of time-dependent density functional theory, quantum electrodynamics, and perturbation theory to generate the requisite material property data. This computational model was applied to two test systems consisting of stilbene-fluorescein and hexabenzocoronene-oligothiophene. The stilbene-fluorescein system was found to have a maximum energy pooling rate efficiency (as compared to competing processes) of 17% and the hexabenzocoronene-oligothiophene system was found to have a maximum

  3. Pharmacokinetics and pharmacogenetics of the MEK1/2 inhibitor, selumetinib, in Asian and Western healthy subjects: a pooled analysis.

    Science.gov (United States)

    Dymond, Angela W; Elks, Cathy; Martin, Paul; Carlile, David J; Mariani, Gabriella; Lovick, Susan; Huang, Yifan; Lorch, Ulrike; Brown, Helen; So, Karen

    2017-06-01

    Emerging data on selumetinib, a MEK1/2 inhibitor in clinical development, suggest a possible difference in pharmacokinetics (PK) between Japanese and Western patients. This pooled analysis sought to assess the effect of ethnicity on selumetinib exposure in healthy Western and Asian subjects, and to identify any association between genetic variants in the UGT1A1, CYP2C19 and ABCG2 genes and observed differences in selumetinib PK. A pooled analysis of data from ten Phase I studies, one in Asian subjects (encompassing Japanese, non-Japanese Asian and Indian Asian subjects) and nine in Western subjects, was conducted. Key findings were derived from the collective exposure data across doses of 25, 35, 50 and 75 mg selumetinib; primary variables were dose-normalized AUC and C max . PK data from 308 subjects (10 studies) were available for the pooled analysis; genetic data from 87 subjects (3 studies) were available for the pharmacogenetic analysis. Dose-normalized AUC and C max were 35% (95% CI: 25-47%) and 39% (95% CI: 24-56%) higher in the pooled Asian group, respectively, compared with Western subjects. PK exposure parameters were similar between the Japanese, non-Japanese Asian and Indian groups. There was no evidence that the polymorphisms assessed in the genes UGT1A1, CYP2C19 and ABCG2 account for observed PK differences. Selumetinib exposure was higher in healthy Asian subjects compared with Western subjects, and these data provide valuable insight for clinicians to consider when treating patients of Asian ethnicity with selumetinib.

  4. PIV measurements of turbulent jet and pool mixing produced by a steam jet discharge in a subcooled water pool

    International Nuclear Information System (INIS)

    Choo, Yeon Jun; Song, Chul-Hwa

    2010-01-01

    This experimental research is on the fluid-dynamic features produced by a steam injection into a subcooled water pool. The relevant phenomena could often be encountered in water cooled nuclear power plants. Two major topics, a turbulent jet and the internal circulation produced by a steam injection, were investigated separately using a particle image velocimetry (PIV) as a non-intrusive optical measurement technique. Physical domains of both experiments have a two-dimensional axi-symmetric geometry of which the boundary and initial conditions can be readily and well defined. The turbulent jet experiments with the upward discharging configuration provide the parametric values for quantitatively describing a turbulent jet such as the self-similar velocity profile, central velocity decay, spreading rate, etc. And in the internal circulation experiments with the downward discharging configuration, typical flow patterns in a whole pool region are measured in detail, which reveals both the local and macroscopic characteristics of the mixing behavior in a pool. This quantitative data on the condensing jet-induced mixing behavior in a pool could be utilized as benchmarking for a CFD simulation of relevant phenomena.

  5. New set of convective heat transfer coefficients established for pools and validated against CLARA experiments for application to corium pools

    Energy Technology Data Exchange (ETDEWEB)

    Michel, B., E-mail: benedicte.michel@irsn.fr

    2015-05-15

    Highlights: • A new set of 2D convective heat transfer correlations is proposed. • It takes into account different horizontal and lateral superficial velocities. • It is based on previously established correlations. • It is validated against recent CLARA experiments. • It has to be implemented in a 0D MCCI (molten core concrete interaction) code. - Abstract: During an hypothetical Pressurized Water Reactor (PWR) or Boiling Water Reactor (BWR) severe accident with core meltdown and vessel failure, corium would fall directly on the concrete reactor pit basemat if no water is present. The high temperature of the corium pool maintained by the residual power would lead to the erosion of the concrete walls and basemat of this reactor pit. The thermal decomposition of concrete will lead to the release of a significant amount of gases that will modify the corium pool thermal hydraulics. In particular, it will affect heat transfers between the corium pool and the concrete which determine the reactor pit ablation kinetics. A new set of convective heat transfer coefficients in a pool with different lateral and horizontal superficial gas velocities is modeled and validated against the recent CLARA experimental program. 155 tests of this program, in two size configurations and a high range of investigated viscosity, have been used to validate the model. Then, a method to define different lateral and horizontal superficial gas velocities in a 0D code is proposed together with a discussion about the possible viscosity in the reactor case when the pool is semi-solid. This model is going to be implemented in the 0D ASTEC/MEDICIS code in order to determine the impact of the convective heat transfer in the concrete ablation by corium.

  6. Controls on Filling and Evacuation of Sediment in Waterfall Plunge Pools

    Science.gov (United States)

    Scheingross, J. S.; Lamb, M. P.

    2014-12-01

    Many waterfalls are characterized by the presence of deep plunge pools that experience periods of sediment fill and evacuation. These cycles of sediment fill are a first order control on the relative magnitude of lateral versus vertical erosion at the base of waterfalls, as vertical incision requires cover-free plunge pools to expose the bedrock floor, while lateral erosion can occur when pools are partially filled and plunge-pool walls are exposed. Currently, there exists no mechanistic model describing sediment transport through waterfall plunge pools, limiting our ability to predict waterfall retreat. To address this knowledge gap, we performed detailed laboratory experiments measuring plunge-pool sediment transport capacity (Qsc_pool) under varying waterfall and plunge-pool geometries, flow hydraulics, and sediment size. Our experimental plunge-pool sediment transport capacity measurements match well with a mechanistic model we developed which combines existing waterfall jet theory with a modified Rouse profile to predict sediment transport capacity as a function of water discharge and suspended sediment concentration at the plunge-pool lip. Comparing the transport capacity of plunge pools to lower gradient portions of rivers (Qsc_river) shows that, for transport limited conditions, plunge pools fill with sediment under modest water discharges when Qsc_river > Qsc_pool, and empty to bedrock under high discharges when Qsc_pool > Qsc_river. These results are consistent with field observations of sand-filled plunge pools with downstream boulder rims, implying filling and excavation of plunge pools over single-storm timescales. Thus, partial filling of waterfall plunge pools may provide a mechanism to promote lateral undercutting and retreat of waterfalls in homogeneous rock in which plunge-pool vertical incision occurs during brief large floods that expose bedrock, whereas lateral erosion may prevail during smaller events.

  7. A common-pool resource experiment in acequia communities

    Directory of Open Access Journals (Sweden)

    Nejem Raheem

    2015-03-01

    Full Text Available Farmers and rural advocates in New Mexico assert that traditional irrigators are better adapted to water scarcity and variability than other communities. Data to actually test this are often scarce, but such information could be useful for planning the state’s water future, especially as climate change predictions tend toward less reliable supplies. This paper reports results from a common pool resource (CPR experiment that simulates irrigating behavior using two groups: rural irrigators and undergraduate students. Despite predictions to the opposite, there was no significant difference between mean withdrawals or predictions of other players’ behavior. On average, both groups withdrew above the social optimum but below the Nash equilibrium. This work appears to be the first example of a common pool resource experiment conducted with traditional New Mexican irrigators.

  8. Pool fire upon a balsa-filled shock absorber

    International Nuclear Information System (INIS)

    Fry, C.J.

    1990-07-01

    When performing a safety assessment of a transport flask with balsa-filled shock absorbers it is important to know how the shock absorbers, which may have the outer skin punctured by an impact, will perform in a fire. A 30 minute pool test, which satisfied all the requirements of a thermal test under the IAEA regulations, was carried out upon a small, balsa-filled shock absorber. The outer steel shell was partly cut away exposing the wood to the fire and the air. The balsa wood prevented 90% of the heat from the fire from being transferred through the shock absorber, even though the balsa was only 133 mm thick. The maximum heat flux through to the inside of the shock absorber due to the burning of the balsa wood was relatively low, 2.8 kW/m 2 , and occurred 2 to 3 hours after the end of the pool fire. (author)

  9. Steam CFD simulation of injection in suppression pool

    International Nuclear Information System (INIS)

    Naveen Samad, A.M.; Ghosh, Sumana

    2015-01-01

    Boiling water reactor (BWR) is one of the common types of electricity generating nuclear reactor. Suppression pool system is a major component of the BWR which has to be designed efficiently for the safe operations. During some accidents like Loss of Coolant Accident (LOCA) large amount of steam are injected to the pressure suppression system resulting in increase in temperature of the pool and thereby increasing the pressure. The present work discuss about the Computational Fluid Dynamics (CFD) simulation of steam injected to the wet well of BWR through the blow down pipes and there by investigating the hydrodynamic and thermal characteristics of the system. The simulations were carried out for three different steam injection velocities. The numerical simulations were performed with ANSYS FLUENT using multiphase 3D Volume of Fluid (VOF) model and k-ε model was adopted for modelling turbulence flow. (author)

  10. Modeling of air toxics from hydrocarbon pool fires

    International Nuclear Information System (INIS)

    Harvey, K.A.; Aydil, M.L.; Barone, J.B.

    1996-01-01

    While there is guidance for estimating the radiation hazards of fires (ARCHIE), there is little guidance on modeling the dispersion of hazardous materials from fires. The objective of this paper is to provide a review of the methodology used for modeling the impacts of liquid hydrocarbon pool fires. The required input variables for modeling of hydrocarbon pool fires include emission strength, emission duration, and dispersion characteristics. Methods for predicting the products of combustion including the use of literature values, test data, and thermodynamic equilibrium calculations are discussed. The use of energy balances coupled to radiative heat transfer calculations are presented as a method for determining flame temperature. Fire modeling literature is reviewed in order to determine other source release variables such as mass burn rate and duration and flame geometry

  11. Workshop on large molten pool heat transfer summary and conclusions

    International Nuclear Information System (INIS)

    1994-01-01

    The CSNI Workshop on Large Molten Heat Transfer held at Grenoble (France) in March 1994 was organised by CSNI's Principal Working Group on the Confinement of Accidental Radioactive Releases (PWG4) with the cooperation of the Principal Working Group on Coolant System Behaviour (FWG2) and in collaboration with the Grenoble Nuclear Research Centre of the French Commissariat a l'Energie Atomique (CEA). Conclusions and recommendations are given for each of the five sessions of the workshops: Feasibility of in-vessel core debris cooling through external cooling of the vessel; Experiments on molten pool heat transfer; Calculational efforts on molten pool convection; Heat transfer to the surrounding water - experimental techniques; Future experiments and ex-vessel studies (open forum discussion)

  12. Numerical analysis on pool boiling using user defined function

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, Sung Uk; Jeon, Byong Guk; Kim, Seok; Euh, Dong-Jin [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    PAFS (passive auxiliary feedwater system) adopted in the APR+ (Advanced Power Reactor Plus) of Korea is one such application. When PAFS is activated with an actuation signal, steam from the steam generator passes through heat exchanger tubes submerged in a water tank of the PAFS. Outside these heat exchanger tubes, nucleate boiling phenomena appears. In the present work, a numerical study is reported on three-dimensional transient state pool boiling of water having an immersed heat source. The velocity vector fields during the decrease in the water level are numerically investigated in a pool, and the accuracy of the results is checked by comparing the experimental results conducted using the PIV techniques by Kim et al. These numerical results can be used as basic research data for an analysis and prediction of the natural circulation phenomena in the cooling tank of the passive safety system in a nuclear power plant.

  13. Streaming Pool: reuse, combine and create reactive streams with pleasure

    CERN Multimedia

    CERN. Geneva

    2017-01-01

    When connecting together heterogeneous and complex systems, it is not easy to exchange data between components. Streams of data are successfully used in industry in order to overcome this problem, especially in the case of "live" data. Streams are a specialization of the Observer design pattern and they provide asynchronous and non-blocking data flow. The ongoing effort of the ReactiveX initiative is one example that demonstrates how demanding this technology is even for big companies. Bridging the discrepancies of different technologies with common interfaces is already done by the Reactive Streams initiative and, in the JVM world, via reactive-streams-jvm interfaces. Streaming Pool is a framework for providing and discovering reactive streams. Through the mechanism of dependency injection provided by the Spring Framework, Streaming Pool provides a so called Discovery Service. This object can discover and chain streams of data that are technologically agnostic, through the use of Stream IDs. The stream to ...

  14. Suppression Pools: paradigm of the thermalhydraulic effect on severe accidents

    International Nuclear Information System (INIS)

    Herranz, L. E.; Lopez del Pra, C.

    2016-01-01

    Influence of thermal-hydrualic phenomena on severe accident unforlding is beyond question. The present paper supports this statement on two key aspects of a severe accident: preservation of containment integrity and transport of fission products once released from fuel. To illustrate them, the attention is focused on suppression pools performance and, particularly, on some recent findings stemming from authors research of Fukushima scenarios. Gas behvaior at the injection point and its later evolution, potential axial and/or azimuthal stratification of the aqueous body or water saturation state, are some of the processes tha more strongly affect the role of pools as a mass and energy sink. They are described and discussed in detail. (Author)

  15. Two dimensional polar display of cardiac blood pool SPECT

    International Nuclear Information System (INIS)

    Honda, Norinari; Machida, Kikuo; Mamiya, Toshio; Takahashi, Taku; Takishima, Teruo; Hasegawa, Noriko; Hashimoto, Masanori; Ohno, Ken

    1989-01-01

    A new method of ECG gated cardiac blood pool SPECT to illustrate the left ventricular (LV) wall motion in a single static image, two dimensional polar display (2DPD), was described. Circumferential profiles of the difference between end diastolic and end systolic short axis images of the LV were displayed in a similar way to the bull's eye plot of 201 Tl myocardial SPECT. The diagnoses by 2DPDs agreed with those by cinematic displays of ECG gated blood pool SPECT in 74 out of 84 segments (85.5%) of abnormal motion, and 155 out of 168 segments (80.3%) of normal motion. It is concluded that 2DPD can evaluate regional wall motion by a single static image in a significant number of patients, and is also useful in comparing with the bull's eye image of 201 Tl myorcardial SPECT. (orig.)

  16. LCG Persistency Framework (CORAL, COOL, POOL): Status and Outlook

    International Nuclear Information System (INIS)

    Valassi, A; Kalkhof, A; Loth, A; Trentadue, R; Clemencic, M; Frank, M; Pokorski, W; Dykstra, D; Front, D; Govi, G; Nowak, M; Salnikov, A; Schmidt, S A; Wache, M; Xie, Z

    2011-01-01

    The Persistency Framework consists of three software packages (CORAL, COOL and POOL) addressing the data access requirements of the LHC experiments in different areas. It is the result of the collaboration between the CERN IT Department and the three experiments (ATLAS, CMS and LHCb) that use this software to access their data. POOL is a hybrid technology store for C++ objects, metadata catalogs and collections. CORAL is a relational database abstraction layer with an SQL-free API. COOL provides specific software tools and components for the handling of conditions data. This paper reports on the status and outlook of the project and reviews in detail the usage of each package in the three experiments.

  17. Pressure wave propagation in the discharge piping with water pool

    International Nuclear Information System (INIS)

    Bang, Young S.; Seul, Kwang W.; Kim, In Goo

    2004-01-01

    Pressure wave propagation in the discharge piping with a sparger submerged in a water pool, following the opening of a safety relief valve, is analyzed. To predict the pressure transient behavior, a RELAP5/MOD3 code is used. The applicability of the RELAP5 code and the adequacy of the present modeling scheme are confirmed by simulating the applicable experiment on a water hammer with voiding. As a base case, the modeling scheme was used to calculate the wave propagation inside a vertical pipe with sparger holes and submerged within a water pool. In addition, the effects on wave propagation of geometric factors, such as the loss coefficient, the pipe configuration, and the subdivision of sparger pipe, are investigated. The effects of inflow conditions, such as water slug inflow and the slow opening of a safety relief valve are also examined

  18. Peculiarities of natural convective heat removal from complex pools

    International Nuclear Information System (INIS)

    Groetzbach, Guenther

    2002-01-01

    Considerable sensitivities are investigated in using natural convection for cooling large pools. Such a flow occurred in a sump cooling concept for a water cooled reactor. The related SUCOS model experiments were analyzed by means of the FLUTAN code. The numerical interpretations show, the natural convection in large pools is strongly influenced by local thermal disturbances, either due to structures in the fluid domain, or by bounding structures interacting thermally with the fluid. These experiment specific disturbances must be recorded in the numerical model in order to achieve adequate simulations of the heat transport. Some geometric imperfections of horizontal coolers or heaters could also have tremendous influences. As a consequence, not only the numerical model has to record all relevant phenomena as realistic as possible, but also the model experiment. (author)

  19. Biofouling on austenitic stainless steels in spent nuclear fuel pools

    Energy Technology Data Exchange (ETDEWEB)

    Sarro, M I; Moreno, D A; Chicote, E; Lorenzo, P I; Garcia, A M [Universidad Politecnica de Madrid, Departamento de Ingenieria y Ciencia de los Materiales, Escuela Tecnica Superior de Ingenieros Industriales, Jose Gutierrez Abascal, 2, E-28006 Madrid (Spain); Montero, F [Iberdrola Generacion, S.A., y C.M.D.S., Centro de Tecnologia de Materiales, Paseo de la Virgen del Puerto, 53, E-28005 Madrid (Spain)

    2003-07-01

    The objective of this study was to investigate the biofilm formation on three different types of austenitic stainless steel (UNS S30400, S30466 and S31600) submerged in a spent nuclear fuel pool. The presence of microorganisms in coupons was characterised using standard culture microbiological methods, microscopic techniques (epifluorescence microscopy and scanning electron microscopy), and molecular biology techniques (denaturing gradient gel electrophoresis and sequencing fragments of 16S rDNA). The microscopy techniques showed signs of colonisation of stainless steels in spite of these extreme conditions. Based on sequencing of cultured microorganisms, different bacteria belonging to {alpha}, {beta}, {gamma}-Proteobacteria, Bacilli, and Actinobacteria classes have been identified. The biofilm radioactivity was measured using gamma-ray spectrometry and, according to the data gathered, the radionuclides present in the water pool were entrapped in the biofilm increasing the amount of radiation at the surface of the different materials. (Abstract Copyright [2003], Wiley Periodicals, Inc.)

  20. Biofouling on austenitic stainless steels in spent nuclear fuel pools

    International Nuclear Information System (INIS)

    Sarro, M.I.; Moreno, D.A.; Chicote, E.; Lorenzo, P.I.; Garcia, A.M.; Montero, F.

    2003-01-01

    The objective of this study was to investigate the biofilm formation on three different types of austenitic stainless steel (UNS S30400, S30466 and S31600) submerged in a spent nuclear fuel pool. The presence of microorganisms in coupons was characterised using standard culture microbiological methods, microscopic techniques (epifluorescence microscopy and scanning electron microscopy), and molecular biology techniques (denaturing gradient gel electrophoresis and sequencing fragments of 16S rDNA). The microscopy techniques showed signs of colonisation of stainless steels in spite of these extreme conditions. Based on sequencing of cultured microorganisms, different bacteria belonging to α, β, γ-Proteobacteria, Bacilli, and Actinobacteria classes have been identified. The biofilm radioactivity was measured using gamma-ray spectrometry and, according to the data gathered, the radionuclides present in the water pool were entrapped in the biofilm increasing the amount of radiation at the surface of the different materials. (Abstract Copyright [2003], Wiley Periodicals, Inc.)