WorldWideScience

Sample records for fsh habitat enhancement

  1. Umatilla River Basin Anadromous Fsh Habitat Enhancement Project : 2000 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Shaw, R. Todd

    2001-12-31

    The Umatilla River Basin Anadromous Fish Habitat Enhancement Project continued to identify impacted stream reaches throughout the Umatilla River Basin for habitat improvements during the 2000 project period. Public outreach efforts, biological and physical monitoring, and continued development of a Umatilla River Basin Watershed Assessment assisted the project in fostering public cooperation, targeting habitat deficiencies and determining habitat recovery measures. Habitat enhancement projects continued to be maintained on 44 private properties, four riparian easements and one in-stream enhancement agreement were secured, two new projects implemented and two existing projects improved to enhance anadromous fish habitat and natural fisheries production capabilities in the Umatilla River Basin. New project locations included sites on the mid Umatilla River and Buckaroo Creek. Improvements were implemented at existing project sites on the upper Umatilla River and Wildhorse Creek. A stream bank stabilization project was implemented at approximately River Mile 37.4 Umatilla River to stabilize 760 feet of eroding stream bank and improve in-stream habitat diversity. Habitat enhancements at this site included construction of six rock barbs with one large conifer root wad incorporated into each barb, stinging approximately 10,000 native willow cuttings, planting 195 tubling willows and 1,800 basin wildrye grass plugs, and seeding 40 pounds of native grass seed. Staff time to assist in development of a subcontract and fence materials were provided to establish eight spring sites for off-stream watering and to protect wetlands within the Buckaroo Creek Watershed. A gravel bar was moved and incorporated into an adjacent point bar to reduce stream energy and stream channel confinement within the existing project area at River Mile 85 Umatilla River. Approximately 10,000 native willow cuttings were stung and trenched into the stream channel margins and stream banks, and 360

  2. Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats

    OpenAIRE

    2014-01-01

    An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin F2α (PGF2α). The pFSH tre...

  3. Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats.

    Science.gov (United States)

    Rahman, M R; Rahman, M M; Wan Khadijah, W E; Abdullah, R B

    2014-09-01

    An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin F2α (PGF2α). The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily). Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (pembryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

  4. Follicle Stimulating Hormone (FSH Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats

    Directory of Open Access Journals (Sweden)

    M. R. Rahman

    2014-09-01

    Full Text Available An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH dosage based on body weight (BW on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR device and a single injection of prostaglandin F2α (PGF2α. The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily. Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05 were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05 in pFSH-5 (7.00 and 6.71 than pFSH-3 (3.00 and 2.80 and pFSH-8 (2.00 and 1.50, respectively. In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

  5. Salmon River Habitat Enhancement, 1989 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Rowe, Mike

    1989-04-01

    This project was funded by the Bonneville Power Administration (BPA). The annual report contains three individual subproject papers detailing tribal fisheries work completed during the summer and fall of 1989. Subproject 1 contains summaries of evaluation/monitoring efforts associated with the Bear Valley Creek, Idaho enhancement project. Subproject 2 contains an evaluation of the Yankee Fork of the Salmon River habitat enhancement project. This report has been sub-divided into two parts: Part 1; stream evaluation and Part 2; pond series evaluation. Subproject 3 concerns the East Fork of the Salmon River, Idaho. This report summarizes the evaluation of the project to date including the 1989 pre-construction evaluation conducted within the East Fork drainage. Dredge mining has degraded spawning and rearing habitat for chinook salmon and steelhead trout in the Yankee Fork drainage of the Salmon River and in Bear Valley Creek. Mining, agricultural, and grazing practices degraded habitat in the East Fork of the Salmon River. Biological monitoring of the success of habitat enhancement for Bear Valley Creek and Yankee Fork are presented in this report. Physical and biological inventories prior to habitat enhancement in East Fork were also conducted. Four series of off-channel ponds of the Yankee Fork are shown to provide effective rearing habitat for chinook salmon. 45 refs., 49 figs., 24 tabs.

  6. Salmon River Habitat Enhancement, 1984 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Konopacky, Richard C.

    1986-04-01

    This report has four volumes: a Tribal project annual report (Part 1) and three reports (Parts 2, 3, and 4) prepared for the Tribes by their engineering subcontractor. The Tribal project annual report contains reports for four subprojects within Project 83-359. Subproject I involved habitat and fish inventories in Bear Valley Creek, Valley County, Idaho that will be used to evaluate responses to ongoing habitat enhancement. Subproject II is the coordination/planning activities of the Project Leader in relation to other BPA-funded habitat enhancement projects that have or will occur within the traditional Treaty (Fort Bridger Treaty of 1868) fishing areas of the Shoshone-Bannock Tribes, Fort Hall Reservation, Idaho. Subproject III involved habitat and fish inventories (pretreatment) and habitat problem identification on the Yankee Fork of the Salmon River (including Jordan Creek). Subproject IV during 1985 involved habitat problem identification in the East Fork of the Salmon River and habitat and fish inventories (pretreatment) in Herd Creek, a tributary to the East Fork.

  7. Salmon River Habitat Enhancement. 1990 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Rowe, Mike

    1991-12-01

    The annual report contains three individual subproject sections detailing tribal fisheries work completed during the summer and fall of 1990. Subproject I contains summaries of evaluation/monitoring efforts associated with the Bear Valley Creek, Idaho enhancement project. Subproject II contains an evaluation of the Yankee Fork of the Salmon River habitat enhancement project. Subproject III concerns the East Fork of the Salmon River, Idaho.

  8. Fox Island Division habitat rehabilitation and enhancement project

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The goal of this Habitat Rehabilitation and Enhancement Project (HREP) is to rehabilitate and enhance aquatic and related floodplain habitat. The purpose of this...

  9. Enhancements of the "eHabitat

    Science.gov (United States)

    Santoro, M.; Dubois, G.; Schulz, M.; Skøien, J. O.; Nativi, S.; Peedell, S.; Boldrini, E.

    2012-04-01

    standard discovery service; b) A Discovery Augmentation Component (DAC): this component builds on existing discovery and semantic services in order to provide the infrastructure with semantics enabled queries; c) A Data Access Broker: this component provides a seamless access of heterogeneous remote resources via a unique and standard service; d) Environmental Modeling Components (i.e. OGC WPS): these implement algorithms to predict evolution of protected areas This presentation introduces the advanced infrastructure developed to enhance the "eHabitat" use scenario. The presented infrastructure will be accessible through the GEO Portal and was used for demonstrating the "eHabitat" model at the last GEO Plenary Meeting - Istanbul, November 2011.

  10. Napa River Sediment TMDL Implementation and Habitat Enhancement Project

    Science.gov (United States)

    Information about the SFBWQP Napa River Sediment TMDL Implementation and Habitat Enhancement Project, part of an EPA competitive grant program to improve SF Bay water quality focused on restoring impaired waters and enhancing aquatic resources.

  11. Modeling predator habitat to enhance reintroduction planning

    Science.gov (United States)

    Shiloh M. Halsey; William J. Zielinski; Robert M. Scheller

    2015-01-01

    Context The success of species reintroduction often depends on predation risk and spatial estimates of predator habitat. The fisher (Pekania pennanti) is a species of conservation concern and populations in the western United States have declined substantially in the last century. Reintroduction plans are underway, but the ability...

  12. Clackamas/Hood River Habitat Enhancement Program, 1987 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    MacDonald, Ken (Mount Hood National Forest, Hood River Ranger District, Parkdale, OR); Cain, Thomas C. (Mount Hood National Forest, Clackamas River Ranger District, Estacada, OR); Heller, David A. (Mount Hood National Forest, Gresham, OR)

    1988-03-01

    Fisheries habitat improvement work is being done on priority drainages in the Clackamas and Rood River sub-basins under program measure 704(c), Action Item 4.2 of the Northwest Power Planning Council's Columbia River Basin Fish and Wildlife Program. This report describes the work completed in 1987 for Bonneville Power Administration (BPA) project number 84-11, the Clackamas/Hood River Habitat Enhancement Program. The program is composed of six projects: Collawash River Habitat Improvement Project; Collawash River Falls Passage Improvement Project, Oak Grove Fork Habitat Improvement Project; Lake Branch/West Fork Hood River Habitat Improvement Project; Fifteenmile Creek Habitat Improvement Project; and Abundance, Behavior, and Habitat Utilization by Coho Salmon and Steelhead Trout in Fish Creek, Oregon, As Influenced by Habitat Enhancement. This ongoing program was initiated in 1984, although some of the projects were begun with BPA funding support as early as 1983. The projects are complemented by a variety of habitat improvement and management activities funded from a variety of Forest Service sources. This report describes the activities implemented for five of the six projects. A separate annual report on the 1987 habitat improvement and monitoring/evaluation efforts in the Fish Creek drainage has been prepared. Species for management emphasis include spring chinook and coho salmon, and summer and winter steelhead trout. Project work in 1987 primarily focused on increasing the quantity and quality of available rearing habitat, and improving access at passage barriers. The underlying theme of the improvement work has been to increase habitat diversity through the introduction of ''structure''. Structure provided by logs and boulders serves to deflect, pond, or otherwise disrupt flow patterns within a stream channel. This alteration of flow patterns results in formation of an increased number of habitat niches (i.e. pools, glides, alcoves

  13. Duck Valley Habitat Enhancement and Protection, 2000 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Dodson, Guy; Pero, Vincent (Shoshone-Paiute Nation, Duck Valley Indian Reservation, Owyhee, NV)

    2000-01-01

    The Duck Valley Indian Reservations' Habitat Enhancement project is an ongoing project designed to enhance and protect the critical riparian areas, natural springs, and native fish spawning areas on the Reservation. The project was begun in 1997 with the hiring of a fisheries biologist and the creation of a new department for the Tribes. The project's goals are to protect and enhance the springs, Owyhee River, its tributaries, and to develop a database that can be used by other fisheries professionals which includes information on water quality and fish composition, health, abundance, and genetic makeup. One habitat portion of the project is a focus on protection the numerous springs that provide clean, cool water to the Owyhee River. This will be accomplished through enclosure fences of the spring heads and water troughs to provide clean cool drinking water for wildlife and livestock. Another habitat portion of the project involves protecting headwater areas of streams with native fish populations. This is accomplished through enclosure fencing and riparian plantings on any eroded or degraded banks in the enclosure area. Finally, we monitor and evaluate the areas protected and enhanced. This is accomplished through biological sampling for temperature, Oxygen, sedimentation, and measurements of water depth, bank height and undercut, and width of stream. With the habitat and biological indices we will be able to evaluate how well protective measures are doing, and where to focus future efforts.

  14. Enhancing and restoring habitat for the desert tortoise (Gopherus agassizii)

    Science.gov (United States)

    Abella, Scott R.; Berry, Kristin H.

    2016-01-01

    roads. Habitats will not necessarily recover naturally after severe disturbance and from centuries of degradation. A next step in recovering tortoise habitats could be implementing a set of integrated habitat enhancements (e.g., reducing non-native plants, improving forage quality, augmenting native perennial plants, and ameliorating altered hydrology) and monitoring short- and long-term indicators of habitat and tortoise health. This synthesis supports implementation of habitat enhancement measures described in the U.S. Fish and Wildlife Service's 2011 Revised Recovery Plan for the desert tortoise.

  15. Grande Ronde Basin Fish Habitat Enhancement Project : 2000 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    McGowan, Vance R.; Powell, Russ M.; Stennfeld, Scott P.

    2001-04-01

    On July 1, 1984 the Bonneville Power Administration and the Oregon Department of Fish and Wildlife entered into an agreement to initiate fish habitat enhancement work in the Joseph Creek subbasin of the Grande Ronde River Basin in northeast Oregon. In July of 1985 the Upper and Middle Grande Ronde River, and Catherine Creek subbasins were included in the intergovernmental contract, and on March 1, 1996 the Wallowa River subbasin was added. The primary goal of ''The Grande Ronde Basin Fish Habitat Enhancement Project'' is to access, create, improve, protect, and restore riparian and instream habitat for anadromous salmonids, thereby maximizing opportunities for natural fish production within the basin. This project provided for implementation of Program Measure 703 (C)(1), Action Item 4.2 of the Northwest Power Planning Council's Columbia River Basin Fish and Wildlife Program (NPPC, 1987), and continues to be implemented as offsite mitigation for mainstem fishery losses caused by the Columbia River hydro-electric system. All work conducted by the Oregon Department of Fish and Wildlife is on private lands and therefore requires that considerable time be spent developing rapport with landowners to gain acceptance of, and continued cooperation with this program throughout 10-15 year lease periods. This project calls for passive regeneration of habitat, using riparian enclosure fencing as the primary method to restore degraded streams to a normative condition. Active remediation techniques using plantings, off-site water developments, site-specific instream structures, or whole channel alterations are also utilized where applicable. Individual projects contribute to and complement ecosystem and basin-wide watershed restoration efforts that are underway by state, federal, and tribal agencies, and local watershed councils. Work undertaken during 2000 included: (1) Implementing 2 new projects in the Grande Ronde drainage, and retrofitting one old

  16. Fifteenmile Basin Habitat Enhancement Project: Annual Report FY 1988.

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Roger C.; Marx, Steven D.

    1989-04-01

    The goal of the Fifteenmile Creek Habitat Enhancement Project is to improve wild winter steelhead in the Fifteenmile Creek Basin under the Columbia River Basin Fish and Wildlife Program. The project is funded by through the Bonneville Power Administration. Cooperators in the habitat enhancement project include the USDA Forest Service, Wasco County Soil and Water Conservation District and the Confederated Tribes of the Warms Springs. Installation of instream fish habitat structures was completed on four miles of Ramsey Creek and on one mile of Fifteenmile Creek. One hundred thirty-five structures were installed in treatment areas. Construction materials included logs and rock. Riparian protection fencing was completed on Dry Creek and Ramsey Creek worksites. Five and one-half miles of new fence was added to existing fence on Ramsey Creek to afford riparian protection to four miles of stream. Six miles of stream on Dry Creek will be afforded riparian protection by constructing 4.5 miles of fence to complement existing fence. 2 refs., 5 figs.

  17. Grande Ronde Basin Fish Habitat Enhancement Project : 2007 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    McGowan, Vance R.; Morton, Winston H.

    2008-12-30

    On July 1, 1984 the Bonneville Power Administration and the Oregon Department of Fish and Wildlife entered into an intergovernmental contract to initiate fish habitat enhancement work in the Joseph Creek subbasin of the Grande Ronde River Basin in northeast Oregon. In 1985 the Upper and Middle Grande Ronde River, and Catherine Creek subbasins were included in the contract, and in 1996 the Wallowa River subbasin was added. The primary goal of 'The Grande Ronde Basin Fish Habitat Enhancement Project' is to create, protect, and restore riparian and instream habitat for anadromous salmonids, thereby maximizing opportunities for natural fish production within the basin. This project provided for implementation of Program Measure 703 (C)(1), Action Item 4.2 of the Northwest Power Planning Council's Columbia River Basin Fish and Wildlife Program (NPPC, 1987), and continues to be implemented as offsite mitigation for mainstem fishery losses caused by the Columbia River hydro-electric system. All work conducted by the Oregon Department of Fish and Wildlife and partners is on private lands and therefore requires that considerable time be spent developing rapport with landowners to gain acceptance of, and continued cooperation with this program throughout 10-15 year lease periods. Both passive and active restoration treatment techniques are used. Passive regeneration of habitat, using riparian exclosure fencing and alternate water sources are the primary method to restore degraded streams when restoration can be achieved primarily through changes in management. Active restoration techniques using plantings, bioengineering, site-specific instream structures, or whole stream channel alterations are utilized when streams are more severely degraded and not likely to recover in a reasonable timeframe. Individual projects contribute to and complement ecosystem and basin-wide watershed restoration efforts that are underway by state, federal, and tribal agencies, and

  18. Effects of Habitat Enhancement on Steelhead Trout and Coho Salmon Smolt Production, Habitat Utilization, and Habitat Availability in Fish Creek, Oregon, 1986 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Everest, Fred H.; Reeves, Gordon H. (Oregon State University, Pacific Northwest Forest and Range Experiment Station, Corvallis, OR); Hohler, David B. (Mount Hood National Forest, Clackamas River Ranger District, Estacada, OR)

    1987-06-01

    Construction and evaluation of salmonid habitat improvements on Fish Creek, a tributary of the upper Clackamas River, was continued in fiscal year 1986 by the Estacada Ranger District, Mt. Hood National Forest, and the Anadromous Fish Habitat Research Unit of the Pacific Northwest Research Station (PNW), USDA Forest Service. The study began in 1982 when PNW entered into an agreement with the Mt. Hood National Forest to evaluate fish habitat improvements in the Fish Creek basin on the Estacada Ranger District. The project was initially conceived as a 5-year effort (1982-1986) to be financed with Forest Service funds. The habitat improvement program and the evaluation of improvements were both expanded in mid-1983 when the Bonneville Power Administration (BPA) entered into an agreement with the Mt. Hood National Forest to cooperatively fund work on Fish Creek. Habitat improvement work in the basin is guided by the Fish Creek Habitat Rehabilitation-Enhancement Framework developed cooperatively by the Estacada Ranger District, the Oregon Department of Fish and Wildlife, and the Pacific Northwest Research Station (see Appendix 2). The framework examines potential factors limiting production of salmonids in the basin, and the appropriate habitat improvement measures needed to address the limiting factors. Habitat improvement work in the basin has been designed to: (1) improve quantity, quality, and distribution of spawning habitat for coho and spring chinook salmon and steelhead trout, (2) increase low flow rearing habitat for steelhead trout and coho salmon, (3) improve overwintering habitat for coho salmon and steelhead trout, (4) rehabilitate riparian vegetation to improve stream shading to benefit all species, and (5) evaluate improvement projects from a drainage wide perspective. The objectives of the evaluation include: (1) Drainage-wide evaluation and quantification of changes in salmonid spawning and rearing habitat resulting from a variety of habitat

  19. Habitat Restoration/Enhancement Fort Hall Reservation : 2008 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Osborne, Hunter [Shoshone Bannock Tribes

    2009-07-23

    Habitat enhancement, protection and monitoring were the focus of the Resident Fisheries Program during 2008. Enhancement and protection included sloping, fencing and planting wetlands plugs at sites on Spring Creek (Head-waters). Many previously constructed instream structures (rock barbs and wing dams) were repaired throughout the Fort Hall Indian Reservation (Reservation). Physical sampling during 2008 included sediment and depth measurements (SADMS) in Spring Creek at the Car Removal site. SADMS, used to track changes in channel morphology and specifically track movements of silt through Bottoms stream systems were completed for 5 strata on Spring Creek. Water temperature and chemistry were monitored monthly on Spring Creek, Clear Creek, Diggie Creek, and Portneuf (Jimmy Drinks) and Blackfoot rivers. Fish population densities and biomass were sampled in five reservation streams which included nine sites. Sampling protocols were identical to methods used in past years. Numbers of fish in Spring Creek series remained relatively low, however, there was an increase of biomass overall since 1993. Salmonid fry densities were monitored near Broncho Bridge and were similar to 2006, and 2007, however, as in years past, high densities of macrophytes make it very difficult to see fry in addition to lack of field technicians. Mean catch rate by anglers on Bottoms streams stayed the same as 2007 at 1.5/hr. Numbers of fish larger than 18-inches caught by anglers increased from 2007 at .20 to .26/hr.

  20. Grande Ronde Basin Fish Habitat Enhancement Project : 1998 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    McGowan, Vance R.; Powell, Russ M.

    1999-05-01

    The primary goal of ''The Grande Ronde Basin Fish Habitat Improvement Project'' is to access, create, improve, protect, and restore reparian and instream habitat for anadromous salmonids, thereby maximizing opportunities for natural fish production within the basin.

  1. Establishment of blue mussel beds to enhance fish habitats

    DEFF Research Database (Denmark)

    Kristensen, Louise Dahl; Stenberg, Claus; Støttrup, Josianne

    2015-01-01

    Human activity has impacted many coastal fjords causing degeneration of the structure and function of the fish habitats. In Nørrefjord, Denmark, local fishermen complained of declining fish catches which could be attributed to eutrophication and extraction of sediments over several decades...... directly on hemp sacs hanging on long-lines was the most effective method. This new method is potentially a useful management tool to improve fish habitats...

  2. Habitat Restoration/Enhancement Fort Hall Reservation : 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Moser, David C.

    2003-03-01

    Habitat enhancement, protection and monitoring were the focus of the Resident Fisheries Program during 2001. Enhancement and protection included sloping, fencing and planting willows at sites on Diggie Creek, Clear Creek and Spring Creek. In addition, many previously constructed instream structures (rock barbs and wing dams) were repaired throughout the Fort Hall Indian Reservation (Reservation). In 2001, exclosure fences were erected on Diggie Creek (250 m barbed wire; (70 m jack), Wood Creek (500 m jack), Clear Creek (20 m jack), Ross Fork Creek (200 m jack), West Fork Creek (200 m jack)) and the Portneuf River (1 km barbed wire; 100 m jack). Jack and rail exclosure fences that had deteriorated over the past ten years were repaired at numerous areas throughout the Reservation. Physical sampling during 2001 included sediment and depth surveys (SADMS) in Big Jimmy Creek and Diggie Creek. SADMS, used to track changes in channel morphology and specifically track movements of silt through Bottoms stream systems were completed for eight and nine strata in the Big Jimmy and Diggie Creek, respectively. Baseline SADM data was collected in Diggie Creek to monitor the effects of bank sloping and revegetation on channel morphology and sediment levels through time. Water temperature was monitored (hourly) in Spring Creek, Clear Creek, Ross Fork Creek and Big Jimmy Creek. Biotic sampling included invertebrate sampling in the 200 and 300 series of Clear Creek. Fish population densities and biomass were sampled in Clear Creek 200 and 300 series. Sampling protocols were identical to methods used in past years. Numbers of fish in Clear Creek 300 series remained similar to 2000 while numbers of fish in Clear Creek 200 series dropped to near pre project levels. Salmonid fry densities were monitored near Broncho Bridge and were significantly higher than 2000. A mark-recapture study was initiated in spring 2001 to estimate numbers of spawning adults using the Head End of Spring Creek

  3. FSH isoform pattern in classic galactosemia

    OpenAIRE

    Gubbels, C.S.; Thomas, C. M.; Wodzig, K.W.H.; Olthaar, A. J.; Jaeken, J.; Sweep, F.C.; Rubio-Gozalbo, M.E.

    2010-01-01

    Female classic galactosemia patients suffer from primary ovarian insufficiency (POI). The cause for this long-term complication is not fully understood. One of the proposed mechanisms is that hypoglycosylation of complex molecules, a known secondary phenomenon of galactosemia, leads to FSH dysfunction. An earlier study showed less acidic isoforms of FSH in serum samples of two classic galactosemia patients compared to controls, indicating hypoglycosylation. In this study, FSH isoform patterns...

  4. Proresorptive actions of FSH and bone loss.

    Science.gov (United States)

    Zaidi, Mone; Blair, Harry C; Iqbal, Jameel; Zhu, Ling Ling; Kumar, T Rajendra; Zallone, Alberta; Sun, Li

    2007-11-01

    We review studies that propose follicle-stimulating hormone (FSH) as a physiologic stimulator of osteoclastic bone resorption. We hypothesize that, in addition to low estrogen, a rising FSH contributes to the increased bone resorption and bone loss in hypergonadism. This is of particular relevance to the perimenopausal transition, wherein profound bone loss is accompanied by trabecular perforations in the face of high FSH and normal estrogen levels. Potential therapeutic implications include the development of antagonists to both circulating FSH and its osteoclastic receptor.

  5. Endangered Roseate Tern Breeding and Staging Habitat Management and Enhancement Progress Report FY13-FY14

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The goals of this project are to enhance and protect roseate tern breeding and staging habitat to increase productivity, and improve survival of fledglings and...

  6. Endangered Roseate Tern Breeding and Staging Habitat Management and Enhancement Progress Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The goals of this project are to enhance and protect roseate tern breeding and staging habitat to increase productivity, and improve survival of fledglings and...

  7. Biodiversity enhances ecosystem multifunctionality across trophic levels and habitats.

    Science.gov (United States)

    Lefcheck, Jonathan S; Byrnes, Jarrett E K; Isbell, Forest; Gamfeldt, Lars; Griffin, John N; Eisenhauer, Nico; Hensel, Marc J S; Hector, Andy; Cardinale, Bradley J; Duffy, J Emmett

    2015-04-24

    The importance of biodiversity for the integrated functioning of ecosystems remains unclear because most evidence comes from analyses of biodiversity's effect on individual functions. Here we show that the effects of biodiversity on ecosystem function become more important as more functions are considered. We present the first systematic investigation of biodiversity's effect on ecosystem multifunctionality across multiple taxa, trophic levels and habitats using a comprehensive database of 94 manipulations of species richness. We show that species-rich communities maintained multiple functions at higher levels than depauperate ones. These effects were stronger for herbivore biodiversity than for plant biodiversity, and were remarkably consistent across aquatic and terrestrial habitats. Despite observed tradeoffs, the overall effect of biodiversity on multifunctionality grew stronger as more functions were considered. These results indicate that prior research has underestimated the importance of biodiversity for ecosystem functioning by focusing on individual functions and taxonomic groups.

  8. South Fork Clearwater River Habitat Enhancement, Nez Perce National Forest.

    Energy Technology Data Exchange (ETDEWEB)

    Siddall, Phoebe

    1992-04-01

    In 1984, the Nez Perce National forest and the Bonneville Power Administration entered into a contractual agreement which provided for improvement of spring chinook salmon and summer steelhead trout habitat in south Fork Clearwater River tributaries. Project work was completed in seven main locations: Crooked River, Red River, Meadow Creek Haysfork Gloryhole, Cal-Idaho Gloryhole, Fisher Placer and Leggett Placer. This report describes restoration activities at each of these sites.

  9. Umatilla River Basin Anadromous Fish Habitat Enhancement Project : 1995 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Shaw, R.Todd

    1996-05-01

    During the 1995 - 96 project period, four new habitat enhancement projects were implemented under the Umatilla River Basin Anadromous Fish Habitat Enhancement Project by the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) in the upper Umatilla River Basin. A total of 38,644 feet of high tensile smooth wire fencing was constructed along 3.6 miles of riparian corridor in the Meacham Creek, Wildhorse Creek, Greasewood Creek, West Fork of Greasewood Creek and Mission Creek watersheds. Additional enhancements on Wildhorse Creek and the lower Greasewood Creek System included: (1) installation of 0.43 miles of smooth wire between river mile (RM) 10.25 and RM 10.5 Wildhorse Creek (fence posts and structures had been previously placed on this property during the 1994 - 95 project period), (2) construction of 46 sediment retention structures in stream channels and maintenance to 18 existing sediment retention structures between RM 9.5 and RM 10.25 Wildhorse Creek, and (3) revegetation of stream corridor areas and adjacent terraces with 500 pounds of native grass seed or close species equivalents and 5,000 native riparian shrub/tree species to assist in floodplain recovery, stream channel stability and filtering of sediments during high flow periods. U.S. Fish and Wildlife Service (USFWS), Bureau of Indian Affairs (BIA) and Environmental Protection Agency (EPA) funds were cost shared with Bonneville Power Administration (BPA) funds, provided under this project, to accomplish habitat enhancements. Water quality monitoring continued and was expanded for temperature and turbidity throughout the upper Umatilla River Watershed. Physical habitat surveys were conducted on the lower 13 river miles of Wildhorse Creek and within the Greasewood Creek Project Area to characterize habitat quality and to quantify various habitat types by area.

  10. FSH isoform pattern in classic galactosemia.

    Science.gov (United States)

    Gubbels, Cynthia S; Thomas, Chris M G; Wodzig, Will K W H; Olthaar, André J; Jaeken, Jaak; Sweep, Fred C G J; Rubio-Gozalbo, M Estela

    2011-04-01

    Female classic galactosemia patients suffer from primary ovarian insufficiency (POI). The cause for this long-term complication is not fully understood. One of the proposed mechanisms is that hypoglycosylation of complex molecules, a known secondary phenomenon of galactosemia, leads to FSH dysfunction. An earlier study showed less acidic isoforms of FSH in serum samples of two classic galactosemia patients compared to controls, indicating hypoglycosylation. In this study, FSH isoform patterns of five classic galactosemia patients with POI were compared to the pattern obtained in two patients with a primary glycosylation disorder (phosphomannomutase-2-deficient congenital disorders of glycosylation, PMM2-CDG) and POI, and in five postmenopausal women as controls. We used FPLC chromatofocussing with measurement of FSH concentration per fraction, and discovered that there were no significant differences between galactosemia patients, PMM2-CDG patients and postmenopausal controls. Our results do not support that FSH dysfunction due to a less acidic isoform pattern because of hypoglycosylation is a key mechanism of POI in this disease.

  11. Developing a top-down land-use management procedure for fish habitat enhancement

    Science.gov (United States)

    Chiang, Li-Chi; Lin, Yu-Pin; Wu, Chen-Huan

    2013-04-01

    Land-use change can influence stream ecosystem and alter instream physical, chemical and biological habitat. For example, urbanization usually contributes to increasing sediment loadings to streams and inappropriate agricultural management results in degradation of stream water quality. Watershed model is an effective way to forecast the watershed response to different land-use change scenarios. We developed a top-down approach from the watershed scale to the microscale by combining the habitat model, land-use change model and watershed hydrological model. This approach can assist land-use planner to make optimal decisions with fish habitat enhancement. The study was conducted in Datuan Stream, located in Tamsui District, New Taipei City and the target species is monk goby (Sicyopterus japonicus). The spatially explicit land-use change model, CLUE-s was first applied to project several future land-use scenarios and the Soil and Water Assessment Tool (SWAT) was then applied to simulate streamflow for different land-use scenarios. The simulated streamflow were used as input data for simulating river habitat, where Habitat Suitability Analysis is one of the most important processes. The relationship between target species and multiple environmental factors of habitat was first developed using the Habitat suitability index (HSI). In this study, we used fish presence probabilities for each velocity and water depth to establish different HSI functions under 4 flow conditions (slack, riffle, pool and run) using genetic programming (GP). The physical habitat model, River 2D, was then applied to simulate the river section and calculate weighted usable area (WUA). Based on the WUA results for different land-use scenarios, we further evaluated the relationships between WUA and land-use/landscape patterns using a spatial pattern analysis program, Fragstats. The results showed that by using the habitat model for classified flows, the habitat suitability curve which reflects

  12. Umatilla River Basin Anadromous Fish Habitat Enhancement Project : 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Shaw, R. Todd; Sexton, Amy D.

    2003-02-01

    The Umatilla River Basin Anadromous Fish Habitat Enhancement Project continued to identify impacted stream reaches throughout the Umatilla River Basin for habitat improvements during the 2001 project period. Public outreach efforts, biological and physical monitoring, and continued development of a Umatilla Subbasin Watershed Assessment assisted the project in fostering public cooperation, targeting habitat deficiencies and determining habitat recovery measures. Projects continued to be maintained on 49 private properties, one 25-year Non-Exclusive Bureau of Indian Affairs' Easement was secured, six new projects implemented and two existing project areas improved to enhance anadromous fish habitat. New project locations included sites on the mid Umatilla River, upper Umatilla River, Mission Creek, Cottonwood Creek and Buckaroo Creek. New enhancements included: (1) construction of 11,264 feet of fencing between River Mile 43.0 and 46.5 on the Umatilla River, (2) a stream bank stabilization project implemented at approximately River Mile 63.5 Umatilla River to stabilize 330 feet of eroding stream bank and improve instream habitat diversity, included construction of eight root wad revetments and three boulder J-vanes, (3) drilling a 358-foot well for off-stream livestock watering at approximately River Mile 46.0 Umatilla River, (4) installing a 50-foot bottomless arch replacement culvert at approximately River Mile 3.0 Mission Creek, (5) installing a Geoweb stream ford crossing on Mission Creek (6) installing a 22-foot bottomless arch culvert at approximately River Mile 0.5 Cottonwood Creek, and (7) providing fence materials for construction of 21,300 feet of livestock exclusion fencing in the Buckaroo Creek Drainage. An approximate total of 3,800 native willow cuttings and 350 pounds of native grass seed was planted at new upper Umatilla River, Mission Creek and Cottonwood Creek project sites. Habitat improvements implemented at existing project sites included

  13. Umatilla River Basin Anadromous Fish Habitat Enhancement Project : 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Shaw, R. Todd; Sexton, Amy D.

    2003-02-01

    The Umatilla River Basin Anadromous Fish Habitat Enhancement Project continued to identify impacted stream reaches throughout the Umatilla River Basin for habitat improvements during the 2001 project period. Public outreach efforts, biological and physical monitoring, and continued development of a Umatilla Subbasin Watershed Assessment assisted the project in fostering public cooperation, targeting habitat deficiencies and determining habitat recovery measures. Projects continued to be maintained on 49 private properties, one 25-year Non-Exclusive Bureau of Indian Affairs' Easement was secured, six new projects implemented and two existing project areas improved to enhance anadromous fish habitat. New project locations included sites on the mid Umatilla River, upper Umatilla River, Mission Creek, Cottonwood Creek and Buckaroo Creek. New enhancements included: (1) construction of 11,264 feet of fencing between River Mile 43.0 and 46.5 on the Umatilla River, (2) a stream bank stabilization project implemented at approximately River Mile 63.5 Umatilla River to stabilize 330 feet of eroding stream bank and improve instream habitat diversity, included construction of eight root wad revetments and three boulder J-vanes, (3) drilling a 358-foot well for off-stream livestock watering at approximately River Mile 46.0 Umatilla River, (4) installing a 50-foot bottomless arch replacement culvert at approximately River Mile 3.0 Mission Creek, (5) installing a Geoweb stream ford crossing on Mission Creek (6) installing a 22-foot bottomless arch culvert at approximately River Mile 0.5 Cottonwood Creek, and (7) providing fence materials for construction of 21,300 feet of livestock exclusion fencing in the Buckaroo Creek Drainage. An approximate total of 3,800 native willow cuttings and 350 pounds of native grass seed was planted at new upper Umatilla River, Mission Creek and Cottonwood Creek project sites. Habitat improvements implemented at existing project sites included

  14. The potential of fruit trees to enhance converted habitats for migrating birds in southern Mexico

    Science.gov (United States)

    Foster, M.S.

    2007-01-01

    Migration routes used by Nearctic migrant birds can cover great distances; they also differ among species, within species, and between years and seasons. As a result, migration routes for an entire migratory avifauna can encompass broad geographic areas, making it impossible to protect continuous stretches of habitat sufficient to connect the wintering and breeding grounds for most species. Consequently, ways to enhance habitats converted for human use (i.e. for pasture, crop cultivation, human settlement) as stopover sites for migrants are especially important. Shelterbelts around pastures and fields, if planted with species targeted to support migrant (and resident) bird species that naturally occupy mature forest habitats and that are at least partially frugivorous, could be a powerful enhancement tool for such species, if the birds will enter the converted areas to feed. I tested this approach for Nearctic migrant birds during the spring migration through an area in Chiapas, Mexico. Mature forest tree species whose fruits are eaten by birds were surveyed. Based on life form, crop size and fruit characteristics, I selected three tree species for study: Cymbopetalum mayanum (Annonaceae), Bursera simaruba (Burseraceae) and Trophis racemosa (Moraceae). I compared the use of fruits of these species by migrants and residents in forest with their use of the fruits of isolated individuals of the same species in pasture and cropland. All three plant species were useful for enhancing converted habitats for forest-occupying spring migrants, although species differed in the degree to which they entered disturbed areas to feed on the fruits. These tree species could probably enhance habitats for migrants at sites throughout the natural geographic ranges of the plants; in other geographic areas for other target bird groups, other tree species might be more appropriate.

  15. Abundance, Behavior, and Habitat Utilization by Coho Salmon and Steelhead Trout in Fish Creek, Oregon, as Influenced by Habitat Enhancement, 1985 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Wolfe, John (Mount Hood National Forest, Clackamas River Ranger District, Estacada, OR); Everest, Fred H. (Oregon State University, Pacific Northwest Forest and Range Experiment Station, Corvallis, OR); Heller, David A. (Mount Hood National Forest, Gresham, OR)

    1986-09-01

    Construction and evaluation of salmonid habitat improvements on Fish Creek, a tributary of the upper Clackamas River, was continued in fiscal year 1985 by the Estacada Ranger District, Mt. Hood National Forest, and the Anadromous Fish Habitat Research Unit of the Pacific Northwest Forest and Range Experiment Station (PNW), USDA Forest Service. The study began in 1982 when PNW entered into an agreement with the Mt. Hood National Forest to evaluate fish habitat improvements in the Fish Creek basin on the Estacada Ranger District. The project was initially conceived as a 5-year effort (19824986) to be financed by Forest Service funds. Several factors limiting production of salmonids in the basin were identified during the first year of the study, and the scope of the habitat improvement effort was subsequently enlarged. The habitat improvement program and the evaluation of improvements were both expanded in mid-1983 when the Bonneville Power Administration entered into an agreement with the Mt. Hood National Forest to provide additional funding for work on Fish Creek. Habitat improvement work in the basin is designed to increase the annual number of chinook and coho salmon, and steelhead trout smolt outmigrants. The primary objectives of the evaluation include the: (1) Evaluation and quantification of changes in salmonid spawning and rearing habitat resulting from a variety of habitat Improvements. (2) Evaluation and quantification of changes in fish populations and biomass resulting from habitat improvements. (3) Evaluation of the cost-effectiveness of habitat improvements developed with BPA and Forest Service funds on Fish Creek. Several prototype enhancement projects were constructed and tested during the first three years of the study. The Intention was to identify successful techniques that could then be broadly applied within the bash. This stepwise procedure has been largely successful in identifying the most promising enhancement techniques for the Fish Creek

  16. Grande Ronde Basin Fish Habitat Enhancement Project, Annual Report 2002-2003.

    Energy Technology Data Exchange (ETDEWEB)

    McGowan, Vance

    2003-08-01

    On July 1, 1984 the Bonneville Power Administration and the Oregon Department of Fish and Wildlife entered into an agreement to initiate fish habitat enhancement work in the Joseph Creek subbasin of the Grande Ronde River Basin in northeast Oregon. In July of 1985 the Upper and Middle Grande Ronde River, and Catherine Creek subbasins were included in the intergovernmental contract, and on March 1, 1996 the Wallowa River subbasin was added. The primary goal of 'The Grande Ronde Basin Fish Habitat Enhancement Project' is to create, protect, and restore riparian and instream habitat for anadromous salmonids, thereby maximizing opportunities for natural fish production within the basin. This project provided for implementation of Program Measure 703 (C)(1), Action Item 4.2 of the Northwest Power Planning Council's Columbia River Basin Fish and Wildlife Program (NPPC, 1987), and continues to be implemented as offsite mitigation for mainstem fishery losses caused by the Columbia River hydro-electric system. All work conducted by the Oregon Department of Fish and Wildlife is on private lands and therefore requires that considerable time be spent developing rapport with landowners to gain acceptance of, and continued cooperation with this program throughout 10-15 year lease periods. This project calls for passive regeneration of habitat, using riparian exclosure fencing as the primary method to restore degraded streams to a normative condition. Active remediation techniques using plantings, off-site water developments, site-specific instream structures, or whole channel alterations are also utilized where applicable. Individual projects contribute to and complement ecosystem and basin-wide watershed restoration efforts that are underway by state, federal, and tribal agencies, and local watershed councils. Work undertaken during 2002 included: (1) Implementing 1 new fencing project in the Wallowa subbasin that will protect an additional 0.95 miles of stream

  17. John Day River Subbasin Fish Habitat Enhancement Project, 1991 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Neal, Jeff A.; Jerome, James P.; Delano, Kenneth H.

    1993-05-01

    The purpose of the John Day Fish Habitat Enhancement Program is to enhance production of indigenous wild stocks of spring chinook and summer steelhead within the subbasin through habitat enhancement and access improvement. The John Day River system supports the largest remaining wild runs of spring chinook salmon and summer steelhead in northeast Oregon. It is the goal of this program to preserve and enhance the unique genetic component of the stocks. By attaining this goal we will be able to rebuild fish runs in other Columbia River tributaries in the future, if desired. During 1991, 5 leases were signed adding 5.25 miles of stream to the program. Fence construction included 9.95 miles of riparian fence and 15 livestock water crossings. We constructed 3 log wiers for adult salmon holding, added 280 ft. of new channel, and placed 274 fish habitat boulders, 6 trees and 31 rootwads for juvenile rearing. We constructed 15 stream deflectors and 274 linear feet of bank riprap for streambank stabilization.

  18. FSH isoform pattern in classic galactosemia

    NARCIS (Netherlands)

    Gubbels, C.S.; Thomas, C.M.G.; Wodzig, W.K.; Olthaar, A.J.; Jaeken, J.; Sweep, F.C.; Rubio-Gozalbo, M.E.

    2011-01-01

    Female classic galactosemia patients suffer from primary ovarian insufficiency (POI). The cause for this long-term complication is not fully understood. One of the proposed mechanisms is that hypoglycosylation of complex molecules, a known secondary phenomenon of galactosemia, leads to FSH dysfuncti

  19. Artificial breakwaters as garbage bins: Structural complexity enhances anthropogenic litter accumulation in marine intertidal habitats.

    Science.gov (United States)

    Aguilera, Moisés A; Broitman, Bernardo R; Thiel, Martin

    2016-07-01

    Coastal urban infrastructures are proliferating across the world, but knowledge about their emergent impacts is still limited. Here, we provide evidence that urban artificial reefs have a high potential to accumulate the diverse forms of litter originating from anthropogenic activities around cities. We test the hypothesis that the structural complexity of urban breakwaters, when compared with adjacent natural rocky intertidal habitats, is a driver of anthropogenic litter accumulation. We determined litter abundances at seven sites (cities) and estimated the structural complexity in both urban breakwaters and adjacent natural habitats from northern to central Chile, spanning a latitudinal gradient of ∼15° (18°S to 33°S). Anthropogenic litter density was significantly higher in coastal breakwaters when compared to natural habitats (∼15.1 items m(-2) on artificial reefs versus 7.4 items m(-2) in natural habitats) at all study sites, a pattern that was temporally persistent. Different litter categories were more abundant on the artificial reefs than in natural habitats, with local human population density and breakwater extension contributing to increase the probabilities of litter occurrence by ∼10%. In addition, structural complexity was about two-fold higher on artificial reefs, with anthropogenic litter density being highest at intermediate levels of structural complexity. Therefore, the spatial structure characteristic of artificial reefs seems to enhance anthropogenic litter accumulation, also leading to higher residence time and degradation potential. Our study highlights the interaction between coastal urban habitat modification by establishment of artificial reefs, and pollution. This emergent phenomenon is an important issue to be considered in future management plans and the engineering of coastal ecosystems.

  20. Umatilla River Basin Anadromus Fish Habitat Enhancement Project : 1994 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Shaw, R. Todd

    1994-05-01

    The Umatilla Basin Anadromous Fish Habitat Enhancement Project is funded under the Northwest Power Planning Council's Columbia River Basin Fish and Wildlife Program, Section 7.6-7.8 and targets the improvement of water quality and restoration of riparian areas, holding, spawning and rearing habitats of steelhead, spring and fall chinook and coho salmon. The project focused on implementing cooperative instream and riparian habitat improvements on private lands on the Umatilla Indian Reservation (hereafter referred to as Reservation) from April 1, 1988 to March 31, 1992. These efforts resulted in enhancement of the lower l/4 mile of Boston Canyon Creek, the lower 4 river miles of Meacham Creek and 3.2 river miles of the Umatilla River in the vicinity of Gibbon, Oregon. In 1993, the project shifted emphasis to a comprehensive watershed approach, consistent with other basin efforts, and began to identify upland and riparian watershed-wide causative factors impacting fisheries habitat and natural fisheries production capabilities throughout the Umatilla River Watershed. During the 1994-95 project period, a one river mile demonstration project was implemented on two privately owned properties on Wildhorse Creek. This was the first watershed improvement project to be implemented by the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) off of the Reservation. Four 15 year riparian easements and two right-of-way agreements were secured for enhancement of one river mile on Wildhorse Creek and l/2 river mile on Meacham Creek. Enhancements implemented between river mile (RM) 9.5 and RM 10.5 Wildhorse Creek included: (1) installation of 1.43 miles of smooth wire high tensile fence line and placement of 0.43 miles of fence posts and structures to restrict livestock from the riparian corridor, (2) construction of eighteen sediment retention structures in the stream channel to speed riparian recovery by elevating the stream grade, slowing water velocities and

  1. John Day River Sub-Basin Fish Habitat Enhancement Project; 2008 Annual Report

    Energy Technology Data Exchange (ETDEWEB)

    Powell, Russ M.; Alley, Pamela D.; Goin Jr, Lonnie [Oregon Department of Fish and Wildlife

    2009-07-15

    Work undertaken in 2008 included: (1) Seven new fence projects were completed thereby protecting approximately 10.97 miles of streams with 16.34 miles of riparian fence; (2) Renewal of one expired lease was completed thereby continuing to protect 0.75 miles of stream with 1.0 mile of riparian fence. (3) Maintenance of all active project fences (106.54 miles), watergaps (78), spring developments (33) were checked and repairs performed; (3) Planted 1000 willow/red osier on Fox Creek/Henslee property; (4) Planted 2000 willows/red osier on Middle Fork John Day River/Coleman property; (5) Planted 1000 willow/red osier cuttings on Fox Creek/Johns property; (6) Since the initiation of the Fish Habitat Project in 1984 we have 126.86 miles of stream protected using 211.72 miles of fence protecting 5658 acres. The purpose of the John Day Fish Habitat Enhancement Program is to enhance production of indigenous wild stocks of spring Chinook and summer steelhead within the sub basin through habitat protection, enhancement and fish passage improvement. The John Day River system supports the largest remaining wild runs of spring chinook salmon and summer steelhead in Northeast Oregon.

  2. Clackamas/Hood River Habitat Enhancement Project; Implementation Plan, 1988-1992 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Medel, Ron; Hohler, David B. (Mount Hood National Forest, Clackamas River Ranger District, Estacada, OR); MacDonald, Ken (Mount Hood National Forest, Hood River Ranger District, Parkdale, OR)

    1988-01-01

    An Implementation Plan and Statement of Work is provided for high priority work in the Clackamas. Hood River and Fifteenmile sub-basins. These documents describe fish habitat improvement opportunities that can be implemented by the 1991 deadline established by the Northwest Power Planning Council. The Clackamas/Hood River Enhancement Program is an on-going project initiated in 1984. It is being cooperatively funded by the Bonneville Power Administration and the Wt. Hood National Forest. Species for management emphasis include spring chinook and coho salmon, and summer and winter steelhead trout. Improvement activities are designed to improve access at passage barriers and increase the quality and quantity of available rearing habitat. Project work will result in improved access to about 12.5 miles of high quality habitat, creation of nearly 70,000 square feet of off-channel habitat, and the addition of structure to approximately 32 miles of stream. At completion of the project, annual production capability from these two sub-basins will be increased by 85-100,000 smolts. Details of a monitoring and evaluation effort consistent with measure 200(d)(l) of the Council's Fish and Wildlife Program are also provided.

  3. Duck Valley Habitat Enhancement and Protection, 2001-2002 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Allen, Mattie H.; Sellman, Jake (Shoshone-Paiute Nation, Duck Valley Indian Reservation, Owyhee, NV)

    2003-03-01

    The Duck Valley Indian Reservation's Habitat Enhancement project is an ongoing project designed to enhance and protect critical riparian areas, natural springs, the Owhyee River and its tributaries, and native fish spawning areas on the Reservation. The project commenced in 1997 and addresses the Northwest Power Planning Council's measures 10.8C.2, 10.8C.3, and 10.8C.5 of the 1994 Columbia River Basin Fish and Wildlife Program. The performance period covers dates from April 2001 through August 2002.

  4. Evaluation and Monitoring of Idaho Habitat Enhancement and Anadromous Fish Natural Production : Annual Report 1986.

    Energy Technology Data Exchange (ETDEWEB)

    Petrosky, Charles E.; Holubetz, Terry B.

    1987-11-01

    The Idaho Department of Fish and Game (IDFG) has been conducting an evaluation of existing and proposed habitat improvement projects for anadromous fish in the Clearwater River and Salmon River drainages over the last 3 years. Projects included in the evaluation are funded by or proposed for funding by the Bonneville Power Administration (BPA) under the Northwest Power Planning Act as off-site mitigation for downstream hydropower development on the Snake and Columbia rivers. This evaluation project is also funded under the same authority. A mitigation record is being developed to use increased smolt production (i.e., yield) at full-seeding as the best measure of benefit from a habitat enhancement project. Determination of full benefit from a project depends on completion or maturation of the project and presence of adequate numbers of fish to document actual increases in fish production. The depressed nature of upriver anadromous stocks have precluded measuring full benefits of any habitat enhancement project in Idaho. Partial benefit will be credited to the mitigation record in the interim period of run restoration.

  5. CDC25B Involved in Proliferation of Sertoli Cells of New Born Calves Through FSH and Possibly Being Key Regulating Factor

    Institute of Scientific and Technical Information of China (English)

    Tian Ya-guang

    2016-01-01

    The effects of FSH on the proliferation of sertoli cells of new born calves were studied in order to provide some data for theoretical research and practical use of spermatogenesis in vitro. Different concentrations of FSH (0, 0.01, 0.02, 0.04, and 0.08 IU• mL-1) were taken to treat bovine sertoli cellsin vitro culture, the number of sertoli cells and the expression of seven genes were determined at 6, 12 and 24 h after FSH treatments. FSH could significantly promote the proliferation ofin vitrocultured sertoli cells. FSH had no significant effects on the expression of CDC25A and could significantly improve the expression of CDC25B. 0.04 IU• mL-1 and 0.08 IU• mL-1 FSH treatments decreased the expression of CDC25C at 12 h. 0.08 IU• mL-1 FSH treatment decreased the expression of CDC25C at 24 h. 0.04 IU• mL-1 FSH could significantly decrease the expression of GSK-3β and improve the expression ofβ-catenin at 6, 12 and 24 h. 0.02, 0.04 and 0.08 IU• mL-1 FSH treatments enhanced the expressions of CYCLIND1 and C-MYC. In conclusion, FSH promoted the proliferation of sertoli cells and 0.04 IU• mL-1 FSH concentration could significantly promote the proliferation ofin vitrocultured sertoli cells. FSH promoted the proliferation of sertoli cells by CDC25B and WNT/β-catenin and CDC25B might be the key regulator to the proliferating rate of sertoli cells of bovine calf.

  6. Habitat-specific AMF symbioses enhance drought tolerance of a native Kenyan grass

    Science.gov (United States)

    Petipas, Renee H.; González, Jonathan B.; Palmer, Todd M.; Brody, Alison K.

    2017-01-01

    The role of arbuscular mycorrhizal fungi (AMF) in enhancing plant tolerance to drought is well known. However, the degree to which AMF-plant symbioses are locally adapted has been suggested but is less well understood, especially at small spatial scales. Here, we examined the effects of two arbuscular mycorrhizal fungal communities on drought tolerance of Themeda triandra, a native African perennial bunchgrass. In our study area, mound building activities of Odontotermes sp. termites produce heterogeneous habitat, particularly with respect to water availability, and do so over small spatial scales (fungi from on or off termite mounds, or with a sterilized control inoculum. Our results reveal habitat-specific AMF effects on host stomatal functioning and growth. Contrary to our expectations, drought stressed grasses inoculated with AMF from termite mounds closed stomata less, and produced 60% more leaves than those inoculated with off-mound AMF, thus exhibiting higher levels of tolerance. Mound-inoculated plants that were drought stressed also produced more than twice as many leaves as non-inoculated plants. Longer-term productivity measurements indicate both on- and off-mound inoculated plants were able to recover to a greater extent than non-inoculated plants, indicating that AMF associations in general help plants recover from drought. These findings highlight the important role that AMF play in mitigating drought stress and indicate that AMF affect how plants experience drought in a small scale, habitat-specific manner.

  7. Grazer removal and nutrient enrichment as recovery enhancers for overexploited rocky subtidal habitats.

    Science.gov (United States)

    Guarnieri, Giuseppe; Bevilacqua, Stanislao; Vignes, Fabio; Fraschetti, Simonetta

    2014-07-01

    Increasing anthropogenic pressures are causing long-lasting regime shifts from high-diversity ecosystems to low-diversity degraded ones. Understanding the effects of multiple threats on ecosystems, and identifying processes allowing for the recovery of biodiversity, are the current major challenges in ecology. In several temperate marine areas, large parts of rocky subtidal habitats characterised by high diversity have been completely degraded to barren grounds by overfishing, including illegal date mussel fishing. Bare areas are characterized by the dominance of sea urchins whose grazing perpetuates the impact of overfishing. We investigated experimentally the separate and combined effects of nutrient enrichment and sea urchin exclusion on the recovery of barren grounds. Our results indicate that the two factors have a synergistic effect leading to the re-establishment of erect macroalgal canopies, enhancing the structural complexity of subtidal assemblages. In particular, in the overfished system considered here, the recovery of disturbed assemblages could occur only if sea urchins are removed. However, the recolonization of barren grounds by erect macroalgae is further enhanced under enriched conditions. This study demonstrates that the recovery of dramatically depleted marine habitats is possible, and provides useful indications for specific management actions, which at present are totally lacking, to achieve the restoration of barren grounds caused by human activity.

  8. John Day River Subbasin Fish Habitat Enhancement Project, 2002 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Powell, Russ M.; Jerome, James P.; Delano, Kenneth H.

    2003-03-01

    Work undertaken in 2002 included: (1) Seven new fence projects were completed thereby protecting 6.0 miles of stream (2) Completion of 0.7 miles of dredge tail leveling on Granite Creek. (3) New fence construction (300ft) plus one watergap on Indian Creek/ Kuhl property. (4) Maintenance of all active project fences (58.76 miles), watergaps (56), spring developments (32) and plantings were checked and repairs performed. (5) Restoration and Enhancement projects protected 3 miles of stream within the basin. (6) Since the initiation of the Fish Habitat Project in 1984 we have 67.21 miles of stream protected using 124.2 miles of fence. With the addition of the Restoration and Enhancement Projects we have 199.06 miles of fence protecting 124.57 miles of stream.

  9. NO-mediated regulation of GLUT by T3 and FSH in rat granulosa cells.

    Science.gov (United States)

    Tian, Ye; Ding, Yu; Liu, Juan; Heng, Dai; Xu, Kaili; Liu, Wenbo; Zhang, Cheng

    2017-03-17

    Thyroid hormones (THs) are important for normal reproductive function. Although 3,5,3'-triiodothyronine (T3) enhances follicle-stimulating hormone (FSH)-induced preantral follicle growth and granulosa cells development in vitro, little is known about the molecular mechanisms regulating ovarian development via glucose. In this study, we investigated whether and how T3 combines with FSH to regulate glucose transporter protein (GLUT) expression and glucose uptake in granulosa cells. Here, we present evidence that T3 and FSH co-treatment significantly increased GLUT-1/GLUT-4 expression, and translocation in cells, as well as glucose uptake. These changes were accompanied by upregulation of NOS3 expression, total NOS and NOS3 activity and NO content in granulosa cells. Furthermore, we found that activation of the mTOR and PI3K/Akt pathway is required for the regulation of GLUT expression, translocation, and glucose uptake by hormones. We also found that L-arginine (L-arg) up-regulated GLUT-1/GLUT-4 expression and translocation, which were related to increased glucose uptake, however, these responses were significantly blocked by L-NAME. In addition, inhibiting NO production attenuated T3 and FSH-induced GLUT expression, translocation, and glucose uptake in granulosa cells. Our data demonstrate that T3 and FSH co-treatment potentiates cellular glucose uptake via GLUT upregulation and translocation, which are mediated through the activation of the mTOR/PI3K/Akt pathway. Meanwhile, NOS3/NO are also involved in this regulatory system. These findings suggest that GLUT is a novel mediator of T3 and FSH-induced follicular development.

  10. A Framework for Enhancing Bird Habitat Value of Urban Greenspaces in the Woonasquatucket Watershed, Rhode Island, USA

    Science.gov (United States)

    Modifying greenspaces to enhance habitat value has been proposed as a means towards protecting or restoring biodiversity in urban landscapes. In this report, we provide a framework for developing low-cost, low-impact enhancements that can be incorporated during the restoration of...

  11. A Framework for Enhancing Bird Habitat Value of Urban Greenspaces in the Woonasquatucket Watershed, Rhode Island, USA

    Science.gov (United States)

    Modifying greenspaces to enhance habitat value has been proposed as a means towards protecting or restoring biodiversity in urban landscapes. In this report, we provide a framework for developing low-cost, low-impact enhancements that can be incorporated during the restoration of...

  12. Life stage and species identity affect whether habitat subsidies enhance or simply redistribute consumer biomass.

    Science.gov (United States)

    Keller, Danielle A; Gittman, Rachel K; Bouchillon, Rachel K; Fodrie, F Joel

    2017-08-18

    1.Quantifying the response of mobile consumers to changes in habitat availability is essential for determining the degree to which population-level productivity is habitat limited rather than regulated by other, potentially density-independent factors. 2.Over landscape scales, this can be explored by monitoring changes in density and foraging as habitat availability varies. As habitat availability increases, densities may: (1) decrease (unit-area production decreases; weak habitat limitation); (2) remain stable (unit-area production remains stable; habitat limitation); or (3) increase (unit-area production increases; strong habitat limitation). 3.We tested the response of mobile estuarine consumers over five months to changes in habitat availability in situ by comparing densities and feeding rates on artificial reefs that were or were not adjacent to neighboring artificial reefs or nearby natural reefs). 4.Using either constructed or natural reefs to manipulate habitat availability, we documented three-fold density decreases among juvenile stone crabs as habitat increased (i.e. weak habitat imitation). However, for adult stone crabs, density remained stable across treatments, demonstrating that habitat limitation presents a bottleneck in this species' later life history. Oyster toadfish densities also did not change with increasing habitat availability (i.e. habitat limitation), but densities of other cryptic fishes decreased as habitat availability increased (i.e. weak limitation). Feeding and abundance data suggested that some mobile fishes experience habitat limitation, or, potentially in one case, strong limitation across our habitat manipulations. 5.These findings of significant, community-level habitat limitation provide insight into how global declines in structurally complex estuarine habitats may have reduced the fishery production of coastal ecosystems. This article is protected by copyright. All rights reserved. This article is protected by copyright

  13. John Day River Subbasin Fish Habitat Enhancement Project, 2005-2006 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Powell, Russ M.; Alley, Pamela D.; Delano, Kenneth H. (Oregon Department of Fish and Wildlife, John Day, OR)

    2006-03-01

    Work undertaken in 2005 included: (1) Four new fence projects were completed thereby protecting 7.55 miles of stream with 9.1 miles of new riparian fence (2) Fence removal 1.7 miles of barbed wire. (3) Completed three spring developments (repair work on two BLM springs on Cottonwood Creek (Dayville), 1 solar on Rock Creek/ Collins property). (4) Dredge tail leveling completed on 0.9 miles of the Middle Fork of the John Day River (5) Cut, hauled and placed 30 junipers on Indian Creek/Kuhl property for bank stability. (6) Collected and planted 1500 willow cuttings on Mountain Creek/Jones property. (7) Conducted steelhead redd counts on Lake Cr./Hoover property and Cottonwood Cr./Mascall properties (8) Seeded 200 lbs of native grass seed on projects where the sites were disturbed by fence construction activities. (9) Maintenance of all active project fences (72.74 miles), watergaps (60), spring developments (30) were checked and repairs performed. (10) Since the initiation of the Fish Habitat Program in 1984 we have installed 156.06 miles of riparian fence on leased property protecting 88.34 miles of anadromous fish bearing stream. With the addition of the Restoration and Enhancement Projects from 1996-2001, where the landowner received the materials, built and maintained the project we have a total of 230.92 miles of fence protecting 144.7 miles of stream and 3285 acres of riparian habitat.

  14. John Day River Subbasin Fish Habitat Enhancement Project, 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Powell, Russ M.; Delano, Kenneth H.; Jerome, James P.

    2002-07-01

    Work undertaken in 2001 included: (1) 3335 structure posts were pounded on six new projects thereby protecting 10 miles of stream (2) Completion of 1000 ft. of barbed wire fence and one watergap on the Middle Fork of the John Day River/ Forrest property. (3) Fence removal of 5010 ft. of barbed wire fence on the Meredith project. (4) Maintenance of all active project fences (66 miles), watergaps (76), spring developments (32) and plantings were checked and repairs performed. (5) Since the initiation of the Fish Habitat Project in 1984 we have 63.74 miles of stream protected using 106.78 miles of fence. With the addition of the Restoration and Enhancement Projects we have 180.64 miles of fence protecting 120.6 miles of stream.

  15. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae in Cotton

    Directory of Open Access Journals (Sweden)

    P. G. Tillman

    2012-01-01

    Full Text Available Lady beetles (Coleoptera: Coccinellidae prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L., Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas, Coleomegilla maculata (De Geer, Cycloneda munda (Say, and Olla v-nigrum (Mulsant were found in sorghum over both years. Lady beetle compositions in sorghum and cotton and in yellow pyramidal traps were similar. For both years, density of lady beetles generally was higher on cotton with sorghum than on control cotton. Our results indicate that sorghum was a source of lady beetles in cotton, and thus incorporation of a sorghum habitat in farmscapes with cotton has great potential to enhance biocontrol of insect pests in cotton.

  16. Occurrence of postmenopausal-like acidic follicle-stimulating hormone (FSH) isoforms precedes the rise of FSH before menopause.

    NARCIS (Netherlands)

    Thomas, C.M.G.; Span, P.N.; Smeenk, J.M.J.; Hanssen, R.G.; Braat, D.D.M.; Sweep, F.C.

    2009-01-01

    OBJECTIVE: To assess the glycoform distribution patterns of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) during the menstrual cycle at different ages and FSH levels, after menopause, and with premature ovarian failure (POF). DESIGN: Controlled clinical study. SETTING: Healthy

  17. Learning the Hard Way: Imprinting Can Enhance Enforced Shifts in Habitat Choice

    Directory of Open Access Journals (Sweden)

    Niclas Vallin

    2011-01-01

    Full Text Available We investigated the potential importance of learning in habitat choice within a young hybrid zone of two closely related species of birds. Pied flycatchers (Ficedula hypoleuca are being excluded from deciduous habitats into a mixed forest type by collared flycatchers (F. albicollis. We investigated whether this enforced habitat shift influenced reproductive isolation between the two species, and, by cross-fostering nestlings, we tested whether learning may lead to a corresponding shift in habitat choice in consecutive generations. Our results show that the majority of the recruits, even if translocated across different habitat types, return to breed in the area where they were fostered. As male pied flycatchers were more likely to hybridize in the originally preferred habitat, we argue that early imprinting on an alternate habitat can play an important role in increasing reproductive isolation and facilitate regional coexistence between species experiencing secondary contact.

  18. Efficacy of Highly Purified Urinary FSH versus Recombinant FSH in Chinese Women over 37 Years Undergoing Assisted Reproductive Techniques

    Directory of Open Access Journals (Sweden)

    Xuemei Liu

    2015-02-01

    Full Text Available Background: Urine derived follicle-stimulating hormone (uFSH contains a higher proportion of acidic isoforms, whereas recombinant FSH (rFSH contains a higher proportion of less-acidic isoforms. Less-acidic isoforms have a faster clearance, and thus a shorter half-life than the acidic FSH isoforms. The slow clearance of the acidic isoforms has a longer half-life and higher biological activity. This study was designed to determine whether uFSH or rFSH is more effective in older Chinese women undergoing assisted reproductive techniques (ART. Materials and Methods: This is a prospective, randomized, controlled cohort study. A total of 508 Chinese women over 37 years were randomized into two following study groups for their in vitro fertilization (IVF or intracytoplasmic sperm injection (ICSI cycles: i. group A (n=254 were treated with rFSH, and ii. group B (n=254 were treated with uFSH. Both groups were suppressed with a gonadotropin-releasing hormone (GnRH analogue using a long down-regulation protocol. The main outcomes for comparison were days of stimulation, estradiol (E2 on the day of human chorionic gonadotropin (hCG administration, number of oocytes collected, amount of FSH used, quantity of FSH/oocyte, endometrial thickness at hCG day, M П oocyte rate, 2PN zygote rate, grade І embryo rate, number of embryos cryopreserved, pregnancy rate, implantation rate, abortion rate and the rate of no transferable embryos. Results: Twenty two cycles including 16 cycles with poor ovarian response and six cycles with ovarian hyperstimulation syndrome were cancelled. There were 243 cycles left in each group. The patients treated with uFSH had a significantly higher 2PN zygote rate (87.4 vs. 76.6%, p0.05. Conclusion: This study showed that uFSH produced a significantly higher proportion of grade І embryos than rFSH in older Chinese women and there was a significantly lower chance of no transferable embryos in uFSH cycles. The clinical efficacy of the two

  19. Inherited fungal symbionts enhance establishment of an invasive annual grass across successional habitats.

    Science.gov (United States)

    Uchitel, Andrea; Omacini, Marina; Chaneton, Enrique J

    2011-02-01

    Plants infected with vertically transmitted fungal endophytes carry their microbial symbionts with them during dispersal into new areas. Yet, whether seed-borne endophytes enhance the host plant's ability to overcome colonisation barriers and to regenerate within invaded sites remains poorly understood. We examined how symbiosis with asexual endophytic fungi (Neotyphodium) affected establishment and seed loss to predators in the invasive annual grass Lolium multiflorum (Italian ryegrass) across contrasting successional plots. Italian ryegrass seeds with high and low endophyte incidence were sown into three communities: a 1-year-old fallow field, a 15-year-old grassland, and a 24-year-old forest, which conformed to an old-field chronosequence in the eastern Inland Pampa, Argentina. We found that endophyte infection consistently increased host population recruitment and reproductive output. Endophyte presence also enhanced aerial biomass production of ryegrass in a low recruitment year but not in a high recruitment year, suggesting that symbiotic effects on growth performance are density dependent. Endophyte presence reduced seed removal by rodents, although differential predation may not account for the increased success of infected grass populations. Overall, there was no statistical evidence for an endophyte-by-site interaction, indicating that the fungal endosymbiont benefitted host establishment regardless of large differences in biotic and abiotic environment among communities. Our results imply that hereditary endophytes may increase the chances for host grass species to pass various ecological filters associated with invasion resistance across a broad range of successional habitats.

  20. Influence of forest and rangeland management on anadromous fish habitat in Western North America: rehabilitating and enhancing stream habitat—2. Field applications.

    Science.gov (United States)

    Gordon H. Reeves; Terry D. Roelofs

    1982-01-01

    Current techniques for rehabilitating and enhancing habitat to increase natural production of anadromous salmonids in the Pacific Northwest and Alaska are described. Methods to enhance spawning, rearing, and riparian habitat, and to improve access are reviewed. The information was compiled from published literature, unpublished reports by State and Federal agencies,...

  1. Follicle-stimulating hormone (FSH activates extracellular signal-regulated kinase phosphorylation independently of beta-arrestin- and dynamin-mediated FSH receptor internalization

    Directory of Open Access Journals (Sweden)

    Crepieux Pascale

    2006-06-01

    Full Text Available Abstract Background The follicle-stimulating hormone receptor (FSH-R is a seven transmembrane spanning receptor (7TMR which plays a crucial role in male and female reproduction. Upon FSH stimulation, the FSH-R activates the extracellular signal-regulated kinases (ERK. However, the mechanisms whereby the agonist-stimulated FSH-R activates ERK are poorly understood. In order to activate ERK, some 7 TMRs require beta-arrestin-and dynamin-dependent internalization to occur, whereas some others do not. In the present study, we examined the ability of the FSH-activated FSH-R to induce ERK phosphorylation, in conditions where its beta-arrestin- and dynamin-mediated internalization was impaired. Methods Human embryonic kidney (HEK 293 cells were transiently transfected with the rat FSH-R. Internalization of the FSH-R was manipulated by co-expression of either a beta-arrestin (319–418 dominant negative peptide, either an inactive dynamin K44A mutant or of wild-type beta-arrestin 1 or 2. The outcomes on the FSH-R internalization were assayed by measuring 125I-FSH binding at the cell surface when compared to internalized 125I-FSH binding. The resulting ERK phosphorylation level was visualized by Western blot analysis. Results In HEK 293 cells, FSH stimulated ERK phosphorylation in a dose-dependent manner. Co-transfection of the beta- arrestin (319–418 construct, or of the dynamin K44A mutant reduced FSH-R internalization in response to FSH, without affecting ERK phosphorylation. Likewise, overexpression of wild-type beta-arrestin 1 or 2 significantly increased the FSH-R internalization level in response to FSH, without altering FSH-induced ERK phosphorylation. Conclusion From these results, we conclude that the FSH-R does not require beta-arrestin- nor dynamin-mediated internalization to initiate ERK phosphorylation in response to FSH.

  2. Wanaket Wildlife Area Management Plan : Five-Year Plan for Protecting, Enhancing, and Mitigating Wildlife Habitat Losses for the McNary Hydroelectric Facility.

    Energy Technology Data Exchange (ETDEWEB)

    Confederated Tribes of the Umatilla Indian Reservation Wildlife Program

    2001-09-01

    The Confederated Tribes of the Umatilla Indian Reservation (CTUIR) propose to continue to protect, enhance, and mitigate wildlife and wildlife habitat at the Wanaket Wildlife Area. The Wanaket Wildlife Area was approved as a Columbia River Basin Wildlife Mitigation Project by the Bonneville Power Administration (BPA) and Northwest Power Planning Council (NWPPC) in 1993. This management plan will provide an update of the original management plan approved by BPA in 1995. Wanaket will contribute towards meeting BPA's obligation to compensate for wildlife habitat losses resulting from the construction of the McNary Hydroelectric facility on the Columbia River. By funding the enhancement and operation and maintenance of the Wanaket Wildlife Area, BPA will receive credit towards their mitigation debt. The purpose of the Wanaket Wildlife Area management plan update is to provide programmatic and site-specific standards and guidelines on how the Wanaket Wildlife Area will be managed over the next five years. This plan provides overall guidance on both short and long term activities that will move the area towards the goals, objectives, and desired future conditions for the planning area. The plan will incorporate managed and protected wildlife and wildlife habitat, including operations and maintenance, enhancements, and access and travel management. Specific project objectives are related to protection and enhancement of wildlife habitats and are expressed in terms of habitat units (HU's). Habitat units were developed by the US Fish and Wildlife Service's Habitat Evaluation Procedures (HEP), and are designed to track habitat gains and/or losses associated with mitigation and/or development projects. Habitat Units for a given species are a product of habitat quantity (expressed in acres) and habitat quality estimates. Habitat quality estimates are developed using Habitat Suitability Indices (HSI). These indices are based on quantifiable habitat features such

  3. Regeneration in bottomland forest canopy gaps six years after variable retention harvests to enhance wildlife habitat

    Science.gov (United States)

    Twedt, Daniel J.; Somershoe, Scott G.; Guldin, James M.

    2013-01-01

    To promote desired forest conditions that enhance wildlife habitat in bottomland forests, managers prescribed and implemented variable-retention harvest, a.k.a. wildlife forestry, in four stands on Tensas River National Wildlife Refuge, LA. These treatments created canopy openings (gaps) within which managers sought to regenerate shade-intolerant trees. Six years after prescribed harvests, we assessed regeneration in 41 canopy gaps and 4 large (>0.5-ha) patch cut openings that resulted from treatments and in 21 natural canopy gaps on 2 unharvested control stands. Mean gap area of anthropogenic gaps (582 m²) was greater than that of natural gaps (262 m²). Sweetgum (Liquidambar styraciflua) and red oaks (Quercus nigra, Q. nuttallii, and Q. phellos) were common in anthropogenic gaps, whereas elms (Ulmus spp.) and sugarberry (Celtis laevigata) were numerous in natural gaps. We recommend harvest prescriptions include gaps with diameter >25 m, because the proportion of shade-intolerant regeneration increased with gap area up to 500 m². The proportion of shade-intolerant definitive gap fillers (individuals likely to occupy the canopy) increased with gap area: 35 percent in natural gaps, 54 percent in anthropogenic gaps, and 84 percent in patch cuts. Sweetgum, green ash (Fraxinus pennsylvanica), and red oaks were common definitive gap fillers.

  4. Dose of recombinant FSH and oestradiol concentration on day of HCG affect embryo development kinetics

    DEFF Research Database (Denmark)

    Muñoz, Manuel; Cruz, María; Humaidan, Peter

    2012-01-01

    During follicular growth, the follicle is exposed to an almost ever-changing composition of isoforms of FSH and LH, which causes a number of different and divergent biological effects. Through a time-lapse system, embryo kinetics were examined following the use of FSH only (recombinant FSH, rFSH)...

  5. Supplement Analysis for the Watershed Management Program EIS - Idaho Model Watershed Habitat Projects - Welp Riparian Enhancement Fence

    Energy Technology Data Exchange (ETDEWEB)

    N/A

    2004-08-04

    The Bonneville Power Administration is proposing to fund the installation of approximately 1.5 miles of post and wire fence along Valley Creek in Stanley, Idaho. The proposed fence will meet or exceed BPA's minimum requirement of a 35-foot setback from the stream. Fence posts will be driven into the ground with a post ponder. The goal of this project is to enhance salmon and steelhead rearing and migration habitat through exclusion fencing.

  6. Follicle-stimulating Hormone (FSH) Induced Internalization of Porcine FSH Receptor in Cultured Porcine Granulosa Cells and Chinese Hamster Ovary Cells Transfected with Recombinant Porcine FSH Receptor cDNA

    Institute of Scientific and Technical Information of China (English)

    ZHU; Changhong; TIAN; Hong; XIONG; Zhongming; XIA; Huizhu

    2001-01-01

    In order to study the fate of human follicle-stimulating hormone (FSH) when hormone binds to its receptor, a quick biochemical method that can differentiate between the surface-bound and internalized hormone was used to determine the internalization induced by FSH in cultured both porcine granulosa cells and Chinese hamster ovary (CHO) cells expressing recombinant porcine FSH receptor. The results showed that FSH was slowly internalized, and the internalized radioactivity (acid resistant) reached a peak 10-12 h after addition of 125I-hFSH. It was suggested that FSHR do not get internalized rapidly under physiological circumstances precisely because the appropriate sequences are absent.

  7. Anti-FSH antibodies associate with poor outcome of ovarian stimulation in IVF.

    Science.gov (United States)

    Haller, Kadri; Salumets, Andres; Uibo, Raivo

    2008-03-01

    FSH is required for spontaneous folliculogenesis and is widely used in ovarian stimulation in IVF. Previously, increased concentrations of antibodies against FSH (anti-FSH) have been demonstrated in infertile women. This study aimed to: (i) assess the possible association of anti-FSH with an adverse outcome of IVF with regard to clinical parameters characterizing the ovarian reserve; and (ii) compare serum and follicular fluid (FF) anti-FSH concentrations in relation to follicle size and endocrine markers. IVF patients (n = 182) subjected to gonadotrophin-releasing hormone-antagonist protocol were assessed for anti-FSH using enzyme-linked immunosorbent assay. Increased concentrations of serum anti-FSH immunoglobulin (Ig)G and IgA were associated with impaired ovarian stimulation outcome, with cut-off values ratio for IgG = 6.95, P = 0.005 and IgA = 3.60, P = 0.039). FF anti-FSH IgG and IgA were positively associated with serum anti-FSH concentrations and FSH concentration in FF. Additionally, FF anti-FSH IgG increased with follicle growth (linear regression coefficient = 0.02, P = 0.022). Collectively, these data suggest that serum anti-FSH antibodies are associated with poor ovarian response to FSH stimulation in IVF, with anti-FSH IgA and IgG potentially exerting a local FSH antagonizing effect in maturing follicles.

  8. The effect of follicular fluid hormones on oocyte recovery after ovarian stimulation: FSH level predicts oocyte recovery

    Directory of Open Access Journals (Sweden)

    Rinaudo Paolo F

    2009-04-01

    Full Text Available Abstract Background Ovarian stimulation for assisted reproductive technology (ART overcomes the physiologic process to develop a single dominant follicle. However, following stimulation, egg recovery rates are not 100%. The objective of this study is to determine if the follicular fluid hormonal environment is associated with oocyte recovery. Methods This is a prospective study involving patients undergoing ART by standard ovarian stimulation protocols at an urban academic medical center. A total of 143 follicular fluid aspirates were collected from 80 patients. Concentrations of FSH, hCG, estradiol, progesterone, testosterone and prolactin were determined. A multivariable regression analysis was used to investigate the relationship between the follicular fluid hormones and oocyte recovery. Results Intrafollicular FSH was significantly associated with oocyte recovery after adjustment for hCG (Adjusted odds ratio (AOR = 1.21, 95%CI 1.03–1.42. The hCG concentration alone, in the range tested, did not impact the odds of oocyte recovery (AOR = 0.99, 95%CI 0.93–1.07. Estradiol was significantly associated with oocyte recovery (AOR = 0.98, 95% CI 0.96–0.99. After adjustment for progesterone, the strength of association between FSH and oocyte recovery increased (AOR = 1.84, 95%CI 1.45–2.34. Conclusion The relationship between FSH and oocyte recovery is significant and appears to work through mechanisms independent of the sex hormones. FSH may be important for the physiologic event of separation of the cumulus-oocyte complex from the follicle wall, thereby influencing oocyte recovery. Current methods for inducing the final stages of oocyte maturation, with hCG administration alone, may not be optimal. Modifications of treatment protocols utilizing additional FSH may enhance oocyte recovery.

  9. Heterogeneity of rat FSH by chromatofocusing: studies on serum FSH, hormone released in vitro and metabolic clearance rates of its various forms.

    Science.gov (United States)

    Blum, W F; Gupta, D

    1985-04-01

    Rat pituitary FSH was fractionated by chromatofocusing between pH 6 and 3. Ten components were resolved having apparent isoelectric points between 3.1 and 5.1. A comparative study of pituitary FSH and FSH secreted in vitro by quartered pituitary glands in the presence and in the absence of gonadotrophin-releasing hormone (GnRH) revealed similar patterns of charged species of intracellular and released FSH. Although GnRH increased FSH secretion about fourfold, no influence on the pattern of charged species was observed. Utilizing exclusion chromatography and chromatofocusing, pituitary FSH was compared to serum FSH which had been extracted by immuno-affinity chromatography. The results demonstrate for serum FSH a larger molecular size and a relative shift to more acidic components. Metabolic clearance rates of eight FSH components separated by chromatofocusing were measured in adult male rats. Half-lives varied between 13 min and several hours. A correlation existed between decrease of isoelectric points and decrease of metabolic clearance rates. These findings suggest that all hypophysial FSH components are secreted into the circulation at similar rates and the more acidic FSH components which appear to contain increased sialic acid, have a longer circulatory half-life and are more abundant in serum. It is concluded that sialylation may be involved in modulating serum FSH levels.

  10. Walla Walla River Basin Fish Habitat Enhancement Project, 2000-2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Volkman, Jed; Sexton, Amy D. (Confederated Tribes of the Umatilla Indian Reservation, Pendleton, OR)

    2001-01-01

    In 2000, the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) Fisheries Habitat Program implemented stream habitat restoration and protection efforts in the Walla Walla River Basin with funding from Bonneville Power Administration (BPA). The objective of these efforts is to protect and restore habitat critical to the recovery of weak or reintroduced populations of salmonid fish. Six projects, two on Couse Creek, two adjacent properties on Blue Creek, one on Patit Creek, and one property on the mainstem Walla Walla River were part of the exercise. Several thousand native plants as bare-root stock and cuttings were reintroduced to the sites and 18 acres of floodplain corridor was seeded with native grass seed. Pre and post-project monitoring efforts were included for all projects, incorporating methodologies from CTUIR's Draft Monitoring Plan.

  11. The Research Progress of FSH%FSH研究进展

    Institute of Scientific and Technical Information of China (English)

    华荣茂; 曾斌

    2014-01-01

    Follicle stimulating hormone (FSH) is glycoprotein hormone produced and secreted by animal anterior pituitary basophils. FSH has been widely used in the fields of animal husbandry and medicine. FSH is commonly applied in superovulation and in vitro fertilization (IVF) in the field of animal husbandry, and FSH beta genes closely related to animal breed. In the field of medicine, FSH is usually used in the treatment of diseases such as male and female infertility treatment, and it is closely related to menopausal women's bone mineral density (BMD) loss and women ovarian cancer.This paper briefly discusses the biological function and mechanism of FSH and summarized the research and application in the field of animal husbandry and medical field.%卵泡刺激素(Follicle stimulating hormone,FSH)是由动物脑垂体前叶嗜碱性细胞合成与分泌的一种糖蛋白类促性腺激素,其在畜牧业以及医学等领域有着广阔的应用。 FSH在畜牧业当中常应用于动物超数排卵、体外受精,并且FSHβ基因与动物产仔关系密切。在医学领域,FSH常用来治疗男女不孕不育等疾病,并且FSH与绝经期妇女骨密度(BMD)丢失以及女性卵巢癌等疾病也有很大的联系。文章简要概述FSH的生物学功能和机理,综述了其在畜牧业以及医学领域的研究与应用。

  12. Walla Walla River Basin Fish Habitat Enhancement Project, 2002-2003 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Volkman, Jed (Confederated Tribes of the Umatilla Indian Reservation, Pendleton, OR)

    2005-12-01

    In 2002 and 2003, the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) Fisheries Habitat Program implemented stream habitat restoration and protection efforts on private properties in the Walla Walla River Basin with funding from Bonneville Power Administration (BPA). The objective of this effort is to protect and restore habitat critical to the recovery of weak or reintroduced populations of salmonid fish. The CTUIR has currently enrolled nine properties into this program: two on Couse Creek, two adjacent properties on Blue Creek, one on Patit Creek, and four properties on the mainstem Walla Walla River. Major accomplishments during the reporting period include the following: (1) Secured approximately $229,000 in project cost share; (2) Purchase of 46 acres on the mainstem Walla Walla River to be protected perpetually for native fish and wildlife; (3) Developed three new 15 year conservation easements with private landowners; (4) Installed 3000 feet of weed barrier tarp with new plantings within project area on the mainstem Walla Walla River; (5) Expanded easement area on Couse Creek to include an additional 0.5 miles of stream corridor and 32 acres of upland habitat; (6) Restored 12 acres on the mainstem Walla Walla River and 32 acres on Couse Creek to native perennial grasses; and (7) Installed 50,000+ new native plants/cuttings within project areas.

  13. John Day River Sub-Basin Fish Habitat Enhancement Project; 1998 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Neal, Jeff A.; Jerome, James P.; Delano, Kenneth H.

    1999-02-01

    During 1998, three new projects were completed improving 1.8 miles of stream and riparian habitat. Protection for these reaches required the construction of 3.2 miles of riparian fence and 7 livestock water gaps. A previously leased property on the Mainstream was converted from apriarian pasture to a corridor fence after no significant recovery had occurred.

  14. Biodiversity in intertidal rock pools: informing engineering criteria for artificial habitat enhancement in the built environment.

    Science.gov (United States)

    Firth, Louise B; Schofield, Meredith; White, Freya J; Skov, Martin W; Hawkins, Stephen J

    2014-12-01

    Coastal defence structures are proliferating to counter rising and stormier seas. With increasing concern about the ecological value of built environments, efforts are being made to create novel habitat to increase biodiversity. Rock pools are infrequent on artificial structures. We compared biodiversity patterns between rock pools and emergent rock and assessed the role of pool depth and substratum incline in determining patterns of biodiversity. Rock pools were more taxon rich than emergent substrata. Patterns varied with depth and incline with algal groups being more positively associated with shallow than deeper habitats. Substratum incline had little influence on colonising epibiota, with the exception of canopy algae in deeper habitats where vertical surfaces supported greater taxon richness than horizontal surfaces. The creation of artificial rock pools in built environments will have a positive effect on biodiversity. Building pools of varying depths and inclines and shore heights will provide a range of habitats, increase environmental heterogeneity, therefore creating more possible ecological niches, promoting local biodiversity. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Walla Walla River Basin Fish Habitat Enhancement Project, 2001-2002 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Volkman, Jed; Sexton, Amy D. (Confederated Tribes of the Umatilla Indian Reservation, Pendleton, OR)

    2003-04-01

    In 2001, the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) Fisheries Habitat Program implemented stream habitat restoration and protection efforts in the Walla Walla River Basin with funding from Bonneville Power Administration (BPA). The objective of these efforts is to protect and restore habitat critical to the recovery of weak or reintroduced populations of salmonid fish. The CTUIR has currently enrolled six properties into this program: two on Couse Creek, two adjacent properties on Blue Creek, one on Patit Creek, and one property on the mainstem Walla Walla River. Since 1997, approximately 7 miles of critical salmonid habitat has been secured for restoration and protection under this project. Major accomplishments to date include the following: Secured approximately $250,000 in cost share; Secured 7 easements; Planted 30,000+ native plants; Installed 50,000+ cuttings; and Seeded 18 acres to native grass. Pre and post-project monitoring efforts were included for all projects, incorporating methodologies from CTUIR's Draft Monitoring Plan. Basin-wide monitoring also included the deployment of 6 thermographs to collect summer stream temperatures.

  16. FSH regulates acetycholine production by ovarian granulosa cells

    Directory of Open Access Journals (Sweden)

    Amsterdam Abraham

    2006-07-01

    Full Text Available Abstract Background It has been previously shown that cultured granulosa cells (GCs derived from human ovarian preovulatory follicles contain choline acetyltransferase (ChAT, the enzyme responsible for acetylcholine (ACh synthesis. They also produce ACh and express functional muscarinic ACh receptors. ACh can act on GCs to increase proliferation, disrupt gap junctional communication, alter intracellular calcium levels, as well as expression of transcription factors, suggesting an unrecognized role of ACh in GC function. To gain further insights into the possible role of ACh in the ovary, we examined ChAT expression in the gland before and after birth, as well as in adults, and studied the regulation of ACh production by FSH. Methods ChAT immunohistochemistry was performed using ovarian samples of different species and ages (embryonic, postnatal and adult rats and mice, including embryonic ovaries from mice null for ChAT, neonatal and adult rhesus monkeys and adult humans. ACh was measured by HPLC and/or a fluorescence based method in rat ovaries and in a FSH receptor-expressing cell line (rat GFSHR-17 cultured with or without FSH. Results In adult rat, as well as in all other species, ovarian ChAT immunoreactivity is associated with GCs of antral follicles, but not with other structures, indicating that GCs are the only ovarian source of ACh. Indeed ACh was clearly detected in adult rat ovaries by two methods. ChAT immunoreactivity is absent from embryonic and/or neonatal ovaries (mouse/rat and monkey and ovarian development in embryonic mice null for ChAT appears normal, suggesting that ACh is not involved in ovarian or follicular formation. Since ChAT immunoreactivity is present in GCs of large follicles and since the degree of the ChAT immunoreactivity increases as antral follicles grow, we tested whether ACh production is stimulated by FSH. Rat GFSHR-17 cells that stably express the FSH receptor, respond to FSH with an increase in ACh

  17. Thyrotropin-releasing hormone provokes abnormal follicle-stimulating hormone (FSH) and luteinizing hormone responses in men who have pituitary adenomas and FSH hypersecretion.

    Science.gov (United States)

    Snyder, P J; Muzyka, R; Johnson, J; Utiger, R D

    1980-10-01

    Serum FSH ad LH concentrations after the administration of TRH were measured in 10 men who had pituitary adenomas associated with FSH hypersecretion. Similar measurements were made in 12 men who had pituitary adenomas but no FSH hypersecretion, in 10 age-matched, normal men, and in 5 men who had primary hypergonadism. The mean serum LH concentration in the men who had pituitary adenomas and FSH hypersecretion increased 136% after TRH administration, significantly greataer (P < 0.005) than the 48% increase in the normal men or the 51% increase in the men who had pituitary adenomas without FSH hypersecretion. Serum LH did not increase at all in the men who had primary hypoganadism. The serum FSH concentration did not increase in any of the normal men, in the men who had pituitary adenomas without FSH hypersecretion, or in the men who had primary hypogonadism, but did increase in 5 of the 10 men who had FSH hypersecretion; the mean increase in these 5 men was 38%. The exaggerated LH responses and the nonspecific FSH responses to TRH of the men who had pituitary adenomas associated with FSH hypersecretion suggest that control of both FSH and LH secretion by these adenomas is abnormal and, therefore, that these adenomas are likely gonadotroph cell adenomas.

  18. Providing habitat for native mammals through understory enhancement in forestry plantations.

    Science.gov (United States)

    Simonetti, Javier A; Grez, Audrey A; Estades, Cristián F

    2013-10-01

    The Convention on Biological Diversity (CBD) expects forestry plantations to contribute to biodiversity conservation. A well-developed understory in forestry plantations might serve as a surrogate habitat for native species and mitigate the negative effect of plantations on species richness. We experimentally tested this hypothesis by removing the understory in Monterey pine (Pinus radiata) plantations in central Chile and assessing changes in species richness and abundance of medium-sized mammals. Frequency of occurrence of mammals, including kodkods (Leopardus guigna), culpeo foxes (Pseudalopex culpaeus), lesser grisons (Conepatus chinga), and Southern pudu deer (Pudu puda), was low in forest stands with little to no understory relative to stands with well-developed undergrowth vegetation. After removing the understory, their frequency of occurrence decreased significantly, whereas in control stands, where understory was not removed, their frequency did not change. This result strongly supports the idea that facilitating the development of undergrowth vegetation may turn forestry stands into secondary habitats as opposed to their containing no habitat for native mammals. This forestry practice could contribute to conservation of biological diversity as it pertains to CBD targets.

  19. Effects of Progestin and Antiprogestin on the Expression of FSH Receptor and LH Receptor mRNA in Porcine Granulosa and Thecal Cells

    Institute of Scientific and Technical Information of China (English)

    吴尔若; 刘德瑜; 赵金来; 吴燕婉

    2000-01-01

    In order to investigate the mechanism of progestin and antiprogestin in the regula-tion of ovarian steroidogenesis, a dual-chamber culture system was prepared with the amnion membrane of human placenta. Isolated porcine granulosa and thecal cells from 4~6 mm-diameter follicles were grown on both sides of the amnion, respectively, and co-cultured with or without LNG and RU486. After 48 h incubation, the mRNAs of FSH receptor (FSH-R) and LH receptor (LH-R) of both cells were observed by in situ hybridization. The results showed that granulosa cells expressed both FSH-R mR-NA and LH-R mRNA, while thecal cells expressed LH-R mRNA only. Under the stimulation of FSH, both LNG and RU486 increased FSH-R mRNA expression of granulosa cells. Under the stimulation of LH, LNG enhanced LH-R mRNA expres-sion of thecal cells;while RU486 decreased its expression. When granulosa and thecal cells were exposed to FSH and LH both, the actions of LNG and RU 486 in thecal cells showed the same result as that stimulated by LH alone. In granulosa cells LNG de-creased LH-R mRNA expression, while RU486 increased its expression. These data suggest that; (1) granulosa cells expressed FSH-R mRNA significantly; (2) both the progestin and antiprogestin directly acted on the mRNA expression of gonadotropin re-ceptors of ovarian cells, but effects were different; (3) the response of granulosa or thecal cells to the action of LNG and RU486 was not the same. The mechanism needs to be further investigated.

  20. Characterisation of Population Pharmacokinetics and Endogenous Follicle Stimulating Hormone (FSH) Levels after Multiple Dosing of a Recombinant Human FSH, FE 999049, in Healthy Women

    DEFF Research Database (Denmark)

    Rose, Trine Høyer; Roshammer, Daniel; Erichsen, Lars

    2016-01-01

    Objective: The aim of this study was to characterise the population pharmacokinetics of FE 999049, a novel recombinant human follicle-stimulating hormone (FSH), after multiple dosing in healthy women, taking into account endogenous FSH levels and the reproductive hormone dynamics. Methods......: Longitudinal measurements of FSH, luteinising hormone, progesterone, estradiol, and inhibin B levels were collected after repeated subcutaneous dosing with 225 IU of FE 999049 in 24 gonadotropin downregulated healthy women. The FSH data were described using nonlinear mixed-effects modelling. Results...

  1. Levels of FSH, LH and testosterone, and sperm DNA fragmentation.

    Science.gov (United States)

    Wdowiak, Artur; Raczkiewicz, Dorota; Stasiak, Magdalena; Bojar, Iwona

    2014-01-01

    Having an offspring is the most important human biological goal, which is necessary for survival of the human species. Lack of offspring is a phenomenon concerning approximately 15% of married couples in Poland. In a half of the cases, a causative agent is the male factor infertility problem. There is evidence that certain male fertility problems are related with disorders of the process of spermatogenesis. The course of normal spermatogenesis depends on proper pituitary secretion of folliculostimulin (FSH), luteinizing hormone (LH), as well as testicular secretion of testosterone. It is considered that in approximately 20% of patients with idiopathic infertility an elevated level of sperm DNA fragmentation may be the cause of failure in reproduction. The objective of the present study was determination of the relationship between FSH, LH and testosterone levels, and the occurrence of sperm DNA fragmentation. The present study was conducted in the year 2012 in the Non-Public Health Care Unit 'Ovum Reproduction and Andrology' in Lublin, and covered 186 men treated for infertility. For inclusion into the study group we qualified males aged 25-35, who have been treated for infertility for more than 1 year, with no pathological features observed in the female partner. The structure of sperm chromatin was evaluated using the technique of flow cytometry-Sperm Chromatin Structure assay (SCSA). The result of the examination was a sperm DNA Fragmentation Index (DFI), i.e., the percentage of sperm with DNA lesions (DNA fragmentation). A morning blood sample (5 mL volume) was obtained and sent to an authorized laboratory to assess serum levels of testosterone, LH and FSH. An intensified sperm DNA fragmentation co-occurred with both extremely low and extremely high levels of FSH and LH. Sperm DNA fragmentation was negatively correlated with testosterone level.

  2. Incorporating a sorghum habitat for enhancing lady beetles (Coleoptera:Coccinellidae) in cotton

    Science.gov (United States)

    Lady beetles (Coleoptera: Coccinellidae) are important predators of cotton insect pests. The objective of this 2-yr on-farm study was to examine the ability of a sorghum trap crop with Euschistus spp. pheromone baited capture traps to enhance these predators in cotton in Georgia. Scymnus spp., Cocci...

  3. Cover Image Identification of Plant Species for Crop Pollinator Habitat Enhancement in the Northern Prairies

    Directory of Open Access Journals (Sweden)

    Diana Bizecki Robson

    2014-09-01

    Full Text Available Wild pollinators have a positive impact on the productivity of insect-pollinated crops. Consequently, landowners are being encouraged to maintain and grow wildflower patches to provide habitat for important pollinators. Research on plant-pollinator interaction matrices indicates that a small number of “core” plants provide a disproportionately high amount of pollen and nectar to insects. This matrix data can be used to help design wildflower plantings that provide optimal resources for desirable pollinators. Existing interaction matrices from three tall grass prairie preserves in the northern prairies were used to identify core plant species that are visited by wild pollinators of a common insect-pollinated crop, namely canola (Brassica napus L.. The wildflower preferences of each insect taxon were determined using quantitative insect visitation and floral abundance data. Phenology data were used to calculate the degree of floral synchrony between the wildflowers and canola. Using this information I ranked the 41 wildflowers that share insect visitors with canola according to how useful they are for providing pollinators with forage before and after canola flowers. The top five species were smooth blue aster (Symphyotrichum laeve (L. A. & D. Löve, stiff goldenrod (Solidago rigida L., wild bergamot (Monarda fistulosa L., purple prairie-clover (Dalea purpurea Vent. and Lindley’s aster (Symphyotrichum ciliolatum (Lindl. A. & D. Löve. By identifying the most important wild insects for crop pollination, and determining when there will be “pollen and nectar gaps”, appropriate plant species can be selected for companion plantings to increase pollinator populations and crop production.

  4. Predicted effect of landscape position on wildlife habitat value of Conservation Reserve Enhancement Program wetlands in a tile-drained agricultural region

    Science.gov (United States)

    Otis, David L.; Crumpton, William R.; Green, David; Loan-Wilsey, Anna; Cooper, Tom; Johnson, Rex R.

    2013-01-01

    Justification for investment in restored or constructed wetland projects are often based on presumed net increases in ecosystem services. However, quantitative assessment of performance metrics is often difficult and restricted to a single objective. More comprehensive performance assessments could help inform decision-makers about trade-offs in services provided by alternative restoration program design attributes. The primary goal of the Iowa Conservation Reserve Enhancement Program is to establish wetlands that efficiently remove nitrates from tile-drained agricultural landscapes. A secondary objective is provision of wildlife habitat. We used existing wildlife habitat models to compare relative net change in potential wildlife habitat value for four alternative landscape positions of wetlands within the watershed. Predicted species richness and habitat value for birds, mammals, amphibians, and reptiles generally increased as the wetland position moved lower in the watershed. However, predicted average net increase between pre- and post-project value was dependent on taxonomic group. The increased average wetland area and changes in surrounding upland habitat composition among landscape positions were responsible for these differences. Net change in predicted densities of several grassland bird species at the four landscape positions was variable and species-dependent. Predicted waterfowl breeding activity was greater for lower drainage position wetlands. Although our models are simplistic and provide only a predictive index of potential habitat value, we believe such assessment exercises can provide a tool for coarse-level comparisons of alternative proposed project attributes and a basis for constructing informed hypotheses in auxiliary empirical field studies.

  5. Discordances between follicle stimulating hormone (FSH and anti-Müllerian hormone (AMH in female infertility

    Directory of Open Access Journals (Sweden)

    Weghofer Andrea

    2010-06-01

    Full Text Available Abstract Background Follicle stimulating hormone (FSH and anti-Müllerian hormone (AMH represent the two most frequently utilized laboratory tests in determining ovarian reserve (OR. This study determined the clinical significance of their concordance and discordance in female infertility patients. Methods We investigated 366 consecutive infertility patients (350 reached IVF, excluding women with polycystic ovarian syndrome (PCOS. They were considered to have normal FSH and AMH if values fell within age-specific (as- 95% confidence intervals (CI, and to suffer from diminished ovarian reserve (DOR if FSH exceeded and/or AMH fell below those. The two hormones, thus, could be concordant (Group I, both normal (IA or abnormal (IB, show normal AMH/abnormal FSH (Group II or normal FSH/abnormal AMH (Group III. Oocyte yields, stratified for age categories, were then studied in each group as reflection of OR. Results Oocyte yields significantly decreased from groups IA to II to III and IB. Predictive values of as-FSH/AMH patterns changed, however, at different ages. Except at very young and very old ages, normal as-AMH better predicted higher oocytes yields than normal as-FSH, though above age 42 years normal as-FSH predicts good oocyte yields even with abnormally low AMH. Under age 42 discrepancies between as- FSH and as-AMH remain similarly predictive of oocyte yields at all ages. Discussion Concordances and discordances between as-FSH and as-AMH improve OR assessments and predictability of oocyte yields in IVF.

  6. Fsh controls gene expression in fish both independently of and through steroid mediation.

    Directory of Open Access Journals (Sweden)

    Elisabeth Sambroni

    Full Text Available The mechanisms and the mediators relaying Fsh action on testicular functions are poorly understood. Unlike in mammals, in fish both gonadotropins (Fsh and Lh are able to efficiently stimulate steroidogenesis, likely through a direct interaction with their cognate receptors present on the Leydig cells. In this context, it is crucial to understand if Fsh effects are mediated through the production of steroids. To address this issue we performed transcriptome studies after in vitro incubations of rainbow trout testis explants in the presence of Fsh alone or in combination with trilostane, an inhibitor of Δ4- steroidogenesis. Trilostane significantly reduced or suppressed the response of many genes to Fsh (like wisp1, testis gapdhs, cldn11, inha, vt1 or dmrt1 showing that, in fish, important aspects of Fsh action follow indirect pathways and require the production of Δ4-steroids. What is more, most of the genes regulated by Fsh through steroid mediation were similarly regulated by Lh (and/or androgens. In contrast, the response to Fsh of other genes was not suppressed in the presence of trilostane. These latter included genes encoding for anti-mullerian hormone, midkine a (pleiotrophin related, angiopoietine-related protein, cyclins E1 and G1, hepatocyte growth factor activator, insulin-like growth factor 1b/3. A majority of those genes were preferentially regulated by Fsh, when compared to Lh, suggesting that specific regulatory effects of Fsh did not depend on steroid production. Finally, antagonistic effects between Fsh and steroids were found, in particular for genes encoding key factors of steroidogenesis (star, hsd3b1, cyp11b2-2 or for genes of the Igf system (igf1b/3. Our study provides the first clear evidence that, in fish, Fsh exerts Δ4-steroid-independent regulatory functions on many genes which are highly relevant for the onset of spermatogenesis.

  7. Heterogeneity of rat FSH by chromatofocusing: studies on in-vitro bioactivity of pituitary FSH forms and effect of neuraminidase treatment.

    Science.gov (United States)

    Blum, W F; Riegelbauer, G; Gupta, D

    1985-04-01

    This study concerned the resolution of rat pituitary FSH utilizing chromatofocusing. Among the 11 components resolved and positively identified, ten had apparent isoelectric points (pI) between 3.1 and 5.1. Approximately 1% of pituitary FSH eluted at pH 9.4. Treatment with varying amounts of neuraminidase followed by refocusing generated FSH components of higher pI values. Treatment with other glycosidases did not alter the elution characteristics in chromatofocusing, while exclusion chromatography established an inverse relationship between apparent molecular weight and pI. Dose-response curves of various FSH components and of the reference preparation in the current radioimmunoassay system were parallel to each other. A study of their in-vitro bioactivity, utilizing granulosa cells which produce a plasminogen activator due to FSH in a dose-dependent manner, provided the following evidence: increased acidity of the components led to an increase of maximum response and an increase of the dose necessary for half-maximum response. Considering the observed alterations in the heterogeneity of FSH with changing physiological states of the animal, it is concluded that qualitative changes of the FSH molecule are perhaps involved in a modulatory role in the biopotencies of the hormone.

  8. Comparison of Specificity and Sensitivity of AMH and FSH in Diagnosis of Premature Ovarian Failure

    Directory of Open Access Journals (Sweden)

    Farzaneh Alipour

    2015-01-01

    Full Text Available Introduction. Anti-Müllerian hormone represents the primitive follicular number and ovarian age. Low level of AMH is in relation to early menopausal state and decreased ovarian reserve. AMH level changes occur prior to FSH level in representing ovarian failure. The aim of this study is to compare sensitivity and specificity of AMH with FSH in diagnosis of POF. Material and Methods. This descriptive study is done on 96 patients referred to Dr. Rasekh Clinic. Serum level of AMH and FSH was measured at Day 3 (3rd day of menstrual cycle and data were analyzed through SPSS 21 software. Results. Results of AMH and FSH serum level indicate that AMH has more sensitivity (80% versus 28.57% and almost equal specificity (78.89% versus 78.65% compared with FSH. Also negative predictive value of AMH (98.61% and FSH (87.5% is different. But positive predictive value is the same (17.39%. Diagnostic accuracy of AMH is more than FSH and has significant differences. Conclusion. According to the results of this study, AMH serum level is more sensitive than FSH serum level. Also AMH has more negative predictive value. Besides, this hormone can be measured at any time of menstrual cycle, against FSH. AMH seems to be more useful in early diagnosis of POF.

  9. Age-specific reference values for serum FSH and estradiol levels throughout the reproductive period.

    Science.gov (United States)

    Grisendi, Valentina; Spada, Elena; Argento, Cindy; Plebani, Maddalena; Milani, Silvano; Seracchioli, Renato; Volpe, Annibale; La Marca, Antonio

    2014-06-01

    High serum day 3 FSH levels are associated with poor ovarian reserve and reduced fertility, but the interpretation of FSH values according to age is still not univocal. The purpose of this study was to determine age-dependent reference values in women with regular menstrual cycles and FSH as a guide for specialists. The study was performed at the Department of Mother-Infant of a University-based tertiary care centre. One-hundred ninety-two healthy normal menstruating women were recruited for the study. All patients attended the department on menstrual cycle day 3 for a blood sample for FSH and estradiol determination. A linear relationship between FSH or estradiol serum levels and age was observed. The FSH level increased by 0.11 IU for every year of age (1 IU for every 9 years of age). The values of FSH and estradiol corresponding to the 5th, 25th, 50th, 75th, 95th centiles for any specific age have been calculated. Serum FSH levels need to be interpreted according to age-dependent reference values. Serum FSH levels on 95th centile for any age may represent a warning sign for reduced ovarian reserve.

  10. Individualization of the FSH starting dose in IVF/ICSI cycles using the antral follicle count

    Directory of Open Access Journals (Sweden)

    La Marca Antonio

    2013-02-01

    Full Text Available Abstract Background The FSH starting dose is usually chosen according to women’s age, anamnesis, clinical criteria and markers of ovarian reserve. Currently used markers include antral follicle count (AFC, which is considered to have a very high performance in predicting ovarian response to FSH. The objective of the present study to elaborate a nomogram based on AFC for the calculation of the appropriate FSH starting dose in IVF cycles. Methods This is a retrospective study performed at the Mother-Infant Department of Modena University Hospital. IVF patients (n=505 were subjected to blood sampling and transvaginal ultrasound for measurement of serum day3 FSH, estradiol and AFC. The variables predictive of the number of retrieved oocytes were assessed by backwards stepwise multiple regression. The variables reaching the statistical significance were then used in the calculation for the final predictive model. Results A model based on age, AFC and FSH was able to accurately predict the ovarian sensitivity and accounted for 30% of the variability of ovarian response to FSH. An FSH dosage nomogram was constructed and overall it predicts a starting dose lower than 225 IU in 50.2% and 18.1% of patients younger and older than 35 years, respectively. Conclusions The daily FSH dose may be calculated on the basis of age and two markers of ovarian reserve, namely AFC and FSH, with the last two variables being the most significant predictors. The nomogram seems easily applicable during the daily clinical practice.

  11. Tingkat Aktivitas Sel Endokrin Penghasil Folikel Stimulating Hormon (FSH Terkait Pemberian Pasak Bumi (Eurycoma longifolia JACK

    Directory of Open Access Journals (Sweden)

    Hurip Pratomo

    2015-05-01

    Full Text Available Pasak bumi is popular as an aphrodisiac. Traditionally, pasak bumi infusion is used to increase libido and duration of erection. The research was focused on: Activity level of FSH cells after three days of pasak bumi treatment. The research was carried out by evaluation using immunohistochemistry method via antibody anti FSH staining. The result of the research showed that a dosage of 18 mg/200 g body weight (bw orally of pasak bumi maintained certain endoceine positive cells e.g. FSH production cells and were found stabile in the third days. Pasak bumi serves a stabilizer for intracellular levels of FSH in the anterior hipophysis/pituatary.

  12. A Wildlife Habitat Protection, Mitigation and Enhancement Plan for Eight Federal Hydroelectric Facilities in the Willamette River Basin: Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Preston, S.K.

    1987-05-01

    The development and operation of eight federal hydroelectric projects in the Willamette River Basin impacted 30,776 acres of prime wildlife habitat. This study proposes mitigative measures for the losses to wildlife and wildlife habitat resulting from these projects, under the direction of the Columbia River Basin (CRB) Fish and Wildlife Program. The CRB Fish and Wildlife Program was adopted in 1982 by the Northwest Power Planning Council, pursuant to the Northwest Power Planning Act of 1980. The proposed mitigation plan is based on the findings of loss assessments completed in 1985, that used a modified Habitat Evaluation Procedure (HEP) to assess the extent of impact to wildlife and wildlife habitat, with 24 evaluation species. The vegetative structure of the impacted habitat was broken down into three components: big game winter range, riparian habitat and old-growth forest. The mitigation plan proposes implementation of the following, over a period of 20 years: (1) purchase of cut-over timber lands to mitigate, in the long-term, for big game winter range, and portions of the riparian habitat and old-growth forest (approx. 20,000 acres); (2) purchase approximately 4,400 acres of riparian habitat along the Willamette River Greenway; and (3) three options to mitigate for the outstanding old-growth forest losses. Monitoring would be required in the early stages of the 100-year plan. The timber lands would be actively managed for elk and timber revenue could provide O and M costs over the long-term.

  13. Evaluation of a small beach nourishment project to enhance habitat suitability for horseshoe crabs

    Science.gov (United States)

    Jackson, N.L.; Smith, D.R.; Tiyarattanachai, R.; Nordstrom, K.F.

    2007-01-01

    This field study evaluates the effect of nourishing an estuarine beach with gravel to enhance spawning rates by horseshoe crabs. A total of 564??m3 of coarse sand and gravel were emplaced in two 90??m-long treatment segments at Bowers Beach, Delaware, USA from 9 to 11 April 2002. Field data were gathered between 6 April and 24 May 2002 to characterize the two fill segments and the un-nourished segments between them as well as two control segments at the adjacent Ted Harvey Beach. Sediment samples were taken from the foreshore surface and at depth before and after the nourishment. Bay water levels, wave heights, and beach ground water characteristics were monitored over a 12-hour tidal cycle at one of the nourished (15 May 2002) and the unnourished segment (16 May 2002) at Bowers Beach and at one of the control segments at Ted Harvey Beach (21 May 2002) using piezometers and pressure transducers inserted in wells. The beaches were cored to estimate the density of horseshoe crab eggs deposited during the spawning season. Horseshoe crab eggs were buried in pouches at 0.15 to 0.20??m depth for 30 to 40??days to evaluate their survival in developing into embryo or larval stage. Bulk sediment samples were taken to evaluate moisture characteristics near locations where egg pouches were buried. Density of spawning females at Bowers Beach was 1.04??m- 2 in 2001 and 1.20??m- 2 in 2002. These rates are lower than at Ted Harvey Beach but reveal an increase in spawning while Ted Harvey Beach underwent a considerable decrease (2.63??m- 2 to 1.35??m- 2). Sediments low on the foreshore remained nearly saturated throughout the tidal cycle at both beaches. The average hydraulic conductivity on the upper foreshore at the non-treatment section at Bowers Beach (0.19??cm s- 1) was less than at Ted Harvey Beach (0.27??cm s- 1), and the finer, better sorted sediments at depth at Bowers Beach resulted in a higher porosity, creating greater moisture retention potential. Egg development was

  14. FSH and LH Secretion from in-vitro Cultured Buffalo Anterior Pituitary Cells Following Treatment with Diethyl-Stilbestrol and Medroxy-Progesterone and Their Effects on Ovarian Activity and Hematological Variables of Female Rabbits

    Directory of Open Access Journals (Sweden)

    Kaleem Iqbal1, Nafees Akhtar1*, Nazir Ahmad1 and Sajjad-ur-Rahman2

    2016-11-01

    Full Text Available Aims of this study were: to investigate whether FSH and LH secretion from in-vitro cultured buffalo adenohypophyseal cells can be increased by supplementing culture media with diethyl-stilbestrol and medroxy-progesterone, respectively; to monitor bioactivity of these in-vitro produced gonadotropins and to see if these gonadotropins have any adverse effects on hematology and internal body organs of female rabbits. Pituitary glands collected from 36 adult buffaloes slaughtered at a local abattoir were used. The anterior pituitary cells were cultured in-vitro using medium RPMI-1640 (code R6504-Sigma enriched with 10% fetal calf serum and GnRH and treated with 0.5 or 1.0 mg/100 ml diethyl-stilbestrol, and 2.5 or 5.0 mg/ml medroxy-progesterone, or left as untreated control. The results showed that FSH and LH concentrations from cultures treated with low or high dose of respective steroids were higher (P<0.05 than those for controls. Treatment of pre-pubertal female rabbits with in-vitro extracted FSH increased serum FSH and LH concentrations, ovarian size and number of developing follicles (GFs on the ovaries compared to controls (P<0.01. However, rabbits treated with in-vitro produced extract of LH showed increased serum FSH and LH, while there was no effect on ovarian size and number of GFs. Moreover, treatment of rabbits with both gonadotropins had no effects on body weight, hematological variables and internal body organs. In conclusion, diethyl-stilbestrol and medroxy-progesterone enhanced the secretion of FSH and LH, respectively, from cultured pituitary cells. Moreover, in-vitro produced FSH increased ovarian size, serum FSH and LH and stimulated ovarian activity, while in-vitro produced LH neither increased ovarian size nor stimulated ovarian activity.

  15. Sequential Versus Continual Purified Urinary FSH/hCG in Men With Idiopathic Hypogonadotropic Hypogonadism.

    Science.gov (United States)

    Zhang, Manna; Tong, Guoyu; Liu, Yanling; Mu, Yiming; Weng, Jianping; Xue, Yaoming; Luo, Zuojie; Xue, Yuanming; Shi, Lixin; Wu, Xueyan; Sun, Shouyue; Zhu, Yanhua; Cao, Ying; Zhang, Jie; Huang, Hong; Niu, Ben; Li, Hong; Guo, Qinghua; Gao, Yan; Li, Zhibin; Ning, Guang; Zhu, Dalong; Li, Xiaoying

    2015-06-01

    Gonadotropin therapy using a human chorionic gonadotropin (hCG) and FSH preparation is an effective regimen in inducing masculinization and spermatogenesis in men with idiopathic hypogonadotropic hypogonadism (IHH). However, the high cost of medication and frequent injections affect compliance. The aim of this study was to determine the efficacy of sequential use of highly purified urinary FSH (uFSH)/hCG in men with IHH. A randomized, open-label, prospective, controlled noninferiority trial with an 18-month follow-up was conducted in 9 tertiary hospitals. A total of 67 Chinese men with IHH were randomly allocated into group A receiving continual uFSH (75 U, 3 times a week) and hCG (2000 U, twice a week) injection and group B receiving sequential uFSH (75 U, 3 times a week every other 3 months) and hCG (2000 U, twice a week) injection. The primary outcome was the proportion of subjects with a sperm concentration of ≥ 1.0 × 10(6)/mL during the 18 months. The efficacy between groups A and B was compared for noninferiority. Of the patients, 17/33 (51.5%) receiving continual uFSH/hCG and 19/34 (55.9%) receiving sequential uFSH/hCG achieved sperm concentrations of ≥ 1.0 × 10(6)/mL. The efficacy in the sequential uFSH/hCG group was not inferior to that in the continual uFSH/hCG group (noninferiority, P = .008) by intention-to-treat analysis. The efficacy of the sequential uFSH/hCG regimen is not inferior to that of the continual uFSH/hCG regimen in inducing spermatogenesis and masculinization of patients with IHH.

  16. Macroorchidism in a Patient with FSH-Secreting Pituitary Macroadenoma

    Directory of Open Access Journals (Sweden)

    Eren Demirtaş

    2012-12-01

    Full Text Available Follicle-stimulating hormone-secreting pituitary macroadenoma (FSHoma is a very rare and usually asymptomatic entity. FSHoma can clinically present as ovarian hyperstimulation syndrome in female patients, but symptoms and clinical signs are usually not observed in males. An increase in testicular volume has been reported in male patients with FSHoma. In some male patients, the first finding may be macroorchidism. A 20-year-old male patient presented to our clinic due to enlarged testes (testicular volume: 45 cc. Scrotal ultrasonography showed macroorchidism. Tests required to determine the etiology of macroorchidism showed that the patient had an elevated FSH concentration. Macroadenoma was diagnosed based on pituitary magnetic resonance imaging. FSHoma was suspected and transsphenoidal resection was performed. Pathology test results indicated FSHoma. The present case shows that FSHoma is very rare and that it can present in association with macroorchidism in male patients. Turk Jem 2012; 16: 95-8

  17. Anti-Mullerian hormone attenuates the effects of FSH on follicle development in the mouse ovary

    NARCIS (Netherlands)

    A.L.L. Durlinger (Alexandra); A.P.N. Themmen (Axel); M.J.G. Gruijters (Maria); P. Kramer; B. Karels (Bas); T.R. Kumar (Rajendra); M.M. Matzuk; U.M. Rose; F.H. de Jong (Frank); J.Th.J. Uilenbroek (Jan); J.A. Grootegoed (Anton)

    2001-01-01

    textabstractAlthough ovarian follicle growth is under the influence of many growth factors and hormones of which FSH remains one of the most prominent regulators. Therefore, factors affecting the sensitivity of ovarian follicles to FSH are also important for follicle growth. The ai

  18. Identifying real differences in live birth rates between HMG and rFSH in IVF.

    Science.gov (United States)

    Afnan, Masoud

    2009-01-01

    Fertility treatment strives for the delivery of a healthy live birth. Human menopausal gonadotrophin (HMG) and recombinant FSH (rFSH) are the two types of gonadotrophin currently used for ovarian stimulation in assisted reproduction treatments. Although both HMG and rFSH have been shown to be effective, a number of studies have examined whether a potential difference in clinical benefit or outcome exists between treatments. Unlike rFSH preparations, HMG contains both FSH and LH activity (in the form of LH and human chorionic gonadotrophin, which are short- and long-acting, respectively). The beneficial effect of exogenous LH activity has been investigated in the Menotrophin versus Recombinant FSH in-vitro Fertilisation Trial (MERiT), which revealed differences in embryo quality and endometrial receptivity between rFSH and highly purified HMG. Current evidence suggests that HMG provides significantly higher live birth rates than rFSH in women undergoing ovarian stimulation for in-vitro fertilization/intracytoplasmic sperm injection cycles using long gonadotrophin-releasing hormone agonist protocol. Further studies will continue to provide data with which to expand these findings and optimize the chances of achieving a live birth following assisted reproduction treatment.

  19. Genetically determined dosage of follicle-stimulating hormone (FSH) affects male reproductive parameters

    DEFF Research Database (Denmark)

    Grigorova, Marina; Punab, Margus; Zilaitienė, Birutė

    2011-01-01

    The detailed role of FSH in contributing to male testicular function and fertility has been debated. We have previously identified the association between the T-allele of the FSHB promoter polymorphism (rs10835638; G/T, -211 bp from the mRNA start) and significantly reduced male serum FSH....

  20. Comparative pharmacology of a new recombinant FSH expressed by a human cell Line

    DEFF Research Database (Denmark)

    Koechling, Wolfgang; Plaksin, Daniel; Croston, Glenn

    2017-01-01

    Recombinant FSH proteins are important therapeutic agents for the treatment of infertility, including follitropin alfa expressed in Chinese Hamster Ovary (CHO) cells and, more recently, follitropin delta expressed in the human cell line PER.C6. These recombinant FSH proteins have distinct...

  1. Iskuulpa Watershed Management Plan : A Five-Year Plan for Protecting and Enhancing Fish and Wildlife Habitats in the Iskuulpa Watershed.

    Energy Technology Data Exchange (ETDEWEB)

    Confederated Tribes of the Umatilla Indian Reservation Wildlife Program

    2003-01-01

    The Confederated Tribes of the Umatilla Indian Reservation (CTUIR) propose to protect, enhance, and mitigate wildlife and wildlife habitat and watershed resources in the Iskuulpa Watershed. The Iskuulpa Watershed Project was approved as a Columbia River Basin Wildlife Fish and Mitigation Project by the Bonneville Power Administration (BPA) and Northwest Power Planning Council (NWPPC) in 1998. Iskuulpa will contribute towards meeting BPA's obligation to compensate for wildlife habitat losses resulting from the construction of the John Day and McNary Hydroelectric facilities on the Columbia River. By funding the enhancement and operation and maintenance of the Iskuulpa Watershed, BPA will receive credit towards their mitigation debt. The purpose of the Iskuulpa Watershed management plan update is to provide programmatic and site-specific standards and guidelines on how the Iskuulpa Watershed will be managed over the next three years. This plan provides overall guidance on both short and long term activities that will move the area towards the goals, objectives, and desired future conditions for the planning area. The plan will incorporate managed and protected wildlife and wildlife habitat, including operations and maintenance, enhancements, and access and travel management.

  2. Highly purified HMG versus recombinant FSH for ovarian stimulation in IVF cycles

    DEFF Research Database (Denmark)

    Platteau, P.; Nyboe, Andersen A.; Loft, A.;

    2008-01-01

    The objective of this study was to compare the live birth rates resulting from ovarian stimulation with highly purified human menopausal gonadotrophin (HP-HMG), which combines FSH and human chorionic gonadotrophin-driven LH activities, or recombinant FSH (rFSH) alone in women undergoing IVF cycles....... An integrated analysis was performed of the raw data from two randomized controlled trials that were highly comparable in terms of eligibility criteria and post-randomization treatment regimens with either HP-HMG or rFSH for ovarian stimulation in IVF, following a long down-regulation protocol. All randomized...... subjects who received at least one dose of gonadotrophin in an IVF cycle (HP-HMG, n = 491; rFSH, n = 495) were included in the analysis. Subjects who underwent intracytoplasmic sperm injection cycles were excluded. The superiority of one gonadotrophin preparation over the other was tested using...

  3. Producing of bovine follicle stimulating hormone (bFSH) by genetic engineering

    Institute of Scientific and Technical Information of China (English)

    艾秀莲; 陈新国; 季青; 龙涛; 余红; 昔奋攻; 董平; 石玉瑚

    1995-01-01

    A cDNA library was prepared from mRNA extracted from bovine pituitaries,β-subunitDNA of bFSH was amplified from cDNA using Polymerase Chain Reaction(PCR).The sequence of aminoacid encoded by cDNA was the same as that of natural bFSH by sequencing.Signal peptide DNAfragment of bFSH was deleted by PCR mediated mutagenesis,to which PGEX-2T was ligated,yielding a high expression fusion protein of 40 kD.A recombinant bFSH fusion protein was extracted usingthrombin,yielding a pure bFSH of 14kD,showing positive reaction using Western blot analysis,and someactivation by biological tests with the mouse and the rabbit.

  4. Chromatofocusing fails to separate hFSH isoforms on the basis of glycan structure.

    Science.gov (United States)

    Bousfield, George R; Butnev, Vladimir Y; Bidart, Jean-Michel; Dalpathado, Dilusha; Irungu, Janet; Desaire, Heather

    2008-02-12

    Follicle-stimulating hormone (FSH) glycosylation is regulated by feedback from the gonads, resulting in an array of glycans associated with FSH preparations derived from pools of pituitary or urine extracts. FSH glycosylation varies due to inhibition of FSHbeta N-glycosylation, elaboration of 1-4 branches possessed by mature N-glycans, and the number and linkage of terminal sialic acid residues. To characterize FSH glycosylation, FSH isoforms in pituitary gland extracts and a variety of physiological fluids are commonly separated by chromatofocusing. Variations in the ratios of immunological and biological activities in the resulting FSH isoform preparations are generally attributed to changes in glycosylation, which are most often defined in terms of sialic acid content. Using Western blotting to assess human FSHbeta glycosylation inhibition revealed 30-47% nonglycosylated hFSHbeta associated with four of six hFSH isoform preparations derived by chromatofocusing. Glycopeptide mass spectrometry assessment of glycan branching in these isoforms extensively characterized two N-glycosylation sites, one at alphaAsn52, the critical glycan for FSH function, and the other at betaAsn24. With two to four N-glycans per FSH molecule, many combinations of charges distributed over these sites can provide the same isoelectric point. Indeed, several glycans were common to all isoform fractions that were analyzed. There was no trend showing predominantly monoantennary glycans associated with the high-pI fractions, nor were predominantly tri- and tetra-antennary glycans associated with low-pI fractions. Thus, differences in receptor binding activity could not be associated with any specific glycan type or location in the hormone. FSH aggregation was associated with reduced receptor binding activity but did not affect immunological activity. However, as gel filtration indicated sufficient heterodimer was present in each isoform preparation to generate complete inhibition curves, the

  5. EFFECT OF FSH β-SUB UNIT AND FSHR GENES POLYMORPHISMS ON SUPEROVULATORY RESPONSE TRAITS

    Directory of Open Access Journals (Sweden)

    E. Andreas

    2015-09-01

    Full Text Available Follicle stimulating hormone (FSH is a pituitary expressed glycoprotein hormone that regulatesreproduction in mammals which composed of α and β-sub unit. The β-sub unit dictates its bindingspecificity with their receptor (FSHR. This study aimed to identify polymorphism of FSH β-sub unitand FSHR genes, and its effect to superovulatory response traits on superovulated cows. Study was doneon 32 cows including Angus, Friesian Holstein (FH, Limousin, Simmental and Brahman in CipelangLivestock Embryo Center. Cows used have been treated superovulation and mated by artificialinsemination. Superovulation response (SR, ovulation rate (OR, fertilization percentage (FP andviable transfer embryo percentage (VP were analyzed to investigate the effect of FSH β-sub unit andFSHR polymorphism. Allele frequency of FSH β-sub unit|PstI and FSH|AluI were opposite withinspecies. Mostly B allele and C allele for FSH β-sub unit and FSHR respectively have a high number inBos taurus species while those were in contrast in Bos indicus species. The highest heterozygosity wasfound in FH cattle (0.250 for FSH β-sub unit and Brahman (0.333 for FSHR. Significant effect was found between FSHR gene polymorphism with ovulation rate where CC genotype was higher (P<0.05than CG and GG genotypes.

  6. FSH and bFGF stimulate the production of glutathione in cultured rat Sertoli cells.

    Science.gov (United States)

    Gualtieri, Ariel F; Mazzone, Graciela L; Rey, Rodolfo A; Schteingart, Helena F

    2009-06-01

    Migration of developing germ cells from the basal to the adluminal compartment of the seminiferous epithelium requires extensive tissue restructuring, resulting in the production of reactive oxygen species. Sertoli cells are involved in this process. Glutathione (GSH), produced by Sertoli cells, has an essential role in cell protection against oxidative stress. Intracellular GSH content is maintained by de novo synthesis, involving glutamate-cysteine ligase catalytic (GCLC) and modulatory (GCLM) subunits, and by recycling from oxidized GSH, catalysed by glutathione reductase (GR). To assess whether follicle-stimulating hormone (FSH) and basic fibroblast growth factor (bFGF) modulate GSH production in Sertoli cells by regulating the expression of GCLC, GCLM and/or GR, we performed in vitro studies using rat Sertoli cells in primary culture. FSH and bFGF stimulation increased Sertoli cell GSH levels after 24 h incubation. The simultaneous addition of FSH and bFGF did not produce any further effect. GCLM expression was upregulated by FSH and bFGF 6 h. At 24 h, only the FSH-mediated effect was still observed. FSH and bFGF also upregulated GR expression. In conclusion, our results show that FSH and bFGF increase GSH levels in Sertoli cells through stimulation of the de novo synthesis and recycling by upregulating GCLM and GR expression respectively. Therefore, protection of germ cells against oxidative stress seems to be regulated by hormones and germ cell-released growth factors capable of influencing the production of Sertoli cell GSH.

  7. The effectiveness of habitat modification schemes for enhancing beneficial insects: Assessing the importance of trap cropping management approach

    Science.gov (United States)

    Trisnawati, Indah; Azis, Abdul

    2017-06-01

    Many farms in regions of intensive crop production lack the habitats that historically provided resources to beneficial insects, and this lack has compromised the ability of farmers to rely on natural enemies for pest control. One of the strategies to boost populations of existing or naturally occurring beneficial insects is to supply them with appropriate habitat and alternative food sources, such as diversifying trap crop systems and plant populations in or around fields include perennials and flowering plants. Trap cropping using insectary plant that attracts beneficial insects as natural enemies, especially flowering plants, made for provision of habitat for predators or parasitoids that are useful for biological control. Perimeter trap cropping (PTC) is a method of integrated pest management in which the main crop is surrounded with a perimeter trap crop that is more attractive to pests. We observed PTC habitat modification and conventionaly-managed tobacco farms in Purwosari Village, Pasuruan (East Java) to evaluate the effectiveness of habitat modification management prescription (perimeter trap crop using flowering plant Crotalaria juncea) on agroecosystem natural enemies. Field tests were conducted in natural enemies (predator and parasitoid) abundance dynamic and diversity on tobacco field in Purwoasri, Pasuruan. Yellow pan trap, sweep net and hand collecting methods were applied in each 10 days during tobacco growth stage (vegetative, generative until reproductive/harvesting. The results showed that application perimeter trap crop with C. juncea in tobacco fields able to help arthropod conservation of natural enemies on all tobacco growth stages. These results were evidenced the increase in abundance of predators and parasitoids and the increased value of the Diversity Index (H') and Evenness Index (EH) in all tobacco growth phases. Composition of predator and parasitoid in the habitat modification field were more diverse than in the conventional field

  8. During seminiferous tubule maturation testosterone and synergistic action of FSH with estradiol support germ cell survival while estradiol alone has pro-apoptotic effect.

    Directory of Open Access Journals (Sweden)

    Katarzyna Marchlewsk

    2008-04-01

    Full Text Available During establishment of spermatogenesis at the prepubertal age, an early germ cells apoptotic wave occurs likely aimed to remove abnormal germ cells and to maintain a proper cell number ratio between maturating germ cells and Sertoli cells. Here we assessed Sertoli and germ cell apoptosis in relation to morphological parameters of Sertoli cell maturation in neonatal rats under the influence of testosterone, estradiol and FSH given alone or in combinations. From postnatal day (PND 5th to 15th male rats were daily injected with: 1 2.5 mg of testosterone propionate (TP, or 2 12.5 microg of 17beta-estradiol benzoate (EB, or 3 TP+EB, or 4 7.5 IU of human purified FSH (hFSH, or 5 hFSH+EB or solvents (control-C. Autopsy was performed on PND 16th. Sertoli cell nuclei area and incidence of seminiferous tubule lumen formation (LF were taken as markers of Sertoli cell maturation. Sertoli and germ cell apoptosis was assessed using TUNEL method. In comparison with C, the area of Sertoli cell nuclei was significantly reduced after EB (25.7+/-2.0 vs. 30.9+/-1.6 microm2 for C, p<0.001 and increased after hFSH+EB (33.1+/-2.3 microm2, p<0.05. Incidence of LF was completely arrested by steroid hormone treatments given separately, significantly inhibited after TP+EB (median: 0.0%, vs. 2.0% for C p<0.05 and significantly enhanced after hFSH+EB (median: 51.0%, p<0.001. hFSH alone did not influence LF. Incidence of TUNEL positive Sertoli cells significantly increased after EB (median: 2.9% vs. 0.5% for C, p<0.05 or TP+EB (median: 2.2%, p<0.01 and was not affected by other treatments. Incidence of TUNEL positive germ cells increased significantly after EB alone (median: 4.4% vs. 2.5%, for C, p<0.01 and was significantly decreased by hFSH+EB (median: 0.5%, p<0.01. CONCLUSIONS: 1 Administration of testosterone or estradiol to immature rats inhibits Sertoli cell maturation. 2 Estradiol stimulates Sertoli and germ cell apoptosis while testosterone has no effect. 3

  9. Isolated low follicle stimulating hormone (FSH in infertile males – a preliminary report

    Directory of Open Access Journals (Sweden)

    Nader Salama

    2013-09-01

    Full Text Available Objectives: High levels of follicle stimulating hormone (FSH in infertile males received a significant attention and exploration. Studies investigating the isolated deficiency of FSH in males are few, and its real prevalence is still unknown. Therefore, the objectives of the current study was to report the prevalence of isolated low FSH in infertile males and highlight their demographics and standard sperm parameters. Methods: Records of 3335 infertile men were retrospectively checked. Patients with isolated low FSH were retrieved. FSH levels were categorized into 3 groups based on the number of affected sperm parameter (s. Study variables were also arranged into 2 groups in relation to smoking history. A control group was included to document the changes in sperm morphology. Results: Isolated low FSH (1.146 ± 0.219 mIU/mL was found in 29 (0.87% patients. All patients showed at least one abnormal sperm parameter. The abnormal parameters were present in different combinations within the same patient but with no significant correlations with the FSH levels. The FSH levels got lower as the number of the affected sperm parameters increased although the decline was insignificant. The most frequent abnormal parameter presented was sperm morphology (86.2%. Anomalous sperm morphology was highly and significantly demonstrated in the head; specifically in acrosome. Abnormal sperm parameters were present in both smoking and nonsmoking groups but with no significant differences in between. Conclusions: Isolated low FSH among infertile males has a low prevalence. This may be associated with abnormality in semen parameters; particularly sperm morphology. These patients are suggested to be found as a primary entity. However, an additional work-up is highly recommended to validate this hypothesis.

  10. Application of the assay of urine FSH β-subunit in patients with pubertal disorders

    Institute of Scientific and Technical Information of China (English)

    Cui Yu-gui; Song Lin; Yang Xiao-fang; Feng Ting; Jia Yue; Pan Qin-qin; Di Fu-song; Zhou Zuo-min; Sha Jia-hao

    2004-01-01

    Objective: To evaluate the diagnostic predictive value in the identification of puberty disorders by means of ELISA of β-FSH subunit levels in successively collected urine samples compared to RIA of intact FSH in serum obtained from the normal control subjects and patients with puberty disorders, respectively.Subjects and Methods: Five male and four female volunteers were recruited as controls. Four patients with the hypogonadotropic hypogonadism, five patients with hypergonadotropic hypogonadism, four patients with the central precocious puberty and one patient with isosexual peripheral precocious puberty collected successively their early-morning urine samples for 30 to 32 days.The urine β-FSH subunit was assayed with the method of ELISA, then adjusted by creatinine (Cr) concentration.Results:Comparing with their cotemporary groups, patients with the hypogonadotropic hypogonadism had lower levels of urine β-FSH, and patients with idiopathic hypergonadism had higher levels with irregular fluctuation. Meanwhile, patients with the central precocious puberty had much higher levels of urine β-FSH with irregular peaks, and patients with isosexual peripheral precocious puberty had almost normal levels. The patterns were coincident with the clinical characteristics and serum FSH levels.Conclusion: The ELISA of urine β-FSH subunit possesses a number of advantages over the RIA of serum FSH level, such as low cost, simplicity and reliability in the clinical practice. It can be used for the diagnoses of puberty disorders. In addition, it is possible and much easier, comparing with blood samples, to collect successively urine samples for research of pathophysiological dynamics of FSH secretion in puberty disorders and other reproductive dysfunction.

  11. Effect of different FSH/LH ratios on superovulatory response and embryo yield in goats

    Directory of Open Access Journals (Sweden)

    G. Martemucci

    2011-03-01

    Full Text Available Previous studies carried out in sheep (Chupin et al., 1985 and in goats (Nowshari et al., 1995; Martemucci et al., 1996 have underlined the importance of both FSH and LH to induce a good superovulatory response, but the results on the LH amount necessary to give a higher ovulatory response and embryo production are rather contradictory. The aim of this study was to evaluate, in goats, the effect of 2 different FSH/LH ratios (1:1 vs 2:1 kept constant during the treatment, on ovulatory response and embryo production. Moreover, according to the FSH/LH ratio=2:1,.........

  12. Primary gonadal failure in men selectively amplifies the mass of follicle stimulating hormone (FSH) secreted per burst and increases the disorderliness of FSH release patterns: reversibility with testosterone replacement.

    Science.gov (United States)

    Veldhuis, J D; Iranmanesh, A; Urban, R J

    1997-10-01

    The neuroendocrine mechanisms by which primary gonadal failure in men increases mean serum FSH concentrations (castration-like response) are not known. To investigate the testosterone-dependent mechanisms of the FSH castration response: (i) blood was sampled at 10-min intervals for 24 h for later FSH assay in seven normal middle-aged men and in six patients with primary testicular failure, during testosterone withdrawal and after 6 weeks of parenteral testosterone replacement; (ii) using a specific two-site IRMA, serum FSH concentrations were measured, since this assay correlates well with an in-vitro Sertoli cell bioassay; (iii) multiparameter deconvolution analysis was then applied to estimate the frequency, amplitude, duration, and mass of underlying FSH secretory bursts, and the half-life of endogenous FSH, and (iv) approximate entropy was calculated to quantify the relative orderliness of FSH release over 24 h. Mean (+/- SEM) 24-h serum FSH concentrations were 3.9 +/- 0.8 IU/L in control subjects and 39 +/- 10 IU/L in unreplaced hypogonadal patients (p = 0.034). Deconvolution analysis revealed similar estimated mean FSH half-lives of 346 +/- 40 min (control) and 321 +/- 47 min (untreated patients), and indistinguishable FSH secretory burst frequencies, namely, 20 +/- 0.95 (normal) and 21 +/- 1.3 (patients) pulses per 24 h. In contrast, the daily production rate of FSH was markedly increased in testosterone-withdrawn hypogonadal men at 117 +/- 25 vs. 9.3 +/- 1.8 IU/L/day (control) (p gonadal failure 0.74 +/- 0.087 IU/L/min, p gonadal failure in men evokes FSH hypersecretion which is marked by more disorderly FSH release patterns and a selectively amplified mass of FSH secreted per burst. These hypergonadotrophic mechanisms are, to a significant extent, testosterone-suppressible.

  13. Effects of neonatal androgenization on the chromatofocusing pattern of anterior pituitary FSH in the female rat.

    Science.gov (United States)

    Ulloa-Aguirre, A; Damián-Matsumura, P; Espinoza, R; Dominguez, R; Morales, L; Flores, A

    1990-08-01

    Anterior pituitary glands were removed from neonatally androgenized (100 micrograms testosterone propionate) female rats and normal controls at 5, 10, 18, 21, 30, 60 and 90 days of age, and the multiple forms of FSH present within them were separated by chromatofocusing (pH range 7.5-4.0). Additional pituitary glands from intact adult males (90 days old) were also studied for comparative purposes. All animal groups exhibited multiple forms of immunoactive FSH within a pH range of 7.5-4.0, as well as an additional FSH form obtained after the addition of 1.0 mol NaCl/l to the chromatofocusing column (salt peak). In animals 5-30 days old (controls and androgenized) the majority of FSH applied to the chromatofocusing columns was recovered within the salt peak (45-85% of total FSH immunoactivity recovered). However, as the animals aged, more FSH immunoactivity focused within less acidic regions (isoelectric point (pI) 5.9-5.0); pituitaries from animals 60 days old contained the greatest proportion of FSH focused within this pH range (controls, 39.2 +/- 0.6%; androgenized, 23.1 +/- 0.9% of total immunoactivity recovered; P less than 0.03 vs animals 30 days old for both experimental groups). This shift towards less acidic FSH was attenuated in androgenized animals compared with the controls (P less than 0.01). In control adult rats, the chromatofocusing distribution pattern of pituitary FSH varied according to the day of the oestrous cycle. Pituitary extracts from control rats decapitated during the morning of pro-oestrus, oestrus and day 1 of dioestrus exhibited the highest proportion of immunoactive FSH (23.2-28.8% of total) focused within a pH range of 5.9-5.0, whilst only 10.4-11.6% of FSH from androgenized rats and those on day 1 of dioestrus was recovered within this pH range (P less than 0.05). In control animals decapitated during the morning of pro-oestrus and oestrus, 10-26% of FSH focused within the most alkaline region (pI 7.5-6.0); the chromatofocusing

  14. Genetic variations in FSH action affect sex hormone levels and breast tissue size in infant girls

    DEFF Research Database (Denmark)

    Henriksen, Louise Scheutz; Hagen, Casper P; Assens, Maria

    2016-01-01

    , especially FSHR -29G>A and FSHR 2039A>G, affect female hormone profile and glandular breast tissue development already during minipuberty. Thus, genetic variations of FSH signaling appear to determine the individual set point of the hypothalamic-pituitary-gonadal axis already early in life.......Context: Single nucleotide polymorphisms altering FSH action (FSHB -211G>T, FSHR -29G>A, and FSHR 2039A>G) are associated with peripubertal and adult levels of reproductive hormones and age at pubertal onset in girls. Objective: To investigate whether genetic polymorphisms altering FSH action...... present in homozygotes. FSHB -211T carriers had smaller breast tissue size than girls who without a minor allele; GT+TT 10.5 (confidence interval 9.4 -11.5) mm vs GG 12.1 (confidence interval 11.4-12.8) mm, P = .014. Conclusions: Our study indicates that 3 genetic polymorphisms altering FSH action...

  15. Cellular regulation of follicle-stimulating hormone (FSH) binding in rat seminiferous tubules

    Energy Technology Data Exchange (ETDEWEB)

    Kangasniemi, M.; Kaipia, A.; Toppari, J.; Perheentupa, A.; Huhtaniemi, I.; Parvinen, M. (Univ. of Turku (Finland))

    1990-07-01

    Stage-specific binding of follicle-stimulating hormone (FSH) was measured in rat seminiferous tubules. The binding in single-point assays was over 3-fold higher (P less than 0.05) in stages XIII to I than in stages VI to VII of the epithelial cycle. No difference was found between the equilibrium association constants (Ka) of FSH binding in stages XIV to IV (10 +/- 1.9 X 10(9) 1/mol) and VII to VIII (9.2 +/- 0.6 X 10(9) 1/mol, mean +/- SEM, n = 5). In another experiment, the testes were dosed locally with 3 Gy of 4 MV x-irradiation to selectively lower the number of spermatogonia. After irradiation, FSH binding in staged seminiferous tubule segments was measured when the desired types of spermatogenic cells were reduced in number. Seven days after irradiation when differentiating spermatogonia and preleptotene spermatocytes were reduced in number, FSH binding was decreased in all stages of the cycle, but the cyclic variation remained. Seventeen days after irradiation when intermediate and type B spermatogonia and spermatocytes up to diplotene of stage XIII showed low numbers, FSH binding was decreased in all stages of the cycle and the stage-dependent variation disappeared. At 38 days when pachytene spermatocytes and early spermatids were reduced in number, similar results were found. But at 52 days postirradiation when all spermatids were low in number, FSH binding was slightly elevated compared with days 17 and 38. There were no significant differences in serum FSH or LH levels between irradiated and non-irradiated animals. These findings suggest that all spermatogenic cell types may stimulate FSH binding in the Sertoli cells.

  16. Regulation of endometrial cancer cell growth by luteinizing hormone (LH) and follicle stimulating hormone (FSH)

    OpenAIRE

    Davies, S.; Bax, C M R; Chatzaki, E; Chard, Tim; Iles, Ray K.

    2000-01-01

    Gonadotrophin releasing hormone analogues (GnRHa) have been used to treat recurrent endometrial cancer. However, the mode of action is uncertain. Our previous studies showed no direct effect of GnRHa on endometrial cancer cell growth in vitro. We have now examined the effect of luteinizing hormone (LH) and follicle stimulating hormone (FSH) on endometrial cancer cell growth. The aim was to determine whether suppression of pituitary LH and FSH by GnRHa could explain the tumour regression seen ...

  17. Inter-nesting habitat-use patterns of loggerhead sea turtles: Enhancing satellite tracking with benthic mapping

    Science.gov (United States)

    Hart, Kristen M.; Zawada, David G.; Fujisaki, Ikuko; Lidz, Barbara H.

    2010-01-01

    The loggerhead sea turtle Caretta caretta faces declining nest numbers and bycatches from commercial longline fishing in the southeastern USA. Understanding spatial and temporal habitat-use patterns of these turtles, especially reproductive females in the neritic zone, is critical for guiding management decisions. To assess marine turtle habitat use within the Dry Tortugas National Park (DRTO), we used satellite telemetry to identify core-use areas for 7 loggerhead females inter-nesting and tracked in 2008 and 2009. This effort represents the first tracking of DRTO loggerheads, a distinct subpopulation that is 1 of 7 recently proposed for upgrading from threatened to endangered under the US Endangered Species Act. We also used a rapid, high-resolution, digital imaging system to map benthic habitats in turtle core-use areas (i.e. 50% kernel density zones). Loggerhead females were seasonal residents of DRTO for 19 to 51 d, and individual inter-nesting habitats were located within 1.9 km (2008) and 2.3 km (2009) of the nesting beach and tagging site. The core area common to all tagged turtles was 4.2 km2 in size and spanned a depth range of 7.6 to 11.5 m. Mapping results revealed the diversity and distributions of benthic cover available in the core-use area, as well as a heavily used corridor to/from the nesting beach. This combined tagging-mapping approach shows potential for planning and improving the effectiveness of marine protected areas and for developing spatially explicit conservation plans.

  18. Enhanced drought-tolerance in the homoploid hybrid species Pinus densata: implication for its habitat divergence from two progenitors.

    Science.gov (United States)

    Ma, Fei; Zhao, Changming; Milne, Richard; Ji, Mingfei; Chen, Litong; Liu, Jianquan

    2010-01-01

    The homoploid hybrid species Pinus densata is restricted to alpine habitats that exceed the altitude range of its two parental species, Pinus tabulaeformis and Pinus yunnanensis. Alpine habitats usually generate cold-induced water stress in plants. To understand the ecological differentiation between these three species, we examined their physiological responses to drought stress. Potted seedlings of three species were subjected to low, mild, moderate and severe water stress in an automatic-controlled glasshouse. Fifteen indicators of fitness were measured for each species in each treatment, and most of these decreased as drought increased. Pinus densata exhibited higher fitness than both parental species in terms of total dry mass production (TDM) and long-term water use efficiency (WUE(L)) across all treatments; several other ecophysiological traits were also extreme but not across every treatment, and not always in the highest stress treatment. These results indicate that extreme characters that have become well fixed in P. densata, confer a faster seedling growth rate and more efficient water use, which in turn should confer increased drought tolerance. These traits of P. densata likely promoted its ecological separation from its parental species and facilitated its successful colonization and establishment in high-altitude habitats.

  19. Influence of corticosterone on FSH-induced ovarian recrudescence in the lizard Mabuya carinata.

    Science.gov (United States)

    Nijagal, B S; Yajurvedi, H N

    1999-09-01

    Administration of bovine FSH (10 IU/lizard/alternate day for 30 days) in the postbreeding quiescent phase of the ovarian cycle caused a significant increase in the mean number of oogonia and oocytes, the relative weight of the oviduct, and the liver and serum estradiol levels compared to those of controls. In addition, the FSH-treated lizards showed a vitellogenic growth of follicles and development through to preovulatory follicles. However, the administration of corticosterone simultaneously with FSH (10 IU FSH + 40 microgram corticosterone/lizard/alternate day for 30 days) did not result in these changes and the ovaries resembled those of controls. The results indicate the absence of ovarian refractoriness to gonadotropic stimulation during the quiescent phase of the reproductive cycle and inhibition of FSH-induced ovarian recrudescence by corticosterone. It is suggested that corticosterone treatment reduces FSH-induced steroidogenic activity of the ovary, leads to impairment in vitellogenin secretion by the liver, and results as well in the failure of vitellogenic follicular growth in Mabuya carinata.

  20. Corticosterone inhibits normal and FSH-induced testicular recrudescence in the lizard, Mabuya carinata.

    Science.gov (United States)

    Yajurvedi, H N; Nijagal, B S

    2000-12-01

    Administration (ip) of 1, 10, or 20 microg corticosterone (alternate days for 30 days) to adult male Mabuya carinata did not affect the seasonal recrudescence of spermatogenesis whereas administration of 40 microg corticosterone did result in inhibition of spermatogenesis. Further, administration of FSH (10 IU/lizard/alternate day for 30 days) during the quiescent phase of the testicular cycle stimulated spermatogenetic and steroidogenic activity of the testis as shown by significant increases in the mean number of spermatogonia, spermatocytes, and spermatids and serum levels of testosterone. In addition there were abundant spermatozoa in the lumen of the tubules in FSH-treated lizards. Administration of 10 IU FSH + 40 microg corticosterone (per lizard on alternate days for 30 days) increased the mean number of primary and secondary spermatocytes whereas the mean number of spermatids did not show significant variation compared with that of controls. Further, the mean numbers of spermatocytes and spermatids and serum levels of testosterone were significantly less when compared to those of FSH alone treated lizards. In addition, FSH-induced development of epididymis was also inhibited by corticosterone treatment. The results indicate that corticosterone inhibits FSH-induced testicular recrudescence, possibly by suppressing testosterone secretion in M. carinata.

  1. Induksi Superovulasi dengan Kombinasi CIDR, Hormon FSH dan hCG pada Induk Sapi Potong

    Directory of Open Access Journals (Sweden)

    E.M. Kaiin

    2006-12-01

    Full Text Available The aim of this study was to evaluate the effect of superovulation treatment using combination of CIDR, FSH and hCG in beef cattle as donor embryos using MOET programme. All animals had been palpated to evaluated the ovary status and normal cows were used as donor and synchronized with CIDR (Eazy BreedTM . At day 10 of oestrus cycle, cows were divided into two groups, first group: cows were injected intramuscularly with FSH (Antrin 40 IU per cow with decreasing doses (for 4 days and second group cows were treated the same way but at day 5 after FSH injection, they were injected intramuscularly with 1,500 IU hCG (Chorulon. Embryo collection was done at day 7 after Artificial Insemination (AI. Average number of corpora lutea (CL in animals that superovulated with CIDR, FSH and hCG was significantly higher (P<0.05 compared to animals treated with CIDR and FSH only (5.52. Average number of embryo collection and number of transferable embryos were also higher in group treated with hCG (6.00 vs 5.44 compared with those treated without hCG (4.33 vs 3.17. The conclusion is hCG superovulation injection with CIDR and FSH can increase the respon of superovulation

  2. Channel Morphology and Bed Sediment Characteristics Before and After Habitat Enhancement Activities in the Uridil Property, Platte River, Nebraska, Water Years 2005-2008

    Science.gov (United States)

    Kinzel, Paul J.

    2009-01-01

    Fluvial geomorphic data were collected by the United States Geological Survey from July 2005 to June 2008 (a time period within water years 2005 to 2008) to monitor the effects of habitat enhancement activities conducted in the Platte River Whooping Crane Maintenance Trust's Uridil Property, located along the Platte River, Nebraska. The activities involved the removal of vegetation and sand from the tops of high permanent islands and the placement of the sand into the active river channel. This strategy was intended to enhance habitat for migratory water birds by lowering the elevations of the high islands, thereby eliminating a visual obstruction for roosting birds. It was also thought that the bare sand on the lowered island surfaces could serve as potential habitat for nesting water birds. Lastly, the project supplied a local source of sediment to the river to test the hypothesis that this material could contribute to the formation of lower sandbars and potential nesting sites downstream. Topographic surveys on the islands and along river transects were used to quantify the volume of removed sand and track the storage and movement of the introduced sand downstream. Sediment samples were also collected to map the spatial distribution of river bed sediment sizes before and after the management activities. While the project lowered the elevation of high islands, observations of the sand addition indicated the relatively fine-grained sand that was placed in the active river channel was rapidly transported by the flowing water. Topographic measurements made 3 months after the sand addition along transects in the area of sediment addition showed net aggradation over measurements made in 2005. In the year following the sand addition, 2007, elevated river flows from local rain events generally were accompanied by net degradation along transects within the area of sediment addition. In the spring of 2008, a large magnitude flow event of approximately 360 cubic meters per

  3. Theca cells and theca-cell conditioned medium inhibit the progression of FSH-induced meiosis of bovine oocytes surrounded by cumulus cells connected to membrana granulosa.

    Science.gov (United States)

    van Tol, H T; Bevers, M M

    1998-11-01

    progressed up to the MII stage was significantly reduced. Attachment of the COCs to the membrana granulosa enhanced this inhibiting action of CMt on the progression of meiosis. It is concluded that theca cells secrete a stable factor that inhibits the progression of FSH-mediated meiosis in oocytes of COCGs.

  4. Regulation and regulatory role of WNT signaling in potentiating FSH action during bovine dominant follicle selection.

    Directory of Open Access Journals (Sweden)

    P S P Gupta

    Full Text Available Follicular development occurs in wave like patterns in monotocous species such as cattle and humans and is regulated by a complex interaction of gonadotropins with local intrafollicular regulatory molecules. To further elucidate potential mechanisms controlling dominant follicle selection, granulosa cell RNA harvested from F1 (largest and F2 (second largest follicles isolated at predeviation (PD and onset of diameter deviation (OD stages of the first follicular wave was subjected to preliminary RNA transcriptome analysis. Expression of numerous WNT system components was observed. Hence experiments were performed to test the hypothesis that WNT signaling modulates FSH action on granulosa cells during follicular waves. Abundance of mRNA for WNT pathway members was evaluated in granulosa cells harvested from follicles at emergence (EM, PD, OD and early dominance (ED stages of the first follicular wave. In F1 follicles, abundance of CTNNB1 and DVL1 mRNAs was higher and AXIN2 mRNA was lower at ED versus EM stages and DVL1 and FZD6 mRNAs were higher and AXIN2 mRNA was lower in F1 versus F2 follicle at the ED stage. Bovine granulosa cells were treated in vitro with increasing doses of the WNT inhibitor IWR-1+/- maximal stimulatory dose of FSH. IWR-1 treatment blocked the FSH-induced increase in granulosa cell numbers and reduced the FSH-induced increase in estradiol. Granulosa cells were also cultured in the presence or absence of FSH +/- IWR-1 and hormonal regulation of mRNA for WNT pathway members and known FSH targets determined. FSH treatment increased CYP19A1, CCND2, CTNNB1, AXIN2 and FZD6 mRNAs and the stimulatory effect on CYP19A1 mRNA was reduced by IWR-1. In contrast, FSH reduced CARTPT mRNA and IWR-1 partially reversed the inhibitory effect of FSH. Results support temporal and hormonal regulation and a potential role for WNT signaling in potentiating FSH action during dominant follicle selection.

  5. 200 THE USE OF LONG-ACTING FSH-MAP5 IN SHEEP SUPEROVULATION PROGRAMS.

    Science.gov (United States)

    Fry, R

    2016-01-01

    The administration of sustained-release FSH-MAP5 in a 2-injection protocol has been shown to be as effective as a multiple-FSH injection protocol in inducing superovulation in cattle (Bo and Mapletoft 2014 Theriogenology 81, 38) and in sheep (Fry et al. 2016 Reprod. Fertil. Dev. 28, 250); however, the effect on embryo quality in the latter experiment was unclear. The following experiment further investigated the effect of FSH-MAP5 on ovulation rate and embryo quality in a sheep multiple-ovulation embryo transfer (MOET) program conducted in the breeding season. Two hundred sixteen Dohne merino ewes received a 12-day CIDR-S device containing 0.33g of progesterone (Zoetis, Florham Park, NJ, USA) plus 200mg of FSH IM (Folltropin-V; Vetoquinol, Belleville, Canada) and 400IU of eCG IM (Pregnecol: Vetoquinol) in 4 treatment groups. Group 1 (n=51) received 7 injections (a.m., p.m.) of FSH in saline (2.5, 2.0, 1.5, 1.5, 1.0, 1.0, and 0.5mL) starting 2.5 days before CIDR withdrawal and 400IU of eCG in saline at the time of the first FSH injection. Group 2 (n=53) received 6 injections (a.m., p.m.) of FSH in saline (3.0, 2.0, 1.5, 1.5, 1.0, and 1.0mL) starting 2.5 days before CIDR withdrawal and 400IU of eCG in saline at CIDR withdrawal. Group 3 (n=56) received 3.3mL of FSH in hyaluronan (50mg of MAP5, Vetoquinol) and 400IU of eCG in saline 2.5 days before CIDR withdrawal and 1.7mL of FSH-MAP5 at 0.5 days before CIDR withdrawal. Group 4 (n=56) received 3.3mL of FSH-MAP5 at 2.5 days before CIDR withdrawal, 1.7mL of FSH-MAP5 at 0.5 days before CIDR withdrawal, and 400IU of eCG in saline at CIDR withdrawal. Ewes were inseminated with semen collected and pooled from 5 rams at 36 to 40h after CIDR withdrawal. Donor ewes were slaughtered 6 days after AI, corpora lutea were counted on the ovary, and ova/embryos were collected. Data for corpora lutea, total ova/embryo, and transferable embryo was analysed by the Kruskal-Wallis test and differences between groups were determined by the

  6. Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation.

    Science.gov (United States)

    Chowdhury, Indrajit; Thomas, Kelwyn; Zeleznik, Anthony; Thompson, Winston E

    2016-05-01

    Published results from our laboratory identified prohibitin (PHB), a gene product expressed in granulosa cells (GCs) that progressively increases during follicle maturation. Our current in vitro studies demonstrate that follicle-stimulating hormone (FSH) stimulates Phb expression in rat primary GCs. The FSH-dependent expression of PHB was primarily localized within mitochondria, and positively correlates with the morphological changes in GCs organelles, and synthesis and secretions of estradiol (E2) and progesterone (P4). In order to confirm that PHB plays a regulatory role in rat GC differentiation, endogenous PHB-knockdown studies were carried out in undifferentiated GCs using adenoviral (Ad)-mediated RNA interference methodology. Knockdown of PHB in GCs resulted in the suppression of the key steroidogenic enzymes including steroidogenic acute regulatory protein (StAR), p450 cholesterol side-chain cleavage enzyme (p450scc), 3β-hydroxysteroid dehydrogenase (3β-HSD), and aromatase (Cyp19a1); and decreased E2 and P4 synthesis and secretions in the presence of FSH stimulation. Furthermore, these experimental studies also provided direct evidence that PHB within the mitochondrial fraction in GCs is phosphorylated at residues Y249, T258, and Y259 in response to FSH stimulation. The observed levels of phosphorylation of PHB at Y249, T258, and Y259 were significantly low in GCs in the absence of FSH stimulation. In addition, during GC differentiation FSH-induced expression of phospho-PHB (pPHB) requires the activation of MEK1-ERK1/2 signaling pathway. Taken together, these studies provide new evidence supporting FSH-dependent PHB/pPHB upregulation in GCs is required to sustain the differentiated state of GCs.

  7. The Immunoexpression of FSH-R in the Ductuli Efferentes and the Epididymis of Men and Rat: Effect of FSH on the Morphology and Steroidogenic Activity of Rat Epididymal Epithelial Cells In Vitro

    Directory of Open Access Journals (Sweden)

    Małgorzata Świder-Al-Amawi

    2010-01-01

    Full Text Available The Sertoli cells were regarded as the only target for FSH in male reproductive system. The expression of FSH receptor (FSH-R was detected also in epithelial cells of the caput epididymis of rat and monkey. We showed in the immunohistochemistry study the expression of FSH-R in rat and human ductuli efferentes and the caput, corpus, and cauda epididymis, moreover, by Western blot analysis in the caput and cauda epididymis of rat. Additionally, we presented that the morphology of rat epididymal epithelial cells in vitro was affected by FSH, and FSH stimulation resulted in the increase of 17β-estradiol synthesis by rat caput epididymal cells in dose-depended manner. In conclusion, the identification of FSH receptors in human and rat epididymides supports our results that the epididymis is a target organ not only for LH but additionally for FSH. On the basis of the results we showed for the first time that morphology of epididymal epithelial cells and epididymal steroidogenesis can be regulated by FSH.

  8. Effects of estradiol and FSH on maturation of the testis in the hypogonadal (hpg mouse

    Directory of Open Access Journals (Sweden)

    Mayhew Terry M

    2008-01-01

    Full Text Available Abstract Background The hypogonadal (hpg mouse is widely used as an animal model with which to investigate the endocrine regulation of spermatogenesis. Chronic treatment of these GnRH-deficient mice with estradiol is known to induce testicular maturation and restore qualitatively normal spermatogenesis. The aim of the current studies was to investigate whether these effects of estradiol are direct effects in the testis, or indirect actions via paradoxical stimulation of FSH secretion from the pituitary gland. Methods Initially, Western blot and immunohistochemistry were used to analyse tissues from hpg mice to identify potential sites of action of estradiol. In the main study, hpg mice were treated for 50 days with either an estradiol implant or daily injections of recombinant human FSH, or a combination of both, to determine whether estradiol would have an additive or synergistic effect with FSH on testis development, as assessed by histological analysis and stereological quantification of Leydig, Sertoli and germ cell proliferation. Results Western blot analysis revealed ERα immunoreactive bands of appropriate molecular weight in extracts of testis and pituitary glands from hpg mice, and immunohistochemical studies confirmed ERα in nuclei of anterior pituitary cells and Leydig and peritubular cells in hpg mice. Histological and morphometric analyses revealed that estradiol treatment alone was as effective as FSH in promoting Sertoli cell production and proliferation of the seminiferous epithelium, resulting in the production of elongating spermatids. Combined estradiol and FSH treatment did not produce a greater effect than either treatment alone, though an increased dose of FSH significantly increased seminiferous tubule volume and testis weight and increase Sertoli cell numbers further within the same time frame. In contrast, estradiol caused substantial increases in the wet weight of the seminal vesicles, whereas FSH was without effect on

  9. Estuarine and Tidal Freshwater Habitat Cover Types Along the Lower Columbia River Estuary Determined from Landsat 7 Enhanced Thematic Mapper (ETM+) Imagery, Technical Report 2003.

    Energy Technology Data Exchange (ETDEWEB)

    Garono, Ralph; Robinson, Rob

    2003-10-01

    Developing an understanding of the distribution and changes in estuarine and tidal floodplain ecosystems is critical to the management of biological resources in the lower Columbia River. Columbia River plants, fish, and wildlife require specific physicochemical and ecological conditions to sustain their populations. As habitats are degraded or lost, this capability is altered, often irretrievably; those species that cannot adapt are lost from the ecosystem. The Lower Columbia River Estuary Partnership (Estuary Partnership) completed a comprehensive ecosystem protection and enhancement plan for the lower Columbia River and estuary in 1999 (Jerrick, 1999). The plan identified habitat loss and modification as a critical threat to the integrity of the lower Columbia River ecosystem and called for a habitat inventory as a key first step in its long term restoration efforts. In 2000, the Estuary Partnership initiated a multiphase project to produce a spatial data set describing the current location and distribution of estuarine and tidal freshwater habitat cover types along the lower Columbia River from the river mouth to the Bonneville Dam using a consistent methodology and data sources (Fig. 1). The first phase of the project was the development of a broadbrush description of the estuarine and tidal freshwater habitat cover classes for the entire study area ({approx}146 river miles) using Landsat 7 ETM+ satellite imagery. Phase II of the project entailed analysis of the classified satellite imagery from Phase I. Analysis of change in landcover and a summary of the spatial relationships between cover types are part of Phase II. Phase III of the project included the classification of the high resolution hyperspectral imagery collected in 2000 and 2001 for key focal areas within the larger study area. Finally, Phase IV consists of this final report that presents results from refining the Landsat ETM+ classification and provides recommendations for future actions

  10. Effects of photoperiod and hypothalamic knife cuts on the timing of FSH surges in hamsters.

    Science.gov (United States)

    Badura, L L; Sisk, C L; Nunez, A A

    1991-02-01

    The timing of the proestrous surge of follicle-stimulating hormone (FSH) was examined in female hamsters with hypothalamic knife cuts that prevented reproductive responses to photoperiod. All animals received either a horizontal knife cut aimed between the suprachiasmatic nuclei (SCN) and the paraventricular nuclei (PVN), or sham surgery, and were housed in long (16 h of light/24 h) or short (6 h of light/24 h) photoperiods. Following exposure to either photo-period for 11-12 weeks, a subset of the animals was fitted with an indwelling jugular cannula. Blood samples were taken hourly over a 24-h period and plasma levels of FSH were determined by RIA. Knife cuts placed ventral to or through the ventral portions of the PVN prevented short day-induced anestrus. On the day of proestrus, peak elevations of FSH in cycling animals with knife cuts in both photoperiods, as well as in sham-operated females in long days, occurred 4-5 h before lights out. In contrast, sham-operated anestrous females in short days showed peak elevations of FSH approximately 3-4 h after lights out. The present results support the view that neural connections between the SCN and the PVN mediate the effects of short days on reproductive physiology, including changes in the timing of the FSH surge.

  11. Hypersecretion of FSH in infant boys and girls born small for gestational age.

    Science.gov (United States)

    Ibáñez, Lourdes; Valls, Carme; Cols, Maria; Ferrer, Angela; Marcos, Maria Victoria; De Zegher, Francis

    2002-05-01

    Prenatal growth restraint, as reflected in a low birthweight for gestational age, is a risk factor for postpubertal FSH hypersecretion and for reduced gonadal size. The ontogeny of the low-birthweight effect on the FSH-inhibin B feedback loop is unknown. Infancy is an episode of choice to study the possibility of an early low-birthweight effect on the FSH-inhibin B loop because this phase is characterized by high activity within the gonadal axis. We assessed serum concentrations of FSH and inhibin B in 46 infants [26 girls and 20 boys; mean age, 4 months; range, 3-6 months; 17 appropriate for gestational age (AGA), 29 small for gestational age (SGA); mean birthweight, 3.2 kg for AGA vs. 2.3 kg for SGA], together with circulating levels of LH, E2, and free androgen index. In SGA girls and boys, serum FSH levels were 2- and 4-fold higher (P hypersecretion in infancy is a marker of subsequent subfertility.

  12. Addition of low dose hCG to rFSh benefits older women during ovarian stimulation for IVF

    Directory of Open Access Journals (Sweden)

    Gomaa Hala

    2012-08-01

    Full Text Available Abstract Background To compare the outcome of IVF cycles in women receiving controlled ovarian stimulation with recFSH or recFSH plus low dose hCG. Methods A retrospective case control study, performed at a private practice affiliated with an academic institute. Patients were infertile women who were treated with IVF/ICSI and controlled ovarian stimulation in a long GnRH agonist protocol using either low dose hCG in addition to recFSH [N = 88] or recFSH alone [N = 99]. Primary outcomes were mean FSH dose, number of mature eggs, number of fertilized eggs, and serum levels of estradiol. Secondary outcomes were endometrial thickness, cycle cancellations and pregnancy rates. Results A significant increase in number of mature and fertilized eggs was observed in women over 40 years of age using low dose hCG in addition to recFSH. The estradiol level was significantly higher on the day of hCG administration and the serum level of FSH on cycle day 7 and on the day of hCG administration were lower. Conclusion Addition of low dose hCG to recFSH compared with recFSH alone significantly modified cycle characteristics in patients >/= 40 years and could be of potential benefit for IVF cycles in older infertile women.

  13. At what spatial scale do high-quality habitats enhance the diversity of forbs and pollinators in intensively farmed landscapes?

    NARCIS (Netherlands)

    Kohler, F.; Verhulst, J.; Klink, van R.; Kleijn, D.

    2008-01-01

    1. Over the last decades, biodiversity in agricultural landscapes has declined drastically. Initiatives to enhance biodiversity, such as agri-environment schemes, often have little effect, especially in intensively farmed landscapes. The effectiveness of conservation management may be improved by

  14. Pubertal Onset in Girls is Strongly Influenced by Genetic Variation Affecting FSH Action

    Science.gov (United States)

    Hagen, Casper P.; Sørensen, Kaspar; Aksglaede, Lise; Mouritsen, Annette; Mieritz, Mikkel G.; Tinggaard, Jeanette; Wohlfart-Veje, Christine; Petersen, Jørgen Holm; Main, Katharina M.; Meyts, Ewa Rajpert-De; Almstrup, Kristian; Juul, Anders

    2014-01-01

    Age at pubertal onset varies substantially in healthy girls. Although genetic factors are responsible for more than half of the phenotypic variation, only a small part has been attributed to specific genetic polymorphisms identified so far. Follicle-stimulating hormone (FSH) stimulates ovarian follicle maturation and estradiol synthesis which is responsible for breast development. We assessed the effect of three polymorphisms influencing FSH action on age at breast deveopment in a population-based cohort of 964 healthy girls. Girls homozygous for FSHR -29AA (reduced FSH receptor expression) entered puberty 7.4 (2.5–12.4) months later than carriers of the common variants FSHR -29GG+GA, p = 0.003. To our knowledge, this is the strongest genetic effect on age at pubertal onset in girls published to date. PMID:25231187

  15. Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH

    Directory of Open Access Journals (Sweden)

    Evelyn Rabelo Andrade

    2011-01-01

    Full Text Available The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA, Epidermal Growth Factor (EGF, and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6 d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

  16. Superovulation of goats with purified pFSH supplemented with defined amounts of pLH.

    Science.gov (United States)

    Nowshari, M A; Backers, J F; Holtz, W

    1995-03-01

    The superovulatory response of goats treated with purified pFSH supplemented with 30, 40 or 50% pLH was compared. Sixty-four Boer goat does were synchronized by progestagen-containing ear implant, randomly allotted to 3 groups and, beginning 2 d before implant removal, treated with purified pFSH supplemented with 30, 40 or 50% pLH. Each animal received 16 Armour Units of pFSH administered in 6 descending doses at 12-h intervals. Along with the last 2 injections, the does received 5 mg PGF(2alpha). Embryos were flushed either surgically or after slaughter on Day 5 or 6 after the last day of standing estrus. The percentage of animals responding to treatment was not different among groups treated with pFSH supplemented with 30, 40 or 50% pLH (76, 71 and 63%, respectively). The corresponding data for number of ovulations was 11.3 +/- 1.6, 16.3 +/- 1.8 and 16.4 +/- 2.6, for number of ova and embryos recovered 8.1 +/- 1.9, 12.0 +/- 1.5 and 13.5 +/- 2.9 and for number of transferable embryos 6.6 +/- 1.9, 9.1 +/- 1.5 and 7.1 +/- 2.1 (x +/- SEM). Results confirm the earlier finding of a good response of goats to pFSH preparations with a high FSH:LH ratio, and, although group differences were statistically nonsignificant (P > 0.05), they suggest that supplementation with approximately 40% pLH may be close to the optimum.

  17. Fsh and Lh direct conserved and specific pathways during flatfish semicystic spermatogenesis.

    Science.gov (United States)

    Chauvigné, François; Zapater, Cinta; Crespo, Diego; Planas, Josep V; Cerdà, Joan

    2014-10-01

    The current view of the control of spermatogenesis by Fsh and Lh in non-mammalian vertebrates is largely based on studies carried out in teleosts with cystic and cyclic spermatogenesis. Much less is known concerning the specific actions of gonadotropins during semicystic germ cell development, a type of spermatogenesis in which germ cells are released into the tubular lumen where they transform into spermatozoa. In this study, using homologous gonadotropins and a candidate gene approach, for which the genes' testicular cell-type-specific expression was established, we investigated the regulatory effects of Fsh and Lh on gene expression during spermatogenesis in Senegalese sole (Solea senegalensis), a flatfish with asynchronous and semicystic germ cell development. During early spermatogenesis, Fsh and Lh upregulated steroidogenesis-related genes and nuclear steroid receptors, expressed in both somatic and germ cells, through steroid-dependent pathways, although Lh preferentially stimulated the expression of downstream genes involved in androgen and progestin syntheses. In addition, Lh specifically promoted the expression of spermatid-specific genes encoding spermatozoan flagellar proteins through direct interaction with the Lh receptor in these cells. Interestingly, at this spermatogenic stage, Fsh primarily regulated genes encoding Sertoli cell growth factors with potentially antagonistic effects on germ cell proliferation and differentiation through steroid mediation. During late spermatogenesis, fewer genes were regulated by Fsh or Lh, which was associated with a translational and posttranslational downregulation of the Fsh receptor in different testicular compartments. These results reveal that conserved and specialized gonadotropic pathways regulate semicystic spermatogenesis in flatfish, which may spatially adjust cell germ development to maintain a continuous reservoir of spermatids in the testis.

  18. Effects of Ascorbic Acid and FSH on the Maturation of Mice's Oocytes and Follicles

    Directory of Open Access Journals (Sweden)

    Barzegari Firouzabadi

    2011-11-01

    Full Text Available Introduction: Progress in laboratory culture conditions for in vitro oocyte maturation has led to development of the treatment of human and animal infertility. In this study we investigated the effects of FSH and ascorbic acid on the in vitro maturation of mouse's follicles and enclosed oocytes. Methods: For experiment, intact pre-antral follicles were isolated from the ovaries of 6 week-old female mice and cultured in TCM-199 medium. Special quantities of FSH and ascorbic acid were added to the culture medium (containing 25-30 follicles during separate experiments: 5, 20, 40, 60, 100, 140, 180 and 220 IU/L of FSH and 20, 40, 80, 240, 300 and 400 nmol/mL of ascorbic acid. Follicles were cultured for 6 days in an incubator at 37 °C, 92% humidity and 5% air CO2. Our study was semi-experimental. The entire statistical analysis was carried out by SPSS (version 14.0 for Windows using one way ANOVA. Post Hoc tests were used for the multiple comparisons at 95% confidence interval. P values < 0.05 were considered statistically significant. Results: At FSH concentration of 100 IU/L increase in follicle diameter (190µm, survival rate (91%, germinal vesicle breakdown (GVBD (81% and oocyte maturation rates (61% was observed (p ≥0.05. Ascorbic acid increased survival rate (42%, p<0.001 but didn’t affect diameter, GVBD and oocyte maturation rates. Conclusion: Ascorbic acid and FSH-containing medium showed a marked increase in all parameters except for follicle diameter. FSH and ascorbic acid increase the maturation rate of follicles and enclosed oocytes but if they are supplied in a combination, this growth rate can be significantly increased

  19. An FSH and TSH pituitary adenoma, presenting with precocious puberty and central hyperthyroidism.

    Science.gov (United States)

    Vargas, Guadalupe; Balcazar-Hernandez, Lourdes-Josefina; Melgar, Virgilio; Magriña-Mercado, Roser-Montserrat; Gonzalez, Baldomero; Baquera, Javier; Mercado, Moisés

    2017-01-01

    A 19-year-old woman with a history of isosexual precocious puberty and bilateral oophorectomy at age 10 years because of giant ovarian cysts, presents with headaches and mild symptoms and signs of hyperthyroidism. Hormonal evaluation revealed elevated FSH and LH levels in the postmenopausal range and free hyperthyroxinemia with an inappropriately normal TSH. Pituitary MRI showed a 2-cm macroadenoma with suprasellar extension. She underwent successful surgical resection of the pituitary tumor, which proved to be composed of two distinct populations of cells, each of them strongly immunoreactive for FSH and TSH, respectively. This mixed adenoma resulted in two different hormonal hypersecretion syndromes: the first one during childhood and consisting of central precocious puberty and ovarian hyperstimulation due to the excessive secretion of biologically active FSH and which was not investigated in detail and 10 years later, central hyperthyroidism due to inappropriate secretion of biologically active TSH. Although infrequent, two cases of isosexual central precocious puberty in girls due to biologically active FSH secreted by a pituitary adenoma have been previously reported in the literature. However, this is the first reported case of a mixed adenoma capable of secreting both, biologically active FSH and TSH. Although functioning gonadotrophinomas are infrequent, they should be included in the differential diagnosis of isosexual central precocious puberty.Some functioning gonadotrophinomas are mixed adenomas, secreting other biologically active hormones besides FSH, such as TSH.Early recognition and appropriate treatment of these tumors by transsphenoidal surgery is crucial in order to avoid unnecessary therapeutic interventions that may irreversibly compromise gonadal function.

  20. Habitat Selection

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — These data provide information on the relationship between California red-legged frogs and their habitat in a unique ecosystem to better conserve this threatened...

  1. Habitat Observations

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — These data provide information on the relationship between California red-legged frogs and their habitat in a unique ecosystem to better conserve this threatened...

  2. FSH and TSH in the Regulation of Bone Mass: The Pituitary/Immune/Bone Axis

    Directory of Open Access Journals (Sweden)

    Graziana Colaianni

    2013-01-01

    Full Text Available Recent evidences have highlighted that the pituitary hormones have profound effects on bone, so that the pituitary-bone axis is now becoming an important issue in the skeletal biology. Here, we discuss the topical evidence about the dysfunction of the pituitary-bone axis that leads to osteoporotic bone loss. We will explore the context of FSH and TSH hormones arguing their direct or indirect role in bone loss. In addition, we will focus on the knowledge that both FSH and TSH have influence on proinflammatory and proosteoclastogenic cytokine expression, such as TNFα and IL-1, underlining the correlation of pituitary-bone axis to the immune system.

  3. Validation of a noninvasive diagnostic tool to verify neuter status in dogs: The urinary FSH to creatinine ratio.

    Science.gov (United States)

    Albers-Wolthers, C H J; de Gier, J; Oei, C H Y; Schaefers-Okkens, A C; Kooistra, H S

    2016-09-15

    Determining the presence of functional gonadal tissue in dogs can be challenging, especially in bitches during anestrus or not known to have been ovariectomized, or in male dogs with nonscrotal testes. Furthermore, in male dogs treated with deslorelin, a slow-release GnRH agonist implant for reversible chemical castration, the verification of complete downregulation of the hypothalamic-pituitary-gonadal (HPG) axis can be difficult, especially if pretreatment parameters such as the size of the testes or prostate gland are not available. The aims of this study were to validate an immunoradiometric assay for measurement of FSH in canine urine, to determine if the urinary FSH to creatinine ratio can be used to verify the neuter status in bitches and male dogs, as an alternative to the plasma FSH concentration, and to determine if downregulation of the HPG axis is achieved in male dogs during deslorelin treatment. Recovery of added canine FSH and serial dilutions of urine reported that the immunoradiometric assay measures urinary FSH concentration accurately and with high precision. Plasma FSH concentrations (the mean of two samples, taken 40 minutes apart) and the urinary FSH to creatinine ratio were determined before gonadectomy and 140 days (median, range 121-225 days) and 206 days (median, range 158-294 days) after gonadectomy of 13 bitches and five male dogs, respectively, and in 13 male dogs before and 132 days (median, range 117-174 days) after administration of a deslorelin implant. In both bitches and male dogs, the plasma FSH concentration and the urinary FSH to creatinine ratio were significantly higher after gonadectomy, with no overlapping of their ranges. Receiver operating characteristic analysis of the urinary FSH to creatinine ratio revealed a cut-off value of 2.9 in bitches and 6.5 in males to verify the presence or absence of functional gonadal tissue. In male dogs treated with deslorelin, the plasma FSH concentrations and urinary FSH to

  4. The relation of germ cells per tubule in testes, serum inhibin B and FSH in cryptorchid boys

    DEFF Research Database (Denmark)

    Cortes, D; Thorup, Jørgen Mogens; Hogdall, E

    2007-01-01

    and FSH measurements with testicular biopsy parameters in bilateral cryptorchid boys. Included were 25 boys with bilateral cryptorchidism, median 2.5 years (9 months to 5.5 years) at surgery for bilateral cryptorchidism with simultaneous testicular biopsies, and blood sample for inhibin B and FSH...

  5. FSH inhibits the augmentation by oestradiol of the pituitary responsiveness to GnRH in the female rat

    NARCIS (Netherlands)

    Schuiling, GA; Valkhof, N; Koiter, TR

    1999-01-01

    The effect of follicle stimulating hormone (FSH) treatment on the pituitary response to gonadotrophin-releasing hormone (GnRH) was studied in rats in various reproductive conditions. A 3-day treatment of cycling rats with FSH (Metrodin(R); 10 IU/injection) lowered the spontaneous pre-ovulatory LH-su

  6. The OPTIMIST study: optimisation of cost effectiveness through individualised FSH stimulation dosages for IVF treatment. A randomised controlled trial

    NARCIS (Netherlands)

    Tilborg, T.C. van; Eijkemans, M.J.; Laven, J.S.E.; Koks, C.A.; Bruin, J.P. de; Scheffer, G.J.; Golde, R.J. van; Fleischer, K.; Hoek, A.; Nap, A.W.; Kuchenbecker, W.; Manger, P.A.; Brinkhuis, E.A.; Heusden, A.M. van; Sluijmer, A.V.; Verhoeff, A.; Hooff, M.H. van; Friederich, J.; Smeenk, J.M.J.; Kwee, J.; Verhoeve, H.R.; Lambalk, C.B.; Helmerhorst, F.M.; Veen, F. van der; Mol, B.W.; Torrance, H.L.; Broekmans, F.J.

    2012-01-01

    ABSTRACT: BACKGROUND: Costs of in vitro fertilisation (IVF) are high, which is partly due to the use of follicle stimulating hormone (FSH). FSH is usually administered in a standard dose. However, due to differences in ovarian reserve between women, ovarian response also differs with potential negat

  7. The OPTIMIST study : optimisation of cost effectiveness through individualised FSH stimulation dosages for IVF treatment. A randomised controlled trial

    NARCIS (Netherlands)

    van Tilborg, Theodora C.; Eijkemans, Marinus J. C.; Laven, Joop S. E.; Koks, Carolien A. M.; de Bruin, Jan Peter; Scheffer, Gabrielle J.; van Golde, Ron J. T.; Fleischer, Kathrin; Hoek, Annemieke; Nap, Annemiek W.; Kuchenbecker, Walter K. H.; Manger, Petra A.; Brinkhuis, Egbert A.; van Heusden, Arne M.; Sluijmer, Alexander V.; Verhoeff, Arie; van Hooff, Marcel H. A.; Friederich, Jaap; Smeenk, Jesper M. J.; Kwee, Janet; Verhoeve, Harold R.; Lambalk, Cornelis B.; Helmerhorst, Frans M.; van der Veen, Fulco; Mol, Ben Willem J.; Torrance, Helen L.; Broekmans, Frank J. M.

    2012-01-01

    Background: Costs of in vitro fertilisation (IVF) are high, which is partly due to the use of follicle stimulating hormone (FSH). FSH is usually administered in a standard dose. However, due to differences in ovarian reserve between women, ovarian response also differs with potential negative conseq

  8. Cloning and gene expression of a cDNA for the chicken follicle-stimulating hormone (FSH)-beta-subunit.

    Science.gov (United States)

    Shen, San-Tai; Yu, John Yuh-Lin

    2002-02-15

    Follicle-stimulating hormone (FSH) is a member of pituitary glycoprotein hormones that are composed of two dissimilar subunits, alpha and beta. Very little information is available regarding the nucleotide and amino acid sequence of FSH-beta in avian species. For better understanding of the phylogenic diversity and evolution of FSH molecule, we have isolated and sequenced the complete complementary DNA (cDNA) encoding chicken FSH-beta precursor molecule by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods. The cloned chicken FSH-beta cDNA consists of 2457-bp nucleotides, including 44-bp nucleotides of the 5'-untranslated region (UTR), 396 bp of the open reading frame, and an extraordinarily long 3'-UTR of 2001-bp nucleotides followed by a poly(A)((16)) tail. It encodes a 131-amino-acid precursor molecule of FSH-beta-subunit with a signal peptide of 20 amino acids followed by a mature protein of 111 amino acids. Twelve cysteine residues, forming six disulfide bonds within beta-subunit and two putative asparagine-linked glycosylation sites, are also conserved in the chicken FSH-beta-subunit. Four proline residues, presumably responsible for changing the backbone direction of protein structure, are conserved in chicken FSH-beta-subunit as well. The nucleotide sequence of chicken FSH-beta cDNA shows high homology with quail FSH-beta cDNA, 97% homology in the open reading frame, and 85% homology in the 3'-UTR. The deduced amino acid sequence of chicken FSH-beta-subunit shows a remarkable similarity to other avian FSH-beta-subunits, 98% homology with quail, and 93% homology with ostrich, whereas a lower similarity (66 to 70%) is noted when compared with mammalian FSH-beta-subunits. By contrast, when comparing with the beta-subunits of chicken luteinizing hormone and thyroid-stimulating hormone, the homologies are as low as 37 and 40%, respectively. FSH-beta mRNA was only expressed in pituitary gland out of various

  9. A FSH-Secreting Pituitary Macroadenoma Causing A Testosterone Deficiency Syndrome

    Directory of Open Access Journals (Sweden)

    Xiong Wang

    2014-03-01

    Full Text Available FSH-secreting pituitary adenomas can affect sexual and reproductive function. In this article, we have reported the case of a 32-year-old male with secondary infertility. The patient had sexual and reproductive disturbances. The test results of the blood samples indicated obviously decreased testosterone (T and estradiol (E2 levels. Based on previous hormonal results, the patient received pituitary stimulation and human chorionic gonadotropin (hCG tests. Both follicle stimulating hormone (FSH and luteinizing hormone (LH showed low response during the pituitary stimulation test. The results of the hCG test indicated that T/E2 could recover to a normal level. In addition, this patient was diagnosed with pituitary macroadenoma, which was supported by the pituitary MRI. The man’s sexual and reproductive functions recovered following surgery. The pathological results confirmed that the tumor tissue was an FSH-secreting pituitary adenoma by immunohistochemical staining. The purpose of this report was to review the relative literature and discuss the influence of FSH-secreting pituitary adenomas on hormones through the hypothalamus-pituitary-testis axis.

  10. Possible applications of RIA of LH and FSH in diagnosis of Stein-Leventhal syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Zenisek, L.; Talas, M.; Stehlikova, J.; Fingerova, H.; Duskova, M.

    1981-01-01

    LH determination in the serum significantly assists in diagnosing polycystic ovaries. Values exceeding 22 mIU/ml serum are indicative of a typical picture of polycystic ovaries similar to those found in the Stein-Leventhal syndrome. Lower levels indicate an atypical picture of polycystic ovaries or low-cyst ovary degeneration. FSH level cannot be used for this diagnosis.

  11. Effects of estradiol and FSH on leptin levels in women with suppressed pituitary

    Directory of Open Access Journals (Sweden)

    Geber Selmo

    2012-06-01

    Full Text Available Abstract Background Female fertility depends on adequate nutrition and energy reserves, suggesting a correlation between the metabolic reserve and reproductive capacity. Leptin regulates body weight and energy homeostasis. The aim of this study was to investigate whether estradiol or FSH alone has a direct effect on the production of leptin. Methods A total of 64 patients submitted to controlled ovarian hyperstimulation with recombinant FSH for assisted reproduction and 20 patients using estradiol valerate for endometrial preparation for oocyte donation treatment were included in the study. All patients used GnRH analogues before starting treatment to achieve pituitary suppression. Blood samples for hormonal measurements were collected before starting and after completing the respective treatments. Data were analyzed statistically by the chi-square test, Student’s t-test and Pearson’s correlation test. Results We observed an elevation of serum leptin levels secondary to the increase in estradiol, in the absence of influence of any other ovarian or pituitary hormone. The rising rate of leptin levels was higher in women treated with recombinant FSH, which also had higher levels of estradiol, than in those treated with estradiol valerate. Conclusions This study demonstrates a correlation between serum levels of estradiol and leptin, suggesting that estradiol is an important regulator of leptin production and that its effects can be amplified by its association with FSH.

  12. Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH

    DEFF Research Database (Denmark)

    Andrade, Evelyn Rabelo; Hyttel, Poul; Landim-Alvarenga, Fernanda Da Cruz;

    2011-01-01

    The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured...

  13. Mutations and polymorphisms in FSH receptor: functional implications in human reproduction.

    Science.gov (United States)

    Desai, Swapna S; Roy, Binita Sur; Mahale, Smita D

    2013-12-01

    FSH brings about its physiological actions by activating a specific receptor located on target cells. Normal functioning of the FSH receptor (FSHR) is crucial for follicular development and estradiol production in females and for the regulation of Sertoli cell function and spermatogenesis in males. In the last two decades, the number of inactivating and activating mutations, single nucleotide polymorphisms, and spliced variants of FSHR gene has been identified in selected infertile cases. Information on genotype-phenotype correlation and in vitro functional characterization of the mutants has helped in understanding the possible genetic cause for female infertility in affected individuals. The information is also being used to dissect various extracellular and intracellular events involved in hormone-receptor interaction by studying the differences in the properties of the mutant receptor when compared with WT receptor. Studies on polymorphisms in the FSHR gene have shown variability in clinical outcome among women treated with FSH. These observations are being explored to develop molecular markers to predict the optimum dose of FSH required for controlled ovarian hyperstimulation. Pharmacogenetics is an emerging field in this area that aims at designing individual treatment protocols for reproductive abnormalities based on FSHR gene polymorphisms. The present review discusses the current knowledge of various genetic alterations in FSHR and their impact on receptor function in the female reproductive system.

  14. Stress inhibits seasonal and FSH-induced ovarian recrudescence in the lizard,Mabuya carinata.

    Science.gov (United States)

    Ganesh, C B; Yajurvedi, H N

    2002-06-01

    Stressors (handling, chasing, and noise) applied randomly five times per day for one month to lizards during the recrudescence phase of the ovarian cycle caused a significant reduction in mean number of oocytes and primordial follicles when compared to those of controls. Further, vitellogenic follicles were absent in the ovary of lizards subjected to stressors. Administration of bovine FSH during post-breeding regression phase of the ovarian cycle induced ovarian recrudescence as shown by significant increases in the mean number of oogonia, oocytes, and primordial follicles compared to controls, as well as vitellogenic growth of follicles. However, lizards treated with FSH and exposed to stressors did not exhibit ovarian recrudescence. Furthermore, FSH administration during the post-breeding regression phase caused a significant increase in serum levels of estradiol compared to controls, which was accompanied by significant increases in the relative weight of the liver and oviduct, as well as vitellogenic growth of follicles. Despite administration of FSH to lizards subjected to stressors, there was neither any increase in serum levels of estradiol and weight of the liver nor vitellogenic growth of follicles. The results indicate that repeated application of stressors inhibits vitellogenic growth of follicles by suppression of steroidogenic activity in M. carinata. This is the first report revealing that the ovary does not respond to gonadotrophin treatment under stressful conditions in reptiles.

  15. Novel FSHβ mutation in a male patient with isolated FSH deficiency and infertility.

    Science.gov (United States)

    Zheng, Junjie; Mao, Jiangfeng; Cui, Mingxuan; Liu, Zhaoxiang; Wang, Xi; Xiong, Shuyu; Nie, Min; Wu, Xueyan

    2017-06-01

    Isolated follicle stimulating hormone (FSH) deficiency due to mutations in FSHβ is an extremely rare autosomal recessive disease that has only been reported in ten patients to date. Symptoms of the disease include amenorrhoea and hypogonadism in women and azoospermia and normal testosterone levels in men. This study describes a Chinese male patient who presented with cryptorchidism and infertility. His serum hormonal profile revealed low FSH, elevated LH and normal testosterone levels. Sequence analysis identified a novel homozygous mutation in the FSHβ gene (c.343C > T) predicted to result in a premature termination codon and a truncated FSH protein (p.R115X). Both parents were heterozygous carriers of the mutation with normal pubertal development and fertility. The patient's testicular volume increased after one year of exogenous FSH replacement therapy at which point spermatocytes were detected in seminal samples, indicating potential future spermatogenesis. The expanded spectrum of FSHβ mutations and associated clinical manifestations described in this study may improve the diagnosis and treatment of this disease. Copyright © 2017. Published by Elsevier Masson SAS.

  16. FSHbeta gene mutation in a female with delayed puberty and hypogonadism: response to recombinant human FSH.

    Directory of Open Access Journals (Sweden)

    S Alain

    2010-01-01

    Full Text Available We report a woman with primary amenorrhoea and infertility associated with an isolated deficiency of pituitary FSH that does not respond to GnRH administration. Serum inhibin B was undetectable and antimullerian hormone (AMH was within the normal range. Ultra sound examination revealed a small uterus and small ovaries with few small follicles. We identified an homozygous 1-bp (G deletion at codon 79 in FSHbeta gene suggesting a complete loss of function. The patient underwent studies of ovarian responsiveness to recombinant human FSH according to the following protocol: 150UI/d for five days following by 75 UI/d for 10 days. Estradiol plasma level started to increase from day 5 associated to a sharp increase of inhibine B and a decrease of LH. During the same time, we observed an excessive development of multiple follicles resulting in an arrest of the treatment to avoid hyperstimulation. The present study confirm that follicles up to 5 mm in diameter had developed in the absence of FSH and that FSH is required for the growth of follicles beyond the two-layer granulose stage.

  17. Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism.

    Science.gov (United States)

    Ruvolo, Giovanni; Roccheri, Maria Carmela; Brucculeri, Anna Maria; Longobardi, Salvatore; Cittadini, Ettore; Bosco, Liana

    2013-04-01

    An observational clinical and molecular study was designed to evaluate the effects of the administration of recombinant human FSH on sperm DNA fragmentation in men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. In the study were included 53 men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. In all patients, sperm DNA fragmentation index (DFI), assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) in situ DNA nick end-labelling (TUNEL) assay, was evaluated before starting the treatment with 150 IU of recombinant human FSH, given three times a week for at least 3 months. Patients' semen analysis and DNA fragmentation index were re-evaluated after the 3-month treatment period. After recombinant human FSH therapy, we did not find any differences in terms of sperm count, motility and morphology. The average DNA fragmentation index was significantly reduced (21.15 vs 15.2, p15 %), while no significant variation occurred in the patients with DFI values ≤ 15 %. Recombinant human FSH administration improves sperm DNA integrity in hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia men with DNA fragmentation index value >15 % .

  18. A STUDY ON SERUM FSH, LH AND PROLACTIN LEVELS AMONG INFERTILE WOMEN

    Directory of Open Access Journals (Sweden)

    Prasad Bheem, Parmar Dinesh, Sharma Nc

    2015-10-01

    Full Text Available Background: Study of hormonal imbalance and its implications in female infertility are an interesting area that requires to be explored in recent time. Hormonal imbalance can associated with irregular menstrual cycle, Amenorrhea, obesity and infertility in women. Other medical conditions such as polycystic ovarian syndrome, Endometriosis, stress, sexually transmitted diseases and chromosomal anomalies may be responsible for infertility in females. Objective: The aim of the present study was to evaluate the serum levels of Follicle Stimulating hormone (FSH, Luteinizing hormone (LH and Prolactin hormone in infertile women that were referred from different infertility clinics and centres. Materials and Methods: This study comprises total 176 female subjects with age ranging from 20 to 40 years and divided in two groups. The total number of 88 infertile women along with 88 fertile women as controls was included for the present study. Serum FSH, LH and Prolactin levels were estimated by enzyme-linked immunosorbent assay (ELISA methods. Results: The results showed maximum infertile women were found between the age group of 30-40 years. The Serum FSH, LH and Prolactin levels among infertile women was 8.77±4.65, 7.64±5.16 and 18.59±7.50 respectively. Whereas, levels of FSH, LH and Prolactin in fertile women showed that 6.71±4.12, 5.66±3.17 and 13.44±5.82 respectively. Conclusion: In this study, we found that the hormone levels have statistically significant with female infertility. The elevated levels of FSH, LH and Prolactin may be one of the important causes for infertility in women.

  19. PENGARUH PEMBERIAN MONOSODIUM GLUTAMAT (MSG PADA TIKUS JANTAN (Rattus Norvegicus TERHADAP FSH DAN LH

    Directory of Open Access Journals (Sweden)

    Zulkarnain Edward

    2010-09-01

    Full Text Available AbstrakKemajuan teknologi informasi membawa dampak terhadap perubahan gaya hidup masyarakat, termasuk perubahan pola konsumsi makanan yang lebih banyak mengkonsumsi jenis makanan cepat saji, makanan kemasan dan makanan awetan yang belakangan ini semakin banyak dijual dipasar tradisional dan swalayan. Penggunaan bahan tambahan makanan sering dijumpai, salah satunya adalah bahan penyedap yang banyak sekali digunakan seperti senyawa L-asam glutamat yang digunakan dalam bentuk garam yaitu monosodium glutamat (MSG. Berbagai merk dagang MSG telah dikenal dimasyarakat secara luas seperti ajinomoto, vetsin, micin, sasa, miwon dan sebagainya.MSG adalah garam monosodium dengan asam glutamat yang sering digunakan sebagai bahan penyedap masakan untuk merangsang selera makan. Pemberian MSG mengakibatkan gangguan hormonal pada hewan coba, ion glutamat dalam sirkulasi portal akan mempengaruhi hipotalamus dalam memproduksi GnRH yang selanjutnya akan mengganggu hipofise anterior dalam memproduksi FSH dan LH. Fungsi FSH adalah untuk bekerja pada tubulus seminiferus terutama pada sel sertoli untuk meningkatkan spermatogenesis, sedangkan LH berfungsi pada sel Leydig untuk mengatur sekresi testosteron.Penelitian ini bersifat eksperimen dengan rancangan post only group design. Penelitian dilakukan di laboratorium Biologi dan laboratorium Biokimia Fakultas Kedokteran Unand Padang dari tanggal 20 Desember 2009 sampai 30 Februari 2010. Populasi adalah tikus putih jantan strain Jepang (Rattus norvegicus yang berasal dari laboratorium Fakultas Matematika dan Ilmu Pengetahuan Alam Unand. Sampel berjumlah 20 ekor dibagi atas 4 kelompok dengan satu kelompok kontrol dan tiga kelompok perlakuan. Dosis MSG yang digunakan yaitu P1= 4800 mg/kgbb/hari, P2=7200 mg/kgbb/hari dan P3= 9600 mg/kgbb/hari diberikan peroral sebanyak dua siklus epitel seminiferus. Analisa dengan uji Anova dengan derajat kepercayaan 95% dan jika bermakna dilanjutkan dengan uji Multiple Comparissons jenis

  20. Social success of in-stream habitat improvement: from fisheries enhancement to the delivery of multiple ecosystem services

    Directory of Open Access Journals (Sweden)

    Maare Marttila

    2016-03-01

    Full Text Available Stream restoration often aims to enhance fisheries by improving stream conditions for target fish species. However, river restoration has a potential impact on a variety of ecosystem services. Among stakeholders, the emerging expectations about restoration attain different priorities. How well these expectations are met influences social perceptions of success or failure. Although public support for restoration is known to have a significant impact on the sustainability and overall success of restoration, social aspects are rarely considered in this context. To address these issues, we conducted a questionnaire study among the residents and fishermen of three recently restored rivers in Finland. Results indicate that both user groups highly supported the restoration goals, but they were not always satisfied with the restoration outcomes. The changes in landscape value and amenity and fish provisioning had the highest influence on the user groups' attitudes. Restoration-induced changes in ecosystem services showed clear variation between the different locations, but the differences in the perceptions of the two user groups were less evident. Comparing perceptions between the user groups and locations and applying the ecosystem services approach are a novel contribution to the debate on restoration success. Our study highlights the importance of perspective, social-ecological context, and adequate communication for success.

  1. Recombinant versus highly-purified, urinary follicle-stimulating hormone (r-FSH vs. HP-uFSH in ovulation induction: a prospective, randomized study with cost-minimization analysis

    Directory of Open Access Journals (Sweden)

    Grassi Giuseppina

    2006-07-01

    Full Text Available Abstract Background Both recombinant FSH (r-FSH and highly-purified, urinary FSH (HP-uFSH are frequently used in ovulation induction associated with timed sexual intercourse. Their effectiveness is reported to be similar, and therefore the costs of treatment represent a major issue to be considered. Although several studies about costs in IVF have been published, data obtained in low-technology infertility treatments are still scarce. Methods Two hundred and sixty infertile women (184 with unexplained infertility, 76 with CC-resistant polycystic ovary syndrome at their first treatment cycle were randomized and included in the study. Ovulation induction was accomplished by daily administration of rFSH or HP-uFSH according to a low-dose, step-up regimen aimed to obtain a monofollicular ovulation. A bi- or tri-follicular ovulation was anyway accepted, whereas hCG was withdrawn and the cycle cancelled when more than three follicles greater than or equal to 18 mm diameter were seen at ultrasound. The primary outcome measure was the cost of therapy per delivered baby, estimated according to a cost-minimization analysis. Secondary outcomes were the following: monofollicular ovulation rate, total FSH dose, cycle cancellation rate, length of the follicular phase, number of developing follicles (>12 mm diameter, endometrial thickness at hCG, incidence of twinning and ovarian hyperstimulation syndrome, delivery rate. Results The overall FSH dose needed to achieve ovulation was significantly lower with r-FSH, whereas all the other studied variables did not significantly differ with either treatments. However, a trend toward a higher delivery rate with r-FSH was observed in the whole group and also when results were considered subgrouping patients according to the indication to treatment. Conclusion Considering the significantly lower number of vials/patient and the slight (although non-significant increase in the delivery rate with r-FSH, the cost

  2. Superovulation with a single administration of FSH in aluminum hydroxide gel: a novel superovulation method for cattle.

    Science.gov (United States)

    Kimura, Koji

    2016-10-18

    Superovulation (SOV) is a necessary technique to produce large numbers of embryos for embryo transfer. In the conventional methods, follicular stimulating hormone (FSH) is administered to donor cattle twice daily for 3 to 4 days. As this method is labor intensive and stresses cattle, improving this method has been desired. We previously developed a novel and simple SOV method, in which the intramuscular injection of a single dose of FSH in aluminum hydroxide gel (AH-gel) induced the growth of multiple follicles, ovulation and the production of multiple embryos. Here we show that AH-gel can efficiently adsorb FSH and release it effectively in the presence of BSA, a major interstitial protein. When a single intramuscular administration of the FSH and AH-gel mixture was performed to cattle, multiple follicular growth, ovulation and embryo production were induced. However, the treatments caused indurations at the administration sites in the muscle. To reduce the muscle damage, we investigated alternative administration routes and different amounts of aluminum in the gel. By administering the FSH in AH-gel subcutaneously rather than intramuscularly, the amount of aluminum in the gel could be reduced, thus reducing the size of the induration. Moreover, repeated administrations of FSH with AH-gel did not affect the superovulatory response. These results indicate that a single administration of FSH with AH-gel is an effective, novel and practical method for SOV treatment.

  3. Beta-endorphin disrupts seasonal and FSH-induced ovarian recrudescence in the lizard Mabuya carinata.

    Science.gov (United States)

    Ganesh, C B; Yajurvedi, H N

    2003-10-01

    Administration (ip) of an opioid peptide, beta-endorphin (beta-EP) (0.1, 0.5, or 1 microg beta-EP/day/lizard for 30 days) during seasonal recrudescence phase of the ovarian cycle inhibited ovarian recrudescence as shown by the absence of vitellogenic follicles in the ovary in contrast to their presence in treatment controls in the lizard Mabuya carinata. In the germinal bed, treatment of 0.1 microg beta-EP did not affect primordial follicles, whereas their mean number was significantly lower in lizards treated with 0.5 or 1 microg beta-EP compared to those of treatment controls. There was also suppression of oviductal development as shown by a significantly lower relative weight of the oviduct and regressed oviductal glands in lizards treated with all the dosages of beta-EP compared to treatment controls. In another experiment, administration of FSH (10 IU FSH/alternate day/lizard for 30 days) during the regression phase of the ovarian cycle induced development of vitellogenic follicles, whereas the treatment controls showed only previtellogenic follicles. In addition, there was a significant increase in the ovarian and oviductal weights compared to initial and treatment controls. However, simultaneous administration of similar dosage of FSH and beta-EP (0.5 microg/day/lizard) did not induce ovarian recrudescence as shown by the absence of vitellogenic follicles in the ovary and significantly lower weight of the ovary and the oviduct and the mean number of oogonia, oocytes, and primordial follicles compared to those of FSH-treated lizards. The results indicate that beta-EP inhibits seasonal as well as FSH-induced ovarian recrudescence. Inhibitory effect of beta-EP on follicular development despite FSH administration implies its effect at the ovarian level in M. carinata. While adversely affecting the ovarian follicular development, beta-EP did not affect the adrenal gland as there was no significant variation in the mean nuclear diameter of the adrenocortical cells

  4. Combined beta FSH and beta LH response to TRH in patients with clinically non-functioning pituitary adenomas.

    Science.gov (United States)

    Somjen, D; Tordjman, K; Kohen, F; Baz, M; Razon, N; Ouaknine, G; Stern, N

    1997-05-01

    'Paradoxical' responses of LH, FSH, alpha-subunits and beta LH to TRH have previously been reported in individuals with clinically non-functioning pituitary tumours (NFT). The present study was designed to assess the in vivo and in vitro responses of beta FSH to TRH in NFT. We further examined the possibility that a TRH challenge with combined measurement of beta FSH and beta LH will identify a common anomalous secretory pattern in patients with NFT. Forty patients with NFT underwent a standard TRH test (400 micrograms intravenously). Blood samples for the determination of beta FSH, beta LH, FSH and LH were collected prior to TRH as well as 15, 30, 45, 60 and 90 minutes following injection. Additionally, cultured adenomatous cells from eight to these patients were exposed to TRH in the absence and presence of octreotide and gonadotropin subunits were determined. TRH elicited a marked rise in circulating beta FSH in 29 of 40 individuals and in beta LH in 28 of 36 patients with NFT. In a subgroup of eight individuals whose tumours were harvested during surgery and cultured for 7-21 days, TRH increased beta FSH or beta LH and alpha-subunit release in cultured adenomatous cells in all cases, including tumours from subjects not responding to TRH in vivo. In this subgroup of patients octreotide inhibited basal beta FSH secretion but not basal beta LH secretion both in vivo and in primary cultures of NFT cells. Both the in vivo and in vitro beta FSH, beta LH and alpha-subunit responses to TRH were entirely inhibited by octreotide. In all, 38 of the 40 subjects could be identified by either elevated basal beta FSH or beta LH levels and/or an abnormal rise in either beta FSH or beta LH in response to TRH. The measurement of basal and TRH-stimulated beta-FSH and beta-LH levels identifies an abnormal hormonal secretory pattern in the vast majority (> 90%) of patients with clinically nonfunctioning pituitary tumours.

  5. A ranking of coastal EU habitat types using Ecosystem Services

    NARCIS (Netherlands)

    Smith, S.R.; Deerenberg, C.M.

    2012-01-01

    During hydraulic works it is not uncommon that interchanging of habitat types occurs through partial area loss of the habitat type at the location of the activity and development of new habitat or enhancing the quality of the same of another habitat type elsewhere. According to the current interpret

  6. Do FSH/LH ratio and gonadal hormone levels predict clinical improvement in postmenopausal schizophrenia women?

    Science.gov (United States)

    González-Rodríguez, Alexandre; Bernardo, Miquel; Penadés, Rafael; Arias, Bárbara; Ruiz Cortés, Victoria; Seeman, Mary V; Catalán, Rosa

    2017-07-12

    Menopause is a process characterized by a decline in estrogen levels and is therefore a period of biological vulnerability for psychotic relapse in women with schizophrenia. Our goal was to correlate not only gonadal hormone levels but also follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels with improvement in specific clinical symptoms. Thirty-seven acutely ill postmenopausal schizophrenia women with a newly initiated, clinically determined change in antipsychotic medication participated in a 12-week prospective observational outcome study. Scales used were the PANSS scale for psychotic symptoms, the PSP for functioning, and CGI for global clinical impression. Circulating FSH, LH, estradiol, progesterone, and testosterone serum levels were determined by chemiluminescent immunoassay. Partial correlational analyses were performed along with a Bonferroni significance correction (p schizophrenia symptom domains, we recommend further investigation of pituitary, adrenal, and gonadal hormone ratios as potential markers of clinical improvement in this population.

  7. Human Menopausal Gonadotropin versus Recombinant FSH in Polycystic Ovary Syndrome Patients Undergoing In Vitro Fertilization

    Directory of Open Access Journals (Sweden)

    Sertac Batioglu

    2013-01-01

    Full Text Available Background: We aimed to compare human menopausal gonadotropin (hMG and recombinantfollicle-stimulating hormone (r FSH with respect to clinical outcomes and the development ofovarian hyperstimulation syndrome (OHSS for patients with polycystic ovary syndrome (PCOStreated with in vitro fertilization (IVF.Materials and Methods: This prospective randomized controlled trial included a total of 80 womenwith PCOS. Of these, 38 were randomized to receive treatment with hMG and 42 with rFSH usinga long gonadotropin releasing hormone (GnRH analogue protocol. Outcome measures were cyclecharacteristics, pregnancy rates, the need for coasting, and OHSS rates.Results: In the hMG group we observed a significantly lower peak estradiol (E2 level (p=0.02,fewer intermediate-sized follicles (p=0.001, lower number of oocytes retrieved (p=0.002 andmetaphase II (MII oocytes (p=0.003. However, there were no significant differences between thegroups in the number of fertilized oocytes, fertilization rates, top quality embryo counts, and thenumber of transferred embryos. There was no difference in pregnancy rates between the groups.OHSS occurred in 11.9% of the rFSH group patients, whereas no OHSS developed in the hMGgroup. Coasting requirements were lower in the hMG group (19.2% vs. 48.9%, p=0.013.Conclusion: Ovarian stimulation with hMG and rFSH provides similar clinical pregnancy rates inPCOS patients treated with a long GnRH agonist protocol in IVF cycles. hMG stimulation appearsto be associated with a lower rate of OHSS and decreased coasting requirements (RegistrationNumber: NCT01365936.

  8. An FSH and TSH pituitary adenoma, presenting with precocious puberty and central hyperthyroidism

    OpenAIRE

    Guadalupe Vargas; Lourdes-Josefina Balcazar-Hernandez; Virgilio Melgar; Roser-Montserrat Magriña-Mercado; Baldomero Gonzalez; Javier Baquera; Moisés Mercado

    2017-01-01

    A 19-year-old woman with a history of isosexual precocious puberty and bilateral oophorectomy at age 10 years because of giant ovarian cysts, presents with headaches and mild symptoms and signs of hyperthyroidism. Hormonal evaluation revealed elevated FSH and LH levels in the postmenopausal range and free hyperthyroxinemia with an inappropriately normal TSH. Pituitary MRI showed a 2-cm macroadenoma with suprasellar extension. She underwent successful surgical resection of the pituitary tumor,...

  9. Effects of recombinant LH treatment on folliculogenesis and responsiveness to FSH stimulation

    DEFF Research Database (Denmark)

    Durnerin, Cedrin I; Erb, K; Fleming, R

    2008-01-01

    BACKGROUND: The role of LH in sensitizing antral follicles to FSH is unclear. LH is required for normal hormone production and normal oocyte and embryo development, but follicular responses to LH may depend upon the stage of development. Potential roles at the early follicular phase were explored...... in a clinical setting by employing a sequential approach to stimulation by recombinant human (r-h) LH followed by r-hFSH in women who were profoundly down-regulated by depo GnRH agonist. METHODS: We employed a multi-centre, prospective, randomized approach. Women (n = 146) were treated in a long course high......-dose GnRH agonist (Decapeptyl, 4.2 mg s.c.) protocol and were randomized to receive r-hLH (Luveris, 300 IU/day) for a fixed 7 days, or no r-hLH treatment. This was followed by a standard r-hFSH stimulation regime (Gonal-F, 150 IU/day). Ultrasound and hormone assessments of responses were measured...

  10. Primary ovarian insufficiency in classic galactosemia: role of FSH dysfunction and timing of the lesion.

    Science.gov (United States)

    Gubbels, Cynthia S; Land, Jolande A; Evers, Johannes L H; Bierau, Jörgen; Menheere, Paul P C A; Robben, Simon G F; Rubio-Gozalbo, M Estela

    2013-01-01

    FSH inactivity due to secondary hypoglycosylation has been suggested as a potential mechanism for primary ovarian insufficiency in classic galactosemia. To investigate the role of FSH and to gain insight in the timing of the damage, ovarian stimulation tests were performed and data on ovarian imaging collected. Fifteen patients with primary ovarian insufficiency underwent ovarian stimulation with gonadotropins. Only one patient showed a normal increase in estradiol level, all the others had a low or no estradiol response. Anti-Müllerian hormone measurement in all girls and women showed levels below the detection limit of 0.10 μg/l. Ovarian volumes were evaluated by MRI in 14 patients and compared to age matched controls, prepubertal controls and postmenopausal controls. The ovarian volumes of the galactosemic girls were smaller than those of the age matched controls (p = 0.001) and the prepubertal ovaries (p = 0.008), and did not differ significantly from postmenopausal ovarian volumes (p = 0.161). In conclusion we found no evidence that FSH inactivity plays a role in primary ovarian insufficiency in classic galactosemia. Moreover, ovarian imaging results point to an early onset of ovarian failure in this disease.

  11. Follicle development and FSH secretion pattern of Ongole crossbred cow with natural twin birth history

    Directory of Open Access Journals (Sweden)

    Aryogi

    2013-10-01

    Full Text Available A research was done to obtain basic data about influence of natural twin birth history on reproduction of Ongole crossbred (PO cow, as a basic consideration in utilizing twin genetic potency to increase beef production. The research was done for three estrus cycles respectively, and consists of two activities. The first activity was done in Beef Cattle Research Station (BCReS at Grati sub-district, Pasuruan district – East Java, to observe number and development of follicle of ten cows with natural twin birth history (TP and ten cows with single birth history (SP. In this activit ultra sonography (USG equipment was used. The second activity was done in BCReS and in the Faculty of Veterinary Airlangga University at Surabaya, to observe concentration and profile of FSH using Bovine Blood Serum kit and IRMA method of five cows with TP and five PO cows with SP. Data obtained were analyzed using Chi-square test, t-test and descriptive presentation. Result shows that 23.33% of cows with TP produced two dominant (de Graf follicles in an estrus cycle and significantly (P < 0.01 higher FSH secretion concentration (1.26 – 3.13 times than that of cows with SP. It is concluded that PO cows with TP can produces more than one de Graf follicles in an estrus cycle and it has very high secretion concentration level of FSH.

  12. Inter-laboratory validation of the measurement of follicle stimulating hormone (FSH after various lengths of frozen storage

    Directory of Open Access Journals (Sweden)

    Behr Barry

    2010-11-01

    Full Text Available Abstract Background Serum follicle stimulating hormone (FSH levels are used clinically to evaluate infertility, pituitary and gonadal disorders. With increased frequency of research collaborations across institutions, it is essential that inter-laboratory validation is addressed. Methods An inter-laboratory validation of three commercial FSH immunoassays was performed with human serum samples of varying frozen storage length (2 batches of 15 samples each at -25 degree C. Percentage differences and Bland-Altman limits of agreement were calculated. Results The inter- and intra-laboratory consistency of FSH values with the same assay manufacturer was much higher after shorter-term storage (frozen for less than 11 months, mean percentage degradation less than 4% than after long-term storage (2-3 years, mean percentage degradation = 23%. Comparing assay results from different manufacturers, there was similar overall long term degradation as seen with the same manufacturer (-25%, however the degradation was greater when the original FSH was greater than 20 mIU/mL relative to less than 10 mIU/mL (p Conclusion The findings suggest that degradation of serum samples stored between 11 months and 2-3 years at -25 degrees C can lead to unstable FSH measurements. Inter-laboratory variability due to frozen storage time and manufacturer differences in assay results should be accounted for when designing and implementing research or clinical quality control activities involving serum FSH at multiple study sites.

  13. Selective effects of charge on G protein activation by FSH-receptor residues 551-555 and 650-653.

    Science.gov (United States)

    Grasso, P; Deziel, M R; Reichert, L E

    1995-01-01

    Two cytosolic regions of the rat testicular FSH receptor (FSHR), residues 533-555 and 645-653, have been identified as G protein-coupling domains. We localized the activity in these domains to their C-terminal sequences, residues 551-555 (KIAKR, net charge +3) and 650-653 (RKSH, net charge +3), and examined the effects of charge on G protein activation by the C-terminal peptides, using synthetic analogs containing additions, through alanine (A) linkages, of arginine (R, +), histidine (H, +) or both. RA-KIAKR (net charge +4) mimicked the effect of FSHR-(551-555) on guanine nucleotide exchange in rat testis membranes, but reduced its ability to inhibit FSH-stimulated estradiol biosynthesis in cultured rat Sertoli cells. Further increasing net charge by the addition of H (HARA-KIAKR, net charge +5) increased guanosine 5'-triphosphate (GTP) binding, but eliminated FSHR-(551-555) effects on FSH-stimulated steroidogenesis. HA-RKSH (net charge +4) significantly inhibited guanine nucleotide exchange in rat testis membranes, but stimulated basal and potentiated FSH-induced estradiol biosynthesis in cultured rat Sertoli cells. Addition of two H residues (HAHA-RKSH, net charge +5) restored GTP binding and further potentiated basal and FSH-stimulated steroidogenesis. These results suggest that positive charges in G protein-coupling domains of the FSHR play a role in modulating G protein activation and postbinding effects of FSH, such as steroidogenesis.

  14. Comparison of marmoset and human FSH using synthetic peptides of the β-subunit L2 loop region and anti-peptide antibodies.

    Science.gov (United States)

    Kutteyil, Susha S; Kulkarni, Bhalchandra J; Mojidra, Rahul; Joseph, Shaini; Pathak, Bhakti R; Mahale, Smita D

    2016-06-01

    Follicle stimulating hormone (FSH) is a glycoprotein hormone required for female and male gametogenesis in vertebrates. Common marmoset (Callithrix jacchus) is a New World primate monkey, used as animal model in biomedical research. Observations like, requirement of extremely high dose of human FSH in marmosets for superovulation compared to other primates and generation of antibodies in marmoset against human FSH after repeated superovulation cycles, point towards the possibility that FSH-FSH receptor (FSHR) interaction in marmosets might be different than in the humans. In this study we attempted to understand some of these structural differences using FSH peptides and anti-peptide antibody approach. Based on sequence alignment, in silico modeling and docking studies, L2 loop of FSH β-subunit (L2β) was found to be different between marmoset and human. Hence, peptides corresponding to region 32-50 of marmoset and human L2β loop were synthesized, purified and characterized. The peptides displayed dissimilarity in terms of molecular mass, predicted isoelectric point, predicted charge and in the ability to inhibit hormone-receptor interaction. Polyclonal antibodies generated against both the peptides were found to exhibit specific binding for the corresponding peptide and parent FSH in ELISA and Western blotting respectively and exhibited negligible reactivity to cross-species peptide and FSH in ELISA. The anti-peptide antibody against marmoset FSH was also able to detect native FSH in marmoset plasma samples and pituitary sections. In summary, the L2β loop of marmoset and human FSH has distinct receptor interaction ability and immunoreactivity indicating possibility of subtle conformational and biochemical differences between the two regions which may affect the FSH-FSHR interaction in these two primates. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.

  15. Effects of ecosystem development on benthic secondary production in restored and created mangrove habitats

    Science.gov (United States)

    Wetland creation, enhancement, and restoration activities are commonly implemented to compensate for wetland loss or degradation. However, functional equivalence in restored and created wetland habitats is often poorly understood. In estuarine habitats, changes in habitat qualit...

  16. Two-dimensional physical habitat modeling of effects of habitat structures on urban stream restoration

    Directory of Open Access Journals (Sweden)

    Dongkyun IM

    2011-12-01

    Full Text Available River corridors, even if highly modified or degraded, still provide important habitats for numerous biological species, and carry high aesthetic and economic values. One of the keys to urban stream restoration is recovery and maintenance of ecological flows sufficient to sustain aquatic ecosystems. In this study, the Hongje Stream in the Seoul metropolitan area of Korea was selected for evaluating a physically-based habitat with and without habitat structures. The potential value of the aquatic habitat was evaluated by a weighted usable area (WUA using River2D, a two-dimensional hydraulic model. The habitat suitability for Zacco platypus in the Hongje Stream was simulated with and without habitat structures. The computed WUA values for the boulder, spur dike, and riffle increased by about 2%, 7%, and 131%, respectively, after their construction. Also, the three habitat structures, especially the riffle, can contribute to increasing hydraulic heterogeneity and enhancing habitat diversity.

  17. Effects of prepartum lipid supplementation on FSH superstimulation and transferable embryo recovery in multiparous beef cows.

    Science.gov (United States)

    Bader, J F; Kojima, F N; Wehrman, M E; Lindsey, B R; Kerley, M S; Patterson, D J

    2005-01-01

    The objective of this experiment was to determine the effect of prepartum lipid supplementation on the number and quality of embryos recovered following ovarian super-ovulation in postpartum suckled beef cows. Mature cows (n = 40) were assigned to one of two treatments (lipid versus. no lipid) and supplemented for approximately 40 days prior to calving. Supplements provided to cows were isocaloric and isonitrogenous. The treatment group was fed 1.6 kg hd(-1) per day of whole soybeans (WSB; 19.8% ether extract, and 41.8% crude protein) and the control group received a supplement consisting of 1.8 kg hd(-1) day of a soybean meal and soy-hull combination (SBS; 2.15% EE and 36.81% CP). Cows were synchronized using a GnRH [Cystorelin((R)) 100 microg im]-GnRH-PGF(2alpha) [Lutalyse 25 mg im] protocol. Cows were administered two injections of GnRH seven days apart and PG seven days after the second GnRH injection. Twenty-eight cows (WSB, n = 15; SBS, n = 13) responded to estrus synchronization and were superstimulated. Super-ovulation was initiated on day 8-10 of the synchronized cycle by twice-daily injections of pFSH (Pluset) over four days in decreasing doses using a total of 608.4 IU per cow. Prostaglandin F(2alpha) was administered 96 and 108 h after super-stimulation was initiated with FSH. Days postpartum (WSB = 59 days; SBS = 57 days) at initiation of FSH treatments were similar (P > 0.10) for both treatments. Cows were monitored for estrus activity by the HeatWatch Estrus Detection System. Twenty-seven cows (WSB, n = 15; SBS, n = 12) exhibited estrus after FSH and inseminated at 0, 12, and 24 h after the onset of estrus with 1, 2, and 1 units of semen, respectively. Embryos were recovered and evaluated 7-8 days later. Only cows that responded to FSH and that were inseminated were used for statistical analysis. Data were analyzed using the General Linear Models Procedure of SAS. Body condition scores did not differ (P > 0.10) between treatments when cows were

  18. FSH-FSHR3-stem cells in ovary surface epithelium: basis for adult ovarian biology, failure, aging, and cancer.

    Science.gov (United States)

    Bhartiya, Deepa; Singh, Jarnail

    2015-01-01

    Despite extensive research, genetic basis of premature ovarian failure (POF) and ovarian cancer still remains elusive. It is indeed paradoxical that scientists searched for mutations in FSH receptor (FSHR) expressed on granulosa cells, whereas more than 90% of cancers arise in ovary surface epithelium (OSE). Two distinct populations of stem cells including very small embryonic-like stem cells (VSELs) and ovarian stem cells (OSCs) exist in OSE, are responsible for neo-oogenesis and primordial follicle assembly in adult life, and are modulated by FSH via its alternatively spliced receptor variant FSHR3 (growth factor type 1 receptor acting via calcium signaling and the ERK/MAPK pathway). Any defect in FSH-FSHR3-stem cell interaction in OSE may affect folliculogenesis and thus result in POF. Ovarian aging is associated with a compromised microenvironment that does not support stem cell differentiation into oocytes and further folliculogenesis. FSH exerts a mitogenic effect on OSE and elevated FSH levels associated with advanced age may provide a continuous trigger for stem cells to proliferate resulting in cancer, thus supporting gonadotropin theory for ovarian cancer. Present review is an attempt to put adult ovarian biology, POF, aging, and cancer in the perspective of FSH-FSHR3-stem cell network that functions in OSE. This hypothesis is further supported by the recent understanding that: i) cancer is a stem cell disease and OSE is the niche for ovarian cancer stem cells; ii) ovarian OCT4-positive stem cells are regulated by FSH; and iii) OCT4 along with LIN28 and BMP4 are highly expressed in ovarian cancers.

  19. 促性腺激素的同分异构体%FSH isomer and ovarian response

    Institute of Scientific and Technical Information of China (English)

    孙瑜; 朱依敏

    2012-01-01

    Follicle stimulating hormone(FSH) is an important reproductive hormone in human, the FSH isomers have variable biological properties, which can affect the ovarian function through interaction with FSH receptors. Ovarian response to FSH stimulation also depends on FSH receptor genotype. At present,a large number of single nucleotide polymorphisms(SNPs) were investigated in FSH receptors genes, which may cause the difference of ovarian function through the influencing on receptor activities and may be related with ovarian stimulus response in assisted reproductive technology. The determination of gene polymorphism in patients with ovarian stimulation show significances in making the individual programs of ovarian stimulation, which can optimize the treatment of infertility. However more clinical trials are needed to confirm its effectiveness.%卵巢的反应性受多种因素的影响,高龄、卵巢手术、盆腔结核、盆腔炎症、免疫性因素等均可导致卵巢低反应.近年来发现卵泡刺激素(FSH)的同分异构体、FSH受体基因突变及多态性导致FSH受体结构和功能异常,也是卵巢反应异常的原因之一[1].在促排卵过程中,应综合考量可能影响卵巢反应性的各种因素,尤其是FSH异构体的不同生物学特性导致的卵巢反应差异,选择合适的促排卵方案,达到相对较为满意的结局.

  20. Early postnatal treatment of hypogonadotropic hypogonadism with recombinant human FSH and LH

    DEFF Research Database (Denmark)

    Main, Katharina M; Schmidt, Ida M; Toppari, Jorma

    2002-01-01

    postnatally, to mimic the physiological development, would improve testicular growth and fertility potential later in life. DESIGN: Our patient presented with micropenis. Serum hormone concentrations were measured monthly after delivery: LH and testosterone were undetectable, and FSH and inhibin B were below...... to values within normal limits (0.7-1.88 IU/l, 0.17-3.24 IU/l, 121-268 pg/ml and 40-55 pmol/l respectively), whereas serum testosterone remained undetectable. Penile length increased from 1.6 to 2.4 cm and testicular volume, assessed by ultrasound, increased by 170%. No significant adverse events were...

  1. Louisiana ESI: HABITATS (Habitat and Plant Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for coastal habitats in Louisiana. Vector polygons represent various habitats, including marsh types, other...

  2. EcologicHabitat_WLH

    Data.gov (United States)

    Vermont Center for Geographic Information — The Wildlife Linkage Habitat Analysis uses landscape scale data to identify or predict the location of potentially significant wildlife linkage habitats (WLH)...

  3. Mink aging is associated with a reduction in ovarian hormone release and the response to FSH and ghrelin.

    Science.gov (United States)

    Sirotkin, Alexander V; Mertin, Dušan; Süvegová, Karina; Lauričik, Jozef; Morovič, Martin; Harrath, Abdel Halim; Kotwica, Jan

    2016-09-15

    The endocrine mechanisms of mink ovarian hormones release and reproductive aging are poorly investigated. The aims of our study were to: (1) identify hormones produced by mink ovaries (the steroids progesterone [P] and estradiol [E], the peptide hormone oxytocin [OT], and the prostaglandin F [PGF] and prostaglandin E [PGE]); (2) examine the effect of FSH and ghrelin on the release of the hormones listed previously; and (3) understand whether these hormones can be involved in the control of mink reproductive aging, i.e., whether aging can be associated with changes (a) in the basal release of P, E, OT, PGF, or PGE and (b) their response to FSH and ghrelin. Fragments of ovaries of young (yearlings) and old (3-5 years of age) minks were cultured with and without FSH and ghrelin (0, 1, 10, or 100 ng/mL), and the release of hormones was analyzed by EIA/RIA. We found that isolated ovaries were able to release P, E, OT, PGF, and PGE, and the levels of P produced in the ovaries of old animals were lower than those produced in the ovaries of young animals, whereas the levels of other hormones did not differ. FSH was able to stimulate P and E and suppress OT and PGF and did not affect PGE release. Aging was associated with the inhibition of the effect of FSH on ovarian P and E, the appearance of the inhibitory action of FSH on OT, and the disappearance of this action on ovarian PGF. PGE was not affected by FSH, irrespective of animal age. Ghrelin was able to promote E (but not P) and suppress OT, PGF, and PGE output. Aging was associated with the appearance of an inhibitory influence of ghrelin on ovarian OT and PGE and with the disappearance of this influence on PGF output. Aging did not affect the action of ghrelin on ovarian P and E. Our observations (1) confirm the production of P and E and show that OT, PGF, and PGE are released from mink ovaries, (2) confirm the involvement of FSH and demonstrate the involvement of ghrelin in the control of mink ovarian hormone

  4. Evidência da resposta folicular ao FSH pelo hormônio antimülleriano em mulheres ovulatórias Evidence of follicle responsiveness to FSH by antimüllerian hormone in ovulating women

    Directory of Open Access Journals (Sweden)

    Juliano Brum Scheffer

    2009-03-01

    Full Text Available OBJETIVO: examinar a hipótese de que o nível sérico do hormônio antimülleriano (AMH reflete a resposta dos folículos antrais à administração do FSH. MÉTODOS: estudo prospectivo, no qual foram incluídas 116 pacientes normo-ovulatórias inférteis submetidas à hiperestimulação ovariana controlada com agonista de GnRH e FSH. Depois de atingir a supressão pituitária e antes da administração de FSH (dia basal, o nível sérico de AMH foi mensurado. O número de folículos antrais foi determinado pela ultrassonografia no dia basal (folículos antrais precoces; 2 a 8 mm e no dia da administração do hCG (dhCG; folículos pré-ovulatórios; >16 mm. A resposta folicular ao FSH foi determinada pela percentagem de folículos antrais precoces que atingiram os estágios pré-ovulatórios em resposta ao FSH (taxa de maturação. Foram estudadas as correlações do AMH com a idade das pacientes, número total de folículos antrais precoces e pré-ovulatórios, oócitos coletados, dose total de FSH na estimulação ovariana controlada e a taxa de maturação folicular. Para análise estatística, foram usados a regressão simples e o teste de Spearman's, com nível de significância de 5%. RESULTADOS: o nível sérico de AMH foi positivamente correlacionado com o número de folículos antrais precoces no dia basal (r=0,64; pPURPOSE: to test the hypothesis that the anti-müllerian hormone (AMH serum level reflects the antral follicles' response to the administration of FSH. METHODS: prospective study, including 116 normo-ovulatory infertile patients submitted to controlled ovarian hyperstimulation with GnRH and FSH agonists. The AMH serum level was measured after reaching the pituitary suppression and before the FSH administration (basal day. The number of antral follicles was determined by ultrasonography at the basal day (precocious antral follicles; 2 to 8 mm and at the day of hCG administration (dhCG; pre-ovulatory follicles; >16 mm. The

  5. G protein-coupled receptor kinase 2 and beta-arrestins are recruited to FSH receptor in stimulated rat primary Sertoli cells.

    Science.gov (United States)

    Marion, Sébastien; Kara, Elodie; Crepieux, Pascale; Piketty, Vincent; Martinat, Nadine; Guillou, Florian; Reiter, Eric

    2006-08-01

    FSH-receptor (FSH-R) signaling is regulated by agonist-induced desensitization and internalization. It has been shown, in a variety of overexpression systems, that G protein-coupled receptor kinases (GRKs) phosphorylate the activated FSH-R, promote beta-arrestin recruitment and ultimately lead to internalization. The accuracy of this mechanism has not yet been demonstrated in cells expressing these different molecules at physiological levels. Using sucrose gradient fractionation, we show that FSH induces the recruitment of the endogenous GRK 2 and beta-arrestin 1/2 from the cytoplasm to the plasma membrane of rat primary Sertoli cells. As assessed by ligand binding, the FSH-R was found expressed in the fractions where GRK 2 and beta-arrestins were recruited upon FSH treatment. In addition, the endogenous beta-arrestin 1 was found dephosphorylated in an agonist-dependent manner. Finally, a significant FSH-binding activity was co-immunoprecipitated with the endogenous beta-arrestins from agonist-stimulated but not from untreated Sertoli cell extracts. This FSH-R interaction with beta-arrestins was sustained for up to 30 min. In conclusion, our data strongly suggest that the GRK/beta-arrestin machinery plays a physiologically relevant role in the regulation of the FSH signaling.

  6. Study on the Effect of ESR and FSH β Genes on Litter Size in Local Northeast Three Pig Breeds

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The method of PCR-RFLP was used to analyze the effects of ESR and FSH β genotypes on the litter size,especially the corresponding changes with the difference of genotype distributions in the Min,Sanjiang and Junmu I pigs.The results show that the ESR and FSH β genes are the major genes of litter size;the heterozygous genotype for ESR as well as for FSH β locus has a more litter size than others.However,the combined genotype ABDD means a more litter size than other genotypes,the frequencies of the heterozygous genotypes in Sanjiang and Junmu I are lower than in Min which maybe one of the most important reasons for the little litter sizes of those two breeds.

  7. Differential gene expression in human granulosa cells from recombinant FSH versus human menopausal gonadotropin ovarian stimulation protocols

    Directory of Open Access Journals (Sweden)

    Bietz Mandi G

    2010-03-01

    Full Text Available Abstract Background The study was designed to test the hypothesis that granulosa cell (GC gene expression response differs between recombinant FSH and human menopausal gonadotropin (hMG stimulation regimens. Methods Females Results After exclusions, 1736 genes exhibited differential expression between groups. Over 400 were categorized as signal transduction genes, ~180 as transcriptional regulators, and ~175 as enzymes/metabolic genes. Expression of selected genes was confirmed by RT-PCR. Differentially expressed genes included A kinase anchor protein 11 (AKAP11, bone morphogenetic protein receptor II (BMPR2, epidermal growth factor (EGF, insulin-like growth factor binding protein (IGFBP-4, IGFBP-5, and hypoxia-inducible factor (HIF-1 alpha. Conclusions Results suggest that major differences exist in the mechanism by which pure FSH alone versus FSH/LH regulate gene expression in preovulatory GC that could impact oocyte maturity and developmental competence.

  8. Kulm Wetland Management District Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of this habitat management plan (HMP) is to provide a strategic plan for consistently and effectively protecting, acquiring, enhancing, restoring, and...

  9. Recovery of ovarian function after postradiation menopause. Do FSH levels have a definitive prognostic value?; Recuperation de fonctionnement ovarien apres menopause radio-induite. L`hormone folliculostimulante (FSH) a-t-elle une valeur pronostique definitive?

    Energy Technology Data Exchange (ETDEWEB)

    Letur-Konirsch, H. [Hopital Necker-Enfants-Malades, 75 - Paris (France); Guis, F. [Hopital Antoine-Beclere, Clamart (France)

    1996-08-01

    Menopause, conventionally defined as the permanent cessation as a result of loss of ovarian follicular activity, is biologically expressed by the collapse of plasma levels and increased plasma levels of the gonadotrophins FSH (follicle stimulating hormone) and LH (luteinizing hormone). At present, estimation of the ovarian follicle reserve is based on endocrine capacity tests of the ovaries, with increased FSH representing the first sign of exocrine ovarian failure. We report the case of one of our amenorrhoeic patients after chemotherapy, total body radiation and allogeneic bone marrow transplantation for acute immuno-blastic leukaemia. This patient was included in an in vitro fertilization with oocyte donation (IVF-OD) programme for iatrogenic premature ovarian failure with increased FSH levels. Instead of high levels of gonadotrophins, this young woman recovered spontaneous follicular developments, benefited from standard IVF with her own oocytes and brought a twin pregnancy to term. This observation shows that a high FSH level is not a definitive prediction of ovarian exocrine capacity. In young women of child-bearing age such as these wanting a child and showing signs of endogenous estrogen impregnation, evaluation of the existence and quality of follicular development is an important factor. (author). 13 refs.

  10. FSH receptor gene variants are rarely associated with premature ovarian failure.

    Science.gov (United States)

    Woad, Kathryn J; Prendergast, Deborah; Winship, Ingrid M; Shelling, Andrew N

    2013-04-01

    FSH receptor (FSHR) gene variants have been associated with premature ovarian failure (POF). Genomic DNA from New Zealand women with POF (n=80) and control women (n=80) was screened for variants in FSHR exons 7 and 10. FSHR exon 7 variants, including the c.566C>T Finnish founder mutation (p.Ala189Val), were not detected. Previously reported FSHR exon 10 polymorphisms were identified in both groups with similar allelic distributions. A novel heterozygous FSHR exon 10 variant c.1411A>T, p.Ile471Phe was observed in one woman with a family history of POF, but not her affected siblings. It is concluded that variants in exons 7 and 10 of FSHR are not frequently associated with the development of POF in the New Zealand population.

  11. Recombinant LH supplementation to recombinant FSH during the final days of controlled ovarian stimulation for in vitro fertilization. A multicentre, prospective, randomized, controlled trial

    DEFF Research Database (Denmark)

    Nyboeandersen, A.; Humaidan, P.; Fried, G.

    2008-01-01

    BACKGROUND: The purpose of this multicentre, multinational trial was to study whether rLH supplementation to recombinant FSH (rFSH) during the late follicular phase increased pregnancy rates. METHODS: After down-regulation with nafarelin, 526 women were randomized on Day 1 of stimulation to use...

  12. A common polymorphic allele of the LH beta-subunit gene is associated with higher exogenous FSH consumption during controlled ovarian stimulation for assisted reproductive technology

    DEFF Research Database (Denmark)

    Alviggi, Carlo; Pettersson, Kim; Longobardi, Salvatore

    2013-01-01

    a long GnRH-agonist down-regulation protocol received an individualized dose of r-hFSH (100 IU and 375 IU s.c. daily) according to antral follicle count, baseline FSH, body mass index and age. The LH genotype was assessed in all patients by immunofluorometric assay. RESULTS: V-betaLH was present in 11...

  13. Increased basal and pulsatile secretion of FSH and LH in young men with 47,XXY or 46,XX karyotypes

    DEFF Research Database (Denmark)

    Aksglaede, L.; Jensen, Rikke Bodin Beck; Carlsen, E.;

    2008-01-01

    basal, pulsatile, and total LH and FSH secretion were associated with significantly more LH peaks per 24 h in comparison with healthy controls. Thus, our data indicate that in patients with Klinefelter syndrome and XX male karyotypes the entire hypothalamic-pituitary-gonadal axis has undergone...

  14. The OPTIMIST study: optimisation of cost effectiveness through individualised FSH stimulation dosages for IVF treatment. A randomised controlled trial

    Directory of Open Access Journals (Sweden)

    van Tilborg Theodora C

    2012-09-01

    Full Text Available Abstract Background Costs of in vitro fertilisation (IVF are high, which is partly due to the use of follicle stimulating hormone (FSH. FSH is usually administered in a standard dose. However, due to differences in ovarian reserve between women, ovarian response also differs with potential negative consequences on pregnancy rates. A Markov decision-analytic model showed that FSH dose individualisation according to ovarian reserve is likely to be cost-effective in women who are eligible for IVF. However, this has never been confirmed in a large randomised controlled trial (RCT. The aim of the present study is to assess whether an individualised FSH dose regime based on an ovarian reserve test (ORT is more cost-effective than a standard dose regime. Methods/Design Multicentre RCT in subfertile women indicated for a first IVF or intracytoplasmic sperm injection cycle, who are aged  Discussion The results of this study will be integrated into a decision model that compares cost-effectiveness of the three dose-adjustment strategies to a standard dose strategy. The study outcomes will provide scientific foundation for national and international guidelines. Trial registration NTR2657

  15. Concanavalin-A induces granulosa cell death and inhibits FSH-mediated follicular growth and ovarian maturation in female rats.

    Science.gov (United States)

    Velasquez, Ethel V; Ríos, Mariana; Ortiz, María Elena; Lizama, Carlos; Nuñez, Elizabeth; Abramovich, Dalhia; Orge, Felipe; Oliva, Barbara; Orellana, Renán; Villalon, Manuel; Moreno, Ricardo D; Tesone, Marta; Rokka, Anne; Corthals, Garry; Croxatto, Horacio B; Parborell, Fernanda; Owen, Gareth I

    2013-05-01

    Reproductive success stems from a finely regulated balance between follicular maturation and atresia, in which the role of carbohydrate structure is poorly understood. Here, we describe for the first time a fraction of purified recombinant human FSH that is capable of bringing about the cell death of granulosa cells and preventing follicular maturation in a rat model. Further analysis by mass spectrometry revealed the presence of the lectin Concanavalin-A (Con-A) within this fraction of recombinant FSH. Using both the fractionated FSH and Con-A, the observed cell death was predominantly located to the granulosa cells. Ex vivo culture of rat follicles demonstrated that follicle degeneration occurred and resulted in the release of a denuded and deteriorated oocyte. Moreover, in vivo experiments confirmed an increase in atresia and a corresponding reduction confined to follicle in early antral stage. As a mechanism of action, Con-A reduces ovarian proliferation, Von Willebrand staining, and angiogenesis. Based on the observation that Con-A may induce granulosa cell death followed by follicle death, our results further demonstrate that follicular carbohydrate moiety is changing under the influence of FSH, which may allow a carbohydrate-binding lectin to increase granulosa cell death. The physiological consequences of circulating lectin-like molecules remain to be determined. However, our results suggest a potential exploitation of carbohydrate binding in fertility and ovarian cancer treatment. This work may shed light on a key role of carbohydrates in the still obscure physiological process of follicular selection and atresia.

  16. Genome-wide analysis shows no genomic predictors of ovarian response to stimulation by exogenous FSH for IVF

    NARCIS (Netherlands)

    van Disseldorp, Jeroen; Franke, Lude; Eijkemans, Rene; Broekmans, Frank; Macklon, Nick; Wijmenga, Cisca; Fauser, Bart

    The current proof of principle study explores the possibility that a genetic single-nucleotide polymorphism (SNP) profile is associated with ovarian response to standardized stimulation for IVF using exogenous FSH. Such a pharmacogenomic approach could aid in rendering ovarian stimulation for IVF

  17. Blocking antibody to the beta-subunit of FSH prevents bone loss by inhibiting bone resorption and stimulating bone synthesis

    Science.gov (United States)

    Low estrogen levels undoubtedly underlie menopausal bone thinning. However, rapid and profuse bone loss begins three years prior to the last menstrual period, when serum estrogen is relatively normal. We have shown that the pituitary hormone FSH, the levels of which are high during the late peri-men...

  18. Serum FSH levels and testicular histology in infertile men with non obstructive azoospermia and Y chromosome microdeletions

    Directory of Open Access Journals (Sweden)

    Rajeev Kumar

    2006-01-01

    Full Text Available Objectives: Men with nonobstructive azoospermia may father a child through intracytoplasmic injection of testicular sperms. This can result in transmission of genetic defects such as Y chromosome microdeletions which primarily caused the infertility and occur in up to 55% such men. It may not be feasible to screen all patients for Y chromosome microdeletions due to the cost and technical difficulty. A correlation with existing markers such as follicle stimulating hormone (FSH and testicular histology may help identify a subgroup for screening. We therefore studied the association between regions of azoospermia factor (AZF deletion, testicular histology and serum FSH level in men with nonobstructive azoospermia. Materials and Methods: One hundred and nine men presenting with primary infertility and diagnosed as nonobstructive azoospermia based on standard guidelines were included in the study. Fasting blood sugar, serum FSH, testosterone and prolactin estimation was done and testicular fine needle aspiration biopsy was performed where clinically indicated. Patients with normal karyotype on standard Q banding (n=82 were evaluated for microdeletions in the Y chromosome. Eight AZF loci which mapped to interval 5 and 6 of the Y chromosome were evaluated. Results: Microdeletions were found in seven of 82 men with normal karyotype (8.5%. Three patients had deletions in both AZFa and AZFb regions with Sertoli cell only (SCO histology. Two had AZFc deletion with hypospermatogenesis and maturation arrest in one each. Two patients had cryptorchidism. FSH levels were higher (mean 38.77 mIU/ml in patients with deletions in the AZFa and AZFb regions than in those with AZFc deletions (mean 5.86 mIU/ml. In patients without a deletion, FSH was higher in the group with SCO (mean 18.28 mIU/ml compared to those with hypospermatogenesis or maturation arrest (mean 6.83 mIU/ml. Conclusions: Serum FSH is raised in patients with severely depleted germ cell function

  19. Effect of season and mating system in Awassi ewes superovulated with FSH on fertilization rate and embryo recovery

    Directory of Open Access Journals (Sweden)

    M. K. M. A. Al-Mola

    2010-01-01

    Full Text Available The aim of the present study was to evaluate the contribution of laparoscopic intrauterine insemination to the improvement of fertilization and embryo recovery in Awassi ewes superovulated with FSH in breeding and non-breeding season. Twelve nonpregnant and cycling Awassi ewes of 3-4 years of age were randomly allocated in equal numbers (n = 6 to two groups. Each ewe was treated with a progesterone impregnated intravaginal sponge for 12 days. All ewes were superovulated with FSH in eight reducing doses for four days in the morning and evening from two days prior to sponge withdrawal. Ewes of group 1 were mated naturally at least two times with Awassi rams of proven fertility. Ewes of group 2 had intrauterine insemination and were conducted 44-46 h after sponge removal, under laparoscopic visualization of uterine horns, depositing 1 ml of semen in the distal portion of each uterine horn. Ovarian response was assessed by determining number of corpora lutea by laparoscopy on day 6 after mating. Embryo recovery was performed by hand assisted laparoscopy and by flushing both uterine horns. Ovarian response of the ewes superovulated with FSH was decreased to half in the non-breeding season. High number of unfertilized ova (P<0.05 was observed in ewes superovulated with FSH in the non-breeding season when naturally inseminated compared to ewes inseminated intrauterine using laparoscopic technique. Higher rates of embryo recovery (P<0.05 were achieved in superovulated ewes in the breeding season when ewes were inseminated by laparoscopic intrauterine insemination. The fertilization rates in ewes inseminated intrauterine using laparoscopic techniques in breeding season and non-breeding season were 88.1% and 37.98%, respectively. It could be concluded from the results of the present study that the use of FSH to induce superovulation in Awassi ewes combined with laparoscopic intrauterine insemination can increase the fertilization rate in the breeding

  20. Analysis of ovarian reserve markers (AMH, FSH, AFC) in different age strata in IVF/ICSI patients

    Science.gov (United States)

    Shahrokh Tehraninezhad, Ensieh; Mehrabi, Fatemeh; Taati, Raheleh; Kalantar, Vahid; Aziminekoo, Elham; Tarafdari, Azam

    2016-01-01

    Background: The predictive roles of follicle stimulating hormone (FSH), anti-mullerian hormone (AMH) and antral follicle count (AFC) as ovarian reserve markers in women with different age groups are not established well. Objective: This study compares the value of FSH, AMH and AFC at the time of in vitro fertilization (IVF) treatment in different age groups. Materials and Methods: In this cross-sectional study, 103 women aged 20-43 years candidates for IVF/ICSI cycle were recruited. FSH, AMH and AFC on day 3 of menstrual cycle were measured. The relationship of these measured markers with outcome variables (oocytes number, number of frozen/fresh embryo and chemical and clinical pregnancy) was assessed in different age groups (i.e. 20-32, 33-37 and 38-43 years). Results: our results show that age was correlated with clinical pregnancy, oocyte count and fresh and frozen embryo (p<0.001). AMH, AFC and FSH were not correlated with clinical or chemical pregnancy at total population or age subgroups except the significant correlation of AFC with clinical pregnancy at 33-37 years old group. AFC was correlated with oocyte count and the number of fresh and frozen embryos in the ages group 20-32 years. In this age group, AMH was correlated with fresh and frozen embryos. AMH, AFC and FSH were correlated with oocyte count and the number of fresh embryos in age group 33-37 years. AMH was correlated with oocyte count and the number of fresh embryos in 38-43 years old group. Conclusion: We concluded that the age is the superior predictor of IVF outcome and AMH and AFC are variable predicting markers of ovarian reserve in different age groups. PMID:27679824

  1. Comparison of Sensitivity of FSH Levels and Vaginal pH in Diagnosis of Menopause, Estradiol Levels and Menopausal Signs

    Directory of Open Access Journals (Sweden)

    N Zafarqandi

    2011-05-01

    Full Text Available Introduction: As immunoassay and Eliza are used nowadays for checking levels of FSH and estradiol, the role of vaginal acidity as a sign of responding vaginal cells to estrogen has been forgotten, although its role in this field is known. The purpose of this study was to assess the sensitivity of vaginal pH in diagnosis of menopause, estradiol levels, hot flashes and vaginal dryness that are frequent problems. Methods: This descriptive – cross sectional study included 250 women aged 45- 60 years. Population sampling was nonrandom and inclusion factors included absence of hormonal therapy, absence of vaginal douche or coitus during the past three days. Vaginal pH, FSH and estradiol levels were determined. The analysis was carried out using SPSS. For comparing two variables, Spearman, Pearson and chi-square tests were used. Results: The mean age of the population sample was 50.4 years(SD ± 4.4 of which 45.7% were menopause and 54.3% were in menopausal transition. Mean vaginal pH was 5.57±0.66. The association between vaginal pH, FSH and estradiol was significant (P=0.047, P=0.000. In this study, the cut off point of FSH for diagnosing menopause was ≥20 mu/ml. The sensitivity of vaginal pH>5 with this level of FSH was determined to be 60%. Sensitivity of vaginal pH>5 in diagnosing estradiol ≤40pg/ml was determined to be 55%. The association between hot flashes and vaginal dryness with vaginal acidity was significant. Conclusion: Vaginal pH is an easy, noninvasive, alternative and cheap assay for diagnosing menopause, estradiol levels and menopausal signs.

  2. Efficacy of highly purified urinary FSH versus recombinant FSH in elder Chinese women undergoing assisted reproductive techniques%高纯尿源性卵泡刺激素在高龄妇女控制性超排卵中的临床应用

    Institute of Scientific and Technical Information of China (English)

    刘雪梅; 郝翠芳; 王金芳; 张宁; 曲庆兰; 包洪初

    2013-01-01

    To determine which FSH, urinary or recombinant, has better efficacy in elder Chinese women undergoing assisted reproductive techniques(ART).Methods: A total of 508 patients over 37 years were randomized into two groups, 254 cycles for each group. 254 patients were treated with recombinant FSH,and another 254 patients with urinary FSH. The patients in both groups were down-regulated by the gonadotrophin-releasing hormone (GnRH) analogue long protocol. The main outcomes including days of stimulation,serum E2 level on the HCG day,number of oocytes collected, total amount of FSH, ratio of FSH/oocyte, MⅡ oocyte rate,2PN zygote rate, grade 1 embryo rate, pregnancy rate, implantation rate, miscarriage rate and cycle cancellation rate were compared between the two groups. Results:Twenty two cycles including 16 cycles with poor ovarian response and six cycles with varian hyperstimulation syndrome were cancelled. There were 243 cycles left in each group. The patients treated with urinary FSH had a significantly higher 2PN zygote rate(87. 4% vs. 76. 6%, P0.05). However, lower miscarriage rate(23. 5% vs. 29. 1%)(although not significant)in urinary FSH group might be noteworthy. Conclusions: Our study showed that urinary FSH performed better in aged Chinese women than recombinant FSH,although both kinds of FSHs produced roughly equivalent pregnancy outcomes.

  3. Habitat monitoring plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Management of habitat is required in order to achieve the refuge purpose and wildlife objectives. The Upland Habitat Management Plan (1993, Interim Plan) and the...

  4. EcologicHabitat_WCV

    Data.gov (United States)

    Vermont Center for Geographic Information — WCV describes the value of the Wildlife Habitat Suitability as it approaches the state highway system. This analysis was designed to use the Wildlife Habitat...

  5. California Condor Critical Habitat

    Data.gov (United States)

    California Department of Resources — These Data identify (in general) the areas where critical habitat for the California Condor occur. Critical habitat for the species consists of the following 10...

  6. Predictive Seagrass Habitat Model

    Science.gov (United States)

    Restoration of ecosystem services provided by seagrass habitats in estuaries requires a firm understanding of the modes of action of multiple interacting stressors including nutrients, climate change, coastal land-use change, and habitat modification. We explored the application...

  7. Recombinant LH supplementation to recombinant FSH during the final days of controlled ovarian stimulation for in vitro fertilization. A multicentre, prospective, randomized, controlled trial

    DEFF Research Database (Denmark)

    Nyboeandersen, A; Al Humaidan, Peter Samir Heskjær; Fried, G

    2008-01-01

    either rFSH (Gonal-F) alone (n = 261) or to continue after Day 6 of stimulation with both rFSH (Gonal-F) and rLH (Luveris) (n = 265) from Day 6. The starting dose of rFSH was 150-225 IU/day according to age below or above 35 years. RESULTS: Ongoing pregnancy rate at week 10-12 was 28.7% after rFSH alone...... and 27.2% after rFSH + rLH. This showed no evidence of a difference. Administration of rLH significantly (P....9% low Day 1 LH; 22.1% low Day 6 LH) versus rFSH + rLH (25.0% low Day 1 LH; 28.9% low Day 6 LH). CONCLUSIONS: Supplementing rFSH with daily doses of 75-150 IU of rLH during the second half of the follicular phase showed no evidence of increasing the ongoing pregnancy rates in the general population...

  8. Repeated superovulation using a simplified FSH/eCG treatment for in vivo embryo production in sheep.

    Science.gov (United States)

    Forcada, F; Ait Amer-Meziane, M; Abecia, J A; Maurel, M C; Cebrián-Pérez, J A; Muiño-Blanco, T; Asenjo, B; Vázquez, M I; Casao, A

    2011-03-01

    This study investigated the efficacy of a simplified repeated superovulation treatment (eCG plus FSH in a single dose, rather than the usual protocol of six decreasing doses of FSH) in the in vivo embryo production in Ojalada donor ewes during the breeding season. In vitro viability after vitrification and warming of embryos recovered from both treatments was also assessed. In addition, the study examined the effects of the concentration of anti-eCG antibodies before each eCG/FSH treatment on in vivo embryo production. Thirty-eight females at the end of their reproductive lives were given the decreasing (n = 19) or simplified (n = 19) superovulatory treatment up to three times at intervals of ≥ 50 d. The onset of estrus was 5 h earlier (P < 0.05) among ewes that received the eCG/FSH protocol (25.2 ± 0.80 h) than it was among those that received the decreasing superovulatory treatment (30.1 ± 1.0 h), but the two treatments did not differ significantly in ovulation rates or the number and viability of embryos recovered. Both of the superovulatory protocols were significantly (P < 0.05 to P < 0.01) less effective after the first application. After three superovulatory treatments, the average number of viable embryos per ewe was 14.1 ± 2.3 and 13.7 ± 2.5 in the decreasing and simplified protocols, respectively. High anti-eCG antibody concentrations just before the superovulatory treatment with eCG/FSH were associated with a significant decrease (P < 0.05) in the rates of fertilization, viability, and freezability, especially in the second and third recoveries. Repeated superovulatory treatments with eCG/FSH can provide an efficient means of producing high quality embryos in the ewes of endangered breeds at the end of their reproductive lives, although further studies are needed to characterize the response associated with high concentrations of anti-eCG antibodies. Copyright © 2011 Elsevier Inc. All rights reserved.

  9. Screening of FSH receptor gene in Argentine women with premature ovarian failure (POF).

    Science.gov (United States)

    Sundblad, Victoria; Chiauzzi, Violeta A; Escobar, Maria Eugenia; Dain, Liliana; Charreau, Eduardo H

    2004-07-30

    Diverse mutations in FSH-receptor (FSHR) gene have been described as possible cause of premature ovarian failure (POF). To investigate the presence of mutations and/or polymorphisms in FSHR gene, DNA from 20 POF, 5 of which were diagnosed as resistant ovary syndrome (ROS), and from 44 controls was isolated from peripheral lymphocytes. The complete coding sequence was analysed by PCR followed by SSCP, direct sequencing or restriction enzyme analysis. No mutations in FSHR gene were identified in the patients studied. The two already described polymorphisms in exon 10, A919G and A2039G, cosegregated in all the homozygous individuals, indicating that FSHR presents two isoforms: Ala307-Ser680 and Thr307-Asn680. OR results suggest that the 919G-2039G allelic variant or the homozygous genotype is not associated to disease risk. In addition, a heterozygous substitution T1022C (Val341Ala) was found in two control subjects. We suggest that mutations in FSHR gene are rare in women with POF in Argentine. Presence of a particular FSHR isoform does not appear to be associated with this disease.

  10. Pueraria tuberosa DC Extract Improves Androgenesis and Sexual Behavior via FSH LH Cascade

    Directory of Open Access Journals (Sweden)

    Nagendra Singh Chauhan

    2013-01-01

    Full Text Available The aim of this study was to investigate the effects of ethanolic extract of Pueraria tuberosa (PT on sexual behaviour and androgenic activity. Male albino rats were divided into four groups of six animals each: control group 1 (2% acacia solution, PT-treated group 2 (50 mg/Kg, PT-treated group 3 (100 mg/Kg, and PT-treated group 4 (150 mg/Kg. Sexual behavior of male rats in the presence of a female rat was recorded. The treated groups were evaluated for sexual parameters. The extract was characterized using LC-MS. The effect of treatment on anabolic and weight of secondary sexual organs was determined. The histological changes in section of testis and epididymis after treatment were observed. Sperm count in epididymis and fructose content in seminal vesicles were also measured. Levels of hormones like FSH, LH, and T were determined. A dose-dependent increase in sexual behaviors was evidenced in the animals of extract treated groups. Increase in testis weight was recorded in PT. At the highest dose PT also affects the hormones level. The four compounds namely puerarin, daidzein, biochanin-A and formononetin were identified in ethanolic extract using LC-MS. It concluded that PT extract possesses androgenic effect and it significantly increased the sexual behaviour and hormones level.

  11. Intrafollicular Endocrine Milieu After Addition of hCG to Recombinant FSH During Controlled Ovarian Stimulation for In Vitro Fertilization

    DEFF Research Database (Denmark)

    Thuesen, L L; Andersen, A Nyboe; Loft, A;

    2014-01-01

    CONTEXT: The role of human chorionic gonadotropin (hCG) supplementation on the intrafollicular steroid milieu has been studied. OBJECTIVE: The objective of the study was to assess the impact on steroid levels in follicular fluids (FFs) after different doses of hCG supplementation to recombinant FSH...... protocol with recombinant FSH 150 IU/d and randomized from stimulation day 1 to supplementation with hCG: D0, 0 IU/d; D50, 50 IU/d; D100, 100 IU/d; and D150, 150 IU/d. MAIN OUTCOME MEASURE: Intrafollicular hormone concentrations in relation to treatment groups, follicular sizes, and embryo quality were...... measured. RESULTS: In large follicles, hCG supplementation induced a nearly 3-fold increase of estradiol (nanomoles per liter) [D0: 1496; D50: 3138; D100: 4338; D150: 4009 (P

  12. Photoperiod-gonadotropin mismatches induced by treatment with acyline or FSH in Siberian hamsters: impacts on ovarian structure and function.

    Science.gov (United States)

    Zysling, D A; Park, S-U; McMillan, E L; Place, N J

    2012-11-01

    Many seasonal breeders time their reproductive efforts to specific times of the year to ensure adequate resources for the production and care of young. For long-day (LD) breeders, females born before the summer solstice (LDs) reach sexual maturity quickly and often breed that same year, whereas females born after the summer solstice (short days (SDs)) may delay reproductive development to the following spring when environmental conditions are favorable for reproduction. In Siberian hamsters, development in SD is associated with structural and functional differences in the ovary compared with females held in LD, including a greater number of primordial follicles and an abundance of hypertrophied granulosa cells (HGCs), which are immunoreactive for anti-Müllerian hormone. The goal of this study was to determine whether SD-induced gonadotropin suppression is responsible for these phenotypic differences. Gonadotropin levels were suppressed in LD hamsters using the GNRH antagonist acyline. Conversely, to determine whether the SD ovarian phenotype is completely reversed by gonadotropin stimulation, recombinant human FSH (rhFSH) was administered. Our treatments were successful in mimicking FSH concentrations of the opposite photoperiod, but they did not produce a comparable change in the ovarian phenotype. Most notable was the lack of HGCs in the ovaries of acyline-treated LD females. Similarly, HGCs were maintained in the ovaries of SD females treated with rhFSH. Our data suggest that gonadotropins alone do not account for the SD ovarian phenotype. Future studies will determine whether SD-induced changes in other factors underlie these phenotypic changes.

  13. Neonatal androgenization of hypogonadal (hpg male mice does not abolish estradiol-induced FSH production and spermatogenesis

    Directory of Open Access Journals (Sweden)

    Kerr Jeffrey B

    2005-09-01

    Full Text Available Abstract Background Testicular development is arrested in the hypogonadal (hpg mouse due to a congenital deficiency in hypothalamic gonadotropin-releasing hormone (GnRH synthesis. Chronic treatment of male hpg mice with estradiol induces FSH synthesis and secretion, and causes testicular maturation and qualitatively normal spermatogenesis. As estradiol negative feedback normally inhibits FSH production in the male, this study tested whether this paradoxical response to estradiol in the male hpg mouse might be due to inadequate masculinisation or incomplete defeminization in the neonatal period. Previous studies have demonstrated that treatment of hpg mice with testosterone propionate in the immediate neonatal period is necessary to allow full reproductive behaviors to be expressed following suitable endocrine stimulation at adult ages. Methods Hpg mice were treated with 100 μg testosterone propionate or vehicle on postnatal day 2. At 35 days of age, subgroups of these mice were treated with silastic implants containing estradiol or cholesterol. Reproductive behavior was scored in tests with steroid-primed female mice, then testicular development was assessed histologically, and measures of pituitary FSH content made at 85 days of age. Results The neonatal testosterone propionate treatment successfully defeminized female litter mates, as revealed by impaired vaginal opening and deficiencies in lordosis behavior, and it allowed appropriate male reproductive behavior to be expressed in a proportion of the hpg males when tested at an adult age. However, neonatal androgen supplementation did not block or even reduce the subsequent actions of estradiol in increasing pituitary FSH content, nor did it affect the ability of estradiol to induce qualitatively normal spermatogenesis. Conclusion The ability of the hpg male to show a "female" neuroendocrine response to estradiol is not a result of inadequate androgenization during neonatal development, and

  14. FSH Injections and Ultrasonography Determine Presence of Ovarian Components in the Evaluation of Ovotesticular Disorders of Sex Development

    Directory of Open Access Journals (Sweden)

    Shannon French

    2009-01-01

    Full Text Available Three infants with ambiguous genitalia and suspected ovotestes were given recombinant FSH to induce ovarian follicular development. The development of follicles in the gonadal tissue suggested the presence of ovarian tissue in two of the three infants. This method may provide a means to better characterize gonadal anatomy in patients affected by disorders of sex development (DSD. Sonographic information poststimulation provided parents with earlier and more specific education and support concerning the possible need for confirmative gonadal biopsy treatment options.

  15. FSH Injections and Ultrasonography Determine Presence of Ovarian Components in the Evaluation of Ovotesticular Disorders of Sex Development

    Directory of Open Access Journals (Sweden)

    Hicks John

    2009-12-01

    Full Text Available Three infants with ambiguous genitalia and suspected ovotestes were given recombinant FSH to induce ovarian follicular development. The development of follicles in the gonadal tissue suggested the presence of ovarian tissue in two of the three infants. This method may provide a means to better characterize gonadal anatomy in patients affected by disorders of sex development (DSD. Sonographic information poststimulation provided parents with earlier and more specific education and support concerning the possible need for confirmative gonadal biopsy treatment options.

  16. Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats

    DEFF Research Database (Denmark)

    Jensen, Anna Guldvang; Hansen, Cecilie Hurup; Weisser, Johan J;

    2015-01-01

    .3 (L), 5.0 (M), and 20.0 (H)mg SV/kg bw/day for 14 days. 10 Steroids were investigated in H295R growth media, and in tissues and plasma from rats using GC-MS/MS. Plasma LH and FSH were quantified by ELISA. In the H295R assay, SV and SVA particularly decreased progestagens with IC50-values from 0...

  17. Differential Expression of Claudin Family Proteins in Mouse Ovarian Serous Papillary Epithelial Adenoma in Aging FSH Receptor-Deficient Mutants

    Directory of Open Access Journals (Sweden)

    Jayaprakash Aravindakshan

    2006-12-01

    Full Text Available Ovarian cancer is a deadly disease with long latency. To understand the consequences of loss of folliclestimulating hormone receptor (FSH-R signaling and to explore why the atrophic and anovulatory ovaries of follitropin receptor knockout (FORKO mice develop different types of ovarian tumors, including serous papillary epithelial adenoma later in life, we used mRNA expression profiling to gain a comprehensive view of misregulated genes. Using real-time quantitative reverse transcription-polymerase chain reaction, protein analysis, and cellular localization, we show, for the first time, in vivo evidence that, in the absence of FSH-R signaling, claudin-3, claudin-4, and claudin-11 are selectively upregulated, whereas claudin-1 decreases in ovarian surface epithelium and tumors in comparison to wild type. In vitro experiments using a mouse ovarian surface epithelial cell line derived from wild-type females reveal direct hormonal influence on claudin proteins. Although recent studies suggest that cell junction proteins are differentially expressed in ovarian tumors in women, the etiology of such changes remains unclear. Our results suggest an altered hormonal environment resulting from FSH-R loss as a cause of early changes in tight junction proteins that predispose the ovary to late-onset tumors that occur with aging. More importantly, this study identifies claudin-11 overexpression in mouse ovarian serous cystadenoma.

  18. Induction of spermatogenesis by rhFSH for azoospermia due to spermatogenic dysfunction with maturation arrest: five case series.

    Science.gov (United States)

    Kobori, Yoshitomo; Suzuki, Keisuke; Iwahata, Toshiyuki; Shin, Takeshi; Sato, Ryo; Nishio, Kojiro; Yagi, Hiroshi; Arai, Gaku; Soh, Shigehiro; Okada, Hiroshi

    2015-06-01

    When sperm cannot be retrieved from the testes of patients with azoospermia due to spermatogenic dysfunction (ASD), there is no rational way for the patient to become a biological father. We investigated the possibility of inducing spermatogenesis in such patients by hormonal therapy with recombinant human follicle-stimulating hormone (rhFSH) alone. Twenty-six ASD patients who could not obtain spermatozoa by microdissection testicular sperm extraction (micro-TESE) were confirmed to have arrested spermatogenesis at the late stage of maturation arrest. They were subsequently treated with 75-150 IU two times/week rhFSH alone for 12 months. The primary endpoint was the appearance of sperm in ejaculate, and we followed the patients to determine the outcome of inseminating their partners. After rhFSH treatment, mature spermatozoa were found in the ejaculate in five of 26 (19.2%) patients, all of whom showed histology of non-uniform type maturation arrest. Intracytoplasmic sperm injection of the mature spermatozoa resulted in two ongoing clinical pregnancies (insemination success rate, 40.0%). Recombinant human follicle-stimulating hormone treatment can be used as an advanced assisted reproductive technology to improve spermatogenesis in some azoospermic patients with maturation arrest of spermatogenesis and is a potential treatment option after unsuccessful micro-TESE.

  19. The Effects of Trifluralin on LH, FSH and Testosterone Hormone Levels and Testis Histological Changes in Adult Rats

    Directory of Open Access Journals (Sweden)

    Mehrdad Shariati

    2008-01-01

    Full Text Available Background: Trifluralin is a herbicide and used in agriculture widely. It enters plants throughdeveloping roots and stops plant cells from division and elongation (meristemic inhibitor.Extensive application of trifluralin to control annual grasses and broadleaf weeds in agriculture,horticulture and horn garden, leads to environmental pollution and its entrance into the food chaincould have determined effects on human and other species. In this research the effects of trifluralinon reproductive parameters of the male rats including serum luteinizing hormone (LH, folliclestimulatinghormone (FSH, testosterone levels, and changes in testicular tissue and body weightwere investigated.Materials and Methods: For this purpose male rats were randomly divided in 5 groups, to includecontrol, sham (received normal saline as a solvent, and three experimental groups which received500, 1000 and 2000 mg/kg oral trifluralin respectively. After 16 days, body and testis weight weremeasured and blood samples were taken from heart and used for measurement of LH, FSH andtestosterone levels. To evaluate histological changes, testes were removed and weighed and, afterobtaining tissue section, stained by HE.Results: Serum testosterone, FSH, and LH levels showed significant decrease in experimentalgroups (p<0.05. There was significant decrease in the number of germinal and somatic cells intestis in experimental groups. There was also a significant decrease in body and testis weight inexperimental groups.Conclusion: It can be concluded that oral administration of trifluralin could decrease gonadotropinsand testosterone hormone levels and also this herbicide could have hazardous effects on testistissue.

  20. Follicular and endocrine dose responses according to anti-Müllerian hormone levels in IVF patients treated with a novel human recombinant FSH (FE 999049)

    DEFF Research Database (Denmark)

    Bosch, Ernesto; Nyboe Andersen, Anders; Barri, Pedro

    2015-01-01

    responses were observed for the number of follicles ≥ 12 mm, and serum levels of oestradiol, inhibin B, inhibin A and progesterone at end of stimulation. In comparison with the higher AMH stratum, patients in the lower AMH stratum had significantly different slopes of the dose-response curves...... for these hormones, and no clear dose-related increase was observed for the number of follicles in these patients. CONCLUSIONS: Dose-response relationships between rhFSH and follicular development and endocrine parameters are significantly different for IVF/ICSI patients with lower and higher serum AMH levels......: Follicular development and endocrine parameters during controlled ovarian stimulation (COS) with rhFSH. RESULTS: Serum FSH levels increased with increasing rhFSH doses and steady-state levels for each dose were similar in both AMH strata. In the whole study population, significant (P dose...

  1. Trends on Habitat Management

    Directory of Open Access Journals (Sweden)

    Raluca Giuşcă

    2008-01-01

    Full Text Available According to traditional image, human habitat constitution is the result of natural inter-relations, the fundamental premise of the existence of natural resources, the climate, and the access to more developed proximities for commercial trading. Human habitat represents a complex system, with environmental values, having live and natural components that are inter-related. The dwelling is the fundamental component of the habitat and by relationship with the other components determines the level of habitation.

  2. Shorebird Habitat Suitability Indicies

    Data.gov (United States)

    Arctic Landscape Conservation Cooperative — This dataset consists of predicted habitat suitability indices and species richness for eight shorebird species (Black-bellied Plover [Pluvialis squatarola],...

  3. Endocrine effects of hCG supplementation to recombinant FSH throughout controlled ovarian stimulation for IVF: a dose–response study

    DEFF Research Database (Denmark)

    Thuesen, Leif; Smitz, J; Loft, A;

    2013-01-01

    To analyse the endocrine response in relation to the Δ-4 and Δ-5 pathways of ovarian steroidogenesis after different doses of human chorionic gonadotrophin (hCG) supplementation to recombinant FSH from Day 1 of controlled ovarian stimulation for IVF.......To analyse the endocrine response in relation to the Δ-4 and Δ-5 pathways of ovarian steroidogenesis after different doses of human chorionic gonadotrophin (hCG) supplementation to recombinant FSH from Day 1 of controlled ovarian stimulation for IVF....

  4. Pre- and postoperative status of gonadotropins (FSH and LH) and inhibin-B in relation to testicular histopathology at orchiopexy in infant boys with unilateral undescended testes

    DEFF Research Database (Denmark)

    Thorup, Jørgen Mogens; Clasen-Linde, Erik; Thorup, Sebastian Cortes

    2015-01-01

    had preoperatively elevated serum levels of both LH 0.5-2.3 IU/l (mean: 1.0 IU/l) and FSH 1.3-2.2 IU/l (mean: 1.6 IU/l). Eight of these patients normalized both LH and FSH serum values postoperatively. None of the 4 boys with impaired inhibin-B level that did not normalize after surgery had elevated...

  5. HUMAN FOLLICLE STIMULATING HORMONE (hFSH AND THYROXINE (T4 IN SURVIVAL MAINTENANCE AND IN VITRO GROWTH PROMOTION OF CAPRINE PREANTRAL FOLLICLES

    Directory of Open Access Journals (Sweden)

    Sanely Lourenço da Costa

    2015-04-01

    Full Text Available The aim of this study was to investigate the interaction of human FSH (10ng/ml with T4 (20ng/mL on survival, activation and growth of preantral follicles cultured in vitro for 28 days. Fragments of non-cultured and cultured ovarian tissue were processed for classic histology and transmission electron microscopy. The results showed a reduction in the survival rate in all the media tested (one to 28 days when compared to the fresh control. However the treatment with T4/hFSH for seven days of culture maintained the rate similar to the control. The media tested by one and 28 days reduced the percentage of primordial follicles in all periods of culture. However, T4/hFSH on day one of culture remained similar to the fresh control. None of the media were able to keep the percentage of the developing follicles. It was observed that the follicular diameter in the medium with T4/hFSH remained similar to the fresh control. The ultrastructural analysis confirmed the integrity of follicles cultured for seven days in a medium supplemented with T4/hFSH. In conclusion, the medium with T4/hFSH is able to maintain the survival, promote the activation, and the ultrastructural integrity of caprine preantral follicles for until seven days.

  6. Inlfuence of FSH Treatment on Expression of CDC25A, TSSK3 and P53in Vitro Cultured Sertoli Cells of Calf

    Institute of Scientific and Technical Information of China (English)

    Li Yu-long; Wu Qiong; Zhao Xun-wu; Zeng Yue; Elkanah Adegoke; Zhang Gui-xue

    2015-01-01

    CDC25A, TSSK3 and P53 expressionsin vitro in cultured sertoli cells after FSH treatment were studied in order to provide some data for further researches of spermatogenesis. Different concentrations of FSH (0, 0.01, 0.02, 0.04, and 0.08 IU• mL-1) were used to treat sertoli cells culturedin vitro. The expression of CDC25A, TSSK3 and P53 was determined by real-time-PCR at 6 h, 12 h and 24 h after FSH treatment of sertoli cells. The results showed that FSH had no significant effect on expression of CDC25A (p>0.05), could significantly improve the expression of TSSK3 and P53 (p<0.05), and had no significant effect on expression of CDC25A in sertoli cells, but it could significantly improve the expression of TSSK3. CDC25A was likely to play a role in other signaling pathways in sertoli cells. Within the range of certain concentration of FSH, TSSK3 in sertoli cells had the highest expression at about 24 h. TSSK3 protein produced in sertoli cells was likely to play an important role in substrate-level phosphorylationbe in meiosis and mitosis of spermatogenic cells. FSH could promote P53 expression and the highest expression was at about 12 h, and P53 might control the division of spermatogenic cells as well as sertoli cells.

  7. Diferentes origens do Hormônio Folículo Estimulante (FSH influenciam a viabilidade e o desenvolvimento de folículos pré-antrais caprinos

    Directory of Open Access Journals (Sweden)

    Deborah de Melo Magalhães

    2009-10-01

    Full Text Available The aim of this study was to evaluate the effects of pituitary (pFSH or recombinant (rFSH FSH on the survival and growth of caprine preantral follicles. Caprine ovarian tissues were in vitro cultured for one or seven days in Minimum Essential Medium (MEM alone or containing 10, 50, 100 and 1000 ng/ml of pFSH or rFSH. Control tissues (non-cultured and those cultured were processed for histological and ultrastructural studies. In addition, follicular and oocyte diameter were analysed. After seven days of culture, only 50 ng/ml of rFSH maintained the percentage of normal follicles similar to control. Moreover, 10 ng/ml of pFSH and all the concentrations of rFSH promoted primordial follicles activation. In addition, the presence of 50 ng/ml of rFSH promoted the highest follicular diameter at day seven of culture. In conclusion, 50 ng/ml of rFSH maintained the ultrastructural integrity of caprine preantral follicles, promoted primordial follicles activation and further growth of cultured follicles.

  8. Effect of three pFSH doses on superovulation and embryo quality in goats during two breeding seasons in north-eastern mexico.

    Science.gov (United States)

    Sánchez-Dávila, F; Ledezma-Torres, R A; Padilla-Rivas, G; Del Bosque-González, A S; González Gómez, A; Bernal-Barragán, H

    2014-08-01

    The aim of this research was to evaluate the effectiveness of three pFSH doses (80 mg; 145 mg and 215 mg) on ovarian response and on quantity and quality of transferable embryos of goats during the breeding and the non-breeding seasons. Ovary structures were exposed (laparatomy under general anaesthesia) and numbers of follicles and corpora lutea were registered. Surgical embryo flushing was conducted to count and classify embryos. There were more follicles (3.4 ± 1.1) in does administered 80 mg of pFSH (p < 0.05) than in goats administered 145 mg of pFSH (2.2 ± 1.1) and 215 mg of pFSH (0.9 ± 0.6). Numbers of corpora lutea, blastocysts, and recovered and transferable embryos of goats administered 145 mg pFSH (13.4 ± 3.7, 2.42 ± 1.0, 3.4 ± 1.2 and 3.2 ± 1.1, respectively) and those of goats administered 215 mg pFSH (11.6 ± 2.6, 3.2 ± 0.9, 5.7 ± 1.5, and 5.6 ± 1.5) were greater (p < 0.05) than values obtained from goats administered 80 mg pFSH (4.0 ± 1.5, 0.5 ± 0.3, 1.0 ± 0.5, and 0.8 ± 0.5). Numbers of morula of does administered 80 and 145 mg pFSH (0.4 ± 0.4 and 0.8 ± 0.3) were lower (p < 0.05) than those obtained from animals treated with 215 mg pFSH (2.4 ± 0.9). There was no effect of season upon the analyzed variables. In conclusion, under the prevalent conditions in north-eastern Mexico, administration of 145 or 215 mg pFSH in a decreasing dose schedule over 3.5 days to bred goats provided a satisfactory superovulatory result. © 2014 Blackwell Verlag GmbH.

  9. Habitat Blocks and Wildlife Corridors

    Data.gov (United States)

    Vermont Center for Geographic Information — Habitat blocks are areas of contiguous forest and other natural habitats that are unfragmented by roads, development, or agriculture. Vermonts habitat blocks are...

  10. Immunodetection of Luteinizing Hormone (LH, Follicle-Stimulating Hormone (FSH, Thyroid Stimulating Hormone (TSH and Prolactin (PRL in Brachionus calyciflorus (Rotifera: Monogononta

    Directory of Open Access Journals (Sweden)

    Jesús Alvarado-Flores

    2009-12-01

    Full Text Available The endocrine system controls and coordinates behavioral, biochemical, and physiological processes through signal mechanisms using neuropeptides or products of neurosecretory cells. Among invertebrates, this system is poorly studied in rotifers, in which estrogens and androgens significantly affect sexual reproduction. This is the first report of the presence of the Luteinizing Hormone (LH, Follicle-Stimulating Hormone (FSH, Thyroid Stimulating Hormone (TSH and Prolactin (PRL in rotifers. Analyses included the avidin-biotin-peroxidase complex method with primary antibodies LH (Anti-Rat LH serum for RIA, PRL (Anti-Rat PRL serum for RIA, FSH (Anti-Rat FSH serum for RIA and TSH (Anti-Rat TSH serum for RIA. These hormones were found in females, males and parthenogenetic and sexual eggs of the freshwater Brachionus calyciflorus. The immunoreactivity of FSH, LH, TSH and PRL in females was observed in: ovaries, cerebrum, mastax, stomach, lorica, and the stomach gland. However, in males LH was observed only at the trochal disk and cerebrum. The hormones FSH, TSH and PRL, were observed in testicles, contractil vesicles, and cementary gland of males. Regarding amictic or parthenogenetic eggs, the hormones LH, FSH, TSH, and PRL were located mainly in the micromeres, and the staining in the macromeres was weak. On the other hand, in the mictic or sexual eggs the inner shell is stained for the hormones PRL and LH, opposite to the staining of FSH and TSH, located mainly in the embryo. In general, immuno-reactivity was observed in areas important for the reproductive, excretory, digestive and developmental processes. Rev. Biol. Trop. 57 (4: 1049-1058. Epub 2009 December 01.Se logró detectar la presencia de las hormonas: Hormona Luteinizante (LH, Hormona Folículo Estimulante (FSH, Hormona Estimulante de la Tiroides (TSH y Prolactina (PRL en Brachionus calyciflorus siendo el primer reporte de la presencia de dichas hormonas en rotíferos. Estas hormonas fueron

  11. Effects of different batches of /sup 125/iodine on properties of /sup 125/I-hFSH and characteristics of radioligand-receptor assays

    Energy Technology Data Exchange (ETDEWEB)

    Melson, B.E.; Sluss, P.M.; Reichert, L.E. Jr.

    1987-02-01

    Radioiodination of highly purified human follicle-stimulating hormone (hFSH) (4000 IU/mg) was performed every other week for 23 weeks using 2 mCI carrier free Na/sup 125/I (Amersham Corp., 15 mCi/micrograms I2) in the presence of lactoperoxidase. Incorporation of /sup 125/I into hFSH was determined by the method of R. C. Greenwood, W. M. Hunter, and J. S. Grover (1963) Biochem. J. 89, 114). Hormone binding was studied in vitro under steady-state conditions (16 h, 20 degrees C) using different calf testis membrane preparations having similar receptor characteristics. Each /sup 125/I-hFSH preparation was characterized for maximum bindability, specific activity of bindable radioligand as determined by self-displacement analysis, and by determination of Ka and Rt. Incorporation of /sup 125/I into FSH was relatively constant over the large number of experiments (62.4 +/- 6.4 microCi/micrograms; n = 23). By comparison, however, specific radioactivity of the receptor bindable fraction of /sup 125/I-hFSH was related to the lot of /sup 125/I utilized, and was significantly (P less than or equal to 0.01) lower and more variable (28.7 +/- 10.5 microCi/micrograms). Maximum bindability of /sup 125/I-hFSH was not correlated to specific activity (r = 0.06) but was negatively correlated to hFSH /sup 125/I incorporation (r = -0.47; P less than or equal to 0.05). These observations demonstrate the need to assess the quality of each batch of radioligand before undertaking radioligand-receptor assays and suggest that differences in Na/sup 125/I lots affect specific radioactivity of the radioligand and its receptor binding characteristics.

  12. Targeting incentives to reduce habitat fragmentation

    Science.gov (United States)

    David Lewis; Andrew Plantinga; Junjie Wu

    2009-01-01

    This article develops a theoretical model to analyze the spatial targeting of incentives for the restoration of forested landscapes when wildlife habitat can be enhanced by reducing fragmentation. The key theoretical result is that the marginal net benefits of increasing forest can be convex, in which case corner solutions--converting either none or all of the...

  13. Involvement of ERK1/2 signaling pathway in atrazine action on FSH-stimulated LHR and CYP19A1 expression in rat granulosa cells

    Energy Technology Data Exchange (ETDEWEB)

    Fa, Svetlana; Pogrmic-Majkic, Kristina; Samardzija, Dragana; Glisic, Branka; Kaisarevic, Sonja; Kovacevic, Radmila; Andric, Nebojsa, E-mail: nebojsa.andric@dbe.uns.ac.rs

    2013-07-01

    Worldwide used herbicide atrazine is linked to reproductive dysfunction in females. In this study, we investigated the effects and the mechanism of atrazine action in the ovary using a primary culture of immature granulosa cells. In granulosa cells, follicle-stimulating hormone (FSH) activates both cyclic adenosine monophosphate (cAMP) and extracellular-regulated kinase 1/2 (ERK1/2) cascades, with cAMP pathway being more important for luteinizing hormone receptor (LHR) and aromatase (CYP19A1) mRNA expression. We report that 48 h after atrazine exposure the FSH-stimulated LHR and CYP19A1 mRNA expression and estradiol synthesis were decreased, with LHR mRNA being more sensitive to atrazine than CYP19A1 mRNA. Inadequate acquisition of LHR in the FSH-stimulated and atrazine-exposed granulosa cells renders human chorionic gonadotropin (hCG) ineffective to stimulate amphiregulin (Areg), epiregulin (Ereg), and progesterone receptor (Pgr) mRNA expression, suggesting anti-ovulatory effect of atrazine. To dissect the signaling cascade involved in atrazine action in granulosa cells, we used U0126, a pharmacological inhibitor of ERK1/2. U0126 prevents atrazine-induced decrease in LHR and CYP19A1 mRNA levels and estradiol production in the FSH-stimulated granulosa cells. ERK1/2 inactivation restores the ability of hCG to induce expression of the ovulatory genes in atrazine-exposed granulosa cells. Cell-based ELISA assay revealed that atrazine does not change the FSH-stimulated ERK1/2 phosphorylation in granulosa cells. The results from this study reveal that atrazine does not affect but requires ERK1/2 phosphorylation to cause decrease in the FSH-induced LHR and CYP19A1 mRNA levels and estradiol production in immature granulosa cells, thus compromising ovulation and female fertility. - Highlights: • Atrazine inhibits estradiol production in FSH-stimulated granulosa cells. • Atrazine inhibits LHR and Cyp19a1 mRNA expression in FSH-stimulated granulosa cells. • Atrazine

  14. Green Turtle Critical Habitat

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These data represent the critical habitat for green turtle as designated by Federal Register Vol. 63, No. 46701, September 2, 1998, Rules and Regulations.

  15. VT Wildlife Linkage Habitat

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) The Wildlife Linkage Habitat Analysis uses landscape scale data to identify or predict the location of potentially significant wildlife linkage...

  16. Designated Critical Habitat

    Data.gov (United States)

    Kansas Data Access and Support Center — Critical habitats include those areas documented as currently supporting self-sustaining populations of any threatened or endangered species of wildlife as well as...

  17. Habitats, activities, and signs

    DEFF Research Database (Denmark)

    Andersen, Peter Bøgh; Brynskov, Martin

    2004-01-01

    Digital habitats is a framework for designing and modeling environments for activities that involve mobile and embedded computing systems. This paper 1) introduces the basic concepts of the framework, i.e. activity, thematic role, and the three ‘dimensions’ of a habitat: physical, informational, ......, and pragmatic, 2) proposes a notation, and 3) sketches a method and exemplifies areas of application using authentic cases from hospital work, primary school education, the maritime domain, and other areas......Digital habitats is a framework for designing and modeling environments for activities that involve mobile and embedded computing systems. This paper 1) introduces the basic concepts of the framework, i.e. activity, thematic role, and the three ‘dimensions’ of a habitat: physical, informational...

  18. Green Turtle Critical Habitat

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These data represent the critical habitat for green turtle as designated by Federal Register Vol. 63, No. 46701, September 2, 1998, Rules and Regulations.

  19. Right Whale Critical Habitat

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for Right Whale as designated by Federal Register Vol. 59, No. 28805, May 19, 1993, Rules and Regulations.

  20. Habitat Mapping Camera (HABCAM)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset entails imagery collected using the HabCam towed underwater vehicle and annotated data on objects or habitats in the images and notes on image...

  1. Green Turtle Critical Habitat

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for green turtle as designated by Federal Register Vol. 63, No. 46701, September 2, 1998, Rules and Regulations.

  2. Critical Habitat Designations

    Data.gov (United States)

    Department of Homeland Security — The Endangered Species Act (ESA) requires the Federal government to designate 'critical habitat' for any species it lists under the ESA. This dataset combines both...

  3. Johnsons Seagrass Critical Habitat

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for Johnson's Seagrass as designated by Federal Register Vol. 65, No. 66, Wednesday, April 5, 2000, Rules and Regulations.

  4. Smalltooth Sawfish Critical Habitat

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for smalltooth sawfish (Pristis pectinatat) as designated by 74 FR 45353, September 2, 2009, Rules and Regulations.

  5. Infertility and ovarian follicle reserve depletion are associated with dysregulation of the FSH and LH receptor density in human antral follicles.

    Science.gov (United States)

    Regan, Sheena L P; Knight, Phil G; Yovich, John L; Stanger, James D; Leung, Yee; Arfuso, Frank; Dharmarajan, Arun; Almahbobi, Ghanim

    2017-02-07

    The low take-home baby rate in older women in Australia (5.8%) undergoing IVF (5.8%) is linked to the depletion of the ovarian reserve of primordial follicles. Oocyte depletion causes an irreversible change to ovarian function. We found that the young patient FSH receptor and LH receptor expression profile on the granulosa cells collected from different size follicles were similar to the expression profile reported in natural cycles in women and sheep. This was reversed in the older patients with poor ovarian reserve. The strong correlation of BMPR1B and FSH receptor density in the young was not present in the older women; whereas, the LH receptor and BMPR1B correlation was weak in the young but was strongly correlated in the older women. The reduced fertilisation and pregnancy rate was associated with a lower LH receptor density and a lack of essential down-regulation of the FSH and LH receptor. The mechanism regulating FSH and LH receptor expression appears to function independently, in vivo, from the dose of FSH gonadotrophin, rather than in response to it. Restoring an optimum receptor density may improve oocyte quality and the pregnancy rate in older women.

  6. Evaluación de respuesta ovárica y calidad de ovocitos en gatas tratadas con hormona folículo estimulante (FSH utilizando dos esquemas de administración Evaluation of ovarian response and oocyte quality in domestic cats treated with two administration schemes of follicle stimulating hormone (FSH

    Directory of Open Access Journals (Sweden)

    A. E. SANCHEZ

    2003-01-01

    Full Text Available El objetivo de este trabajo fue estudiar el efecto del tratamiento gonadotrófico con FSH sobre las características foliculares y ovocitarias de gatas adultas, y comparar dos esquemas de administración de FSH en términos del número de ovocitos potencialmente aptos para MIV. Usando veinte gatas adultas, aleatoriamente, se formaron tres grupos de tratamiento. Grupo SC (n=7 5 mg/ día de FSH por 4 días, vía subcutánea; Grupo IM (n=6 2 mg/día de FSH por 5 días, vía intramuscular y un Grupo Control (n=7. Las gatas fueron ovariectomizadas y se realizó recuento, medición de folículos y recuperación de ovocitos. Los folículos fueron agrupados en dos categorías: o = 2 mm (considerados como preovulatorios. El tratamiento gonadotrófico aumentó (P o = 2 mm, comparado con el grupo control, independiente del esquema de administración de FSH. El tratamiento gonadotrófico, también aumentó (P o = 2 mm versus folículosThe purpose of this work was to study the effects of FSH administration upon follicular and oocyte characteristics of female cats. Two FSH administration schemes were compared and their effects on the number of follicles and good grade oocytes for IVM obtained by follicular punction was determined. Twenty adults female cats were randomly distributed on 3 experimental groups: Group SC (n=7 recieved 5 mg/day of FSH for 4 days, subcutaneoulsy; Group IM (n=6 recieved 2 mg/day of FSH for 5 days, intramuscular and Control Group (n=7 which did not recieve any treatment. The ovaries were surgically removed from all female cats and follicles were counted and measured and the oocytes were recovered. Follicles were clasified according to their diameter on or = 2 mm (preovulatory. Gonadotrophine treatment increased the total number of follicles and follicles with > or = 2 mm diameter when compared to control group (P or = 2 mm than for follicles with < 2 mm diameter. It can be concluded that intramuscular or subcutaneus administration of

  7. Rebuilding Habitat and Shoreline Resilience through Improved Flood Control Project

    Science.gov (United States)

    Information about the SFBWQP Rebuilding Habitat and Shoreline Resilience through Improved Flood Control Project, part of an EPA competitive grant program to improve SF Bay water quality focused on restoring impaired waters and enhancing aquatic resources.

  8. Determination of biological activity of gonadotropins hCG and FSH by Förster resonance energy transfer based biosensors.

    Science.gov (United States)

    Mazina, Olga; Allikalt, Anni; Tapanainen, Juha S; Salumets, Andres; Rinken, Ago

    2017-02-09

    Determination of biological activity of gonadotropin hormones is essential in reproductive medicine and pharmaceutical manufacturing of the hormonal preparations. The aim of the study was to adopt a G-protein coupled receptor (GPCR)-mediated signal transduction pathway based assay for quantification of biological activity of gonadotropins. We focussed on studying human chorionic gonadotropin (hCG) and follicle-stimulating hormone (FSH), as these hormones are widely used in clinical practice. Receptor-specific changes in cellular cyclic adenosine monophosphate (cAMP, second messenger in GPCR signalling) were monitored by a Förster resonance energy transfer (FRET) biosensor protein (T)Epac(VV) in living cells upon activation of the relevant gonadotropin receptor. The BacMam gene delivery system was used for biosensor protein expression in target cells. In the developed assay only biologically active hormones initiated GPCR-mediated cellular signalling. High assay sensitivities were achieved for detection of hCG (limit of detection, LOD: 5 pM) and FSH (LOD: 100 pM). Even the small-scale conformational changes caused by thermal inactivation and reducing the biological activity of the hormones were registered. In conclusion, the proposed assay is suitable for quantification of biological activity of gonadotropins and is a good alternative to antibody- and animal-testing-based assays used in pharmaceutical industry and clinical research.

  9. Determination of biological activity of gonadotropins hCG and FSH by Förster resonance energy transfer based biosensors

    Science.gov (United States)

    Mazina, Olga; Allikalt, Anni; Tapanainen, Juha S.; Salumets, Andres; Rinken, Ago

    2017-01-01

    Determination of biological activity of gonadotropin hormones is essential in reproductive medicine and pharmaceutical manufacturing of the hormonal preparations. The aim of the study was to adopt a G-protein coupled receptor (GPCR)-mediated signal transduction pathway based assay for quantification of biological activity of gonadotropins. We focussed on studying human chorionic gonadotropin (hCG) and follicle-stimulating hormone (FSH), as these hormones are widely used in clinical practice. Receptor-specific changes in cellular cyclic adenosine monophosphate (cAMP, second messenger in GPCR signalling) were monitored by a Förster resonance energy transfer (FRET) biosensor protein TEpacVV in living cells upon activation of the relevant gonadotropin receptor. The BacMam gene delivery system was used for biosensor protein expression in target cells. In the developed assay only biologically active hormones initiated GPCR-mediated cellular signalling. High assay sensitivities were achieved for detection of hCG (limit of detection, LOD: 5 pM) and FSH (LOD: 100 pM). Even the small-scale conformational changes caused by thermal inactivation and reducing the biological activity of the hormones were registered. In conclusion, the proposed assay is suitable for quantification of biological activity of gonadotropins and is a good alternative to antibody- and animal-testing-based assays used in pharmaceutical industry and clinical research. PMID:28181555

  10. Habitat Evaluation Procedures (HEP) Report; Gamblin Lake, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    On August 12, 2003, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Gamblin Lake property, an acquisition completed by the Kalispel Tribe of Indians in December 2002. Evaluation species and appropriate models include bald eagle, black-capped chickadee, mallard, muskrat, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Gamblin Lake Project provides a total of 273.28 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 127.92 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Forested wetland habitat provides 21.06 HUs for bald eagle, black-caped chickadee, mallard, and white-tailed deer. Wet meadow provides 78.05 HUs for Canada goose and mallard. Emergent wetland habitat provides 46.25 HUs for mallard, muskrat, and Canada goose. The objective of using HEP at the Gamblin Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  11. Habitat Evaluation Procedures (HEP) Report; Beaver Lake, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    On August 14, 2003, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Beaver Lake property, an acquisition completed by the Kalispel Tribe of Indians in November 2002. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Beaver Lake Project provides a total of 232.26 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 136.58 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Forested wetland habitat provides 20.02 HUs for bald eagle, black-caped chickadee, mallard, and white-tailed deer. Scrub-shrub wetland habitat provides 7.67 HUs for mallard, yellow warbler, and white-tailed deer. Grassland meadow provides 22.69 HUs for Canada goose and mallard. Emergent wetlands provide 35.04 HUs for Canada goose, mallard, and muskrat. Open water provided 10.26 HUs for Canada goose, mallard, and muskrat. The objective of using HEP at the Beaver Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  12. Habitat Choice and Speciation

    Directory of Open Access Journals (Sweden)

    Sophie E. Webster

    2012-01-01

    Full Text Available The role of habitat choice in reproductive isolation and ecological speciation has often been overlooked, despite acknowledgement of its ability to facilitate local adaptation. It can form part of the speciation process through various evolutionary mechanisms, yet where habitat choice has been included in models of ecological speciation little thought has been given to these underlying mechanisms. Here, we propose and describe three independent criteria underlying ten different evolutionary scenarios in which habitat choice may promote or maintain local adaptation. The scenarios are the result of all possible combinations of the independent criteria, providing a conceptual framework in which to discuss examples which illustrate each scenario. These examples show that the different roles of habitat choice in ecological speciation have rarely been effectively distinguished. Making such distinctions is an important challenge for the future, allowing better experimental design, stronger inferences and more meaningful comparisons among systems. We show some of the practical difficulties involved by reviewing the current evidence for the role of habitat choice in local adaptation and reproductive isolation in the intertidal gastropod Littorina saxatilis, a model system for the study of ecological speciation, assessing whether any of the proposed scenarios can be reliably distinguished, given current research.

  13. Physico-chemical characterization of human recombinant follicle-stimulating hormone (hFSH) and its subunits by reversed-phase high-performance liquid chromatography ( RP-HPLC): comparison with pituitary hFSH reference preparation from 'National Hormone and Pituitary Program' from USA; Caracterizacao fisico-quimica da foliculotropina humana(hFSH) recombinabte e de suas subunidades, por cromatografia liquida de alta eficiencia (HPLC) em fase reversa: comparacao com a preparacao de referencia de hFSH de origem hipofisaria do ''National Hormone and Pituitary Program'' dos EUA

    Energy Technology Data Exchange (ETDEWEB)

    Loureiro, Renan Fernandes

    2006-07-01

    A reversed-phase high-performance liquid chromatography (RP-HPLC) method for the qualitative and quantitative analysis of intact human folliclestimulating hormone (hFSH) was established and validated for accuracy, precision and sensitivity. Human FSH is a dimeric glycoprotein hormone widely used as a diagnostic analyte and as therapeutic product in reproductive medicine. The technique developed preserves the protein integrity, allowing the analysis of the intact heterodimeric form rather than just of its subunits, as it is the case for the majority of the conditions currently employed. This methodology has also been employed for comparing the relative hydrophobicity of pituitary, urinary and two Chinese hamster ovary (CHO)-derived hFSH preparations, as well as of two other related glycoprotein hormones of the anterior pituitary: human thyroid-stimulating hormone (hTSH) and human luteinizing hormone (hLH). The least hydrophobic of the three glycohormones analyzed was hFSH, followed by hTSH and hLH. A significant difference (p<0.005) was observed in t{sub R} between the pituitary and recombinant hFSH preparations, reflecting structural differences in their carbohydrate moieties. Two main isoforms were detected in urinary hFSH, including a form which was significantly different (p<0.005) for the pituitary and recombinant preparations. The linearity of the dose-response curve (r = 0.9965, n = 15) for this RP-HPLC methodology, as well as an inter-assay precision with relative standard deviation less than 4% for the quantification of different hFSH preparations and a sensitivity of the order of 40 ng, were demonstrated. The chromatographic behavior and relative hydrophobicity of the individual subunits of the pituitary and recombinant preparations were also analyzed. Furthermore, the accurate molecular mass of the individual hFSH subunits and of the heterodimer were simultaneously determined by matrix-assisted laser desorption ionization time-of-flight mass spectral

  14. Cost-effectiveness analysis on the use of rFSH + rLH for the treatment of anovulation in hypogonadotropic hypogonadal women

    Directory of Open Access Journals (Sweden)

    Papaleo E

    2014-06-01

    Full Text Available Enrico Papaleo,1 Carlo Alviggi,2 Giorgio Lorenzo Colombo,3,4 Claudio Pisanelli,5,6 Claudio Ripellino,7 Salvatore Longobardi,8 Pier Luigi Canonico91Centro Scienze della Natalità, Gynecological-Obstetrics Department, San Raffaele Hospital, Vita-Salute San Raffaele, Milan, Italy; 2Department of Neuroscience, Reproductive Sciences and Odontostomatology, University “Federico II” of Naples, Naples, Italy; 3Department of Drug Sciences, University of Pavia, Pavia, Italy; 4SAVE Studi Analisi Valutazioni Economiche, Milan, Italy; 5ACO San Filippo Neri, Rome, Italy; 6Società Italiana Di Farmacia Ospedaliera, Milan, Italy; 7CSD Medical Research Srl, Milan, Italy; 8Medical Department, Merck Serono SpA, Rome, Italy; 9Department of Pharmaceutical Sciences, University of Piemonte Orientale, Novara, Italy Background: Hypogonadotropic hypogonadal women are characterized by ovarian functionality deficiency, caused by low concentrations of follicle-stimulating hormone (FSH and luteinizing hormone (LH. To recover reproduction functionality, recommended therapies for ovarian induction involve injections of FSH and LH medications. Objective: Since important differences exist between recombinant and urinary gonadotropin therapies in terms of efficacy and cost, the objective of this study was to develop a cost-effectiveness model to compare recombinant FSH (rFSH + recombinant LH (rLH and highly purified human menopausal gonadotropin (HP-HMG. Methods: A Markov model was developed, considering three cycles of therapy; probability of pregnancy and miscarriage were considered, and the efficacy was evaluated in terms of pregnancy occurrence. The perspective of the model was that of the Italian Health Service, so only direct cost (drugs, specialist visits, patient examinations, and hospitalizations were included. Results: rFSH + rLH is associated with a higher total cost (€3,453.50 and higher efficacy (0.87 compared with HP-HMG (€2,719.70 and 0.50. rFSH + r

  15. Regulative Actions of the Chinese Drugs for Tonifying the Kidney on Gene Expression of the Hypothalamic GnRH, Pituitary FSH, LH and Osteoblastic BGP

    Institute of Scientific and Technical Information of China (English)

    蔡德培; 张炜

    2005-01-01

    It is found that the drugs for nourishing yin to reduce pathogenic fire can significantly down-regulate, and the drags for tonifying the kidney to replenish essence can up-regulate mRNA expression of the hypothalamic GnRH, pituitary FSH, LH and osteoblastic BGP, indicating that the Chinese drugs for tonifying the kidney can regulate gene expression of the hypothalamic GnRH, pituitary FSH, LH, and osteoblastic BGP, which is possibly one of the main mechanisms of the Chinese drug for tonifying the kidney, regulating ephebic development process and improving skeletal development in sexual precocity children.

  16. Production of recombinant orange-spotted grouper (Epinephelus coioides) follicle-stimulating hormone (FSH) in single-chain form and dimer form by Pichia pastoris and their biological activities.

    Science.gov (United States)

    Chen, Jun; Zhang, Yanhong; Tang, Zhiguo; Mao, Jiewei; Kuang, Zhonglei; Qin, Chaobin; Li, Wensheng

    2012-09-01

    FSH is a key regulator of steroidogenesis and gonadal growth in teleosts. However, function of FSH is elusive in grouper due to the lack of purified and native FSH. In the present study, we reported production of bioactive orange-spotted grouper (Epinephelus coioides) FSH in dimer form and single-chain form by Pichia pastoris. Dimer form of recombinant grouper FSH (rgFSHba) was accomplished by co-expressing mature FSHb-subunit and a-subunit genes. Fusion of mature FSHb-subunit and a-subunit genes together linking with a polypeptide (4×(Gly-Ser)-Gly-Thr) gene generated single-chain form of recombinant grouper FSH (rgFSHb-a). Recombinant grouper common α-subunit (rgCga) and FSHb-subunit (rgFSHb) were also separately produced. Recombinant proteins were verified by Western blot and mass spectrometry assays, and characterized by deglycosylation analysis. Deglycosylation assay suggested that glycosylation of recombinant FSH mainly occurred on common a-subunit. Bioactivities of recombinant proteins were initially evaluated by activating grouper FSH receptor, and further demonstrated by incubating ovarian fragments of adult grouper and intraperitoneal injection in juvenile female grouper. Two forms of recombinant FSH presented similar biological activities of activating FSH receptor and stimulating in vitro testosterone (T) and estradiol-17β (E2) secretion, though the dimer form functioned slightly weaker than the single-chain form. However, injections of rgFSHb-a or rgFSHba could significantly increase serum T and E2 levels, induce early ovarian development, reduce hypothalamic gnrh1 mRNA level, and increase hypothalamic cyp19a1b mRNA level. Data in this study suggested that recombinant gonadotropin could be produced in dimer form or single-chain form by P. pastoris, and FSH could regulate steroidogenesis and early ovarian development in juvenile grouper.

  17. IDENTIFICATION OF POLYMORPHISM OF FSH BETA-SUBUNIT GENE AS SPERM QUALITY MARKER IN BALI CATTLE USING PCR-RFLP

    Directory of Open Access Journals (Sweden)

    A.B.L. Ishak

    2014-10-01

    Full Text Available The aim of study was to identify the association of FSH beta-subunit gene polymorphisms withsperm quality traits. A total of 470 samples of normal mature bull from several breeds were used forpopulation study and 127 bulls from National and Regional AI centre of Indonesia for association study.To amplify, a PCR-RFLP method was used and digested with Pst1 restriction enzyme. The allelefrequency of the A and B in Bali cattle were (0.000 and (1.000, respectively. The absence of otherallele A suggested that the Bali cattle was monomorphic, while Brahman, FH, Simmental and Limousinewere polymorphic. The highest observed heterozygosity were found in Limousine (0.318 and thehighest expected heterozygosity were in Simmental (0.420. The higher incident of percentage of spermabnormalities were found in Simmental, Limousin, Brahman compared to Bali and FH. Among all typesof sperm abnormalities, the abaxial and microcephalus were found in highest number.

  18. Corifollitropin alfa followed by rFSH in a GnRH antagonist protocol for poor ovarian responder patients

    DEFF Research Database (Denmark)

    Polyzos, Nikolaos P; Devos, Michel; Humaidan, Peter;

    2013-01-01

    OBJECTIVE: To identify whether women with poor ovarian response may benefit from treatment with corifollitropin alfa in a GnRH antagonist protocol. DESIGN: Retrospective pilot study. SETTING: University-based tertiary care center. PATIENT(S): Poor ovarian responders fulfilling the Bologna criteria...... developed by European Society for Human Reproduction and Embryology Consensus Group. INTERVENTION(S): Corifollitropin alfa (150 μg) followed by 300 IU rFSH in a GnRH antagonist protocol. MAIN OUTCOME MEASURE(S): Endocrinologic profile and ongoing pregnancy rates. RESULT(S): Among 43 women treated...... compared with a cohort of patients treated during 2011 with the standard protocol for poor responders in our center (short agonist-hMG) (7% vs. 6.3%). CONCLUSION(S): Treatment of poor ovarian responders, as described by the Bologna criteria, with corifollitropin alfa in a GnRH antagonist protocol results...

  19. Han Chinese polycystic ovary syndrome risk variants in women of European ancestry: relationship to FSH levels and glucose tolerance.

    Science.gov (United States)

    Saxena, R; Georgopoulos, N A; Braaten, T J; Bjonnes, A C; Koika, V; Panidis, D; Welt, C K

    2015-06-01

    Are PCOS risk variants identified in women of Han Chinese ethnicity also associated with risk of PCOS or the phenotypic features of PCOS in European women? One variant, rs2268361-T, in the intron of FSHR was associated with PCOS and lower FSH levels, while another variant rs705702-G near the RAB5B and SUOX genes was associated with insulin and glucose levels after oral glucose testing in women with PCOS of European ethnicity. Three of the eleven variants associated with PCOS in the Han Chinese genome-wide association studies were also associated with PCOS in at least one European population when corrected for multiple testing (DENND1A, THADA and YAP1). However, additional replication is needed to establish the importance of these variants in European women and to determine the relationship to PCOS phenotypic traits. The study was a case-control examination in a discovery cohort of women with PCOS (n = 485) and controls (n = 407) from Boston (Boston 1). Replication was performed in women from Greece (cases n = 884 and controls n = 311) and an additional cohort from Boston (Boston electronic medical record (EMR); n = 350 cases and n = 1258 controls). Women had PCOS defined by the National Institutes of Health criteria in Boston 1 and Greece (n = 783), with additional subjects fulfilling the Rotterdam criteria (hyperandrogenism, polycystic ovary morphology and regular menses) in Greece (n = 101). Controls in Boston and Greece had regular menstrual cycles and no hyperandrogenism. The second cohort from Boston was defined using the EMR and natural language processing. Allele frequencies for variants associated with PCOS in Han Chinese women were examined in PCOS cases and controls, along with the relationship to quantitative traits. A variant rs2268361-T in an intron of FSHR was associated with PCOS (0.84 [0.76-0.93], OR [95% CI]; P = 0.002). The rs2268361-T was associated with lower FSH levels (-0.15 ± 0.05; P = 0.0029). A variant rs705702-G near RAB5B and SUOX was

  20. A double-blind, non-inferiority RCT comparing corifollitropin alfa and recombinant FSH during the first seven days of ovarian stimulation using a GnRH antagonist protocol

    NARCIS (Netherlands)

    Devroey, P.; Boostanfar, R.; Koper, N. P.; Mannaerts, B. M. J. L.; IJzerman-Boon, P. C.; Fauser, B. C. J. M.

    2009-01-01

    Corifollitropin alfa, a fusion protein lacking LH activity, has a longer elimination half-life and extended time to peak levels than recombinant FSH (rFSH). A single injection of corifollitropin alfa may replace seven daily gonadotrophin injections during the first week of ovarian stimulation. In th

  1. Superovulação em caprinos da raça Moxotó com FSH-p

    Directory of Open Access Journals (Sweden)

    Alice Andrioli

    2000-01-01

    Full Text Available O objetivo deste trabalho foi avaliar as taxas de manifestação de sintomas de estro, de resposta superovulatória e de recuperação de embriões em 20 cabras da raça Moxotó em três tratamentos consecutivos, em intervalos de 56 dias. O ciclo estral das fêmeas foi sincronizado com esponjas vaginais contendo 60 mg de acetato de medroxiprogesterona (MGA durante 11 dias e com 100 mig de cloprostenol (PGF2alfa administrado no nono dia. Neste dia, iniciou-se o tratamento superovulatório com 250 UI de FSH-p por cabra, fracionadas em oito doses decrescentes, com intervalo de 12 horas. A repetição do tratamento superovulatório com FSH-p diminuiu a taxa de manifestação de sintomas de estro, porém não afetou a taxa de ovulação e reduziu a taxa de regressão prematura de corpos lúteos. A taxa de recuperação de embriões foi influenciada pela ocorrência de regressão prematura de corpos lúteos e pela ação das repetidas colheitas de embriões sobre o genital. Não foi possível avaliar o efeito das repetidas superovulações sobre a taxa de recuperação de embriões.

  2. Downregulation of LH and FSH receptors after hCG and eCG treatments in the porcine oviduct.

    Science.gov (United States)

    Małysz-Cymborska, I; Andronowska, A

    2016-10-01

    The influence of induction of ovulation and superovulation with eCG and hCG on LH and FSH receptor levels in porcine oviducts on day 3 postcoitum was studied. In experiment I, gilts were assigned into cyclic (control; n = 5) and inseminated (n = 5) groups. In experiment II, there were 3 groups of animals: inseminated (n = 5), induced ovulation/inseminated (750 IU eCG, 500 IU hCG; n = 5) and superovulated/inseminated (1500 IU eCG, 1000 IU hCG; n = 5) gilts. Oviduct tissues were collected 3 d after insemination or PBS infusion. The messenger RNA (mRNA) expression of FSH receptor (FSHR) and luteinizing hormone/chorionic gonadotropin receptor (LH/CGR) was measured by real-time reverse transcription PCR and protein levels using Western blots. Localization of LH/CGR and FSHR-positive cells was studied by immunohistochemical staining. Insemination by itself did not influence mRNA and protein levels of LH/CGR. However, FSHR mRNA expression in the isthmus and ampulla of the oviduct was affected by insemination (P hCG and eCG did not affect LH/CGR and FSHR mRNA expression, either in the isthmus or in the ampulla. Nevertheless, superovulation decreased LH/CGR protein level in the oviductal ampulla (P hCG, especially in high doses, can change LH/CGR and FSHR levels in porcine oviducts. This may in turn alter many signaling pathways, eg, PGs or vascular endothelial growth factor synthesis, and consequently disturb the oviductal environment, with possible detrimental effects on fertilization and/or embryonic development. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Intrafollicular endocrine milieu after addition of hCG to recombinant FSH during controlled ovarian stimulation for in vitro fertilization.

    Science.gov (United States)

    Thuesen, L L; Andersen, A Nyboe; Loft, A; Smitz, J

    2014-02-01

    The role of human chorionic gonadotropin (hCG) supplementation on the intrafollicular steroid milieu has been studied. The objective of the study was to assess the impact on steroid levels in follicular fluids (FFs) after different doses of hCG supplementation to recombinant FSH for controlled ovarian stimulation. This was a prospective randomized dose-response study conducted at Copenhagen University Hospital, Rigshospitalet, Denmark. From 62 in vitro fertilization patients, 334 FFs were selected for analyses. Patients were treated using a GnRH agonist protocol with recombinant FSH 150 IU/d and randomized from stimulation day 1 to supplementation with hCG: D0, 0 IU/d; D50, 50 IU/d; D100, 100 IU/d; and D150, 150 IU/d. Intrafollicular hormone concentrations in relation to treatment groups, follicular sizes, and embryo quality were measured. In large follicles, hCG supplementation induced a nearly 3-fold increase of estradiol (nanomoles per liter) [D0: 1496; D50: 3138; D100: 4338; D150: 4009 (P D100: 72; D150: 56 (P D100 and the highest in D0. Large follicles giving rise to good-quality embryos had significantly higher estradiol and progesterone levels and estradiol to T, estradiol to androstenedione, and progesterone to estradiol ratios, compared with small follicles, leading to poor-quality embryos. Increasing doses of hCG supplementation markedly stimulated the intrafollicular concentration of both estradiol and androgens, with a shift toward a more androgenic milieu. In large follicles with oocytes giving rise to good-quality embryos, the FFs were significantly more estrogenic than in small follicles with oocytes developing into poor quality embryos.

  4. Análogo de GnRH disminuye la secreción de hormona folículo estimulante (FSH en ovejas prepúberes A gonadotropin releasing hormone analogue decreases follicle stimulating hormone (FSH secretion in prepubertal female sheep

    Directory of Open Access Journals (Sweden)

    S E Recabarren

    2006-01-01

    Full Text Available La secreción de LH y FSH depende de la liberación pulsátil de GnRH desde el hipotálamo; sin embargo, el grado de dependencia así como la relación entre los pulsos de GnRH y la secreción de FSH es menos clara. Experimentos en monos han mostrado que altas frecuencias de liberación de pulsos de GnRH estimulan preferentemente la secreción de LH, mientras que bajas frecuencias de pulsos de GnRH favorecen la secreción de FSH. Estudios sobre el control de la secreción de FSH en ovejas prepúberes son escasos. Con el objetivo de reconocer la dependencia de la secreción de FSH por parte de la GnRH, se administró un análogo de GnRH en microcápsulas de liberación lenta (Trp6-GnRH, Decapeptyl® y cuyo efecto bloqueador sobre la secreción de LH ha sido demostrado. El análogo se administró intramuscularmente cada cuatro semanas a partir de las 20 semanas de edad a cinco borregas Suffolk por tres veces. Otro grupo de ovejas prepúberes de las mismas edades recibió el vehículo. Estudios de pulsatilidad de FSH se efectuaron a las, 20, 26 y 30 semanas de edad. Se utilizó el programa Cluster para reconocer las características de la secreción pulsátil de FSH. En las borregas del grupo control no hubo cambios en las características de la secreción pulsátil de FSH entre las 20 y 30 semanas de edad. En el grupo tratado con el análogo de GnRH, las concentraciones plasmáticas de FSH disminuyeron desde 3,4±0,3 ng/mL/5h a las 20 semanas de edad hasta los 0,36±0,1 ng/mL/5h a las 30 semanas de edad (pLH and FSH secretions depend on the pulsatile secretion of GnRH from the hypothalamus. However, the grade of dependency as well as the relationship between pulses of GnRH and pulses of FSH secretion is less clear. Experiments in monkeys have shown that high GnRH pulse frequency favors LH secretion while low frequency of GnRH pulses preferentially stimulates the FSH secretion. The objective of the present work was to recognize the dependence of Gn

  5. Wildlife habitat fragmentation.

    Science.gov (United States)

    John. Lehmkuhl

    2005-01-01

    A primary issue in forest wildlife management is habitat fragmentation and its effects on viability, which is the "bottom line" for plant and animal species of conservation concern. Population viability is the likelihood that a population will be able to maintain itself (remain viable) over a long period of time-usually 100 years or more. Though it is true...

  6. Earth is a Marine Habitat. Habitat Conservation Program.

    Science.gov (United States)

    National Oceanic and Atmospheric Administration (DOC), Rockville, MD.

    This brochure is intended to educate the public about the need to conserve and preserve the earth's environment (man's habitat). It contains an introduction to the ocean world and threats to coastal habitat. Photos and narrative revolve around the theme "Earth is a Marine Habitat." Sections include: "The Web of Life,""Oceans and the United…

  7. A randomized assessor-blind trial comparing highly purified hMG and recombinant FSH in a GnRH antagonist cycle with compulsory single-blastocyst transfer

    DEFF Research Database (Denmark)

    Devroey, Paul; Pellicer, Antonio; Nyboe Andersen, Anders;

    2012-01-01

    OBJECTIVE: To compare the efficacy and safety of highly purified menotropin (hphMG) and recombinant FSH (rFSH) for controlled ovarian stimulation in a GnRH antagonist cycle with compulsory single-blastocyst transfer. DESIGN: Randomized, open-label, assessor-blind, parallel groups, multicenter......, noninferiority trial. SETTING: Twenty-five infertility centers in seven countries. PATIENT(S): Seven hundred forty-nine women. INTERVENTION(S): Controlled ovarian stimulation with hphMG or rFSH in a GnRH antagonist cycle with compulsory single-blastocyst transfer on day 5 in one fresh or subsequent frozen......% and 38% for women treated with hphMG and rFSH, respectively (both PP and ITT). Significant differences in pharmacodynamic end points were found between the two gonadotropin preparations. CONCLUSION(S): Highly purified hMG is at least as effective as rFSH in GnRH antagonist cycles with compulsory single...

  8. Archive of side scan sonar and bathymetry data collected during USGS cruise 06FSH01 offshore of Siesta Key, Florida, May 2006.

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set contains swath bathymetric data collected during USGS cruise 06FSH01 aboard the R/V G.K. Gilbert. A side scan sonar, bathymetric, and high-resolution...

  9. Archive of side scan sonar and bathymetry data collected during USGS cruise 06FSH01 offshore of Siesta Key, Florida, May 2006.

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set contains swath bathymetric data collected during USGS cruise 06FSH01 aboard the R/V G.K. Gilbert. A side scan sonar, bathymetric, and high-resolution...

  10. A randomized controlled trial investigating the use of a predictive nomogram for the selection of the FSH starting dose in IVF/ICSI cycles.

    Science.gov (United States)

    Allegra, Adolfo; Marino, Angelo; Volpes, Aldo; Coffaro, Francesco; Scaglione, Piero; Gullo, Salvatore; La Marca, Antonio

    2017-01-23

    The number of oocytes retrieved is a relevant intermediate outcome in women undergoing IVF/intracytoplasmic sperm injection (ICSI). This trial compared the efficiency of the selection of the FSH starting dose according to a nomogram based on multiple biomarkers (age, day 3 FSH, anti-Müllerian hormone) versus an age-based strategy. The primary outcome measure was the proportion of women with an optimal number of retrieved oocytes defined as 8-14. At their first IVF/ICSI cycle, 191 patients underwent a long gonadotrophin-releasing hormone agonist protocol and were randomized to receive a starting dose of recombinant (human) FSH, based on their age (150 IU if ≤35 years, 225 IU if >35 years) or based on the nomogram. Optimal response was observed in 58/92 patients (63%) in the nomogram group and in 42/99 (42%) in the control group (+21%, 95% CI = 0.07 to 0.35, P = 0.0037). No significant differences were found in the clinical pregnancy rate or the number of embryos cryopreserved per patient. The study showed that the FSH starting dose selected according to ovarian reserve is associated with an increase in the proportion of patients with an optimal response: large trials are recommended to investigate any possible effect on the live-birth rate.

  11. Marcación con yodo125 y radioinmunoanálisis de hormonas folículo estimulantes (FSH y Luteinizante (LH humanas

    Directory of Open Access Journals (Sweden)

    Elsa Múnevar

    2009-06-01

    Full Text Available En el presente trabajo se describe la optimización del Radioinmunoanálisis de las hormonas Folículo Estimulantes (FSH y Luteinizante (LH humanas, estableciendo inicialmente las condiciones de marcación con 125 I por el método de la Cloramina T.

  12. Prediction of Age at Menopause in Women of Suburban Areas in Chennai Using A Model of Fsh Over Age - A Pilot Study

    Directory of Open Access Journals (Sweden)

    SM Priyadharshini

    2014-02-01

    Full Text Available Objectives: The human ovary is characterized by early senescence and the end stage of ovarian activity is termed menopause. The age at which menopause occur is between 45 and 55 years world wide. The objective of this pilot study is to determine the age at menopause by using a model of FSH over age in women of sub urban region around Chennai, India. Materials and Methods: The subjects include 500 patients of age between 30 and 36 yrs with BMI ranging from 24-28.After recording their general profile and history, blood samples were obtained by venipuncture and hormone FSH was estimated on the day 3 of the menstrual cycle. Based on functional dependence of FSH in the form of exponential relation with age, a model was proposed. Using least square approximation the beta values were calculated. Results: With the help of beta values and using the cut off value of 40 IU/ml for FSH, this predicted model determined the age of menopause as 44.6yrs in women of sub urban region around Chennai. Conclusion: The age of menopause is different in various region worlds wide. According to this pilot study the suburban women of Chennai, attain menopause at an age of 44.6 years. Further exploration should be done to alleviate the role of diet, life style and ethnic variation on menopausal age and the impact of chronic disease like osteoporosis during the period of menopause.

  13. Carpinteria Salt Marsh Habitat Polygons

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — We identified five common habitat types in Carpinteria Salt Marsh: channels, pans (flats), marsh, salt flat and upland. We then drew polygons around each habitat...

  14. NORTHWOODS Wildlife Habitat Data Base

    Science.gov (United States)

    Mark D. Nelson; Janine M. Benyus; Richard R. Buech

    1992-01-01

    Wildlife habitat data from seven Great Lakes National Forests were combined into a wildlife-habitat matrix named NORTHWOODS. Several electronic file formats of NORTHWOODS data base and documentation are available on floppy disks for microcomputers.

  15. NEPR Benthic Habitat Map 2015

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This benthic habitat map was created from a semi-automated habitat mapping process, using a combination of bathymetry, satellite imagery, aerial imagery and...

  16. Study of association between ovarian volume with the number of antral follicles and third day of menstruation FSH in infertile patients referred to Omid Persian gulf infertility Clinic

    Directory of Open Access Journals (Sweden)

    Elham Rahmani

    2016-09-01

    Full Text Available Background: Ovarian reserve tests have appeared as important, useful and new tools in evaluation of infertile women and by doing these tests, we can do for infertile couples advanced and necessary measures quickly. The goal of this study was to investigate the association between ovarian volume, Antral follicle counts and serum level of FSH at the third day of menstruation. Material and Methods: This study was conducted on 78 women between 18-49 years old who referred to Omid Persian gulf infertility Clinic with complaining of infertility in 2014. In patients who had inclusion criteria, measurement of ovarian volume were done with calculation of three diameters and the number of antral follicles by using vaginal ultrasound in third day of menstruation. Also, in this patients were measured the levels of FSH&LH in third day of menstruation. Pearson correlation coefficient were used to examine the relationship between ovarian volume and the number of Antral follicles and serum levels. Results: ovarian volume and Antral follicle counts (p=0.0001 decreased and serum level of FSH (p=0.0001 and LH (p=0,022 increased significantly with increasing of age. There was a strong positive correlation between ovarian volume and Antral follicle counts (r=0.96, p=0.0001 and there was a strong reversed correlation between ovarian volume and Antral follicle counts with FSH (r=0.50, p=0.0001. Conclusion: By increasing age, ovarian volume and Antral follicle counts decreased and FSH&LH levels increased. In fact, in the initial study of infertile patients, even by diagnosis of reduction in ovarian volume and Antral follicle count should be performed advanced therapies and there is no need to wait for expensive tests result.

  17. PIVET rFSH dosing algorithms for individualized controlled ovarian stimulation enables optimized pregnancy productivity rates and avoidance of ovarian hyperstimulation syndrome.

    Science.gov (United States)

    Yovich, John L; Alsbjerg, Birgit; Conceicao, Jason L; Hinchliffe, Peter M; Keane, Kevin N

    2016-01-01

    The first PIVET algorithm for individualized recombinant follicle stimulating hormone (rFSH) dosing in in vitro fertilization, reported in 2012, was based on age and antral follicle count grading with adjustments for anti-Müllerian hormone level, body mass index, day-2 FSH, and smoking history. In 2007, it was enabled by the introduction of a metered rFSH pen allowing small dosage increments of ~8.3 IU per click. In 2011, a second rFSH pen was introduced allowing more precise dosages of 12.5 IU per click, and both pens with their individual algorithms have been applied continuously at our clinic. The objective of this observational study was to validate the PIVET algorithms pertaining to the two rFSH pens with the aim of collecting ≤15 oocytes and minimizing the risk of ovarian hyperstimulation syndrome. The data set included 2,822 in vitro fertilization stimulations over a 6-year period until April 2014 applying either of the two individualized dosing algorithms and corresponding pens. The main outcome measures were mean oocytes retrieved and resultant embryos designated for transfer or cryopreservation permitted calculation of oocyte and embryo utilization rates. Ensuing pregnancies were tracked until live births, and live birth productivity rates embracing fresh and frozen transfers were calculated. Overall, the results showed that mean oocyte numbers were 10.0 for all women productivity rates exceeded 50% for women 15 oocytes, significantly lower than recently published data applying conventional dosages (38.2%; Pproducts, with very similar results, and they can be considered validated on the basis of effectiveness and safety, clearly avoiding ovarian hyperstimulation syndrome.

  18. The long-term effects of FSH and triiodothyronine administration during the pubertal period on Connexin 43 expression and spermatogenesis efficiency in adult rats.

    Science.gov (United States)

    Marchlewska, Katarzyna; Slowikowska-Hilczer, Jolanta; Walczak-Jedrzejowska, Renata; Oszukowska, Elzbieta; Filipiak, Eliza; Kula, Krzysztof

    2015-04-01

    Follicle-stimulating hormone (FSH) and triiodothyronine (T3) are known regulatory factors of spermatogenesis initiation. Hyperstimulation of both hormones evokes regressional changes in connexin 43 expression and the seminiferous epithelium in young rats during testicular maturation. However, separate treatments with T3 reduce Sertoli cell number, which seems to be closely connected with the maturation of connexin 43 gap junctions. FSH elevates Sertoli cell number and function, but this effect may take place regardless of the presence of connexin 43-dependent intercellular communication. The aim of the study was to evaluate the later effects of such treatments. Newborn, male Wistar rats were divided randomly into experimental groups receiving daily subcutaneous injections of either 7.5 IU/animal FSH, or 100 mg/kg b.w. T3, or both substances or the same volume of vehicle (control group) until day 15 of life. The animals were sacrificed on day 50. Morphometric analysis and immunohistochemical reactions were performed using antibodies against Vimentin, Proliferating Cell Nuclear Antigen and Connexin 43 in the testis. Sertoli cell count, efficiency of spermatogenesis, and hormonal pattern were examined. Disturbances in the connexin 43 expression reduced the number of Sertoli cells, the efficiency of spermatogenesis and impaired endocrine function of testes in adult rats treated with FSH and T3 during puberty. Stimulation with FSH alone increased Sertoli cell number, but was associated with a negative effect on cell-to-cell connexin 43-dependent communication, with a consequential reduction of spermatogenesis efficiency. J. Exp. Zool. 323A: 256-265, 2015. © 2015 Wiley Periodicals, Inc.

  19. Habitat planning, maintenance and management working group

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The Gulf of Mexico (GOM), called {open_quotes}America`s Sea,{close_quotes} is actually a small ocean basin covering over 1.5 million square kilometers. Because of the multiple uses, diversity, and size of the Gulf`s resources, management is shared by a number of governmental agencies including the Minerals Management Service, the Gulf of Mexico Fishery Management Council, the Gulf States Marine Fisheries Commission, National Marine Fisheries Service, the US Coast Guard, the US Army Corps of Engineers, and the five Gulf states fisheries agencies. All of these entities share a common goal of achieving optimum sustainable yield to maximize geological, biological, social, and economic benefits from these resources. These entities also share a common theme that the successful management of the northern GOM requires maintenance and enhancement of both the quantity and quality of habitats. A closer look at the GOM shows the sediment to be clearly dominated by vast sand and mud plains. These soft bottom habitats are preferred by many groundfish and shrimp species and, thus, have given rise to large commercial fisheries on these stocks. Hard bottom and reef habitats, on the other hand, are limited to approximately 1.6% of the total area of the Gulf, so that, while there are high demands by commercial and recreational fishermen for reef associated species, the availability of habitat for these stocks is limited. The thousands of oil and gas structures placed in the Gulf have added significant amounts of new hard substrate. The rigs-to-reefs concept was a common sense idea with support from environmental user groups and the petroleum industry for preserving a limited but valuable habitat type. As long as maximizing long-term benefits from the Gulf s resources for the greatest number of users remains the goal, then programs such as Rigs-to-Reefs will remain an important tool for fisheries and habitat managers in the Gulf.

  20. Habitat Evaluation Procedures (HEP) Report; Carey Creek, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    In August 2002, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Carey Creek property, an acquisition completed by the Kalispel Tribe of Indians in December 2001. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Carey Creek Project provides a total of 172.95 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 4.91 HUs for bald eagle, black-capped chickadee, and white-tailed deer. Forested wetlands provide 52.68 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Scrub-shrub wetlands provide 2.82 HUs for mallard, yellow warbler and white-tailed deer. Wet meadow and grassland meadow provide 98.13 HUs for mallard and Canada goose. Emergent wetlands provide 11.53 HUs for mallard, muskrat, and Canada goose. Open water provides 2.88 HUs for Canada goose, mallard, and muskrat. The objective of using HEP at the Carey Creek Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  1. Habitat Use Database - Groundfish Essential Fish Habitat (EFH) Habitat Use Database (HUD)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Habitat Use Database (HUD) was specifically designed to address the need for habitat-use analyses in support of groundfish EFH, HAPCs, and fishing and nonfishing...

  2. Habitat monitoring needs for Arapaho NWR

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document is the refuge's ideas on what level of monitoring is needed for each habitat objective. Habitat objectives include riparian habitat, wetland habitat,...

  3. Parasitism of Lepidopterous Stem Borers in Cultivated and Natural Habitats

    Science.gov (United States)

    Mailafiya, Duna Madu; Le Ru, Bruno Pierre; Kairu, Eunice Waitherero; Dupas, Stéphane; Calatayud, Paul-André

    2011-01-01

    Plant infestation, stem borer density, parasitism, and parasitoid abundance were assessed during two years in two host plants, Zea mays (L.) (Cyperales: Poaceae) and Sorghum bicolor (L.) (Cyperales: Poaceae), in cultivated habitats. The four major host plants (Cyperus spp., Panicum spp., Pennisetum spp., and Sorghum spp.) found in natural habitats were also assessed, and both the cultivated and natural habitat species occurred in four agroecological zones in Kenya. Across habitats, plant infestation (23.2%), stem borer density (2.2 per plant), and larval parasitism (15.0%) were highest in maize in cultivated habitats. Pupal parasitism was not higher than 4.7% in both habitats, and did not vary with locality during each season or with host plant between each season. Cotesia sesamiae (Cameron) and C. flavipes Cameron (Hymenoptera: Braconidae) were the key parasitoids in cultivated habitats (both species accounted for 76.4% of parasitized stem borers in cereal crops), but not in natural habitats (the two Cotesia species accounted for 14.5% of parasitized stem borers in wild host plants). No single parasitoid species exerted high parasitism rates on stem borer populations in wild host plants. Low stem borer densities across seasons in natural habitats indicate that cereal stem borer pests do not necessarily survive the non-cropping season feeding actively in wild host plants. Although natural habitats provided refuges for some parasitoid species, stem borer parasitism was generally low in wild host plants. Overall, because parasitoids contribute little in reducing cereal stem borer pest populations in cultivated habitats, there is need to further enhance their effectiveness in the field to regulate these pests. PMID:21526933

  4. Parasitism of lepidopterous stem borers in cultivated and natural habitats.

    Science.gov (United States)

    Mailafiya, Duna Madu; Le Ru, Bruno Pierre; Kairu, Eunice Waitherero; Dupas, Stéphane; Calatayud, Paul-André

    2011-01-01

    Plant infestation, stem borer density, parasitism, and parasitoid abundance were assessed during two years in two host plants, Zea mays (L.) (Cyperales: Poaceae) and Sorghum bicolor (L.) (Cyperales: Poaceae), in cultivated habitats. The four major host plants (Cyperus spp., Panicum spp., Pennisetum spp., and Sorghum spp.) found in natural habitats were also assessed, and both the cultivated and natural habitat species occurred in four agroecological zones in Kenya. Across habitats, plant infestation (23.2%), stem borer density (2.2 per plant), and larval parasitism (15.0%) were highest in maize in cultivated habitats. Pupal parasitism was not higher than 4.7% in both habitats, and did not vary with locality during each season or with host plant between each season. Cotesia sesamiae (Cameron) and C. flavipes Cameron (Hymenoptera: Braconidae) were the key parasitoids in cultivated habitats (both species accounted for 76.4% of parasitized stem borers in cereal crops), but not in natural habitats (the two Cotesia species accounted for 14.5% of parasitized stem borers in wild host plants). No single parasitoid species exerted high parasitism rates on stem borer populations in wild host plants. Low stem borer densities across seasons in natural habitats indicate that cereal stem borer pests do not necessarily survive the non-cropping season feeding actively in wild host plants. Although natural habitats provided refuges for some parasitoid species, stem borer parasitism was generally low in wild host plants. Overall, because parasitoids contribute little in reducing cereal stem borer pest populations in cultivated habitats, there is need to further enhance their effectiveness in the field to regulate these pests.

  5. Habitats of Life

    Science.gov (United States)

    Dirk, Schulze-Makuch; Irwin, Louis N.

    There are four principal habitats in which life may exist - the surface of a planetary body, its subsurface, its atmosphere and space. From our own experience we know that life does exist on the surface of a planet, in its subsurface, and transiently at least in the atmosphere. Where it is present, it exists in a surprising diversity and in a variety of microhabitats, from deep caverns (Hose et al. 2000, Melim et al. 2001) to hydrothermal fluids and hot springs of various chemistries (Jannasch 1995, Rzonca and Schulze-Makuch 2002), to the frozen deserts of Antarctica (Friedmann 1982, Sun and Friedmann 1999). In this chapter we will elaborate on the principal habitats, the constraints they impose on life, and the possibilities they provide.

  6. FSH-400型风力提水机的设计试验研究%DESIGN TEST AND RESEARCH OF FSH-400 TYPE WIND POWER WATER PUMPING MACHINE

    Institute of Scientific and Technical Information of China (English)

    胡建栋; 邹占武; 徐双信

    2013-01-01

    为了完善我国北方高扬程小流量风力提水机系列,适应不同井深和提水量要求,对已使用过的风力提水机组的结构性能和主要参数进行比较分析.结合牧区多年来推广应用的实践,以新研发的FSH-400型高性能风力提水机为例,进行V-n特性、调速与调向、运行安全与大风自动保护等性能检测.试验表明,该型风力提水机组,风轮采用弧形可变扭角和流线形叶片,优化了风力机的整体性能,能量转换率高,与筒井(机井)匹配性好,造价低廉,无人值守、操作简单、使用可靠.除满足人畜饮水外,结合微滴灌技术,还可用于草场、饲料地灌溉及城乡道路景观绿化、苗木栽培等.%In order to improve the high lift and small flow wind power water pumping machine series in the north of China,adapt to different well depth and water carry conditions,the comparative analysis for several types of structure performance were done and the main parameter of wind power water pumping machine product were developed.Combined with popularization and application practice of pastoral areas for many years,with the new development of FSH-400 type of high performance wind power water pumping machine as an example,do the performance text for V-n characteristics,safety operation,the automatic protection,speed control and direction adjustment.Test showed that this type of wind pumping water equipment' s rotor uses segment variable torsion angle and streamline blade,optimize the overall performance of the wind pumping water machine.It' s energy conversion rate is high,fit well,low cost,unattended operation,simple and reliable operation.In addition to meet people and livestock drinking need,combined with micro drip irrigation technology,it can be used for pasture,feed irrigation,urban and rural road landscape greening,seeding cultivation,etc.

  7. Natural Propagation and Habitat Improvement, Volume I, Oregon, Supplement C, White River Habitat Inventory, 1983 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Heller, David

    1984-04-01

    More than 130 miles of stream fish habitat was inventoried and evaluated on the Mt. Hood National Forest during the first year of this multi-year project. First year tasks included field inventory and evaluation of habitat conditions on the White River and tributary streams thought to have the highest potential for supporting anadromous fish populations. All streams inventoried were located on the Mt. Hood National Forest. The surveyed area appears to contain most of the high quality anadromous fish habitat in the drainage. Habitat conditions appear suitable for steelhead, coho, and chinook salmon, and possibly sockeye. One hundred and twenty-four miles of potential anadromous fish habitat were identifed in the survey. Currently, 32 miles of this habitat would be readily accessible to anadromous fish. An additional 72 miles of habitat could be accessed with only minor passage improvement work. About 20 miles of habitat, however, will require major investment to provide fish passage. Three large lakes (Boulder, 14 acres; Badger, 45 acres; Clear, 550 acres) appear to be well-suited for rearing anadromous fish, although passage enhancement would be needed before self-sustaining runs could be established in any of the lakes.

  8. Influence of FSH and hCG on the resumption of meiosis of bovine oocytes surrounded by cumulus cells connected to membrana granulosa.

    Science.gov (United States)

    van Tol, H T; van Eijk, M J; Mummery, C L; van den Hurk, R; Bevers, M M

    1996-10-01

    Cumulus oocyte complexes (COCs) and cumulus oocyte complexes connected to a piece of the membrane granulosa (COCGs) were isolated from bovine antral follicles with a diameter of 2 to 8 mm. After culture of COCGs without gonadotrophic hormones for 22 hr approximately 50% of the oocytes were still in the germinal vesicle (GV) stage. Histology of the COCGs showed that the pieces of the membrana granulosa were free of thecal cells and parts of the basal membrane. This indicates that the membrana granulosa solely inhibits the progression of meiosis. To investigate the effect of gonadotropins on the resumption of meiosis of oocytes from small and medium sized antral follicles, COCs and COCGs were cultured with or without rec-hFSH or hCG. Addition of 0.05 IU rec-hFSH to the culture medium of COCGs resulted in germinal vesicle breakdown in 97.8% of the oocytes compared to 46% in the control group, and an increase of the diameter of the COCs (479 microns vs. 240 microns in the control group). Addition of 0.05 IU hCG to the culture medium had no effect on nuclear maturation (47.2% GV vs. 48.5% GV in the control group) nor on cumulus expansion (246 microns vs. 240 microns in the control group). RT-PCR on cDNA of the follicular wall, cumulus cells, granulosa cells, COCs, and oocytes revealed that mRNA for FSH receptor was present in all cell types except oocytes. mRNA of the LH receptor was detected exclusively in thecal cells. Nucleotide sequence analysis and alignment of the cloned PCR products showed the presence of two isoforms of the FSH receptor mRNA and two isoforms of the LH receptor mRNA. It is concluded that, in vitro, resumption of meiosis of oocytes, originating from small and medium sized antral follicles and meiotically arrested by the membrana granulosa, is triggered by FSH and not by LH. This is supported by the fact that receptors for FSH, but not for LH, are transcribed in the cumulus and granulosa cells of these follicles.

  9. Predictive factors of ovarian response and clinical outcome after IVF/ICSI following a rFSH/GnRH antagonist protocol with or without oral contraceptive pre-treatment

    DEFF Research Database (Denmark)

    Andersen, A Nyboe; Witjes, H; Gordon, K

    2011-01-01

    Prediction of ovarian response prior to the first controlled ovarian stimulation (COS) cycle is useful in determining the optimal starting dose of recombinant FSH (rFSH). However, potentially predictive factors may be subject to inter-cycle variability and many patients are pre-treated with oral...... contraceptives (OC) for scheduling purposes. Our objective was to determine predictive factors of ovarian response for patients undergoing COS with rFSH in a gonadotrophin-releasing hormone antagonist protocol and to determine the inter-cycle variability of these factors....

  10. Predictive factors of ovarian response and clinical outcome after IVF/ICSI following a rFSH/GnRH antagonist protocol with or without oral contraceptive pre-treatment

    DEFF Research Database (Denmark)

    Andersen, A Nyboe; Witjes, H; Gordon, K

    2011-01-01

    Prediction of ovarian response prior to the first controlled ovarian stimulation (COS) cycle is useful in determining the optimal starting dose of recombinant FSH (rFSH). However, potentially predictive factors may be subject to inter-cycle variability and many patients are pre-treated with oral ...... contraceptives (OC) for scheduling purposes. Our objective was to determine predictive factors of ovarian response for patients undergoing COS with rFSH in a gonadotrophin-releasing hormone antagonist protocol and to determine the inter-cycle variability of these factors....

  11. Effect of the FSH receptor single nucleotide polymorphisms (FSHR 307/680) on the follicular fluid hormone profile and the granulosa cell gene expression in human small antral follicles

    DEFF Research Database (Denmark)

    Borgbo, Tanni Kjær; Jeppesen, J V; Lindgren, I

    2014-01-01

    ), by evaluating the hormone and gene expression profiles of human small antral follicles collected under physiological conditions in connection with fertility preservation. In total 69 women at various time during the menstrual cycle were included in this study. The intrafollicular hormone content of 179......The most pronounced effects of FSH signalling are potentially displayed in the follicle fluid, which acts as a reservoir for FSH-induced granulosa cell (GC) secreted hormones. This study investigates the effects of two common polymorphisms of FSHR, FSHR 307 (rs6165) and FSHR 680 (rs6166...... small antral follicles collected under physiological FSH conditions....

  12. Plant Habitat (PH)

    Science.gov (United States)

    Onate, Bryan

    2016-01-01

    The International Space Station (ISS) will soon have a platform for conducting fundamental research of Large Plants. Plant Habitat (PH) is designed to be a fully controllable environment for high-quality plant physiological research. PH will control light quality, level, and timing, temperature, CO2, relative humidity, and irrigation, while scrubbing ethylene. Additional capabilities include leaf temperature and root zone moisture and oxygen sensing. The light cap will have red (630 nm), blue (450 nm), green (525 nm), far red (730 nm) and broad spectrum white LEDs. There will be several internal cameras (visible and IR) to monitor and record plant growth and operations.

  13. Habitat Evaluation Procedures (HEP) Report : West Beaver Lake, 2004-2005 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On September 7, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the West Beaver Lake property, an acquisition completed by the Kalispel Tribe of Indians in September 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, mallard, muskrat, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The West Beaver Lake Project provides a total of 103.08 Habitat Units (HUs) for the species evaluated. Emergent wetland habitat provides 7.17 HUs for mallard and muskrat. Conifer forest habitat provides 95.91 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. The objective of using HEP at the West Beaver Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  14. Vacant habitats in the Universe.

    Science.gov (United States)

    Cockell, Charles S

    2011-02-01

    The search for life on other planets usually makes the assumption that where there is a habitat, it will contain life. On the present-day Earth, uninhabited habitats (or vacant habitats) are rare, but might occur, for example, in subsurface oils or impact craters that have been thermally sterilized in the past. Beyond Earth, vacant habitats might similarly exist on inhabited planets or on uninhabited planets, for example on a habitable planet where life never originated. The hypothesis that vacant habitats are abundant in the Universe is testable by studying other planets. In this review, I discuss how the study of vacant habitats might ultimately inform an understanding of how life has influenced geochemical conditions on Earth. Copyright © 2010 Elsevier Ltd. All rights reserved.

  15. PIVET rFSH dosing algorithms for individualized controlled ovarian stimulation enables optimized pregnancy productivity rates and avoidance of ovarian hyperstimulation syndrome

    Directory of Open Access Journals (Sweden)

    Yovich JL

    2016-08-01

    Full Text Available John L Yovich,1,2,* Birgit Alsbjerg,3,4,* Jason L Conceicao,1 Peter M Hinchliffe,1 Kevin N Keane1,2,* 1PIVET Medical Centre, Perth, 2School of Biomedical Science, Curtin Health Innovation Research Institute Bioscience, Curtin University, Perth, WA, Australia; 3The Fertility Clinic, Skive Regional Hospital, Skive, 4Faculty of Health, Aarhus University, Aarhus, Denmark *These authors contributed equally to this work Abstract: The first PIVET algorithm for individualized recombinant follicle stimulating hormone (rFSH dosing in in vitro fertilization, reported in 2012, was based on age and antral follicle count grading with adjustments for anti-Müllerian hormone level, body mass index, day-2 FSH, and smoking history. In 2007, it was enabled by the introduction of a metered rFSH pen allowing small dosage increments of ~8.3 IU per click. In 2011, a second rFSH pen was introduced allowing more precise dosages of 12.5 IU per click, and both pens with their individual algorithms have been applied continuously at our clinic. The objective of this observational study was to validate the PIVET algorithms pertaining to the two rFSH pens with the aim of collecting ≤15 oocytes and minimizing the risk of ovarian hyperstimulation syndrome. The data set included 2,822 in vitro fertilization stimulations over a 6-year period until April 2014 applying either of the two individualized dosing algorithms and corresponding pens. The main outcome measures were mean oocytes retrieved and resultant embryos designated for transfer or cryopreservation permitted calculation of oocyte and embryo utilization rates. Ensuing pregnancies were tracked until live births, and live birth productivity rates embracing fresh and frozen transfers were calculated. Overall, the results showed that mean oocyte numbers were 10.0 for all women <40 years with 24% requiring rFSH dosages <150 IU. Applying both specific algorithms in our clinic meant that the starting dose was not altered for

  16. Use of purified FSH and LH for embryo production, cryopreservation by conventional freezing or vitrification and transfer of embryos in dairy ewes

    Directory of Open Access Journals (Sweden)

    Giovanni Martemucci

    2010-01-01

    Full Text Available Three experiments were carried out with the aim of evaluating the efficiency of techniques of in vivo production, storageand transfer of embryos in dairy sheep. Experiment I - For embryo production, thirty-one ewes were synchronized withFGA (vaginal sponges, 40 mg, 9 d and PGF2α (ICI; 50 μg, 7th d, and subdivided into three groups corresponding to thefollowing superovulatory treatments over 3 days with purified gonadotrophic preparations: A control, FSH/LH ratio = 1(250 IU p-FSH : 250 UI p-LH; B FSH/LH ratio = 2 (250 IU p-FSH : 125 IU p-LH and daily FSH/LH ratio of 3.4 – 1.7 –0.8 in the 3 days of treatment, respectively; C FSH/LH ratio = 2 (250 IU p-FSH : 125 IU p-LH and daily FSH/LH ratioof 5.0 – 1.0 – 0.3. On the 7th day after oestrus and mating, ovarian response and embryo production were evaluated.Experiment II – Three freezing methods were evaluated based upon post-thaw embryo quality: CF conventional slowfreezing by 1.5 M ethylene glycol (EG; V-1 one-step vitrification based on exposure of the embryos to one solution (EG7.15 M + ficoll 2.5 mM; V-3 vitrification in three steps, corresponding to three solutions at increasing concentration ofglycerol (GLY and EG (GLY 1.4 M; GLY 3.4 M + EG 1.4 M; GLY 4.6 M + EG 3.4 M. V-1 and V-3 frozen embryos weredirectly plunged in liquid nitrogen. At thawing, embryo viability was evaluated on the basis of morphological features.Experiment III – For embryo transfer, a total of 26 recipient ewes were synchronized with donors. On the 7th d fromoestrus, 11 recipient ewes received fresh embryos (Group FE – control and 15 recipients received vitrified-thawedembryos (Group VTE. Each recipient received 2 embryos. Superovulatory treatment B significantly advanced the onsetof oestrus compared to the control (27.3 vs 34.7 h; P10.8. Transferable embryos in Group B (7.2 resulted similar to Group A (5.3 and significantly (Pcompared to Group C (3.2. V3-method resulted in the highest (PCF- and V1-methods

  17. A new way of setting rFSH deposit: a case of severe injection error in IVF/ICSI cycle ending with live birth

    Science.gov (United States)

    Mayer, Richard Bernhard; Ebner, Thomas; Shebl, Omar; Tews, Gernot

    2012-01-01

    We present a case with a severe injection error: a 25- year old woman with secondary infertility caused by a male factor was enrolled in our IVF/ICSI-ET program. Stimulation was performed in a long- protocol and ovarian stimulation, using rFSH follitropin beta, starting on the third day of the menstrual cycle. The rFSH dose per day was 900 IU-0 IU-0 IU-0 IU. Due to normal ovarian response and follicle growth, stimulation was continued and there was no detriment in oocyte quality and no symptoms of OHSS. Following blastocyte transfer cesarean section was unpreventable at 37+5 weeks of gestation due to an impacted transverse lie. Different stimulation protocols are needed for appropriate treatment of various patients provided that the administration of treatment was done correctly. In the case of injection errors, continuing stimulation protocol seems to be achievable in certain cases considering hormone levels and the process of follicle growth. PMID:24592042

  18. Introduction to stream network habitat analysis

    Science.gov (United States)

    Bartholow, John M.; Waddle, Terry J.

    1986-01-01

    Increasing demands on stream resources by a variety of users have resulted in an increased emphasis on studies that evaluate the cumulative effects of basinwide water management programs. Network habitat analysis refers to the evaluation of an entire river basin (or network) by predicting its habitat response to alternative management regimes. The analysis principally focuses on the biological and hydrological components of the riv er basin, which include both micro- and macrohabitat. (The terms micro- and macrohabitat are further defined and discussed later in this document.) Both conceptual and analytic models are frequently used for simplifying and integrating the various components of the basin. The model predictions can be used in developing management recommendations to preserve, restore, or enhance instream fish habitat. A network habitat analysis should begin with a clear and concise statement of the study objectives and a thorough understanding of the institutional setting in which the study results will be applied. This includes the legal, social, and political considerations inherent in any water management setting. The institutional environment may dictate the focus and level of detail required of the study to a far greater extent than the technical considerations. After the study objectives, including species on interest, and institutional setting are collectively defined, the technical aspects should be scoped to determine the spatial and temporal requirements of the analysis. A macro level approach should be taken first to identify critical biological elements and requirements. Next, habitat availability is quantified much as in a "standard" river segment analysis, with the likely incorporation of some macrohabitat components, such as stream temperature. Individual river segments may be aggregated to represent the networkwide habitat response of alternative water management schemes. Things learned about problems caused or opportunities generated may

  19. Time course and role of LH and FSH in the expansion of the Leydig cell population at the time of puberty in the rhesus monkey (Macaca mulatta)

    Science.gov (United States)

    Verhagen, I.; Ramaswamy, S.; Teerds, K.J.; Keijer, J.; Plant, T. M.

    2014-01-01

    In higher primates development of the adult population of Leydig cells has received little attention. Here, the emergence of 3β-hydroxysteroid dehydrogenase (HSD3B) positive cells in the testis of the rhesus monkey was examined during spontaneous puberty, and correlated with S-phase labeling in the interstitium at this critical stage of development. In addition, the relative role of LH and FSH in initiating the pubertal expansion of Leydig cells was studied by precociously stimulating the juvenile testis in vivo with pulsatile 11-day infusions of recombinant LH and FSH, either alone or in combination. At the time of castration, testes were immersion fixed in Bouin’s, embedded in paraffin and sectioned at 5 μm. Leydig cells/testis were enumerated using HSD3B as a Leydig cell marker. Leydig cell number per testis increased progressively during puberty to reach values in the adult approximately 10 fold greater than in early pubertal animals. The rise in cell number was associated with an increase in nuclear diameter. That the pubertal expansion of Leydig cell number was driven primarily by the increase in LH secretion at this stage of development was suggested by the finding that precocious stimulation of mid juvenile monkeys with LH, either alone or in combination with that of FSH, resulted in a 20 to 30 fold increase in the number of HSD3B positive cells. Interestingly, precocious FSH stimulation, alone, also resulted in appearance of Leydig cells as indicated by the occasional HSD3B positive cell in the interstitium. The nuclear diameter of these Leydig cells, however was less than that of those generated in response to LH. PMID:25269763

  20. Recombinant FSH Compared to Clomiphene Citrate as the First-Line in Ovulation Induction in Polycystic Ovary Syndrome Using Newly Designed Pens: A Randomized Controlled Trial

    Directory of Open Access Journals (Sweden)

    Batool Hossein-Rashidi

    2016-05-01

    Full Text Available Objective: Since there is still controversy regarding the best first-line choice for ovulation induction (OI other than clomiphene citrate (CC in infertile women diagnosed with polycystic ovary syndrome (PCOS, the aim of the present study was to compare recombinant human FSH with CC as the first course of OI in these women.Materials and methods: In this pilot randomized controlled trial, 104 infertile women diagnosed with PCOS were randomized in two groups to receive either CC with the dose of 100mg per day from day 3 of a spontaneous or progestin-induced menstruation for 5 days or rFSH with the starting dose of 50 IU daily {and weekly dose increment of as low as 12.5 IU}, on the day4 of the cycle. They were assessed during a single OI course. The pregnancy rate (PR and live birth rate (LBR were the primary outcomes. The follicular response, endometrial thickness, cancellation of the cycles and ovarian hyper stimulation (OHSS rate were the secondary outcomes.Results: Analyzing data of 96 patients using Chi2 and Fischer’s Exact test (44 in rFSH group and 52 in CC group, both PR and LBR were comparable in the two groups {13.6% vs. 9.6% and 11.4% vs. 9.6% respectively}, with the difference not to be significant (p > 0.05. No cases of OHSS or multiple gestations happened during the treatment course.Conclusion: It seems that rFSH is as efficacious as CC while not with more complications for the first-line OI in infertile women with PCOS. However, due to the limitations of the present study including the small population and the single cycle of treatment, our results did not come out to prove this and more studies with larger study population are needed to compare the cumulative PR and LBR.

  1. The adapter protein APPL1 links FSH receptor to inositol 1,4,5-trisphosphate production and is implicated in intracellular Ca(2+) mobilization.

    Science.gov (United States)

    Thomas, Richard M; Nechamen, Cheryl A; Mazurkiewicz, Joseph E; Ulloa-Aguirre, Alfredo; Dias, James A

    2011-04-01

    FSH binds to its receptor (FSHR) on target cells in the ovary and testis, to regulate oogenesis and spermatogenesis, respectively. The signaling cascades activated after ligand binding are extremely complex and have been shown to include protein kinase A, mitogen-activated protein kinase, phosphatidylinositol 3-kinase/protein kinase B, and inositol 1,4,5-trisphosphate-mediated calcium signaling pathways. The adapter protein APPL1 (Adapter protein containing Pleckstrin homology domain, Phosphotyrosine binding domain and Leucine zipper motif), which has been linked to an assortment of other signaling proteins, was previously identified as an interacting protein with FSHR. Thus, alanine substitution mutations in the first intracellular loop of FSHR were generated to determine which residues are essential for FSHR-APPL1 interaction. Three amino acids were essential; when any one of them was altered, APPL1 association with FSHR mutants was abrogated. Two of the mutants (L377A and F382A) that displayed poor cell-surface expression were not studied further. Substitution of FSHR-K376A did not affect FSH binding or agonist-stimulated cAMP production in either transiently transfected human embryonic kidney cells or virally transduced human granulosa cells (KGN). In the KGN line, as well as primary cultures of rat granulosa cells transduced with wild type or mutant receptor, FSH-mediated progesterone or estradiol production was not affected by the mutation. However, in human embryonic kidney cells inositol 1,4,5-trisphosphate production was curtailed and KGN cells transduced with FSHR-K376A evidenced reduced Ca(2+) mobilization from intracellular stores after FSH treatment.

  2. Ecological values of shallow-water habitats: Implications for the restoration of disturbed ecosystems

    Science.gov (United States)

    Lopez, C.B.; Cloern, J.E.; Schraga, T.S.; Little, A.J.; Lucas, L.V.; Thompson, J.K.; Burau, J.R.

    2006-01-01

    A presumed value of shallow-habitat enhanced pelagic productivity derives from the principle that in nutrient-rich aquatic systems phytoplankton growth rate is controlled by light availability, which varies inversely with habitat depth. We measured a set of biological indicators across the gradient of habitat depth within the Sacramento-San Joaquin River Delta (California) to test the hypothesis that plankton biomass, production, and pelagic energy flow also vary systematically with habitat depth. Results showed that phytoplankton biomass and production were only weakly related to phytoplankton growth rates whereas other processes (transport, consumption) were important controls. Distribution of the invasive clam Corbicula fluminea was patchy, and heavily colonized habitats all supported low phytoplankton biomass and production and functioned as food sinks. Surplus primary production in shallow, uncolonized habitats provided potential subsidies to neighboring recipient habitats. Zooplankton in deeper habitats, where grazing exceeded phytoplankton production, were likely supported by significant fluxes of phytoplankton biomass from connected donor habitats. Our results provide three important lessons for ecosystem science: (a) in the absence of process measurements, derived indices provide valuable information to improve our mechanistic understanding of ecosystem function and to benefit adaptive management strategies; (b) the benefits of some ecosystem functions are displaced by water movements, so the value of individual habitat types can only be revealed through a regional perspective that includes connectedness among habitats; and (c) invasive species can act as overriding controls of habitat function, adding to the uncertainty of management outcomes. ?? 2006 Springer Science+Business Media, Inc.

  3. The effects of levonorgestrel on FSH-stimulated primary rat granulosa cell cultures through gene expression profiling are associated to hormone and folliculogenesis processes.

    Science.gov (United States)

    Lira-Albarrán, Saúl; Larrea-Schiavon, Marco F; González, Leticia; Durand, Marta; Rangel, Claudia; Larrea, Fernando

    2017-01-05

    Levonorgestrel (LNG), a synthetic progestin, is used in emergency contraception (EC). The mechanism is preventing or delaying ovulation at the level of the hypothalamic pituitary unit; however, little knowledge exists on LNG effects at the ovary. The aim of this study was to identify the effects of LNG on FSH-induced 17β-estradiol (E2) production, including LNG-mediated changes on global gene expression in rat granulosa cells (GC). Isolated GC from female Wistar rats were incubated in vitro in the presence or absence of human FSH and progestins. At the end of incubations, culture media and cells were collected for E2 and mRNA quantitation. The results showed the ability of LNG to inhibit both hFSH-induced E2 production and aromatase gene expression. Microarray analysis revealed that LNG treatment affects GC functionality particularly that related to folliculogenesis and steroid metabolism. These results may offer additional evidence for the mechanisms of action of LNG as EC. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  4. Concomitant use of FSH and low-dose recombinant hCG during the late follicular phase versus conventional controlled ovarian stimulation for intracytoplasmic sperm injection cycles.

    Science.gov (United States)

    Iaconelli, Carla Andrade Rebello; Setti, Amanda Souza; Braga, Daniela Paes Almeida Ferreira; Maldonado, Luiz Guilherme Louzada; Iaconelli, Assumpto; Borges, Edson; Aoki, Tsutomu

    2017-03-22

    The objective of this study was to investigate the effects of low-dose hCG supplementation on ICSI outcomes and controlled ovarian stimulation (COS) cost. Three hundred and thirty patients undergoing ICSI were split into groups according to the COS protocol: (i) control group (n = 178), including patients undergoing conventional COS treatment; and (ii) low-dose hCG group (n = 152), including patients undergoing COS with low-dose hCG supplementation. Lower mean total doses of FSH administered and higher mean oestradiol level and mature oocyte rates were observed in the low-dose hCG group. A significantly higher fertilization rate, high-quality embryo rate and blastocyst formation rate were observed in the low-dose hCG group as compared to the control group. The miscarriage rate was significantly higher in the control group compared to the low-dose hCG group. A significantly lower incidence of OHSS was observed in the low-dose hCG group. There was also a significantly lower gonadotropin cost in the low-dose hCG group as compared to the control group ($1235.0 ± 239.0×$1763.0 ± 405.3, p hCG and FSH results in a lower abortion rate and increased number of mature oocytes retrieved, as well as improved oocyte quality, embryo quality and blastocyst formation and reduced FSH requirements.

  5. Changes in Habitat and Populations of Steelhead Trout, Coho Salmon, and Chinook Salmon in Fish Creek, Oregon; Habitat Improvement, 1983-1987 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Everest, Fred H. (Oregon State University, Pacific Northwest Forest and Range Experiment Station, Corvallis, OR); Hohler, David B.; Cain, Thomas C. (Mount Hood National Forest, Clackamas River Ranger District, Estacada, OR)

    1988-03-01

    Construction and evaluation of salmonid habitat improvements on Fish Creek, a tributary of the upper Clackamas River, began in 1982 as a cooperative venture between the Estacada Ranger District, Mt. Hood National Forest, and the Anadromous Fish Habitat Research Unit of the Pacific Northwest Research Station (PNW), USDA Forest Service. The project was initially conceived as a 5-year effort (1982-1987) to be financed with Forest Service funds. The habitat improvement program and the evaluation of improvements were both expanded in mid-1983 when the Bonneville Power Administration (BPA) entered into an agreement with the Mt. Hood National Forest to cooperatively fund work on Fish Creek. Habitat improvement work in the basin is guided by the Fish Creek Habitat Rehabilitation-Enhancement Framework developed cooperatively by the Estacada Ranger District, the Oregon Department of Fish and Wildlife, and the Pacific Northwest Research Station. The framework examines potential factors limiting production of salmonids in the basin, and the appropriate habitat improvement measures needed to address the limiting factors. Habitat improvement work in the basin has been designed to: (1) improve quantity, quality, and distribution of spawning habitat for coho and spring chinook salmon and steelhead trout, (2) increase low flow rearing habitat for steelhead trout and coho salmon, (3) improve overwintering habitat for coho salmon and steelhead trout, (4) rehabilitate riparian vegetation to improve stream shading to benefit all species, and (5) evaluate improvement projects from a drainage wide perspective. The objectives of the evaluation include: (1) Drainage-wide evaluation and quantification of changes in salmonid spawning and rearing habitat resulting from a variety of habitat improvements. (2) Evaluation and quantification of changes in fish populations and biomass resulting from habitat improvements. (3) Benefit-cost analysis of habitat improvements.

  6. Coeur d'Alene Tribe Fish and Wildlife Program Habitat Protection Plan; Implementation of Fisheries Enhancement Opportunities on the Coeur d'Alene Reservation, 1997-2002 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Vitale, Angelo; Roberts, Frank; Peters, Ronald

    2002-06-01

    Throughout the last century, the cumulative effects of anthropogenic disturbances have caused drastic watershed level landscape changes throughout the Reservation and surrounding areas (Coeur d'Alene Tribe 1998). Changes include stream channelization, wetland draining, forest and palouse prairie conversion for agricultural use, high road density, elimination of old growth timber stands, and denuding riparian communities. The significance of these changes is manifested in the degradation of habitats supporting native flora and fauna. Consequently, populations of native fish, wildlife, and plants, which the Tribe relies on as subsistence resources, have declined or in some instances been extirpated (Apperson et al. 1988; Coeur d'Alene Tribe 1998; Lillengreen et al. 1996; Lillengreen et al. 1993; Gerry Green Coeur d'Alene Tribe wildlife Biologist, personal communication 2002). For example, bull trout (Salvelinus confluentus) are not present at detectable levels in Reservation tributaries, westslope cutthroat trout (Oncorhynchus clarki lewisi) are not present in numbers commensurate with maintaining harvestable fisheries (Lillengreen et al. 1993, 1996), and the Sharp-tailed grouse (Tympanuchus phasianellus) are not present at detectable levels on the Reservation (Gerry Green, Coeur d'Alene Tribe wildlife biologist, personal communication). The Coeur d'Alene Tribe added Fisheries and Wildlife Programs to their Natural Resources Department to address these losses and protect important cultural, and subsistence resources for future generations. The Tribal Council adopted by Resolution 89(94), the following mission statement for the Fisheries Program: 'restore, protect, expand and re-establish fish populations to sustainable levels to provide harvest opportunities'. This mission statement, focused on fisheries restoration and rehabilitation, is a response to native fish population declines throughout the Tribe's aboriginal territory

  7. Clay Animals and Their Habitats

    Science.gov (United States)

    Adamson, Kay

    2010-01-01

    Creating clay animals and their habitats with second-grade students has long been one of the author's favorite classroom activities. Students love working with clay and they also enjoy drawing animal homes. In this article, the author describes how the students created a diorama instead of drawing their clay animal's habitat. This gave students…

  8. Habitat specialization through germination cueing

    DEFF Research Database (Denmark)

    Ten Brink, Dirk-Jan; Hendriksma, Harmen; Bruun, Hans Henrik

    2013-01-01

    This study examined the adaptive association between seed germination ecology and specialization to either forest or open habitats across a range of evolutionary lineages of seed plants, in order to test the hypotheses that (1) species' specialization to open vs. shaded habitats is consistently...

  9. Habitat modeling for biodiversity conservation.

    Science.gov (United States)

    Bruce G. Marcot

    2006-01-01

    Habitat models address only 1 component of biodiversity but can be useful in addressing and managing single or multiple species and ecosystem functions, for projecting disturbance regimes, and in supporting decisions. I review categories and examples of habitat models, their utility for biodiversity conservation, and their roles in making conservation decisions. I...

  10. The FSHB -211G>T variant attenuates serum FSH levels in the supraphysiological gonadotropin setting of Klinefelter syndrome.

    Science.gov (United States)

    Busch, Alexander S; Tüttelmann, Frank; Zitzmann, Michael; Kliesch, Sabine; Gromoll, Jörg

    2015-05-01

    Klinefelter syndrome (47, XXY) is the most frequent genetic cause of male infertility and individuals share the endocrine hallmark of hypergonadotropic hypogonadism. Single-nucleotide polymorphisms located within the FSHB/FSHR gene were recently shown to impact serum follicle-stimulating hormone (FSH) levels and other reproductive parameters in men. The objective of this study was to analyse the effect of FSHB-211G>T (c.-280G>T, rs10835638) as well as FSHR c.2039G>A (rs6166) and FSHR c.-29G>A (rs1394205) on endocrine and reproductive parameters in untreated and testosterone-treated Klinefelter patients. Patients were retrospectively selected from the clientele attending a university-based andrology centre. A total of 309 non-mosaic Klinefelter individuals between 18 and 65 years were included and genotyped for the variants by TaqMan assays. The untreated group comprised 248 men, in which the FSHB -211G>T allele was significantly associated with the reduced serum follicle-stimulating hormone levels (-6.5 U/l per T allele, P=1.3 × 10(-3)). Testosterone treatment (n=150) abolished the observed association. When analysing patients before and under testosterone treatment (n=89), gonadotropin levels were similarly suppressed independently of the FSHB genotype. The FSHR polymorphisms did not exhibit any significant influence in any group, neither on the endocrine nor reproductive parameters. In conclusion, a hypergonadotropic setting such as Klinefelter syndrome does not mask the FSHB -211G>T genotype effects on the follicle-stimulating hormone serum levels. The impact was indeed more pronounced compared with normal or infertile men, whereas gonadotropin suppression under testosterone treatment seems to be independent of the genotype. Thus, the FSHB -211G>T genotype is a key determinant in the regulation of gonadotropins in different reproductive-endocrine pathopyhsiologies.

  11. Habitat association of Klebsiella species.

    Science.gov (United States)

    Bagley, S T

    1985-02-01

    The genus Klebsiella is seemingly ubiquitous in terms of its habitat associations. Klebsiella is a common opportunistic pathogen for humans and other animals, as well as being resident or transient flora (particularly in the gastrointestinal tract). Other habitats include sewage, drinking water, soils, surface waters, industrial effluents, and vegetation. Until recently, almost all these Klebsiella have been identified as one species, ie, K. pneumoniae. However, phenotypic and genotypic studies have shown that "K. pneumoniae" actually consists of at least four species, all with distinct characteristics and habitats. General habitat associations of Klebsiella species are as follows: K. pneumoniae--humans, animals, sewage, and polluted waters and soils; K. oxytoca--frequent association with most habitats; K. terrigena--unpolluted surface waters and soils, drinking water, and vegetation; K. planticola--sewage, polluted surface waters, soils, and vegetation; and K. ozaenae/K. rhinoscleromatis--infrequently detected (primarily with humans).

  12. Habitat Evaluation Procedures (HEP) Report; Calispell Creek Project, Technical Report 2004-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On July 13, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Calispell Creek property, an acquisition completed by the Kalispel Tribe of Indians in February 2004. Evaluation species and appropriate models include Canada goose, mallard, muskrat, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Calispell Creek Project provides a total of 138.17 Habitat Units (HUs) for the species evaluated. Emergent wetland habitat provides 5.16 HUs for mallard and muskrat. Grassland provides 132.02 HUs for mallard and Canada goose. Scrub-shrub vegetation provides 0.99 HUs for yellow warbler and white-tailed deer. The objective of using HEP at the Calispell Creek Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  13. Robotic Precursor Missions for Mars Habitats

    Science.gov (United States)

    Huntsberger, Terry; Pirjanian, Paolo; Schenker, Paul S.; Trebi-Ollennu, Ashitey; Das, Hari; Joshi, Sajay

    2000-07-01

    Infrastructure support for robotic colonies, manned Mars habitat, and/or robotic exploration of planetary surfaces will need to rely on the field deployment of multiple robust robots. This support includes such tasks as the deployment and servicing of power systems and ISRU generators, construction of beaconed roadways, and the site preparation and deployment of manned habitat modules. The current level of autonomy of planetary rovers such as Sojourner will need to be greatly enhanced for these types of operations. In addition, single robotic platforms will not be capable of complicated construction scenarios. Precursor robotic missions to Mars that involve teams of multiple cooperating robots to accomplish some of these tasks is a cost effective solution to the possible long timeline necessary for the deployment of a manned habitat. Ongoing work at JPL under the Mars Outpost Program in the area of robot colonies is investigating many of the technology developments necessary for such an ambitious undertaking. Some of the issues that are being addressed include behavior-based control systems for multiple cooperating robots (CAMPOUT), development of autonomous robotic systems for the rescue/repair of trapped or disabled robots, and the design and development of robotic platforms for construction tasks such as material transport and surface clearing.

  14. Audubon NWR complex annual habitat work plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for...

  15. Steelhead Critical Habitat, Coast - NOAA [ds122

    Data.gov (United States)

    California Department of Resources — This layer depicts areas designated for Steelhead Critical Habitat as well as habitat type and quality in the Coastal California Steelhead ESUs (evolutionarily...

  16. Guam and the Northern Mariana Islands ESI: HABITATS (Habitat Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for benthic marine habitats and plants in Guam and the Northern Mariana Islands. Vector polygons in this...

  17. Riparian Habitat - Product of 2 riparian habitat workshops

    Data.gov (United States)

    California Department of Resources — In two riparian habitat workshops held between 2001 and 2002, scientists and managers identified the need for determining the scope of a consistent and acceptable...

  18. Southeast Alaska ESI: HABITATS (Habitat and Plant Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains biological resource data for seagrass habitats in Southeast Alaska. Vector polygons in this data set represent locations of seagrass...

  19. HabitatSpace: Multidimensional Characterization of Pelagic Essential Fish Habitat

    Science.gov (United States)

    2010-06-01

    That area should be described in terms of ecological characteristics including biological, physical, and chemical parameters, location, and time... Ecologically , essential habitat includes structure or substrate that focuses distribution (e.g., coral reefs, marshes, or kelp beds) and other...feature. For example, seagrass (Fig. 1) is a typical habitat. It grows on the seafloor and is basically a 2-D substrate. Fish that rely on this

  20. Habitat Evaluation Procedures (HEP) Report : Ladd Marsh, 2001 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Oregon Department of Fish and Wildlife

    2001-10-01

    ]) have been working together since 1991 to coordinate the planning, selection, and implementation of BPA-funded wildlife mitigation projects. In 1997, the Oregon wildlife managers developed a programmatic project for mitigation planning and implementation within Oregon. The Ladd Marsh Wildlife Area Additions project is one of many habitat acquisition and restoration projects proposed under the Oregon wildlife managers programmatic project that have been approved and recommended for funding by the NWPPC. The Ladd Marsh Wildlife Area Additions mitigation project will protect and restore wetland, riparian and other habitats on newly acquired parcels at ODFW's Ladd Marsh Wildlife Area (LMWA). Wildlife habitat values resulting from the acquisition and enhancement of Ladd Marsh Wildlife Area lands will contribute towards mitigating for habitat lost as a result of the development and operation of the Columbia Basin hydropower system. This report summarizes the HEP survey conducted in June 2001 to document the baseline habitat values on four parcels recently added to the Ladd Marsh Wildlife Area: the 309.66-acre Wallender property, the 375.54-acre Simonis property, the 161.07-acre Conley Lake property, and the 74.55-acre Becker property. The 2001 HEP Team was comprised of the following members and agencies: Susan Barnes (ODFW), Allen Childs (CTUIR), Tracy Hames (Yakama Indian Nation), Dave Larson (ODFW), Cathy Nowak (Cat Tracks Wildlife Consulting), and Ken Rutherford (ODFW). Results of the HEP will be used to (1) determine the pre-restoration habitat values of the project sites, (2) the number of Habitat Units to be credited to BPA for protection of habitats within the project area, (3) determine the enhancement potential of the sites, and (4) develop a habitat management plan for the area.

  1. Bird communities of natural and modified habitats in Panama

    Science.gov (United States)

    Petit, L.J.; Petit, D.R.; Christian, D.G.; Powell, H.D.W.

    1999-01-01

    enhancement of agricultural and developed lands as wildlife habitat. To understand the true conservation value of these modified lands will require examination not only of numbers but also of the types of species supported by these habitats, their reproductive output and survival rates.

  2. Tidal Creek Sentinel Habitat Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Ecological Research, Assessment and Prediction's Tidal Creeks: Sentinel Habitat Database was developed to support the National Oceanic and Atmospheric...

  3. Hawksbill Sea Turtle Critical Habitat

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for hawksbill turtle as designated by Federal Register Vol. 63, No. 46701, September 2, 1998, Rules and Regulations....

  4. Leatherback Sea Turtle Critical Habitat

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data represent the critical habitat for leatherback turtle as designated by Federal Register Vol. 44, No. 17711, March 23, 1979, Rules and Regulations....

  5. Endangered Species Act Critical Habitat

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Critical habitat (CH) is designated for the survival and recovery of species listed as threatened or endangered under the Endangered Species Act (ESA). Critical...

  6. Waterfowl Production and Habitat Survey

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Waterfowl Production and Habitat Survey, conducted by the U.S. Fish and Wildlife Service, became operational in 1955 in the Canadian prairies (strata 26-40). In...

  7. Habitat--Offshore Monterey, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for the habitat map of the seafloor of the Offshore of Monterey map area, California. The vector data file is included in...

  8. Riparian Habitat - San Joaquin River

    Data.gov (United States)

    California Department of Resources — The immediate focus of this study is to identify, describe and map the extent and diversity of riparian habitats found along the main stem of the San Joaquin River,...

  9. Habitat--Offshore Monterey, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for the habitat map of the seafloor of the Offshore of Monterey map area, California. The vector data file is included in...

  10. Autonomous Systems: Habitat Automation Element

    Data.gov (United States)

    National Aeronautics and Space Administration — We define a habitat fairly broadly to include any enclosed space that is intended to house people for an extended period of time away from the Earth....

  11. Shrub-Scrub Habitat Evaluation

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Conversion of the current shrub-scrub habitats west of Sandpiper Road and north of the Back BayNational Wildlife Refuge, into recreational facilities for a new hotel...

  12. Predictive value of FSH, testicular volume, and histopathological findings for the sperm retrieval rate of microdissection TESE in nonobstructive azoospermia: a meta-analysis.

    Science.gov (United States)

    Li, Hao; Chen, Li-Ping; Yang, Jun; Li, Ming-Chao; Chen, Rui-Bao; Lan, Ru-Zhu; Wang, Shao-Gang; Liu, Ji-Hong; Wang, Tao

    2017-03-24

    We performed this meta-analysis to evaluate the predictive value of different parameters in the sperm retrieval rate (SRR) of microdissection testicular sperm extraction (TESE) in patients with nonobstructive azoospermia (NOA). All relevant studies were searched in PubMed, Web of Science, EMBASE, Cochrane Library, and EBSCO. We chose three parameters to perform the meta-analysis: follicle-stimulating hormone (FSH), testicular volume, and testicular histopathological findings which included three patterns: hypospermatogenesis (HS), maturation arrest (MA), and Sertoli-cell-only syndrome (SCOS). If there was a threshold effect, only the area under the summary receiver operating characteristic curve (AUSROC) was calculated. Otherwise, the pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and the diagnostic odds ratio (DOR) were also calculated. Twenty-one articles were included in our study finally. There was a threshold effect among studies investigating FSH and SCOS. The AUSROCs of FSH, testicular volume, HS, MA, and SCOS were 0.6119, 0.6389, 0.6758, 0.5535, and 0.2763, respectively. The DORs of testicular volume, HS, and MA were 1.98, 16.49, and 1.26, respectively. The sensitivities of them were 0.80, 0.30, and 0.27, while the specificities of them were 0.35, 0.98, and 0.76, respectively. The PLRs of them were 1.49, 10.63, and 1.15, respectively. And NLRs were 0.73, 0.72, and 0.95, respectively. All the investigated factors in our study had limited predictive value. However, the histopathological findings were helpful to some extent. Most patients with HS could get sperm by microdissection TESE.

  13. Transcription factor p53 can regulate proliferation, apoptosis and secretory activity of luteinizing porcine ovarian granulosa cell cultured with and without ghrelin and FSH.

    Science.gov (United States)

    Sirotkin, A V; Benco, A; Tandlmajerova, A; Vasícek, D; Kotwica, J; Darlak, K; Valenzuela, F

    2008-11-01

    The aim of our in vitro experiments was to examine the role of transcription factor p53 in controlling the basic functions of ovarian cells and their response to hormonal treatments. Porcine ovarian granulosa cells, transfected and non-transfected with a gene construct encoding p53, were cultured with ghrelin and FSH (all at concentrations of 0, 1, 10, or 100 ng/ml). Accumulation of p53, of apoptosis-related (MAP3K5) and proliferation-related (cyclin B1) substances was evaluated by immunocytochemistry. The secretion of progesterone (P(4)), oxytocin (OT), prostaglandin F (PGF), and E (PGE) was measured by RIA. Transfection with the p53 gene construct promoted accumulation of this transcription factor within cells. It also stimulated the expression of a marker of apoptosis (MAP3K5). Over-expression of p53 resulted in reduced accumulation of a marker of proliferation (cyclin B1), P(4), and PGF secretion and increased OT and PGE secretion. Ghrelin, when added alone, did not affect p53 or P(4), but reduced MAP3K5 and increased PGF and PGE secretion. Over-expression of p53 reversed the effect of ghrelin on OT, caused it to be inhibitory to P(4) secretion, but did not modify its action on MAP3K5, PGF, or PGE. FSH promoted the accumulation of p53, MAP3K5, and cyclin B1; these effects were unaffected by p53 transfection. These multiple effects of the p53 gene construct on luteinizing granulosa cells, cultured with and without hormones 1) demonstrate the effects of ghrelin and FSH on porcine ovarian cell apoptosis and secretory activity, 2) confirm the involvement of p53 in promoting apoptosis and inhibiting P(4) secretion in these cells, 3) provide the first evidence that p53 suppress proliferation of ovarian cells, 4) provide the first evidence that p53 is involved in the control of ovarian peptide hormone (OT) and prostaglandin (PGF and PGE) secretion, and 5) suggest that p53 can modulate, but probably not mediate, the effects of ghrelin and FSH on the ovary.

  14. Geopressured habitat: A literature review

    Energy Technology Data Exchange (ETDEWEB)

    Negus-de Wys, Jane

    1992-09-01

    A literature review of the geopressured-geothermal habitat is summarized. Findings are presented and discussed with respect to the principal topics: Casual agents are both geological and geochemical; they include disequilibrium compaction of sediments, clay diagenesis, aquathermal pressuring, hydrocarbon generation, and lateral tectonic compression. The overall physical and chemical characteristics of the habitats are dictated by varying combinations of sedimentation rates, alteration mineralogy, permeability, porosity and pressure, temperature, fluid content and chemistry, and hydrodynamic flow. Habitat pressure seals are considered in terms of their formation processes, geologic characteristics, and physical behavior, including pressure release and reservoir pressure recharge on a geologic time scale. World-wide occurrence of geopressured-geothermal habitats is noted. The main thrust of this topic concerns the U.S.A. and Canada; in addition, reference is made to occurrences in China and indications from deep-sea vents, as well as the contribution of paleo-overpressure to habitat initiation and maintenance. Identification and assessment of the habitat is addressed in relation to use of hydrogeologic, geophysical, geochemical, and geothermic techniques, as well as well-logging and drill-stem-test data. Conclusions concerning the adequacy of the current state of knowledge and its applicability to resource exploration and development are set forth, together with recommendations for the thrust of future work.

  15. Dormaier and Chester Butte 2007 Follow-up Habitat Evaluation Procedures Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-01-01

    Follow-up habitat evaluation procedures (HEP) analyses were conducted on the Dormaier and Chester Butte wildlife mitigation sites in April 2007 to determine the number of additional habitat units to credit Bonneville Power Administration (BPA) for providing funds to enhance, and maintain the project sites as partial mitigation for habitat losses associated with construction of Grand Coulee Dam. The Dormaier follow-up HEP survey generated 482.92 habitat units (HU) or 1.51 HUs per acre for an increase of 34.92 HUs over baseline credits. Likewise, 2,949.06 HUs (1.45 HUs/acre) were generated from the Chester Butte follow-up HEP analysis for an increase of 1,511.29 habitat units above baseline survey results. Combined, BPA will be credited with an additional 1,546.21 follow-up habitat units from the Dormaier and Chester Butte parcels.

  16. Natural Propagation and Habitat Improvement, Volume 2, Idaho, 1985 Annual and Final Reports.

    Energy Technology Data Exchange (ETDEWEB)

    Hair, Don

    1986-09-01

    The individual reports in this volume have been separately abstracted for inclusion in the data base. The reports describe fish habitat enhancement projects on the Lochsa River, Eldorado and Camas Creeks, and the Clearwater River. (ACR)

  17. Natural Propagation and Habitat Improvement, Volume I, Oregon, 1984 Final and Annual Reports.

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Rod

    1986-02-01

    This volume contains reports on habitat improvement and fisheries enhancement projects conducted in the following subbasins: (1) Clackamas River; (2) Hood River; :(3) Deschutes River; (4) John Day River; (5) Umatilla River; and (6) Grande Ronde River. (ACR)

  18. Variability and convergence in benthic communities in created salt marshes transitioning into mangrove habitats

    Science.gov (United States)

    Wetland creation, enhancement, and restoration activities are commonly implemented to compensate for wetland loss or degradation in coastal ecosystems. Although assessments of structural condition are commonly used to monitor habitat restoration effectiveness, functional equivale...

  19. Grizzly Bear Aware: Conflict Resolution and Habitat Restoration in the Centennial Valley and Southwest Montana

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The project will take a three pronged approach to implement conservation actions that prevent or reduce Grizzly Bear human conflicts, enhance habitats and improve...

  20. West Foster Creek 2007 Follow-up Habitat Evaluation Procedures (HEP) Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-02-01

    A follow-up habitat evaluation procedures (HEP) analysis was conducted on the West Foster Creek (Smith acquisition) wildlife mitigation site in May 2007 to determine the number of additional habitat units to credit Bonneville Power Administration (BPA) for providing funds to enhance and maintain the project site as partial mitigation for habitat losses associated with construction of Grand Coulee Dam. The West Foster Creek 2007 follow-up HEP survey generated 2,981.96 habitat units (HU) or 1.51 HUs per acre for a 34% increase (+751.34 HUs) above baseline HU credit (the 1999 baseline HEP survey generated 2,230.62 habitat units or 1.13 HUs per acre). The 2007 follow-up HEP analysis yielded 1,380.26 sharp-tailed grouse (Tympanuchus phasianellus) habitat units, 879.40 mule deer (Odocoileus hemionus) HUs, and 722.29 western meadowlark (Sturnella neglecta) habitat units. Mule deer and sharp-tailed grouse habitat units increased by 346.42 HUs and 470.62 HUs respectively over baseline (1999) survey results due largely to cessation of livestock grazing and subsequent passive restoration. In contrast, the western meadowlark generated slightly fewer habitat units in 2007 (-67.31) than in 1999, because of increased shrub cover, which lowers habitat suitability for that species.

  1. A NEW HABITAT CLASSIFICATION AND MANUAL FOR STANDARDIZED HABITAT MAPPING

    Directory of Open Access Journals (Sweden)

    J. BOLONI

    2007-04-01

    Full Text Available Today the documentation of natural heritage with scientific methods but for conservation practice – like mapping of actual vegetation – becomes more and more important. For this purpose mapping guides containing only the names and descriptions of vegetation types are not sufficient. Instead, new, mapping-oriented vegetation classification systems and handbooks are needed. There are different standardised systems fitted to the characteristics of a region already published and used successfully for surveying large territories. However, detailed documentation of the aims and steps of their elaboration is still missing. Here we present a habitat-classification method developed specifically for mapping and the steps of its development. Habitat categories and descriptions reflect site conditions, physiognomy and species composition as well. However, for species composition much lower role was given deliberately than in the phytosociological systems. Recognition and mapping of vegetation types in the field is highly supported by a definition, list of subtypes and list of ‘types not belonging to this habitat category’. Our system is two-dimensional: the first dimension is habitat type, the other is naturalness based habitat quality. The development of the system was conducted in two steps, over 200 mappers already tested it over 7000 field days in different projects.

  2. A NEW HABITAT CLASSIFICATION AND MANUAL FOR STANDARDIZED HABITAT MAPPING

    Directory of Open Access Journals (Sweden)

    A. KUN

    2007-01-01

    Full Text Available Today the documentation of natural heritage with scientific methods but for conservation practice – like mapping of actual vegetation – becomes more and more important. For this purpose mapping guides containing only the names and descriptions of vegetation types are not sufficient. Instead, new, mapping-oriented vegetation classification systems and handbooks are needed. There are different standardised systems fitted to the characteristics of a region already published and used successfully for surveying large territories. However, detailed documentation of the aims and steps of their elaboration is still missing. Here we present a habitat-classification method developed specifically for mapping and the steps of its development. Habitat categories and descriptions reflect site conditions, physiognomy and species composition as well. However, for species composition much lower role was given deliberately than in the phytosociological systems. Recognition and mapping of vegetation types in the field is highly supported by a definition, list of subtypes and list of ‘types not belonging to this habitat category’. Our system is two-dimensional: the first dimension is habitat type, the other is naturalness based habitat quality. The development of the system was conducted in two steps, over 200 mappers already tested it over 7000 field days in different projects.

  3. Habitat Evaluation Procedures (HEP) Report; Priest River Project, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    On July 6, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Priest River property, an acquisition completed by the Kalispel Tribe of Indians in 2001. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Priest River Project provides a total of 140.73 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 60.05 HUs for bald eagle, black-capped chickadee, and white-tailed deer. Grassland meadow habitat provides 7.39 HUs for Canada goose and mallard. Scrub-shrub vegetation provides 71.13 HUs for mallard, yellow warbler, and white-tailed deer. Open water habitat provides 2.16 HUs for Canada goose and mallard. The objective of using HEP at the Priest River Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  4. Habitat Evaluation Procedures (HEP) Report; North Eaton Lake, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-11-01

    On July 6, 2005, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the North Eaton Lake property, an acquisition completed by the Kalispel Tribe of Indians in November 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The North Eaton Lake Project provides a total of 235.05 Habitat Units (HUs) for the species evaluated. Open water habitat provides 9.38 HUs for Canada goose, mallard and muskrat. Emergent wetland habitat provides 11.36 HUs for Canada goose, mallard and muskrat. Forested wetland provides 10.97 HUs for bald eagle, black-capped chickadee, mallard and white-tailed deer. Conifer forest habitat provides 203.34 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. The objective of using HEP at the North Eaton Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  5. Expression of 17?-hydroxysteroid dehydrogenase and the effects of LH, FSH and prolactin on oestrone and 17?-oestradiol secretion in the endometrium of pigs during early pregnancy and the oestrous cycle.

    Science.gov (United States)

    Wojciechowicz, B; Kotwica, G; Zglejc, K; Waszkiewicz, E; Franczak, A

    2016-03-07

    The endometrium of pregnant and cyclic pigs is a source of oestrone (E1) and 17β-oestradiol (E2). However, the roles of LH, FSH and prolactin (PRL) as regulators of endometrial steroidogenesis, and the presence of 17β-hydroxysteroid dehydrogenase (17β-HSD) in the porcine endometrium, remain unknown. Therefore, in the present study we examined 17β-HSD expression and the effects of LH, FSH and PRL on E1 and E2 release in vitro in endometrial explants harvested from gravid pigs on Days 10-11 (embryo migration within the uterus), 12-13 (maternal recognition of pregnancy) and 15-16 (beginning of implantation) and compared them with results obtained in non-gravid pigs. The results show that: (1) endometrial 17β-HSD activity was decreased on Days 15-16 in pregnant and cyclic pigs compared with the preceding days; (2) LH, FSH and PRL increased endometrial E1 secretion on Days 10-11 and 15-16 of pregnancy and on Days 12-13 and 15-16 of the oestrous cycle; and (3) LH, FSH and PRL increased endometrial E2 secretion on Days 15-16 of pregnancy and during the days studied in the oestrous cycle. In conclusion, data suggest that LH, FSH and PRL affect endometrial secretion of estrogens in pigs.

  6. Habitat Suitability Index Models: Eastern wild turkey

    Science.gov (United States)

    Schroeder, Richard L.

    1985-01-01

    A review and synthesis of existing information were used to develop a Habitat Suitability Index (HSI) model for the eastern wild turkey (Meleagris gallopavo sylvestris). The model consolidates habitat use information into a framework appropriate for field application, and is scaled to produce an index between 0.0 (unsuitable habitat) to 1.0 (optimum habitat). HSI models are designed to be used with Habitat Evaluation Procedures previously developed by the U.S. Fish and Wildlife Service.

  7. Habitat Suitability Index Models: Swamp rabbit

    Science.gov (United States)

    Allen, Arthur W.

    1985-01-01

    A review and synthesis of existing information were used to develop a Habitat Suitability Index (HSI) model for the swamp rabbit (Sylvilagus aquaticus). The model consolidates habitat use information into a framework appropriate for field application, and is scaled to produce an index between 0.0 (unsuitable habitat) to 1.0 (optimum habitat). HSI models are designed to be used with Habitat Evaluation Procedures previously developed by the U.S. Fish and Wildlife Service.

  8. Species persistence in landscapes with spatial variation in habitat quality: a pair approximation model.

    Science.gov (United States)

    Liao, Jinbao; Li, Zhenqing; Hiebeler, David E; Iwasa, Yoh; Bogaert, Jan; Nijs, Ivan

    2013-10-21

    Habitat degradation has become a major threat to species persistence. Although several models have explicitly integrated habitat quality into metapopulation dynamics, we still lack knowledge of the spatial variability of species persistence which may result from the clustering of habitat patches of differing quality. Here we construct both pair approximation (PA) and cellular automaton (CA) models for species persistence in homogeneous versus heterogeneous landscapes. Heterogeneous landscapes are generated by varying the orthogonal-neighbour correlation between two different-quality habitats. In our simulations, the PA model exhibits similar population dynamics to the CA model, though it overestimates species persistence due to the doublet approximation neglecting correlation beyond nearest neighbours. Generally, landscape heterogeneity enhances species persistence relative to landscape homogeneity, especially with enlarging habitat-quality difference. This indicates that models based on homogeneous landscapes may overestimate species extinction rate. In heterogeneous landscapes, habitat clumping does not influence global dispersers because of random establishment, although it does promote the persistence of local dispersers, especially under severe habitat degradation. However, habitat configurational fragmentation improves the persistence of global dispersers that are highly sensitive to local crowding, probably by reducing density dependence, but this positive fragmentation effect on local dispersers is overshadowed by the stronger negative border effect on impeding local extension. Furthermore, increasing density dependence promotes the extinction risk of local dispersers, while global dispersers are not influenced. For conservation and habitat management, our results suggest that minimising random anthropogenic disturbance should take priority over increasing the connectivity of good-quality habitat, as random habitat degradation poses a more serious threat to

  9. From Satellite Imagery to Peatland Vegetation Diversity: How Reliable Are Habitat Maps?

    Directory of Open Access Journals (Sweden)

    Monique F. Poulin

    2002-12-01

    Full Text Available Although satellite imagery is becoming a basic component of the work of ecologists and conservationists, its potential and reliability are still relatively unknown for a large number of ecosystems. Using Landsat 7/ETM+ (Enhanced Thematic Mapper Plus data, we tested the accuracy of two types of supervised classifications for mapping 13 peatland habitats in southern Quebec, Canada. Before classifying peatland habitats, we applied a mask procedure that revealed 629 peatlands covering a total of 18,103 ha; 26% of them were larger than 20 ha. We applied both a simple maximum likelihood (ML function and a weighted maximum likelihood (WML function that took into account the proportion of each habitat class within each peatland when classifying the habitats on the image. By validating 626 Global Positioning System locations within 92 peatlands, we showed that both classification procedures provided an accurate representation of the 13 peatland habitat classes. For all habitat classes except lawn with pools, the predominant classified habitat within 45 m of the center of the validation location was of the same type as the one observed in the field. There were differences in the performance of the two classification procedures: ML was a better tool for mapping rare habitats, whereas WML favored the most common habitats. Based on ordinations, peatland habitat classes were as effective as environmental variables such as humidity indicators and water chemistry components at explaining the distribution of plant species and performed 1.6 times better when it came to accounting for vegetation structure patterns. Peatland habitats with pools had the most distinct plant assemblages, and the habitats dominated by herbs were moderately distinct from those characterized by ericaceous shrubs. Habitats dominated by herbs were the most variable in terms of plant species assemblages. Because peatlands are economically valuable wetlands, the maps resulting from the new

  10. Determining habitat quality for species that demonstrate dynamic habitat selection

    Science.gov (United States)

    Beerens, James; Frederick, Peter C; Noonburg, Erik G; Gawlik, Dale E.

    2015-01-01

    Determining habitat quality for wildlife populations requires relating a species' habitat to its survival and reproduction. Within a season, species occurrence and density can be disconnected from measures of habitat quality when resources are highly seasonal, unpredictable over time, and patchy. Here we establish an explicit link among dynamic selection of changing resources, spatio-temporal species distributions, and fitness for predictive abundance and occurrence models that are used for short-term water management and long-term restoration planning. We used the wading bird distribution and evaluation models (WADEM) that estimate (1) daily changes in selection across resource gradients, (2) landscape abundance of flocks and individuals, (3) conspecific foraging aggregation, and (4) resource unit occurrence (at fixed 400 m cells) to quantify habitat quality and its consequences on reproduction for wetland indicator species. We linked maximum annual numbers of nests detected across the study area and nesting success of Great Egrets (Ardea alba), White Ibises (Eudocimus albus), and Wood Storks (Mycteria americana) over a 20-year period to estimated daily dynamics of food resources produced by WADEM over a 7490 km2 area. For all species, increases in predicted species abundance in March and high abundance in April were strongly linked to breeding responses. Great Egret nesting effort and success were higher when birds also showed greater conspecific foraging aggregation. Synthesis and applications: This study provides the first empirical evidence that dynamic habitat selection processes and distributions of wading birds over environmental gradients are linked with reproductive measures over periods of decades. Further, predictor variables at a variety of temporal (daily-multiannual) resolutions and spatial (400 m to regional) scales effectively explained variation in ecological processes that change habitat quality. The process used here allows managers to develop

  11. Avaliação dos efeitos do estradiol e do FSH nos níveis de leptina em mulheres com supressão da função hipofisária Effects of estradiol and FSH on leptin levels in women with pituitary suppression

    Directory of Open Access Journals (Sweden)

    Selmo Geber

    2005-04-01

    Full Text Available OBJETIVO: identificar a correlação entre os níveis séricos de leptina e os níveis de estradiol e do hormônio folículo-estimulante (FSH em mulheres com supressão da função hipofisária, e suas possíveis interferências no eixo reprodutivo. MÉTODOS: estudamos prospectivamente 64 pacientes submetidas à hiperestimulação ovariana controlada com FSH recombinante para tratamento pela técnica de reprodução assistida, devido a fator masculino ou tubário, e 20 pacientes em uso de valerato de estradiol, para preparo endometrial, em tratamento de doação de óvulos, por falha de resposta ovariana em ciclo prévio. Todas as pacientes utilizaram análogo de GnRH no início do tratamento, de forma a obter a supressão da função hipofisária. Para a análise estatística dos resultados, foram utilizados os testes chi2, t de Student e correlação de Pearson, quando adequado. Os resultados foram considerados significativos quando pPURPOSE: to identify the relationship between serum levels of leptin and the levels of estradiol and follicle-stimulating hormone (FSH in women with pituitary suppression and to evaluate its possible interference on the reproductive axis. METHODS: a total of 64 patients submitted to controlled ovarian hyperstimulation with recombinant FSH for assisted reproduction, due to a male or tubal factor, and 20 patients using estradiol valerate, for endometrial preparation in order to be submitted to oocyte donation treatment were studied. All patients used GnRH analogues before starting treatment in order to avoid premature LH surge. Data were analyzed statistically by the chi2 test, Student's t-test and the Pearson correlation test, when appropriate, with the level of significance set at p<0,05. RESULTS: it was observed that leptin levels correlated with body mass index (BMI even though they had not influenced growth rate of these hormones. A positive correlation was observed between estradiol and leptin levels in both

  12. Wetland habitats for wildlife of the Chesapeake Bay

    Science.gov (United States)

    Perry, M.C.; Majumdar, S.K.; Miller, E.W.; Brenner, Fred J.

    1998-01-01

    The wetlands of Chesapeake Bay have provided the vital habitats that have sustained the impressive wildlife populations that have brought international fame to the Bay. As these wetland habitats decrease in quantity and quality we will continue to see the decline in the wildlife populations that started when European settlers first came to this continent. These declines have accelerated significantly in this century. As the human population continues to increase in the Bay watershed, one can expect that wetland habitats will continue to decline, resulting in declines in species diversity and population numbers. Although federal, state, and local governments are striving for 'no net loss' of wetlands, the results to date are not encouraging. It is unrealistic to believe that human populations and associated development can continue to increase and not adversely affect the wetland resources of the Bay. Restrictions on human population growth in the Chesapeake area is clearly the best way to protect wetland habitats and the wildlife that are dependent on these habitats. In addition, there should be more aggressive approaches to protect wetland habitats from continued perturbations from humans. More sanctuary areas should be created and there should be greater use of enhancement and management techniques that will benefit the full complement of species that potentially exist in these wetlands. The present trend in wetland loss can be expected to continue as human populations increase with resultant increases in roads, shopping malls, and housing developments. Creation of habitat for mitigation of these losses will not result in 'no net loss'. More innovative approaches should be employed to reverse the long-term trend in wetland loss by humans.

  13. Trapping Triatominae in Silvatic Habitats

    Directory of Open Access Journals (Sweden)

    Noireau François

    2002-01-01

    Full Text Available Large-scale trials of a trapping system designed to collect silvatic Triatominae are reported. Live-baited adhesive traps were tested in various ecosystems and different triatomine habitats (arboreal and terrestrial. The trials were always successful, with a rate of positive habitats generally over 20% and reaching 48.4% for palm trees of the Amazon basin. Eleven species of Triatominae belonging to the three genera of public health importance (Triatoma, Rhodnius and Panstrongylus were captured. This trapping system provides an effective way to detect the presence of triatomines in terrestrial and arboreal silvatic habitats and represents a promising tool for ecological studies. Various lines of research are contemplated to improve the performance of this trapping system.

  14. Loss and modification of habitat

    Science.gov (United States)

    Lemckert, Francis; Hecnar, Stephen; Pilliod, David S.; Wilkinson, John W.; Heatwole, Harold

    2012-01-01

    Amphibians live in a wide variety of habitats around the world, many of which have been modified or destroyed by human activities. Most species have unique life history characteristics adapted to specific climates, habitats (e.g., lentic, lotic, terrestrial, arboreal, fossorial, amphibious), and local conditions that provide suitable areas for reproduction, development and growth, shelter from environmental extremes, and predation, as well as connectivity to other populations or habitats. Although some species are entirely aquatic or terrestrial, most amphibians, as their name implies, lead a dual life and require a mosaic of habitats in both aquatic and terrestrial ecosystems. With over 6 billion people on Earth, most species are now persisting in habitats that have been directly or indirectly influenced by human activities. Some species have disappeared where their habitats have been completely destroyed, reduced, or rendered unsuitable. Habitat loss and degradation are widely considered by most researchers as the most important causes of amphibian population decline globally (Barinaga 1990; Wake and Morowitz 1991; Alford and Richards 1999). In this chapter, a background on the diverse habitat requirements of amphibians is provided, followed by a discussion of the effects of urbanization, agriculture, livestock grazing, timber production and harvesting, fire and hazardous fuel management, and roads on amphibians and their habitats. Also briefly discussed is the influence on amphibian habitats of natural disturbances, such as extreme weather events and climate change, given the potential for human activities to impact climate in the longer term. For amphibians in general, microhabitats are of greater importance than for other vertebrates. As ectotherms with a skin that is permeable to water and with naked gelatinous eggs, amphibians are physiologically constrained to be active during environmental conditions that provide appropriate body temperatures and adequate

  15. Habitat Suitability Index Models: Mink

    Science.gov (United States)

    Allen, Arthur W.

    1983-01-01

    The mink (Mustela vison) is a predatory, semiaquatic mammal that is generally associated with stream and river banks, lake shores, fresh and saltwater marshes, and marine shore habitats (Gerell 1970).  Mink are chiefly nocturnal and remain active throughout the year (Marshall 1936); Gerell 1969; Burgess 1978).  The species is adaptable in its use of habitat, modifying daily habits according to environmental conditions, particularly prey availability (Wise et al. 1981; Linn and Birds 1981; Birks and Linn 1982).  The species is tolerant of human activity and will inhabit suboptimum habitats as long as an adequate food source is available; however, mink will be more mobile and change home ranges more frequently under such conditions (Linn pers. comm.).

  16. CTUIR Umatilla Anadromous Fisheries Habitat Project : A Columbia River Basin Fish Habitat Project 2008 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Hoverson, Eric D.; Amonette, Alexandra

    2009-02-09

    The Umatilla Anadromous Fisheries Habitat Project (UAFHP) is an ongoing effort to protect, enhance, and restore riparian and instream habitat for the natural production of anadromous salmonids in the Umatilla River Basin, Northeast Oregon. Flow quantity, water temperature, passage, and lack of in-stream channel complexity have been identified as the key limiting factors in the basin. During the 2008 Fiscal Year (FY) reporting period (February 1, 2008-January 31, 2009) primary project activities focused on improving instream and riparian habitat complexity, migrational passage, and restoring natural channel morphology and floodplain function. Eight primary fisheries habitat enhancement projects were implemented on Meacham Creek, Birch Creek, West Birch Creek, McKay Creek, West Fork Spring Hollow, and the Umatilla River. Specific restoration actions included: (1) rectifying one fish passage barrier on West Birch Creek; (2) participating in six projects planting 10,000 trees and seeding 3225 pounds of native grasses; (3) donating 1000 ft of fencing and 1208 fence posts and associated hardware for 3.6 miles of livestock exclusion fencing projects in riparian areas of West Birch and Meacham Creek, and for tree screens to protect against beaver damage on West Fork Spring Hollow Creek; (4) using biological control (insects) to reduce noxious weeds on three treatment areas covering five acres on Meacham Creek; (5) planning activities for a levee setback project on Meacham Creek. We participated in additional secondary projects as opportunities arose. Baseline and ongoing monitoring and evaluation activities were also completed on major project areas such as conducting photo point monitoring strategies activities at the Meacham Creek Large Wood Implementation Project site (FY2006) and at additional easements and planned project sites. Fish surveys and aquatic habitat inventories were conducted at project sites prior to implementation. Proper selection and implementation of

  17. Rhizomatic, digital habitat

    DEFF Research Database (Denmark)

    Kjærgaard, Thomas; Sorensen, Elsebeth Korsgaard

    2014-01-01

    and knowledge building. The paper suggests a new way/model of describing key competences for learning in the future based on ‘network literacy’. The competence to produce knowledge through information fragments in a hierarchical, rhizomatic network seems to be a vital competence when learners produce knowledge...... on the basis of internet information. Also, a teacher’s network literacy and his/her competency to utilize different, connected technologies is necessary when learners act and learn within a rhizomatic network. The authors claim that when the classroom becomes a ‘digital habitat’ and the learning processes...... are social in a connected rhizomatic network then information sharing, knowledge production and technology embody a strong synergetic entity. The study suggests that technologies used to create ’digital habitats’ are likely to enhance learning and motivation and allow new creative ways of working...

  18. Private lands habitat programs benefit California's native birds

    Directory of Open Access Journals (Sweden)

    Ryan T. DiGaudio

    2015-10-01

    Full Text Available To address the loss of wetlands and riparian forests in California, private lands habitat programs are available through U.S. federal and state government agencies to help growers, ranchers and other private landowners create and enhance wildlife habitat. The programs provide financial and technical assistance for implementing conservation practices. To evaluate the benefits of these programs for wildlife, we examined bird use of private wetlands, postharvest flooded croplands and riparian forests enrolled in habitat programs in the Central Valley and North Coast regions of California. We found that private Central Valley wetlands supported 181 bird species during the breeding season. During fall migration, postharvest flooded croplands supported wetland-dependent species and a higher density of shorebirds than did semipermanent wetlands. At the riparian sites, bird species richness increased after restoration. These results demonstrated that the programs provided habitat for the species they were designed to protect; a variety of resident and migratory bird species used the habitats, and many special status species were recorded at the sites.

  19. Instream Physical Habitat Modelling Types

    DEFF Research Database (Denmark)

    Conallin, John; Boegh, Eva; Krogsgaard, Jørgen

    2010-01-01

    management tools, but require large amounts of data and the model structure is complex. It is concluded that the use of habitat suitability indices (HSIs) and fuzzy rules in hydraulic-habitat modelling are the most ready model types to satisfy WFD demands. These models are well documented, transferable, user-friendly...... and disadvantages as management tools for member states in relation to the requirements of the WFD, but due to their different model structures they are distinct in their data needs, transferability, user-friendliness and presentable outputs. Water resource managers need information on what approaches will best...

  20. Camas Creek (Meyers Cove) Anadromous Species Habitat Improvement: Annual Report 1989.

    Energy Technology Data Exchange (ETDEWEB)

    Hardy, Terry

    1989-12-01

    Historical agricultural practices and natural events contributed to severe degradation of riparian zones and instream fish habitat in the Meyers Cove area of Camas Creek. In 1984, Salmon National Forest personnel began implementing specific management activities in riparian areas and the stream channel to accelerate habitat recovery. In 1987--88, 4.3 miles of fence was constructed establishing a riparian livestock exclosure in the Meyers Cove area of Camas Creek. One end-gap and two water-crossing corridors were constructed in 1989 to complete the fence system. The riparian exclosure has been fertilized with phosphorous-rich fertilizer to promote root growth. A stream crossing ford was stabilized with angular cobble. Streambank stabilization/habitat cover work was completed at three sites and three additional habitat structures were placed. Extensive habitat inventories were completed to identify quality/quantity of habitat available to anadromous fish. The work accomplished was designed to promote natural revegetation of the riparian area to improve rearing habitat cover and streambank stability. Streambank work was limited to extremely unstable sites. Enhancement activities will improve spawning, incubation, and rearing habitat for wild populations of steelhead trout and chinook salmon. Anadromous species population increases resulting from these enhancement activities will provide partial compensation for downstream losses resulting from hydroelectric developments on the Columbia River system. 9 refs., 6 figs., 7 tabs.

  1. Habitat Evaluation Procedures (HEP) Report; West Beaver Lake Project, Technical Report 2005

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    On September 7, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the West Beaver Lake property, an acquisition completed by the Kalispel Tribe of Indians in September 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, mallard, muskrat, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The West Beaver Lake Project provides a total of 82.69 Habitat Units (HUs) for the species evaluated. Emergent wetland habitat provides 8.80 HUs for mallard, muskrat, and Canada goose. Conifer forest habitat provides 70.33 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Open water provides 3.30 HUs for mallard, muskrat, and Canada goose. The objective of using HEP at the West Beaver Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  2. Habitat Evaluation Procedures (HEP) Report, Pend Oreille Wetlands Wildlife II Project, Technical Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Holmes, Darren

    2003-06-01

    In 2002, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Pend Oreille Wetlands Wildlife II Project, an acquisition completed by the Kalispel Tribe of Indians in 1997. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, and yellow warbler. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Pend Oreille Wetlands Wildlife II Project provides a total of 313.91 Habitat Units (HUs) for the species evaluated. Open water habitat provides 16.08 HUs for Canada goose and mallard. Shoreline and island habitat provide 7.36 HUs fore Canada goose and mallard. Wet meadow provides 117.62 HUs for Canada goose and mallard. Scrub-shrub wetlands provide 9.78 HUs for yellow warbler, mallard, and white-tailed deer. Deciduous forested wetlands provide 140.47 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Conifer forest provides 22.60 HUs for bald eagle, black-capped chickadee, and white-tailed deer. The objective of using HEP at the Pend Oreille Wetlands Wildlife II Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  3. A Comparative Study of Fish Assemblages Near Aquaculture, Artificial and Natural Habitats

    Institute of Scientific and Technical Information of China (English)

    WANG Zhenhua; CHEN Yong; ZHANG Shouyu; WANG Kai; ZHAO Jing; XU Qiang

    2015-01-01

    Habitat plays a critical role in regulating fish community structure. Using the data collected from a monthly trammel net survey in Ma’an archipelago off the east coast of China, we evaluated impacts of five different habitats (artificial reefs, mussel farms, cage aquaculture, rocky reefs and soft bottom) on fish assemblages. This study suggests that artificial reefs (AR) have significantly higher species richness, abundance and diversity than mussel farms (MF) or soft bottom (SB) habitats during most seasons, and that fish taxa in the AR habitats are similar to those in the rocky reef (RR) habitats. Two different fish assemblage patterns were revealed in the study area using non-metric multidimensional scaling ordination: an assemblage dominated by reef fishes (especially by Scor-paenidae species) in AR, RR and cage aquaculture (CA) habitats and an assemblage dominated by Sciaenidae species in MF and SB habitats. We suggest that reef fishes play a key role in differentiating fish community structures in the study area. Although few dif-ferences in fish abundance and diversity were found between the CA and SB habitats, a more diverse age structure was observed in the CA habitats. A much more complex fish assemblage and enhanced population of local species were established as a result of the presence of both floating and fixed artificial structures, probably through improved survival rates.

  4. A comparative study of fish assemblages near aquaculture, artificial and natural habitats

    Science.gov (United States)

    Wang, Zhenhua; Chen, Yong; Zhang, Shouyu; Wang, Kai; Zhao, Jing; Xu, Qiang

    2015-02-01

    Habitat plays a critical role in regulating fish community structure. Using the data collected from a monthly trammel net survey in Ma'an archipelago off the east coast of China, we evaluated impacts of five different habitats (artificial reefs, mussel farms, cage aquaculture, rocky reefs and soft bottom) on fish assemblages. This study suggests that artificial reefs (AR) have significantly higher species richness, abundance and diversity than mussel farms (MF) or soft bottom (SB) habitats during most seasons, and that fish taxa in the AR habitats are similar to those in the rocky reef (RR) habitats. Two different fish assemblage patterns were revealed in the study area using non-metric multidimensional scaling ordination: an assemblage dominated by reef fishes (especially by Scorpaenidae species) in AR, RR and cage aquaculture (CA) habitats and an assemblage dominated by Sciaenidae species in MF and SB habitats. We suggest that reef fishes play a key role in differentiating fish community structures in the study area. Although few differences in fish abundance and diversity were found between the CA and SB habitats, a more diverse age structure was observed in the CA habitats. A much more complex fish assemblage and enhanced population of local species were established as a result of the presence of both floating and fixed artificial structures, probably through improved survival rates.

  5. Seasonal variation in habitat use of juvenile Steelhead in a tributary of Lake Ontario

    Science.gov (United States)

    Studdert, Emily W.; Johnson, James H.

    2015-01-01

    We examined seasonal-habitat use by subyearling and yearling Oncorhynchus mykiss (Rainbow Trout or Steelhead) in Trout Brook, a tributary of the Salmon River, NY. We determined daytime fish-habitat use and available habitat during August and October of the same year and observed differences in habitat selection among year classes. Water depth and cover played the greatest role in Steelhead habitat use. During summer and autumn, we found yearling Steelhead in areas with deeper water and more cover than where we observed subyearling Steelhead. Both year classes sought out areas with abundant cover during both seasons; this habitat was limited within the stream reach. Subyearling Steelhead were associated with more cover during autumn, even though available cover within the stream reach was greater during summer. Principal component analysis showed that variation in seasonal-habitat use was most pronounced for subyearling Steelhead and that yearling Steelhead were more selective in their habitat use than subyearling Steelhead. The results of this study contribute to a greater understanding of how this popular sportfish is adapting to a new environment and the factors that may limit juvenile Steelhead survival. Our findings provide valuable new insights into the seasonal-habitat requirements of subyearling and yearling Steelhead that can be used by fisheries managers to enhance and protect the species throughout the Great Lakes region.

  6. Nursery function of coastal temperate benthic habitats: New insight from the bivalve recruitment perspective

    Science.gov (United States)

    Barbier, Pierrick; Meziane, Tarik; Forêt, Martin; Tremblay, Réjean; Robert, René; Olivier, Frédéric

    2017-03-01

    Marine habitat function has been typically investigated in terms of biogeochemical regulation but rarely in terms of population renewal, which is mainly controlled by recruitment dynamics. The recruitment phase is crucial for organisms with a bentho-pelagic life cycle, such as bivalves, and it regulates the population renewal success. This study provides new insight on the role of temperate benthic habitats on bivalve recruitment, as a function of nursery areas. Six dominant benthic habitats of the Chausey archipelago (Normandy, France) were studied. In each habitat, bivalve recruit assemblages were described at the end of two reproductive seasons. Furthermore, Ostrea edulis juveniles were immerged on each habitat during two months to compare growth performances and feeding status, estimated by fatty acid composition. Recruit assemblages differ from each habitat according to sediment grain-size composition and bathymetrical levels. Subtidal habitats, and especially Crepidula fornicata banks and Glycymeris glycymeris coarse sands, supported the highest species abundance and richness of recruits. All O. edulis juveniles fed on the same trophic resources but digestive glands of juveniles from C. fornicata banks were more concentrated in total fatty acids than those from subtidal G. glycymeris coarse sands and maerl banks. Our results depict the key role of subtidal and structured habitats, composed of ecosystem engineers, in enhancing bivalve recruitment and extending the bivalve population renewal. This study suggests that the crucial role of these habitats as bivalve nurseries must be integrated in management perspectives.

  7. Habitat Suitability Index Models: Red king crab

    Science.gov (United States)

    Jewett, Stephen C.; Onuf, Christopher P.

    1988-01-01

    A review and synthesis of existing information were used to develop a Habitat Suitability Index (HSI) model for evaluating habitat of different life stages of red king crab (Paralithodes camtschatica). A model consolidates habitat use information into a framework appropriate for field application, and is scaled to produce an index between 0.0 (unsuitable habitat) and 1.0 (optimum habitat) in Alaskan coastal waters, especially in the Gulf of Alaska and the southeastern Bering Sea. HSI models are designed to be used with Habitat Evaluation Procedures previously developed by the U.S. Fish and Wildlife Service.

  8. Ecological Responses to Trout Habitat Rehabilitation in a Northern Michigan Stream

    Science.gov (United States)

    Rosi-Marshall, Emma J.; Moerke, Ashley H.; Lamberti, Gary A.

    2006-07-01

    Monitoring of stream restoration projects is often limited and success often focuses on a single taxon (e.g., salmonids), even though other aspects of stream structure and function may also respond to restoration activities. The Ottawa National Forest (ONF), Michigan, conducted a site-specific trout habitat improvement to enhance the trout fishery in Cook’s Run, a 3rd-order stream that the ONF determined was negatively affected by past logging. Our objectives were to determine if the habitat improvement increased trout abundances and enhanced other ecological variables (overall habitat quality, organic matter retention, seston concentration, periphyton abundance, sediment organic matter content, and macroinvertebrate abundance and diversity) following rehabilitation. The addition of skybooms (underbank cover structures) and k-dams (pool-creating structures) increased the relative abundance of harvestable trout (>25 cm in total length) as intended but not overall trout abundances. Both rehabilitation techniques also increased maximum channel depth and organic matter retention, but only k-dams increased overall habitat quality. Neither approach significantly affected other ecological variables. The modest ecological response to this habitat improvement likely occurred because the system was not severely degraded beforehand, and thus small, local changes in habitat did not measurably affect most physical and ecological variables measured. However, increases in habitat volume and in organic matter retention may enhance stream biota in the long term.

  9. Seagrasses - The forgotton marine habitat

    Digital Repository Service at National Institute of Oceanography (India)

    Jagtap, T.G.; Rodrigues, R.S.

    constitute the seagrass flora of Goa, and their patches occur in the lower intertidal and shallow littoral, polyhaline (18-30 ppt) zones. The prominent beds in Goa exist along Mandovi and Terekhol estuaries, and Chapora Bay. These habitats in the country have...

  10. Habitats: staging life and art

    DEFF Research Database (Denmark)

    Andersen, Peter Bøgh

    2004-01-01

    -relevantinformation. The habitat concept was originally proposed as a help to understandmobile context-sensitive technology, but turns out to have much broader applications. Thepresent version of the approach uses activity theory and semiotics as the basic theoretical un-derpinnings.The paper offers a notation for specifying...

  11. 玉山黑猪 ESR 和 FSH -β基因多态性及相关性状的研究%Study on Polymorphism of Genes ESR and FSH-βand Related Traits in Yushan Black Pig

    Institute of Scientific and Technical Information of China (English)

    万明春; 周泉勇; 霍俊宏; 李小光

    2014-01-01

    In this study , PCR-RFLP method was used to detect the polymorphisms of genes ESR and FSH-βin Yushan black pigs , and the relationship between the genic polymorphism and reproductive performance was analyzed .The results indicated that the genotypes AA , AB and BB of ESR gene in Yushan black pigs had the frequency of 0.417, 0.300 and 0.017 respectively;the litter size of various genotypes was different , and generally revealed the following sequence:BB>AB>AA.The number of pri-miparous piglets of genotype BB was 1.21 heads more than that of genotype AA , and 0.91 heads more than that of genotype AB . The number of multiparous piglets of BB was also more than that of AB and AA , but there were no significant differences in this a-mong 3 genotypes.The frequency of genotypes AA , AB and BB of FSH-βgene in Yushan black pigs was 0.567, 0.333 and 0. 100 respectively;there were differences in litter size among 3 genotypes, showing the trend of BB >AB>AA in general.The num-ber of primiparous piglets of BB was 0.80 heads more than that of AA (P0. 05).The genotype BB also had the highest multiparous piglets , but there were no significant differences among 3 genotypes.Both ESR gene and FSH-βgene had obvious additive genetic effect in the number of primiparous piglets and number of multiparous pig -lets (P<0.05).%采用PCR-RFLP方法对玉山黑猪的ESR基因和FSH-β基因多态性进行检测,并分析其与繁殖性能间的关系。结果表明,ESR基因的3种基因型AA、AB、BB在玉山黑猪中的频率分别为0.417、0.300、0.017,各基因型对应的产仔数存在差异,总体表现BB>AB>AA,其中初产仔猪数BB型多于AA型1.21头,多于AB型0.91头;BB型经产仔猪数也高于AB型和AA型,但3种基因型之间差异不显著。 FSH-β基因的3种基因型AA、AB、BB在玉山黑猪中的频率分别为0.567、0.333、0.100,各基因型对应的产仔数存在差异,总体表现BB>AB>AA

  12. Avaliação dos níveis basais de FSH em pacientes inférteis com endometriose profunda de ovário tratadas cirurgicamente Evaluation of basal FSH serum levels in infertile patients with deep ovarian endometriosis who underwent surgery

    Directory of Open Access Journals (Sweden)

    Sandra Frankfurt

    2009-07-01

    Full Text Available OBJETIVO: avaliar a reserva ovariana de pacientes inférteis portadoras de endometriose profunda de ovário, submetidas à cirurgia excisional dos endometriomas, atendidas entre os meses de Fevereiro e Novembro de 2008. MÉTODOS: estudo prospectivo que incluiu 30 pacientes portadoras de endometriose graus III e IV com comprometimento profundo de ovário submetidas à cirurgia excisional dos endometriomas e 30 pacientes portadoras de endometriose graus I e II que foram alocadas como Grupo Controle. A reserva ovariana foi avaliada indiretamente a partir do valor do hormônio folículo estimulante (FSH basal (U/L, entre o terceiro e quinto dias do ciclo, após um período de 12 meses da cirurgia. O índice de massa corpórea (IMC foi calculado conforme a fórmula de Quetelet [peso (kg/estatura (cm²]. Para a comparação das variáveis "idade", "IMC" e "valores de FSH basal" entre os grupos, foi utilizado o teste não paramétrico U de Mann-Whitney. RESULTADOS: não foi encontrada diferença significativa entre os grupos em relação à idade e ao IMC. Em relação ao FSH basal, observou-se que, no grupo das pacientes com endometriose profunda, o valor médio foi de 7,0 U/L, enquanto que, no Grupo Controle, foi de 5,6 U/L (p=0,3, o que demonstra que a diferença no valor médio de FSH encontrado nos dois grupos não foi significativa. CONCLUSÕES: a cirurgia não influenciou de forma deletéria a reserva ovariana das pacientes com endometriose profunda de ovário.PURPOSE: to evaluate the ovarian reserve of infertile patients with severe ovarian endrometriosis, submitted to excisional surgery of endometriomas and attended from February to November, 2008. METHODS: prospective study, including 30 patients with endometriosis grades III and IV, with severe ovarian impairment, submitted to excisional surgery of the endometriomas, and 30 patients with endometriosis grades I and II, allocated as a Control Group. The ovarian reserve was indirectly assessed

  13. Lower Hatchie Forest Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Lower Hatchie National Wildlife Refuge Forest Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of...

  14. Montezuma National Wildlife Refuge Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Montezuma National Wildlife Refuge Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern at...

  15. Fish Springs National Wildlife Refuge habitat map

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Habitat map for Fish Springs National Wildlife Refuge. This habitat map was created along with the National Vegetation Classification (NVC) map of the refuge. Refuge...

  16. Morris Wetland Management District Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Morris Wetland Management District Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern at...

  17. Benthic Habitats of the Florida Keys

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The benthic habitats of the Florida Keys were mapped from a series of 450 aerial photographs. Ecologists outlined the boundaries of specific habitat types by...

  18. Muscatatuck National Wildlife Refuge Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Muscatatuck National Wildlife Refuge Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern...

  19. Stream Habitat Reach Summary - NCWAP [ds158

    Data.gov (United States)

    California Department of Resources — The Stream Habitat - NCWAP - Reach Summary [ds158] shapefile contains in-stream habitat survey data summarized to the stream reach level. It is a derivative of the...

  20. AFSC/ABL: Juvenile rockfish habitat utilization

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Juvenile rockfish were observed amongst coral, sponge, cobble, and gravel habitats. Rockfish utilized coral habitats more than any other, while gravel was the least...

  1. Lacassine National Wildlife Refuge Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Lacassine National Wildlife Refuge Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern at...

  2. Fungi from marine habitats: Application in bioremediation

    Digital Repository Service at National Institute of Oceanography (India)

    Raghukumar, C.

    This paper synthesizes the work done on decolourization of bleach plant effluent and synthetic dyes using fungi from marine habitats. Three fungi obtained from marine habitats removed up to 60-95% of Kraft paper mill bleach plant effluent colour...

  3. Chincoteague National Wildlife Refuge Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Chincoteague National Wildlife Refuge Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern...

  4. Agassiz National Wildlife Refuge Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Agassiz National Wildlife Refuge Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern at...

  5. Habitat Appraisal by Vermont Electric Power Company

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Declines in habitat availability have been associated with population declines in bird species breeding in early successional forest and shrubland habitats....

  6. Sabine National Wildlife Refuge Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Sabine NWR Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern at the Refuge, to...

  7. Mandalay National Wildlife Refuges Habitat Management Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Mandalay NWR Habitat Management Plan provides a long-term vision and specific guidance on managing habitats for the resources of concern at the Refuge, to...

  8. Pacific Northwest Salmon Habitat Project Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In the Pacific Northwest Salmon Habitat Project Database Across the Pacific Northwest, both public and private agents are working to improve riverine habitat for a...

  9. Inhibin-B secretion and FSH isoform distribution may play an integral part of follicular selection in the natural menstrual cycle

    DEFF Research Database (Denmark)

    Yding Andersen, C

    2017-01-01

    The aim of the present paper is to expand the concept on how follicular selection takes place in the follicular phase of the natural menstrual cycle. It is suggested that inhibin-B exerts a more intimate role in this process than previously understood. Inhibin-B shows a peak in the circulation....... Collectively, it appears that inhibin-B is an integral part of follicular selection in the normal menstrual cycle, exerting both endocrine and paracrine effects and facilitating continued growth of the selected follicle....... around cycle day 7, simultaneous with selection of the dominant follicle, whereas levels of estradiol and inhibin-A only start to increase a few days later suggesting that inhibin-B is mainly responsible for downregulating pituitary FSH release. New data now demonstrate that the circulatory peak...

  10. A randomized controlled dose-response pilot study of addition of hCG to recombinant FSH during controlled ovarian stimulation for in vitro fertilization.

    Science.gov (United States)

    Thuesen, L L; Loft, A; Egeberg, A N; Smitz, J; Petersen, J H; Andersen, A Nyboe

    2012-10-01

    Is it possible to define an optimal dose of hCG in combination with rFSH from the first day of stimulation in the GnRH agonist protocol applied to IVF? Supplementation with hCG from the first day of stimulation may increase the number of top-quality embryos per patient. Daily doses of hCG up to 150 IU are compatible with good live birth rates. A ceiling level of estradiol (E(2)) was reached with hCG doses above 100 IU/day. A positive dose-response was seen for pre-ovulatory progesterone, but concentrations remained below values for which an impairment of endometrial receptivity has been previously reported. We suggest a large clinical trial to be proceeded with a group given 100 IU hCG daily versus a control group. Prospective multicentre studies have indicated increased live birth rates and increased number of top-quality embryos when low doses of hCG were associated with FSH. We analysed the clinical, embryological and endocrine aspects of adding increasing doses of hCG to rFSH from the first day of stimulation for IVF. A prospective randomized, controlled, open-label dose-response pilot study was conducted between February 2009 and June 2010 at Copenhagen University Hospital, Rigshospitalet, Denmark. Adequate allocation concealment was assured from sequentially numbered, opaque, sealed envelopes prepared from a computer-generated list. Scoring of the embryos was done in an assessor-blinded way. Endocrinologically normal IVF patients aged 25-37 years, BMI 18-30 kg/m(2), regular cycles and FSH D100; n= 16) and Dose 150 (D150; n= 15). Two patients in D150 were withdrawn after randomization because of major (10- to 30-fold) hCG dosing errors, leaving 13 patients in this group. Thus, the results are based on the per protocol population. The mean numbers of top-quality embryos per patient were D0: 0.8 ± 1.2, D50: 0.5 ± 0.7, D100: 1.2 ± 1.7 and D150: 1.5 ± 1.7 (P= 0.04). All pregnancies were singleton gestations, and the live birth rates per started cycle were D0

  11. Eddies reduce denitrification and compress habitats in the Arabian Sea

    Science.gov (United States)

    Lachkar, Zouhair; Smith, Shafer; Lévy, Marina; Pauluis, Olivier

    2016-09-01

    The combination of high biological production and weak oceanic ventilation in regions, such as the northern Indian Ocean and the eastern Pacific and Atlantic, cause large-scale oxygen minimum zones (OMZs) that profoundly affect marine habitats and alter key biogeochemical cycles. Here we investigate the effects of eddies on the Arabian Sea OMZ—the world's thickest—using a suite of regional model simulations with increasing horizontal resolution. We find that isopycnal eddy transport of oxygen to the OMZ region limits the extent of suboxia so reducing denitrification, increasing the supply of nitrate to the surface, and thereby enhancing biological production. That same enhanced production generates more organic matter in the water column, amplifying oxygen consumption below the euphotic zone, thus increasing the extent of hypoxia. Eddy-driven ventilation likely plays a similar role in other low-oxygen regions and thus may be crucial in shaping marine habitats and modulating the large-scale marine nitrogen cycle.

  12. Ghrelin对雌性大鼠促性腺激素分泌及其mRNA表达的影响%Effect of Ghrelin on the secretion of LH,FSH and LH mRNA,FSH mRNA expression in female rat

    Institute of Scientific and Technical Information of China (English)

    王琳; 方富贵; 章孝荣; 刘亚; 张运海; 陶勇; 李运生; 曹鸿国

    2010-01-01

    [目的]探明Ghrelin在中枢水平上对雌性大鼠促性腺激素分泌及其mRNA表达的影响.[方法]用1 nmol Ghrelin对不同发情周期(发情前期、发情期、发情后期、间情期)雌性大鼠及用不同剂量(0.3,1和3 nmol)Ghrelin对发情后期雌性大鼠分别进行侧脑室注射,15 min后断头采血,离心血清,同时采集脑垂体,采用酶免法测定血清促黄体素(LH)、促卵泡素(FSH)的质量浓度,用实时荧光定量PCR法检测LH mRNA、FSH mRNA在垂体中的表达.[结果]①在发情前期和间情期,Ghrelin可显著抑制雌性大鼠LH的分泌和LH mRNA的表达(P<0.05);Ghrelin对整个发情周期中FSH的分泌不产生影响,仅在发情期抑制FSH mRNA的表达(P<0.05).②在发情后期,0.3和1 nmol的Ghrelin对大鼠促性腺激素分泌和mRNA表达均无影响,但3 nmol的Ghrelin可显著抑制LH、FSH的分泌和LH mRNA的表达(P<0.05).[结论]Ghrelin在中枢水平上对FSH、LH的释放及FSH mRNA和LH mRNA的表达均有抑制作用,但存在一定的剂量差异,并受发情周期的调节.

  13. The vallue of AMH,FSH,E2 in POF%AMH、FSH、E2在卵巢早衰诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    徐琳; 任莉; 韩雪松; 刘攀

    2011-01-01

    目的 研究顺铂所致化疗损伤性卵巢早衰大鼠血清AMH、FSH、E2水平变化,探讨血清AMH、FSH、E2能否作为评估成年雌性大鼠卵巢储备的早期预测指标.方法 通过给成熟雌性SD大鼠腹腔注射生理盐水、4.5mg/kg和6.0mg/kg顺铂,建立大鼠化疗损伤性卵巢早衰模型.检测血清AMH、FSH、E2水平.结果 腹腔注射后,大鼠血清AMH水平均明显降低;血清E2水平与对照组相比,低剂量组明显升高,高剂量组无统计学意义,高剂量组与低剂量组相比明显降低;血清FSH水平:低剂量组与时照组相比无统计学意义,高剂量组与对照组、低剂量组相比均升高.腹腔注射前后,各顺铂组血清AMH水平注射后均较注射前明显降低;血清E2水平于注射低剂量顺铂后升高,而注射高剂量顺铂后差异无统计学意义.各顺铂组血清FSH水平注射后均较注射前明显升高.结论 顺铂所致的卵巢损害早期血清E2代偿性升高,AMH明显降低,此两种激素的变化早于FSH的变化.血清E2的升高和AMH的降低可以作为暴露于化疗后的卵巢损害的早期预测指标,两者结合起来将有助于早期诊断卵巢早衰.%Obejective: To study the changes of serum AMH, FSH, E2 in rats of cisplatin - induced premature ovarian failure in order to investigate serum AMH, FSH, E2 which can be assessed to the early prediction indicators of ovarian reserve in adult female rats. Methods: Adult female SD rats were treated with saline, 4. 5mg/kg and 6. 0mg/kg cisplatin by intraperitoneal injection to establish successfully in rat model of chemotherapy induced premature ovarian failure. The serum AMH, FSH, E2 were measured. Results: The serum AMH levels were significantly lower in both cisplatin groups after intraperitoneal injection; The serum E2 levels were significantly higher in low - dose group but there were not significantly in high - dose group compared with the control group. The serum E2 levels were significantly

  14. Spatial variability of Chinook salmon spawning distribution and habitat preferences

    Science.gov (United States)

    Cram, Jeremy M.; Torgersen, Christian; Klett, Ryan S.; Pess, George R.; May, Darran; Pearsons, Todd N.; Dittman, Andrew H.

    2017-01-01

    We investigated physical habitat conditions associated with the spawning sites of Chinook Salmon Oncorhynchus tshawytscha and the interannual consistency of spawning distribution across multiple spatial scales using a combination of spatially continuous and discrete sampling methods. We conducted a census of aquatic habitat in 76 km of the upper main-stem Yakima River in Washington and evaluated spawning site distribution using redd survey data from 2004 to 2008. Interannual reoccupation of spawning areas was high, ranging from an average Pearson’s correlation of 0.62 to 0.98 in channel subunits and 10-km reaches, respectively. Annual variance in the interannual correlation of spawning distribution was highest in channel units and subunits, but it was low at reach scales. In 13 of 15 models developed for individual years (2004–2008) and reach lengths (800 m, 3 km, 6 km), stream power and depth were the primary predictors of redd abundance. Multiple channels and overhead cover were patchy but were important secondary and tertiary predictors of reach-scale spawning site selection. Within channel units and subunits, pool tails and thermal variability, which may be associated with hyporheic exchange, were important predictors of spawning. We identified spawning habitat preferences within reaches and channel units that are relevant for salmonid habitat restoration planning. We also identified a threshold (i.e., 2-km reaches) beyond which interannual spawning distribution was markedly consistent, which may be informative for prioritizing habitat restoration or conservation. Management actions may be improved through enhanced understanding of spawning habitat preferences and the consistency with which Chinook Salmon reoccupy spawning areas at different spatial scales.

  15. Crossing habitat boundaries : mechanisms underlying cross-habitat utilization by reef fishes

    NARCIS (Netherlands)

    Grol, M.G.G.

    2010-01-01

    This thesis contributes to a better understanding of the nursery-role hypothesis of non-reef habitats for coral reef fishes. Not only fish densities were studied in multiple habitats, but also factors which could drive ontogenetic habitat shifts by fishes, such as habitat structural complexity, food

  16. Palmyra Atoll coral reef habitat mapping completion report

    OpenAIRE

    2010-01-01

    The United States Coral Reef Task Force (USCRTF) was established in 1998 by Presidential Executive Order 13089 to lead U.S. efforts to preserve and protect coral reef ecosystems. Current, accurate, and consistent maps greatly enhance efforts to preserve and manage coral reef ecosystems. With comprehensive maps and habitat assessments, coral reef managers can be more effective in designing and implementing a variety of conservation measures, including: • Long-term monitoring programs with ...

  17. Light indirectly mediates bivalve habitat modification and impacts on seagrass

    DEFF Research Database (Denmark)

    Castorani, Max Christopher Nicholas; Glud, Ronnie N.; Hasler-Sheetal, Harald

    2015-01-01

    and plant responseswere measured. Light modified the effect of mussels on porewater ammonium, but eelgrass was not nutrient limited and, therefore, mussels did not enhance growth. Mussels increased sediment sulfides irrespective of light availability and, by reducing net oxygen flux (production...... and respiration),mussels and lowlight availability exacerbated sulfide intrusion of eelgrass tissues. Surprisingly, sulfide stress did not affect plant growth, survival, or energy stores. Thus, habitat modification by musselsmay represent a risk to eelgrass, especially during low productivity conditions...

  18. Molecular cloning of a novel, putative G protein-coupled receptor from sea anemones structurally related to members of the FSH, TSH, LH/CG receptor family from mammals

    DEFF Research Database (Denmark)

    Nothacker, H P; Grimmelikhuijzen, C J

    1993-01-01

    hormone (FSH, TSH, LH/CG) receptor family from mammals, including a very large, extracellular N terminus (18-25% sequence identity) and a 7 transmembrane region (44-48% sequence identity). As with the mammalian glycoprotein hormone receptor genes, the sea anemone receptor gene yields transcripts which can...

  19. Ovarian response and pregnancy outcome related to mid-follicular LH levels in women undergoing assisted reproduction with GnRH agonist down-regulation and recombinant FSH stimulation

    DEFF Research Database (Denmark)

    Humaidan, P; Bungum, L; Bungum, M

    2002-01-01

    stimulation with recombinant FSH. METHODS: Blood samples were prospectively collected from a total of 207 normal women undergoing assisted reproduction and analysed retrospectively. Based on LH levels on stimulation day 8 patients were divided into four groups: 1.51 IU/l. RESULTS...

  20. Habitat Evaluation Procedures (HEP) Report; Tacoma Creek South Project, Technical Report 2003-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On July 6, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Tacoma Creek South property, an acquisition completed by the Kalispel Tribe of Indians in June 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Tacoma Creek South Project provides a total of 190.79 Habitat Units (HUs) for the species evaluated. Emergent wetlands provide 20.51 HUs for Canada goose, mallard, and muskrat. Grassland provides 1.65 HUs for Canada goose and mallard. Scrub-shrub vegetation provides 11.76 HUs for mallard, yellow warbler, and white-tailed deer. Conifer forest habitat provides 139.92 HUs for bald eagle, black-capped chickadee, and white-tailed deer. Deciduous forest also provides 19.15 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. The objective of using HEP at the Tacoma Creek South Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  1. Habitat Evaluation Procedures (HEP) Report; Upper Trimble Project, Technical Report 2004-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On July 13, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Upper Trimble property, an acquisition completed by the Kalispel Tribe of Indians in March 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Upper Trimble Project provides a total of 250.67 Habitat Units (HUs) for the species evaluated. Wet meadow provides 136.92 HUs for mallard, muskrat, and Canada goose. Mixed forest habitat provides 111.88 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Scrub-shrub vegetation provides 1.87 HUs for yellow warbler, and white-tailed deer. The objective of using HEP at the Upper Trimble Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  2. Assessments of habitat preferences and quality depend on spatial scale and metrics of fitness

    Science.gov (United States)

    Chalfoun, A.D.; Martin, T.E.

    2007-01-01

    1. Identifying the habitat features that influence habitat selection and enhance fitness is critical for effective management. Ecological theory predicts that habitat choices should be adaptive, such that fitness is enhanced in preferred habitats. However, studies often report mismatches between habitat preferences and fitness consequences across a wide variety of taxa based on a single spatial scale and/or a single fitness component. 2. We examined whether habitat preferences of a declining shrub steppe songbird, the Brewer's sparrow Spizella breweri, were adaptive when multiple reproductive fitness components and spatial scales (landscape, territory and nest patch) were considered. 3. We found that birds settled earlier and in higher densities, together suggesting preference, in landscapes with greater shrub cover and height. Yet nest success was not higher in these landscapes; nest success was primarily determined by nest predation rates. Thus landscape preferences did not match nest predation risk. Instead, nestling mass and the number of nesting attempts per pair increased in preferred landscapes, raising the possibility that landscapes were chosen on the basis of food availability rather than safe nest sites. 4. At smaller spatial scales (territory and nest patch), birds preferred different habitat features (i.e. density of potential nest shrubs) that reduced nest predation risk and allowed greater season-long reproductive success. 5. Synthesis and applications. Habitat preferences reflect the integration of multiple environmental factors across multiple spatial scales, and individuals may have more than one option for optimizing fitness via habitat selection strategies. Assessments of habitat quality for management prescriptions should ideally include analysis of diverse fitness consequences across multiple ecologically relevant spatial scales. ?? 2007 The Authors.

  3. Morphological variation and habitat modification are strongly correlated for the autogenic ecosystem engineer Spartina anglica (common cordgrass)

    NARCIS (Netherlands)

    van Hulzen, J.B.; Van Soelen, J.; Bouma, T.J.

    2007-01-01

    We explored to what extent morphological variation and habitat modification are correlated for an autogenic ecosystem engineer, which is an organism that modifies its habitat via its own physical structures. The intertidal salt marsh species Spartina anglica is well known for its capacity to enhance

  4. Reproductive performance of donor mares subsequent to eFSH treatment in early vernal transition: Comparison between the first, second, and mid-season estrous cycles of the breeding season.

    Science.gov (United States)

    Raz, Tal; Hunter, Barbara; Carley, Sylvia; Card, Claire

    2009-11-01

    The objective was to compare the reproductive performances associated with the first (Cycle-1), second (Cycle-2), and mid-season (MS-Cycle) ovulations of the breeding season in donor mares that were treated with equine-FSH (eFSH) in the early vernal transition. Mares (n=15) kept under ambient light were examined ultrasonographically per-rectum starting January 30. When an ovarian follicle > or =25mm in diameter was detected, twice daily eFSH treatments were initiated. The eFSH treatments ceased when a follicle > or =35mm was detected, and 36h later hCG was administered. Thereafter, mares were artificially inseminated every 48h until ovulation (Day 0). Trans-cervical embryo recovery attempts were performed on Day 8, and subsequently PGF2alpha was administered. Equine FSH was not administered in the subsequent estrous cycles. In Cycle-2 and in the MS-Cycle, hCG was administered when a follicle > or =35mm was detected; breeding, embryo recovery, and PGF2alpha administration, were similar to Cycle-1. Mares had an untreated estrous cycle (no treatment or breeding) between Cycle-2 and the MS-Cycle. All mares developed follicle(s) > or =35mm after 4.9+/-0.6 days of eFSH treatment, and subsequently ovulations occurred; mean (95% CI) interval from treatment initiation to ovulation was 7.9 (6.5-9.3) days. The number of preovulatory follicles (> or =30mm) at the time of hCG administration (Cycle-1: 2.2+/-0.3 compared with Cycle-2: 1.0+/-0 compared with MS-Cycle: 1.1+/-0.1 follicles), and the number of ovulations (2.5+/-0.4 compared with 1.0+/-0 compared with 1.1+/-0.1 ovulations) were greater (phormonal environment (estradiol-17beta and progesterone). A prolonged IOI (>21 days) was recorded in 7 of 15 mares following the Cycle-1 ovulation, but not subsequently. In conclusion, eFSH treatment of vernal transitional donor mares stimulated ovulation within only few days of treatment, and the following embryo recovery rate was at least as good as in the subsequent estrous cycles

  5. Instream Physical Habitat Modelling Types

    DEFF Research Database (Denmark)

    Conallin, John; Boegh, Eva; Krogsgaard, Jørgen

    2010-01-01

    -friendly and have flexible data needs. They can easily be implemented in new regions using expert information or different types of local data. Furthermore, they are easily presentable to stakeholders and have the potential to be applied over large spatial scales. Integral care must be taken in the use...... and disadvantages as management tools for member states in relation to the requirements of the WFD, but due to their different model structures they are distinct in their data needs, transferability, user-friendliness and presentable outputs. Water resource managers need information on what approaches will best...... management tools, but require large amounts of data and the model structure is complex. It is concluded that the use of habitat suitability indices (HSIs) and fuzzy rules in hydraulic-habitat modelling are the most ready model types to satisfy WFD demands. These models are well documented, transferable, user...

  6. An approach of habitat degradation assessment for characterization on coastal habitat conservation tendency.

    Science.gov (United States)

    Zhou, Xi-Yin; Lei, Kun; Meng, Wei

    2017-09-01

    Coastal zones are population and economy highly intensity regions all over the world, and coastal habitat supports the sustainable development of human society. The accurate assessment of coastal habitat degradation is the essential prerequisite for coastal zone protection. In this study, an integrated framework of coastal habitat degradation assessment including landuse classification, habitat classifying and zoning, evaluation criterion of coastal habitat degradation and coastal habitat degradation index has been established for better regional coastal habitat assessment. Through establishment of detailed three-class landuse classification, the fine landscape change is revealed, the evaluation criterion of coastal habitat degradation through internal comparison based on the results of habitat classifying and zoning could indicate the levels of habitat degradation and distinguish the intensity of human disturbances in different habitat subareas under the same habitat classification. Finally, the results of coastal habitat degradation assessment could be achieved through coastal habitat degradation index (CHI). A case study of the framework is carried out in the Circum-Bohai-Sea-Coast, China, and the main results show the following: (1) The accuracy of all land use classes are above 90%, which indicates a satisfactory accuracy for the classification map. (2) The Circum-Bohai-Sea-Coast is divided into 3 kinds of habitats and 5 subareas. (3) In the five subareas of the Circum-Bohai-Sea-Coast, the levels of coastal habitat degradation own significant difference. The whole Circum-Bohai-Sea-Coast generally is in a worse state according to area weighting of each habitat subarea. This assessment framework of coastal habitat degradation would characterize the landuse change trend, realize better coastal habitat degradation assessment, reveal the habitat conservation tendency and distinguish intensity of human disturbances. Furthermore, it would support for accurate coastal

  7. Modelling Fish Habitat Suitability in the Eastern English Channel. Application to community habitat level

    OpenAIRE

    Vaz, Sandrine; Carpentier, Andre; Loots, Christophe; Koubbi, Philippe

    2004-01-01

    Valuable marine habitats and living resources can be found in the Eastern English Channel and in 2003, a Franco-British Interreg IIIA project, ‘Eastern Channel Habitat Atlas for Marine Resource Management’ (CHARM), was initiated to support decision-making for management of essential fish habitats. Fish habitat corresponds to geographic areas within which ranges of environmental factors define the presence of a particular species. Habitat Suitability index (HSI) modelling was used to relate fi...

  8. Carpinteria salt marsh habitat polygons

    Science.gov (United States)

    Lafferty, Kevin D.; Dunham, Eleca J.; Mancini, Frank T.; Stewart, Tara E.; Hechinger, Ryan F.

    2017-01-01

    We identified five common habitat types in Carpinteria Salt Marsh: channels, pans (flats), marsh, salt flat and upland.  We then drew polygons around each habitat type identified from a registered and orthorectified aerial photograph and created a GIS shapefile. Polygons were ground-truthed in the field. From these habitat polygons, one can use GIS applications to estimate the area of each habitat type in this estuary. These data support the following publications: Kuris, Armand M., et al. "Ecosystem energetic implications of parasite and free-living biomass in three estuaries." Nature 454.7203 (2008): 515-518.Hechinger, Ryan F., Kevin D. Lafferty, Andy P. Dobson, James H. Brown, and Armand M. Kuris. "A common scaling rule for abundance, energetics, and production of parasitic and free-living species." Science 333, no. 6041 (2011): 445-448.Hechinger, Ryan F., Kevin D. Lafferty, John P. McLaughlin, Brian L. Fredensborg, Todd C. Huspeni, Julio Lorda, Parwant K. Sandhu et al. "Food webs including parasites, biomass, body sizes, and life stages for three California/Baja California estuaries." Ecology 92, no. 3 (2011): 791-791.Buck, J.C., Hechinger, R.F., Wood, A.C., Stewart, T.E., Kuris, A.M., and Lafferty, K.D., "Host density increases parasite recruitment but decreases host risk in a snail-trematode system." Manuscript submitted for publication. Lafferty, K.D., Stewart, T.E., and Hechinger, R.F. (in press). Bird distribution surveys at Carpinteria Salt Marsh, California USA, January 2012 to March 2013: U.S. Geological Survey data release, http://dx.doi.org/10.5066/F7F47M95. 

  9. Ecology and habitats of extremophiles.

    Science.gov (United States)

    Kristjánsson, J K; Hreggvidsson, G O

    1995-01-01

    This review describes the main natural extreme environments, characterized by high temperature, high and low pH and high salinity, that can be colonized by microorganisms. The environments covered are: freshwater alkaline hot springs; acidic solfatara fields; anaerobic geothermal mud and soils; acidic sulphur and pyrite areas; carbonate springs and alkaline soil; and soda and highly saline lakes. The community structure, in terms of available energy sources and representative autotrophic and heterotrophic microorganisms, is discussed for each type of habitat.

  10. Habitat Technology Research at DLR

    OpenAIRE

    Quantius, Dominik; Schubert, Daniel; Maiwald, Volker; Hauslage, Jens; Bornemann, Gerhild; Waßer, Kai; Hill, Jürgen; Henn, Norbert; Ruyters, Hans-Günter; Braun, Markus

    2013-01-01

    For long duration space missions a closed-loop system which can re-use of materials is mandatory. Also on Earth there are harsh environments or overpopulated areas where a sustainable handling of given goods is indispensable. Addressing these challenges the German Aerospace Center (DLR) conducts research in various fields of habitat technology development, which will be illustrated within this paper. There are various complementary topics, such as coordination and funding of building blocks f...

  11. Integration Process for the Habitat Demonstration Unit

    Science.gov (United States)

    Gill, Tracy; Merbitz, Jerad; Kennedy, Kriss; Tn, Terry; Toups, Larry; Howe, A. Scott; Smitherman, David

    2011-01-01

    maximize the efficiency of both integration and field operations. In addition a series of tailored design reviews are utilized to quickly define the systems and their integration into the DSH configuration. These processes are necessary to ensure activities, such as partially reversing integration of the X-Hab second story of the HDU and deploying and stowing the new work porch for transportation to the JSC Rock Yard and to the Arizona Black Point Lava Flow Site are performed with minimal or no complications. In addition, incremental test operations leading up to an Integrated systems test allows for an orderly systems test program. For FY-11 activities, the HDU DSH will act as a laboratory utilizing a new X-Hab inflatable second floor with crew habitation features. In addition to the day to day operations involving maintenance of the HDU and exploring the surrounding terrain, testing and optimizing the use of the new X-Hab, work porch, Hygiene Module, and other sub-system enhancements will be the focus of the FY-11 test objectives. The HDU team requires a successful integration strategy using a variety of tools and approaches to prepare the DSH for these test objectives. In a challenging environment where the prototyping influences the system design, as well as Vice versa, results of the HDU DSH field tests will influence future designs of habitat systems.

  12. Rainwater Wildlife Area Habitat Evaluation Procedures Report; A Columbia Basin Wildlife Mitigation Project.

    Energy Technology Data Exchange (ETDEWEB)

    Childs, Allen B.

    2004-01-01

    vegetation species, allowance of normative processes such as fire occurrence, and facilitating development of natural stable stream channels and associated floodplains. Implementation of habitat enhancement and restoration activities could generate an additional 1,850 habitat units in 10 years. Baseline and estimated future habitat units total 7,035.3 for the Rainwater Wildlife Area. Habitat protection, enhancement and restoration will require long-term commitments from managers to increase probabilities of success and meet the goals and objectives of the Northwest Power Planning Council's Fish and Wildlife Mitigation Program.

  13. Habitat evaluation using GIS a case study applied to the San Joaquin Kit Fox

    Science.gov (United States)

    Gerrard, R.; Stine, P.; Church, R.; Gilpin, M.

    2001-01-01

    Concern over the fate of plant and animal species throughout the world has accelerated over recent decades. Habitat loss is considered the main culprit in reducing many species' abundance and range, leading to numerous efforts to plan and manage habitat preservation. Our work uses Geographic Information Systems (GIS) data and modeling to define a spatially explicit analysis of habitat value, using the San Joaquin Kit Fox (Vulpes macrotis mutica) of California (USA) as an example. Over the last 30 years, many field studies and surveys have enhanced our knowledge of the life history, behavior, and needs of the kit fox, which has been proposed as an umbrella or indicator species for grassland habitat in the San Joaquin Valley of California. There has yet been no attempt to convert much of this field knowledge into a model of spatial habitat value useful for planning purposes. This is a significant omission given the importance and visibility of the imperiled kit fox and increasing trends toward spatially explicit modeling and planning. In this paper we apply data from northern California to derive a small-cell GIS raster of habitat value for the kit fox that incorporates both intrinsic habitat quality and neighborhood context, as well the effects of barriers such as roads. Such a product is a useful basis for assessing the presence and amounts of good (and poor) quality habitat and for eventually constructing GIS representations of viable animal territories that could be included in future reserves. ?? 2001 Elsevier Science B.V.

  14. Urinary hMG versus recombinant FSH for controlled ovarian hyperstimulation following an agonist long down-regulation protocol in IVF or ICSI treatment: a systematic review and meta-analysis.

    Science.gov (United States)

    Coomarasamy, Arri; Afnan, Masoud; Cheema, Deepti; van der Veen, Fulco; Bossuyt, Patrick M M; van Wely, Madelon

    2008-02-01

    Since the most recent Cochrane review on hMG versus rFSH for controlled ovarian hyperstimulation following a long down-regulation protocol, several new trials have emerged. We conducted a systematic review and meta-analysis of randomized trials comparing the effectiveness of hMG versus rFSH following a long down-regulation protocol in IVF-ICSI cycles, on the primary outcome of live birth per woman randomized, as well as several other secondary outcomes. Searches were conducted in MEDLINE, EMBASE, Science Direct, Cochrane Library and databases of abstracts (last search January 2007). Seven randomized trials, consisting of a total of 2159 randomized women, were identified. A meta-analysis of these trials showed a significant increase in live birth rate with hMG when compared with rFSH (relative risk, RR = 1.18, 95% CI: 1.02-1.38, P = 0.03). The heterogeneity test was non-significant (P = 0.97), suggesting that there was no statistical inconsistency between the seven studies. The pooled risk difference (RD) for the outcome of live birth rate was 4% (95% CI: 1-7%) for these study populations. There was an increase in clinical pregnancy rates with hMG when compared with rFSH (RR = 1.17, 95% CI 1.03-1.34). No significant differences were noted for gonadotrophin use, spontaneous abortion, multiple pregnancy, cancellation and ovarian hyperstimulation syndrome rates. For the populations in the randomized trials, hMG was associated with a pooled 4% increase in live birth rate when compared with rFSH in IVF-ICSI treatment following a long down-regulation protocol.

  15. Economic efficiency and cost implications of habitat conservation: An example in the context of the Edwards Aquifer region

    Science.gov (United States)

    Gillig, Dhazn; McCarl, Bruce A.; Jones, Lonnie L.; Boadu, Frederick

    2004-04-01

    Groundwater management in the Edwards Aquifer in Texas is in the process of moving away from a traditional right of capture economic regime toward a more environmentally sensitive scheme designed to preserve endangered species habitats. This study explores economic and environmental implications of proposed groundwater management and water development strategies under a proposed regional Habitat Conservation Plan. Results show that enhancing the habitat by augmenting water flow costs $109-1427 per acre-foot and that regional water development would be accelerated by the more extreme possibilities under the Habitat Conservation Plan. The findings also indicate that a water market would improve regional welfare and lower water development but worsen environmental attributes.

  16. A habitat overlap analysis derived from Maxent for Tamarisk and the South-western Willow Flycatcher

    Institute of Scientific and Technical Information of China (English)

    Patricia YORK; Paul EVANGELISTA; Sunil KUMAR; James GRAHAM; Curtis FLATHER; Thomas STOHLGREN

    2011-01-01

    Biologic control of the introduced and invasive,woody plant tamarisk (Tamarix spp,saltcedar) in south-western states is controversial because it affects habitat of the federally endangered South-western Willow Flycatcher (Empidonax traillii extimus).These songbirds sometimes nest in tamarisk where floodplain-level invasion replaces native habitats.Biologic control,with the saltcedar leaf beetle (Diorhabda elongate),began along the Virgin River,Utah,in 2006,enhancing the need for comprehensive understanding of the tamarisk-flycatcherrelationship.We used maximum entropy (Maxent) modeling to separately quantify the current extent of dense tamarisk habitat ( > 50% cover) and the potential extent of habitat available for E.traillii extimus within the studied watersheds.We used transformations of 2008 Landsat Thematic Mapper images and a digital elevation model as environmental input variables.Maxent models performed well for the flycatcher and tamarisk with Area Under the ROC Curve (AUC) values of 0.960 and 0.982,respectively.Classification of thresholds and comparison of the two Maxent outputs indicated moderate spatial overlap between predicted suitable habitat for E.traillii extimus and predicted locations with dense tamarisk stands,where flycatcher habitat will potentially change flycatcher habitats.Dense tamarisk habitat comprised 500 km2 within the study area,of which 11.4% was also modeled as potential habitat for E.traillii extimus.Potential habitat modeled for the flycatcher constituted 190 km2,of which 30.7% also contained dense tamarisk habitat.Results showed that both native vegetation and dense tamarisk habitats exist in the study area and that most tamarisk infestations do not contain characteristics that satisfy the habitat requirements of E.traillii extimus.Based on this study,effective biologic control of Tamarix spp.may,in the short term,reduce suitable habitat available to E.traillii extimus,but also has the potential in the long term to increase

  17. Yakima Habitat Improvement Project Master Plan, Technical Report 2003.

    Energy Technology Data Exchange (ETDEWEB)

    Golder Associates, Inc.

    2003-04-22

    The Yakima Urban Growth Area (UGA) is a developing and growing urban area in south-central Washington. Despite increased development, the Yakima River and its tributaries within the UGA continue to support threatened populations of summer steelhead and bull trout as well as a variety of non-listed salmonid species. In order to provide for the maintenance and recovery of these species, while successfully planning for the continued growth and development within the UGA, the City of Yakima has undertaken the Yakima Habitat Improvement Project. The overall goal of the project is to maintain, preserve, and restore functioning fish and wildlife habitat within and immediately surrounding the Yakima UGA over the long term. Acquisition and protection of the fish and wildlife habitat associated with key properties in the UGA will prevent future subdivision along riparian corridors, reduce further degradation or removal of riparian habitat, and maintain or enhance the long term condition of aquatic habitat. By placing these properties in long-term protection, the threat of development from continued growth in the urban area will be removed. To most effectively implement the multi-year habitat acquisition and protection effort, the City has developed this Master Plan. The Master Plan provides the structure and guidance for future habitat acquisition and restoration activities to be performed within the Yakima Urban Area. The development of this Master Plan also supports several Reasonable and Prudent Alternatives (RPAs) of the NOAA Fisheries 2000 Biological Opinion (BiOp), as well as the Water Investment Action Agenda for the Yakima Basin, local planning efforts, and the Columbia Basin Fish and Wildlife Authority's 2000 Fish and Wildlife Program. This Master Plan also provides the framework for coordination of the Yakima Habitat Improvement Project with other fish and wildlife habitat acquisition and protection activities currently being implemented in the area. As a

  18. Camas Creek (Meyers Cove) Anadromous Species Habitat Improvement: Annual Report 1990.

    Energy Technology Data Exchange (ETDEWEB)

    Seaberg, Glen

    1990-06-01

    Populations of wild salmon and steelhead in the Middle Fork of the Salmon River are at historical lows. Until passage and flow problems associated with Columbia River dams are corrected to reduce mortalities of migrating smolts, continuance of habitat enhancements that decrease sediment loads, increase vegetative cover, remove passage barriers, and provide habitat diversity is imperative to maintain surviving populations of these specially adapted fish. In 1987-1988, 4.3 miles of fence was constructed establishing a riparian livestock exclosure. One end-gap and two water-crossing corridors were constructed in 1989 to complete the fence system. Areas within the exclosure have been fertilized to promote tree and shrub root growth and meadow recovery. A stream crossing ford was stabilized with angular cobble. Streambank stabilization/habitat cover work was completed at three sites and three additional habitat structures were placed. Extensive inventories were completed to identify habitat available to anadromous fish. Streambank stabilization work was limited to extremely unstable banks, minimizing radical alterations to an active stream channel. Enhancement activities will improve spawning, incubation, and rearing habitat for wild populations of steelhead trout and chinook salmon. Anadromous species population increases resulting from these enhancement activities will provide partial compensation for downstream losses resulting from hydroelectric developments on the Columbia River system. 10 refs., 11 figs., 5 tabs.

  19. Connecting the fragmented habitat of endangered mammals in the landscape of Riau–Jambi–Sumatera Barat (RIMBA, central Sumatra, Indonesia (connecting the fragmented habitat due to road development

    Directory of Open Access Journals (Sweden)

    Barano Siswa Sulistyawan

    2017-01-01

    Full Text Available The trend of wildlife habitat fragmentation worldwide continues as a result of anthropogenic activities on development of a linear infrastructure and land use changes, which is often implemented as part of spatial planning policies. In this paper we expand upon an existing approach to design wildlife corridors through habitat quality assessment. We used models of Habitat Quality of Integrated Valuation of Ecosystem Services and Trade-offs (InVEST and Corridor Design tools. The habitat quality model of InVEST provides a rapid approach to assess status and change of biodiversity, and can contribute to enhanced corridor design of fragmented wildlife habitat. We conducted an assessment of habitat quality of the RIMBA corridor landscape, which is part of Riau, Jambi and West Sumatra provinces of central Sumatra Island. The result of the habitat quality model was used as the main input to evaluate habitat connectivity and assess the target segment of roads that cross the modelled corridor. We found 20 wildland blocks, the total area of the corridor modelled including wildland blocks was calculated as about 0.77 million hectares. We have obtained accurate quantitative measurement of the length of roads crossing the corridor, with a total of 417.78 km (artery 10.31 km; collector 19.52 km; and local 1987.9 km roads. This method can be replicated as an approach in valuing the quality of habitat as part of the implementation of the presidential decree of Sumatra Island Spatial Planning. This may also be applied to the spatial planning of other major islands in Indonesia and elsewhere.

  20. Julia Butler Hansen NWR: Initial Survey Instructions for Columbian White-tailed Deer Habitat Use and Home Range Monitoring

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Refuge engages in active habitat management for CWTD, including pasture enhancement, grazing, tree planting, and invasive species management. While we assume...

  1. Habitat heterogeneity affects ecological functions of macrobenthic communities in a mangrove: Implication for the impact of restoration and afforestation

    Directory of Open Access Journals (Sweden)

    Jonathan Y.S. Leung

    2015-07-01

    Full Text Available Mangroves have been dwindling rapidly in the last few decades due to human activities, and thus restoration is commonly conducted to recover the ecological functions of degraded mangroves. However, afforestation (i.e. mangrove plantation in mudflats can lead to habitat conversion and hence modify the ecosystem functions by increasing habitat heterogeneity. Mudflats are scarce in mangroves, but provide vital ecological functions by the macrobenthos. As such, the present study investigated how habitat heterogeneity affects the ecological functions of macrobenthic communities in a mangrove by analysing functional diversity, functional redundancy and biological trait patterns. Samples were collected from different habitats with increasing order of habitat heterogeneity (mudflat habitat heterogeneity primarily due to the dense root structure. Different habitats had different trait patterns where smaller body size, fewer carnivores and more direct developers were observed when habitat heterogeneity increased. Overall, moderate increase in habitat heterogeneity enhanced the functional diversity of macrobenthic communities, but negative effect was incurred when the habitat heterogeneity was too high. For the sake of conservation and management of mangroves, restoration should consider plant density and plant species to minimize the impact of dense root structures on macrobenthos. Given the lower functional redundancy and distinct trait pattern in the mudflat, afforestation is not recommended so that the integrity of the ecological functions of mangroves can be maintained.

  2. Lunar base habitat designs: Characterizing the environment, and selecting habitat designs for future trade-offs

    Science.gov (United States)

    Ganapathi, Gani B.; Ferrall, Joseph; Seshan, P. K.

    1993-01-01

    A survey of distinct conceptual lunar habitat designs covering the pre- and post-Apollo era is presented. The impact of the significant lunar environmental challenges such as temperature, atmosphere, radiation, soil properties, meteorites, and seismic activity on the habitat design parameters are outlined. Over twenty habitat designs were identified and classified according to mission type, crew size; total duration of stay, modularity, environmental protection measures, and emplacement. Simple selection criteria of (1) post-Apollo design, (2) uniqueness of the habitat design, (3) level of thoroughness in design layout, (4) habitat dimensions are provided, and (5) materials of construction for the habitat shell are specified, are used to select five habitats for future trade studies. Habitat emplacement scenarios are created to examine the possible impact of emplacement of the habitat in different locations, such as lunar poles vs. equatorial, above ground vs. below ground, etc.

  3. Habitat associations of juvenile versus adult butterflyfishes

    Science.gov (United States)

    Pratchett, M. S.; Berumen, M. L.; Marnane, M. J.; Eagle, J. V.; Pratchett, D. J.

    2008-09-01

    Many coral reef fishes exhibit distinct ontogenetic shifts in habitat use while some species settle directly in adult habitats, but there is not any general explanation to account for these differences in settlement strategies among coral reef fishes. This study compared distribution patterns and habitat associations of juvenile (young of the year) butterflyfishes to those of adult conspecifics. Three species, Chaetodon auriga, Chaetodon melannotus, and Chaetodon vagabundus, all of which have limited reliance on coral for food, exhibited marked differences in habitat association of juvenile versus adult individuals. Juveniles of these species were consistently found in shallow-water habitats, whereas adult conspecifics were widely distributed throughout a range of habitats. Juveniles of seven other species ( Chaetodon aureofasciatus, Chaetodon baronessa, Chaetodon citrinellus, Chaetodon lunulatus, Chaetodon plebeius, Chaetodon rainfordi, and Chaetodon trifascialis), all of which feed predominantly on live corals, settled directly into habitat occupied by adult conspecifics. Butterflyfishes with strong reliance on corals appear to be constrained to settle in habitats that provide access to essential prey resources, precluding their use of distinct juvenile habitats. More generalist butterflyfishes, however, appear to utilize distinct juvenile habitats and exhibit marked differences in the distribution of juveniles versus adults.

  4. 2004 assessment of habitat improvements in Dinosaur Reservoir

    Energy Technology Data Exchange (ETDEWEB)

    Blackman, B.G.; Cowie, D.M.

    2005-01-15

    Formed in 1979 after the completion of the Peace Canyon Dam, Dinosaur Reservoir is 21 km long and backs water up to the tailrace of W.A.C. Bennett Dam. BC Hydro has funded studies to evaluate fish stocking programs and assess habitat limitations and potential enhancements as part of a water licence agreement. The Peace/Williston Fish and Wildlife Compensation Programs (PWFWCP) have undertaken a number of projects to address fish habitat limitations, entrainment and stocking assessments as a result of recommendations stemming from these studies. It was determined that existing baseline fish data was needed in order to evaluate the effectiveness of these activities. A preliminary boat electro-fishing program which was started in October 2001, noted that a propensity for rainbow trout to concentrate near woody debris. In response, a program was started in 2002 to add woody debris to embayment areas throughout the reservoir. These enhanced woody debris structures are located in small sheltered bays and consist of a series of large trees cabled together and anchored to the shore. The area between the cabled trees and the shoreline is filled with woody debris and root wads collected from along the shoreline. The 2004 assessment of habitat improvements in Dinosaur Reservoir presents the findings from a study that compares the number of fish captured using trap nets, angling, and minnow traps, at the woody debris structures to sites with similar physical characteristics where woody debris had not been added. 17 refs., 5 tabs., 4 figs.

  5. Lemming winter habitat choice: a snow-fencing experiment.

    Science.gov (United States)

    Reid, Donald G; Bilodeau, Frédéric; Krebs, Charles J; Gauthier, Gilles; Kenney, Alice J; Gilbert, B Scott; Leung, Maria C-Y; Duchesne, David; Hofer, Elizabeth

    2012-04-01

    The insulative value of early and deep winter snow is thought to enhance winter reproduction and survival by arctic lemmings (Lemmus and Dicrostonyx spp). This leads to the general hypothesis that landscapes with persistently low lemming population densities, or low amplitude population fluctuations, have a low proportion of the land base with deep snow. We experimentally tested a component of this hypothesis, that snow depth influences habitat choice, at three Canadian Arctic sites: Bylot Island, Nunavut; Herschel Island, Yukon; Komakuk Beach, Yukon. We used snow fencing to enhance snow depth on 9-ha tundra habitats, and measured the intensity of winter use of these and control areas by counting rodent winter nests in spring. At all three sites, the density of winter nests increased in treated areas compared to control areas after the treatment, and remained higher on treated areas during the treatment. The treatment was relaxed at one site, and winter nest density returned to pre-treatment levels. The rodents' proportional use of treated areas compared to adjacent control areas increased and remained higher during the treatment. At two of three sites, lemmings and voles showed significant attraction to the areas of deepest snow accumulation closest to the fences. The strength of the treatment effect appeared to depend on how quickly the ground level temperature regime became stable in autumn, coincident with snow depths near the hiemal threshold. Our results provide strong support for the hypothesis that snow depth is a primary determinant of winter habitat choice by tundra lemmings and voles.

  6. Human habitation field study of the Habitat Demonstration Unit (HDU)

    Science.gov (United States)

    Litaker, Harry L.; Archer, Ronald D.; Szabo, Richard; Twyford, Evan S.; Conlee, Carl S.; Howard, Robert L.

    2013-10-01

    Landing and supporting a permanent outpost on a planetary surface represents humankind's capability to expand its own horizons and challenge current technology. With this in mind, habitability of these structures becomes more essential given the longer durations of the missions. The purpose of this evaluation was to obtain preliminary human-in-the-loop performance data on the Habitat Demonstration Unit (HDU) in a Pressurized Excursion Module (PEM) configuration during a 14-day simulated lunar exploration field trial and to apply this knowledge to further enhance the habitat's capabilities for forward designs. Human factors engineers at the NASA/Johnson Space Center's Habitability and Human Factors Branch recorded approximately 96 h of crew task performance with four work stations. Human factors measures used during this study included the NASA Task Load Index (TLX) and customized post questionnaires. Overall the volume for the PEM was considered acceptable by the crew; however; the habitat's individual work station volume was constrained when setting up the vehicle for operation, medical operations, and suit maintenance while general maintenance, logistical resupply, and geo science was considered acceptable. Crew workload for each station indicated resupply as being the lowest rated, with medical operations, general maintenance, and geo science tasks as being light, while suit maintenance was considered moderate and general vehicle setup being rated the highest. Stowage was an issue around the habitat with the Space Exploration Vehicle (SEV) resupply stowage located in the center of the habitat as interfering with some work station volumes and activities. Ergonomics of the geo science station was considered a major issue, especially with the overhead touch screens.

  7. Cost-effectiveness analysis of sandhill crane habitat management

    Science.gov (United States)

    Kessler, Andrew C.; Merchant, James W.; Shultz, Steven D.; Allen, Craig R.

    2013-01-01

    Invasive species often threaten native wildlife populations and strain the budgets of agencies charged with wildlife management. We demonstrate the potential of cost-effectiveness analysis to improve the efficiency and value of efforts to enhance sandhill crane (Grus canadensis) roosting habitat. We focus on the central Platte River in Nebraska (USA), a region of international ecological importance for migrating avian species including sandhill cranes. Cost-effectiveness analysis is a valuation process designed to compare alternative actions based on the cost of achieving a pre-determined objective. We estimated costs for removal of invasive vegetation using geographic information system simulations and calculated benefits as the increase in area of sandhill crane roosting habitat. We generated cost effectiveness values for removing invasive vegetation on 7 land parcels and for the entire central Platte River to compare the cost-effectiveness of management at specific sites and for the central Platte River landscape. Median cost effectiveness values for the 7 land parcels evaluated suggest that costs for creating 1 additional hectare of sandhill crane roosting habitat totaled US $1,595. By contrast, we found that creating an additional hectare of sandhill crane roosting habitat could cost as much as US $12,010 for some areas in the central Platte River, indicating substantial cost savings can be achieved by using a cost effectiveness analysis to target specific land parcels for management. Cost-effectiveness analysis, used in conjunction with geographic information systems, can provide decision-makers with a new tool for identifying the most economically efficient allocation of resources to achieve habitat management goals.

  8. Natural Propagation and Habitat Improvement, Volume 2, Idaho, 1984 Final and Annual Reports.

    Energy Technology Data Exchange (ETDEWEB)

    Hair, Don

    1986-01-01

    In 1984, and under the auspices of the Northwest Power Planning Council, the Clear-water National Forest and the Bonneville Power Administration entered into a contractual agreement to improve anadromous fish habitat in Lolo Creek. This was to be the second and final year of instream enhancement work in Lolo Creek, a major tributary to the Clearwater River. The project was again entitled Lolo Creek Habitat Improvement (No.84-6) which was scheduled from April 1, 1984, through March 31, 1985. Project costs were not to exceed $39,109. The following report is a description of the project objectives, methodology, results, and conclusions of this year's work, based on the knowledge and experience gained through 2 years of enhancement work. The primary objective was to partially mitigate the juvenile and adult anadromous fish losses accrued through hydroelectric development in the Columbia and Snake River systems by enhancing the spawning and rearing habitats of selected Clearwater River tributaries for spring chinook salmon and summer steelhead trout. The enhancement was designed to ameliorate the ''limiting production factors'' by the in-stream placement of habitat structures that would positively alter the pool-riffle structure and increase the quality of over-winter habitat.

  9. Expandable/Foldable Structures for Habitat Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Folded Structures Company (FSC) has developed an innovative design approach for multi-laminate, primary and secondary structures for planetary habitats that...

  10. NMFS announces new habitat conservation policy

    Energy Technology Data Exchange (ETDEWEB)

    Nelson, R.W.

    A new habitat conservation policy to ensure that habitat is given greater attention in agency programs has been adopted by the National Marine Fisheries Service. Coastal and estuarine areas and their associated wetlands are important as spawning and nursery grounds for fishery resources. However, human population shifts to coastal areas and associated industrial and municipal expansion have accelerated competition for use of these habitats. These habitats have been substantially reduced and continue to suffer the effects of dredging, filling, coastal construction, energy development, pollution, waste disposal, and other human-related activities. This new policy will help protect certain marine mammals and endangered species.

  11. Habitat Ecology Visual Surveys of Demersal Fishes and Habitats off California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Since 1992, the Habitat Ecology team has been conducting fishery independent, visual surveys of demersal fishes and associated habitats in deep water (20 to 900...

  12. Habitat Evaluation and Monitoring in the Columbia River Basin, Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Everson, Larry B.; Campbell, Charles J.; Craven, Richard E.; Welsh, Thomas L.

    1986-12-01

    The law established the Northwest Power Planning Council to prepare and adopt a regional conservation and electric power plan, and a program to protect, mitigate, and enhance fish and wildlife. The objectives are the development of regional plans and programs related to energy conservation, renewable resources, other resources, and protecting mitigating, and enhancing fish and wildlife resources and to protect, mitigate, and enhance the fish and wildlife, including related spawning grounds and habitat, of the Columbia River and its tributaries. 4 refs.

  13. Habitat Evaluation Procedures (HEP) Report; Burlington Bottoms, Technical Report 1993-2003.

    Energy Technology Data Exchange (ETDEWEB)

    Beilke, Susan

    1993-08-01

    Burlington Bottoms, consisting of approximately 417 acres of riparian and wetland habitat, was purchased by the Bonneville Power Administration in November 1991. The site is located approximately 1/2 mile north of the Sauvie Island Bridge (T2N R1W Sections 20, 21), and is bound on the east side by Multnomah Channel and on the west side by the Burlington Northern Railroad right-of-way and U.S. Highway 30 (Figures 1 and 2). Wildlife habitat values resulting from the purchase of this site will contribute toward the goal of mitigating for habitat lost as outlined in the Columbia and Willamette River Basin's Fish and Wildlife Program and Amendments. Under this Program, mitigation goals were developed as a result of the loss of wildlife habitat due to the development and operation of Federal hydro-electric facilities in the Columbia and Willamette River Basins. In 1993, an interdisciplinary team was formed to develop and implement quantitative Habitat Evaluation Procedures (HEP) to document the value of various habitats at Burlington Bottoms. Results of the HEP will be used to: (1) determine the current status and habitat enhancement potential of the site consistent with wildlife mitigation goals and objectives; and (2) develop a management plan for the area. HEP participants included; Charlie Craig, BPA; Pat Wright, Larry Rasmussen, and Ron Garst, U. S. Fish and Wildlife Service; John Christy, The Nature Conservancy; and Doug Cottam, Sue Beilke, and Brad Rawls, Oregon Department of Fish and Wildlife.

  14. Habitat use by juvenile salmonids in Lake Ontario tributaries-species, age, diel and seasonal effects

    Science.gov (United States)

    Johnson, James H.; McKenna, James E.

    2017-01-01

    Understanding the habitat needs of fish and how these requirements may change seasonally over a 24-h period is important, especially for highly managed sport species. Consequently, we examined the diel and seasonal habitat use of four juvenile salmonid species in streams in the Lake Ontario watershed. For juvenile Atlantic salmon Salmo salarand juvenile rainbow trout Oncorhynchus mykiss, differences in day versus night habitat use were more profound than seasonal differences. Observed differences in day versus night habitat for all species and age classes were mainly due to the use of less object oriented cover at night and to a lesser extent to the use of slower velocities and smaller substrate at night. Seasonal differences in habitat use were also observed, likely due to increased fish size, and included movement to deeper and faster water and the use of larger substrate and more cover from summer to winter. Different habitat variables were important to individual species. Juvenile Atlantic salmon were associated with higher water velocities, juvenile rainbow trout with larger substrate and more cover, and subyearling Chinook salmon O. tshawytscha and subyearling coho salmon O. kisutch with small substrate and less cover. Our observations demonstrate that habitat partitioning occurs and likely reduces intraspecific and interspecific competition which may increase the potential production of all four species in sympatry. Consequently, these findings provide important information for resource managers charged with managing, protecting, and enhancing Great Lakes tributaries where all or some of these species occur.

  15. A randomized controlled dose-response pilot study of addition of hCG to recombinant FSH during controlled ovarian stimulation for in vitro fertilization

    DEFF Research Database (Denmark)

    Loft, A; Egeberg, Anne Noes; Smitz, J;

    2012-01-01

    Is it possible to define an optimal dose of hCG in combination with rFSH from the first day of stimulation in the GnRH agonist protocol applied to IVF? SUMMARY ANSWER: Supplementation with hCG from the first day of stimulation may increase the number of top-quality embryos per patient. Daily doses...... of hCG up to 150 IU are compatible with good live birth rates. A ceiling level of estradiol (E(2)) was reached with hCG doses above 100 IU/day. A positive dose-response was seen for pre-ovulatory progesterone, but concentrations remained below values for which an impairment of endometrial receptivity...... has been previously reported. We suggest a large clinical trial to be proceeded with a group given 100 IU hCG daily versus a control group. WHAT IS KNOWN AND WHAT THIS PAPER ADDS: Prospective multicentre studies have indicated increased live birth rates and increased number of top-quality embryos when...

  16. Mathematical Model for the Effect of Ghrelinon basal,GNRH Induced FSH and LH Secretion in Normal Women by using four Variate Weibull Distribution

    Directory of Open Access Journals (Sweden)

    S. Lakshmi

    2016-12-01

    Full Text Available In this paper, we introduce probability density function of four variate Weibull distributions. A multivariate survival function of Weibull Distribution is used for four variables. From the survival function, the probability density function and cumulative probability function are derived. Ghrelin may affect reproductive function in animals and humans.In the application part the experimental conditions of an acute injection of ghrelin (1g/kg to normal women, basal and GnRH-induced LH and FSH secretion were not affected and suggested that ghrelin does not play a major physiological role in gonadotrophin secretion in women.In the mathematical part, we have found that the Survival function of the curveis suddenly decreased in Mid-luteal phase compare with other phases. Pdf of the curve is suppressed in late follicular phase and it will be increased at the time of7min.Pdf for early follicular phase of control cycleis increased from 4 min .Also Pdf curve for early follicular phase with ghrelin administration and mid-luteal phase with ghrelin and GnRH are also increased at the time of 5 and 3 minutes respectively.

  17. Involvement of Ovarian Estradiol Biosynthesis and Pituitary FSH Expression in the Mechanism of Human Chorionic Gonadotropin Stimulation of Uterine Growth in Immature Female Rats

    Directory of Open Access Journals (Sweden)

    Sandrine Rafert

    2016-01-01

    Full Text Available In a previous work, we showed that human Chorionic Gonadotropin (hCG alone is able to stimulate uterine ponderal growth in immature female rats. In the present paper, we provide new information indicating that a single 100 ng hCG injection increased ovarian androgen availability and aromatase activity. These changes are consistent with an increase in ovarian estradiol secretion. Ovarian activin βA and activin βB subunit RNAs expression were also increased following hCG injection. Most interestingly, this treatment also led to an increase in FSHβ subunit mRNA expression in the pituitary, which might be due to hCG-stimulated ovarian activin secretion. We have not been able so far to follow the kinetics of the plasma concentrations of activin and FSH in hCG-treated animals compared to control animals. This has to be explored in the future to provide a more complete assessment of our model.

  18. In vitro seasonal variations of LH, FSH and prolactin secretion of the male rat are dependent on the maternal pineal gland.

    Science.gov (United States)

    Díaz, E; Vázquez, N; Fernández, C; Jiménez, V; Esquifino, A; Díaz, B

    2012-01-17

    The maternal pineal gland is involved in the seasonal rhythms entrainment. We evaluate the effect of maternal pinealectomy (PIN-X), also melatonin replacement (PIN-X+MEL) during pregnancy on "in vitro" gonadotropins and prolactin seasonal variations. Male offspring from control, PIN-X and PIN-X+MEL mother Wistar rats were studied at 31 and 60 days of age. In vitro LH release from controls was season-dependent during prepubertal and pubertal periods showing reduced values in winter. The mother pineal gland seems to be important in the entrainment of seasonal variations of in vitro pituitary LH release, since altered secretion showing very high values was observed in summer. Melatonin treatment to PIN-X mothers partially restored the LH response. The effect of pinealectomy upon LH secretion disappears at the pubertal phase. A different pattern was observed for FSH release, without seasonal variations at 31 or at 60 days of age in control offspring, but pinealectomy to mothers or melatonin treatment resulted in seasonal variations. Seasonal influence was also observed in the prolactin pituitary release of controls. PIN-X mother offspring showed delayed seasonal variations at 31 and 60 days of age. The effect of maternal melatonin treatment during pregnancy was observed up to 60 days of age.

  19. Data in support of FSH induction of IRS-2 in human granulosa cells: Mapping the transcription factor binding sites in human IRS-2 promoter

    Directory of Open Access Journals (Sweden)

    Surleen Kaur

    2016-03-01

    Full Text Available Insulin receptor substrate-2 (IRS-2 plays critical role in the regulation of various metabolic processes by insulin and IGF-1. The defects in its expression and/or function are linked to diseases like polycystic ovary syndrome (PCOS, insulin resistance and cancer. To predict the transcription factors (TFs responsible for the regulation of human IRS-2 gene expression, the transcription factor binding sites (TFBS and the corresponding TFs were investigated by analysis of IRS-2 promoter sequence using MatInspector Genomatix software (Cartharius et al., 2005 [1]. The ibid data is part of author׳s publication (Anjali et al., 2015 [2] that explains Follicle stimulating hormone (FSH mediated IRS-2 promoter activation in human granulosa cells and its importance in the pathophysiology of PCOS. Further analysis was carried out for binary interactions of TF regulatory genes in IRS-2 network using Cytoscape software tool and R-code. In this manuscript, we describe the methodology used for the identification of TFBSs in human IRS-2 promoter region and provide details on experimental procedures, analysis method, validation of data and also the raw files. The purpose of this article is to provide the data on all TFBSs in the promoter region of human IRS-2 gene as it has the potential for prediction of the regulation of IRS-2 gene in normal or diseased cells from patients with metabolic disorders and cancer.

  20. 超数排卵效果不同绵羊卵巢卵泡促卵泡素受体的分布%Distribution of FSH Receptor on the Ovaries Among Different Sheep Breeds by Different Efficiency of Superovulation

    Institute of Scientific and Technical Information of China (English)

    倪和民; 张鸿波; 刘云海; 芦天罡; 邓桂馨; 郭勇

    2011-01-01

    [目的]探讨在具有不同超数排卵效果的绵羊品种中,进行超排处理后其卵巢上促卵泡素受体(FSH receptor,FSHR)的分布情况,为进一步研究二者的相关性及据此改进超排效果奠定基础.[方法]根据超数排卵效果优劣依次选择3个中国地方品种的绵羊:小尾寒羊、白滩羊和乌珠穆沁羊.在常规超排最后一次注射FSH后的8、16和24 h分别各取3只绵羊的卵巢,并采用免疫组织化学法观察3种绵羊卵巢上FSHR的分布情况.[结果]FSHR主要分布于3个品种绵羊卵巢的颗粒细胞上;小尾寒羊最后一次注射FSH后8、16和24 h的各时期卵泡中FSHR的表达都显著高于另外两个品种绵羊(P白滩羊>乌珠穆沁羊,表明绵羊超数排卵效果与其卵巢上FSH受体分布存在一定联系.%[Objective]This study was aimed to investigate the distribution of FSHR (FSH receptor) on the ovaries among different local breeds of sheep by different efficiency of superovulation, and to do some preparations for further improving this ovine propagation technique.[Method]Three local ovine breeds were selected depending on their different own sensitivity range to superovulation by FSH as following: Small Tailed Han sheep, White Tan sheep, and Ujumqin sheep.At 8, 16, and 24 hour after the last injection with FSH, the ovine ovaries were collected and treated by immunohistochemical staining to observe the distribution of FSHR on their different ovarian follicles.[Result]The results showed that FSHR was expressed mainly on granulosa cells.At 8, 16,and 24 hour after the last injection with FSH, the FSHR levels on the ovarian follicles of Small Tailed Han sheep, were significantly higher than those of the other two breeds, while the FSHR levels in early and middle ovarian follicle of White Tan sheep, were also higher than Ujumqin sheep (P<0.05).At 8, 16, and 24 hour after the last injection of FSH, the FSHR levels in the late ovarian follicle of those three breeds were

  1. MEGAEPIFAUNA-HABITAT RELATIONSHIPS IN YAQUINA BAY, OR

    Science.gov (United States)

    Habitat-based ecological risk assessments rely, in part, on estimates of the ecological value of the habitats at risk. As part of a larger programmatic effort to estimate estuarine habitat values, we determined megaepifauna-habitat relationships for four major intertidal habitat...

  2. BENTHIC MACROFAUNA-HABITAT RELATIONSHIPS IN TWO PACIFIC NORTHWEST ESTUARIES

    Science.gov (United States)

    Habitat-based ecological risk assessments rely, in part, on estimates of the ecological value of the habitats at risk. As part of a larger programmatic effort to estimate estuarine habitat values, we determined benthic macrofauna-habitat relationships for 8 intertidal habitats i...

  3. Cloning and sequence analysis of follicle stimulating hormone (FSH) β subunit gene from swamp buffalo%沼泽型水牛促卵泡素基因β亚基的克隆与序列分析

    Institute of Scientific and Technical Information of China (English)

    陈宝剑; 蒋钦杨; 毛献宝; 郭亚芬; 韦英明

    2009-01-01

    根据GenBank公布的奶牛FSH β亚基的编码序列设计1对特异性引物,成功克隆出广西沼泽型水牛FSH β亚基cDNA,并将其序列提交到GenBank,获得接受号为EF710660.以DNA Star生物分析软件对其同源性进行分析,结果表明,广西沼泽型水牛与已公布的印度水牛(Bubalus bubalis)的FSH β亚基cDNA序列的同源性最高,达99%,与绵羊(Ovis aries)为96%、牛(Bovine)为95%、山羊(Capra hircus)为95%、梅花鹿(Cervus nippon)为95%.

  4. Subseafloor basalts as fungal habitats

    Science.gov (United States)

    Ivarsson, M.

    2012-09-01

    The oceanic crust is believed to host the largest potential habitat for microbial life on Earth, yet, still we lack substantial information about the abundance, diversity, and consequence of its biosphere. The last two decades have involved major research accomplishments within this field and a change in view of the ocean crust and its potential to harbour life. Here fossilised fungal colonies in subseafloor basalts are reported from three different seamounts in the Pacific Ocean. The fungal colonies consist of various characteristic structures interpreted as fungal hyphae, fruit bodies and spores. The fungal hyphae are well preserved with morphological characteristics such as hyphal walls, septa, thallic conidiogenesis, and hyphal tips with hyphal vesicles within. The fruit bodies consist of large (∼50-200 µm in diameter) body-like structures with a defined outer membrane and an interior filled with calcite. The fruit bodies have at some stage been emptied of their contents of spores and filled by carbonate-forming fluids. A few fruit bodies not filled by calcite and with spores still within support this interpretation. Spore-like structures (ranging from a few µm to ∼20 µm in diameter) are also observed outside of the fruit bodies and in some cases concentrated to openings in the membrane of the fruit bodies. The hyphae, fruit bodies and spores are all closely associated with a crust lining the vein walls that probably represent a mineralized biofilm. The results support a fungal presence in deep subseafloor basalts and indicate that such habitats were vital between ∼81 and 48 Ma.

  5. Habitat use by fishes in coral reefs, seagrass beds and mangrove habitats in the Philippines.

    Science.gov (United States)

    Honda, Kentaro; Nakamura, Yohei; Nakaoka, Masahiro; Uy, Wilfredo H; Fortes, Miguel D

    2013-01-01

    Understanding the interconnectivity of organisms among different habitats is a key requirement for generating effective management plans in coastal ecosystems, particularly when determining component habitat structures in marine protected areas. To elucidate the patterns of habitat use by fishes among coral, seagrass, and mangrove habitats, and between natural and transplanted mangroves, visual censuses were conducted semiannually at two sites in the Philippines during September and March 2010-2012. In total, 265 species and 15,930 individuals were recorded. Species richness and abundance of fishes were significantly higher in coral reefs (234 species, 12,306 individuals) than in seagrass (38 species, 1,198 individuals) and mangrove (47 species, 2,426 individuals) habitats. Similarity tests revealed a highly significant difference among the three habitats. Fishes exhibited two different strategies for habitat use, inhabiting either a single (85.6% of recorded species) or several habitats (14.4%). Some fish that utilized multiple habitats, such as Lutjanus monostigma and Parupeneus barberinus, showed possible ontogenetic habitat shifts from mangroves and/or seagrass habitats to coral reefs. Moreover, over 20% of commercial fish species used multiple habitats, highlighting the importance of including different habitat types within marine protected areas to achieve efficient and effective resource management. Neither species richness nor abundance of fishes significantly differed between natural and transplanted mangroves. In addition, 14 fish species were recorded in a 20-year-old transplanted mangrove area, and over 90% of these species used multiple habitats, further demonstrating the key role of transplanted mangroves as a reef fish habitat in this region.

  6. Habitat Impact on Ultraviolet Reflectance in Moths.

    Science.gov (United States)

    Zapletalová, L; Zapletal, M; Konvička, M

    2016-10-01

    A comparison of 95 species of Central European moths, representing 11 families and inhabiting various habitats, was carried out in order to detect the potential impact of biotope on the ultraviolet (UV) light reflectance of their wings. Based on digitized photographs taken under UV light conditions, a phylogeny-controlled redundancy analysis relating UV reflectance to preferred habitat type (xerophilous, mesophilous, and hygrophilous) and habitat openness (open, semiopen, and closed) was carried out. Species preferring hygrophilous habitats displayed significantly higher UV wing reflectance than species inhabiting xerothermic and mesic habitats, and this pattern remained significant even after controlling for phyletic relationships. In contrast, UV wing reflectance displayed no pattern related to habitat openness. Given the higher UV reflectance of water and humid surfaces, we interpret these results, which are based on the first comprehensive sampling of UV reflectance in Central European moths, in terms of predator avoidance under habitat-specific light conditions. We conclude that the moisture content of the environment may markedly contribute to the variation of appearance of moth wings for better imitation habitat characteristics and therefore to increase protection. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  7. Static Atmospheres in a Rotating Space Habitat.

    Science.gov (United States)

    McKinley, John M.

    1980-01-01

    Discusses O'Neill's proposal for the colonization of space as it offers new problems in pure physics. Addresses specifically the distribution of the atmosphere in O'Neill's habitat and whether there will be enough air at the axis of rotation to allow human-powered flight, with particular reference to the habitat's "artificial gravity."…

  8. Fern Habitats and Rare Ferns in Oklahoma

    Directory of Open Access Journals (Sweden)

    Bruce A. Smith

    2008-12-01

    Full Text Available This paper features some of the more common fern habitats in Oklahoma and provides information on four rare Oklahoma ferns from two fern families: Aspleniaceae and Pteridaceae. Surprisingly, ferns can be found in a variety of habitats across Oklahoma.

  9. Pollen and gene flow in fragmented habitats

    NARCIS (Netherlands)

    Kwak, Manja M.; Velterop, Odilia; van Andel, Jelte

    1998-01-01

    . Habitat fragmentation affects both plants and pollinators. Habitat fragmentation leads to changes in species richness, population number and size, density, and shape, thus to changes in the spatial arrangement of flowers. These changes influence the amount of food for flower-visiting insects and t

  10. Pollen and gene flow in fragmented habitats

    NARCIS (Netherlands)

    Kwak, Manja M.; Velterop, Odilia; van Andel, Jelte

    1998-01-01

    . Habitat fragmentation affects both plants and pollinators. Habitat fragmentation leads to changes in species richness, population number and size, density, and shape, thus to changes in the spatial arrangement of flowers. These changes influence the amount of food for flower-visiting insects and t

  11. Idaho Habitat Evaluation for Off-Site Mitigation Record : Annual Report 1987.

    Energy Technology Data Exchange (ETDEWEB)

    Petrosky, Charles E.; Holubetz, Terry B. (Idaho Dept. of Fish and Game, Boise, ID (USA)

    1988-04-01

    The Idaho Department of Fish and Game has been monitoring and evaluating existing and proposed habitat improvement projects for steelhead (Salmo gairdneri) and chinook salmon (Oncorhynchus tshawytscha) in the Clearwater and Salmon River drainages over the last four years. Projects included in the evaluation are funded by, or proposed for funding by, the Bonneville Power Administration (BPA) under the Northwest Power Planning Act as off-site mitigation for downstream hydropower development on the Snake and Columbia rivers. A mitigation record is being developed to use increased smolt production at full seeding as the best measure of benefit from a habitat enhancement project. Determination of full benefit from a project depends on presence of adequate numbers of fish to document actual increases in fish production. The depressed nature of upriver anadromous stocks have precluded attainment of full benefit of any habitat project in Idaho. Partial benefit will be credited to the mitigation record in the interim period of run restoration. According to the BPA Work Plan, project implementors have the primary responsibility for measuring physical habitat and estimating habitat change. To date, Idaho habitat projects have been implemented primarily by the US Forest Service (USFS). The Shoshone-Bannock Tribes (SBT) have sponsored three projects (Bear Valley Mine, Yankee Fork, and the proposed East Fork Salmon River projects). IDFG implemented two barrier-removal projects (Johnson Creek and Boulder Creek) that the USFS was unable to sponsor at that time. The role of IDFG in physical habitat monitoring is primarily to link habitat quality and habitat change to changes in actual, or potential, fish production. Individual papers were processed separately for the data base.

  12. Does habitat complexity influence fish recruitment?

    Directory of Open Access Journals (Sweden)

    A. CHEMINÉE

    2014-06-01

    Full Text Available Human activities facilitate coastal habitat transformation and homogenization. The spread of marine invasive species is one example. This in turn may influence fish recruitment and the subsequent replenishment of adult assemblages. We tested habitat complexity effect on fish (Teleostei recruitment by experimentally manipulating meadows of the habitat-forming invasive macroalga Caulerpa taxifolia (Chlorophyta. Among the fourteen fish species recorded during the experiment, only two labrids (Coris julis and Symphodus ocellatus settled in abundance among these meadows. Patterns in the abundance of these juveniles suggested that reduced tri-dimensional meadow complexity may reduce habitat quality and result in altered habitat choices and / or differential mortality of juveniles, therefore reducing fish recruitment and likely the abundance of adults.

  13. Habitat association in populations on landscapes with continuous-valued heterogeneous habitat quality.

    Science.gov (United States)

    Hiebeler, David E; Michaud, Isaac J; Wasserman, Ben A; Buchak, Timothy D

    2013-01-21

    We explore a spatially implicit patch-occupancy model of a population on a landscape with continuous-valued heterogeneous habitat quality, primarily considering the case where the habitat quality of a site affects the mortality rate but not the fecundity of individuals at that site. Two analytical approaches to the model are constructed, by summing over the sites in the landscape and by integrating over the range of habitat quality. We obtain results relating the equilibrium population density and all moments of the probability distribution of the habitat quality of occupied sites, and relating the probability distributions of total habitat quality and occupied habitat quality. Special cases are considered for landscapes where habitat quality has either a uniform or a linear probability density function. For these cases, we demonstrate habitat association, where the quality of occupied sites is higher than the overall mean quality of all sites; the discrepancy between the two is reduced at larger population densities. The variance of the quality of occupied sites may be greater or less than the overall variance of habitat quality, depending on the distribution of habitat quality across the landscape. Increasing the variance of habitat quality is also shown to increase the ability of a population to persist on a landscape.

  14. Effects of marine reserves versus nursery habitat availability on structure of reef fish communities.

    Science.gov (United States)

    Nagelkerken, Ivan; Grol, Monique G G; Mumby, Peter J

    2012-01-01

    No-take marine fishery reserves sustain commercial stocks by acting as buffers against overexploitation and enhancing fishery catches in adjacent areas through spillover. Likewise, nursery habitats such as mangroves enhance populations of some species in adjacent habitats. However, there is lack of understanding of the magnitude of stock enhancement and the effects on community structure when both protection from fishing and access to nurseries concurrently act as drivers of fish population dynamics. In this study we test the separate as well as interactive effects of marine reserves and nursery habitat proximity on structure and abundance of coral reef fish communities. Reserves had no effect on fish community composition, while proximity to nursery habitat only had a significant effect on community structure of species that use mangroves or seagrass beds as nurseries. In terms of reef fish biomass, proximity to nursery habitat by far outweighed (biomass 249% higher than that in areas with no nursery access) the effects of protection from fishing in reserves (biomass 21% lower than non-reserve areas) for small nursery fish (≤ 25 cm total length). For large-bodied individuals of nursery species (>25 cm total length), an additive effect was present for these two factors, although fish benefited more from fishing protection (203% higher biomass) than from proximity to nurseries (139% higher). The magnitude of elevated biomass for small fish on coral reefs due to proximity to nurseries was such that nursery habitats seem able to overrule the usually positive effects on fish biomass by reef reserves. As a result, conservation of nursery habitats gains importance and more consideration should be given to the ecological processes that occur along nursery-reef boundaries that connect neighboring ecosystems.

  15. Habitat Evaluation Procedures (HEP) Report : Rainwater Wildlife Area, 1998-2001 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Childs, Allen

    2004-01-01

    vegetation species, allowance of normative processes such as fire occurrence, and facilitating development of natural stable stream channels and associated floodplains. Implementation of habitat enhancement and restoration activities could generate an additional 1,850 habitat units in 10 years. Baseline and estimated future habitat units total 7,035.3 for the Rainwater Wildlife Area. Habitat protection, enhancement and restoration will require long-term commitments from managers to increase probabilities of success and meet the goals and objectives of the Northwest Power Planning Council's Fish and Wildlife Mitigation Program. Longer-term benefits of protection and enhancement activities include increases in native species diversity and plant community resiliency in all cover types. Watershed conditions, including floodplain/riparian, and instream habitat quality should improve as well providing multiple benefits for terrestrial and aquatic resources. While such benefits are not necessarily recognized by HEP models and reflected in the number of habitat units generated, they are consistent with the NPPC Fish and Wildlife Program.

  16. Habitat selection by mountain plovers in shortgrass steppe

    Science.gov (United States)

    Augustine, David J.

    2011-01-01

    Much of the breeding range for the mountain plover (Charadrius montanus) occurs in shortgrass steppe and mixed-grass prairie in the western Great Plains of North America. Studies of mountain plovers in shortgrass steppe during the 1970s and 1990s were focused in Weld County, Colorado, which was considered a key breeding area for the species. These studies, however, did not include habitats influenced by black-tailed prairie dogs (Cynomys ludovicianus) or prescribed fire. The role of these 2 rangeland disturbance processes has increased substantially over the past 15 years. During 2008–2009, I used radial distance point count surveys to estimate mountain plover densities early in the nesting season in 4 habitats on public lands in Weld County, Colorado. All 4 habitats were grazed by cattle during the growing season at moderate stocking rates but had different additional disturbances consisting of 1) dormant-season prescribed burns, 2) active black-tailed prairie dog colonies, 3) black-tailed prairie dog colonies affected by epizootic plague in the past 1–2 years, and 4) rangeland with no recent history of fire or prairie dogs. Mountain plover densities were similar on active black-tailed prairie dog colonies (x = 6.8 birds/km2, 95% CI = 4.3–10.6) and prescribed burns (x = 5.6 birds/km2, 95% CI = 3.5–9.1). In contrast, no plovers were detected at randomly selected rangeland sites grazed by cattle but lacking recent disturbance by prairie dogs or fire, even though survey effort was highest for this rangeland habitat. Mountain plover densities were intermediate (2.0 birds/km2, 95% CI = 0.8–5.0) on sites where black-tailed prairie dogs had recently been extirpated by plague. These findings suggest that prescribed burns and active black-tailed prairie dog colonies may enhance breeding habitat for mountain plovers in shortgrass steppe and illustrate the potential for suppressed or altered disturbance processes to influence habitat

  17. Dynamics of soil chemistry in different serpentine habitats from Serbia

    Directory of Open Access Journals (Sweden)

    Vicić Dražen D.

    2014-01-01

    Full Text Available To enhance understanding of edaphic conditions in serpentine habitats, a thorough investigation of chemical and mechanical properties of three soils from disjunct ultramafic outcrops in the central Balkans was undertaken. Soil from a nearby chemically-contrasting limestone habitat was also analyzed. Three plant species differently associated with serpentine (Halacsya sendtneri, Cheilanthes marantae, and Seseli rigidum were references for site and soil selection. Twenty elements were scanned for, and fourteen were measured in seven sequentially-extracted soil fractions. Quantified soil properties also included: pH, levels of free CaCO3, organic matter, P2O5, K2O, N, C, S, cation exchange capacity, total organic carbon, field capacity and soil mechanical composition. The usual harsh components for plant growth in serpentine soil such as elevated Mg:Ca ratio, high levels of Ni, Cr, or Co, were significantly lower in the available fractions. There was a significant positive correlation of organic matter and field capacity, with most available Ca (70-80% found in the mobile, rather than the organically-bound fraction. This showed that a more favorable Mg:Ca ratio is highly dependent upon a higher field capacity, which is also in accordance with a more developed vegetation. Increasing the availability of metals (Al, Ba, Ca, Cr, Cu, Mg, Ni, Zn in a more developed serpentine grassland and forest vegetation, occurred only simultaneously with decrease of the Mg:Ca ratio and rise in other factors of fertility (N, P, K. Progressive development of ecosystem complexity therefore raised the availability of metals, but also reduced harsh Mg:Ca ratio disproportion, boosted levels of nutrients and raised soil field capacity. Principal components analysis confirmed that the main differences among serpentine habitats lay primarily in factors of fertility. The common habitat which hosts all three reference species offers intermediate conditions in a plant habitat

  18. Upper Souris National Wildlife Refuge annual habitat work plan 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for Upper...

  19. Lostwood National Wildlife Refuge annual habitat work plan 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for...

  20. Rainwater Basin Wetland Management District annual habitat work plan 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for...

  1. Waubay National Wildlife Refuge Complex annual habitat work plan 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for...

  2. Huron Wetland Management District annual habitat work plan 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for Huron...

  3. Des Lacs National Wildlife Refuge annual habitat work plan 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for Des...

  4. Lee Metcalf National Wildlife Refuge 2005 annual habitat work plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for Lee...

  5. Bowdoin National Wildlife Refuge Complex 2005 annual habitat work plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for...

  6. Arapaho National Wildlife Refuge 2005 annual habitat work plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for...

  7. Boyer Chute National Wildlife Refuge 2005 annual habitat work plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for Boyer...

  8. Kulm Wetland Management District annual habitat work plan 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for Kulm...

  9. Tewaukon National Wildlife Refuge Complex annual habitat work plan 2005

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual habitat management plan outlines working habitat objectives for wetland habitats based on refuge purposes, professional judgment and experience for...

  10. Avian populations and habitat use in interior Alaska taiga

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Avian community structure, habitat occupancy levels, and species habitat use patterns were examined in the woody habitats of interior Alaska taiga. Some birds...

  11. Habitat Evaluation Procedures (HEP) Report, Pend Oreille Wetlands Wildlife I Project, Technical Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Holmes, Darren

    2003-05-01

    In 2002, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Pend Oreille Wetlands Wildlife Project, an acquisition completed by the Kalispel Tribe of Indians in 1992. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, and yellow warbler. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Pend Oreille Wetlands Wildlife Project provides a total of 936.76 Habitat Units (HUs) for the species evaluated. Open water habitat provides 71.92 HUs for Canada goose, mallard, and muskrat. Shoreline and island habitat provide 12.77 HUs fore Canada goose and mallard. Cattail hemi-marsh provides 308.42 HUs for Canada goose, mallard, and muskrat. Wet meadow provides 208.95 HUs for Canada goose and mallard. Scrub-shrub wetlands provide 14.43 HUs for yellow warbler, mallard, and white-tailed deer. Deciduous forested wetlands provide 148.62 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Grassland meadow provides 3.38 HUs for Canada goose. Conifer forest provides 160.44 HUs for bald eagle, black-capped chickadee, and white-tailed deer. The objective of using HEP at the Pend Oreille Wetlands Wildlife Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while

  12. Habitat Evaluation Procedures (HEP) Report; Iskuulpa Wildlife Mitigation and Watershed Project, Technical Report 1998-2003.

    Energy Technology Data Exchange (ETDEWEB)

    Quaempts, Eric

    2003-01-01

    , and the allowance of normative processes such as fire occurrence. Implementation of these alternatives could generate an estimated minimum of 393 enhancement credits in 10 years. Longer-term benefits of protection and enhancement activities include increases in native species diversity and structural complexity in all cover types. While such benefits are not readily recognized by HEP models and reflected in the number of habitat units generated, they also provide dual benefits for fisheries resources. Implementation of the alternatives will require long-term commitments from managers to increase probabilities of success and meet the goals and objectives of the Northwest Power Planning Council's Fish and Wildlife Mitigation Program.

  13. Effect of developmental low dose PBDE 47 exposure on thyroid hormone status and serum concentrations of FSH and inhibin B in male rats

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, A.J.M.; Kuriyama, S.N.; Akkoc, Z.; Talsness, C.E.; Chahoud, I. [Charite Univ. Medical School Berlin (Germany). Inst. of Clinical Pharmacology and Toxicology, Dept. Toxicology, Campus Benjamin Franklin

    2004-09-15

    Several persistent halogenated organic compounds such as PCBs, dioxins and more recently, polybrominated diphenyl ethers (PBDEs) have been shown to disrupt thyroid hormone homeostasis in experimental animals. Particular concern exists regarding exposure to these compounds during critical periods of development when thyroid hormones orchestrate the growth and differentiation of many organs including the brain and the testis. While the contamination levels of PCBs and other persistent organic pollutants have declined in the past years, increasing levels of PBDEs have been detected in environmental and human samples including human breast milk. PBDEs are produced in large quantities and used worldwide as flame retardants in electrical appliances, carpets and furniture upholstery. Similar to other halogenated environmental organic pollutants, PBDEs seem to present a wide range of toxic effects including reproductive, endocrine, neurobehavioral and hepatic toxicity. Recently, it has been demonstrated that in utero and pubertal exposures to DE-71 (a commercial mixture containing mostly tetra- and penta-bromodiphenyl ethers) significantly reduce thyroxine levels (T4) in rats. The present study has focused on the possible effects of the tetra- BDE congener 2,2'4,4'-tetrabromo diphenyl ether (PBDE 47) on thyroid hormone status and associated changes on FSH and inhibin B levels in the developing male rat. We administered a single dose to gravid dams on gestation day 6 of either 140 {mu}g/kg BW or 700 {mu}g/kg BW PBDE 47. These doses are pertinent to human exposure situation because a study by She et al.3 found a mean level of 33.3 {mu}g PBDE 47 /kg fat in human breast adipose tissue with a range from 7.01 to 196 {mu}g PBDE 47 /kg fat.

  14. Qualidade do corpo lúteo e do embrião de ovelhas Santa Inês superovuladas com FSH associado à rbST

    Directory of Open Access Journals (Sweden)

    I.M. Rocha do Nascimento

    2013-10-01

    Full Text Available Avaliou-se o efeito da somatotropina bovina recombinante (rbST sobre a qualidade do corpo lúteo e do embrião e sobre a concentração de progesterona (P4, em ovelhas da raça Santa Inês doadoras de embriões. Foram utilizadas 13 doadoras, distribuídas aleatoriamente em dois grupos, G1 (n=7 e G2 (n=6. Em ambos os grupos, as doadoras foram sincronizadas com implante vaginal por 14 dias, receberam 100µg de PGF2α (Ciosin® no sétimo dia e foram superovuladas, a partir do 12º dia, com 250UI de FSH (Folltopin-V® em seis doses decrescentes. No dia da retirada do implante, as doadoras do G2 receberam 125mg de rbST (Boostin® e foram cobertas por macho Santa Inês a cada 12 horas até o final do estro. No sexto dia após a primeira cobrição, foram avaliadas, por laparoscopia, a resposta superovulatória e a quantidade e a qualidade dos corpos lúteos. Após as avaliações, as colheitas embrionárias foram realizadas por laparotomia. A administração da rbST no protocolo de superovulação promoveu aumento significativo (P<0,05 no número de corpos lúteos do tipo I (9,00 x 5,28 e na qualidade embrionária (4,33 x 2,00.

  15. Fish habitat degradation in U.S. reservoirs

    Science.gov (United States)

    Miranda, Leandro E.; Spickard, M.; Dunn, T.; Webb, K.M.; Aycock, J.N.; Hunt, K.

    2010-01-01

    As the median age of the thousands of large reservoirs (> 200 ha) in the United States tops 50, many are showing various signs of fish habitat degradation. Our goal was to identify major factors degrading fish habitat in reservoirs across the country, and to explore regional degradation patterns. An online survey including 14 metrics was scored on a 0 (no degradation) to 5 (high degradation) point scale by 221 fisheries scientists (92% response rate) to describe degradation in 482 reservoirs randomly distributed throughout the continental United States. The highest scored sources of degradation were lack of aquatic macrophytes (41% of the reservoirs scored as 4–5), lack or loss of woody debris (35% scored 4–5), mistimed water level fluctuations (34% scored 4–5), and sedimentation (31% scored 4–5). Factor analysis identified five primary degradation factors that accounted for most of the variability in the 14 degradation metrics. The factors reflected siltation, structural habitat, eutrophication, water regime, and aquatic plants. Three degradation factors were driven principally by in-reservoir processes, whereas the other two were driven by inputs from the watershed. A comparison across U.S. regions indicated significant geographical differences in degradation relative to the factors emphasized by each region. Reservoirs sometimes have been dismissed as unnatural and disruptive, but they are a product of public policy, a critical feature of landscapes, and they cannot be overlooked if managers are to effectively conserve river systems. Protection and restoration of reservoir habitats may be enhanced with a broader perspective that includes watershed management, in addition to in reservoir activities.

  16. Home range dynamics, habitat selection, and survival of Greater Roadrunners

    Science.gov (United States)

    Kelley, S.W.; Ransom, D.; Butcher, J.A.; Schulz, G.G.; Surber, B.W.; Pinchak, W.E.; Santamaria, C.A.; Hurtado, L.A.

    2011-01-01

    Greater Roadrunners (Geococcyx californianus) are common, poorly studied birds of arid and semi-arid ecosystems in the southwestern United States. Conservation of this avian predator requires a detailed understanding of their movements and spatial requirements that is currently lacking. From 2006 to 2009, we quantified home-range and core area sizes and overlap, habitat selection, and survival of roadrunners (N= 14 males and 20 females) in north-central Texas using radio-telemetry and fixed kernel estimators. Median home-range and core-area sizes were 90.4 ha and 19.2 ha for males and 80.1 ha and 16.7 ha for females, respectively. The size of home range and core areas did not differ significantly by either sex or season. Our home range estimates were twice as large (x??= 108.9 ha) as earlier published estimates based on visual observations (x??= 28-50 ha). Mean percent overlap was 38.4% for home ranges and 13.7% for core areas. Male roadrunners preferred mesquite woodland and mesquite savanna cover types, and avoided the grass-forb cover type. Female roadrunners preferred mesquite savanna and riparian woodland cover types, and avoided grass-forb habitat. Kaplan-Meier annual survival probabilities for females (0.452 ?? 0.118[SE]) were twice that estimated for males (0.210 ?? 0.108), but this difference was not significant. Mortality rates of male roadrunners were higher than those of females during the spring when males call from elevated perches, court females, and chase competing males. Current land use practices that target woody-shrub removal to enhance livestock forage production could be detrimental to roadrunner populations by reducing availability of mesquite woodland and mesquite savanna habitat required for nesting and roosting and increasing the amount of grass-forb habitat that roadrunners avoid. ??2011 The Authors. Journal of Field Ornithology ??2011 Association of Field Ornithologists.

  17. Using EUNIS habitat classification for benthic mapping in European seas: present concerns and future needs.

    Science.gov (United States)

    Galparsoro, Ibon; Connor, David W; Borja, Angel; Aish, Annabelle; Amorim, Patricia; Bajjouk, Touria; Chambers, Caroline; Coggan, Roger; Dirberg, Guillaume; Ellwood, Helen; Evans, Douglas; Goodin, Kathleen L; Grehan, Anthony; Haldin, Jannica; Howell, Kerry; Jenkins, Chris; Michez, Noëmie; Mo, Giulia; Buhl-Mortensen, Pål; Pearce, Bryony; Populus, Jacques; Salomidi, Maria; Sánchez, Francisco; Serrano, Alberto; Shumchenia, Emily; Tempera, Fernando; Vasquez, Mickaël

    2012-12-01

    The EUNIS (European Union Nature Information System) habitat classification system aims to provide a common European reference set of habitat types within a hierarchical classification, and to cover all terrestrial, freshwater and marine habitats of Europe. The classification facilitates reporting of habitat data in a comparable manner, for use in nature conservation (e.g. inventories, monitoring and assessments), habitat mapping and environmental management. For the marine environment the importance of a univocal habitat classification system is confirmed by the fact that many European initiatives, aimed at marine mapping, assessment and reporting, are increasingly using EUNIS habitat categories and respective codes. For this reason substantial efforts have been made to include information on marine benthic habitats from different regions, aiming to provide a comprehensive geographical coverage of European seas. However, there still remain many concerns on its applicability as only a small fraction of Europe's seas are fully mapped and increasing knowledge and application raise further issues to be resolved. This paper presents an overview of the main discussion and conclusions of a workshop, organised by the MeshAtlantic project, focusing upon the experience in using the EUNIS habitats classification across different countries and seas, together with case studies. The aims of the meeting were to: (i) bring together scientists with experience in the use of the EUNIS marine classification and representatives from the European Environment Agency (EEA); (ii) agree on enhancements to EUNIS that ensure an improved representation of the European marine habitats; and (iii) establish practices that make marine habitat maps produced by scientists more consistent with the needs of managers and decision-makers. During the workshop challenges for the future development of EUNIS were identified, which have been classified into five categories: (1) structure and hierarchy; (2

  18. Modeling habitat dynamics accounting for possible misclassification

    Science.gov (United States)

    Veran, Sophie; Kleiner, Kevin J.; Choquet, Remi; Collazo, Jaime; Nichols, James D.

    2012-01-01

    Land cover data are widely used in ecology as land cover change is a major component of changes affecting ecological systems. Landscape change estimates are characterized by classification errors. Researchers have used error matrices to adjust estimates of areal extent, but estimation of land cover change is more difficult and more challenging, with error in classification being confused with change. We modeled land cover dynamics for a discrete set of habitat states. The approach accounts for state uncertainty to produce unbiased estimates of habitat transition probabilities using ground information to inform error rates. We consider the case when true and observed habitat states are available for the same geographic unit (pixel) and when true and observed states are obtained at one level of resolution, but transition probabilities estimated at a different level of resolution (aggregations of pixels). Simulation results showed a strong bias when estimating transition probabilities if misclassification was not accounted for. Scaling-up does not necessarily decrease the bias and can even increase it. Analyses of land cover data in the Southeast region of the USA showed that land change patterns appeared distorted if misclassification was not accounted for: rate of habitat turnover was artificially increased and habitat composition appeared more homogeneous. Not properly accounting for land cover misclassification can produce misleading inferences about habitat state and dynamics and also misleading predictions about species distributions based on habitat. Our models that explicitly account for state uncertainty should be useful in obtaining more accurate inferences about change from data that include errors.

  19. Does learning or instinct shape habitat selection?

    Directory of Open Access Journals (Sweden)

    Scott E Nielsen

    Full Text Available Habitat selection is an important behavioural process widely studied for its population-level effects. Models of habitat selection are, however, often fit without a mechanistic consideration. Here, we investigated whether patterns in habitat selection result from instinct or learning for a population of grizzly bears (Ursus arctos in Alberta, Canada. We found that habitat selection and relatedness were positively correlated in female bears during the fall season, with a trend in the spring, but not during any season for males. This suggests that habitat selection is a learned behaviour because males do not participate in parental care: a genetically predetermined behaviour (instinct would have resulted in habitat selection and relatedness correlations for both sexes. Geographic distance and home range overlap among animals did not alter correlations indicating that dispersal and spatial autocorrelation had little effect on the observed trends. These results suggest that habitat selection in grizzly bears are partly learned from their mothers, which could have implications for the translocation of wildlife to novel environments.

  20. Habitat selection by juvenile Mojave Desert tortoises

    Science.gov (United States)

    Todd, Brian D; Halstead, Brian J.; Chiquoine, Lindsay P.; Peaden, J. Mark; Buhlmann, Kurt A.; Tuberville, Tracey D.; Nafus, Melia G.

    2016-01-01

    Growing pressure to develop public lands for renewable energy production places several protected species at increased risk of habitat loss. One example is the Mojave desert tortoise (Gopherus agassizii), a species often at the center of conflicts over public land development. For this species and others on public lands, a better understanding of their habitat needs can help minimize negative impacts and facilitate protection or restoration of habitat. We used radio-telemetry to track 46 neonate and juvenile tortoises in the Eastern Mojave Desert, California, USA, to quantify habitat at tortoise locations and paired random points to assess habitat selection. Tortoise locations near burrows were more likely to be under canopy cover and had greater coverage of perennial plants (especially creosote [Larrea tridentata]), more coverage by washes, a greater number of small-mammal burrows, and fewer white bursage (Ambrosia dumosa) than random points. Active tortoise locations away from burrows were closer to washes and perennial plants than were random points. Our results can help planners locate juvenile tortoises and avoid impacts to habitat critical for this life stage. Additionally, our results provide targets for habitat protection and restoration and suggest that diverse and abundant small-mammal populations and the availability of creosote bush are vital for juvenile desert tortoises in the Eastern Mojave Desert.

  1. Does learning or instinct shape habitat selection?

    Science.gov (United States)

    Nielsen, Scott E; Shafer, Aaron B A; Boyce, Mark S; Stenhouse, Gordon B

    2013-01-01

    Habitat selection is an important behavioural process widely studied for its population-level effects. Models of habitat selection are, however, often fit without a mechanistic consideration. Here, we investigated whether patterns in habitat selection result from instinct or learning for a population of grizzly bears (Ursus arctos) in Alberta, Canada. We found that habitat selection and relatedness were positively correlated in female bears during the fall season, with a trend in the spring, but not during any season for males. This suggests that habitat selection is a learned behaviour because males do not participate in parental care: a genetically predetermined behaviour (instinct) would have resulted in habitat selection and relatedness correlations for both sexes. Geographic distance and home range overlap among animals did not alter correlations indicating that dispersal and spatial autocorrelation had little effect on the observed trends. These results suggest that habitat selection in grizzly bears are partly learned from their mothers, which could have implications for the translocation of wildlife to novel environments.

  2. The Gonadotropins FSH and LH and their use in Adult Women – an Overviewbr(Joint Statement by the German Society for Gynecological Endocrinology and Reproductive Medicine [DGGEF] and the German Professional Association of Gynecologists [BVF

    Directory of Open Access Journals (Sweden)

    Bachmann A

    2015-01-01

    Full Text Available The glycoprotein hormones LH and FSH are essential for reproduction. They consist of two polypeptide units. The alpha unit has 92 amino acids and is virtually identical for FSH, LH, thyroid-stimulating hormone (TSH and human chorionic gonadotropin (hCG. The secretion in the anterior pituitary gland underlies neuroendocrine control of the hypothalamic-pituitary-gonadal axis. Depending on the level of maturity of the follicle and on the phase of the menstrual cycle LH and FSH regulate the steroidogenesis in theka- and granulosacells of the ovary. The pregnancy hormone hCG is produced by Langerhans cells in the syncytiotrophoblast of the placenta. It is very similar to LH in structure and binds to the LH receptor, but with a half-life of 36 h has a longer effect in duration. hCG administration can replace the LH peak in the middle of the ovarian cycle and helps to improve luteal function. However, it cannot prevent regression of the corpus luteum. For more than 30 years gonadotropins have been crucial elements of pharmacological ovarian stimulation in assisted reproductive techniques (ART in primary and secondary infertility and the treatment of hypogonadotropic hypogonadism. First menotropins have been extracted from the urine of postmenopausal women. The application of recombinant DNA technology has resulted in the development of recombinant FSH. Further aims have been to avoid daily injections by modifying the FSH molecule.br Compounds available at present:br – Highly purified human-derived follicle-stimulating hormone: Bravelle® (Ferringbr – Recombinant-DNA products:br -- Follitropin alpha: Gonal-F® (Merck-Seronobr -- rec. FSH: Follitropin beta: Puregon® (MSDbr -- long acting rec. FSH: Corifollitropin alpha: Elonva® (MSDbr -- rec. hLH: Lutropin alpha: Luveris® (Merck Seronobr – Compounds with more than one active agent:br -- human urinary-derived preparations: hMG HP: FSH,LH,hCG = 75 IU LH: Menogon HP® (Ferringbr -- recombinant fixed

  3. Habitat Evaluation Procedures (HEP) Report; Big Island - The McKenzie River, Technical Report 1998-2001.

    Energy Technology Data Exchange (ETDEWEB)

    Sieglitz, Greg

    2001-03-01

    The Big Island site is located in the McKenzie River flood plain, containing remnant habitats of what was once more common in this area. A diverse array of flora and fauna, representing significant wildlife habitats, is present on the site. Stands of undisturbed forested wetlands, along with riparian shrub habitats and numerous streams and ponds, support a diversity of wildlife species, including neotropical migratory songbirds, raptors, mammals, reptiles, and amphibians (including two State-listed Sensitive Critical species). The project is located in eastern Springfield, Oregon (Figure 1). The project area encompasses 187 acres under several ownerships in Section 27 of Township 17S, Range 2W. Despite some invasion of non-native species, the site contains large areas of relatively undisturbed wildlife habitat. Over several site visits, a variety of wildlife and signs of wildlife were observed, including an active great blue heron rookery, red-Legged frog egg masses, signs of beaver, and a bald eagle, Wildlife habitat values resulting from the purchase of this site will contribute toward the goal of mitigating for habitat lost as outlined in the Bonneville Power Administration's (BPA) Mitigation and Enhancement Plan for the Willamette River Basin. Under this Plan, mitigation goals and objectives were developed as a result of the loss of wildlife habitat due to the construction of Federal hydroelectric facilities in the Willamette River Basin. Results of the Habitat Evaluation Procedures (HEP) will be used to: (1) determine the current habitat status of the study area and habitat enhancement potential of the site consistent with wildlife mitigation goals and objectives; and (2) develop a management plan for the area.

  4. Clinical research on idiopathic disease in the treatment with pure urinary FSH%尿促卵泡素用于特发性少弱精子症的疗效观察

    Institute of Scientific and Technical Information of China (English)

    谭强; 王希斌

    2012-01-01

    . Various clinical indicators of two groups followed up were comparied. Results After treatment, the sperm density, sperm motility, sperm survival rate of patients in the control group increased significantly, while the semen volume did not significantly increase and abnormal sperm rate was not obvious. After treatment, the semen volume, sperm density, sperm motility, sperm survival rate of patients in the observation group increased significantly, while abnormal sperm rate decreased obviously. After treatment, the semen volume, sperm density, sperm motility, sperm survival rate of patients in the observation group were significantly higher than those in the control group, while abnormal sperm rate of patients in the observation group was lower than that in the control group. After treatment, total effective rate and pregnancy rate in the observation group were significantly higher than those in the control group. Adverse reaction rate in the observation group was significantly higher than that in the control group. The differences were statistically significant (P < 0.05). Conclusion Using pure urinary FSH in the treatment with male idiopathic oligospermia and asthenospermia patients not only can improve the semen volume and improve sperm density, but also can enhance patients' sperm activity and improve the sperm survival rate. That can improve the spouses of patients with pregnancy incidence rate, which is a safe and effective method of treatment.

  5. Survival in patchy landscapes: the interplay between dispersal, habitat loss and fragmentation.

    Science.gov (United States)

    Niebuhr, Bernardo B S; Wosniack, Marina E; Santos, Marcos C; Raposo, Ernesto P; Viswanathan, Gandhimohan M; da Luz, Marcos G E; Pie, Marcio R

    2015-07-07

    Habitat loss and fragmentation are important factors determining animal population dynamics and spatial distribution. Such landscape changes can lead to the deleterious impact of a significant drop in the number of species, caused by critically reduced survival rates for organisms. In order to obtain a deeper understanding of the threeway interplay between habitat loss, fragmentation and survival rates, we propose here a spatially explicit multi-scaled movement model of individuals that search for habitat. By considering basic ecological processes, such as predation, starvation (outside the habitat area), and competition, together with dispersal movement as a link among habitat areas, we show that a higher survival rate is achieved in instances with a lower number of patches of larger areas. Our results demonstrate how movement may counterbalance the effects of habitat loss and fragmentation in altered landscapes. In particular, they have important implications for conservation planning and ecosystem management, including the design of specific features of conservation areas in order to enhance landscape connectivity and population viability.

  6. Access to Science and Literacy through Inquiry and School Yard Habitats

    Science.gov (United States)

    Cox-Petersen, Anne; Spencer, Brenda

    2006-01-01

    In this article, the authors describe an integrated science and literacy instructional model in which students build background knowledge by engaging in free-choice learning options during an investigation of school yard habitats. Students interact with their peers while inquiring, discussing findings, and using print resources to enhance learning.

  7. 75 FR 38768 - Ashley National Forest, UT, High Uintas Wilderness-Colorado River Cutthroat Trout Habitat...

    Science.gov (United States)

    2010-07-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Ashley National Forest, UT, High Uintas Wilderness--Colorado River Cutthroat Trout Habitat Enhancement AGENCY: Forest Service, USDA. ACTION: Notice of intent to prepare an environmental...

  8. Gulf-Wide Information System, Environmental Sensitivity Index Habitats Database, Geographic NAD83, LDWF (2001) [esi_habitats_LDWF_2001

    Data.gov (United States)

    Louisiana Geographic Information Center — This data set contains sensitive biological resource data for coastal habitats in Louisiana. Vector polygons represent various habitats, including marsh types, other...

  9. Landsat-based Earth Observations and Crowd-sourced Data Provide Near Real-time Monitoring of Chimpanzee Habitat

    Science.gov (United States)

    Nackoney, J.; Pintea, L.; Jantz, S.; Hansen, M.

    2015-12-01

    The endangered chimpanzee (Pan troglodytes) is threatened by habitat loss from resource extraction and land conversion, as well as hunting, disease and the illegal pet trade. It has been estimated that more than 70% of chimpanzee's tropical forest habitats in Africa are now threatened by land use change. Recent developments in remote sensing and cloud computing enable the use of satellite observations to provide a synoptic view of chimpanzee habitats at finer spatial and temporal resolutions that are locally relevant and consistent across the entire species' range. We present a practical Decision Support System to be used by the Jane Goodall Institute and partners to annually monitor and forecast chimpanzee habitat health in Africa. The system integrates Earth observations from 30-meter resolution Landsat data with a species-specific habitat model and a model forecasting future land use change, enhanced by crowd-sourced field data collected by local communities and rangers using the Open Data Kit app and Android mobile smartphones and tablets. While coarser-scale and static chimpanzee habitat models have been previously developed, this project is the first to develop a dynamic monitoring system updated annually via Earth observations data that will systematically monitor threats and changes in habitat over time. Since the chimpanzee is an important keystone, flagship and umbrella species, an annual chimpanzee habitat health index would support conservation goals of other species within its large 2.5 million sq. km range and could be an important indicator of overall ecosystem health of tropical forests in Africa.

  10. Latitudinal gradients in ecosystem engineering by oysters vary across habitats.

    Science.gov (United States)

    McAfee, Dominic; Cole, Victoria J; Bishop, Melanie J

    2016-04-01

    Ecological theory predicts that positive interactions among organisms will increase across gradients of increasing abiotic stress or consumer pressure. This theory has been supported by empirical studies examining the magnitude of ecosystem engineering across environmental gradients and between habitat settings at local scale. Predictions that habitat setting, by modifying both biotic and abiotic factors, will determine large-scale gradients in ecosystem engineering have not been tested, however. A combination of manipulative experiments and field surveys assessed whether along the east Australian coastline: (1) facilitation of invertebrates by the oyster Saccostrea glomerata increased across a latitudinal gradient in temperature; and (2) the magnitude of this effect varied between intertidal rocky shores and mangrove forests. It was expected that on rocky shores, where oysters are the primary ecosystem engineer, they would play a greater role in ameliorating latitudinal gradients in temperature than in mangroves, where they are a secondary ecosystem engineer living under the mangrove canopy. On rocky shores, the enhancement of invertebrate abundance in oysters as compared to bare microhabitat decreased with latitude, as the maximum temperatures experienced by intertidal organisms diminished. By contrast, in mangrove forests, where the mangrove canopy resulted in maximum temperatures that were cooler and of greater humidity than on rocky shores, we found no evidence of latitudinal gradients of oyster effects on invertebrate abundance. Contrary to predictions, the magnitude by which oysters enhanced biodiversity was in many instances similar between mangroves and rocky shores. Whether habitat-context modifies patterns of spatial variation in the effects of ecosystem engineers on community structure will depend, in part, on the extent to which the environmental amelioration provided by an ecosystem engineer replicates that of other co-occurring ecosystem engineers.

  11. Natural Propagation and Habitat Improvement, Volume 1, Oregon, 1985 Annual and Final Reports.

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, Ken

    1986-10-01

    The Hot Springs Fork of the Collawash River is a major sub-drainage in the Clackamas River drainage. Emphasis species for natural production are spring chinook, coho salmon, and winter steelhead. Increased natural production appears limited by a lack of quality rearing habitat. Habitat complexity over approximately 70% of accessible area to anadromous fish has been reduced over the last 40 years by numerous factors. Natural passage barriers limit anadromous fish access to over 7 miles of high quality habitat. In the first year of a multi-year effort to improve fish habitat in the Hot Springs Fork drainage, passage enhancement on two tributaries and channel rehabilitation on one of those tributaries was completed. Three waterfalls on Nohorn Creek were evaluated and passage improved on the uppermost waterfall to provide steelhead full access to 2.4 miles of good quality habitat. The work was completed in October 1985 and involved blasting three jump pools and two holding pools into the waterfall. On Pansy Creek, four potential passage barriers were evaluated and passage improvement work conducted on two logjams and one waterfall. Minor modifications were made to a waterfall to increase flow into a side channel which allows passage around the waterfall. Channel rehabilitation efforts on Pansy Creek (RM 0.0 to 0.3) to increase low flow pool rearing habitat and spawning habitat including blasting five pools into areas of bedrock substrate and using a track-mounted backhoe to construct instream structures. On site materials were used to construct three log sills, three boulder berms, a boulder flow deflector, and five log and boulder structures. Also, an alcove was excavated to provide overwinter rearing habitat. Pre-project monitoring consisting of physical and biological data collection was completed in the project area.

  12. Anthropogenic Habitats Facilitate Dispersal of an Early Successional Obligate: Implications for Restoration of an Endangered Ecosystem.

    Directory of Open Access Journals (Sweden)

    Katrina E Amaral

    Full Text Available Landscape modification and habitat fragmentation disrupt the connectivity of natural landscapes, with major consequences for biodiversity. Species that require patchily distributed habitats, such as those that specialize on early successional ecosystems, must disperse through a landscape matrix with unsuitable habitat types. We evaluated landscape effects on dispersal of an early successional obligate, the New England cottontail (Sylvilagus transitionalis. Using a landscape genetics approach, we identified barriers and facilitators of gene flow and connectivity corridors for a population of cottontails in the northeastern United States. We modeled dispersal in relation to landscape structure and composition and tested hypotheses about the influence of habitat fragmentation on gene flow. Anthropogenic and natural shrubland habitats facilitated gene flow, while the remainder of the matrix, particularly development and forest, impeded gene flow. The relative influence of matrix habitats differed between study areas in relation to a fragmentation gradient. Barrier features had higher explanatory power in the more fragmented site, while facilitating features were important in the less fragmented site. Landscape models that included a simultaneous barrier and facilitating effect of roads had higher explanatory power than models that considered either effect separately, supporting the hypothesis that roads act as both barriers and facilitators at all spatial scales. The inclusion of LiDAR-identified shrubland habitat improved the fit of our facilitator models. Corridor analyses using circuit and least cost path approaches revealed the importance of anthropogenic, linear features for restoring connectivity between the study areas. In fragmented landscapes, human-modified habitats may enhance functional connectivity by providing suitable dispersal conduits for early successional specialists.

  13. Anthropogenic Habitats Facilitate Dispersal of an Early Successional Obligate: Implications for Restoration of an Endangered Ecosystem.

    Science.gov (United States)

    Amaral, Katrina E; Palace, Michael; O'Brien, Kathleen M; Fenderson, Lindsey E; Kovach, Adrienne I

    2016-01-01

    Landscape modification and habitat fragmentation disrupt the connectivity of natural landscapes, with major consequences for biodiversity. Species that require patchily distributed habitats, such as those that specialize on early successional ecosystems, must disperse through a landscape matrix with unsuitable habitat types. We evaluated landscape effects on dispersal of an early successional obligate, the New England cottontail (Sylvilagus transitionalis). Using a landscape genetics approach, we identified barriers and facilitators of gene flow and connectivity corridors for a population of cottontails in the northeastern United States. We modeled dispersal in relation to landscape structure and composition and tested hypotheses about the influence of habitat fragmentation on gene flow. Anthropogenic and natural shrubland habitats facilitated gene flow, while the remainder of the matrix, particularly development and forest, impeded gene flow. The relative influence of matrix habitats differed between study areas in relation to a fragmentation gradient. Barrier features had higher explanatory power in the more fragmented site, while facilitating features were important in the less fragmented site. Landscape models that included a simultaneous barrier and facilitating effect of roads had higher explanatory power than models that considered either effect separately, supporting the hypothesis that roads act as both barriers and facilitators at all spatial scales. The inclusion of LiDAR-identified shrubland habitat improved the fit of our facilitator models. Corridor analyses using circuit and least cost path approaches revealed the importance of anthropogenic, linear features for restoring connectivity between the study areas. In fragmented landscapes, human-modified habitats may enhance functional connectivity by providing suitable dispersal conduits for early successional specialists.

  14. Waterfowl Breeding Population and Habitat Survey

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Waterfowl Breeding Population and Habitat Survey was initiated experimentally in 1947 and became operational in 1955. It is conducted cooperatively by the U.S....

  15. Habitat Mapping Cruise (HB0805, EK60)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Objectives are to: 1) perform multibeam mapping of transitional and deepwater habitats in Hudson Canyon (off New Jersey) with the National Institute of Undersea...

  16. Final Critical Habitat for the Koster's Springsnail

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for Koster's springsnail occur. The geographic extent includes Chaves County, New Mexico.

  17. Concept Plan for Waterfowl Habitat Protection

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The San Francisco Bay Area is one of 34 Waterfowl Habitat Areas of Major Concern (#27) in Canada and the United States identified in the North American Waterfowl...

  18. Riparian Habitats - Sierra Nevada Foothill [ds304

    Data.gov (United States)

    California Department of Resources — These data are habitat polygons within a 200-m radius around point locations where wildlife surveys were conducted along 19 randomly selected watercourses in the...

  19. Habitat Testbed (HaT) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Goals of the DSH Testbed include:Function as a habitat systems integrator and technology pull across many domainsDevelop and integrate software-based models of...

  20. Habitat--Offshore of Salt Point, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for the habitat map of the seafloor of the Offshore of Salt Point map area, California. The vector data file is included in...