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Sample records for fluorescence plate reader

  1. Fluorescence lifetime plate reader: resolution and precision meet high-throughput.

    Science.gov (United States)

    Petersen, Karl J; Peterson, Kurt C; Muretta, Joseph M; Higgins, Sutton E; Gillispie, Gregory D; Thomas, David D

    2014-11-01

    We describe a nanosecond time-resolved fluorescence spectrometer that acquires fluorescence decay waveforms from each well of a 384-well microplate in 3 min with signal-to-noise exceeding 400 using direct waveform recording. The instrument combines high-energy pulsed laser sources (5-10 kHz repetition rate) with a photomultiplier and high-speed digitizer (1 GHz) to record a fluorescence decay waveform after each pulse. Waveforms acquired from rhodamine or 5-((2-aminoethyl)amino) naphthalene-1-sulfonic acid dyes in a 384-well plate gave lifetime measurements 5- to 25-fold more precise than the simultaneous intensity measurements. Lifetimes as short as 0.04 ns were acquired by interleaving with an effective sample rate of 5 GHz. Lifetime measurements resolved mixtures of single-exponential dyes with better than 1% accuracy. The fluorescence lifetime plate reader enables multiple-well fluorescence lifetime measurements with an acquisition time of 0.5 s per well, suitable for high-throughput fluorescence lifetime screening applications.

  2. Establishing a cellular FRET-based fluorescence plate reader assay to monitor proNGF-induced cross-linking of sortilin and the neurotrophin receptor p75(NTR)

    DEFF Research Database (Denmark)

    Skeldal, Sune; Kjaergaard, Maj M; Alwasel, Saleh

    2015-01-01

    the vps10p domain receptor sortilin and the neurotrophin receptor p75(NTR). However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay...... to monitor the interaction between fluorescently tagged sortilin and p75(NTR) in live cells. The method is based on a standard fluorescent plate reader found in many biochemical laboratories and the results are evaluated using a microscopy-based quantified sensitized acceptor emission FRET approach making...

  3. Comparison of plate reader-based methods with fluorescence microscopy for measurements of intracellular calcium levels for the assessment of in vitro neurotoxicity.

    Science.gov (United States)

    Meijer, Marieke; Hendriks, Hester S; Heusinkveld, Harm J; Langeveld, Wendy T; Westerink, Remco H S

    2014-12-01

    The intracellular calcium concentration ([Ca(2+)]i) is an important readout for in vitro neurotoxicity since calcium is critically involved in many essential neurobiological processes, including neurotransmission, neurodegeneration and neurodevelopment. [Ca(2+)]i is often measured with considerable throughput at the level of cell populations with plate reader-based assays or with lower throughput at the level of individual cells with fluorescence microscopy. However, these methodologies yield different quantitative and qualitative results. In recent years, we demonstrated that the resolution and sensitivity of fluorescence microscopy is superior compared to plate reader-based assays. However, it is currently unclear if the use of plate reader-based assays results in more 'false negatives' or 'false positives' in neurotoxicity screening studies. In the present study, we therefore compared a plate reader-based assay with fluorescence microscopy using a small test set of environmental pollutants consisting of dieldrin, lindane, polychlorinated biphenyl 53 (PCB53) and tetrabromobisphenol-A (TBBPA). Using single-cell fluorescence microscopy, we demonstrate that all test chemicals reduce the depolarization-evoked increase in [Ca(2+)]i, whereas lindane, PCB53 and TBBPA also increase basal [Ca(2+)]i, though via different mechanisms. Importantly, none of these effects were confirmed with the plate reader-based assay. We therefore conclude that standard plate reader-based methods are not sufficiently sensitive and reliable to measure the highly dynamic and transient changes in [Ca(2+)]i that occur during chemical exposure. Copyright © 2014 Elsevier Inc. All rights reserved.

  4. Establishing a cellular FRET-based fluorescence plate reader assay to monitor proNGF-induced cross-linking of sortilin and the neurotrophin receptor p75(NTR).

    Science.gov (United States)

    Skeldal, Sune; Kjaergaard, Maj M; Alwasel, Saleh; Nyengaard, Jens R

    2015-01-01

    Whereas the proform of the nerve growth factor (proNGF) is crucial for eliminating superfluous cells during neuronal development it also promotes apoptosis following brain trauma and neuronal injury. The apoptotic signal is elicited upon formation of a trimeric receptor complex also containing the vps10p domain receptor sortilin and the neurotrophin receptor p75(NTR). However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75(NTR) in live cells. The method is based on a standard fluorescent plate reader found in many biochemical laboratories and the results are evaluated using a microscopy-based quantified sensitized acceptor emission FRET approach making use of a pair of FRET standard constructs. As a result, the effect of proNGF on the interaction between sortilin and p75(NTR) can be evaluated in live cells allowing for screening and selection of therapeutic compounds interfering with proNGF-induced cell death.

  5. Solid-phase plate-reader quantification of specific PCR products by measurement of band-specific ethidium bromide fluorescence.

    Science.gov (United States)

    McCarthy, Michael T; O'Callaghan, Christopher A

    2014-02-15

    Real-time PCR is widely employed to quantify PCR products across a range of applications. However, accurate real-time PCR is not always technically feasible, and alternative methods for PCR product quantification can be expensive and time consuming to validate. We have developed an inexpensive, rapid, and immediately accessible protocol to quantify PCR products, by measuring ethidium bromide fluorescence of PCR products excised from agarose gels. This protocol has relevance to a broad range of methods in molecular biology where quantification of PCR products is necessary.

  6. PC-based car license plate reader

    Science.gov (United States)

    Hwang, Chung-Mu; Shu, Shyh-Yeong; Chen, Wen-Yu; Chen, Yie-Wern; Wen, Kuang-Pu

    1992-11-01

    A car license plate reader (CLPR) using fuzzy inference and neural network algorithm has been developed in Industrial Technology Research Institute (ITRI) and installed in highway toll stations to identify stolen cars. It takes an average of 0.7 seconds to recognize a car license plate by using a PC with 80486-50 CPU. The recognition rate of the system is about 97%. The techniques of CLPR include vehicle sensing, image grab control, optic pre- processing, lighting, and optic character recognition (OCR). The CLPR can be used in vehicle flow statistics, the checking of stolen vehicles, automatic charging systems in parking lots or garage management, and so on.

  7. Spectral Unmixing Plate Reader: High-Throughput, High-Precision FRET Assays in Living Cells.

    Science.gov (United States)

    Schaaf, Tory M; Peterson, Kurt C; Grant, Benjamin D; Thomas, David D; Gillispie, Gregory D

    2017-03-01

    We have developed a microplate reader that records a complete high-quality fluorescence emission spectrum on a well-by-well basis under true high-throughput screening (HTS) conditions. The read time for an entire 384-well plate is less than 3 min. This instrument is particularly well suited for assays based on fluorescence resonance energy transfer (FRET). Intramolecular protein biosensors with genetically encoded green fluorescent protein (GFP) donor and red fluorescent protein (RFP) acceptor tags at positions sensitive to structural changes were stably expressed and studied in living HEK cells. Accurate quantitation of FRET was achieved by decomposing each observed spectrum into a linear combination of four component (basis) spectra (GFP emission, RFP emission, water Raman, and cell autofluorescence). Excitation and detection are both conducted from the top, allowing for thermoelectric control of the sample temperature from below. This spectral unmixing plate reader (SUPR) delivers an unprecedented combination of speed, precision, and accuracy for studying ensemble-averaged FRET in living cells. It complements our previously reported fluorescence lifetime plate reader, which offers the feature of resolving multiple FRET populations within the ensemble. The combination of these two direct waveform-recording technologies greatly enhances the precision and information content for HTS in drug discovery.

  8. 77 FR 58580 - Interview Room Recording System Standard and License Plate Reader Standard Workshops

    Science.gov (United States)

    2012-09-21

    ... of Justice Programs Interview Room Recording System Standard and License Plate Reader Standard... System Standard and License Plate Reader Standard Workshops. SUMMARY: The National Institute of Justice... development of NIJ performance standards for Interview Room Recording Systems and License Plate Readers used...

  9. 78 FR 59065 - Interview Room Recording System Standard and License Plate Reader Standard Workshops

    Science.gov (United States)

    2013-09-25

    ... of Justice Programs Interview Room Recording System Standard and License Plate Reader Standard... Recording System Standard and License Plate Reader Standard Workshops. SUMMARY: The National Institute of... Plate Readers used by criminal justice agencies. Sessions are intended to inform manufacturers, test...

  10. Caveats and limitations of plate reader-based high-throughput kinetic measurements of intracellular calcium levels.

    Science.gov (United States)

    Heusinkveld, Harm J; Westerink, Remco H S

    2011-08-15

    Calcium plays a crucial role in virtually all cellular processes, including neurotransmission. The intracellular Ca(2+) concentration ([Ca(2+)](i)) is therefore an important readout in neurotoxicological and neuropharmacological studies. Consequently, there is an increasing demand for high-throughput measurements of [Ca(2+)](i), e.g. using multi-well microplate readers, in hazard characterization, human risk assessment and drug development. However, changes in [Ca(2+)](i) are highly dynamic, thereby creating challenges for high-throughput measurements. Nonetheless, several protocols are now available for real-time kinetic measurement of [Ca(2+)](i) in plate reader systems, though the results of such plate reader-based measurements have been questioned. In view of the increasing use of plate reader systems for measurements of [Ca(2+)](i) a careful evaluation of current technologies is warranted. We therefore performed an extensive set of experiments, using two cell lines (PC12 and B35) and two fluorescent calcium-sensitive dyes (Fluo-4 and Fura-2), for comparison of a linear plate reader system with single cell fluorescence microscopy. Our data demonstrate that the use of plate reader systems for high-throughput real-time kinetic measurements of [Ca(2+)](i) is associated with many pitfalls and limitations, including erroneous sustained increases in fluorescence, limited sensitivity and lack of single cell resolution. Additionally, our data demonstrate that probenecid, which is often used to prevent dye leakage, effectively inhibits the depolarization-evoked increase in [Ca(2+)](i). Overall, the data indicate that the use of current plate reader-based strategies for high-throughput real-time kinetic measurements of [Ca(2+)](i) is associated with caveats and limitations that require further investigation. Copyright © 2011 Elsevier Inc. All rights reserved.

  11. Reproducibility of High-Throughput Plate-Reader Experiments in Synthetic Biology.

    Science.gov (United States)

    Chavez, Michael; Ho, Jonathan; Tan, Cheemeng

    2017-02-17

    Plate-reader assays are commonly conducted to quantify the performance of synthetic biological systems. However, on the basis of a survey of 100 publications, we find that most publications do not report critical experimental settings of plate reader assays, suggesting widespread issues in their reproducibility. Specifically, critical plate reader settings, including shaking time and covering method, either vary between laboratories or are not reported by the publications. Here, we demonstrate that the settings of plate reader assays have a significant impact on bacterial growth, recombinant gene expression, and biofilm formation. Furthermore, we show that the plate reader settings affect the apparent activity, sensitivity, and chemical kinetics of synthetic constructs, as well as alter the apparent effectiveness of antibiotics. Our results suggest the critical need for consistent reporting of plate reader protocols to ensure the reproducibility of the protocols. In addition, our work provides data for the setup of plate reader protocols in synthetic biology experiments.

  12. 75 FR 75186 - License Plate Reader Standard Special Technical Committee Request for Proposals for Certification...

    Science.gov (United States)

    2010-12-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF JUSTICE Office of Justice Programs License Plate Reader Standard Special Technical Committee Request for Proposals... the process of developing a new License Plate Reader Standard and corresponding certification program...

  13. Laser-induced fluorescence reader with a turbidimetric system for sandwich-type immunoassay using nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Y.H.; Lim, H.B., E-mail: plasma@dankook.ac.kr

    2015-07-09

    Graphical abstract: Laser-induced fluorescence reader with ratiometric correction for sandwich-type immunoassay using nanoparticles. - Highlights: • Laser-induced fluorescence system with ratiometric correction was developed. • The system reduced experimental error caused by particle loss and aggregation. • The detection limit of about 39 pg mL{sup −1} for salinomycin was obtained. • Calibration linearity and sensitivity were also significantly improved. • The system has the potential for bioanalysis using various nanoparticles. - Abstract: A unique laser-induced fluorescence (LIF) reader equipped with a turbidimetric system was developed for a sandwich-type immunoassay using nanoparticles. The system was specifically designed to reduce experimental error caused by particle loss, aggregation and sinking, and to improve analytical performance through ratiometric measurement of the fluorescence with respect to the turbidimetric absorbance. For application to determine the concentration of salinomycin, magnetic nanoparticles (MNPs) and FITC-doped silica nanoparticles (colored balls) immobilized with antibody were synthesized for magnetic extraction and for tagging as a fluorescence probe, respectively. The detection limit of about 39 pg mL{sup −1} was obtained, which was an improvement of about 2-fold compared to that obtained without employment of the turbidimetric system. Calibration linearity and sensitivity were also improved, with increase from 0.8601 to 0.9905 in the R{sup 2}-coefficient and by 1.92-fold for the curve slope, respectively. The developed LIF reader has the potential to be used for fluorescence measurements using various nanomaterials, such as quantum dots.

  14. Homogeneous plate based antibody internalization assay using pH sensor fluorescent dye.

    Science.gov (United States)

    Nath, Nidhi; Godat, Becky; Zimprich, Chad; Dwight, Stephen J; Corona, Cesear; McDougall, Mark; Urh, Marjeta

    2016-04-01

    Receptor-mediated antibody internalization is a key mechanism underlying several anti-cancer antibody therapeutics. Delivering highly toxic drugs to cancer cells, as in the case of antibody drug conjugates (ADCs), efficient removal of surface receptors from cancer cells and changing the pharmacokinetics profile of the antibody drugs are some of key ways that internalization impacts the therapeutic efficacy of the antibodies. Over the years, several techniques have been used to study antibody internalization including radiolabels, fluorescent microscopy, flow cytometry and cellular toxicity assays. While these methods allow analysis of internalization, they have limitations including a multistep process and limited throughput and are generally endpoint assays. Here, we present a new homogeneous method that enables time and concentration dependent measurements of antibody internalization. The method uses a new hydrophilic and bright pH sensor dye (pHAb dye), which is not fluorescent at neutral pH but becomes highly fluorescent at acidic pH. For receptor mediated antibody internalization studies, antibodies against receptors are conjugated with the pHAb dye and incubated with the cells expressing the receptors. Upon binding to the receptor, the dyes conjugated to the antibody are not fluorescent because of the neutral pH of the media, but upon internalization and trafficking into endosomal and lysosomal vesicles the pH drops and dyes become fluorescent. The enabling attributes of the pHAb dyes are the hydrophilic nature to minimize antibody aggregation and bright fluorescence at acidic pH which allows development of simple plate based assays using a fluorescent reader. Using two different therapeutic antibodies--Trastuzumab (anti-HER2) and Cetuximab (anti-EGFR)--we show labeling with pHAb dye using amine and thiol chemistries and impact of chemistry and dye to antibody ration on internalization. We finally present two new approaches using the pHAb dye, which will be

  15. Calibration of the linear response range of x-ray imaging plates and their reader based on image grayscale values

    Science.gov (United States)

    Ren, Kuan; Xu, Tao; Zheng, Jianhua; Dong, Jianjun; Wei, Minxi; Li, Chaoguang; Cao, Zhurong; Du, Huabing; Yan, Ji; Yang, Guohong; Yi, Rongqing; Zhang, Jiyan; Huang, Tianxuan; Liu, Shenye; Wang, Feng; Yang, Zhiwen; Li, Jin; Chen, Yaohua; Lan, Ke; Ren, Guoli; Liu, Jie; Ding, Yongkun; Jiang, Shaoen

    2017-08-01

    X-ray imaging plates are one of the most important X-ray imaging detectors and are widely used in inertial-confinement fusion experiments. However, their linear response range, which is the foundation of their quantitative data analysis, has not been sufficiently deeply investigated. In this work, we develop an X-ray fluorescer calibration system and carefully explore the linear response range of X-ray imaging plates. For the first time, nearly the entire grayscale range of the X-ray imaging plate linear response—7819-64 879 in the range of 0-65 535—has been observed. Further, we discuss the uncertainties involved in the calibration process. This work demonstrates the excellent linear response qualities of X-ray imaging plates and provides a significant foundation for expanding their quantitative applied range.

  16. Levels of Visual Stress in Proficient Readers: Effects of Spectral Filtering of Fluorescent Lighting on Reading Discomfort.

    Science.gov (United States)

    Loew, Stephen J; Rodríguez, Celestino; Marsh, Nigel V; Jones, Graham L; Núñez, Jose Carlos; Watson, Kenneth

    2015-08-10

    Visual stress (VS) affects reading in 5-12% of the general population and 31-36% of children with reading disorders. Symptoms include print distortions and visual discomfort when reading, and are exacerbated by fluorescent lighting. Prior research has indicated that VS can also affect proficient readers. We therefore examined levels of visual discomfort in a group of expert readers (n = 24) under both standard and spectrally-filtered fluorescent lighting. Participants rated their awareness of six symptoms of VS under each lighting condition. Under the standard condition, 4(16.7%) of the group recorded moderate to high levels of VS. Differences in symptom levels and reading speed between conditions were analysed using the Wilcoxon Signed Rank Test. Under the filter condition, the group reported less discomfort regarding all six symptoms of VS surveyed. The differences were significant with respect to three of the symptoms (p = .029 - p reading proficiency included significantly fewer self-corrections (p = .019) and total errors (p = .004). Here we present evidence that VS-type symptoms of reading discomfort are not confined to populations with reading difficulties and may also occur in proficient readers, and that simple adaptations to fluorescent lighting may alleviate such symptoms.

  17. Measuring intracellular calcium dynamics of HeLa cells exposed to nitric oxide by microplate fluorescence reader

    Science.gov (United States)

    Huang, Yimei; Chen, Jiangxu; Yang, Hongqin; Zheng, Liqin; Wang, Yuhua; Li, Hui; Xie, Shusen

    2012-12-01

    Nitric oxide (NO) has been reported to have the ability to promote or inhibit the proliferation and metastasis of cancer cells. It appears to have an effect on inducing calcium transient, which participates in essential cellular signaling in the physiological and pathological processes. Our work was intended to study the effects of exogenous NO on intracellular calcium dynamics of HeLa cells with Fluo-3, a calcium fluorescent indicator by microplate fluorescence reader. The results showed that after NO donor was injected into the wells, intracellular Ca2+ fluorescence intensity increased significantly compared with that of control group. Furthermore, the calcium transient activated by NO was mainly due to the calcium release from intracellular calcium stores. These would be helpful to further recognize the role of NO involved in cancer cell proliferation and metastasis.

  18. Dependence of fading patterns of photo-stimulated luminescence from imaging plates on radiation, energy, and image reader

    CERN Document Server

    Ohuchi, H

    2002-01-01

    We have been investigating the fading characteristics of imaging plates (IPs) as integral type detectors. The dependence on alpha, beta, and gamma ray radiation and their energies of the fading effect was measured using three types of IPs (BAS-UR, BAS-TR, and BAS-MS). The functions to correct the fading were determined by using the method reported in a previous paper. In all types of IPs, we confirmed that the fading effect is independent of the energy of the incident particles of beta and gamma rays and also independent of radiation except for the first component, which fades out in a very short time after irradiation with alpha rays. These results are very useful in the utilization of IPs as integral detectors in practical radiation fields. Empirically, the fading pattern is known to change when the IP is scanned by different types of image readers. The differences in the fading patterns obtained with two types of image readers, the BAS-1000 and the BAS-5000 (Fuji Film Co.), is discussed. Development of an ...

  19. Development of Plate Reader and On-Line Microfluidic Screening to Identify Ligands of the 5-Hydroxytryptamine Binding Protein in Venoms

    Directory of Open Access Journals (Sweden)

    Reka A. Otvos

    2015-06-01

    Full Text Available The 5-HT3 receptor is a ligand-gated ion channel, which is expressed in the nervous system. Its antagonists are used clinically for treatment of postoperative- and radiotherapy-induced emesis and irritable bowel syndrome. In order to better understand the structure and function of the 5-HT3 receptor, and to allow for compound screening at this receptor, recently a serotonin binding protein (5HTBP was engineered with the Acetylcholine Binding Protein as template. In this study, a fluorescence enhancement assay for 5HTBP ligands was developed in plate-reader format and subsequently used in an on-line microfluidic format. Both assay types were validated using an existing radioligand binding assay. The on-line microfluidic assay was coupled to HPLC via a post-column split which allowed parallel coupling to a mass spectrometer to collect MS data. This high-resolution screening (HRS system is well suitable for compound mixture analysis. As a proof of principle, the venoms of Dendroapsis polylepis, Pseudonaja affinis and Pseudonaja inframacula snakes were screened and the accurate masses of the found bioactives were established. To demonstrate the subsequent workflow towards structural identification of bioactive proteins and peptides, the partial amino acid sequence of one of the bioactives from the Pseudonaja affinis venom was determined using a bottom-up proteomics approach.

  20. A plate reader-based method for cell water permeability measurement

    DEFF Research Database (Denmark)

    Fenton, Robert A.; Moeller, H B; Nielsen, S

    2010-01-01

    Cell volume and water permeability measurements in cultured mammalian cells are typically conducted under a light microscope. Many of the employed approaches are time consuming and not applicable to a study of confluent epithelial cell monolayers. We present here an adaptation of a calcein-quenching-based......-mannitol concentrations. Similarly, according average cell volumes have been measured in suspension in a Coulter counter (particle-sizing device). Based on these measurements, we have derived an equation that facilitates the modeling of cell volume changes based on fluorescence intensity changes. We have utilized...

  1. Long-term optical stability of fluorescent solar concentrator plates

    NARCIS (Netherlands)

    Slooff, Lenneke H.; Bakker, Nicolaas J.; Sommeling, Paul M.; Büchtemann, Andreas; Wedel, Armin; Van Sark, Wilfried G J H M

    2014-01-01

    Fluorescent solar concentrators offer an alternative approach for low-cost photovoltaic energy conversion. For successful application, not only the power conversion efficiency and cost are important, but also lifetime or stability of the devices. As today's concentrator is made of polymer sheets

  2. Long-term optical stability of fluorescent solar concentrator plates

    NARCIS (Netherlands)

    Slooff, Lenneke H.; Bakker, Nicolaas J.; Sommeling, Paul M.; Büchtemann, Andreas; Wedel, Armin; Van Sark, Wilfried G J H M

    2014-01-01

    Fluorescent solar concentrators offer an alternative approach for low-cost photovoltaic energy conversion. For successful application, not only the power conversion efficiency and cost are important, but also lifetime or stability of the devices. As today's concentrator is made of polymer sheets con

  3. Electron energy dependence of characteristics of fluorescent plates for ultrahigh-voltage electron microscopes.

    Science.gov (United States)

    Nishi, R; Yoshida, K; Takaoka, A; Katsuta, T

    1996-03-01

    The characteristics of fluorescent plates for high energy electron beams (0.5-2.0 MeV) are examined. The thickness and the optical transparency of plates strongly affect the luminous broadening and intensity. The spatial luminous broadening in fluorescent plates is measured and is simply represented by the rise width of a knife edge image. When the thickness is much smaller than the range of incident electrons, the rise width is 1/4-1/5 of the thickness in the case of YAG single crystal plates that are transparent for light, while the rise width is nearly equal to the thickness for the packed P22 powder plates that are opaque for light. To suppress the luminous broadening under 50 microm, the thickness of YAG plates has to be thinner than 250 microm in the energy region around 2 MeV. Under the same condition of the rise width, the luminous intensity of YAG plates is twice as high as that of the P22 plates.

  4. Frequency domain fluorescence lifetime microwell-plate platform for respirometry measurements

    Science.gov (United States)

    Chatni, M. R.; Yale, G.; Van Ryckeghem, A.; Porterfield, D. M.

    2010-04-01

    Traditionally micro-well plate based platforms used in biology utilize fluorescence intensity based methods to measure processes of biological relevance. However, fluorescence intensity measurements suffer from calibration drift due to a variety of factors. Photobleaching and self-quenching of the fluorescent dyes cause the intensity signal to drop over the lifetime of sensor immobilized inside the well. Variation in turbidity of the sample during the course of the measurement affects the measured fluorescence intensity. In comparison, fluorescence lifetime measurements are not significantly affected by these factors because fluorescence lifetime is a physico-chemical property of the fluorescent dye. Reliable and inexpensive frequency domain fluorescence lifetime instrumentation platforms are possible because the greater tolerance for optical alignment, and because they can be performed using inexpensive light sources such as LEDs. In this paper we report the development of a frequency domain fluorescence lifetime well-plate platform utilizing an oxygen sensitive transition-metal ligand complex fluorophore with a lifetime in the microsecond range. The fluorescence lifetime dye is incorporated in a polymer matrix and immobilized on the base of micro-well of a 60 well micro-well plate. Respiration measurements are performed in both aqueous and non-aqueous environment. Respirometry measurements were recorded from single Daphnia magna egg in hard water. Daphnia is an aquatic organism, important in environmental toxicology as a standard bioassay and early warning indicator for water quality monitoring. Also respirometry measurements were recorded from Tribolium castaneum eggs, which are common pests in the processed flour industry. These eggs were subjected to mitochondrial electron transport chain inhibitor such as potassium cyanide (KCN) and its effects on egg respiration were measured in real-time.

  5. Fluorescence-PCR Assays and Isolation of Luminescent Bacterial Clones Using an Automated Plate Reader

    Science.gov (United States)

    Crowley, Thomas E.

    2011-01-01

    The genes responsible for luminescence in various species of the marine microorganism "Photobacterium", have been used for many years as a tool by researchers and instructors. In particular, the "lux" operon of "Photobacterium fischeri" has been used by many instructors to teach recombinant DNA techniques. Two methods using an automated plate…

  6. Fluorescence-PCR Assays and Isolation of Luminescent Bacterial Clones Using an Automated Plate Reader

    Science.gov (United States)

    Crowley, Thomas E.

    2011-01-01

    The genes responsible for luminescence in various species of the marine microorganism "Photobacterium", have been used for many years as a tool by researchers and instructors. In particular, the "lux" operon of "Photobacterium fischeri" has been used by many instructors to teach recombinant DNA techniques. Two methods using an automated plate…

  7. Dosimetry for ion-beam therapy using fluorescent nuclear track detectors and an automated reader

    CERN Document Server

    Greilich, Steffen; Klimpki, Grischa M; Kouwenberg, Jasper J M; Neuholz, Alexander; Pfeiler, Tina; Rahmanian, Shirin; Stadler, Alexander; Ulrich, Leonie

    2016-01-01

    For the assessment of effects of clinical ion-beams, dosimetry has to be complemented by information on particle-energy distribution or related quantities. Fluorescence nuclear track detectors made from C,Mg-doped alumina single crystals allow for the quantification of ion track density and energy loss on a single-track basis. In this study, their feasibility and accuracy to quantify fluence, linear-energy-transfer (LET) distributions, and eventually dose for a spread-out carbon ion Bragg peak was investigated. We found that while for the primary ions track densities agreed within a percent range with the reference data generated by Monte-Carlo radiation transport, the number of low-LET fragments in the beam was largely underestimated by approximately a factor three - the effect was most pronounced for protons where the measured fluence deviates at least an order of magnitude. Nevertheless, due to the dose major contribution of carbon ions, the determination of the individual detector sensitivity could be ide...

  8. High-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader.

    Science.gov (United States)

    Feng, Steve; Tseng, Derek; Di Carlo, Dino; Garner, Omai B; Ozcan, Aydogan

    2016-12-15

    Routine antimicrobial susceptibility testing (AST) can prevent deaths due to bacteria and reduce the spread of multi-drug-resistance, but cannot be regularly performed in resource-limited-settings due to technological challenges, high-costs, and lack of trained professionals. We demonstrate an automated and cost-effective cellphone-based 96-well microtiter-plate (MTP) reader, capable of performing AST without the need for trained diagnosticians. Our system includes a 3D-printed smartphone attachment that holds and illuminates the MTP using a light-emitting-diode array. An inexpensive optical fiber-array enables the capture of the transmitted light of each well through the smartphone camera. A custom-designed application sends the captured image to a server to automatically determine well-turbidity, with results returned to the smartphone in ~1 minute. We tested this mobile-reader using MTPs prepared with 17 antibiotics targeting Gram-negative bacteria on clinical isolates of Klebsiella pneumoniae, containing highly-resistant antimicrobial profiles. Using 78 patient isolate test-plates, we demonstrated that our mobile-reader meets the FDA-defined AST criteria, with a well-turbidity detection accuracy of 98.21%, minimum-inhibitory-concentration accuracy of 95.12%, and a drug-susceptibility interpretation accuracy of 99.23%, with no very major errors. This mobile-reader could eliminate the need for trained diagnosticians to perform AST, reduce the cost-barrier for routine testing, and assist in spatio-temporal tracking of bacterial resistance.

  9. High-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader

    Science.gov (United States)

    Feng, Steve; Tseng, Derek; di Carlo, Dino; Garner, Omai B.; Ozcan, Aydogan

    2016-12-01

    Routine antimicrobial susceptibility testing (AST) can prevent deaths due to bacteria and reduce the spread of multi-drug-resistance, but cannot be regularly performed in resource-limited-settings due to technological challenges, high-costs, and lack of trained professionals. We demonstrate an automated and cost-effective cellphone-based 96-well microtiter-plate (MTP) reader, capable of performing AST without the need for trained diagnosticians. Our system includes a 3D-printed smartphone attachment that holds and illuminates the MTP using a light-emitting-diode array. An inexpensive optical fiber-array enables the capture of the transmitted light of each well through the smartphone camera. A custom-designed application sends the captured image to a server to automatically determine well-turbidity, with results returned to the smartphone in ~1 minute. We tested this mobile-reader using MTPs prepared with 17 antibiotics targeting Gram-negative bacteria on clinical isolates of Klebsiella pneumoniae, containing highly-resistant antimicrobial profiles. Using 78 patient isolate test-plates, we demonstrated that our mobile-reader meets the FDA-defined AST criteria, with a well-turbidity detection accuracy of 98.21%, minimum-inhibitory-concentration accuracy of 95.12%, and a drug-susceptibility interpretation accuracy of 99.23%, with no very major errors. This mobile-reader could eliminate the need for trained diagnosticians to perform AST, reduce the cost-barrier for routine testing, and assist in spatio-temporal tracking of bacterial resistance.

  10. Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of Botulinum neurotoxin using high-affinity antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Warner, Marvin G.; Grate, Jay W.; Tyler, Abby J.; Ozanich, Richard M.; Miller, Keith D.; Lou, Jianlong; Marks, James D.; Bruckner-Lea, Cindy J.

    2009-09-01

    A fluorescence sandwich immunoassay using high affinity antibodies and quantum dot (QD) reporters has been developed for detection of botulinum toxin serotype A (BoNT/A). For the development of the assay, a nontoxic recombinant fragment of the holotoxin (BoNT/A-HC-fragment) has been used as a structurally valid simulant for the full toxin molecule. The antibodies used, AR4 and RAZ1, bind to nonoverlapping epitopes present on both the full toxin and on the recombinant fragment. In one format, the immunoassay is carried out in a 96-well plate with detection in a standard plate reader. Detection down to 31 pM of the BoNT/Hc-fragment was demonstrated with a total incubation time of 3 hours, using AR4 as the capture antibody and QD-coupled RAZ1 as the reporter. In a second format, the AR4 capture antibody was coupled to Sepharose beads, and the immunochemical reactions were carried out in microcentrifuge tubes with an incubation time of 1 hour. These beads were subsequently captured and concentrated in a rotating rod “renewable surface” flow cell as part of a sequential injection fluidic system. This flow cell was equipped with a fiber optic system for fluorescence measurements. In PBS buffer solution matrix, the BoNT/A-HC-fragment was detected to concentrations as low as 5 pM using the fluidic measurement approach.

  11. Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

    Directory of Open Access Journals (Sweden)

    Kensy Frank

    2009-06-01

    Full Text Available Abstract Background An advanced version of a recently reported high-throughput fermentation system with online measurement, called BioLector, and its validation is presented. The technology combines high-throughput screening and high-information content by applying online monitoring of scattered light and fluorescence intensities in continuously shaken microtiter plates. Various examples in calibration of the optical measurements, clone and media screening and promoter characterization are given. Results Bacterial and yeast biomass concentrations of up to 50 g/L cell dry weight could be linearly correlated to scattered light intensities. In media screening, the BioLector could clearly demonstrate its potential for detecting different biomass and product yields and deducing specific growth rates for quantitatively evaluating media and nutrients. Growth inhibition due to inappropriate buffer conditions could be detected by reduced growth rates and a temporary increase in NADH fluorescence. GFP served very well as reporter protein for investigating the promoter regulation under different carbon sources in yeast strains. A clone screening of 90 different GFP-expressing Hansenula polymorpha clones depicted the broad distribution of growth behavior and an even stronger distribution in GFP expression. The importance of mass transfer conditions could be demonstrated by varying filling volumes of an E. coli culture in 96 well MTP. The different filling volumes cause a deviation in the culture growth and acidification both monitored via scattered light intensities and the fluorescence of a pH indicator, respectively. Conclusion The BioLector technology is a very useful tool to perform quantitative microfermentations under engineered reaction conditions. With this technique, specific yields and rates can be directly deduced from online biomass and product concentrations, which is superior to existing technologies such as microplate readers or optode

  12. PLATE

    DEFF Research Database (Denmark)

    Kling, Joyce; Hjulmand, Lise-Lotte

    2008-01-01

    ’s level of English is sufficient for the increasing number of courses offered in English each semester. This paper addresses these concerns and describes a pilot project initiated in 2003 at CBS to gauge the overall English language proficiency of those teaching content courses in English. Through......Copenhagen Business School (CBS) finds itself needing to address the issue of English-medium instruction for its increasing number of foreign exchange and full degree students. With internationalisation as a main pillar of the institution’s agenda, there are concerns whether the teaching faculty...... the Project in Language Assessment for Teaching in English (PLATE) language professionals from CBS’s Language Center observe teachers and provide feedback using evaluation criteria from the Common European Framework for Reference (CEFR) supplemented by some additional criteria which take the LSP nature...

  13. PLATE

    DEFF Research Database (Denmark)

    Kling, Joyce; Hjulmand, Lise-Lotte

    2008-01-01

    Copenhagen Business School (CBS) finds itself needing to address the issue of English-medium instruction for its increasing number of foreign exchange and full degree students. With internationalisation as a main pillar of the institution’s agenda, there are concerns whether the teaching faculty......’s level of English is sufficient for the increasing number of courses offered in English each semester. This paper addresses these concerns and describes a pilot project initiated in 2003 at CBS to gauge the overall English language proficiency of those teaching content courses in English. Through...... the Project in Language Assessment for Teaching in English (PLATE) language professionals from CBS’s Language Center observe teachers and provide feedback using evaluation criteria from the Common European Framework for Reference (CEFR) supplemented by some additional criteria which take the LSP nature...

  14. A simple high-throughput method for determination of antiepileptic analogues of γ-aminobutyric acid in pharmaceutical dosage forms using microplate fluorescence reader.

    Science.gov (United States)

    Martinc, Boštjan; Vovk, Tomaž

    2013-01-01

    Pregabalin (PGB), gabapentin (GBP), and vigabatrin (VGB) are structural analogues of γ-aminobutyric acid used for the treatment of different forms of epilepsy. Their analytical determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been developed and used for their determination in bulk or pharmaceutical dosage forms. We aimed to develop a high- throughput method using a microplate reader with fluorescence detection and simple derivatization with fluorescamine. Obtained method involves derivatization step of only 5 min at room temperature and simultaneous measurements of 96 samples (λex 395, λem 476 nm) thus rendering excellent high-throughput analysis. The method was found to be linear with r²>0.998 across investigated analytical ranges of 0.75 to 30.0 µg/mL for PGB, 2.00 to 80.0 µg/mL for GBP, and 1.50 to 60.0 µg/mL for VGB. Intraday and interday precision values did not exceed 4.93%. The accuracy was ranging between 96.6 to 103.5%. The method was also found to be specific since used excipients did not interfere with the method. The robustness study showed that derivatization procedure is more robust than spectrofluorimetric conditions. The developed high-throughput method was successfully applied for determination of drug content and dissolution profiles in pharmaceutical dosage forms of studied antiepileptic drugs.

  15. COCHABAMBA READER.

    Science.gov (United States)

    LASTRA, YOLANDA

    INTENDED AS FOLLOWUP MATERIAL AFTER THE COMPLETION OF THE TWO-VOLUME SPOKEN COCHABAMBA COURSE, THIS READER CONSISTS OF A SINGLE LONG STORY, "JUANITO," WRITTEN BY OSCAR TERAN. IT HAS BEEN USED AS A RADIO SCRIPT FOR A SERIES OF BROADCASTS FROM A COCHABAMBA STATION WHICH SERVES THE SURROUNDING INDIGENOUS POPULATION. THE MATERIAL IS…

  16. Reader's Guide

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ Dear readers: This is the last issue for year 2002. Thanks to your consistent assistance and concerns, our magazine has accordingly made much an effort to objectively and genuinely introduce and reflect new achievements and climate by port sector across the country in terms of reform, construction, operation and development.

  17. Readers respond

    Directory of Open Access Journals (Sweden)

    Narendra N. Wig

    2005-11-01

    Full Text Available Please allow me to congratulate you on bringing out Mens Sana 8th and 9th Monographs on Medical Practice, Psychiatry and the Pharmaceutical Industry. You and Shakuntala have obviously put in an enormous effort to study dozens of documents, articles and books to bring out such a comprehensive review of this topic with focus on important areas for debate in India. You deserve congratulations of all the readers of these Monographs.

  18. Readers Respond

    Directory of Open Access Journals (Sweden)

    N.N. Wig

    2008-01-01

    Full Text Available This is in response to the MSM theme monograph Academia-Industry Symposium 2007: Medical Practice and the Pharmaceutical Industry: And Ever the Duo shall Meet.I recall that it was just five years ago when you first wrote to me, informing me about the start of Mens Sana Monographs . I was excited because it was something new, something different that was happening in the field of mental health and philosophy in India.You have come a long way since then. In fact, it is amazing how rapidly MSM has progressed, as testified to by the present theme monograph. Your readers and contributors are no longer confined to India; you are now international in reach and outlook. Please allow me to wholeheartedly congratulate you. It is a remarkable achievement by any standard.The present volume is excellent in its design and contents. The two editorials by Jerome Kassirer and Joel Lexchin are outstanding and deeply thought provoking. The article by Martin Van Der Weyden is equally rewarding and has very useful information. The main monograph has been superbly, very competently, and comprehensively written by both of you.In the series "What Medicine Means To Me," I especially liked the piece by Helen Herrman. Her message of public health psychiatry is particularly relevant for developing countries.The book review of David Healy's book by Leemon McHenry is very refreshing and challenging. I congratulate you on your decision to publish it when other journals were still hesitating.And lastly, the obituary reference to my dear friend Dr. Ravi Kapur by Ajit Bhide and you were so touching; I cried all over again.So keep up your good work, dear friends. May God bless you and may you both achieve many more successes in your careers.

  19. Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates

    Science.gov (United States)

    Maioli, Vincent; Chennell, George; Sparks, Hugh; Lana, Tobia; Kumar, Sunil; Carling, David; Sardini, Alessandro; Dunsby, Chris

    2016-11-01

    Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format.

  20. Reader Response in Perspective.

    Science.gov (United States)

    Sloan, Glenna

    2002-01-01

    Presents an overview of critical theory to show how the method of Reader Response evolved. Discusses theories of reading and the reader; variations within reader response; and implementing reader response in literacy programs. Includes a brief response by Robert E. Probst. (RS)

  1. Tuning a 96-Well Microtiter Plate Fluorescence-Based Assay to Identify AGE Inhibitors in Crude Plant Extracts

    Directory of Open Access Journals (Sweden)

    Luc Séro

    2013-11-01

    Full Text Available Advanced glycation end-products (AGEs are involved in the pathogenesis of numerous diseases. Among them, cellular accumulation of AGEs contributes to vascular complications in diabetes. Besides using drugs to lower blood sugar, a balanced diet and the intake of herbal products potentially limiting AGE formation could be considered beneficial for patients’ health. The current paper presents a simple and cheap high-throughput screening (HTS assay based on AGE fluorescence and suitable for plant extract screening. We have already implemented an HTS assay based on vesperlysines-like fluorescing AGEs quickly (24 h formed from BSA and ribose under physiological conditions. However, interference was noted when fluorescent compounds and/or complex mixtures were tested. To overcome these problems and apply this HTS assay to plant extracts, we developed a technique for systematic quantification of both vesperlysines (λexc 370 nm; λem 440 nm and pentosidine-like (λexc 335 nm; λem 385 nm AGEs. In a batch of medicinal and food plant extracts, hits were selected as soon as fluorescence decreased under a fixed threshold for at least one wavelength. Hits revealed during this study appeared to contain well-known and powerful anti-AGE substances, thus demonstrating the suitability of this assay for screening crude extracts (0.1 mg/mL. Finally, quercetin was found to be a more powerful reference compound than aminoguanidine in such assay.

  2. "Readers Forum" in Shanghai

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    On 23 April in Shanghai, a "Readers Forum" was held by Women of China to promote contact and exchangewith our readers and improve our work. It was a great honor that some of our foreign women readers, who areliving in Shanghai with their families on business at present, joined us, and nine of them made speeches at theforum. Moreover, two readers who couldn’t attend the forum in person, accepted a telephone interview. Shown hereare their kind comments and suggestions for our magazine.

  3. Teachers as Readers: Building Communities of Readers

    Science.gov (United States)

    Cremin, Teresa; Mottram, Marilyn; Collins, Fiona; Powell, Sacha; Safford, Kimberly

    2009-01-01

    Given the narrow scope of primary teachers' knowledge and use of children's literature identified in Phase I of "Teachers as Readers" (2006-2007), the core goal of the Phase II project was to improve teachers' knowledge and experience of such literature in order to help them increase children's motivation and enthusiasm for reading,…

  4. Our Readers and Friends

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    In Shanghai,a rapidly-developing modern metropolisthere are a lot of foreign women who have moved with theirfamilies to live and work there.During their leisure time theytake sightseeing trips and study Chinese culture,so theybecome readers and friends of our magazine.Recently,at ourReaders Forum in Shanghai,women from the UK,Belgium,the

  5. Romancing the YA Reader.

    Science.gov (United States)

    Charles, John; Mosley, Shelley; Bouricius, Ann

    1999-01-01

    Discusses the romance genre and provides guidelines for librarians with high school aged readers. Highlights include where to find information about the romance genre; the appeal of romance fiction; levels of sex and sensuality; reference tools; subgenres; and authors and titles that may be popular with young adult romance readers. (LRW)

  6. Dialect Readers Revisited.

    Science.gov (United States)

    Rickford, John R.; Rickford, Angela A.

    1995-01-01

    Presents the results of three experiments using dialect readers in African American Vernacular English (AAVE) to improve the reading comprehension of African American elementary and secondary school students. It is concluded that dialect readers represent a viable alternative for teaching AAVE speakers to read. (61 references) (MDM)

  7. Romancing the YA Reader.

    Science.gov (United States)

    Charles, John; Mosley, Shelley; Bouricius, Ann

    1999-01-01

    Discusses the romance genre and provides guidelines for librarians with high school aged readers. Highlights include where to find information about the romance genre; the appeal of romance fiction; levels of sex and sensuality; reference tools; subgenres; and authors and titles that may be popular with young adult romance readers. (LRW)

  8. To Our Readers

    Institute of Scientific and Technical Information of China (English)

    Editor in Ch

    2006-01-01

    @@ Contemporary International Relations, the English journal published by China Institutes of Contemporary International Relations, is now 15 years old. Since its conception as the first Chinese journal in English covering the field of international relations, it has presented to our readers numerous articles written by the research fellows of our institutes on the subjects of international affairs, international relations, and international relations theories. We have received numerous letters of encouragement and positive comments. We have also gotten letters in which our readers have given their opinions on what they would like to see in this journal. Some readers pointed out to us the mistakes in the translation. Other readers noted the occasional delay in delivery or even missing issues. We are grateful to those who have always supported us. We also feel the need to improve for those who have not been totally satisfied with our journal.

  9. To our Readers

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Contemporary International Relations, the English journal published by China Institutes of Contemporary International Relations, is now 15 years old. Since its conception as the first Chinese journal in English covering the field of international relations, it has presented to our readers numerous articles written by the research fellows of our institutes on the subjects of international affairs, international relations, and international relations theories. We have received numerous letters of encouragement and positive comments. We have also gotten letters in which our readers have given their opinions on what they would like to see in this journal. Some readers pointed out to us the mistakes in the translation. Other readers noted the occasional delay in delivery or even missing issues. We are grateful to those who have always supported us. We also feel the need to improve for those who have not been totally satisfied with our journal.

  10. To Our Readers

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Contemporary International Relations, the English journal published by China Institutes of Contemporary International Relations, is now 15 years old. Since its conception as the first Chinese journal in English covering the field of international relations, it has presented to our readers numerous articles written by the research fellows of our institutes on the subjects of international affairs, international relations, and international relations theories. We have received numerous letters of encouragement and positive comments. We have also gotten letters in which our readers have given their opinions on what they would like to see in this journal. Some readers pointed out to us the mistakes in the translation. Other readers noted the occasional delay in delivery or even missing issues. We are grateful to those who have alwayssupported us. We also feel the need to improve for those who have not been totally satisfied with our journal.

  11. Letters from Our Readers

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    Since the first publication of Cultural Exchang-China& World in October 1992,we have received a unmber of verykind letters from Chinese and foreign readers giving support andencouagement to this magazine.We would like to take thisopportunity to thank them all and to invite others to write in as well,especially if they have suggestions or criticisms to make.Thefollowing are letters from two of our overseas readers.

  12. PANJABI READER, LEVEL 2.

    Science.gov (United States)

    VATUK, VED P.

    THIS SECOND-LEVEL READER IN THE PANJABI LANGUAGE CAN BE USED IN A SECOND- OR THIRD-YEAR COURSE AS A SUPPLEMENT TO CONVERSATIONAL MATERIALS, OR BY ITSELF IN A COURSE ON THE WRITTEN LANGUAGE. THE GRAMMAR APPENDIX INCLUDED IS IDENTICAL TO THAT FOUND IN THE FIRST-LEVEL PANJABI READER (ED 010 485). THE MAIN PORTION OF THE BOOK CONSISTS OF 23 UNITS,…

  13. Reader Response Approaches and the Growth of Readers.

    Science.gov (United States)

    Spiegel, Dixie Lee

    1998-01-01

    Outlines what reader response might look like in the classroom. Presents four basic assumptions of reader response theory. Discusses ways reader response approaches to literature help students grow both in the depth and breadth of their responses to literature, and as strategic readers. (SR)

  14. An easy ratiometric compensation for the extracellular Ca2+ indicator-caused fluorescence artifact.

    Science.gov (United States)

    Kukkonen, Jyrki P

    2009-07-15

    Measurement of intracellular Ca(2+) dynamics is one of the most central real-time assays for cellular signaling. Ratiometric methods reduce the need for internal calibration and also effectively compensate for most artifacts when used in imaging. However, ratiometric calculation cannot compensate for extracellularly leaked (and fluorescent) Ca(2+) indicator and will instead indicate erroneous Ca(2+) concentration. This frequently occurs in systems where extracellular indicator is accumulated such as fluorescence spectrophotometers and plate readers. Here I present a method that, for the first time, fully compensates for this phenomenon. The method uses a single-step internal calibration together with a predefined ratiometric calibration protocol.

  15. The Bakhtin Reader The Bakhtin Reader

    Directory of Open Access Journals (Sweden)

    Helen Reid Thomas

    2008-04-01

    Full Text Available The publication of Caryl Emerson’s book The First Hundred Years of Mikhail Bakhtin in December 1997 is an indication that interest in Bakhtin continues to flourish, among both Russian and Western scholars. Emerson’s book traces the interesting differences of attitude and emphasis between these two groups of critics and provides a broad-ranging and scholarly history of Bakhtin studies. The book I am concerned with here, The Bakhtin Reader, though of a different nature and designed for a different audience, is perhaps an even more significant testament to Bakhtin’s extraordinary and continuing influence. The publication of Caryl Emerson’s book The First Hundred Years of Mikhail Bakhtin in December 1997 is an indication that interest in Bakhtin continues to flourish, among both Russian and Western scholars. Emerson’s book traces the interesting differences of attitude and emphasis between these two groups of critics and provides a broad-ranging and scholarly history of Bakhtin studies. The book I am concerned with here, The Bakhtin Reader, though of a different nature and designed for a different audience, is perhaps an even more significant testament to Bakhtin’s extraordinary and continuing influence.

  16. Capacitive label reader

    Science.gov (United States)

    Arlowe, H. Duane

    1985-01-01

    A capacitive label reader includes an outer ring transmitting portion, an inner ring transmitting portion, and a plurality of insulated receiving portions. A label is the mirror-image of the reader except that identifying portions corresponding to the receiving portions are insulated from only one of two coupling elements. Positive and negative pulses applied, respectively, to the two transmitting rings biased a CMOS shift register positively to either a 1 or 0 condition. The output of the CMOS may be read as an indication of the label.

  17. Mid-frequency readers

    OpenAIRE

    Nation, Ian Stephen Paul; Anthony, Laurence

    2013-01-01

    This article describes a new free extensive reading resource for learning the mid-frequency words of English and for reading well known texts with minor vocabulary adaptation. A gap exists between the end of graded readers at around 3,000 word families and the vocabulary size needed to read unsimplified texts at around 8,000 word families. Mid-frequency readers are designed to fill this gap. They consist of texts from Project Gutenberg adapted for learners with a vocabulary size of 4,000 word...

  18. PANJABI READER. LEVEL 1.

    Science.gov (United States)

    VATUK, VED P.

    A FIRST-LEVEL READER IS PRESENTED, PRIMARILY FOR THOSE STUDENTS WHO HAVE A SPEAKING KNOWLEDGE OF PANJABI AND SOME KNOWLEDGE OF PANJABI GRAMMAR. THIS VOLUME CAN BE USED IN A GENERAL PANJABI LANGUAGE COURSE AS A SUPPLEMENT TO CONVERSATIONAL MATERIALS, OR BY ITSELF IN A COURSE ON THE WRITTEN LANGUAGE. A GLOSSARY AND A BRIEF GRAMMATICAL APPENDIX HAVE…

  19. Understanding Readers' Differing Understandings

    Science.gov (United States)

    Kucer, Stephen B.

    2015-01-01

    This research examines the characteristics of reader understandings that vary from those stated in the text. Eighty-seven fourth graders orally read complex academic literary and scientific texts, followed by probed retellings. Retold ideas not directly supported by, or reflective of, the texts were identified. These differing understandings…

  20. Rockin' Readers Handbook.

    Science.gov (United States)

    Alachua County Schools, Gainesville, FL.

    Designed for use by volunteers in the program, this handbook describes the "Rockin' Readers" program, in which senior-citizen volunteers are matched with specifically targeted at-risk children in Alachua County, Florida, who tested below their peer group in language development and reading readiness skills. The handbook notes that each…

  1. Rockin' Readers Coordinator Handbook.

    Science.gov (United States)

    Alachua County Schools, Gainesville, FL.

    This coordinator's handbook describes the "Rockin' Readers" program, in which senior-citizen volunteers are matched with specifically targeted at-risk children (usually of kindergarten age or slightly older) in Alachua County, Florida, who tested below their peer group in language development and reading readiness skills. The handbook…

  2. Readers of histone modifications

    Institute of Scientific and Technical Information of China (English)

    Miyong Yun; Jun Wu; Jerry L Workman; Bing Li

    2011-01-01

    Histone modifications not only play important roles in regulating chromatin structure and nuclear processes but also can be passed to daughter cells as epigenetic marks.Accumulating evidence suggests that the key function of histone modifications is to signal for recruitment or activity of downstream effectors. Here, we discuss the latest discovery of histone-modification readers and how the modification language is interpreted.

  3. The assessment of writing ability: Expert readers versus lay readers

    NARCIS (Netherlands)

    Schoonen, R.; Vergeer, M.; Eiting, M.

    1997-01-01

    This article reports on three studies about the reading reliability of lay and expert readers in rating three kinds of writing assignments. Readers had to rate the Con tent and Language Usage of students' writing performances. The studies show that expert readers are more reliable in rating Usage, w

  4. Readers' Readings: Applications of Reader-Response Theory.

    Science.gov (United States)

    Steiner, Linda

    In the interest of applying reader response theory to journalism this paper posits that readers of newspapers, like readers of literature, take an active role in making meaning from the articles they read, rather than passively accepting news as a finished, static product. Additionally, it proposes that journalism textbooks pay little attention to…

  5. Crash course in readers' advisory

    CERN Document Server

    Orr, Cynthia

    2014-01-01

    One of the key services librarians provide is helping readers find books they'll enjoy. This ""crash course"" will furnish you with the basic, practical information you need to excel at readers' advisory (RA) for adults and teens.

  6. To Our Readers

    Institute of Scientific and Technical Information of China (English)

    Editor in Chief

    2006-01-01

    @@ Contemporary International Relations, the English journal published by China Institutes of Contemporary International Relations, is now 15 years old. Since its conception as the first Chinese journal in English covering the field of international relations, it has presented to our readers numerous articles written by the research fellows of our institutes on the subjects of international affairs, international relations, and international relations theories. We have received numerous letters of encouragement and positive comments.

  7. Bytes, Books and Readers

    DEFF Research Database (Denmark)

    Rasmussen, Krista Stinne Greve

    2016-01-01

    Geschichte von Editionen und der Entwicklung der Digital Humanities besonderes Augenmerk geschenkt, um zu demonstrieren, wie bedeutsam die Analyse der Überlieferung und der Textbegriff für die Geschichte von Editionen sind. Beispiele aus Alastair McKinnons erster digitaler Edition von Soren Kierkegaards......In diesem Beitrag werden Ergebnisse aus der Dissertation der Autorin Bytes, Books, and Readers. A Historical Analysis of the Transition from Printed to Digital Scholarly Editions Focusing on ‘The Writings of Søren Kierkegaard’ (2015) vorgestellt. Dabei wird dem Wechselverhältnis zwischen der...

  8. Chipless RFID reader architecture

    CERN Document Server

    Karmakar, Nemai Chandra; Kalansuriya, Prasanna

    2013-01-01

    In the era of information communication technology (ICT), radio frequency identification (RFID) has been going through tremendous development. RFID technology has the potential of replacing barcodes due to its large information carrying capacity, flexibility in operations, and applications. The deployment of RFID has been hindered by its cost. However, with the advent of low powered ICs, energy scavenging techniques, and low-cost chipless tags, RFID technology has achieved significant development. This book addresses the new reader architecture, presents fundamentals of chipless RFID systems,

  9. Narrative tension analysis: reader`s and text aspects

    Directory of Open Access Journals (Sweden)

    Анна Вениаминовна Лещенко

    2016-12-01

    Full Text Available In this article narrative tension is conceptualized as a complex cognitive and affective phenomenon being analysed in two dimensions: psychological and textological. In the receptive and psychological framework narrative tension is considered as reader`s psychological response to the fictional events of the storyworld. In modern text theory narrative tension is thought as a specific narrative strategy represented by definite narrative structures inducing the particular emotional state of the reader

  10. Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection

    Directory of Open Access Journals (Sweden)

    Axel eDurand

    2013-01-01

    Full Text Available We have developed a method for the determination of copper in natural waters at nanomolar levels. The use of a microplate-reader minimises sample processing time (~ 25 sec per sample, reagent consumption (~ 120 μL per sample and sample volume (~ 700 μL. Copper is detected by chemiluminescence. This technique is based on the formation of a complex between copper and 1,10-phenanthroline and the subsequent emission of light during the oxidation of the complex by hydrogen peroxide. Samples are acidified to pH 1.7 and then introduced directly into a 24-well plate. Reagents are added during data acquisition via two reagent injectors. When trace metal clean protocols are employed, the reproducibility is generally less then 7% on blanks and the detection limit is 0.7 nM for seawater and 0.4 nM for freshwater. More than 100 samples per hour can be analyzed with this technique, which is simple, robust, and amenable to at-sea analysis. Seawater samples from Storm Bay in Tasmania illustrate the utility of the method for environmental science. Indeed other trace metals for which optical detection methods exist (e.g. chemiluminescence, fluorescence and absorbance could be adapted to the microplate-reader.

  11. Analysis of the contribution of sedimentation to bacterial mass transport in a parallel plate flow chamber Part II : Use of fluorescence imaging

    NARCIS (Netherlands)

    Li, Jiuyi; Busscher, Henk J.; van der Mei, Henny C.; Norde, Willem; Krom, Bastiaan P.; Sjollema, Jelmer

    2011-01-01

    Using a new phase-contrast microscopy-based method of analysis, sedimentation has recently been demonstrated to be the major mass transport mechanism of bacteria towards substratum surfaces in a parallel plate flow chamber (J. Li, H.J. Busscher, W. Norde, J. Sjollema, Colloid Surf. B. 84 (2011)76).

  12. Analysis of the contribution of sedimentation to bacterial mass transport in a parallel plate flow chamber Part II: Use of fluorescence imaging

    NARCIS (Netherlands)

    Li, J.Y.; Busscher, H.J.; Mei, van der H.C.; Norde, W.; Krom, B.P.; Sjollema, J.

    2011-01-01

    Using a new phase-contrast microscopy-based method of analysis, sedimentation has recently been demonstrated to be the major mass transport mechanism of bacteria towards substratum surfaces in a parallel plate flow chamber (J. Li, H.J. Busscher, W. Norde, J. Sjollema, Colloid Surf. B. 84 (2011)76).

  13. To Our Readers

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    Golden October is a harvest month in China,bringing hope andjoy to people,so it is an appropriate time for us to dedicate,withgood wishes and deep friendship,Cultural Exchange—China World to both Chinese and foreign readers.Today the rising tide of reform and opening to the outside world inChina impels Chinese to pursue novelty and development.Looking tothe future,the Chinese are creating a beautiful life for themselves withunprecedented enthusiasm and ambition.This magazine is born at a timewhen exchanges in various fields between the Chinese people and thepeoples of the world are increasing and relationships are becoming closerand closer.The magazine will work to promote Chinese and foreigncultural exchanges.

  14. Magnetic Symbology Reader

    Science.gov (United States)

    Schramm, Harry F. (Inventor); Shih, William C. L. (Inventor); Fitzpatrick, Gerald L. (Inventor); Knisely, Craig (Inventor)

    2004-01-01

    A magnetic symbology reader has a housing containing a polarized light source which directs light though a magneto-optic sensor onto a reflector which reflects light least one analyzer and into at least one camera. A view finder allows the user to monitor the image on the sensor as seen by a viewfinder camera while a processor is coupled to possibly a second camera so that when an image is detected, the image from the camera may be processed by the processor to output information associated with the symbol to an external source. The analyzer and polarized light source provide contrast in the images detected by the sensor. A bias/erase coil located about the magneto-optic sensor can enhance or erase images on the sensor.

  15. Putting the pieces together: contribution of fluorescence polarization assays to small-molecule lead optimization

    Science.gov (United States)

    Keating, Susan M.; Marsters, Jim; Beresini, Maureen; Ladner, Carmen; Zioncheck, Kim; Clark, Kevin; Arellano, Fred; Bodary, Sarah

    2000-04-01

    Fluorescence polarization assays with both purified receptor and intact cells have been developed to assess potency and selectivity of antagonists of the interaction of the lymphocyte receptor, LFA-1, and its endothelial ligand, ICAM-1. Fluorescein isothiocyanate conjugated small molecule probes were optimized for use in binding assay with LFA-1 and a closely related receptor, MAC-1. In the assays, the antagonists compete with the fluorescent probe for binding to the receptor. This enables the determination of IC50 and consequently Ki values of the antagonists for each of the receptors. Routine use of polarization assay with tranfected cells, in addition to purified receptors, has become feasible with the availability of sensitive plate readers that are able to detect 1 nM fluorescent probe in 15 (mu) l sample volumes with good signal to noise. These measurements aid in the iterative synthesis of more potent and selective compounds.

  16. Readers Responding--And Then?

    Science.gov (United States)

    Hansson, Gunnar

    1992-01-01

    Argues that recognition of the reader's role in the production of meanings in literary works has major consequences for the theory, research, and teaching of literature. Discusses the need to include the reader's role when writing the history of literature; develop new language for the description of the meanings of works; and develop new models…

  17. THE E-READER BOOM

    Institute of Scientific and Technical Information of China (English)

    YU SHUJUN

    2010-01-01

    @@ The fire Amazon.com Inc.'s Kindle has kindled in the United States has spread to China. But it's not a buying spree of Kindle products, which aren't being sold here, but a manufacturing frenzy of Kindle-like products——electronicbook readers (e-readers).

  18. Shifting Perspectives on Struggling Readers.

    Science.gov (United States)

    Primeaux, Joan

    2000-01-01

    Draws on social constructivist theory to present principles of reading instruction for struggling readers. Focuses on three social constructivist-based components that have been used successfully with struggling readers to enhance their reading instruction: a responsive literacy environment, explicit comprehension-strategy instruction, and time…

  19. The Routledge Applied Linguistics Reader

    Science.gov (United States)

    Wei, Li, Ed.

    2011-01-01

    "The Routledge Applied Linguistics Reader" is an essential collection of readings for students of Applied Linguistics. Divided into five sections: Language Teaching and Learning, Second Language Acquisition, Applied Linguistics, Identity and Power and Language Use in Professional Contexts, the "Reader" takes a broad interpretation of the subject…

  20. The Routledge Applied Linguistics Reader

    Science.gov (United States)

    Wei, Li, Ed.

    2011-01-01

    "The Routledge Applied Linguistics Reader" is an essential collection of readings for students of Applied Linguistics. Divided into five sections: Language Teaching and Learning, Second Language Acquisition, Applied Linguistics, Identity and Power and Language Use in Professional Contexts, the "Reader" takes a broad…

  1. Image based quantitative reader for Lateral flow immunofluorescence assay.

    Science.gov (United States)

    Chowdhury, Kaushik Basak; Joseph, Jayaraj; Sivaprakasam, Mohanasankar

    2015-08-01

    Fluorescence Lateral flow immunoassays (LFIA) have wide range of applications in point-of-care testing (POCT). An integrated, motion-free, accurate, reliable reader that performs automated quantitative analysis of LFIA is essential for POCT diagnosis. We demonstrate an image based quantitative method to read the lateral flow immunofluorescence test strips. The developed reader uses line laser diode module to illuminate the LFIA test strip having fluorescent dye. Fluorescence light coming from the region of interest (ROI) of the LFIA test strip was filtered using an emission filter and imaged using a camera following which images were processed in computer. A dedicated control program was developed that automated the entire process including illumination of the test strip using laser diode, capturing the ROI of the test strip, processing and analyzing the images and displaying of results. Reproducibility of the reader has been evaluated using few reference cartridges and HbA1c (Glycated haemoglobin) test cartridges. The proposed system can be upgraded to a compact reader for widespread testing of LFIA test strips.

  2. Characterization of the commercially-available fluorescent chloroquine-BODIPY conjugate, LynxTag-CQGREEN, as a marker for chloroquine resistance and uptake in a 96-well plate assay.

    Directory of Open Access Journals (Sweden)

    Cheryl C Y Loh

    Full Text Available Chloroquine was a cheap, extremely effective drug against Plasmodium falciparum until resistance arose. One approach to reversing resistance is the inhibition of chloroquine efflux from its site of action, the parasite digestive vacuole. Chloroquine accumulation studies have traditionally relied on radiolabelled chloroquine, which poses several challenges. There is a need for development of a safe and biologically relevant substitute. We report here a commercially-available green fluorescent chloroquine-BODIPY conjugate, LynxTag-CQGREEN, as a proxy for chloroquine accumulation. This compound localized to the digestive vacuole of the parasite as observed under confocal microscopy, and inhibited growth of chloroquine-sensitive strain 3D7 more extensively than in the resistant strains 7G8 and K1. Microplate reader measurements indicated suppression of LynxTag-CQGREEN efflux after pretreatment of parasites with known reversal agents. Microsomes carrying either sensitive- or resistant-type PfCRT were assayed for uptake; resistant-type PfCRT exhibited increased accumulation of LynxTag-CQGREEN, which was suppressed by pretreatment with known chemosensitizers. Eight laboratory strains and twelve clinical isolates were sequenced for PfCRT and Pgh1 haplotypes previously reported to contribute to drug resistance, and pfmdr1 copy number and chloroquine IC50s were determined. These data were compared with LynxTag-CQGREEN uptake/fluorescence by multiple linear regression to identify genetic correlates of uptake. Uptake of the compound correlated with the logIC50 of chloroquine and, more weakly, a mutation in Pgh1, F1226Y.

  3. Narrative tension analysis: reader`s and text aspects

    OpenAIRE

    Анна Вениаминовна Лещенко

    2016-01-01

    In this article narrative tension is conceptualized as a complex cognitive and affective phenomenon being analysed in two dimensions: psychological and textological. In the receptive and psychological framework narrative tension is considered as reader`s psychological response to the fictional events of the storyworld. In modern text theory narrative tension is thought as a specific narrative strategy represented by definite narrative structures inducing the particular emotional state of the ...

  4. Choosing the Right Basal Reader.

    Science.gov (United States)

    Robbins, Ruth H.

    1980-01-01

    Looks at factors in the textbook publishing industry, especially costs and censorship, which affect the quality of available reading series. Notes the problem of readability and content bias. Finally, presents a checklist of basal reader evaluation criteria. (SJL)

  5. Measuring with the spiral reader

    CERN Multimedia

    1974-01-01

    The spiral reader shown here was at the time, together with the Shivamatic scanning system, the basic equipment used for measuring bubble chamber pictures. Anne Anton sits at the table. (See Photo Archive 7408343.)

  6. Writing for the Invisible Reader.

    Science.gov (United States)

    Garden, Nancy

    2000-01-01

    Discusses the role of the lesbian writer in addressing the invisibility of teenage readers. Argues that writing about homosexuality is a matter of presenting it rather than promoting it. Includes a review of "The Year they Burned the Books." (NH)

  7. Readers Theatre: A different approach to English for struggling readers

    Directory of Open Access Journals (Sweden)

    Ion Drew

    2010-09-01

    Full Text Available This article presents a study of the use of Readers Theatre in English lessons with groups of academically-challenged pupils in a Norwegian lower secondary school. The study is based on the teacher’s logs, interviews with the teacher, a questionnaire answered by the pupils, and lesson observations. Readers Theatre, a group reading activity that can be used with a wide range of texts, was successfully incorporated into the curriculum with relatively small ‘fordypning’ (specialisation groups in English. These pupils had opted for more English lessons instead of learning a second foreign language. Most of them struggled with English as their first foreign language and were struggling readers. However, the majority of the pupils experienced Readers Theatre as both enjoyable and educational. The experience had a positive effect on their confidence and motivation to read. It also helped to improve their reading fluency and accuracy, for example pronunciation, and facilitated growth in vocabulary. The experience of practising and performing as a group was especially satisfying and motivating for the pupils involved. The majority were keen to participate in other Readers Theatre projects.

  8. Introduction to fluorescence

    CERN Document Server

    Jameson, David M

    2014-01-01

    "An essential contribution to educating scientists in the principles of fluorescence. It will also be an important addition to the libraries of practitioners applying the principles of molecular fluorescence."-Ken Jacobson, Kenan Distinguished Professor of Cell Biology and Physiology, University of North Carolina at Chapel Hill"An exquisite compendium of fluorescence and its applications in biochemistry enriched by a very exciting historical perspective. This book will become a standard text for graduate students and other scientists."-Drs. Zygmunt (Karol) Gryczynski and Ignacy Gryczynski, University of North Texas Health Science Center"… truly a masterwork, combining clarity, precision, and good humor. The reader, novice or expert, will be pleased with the text and will not stop reading. It is a formidable account of the fluorescence field, which has impacted the life sciences so considerably in the last 60 years."-Jerson L. Silva, M.D., Ph.D., Professor and Director, National Institute of Science and Tech...

  9. Readers' Theatre as a History Teaching Tool.

    Science.gov (United States)

    Harmon, Sandra D.; Riney-Kehrberg, Pamela; Westbury, Susan

    1999-01-01

    Describes the production of a readers' theater version of the first women's rights convention held at Seneca Falls, New York (includes script). Discusses the benefits of readers' theater for studying women's history and encouraging female student class participation; strategies for using readers' theater; and students creating their own readers'…

  10. Three Properties of the Ideal Reader.

    Science.gov (United States)

    Kay, Paul

    The main experience of an ideal reader while reading a text is an "envisionment" of that text, a representation in the reader's mind of the content of the text. According to this view the envisionment grows and sometimes changes as the reader progresses through the text, and the ideal reader not only updates and supplements the…

  11. Medically Relevant Assays with a Simple Smartphone and Tablet Based Fluorescence Detection System

    Directory of Open Access Journals (Sweden)

    Piotr Wargocki

    2015-05-01

    Full Text Available Cell phones and smart phones can be reconfigured as biomedical sensor devices but this requires specialized add-ons. In this paper we present a simple cell phone-based portable bioassay platform, which can be used with fluorescent assays in solution. The system consists of a tablet, a polarizer, a smart phone (camera and a box that provides dark readout conditions. The assay in a well plate is placed on the tablet screen acting as an excitation source. A polarizer on top of the well plate separates excitation light from assay fluorescence emission enabling assay readout with a smartphone camera. The assay result is obtained by analysing the intensity of image pixels in an appropriate colour channel. With this device we carried out two assays, for collagenase and trypsin using fluorescein as the detected fluorophore. The results of collagenase assay with the lowest measured concentration of 3.75 µg/mL and 0.938 µg in total in the sample were comparable to those obtained by a microplate reader. The lowest measured amount of trypsin was 930 pg, which is comparable to the low detection limit of 400 pg for this assay obtained in a microplate reader. The device is sensitive enough to be used in point-of-care medical diagnostics of clinically relevant conditions, including arthritis, cystic fibrosis and acute pancreatitis.

  12. Genipin-induced changes in collagen gels: correlation of mechanical properties to fluorescence.

    Science.gov (United States)

    Sundararaghavan, Harini G; Monteiro, Gary A; Lapin, Norman A; Chabal, Yves J; Miksan, Jennifer R; Shreiber, David I

    2008-11-01

    Controlled crosslinking of collagen gels has important applications in cell and tissue mechanics as well as tissue engineering. Genipin is a natural plant extract that has been shown to crosslink biological tissues and to produce color and fluorescence changes upon crosslinking. We have characterized the effects of genipin concentration and incubation duration on the mechanical and fluorigenic properties of type I collagen gels. Gels were exposed to genipin (0, 1, 5, or 10 mM) for a defined duration (2, 4, 6, or 12 h). Mechanical properties were characterized using parallel plate rheometry, while fluorigenic properties were examined with a spectrofluorimetric plate reader and with a standard, inverted epifluorescent microscope. Additionally, Fourier transform infrared spectroscopy was used to characterize and track the crosslinking reaction in real-time. Genipin produced significant concentration- and incubation-dependent increases in the storage modulus, loss modulus, and fluorescence intensity. Storage modulus was strongly correlated to fluorescence exponentially. Minimal cytotoxicity was observed for exposure of L929 fibroblasts cultured within collagen gels to 1 mM genipin for 24 h, but significant cell death occurred for 5 and 10 mM genipin. We conclude that genipin can be used to stiffen collagen gels in a relatively short time frame, that low concentrations of genipin can be used to crosslink cell-populated collagen gels to affect cell behavior that is influenced by the mechanical properties of the tissue scaffold, and that the degree of crosslinking can be reliably assayed optically via simple fluorescence measurements.

  13. A rapid and sensitive method to detect mycobacterium tuberculosis DNA by fluorescence polarization

    Institute of Scientific and Technical Information of China (English)

    白玉杰; 赵锦荣; 薛丽; 刘爱翔; 张文红; 郭晏海; 闫小君

    2004-01-01

    Objective: To develop a new high sensitivity, rapid and simple mycobacterium tuberculosis DNA detection method using fluorescence polarization technology. Methods: In our asymmetric PCR protocol, 100 times mole of TB-R primer was added than in the usual symmetric PCR to get enough single strands PCR product. The probe TB-5'-TAMRA and PCR products were mixed in a tube and incubate for 5 - 15 min at 46 C. The polarization (mp) was measured using victor2Multilabel Plate Reader. Results: Asymmetric and symmetric PCR products were analyzed by the FP method. Asymmetric PCR products are detected more sensitively than symmetric ones. The polarization values of probe associated with asymmetric products were much higher than with symmetric products. Conclusion: This fluorescence polarization assay in conjunction with asymmetric PCR is a powerful and widely applicable method for the rapid and sensitive detection of micro-organisms in clinical laboratories.

  14. Readers' Knowledge of Popular Genre

    Science.gov (United States)

    Dixon, Peter; Bortolussi, Marisa

    2009-01-01

    This research examined readers' knowledge of popular genres. Participants wrote short essays on fantasy, science fiction, or romance. The similarities among the essays were measured using latent semantic analysis (LSA) and were then analyzed using multidimensional scaling and cluster analysis. The clusters and scales were interpreted by searching…

  15. Enduring and Diagnosing Reader's Block.

    Science.gov (United States)

    Melanson, Lisa Stapleton

    1990-01-01

    Describes a condition called "reader's block" whereby the mind fails to comprehend the meaning of the text because of digressing thoughts. Suggests that "freereading," like freewriting, can help to clarify thoughts. Argues that it is not necessary to read things correctly the first time through. (PRA)

  16. Writing Reviews for Readers' Advisory

    Science.gov (United States)

    Hooper, Brad

    2010-01-01

    Reviews are an important resource for readers' advisory and collection development. They are also a helpful promotional tool, introducing patrons to what is new on the shelf. This resource includes: (1) Tips for writing strong, relevant reviews; (2) Different ways reviews can be used to promote your library; and (3) A chapter by Joyce Saricks…

  17. Postmodern theories about readers in electronic environment

    OpenAIRE

    Ivanka Kuić

    2015-01-01

    Introductory part of the paper discusses theories about readers in the last decades of the 20th century. In particular, two big theoretical schools are discussed: aesthetic reception theory and reader-response-criticism movement. Readers are a subject of very different scientific disciplines: literature theory, sociology, anthropology, book history and library science. The idea that a reader is an essential subject for future life of a literary work is common to all theorists. By constructing...

  18. 38 CFR 21.150 - Reader service.

    Science.gov (United States)

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Reader service. 21.150... Special Rehabilitation Services § 21.150 Reader service. (a) Limitations on vision. A veteran considered to have a visual impairment necessitating reader service includes a veteran: (1) Whose best corrected...

  19. Moving Readers from Struggling to Proficient

    Science.gov (United States)

    Wolter, Deborah

    2017-01-01

    If we want to move children from struggling to read to being proficient readers, we must address the disparate ways that teachers respond to readers with varying abilities. Restorative practices, akin to restorative justice, build relationships, make connections, and foster a reader's sense of ownership and empowerment. What would happen if…

  20. Graded Readers: Validating Reading Levels across Publishers

    Science.gov (United States)

    Rodrigo, Victoria

    2016-01-01

    Graded readers can be an optimal resource to help language students improve and personalize their learning experience. An extensive reading library with graded readers and well-defined levels of reading difficulty increases language students' chances of having a successful reading experience and become independent readers. However, when it comes…

  1. A novel fluorescent sensor for measurement of CFTR function by flow cytometry.

    Science.gov (United States)

    Vijftigschild, Lodewijk A W; van der Ent, Cornelis K; Beekman, Jeffrey M

    2013-06-01

    Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene cause cystic fibrosis. CFTR-dependent iodide transport measured by fluorescent quenching of ectopically expressed halide-sensitive yellow fluorescent protein (YFP) is widely being used to study CFTR function by microscopy or plate readers. Since YFP fluorescence in these systems is dependent on YFP expression levels and iodide concentration, differences in sensor expression level between experimental units are normalized at the start of each experiment. To allow accurate measurement of CFTR function by flow cytometry, we reasoned that co-expression of an iodide insensitive fluorescent protein would allow for normalization of sensor expression levels and more accurate quantification of CFTR function. Our data indicated that dsRed and mKate fluorescence are iodide insensitive, and we determined an optimal format for co-expression of these fluorescent proteins with halide-sensitive YFP. We showed using microscopy that ratiometric measurement (YFP/mKate) corrects for differences in sensor expression levels. Ratiometric measurements were essential to accurately measure CFTR function by flow cytometry that we here describe for the first time. Mixing of wild type or mutant CFTR expressing cells indicated that addition of approximately 10% of wild type CFTR expressing cells could be distinguished by ratiometric YFP quenching. Flow cytometric ratiometric YFP quenching also allowed us to study CFTR mutants associated with differential residual function upon ectopic expression. Compared with conventional plate-bound CFTR function assays, the flow cytometric approach described here can be used to study CFTR function in suspension cells. It may be further adapted to study CFTR function in heterologous cell populations using cell surface markers and selection of cells that display high CFTR function by cell sorting.

  2. The Reader's Devices: The affordances of ebook readers

    Directory of Open Access Journals (Sweden)

    Heather MacFadyen

    2011-04-01

    Full Text Available Print books and ebook devices now co-exist in a reading eco-system. The ways in which readers understand and describe their experience of reading on ebook devices is shaped by long-established cultural expectations about the abstract as well as the physical affordances of the print book. Ebook devices cannot help but challenge those expectations. A review of readers’ reactions to the emergence of ebook devices offers a glimpse into the complex cultural position of both the idea and the experience of reading.

  3. A computerized track detector reader

    Energy Technology Data Exchange (ETDEWEB)

    Rosinski, S.W. (Centralne Lab. Ochrony Radiologicznej, Warsaw (Poland))

    1993-01-01

    The structure and basic operation function of a computerized facility named Track Detection Reader is described. This facility is used for recording, counting and evaluation of defects made by [alpha]-particles in a solid state detector. It consists of a microscope equipped with the movable stage, a TV screen and PC-AT computer. The microscope stage is being controlled by a stepper motor. The TV screen enables surface visualization of the detector analyzed while the PC-AT computer is being used for digital analysis of the detector surface, according to the functions of the program. (author). 4 refs, 3 figs.

  4. Variable Distance Angular Symbology Reader

    Science.gov (United States)

    Schramm, Harry F., Jr. (Inventor); Corder, Eric L. (Inventor)

    2006-01-01

    A variable distance angular symbology, reader utilizes at least one light source to direct light through a beam splitter and onto a target. A target may be angled relative to the impinging light beam up to and maybe even greater than 45deg. A reflected beam from the target passes through the beam splitter and is preferably directed 90deg relative to the light source through a telecentric lens to a scanner which records an image of the target such as a direct part marking code.

  5. Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen dye.

    Science.gov (United States)

    Moreno, Luis A; Cox, Kendra L

    2010-11-05

    Quantification of DNA, especially in small concentrations, is an important task with a wide range of biological applications including standard molecular biology assays such as synthesis and purification of DNA, diagnostic applications such as quantification of DNA amplification products, and detection of DNA molecules in drug preparations. During this video we will demonstrate the capability of the Hitachi F-7000 Fluorescence Spectrophotometer equipped with a Micro Plate Reader accessory to perform dsDNA quantification using Molecular Probes Quant-it PicoGreen dye reagent kit. The F-7000 Fluorescence Spectrophotometer offers high sensitivity and high speed measurements. It is a highly flexible system capable of measuring fluorescence, luminescence, and phosphorescence. Several measuring modes are available, including wavelength scan, time scan, photometry and 3-D scan measurement. The spectrophotometer has sensitivity in the range of 50 picomoles of fluorescein when using a 300 μL sample volume in the microplate, and is capable of measuring scan speeds of 60,000 nm/minute. It also has a wide dynamic range of up to 5 orders of magnitude which allows for the use of calibration curves over a wide range of concentrations. The optical system uses all reflective optics for maximum energy and sensitivity. The standard wavelength range is 200 to 750 nm, and can be extended to 900 nm when using one of the optional near infrared photomultipliers. The system allows optional temperature control for the plate reader from 5 to 60 degrees Celsius using an optional external temperature controlled liquid circulator. The microplate reader allows for the use of 96 well microplates, and the measuring speed for 96 wells is less than 60 seconds when using the kinetics mode. Software controls for the F-7000 and Microplate Reader are also highly flexible. Samples may be set in either column or row formats, and any combination of wells may be chosen for sample measurements. This allows

  6. Thousand-fold fluorescent signal amplification for mHealth diagnostics.

    Science.gov (United States)

    Balsam, Joshua; Rasooly, Reuven; Bruck, Hugh Alan; Rasooly, Avraham

    2014-01-15

    The low sensitivity of Mobile Health (mHealth) optical detectors, such as those found on mobile phones, is a limiting factor for many mHealth clinical applications. To improve sensitivity, we have combined two approaches for optical signal amplification: (1) a computational approach based on an image stacking algorithm to decrease the image noise and enhance weak signals, and (2) an optical signal amplifier utilizing a capillary tube array. These approaches were used in a detection system which includes multi-wavelength LEDs capable of exciting many fluorophores in multiple wavelengths, a mobile phone or a webcam as a detector, and capillary tube array configured with 36 capillary tubes for signal enhancement. The capillary array enables a ~100× increase in signal sensitivity for fluorescein, reducing the limit of detection (LOD) for mobile phones and webcams from 1000 nM to 10nM. Computational image stacking enables another ~10× increase in signal sensitivity, further reducing the LOD for webcam from 10nM to 1 nM. To demonstrate the feasibility of the device for the detection of disease-related biomarkers, adenovirus DNA labeled with SYBR green or fluorescein was analyzed by both our capillary array and a commercial plate reader. The LOD for the capillary array was 5 ug/mL, and that of the plate reader was 1 ug/mL. Similar results were obtained using DNA stained with fluorescein. The combination of the two signal amplification approaches enables a ~1000× increase in LOD for the webcam platform. This brings it into the range of a conventional plate reader while using a smaller sample volume (10 ul) than the plate reader requires (100 ul). This suggests that such a device could be suitable for biosensing applications where up to 10 fold smaller sample sizes are needed. The simple optical configuration for mHealth described in this paper employing the combined capillary and image processing signal amplification is capable of measuring weak fluorescent signals

  7. A Sign Language Screen Reader for Deaf

    Science.gov (United States)

    El Ghoul, Oussama; Jemni, Mohamed

    Screen reader technology has appeared first to allow blind and people with reading difficulties to use computer and to access to the digital information. Until now, this technology is exploited mainly to help blind community. During our work with deaf people, we noticed that a screen reader can facilitate the manipulation of computers and the reading of textual information. In this paper, we propose a novel screen reader dedicated to deaf. The output of the reader is a visual translation of the text to sign language. The screen reader is composed by two essential modules: the first one is designed to capture the activities of users (mouse and keyboard events). For this purpose, we adopted Microsoft MSAA application programming interfaces. The second module, which is in classical screen readers a text to speech engine (TTS), is replaced by a novel text to sign (TTSign) engine. This module converts text into sign language animation based on avatar technology.

  8. Pulse plating

    CERN Document Server

    Hansal, Wolfgang E G; Green, Todd; Leisner, Peter; Reichenbach, Andreas

    2012-01-01

    The electrodeposition of metals using pulsed current has achieved practical importance in recent years. Although it has long been known that changes in potential, with or without polarity reversal, can significantly affect the deposition process, the practical application of this has been slow to be adopted. This can largely be explained in terms of the complex relationship between the current regime and its effect on the electrodeposition process. In order to harness these effects, an understanding of the anodic and cathodic electrochemical processes is necessary, together with the effects of polarity reversal and the rate of such reversals. In this new monograph, the basics of metal electrodeposition from solution are laid out in great detail in seven distinct chapters. With this knowledge, the reader is able to predict how a given pulse train profile can be adopted to achieve a desired outcome. Equally important is the choice of a suitable rectifier and the ancillary control circuits to enable pulse platin...

  9. Reader Response Theory and Classroom Practices.

    Science.gov (United States)

    Bush, Harold K., Jr.

    1994-01-01

    Presents annotations of nine journal articles, monographs, and "learning packages" (published between 1989 and 1993) that examine teaching approaches founded upon the insights of reader response theory. (RS)

  10. Selective targeting of epigenetic reader domains.

    Science.gov (United States)

    Greschik, Holger; Schüle, Roland; Günther, Thomas

    2017-05-01

    Epigenetic regulators including writers, erasers, and readers of chromatin marks have been implicated in numerous diseases and are therefore subject of intense academic and pharmaceutical research. While several small-molecule inhibitors targeting writers or erasers are either approved drugs or are currently being evaluated in clinical trials, the targeting of epigenetic readers has lagged behind. Proof-of-principle that epigenetic readers are also relevant drug targets was provided by landmark discoveries of selective inhibitors targeting the BET family of acetyl-lysine readers. More recently, high affinity chemical probes for non-BET acetyl- and methyl-lysine reader domains have also been developed. Areas covered: This article covers recent advances with the identification and validation of inhibitors and chemical probes targeting epigenetic reader domains. Issues related to epigenetic reader druggability, quality requirements for chemical probes, interpretation of cellular action, unexpected cross-talk, and future challenges are also discussed. Expert opinion: Chemical probes provide a powerful means to unravel biological functions of epigenetic readers and evaluate their potential as drug targets. To yield meaningful results, potency, selectivity, and cellular target engagement of chemical probes need to be stringently validated. Future chemical probes will probably need to fulfil additional criteria such as strict target specificity or the targeting of readers within protein complexes.

  11. E-book Reader Devices and Libraries

    Directory of Open Access Journals (Sweden)

    Pažur, I.

    2011-03-01

    Full Text Available Most library studies thematically related to electronic books don't consider readers of electronic books. Only in recent years librarians conduct studies in which they want to find out readers' opinions about the possibilities, advantages and disadvantages of reading using e-readers, as well as their possible application in the libraries.User studies of e-readers have shown that their opinion is generally positive, but great attachment to traditional books is still present, e-readers are still seen only as an additional tool for reading. Sony with its e-reader (the latest Reader model Daily and Reader Store online bookstore (http://ebookstore.sony.com/is the only one who cooperate with libraries and has made lending electronic books possible. Cooperation was launched in 2009th,and the New York Public Library was the first library that offered such a service.Cooperation between Sony and libraries, indicates clearly what the near future could be if other online booksellers / publishers begin to follow the model of lending e-books through the libraries over the network. However it is possible that a large online bookstores / publishers consider that the further price reduction of e-readers and electronic books will constantly increase their sales, and in that case lending e-books will be unnecessary.Are the libraries ready for this scenario?

  12. [Readers' position against induced abortion].

    Science.gov (United States)

    1981-08-25

    Replies to the request by the Journal of Nursing on readers' positions against induced abortion indicate there is a definite personal position against induced abortion and the assistance in this procedure. Some writers expressed an emotional "no" against induced abortion. Many quoted arguments from the literature, such as a medical dictionary definition as "a premeditated criminally induced abortion." The largest group of writers quoted from the Bible, the tenor always being: "God made man, he made us with his hands; we have no right to make the decision." People with other philosophies also objected. Theosophical viewpoint considers reincarnation and the law of cause and effect (karma). This philosophy holds that induced abortion impedes the appearance of a reincarnated being. The fundamental question in the abortion problem is, "can the fetus be considered a human life?" The German anatomist Professor E. Bleckschmidt points out that from conception there is human life, hence the fertilized cell can only develop into a human being and is not merely a piece of tissue. Professional nursing interpretation is that nursing action directed towards killing of a human being (unborn child) is against the nature and the essence of the nursing profession. A different opinion states that a nurse cares for patients who have decided for the operation. The nurse doesn't judge but respects the individual's decision. Some proabortion viewpoints considered the endangering of the mother's life by the unborn child, and the case of rape. With the arguments against abortion the question arises how to help the woman with unwanted pregnancy. Psychological counseling is emphasized as well as responsible and careful assistance. Referral to the Society for Protection of the Unborn Child (VBOK) is considered as well as other agencies. Further reader comments on this subject are solicited.

  13. Fluorescence assessment of Lactococcus lactis viability

    NARCIS (Netherlands)

    Bunthof, C.J.; Braak, van den S.; Breeuwer, P.; Rombouts, F.M.; Abee, T.

    2000-01-01

    The reproduction and activity of lactic acid bacteria (LAB) are essential in their applications in the dairy industry and other fermentations. Traditionally used methods like plate counting and acidification tests require long incubation times and provide limited information. Fluorescence techniques

  14. Create Your Plate

    Medline Plus

    Full Text Available ... A A A Listen En Español Create Your Plate Create Your Plate is a simple and effective ... and that your options are endless. Create Your Plate! Click on the plate sections below to add ...

  15. Reader Profiles for Adults with Low Literacy Skills: A Quest to Find Resilient Readers

    Science.gov (United States)

    Binder, Katherine S.; Lee, Cheryl S.

    2012-01-01

    Resilient readers are those who, despite their poor phonological decoding skills, have good comprehension abilities (Jackson & Doellinger, 2002). Thus far, these readers have been identified in college settings. The purpose of this study was to a) determine if this reader profile was present in a sample taken from an Adult Basic Education…

  16. How Does a Reader Make a Poem Meaningful? Reader-Response Theory and the Poetry Portfolio.

    Science.gov (United States)

    Tompkins, Sandra Lee

    1997-01-01

    Describes how a reader-response approach can help students construct a portfolio of readings that reflects their development as poetry readers. Describes using a reader-response journal, communal learning activities, and a portfolio to create a recursive process through which students develop a better understanding of how poetry works. Discusses…

  17. Reader responses to literary depictions of rape

    NARCIS (Netherlands)

    E.M. Koopman (Emy); M. Hilscher (Michelle); G.C. Cupchik (Gerald)

    2012-01-01

    textabstractThis study explored reader responses to different literary depictions of rape. Four literary excerpts were used and divided as aesthetic versus nonaesthetic (style) and allusive versus explicit (detail). The general question was how readers would react to literary fragments depicting rap

  18. The active reader: what is active?

    NARCIS (Netherlands)

    Woerkum, van C.

    2012-01-01

    How writers can adapt to their readers is an important issue in effective communication strategies, and certainly crucial in the case of functional texts. Therefore, it is necessary to look at how readers are constructed as partners in a communication co-production. This article explores the concept

  19. Intermediate Amharic Cultural Reader. Final Report.

    Science.gov (United States)

    Leslau, Wolf

    This reader is intended to provide material for the intermediate-level student of Amharic, as well as to introduce the student to the cultural and social life of Ethiopia. The 39 texts were each prepared by a different student at Haile Selassie I University, thus providing the reader with a variety of language styles. The Amharic texts are…

  20. Advanced Hindi Reader in the Social Sciences.

    Science.gov (United States)

    Vatuk, Ved Prakash

    This reader contains 25 selections in standard Hindi by recognized authorities in the major fields of social science; namely sociology, anthropology, folklore, economics, and political science. The writings, evenly divided both in content and style, are intended to give the reader a broad perspective of Indian culture. A 128-page Hindi-English…

  1. Fiction Reading Strategies of College Readers

    Science.gov (United States)

    Poole, Alex

    2013-01-01

    Research has shown that skilled college readers use a variety of strategies in flexible ways in order to comprehend academic texts. However, little is known about how skilled college readers use strategies when reading fiction, in spite of the fact that literature courses are required at many universities and thousands of students regularly major…

  2. Using Nooks to Hook Reluctant Readers

    Science.gov (United States)

    Dierking, Rebecca

    2015-01-01

    This article presents the findings of a two-year qualitative study of electronic reading device use with high school sophomores, most of whom self-identified as reluctant or struggling readers. Electronic readers were used primarily in one weekly fifty-minute class period, during silent sustained reading, wherein students chose freely their texts.…

  3. Developing Preservice Teachers' Perspectives on Reader Response.

    Science.gov (United States)

    Grisham, Dana L.

    2001-01-01

    Examines preservice teachers' developing conceptions of reader response theory, specifically focusing on the importance of aesthetic response to students' engagement with and motivation for reading. Finds that the aesthetic reader stance predominated in students' written responses and discussions; and that written response did not influence the…

  4. An Overview of Reader-Response Theory

    Institute of Scientific and Technical Information of China (English)

    高扬

    2008-01-01

    This paper aims to give a brief introduction to reader-response theory.including its origin,main concepts and general classification. with the following conclusion drawn in the end: reader-response theory. in spite of its subjectivity,has provided a broader horizon for literary criticism due to its variety and openness.

  5. Developing Readers: Lessons from Agatha Christie.

    Science.gov (United States)

    Mackey, Margaret

    1991-01-01

    Reminds readers that looking at individual authors rather than dealing in generalizations is a very meaningful approach to increasing an understanding of how reading works by making sense of the text. Discusses how Agatha Christie's writing follows very predictable patterns and may provide useful experiences for novice readers. (MG)

  6. Reader Imposed Structure and Prose Retention.

    Science.gov (United States)

    Grabe, Mark D.

    1979-01-01

    Two experiments investigated the impact of a reader's perspective on prose learning: (1) subjects read stories from one of two directed perspectives or with no directed perspective; or (2) readers organized and familiarized themselves with a perspective before the perspective was applied to a story. Perspective influenced recall and organization.…

  7. Planning Behaviour in Good and Poor Readers

    Science.gov (United States)

    Mahapatra, Shamita

    2016-01-01

    A group of 50 good readers and a group of 50 poor readers of Grade 5 matched for age and intelligence and selected on the basis of their proficiency in reading comprehension were tested for their competence in word reading and the process of planning at three different levels, namely, perceptual, memory and conceptual in order to study the…

  8. Postmodern theories about readers in electronic environment

    Directory of Open Access Journals (Sweden)

    Ivanka Kuić

    2015-03-01

    Full Text Available Introductory part of the paper discusses theories about readers in the last decades of the 20th century. In particular, two big theoretical schools are discussed: aesthetic reception theory and reader-response-criticism movement. Readers are a subject of very different scientific disciplines: literature theory, sociology, anthropology, book history and library science. The idea that a reader is an essential subject for future life of a literary work is common to all theorists. By constructing the theory about a reader, theorists have thought about the reader who uses the conventions of printed text. The issue whether these concepts correspond to electronic surroundings is discussed. Characteristics of the hypertext are emphasized as a new paradigm, and also the issue weather readers enjoys in hypertextual fiction. In conclusion, paper expands the virtual dimension of Isers's theory about interaction between the text and the reader, and also Fish's concept of “interpretative community” which may be constituted on the Internet as a flexible virtual community.

  9. Reader-Centered Technical Writing

    Science.gov (United States)

    Narayanan, M.

    2012-12-01

    Technical writing is an essential part of professional communication and in recent years it has shifted from a genre-based approach. Formerly, technical writing primarily focused on generating templates of documents and sometimes it was creating or reproducing traditional forms with minor modifications and updates. Now, technical writing looks at the situations surrounding the need to write. This involves deep thinking about the goals and objectives of the project on hand. Furthermore, one observes that it is very important for any participatory process to have the full support of management. This support needs to be well understood and believed by employees. Professional writing may be very persuasive in some cases. When presented in the appropriate context, technical writing can persuade a company to improve work conditions ensuring employee safety and timely production. However, one must recognize that lot of professional writing still continues to make use of reports and instruction manuals. Normally, technical and professional writing addresses four aspects. Objective: The need for generating a given professionally written technical document and the goals the document is expected to achieve and accomplish. Clientele: The clientele who will utilize the technical document. This may include the people in the organization. This may also include "unintended readers." Customers: The population that may be affected by the content of the technical document generated. This includes the stakeholders who will be influenced. Environment: The background in which the document is created. Also, the nature of the situation that warranted the generation of the document. Swiss Psychologist Jean Piaget's view of Learning focuses on three aspects. The author likes to extend Jean Piaget's ideas to students, who are asked to prepare and submit Reader-Centered Technical Writing reports and exercises. Assimilation: Writers may benefit specifically, by assimilating a new object into

  10. Chemical Inhibitors of Epigenetic Methyllysine Reader Proteins.

    Science.gov (United States)

    Milosevich, Natalia; Hof, Fraser

    2016-03-22

    Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation, stress responses, oncogenesis, and other disease pathways. The recent emergence of a small number of chemical inhibitors for methyllysine reader proteins supports the viability of these proteins as targets for drug development. This article introduces the biochemistry and biology of methyllysine reader proteins, provides an overview of functions for those families of readers that have been targeted to date (MBT, PHD, tudor, and chromodomains), and reviews the development of synthetic agents that directly block their methyllysine reading functions.

  11. Fluorescence spectroscopy

    DEFF Research Database (Denmark)

    Bagatolli, Luis

    2016-01-01

    Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses...

  12. Readers of histone methylarginine marks.

    Science.gov (United States)

    Gayatri, Sitaram; Bedford, Mark T

    2014-08-01

    Arginine methylation is a common posttranslational modification (PTM) that alters roughly 0.5% of all arginine residues in the cells. There are three types of arginine methylation: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA). These three PTMs are enriched on RNA-binding proteins and on histones, and also impact signal transduction cascades. To date, over thirty arginine methylation sites have been cataloged on the different core histones. These modifications alter protein structure, impact interactions with DNA, and also generate docking sites for effector molecules. The primary "readers" of methylarginine marks are Tudor domain-containing proteins. The complete family of thirty-six Tudor domain-containing proteins has yet to be fully characterized, but at least ten bind methyllysine motifs and eight bind methylarginine motifs. In this review, we will highlight the biological roles of the Tudor domains that interact with arginine methylated motifs, and also address other types of interactions that are regulated by these particular PTMs. This article is part of a Special Issue entitled: Molecular mechanisms of histone modification function. Copyright © 2014 Elsevier B.V. All rights reserved.

  13. PressReader User Guide for Android eReader

    OpenAIRE

    2013-01-01

    Представлено краткое руководство для работы с электронными газетами и журналами на платформе Library PressDisplay на английском языке с помощью Android eReader. "Библиотека ПрессДисплей" - самый большой киоск ежедневной прессы, предлагает полные цифровые копии (больше контента, чем на Google) более 2300 газет и журналов из 100 стран на 54 языках и мнгновенный перевод с 13 языков....

  14. Testing a dual-fluorescence assay to monitor the viability of filamentous cyanobacteria.

    Science.gov (United States)

    Johnson, Tylor J; Hildreth, Michael B; Gu, Liping; Zhou, Ruanbao; Gibbons, William R

    2015-06-01

    Filamentous cyanobacteria are currently being engineered to produce long-chain organic compounds, including 3rd generation biofuels. Because of their filamentous morphology, standard methods to quantify viability (e.g., plate counts) are not possible. This study investigated a dual-fluorescence assay based upon the LIVE/DEAD® BacLight™ Bacterial Viability Kit to quantify the percent viability of filamentous cyanobacteria using a microplate reader in a high throughput 96-well plate format. The manufacturer's protocol calls for an optical density normalization step to equalize the numbers of viable and non-viable cells used to generate calibration curves. Unfortunately, the isopropanol treatment used to generate non-viable cells released a blue pigment that altered absorbance readings of the non-viable cell solution, resulting in an inaccurate calibration curve. Thus we omitted this optical density normalization step, and carefully divided cell cultures into two equal fractions before the isopropanol treatment. While the resulting calibration curves had relatively high correlation coefficients, their use in various experiments resulted in viability estimates ranging from below 0% to far above 100%. We traced this to the apparent inaccuracy of the propidium iodide (PI) dye that was to stain only non-viable cells. Through further analysis via microplate reader, as well as confocal and wide-field epi-fluorescence microscopy, we observed non-specific binding of PI in viable filamentous cyanobacteria. While PI will not work for filamentous cyanobacteria, it is possible that other fluorochrome dyes could be used to selectively stain non-viable cells. This will be essential in future studies for screening mutants and optimizing photobioreactor system performance for filamentous cyanobacteria.

  15. Normalization of Mendeley reader impact on the reader- and paper-side

    Energy Technology Data Exchange (ETDEWEB)

    Haunschild, R.; Bornmann, L.

    2016-07-01

    For the normalization of citation counts, two different kinds of methods are possible and used in bibliometrics: the cited-side and citing-side normalizations both of which can also be applied in the normalization of “Mendeley reader counts”. Recently, we introduced the paper-side normalization of reader counts (mean normalized reader score, MNRS) which is an adaptation of the cited-side normalization. Since the calculation of the MNRS needs further data besides data from Mendeley (a field-classification scheme, such as the Web of Science subject categories), we introduce here the reader-side normalization of reader counts which is an adaptation of the citing-side normalization and does not need further data from other sources. In this study, all articles and reviews of the Web of Science core collection with publication year 2012 (and a DOI) are used to normalize their Mendeley reader counts. The newly proposed indicator (mean discipline normalized reader score, MDNRS) is obtained, compared with the MNRS and bare reader counts, and studied empirically. We find that: (i) normalization of Mendeley reader counts is necessary, (ii) the MDNRS is able to normalize Mendeley reader counts in several disciplines, and (iii) the MNRS is able to normalize Mendeley reader counts in all disciplines. This generally favorable result for the MNRS in all disciplines lead to the recommendation to prefer the MNRS over the MDNRS – provided that the user has an external field-classification scheme at hand. (Author)

  16. Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding.

    Science.gov (United States)

    Branigan, Emma; Pliotas, Christos; Hagelueken, Gregor; Naismith, James H

    2013-11-01

    Cysteine is an extremely useful site for selective attachment of labels to proteins for many applications, including the study of protein structure in solution by electron paramagnetic resonance (EPR), fluorescence spectroscopy and medical imaging. The demand for quantitative data for these applications means that it is important to determine the extent of the cysteine labeling. The efficiency of labeling is sensitive to the 3D context of cysteine within the protein. Where the label or modification is not directly measurable by optical or magnetic spectroscopy, for example, in cysteine modification to dehydroalanine, assessing labeling efficiency is difficult. We describe a simple assay for determining the efficiency of modification of cysteine residues, which is based on an approach previously used to determine membrane protein stability. The assay involves a reaction between the thermally unfolded protein and a thiol-specific coumarin fluorophore that is only fluorescent upon conjugation with thiols. Monitoring fluorescence during thermal denaturation of the protein in the presence of the dye identifies the temperature at which the maximum fluorescence occurs; this temperature differs among proteins. Comparison of the fluorescence intensity at the identified temperature between modified, unmodified (positive control) and cysteine-less protein (negative control) allows for the quantification of free cysteine. We have quantified both site-directed spin labeling and dehydroalanine formation. The method relies on a commonly available fluorescence 96-well plate reader, which rapidly screens numerous samples within 1.5 h and uses <100 μg of material. The approach is robust for both soluble and detergent-solubilized membrane proteins.

  17. Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding

    Science.gov (United States)

    Hagelueken, Gregor; Naismith, James H

    2013-01-01

    Cysteine is an extremely useful site for selective attachment of labels to proteins for many applications, including the study of protein structure in solution by electron paramagnetic resonance (EPR), fluorescence spectroscopy and medical imaging. The demand for quantitative data for these applications means that it is important to determine the extent of the cysteine labeling. The efficiency of labeling is sensitive to the 3D context of cysteine within the protein. Where the label or modification is not directly measurable by optical or magnetic spectroscopy, for example, in cysteine modification to dehydroalanine, assessing labeling efficiency is difficult. We describe a simple assay for determining the efficiency of modification of cysteine residues, which is based on an approach previously used to determine membrane protein stability. The assay involves a reaction between the thermally unfolded protein and a thiol-specific coumarin fluorophore that is only fluorescent upon conjugation with thiols. Monitoring fluorescence during thermal denaturation of the protein in the presence of the dye identifies the temperature at which the maximum fluorescence occurs; this temperature differs among proteins. Comparison of the fluorescence intensity at the identified temperature between modified, unmodified (positive control) and cysteine-less protein (negative control) allows for the quantification of free cysteine. We have quantified both site-directed spin labeling and dehydroalanine formation. The method relies on a commonly available fluorescence 96-well plate reader, which rapidly screens numerous samples within 1.5 h and uses <100 μg of material. The approach is robust for both soluble and detergent-solubilized membrane proteins. PMID:24091556

  18. Detection of food-borne pathogens by nanoparticle technology coupled to a low-cost cell reader

    Science.gov (United States)

    Noiseux, Isabelle; Bouchard, Jean-Pierre; Gallant, Pascal; Bourqui, Pascal; Cao, Honghe; Vernon, Marci; Johnson, Roger; Chen, Shu; Mermut, Ozzy

    2010-02-01

    The detection, identification and quantification of pathogenic microorganisms at low cost are of great interest to the agro-food industry. We have developed a simple, rapid, sensitive, and specific method for detection of food-borne pathogens based on use of nanoparticles alongside a low cost fluorescence cell reader for the bioassay. The nanoparticles are coupled with antibodies that allow specific recognition of the targeted Listeria in either a liquid or food matrix. The bioconjugated nanoparticles (FNP) contain thousands of dye molecules enabling significant amplification of the fluorescent signal emitted from each bacterium. The developed fluorescence Cell Reader is an LED-based reader coupled with suitable optics and a camera that acquires high resolution images. The dedicated algorithm allowed the counting of each individual nanoparticles-fluorescent bacterial cells thus enabling highly sensitive reading. The system allows, within 1 hour, the recovery and counting of 104 to 108 cfu/mL of Listeria in pure culture. However, neither the Cell Reader nor the algorithm can differentiate between the FNPs specifically-bound to the target and the residual unbound FNPs limiting sensitivity of the system. Since FNPs are too small to be washed in the bioassay, a dual tagging approach was implemented to allow online optical separation of the fluorescent background caused by free FNPs.

  19. Kobo eReader for dummies

    CERN Document Server

    Sandler, Corey

    2010-01-01

    Here's how to use and love your Kobo eReader, the gadget dedicated to reading! Want to carry hundreds of books, magazines, and newspapers everywhere and not hurt your back? Load this e-book onto your Kobo eReader and we'll show you how! It's a quick-and-easy course in reading electronically, shopping for e-books, converting your own files for the eReader, getting the most from the device, and keeping your Kobo happy. Hello, Kobo - get acquainted with all the features and controls and learn how to charge the battery Go shopping - download e-books, find free books, an

  20. GryphSens: A Smartphone-Based Portable Diagnostic Reader for the Rapid Detection of Progesterone in Milk.

    Science.gov (United States)

    Jang, Hyunwook; Ahmed, Syed Rahin; Neethirajan, Suresh

    2017-05-10

    Enzyme-linked immunosorbent assay (ELISA) is a popular assay technique for the detection and quantification of various biological substances due its high sensitivity and specificity. More often, it requires large and expensive laboratory instruments, which makes it difficult to conduct when the tests must be performed quickly at the point-of-care (POC). To increase portability and ease of use, we propose a portable diagnostic system based on a Raspberry Pi imaging sensor for the rapid detection of progesterone in milk samples. We designed, assembled, and tested a standalone portable diagnostic reader and validated it for progesterone detection against a standard ELISA assay using a commercial plate reader. The portable POC device yielded consistent results, regardless of differences in the cameras and flashlights between various smartphone devices. An Android application was built to provide front-end access to users, control the diagnostic reader, and display and store the progesterone measurement on the smartphone. The diagnostic reader takes images of the samples, reads the pixel values, processes the results, and presents the results on the handheld device. The proposed POC reader can perform to superior levels of performance as a plate reader, while adding the desirable qualities of portability and ease of use.

  1. Fluorescent multiplex cell flow systems and methods

    KAUST Repository

    Merzaban, Jasmeen

    2017-06-01

    Systems and methods are provided for simultaneously assaying cell adhesion or cell rolling for multiple cell specimens. One embodiment provides a system for assaying adhesion or cell rolling of multiple cell specimens that includes a confocal imaging system containing a parallel plate flow chamber, a pump in fluid communication with the parallel plate flow chamber via a flow chamber inlet line and a cell suspension in fluid communication with the parallel plate flow chamber via a flow chamber outlet line. The system also includes a laser scanning system in electronic communication with the confocal imaging system, and a computer in communication with the confocal imaging system and laser scanning system. In certain embodiments, the laser scanning system emits multiple electromagnetic wavelengths simultaneously it cause multiple fluorescent labels having different excitation wavelength maximums to fluoresce. The system can simultaneously capture real-time fluorescence images from at least seven cell specimens in the parallel plate flow chamber.

  2. Turning Polite Guests into Executive Readers.

    Science.gov (United States)

    Gates, Dale D.

    1983-01-01

    Expands the usual notion of critical reading from just the judgments readers make about the text to include judgments they make about their "reading" of the text. Describes activities to develop "executive" metacomprehension skills. (HTH)

  3. Towards Quranic reader controlled by speech

    CERN Document Server

    Yekache, Yacine; Kouninef, Belkacem

    2012-01-01

    In this paper we describe the process of designing a task-oriented continuous speech recognition system for Arabic, based on CMU Sphinx4, to be used in the voice interface of Quranic reader. The concept of the Quranic reader controlled by speech is presented, the collection of the corpus and creation of acoustic model are described in detail taking into account a specificities of Arabic language and the desired application.

  4. Towards Quranic reader controlled by speech

    Directory of Open Access Journals (Sweden)

    Yacine Yekache

    2011-11-01

    Full Text Available In this paper we describe the process of designing a task-oriented continuous speech recognition system for Arabic, based on CMU Sphinx4, to be used in the voice interface of Quranic reader. The concept of the Quranic reader controlled by speech is presented, the collection of the corpus and creation of acoustic model are described in detail taking into account a specificities of Arabic language and the desired application.

  5. A Reader-Response Criticism towards "Trifles"

    Institute of Scientific and Technical Information of China (English)

    王莎莉

    2007-01-01

    Susan Glaspell's Trifles is considered one of the most important feminist works in last century.This play is about a murder mystery that explores gender relationships,power between the sexes,and the nature of truth.In this article we are going to examine and explain the relationship between the text and the reader in the process of reading with the view of reader-response criticism.

  6. The Study of Selecting Sample Detecting Position and Lead Plate Inner Material in Thin Film Method X-Ray Fluorescence Measurement%薄膜法X射线荧光测量中样品检测位置及防护铅板内衬材料的选择研究

    Institute of Scientific and Technical Information of China (English)

    甘婷婷; 张玉钧; 赵南京; 殷高方; 董欣欣; 王亚萍; 刘建国; 刘文清

    2015-01-01

    (1)以型号316不锈钢金属板为研究对象,对薄膜法X射线荧光光谱测量中,样品检测位置的选择进行研究,确定了最佳的样品检测位置为样品距离X射线管和探测器水平基线1 cm处,并且与X射线管和探测器水平基线成16°角度。(2)以工业环境空气重金属污染物Pb ,Cd ,Cr为主要分析对象,在有铅板防护情况下进行薄膜法X射线荧光光谱测量研究,发现X射线会穿透样品薄膜而继续激发防护铅板,使得滤膜背景光谱中有较强的铅谱线干扰,会对实际样品中铅元素的测量产生影响。在薄样和防护铅板之间加上一层隔离材料,可有效避免防护铅板中铅谱线对样品测量产生的干扰。(3)以型号316不锈钢、黄铜、铝材、紫铜和聚四氟乙烯几种硬质隔离材料作为铅板内衬材料进行选择研究,结果表明:紫铜的X射线荧光光谱中所含元素的谱线最少,谱图中没有出现重金属Cr ,Cd ,Pb的谱峰,并且能量较高部分靶材散射光谱强度较弱,对实际样品中重金属元素Cr ,Cd和Pb的测量不会产生干扰,作为铅板的内衬金属材料可以避免防护铅板中铅元素谱线的干扰,是最佳的薄膜法X射线荧光光谱分析中铅板的内衬金属材料。该研究为组装及搭建便携式大气及水体重金属X射线荧光光谱分析仪提供了重要的理论依据。%(1) In this paper type 316 stainless steel metal plate as the research object ,the selection of sample detecting position was studied when thin film method X-ray fluorescence measurement was conducted .The study showed that the optimal location for the sample detection was sample distance X-ray tube and detector baseline 1cm with the baseline into a 16°angle .(2) Heavy metal pollutants of Pb ,Cd and Cr in industrial ambient air as the main analysis object ,when thin film method X-ray fluorescence conducted with lead plate protection ,X

  7. Reader Response Theory in the High School English Classroom.

    Science.gov (United States)

    Shelton, Karen Yvonne

    A study examined the theory concerning reader response and the rationale and practice of reader response in the high school English curriculum. Formal experimental studies existed that explored reader response practices in the high school setting, but no formal studies existed on the questioning practices of potential reader response teachers. A…

  8. Examining Readers' Evaluations of Objectivity and Bias in News Discourse

    Science.gov (United States)

    Cramer, Peter; Eisenhart, Christopher

    2014-01-01

    Readers' objectivity and bias evaluations of news texts were investigated in order to better understand the process by which readers make these kinds of judgments and the evidence on which they base them. Readers were primed to evaluate news texts for objectivity and bias, and their selections and metacommentary were analyzed. Readers detected…

  9. E-Book Readers in Higher Education

    Directory of Open Access Journals (Sweden)

    Arthur N. Olsen

    2013-04-01

    Full Text Available This article reports on a research project at the University of Agder that has studied the use of e-readers as a tool for academic study. E-readers (Kindle DX and iPad were loaded with texts from required reading lists in five courses with 94 participating students. Initially, 87 students responded to the invitation to participate in a survey, but eventually 13 of these submissions had to be removed, as the degree of completion was not sufficient. The final response rate achieved was 79%. Students were in general positive to the use of e-readers but still show a preference for print on paper as the best medium for serious academic study. When reading books, 54% preferred print, 28% a combination of print and e-reader, and finally only 11% were satisfied solely using an e-reader. The iPad scored significantly better than the Kindle DX on tasks that required active interaction with the texts such as highlighting and note taking.

  10. The differential effects of position, ad and reader characteristics on readers' processing of newspaper ads

    NARCIS (Netherlands)

    Smit, E.G.; Neijens, P.C.; Heath, R.

    2013-01-01

    Building on previous research on the processing of newspaper ads, this comprehensive field study, with 26,556 newspaper readers and 290 unique advertisements, investigated the combined effects of position in the newspaper, ad characteristics and reader characteristics. The results show a

  11. "It's All Coming Together": An Encounter between Implied Reader and Actual Reader in the Australian Rainforest

    Science.gov (United States)

    Williams, Sandra J.

    2008-01-01

    In this paper I discuss how taking a particular literary theory--the implied reader--serves to offer a focus for the teacher's initial reading of a text and provides a formative assessment tool. Iser's Implied Reader theory is discussed, after which a picture book, "Where the Forest Meets the Sea" by Jeannie Baker, is analysed from this…

  12. The differential effects of position, ad and reader characteristics on readers' processing of newspaper ads

    NARCIS (Netherlands)

    Smit, E.G.; Neijens, P.C.; Heath, R.

    2013-01-01

    Building on previous research on the processing of newspaper ads, this comprehensive field study, with 26,556 newspaper readers and 290 unique advertisements, investigated the combined effects of position in the newspaper, ad characteristics and reader characteristics. The results show a differentia

  13. Processless offset printing plates

    Directory of Open Access Journals (Sweden)

    Sanja Mahović Poljaček

    2015-06-01

    Full Text Available With the implementation of platesetters in the offset printing plate making process, imaging of the printing plate became more stable and ensured increase of the printing plate quality. But as the chemical processing of the printing plates still highly influences the plate making process and the graphic reproduction workflow, development of printing plates that do not require chemical processing for offset printing technique has been one of the top interests in graphic technology in the last few years. The main reason for that came from the user experience, where majority of the problems with plate making process could be connected with the chemical processing of the printing plate. Furthermore, increased environmental standards lead to reducing of the chemicals used in the industrial processes. Considering these facts, different types of offset printing plates have been introduced to the market today. This paper presents some of the processless printing plates.

  14. Contrast Study of structuralism and reader- response criticism

    Institute of Scientific and Technical Information of China (English)

    吴建晓; 郑灵珍

    2012-01-01

    In literary criticism, both structuralism and reader-response criticism enjoy their positions. They share similarities in some ways, but obviously they are two different ways of literary criticism, with structuralism focusing on text and reader-response criti- cism focusing on readers. This essay attempts to contrast structuralism with reader-response criticism in their assumption on text's meaning and the reader's role in the process of reading a text.

  15. To Our Readers In the New Year

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    WITH the deepening of China’s reform and the expansion of the policy of opening to the world, we greet the year 1995. A new year means a chance for development and a new beginning for any person or career. The editorial department of Women of China sends its best wishes to all our readers. Women of China magazine has entered its 39th year since its establishment. With love and concern from our large readership, the magazine has made friends with readers from countries and regions all over the

  16. Passive microfluidic array card and reader

    Science.gov (United States)

    Dugan, Lawrence Christopher [Modesto, CA; Coleman, Matthew A [Oakland, CA

    2011-08-09

    A microfluidic array card and reader system for analyzing a sample. The microfluidic array card includes a sample loading section for loading the sample onto the microfluidic array card, a multiplicity of array windows, and a transport section or sections for transporting the sample from the sample loading section to the array windows. The microfluidic array card reader includes a housing, a receiving section for receiving the microfluidic array card, a viewing section, and a light source that directs light to the array window of the microfluidic array card and to the viewing section.

  17. Reader construction in interactive online journalism

    DEFF Research Database (Denmark)

    Hartley, Jannie Møller

    how online journalists are constructing their readers, seeking to explain how these views affect practices of journalism. Gans idea of the “constructed public” is re-visited, by analyzing the newsroom negotiations between one hand enabling the user participation and on the other hand their very...... be seen as journalistic positioning strategies and interactivity and mediated connectivity as both vital and challenging for the very field of journalism. This indicates that not only do the different kinds of interactivity seem to imply different kind of readers – they also seem to imply different ideals...

  18. Multi Spectral Fluorescence Imager (MSFI)

    Science.gov (United States)

    Caron, Allison

    2016-01-01

    Genetic transformation with in vivo reporter genes for fluorescent proteins can be performed on a variety of organisms to address fundamental biological questions. Model organisms that may utilize an ISS imager include unicellular organisms (Saccharomyces cerevisiae), plants (Arabidopsis thaliana), and invertebrates (Caenorhabditis elegans). The multispectral fluorescence imager (MSFI) will have the capability to accommodate 10 cm x 10 cm Petri plates, various sized multi-well culture plates, and other custom culture containers. Features will include programmable temperature and light cycles, ethylene scrubbing (less than 25 ppb), CO2 control (between 400 ppm and ISS-ambient levels in units of 100 ppm) and sufficient airflow to prevent condensation that would interfere with imaging.

  19. Readerly and Writerly "Letters from the Park."

    Science.gov (United States)

    Conde, Susana

    1993-01-01

    Discusses in depth the film "Cartas del parque" ("Letters from the Park"), the first of six films in the "Amores Dificiles" series. Notes that the film is pervaded by the traditional overdetermination of gender roles. Suggests that an intrusive and authoritative narrator makes of this both a "readerly" and a…

  20. Reader Response: Young Children Can Do That!

    Science.gov (United States)

    Lowe, Kaye; Johnston, Cammie

    2000-01-01

    Describes "reader response" teaching techniques to enhance young children's critical thinking skills, build a sound literacy foundation, and clarify the relationship between reading and writing. Provides examples of response logs/journal entries, aesthetic responses such as drawings and dramatic reenactments, and student discussions…

  1. Critical Literacy as Comprehension: Expanding Reader Response

    Science.gov (United States)

    McLaughlin, Maureen; De Voogd, Glenn

    2004-01-01

    This article presents the theoretical underpinnings of critical literacy and related principles. It also provides ideas for creating environments to promote reading from a critical stance, teaching strategies, sample classroom applications, and annotated lists of theme-related texts. When engaging in critical literacy, readers move beyond…

  2. Reader-Response and the Pathos Principle.

    Science.gov (United States)

    Johnson, Nan

    1988-01-01

    Reviews and equates theories of reader-response and rhetorical theories on audience response (the pathos principle). Concludes that the fundamental synonymity between them represents a significant bridge between analysis of literary texts and the dynamics of formal and social discourse and provides a theoretical foundation for teaching reading and…

  3. Author and reader in Instructions for use

    NARCIS (Netherlands)

    Steehouder, Michael F.

    1997-01-01

    Instructions for use should not be seen as merely instrumental-they should also persuade the reader to read the text and to act accordingly. Moreover, they should establish a positive image of the product and the manufacturer. In this paper, a collection of instructions for use is used to identify s

  4. The Emergent Reader's Working Kit of Stereotypes

    Science.gov (United States)

    Mackey, Margaret

    2013-01-01

    This article draws on a careful study of series fiction read in the 1950s to explore how stereotypes feature in the development of a young reader's competence in learning to process stories in print. Five categories of stereotype are teased out: "embodied stereotypes," understood through physical experience; "working stereotypes," discerned…

  5. Japanese-American Internment. A Historical Reader.

    Science.gov (United States)

    2000

    This Historical Reader on "Japanese American Internment" in World War II introduces students to key events and issues during the period through the voices of people with firsthand experienced. Source documents and illustrations are arranged in chronological order and/or thematic units that establish context. Each selection is followed by…

  6. Identification of N(6)-methyladenosine reader proteins.

    Science.gov (United States)

    Zhou, Katherine I; Liu, Nian; Pan, Tao

    2017-08-15

    The reversible N(6)-methyladenosine (m(6)A) modification of eukaryotic messenger RNAs (mRNAs) is a widespread regulatory mechanism that impacts every step in the mRNA life cycle. The effect of m(6)A on mRNA fate depends on the binding of "m(6)A reader" proteins - RNA binding proteins that specifically bind to RNAs containing m(6)A. Here, we describe an RNA pull-down method that can be used to identify novel m(6)A reader proteins starting from a known m(6)A-modified site in cellular or viral RNA. We further describe how a combination of immunoprecipitation-based sequencing methods can be used to identify m(6)A-modified sites bound by an m(6)A reader protein on a transcriptome-wide level. The discovery of new m(6)A reader proteins and their m(6)A-modified targets would provide further insight into the mechanisms and functions of m(6)A in the cell. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. Advanced Vietnamese: A Reader in Culture.

    Science.gov (United States)

    Liem, Nguyen Dang

    This fourth-year reader in Vietnamese presents a broad picture of Vietnamese culture through a selection of scholarly texts written by Vietnamese. The book seeks to introduce students to the culture of the Vietnamese and facilitate a transition from general reading matter to a more literary and technical style of writing. General chapter…

  8. Understanding Deaf Readers: An Interpretative Phenomenological Analysis

    Science.gov (United States)

    Kelstone, Aaron Weir

    2013-01-01

    The development of reading skills, beyond a functional level, is difficult for most deaf readers. Standardized testing demonstrates a median 4th grade reading level that remains consistent even after national norming of the Stanford Achievement test on the population of deaf school children. Deaf education continues to generate various educational…

  9. Towards a Culturally Situated Reader Response Theory

    Science.gov (United States)

    Brooks, Wanda; Browne, Susan

    2012-01-01

    This article describes a theory of how culture enables literary interpretations of texts. We begin with a brief overview of the reader response field. From there, we introduce the theory and provide illustrative participant data examples. These data examples illustrate the four cultural positions middle grade students in our research assumed when…

  10. Classic Readers Theatre for Young Adults.

    Science.gov (United States)

    Barchers, Suzanne I.; Kroll, Jennifer L.

    This book presents 16 original scripts that have been adapted from classic works of literature for use for readers theatre with young adults and ESL (English as a Second Language) students. Adaptations of the following works are included: "Little Women" (Louisa May Alcott); episodes from "Don Quixote" (Miguel de Cervantes; "The Necklace" (Guy de…

  11. Revisiting the Reader's Rudder: A Comprehension Strategy.

    Science.gov (United States)

    Fischer, Cynthia

    2003-01-01

    Presents the structured comprehension method, a strategy that facilitates literal, inferential, and critical reading comprehension for passive readers who can decode but not comprehend. Uses the method to illustrate how other areas of students' instruction (e.g., vocabulary enhancement through morphemic analysis, use of a phonogram approach to…

  12. Understanding readers' understanding theory to practice

    CERN Document Server

    Tierney, Robert J; Mitchell, Judy Nichols

    2013-01-01

    This collection features papers addressing current issues in reading comprehension from cognitive and linguistic perspectives. Organized into three sections, the volume investigates text considerations and reader-text interactions. Each paper presents a substantial and comprehensive review of theory and research related to cognition and reading comprehension.

  13. The Emergent Reader's Working Kit of Stereotypes

    Science.gov (United States)

    Mackey, Margaret

    2013-01-01

    This article draws on a careful study of series fiction read in the 1950s to explore how stereotypes feature in the development of a young reader's competence in learning to process stories in print. Five categories of stereotype are teased out: "embodied stereotypes," understood through physical experience; "working stereotypes," discerned…

  14. Vocabulary Instruction for Second Language Readers

    Science.gov (United States)

    Nisbet, Deanna L.

    2010-01-01

    Over the past 20 years, research has consistently affirmed the importance of explicit vocabulary instruction for adult learners of English as a second language (ESL). Given the significant vocabulary demands faced by adult second language readers, ESL teachers must carefully target their instruction for maximum impact and to foster meaningful…

  15. Print Readers' Perceptions of Various Advertising Formats.

    Science.gov (United States)

    Lamb, Charles W., Jr.; And Others

    1979-01-01

    The findings of a study of print readers' perceptions of the believability and interest of various advertising formats fail to support increasing either the frequency or specificity of comparative messages. The findings suggest that advertisers should consider the dimensions of intensity and directionality in their message development. (GT)

  16. Transcending Bias through Reader-Response Theory

    Science.gov (United States)

    Soublis, Theoni; Winkler, Erik

    2004-01-01

    The preservice teachers from all disciplines will be benefited if they incorporate reading in their classes according to Dr. Louise Rosenblatt's reader-response theory. A teacher's experience with her students while reading Chris Crutcher's "Staying Fat for Sarah Byanes" in the Secondary Content Area and a student's response on the novel are…

  17. Combining Phonics: Approaches for Problem Readers.

    Science.gov (United States)

    Gillis, M. K.

    1982-01-01

    A method of teaching phonics to primary grade disabled readers which combines analytic, synthetic, and linguistic methods is described. The method involves starting with sight words, starting phonics synthetically with consonants, teaching other consonants analytically, adapting linguistic methods for vowels, and encouraging the use of context.…

  18. Filling Empty Pockets: Remedial Readers Make Meaning.

    Science.gov (United States)

    Dionisio, Marie

    1989-01-01

    Describes the effect on junior high remedial readers of a reading workshop applying principles from Nancie Atwell's "In the Middle," including self-selection of adolescent literature books, silent reading, dialogue journal writing, and sharing work with others. Augments the workshop with "mini-lessons" on elements of reading…

  19. Shakespeare and Reader's Theatre: Fellow Traveling Companions

    Science.gov (United States)

    Ratliff, Gerald Lee

    2010-01-01

    Whether constructed on literary analysis models or inspired by conventional acting theories, Reader's Theatre performance techniques are an invaluable instructional tool available to teachers who want their students to see, hear and feel Shakespeare texts in classroom discussion and performance. These exercises are designed to promote both a…

  20. Fluorescence assay for evaluating microbicidal activity of hand antiseptics.

    Science.gov (United States)

    Lopez-Gigosos, Rosa M; Mariscal, Alberto; Mariscal-Lopez, Eloisa; Gutierrez-Bedmar, Mario; Fernandez, Joaquin

    2015-11-01

    We developed a fluorescent β-d-glucuronidase activity (BGA)-based assay for detecting and quantifying Escherichia coli in samples to assess the biocide efficacy of hand antiseptics. The fluorescence level is proportional to the number of viable E. coli organisms present. We compared our assay results to those of the E. coli plate count method specified by the European standard for testing hygienic hand rub disinfectant products (EN1500). The plate count method requires excessive handling and materials and is not valid if the number of organisms per plate is too low or high for counting in many of the samples. We optimized the fluorescent assay based on the cleavage of 4-methylumbelliferyl-β-d-glucuronide by adding 4-nitrophenyl-β-d-glucuronide, a nonfluorogenic BGA substrate, to induce glucuronidase activity and reduce assay time. Furthermore, our method can be automated and eliminates the need for multiple dilutions. Fluorescence was temporally monitored, and the time required to reach a specific value of fluorescence was correlated with the initial number of viable E. coli organisms on the samples. There was a positive correlation (P counts by plate count and fluorescence methods. Reported effects in fluorescent BGA were compared to the EN1500 plate count method with five hand disinfectants. We found our method more advantageous, because it was as sensitive as the EN1500 method, requires less time to complete, and is less expensive and less laborious than conventional plating techniques.

  1. Replication-Competent Influenza A and B Viruses Expressing a Fluorescent Dynamic Timer Protein for In Vitro and In Vivo Studies.

    Science.gov (United States)

    Breen, Michael; Nogales, Aitor; Baker, Steven F; Perez, Daniel R; Martínez-Sobrido, Luis

    2016-01-01

    Influenza A and B viruses (IAV and IBV, respectively) cause annual seasonal human respiratory disease epidemics. In addition, IAVs have been implicated in occasional pandemics with inordinate health and economic consequences. Studying influenza viruses in vitro or in vivo requires the use of laborious secondary methodologies to identify infected cells. To circumvent this requirement, replication-competent infectious influenza viruses expressing an easily traceable fluorescent reporter protein can be used. Timer is a fluorescent protein that undergoes a time-dependent color emission conversion from green to red. The rate of spectral change is independent of Timer protein concentration and can be used to chronologically measure the duration of its expression. Here, we describe the generation of replication-competent IAV and IBV where the viral non-structural protein 1 (NS1) was fused to the fluorescent dynamic Timer protein. Timer-expressing IAV and IBV displayed similar plaque phenotypes and growth kinetics to wild-type viruses in tissue culture. Within infected cells, Timer's spectral shift can be used to measure the rate and cell-to-cell spread of infection using fluorescent microscopy, plate readers, or flow cytometry. The progression of Timer-expressing IAV infection was also evaluated in a mouse model, demonstrating the feasibility to characterize IAV cell-to-cell infections in vivo. By providing the ability to chronologically track viral spread, Timer-expressing influenza viruses are an excellent option to evaluate the in vitro and in vivo dynamics of viral infection.

  2. Replication-Competent Influenza A and B Viruses Expressing a Fluorescent Dynamic Timer Protein for In Vitro and In Vivo Studies.

    Directory of Open Access Journals (Sweden)

    Michael Breen

    Full Text Available Influenza A and B viruses (IAV and IBV, respectively cause annual seasonal human respiratory disease epidemics. In addition, IAVs have been implicated in occasional pandemics with inordinate health and economic consequences. Studying influenza viruses in vitro or in vivo requires the use of laborious secondary methodologies to identify infected cells. To circumvent this requirement, replication-competent infectious influenza viruses expressing an easily traceable fluorescent reporter protein can be used. Timer is a fluorescent protein that undergoes a time-dependent color emission conversion from green to red. The rate of spectral change is independent of Timer protein concentration and can be used to chronologically measure the duration of its expression. Here, we describe the generation of replication-competent IAV and IBV where the viral non-structural protein 1 (NS1 was fused to the fluorescent dynamic Timer protein. Timer-expressing IAV and IBV displayed similar plaque phenotypes and growth kinetics to wild-type viruses in tissue culture. Within infected cells, Timer's spectral shift can be used to measure the rate and cell-to-cell spread of infection using fluorescent microscopy, plate readers, or flow cytometry. The progression of Timer-expressing IAV infection was also evaluated in a mouse model, demonstrating the feasibility to characterize IAV cell-to-cell infections in vivo. By providing the ability to chronologically track viral spread, Timer-expressing influenza viruses are an excellent option to evaluate the in vitro and in vivo dynamics of viral infection.

  3. Determining the critical micelle concentration of a novel lipid-lowering agent, disodium ascorbyl phytostanyl phosphate (FM-VP4), using a fluorescence depolarization procedure.

    Science.gov (United States)

    Wasan, Kishor M; Choo, Eugene; Sivak, Olena; Wallis, Simon; Letchford, Kevin; Burt, Helen M; Stewart, David J; Lukic, Tatjana

    2004-08-01

    The objective of this study was to determine the critical micelle concentration (CMC) of a novel water-soluble plant sterol derivative (FM-VP4) using a fluorescence depolarization method. The CMC was determined by 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence depolarization. Test solutions of various concentrations of sodium dodecylsulphate (SDS) as a positive control or FM-VP4 in water were spiked with 2 microL of 4 mM DPH in tetrahydrofuran (THF) and left overnight to equilibrate in a dark chamber. Fluorescence of each solution was measured at room temperature using a Perseptive Biosystems Cytofluor Series 4000 multi-well plate reader. Fluorescence intensity increases as DPH is incorporated into the hydrophobic core of micelles. Thus, the CMC is the value at which an abrupt increase in intensity is observed. These points were observed at 8 mM and 0.014 mM for SDS and FM-VP4, respectively. Sodium dodecylsulphate was used as a positive control and supports the validity of our results, as the literature values of SDS are reported to be between 8-8.3 mM. The CMC of FM-VP4 is reported to be 0.014 mM.

  4. Imaging plates calibration to X-rays

    Science.gov (United States)

    Curcio, A.; Andreoli, P.; Cipriani, M.; Claps, G.; Consoli, F.; Cristofari, G.; De Angelis, R.; Giulietti, D.; Ingenito, F.; Pacella, D.

    2016-05-01

    The growing interest for the Imaging Plates, due to their high sensitivity range and versatility, has induced, in the last years, to detailed characterizations of their response function in different energy ranges and kind of radiation/particles. A calibration of the Imaging Plates BAS-MS, BAS-SR, BAS-TR has been performed at the ENEA-Frascati labs by exploiting the X-ray fluorescence of different targets (Ca, Cu, Pb, Mo, I, Ta) and the radioactivity of a BaCs source, in order to cover the X-ray range between few keV to 80 keV.

  5. Create Your Plate

    Medline Plus

    Full Text Available ... steps to get started: Using your dinner plate, put a line down the middle of the plate. ... vegetables . Now in one of the small sections, put grains and starchy foods. See this list of ...

  6. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate It's simple and effective for both managing diabetes and losing weight. Creating your plate lets ... 2016 Articles from Diabetes Forecast® magazine: wcie-meal-planning, In this section Food Planning Meals Diabetes Meal ...

  7. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook with Heart-Healthy Foods Holiday Meal ... Healthy Diet Create Your Plate Meal Planning for Vegetarian Diets Gluten Free Diets Holiday Meal Planning Cook ...

  8. Food guide plate

    Science.gov (United States)

    ... chips or cookies. VEGETABLES: MAKE HALF OF YOUR PLATE FRUITS AND VEGETABLES Vegetables can be raw, fresh, ... as a snack. FRUITS: MAKE HALF OF YOUR PLATE FRUITS AND VEGETABLES Fruits can be fresh, canned, ...

  9. Growth Plate Fractures

    Science.gov (United States)

    ... the most widely used by doctors is the Salter-Harris system, described below. Type I Fractures These ... incidence of growth plate fractures peaks in adolescence. Salter-Harris classification of growth plate fractures. AAOS does ...

  10. Create Your Plate

    Medline Plus

    Full Text Available ... Planning Meals Diabetes Meal Plans Create Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook ... Create Your Plate Meal Planning for Vegetarian Diets Gluten Free Diets Holiday Meal Planning Cook with Heart- ...

  11. Create Your Plate

    Medline Plus

    Full Text Available ... Planning Meals Diabetes Meal Plans Create Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook ... Create Your Plate Meal Planning for Vegetarian Diets Gluten Free Diets Holiday Meal Planning Cook with Heart- ...

  12. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate It's simple and effective for both managing diabetes and losing weight. Creating your plate lets you still choose the foods you want, but changes the portion sizes so you are getting larger ...

  13. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate It's simple and effective for both managing diabetes and losing weight. Creating your plate lets ... Sleeve Custom jerseys for your Tour de Cure team benefits the cause. Ask the Experts: Learn to ...

  14. Metacognition and reading: Comparing three forms of metacognition in normally developing readers and readers with dyslexia

    OpenAIRE

    Furnes, Bjarte Reidar; Norman, Elisabeth

    2015-01-01

    Metacognition refers to ‘cognition about cognition’ and includes metacognitive knowledge, strategies and experiences (Efklides, 2008; Flavell, 1979). Research on reading has shown that better readers demonstrate more metacognitive knowledge than poor readers (Baker & Beall, 2009), and that reading ability improves through strategy instruction (Gersten, Fuchs, Williams, & Baker, 2001). The current study is the first to specifically compare the three forms of metacognition in dyslexic (N = 22) ...

  15. Screening system of blocking agents of the receptor for advanced glycation endproducts in cells using fluorescence.

    Science.gov (United States)

    Jung, Dong Ho; Kim, Young Sook; Kim, Jin Sook

    2012-01-01

    Activation of the receptor for advanced glycation endproducts (RAGE) triggers cellular responses implicated in the pathogenesis of diabetic complications; blockade of RAGE has been shown to inhibit the development of diabetic complications. To develop a screening system to identify novel disruptors of advanced glycation endproducts (AGE)-RAGE binding, we used an AGE-RAGE binding system in RAGE-overexpressing cells; test compounds were screened using this system. To construct human RAGE-overexpressing cells, mouse mesangial cells (MMCs) were stably transfected with the pcDNA-human RAGE (hRAGE) vector and selected under 1 mg/mL gentamicin (G418). RAGE expression in hRAGE-overexpressing MMCs was analyzed by Western blotting with specific RAGE antibody. To identify novel disruptors of AGE-RAGE binding, 50 single compounds and AGE-bovine serum albumin (BSA)-Alexa 488 (AGE-BSA labeled with Alexa 488) were treated to the hRAGE-overexpressing MMCs. Nonbinding AGE-BSA-Alexa 488 was washed and fluorescence measured by microtiter plate reader (excitation wavelength, 485 nm; emission wavelength, 528 nm). In hRAGE-overexpressing cells, only treatment with AGE-BSA-Alexa 488 significantly increased fluorescence intensity in a dose-dependent manner. Of 50 compounds tested, genistein disrupted AGE-RAGE binding in a dose-dependent manner. This AGE-RAGE binding system using AGE-BSA-Alexa 488 in hRAGE-overexpressing cells was suitable for screening of agents that disrupt AGE-hRAGE binding.

  16. Validation of a Fluorescence Sensor Microtiterplate for Biogenic Amines in Meat and Cheese.

    Science.gov (United States)

    Khairy, Gasser M; Azab, Hassan A; El-Korashy, Sabry A; Steiner, Mark-Steven; Duerkop, Axel

    2016-09-01

    An optical sensor microtiterplate for quantitative analysis of the total content of biogenic amines (TAC) in meat and cheese was developed and validated for the first time. In the plate, a chameleon dye (Py-1) is embedded in a polymeric cocktail which is deposited on the bottom of the wells in a common microtiterplate. On reaction with biogenic amines (BAs), the fluorescence of Py-1 at 620 nm rapidly delivers a precise TAC. After 10 min incubation at 25 °C the determination of the TAC in various (real) samples is possible in high-throughput with a standard microplate reader. The optimized fluorescence method was validated for linearity, sensitivity, accuracy, precision (intraday and inter day repeatability) and recovery using histamine (HIS) as a representative BA. The sensor microtiterplate was successfully applied to quantitatively analyze the TAC in 10 real samples of cheese and meat obtained from various Egyptian markets. The TAC of these real samples obtained by the sensor microtiterplate was validated against the contents of BAs obtained by GC-MS at various times of storage. The data of the sensor microtiterplate agreed well with those of GC-MS. This demonstrates that the sensor microtiterplate is a reliable screening tool for the degradation status of food samples.

  17. Accelerated plate tectonics.

    Science.gov (United States)

    Anderson, D L

    1975-03-21

    The concept of a stressed elastic lithospheric plate riding on a viscous asthenosphere is used to calculate the recurrence interval of great earthquakes at convergent plate boundaries, the separation of decoupling and lithospheric earthquakes, and the migration pattern of large earthquakes along an arc. It is proposed that plate motions accelerate after great decoupling earthquakes and that most of the observed plate motions occur during short periods of time, separated by periods of relative quiescence.

  18. Create Your Plate

    Medline Plus

    Full Text Available ... Reset Plate Share Create Your Plate ! Share: Seven Simple Steps to Create Your Plate It's simple and effective for both managing diabetes and losing ... en.html Have Type 2 Diabetes? Our free program will help you live well. More from diabetes. ...

  19. Obliquity along plate boundaries

    Science.gov (United States)

    Philippon, Mélody; Corti, Giacomo

    2016-12-01

    Most of the plate boundaries are activated obliquely with respect to the direction of far field stresses, as roughly only 8% of the plate boundaries total length shows a very low obliquity (ranging from 0 to 10°, sub-orthogonal to the plate displacement). The obliquity along plate boundaries is controlled by (i) lateral rheological variations within the lithosphere and (ii) consistency with the global plate circuit. Indeed, plate tectonics and magmatism drive rheological changes within the lithosphere and consequently influence strain localization. Geodynamical evolution controls large-scale mantle convection and plate formation, consumption, and re-organization, thus triggering plate kinematics variations, and the adjustment and re-orientation of far field stresses. These geological processes may thus result in plate boundaries that are not perpendicular but oblique to the direction of far field stresses. This paper reviews the global patterns of obliquity along plate boundaries. Using GPlate, we provide a statistical analysis of present-day obliquity along plate boundaries. Within this framework, by comparing natural examples and geological models, we discuss deformation patterns and kinematics recorded along oblique plate boundaries.

  20. Fluorescence Assay for Evaluating Microbicidal Activity of Hand Antiseptics

    OpenAIRE

    Lopez-Gigosos, Rosa M.; Mariscal, Alberto; Mariscal-Lopez, Eloisa; Gutierrez-Bedmar, Mario; Fernandez, Joaquin

    2015-01-01

    We developed a fluorescent β-d-glucuronidase activity (BGA)-based assay for detecting and quantifying Escherichia coli in samples to assess the biocide efficacy of hand antiseptics. The fluorescence level is proportional to the number of viable E. coli organisms present. We compared our assay results to those of the E. coli plate count method specified by the European standard for testing hygienic hand rub disinfectant products (EN1500). The plate count method requires excessive handling and ...

  1. Using Reader Response in a College Literature Class.

    Science.gov (United States)

    Soles, Derek

    1995-01-01

    Claims that the insights of reader response theory can be brought into the teaching of poetry in college literature courses. Outlines methods for utilizing reader response techniques to help students enjoy and understand poetry. (HB)

  2. 21 CFR 866.2850 - Automated zone reader.

    Science.gov (United States)

    2010-04-01

    ...) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2850 Automated zone reader. (a) Identification. An automated zone reader is a mechanical device intended for medical purposes...

  3. A Problem Solving Framework for Managing Poor Readers in Classrooms.

    Science.gov (United States)

    Beck, Judith S.

    1988-01-01

    Points out that poor readers may exhibit behavioral, cognitive, and emotional problems. Offers a problem-solving framework for intervention in poor readers' nonacademic problems, and describes several possible types of intervention. (ARH)

  4. Fluorescent microspheres

    Science.gov (United States)

    Rembaum, A.

    1978-01-01

    Latex particles with attached antibodies have potential biochemical and environmental applications. Human red blood cells and lymphocytes have been labeled with fluorescent microspheres by either direct or indirect immunological technique. Immunolatex spheres can also be used for detecting and localizing specific cell surface receptors. Hormones and toxins may also be bondable.

  5. RFID reader immunity test against electrostatic discharge

    Directory of Open Access Journals (Sweden)

    Pospisilik Martin

    2016-01-01

    Full Text Available This paper provides a description of an immunity test against the electrostatic discharge according to the standard EN 61000-4-2 that was applied to an RFID reader. The RFID reader was primarily developed for access systems, employing the on-board recognition of the RFID tags. The results obtained by the test are described hereby as well as the discussion on the security of this solution. The results of this experiment are beneficial for the developers of RFID devices, as these devices are endangered by the electrostatic discharge brought by their users. The hereby described results also shown a security hole in a simple access system based on the RFID technology. Details can be found in the paper.

  6. Blind Braille readers mislocate tactile stimuli.

    Science.gov (United States)

    Sterr, Annette; Green, Lisa; Elbert, Thomas

    2003-05-01

    In a previous experiment, we observed that blind Braille readers produce errors when asked to identify on which finger of one hand a light tactile stimulus had occurred. With the present study, we aimed to specify the characteristics of this perceptual error in blind and sighted participants. The experiment confirmed that blind Braille readers mislocalised tactile stimuli more often than sighted controls, and that the localisation errors occurred significantly more often at the right reading hand than at the non-reading hand. Most importantly, we discovered that the reading fingers showed the smallest error frequency, but the highest rate of stimulus attribution. The dissociation of perceiving and locating tactile stimuli in the blind suggests altered tactile information processing. Neuroplasticity, changes in tactile attention mechanisms as well as the idea that blind persons may employ different strategies for tactile exploration and object localisation are discussed as possible explanations for the results obtained.

  7. Reader construction in interactive online journalism

    DEFF Research Database (Denmark)

    Hartley, Jannie Møller

    Studies suggest that online news journalists are very sceptical towards the value of the user participation, because of what they perceive as poor quality of the public participation, the scepticism varies from one interactivity feature to another and from newsroom to newsroom. This paper examines...... how online journalists are constructing their readers, seeking to explain how these views affect practices of journalism. Gans idea of the “constructed public” is re-visited, by analyzing the newsroom negotiations between one hand enabling the user participation and on the other hand their very...... be seen as journalistic positioning strategies and interactivity and mediated connectivity as both vital and challenging for the very field of journalism. This indicates that not only do the different kinds of interactivity seem to imply different kind of readers – they also seem to imply different ideals...

  8. Developing the Reader Self-Efficacy Scale

    Directory of Open Access Journals (Sweden)

    Hakan ÜLPER

    2013-04-01

    Full Text Available Reading comprehension has affective and social dimensions as well as its cognitive dimension. Thus, to understand and improve reading instruction, affective and social dimensions should be well understood. Perceived self-efficacy is one of affective issues influencing learning and it should be taken into consideration in reading instruction. With this in mind, this study aims at developing the Reader Self-Efficacy Scale with a group of 518 students as participants. Exploratory and confirmatory factor analyses revealed a 6-item scale with one factor. The Cronbach Alpha coefficient was determined to be 0.948. This new Reader Self-Efficacy Scale could easily be used for training and research purposes depending on its high validity and reliability.

  9. Angular shear plate

    Science.gov (United States)

    Ruda, Mitchell C [Tucson, AZ; Greynolds, Alan W [Tucson, AZ; Stuhlinger, Tilman W [Tucson, AZ

    2009-07-14

    One or more disc-shaped angular shear plates each include a region thereon having a thickness that varies with a nonlinear function. For the case of two such shear plates, they are positioned in a facing relationship and rotated relative to each other. Light passing through the variable thickness regions in the angular plates is refracted. By properly timing the relative rotation of the plates and by the use of an appropriate polynomial function for the thickness of the shear plate, light passing therethrough can be focused at variable positions.

  10. Plastic Logic quits e-reader market

    Science.gov (United States)

    Perks, Simon

    2012-07-01

    A UK firm spun out from the University of Cambridge that sought to be a world leader in flexible organic electronic circuits and displays has pulled out of the competitive e-reader market as it struggles to find a commercial outlet for its technology. Plastic Logic announced in May that it is to close its development facility in Mountain View, California, with the loss of around 40 jobs.

  11. Formalist criticism and reader-response theory

    OpenAIRE

    MOHAMMAD AL FUADI

    2014-01-01

    The article deals with the interaction of formalism as a trend in language and literature studies, on the one hand, and a teaching method, a technique of teaching to understand and investigate literary text proceeding from its structure and content, on the other hand. One of the main principles is dialogism (M. Bakhtin) that creates the coeducation between the writer and reader. Thus the process becomes bilateral, or even multilateral and it includes criticism on both parts a teacher and a st...

  12. The StorySpinner Sculptural Reader

    OpenAIRE

    Hooper, Clare; Weal, Mark

    2005-01-01

    This demo is of a hypertext reading system called StorySpinner. It follows the sculptural hypertext methodology and has been used as a test bed for experimenting with the authoring of narrative flow in automatically generated stories. Readers are able to select and read one of two available stories. Reading a story involves selecting tarot cards which are mapped to chunks of story text based on possible interpretations of the cards and information concerning current story state.

  13. Wikipedia Reader's Guide: The Missing Manual

    CERN Document Server

    Broughton, John

    2008-01-01

    Wikipedia Reader's Guide: The Missing Manual gives you the essential tools for getting the most out of Wikipedia. As a supplement to Wikipedia: The Missing Manual, this handbook provides a basic road map to the largest online collaborative encyclopedia. You'll learn the best ways to search Wikipedia for the information you need, how to navigate the encyclopedia by category, and what to do if you spot an error in an article.

  14. E-readers and visual fatigue.

    Directory of Open Access Journals (Sweden)

    Simone Benedetto

    Full Text Available The mass digitization of books is changing the way information is created, disseminated and displayed. Electronic book readers (e-readers generally refer to two main display technologies: the electronic ink (E-ink and the liquid crystal display (LCD. Both technologies have advantages and disadvantages, but the question whether one or the other triggers less visual fatigue is still open. The aim of the present research was to study the effects of the display technology on visual fatigue. To this end, participants performed a longitudinal study in which two last generation e-readers (LCD, E-ink and paper book were tested in three different prolonged reading sessions separated by--on average--ten days. Results from both objective (Blinks per second and subjective (Visual Fatigue Scale measures suggested that reading on the LCD (Kindle Fire HD triggers higher visual fatigue with respect to both the E-ink (Kindle Paperwhite and the paper book. The absence of differences between E-ink and paper suggests that, concerning visual fatigue, the E-ink is indeed very similar to the paper.

  15. A Comparison of Laterality Between Normal and Dyslexic Readers.

    Science.gov (United States)

    Keefe, Barbara

    Examined with 38 right-handed boys who were either dyslexic or normal readers and matched for age and IQ (mean age both groups=10.6, mean IQ normal readers=106, mean IQ dyslexic readers=105) were the weak, strong, and equal lateralization theories of dyslexia. Cerebral lateralization was measured for linguistic material (digits) using the dichotic…

  16. 21 CFR 886.5800 - Ophthalmic bar reader.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Ophthalmic bar reader. 886.5800 Section 886.5800...) MEDICAL DEVICES OPHTHALMIC DEVICES Therapeutic Devices § 886.5800 Ophthalmic bar reader. (a) Identification. An ophthalmic bar reader is a device that consists of a magnifying lens intended for use by a...

  17. Are You a Reader? 5th Graders Respond

    Science.gov (United States)

    Barone, Diane; Barone, Rebecca

    2016-01-01

    The author tells the story of a 5th-grade teacher who challenges her class to take on self-identities as readers. Students defined seven characteristics of what it means to be a good reader and considered whether those characteristics applied to them: Good readers read for fun, talk about books, usually finish the book they're reading, can relate…

  18. 21 CFR 886.5810 - Ophthalmic prism reader.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Ophthalmic prism reader. 886.5810 Section 886.5810...) MEDICAL DEVICES OPHTHALMIC DEVICES Therapeutic Devices § 886.5810 Ophthalmic prism reader. (a) Identification. An ophthalmic prism reader is a device intended for use by a patient who is in a supine position...

  19. Gifted Male Readers: Current Understandings and Suggestions for Future Research

    Science.gov (United States)

    Pagnani, Alexander R.

    2013-01-01

    Research literature concerning gifted male readers relies primarily on more extensive bodies of work regarding gifted males and male readers. Studied as a whole, the two halves portray a worrisome state of affairs for gifted male readers, who lag behind their female counterparts in the same patterns found across the ability spectrum. This literacy…

  20. Reader Engagement in English and Persian Applied Linguistics Articles

    Science.gov (United States)

    Ansarin, Ali Akbar; Tarlani-Aliabdi, Hassan

    2011-01-01

    There is an increasing interest in the way academic writers establish the presence of their readers over the past few years. Establishing the presence of readers or what Kroll (1984, p.181) calls imagining "a second voice" is accomplished when a writer refers "explicitly" to their readers using explicit linguistic resources…

  1. A Framework for Analyzing Reader-Text Interactions.

    Science.gov (United States)

    MacLean, Margaret

    1986-01-01

    This article presents a framework for analyzing verbal report data, attempting to integrate Fillmore's levels of envisionment and Galda's differentiation between reader and text-centered responses. It examines the extent to which readers rely on text based, reader-based, or interactive processes to understand text. Case study data is presented.…

  2. Optimization of a Yellow fluorescent protein-based iodide influx high-throughput screening assay for cystic fibrosis transmembrane conductance regulator (CFTR) modulators.

    Science.gov (United States)

    Sui, Jinliang; Cotard, Shakira; Andersen, Jennifer; Zhu, Ping; Staunton, Jane; Lee, Margaret; Lin, Stephen

    2010-12-01

    Cystic fibrosis is an inherited, life-threatening disease associated with mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. The most common mutation, F508del CFTR, is found in 90% of CF patients. The loss of a single amino acid (phenylalanine at position 508) results in malformed CFTR with defective trafficking to the plasma membrane and impaired channel function. A functional assay with cells expressing F508del CFTR has been previously described by others using genetically engineered halide-sensitive yellow fluorescent protein to screen for CFTR modulators. We adapted this yellow fluorescent protein assay to 384-well plate format with a high-throughput screening plate reader, and optimized the assay in terms of data quality, resolution, and throughput, with target-specific protocols. The optimized assay was validated with reference compounds from cystic fibrosis foundation therapeutics. On the basis of the Z-factor range (≥0.5) and the potential productivity, this assay is well suited for high-throughput screening. It was successfully used to screen for active single agent and synergistic combinations of single agent modulators of F508del CFTR from a library collection of current active pharmaceutical ingredients (supported by Cystic Fibrosis Foundation Therapeutics).

  3. Sensitivity of detection of bacteria with fluorescent and luminescent phenotypes using different instruments

    Science.gov (United States)

    Brovko, Lubov Y.; Griffiths, Mansel W.

    2000-04-01

    The problem of bacterial enumeration in different samples is of great importance in many fields of research. Construction of recombinant fluorescent and luminescent bacteria that can be easily detected by nondestructive instrumental methods proves us with an opportunity to monitor bacteria in a wide variety of clinical, environmental and food samples in real time. Three different labels were employed: Green Fluorescent Protein (GFP), Bacterial luciferase (BL) and Firefly Luciferase (FFL). Both plasmid and chromosomal transformants of different strains of E. coli, P. putida and S. enteritidis were used. For the detection of the in vivo GFP the Shimadzu RF 540 spectrofluorimeter, Labsystems FL- 500 plate fluorimeter and Night Owl LB 98 CCD-camera from EG and G Berthold supplied with excitation light source and proper spectral filters both in macroscopic and microscopic mode were used. For the detection of in vivo luminescence of BL and FFL, tube luminometer BG-P from GEM Biomedical Inc., luminometric plate reader from BioOrbit, BIQ Bioview CCD camera from Cambridge Imaging Ltd and Night Owl LB 98 CCD camera both in macroscopic and microscopic mode were used. The expression levels of the labels, their stability, stability of the signal and detection limits of tagged bacteria were investigated. The detection limits for GFP tagged bacteria were 5 X 104 - 6 X 106, for BL tagged bacteria 5 X 102 - 2 X 105, and for FFL tagged bacteria - 4 X 103 - 106 CFU/ml, depending on the instrument used. Single bacteria could be detected with the help of the Night Owl in the microscopic mode.

  4. How to diagnose any type of TLD Reader?; Como diagnosticar cualquier tipo de TLD Reader?

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez, Manuel Lopez; Garcia, Jose A. Tamayo; Gil, Alex Vergara; Lores, Stefan Gutierrez; Acosta, Andry Romero; Villanueva, Gilberto Alonso, E-mail: manolo@cphr.edu.cu, E-mail: jotag@cphr.edu.cu, E-mail: alex@cphr.edu.cu, E-mail: stefan@cphr.edu.cu, E-mail: andy@cphr.edu.cu, E-mail: gilberto@cphr.edu.cu [Centro de Proteccion e Higiene de las Radiaciones (CPHR), La Habana (Cuba)

    2013-07-01

    The acquisition of know how of practical experiences obtained in the repair and maintenance of readers TLD RADOS for five years of work and the request by the International Atomic Energy for signing new Research Contracts (CRP), made possible the CRP 13328, in which the specialist is committed in the course of a year, to deliver educational software in order to train personnel associated with the operation of the TLD readers RADOS. Due to the importance of continuous transfer of knowledge for new generations of technicians and specialists who join our laboratories, the idea came when the first interactive CD that grouped 19 videos, divided into three blocks was ready: learning, repair and maintenance; it was suggested to expand the training for any TLD reader. Thus a much more complete than the first version package emerged. 7 cases were subsequently published in an IAEA TECDOC, 1599 were included.

  5. Techno-Literacy Practices of Emergent Readers

    Directory of Open Access Journals (Sweden)

    Razeale G. Fernandez

    2015-12-01

    Full Text Available – This research explored the techno-literacy practices of emergent readers. The study found that young children experienced a multi-literate practices in their homes that comprised not only print and paper-based literacies but also techno-literacies. That television, games on tablets and phones are the sources of textual pleasure of young children.Families reported that children watched television and play games on tablets/phones regularly. This is due to the limited options of leisure activities available to younger children. Emergent readers are highly focused to televisual text as they demonstrate meaningmaking practices when they constantly ask questions and talk about what they are watching,hence they are active meaning-makers. The learning opportunities which include hand eye coordination, parallel processing, and problem solving skills young children acquired through playing computer games and games on tablet/iPad . Aside from these, they also developed a number of literacy skills as well as how to behave as players.It can be concluded thatexposure to imagery in electronic technologies contributed to the children’s literacy development. The literate identities of emergent readers can be further enhanced as they begin formal schooling. Data indicated that young children are developing techno-literacy practices and this concurs to the findings of Marsh (2010. Therefore, technology serves as a tool for the literacy development of young children. That techno-literacy practices of young children should be valued in school. Future research should also consider attitude, behavior and practices of parents towards the use of technology by children.

  6. Generalized Fibonacci zone plates

    CERN Document Server

    Ke, Jie; Zhu, Jianqiang

    2015-01-01

    We propose a family of zone plates which are produced by the generalized Fibonacci sequences and their axial focusing properties are analyzed in detail. Compared with traditional Fresnel zone plates, the generalized Fibonacci zone plates present two axial foci with equal intensity. Besides, we propose an approach to adjust the axial locations of the two foci by means of different optical path difference, and further give the deterministic ratio of the two focal distances which attributes to their own generalized Fibonacci sequences. The generalized Fibonacci zone plates may allow for new applications in micro and nanophotonics.

  7. Image stacking approach to increase sensitivity of fluorescence detection using a low cost complementary metal-oxide-semiconductor (CMOS) webcam.

    Science.gov (United States)

    Balsam, Joshua; Bruck, Hugh Alan; Kostov, Yordan; Rasooly, Avraham

    2012-01-01

    Optical technologies are important for biological analysis. Current biomedical optical analyses rely on high-cost, high-sensitivity optical detectors such as photomultipliers, avalanched photodiodes or cooled CCD cameras. In contrast, Webcams, mobile phones and other popular consumer electronics use lower-sensitivity, lower-cost optical components such as photodiodes or CMOS sensors. In order for consumer electronics devices, such as webcams, to be useful for biomedical analysis, they must have increased sensitivity. We combined two strategies to increase the sensitivity of CMOS-based fluorescence detector. We captured hundreds of low sensitivity images using a Webcam in video mode, instead of a single image typically used in cooled CCD devices.We then used a computational approach consisting of an image stacking algorithm to remove the noise by combining all of the images into a single image. While video mode is widely used for dynamic scene imaging (e.g. movies or time-lapse photography), it is not used to capture a single static image, which removes noise and increases sensitivity by more than thirty fold. The portable, battery-operated Webcam-based fluorometer system developed here consists of five modules: (1) a low cost CMOS Webcam to monitor light emission, (2) a plate to perform assays, (3) filters and multi-wavelength LED illuminator for fluorophore excitation, (4) a portable computer to acquire and analyze images, and (5) image stacking software for image enhancement. The samples consisted of various concentrations of fluorescein, ranging from 30 μM to 1000 μM, in a 36-well miniature plate. In the single frame mode, the fluorometer's limit-of-detection (LOD) for fluorescein is ∼1000 μM, which is relatively insensitive. However, when used in video mode combined with image stacking enhancement, the LOD is dramatically reduced to 30 μM, sensitivity which is similar to that of state-of-the-art ELISA plate photomultiplier-based readers. Numerous medical

  8. A whole new way of looking at things: the use of Dark Reader technology to detect fluorophors.

    Science.gov (United States)

    Seville, M

    2001-03-01

    The Dark Reader optical system (Clare Chemical Research, Denver, CO, USA) uses relatively low intensity broad-band visible blue light in combination with broad-band optical filters to detect fluorescence with a level of sensitivity that often surpasses that of UV transilluminators and can rival that of laser-based scanners. Applications of DR (Clare Chemical Research) devices include the detection of DNA and SYBR-stained protein samples following, and also during, electrophoresis. Unlike laser-based imaging systems, the fluorescence is directly visible to the user as well as being fully compatible with charge-coupled device (CCD) and Polaroid camera-based detection and imaging. Additionally, the DR optical system functions well in multicolor fluorophor environments. Because the Dark Reader does not emit any UV light, the extent of DNA damage incurred when visualizing DNA samples is drastically reduced compared to the damage produced by a UV device and this can have a significant benefit on downstream cloning protocols. Furthermore, dye photobleaching is minimal, extending the length of time that a fluorescent sample is visible. The inherent flexibility of the DR optical system allows many different configurations of the Dark Reader to be constructed such as transilluminators, hand lamps and integrated transilluminator-electrophoresis units.

  9. Proficient Readers' Reading Behavior in Taiwan: The Study of Young Chinese Readers

    Science.gov (United States)

    Chang, Li-Chun

    2015-01-01

    The purpose of this study was to explore the reading behavior of young proficient Chinese readers at preschool age. Especially, the roles of phonetic skill and Chinese Character recognition in reading comprehension were explored. 10 kindergartens were recruited to participate in the study. Subjects were 72-98 kindergarten children. Instruments…

  10. Metacognition and Reading: Comparing Three Forms of Metacognition in Normally Developing Readers and Readers with Dyslexia.

    Science.gov (United States)

    Furnes, Bjarte; Norman, Elisabeth

    2015-08-01

    Metacognition refers to 'cognition about cognition' and includes metacognitive knowledge, strategies and experiences (Efklides, 2008; Flavell, 1979). Research on reading has shown that better readers demonstrate more metacognitive knowledge than poor readers (Baker & Beall, 2009), and that reading ability improves through strategy instruction (Gersten, Fuchs, Williams, & Baker, 2001). The current study is the first to specifically compare the three forms of metacognition in dyslexic (N = 22) versus normally developing readers (N = 22). Participants read two factual texts, with learning outcome measured by a memory task. Metacognitive knowledge and skills were assessed by self-report. Metacognitive experiences were measured by predictions of performance and judgments of learning. Individuals with dyslexia showed insight into their reading problems, but less general knowledge of how to approach text reading. They more often reported lack of available reading strategies, but groups did not differ in the use of deep and surface strategies. Learning outcome and mean ratings of predictions of performance and judgments of learning were lower in dyslexic readers, but not the accuracy with which metacognitive experiences predicted learning. Overall, the results indicate that dyslexic reading and spelling problems are not generally associated with lower levels of metacognitive knowledge, metacognitive strategies or sensitivity to metacognitive experiences in reading situations.

  11. Error Detection Mechanism for Words and Sentences: A Comparison between Readers with Dyslexia and Skilled Readers

    Science.gov (United States)

    Horowitz-Kraus, Tzipi; Breznitz, Zvia

    2011-01-01

    The activity level of the error monitoring system for processing isolated versus contextual words in Hebrew was studied in adults with dyslexia and skilled readers while committing reading errors. Behavioural measures and event-related potentials were measured during a lexical decision task using words in a list and sentences. Error-related…

  12. Are Avid Readers Lurking in Your Language Arts Classroom? Myths of the Avid Adolescent Reader

    Science.gov (United States)

    Wilson, Nance S.; Kelley, Michelle J.

    2010-01-01

    This article describes a pilot study conducted with 10 identified avid adolescent readers who completed the Adolescent Motivation to Read Profile (AMRP) (Pitcher, Albright, DeLaney, Walker, Seunarienesingh, & Moggie, 2007) that includes both a survey to determine students' self-concept and value of reading and an interview that sheds light on what…

  13. Blue Willow Story Plates

    Science.gov (United States)

    Fontes, Kris

    2009-01-01

    In the December 1997 issue of "SchoolArts" is a lesson titled "Blue Willow Story Plates" by Susan Striker. In this article, the author shares how she used this lesson with her middle-school students many times over the years. Here, she describes a Blue Willow plate painting project that her students made.

  14. Create Your Plate

    Medline Plus

    Full Text Available ... In Memory In Honor Become a Member En Español Type 1 Type 2 About Us Online Community ... Page Text Size: A A A Listen En Español Create Your Plate Create Your Plate is a ...

  15. Create Your Plate

    Medline Plus

    Full Text Available ... Diabetes Meal Plans Create Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook with Heart-Healthy Foods Holiday Meal Planning ... Planning Meals Diabetes Meal Plans and a Healthy Diet Create Your Plate Meal Planning for Vegetarian Diets ...

  16. Monitoring changes in membrane polarity, membrane integrity, and intracellular ion concentrations in Streptococcus pneumoniae using fluorescent dyes.

    Science.gov (United States)

    Clementi, Emily A; Marks, Laura R; Roche-Håkansson, Hazeline; Håkansson, Anders P

    2014-02-17

    Membrane depolarization and ion fluxes are events that have been studied extensively in biological systems due to their ability to profoundly impact cellular functions, including energetics and signal transductions. While both fluorescent and electrophysiological methods, including electrode usage and patch-clamping, have been well developed for measuring these events in eukaryotic cells, methodology for measuring similar events in microorganisms have proven more challenging to develop given their small size in combination with the more complex outer surface of bacteria shielding the membrane. During our studies of death-initiation in Streptococcus pneumoniae (pneumococcus), we wanted to elucidate the role of membrane events, including changes in polarity, integrity, and intracellular ion concentrations. Searching the literature, we found that very few studies exist. Other investigators had monitored radioisotope uptake or equilibrium to measure ion fluxes and membrane potential and a limited number of studies, mostly in Gram-negative organisms, had seen some success using carbocyanine or oxonol fluorescent dyes to measure membrane potential, or loading bacteria with cell-permeant acetoxymethyl (AM) ester versions of ion-sensitive fluorescent indicator dyes. We therefore established and optimized protocols for measuring membrane potential, rupture, and ion-transport in the Gram-positive organism S. pneumoniae. We developed protocols using the bis-oxonol dye DiBAC4(3) and the cell-impermeant dye propidium iodide to measure membrane depolarization and rupture, respectively, as well as methods to optimally load the pneumococci with the AM esters of the ratiometric dyes Fura-2, PBFI, and BCECF to detect changes in intracellular concentrations of Ca(2+), K(+), and H(+), respectively, using a fluorescence-detection plate reader. These protocols are the first of their kind for the pneumococcus and the majority of these dyes have not been used in any other bacterial

  17. Chinese deaf readers have early access to parafoveal semantics.

    Science.gov (United States)

    Yan, Ming; Pan, Jinger; Bélanger, Nathalie N; Shu, Hua

    2015-01-01

    In the present study, we manipulated different types of information available in the parafovea during the reading of Chinese sentences and examined how deaf readers make use of the parafoveal information. Results clearly indicate that although the reading-level matched hearing readers make greater use of orthographic information in the parafovea, parafoveal semantic information is obtained earlier among the deaf readers. In addition, a phonological preview benefit effect was found for the better deaf readers (relative to less-skilled deaf readers), although we also provide an alternative explanation for this effect. Providing evidence that Chinese deaf readers have higher efficiency when processing parafoveal semantics, the study indicates flexibility across individuals in the mechanisms underlying word recognition adapting to the inputs available in the linguistic environment.

  18. Type Testing of Model 7200 Automatic TLD Reader.

    Science.gov (United States)

    Malek Mohammadi, M; Hosseini Pooya, S M

    2017-04-20

    The type testing of measuring devices is one of the most important parts of a quality management system in a personal dosimetry services program. In this study, based upon the International Electrotechnical Commission (IEC) 62387 criteria, a reader-testing program was performed for a home-made personal thermoluminescent dosimetry (TLD) reader. The stability of the reader, the effects of light exposure, temperature and fluctuations of primary power supply on TLD read-outs as the main parameters were investigated in this program. Moreover, this study assesses some important criteria of dosimetry system including the non-linearity of response, reusability, after effect and overload that may include significant contribution in the performance of a reader. The results showed that the TLD reader met all requirements of the IEC for the reader tests by a large margin. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  19. Reception theory and the Christian reader: A preliminary perspective

    Directory of Open Access Journals (Sweden)

    A. L. Comhrink

    1983-03-01

    Full Text Available In recent years there has been a significant shift in emphasis from text- centred criticism in literature to reader-centred criticism. This new field of criticism, called Reception Theory or Reader Response Criticism, denies the immutable nature of the "text” and regards as its object of study the work of literature that is created through the co-constituent creative and interpre­tive acts o f both writer and reader.

  20. Reception theory and the Christian reader: A preliminary perspective

    OpenAIRE

    A. L. Comhrink

    1983-01-01

    In recent years there has been a significant shift in emphasis from text- centred criticism in literature to reader-centred criticism. This new field of criticism, called Reception Theory or Reader Response Criticism, denies the immutable nature of the "text” and regards as its object of study the work of literature that is created through the co-constituent creative and interpre­tive acts o f both writer and reader.

  1. The Reader Response Approach to Teaching of English Literature

    Institute of Scientific and Technical Information of China (English)

    唐芳

    2008-01-01

    During interview with English majors, it was found that a majority of students expressed disappointment with their experience of English literature classes. Students' dislike of learning English literature appears to be the major problem of teaching English literature in China. Reader response approach is a good way to solve this problem. In this paper, I explain the rationale behind the reader response approach, such as its origin, definition, features, assumptions, and strategies. Then I illustrate how reader response approach works as a teaching strategy by presenting several teaching models. At last I evaluate its usefuiness for teachers and present the advantages of reader response approach in Chinese context.

  2. Improved reader for magnetically-encoded ID cards

    Science.gov (United States)

    Wu, T. T.

    1979-01-01

    Hybrid demodulator in electronic card reader for magnetically encoded identification cards, accommodates variations in insertion speeds, yet is simpler and less expensive than equivalent all-digital circuits.

  3. The e-Reader — an Educational or an Entertainment Tool? e-Readers in an Academic Setting

    Directory of Open Access Journals (Sweden)

    Peter Ahlroos

    2012-01-01

    Full Text Available In this paper the authors will discuss a pilot project conducted at the Tritonia Academic Library, Vaasa, in Finland, from September 2010 until May 2011. The project was designed to investigate the application of e-readers in academic settings and to learn how teachers and students experience the use of e-readers in academic education. Four groups of students and one group of teachers used Kindle readers for varied periods of time in different courses. The course material and the textbooks were downloaded on the e-readers. The feedback from the participants was collected through questionnaires and teacher interviews. The results suggest that the e-reader is a future tool for learning, though some features need to be improved before e-readers can really enable efficient learning and researching.

  4. Pixelated neutron image plates

    Science.gov (United States)

    Schlapp, M.; Conrad, H.; von Seggern, H.

    2004-09-01

    Neutron image plates (NIPs) have found widespread application as neutron detectors for single-crystal and powder diffraction, small-angle scattering and tomography. After neutron exposure, the image plate can be read out by scanning with a laser. Commercially available NIPs consist of a powder mixture of BaFBr : Eu2+ and Gd2O3 dispersed in a polymer matrix and supported by a flexible polymer sheet. Since BaFBr : Eu2+ is an excellent x-ray storage phosphor, these NIPs are particularly sensitive to ggr-radiation, which is always present as a background radiation in neutron experiments. In this work we present results on NIPs consisting of KCl : Eu2+ and LiF that were fabricated into ceramic image plates in which the alkali halides act as a self-supporting matrix without the necessity for using a polymeric binder. An advantage of this type of NIP is the significantly reduced ggr-sensitivity. However, the much lower neutron absorption cross section of LiF compared with Gd2O3 demands a thicker image plate for obtaining comparable neutron absorption. The greater thickness of the NIP inevitably leads to a loss in spatial resolution of the image plate. However, this reduction in resolution can be restricted by a novel image plate concept in which a ceramic structure with square cells (referred to as a 'honeycomb') is embedded in the NIP, resulting in a pixelated image plate. In such a NIP the read-out light is confined to the particular illuminated pixel, decoupling the spatial resolution from the optical properties of the image plate material and morphology. In this work, a comparison of experimentally determined and simulated spatial resolutions of pixelated and unstructured image plates for a fixed read-out laser intensity is presented, as well as simulations of the properties of these NIPs at higher laser powers.

  5. Plate removal following orthognathic surgery.

    Science.gov (United States)

    Little, Mhairi; Langford, Richard Julian; Bhanji, Adam; Farr, David

    2015-11-01

    The objectives of this study are to determine the removal rates of orthognathic plates used during orthognathic surgery at James Cook University Hospital and describe the reasons for plate removal. 202 consecutive orthognathic cases were identified between July 2004 and July 2012. Demographics and procedure details were collected for these patients. Patients from this group who returned to theatre for plate removal between July 2004 and November 2012 were identified and their notes were analysed for data including reason for plate removal, age, smoking status, sex and time to plate removal. 3.2% of plates were removed with proportionally more plates removed from the mandible than the maxilla. 10.4% of patients required removal of one or more plate. Most plates were removed within the first post-operative year. The commonest reasons for plate removal were plate exposure and infection. The plate removal rates in our study are comparable to those seen in the literature.

  6. Anisotropic elastic plates

    CERN Document Server

    Hwu, Chyanbin

    2010-01-01

    As structural elements, anisotropic elastic plates find wide applications in modern technology. The plates here are considered to be subjected to not only in plane load but also transverse load. In other words, both plane and plate bending problems as well as the stretching-bending coupling problems are all explained in this book. In addition to the introduction of the theory of anisotropic elasticity, several important subjects have are discussed in this book such as interfaces, cracks, holes, inclusions, contact problems, piezoelectric materials, thermoelastic problems and boundary element a

  7. Plating in Top Agar

    OpenAIRE

    sprotocols

    2014-01-01

    1. Warm plates to room temperature before use. Cold plates causes the top agar to solidify irregularly. DO not warm plates to 37° as the top agar will take forever to solidify. - Prepare top agar as the appropriate liquid medium with 0.7% agar. Keeping 100 mL bottles is convenient. For phages, use λ top agar, which is less rich and yields bigger plaques. - Melt top agar in the microwave completely. Allow the agar to boil after liquification; incompletely melted agar looks liquid, but is...

  8. The structure of subsonic air wakes behind a flat plate

    Energy Technology Data Exchange (ETDEWEB)

    Barreras, F.; Dopazo, C. [Centro Politecnico Superior de Ingenieros Area de Mecanica de Fluidos, Zaragoza (Spain); Lozano, A.; Yates, A.J. [LITEC/CSIC, Maria de Luna 3 E-50015-Zaragoza (Spain)

    1999-04-01

    Acetone vapor planar laser-induced fluorescence has been used to visualize the structure of a subsonic air wake behind a flat plate. Longitudinal and transversal wavelengths have been directly measured from the acquired images. The ratio between them has been calculated to be 2/5. (orig.) With 3 figs., 1 tab., 10 refs.

  9. What do mind readers know and what do we know about mind readers?

    Science.gov (United States)

    Woolley, Jacqueline D

    2014-11-01

    In this commentary, I raise various questions about Kim and Harris's fascinating findings. I ask what kind of knowledge children expect telepathic individuals to have, who children might consider to be good mind readers, why children value telepathy, and how puzzled children are by telepathy. I suggest potential ways to address some of these questions and end by reiterating the importance of probing individual differences in scepticism and credulity.

  10. Create Your Plate

    Medline Plus

    Full Text Available ... blood glucose levels and lose weight. With this method, you fill your plate with more non-starchy ... but changes the portion sizes so you are getting larger portions of non-starchy vegetables and a ...

  11. Create Your Plate

    Medline Plus

    Full Text Available ... blood glucose levels and lose weight. With this method, you fill your plate with more non-starchy ... 4/Box) Taking the guesswork out of portion control has never been easier. It can be a ...

  12. Tectonic Plate Movement.

    Science.gov (United States)

    Landalf, Helen

    1998-01-01

    Presents an activity that employs movement to enable students to understand concepts related to plate tectonics. Argues that movement brings topics to life in a concrete way and helps children retain knowledge. (DDR)

  13. MyPlate

    Science.gov (United States)

    ... our stage of life, situations, preferences, access to food, culture, traditions, and the personal decisions we make over time. All your food and beverage choices count. MyPlate offers ideas and ...

  14. Create Your Plate

    Medline Plus

    Full Text Available ... blood glucose levels and lose weight. With this method, you fill your plate with more non-starchy ... you have an easy portion control solution that works. Last Reviewed: October 8, 2015 Last Edited: September ...

  15. Designing Assemblies Of Plates

    Science.gov (United States)

    Williams, F. W.; Kennedy, D.; Butler, R.; Aston, G.; Anderson, M. S.

    1992-01-01

    VICONOPT calculates vibrations and instabilities of assemblies of prismatic plates. Designed for efficient, accurate analysis of buckling and vibration, and for optimum design of panels of composite materials. Written in FORTRAN 77.

  16. Create Your Plate

    Medline Plus

    Full Text Available ... Your Plate Gluten Free Diets Meal Planning for Vegetarian Diets Cook with Heart-Healthy Foods Holiday Meal Planning What Can I Eat? Making Healthy Food Choices Diabetes Superfoods Non-starchy Vegetables Grains and Starchy Vegetables ...

  17. Create Your Plate

    Medline Plus

    Full Text Available ... Recipes Association Cookbook Recipes Planning Meals Diabetes Meal Plans Create Your Plate Gluten Free Diets Meal Planning ... serving of dairy or both as your meal plan allows. Choose healthy fats in small amounts. For ...

  18. Create Your Plate

    Medline Plus

    Full Text Available ... 1 Diabetes Get Started Safely Get And Stay Fit Types of Activity Weight Loss Assess Your Lifestyle ... manage portion control wherever you are. Now, our best-selling, sectioned to-go plate with easy-sealing ...

  19. Create Your Plate

    Medline Plus

    Full Text Available ... Plate is a simple and effective way to manage your blood glucose levels and lose weight. With ... been easier. It can be a challenge to manage portion control wherever you are. Now, our best- ...

  20. Tectonic Plate Movement.

    Science.gov (United States)

    Landalf, Helen

    1998-01-01

    Presents an activity that employs movement to enable students to understand concepts related to plate tectonics. Argues that movement brings topics to life in a concrete way and helps children retain knowledge. (DDR)

  1. Create Your Plate

    Medline Plus

    Full Text Available ... Carbohydrates Carbohydrate Counting Make Your Carbs Count Glycemic ... to manage portion control wherever you are. Now, our best-selling, sectioned to-go plate with easy-sealing ...

  2. Narcissism, Abjection and the Reader(e of Simone de Beauvoir's Les Belles Images

    Directory of Open Access Journals (Sweden)

    Margaret E. Gray

    2008-01-01

    Full Text Available Discussions of Simone de Beauvoir's last novel, Les Belles Images (1966, tend to be in the mode of apology. The characters are shallow—runs the typical claim—the plot (essentially, Laurence's gradual awakening to her own "belle image" identity of feminine clichés as fulfilled wife, devoted mother, successful professional flimsy and predictable. Yet, in studying a specular dynamic of narcissism and abjection within the novel, we become aware of the discomforting ways in which our own scorn for Laurence and her world is anticipated by the text. As we attend upon the dismantling of Laurence's "belles images," we are made to witness the undoing of our own narcissism. Along the way, we acquire new understanding of an important aspect of this specular relationship: the novel's slippery use of the pronouns "I" and "she" to refer to Laurence, in an unstable grammar that has confounded critics. These pronouns and their imbrication are only too pertinent, I argue, for the reader's relationship to Laurence, and for the ways in which the reader's "I" sets itself loftily apart from Laurence's "she." Yet, in dismantling the reader's assumptions along with those of Laurence, the text offers each a new way forward, beyond the crippling confines of narcissism and abjection.

  3. Origami - Folded Plate Structures

    OpenAIRE

    Buri, Hans Ulrich

    2010-01-01

    This research investigates new methods of designing folded plate structures that can be built with cross-laminated timber panels. Folded plate structures are attractive to both architects and engineers for their structural, spatial, and plastic qualities. Thin surfaces can be stiffened by a series of folds, and thus not only cover space, but also act as load bearing elements. The variation of light and shadow along the folded faces emphasizes the plas...

  4. Fractal multifiber microchannel plates

    Science.gov (United States)

    Cook, Lee M.; Feller, W. B.; Kenter, Almus T.; Chappell, Jon H.

    1992-01-01

    The construction and performance of microchannel plates (MCPs) made using fractal tiling mehtods are reviewed. MCPs with 40 mm active areas having near-perfect channel ordering were produced. These plates demonstrated electrical performance characteristics equivalent to conventionally constructed MCPs. These apparently are the first MCPs which have a sufficiently high degree of order to permit single channel addressability. Potential applications for these devices and the prospects for further development are discussed.

  5. Simplification in Graded Readers: Measuring the Authenticity of Graded Texts

    Science.gov (United States)

    Claridge, Gillian

    2005-01-01

    This study examines the characteristics and quality of simplification in graded readers as compared to those of "normal" authentic English. Two passages from graded readers are compared with the original passages. The comparison uses a computer programme, RANGE (Nation and Heatley, 2003) to analyse the distribution of high and low frequency words…

  6. Eye Movements Reveal Readers' Lexical Quality and Reading Experience

    Science.gov (United States)

    Taylor, Jessica Nelson; Perfetti, Charles A.

    2016-01-01

    Two experiments demonstrate that individual differences among normal adult readers, including lexical quality, are expressed in silent reading at the word level. In the first of two studies we identified major dimensions of variability among college readers and among words using factor analysis. We then examined the effects of these dimensions of…

  7. Chinese Mandarin Advanced Course: Newspaper and Periodicals Reader.

    Science.gov (United States)

    Lipsett, Dale F.; Poelman, James S.

    This reader provides supplementary materials for improvement of reading fluency and expansion of vocabulary for advanced students of Chinese. It familiarizes students with terminology used in mainland China publications. Twenty articles, taken from several newspapers, are compiled in this reader. Vocabulary lists are provided for each selection.…

  8. Miscue Analysis: A Transformative Tool for Researchers, Teachers, and Readers

    Science.gov (United States)

    Goodman, Yetta M.

    2015-01-01

    When a reader produces a response to a written text (the observed response) that is not expected by the listener, the result is called a miscue. Using psychosociolingustic analyses of miscues in the context of an authentic text, miscue analysis provides evidence to discover how readers read. I present miscue analysis history and development and…

  9. Helping the Slow-Reader in the Primary School Classroom.

    Science.gov (United States)

    Burke, Edmund V.

    1988-01-01

    A strategy is proposed for primary teachers to use with children who have reading difficulties. It includes developing a profile of each slow reader, testing each slow reader to determine reading level and specific weaknesses, and using certain practical methods to help solve the problem. (two references) (LB)

  10. Teaching Materials for German. G3: Readers. Teaching Materials Lists.

    Science.gov (United States)

    Centre for Information on Language Teaching, London (England).

    This publication, part of a bibliography of language teaching materials, lists 91 readers. It contains a contents list, annotated entries, an index of authors, editors, compilers and adapters, and a title index. In addition to basic bibliographical details, the following are provided for each entry: an annotation describing the reader, a statement…

  11. Can Bold Typeface Improve Readers' Comprehension and Metacomprehension of Negation?

    Science.gov (United States)

    Margolin, Sara J.

    2013-01-01

    Recent research on negation has demonstrated that while readers are aware that this text construction is difficult, they seem to be able to do little to improve their comprehension. The present research evaluated whether a change in typeface could improve comprehension and metacomprehension of negation. Results indicated that while readers were…

  12. Investigating the Relationship between Connectives and Readers' Reading Comprehension Level

    Science.gov (United States)

    Gençer, Yusuf; ÇetiInkaya, Gökhan

    2015-01-01

    The purpose of this study is to investigate the relationship between connectives in Turkish texts and readers' reading comprehension. Research was conducted with a total of 50 teachers. In the study group, readers' reading comprehension was determined through 10 descriptive texts by using open-ended questions. The results of the analysis revealed…

  13. A Comparative Review of Stratification Texts and Readers

    Science.gov (United States)

    Peoples, Clayton D.

    2012-01-01

    Social stratification is a core substantive area within sociology. There are a number of textbooks and readers available on the market that deal with this central topic. In this article, I conduct a comparative review of (a) four stratification textbooks and (b) four stratification readers. (Contains 2 tables.)

  14. Finding versus Fixing: Self-Monitoring for Readers Who Struggle

    Science.gov (United States)

    Anderson, Nancy L.; Kaye, Elizabeth L.

    2017-01-01

    This article explains how teachers can understand, notice, and supportively respond to readers who struggle with self-monitoring during text reading. The unique strategic processing demands for readers who struggle support the argument that teaching children to find and notice errors is different than fixing a word, or getting it right. Three…

  15. Homophone Effects in Deaf Readers: Evidence from Lexical Decision

    Science.gov (United States)

    Friesen, Deanna C.; Joanisse, Marc F.

    2012-01-01

    The current study examined the nature of deaf readers' phonological processing during online word recognition, and how this compares to similar effects in hearing individuals. Unlike many previous studies on phonological activation, we examined whether deaf readers activated phonological representations for words as opposed to pseudohomophones.…

  16. Chinese Deaf Readers Have Early Access to Parafoveal Semantics

    Science.gov (United States)

    Yan, Ming; Pan, Jinger; Bélanger, Nathalie N.; Shu, Hua

    2015-01-01

    In the present study, we manipulated different types of information available in the parafovea during the reading of Chinese sentences and examined how deaf readers make use of the parafoveal information. Results clearly indicate that although the reading-level matched hearing readers make greater use of orthographic information in the parafovea,…

  17. Investigating the Relationship between Connectives and Readers' Reading Comprehension Level

    Science.gov (United States)

    Gençer, Yusuf; ÇetiInkaya, Gökhan

    2015-01-01

    The purpose of this study is to investigate the relationship between connectives in Turkish texts and readers' reading comprehension. Research was conducted with a total of 50 teachers. In the study group, readers' reading comprehension was determined through 10 descriptive texts by using open-ended questions. The results of the analysis revealed…

  18. Infographics for Educational Purposes: Their Structure, Properties and Reader Approaches

    Science.gov (United States)

    Yildirim, Serkan

    2016-01-01

    Infographics are one of the new educational environments used to provide information to their readers in a visual way. Infographics are designed to provide information to their readers using various visuals such as texts, pictures, drawings, diagrams, graphs, etc. The use of infographics becomes increasingly widespread both in advertising…

  19. Sexism Springs Eternal--in the Reader's Digest.

    Science.gov (United States)

    Zimbardo, Philip G.; Meadow, Wendy

    This document reports on an empirical investigation of anti-women humor appearing in the Reader's Digest over three decades, revealing the operation of an unconscious sexist ideology. A systematic analysis was made of 1,069 jokes appearing in two featured columns of the Reader's Digest for the two-year periods 1947-48, 1957-58, and 1967-68.…

  20. Learning to Choose: The Hidden Art of the Enthusiastic Reader

    Science.gov (United States)

    Mackey, Margaret

    2014-01-01

    Often the difference between a successful and unsuccessful reader is that the former is simply better at finding enjoyable books to read. The capacity to select appealing reading is not developed in classrooms where the decision about what book to read is made by someone other than the reader: the teacher, the curriculum planner, the person who…

  1. Breaking the Silence: Toward Improving LGBTQ Representation in Composition Readers

    Science.gov (United States)

    Hudson, John

    2014-01-01

    Lesbian-Gay-Bisexual-Transgender-Queer (LGBTQ) representation in composition readers remains limited and is frequently nonexistent. In addition, the LGBTQ-related materials that do find their way into composition readers are often problematic. In this essay I explain why WPAs and composition teachers should be concerned about LGBTQ representation…

  2. Comparing Reading Processing Strategies of Second Language Readers

    Directory of Open Access Journals (Sweden)

    Parilah M. Shah

    2010-01-01

    Full Text Available Problem statement: The message that a writer tries to convey in a text would be subjected to several interpretations by readers. Apparently, reading is a complex process of getting input. A well-known researcher offers two views of reading: (i reading is a process of decoding written symbols and (ii reading is a process of reconstructing meaning. It has also been proposed that readers used reading processing strategies in the process of understanding text. Most language educators are not aware of the specific reading strategies that second language readers utilize. Therefore, it is deemed necessary to conduct a study that could explore the specific types of strategies used and to compare the strategies utilized by readers of differing abilities. Approach: A study is conducted to examine the second language readers use of reading strategies at the Malaysian secondary schools. They read a piece of reading material, and then respond to questionnaires concerning reading strategies such as supervising strategies, support strategies and paraphrase strategies. Results: The findings indicate that there are differences in reading strategies used by second language readers of differing abilities for some of the question items. The results suggest the need to address the incorporation of reading strategy instruction in the language curriculum in order to produce more efficient readers. Conclusion: This investigation is another useful contribution to the applied linguistics research since second language educators would gain better insight into the readers comprehension process.

  3. Accelerated Reader/Reading Renaissance. What Works Clearinghouse Intervention Report

    Science.gov (United States)

    What Works Clearinghouse, 2007

    2007-01-01

    The Accelerated Reader/Reading Renaissance program (now called Accelerated Reader Best Classroom Practices) is a guided reading intervention in which teachers direct student reading of text. It involves two components. Reading Renaissance, the first component, is a set of recommended principles on guided reading (or teachers' direction of…

  4. Designating Reader Perspective to Increase Comprehension and Interest

    Science.gov (United States)

    Ramsay, Crystal M.; Sperling, Rayne A.

    2010-01-01

    In three experiments we examined whether reader perspective on a long expository text could be manipulated such that increased text interest and enhancement of two comprehension outcomes would result. In Experiment 1 we verified the viability of a new text for experimental purposes. We then assigned readers a perspective before reading in…

  5. Stance, Navigation, and Reader Response in Expository Hypertext

    Science.gov (United States)

    McEneaney, John E.; Li, Ledong; Allen, Kris; Guzniczak, Lizabeth

    2009-01-01

    This article reports on two studies investigating reader stance, navigation, and response in expository hypertext. Subjects in the studies included 69 and 147 adult readers prompted to adopt either an efferent or aesthetic stance when reading a 36-node expository hypertext. Reading was followed by recall and essay writing tasks. Results of the…

  6. How Think-and-Feel-Aloud Instruction Influences Poetry Readers

    Science.gov (United States)

    Eva-Wood, Amy L.

    2004-01-01

    Assuming readers' emotional responses can inform literary analysis, this study of poetry readers featured an instructional intervention that involved modeling both cognitive and affective reading processes through a think-and-feel-aloud pedagogy. Eleventh-grade students in 2 conditions participated in a 4-week unit on reading poetry. Control group…

  7. Vibration and Buckling of Web Plate of the Plate Girder

    OpenAIRE

    高橋, 和雄; 呉, 明強; 中澤, 聡志; 筑紫, 宏之

    1998-01-01

    The vibration and buckling of the web of the plate girder are studied in this paper. The small deflection theory of the thin plate is used. The finite strip method is employed to solve vibration and buckling of the plate girder. Natural frequenies of buckling properties are shown for various plate girder bridges.

  8. FLEX: fluorescence explorer

    Science.gov (United States)

    Stoll, Marc-Ph.; Court, Andrew; Smorenburg, Kees; Visser, Huib; Crocco, Luiggi; Heilimo, Jyro; Honig, Andre

    1999-12-01

    FLEX is a scientifically driven space mission to provide demonstration/validation of the instrumentation and technique for measuring the natural fluorescence of vegetation in the Fraunhofer lines. The payload consists of high spectral resolution (0.1 - 0.3 nm) CCD imaging grating spectrometer with two channels: one in the red (648 - 664 nm) and one in the blue (391 - 438 nm) for working with several Fraunhofer lines. The across track FOV is 8.4 degrees; ground spatial resolution is better than 0.5 X 0.5 km2. To increase the S/N ratio a steering mirror will be used, if necessary, to 'freeze' the image and also to provide plus or minus 4 degrees across track depointing. Calibration is made by viewing the sun via a diffuser plate switched into the telescope field of view. A separate CCD camera will allow cloud detection and scene identification. A TIR radiometer will provide simultaneous surface temperature measurements. The spacecraft, overall mass estimated at 200 kg, is derived from the ASI-MITA bus which provides all the necessary subsystems and stabilized platform. By use of on-board storage, ground requirements for satellite control and data link are minimized; the possibility of local stations for real time reception/distribution is also envisaged. Provisional orbit characteristics are: LEO sun synchronous, 500 - 900 km altitude. Priority will be given to highest revisit frequency on a sufficient number of selected test sites.

  9. In situ study of the impact of inter- and intra-reader variability on region of interest (ROI) analysis in preclinical molecular imaging.

    Science.gov (United States)

    Habte, Frezghi; Budhiraja, Shradha; Keren, Shay; Doyle, Timothy C; Levin, Craig S; Paik, David S

    2013-01-01

    We estimated reader-dependent variability of region of interest (ROI) analysis and evaluated its impact on preclinical quantitative molecular imaging. To estimate reader variability, we used five independent image datasets acquired each using microPET and multispectral fluorescence imaging (MSFI). We also selected ten experienced researchers who utilize molecular imaging in the same environment that they typically perform their own studies. Nine investigators blinded to the data type completed the ROI analysis by drawing ROIs manually that delineate the tumor regions to the best of their knowledge and repeated the measurements three times, non-consecutively. Extracted mean intensities of voxels within each ROI are used to compute the coefficient of variation (CV) and characterize the inter- and intra-reader variability. The impact of variability was assessed through random samples iterated from normal distributions for control and experimental groups on hypothesis testing and computing statistical power by varying subject size, measured difference between groups and CV. The results indicate that inter-reader variability was 22.5% for microPET and 72.2% for MSFI. Additionally, mean intra-reader variability was 10.1% for microPET and 26.4% for MSFI. Repeated statistical testing showed that a total variability of CV readers, which may adversely affect statistical power and erroneously lead to negative study outcomes.

  10. Readers' Responses When Characters Act on Completed Goals: Impact of Characters' Mental States and Readers' Task Focus

    Science.gov (United States)

    Foy, Jeffrey E.; Gerrig, Richard J.

    2014-01-01

    Research has demonstrated that readers track the objective status of characters' goals (i.e., whether the goals have been completed). We suggest that readers also use characters' subjective representations--characters' mental states with respect to goals--to comprehend actions. We explored circumstances in which local information about characters'…

  11. Handheld E-Book Readers and Scholarship Report and Reader Survey: ACLS Humanities E-Book. White Paper No. 3

    Science.gov (United States)

    Gielen, Nina

    2010-01-01

    This report describes a conversion experiment and subsequent reader survey conducted by the American Council of Learned Societies (ACLS) Humanities E-Book (HEB) in late 2009 and early 2010 to assess the viability of using scholarly monographs with handheld e-readers. As sample content, HEB selected six titles from its own online collection, three…

  12. Readers' Responses When Characters Act on Completed Goals: Impact of Characters' Mental States and Readers' Task Focus

    Science.gov (United States)

    Foy, Jeffrey E.; Gerrig, Richard J.

    2014-01-01

    Research has demonstrated that readers track the objective status of characters' goals (i.e., whether the goals have been completed). We suggest that readers also use characters' subjective representations--characters' mental states with respect to goals--to comprehend actions. We explored circumstances in which local information about characters'…

  13. Co-editors’ Note to Readers

    Directory of Open Access Journals (Sweden)

    Wen-hsin Yeh

    2015-06-01

    Full Text Available Dear Cross-Currents readers, We are pleased to present you with the fifteenth quarterly issue of the Cross-Currents e-journal. The research articles in the June 2015 issue—guest edited by Elena Barabantseva (University of Manchester, Xiang Biao (University of Oxford, and Antonia Chao (Tunghai University—explore the theme “Governing Marriage Migrations: Perspectives from Mainland China and Taiwan.” The issue includes five articles by scholars from Japan, Germany, the UK, Taiwan, and Hong Kong who are engaged in critical analysis of cross-border migration for the purpose of marriage in the People’s Republic of China and Taiwan as a subject of governance. As the guest editors emphasize in their introduction, cross-border marriage is “perceived to be inseparable from a wide range of other issues, such as sexual morality, family norms, national identity, and border security.” The contributors—Hongfang Hao (Kyoto University, Caroline Grillot (Max Planck Institute for Social Anthropology, Elena Barabantseva (University of Manchester, Mei-Hua Chen (National Sun Yat-sen University, and Hsun-Hui Tseng (Chinese University of Hong Kong—offer valuable new insights on international marriage migration in their multidisciplinary and fieldwork-based studies...

  14. Bending and stretching of plates

    CERN Document Server

    Mansfield, E H; Hemp, W S

    1964-01-01

    The Bending and Stretching of Plates deals with elastic plate theory, particularly on small- and large-deflexion theory. Small-deflexion theory concerns derivation of basic equations, rectangular plates, plates of various shapes, plates whose boundaries are amenable to conformal transformation, plates with variable rigidity, and approximate methods. Large-deflexion theory includes general equations and some exact solutions, approximate methods in large-deflexion theory, asymptotic large-deflexion theories for very thin plates. Asymptotic theories covers membrane theory, tension field theory, a

  15. Examining College Students’ Reading Behaviors and Needs for Ebook Readers

    Directory of Open Access Journals (Sweden)

    Wei-Jane Lin

    2012-12-01

    Full Text Available This study investigates college students’ reading behaviors and attitudes toward print and electronic carriers of books. With the prosperity and variety of ebook readers currently, it is important to understand the genuine needs of the users to better leverage the features and functions of the ebook readers. The methodology of survey research is conducted to explore 76 undergraduate students’ experiences, preferences and appraisals toward reading via the print books and ebook readers. Generally the college students possess positive attitudes toward and are willing to take advantage of the ebook and ebook reader services provided by libraries, but the current rate of use is low. The results suggested that student adoption of ebook readers are affected by their goals of reading. The college students prefer ebook readers significantly for leisure reading, and highly demand the functionality of multimedia presentations and file management. When reading academic contexts, the college students preferred hardcovers and online ebooks via the Web browser, and they value the functions of searching, marking and file management on ebook readers especially. [Article content in Chinese

  16. Anterior cervical plating

    Directory of Open Access Journals (Sweden)

    Gonugunta V

    2005-01-01

    Full Text Available Although anterior cervical instrumentation was initially used in cervical trauma, because of obvious benefits, indications for its use have been expanded over time to degenerative cases as well as tumor and infection of the cervical spine. Along with a threefold increase in incidence of cervical fusion surgery, implant designs have evolved over the last three decades. Observation of graft subsidence and phenomenon of stress shielding led to the development of the new generation dynamic anterior cervical plating systems. Anterior cervical plating does not conclusively improve clinical outcome of the patients, but certainly enhances the efficacy of autograft and allograft fusion and lessens the rate of pseudoarthrosis and kyphosis after multilevel discectomy and fusions. A review of biomechanics, surgical technique, indications, complications and results of various anterior cervical plating systems is presented here to enable clinicians to select the appropriate construct design.

  17. License plate detection algorithm

    Science.gov (United States)

    Broitman, Michael; Klopovsky, Yuri; Silinskis, Normunds

    2013-12-01

    A novel algorithm for vehicle license plates localization is proposed. The algorithm is based on pixel intensity transition gradient analysis. Near to 2500 natural-scene gray-level vehicle images of different backgrounds and ambient illumination was tested. The best set of algorithm's parameters produces detection rate up to 0.94. Taking into account abnormal camera location during our tests and therefore geometrical distortion and troubles from trees this result could be considered as passable. Correlation between source data, such as license Plate dimensions and texture, cameras location and others, and parameters of algorithm were also defined.

  18. RFID reader design for identification of industrial metallic objects

    Science.gov (United States)

    Szczurkowski, M.; Jankowski, H.; Worek, C.; Maksymowicz, L. J.

    2006-10-01

    An electronic circuit design of the RFID (Radio Frequency Identification) reader was the aim of our research. This device is designed for identification of transponders, which are present on the surface of metal elements or inside machine elements made of metal. In order to achieve that, we optimized the antenna reader circuit, that works in near field (magnetic coupling), by means of field flux lines analysis (using finite elements method) and experimental data collecting. Finally, one obtaines a specific shaped magnetic field around the reader.

  19. Teenagers Poor Readers: Evaluation of Basic Cognitive Process

    Directory of Open Access Journals (Sweden)

    Rosa del Carmen Flores Macías

    2015-04-01

    Full Text Available The present study aims to investigate the cognitive processes associated with reading difficulties of teenage poor readers. Several studies suggest that this population presents a poor comprehension, despite reading the words properly and have good phonological skills (which distinguishes them from a population with dyslexia. With a comparative cross-sectional design the Sicole-R multimedia battery, which assesses basic cognitive processes related to reading, was applied to participants. Results indicate that poor reader students exhibit a lower performance than normal readers in phonological awareness, orthographic processing and processing syntax, although only the latter comparison was statistically significant.

  20. Usability and Accessibility of eBay by Screen Reader

    Science.gov (United States)

    Buzzi, Maria Claudia; Buzzi, Marina; Leporini, Barbara; Akhter, Fahim

    The evolution of Information and Communication Technology and the rapid growth of the Internet have fuelled a great diffusion of eCommerce websites. Usually these sites have complex layouts crowded with active elements, and thus are difficult to navigate via screen reader. Interactive environments should be properly designed and delivered to everyone, including the blind, who usually use screen readers to interact with their computers. In this paper we investigate the interaction of blind users with eBay, a popular eCommerce website, and discuss how using the W3C Accessible Rich Internet Applications (WAI-ARIA) suite could improve the user experience when navigating via screen reader.

  1. E-Books on the Mobile E-Reader

    Directory of Open Access Journals (Sweden)

    Chulyun Kim

    2013-01-01

    Full Text Available The market for e-books has been established during the past several years. Many e-book readers are commercially available, and millions of e-book titles are available for purchase or free download. E-paper technology has matured enough to be used as the screen for dedicated e-book readers which make reading possible with the familiar feel of reading printed books. In this paper, we provide a comprehensive review of the status of the e-book, including the architecture and features of e-book readers, market adoption and e-book industry ecosystem.

  2. Casimir force between metal plate and dielectric plate

    Institute of Scientific and Technical Information of China (English)

    刘中柱; 邵成刚; 罗俊

    1999-01-01

    The Casimir effect between metal plate and dielectric plate is discussed with 1+1-dimensional potential model without using cut-off method. Calculation shows that the Casimir force between metal plate and dielectric plate is determined not only by the potential V0, the dielectric thickness and the distance α between the metal plate and dielectric plate, but also by the dimension of the vessel. When α is far less than the dimension of the vessel, the Casimir force Fc∝α(-1); conversely Fc∝α-2. This result is significant for Casimir force experiment.

  3. Fundamentals of fluorescence and fluorescence microscopy.

    Science.gov (United States)

    Wolf, David E

    2013-01-01

    This chapter discusses the fundamental physics of fluorescence. The application of fluorescence to microscopy represents an important transition in the development of microscopy, particularly as it applies to biology. It enables quantitating the amounts of specific molecules within a cell, determining whether molecules are complexing on a molecular level, measuring changes in ionic concentrations within cells and organelles, and measuring molecular dynamics. This chapter also discusses the issues important to quantitative measurement of fluorescence and focuses on four of quantitative measurements of fluorescence--boxcar-gated detection, streak cameras, photon correlation, and phase modulation. Although quantitative measurement presents many pitfalls to the beginner, it also presents significant opportunities to one skilled in the art. This chapter also examines how fluorescence is measured in the steady state and time domain and how fluorescence is applied in the modern epifluorescence microscope.

  4. Fundamentals of fluorescence microscopy exploring life with light

    CERN Document Server

    Mondal, Partha Pratim

    2014-01-01

    This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence  factors and phenomena such as, molecular cro...

  5. Mixed Sequence Reader: A Program for Analyzing DNA Sequences with Heterozygous Base Calling

    Science.gov (United States)

    Chang, Chun-Tien; Tsai, Chi-Neu; Tang, Chuan Yi; Chen, Chun-Houh; Lian, Jang-Hau; Hu, Chi-Yu; Tsai, Chia-Lung; Chao, Angel; Lai, Chyong-Huey; Wang, Tzu-Hao; Lee, Yun-Shien

    2012-01-01

    The direct sequencing of PCR products generates heterozygous base-calling fluorescence chromatograms that are useful for identifying single-nucleotide polymorphisms (SNPs), insertion-deletions (indels), short tandem repeats (STRs), and paralogous genes. Indels and STRs can be easily detected using the currently available Indelligent or ShiftDetector programs, which do not search reference sequences. However, the detection of other genomic variants remains a challenge due to the lack of appropriate tools for heterozygous base-calling fluorescence chromatogram data analysis. In this study, we developed a free web-based program, Mixed Sequence Reader (MSR), which can directly analyze heterozygous base-calling fluorescence chromatogram data in .abi file format using comparisons with reference sequences. The heterozygous sequences are identified as two distinct sequences and aligned with reference sequences. Our results showed that MSR may be used to (i) physically locate indel and STR sequences and determine STR copy number by searching NCBI reference sequences; (ii) predict combinations of microsatellite patterns using the Federal Bureau of Investigation Combined DNA Index System (CODIS); (iii) determine human papilloma virus (HPV) genotypes by searching current viral databases in cases of double infections; (iv) estimate the copy number of paralogous genes, such as β-defensin 4 (DEFB4) and its paralog HSPDP3. PMID:22778697

  6. Mixed Sequence Reader: A Program for Analyzing DNA Sequences with Heterozygous Base Calling

    Directory of Open Access Journals (Sweden)

    Chun-Tien Chang

    2012-01-01

    Full Text Available The direct sequencing of PCR products generates heterozygous base-calling fluorescence chromatograms that are useful for identifying single-nucleotide polymorphisms (SNPs, insertion-deletions (indels, short tandem repeats (STRs, and paralogous genes. Indels and STRs can be easily detected using the currently available Indelligent or ShiftDetector programs, which do not search reference sequences. However, the detection of other genomic variants remains a challenge due to the lack of appropriate tools for heterozygous base-calling fluorescence chromatogram data analysis. In this study, we developed a free web-based program, Mixed Sequence Reader (MSR, which can directly analyze heterozygous base-calling fluorescence chromatogram data in .abi file format using comparisons with reference sequences. The heterozygous sequences are identified as two distinct sequences and aligned with reference sequences. Our results showed that MSR may be used to (i physically locate indel and STR sequences and determine STR copy number by searching NCBI reference sequences; (ii predict combinations of microsatellite patterns using the Federal Bureau of Investigation Combined DNA Index System (CODIS; (iii determine human papilloma virus (HPV genotypes by searching current viral databases in cases of double infections; (iv estimate the copy number of paralogous genes, such as β-defensin 4 (DEFB4 and its paralog HSPDP3.

  7. Mixed sequence reader: a program for analyzing DNA sequences with heterozygous base calling.

    Science.gov (United States)

    Chang, Chun-Tien; Tsai, Chi-Neu; Tang, Chuan Yi; Chen, Chun-Houh; Lian, Jang-Hau; Hu, Chi-Yu; Tsai, Chia-Lung; Chao, Angel; Lai, Chyong-Huey; Wang, Tzu-Hao; Lee, Yun-Shien

    2012-01-01

    The direct sequencing of PCR products generates heterozygous base-calling fluorescence chromatograms that are useful for identifying single-nucleotide polymorphisms (SNPs), insertion-deletions (indels), short tandem repeats (STRs), and paralogous genes. Indels and STRs can be easily detected using the currently available Indelligent or ShiftDetector programs, which do not search reference sequences. However, the detection of other genomic variants remains a challenge due to the lack of appropriate tools for heterozygous base-calling fluorescence chromatogram data analysis. In this study, we developed a free web-based program, Mixed Sequence Reader (MSR), which can directly analyze heterozygous base-calling fluorescence chromatogram data in .abi file format using comparisons with reference sequences. The heterozygous sequences are identified as two distinct sequences and aligned with reference sequences. Our results showed that MSR may be used to (i) physically locate indel and STR sequences and determine STR copy number by searching NCBI reference sequences; (ii) predict combinations of microsatellite patterns using the Federal Bureau of Investigation Combined DNA Index System (CODIS); (iii) determine human papilloma virus (HPV) genotypes by searching current viral databases in cases of double infections; (iv) estimate the copy number of paralogous genes, such as β-defensin 4 (DEFB4) and its paralog HSPDP3.

  8. Nuclear reactor alignment plate configuration

    Energy Technology Data Exchange (ETDEWEB)

    Altman, David A; Forsyth, David R; Smith, Richard E; Singleton, Norman R

    2014-01-28

    An alignment plate that is attached to a core barrel of a pressurized water reactor and fits within slots within a top plate of a lower core shroud and upper core plate to maintain lateral alignment of the reactor internals. The alignment plate is connected to the core barrel through two vertically-spaced dowel pins that extend from the outside surface of the core barrel through a reinforcement pad and into corresponding holes in the alignment plate. Additionally, threaded fasteners are inserted around the perimeter of the reinforcement pad and into the alignment plate to further secure the alignment plate to the core barrel. A fillet weld also is deposited around the perimeter of the reinforcement pad. To accomodate thermal growth between the alignment plate and the core barrel, a gap is left above, below and at both sides of one of the dowel pins in the alignment plate holes through with the dowel pins pass.

  9. Create Your Plate

    Medline Plus

    Full Text Available ... 1 Type 2 About Us Online Community Meal Planning Sign In Search: Search More Sites Search ≡ Are ... Fitness Home Food MyFoodAdvisor Recipes Association Cookbook Recipes Planning Meals Diabetes Meal Plans Create Your Plate Gluten ...

  10. Create Your Plate

    Medline Plus

    Full Text Available ... Create Your Plate is a simple and effective way to manage your blood glucose levels and lose weight. With ... year of delicious meals to help prevent and manage diabetes. Healthy Recipes: ... to your day with this guide. Ways to Give: Wear Your Cause on Your Sleeve - ...

  11. The Plate Tectonics Project

    Science.gov (United States)

    Hein, Annamae J.

    2011-01-01

    The Plate Tectonics Project is a multiday, inquiry-based unit that facilitates students as self-motivated learners. Reliable Web sites are offered to assist with lessons, and a summative rubric is used to facilitate the holistic nature of the project. After each topic (parts of the Earth, continental drift, etc.) is covered, the students will…

  12. Create Your Plate

    Medline Plus

    Full Text Available ... tax-deductible gift today can fund critical diabetes research and support vital diabetes education services that improve the ... way to manage your blood glucose levels and lose weight. With this method, you fill your plate with more non-starchy ...

  13. Create Your Plate

    Medline Plus

    Full Text Available ... 1 Type 2 About Us Online Community Meal Planning Sign In Search: Search More Sites Search ≡ Are ... Fitness Home Food MyFoodAdvisor Recipes Association Cookbook Recipes Planning Meals Diabetes Meal Plans Create Your Plate Gluten ...

  14. Create Your Plate

    Medline Plus

    Full Text Available ... tax-deductible gift today can fund critical diabetes research and support vital diabetes education services that improve the ... way to manage your blood glucose levels and lose weight. With this method, you fill your plate with more non-starchy ...

  15. Create Your Plate

    Medline Plus

    Full Text Available ... unsweetened tea or coffee. Featured Product Precise Portions® Go Healthy Travel Pack (4/Box) Taking the guesswork ... you are. Now, our best-selling, sectioned to-go plate with easy-sealing lid is offered in ...

  16. Create Your Plate

    Medline Plus

    Full Text Available ... manage portion control wherever you are. Now, our best-selling, sectioned to-go plate with easy-sealing lid is offered in a 4-pack. Whether ... Research & Practice We Are Research Leaders We Support Your Doctor ...

  17. INL HIP Plate Fabrication

    Energy Technology Data Exchange (ETDEWEB)

    B. H. Park; C. R. Clark; J. F. Jue

    2010-02-01

    This document outlines the process used to bond monolithic fuel plates by Hot Isostatic Pressing (HIP). This method was developed at Idaho National Laboratory (INL) for the Reduced Enrichment for Research and Test Reactors (RERTR) program. These foils have been used in a number of irradiation experiments in support of the United States Global Threat Reduction Initiative (GTRI) program.

  18. The Plate Tectonics Project

    Science.gov (United States)

    Hein, Annamae J.

    2011-01-01

    The Plate Tectonics Project is a multiday, inquiry-based unit that facilitates students as self-motivated learners. Reliable Web sites are offered to assist with lessons, and a summative rubric is used to facilitate the holistic nature of the project. After each topic (parts of the Earth, continental drift, etc.) is covered, the students will…

  19. Create Your Plate

    Medline Plus

    Full Text Available ... Planning Meals > Create Your Plate Share: Print Page Text Size: A A A Listen En Español Create ... somewhere in between, you have an easy portion control solution that works. Last Reviewed: October 8, 2015 Last Edited: ... Cost of Diabetes Advocate Toolkit Call to Congress Research & ...

  20. A Smartphone-Based Colorimetric Reader for Human C-Reactive Protein Immunoassay.

    Science.gov (United States)

    Venkatesh, A G; van Oordt, Thomas; Schneider, E Marion; Zengerle, Roland; von Stetten, Felix; Luong, John H T; Vashist, Sandeep Kumar

    2017-01-01

    A smartphone-based colorimetric reader (SBCR), comprising a Samsung Galaxy SIII mini, a gadget (iPAD mini, iPAD4, or iPhone 5s) and a custom-made dark hood and base holder assembly, is used for human C-reactive protein (CRP) immunoassay. A 96-well microtiter plate (MTP) is positioned on the gadget's screensaver to provide white light-based bottom illumination only in the specific regions corresponding to the well's bottom. The images captured by the smartphone's back camera are analyzed by a novel image processing algorithm. Based on one-step kinetics-based human C-reactive protein immunoassay (IA), SBCR is evaluated and compared with a commercial MTP reader (MTPR). For analysis of CRP spiked in diluted human whole blood and plasma as well as CRP in clinical plasma samples, SBCR exhibits the same precision, dynamic range, detection limit, and sensitivity as MTPR for the developed IA (DIA). Considering its compactness, low cost, advanced features and a remarkable computing power, SBCR is an ideal point-of-care (POC) colorimetric detection device for the next-generation of cost-effective POC testing (POCT).

  1. ERIC/RCS: Reader Response in the Classroom.

    Science.gov (United States)

    Holbrook, Hilary Taylor

    1987-01-01

    Explores briefly the New Criticism that dominated literature instruction until recently and then provides an overview of reader response theory and how response approaches can be used in the classroom to enhance reading. (NKA)

  2. What Is That Lapping the Miles?: Responding to Reader Response.

    Science.gov (United States)

    Seib, Kenneth

    1995-01-01

    Responds to an article in an earlier issue of this journal about using reading response in a college literature classroom. Argues that the use of reader-response theory with two-year college students requires some caution. (SR)

  3. The Reading-Writing Connection for Struggling Readers.

    Science.gov (United States)

    Aihara, Kathleen; Au, Kathryn; Carroll, Jacquelin; Nakanishi, Patricia; Scheu, Judith; Wong-Kam, Jo Ann

    1999-01-01

    Describes and discusses six recent books for teachers that illuminate, from different perspectives, aspects of the reading-writing connection for struggling readers, and how to teach reading and writing effectively. (SR)

  4. "Pro Patria": Young Readers and the "Great War."

    Science.gov (United States)

    Fox, Geoff

    1985-01-01

    Provides a thematic presentation of poems written for young readers during the First World War. Draws heavily upon the poems and articles that appeared in "The Boy's Own Paper," a British popular magazine of the period. (HOD)

  5. Are e-readers suitable tools for scholarly work?

    CERN Document Server

    Schomisch, Siegfried; Mayr, Philipp

    2012-01-01

    This paper aims to offer insights into the usability, acceptance and limitations of e-readers with regard to the specific requirements of scholarly text work. To fit into the academic workflow non-linear reading, bookmarking, commenting, extracting text or the integration of non-textual elements must be supported. A group of social science students were questioned about their experiences with electronic publications for study purposes. This same group executed several text-related tasks with the digitized material presented to them in two different file formats on four different e-readers. Their performances were subsequently evaluated by means of frequency analyses in detail. Findings - e-Publications have made advances in the academic world; however e-readers do not yet fit seamlessly into the established chain of scholarly text-processing focusing on how readers use material during and after reading. Our tests revealed major deficiencies in these techniques. With a small number of participants (n=26) quali...

  6. 'Reading' bubble chamber pictures with the Spiral Reader eyes.

    CERN Multimedia

    1974-01-01

    Interactive techniques were widely used to study bubble chamber pictures. After a visual scanning of the pictures and a vertex measurement on a Shivamatic, the Spiral Reader allowed the track polar coordinates to be easily measured. See photo 7408137X

  7. Students Explore Text, Themselves, and Life through Reader Response.

    Science.gov (United States)

    Moutray, Carol L.; Pollard, Jean Ann; McGinley, Jill

    2001-01-01

    Examines the benefits of reader response by means of student letters to authors. Notes that students involved in response writing become more interested in reading, are more reflective individuals, and more responsible for monitoring and resolving their own questions. (SD)

  8. 3 Editorial message Dear readers, The Rwandan Journal of ...

    African Journals Online (AJOL)

    Amy Stambach

    Rwandan Journal of Education - Volume 4 No 1 (2017). 3. Editorial message. Dear readers,. The Rwandan Journal of Education strives to maintain its standard as a scholarly, peer-reviewed ... governance, management and others.

  9. A halochromic stimuli-responsive reversible fluorescence switching 3, 4, 9, 10-perylene tetracarboxylic acid dye for fabricating rewritable platform

    Science.gov (United States)

    Hariharan, P. S.; Pitchaimani, J.; Madhu, Vedichi; Anthony, Savarimuthu Philip

    2017-02-01

    3, 4, 9, 10-perylene tetracarboxylic acid (PTCA), a strongly fluorescent water soluble dye with halochromic functionality showed pH dependent reversible fluorescence switching. The strong fluorescence of PTCA (Φf = 0.67) in basic medium was completely quenched upon acidification. The fluorescent PTCA has been transferred on to a solid substrate (filter paper and glass plate) that also showed reversible off-on fluorescence switching by acid/base and drying/water vapor exposure. The reversible fluorescence switching of PTCA could be of potential interest for fabricating rewritable fluorescent medium.

  10. Tailored nanoporous gold for ultrahigh fluorescence enhancement.

    Science.gov (United States)

    Lang, X Y; Guan, P F; Fujita, T; Chen, M W

    2011-03-07

    We report molecular fluorescence enhancement of free-standing nanoporous gold in which the nanoporosity can be arbitrarily tailored by the combination of dealloying and electroless gold plating. The nanoporous gold fabricated by this facile method possesses unique porous structures with large gold ligaments and very small pores, and exhibits significant improvements in surface enhanced fluorescence as well as structure rigidity. It demonstrates that the confluence effect of improved quantum yield and excitation of fluorophores is responsible for the large fluorescence enhancement due to the near-field enhancement of nanoporous gold, which arises from the strong electromagnetic coupling between neighboring ligaments and the weakening of plasmon damping of the large ligaments because of the small pore size and large ligament size, respectively.

  11. Making a Commitment to Strategic-Reader Training

    Institute of Scientific and Technical Information of China (English)

    FREDRICKA L. STOLLER; REIKO KOMIYAMA

    2013-01-01

    Skilled readers by definition are strategic; they are able to use a repertoire of reading strategies , flexibly and in meaningful combinations , to achieve their reading comprehension goals . Thus , one of the aims of foreign and second language (L2) reading curricula should be to move students toward becoming more strategic readers . This curricular orientation can be best achieved when a strong commitment is made to strategic-reader training as a regular and consistent component of instruction across the curriculum . To explore this stance , we examine the reading strategies used by skilled readers , contrast teaching strategies with training strategic readers ( i .e . , strategic-reader training) , and examine five strategic-reader training approaches from first language contexts that can be adapted by L2 professionals to enhance the reading instruction offered in their L 2 classes . The five approaches targeted for exploration include Directed Reading-Thinking Activity , Reciprocal Teaching , Transactional Strategies Instruction , Questioning the Author , and Concept-Oriented Reading Instruction . Though distinct from one another , they all acknowledge the importance of explicit explanations about strategies (or reminders about the use of select strategies) , teacher modeling , scaffolded tasks , active student engagement , student practice , classroom discussions of strategy use , and the gradual release of responsibility to students who eventually decide for themselves (and/or with peers) when , where , and why to use which strategies to achieve their comprehension goals . We conclude with a discussion of the challenges , and suggestions for overcoming them , that L 2 teachers and students often face in making a commitment to strategic-reader training .

  12. Optimised inductively coupled reader antennas for smart HF RFID systems.

    OpenAIRE

    Soodmand, Soheyl

    2015-01-01

    The Internet of things (IoT) refers to uniquely identifiable objects and their virtual representations in an Internet-like structure to be managed and inventoried by computers. Radio-frequency identification (RFID) - a prerequisite for the IoT - is an automatic way for data transaction in object identification and is used to improve automation, inventory control and checkout operations. An RFID system consists of a reader device and one or several tags. Smart reader systems are...

  13. Implementation and extension of a GNU-Radio RFID reader

    Science.gov (United States)

    Smietanka, G.; Brato, S.; Freudenberg, M.; Götze, J.

    2013-07-01

    The development of a flexible software defined RFID is discused. Commercial reader systems only allow a top level view on the communication and restrict the variation for many transmission parameters. Recently a software reader from the CGran project was proposed which uses the GNU Radio environment in combination with an USRP front end. Because most of the signal processing is done on a common host PC, this reader offers high flexibility, but also has several disadvantages. One of the main hardware limitations is the usage of only one separated antenna per transmit and receive path. Commercial readers usually use four antennas which are time multiplexed and can be used as transmitter and receiver. In this work a HF multiplexer for the USRP device is introduced. With this extension up to four transmit and receive antennas can be used in combination with the software reader. It is shown that the multiplexer achieves good read rates for a switching interval of 100 ms. Using this multiplexer the read range of the system decrease compared to the basic software reader, but distances over two meters can still be realized without additional hardware extensions.

  14. RFID Reader Anticollision Protocols for Dense and Mobile Deployments

    Directory of Open Access Journals (Sweden)

    Abdoul Aziz Mbacke

    2016-11-01

    Full Text Available The rapid development of RFID (Radio Frequency IDentification technology has allowed its large adoption and led to increasing deployments of RFID solutions in diverse environments under varying scenarios and constraints. The nature of these constraints ranges from the amount to the mobility of the readers deployed, which in turn highly affects the quality of the RFID system, causing reading collisions. Although several solutions were proposed to engage the issue of reading collision, few were ever concerned with the densification and/or mobility of readers. This paper proposes two distributed TDMA (Time Division Multiple Access approaches designed to reduce these collisions through local coordination between neighboring devices for different scenarios tested here. The first proposal is based on a reservation phase organized between readers with different priority levels given to readers depending on their previous success. The second one takes advantage of the particular case of RFID collisions, allowing a local and mutual decision of each reader to access or not tags in their vicinity. Simulations were run over different stressful environments in terms of tag/reader density and mobility, proving that our proposals achieved the best performance in terms of throughput, collision avoidance and coverage delay when compared to other collision reducing schemes.

  15. An IoT Reader for Wireless Passive Electromagnetic Sensors.

    Science.gov (United States)

    Galindo-Romera, Gabriel; Carnerero-Cano, Javier; Martínez-Martínez, José Juan; Herraiz-Martínez, Francisco Javier

    2017-03-28

    In the last years, many passive electromagnetic sensors have been reported. Some of these sensors are used for measuring harmful substances. Moreover, the response of these sensors is usually obtained with laboratory equipment. This approach highly increases the total cost and complexity of the sensing system. In this work, a novel low-cost and portable Internet-of-Things (IoT) reader for passive wireless electromagnetic sensors is proposed. The reader is used to interrogate the sensors within a short-range wireless link avoiding the direct contact with the substances under test. The IoT functionalities of the reader allows remote sensing from computers and handheld devices. For that purpose, the proposed design is based on four functional layers: the radiating layer, the RF interface, the IoT mini-computer and the power unit. In this paper a demonstrator of the proposed reader is designed and manufactured. The demonstrator shows, through the remote measurement of different substances, that the proposed system can estimate the dielectric permittivity. It has been demonstrated that a linear approximation with a small error can be extracted from the reader measurements. It is remarkable that the proposed reader can be used with other type of electromagnetic sensors, which transduce the magnitude variations in the frequency domain.

  16. Reviews in fluorescence 2010

    CERN Document Server

    Geddes, Chris D

    2011-01-01

    ""Reviews in Fluorescence 2010"", the seventh volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the year's progress in fluorescence and its applications, with authoritative analytical reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. ""Reviews in Fluorescence"" offers an essential reference material for any lab working in the fluoresc

  17. Principles of fluorescence techniques

    CERN Document Server

    2016-01-01

    Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies.

  18. MyPlate Food Guide

    Science.gov (United States)

    ... Choosing the Right Sport for You Shyness MyPlate Food Guide KidsHealth > For Teens > MyPlate Food Guide Print ... other sugary drinks. Avoid oversized portions. continue Five Food Groups Different food groups meet different nutrition needs. ...

  19. What Are Growth Plate Injuries?

    Science.gov (United States)

    ... plate injuries are:  Falling down  Competitive sports (like football)  Recreational activities. Other reasons for growth plate injuries are:  Child abuse  Injury from extreme cold (for ...

  20. Networks of reader and country status: an analysis of Mendeley reader statistics

    Directory of Open Access Journals (Sweden)

    Robin Haunschild

    2015-11-01

    Full Text Available The number of papers published in journals indexed by the Web of Science core collection is steadily increasing. In recent years, nearly two million new papers were published each year; somewhat more than one million papers when primary research papers are considered only (articles and reviews are the document types where primary research is usually reported or reviewed. However, who reads these papers? More precisely, which groups of researchers from which (self-assigned scientific disciplines and countries are reading these papers? Is it possible to visualize readership patterns for certain countries, scientific disciplines, or academic status groups? One popular method to answer these questions is a network analysis. In this study, we analyze Mendeley readership data of a set of 1,133,224 articles and 64,960 reviews with publication year 2012 to generate three different networks: (1 The network based on disciplinary affiliations of Mendeley readers contains four groups: (i biology, (ii social sciences and humanities (including relevant computer sciences, (iii bio-medical sciences, and (iv natural sciences and engineering. In all four groups, the category with the addition “miscellaneous” prevails. (2 The network of co-readers in terms of professional status shows that a common interest in papers is mainly shared among PhD students, Master’s students, and postdocs. (3 The country network focusses on global readership patterns: a group of 53 nations is identified as core to the scientific enterprise, including Russia and China as well as two thirds of the OECD (Organisation for Economic Co-operation and Development countries.

  1. Controlling Laminate Plate Elastic Behavior

    OpenAIRE

    Mareš, T.

    2004-01-01

    This paper aims to express the relation of a measure of laminate plate stiffness with respect to the fiber orientation of its plies. The inverse of the scalar product of the lateral displacement of the central plane and lateral loading of the plate is the measure of laminate plate stiffness. In the case of a simply supported rectangular laminate plate this measure of stiffness is maximized, and the optimum orientation of its plies is searched.

  2. Inferential processes in readers with Down syndrome

    Directory of Open Access Journals (Sweden)

    Rosende-Vázquez, Marta

    2013-03-01

    Full Text Available The aim of this work is to know if the source of the difficulty in making inferences, readers with Down syndrome, is in access to prior knowledge or constructing ideas from purely textual knowledge (based on Saldaña and Frith, 2002 for autism. Involved a sample of 20 students with Down syndrome and mild mental retardation (mean IQ = 60 and a control group of 20 children without cognitive deficits. They were matched as to their extent read metal age via Prueba de Evaluación del Retraso Lector (average 8 years. We created two experimental situations: a subjects had to generate inferences based on physical knowledge, b social inferences about knowledge. The ability to check and reaction times in the activation of inferences about physical and social knowledge. We also analyzed the influence that the effect "priming". Results showed: a a rate of correct inferences similar verification tasks between the two groups, b Down subjects take longer to access knowledge that the previous text, c reaction times used by subjects Down were higher in activating physical inferences, d there were no significant differences in the population without reaction times gap between physical and social inferences e subjects without deficits benefited effect "priming" in both types of inferences f Down subjects only improve reaction time in the inferences of social nature. El presente estudio pretende conocer si el origen de la dificultad para realizar inferencias, en lectores con Síndrome de Down, se encuentra en el acceso al conocimiento previo o en la construcción de ideas a partir del conocimiento puramente textual (basándonos en Saldaña y Frith, 2002 para autismo. Participó una muestra de 20 alumnos con Síndrome de Down y discapacidad mental leve (media de C.I.= 60 y un grupo control de 20 alumnos sin déficit cognitivo. Ambos fueron igualados en cuanto a su edad mental lectora medida a través de la Prueba de Evaluación del Retraso Lector (media 8 a

  3. Microchannel plate streak camera

    Science.gov (United States)

    Wang, Ching L.

    1989-01-01

    An improved streak camera in which a microchannel plate electron multiplier is used in place of or in combination with the photocathode used in prior streak cameras. The improved streak camera is far more sensitive to photons (UV to gamma-rays) than the conventional x-ray streak camera which uses a photocathode. The improved streak camera offers gamma-ray detection with high temporal resolution. It also offers low-energy x-ray detection without attenuation inside the cathode. Using the microchannel plate in the improved camera has resulted in a time resolution of about 150 ps, and has provided a sensitivity sufficient for 1000 KeV x-rays.

  4. Fluorescence correlation spectroscopy

    NARCIS (Netherlands)

    Hink, M.A.; Verveer, P.J.

    2015-01-01

    Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecul

  5. Plate-specific gain map correction for the improvement of detective quantum efficiency in computed radiography

    Energy Technology Data Exchange (ETDEWEB)

    Schnell, Erich A.; Samei, Ehsan; Dobbins, James T. [Ravin Advanced Imaging Laboratories, Department of Radiology and Medical Physics Graduate Program, Duke University Medical Center, North Carolina 27705 (United States); Ravin Advanced Imaging Laboratories, Departments of Radiology, Biomedical Engineering, and Physics, and Medical Physics Graduate Program, Duke University Medical Center, North Carolina 27705 (United States)

    2012-03-15

    Purpose: The purpose of this work is to improve the noise power spectrum (NPS), and thus the detective quantum efficiency (DQE), of computed radiography (CR) images by correcting for spatial gain variations specific to individual imaging plates. CR devices have not traditionally employed gain-map corrections, unlike the case with flat-panel detectors, because of the multiplicity of plates used with each reader. The lack of gain-map correction has limited the DQE(f) at higher exposures with CR. This current work describes a feasible solution to generating plate-specific gain maps. Methods: Ten high-exposure open field images were taken with an RQA5 spectrum, using a sixth generation CR plate suspended in air without a cassette. Image values were converted to exposure, the plates registered using fiducial dots on the plate, the ten images averaged, and then high-pass filtered to remove low frequency contributions from field inhomogeneity. A gain-map was then produced by converting all pixel values in the average into fractions with mean of one. The resultant gain-map of the plate was used to normalize subsequent single images to correct for spatial gain fluctuation. To validate performance, the normalized NPS (NNPS) for all images was calculated both with and without the gain-map correction. Variations in the quality of correction due to exposure levels, beam voltage/spectrum, CR reader used, and registration were investigated. Results: The NNPS with plate-specific gain-map correction showed improvement over the noncorrected case over the range of frequencies from 0.15 to 2.5 mm{sup -1}. At high exposure (40 mR), NNPS was 50%-90% better with gain-map correction than without. A small further improvement in NNPS was seen from carefully registering the gain-map with subsequent images using small fiducial dots, because of slight misregistration during scanning. Further improvement was seen in the NNPS from scaling the gain map about the mean to account for different beam

  6. Plate-specific gain map correction for the improvement of detective quantum efficiency in computed radiography.

    Science.gov (United States)

    Schnell, Erich A; Samei, Ehsan; Dobbins, James T

    2012-03-01

    The purpose of this work is to improve the noise power spectrum (NPS), and thus the detective quantum efficiency (DQE), of computed radiography (CR) images by correcting for spatial gain variations specific to individual imaging plates. CR devices have not traditionally employed gain-map corrections, unlike the case with flat-panel detectors, because of the multiplicity of plates used with each reader. The lack of gain-map correction has limited the DQE(f) at higher exposures with CR. This current work describes a feasible solution to generating plate-specific gain maps. Ten high-exposure open field images were taken with an RQA5 spectrum, using a sixth generation CR plate suspended in air without a cassette. Image values were converted to exposure, the plates registered using fiducial dots on the plate, the ten images averaged, and then high-pass filtered to remove low frequency contributions from field inhomogeneity. A gain-map was then produced by converting all pixel values in the average into fractions with mean of one. The resultant gain-map of the plate was used to normalize subsequent single images to correct for spatial gain fluctuation. To validate performance, the normalized NPS (NNPS) for all images was calculated both with and without the gain-map correction. Variations in the quality of correction due to exposure levels, beam voltage/spectrum, CR reader used, and registration were investigated. The NNPS with plate-specific gain-map correction showed improvement over the noncorrected case over the range of frequencies from 0.15 to 2.5 mm(-1). At high exposure (40 mR), NNPS was 50%-90% better with gain-map correction than without. A small further improvement in NNPS was seen from carefully registering the gain-map with subsequent images using small fiducial dots, because of slight misregistration during scanning. Further improvement was seen in the NNPS from scaling the gain map about the mean to account for different beam spectra. This study

  7. Electronic Equipment Cold Plates

    Science.gov (United States)

    1976-04-01

    equations for such a flow regiae. For laainar flow and Moderate teaperature differwwe« between the well «nd coolant, a aodifled Sieder -Tate...con- figuration. The heat-transfer coefficients, therefore, were determined by using both the Sieder -Tate and McAdams equations and the coaputed...values used In the analytical predictions. As with th* previous cold Plates, the Sieder -Tate equation gave too low of values for the heat- transfer

  8. Elastic plate spallation

    Science.gov (United States)

    Oline, L.; Medaglia, J.

    1972-01-01

    The dynamic finite element method was used to investigate elastic stress waves in a plate. Strain displacement and stress strain relations are discussed along with the stiffness and mass matrix. The results of studying point load, and distributed load over small, intermediate, and large radii are reported. The derivation of finite element matrices, and the derivation of lumped and consistent matrices for one dimensional problems with Laplace transfer solutions are included. The computer program JMMSPALL is also included.

  9. Plate Tearing by a Cone

    DEFF Research Database (Denmark)

    Simonsen, Bo Cerup

    1997-01-01

    The present paper is concerned with steady-state plate tearing by a cone. This is a scenario where a cone is forced through a ductile metal plate with a constant lateral tip penetration in a motion in the plane of the plate. The considered process could be an idealisaton of the damage, which...

  10. Plate Tearing by a Cone

    DEFF Research Database (Denmark)

    Simonsen, Bo Cerup

    1998-01-01

    The present paper is concerned with steady-state plate tearing by a cone. This is a scenario where a cone is forced through a ductile metal plate with a constant lateral tip penetration in a motion in the plane of the plate. The considered process could be an idealisation of the damage, which...

  11. Safe biodegradable fluorescent particles

    Science.gov (United States)

    Martin, Sue I.; Fergenson, David P.; Srivastava, Abneesh; Bogan, Michael J.; Riot, Vincent J.; Frank, Matthias

    2010-08-24

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  12. Visual consequences of electronic reader use: a pilot study.

    Science.gov (United States)

    Maducdoc, Marlon M; Haider, Asghar; Nalbandian, Angèle; Youm, Julie H; Morgan, Payam V; Crow, Robert W

    2017-04-01

    With the increasing prevalence of electronic readers (e-readers) for vocational and professional uses, it is important to discover if there are visual consequences in the use of these products. There are no studies in the literature quantifying the incidence or severity of eyestrain, nor are there clinical characteristics that may predispose to these symptoms with e-reader use. The primary objective of this pilot study was to assess the degree of eyestrain associated with e-reader use compared to traditional paper format. The secondary outcomes of this study were to assess the rate of eyestrain associated with e-reader use and identify any clinical characteristics that may be associated with the development of eyestrain. Forty-four students were randomly assigned to study (e-reader iPAD) and control (print) groups. Participant posture, luminosity of the room, and reading distance from reading device were measured during a 1-h session for both groups. At the end of the session, questionnaires were administered to determine symptoms. Significantly higher rates of eyestrain (p = 0.008) and irritation (p = 0.011) were found among the iPAD study group as compared to the print 'control' group. The study group was also 4.9 times more likely to report severe eyestrain (95 % CI [1.4, 16.9]). No clinical characteristics predisposing to eyestrain could be identified. These findings conclude that reading on e-readers may induce increased levels of irritation and eyestrain. Predisposing factors, etiology, and potential remedial interventions remain to be determined.

  13. Comparative performance of a primary-reader and second-reader paradigm of computer-aided detection for CT colonography in a low-prevalence screening population.

    Science.gov (United States)

    Miyake, Mototaka; Iinuma, Gen; Taylor, Stuart A; Halligan, Steve; Morimoto, Tsuyoshi; Ichikawa, Tamaki; Tomimatsu, Hideto; Beddoe, Gareth; Sugimura, Kazuro; Arai, Yasuaki

    2013-05-01

    To compare the efficacy of computer-aided detection (CAD) for computed tomographic colonography (CTC) when employed as either primary-reader or second-reader paradigms in a low-prevalence screening population. Ninety screening patients underwent same-day CTC and colonoscopy. Four readers prospectively interpreted all CTC data sets using a second-reader paradigm (unassisted interpretation followed immediately by CAD assistance). Three months later, randomized anonymous data sets were re-interpreted by all readers using a primary-reader paradigm (only CAD prompts evaluated). Compared with the average per-patient sensitivity for unassisted interpretation (0.57), both CAD paradigms significantly increased sensitivity: 0.78 (p reader paradigm and 0.83 (p reader paradigm. There was no significant difference between CAD paradigms (p = 0.25). The average per-patient specificity for polyps ≥6 mm was significantly higher using the primary-reader paradigm than the second-reader paradigm (0.90 vs. 0.83, respectively, p = 0.006), with ROC AUCs of 0.83 and 0.68, respectively. Reading time using CAD as a primary-reader paradigm (median 1.4 min) was significantly shorter than both unassisted (median 4.0 min, p reader paradigms (median 5.5 min, p reader paradigm, although the latter may improve specificity and efficiency more.

  14. Plate tectonics conserves angular momentum

    Directory of Open Access Journals (Sweden)

    C. Bowin

    2009-03-01

    Full Text Available A new combined understanding of plate tectonics, Earth internal structure, and the role of impulse in deformation of the Earth's crust is presented. Plate accelerations and decelerations have been revealed by iterative filtering of the quaternion history for the Euler poles that define absolute plate motion history for the past 68 million years, and provide an unprecedented precision for plate angular rotation variations with time at 2-million year intervals. Stage poles represent the angular rotation of a plate's motion between adjacent Euler poles, and from which the maximum velocity vector for a plate can be determined. The consistent maximum velocity variations, in turn, yield consistent estimates of plate accelerations and decelerations. The fact that the Pacific plate was shown to accelerate and decelerate, implied that conservation of plate tectonic angular momentum must be globally conserved, and that is confirmed by the results shown here (total angular momentum ~1.4 E+27 kgm2s−1. Accordingly, if a plate decelerates, other plates must increase their angular momentums to compensate. In addition, the azimuth of the maximum velocity vectors yields clues as to why the "bend" in the Emperor-Hawaiian seamount trend occurred near 46 Myr. This report summarizes processing results for 12 of the 14 major tectonic plates of the Earth (except for the Juan de Fuca and Philippine plates. Plate accelerations support the contention that plate tectonics is a product of torques that most likely are sustained by the sinking of positive density anomalies due to phase changes in subducted gabbroic lithosphere at depth in the upper lower mantle (above 1200 km depth. The tectonic plates are pulled along by the sinking of these positive mass anomalies, rather than moving at near constant velocity on the crests of convection cells driven by rising heat. These results imply that spreading centers are primarily passive reactive

  15. Vehicle License Plate Recognition Syst

    Directory of Open Access Journals (Sweden)

    Meenakshi,R. B. Dubey

    2012-12-01

    Full Text Available The vehicle license plate recognition system has greater efficiency for vehicle monitoring in automatic zone access control. This Plate recognition system will avoid special tags, since all vehicles possess a unique registration number plate. A number of techniques have been used for car plate characters recognition. This system uses neural network character recognition and pattern matching of characters as two character recognition techniques. In this approach multilayer feed-forward back-propagation algorithm is used. The performance of the proposed algorithm has been tested on several car plates and provides very satisfactory results.

  16. Engaging Literacy: A Case Study in the Use of e-Reader Devices

    Science.gov (United States)

    Lettenmaier, Kris

    2013-01-01

    The purpose of this case study was to determine to what extent e-readers affected struggling readers as a part of reading instruction at the middle school level to improve students' literacy skills of comprehension, fluency, and vocabulary, and to show how e-readers affected engagement and self-efficacy of struggling readers to read and use…

  17. Cognitive Processes in Discourse Comprehension: Passive Processes, Reader-Initiated Processes, and Evolving Mental Representations

    Science.gov (United States)

    van den Broek, Paul; Helder, Anne

    2017-01-01

    As readers move through a text, they engage in various types of processes that, if all goes well, result in a mental representation that captures their interpretation of the text. With each new text segment the reader engages in passive and, at times, reader-initiated processes. These processes are strongly influenced by the readers'…

  18. Using Twitter to Teach Reader-Oriented Biblical Interpretation: "Tweading" the Gospel of Mark

    Science.gov (United States)

    Williamson, Robert, Jr.

    2013-01-01

    Twitter offers an engaging way to introduce students to reader-oriented interpretation of the Bible. The exercise described here introduces students to the idea that the reader has a role in the production of a text's meaning, which thus varies from reader to reader. Twitter enables us to capture the real-time thoughts of a variety of…

  19. Using plate mapping to examine portion size and plate composition for large and small divided plates.

    Science.gov (United States)

    Sharp, David E; Sobal, Jeffery; Wansink, Brian

    2014-12-01

    Does the size of a plate influence the serving of all items equally, or does it influence the serving of some foods - such as meat versus vegetables - differently? To examine this question, we used the new method of plate mapping, where people drew a meal on a paper plate to examine sensitivity to small versus large three-compartment divided plates in portion size and meal composition in a sample of 109 university students. The total drawn meal area was 37% bigger on large plates than small plates, which showed that the portion of plate coverage did not differ by plate size. Men and women drew bigger vegetable portions and men drew bigger meat portions on large plates when compared to small plates. These results suggest that men and women are differentially sensitive to plate size for overall meal size and for meal composition. Implications for decreasing portion size and improving meal balance are that plate size may influence portion size and change the proportions of foods served.

  20. A Redox-Activatable Fluorescent Sensor for the High-Throughput Quantification of Cytosolic Delivery of Macromolecules.

    Science.gov (United States)

    Wang, Zhaohui; Luo, Min; Mao, Chengqiong; Wei, Qi; Zhao, Tian; Li, Yang; Huang, Gang; Gao, Jinming

    2017-01-24

    Efficient delivery of biomacromolecules (e.g., proteins, nucleic acids) into cell cytosol remains a critical challenge for the development of macromolecular therapeutics or diagnostics. To date, most common approaches to assess cytosolic delivery rely on fluorescent labeling of macromolecules with an "always on" reporter and subcellular imaging of endolysosomal escape by confocal microscopy. This strategy is limited by poor signal-to-noise ratio and only offers low throughput, qualitative information. Herein we describe a quantitative redox-activatable sensor (qRAS) for the real-time monitoring of cytosolic delivery of macromolecules. qRAS-labeled macromolecules are silent (off) inside the intact endocytic organelles, but can be turned on by redox activation after endolysosomal disruption and delivery into the cytosol, thereby greatly improving the detection accuracy. In addition to confocal microscopy, this quantitative sensing technology allowed for a high-throughput screening of a panel of polymer carriers toward efficient cytosolic delivery of model proteins on a plate reader. The simple and versatile qRAS design offers a useful tool for the investigation of new strategies for endolysosomal escape of biomacromolecules to facilitate the development of macromolecular therapeutics for a variety of disease indications. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. A Novel Automatic Rapid Diagnostic Test Reader Platform.

    Science.gov (United States)

    Ozkan, Haydar; Kayhan, Osman Semih

    2016-01-01

    A novel automatic Rapid Diagnostic Test (RDT) reader platform is designed to analyze and diagnose target disease by using existing consumer cameras of a laptop-computer or a tablet. The RDT reader is useable with numerous lateral immunochromatographic assays and similar biomedical tests. The system has two different components, which are 3D-printed, low-cost, tiny, and compact stand and a decision program named RDT-AutoReader 2.0. The program takes the image of RDT, crops the region of interest (ROI), and extracts the features from the control end test lines to classify the results as invalid, positive, or negative. All related patient's personal information, image of ROI, and the e-report are digitally saved and transferred to the related clinician. Condition of the patient and the progress of the disease can be monitored by using the saved data. The reader platform has been tested by taking image from used cassette RDTs of rotavirus (RtV)/adenovirus (AdV) and lateral flow strip RDTs of Helicobacter pylori (H. pylori) before discarding them. The created RDT reader can also supply real-time statistics of various illnesses by using databases and Internet. This can help to inhibit propagation of contagious diseases and to increase readiness against epidemic diseases worldwide.

  2. CT colonography: effect of experience and training on reader performance

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, Stuart A.; Burling, David; Morley, Simon; Bartram, Clive I. [Department of Intestinal Imaging, St. Mark' s Hospital, Watford Road, Northwick Park, HA1 3UJ, London (United Kingdom); Halligan, Steve [Department of Intestinal Imaging, St. Mark' s Hospital, Watford Road, Northwick Park, HA1 3UJ, London (United Kingdom); Department of Cancer Research UK Colorectal Cancer Unit, St. Mark' s Hospital, Northwick Park, HA1 3UJ, London (United Kingdom); Bassett, Paul [Department of Statistics, St. Mark' s Hospital, Northwick Park, HA1 3UJ, London (United Kingdom); Atkin, Wendy [Department of Cancer Research UK Colorectal Cancer Unit, St. Mark' s Hospital, Northwick Park, HA1 3UJ, London (United Kingdom)

    2004-06-01

    The purpose of this paper was to investigate the effect of radiologist experience and increasing exposure to CT colonography on reader performance. Three radiologists of differing general experience (consultant, research fellow, trainee) independently analysed 100 CT colonographic datasets. Readers had no prior experience of CT colonography and received feedback and training after the first 50 cases from an independent experienced radiologist. Diagnostic performance and reporting times were compared for the first and second 50 datasets and compared with the results of a radiologist experienced in CT colonography. Before training only the consultant reader achieved statistical equivalence with the reference standard for detection of larger polyps. After training, detection rates ranged between 25 and 58% for larger polyps. Only the trainee significantly improved after training (P=0.007), with performance of other readers unchanged or even worse. Reporting times following training were reduced significantly for the consultant and fellow (P<0.001 and P=0.03, respectively), but increased for the trainee (P<0.001). In comparison to the consultant reader, the odds of detection of larger polyps was 0.36 (CI 0.16, 0.82) for the fellow and 0.36 (CI 0.14, 0.91) for the trainee. There is considerable variation in the ability to report CT colonography. Prior experience in gastrointestinal radiology is a distinct advantage. Competence cannot be assumed even after directed training via a database of 50 cases. (orig.)

  3. Temperature field of steel plate cooling process after plate rolling

    Directory of Open Access Journals (Sweden)

    Huijun Feng, Lingen Chen, Fengrui Sun

    2015-01-01

    Full Text Available Based on numerical calculation with Matlab, the study on cooling process after plate rolling is carried out, and the temperature field distribution of the plate varying with the time is obtained. The effects of the plate thickness, final rolling temperature, cooling water temperature, average flow rate of the cooling water, carbon content of the plate and cooling method on the plate surface and central temperatures as well as final cooling temperature are discussed. For the same cooling time, the plate surface and central temperatures as well as their temperature difference increase; with the decrease in rolling temperature and the increase in average flow rate of the cooling water, the plate surface and central temperatures decrease. Compared with the single water cooling process, the temperature difference between the plate centre and surface based on intermittent cooling is lower. In this case, the temperature uniformity of the plate is better, and the corresponding thermal stress is lower. The fitting equation of the final cooling temperature with respect to plate thickness, final rolling temperature, cooling water temperature and average flow rate of the cooling water is obtained.

  4. Real Plates and Dubious Microplates

    Science.gov (United States)

    Kogan, M. G.; Steblov, G. M.

    2008-12-01

    From the onset of plate tectonics, the existence of most of the plates was never put in doubt, although the boundaries of some plates, like Africa, were later revised. There are however, two microplates in northeast Asia, the Amurian and Okhotsk, whose existence and the sense of rotation was revised several times. The rms value of plate-residual GPS velocities is 0.5-0.9 mm/a for sets of stations representing the motion of the following plates: Antarctic, Australian, Eurasian, North American, Nubian, Pacific, and South American. This value can be regarded as an upper bound on deviation of real plates from infinite stiffness. The rms value of plate-residual GPS velocities is 1.2-1.8 mm/a for the Indian, Nazca, and Somalian plates. Higher rms values for India and Nazca are attributed to the noisier data. The higher rms value for Somalia appears to arise from the distributed deformation to the east of the East African Rift; whether this statement is true can only be decided from observations of denser network in the future. From the analysis of plate-residual GPS velocities, the Canadian Arctic and northeastern Siberia belong to the North American plate. The detailed GPS survey on Sakhalin Island shows that the Sea of Okhotsk region also belongs to the North American plate while the region to the west of it belongs to the Eurasian plate. These results provide a constraint on the geometry of the North American plate and put in doubt the existence of smaller plates in northeast Asia.

  5. Plate Full of Color

    Centers for Disease Control (CDC) Podcasts

    2008-08-04

    The Eagle Books are a series of four books that are brought to life by wise animal characters - Mr. Eagle, Miss Rabbit, and Coyote - who engage Rain That Dances and his young friends in the joy of physical activity, eating healthy foods, and learning from their elders about health and diabetes prevention. Plate Full of Color teaches the value of eating a variety of colorful and healthy foods.  Created: 8/4/2008 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 8/5/2008.

  6. Dynamics of Tectonic Plates

    CERN Document Server

    Pechersky, E; Sadowski, G; Yambartsev, A

    2014-01-01

    We suggest a model that describes a mutual dynamic of tectonic plates. The dynamic is a sort of stick-slip one which is modeled by a Markov random process. The process defines a microlevel of the dynamic. A macrolevel is obtained by a scaling limit which leads to a system of integro-differential equations which determines a kind of mean field systems. Conditions when Gutenberg-Richter empirical law are presented on the mean field level. These conditions are rather universal and do not depend on features of resistant forces.

  7. Dynamics of Tectonic Plates

    OpenAIRE

    2014-01-01

    We suggest a model that describes a mutual dynamic of tectonic plates. The dynamic is a sort of stick-slip one which is modeled by a Markov random process. The process defines a microlevel of the dynamic. A macrolevel is obtained by a scaling limit which leads to a system of integro-differential equations which determines a kind of mean field systems. Conditions when Gutenberg-Richter empirical law are presented on the mean field level. These conditions are rather universal and do not depend ...

  8. A Difficult Choice for Newspapers: Advertisers or Readers?

    Directory of Open Access Journals (Sweden)

    Barış Bulunmnaz

    2012-03-01

    Full Text Available Almost entire advertisement revenues are acquired by newspapers in print media. Along with rapid technological developments and the intensive competitive climate in newspaper sector; the gulf between high cost and low revenue has widened, and newspapers are obliged to subsidize this deficit with advertisement revenues. Newspapers in Turkey have two separate groups of customers. The first one of those groups is the advertisers and the other is the readers of that newspaper. Due to economic reasons, this compulsory dependency on advertisement revenue causes newspapers to fall into a paradox between advertisers and readers. In this article we will firstly explain advertisement and its place in marketing; then give information about the advertising sector and the distribution of Turkish advertising pie among media. Later, the relation between advertisers and readers that place newspapers in difficult circumstances will be explicated in detail, analysis and assessments will be made regarding about it.

  9. Reader's Advisory and Bibliotherapy:Helping or Healing?

    Directory of Open Access Journals (Sweden)

    Brian W. Sturm

    2003-12-01

    Full Text Available Reader s advisory, helping library patrons find books to read based on their prior reading preferences, is a common endeavor for most librarians. Bibliotherapy, using books to promote healing, is a special kind of reader s advisory. This article traces the origins of these two concepts and examines their underlying assumptions. It addresses the process through which stories may aid in healing and the process librarians should follow if they decide to engage in bibliotherapy. It concludes that librarians must know the difference between advising and counseling on both a professional and personal level and that they should be wary of letting the power inherent in the readers advisory role endanger their professionalism.

  10. When high-capacity readers slow down and low-capacity readers speed up: Working memory and locality effects

    Directory of Open Access Journals (Sweden)

    Bruno eNicenboim

    2016-03-01

    Full Text Available We examined the effects of argument-head distance in SVO and SOV languages (Spanish and German, while taking into account readers’ working memory capacity and controlling for expectation (Levy, 2008 and other factors. We predicted only locality effects, that is, a slow-down produced by increased dependency distance (Gibson, 2000; Lewis & Vasishth, 2005. Furthermore, we expected stronger locality effects for readers with low working memory capacity. Contrary to our predictions, low-capacity readers showed faster reading with increased distance, while high-capacity readers showed locality effects. We suggest that while the locality effects are compatible with memory-based explanations, the speedup of low-capacity readers can be explained by an increased probability of retrieval failure. We present a computational model based on ACT-R built under the previous assumptions, which is able to give a qualitative account for the present data and can be tested in future research. Our results suggest that in some cases, interpreting longer RTs as indexing increased processing difficulty and shorter RTs as facilitation may be too simplistic: The same increase in processing difficulty may lead to slowdowns in high-capacity readers and speedups in low-capacity ones. Ignoring individual level capacity differences when investigating locality effects may lead to misleading conclusions.

  11. A study on the development of an infographic reader questionnaire and reader opinions

    Directory of Open Access Journals (Sweden)

    Yıldırım Serkan

    2016-01-01

    Full Text Available Educational materials are commonly used to assist with communication during learning. One of the ways to determine the use of educational materials and their contribution to the learning process is to ask students for their opinions. Student opinions provide useful information regarding various objectives such as making both the learning process and resources more effective, bringing about the necessary improvements and organising the teaching process. In this study, we developed a questionnaire for determining the opinions of information graphics (infographics readers on information graphics prepared for teaching purposes. This study was conducted in the fall semester of the 2013-2014 academic year with the participation of 59 students from the Department of Computer and Instructional Technologies Education of the Kazım Karabekir Faculty of Education. The students were provided with a questionnaire consisting of 29 items that inquired their opinions on infographics. The collected data were assessed using Principal Component Analysis. Based on the analysis results, a second questionnaire consisting of 23 items and six factors (Learnability, Selection Preferences, Sharing, Basic Presentation Structure, Memorability, and Role in the Learning Process was developed. According to the study results, the students considered infographics as highly instructive, and described that they help ensure a more lasting learning experience.

  12. E-Books on the Mobile E-Reader

    OpenAIRE

    Chulyun Kim; Ok-Ran Jeong; Jaehyuk Choi; Won Kim

    2013-01-01

    The market for e-books has been established during the past several years. Many e-book readers are commercially available, and millions of e-book titles are available for purchase or free download. E-paper technology has matured enough to be used as the screen for dedicated e-book readers which make reading possible with the familiar feel of reading printed books. In this paper, we provide a comprehensive review of the status of the e-book, including the architecture and features of e-book re...

  13. Optical benchmarking of security document readers for automated border control

    Science.gov (United States)

    Valentín, Kristián.; Wild, Peter; Å tolc, Svorad; Daubner, Franz; Clabian, Markus

    2016-10-01

    Authentication and optical verification of travel documents upon crossing borders is of utmost importance for national security. Understanding the workflow and different approaches to ICAO 9303 travel document scanning in passport readers, as well as highlighting normalization issues and designing new methods to achieve better harmonization across inspection devices are key steps for the development of more effective and efficient next- generation passport inspection. This paper presents a survey of state-of-the-art document inspection systems, showcasing results of a document reader challenge investigating 9 devices with regards to optical characteristics.

  14. Hand-held portable microarray reader for biodetection

    Science.gov (United States)

    Thompson, Deanna Lynn; Coleman, Matthew A; Lane, Stephen M; Matthews, Dennis L; Albala, Joanna; Wachsmann-Hogiu, Sebastian

    2013-04-23

    A hand-held portable microarray reader for biodetection includes a microarray reader engineered to be small enough for portable applications. The invention includes a high-powered light-emitting diode that emits excitation light, an excitation filter positioned to receive the excitation light, a slide, a slide holder assembly for positioning the slide to receive the excitation light from the excitation filter, an emission filter positioned to receive the excitation light from the slide, a lens positioned to receive the excitation light from the emission filter, and a CCD camera positioned to receive the excitation light from the lens.

  15. CMS Resistive plate Champers

    CERN Document Server

    Zainab, Karam

    2013-01-01

    There are many types of gas detectors which are used in CERN in LHC project, There is a main parts for the gas detectors which must be in all gas detectors types like Multiwire proportional chambers, such as the micromesh gaseous structure chamber (the MicroMegas), Gas-electron multiplier (GEM) detector, Resistive Plate Champers... Compact Muon Solenoid (CMS) experiment detecting muons which are powerful tool for recognizing signatures of interesting physics processes. The CMS detector uses: drift tube (DT), cathode strip chamber (CSC) and resistive plate chamber (RPC). Building RPC’s was my project in summer student program (hardware). RPC’s have advantages which are triggering detector and Excellent time resolution which reinforce the measurement of the correct beam crossing time. RPC’s Organized in stations :  RPC barrel (RB) there are 4 stations, namely RB1, RB2, RB3, and RB4  While in the RPC endcap (RE) the 3 stations are RE1, RE2, and RE3. In the endcaps a new starion will be added and this...

  16. Improving the Sensitivity and Functionality of Mobile Webcam-Based Fluorescence Detectors for Point-of-Care Diagnostics in Global Health

    Directory of Open Access Journals (Sweden)

    Reuven Rasooly

    2016-05-01

    Full Text Available Resource-poor countries and regions require effective, low-cost diagnostic devices for accurate identification and diagnosis of health conditions. Optical detection technologies used for many types of biological and clinical analysis can play a significant role in addressing this need, but must be sufficiently affordable and portable for use in global health settings. Most current clinical optical imaging technologies are accurate and sensitive, but also expensive and difficult to adapt for use in these settings. These challenges can be mitigated by taking advantage of affordable consumer electronics mobile devices such as webcams, mobile phones, charge-coupled device (CCD cameras, lasers, and LEDs. Low-cost, portable multi-wavelength fluorescence plate readers have been developed for many applications including detection of microbial toxins such as C. Botulinum A neurotoxin, Shiga toxin, and S. aureus enterotoxin B (SEB, and flow cytometry has been used to detect very low cell concentrations. However, the relatively low sensitivities of these devices limit their clinical utility. We have developed several approaches to improve their sensitivity presented here for webcam based fluorescence detectors, including (1 image stacking to improve signal-to-noise ratios; (2 lasers to enable fluorescence excitation for flow cytometry; and (3 streak imaging to capture the trajectory of a single cell, enabling imaging sensors with high noise levels to detect rare cell events. These approaches can also help to overcome some of the limitations of other low-cost optical detection technologies such as CCD or phone-based detectors (like high noise levels or low sensitivities, and provide for their use in low-cost medical diagnostics in resource-poor settings.

  17. Localised Plate Motion on Venus

    Science.gov (United States)

    Ghail, R. C.

    1996-03-01

    The volcanic and tectonic features observed in Dali Vinculum, Parga Vinculum and Imdr Regio are concentrated at long, narrow, curvilinear zones, with relatively minor volcanism and tectonism between these zones. These zones, whilst more diffuse than terrestrial plate boundaries, nevertheless define the margins of tectonic plates. In contrast to Earth, however, it appears that venusian plates are neither created nor destroyed by lateral motion. Rather, plates are thinned and intruded at vincula plate boundaries, vertically accreted by small-scale intra-plate (planitia) volcanism and perhaps destroyed by delamination of thickened crust in tesserae and montane regions such as Thetis Regio and Ishtar Terra. The diversity in age both between and within these three areas together with the evidence for infrequent, small scale resurfacing in the planitiae are difficult to reconcile with a non-uniformitarian geological process.

  18. Tectonics of the Easter plate

    Science.gov (United States)

    Engeln, J. F.; Stein, S.

    1984-01-01

    A new model for the Easter plate is presented in which rift propagation has resulted in the formation of a rigid plate between the propagating and dying ridges. The distribution of earthquakes, eleven new focal mechanisms, and existing bathymetric and magnetic data are used to describe the tectonics of this area. Both the Easter-Nazca and Easter-Pacific Euler poles are sufficiently close to the Easter plate to cause rapid changes in rates and directions of motion along the boundaries. The east and west boundaries are propagating and dying ridges; the southwest boundary is a slow-spreading ridge and the northern boundary is a complex zone of convergent and transform motion. The Easter plate may reflect the tectonics of rift propagation on a large scale, where rigid plate tectonics requires boundary reorientation. Simple schematic models to illustrate the general features and processes which occur at plates resulting from large-scale rift propagation are used.

  19. Tectonics of the Easter plate

    Science.gov (United States)

    Engeln, J. F.; Stein, S.

    1984-01-01

    A new model for the Easter plate is presented in which rift propagation has resulted in the formation of a rigid plate between the propagating and dying ridges. The distribution of earthquakes, eleven new focal mechanisms, and existing bathymetric and magnetic data are used to describe the tectonics of this area. Both the Easter-Nazca and Easter-Pacific Euler poles are sufficiently close to the Easter plate to cause rapid changes in rates and directions of motion along the boundaries. The east and west boundaries are propagating and dying ridges; the southwest boundary is a slow-spreading ridge and the northern boundary is a complex zone of convergent and transform motion. The Easter plate may reflect the tectonics of rift propagation on a large scale, where rigid plate tectonics requires boundary reorientation. Simple schematic models to illustrate the general features and processes which occur at plates resulting from large-scale rift propagation are used.

  20. Global analysis of fluorescence decays to probe the internal dynamics of fluorescently labeled macromolecules.

    Science.gov (United States)

    Duhamel, Jean

    2014-03-11

    The aim of this review is to introduce the reader first to the mathematical complexity associated with the analysis of fluorescence decays acquired with solutions of macromolecules labeled with a fluorophore and its quencher that are capable of interacting with each other via photophysical processes within the macromolecular volume, second to the experimental and mathematical approaches that have been proposed over the years to handle this mathematical complexity, and third to the information that one can expect to retrieve with respect to the internal dynamics of such fluorescently labeled macromolecules. In my view, the ideal fluorophore-quencher pair to use in studying the internal dynamics of fluorescently labeled macromolecules would involve a long-lived fluorophore, a fluorophore and a quencher that do not undergo energy migration, and a photophysical process that results in a change in fluorophore emission upon contact between the excited fluorophore and quencher. Pyrene, with its ability to form an excimer on contact between excited-state and ground-state species, happens to possess all of these properties. Although the concepts described in this review apply to any fluorophore and quencher pair sharing pyrene's exceptional photophysical properties, this review focuses on the study of pyrene-labeled macromolecules that have been characterized in great detail over the past 40 years and presents the main models that are being used today to analyze the fluorescence decays of pyrene-labeled macromolecules reliably. These models are based on Birks' scheme, the DMD model, the fluorescence blob model, and the model free analysis. The review also provides a step-by-step protocol that should enable the noneducated user to achieve a successful decay analysis exempt of artifacts. Finally, some examples of studies of pyrene-labeled macromolecules are also presented to illustrate the different types of information that can be retrieved from these fluorescence decay

  1. Aluminum Manganese Molten Salt Plating

    Science.gov (United States)

    2006-06-01

    Dry fixture thoroughly with the air gun. Be especially careful to dry water out of crevices. Note: water is a contaminant to the plating process...easily destroyed if blown with the air. Be especially careful to dry water out of crevices. Note: water is a contaminant to the plating process and...especially careful to dry water out of crevices. 13. Carefully remove part from fixture. If residual plating solution is present at attachments points

  2. Cellphone-based hand-held microplate reader for point-of-care ELISA testing (Conference Presentation)

    Science.gov (United States)

    Berg, Brandon; Cortazar, Bingen; Tseng, Derek; Ozkan, Haydar; Feng, Steve; Wei, Qingshan; Chan, Raymond Y.; Burbano, Jordi; Farooqui, Qamar; Lewinski, Michael; Di Carlo, Dino; Garner, Omai B.; Ozcan, Aydogan

    2016-03-01

    Enzyme-linked immunosorbent assay (ELISA) in a microplate format has been a gold standard first-line clinical test for diagnosis of various diseases including infectious diseases. However, this technology requires a relatively large and expensive multi-well scanning spectrophotometer to read and quantify the signal from each well, hindering its implementation in resource-limited-settings. Here, we demonstrate a cost-effective and handheld smartphone-based colorimetric microplate reader for rapid digitization and quantification of immunoserology-related ELISA tests in a conventional 96-well plate format at the point of care (POC). This device consists of a bundle of 96 optical fibers to collect the transmitted light from each well of the microplate and direct all the transmission signals from the wells onto the camera of the mobile-phone. Captured images are then transmitted to a remote server through a custom-designed app, and both quantitative and qualitative diagnostic results are returned back to the user within ~1 minute per 96-well plate by using a machine learning algorithm. We tested this mobile-phone based micro-plate reader in a clinical microbiology lab using FDA-approved mumps IgG, measles IgG, and herpes simplex virus IgG (HSV-1 and HSV-2) ELISA tests on 1138 remnant patient samples (roughly 50% training and 50% testing), and achieved an overall accuracy of ~99% or higher for each ELISA test. This handheld and cost-effective platform could be immediately useful for large-scale vaccination monitoring in low-infrastructure settings, and also for other high-throughput disease screening applications at POC.

  3. How readers perceive translated literary works: an analysis of reader reception

    Directory of Open Access Journals (Sweden)

    Angela D'Egidio

    2015-12-01

    Full Text Available Abstract - The aim of this paper is to investigate the reader’s reception of translated literary texts and to explore the reader’s expectations about literary works. For this purpose, three comparable corpora of British, American and Italian online book reviews commenting on the English version of Andrea Camilleri’s La forma dell’acqua (The Shape of Water and on the Italian version of Stephen King’s Joyland were assembled and analysed. The way active target language readers perceive and evaluate a translated literary work was then explored by taking the language they actually used in book recommendation websites. The analysis demonstrates that the Anglo-American and Italian readers’ reception of the translated text is different, in terms of focus and perception. Riassunto - Il presente articolo esplora il modo in cui i lettori recepiscono testi letterari tradotti e quali sono le loro aspettative. A tale scopo sono stati raccolti e analizzati tre corpora comparabili di recensioni online scritte da lettori britannici, americani e italiani sulla versione inglese de La forma dell’acqua di Andrea Camilleri e sulla versione italiana di Joyland di Stephen King. In particolare, l’articolo esplora il modo in cui i lettori della lingua di arrivo percepiscono e valutano un’opera letteraria tradotta partendo dal linguaggio da usato dai lettori nei siti web di recensioni di libri. L’analisi dimostra che i lettori anglo-americani e italiani percepiscono un testo tradotto in modo differente e ne commentano aspetti differenti.

  4. Glass-bead peen plating

    Science.gov (United States)

    Graves, J. R.

    1974-01-01

    Peen plating of aluminum, copper, and nickel powders was investigated. Only aluminum was plated successfully within the range of peen plating conditions studied. Optimum plating conditions for aluminum were found to be: (1) bead/powder mixture containing 25 to 35% powder by weight, (2) peening intensity of 0.007A as measured by Almen strip, and (3) glass impact bead diameter of at least 297 microns (0.0117 inches) for depositing-100 mesh aluminum powder. No extensive cleaning or substrate preparation is required beyond removing loose dirt or heavy oil.

  5. High-sensitive and high-efficient biochemical analysis method using a bionic electronic eye in combination with a smartphone-based colorimetric reader system.

    Science.gov (United States)

    Kaiqi Su; Quchao Zou; Ning Hu; Ping Wang

    2015-01-01

    Bionic electronic eye (Bionic e-Eye), a developed smartphone-based colorimetric reader system, consists of smartphone or pad (iPhone 4s or iPad 3) as detection instrument and portable accessory as illumination provider, integrating with a wide-angle lens, a piece of lowest-power electro luminescent and a custom-made dark hood. A 96-well microtiter plate (MTP) was positioned on the electro luminescent and Bionic e-Eye captures the detection images by the back camera of smartphone. Being similar to human visual system, the hue, saturation and value (HSV, also called hex cone model) color model was employed in image processing algorithm of Bionic e-Eye. Optimized system dimension was determined by the system steadiness experiment of different photograph distances. Moreover, the commercially available BCA protein assay and CCK8 cell number assay were carried out to evaluate this Bionic e-Eye. Analytical performance of Bionic e-Eye had the better precision, higher sensitivity than microtiter plate reader (MTPR) and previous smartphone-based colorimetric reader for both two assays. Also, Bionic e-Eye using optical image detection had simultaneous and synchronous working mode, while MPTR using machine moving detection had asynchronous working mode in high throughput detection. Therefore, Bionic e-Eye will be an ideal point-of-care (POC) colorimetric detection device in the field of clinical application, industrial quality control, environment monitoring, and food assessment.

  6. Investigating Readers' Mental Maps of References in an Online System

    Science.gov (United States)

    Yang, Yu-Fen; Wong, Wing-Kwong; Yeh, Hui-Chin

    2009-01-01

    Referential identification and resolution are considered the keys to help readers grasp the main idea of a text and solve lexical ambiguities. The goal of this study is to design a computer system for helping college students who learn English as a Foreign Language (EFL) develop mental maps of referential identification and resolution in reading.…

  7. Manufacturing Descent: Basal Readers and the Creation of Reading Failures.

    Science.gov (United States)

    Shannon, Patrick; Crawford, Patricia

    1997-01-01

    Describes how basal readers manufacture reading failures among students from less-privileged economic and social backgrounds. Reviews the history of reading instruction in the United States. Calls for educators to speak out against reading practices that protect the privilege of the upper and upper-middle classes by encoding their values and…

  8. Medical Readers' Theater: Relevance to Geriatrics Medical Education

    Science.gov (United States)

    Shapiro, Johanna; Cho, Beverly

    2011-01-01

    Medical Readers' Theater (MRT) is an innovative and simple way of helping medical students to reflect on difficult-to-discuss topics in geriatrics medical education, such as aging stereotypes, disability and loss of independence, sexuality, assisted living, relationships with adult children, and end-of-life issues. The authors describe a required…

  9. Readers' Reliance on Source Credibility in the Service of Comprehension

    Science.gov (United States)

    Sparks, Jesse R.; Rapp, David N.

    2011-01-01

    The current project examined the impact of knowledge about the credibility of sources on readers' processing of texts. Participants read texts in which information about characters was provided by either a credible or a noncredible source; this information suggested that the character potentially possessed a particular trait. A subsequent text…

  10. Every Reader a Reviewer: The Online Book Conversation

    Science.gov (United States)

    Hoffert, Barbara

    2010-01-01

    Over the last 15 years, the book review landscape has changed seismically. Reviewing is no longer centralized, with a few big voices leading the way, but fractured among numerous multifarious voices found mostly on the web. In turn, readers aren't playing the captive audience any more. Undone by economics, many traditional print sources have been…

  11. What It Means to Be Strategic: Good Readers as Informants.

    Science.gov (United States)

    Harste, Jerome C.

    A study was conducted to identify strategies used by successful readers in comprehending and interpreting various kinds of texts. Seventy-three graduate students were asked to keep a journal (unedited and freely written) of what they were thinking as they were reading Umberto Eco's novel "The Name of the Rose." Selected journal entries…

  12. Moral Choices in Contemporary Society: A Courses by Newspaper Reader.

    Science.gov (United States)

    Rieff, Philip, Ed.; Finkle, Isaac, Ed.

    This reader, which contains 135 primary source readings about morality, is one of several college-level instructional materials developed to supplement a nationwide newspaper course on moral issues in contemporary society. The authors represent a diverse group including theologians, psychologists, politicians, professional athletes, lawyers, and…

  13. Silly Salamanders and Other Slightly Stupid Stuff for Readers Theatre.

    Science.gov (United States)

    Fredericks, Anthony D.

    This book contains more than two dozen reader's theater scripts to entertain and amuse those in the classroom or library. The scripts in the book--all are reproducible--can help nurture student language arts skills and the power of the imagination with their fractured "takes" on fairy tales and their twisted legends. Designed to…

  14. Reader-Response Theory and the English Curriculum.

    Science.gov (United States)

    Probst, Robert E.

    1994-01-01

    Describes the affinities between reader response theory and the proper objectives of English instruction. Describes students not as potential literary scholars but as curious people needing personal experiences with reading and writing. Outlines a model for teaching literature focused on this conception. (HB)

  15. Children's Response to Literature: Author, Text, Reader, Context.

    Science.gov (United States)

    Sipe, Lawrence R.

    1999-01-01

    Examines how researchers can study children's interaction with literature, discussing various perspectives that can be taken on children's literary responses and suggesting lines of inquiry and concepts that might be useful for the continued examination of children's responses. Focuses on the author, the literary text itself, the reader, and the…

  16. Writer, Reader, Critic: Comparing Critical Theories as Discourse.

    Science.gov (United States)

    de Beaugrande, Robert

    1984-01-01

    Attempts to show how each of three influential critical theories--deconstructionism, reader response criticism, and authorial intention--implies a particular view of how literary discourse is or should be processed and indicates that each view is in part justified, but not to the extent claimed by the critics themselves. (CRH)

  17. What It Means to Be Strategic: Good Readers as Informants.

    Science.gov (United States)

    Harste, Jerome C.

    A study was conducted to identify strategies used by successful readers in comprehending and interpreting various kinds of texts. Seventy-three graduate students were asked to keep a journal (unedited and freely written) of what they were thinking as they were reading Umberto Eco's novel "The Name of the Rose." Selected journal entries were…

  18. AMHARIC BASIC COURSE. UNITS 51-60, READER, GLOSSARY.

    Science.gov (United States)

    OBOLENSKY, SERGE; AND OTHERS

    THE FIRST 10 UNITS OF THIS SECOND VOLUME OF THE AMHARIC BASIC COURSE FOLLOW THE SAME GENERAL FORMAT OF THE PREVIOUS VOLUME IN PRESENTING FURTHER BASIC SENTENCES, DRILLS, AND NARRATIVES. AT THE END OF UNIT 60, AMHARIC ORTHOGRAPHY IS INTRODUCED. SUCCEEDING UNITS, COMPRISING THE READER, ARE WRITTEN IN AMHARIC SCRIPT ONLY. THE GLOSSARY, WHICH IS…

  19. Power Efficient Gurumukhi Unicode Reader Design and Implementation on FPGA

    DEFF Research Database (Denmark)

    Kaur, Amanpreet; Pandey, Bishwajeet; Hussain, Dil Muhammed Akbar

    2017-01-01

    and is implemented on Virtex-6 FPGA on Xilinx software. This GUR design is tested at an different frequencies by applying frequency scaling techniques .The reader is also observed on two different IO Standards i.e. on SSTL (Stub-Series Terminated Logic) and LVDCI (Low Voltage Digitally Controlled Impedance) logic...

  20. The Role of Hand Dominance in Beginning Braille Readers

    Science.gov (United States)

    Wright, Tessa

    2009-01-01

    In this article, the author examines the role of "hand dominance" in beginning braille readers. "Hand dominance" refers to whether an individual is "right handed" or "left handed." The data for these analyses were taken from the Alphabetic Braille and Contracted Braille Study (ABC Braille Study). The ABC Braille Study was a five-year nonrandomized…

  1. ELISA reader does not interfere by mobile phone radiofrequency radiation

    Directory of Open Access Journals (Sweden)

    Seyyed Mohammad Javad Mortazavi

    2016-01-01

    Conclusion: This study showed that ELISA reader does not interfere by mobile phone RF radiation at a closed contact (less than 5 cm distance. However, we recommend that medical institutions discuss these issues in the context of their specific use of technologies and frame a policy that is clear and straightforward to guide staff, patients, and visitors.

  2. How can we motivate students become good readers

    Institute of Scientific and Technical Information of China (English)

    程志航

    2013-01-01

    Reading is one of the four basic skills in learning English. How can teachers motivate primary school students read and help them become good readers? Students are instructed to predict in pre - reading,analysis in while - reading as well as after - reading.

  3. An Experimental Examination of Readers' Perceptions of Media Bias.

    Science.gov (United States)

    D'Alessio, Dave

    2003-01-01

    Explores perceptions of media bias by manipulating expectations of bias and news topic. Explains that university students read dummy newspaper articles and then responded to a survey. Concludes that readers were more likely to designate material opposing their own views as biased. (PM)

  4. How does typeface familiarity affect reading performance and reader preference?

    DEFF Research Database (Denmark)

    Beier, Sofie

    2013-01-01

    Some typographers have proposed that typeface familiarity is defined by the amount of time that a reader has been exposed to a typeface design, while other typographers have proposed that familiarity is defined by the commonalities in letter shapes. These two hypotheses were tested by measuring...

  5. BURIAT READER. URALIC AND ALTAIC SERIES, VOLUME 8.

    Science.gov (United States)

    BOSSON, JAMES E.

    THE INTRODUCTION TO THIS PRIMER-READER CONTAINS A SHORT DESCRIPTION OF BURIAT (A MONGOLIAN LANGUAGE SPOKEN IN THE BURIAT AUTONOMOUS SOVIET SOCIALIST REPUBLIC) AND A BRIEF ACCOUNT OF THE BURIAT CULTURE AND HISTORY. THE BURIAT ORTHOGRAPHY (CYRILLIC-BASED), THE TRANSCRIPTION USED IN THE TEXT, AND VOWEL HARMONY, LENGTH, AND STRESS ARE ALSO TREATED IN…

  6. Suggestopedia: A Teaching Strategy for the Severely Disabled Reader.

    Science.gov (United States)

    James, Bobby M.

    The use of suggestopedia--a technique combining memory expansion and relaxation--as a teaching technique for disabled readers is examined in this paper. The paper first describes the origin of suggestopedia as a therapeutic technique in Bulgaria; it then describes some components of suggestopedia: the creation of physical relaxation, the use of…

  7. The Hands and Reading: What Deafblind Adult Readers Tell Us

    Science.gov (United States)

    Ingraham, Cynthia L.; Andrews, Jean F.

    2010-01-01

    Deafblind readers are heterogeneous in reading skill acquisition. This qualitative study uses in-depth interviews and protocol analyses and queries the three deafblind adult participants in describing their metacomprehension, metacognitive and metalinguistic strategies used when reading different types of text. Using retrospective analysis, the…

  8. Metacomprehension during Basal Reader Instruction: Do Teachers Promote It?

    Science.gov (United States)

    Schmitt, Maribeth Cassidy; Baumann, James F.

    1990-01-01

    Analyzes elementary teachers' interactions with students during guided reading of basal reader selections to determine the extent to which the interactions promote students' metacomprehension abilities. Finds that teachers assumed most of the responsibility for students' comprehension themselves rather than conducting the lessons in a manner that…

  9. Wings of Fancy: Using Readers Theatre to Study Fantasy Genre

    Science.gov (United States)

    Garner, Joan

    2006-01-01

    The new Readers Theatre series features scripts for students in grades 4-8. These original scripts are written for the specific purpose of teaching literary genre. Each book is composed of 24-28 scripts, each of which will be keyed to published books, plays, poems or stories in that genre, encouraging students to read the originals in order to…

  10. Mi Familia (My Family). Chula Vista Readers, Book One.

    Science.gov (United States)

    Van Olst, Ofelia; And Others

    This is book one in a series of beginning readers in Spanish for preschool and grade one. The program is designed to prepare children to read in Spanish by teaching the sound-symbol relationship in sequential order with simple reading selections. This book introduces the consonants "m,""p,""s,""l," and the consonant blend "pl." (Author/AM)

  11. Literary Allusion and Reader Response: Possibilities for Research.

    Science.gov (United States)

    Agee, Hugh

    1983-01-01

    This paper reviews the function of literary allusion and examines how this device helps readers relate to texts. Questions are raised about children's ability to understand allusions, particularly Biblical references. Suggestions for research to improve understanding of the role of allusions are given. (PP)

  12. Empowering Adolescent Readers: Intertextuality in Three Novels by David Almond

    Science.gov (United States)

    Latham, Don

    2008-01-01

    In "Skellig," "Kit's Wilderness," and "Clay", David Almond employs various types of intertextuality to enrich his narratives. Through the use of allusion, adaptation, collage, and mise-en-abyme, he encourages his adolescent readers to seek out precursor texts and to consider the interrelationships between these texts and his own. By so doing, he…

  13. Child Readers and the Worlds of the Picture Book

    Science.gov (United States)

    Baird, Adela; Laugharne, Janet; Maagerø, Eva; Tønnessen, Elise Seip

    2016-01-01

    Children as readers of picture books and the ways they respond to, and make meaning from, such texts are the focus of this article, which reports on a small-scale study undertaken in Norway and Wales, UK. The theoretical framing of the research draws on concepts of the multimodal ensemble in picture books and of the reading event as part of a…

  14. Moral Choices in Contemporary Society: A Courses by Newspaper Reader.

    Science.gov (United States)

    Rieff, Philip, Ed.; Finkle, Isaac, Ed.

    This reader, which contains 135 primary source readings about morality, is one of several college-level instructional materials developed to supplement a nationwide newspaper course on moral issues in contemporary society. The authors represent a diverse group including theologians, psychologists, politicians, professional athletes, lawyers, and…

  15. Project Physics Reader 3, The Triumph of Mechanics.

    Science.gov (United States)

    Harvard Univ., Cambridge, MA. Harvard Project Physics.

    As a supplement to Project Physics Unit 3, a collection of articles is presented in this reader for student browsing. Four excerpts are given under the following headings: On the kinetic theory of gases, Maxwell's Demon, Introduction to Waves, and Scientific Cranks. Five articles are included in terms of energy, barometers, randomness, fiddle…

  16. A Reader in International Corporate Finance, Volume Two

    OpenAIRE

    Claessens, Stijn; Laeven, Luc

    2006-01-01

    The Reader in International Corporate Finance offers an overview of current thinking on six topics: law and finance, corporate governance, banking, capital markets, capital structure and financing constraints, and the political economy of finance. This collection of 23 of the most influential articles published in the period 2000-2006 reflects two new trends: 1) interest in international ...

  17. Engaging Reluctant Readers in a French Immersion Classroom

    Science.gov (United States)

    Capina, Amanda Borton; Bryan, Gregory

    2017-01-01

    Reading engagement is like a puzzle consisting of many pieces to emplace for successful engagement to occur. The author, a French Immersion teacher/researcher, found that many of her grade one reluctant readers--those students who could read but chose not to--approached reading with some pieces of the puzzle. They had strategies and knowledge but…

  18. En guide til gyset. Horror: The Film Reader

    DEFF Research Database (Denmark)

    Toft-Nielsen, Claus

    2004-01-01

    Der er gennem tiden skrevet og teoretiseret vidt og bredt om, hvad horrorgenren er for en størrelse – og det med skiftende held. Antologien Horror: The Film Reader forsøger at levere en både bred og dybdegående introduktion til det mudrede genrefelt, som horror er. Det lykkedes langt hen af vejen....

  19. Video Vortex reader II: moving images beyond YouTube

    NARCIS (Netherlands)

    Lovink, G.; Somers Miles, R.

    2011-01-01

    Video Vortex Reader II is the Institute of Network Cultures' second collection of texts that critically explore the rapidly changing landscape of online video and its use. With the success of YouTube ('2 billion views per day') and the rise of other online video sharing platforms, the moving image

  20. ReaderBench: An Integrated Cohesion-Centered Framework

    NARCIS (Netherlands)

    Dascalu, Mihai; Stavarache, Larise; Dessus, Philippe; Trausan-Matu, Stefan; McNamara, Danielle; Bianco, Maryse

    2015-01-01

    Dascalu, M., Stavarache, L.L., Dessus, P., Trausan-Matu, S., McNamara, D.S., & Bianco, M. (2015). ReaderBench: An Integrated Cohesion-Centered Framework. In G. Conole, T. Klobucar, C. Rensing, J. Konert & É. Lavoué (Eds.), 10th European Conf. on Technology Enhanced Learning (pp. 505–508). Toledo, Sp

  1. After-School Literacy Engagements with Struggling Readers

    Science.gov (United States)

    Piazza, Susan V.; Duncan, Lonnie E.

    2012-01-01

    Parental incarceration, poverty, urban violence, and drug use can be underlying factors of academic achievement gaps between Black urban males and their counterparts. These risk factors have the potential to position low-income urban students as struggling readers. Two qualitative case studies obtained from a larger mixed methods study illustrate…

  2. Kindling: The Amazon e-Reader as an Educational Tool

    Science.gov (United States)

    Brezicki, Colin

    2011-01-01

    The revolutionary electronic reading device, Amazon's Kindle, is already obsolete. Such is the breakneck speed of technology that the machine touted to spell the death of printed books is already heading for the scrap heap, replaced by e-readers like the iPad that access the Internet, make phone calls, download movies, and connect users with all…

  3. Dose reader of dosimetric foil; Czytnik dawki folii dozymetrycznej

    Energy Technology Data Exchange (ETDEWEB)

    Machaj, B.; Strzalkowski, J.; Smolko, K.

    1997-12-31

    Read out the absorbance of a dosimetric foil is accomplished by two beam spectrophotometer. Such a solution makes possible the compensation of light source instabilities and ensures higher stability of the dose reader. The error of absorbance measurement caused by the instabilities does not exceed 0.0004 A. (author). 3 refs, 3 figs.

  4. Reader for Advanced Spoken Tamil, Parts 1 and 2.

    Science.gov (United States)

    Schiffman, Harold F.

    Part 1 of this reader consists of transcriptions of five Tamil radio plays, with exercises, notes, and discussion. Part 2 is a synopsis grammar and a glossary. Both are intended for advanced students of Tamil who have had at least two years of instruction in the spoken language at the college level. The materials have been tested in classroom use…

  5. Solar Energy Education. Reader, Part II. Sun story. [Includes glossary

    Energy Technology Data Exchange (ETDEWEB)

    1981-05-01

    Magazine articles which focus on the subject of solar energy are presented. The booklet prepared is the second of a four part series of the Solar Energy Reader. Excerpts from the magazines include the history of solar energy, mythology and tales, and selected poetry on the sun. A glossary of energy related terms is included. (BCS)

  6. Big Books from Little Voices: Reaching High Risk Beginning Readers.

    Science.gov (United States)

    Trachtenburg, Phyllis; Ferruggia, Ann

    1989-01-01

    Discusses how interactive, whole class techniques (using a student-generated Big Book adaptation of "Corduroy") improved the reading skills of high risk first grade readers. Describes several activities, including sight word strategies, decoding techniques, and word processing, and suggests 27 Big Books for use with these activities. (MM)

  7. Using Explicit Instruction to Promote Vocabulary Learning for Struggling Readers

    Science.gov (United States)

    Taylor, D. Bruce; Mraz, Maryann; Nichols, William D.; Rickelman, Robert J.; Wood, Karen D.

    2009-01-01

    Research supports the need for active vocabulary learning across grade levels and subject areas to help increase readers' comprehension of diverse texts that they encounter. Given the increasing emphasis on decoding and reading comprehension, the relative importance of vocabulary instruction has been diminished in recent years. The authors argue…

  8. Information Utility, Reader Interest, Publication Rating and Student Newspaper Readership.

    Science.gov (United States)

    Lin, Carolyn A.

    2000-01-01

    Investigates student motivation for reading a college student newspaper, including evaluations of performance and content preferences. Finds general support for expectations derived from utility theory; that localism (i.e. campus news) remains one of the strongest niches for an urban college paper; and that heavier readers perceived higher utility…

  9. The Embedded and Embodied Literacies of a Young Reader

    Science.gov (United States)

    Mackey, Margaret

    2011-01-01

    This article argues that if we paid attention to the local situation of a reader the way we attend to the life story of an author, we might gain a very different understanding of children's literacy. It explores the literate approaches of a single child exploring a single theme--the settler culture as represented in a variety of materials…

  10. Gamers are readers: capitalize on the popularity of video games

    National Research Council Canada - National Science Library

    Easterwood, Lori; Wesson, Lindsey Patrick

    2009-01-01

    ... programs that attract so many teens. However, librarians are failing to capitalize on the popularity of video games by using the most fundamental of all librarian skills: reader's advisory. If a gamer came to you 30 years ago raving about Pong, you may have been hard pressed to find a corollary in literature. Today's video games, on the other hand, are ...

  11. Medical Readers' Theater: Relevance to Geriatrics Medical Education

    Science.gov (United States)

    Shapiro, Johanna; Cho, Beverly

    2011-01-01

    Medical Readers' Theater (MRT) is an innovative and simple way of helping medical students to reflect on difficult-to-discuss topics in geriatrics medical education, such as aging stereotypes, disability and loss of independence, sexuality, assisted living, relationships with adult children, and end-of-life issues. The authors describe a required…

  12. MEANTIME, the NewsReader Multilingual Event and Time Corpus

    NARCIS (Netherlands)

    Minard, A.-L.; Speranza, M; Urizar, R.; Altuna, B.; Erp, van M.G.J.; Schoen, A.M.; Son, van C.M.

    2016-01-01

    In this paper, we present the NewsReader MEANTIME corpus, a semantically annotated corpus of Wikinews articles. The corpus consists of 480 news articles, i.e. 120 English news articles and their translations in Spanish, Italian, and Dutch. MEANTIME contains annotations at different levels. The docum

  13. What It Means to Be Strategic: Good Readers as Informants.

    Science.gov (United States)

    Harste, Jerome C.

    A study was conducted to identify strategies used by successful readers in comprehending and interpreting various kinds of texts. Seventy-three graduate students were asked to keep a journal (unedited and freely written) of what they were thinking as they were reading Umberto Eco's novel "The Name of the Rose." Selected journal entries…

  14. Sloth, Silence, and Concentration: The Reader between Letter and Spirit

    Science.gov (United States)

    Meijer, Wilna A. J.

    2009-01-01

    In current-day criticisms of consumer culture as well as of the performativity culture of the work place, the originally religious concept of the vice of acedia or sloth is reinterpreted as a virtue. Art, especially poetry, is put to the fore in that connection. The image of the reader is addressed when the question of the relationship between…

  15. Constructing Proximity: Relating to Readers in Popular and Professional Science

    Science.gov (United States)

    Hyland, Ken

    2010-01-01

    The view of academic discourse as a rhetorical activity involving interactions between writers and readers is now central to most perspectives on EAP, but these interactions are conducted differently in different disciplinary and generic contexts. In this paper I use the term "proximity" to refer to a writer's control of those rhetorical features…

  16. Video Vortex reader II: moving images beyond YouTube

    NARCIS (Netherlands)

    Lovink, G.; Somers Miles, R.

    2011-01-01

    Video Vortex Reader II is the Institute of Network Cultures' second collection of texts that critically explore the rapidly changing landscape of online video and its use. With the success of YouTube ('2 billion views per day') and the rise of other online video sharing platforms, the moving image h

  17. Reader Response Journals: Novice Teachers Reflect on Their Implementation Process

    Science.gov (United States)

    McIntosh, Janet E.

    2010-01-01

    Do novice teachers effectively use teaching strategies they learn in a preservice course? This study examines how five novice intermediate (Grades 7-10) English language arts teachers implemented reader response journals, an instructional activity they learned about in their preservice English methods course. During interviews, they shared the…

  18. Utilizing Psycholinguistic Insights in Teaching via the Basal Reader.

    Science.gov (United States)

    Newman, Harold

    Ideas of educational psycholinguists Frank Smith and Kenneth Goodman can be combined with the ideas presented in current basal reader manuals to help teachers teach reading more effectively. Since reading and speaking are parallel processes, teachers may invite children to "read" with them, hearing the melody of language as they point to…

  19. Students Attendance Management System Based On RFID And Fingerprint Reader

    Directory of Open Access Journals (Sweden)

    Moth Moth Myint Thein

    2015-07-01

    Full Text Available Abstract Today students class attendance is become more important part for any organizationsinstitutions. The conventional method of taking attendance by calling names or signing on paper is very time consuming and insecure hence inefficient. This paper presents the manual students attendance management into computerized system for convenience or data reliability. So the system is developed by the integration of ubiquitous computing systems into classroom for managing the students attendance using RFID and fingerprint reader. The system is designed to implement an attendance management system based on RFID and fingerprint reader which students need to use their student identification card ID and their finger ID to success the attendance where only authentic student can be recorded the attendance during the class. In this system passive RFID tag and reader pairs are used to register the student ID cards individually and fingerprint reader is used for attendance. This system takes attendance electronically with the help of the RFID and finger print device and the records of the attendance are stored in a database. Students roll call percentages and their details are easily seenvia Graphical User Interface GUI. This system will have the required databases for students attendance teachers subjects and students details. This application is implemented by Microsoft Visual Studio and Microsoft SQL Server as IDE. C language is used to implement this system.

  20. Selecting literature for beginner readers in South Africa

    Directory of Open Access Journals (Sweden)

    Marius Crous

    2012-04-01

    Full Text Available This article explores complexities of choosing appropriate reading material which may provide maximum engagement for beginner readers in Southern Africa, with the aim of instilling a lifelong love of reading. The article presents a case in favour of literary criteria as base line for choices of reading material for beginner readers. Furthermore, the article outlines general characteristics of reading material for beginner readers, as well as specific criteria to be considered in the Southern African context, taking into account the linguistic diversity which exists in formal education and the vast literacy backlog in the region. Furthermore, it is argued that translated children’s stories should ideally be adapted rather than literally translated, in order to meet the requirements of what is universally regarded as good children’s literature for beginner readers. A culture of reading can only be instilled by adhering to the following criteria, i.e. ensuring affective engagement, maximum meaning-making potential, delight, the engagement of the imagination for the purposes of developing the creative mind, and strong identification with the material for the purposes of developing a sense of agency.

  1. Suggestopedia: A Teaching Strategy for the Severely Disabled Reader.

    Science.gov (United States)

    James, Bobby M.

    The use of suggestopedia--a technique combining memory expansion and relaxation--as a teaching technique for disabled readers is examined in this paper. The paper first describes the origin of suggestopedia as a therapeutic technique in Bulgaria; it then describes some components of suggestopedia: the creation of physical relaxation, the use of…

  2. Uncontracted or Contracted Braille for Emergent Readers: A Pilot Study

    Science.gov (United States)

    Farnsworth, Charles, Jr.

    2007-01-01

    To determine the attitudes of teachers of students who are visually impaired or blind about the use of contracted or uncontracted Braille for emergent readers, the author posted a questionnaire on three electronic listservs from October through December 2002 and received responses from 40 teachers in India, Canada, the West Indies, and the United…

  3. Beyond Decoding: Phonological Processing during Silent Reading in Beginning Readers

    Science.gov (United States)

    Blythe, Hazel I.; Pagán, Ascensión; Dodd, Megan

    2015-01-01

    In this experiment, the extent to which beginning readers process phonology during lexical identification in silent sentence reading was investigated. The eye movements of children aged seven to nine years and adults were recorded as they read sentences containing either a correctly spelled target word (e.g., girl), a pseudohomophone (e.g., gerl),…

  4. The Embedded and Embodied Literacies of a Young Reader

    Science.gov (United States)

    Mackey, Margaret

    2011-01-01

    This article argues that if we paid attention to the local situation of a reader the way we attend to the life story of an author, we might gain a very different understanding of children's literacy. It explores the literate approaches of a single child exploring a single theme--the settler culture as represented in a variety of materials…

  5. Every Reader a Reviewer: The Online Book Conversation

    Science.gov (United States)

    Hoffert, Barbara

    2010-01-01

    Over the last 15 years, the book review landscape has changed seismically. Reviewing is no longer centralized, with a few big voices leading the way, but fractured among numerous multifarious voices found mostly on the web. In turn, readers aren't playing the captive audience any more. Undone by economics, many traditional print sources have been…

  6. Mi Familia (My Family). Chula Vista Readers, Book One.

    Science.gov (United States)

    Van Olst, Ofelia; And Others

    This is book one in a series of beginning readers in Spanish for preschool and grade one. The program is designed to prepare children to read in Spanish by teaching the sound-symbol relationship in sequential order with simple reading selections. This book introduces the consonants "m,""p,""s,""l," and the…

  7. An Analysis of the Learning Styles of Underachieving Readers.

    Science.gov (United States)

    Corlett, Donna

    1992-01-01

    Finds no significant learning style elements that were different between genders in at-risk, underachieving readers. Advocates offering at-risk students a rich choice of learning opportunities so that they can choose the learning/teaching styles that best suit them. (SR)

  8. Reading and Spelling in Good and Poor Readers

    Science.gov (United States)

    Camp, Bonnie W.; Dolcourt, Jack L.

    1977-01-01

    To more easily distinguish patterns of reading and spelling errors, two parallel, standardized reading and spelling forms were designed (based on E. Boder's work) and used to test two groups of children: 34 children from regular fifth-grade classes, and 18 fourth- to sixth-grade children diagnosed as retarded readers. (Author)

  9. The Fluid Reading Primer: Animated Decoding Support for Emergent Readers.

    Science.gov (United States)

    Zellweger, Polle T.; Mackinlay, Jock D.

    A prototype application called the Fluid Reading Primer was developed to help emergent readers with the process of decoding written words into their spoken forms. The Fluid Reading Primer is part of a larger research project called Fluid Documents, which is exploring the use of interactive animation of typography to show additional information in…

  10. Sea Songs: Readers Theatre from the South Pacific

    Science.gov (United States)

    Barnes, James

    2004-01-01

    Allow students to experience the richness of Polynesian culture as well as the challenge of dramatic reading with this Readers Theatre resource. During his years of living and teaching in Oamaru, New Zealand, James Barnes became intimately involved in the Maori culture. Through extensive research of the mythology of Polynesia, Barnes succeeded in…

  11. En guide til gyset. Horror: The Film Reader

    DEFF Research Database (Denmark)

    Toft-Nielsen, Claus

    2004-01-01

    Der er gennem tiden skrevet og teoretiseret vidt og bredt om, hvad horrorgenren er for en størrelse – og det med skiftende held. Antologien Horror: The Film Reader forsøger at levere en både bred og dybdegående introduktion til det mudrede genrefelt, som horror er. Det lykkedes langt hen af vejen....

  12. Intermittent plate tectonics?

    Science.gov (United States)

    Silver, Paul G; Behn, Mark D

    2008-01-04

    Although it is commonly assumed that subduction has operated continuously on Earth without interruption, subduction zones are routinely terminated by ocean closure and supercontinent assembly. Under certain circumstances, this could lead to a dramatic loss of subduction, globally. Closure of a Pacific-type basin, for example, would eliminate most subduction, unless this loss were compensated for by comparable subduction initiation elsewhere. Given the evidence for Pacific-type closure in Earth's past, the absence of a direct mechanism for termination/initiation compensation, and recent data supporting a minimum in subduction flux in the Mesoproterozoic, we hypothesize that dramatic reductions or temporary cessations of subduction have occurred in Earth's history. Such deviations in the continuity of plate tectonics have important consequences for Earth's thermal and continental evolution.

  13. VISAS AND GREEN PLATES

    CERN Multimedia

    2000-01-01

    From 3 April 2000, all questions relating to visa requests for Switzerland, France, or Russia for a member of the personnel must be addressed to Ms. Agnita Querrou (telephone 72838, office 5-2-019, e-mail Agnita.Querrou@cern.ch).The Users' Office continues to deal with requests for letters of invitation and questions concerning visas for users in EP Division.Questions relating to removals, requests for green plates, to privileges of members of the personnel and to the importation of vehicles are still dealt with by Ms Zuzana Miller (telephone 79257, office 33-1-017, e-mail Zuzana.Muller@cern.ch) and Ms Joëlle Belleman (telephone 73962, office 33-1-019, e-mail Joelle.Belleman@cern.ch).

  14. Fluorescence Polarization Assays in Small Molecule Screening

    Science.gov (United States)

    Lea, Wendy A.; Simeonov, Anton

    2011-01-01

    Importance of the field Fluorescence polarization (FP) is a homogeneous method that allows rapid and quantitative analysis of diverse molecular interactions and enzyme activities. This technique has been widely utilized in clinical and biomedical settings, including the diagnosis of certain diseases and monitoring therapeutic drug levels in body fluids. Recent developments in the field has been symbolized by the facile adoption of FP in high-throughput screening (HTS) and small molecule drug discovery of an increasing range of target classes. Areas covered in this review The article provides a brief overview on the theoretical foundation of FP, followed by updates on recent advancements in its application for various drug target classes, including G-protein coupled receptors (GPCRs), enzymes and protein-protein interactions (PPIs). The strengths and weaknesses of this method, practical considerations in assay design, novel applications, and future directions are also discussed. What the reader will gain The reader will be informed of the most recent advancements and future directions of FP application to small molecule screening. Take home message In addition to its continued utilization in high-throughput screening, FP has expanded into new disease and target areas and has been marked by increased use of labeled small molecule ligands for receptor binding studies. PMID:22328899

  15. Plate osteosynthesis of simple forearm fractures : LCP versus DC plates

    NARCIS (Netherlands)

    Stevens, Charles Tjerk; Ten Duis, Henk Jan

    2008-01-01

    The aim of this study was to compare the time to radiological bony union of simple A-type fractures of the forearm, treated with either a locking compression plate (LCP) or a dynamic compression plate (DCP). For each fracture, the relation between the use of compression and radiological healing time

  16. Plate osteosynthesis of simple forearm fractures : LCP versus DC plates

    NARCIS (Netherlands)

    Stevens, Charles Tjerk; Ten Duis, Henk Jan

    The aim of this study was to compare the time to radiological bony union of simple A-type fractures of the forearm, treated with either a locking compression plate (LCP) or a dynamic compression plate (DCP). For each fracture, the relation between the use of compression and radiological healing time

  17. Inter-reader variability in alternate forced choice studies

    Science.gov (United States)

    Huda, W.; Ogden, K. M.; Samei, E.; Scalzetti, E. M.; Lavallee, R. L.; Roskopf, M. L.

    2008-03-01

    In this study, we investigated differences in detection performance for twelve observers who each generated a CT contrast detail curve. An anthropomorphic newborn phantom's abdomen was imaged using a GE Light Speed CT scanner (4-slice). Alternate Forced Choice (AFC) experiments were performed with lesions sizes ranging from 2.5 to 12.5 mm to determine the intensity needed to achieve 92% correct (I 92%). Following training, twelve readers consisting of (2 technologists, 4 college students, 4 medical students, and 2 radiology residents) generated a single contrast detail curve. Eight readers produced approximately linear contrast detail curves while the remaining four readers required a second order polynomial fit because of reduced performance when detecting the largest (i.e., 12.5 mm) lesion. For the three smallest lesions, the coefficient of variation between the twelve readers was ~12%, which increases with increasing lesion size to ~23% for 12.5 mm lesion size. The ratio of the maximum I 92% to minimum I 92% values was ~1.6 for the smallest lesions, which increased to a factor of ~2.1 for the 12.5 mm lesion. Our results show that minimizing inter-reader variability in our AFC experiments could be achieved by eliminating the largest lesion that cause detection problems in one third of observers. The combined experimental data showed that the slope of the contrast detail curve was -0.42, lower than the value of -1.0 predicted by the Rose model, suggesting that the noise texture in CT associated with both quantum mottle and anatomic structure is an important factor affecting detection of these lesions.

  18. Plate tectonics, damage and inheritance.

    Science.gov (United States)

    Bercovici, David; Ricard, Yanick

    2014-04-24

    The initiation of plate tectonics on Earth is a critical event in our planet's history. The time lag between the first proto-subduction (about 4 billion years ago) and global tectonics (approximately 3 billion years ago) suggests that plates and plate boundaries became widespread over a period of 1 billion years. The reason for this time lag is unknown but fundamental to understanding the origin of plate tectonics. Here we suggest that when sufficient lithospheric damage (which promotes shear localization and long-lived weak zones) combines with transient mantle flow and migrating proto-subduction, it leads to the accumulation of weak plate boundaries and eventually to fully formed tectonic plates driven by subduction alone. We simulate this process using a grain evolution and damage mechanism with a composite rheology (which is compatible with field and laboratory observations of polycrystalline rocks), coupled to an idealized model of pressure-driven lithospheric flow in which a low-pressure zone is equivalent to the suction of convective downwellings. In the simplest case, for Earth-like conditions, a few successive rotations of the driving pressure field yield relic damaged weak zones that are inherited by the lithospheric flow to form a nearly perfect plate, with passive spreading and strike-slip margins that persist and localize further, even though flow is driven only by subduction. But for hotter surface conditions, such as those on Venus, accumulation and inheritance of damage is negligible; hence only subduction zones survive and plate tectonics does not spread, which corresponds to observations. After plates have developed, continued changes in driving forces, combined with inherited damage and weak zones, promote increased tectonic complexity, such as oblique subduction, strike-slip boundaries that are subparallel to plate motion, and spalling of minor plates.

  19. Low-Skilled Adult Readers Look Like Typically Developing Child Readers: A Comparison of Reading Skills and Eye Movement Behavior

    Science.gov (United States)

    Barnes, Adrienne E.; Kim, Young-Suk

    2016-01-01

    Adults enrolled in basic education exhibit poor academic performance, often reading at elementary and middle-school levels. The current study investigated the similarities and differences of reading skills and eye movement behavior between a sample of 25 low-skilled adult readers and 25 first grade students matched on word reading skill. t tests…

  20. Mind the Gap: Bridging the Divide between Non-Readers and Lifelong Readers with Hi-Lo Books

    Science.gov (United States)

    Gleason, Carrie

    2011-01-01

    In the past few years, there has been increasing demand from school librarians for books for "reluctant readers"--despite the impressive offering of children's literature published each year and the success of blockbuster series like "Harry Potter, Diary of a Wimpy Kid, Twilight," and "Hunger Games," among others. This is also in addition to all…

  1. Mind the Gap: Bridging the Divide between Non-Readers and Lifelong Readers with Hi-Lo Books

    Science.gov (United States)

    Gleason, Carrie

    2011-01-01

    In the past few years, there has been increasing demand from school librarians for books for "reluctant readers"--despite the impressive offering of children's literature published each year and the success of blockbuster series like "Harry Potter, Diary of a Wimpy Kid, Twilight," and "Hunger Games," among others. This is also in addition to all…

  2. Brazed aluminum, Plate-fin heat exchangers for OTEC

    Energy Technology Data Exchange (ETDEWEB)

    Foust, H.D.

    1980-12-01

    Brazed aluminum plate-fin heat exchangers have been available for special applications for over thirty years. The performance, compactness, versatility, and low cost of these heat exchangers has been unequaled by other heat exchanger configuration. The application of brazed aluminum has been highly limited because of necessary restrictions for clean non-corrosive atmospheres. Air and gas separation have provided ideal conditions for accepting brazed aluminum and in turn have benefited by the salient features of these plate-fin heat exchangers. In fact, brazed aluminum and cryogenic gas and air separation have become nearly synonymous. Brazed aluminum in its historic form could not be considered for a seawater atmosphere. However, technology presents a new look of significant importance to OTEC in terms of compactness and cost. The significant technological variation made was to include one-piece hollow extensions for the seawater passages. Crevice corrosion sites are thereby entirely eliminated and pitting corrosion attack will be controlled by an integral and sacrificial layer of a zinc-aluminum alloy. This paper on brazed aluminum plate-fin heat exchangers for OTEC will aquaint the reader with the state-of-art and variations suggested to qualify this form of aluminum for seawater use. In order to verify the desirable cost potential for OTEC, Trane teamed with Westinghouse to perform an OTEC system analysis with this heat exchanger. These results are very promising and reported in detail elsewhere.

  3. Casimir Effect for Dielectric Plates

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    We generalize Kupisewska method to the three-dimensional system and another derivation of the Casimir effect between two dielectric plates is presented based on the explicit quantization of the electromagnetic field in the presence of dielectrics, where the physical meaning of "evanescent mode" is discussed. The Lifshitz's formula is rederived perfect metallic plates will the evanescent modes become unimportant.

  4. Micro-channel plate detector

    Science.gov (United States)

    Elam, Jeffrey W.; Lee, Seon W.; Wang, Hsien -Hau; Pellin, Michael J.; Byrum, Karen; Frisch, Henry J.

    2015-09-22

    A method and system for providing a micro-channel plate detector. An anodized aluminum oxide membrane is provided and includes a plurality of nanopores which have an Al coating and a thin layer of an emissive oxide material responsive to incident radiation, thereby providing a plurality of radiation sensitive channels for the micro-channel plate detector.

  5. Gold plating on spectacle frames.

    Science.gov (United States)

    Kenny, I; Mitchell, J W; Walsh, G

    1997-05-01

    An investigation was carried out into the thickness and standard of application of the plating and lacquer coatings applied to three metal spectacle frames. All conform to BS 6625 (1991) for plating thickness, but there was considerable variation in regularity and porosity.

  6. Fluorescence antibunching microscopy

    CERN Document Server

    Schwartz, Osip

    2011-01-01

    Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of fluorescent markers, routinely used as contrast labels for bio-imaging. The technique can be readily implemented using standard fluorescence microscopy equipment.

  7. Fluorescence of atopic allergens

    NARCIS (Netherlands)

    Berrens, L.

    1967-01-01

    Purified atopic allergens have been found to emit flue fluorescence upon irradiation with ultraviolet light of 365 mμ wavelength. The maximum of fluorescence is in the region 445–490 mμ and the intensity is of the same order of magnitude for different atopic allergens. Synthetic model compounds, inc

  8. Fluorescent Lamp Replacement Study

    Science.gov (United States)

    2017-07-01

    Light-emitting diode, LED, lighting, fluorescent, waste reduction, energy conservation, net zero , mercury 16. SECURITY CLASSIFICATION OF: 17. LIMITATION...Center (ATC) to assess the benefits of converting fluorescent tube lighting to light-emitting diode (LED) technology. The report documents the waste ...1-15 SECTION 2. SUBTESTS 2.1 HAZARDOUS WASTE REDUCTION

  9. LEDs for fluorescence microscopy

    NARCIS (Netherlands)

    Young, I.T.; Garini, Y.; Dietrich, H.R.C.; Van Oel, W.; Liqui Lung, G.

    2004-01-01

    Traditional light sources for fluorescence microscopy have been mercury lamps, xenon lamps, and lasers. These sources have been essential in the development of fluorescence microscopy but each can have serious disadvantages: lack of near monochromaticity, heat generation, cost, lifetime of the light

  10. Plate shell structures of glass

    DEFF Research Database (Denmark)

    Bagger, Anne

    . This modelling technique is used to model a plate shell structure with a span of 11.5 meters in the FE software \\textsc{Abaqus}. The structure is analyzed with six different connection details with varying stiffness characteristics, to investigate the influence of these characteristics on the structural effects...... University, a script has been developed for an automated generation of a given plate shell geometry and a corresponding finite element (FE) model. A suitable FE modelling technique is proposed, suggesting a relatively simple method of modelling the connection detail's stiffness characteristics....... Based on these investigations, and FE analysis of other plate shell models, the structural behaviour is described. Possible methods of estimating the stresses in a given plate shell structure are proposed. The non-linear behaviour of a plate shell structure is investigated for varying parameters...

  11. The moving plate capacitor paradox

    Science.gov (United States)

    Davis, B. R.; Abbott, D.; Parrondo, J. M. R.

    2000-03-01

    For the first time we describe an apparent paradox concerning a moving plate capacitor driven by thermal noise from a resistor. A demon restores the plates of the capacitor to their original position, only when the voltage across the capacitor is small—hence only small forces are present for the demon to work against. The demon has to work harder than this to avoid the situation of perpetual motion, but the question is how? We explore the concept of a moving plate capacitor, driven by noise, a step further by examining the case where the restoring force on the capacitor plates is provided by a simple spring, rather than some unknown demon. We display simulation results with interesting behavior, particularly where the capacitor plates collide with each other.

  12. SAMI Automated Plug Plate Configuration

    CERN Document Server

    Lorente, Nuria P F; Goodwin, Michael

    2012-01-01

    The Sydney-AAO Multi-object Integral field spectrograph (SAMI) is a prototype wide-field system at the Anglo-Australian Telescope (AAT) which uses a plug-plate to mount its 13 x 61-core imaging fibre bundles (hexabundles) in the optical path at the telescope's prime focus. In this paper we describe the process of determining the positions of the plug-plate holes, where plates contain three or more stacked observation configurations. The process, which up until now has involved several separate processes and has required significant manual configuration and checking, is now being automated to increase efficiency and reduce error. This is carried out by means of a thin Java controller layer which drives the configuration cycle. This layer controls the user interface and the C++ algorithm layer where the plate configuration and optimisation is carried out. Additionally, through the Aladin display package, it provides visualisation and facilitates user verification of the resulting plates.

  13. Thin plate neotectonic models of the Australian plate

    Science.gov (United States)

    Burbidge, D. R.

    2004-10-01

    Thin plate finite element models of the neotectonic deformation of the Australian plate have been calculated in order to estimate the stress and strain rate within the plate, specifically concentrating on the Australian continent. The model includes plate-bounding faults, an anelastic brittle-ductile layered rheology and the option of laterally varying elevation and heat flow. The results of the models are compared to (1) the velocity of geodetic benchmarks on the Australian plate, (2) the spreading rate of the mid-oceanic ridges along the Australian plate's margins, (3) the direction of the maximum horizontal principal stress, (4) the stress regime within the plate, and (5) the crustal thickness estimated from the depth to the base of Mohorovicic discontinuity's transition zone. A variety of models are tested with a wide range of input parameters. The model with the smallest misfit with observations predicts that the strain rate for most of the Australian continent is approximately 10-17 s-1. This model has a slightly lower strain rate in the central Australia and is higher off the northern coast of Australia than for the rest of the continent. Strain rates of this magnitude would be difficult to observe from geodetic or geologic data for most parts of Australia but would be enough to generate much of the seismicity that has been observed over the last century.

  14. Underwater electrical discharge in plate to plate configuration

    Science.gov (United States)

    Stelmashuk, Vitaliy

    2016-09-01

    Two main configurations of high voltage electrodes submersed in water have been used for an electrical discharge generation: pin to pin and pin to plate. An electrical breakdown between plate electrodes is generally difficult to reproduce, because there is a uniform and weak electric field. One major advantage of using plate electrodes is their greater ``wear hardness'' to high-energy discharges. The plate electrodes can withstand extremely high energy deposition at which the pin electrode is quickly destroyed. The electrical discharge between plate electrodes can be initiated by creating an inhomogeneity in the electrical field. Two methods of discharge initiation between plate electrodes are proposed for this aim: 1) focusing of a shock wave in the interelectrode region; 2) a bubble injection into the electrode gap. The shock wave creates favourable conditions for the electrical breakdown between the two plate electrodes: it causes that numerous microbubbles of dissolved air start to grow and serve as locations for streamer initiation. In the second method the gas bubble is injected from the one of the electrodes, which has a gas inlet hole on the lateral face for this purpose. A ``volcano'' like morphology of positive streamers are observed in the experiments with weak electric field. The authors are grateful to MEYS grant INGO LG 15013.

  15. Highly curved microchannel plates

    Science.gov (United States)

    Siegmund, O. H. W.; Cully, S.; Warren, J.; Gaines, G. A.; Priedhorsky, W.; Bloch, J.

    1990-01-01

    Several spherically curved microchannel plate (MCP) stack configurations were studied as part of an ongoing astrophysical detector development program, and as part of the development of the ALEXIS satellite payload. MCP pairs with surface radii of curvature as small as 7 cm, and diameters up to 46 mm have been evaluated. The experiments show that the gain (greater than 1.5 x 10 exp 7) and background characteristics (about 0.5 events/sq cm per sec) of highly curved MCP stacks are in general equivalent to the performance achieved with flat MCP stacks of similar configuration. However, gain variations across the curved MCP's due to variations in the channel length to diameter ratio are observed. The overall pulse height distribution of a highly curved surface MCP stack (greater than 50 percent FWHM) is thus broader than its flat counterpart (less than 30 percent). Preconditioning of curved MCP stacks gives comparable results to flat MCP stacks, but it also decreases the overall gain variations. Flat fields of curved MCP stacks have the same general characteristics as flat MCP stacks.

  16. Indonesian Landforms and Plate Tectonics

    Directory of Open Access Journals (Sweden)

    Herman Th. Verstappen

    2014-06-01

    Full Text Available DOI: 10.17014/ijog.v5i3.103The horizontal configuration and vertical dimension of the landforms occurring in the tectonically unstable parts of Indonesia were resulted in the first place from plate tectonics. Most of them date from the Quaternary and endogenous forces are ongoing. Three major plates – the northward moving Indo-Australian Plate, the south-eastward moving SE-Asian Plate and the westward moving Pacific Plate - meet at a plate triple-junction situated in the south of New Guinea’s Bird’s Head. The narrow North-Moluccan plate is interposed between the Asia and Pacific. It tapers out northward in the Philippine Mobile Belt and is gradually disappearing. The greatest relief amplitudes occur near the plate boundaries: deep ocean trenches are associated with subduction zones and mountain ranges with collision belts. The landforms of the more stable areas of the plates date back to a more remote past and, where emerged, have a more subdued relief that is in the first place related to the resistance of the rocks to humid tropical weathering Rising mountain ranges and emerging island arcs are subjected to rapid humid-tropical river erosions and mass movements. The erosion products accumulate in adjacent sedimentary basins where their increasing weight causes subsidence by gravity and isostatic compensations. Living and raised coral reefs, volcanoes, and fault scarps are important geomorphic indicators of active plate tectonics. Compartmental faults may strongly affect island arcs stretching perpendicular to the plate movement. This is the case on Java. Transcurrent faults and related pull-apart basins are a leading factor where plates meet at an angle, such as on Sumatra. The most complicated situation exists near the triple-junction and in the Moluccas. Modern research methods, such as GPS measurements of plate movements and absolute dating of volcanic outbursts and raised coral reefs are important tools. The mega-landforms resulting

  17. Indonesian Landforms and Plate Tectonics

    Directory of Open Access Journals (Sweden)

    Herman Th. Verstappen

    2014-06-01

    Full Text Available DOI: 10.17014/ijog.v5i3.103The horizontal configuration and vertical dimension of the landforms occurring in the tectonically unstable parts of Indonesia were resulted in the first place from plate tectonics. Most of them date from the Quaternary and endogenous forces are ongoing. Three major plates – the northward moving Indo-Australian Plate, the south-eastward moving SE-Asian Plate and the westward moving Pacific Plate - meet at a plate triple-junction situated in the south of New Guinea’s Bird’s Head. The narrow North-Moluccan plate is interposed between the Asia and Pacific. It tapers out northward in the Philippine Mobile Belt and is gradually disappearing. The greatest relief amplitudes occur near the plate boundaries: deep ocean trenches are associated with subduction zones and mountain ranges with collision belts. The landforms of the more stable areas of the plates date back to a more remote past and, where emerged, have a more subdued relief that is in the first place related to the resistance of the rocks to humid tropical weathering Rising mountain ranges and emerging island arcs are subjected to rapid humid-tropical river erosions and mass movements. The erosion products accumulate in adjacent sedimentary basins where their increasing weight causes subsidence by gravity and isostatic compensations. Living and raised coral reefs, volcanoes, and fault scarps are important geomorphic indicators of active plate tectonics. Compartmental faults may strongly affect island arcs stretching perpendicular to the plate movement. This is the case on Java. Transcurrent faults and related pull-apart basins are a leading factor where plates meet at an angle, such as on Sumatra. The most complicated situation exists near the triple-junction and in the Moluccas. Modern research methods, such as GPS measurements of plate movements and absolute dating of volcanic outbursts and raised coral reefs are important tools. The mega-landforms resulting

  18. Reader Response: An Alternative Way to Teach Students to Think about Text.

    Science.gov (United States)

    Chase, Nancy D.; Hynd, Cynthia R.

    1987-01-01

    Describes the fundamentals of reader response theory, focuses on the aspects most relevant to reading instruction, and presents a method of teaching using reader response as a vehicle for improving student ability to learn from text. (NKA)

  19. Alternate Reading Strategies and Variable Asymmetry of the Planum Temporale in Adult Resilient Readers

    Science.gov (United States)

    Welcome, Suzanne E.; Leonard, Christiana M.; Chiarello, Christine

    2010-01-01

    Resilient readers are characterized by impaired phonological processing despite skilled text comprehension. We investigated orthographic and semantic processing in resilient readers to examine mechanisms of compensation for poor phonological decoding. Performance on phonological (phoneme deletion, pseudoword reading), orthographic (orthographic…

  20. Orthographic and phonological processing in developing readers revealed by ERPs.

    Science.gov (United States)

    Eddy, Marianna D; Grainger, Jonathan; Holcomb, Phillip J; Gabrieli, John D E

    2016-12-01

    The development of neurocognitive mechanisms in single word reading was studied in children ages 8-10 years using ERPs combined with priming manipulations aimed at dissociating orthographic and phonological processes. Transposed-letter (TL) priming (barin-BRAIN vs. bosin-BRAIN) was used to assess orthographic processing, and pseudohomophone (PH) priming (brane-BRAIN vs. brant-BRAIN) was used to assess phonological processing. Children showed TL and PH priming effects on both the N250 and N400 ERP components, and the magnitude of TL priming correlated positively with reading ability, with better readers showing larger TL priming effects. Phonological priming, on the other hand, did not correlate with reading ability. The positive correlations between TL priming and reading ability in children points to a key role for flexible sublexical orthographic representations in reading development, in line with their hypothesized role in the efficient mapping of orthographic information onto semantic information in skilled readers. © 2016 Society for Psychophysiological Research.

  1. Optimised to Fail: Card Readers for Online Banking

    Science.gov (United States)

    Drimer, Saar; Murdoch, Steven J.; Anderson, Ross

    The Chip Authentication Programme (CAP) has been introduced by banks in Europe to deal with the soaring losses due to online banking fraud. A handheld reader is used together with the customer’s debit card to generate one-time codes for both login and transaction authentication. The CAP protocol is not public, and was rolled out without any public scrutiny. We reverse engineered the UK variant of card readers and smart cards and here provide the first public description of the protocol. We found numerous weaknesses that are due to design errors such as reusing authentication tokens, overloading data semantics, and failing to ensure freshness of responses. The overall strategic error was excessive optimisation. There are also policy implications. The move from signature to PIN for authorising point-of-sale transactions shifted liability from banks to customers; CAP introduces the same problem for online banking. It may also expose customers to physical harm.

  2. Exploring Ghost Worlds: A Review of 'The Daniel Clowes Reader'

    Directory of Open Access Journals (Sweden)

    Paddy Johnston

    2013-09-01

    Full Text Available Daniel Clowes is undoubtedly one of the most influential and prolific cartoonists working today, with a career spanning many decades. 'The Daniel Clowes Reader' (Parille 2013 comes at the perfect time – when interest in Clowes from scholars and critics is at a high, but in which he is still perhaps given less critical attention than his peers Chris Ware, Art Spiegelman, and Marjane Satrapi, all of whom are cited twice as often as Clowes despite his large canon of significant works in comics. With recent books being published on Chris Ware by The University Press of Mississippi and the forthcoming Art Spiegelman collection from Drawn and Quarterly, more significant focused, monographic books are emerging in comics and comics criticism, and 'The Daniel Clowes Reader' is a more than welcome addition to this emergence.

  3. Inner speech during silent reading reflects the reader's regional accent.

    Directory of Open Access Journals (Sweden)

    Ruth Filik

    Full Text Available While reading silently, we often have the subjective experience of inner speech. However, there is currently little evidence regarding whether this inner voice resembles our own voice while we are speaking out loud. To investigate this issue, we compared reading behaviour of Northern and Southern English participants who have differing pronunciations for words like 'glass', in which the vowel duration is short in a Northern accent and long in a Southern accent. Participants' eye movements were monitored while they silently read limericks in which the end words of the first two lines (e.g., glass/class would be pronounced differently by Northern and Southern participants. The final word of the limerick (e.g., mass/sparse then either did or did not rhyme, depending on the reader's accent. Results showed disruption to eye movement behaviour when the final word did not rhyme, determined by the reader's accent, suggesting that inner speech resembles our own voice.

  4. Social Imaginary in web advertising of e-readers

    Directory of Open Access Journals (Sweden)

    Silvina Marcela Angelozzi

    2015-04-01

    Full Text Available This paper explores the imaginary meanings that appear in the advertising discourses on e-book reading devices commonly known as e-readers. It starts from the assumption that imaginary meanings are associated with both: the imaginary associated to digital technologies (Cabrera, 2006, 2011 and to the imaginary of "order of books" (Chartier, 2000, 2005. In order to carry out the exploration, the advertising discourses in the homepages of three cases are analized: The Amazon Kindle, Kobo and MovistarBq brands. The conclusion is that e-readers are devices that participate of the imaginary of the digital technologies as other devices in the complex of digital technologies in everyday life, and also refers strongly to the imaginary meanings related to printed books.

  5. Fluorescence and Spectral Imaging

    Directory of Open Access Journals (Sweden)

    Ralph S. DaCosta

    2007-01-01

    Full Text Available Early identification of dysplasia remains a critical goal for diagnostic endoscopy since early discovery directly improves patient survival because it allows endoscopic or surgical intervention with disease localized without lymph node involvement. Clinical studies have successfully used tissue autofluorescence with conventional white light endoscopy and biopsy for detecting adenomatous colonic polyps, differentiating benign hyperplastic from adenomas with acceptable sensitivity and specificity. In Barrett's esophagus, the detection of dysplasia remains problematic because of background inflammation, whereas in the squamous esophagus, autofluorescence imaging appears to be more dependable. Point fluorescence spectroscopy, although playing a crucial role in the pioneering mechanistic development of fluorescence endoscopic imaging, does not seem to have a current function in endoscopy because of its nontargeted sampling and suboptimal sensitivity and specificity. Other point spectroscopic modalities, such as Raman spectroscopy and elastic light scattering, continue to be evaluated in clinical studies, but still suffer the significant disadvantages of being random and nonimaging. A recent addition to the fluorescence endoscopic imaging arsenal is the use of confocal fluorescence endomicroscopy, which provides real-time optical biopsy for the first time. To improve detection of dysplasia in the gastrointestinal tract, a new and exciting development has been the use of exogenous fluorescence contrast probes that specifically target a variety of disease-related cellular biomarkers using conventional fluorescent dyes and novel potent fluorescent nanocrystals (i.e., quantum dots. This is an area of great promise, but still in its infancy, and preclinical studies are currently under way.

  6. Featured Article: Genotation: Actionable knowledge for the scientific reader.

    Science.gov (United States)

    Nagahawatte, Panduka; Willis, Ethan; Sakauye, Mark; Jose, Rony; Chen, Hao; Davis, Robert L

    2016-06-01

    We present an article viewer application that allows a scientific reader to easily discover and share knowledge by linking genomics-related concepts to knowledge of disparate biomedical databases. High-throughput data streams generated by technical advancements have contributed to scientific knowledge discovery at an unprecedented rate. Biomedical Informaticists have created a diverse set of databases to store and retrieve the discovered knowledge. The diversity and abundance of such resources present biomedical researchers a challenge with knowledge discovery. These challenges highlight a need for a better informatics solution. We use a text mining algorithm, Genomine, to identify gene symbols from the text of a journal article. The identified symbols are supplemented with information from the GenoDB knowledgebase. Self-updating GenoDB contains information from NCBI Gene, Clinvar, Medgen, dbSNP, KEGG, PharmGKB, Uniprot, and Hugo Gene databases. The journal viewer is a web application accessible via a web browser. The features described herein are accessible on www.genotation.org The Genomine algorithm identifies gene symbols with an accuracy shown by .65 F-Score. GenoDB currently contains information regarding 59,905 gene symbols, 5633 drug-gene relationships, 5981 gene-disease relationships, and 713 pathways. This application provides scientific readers with actionable knowledge related to concepts of a manuscript. The reader will be able to save and share supplements to be visualized in a graphical manner. This provides convenient access to details of complex biological phenomena, enabling biomedical researchers to generate novel hypothesis to further our knowledge in human health. This manuscript presents a novel application that integrates genomic, proteomic, and pharmacogenomic information to supplement content of a biomedical manuscript and enable readers to automatically discover actionable knowledge.

  7. Solar Energy Education. Reader, Part IV. Sun schooling

    Energy Technology Data Exchange (ETDEWEB)

    1981-05-01

    A collection of magazine articles which focus on solar energy is presented. This is the final book of the four part series of the Solar Energy Reader. The articles include brief discussions on energy topics such as the sun, ocean energy, methane gas from cow manure, and solar homes. Instructions for constructing a sundial and a solar stove are also included. A glossary of energy related terms is provided. (BCS)

  8. ELISA reader does not interfere by mobile phone radiofrequency radiation.

    Science.gov (United States)

    Mortazavi, Seyyed Mohammad Javad; Baradaran-Ghahfarokhi, Hamid Reza; Abdi, Mohammad Reza; Baradaran-Ghahfarokhi, Milad; Mostafavi, Nayyer Sadat; Mahmoudi, Golshan; Berenjkoub, Nafiseh; Akmali, Zahra; Hossein-Beigi, Fahimeh; Arsang, Vajiheh

    2016-01-01

    The increasing number of mobile phones can physically cause electromagnetic interference (EMI) in medical environments; can also cause errors in immunoassays in laboratories. The ELISA readers are widely used as a useful diagnostic tool for Enzymun colorimetric assay in medicine. The aim of this study was to investigate whether the ELISA reader could be interfered by the exposure to the 900 MHz cell phones in the laboratory. Human serum samples were collected from 14 healthy donors (9 women and 5 men) and each sample was divided into four aliquots and was placed into four batches for the in-vitro quantitative determination of human chorionic gonadotropin (hCG). During colorimetric reading of the first, second, and third batches, the ELISA reader (Stat Fax 2100, Awareness Technology, Inc., USA) was exposed to 0.5, 1.0, and 2.0 W exposure of 900 MHz radiation, respectively. For the forth batch (control group), no radiation was applied. All experiments were performed comparing ELISA read out results of the I, II, and III batches with the control batch, using the Wilcoxon test with criterion level of P = 0.050. The final scores in the exposed batches I, II, and III were not statistically significant relative to the control batch (P > 0.05). The results showed that 900 MHz radiation exposure did not alter the ELISA measured levels of hCG hormone in I (P = 0.219), II (P = 0.909), and III (P = 0.056) batches compared to the control batch. This study showed that ELISA reader does not interfere by mobile phone RF radiation at a closed contact (less than 5 cm distance). However, we recommend that medical institutions discuss these issues in the context of their specific use of technologies and frame a policy that is clear and straightforward to guide staff, patients, and visitors.

  9. The concept of locking plates.

    Science.gov (United States)

    Cronier, P; Pietu, G; Dujardin, C; Bigorre, N; Ducellier, F; Gerard, R

    2010-05-01

    After a short historical review of locking bone plates since their inception more than a century ago to the success of the concept less than 15 years ago with today's plates, the authors present the main locking mechanisms in use. In the two broad categories - plates with fixed angulation and those with variable angulation - the screw head is locked in the plate with a locknut by screwing in a threaded chamber on the plate or by screwing through an adapted ring. The authors then provide a concrete explanation, based on simple mechanical models, of the fundamental differences between conventional bone plates and locking plates and why a locking screw system presents greater resistance at disassembly, detailing the role played by the position and number of screws. The advantages of epiphyseal fixation are then discussed, including in cases of mediocre-quality bone. For teaching purposes, the authors also present assembly with an apple fixed with five locking screws withstanding a 47-kg axial load with no resulting disassembly. The principles of plate placement are detailed for both the epiphysis and diaphysis, including the number and position of screws and respect of the soft tissues, with the greatest success assured by the minimally invasive and even percutaneous techniques. The authors then present the advantages of locking plates in fixation of periprosthetic fractures where conventional osteosynthesis often encounters limited success. Based on simplified theoretical cases, the economic impact in France of this type of implant is discussed, showing that on average it accounts for less than 10% of the overall cost of this pathology to society. Finally, the possible problems of material ablation are discussed as well as the means to remediate these problems.

  10. Social  reading - the reader on digital margins

    Directory of Open Access Journals (Sweden)

    Ivona Despot

    2016-12-01

    Full Text Available Electronic books enrich the reading experience through a range of possibilities digital technology offers, such as commenting or adding content at the margin space, marking interesting chapters and sharing the content with other readers. The phenomenon of social reading emerges with the influence of technology in the sphere of reading books and creates interactions for readers with the content and other readers. The great potential of enriching the reading experience is visible in the digital platforms for social reading, where the interaction and the creation of new content encourages the development of new way of reading and creativity. These activities may contribute to better understanding of the text. This enhances the communication about the text thus revitalizing the content and moves the reading itself from private to public sphere. This paper will show how are the activities in the digital margins transformed into a good indicator of the reading behavior, as well as the importance and usefulness of such for publishers to create new publishing products and services.

  11. Modeling Reader's Emotional State Response on Document's Typographic Elements

    Directory of Open Access Journals (Sweden)

    Dimitrios Tsonos

    2011-01-01

    Full Text Available We present the results of an experimental study towards modeling the reader's emotional state variations induced by the typographic elements in electronic documents. Based on the dimensional theory of emotions we investigate how typographic elements, like font style (bold, italics, bold-italics and font (type, size, color and background color, affect the reader's emotional states, namely, Pleasure, Arousal, and Dominance (PAD. An experimental procedure was implemented conforming to International Affective Picture System guidelines and incorporating the Self-Assessment Manikin test. Thirty students participated in the experiment. The stimulus was a short paragraph of text for which any content, emotion, and/or domain dependent information was excluded. The Analysis of Variance revealed the dependency of (a all the three emotional dimensions on font size and font/background color combinations and (b the Pleasure dimension on font type and font style. We introduce a set of mapping rules showing how PAD vary on the discrete values of font style and font type elements. Moreover, we introduce a set of equations describing the PAD dimensions' dependency on font size. This novel model can contribute to the automated reader's emotional state extraction in order, for example, to enhance the acoustic rendition of the documents, utilizing text-to-speech synthesis.

  12. 给Acrobat Reader 加书签

    Institute of Scientific and Technical Information of China (English)

    段晓东

    2012-01-01

    Acrobat Reader是一个PDF格式阅读器,但是不带书签功能是它的一个硬伤,害得好多同学每次看完书要记住当天阅读的页码,麻烦至极。其实只要下载bookmark_page.js-1.0.zip这个小小文件,这些烦恼即可解决。 第一步:将压缩包中的bookmark_page.js解压到X:/Program Files/Adobe/Reader X.0/Reader/Javascripts目录下(其中X为程序所在的分区盘符,要特别注意的是随Acrobat Reader版本不同复制位置有可能不同,例如对9.0而言,我的复制全路径是D:/Program files/Adobe/Reader9.0/Reader/javascripts),但无论如何请记住只需将解压文件复制到这个Javascripts文件夹即可。(剩余208字)

  13. STARtorialist: Astronomy Fashion & Culture Blog and Reader Survey Results

    Science.gov (United States)

    Ash, Summer; Rice, Emily L.; Jarreau, Paige B.

    2016-01-01

    STARtorialist (startorialist.com) is a Tumblr-based blog that curates the proliferation of "Astro Fashion" - clothing accessories, decor, and more - with the goal of celebrating the beauty of the universe and highlighting the science behind the astronomical imagery. Since launching in January 2013, we have written over 1000 unique posts about everything from handmade and boutique products to mass-produced commercial items to haute couture seen on fashion runways. Each blog post features images and descriptions of the products with links to the original astronomical images or other relevant science content. We also feature profiles of astronomers, scientists, students, and communicators wearing, making, or decorating with "startorial" items. Our most popular posts accumulate hundreds or thousands of notes (faves or reblogs, in Tumblr parlance), and one post has nearly 150,000 notes. In our second year, we have grown from 1,000 to just shy of 20,000 followers on Tumblr, with an increased audience on Twitter, Instagram, and Facebook as well. We present preliminary results from a reader survey conducted September-October 2015 in collaboration with science communication researcher Dr. Paige Jarreau. The survey provides data on reader habits, motivations, attitudes, and demographics in order to assess how STARtorialist has influenced our readers' views on science, scientists, and the scientific community as a whole.

  14. Text Mining in Python through the HTRC Feature Reader

    Directory of Open Access Journals (Sweden)

    Peter Organisciak

    2016-11-01

    Full Text Available We introduce a toolkit for working with the 13.6 million volume Extracted Features Dataset from the HathiTrust Research Center. You will learn how to peer at the words and trends of any book in the collection, while developing broadly useful Python data analysis skills. The HathiTrust holds nearly 15 million digitized volumes from libraries around the world. In addition to their individual value, these works in aggregate are extremely valuable for historians. Spanning many centuries and genres, they offer a way to learn about large-scale trends in history and culture, as well as evidence for changes in language or even the structure of the book. To simplify access to this collection the HathiTrust Research Center (HTRC has released the Extracted Features dataset (Capitanu et al. 2015: a dataset that provides quantitative information describing every page of every volume in the collection. In this lesson, we introduce the HTRC Feature Reader, a library for working with the HTRC Extracted Features dataset using the Python programming language. The HTRC Feature Reader is structured to support work using popular data science libraries, particularly Pandas. Pandas provides simple structures for holding data and powerful ways to interact with it. The HTRC Feature Reader uses these data structures, so learning how to use it will also cover general data analysis skills in Python.

  15. Highly thermostable fluorescent proteins

    Science.gov (United States)

    Bradbury, Andrew M [Santa Fe, NM; Waldo, Geoffrey S [Santa Fe, NM; Kiss, Csaba [Los Alamos, NM

    2012-05-01

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  16. Highly thermostable fluorescent proteins

    Science.gov (United States)

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  17. The Writing Teacher as a Dumb Reader: Some Notes on Clarity.

    Science.gov (United States)

    Gibson, Walker

    Readers are "dumb" because they are not privy to the mind and intentions of the writer; and the failure of the unsuccessful writer is a failure to forecast what it is going to be like to be a dumb reader of the document. Sample sentences from students' writing illustrate the following types of writing problems, which force the reader to examine…

  18. Reading Logs: An Application of Reader-Response Theory in ELT.

    Science.gov (United States)

    Carlisle, Anthony

    2000-01-01

    Introduces the activity of student-written reading logs as a practical application of reader-response theory in English-as-Foreign-Language literature teaching. Because reader-response theory stresses the synthesis between reader and text, it is proposed that practical applications should be based on the interaction. (Author/VWL)

  19. Reader-Response Theory and Instructors' Holistic Evaluating in and out of Their Fields.

    Science.gov (United States)

    Anderson, Larry; And Others

    1994-01-01

    Claims that holistic scoring for schoolwide assessment of writing proficiency is influenced by the disciplines represented by the faculty readers. Describes in detail the findings of a study designed to measure reader bias in evaluation, a phenomenon suggested by reader-response theory. (HB)

  20. Limits of Identification: The Personal, Pleasurable, and Critical in Reader Response.

    Science.gov (United States)

    Lewis, Cynthia

    2000-01-01

    Argues that the most common use of reader-response theory in the classroom is misguided in its emphasis on personal response and identification. Discusses the social and political nature of readers, texts, and contexts. Suggests that when a text is about characters whose life and culture are very different from the reader's, it can heighten the…