Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity

Energy Technology Data Exchange (ETDEWEB)

Schorb, Martin [Structural and Computational Biology Unit, European Molecular Biology Laboratory, D-69117 Heidelberg (Germany); Briggs, John A.G., E-mail: john.briggs@embl.de [Structural and Computational Biology Unit, European Molecular Biology Laboratory, D-69117 Heidelberg (Germany); Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, D-69117 Heidelberg (Germany)

2014-08-01

Performing fluorescence microscopy and electron microscopy on the same sample allows fluorescent signals to be used to identify and locate features of interest for subsequent imaging by electron microscopy. To carry out such correlative microscopy on vitrified samples appropriate for structural cryo-electron microscopy it is necessary to perform fluorescence microscopy at liquid-nitrogen temperatures. Here we describe an adaptation of a cryo-light microscopy stage to permit use of high-numerical aperture objectives. This allows high-sensitivity and high-resolution fluorescence microscopy of vitrified samples. We describe and apply a correlative cryo-fluorescence and cryo-electron microscopy workflow together with a fiducial bead-based image correlation procedure. This procedure allows us to locate fluorescent bacteriophages in cryo-electron microscopy images with an accuracy on the order of 50 nm, based on their fluorescent signal. It will allow the user to precisely and unambiguously identify and locate objects and events for subsequent high-resolution structural study, based on fluorescent signals. - Highlights: • Workflow for correlated cryo-fluorescence and cryo-electron microscopy. • Cryo-fluorescence microscopy setup incorporating a high numerical aperture objective. • Fluorescent signals located in cryo-electron micrographs with 50 nm spatial precision.

A new methodology of spatial cross-correlation analysis.

Science.gov (United States)

Chen, Yanguang

2015-01-01

Spatial correlation modeling comprises both spatial autocorrelation and spatial cross-correlation processes. The spatial autocorrelation theory has been well-developed. It is necessary to advance the method of spatial cross-correlation analysis to supplement the autocorrelation analysis. This paper presents a set of models and analytical procedures for spatial cross-correlation analysis. By analogy with Moran's index newly expressed in a spatial quadratic form, a theoretical framework is derived for geographical cross-correlation modeling. First, two sets of spatial cross-correlation coefficients are defined, including a global spatial cross-correlation coefficient and local spatial cross-correlation coefficients. Second, a pair of scatterplots of spatial cross-correlation is proposed, and the plots can be used to visually reveal the causality behind spatial systems. Based on the global cross-correlation coefficient, Pearson's correlation coefficient can be decomposed into two parts: direct correlation (partial correlation) and indirect correlation (spatial cross-correlation). As an example, the methodology is applied to the relationships between China's urbanization and economic development to illustrate how to model spatial cross-correlation phenomena. This study is an introduction to developing the theory of spatial cross-correlation, and future geographical spatial analysis might benefit from these models and indexes.

A New Methodology of Spatial Cross-Correlation Analysis

Science.gov (United States)

Chen, Yanguang

2015-01-01

Spatial correlation modeling comprises both spatial autocorrelation and spatial cross-correlation processes. The spatial autocorrelation theory has been well-developed. It is necessary to advance the method of spatial cross-correlation analysis to supplement the autocorrelation analysis. This paper presents a set of models and analytical procedures for spatial cross-correlation analysis. By analogy with Moran’s index newly expressed in a spatial quadratic form, a theoretical framework is derived for geographical cross-correlation modeling. First, two sets of spatial cross-correlation coefficients are defined, including a global spatial cross-correlation coefficient and local spatial cross-correlation coefficients. Second, a pair of scatterplots of spatial cross-correlation is proposed, and the plots can be used to visually reveal the causality behind spatial systems. Based on the global cross-correlation coefficient, Pearson’s correlation coefficient can be decomposed into two parts: direct correlation (partial correlation) and indirect correlation (spatial cross-correlation). As an example, the methodology is applied to the relationships between China’s urbanization and economic development to illustrate how to model spatial cross-correlation phenomena. This study is an introduction to developing the theory of spatial cross-correlation, and future geographical spatial analysis might benefit from these models and indexes. PMID:25993120

Mixed-correlated ARFIMA processes for power-law cross-correlations

Czech Academy of Sciences Publication Activity Database

Krištoufek, Ladislav

2013-01-01

Roč. 392, č. 24 (2013), s. 6484-6493 ISSN 0378-4371 R&D Projects: GA ČR GA402/09/0965 Institutional support: RVO:67985556 Keywords : power-law cross-correlations * long - term memory * econophysics Subject RIV: AH - Economics Impact factor: 1.722, year: 2013 http://library.utia.cas.cz/separaty/2013/E/kristoufek-mixed-correlated arfima processes for power-law cross-correlations.pdf

Multifractal temporally weighted detrended cross-correlation analysis to quantify power-law cross-correlation and its application to stock markets

Science.gov (United States)

Wei, Yun-Lan; Yu, Zu-Guo; Zou, Hai-Long; Anh, Vo

2017-06-01

A new method—multifractal temporally weighted detrended cross-correlation analysis (MF-TWXDFA)—is proposed to investigate multifractal cross-correlations in this paper. This new method is based on multifractal temporally weighted detrended fluctuation analysis and multifractal cross-correlation analysis (MFCCA). An innovation of the method is applying geographically weighted regression to estimate local trends in the nonstationary time series. We also take into consideration the sign of the fluctuations in computing the corresponding detrended cross-covariance function. To test the performance of the MF-TWXDFA algorithm, we apply it and the MFCCA method on simulated and actual series. Numerical tests on artificially simulated series demonstrate that our method can accurately detect long-range cross-correlations for two simultaneously recorded series. To further show the utility of MF-TWXDFA, we apply it on time series from stock markets and find that power-law cross-correlation between stock returns is significantly multifractal. A new coefficient, MF-TWXDFA cross-correlation coefficient, is also defined to quantify the levels of cross-correlation between two time series.

Fluorescence correlation spectroscopy to study antibody binding and stoichiometry of complexes

Science.gov (United States)

Swift, Kerry M.; Matayoshi, Edmund D.

2008-02-01

FCS (fluorescence correlation spectroscopy) was used to study the association at the single molecule level of tumor necrosis factor alpha (TNF-α) and two of its protein antagonists Humira (TM) (adalimumab), a fully humanized monoclonal antibody, and Enbrel (TM) (etanercept), a soluble form of the TNF receptor. Single molecule approaches potentially have the advantage not only of enhanced sensitivity, but also of observing at equilibrium the details that would otherwise be lost in classical ensemble experiments where heterogeneity is averaged. We prepared fluorescent conjugates of the protein drugs and their biological target, the trimeric soluble form of TNF-α. The bivalency of adalimumab and the trimeric nature of TNF-α potentially allow several forms of associative complexes that may differ in stoichiometry. Detailed knowledge of this reaction may be relevant to understanding adalimumab's pharmacological properties. Our FCS data showed that a single trimeric TNF-α can bind up to three adalimumab molecules. Under some conditions even larger complexes are formed, apparently the result of cross-linking of TNF-α trimers by adalimumab. In addition, distinct differences between Humira and Enbrel were observed in their association with TNF-α.

Visualization of synchronization of the uterine contraction signals: running cross-correlation and wavelet running cross-correlation methods.

Science.gov (United States)

Oczeretko, Edward; Swiatecka, Jolanta; Kitlas, Agnieszka; Laudanski, Tadeusz; Pierzynski, Piotr

2006-01-01

In physiological research, we often study multivariate data sets, containing two or more simultaneously recorded time series. The aim of this paper is to present the cross-correlation and the wavelet cross-correlation methods to assess synchronization between contractions in different topographic regions of the uterus. From a medical point of view, it is important to identify time delays between contractions, which may be of potential diagnostic significance in various pathologies. The cross-correlation was computed in a moving window with a width corresponding to approximately two or three contractions. As a result, the running cross-correlation function was obtained. The propagation% parameter assessed from this function allows quantitative description of synchronization in bivariate time series. In general, the uterine contraction signals are very complicated. Wavelet transforms provide insight into the structure of the time series at various frequencies (scales). To show the changes of the propagation% parameter along scales, a wavelet running cross-correlation was used. At first, the continuous wavelet transforms as the uterine contraction signals were received and afterwards, a running cross-correlation analysis was conducted for each pair of transformed time series. The findings show that running functions are very useful in the analysis of uterine contractions.

Multiscale Detrended Cross-Correlation Analysis of STOCK Markets

Science.gov (United States)

Yin, Yi; Shang, Pengjian

2014-06-01

In this paper, we employ the detrended cross-correlation analysis (DCCA) to investigate the cross-correlations between different stock markets. We report the results of cross-correlated behaviors in US, Chinese and European stock markets in period 1997-2012 by using DCCA method. The DCCA shows the cross-correlated behaviors of intra-regional and inter-regional stock markets in the short and long term which display the similarities and differences of cross-correlated behaviors simply and roughly and the persistence of cross-correlated behaviors of fluctuations. Then, because of the limitation and inapplicability of DCCA method, we propose multiscale detrended cross-correlation analysis (MSDCCA) method to avoid "a priori" selecting the ranges of scales over which two coefficients of the classical DCCA method are identified, and employ MSDCCA to reanalyze these cross-correlations to exhibit some important details such as the existence and position of minimum, maximum and bimodal distribution which are lost if the scale structure is described by two coefficients only and essential differences and similarities in the scale structures of cross-correlation of intra-regional and inter-regional markets. More statistical characteristics of cross-correlation obtained by MSDCCA method help us to understand how two different stock markets influence each other and to analyze the influence from thus two inter-regional markets on the cross-correlation in detail, thus we get a richer and more detailed knowledge of the complex evolutions of dynamics of the cross-correlations between stock markets. The application of MSDCCA is important to promote our understanding of the internal mechanisms and structures of financial markets and helps to forecast the stock indices based on our current results demonstrated the cross-correlations between stock indices. We also discuss the MSDCCA methods of secant rolling window with different sizes and, lastly, provide some relevant implications and

Cross-correlations and influence in world gold markets

Science.gov (United States)

Lin, Min; Wang, Gang-Jin; Xie, Chi; Stanley, H. Eugene

2018-01-01

Using the detrended cross-correlation analysis (DCCA) coefficient and the detrended partial cross-correlation analysis (DPCCA) coefficient, we investigate cross-correlations and net cross-correlations among five major world gold markets (London, New York, Shanghai, Tokyo, and Mumbai) at different time scales. We propose multiscale influence measures for examining the influence of individual markets on other markets and on the entire system. We find (i) that the cross-correlations, net cross-correlations, and net influences among the five gold markets vary across time scales, (ii) that the cross-market correlation between London and New York at each time scale is intense and inherent, meaning that the influence of other gold markets on the London-New York market is negligible, (iii) that the remaining cross-market correlations (i.e., those other than London-New York) are greatly affected by other gold markets, and (iv) that the London gold market significantly affects the other four gold markets and dominates the world-wide gold market. Our multiscale findings give market participants and market regulators new information on cross-market linkages in the world-wide gold market.

Two-dimensional multifractal cross-correlation analysis

International Nuclear Information System (INIS)

Xi, Caiping; Zhang, Shuning; Xiong, Gang; Zhao, Huichang; Yang, Yonghong

2017-01-01

Highlights: • We study the mathematical models of 2D-MFXPF, 2D-MFXDFA and 2D-MFXDMA. • Present the definition of the two-dimensional N 2 -partitioned multiplicative cascading process. • Do the comparative analysis of 2D-MC by 2D-MFXPF, 2D-MFXDFA and 2D-MFXDMA. • Provide a reference on the choice and parameter settings of these methods in practice. - Abstract: There are a number of situations in which several signals are simultaneously recorded in complex systems, which exhibit long-term power-law cross-correlations. This paper presents two-dimensional multifractal cross-correlation analysis based on the partition function (2D-MFXPF), two-dimensional multifractal cross-correlation analysis based on the detrended fluctuation analysis (2D-MFXDFA) and two-dimensional multifractal cross-correlation analysis based on the detrended moving average analysis (2D-MFXDMA). We apply these methods to pairs of two-dimensional multiplicative cascades (2D-MC) to do a comparative study. Then, we apply the two-dimensional multifractal cross-correlation analysis based on the detrended fluctuation analysis (2D-MFXDFA) to real images and unveil intriguing multifractality in the cross correlations of the material structures. At last, we give the main conclusions and provide a valuable reference on how to choose the multifractal algorithms in the potential applications in the field of SAR image classification and detection.

Detrended cross-correlation analysis of electroencephalogram

International Nuclear Information System (INIS)

Wang Jun; Zhao Da-Qing

2012-01-01

In the paper we use detrended cross-correlation analysis (DCCA) to study the electroencephalograms of healthy young subjects and healthy old subjects. It is found that the cross-correlation between different leads of a healthy young subject is larger than that of a healthy old subject. It was shown that the cross-correlation relationship decreases with the aging process and the phenomenon can help to diagnose whether the subject's brain function is healthy or not. (interdisciplinary physics and related areas of science and technology)

Random matrix theory analysis of cross-correlations in the US stock market: Evidence from Pearson’s correlation coefficient and detrended cross-correlation coefficient

Science.gov (United States)

Wang, Gang-Jin; Xie, Chi; Chen, Shou; Yang, Jiao-Jiao; Yang, Ming-Yan

2013-09-01

In this study, we first build two empirical cross-correlation matrices in the US stock market by two different methods, namely the Pearson’s correlation coefficient and the detrended cross-correlation coefficient (DCCA coefficient). Then, combining the two matrices with the method of random matrix theory (RMT), we mainly investigate the statistical properties of cross-correlations in the US stock market. We choose the daily closing prices of 462 constituent stocks of S&P 500 index as the research objects and select the sample data from January 3, 2005 to August 31, 2012. In the empirical analysis, we examine the statistical properties of cross-correlation coefficients, the distribution of eigenvalues, the distribution of eigenvector components, and the inverse participation ratio. From the two methods, we find some new results of the cross-correlations in the US stock market in our study, which are different from the conclusions reached by previous studies. The empirical cross-correlation matrices constructed by the DCCA coefficient show several interesting properties at different time scales in the US stock market, which are useful to the risk management and optimal portfolio selection, especially to the diversity of the asset portfolio. It will be an interesting and meaningful work to find the theoretical eigenvalue distribution of a completely random matrix R for the DCCA coefficient because it does not obey the Marčenko-Pastur distribution.

Quantification of leakage from large unilamellar lipid vesicles by fluorescence correlation spectroscopy

DEFF Research Database (Denmark)

Kristensen, Kasper; Henriksen, Jonas Rosager; Andresen, Thomas Lars

2014-01-01

Fluorescence correlation spectroscopy (FCS) is a powerful experimental technique that in recent years has found numerous applications for studying biological phenomena. In this article, we scrutinize one of these applications, namely, FCS as a technique for studying leakage of fluorescent molecul...

Analyzing the Cross-Correlation Between Onshore and Offshore RMB Exchange Rates Based on Multifractal Detrended Cross-Correlation Analysis (MF-DCCA)

Science.gov (United States)

Xie, Chi; Zhou, Yingying; Wang, Gangjin; Yan, Xinguo

We use the multifractal detrended cross-correlation analysis (MF-DCCA) method to explore the multifractal behavior of the cross-correlation between exchange rates of onshore RMB (CNY) and offshore RMB (CNH) against US dollar (USD). The empirical data are daily prices of CNY/USD and CNH/USD from May 1, 2012 to February 29, 2016. The results demonstrate that: (i) the cross-correlation between CNY/USD and CNH/USD is persistent and its fluctuation is smaller when the order of fluctuation function is negative than that when the order is positive; (ii) the multifractal behavior of the cross-correlation between CNY/USD and CNH/USD is significant during the sample period; (iii) the dynamic Hurst exponents obtained by the rolling windows analysis show that the cross-correlation is stable when the global economic situation is good and volatile in bad situation; and (iv) the non-normal distribution of original data has a greater effect on the multifractality of the cross-correlation between CNY/USD and CNH/USD than the temporary correlation.

Flow velocity measurement by using zero-crossing polarity cross correlation method

International Nuclear Information System (INIS)

Xu Chengji; Lu Jinming; Xia Hong

1993-01-01

Using the designed correlation metering system and a high accurate hot-wire anemometer as a calibration device, the experimental study of correlation method in a tunnel was carried out. The velocity measurement of gas flow by using zero-crossing polarity cross correlation method was realized and the experimental results has been analysed

Membrane mobility and microdomain association of the dopaminetransporter studied with fluorescence correlation spectroscopy and fluorescence recovery after photobleaching

DEFF Research Database (Denmark)

Adkins, Erika; Samuvel, Devadoss; Fog, Jacob

2007-01-01

To investigate microdomain association of the dopamine transporter (DAT), we employed FCS (fluorescence correlation spectroscopy) and FRAP (fluorescence recovery after photobleaching). In non-neuronal cells (HEK293), FCS measurements revealed for the YFP-DAT (DAT tagged with yellow fluorescent...... protein) a diffusion coefficient (D) of ~3.6 × 10-9 cm2/s, consistent with a relatively freely diffusible protein. In neuronally derived cells (N2a), we were unable to perform FCS measurements on plasma membrane-associated protein due to photobleaching, suggesting partial immobilization...

Multifractal detrended cross-correlation analysis in the MENA area

Science.gov (United States)

El Alaoui, Marwane; Benbachir, Saâd

2013-12-01

In this paper, we investigated multifractal cross-correlations qualitatively and quantitatively using a cross-correlation test and the Multifractal detrended cross-correlation analysis method (MF-DCCA) for markets in the MENA area. We used cross-correlation coefficients to measure the level of this correlation. The analysis concerns four stock market indices of Morocco, Tunisia, Egypt and Jordan. The countries chosen are signatory of the Agadir agreement concerning the establishment of a free trade area comprising Arab Mediterranean countries. We computed the bivariate generalized Hurst exponent, Rényi exponent and spectrum of singularity for each pair of indices to measure quantitatively the cross-correlations. By analyzing the results, we found the existence of multifractal cross-correlations between all of these markets. We compared the spectrum width of these indices; we also found which pair of indices has a strong multifractal cross-correlation.

Testing Cross-Sectional Correlation in Large Panel Data Models with Serial Correlation

Directory of Open Access Journals (Sweden)

Badi H. Baltagi

2016-11-01

Full Text Available This paper considers the problem of testing cross-sectional correlation in large panel data models with serially-correlated errors. It finds that existing tests for cross-sectional correlation encounter size distortions with serial correlation in the errors. To control the size, this paper proposes a modification of Pesaran’s Cross-sectional Dependence (CD test to account for serial correlation of an unknown form in the error term. We derive the limiting distribution of this test as N , T → ∞ . The test is distribution free and allows for unknown forms of serial correlation in the errors. Monte Carlo simulations show that the test has good size and power for large panels when serial correlation in the errors is present.

Multifractal Detrended Cross-Correlation Analysis of agricultural futures markets

International Nuclear Information System (INIS)

He Lingyun; Chen Shupeng

2011-01-01

Highlights: → We investigated cross-correlations between China's and US agricultural futures markets. → Power-law cross-correlations are found between the geographically far but correlated markets. → Multifractal features are significant in all the markets. → Cross-correlation exponent is less than averaged GHE when q 0. - Abstract: We investigated geographically far but temporally correlated China's and US agricultural futures markets. We found that there exists a power-law cross-correlation between them, and that multifractal features are significant in all the markets. It is very interesting that the geographically far markets show strong cross-correlations and share much of their multifractal structure. Furthermore, we found that for all the agricultural futures markets in our studies, the cross-correlation exponent is less than the averaged generalized Hurst exponents (GHE) when q 0.

Correlated quadratures of resonance fluorescence and the generalized uncertainty relation

Science.gov (United States)

Arnoldus, Henk F.; George, Thomas F.; Gross, Rolf W. F.

1994-01-01

Resonance fluorescence from a two-state atom has been predicted to exhibit quadrature squeezing below the Heisenberg uncertainty limit, provided that the optical parameters (Rabi frequency, detuning, laser linewidth, etc.) are chosen carefully. When the correlation between two quadratures of the radiation field does not vanish, however, the Heisenberg limit for quantum fluctuations might be an unrealistic lower bound. A generalized uncertainty relation, due to Schroedinger, takes into account the possible correlation between the quadrature components of the radiation, and it suggests a modified definition of squeezing. We show that the coherence between the two levels of a laser-driven atom is responsible for the correlation between the quadrature components of the emitted fluorescence, and that the Schrodinger uncertainty limit increases monotonically with the coherence. On the other hand, the fluctuations in the quadrature field diminish with an increasing coherence, and can disappear completely when the coherence reaches 1/2, provided that certain phase relations hold.

Statistical tests for power-law cross-correlated processes

Science.gov (United States)

Podobnik, Boris; Jiang, Zhi-Qiang; Zhou, Wei-Xing; Stanley, H. Eugene

2011-12-01

For stationary time series, the cross-covariance and the cross-correlation as functions of time lag n serve to quantify the similarity of two time series. The latter measure is also used to assess whether the cross-correlations are statistically significant. For nonstationary time series, the analogous measures are detrended cross-correlations analysis (DCCA) and the recently proposed detrended cross-correlation coefficient, ρDCCA(T,n), where T is the total length of the time series and n the window size. For ρDCCA(T,n), we numerically calculated the Cauchy inequality -1≤ρDCCA(T,n)≤1. Here we derive -1≤ρDCCA(T,n)≤1 for a standard variance-covariance approach and for a detrending approach. For overlapping windows, we find the range of ρDCCA within which the cross-correlations become statistically significant. For overlapping windows we numerically determine—and for nonoverlapping windows we derive—that the standard deviation of ρDCCA(T,n) tends with increasing T to 1/T. Using ρDCCA(T,n) we show that the Chinese financial market's tendency to follow the U.S. market is extremely weak. We also propose an additional statistical test that can be used to quantify the existence of cross-correlations between two power-law correlated time series.

Correlative cryo-fluorescence light microscopy and cryo-electron tomography of Streptomyces.

Science.gov (United States)

Koning, Roman I; Celler, Katherine; Willemse, Joost; Bos, Erik; van Wezel, Gilles P; Koster, Abraham J

2014-01-01

Light microscopy and electron microscopy are complementary techniques that in a correlative approach enable identification and targeting of fluorescently labeled structures in situ for three-dimensional imaging at nanometer resolution. Correlative imaging allows electron microscopic images to be positioned in a broader temporal and spatial context. We employed cryo-correlative light and electron microscopy (cryo-CLEM), combining cryo-fluorescence light microscopy and cryo-electron tomography, on vitrified Streptomyces bacteria to study cell division. Streptomycetes are mycelial bacteria that grow as long hyphae and reproduce via sporulation. On solid media, Streptomyces subsequently form distinct aerial mycelia where cell division leads to the formation of unigenomic spores which separate and disperse to form new colonies. In liquid media, only vegetative hyphae are present divided by noncell separating crosswalls. Their multicellular life style makes them exciting model systems for the study of bacterial development and cell division. Complex intracellular structures have been visualized with transmission electron microscopy. Here, we describe the methods for cryo-CLEM that we applied for studying Streptomyces. These methods include cell growth, fluorescent labeling, cryo-fixation by vitrification, cryo-light microscopy using a Linkam cryo-stage, image overlay and relocation, cryo-electron tomography using a Titan Krios, and tomographic reconstruction. Additionally, methods for segmentation, volume rendering, and visualization of the correlative data are described. © 2014 Elsevier Inc. All rights reserved.

Bunches of random cross-correlated sequences

International Nuclear Information System (INIS)

Maystrenko, A A; Melnik, S S; Pritula, G M; Usatenko, O V

2013-01-01

The statistical properties of random cross-correlated sequences constructed by the convolution method (likewise referred to as the Rice or the inverse Fourier transformation) are examined. We clarify the meaning of the filtering function—the kernel of the convolution operator—and show that it is the value of the cross-correlation function which describes correlations between the initial white noise and constructed correlated sequences. The matrix generalization of this method for constructing a bunch of N cross-correlated sequences is presented. Algorithms for their generation are reduced to solving the problem of decomposition of the Fourier transform of the correlation matrix into a product of two mutually conjugate matrices. Different decompositions are considered. The limits of weak and strong correlations for the one-point probability and pair correlation functions of sequences generated by the method under consideration are studied. Special cases of heavy-tailed distributions of the generated sequences are analyzed. We show that, if the filtering function is rather smooth, the distribution function of generated variables has the Gaussian or Lévy form depending on the analytical properties of the distribution (or characteristic) functions of the initial white noise. Anisotropic properties of statistically homogeneous random sequences related to the asymmetry of a filtering function are revealed and studied. These asymmetry properties are expressed in terms of the third- or fourth-order correlation functions. Several examples of the construction of correlated chains with a predefined correlation matrix are given. (paper)

Membrane mobility and microdomain association of the dopamine transporter studied with fluorescence correlation spectroscopy and fluorescence recovery after photobleaching

DEFF Research Database (Denmark)

Adkins, Erika M; Samuvel, Devadoss J; Fog, Jacob U

2007-01-01

To investigate microdomain association of the dopamine transporter (DAT), we employed FCS (fluorescence correlation spectroscopy) and FRAP (fluorescence recovery after photobleaching). In non-neuronal cells (HEK293), FCS measurements revealed for the YFP-DAT (DAT tagged with yellow fluorescent...... protein) a diffusion coefficient (D) of approximately 3.6 x 10(-9) cm2/s, consistent with a relatively freely diffusible protein. In neuronally derived cells (N2a), we were unable to perform FCS measurements on plasma membrane-associated protein due to photobleaching, suggesting partial immobilization...

Stochastic systems with cross-correlated Gaussian white noises

International Nuclear Information System (INIS)

Wang Cheng-Yu; Song Yu-Min; Zhou Peng; Yang Hai; Gao Yun

2010-01-01

This paper theoretically investigates three stochastic systems with cross-correlation Gaussian white noises. Both steady state properties of the stochastic nonlinear systems and the nonequilibrium transitions induced by the cross-correlated noises are studied. The stationary solutions of the Fokker—Planck equation for three specific examples are analysed. It is shown explicitly that the cross-correlation of white noises can induce nonequilibrium transitions

Cross-correlation of long-range correlated series

International Nuclear Information System (INIS)

Arianos, Sergio; Carbone, Anna

2009-01-01

A method for estimating the cross-correlation C xy (τ) of long-range correlated series x(t) and y(t), at varying lags τ and scales n, is proposed. For fractional Brownian motions with Hurst exponents H 1 and H 2 , the asymptotic expression for C xy (τ) depends only on the lag τ (wide-sense stationarity) and scales as a power of n with exponent H 1 +H 2 for τ→0. The method is illustrated on: (i) financial series, to show the leverage effect; (ii) genomic sequences, to estimate the correlations between structural parameters along the chromosomes

Commercial counterboard for 10 ns software correlator for photon and fluorescence correlation spectroscopy

Science.gov (United States)

Molteni, Matteo; Ferri, Fabio

2016-11-01

A 10 ns time resolution, multi-tau software correlator, capable of computing simultaneous autocorrelation (A-A, B-B) and cross (A-B) correlation functions at count rates up to ˜10 MHz, with no data loss, has been developed in LabVIEW and C++ by using the National Instrument timer/counterboard (NI PCIe-6612) and a fast Personal Computer (PC) (Intel Core i7-4790 Processor 3.60 GHz ). The correlator works by using two algorithms: for large lag times (τ ≳ 1 μs), a classical time-mode scheme, based on the measure of the number of pulses per time interval, is used; differently, for τ ≲ 1 μs a photon-mode (PM) scheme is adopted and the correlation function is retrieved from the sequence of the photon arrival times. Single auto- and cross-correlation functions can be processed online in full real time up to count rates of ˜1.8 MHz and ˜1.2 MHz, respectively. Two autocorrelation (A-A, B-B) and a cross correlation (A-B) functions can be simultaneously processed in full real time only up to count rates of ˜750 kHz. At higher count rates, the online processing takes place in a delayed modality, but with no data loss. When tested with simulated correlation data and latex spheres solutions, the overall performances of the correlator appear to be comparable with those of commercial hardware correlators, but with several nontrivial advantages related to its flexibility, low cost, and easy adaptability to future developments of PC and data acquisition technology.

Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds.

Science.gov (United States)

Peng, Sijia; Wang, Wenjuan; Chen, Chunlai

2018-05-10

Fluorescence correlation spectroscopy is a powerful single-molecule tool that is able to capture kinetic processes occurring at the nanosecond time scale. However, the upper limit of its time window is restricted by the dwell time of the molecule of interest in the confocal detection volume, which is usually around submilliseconds for a freely diffusing biomolecule. Here, we present a simple and easy-to-implement method, named surface transient binding-based fluorescence correlation spectroscopy (STB-FCS), which extends the upper limit of the time window to seconds. We further demonstrated that STB-FCS enables capture of both intramolecular and intermolecular kinetic processes whose time scales cross several orders of magnitude.

Signal Digitizer and Cross-Correlation Application Specific Integrated Circuit

Science.gov (United States)

Baranauskas, Dalius (Inventor); Baranauskas, Gytis (Inventor); Zelenin, Denis (Inventor); Kangaslahti, Pekka (Inventor); Tanner, Alan B. (Inventor); Lim, Boon H. (Inventor)

2017-01-01

According to one embodiment, a cross-correlator comprises a plurality of analog front ends (AFEs), a cross-correlation circuit and a data serializer. Each of the AFEs comprises a variable gain amplifier (VGA) and a corresponding analog-to-digital converter (ADC) in which the VGA receives and modifies a unique analog signal associates with a measured analog radio frequency (RF) signal and the ADC produces digital data associated with the modified analog signal. Communicatively coupled to the AFEs, the cross-correlation circuit performs a cross-correlation operation on the digital data produced from different measured analog RF signals. The data serializer is communicatively coupled to the summing and cross-correlating matrix and continuously outputs a prescribed amount of the correlated digital data.

ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy.

Science.gov (United States)

Brama, Elisabeth; Peddie, Christopher J; Wilkes, Gary; Gu, Yan; Collinson, Lucy M; Jones, Martin L

2016-12-13

In-resin fluorescence (IRF) protocols preserve fluorescent proteins in resin-embedded cells and tissues for correlative light and electron microscopy, aiding interpretation of macromolecular function within the complex cellular landscape. Dual-contrast IRF samples can be imaged in separate fluorescence and electron microscopes, or in dual-modality integrated microscopes for high resolution correlation of fluorophore to organelle. IRF samples also offer a unique opportunity to automate correlative imaging workflows. Here we present two new locator tools for finding and following fluorescent cells in IRF blocks, enabling future automation of correlative imaging. The ultraLM is a fluorescence microscope that integrates with an ultramicrotome, which enables 'smart collection' of ultrathin sections containing fluorescent cells or tissues for subsequent transmission electron microscopy or array tomography. The miniLM is a fluorescence microscope that integrates with serial block face scanning electron microscopes, which enables 'smart tracking' of fluorescent structures during automated serial electron image acquisition from large cell and tissue volumes.

Cardiac Calcium ATPase Dimerization Measured by Cross-Linking and Fluorescence Energy Transfer.

Science.gov (United States)

Blackwell, Daniel J; Zak, Taylor J; Robia, Seth L

2016-09-20

The cardiac sarco/endoplasmic reticulum calcium ATPase (SERCA) establishes the intracellular calcium gradient across the sarcoplasmic reticulum membrane. It has been proposed that SERCA forms homooligomers that increase the catalytic rate of calcium transport. We investigated SERCA dimerization in rabbit left ventricular myocytes using a photoactivatable cross-linker. Western blotting of cross-linked SERCA revealed higher-molecular-weight species consistent with SERCA oligomerization. Fluorescence resonance energy transfer measurements in cells transiently transfected with fluorescently labeled SERCA2a revealed that SERCA readily forms homodimers. These dimers formed in the absence or presence of the SERCA regulatory partner, phospholamban (PLB) and were unaltered by PLB phosphorylation or changes in calcium or ATP. Fluorescence lifetime data are compatible with a model in which PLB interacts with a SERCA homodimer in a stoichiometry of 1:2. Together, these results suggest that SERCA forms constitutive homodimers in live cells and that dimer formation is not modulated by SERCA conformational poise, PLB binding, or PLB phosphorylation. Copyright © 2016 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Study of the Conformational State of Non-Cross-Linked and Cross-Linked Poly(alkylmethyldiallylammonium chlorides) in Aqueous Solution by Fluorescence Probing

NARCIS (Netherlands)

Wang, Guang-Jia; Engberts, Jan B.F.N.

The aggregation behaviour of novel non-cross-linked and cross-linked poly(alkylmethyldiallylammonium chlorides) in aqueous solutions has been investigated by fluorescence spectroscopy using pyrene as a probe. These copolymers were found to exhibit similar aggregate properties as the corresponding

Feasibility of the simultaneous determination of polycyclic aromatic hydrocarbons based on two-dimensional fluorescence correlation spectroscopy

Science.gov (United States)

Yang, Renjie; Dong, Guimei; Sun, Xueshan; Yang, Yanrong; Yu, Yaping; Liu, Haixue; Zhang, Weiyu

2018-02-01

A new approach for quantitative determination of polycyclic aromatic hydrocarbons (PAHs) in environment was proposed based on two-dimensional (2D) fluorescence correlation spectroscopy in conjunction with multivariate method. 40 mixture solutions of anthracene and pyrene were prepared in the laboratory. Excitation-emission matrix (EEM) fluorescence spectra of all samples were collected. And 2D fluorescence correlation spectra were calculated under the excitation perturbation. The N-way partial least squares (N-PLS) models were developed based on 2D fluorescence correlation spectra, showing a root mean square error of calibration (RMSEC) of 3.50 μg L- 1 and root mean square error of prediction (RMSEP) of 4.42 μg L- 1 for anthracene and of 3.61 μg L- 1 and 4.29 μg L- 1 for pyrene, respectively. Also, the N-PLS models were developed for quantitative analysis of anthracene and pyrene using EEM fluorescence spectra. The RMSEC and RMSEP were 3.97 μg L- 1 and 4.63 μg L- 1 for anthracene, 4.46 μg L- 1 and 4.52 μg L- 1 for pyrene, respectively. It was found that the N-PLS model using 2D fluorescence correlation spectra could provide better results comparing with EEM fluorescence spectra because of its low RMSEC and RMSEP. The methodology proposed has the potential to be an alternative method for detection of PAHs in environment.

Cross-correlation enhanced stability in a tumor cell growth model with immune surveillance driven by cross-correlated noises

International Nuclear Information System (INIS)

Zeng Chunhua; Zhou Xiaofeng; Tao Shufen

2009-01-01

The transient properties of a tumor cell growth model with immune surveillance driven by cross-correlated multiplicative and additive noises are investigated. The explicit expression of extinction rate from the state of a stable tumor to the state of extinction is obtained. Based on the numerical computations, we find the following: (i) the intensity of multiplicative noise D and the intensity of additive noise α enhance the extinction rate for the case of λ ≤ 0 (i.e. λ denotes cross-correlation intensity between two noises), but for the case of λ > 0, a critical noise intensity D or α exists at which the extinction rate is the smallest; D and α at first weaken the extinction rate and then enhance it. (ii) The immune rate β and the cross-correlation intensity λ play opposite roles on the extinction rate, i.e. β enhances the extinction rate of the tumor cell, while λ weakens the extinction rate of the tumor cell. Namely, the immune rate can enhance the extinction of the tumor cell and the cross-correlation between two noises can enhance stability of the cancer state.

Image correlation spectroscopy: mapping correlations in space, time, and reciprocal space.

Science.gov (United States)

Wiseman, Paul W

2013-01-01

This chapter presents an overview of two recent implementations of image correlation spectroscopy (ICS). The background theory is presented for spatiotemporal image correlation spectroscopy and image cross-correlation spectroscopy (STICS and STICCS, respectively) as well as k-(reciprocal) space image correlation spectroscopy (kICS). An introduction to the background theory is followed by sections outlining procedural aspects for properly implementing STICS, STICCS, and kICS. These include microscopy image collection, sampling in space and time, sample and fluorescent probe requirements, signal to noise, and background considerations that are all required to properly implement the ICS methods. Finally, procedural steps for immobile population removal and actual implementation of the ICS analysis programs to fluorescence microscopy image time stacks are described. Copyright © 2013 Elsevier Inc. All rights reserved.

Reorientational motion of a cross-link junction in a poly(dimethylsiloxane) network measured by time-resolved fluorescence depolarization

International Nuclear Information System (INIS)

Stein, A.D.; Hoffman, D.A.; Frank, C.W.; Fayer, M.D.

1992-01-01

The reorientational dynamics of a cross-link junction in poly(dimethylsiloxane) networks, measured by the fluorescence anisotropy decay of a chromophore tagged to the cross-link, have been investigated over a range of temperatures from T g +75 to T g +150. The probe chromophore, 1-dimethylamino-5-sulfonylnaphthalene amide (dansyl amide), is pendant to a trifunctional silane that acts as a cross-linking molecule. In cyclohexanol, the fluorescence anisotropy decay is in agreement with Debye--Stokes--Einstein hydrodynamic theory (rotational diffusion) demonstrating that the cross-linker can be used as a probe of orientational relaxation. The fluorescence anisotropy decays at a rapid rate in an end-linked poly(dimethyl siloxane) network reflecting fast reorientational motion of the cross-link junction. This reorientation appears diffusive and has a temperature dependence in accord with the Williams--Landel--Ferry equation. A model is proposed that suggests that reorientation and translational motion of the cross-link occur simultaneously and are both coupled to fluctuations of the polymer chain ends

Antiphase dual-color correlation in a reactant-product pair imparts ultrasensitivity in reaction-linked double-photoswitching fluorescence imaging.

Science.gov (United States)

Wan, Wei; Zhu, Ming-Qiang; Tian, Zhiyuan; Li, Alexander D Q

2015-04-08

A pair of reversible photochemical reactions correlates their reactant and product specifically, and such a correlation uniquely distinguishes their correlated signal from others that are not linked by this reversible reaction. Here a nanoparticle-shielded fluorophore is photodriven to undergo structural dynamics, alternating between a green-fluorescence state and a red-fluorescence state. As time elapses, the fluorophore can be in either state but not both at the same time. Thus, the red fluorescence is maximized while the green fluorescence is minimized and vice versa. Such an antiphase dual-color (AD) corelationship between the red and green fluorescence maxima as well as between their minima can be exploited to greatly improve the signal-to-noise ratio, thus enhancing the ultimate detection limit. Potential benefits of this correlation include elimination of all interferences originating from single-color dyes and signal amplification of AD photoswitching molecules by orders of magnitude.

Confocal microscopy evaluation of stromal fluorescence intensity after standard and accelerated iontophoresis-assisted corneal cross-linking.

Science.gov (United States)

Lanzini, Manuela; Curcio, Claudia; Spoerl, Eberhard; Calienno, Roberta; Mastropasqua, Alessandra; Colasante, Martina; Mastropasqua, Rodolfo; Nubile, Mario; Mastropasqua, Leonardo

2017-02-01

The aim of this study is to determine modifications in stromal fluorescence intensity after different corneal cross-linking (CXL) procedures and to correlate stromal fluorescence to corneal biomechanical resistance. For confocal microscopy study, 15 human cadaver corneas were examined. Three served as control (group 1), three were just soaked with iontophoresis procedure (group 2), three were treated with standard epi-off technique (group 3), and six underwent iontophoresis imbibition. Three of later six were irradiated for 30 min with 3 mW/cm 2 UVA (group 4) and three for 9 min at 10 mW/cm 2 UVA (group 5). Confocal microscopy was performed to quantify the fluorescence intensity in the cornea at different stromal depths. For biomechanical study, 30 human cadaver corneas were randomly divided into five groups and treated as previously described. Static stress-strain measurements of the corneas were performed. Iontophoresis imbibition followed by 10mW/cm 2 irradiation proved to increase stromal fluorescence into the corneal stroma and significant differences were revealed between group 3 and 5 both at 100 (p = 0.0171) and 250 µm (p = 0.0024), respectively. Biomechanical analysis showed an improvement of corneal resistance in group 5. Iontophoresis imbibition followed by accelerated irradiation increased the stromal fluorescence and is related to an improvement of biomechanical resistance. This approach may represent a new strategy to achieve greater concentrations of riboflavin without removing corneal epithelium and improve clinical results while reducing the side effects of CXL.

Velocity landscape correlation resolves multiple flowing protein populations from fluorescence image time series.

Science.gov (United States)

Pandžić, Elvis; Abu-Arish, Asmahan; Whan, Renee M; Hanrahan, John W; Wiseman, Paul W

2018-02-16

Molecular, vesicular and organellar flows are of fundamental importance for the delivery of nutrients and essential components used in cellular functions such as motility and division. With recent advances in fluorescence/super-resolution microscopy modalities we can resolve the movements of these objects at higher spatio-temporal resolutions and with better sensitivity. Previously, spatio-temporal image correlation spectroscopy has been applied to map molecular flows by correlation analysis of fluorescence fluctuations in image series. However, an underlying assumption of this approach is that the sampled time windows contain one dominant flowing component. Although this was true for most of the cases analyzed earlier, in some situations two or more different flowing populations can be present in the same spatio-temporal window. We introduce an approach, termed velocity landscape correlation (VLC), which detects and extracts multiple flow components present in a sampled image region via an extension of the correlation analysis of fluorescence intensity fluctuations. First we demonstrate theoretically how this approach works, test the performance of the method with a range of computer simulated image series with varying flow dynamics. Finally we apply VLC to study variable fluxing of STIM1 proteins on microtubules connected to the plasma membrane of Cystic Fibrosis Bronchial Epithelial (CFBE) cells. Copyright © 2018 Elsevier Inc. All rights reserved.

Cross-correlation interference effects in multiaccess optical communications

Science.gov (United States)

Peterson, G. D.; Gardner, C. S.

1981-03-01

An analysis is presented of the cross correlation between user codes in an optical code-division multiple-access communication system. The system model is a multiaccess satellite repeater, where the uplink and downlink channels are direct-detection optical-polarization modulation links. The error probability is obtained in terms of the cross correlation between the intended and interfering user codes. It is demonstrated that the system error rate can be minimized by the use of code sequences in which the normalized second moment of the cross correlation between codes is small.

Distribution of diffusion times determined by fluorescence (lifetime) correlation spectroscopy

Czech Academy of Sciences Publication Activity Database

Pánek, Jiří; Loukotová, Lenka; Hrubý, Martin; Štěpánek, Petr

2018-01-01

Roč. 51, č. 8 (2018), s. 2796-2804 ISSN 0024-9297 R&D Projects: GA MŠk(CZ) LO1507 Institutional support: RVO:61389013 Keywords : polymer solution * fluorescence correlation spectroscopy * diffusion time distribution Subject RIV: CD - Macromolecular Chemistry OBOR OECD: Polymer science Impact factor: 5.835, year: 2016

PyCCF: Python Cross Correlation Function for reverberation mapping studies

Science.gov (United States)

Sun, Mouyuan; Grier, C. J.; Peterson, B. M.

2018-05-01

PyCCF emulates a Fortran program written by B. Peterson for use with reverberation mapping. The code cross correlates two light curves that are unevenly sampled using linear interpolation and measures the peak and centroid of the cross-correlation function. In addition, it is possible to run Monto Carlo iterations using flux randomization and random subset selection (RSS) to produce cross-correlation centroid distributions to estimate the uncertainties in the cross correlation results.

Cross-correlations between RMB exchange rate and international commodity markets

Science.gov (United States)

Lu, Xinsheng; Li, Jianfeng; Zhou, Ying; Qian, Yubo

2017-11-01

This paper employs multifractal detrended analysis (MF-DFA) and multifractal detrended cross-correlation analysis (MF-DCCA) to study cross-correlation behaviors between China's RMB exchange rate market and four international commodity markets, using a comprehensive set of data covering the period from 22 July 2005 to 15 March 2016. Our empirical results from MF-DFA indicate that the RMB exchange rate is the most inefficient among the 4 selected markets. The results from quantitative analysis have testified the existence of cross-correlations and the result from MF-DCCA have further confirmed a strong multifractal behavior between RMB exchange rate and international commodity markets. We also demonstrate that the recent financial crisis has significant impact on the cross-correlated behavior. Through the rolling window analysis, we find that the RMB exchange rates and international commodity prices are anti-persistent cross-correlated. The main sources of multifractality in the cross-correlations are long-range correlations between RMB exchange rate and the aggregate commodity, energy and metals index.

Probing GFP-actin diffusion in living cells using fluorescence correlation spectroscopy

International Nuclear Information System (INIS)

Engelke, Hanna; Heinrich, Doris; Rädler, Joachim O.

2010-01-01

The cytoskeleton of eukaryotic cells is continuously remodeled by polymerization and depolymerization of actin. Consequently, the relative content of polymerized filamentous actin (F-actin) and monomeric globular actin (G-actin) is subject to temporal and spatial fluctuations. Since fluorescence correlation spectroscopy (FCS) can measure the diffusion of fluorescently labeled actin it seems likely that FCS allows us to determine the dynamics and hence indirectly the structural properties of the cytoskeleton components with high spatial resolution. To this end we investigate the FCS signal of GFP-actin in living Dictyostelium discoideum cells and explore the inherent spatial and temporal signatures of the actin cytoskeleton. Using the free green fluorescent protein (GFP) as a reference, we find that actin diffusion inside cells is dominated by G-actin and slower than diffusion in diluted cell extract. The FCS signal in the dense cortical F-actin network near the cell membrane is probed using the cytoskeleton protein LIM and is found to be slower than cytosolic G-actin diffusion. Furthermore, we show that polymerization of the cytoskeleton induced by Jasplakinolide leads to a substantial decrease of G-actin diffusion. Pronounced fluctuations in the distribution of the FCS correlation curves can be induced by latrunculin, which is known to induce actin waves. Our work suggests that the FCS signal of GFP-actin in combination with scanning or spatial correlation techniques yield valuable information about the local dynamics and concomitant cytoskeletal properties

Reorientational motion of a cross-link junction in a poly(dimethylsiloxane) network measured by time-resolved fluorescence depolarization

Energy Technology Data Exchange (ETDEWEB)

Stein, A.D. (Department of Chemistry, Stanford University, Stanford, California 94305 (United States)); Hoffman, D.A. (Department of Materials Science and Engineering, Stanford University, Stanford, California 94305 (United States)); Frank, C.W. (Department of Chemical Engineering, Stanford University, Stanford, California 94305 (United States)); Fayer, M.D. (Department of Chemistry, Stanford University, Stanford, California 94305 (United States))

1992-02-15

The reorientational dynamics of a cross-link junction in poly(dimethylsiloxane) networks, measured by the fluorescence anisotropy decay of a chromophore tagged to the cross-link, have been investigated over a range of temperatures from {ital T}{sub {ital g}}+75 to {ital T}{sub {ital g}}+150. The probe chromophore, 1-dimethylamino-5-sulfonylnaphthalene amide (dansyl amide), is pendant to a trifunctional silane that acts as a cross-linking molecule. In cyclohexanol, the fluorescence anisotropy decay is in agreement with Debye--Stokes--Einstein hydrodynamic theory (rotational diffusion) demonstrating that the cross-linker can be used as a probe of orientational relaxation. The fluorescence anisotropy decays at a rapid rate in an end-linked poly(dimethyl siloxane) network reflecting fast reorientational motion of the cross-link junction. This reorientation appears diffusive and has a temperature dependence in accord with the Williams--Landel--Ferry equation. A model is proposed that suggests that reorientation and translational motion of the cross-link occur simultaneously and are both coupled to fluctuations of the polymer chain ends.

Measurements of excited-state-to-excited-state transition probabilities and photoionization cross-sections using laser-induced fluorescence and photoionization signals

International Nuclear Information System (INIS)

Shah, M.L.; Sahoo, A.C.; Pulhani, A.K.; Gupta, G.P.; Dikshit, B.; Bhatia, M.S.; Suri, B.M.

2014-01-01

Laser-induced photoionization and fluorescence signals were simultaneously observed in atomic samarium using Nd:YAG-pumped dye lasers. Two-color, three-photon photoionization and two-color fluorescence signals were recorded simultaneously as a function of the second-step laser power for two photoionization pathways. The density matrix formalism has been employed to analyze these signals. Two-color laser-induced fluorescence signal depends on the laser powers used for the first and second-step transitions as well as the first and second-step transition probability whereas two-color, three-photon photoionization signal depends on the third-step transition cross-section at the second-step laser wavelength along with the laser powers and transition probability for the first and second-step transitions. Two-color laser-induced fluorescence was used to measure the second-step transition probability. The second-step transition probability obtained was used to infer the photoionization cross-section. Thus, the methodology combining two-color, three-photon photoionization and two-color fluorescence signals in a single experiment has been established for the first time to measure the second-step transition probability as well as the photoionization cross-section. - Highlights: • Laser-induced photoionization and fluorescence signals have been simultaneously observed. • The density matrix formalism has been employed to analyze these signals. • Two-color laser-induced fluorescence was used to measure the second-step transition probability. • The second-step transition probability obtained was used to infer the photoionization cross-section. • Transition probability and photoionization cross-section have been measured in a single experiment

Alloying effect on K shell X-ray fluorescence cross-sections and yields in Ti-Ni based shape memory alloys

Directory of Open Access Journals (Sweden)

Bünyamin Alım

2018-04-01

Full Text Available K shell X-ray fluorescence cross-sections (σKα, σKβ and σK, and K shell fluorescence yields (ωK of Ti, Ni both in pure metals and in different alloy compositions (TixNi1-x; x = 0.3, 0.4, 0.5, 0.6, 0.7 were measured by using energy dispersive X-ray fluorescence (EDXRF technique. The samples were excited by 22.69 keV X-rays from a 10 mCi Cd-109 radioactive point source and K X rays emitted by samples were counted by a high resolution Si(Li solid-state detector coupled to a 4 K multichannel analyzer (MCA. The alloying effects on the X-ray fluorescence (XRF parameters of Ti-Ni shape memory alloys (SMAs were investigated. It is clearly observed that alloying effect causes to change in K shell XRF parameter values in Ti-Ni based SMAs for different compositions of x. Also, the present investigation makes it possible to perform reliable interpretation of experimental σKα, σKβ and ωK values for Ti and Ni in SMAs and can also provide quantitative information about the changes of K shell X-ray fluorescence cross sections and fluorescence yields of these metals with alloy composition. Keywords: Alloying effect, XRF, K X-ray fluorescence cross-section, K shell fluorescence yield, Shape memory alloy

Strong anticipation and long-range cross-correlation: Application of detrended cross-correlation analysis to human behavioral data

Science.gov (United States)

Delignières, Didier; Marmelat, Vivien

2014-01-01

In this paper, we analyze empirical data, accounting for coordination processes between complex systems (bimanual coordination, interpersonal coordination, and synchronization with a fractal metronome), by using a recently proposed method: detrended cross-correlation analysis (DCCA). This work is motivated by the strong anticipation hypothesis, which supposes that coordination between complex systems is not achieved on the basis of local adaptations (i.e., correction, predictions), but results from a more global matching of complexity properties. Indeed, recent experiments have evidenced a very close correlation between the scaling properties of the series produced by two coordinated systems, despite a quite weak local synchronization. We hypothesized that strong anticipation should result in the presence of long-range cross-correlations between the series produced by the two systems. Results allow a detailed analysis of the effects of coordination on the fluctuations of the series produced by the two systems. In the long term, series tend to present similar scaling properties, with clear evidence of long-range cross-correlation. Short-term results strongly depend on the nature of the task. Simulation studies allow disentangling the respective effects of noise and short-term coupling processes on DCCA results, and suggest that the matching of long-term fluctuations could be the result of short-term coupling processes.

CROSS-CORRELATIONS AS A COSMOLOGICAL CARBON MONOXIDE DETECTOR

International Nuclear Information System (INIS)

Pullen, Anthony R.; Doré, Olivier; Chang, Tzu-Ching; Lidz, Adam

2013-01-01

We present a new procedure to measure the large-scale carbon monoxide (CO) emissions across cosmic history. As a tracer of large-scale structure (LSS), the CO gas content as a function of redshift can be quantified by its three-dimensional fluctuation power spectra. Furthermore, cross-correlating CO emission with other LSS tracers offers a way to measure the emission as a function of scale and redshift. Here we introduce the model relevant for such a cross-correlation measurement between CO and other LSS tracers, and between different CO rotational lines. We propose a novel use of cosmic microwave background (CMB) data and attempt to extract redshifted CO emissions embedded in the Wilkinson Microwave Anisotropy Probe (WMAP) data set. We cross-correlate the all-sky WMAP7 data with LSS data sets, namely, the photometric quasar sample and the luminous red galaxy sample from the Sloan Digital Sky Survey Data Releases 6 and 7, respectively. We are unable to detect a cross-correlation signal with either CO(1-0) or CO(2-1) lines, mainly due to the instrumental noise in the WMAP data. However, we are able to rule out models more than three times greater than our more optimistic model. We discuss the cross-correlation signal from the thermal Sunyaev-Zeldovich effect and dust as potential contaminants, and quantify their impact for our CO measurements. We discuss forecasts for current CMB experiments and a hypothetical future CO-focused experiment, and propose to cross-correlate CO temperature data with the Hobby-Eberly Telescope Dark Energy Experiment Lyα-emitter sample, for which a signal-to-noise ratio of 58 is possible.

Cross-correlation time-of-flight analysis of molecular beam scattering

International Nuclear Information System (INIS)

Nowikow, C.V.; Grice, R.

1979-01-01

The theory of the cross-correlation method of time-of-flight analysis is presented in a form which highlights its formal similarity to the conventional method. A time-of-flight system for the analysis of crossed molecular beam scattering is described, which is based on a minicomputer interface and can operate in both the cross-correlation and conventional modes. The interface maintains the synchronisation of chopper disc rotation and channel advance indefinitely in the cross-correlation method and can acquire data in phase with the beam modulation in both methods. The shutter function of the cross-correlation method is determined and the deconvolution analysis of the data is discussed. (author)

Simultaneous membrane interaction of amphipathic peptide monomers, self-aggregates and cargo complexes detected by fluorescence correlation spectroscopy.

Science.gov (United States)

Vasconcelos, Luís; Lehto, Tõnis; Madani, Fatemeh; Radoi, Vlad; Hällbrink, Mattias; Vukojević, Vladana; Langel, Ülo

2018-02-01

Peptides able to translocate cell membranes while carrying macromolecular cargo, as cell-penetrating peptides (CPPs), can contribute to the field of drug delivery by enabling the transport of otherwise membrane impermeable molecules. Formation of non-covalent complexes between amphipathic peptides and oligonucleotides is driven by electrostatic and hydrophobic interactions. Here we investigate and quantify the coexistence of distinct molecular species in multiple equilibria, namely peptide monomer, peptide self-aggregates and peptide/oligonucleotide complexes. As a model for the complexes, we used a stearylated peptide from the PepFect family, PF14 and siRNA. PF14 has a cationic part and a lipid part, resembling some characteristics of cationic lipids. Fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) were used to detect distinct molecular entities in solution and at the plasma membrane of live cells. For that, we labeled the peptide with carboxyrhodamine 6G and the siRNA with Cyanine 5. We were able to detect fluorescent entities with diffusional properties characteristic of the peptide monomer as well as of peptide aggregates and peptide/oligonucleotide complexes. Strategies to avoid peptide adsorption to solid surfaces and self-aggregation were developed and allowed successful FCS measurements in solution and at the plasma membrane. The ratio between the detected molecular species was found to vary with pH, peptide concentration and the proximity to the plasma membrane. The present results suggest that the diverse cellular uptake mechanisms, often reported for amphipathic CPPs, might result from the synergistic effect of peptide monomers, self-aggregates and cargo complexes, distributed unevenly at the plasma membrane. Copyright © 2017 Elsevier B.V. All rights reserved.

Detecting PM2.5's Correlations between Neighboring Cities Using a Time-Lagged Cross-Correlation Coefficient.

Science.gov (United States)

Wang, Fang; Wang, Lin; Chen, Yuming

2017-08-31

In order to investigate the time-dependent cross-correlations of fine particulate (PM2.5) series among neighboring cities in Northern China, in this paper, we propose a new cross-correlation coefficient, the time-lagged q-L dependent height crosscorrelation coefficient (denoted by p q (τ, L)), which incorporates the time-lag factor and the fluctuation amplitude information into the analogous height cross-correlation analysis coefficient. Numerical tests are performed to illustrate that the newly proposed coefficient ρ q (τ, L) can be used to detect cross-correlations between two series with time lags and to identify different range of fluctuations at which two series possess cross-correlations. Applying the new coefficient to analyze the time-dependent cross-correlations of PM2.5 series between Beijing and the three neighboring cities of Tianjin, Zhangjiakou, and Baoding, we find that time lags between the PM2.5 series with larger fluctuations are longer than those between PM2.5 series withsmaller fluctuations. Our analysis also shows that cross-correlations between the PM2.5 series of two neighboring cities are significant and the time lags between two PM2.5 series of neighboring cities are significantly non-zero. These findings providenew scientific support on the view that air pollution in neighboring cities can affect one another not simultaneously but with a time lag.

Cross-correlations between agricultural commodity futures markets in the US and China

Science.gov (United States)

Li, Zhihui; Lu, Xinsheng

2012-08-01

This paper examines the cross-correlation properties of agricultural futures markets between the US and China using a cross-correlation statistic test and multifractal detrended cross-correlation analysis (MF-DCCA). The results show that the cross-correlations between the two geographically distant markets for four pairs of important agricultural commodities futures are significantly multifractal. By introducing the concept of a “crossover”, we find that the multifractality of cross-correlations between the two markets is not long lasting. The cross-correlations in the short term are more strongly multifractal, but they are weakly so in the long term. Moreover, cross-correlations of small fluctuations are persistent and those of large fluctuations are anti-persistent in the short term while cross-correlations of all kinds of fluctuations for soy bean and soy meal futures are persistent and for corn and wheat futures are anti-persistent in the long term. We also find that cross-correlation exponents are less than the averaged generalized Hurst exponent when q0 in the short term, while in the long term they are almost the same.

Sizes of water-soluble luminescent quantum dots measured by fluorescence correlation spectroscopy

International Nuclear Information System (INIS)

Zhang Pudun; Li Liang; Dong Chaoqing; Qian Huifeng; Ren Jicun

2005-01-01

In this paper, fluorescence correlation spectroscopy (FCS) was applied to measure the size of water-soluble quantum dots (QDs). The measurements were performed on a home-built FCS system based on the Stokes-Einstein equation. The obtained results showed that for bare CdTe QDs the sizes from FCS were larger than the ones from transmission electron microscopy (TEM). The brightness of QDs was also evaluated using FCS technique. It was found that the stability of the surface chemistry of QDs would be significantly improved by capping it with hard-core shell. Our data demonstrated that FCS is a simple, fast, and effective method for characterizing the fluorescent quantum dots, and is especially suitable for determining the fluorescent nanoparticles less than 10 nm in water solution

Complementary studies of lipid membrane dynamics using iSCAT and super-resolved fluorescence correlation spectroscopy

Science.gov (United States)

Reina, Francesco; Galiani, Silvia; Shrestha, Dilip; Sezgin, Erdinc; de Wit, Gabrielle; Cole, Daniel; Lagerholm, B. Christoffer; Kukura, Philipp; Eggeling, Christian

2018-06-01

Observation techniques with high spatial and temporal resolution, such as single-particle tracking based on interferometric scattering (iSCAT) microscopy, and fluorescence correlation spectroscopy applied on a super-resolution STED microscope (STED-FCS), have revealed new insights of the molecular organization of membranes. While delivering complementary information, there are still distinct differences between these techniques, most prominently the use of fluorescent dye tagged probes for STED-FCS and a need for larger scattering gold nanoparticle tags for iSCAT. In this work, we have used lipid analogues tagged with a hybrid fluorescent tag–gold nanoparticle construct, to directly compare the results from STED-FCS and iSCAT measurements of phospholipid diffusion on a homogeneous supported lipid bilayer (SLB). These comparative measurements showed that while the mode of diffusion remained free, at least at the spatial (>40 nm) and temporal (50  ⩽  t  ⩽  100 ms) scales probed, the diffussion coefficient was reduced by 20- to 60-fold when tagging with 20 and 40 nm large gold particles as compared to when using dye tagged lipid analogues. These FCS measurements of hybrid fluorescent tag–gold nanoparticle labeled lipids also revealed that commercially supplied streptavidin-coated gold nanoparticles contain large quantities of free streptavidin. Finally, the values of apparent diffusion coefficients obtained by STED-FCS and iSCAT differed by a factor of 2–3 across the techniques, while relative differences in mobility between different species of lipid analogues considered were identical in both approaches. In conclusion, our experiments reveal that large and potentially cross-linking scattering tags introduce a significant slow-down in diffusion on SLBs but no additional bias, and our labeling approach creates a new way of exploiting complementary information from STED-FCS and iSCAT measurements.

Speckle correlation resolution enhancement of wide-field fluorescence imaging (Conference Presentation)

Science.gov (United States)

Yilmaz, Hasan

2016-03-01

Structured illumination enables high-resolution fluorescence imaging of nanostructures [1]. We demonstrate a new high-resolution fluorescence imaging method that uses a scattering layer with a high-index substrate as a solid immersion lens [2]. Random scattering of coherent light enables a speckle pattern with a very fine structure that illuminates the fluorescent nanospheres on the back surface of the high-index substrate. The speckle pattern is raster-scanned over the fluorescent nanospheres using a speckle correlation effect known as the optical memory effect. A series of standard-resolution fluorescence images per each speckle pattern displacement are recorded by an electron-multiplying CCD camera using a commercial microscope objective. We have developed a new phase-retrieval algorithm to reconstruct a high-resolution, wide-field image from several standard-resolution wide-field images. We have introduced phase information of Fourier components of standard-resolution images as a new constraint in our algorithm which discards ambiguities therefore ensures convergence to a unique solution. We demonstrate two-dimensional fluorescence images of a collection of nanospheres with a deconvolved Abbe resolution of 116 nm and a field of view of 10 µm × 10 µm. Our method is robust against optical aberrations and stage drifts, therefore excellent for imaging nanostructures under ambient conditions. [1] M. G. L. Gustafsson, J. Microsc. 198, 82-87 (2000). [2] H. Yilmaz, E. G. van Putten, J. Bertolotti, A. Lagendijk, W. L. Vos, and A. P. Mosk, Optica 2, 424-429 (2015).

Cross-correlations between Renminbi and four major currencies in the Renminbi currency basket

Science.gov (United States)

Wang, Gang-Jin; Xie, Chi

2013-03-01

We investigate the cross-correlations between Renminbi (CNY) and four major currencies (USD, EUR, JPY, and KRW) in the Renminbi currency basket, i.e., the cross-correlations of CNY-USD, CNY-EUR, CNY-JPY, and CNY-KRW. Qualitatively, using a statistical test in analogy to the Ljung-Box test, we find that cross-correlations significantly exist in CNY-USD, CNY-EUR, CNY-JPY, and CNY-KRW. Quantitatively, employing the detrended cross-correlation analysis (DCCA) method, we find that the cross-correlations of CNY-USD, CNY-EUR, CNY-JPY, and CNY-KRW are weakly persistent. We use the DCCA cross-correlation coefficient ρ to quantify the level of cross-correlations and find the currency weight in the Renminbi currency basket is arranged in the order of USD>EUR>JPY >KRW. Using the method of rolling windows, which can capture the time-varying cross-correlation scaling exponents, we find that: (i) CNY and USD are positively cross-correlated over time, but the cross-correlations of CNY-USD are anti-persistent during the US sub-prime crisis and the European debt crisis. (ii) The cross-correlation scaling exponents of CNY-EUR have the cyclical fluctuation with a nearly two-year cycle. (iii) CNY-JPY has long-term negative cross-correlations, during the European debt crisis, but CNY and KRW are positively cross-correlated.

Colocalization analysis in fluorescence micrographs: verification of a more accurate calculation of pearson's correlation coefficient.

Science.gov (United States)

Barlow, Andrew L; Macleod, Alasdair; Noppen, Samuel; Sanderson, Jeremy; Guérin, Christopher J

2010-12-01

One of the most routine uses of fluorescence microscopy is colocalization, i.e., the demonstration of a relationship between pairs of biological molecules. Frequently this is presented simplistically by the use of overlays of red and green images, with areas of yellow indicating colocalization of the molecules. Colocalization data are rarely quantified and can be misleading. Our results from both synthetic and biological datasets demonstrate that the generation of Pearson's correlation coefficient between pairs of images can overestimate positive correlation and fail to demonstrate negative correlation. We have demonstrated that the calculation of a thresholded Pearson's correlation coefficient using only intensity values over a determined threshold in both channels produces numerical values that more accurately describe both synthetic datasets and biological examples. Its use will bring clarity and accuracy to colocalization studies using fluorescent microscopy.

Detection of rheumatoid arthritis by evaluation of normalized variances of fluorescence time correlation functions

Science.gov (United States)

Dziekan, Thomas; Weissbach, Carmen; Voigt, Jan; Ebert, Bernd; MacDonald, Rainer; Bahner, Malte L.; Mahler, Marianne; Schirner, Michael; Berliner, Michael; Berliner, Birgitt; Osel, Jens; Osel, Ilka

2011-07-01

Fluorescence imaging using the dye indocyanine green as a contrast agent was investigated in a prospective clinical study for the detection of rheumatoid arthritis. Normalized variances of correlated time series of fluorescence intensities describing the bolus kinetics of the contrast agent in certain regions of interest were analyzed to differentiate healthy from inflamed finger joints. These values are determined using a robust, parameter-free algorithm. We found that the normalized variance of correlation functions improves the differentiation between healthy joints of volunteers and joints with rheumatoid arthritis of patients by about 10% compared to, e.g., ratios of areas under the curves of raw data.

Testing power-law cross-correlations: Rescaled covariance test

Czech Academy of Sciences Publication Activity Database

Krištoufek, Ladislav

2013-01-01

Roč. 86, č. 10 (2013), 418-1-418-15 ISSN 1434-6028 R&D Projects: GA ČR GA402/09/0965 Institutional support: RVO:67985556 Keywords : power-law cross-correlations * testing * long-term memory Subject RIV: AH - Economics Impact factor: 1.463, year: 2013 http://library.utia.cas.cz/separaty/2013/E/kristoufek-testing power-law cross-correlations rescaled covariance test.pdf

The Pierre Auger fluorescence detector. Cross-checking the absolute calibration using a drone

Energy Technology Data Exchange (ETDEWEB)

Tomankova, Lenka [Institute for Nuclear Physics (IKP), Karlsruhe Institute of Technology (KIT), 76021 Karlsruhe (Germany); Collaboration: Pierre-Auger-Collaboration

2016-07-01

The Pierre Auger Observatory combines the air shower fluorescence and surface array methods to study ultra-high energy cosmic rays. As the energy scale of the experiment is derived from calorimetric measurements by the fluorescence telescopes, their accurate calibration is of primary importance to all Auger data. We discuss a novel calibration method based on a remotely flown drone equipped with a specially designed light source that mimics a snapshot of an air shower traversing the atmosphere. Several drone measurement campaigns have been performed to study the properties of the Auger fluorescence telescopes and to derive an end-to-end calibration. We give an overview of the measurements and present the basic analysis chain as well as the first results of an independent cross-check of the Auger energy scale.

A library for X-ray-matter interaction cross sections for X-ray fluorescence applications

Energy Technology Data Exchange (ETDEWEB)

Brunetti, A. [Istituto di Matematica e Fisica, Universita di Sassari, via Vienna 2, 07100 Sassari (Italy) and INFN, Sezione di Cagliari (Italy)]. E-mail: brunetti@uniss.it; Sanchez del Rio, M. [European Synchrotron Radiation Facility, 6 rue Jules Horowitz, 38043 Grenoble Cedex (France); Golosio, B. [INFN, Sezione di Cagliari (Italy); European Synchrotron Radiation Facility, 6 rue Jules Horowitz, 38043 Grenoble Cedex (France); Simionovici, A. [European Synchrotron Radiation Facility, 6 rue Jules Horowitz, 38043 Grenoble Cedex (France); Laboratoire de Sciences de la Terre, Ecole Normale Superieure, Lyon, F-69364 (France); Somogyi, A. [European Synchrotron Radiation Facility, 6 rue Jules Horowitz, 38043 Grenoble Cedex (France)

2004-10-08

Quantitative estimate of elemental composition by spectroscopic and imaging techniques using X-ray fluorescence requires the availability of accurate data of X-ray interaction with matter. Although a wide number of computer codes and data sets are reported in literature, none of them is presented in the form of freely available library functions which can be easily included in software applications for X-ray fluorescence. This work presents a compilation of data sets from different published works and an xraylib interface in the form of callable functions. Although the target applications are on X-ray fluorescence, cross sections of interactions like photoionization, coherent scattering and Compton scattering, as well as form factors and anomalous scattering functions, are also available.

Cross-correlations between crude oil and exchange markets for selected oil rich economies

Science.gov (United States)

Li, Jianfeng; Lu, Xinsheng; Zhou, Ying

2016-07-01

Using multifractal detrended cross-correlation analysis (MF-DCCA), this paper studies the cross-correlation behavior between crude oil market and five selected exchange rate markets. The dataset covers the period of January 1,1996-December 31,2014, and contains 4,633 observations for each of the series, including daily closing prices of crude oil, Australian Dollars, Canadian Dollars, Mexican Pesos, Russian Rubles, and South African Rand. Our empirical results obtained from cross-correlation statistic and cross-correlation coefficient have confirmed the existence of cross-correlations, and the MF-DCCA results have demonstrated a strong multifractality between cross-correlated crude oil market and exchange rate markets in both short term and long term. Using rolling window analysis, we have also found the persistent cross-correlations between the exchange rates and crude oil returns, and the cross-correlation scaling exponents exhibit volatility during some time periods due to its sensitivity to sudden events.

Fluorescence decay time imaging using an imaging photon detector with a radio frequency photon correlation system

Science.gov (United States)

Morgan, Christopher G.; Mitchell, A. C.; Murray, J. G.

1990-05-01

An imaging photon detector has been modified to incorporate fast timing electronics coupled to a custom built photon correlator interfaced to a RISC computer. Using excitation with intensity- muodulated light, fluorescence images can be readily obtained where contrast is determined by the decay time of emission, rather than by intensity. This technology is readily extended to multifrequency phase/demodulation fluorescence imaging or to differential polarised phase fluorometry. The potential use of the correlator for confocal imaging with a laser scanner is also briefly discussed.

General renormalized statistical approach with finite cross-field correlations

International Nuclear Information System (INIS)

Vakulenko, M.O.

1992-01-01

The renormalized statistical approach is proposed, accounting for finite correlations of potential and magnetic fluctuations. It may be used for analysis of a wide class of nonlinear model equations describing the cross-correlated plasma states. The influence of a cross spectrum on stationary potential and magnetic ones is investigated. 10 refs. (author)

Current cross-correlations in double quantum dot Cooper pair splitter

Energy Technology Data Exchange (ETDEWEB)

Wrzesniewski, Kacper; Trocha, Piotr; Weymann, Ireneusz [Faculty of Physics, Adam Mickiewicz University, 61-614 Poznan (Poland)

2016-07-01

We investigate theoretically transport properties of a quantum dot (QD) system working as a Cooper pair splitter. The device is coupled to one superconducting and two ferromagnetic leads. Presented results are calculated using real-time diagrammatic technique in the sequential tunneling approximation with respect to the coupling to ferromagnetic leads. The transport properties are evaluated within the superconductor subgap regime taking into account Andreev reflection processes solely. We focus on the analysis of current and current cross-correlations, both in linear and nonlinear responses. Current cross-correlations give additional information about dynamics of transport processes. We identify both positive and negative signs of current cross-correlations and discuss mechanisms leading to those results. Strong negative cross-correlations are found when the occupation number of QD system becomes degenerate and near the emergence of the triplet blockade, while positive ones occur in the most range where current flows due to crossed Andreev processes. Finally, we consider ferromagnetic leads polarization and temperature influences on aforementioned features.

Cross-Linked Fluorescent Supramolecular Nanoparticles as Finite Tattoo Pigments with Controllable Intradermal Retention Times.

Science.gov (United States)

Choi, Jin-Sil; Zhu, Yazhen; Li, Hongsheng; Peyda, Parham; Nguyen, Thuy Tien; Shen, Mo Yuan; Yang, Yang Michael; Zhu, Jingyi; Liu, Mei; Lee, Mandy M; Sun, Shih-Sheng; Yang, Yang; Yu, Hsiao-Hua; Chen, Kai; Chuang, Gary S; Tseng, Hsian-Rong

2017-01-24

Tattooing has been utilized by the medical community for precisely demarcating anatomic landmarks. This practice is especially important for identifying biopsy sites of nonmelanoma skin cancer (NMSC) due to the long interval (i.e., up to 3 months) between the initial diagnostic biopsy and surgical treatment. Commercially available tattoo pigments possess several issues, which include causing poor cosmesis, being mistaken for a melanocytic lesion, requiring additional removal procedures when no longer desired, and potentially inducing inflammatory responses. The ideal tattoo pigment for labeling of skin biopsy sites for NMSC requires (i) invisibility under ambient light, (ii) fluorescence under a selective light source, (iii) a finite intradermal retention time (ca. 3 months), and (iv) biocompatibility. Herein, we introduce cross-linked fluorescent supramolecular nanoparticles (c-FSNPs) as a "finite tattoo" pigment, with optimized photophysical properties and intradermal retention time to achieve successful in vivo finite tattooing. Fluorescent supramolecular nanoparticles encapsulate a fluorescent conjugated polymer, poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenevinylene] (MPS-PPV), into a core via a supramolecular synthetic approach. FSNPs which possess fluorescent properties superior to those of the free MPS-PPV are obtained through a combinatorial screening process. Covalent cross-linking of FSNPs results in micrometer-sized c-FSNPs, which exhibit a size-dependent intradermal retention. The 1456 nm sized c-FSNPs display an ideal intradermal retention time (ca. 3 months) for NMSC lesion labeling, as observed in an in vivo tattoo study. In addition, the c-FSNPs induce undetectable inflammatory responses after tattooing. We believe that the c-FSNPs can serve as a "finite tattoo" pigment to label potential malignant NMSC lesions.

Angular dependence of Ll, L $\\alpha$ , L $\\beta$ and L $\\gamma$ X-ray differential and fluorescence cross-sections for Er, Ta, W, Au, Hg and Tl

CERN Document Server

Demir, L; Kurucu, Y; Karabulut, A; Sahin, Y; 10.1016/S0969-806X(02)00501-7

2003-01-01

Ll, L alpha , L beta and L gamma X-ray differential cross-sections, fluorescence cross-sections and L/sub i/-subshell ( sigma /sub L1/, sigma /sub L2/, and sigma /sub L3/) fluorescence cross-sections were measured for Er, Ta, W, Au, Hg, and Tl at an excitation energy of 59.6 keV using a Si(Li) detector. The differential cross-sections for these elements have been measured at different angles varying from 54 degrees to 153 degrees at intervals of 9 degrees . The Ll and L alpha groups in the L X-ray lines are found to be spatially anisotropic, while those in the L beta and L gamma peaks are isotropic. Experimental and theoretical values of L X-ray fluorescence cross- sections and L/sub i/-subshell X-ray fluorescence cross-sections were compared. (20 refs).

Stationary-phase integrals in the cross correlation of ambient noise

NARCIS (Netherlands)

Boschi, L.; Weemstra, C.

2015-01-01

The cross correlation of ambient signal allows seismologists to collect data even in the absence of seismic events. “Seismic interferometry” shows that the cross correlation of simultaneous recordings of a random wavefield made at two locations is formally related to the impulse response between

Thermally activated delayed fluorescence of fluorescein derivative for time-resolved and confocal fluorescence imaging.

Science.gov (United States)

Xiong, Xiaoqing; Song, Fengling; Wang, Jingyun; Zhang, Yukang; Xue, Yingying; Sun, Liangliang; Jiang, Na; Gao, Pan; Tian, Lu; Peng, Xiaojun

2014-07-09

Compared with fluorescence imaging utilizing fluorophores whose lifetimes are in the order of nanoseconds, time-resolved fluorescence microscopy has more advantages in monitoring target fluorescence. In this work, compound DCF-MPYM, which is based on a fluorescein derivative, showed long-lived luminescence (22.11 μs in deaerated ethanol) and was used in time-resolved fluorescence imaging in living cells. Both nanosecond time-resolved transient difference absorption spectra and time-correlated single-photon counting (TCSPC) were employed to explain the long lifetime of the compound, which is rare in pure organic fluorophores without rare earth metals and heavy atoms. A mechanism of thermally activated delayed fluorescence (TADF) that considers the long wavelength fluorescence, large Stokes shift, and long-lived triplet state of DCF-MPYM was proposed. The energy gap (ΔEST) of DCF-MPYM between the singlet and triplet state was determined to be 28.36 meV by the decay rate of DF as a function of temperature. The ΔE(ST) was small enough to allow efficient intersystem crossing (ISC) and reverse ISC, leading to efficient TADF at room temperature. The straightforward synthesis of DCF-MPYM and wide availability of its starting materials contribute to the excellent potential of the compound to replace luminescent lanthanide complexes in future time-resolved imaging technologies.

Statistical properties of cross-correlation in the Korean stock market

Science.gov (United States)

Oh, G.; Eom, C.; Wang, F.; Jung, W.-S.; Stanley, H. E.; Kim, S.

2011-01-01

We investigate the statistical properties of the cross-correlation matrix between individual stocks traded in the Korean stock market using the random matrix theory (RMT) and observe how these affect the portfolio weights in the Markowitz portfolio theory. We find that the distribution of the cross-correlation matrix is positively skewed and changes over time. We find that the eigenvalue distribution of original cross-correlation matrix deviates from the eigenvalues predicted by the RMT, and the largest eigenvalue is 52 times larger than the maximum value among the eigenvalues predicted by the RMT. The β_{473} coefficient, which reflect the largest eigenvalue property, is 0.8, while one of the eigenvalues in the RMT is approximately zero. Notably, we show that the entropy function E(σ) with the portfolio risk σ for the original and filtered cross-correlation matrices are consistent with a power-law function, E( σ) σ^{-γ}, with the exponent γ 2.92 and those for Asian currency crisis decreases significantly.

Image processing by use of the digital cross-correlator

International Nuclear Information System (INIS)

Katou, Yoshinori

1982-01-01

We manufactured for trial an instrument which achieved the image processing using digital correlators. A digital correlator perform 64-bit parallel correlation at 20 MH. The output of a digital correlator is a 7-bit word representing. An A-D converter is used to quantize it a precision of six bits. The resulting 6-bit word is fed to six correlators, wired in parallel. The image processing achieved in 12 bits, whose digital outputs converted an analog signal by a D-A converter. This instrument is named the digital cross-correlator. The method which was used in the image processing system calculated the convolution with the digital correlator. It makes various digital filters. In the experiment with the image processing video signals from TV camera were used. The digital image processing time was approximately 5 μs. The contrast was enhanced and smoothed. The digital cross-correlator has the image processing of 16 sorts, and was produced inexpensively. (author)

Detrended cross-correlation analysis on RMB exchange rate and Hang Seng China Enterprises Index

Science.gov (United States)

Ruan, Qingsong; Yang, Bingchan; Ma, Guofeng

2017-02-01

In this paper, we investigate the cross-correlations between the Hang Seng China Enterprises Index and RMB exchange markets on the basis of a cross-correlation statistic test and multifractal detrended cross-correlation analysis (MF-DCCA). MF-DCCA has, at best, serious limitations for most of the signals describing complex natural processes and often indicates multifractal cross-correlations when there are none. In order to prevent these false multifractal cross-correlations, we apply MFCCA to verify the cross-correlations. Qualitatively, we find that the return series of the Hang Seng China Enterprises Index and RMB exchange markets were, overall, significantly cross-correlated based on the statistical analysis. Quantitatively, we find that the cross-correlations between the stock index and RMB exchange markets were strongly multifractal, and the multifractal degree of the onshore RMB exchange markets was somewhat larger than the offshore RMB exchange markets. Moreover, we use the absolute return series to investigate and confirm the fact of multifractality. The results from the rolling windows show that the short-term cross-correlations between volatility series remain high.

Cross-correlation analysis of Ge/Li/ spectra

International Nuclear Information System (INIS)

MacDonald, R.; Robertson, A.; Kennett, T.J.; Prestwich, W.V.

1974-01-01

A sensitive technique is proposed for activation analysis using cross-correlation and improved spectral orthogonality achieved through use of a rectangular zero area digital filter. To test the accuracy and reliability of the cross-correlation procedure five spectra obtained with a Ge/Li detector were combined in different proportions. Gaussian distributed statistics were then added to the composite spectra by means of a pseudo-random number generator. The basis spectra used were 76 As, 82 Br, 72 Ga, 77 Ge, and room background. In general, when the basis spectra were combined in roughly comparable proportions the accuracy of the techique proved to be excelent (>1%). However, of primary importance was the ability of the correlation technique to identify low intensity components in the presence of high intensity components. It was found that the detection threshold for Ge, for example, was not reached until the Ge content in the unfiltered spectrum was <0.16%. (T.G.)

Automated sub-5 nm image registration in integrated correlative fluorescence and electron microscopy using cathodoluminescence pointers

Science.gov (United States)

Haring, Martijn T.; Liv, Nalan; Zonnevylle, A. Christiaan; Narvaez, Angela C.; Voortman, Lenard M.; Kruit, Pieter; Hoogenboom, Jacob P.

2017-03-01

In the biological sciences, data from fluorescence and electron microscopy is correlated to allow fluorescence biomolecule identification within the cellular ultrastructure and/or ultrastructural analysis following live-cell imaging. High-accuracy (sub-100 nm) image overlay requires the addition of fiducial markers, which makes overlay accuracy dependent on the number of fiducials present in the region of interest. Here, we report an automated method for light-electron image overlay at high accuracy, i.e. below 5 nm. Our method relies on direct visualization of the electron beam position in the fluorescence detection channel using cathodoluminescence pointers. We show that image overlay using cathodoluminescence pointers corrects for image distortions, is independent of user interpretation, and does not require fiducials, allowing image correlation with molecular precision anywhere on a sample.

Mapping the dynamical organization of the cell nucleus through fluorescence correlation spectroscopy.

Science.gov (United States)

Stortz, Martin; Angiolini, Juan; Mocskos, Esteban; Wolosiuk, Alejandro; Pecci, Adali; Levi, Valeria

2018-05-01

The hierarchical organization of the cell nucleus into specialized open reservoirs and the nucleoplasm overcrowding impose restrictions to the mobility of biomolecules and their interactions with nuclear targets. These properties determine that many nuclear functions such as transcription, replication, splicing or DNA repair are regulated by complex, dynamical processes that do not follow simple rules. Advanced fluorescence microscopy tools and, in particular, fluorescence correlation spectroscopy (FCS) provide complementary and exquisite information on the dynamics of fluorescent labeled molecules moving through the nuclear space and are helping us to comprehend the complexity of the nuclear structure. Here, we describe how FCS methods can be applied to reveal the dynamical organization of the nucleus in live cells. Specifically, we provide instructions for the preparation of cellular samples with fluorescent tagged proteins and detail how FCS can be easily instrumented in commercial confocal microscopes. In addition, we describe general rules to set the parameters for one and two-color experiments and the required controls for these experiments. Finally, we review the statistical analysis of the FCS data and summarize the use of numerical simulations as a complementary approach that helps us to understand the complex matrix of molecular interactions network within the nucleus. Copyright © 2017 Elsevier Inc. All rights reserved.

PyCorrFit-generic data evaluation for fluorescence correlation spectroscopy.

Science.gov (United States)

Müller, Paul; Schwille, Petra; Weidemann, Thomas

2014-09-01

We present a graphical user interface (PyCorrFit) for the fitting of theoretical model functions to experimental data obtained by fluorescence correlation spectroscopy (FCS). The program supports many data file formats and features a set of tools specialized in FCS data evaluation. The Python source code is freely available for download from the PyCorrFit web page at http://pycorrfit.craban.de. We offer binaries for Ubuntu Linux, Mac OS X and Microsoft Windows. © The Author 2014. Published by Oxford University Press.

Quantifying the range of cross-correlated fluctuations using a q- L dependent AHXA coefficient

Science.gov (United States)

Wang, Fang; Wang, Lin; Chen, Yuming

2018-03-01

Recently, based on analogous height cross-correlation analysis (AHXA), a cross-correlation coefficient ρ×(L) has been proposed to quantify the levels of cross-correlation on different temporal scales for bivariate series. A limitation of this coefficient is that it cannot capture the full information of cross-correlations on amplitude of fluctuations. In fact, it only detects the cross-correlation at a specific order fluctuation, which might neglect some important information inherited from other order fluctuations. To overcome this disadvantage, in this work, based on the scaling of the qth order covariance and time delay L, we define a two-parameter dependent cross-correlation coefficient ρq(L) to detect and quantify the range and level of cross-correlations. This new version of ρq(L) coefficient leads to the formation of a ρq(L) surface, which not only is able to quantify the level of cross-correlations, but also allows us to identify the range of fluctuation amplitudes that are correlated in two given signals. Applications to the classical ARFIMA models and the binomial multifractal series illustrate the feasibility of this new coefficient ρq(L) . In addition, a statistical test is proposed to quantify the existence of cross-correlations between two given series. Applying our method to the real life empirical data from the 1999-2000 California electricity market, we find that the California power crisis in 2000 destroys the cross-correlation between the price and the load series but does not affect the correlation of the load series during and before the crisis.

Multifractal detrended Cross Correlation Analysis of Foreign Exchange and SENSEX fluctuation in Indian perspective

Science.gov (United States)

Dutta, Srimonti; Ghosh, Dipak; Chatterjee, Sucharita

2016-12-01

The manuscript studies autocorrelation and cross correlation of SENSEX fluctuations and Forex Exchange Rate in respect to Indian scenario. Multifractal detrended fluctuation analysis (MFDFA) and multifractal detrended cross correlation analysis (MFDXA) were employed to study the correlation between the two series. It was observed that the two series are strongly cross correlated. The change of degree of cross correlation with time was studied and the results are interpreted qualitatively.

Modeling CMB lensing cross correlations with CLEFT

Energy Technology Data Exchange (ETDEWEB)

Modi, Chirag; White, Martin [Department of Physics, University of California, Berkeley, CA 94720 (United States); Vlah, Zvonimir, E-mail: modichirag@berkeley.edu, E-mail: mwhite@berkeley.edu, E-mail: zvlah@stanford.edu [Stanford Institute for Theoretical Physics and Department of Physics, Stanford University, Stanford, CA 94306 (United States)

2017-08-01

A new generation of surveys will soon map large fractions of sky to ever greater depths and their science goals can be enhanced by exploiting cross correlations between them. In this paper we study cross correlations between the lensing of the CMB and biased tracers of large-scale structure at high z . We motivate the need for more sophisticated bias models for modeling increasingly biased tracers at these redshifts and propose the use of perturbation theories, specifically Convolution Lagrangian Effective Field Theory (CLEFT). Since such signals reside at large scales and redshifts, they can be well described by perturbative approaches. We compare our model with the current approach of using scale independent bias coupled with fitting functions for non-linear matter power spectra, showing that the latter will not be sufficient for upcoming surveys. We illustrate our ideas by estimating σ{sub 8} from the auto- and cross-spectra of mock surveys, finding that CLEFT returns accurate and unbiased results at high z . We discuss uncertainties due to the redshift distribution of the tracers, and several avenues for future development.

The effects of common risk factors on stock returns: A detrended cross-correlation analysis

Science.gov (United States)

Ruan, Qingsong; Yang, Bingchan

2017-10-01

In this paper, we investigate the cross-correlations between Fama and French three factors and the return of American industries on the basis of cross-correlation statistic test and multifractal detrended cross-correlation analysis (MF-DCCA). Qualitatively, we find that the return series of Fama and French three factors and American industries were overall significantly cross-correlated based on the analysis of a statistic. Quantitatively, we find that the cross-correlations between three factors and the return of American industries were strongly multifractal, and applying MF-DCCA we also investigate the cross-correlation of industry returns and residuals. We find that there exists multifractality of industry returns and residuals. The result of correlation coefficients we can verify that there exist other factors which influence the industry returns except Fama three factors.

Cross-Correlations between Energy and Emissions Markets: New Evidence from Fractal and Multifractal Analysis

Directory of Open Access Journals (Sweden)

Gang-Jin Wang

2014-01-01

Full Text Available We supply a new perspective to describe and understand the behavior of cross-correlations between energy and emissions markets. Namely, we investigate cross-correlations between oil and gas (Oil-Gas, oil and CO2 (Oil-CO2, and gas and CO2 (Gas-CO2 based on fractal and multifractal analysis. We focus our study on returns of the oil, gas, and CO2 during the period of April 22, 2005–April 30, 2013. In the empirical analysis, by using the detrended cross-correlation analysis (DCCA method, we find that cross-correlations for Oil-Gas, Oil-CO2, and Gas-CO2 obey a power-law and are weakly persistent. Then, we adopt the method of DCCA cross-correlation coefficient to quantify cross-correlations between energy and emissions markets. The results show that their cross-correlations are diverse at different time scales. Next, based on the multifractal DCCA method, we find that cross-correlated markets have the nonlinear and multifractal nature and that the multifractality strength for three cross-correlated markets is arranged in the order of Gas-CO2 > Oil-Gas > Oil-CO2. Finally, by employing the rolling windows method, which can be used to investigate time-varying cross-correlation scaling exponents, we analyze short-term and long-term market dynamics and find that the recent global financial crisis has a notable influence on short-term and long-term market dynamics.

Fluorescence correlation spectroscopy analysis for accurate determination of proportion of doubly labeled DNA in fluorescent DNA pool for quantitative biochemical assays.

Science.gov (United States)

Hou, Sen; Sun, Lili; Wieczorek, Stefan A; Kalwarczyk, Tomasz; Kaminski, Tomasz S; Holyst, Robert

2014-01-15

Fluorescent double-stranded DNA (dsDNA) molecules labeled at both ends are commonly produced by annealing of complementary single-stranded DNA (ssDNA) molecules, labeled with fluorescent dyes at the same (3' or 5') end. Because the labeling efficiency of ssDNA is smaller than 100%, the resulting dsDNA have two, one or are without a dye. Existing methods are insufficient to measure the percentage of the doubly-labeled dsDNA component in the fluorescent DNA sample and it is even difficult to distinguish the doubly-labeled DNA component from the singly-labeled component. Accurate measurement of the percentage of such doubly labeled dsDNA component is a critical prerequisite for quantitative biochemical measurements, which has puzzled scientists for decades. We established a fluorescence correlation spectroscopy (FCS) system to measure the percentage of doubly labeled dsDNA (PDL) in the total fluorescent dsDNA pool. The method is based on comparative analysis of the given sample and a reference dsDNA sample prepared by adding certain amount of unlabeled ssDNA into the original ssDNA solution. From FCS autocorrelation functions, we obtain the number of fluorescent dsDNA molecules in the focal volume of the confocal microscope and PDL. We also calculate the labeling efficiency of ssDNA. The method requires minimal amount of material. The samples have the concentration of DNA in the nano-molar/L range and the volume of tens of microliters. We verify our method by using restriction enzyme Hind III to cleave the fluorescent dsDNA. The kinetics of the reaction depends strongly on PDL, a critical parameter for quantitative biochemical measurements. Copyright © 2013 Elsevier B.V. All rights reserved.

Fluorescence detection of single molecules using pulsed near-field optical excitation and time correlated photon counting

International Nuclear Information System (INIS)

Ambrose, W.P.; Goodwin, P.M.; Martin, J.C.; Keller, R.A.

1994-01-01

Pulsed excitation, time correlated single photon counting and time gated detection are used in near-field optical microscopy to enhance fluorescence images and measure the fluorescence lifetimes of single molecules of Rhodamine 6G on silica surfaces. Time gated detection is used to reject prompt scattered background and to improve the image signal to noise ratio. The excited state lifetime of a single Rhodamine 6G molecule is found to depend on the position of the near-field probe. We attribute the lifetime variations to spontaneous emission rate alterations by the fluorescence reflected from and quenching by the aluminum coated probe

Stationary echo canceling in velocity estimation by time-domain cross-correlation

DEFF Research Database (Denmark)

Jensen, Jørgen Arendt

1993-01-01

The application of stationary echo canceling to ultrasonic estimation of blood velocities using time-domain cross-correlation is investigated. Expressions are derived that show the influence from the echo canceler on the signals that enter the cross-correlation estimator. It is demonstrated...

Fluorescence spectroscopic study of the aggregation behavior of non-cross-linked and cross-linked poly(alkylmethyldiallylammonium bromides) having decyl, octyl, and hexyl side chains in aqueous solution

NARCIS (Netherlands)

Wang, G.J; Engberts, J.B.F.N.

1996-01-01

The conformational state of a series of non-cross-linked and cross-linked poly(alkylmethyldiallylammonium bromides) bearing decyl, octyl, and hexyl side chains ((CL)-CopolC1-10, (CL)-CopolC1-8, and (CL)-CopolC1-6, respectively) in aqueous solutions were investigated by fluorescence spectroscopy

A correlative study of Papanicolaou smear, fluorescent antibody, and culture for the diagnosis of Chlamydia trachomatis.

Science.gov (United States)

Spence, M R; Barbacci, M; Kappus, E; Quinn, T

1986-11-01

A prospective study of 300 patients undergoing therapeutic termination of pregnancy was conducted. A Papanicolaou smear was obtained and a clinical evaluation of the cervix was made. Specimens from the cervix were examined by both direct fluorescent antibody and culture techniques for the presence of Chlamydia trachomatis. The presence of inflammation on Papanicolaou smear could be correlated with C trachomatis isolation. Papanicolaou smear findings consistent with C trachomatis lacked both sensitivity and specificity when compared with direct fluorescent antibody and/or culture techniques. A correlation was found between the clinical diagnosis of cervicitis and C trachomatis. This interrelationship was absent when the component findings of cervicitis (ectopy, friability, and purulent mucus) were examined independently.

Volatility-constrained multifractal detrended cross-correlation analysis: Cross-correlation among Mainland China, US, and Hong Kong stock markets

Science.gov (United States)

Cao, Guangxi; Zhang, Minjia; Li, Qingchen

2017-04-01

This study focuses on multifractal detrended cross-correlation analysis of the different volatility intervals of Mainland China, US, and Hong Kong stock markets. A volatility-constrained multifractal detrended cross-correlation analysis (VC-MF-DCCA) method is proposed to study the volatility conductivity of Mainland China, US, and Hong Kong stock markets. Empirical results indicate that fluctuation may be related to important activities in real markets. The Hang Seng Index (HSI) stock market is more influential than the Shanghai Composite Index (SCI) stock market. Furthermore, the SCI stock market is more influential than the Dow Jones Industrial Average stock market. The conductivity between the HSI and SCI stock markets is the strongest. HSI was the most influential market in the large fluctuation interval of 1991 to 2014. The autoregressive fractionally integrated moving average method is used to verify the validity of VC-MF-DCCA. Results show that VC-MF-DCCA is effective.

Exploiting Cross Correlations and Joint Analyses

Energy Technology Data Exchange (ETDEWEB)

Rhodes, J. [Caltech; Allen, S. [SLAC; Benson, B. A. [Chicago U., Astron. Astrophys. Ctr.; Chang, T. [Taipei, Inst. Astron. Astrophys.; de Putter, R. [Caltech; Dodelson, S. [Chicago U., Astron. Astrophys. Ctr.; Doré, O. [Caltech; Honscheid, K. [Ohio State U., CCAPP; Linder, E. [UC, Berkeley; Ménard, B. [Tokyo U., IPMU; Newman, J. [Pittsburgh U.; Nord, B. [Fermilab; Rozo, E. [SLAC; Rykoff, E. [SLAC; Vallinotto, A. [LBL, Berkeley; Weinberg, D. [Ohio State U., CCAPP

2014-02-28

In this report, we present a wide variety of ways in which information from multiple probes of dark energy may be combined to obtain additional information not accessible when they are considered separately. Fundamentally, because all major probes are affected by the underlying distribution of matter in the regions studied, there exist covariances between them that can provide information on cosmology. Combining multiple probes allows for more accurate (less contaminated by systematics) and more precise (since there is cosmological information encoded in cross-correlation statistics) measurements of dark energy. The potential of cross-correlation methods is only beginning to be realized. By bringing in information from other wavelengths, the capabilities of the existing probes of dark energy can be enhanced and systematic effects can be mitigated further. We present a mixture of work in progress and suggestions for future scientific efforts. Given the scope of future dark energy experiments, the greatest gains may only be realized with more coordination and cooperation between multiple project teams; we recommend that this interchange should begin sooner, rather than later, to maximize scientific gains.

Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy.

Science.gov (United States)

Laňková, Martina; Humpolíčková, Jana; Vosolsobě, Stanislav; Cit, Zdeněk; Lacek, Jozef; Čovan, Martin; Čovanová, Milada; Hof, Martin; Petrášek, Jan

2016-04-01

A number of fluorescence microscopy techniques are described to study dynamics of fluorescently labeled proteins, lipids, nucleic acids, and whole organelles. However, for studies of plant plasma membrane (PM) proteins, the number of these techniques is still limited because of the high complexity of processes that determine the dynamics of PM proteins and the existence of cell wall. Here, we report on the usage of raster image correlation spectroscopy (RICS) for studies of integral PM proteins in suspension-cultured tobacco cells and show its potential in comparison with the more widely used fluorescence recovery after photobleaching method. For RICS, a set of microscopy images is obtained by single-photon confocal laser scanning microscopy (CLSM). Fluorescence fluctuations are subsequently correlated between individual pixels and the information on protein mobility are extracted using a model that considers processes generating the fluctuations such as diffusion and chemical binding reactions. As we show here using an example of two integral PM transporters of the plant hormone auxin, RICS uncovered their distinct short-distance lateral mobility within the PM that is dependent on cytoskeleton and sterol composition of the PM. RICS, which is routinely accessible on modern CLSM instruments, thus represents a valuable approach for studies of dynamics of PM proteins in plants.

Correlation of electronic carotenoid-chlorophyll interactions and fluorescence quenching with the aggregation of native LHC II and chlorophyll deficient mutants

International Nuclear Information System (INIS)

Liao, Pen-Nan; Bode, Stefan; Wilk, Laura; Hafi, Nour; Walla, Peter J.

2010-01-01

The aggregation dependent correlation between fluorescence quenching and the electronic carotenoid-chlorophyll interactions, φ Coupling Car S 1 -Chl , as measured by comparing chlorophyll fluorescence observed after two- and one-photon excitation, has been investigated using native LHC II samples as well as mutants lacking Chl 2 and Chl 13. For native LHC II the same linear correlation between φ Coupling Car S 1 -Chl and the fluorescence quenching was observed as previously reported for the pH and Zea-dependent quenching of LHC II . In order to elucidate which carotenoid-chlorophyll pair might dominate this correlation we also investigated the mutants lacking Chl 2 and Chl 13. However, also with these mutants the same linear correlation as for native LHC II was observed. This provides indication that these two chlorophylls play only a minor role for the observed effects. Nevertheless, we also conclude that this does not exclude that their neighboured carotenoids, lutein 1 and neoxanthin, might interact electronically with other chlorophylls close by.

Dynamic evolution of cross-correlations in the Chinese stock market.

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Fei Ren

Full Text Available The analysis of cross-correlations is extensively applied for the understanding of interconnections in stock markets and the portfolio risk estimation. Current studies of correlations in Chinese market mainly focus on the static correlations between return series, and this calls for an urgent need to investigate their dynamic correlations. Our study aims to reveal the dynamic evolution of cross-correlations in the Chinese stock market, and offer an exact interpretation for the evolution behavior. The correlation matrices constructed from the return series of 367 A-share stocks traded on the Shanghai Stock Exchange from January 4, 1999 to December 30, 2011 are calculated over a moving window with a size of 400 days. The evolutions of the statistical properties of the correlation coefficients, eigenvalues, and eigenvectors of the correlation matrices are carefully analyzed. We find that the stock correlations are significantly increased in the periods of two market crashes in 2001 and 2008, during which only five eigenvalues significantly deviate from the random correlation matrix, and the systemic risk is higher in these volatile periods than calm periods. By investigating the significant contributors of the deviating eigenvectors in different time periods, we observe a dynamic evolution behavior in business sectors such as IT, electronics, and real estate, which lead the rise (drop before (after the crashes. Our results provide new perspectives for the understanding of the dynamic evolution of cross-correlations in the Chines stock markets, and the result of risk estimation is valuable for the application of risk management.

Dynamic evolution of cross-correlations in the Chinese stock market.

Science.gov (United States)

Ren, Fei; Zhou, Wei-Xing

2014-01-01

The analysis of cross-correlations is extensively applied for the understanding of interconnections in stock markets and the portfolio risk estimation. Current studies of correlations in Chinese market mainly focus on the static correlations between return series, and this calls for an urgent need to investigate their dynamic correlations. Our study aims to reveal the dynamic evolution of cross-correlations in the Chinese stock market, and offer an exact interpretation for the evolution behavior. The correlation matrices constructed from the return series of 367 A-share stocks traded on the Shanghai Stock Exchange from January 4, 1999 to December 30, 2011 are calculated over a moving window with a size of 400 days. The evolutions of the statistical properties of the correlation coefficients, eigenvalues, and eigenvectors of the correlation matrices are carefully analyzed. We find that the stock correlations are significantly increased in the periods of two market crashes in 2001 and 2008, during which only five eigenvalues significantly deviate from the random correlation matrix, and the systemic risk is higher in these volatile periods than calm periods. By investigating the significant contributors of the deviating eigenvectors in different time periods, we observe a dynamic evolution behavior in business sectors such as IT, electronics, and real estate, which lead the rise (drop) before (after) the crashes. Our results provide new perspectives for the understanding of the dynamic evolution of cross-correlations in the Chines stock markets, and the result of risk estimation is valuable for the application of risk management.

Cross-Correlation-Function-Based Multipath Mitigation Method for Sine-BOC Signals

Directory of Open Access Journals (Sweden)

H. H. Chen

2012-06-01

Full Text Available Global Navigation Satellite Systems (GNSS positioning accuracy indoor and urban canyons environments are greatly affected by multipath due to distortions in its autocorrelation function. In this paper, a cross-correlation function between the received sine phased Binary Offset Carrier (sine-BOC modulation signal and the local signal is studied firstly, and a new multipath mitigation method based on cross-correlation function for sine-BOC signal is proposed. This method is implemented to create a cross-correlation function by designing the modulated symbols of the local signal. The theoretical analysis and simulation results indicate that the proposed method exhibits better multipath mitigation performance compared with the traditional Double Delta Correlator (DDC techniques, especially the medium/long delay multipath signals, and it is also convenient and flexible to implement by using only one correlator, which is the case of low-cost mass-market receivers.

Cross Correlation versus Normalized Mutual Information on Image Registration

Science.gov (United States)

Tan, Bin; Tilton, James C.; Lin, Guoqing

2016-01-01

This is the first study to quantitatively assess and compare cross correlation and normalized mutual information methods used to register images in subpixel scale. The study shows that the normalized mutual information method is less sensitive to unaligned edges due to the spectral response differences than is cross correlation. This characteristic makes the normalized image resolution a better candidate for band to band registration. Improved band-to-band registration in the data from satellite-borne instruments will result in improved retrievals of key science measurements such as cloud properties, vegetation, snow and fire.

Detrended fluctuation analysis made flexible to detect range of cross-correlated fluctuations

Science.gov (United States)

Kwapień, Jarosław; Oświecimka, Paweł; DroŻdŻ, Stanisław

2015-11-01

The detrended cross-correlation coefficient ρDCCA has recently been proposed to quantify the strength of cross-correlations on different temporal scales in bivariate, nonstationary time series. It is based on the detrended cross-correlation and detrended fluctuation analyses (DCCA and DFA, respectively) and can be viewed as an analog of the Pearson coefficient in the case of the fluctuation analysis. The coefficient ρDCCA works well in many practical situations but by construction its applicability is limited to detection of whether two signals are generally cross-correlated, without the possibility to obtain information on the amplitude of fluctuations that are responsible for those cross-correlations. In order to introduce some related flexibility, here we propose an extension of ρDCCA that exploits the multifractal versions of DFA and DCCA: multifractal detrended fluctuation analysis and multifractal detrended cross-correlation analysis, respectively. The resulting new coefficient ρq not only is able to quantify the strength of correlations but also allows one to identify the range of detrended fluctuation amplitudes that are correlated in two signals under study. We show how the coefficient ρq works in practical situations by applying it to stochastic time series representing processes with long memory: autoregressive and multiplicative ones. Such processes are often used to model signals recorded from complex systems and complex physical phenomena like turbulence, so we are convinced that this new measure can successfully be applied in time-series analysis. In particular, we present an example of such application to highly complex empirical data from financial markets. The present formulation can straightforwardly be extended to multivariate data in terms of the q -dependent counterpart of the correlation matrices and then to the network representation.

Multifractal detrended cross-correlation analysis on gold, crude oil and foreign exchange rate time series

Science.gov (United States)

Pal, Mayukha; Madhusudana Rao, P.; Manimaran, P.

2014-12-01

We apply the recently developed multifractal detrended cross-correlation analysis method to investigate the cross-correlation behavior and fractal nature between two non-stationary time series. We analyze the daily return price of gold, West Texas Intermediate and Brent crude oil, foreign exchange rate data, over a period of 18 years. The cross correlation has been measured from the Hurst scaling exponents and the singularity spectrum quantitatively. From the results, the existence of multifractal cross-correlation between all of these time series is found. We also found that the cross correlation between gold and oil prices possess uncorrelated behavior and the remaining bivariate time series possess persistent behavior. It was observed for five bivariate series that the cross-correlation exponents are less than the calculated average generalized Hurst exponents (GHE) for q0 and for one bivariate series the cross-correlation exponent is greater than GHE for all q values.

ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy [version 1; referees: 2 approved, 1 approved with reservations

Directory of Open Access Journals (Sweden)

Elisabeth Brama

2016-12-01

Full Text Available In-resin fluorescence (IRF protocols preserve fluorescent proteins in resin-embedded cells and tissues for correlative light and electron microscopy, aiding interpretation of macromolecular function within the complex cellular landscape. Dual-contrast IRF samples can be imaged in separate fluorescence and electron microscopes, or in dual-modality integrated microscopes for high resolution correlation of fluorophore to organelle. IRF samples also offer a unique opportunity to automate correlative imaging workflows. Here we present two new locator tools for finding and following fluorescent cells in IRF blocks, enabling future automation of correlative imaging. The ultraLM is a fluorescence microscope that integrates with an ultramicrotome, which enables ‘smart collection’ of ultrathin sections containing fluorescent cells or tissues for subsequent transmission electron microscopy or array tomography. The miniLM is a fluorescence microscope that integrates with serial block face scanning electron microscopes, which enables ‘smart tracking’ of fluorescent structures during automated serial electron image acquisition from large cell and tissue volumes.

Inducing fluorescence of uranyl acetate as a dual-purpose contrast agent for correlative light-electron microscopy with nanometre precision.

Science.gov (United States)

Tuijtel, Maarten W; Mulder, Aat A; Posthuma, Clara C; van der Hoeven, Barbara; Koster, Abraham J; Bárcena, Montserrat; Faas, Frank G A; Sharp, Thomas H

2017-09-05

Correlative light-electron microscopy (CLEM) combines the high spatial resolution of transmission electron microscopy (TEM) with the capability of fluorescence light microscopy (FLM) to locate rare or transient cellular events within a large field of view. CLEM is therefore a powerful technique to study cellular processes. Aligning images derived from both imaging modalities is a prerequisite to correlate the two microscopy data sets, and poor alignment can limit interpretability of the data. Here, we describe how uranyl acetate, a commonly-used contrast agent for TEM, can be induced to fluoresce brightly at cryogenic temperatures (-195 °C) and imaged by cryoFLM using standard filter sets. This dual-purpose contrast agent can be used as a general tool for CLEM, whereby the equivalent staining allows direct correlation between fluorescence and TEM images. We demonstrate the potential of this approach by performing multi-colour CLEM of cells containing equine arteritis virus proteins tagged with either green- or red-fluorescent protein, and achieve high-precision localization of virus-induced intracellular membrane modifications. Using uranyl acetate as a dual-purpose contrast agent, we achieve an image alignment precision of ~30 nm, twice as accurate as when using fiducial beads, which will be essential for combining TEM with the evolving field of super-resolution light microscopy.

Multiscale properties of DNA primary structure: cross-scale correlations

International Nuclear Information System (INIS)

Altajskij, M.V.; Ivanov, V.V.; Polozov, R.V.

2000-01-01

Cross-scale correlations of wavelet coefficients of the DNA coding sequences are calculated and compared to that of the generated random sequence of the same length. The coding sequences are shown to have strong correlation between large and small scale structures, while random sequences have not

Probing the photoluminescence properties of gold nanoclusters by fluorescence lifetime correlation spectroscopy

International Nuclear Information System (INIS)

Yuan, C. T.; Lin, T. N.; Shen, J. L.; Lin, C. A.; Chang, W. H.; Cheng, H. W.; Tang, J.

2013-01-01

Gold nanoclusters (Au NCs) have attracted much attention for promising applications in biological imaging owing to their tiny sizes and biocompatibility. So far, most efforts have been focused on the strategies for fabricating high-quality Au NCs and then characterized by conventional ensemble measurement. Here, a fusion single-molecule technique combining fluorescence correlation spectroscopy and time-correlated single-photon counting can be successfully applied to probe the photoluminescence (PL) properties for sparse Au NCs. In this case, the triplet-state dynamics and diffusion process can be observed simultaneously and the relevant time constants can be derived. This work provides a complementary insight into the PL mechanism at the molecular levels for Au NCs in solution

Spatio-energetic cross talk in photon counting detectors: Detector model and correlated Poisson data generator.

Science.gov (United States)

Taguchi, Katsuyuki; Polster, Christoph; Lee, Okkyun; Stierstorfer, Karl; Kappler, Steffen

2016-12-01

An x-ray photon interacts with photon counting detectors (PCDs) and generates an electron charge cloud or multiple clouds. The clouds (thus, the photon energy) may be split between two adjacent PCD pixels when the interaction occurs near pixel boundaries, producing a count at both of the pixels. This is called double-counting with charge sharing. (A photoelectric effect with K-shell fluorescence x-ray emission would result in double-counting as well). As a result, PCD data are spatially and energetically correlated, although the output of individual PCD pixels is Poisson distributed. Major problems include the lack of a detector noise model for the spatio-energetic cross talk and lack of a computationally efficient simulation tool for generating correlated Poisson data. A Monte Carlo (MC) simulation can accurately simulate these phenomena and produce noisy data; however, it is not computationally efficient. In this study, the authors developed a new detector model and implemented it in an efficient software simulator that uses a Poisson random number generator to produce correlated noisy integer counts. The detector model takes the following effects into account: (1) detection efficiency; (2) incomplete charge collection and ballistic effect; (3) interaction with PCDs via photoelectric effect (with or without K-shell fluorescence x-ray emission, which may escape from the PCDs or be reabsorbed); and (4) electronic noise. The correlation was modeled by using these two simplifying assumptions: energy conservation and mutual exclusiveness. The mutual exclusiveness is that no more than two pixels measure energy from one photon. The effect of model parameters has been studied and results were compared with MC simulations. The agreement, with respect to the spectrum, was evaluated using the reduced χ 2 statistics or a weighted sum of squared errors, χ red 2 (≥1), where χ red 2 =1 indicates a perfect fit. The model produced spectra with flat field irradiation that

Hidden cross-correlation patterns in stock markets based on permutation cross-sample entropy and PCA

Science.gov (United States)

Lin, Aijing; Shang, Pengjian; Zhong, Bo

2014-12-01

In this article, we investigate the hidden cross-correlation structures in Chinese stock markets and US stock markets by performing PCSE combined with PCA approach. It is suggested that PCSE can provide a more faithful and more interpretable description of the dynamic mechanism between time series than cross-correlation matrix. We show that this new technique can be adapted to observe stock markets especially during financial crisis. In order to identify and compare the interactions and structures of stock markets during financial crisis, as well as in normal periods, all the samples are divided into four sub-periods. The results imply that the cross-correlations between Chinese group are stronger than the US group in the most sub-periods. In particular, it is likely that the US stock markets are more integrated with each other during global financial crisis than during Asian financial crisis. However, our results illustrate that Chinese stock markets are not immune from the global financial crisis, although less integrated with other markets if they are compared with US stock markets.

Cross-Correlation Asymmetries and Causal Relationships between Stock and Market Risk

Science.gov (United States)

Borysov, Stanislav S.; Balatsky, Alexander V.

2014-01-01

We study historical correlations and lead-lag relationships between individual stock risk (volatility of daily stock returns) and market risk (volatility of daily returns of a market-representative portfolio) in the US stock market. We consider the cross-correlation functions averaged over all stocks, using 71 stock prices from the Standard & Poor's 500 index for 1994–2013. We focus on the behavior of the cross-correlations at the times of financial crises with significant jumps of market volatility. The observed historical dynamics showed that the dependence between the risks was almost linear during the US stock market downturn of 2002 and after the US housing bubble in 2007, remaining at that level until 2013. Moreover, the averaged cross-correlation function often had an asymmetric shape with respect to zero lag in the periods of high correlation. We develop the analysis by the application of the linear response formalism to study underlying causal relations. The calculated response functions suggest the presence of characteristic regimes near financial crashes, when the volatility of an individual stock follows the market volatility and vice versa. PMID:25162697

Cross-correlation asymmetries and causal relationships between stock and market risk.

Science.gov (United States)

Borysov, Stanislav S; Balatsky, Alexander V

2014-01-01

We study historical correlations and lead-lag relationships between individual stock risk (volatility of daily stock returns) and market risk (volatility of daily returns of a market-representative portfolio) in the US stock market. We consider the cross-correlation functions averaged over all stocks, using 71 stock prices from the Standard & Poor's 500 index for 1994-2013. We focus on the behavior of the cross-correlations at the times of financial crises with significant jumps of market volatility. The observed historical dynamics showed that the dependence between the risks was almost linear during the US stock market downturn of 2002 and after the US housing bubble in 2007, remaining at that level until 2013. Moreover, the averaged cross-correlation function often had an asymmetric shape with respect to zero lag in the periods of high correlation. We develop the analysis by the application of the linear response formalism to study underlying causal relations. The calculated response functions suggest the presence of characteristic regimes near financial crashes, when the volatility of an individual stock follows the market volatility and vice versa.

Angle-correlated cross sections in the framework of the continuum shell model

International Nuclear Information System (INIS)

Moerschel, K.P.

1984-01-01

In the present thesis in the framework of the continuum shell modell a concept for the treatment of angle-correlated cross sections was developed by which coincidence experiments on electron scattering on nuclei are described. For this the existing Darmstadt continuum-shell-model code had to be extended to the calculation of the correlation coefficients in which nuclear dynamics enter and which determine completely the angle-correlated cross sections. Under inclusion of the kinematics a method for the integration over the scattered electron was presented and used for the comparison with corresponding experiments. As application correlation coefficients for the proton channel in 12 C with 1 - and 2 + excitations were studied. By means of these coefficients finally cross sections for the reaction 12 C (e,p) 11 B could be calculated and compared with the experiment whereby the developed methods were proved as suitable to predict correctly both the slope and the quantity of the experimental cross sections. (orig.) [de

The Dynamic Cross-Correlations between Mass Media News, New Media News, and Stock Returns

Directory of Open Access Journals (Sweden)

Zuochao Zhang

2018-01-01

Full Text Available We investigate the dynamic cross-correlations between mass media news, new media news, and stock returns for the SSE 50 Index in Chinese stock market by employing the MF-DCCA method. The empirical results show that (1 there exist power-law cross-correlations between two types of news as well as between news and its corresponding SSE 50 Index return; (2 the cross-correlations between mass media news and SSE 50 Index returns show larger multifractality and more complicated structures; (3 mass media news and new media news have both complementary and competitive relationships; (4 with the rolling window analysis, we further find that there is a general increasing trend for the cross-correlations between the two types of news as well as the cross-correlations between news and returns and this trend becomes more persistent over time.

Characterization of protein adsorption onto FePt nanoparticles using dual-focus fluorescence correlation spectroscopy

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Pauline Maffre

2011-07-01

Full Text Available Using dual-focus fluorescence correlation spectroscopy, we have analyzed the adsorption of three human blood serum proteins, namely serum albumin, apolipoprotein A-I and apolipoprotein E4, onto polymer-coated, fluorescently labeled FePt nanoparticles (~12 nm diameter carrying negatively charged carboxyl groups on their surface. For all three proteins, a step-wise increase in hydrodynamic radius with protein concentration was observed, strongly suggesting the formation of protein monolayers that enclose the nanoparticles. Consistent with this interpretation, the absolute increase in hydrodynamic radius can be correlated with the molecular shapes of the proteins known from X-ray crystallography and solution experiments, indicating that the proteins bind on the nanoparticles in specific orientations. The equilibrium dissociation coefficients, measuring the affinity of the proteins to the nanoparticles, were observed to differ by almost four orders of magnitude. These variations can be understood in terms of the electrostatic properties of the proteins. From structure-based calculations of the surface potentials, positively charged patches of different extents can be revealed, through which the proteins interact electrostatically with the negatively charged nanoparticle surfaces.

In situ fluorescence spectroscopy correlates ionomer degradation to reactive oxygen species generation in an operating fuel cell.

Science.gov (United States)

Prabhakaran, Venkateshkumar; Arges, Christopher G; Ramani, Vijay

2013-11-21

The rate of generation of reactive oxygen species (ROS) within the polymer electrolyte membrane (PEM) of an operating proton exchange member fuel cell (PEMFC) was monitored using in situ fluorescence spectroscopy. A modified barrier layer was introduced between the PEM and the electrocatalyst layer to eliminate metal-dye interactions and fluorescence resonance energy transfer (FRET) effects during measurements. Standard fuel cell operating parameters (temperature, relative humidity, and electrode potential) were systematically varied to evaluate their influence on the rate of ROS generation during PEMFC operation. Independently, the macroscopic rate of PEM degradation was measured by monitoring the fluoride ion emission rate (FER) in the effluent stream at each operating condition. The ROS generation reaction rate constant (estimated from the in situ fluorescence experiments) correlated perfectly with the measured FER across all conditions, demonstrating unequivocally for the first time that a direct correlation exists between in situ ROS generation and PEM macroscopic degradation. The activation energy for ROS generation within the PEM was estimated to be 12.5 kJ mol(-1).

Sodium boiling detection in LMFBRs by acoustic-neutronic cross correlation

International Nuclear Information System (INIS)

Wright, S.A.

1977-01-01

The acoustic and neutronic noise signals caused by boiling are the signals primarily considered likely to detect sodium boiling in an LMFBR. Unfortunately, these signals may have serious signal-to-noise problems due to strong background noise sources. Neutronic-acoustic cross correlation techniques are expected to provide a means of improving the signal-to-noise ratio. This technique can improve the signal-to-noise ratio because the neutronic and acoustic signals due to boiling are highly correlated near the bubble repetition frequency, while the background noise sources are expected to be uncorrelated (or at most weakly correlated). An experiment was designed to show that the neutronic and acoustic noise signals are indeed highly correlated. The experiment consisted of simulating the void and pressure effects of local sodium boiling in the core of a zero-power reactor (ARK). The analysis showed that the neutronic and acoustic noise signals caused by boiling are almost perfectly correlated in a wide frequency band about the bubble repetition frequency. The results of the experiments were generalized to full-scale reactors to compare the inherent effectiveness of the methods which use the neutronic or acoustic signals alone with a hybrid method, which cross correlates the neutronic and acoustic signals. It was concluded that over a zone of the reactor where the void coefficient is sufficiently large (approximately 85 percent the core volume), the cross correlation method can provide a more rapid detection system for a given signal-to-noise ratio. However, where the void coefficient is small, one must probably rely on the acoustic method alone

Integrated Transmission Electron and Single‐Molecule Fluorescence Microscopy Correlates Reactivity with Ultrastructure in a Single Catalyst Particle

OpenAIRE

Hendriks, Frank C.; Mohammadian, Sajjad; Ristanović, Zoran; Kalirai, Sam; Meirer, Florian; Vogt, Eelco T. C.; Bruijnincx, Pieter C. A.; Gerritsen, Hans C.; Weckhuysen, Bert M.

2017-01-01

Abstract Establishing structure–activity relationships in complex, hierarchically structured nanomaterials, such as fluid catalytic cracking (FCC) catalysts, requires characterization with complementary, correlated analysis techniques. An integrated setup has been developed to perform transmission electron microscopy (TEM) and single‐molecule fluorescence (SMF) microscopy on such nanostructured samples. Correlated structure–reactivity information was obtained for 100 nm thin, microtomed secti...

On the performance of bioanalytical fluorescence correlation spectroscopy measurements in a multiparameter photon-counting microscope

Energy Technology Data Exchange (ETDEWEB)

Mazouchi, Amir; Liu Baoxu; Bahram, Abdullah [Department of Physics, Institute for Optical Sciences, University of Toronto, Toronto (Canada); Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Rd. N., Mississauga, ON, L5L 1C6 (Canada); Gradinaru, Claudiu C., E-mail: claudiu.gradinaru@utoronto.ca [Department of Physics, Institute for Optical Sciences, University of Toronto, Toronto (Canada); Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Rd. N., Mississauga, ON, L5L 1C6 (Canada)

2011-02-28

Fluorescence correlation spectroscopy (FCS) data acquisition and analysis routines were developed and implemented in a home-built, multiparameter photon-counting microscope. Laser excitation conditions were investigated for two representative fluorescent probes, Rhodamine110 and enhanced green fluorescent protein (EGFP). Reliable local concentrations and diffusion constants were obtained by fitting measured FCS curves, provided that the excitation intensity did not exceed 20% of the saturation level for each fluorophore. Accurate results were obtained from FCS measurements for sample concentrations varying from pM to {mu}M range, as well as for conditions of high background signals. These experimental constraints were found to be determined by characteristics of the detection system and by the saturation behavior of the fluorescent probes. These factors actually limit the average number of photons that can be collected from a single fluorophore passing through the detection volume. The versatility of our setup and the data analysis capabilities were tested by measuring the mobility of EGFP in the nucleus of Drosophila cells under conditions of high concentration and molecular crowding. As a bioanalytical application, we studied by FCS the binding affinity of a novel peptide-based drug to the cancer-regulating STAT3 protein and corroborated the results with fluorescence polarization analysis derived from the same photon data.

Needlet estimation of cross-correlation between CMB lensing maps and LSS

Energy Technology Data Exchange (ETDEWEB)

Bianchini, Federico [Astrophysics Sector, SISSA, Via Bonomea 265, I-34136 Trieste (Italy); Renzi, Alessandro; Marinucci, Domenico, E-mail: fbianchini@sissa.it, E-mail: renzi@mat.uniroma2.it, E-mail: marinucc@mat.uniroma2.it [Dipartimento di Matematica, Università di Roma Tor Vergata, Via della Ricerca Scientifica 1, 00133 Roma (Italy)

2016-11-01

In this paper we develop a novel needlet-based estimator to investigate the cross-correlation between cosmic microwave background (CMB) lensing maps and large-scale structure (LSS) data. We compare this estimator with its harmonic counterpart and, in particular, we analyze the bias effects of different forms of masking. In order to address this bias, we also implement a MASTER-like technique in the needlet case. The resulting estimator turns out to have an extremely good signal-to-noise performance. Our analysis aims at expanding and optimizing the operating domains in CMB-LSS cross-correlation studies, similarly to CMB needlet data analysis. It is motivated especially by next generation experiments (such as Euclid) which will allow us to derive much tighter constraints on cosmological and astrophysical parameters through cross-correlation measurements between CMB and LSS.

Balanced cross-rate model for saturated molecular fluorescence in flames using a nanosecond pulse length laser

International Nuclear Information System (INIS)

Lucht, R.P.; Sweeney, D.W.; Laurendeau, N.M.

1980-01-01

The balanced cross-rate model is proposed to analyze laser-induced molecular fluorescence signals when the laser pulse length is of the order of nanoseconds. Nanosecond pulse length lasers. specifically Q-switched Nd:YAG-pumped dye lasers, are attractive for saturated molecular fluorescence spectroscopy because of their high peak power and because of their short pulse length minimizes the risk of laser-induced chemistry. In the balanced cross-rate model, single upper and lower rotational levels are assumed to be directly coupled by the laser radiation. Because the laser-induced processes which couple these levels are so fast at saturation intensities, a steady state is established between the two levels within picoseconds. Provided that the total population of the two laser-coupled rotational levels is constant during the laser pulse, the total molecular population can be calculated from the observed upper rotational level population using a two-level saturation model and Boltzmann statistics. Numerical simulation of the laser excitation dynamics of OH in an atmospheric pressure H 2 /O 2 /N 2 flame indicates that the balanced cross-rate model will give accurate results provided that the rotational relaxation rates in the upper and lower sets of rotational levels are approximately equal

The Atacama Cosmology Telescope: cross correlation with Planck maps

Energy Technology Data Exchange (ETDEWEB)

Louis, Thibaut; Calabrese, Erminia; Dunkley, Joanna; Næss, Sigurd [Department of Astrophysics, Oxford University, Oxford OX1 3RH (United Kingdom); Addison, Graeme E.; Hincks, Adam D. [Department of Physics and Astronomy, University of British Columbia, Vancouver, BC V6T 1Z4 (Canada); Hasselfield, Matthew; Hlozek, Renée [Department of Astrophysical Sciences, Peyton Hall, Princeton University, Princeton, NJ 08544 (United States); Bond, J. Richard; Hajian, Amir [Canadian Institute for Theoretical Astrophysics, University of Toronto, Toronto, ON M5S 3H8 (Canada); Das, Sudeep [Argonne National Laboratory, 9700 S. Cass Ave., Lemont, IL 60439 (United States); Devlin, Mark J. [Department of Physics and Astronomy, University of Pennsylvania, 209 South 33rd Street, Philadelphia, PA 19104, U.S.A (United States); Dünner, Rolando; Infante, Leopoldo [Departamento de Astronomía y Astrofísica, Facultad de Física, Pontificia Universidad Católica de Chile, Casilla 306, Santiago 22 (Chile); Gralla, Megan; Marriage, Tobias A. [Dept. of Physics and Astronomy, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218-2686 (United States); Huffenberger, Kevin [Department of Physics, Florida State University, Keen Physics Building, 77 Chieftan Way, Tallahassee, Florida (United States); Kosowsky, Arthur [Department of Physics and Astronomy, University of Pittsburgh, Pittsburgh, PA, 15260 (United States); Moodley, Kavilan [Astrophysics and Cosmology Research Unit, School of Mathematical Sciences, University of KwaZulu-Natal, Durban, 4041 (South Africa); Niemack, Michael D., E-mail: Thibaut.Louis@astro.ox.ac.uk [Joseph Henry Laboratories of Physics, Jadwin Hall, Princeton University, Princeton, NJ 08544 (United States); and others

2014-07-01

We present the temperature power spectrum of the Cosmic Microwave Background obtained by cross-correlating maps from the Atacama Cosmology Telescope (ACT) at 148 and 218 GHz with maps from the Planck satellite at 143 and 217 GHz, in two overlapping regions covering 592 square degrees. We find excellent agreement between the two datasets at both frequencies, quantified using the variance of the residuals between the ACT power spectra and the ACT × Planck cross-spectra. We use these cross-correlations to measure the calibration of the ACT data at 148 and 218 GHz relative to Planck, to 0.7% and 2% precision respectively. We find no evidence for anisotropy in the calibration parameter. We compare the Planck 353 GHz power spectrum with the measured amplitudes of dust and cosmic infrared background (CIB) of ACT data at 148 and 218 GHz. We also compare planet and point source measurements from the two experiments.

Random matrix approach to cross correlations in financial data

Science.gov (United States)

Plerou, Vasiliki; Gopikrishnan, Parameswaran; Rosenow, Bernd; Amaral, Luís A.; Guhr, Thomas; Stanley, H. Eugene

2002-06-01

We analyze cross correlations between price fluctuations of different stocks using methods of random matrix theory (RMT). Using two large databases, we calculate cross-correlation matrices C of returns constructed from (i) 30-min returns of 1000 US stocks for the 2-yr period 1994-1995, (ii) 30-min returns of 881 US stocks for the 2-yr period 1996-1997, and (iii) 1-day returns of 422 US stocks for the 35-yr period 1962-1996. We test the statistics of the eigenvalues λi of C against a ``null hypothesis'' - a random correlation matrix constructed from mutually uncorrelated time series. We find that a majority of the eigenvalues of C fall within the RMT bounds [λ-,λ+] for the eigenvalues of random correlation matrices. We test the eigenvalues of C within the RMT bound for universal properties of random matrices and find good agreement with the results for the Gaussian orthogonal ensemble of random matrices-implying a large degree of randomness in the measured cross-correlation coefficients. Further, we find that the distribution of eigenvector components for the eigenvectors corresponding to the eigenvalues outside the RMT bound display systematic deviations from the RMT prediction. In addition, we find that these ``deviating eigenvectors'' are stable in time. We analyze the components of the deviating eigenvectors and find that the largest eigenvalue corresponds to an influence common to all stocks. Our analysis of the remaining deviating eigenvectors shows distinct groups, whose identities correspond to conventionally identified business sectors. Finally, we discuss applications to the construction of portfolios of stocks that have a stable ratio of risk to return.

The cross-correlation analysis of multi property of stock markets based on MM-DFA

Science.gov (United States)

Yang, Yujun; Li, Jianping; Yang, Yimei

2017-09-01

In this paper, we propose a new method called DH-MXA based on distribution histograms of Hurst surface and multiscale multifractal detrended fluctuation analysis. The method allows us to investigate the cross-correlation characteristics among multiple properties of different stock time series. It may provide a new way of measuring the nonlinearity of several signals. It also can provide a more stable and faithful description of cross-correlation of multiple properties of stocks. The DH-MXA helps us to present much richer information than multifractal detrented cross-correlation analysis and allows us to assess many universal and subtle cross-correlation characteristics of stock markets. We show DH-MXA by selecting four artificial data sets and five properties of four stock time series from different countries. The results show that our proposed method can be adapted to investigate the cross-correlation of stock markets. In general, the American stock markets are more mature and less volatile than the Chinese stock markets.

Detrended partial cross-correlation analysis of two nonstationary time series influenced by common external forces

Science.gov (United States)

Qian, Xi-Yuan; Liu, Ya-Min; Jiang, Zhi-Qiang; Podobnik, Boris; Zhou, Wei-Xing; Stanley, H. Eugene

2015-06-01

When common factors strongly influence two power-law cross-correlated time series recorded in complex natural or social systems, using detrended cross-correlation analysis (DCCA) without considering these common factors will bias the results. We use detrended partial cross-correlation analysis (DPXA) to uncover the intrinsic power-law cross correlations between two simultaneously recorded time series in the presence of nonstationarity after removing the effects of other time series acting as common forces. The DPXA method is a generalization of the detrended cross-correlation analysis that takes into account partial correlation analysis. We demonstrate the method by using bivariate fractional Brownian motions contaminated with a fractional Brownian motion. We find that the DPXA is able to recover the analytical cross Hurst indices, and thus the multiscale DPXA coefficients are a viable alternative to the conventional cross-correlation coefficient. We demonstrate the advantage of the DPXA coefficients over the DCCA coefficients by analyzing contaminated bivariate fractional Brownian motions. We calculate the DPXA coefficients and use them to extract the intrinsic cross correlation between crude oil and gold futures by taking into consideration the impact of the U.S. dollar index. We develop the multifractal DPXA (MF-DPXA) method in order to generalize the DPXA method and investigate multifractal time series. We analyze multifractal binomial measures masked with strong white noises and find that the MF-DPXA method quantifies the hidden multifractal nature while the multifractal DCCA method fails.

Integrated Transmission Electron and Single-Molecule Fluorescence Microscopy Correlates Reactivity with Ultrastructure in a Single Catalyst Particle

OpenAIRE

Hendriks, Frank C.; Mohammadian, Sajjad; Ristanovic, Zoran; Kalirai, Samanbir; Meirer, Florian; Vogt, Eelco T. C.; Bruijnincx, Pieter C. A.; Gerritsen, Hans; Weckhuysen, Bert M.

2018-01-01

Establishing structure–activity relationships in complex, hierarchically structured nanomaterials, such as fluid catalytic cracking (FCC) catalysts, requires characterization with complementary, correlated analysis techniques. An integrated setup has been developed to perform transmission electron microscopy (TEM) and single-molecule fluorescence (SMF) microscopy on such nanostructured samples. Correlated structure–reactivity information was obtained for 100 nm thin, microtomed sections of a ...

The q-dependent detrended cross-correlation analysis of stock market

Science.gov (United States)

Zhao, Longfeng; Li, Wei; Fenu, Andrea; Podobnik, Boris; Wang, Yougui; Stanley, H. Eugene

2018-02-01

Properties of the q-dependent cross-correlation matrices of the stock market have been analyzed by using random matrix theory and complex networks. The correlation structures of the fluctuations at different magnitudes have unique properties. The cross-correlations among small fluctuations are much stronger than those among large fluctuations. The large and small fluctuations are dominated by different groups of stocks. We use complex network representation to study these q-dependent matrices and discover some new identities. By utilizing those q-dependent correlation-based networks, we are able to construct some portfolios of those more independent stocks which consistently perform better. The optimal multifractal order for portfolio optimization is around q  =  2 under the mean-variance portfolio framework, and q\\in[2, 6] under the expected shortfall criterion. These results have deepened our understanding regarding the collective behavior of the complex financial system.

Structure of a financial cross-correlation matrix under attack

Science.gov (United States)

Lim, Gyuchang; Kim, SooYong; Kim, Junghwan; Kim, Pyungsoo; Kang, Yoonjong; Park, Sanghoon; Park, Inho; Park, Sang-Bum; Kim, Kyungsik

2009-09-01

We investigate the structure of a perturbed stock market in terms of correlation matrices. For the purpose of perturbing a stock market, two distinct methods are used, namely local and global perturbation. The former involves replacing a correlation coefficient of the cross-correlation matrix with one calculated from two Gaussian-distributed time series while the latter reconstructs the cross-correlation matrix just after replacing the original return series with Gaussian-distributed time series. Concerning the local case, it is a technical study only and there is no attempt to model reality. The term ‘global’ means the overall effect of the replacement on other untouched returns. Through statistical analyses such as random matrix theory (RMT), network theory, and the correlation coefficient distributions, we show that the global structure of a stock market is vulnerable to perturbation. However, apart from in the analysis of inverse participation ratios (IPRs), the vulnerability becomes dull under a small-scale perturbation. This means that these analysis tools are inappropriate for monitoring the whole stock market due to the low sensitivity of a stock market to a small-scale perturbation. In contrast, when going down to the structure of business sectors, we confirm that correlation-based business sectors are regrouped in terms of IPRs. This result gives a clue about monitoring the effect of hidden intentions, which are revealed via portfolios taken mostly by large investors.

FLUORESCENCE PROBING OF THE FORMATION OF HYDROPHOBIC MICRODOMAINS BY CROSS-LINKED POLY(ALKYLMETHYLDIALLYLAMMONIUM BROMIDES) IN AQUEOUS-SOLUTION

NARCIS (Netherlands)

WANG, GJ; ENGBERTS, J B F N

Pyrene has been used as a fluorescence probe to investigate the conformational behavior of cross-linked poly(alkylmethyldiallylammonium bromides) in aqueous solutions. Binding of pyrene to hydrophobic microdomains, formed by the polysoaps, is reflected by a change in the ratio I-1/I-3 of the

Diversity and Ecological Correlates of Red Fluorescence in Marine Fishes

Directory of Open Access Journals (Sweden)

Nils Anthes

2016-11-01

Full Text Available Marine environments at depths below -10 to -25 m are almost devoid of ambient red sunlight because water quickly attenuates long wavelengths. This stenospectral light environment presents unique opportunities for organisms that can transform ambient blue-green light into red light by fluorescence. Numerous marine fish species display intricate patterns of fluorescence. Because color vision is a key component of fish sensory ecology, several putative visual functions of red fluorescence have been proposed but are difficult to test experimentally. Here, we follow a comparative approach to assess the consistency between the phylogenetic distribution of red fluorescence with its presumed functions. We collected and analyzed the largest data set of red fluorescence in fishes to date, consisting of confirmed cases in 272 primarily diurnal fish species from 49 out of 90 surveyed fish families and 12 out of 21 surveyed fish orders, contrasted to 393 fish species with confirmed absence of red fluorescence. Based on a priori hypotheses on adaptive function, we compare the prevalence of red fluorescence among pre-defined sets of species based on ecological or biological characteristics while controlling for shared ancestry. When comparing between species, we find no evidence that red fluorescence is more prevalent in deep-water species, contrasting with our recent finding that fluorescence brightness increases with depth within species. There is also no evidence for a role in group-driven communication. Phylogenetic patterns are consistent, however, with three other predictions. First, fluorescence with a rather patchy distribution across the body occurred significantly more often among sit-and-wait predators or otherwise sedentary fish than in more mobile species, consistent with background matching for camouflage. Second, small, predatory fishes tended to show red fluorescent irides disproportionally often consistent with a proposed function in prey

Two-focus fluorescence correlation spectroscopy

International Nuclear Information System (INIS)

Dertinger, T.

2007-05-01

Fluorescence Correlation Spectroscopy (FCS) has been invented more than 30 years ago and experienced a renaissance after stable and affordable laser sources and low-noise single-photon detectors have become available. Its ability to measure diffusion coefficients at nanomolar concentrations of analyte made it a widely used tool in biophysics. However, in recent years it has been shown by many authors that aberrational (e.g. astigmatism) and photophysical effects (e.g. optical saturation) may influence the result of an FCS experiment dramatically, so that a precise and reliable estimation of the diffusion coefficient is no longer possible. In this thesis, we report on the development, implementation, and application of a new and robust modification of FCS that we termed two-focus FCS (2fFCS) and which fulfils two requirements: (i) It introduces an external ruler into the measurement by generating two overlapping laser foci of precisely known and fixed distance. (ii) These two foci and corresponding detection regions are generated in such a way that the corresponding molecule detection functions (MDFs) are sufficiently well described by a simple two-parameter model yielding accurate diffusion coefficients when applied to 2fFCS data analysis. Both these properties enable us to measure absolute values of the diffusion coefficient with an accuracy of a few percent. Moreover, it will turn out that the new technique is robust against refractive index mismatch, coverslide thickness deviations, and optical saturation effects, which so often trouble conventional FCS measurements. This thesis deals mainly with the introduction of the new measurement scheme, 2fFCS, but also presents several applications with far-reaching importance. (orig.)

Two-focus fluorescence correlation spectroscopy

Energy Technology Data Exchange (ETDEWEB)

Dertinger, T.

2007-05-15

Fluorescence Correlation Spectroscopy (FCS) has been invented more than 30 years ago and experienced a renaissance after stable and affordable laser sources and low-noise single-photon detectors have become available. Its ability to measure diffusion coefficients at nanomolar concentrations of analyte made it a widely used tool in biophysics. However, in recent years it has been shown by many authors that aberrational (e.g. astigmatism) and photophysical effects (e.g. optical saturation) may influence the result of an FCS experiment dramatically, so that a precise and reliable estimation of the diffusion coefficient is no longer possible. In this thesis, we report on the development, implementation, and application of a new and robust modification of FCS that we termed two-focus FCS (2fFCS) and which fulfils two requirements: (i) It introduces an external ruler into the measurement by generating two overlapping laser foci of precisely known and fixed distance. (ii) These two foci and corresponding detection regions are generated in such a way that the corresponding molecule detection functions (MDFs) are sufficiently well described by a simple two-parameter model yielding accurate diffusion coefficients when applied to 2fFCS data analysis. Both these properties enable us to measure absolute values of the diffusion coefficient with an accuracy of a few percent. Moreover, it will turn out that the new technique is robust against refractive index mismatch, coverslide thickness deviations, and optical saturation effects, which so often trouble conventional FCS measurements. This thesis deals mainly with the introduction of the new measurement scheme, 2fFCS, but also presents several applications with far-reaching importance. (orig.)

CROSS-CORRELATION MODELLING OF SURFACE WATER – GROUNDWATER INTERACTION USING THE EXCEL SPREADSHEET APPLICATION

Directory of Open Access Journals (Sweden)

Kristijan Posavec

2017-01-01

Full Text Available Modelling responses of groundwater levels in aquifer systems, which occur as a reaction to changes in aquifer system boundary conditions such as river or stream stages, is commonly being studied using statistical methods, namely correlation, cross-correlation and regression methods. Although correlation and regression analysis tools are readily available in Microsoft Excel, a widely applied spreadsheet industry standard, the cross-correlation analysis tool is missing. As a part of research of groundwater pressure propagation into alluvial aquifer systems of the Sava and Drava/Danube River catchments following river stages rise, focused on estimating groundwater pressure travel times in aquifers, an Excel spreadsheet data analysis application for cross-correlation modelling has been designed and used in modelling surface water – groundwater interaction. Examples of fi eld data from the Zagreb aquifer system and the Kopački rit Nature Park aquifer system are used to illustrate the usefulness of the cross-correlation application.

Cross-correlations in volume space: Differences between buy and sell volumes

Science.gov (United States)

Lee, Sun Young; Hwang, Dong Il; Kim, Min Jae; Koh, In Gyu; Kim, Soo Yong

2011-03-01

We study the cross-correlations of buy and sell volumes on the Korean stock market in high frequency. We observe that the pulling effects of volumes are as small as that of returns. The properties of the correlations of buy and sell volumes differ. They are explained by the degree of synchronization of stock volumes. Further, the pulling effects on the minimal spanning tree are studied. In minimal spanning trees with directed links, the large pulling effects are clustered at the center, not uniformly distributed. The Epps effect of buy and sell volumes are observed. The reversal of the cross-correlations of buy and sell volumes is also detected.

Study of water flowrate using time transient and cross-correlation techniques with 82Br radiotracer

International Nuclear Information System (INIS)

Salgado, William L.; Brandao, Luiz E.B.

2013-01-01

This paper aims to determinate the water flowrate using Time Transient and Cross-Correlation techniques. The detection system uses two NaI (T1) detectors adequately positioned on the outside of pipe and a gamma-ray source ( 82 Br radiotracer). The water flowrate measurements using Time Transient and Cross-Correlation techniques were compared to invasive conventional measurements of the flowrate previously installed in pipeline. Discrepancies between Time Transient and Cross-Correlation techniques flowmeter previously installed in pipeline. Discrepancies between Time Transient and Cross-Correlation techniques flowrate values were found to be less than 3% in relation to conventional ones. (author)

Autocorrelation and cross-correlation in time series of homicide and attempted homicide

Science.gov (United States)

Machado Filho, A.; da Silva, M. F.; Zebende, G. F.

2014-04-01

We propose in this paper to establish the relationship between homicides and attempted homicides by a non-stationary time-series analysis. This analysis will be carried out by Detrended Fluctuation Analysis (DFA), Detrended Cross-Correlation Analysis (DCCA), and DCCA cross-correlation coefficient, ρ(n). Through this analysis we can identify a positive cross-correlation between homicides and attempted homicides. At the same time, looked at from the point of view of autocorrelation (DFA), this analysis can be more informative depending on time scale. For short scale (days), we cannot identify auto-correlations, on the scale of weeks DFA presents anti-persistent behavior, and for long time scales (n>90 days) DFA presents a persistent behavior. Finally, the application of this new type of statistical analysis proved to be efficient and, in this sense, this paper can contribute to a more accurate descriptive statistics of crime.

World currency exchange rate cross-correlations

Science.gov (United States)

Droå¼dż, S.; Górski, A. Z.; Kwapień, J.

2007-08-01

World currency network constitutes one of the most complex structures that is associated with the contemporary civilization. On a way towards quantifying its characteristics we study the cross correlations in changes of the daily foreign exchange rates within the basket of 60 currencies in the period December 1998 May 2005. Such a dynamics turns out to predominantly involve one outstanding eigenvalue of the correlation matrix. The magnitude of this eigenvalue depends however crucially on which currency is used as a base currency for the remaining ones. Most prominent it looks from the perspective of a peripheral currency. This largest eigenvalue is seen to systematically decrease and thus the structure of correlations becomes more heterogeneous, when more significant currencies are used as reference. An extreme case in this later respect is the USD in the period considered. Besides providing further insight into subtle nature of complexity, these observations point to a formal procedure that in general can be used for practical purposes of measuring the relative currencies significance on various time horizons.

Audio Quality Assurance : An Application of Cross Correlation

DEFF Research Database (Denmark)

Jurik, Bolette Ammitzbøll; Nielsen, Jesper Asbjørn Sindahl

2012-01-01

We describe algorithms for automated quality assurance on content of audio files in context of preservation actions and access. The algorithms use cross correlation to compare the sound waves. They are used to do overlap analysis in an access scenario, where preserved radio broadcasts are used in...

Nonreciprocal Green’s function retrieval by cross correlation

NARCIS (Netherlands)

Wapenaar, C.P.A.

2006-01-01

The cross correlation of two recordings of a diffuse acoustic wave field at different receivers yields the Green’s function between these receivers. In nearly all cases considered so far the wave equation obeys time-reversal invariance and the Green’s function obeys source-receiver reciprocity. Here

Iodine imaging in thyroid by fluorescent X-ray CT with 0.05 mm spatial resolution

Energy Technology Data Exchange (ETDEWEB)

Takeda, T. E-mail: ttakeda@md.tsukuba.ac.jp; Yu, Q.; Yashiro, T.; Zeniya, T.; Wu, J.; Hasegawa, Y.; Thet Thet Lwin; Hyodo, K.; Yuasa, T.; Dilmanian, F.A.; Akatsuka, T.; Itai, Y

2001-07-21

Fluorescent X-ray computed tomography (FXCT) at a 0.05 mm in-plane spatial resolution and 0.05 mm slice thickness depicted the cross sectional distribution of endogenous iodine within thyroid. The distribution obtained from the FXCT image correlated closely to that obtained from the pathological pictures.

CROSS-CORRELATION BETWEEN X-RAY AND OPTICAL/NEAR-INFRARED BACKGROUND INTENSITY FLUCTUATIONS

Energy Technology Data Exchange (ETDEWEB)

Mitchell-Wynne, Ketron; Cooray, Asantha [Department of Physics and Astronomy, University of California, Irvine, CA 92697 (United States); Xue, Yongquan [CAS Key Laboratory for Researches in Galaxies and Cosmology, Center for Astrophysics, Department of Astronomy, University of Science and Technology of China, Chinese Academy of Sciences, Hefei, Anhui 230026 (China); Luo, Bin [School of Astronomy and Space Science, Nanjing University, Nanjing, 210093 (China); Brandt, William [Department of Astronomy and Astrophysics, Pennsylvania State University, University Park, PA, 16802 (United States); Koekemoer, Anton [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States)

2016-12-01

Angular power spectra of optical and infrared background anisotropies at wavelengths between 0.5 and 5 μ m are a useful probe of faint sources present during reionization, in addition to faint galaxies and diffuse signals at low redshift. The cross-correlation of these fluctuations with backgrounds at other wavelengths can be used to separate some of these signals. A previous study on the cross-correlation between X-ray and Spitzer fluctuations at 3.6 μ m and 4.5 μ m has been interpreted as evidence for direct collapse black holes present at z  > 12. Here we return to this cross-correlation and study its wavelength dependence from 0.5 to 4.5 μ m using Hubble and Spitzer data in combination with a subset of the 4 Ms Chandra observations in GOODS-S/ECDFS. Our study involves five Hubble bands at 0.6, 0.7, 0.85, 1.25, and 1.6 μ m, and two Spitzer -IRAC bands at 3.6 μ m and 4.5 μ m. We confirm the previously seen cross-correlation between 3.6 μ m (4.5 μ m) and X-rays with 3.7 σ (4.2 σ ) and 2.7 σ (3.7 σ ) detections in the soft [0.5–2] keV and hard [2–8] keV X-ray bands, respectively, at angular scales above 20 arcsec. The cross-correlation of X-rays with Hubble is largely anticorrelated, ranging between the levels of 1.4 σ –3.5 σ for all the Hubble and X-ray bands. This lack of correlation in the shorter optical/NIR bands implies the sources responsible for the cosmic infrared background at 3.6 and 4.5 μ m are at least partly dissimilar to those at 1.6 μ m and shorter.

Corrections for frequency domain transformations of Winfrith binary cross correlator responses

International Nuclear Information System (INIS)

Cummins, J.D.

1968-04-01

This report considers the corrections for frequency domain transformations of Winfrith binary cross correlator responses; (i) for the finite bandwidth of the equivalent input signal; (2) for the finite time required for the actuator to move between the two positions appropriate to the two levels of the periodic binary chain code input and (3) for the averaging of experimental determinations of the system frequency response and calculations of the standard deviations of the modulus and phase of the frequency responses determined from the cross correlator responses. (author)

A fluorescence scanning electron microscope

International Nuclear Information System (INIS)

Kanemaru, Takaaki; Hirata, Kazuho; Takasu, Shin-ichi; Isobe, Shin-ichiro; Mizuki, Keiji; Mataka, Shuntaro; Nakamura, Kei-ichiro

2009-01-01

Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e. a fluorescence microscope (FM) and an electron microscope (EM). In the current study, a scanning electron microscope (SEM) (JEOL JXA8600 M) was combined with a fluorescence digital camera microscope unit and this hybrid instrument was named a fluorescence SEM (FL-SEM). In the labeling of FL-SEM samples, both Fluolid, which is an organic EL dye, and Alexa Fluor, were employed. We successfully demonstrated that the FL-SEM is a simple and practical tool for correlative fluorescence and electron microscopy.

The Effect of a Fluorophore Photo-Physics on the Lipid Vesicle Diffusion Coefficient Studied by Fluorescence Correlation Spectroscopy.

Science.gov (United States)

Drabik, Dominik; Przybyło, Magda; Sikorski, Aleksander; Langner, Marek

2016-03-01

Fluorescence Correlation Spectroscopy (FCS) is a technique, which allows determination of the diffusion coefficient and concentration of fluorescent objects suspended in the solution. The measured parameter is the fluctuation of the fluorescence signal emitted by diffusing molecules. When 100 nm DOPC vesicles labeled with various fluorescent dyes (Fluorescein-PE, NBD-PE, Atto488 DOPE or βBodipy FL) were measured, different values of diffusion coefficients have been obtained. These diffusion coefficients were different from the expected values measured using the dynamic light scattering method (DLS). The FCS was initially developed for solutions containing small fluorescent molecules therefore the observed inconsistency may result from the nature of vesicle suspension itself. The duration of the fluorescence signal may depend on the following factors: the exposure time of the labeled object to the excitation beam, the photo-physical properties (e.g., stability) of a fluorophore, the theoretical model used for the calculations of the diffusion coefficient and optical properties of the vesicle suspension. The diffusion coefficients determined for differently labeled liposomes show that its dependence on vesicle size and quantity of fluorescent probed used for labeling was significant demonstrating that the fluorescence properties of the fluorophore itself (bleaching and/or blinking) were critical factors for a correct outcome of FCS experiment. The new, based on combined FCS and DLS measurements, method for the determination of the focal volume prove itself to be useful for the evaluation of a fluorescence dye with respect to its applicability for FCS experiment.

Perspectives of Cross-Correlation in Seismic Monitoring at the International Data Centre

Science.gov (United States)

Bobrov, Dmitry; Kitov, Ivan; Zerbo, Lassina

2014-03-01

We demonstrate that several techniques based on waveform cross-correlation are able to significantly reduce the detection threshold of seismic sources worldwide and to improve the reliability of arrivals by a more accurate estimation of their defining parameters. A master event and the events it can find using waveform cross-correlation at array stations of the International Monitoring System (IMS) have to be close. For the purposes of the International Data Centre (IDC), one can use the spatial closeness of the master and slave events in order to construct a new automatic processing pipeline: all qualified arrivals detected using cross-correlation are associated with events matching the current IDC event definition criteria (EDC) in a local association procedure. Considering the repeating character of global seismicity, more than 90 % of events in the reviewed event bulletin (REB) can be built in this automatic processing. Due to the reduced detection threshold, waveform cross-correlation may increase the number of valid REB events by a factor of 1.5-2.0. Therefore, the new pipeline may produce a more comprehensive bulletin than the current pipeline—the goal of seismic monitoring. The analysts' experience with the cross correlation event list (XSEL) shows that the workload of interactive processing might be reduced by a factor of two or even more. Since cross-correlation produces a comprehensive list of detections for a given master event, no additional arrivals from primary stations are expected to be associated with the XSEL events. The number of false alarms, relative to the number of events rejected from the standard event list 3 (SEL3) in the current interactive processing—can also be reduced by the use of several powerful filters. The principal filter is the difference between the arrival times of the master and newly built events at three or more primary stations, which should lie in a narrow range of a few seconds. In this study, one event at a

Positive Noise Cross Correlation in a Copper Pair Splitter.

Science.gov (United States)

Das, Anindya; Ronen, Yuval; Heiblum, Moty; Shtrikman, Hadas; Mahalu, Diana

2012-02-01

Entanglement is in heart of the Einstein-Podolsky-Rosen (EPR) paradox, in which non-locality is a fundamental property. Up to date spin entanglement of electrons had not been demonstrated. Here, we provide direct evidence of such entanglement by measuring: non-local positive current correlation and positive cross correlation among current fluctuations, both of separated electrons born by a Cooper-pair-beam-splitter. The realization of the splitter is provided by injecting current from an Al superconductor contact into two, single channel, pure InAs nanowires - each intercepted by a Coulomb blockaded quantum dot (QD). The QDs impedes strongly the flow of Cooper pairs allowing easy single electron transport. The passage of electron in one wire enables the simultaneous passage of the other in the neighboring wire. The splitting efficiency of the Cooper pairs (relative to Cooper pairs actual current) was found to be ˜ 40%. The positive cross-correlations in the currents and their fluctuations (shot noise) are fully consistent with entangled electrons produced by the beam splitter.

Exploring the Dynamics of Cell Processes through Simulations of Fluorescence Microscopy Experiments

Science.gov (United States)

Angiolini, Juan; Plachta, Nicolas; Mocskos, Esteban; Levi, Valeria

2015-01-01

Fluorescence correlation spectroscopy (FCS) methods are powerful tools for unveiling the dynamical organization of cells. For simple cases, such as molecules passively moving in a homogeneous media, FCS analysis yields analytical functions that can be fitted to the experimental data to recover the phenomenological rate parameters. Unfortunately, many dynamical processes in cells do not follow these simple models, and in many instances it is not possible to obtain an analytical function through a theoretical analysis of a more complex model. In such cases, experimental analysis can be combined with Monte Carlo simulations to aid in interpretation of the data. In response to this need, we developed a method called FERNET (Fluorescence Emission Recipes and Numerical routines Toolkit) based on Monte Carlo simulations and the MCell-Blender platform, which was designed to treat the reaction-diffusion problem under realistic scenarios. This method enables us to set complex geometries of the simulation space, distribute molecules among different compartments, and define interspecies reactions with selected kinetic constants, diffusion coefficients, and species brightness. We apply this method to simulate single- and multiple-point FCS, photon-counting histogram analysis, raster image correlation spectroscopy, and two-color fluorescence cross-correlation spectroscopy. We believe that this new program could be very useful for predicting and understanding the output of fluorescence microscopy experiments. PMID:26039162

L X-ray fluorescence cross sections experimentally determined for elements with 45

Energy Technology Data Exchange (ETDEWEB)

Bonzi, Edgardo V., E-mail: bonzie@famaf.unc.edu.ar [Facultad de Matematica, Astronomia y Fisica, Universidad Nacional de Cordoba, Ciudad Universitaria-5010, Cordoba (Argentina); Badiger, Nagappa M. [Departments of Physics, Karnatak University, Dharwad 580 003, Karnataka (India); Grad, Gabriela B. [Facultad de Matematica, Astronomia y Fisica, Universidad Nacional de Cordoba, Ciudad Universitaria-5010, Cordoba (Argentina); Barrea, Raul A. [The Biophysics Collaborative Access Team (BioCAT), Dept of Biological Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago, IL 60616 (United States); Figueroa, Rodolo G. [Departamento de Cs. Fisicas, Universidad de La Frontera, Temuco (Chile)

2012-04-15

Experimental determination of L fluorescence cross-sections for elements with 45cross sections obtained in this work were compared with data calculated using coefficients from , , and Scofield and Puri et al. (1993, 1995) - Highlights: Black-Right-Pointing-Pointer Experimental data of L fluorescence cross-sections with 45cross sections Ll, L{alpha}, L{beta}{sub I}, L{beta}{sub II}, L{gamma}{sub {Iota}} and L{gamma}{sub {Iota}{Iota}} obtained, were compared with calculated data. Black-Right-Pointing-Pointer The Hypermet function was used to fit the data, because it considers a tail on the left side of the peak. Black-Right-Pointing-Pointer The tail is relevant when a small peak has another one on the right side with a big area.

Fluorescence Spectroscopy Applied to Monitoring Biodiesel Degradation: Correlation with Acid Value and UV Absorption Analyses.

Science.gov (United States)

Vasconcelos, Maydla Dos Santos; Passos, Wilson Espíndola; Lescanos, Caroline Honaiser; Pires de Oliveira, Ivan; Trindade, Magno Aparecido Gonçalves; Caires, Anderson Rodrigues Lima; Muzzi, Rozanna Marques

2018-01-01

The techniques used to monitor the quality of the biodiesel are intensely discussed in the literature, partly because of the different oil sources and their intrinsic physicochemical characteristics. This study aimed to monitor the thermal degradation of the fatty acid methyl esters of Sesamum indicum L. and Raphanus sativus L. biodiesels (SILB and RSLB, resp.). The results showed that both biodiesels present a high content of unsaturated fatty acids, ∼84% (SILB) and ∼90% (RSLB). The SILB had a high content of polyunsaturated linoleic fatty acid (18  :  2), about 49%, and the oleic monounsaturated (18  :  1), ∼34%. On the other hand, RSLB presented a considerable content of linolenic fatty acid (18  :  3), ∼11%. The biodiesel samples were thermal degraded at 110°C for 48 hours, and acid value, UV absorption, and fluorescence spectroscopy analysis were carried out. The results revealed that both absorption and fluorescence presented a correlation with acid value as a function of degradation time by monitoring absorptions at 232 and 270 nm as well as the emission at 424 nm. Although the obtained correlation is not completely linear, a direct correlation was observed in both cases, revealing that both properties can be potentially used for monitoring the biodiesel degradation.

Fluorescence Spectroscopy Applied to Monitoring Biodiesel Degradation: Correlation with Acid Value and UV Absorption Analyses

Directory of Open Access Journals (Sweden)

Maydla dos Santos Vasconcelos

2018-01-01

Full Text Available The techniques used to monitor the quality of the biodiesel are intensely discussed in the literature, partly because of the different oil sources and their intrinsic physicochemical characteristics. This study aimed to monitor the thermal degradation of the fatty acid methyl esters of Sesamum indicum L. and Raphanus sativus L. biodiesels (SILB and RSLB, resp.. The results showed that both biodiesels present a high content of unsaturated fatty acids, ∼84% (SILB and ∼90% (RSLB. The SILB had a high content of polyunsaturated linoleic fatty acid (18  :  2, about 49%, and the oleic monounsaturated (18  :  1, ∼34%. On the other hand, RSLB presented a considerable content of linolenic fatty acid (18  :  3, ∼11%. The biodiesel samples were thermal degraded at 110°C for 48 hours, and acid value, UV absorption, and fluorescence spectroscopy analysis were carried out. The results revealed that both absorption and fluorescence presented a correlation with acid value as a function of degradation time by monitoring absorptions at 232 and 270 nm as well as the emission at 424 nm. Although the obtained correlation is not completely linear, a direct correlation was observed in both cases, revealing that both properties can be potentially used for monitoring the biodiesel degradation.

Scanning cross-correlator for monitoring uniform 3D ellipsoidal laser beams

CERN Document Server

Zelenogorskii, V V; Gacheva, E I; Gelikonov, G V; Krasilnikov, M; Mart'yanov, M A; Mironov, S Yu; Potemkin, A K; Syresin, E M; Stephan, F; Khazanov, E A

2014-01-01

The specific features of experimental implementation of a cross-correlator with a scan rate above 1600 cm s(-1) and a spatial delay amplitude of more than 15 mm are considered. The possibility of measuring the width of femtosecond pulses propagating in a train 300 mu s in duration with a repetition rate of 1 MHz is demonstrated. A time resolution of 300 fs for the maximum time window of 50 ps is attained.The cross-correlator is aimed at testing 3D pulses of a laser driver of an electron photo-injector.

Fluorescence Correlation Spectroscopy to Study Diffusion of Polymer Chains within Layered Hydrogen-Bonded Polymer Films

Science.gov (United States)

Pristinski, Denis; Kharlampieva, Evguenia; Sukhishvili, Svetlana

2002-03-01

Fluorescence Correlation Spectroscopy (FCS) has been used to probe molecular motions within polymer multilayers formed by hydrogen-bonding sequential self-assembly. Polyethylene glycol (PEG) molecules were end-labeled with the fluorescent tags, and self-assembled with polymethacrylic acid (PMAA) using layer-by-layer deposition. We have found that molecules included in the top adsorbed layer have significant mobility at the millisecond time scale, probably due to translational diffusion. However, their dynamics deviate from classical Brownian motion with a single diffusion time. Possible reasons for the deviation are discussed. We found that motions were significantly slowed with increasing depth within the PEG/PMAA multilayer. This phenomena occured in a narrow pH range around 4.0 in which intermolecular interactions were relatively weak.

Nonlinear Analysis on Cross-Correlation of Financial Time Series by Continuum Percolation System

Science.gov (United States)

Niu, Hongli; Wang, Jun

We establish a financial price process by continuum percolation system, in which we attribute price fluctuations to the investors’ attitudes towards the financial market, and consider the clusters in continuum percolation as the investors share the same investment opinion. We investigate the cross-correlations in two return time series, and analyze the multifractal behaviors in this relationship. Further, we study the corresponding behaviors for the real stock indexes of SSE and HSI as well as the liquid stocks pair of SPD and PAB by comparison. To quantify the multifractality in cross-correlation relationship, we employ multifractal detrended cross-correlation analysis method to perform an empirical research for the simulation data and the real markets data.

Cross-correlation of weak lensing and gamma rays: implications for the nature of dark matter

Science.gov (United States)

Tröster, Tilman; Camera, Stefano; Fornasa, Mattia; Regis, Marco; van Waerbeke, Ludovic; Harnois-Déraps, Joachim; Ando, Shin'ichiro; Bilicki, Maciej; Erben, Thomas; Fornengo, Nicolao; Heymans, Catherine; Hildebrandt, Hendrik; Hoekstra, Henk; Kuijken, Konrad; Viola, Massimo

2017-05-01

We measure the cross-correlation between Fermi gamma-ray photons and over 1000 deg2 of weak lensing data from the Canada-France-Hawaii Telescope Lensing Survey (CFHTLenS), the Red Cluster Sequence Lensing Survey (RCSLenS), and the Kilo Degree Survey (KiDS). We present the first measurement of tomographic weak lensing cross-correlations and the first application of spectral binning to cross-correlations between gamma rays and weak lensing. The measurements are performed using an angular power spectrum estimator while the covariance is estimated using an analytical prescription. We verify the accuracy of our covariance estimate by comparing it to two internal covariance estimators. Based on the non-detection of a cross-correlation signal, we derive constraints on weakly interacting massive particle (WIMP) dark matter. We compute exclusion limits on the dark matter annihilation cross-section , decay rate Γdec and particle mass mDM. We find that in the absence of a cross-correlation signal, tomography does not significantly improve the constraining power of the analysis. Assuming a strong contribution to the gamma-ray flux due to small-scale clustering of dark matter and accounting for known astrophysical sources of gamma rays, we exclude the thermal relic cross-section for particle masses of mDM ≲ 20 GeV.

Detecting Near-Earth Objects Using Cross-Correlation with a Point Spread Function

Science.gov (United States)

2009-03-01

impact in the Yucatan Peninsula caused the extinction of the dinosaurs in the Cretaceous Period [Fix, 1995]. Even the Moon is pot marked by many...the atmosphere that the light traverses. For this reason , it is typically better to be at higher elevations to decrease the amount of atmosphere the...detection on average for the Rayleigh sampling with cross-correlation of a PSF than the Rayleigh sampling without cross- correlation. For this reason

Spatially varying cross-correlation coefficients in the presence of nugget effects

KAUST Repository

Kleiber, William; Genton, Marc G.

2012-01-01

We derive sufficient conditions for the cross-correlation coefficient of a multivariate spatial process to vary with location when the spatial model is augmented with nugget effects. The derived class is valid for any choice of covariance functions, and yields substantial flexibility between multiple processes. The key is to identify the cross-correlation coefficient matrix with a contraction matrix, which can be either diagonal, implying a parsimonious formulation, or a fully general contraction matrix, yielding greater flexibility but added model complexity. We illustrate the approach with a bivariate minimum and maximum temperature dataset in Colorado, allowing the two variables to be positively correlated at low elevations and nearly independent at high elevations, while still yielding a positive definite covariance matrix. © 2012 Biometrika Trust.

Spatially varying cross-correlation coefficients in the presence of nugget effects

KAUST Repository

Kleiber, William

2012-11-29

We derive sufficient conditions for the cross-correlation coefficient of a multivariate spatial process to vary with location when the spatial model is augmented with nugget effects. The derived class is valid for any choice of covariance functions, and yields substantial flexibility between multiple processes. The key is to identify the cross-correlation coefficient matrix with a contraction matrix, which can be either diagonal, implying a parsimonious formulation, or a fully general contraction matrix, yielding greater flexibility but added model complexity. We illustrate the approach with a bivariate minimum and maximum temperature dataset in Colorado, allowing the two variables to be positively correlated at low elevations and nearly independent at high elevations, while still yielding a positive definite covariance matrix. © 2012 Biometrika Trust.

Detection of rhodopsin dimerization in situ by PIE-FCCS, a time-resolved fluorescence spectroscopy.

Science.gov (United States)

Smith, Adam W

2015-01-01

Rhodopsin self-associates in the plasma membrane. At low concentrations, the interactions are consistent with a monomer-dimer equilibrium (Comar et al., J Am Chem Soc 136(23):8342-8349, 2014). At high concentrations in native tissue, higher-order clusters have been observed (Fotiadis et al., Nature 421:127-128, 2003). The physiological role of rhodopsin dimerization is still being investigated, but it is clear that a quantitative assessment is essential to determining the function of rhodopsin clusters in vision. To quantify rhodopsin interactions, I will outline the theory and methodology of a specialized time-resolved fluorescence spectroscopy for measuring membrane protein-protein interactions called pulsed-interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCCS). The strength of this technique is its ability to quantify rhodopsin interactions in situ (i.e., a live cell plasma membrane). There are two reasons for restricting the scope to live cell membranes. First, the compositional heterogeneity of the plasma membrane creates a complex milieu with thousands of lipid, protein, and carbohydrate species. This makes it difficult to infer quaternary interactions from detergent solubilized samples or construct a model phospholipid bilayer that recapitulates all of the interactions present in native membranes. Second, organizational structure and dynamics is a key feature of the plasma membrane, and fixation techniques like formaldehyde cross-linking and vitrification will modulate the interactions. PIE-FCCS is based on two-color fluorescence imaging with time-correlated single-photon counting (TCSPC) (Becker et al., Rev Sci Instrum 70:1835-1841, 1999). By time-tagging every detected photon, the data can be analyzed as a fluorescence intensity distribution, fluorescence lifetime histogram, or fluorescence (cross-)correlation spectra (FCS/FCCS) (Becker, Advanced time-correlated single-photon counting techniques, Springer, Berlin, 2005). These

Power-law cross-correlations estimation under heavy tails

Czech Academy of Sciences Publication Activity Database

Krištoufek, Ladislav

2016-01-01

Roč. 40, č. 1 (2016), s. 163-172 ISSN 1007-5704 R&D Projects: GA ČR(CZ) GP14-11402P Institutional support: RVO:67985556 Keywords : Power-law cross-correlations * Heavy tails * Monte Carlo study Subject RIV: AH - Economics Impact factor: 2.784, year: 2016 http://library.utia.cas.cz/separaty/2016/E/kristoufek-0472030.pdf

Statistical analysis of correlated experimental data and neutron cross section evaluation

International Nuclear Information System (INIS)

Badikov, S.A.

1998-01-01

The technique for evaluation of neutron cross sections on the basis of statistical analysis of correlated experimental data is presented. The most important stages of evaluation beginning from compilation of correlation matrix for measurement uncertainties till representation of the analysis results in the ENDF-6 format are described in details. Special attention is paid to restrictions (positive uncertainty) on covariation matrix of approximate parameters uncertainties generated within the method of least square fit which is derived from physical reasons. The requirements for source experimental data assuring satisfaction of the restrictions mentioned above are formulated. Correlation matrices of measurement uncertainties in particular should be also positively determined. Variants of modelling the positively determined correlation matrices of measurement uncertainties in a situation when their consequent calculation on the basis of experimental information is impossible are discussed. The technique described is used for creating the new generation of estimates of dosimetric reactions cross sections for the first version of the Russian dosimetric file (including nontrivial covariation information)

Minimizing the trend effect on detrended cross-correlation analysis with empirical mode decomposition

International Nuclear Information System (INIS)

Zhao Xiaojun; Shang Pengjian; Zhao Chuang; Wang Jing; Tao Rui

2012-01-01

Highlights: ► Investigate the effects of linear, exponential and periodic trends on DCCA. ► Apply empirical mode decomposition to extract trend term. ► Strong and monotonic trends are successfully eliminated. ► Get the cross-correlation exponent in a persistent behavior without crossover. - Abstract: Detrended cross-correlation analysis (DCCA) is a scaling method commonly used to estimate long-range power law cross-correlation in non-stationary signals. However, the susceptibility of DCCA to trends makes the scaling results difficult to analyze due to spurious crossovers. We artificially generate long-range cross-correlated signals and systematically investigate the effect of linear, exponential and periodic trends. Specifically to the crossovers raised by trends, we apply empirical mode decomposition method which decomposes underlying signals into several intrinsic mode functions (IMF) and a residual trend. After the removal of residual term, strong and monotonic trends such as linear and exponential trends are successfully eliminated. But periodic trend cannot be separated out according to the criterion of IMF, which can be eliminated by Fourier transform. As a special case of DCCA, detrended fluctuation analysis presents similar results.

Cross correlations of quantum key distribution based on single-photon sources

International Nuclear Information System (INIS)

Dong Shuangli; Wang Xiaobo; Zhang Guofeng; Sun Jianhu; Zhang Fang; Xiao Liantuan; Jia Suotang

2009-01-01

We theoretically analyze the second-order correlation function in a quantum key distribution system with real single-photon sources. Based on single-event photon statistics, the influence of the modification caused by an eavesdropper's intervention and the effects of background signals on the cross correlations between authorized partners are presented. On this basis, we have shown a secure range of correlation against the intercept-resend attacks.

Cosmic microwave and infrared backgrounds cross-correlation for ISW detection

International Nuclear Information System (INIS)

Ilić, S

2014-01-01

We have investigated the cross-correlation between the cosmic infrared and microwave backgrounds (CIB and CMB) anisotropies through the integrated Sachs-Wolfe effect. We have first described the CIB anisotropies using a linearly biased power spectrum, then derive the theoretical angular power spectrum of the CMB-CIB cross-correlation for different instruments and frequencies. We have discussed the detectability of the ISW signal by performing a signal-to-noise (SNR) analysis with our predicted spectra. The significances obtained range from 6σ to 7σ in an ideal case, depending on the frequency; in realistic cases which account for the presence of noise including astrophysical contaminants, the results span the range 2 – 5σ, depending strongly on the major contribution to the noise term

The detection of T-Nos, a genetic element present in GMOs, by cross-priming isothermal amplification with real-time fluorescence.

Science.gov (United States)

Zhang, Fang; Wang, Liu; Fan, Kai; Wu, Jian; Ying, Yibin

2014-05-01

An isothermal cross-priming amplification (CPA) assay for Agrobacterium tumefaciens nopaline synthase terminator (T-Nos) was established and investigated in this work. A set of six specific primers, recognizing eight distinct regions on the T-Nos sequence, was designed. The CPA assay was performed at a constant temperature, 63 °C, and detected by real-time fluorescence. The results indicated that real-time fluorescent CPA had high specificity, and the limit of detection was 1.06 × 10(3) copies of rice genomic DNA, which could be detected in 40 min. Comparison of real-time fluorescent CPA and conventional polymerase chain reaction (PCR) was also performed. Results revealed that real-time fluorescent CPA had a comparable sensitivity to conventional real-time PCR and had taken a shorter time. In addition, different contents of genetically modified (GM)-contaminated rice seed powder samples were detected for practical application. The result showed real-time fluorescent CPA could detect 0.5 % GM-contaminated samples at least, and the whole reaction could be finished in 35 min. Real-time fluorescent CPA is sensitive enough to monitor labeling systems and provides an attractive method for the detection of GMO.

Measurement of correlated b quark cross sections at CDF

International Nuclear Information System (INIS)

Gerdes, D.

1994-09-01

Using data collected during the 1992--93 collider run at Fermilab, CDF has made measurements of correlated b quark cross section where one b is detected from a muon from semileptonic decay and the second b is detected with secondary vertex techniques. We report on measurements of the cross section as a function of the momentum of the second b and as a function of the azimuthal separation of the two b quarks, for transverse momentum of the initial b quark greater than 15 GeV. Results are compared to QCD predictions

A technique for plasma velocity-space cross-correlation

Science.gov (United States)

Mattingly, Sean; Skiff, Fred

2018-05-01

An advance in experimental plasma diagnostics is presented and used to make the first measurement of a plasma velocity-space cross-correlation matrix. The velocity space correlation function can detect collective fluctuations of plasmas through a localized measurement. An empirical decomposition, singular value decomposition, is applied to this Hermitian matrix in order to obtain the plasma fluctuation eigenmode structure on the ion distribution function. A basic theory is introduced and compared to the modes obtained by the experiment. A full characterization of these modes is left for future work, but an outline of this endeavor is provided. Finally, the requirements for this experimental technique in other plasma regimes are discussed.

Cross-correlation between EMG and center of gravity during quiet stance: theory and simulations.

Science.gov (United States)

Kohn, André Fabio

2005-11-01

Several signal processing tools have been employed in the experimental study of the postural control system in humans. Among them, the cross-correlation function has been used to analyze the time relationship between signals such as the electromyogram and the horizontal projection of the center of gravity. The common finding is that the electromyogram precedes the biomechanical signal, a result that has been interpreted in different ways, for example, the existence of feedforward control or the preponderance of a velocity feedback. It is shown here, analytically and by simulation, that the cross-correlation function is dependent in a complicated way on system parameters and on noise spectra. Results similar to those found experimentally, e.g., electromyogram preceding the biomechanical signal may be obtained in a postural control model without any feedforward control and without any velocity feedback. Therefore, correct interpretations of experimentally obtained cross-correlation functions may require additional information about the system. The results extend to other biomedical applications where two signals from a closed loop system are cross-correlated.

Multifractal detrended cross-correlation between the Chinese domestic and international gold markets based on DCCA and DMCA methods

Science.gov (United States)

Cao, Guangxi; Han, Yan; Chen, Yuemeng; Yang, Chunxia

2014-05-01

Based on the daily price data of Shanghai and London gold spot markets, we applied detrended cross-correlation analysis (DCCA) and detrended moving average cross-correlation analysis (DMCA) methods to quantify power-law cross-correlation between domestic and international gold markets. Results show that the cross-correlations between the Chinese domestic and international gold spot markets are multifractal. Furthermore, forward DMCA and backward DMCA seems to outperform DCCA and centered DMCA for short-range gold series, which confirms the comparison results of short-range artificial data in L. Y. He and S. P. Chen [Physica A 390 (2011) 3806-3814]. Finally, we analyzed the local multifractal characteristics of the cross-correlation between Chinese domestic and international gold markets. We show that multifractal characteristics of the cross-correlation between the Chinese domestic and international gold markets are time-varying and that multifractal characteristics were strengthened by the financial crisis in 2007-2008.

Cross-correlated imaging of distributed mode filtering rod fiber

DEFF Research Database (Denmark)

Laurila, Marko; Barankov, Roman; Jørgensen, Mette Marie

2013-01-01

We analyze the modal properties of an 85μm core distributed mode filtering rod fiber using cross-correlated (C2) imaging. We evaluate suppression of higher-order modes (HOMs) under severely misaligned mode excitation and identify a single-mode regime where HOMs are suppressed by more than 20dB....

Disparity in formulations used for fluorescent X-ray intensity measurements

International Nuclear Information System (INIS)

Mittal, Raj; Gupta, Sheenu

2011-01-01

The paper presents a problem in computations of X-ray fluorescence cross-sections, shell/sub-shell fluorescence yields, Coster-Kronig yields, vacancy alignment, etc. from X-ray fluorescence (XRF) studies. While using barn/atom as a unit for cross-sections if the atomic masses are not considered it causes a discrepancy in the measured cross-section, yield and alignment values. Most of the earlier publications are being quoted where such an oversight has occurred and discrepancy is evident. - Highlights: → Manuscript gives basic formulation for measurements of fluorescent X-ray intensities. → Most publications ignored the fact that use of barn/atom units for cross-sections requires atomic masses. → Published experimental results higher by a factor ≥2 or less by factor 1/M K . → Inspection of published data on XRF parameters needed.

Pilot Assessment of the Repeatability of Indocyanine Green Fluorescence Imaging and Correlation with Traditional Foot Perfusion Assessments.

Science.gov (United States)

Venermo, M; Settembre, N; Albäck, A; Vikatmaa, P; Aho, P-S; Lepäntalo, M; Inoue, Y; Terasaki, H

2016-10-01

Ankle brachial index (ABI), toe pressures (TP), and transcutaneous oxygen pressure (TcPO 2 ) are traditionally used in the assessment of critical limb ischemia (CLI). Indocyanine green (ICG) fluorescence imaging can be used to evaluate local circulation in the foot and to evaluate the severity of ischemia. This prospective study analyzed the suitability of a fluorescence imaging system (photodynamic eye [PDE]) in CLI. Forty-one patients with CLI were included. Of the patients, 66% had diabetes and there was an ischemic tissue lesion in 70% of the limbs. ABI, toe pressures, TcPO 2 and ICG-fluorescence imaging (ICG-FI) were measured in each leg. To study the repeatability of the ICG-FI, each patient underwent the study twice. After the procedure, foot circulation was measured using a time-intensity curve, where T1/2 (the time needed to achieve half of the maximum fluorescence intensity) and PDE10 (increase of the intensity during the first 10 s) were determined. A time-intensity curve was plotted using the same areas as for the TcPO 2 probes (n=123). The mean ABI was 0.43, TP 21 mmHg, TcPO 2 23 mmHg, T1/2 38 s, and PDE10 19 AU. Time-intensity curves were repeatable. In a Bland-Altman scatter plot, the 95% limits of agreement of PDE10 was 9.9 AU and the corresponding value of T1/2 was 14 s. Correlation between ABI and TP was significant (R=.73, p<.001), and it was weaker in diabetic patients (R=.47, p=.048) compared with non-diabetic patients (R=.89, p=.002). Correlations between ABI and TcPO 2 and TP and TcPO 2 were weak (R=.37, p=.05 and R=.43, p=.037, respectively). Correlation between TcPO 2 and PDE10 was strong in diabetic patients (R=.70, p=.003). According to this pilot study, ICG-FI with PDE can be used in the assessment of blood supply in the ischemic foot. Copyright © 2016 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

Using fluorescence correlation spectroscopy to study conformational changes in denatured proteins.

Science.gov (United States)

Sherman, Eilon; Itkin, Anna; Kuttner, Yosef Yehuda; Rhoades, Elizabeth; Amir, Dan; Haas, Elisha; Haran, Gilad

2008-06-01

Fluorescence correlation spectroscopy (FCS) is a sensitive analytical tool that allows dynamics and hydrodynamics of biomolecules to be studied under a broad range of experimental conditions. One application of FCS of current interest is the determination of the size of protein molecules in the various states they sample along their folding reaction coordinate, which can be accessed through the measurement of diffusion coefficients. It has been pointed out that the analysis of FCS curves is prone to artifacts that may lead to erroneous size determination. To set the stage for FCS studies of unfolded proteins, we first show that the diffusion coefficients of small molecules as well as proteins can be determined accurately even in the presence of high concentrations of co-solutes that change the solution refractive index significantly. Indeed, it is found that the Stokes-Einstein relation between the measured diffusion coefficient and solution viscosity holds even in highly concentrated glycerol or guanidinium hydrochloride (GuHCl) solutions. These measurements form the basis for an investigation of the structure of the denatured state of two proteins, the small protein L and the larger, three-domain protein adenylate kinase (AK). FCS is found useful for probing expansion in the denatured state beyond the unfolding transition. It is shown that the denatured state of protein L expands as the denaturant concentration increases, in a process akin to the transition from a globule to a coil in polymers. This process continues at least up to 5 M GuHCl. On the other hand, the denatured state of AK does not seem to expand much beyond 2 M GuHCl, a result that is in qualitative accord with single-molecule fluorescence histograms. Because both the unfolding transition and the coil-globule transition of AK occur at a much lower denaturant concentration than those of protein L, a possible correlation between the two phenomena is suggested.

MEASUREMENT OF 21 cm BRIGHTNESS FLUCTUATIONS AT z {approx} 0.8 IN CROSS-CORRELATION

Energy Technology Data Exchange (ETDEWEB)

Masui, K. W.; Switzer, E. R.; Calin, L.-M.; Pen, U.-L.; Shaw, J. R. [Canadian Institute for Theoretical Astrophysics, University of Toronto, 60 St. George St., Toronto, Ontario, M5S 3H8 (Canada); Banavar, N. [Department of Astronomy and Astrophysics, University of Toronto, 50 St. George St., Toronto, Ontario, M5S 3H4 (Canada); Bandura, K. [Department of Physics, McGill University, 3600 Rue University, Montreal, Quebec, H3A 2T8 (Canada); Blake, C. [Centre for Astrophysics and Supercomputing, Swinburne University of Technology, P.O. Box 218, Hawthorn, VIC 3122 (Australia); Chang, T.-C.; Liao, Y.-W. [Academia Sinica Institute of Astronomy and Astrophysics, P.O. Box 23-141, Taipei, 10617, Taiwan (China); Chen, X.; Li, Y.-C. [National Astronomical Observatories, Chinese Academy of Science, 20A Datun Road, Beijing 100012 (China); Natarajan, A.; Peterson, J. B.; Voytek, T. C. [McWilliams Center for Cosmology, Carnegie Mellon University, Department of Physics, 5000 Forbes Ave., Pittsburgh, PA 15213 (United States)

2013-01-20

In this Letter, 21 cm intensity maps acquired at the Green Bank Telescope are cross-correlated with large-scale structure traced by galaxies in the WiggleZ Dark Energy Survey. The data span the redshift range 0.6 < z < 1 over two fields totaling {approx}41 deg. sq. and 190 hr of radio integration time. The cross-correlation constrains {Omega}{sub HI} b{sub HI} r = [0.43 {+-} 0.07(stat.) {+-} 0.04(sys.)] Multiplication-Sign 10{sup -3}, where {Omega}{sub HI} is the neutral hydrogen (H I) fraction, r is the galaxy-hydrogen correlation coefficient, and b{sub HI} is the H I bias parameter. This is the most precise constraint on neutral hydrogen density fluctuations in a challenging redshift range. Our measurement improves the previous 21 cm cross-correlation at z {approx} 0.8 both in its precision and in the range of scales probed.

Fluorophore appended saccharide cyclophane: self-association, fluorescent properties, heterodimers with cyclodextrins, and cross-linking behavior with peanut agglutinin of dansyl-modified saccharide cyclophane.

Science.gov (United States)

Hayashida, Osamu; Hamachi, Itaru

2004-05-14

A saccharide cyclophane bearing an environment-sensitive fluorophore (1) was prepared by introducing not only three branches with a terminal galactose residue but also one with a dansyl moiety into a tetraaza[6.1.6.1]paracyclophane skeleton. Self-association behavior of the dansyl-appended saccharide cyclophane was characterized in aqueous media by fluorescence spectroscopy and dynamic light scattering measurements. At least in the concentrations below 1.0 x 10(-5) M, saccharide cyclophane 1 existed in a monomeric state, whereas it tended to form self-aggregated complexes in the higher concentration. Solvent polarity dependency on the emission spectra of 1 was examined by fluorescence spectroscopy. With increasing dioxane contents in dioxane/water solvents, the fluorescence intensity originating from the dansyl moiety of 1 increased along with a concomitant blue shift of the fluorescence maximum (lambda(em)). In the monomeric state of 1 in water, the dansyl moiety of 1 was not fully included into its cyclophane cavity but partially exposed to the bulk aqueous phase. In the higher concentration ranges in an aggregate state, however, the dansyl group of 1 was located in the apolar cyclophane cavity whose microenvironment was equivalent to the polarity of 1-butanol evaluated on the basis of a correlation between lambda(em) and solvent polarity. This indicates an intermolecular inclusion of the dansyl moiety within the cyclophane. When cyclodextrin (CD) was mixed with 1, the dansyl group of 1 was bound to an internal cavity of CD such as gamma-CD, beta-CD, 6-O-alpha-glucosyl-beta-CD, and 6-O-alpha-maltosyl-beta-CD with binding constants of 7.5 x 10(2), 7.8 x 10(2), 7.7 x 10(2), and 6.0 x 10(2) M(-1), respectively. Such a supramolecular assembling of dansyl-modified cyclophane 1 and CDs caused changes of the fluorescence spectra as well as appearance of induced CD bands in aqueous media. Furthermore, saccharide cyclophane 1 was selectively bound to peanut agglutinin

Temporal cross-correlation asymmetry and departure from equilibrium in a bistable chemical system.

Science.gov (United States)

Bianca, C; Lemarchand, A

2014-06-14

This paper aims at determining sustained reaction fluxes in a nonlinear chemical system driven in a nonequilibrium steady state. The method relies on the computation of cross-correlation functions for the internal fluctuations of chemical species concentrations. By employing Langevin-type equations, we derive approximate analytical formulas for the cross-correlation functions associated with nonlinear dynamics. Kinetic Monte Carlo simulations of the chemical master equation are performed in order to check the validity of the Langevin equations for a bistable chemical system. The two approaches are found in excellent agreement, except for critical parameter values where the bifurcation between monostability and bistability occurs. From the theoretical point of view, the results imply that the behavior of cross-correlation functions cannot be exploited to measure sustained reaction fluxes in a specific nonlinear system without the prior knowledge of the associated chemical mechanism and the rate constants.

Finite sample properties of power-law cross-correlations estimators

Czech Academy of Sciences Publication Activity Database

Krištoufek, Ladislav

2015-01-01

Roč. 419, č. 1 (2015), s. 513-525 ISSN 0378-4371 R&D Projects: GA ČR(CZ) GP14-11402P Keywords : power-law cross-correlations * long - term memory * econophysics Subject RIV: AH - Economics Impact factor: 1.785, year: 2015 http://library.utia.cas.cz/separaty/2014/E/kristoufek-0433530.pdf

Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy

Czech Academy of Sciences Publication Activity Database

Laňková, Martina; Humpolíčková, Jana; Vosolsobě, S.; Cit, Zdeněk; Lacek, Jozef; Čovan, Martin; Čovanová, Milada; Hof, Martin; Petrášek, Jan

2016-01-01

Roč. 22, č. 2 (2016), s. 290-299 ISSN 1431-9276 R&D Projects: GA ČR(CZ) GAP305/11/2476; GA ČR(CZ) GPP501/12/P951 Institutional support: RVO:61389030 ; RVO:61388955 Keywords : raster image correlation spectroscopy * fluorescence recovery after photobleaching * auxin influx Subject RIV: EB - Genetics ; Molecular Biology; CF - Physical ; Theoretical Chemistry (UFCH-W) Impact factor: 1.891, year: 2016

IMPROVING CORRELATION FUNCTION FITTING WITH RIDGE REGRESSION: APPLICATION TO CROSS-CORRELATION RECONSTRUCTION

International Nuclear Information System (INIS)

Matthews, Daniel J.; Newman, Jeffrey A.

2012-01-01

Cross-correlation techniques provide a promising avenue for calibrating photometric redshifts and determining redshift distributions using spectroscopy which is systematically incomplete (e.g., current deep spectroscopic surveys fail to obtain secure redshifts for 30%-50% or more of the galaxies targeted). In this paper, we improve on the redshift distribution reconstruction methods from our previous work by incorporating full covariance information into our correlation function fits. Correlation function measurements are strongly covariant between angular or spatial bins, and accounting for this in fitting can yield substantial reduction in errors. However, frequently the covariance matrices used in these calculations are determined from a relatively small set (dozens rather than hundreds) of subsamples or mock catalogs, resulting in noisy covariance matrices whose inversion is ill-conditioned and numerically unstable. We present here a method of conditioning the covariance matrix known as ridge regression which results in a more well behaved inversion than other techniques common in large-scale structure studies. We demonstrate that ridge regression significantly improves the determination of correlation function parameters. We then apply these improved techniques to the problem of reconstructing redshift distributions. By incorporating full covariance information, applying ridge regression, and changing the weighting of fields in obtaining average correlation functions, we obtain reductions in the mean redshift distribution reconstruction error of as much as ∼40% compared to previous methods. We provide a description of POWERFIT, an IDL code for performing power-law fits to correlation functions with ridge regression conditioning that we are making publicly available.

Groundwater travel time uncertainty analysis. Sensitivity of results to model geometry, and correlations and cross correlations among input parameters

International Nuclear Information System (INIS)

Clifton, P.M.

1985-03-01

This study examines the sensitivity of the travel time distribution predicted by a reference case model to (1) scale of representation of the model parameters, (2) size of the model domain, (3) correlation range of log-transmissivity, and (4) cross correlations between transmissivity and effective thickness. The basis for the reference model is the preliminary stochastic travel time model previously documented by the Basalt Waste Isolation Project. Results of this study show the following. The variability of the predicted travel times can be adequately represented when the ratio between the size of the zones used to represent the model parameters and the log-transmissivity correlation range is less than about one-fifth. The size of the model domain and the types of boundary conditions can have a strong impact on the distribution of travel times. Longer log-transmissivity correlation ranges cause larger variability in the predicted travel times. Positive cross correlation between transmissivity and effective thickness causes a decrease in the travel time variability. These results demonstrate the need for a sound conceptual model prior to conducting a stochastic travel time analysis

Fluorescence Correlation Spectroscopy to find the critical balance between extracellular association and intracellular dissociation of mRNA-complexes.

Science.gov (United States)

Zhang, Heyang; De Smedt, Stefaan C; Remaut, Katrien

2018-05-10

Fluorescence Correlation Spectroscopy (FCS) is a promising tool to study interactions on a single molecule level. The diffusion of fluorescent molecules in and out of the excitation volume of a confocal microscope leads to the fluorescence fluctuations that give information on the average number of fluorescent molecules present in the excitation volume and their diffusion coefficients. In this context, we complexed mRNA into lipoplexes and polyplexes and explored the association/dissociation degree of complexes by using gel electrophoresis and FCS. FCS enabled us to measure the association and dissociation degree of mRNA-based complexes both in buffer and protein-rich biological fluids such as human serum and ascitic fluid, which is a clear advantage over gel electrophoresis that was only applicable in protein-free buffer solutions. Furthermore, following the complex stability in buffer and biological fluids by FCS assisted to understand how complex characteristics, such as charge ratio and strength of mRNA binding, correlated to the transfection efficiency. We found that linear polyethyleneimine prevented efficient translation of mRNA, most likely due to a too strong mRNA binding, whereas the lipid based carrier Lipofectamine ® messengerMAX did succeed in efficient release and subsequent translation of mRNA in the cytoplasm of the cells. Overall, FCS is a reliable tool for the in depth characterization of mRNA complexes and can help us to find the critical balance keeping mRNA bound in complexes in the extracellular environment and efficient intracellular mRNA release leading to protein production. The delivery of messenger RNA (mRNA) to cells is promising to treat a variety of diseases. Therefore, the mRNA is typically packed in small lipid particles or polymer particles that help the mRNA to reach the cytoplasm of the cells. These particles should bind and carry the mRNA in the extracellular environment (e.g. blood, peritoneal fluid, ...), but should release

Cross-correlation analysis between Chinese TF contracts and treasury ETF based on high-frequency data

Science.gov (United States)

Zhou, Yu; Chen, Shi

2016-02-01

In this paper, we investigate the high-frequency cross-correlation relationship between Chinese treasury futures contracts and treasury ETF. We analyze the logarithmic return of these two price series, from which we can conclude that both return series are not normally distributed and the futures markets have greater volatility. We find significant cross-correlation between these two series. We further confirm the relationship using the DCCA coefficient and the DMCA coefficient. We quantify the long-range cross-correlation with DCCA method, and we further show that the relationship is multifractal. An arbitrage algorithm based on DFA regression with stable return is proposed in the last part.

Mitigating fluorescence spectral overlap in wide-field endoscopic imaging

Science.gov (United States)

Hou, Vivian; Nelson, Leonard Y.; Seibel, Eric J.

2013-01-01

Abstract. The number of molecular species suitable for multispectral fluorescence imaging is limited due to the overlap of the emission spectra of indicator fluorophores, e.g., dyes and nanoparticles. To remove fluorophore emission cross-talk in wide-field multispectral fluorescence molecular imaging, we evaluate three different solutions: (1) image stitching, (2) concurrent imaging with cross-talk ratio subtraction algorithm, and (3) frame-sequential imaging. A phantom with fluorophore emission cross-talk is fabricated, and a 1.2-mm ultrathin scanning fiber endoscope (SFE) is used to test and compare these approaches. Results show that fluorophore emission cross-talk could be successfully avoided or significantly reduced. Near term, the concurrent imaging method of wide-field multispectral fluorescence SFE is viable for early stage cancer detection and localization in vivo. Furthermore, a means to enhance exogenous fluorescence target-to-background ratio by the reduction of tissue autofluorescence background is demonstrated. PMID:23966226

Confined diffusion in tubular structures analyzed by fluorescence correlation spectroscopy on a mirror

International Nuclear Information System (INIS)

Etienne, Emilien; Lenne, Pierre-Francois; Sturgis, James N.; Rigneault, Herve

2006-01-01

In fluorescence correlation spectroscopy (FCS) analysis it is generally assumed that molecular species diffuse freely in volumes much larger than the three-dimensional FCS observation volume. However, this standard assumption is not valid in many measurement conditions, particularly in tubular structures with diameters in the micrometer range, such as those found in living cells (organelles, dendrites) and microfluidic devices (capillaries,reaction chambers). As a result the measured autocorrelation functions (ACFs) deviate from those predicted for free diffusion, and this can shift the measured diffusion coefficient by as much as ∼50% when the tube diameter is comparable with the axial extension of the FCS observation volume. We show that the range of validity of the FCS measurements can be drastically improved if the tubular structures are located in the close vicinity of a mirror on which FCS is performed. In this case a new fluctuation time in the ACF, arising from the diffusion of fluorescent probes in optical fringes,permits measurement of the real diffusion coefficient within the tubular structure without assumptions about either the confined geometry orthe FCS observation volume geometry. We show that such a measurement can be done when the tubular structure contains at least one pair of dark and bright fringes resulting from interference between the incoming and the reflected excitation beams on the mirror surface. Measurement of the diffusion coefficient of the enhanced green fluorescent protein (EGFP) and IscS-EGFP in the cytoplasm of living Escherichiacoli illustrates the capabilities of the technique

Characteristics of angular cross correlations studied by light scattering from two-dimensional microsphere films

Science.gov (United States)

Schroer, M. A.; Gutt, C.; Grübel, G.

2014-07-01

Recently the analysis of scattering patterns by angular cross-correlation analysis (CCA) was introduced to reveal the orientational order in disordered samples with special focus to future applications on x-ray free-electron laser facilities. We apply this CCA approach to ultra-small-angle light-scattering data obtained from two-dimensional monolayers of microspheres. The films were studied in addition by optical microscopy. This combined approach allows to calculate the cross-correlations of the scattering patterns, characterized by the orientational correlation function Ψl(q), as well as to obtain the real-space structure of the monolayers. We show that CCA is sensitive to the orientational order of monolayers formed by the microspheres which are not directly visible from the scattering patterns. By mixing microspheres of different radii the sizes of ordered monolayer domains is reduced. For these samples it is shown that Ψl(q) quantitatively describes the degree of hexagonal order of the two-dimensional films. The experimental CCA results are compared with calculations based on the microscopy images. Both techniques show qualitatively similar features. Differences can be attributed to the wave-front distortion of the laser beam in the experiment. This effect is discussed by investigating the effect of different wave fronts on the cross-correlation analysis results. The so-determined characteristics of the cross-correlation analysis will be also relevant for future x-ray-based studies.

Cross-correlation of instantaneous phase increments in pressure-flow fluctuations: Applications to cerebral autoregulation

Science.gov (United States)

Chen, Zhi; Hu, Kun; Stanley, H. Eugene; Novak, Vera; Ivanov, Plamen Ch.

2006-03-01

We investigate the relationship between the blood flow velocities (BFV) in the middle cerebral arteries and beat-to-beat blood pressure (BP) recorded from a finger in healthy and post-stroke subjects during the quasisteady state after perturbation for four different physiologic conditions: supine rest, head-up tilt, hyperventilation, and CO2 rebreathing in upright position. To evaluate whether instantaneous BP changes in the steady state are coupled with instantaneous changes in the BFV, we compare dynamical patterns in the instantaneous phases of these signals, obtained from the Hilbert transform, as a function of time. We find that in post-stroke subjects the instantaneous phase increments of BP and BFV exhibit well-pronounced patterns that remain stable in time for all four physiologic conditions, while in healthy subjects these patterns are different, less pronounced, and more variable. We propose an approach based on the cross-correlation of the instantaneous phase increments to quantify the coupling between BP and BFV signals. We find that the maximum correlation strength is different for the two groups and for the different conditions. For healthy subjects the amplitude of the cross-correlation between the instantaneous phase increments of BP and BFV is small and attenuates within 3-5 heartbeats. In contrast, for post-stroke subjects, this amplitude is significantly larger and cross-correlations persist up to 20 heartbeats. Further, we show that the instantaneous phase increments of BP and BFV are cross-correlated even within a single heartbeat cycle. We compare the results of our approach with three complementary methods: direct BP-BFV cross-correlation, transfer function analysis, and phase synchronization analysis. Our findings provide insight into the mechanism of cerebral vascular control in healthy subjects, suggesting that this control mechanism may involve rapid adjustments (within a heartbeat) of the cerebral vessels, so that BFV remains steady in

Quantification of Material Fluorescence and Light Scattering Cross Sections Using Ratiometric Bandwidth-Varied Polarized Resonance Synchronous Spectroscopy.

Science.gov (United States)

Xu, Joanna Xiuzhu; Hu, Juan; Zhang, Dongmao

2018-05-25

Presented herein is the ratiometric bandwidth-varied polarized resonance synchronous spectroscopy (BVPRS2) method for quantification of material optical activity spectra. These include the sample light absorption and scattering cross-section spectrum, the scattering depolarization spectrum, and the fluorescence emission cross-section and depolarization spectrum in the wavelength region where the sample both absorbs and emits. This ratiometric BVPRS2 spectroscopic method is a self-contained technique capable of quantitatively decoupling material fluorescence and light scattering signal contribution to its ratiometric BVPRS2 spectra through the linear curve-fitting of the ratiometric BVPRS2 signal as a function of the wavelength bandwidth used in the PRS2 measurements. Example applications of this new spectroscopic method are demonstrated with materials that can be approximated as pure scatterers, simultaneous photon absorbers/emitters, simultaneous photon absorbers/scatterers, and finally simultaneous photon absorbers/scatterers/emitters. Because the only instruments needed for this ratiometric BVPRS2 technique are the conventional UV-vis spectrophotometer and spectrofluorometer, this work should open doors for routine decomposition of material UV-vis extinction spectrum into its absorption and scattering component spectra. The methodology and insights provided in this work should be of broad significance to all chemical research that involves photon/matter interactions.

The large-scale quasar-Lyman α forest cross-correlation from BOSS

Energy Technology Data Exchange (ETDEWEB)

Font-Ribera, Andreu [Institute of Theoretical Physics, University of Zurich, Winterthurerstrasse 190, 8057 Zurich (Switzerland); Arnau, Eduard [Institut de Ciències del Cosmos (IEEC/UB), Martí i Franquès 1, 08028 Barcelona, Catalonia (Spain); Miralda-Escudé, Jordi, E-mail: font@physik.uzh.ch, E-mail: edu.arnau.lazaro@gmail.com, E-mail: miralda@icc.ub.edu [Institució Catalana de Recerca i Estudis Avançats, Passeig Lluís Companys 23, 08010 Barcelona, Catalonia (Spain); and others

2013-05-01

We measure the large-scale cross-correlation of quasars with the Lyα forest absorption in redshift space, using ∼ 60000 quasar spectra from Data Release 9 (DR9) of the Baryon Oscillation Spectroscopic Survey (BOSS). The cross-correlation is detected over a wide range of scales, up to comoving separations r of 80 h{sup −1}Mpc. For r > 15 h{sup −1}Mpc, we show that the cross-correlation is well fitted by the linear theory prediction for the mean overdensity around a quasar host halo in the standard ΛCDM model, with the redshift distortions indicative of gravitational evolution detected at high confidence. Using previous determinations of the Lyα forest bias factor obtained from the Lyα autocorrelation, we infer the quasar bias factor to be b{sub q} = 3.64{sup +0.13}{sub −0.15} at a mean redshift z = 2.38, in agreement with previous measurements from the quasar auto-correlation. We also obtain a new estimate of the Lyα forest redshift distortion factor, β{sub F} = 1.1±0.15, slightly larger than but consistent with the previous measurement from the Lyα forest autocorrelation. The simple linear model we use fails at separations r < 15h{sup −1}Mpc, and we show that this may reasonably be due to the enhanced ionization due to radiation from the quasars. We also provide the expected correction that the mass overdensity around the quasar implies for measurements of the ionizing radiation background from the line-of-sight proximity effect.

The large-scale quasar-Lyman α forest cross-correlation from BOSS

International Nuclear Information System (INIS)

Font-Ribera, Andreu; Arnau, Eduard; Miralda-Escudé, Jordi

2013-01-01

We measure the large-scale cross-correlation of quasars with the Lyα forest absorption in redshift space, using ∼ 60000 quasar spectra from Data Release 9 (DR9) of the Baryon Oscillation Spectroscopic Survey (BOSS). The cross-correlation is detected over a wide range of scales, up to comoving separations r of 80 h −1 Mpc. For r > 15 h −1 Mpc, we show that the cross-correlation is well fitted by the linear theory prediction for the mean overdensity around a quasar host halo in the standard ΛCDM model, with the redshift distortions indicative of gravitational evolution detected at high confidence. Using previous determinations of the Lyα forest bias factor obtained from the Lyα autocorrelation, we infer the quasar bias factor to be b q = 3.64 +0.13 −0.15 at a mean redshift z = 2.38, in agreement with previous measurements from the quasar auto-correlation. We also obtain a new estimate of the Lyα forest redshift distortion factor, β F = 1.1±0.15, slightly larger than but consistent with the previous measurement from the Lyα forest autocorrelation. The simple linear model we use fails at separations r −1 Mpc, and we show that this may reasonably be due to the enhanced ionization due to radiation from the quasars. We also provide the expected correction that the mass overdensity around the quasar implies for measurements of the ionizing radiation background from the line-of-sight proximity effect

Dynamical Analysis of Stock Market Instability by Cross-correlation Matrix

Science.gov (United States)

Takaishi, Tetsuya

2016-08-01

We study stock market instability by using cross-correlations constructed from the return time series of 366 stocks traded on the Tokyo Stock Exchange from January 5, 1998 to December 30, 2013. To investigate the dynamical evolution of the cross-correlations, crosscorrelation matrices are calculated with a rolling window of 400 days. To quantify the volatile market stages where the potential risk is high, we apply the principal components analysis and measure the cumulative risk fraction (CRF), which is the system variance associated with the first few principal components. From the CRF, we detected three volatile market stages corresponding to the bankruptcy of Lehman Brothers, the 2011 Tohoku Region Pacific Coast Earthquake, and the FRB QE3 reduction observation in the study period. We further apply the random matrix theory for the risk analysis and find that the first eigenvector is more equally de-localized when the market is volatile.

Dynamical Analysis of Stock Market Instability by Cross-correlation Matrix

International Nuclear Information System (INIS)

Takaishi, Tetsuya

2016-01-01

We study stock market instability by using cross-correlations constructed from the return time series of 366 stocks traded on the Tokyo Stock Exchange from January 5, 1998 to December 30, 2013. To investigate the dynamical evolution of the cross-correlations, crosscorrelation matrices are calculated with a rolling window of 400 days. To quantify the volatile market stages where the potential risk is high, we apply the principal components analysis and measure the cumulative risk fraction (CRF), which is the system variance associated with the first few principal components. From the CRF, we detected three volatile market stages corresponding to the bankruptcy of Lehman Brothers, the 2011 Tohoku Region Pacific Coast Earthquake, and the FRB QE3 reduction observation in the study period. We further apply the random matrix theory for the risk analysis and find that the first eigenvector is more equally de-localized when the market is volatile. (paper)

Applying fluorescence correlation spectroscopy to investigate peptide-induced membrane disruption

DEFF Research Database (Denmark)

Kristensen, Kasper; Henriksen, Jonas Rosager; Andresen, Thomas Lars

2017-01-01

to quantify leakage of fluorescent molecules of different sizes from large unilamellar lipid vesicles, thereby providing a tool for estimating the size of peptide-induced membrane disruptions. If fluorescently labeled lipids are incorporated into the membranes of the vesicles, FCS can also be used to obtain...

Financial liberalization and stock market cross-correlation: MF-DCCA analysis based on Shanghai-Hong Kong Stock Connect

Science.gov (United States)

Ruan, Qingsong; Zhang, Shuhua; Lv, Dayong; Lu, Xinsheng

2018-02-01

Based on the implementation of Shanghai-Hong Kong Stock Connect in China, this paper examines the effects of financial liberalization on stock market comovement using both multifractal detrended fluctuation analysis (MF-DFA) and multifractal detrended cross-correlation analysis (MF-DCCA) methods. Results based on MF-DFA confirm the multifractality of Shanghai and Hong Kong stock markets, and the market efficiency of Shanghai stock market increased after the implementation of this connect program. Besides, analysis based on MF-DCCA has verified the existence of persistent cross-correlation between Shanghai and Hong Kong stock markets, and the cross-correlation gets stronger after the launch of this liberalization program. Finally, we find that fat-tail distribution is the main source of multifractality in the cross-correlations before the stock connect program, while long-range correlation contributes to the multifractality after this program.

Multifractal cross-correlations between crude oil and tanker freight rate

Science.gov (United States)

Chen, Feier; Miao, Yuqi; Tian, Kang; Ding, Xiaoxu; Li, Tingyi

2017-05-01

Analysis of crude oil price and tanker freight rate volatility attract more attention as the mechanism is not only the basis of industrialization but also a vital role in economics, especially after the year 2008 when financial crisis notably blew the maritime transportation. In this paper, we studied the cross-correlations between the West Texas International crude oil (WTI) and Baltic Exchange Dirty Tanker Index (BDTI) employing the Multifractal Detrended Cross-Correlation Analysis (MF-DCCA). Empirical results show that the degree of short-term cross-correlation is higher than that in the long term and that the strength of multifractality after financial crisis is larger than that before. Moreover, the components of multifractal spectrum are quantified with the finite-size effect taken into consideration and an improved method in terms of constructing the surrogated time series provided. Numerical results show that the multifractality is generated mostly from the nonlinear and the fat-tailed probability distribution (PDF) part. Also, it is apparent that the PDF part changes a lot after the financial crisis. The research is contributory to risk management by providing various instructions for participants in shipping markets. Our main contribution is that we investigated both the multifractal features and the origin of multifractality and provided confirming evidence of multifractality through numerical results while applying quantitative analysis based on MF-DCCA; furthermore, the research is contributory to risk management since it provides instructions in both economic market and stock market simultaneously. However, constructing the surrogated series in order to obtain consistence seems less convincing which requires further discussion and attempts.

The endogenous fluorescence of fibroblast in collagen gels as indicator of stiffness of the extracellular matrix

Science.gov (United States)

Padilla-Martinez, J. P.; Ortega-Martinez, A.; Franco, W.

2016-03-01

The stiffness or rigidity of the extracellular matrix (ECM) regulates cell response. Established mechanical tests to measure stiffness, such as indentation and tensile tests, are invasive and destructive to the sample. Endogenous or native molecules to cells and ECM components, like tryptophan and cross-links of collagen, display fluorescence upon irradiation with ultraviolet light. Most likely, the concentration of these endogenous fluorophores changes as the stiffness of the ECM changes. In this work we investigate the endogenous fluorescence of collagen gels containing fibroblasts as a non-invasive non-destructive method to measure stiffness of the ECM. Human fibroblast cells were cultured in three-dimensional gels of type I collagen (50,000 cells/ml). This construct is a simple model of tissue contraction. During contraction, changes in the excitation-emission matrix (a fluorescence map in the 240-520/290-530 nm range) of constructs were measured with a spectrofluoremeter, and changes in stiffness were measured with a standard indentation test over 16 days. Results show that a progressive increase in fluorescence of the 290/340 nm excitation-emission pair correlates with a progressive increase in stiffness (r=0.9, α=0.5). The fluorescence of this excitation-emission pair is ascribed to tryptophan and variations in the fluorescence of this pair correlate with cellular proliferation. In this tissue model, the endogenous functional fluorescence of proliferating fibroblast cells is a biomechanical marker of stiffness of the ECM.

Atmospheric pollution measurement by optical cross correlation methods - A concept

Science.gov (United States)

Fisher, M. J.; Krause, F. R.

1971-01-01

Method combines standard spectroscopy with statistical cross correlation analysis of two narrow light beams for remote sensing to detect foreign matter of given particulate size and consistency. Method is applicable in studies of generation and motion of clouds, nuclear debris, ozone, and radiation belts.

Confined detection volume of fluorescence correlation spectroscopy by bare fiber probes.

Science.gov (United States)

Lu, Guowei; Lei, Franck H; Angiboust, Jean-François; Manfait, Michel

2010-04-01

A fiber-tip-based near-field fluorescence correlation spectroscopy (FCS) has been developed for confining the detection volume to sub-diffraction-limited dimensions. This near-field FCS is based on near-field illumination by coupling a scanning near-field optical microscope (SNOM) to a conventional confocal FCS. Single-molecule FCS analysis at 100 nM Rhodamine 6G has been achieved by using bare chemically etched, tapered fiber tips. The detection volume under control of the SNOM system has been reduced over one order of magnitude compared to that of the conventional confocal FCS. Related factors influencing the near-field FCS performance are investigated and discussed in detail. In this proof-of-principle study, the preliminary experimental results suggest that the fiber-tip-based near-field FCS might be a good alternative to realize localized analysis at the single-molecule level.

Importance of Preserving Cross-correlation in developing Statistically Downscaled Climate Forcings and in estimating Land-surface Fluxes and States

Science.gov (United States)

Das Bhowmik, R.; Arumugam, S.

2015-12-01

Multivariate downscaling techniques exhibited superiority over univariate regression schemes in terms of preserving cross-correlations between multiple variables- precipitation and temperature - from GCMs. This study focuses on two aspects: (a) develop an analytical solutions on estimating biases in cross-correlations from univariate downscaling approaches and (b) quantify the uncertainty in land-surface states and fluxes due to biases in cross-correlations in downscaled climate forcings. Both these aspects are evaluated using climate forcings available from both historical climate simulations and CMIP5 hindcasts over the entire US. The analytical solution basically relates the univariate regression parameters, co-efficient of determination of regression and the co-variance ratio between GCM and downscaled values. The analytical solutions are compared with the downscaled univariate forcings by choosing the desired p-value (Type-1 error) in preserving the observed cross-correlation. . For quantifying the impacts of biases on cross-correlation on estimating streamflow and groundwater, we corrupt the downscaled climate forcings with different cross-correlation structure.

Direct Vpr-Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging

Directory of Open Access Journals (Sweden)

Mély Yves

2008-09-01

Full Text Available Abstract Background The human immunodeficiency virus type 1 (HIV-1 encodes several regulatory proteins, notably Vpr which influences the survival of the infected cells by causing a G2/M arrest and apoptosis. Such an important role of Vpr in HIV-1 disease progression has fuelled a large number of studies, from its 3D structure to the characterization of specific cellular partners. However, no direct imaging and quantification of Vpr-Vpr interaction in living cells has yet been reported. To address this issue, eGFP- and mCherry proteins were tagged by Vpr, expressed in HeLa cells and their interaction was studied by two photon fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy. Results Results show that Vpr forms homo-oligomers at or close to the nuclear envelope. Moreover, Vpr dimers and trimers were found in the cytoplasm and in the nucleus. Point mutations in the three α helices of Vpr drastically impaired Vpr oligomerization and localization at the nuclear envelope while point mutations outside the helical regions had no effect. Theoretical structures of Vpr mutants reveal that mutations within the α-helices could perturb the leucine zipper like motifs. The ΔQ44 mutation has the most drastic effect since it likely disrupts the second helix. Finally, all Vpr point mutants caused cell apoptosis suggesting that Vpr-mediated apoptosis functions independently from Vpr oligomerization. Conclusion We report that Vpr oligomerization in HeLa cells relies on the hydrophobic core formed by the three α helices. This oligomerization is required for Vpr localization at the nuclear envelope but not for Vpr-mediated apoptosis.

Multifractal detrended cross-correlation analysis for epileptic patient in seizure and seizure free status

International Nuclear Information System (INIS)

Ghosh, Dipak; Dutta, Srimonti; Chakraborty, Sayantan

2014-01-01

Highlights: • We analyze EEG of patients during seizure and in seizure free interval. • Data from different sections of the brain and seizure activity was analyzed. • Assessment of cross-correlation in seizure and seizure free interval using MF-DXA technique. - Abstract: This paper reports a study of EEG data of epileptic patients in terms of multifractal detrended cross-correlation analysis (MF-DXA). The EEG clinical data were obtained from the EEG Database available with the Clinic of Epileptology of the University Hospital of Bonn, Germany. The data sets (C, D, and E) were taken from five epileptic patients undergoing presurgical evaluations. The data sets consist of intracranial EEG recordings during seizure-free intervals (interictal periods) from within the epileptogenic zone (D) and from the hippocampal formation of the opposite hemisphere of the epileptic patients’ brain, respectively (C). The data set (E) was recorded during seizure activity (ictal periods). MF-DXA is a very rigorous and robust tool for assessment of cross-correlation among two nonlinear time series. The study reveals the degree of cross-correlation is more among seizure and seizure free interval in epileptogenic zone. These data are very significant for diagnosis, onset and prognosis of epileptic patients

A new detrended semipartial cross-correlation analysis: Assessing the important meteorological factors affecting API

International Nuclear Information System (INIS)

Shen, Chen-Hua

2015-01-01

To analyze the unique contribution of meteorological factors to the air pollution index (API), a new method, the detrended semipartial cross-correlation analysis (DSPCCA), is proposed. Based on both a detrended cross-correlation analysis and a DFA-based multivariate-linear-regression (DMLR), this method is improved by including a semipartial correlation technique, which is used to indicate the unique contribution of an explanatory variable to multiple correlation coefficients. The advantages of this method in handling nonstationary time series are illustrated by numerical tests. To further demonstrate the utility of this method in environmental systems, new evidence of the primary contribution of meteorological factors to API is provided through DMLR. Results show that the most important meteorological factors affecting API are wind speed and diurnal temperature range, and the explanatory ability of meteorological factors to API gradually strengthens with increasing time scales. The results suggest that DSPCCA is a useful method for addressing environmental systems. - Highlights: • A detrended multiple linear regression is shown. • A detrended semipartial cross correlation analysis is proposed. • The important meteorological factors affecting API are assessed. • The explanatory ability of meteorological factors to API gradually strengthens with increasing time scales.

A new detrended semipartial cross-correlation analysis: Assessing the important meteorological factors affecting API

Energy Technology Data Exchange (ETDEWEB)

Shen, Chen-Hua, E-mail: shenandchen01@163.com [College of Geographical Science, Nanjing Normal University, Nanjing 210046 (China); Jiangsu Center for Collaborative Innovation in Geographical Information Resource, Nanjing 210046 (China); Key Laboratory of Virtual Geographic Environment of Ministry of Education, Nanjing 210046 (China)

2015-12-04

To analyze the unique contribution of meteorological factors to the air pollution index (API), a new method, the detrended semipartial cross-correlation analysis (DSPCCA), is proposed. Based on both a detrended cross-correlation analysis and a DFA-based multivariate-linear-regression (DMLR), this method is improved by including a semipartial correlation technique, which is used to indicate the unique contribution of an explanatory variable to multiple correlation coefficients. The advantages of this method in handling nonstationary time series are illustrated by numerical tests. To further demonstrate the utility of this method in environmental systems, new evidence of the primary contribution of meteorological factors to API is provided through DMLR. Results show that the most important meteorological factors affecting API are wind speed and diurnal temperature range, and the explanatory ability of meteorological factors to API gradually strengthens with increasing time scales. The results suggest that DSPCCA is a useful method for addressing environmental systems. - Highlights: • A detrended multiple linear regression is shown. • A detrended semipartial cross correlation analysis is proposed. • The important meteorological factors affecting API are assessed. • The explanatory ability of meteorological factors to API gradually strengthens with increasing time scales.

Extended families of 2D arrays with near optimal auto and low cross-correlation

Science.gov (United States)

Svalbe, I. D.; Tirkel, A. Z.

2017-12-01

Families of 2D arrays can be constructed where each array has perfect autocorrelation, and the cross-correlation between any pair of family members is optimally low. We exploit equivalent Hadamard matrices to construct many families of p p × p arrays, where p is any 4k-1 prime. From these families, we assemble extended families of arrays with members that exhibit perfect autocorrelation and next-to-optimally low cross-correlation. Pseudo-Hadamard matrices are used to construct extended families using p = 4k + 1 primes. An optimal family of 31 31 × 31 perfect arrays can provide copyright protection to uniquely stamp a robust, low-visibility watermark within every frame of each second of high-definition, 30 fps video. The extended families permit the embedding of many more perfect watermarks that have next-to-minimal cross-correlations.

A flexible fluorescence correlation spectroscopy based method for quantification of the DNA double labeling efficiency with precision control

International Nuclear Information System (INIS)

Hou, Sen; Tabaka, Marcin; Sun, Lili; Trochimczyk, Piotr; Kaminski, Tomasz S; Kalwarczyk, Tomasz; Zhang, Xuzhu; Holyst, Robert

2014-01-01

We developed a laser-based method to quantify the double labeling efficiency of double-stranded DNA (dsDNA) in a fluorescent dsDNA pool with fluorescence correlation spectroscopy (FCS). Though, for quantitative biochemistry, accurate measurement of this parameter is of critical importance, before our work it was almost impossible to quantify what percentage of DNA is doubly labeled with the same dye. The dsDNA is produced by annealing complementary single-stranded DNA (ssDNA) labeled with the same dye at 5′ end. Due to imperfect ssDNA labeling, the resulting dsDNA is a mixture of doubly labeled dsDNA, singly labeled dsDNA and unlabeled dsDNA. Our method allows the percentage of doubly labeled dsDNA in the total fluorescent dsDNA pool to be measured. In this method, we excite the imperfectly labeled dsDNA sample in a focal volume of <1 fL with a laser beam and correlate the fluctuations of the fluorescence signal to get the FCS autocorrelation curves; we express the amplitudes of the autocorrelation function as a function of the DNA labeling efficiency; we perform a comparative analysis of a dsDNA sample and a reference dsDNA sample, which is prepared by increasing the total dsDNA concentration c (c > 1) times by adding unlabeled ssDNA during the annealing process. The method is flexible in that it allows for the selection of the reference sample and the c value can be adjusted as needed for a specific study. We express the precision of the method as a function of the ssDNA labeling efficiency or the dsDNA double labeling efficiency. The measurement precision can be controlled by changing the c value. (letter)

Lenses in the forest: cross correlation of the Lyman-alpha flux with cosmic microwave background lensing.

Science.gov (United States)

Vallinotto, Alberto; Das, Sudeep; Spergel, David N; Viel, Matteo

2009-08-28

We present a theoretical estimate for a new observable: the cross correlation between the Lyman-alpha flux fluctuations in quasar spectra and the convergence of the cosmic microwave background as measured along the same line of sight. As a first step toward the assessment of its detectability, we estimate the signal-to-noise ratio using linear theory. Although the signal-to-noise is small for a single line of sight and peaks at somewhat smaller redshifts than those probed by the Lyman-alpha forest, we estimate a total signal-to-noise of 9 for cross correlating quasar spectra of SDSS-III with Planck and 20 for cross correlating with a future polarization based cosmic microwave background experiment. The detection of this effect would be a direct measure of the neutral hydrogen-matter cross correlation and could provide important information on the growth of structures at large scales in a redshift range which is still poorly probed.

Determination of Equilibrium Constant and Relative Brightness in FRET-FCS by Including the Third-Order Correlations.

Science.gov (United States)

Meng, Lingyi; He, Shanshan; Zhao, Xin Sheng

2017-12-21

Fluorescence correlation spectroscopy (FCS) encodes the information on the equilibrium constant (K), the relative fluorescence brightness of fluorophore (Q), and the forward and backward reaction rate constants (k + and k - ) on a physical or chemical relaxation. However, it has been a long-standing problem to completely resolve the FCS data to get the thermodynamic and kinetic information. Recently, we have solved the problem for fluorescence autocorrelation spectroscopy (FACS). Here, we extend the method to fluorescence cross-correlation spectroscopy (FCCS), which appears when FCS is coupled with fluorescence resonance energy transfer (FRET). Among 12 total second-order and third-order pre-exponential factors in a relaxation process probed by the FRET-FCS technique, 3 are independent. We presented and discussed 3 sets of explicit solutions to use these pre-exponential factors to calculate K and Q. Together with the relaxation time, the acquired K will allow people to obtain k + and k - , so that the goal of deciphering the FRET-FCS data will be fully reached. The theory is verified by extensive computer simulations and tested experimentally on a system of oligonucleotide hybridization.

Measurement of the dipole in the cross-correlation function of galaxies

CERN Document Server

Gaztanaga, Enrique; Hui, Lam

2017-01-01

It is usually assumed that in the linear regime the two-point correlation function of galaxies contains only a monopole, quadrupole and hexadecapole. Looking at cross-correlations between different populations of galaxies, this turns out not to be the case. In particular, the cross-correlations between a bright and a faint population of galaxies contain also a dipole. In this paper we present the first attempt to measure this dipole. We discuss the four types of effects that contribute to the dipole: relativistic distortions, evolution effect, wide-angle effect and large-angle effect. We show that the first three contributions are intrinsic anti-symmetric contributions that do not depend on the choice of angle used to measure the dipole. On the other hand the large-angle effect appears only if the angle chosen to extract the dipole breaks the symmetry of the problem. We show that the relativistic distortions, the evolution effect and the wide-angle effect are too small to be detected in the LOWz and CMASS sam...

Development of windows based software to analyze fluorescence decay with time-correlated single photon counting (TCSPC) setup

International Nuclear Information System (INIS)

Mallick, M.B.; Ravindranath, S.V.G.; Das, N.C.

2002-07-01

A VUV spectroscopic facility for studies in photophysics and photochemistry is being set up at INDUS-I synchrotron source, CAT, Indore. For this purpose, a data acquisition system based on time-correlated single photon counting method is being developed for fluorescence lifetime measurement. To estimate fluorescence lifetime from the data collected with this sytem, a Windows based program has been developed using Visual Basic 5.0. It uses instrument response function (IRF) and observed decay curve and estimates parameters of single exponential decay by least square analysis and Marquardt method as convergence mechanism. Estimation of parameters was performed using data collected with a commercial setup. Goodness of fit was judged by evaluating χR 2 , weighted residuals and autocorrelation function. Performance is compared with two commercial software packages and found to be satisfactory. (author)

Quantifying and modeling long-range cross correlations in multiple time series with applications to world stock indices.

Science.gov (United States)

Wang, Duan; Podobnik, Boris; Horvatić, Davor; Stanley, H Eugene

2011-04-01

We propose a modified time lag random matrix theory in order to study time-lag cross correlations in multiple time series. We apply the method to 48 world indices, one for each of 48 different countries. We find long-range power-law cross correlations in the absolute values of returns that quantify risk, and find that they decay much more slowly than cross correlations between the returns. The magnitude of the cross correlations constitutes "bad news" for international investment managers who may believe that risk is reduced by diversifying across countries. We find that when a market shock is transmitted around the world, the risk decays very slowly. We explain these time-lag cross correlations by introducing a global factor model (GFM) in which all index returns fluctuate in response to a single global factor. For each pair of individual time series of returns, the cross correlations between returns (or magnitudes) can be modeled with the autocorrelations of the global factor returns (or magnitudes). We estimate the global factor using principal component analysis, which minimizes the variance of the residuals after removing the global trend. Using random matrix theory, a significant fraction of the world index cross correlations can be explained by the global factor, which supports the utility of the GFM. We demonstrate applications of the GFM in forecasting risks at the world level, and in finding uncorrelated individual indices. We find ten indices that are practically uncorrelated with the global factor and with the remainder of the world indices, which is relevant information for world managers in reducing their portfolio risk. Finally, we argue that this general method can be applied to a wide range of phenomena in which time series are measured, ranging from seismology and physiology to atmospheric geophysics.

Quantifying and modeling long-range cross correlations in multiple time series with applications to world stock indices

Science.gov (United States)

Wang, Duan; Podobnik, Boris; Horvatić, Davor; Stanley, H. Eugene

2011-04-01

We propose a modified time lag random matrix theory in order to study time-lag cross correlations in multiple time series. We apply the method to 48 world indices, one for each of 48 different countries. We find long-range power-law cross correlations in the absolute values of returns that quantify risk, and find that they decay much more slowly than cross correlations between the returns. The magnitude of the cross correlations constitutes “bad news” for international investment managers who may believe that risk is reduced by diversifying across countries. We find that when a market shock is transmitted around the world, the risk decays very slowly. We explain these time-lag cross correlations by introducing a global factor model (GFM) in which all index returns fluctuate in response to a single global factor. For each pair of individual time series of returns, the cross correlations between returns (or magnitudes) can be modeled with the autocorrelations of the global factor returns (or magnitudes). We estimate the global factor using principal component analysis, which minimizes the variance of the residuals after removing the global trend. Using random matrix theory, a significant fraction of the world index cross correlations can be explained by the global factor, which supports the utility of the GFM. We demonstrate applications of the GFM in forecasting risks at the world level, and in finding uncorrelated individual indices. We find ten indices that are practically uncorrelated with the global factor and with the remainder of the world indices, which is relevant information for world managers in reducing their portfolio risk. Finally, we argue that this general method can be applied to a wide range of phenomena in which time series are measured, ranging from seismology and physiology to atmospheric geophysics.

Kinetic description of the total photoabsorption cross section: correlation effects

International Nuclear Information System (INIS)

Ferreira Filho, L.G.; Nemes, M.C.

1986-02-01

It is shown that the inclusion of correlation effects in describing the nuclear photoabsorption cross section leads to a Breit-Wigner type curve with energy dependent width. It is also shown that a very slow energy dependence is enough to reproduce the data up to ∼ 139 MeV. (Author) [pt

Thermally activated delayed fluorescence organic dots for two-photon fluorescence lifetime imaging

Science.gov (United States)

He, Tingchao; Ren, Can; Li, Zhuohua; Xiao, Shuyu; Li, Junzi; Lin, Xiaodong; Ye, Chuanxiang; Zhang, Junmin; Guo, Lihong; Hu, Wenbo; Chen, Rui

2018-05-01

Autofluorescence is a major challenge in complex tissue imaging when molecules present in the biological tissue compete with the fluorophore. This issue may be resolved by designing organic molecules with long fluorescence lifetimes. The present work reports the two-photon absorption (TPA) properties of a thermally activated delayed fluorescence (TADF) molecule with carbazole as the electron donor and dicyanobenzene as the electron acceptor (i.e., 4CzIPN). The results indicate that 4CzIPN exhibits a moderate TPA cross-section (˜9 × 10-50 cm4 s photon-1), high fluorescence quantum yield, and a long fluorescence lifetime (˜1.47 μs). 4CzIPN was compactly encapsulated into an amphiphilic copolymer via nanoprecipitation to achieve water-soluble organic dots. Interestingly, 4CzIPN organic dots have been utilized in applications involving two-photon fluorescence lifetime imaging (FLIM). Our work aptly demonstrates that TADF molecules are promising candidates of nonlinear optical probes for developing next-generation multiphoton FLIM applications.

Computational nanometrology of line-edge roughness: noise effects, cross-line correlations and the role of etch transfer

Science.gov (United States)

Constantoudis, Vassilios; Papavieros, George; Lorusso, Gian; Rutigliani, Vito; Van Roey, Frieda; Gogolides, Evangelos

2018-03-01

The aim of this paper is to investigate the role of etch transfer in two challenges of LER metrology raised by recent evolutions in lithography: the effects of SEM noise and the cross-line and edge correlations. The first comes from the ongoing scaling down of linewidths, which dictates SEM imaging with less scanning frames to reduce specimen damage and hence with more noise. During the last decade, it has been shown that image noise can be an important budget of the measured LER while systematically affects and alter the PSD curve of LER at high frequencies. A recent method for unbiased LER measurement is based on the systematic Fourier or correlation analysis to decompose the effects of noise from true LER (Fourier-Correlation filtering method). The success of the method depends on the PSD and HHCF curve. Previous experimental and model works have revealed that etch transfer affects the PSD of LER reducing its high frequency values. In this work, we estimate the noise contribution to the biased LER through PSD flat floor at high frequencies and relate it with the differences between the PSDs of lithography and etched LER. Based on this comparison, we propose an improvement of the PSD/HHCF-based method for noise-free LER measurement to include the missed high frequency real LER. The second issue is related with the increased density of lithographic patterns and the special characteristics of DSA and MP lithography patterns exhibits. In a previous work, we presented an enlarged LER characterization methodology for such patterns, which includes updated versions of the old metrics along with new metrics defined and developed to capture cross-edge and cross-line correlations. The fundamental concept has been the Line Center Roughness (LCR), the edge c-factor and the line c-factor correlation function and length quantifying the line fluctuations and the extent of cross-edge and cross-line correlations. In this work, we focus on the role of etch steps on cross-edge and

Multifractal detrended cross correlation analysis of neuro-degenerative diseases-An in depth study

Science.gov (United States)

Dutta, Srimonti; Ghosh, Dipak; Chatterjee, Sucharita

2018-02-01

This work revisits our previous study on human gait diseases, (Dutta et al., 2013) where we have studied the autocorrelation of human gait pattern in normal and diseased set. Significant difference in results was observed for normal and diseased set. However we were not able to distinguish between sets of Parkinson's and Huntington's disease. In this paper we attempt to study whether cross correlations between two feet of human gait pattern can help to distinguish between different diseased set. The results reveal that study of cross correlations can help to distinguish between Parkinson's and Huntington's disease.

Cross-correlation studies between CMB temperature anisotropies and 21 cm fluctuations

International Nuclear Information System (INIS)

Cooray, Asantha

2004-01-01

During the transition from a neutral to a fully reionized universe, scattering of cosmic microwave background (CMB) photons via free electrons leads to a new anisotropy contribution to the temperature distribution. If the reionization process is inhomogeneous and patchy, the era of reionization is also visible via brightness temperature fluctuations in the redshifted 21 cm line emission from neutral hydrogen. Since regions containing electrons and neutral hydrogen are expected to trace the same underlying density field, the two are (anti)correlated and this is expected to be reflected in the anisotropy maps via a correlation between arcminute-scale CMB temperature and the 21 cm background. In terms of the angular cross-power spectrum, unfortunately, this correlation is insignificant due to a geometric cancellation associated with second-order CMB anisotropies. The same cross correlation between ionized and neutral regions, however, can be studied using a bispectrum involving large-scale velocity field of ionized regions from the Doppler effect, arcminute-scale CMB anisotropies during reionization, and the 21 cm background. While the geometric cancellation is partly avoided, the signal-to-noise ratio related to this bispectrum is reduced due to the large cosmic variance related to velocity fluctuations traced by the Doppler effect. Unless the velocity field during reionization can be independently established, it is unlikely that the correlation information related to the relative distribution of ionized electrons and regions containing neutral hydrogen can be obtained with a combined study involving CMB and 21 cm fluctuations

Background Noise Reduction Using Adaptive Noise Cancellation Determined by the Cross-Correlation

Science.gov (United States)

Spalt, Taylor B.; Brooks, Thomas F.; Fuller, Christopher R.

2012-01-01

Background noise due to flow in wind tunnels contaminates desired data by decreasing the Signal-to-Noise Ratio. The use of Adaptive Noise Cancellation to remove background noise at measurement microphones is compromised when the reference sensor measures both background and desired noise. The technique proposed modifies the classical processing configuration based on the cross-correlation between the reference and primary microphone. Background noise attenuation is achieved using a cross-correlation sample width that encompasses only the background noise and a matched delay for the adaptive processing. A present limitation of the method is that a minimum time delay between the background noise and desired signal must exist in order for the correlated parts of the desired signal to be separated from the background noise in the crosscorrelation. A simulation yields primary signal recovery which can be predicted from the coherence of the background noise between the channels. Results are compared with two existing methods.

CCFpams: Atmospheric stellar parameters from cross-correlation functions

Science.gov (United States)

Malavolta, Luca; Lovis, Christophe; Pepe, Francesco; Sneden, Christopher; Udry, Stephane

2017-07-01

CCFpams allows the measurement of stellar temperature, metallicity and gravity within a few seconds and in a completely automated fashion. Rather than performing comparisons with spectral libraries, the technique is based on the determination of several cross-correlation functions (CCFs) obtained by including spectral features with different sensitivity to the photospheric parameters. Literature stellar parameters of high signal-to-noise (SNR) and high-resolution HARPS spectra of FGK Main Sequence stars are used to calibrate the stellar parameters as a function of CCF areas.

Multifractal detrended cross-correlations between crude oil market and Chinese ten sector stock markets

Science.gov (United States)

Yang, Liansheng; Zhu, Yingming; Wang, Yudong; Wang, Yiqi

2016-11-01

Based on the daily price data of spot prices of West Texas Intermediate (WTI) crude oil and ten CSI300 sector indices in China, we apply multifractal detrended cross-correlation analysis (MF-DCCA) method to investigate the cross-correlations between crude oil and Chinese sector stock markets. We find that the strength of multifractality between WTI crude oil and energy sector stock market is the highest, followed by the strength of multifractality between WTI crude oil and financial sector market, which reflects a close connection between energy and financial market. Then we do vector autoregression (VAR) analysis to capture the interdependencies among the multiple time series. By comparing the strength of multifractality for original data and residual errors of VAR model, we get a conclusion that vector auto-regression (VAR) model could not be used to describe the dynamics of the cross-correlations between WTI crude oil and the ten sector stock markets.

Up-Conversion Intersystem Crossing Rates in Organic Emitters for Thermally Activated Delayed Fluorescence: Impact of the Nature of Singlet vs Triplet Excited States

KAUST Repository

Samanta, Pralok Kumar; Kim, Dongwook; Coropceanu, Veaceslav; Bredas, Jean-Luc

2017-01-01

The rates for up-conversion intersystem crossing (UISC) from the T1 state to the S1 state are calculated for a series of organic emitters with an emphasis on thermally activated delayed fluorescence (TADF) materials. Both the spin-orbit coupling

Two-detector cross-correlation noise technique and its application in measuring reactor kinetic parameters

International Nuclear Information System (INIS)

Lu Guiping; Peng Feng; Yi Jieyi

1988-01-01

The two-detector cross-correlation noise technique is a new method of measuring reactor kinetic parameters developed in the sixties. It has the advantages of non-perturbation in core, high signal to noise ratio, low space dependent effect, and simple and reliable in measurement. A special set of cross-correlation analyzer has been prepared for measuring kinetic parameters of several reactor assemblies, such as the High Flux Engineering Test Reactor, its zero power mock up facility and a low enriched uranium light water lattice zero power facility

DARK MATTER SEARCHES IN THE GAMMA-RAY EXTRAGALACTIC BACKGROUND VIA CROSS-CORRELATIONS WITH GALAXY CATALOGS

International Nuclear Information System (INIS)

Cuoco, Alessandro; Regis, Marco; Fornengo, Nicolao; Xia, Jun-Qing; Branchini, Enzo; Viel, Matteo

2015-01-01

We compare the measured angular cross-correlation between the Fermi-Large Area Telescope γ-ray sky and catalogs of extragalactic objects with the expected signal induced by weakly interacting massive particle (WIMP) dark matter (DM). We include a detailed description of the contribution of astrophysical γ-ray emitters such as blazars, misaligned active galactic nucleus (AGN), and star-forming galaxies, and perform a global fit to the measured cross-correlation. Five catalogs are considered: Sloan Digital Sky Survey (SDSS)-DR6 quasars, Two Micron All Sky Survey galaxies, NRAO VLA Sky Survey radio galaxies, SDSS-DR8 Luminous Red Galaxies, and the SDSS-DR8 main galaxy sample. To model the cross-correlation signal, we use the halo occupation distribution formalism to estimate the number of galaxies of a given catalog in DM halos and their spatial correlation properties. We discuss uncertainties in the predicted cross-correlation signal arising from the DM clustering and WIMP microscopic properties, which set the DM γ-ray emission. The use of different catalogs probing objects at different redshifts significantly reduces, though not completely, the degeneracy among the different γ-ray components. We find that the presence of a significant WIMP DM signal is allowed by the data but not significantly preferred by the fit, although this is mainly due to a degeneracy with the misaligned AGN component. With modest substructure boost, the sensitivity of this method excludes thermal annihilation cross sections at 95% level for WIMP masses up to few tens of GeV. Constraining the low-redshift properties of astrophysical populations with future data will further improve the sensitivity to DM

Dark Matter Searches in the Gamma-ray Extragalactic Background via Cross-correlations with Galaxy Catalogs

Science.gov (United States)

Cuoco, Alessandro; Xia, Jun-Qing; Regis, Marco; Branchini, Enzo; Fornengo, Nicolao; Viel, Matteo

2015-12-01

We compare the measured angular cross-correlation between the Fermi-Large Area Telescope γ-ray sky and catalogs of extragalactic objects with the expected signal induced by weakly interacting massive particle (WIMP) dark matter (DM). We include a detailed description of the contribution of astrophysical γ-ray emitters such as blazars, misaligned active galactic nucleus (AGN), and star-forming galaxies, and perform a global fit to the measured cross-correlation. Five catalogs are considered: Sloan Digital Sky Survey (SDSS)-DR6 quasars, Two Micron All Sky Survey galaxies, NRAO VLA Sky Survey radio galaxies, SDSS-DR8 Luminous Red Galaxies, and the SDSS-DR8 main galaxy sample. To model the cross-correlation signal, we use the halo occupation distribution formalism to estimate the number of galaxies of a given catalog in DM halos and their spatial correlation properties. We discuss uncertainties in the predicted cross-correlation signal arising from the DM clustering and WIMP microscopic properties, which set the DM γ-ray emission. The use of different catalogs probing objects at different redshifts significantly reduces, though not completely, the degeneracy among the different γ-ray components. We find that the presence of a significant WIMP DM signal is allowed by the data but not significantly preferred by the fit, although this is mainly due to a degeneracy with the misaligned AGN component. With modest substructure boost, the sensitivity of this method excludes thermal annihilation cross sections at 95% level for WIMP masses up to few tens of GeV. Constraining the low-redshift properties of astrophysical populations with future data will further improve the sensitivity to DM.

DARK MATTER SEARCHES IN THE GAMMA-RAY EXTRAGALACTIC BACKGROUND VIA CROSS-CORRELATIONS WITH GALAXY CATALOGS

Energy Technology Data Exchange (ETDEWEB)

Cuoco, Alessandro; Regis, Marco; Fornengo, Nicolao [Dipartimento di Fisica, Università di Torino, via P. Giuria 1, I-10125 Torino (Italy); Xia, Jun-Qing [Department of Astronomy, Beijing Normal University, Beijing 100875 (China); Branchini, Enzo [Dipartimento di Matematica e Fisica, Università degli Studi “Roma Tre,” via della Vasca Navale 84, I-00146 Roma (Italy); Viel, Matteo, E-mail: cuoco@to.infn.it, E-mail: regis@to.infn.it, E-mail: fornengo@to.infn.it, E-mail: xiajq@bnu.edu.cn, E-mail: branchin@fis.uniroma3.it, E-mail: viel@oats.inaf.it [INAF Osservatorio Astronomico di Trieste, Via G. B. Tiepolo 11, I-34141, Trieste (Italy)

2015-12-15

We compare the measured angular cross-correlation between the Fermi-Large Area Telescope γ-ray sky and catalogs of extragalactic objects with the expected signal induced by weakly interacting massive particle (WIMP) dark matter (DM). We include a detailed description of the contribution of astrophysical γ-ray emitters such as blazars, misaligned active galactic nucleus (AGN), and star-forming galaxies, and perform a global fit to the measured cross-correlation. Five catalogs are considered: Sloan Digital Sky Survey (SDSS)-DR6 quasars, Two Micron All Sky Survey galaxies, NRAO VLA Sky Survey radio galaxies, SDSS-DR8 Luminous Red Galaxies, and the SDSS-DR8 main galaxy sample. To model the cross-correlation signal, we use the halo occupation distribution formalism to estimate the number of galaxies of a given catalog in DM halos and their spatial correlation properties. We discuss uncertainties in the predicted cross-correlation signal arising from the DM clustering and WIMP microscopic properties, which set the DM γ-ray emission. The use of different catalogs probing objects at different redshifts significantly reduces, though not completely, the degeneracy among the different γ-ray components. We find that the presence of a significant WIMP DM signal is allowed by the data but not significantly preferred by the fit, although this is mainly due to a degeneracy with the misaligned AGN component. With modest substructure boost, the sensitivity of this method excludes thermal annihilation cross sections at 95% level for WIMP masses up to few tens of GeV. Constraining the low-redshift properties of astrophysical populations with future data will further improve the sensitivity to DM.

Quantitative Studies of Antimicrobial Peptide Pore Formation in Large Unilamellar Vesicles by Fluorescence Correlation Spectroscopy (FCS)

DEFF Research Database (Denmark)

Kristensen, Kasper; Henriksen, Jonas Rosager; Andresen, Thomas Lars

2013-01-01

In spite of intensive research efforts over the past decades, the mechanisms by which membrane-active antimicrobial peptides interact with phospholipid membranes are not yet fully elucidated. New tools that can be used to characterize antimicrobial peptide-lipid membrane interactions are therefore...... to quantify leakage from large unilamellar vesicles is associated with a number of experimental pitfalls. Based on theoretical and experimental considerations, we discuss how to properly design experiments to avoid these pitfalls. Subsequently, we apply fluorescence correlation spectroscopy to quantify...

A novel coefficient for detecting and quantifying asymmetry of California electricity market based on asymmetric detrended cross-correlation analysis.

Science.gov (United States)

Wang, Fang

2016-06-01

In order to detect and quantify asymmetry of two time series, a novel cross-correlation coefficient is proposed based on recent asymmetric detrended cross-correlation analysis (A-DXA), which we called A-DXA coefficient. The A-DXA coefficient, as an important extension of DXA coefficient ρDXA, contains two directional asymmetric cross-correlated indexes, describing upwards and downwards asymmetric cross-correlations, respectively. By using the information of directional covariance function of two time series and directional variance function of each series itself instead of power-law between the covariance function and time scale, the proposed A-DXA coefficient can well detect asymmetry between the two series no matter whether the cross-correlation is significant or not. By means of the proposed A-DXA coefficient conducted over the asymmetry for California electricity market, we found that the asymmetry between the prices and loads is not significant for daily average data in 1999 yr market (before electricity crisis) but extremely significant for those in 2000 yr market (during the crisis). To further uncover the difference of asymmetry between the years 1999 and 2000, a modified H statistic (MH) and ΔMH statistic are proposed. One of the present contributions is that the high MH values calculated for hourly data exist in majority months in 2000 market. Another important conclusion is that the cross-correlation with downwards dominates over the whole 1999 yr in contrast to the cross-correlation with upwards dominates over the 2000 yr.

A novel coefficient for detecting and quantifying asymmetry of California electricity market based on asymmetric detrended cross-correlation analysis

Science.gov (United States)

Wang, Fang

2016-06-01

In order to detect and quantify asymmetry of two time series, a novel cross-correlation coefficient is proposed based on recent asymmetric detrended cross-correlation analysis (A-DXA), which we called A-DXA coefficient. The A-DXA coefficient, as an important extension of DXA coefficient ρ D X A , contains two directional asymmetric cross-correlated indexes, describing upwards and downwards asymmetric cross-correlations, respectively. By using the information of directional covariance function of two time series and directional variance function of each series itself instead of power-law between the covariance function and time scale, the proposed A-DXA coefficient can well detect asymmetry between the two series no matter whether the cross-correlation is significant or not. By means of the proposed A-DXA coefficient conducted over the asymmetry for California electricity market, we found that the asymmetry between the prices and loads is not significant for daily average data in 1999 yr market (before electricity crisis) but extremely significant for those in 2000 yr market (during the crisis). To further uncover the difference of asymmetry between the years 1999 and 2000, a modified H statistic (MH) and ΔMH statistic are proposed. One of the present contributions is that the high MH values calculated for hourly data exist in majority months in 2000 market. Another important conclusion is that the cross-correlation with downwards dominates over the whole 1999 yr in contrast to the cross-correlation with upwards dominates over the 2000 yr.

Uncertainty estimation of core safety parameters using cross-correlations of covariance matrix

International Nuclear Information System (INIS)

Yamamoto, Akio; Yasue, Yoshihiro; Endo, Tomohiro; Kodama, Yasuhiro; Ohoka, Yasunori; Tatsumi, Masahiro

2013-01-01

An uncertainty reduction method for core safety parameters, for which measurement values are not obtained, is proposed. We empirically recognize that there exist some correlations among the prediction errors of core safety parameters, e.g., a correlation between the control rod worth and the assembly relative power at corresponding position. Correlations of errors among core safety parameters are theoretically estimated using the covariance of cross sections and sensitivity coefficients of core parameters. The estimated correlations of errors among core safety parameters are verified through the direct Monte Carlo sampling method. Once the correlation of errors among core safety parameters is known, we can estimate the uncertainty of a safety parameter for which measurement value is not obtained. (author)

Cross-correlation Doppler global velocimetry (CC-DGV)

Science.gov (United States)

Cadel, Daniel R.; Lowe, K. Todd

2015-08-01

A flow velocimetry method, cross-correlation Doppler global velocimetry (CC-DGV), is presented as a robust, simplified, and high dynamic range implementation of the Doppler global/planar Doppler velocimetry technique. A sweep of several gigahertz of the vapor absorption spectrum is used for each velocity sample, with signals acquired from both Doppler-shifted scattered light within the flow and a non-Doppler shifted reference beam. Cross-correlation of these signals yields the Doppler shift between them, averaged over the duration of the scan. With presently available equipment, velocities from 0 ms-1 to over 3000 ms-1 can notionally be measured simultaneously, making the technique ideal for high speed flows. The processing routine is shown to be robust against large changes in the vapor pressure of the iodine cell, benefiting performance of the system in facilities where ambient conditions cannot be easily regulated. Validation of the system was performed with measurements of a model wind turbine blade boundary layer made in a 1.83 m by 1.83 m subsonic wind tunnel for which laser Doppler velocimetry (LDV) measurements were acquired alongside the CC-DGV results. CC-DGV uncertainties of ±1.30 ms-1, ±0.64 ms-1, and ±1.11 ms-1 were determined for the orthogonal stream-wise, transverse-horizontal, and transverse-vertical velocity components, and root-mean-square deviations of 2.77 ms-1 and 1.34 ms-1 from the LDV validation results were observed for Reynolds numbers of 1.5 million and 2 million, respectively. Volumetric mean velocity measurements are also presented for a supersonic jet, with velocity uncertainties of ±4.48 ms-1, ±16.93 ms-1, and ±0.50 ms-1 for the orthogonal components, and self-validation done by collapsing the data with a physical scaling.

Memory-induced sign reversals of the spatial cross-correlation for particles in viscoelastic shear flows

International Nuclear Information System (INIS)

Sauga, Ako; Laas, Katrin; Mankin, Romi

2015-01-01

Highlights: • Cross-correlation (CC) of coordinates of particles in viscoelastic shear flows is discussed. • Expressions for CC functions subjected to both internal and external noises are presented. • Impact of internal and external noises on CC functions are compared. • Memory-induced reentrant sign reversals of the spatial cross-moment are established. - Abstract: The behavior of shear-induced cross-correlation functions between particle fluctuations along orthogonal directions in the shear plane for harmonically trapped Brownian particles in a viscoelastic shear flow is studied. A generalized Langevin equation with a power-law-type memory kernel is used to model the complex structure of the viscoelastic media. Interaction with fluctuations of environmental parameters is modeled by a multiplicative white Gaussian noise, by an internal fractional Gaussian noise, and by an additive external white noise. It is shown that the presence of a memory has a profound effect on the behavior of the cross-correlation functions. Particularly, memory-induced reentrant sign reversals of the spatial cross-moment between orthogonal random displacements of a particle are established, i.e., an increase of the memory exponent can cause the sign reversal from positive to negative, but by a further increase of the memory exponent a reentrant transition from negative to positive values appears. Similarities and differences between the behavior of the models with additive internal and external noises are considered. It is shown that additive external and internal noises cause qualitatively different dependencies of the cross-correlation functions on the time lag. The occurrence of energetic instability due to the influence of multiplicative noise is also discussed.

Depth profile analysis of non-specific fluorescence and color of tooth tissues after peroxide bleaching.

Science.gov (United States)

Klukowska, Malgorzata; Götz, Hermann; White, Donald J; Zoladz, James; Schwarz, Björn-Olaf; Duschner, Heinz

2013-02-01

To examine laboratory changes of endogenous non-specific fluorescence and color throughout subsurface of tooth structures prior to and following peroxide bleaching. Extracted human teeth were cross sectioned and mounted on glass slides. Cross sections were examined for internal color (digital camera) and nonspecific fluorescence (microRaman spectroscopy) throughout the tooth structure at specified locations. Surfaces of sections were then saturation bleached for 70 hours with a gel containing 6% hydrogen peroxide. Cross sections were reexamined for color and non-specific fluorescence changes. Unbleached enamel, dentin-enamel junction and dentin exhibit different CIELab color and non-specific fluorescence properties. Bleaching of teeth produced significant changes in color of internal cross sections and substantial reductions of non-specific fluorescence levels within enamel dentin and DEJ. Enamel and dentin non-specific fluorescence were reduced to common values with bleaching with enamel and the DEJ showing larger reductions than dentin.

A quantitative correlation between the mobility and crystallinity of photo-cross-linkable P3HT

KAUST Repository

Woo, Claire; Piliego, Claudia; Holcombe, Thomas W.; Toney, Michael F.; Frechet, Jean

2012-01-01

The performance of polymer field effect transistors (FETs) can vary by orders of magnitude by applying different processing conditions. Although it is generally believed that a higher degree of crystallinity results in a higher mobility, the correlation is not straightforward. In addition, the effect of cross-linking on polymer thin film microstructural order is relatively unknown. This study investigates the effect of thermal annealing and UV-initiated photo-cross-linking on the FET performance and microstructural order of a photo-cross-linkable P3HT derivative. Our results demonstrate that while cross-linking did not disrupt the overall crystallinity of the polymer thin film, the photo-cross-linking process likely induced doping in the semiconductor layer, leading to the absence of saturation behavior in the FET. Annealing after cross-linking slightly improved the FET performance but only minimally affected the microstructural order of the polymer film since the 3D morphology had been "locked in" during the first cross-linking step. Importantly, annealing and cross-linking simultaneously was a successful method to preserve polymer crystallinity while also achieving effective cross-linking. Using newly developed quantitative X-ray analysis techniques, our study established a quantitative correlation between FET charge mobility and thin film crystallinity. © 2012 American Chemical Society.

A quantitative correlation between the mobility and crystallinity of photo-cross-linkable P3HT

KAUST Repository

Woo, Claire

2012-04-10

The performance of polymer field effect transistors (FETs) can vary by orders of magnitude by applying different processing conditions. Although it is generally believed that a higher degree of crystallinity results in a higher mobility, the correlation is not straightforward. In addition, the effect of cross-linking on polymer thin film microstructural order is relatively unknown. This study investigates the effect of thermal annealing and UV-initiated photo-cross-linking on the FET performance and microstructural order of a photo-cross-linkable P3HT derivative. Our results demonstrate that while cross-linking did not disrupt the overall crystallinity of the polymer thin film, the photo-cross-linking process likely induced doping in the semiconductor layer, leading to the absence of saturation behavior in the FET. Annealing after cross-linking slightly improved the FET performance but only minimally affected the microstructural order of the polymer film since the 3D morphology had been "locked in" during the first cross-linking step. Importantly, annealing and cross-linking simultaneously was a successful method to preserve polymer crystallinity while also achieving effective cross-linking. Using newly developed quantitative X-ray analysis techniques, our study established a quantitative correlation between FET charge mobility and thin film crystallinity. © 2012 American Chemical Society.

MEASUREMENT OF 21 cm BRIGHTNESS FLUCTUATIONS AT z ∼ 0.8 IN CROSS-CORRELATION

International Nuclear Information System (INIS)

Masui, K. W.; Switzer, E. R.; Calin, L.-M.; Pen, U.-L.; Shaw, J. R.; Banavar, N.; Bandura, K.; Blake, C.; Chang, T.-C.; Liao, Y.-W.; Chen, X.; Li, Y.-C.; Natarajan, A.; Peterson, J. B.; Voytek, T. C.

2013-01-01

In this Letter, 21 cm intensity maps acquired at the Green Bank Telescope are cross-correlated with large-scale structure traced by galaxies in the WiggleZ Dark Energy Survey. The data span the redshift range 0.6 HI b HI r = [0.43 ± 0.07(stat.) ± 0.04(sys.)] × 10 –3 , where Ω HI is the neutral hydrogen (H I) fraction, r is the galaxy-hydrogen correlation coefficient, and b HI is the H I bias parameter. This is the most precise constraint on neutral hydrogen density fluctuations in a challenging redshift range. Our measurement improves the previous 21 cm cross-correlation at z ∼ 0.8 both in its precision and in the range of scales probed.

A prototype of on-line digital flow rate meter based on cross-correlation principle

International Nuclear Information System (INIS)

Sun Xiaodong; Dai Zhenxi; Xu Jijun

1997-01-01

An on-line, digital prototype of flow rate measurement system based on cross-correlation principle is developed. Laboratory measurements using the prototype show that sufficiently large temperature fluctuations exist naturally and that measurements are possible. Temperature fluctuations are detected by two identical thermocouples spaced along the flow direction and are pre-processed by a thermocouple signal amplifier. The pre-processed temperature fluctuations are analyzed by a cross-correlator which measures the transit time of temperature fluctuations between two thermocouples directly. Thus, the so-called correlation velocity can be determined by a chip microprocessor 8031. Experimental results with single-phase under steady conditions also show that the distance between two thermocouples and the Reynolds number of fluid are the most important parameters to the measurement

The Cross-Correlation and Reshuffling Tests in Discerning Induced Seismicity

Science.gov (United States)

Schultz, Ryan; Telesca, Luciano

2018-05-01

In recent years, cases of newly emergent induced clusters have increased seismic hazard and risk in locations with social, environmental, and economic consequence. Thus, the need for a quantitative and robust means to discern induced seismicity has become a critical concern. This paper reviews a Matlab-based algorithm designed to quantify the statistical confidence between two time-series datasets. Similar to prior approaches, our method utilizes the cross-correlation to delineate the strength and lag of correlated signals. In addition, use of surrogate reshuffling tests allows for the dynamic testing against statistical confidence intervals of anticipated spurious correlations. We demonstrate the robust nature of our algorithm in a suite of synthetic tests to determine the limits of accurate signal detection in the presence of noise and sub-sampling. Overall, this routine has considerable merit in terms of delineating the strength of correlated signals, one of which includes the discernment of induced seismicity from natural.

The importance of parameter variances, correlations lengths, and cross-correlations in reactive transport models: key considerations for assessing the need for microscale information

Energy Technology Data Exchange (ETDEWEB)

Reimus, Paul W [Los Alamos National Laboratory

2010-12-08

A process-oriented modeling approach is implemented to examine the importance of parameter variances, correlation lengths, and especially cross-correlations in contaminant transport predictions over large scales. It is shown that the most important consideration is the correlation between flow rates and retardation processes (e.g., sorption, matrix diffusion) in the system. lf flow rates are negatively correlated with retardation factors in systems containing multiple flow pathways, then characterizing these negative correlation(s) may have more impact on reactive transport modeling than microscale information. Such negative correlations are expected in porous-media systems where permeability is negatively correlated with clay content and rock alteration (which are usually associated with increased sorption). Likewise, negative correlations are expected in fractured rocks where permeability is positively correlated with fracture apertures, which in turn are negatively correlated with sorption and matrix diffusion. Parameter variances and correlation lengths are also shown to have important effects on reactive transport predictions, but they are less important than parameter cross-correlations. Microscale information pertaining to contaminant transport has become more readily available as characterization methods and spectroscopic instrumentation have achieved lower detection limits, greater resolution, and better precision. Obtaining detailed mechanistic insights into contaminant-rock-water interactions is becoming a routine practice in characterizing reactive transport processes in groundwater systems (almost necessary for high-profile publications). Unfortunately, a quantitative link between microscale information and flow and transport parameter distributions or cross-correlations has not yet been established. One reason for this is that quantitative microscale information is difficult to obtain in complex, heterogeneous systems. So simple systems that lack the

Uncertainty estimation of core safety parameters using cross-correlations of covariance matrix

International Nuclear Information System (INIS)

Yamamoto, A.; Yasue, Y.; Endo, T.; Kodama, Y.; Ohoka, Y.; Tatsumi, M.

2012-01-01

An uncertainty estimation method for core safety parameters, for which measurement values are not obtained, is proposed. We empirically recognize the correlations among the prediction errors among core safety parameters, e.g., a correlation between the control rod worth and assembly relative power of corresponding position. Correlations of uncertainties among core safety parameters are theoretically estimated using the covariance of cross sections and sensitivity coefficients for core parameters. The estimated correlations among core safety parameters are verified through the direct Monte-Carlo sampling method. Once the correlation of uncertainties among core safety parameters is known, we can estimate the uncertainty of a safety parameter for which measurement value is not obtained. Furthermore, the correlations can be also used for the reduction of uncertainties of core safety parameters. (authors)

Fluorescence Behavior and Dural Infiltration of Meningioma Analyzed by 5-Aminolevulinic Acid-Based Fluorescence: Operating Microscope Versus Mini-Spectrometer.

Science.gov (United States)

Knipps, Johannes; Beseoglu, Kerim; Kamp, Marcel; Fischer, Igor; Felsberg, Joerg; Neumann, Lisa M; Steiger, Hans-Jakob; Cornelius, Jan F

2017-12-01

To compare fluorescence intensity of tumor specimens, as measured by a fluorescence-guided surgery microscope and a spectrometer, to evaluate tumor infiltration of dura mater around meningiomas with help of these 2 different 5-aminolevulinic acid (5-ALA)-based fluorescence tools, and to correlate fluorescence intensity with histopathologic data. In a clinical series, meningiomas were resected by 5-ALA fluorescence-guided surgery. Fluorescence intensity was semiquantitatively rated by the surgeon at predefined points. Biopsies were harvested and fluorescence intensity measured by a spectrometer and histopathologically analyzed. Sampling was realized at the level of the dura in a centrifugal direction. A total of 104 biopsies (n = 13 tumors) were analyzed. Specificity and sensitivity of the microscope were 0.96 and 0.53 and of the spectrometer 0.95 and 0.93, respectively. Fluorescence intensity as measured by the spectrometer was correlated to histologically confirmed tumor burden. In a centrifugal direction, tumor burden and fluorescence intensity continuously decreased (along the dural tail). Below a threshold value of 639 arbitrary units no tumor was histologically detectable. At the level of the dura the spectrometer was highly sensitive for detection of meningioma cells. The surgical microscope showed false negative results and missed residual tumor cells in more than one half of the cases. The complementary use of both fluorescence tools may improve resection quality. Copyright © 2017 Elsevier Inc. All rights reserved.

Fluorescence lifetime imaging of skin cancer

Science.gov (United States)

Patalay, Rakesh; Talbot, Clifford; Munro, Ian; Breunig, Hans Georg; König, Karsten; Alexandrov, Yuri; Warren, Sean; Neil, Mark A. A.; French, Paul M. W.; Chu, Anthony; Stamp, Gordon W.; Dunsby, Chris

2011-03-01

Fluorescence intensity imaging and fluorescence lifetime imaging microscopy (FLIM) using two photon microscopy (TPM) have been used to study tissue autofluorescence in ex vivo skin cancer samples. A commercially available system (DermaInspect®) was modified to collect fluorescence intensity and lifetimes in two spectral channels using time correlated single photon counting and depth-resolved steady state measurements of the fluorescence emission spectrum. Uniquely, image segmentation has been used to allow fluorescence lifetimes to be calculated for each cell. An analysis of lifetime values obtained from a range of pigmented and non-pigmented lesions will be presented.

Quantifying the number of color centers in single fluorescent nanodiamonds by photon correlation spectroscopy and Monte Carlo simulation

International Nuclear Information System (INIS)

Hui, Y.Y.; Chang, Y.-R.; Lee, H.-Y.; Chang, H.-C.; Lim, T.-S.; Fann Wunshain

2009-01-01

The number of negatively charged nitrogen-vacancy centers (N-V) - in fluorescent nanodiamond (FND) has been determined by photon correlation spectroscopy and Monte Carlo simulations at the single particle level. By taking account of the random dipole orientation of the multiple (N-V) - fluorophores and simulating the probability distribution of their effective numbers (N e ), we found that the actual number (N a ) of the fluorophores is in linear correlation with N e , with correction factors of 1.8 and 1.2 in measurements using linearly and circularly polarized lights, respectively. We determined N a =8±1 for 28 nm FND particles prepared by 3 MeV proton irradiation

Automated vessel segmentation using cross-correlation and pooled covariance matrix analysis.

Science.gov (United States)

Du, Jiang; Karimi, Afshin; Wu, Yijing; Korosec, Frank R; Grist, Thomas M; Mistretta, Charles A

2011-04-01

Time-resolved contrast-enhanced magnetic resonance angiography (CE-MRA) provides contrast dynamics in the vasculature and allows vessel segmentation based on temporal correlation analysis. Here we present an automated vessel segmentation algorithm including automated generation of regions of interest (ROIs), cross-correlation and pooled sample covariance matrix analysis. The dynamic images are divided into multiple equal-sized regions. In each region, ROIs for artery, vein and background are generated using an iterative thresholding algorithm based on the contrast arrival time map and contrast enhancement map. Region-specific multi-feature cross-correlation analysis and pooled covariance matrix analysis are performed to calculate the Mahalanobis distances (MDs), which are used to automatically separate arteries from veins. This segmentation algorithm is applied to a dual-phase dynamic imaging acquisition scheme where low-resolution time-resolved images are acquired during the dynamic phase followed by high-frequency data acquisition at the steady-state phase. The segmented low-resolution arterial and venous images are then combined with the high-frequency data in k-space and inverse Fourier transformed to form the final segmented arterial and venous images. Results from volunteer and patient studies demonstrate the advantages of this automated vessel segmentation and dual phase data acquisition technique. Copyright © 2011 Elsevier Inc. All rights reserved.

Fluorescence studies on the aggregation behaviors of collagen modified with NHS-activated poly(γ-glutamic acid).

Science.gov (United States)

Zhang, Min; Yang, Junhui; Yang, Qili; Huang, Liulian; Wu, Hui; Chen, Lihui; Ding, Cuicui

2018-06-01

The poly(γ-glutamic acid)-NHS (γ-PGA-NHS) esters were used to endow collagen with both of excellent water-solubility and thermal stability via cross-linking reaction between γ-PGA-NHS and collagen. In the present work, the effect of γ-PGA-NHS on the aggregation of collagen molecules was studied by fluorescence techniques. The fluorescence emission spectra of pyrene in collagen solutions and the intrinsic fluorescence emission spectra of collagen suggested different effects of γ-PGA-NHS on collagen molecules: inhibiting aggregation below critical aggregation concentration (CAC) and promoting aggregation above CAC. The two-dimensional (2D) fluorescence correlation spectra indicated that the intermolecular hydrogen bonding and cross-linking between γ-PGA-NHS and collagen would influence the aggregation of collagen molecules. By the ultra-sensitive differential scanning calorimeter (VP-DSC), it was found that the main denaturational transition temperature (T m2 ) of modified collagen increased, while its calorimetric enthalpy changes (ΔH 2 ) decreased compared to those of native collagen, further indicating that the modification of γ-PGA-NHS influenced the aggregation of collagen molecules. The study provide useful information for the utilizing and or the processing of water-soluble collagen in aqueous solution in the fields such as cosmetics, health care products, tissue engineering and biomedical materials, etc. Copyright © 2018 Elsevier B.V. All rights reserved.

Whistler-mode signals: Group delay by cross correlation

International Nuclear Information System (INIS)

Thomson, N.R.

1975-01-01

Group travel times of 18.6 kHz whistler-mode signals from NLK, Seattle, to Wellington, New Zealand, are now being measured using the normal FSK transmissions. This is done using a mini-computer programmed to perform real-time cross correlations between two receivers: one receiver gets its signal from a whip aerial on which the ground wave (subionospheric mode) dominates, while the other gets its signal from a loop oriented for minimum ground wave. Group travel time can thus be measured continuously while there are whistler-mode signals present. Delays of 0.2--0.8 seconds have been found

Bottomside sinusoidal irregularities in the equatorial F region. II - Cross-correlation and spectral analysis

Science.gov (United States)

Cragin, B. L.; Hanson, W. B.; Mcclure, J. P.; Valladares, C. E.

1985-01-01

Equatorial bottomside sinusoidal (BSS) irregularities have been studied by applying techniques of cross-correlation and spectral analysis to the Atmosphere Explorer data set. The phase of the cross-correlations of the plasma number density is discussed and the two drift velocity components observed using the retarding potential analyzer and ion drift meter on the satellite are discussed. Morphology is addressed, presenting the geographical distributions of the occurrence of BSS events for the equinoxes and solstices. Physical processes including the ion Larmor flux, interhemispheric plasma flows, and variations in the lower F region Pedersen conductivity are invoked to explain the findings.

Three dimensional winds: A maximum cross-correlation application to elastic lidar data

Energy Technology Data Exchange (ETDEWEB)

Buttler, William Tillman [Univ. of Texas, Austin, TX (United States)

1996-05-01

Maximum cross-correlation techniques have been used with satellite data to estimate winds and sea surface velocities for several years. Los Alamos National Laboratory (LANL) is currently using a variation of the basic maximum cross-correlation technique, coupled with a deterministic application of a vector median filter, to measure transverse winds as a function of range and altitude from incoherent elastic backscatter lidar (light detection and ranging) data taken throughout large volumes within the atmospheric boundary layer. Hourly representations of three-dimensional wind fields, derived from elastic lidar data taken during an air-quality study performed in a region of complex terrain near Sunland Park, New Mexico, are presented and compared with results from an Environmental Protection Agency (EPA) approved laser doppler velocimeter. The wind fields showed persistent large scale eddies as well as general terrain-following winds in the Rio Grande valley.

Nanoscale protein diffusion by STED-based pair correlation analysis.

Directory of Open Access Journals (Sweden)

Paolo Bianchini

Full Text Available We describe for the first time the combination between cross-pair correlation function analysis (pair correlation analysis or pCF and stimulated emission depletion (STED to obtain diffusion maps at spatial resolution below the optical diffraction limit (super-resolution. Our approach was tested in systems characterized by high and low signal to noise ratio, i.e. Capsid Like Particles (CLPs bearing several (>100 active fluorescent proteins and monomeric fluorescent proteins transiently expressed in living Chinese Hamster Ovary cells, respectively. The latter system represents the usual condition encountered in living cell studies on fluorescent protein chimeras. Spatial resolution of STED-pCF was found to be about 110 nm, with a more than twofold improvement over conventional confocal acquisition. We successfully applied our method to highlight how the proximity to nuclear envelope affects the mobility features of proteins actively imported into the nucleus in living cells. Remarkably, STED-pCF unveiled the existence of local barriers to diffusion as well as the presence of a slow component at distances up to 500-700 nm from either sides of nuclear envelope. The mobility of this component is similar to that previously described for transport complexes. Remarkably, all these features were invisible in conventional confocal mode.

Electron detachment dissociation of fluorescently labeled sialylated oligosaccharides.

Science.gov (United States)

Zhou, Wen; Håkansson, Kristina

2011-12-01

We explored the application of electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry to fluorescently labeled sialylated oligosaccharides. Standard sialylated oligosaccharides and a sialylated N-linked glycan released from human transferrin were investigated. EDD yielded extensive glycosidic cleavages and cross-ring cleavages in all cases studied, consistently providing complementary structural information compared with infrared multiphoton dissociation. Neutral losses and satellite ions such as C-2H ions were also observed following EDD. In addition, we examined the influence of different fluorescent labels. The acidic label 2-aminobenzoic acid (2-AA) enhanced signal abundance in negative-ion mode. However, few cross-ring fragments were observed for 2-AA-labeled oligosaccharides. The neutral label 2-aminobenzamide (2-AB) resulted in more cross-ring cleavages compared with 2-AA-labeled species, but not as extensive fragmentation as for native oligosaccharides, likely resulting from altered negative charge locations from introduction of the fluorescent tag. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A method for the estimation of the significance of cross-correlations in unevenly sampled red-noise time series

Science.gov (United States)

Max-Moerbeck, W.; Richards, J. L.; Hovatta, T.; Pavlidou, V.; Pearson, T. J.; Readhead, A. C. S.

2014-11-01

We present a practical implementation of a Monte Carlo method to estimate the significance of cross-correlations in unevenly sampled time series of data, whose statistical properties are modelled with a simple power-law power spectral density. This implementation builds on published methods; we introduce a number of improvements in the normalization of the cross-correlation function estimate and a bootstrap method for estimating the significance of the cross-correlations. A closely related matter is the estimation of a model for the light curves, which is critical for the significance estimates. We present a graphical and quantitative demonstration that uses simulations to show how common it is to get high cross-correlations for unrelated light curves with steep power spectral densities. This demonstration highlights the dangers of interpreting them as signs of a physical connection. We show that by using interpolation and the Hanning sampling window function we are able to reduce the effects of red-noise leakage and to recover steep simple power-law power spectral densities. We also introduce the use of a Neyman construction for the estimation of the errors in the power-law index of the power spectral density. This method provides a consistent way to estimate the significance of cross-correlations in unevenly sampled time series of data.

Fractal approach towards power-law coherency to measure cross-correlations between time series

Czech Academy of Sciences Publication Activity Database

Krištoufek, Ladislav

2017-01-01

Roč. 50, č. 1 (2017), s. 193-200 ISSN 1007-5704 R&D Projects: GA ČR(CZ) GP14-11402P Institutional support: RVO:67985556 Keywords : power- law coherency * power- law cross-correlations * correlations Subject RIV: AH - Economics OBOR OECD: Applied Economics, Econometrics Impact factor: 2.784, year: 2016 http://library.utia.cas.cz/separaty/2017/E/kristoufek-0473066.pdf

Correlative Light-Electron Microscopy of Lipid-Encapsulated Fluorescent Nanodiamonds for Nanometric Localization of Cell Surface Antigens.

Science.gov (United States)

Hsieh, Feng-Jen; Chen, Yen-Wei; Huang, Yao-Kuan; Lee, Hsien-Ming; Lin, Chun-Hung; Chang, Huan-Cheng

2018-02-06

Containing an ensemble of nitrogen-vacancy centers in crystal matrices, fluorescent nanodiamonds (FNDs) are a new type of photostable markers that have found wide applications in light microscopy. The nanomaterial also has a dense carbon core, making it visible to electron microscopy. Here, we show that FNDs encapsulated in biotinylated lipids (bLs) are useful for subdiffraction imaging of antigens on cell surface with correlative light-electron microscopy (CLEM). The lipid encapsulation enables not only good dispersion of the particles in biological buffers but also high specific labeling of live cells. By employing the bL-encapsulated FNDs to target CD44 on HeLa cell surface through biotin-mediated immunostaining, we obtained the spatial distribution of these antigens by CLEM with a localization accuracy of ∼50 nm in routine operations. A comparative study with dual-color imaging, in which CD44 was labeled with FND and MICA/MICB was labeled with Alexa Fluor 488, demonstrated the superior performance of FNDs as fluorescent fiducial markers for CLEM of cell surface antigens.

Time-resolved fluorescence spectroscopy

International Nuclear Information System (INIS)

Gustavsson, Thomas; Mialocq, Jean-Claude

2007-01-01

This article addresses the evolution in time of light emitted by a molecular system after a brief photo-excitation. The authors first describe fluorescence from a photo-physical point of view and discuss the characterization of the excited state. Then, they explain some basic notions related to fluorescence characterization (lifetime and decays, quantum efficiency, so on). They present the different experimental methods and techniques currently used to study time-resolved fluorescence. They discuss basic notions of time resolution and spectral reconstruction. They briefly present some conventional methods: intensified Ccd cameras, photo-multipliers and photodiodes associated with a fast oscilloscope, and phase modulation. Other methods and techniques are more precisely presented: time-correlated single photon counting (principle, examples, and fluorescence lifetime imagery), streak camera (principle, examples), and optical methods like the Kerr optical effect (principle and examples) and fluorescence up-conversion (principle and theoretical considerations, examples of application)

Long memory of abnormal investor attention and the cross-correlations between abnormal investor attention and trading volume, volatility respectively

Science.gov (United States)

Fan, Xiaoqian; Yuan, Ying; Zhuang, Xintian; Jin, Xiu

2017-03-01

Taking Baidu Index as a proxy for abnormal investor attention (AIA), the long memory property in the AIA of Shanghai Stock Exchange (SSE) 50 Index component stocks was empirically investigated using detrended fluctuation analysis (DFA) method. The results show that abnormal investor attention is power-law correlated with Hurst exponents between 0.64 and 0.98. Furthermore, the cross-correlations between abnormal investor attention and trading volume, volatility respectively are studied using detrended cross-correlation analysis (DCCA) and the DCCA cross-correlation coefficient (ρDCCA). The results suggest that there are positive correlations between AIA and trading volume, volatility respectively. In addition, the correlations for trading volume are in general higher than the ones for volatility. By carrying on rescaled range analysis (R/S) and rolling windows analysis, we find that the results mentioned above are effective and significant.

Calibration of the Dodewaard downcomer thermocouple cross-correlation flow-rate measurements

Energy Technology Data Exchange (ETDEWEB)

Stekelenburg, A J.C. [Technische Univ. Delft (Netherlands). Interfacultair Reactor Inst.; Hagen, T.H.J.J. van der [Technische Univ. Delft (Netherlands). Interfacultair Reactor Inst.; Akker, H.E.A. van den [Technische Univ. Delft (Netherlands). Lab. voor Fysische Technologie

1992-12-01

The cross-correlation flow measurement technique, applied for measuring the coolant flow rate in a nuclear reactor, was calibrated with the use of numerical simulations of turbulent flow. The three-dimensional domain was collapsed into two dimensions. With a two-dimensional calculation of steady-state flow with transient thermal characteristics the response of thermocouples to a temperature variation was calculated. By cross-correlating the calculated thermocouple responses, the link between total flow rate and measured transit times was made. Three calibration points were taken in the range of 579 kg/s to 1477 kg/s. In this range, the product of the calculated transit time and the mass flow-rate is constant up to +3.5% and -2.4%. The reliability of the calibration was estimated at {+-}4.6%. The influence of the inlet boundary conditions, and the modelling of the flow in the upper part of the downcomer channel on the calibration result is shown to be small. A measured velocity profile effect was successfully predicted. (orig.).

Cross-correlation measurement of quantum shot noise using homemade transimpedance amplifiers

International Nuclear Information System (INIS)

Hashisaka, Masayuki; Ota, Tomoaki; Yamagishi, Masakazu; Fujisawa, Toshimasa; Muraki, Koji

2014-01-01

We report a cross-correlation measurement system, based on a new approach, which can be used to measure shot noise in a mesoscopic conductor at milliKelvin temperatures. In contrast to other measurement systems in which high-speed low-noise voltage amplifiers are commonly used, our system employs homemade transimpedance amplifiers (TAs). The low input impedance of the TAs significantly reduces the crosstalk caused by unavoidable parasitic capacitance between wires. The TAs are designed to have a flat gain over a frequency band from 2 kHz to 1 MHz. Low-noise performance is attained by installing the TAs at a 4 K stage of a dilution refrigerator. Our system thus fulfills the technical requirements for cross-correlation measurements: low noise floor, high frequency band, and negligible crosstalk between two signal lines. Using our system, shot noise generated at a quantum point contact embedded in a quantum Hall system is measured. The good agreement between the obtained shot-noise data and theoretical predictions demonstrates the accuracy of the measurements

Time dependence of entropy flux and entropy production for a dynamical system driven by noises with coloured cross-correlation

Institute of Scientific and Technical Information of China (English)

Xie Wen-Xian; Xu Wei; Cai Li

2007-01-01

This paper shows the Fokker-Planck equation of a dynamical system driven by coloured cross-correlated white noises in the absence and presence of a small external force. Based on the Fokker-Planck equation and the definition of Shannon's information entropy, the time dependence of entropy flux and entropy production can be calculated. The present results can be used to explain the extremal behaviour of time dependence of entropy flux and entropy production in view of the dissipative parameter γ of the system, coloured cross-correlation time τ and coloured cross-correlation strength λ.

Estimating phytoplankton photosynthesis by active fluorescence

Energy Technology Data Exchange (ETDEWEB)

Falkowski, P.G.; Kolber, Z.

1992-01-01

Photosynthesis can be described by target theory, At low photon flux densities, photosynthesis is a linear function of irradiance (I), The number of reaction centers (n), their effective absorption capture cross section {sigma}, and a quantum yield {phi}. As photosynthesis becomes increasingly light saturated, an increased fraction of reaction centers close. At light saturation the maximum photosynthetic rate is given as the product of the number of reaction centers (n) and their maximum electron transport rate (I/{tau}). Using active fluorometry it is possible to measure non-destructively and in real time the fraction of open or closed reaction centers under ambient irradiance conditions in situ, as well as {sigma} and {phi} {tau} can be readily, calculated from knowledge of the light saturation parameter, I{sub k} (which can be deduced by in situ by active fluorescence measurements) and {sigma}. We built a pump and probe fluorometer, which is interfaced with a CTD. The instrument measures the fluorescence yield of a weak probe flash preceding (f{sub 0}) and succeeding (f{sub 0}) a saturating pump flash. Profiles of the these fluorescence yields are used to derive the instantaneous rate of gross photosynthesis in natural phytoplankton communities without any incubation. Correlations with short-term simulated in situ radiocarbon measurements are extremely high. The average slope between photosynthesis derived from fluorescence and that measured by radiocarbon is 1.15 and corresponds to the average photosynthetic quotient. The intercept is about 15% of the maximum radiocarbon uptake and corresponds to the average net community respiration. Profiles of photosynthesis and sections showing the variability in its composite parameters reveal a significant effect of nutrient availability on biomass specific rates of photosynthesis in the ocean.

Estimating phytoplankton photosynthesis by active fluorescence

Energy Technology Data Exchange (ETDEWEB)

Falkowski, P.G.; Kolber, Z.

1992-10-01

Photosynthesis can be described by target theory, At low photon flux densities, photosynthesis is a linear function of irradiance (I), The number of reaction centers (n), their effective absorption capture cross section {sigma}, and a quantum yield {phi}. As photosynthesis becomes increasingly light saturated, an increased fraction of reaction centers close. At light saturation the maximum photosynthetic rate is given as the product of the number of reaction centers (n) and their maximum electron transport rate (I/{tau}). Using active fluorometry it is possible to measure non-destructively and in real time the fraction of open or closed reaction centers under ambient irradiance conditions in situ, as well as {sigma} and {phi} {tau} can be readily, calculated from knowledge of the light saturation parameter, I{sub k} (which can be deduced by in situ by active fluorescence measurements) and {sigma}. We built a pump and probe fluorometer, which is interfaced with a CTD. The instrument measures the fluorescence yield of a weak probe flash preceding (f{sub 0}) and succeeding (f{sub 0}) a saturating pump flash. Profiles of the these fluorescence yields are used to derive the instantaneous rate of gross photosynthesis in natural phytoplankton communities without any incubation. Correlations with short-term simulated in situ radiocarbon measurements are extremely high. The average slope between photosynthesis derived from fluorescence and that measured by radiocarbon is 1.15 and corresponds to the average photosynthetic quotient. The intercept is about 15% of the maximum radiocarbon uptake and corresponds to the average net community respiration. Profiles of photosynthesis and sections showing the variability in its composite parameters reveal a significant effect of nutrient availability on biomass specific rates of photosynthesis in the ocean.

Diverse Protocols for Correlative Super-Resolution Fluorescence Imaging and Electron Microscopy of Cells and Tissue

Science.gov (United States)

2016-05-25

polymerization36. Finally, we mutated surface residues on mEos2 to remove nucleophilic groups, which are involved in cross-linking with aldehydes and...Sites of Clathrin-Mediated Endocytosis. Current Biology 21, 1167– 1175 (2011). 49. Caplan, J. et al. Correlative Protein Localization in Yeast . (2013

Assessment of an X-Ray Spectrometer for fluorescence cross sections measurements of elements with 22≤Ζ≤55 in a secondary target configuration

International Nuclear Information System (INIS)

Delabat Diaz, Y.

2015-01-01

A performance evaluation of an X-Ray Fluorescence Spectrometer for X-Ray Fluorescence (XRF) cross section measurements in a Secondary Target (ST) set-up has been carried out. Using Cd and Dy as STs, an annular 241 Am (∼1 Ci) radioactive source and an X-Ray Spectrometer with a Si(Li) semiconductor detector, the photon effective flux factors (Ι 0 Gε) were measured for some elements with 22≤Ζ≤55 as a function of the characteristics X-Rays energy for two different distances Source-St (0.5 cm and 1.0 cm). Thin high purity foils and a few pellets made out of composed materials were used as samples for the Ι 0 Gε calibrations. the contribution of 59.54 KeV scattered photons to the XRF was analysed and the Scattering Correction Factor (SCF) due to excitation by 59.54 keV scattered photons was estimated in the Cd configuration for further cross section measurements improvements. (Author)

Cross-correlation of gravitational lensing from DES Science Verification data with SPT and Planck lensing

Energy Technology Data Exchange (ETDEWEB)

Kirk, D.; Omori, Y.; Benoit-Lévy, A.; Cawthon, R.; Chang, C.; Larsen, P.; Amara, A.; Bacon, D.; Crawford, T. M.; Dodelson, S.; Fosalba, P.; Giannantonio, T.; Holder, G.; Jain, B.; Kacprzak, T.; Lahav, O.; MacCrann, N.; Nicola, A.; Refregier, A.; Sheldon, E.; Story, K. T.; Troxel, M. A.; Vieira, J. D.; Vikram, V.; Zuntz, J.; Abbott, T. M. C.; Abdalla, F. B.; Becker, M. R.; Benson, B. A.; Bernstein, G. M.; Bernstein, R. A.; Bleem, L. E.; Bonnett, C.; Bridle, S. L.; Brooks, D.; Buckley-Geer, E.; Burke, D. L.; Capozzi, D.; Carlstrom, J. E.; Rosell, A. Carnero; Kind, M. Carrasco; Carretero, J.; Crocce, M.; Cunha, C. E.; D' Andrea, C. B.; da Costa, L. N.; Desai, S.; Diehl, H. T.; Dietrich, J. P.; Doel, P.; Eifler, T. F.; Evrard, A. E.; Flaugher, B.; Frieman, J.; Gerdes, D. W.; Goldstein, D. A.; Gruen, D.; Gruendl, R. A.; Honscheid, K.; James, D. J.; Jarvis, M.; Kent, S.; Kuehn, K.; Kuropatkin, N.; Lima, M.; March, M.; Martini, P.; Melchior, P.; Miller, C. J.; Miquel, R.; Nichol, R. C.; Ogando, R.; Plazas, A. A.; Reichardt, C. L.; Roodman, A.; Rozo, E.; Rykoff, E. S.; Sako, M.; Sanchez, E.; Scarpine, V.; Schubnell, M.; Sevilla-Noarbe, I.; Simard, G.; Smith, R. C.; Soares-Santos, M.; Sobreira, F.; Suchyta, E.; Swanson, M. E. C.; Tarle, G.; Thomas, D.; Wechsler, R. H.; Weller, J.

2016-03-10

We measure the cross-correlation between weak lensing of galaxy images and of the cosmic microwave background (CMB). The effects of gravitational lensing on different sources will be correlated if the lensing is caused by the same mass fluctuations. We use galaxy shape measurements from 139 deg$^{2}$ of the Dark Energy Survey (DES) Science Verification data and overlapping CMB lensing from the South Pole Telescope (SPT) and Planck. The DES source galaxies have a median redshift of $z_{\\rm med} {\\sim} 0.7$, while the CMB lensing kernel is broad and peaks at $z{\\sim}2$. The resulting cross-correlation is maximally sensitive to mass fluctuations at $z{\\sim}0.44$. Assuming the Planck 2015 best-fit cosmology, the amplitude of the DES$\\times$SPT cross-power is found to be $A = 0.88 \\pm 0.30$ and that from DES$\\times$Planck to be $A = 0.86 \\pm 0.39$, where $A=1$ corresponds to the theoretical prediction. These are consistent with the expected signal and correspond to significances of $2.9 \\sigma$ and $2.2 \\sigma$ respectively. We demonstrate that our results are robust to a number of important systematic effects including the shear measurement method, estimator choice, photometric redshift uncertainty and CMB lensing systematics. Significant intrinsic alignment of galaxy shapes would increase the cross-correlation signal inferred from the data; we calculate a value of $A = 1.08 \\pm 0.36$ for DES$\\times$SPT when we correct the observations with a simple IA model. With three measurements of this cross-correlation now existing in the literature, there is not yet reliable evidence for any deviation from the expected LCDM level of cross-correlation, given the size of the statistical uncertainties and the significant impact of systematic errors, particularly IAs. We provide forecasts for the expected signal-to-noise of the combination of the five-year DES survey and SPT-3G.

DCCA cross-correlation in blue-chips companies: A view of the 2008 financial crisis in the Eurozone

Science.gov (United States)

Guedes, E.; Dionísio, A.; Ferreira, P. J.; Zebende, G. F.

2017-08-01

In this paper we analyze the blue-chips (up to 50% of the total index) companies in the Eurozone. Our motivation being analysis of the effect of the 2008 financial crisis. For this purpose, we apply the DCCA cross-correlation coefficient (ρDCCA) between the country stock market index and their respective blue-chips. Then, with the cross-correlation coefficient, we qualify and quantify how each blue-chip is adherent to its country index, evaluating the type of cross-correlation among them. Subsequently, for each blue-chip, we propose to study the 2008 financial crisis by measuring the adherence between post and pre-crisis. From this analysis, we can construct an adhesion map of each company with respect to the global index. Our database is formed of 12 Eurozone countries.

Fluorescence fluctuation spectroscopy (FFS), part A

CERN Document Server

Tetin, Sergey

2013-01-01

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation SpectroscopyContains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers fluorescence fluctuation spectroscopyContains chapte

Silole-Based Red Fluorescent Organic Dots for Bright Two-Photon Fluorescence In vitro Cell and In vivo Blood Vessel Imaging.

Science.gov (United States)

Chen, Bin; Feng, Guangxue; He, Bairong; Goh, Chiching; Xu, Shidang; Ramos-Ortiz, Gabriel; Aparicio-Ixta, Laura; Zhou, Jian; Ng, Laiguan; Zhao, Zujin; Liu, Bin; Tang, Ben Zhong

2016-02-10

Robust luminescent dyes with efficient two-photon fluorescence are highly desirable for biological imaging applications, but those suitable for organic dots fabrication are still rare because of aggregation-caused quenching. In this work, a red fluorescent silole, 2,5-bis[5-(dimesitylboranyl)thiophen-2-yl]-1-methyl-1,3,4-triphenylsilole ((MesB)2 DTTPS), is synthesized and characterized. (MesB)2 DTTPS exhibits enhanced fluorescence efficiency in nanoaggregates, indicative of aggregation-enhanced emission (AEE). The organic dots fabricated by encapsulating (MesB)2 DTTPS within lipid-PEG show red fluorescence peaking at 598 nm and a high fluorescence quantum yield of 32%. Upon excitation at 820 nm, the dots show a large two-photon absorption cross section of 3.43 × 10(5) GM, which yields a two-photon action cross section of 1.09 × 10(5) GM. These (MesB)2 DTTPS dots show good biocompatibility and are successfully applied to one-photon and two-photon fluorescence imaging of MCF-7 cells and two-photon in vivo visualization of the blood vascular of mouse muscle in a high-contrast and noninvasive manner. Moreover, the 3D blood vasculature located at the mouse ear skin with a depth of over 100 μm can also be visualized clearly, providing the spatiotemporal information about the whole blood vascular network. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

RECONSTRUCTING REDSHIFT DISTRIBUTIONS WITH CROSS-CORRELATIONS: TESTS AND AN OPTIMIZED RECIPE

International Nuclear Information System (INIS)

Matthews, Daniel J.; Newman, Jeffrey A.

2010-01-01

Many of the cosmological tests to be performed by planned dark energy experiments will require extremely well-characterized photometric redshift measurements. Current estimates for cosmic shear are that the true mean redshift of the objects in each photo-z bin must be known to better than 0.002(1 + z), and the width of the bin must be known to ∼0.003(1 + z) if errors in cosmological measurements are not to be degraded significantly. A conventional approach is to calibrate these photometric redshifts with large sets of spectroscopic redshifts. However, at the depths probed by Stage III surveys (such as DES), let alone Stage IV (LSST, JDEM, and Euclid), existing large redshift samples have all been highly (25%-60%) incomplete, with a strong dependence of success rate on both redshift and galaxy properties. A powerful alternative approach is to exploit the clustering of galaxies to perform photometric redshift calibrations. Measuring the two-point angular cross-correlation between objects in some photometric redshift bin and objects with known spectroscopic redshift, as a function of the spectroscopic z, allows the true redshift distribution of a photometric sample to be reconstructed in detail, even if it includes objects too faint for spectroscopy or if spectroscopic samples are highly incomplete. We test this technique using mock DEEP2 Galaxy Redshift survey light cones constructed from the Millennium Simulation semi-analytic galaxy catalogs. From this realistic test, which incorporates the effects of galaxy bias evolution and cosmic variance, we find that the true redshift distribution of a photometric sample can, in fact, be determined accurately with cross-correlation techniques. We also compare the empirical error in the reconstruction of redshift distributions to previous analytic predictions, finding that additional components must be included in error budgets to match the simulation results. This extra error contribution is small for surveys that sample

Optimal portfolio strategy with cross-correlation matrix composed by DCCA coefficients: Evidence from the Chinese stock market

Science.gov (United States)

Sun, Xuelian; Liu, Zixian

2016-02-01

In this paper, a new estimator of correlation matrix is proposed, which is composed of the detrended cross-correlation coefficients (DCCA coefficients), to improve portfolio optimization. In contrast to Pearson's correlation coefficients (PCC), DCCA coefficients acquired by the detrended cross-correlation analysis (DCCA) method can describe the nonlinear correlation between assets, and can be decomposed in different time scales. These properties of DCCA make it possible to improve the investment effect and more valuable to investigate the scale behaviors of portfolios. The minimum variance portfolio (MVP) model and the Mean-Variance (MV) model are used to evaluate the effectiveness of this improvement. Stability analysis shows the effect of two kinds of correlation matrices on the estimation error of portfolio weights. The observed scale behaviors are significant to risk management and could be used to optimize the portfolio selection.

25 ns software correlator for photon and fluorescence correlation spectroscopy

Science.gov (United States)

Magatti, Davide; Ferri, Fabio

2003-02-01

A 25 ns time resolution, multi-tau software correlator developed in LABVIEW based on the use of a standard photon counting unit, a fast timer/counter board (6602-PCI National Instrument) and a personal computer (PC) (1.5 GHz Pentium 4) is presented and quantitatively discussed. The correlator works by processing the stream of incoming data in parallel according to two different algorithms: For large lag times (τ⩾100 μs), a classical time-mode (TM) scheme, based on the measure of the number of pulses per time interval, is used; differently, for τ⩽100 μs a photon-mode (PM) scheme is adopted and the time sequence of the arrival times of the photon pulses is measured. By combining the two methods, we developed a system capable of working out correlation functions on line, in full real time for the TM correlator and partially in batch processing for the PM correlator. For the latter one, the duty cycle depends on the count rate of the incoming pulses, being ˜100% for count rates ⩽3×104 Hz, ˜15% at 105 Hz, and ˜1% at 106 Hz. For limitations imposed by the fairly small first-in, first-out (FIFO) buffer available on the counter board, the maximum count rate permissible for a proper functioning of the PM correlator is limited to ˜105 Hz. However, this limit can be removed by using a board with a deeper FIFO. Similarly, the 25 ns time resolution is only limited by maximum clock frequency available on the 6602-PCI and can be easily improved by using a faster clock. When tested on dilute solutions of calibrated latex spheres, the overall performances of the correlator appear to be comparable with those of commercial hardware correlators, but with several nontrivial advantages related to its flexibility, low cost, and easy adaptability to future developments of PC and data acquisition technology.

Lead tolerance and cellular distribution in Elsholtzia splendens using synchrotron radiation micro-X-ray fluorescence

Energy Technology Data Exchange (ETDEWEB)

Zhang, Jie [MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China); Tian, Shengke [MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China); University of Florida, Institute of Food and Agricultural Science, Indian River Research and Education Center, Fort Pierce, FL 34945 (United States); Lu, Lingli; Shohag, M.J.I.; Liao, Haibing [MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China); Yang, Xiaoe, E-mail: xyang@zju.edu.cn [MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China)

2011-12-15

Highlights: Black-Right-Pointing-Pointer Elsholtzia splendens had a good ability of lead tolerance and accumulation. Black-Right-Pointing-Pointer Pb was mostly restricted to the vascular bundles and epidermis tissues. Black-Right-Pointing-Pointer Pb and Ca shared most similar distribution patterns in E. splendens. - Abstract: Hydroponic experiments were conducted to investigate the tolerance and spatial distribution of lead (Pb) in Elsholtzia splendens-a copper (Cu) accumulator plant using synchrotron-based micro-X-ray fluorescence. According to chlorophyll concentration and chlorophyll fluorescence parameters, E. splendens displayed certain tolerance at 100 {mu}M Pb treatment. Lead concentration in roots, stems and leaves of E. splendens reached 45,183.6, 1657.6, and 380.9 mg kg{sup -1}, respectively. Pb was mostly accumulated in the roots, and there were also high concentrations of Pb been transported into stems and leaves. Micro-XRF analysis of the stem and leaf cross section revealed that Pb was mostly restricted in the vascular bundles and epidermis tissues of both stem and leaf of E. splendens. The correlation between distribution of K, Ca, Zn and Pb were analyzed. There were significant positive correlations (P < 0.01) among Pb and Ca, K, Zn distribution both in stem and leaf of E. splendens. However, among the three elements, Ca shared the most similar distribution pattern and the highest correlation coefficients with Pb in both stem and leaf cross section of E. splendens. This suggests that Ca may play an important role in Pb accumulation in stem and leaf of E. splendens.

Inference of protein diffusion probed via fluorescence correlation spectroscopy

Science.gov (United States)

Tsekouras, Konstantinos

2015-03-01

Fluctuations are an inherent part of single molecule or few particle biophysical data sets. Traditionally, ``noise'' fluctuations have been viewed as a nuisance, to be eliminated or minimized. Here we look on how statistical inference methods - that take explicit advantage of fluctuations - have allowed us to draw an unexpected picture of single molecule diffusional dynamics. Our focus is on the diffusion of proteins probed using fluorescence correlation spectroscopy (FCS). First, we discuss how - in collaboration with the Bustamante and Marqusee labs at UC Berkeley - we determined using FCS data that individual enzymes are perturbed by self-generated catalytic heat (Riedel et al, Nature, 2014). Using the tools of inference, we found how distributions of enzyme diffusion coefficients shift in the presence of substrate revealing that enzymes performing highly exothermic reactions dissipate heat by transiently accelerating their center of mass following a catalytic reaction. Next, when molecules diffuse in the cell nucleus they often appear to diffuse anomalously. We analyze FCS data - in collaboration with Rich Day at the IU Med School - to propose a simple model for transcription factor binding-unbinding in the nucleus to show that it may give rise to apparent anomalous diffusion. Here inference methods extract entire binding affinity distributions for the diffusing transcription factors, allowing us to precisely characterize their interactions with different components of the nuclear environment. From this analysis, we draw key mechanistic insight that goes beyond what is possible by simply fitting data to ``anomalous diffusion'' models.

Electron Detachment Dissociation (EDD) of Fluorescently Labeled Sialylated Oligosaccharides

Science.gov (United States)

Zhou, Wen; Håkansson, Kristina

2012-01-01

We explored the application of electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry to fluorescently labeled sialylated oligosaccharides. Standard sialylated oligosaccharides and a sialylated N-linked glycan released from human transferrin were investigated. EDD yielded extensive glycosidic cleavages and cross-ring cleavages in all cases studied, consistently providing complementary structural information compared to IRMPD. Neutral losses and satellite ions such as C – 2H ions were also observed following EDD. In addition, we examined the influence of different fluorescent labels. The acidic label 2-aminobenzoic acid (2-AA) enhanced signal abundance in negative-ion mode. However, few cross-ring fragments were observed for 2-AA labeled oligosaccharides. The neutral label 2-aminobenzamide (2-AB) resulted in more cross-ring cleavages compared to 2-AA labeled species, but not as extensive fragmentation as for native oligosaccharides, likely resulting from altered negative charge locations from introduction of the fluorescent tag. PMID:22120881

Coordination analysis of players' distribution in football using cross-correlation and vector coding techniques.

Science.gov (United States)

Moura, Felipe Arruda; van Emmerik, Richard E A; Santana, Juliana Exel; Martins, Luiz Eduardo Barreto; Barros, Ricardo Machado Leite de; Cunha, Sergio Augusto

2016-12-01

The purpose of this study was to investigate the coordination between teams spread during football matches using cross-correlation and vector coding techniques. Using a video-based tracking system, we obtained the trajectories of 257 players during 10 matches. Team spread was calculated as functions of time. For a general coordination description, we calculated the cross-correlation between the signals. Vector coding was used to identify the coordination patterns between teams during offensive sequences that ended in shots on goal or defensive tackles. Cross-correlation showed that opponent teams have a tendency to present in-phase coordination, with a short time lag. During offensive sequences, vector coding results showed that, although in-phase coordination dominated, other patterns were observed. We verified that during the early stages, offensive sequences ending in shots on goal present greater anti-phase and attacking team phase periods, compared to sequences ending in tackles. Results suggest that the attacking team may seek to present a contrary behaviour of its opponent (or may lead the adversary behaviour) in the beginning of the attacking play, regarding to the distribution strategy, to increase the chances of a shot on goal. The techniques allowed detecting the coordination patterns between teams, providing additional information about football dynamics and players' interaction.

Cross-correlating the γ-ray Sky with Catalogs of Galaxy Clusters

Science.gov (United States)

Branchini, Enzo; Camera, Stefano; Cuoco, Alessandro; Fornengo, Nicolao; Regis, Marco; Viel, Matteo; Xia, Jun-Qing

2017-01-01

We report the detection of a cross-correlation signal between Fermi Large Area Telescope diffuse γ-ray maps and catalogs of clusters. In our analysis, we considered three different catalogs: WHL12, redMaPPer, and PlanckSZ. They all show a positive correlation with different amplitudes, related to the average mass of the objects in each catalog, which also sets the catalog bias. The signal detection is confirmed by the results of a stacking analysis. The cross-correlation signal extends to rather large angular scales, around 1°, that correspond, at the typical redshift of the clusters in these catalogs, to a few to tens of megaparsecs, I.e., the typical scale-length of the large-scale structures in the universe. Most likely this signal is contributed by the cumulative emission from active galactic nuclei (AGNs) associated with the filamentary structures that converge toward the high peaks of the matter density field in which galaxy clusters reside. In addition, our analysis reveals the presence of a second component, more compact in size and compatible with a point-like emission from within individual clusters. At present, we cannot distinguish between the two most likely interpretations for such a signal, I.e., whether it is produced by AGNs inside clusters or if it is a diffuse γ-ray emission from the intracluster medium. We argue that this latter, intriguing, hypothesis might be tested by applying this technique to a low-redshift large-mass cluster sample.

CROSS-CORRELATING THE γ -RAY SKY WITH CATALOGS OF GALAXY CLUSTERS

Energy Technology Data Exchange (ETDEWEB)

Branchini, Enzo [Dipartimento di Matematica e Fisica, Università degli Studi “Roma Tre,” via della Vasca Navale 84, I-00146 Roma (Italy); Camera, Stefano [Jodrell Bank Centre for Astrophysics, The University of Manchester, Alan Turing Building, Oxford Road, Manchester M13 9PL (United Kingdom); Cuoco, Alessandro [Institute for Theoretical Particle Physics and Cosmology, RWTH Aachen University, D-52056 Aachen (Germany); Fornengo, Nicolao; Regis, Marco [Dipartimento di Fisica, Università di Torino, via P. Giuria 1, I–10125 Torino (Italy); Viel, Matteo [INAF Osservatorio Astronomico di Trieste, Via G. B. Tiepolo 11, I-34141, Trieste (Italy); Xia, Jun-Qing, E-mail: xiajq@bnu.edu.cn, E-mail: branchin@fis.uniroma3.it, E-mail: stefano.camera@manchester.ac.uk, E-mail: cuoco@physik.rwth-aachen.de, E-mail: fornengo@to.infn.it, E-mail: regis@to.infn.it, E-mail: viel@oats.inaf.it [Department of Astronomy, Beijing Normal University, Beijing 100875 (China)

2017-01-01

We report the detection of a cross-correlation signal between Fermi Large Area Telescope diffuse γ -ray maps and catalogs of clusters. In our analysis, we considered three different catalogs: WHL12, redMaPPer, and PlanckSZ. They all show a positive correlation with different amplitudes, related to the average mass of the objects in each catalog, which also sets the catalog bias. The signal detection is confirmed by the results of a stacking analysis. The cross-correlation signal extends to rather large angular scales, around 1°, that correspond, at the typical redshift of the clusters in these catalogs, to a few to tens of megaparsecs, i.e., the typical scale-length of the large-scale structures in the universe. Most likely this signal is contributed by the cumulative emission from active galactic nuclei (AGNs) associated with the filamentary structures that converge toward the high peaks of the matter density field in which galaxy clusters reside. In addition, our analysis reveals the presence of a second component, more compact in size and compatible with a point-like emission from within individual clusters. At present, we cannot distinguish between the two most likely interpretations for such a signal, i.e., whether it is produced by AGNs inside clusters or if it is a diffuse γ -ray emission from the intracluster medium. We argue that this latter, intriguing, hypothesis might be tested by applying this technique to a low-redshift large-mass cluster sample.

Cross correlation analysis of medium energy gamma rays for the northern hemisphere

International Nuclear Information System (INIS)

Long, J.; Zanrosso, E.; Zych, A.D.; White, R.S.

1982-01-01

Data obtained with the UCR gamma telescope have been analyzed using the cross-correlation method. The observations extended over 37.5 hr from 0930 UT, 30 Sept. to 2300 UT, 1 oct. 1978 at 32deg N. Lat. (Palestine, Texas). The Crab Nebula- Anticenter region was observed on consecutive days. The telescope's wide field-of-view permitted the search for a number of other medium energy (1-30 MeV) source candidates. As the telescope swept the sky, the count rates for fixed celestial directions were correlated with the expected response as a function of time and telescope geometry. Similar correlations were carried out for sources measured in the laboratory and computer-simulated sources. In the correlation method the time independence and azimuthal symmetry of the atmospheric and cosmic diffuse backgrounds provide zero correlation. In contrast, a celestial source produces an asymmetric response with respect to the azimuthal direction which varies predictably in time to give a positive correlation. Preliminary correlation skymaps of the Anticenter region are presented and their statistical significance discussed. An energy spectrum obtained from the ''correlated counts'' is compared with measurements by other methods

Improved dark energy detection through the polarization-assisted cross correlation of the cosmic microwave background with radio sources

International Nuclear Information System (INIS)

Liu, Guo-Chin; Ng, Kin-Wang; Pen, Ue-Li

2011-01-01

Integrated Sachs-Wolfe (ISW) effect can be estimated by cross-correlating the cosmic microwave background (CMB) sky with tracers of the local matter distribution. At late cosmic time, the dark energy-induced decay of gravitation potential generates a cross correlation signal on large angular scales. The dominant noise is the intrinsic CMB anisotropies from the inflationary epoch. In this paper we use CMB polarization to reduce this intrinsic noise. We cross-correlate the microwave sky observed by Wilkinson Microwave Anisotropy Probe (WMAP) with the radio source catalog compiled by NRAO VLA Sky Survey (NVSS) to study the efficiency of the noise suppression. We find that the error bars are reduced by about 4 to 14% and the statistical power in the signal is improved.

On the determination of neutrino masses and dark energy evolution from the cross-correlation of CMB and LSS

International Nuclear Information System (INIS)

Ichikawa, Kazuhide; Takahashi, Tomo

2008-01-01

We discuss the possibilities of the simultaneous determination of the neutrino masses and the evolution of dark energy from future cosmological observations such as cosmic microwave background (CMB), large scale structure (LSS) and the cross-correlation between them. Recently it has been discussed that there is a degeneracy between the neutrino masses and the equation of state for dark energy. It is also known that there are some degeneracies among the parameters describing the dark energy evolution. We discuss the implications of these for the cross-correlation of CMB with LSS in some detail. Then we consider to what extent we can determine the neutrino masses and the dark energy evolution using the expected data from CMB, LSS and their cross-correlation

Application of the X-ray fluorescence analysis and X-ray diffraction in geochemical studies of the Pleistocene tills from Holy Cross Mountains

International Nuclear Information System (INIS)

Kubala-Kukuś, A.; Ludwikowska-Kedzia, M.; Banaś, D.; Braziewicz, J.; Majewska, U.; Pajek, M.; Wudarczyk-Moćko, J.

2013-01-01

X-ray fluorescence analysis methods (wavelength dispersive X-ray fluorescence analysis (WDXRF) and total reflection X-ray fluorescence (TXRF)) and X-ray powder diffraction (XRPD) have been applied in complementary geochemical studies of the Pleistocene till samples. The XRPD technique gave information about the mineral composition of the analyzed samples while the WDXRF and TXRF studies allowed the fast elemental analysis. The till samples were collected from different regions of Holy Cross Mountains (located in central Poland) which are still not unambiguously described in the context of the geochemical studies of the Quaternary sediments. The analysis was concentrated on the geochemical composition of the till samples both for materials occurring on the surface (characterized by continuous weathering processes) and for samples taken from core borehole. The overriding purpose of these studies is determination of the local lithotype of the tills and its lithologic and petrographic diagnostic properties, including the chemical composition of clay and minerals found in the clay. In the presented work the experimental sets up, sample preparation procedure and measurements programme will be discussed in details. Finally, the elemental and mineral compositions will be presented for studied different groups of the samples. - Highlights: • XRF analysis and X-ray diffraction used in studies of the till samples. • The till samples were collected from different regions of Holy Cross Mountains. • The analysis concentrates both on the samples from surface and from core borehole. • The purpose is determination of the local lithotype of the tills. • The experimental setup, sample preparation, measurements and results are discussed

Confocal fluorescence techniques in industrial application

Science.gov (United States)

Eggeling, Christian; Gall, Karsten; Palo, Kaupo; Kask, Peet; Brand, Leif

2003-06-01

The FCS+plus family of evaluation tools for confocal fluorescence spectroscopy, which was developed during recent years, offers a comprehensive view to a series of fluorescence properties. Originating in fluorescence correlation spectroscopy (FCS) and using similar experimental equipment, a system of signal processing methods such as fluorescence intensity distribution analysis (FIDA) was created to analyze in detail the fluctuation behavior of fluorescent particles within a small area of detection. Giving simultaneous access to molecular parameters like concentration, translational and rotational diffusion, molecular brightness, and multicolor coincidence, this portfolio was enhanced by more traditional techniques of fluorescence lifetime as well as time-resolved anisotropy determination. The cornerstones of the FCS+plus methodology will be shortly described. The inhibition of a phosphatase enzyme activity gives a comprehensive industrial application that demonstrates FCS+plus' versatility and its potential for pharmaceutical drug discovery.

Monitoring the diffusion behavior of Na,K-ATPase by fluorescence correlation spectroscopy (FCS) upon fluorescence labelling with eGFP or Dreiklang

Science.gov (United States)

Junghans, Cornelia; Schmitt, Franz-Josef; Vukojević, Vladana; Friedrich, Thomas

2016-02-01

Measurement of lateral mobility of membraneembedded proteins in living cells with high spatial and temporal precision is a challenging task of optofluidics. Biological membranes are complex structures, whose physico-chemical properties depend on the local lipid composition, cholesterol content and the presence of integral or peripheral membrane proteins, which may be involved in supramolecular complexes or are linked to cellular matrix proteins or the cytoskeleton. The high proteinto- lipid ratios in biomembranes indicate that membrane proteins are particularly subject to molecular crowding, making it difficult to follow the track of individual molecules carrying a fluorescence label. Novel switchable fluorescence proteins such as Dreiklang [1], are, in principle, promising tools to study the diffusion behavior of individual molecules in situations of molecular crowding due to excellent spectral control of the ON- and OFF-switching process. In this work, we expressed an integral membrane transport protein, the Na,K-ATPase comprising the human α2-subunit carrying an N-terminal eGFP or Dreiklang tag and human β1-subunit, in HEK293T cells and measured autocorrelation curves by fluorescence correlation spectroscopy (FCS). Furthermore,we measured diffusion times and diffusion constants of eGFP and Dreiklang by FCS, first, in aqueous solution after purification of the proteins upon expression in E. coli, and, second, upon expression as soluble proteins in the cytoplasm of HEK293T cells. Our data show that the diffusion behavior of the purified eGFP and Dreiklang in solution as well as the properties of the proteins expressed in the cytoplasm are very similar. However, the autocorrelation curves of eGFP- and Dreiklanglabeled Na,K-ATPase measured in the plasma membrane exhibit marked differences, with the Dreiklang-labeled construct showing shorter diffusion times. This may be related to an additional, as yet unrecognized quenching process that occurs on the same time

Intracellular localization and dynamics of Hypericin loaded PLLA nanocarriers by image correlation spectroscopy.

Science.gov (United States)

Penjweini, Rozhin; Deville, Sarah; D'Olieslaeger, Lien; Berden, Mandy; Ameloot, Marcel; Ethirajan, Anitha

2015-11-28

The study of cell-nanoparticle interactions is an important aspect for understanding drug delivery using nanocarriers. In this regard, advances in fluorescence based microscopy are useful for the investigation of temporal and spatial behavior of nanoparticles (NPs) within the intracellular environment. In this work, we focus on the delivery of the naturally-occurring hydrophobic photosensitizer Hypericin in human lung carcinoma A549 cells by using biodegradable poly L-lactic acid NPs. For the first time, Hypericin containing NPs are prepared by combining the miniemulsion technique with the solvent evaporation method. This approach yields an efficient loading of the NPs with Hypericin and allows for additional cargo molecules. To monitor the release of Hypercin from the NPs, an additional fluorescent lipophilic dye Coumarin-6 is incorporated in the NPs. Temporal and spatiotemporal image correlation spectroscopy is used to determine the fate of the NPs carrying the potential cargo. Both directed and non-directed motions are detected. By using image cross-correlation spectroscopy and specific fluorescent labeling of endosomes, lysosomes and mitochondria, the dynamics of the cargo loaded NPs in association with the organelles is studied. Copyright © 2015 Elsevier B.V. All rights reserved.

Real-time autocorrelator for fluorescence correlation spectroscopy based on graphical-processor-unit architecture: method, implementation, and comparative studies

Science.gov (United States)

Laracuente, Nicholas; Grossman, Carl

2013-03-01

We developed an algorithm and software to calculate autocorrelation functions from real-time photon-counting data using the fast, parallel capabilities of graphical processor units (GPUs). Recent developments in hardware and software have allowed for general purpose computing with inexpensive GPU hardware. These devices are more suited for emulating hardware autocorrelators than traditional CPU-based software applications by emphasizing parallel throughput over sequential speed. Incoming data are binned in a standard multi-tau scheme with configurable points-per-bin size and are mapped into a GPU memory pattern to reduce time-expensive memory access. Applications include dynamic light scattering (DLS) and fluorescence correlation spectroscopy (FCS) experiments. We ran the software on a 64-core graphics pci card in a 3.2 GHz Intel i5 CPU based computer running Linux. FCS measurements were made on Alexa-546 and Texas Red dyes in a standard buffer (PBS). Software correlations were compared to hardware correlator measurements on the same signals. Supported by HHMI and Swarthmore College

Effect of cross-correlation on track-to-track fusion

Science.gov (United States)

Saha, Rajat K.

1994-07-01

Since the advent of target tracking systems employing a diverse mixture of sensors, there has been increasing recognition by air defense system planners and other military system analysts of the need to integrate these tracks so that a clear air picture can be obtained in a command center. A popular methodology to achieve this goal is to perform track-to-track fusion, which performs track-to-track association as well as kinematic state vector fusion. This paper seeks to answer analytically the extent of improvement achievable by means of kinetic state vector fusion when the tracks are obtained from dissimilar sensors (e.g., Radar/ESM/IRST/IFF). It is well known that evaluation of the performance of state vector fusion algorithms at steady state must take into account the effects of cross-correlation between eligible tracks introduced by the input noise which, unfortunately, is often neglected because of added computational complexity. In this paper, an expression for the steady-state cross-covariance matrix for a 2D state vector track-to-track fusion is obtained. This matrix is shown to be a function of the parameters of the Kalman filters associated with the candidate tracks being fused. Conditions for positive definiteness of the cross-covariance matrix have been derived and the effect of positive definiteness on performance of track-to-track fusion is also discussed.

Principles of femtosecond X-ray/optical cross-correlation with X-ray induced transient optical reflectivity in solids

Energy Technology Data Exchange (ETDEWEB)

Eckert, S., E-mail: sebastian.eckert@helmholtz-berlin.de, E-mail: martin.beye@helmholtz-berlin.de; Beye, M., E-mail: sebastian.eckert@helmholtz-berlin.de, E-mail: martin.beye@helmholtz-berlin.de; Pietzsch, A.; Quevedo, W.; Hantschmann, M. [Institute for Methods and Instrumentation in Synchrotron Radiation Research, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin (Germany); Ochmann, M.; Huse, N. [Institute for Nanostructure and Solid State Physics, University of Hamburg, Jungiusstr. 11, 20355 Hamburg, Germany and Max Planck Institute for the Structure and Dynamics of Matter, Center for Free-Electron Laser Science, Luruper Chaussee 149, 22761 Hamburg (Germany); Ross, M.; Khalil, M. [Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195 (United States); Minitti, M. P.; Turner, J. J.; Moeller, S. P.; Schlotter, W. F.; Dakovski, G. L. [LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025 (United States); Föhlisch, A. [Institute for Methods and Instrumentation in Synchrotron Radiation Research, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin (Germany); Institut für Physik und Astronomie, Universität Potsdam, Karl-Liebknecht-Str. 24/25, 14476 Potsdam (Germany)

2015-02-09

The discovery of ultrafast X-ray induced optical reflectivity changes enabled the development of X-ray/optical cross correlation techniques at X-ray free electron lasers worldwide. We have now linked through experiment and theory the fundamental excitation and relaxation steps with the transient optical properties in finite solid samples. Therefore, we gain a thorough interpretation and an optimized detection scheme of X-ray induced changes to the refractive index and the X-ray/optical cross correlation response.

Novel Schiff base (DBDDP) selective detection of Fe (III): Dispersed in aqueous solution and encapsulated in silica cross-linked micellar nanoparticles in living cell.

Science.gov (United States)

Gai, Fangyuan; Yin, Li; Fan, Mengmeng; Li, Ling; Grahn, Johnny; Ao, Yuhui; Yang, Xudong; Wu, Xuming; Liu, Yunling; Huo, Qisheng

2018-03-15

This work demonstrated the synthesis of (4E)-4-(4-(diphenylamino)benzylideneamino)-1,2-dihydro-1,5- dimethyl-2-phenylpyrazol-3-one (DBDDP) for Fe (III) detection in aqueous media and in the core of silica cross-linked micellar nanoparticles in living cells. The free DBDDP performed fluorescence enhancement due to Fe (III)-promoted hydrolysis in a mixed aqueous solution, while the DBDDP-doped silica cross-linked micellar nanoparticles (DBDDP-SCMNPs) performed an electron-transfer based fluorescence quenching of Fe (III) in living cells. The quenching fluorescence of DBDDP-SCMNPs and the concentration of Fe (III) exhibited a linear correlation, which was in accordance with the Stern-Volmer equation. Moreover, DBDDP-SCMNPs showed a low limit of detection (LOD) of 0.1 ppm and an excellent selectivity against other metal ions. Due to the good solubility and biocompatibility, DBDDP-SCMNPs could be applied as fluorescence quenching nanosensors in living cells. Copyright © 2017 Elsevier Inc. All rights reserved.

Cross-Correlations in Quasar Radio Emission

Science.gov (United States)

Nefedyev, Yuri; Panischev, Oleg; Demin, Sergey

The main factors forming the complex evolution of the accretive astrophysical systems are nonlinearity, intermittency, nonstationarity and also collective phenomena. To discover the dynamic processes in these objects and to detain understanding their properties we need to use all the applicable analyzing methods. Here we use the Flicker-Noise Spectroscopy (FNS) as a phenomenological approach to analyzing and parameterizing the auto- and cross-correlations in time series of astrophysical objects dynamics. As an example we consider the quasar flux radio spectral density at frequencies 2.7 GHz and 8.1 GHz. Data have been observed by Dr. N. Tanizuka (Laboratory for Complex Systems Analysis, Osaka Prefecture University) in a period of 1979 to 1988 (3 309 days). According to mental habits quasar is a very energetic and distant active galactic nucleus containing a supermassive black hole by size 10-10,000 times the Schwarzschild radius. The quasar is powered by an accretion disc around the black hole. The accretion disc material layers, moving around the black hole, are under the influence of gravitational and frictional forces. It results in raising the high temperature and arising the resonant and collective phenomena reflected in quasar emission dynamics. Radio emission dynamics of the quasar 0215p015 is characterized by three quasi-periodic processes, which are prevalent in considering dynamics. By contrast the 1641p399's emission dynamics have not any distinguish processes. It means the presence of high intermittency in accretive modes. The second difference moment allows comparing the degree of manifesting of resonant and chaotic components in initial time series of the quasar radio emission. The comparative analysis shows the dominating of chaotic part of 1641p399's dynamics whereas the radio emission of 0215p015 has the predominance of resonant component. Analyzing the collective features of the quasar radio emission intensity demonstrates the significant

Cross-correlated relaxation rates between protein backbone H–X dipolar interactions

International Nuclear Information System (INIS)

Vögeli, Beat

2017-01-01

The relaxation interference between dipole–dipole interactions of two separate spin pairs carries structural and dynamics information. In particular, when compared to individual dynamic behavior of those spin pairs, such cross-correlated relaxation (CCR) rates report on the correlation between the spin pairs. We have recently mapped out correlated motion along the backbone of the protein GB3, using CCR rates among and between consecutive H N –N and H α –C α dipole–dipole interactions. Here, we provide a detailed account of the measurement of the four types of CCR rates. All rates were obtained from at least two different pulse sequences, of which the yet unpublished ones are presented. Detailed comparisons between the different methods and corrections for unwanted pathways demonstrate that the averaged CCR rates are highly accurate and precise with errors of 1.5–3% of the entire value ranges.

Cross-correlated relaxation rates between protein backbone H–X dipolar interactions

Energy Technology Data Exchange (ETDEWEB)

Vögeli, Beat, E-mail: beat.vogeli@ucdenver.edu [University of Colorado Denver, Department of Biochemistry and Molecular Genetics (United States)

2017-03-15

The relaxation interference between dipole–dipole interactions of two separate spin pairs carries structural and dynamics information. In particular, when compared to individual dynamic behavior of those spin pairs, such cross-correlated relaxation (CCR) rates report on the correlation between the spin pairs. We have recently mapped out correlated motion along the backbone of the protein GB3, using CCR rates among and between consecutive H{sup N}–N and H{sup α}–C{sup α} dipole–dipole interactions. Here, we provide a detailed account of the measurement of the four types of CCR rates. All rates were obtained from at least two different pulse sequences, of which the yet unpublished ones are presented. Detailed comparisons between the different methods and corrections for unwanted pathways demonstrate that the averaged CCR rates are highly accurate and precise with errors of 1.5–3% of the entire value ranges.

Determination of velocity vector angles using the directional cross-correlation method

DEFF Research Database (Denmark)

Kortbek, Jacob; Jensen, Jørgen Arendt

2005-01-01

and then select the angle with the highest normalized correlation between directional signals. The approach is investigated using Field II simulations and data from the experimental ultrasound scanner RASMUS and with a parabolic flow having a peak velocity of 0.3 m/s. A 7 MHz linear array transducer is used......A method for determining both velocity magnitude and angle in any direction is suggested. The method uses focusing along the velocity direction and cross-correlation for finding the correct velocity magnitude. The angle is found from beamforming directional signals in a number of directions......-time ) between signals to correlate, and a proper choice varies with flow angle and flow velocity. One performance example is given with a fixed value of k tprf for all flow angles. The angle estimation on measured data for flow at 60 ◦ to 90 ◦ , yields a probability of valid estimates between 68% and 98...

Determination of experimental K-shell fluorescence yield for ...

Indian Academy of Sciences (India)

X-ray; fluorescence yield; cross-section and chemical effects; potassium; cal- ... The X-ray production cross-section in Ti, V, Cr, Mn, Fe, Co, Ni and Cu .... where µinc (cm2 g−1) and µemt (cm2 g−1) are the mass attenuation coefficients at the.

Circumvention of fluorophore photobleaching in fluorescence fluctuation experiments: a beam scanning approach.

Science.gov (United States)

Satsoura, Dmitri; Leber, Brian; Andrews, David W; Fradin, Cécile

2007-04-23

Photobleaching is a fluorophore-damaging process that commonly afflicts single-molecule fluorescence studies. It becomes an especially severe problem in fluorescence fluctuation experiments when studying slowly diffusing particles. One way to circumvent this problem is to use beam scanning to decrease the residence time of the fluorophores in the excitation volume. We report a systematic study of the effects of circular beam scanning on the photobleaching of fluorescent particles as observed in single-photon excitation fluorescence fluctuation experiments. We start by deriving a simple expression relating the average detected fluorescence to the photobleaching cross section of the fluorophores. We then perform numerical calculations of the spatial distribution of fluorescent particles in order to understand under which conditions beam scanning can prevent the formation of a photobleaching hole. To support these predictions, we show experimental results obtained for large unilamellar vesicles containing a small amount of the fluorescent lipophilic tracer DiD. We establish the required scanning radius and frequency range in order to obtain sufficient reduction of the photobleaching effect for that system. From the detected increase in fluorescence upon increase in scanning speed, we estimate the photobleaching cross section of DiD.

Cross-correlation redshift calibration without spectroscopic calibration samples in DES Science Verification Data

Science.gov (United States)

Davis, C.; Rozo, E.; Roodman, A.; Alarcon, A.; Cawthon, R.; Gatti, M.; Lin, H.; Miquel, R.; Rykoff, E. S.; Troxel, M. A.; Vielzeuf, P.; Abbott, T. M. C.; Abdalla, F. B.; Allam, S.; Annis, J.; Bechtol, K.; Benoit-Lévy, A.; Bertin, E.; Brooks, D.; Buckley-Geer, E.; Burke, D. L.; Carnero Rosell, A.; Carrasco Kind, M.; Carretero, J.; Castander, F. J.; Crocce, M.; Cunha, C. E.; D'Andrea, C. B.; da Costa, L. N.; Desai, S.; Diehl, H. T.; Doel, P.; Drlica-Wagner, A.; Fausti Neto, A.; Flaugher, B.; Fosalba, P.; Frieman, J.; García-Bellido, J.; Gaztanaga, E.; Gerdes, D. W.; Giannantonio, T.; Gruen, D.; Gruendl, R. A.; Gutierrez, G.; Honscheid, K.; Jain, B.; James, D. J.; Jeltema, T.; Krause, E.; Kuehn, K.; Kuhlmann, S.; Kuropatkin, N.; Lahav, O.; Li, T. S.; Lima, M.; March, M.; Marshall, J. L.; Martini, P.; Melchior, P.; Ogando, R. L. C.; Plazas, A. A.; Romer, A. K.; Sanchez, E.; Scarpine, V.; Schindler, R.; Schubnell, M.; Sevilla-Noarbe, I.; Smith, M.; Soares-Santos, M.; Sobreira, F.; Suchyta, E.; Swanson, M. E. C.; Tarle, G.; Thomas, D.; Vikram, V.; Walker, A. R.; Wechsler, R. H.

2018-06-01

Galaxy cross-correlations with high-fidelity redshift samples hold the potential to precisely calibrate systematic photometric redshift uncertainties arising from the unavailability of complete and representative training and validation samples of galaxies. However, application of this technique in the Dark Energy Survey (DES) is hampered by the relatively low number density, small area, and modest redshift overlap between photometric and spectroscopic samples. We propose instead using photometric catalogues with reliable photometric redshifts for photo-z calibration via cross-correlations. We verify the viability of our proposal using redMaPPer clusters from the Sloan Digital Sky Survey (SDSS) to successfully recover the redshift distribution of SDSS spectroscopic galaxies. We demonstrate how to combine photo-z with cross-correlation data to calibrate photometric redshift biases while marginalizing over possible clustering bias evolution in either the calibration or unknown photometric samples. We apply our method to DES Science Verification (DES SV) data in order to constrain the photometric redshift distribution of a galaxy sample selected for weak lensing studies, constraining the mean of the tomographic redshift distributions to a statistical uncertainty of Δz ˜ ±0.01. We forecast that our proposal can, in principle, control photometric redshift uncertainties in DES weak lensing experiments at a level near the intrinsic statistical noise of the experiment over the range of redshifts where redMaPPer clusters are available. Our results provide strong motivation to launch a programme to fully characterize the systematic errors from bias evolution and photo-z shapes in our calibration procedure.

Correlative Stochastic Optical Reconstruction Microscopy and Electron Microscopy

Science.gov (United States)

Kim, Doory; Deerinck, Thomas J.; Sigal, Yaron M.; Babcock, Hazen P.; Ellisman, Mark H.; Zhuang, Xiaowei

2015-01-01

Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure. Recent development of super-resolution fluorescence microscopy allows the location of molecules to be determined with nanometer-scale spatial resolution. However, correlative super-resolution fluorescence microscopy and electron microscopy (EM) still remains challenging because the optimal specimen preparation and imaging conditions for super-resolution fluorescence microscopy and EM are often not compatible. Here, we have developed several experiment protocols for correlative stochastic optical reconstruction microscopy (STORM) and EM methods, both for un-embedded samples by applying EM-specific sample preparations after STORM imaging and for embedded and sectioned samples by optimizing the fluorescence under EM fixation, staining and embedding conditions. We demonstrated these methods using a variety of cellular targets. PMID:25874453

Isthmus Dependent Atrial Flutter Cycle Length Correlates with Right Atrial Cross-Sectional Area

Directory of Open Access Journals (Sweden)

Kousik Krishnan

2009-05-01

Full Text Available Background: Right atrial flutter cycle length can prolong in the presence of antiarrhythmic drug therapy. We hypothesized that the cycle length of right atrial isthmus dependent flutter would correlate with right atrial cross-sectional area measurements. Methods: 60 patients who underwent ablation for electrophysiologically proven isthmus dependent right atrial flutter, who were not on Class I or Class III antiarrhythmic drugs and had recent 2-dimensional echocardiographic data comprised the study group. Right atrial length and width were measured in the apical four chamber view. Cross-sectional area was estimated by multiplying the length and width. 35 patients had an atrial flutter rate ≥250 bpm (Normal Flutter Group and 25 patients had an atrial flutter rate < 250 bpm (Slow Flutter Group. Results: Mean atrial flutter rate was 283 bpm in the normal flutter group and 227 bpm in the slow flutter group. Mean atrial flutter cycle length was 213 ms in the Normal Flutter Group and 265 ms in the Slow Flutter Group (p<0.0001. Mean right atrial cross sectional area was 1845 mm2 in the Normal Flutter group and 2378 mm2 in the Slow Flutter Group, (p< 0.0001. Using linear regression, CSA was a significant predictor of cycle length (β =0.014 p = 0.0045. For every 1 mm2 increase in cross-sectional area, cycle length is 0.014 ms longer.Conclusion: In the absence of antiarrhythmic medications, right atrial cross sectional area enlargement correlates with atrial flutter cycle length. These findings provide further evidence that historical rate-related definitions of typical isthmus dependent right atrial are not mechanistically valid.

KiDS-450: tomographic cross-correlation of galaxy shear with Planck lensing

Science.gov (United States)

Harnois-Déraps, Joachim; Tröster, Tilman; Chisari, Nora Elisa; Heymans, Catherine; van Waerbeke, Ludovic; Asgari, Marika; Bilicki, Maciej; Choi, Ami; Erben, Thomas; Hildebrandt, Hendrik; Hoekstra, Henk; Joudaki, Shahab; Kuijken, Konrad; Merten, Julian; Miller, Lance; Robertson, Naomi; Schneider, Peter; Viola, Massimo

2017-10-01

We present the tomographic cross-correlation between galaxy lensing measured in the Kilo Degree Survey (KiDS-450) with overlapping lensing measurements of the cosmic microwave background (CMB), as detected by Planck 2015. We compare our joint probe measurement to the theoretical expectation for a flat Λ cold dark matter cosmology, assuming the best-fitting cosmological parameters from the KiDS-450 cosmic shear and Planck CMB analyses. We find that our results are consistent within 1σ with the KiDS-450 cosmology, with an amplitude re-scaling parameter AKiDS = 0.86 ± 0.19. Adopting a Planck cosmology, we find our results are consistent within 2σ, with APlanck = 0.68 ± 0.15. We show that the agreement is improved in both cases when the contamination to the signal by intrinsic galaxy alignments is accounted for, increasing A by ∼0.1. This is the first tomographic analysis of the galaxy lensing - CMB lensing cross-correlation signal, and is based on five photometric redshift bins. We use this measurement as an independent validation of the multiplicative shear calibration and of the calibrated source redshift distribution at high redshifts. We find that constraints on these two quantities are strongly correlated when obtained from this technique, which should therefore not be considered as a stand-alone competitive calibration tool.

Fluorescence guided surgery and tracer-dose, fact or fiction?

International Nuclear Information System (INIS)

KleinJan, Gijs H.; Olmos, Renato A.V.; Bunschoten, Anton; Berg, Nynke S. van den; Klop, W.M.C.; Horenblas, Simon; Poel, Henk G. van der; Wester, Hans-Juergen; Leeuwen, Fijs W.B. van

2016-01-01

Fluorescence guidance is an upcoming methodology to improve surgical accuracy. Challenging herein is the identification of the minimum dose at which the tracer can be detected with a clinical-grade fluorescence camera. Using a hybrid tracer such as indocyanine green (ICG)- 99m Tc-nanocolloid, it has become possible to determine the accumulation of tracer and correlate this to intraoperative fluorescence-based identification rates. In the current study, we determined the lower detection limit of tracer at which intraoperative fluorescence guidance was still feasible. Size exclusion chromatography (SEC) provided a laboratory set-up to analyze the chemical content and to simulate the migratory behavior of ICG-nanocolloid in tissue. Tracer accumulation and intraoperative fluorescence detection findings were derived from a retrospective analysis of 20 head-and-neck melanoma patients, 40 penile and 20 prostate cancer patients scheduled for sentinel node (SN) biopsy using ICG- 99m Tc-nanocolloid. In these patients, following tracer injection, single photon emission computed tomography fused with computed tomography (SPECT/CT) was used to identify the SN(s). The percentage injected dose (% ID), the amount of ICG (in nmol), and the concentration of ICG in the SNs (in μM) was assessed for SNs detected on SPECT/CT and correlated with the intraoperative fluorescence imaging findings. SEC determined that in the hybrid tracer formulation, 41 % (standard deviation: 12 %) of ICG was present in nanocolloid-bound form. In the SNs detected using fluorescence guidance a median of 0.88 % ID was present, compared to a median of 0.25 % ID in the non-fluorescent SNs (p-value < 0.001). The % ID values could be correlated to the amount ICG in a SN (range: 0.003-10.8 nmol) and the concentration of ICG in a SN (range: 0.006-64.6 μM). The ability to provide intraoperative fluorescence guidance is dependent on the amount and concentration of the fluorescent dye accumulated in the lesion(s) of

Cross-correlation patterns in social opinion formation with sequential data

Science.gov (United States)

Chakrabarti, Anindya S.

2016-11-01

Recent research on large-scale internet data suggests existence of patterns in the collective behavior of billions of people even though each of them may pursue own activities. In this paper, we interpret online rating activity as a process of forming social opinion about individual items, where people sequentially choose a rating based on the current information set comprising all previous ratings and own preferences. We construct an opinion index from the sequence of ratings and we show that (1) movie-specific opinion converges much slower than an independent and identically distributed (i.i.d.) sequence of ratings, (2) rating sequence for individual movies shows lesser variation compared to an i.i.d. sequence of ratings, (3) the probability density function of the asymptotic opinions has more spread than that defined over opinion arising from i.i.d. sequence of ratings, (4) opinion sequences across movies are correlated with significantly higher and lower correlation compared to opinion constructed from i.i.d. sequence of ratings, creating a bimodal cross-correlation structure. By decomposing the temporal correlation structures from panel data of movie ratings, we show that the social effects are very prominent whereas group effects cannot be differentiated from those of surrogate data and individual effects are quite small. The former explains a large part of extreme positive or negative correlations between sequences of opinions. In general, this method can be applied to any rating data to extract social or group-specific effects in correlation structures. We conclude that in this particular case, social effects are important in opinion formation process.

Correlation and path analysis of grain yield and morphological traits in test–cross populations of maize

NARCIS (Netherlands)

Sreckov, Z.; Nastasic, A.; Bocanski, J.; Djalovic, I.; Vukosavljev, M.; Jockovic, B.

2011-01-01

One of the goals of this paper was to determine correlation between grain yield, like the most important agronomic trait, and traits of the plant and ear that are influencing on the grain yield, in two test-cross populations, which are formed by crossing progenies of NSU(1) population after 17

Two dimension MDW OCDMA code cross-correlation for reduction of phase induced intensity noise

Directory of Open Access Journals (Sweden)

Sh. Ahmed Israa

2017-01-01

Full Text Available In this paper, we first review 2-D MDW code cross correlation equations and table to be improved significantly by using code correlation properties. These codes can be used in the synchronous optical CDMA systems for multi access interference cancellation and maximum suppress the phase induced intensity noise. Low Psr is due to the reduction of interference noise that is induced by the 2-D MDW code PIIN suppression. High data rate causes increases in BER, requires high effective power and severely deteriorates the system performance. The 2-D W/T MDW code has an excellent system performance where the value of PIIN is suppressed as low as possible at the optimum Psr with high data bit rate. The 2-D MDW code shows better tolerance to PIIN in comparison to others with enhanced system performance. We prove by numerical analysis that the PIIN maximally suppressed by MDW code through the minimizing property of cross correlation in comparison to 2-D PDC and 2-D MQC OCDMA code.scheme systems.

Two dimension MDW OCDMA code cross-correlation for reduction of phase induced intensity noise

Science.gov (United States)

Ahmed, Israa Sh.; Aljunid, Syed A.; Nordin, Junita M.; Dulaimi, Layth A. Khalil Al; Matem, Rima

2017-11-01

In this paper, we first review 2-D MDW code cross correlation equations and table to be improved significantly by using code correlation properties. These codes can be used in the synchronous optical CDMA systems for multi access interference cancellation and maximum suppress the phase induced intensity noise. Low Psr is due to the reduction of interference noise that is induced by the 2-D MDW code PIIN suppression. High data rate causes increases in BER, requires high effective power and severely deteriorates the system performance. The 2-D W/T MDW code has an excellent system performance where the value of PIIN is suppressed as low as possible at the optimum Psr with high data bit rate. The 2-D MDW code shows better tolerance to PIIN in comparison to others with enhanced system performance. We prove by numerical analysis that the PIIN maximally suppressed by MDW code through the minimizing property of cross correlation in comparison to 2-D PDC and 2-D MQC OCDMA code.scheme systems.

Detectability of the 21-cm CMB cross-correlation from the epoch of reionization

NARCIS (Netherlands)

Tashiro, Hiroyuki; Aghanim, Nabila; Langer, Mathieu; Douspis, Marian; Zaroubi, Saleem; Jelic, Vibor

The 21-cm line fluctuations and the cosmic microwave background (CMB) are powerful probes of the epoch of reionization of the Universe. We study the potential of the cross-correlation between 21-cm line fluctuations and CMB anisotropy to obtain further constraints on the reionization history. We

Evidence for gravitational lensing of the cosmic microwave background polarization from cross-correlation with the cosmic infrared background.

Science.gov (United States)

Ade, P A R; Akiba, Y; Anthony, A E; Arnold, K; Atlas, M; Barron, D; Boettger, D; Borrill, J; Borys, C; Chapman, S; Chinone, Y; Dobbs, M; Elleflot, T; Errard, J; Fabbian, G; Feng, C; Flanigan, D; Gilbert, A; Grainger, W; Halverson, N W; Hasegawa, M; Hattori, K; Hazumi, M; Holzapfel, W L; Hori, Y; Howard, J; Hyland, P; Inoue, Y; Jaehnig, G C; Jaffe, A; Keating, B; Kermish, Z; Keskitalo, R; Kisner, T; Le Jeune, M; Lee, A T; Leitch, E M; Linder, E; Lungu, M; Matsuda, F; Matsumura, T; Meng, X; Miller, N J; Morii, H; Moyerman, S; Myers, M J; Navaroli, M; Nishino, H; Paar, H; Peloton, J; Poletti, D; Quealy, E; Rebeiz, G; Reichardt, C L; Richards, P L; Ross, C; Rotermund, K; Schanning, I; Schenck, D E; Sherwin, B D; Shimizu, A; Shimmin, C; Shimon, M; Siritanasak, P; Smecher, G; Spieler, H; Stebor, N; Steinbach, B; Stompor, R; Suzuki, A; Takakura, S; Tikhomirov, A; Tomaru, T; Wilson, B; Yadav, A; Zahn, O

2014-04-04

We reconstruct the gravitational lensing convergence signal from cosmic microwave background (CMB) polarization data taken by the Polarbear experiment and cross-correlate it with cosmic infrared background maps from the Herschel satellite. From the cross spectra, we obtain evidence for gravitational lensing of the CMB polarization at a statistical significance of 4.0σ and indication of the presence of a lensing B-mode signal at a significance of 2.3σ. We demonstrate that our results are not biased by instrumental and astrophysical systematic errors by performing null tests, checks with simulated and real data, and analytical calculations. This measurement of polarization lensing, made via the robust cross-correlation channel, not only reinforces POLARBEAR auto-correlation measurements, but also represents one of the early steps towards establishing CMB polarization lensing as a powerful new probe of cosmology and astrophysics.

Bias of damped Lyman-α systems from their cross-correlation with CMB lensing

Science.gov (United States)

Alonso, D.; Colosimo, J.; Font-Ribera, A.; Slosar, A.

2018-04-01

We cross-correlate the positions of damped Lyman-α systems (DLAs) and their parent quasar catalog with a convergence map derived from the Planck cosmic microwave background (CMB) temperature data. We make consistent measurements of the lensing signal of both samples in both Fourier and configuration space. By interpreting the excess signal present in the DLA catalog with respect to the parent quasar catalog as caused by the large scale structure traced by DLAs, we are able to infer the bias of these objects: bDLA=2.6±0.9. These results are consistent with previous measurements made in cross-correlation with the Lyman-α forest, although the current noise in the lensing data and the low number density of DLAs limits the constraining power of this measurement. We discuss the robustness of the analysis with respect to a number different systematic effects and forecast prospects of carrying out this measurement with data from future experiments.

Measuring time-of-flight in an ultrasonic LPS system using generalized cross-correlation.

Science.gov (United States)

Villladangos, José Manuel; Ureña, Jesús; García, Juan Jesús; Mazo, Manuel; Hernández, Alvaro; Jiménez, Ana; Ruíz, Daniel; De Marziani, Carlos

2011-01-01

In this article, a time-of-flight detection technique in the frequency domain is described for an ultrasonic local positioning system (LPS) based on encoded beacons. Beacon transmissions have been synchronized and become simultaneous by means of the DS-CDMA (direct-sequence code Division multiple access) technique. Every beacon has been associated to a 255-bit Kasami code. The detection of signal arrival instant at the receiver, from which the distance to each beacon can be obtained, is based on the application of the generalized cross-correlation (GCC), by using the cross-spectral density between the received signal and the sequence to be detected. Prior filtering to enhance the frequency components around the carrier frequency (40 kHz) has improved estimations when obtaining the correlation function maximum, which implies an improvement in distance measurement precision. Positioning has been achieved by using hyperbolic trilateration, based on the time differences of arrival (TDOA) between a reference beacon and the others.

X-ray fluorescence investigation of gilded and enamelled silver: The case study of four medieval processional crosses from central Italy

Science.gov (United States)

Ferretti, Marco; Polese, Claudia; Roldán García, Clodoaldo

2013-05-01

The presence of multilayered structures is common in such cultural artefacts as paintings, corroded metals, objects that underwent a whatever form of surface qualification. One of the most usual and complete ways to investigate such structures is observing a cross section, which requires sampling. There are however situations where at least part of the stratigraphic information can be derived non-destructively: the literature shows that X-ray fluorescence (XRF) has frequently been used, in recent years, for this purpose, with special regard to paintings and gilded metals. Aim of this paper is to further explore the suitability of XRF-based techniques to characterise multilayered structures. This is achieved by introducing improvements, with respect to previous works, in both equipment and data processing. The method, that has been developed for gilded and enamelled silver artefacts, relies on optimum excitation conditions provided for silver and on the relationship existing between the ratio AgKα/AgKβ of its fluorescence lines and the gilding thickness itself. The coating (gilding or enamel) thickness is derived by verifying the condition CKα,Ag = CKβ,Ag, where CKα,Ag and CKβ,Ag are the mass fractions of silver calculated on the lines AgKα and AgKβ, respectively. The calculations are carried out by PyMCA, a Fundamental Parameters code that implements the analysis of multilayered samples. As a case study we investigated in situ the four processional crosses of Borbona, Sant'Elpidio, Rosciolo and Forcella, made of a wood core with attached gilt and embossed silver sheets and enamelled silver plates. The analyses allowed to distinguish ancient restorations from original parts, to characterise the enamels and find their composition consistent with the dates of manufacturing and, as regards the cross of Rosciolo, to hypothesize the contribution of different "hands" in its manufacturing.

Semi-Supervised Multi-View Ensemble Learning Based On Extracting Cross-View Correlation

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ZALL, R.

2016-05-01

Full Text Available Correlated information between different views incorporate useful for learning in multi view data. Canonical correlation analysis (CCA plays important role to extract these information. However, CCA only extracts the correlated information between paired data and cannot preserve correlated information between within-class samples. In this paper, we propose a two-view semi-supervised learning method called semi-supervised random correlation ensemble base on spectral clustering (SS_RCE. SS_RCE uses a multi-view method based on spectral clustering which takes advantage of discriminative information in multiple views to estimate labeling information of unlabeled samples. In order to enhance discriminative power of CCA features, we incorporate the labeling information of both unlabeled and labeled samples into CCA. Then, we use random correlation between within-class samples from cross view to extract diverse correlated features for training component classifiers. Furthermore, we extend a general model namely SSMV_RCE to construct ensemble method to tackle semi-supervised learning in the presence of multiple views. Finally, we compare the proposed methods with existing multi-view feature extraction methods using multi-view semi-supervised ensembles. Experimental results on various multi-view data sets are presented to demonstrate the effectiveness of the proposed methods.

Synthesis and Sensing Applications of Fluorescent 3-Cinnamoyl Coumarins

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Preeti Yadav

2015-12-01

Full Text Available We have synthesized two novel fluorescent 3-(4-diethylaminocinnamoyl coumarins that exhibit fluorescence quenching upon exposure to a nerve agent simulant, diethylchlorophosphate (DCP, providing a basis for rapid and sensitive DCP chemosensing. Furthermore, these coumarin derivatives display two-photon fluorescence upon illumination with near-infrared laser pulses and their two-photon (TP absorption cross-section was evaluated. The potential for TP bio-imaging of these compounds was investigated by their cellular uptake in HeLa cells by TP confocal microscopy.

Characterization of the photoreaction between DNA and aminomethyl-trimethylpsoralen using absorption and fluorescence spectroscopy

International Nuclear Information System (INIS)

Johnston, B.H.; Hearst, J.E.

1981-01-01

The use of absorption and fluorescence spectroscopy for following the progress of the photoreaction between DNA and 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) has been investigated. Absorption at long wavelengths and fluorescence both decline upon intercalation of AMT into the DNA helix. The loss of fluorescence from AMT and the accompanying appearance of monoadduct fluorescence upon irradiation by UV light can be easily followed by using the excitation beam of a spectrofluorometer as the source of irradiation and monitoring the changing emission spectrum. Where cross-link formation is possible, the subsequent decline of monoadduct fluorescence is seen as well. This suggests that the 4',5'-monoadduct is a precursor of cross-links. Both monoaddition and cross-linking are more rapid with poly d(A-T) than with calf thymus DNA or poly d(A.T). Excitation spectra can be helpful in resolving the levels of AMT and 4',5'-monoadduct when both are contributing to the emission spectrum. Some changes are observed in the emission spectrum of AMT-poly d(A.T) monoadducts after prolonged irradiation which indicate further photoreaction. (author)

An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy

International Nuclear Information System (INIS)

Dunsby, C; Lanigan, P M P; McGinty, J; Elson, D S; Requejo-Isidro, J; Munro, I; Galletly, N; McCann, F; Treanor, B; Oenfelt, B; Davis, D M; Neil, M A A; French, P M W

2004-01-01

Fluorescence imaging is used widely in microscopy and macroscopic imaging applications for fields ranging from biomedicine to materials science. A critical component for any fluorescence imaging system is the excitation source. Traditionally, wide-field systems use filtered thermal or arc-generated white light sources, while point scanning confocal microscope systems require spatially coherent (point-like) laser sources. Unfortunately, the limited range of visible wavelengths available from conventional laser sources constrains the design and usefulness of fluorescent probes in confocal microscopy. A 'hands-off' laser-like source, electronically tunable across the visible spectrum, would be invaluable for fluorescence imaging and provide new opportunities, e.g. automated excitation fingerprinting and in situ measurement of excitation cross-sections. Yet more information can be obtained using fluorescence lifetime imaging (FLIM), which requires that the light source be pulsed or rapidly modulated. We show how a white light continuum, generated by injecting femtosecond optical radiation into a micro-structured optical fibre, coupled with a simple prism-based tunable filter arrangement, can fulfil all these roles as a continuously electronically tunable (435-1150 nm) visible ultrafast light source in confocal, wide-field and FLIM systems

Efficacy of NGR peptide-modified PEGylated quantum dots for crossing the blood-brain barrier and targeted fluorescence imaging of glioma and tumor vasculature.

Science.gov (United States)

Huang, Ning; Cheng, Si; Zhang, Xiang; Tian, Qi; Pi, Jiangli; Tang, Jun; Huang, Qing; Wang, Feng; Chen, Jin; Xie, Zongyi; Xu, Zhongye; Chen, Weifu; Zheng, Huzhi; Cheng, Yuan

2017-01-01

Delivery of imaging agents to brain glioma is challenging because the blood-brain barrier (BBB) functions as a physiological checkpoint guarding the central nervous system from circulating large molecules. Moreover, the ability of existing probes to target glioma has been insufficient and needs to be improved. In present study, PEG-based long circulation, CdSe/ZnS quantum dots (QDs)-based nanoscale and fluorescence, asparagines-glycine-arginine peptides (NGR)-based specific CD13 recognition were integrated to design and synthesize a novel nanoprobe by conjugating biotinylated NGR peptides to avidin-PEG-coated QDs. Our data showed that the NGR-PEG-QDs were nanoscale with less than 100 nm and were stable in various pH (4.0~8.0). These nanomaterials with non-toxic concentrations could cross the BBB and target CD13-overexpressing glioma and tumor vasculature in vitro and in vivo, contributing to fluorescence imaging of this brain malignancy. These achievements allowed groundbreaking technological advances in targeted fluorescence imaging for the diagnosis and surgical removal of glioma, facilitating potential transformation toward clinical nanomedicine. Copyright © 2016 Elsevier Inc. All rights reserved.

Laser-Induced Fluorescence (LIF) from plant foliage

Science.gov (United States)

Chappelle, Emmett W.; Williams, Darrel L.

1987-01-01

The fluorescence spectra and fluorescence induction kinetics of green plants excited at 337 nm by a laser were studied. They correlate with plant type, as well as with changes in the physiology of the plant as the result of stress. The plant types studied include herbaceous dicots, monocots, hardwoods, conifers, and algae. These plant types could be identified on the basis of differences in either the number of fluorescent bands or the relative intensity of the bands. Differences in fluorescent spectra which could be related to vigor status are observed in conifers located in an area of high atmospheric deposition. Changes in the fluorescence spectra and induction kinetics are also seen in plants grown under conditions of nutrient deficiency and drought stress.

Retrieving the elastodynamic Green's function of an arbitrary inhomogeneous medium by cross correlation

International Nuclear Information System (INIS)

Wapenaar, Kees

2004-01-01

A correlation-type reciprocity theorem is used to show that the elastodynamic Green's function of any inhomogeneous medium (random or deterministic) can be retrieved from the cross correlation of two recordings of a wave field at different receiver locations at the free surface. Unlike in other derivations, which apply to diffuse wave fields in random media or irregular finite bodies, no assumptions are made about the diffusivity of the wave field. In a second version, it is assumed that the wave field is diffuse due to many uncorrelated sources inside the medium

Imaging endosomes and autophagosomes in whole mammalian cells using correlative cryo-fluorescence and cryo-soft X-ray microscopy (cryo-CLXM)

International Nuclear Information System (INIS)

Duke, Elizabeth M.H.; Razi, Minoo; Weston, Anne; Guttmann, Peter; Werner, Stephan; Henzler, Katja; Schneider, Gerd; Tooze, Sharon A.; Collinson, Lucy M.

2014-01-01

Cryo-soft X-ray tomography (cryo-SXT) is a powerful imaging technique that can extract ultrastructural information from whole, unstained mammalian cells as close to the living state as possible. Subcellular organelles including the nucleus, the Golgi apparatus and mitochondria have been identified by morphology alone, due to the similarity in contrast to transmission electron micrographs. In this study, we used cryo-SXT to image endosomes and autophagosomes, organelles that are particularly susceptible to chemical fixation artefacts during sample preparation for electron microscopy. We used two approaches to identify these compartments. For early and recycling endosomes, which are accessible to externally-loaded markers, we used an anti-transferrin receptor antibody conjugated to 10 nm gold particles. For autophagosomes, which are not accessible to externally-applied markers, we developed a correlative cryo-fluorescence and cryo-SXT workflow (cryo-CLXM) to localise GFP-LC3 and RFP-Atg9. We used a stand-alone cryo-fluorescence stage in the home laboratory to localise the cloned fluorophores, followed by cryo-soft X-ray tomography at the synchrotron to analyse cellular ultrastructure. We mapped the 3D ultrastructure of the endocytic and autophagic structures, and discovered clusters of omegasomes arising from ‘hotspots’ on the ER. Thus, immunogold markers and cryo-CLXM can be used to analyse cellular processes that are inaccessible using other imaging modalities. - Highlights: • We image whole, unstained mammalian cells using cryo-soft X-ray tomography. • Endosomes are identified using a gold marker for the transferrin receptor. • A new workflow for correlative cryo-fluorescence and cryo-SXT is used to locate early autophagosomes. • Interactions between endosomes, endoplasmic reticulum and forming autophagosomes are mapped in 3D. • Multiple omegasomes are shown to form at ‘hotspots’ on the endoplasmic reticulum

Connecting active to passive fluorescence with photosynthesis: a method for evaluating remote sensing measurements of Chl fluorescence.

Science.gov (United States)

Magney, Troy S; Frankenberg, Christian; Fisher, Joshua B; Sun, Ying; North, Gretchen B; Davis, Thomas S; Kornfeld, Ari; Siebke, Katharina

2017-09-01

Recent advances in the retrieval of Chl fluorescence from space using passive methods (solar-induced Chl fluorescence, SIF) promise improved mapping of plant photosynthesis globally. However, unresolved issues related to the spatial, spectral, and temporal dynamics of vegetation fluorescence complicate our ability to interpret SIF measurements. We developed an instrument to measure leaf-level gas exchange simultaneously with pulse-amplitude modulation (PAM) and spectrally resolved fluorescence over the same field of view - allowing us to investigate the relationships between active and passive fluorescence with photosynthesis. Strongly correlated, slope-dependent relationships were observed between measured spectra across all wavelengths (F λ , 670-850 nm) and PAM fluorescence parameters under a range of actinic light intensities (steady-state fluorescence yields, F t ) and saturation pulses (maximal fluorescence yields, F m ). Our results suggest that this method can accurately reproduce the full Chl emission spectra - capturing the spectral dynamics associated with changes in the yields of fluorescence, photochemical (ΦPSII), and nonphotochemical quenching (NPQ). We discuss how this method may establish a link between photosynthetic capacity and the mechanistic drivers of wavelength-specific fluorescence emission during changes in environmental conditions (light, temperature, humidity). Our emphasis is on future research directions linking spectral fluorescence to photosynthesis, ΦPSII, and NPQ. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Anthracene Fluorescence Quenching by a Tetrakis (Ketocarboxamide Cavitand

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Tibor Zoltan Janosi

2014-01-01

Full Text Available Quenching of both fluorescence lifetime and fluorescence intensity of anthracene was investigated in the presence of a newly derived tetrakis (ketocarboxamide cavitand at various concentrations. Time-correlated single photon counting method was applied for the lifetime measurements. A clear correlation between the fluorescence lifetime of anthracene as a function of cavitand concentration in dimethylformamide solution was observed. The bimolecular collisional quenching constant was derived from the decrease of lifetime. Fluorescence intensity was measured in the emission wavelength region around 400 nm as a result of excitation at 280 nm. Effective quenching was observed in the presence of the cavitand. The obtained Stern-Volmer plot displayed upward curvature. The results did not follow even extended Stern-Volmer behavior, often used to describe deviations from static bimolecular quenching. To explain our results we adopted the Smoluchowski model and obtained a reasonable estimate for the molecular radius of the cavitand in solution.

Remote detection of weak aftershocks of the DPRK underground explosions using waveform cross correlation

Science.gov (United States)

Le Bras, R.; Rozhkov, M.; Bobrov, D.; Kitov, I. O.; Sanina, I.

2017-12-01

Association of weak seismic signals generated by low-magnitude aftershocks of the DPRK underground tests into event hypotheses represent a challenge for routine automatic and interactive processing at the International Data Centre (IDC) of the Comprehensive Nuclear-Test-Ban Treaty Organization, due to the relatively low station density of the International Monitoring System (IMS) seismic network. Since 2011, as an alternative, the IDC has been testing various prototype techniques of signal detection and event creation based on waveform cross correlation. Using signals measured by seismic stations of the IMS from DPRK explosions as waveform templates, the IDC detected several small (estimated mb between 2.2 and 3.6) seismic events after two DPRK tests conducted on September 9, 2016 and September 3, 2017. The obtained detections were associated with reliable event hypothesis and then used to locate these events relative to the epicenters of the DPRK explosions. We observe high similarity of the detected signals with the corresponding waveform templates. The newly found signals also correlate well between themselves. In addition, the values of the signal-to-noise ratios (SNR) estimated using the traces of cross correlation coefficients, increase with template length (from 5 s to 150 s), providing strong evidence in favour of their spatial closeness, which allows interpreting them as explosion aftershocks. We estimated the relative magnitudes of all aftershocks using the ratio of RMS amplitudes of the master and slave signal in the cross correlation windows characterized by the highest SNR. Additional waveform data from regional non-IMS stations MDJ and SEHB provide independent validation of these aftershock hypotheses. Since waveform templates from any single master event may be sub-efficient at some stations, we have also developed a method of joint usage of the DPRK and the biggest aftershocks templates to build more robust event hypotheses.

Endoanal MRI of the anal sphincter complex: correlation with cross-sectional anatomy and histology

NARCIS (Netherlands)

S.M. Hussain (Shahid); J. Stoker (Jacob); A.W. Zwamborn; J.C. den Hollander (Jan); J.-W. Kuiper (Jan-Willem); C.A. Entius; J.S. Lameris

1996-01-01

textabstractThe purpose of this study was to correlate the in vivo endoanal MRI findings of the anal sphincter with the cross-sectional anatomy and histology. Fourteen patients with rectal tumours were examined with a rigid endoanal MR coil before undergoing

Towards sensitive, high-throughput, biomolecular assays based on fluorescence lifetime

Science.gov (United States)

Ioanna Skilitsi, Anastasia; Turko, Timothé; Cianfarani, Damien; Barre, Sophie; Uhring, Wilfried; Hassiepen, Ulrich; Léonard, Jérémie

2017-09-01

Time-resolved fluorescence detection for robust sensing of biomolecular interactions is developed by implementing time-correlated single photon counting in high-throughput conditions. Droplet microfluidics is used as a promising platform for the very fast handling of low-volume samples. We illustrate the potential of this very sensitive and cost-effective technology in the context of an enzymatic activity assay based on fluorescently-labeled biomolecules. Fluorescence lifetime detection by time-correlated single photon counting is shown to enable reliable discrimination between positive and negative control samples at a throughput as high as several hundred samples per second.

Erbium-based optical coherent transient correlator for the 1.5-micron communication bands

Science.gov (United States)

Harris, Todd Louis

2001-08-01

Correlators are needed in communications, memory, and signal processing applications to perform cross- correlations for tasks such as address-header decoding for data-packet switching, spread spectrum and code division multiple access communication, associative memory, database searching, and pattern recognition. Correlators based on optical coherent transients, Fourier theory, and holography can potentially perform real-time correlations with multi-phase encoded information at gigahertz bandwidths, a capability conventional electronics lack. The first operation of spatial-spectral holographic correlators in the 1.5-gm communication bands was demonstrated at 1536 nm using Er3+:Y 2SiO5 and the correlator processed multi-phase encoded optical pulses. Real-time decoding of 20-bit binary-phase-shift key encoded address-header pulses is demonstrated using stimulated photon echoes in a phase-matched crossed-beam configuration; this function is required for coherent transient optical data routing and packet switching. Optical 30-symbol quadriphase-shift keyed (QPSK) and binary-phase-shift keyed (BPSK) codes were processed, and the results demonstrated the ability of such correlators to process QPSK codes and BPSK codes with the same apparatus. The high-fidelity correlations exhibit the low sidelobe characteristics expected for the codes used. The 4I15/2 and 4I13/2 crystal field levels of 0.005% Er3+:Y2O3 were measured by absorption and laser excited fluorescence on oriented samples. Site selective fluorescence distinguished transitions of Er3+ in crystallographic sites of C2 and C3i symmetry. The paramagnetic g-tensors for ions in sites of C2 symmetry were measured by orientation dependent Zeeman absorption spectroscopy. For the lowest crystal field level of 4I15/2 the g-tensor principal x-axis in the (100) plane is tipped +2.06° from [001] and principal g-values are: gz = 11.93, gx = 1.603, and gy = 4.711. For the lowest crystal field level of 4I13/2 the g

Phenotyping of Arabidopsis Drought Stress Response Using Kinetic Chlorophyll Fluorescence and Multicolor Fluorescence Imaging

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Jieni Yao

2018-05-01

Full Text Available Plant responses to drought stress are complex due to various mechanisms of drought avoidance and tolerance to maintain growth. Traditional plant phenotyping methods are labor-intensive, time-consuming, and subjective. Plant phenotyping by integrating kinetic chlorophyll fluorescence with multicolor fluorescence imaging can acquire plant morphological, physiological, and pathological traits related to photosynthesis as well as its secondary metabolites, which will provide a new means to promote the progress of breeding for drought tolerant accessions and gain economic benefit for global agriculture production. Combination of kinetic chlorophyll fluorescence and multicolor fluorescence imaging proved to be efficient for the early detection of drought stress responses in the Arabidopsis ecotype Col-0 and one of its most affected mutants called reduced hyperosmolality-induced [Ca2+]i increase 1. Kinetic chlorophyll fluorescence curves were useful for understanding the drought tolerance mechanism of Arabidopsis. Conventional fluorescence parameters provided qualitative information related to drought stress responses in different genotypes, and the corresponding images showed spatial heterogeneities of drought stress responses within the leaf and the canopy levels. Fluorescence parameters selected by sequential forward selection presented high correlations with physiological traits but not morphological traits. The optimal fluorescence traits combined with the support vector machine resulted in good classification accuracies of 93.3 and 99.1% for classifying the control plants from the drought-stressed ones with 3 and 7 days treatments, respectively. The results demonstrated that the combination of kinetic chlorophyll fluorescence and multicolor fluorescence imaging with the machine learning technique was capable of providing comprehensive information of drought stress effects on the photosynthesis and the secondary metabolisms. It is a promising

Kinematic cross-correlation induces sensory integration across separate objects.

Science.gov (United States)

Debats, Nienke B; Ernst, Marc O; Heuer, Herbert

2017-12-01

In a basic cursor-control task, the perceived positions of the hand and the cursor are biased towards each other. We recently found that this phenomenon conforms to the reliability-based weighting mechanism of optimal multisensory integration. This indicates that optimal integration is not restricted to sensory signals originating from a single source, as is the prevailing view, but that it also applies to separate objects that are connected by a kinematic relation (i.e. hand and cursor). In the current study, we examined which aspects of the kinematic relation are crucial for eliciting the sensory integration: (i) the cross-correlation between kinematic variables of the hand and cursor trajectories, and/or (ii) an internal model of the hand-cursor kinematic transformation. Participants made out-and-back movements from the centre of a semicircular workspace to its boundary, after which they judged the position where either their hand or the cursor hit the boundary. We analysed the position biases and found that the integration was strong in a condition with high kinematic correlations (a straight hand trajectory was mapped to a straight cursor trajectory), that it was significantly reduced for reduced kinematic correlations (a straight hand trajectory was transformed into a curved cursor trajectory) and that it was not affected by the inability to acquire an internal model of the kinematic transformation (i.e. by the trial-to-trial variability of the cursor curvature). These findings support the idea that correlations play a crucial role in multisensory integration irrespective of the number of sensory sources involved. © 2017 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.

Mapping vortex-like hydrodynamic flow in microfluidic networks using fluorescence correlation spectroscopy

Energy Technology Data Exchange (ETDEWEB)

Ke Liu; Yu Tian; Burrows, Sean M.; Reif, Randall D. [Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061 (United States); Pappas, Dimitri, E-mail: d.pappas@ttu.edu [Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061 (United States)

2009-09-28

The ability to quickly measure flow parameters in microfluidic devices is critical for micro total analysis system ({mu}TAS) applications. Macrofluidic methods to assess flow suffer from limitations that have made conventional methods unsuitable for the flow behavior profiling. Single molecule fluorescence correlation spectroscopy (FCS) has been employed in our study to characterize the fluidic vortex generating at a T-shape junction of microscale channels. Due to its high spatial and temporal resolution, the corresponding magnitudes relative to different flow rates in the main channel can be quantitatively differentiated using flow time ({tau}{sub F}) measurements of dye molecules traversing the detection volume in buffer solution. Despite the parabolic flow in the channel upstream, a heterogeneous distribution of flow has been detected across the channel intersection. In addition, our current observations also confirmed the aspect of vortex-shaped flow in low-shear design that was developed previously for cell culture. This approach not only overcomes many technical barriers for examining hydrodynamic vortices and movements in miniature structures without physically integrating any probes, but it is also especially useful for the hydrodynamic studies in polymer-glass based micro -reactor and -mixer.

The cross-correlation between 21 cm intensity mapping maps and the Lyα forest in the post-reionization era

Energy Technology Data Exchange (ETDEWEB)

Carucci, Isabella P. [SISSA—International School for Advanced Studies, Via Bonomea 265, 34136 Trieste (Italy); Villaescusa-Navarro, Francisco [Center for Computational Astrophysics, 160 5th Avenue, New York, NY, 10010 (United States); Viel, Matteo, E-mail: ipcarucci@sissa.it, E-mail: fvillaescusa@simonsfoundation.org, E-mail: viel@oats.inaf.it [INAF—Osservatorio Astronomico di Trieste, Via Tiepolo 11, 34143, Trieste (Italy)

2017-04-01

We investigate the cross-correlation signal between 21cm intensity mapping maps and the Lyα forest in the fully non-linear regime using state-of-the-art hydrodynamic simulations. The cross-correlation signal between the Lyα forest and 21cm maps can provide a coherent and comprehensive picture of the neutral hydrogen (HI) content of our Universe in the post-reionization era, probing both its mass content and volume distribution. We compute the auto-power spectra of both fields together with their cross-power spectrum at z = 2.4 and find that on large scales the fields are completely anti-correlated. This anti-correlation arises because regions with high (low) 21cm emission, such as those with a large (low) concentration of damped Lyα systems, will show up as regions with low (high) transmitted flux. We find that on scales smaller than k ≅ 0.2 h Mpc{sup −1} the cross-correlation coefficient departs from −1, at a scale where non-linearities show up. We use the anisotropy of the power spectra in redshift-space to determine the values of the bias and of the redshift-space distortion parameters of both fields. We find that the errors on the value of the cosmological and astrophysical parameters could decrease by 30% when adding data from the cross-power spectrum, in a conservative analysis. Our results point out that linear theory is capable of reproducing the shape and amplitude of the cross-power up to rather non-linear scales. Finally, we find that the 21cm-Lyα cross-power spectrum can be detected by combining data from a BOSS-like survey together with 21cm intensity mapping observations by SKA1-MID with a S/N ratio higher than 3 in k element of [0.06,1] h Mpc{sup −1}. We emphasize that while the shape and amplitude of the 21cm auto-power spectrum can be severely affected by residual foreground contamination, cross-power spectra will be less sensitive to that and therefore can be used to identify systematics in the 21cm maps.

Cross correlation measurement of low frequency conductivity noise

Science.gov (United States)

Jain, Aditya Kumar; Nigudkar, Himanshu; Chakraborti, Himadri; Udupa, Aditi; Gupta, Kantimay Das

2018-04-01

In order to study the low frequency noise(1/f noise)an experimental technique based on cross correlation of two channels is presented. In this method the device under test (DUT)is connected to the two independently powered preamplifiers in parallel. The amplified signals from the two preamplifiers are fed to two channels of a digitizer. Subsequent data processing largelyeliminates the uncorrelated noise of the two channels. This method is tested for various commercial carbon/metal film resistors by measuring equilibrium thermal noise (4kBTR). The method is then modified to study the non-equilibrium low frequency noise of heterostructure samples using fiveprobe configuration. Five contact probes allow two parts of the sample to become two arms of a balanced bridge. This configuration helps in suppressing the effect of power supply fluctuations, bath temperature fluctuations and contact resistances.

Liquid-metal pin-fin pressure drop by correlation in cross flow

International Nuclear Information System (INIS)

Wang, Zhibi; Kuzay, T.M.; Assoufid, L.

1994-01-01

The pin-fin configuration is widely used as a heat transfer enhancement method in high-heat-flux applications. Recently, the pin-fin design with liquid-metal coolant was also applied to synchrotron-radiation beamline devices. This paper investigates the pressure drop in a pin-post design beamline mirror with liquid gallium as the coolant. Because the pin-post configuration is a relatively new concept, information in literature about pin-post mirrors or crystals is rare, and information about the pressure drop in pin-post mirrors with liquid metal as the coolant is even more sparse. Due to this the authors considered the cross flow in cylinder-array geometry, which is very similar to that of the pin-post, to examine the pressure drop correlation with liquid metals over pin fins. The cross flow of fluid with various fluid characteristics or properties through a tube bank was studied so that the results can be scaled to the pin-fin geometry with liquid metal as the coolant. Study lead to two major variables to influence the pressure drop: fluid properties, viscosity and density, and the relative length of the posts. Correlation of the pressure drop between long and short posts and the prediction of the pressure drop of liquid metal in the pin-post mirror and comparison with an existing experiment are addressed

The correlations between natural elements (K, U, Th) concentrations and thermal neutron absorption cross-section value (Σa) for rock samples of Carpatia area

International Nuclear Information System (INIS)

Swakon, J.; Cywicka-Jakiel, T.; Drozdowicz, E.; Gabanska, B.; Loskiewicz, J.; Woznicka, U.

1991-01-01

The paper presents a study of correlations between concentrations of potassium, uranium and thorium and thermal neutron absorption cross section in rock samples. This knowledge of correlation should help in recognizing the expansion ways and accumulation places of the elements responsible of high thermal neutron absorption cross section in some geological environments. The correlations show the existence of connections between the thermal neutron absorption cross section value and natural radioactivity elements concentration in rocks. The results confirm the existence of correlations between natural radioactive elements concentrations (particularly thorium) and thermal neutron absorption cross - section value in some rocks. (author). 12 refs, 23 figs, 6 tabs

Detection and identification of Cu2+ and Hg2+ based on the cross-reactive fluorescence responses of a dansyl-functionalized film in different solvents.

Science.gov (United States)

Cao, Yuan; Ding, Liping; Wang, Shihuai; Liu, Yuan; Fan, Junmei; Hu, Wenting; Liu, Ping; Fang, Yu

2014-01-08

A dansyl-functionalized fluorescent film sensor was specially designed and prepared by assembling dansyl on a glass plate surface via a long flexible spacer containing oligo(oxyethylene) and amine units. The chemical attachment of dansyl moieties on the surface was verified by contact angle, XPS, and fluorescence measurements. Solvent effect examination revealed that the polarity-sensitivity was retained for the surface-confined dansyl moieties. Fluorescence quenching studies in water declared that the dansyl-functionalized SAM possesses a higher sensitivity towards Hg(2+) and Cu(2+) than the other tested divalent metal ions including Zn(2+), Cd(2+), Co(2+), and Pb(2+). Further measurements of the fluorescence responses of the film towards Cu(2+) and Hg(2+) in three solvents including water, acetonitrile, and THF evidenced that the present film exhibits cross-reactive responses to these two metal ions. The combined signals from the three solvents provide a recognition pattern for both metal ions at a certain concentration and realize the identification between Hg(2+) and Cu(2+). Moreover, using principle component analysis, this method can be extended to identify metal ions that are hard to detect by the film sensor in water such as Co(2+) and Ni(2+).

Liposome encapsulation of fluorescent nanoparticles: Quantum dots and silica nanoparticles

International Nuclear Information System (INIS)

Chen, C.-S.; Yao Jie; Durst, Richard A.

2006-01-01

Quantum dots (QDs) and silica nanoparticles (SNs) are relatively new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs in liposomes and separated nanoparticle-loaded liposomes from unencapsulated nanoparticles by size exclusion chromatography. Fluorescence correlation spectroscopy was used to measure the average number of nanoparticles inside each liposome. Results indicated that nanoparticle-loaded liposomes were formed and separated from unencapsulated nanoparticles by using a Sepharose gel. As expected, fluorescence self-quenching of nanoparticles inside liposomes was not observed. Each liposome encapsulated an average of three QDs. These studies demonstrated that nanoparticles could be successfully encapsulated into liposomes and provided a methodology to quantify the number of nanoparticles inside each liposome by fluorescence correlation spectroscopy

An orange fluorescent protein tagging system for real-time pollen tracking.

Science.gov (United States)

Rice, J Hollis; Millwood, Reginald J; Mundell, Richard E; Chambers, Orlando D; Abercrombie, Laura L; Davies, H Maelor; Stewart, C Neal

2013-09-27

Monitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (PMPs) in open field production. A Nicotiana hybrid (Nicotiana. tabacum × Nicotiana glauca) shows limited male fertility and could be used as a bioconfined PMP platform. Effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification. We report the generation of a pollen tagging system utilizing an orange fluorescent protein to monitor pollen flow and as a visual assessment of transgene zygosity of the parent plant. This system was created to generate a tagged Nicotiana hybrid that could be used for the incidence of gene flow. Nicotiana tabacum 'TN 90' and Nicotiana glauca were successfully transformed via Agrobacterium tumefaciens to express the orange fluorescent protein gene, tdTomato-ER, in pollen and a green fluorescent protein gene, mgfp5-er, was expressed in vegetative structures of the plant. Hybrids were created that utilized the fluorescent proteins as a research tool for monitoring pollen movement and gene flow. Manual greenhouse crosses were used to assess hybrid sexual compatibility with N. tabacum, resulting in seed formation from hybrid pollination in 2% of crosses, which yielded non-viable seed. Pollen transfer to the hybrid formed seed in 19% of crosses and 10 out of 12 viable progeny showed GFP expression. The orange fluorescent protein is visible when expressed in the pollen of N. glauca, N. tabacum, and the Nicotiana hybrid, although hybrid pollen did not appear as bright as the parent lines. The hybrid plants, which show limited ability to outcross, could provide bioconfinement with the benefit of detectable pollen using this system. Fluorescent protein-tagging could be a valuable tool for breeding and in vivo ecological monitoring.

Evaluation of the performance of an ultrasonic cross-correlation flowmeter

International Nuclear Information System (INIS)

Bazerghi, H.; Serdula, K.J.

1977-09-01

An ultrasonic cross-correlation flowmeter, developed to assist in improving performance of heavy water plants, was evaluated. Overall performance of the flowmeter is satisfactory. The flowmeter is ideally suited to industrial applications and has an accuracy and repeatability comparable to many laboratory instruments. An accuracy of 3% is readily obtainable. This new 'clamp-on' portable flowmeter should prove useful in applications which provide flow measurements in systems where pipe penetration is too costly or not practical, verify or replace existing flowmeters, and measure flows in lines not previously instrumented to provide better control or to verify performance of systems

Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature.

Directory of Open Access Journals (Sweden)

Andrew Advani

Full Text Available Rarefaction of the renal microvasculature correlates with declining kidney function. However, current technologies commonly used for its evaluation are limited by their reliance on endothelial cell antigen expression and assessment in two dimensions. We set out to establish a widely applicable and unbiased optical sectioning method to enable three dimensional imaging and reconstruction of the renal microvessels based on their luminal filling. The kidneys of subtotally nephrectomized (SNx rats and their sham-operated counterparts were subjected to either routine two-dimensional immunohistochemistry or the novel technique of fluorescent microangiography (FMA. The latter was achieved by perfusion of the kidney with an agarose suspension of fluorescent polystyrene microspheres followed by optical sectioning of 200 µm thick cross-sections using a confocal microscope. The fluorescent microangiography method enabled the three-dimensional reconstruction of virtual microvascular casts and confirmed a reduction in both glomerular and peritubular capillary density in the kidneys of SNx rats, despite an overall increase in glomerular volume. FMA is an uncomplicated technique for evaluating the renal microvasculature that circumvents many of the limitations imposed by conventional analysis of two-dimensional tissue sections.

A cross-correlation objective function for least-squares migration and visco-acoustic imaging

KAUST Repository

Dutta, Gaurav

2014-08-05

Conventional acoustic least-squares migration inverts for a reflectivity image that best matches the amplitudes of the observed data. However, for field data applications, it is not easy to match the recorded amplitudes because of the visco-elastic nature of the earth and inaccuracies in the estimation of source signature and strength at different shot locations. To relax the requirement for strong amplitude matching of least-squares migration, we use a normalized cross-correlation objective function that is only sensitive to the similarity between the predicted and the observed data. Such a normalized cross-correlation objective function is also equivalent to a time-domain phase inversion method where the main emphasis is only on matching the phase of the data rather than the amplitude. Numerical tests on synthetic and field data show that such an objective function can be used as an alternative to visco-acoustic least-squares reverse time migration (Qp-LSRTM) when there is strong attenuation in the subsurface and the estimation of the attenuation parameter Qp is insufficiently accurate.

A cross-correlation objective function for least-squares migration and visco-acoustic imaging

KAUST Repository

Dutta, Gaurav; Sinha, Mrinal; Schuster, Gerard T.

2014-01-01

Conventional acoustic least-squares migration inverts for a reflectivity image that best matches the amplitudes of the observed data. However, for field data applications, it is not easy to match the recorded amplitudes because of the visco-elastic nature of the earth and inaccuracies in the estimation of source signature and strength at different shot locations. To relax the requirement for strong amplitude matching of least-squares migration, we use a normalized cross-correlation objective function that is only sensitive to the similarity between the predicted and the observed data. Such a normalized cross-correlation objective function is also equivalent to a time-domain phase inversion method where the main emphasis is only on matching the phase of the data rather than the amplitude. Numerical tests on synthetic and field data show that such an objective function can be used as an alternative to visco-acoustic least-squares reverse time migration (Qp-LSRTM) when there is strong attenuation in the subsurface and the estimation of the attenuation parameter Qp is insufficiently accurate.

Improved fluorescent X-ray image intensifying screen

International Nuclear Information System (INIS)

Landeghem, W.K. van; Suys, A.R.

1981-01-01

An X-ray image intensifying screen is described, which includes at least one fluorescent layer comprising phosphor particles dispersed in a binder and on top of such layer a protective layer containing a crosslinked polymer mass obtained by an acid-catalyzed reaction of a polymer or mixture of polymers containing reactive hydrogen atoms and a cross-linking agent, the cross-linking agent being an organic compound containing a plurality of etherified N-methylol groups. Examples are given of appropriate polymers and cross-linking agents. (author)

Evidence of Cross-correlation between the CMB Lensing and the γ-Ray Sky

Science.gov (United States)

Fornengo, Nicolao; Perotto, Laurence; Regis, Marco; Camera, Stefano

2015-03-01

We report the measurement of the angular power spectrum of cross-correlation between the unresolved component of the Fermi-LAT γ-ray sky maps and the cosmic microwave background lensing potential map reconstructed by the Planck satellite. The matter distribution in the universe determines the bending of light coming from the last scattering surface. At the same time, the matter density drives the growth history of astrophysical objects, including their capability at generating non-thermal phenomena, which in turn give rise to γ-ray emissions. The Planck lensing map provides information on the integrated distribution of matter, while the integrated history of γ-ray emitters is imprinted in the Fermi-LAT sky maps. We report here the first evidence of their correlation. We find that the multipole dependence of the cross-correlation measurement is in agreement with current models of the γ-ray luminosity function for active galactic nuclei and star-forming galaxies, with a statistical evidence of 3.0σ. Moreover, its amplitude can in general be matched only assuming that these extragalactic emitters are also the bulk contribution of the measured isotopic γ-ray background (IGRB) intensity. This leaves little room for a big contribution from galactic sources to the IGRB measured by Fermi-LAT, pointing toward direct evidence of the extragalactic origin of the IGRB.

EVIDENCE OF CROSS-CORRELATION BETWEEN THE CMB LENSING AND THE γ-RAY SKY

Energy Technology Data Exchange (ETDEWEB)

Fornengo, Nicolao; Regis, Marco [Dipartimento di Fisica, Università di Torino, I-10125 Torino (Italy); Perotto, Laurence [LPSC, Université Grenoble-Alpes, CNRS/IN2P3, 53, rue des Martyrs, F-38026 Grenoble Cedex (France); Camera, Stefano, E-mail: regis@to.infn.it [Jodrell Bank Centre for Astrophysics, The University of Manchester, Manchester M13 9PL (United Kingdom)

2015-03-01

We report the measurement of the angular power spectrum of cross-correlation between the unresolved component of the Fermi-LAT γ-ray sky maps and the cosmic microwave background lensing potential map reconstructed by the Planck satellite. The matter distribution in the universe determines the bending of light coming from the last scattering surface. At the same time, the matter density drives the growth history of astrophysical objects, including their capability at generating non-thermal phenomena, which in turn give rise to γ-ray emissions. The Planck lensing map provides information on the integrated distribution of matter, while the integrated history of γ-ray emitters is imprinted in the Fermi-LAT sky maps. We report here the first evidence of their correlation. We find that the multipole dependence of the cross-correlation measurement is in agreement with current models of the γ-ray luminosity function for active galactic nuclei and star-forming galaxies, with a statistical evidence of 3.0σ. Moreover, its amplitude can in general be matched only assuming that these extragalactic emitters are also the bulk contribution of the measured isotopic γ-ray background (IGRB) intensity. This leaves little room for a big contribution from galactic sources to the IGRB measured by Fermi-LAT, pointing toward direct evidence of the extragalactic origin of the IGRB.

Quadrature Errors and DC Offsets Calibration of Analog Complex Cross-Correlator for Interferometric Passive Millimeter-Wave Imaging Applications

Directory of Open Access Journals (Sweden)

Chao Wang

2018-02-01

Full Text Available The design and calibration of the cross-correlator are crucial issues for interferometric imaging systems. In this paper, an analog complex cross-correlator with output DC offsets and amplitudes calibration capability is proposed for interferometric passive millimeter-wave security sensing applications. By employing digital potentiometers in the low frequency amplification circuits of the correlator, the outputs characteristics of the correlator could be digitally controlled. A measurement system and a corresponding calibration scheme were developed in order to eliminate the output DC offsets and the quadrature amplitude error between the in-phase and the quadrature correlating subunits of the complex correlator. By using vector modulators to provide phase controllable correlated noise signals, the measurement system was capable of obtaining the output correlation circle of the correlator. When injected with −18 dBm correlated noise signals, the calibrated quadrature amplitude error was 0.041 dB and the calibrated DC offsets were under 26 mV, which was only 7.1% of the uncalibrated value. Furthermore, we also described a quadrature errors calibration algorithm in order to estimate the quadrature phase error and in order to improve the output phase accuracy of the correlator. After applying this calibration, we were able to reduce the output phase error of the correlator to 0.3°.

X-ray fluorescence investigation of gilded and enamelled silver: The case study of four medieval processional crosses from central Italy

International Nuclear Information System (INIS)

Ferretti, Marco; Polese, Claudia; Roldán García, Clodoaldo

2013-01-01

The presence of multilayered structures is common in such cultural artefacts as paintings, corroded metals, objects that underwent a whatever form of surface qualification. One of the most usual and complete ways to investigate such structures is observing a cross section, which requires sampling. There are however situations where at least part of the stratigraphic information can be derived non-destructively: the literature shows that X-ray fluorescence (XRF) has frequently been used, in recent years, for this purpose, with special regard to paintings and gilded metals. Aim of this paper is to further explore the suitability of XRF-based techniques to characterise multilayered structures. This is achieved by introducing improvements, with respect to previous works, in both equipment and data processing. The method, that has been developed for gilded and enamelled silver artefacts, relies on optimum excitation conditions provided for silver and on the relationship existing between the ratio AgKα/AgKβ of its fluorescence lines and the gilding thickness itself. The coating (gilding or enamel) thickness is derived by verifying the condition C Kα,Ag = C Kβ,Ag , where C Kα,Ag and C Kβ,Ag are the mass fractions of silver calculated on the lines AgKα and AgKβ, respectively. The calculations are carried out by PyMCA, a Fundamental Parameters code that implements the analysis of multilayered samples. As a case study we investigated in situ the four processional crosses of Borbona, Sant'Elpidio, Rosciolo and Forcella, made of a wood core with attached gilt and embossed silver sheets and enamelled silver plates. The analyses allowed to distinguish ancient restorations from original parts, to characterise the enamels and find their composition consistent with the dates of manufacturing and, as regards the cross of Rosciolo, to hypothesize the contribution of different “hands” in its manufacturing. - Highlights: ► We discuss a non-destructive XRF method for the

X-ray fluorescence investigation of gilded and enamelled silver: The case study of four medieval processional crosses from central Italy

Energy Technology Data Exchange (ETDEWEB)

Ferretti, Marco, E-mail: marco.ferretti@itabc.cnr.it [CNR, Istituto per le Tecnologie Applicate ai Beni Culturali, AdR RM1, Via Salaria km 29.300, 00016 Montelibretti (Roma) (Italy); Polese, Claudia, E-mail: claudia.polese87@hotmail.it [Dip. di Chimica, Università di Roma “Sapienza,” Piazzale Aldo Moro 5, 00185 Roma (Italy); Roldán García, Clodoaldo, E-mail: Clodoaldo.Roldan@uv.es [Instituto de Ciencia de Materiales, Parc Cientific Universidad de Valencia, C/Catedrático José Beltrán 2, 46980 Paterna (Valencia) (Spain)

2013-05-01

The presence of multilayered structures is common in such cultural artefacts as paintings, corroded metals, objects that underwent a whatever form of surface qualification. One of the most usual and complete ways to investigate such structures is observing a cross section, which requires sampling. There are however situations where at least part of the stratigraphic information can be derived non-destructively: the literature shows that X-ray fluorescence (XRF) has frequently been used, in recent years, for this purpose, with special regard to paintings and gilded metals. Aim of this paper is to further explore the suitability of XRF-based techniques to characterise multilayered structures. This is achieved by introducing improvements, with respect to previous works, in both equipment and data processing. The method, that has been developed for gilded and enamelled silver artefacts, relies on optimum excitation conditions provided for silver and on the relationship existing between the ratio AgKα/AgKβ of its fluorescence lines and the gilding thickness itself. The coating (gilding or enamel) thickness is derived by verifying the condition C{sub Kα,Ag} = C{sub Kβ,Ag}, where C{sub Kα,Ag} and C{sub Kβ,Ag} are the mass fractions of silver calculated on the lines AgKα and AgKβ, respectively. The calculations are carried out by PyMCA, a Fundamental Parameters code that implements the analysis of multilayered samples. As a case study we investigated in situ the four processional crosses of Borbona, Sant'Elpidio, Rosciolo and Forcella, made of a wood core with attached gilt and embossed silver sheets and enamelled silver plates. The analyses allowed to distinguish ancient restorations from original parts, to characterise the enamels and find their composition consistent with the dates of manufacturing and, as regards the cross of Rosciolo, to hypothesize the contribution of different “hands” in its manufacturing. - Highlights: ► We discuss a non

Foreground mitigation strategy for measuring the 21 cm-LAE cross-correlation

Science.gov (United States)

Yoshiura, Shintaro; Line, Jack L. B.; Kubota, Kenji; Hasegawa, Kenji; Takahashi, Keitaro

2018-05-01

The cross power spectrum of the 21 cm signal and Lyman-α emitters (LAEs) is a probe of the Epoch of Reionization. Astrophysical foregrounds do not correlate with the LAE distribution, though the foregrounds contribute to the error. To study the impact of foregrounds on the measurement, we assume realistic observation by the Murchison Widefield Array using a catalogue of radio galaxies, a LAE survey by the Subaru Hyper Supreme-Cam and the redshift of LAEs is determined by the Prime Focus Spectrograph. The HI distribution is estimated from a radiative transfer simulation with models based on results of radiation hydrodynamics simulation. Using these models, we found that the error of cross power spectrum is dominated by foreground terms. Furthermore, we estimate the effects of foreground removal, and find 99% of the foreground removal is required to detect the 21 cm-LAE signal at k ~ 0.4 h Mpc-1.

Endoanal MRI of the anal sphincter complex: correlation with cross-sectional anatomy and histology

NARCIS (Netherlands)

Hussain, S. M.; Stoker, J.; Zwamborn, A. W.; den Hollander, J. C.; Kuiper, J. W.; Entius, C. A.; Laméris, J. S.

1996-01-01

The purpose of this study was to correlate the in vivo endoanal MRI findings of the anal sphincter with the cross-sectional anatomy and histology. Fourteen patients with rectal tumours were examined with a rigid endoanal MR coil before undergoing abdominoperineal resection. In addition, 12 cadavers

Burst Detection and Localization using Discrete Wavelet Transform and Cross-Correlation

Directory of Open Access Journals (Sweden)

Eduardo Trutié-Carrero

2018-03-01

Full Text Available Burst in water distribution systems causes great loss of this natural resource, interrupts the water supply, damages the streets, builds and increases the transmission of infectious diseases. In this paper we propose a new algorithm that allows the detection and automatic localization of burst in water distribution systems. As for detection, the novelty is to use the wavelet correlation criterion to compute the statistical decision and compare it with a detection threshold. The novelty in the localization is to use the statistical operator cross-correlation. The algorithm was implemented in Octave and was validated with 32 signals acquired in the laboratory in a 26.7 m long steel pipe. In 16 signals burst were triggered which were detected under a false positive probability of 2 %. No false positives were present on the 16 signals where only noise was present.

Protein recognition by a pattern-generating fluorescent molecular probe

Science.gov (United States)

Pode, Zohar; Peri-Naor, Ronny; Georgeson, Joseph M.; Ilani, Tal; Kiss, Vladimir; Unger, Tamar; Markus, Barak; Barr, Haim M.; Motiei, Leila; Margulies, David

2017-12-01

Fluorescent molecular probes have become valuable tools in protein research; however, the current methods for using these probes are less suitable for analysing specific populations of proteins in their native environment. In this study, we address this gap by developing a unimolecular fluorescent probe that combines the properties of small-molecule-based probes and cross-reactive sensor arrays (the so-called chemical 'noses/tongues'). On the one hand, the probe can detect different proteins by generating unique identification (ID) patterns, akin to cross-reactive arrays. On the other hand, its unimolecular scaffold and selective binding enable this ID-generating probe to identify combinations of specific protein families within complex mixtures and to discriminate among isoforms in living cells, where macroscopic arrays cannot access. The ability to recycle the molecular device and use it to track several binding interactions simultaneously further demonstrates how this approach could expand the fluorescent toolbox currently used to detect and image proteins.

Decay of correlations between cross-polarized electromagnetic waves in a two-dimensional random medium.

Science.gov (United States)

Gorodnichev, E E

2018-04-01

The problem of multiple scattering of polarized light in a two-dimensional medium composed of fiberlike inhomogeneities is studied. The attenuation lengths for the density matrix elements are calculated. For a highly absorbing medium it is found that, as the sample thickness increases, the intensity of waves polarized along the fibers decays faster than the other density matrix elements. With further increase in the sample thickness, the off-diagonal elements which are responsible for correlations between the cross-polarized waves disappear. In the asymptotic limit of very thick samples the scattered light proves to be polarized perpendicular to the fibers. The difference in the attenuation lengths between the density matrix elements results in a nonmonotonic depth dependence of the degree of polarization. In the opposite case of a weakly absorbing medium, the off-diagonal element of the density matrix and, correspondingly, the correlations between the cross-polarized fields are shown to decay faster than the intensity of waves polarized along and perpendicular to the fibers.

Efficiency and cross-correlation in equity market during global financial crisis: Evidence from China

Science.gov (United States)

Ma, Pengcheng; Li, Daye; Li, Shuo

2016-02-01

Using one minute high-frequency data of the Shanghai Composite Index (SHCI) and the Shenzhen Composite Index (SZCI) (2007-2008), we employ the detrended fluctuation analysis (DFA) and the detrended cross correlation analysis (DCCA) with rolling window approach to observe the evolution of market efficiency and cross-correlation in pre-crisis and crisis period. Considering the fat-tail distribution of return time series, statistical test based on shuffling method is conducted to verify the null hypothesis of no long-term dependence. Our empirical research displays three main findings. First Shanghai equity market efficiency deteriorated while Shenzhen equity market efficiency improved with the advent of financial crisis. Second the highly positive dependence between SHCI and SZCI varies with time scale. Third financial crisis saw a significant increase of dependence between SHCI and SZCI at shorter time scales but a lack of significant change at longer time scales, providing evidence of contagion and absence of interdependence during crisis.

Local mobility in lipid domains of supported bilayers characterized by atomic force microscopy and fluorescence correlation spectroscopy.

Energy Technology Data Exchange (ETDEWEB)

Frankel, Daniel J.; Buranda, T. (University of New Mexico, Albuquerque, NM); Burns, Alan Richard

2005-01-01

Fluorescence correlation spectroscopy (FCS) is used to examine mobility of labeled probes at specific sites in supported bilayers consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid domains in 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). Those sites are mapped beforehand with simultaneous atomic force microscopy and submicron confocal fluorescence imaging, allowing characterization of probe partitioning between gel DPPC and disordered liquid DOPC domains with corresponding topography of domain structure. We thus examine the relative partitioning and mobility in gel and disordered liquid phases for headgroup- and tailgroup-labeled GM1 ganglioside probes and for headgroup- and tailgroup-labeled phospholipid probes. For the GM1 probes, large differences in mobility between fluid and gel domains are observed; whereas unexpected mobility is observed in submicron gel domains for the phospholipid probes. We attribute the latter to domain heterogeneities that could be induced by the probe. Furthermore, fits to the FCS data for the phospholipid probes in the DOPC fluid phase require two components (fast and slow). Although proximity to the glass substrate may be a factor, local distortion of the probe by the fluorophore could also be important. Overall, we observe nonideal aspects of phospholipid probe mobility and partitioning that may not be restricted to supported bilayers.

Measurement of OH free radical in magnetized sheet plasma crossed with vertical gas-flow by laser-induced fluorescence spectroscopy

International Nuclear Information System (INIS)

Tonegawa, Akira; Takatori, Masahiko; Kobayashi, Yukihiro; Kawamura, Kazutaka; Takayama, Kazuo

1997-01-01

We demonstrated the production of OH free radicals in an argon magnetized sheet plasma crossed with vertical gas-flow mixed with an oxygen gas O 2 and a hydrogen gas H 2 . The density and the rotational-vibrational temperature of the OH free radicals were measured by a laser-induced fluorescence (LIF). The density of OH free radicals increases with increasing O 2 gas-flow, while the high energy part of the electron-energy-distribution-function f e (E) above 8 eV decreases. These results suggest the high energy part of f e (E) is contributed to the dissociation of O 2 and the production of OH free radicals. (author)

Antinuclear antibodies: clinical significance of fluorescence patterns

International Nuclear Information System (INIS)

Cordeiro, S.L.; Habermann, F.; Franco, M.F.

1981-01-01

Fifty-four patients with 212 sera positive for antinuclear antibodies (ANA) were studied to: 1) determine the immunofluorescent nuclear staining patterns using Burnham's technique and simplified classification; 2) note the specificity of fluorescence patterns among the various connective tissue diseases; 3) study comparatively the fluorescence paterns employing 5 different antigen substrates; 4) correlate ANA titers and fluorescence patterns with renal involvement in systemic lupus erythematosus (SLE). It was observed: 1) most of the sera gave nonparticulate fluorescent patterns: peripheral, homogeneous, or peripheral-homogeneneous; 2) 55,5% of the patients had LE and most of those sera showed nonparticulate fluorescent patterns; 3) the sera displayed no specificity for any of the following antigen substrates: imprints of human normal spleen, frozen rat liver and kidney sections, frozen mouse kidney sections and perypheral human blood smears; 4) imprints of normal human spleen were the best substrate for accurate identification of fluorescent patterns; 5) sera from SLE patients with renal involvement showed higher ANA titers in relation to patients without renal involvement; both groups of sera gave similar ANA fluorescent patterns. (Author) [pt

Co-localized or randomly distributed? Pair cross correlation of in vivo grown subgingival biofilm bacteria quantified by digital image analysis.

Directory of Open Access Journals (Sweden)

Claudia Schillinger

Full Text Available The polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. A number of these microorganisms have been demonstrated in vitro to interact and enhance biofilm integration, survival or even pathogenic features. To examine the in vivo relevance of these proposed interactions, we extended the spatial arrangement analysis tool of the software daime (digital image analysis in microbial ecology. This modification enabled the quantitative analysis of microbial co-localization in images of subgingival biofilm species, where the biomass was confined to fractions of the whole-image area, a situation common for medical samples. Selected representatives of the disease-associated red and orange complexes that were previously suggested to interact with each other in vitro (Tannerella forsythia with Fusobacterium nucleatum and Porphyromonas gingivalis with Prevotella intermedia were chosen for analysis and labeled with specific fluorescent probes via fluorescence in situ hybridization. Pair cross-correlation analysis of in vivo grown biofilms revealed tight clustering of F. nucleatum/periodonticum and T. forsythia at short distances (up to 6 µm with a pronounced peak at 1.5 µm. While these results confirmed previous in vitro observations for F. nucleatum and T. forsythia, random spatial distribution was detected between P. gingivalis and P. intermedia in the in vivo samples. In conclusion, we successfully employed spatial arrangement analysis on the single cell level in clinically relevant medical samples and demonstrated the utility of this approach for the in vivo validation of in vitro observations by analyzing statistically relevant numbers of different patients. More importantly, the culture-independent nature of this approach enables similar quantitative analyses for "as

A Cross-Sectional Study Examining Youth Smoking Rates and Correlates in Tbilisi, Georgia

Directory of Open Access Journals (Sweden)

Carla J. Berg

2014-01-01

Full Text Available Georgia has high smoking rates; however, little is known about the prevalence and correlates of youth smoking. We conducted a secondary data analysis of a 2010 cross-sectional survey of 1,879 secondary and postsecondary school students aged 15 to 24 years in Tbilisi, Georgia, examining substance use, perceived risk, and recreational activities in relation to lifetime and current (past 30 days smoking. Lifetime and current smoking prevalence was 46.1% and 22.6%, respectively. In secondary schools, lifetime smoking correlates included being male, consuming alcohol, lifetime marijuana use, and lower perceived risk (P’s ≤ .001. Correlates of current smoking among lifetime smokers included being male, consuming alcohol, lifetime marijuana use, lower perceived risk, less frequently exercise, and more often going out (P’s < .05. In postsecondary schools, lifetime smoking correlates included being male, consuming alcohol, lifetime marijuana use, lower perceived risk, more often going out, and recreational internet use (P’s < .0. Correlates of current smoking among lifetime smokers included being male (P’s = .04, consuming alcohol, marijuana use, lower perceived risk, and more often going out (P’s < .05. Tobacco control interventions might target these correlates to reduce smoking prevalence in Georgian youth.

Dangers and uses of cross-correlation in analyzing time series in perception, performance, movement, and neuroscience: The importance of constructing transfer function autoregressive models.

Science.gov (United States)

Dean, Roger T; Dunsmuir, William T M

2016-06-01

Many articles on perception, performance, psychophysiology, and neuroscience seek to relate pairs of time series through assessments of their cross-correlations. Most such series are individually autocorrelated: they do not comprise independent values. Given this situation, an unfounded reliance is often placed on cross-correlation as an indicator of relationships (e.g., referent vs. response, leading vs. following). Such cross-correlations can indicate spurious relationships, because of autocorrelation. Given these dangers, we here simulated how and why such spurious conclusions can arise, to provide an approach to resolving them. We show that when multiple pairs of series are aggregated in several different ways for a cross-correlation analysis, problems remain. Finally, even a genuine cross-correlation function does not answer key motivating questions, such as whether there are likely causal relationships between the series. Thus, we illustrate how to obtain a transfer function describing such relationships, informed by any genuine cross-correlations. We illustrate the confounds and the meaningful transfer functions by two concrete examples, one each in perception and performance, together with key elements of the R software code needed. The approach involves autocorrelation functions, the establishment of stationarity, prewhitening, the determination of cross-correlation functions, the assessment of Granger causality, and autoregressive model development. Autocorrelation also limits the interpretability of other measures of possible relationships between pairs of time series, such as mutual information. We emphasize that further complexity may be required as the appropriate analysis is pursued fully, and that causal intervention experiments will likely also be needed.

Big Data solution for CTBT monitoring: CEA-IDC joint global cross correlation project

Science.gov (United States)

Bobrov, Dmitry; Bell, Randy; Brachet, Nicolas; Gaillard, Pierre; Kitov, Ivan; Rozhkov, Mikhail

2014-05-01

Waveform cross-correlation when applied to historical datasets of seismic records provides dramatic improvements in detection, location, and magnitude estimation of natural and manmade seismic events. With correlation techniques, the amplitude threshold of signal detection can be reduced globally by a factor of 2 to 3 relative to currently standard beamforming and STA/LTA detector. The gain in sensitivity corresponds to a body wave magnitude reduction by 0.3 to 0.4 units and doubles the number of events meeting high quality requirements (e.g. detected by three and more seismic stations of the International Monitoring System (IMS). This gain is crucial for seismic monitoring under the Comprehensive Nuclear-Test-Ban Treaty. The International Data Centre (IDC) dataset includes more than 450,000 seismic events, tens of millions of raw detections and continuous seismic data from the primary IMS stations since 2000. This high-quality dataset is a natural candidate for an extensive cross correlation study and the basis of further enhancements in monitoring capabilities. Without this historical dataset recorded by the permanent IMS Seismic Network any improvements would not be feasible. However, due to the mismatch between the volume of data and the performance of the standard Information Technology infrastructure, it becomes impossible to process all the data within tolerable elapsed time. To tackle this problem known as "BigData", the CEA/DASE is part of the French project "DataScale". One objective is to reanalyze 10 years of waveform data from the IMS network with the cross-correlation technique thanks to a dedicated High Performance Computer (HPC) infrastructure operated by the Centre de Calcul Recherche et Technologie (CCRT) at the CEA of Bruyères-le-Châtel. Within 2 years we are planning to enhance detection and phase association algorithms (also using machine learning and automatic classification) and process about 30 terabytes of data provided by the IDC to

Robust alignment of chromatograms by statistically analyzing the shifts matrix generated by moving window fast Fourier transform cross-correlation.

Science.gov (United States)

Zhang, Mingjing; Wen, Ming; Zhang, Zhi-Min; Lu, Hongmei; Liang, Yizeng; Zhan, Dejian

2015-03-01

Retention time shift is one of the most challenging problems during the preprocessing of massive chromatographic datasets. Here, an improved version of the moving window fast Fourier transform cross-correlation algorithm is presented to perform nonlinear and robust alignment of chromatograms by analyzing the shifts matrix generated by moving window procedure. The shifts matrix in retention time can be estimated by fast Fourier transform cross-correlation with a moving window procedure. The refined shift of each scan point can be obtained by calculating the mode of corresponding column of the shifts matrix. This version is simple, but more effective and robust than the previously published moving window fast Fourier transform cross-correlation method. It can handle nonlinear retention time shift robustly if proper window size has been selected. The window size is the only one parameter needed to adjust and optimize. The properties of the proposed method are investigated by comparison with the previous moving window fast Fourier transform cross-correlation and recursive alignment by fast Fourier transform using chromatographic datasets. The pattern recognition results of a gas chromatography mass spectrometry dataset of metabolic syndrome can be improved significantly after preprocessing by this method. Furthermore, the proposed method is available as an open source package at https://github.com/zmzhang/MWFFT2. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

What is new about covered interest parity condition in the European Union? Evidence from fractal cross-correlation regressions

Science.gov (United States)

Ferreira, Paulo; Kristoufek, Ladislav

2017-11-01

We analyse the covered interest parity (CIP) using two novel regression frameworks based on cross-correlation analysis (detrended cross-correlation analysis and detrending moving-average cross-correlation analysis), which allow for studying the relationships at different scales and work well under non-stationarity and heavy tails. CIP is a measure of capital mobility commonly used to analyse financial integration, which remains an interesting feature of study in the context of the European Union. The importance of this features is related to the fact that the adoption of a common currency is associated with some benefits for countries, but also involves some risks such as the loss of economic instruments to face possible asymmetric shocks. While studying the Eurozone members could explain some problems in the common currency, studying the non-Euro countries is important to analyse if they are fit to take the possible benefits. Our results point to the CIP verification mainly in the Central European countries while in the remaining countries, the verification of the parity is only residual.

Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy

Directory of Open Access Journals (Sweden)

Akira Kitamura

2018-07-01

Full Text Available Normal function and abnormal aggregation of transactivation response (TAR DNA/RNA-binding protein 43 kDa (TDP-43 are directly associated with the lethal genetic diseases: cystic fibrosis, amyotrophic lateral sclerosis (ALS, and frontotemporal lobar degeneration (FTLD. The binding of TDP-43 to single-stranded DNA (ssDNA or RNA is involved in transcriptional repression, regulation of RNA splicing, and RNA stabilization. Equilibrium dissociation constants (Kd of TDP-43 and ssDNA or RNA have been determined using various methods; however, methods that can measure Kd with high sensitivity in a short time using a small amount of TDP-43 in solution would be advantageous. Here, in order to determine the Kd of TDP-43 and fluorescence-labeled ssDNA as well as the binding stoichiometry, we use fluorescence correlation spectroscopy (FCS, which detects the slowed diffusion of molecular interactions in solution with single-molecule sensitivity, in addition to electrophoretic mobility shift assay (EMSA. Using tandem affinity chromatography of TDP-43 dually tagged with glutathione-S-transferase and poly-histidine tags, highly purified protein was obtained. FCS successfully detected specific interaction between purified TDP-43 and TG ssDNA repeats, with a Kd in the nanomolar range. The Kd of the TDP-43 mutant was not different from the wild type, although mutant oligomers, which did not bind ssDNA, were observed. Analysis of the fluorescence brightness per dimerized TDP-43/ssDNA complex was used to evaluate their binding stoichiometry. The results suggest that an assay combining FCS and EMSA can precisely analyze ssDNA recognition mechanisms, and that FCS may be applied for the rapid and quantitative determination of the interaction strength between TDP-43 and ssDNA or RNA. These methods will aid in the elucidation of the substrate recognition mechanism of ALS- and FTLD-associated variants of TDP-43.

Study of the correlation between the coal calorific value and coal ash content using X-ray fluorescence analysis

International Nuclear Information System (INIS)

Bolortuya, D.; Zuzaan, P.; Gustova, M.V.; Maslov, O.D.

2013-01-01

In this paper we have studied the possibility of determining the chemical elements in coal samples using X-ray fluorescence analysis and have found a relationship between the coal calorific value and its ash content with the coal moisture accounting. The amount of coal ash can be determined by the content of the basic chemical elements, such as Si, Sr, Fe, and Ca. It was concluded that the calorific value of coal can be estimated from the ash content in coal without the calorimetric measurements. These correlation coefficients were calculated for several coal mines in Mongolia. The results are in good agreement with the results of chemical analysis

Detrended cross-correlation coefficient: Application to predict apoptosis protein subcellular localization.

Science.gov (United States)

Liang, Yunyun; Liu, Sanyang; Zhang, Shengli

2016-12-01

Apoptosis, or programed cell death, plays a central role in the development and homeostasis of an organism. Obtaining information on subcellular location of apoptosis proteins is very helpful for understanding the apoptosis mechanism. The prediction of subcellular localization of an apoptosis protein is still a challenging task, and existing methods mainly based on protein primary sequences. In this paper, we introduce a new position-specific scoring matrix (PSSM)-based method by using detrended cross-correlation (DCCA) coefficient of non-overlapping windows. Then a 190-dimensional (190D) feature vector is constructed on two widely used datasets: CL317 and ZD98, and support vector machine is adopted as classifier. To evaluate the proposed method, objective and rigorous jackknife cross-validation tests are performed on the two datasets. The results show that our approach offers a novel and reliable PSSM-based tool for prediction of apoptosis protein subcellular localization. Copyright © 2016 Elsevier Inc. All rights reserved.

Differentiation of Cariogenic Streptococci by Fluorescent Antibody1

Science.gov (United States)

Jablon, James M.; Zinner, Doran D.

1966-01-01

Jablon, J. M. (University of Miami, Miami, Fla.), and D. D. Zinner. Differentiation of cariogenic streptococci by fluorescent antibody. J. Bacteriol. 92:1590–1596. 1966.—Eight strains of streptococci were isolated from human carious lesions by the fluorescent-antibody (FA) technique. Seven of these strains produced experimental caries in hamsters or rats maintained on a high sucrose diet. The eighth strain was noncariogenic in animals but possessed some antigenic components in common with the cariogenic strains. On the basis of antigen-antibody reactions by microprecipitin and agar-gel diffusion patterns, the strains were divided into four groups; these groups differed with regard to their cariogenic activity in hamsters. Fluorescein-conjugated antisera, prepared against the human strains, showed some cross-reactions which interfered with the efficacy of the FA technique in differentiating between the related streptococcal groups. To eliminate these cross-reactions, a small amount of related-strain antisera was added to the fluorescein-conjugated antisera to the cariogenic strains. This technique is effective in blocking cross-reactions and should be tried wherever cross-reactions are encountered in the FA technique. Images PMID:5334765

Estimation of velocity vector angles using the directional cross-correlation method

DEFF Research Database (Denmark)

Kortbek, Jacob; Jensen, Jørgen Arendt

2006-01-01

and then select the angle with the highest normalized correlation between directional signals. The approach is investigated using Field II simulations and data from the experimental ultrasound scanner RASMUS and a circulating flow rig with a parabolic flow having a peak velocity of 0.3 m/s. A 7 MHz linear array......A method for determining both velocity magnitude and angle in any direction is suggested. The method uses focusing along the velocity direction and cross-correlation for finding the correct velocity magnitude. The angle is found from beamforming directional signals in a number of directions...... transducer is used with a normal transmission of a focused ultrasound field. In the simulations the relative standard deviation of the velocity magnitude is between 0.7% and 7.7% for flow angles between 45 deg and 90 deg. The study showed that angle estimation by directional beamforming can be estimated...

Quasar-Lyman α forest cross-correlation from BOSS DR11: Baryon Acoustic Oscillations

Energy Technology Data Exchange (ETDEWEB)

Font-Ribera, Andreu [Institute of Theoretical Physics, University of Zurich, Winterthurerstrasse 190, 8057 Zurich (Switzerland); Kirkby, David; Blomqvist, Michael [Department of Physics and Astronomy, University of California, 4129 Frederick Reines Hall, Irvine, CA, 92697 (United States); Busca, Nicolas; Aubourg, Éric; Bautista, Julian [APC, Université Paris Diderot-Paris 7, CNRS/IN2P3, CEA, Observatoire de Paris, 10, rue A. Domon and L. Duquet, Paris (France); Miralda-Escudé, Jordi [Institut de Ciències del Cosmos (IEEC/UB), Martí i Franquès 1, Barcelona, 08028 Catalonia (Spain); Ross, Nicholas P.; Bailey, Stephen; Beutler, Florian; Carithers, Bill [Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA (United States); Slosar, Anže [Brookhaven National Laboratory, Blgd 510, Upton, NY, 11375 (United States); Rich, James; Delubac, Timothée [CEA, Centre de Saclay, IRFU, Gif-sur-Yvette, 91191 France (France); Bhardwaj, Vaishali; Bizyaev, Dmitry [Department of Astronomy, University of Washington, Box 351580, Seattle, WA, 98195 (United States); Brewington, Howard; Brinkmann, Jon [Apache Point Observatory and New Mexico State University, P.O. Box 59, Sunspot, NM, 88349-0059 (United States); Brownstein, Joel R.; Dawson, Kyle S., E-mail: font@physik.uzh.ch [Department of Physics and Astronomy, University of Utah, 115 S 1400 E, Salt Lake City, UT, 84112 (United States); and others

2014-05-01

We measure the large-scale cross-correlation of quasars with the Lyα forest absorption, using over 164,000 quasars from Data Release 11 of the SDSS-III Baryon Oscillation Spectroscopic Survey. We extend the previous study of roughly 60,000 quasars from Data Release 9 to larger separations, allowing a measurement of the Baryonic Acoustic Oscillation (BAO) scale along the line of sight c/(H(z = 2.36)r{sub s}) = 9.0±0.3 and across the line of sight D{sub A}(z = 2.36)/r{sub s} = 10.8±0.4, consistent with CMB and other BAO data. Using the best fit value of the sound horizon from Planck data (r{sub s} = 147.49 Mpc), we can translate these results to a measurement of the Hubble parameter of H(z = 2.36) = 226±8 km s{sup −1} Mpc{sup −1} and of the angular diameter distance of D{sub A}(z = 2.36) = 1590±60 Mpc. The measured cross-correlation function and an update of the code to fit the BAO scale (baofit) are made publicly available.

Conformational fluctuation dynamics of domain I of human serum albumin in the course of chemically and thermally induced unfolding using fluorescence correlation spectroscopy.

Science.gov (United States)

Yadav, Rajeev; Sengupta, Bhaswati; Sen, Pratik

2014-05-22

The present study elucidates the involvement of conformational fluctuation dynamics during chemically and thermally induced unfolding of human serum albumin (HSA) by fluorescence correlation spectroscopic (FCS) study, time-resolved fluorescence measurements, and circular dichroism (CD) spectroscopic methods. Two fluorescent probes, tetramethylrhodamine-5-maleimide (TMR) and N-(7-dimethylamino-4-methylcoumarin-3-yl) iodoacetamide (DACIA) were used to selectively label the domain I of HSA through the reaction with cys-34 for these studies. The guanidine hydrochloride (GnHCl) induced global structural change of HSA is monitored through its hydrodynamic radius (r(H)) and CD response, which is found to be two step in nature. In FCS experiment, along with the diffusion time component we have observed an exponential relaxation time component (τ(R)) that has been ascribed to the concerted chain dynamics of HSA. Unlike in the global structural change, we found that the τ(R) value changes in a different manner in the course of the unfolding. The dependence of τ(R) on the concentration of GnHCl was best fitted with a four state model, indicating the involvement of two intermediate states during the unfolding process, which were not observed through the CD response and r(H) data. The fluorescence lifetime measurement also supports our observation of intermediate states during the unfolding of HSA. However, no such intermediate states were observed during thermally induced unfolding of HSA.

Blind Cartography for Side Channel Attacks: Cross-Correlation Cartography

Directory of Open Access Journals (Sweden)

Laurent Sauvage

2012-01-01

Full Text Available Side channel and fault injection attacks are major threats to cryptographic applications of embedded systems. Best performances for these attacks are achieved by focusing sensors or injectors on the sensible parts of the application, by means of dedicated methods to localise them. Few methods have been proposed in the past, and all of them aim at pinpointing the cryptoprocessor. However it could be interesting to exploit the activity of other parts of the application, in order to increase the attack's efficiency or to bypass its countermeasures. In this paper, we present a localisation method based on cross-correlation, which issues a list of areas of interest within the attacked device. It realizes an exhaustive analysis, since it may localise any module of the device, and not only those which perform cryptographic operations. Moreover, it also does not require a preliminary knowledge about the implementation, whereas some previous cartography methods require that the attacker could choose the cryptoprocessor inputs, which is not always possible. The method is experimentally validated using observations of the electromagnetic near field distribution over a Xilinx Virtex 5 FPGA. The matching between areas of interest and the application layout in the FPGA floorplan is confirmed by correlation analysis.

Improving ambient noise cross-correlations in the noisy ocean bottom environment of the Juan de Fuca plate

Science.gov (United States)

Tian, Ye; Ritzwoller, Michael H.

2017-09-01

Ambient noise tomography exploits seismic ground motions that propagate coherently over long interstation distances. Such ground motions provide information about the medium of propagation that is recoverable from interstation cross-correlations. Local noise sources, which are particularly strong in ocean bottom environments, corrupt ambient noise cross-correlations and compromise the effectiveness of ambient noise tomography. Based on 62 ocean bottom seismometers (OBSs) located on Juan de Fuca (JdF) plate from the Cascadia Initiative experiment and 40 continental stations near the coast of the western United States obtained in 2011 and 2012, we attempt to reduce the effects of local noise on vertical component seismic records across the plate and onto US continent. The goal is to provide better interstation cross-correlations for use in ambient noise tomography and the study of ambient noise directionality. As shown in previous studies, tilt and compliance noise are major sources of noise that contaminate the vertical channels of the OBSs and such noise can be greatly reduced by exploiting information on the horizontal components and the differential pressure gauge records, respectively. We find that ambient noise cross-correlations involving OBSs are of significantly higher signal-to-noise ratio at periods greater than 10 s after reducing these types of noise, particularly in shallow water environments where tilt and compliance noise are especially strong. The reduction of tilt and compliance noise promises to improve the accuracy and spatial extent of ambient noise tomography, allowing measurements based on coherently propagating ambient noise to be made at stations in the shallower parts of the JdF plate and at longer periods than in previous studies. In addition such local noise reduction produces better estimates of the azimuthal content of ambient noise.

Photonic reagents for concentration measurement of flu-orescent proteins with overlapping spectra

Science.gov (United States)

Goun, Alexei; Bondar, Denys I.; Er, Ali O.; Quine, Zachary; Rabitz, Herschel A.

2016-05-01

By exploiting photonic reagents (i.e., coherent control by shaped laser pulses), we employ Optimal Dynamic Discrimination (ODD) as a novel means for quantitatively characterizing mixtures of fluorescent proteins with a large spectral overlap. To illustrate ODD, we simultaneously measured concentrations of in vitro mixtures of Enhanced Blue Fluorescent Protein (EBFP) and Enhanced Cyan Fluorescent Protein (ECFP). Building on this foundational study, the ultimate goal is to exploit the capabilities of ODD for parallel monitoring of genetic and protein circuits by suppressing the spectral cross-talk among multiple fluorescent reporters.

Dynamic Hebbian Cross-Correlation Learning Resolves the Spike Timing Dependent Plasticity Conundrum

Directory of Open Access Journals (Sweden)

Tjeerd V. olde Scheper

2018-01-01

Full Text Available Spike Timing-Dependent Plasticity has been found to assume many different forms. The classic STDP curve, with one potentiating and one depressing window, is only one of many possible curves that describe synaptic learning using the STDP mechanism. It has been shown experimentally that STDP curves may contain multiple LTP and LTD windows of variable width, and even inverted windows. The underlying STDP mechanism that is capable of producing such an extensive, and apparently incompatible, range of learning curves is still under investigation. In this paper, it is shown that STDP originates from a combination of two dynamic Hebbian cross-correlations of local activity at the synapse. The correlation of the presynaptic activity with the local postsynaptic activity is a robust and reliable indicator of the discrepancy between the presynaptic neuron and the postsynaptic neuron's activity. The second correlation is between the local postsynaptic activity with dendritic activity which is a good indicator of matching local synaptic and dendritic activity. We show that this simple time-independent learning rule can give rise to many forms of the STDP learning curve. The rule regulates synaptic strength without the need for spike matching or other supervisory learning mechanisms. Local differences in dendritic activity at the synapse greatly affect the cross-correlation difference which determines the relative contributions of different neural activity sources. Dendritic activity due to nearby synapses, action potentials, both forward and back-propagating, as well as inhibitory synapses will dynamically modify the local activity at the synapse, and the resulting STDP learning rule. The dynamic Hebbian learning rule ensures furthermore, that the resulting synaptic strength is dynamically stable, and that interactions between synapses do not result in local instabilities. The rule clearly demonstrates that synapses function as independent localized

CALIBRATING PHOTOMETRIC REDSHIFT DISTRIBUTIONS WITH CROSS-CORRELATIONS

International Nuclear Information System (INIS)

Schulz, A. E.

2010-01-01

The next generation of proposed galaxy surveys will increase the number of galaxies with photometric redshift identifications by two orders of magnitude, drastically expanding both the redshift range and detection threshold from the current state of the art. Obtaining spectra for a fair subsample of these new data could be cumbersome and expensive. However, adequate calibration of the true redshift distribution of galaxies is vital to tapping the potential of these surveys to illuminate the processes of galaxy evolution and to constrain the underlying cosmology and growth of structure. We examine here an alternative to direct spectroscopic follow-up: calibration of the redshift distribution of photometric galaxies via cross-correlation with an overlapping spectroscopic survey whose members trace the same density field. We review the theory, develop a pipeline to implement the method, apply it to mock data from N-body simulations, and examine the properties of this redshift distribution estimator. We demonstrate that the method is generally effective, but the estimator is weakened by two main factors. One is that the correlation function of the spectroscopic sample must be measured in many bins along the line of sight, which renders the measurement noisy and interferes with high-quality reconstruction of the photometric redshift distribution. Also, the method is not able to disentangle the photometric redshift distribution from redshift dependence in the bias of the photometric sample. We establish the impact of these factors using our mock catalogs. We conclude that it may still be necessary to spectroscopically follow up a fair subsample of the photometric survey data. Nonetheless, it is significant that the method has been successfully implemented on mock data, and with further refinement it may appreciably decrease the number of spectra that will be needed to calibrate future surveys.

An overview of remote sensing of chlorophyll fluorescence

Science.gov (United States)

Xing, Xiao-Gang; Zhao, Dong-Zhi; Liu, Yu-Guang; Yang, Jian-Hong; Xiu, Peng; Wang, Lin

2007-03-01

Besides empirical algorithms with the blue-green ratio, the algorithms based on fluorescence are also important and valid methods for retrieving chlorophyll-a concentration in the ocean waters, especially for Case II waters and the sea with algal blooming. This study reviews the history of initial cognitions, investigations and detailed approaches towards chlorophyll fluorescence, and then introduces the biological mechanism of fluorescence remote sensing and main spectral characteristics such as the positive correlation between fluorescence and chlorophyll concentration, the red shift phenomena. Meanwhile, there exist many influence factors that increase complexity of fluorescence remote sensing, such as fluorescence quantum yield, physiological status of various algae, substances with related optical property in the ocean, atmospheric absorption etc. Based on these cognitions, scientists have found two ways to calculate the amount of fluorescence detected by ocean color sensors: fluorescence line height and reflectance ratio. These two ways are currently the foundation for retrieval of chlorophyl l - a concentration in the ocean. As the in-situ measurements and synchronous satellite data are continuously being accumulated, the fluorescence remote sensing of chlorophyll-a concentration in Case II waters should be recognized more thoroughly and new algorithms could be expected.

Collisional and radiative processes in fluorescent lamps

International Nuclear Information System (INIS)

Lister, Graeme G.

2003-01-01

Since electrode life is the major limiting factor in operating fluorescent lamps, many lighting companies have introduced 'electrodeless' fluorescent lamps, using inductively coupled discharges. These lamps often operate at much higher power loadings than standard lamps and numerical models have not been successful in reproducing experimental measurements in the parameter ranges of interest. A comprehensive research program was undertaken to study the fundamental physical processes of these discharges, co-funded by the Electric Power Research Institute (EPRI) and OSRAM SYLVANIA under the name of ALITE. The program included experiments and modeling of radiation transport, computations of electron-atom and atom-atom cross sections and the first comprehensive power balance studies of a highly loaded fluorescent lamp. Results from the program and their importance to the understanding of the physics of fluorescent lamps are discussed, with particular emphasis on the important collisional and radiative processes. Comparisons between results of experimental measurements and numerical models are presented

Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

Czech Academy of Sciences Publication Activity Database

Macháň, Radek; Hof, Martin

2010-01-01

Roč. 1798, č. 7 (2010), s. 1377-1391 ISSN 0005-2736 R&D Projects: GA ČR GA203/08/0114; GA AV ČR GEMEM/09/E006 Institutional research plan: CEZ:AV0Z40400503 Keywords : supported lipid bilayer * giant unilamellar vesicle * fluorescence recovery after photobleaching Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 4.647, year: 2010

Self-organising maps and correlation analysis as a tool to explore patterns in excitation-emission matrix data sets and to discriminate dissolved organic matter fluorescence components.

Science.gov (United States)

Ejarque-Gonzalez, Elisabet; Butturini, Andrea

2014-01-01

Dissolved organic matter (DOM) is a complex mixture of organic compounds, ubiquitous in marine and freshwater systems. Fluorescence spectroscopy, by means of Excitation-Emission Matrices (EEM), has become an indispensable tool to study DOM sources, transport and fate in aquatic ecosystems. However the statistical treatment of large and heterogeneous EEM data sets still represents an important challenge for biogeochemists. Recently, Self-Organising Maps (SOM) has been proposed as a tool to explore patterns in large EEM data sets. SOM is a pattern recognition method which clusterizes and reduces the dimensionality of input EEMs without relying on any assumption about the data structure. In this paper, we show how SOM, coupled with a correlation analysis of the component planes, can be used both to explore patterns among samples, as well as to identify individual fluorescence components. We analysed a large and heterogeneous EEM data set, including samples from a river catchment collected under a range of hydrological conditions, along a 60-km downstream gradient, and under the influence of different degrees of anthropogenic impact. According to our results, chemical industry effluents appeared to have unique and distinctive spectral characteristics. On the other hand, river samples collected under flash flood conditions showed homogeneous EEM shapes. The correlation analysis of the component planes suggested the presence of four fluorescence components, consistent with DOM components previously described in the literature. A remarkable strength of this methodology was that outlier samples appeared naturally integrated in the analysis. We conclude that SOM coupled with a correlation analysis procedure is a promising tool for studying large and heterogeneous EEM data sets.

Understanding volatility correlation behavior with a magnitude cross-correlation function

Science.gov (United States)

Jun, Woo Cheol; Oh, Gabjin; Kim, Seunghwan

2006-06-01

We propose an approach for analyzing the basic relation between correlation properties of the original signal and its magnitude fluctuations by decomposing the original signal into its positive and negative fluctuation components. We use this relation to understand the following phenomenon found in many naturally occurring time series: the magnitude of the signal exhibits long-range correlation, whereas the original signal is short-range correlated. The applications of our approach to heart rate variability signals and high-frequency foreign exchange rates reveal that the difference between the correlation properties of the original signal and its magnitude fluctuations is induced by the time organization structure of the correlation function between the magnitude fluctuations of positive and negative components. We show that this correlation function can be described well by a stretched-exponential function and is related to the nonlinearity and the multifractal structure of the signals.

Understanding volatility correlation behavior with a magnitude cross-correlation function.

Science.gov (United States)

Jun, Woo Cheol; Oh, Gabjin; Kim, Seunghwan

2006-06-01

We propose an approach for analyzing the basic relation between correlation properties of the original signal and its magnitude fluctuations by decomposing the original signal into its positive and negative fluctuation components. We use this relation to understand the following phenomenon found in many naturally occurring time series: the magnitude of the signal exhibits long-range correlation, whereas the original signal is short-range correlated. The applications of our approach to heart rate variability signals and high-frequency foreign exchange rates reveal that the difference between the correlation properties of the original signal and its magnitude fluctuations is induced by the time organization structure of the correlation function between the magnitude fluctuations of positive and negative components. We show that this correlation function can be described well by a stretched-exponential function and is related to the nonlinearity and the multifractal structure of the signals.

When can Empirical Green Functions be computed from Noise Cross-Correlations? Hints from different Geographical and Tectonic environments

Science.gov (United States)

Matos, Catarina; Silveira, Graça; Custódio, Susana; Domingues, Ana; Dias, Nuno; Fonseca, João F. B.; Matias, Luís; Krueger, Frank; Carrilho, Fernando

2014-05-01

Noise cross-correlations are now widely used to extract Green functions between station pairs. But, do all the cross-correlations routinely computed produce successful Green Functions? What is the relationship between noise recorded in a couple of stations and the cross-correlation between them? During the last decade, we have been involved in the deployment of several temporary dense broadband (BB) networks within the scope of both national projects and international collaborations. From 2000 to 2002, a pool of 8 BB stations continuously operated in the Azores in the scope of the Memorandum of Understanding COSEA (COordinated Seismic Experiment in the Azores). Thanks to the Project WILAS (West Iberia Lithosphere and Astenosphere Structure, PTDC/CTE-GIX/097946/2008) we temporarily increased the number of BB deployed in mainland Portugal to more than 50 (permanent + temporary) during the period 2010 - 2012. In 2011/12 a temporary pool of 12 seismometers continuously recorded BB data in the Madeira archipelago, as part of the DOCTAR (Deep Ocean Test Array Experiment) project. Project CV-PLUME (Investigation on the geometry and deep signature of the Cape Verde mantle plume, PTDC/CTE-GIN/64330/2006) covered the archipelago of Cape Verde, North Atlantic, with 40 temporary BB stations in 2007/08. Project MOZART (Mozambique African Rift Tomography, PTDC/CTE-GIX/103249/2008), covered Mozambique, East Africa, with 30 temporary BB stations in the period 2011 - 2013. These networks, located in very distinct geographical and tectonic environments, offer an interesting opportunity to study seasonal and spatial variations of noise sources and their impact on Empirical Green functions computed from noise cross-correlation. Seismic noise recorded at different seismic stations is evaluated by computation of the probability density functions of power spectral density (PSD) of continuous data. To assess seasonal variations of ambient noise sources in frequency content, time-series of

Correlation between ultrasound imaging, cross-sectional anatomy, and histology of the brachial plexus: a review.

Science.gov (United States)

van Geffen, Geert J; Moayeri, Nizar; Bruhn, Jörgen; Scheffer, Gert J; Chan, Vincent W; Groen, Gerbrand J

2009-01-01

The anatomy of the brachial plexus is complex. To facilitate the understanding of the ultrasound appearance of the brachial plexus, we present a review of important anatomic considerations. A detailed correlation of reconstructed, cross-sectional gross anatomy and histology with ultrasound sonoanatomy is provided.

Effect of in-medium nucleon-nucleon cross section on proton-proton momentum correlation in intermediate-energy heavy-ion collisions

Science.gov (United States)

Wang, Ting-Ting; Ma, Yu-Gang; Zhang, Chun-Jian; Zhang, Zheng-Qiao

2018-03-01

The proton-proton momentum correlation function from different rapidity regions is systematically investigated for the Au + Au collisions at different impact parameters and different energies from 400 A MeV to 1500 A MeV in the framework of the isospin-dependent quantum molecular dynamics model complemented by the Lednický-Lyuboshitz analytical method. In particular, the in-medium nucleon-nucleon cross-section dependence of the correlation function is brought into focus, while the impact parameter and energy dependence of the momentum correlation function are also explored. The sizes of the emission source are extracted by fitting the momentum correlation functions using the Gaussian source method. We find that the in-medium nucleon-nucleon cross section obviously influences the proton-proton momentum correlation function, which is from the whole-rapidity or projectile or target rapidity region at smaller impact parameters, but there is no effect on the mid-rapidity proton-proton momentum correlation function, which indicates that the emission mechanism differs between projectile or target rapidity and mid-rapidity protons.

Method for stationarity-segmentation of spike train data with application to the Pearson cross-correlation.

Science.gov (United States)

Quiroga-Lombard, Claudio S; Hass, Joachim; Durstewitz, Daniel

2013-07-01

Correlations among neurons are supposed to play an important role in computation and information coding in the nervous system. Empirically, functional interactions between neurons are most commonly assessed by cross-correlation functions. Recent studies have suggested that pairwise correlations may indeed be sufficient to capture most of the information present in neural interactions. Many applications of correlation functions, however, implicitly tend to assume that the underlying processes are stationary. This assumption will usually fail for real neurons recorded in vivo since their activity during behavioral tasks is heavily influenced by stimulus-, movement-, or cognition-related processes as well as by more general processes like slow oscillations or changes in state of alertness. To address the problem of nonstationarity, we introduce a method for assessing stationarity empirically and then "slicing" spike trains into stationary segments according to the statistical definition of weak-sense stationarity. We examine pairwise Pearson cross-correlations (PCCs) under both stationary and nonstationary conditions and identify another source of covariance that can be differentiated from the covariance of the spike times and emerges as a consequence of residual nonstationarities after the slicing process: the covariance of the firing rates defined on each segment. Based on this, a correction of the PCC is introduced that accounts for the effect of segmentation. We probe these methods both on simulated data sets and on in vivo recordings from the prefrontal cortex of behaving rats. Rather than for removing nonstationarities, the present method may also be used for detecting significant events in spike trains.

A novel turn-on fluorescent strategy for sensing ascorbic acid using graphene quantum dots as fluorescent probe.

Science.gov (United States)

Liu, Hua; Na, Weidan; Liu, Ziping; Chen, Xueqian; Su, Xingguang

2017-06-15

In this paper, a facile and rapid fluorescence turn-on assay for fluorescent detection of ascorbic acid (AA) was developed by using the orange emission graphene quantum dots (GQDs). In the presence of horse radish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ), catechol can be oxidized by hydroxyl radicals and converted to o-benzoquinone, which can significantly quench the fluorescence of GQDs. However, when AA present in the system, it can consume part of H 2 O 2 and hydroxyl radicals to inhibit the generation of o-benzoquinone, resulting in fluorescence recovery. Under the optimized experimental conditions, the fluorescence intensity was linearly correlated with the concentration of H 2 O 2 in the range of 3.33-500µM with a detection limit of 1.2µM. The linear detection for AA was in the range from 1.11 to 300µM with a detection limit of 0.32µM. The proposed method was applied to the determination of AA in human serum samples with satisfactory results. Copyright © 2017. Published by Elsevier B.V.

Multiphoton-Excited Fluorescence of Silicon-Vacancy Color Centers in Diamond

Science.gov (United States)

Higbie, J. M.; Perreault, J. D.; Acosta, V. M.; Belthangady, C.; Lebel, P.; Kim, M. H.; Nguyen, K.; Demas, V.; Bajaj, V.; Santori, C.

2017-05-01

Silicon-vacancy color centers in nanodiamonds are promising as fluorescent labels for biological applications, with a narrow, nonbleaching emission line at 738 nm. Two-photon excitation of this fluorescence offers the possibility of low-background detection at significant tissue depth with high three-dimensional spatial resolution. We measure the two-photon fluorescence cross section of a negatively charged silicon vacancy (Si -V- ) in ion-implanted bulk diamond to be 0.74 (19 )×10-50 cm4 s /photon at an excitation wavelength of 1040 nm. Compared to the diamond nitrogen-vacancy center, the expected detection threshold of a two-photon excited Si -V center is more than an order of magnitude lower, largely due to its much narrower linewidth. We also present measurements of two- and three-photon excitation spectra, finding an increase in the two-photon cross section with decreasing wavelength, and we discuss the physical interpretation of the spectra in the context of existing models of the Si -V energy-level structure.

The use of yellow fluorescent hybrids to indicate mating in Trypanosoma brucei

Directory of Open Access Journals (Sweden)

Ferris Vanessa

2008-02-01

Full Text Available Abstract Background Trypanosoma brucei undergoes genetic exchange in its insect vector, the tsetse fly, by an unknown mechanism. The difficulties of working with this experimental system of genetic exchange have hampered investigation, particularly because the trypanosome life cycle stages involved cannot be cultured in vitro and therefore must be examined in the insect. Searching for small numbers of hybrid trypanosomes directly in the fly has become possible through the incorporation of fluorescent reporter genes, and we have previously carried out a successful cross using a reporter-repressor strategy. However, we could not be certain that all fluorescent trypanosomes observed in that cross were hybrids, due to mutations of the repressor leading to spontaneous fluorescence, and we have therefore developed an alternative strategy. Results To visualize the production of hybrids in the fly, parental trypanosome clones were transfected with a gene encoding Green Fluorescent Protein (GFP or Red Fluorescent Protein (RFP. Co-infection of flies with red and green fluorescent parental trypanosomes produced yellow fluorescent hybrids, which were easily visualized in the fly salivary glands. Yellow trypanosomes were not seen in midgut or proventricular samples and first appeared in the glands as epimastigotes as early as 13 days after fly infection. Cloned progeny originating from individual salivary glands had yellow, red, green or no fluorescence and were confirmed as hybrids by microsatellite, molecular karyotype and kinetoplast (mitochondrial DNA analyses. Hybrid clones showed biparental inheritance of both nuclear and kinetoplast genomes. While segregation and reassortment of the reporter genes and microsatellite alleles were consistent with Mendelian inheritance, flow cytometry measurement of DNA content revealed both diploid and polyploid trypanosomes among the hybrid progeny clones. Conclusion The strategy of using production of yellow hybrids

Inclusive D* Meson Cross Sections and D* Jet Correlations in Photoproduction at HERA

CERN Document Server

Aktas, A.; Anthonis, T.; Antunovic, B.; Aplin, S.; Asmone, A.; Astvatsatourov, A.; Babaev, A.; Backovic, S.; Baghdasaryan, A.; Baranov, P.; Barrelet, E.; Bartel, W.; Baudrand, S.; Baumgartner, S.; Beckingham, M.; Behnke, O.; Behrendt, O.; Belousov, A.; Berger, N.; Bizot, J.C.; Boenig, M.O.; Boudry, V.; Bracinik, J.; Brandt, G.; Brisson, V.; Bruncko, D.; Busser, F.W.; Bunyatyan, A.; Buschhorn, G.; Bystritskaya, L.; Campbell, A.J.; Caron, S.; Cassol-Brunner, F.; Cerny, K.; Cerny, V.; Chekelian, V.; Contreras, J.G.; Coughlan, J.A.; Cox, B.E.; Cozzika, G.; Cvach, J.; Dainton, J.B.; Dau, W.D.; Daum, K.; de Boer, Y.; Delcourt, B.; Del Degan, M.; De Roeck, A.; De Wolf, E.A.; Diaconu, C.; Dodonov, V.; Dubak, A.; Eckerlin, Guenter; Efremenko, V.; Egli, S.; Eichler, R.; Eisele, F.; Eliseev, A.; Elsen, E.; Essenov, S.; Falkewicz, A.; Faulkner, P.J.W.; Favart, L.; Fedotov, A.; Felst, R.; Feltesse, J.; Ferencei, J.; Finke, L.; Fleischer, M.; Flucke, G.; Fomenko, A.; Franke, G.; Frisson, T.; Gabathuler, E.; Garutti, E.; Gayler, J.; Gerlich, C.; Ghazaryan, Samvel; Ginzburgskaya, S.; Glazov, A.; Glushkov, I.; Goerlich, L.; Goettlich, M.; Gogitidze, N.; Gorbounov, S.; Grab, C.; Greenshaw, T.; Gregori, M.; Grell, B.R.; Grindhammer, G.; Gwilliam, C.; Habib, S.; Haidt, D.; Hansson, M.; Heinzelmann, G.; Henderson, R.C.W.; Henschel, H.; Herrera, G.; Hildebrandt, M.; Hiller, K.H.; Hoffmann, D.; Horisberger, R.; Hovhannisyan, A.; Hreus, T.; Hussain, S.; Ibbotson, M.; Ismail, M.; Jacquet, M.; Janssen, X.; Jemanov, V.; Jonsson, L.; Johnson, D.P.; Jung, Andreas Werner; Jung, H.; Kapichine, M.; Katzy, J.; Kenyon, I.R.; Kiesling, Christian M.; Klein, M.; Kleinwort, C.; Klimkovich, T.; Kluge, T.; Knies, G.; Knutsson, A.; Korbel, V.; Kostka, P.; Krastev, K.; Kretzschmar, J.; Kropivnitskaya, A.; Kruger, K.; Landon, M.P.J.; Lange, W.; Lastovicka-Medin, G.; Laycock, P.; Lebedev, A.; Leibenguth, G.; Lendermann, V.; Levonian, S.; Lindfeld, L.; Lipka, K.; Liptaj, A.; List, B.; List, J.; Lobodzinska, E.; Loktionova, N.; Lopez-Fernandez, R.; Lubimov, V.; Lucaci-Timoce, A.I.; Lueders, H.; Lux, T.; Lytkin, L.; Makankine, A.; Malden, N.; Malinovski, E.; Marage, P.; Marshall, R.; Marti, L.; Martisikova, M.; Martyn, H.U.; Maxfield, S.J.; Mehta, A.; Meier, K.; Meyer, A.B.; Meyer, H.; Meyer, J.; Michels, V.; Mikocki, S.; Milcewicz-Mika, I.; Milstead, D.; Mladenov, D.; Mohamed, A.; Moreau, F.; Morozov, A.; Morris, J.V.; Mozer, Matthias Ulrich; Muller, K.; Murin, P.; Nankov, K.; Naroska, B.; Naumann, T.; Newman, Paul R.; Niebuhr, C.; Nikiforov, A.; Nowak, G.; Nowak, K.; Nozicka, M.; Oganezov, R.; Olivier, B.; Olsson, J.E.; Osman, S.; Ozerov, D.; Palichik, V.; Panagoulias, I.; Papadopoulou, T.; Pascaud, C.; Patel, G.D.; Peng, H.; Perez, E.; Perez-Astudillo, D.; Perieanu, A.; Petrukhin, A.; Pitzl, D.; Placakyte, R.; Portheault, B.; Povh, B.; Prideaux, P.; Rahmat, A.J.; Raicevic, N.; Reimer, P.; Rimmer, A.; Risler, C.; Rizvi, E.; Robmann, P.; Roland, B.; Roosen, R.; Rostovtsev, A.; Rurikova, Z.; Rusakov, S.; Salvaire, F.; Sankey, D.P.C.; Sauter, M.; Sauvan, E.; Schmidt, S.; Schmitt, S.; Schmitz, C.; Schoeffel, L.; Schoning, A.; Schultz-Coulon, H.C.; Sefkow, F.; Shaw-West, R.N.; Sheviakov, I.; Shtarkov, L.N.; Sloan, T.; Smirnov, P.; Soloviev, Y.; South, D.; Spaskov, V.; Specka, Arnd E.; Steder, M.; Stella, B.; Stiewe, J.; Stoilov, A.; Straumann, U.; Sunar, D.; Tchoulakov, V.; Thompson, G.; Thompson, P.D.; Toll, T.; Tomasz, F.; Traynor, D.; Trinh, T.N.; Truoel, P.; Tsakov, I.; Tsipolitis, G.; Tsurin, I.; Turnau, J.; Tzamariudaki, E.; Urban, K.; Urban, Marcel; Usik, A.; Utkin, D.; Valkarova, A.; Vallee, C.; Van Mechelen, P.; Vargas Trevino, A.; Vazdik, Y.; Veelken, C.; Vinokurova, S.; Volchinski, V.; Wacker, K.; Weber, G.; Weber, R.; Wegener, D.; Werner, C.; Wessels, M.; Wessling, B.; Wissing, C.; Wolf, R.; Wunsch, E.; Xella, S.; Yan, W.; Yeganov, V.; Zacek, J.; Zalesak, J.; Zhang, Z.; Zhelezov, A.; Zhokin, A.; Zhu, Y.C.; Zimmermann, J.; Zimmermann, T.; Zohrabyan, H.; Zomer, F.

2007-01-01

Differential photoproduction cross sections are measured for events containing D* mesons. The data were taken with the H1 detector at the ep collider HERA and correspond to an integrated luminosity of 51.1 pb-1. The kinematic region covers small photon virtualities Q^2 < 0.01 GeV^2 and photon-proton centre-of-mass energies of 171 < W_gammap < 256 GeV. The details of the heavy quark production process are further investigated in events with one or two jets in addition to the D* meson. Differential cross sections for D* jet production are determined and the correlations between the D* meson and the jet(s) are studied. The results are compared with perturbative QCD predictions applying collinear- or kt -factorisation.

Multimodal nanoparticles as alignment and correlation markers in fluorescence/soft X-ray cryo-microscopy/tomography of nucleoplasmic reticulum and apoptosis in mammalian cells

International Nuclear Information System (INIS)

Hagen, Christoph; Werner, Stephan; Carregal-Romero, Susana; Malhas, Ashraf N.; Klupp, Barbara G.; Guttmann, Peter; Rehbein, Stefan; Henzler, Katja; Mettenleiter, Thomas C.

2014-01-01

Correlative fluorescence and soft X-ray cryo-microscopy/tomography on flat sample holders is perfectly suited to study the uncompromised physiological status of adherent cells at its best possible preservation by imaging after fast cryo-immobilization. To understand the mechanism by which herpesviruses induce nucleoplasmic reticulum, i.e. invaginations of the nuclear envelope, during their egress from the host cell nucleus, morphologically similar structures found in laminopathies and after chemical induction were investigated as a potentially more easily accessible model system. For example, anti-retroviral protease inhibitors like Saquinavir also induce invaginations of the nuclear membranes. With the help of newly designed multimodal nanoparticles as alignment and correlation markers, and by optimizing fluorescence cryo-microscopy data acquisition, an elaborate three-dimensional network of nucleoplasmic reticulum was demonstrated in nuclei of Saquinavir-treated rabbit kidney cells expressing a fluorescently labeled inner nuclear membrane protein. In part of the protease inhibitor-treated samples, nuclei exhibited dramatic ultrastructural changes indicative of programmed cell death/apoptosis. This unexpected observation highlights another unique feature of soft X-ray microscopy, i.e. high absorption contrast information not relying on labeled cellular components, at a 3D resolution of approximately 40 nm (half-pitch) and through a sample thickness of several micrometers. These properties make it a valuable part of the cell biology imaging toolbox to visualize the cellular ultrastructure in its completeness. - Highlights: • Nucleoplasmic reticulum was demonstrated in nuclei of Saquinavir-treated cells. • New polyelectrolyte-Qdot ® 605 coated gold beads were employed as fiducials. • Saquinavir can induce a strong apoptotic phenotype in the nucleus. • CryoXT is an auspicious imaging technique in apoptosis research

Effects of signal modulation and coloured cross-correlation of coloured noises on the diffusion of a harmonic oscillator

Institute of Scientific and Technical Information of China (English)

Liu Li; Zhang Liang-Ying; Cao Li

2009-01-01

The diffusion in a harmonic oscillator driven by coloured noises ζ(t) and η(t) with coloured cross-correlation in which one of the noises is modulated by a biased periodic signal is investigated. The exact expression of diffusion coefficient d as a function of noise parameter, signal parameter, and oscillator frequency is derived. The findings in this paper are as follows. 1) The curves of d versus noise intensity D and d versus noises cross-correlation time τ_3 exist as two different phases. The transition between the two phases arises from the change of the cross-correlation coefficient λ of the two Orustein-Uhlenbeck (O-U) noises. 2) Changing the value of τ3, the curves of d versus Q, the intensity of colored noise that is modulated by the signal, can transform from a phase having a minimum to a monotonic phase. 3)Changing the value of signal amplitude A, d versus Q curves can transform from a phase having a minimum to a monotonic phase. The above-mentioned results demonstrate that a like noise-induced transition appears in the model.

Effects of signal modulation and coloured cross-correlation of coloured noises on the diffusion of a harmonic oscillator

International Nuclear Information System (INIS)

Li, Liu; Li, Cao; Liang-Ying, Zhang

2009-01-01

The diffusion in a harmonic oscillator driven by coloured noises ζ(t) and η(t) with coloured cross-correlation in which one of the noises is modulated by a biased periodic signal is investigated. The exact expression of diffusion coefficient d as a function of noise parameter, signal parameter, and oscillator frequency is derived. The findings in this paper are as follows. 1) The curves of d versus noise intensity D and d versus noises cross-correlation time τ 3 exist as two different phases. The transition between the two phases arises from the change of the cross-correlation coefficient λ of the two Ornstein–Uhlenbeck (O-U) noises. 2) Changing the value of τ 3 , the curves of d versus Q, the intensity of colored noise that is modulated by the signal, can transform from a phase having a minimum to a monotonic phase. 3) Changing the value of signal amplitude A, d versus Q curves can transform from a phase having a minimum to a monotonic phase. The above-mentioned results demonstrate that a like noise-induced transition appears in the model. (general)

Photo-triggered fluorescent theranostic prodrugs as DNA alkylating agents for mechlorethamine release and spatiotemporal monitoring.

Science.gov (United States)

Cao, Yanting; Pan, Rong; Xuan, Weimin; Wei, Yongyi; Liu, Kejian; Zhou, Jiahong; Wang, Wei

2015-06-28

We describe a new theranostic strategy for selective delivery and spatiotemporal monitoring of mechlorethamine, a DNA alkylating agent. A photo-responsive prodrug is designed and composed of a photolabile o-nitrophenylethyl group, a DNA alkylating mechlorethamine drug and a coumarin fluorophore. Masking of the "N" in mechlorethamine in a positively charged state in the prodrug renders it inactive, non-toxic, selective and non-fluorescent. Indeed, the stable prodrug shows negligible cytotoxicity towards normal cells with and without UV activation and is completely non-fluorescent. However, upon photo-irradiation, the active mechlorethamine is released and induces efficient DNA cross-links, accompanied by a strong fluorescence enhancement (152 fold). Furthermore, DNA cross-linking activity from the release can be transformed into anticancer activity observed in in vitro studies of tumor cells. Importantly, the drug release progress and the movement can be conveniently monitored by fluorescence spectroscopy. The mechanistic study proves that the DNA cross-linking activity is mainly due to the release of DNA alkylating mechlorethamine. Altogether, the studies show the power of the theranostic strategy for efficient therapy in cancer treatment.

Seismic reflection response from cross-correlations of ambient vibrations on non-conventional hidrocarbon reservoir

Science.gov (United States)

Huerta, F. V.; Granados, I.; Aguirre, J.; Carrera, R. Á.

2017-12-01

Nowadays, in hydrocarbon industry, there is a need to optimize and reduce exploration costs in the different types of reservoirs, motivating the community specialized in the search and development of alternative exploration geophysical methods. This study show the reflection response obtained from a shale gas / oil deposit through the method of seismic interferometry of ambient vibrations in combination with Wavelet analysis and conventional seismic reflection techniques (CMP & NMO). The method is to generate seismic responses from virtual sources through the process of cross-correlation of records of Ambient Seismic Vibrations (ASV), collected in different receivers. The seismic response obtained is interpreted as the response that would be measured in one of the receivers considering a virtual source in the other. The acquisition of ASV records was performed in northern of Mexico through semi-rectangular arrays of multi-component geophones with instrumental response of 10 Hz. The in-line distance between geophones was 40 m while in cross-line was 280 m, the sampling used during the data collection was 2 ms and the total duration of the records was 6 hours. The results show the reflection response of two lines in the in-line direction and two in the cross-line direction for which the continuity of coherent events have been identified and interpreted as reflectors. There is certainty that the events identified correspond to reflections because the time-frequency analysis performed with the Wavelet Transform has allowed to identify the frequency band in which there are body waves. On the other hand, the CMP and NMO techniques have allowed to emphasize and correct the reflection response obtained during the correlation processes in the frequency band of interest. The results of the processing and analysis of ASV records through the seismic interferometry method have allowed us to see interesting results in light of the cross-correlation process in combination with

Intensive care antibiotic consumption and resistance patterns: a cross-correlation analysis

Directory of Open Access Journals (Sweden)

Luminita Baditoiu

2017-11-01

Full Text Available Abstract Background Over recent decades, a dramatic increase in infections caused by multidrug-resistant pathogens has been observed worldwide. The aim of the present study was to investigate the relationship between local resistance bacterial patterns and antibiotic consumption in an intensive care unit in a Romanian university hospital. Methods A prospective study was conducted between 1st January 2012 and 31st December 2013. Data covering the consumption of antibacterial drugs and the incidence density for the main resistance phenotypes was collected on a monthly basis, and this data was aggregated quarterly. The relationship between the antibiotic consumption and resistance was investigated using cross-correlation, and four regression models were constructed, using the SPSS version 20.0 (IBM, Chicago, IL and the R version 3.2.3 packages. Results During the period studied, the incidence of combined-resistant and carbapenem-resistant P. aeruginosa strains increased significantly [(gradient = 0.78, R2 = 0.707, p = 0.009 (gradient = 0.74, R2 = 0.666, p = 0.013 respectively], mirroring the increase in consumption of β-lactam antibiotics with β-lactamase inhibitors (piperacillin/tazobactam and carbapenems (meropenem [(gradient = 10.91, R2 = 0.698, p = 0.010 and (gradient = 14.63, R2 = 0.753, p = 0.005 respectively]. The highest cross-correlation coefficients for zero time lags were found between combined-resistant vs. penicillins consumption and carbapenem-resistant P. aeruginosa strains vs. carbapenems consumption (0.876 and 0.928, respectively. The best model describing the relation between combined-resistant P. aeruginosa strains and penicillins consumption during a given quarter incorporates both the consumption and the incidence of combined-resistant strains in the hospital department during the previous quarter (multiple R2 = 0.953, p = 0.017. The best model for explaining the carbapenem resistance of P

High precision Cross-correlated imaging in Few-mode fibers

DEFF Research Database (Denmark)

Muliar, Olena; Usuga Castaneda, Mario A.; Kristensen, Torben

2017-01-01

us to distinguishing differential time delays between HOMs in the picosecond timescale. Broad wavelength scanning in combination with spectral shaping, allows us to estimate the modal behavior of FMF without prior knowledge of the fiber parameters. We performed our demonstration at wavelengths from...... existing approaches for modal content analysis, several methods as S2, C2 in time and frequency domain are available. In this contribution we will present an improved time-domain cross-correlated (C2) imaging technique for the experimental evaluation of modal properties in HOM fibers over a broad range......) in a few-mode fiber (FMF) are used as multiple spatial communication channels, comes in this context as a viable approach to enable the optimization of high-capacity links. From this perspective, it becomes highly necessary to possess a diagnostic tool for the precise modal characterization of FMFs. Among...

Measurement of L X-ray fluorescence cross-sections for elements with 45 ≤ Z ≤ 50 using synchrotron radiation at 8 keV

International Nuclear Information System (INIS)

Bonzi, Edgardo V.; Badiger, Nagappa M.; Grad, Gabriela B.; Barrea, Raul A.; Figueroa, Rodolfo G.

2011-01-01

The L shell fluorescence cross-sections of the elements in range 45 ≤ Z ≤ 50 have been determined at 8 keV using Synchrotron radiation. The individual L X-ray photons, Ll, Lα, Lβ I , Lβ II , Lγ I and Lγ II produced in the target were measured with high resolution Si(Li) detector. The experimental set-up provided a low background by using linearly polarized monoenergetic photon beam, improving the signal-to-noise ratio. The experimental cross-sections obtained in this work were compared with available experimental data from Scofield Krause and Scofield and Puri et al. . These experimental values closely agree with the theoretical values calculated using Scofield and Krause data, except for the case of Lγ, where values measured of this work are slighter higher.

Diaminobenzidine photoconversion is a suitable tool for tracking the intracellular location of fluorescently labelled nanoparticles at transmission electron microscopy

Directory of Open Access Journals (Sweden)

M. Malatesta

2012-04-01

Full Text Available Chitosan-based nanoparticles (NPs deserve particular attention as suitable drug carriers in the field of pharmaceutics, since they are able to protect the encapsulated drugs and/or improve their efficacy by making them able to cross biological barriers (such as the blood-brain barrier and reach their intracellular target sites. Understanding the intracellular location of NPs is crucial for designing drug delivery strategies. In this study, fluorescently-labelled chitosan NPs were administered in vitro to a neuronal cell line, and diaminobenzidine (DAB photoconversion was applied to correlate fluorescence and transmission electron microscopy to precisely describe the NPs intracellular fate. This technique allowed to demonstrate that chitosan NPs easily enter neuronal cells, predominantly by endocytosis; they were found both inside membrane-bounded vesicles and free in the cytosol, and were observed to accumulate around the cell nucleus.

Multifractal Cross Wavelet Analysis

Science.gov (United States)

Jiang, Zhi-Qiang; Gao, Xing-Lu; Zhou, Wei-Xing; Stanley, H. Eugene

Complex systems are composed of mutually interacting components and the output values of these components usually exhibit long-range cross-correlations. Using wavelet analysis, we propose a method of characterizing the joint multifractal nature of these long-range cross correlations, a method we call multifractal cross wavelet analysis (MFXWT). We assess the performance of the MFXWT method by performing extensive numerical experiments on the dual binomial measures with multifractal cross correlations and the bivariate fractional Brownian motions (bFBMs) with monofractal cross correlations. For binomial multifractal measures, we find the empirical joint multifractality of MFXWT to be in approximate agreement with the theoretical formula. For bFBMs, MFXWT may provide spurious multifractality because of the wide spanning range of the multifractal spectrum. We also apply the MFXWT method to stock market indices, and in pairs of index returns and volatilities we find an intriguing joint multifractal behavior. The tests on surrogate series also reveal that the cross correlation behavior, particularly the cross correlation with zero lag, is the main origin of cross multifractality.

Study on fluorescence of Maillard reaction compounds in breakfast cereals.

Science.gov (United States)

Delgado-Andrade, Cristina; Rufián-Henares, José A; Morales, Francisco J

2006-09-01

During the advanced stage of the Maillard reaction (MR) in food processing and cooking, Amadori rearrangement products undergo dehydration and fission and fluorescent substances are formed. Free and total (free + linked to the protein backbone) fluorescence (FIC) due to Maillard compounds in 60 commercial breakfast cereals was evaluated. Pronase was used for efficient release of linked fluorescent Maillard compounds from the protein backbone. Results were correlated with some heat-induced markers of the extent of the MR or sugar caramelisation during cereal processing, such as hydroxymethylfurfural, furfural, glucosilisomaltol and furosine. The effect of sample composition (dietary-fibre added, protein, etc.) on levels of FIC, expressed as fluorescence intensity (FI) per milligram of sample, is discussed. FIC is significantly correlated to the protein content of the sample and fluorescent Maillard compounds are mainly linked to the protein backbone. The ratio of total-FIC to free-FIC was 10.4-fold for corn-based, wheat-based and multicereal-based breakfast cereals but significantly higher in rice-based samples. Addition of dietary fibre or honey increased the FIC values. Data support the usefulness of FIC measurement as an unspecific heat-induced marker in breakfast cereals.

Interdependence between crude oil and world food prices: A detrended cross correlation analysis

Science.gov (United States)

Pal, Debdatta; Mitra, Subrata K.

2018-02-01

This article explores the changing interdependence between crude oil and world food prices at varying time scales using detrended cross correlation analysis that would answer whether the interdependence (if any) differed significantly between pre and post-crisis period. Unlike the previous studies that exogenously imposed break dates for dividing the time series into sub-samples, we tested whether the mean of the crude oil price changed over time to find evidence for structural changes in the crude oil price series and endogenously determine three break dates with minimum Bayesian information criterion scores. Accordingly, we divided the entire study period in four sample periods - January 1990 to October 1999, November 1999 to February 2005, March 2005 to September 2010, and October 2010 to July 2016, where the third sample period coincided with the period of food crisis and enabled us to compare the fuel-food interdependence across pre-crisis, during the crisis, and post-crisis periods. The results of the detrended cross correlation analysis extended corroborative evidence for increasing positive interdependence between the crude oil price and world food price index along with its sub-categories, namely dairy, cereals, vegetable oil, and sugar. The article ends with the implications of these results in the domain of food policy and the financial sector.

Cross-correlation analysis of pulse wave propagation in arteries: in vitro validation and in vivo feasibility.

Science.gov (United States)

Nauleau, Pierre; Apostolakis, Iason; McGarry, Matthew; Konofagou, Elisa

2018-05-29

The stiffness of the arteries is known to be an indicator of the progression of various cardiovascular diseases. Clinically, the pulse wave velocity (PWV) is used as a surrogate for arterial stiffness. Pulse wave imaging (PWI) is a non-invasive, ultrasound-based imaging technique capable of mapping the motion of the vessel walls, allowing the local assessment of arterial properties. Conventionally, a distinctive feature of the displacement wave (e.g. the 50% upstroke) is tracked across the map to estimate the PWV. However, the presence of reflections, such as those generated at the carotid bifurcation, can bias the PWV estimation. In this paper, we propose a two-step cross-correlation based method to characterize arteries using the information available in the PWI spatio-temporal map. First, the area under the cross-correlation curve is proposed as an index for locating the regions of different properties. Second, a local peak of the cross-correlation function is tracked to obtain a less biased estimate of the PWV. Three series of experiments were conducted in phantoms to evaluate the capabilities of the proposed method compared with the conventional method. In the ideal case of a homogeneous phantom, the two methods performed similarly and correctly estimated the PWV. In the presence of reflections, the proposed method provided a more accurate estimate than conventional processing: e.g. for the soft phantom, biases of  -0.27 and -0.71 m · s -1 were observed. In a third series of experiments, the correlation-based method was able to locate two regions of different properties with an error smaller than 1 mm. It also provided more accurate PWV estimates than conventional processing (biases:  -0.12 versus -0.26 m · s -1 ). Finally, the in vivo feasibility of the proposed method was demonstrated in eleven healthy subjects. The results indicate that the correlation-based method might be less precise in vivo but more accurate than the conventional method.

Porphyrin involvement in redshift fluorescence in dentin decay

Science.gov (United States)

Slimani, A.; Panayotov, I.; Levallois, B.; Cloitre, T.; Gergely, C.; Bec, N.; Larroque, C.; Tassery, H.; Cuisinier, F.

2014-05-01

The aim of this study was to evaluate the porphyrin involvement in the red fluorescence observed in dental caries with Soprolife® light-induced fluorescence camera in treatments mode (SOPRO, ACTEON Group, La Ciotat, France) and Vistacam® camera (DÜRR DENTAL AG, Bietigheim-Bissingen, Germany). The International Caries Detection and Assessment System (ICDAS) was used to rand the samples. Human teeth cross-sections, ranked from ICDAS score 0 to 6, were examined by epi-fluorescence microscopy and Confocal Raman microscopy. Comparable studies were done with Protoporphyrin IX, Porphyrin I and Pentosidine solutions. An RGB analysis of Soprolife® images was performed using ImageJ Software (1.46r, National Institutes of Health, USA). Fluorescence spectroscopy and MicroRaman spectroscopy revealed the presence of Protoporphyrin IX, in carious enamel, dentin and dental plaque. However, the presence of porphyrin I and pentosidine cannot be excluded. The results indicated that not only porphyrin were implicated in the red fluorescence, Advanced Glygation Endproducts (AGEs) of the Maillard reaction also contributed to this phenomenon.

Optical imaging of non-fluorescent nanoparticle probes in live cells

Energy Technology Data Exchange (ETDEWEB)

Wang, Gufeng; Stender, Anthony S.; Sun, Wei; and Fang, Ning

2009-12-17

Precise imaging of cellular and subcellular structures and dynamic processes in live cells is crucial for fundamental research in life sciences and in medical applications. Non-fluorescent nanoparticles are an important type of optical probe used in live-cell imaging due to their photostability, large optical cross-sections, and low toxicity. Here, we provide an overview of recent developments in the optical imaging of non-fluorescent nanoparticle probes in live cells.

Cross-correlating Cosmic IR and X-ray Background Fluctuations: Evidence of Significant Black Hole Populations Among the CIB Sources

Science.gov (United States)

Cappelluti, N.; Kashlinsky, A.; Arendt, R. G.; Comastri, A.; Fazio, G. G.; Finoguenov, A.; Hasinger, G.; Mather, J. C.; Miyaji, T; Moseley, S. H.

2013-01-01

In order to understand the nature of the sources producing the recently uncovered cosmic infrared background (CIB) fluctuations, we study cross-correlations between the fluctuations in the source-subtracted CIB from Spitzer/IRAC data and the unresolved cosmic X-ray background from deep Chandra observations. Our study uses data from the EGS/AEGIS field, where both data sets cover an approx = 8' x 45' region of the sky. Our measurement is the cross-power spectrum between the IR and X-ray data. The cross-power signal between the IRAC maps at 3.6 micron and 4.5 micron and the Chandra [0.5-2] keV data has been detected, at angular scales approx >20'', with an overall significance of approx = 3.8 sigma and approx. = 5.6 sigma, respectively. At the same time we find no evidence of significant cross-correlations at the harder Chandra bands. The cross-correlation signal is produced by individual IR sources with 3.6 micron and 4.5 micron magnitudes m(sub AB) approx. > 25-26 and [0.5-2] keV X-ray fluxes black holes than among the known populations. We discuss the various possible origins for the cross-power signal and show that neither local foregrounds nor the known remaining normal galaxies and active galactic nuclei can reproduce the measurements. These observational results are an important new constraint on theoretical modeling of the near-IR CIB fluctuations. local foregrounds, nor the known remaining normal galaxies and active galactic nuclei (AGN) can reproduce the measurements. These observational results are an important new constraint on theoretical modeling of the near-IR CIB fluctuations

Study of x-ray fluorescence : Development in Geant4 of new models of cross sections for simulation PIXE. Biological and archaeological applications

International Nuclear Information System (INIS)

Ben Abdelouahed, Haifa

2010-01-01

a great number of times, that makes it possible to reproduce the macroscopic effects and to calculate sizes like, in our case, x-ray fluorescence. Among several tools available for Monte Carlo simulation of the interactions particle-matter, the tool for simulation Monte Carlo Geant4 is characterized by a particularly flexible architecture based on directed technology object. This is why, to build our code of simulation, our choice was fixed on the use of this tool. We treat in the first chapter the guiding principle of x-ray fluorescence, which leads us to present the various phenomena which limit its sensitivity of analysis. We devote the second chapter to the presentation of the Geant4 tool, and our tests of validation of its electromagnetic processes. We describe the performances and the limits of this tool as for the simulation of the cross sections. We will expose in this same chapter our development, in the Geant4 tool, of new models of calculation of the cross sections of ionization of the atoms by the protons and the particles alpha. We discuss also our method of validation of the models in question. This work was object of publication. It was taken into account by the Geant4 project: our development currently replaces the data base EEDL of Geant4 in the process of determination of the cross sections of ionization by charged particles, and makes thus functional the Geant4 tool in the simulation of the x-ray fluorescence induced by charged particles. We describe in the third chapter our use of the code worked out for the simulation of the effectiveness of absorption of the standard Si(Li) detector which constitutes the essential piece of our device of analysis by x-ray fluorescence. We expose the adopted approach which enabled us to optimize the geometrical parameters of the studied detector. This work was presented at the second international conference of spectroscopy. We present, in the fourth chapter, some examples of concrete applications on the study of

Measurement of protein-like fluorescence in river and waste water using a handheld spectrophotometer.

Science.gov (United States)

Baker, Andy; Ward, David; Lieten, Shakti H; Periera, Ryan; Simpson, Ellie C; Slater, Malcolm

2004-07-01

Protein-like fluorescence intensity in rivers increases with increasing anthropogenic DOM inputs from sewerage and farm wastes. Here, a portable luminescence spectrophotometer was used to investigate if this technology could be used to provide both field scientists with a rapid pollution monitoring tool and process control engineers with a portable waste water monitoring device, through the measurement of river and waste water tryptophan-like fluorescence from a range of rivers in NE England and from effluents from within two waste water treatment plants. The portable spectrophotometer determined that waste waters and sewerage effluents had the highest tryptophan-like fluorescence intensity, urban streams had an intermediate tryptophan-like fluorescence intensity, and the upstream river samples of good water quality the lowest tryptophan-like fluorescence intensity. Replicate samples demonstrated that fluorescence intensity is reproducible to +/- 20% for low fluorescence, 'clean' river water samples and +/- 5% for urban water and waste waters. Correlations between fluorescence measured by the portable spectrophotometer with a conventional bench machine were 0.91; (Spearman's rho, n = 143), demonstrating that the portable spectrophotometer does correlate with tryptophan-like fluorescence intensity measured using the bench spectrophotometer.

Nonlinear stochastic exclusion financial dynamics modeling and time-dependent intrinsic detrended cross-correlation

Science.gov (United States)

Zhang, Wei; Wang, Jun

2017-09-01

In attempt to reproduce price dynamics of financial markets, a stochastic agent-based financial price model is proposed and investigated by stochastic exclusion process. The exclusion process, one of interacting particle systems, is usually thought of as modeling particle motion (with the conserved number of particles) in a continuous time Markov process. In this work, the process is utilized to imitate the trading interactions among the investing agents, in order to explain some stylized facts found in financial time series dynamics. To better understand the correlation behaviors of the proposed model, a new time-dependent intrinsic detrended cross-correlation (TDI-DCC) is introduced and performed, also, the autocorrelation analyses are applied in the empirical research. Furthermore, to verify the rationality of the financial price model, the actual return series are also considered to be comparatively studied with the simulation ones. The comparison results of return behaviors reveal that this financial price dynamics model can reproduce some correlation features of actual stock markets.

The Atacama Cosmology Telescope: Cross-Correlation of Cosmic Microwave Background Lensing and Quasars

Science.gov (United States)

Sherwin, Blake D; Das, Sudeep; Haijian, Amir; Addison, Graeme; Bond, Richard; Crichton, Devin; Devlin, Mark J.; Dunkley, Joanna; Gralla, Megan B.; Halpern, Mark;

Fluorescence detection of esophageal neoplasia

Science.gov (United States)

Borisova, E.; Vladimirov, B.; Avramov, L.

2008-06-01

White-light endoscopy is well-established and wide used modality. However, despite the many technological advances that have been occurred, conventional endoscopy is suboptimal and usually detects advanced stage lesions. The limitations of standard endoscopy initiate development of spectroscopic techniques, additional to standard endoscopic equipment. One of the most sensitive approaches is fluorescence spectroscopy of gastrointestinal mucosa for neoplasia detection. In the recent study delta-aminolevulinic acid/Protoporphyrin IX (5-ALA/PpIX) is used as fluorescent marker for dysplasia and tumor detection in esophagus. The 5-ALA is administered per os six hours before measurements at dose 20 mg/kg weight. Excitation source has max of emission at 405 nm and light is delivered by the standard light guide of the endoscopic equipment. Through endoscopic instrumental channel a fiber is applied to return information about fluorescence to microspectrometer. Spectral features observed during endoscopic investigations could be distinct as the next regions: 450-630 nm region, where tissue autofluorescence is observed; 630-710 nm region, where fluorescence of PpIX is clearly pronounced; 530-580 nm region, where minima in the autofluorescence signal are observed, related to reabsorption of blood. The lack of fluorescence peaks in the red spectral area for normal mucosa is an indication for selective accumulation of 5-ALA/PpIX only in abnormal sites Very good correlation between fluorescence signals and histology examination of the lesions investigated is achieved.

Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell

International Nuclear Information System (INIS)

Ji Dongmei; Lv Wei; Huang Zhengxi; Xia Andong; Xu Min; Ma Weimin; Mi Hualing; Ogawa Teruo

2007-01-01

The laser confocal scanning microscopy combined with time-correlated single photon counting imaging technique to obtain fluorescence intensity and fluorescence lifetime images for fluorescence resonance energy transfer measurement is reported. Both the fluorescence lifetime imaging microscopy (FLIM) and intensity images show inhomogeneous cyan fluorescent protein and yellow fluorescent protein (CFP /YFP) expression or inhomogeneous energy transfer between CFP and YFP over whole cell. The results presented in this work show that FLIM could be a potential method to reveal the structure-function behavior of NAD(P)H dehydrogenase complexes in living cell

Detection of non-stationary leak signals at NPP primary circuit by cross-correlation analysis

International Nuclear Information System (INIS)

Shimanskij, S.B.

2007-01-01

A leak-detection system employing high-temperature microphones has been developed for the RBMK and ATR (Japan) reactors. Further improvement of the system focused on using cross-correlation analysis of the spectral components of the signal to detect a small leak at an early stage of development. Since envelope processes are less affected by distortions than are wave processes, they give a higher-degree of correlation and can be used to detect leaks with lower signal-noise ratios. Many simulation tests performed at nuclear power plants have shown that the proposed methods can be used to detect and find the location of a small leak [ru

Experimental determination of L X-ray fluorescence cross sections for elements with 45 < Z < 50 at 10 keV

Directory of Open Access Journals (Sweden)

Edgardo Bonzi

2014-02-01

Full Text Available Synchrotron radiation at 10 keV was used to experimentally determine the Ll, L, LI, LII, LI and LII fluorescence cross sections for elements with 45 < Z < 50, as part of an ongoing investigation at low energies. The measured data were compared with calculated values obtained using coefficients from Scofield, Krause and Puri et al. Received: 19 December 2013, Accepted: 20 January 2014; Reviewed by: J. P. Marques, Departamento de Física, Centro de Física Atómica, Fac. de Ciências, Universidade de Lisboa, Portugal.; Edited by: P. Weck; DOI: http://dx.doi.org/10.4279/PIP.060001 Cite as: E V Bonzi, G B Grad, R A Barrea, Papers in Physics 6, 060001 (2014

Pleasant/Unpleasant Filtering for Affective Image Retrieval Based on Cross-Correlation of EEG Features

Directory of Open Access Journals (Sweden)

Keranmu Xielifuguli

2014-01-01

Full Text Available People often make decisions based on sensitivity rather than rationality. In the field of biological information processing, methods are available for analyzing biological information directly based on electroencephalogram: EEG to determine the pleasant/unpleasant reactions of users. In this study, we propose a sensitivity filtering technique for discriminating preferences (pleasant/unpleasant for images using a sensitivity image filtering system based on EEG. Using a set of images retrieved by similarity retrieval, we perform the sensitivity-based pleasant/unpleasant classification of images based on the affective features extracted from images with the maximum entropy method: MEM. In the present study, the affective features comprised cross-correlation features obtained from EEGs produced when an individual observed an image. However, it is difficult to measure the EEG when a subject visualizes an unknown image. Thus, we propose a solution where a linear regression method based on canonical correlation is used to estimate the cross-correlation features from image features. Experiments were conducted to evaluate the validity of sensitivity filtering compared with image similarity retrieval methods based on image features. We found that sensitivity filtering using color correlograms was suitable for the classification of preferred images, while sensitivity filtering using local binary patterns was suitable for the classification of unpleasant images. Moreover, sensitivity filtering using local binary patterns for unpleasant images had a 90% success rate. Thus, we conclude that the proposed method is efficient for filtering unpleasant images.

Multifractality, efficiency analysis of Chinese stock market and its cross-correlation with WTI crude oil price

Science.gov (United States)

Zhuang, Xiaoyang; Wei, Yu; Ma, Feng

2015-07-01

In this paper, the multifractality and efficiency degrees of ten important Chinese sectoral indices are evaluated using the methods of MF-DFA and generalized Hurst exponents. The study also scrutinizes the dynamics of the efficiency of Chinese sectoral stock market by the rolling window approach. The overall empirical findings revealed that all the sectoral indices of Chinese stock market exist different degrees of multifractality. The results of different efficiency measures have agreed on that the 300 Materials index is the least efficient index. However, they have a slight diffidence on the most efficient one. The 300 Information Technology, 300 Telecommunication Services and 300 Health Care indices are comparatively efficient. We also investigate the cross-correlations between the ten sectoral indices and WTI crude oil price based on Multifractal Detrended Cross-correlation Analysis. At last, some relevant discussions and implications of the empirical results are presented.

Comparison of nanoparticle diffusion using fluorescence correlation spectroscopy and differential dynamic microscopy within concentrated polymer solutions

Science.gov (United States)

Shokeen, Namita; Issa, Christopher; Mukhopadhyay, Ashis

2017-12-01

We studied the diffusion of nanoparticles (NPs) within aqueous entangled solutions of polyethylene oxide (PEO) by using two different optical techniques. Fluorescence correlation spectroscopy, a method widely used to investigate nanoparticle dynamics in polymer solution, was used to measure the long-time diffusion coefficient (D) of 25 nm radius particles within high molecular weight, Mw = 600 kg/mol PEO in water solutions. Differential dynamic microscopy (DDM) was used to determine the wave-vector dependent dynamics of NPs within the same polymer solutions. Our results showed good agreement between the two methods, including demonstration of normal diffusion and almost identical diffusion coefficients obtained by both techniques. The research extends the scope of DDM to study the dynamics and rheological properties of soft matter at a nanoscale. The measured diffusion coefficients followed a scaling theory, which can be explained by the coupling between polymer dynamics and NP motion.

Automated detection of fluorescent cells in in-resin fluorescence sections for integrated light and electron microscopy.

Science.gov (United States)

Delpiano, J; Pizarro, L; Peddie, C J; Jones, M L; Griffin, L D; Collinson, L M

2018-04-26

Integrated array tomography combines fluorescence and electron imaging of ultrathin sections in one microscope, and enables accurate high-resolution correlation of fluorescent proteins to cell organelles and membranes. Large numbers of serial sections can be imaged sequentially to produce aligned volumes from both imaging modalities, thus producing enormous amounts of data that must be handled and processed using novel techniques. Here, we present a scheme for automated detection of fluorescent cells within thin resin sections, which could then be used to drive automated electron image acquisition from target regions via 'smart tracking'. The aim of this work is to aid in optimization of the data acquisition process through automation, freeing the operator to work on other tasks and speeding up the process, while reducing data rates by only acquiring images from regions of interest. This new method is shown to be robust against noise and able to deal with regions of low fluorescence. © 2018 The Authors. Journal of Microscopy published by JohnWiley & Sons Ltd on behalf of Royal Microscopical Society.

Kalman/Map Filtering-Aided Fast Normalized Cross Correlation-Based Wi-Fi Fingerprinting Location Sensing

Directory of Open Access Journals (Sweden)

Yongliang Sun

2013-11-01

Full Text Available A Kalman/map filtering (KMF-aided fast normalized cross correlation (FNCC-based Wi-Fi fingerprinting location sensing system is proposed in this paper. Compared with conventional neighbor selection algorithms that calculate localization results with received signal strength (RSS mean samples, the proposed FNCC algorithm makes use of all the on-line RSS samples and reference point RSS variations to achieve higher fingerprinting accuracy. The FNCC computes efficiently while maintaining the same accuracy as the basic normalized cross correlation. Additionally, a KMF is also proposed to process fingerprinting localization results. It employs a new map matching algorithm to nonlinearize the linear location prediction process of Kalman filtering (KF that takes advantage of spatial proximities of consecutive localization results. With a calibration model integrated into an indoor map, the map matching algorithm corrects unreasonable prediction locations of the KF according to the building interior structure. Thus, more accurate prediction locations are obtained. Using these locations, the KMF considerably improves fingerprinting algorithm performance. Experimental results demonstrate that the FNCC algorithm with reduced computational complexity outperforms other neighbor selection algorithms and the KMF effectively improves location sensing accuracy by using indoor map information and spatial proximities of consecutive localization results.

Kalman/Map filtering-aided fast normalized cross correlation-based Wi-Fi fingerprinting location sensing.

Science.gov (United States)

Sun, Yongliang; Xu, Yubin; Li, Cheng; Ma, Lin

2013-11-13

A Kalman/map filtering (KMF)-aided fast normalized cross correlation (FNCC)-based Wi-Fi fingerprinting location sensing system is proposed in this paper. Compared with conventional neighbor selection algorithms that calculate localization results with received signal strength (RSS) mean samples, the proposed FNCC algorithm makes use of all the on-line RSS samples and reference point RSS variations to achieve higher fingerprinting accuracy. The FNCC computes efficiently while maintaining the same accuracy as the basic normalized cross correlation. Additionally, a KMF is also proposed to process fingerprinting localization results. It employs a new map matching algorithm to nonlinearize the linear location prediction process of Kalman filtering (KF) that takes advantage of spatial proximities of consecutive localization results. With a calibration model integrated into an indoor map, the map matching algorithm corrects unreasonable prediction locations of the KF according to the building interior structure. Thus, more accurate prediction locations are obtained. Using these locations, the KMF considerably improves fingerprinting algorithm performance. Experimental results demonstrate that the FNCC algorithm with reduced computational complexity outperforms other neighbor selection algorithms and the KMF effectively improves location sensing accuracy by using indoor map information and spatial proximities of consecutive localization results.

Detection of occlusal caries lesions using fluorescence: correlation between histology and obtained results for Diagnodent and spectroscopy

International Nuclear Information System (INIS)

Rocha-Cabral, Renata Maciel

2006-01-01

The aims of this study were to develop and test a method to detect caries lesions in vivo and in vitro, using a portable spectrometer (PS); to analyze the performance of PS as well as the commercial device Diagnodent (Dd); correlate them with the gold standard, their transversal section areas and lesions depth and between themselves. 66 occlusal pre-molars sites were examined in vivo with Dd. Sequentially, fluorescence (λexc ∼ 657 nm) was collected by an optical fiber, conducted to PS and then analyzed as spectra, which were normalized and had calculated the Ratios of their Areas Under the Curves (RAUC) of carious and sound tissues. Experiments were conducted in vitro in the same sites. Gold Standard was obtained by polarized light microscopy. Pearson correlation was used to compare the devices with transversal section area, lesions depth and between themselves. The area under ROC curve, sensitivity, specificity as well as accuracy were calculated and verified with McNemar test. Dd and RAUC showed statistically significant correlation with gold standard (p < 0.01 for Dd and p < 0.05 for RAUC) and between themselves (r = 0,83 in vivo and r = 0,87 in vitro). Although it was significant, the devices showed low correlation with depth of lesions in vivo and in vitro (r = ∼ 0.43). The transversal section area of the lesion had no influence on readings in both devices. Dd showed higher sensitivity (0.76) than PS (0.60) in vivo (p < 0.05), though this fact was not able to improve its performance. In turn, PS showed higher sensitivity (0.88) than Dd (0.79) in vitro, but this difference was not significantly. The other parameters did not show statistically significant differences (p < 0.05) between methods. PS showed positive correlation with Dd, equal correlation with lesions depth and higher ability of detecting the disease in vitro, what suggests that if accompanied with a conic and an angulated probe and a dedicated software, the PS method could be useful in clinics

PROBING THE EPOCH OF PRE-REIONIZATION BY CROSS-CORRELATING COSMIC MICROWAVE AND INFRARED BACKGROUND ANISOTROPIES

International Nuclear Information System (INIS)

Atrio-Barandela, F.; Kashlinsky, A.

2014-01-01

The epoch of first star formation and the state of the intergalactic medium (IGM) at that time are not directly observable with current telescopes. The radiation from those early sources is now part of the cosmic infrared background (CIB) and, as these sources ionize the gas around them, the IGM plasma would produce faint temperature anisotropies in the cosmic microwave background (CMB) via the thermal Sunyaev-Zeldovich (TSZ) effect. While these TSZ anisotropies are too faint to be detected, we show that the cross-correlation of maps of source-subtracted CIB fluctuations from Euclid, with suitably constructed microwave maps at different frequencies, can probe the physical state of the gas during reionization and test/constrain models of the early CIB sources. We identify the frequency-combined, CMB-subtracted microwave maps from space- and ground-based instruments to show that they can be cross-correlated with the forthcoming all-sky Euclid CIB maps to detect the cross-power at scales ∼5'-60' with signal-to-noise ratios (S/Ns) of up to S/N ∼ 4-8 depending on the contribution to the Thomson optical depth during those pre-reionization epochs (Δτ ≅ 0.05) and the temperature of the IGM (up to ∼10 4 K). Such a measurement would offer a new window to explore the emergence and physical properties of these first light sources

Investigating cluster astrophysics and cosmology with cross-correlation of the thermal Sunyaev-Zel'dovich effect and weak lensing

Science.gov (United States)

Osato, Ken; Flender, Samuel; Nagai, Daisuke; Shirasaki, Masato; Yoshida, Naoki

2018-03-01

Recent detections of the cross-correlation of the thermal Sunyaev-Zel'dovich (tSZ) effect and weak gravitational lensing (WL) enable unique studies of cluster astrophysics and cosmology. In this work, we present constraints on the amplitude of the non-thermal pressure fraction in galaxy clusters, α0, and the amplitude of the matter power spectrum, σ8, using measurements of the tSZ power spectrum from Planck, and the tSZ-WL cross-correlation from Planck and the Red Cluster Sequence Lensing Survey. We fit the data to a semi-analytic model with the covariance matrix using N-body simulations. We find that the tSZ power spectrum alone prefers σ8 ˜ 0.85 and a large fraction of non-thermal pressure (α0 ˜ 0.2-0.3). The tSZ-WL cross-correlation on the other hand prefers a significantly lower σ8 ˜ 0.6 and low α0 ˜ 0.05. We show that this tension can be mitigated by allowing for a steep slope in the stellar mass-halo mass relation, which would cause a reduction of the gas in low-mass haloes. In such a model, the combined data prefer σ8 ˜ 0.7 and α0 ˜ 0.2, consistent with predictions from hydrodynamical simulations.

Quantification of fluorescence angiography in a porcine model

DEFF Research Database (Denmark)

Nerup, Nikolaj; Andersen, Helene Schou; Ambrus, Rikard

2017-01-01

PURPOSE: There is no consensus on how to quantify indocyanine green (ICG) fluorescence angiography. The aim of the present study was to establish and gather validity evidence for a method of quantifying fluorescence angiography, to assess organ perfusion. METHODS: Laparotomy was performed on seven...... pigs, with two regions of interest (ROIs) marked. ICG and neutron-activated microspheres were administered and the stomach was illuminated in the near-infrared range, parallel to continuous recording of fluorescence signal. Tissue samples from the ROIs were sent for quantification of microspheres...... to calculate the regional blood flow. A software system was developed to assess the fluorescent recordings quantitatively, and each quantitative parameter was compared with the regional blood flow. The parameter with the strongest correlation was then compared with results from an independently developed...

Correlating two-photon excited fluorescence imaging of breast cancer cellular redox state with seahorse flux analysis of normalized cellular oxygen consumption

Science.gov (United States)

Hou, Jue; Wright, Heather J.; Chan, Nicole; Tran, Richard; Razorenova, Olga V.; Potma, Eric O.; Tromberg, Bruce J.

2016-06-01

Two-photon excited fluorescence (TPEF) imaging of the cellular cofactors nicotinamide adenine dinucleotide and oxidized flavin adenine dinucleotide is widely used to measure cellular metabolism, both in normal and pathological cells and tissues. When dual-wavelength excitation is used, ratiometric TPEF imaging of the intrinsic cofactor fluorescence provides a metabolic index of cells-the "optical redox ratio" (ORR). With increased interest in understanding and controlling cellular metabolism in cancer, there is a need to evaluate the performance of ORR in malignant cells. We compare TPEF metabolic imaging with seahorse flux analysis of cellular oxygen consumption in two different breast cancer cell lines (MCF-7 and MDA-MB-231). We monitor metabolic index in living cells under both normal culture conditions and, for MCF-7, in response to cell respiration inhibitors and uncouplers. We observe a significant correlation between the TPEF-derived ORR and the flux analyzer measurements (R=0.7901, p<0.001). Our results confirm that the ORR is a valid dynamic index of cell metabolism under a range of oxygen consumption conditions relevant for cancer imaging.

Full genotyping of a highly polymorphic human gene trait by time-resolved fluorescence resonance energy transfer.

Directory of Open Access Journals (Sweden)

Edoardo Totè

Full Text Available The ability of detecting the subtle variations occurring, among different individuals, within specific DNA sequences encompassed in highly polymorphic genes discloses new applications in genomics and diagnostics. DQB1 is a gene of the HLA-II DQ locus of the Human Leukocyte Antigens (HLA system. The polymorphisms of the trait of the DQB1 gene including codons 52-57 modulate the susceptibility to a number of severe pathologies. Moreover, the donor-receiver tissue compatibility in bone marrow transplantations is routinely assessed through crossed genotyping of DQB and DQA. For the above reasons, the development of rapid, reliable and cost-effective typing technologies of DQB1 in general, and more specifically of the codons 52-57, is a relevant although challenging task. Quantitative assessment of the fluorescence resonance energy transfer (FRET efficiency between chromophores labelling the opposite ends of gene-specific oligonucleotide probes has proven to be a powerful tool to type DNA polymorphisms with single-nucleotide resolution. The FRET efficiency can be most conveniently quantified by applying a time-resolved fluorescence analysis methodology, i.e. time-correlated single-photon counting, which allows working on very diluted template specimens and in the presence of fluorescent contaminants. Here we present a full in-vitro characterization of the fluorescence responses of two probes when hybridized to oligonucleotide mixtures mimicking all the possible genotypes of the codons 52-57 trait of DQB1 (8 homozygous and 28 heterozygous. We show that each genotype can be effectively tagged by the combination of the fluorescence decay constants extrapolated from the data obtained with such probes.

Accessibility of nucleic acid-complexed biomolecules to hydroxyl radicals correlates with their conformation: a fluorescence polarization spectroscopy study

International Nuclear Information System (INIS)

Makrigiorgos, G.M.; Bump, E.; Huang, C.; Kassis, A.I.; Baranowska-Kortylewicz, J.

1994-01-01

A fluorescence methodology has been developed to examine the relationship between the conformational state of specific biomolecules in simple chromatin models and their accessibility to hydroxyl radicals ( . OH). Polylysine and histone H1 were labelled with SECCA, the succinimidyl ester of coumarin-3-carboxylic acid, which generates the fluorescent derivative 7-OH-SECCA following its interaction with radiation-induced . OH in aqueous solution. The fluorescence induced per unit γ-ray dose reflecting the accessibility of . OH to such SECCA-conjugated biomolecules was recorded. The biomolecules were also labelled with the fluorescent derivative 7-OH-SECCA in trace amounts to study their conformation under identical conditions via fluorescence polarization spectroscopy. (author)

Tomographic-spectral approach for dark matter detection in the cross-correlation between cosmic shear and diffuse γ-ray emission

International Nuclear Information System (INIS)

Camera, S.; Fornasa, M.; Fornengo, N.; Regis, M.

2015-01-01

We recently proposed to cross-correlate the diffuse extragalactic γ-ray background with the gravitational lensing signal of cosmic shear. This represents a novel and promising strategy to search for annihilating or decaying particle dark matter (DM) candidates. In the present work, we demonstrate the potential of a tomographic-spectral approach: measuring the cross-correlation in separate bins of redshift and energy significantly improves the sensitivity to a DM signal. Indeed, the technique proposed here takes advantage of the different scaling of the astrophysical and DM components with redshift and, simultaneously of their different energy spectra and different angular extensions. The sensitivity to a particle DM signal is extremely promising even when the DM-induced emission is quite faint. We first quantify the prospects of detecting DM by cross-correlating the Fermi Large Area Telescope (LAT) diffuse γ-ray background with the cosmic shear expected from the Dark Energy Survey. Under the hypothesis of a significant subhalo boost, such a measurement can deliver a 5σ detection of DM, if the DM particle is lighter than 300 GeV and has a thermal annihilation rate. We then forecast the capability of the European Space Agency Euclid satellite (whose launch is planned for 2020), in combination with an hypothetical future γ-ray detector with slightly improved specifications compared to current telescopes. We predict that the cross-correlation of their data will allow a measurement of the DM mass with an uncertainty of a factor of 1.5–2, even for moderate subhalo boosts, for DM masses up to few hundreds of GeV and thermal annihilation rates

Tomographic-spectral approach for dark matter detection in the cross-correlation between cosmic shear and diffuse γ-ray emission

Energy Technology Data Exchange (ETDEWEB)

Camera, S. [Jodrell Bank Centre for Astrophysics, The University of Manchester, Alan Turing Building, Oxford Road, Manchester M13 9PL (United Kingdom); Fornasa, M. [School of Physics and Astronomy, University of Nottingham, University Campus, Nottingham NG7 2RD (United Kingdom); Fornengo, N.; Regis, M., E-mail: stefano.camera@manchester.ac.uk, E-mail: fornasam@gmail.com, E-mail: fornengo@to.infn.it, E-mail: regis@to.infn.it [Dipartimento di Fisica, Università di Torino, Via P. Giuria 1, 10125 Torino (Italy)

2015-06-01

We recently proposed to cross-correlate the diffuse extragalactic γ-ray background with the gravitational lensing signal of cosmic shear. This represents a novel and promising strategy to search for annihilating or decaying particle dark matter (DM) candidates. In the present work, we demonstrate the potential of a tomographic-spectral approach: measuring the cross-correlation in separate bins of redshift and energy significantly improves the sensitivity to a DM signal. Indeed, the technique proposed here takes advantage of the different scaling of the astrophysical and DM components with redshift and, simultaneously of their different energy spectra and different angular extensions. The sensitivity to a particle DM signal is extremely promising even when the DM-induced emission is quite faint. We first quantify the prospects of detecting DM by cross-correlating the Fermi Large Area Telescope (LAT) diffuse γ-ray background with the cosmic shear expected from the Dark Energy Survey. Under the hypothesis of a significant subhalo boost, such a measurement can deliver a 5σ detection of DM, if the DM particle is lighter than 300 GeV and has a thermal annihilation rate. We then forecast the capability of the European Space Agency Euclid satellite (whose launch is planned for 2020), in combination with an hypothetical future γ-ray detector with slightly improved specifications compared to current telescopes. We predict that the cross-correlation of their data will allow a measurement of the DM mass with an uncertainty of a factor of 1.5–2, even for moderate subhalo boosts, for DM masses up to few hundreds of GeV and thermal annihilation rates.

Tomographic-spectral approach for dark matter detection in the cross-correlation between cosmic shear and diffuse γ-ray emission

Science.gov (United States)

Camera, S.; Fornasa, M.; Fornengo, N.; Regis, M.

2015-06-01

We recently proposed to cross-correlate the diffuse extragalactic γ-ray background with the gravitational lensing signal of cosmic shear. This represents a novel and promising strategy to search for annihilating or decaying particle dark matter (DM) candidates. In the present work, we demonstrate the potential of a tomographic-spectral approach: measuring the cross-correlation in separate bins of redshift and energy significantly improves the sensitivity to a DM signal. Indeed, the technique proposed here takes advantage of the different scaling of the astrophysical and DM components with redshift and, simultaneously of their different energy spectra and different angular extensions. The sensitivity to a particle DM signal is extremely promising even when the DM-induced emission is quite faint. We first quantify the prospects of detecting DM by cross-correlating the Fermi Large Area Telescope (LAT) diffuse γ-ray background with the cosmic shear expected from the Dark Energy Survey. Under the hypothesis of a significant subhalo boost, such a measurement can deliver a 5σ detection of DM, if the DM particle is lighter than 300 GeV and has a thermal annihilation rate. We then forecast the capability of the European Space Agency Euclid satellite (whose launch is planned for 2020), in combination with an hypothetical future γ-ray detector with slightly improved specifications compared to current telescopes. We predict that the cross-correlation of their data will allow a measurement of the DM mass with an uncertainty of a factor of 1.5-2, even for moderate subhalo boosts, for DM masses up to few hundreds of GeV and thermal annihilation rates.

Monitoring the Behavior of Emerging Contaminants in Wastewater-Impacted Rivers Based on the Use of Fluorescence Excitation Emission Matrixes (EEM).

Science.gov (United States)

Sgroi, Massimiliano; Roccaro, Paolo; Korshin, Gregory V; Vagliasindi, Federico G A

2017-04-18

This study investigated the applicability of fluorescence indexes based on the interpretation of excitation emission matrices (EEMs) by PARAFAC analysis and by selecting fluorescence intensities at a priori defined excitation/emission pairs as surrogates for monitoring the behavior of emerging organic compounds (EOCs) in two catchment basins impacted by wastewater discharges. Relevant EOC and EEM data were obtained for a 90 km stretch of the Simeto River, the main river in Sicily, and the smaller San Leonardo River, which was investigated for a 17 km stretch. The use of fluorescence indexes developed by these two different approaches resulted in similar observations. Changes of the fluorescence indexes that correspond to a group of humic-like fluorescing species were determined to be highly correlated with the concentrations of recalcitrant contaminants such as sucralose, sulfamethoxazole and carbamazepine, which are typical wastewater markers in river water. Changes of the fluorescence indexes related to tyrosine-like substances were well correlated with the concentrations of ibuprofen and caffeine, anthropogenic indicators of untreated wastewater discharges. Chemical oxygen demand and dissolved organic carbon concentrations were correlated with humic-like fluorescence indexes. The observed correlations were site-specific and characterized by different regression parameters for every collection event. Caffeine and carbamazepine showed correlations with florescence indexes in the San Leonardo River and in the alluvial plain stretch of the Simeto River, whereas sucralose, sulfamethoxazole and ibuprofen have always been well correlated in all the investigated river stretches. However, when data of different collection events from river stretches where correlations were observed were combined, good linear correlations were obtained for data sets generated via the normalization of the measured concentrations by the average value for the corresponding collection event

Remote sensing vegetation status by laser-induced fluorescence

International Nuclear Information System (INIS)

Günther, K.P.; Dahn, H.G.; Lüdeker, W.

1994-01-01

In November 1989 the EUREKA project LASFLEUR (EU 380) started as an European research effort to investigate the future application of far-field laser-induced plant fluorescence for synoptic, airborne environmental monitoring of vegetation. This report includes a brief introduction in a theoretically approach for the laser-induced fluorescence signals of leaves and their spectral and radiometric behaviour. In addition, a detailed description of the design and realization of the second generation of the far-field fluorescence lidar (DLidaR-2) is given with special regard to the optical and electronical setup, followed by a short explanation of the data processing. The main objectives of the far field measurements are to demonstrate the link between laser-induced fluorescence data and plant physiology and to show the reliability of remote single shot lidar measurements. The data sets include the typical daily cycles of the fluorescence for different global irradiation. As expected from biophysical models, the remotely sensed chlorophyll fluorescence is highly correlated with the carbon fixation rate, while the fluorescence ratio F685 / F730 is only dependent on the chlorophyll concentration. Drought stress measurement of evergreen oaks Quercus pubescens confirm the findings of healthy plants with regard to the fluorescence ratio F685 / F730 while the fluorescence signals of stressed plants show a different behavior than nonstressed plants. Additionally, the corresponding physiological data (porometer and PAM data) are presented. (author)

The properties of cross-correlation and spectra of the low-mass X-ray binary 4U 1608-52

International Nuclear Information System (INIS)

Lei, Ya-Juan; Yuan, Hai-Long; Dong, Yi-Qiao; Zhang, Hao-Tong; Zhang, Cheng-Min; Zhao, Yong-Heng; Zhang, Shu; Qu, Jin-Lu; Wang, Ya-Nan; Li, Zhi-Bing

2014-01-01

With RXTE data, we analyzed the cross-correlation function between the soft and hard X-rays of the transient atoll source 4U 1608-52. We found anti-correlations in three outbursts occurred in 1998, 2002, and 2010, and we found significant time lags of several hundreds of seconds in the latter two outbursts. Our results show no correlation between the soft and hard X-rays in the extreme island state and a dominated positive correlation in the lower banana state. Anti-correlations are presented at the upper banana state for the outburst of 2010 and at the island and the lower left banana states for the other two outbursts. So far for atoll sources, the cross-correlation has been studied statistically only for 4U 1735-44, where anti-correlations showed up in the upper banana state. Here our investigation on 4U 1608-52 provides a similar result in its 2010 outburst. In addition, we notice that the luminosities in the upper banana of the 1998 and 2002 outbursts are about 1.5 times that of the 2010 outburst whose luminosity in the upper banana is close to that of 4U 1735-44. The results suggest that the states in the color-color diagram of a source could be correlated with the luminosity of the source. A further spectral analysis during the 2010 outburst is also shown, which suggests that the disk can be a little truncated in the upper banana. The feature on the upper banana is similar to the previous results of the flaring branch in Z sources.

Coherent correlation enhancement of outer shell photoionization cross sections of alkali-like ions

International Nuclear Information System (INIS)

Amusia, M.Y.; Avdonina, B.; Pratt, R.H.

1995-01-01

An alkali-like ion interaction with inner electrons of an alkali-like ion leads to a significant increase in the photoionization cross section of the outer s electron. This occurs not only for ground-state ions with one s electron in the outer shell, but also when the outer s electron is in an excited state. The reason for this amplification, in addition to coherent enhancement in summing of the correlation amplitudes, is that the zero in the direct amplitude occurs below threshold. This leads to a constructive interference with the correlation amplitude above the photoionization threshold, in contrast to a destructive interference in the case of a neutral atom with the same electronic configuration, for which the zero occurs above threshold. Results of this research were published

A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

DEFF Research Database (Denmark)

Svendsen, Claus Bo; Boye, Mette; Struve, Carsten

2009-01-01

A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross-reactions with bact......A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Ricekttsia was found without problems of cross...

In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles

Directory of Open Access Journals (Sweden)

Zhang Y

2013-12-01

Full Text Available Yue Zhang,1 Bin Zhang,1 Fei Liu,1,2 Jianwen Luo,1,3 Jing Bai1 1Department of Biomedical Engineering, School of Medicine, 2Tsinghua-Peking Center for Life Sciences, 3Center for Biomedical Imaging Research, Tsinghua University, Beijing, People's Republic of China Abstract: Dual-modality imaging combines the complementary advantages of different modalities, and offers the prospect of improved preclinical research. The combination of fluorescence imaging and magnetic resonance imaging (MRI provides cross-validated information and direct comparison between these modalities. Here, we report on the application of a novel tumor-targeted, dual-labeled nanoparticle (NP, utilizing iron oxide as the MRI contrast agent and near infrared (NIR dye Cy5.5 as the fluorescent agent. Results of in vitro experiments verified the specificity of the NP to tumor cells. In vivo tumor targeting and uptake of the NPs in a mouse model were visualized by fluorescence and MR imaging collected at different time points. Quantitative analysis was carried out to evaluate the efficacy of MRI contrast enhancement. Furthermore, tomographic images were also acquired using both imaging modalities and cross-validated information of tumor location and size between these two modalities was revealed. The results demonstrate that the use of dual-labeled NPs can facilitate the dual-modal detection of tumors, information cross-validation, and direct comparison by combing fluorescence molecular tomography (FMT and MRI. Keywords: dual-modality, fluorescence molecular tomography (FMT, magnetic resonance imaging (MRI, nanoparticle

Azadioxatriangulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay

International Nuclear Information System (INIS)

Sørensen, Thomas Just; Thyrhaug, Erling; Szabelski, Mariusz; Gryczynski, Ignacy; Gryczynski, Zygmunt; Luchowski, Rafal; Laursen, Bo W

2013-01-01

Of the many optical bioassays available, sensing by fluorescence anisotropy has great advantages as it provides a sensitive, instrumentally simple, ratiometric method of detection. However, it is hampered by a severe limitation, as the emission lifetime of the label needs to be comparable to the correlation lifetime (tumbling time) of the biomolecule which is labelled. For proteins of moderate size this is on the order of 20–200 ns, which due to practical issues currently limits the choice of labels to the dansyl-type dyes and certain aromatic dyes. These have the significant drawback of UV/blue absorption and emission as well as an often significant solvent sensitivity. Here, we report the synthesis and characterization of a new fluorescent label for high molecular weight biomolecule assay based on the azadioxatriangulenium motif. The NHS ester of the long fluorescence lifetime, red-emitting fluorophore: azadioxatriangulenium (ADOTA-NHS) was conjugated to anti-rabbit Immunoglobulin G (antiIgG). The long fluorescence lifetime was exploited to determine the correlation time of the high molecular weight antibody and its complex with rabbit Immunoglobulin G (IgG) with steady-state fluorescence anisotropy and time-resolved methods: solution phase immuno-assay was performed following either steady-state or time-resolved fluorescence anisotropy. By performing a variable temperature experiment it was determined that the binding of the ligand resulted in an increase in correlation time of more than 75%, and an increase in the steady-state anisotropy of 18%. The results show that the triangulenium class of dyes can be used in anisotropy assay to detect binding events involving biomolecules of far larger size than what is possible with most other red-emitting organic dyes. (paper)

Azadioxatriangulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay

Science.gov (United States)

Just Sørensen, Thomas; Thyrhaug, Erling; Szabelski, Mariusz; Luchowski, Rafal; Gryczynski, Ignacy; Gryczynski, Zygmunt; Laursen, Bo W.

2013-06-01

Of the many optical bioassays available, sensing by fluorescence anisotropy has great advantages as it provides a sensitive, instrumentally simple, ratiometric method of detection. However, it is hampered by a severe limitation, as the emission lifetime of the label needs to be comparable to the correlation lifetime (tumbling time) of the biomolecule which is labelled. For proteins of moderate size this is on the order of 20-200 ns, which due to practical issues currently limits the choice of labels to the dansyl-type dyes and certain aromatic dyes. These have the significant drawback of UV/blue absorption and emission as well as an often significant solvent sensitivity. Here, we report the synthesis and characterization of a new fluorescent label for high molecular weight biomolecule assay based on the azadioxatriangulenium motif. The NHS ester of the long fluorescence lifetime, red-emitting fluorophore: azadioxatriangulenium (ADOTA-NHS) was conjugated to anti-rabbit Immunoglobulin G (antiIgG). The long fluorescence lifetime was exploited to determine the correlation time of the high molecular weight antibody and its complex with rabbit Immunoglobulin G (IgG) with steady-state fluorescence anisotropy and time-resolved methods: solution phase immuno-assay was performed following either steady-state or time-resolved fluorescence anisotropy. By performing a variable temperature experiment it was determined that the binding of the ligand resulted in an increase in correlation time of more than 75%, and an increase in the steady-state anisotropy of 18%. The results show that the triangulenium class of dyes can be used in anisotropy assay to detect binding events involving biomolecules of far larger size than what is possible with most other red-emitting organic dyes.

The cross-correlation of the CMB polarization and the 21-cm line fluctuations from cosmic reionization

NARCIS (Netherlands)

Tashiro, Hiroyuki; Aghanim, Nabila; Langer, Mathieu; Douspis, Marian; Zaroubi, Saleem

2008-01-01

The cosmic microwave background (CMB) polarization and the 21-cm line fluctuations are powerful probes of cosmological reionization. We study how the cross-correlation between the CMB polarization (E modes) and the 21-cm line fluctuations can be used to gain further understanding of the reionization

Spatiotemporal Ultrafast-Plasmon Control Based on Response Functions of Nanostructures Measured by Interferometric Cross-Correlation Microscopy

Directory of Open Access Journals (Sweden)

Kusaba Miyuki

2013-03-01

Full Text Available We demonstrate an electrical-field cross-correlation imaging technique to obtain a response function of localized plasmon generated by femtosecond laser pulses on gold nanostructures. Based on the measured response functions, we spatiotemporally control the plasmon by shaping the femtosecond excitation laser pulses.

Development of the loss coefficient correlation for cross flow between graphite fuel blocks in the core of prismatic very high temperature reactor-PMR200

International Nuclear Information System (INIS)

Lee, Jeong-Hun; Cho, Hyoung-Kyu; Park, Goon-Cherl

2016-01-01

Highlights: • Cross flow experimental data are produced with wedge-shaped and parallel gaps. • The results of a CFD analysis and experimental data are in good agreement. • Pressure loss coefficient for the cross gap between fuel blocks in PMR200 is found. • A new correlation of the cross flow loss coefficient for PMR200 is proposed. - Abstract: The core of the very high temperature reactor (VHTR) PMR200 (a prismatic modular reactor rated at 200 MW of thermal power) consists of hexagonal prismatic fuel blocks and reflector blocks made of graphite. If the core bypass flow ratio increases, the coolant channel flow is decreased and can then lower the heat removal efficiency, resulting in a locally increased fuel block temperature. The coolant channels in the fuel blocks are connected to bypass gaps by the cross gap, complicating flow distribution in the VHTR core. Therefore, reliable estimation of the bypass flow is highly important for the design and safety analysis of the VHTR core. Because of the complexity of the core geometry and gap configuration, it is challenging to predict the flow distribution in the VHTR core. To analyze this flow distribution accurately, it is necessary to determine the cross flow phenomena, and the loss coefficient across the cross gap has to be evaluated to determine the flow distribution in the VHTR core when a lumped parameter code or a flow network analysis code that uses the correlation of the loss coefficient is employed. The purpose of this paper is to develop a loss coefficient correlation applicable to the cross gap in the PMR200 core. The cross flow was evaluated experimentally using the difference between the measured inlet and outlet mass flow rates. Next, the applicability of a commercial computational fluid dynamics (CFD) code, CFX 15, was confirmed by comparing the experimental data and CFD analysis results. To understand the cross flow phenomena, the loss coefficient was evaluated; in the high Reynolds number region

Development of the loss coefficient correlation for cross flow between graphite fuel blocks in the core of prismatic very high temperature reactor-PMR200

Energy Technology Data Exchange (ETDEWEB)

Lee, Jeong-Hun, E-mail: huny12@snu.ac.kr; Cho, Hyoung-Kyu, E-mail: chohk@snu.ac.kr; Park, Goon-Cherl, E-mail: parkgc@snu.ac.kr

2016-10-15

Highlights: • Cross flow experimental data are produced with wedge-shaped and parallel gaps. • The results of a CFD analysis and experimental data are in good agreement. • Pressure loss coefficient for the cross gap between fuel blocks in PMR200 is found. • A new correlation of the cross flow loss coefficient for PMR200 is proposed. - Abstract: The core of the very high temperature reactor (VHTR) PMR200 (a prismatic modular reactor rated at 200 MW of thermal power) consists of hexagonal prismatic fuel blocks and reflector blocks made of graphite. If the core bypass flow ratio increases, the coolant channel flow is decreased and can then lower the heat removal efficiency, resulting in a locally increased fuel block temperature. The coolant channels in the fuel blocks are connected to bypass gaps by the cross gap, complicating flow distribution in the VHTR core. Therefore, reliable estimation of the bypass flow is highly important for the design and safety analysis of the VHTR core. Because of the complexity of the core geometry and gap configuration, it is challenging to predict the flow distribution in the VHTR core. To analyze this flow distribution accurately, it is necessary to determine the cross flow phenomena, and the loss coefficient across the cross gap has to be evaluated to determine the flow distribution in the VHTR core when a lumped parameter code or a flow network analysis code that uses the correlation of the loss coefficient is employed. The purpose of this paper is to develop a loss coefficient correlation applicable to the cross gap in the PMR200 core. The cross flow was evaluated experimentally using the difference between the measured inlet and outlet mass flow rates. Next, the applicability of a commercial computational fluid dynamics (CFD) code, CFX 15, was confirmed by comparing the experimental data and CFD analysis results. To understand the cross flow phenomena, the loss coefficient was evaluated; in the high Reynolds number region

Community detection for fluorescent lifetime microscopy image segmentation

Science.gov (United States)

Hu, Dandan; Sarder, Pinaki; Ronhovde, Peter; Achilefu, Samuel; Nussinov, Zohar

2014-03-01

Multiresolution community detection (CD) method has been suggested in a recent work as an efficient method for performing unsupervised segmentation of fluorescence lifetime (FLT) images of live cell images containing fluorescent molecular probes.1 In the current paper, we further explore this method in FLT images of ex vivo tissue slices. The image processing problem is framed as identifying clusters with respective average FLTs against a background or "solvent" in FLT imaging microscopy (FLIM) images derived using NIR fluorescent dyes. We have identified significant multiresolution structures using replica correlations in these images, where such correlations are manifested by information theoretic overlaps of the independent solutions ("replicas") attained using the multiresolution CD method from different starting points. In this paper, our method is found to be more efficient than a current state-of-the-art image segmentation method based on mixture of Gaussian distributions. It offers more than 1:25 times diversity based on Shannon index than the latter method, in selecting clusters with distinct average FLTs in NIR FLIM images.

Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy.

Directory of Open Access Journals (Sweden)

Ronan Broderick

Full Text Available Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our data show that eGFP-Cdc45 mobility is faster in G1/S transition compared to S phase suggesting that Cdc45 is part of larger protein complex formed in S phase. Furthermore, the size of complexes containing Cdc45 was estimated in asynchronous, G1/S and S phase-synchronized cells using gel filtration chromatography; these findings complemented the in vivo FCS data. Analysis of the mobility of eGFP-Cdc45 and the size of complexes containing Cdc45 and eGFP-Cdc45 after UVC-mediated DNA damage revealed no significant changes in diffusion rates and complex sizes using FCS and gel filtration chromatography analyses. This suggests that after UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage.

Impulse response measurements with an off-line cross correlator

International Nuclear Information System (INIS)

Corran, E.R.; Cummins, J.D.

1963-11-01

The impulse responses of simulated systems have been computed by off-line cross-correlation of the system input and output signals. The input test signal consisted of a discrete interval binary code whose autocorrelation was a triangular pulse at zero lag. The main object of the experiments was to study the inaccuracies introduced in ideal, noise free systems by determining the impulse response digitally from sampled versions of the system input and output signals. A second object was to determine the error introduced by adding controlled amounts of uncorrelated noise at the system outputs. The experimental results showed that for signal to noise ratios greater than 10:1 in the mean square sense, the impulse responses may be determined with reasonable accuracy using only one cycle of the binary code. The method lends itself to on-line computation of system impulse responses. The latter could be used to monitor the stability of the system or to determine control parameters in an adaptive control system. (author)