WorldWideScience

Sample records for flow separation location

  1. Flow Separation

    Science.gov (United States)

    1975-11-01

    Born, Constantin Caratheodory, Richard Couiant, Kurt Friedrichs, Werner Heisenberg, Gustav Herglotz, Erich von Hoist, Pascual Jordan, Walther Nernst...existence of these two flow regimes in boundary layers was discovered by PRANDTL when EIFFEL [8] published in 1912 his measurements on the drag of...simultaneously by G. EIFFEL in Paris and became so successful that other wind tunnels were modelled after it in many countries. Fig. 18 gives an impression of

  2. Separation of flow

    CERN Document Server

    Chang, Paul K

    2014-01-01

    Interdisciplinary and Advanced Topics in Science and Engineering, Volume 3: Separation of Flow presents the problem of the separation of fluid flow. This book provides information covering the fields of basic physical processes, analyses, and experiments concerning flow separation.Organized into 12 chapters, this volume begins with an overview of the flow separation on the body surface as discusses in various classical examples. This text then examines the analytical and experimental results of the laminar boundary layer of steady, two-dimensional flows in the subsonic speed range. Other chapt

  3. Thermographic Detection of separated Flow

    Science.gov (United States)

    Dollinger, C.; Balaresque, N.; Schaffarczyk, A. P.; Fischer, A.

    2016-09-01

    Thermographic wind tunnel measurements, both on a cylinder as well as on a 2D airfoil, were performed at various Reynolds numbers in order to evaluate the possibility of detecting and visualizing separated flow areas. A new approach by acquiring a series of thermographic images and applying a spatial-temporal statistical analysis allows improving both the resolution and the information content of the thermographic images. Separated flow regions become visible and laminar/turbulent transitions can be detected more accurately. The knowledge about possibly present stall cells can be used to confirm two-dimensional flow conditions and support the development of more effective and silent rotorblades.

  4. Microfluidic ultrasonic particle separators with engineered node locations and geometries

    Energy Technology Data Exchange (ETDEWEB)

    Rose, Klint A.; Fisher, Karl A.; Wajda, Douglas A.; Mariella, Jr., Raymond P.; Bailey, Christopher; Dehlinger, Dietrich; Shusteff, Maxim; Jung, Byoungsok; Ness, Kevin D.

    2016-04-26

    An ultrasonic microfluidic system includes a separation channel for conveying a sample fluid containing small particles and large particles, flowing substantially parallel, adjacent to a recovery fluid, with which it is in contact. An acoustic transducer produces an ultrasound standing wave, that generates a pressure field having at least one node of minimum pressure amplitude. An acoustic extension structure is located proximate to said separation channel for positioning said acoustic node off center in said acoustic area and concentrating the large particles in said recovery fluid stream.

  5. Gradient Flow Convolutive Blind Source Separation

    DEFF Research Database (Denmark)

    Pedersen, Michael Syskind; Nielsen, Chinton Møller

    2004-01-01

    Experiments have shown that the performance of instantaneous gradient flow beamforming by Cauwenberghs et al. is reduced significantly in reverberant conditions. By expanding the gradient flow principle to convolutive mixtures, separation in a reverberant environment is possible. By use of a circ......Experiments have shown that the performance of instantaneous gradient flow beamforming by Cauwenberghs et al. is reduced significantly in reverberant conditions. By expanding the gradient flow principle to convolutive mixtures, separation in a reverberant environment is possible. By use...

  6. Composite separators and redox flow batteries based on porous separators

    Science.gov (United States)

    Li, Bin; Wei, Xiaoliang; Luo, Qingtao; Nie, Zimin; Wang, Wei; Sprenkle, Vincent L.

    2016-01-12

    Composite separators having a porous structure and including acid-stable, hydrophilic, inorganic particles enmeshed in a substantially fully fluorinated polyolefin matrix can be utilized in a number of applications. The inorganic particles can provide hydrophilic characteristics. The pores of the separator result in good selectivity and electrical conductivity. The fluorinated polymeric backbone can result in high chemical stability. Accordingly, one application of the composite separators is in redox flow batteries as low cost membranes. In such applications, the composite separator can also enable additional property-enhancing features compared to ion-exchange membranes. For example, simple capacity control can be achieved through hydraulic pressure by balancing the volumes of electrolyte on each side of the separator. While a porous separator can also allow for volume and pressure regulation, in RFBs that utilize corrosive and/or oxidizing compounds, the composite separators described herein are preferable for their robustness in the presence of such compounds.

  7. Recent Development in Flow Separation.

    Science.gov (United States)

    1980-05-01

    indicates the differentiation with respect to x, and thr prime over f with respect to ry substituting eq.(3) into eq.(2), eliminating the derivative...negligible. Erdos and Zakkay’s (1971) flow model, indicates that although vortices are created in the base re- gion, viscous stresses and diffusion may not be...and it is hoped that a few iterations at most will result in "convergent" and useful solution. Erdos and Zakkay (1971), analyzed the steady laminar

  8. Efficient Indoor Proximity and Separation Detection for Location Fingerprinting

    DEFF Research Database (Denmark)

    Kjærgaard, Mikkel Baun; Treu, Georg; Ruppel, Peter

    2008-01-01

    Detecting proximity and separation among mobile targets is a basic mechanism for many location-based services (LBSs) and requires continuous positioning and tracking. However, realizing both mechanisms for indoor usage is still a major challenge. Positioning methods like GPS cannot be applied there......, and for distance calculations the particular building topology has to be taken into account. To address these challenges, this paper presents a novel approach for indoor proximity and separation detection, which uses location fingerprinting for indoor positioning of targets and walking distances for modeling...

  9. Separation enhancement in pinched flow fractionation

    DEFF Research Database (Denmark)

    Vig, Asger Laurberg; Kristensen, Anders

    2008-01-01

    A method for enhancing the separation in the microfluidic size separation technique called pinched flow fractionation (PFF) is demonstrated experimentally and analyzed by numerical calculations. The enhancement is caused by a geometrical modification of the original PFF design. Seven different...... polystyrene bead sizes ranging from 0.25 to 2.5 mu m in radius were separated in a PFF and in an enhanced PFF device. The separation in the two types of devices were compared and an amplification in the separation of up to 70% was achieved. Numerical calculations, which include an edge effect, are used...

  10. Flow pattern in reverse-flow centrifugal separators

    NARCIS (Netherlands)

    Peng, W; Hoffmann, AC; Boot, PJAJ; Udding, A; Dries, HWA; Ekker, A; Kater, J

    2002-01-01

    Experimental flow patterns, determined by Laser Doppler Anemometry (LDA) for two types of reverse-flow centrifugal separators, are presented. The flow patterns in (a) a conventional cylinder-on-cone cyclone with tangential inlet and (b) a swirl tube with vane-generated swirl and a cylindrical body a

  11. Efficient Indoor Proximity and Separation Detection for Location Fingerprinting

    DEFF Research Database (Denmark)

    Kjærgaard, Mikkel Baun; Treu, Georg; Ruppel, Peter

    2008-01-01

    , and for distance calculations the particular building topology has to be taken into account. To address these challenges, this paper presents a novel approach for indoor proximity and separation detection, which uses location fingerprinting for indoor positioning of targets and walking distances for modeling...

  12. Separation mechanisms and fluid flow in oil/water separation

    Energy Technology Data Exchange (ETDEWEB)

    Celius, H.K.; Knudsen, B. [IKU Petroleumsforskning A/S, Trondheim (Norway); Hafskjold, B.; Hansen, E.W. [Selskapet for Industriell og Teknisk Forskning, Trondheim (Norway)

    1996-12-31

    This paper describes work aimed at physical and numerical modeling of separation rates of oil/water systems in order to establish better tools for design and operation of offshore operators. This work aims to integrate the chemical and physical phenomena behind coalescence and settling with those of fluid flow in the system, in order to develop tools for design and operational analysis of separation equipment. The work includes the development of a high pressure, bench-scale test rig to perform separation tests on live oil and water samples, and a rationale in the form of a computer code that can be used to interpret the test results and transform them to a form siutable for operational purposes. This involves a formulation of a mathematical description of the chemical and physical mechanisms behind the emulsification and separation process, and to establish a link to the hydrdynamic properties of the separator vessel. The Emucol computer program is used in the analysis. 12 refs., 5 figs.

  13. Flow separation on wind turbines blades

    Science.gov (United States)

    Corten, G. P.

    2001-01-01

    separation line, which causes the terms with the chord-wise speed or accelerations to disappear. The conclusion is that the chord-wise pressure gradient balances the Coriolis force. By doing so we obtain a simple set of equations that can be solved analytically. Subsequently, our model predicts that the convective term with the radial velocity (vrvr/r) is dominant in the equation for the r-direction, precisely the term that was neglected in Snel's analysis. 3. Multiple Power Levels Several large commercial wind turbines demonstrate drops in maximum power levels up to 45%, under apparently equal conditions. Earlier studies attempting to explain this effect by technical malfunctioning, aerodynamic instabilities and blade contamination effects estimated with computational fluid dynamics, have not yet yielded a plausible result. We formulated many hypotheses, three of which were useful. By taking stall flag measurements and making two other crucial experiments, we could confirm one of those three hypotheses: the insect hypothesis. Insects only fly in low wind, impacting upon the blades at specific locations. In these conditions, the insectual remains are located at positions where roughness has little influence on the profile performance, so that the power is not affected. In high winds however, the flow around the blades has changed. As a result, the positions at which the insects have impacted at low winds are very sensitive to contamination. So the contamination level changes at low wind when insects fly and this level determines the power in high winds when insects do not fly. As a consequence we get discrete power levels in high winds. The other two hypotheses, which did not cause the multiple power levels for the case we studied, gave rise to two new insights. First, we expect the power to depend on the wind direction at sites where the shape of the terrain concentrates the wind. In this case the power level of all turbine types, including pitch regulated ones, will be

  14. Flow in a rotating membrane plasma separator.

    Science.gov (United States)

    Lueptow, R M; Hajiloo, A

    1995-01-01

    Rotating filter separators are very effective in the separation of plasma from whole blood, but details of the flow field in the device have not been investigated. The flow in a commercial device has been modeled computationally using the finite element code FIDAP. Taylor vortices appear in the upstream end of the annulus but disappear in the downstream end because of increasing blood viscosity as plasma is removed. Fluid transport at the upstream end of the annulus results from both translation of Taylor vortices and fluid winding around the vortices. If the inertial effects of the axial flow are reduced, less fluid winds around the vortices and more fluid is transported by the translation of the vortices. The pressure at the membrane is nonuniform in the region where vortices appear, although the relative magnitude of the fluctuations is small.

  15. Gas-Liquid Flows and Phase Separation

    Science.gov (United States)

    McQuillen, John

    2004-01-01

    Common issues for space system designers include:Ability to Verify Performance in Normal Gravity prior to Deployment; System Stability; Phase Accumulation & Shedding; Phase Separation; Flow Distribution through Tees & Manifolds Boiling Crisis; Heat Transfer Coefficient; and Pressure Drop.The report concludes:Guidance similar to "A design that operates in a single phase is less complex than a design that has two-phase flow" is not always true considering the amount of effort spent on pressurizing, subcooling and phase separators to ensure single phase operation. While there is still much to learn about two-phase flow in reduced gravity, we have a good start. Focus now needs to be directed more towards system level problems .

  16. Some numerical analyses of flows with separation

    Science.gov (United States)

    Kuwahara, K.

    Computational study of high Reynolds number flow with large separation is the most important problem now in computational aerodynamics. Some examples of recent results of studies of such flows are presented. The methods used are finite-difference methods and vortex methods. The first example is that of an incompressible flow past two cylinders computed by the finite-difference method based on a stream function-vorticity formulation of the Navier-Stokes equations. It is pointed out that special care must be taken to determine the boundary values of the stream function on the bodies when using this method. The second example is that of an incompressible flow past a circular cylinder at a critical Reynolds number computed by the MAC method using an improved upwind difference. The sharp drag reduction is first captured numerically by this computation. Third is an example of the simplest version of the new vortex methods without conformal mapping, which enables us to compute incompressible flows with large separation very easily and economically. The last example is of a transonic flow past an oscillating airfoil computed by the Beam-Warming scheme.

  17. Flow separation characteristics of unstable dispersions

    Science.gov (United States)

    Voulgaropoulos, Victor; Zhai, Lusheng; Angeli, Panagiota

    2016-11-01

    Drops of a low viscosity oil are introduced through a multi-capillary inlet during the flow of water in a horizontal pipe. The flow rates of the continuous water phase are kept in the turbulent region while the droplets are injected at similar flow rates (with oil fractions ranging from 0.15 to 0.60). The acrylic pipe (ID of 37mm) is approximately 7m long. Measurements are conducted at three different axial locations to illustrate how the flow structures are formed and develop along the pipe. Initial observations are made on the flow patterns through high-speed imaging. Stratification is observed for the flow rates studied, indicating that the turbulent dispersive forces are lower than the gravity ones. These results are complemented with a tomography system acquiring measurements at the same locations and giving the cross-sectional hold-up. The coalescence dynamics are strong in the dense-packed drop layer and thus measurements with a dual-conductance probe are conducted to capture any drop size changes. It is found that the drop size variations depend on the spatial configuration of the drops, the initial drop size along with the continuous and dispersed phase velocities. Project funded under Chevron Energy Technology.

  18. Separated flow past smooth slender bodies

    Science.gov (United States)

    Williams, Ann Louise

    1991-02-01

    This dissertation describes an investigation of the separated flow past slender bodies at high angles of attack. Flows of this type occur on aircraft and missile forebodies and can develop large forces which are important when considering stability and control of the vehicle. The objective of this work is to extend the vortex sheet model, which has previously been implemented for slender wings and circular and elliptic cones, to cones of more general cross-section and to non-conical bodies. The cross-sections of the bodies studied here are basically square or triangular, but with rounded corners. The model is inviscid, so the separation positions must be prescribed. Two distinct families of solutions have been identified. For laterally symmetric configurations with symmetric separation positions and no yaw, the first family solutions are symmetric, whereas the second family solutions are asymmetric. For elliptic cones, it is known that cross-section thickness affects the degree of asymmetry of the flow and this represents a mechanism for the control of side forces. Square or triangular cross-sections with rounded corners are of interest to aerodynamicists and have been investigated to assess the effect on asymmetry of making a circular cross-section 'square' or 'triangular'. For 'square' and 'triangular' cones placed either side, or corner on to the flow, results are obtained which enable the effect of cross-section shape on the degree of asymmetry to be assessed. A non-conical vortex sheet model has been developed for the first time for separation from a smooth body. Previously a non-conical line-vortex model was implemented, however lack of representation of vorticity near the separation line limits the applicability of the results. The solution procedure for the non-conical problem consists of a downstream-marching scheme starting from a known solution at the nose. Starting solutions are available if the flow at the nose is assumed conical. With symmetry

  19. Tidal flow separation at protruding beach nourishments

    Science.gov (United States)

    Radermacher, Max; de Schipper, Matthieu A.; Swinkels, Cilia; MacMahan, Jamie H.; Reniers, Ad J. H. M.

    2017-01-01

    In recent years, the application of large-scale beach nourishments has been discussed, with the Sand Motor in the Netherlands as the first real-world example. Such protruding beach nourishments have an impact on tidal currents, potentially leading to tidal flow separation and the generation of tidal eddies of length scales larger than the nourishment itself. The present study examines the characteristics of the tidal flow field around protruding beach nourishments under varying nourishment geometry and tidal conditions, based on extensive field observations and numerical flow simulations. Observations of the flow field around the Sand Motor, obtained with a ship-mounted current profiler and a set of fixed current profilers, show that a tidal eddy develops along the northern edge of the mega-nourishment every flood period. The eddy is generated around peak tidal flow and gradually gains size and strength, growing much larger than the cross-shore dimension of the coastline perturbation. Based on a 3 week measurement period, it is shown that the intensity of the eddy modulates with the spring-neap tidal cycle. Depth-averaged tidal currents around coastline perturbations are simulated and compared to the field observations. The occurrence and behavior of tidal eddies is derived for a large set of simulations with varying nourishment size and shape. Results show that several different types of behavior exist, characterized by different combinations of the nourishment aspect ratio, the size of the nourishment relative to the tidal excursion length, and the influence of bed friction.

  20. Tidal flow separation at protruding beach nourishments

    OpenAIRE

    Rademacher, Max; de Schipper, Matthieu A.; Swinkels, Cilia; MacMahan, Jamie H.; Reniers, Ad J.H.M.

    2017-01-01

    The article of record as published may be found at http://dx.doi.org/10.1002/2016JC011942 In recent years, the application of large-scale beach nourishments has been discussed, with the Sand Motor in the Netherlands as the first real-world example. Such protruding beach nourishments have an impact on tidal currents, potentially leading to tidal flow separation and the generation of tidal eddies of length scales larger than the nourishment itself. The present study examines the cha...

  1. Analysis and representation of complex structures in separated flows

    Science.gov (United States)

    Helman, James; Hesselink, Lambertus

    1991-01-01

    We discuss our recent work on extraction and visualization of topological information in separated fluid flow data sets. As with scene analysis, an abstract representation of a large data set can greatly facilitate the understanding of complex, high-level structures. When studying flow topology, such a representation can be produced by locating and characterizing critical points in the velocity field and generating the associated stream surfaces. In 3D flows, the surface topology serves as the starting point. The 2D tangential velocity field near the surface of the body is examined for critical points. The tangential velocity field is integrated out along the principal directions of certain classes of critical points to produce curves depicting the topology of the flow near the body. The points and curves are linked to form a skeleton representing the 2D vector field topology. This skeleton provides a basis for analyzing the 3D structures associated with the flow separation. The points along the separation curves in the skeleton are used to start tangent curve integrations. Integration origins are successively refined to produce stream surfaces. The map of the global topology is completed by generating those stream surfaces associated with 3D critical points.

  2. Numerical Investigation on the Separated Flow of Axial Flow Stator in Diagonal Flow Fan

    Science.gov (United States)

    Kinoue, Yoichi; Shiomi, Norimasa; Setoguchi, Toshiaki; Kaneko, Kenji; Jin, Yingzi

    2010-06-01

    Experimental and numerical investigations were conducted for the internal flow of the stator of the diagonal flow fan. Corner separation near the hub surface and the suction surface of the stator blade are focused on. At low flow rate of 80% of the design flow rate, the corner separation between the suction surface and the hub surface can be found in both experimental and numerical results. Separation vortices are found in the computed oil flow on both suction and hub surfaces at 80% of the design flow rate in the three-dimensional numerical simulation.

  3. Estimation of roughness lengths and flow separation over compound bedforms in a natural-tidal inlet

    DEFF Research Database (Denmark)

    Lefebvre, Alice; Ernstsen, Verner Brandbyge; Winter, Christian

    2013-01-01

    as hydraulic roughness elements in dependence with flow direction. The presence of a flow separation zone on the bedform lee was estimated through analysis of the measured velocity directions and the calculation of the flow separation line. The Law of the Wall was used to calculate roughness lengths and shear...... velocities from log–linear segments sought on transect-averaged and single-location velocity profiles. During the ebb tide a permanent flow separation zone was established over the steep (10–20°) lee sides of the ebb-oriented primary bedforms, which generated a consequent drag on the flow. During the flood......, no flow separation was induced by the gentle (2°) lee side of the primary bedforms except over the steepest (10°) part of the lee side where a small separation zone was sometimes observed. As a result, hydraulic roughness was only due to the superimposed bedforms. The parameterized flow separation line...

  4. Experimental Research on Flow Separation Control using Synthetic Jet Actuators

    NARCIS (Netherlands)

    Koopmans, E.; Hoeijmakers, H.W.M.

    2014-01-01

    Airplane wings can suffer from flow separation, which greatly decreases their aerodynamic per-formance. The flow separates due to the bound-ary layer possessing insufficient momentum to engage the adverse pressure gradient along the airfoil surface. Flow separation control actively influences the fl

  5. Experimental research on flow separation control using synthetic jet actuators

    NARCIS (Netherlands)

    Koopmans, E.; Hoeijmakers, H.W.M.

    2014-01-01

    Airplane wings can suffer from flow separation, which greatly decreases their aerodynamic per-formance. The flow separates due to the bound-ary layer possessing insufficient momentum to engage the adverse pressure gradient along the airfoil surface. Flow separation control actively influences the fl

  6. A parameterization of flow separation over subaqueous dunes

    NARCIS (Netherlands)

    Paarlberg, Andries J.; Dohmen-Janssen, C. Marjolein; Hulscher, Suzanne J.M.H.; Termes, Paul

    2007-01-01

    Flow separation plays a key role in the development of dunes, and modeling the complicated flow behavior inside the flow separation zone requires much computational effort. To make a first step toward modeling dune development at reasonable temporal and spatial scales, a parameterization of the shap

  7. A parameterization of flow separation over sub-aqueous dunes

    NARCIS (Netherlands)

    Paarlberg, Andries; Dohmen-Janssen, Catarine M.; Hulscher, Suzanne J.M.H.; Termes, Paul

    2007-01-01

    Flow separation plays a key role in the development of dunes, and modeling the complicated flow behavior inside the flow separation zone requires much computational effort. To make a first step toward modeling dune development at reasonable temporal and spatial scales, a parameterization of the

  8. A mechanical model for phase-separation in debris flow

    CERN Document Server

    Pudasaini, Shiva P

    2016-01-01

    Understanding the physics of phase-separation between solid and fluid phases as a mixture mass moves down slope is a long-standing challenge. Here, we propose an extension of the two phase mass flow model (Pudasaini, 2012) by including a new mechanism, called separation-flux, that leads to strong phase-separation in avalanche and debris flows while balancing the enhanced solid flux with the reduced fluid flux. The separation flux mechanism is capable of describing the dynamically evolving phase-separation and levee formation in a multi-phase, geometrically three-dimensional debris flow. These are often observed phenomena in natural debris flows and industrial processes that involve the transportation of particulate solid-fluid mixture material. The novel separation-flux model includes several dominant physical and mechanical aspects such as pressure gradients, volume fractions of solid and fluid phases and their gradients, shear-rates, flow depth, material friction, viscosity, material densities, topographic ...

  9. Investigation of several passive and active methods for turbulent flow separation control

    Science.gov (United States)

    Lin, J. C.; Howard, F. G.; Bushnell, D. M.; Selby, G. V.

    1990-01-01

    Relative performance of several passive and active methods for controlling two-dimensional turbulent separated flow associated with a curved backward-facing ramp were investigated at low speeds. Surface static pressure measurement and oil flow visualization results indicate that submerged vortex generators, vortex generator jets, elongated arches at +-alpha, and large-eddy breakup devices at +-alpha placed near the baseline separation location reduce flow separation and increase pressure recovery. Spanwise cylinders reduce flow separation but decrease pressure recovery downstream. Arches with alpha = 0 deg, Helmholtz resonators, and Viets' fluidic flappers examined so far have no significant effect in reducing separation. Wall cooling computation indicates that separation delay on a partially cooled ramp is nearly the same as on a fully-cooled ramp while minimizing the frictional drag increase associated with the wall cooling process.

  10. Flow separation in rocket nozzles under high altitude condition

    Science.gov (United States)

    Stark, R.; Génin, C.

    2017-01-01

    The knowledge of flow separation in rocket nozzles is crucial for rocket engine design and optimum performance. Typically, flow separation is studied under sea-level conditions. However, this disregards the change of the ambient density during ascent of a launcher. The ambient flow properties are an important factor concerning the design of altitude-adaptive rocket nozzles like the dual bell nozzle. For this reason an experimental study was carried out to study the influence of the ambient density on flow separation within conventional nozzles.

  11. Separation system with a sheath-flow supported electrochemical detector

    Science.gov (United States)

    Mathies, Richard A.; Emrich, Charles A.; Singhal, Pankaj; Ertl, Peter

    2008-10-21

    An electrochemical detector including side channels associated with a separation channel of a sample component separation apparatus is provided. The side channels of the detector, in one configuration, provide a sheath-flow for an analyte exiting the separation channel which directs the analyte to the electrically developed electrochemical detector.

  12. NUMERICAL SIMULATION OF SEPARATED FLOW NEAR GROYNE

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A numerical model was developed to simulate flow around non-submeged groyne in two dimensions, which was based on N-S equations with Smagorinsky's subgrid-scale turbulence model. Flow phenomenon and results measured practically agree with the calculation results very well, and this model could be used to simulate the characteristics of the eddies of upper and down reaches around spur-dikes successfully.

  13. Topology of Flow Separation on Three-Dimensional Bodies

    Science.gov (United States)

    Chapman, Gary T.; Yates, Leslie A.

    1991-01-01

    In recent years there has been extensive research on three-dimensional flow separation. There are two different approaches: the phenomenological approach and a mathematical approach using topology. These two approaches are reviewed briefly and the shortcomings of some of the past works are discussed. A comprehensive approach applicable to incompressible and compressible steady-state flows as well as incompressible unsteady flow is then presented. The approach is similar to earlier topological approaches to separation but is more complete and in some cases adds more emphasis to certain points than in the past. To assist in the classification of various types of flow, nomenclature is introduced to describe the skin-friction portraits on the surface. This method of classification is then demonstrated on several categories of flow to illustrate particular points as well as the diversity of flow separation. The categories include attached, two-dimensional separation and three different types of simple, three-dimensional primary separation, secondary separation, and compound separation. Hypothetical experiments are utilized to illustrate the topological terminology and its role in characterizing these flows. These hypothetical experiments use colored oil injected onto the surface at singular points in the skin-friction portrait. Actual flow-visualization information, if available, is used to corroborate the hypothetical examples.

  14. 5th International Conference on Jets, Wakes and Separated Flows

    CERN Document Server

    2016-01-01

    This volume collects various contributions from the 5th International Conference on Jets, Wakes and Separated Flows (ICJWSF2015) that took place in Stockholm during June 2015. Researchers from all around the world presented their latest results concerning fundamental and applied aspects of fluid dynamics. With its general character, the conference embraced many aspects of fluid dynamics, such as shear flows, multiphase flows and vortex flows, for instance. The structure of the present book reflects the variety of topics treated within the conference i.e. Jets, Wakes, Separated flows, Vehicle aerodynamics, Wall-bounded and confined flows, Noise, Turbomachinery flows, Multiphase and reacting flows, Vortex dynamics, Energy-related flows and a section dedicated to Numerical analyses.

  15. Flow Separation and Turbulence in Jet Pumps for Thermoacoustic Applications

    NARCIS (Netherlands)

    Oosterhuis, Joris P.; Verbeek, Anton A.; Bühler, Simon; Wilcox, Douglas; Meer, van der Theo H.

    2016-01-01

    The effect of flow separation and turbulence on the performance of a jet pump in oscillatory flows is investigated. A jet pump is a static device whose shape induces asymmetric hydrodynamic end effects when placed in an oscillatory flow. This will result in a time-averaged pressure drop which can be

  16. Differential Reynolds stress modeling for separating flows in industrial aerodynamics

    CERN Document Server

    2015-01-01

    This book presents recent progress in the application of RANS turbulence models based on the Reynolds stress transport equations. A variety of models has been implemented by different groups into different flow solvers and applied to external as well as to turbomachinery flows. Comparisons between the models allow an assessment of their performance in different flow conditions. The results demonstrate the general applicability of differential Reynolds stress models to separating flows in industrial aerodynamics.

  17. Turbulence Modeling of Flows with Extensive Crossflow Separation

    Directory of Open Access Journals (Sweden)

    Argyris G. Panaras

    2015-07-01

    Full Text Available The reasons for the difficulty in simulating accurately strong 3-D shock wave/turbulent boundary layer interactions (SBLIs and high-alpha flows with classical turbulence models are investigated. These flows are characterized by the appearance of strong crossflow separation. In view of recent additional evidence, a previously published flow analysis, which attributes the poor performance of classical turbulence models to the observed laminarization of the separation domain, is reexamined. According to this analysis, the longitudinal vortices into which the separated boundary layer rolls up in this type of separated flow, transfer external inviscid air into the part of the separation adjacent to the wall, decreasing its turbulence. It is demonstrated that linear models based on the Boussinesq equation provide solutions of moderate accuracy, while non-linear ones and others that consider the particular structure of the flow are more efficient. Published and new Reynolds Averaged Navier–Stokes (RANS simulations are reviewed, as well as results from a recent Large Eddy Simulation (LES study, which indicate that in calculations characterized by sufficient accuracy the turbulent kinetic energy of the reverse flow inside the separation vortices is very low, i.e., the flow is almost laminar there.

  18. International Conference on Instability and Control of Massively Separated Flows

    CERN Document Server

    Soria, Julio

    2015-01-01

    This book contains the outcome of the international meeting on instability, control and noise generated by massive flow separation that was organized at the Monash Center, in Prato, Italy, September 4-6, 2013. The meeting served as the final review of the EU-FP7 Instability and Control of Massively Separated Flows Marie Curie travel grant and was supported by the European Office of Aerospace Research and Development. Fifty leading specialists from twelve countries reviewed the progress made since the 50s of the last century and discussed modern analysis techniques, advanced experimental flow diagnostics, and recent developments in active flow control techniques from the incompressible to the hypersonic regime. Applications involving massive flow separation and associated instability and noise generation mechanisms of interest to the aeronautical, naval and automotive industries have been addressed from a theoretical, numerical or experimental point of view, making this book a unique source containing the stat...

  19. Response of axisymmetric separated flow to its spatially localized perturbation

    Science.gov (United States)

    Dovgal, A. V.; Zanin, B. Yu.; Sorokin, A. M.

    2016-11-01

    The flow past an axisymmetric body with laminar boundary-layer separation in a low-velocity air stream has been studied. The hot-wire technique was employed to identify the variation of velocity field induced by a local stationary perturbation of separation region at the stern of the experimental model. A large-scale influence upon the near-wall flow due to a cylinder roughness element provided on the model surface was observed. The obtained data substantiate the possibility of controlling the laminar boundary-layer separation on an axisymmetric body using a local external forcing.

  20. Applications of URANS on predicting unsteady turbulent separated flows

    Institute of Scientific and Technical Information of China (English)

    Jinglei Xu; Huiyang Ma

    2009-01-01

    Accurate prediction of unsteady separated turbu-lent flows remains one of the toughest tasks and a practi-cal challenge for turbulence modeling. In this paper, a 2D flow past a circular cylinder at Reynolds number 3,900 is numerically investigated by using the technique of unsteady RANS (URANS). Some typical linear and nonlinear eddy viscosity turbulence models (LEVM and NLEVM) and a quadratic explicit algebraic stress model (EASM) are evalu-ated. Numerical results have shown that a high-performance cubic NLEVM, such as CLS, are superior to the others in simulating turbulent separated flows with unsteady vortex shedding.

  1. Applications of URANS on predicting unsteady turbulent separated flows

    Science.gov (United States)

    Xu, Jinglei; Ma, Huiyang

    2009-06-01

    Accurate prediction of unsteady separated turbulent flows remains one of the toughest tasks and a practical challenge for turbulence modeling. In this paper, a 2D flow past a circular cylinder at Reynolds number 3,900 is numerically investigated by using the technique of unsteady RANS (URANS). Some typical linear and nonlinear eddy viscosity turbulence models (LEVM and NLEVM) and a quadratic explicit algebraic stress model (EASM) are evaluated. Numerical results have shown that a high-performance cubic NLEVM, such as CLS, are superior to the others in simulating turbulent separated flows with unsteady vortex shedding.

  2. Roughness induced flow separation in adverse pressure gradient

    Science.gov (United States)

    Joo, Jongwook; Emory, Mike; Bose, Sanjeeb; Medic, Gorazd; Sharma, Om

    2016-11-01

    Surface roughness does not only increase turbulent mixing, but also thickens boundary-layers, making flows more susceptible to separation. Detailed flow physics related to the separation is not understood well. Bammert and Milsch (1972) demonstrates a clear example of surface roughness induced separation under adverse pressure gradient. In the study, compressor cascades with NACA 65 airfoils are systematically roughened and the flow over suction surface gradually separates early as roughness increases. A set of Large-Eddy Simulations (LES) over the Bammert's case is investigated, since RANS simulations using roughness models suffer from capturing the separation. In the current study, surface roughness is represented in two different approaches; 1) Realistic rough surface represented by stochastically distributed hills and valleys are gridded and solved with unstructured finite volume method, 2) Using block-structured grid, surface roughness is gridded as a staggered array of 3D rectangles, in a similar way of the previous study for roughened low pressure turbine (GT2016-57912). The current LES's capture rich features of the flow phenomena, which will bring comprehensive understanding of the roughness induced separation. This collaboration is made through 2016 CTR Summer Program.

  3. FLUID FLOW SEPARATION CHARACTER ON NOVEL HYBRID JOURNAL BEARING

    Institute of Scientific and Technical Information of China (English)

    CHEN Shujiang; LU Changhou; LI Lei

    2006-01-01

    The influence of the structure and running parameters of a novel spiral oil wedge hybrid journal bearing on the fluid flow trace is investigated. The governing equation of the flow trace of lubricant is set up, and the simulation is carried out by using finite difference method. The results show that the lubricant flow status and end leakage quantity are greatly influenced by spiral angle,and that the rotating speed has little influence on the flow status. With advisable geometry design,the separation of lubricant between different oil wedges can be obtained, which can decrease the temperature rise effectively.

  4. Microporous separators for Fe/V redox flow batteries

    Science.gov (United States)

    Wei, Xiaoliang; Li, Liyu; Luo, Qingtao; Nie, Zimin; Wang, Wei; Li, Bin; Xia, Guan-Guang; Miller, Eric; Chambers, Jeff; Yang, Zhenguo

    2012-11-01

    The Fe/V redox flow battery has demonstrated promising performance with distinct advantages over other redox flow battery systems. Due to the less oxidative nature of the Fe(III) species, hydrocarbon-based ion exchange membranes or separators can be used. Daramic® microporous polyethylene separators were tested on Fe/V flow cells using sulphuric/chloric mixed acid-supporting electrolytes. Among them, separator C exhibited good flow cell cycling performance with satisfactory repeatability over a broad temperature range of 5-50 °C. Energy efficiency (EE) of C remains around 70% at current densities of 50-80 mA cm-2 in temperatures ranging from room temperature to 50 °C. The capacity decay problem could be circumvented through hydraulic pressure balancing by means of applying different pump rates to the positive and negative electrolytes. Stable capacity and energy were obtained over 20 cycles at room temperature and 40 °C. These results show that extremely low-cost separators ($1-20 m-2) are applicable in the Fe/V flow battery system with acceptable energy efficiency. This represents a remarkable breakthrough: a significant reduction of the capital cost of the Fe/V flow battery system, which could further its market penetration in grid stabilization and renewable integration.

  5. Passive Flow Separation Control Mechanism Inspired by Shark Skin

    Science.gov (United States)

    Oakley, India; Lang, Amy

    2015-11-01

    The following experimental work seeks to examine shark scales as passive flow-actuated separation control mechanisms. It is hypothesized that the actuation of these scales can in fact reduce pressure drag by inhibiting flow reversal and thereby prevent flow separation. In order to examine this mechanism at a fundamental level, three-dimensional sharkskin scales were simplified and modeled as two-dimensional flaps. To further simplify the experiment, the flaps were observed within a laminar boundary layer. The laminar boundary layer was grown over a long flat plate that was placed inside a water tunnel. A rotating cylinder was also used to induce an unsteady, increasing adverse pressure gradient, which generated a reversing flow. In order to visualize the potential actuation of the two-dimensional flaps DPIV (digital particle image velocimetry) was utilized. Three main objectives for this work included, the actuation of the two-dimensional flaps, the resistance to a reversed flow as a result of flap actuation and the prevention of flow separation. However once the experiment was conducted the flaps did not perform as previously hypothesized. The adverse pressure gradient induced by the rotating cylinder did not produce a reversing flow powerful enough to actuate the flaps. NSF REU Site Award 1358991.

  6. Continuous-flow sheathless diamagnetic particle separation in ferrofluids

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, Yilong [Department of Mechanical Engineering, Clemson University, Clemson, SC 29634-0921 (United States); Song, Le [School of Instrument Science and Opto-electronic Engineering, Hefei University of Technology, Hefei 230009 (China); Yu, Liandong, E-mail: liandongyu@hfut.edu.cn [School of Instrument Science and Opto-electronic Engineering, Hefei University of Technology, Hefei 230009 (China); Xuan, Xiangchun, E-mail: xcxuan@clemson.edu [Department of Mechanical Engineering, Clemson University, Clemson, SC 29634-0921 (United States)

    2016-08-15

    Separating particles from a complex mixture is often necessary in many chemical and biomedical applications. This work presents a continuous-flow sheathless diamagnetic particle separation in ferrofluids through U-shaped microchannels. Due to the action of a size-dependent magnetic force, diamagnetic particles are focused into a single stream in the inlet branch of the U-turn and then continuously separated into two streams in its outlet branch. A 3D numerical model is developed to predict and understand the diamagnetic particle transport during this separation process. The numerical predictions are found to agree well with the experimental observations in a systematic study of the effects of multiple parameters including ferrofluid flow rate, concentration and magnet-channel distance. Additional numerical studies of the geometric effects of the U-turn reveal that increasing the outlet-branch width of the U-turn can significantly enhance the diamagnetic particle separation in ferrofluids. - Highlights: • Particles are focused and separated in the two branches of a U-shaped microchannel. • Negative magnetophoretic motion in ferrofluids causes the particle deflection. • A 3D numerical model is developed to simulate the particle separation. • Multiple parametric effects are studied both experimentally and numerically. • Increasing the outlet-branch width significantly enhances the particle separation.

  7. Estimates of Flow Duration, Mean Flow, and Peak-Discharge Frequency Values for Kansas Stream Locations

    Science.gov (United States)

    Perry, Charles A.; Wolock, David M.; Artman, Joshua C.

    2004-01-01

    Streamflow statistics of flow duration and peak-discharge frequency were estimated for 4,771 individual locations on streams listed on the 1999 Kansas Surface Water Register. These statistics included the flow-duration values of 90, 75, 50, 25, and 10 percent, as well as the mean flow value. Peak-discharge frequency values were estimated for the 2-, 5-, 10-, 25-, 50-, and 100-year floods. Least-squares multiple regression techniques were used, along with Tobit analyses, to develop equations for estimating flow-duration values of 90, 75, 50, 25, and 10 percent and the mean flow for uncontrolled flow stream locations. The contributing-drainage areas of 149 U.S. Geological Survey streamflow-gaging stations in Kansas and parts of surrounding States that had flow uncontrolled by Federal reservoirs and used in the regression analyses ranged from 2.06 to 12,004 square miles. Logarithmic transformations of climatic and basin data were performed to yield the best linear relation for developing equations to compute flow durations and mean flow. In the regression analyses, the significant climatic and basin characteristics, in order of importance, were contributing-drainage area, mean annual precipitation, mean basin permeability, and mean basin slope. The analyses yielded a model standard error of prediction range of 0.43 logarithmic units for the 90-percent duration analysis to 0.15 logarithmic units for the 10-percent duration analysis. The model standard error of prediction was 0.14 logarithmic units for the mean flow. Regression equations used to estimate peak-discharge frequency values were obtained from a previous report, and estimates for the 2-, 5-, 10-, 25-, 50-, and 100-year floods were determined for this report. The regression equations and an interpolation procedure were used to compute flow durations, mean flow, and estimates of peak-discharge frequency for locations along uncontrolled flow streams on the 1999 Kansas Surface Water Register. Flow durations, mean

  8. Separation of Particles in Swirling Flow in Coaxial Channel

    Directory of Open Access Journals (Sweden)

    Vasilevsky Michail

    2016-01-01

    Full Text Available Cyclones are widely used devices to separate a dispersed phase (e.g. particles or droplets from a continuous phase. The separation of particles in coaxial channels with different length is considered in paper. In this study we show that as coaxial channels length grows, the efficiency increases. In addition we demonstrate that as a gap between cylinder components is reduced, the aerosol spray efficiency is reduced also in turbulent flow.

  9. Integral methods for shallow free-surface flows with separation

    DEFF Research Database (Denmark)

    Watanabe, S.; Putkaradze, V.; Bohr, Tomas

    2003-01-01

    eddy and separated flow. Assuming a variable radial velocity profile as in Karman-Pohlhausen's method, we obtain a system of two ordinary differential equations for stationary states that can smoothly go through the jump. Solutions of the system are in good agreement with experiments. For the flow down......, and stationary jumps, obtained, for instance, behind a sluice gate. We then include time dependence in the model to study the stability of these waves. This allows us to distinguish between sub- and supercritical flows by calculating dispersion relations for wavelengths of the order of the width of the layer.......We study laminar thin film flows with large distortions of the free surface, using the method of averaging across the flow. Two specific problems are studied: the circular hydraulic jump and the flow down an inclined plane. For the circular hydraulic jump our method is able to handle an internal...

  10. Characterization of the Flow Separation of a Variable Camber Airfoil

    Institute of Scientific and Technical Information of China (English)

    YANG Wen-Chao; WANG Hui; YANG Jian-Ting; YANG Ji-Ming

    2012-01-01

    An experimental investigation is carried out to study the How separation behaviors of a variable camber airfoil. The aerodynamic load measurements and related flow visualization show that there are two types of stalls caused by the deformation on the camber: the leading-edge stall and the trailing-edge stall. Static measurements of aerodynamic force show a drastic leading-edge stall, while the serial measurements on an airfoil with camber deformation illustrate a trailing-edge stall and gradual bending-over on the aerodynamic coefficient curve. Under flow separation circumstances, the Bow structure is related not only to current boundary conditions, but also the previous flow characteristics, so the quasi-steady aerodynamic characteristics are significantly distinct from those of the static measurements.%An experimental investigation is carried out to study the flow separation behaviors of a variable camber airfoil.The aerodynamic load measurements and related flow visualization show that there are two types of stalls caused by the deformation on the camber:the leading-edge stall and the trailing-edge stall.Static measurements of aerodynamic force show a drastic leading-edge stall,while the serial measurements on an airfoil with camber deformation illustrate a trailing-edge stall and gradual bending-over on the aerodynamic coefficient curve.Under flow separation circumstances,the flow structure is related not only to current boundary conditions,but also the previous flow characteristics,so the quasi-steady aerodynamic characteristics are significantly distinct from those of the static measurements.

  11. Two-Dimensional Turbulent Separated Flow. Volume 1

    Science.gov (United States)

    1985-06-01

    of detached turbulent boundary layers, even when the sign of U is changed to account for mean backflows. Thus, earlier researchers, such as Kuhn and...Turbulent Shear Layer," Third Symposium on Turbulent Shear Flows, pp. 16.23-16.29. Hillier, R., Latour , M.E.M.P., and Cherry, N.J. (1983), "Unsteady...344. Kuhn , G.D. and Nielsen, J.N. (1971), "An Analytical Method for Calculating Turbulent Separated Flows Due to Adverse Pressure Gradients

  12. Prediction of Separation Length of Turbulent Multiphase Flow Using Radiotracer and Computational Fluid Dynamics Simulation

    Directory of Open Access Journals (Sweden)

    S. Sugiharto1

    2013-04-01

    Full Text Available Multiphase flow modeling presents great challenges due to its extreme importance in various industrial and environmental applications. In the present study, prediction of separation length of multiphase flow is examined experimentally by injection of two kinds of iodine-based radiotracer solutions into a hydrocarbon transport pipeline (HCT having an inner diameter of 24 in (60,96 m. The main components of fluids in the pipeline are water 95%, crude oil 3% and gas 2%. A radiotracing experiment was carried out at the segment of pipe which is located far from branch points with assumptions that stratified flows in such segment were achieved. Two radiation detectors located at 80 and 100 m from injection point were used to generate residence time distribution (RTD curve resulting from injection of radiotracer solutions. Multiphase computational fluid dynamics (CFD simulations using Eulerian-Eulerian control volume and commercial CFD package Fluent 6.2 were employed to simulate separation length of multiphase flow. The results of study shows that the flow velocity of water is higher than the flow rate of crude oil in water-dominated system despite the higher density of water than the density of the crude oil. The separation length in multiphase flow predicted by Fluent mixture model is approximately 20 m, measured from injection point. This result confirms that the placement of the first radiation detector at the distance 80 m from the injection point was correct

  13. Wind-Induced Ventilation Based on the Separated Flow Region

    Directory of Open Access Journals (Sweden)

    Mostafa Rahimi

    2016-01-01

    Full Text Available An experimental investigation was conducted to study the potential use of the pressure reduction within the separated flow region followed by the wake at the leeward direction of a solid surface in natural ventilation of buildings. Air flow with mean velocity up to 7 m/s was directed onto a solid surface (circular plate and a semi-spherical surface behind which the top end of a vertical vent pipe had been placed. Pressure reduction at the exit section of the pipe, which was well inside the separated flow region, induced an air flow within the pipe. This air flow rate from the stagnant surroundings into the wake region was measured under different geometrical configurations and for various wind velocities. The study revealed that the pressure reduction within the separated flow region would be applicable for natural ventilation of different spaces at least as an auxiliary system. The spaces include; sanitary places, crop protection stocks, industrial workshops and other spaces where no regular ventilation is required.

  14. Feedback Control of Flow Separation Using Plasma Actuator and FBG Sensor

    Directory of Open Access Journals (Sweden)

    Takehiko Segawa

    2016-01-01

    Full Text Available A feedback control system for mitigating flow separation was developed by using a string-type dielectric-barrier-discharge (DBD plasma actuator and a fiber Bragg grating (FBG sensor. Tangential jets were induced from the string-type DBD plasma actuator, which was located at 5% chord from the leading edge of an NACA0024 airfoil. The FBG sensor was attached to the interior surface near the root of the cantilever beam modeled on the pressure surface of the airfoil. The strain at the cantilever root was reflected in the form of Bragg wavelengths (λB detected by the FBG sensor when the cantilever tip was vibrated by the flow near the trailing edge of the airfoil. It was found that calculating running standard deviations in the Bragg wavelength (λB′ detected by the sensor was valuable for judging flow separation in real time. The feedback control of flow separation on the NACA0024 airfoil was successfully demonstrated by setting λB′=0.0028 with periodic flow separations generated in a wind tunnel by oscillating a side wall of the test section with frequency fw=0.42 Hz. It was confirmed that the appearance probability of flow separation tends to decrease with a decrease in the duration for calculating λB′ and with an increase in the duration of jet injection.

  15. Flow Rate of He Ⅱ Liquid-Vapor Phase Separator

    Institute of Scientific and Technical Information of China (English)

    Xingen YU; Qing LI; Qiang LI; Zhengyu LI

    2005-01-01

    Experimental results are presented for superfluld (He Ⅱ) flow through porous plug liquid-vapor phase separators.Tests have been performed on seven porous plugs with different thicknesses or different permeabilities. The temperature was measured from 1.5K to 1.9K. Two flow regions were observed in small and large pressure and temperature differences regions respectively. The experimental data are compared with theoretical predictions.The performance and applicability of the basic theory are discussed. Hysteresis of the flow rate is also observed and discussed.

  16. Separated flows near the nose of a body of revolution

    Science.gov (United States)

    Lin, S. P.

    1986-01-01

    The solution of the Navier-Stokes equations for the problem of cross-flow separataion about a deforming cylinder was achieved by iteration. It was shown that the separation starts at the rear stagnation point and the point of primary separation moves upstram along the cylinder surface. A general method of linear stability analysis for nonparallel external flows was constructed, which consists of representing the eigenfunctions with complete orthogonal sets and forms characteristic equations with the Galerkin method. The method was applied to the Kovasznay flow which is an exact solution of the Navier-Stokes equation. The results show that when the critical parameter is exceeded, there are only a few isolated unstable eigen-frequencies. Another exact solution is shown to be absolutely and monotonically stable with respect to infinitesimal disturbances of all frequencies. The flow is also globally, asymptotically, and monotonically stable in the mean with respect o three-dimensional disturbances. This result forms the sound foundation of rigorous stability analysis for nonparallel flows, and provides an invaluable test ground for future studies of nonparallel flows in which the basic states do not posses exact solutions. The application of this method to the study of the formation of spiral vorticies near the nose of a rotating body of revolution is underway. The same method will be applied to the stability analysis of reversed flow over a plate with suction.

  17. Exploring active flow control for efficient control of separation on an Ahmed model

    Science.gov (United States)

    McNally, Jonathan; Alvi, Farrukh

    2016-11-01

    Active flow control is applied to an Ahmed model with a rear slant angle of 25°, where a typical flow field consists of a three-dimensional separation region on the rear slant of the bluff body. Linear arrays of discrete microjets, previously proven to effectively control this separation, are investigated further. A principal aim of this experimental study is to examine the sensitivity of control as the actuator location is shifted with respect to the separation location. Aerodynamic force and surface pressure measurements, combined with the velocity field obtained using particle image velocimetry, provide a measure of control efficacy and insight into the interaction of jet arrays with the local flow field, including the separating shear layer. An energy balance is conducted to characterize control efficiency for multiple positions over a range of microjet array blowing conditions. Results show that moving the actuator array further into the separation region requires higher microjet momentum to obtain a desired aerodynamic benefit. An empirical relationship is also developed for determining the required jet velocity as a function of position by relating the jet penetration distance to local flow features and length scales. Partial support by FCAAP and NSF.

  18. SIMULATION OF THE PROCESS OF FLOW SEPARATION AT THE ENTRANCE OF SQUARE ASPIRATING PORT

    Directory of Open Access Journals (Sweden)

    Olga A. Averkova

    2014-01-01

    Full Text Available We consider the flow at the inlet to the suction square hole with sharp edges, which is located in an infinite space. The purpose of this study is to construct a mathematical model of flow separation at the entrance to square suction canal with sharp edges, located in infinite space, by using square vortex frameworks. As a part of ideology of the method of discrete vortices in the non-stationary quasi-axisymmetric formulation, we constructed the mathematical model of separated flow at the inlet to the square aspirating pipe and its software-algorithmic implementation. We have determined the velocity field at the entrance to suction channel and a line of flow separation. Determine the velocity field in typical cross-sections of the suction channel, dimensions of the efficient absorption, compression ratio of the jet. Were received analytical formulas for calculating separation surfaces current. Profiling the entrance opening of the suction hole detected on the outlines separation surface will improve the acoustic and aerodynamic properties of the exhaust systems. The obtained results can be useful for designing of local exhaust ventilation of reduced energy consumption.

  19. Phase separating colloid polymer mixtures in shear flow

    NARCIS (Netherlands)

    Derks, D.; Aarts, D.G.A.L.; Bonn, D.; Imhof, A.

    2008-01-01

    We study the process of phase separation of colloid polymer mixtures in the (spinodal) two-phase region of the phase diagram in shear flow. We use a counter-rotating shear cell and image the system by means of confocal laser scanning microscopy. The system is quenched from an initially almost homoge

  20. Flow Separation Control on Airfoil With Pulsed Nanosecond Discharge Actuator

    NARCIS (Netherlands)

    Correale, G.; Popov, I.B.; Ratikin, A.E.; Starikovskii, A.Y.; Hulshoff, S.J.; Veldhuis, L.L.M.

    2011-01-01

    An experimental study of flow separation control with a nanosecond pulse plasma actuator was performed in wind-tunnel experiments. The discharge used had a pulse width of 12 ns and rising time of 3 ns with voltage up to 12 kV. Repetition frequency was adjustable up to 10 kHz. The first series of exp

  1. General Separations Area (GSA) Groundwater Flow Model Update: Hydrostratigraphic Data

    Energy Technology Data Exchange (ETDEWEB)

    Bagwell, L. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Bennett, P. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Flach, G. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2017-02-21

    This document describes the assembly, selection, and interpretation of hydrostratigraphic data for input to an updated groundwater flow model for the General Separations Area (GSA; Figure 1) at the Department of Energy’s (DOE) Savannah River Site (SRS). This report is one of several discrete but interrelated tasks that support development of an updated groundwater model (Bagwell and Flach, 2016).

  2. Phase separating colloid polymer mixtures in shear flow

    NARCIS (Netherlands)

    Derks, D.; Aarts, D.; Bonn, D.; Imhof, A.

    2008-01-01

    We study the process of phase separation of colloid polymer mixtures in the (spinodal) two-phase region of the phase diagram in shear flow. We use a counter-rotating shear cell and image the system by means of confocal laser scanning microscopy. The system is quenched from an initially almost

  3. WEAKLY SWIRLING TURBULENT FLOW IN TURBID WATER HYDRAULIC SEPARATION DEVICE

    Institute of Scientific and Technical Information of China (English)

    LI Lin; QIU Xiu-yun; JIN Sheng; XIAO Jun; GONG Shou-yuan

    2008-01-01

    This article deals with the characteristics of weakly swirling turbulent flow field in a Turbid Water Hydraulic Separation Device (TWHSD) through experimental and numerical researches. The flow field was measured by PIV, which provided streamlines, vortex structure, vorticity and velocity distribution in different test planes in the TWHSD. On the basis of the experimental results, the tangential and radial velocity distributions of the swirling flow field were obtained. Meanwhile, the numerical simulations were conducted with the RNG and RSM turbulence models, respectively. According to the experimental and numerical results, the characteristics of the clear water flow field inside the TWHSD were determined. In view of simulation accuracy and time consumption, it is suggested to apply the RNG model instead of the RSM model, which is more time consuming, to make further study on two-phases flow fields in the device.

  4. Injection Induced Mixing in Flows Separating From Smooth Surfaces

    Science.gov (United States)

    Adamczyk, John J. (Technical Monitor); Wundrow, David W.

    2004-01-01

    An analytic model for predicting the effect of unsteady local surface injection on the flow separating from a streamlined body at angle of attack is proposed. The model uses the premise that separation control results from enhanced mixing along the shear layer that develops between the main stream and the fluid in the underlying recirculation zone. High-Reynolds-number asymptotic methods are used to connect the unsteady surface injection to an instability wave propagating on the separating shear layer and then to the large-scale coherent structures that produce the increased mixing. The results is a tool that can guide the choice of fluid-actuator parameters to maximize flow-control effectiveness and may also facilitate computer-based numerical experiments.

  5. An inviscid model of flow separation around blunt bodies

    Science.gov (United States)

    Khristov, C. I.; Todorov, M. D.

    The theoretical model developed by Christov and Todorov (1984) to describe separation in inviscid flows around blunt bodies is extended and refined, with an emphasis on the numerical implementation of the model. The two-dimensional steady flow around an infinite cylinder of starlike cross section is analyzed in polar coordinates, assuming the flow to be symmetric with respect to a plane containing the central axis of the cylinder, and the stagnation zones are treated using parabolic coordinates. Consideration is given to the coordinate transformation and the difference schemes for the Laplace equation and the equation for stagnation-zone shape. Numerical results are presented graphically and briefly characterized. It is found that separation is essentially an inviscid phenomenon, but triggered by viscosity; except for the skin friction, the resistance can be accurately assessed using the inviscid model.

  6. A Novel Method for Separating and Locating Multiple Partial Discharge Sources in a Substation.

    Science.gov (United States)

    Li, Pengfei; Zhou, Wenjun; Yang, Shuai; Liu, Yushun; Tian, Yan; Wang, Yong

    2017-01-27

    To separate and locate multi-partial discharge (PD) sources in a substation, the use of spectrum differences of ultra-high frequency signals radiated from various sources as characteristic parameters has been previously reported. However, the separation success rate was poor when signal-to-noise ratio was low, and the localization result was a coordinate on two-dimensional plane. In this paper, a novel method is proposed to improve the separation rate and the localization accuracy. A directional measuring platform is built using two directional antennas. The time delay (TD) of the signals captured by the antennas is calculated, and TD sequences are obtained by rotating the platform at different angles. The sequences are separated with the TD distribution feature, and the directions of the multi-PD sources are calculated. The PD sources are located by directions using the error probability method. To verify the method, a simulated model with three PD sources was established by XFdtd. Simulation results show that the separation rate is increased from 71% to 95% compared with the previous method, and an accurate three-dimensional localization result was obtained. A field test with two PD sources was carried out, and the sources were separated and located accurately by the proposed method.

  7. Simulation of turbulent flow and temperature separation in a uni-flow vortex tube

    Directory of Open Access Journals (Sweden)

    Promvonge, P.

    2007-03-01

    Full Text Available The vortex tube is a mechanical device operating as a refrigerating machine without refrigerants, by separating a compressed gas stream into two streams; the cold air stream at the tube core while the hot airstream near the tube wall. Such a separation of the flow into regions of low and high total temperature is referred to as the temperature separation effect. In this paper, simulation of the turbulent compressible flowand temperature separation in a uni-flow vortex tube with the turbulence model and the algebraic Reynolds stress model (ASM is described. Steady, compressible and two-dimensional flows are assumed through outthe calculation. It has been found that the predicted results of velocity, pressure, and temperature fields are generally in good agreement with available experiment data. Moreover, it can be indicated that the highest temperature separation occurs near the inlet nozzle while the lowest temperature separation is found at the downstream near the control valve.

  8. Manipulating flow separation: sensitivity of stagnation points, separatrix angles and recirculation area to steady actuation

    CERN Document Server

    Boujo, Edouard

    2014-01-01

    A variational technique is used to derive analytical expressions for the sensitivity of several geometric indicators of flow separation to steady actuation. Considering the boundary layer flow above a wall-mounted bump, the six following representative quantities are considered: the locations of the separation point and reattachment point connected by the separatrix, the separation angles at these stagnation points, the backflow area and the recirculation area. For each geometric quantity, linear sensitivity analysis allows us to identify regions which are the most sensitive to volume forcing and wall blowing/suction. Validations against full non-linear Navier-Stokes calculations show excellent agreement for small-amplitude control for all considered indicators. With very resemblant sensitivity maps, the reattachment point, the backflow and recirculation areas are seen to be easily manipulated. In contrast, the upstream separation point and the separatrix angles are seen to remain extremely robust with respec...

  9. Geometrical effects on the airfoil flow separation and transition

    KAUST Repository

    Zhang, Wei

    2015-04-25

    We present results from direct numerical simulations (DNS) of incompressible flow over two airfoils, NACA-4412 and NACA-0012-64, to investigate the effects of the airfoil geometry on the flow separation and transition patterns at Re=104 and 10 degrees incidence. The two chosen airfoils are geometrically similar except for maximum camber (respectively 4%C and 0 with C the chord length), which results in a larger projection area with respect to the incoming flow for the NACA-4412 airfoil, and a larger leeward surface curvature at the leading edge for the NACA-0012-64 airfoil. The governing equations are discretized using an energy conservative fourth-order spatial discretization scheme. An assessment on the two-point correlation indicates that a spanwise domain size of 0.8C is sufficiently large for the present simulations. We discuss flow separation at the airfoil leading edge, transition of the separated shear layer to three-dimensional flow and subsequently to turbulence. Numerical results reveal a stronger adverse pressure gradient field in the leading edge region of the NACA-0012-64 airfoil due to the rapidly varying surface curvature. As a result, the flow experiences detachment at x/C=0.08, and the separated shear layer transition via Kelvin-Helmholtz mechanism occurs at x/C=0.29 with fully developed turbulent flow around x/C=0.80. These flow development phases are delayed to occur at much downstream positions, respectively, observed around x/C=0.25, 0.71 and 1.15 for the NACA-4412 airfoil. The turbulent intensity, measured by the turbulent fluctuations and turbulent Reynolds stresses, are much larger for NACA-0012-64 from the transition onset until the airfoil trailing edge, while turbulence develops significantly downstream of the trailing edge for the NACA-4412 airfoil. For both airfoils, our DNS results indicate that the mean Reynolds stress u\\'u\\'/U02 reaches its maximum value at a distance from the surface approximately equal to the displacement

  10. Sources of resonant sound in separated duct flows

    Science.gov (United States)

    Hourigan, K.; Welsh, M. C.; Thompson, M. C.; Stokes, A. N.

    An overview of studies at the Commonwealth Scientific and Industrial Research Organization of Australia involving the generation of sound in separating flows observed in wind tunnels is presented. Numerical experiments to pinpoint the sources of sound in these flows are reported. It is found that resonant sound can be sustained by the correct phasing of sound power generation by a vortex with the resonant acoustic field. A feedback loop is established whereby the vortex shedding itself is locked to the sound field. For a given acoustic mode and frequency, the existence of vortex shedding correctly phased to generate net acoustic power is a function of the flow velocity; acoustic resonance then appears periodically as the flow velocity is increased.

  11. Numerical simulation of flow separation control by oscillatory fluid injection

    Science.gov (United States)

    Resendiz Rosas, Celerino

    2005-07-01

    In this work, numerical simulations of flow separation control are performed. The separation control technique studied is called "synthetic jet actuation". The developed code employs a cell centered finite volume scheme which handles viscous, steady and unsteady compressible turbulent flows. The pulsating zero mass jet flow is simulated by imposing a harmonically varying transpiration boundary condition on the airfoil's surface. Turbulence is modeled with the algebraic model of Baldwin and Lomax. The application of synthetic jet actuators is based in their ability to energize the boundary layer, thereby providing significant increase in the lift coefficient. This has been corroborated experimentally and it is corroborated numerically in this research. The performed numerical simulation investigates the flow over a NACA0015 airfoil. For this flow Re = 9 x 105 and the reduced frequency and momentum coefficient are F + = 1.1 and Cmu = 0.04 respectively. The oscillatory injection takes place at 12.27% chord from the leading edge. A maximum increase in the mean lift coefficient of 93% is predicted by the code. A discrepancy of approximately 10% is observed with corresponding experimental data from the literature. The general trend is, however, well captured. The discrepancy is attributed to the modeling of the injection boundary condition and to the turbulence model. A sensitivity analysis of the lift coefficient to different values of the oscillation parameters is performed. It is concluded that tangential injection, F+ ≈ O(1) and the utilized grid resolution around the site of injection are optimal. Streamline fields obtained for different angles of injection are analyzed. Flow separation and attachment as functions of the injection angle and of the velocity of injection can be observed. It is finally concluded that a reliable numerical tool has been developed which can be utilized as a support tool in the optimization of the synthetic jet operation and in the

  12. Optimal Control of Airfoil Flow Separation using Fluidic Excitation

    Science.gov (United States)

    Shahrabi, Arireza F.

    This thesis deals with the control of flow separation around a symmetric airfoils with the aid of multiple synthetic jet actuators (SJAs). CFD simulation methods have been implemented to uncover the flow separation regimes and associated properties such as frequencies and momentum ratio. In the first part of the study, the SJA was studied thoroughly. Large Eddy Simulations (LES) were performed for one individual cavity; the time history of SJA of the outlet velocity profile and the net momentum imparted to the flow were analyzed. The studied SJA is asymmetrical and operates with the aid of a piezoelectric (PZT) ceramic circular plate actuator. A three-dimensional mesh for the computational domain of the SJA and the surrounding volume was developed and was used to evaluate the details of the airflow conditions inside the SJA as well as at the outlet. The vibration of the PZT ceramic actuator was used as a boundary condition in the computational model to drive the SJA. Particular attention was given to developing a predictive model of the SJA outlet velocity. Results showed that the SJA velocity output is correlated to the PZT ceramic plate vibration, especially for the first frequency mode. SJAs are a particular class of zero net mass flux (ZNMF) fluidic devices with net imparted momentum to the flow. The net momentum imparted to the flow in the separated region is such that positive enhancement during AFC operations is achieved. Flows around the NACA 0015 airfoil were simulated for a range of operating conditions. Attention was given to the active open and closed loop control solutions for an airfoil with SJA at different angles of attack and flap angles. A large number of simulations using RANS & LES models were performed to study the effects of the momentum ratio (Cμ) in the range of 0 to 11% and of the non-dimensional frequency, F+, in the range of 0 to 2 for the control of flow separation at a practical angle of attack and flap angle. The optimum value of C

  13. Dynamical separation of spherical bodies in supersonic flow

    OpenAIRE

    Laurence, Stuart; Parziale, N. J.; Deiterding, Ralf

    2012-01-01

    An experimental and computational investigation of the unsteady separation behaviour of two spheres in a highly supersonic flow is carried out. The spherical bodies, initially touching, are released with negligible relative velocity, an arrangement representing the idealized binary fragmentation of a meteoritic body in the atmosphere. In experiments performed in a Mach-4 Ludwieg tube, nylon spheres are initially suspended in the test section by weak threads and, following detachment of ...

  14. Separation of microscale chiral objects by shear flow

    OpenAIRE

    Marcos,, Tavares; Fu, Henry C.; Powers, Thomas R.; Stocker, Roman

    2010-01-01

    We show that plane parabolic flow in a microfluidic channel causes nonmotile helically-shaped bacteria to drift perpendicular to the shear plane. Net drift results from the preferential alignment of helices with streamlines, with a direction that depends on the chirality of the helix and the sign of the shear rate. The drift is in good agreement with a model based on resistive force theory, and separation is efficient (>80%) and fast (

  15. Chiral Separation by Flows: The Role of Flow Symmetry and Dimensionality

    Science.gov (United States)

    Ro, Sunghan; Yi, Juyeon; Kim, Yong Woon

    2016-01-01

    Separation of enantiomers by flows is a promising chiral resolution method using cost-effective microfluidics. Notwithstanding a number of experimental and numerical studies, a fundamental understanding still remains elusive, and an important question as to whether it is possible to specify common physical properties of flows that induce separation has not been addressed. Here, we study the separation of rigid chiral objects of an arbitrary shape induced by a linear flow field at low Reynolds numbers. Based on a symmetry property under parity inversion, we show that the rate-of-strain field is essential to drift the objects in opposite directions according to chirality. From eigenmode analysis, we also derive an analytic expression for the separation conditions which shows that the flow field should be quasi-two-dimensional for the precise and efficient resolutions of microscopic enantiomers. We demonstrate this prediction by Langevin dynamics simulations with hydrodynamic interactions fully implemented. Finally, we discuss the practical feasibility of the linear flow analysis, considering separations by a vortex flow or an extensional flow under a confining potential. PMID:27739430

  16. Numerical Simulations of Separated Flows Using Wall-Modeled LES

    Science.gov (United States)

    Vane, Zachary; Ortega, Jason; Salari, Kambiz

    2014-11-01

    Calculations using an unstructured, wall-modeled large eddy simulation (WMLES) solver are performed for several high Reynolds number test cases of interest. While the equilibrium formulation of this wall-model (Bodart, Larsson & Moin, AIAA 2013-2724) has proven to be accurate for steady, attached boundary layers, its application to non-equilibrium or highly three-dimensional problems has yet to be fully explored. A series of turbulent flows that exhibit boundary layer separation due to the geometries involved in each test case are considered. First, spanwise-periodic simulations for the flow over periodic hills are performed at multiple Reynolds numbers. Next, calculations involving separation caused by three-dimensional bodies are used to generate more complex flow fields and to evaluate the accuracy of the WMLES in the separated wake region downstream. The performance of the WMLES is quantified through comparisons with existing numerical and experimental data sets. The effects of grid resolution and variations in several wall-model parameters are also investigated to determine their influence on the overall calculation.

  17. Efficient solution of two-dimensional steady separated flows

    Science.gov (United States)

    Napolitano, M.

    This work is concerned with the numerical solution of 2D incompressible steady laminar separated flows at moderate-to-high values of Re. The vorticity-stream function Navier-Stokes equations, as well as approximate models based upon the boundary-layer theory, will be considered. The main objective of the paper is to present the development of an efficient approach for solving a class of problems usually referred to as high Re weakly separated flows. A description is given of a block-alternating-direction-implicit method, which applies the approximate factorization scheme of Beam and Warming to the vorticity-stream function equations, using the delta form of the deferred correction procedure of Khosla and Rubin to combine the stability of upwind schemes with the accuracy of central differences. The logical steps which led to the development of a more efficient incremental block-line Gauss-Seidel method and to a simple multigrid strategy particularly suited for this kind of numerical scheme are then outlined. Finally, benchmark-quality solutions for separated flows inside diffusers and channels with smooth as well as sudden expansions are presented.

  18. Integral methods for shallow free-surface flows with separation

    CERN Document Server

    Watanabe, S; Bohr, T; Watanabe, Shinya; Putkaradze, Vachtang; Bohr, Tomas

    2000-01-01

    We study laminar thin film flows with large distortions in the free surface using the method of averaging across the flow. Two concrete problems are studied: the circular hydraulic jump and the flow down an inclined plane. For the circular hydraulic jump our method is able to handle an internal eddy and separated flow. Assuming a variable radial velocity profile like in Karman-Pohlhausen's method, we obtain a system of two ordinary differential equations for stationary states that can smoothly go through the jump where previous studies encountered a singularity. Solutions of the system are in good agreement with experiments. For the flow down an inclined plane we take a similar approach and derive a simple model in which the velocity profile is not restricted to a parabolic or self-similar form. Two types of solutions with large surface distortions are found: solitary, kink-like propagating fronts, obtained when the flow rate is suddenly changed, and stationary jumps, obtained, e.g., behind a sluice gate. We ...

  19. Nanoparticle separation with a miniaturized asymmetrical flow field-flow fractionation cartridge

    Directory of Open Access Journals (Sweden)

    David eMüller

    2015-07-01

    Full Text Available Asymmetrical Flow Field-Flow Fractionation (AF4 is a separation technique applicable to particles over a wide size range. Despite the many advantages of AF4, its adoption in routine particle analysis is somewhat limited by the large footprint of currently available separation cartridges, extended analysis times and significant solvent consumption. To address these issues, we describe the fabrication and characterization of miniaturized AF4 cartridges. Key features of the scale-down platform include simplified cartridge and reagent handling, reduced analysis costs and higher throughput capacities. The separation performance of the miniaturized cartridge is assessed using certified gold and silver nanoparticle standards. Analysis of gold nanoparticle populations indicates shorter analysis times and increased sensitivity compared to conventional AF4 separation schemes. Moreover, nanoparticulate titanium dioxide populations exhibiting broad size distributions are analyzed in a rapid and efficient manner. Finally, the repeatability and reproducibility of the miniaturized platform are investigated with respect to analysis time and separation efficiency.

  20. Parametric Studies of Flow Separation using Air Injection

    Science.gov (United States)

    Zhang, Wei

    2004-01-01

    Boundary Layer separation causes the airfoil to stall and therefore imposes dramatic performance degradation on the airfoil. In recent years, flow separation control has been one of the active research areas in the field of aerodynamics due to its promising performance improvements on the lifting device. These active flow separation control techniques include steady and unsteady air injection as well as suction on the airfoil surface etc. This paper will be focusing on the steady and unsteady air injection on the airfoil. Although wind tunnel experiments revealed that the performance improvements on the airfoil using injection techniques, the details of how the key variables such as air injection slot geometry and air injection angle etc impact the effectiveness of flow separation control via air injection has not been studied. A parametric study of both steady and unsteady air injection active flow control will be the main objective for this summer. For steady injection, the key variables include the slot geometry, orientation, spacing, air injection velocity as well as the injection angle. For unsteady injection, the injection frequency will also be investigated. Key metrics such as lift coefficient, drag coefficient, total pressure loss and total injection mass will be used to measure the effectiveness of the control technique. A design of experiments using the Box-Behnken Design is set up in order to determine how each of the variables affects each of the key metrics. Design of experiment is used so that the number of experimental runs will be at minimum and still be able to predict which variables are the key contributors to the responses. The experiments will then be conducted in the 1ft by 1ft wind tunnel according to the design of experiment settings. The data obtained from the experiments will be imported into JMP, statistical software, to generate sets of response surface equations which represent the statistical empirical model for each of the metrics as

  1. Active Flow Control and Global Stability Analysis of Separated Flow Over a NACA 0012 Airfoil

    Science.gov (United States)

    Munday, Phillip M.

    The objective of this computational study is to examine and quantify the influence of fundamental flow control inputs in suppressing flow separation over a canonical airfoil. Most flow control studies to this date have relied on the development of actuator technology, and described the control input based on specific actuators. Taking advantage of a computational framework, we generalize the inputs to fundamental perturbations without restricting inputs to a particular actuator. Utilizing this viewpoint, generalized control inputs aim to aid in the quantification and support the design of separation control techniques. This study in particular independently introduces wall-normal momentum and angular momentum to the separated flow using swirling jets through model boundary conditions. The response of the flow field and the surface vorticity fluxes to various combinations of actuation inputs are examined in detail. By closely studying different variables, the influence of the wall-normal and angular momentum injections on separated flow is identified. As an example, open-loop control of fully separated, incompressible flow over a NACA 0012 airfoil at alpha = 6° and 9° with Re = 23,000 is examined with large-eddy simulations. For the shallow angle of attack alpha = 6°, the small recirculation region is primarily affected by wall-normal momentum injection. For a larger separation region at alpha = 9°, it is observed that the addition of angular momentum input to wall-normal momentum injection enhances the suppression of flow separation. Reducing the size of the separated flow region significantly impacts the forces, and in particular reduces drag and increases lift on the airfoil. It was found that the influence of flow control on the small recirculation region (alpha = 6°) can be sufficiently quantified with the traditional coefficient of momentum. At alpha = 9°, the effects of wall-normal and angular momentum inputs are captured by modifying the standard

  2. Characterization and reduction of flow separation in jet pumps for laminar oscillatory flows

    CERN Document Server

    Timmer, Michael A G; Bühler, Simon; Wilcox, Douglas; van der Meer, Theo H

    2016-01-01

    A computational fluid dynamics model is used to predict the oscillatory flow through tapered cylindrical tube sections (jet pumps). The asymmetric shape of jet pumps results in a time-averaged pressure drop that can be used to suppress Gedeon streaming in closed-loop thermoacoustic devices. However, previous work has shown that flow separation in the diverging flow direction counteracts the time-averaged pressure drop. In this work, the characteristics of flow separation in jet pumps are identified and coupled with the observed jet pump performance. Furthermore, it is shown that the onset of flow separation can be shifted to larger displacement amplitudes by designs that have a smoother transition between the small opening and the tapered surface of the jet pump. These design alterations also reduce the duration of separated flow, resulting in more effective and robust jet pumps. To make the proposed jet pump designs more compact without reducing their performance, the minimum big opening radius that can be i...

  3. Multiple sample flow through immunomagnetic separator for concentrating pathogenic bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Rotariu, Ovidiu [School of Biological Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen (United Kingdom); Ogden, Iain D [Department of Medical Microbiology, University of Aberdeen, Aberdeen (United Kingdom); MacRae, Marion [Department of Medical Microbiology, University of Aberdeen, Aberdeen (United Kingdom); Udrea, Laura Elena [National Institute of R-D for Technical Physics I.F.T. Iasi, Mangeron 47 Blvd., Iasi (Romania); Strachan, Norval J C [School of Biological Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen (United Kingdom)

    2005-06-21

    The standard method of immunomagnetic separation for isolating pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (<0.5 pathogenic bacteria/g) will not be consistently detected by this method. Here a multiple sample flow through immunomagnetic separator has been designed and tested to process large volume samples (50 to 250 ml). Preliminary results show >97% recovery of polydisperse magnetic particles (diameter range 1 to 8 {mu}m) containing 29-33% w/w Fe{sub 3}O{sub 4} content. Between 70 and 130 times more of the pathogenic bacteria Escherichia coli O157 is recovered from PBS compared with the standard 1 ml method. Also, the recovery of E. coli O157 from beef mince homogenates, after a 4 h incubation at 42 deg. C, is between 80 and 180 times higher than the standard 1 ml method.

  4. Protein separation by continuous-flow electrophoresis in microgravity.

    Science.gov (United States)

    Clifton, M J; Roux-de Balmann, H; Sanchez, V

    1996-07-01

    During the IML-2 space shuttle mission, the RAMSES instrument was operated in the Spacelab module. This continuous-flow electrophoresis device performs separation and purification of protein solutions on a preparative scale. Samples containing artificial mixtures of pure proteins were used to test the capabilities of the device, and useful separations were obtained for proteins having a mobility difference of only 3 x 10(-9) m2 V-1 s-1. Operating conditions that cannot be applied on earth were explored for two different sample concentrations, one of which is too high to allow treatment on earth. It agrees well with a previously published numerical model in that the main cause of loss in resolution in this process is the electrohydrodynamic spreading of the protein filaments.

  5. Unsteady flow past wings having sharp-edge separation

    Science.gov (United States)

    Atta, E. H.; Kandil, O. A.; Mook, D. T.; Nayfeh, A. H.

    1976-01-01

    A vortex-lattice technique is developed to model unsteady, incompressible flow past thin wings. This technique predicts the shape of the wake as a function of time; thus, it is not restricted by planform, aspect ratio, or angle of attack as long as vortex bursting does not occur and the flow does not separate from the wing surface. Moreover, the technique can be applied to wings of arbitrary curvature undergoing general motion; thus, it can treat rigid-body motion, arbitrary wing deformation, gusts in the freestream, and periodic motions. Numerical results are presented for low-aspect rectangular wings undergoing a constant-rate, rigid-body rotation about the trailing edge. The results for the unsteady motion are compared with those predicted by assuming quasi-steady motion. The present results exhibit hysteretic behavior.

  6. The Effects of Sweeping Jet Actuator Parameters on Flow Separation Control

    Science.gov (United States)

    Koklu, Mehti

    2015-01-01

    A parametric experimental study was performed with sweeping jet actuators (fluidic oscillators) to determine their effectiveness in controlling flow separation on an adverse pressure gradient ramp. Actuator parameters that were investigated include blowing coefficients, operation mode, pitch and spreading angles, streamwise location, aspect ratio, and scale. Surface pressure measurements and surface oil flow visualization were used to characterize the effects of these parameters on the actuator performance. 2D Particle Image Velocimetry measurements of the flow field over the ramp and hot-wire measurements of the actuator's jet flow were also obtained for selective cases. In addition, the sweeping jet actuators were compared to other well-known flow control techniques such as micro-vortex generators, steady blowing, and steady vortex-generating jets. The results confirm that the sweeping jet actuators are more effective than steady blowing and steady vortex-generating jets. The results also suggest that an actuator with a larger spreading angle placed closer to the location where the flow separates provides better performance. For the cases tested, an actuator with an aspect ratio, which is the width/depth of the actuator throat, of 2 was found to be optimal. For a fixed momentum coefficient, decreasing the aspect ratio to 1 produced weaker vortices while increasing the aspect ratio to 4 reduced coverage area. Although scaling down the actuator (based on the throat dimensions) from 0.25 inch x 0.125 inch to 0.15 inch x 0.075 inch resulted in similar flow control performance, scaling down the actuator further to 0.075 inch x 0.0375 inch reduced the actuator efficiency by reducing the coverage area and the amount of mixing in the near-wall region. The results of this study provide insight that can be used to design and select the optimal sweeping jet actuator configuration for flow control applications.

  7. An ID/Locator Separation Based Group Mobility Management in Wireless Body Area Network

    Directory of Open Access Journals (Sweden)

    Moneeb Gohar

    2015-01-01

    Full Text Available Mobility management in wireless sensor network is the most important factor to be considered for applications such as healthcare system. Recently, Identifier (ID/Locator (LOC separation based mobility management scheme has been proposed for wireless sensor network. However, it does not perform well in group-based mobility management in wireless body area network, and thus it tends to induce large registration, packet delivery, and handover delays. To overcome these limitations, we propose a group-based mobility management scheme based on ID/LOC separation concept for ID-based communications with location-based routing to reduce the number of control messages. In the proposed scheme, each sensor device has a globally unique device identifier (GDID which contains the information of its home network domain. For handover support, each access gateway maintains its home GDID register (HGR and visiting GDID register (VGR which are used to keep the GDID-locator (LOC mappings for primary mobile devices in the distributed manner. Besides, in the proposed scheme, only the coordinator will send Router Solicitation and Router Advertisement messages to reduce the control messages further. By numerical analysis, we show that the proposed scheme can significantly reduce the registration, packet delivery, and handover delays, compared to the existing schemes.

  8. Mechanism governing separation in microfluidic pinched flow fractionation devices

    CERN Document Server

    Risbud, Sumedh R

    2014-01-01

    We present a computational investigation of the mechanism governing size-based particle separation in microfluidic pinched flow fractionation. We study the behavior of particles moving through a pinching gap (i.e., a constriction in the aperture of a channel) in the Stokes regime as a function of particle size. The constriction aperture is created by a plane wall and spherical obstacle, and emulates the pinching segment in pinched flow fractionation devices. The simulation results show that the distance of closest approach between the particle and obstacle surfaces (along a trajectory) decreases with increasing particle size. We then use the distance of closest approach to investigate the effect of short-range repulsive non-hydrodynamic interactions (e.g., solid-solid contact). We define a critical trajectory as the one in which the minimum particle-obstacle separation is equal to the range of the non-hydrodynamic interactions. The results further show that the initial offset of the critical trajectory (defin...

  9. Large-Flow-Area Flow-Selective Liquid/Gas Separator

    Science.gov (United States)

    Vasquez, Arturo; Bradley, Karla F.

    2010-01-01

    This liquid/gas separator provides the basis for a first stage of a fuel cell product water/oxygen gas phase separator. It can separate liquid and gas in bulk in multiple gravity environments. The system separates fuel cell product water entrained with circulating oxygen gas from the outlet of a fuel cell stack before allowing the gas to return to the fuel cell stack inlet. Additional makeup oxygen gas is added either before or after the separator to account for the gas consumed in the fuel cell power plant. A large volume is provided upstream of porous material in the separator to allow for the collection of water that does not exit the separator with the outgoing oxygen gas. The water then can be removed as it continues to collect, so that the accumulation of water does not impede the separating action of the device. The system is designed with a series of tubes of the porous material configured into a shell-and-tube heat exchanger configuration. The two-phase fluid stream to be separated enters the shell-side portion of the device. Gas flows to the center passages of the tubes through the porous material and is then routed to a common volume at the end of the tubes by simple pressure difference from a pumping device. Gas flows through the porous material of the tubes with greater ease as a function of the ratio of the dynamic viscosity of the water and gas. By careful selection of the dimensions of the tubes (wall thickness, porosity, diameter, length of the tubes, number of the tubes, and tube-to-tube spacing in the shell volume) a suitable design can be made to match the magnitude of water and gas flow, developed pressures from the oxygen reactant pumping device, and required residual water inventory for the shellside volume.

  10. Separation of bacterial spores from flowing water in macro-scale cavities by ultrasonic standing waves

    CERN Document Server

    Lipkens, B; Costolo, M; Stevens, A; Rietman, Edward

    2010-01-01

    The separation of micron-sized bacterial spores (Bacillus cereus) from a steady flow of water through the use of ultrasonic standing waves is demonstrated. An ultrasonic resonator with cross-section of 0.0254 m x 0.0254 m has been designed with a flow inlet and outlet for a water stream that ensures laminar flow conditions into and out of the resonator section of the flow tube. A 0.01905-m diameter PZT-4, nominal 2-MHz transducer is used to generate ultrasonic standing waves in the resonator. The acoustic resonator is 0.0356 m from transducer face to the opposite reflector wall with the acoustic field in a direction orthogonal to the water flow direction. At fixed frequency excitation, spores are concentrated at the stable locations of the acoustic radiation force and trapped in the resonator region. The effect of the transducer voltage and frequency on the efficiency of spore capture in the resonator has been investigated. Successful separation of B. cereus spores from water with typical volume flow rates of...

  11. Vocal Fold Pathologies and Three-Dimensional Flow Separation Phenomena

    Science.gov (United States)

    Apostoli, Adam G.; Weiland, Kelley S.; Plesniak, Michael W.

    2013-11-01

    Polyps and nodules are two different pathologies, which are geometric abnormalities that form on the medial surface of the vocal folds, and have been shown to significantly disrupt a person's ability to communicate. Although the mechanism by which the vocal folds self-oscillate and the three-dimensional nature of the glottal jet has been studied, the effect of irregularities caused by pathologies is not fully understood. Examining the formation and evolution of vortical structures created by a geometric protuberance is important, not only for understanding the aerodynamic forces exerted by these structures on the vocal folds, but also in the treatment of the above-mentioned pathological conditions. Using a wall-mounted prolate hemispheroid with a 2:1 aspect ratio in cross flow, the present investigation considers three-dimensional flow separation induced by a model vocal fold polyp. Building on previous work using skin friction line visualization, both the velocity flow field and wall pressure measurements around the model polyp are presented and compared. Supported by the National Science Foundation, Grant No. CBET-1236351 and GW Center for Biomimetics and Bioinspired Engineering (COBRE).

  12. Active flow management in preparative chromatographic separations: a preliminary investigation into enhanced separation using a curtain flow inlet fitting and segmented flow outlet fitting.

    Science.gov (United States)

    Camenzuli, Michelle; Ritchie, Harald J; Ladine, James R; Shalliker, R Andrew

    2012-02-01

    Active flow management in the form of curtain flow sample introduction and segmented outlet flow control has been shown to enable sample to elute through a chromatography column under the principles of the "infinite diameter column". Such an elution process avoids the detrimental effects of the heterogeneity of particle-packed chromatographic columns by injecting the sample directly into the radial core region of the column, thus avoiding wall effects. The process described herein illustrates how the principles of the infinite diameter column can be applied using conventional injection devices suitable for long-term analysis that requires robust protocols. Using this approach, sensitivity in separation was 2.5 times greater than conventional chromatography, yielding a product at twice the concentration. Benefits of curtain flow injection are thus relevant to both preparative-scale and analytical-scale separations.

  13. Comparison of plateletpheresis on three continuous flow cell separators

    Directory of Open Access Journals (Sweden)

    Tendulkar Anita

    2009-01-01

    Full Text Available Introduction: Platelet concentrate (PC remains one of the most important support measures in thrombocytopenic patients. An efficient cell separator is a prerequisite for an optimally functioning apheresis setup. Donor blood count may undergo a temporary reduction after the procedure. Aim: The aim was to find the extent of reduction in donor blood count (hemoglobin, hematocrit, white blood cell, and platelet after plateletpheresis and to evaluate the cell separator for collection efficiency, processing time, and leukoreduction. Study Design and Methods: Two hundred and thirty seven procedures performed on the Amicus (N = 121, Fenwal CS-3000 Plus (N = 50 and Cobe spectra (N = 66 in a one year period were evaluated. The procedures performed on the continuous flow centrifugation (CFC cell separators and donor blood counts (pre and post donation done were included in the study. Results: The percent reduction in hemoglobin (HB, hematocrit (HCT, white blood cell (WBC and platelet count ((PLT ct was 2.9, 3.1, 9, 30.7 (Mean, N = 237 respectively after the procedure. The post donation PLT ct reduced to < 100x109/L (range 80-100 in five donors (N = 5/237, Amicus. The pre donation PLT ct in them was 150-200x109/L. Collection efficiency (percent of Amicus (79.3 was better as compared to the other two machines (CS: 62.5, Cobe: 57.5. PC collected on Cobe spectra had < 1x106 WBC. The donor pre donation PLT levels had a positive correlation to the product PLT yield (r = 0.30, P = 0.000. Conclusion: Monitoring donor blood counts helps to avoid pheresis induced adverse events. A cautious approach is necessary in donors whose pre donation PLT ct is 150-200x109/L. The main variable in PLT yield is donor PLT ct (pre donation. High collection efficiency is a direct measure of an optimally functioning cell separator.

  14. A continuous flow micro filtration device for plasma/blood separation using submicron vertical pillar gap structures

    Science.gov (United States)

    Kang, Tae Goo; Yoon, Yong-Jin; Ji, Hongmiao; Lim, Pei Yi; Chen, Yu

    2014-08-01

    This work demonstrates a continuous flow plasma/blood separator using a vertical submicron pillar gap structure. The working principle of the proposed separator is based on size exclusion of cells through cross-flow filtration, in which only plasma is allowed to pass through submicron vertical pillars located tangential to the main flow path of the blood sample. The maximum filtration efficiency of 99.9% was recorded with a plasma collection rate of 0.67 µl min-1 for an input blood flow rate of 12.5 µl min-1. The hemolysis phenomenon was observed for an input blood flow rate above 30 µl min-1. Based on the experimental results, we can conclude that the proposed device shows potential for the application of on-chip plasma/blood separation as a part of integrated point-of-care (POC) diagnostics systems.

  15. Mitigation of shock-induced flow separation using magnetohydrodynamic flow control

    Indian Academy of Sciences (India)

    R BALASUBRAMANIAN; K ANANDHANARAYANAN; R KRISHNAMURTHY; DEBASIS CHAKRABORTY

    2017-03-01

    A numerical investigation is carried out to demonstrate a proof of concept, magnetohydrodynamicsbased active flow control, for mitigation of laminar flow separation over a flat plate due to shock wave–boundary layer interaction. The CERANS-MHD code has been used to solve the governing resistive magnetohydrodynamic equations discretized in finite-volume framework. The AUSM-PW? flux function is used in modellingthe advection terms and central differencing is used in modelling the resistive terms. Powell’s source term method is used for divergence cleaning of the magnetic field. The Hartmann number is varied from 0 to 12,000 to effectuate mitigation of flow separation, with the magnetic field applied at the wall and oriented transverse to the flat plate flow direction. Due to the Hartmann effect, flow separation is observed to be suppressed withincrease in Hartmann number beyond 6000. However, the overall magnitude of skin friction distribution increases drastically, resulting in large increase in skin friction drag as compared with the non-magnetic case,and is a cause of concern.

  16. Location of South Georgia and potential impact on early Pacific-Atlantic through flow

    Science.gov (United States)

    Carter, A.; Curtis, M.

    2013-12-01

    One of the most significant Cenozoic reconfigurations of global ocean circulation involved the initiation of Pacific to Atlantic exchange that led to the isolation of Antarctica by the Antarctica Circumpolar Current though the separation of South America and Antarctica and the opening of the Scotia Sea and Drake Passage. Whether significant Pacific to Atlantic through-flow was possible in the early Cenozoic has remained unclear because it is not certain where continental fragments such as South Georgia, a potential barrier, were located before seafloor spreading created the Scotia Sea. Establishing where South Georgia was located is also critical to reconstructing the Scotia arc and understanding its evolution. Detrital zircon U-Pb geochronology and apatite thermochronometry are used to constrain the pre-drift location of South Georgia. Data from Cretaceous turbidites exposed on South Georgia are consistent with a former connection to the Rocas Verdes back-arc basin giving support to models that have argued for a pre- tectonic translation location southeast of Tierra del Fuego. Following an early phase of rock uplift, thermal history models of the apatite chronometry data indicate that the South Georgia continental fragment underwent burial related heating and was therefore not a significant topographic feature until it emerged c. 10-7 Ma coeval with the cessation of spreading at the West Scotia Ridge and collision between the South Georgia continental block and the Northeast Georgia Rise.

  17. Detection of flow separation and stagnation points using artificial hair sensors

    Science.gov (United States)

    Phillips, D. M.; Ray, C. W.; Hagen, B. J.; Su, W.; Baur, J. W.; Reich, G. W.

    2015-11-01

    Recent interest in fly-by-feel approaches for aircraft control has motivated the development of novel sensors for use in aerial systems. Artificial hair sensors (AHSs) are one type of device that promise to fill a unique niche in the sensory suite for aerial systems. In this work, we investigate the capability of an AHS based on structural glass fibers to directly identify flow stagnation and separation points on a cylindrical domain in a steady flow. The glass fibers are functionalized with a radially aligned carbon nanotube (CNT) forest and elicit a piezoresistive response as the CNT forest impinges on electrodes in a micropore when the hair is deflected due to viscous drag forces. Particle image velocimetry is used to measure the flow field allowing for the resulting moment and force acting on the hair to be correlated with the electrical response. It is demonstrated that the AHS provides estimates for the locations of both the stagnation and separation in steady flow. From this, a simulation of a heading estimation is presented to demonstrate a potential application for hair sensors. These results motivate the construction of large arrays of hair sensors for imaging and resolving flow structures in real time.

  18. Propagations of fluctuations and flow separation on an unsteadily loaded airfoil

    Science.gov (United States)

    Tenney, Andrew; Lewalle, Jacques

    2014-11-01

    We analyze pressure data from 18 taps located along the surface of a DU-96-W180 airfoil in bothand steady flow conditions. The conditions were set to mimic the flow conditions experienced by a wind turbine blade under unsteady loading to test and to quantify the effects of several flow control schemes. Here we are interested in the propagation of fluctuations along the pressure and suction sides, particularly in relation to the fluctuating separation point. An unsteady phase of the incoming fluctuations is defined using Morlet wavelets, and phase-conditioned cross-correlations are calculated. Using wavelet-based pattern recognition, individual events in the pressure data are identified with several different algorithms utilizing both the original time series pressure signals and their corresponding scalograms. The data analyzed in this study was collected by G. Wang in the Skytop anechoic chamber at Syracuse University in the spring of 2013; the work of Zhe Bai on this data is also acknowledged.

  19. Shooter position estimation with muzzle blast and shockwave measurements from separate locations

    Science.gov (United States)

    Grasing, David

    2016-05-01

    There are two acoustical events associated with small arms fire: the muzzle blast (created by bullets being expelled from the barrel of the weapon), and the shockwave (created by bullets which exceed the speed of sound). Assuming the ballistics of a round are known, the times and directions of arrival of the acoustic events furnish sufficient information to determine the origin of the shot. Existing methods tacitly assume that it is a single sensor which makes measurements of the times and direction of arrival. If the sensor is located past the point where the bullet goes transonic or if the sensor is far off the axis of the shot line a single sensor localization become highly inaccurate due to the ill-conditioning of the localization problem. In this paper, a more general approach is taken which allows for localizations from measurements made at separate locations. There are considerable advantages to this approach, the most noteworthy of which is the improvement in localization accuracy due to the improvement in the conditioning of the problem. Additional benefits include: the potential to locate in cases where a single sensor has insufficient information, furnishing high quality initialization to data fusion algorithms, and the potential to identify the round from a set of possible rounds.

  20. Phase separating colloid polymer mixtures in shear flow

    Energy Technology Data Exchange (ETDEWEB)

    Derks, Didi; Imhof, Arnout [Soft Condensed Matter, Debye Institute, Utrecht University, Princetonplein 5, 3584 CC Utrecht (Netherlands); Aarts, Dirk G A L [Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford OX1 3QZ (United Kingdom); Bonn, Daniel [Laboratoire de Physique Statistique, Ecole Normale Superieure, 24 rue Lhomond, 75231 Paris cedex 05 (France)], E-mail: didi.derks@lps.ens.fr

    2008-10-08

    We study the process of phase separation of colloid polymer mixtures in the (spinodal) two-phase region of the phase diagram in shear flow. We use a counter-rotating shear cell and image the system by means of confocal laser scanning microscopy. The system is quenched from an initially almost homogeneous state at very high (200 s{sup -1}) shear rate to a low shear rate {gamma}-dot. A spinodal decomposition pattern is observed. Initially, the characteristic length scale increases linearly with time. As the structure coarsens, the shear imposes a certain length scale on the structure and a clear asymmetry develops. The domains become highly stretched along the flow direction, and the domain width along the vorticity axis reaches a stationary size, which scales as approx. {gamma}-do{sup -0.35}. Furthermore, on quenching from an intermediate (6.7 s{sup -1}) to a low shear rate the elongated structures become Rayleigh unstable and break up into smaller droplets. Still, the system eventually reaches the same steady state as was found from a direct high to low shear rate quench through coarsening.

  1. In-Line Oil-Water Separation in Swirling Flow (USB stick)

    NARCIS (Netherlands)

    Slot, J.J.; van Campen, L.J.A.M.; Hoeijmakers, Hendrik Willem Marie; Mudde, R.F.; Johansen, S.T.

    2011-01-01

    An in-line oil-water separator has been designed and is investigated for single- and two-phase flow. Numerical single-phase flow results show an annular reversed flow region. This flow pattern agrees qualitatively with results from measurements. In the two-phase flow simulations two different drag

  2. On-chip free-flow magnetophoresis: Separation and detection of mixtures of magnetic particles in continuous flow

    NARCIS (Netherlands)

    Pamme, Nicole; Eijkel, Jan C.T.; Manz, Andreas

    2006-01-01

    The complete separation of mixtures of magnetic particles was achieved by on-chip free-flow magnetophoresis. In continuous flow, magnetic particles were deflected from the direction of larninar flow by a perpendicular magnetic field depending on their magnetic susceptibility and size and on the flow

  3. Numerical Prediction of Entropy Generation in Separated Flows

    Directory of Open Access Journals (Sweden)

    Eiyad Abu-Nada

    2005-10-01

    Full Text Available Abstract: The present research investigates second law analysis of laminar flow over a backward facing step (BFS. Entropy generation due to separation, reattachment, recirculation and heat transfer is studied numerically. Local entropy generation distributions were obtained by solving momentum, energy, and entropy generation equations. The effect of dimensionless temperature difference number (τ and Brinkman number (Br on the total entropy generation number (Ns was investigated. Moreover, the effect of Reynolds number (Re on the value of Ns was reported. It was found that as Re increased the value of Ns increased. Also, as Br increased the value of Ns increased. However, it was found that as τ increased the value of Ns decreased. For the bottom wall of the channel, the maximum value of Ns occurs inside the recirculation zone and reduces to a minimum value at the point of reattachment point. Also, for Re ≥ 500, a second peak of entropy generation appears after the reattachment point. For the top wall of the channel, the value of Ns has a maximum value directly above the step and its value reduced downstream the step. The contribution of the top wall to Ns downstream the point of reattachment was relatively small.

  4. Directional gene flow and ecological separation in Yersinia enterocolitica

    Science.gov (United States)

    Reuter, Sandra; Corander, Jukka; de Been, Mark; Harris, Simon; Cheng, Lu; Hall, Miquette; Thomson, Nicholas R.

    2015-01-01

    Yersinia enterocolitica is a common cause of food-borne gastroenteritis worldwide. Recent work defining the phylogeny of the genus Yersinia subdivided Y. enterocolitica into six distinct phylogroups. Here, we provide detailed analyses of the evolutionary processes leading to the emergence of these phylogroups. The dominant phylogroups isolated from human infections, PG3–5, show very little diversity at the sequence level, but do present marked patterns of gain and loss of functions, including those involved in pathogenicity and metabolism, including the acquisition of phylogroup-specific O-antigen loci. We tracked gene flow across the species in the core and accessory genome, and show that the non-pathogenic PG1 strains act as a reservoir for diversity, frequently acting as donors in recombination events. Analysis of the core and accessory genome also suggested that the different Y. enterocolitica phylogroups may be ecologically separated, in contrast to the long-held belief of common shared ecological niches across the Y. enterocolitica species.

  5. Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow-through separation of beads and cells.

    Science.gov (United States)

    Wang, Lisen; Lu, Jente; Marchenko, Steven A; Monuki, Edwin S; Flanagan, Lisa A; Lee, Abraham P

    2009-03-01

    This paper presents a novel design and separation strategy for lateral flow-through separation of cells/particles in microfluidics by dual frequency coupled dielectrophoresis (DEP) forces enabled by vertical interdigitated electrodes embedded in the channel sidewalls. Unlike field-flow-fractionation-DEP separations in microfluidics, which utilize planar electrodes on the microchannel floor to generate a DEP force to balance the gravitational force and separate objects at different height locations, lateral separation is enabled by sidewall interdigitated electrodes that are used to generate non-uniform electric fields and balanced DEP forces along the width of the microchannel. In the current design, two separate AC electric fields are applied to two sets of independent interdigitated electrode arrays fabricated in the sidewalls of the microchannel to generate differential DEP forces that act on the cells/particles flowing through. Individual particles (cells or beads) will experience DEP forces differently due to the difference in their dielectric properties. The balance of the differential DEP forces from the electrode arrays will position dissimilar particles at distinct equilibrium planes across the width of the channel. When coupled with fluid flow, this results in lateral separation along the width of the microchannel and the separated particles can thus be automatically directed into branched channel outlets leading to different reservoirs for downstream processing. In this paper, we present the design and analysis of lateral separation enabled by dual frequency coupled DEP, and cell/bead and cell/cell separations are demonstrated with this lateral separation strategy. With vertical interdigitated electrodes on the sidewall, the height of the microchannel can be increased without losing the electric field strength in contrast to other multiple frequency DEP devices with planar electrodes. As a result, populations of cells can be separated simultaneously

  6. Limiting flows of a viscous fluid with stationary separation zones with Re approaching infinity

    Science.gov (United States)

    Taganov, G. I.

    1982-01-01

    The limiting flows of a viscous noncondensable fluid, which are approached by flows with stationary separation zones behind planar symmetrical bodies, with an unlimited increase in the Reynolds number are studied. Quantitative results are obtained in the case of a circulation flow inside of a separation zone.

  7. Modelling of two-phase flow based on separation of the flow according to velocity

    Energy Technology Data Exchange (ETDEWEB)

    Narumo, T. [VTT Energy, Espoo (Finland). Nuclear Energy

    1997-12-31

    The thesis concentrates on the development work of a physical one-dimensional two-fluid model that is based on Separation of the Flow According to Velocity (SFAV). The conventional way to model one-dimensional two-phase flow is to derive conservation equations for mass, momentum and energy over the regions occupied by the phases. In the SFAV approach, the two-phase mixture is divided into two subflows, with as distinct average velocities as possible, and momentum conservation equations are derived over their domains. Mass and energy conservation are treated equally with the conventional model because they are distributed very accurately according to the phases, but momentum fluctuations follow better the flow velocity. Submodels for non-uniform transverse profile of velocity and density, slip between the phases within each subflow and turbulence between the subflows have been derived. The model system is hyperbolic in any sensible flow conditions over the whole range of void fraction. Thus, it can be solved with accurate numerical methods utilizing the characteristics. The characteristics agree well with the used experimental data on two-phase flow wave phenomena Furthermore, the characteristics of the SFAV model are as well in accordance with their physical counterparts as of the best virtual-mass models that are typically optimized for special flow regimes like bubbly flow. The SFAV model has proved to be applicable in describing two-phase flow physically correctly because both the dynamics and steady-state behaviour of the model has been considered and found to agree well with experimental data This makes the SFAV model especially suitable for the calculation of fast transients, taking place in versatile form e.g. in nuclear reactors. 45 refs. The thesis includes also five previous publications by author.

  8. Investigation of flow separation in a transonic-fan linear cascade using visualization methods

    Energy Technology Data Exchange (ETDEWEB)

    Lepicovsky, J. [ASRC Aerospace Corporation, NASA GRC, Cleveland, OH (United States)

    2008-06-15

    An extensive experimental study into the nature of the separated flows on the blade suction surface of modern transonic fans is described in this paper. The study was a subtask of a larger experimental effort focused on blade flutter excited by flow separation in the blade tip region. The tip sections of airfoils on transonic fan blades are designed for precompression and consequently they differ from sections on the rest of the blade. The blade tip section was modeled by a low aspect ratio blade and therefore most of the blade tested was exposed to the secondary flow effects. The aim of this work was to supply reliable data on flow separation on transonic fan blades for validation of future analytical studies. The experimental study focused on two visualization techniques: surface flow visualization using dye oils and schlieren (and shadowgraph) flow visualization. The following key observations were made during the study. For subsonic inlet flow, the flow on the suction surface of the blade was separated over a large portion of the blade, and the separated area increased with increasing inlet Mach number. For the supersonic inlet flow condition, the flow was attached from the leading edge up to the point where a bow shock from the upper neighboring blade imposed on the blade surface. Downstream, there was a separated flow region in which air flowed in the direction opposite the inlet flow. Finally, past the separated flow region, the flow reattached to the blade surface. For subsonic inlet flow, the low cascade solidity resulted in an increased area of separated flow. For supersonic flow conditions, the low solidity resulted in an improvement in flow over the suction surface. (orig.)

  9. Investigation of flow separation in a transonic-fan linear cascade using visualization methods

    Science.gov (United States)

    Lepicovsky, J.

    2008-06-01

    An extensive experimental study into the nature of the separated flows on the blade suction surface of modern transonic fans is described in this paper. The study was a subtask of a larger experimental effort focused on blade flutter excited by flow separation in the blade tip region. The tip sections of airfoils on transonic fan blades are designed for precompression and consequently they differ from sections on the rest of the blade. The blade tip section was modeled by a low aspect ratio blade and therefore most of the blade tested was exposed to the secondary flow effects. The aim of this work was to supply reliable data on flow separation on transonic fan blades for validation of future analytical studies. The experimental study focused on two visualization techniques: surface flow visualization using dye oils and schlieren (and shadowgraph) flow visualization. The following key observations were made during the study. For subsonic inlet flow, the flow on the suction surface of the blade was separated over a large portion of the blade, and the separated area increased with increasing inlet Mach number. For the supersonic inlet flow condition, the flow was attached from the leading edge up to the point where a bow shock from the upper neighboring blade imposed on the blade surface. Downstream, there was a separated flow region in which air flowed in the direction opposite the inlet flow. Finally, past the separated flow region, the flow reattached to the blade surface. For subsonic inlet flow, the low cascade solidity resulted in an increased area of separated flow. For supersonic flow conditions, the low solidity resulted in an improvement in flow over the suction surface.

  10. Optimal separation times for electrical field flow fractionation with Couette flows.

    Science.gov (United States)

    Pascal, Jennifer; O'Hara, Ryan; Oyanader, Mario; Arce, Pedro E

    2008-11-01

    The prediction of optimal times of separation as a function of the applied electrical field and cation valence have been studied for the case of field flow fractionation [Martin M., Giddings J. C., J. Phys. Chem. 1981, 85, 727] with charged solutes. These predictions can be very useful to a priori design or identify optimal operating conditions for a Couette-based device for field flow fractionation when the orthogonal field is an electrical field. Mathematically friendly relationships are obtained by applying the method of spatial averaging to the solute species continuity equation; this is accomplished after the role of the capillary geometrical dimensions on the applied electrical field equations has been assessed [Oyanader M. A., Arce P., Electrophoresis 2005; 26, 2857]. Moreover, explicit analytical expressions are derived for the effective parameters, i.e. diffusivity and convective velocity as functions of the applied (orthogonal) electrical field. These effective transport parameters are used to study the effect of the cation valence of the solutes and of the magnitude of the applied orthogonal electrical field on the values of the optimal time of separation. These parameters play a significant role in controlling the optimal separation time, leading to a family of minimum values, for particular magnitudes of the applied orthogonal electrical field.

  11. Computation of Particle Laden Turbulent gas Jet Flows Employing the Stochastic Separated Flow Approach

    Directory of Open Access Journals (Sweden)

    V. Ramanujachari

    1992-10-01

    Full Text Available The dispersion of particles in the presence of Turbulent gas flow is studied theoretically using a stochastic separated flow model and the results compared with the available experimental data. As the particle loading in the jet is of the order of 0.1-0.4 per cent, the particles are assumed to have negligible effect on the mean and the turbulent gas phase properties (one-way coupling. The particle-turbulent eddy interactions are calculated by paying attention to the energy containing eddies, characterised by the integral length scale. The fluctuating velocities are sampled randomly from Gaussian distribution, and the particle trajectories are obtained using a procedure similar to random-walk computation. A large number of particle trajectories are averaged to obtain the statistical nature of the turbulent gas-particle jet. It is seen that the particles with less inertia, which are characterised by the Stokes number, tend to diffuse more. The turbulent diffusivities of the particles are in agreement with the available experimental data, when the time-averaged velocities of gas and particles are the same, obtained by the stochastic separated flow model.

  12. Turbulent shear flow downstream of a sphere with and without an o-ring located over a plane boundary

    Directory of Open Access Journals (Sweden)

    Sahin Besir

    2012-04-01

    Full Text Available Flow-structure interaction of separated shear flow from the sphere and a flat plate was investigated by using dye visualization and the particle image velocimetry technique. Later, a passive control method was applied with 2mm oring located on the sphere surface at 55° from front stagnation point. The experiments were carried out in open water channel for Reynolds number value of Re=5000. Flow characteristics have been examined in terms of the 2-D instantaneous and time-averaged velocity vectors, patterns of vorticity, streamlines, rms of velocity fluctuations and Reynolds stress variations and discussed from the point of flow physics, vortex formation, lengths of large-scale Karman Vortex Streets and Kelvin-Helmholtz vortices depending on the sphere locations over the flat plate. It is demonstrated that the gap flow occurring between the sphere bottom point and the flat plate surface has very high scouring effect until h/d=0.25 and then unsymmetrical flow structure of the wake region keeps up to h/D=1.0 for smooth sphere. For the sphere with o-ring, the wake flow structure becomes symmetrical at smaller gap ratios and reattachment point on the flat plate surface occurs earlier. Moreover, o-ring on the sphere diminishes peak magnitudes of the flow characteristics and thus it is expected that the flow-induced forces will be lessened both on the sphere and flat plate surface. Vortex formation lengths and maximum value occurring points become closer locations to the rear surface of the sphere with o-ring.

  13. Turbulent shear flow downstream of a sphere with and without an o-ring located over a plane boundary

    Science.gov (United States)

    Ozgoren, Muammer; Okbaz, Abdulkerim; Dogan, Sercan; Sahin, Besir; Akilli, Huseyin

    2012-04-01

    Flow-structure interaction of separated shear flow from the sphere and a flat plate was investigated by using dye visualization and the particle image velocimetry technique. Later, a passive control method was applied with 2mm oring located on the sphere surface at 55° from front stagnation point. The experiments were carried out in open water channel for Reynolds number value of Re=5000. Flow characteristics have been examined in terms of the 2-D instantaneous and time-averaged velocity vectors, patterns of vorticity, streamlines, rms of velocity fluctuations and Reynolds stress variations and discussed from the point of flow physics, vortex formation, lengths of large-scale Karman Vortex Streets and Kelvin-Helmholtz vortices depending on the sphere locations over the flat plate. It is demonstrated that the gap flow occurring between the sphere bottom point and the flat plate surface has very high scouring effect until h/d=0.25 and then unsymmetrical flow structure of the wake region keeps up to h/D=1.0 for smooth sphere. For the sphere with o-ring, the wake flow structure becomes symmetrical at smaller gap ratios and reattachment point on the flat plate surface occurs earlier. Moreover, o-ring on the sphere diminishes peak magnitudes of the flow characteristics and thus it is expected that the flow-induced forces will be lessened both on the sphere and flat plate surface. Vortex formation lengths and maximum value occurring points become closer locations to the rear surface of the sphere with o-ring.

  14. Investigation of Flow Separation in a Transonic-fan Linear Cascade Using Visualization Methods

    Science.gov (United States)

    Lepicovsky, Jan; Chima, Rodrick V.; Jett, Thomas A.; Bencic, Timothy J.; Weiland, Kenneth E.

    2000-01-01

    An extensive study into the nature of the separated flows on the suction side of modem transonic fan airfoils at high incidence is described in the paper. Suction surface.flow separation is an important flow characteristic that may significantly contribute to stall flutter in transonic fans. Flutter in axial turbomachines is a highly undesirable and dangerous self-excited mode of blade oscillations that can result in high cycle fatigue blade failure. The study basically focused on two visualization techniques: surface flow visualization using dye oils, and schlieren (and shadowgraph) flow visualization. The following key observations were made during the study. For subsonic inlet flow, the flow on the suction side of the blade is separated over a large portion of the blade, and the separated area increases with increasing inlet Mach number. For the supersonic inlet flow condition, the flow is attached from the leading edge up to the point where a bow shock from the upper neighboring blade hits the blade surface. Low cascade solidity, for the subsonic inlet flow, results in an increased area of separated flow. For supersonic flow conditions, a low solidity results in an improvement in flow over the suction surface. Finally, computational results modeling the transonic cascade flowfield illustrate our ability to simulate these flows numerically.

  15. Open-loop control of noise amplification in a separated boundary layer flow

    CERN Document Server

    Boujo, Edouard; Gallaire, François

    2014-01-01

    Linear optimal gains are computed for the subcritical two-dimensional separated boundary-layer flow past a bump. Very large optimal gain values are found, making it possible for small-amplitude noise to be strongly amplified and to destabilize the flow. The optimal forcing is located close to the summit of the bump, while the optimal response is the largest in the shear layer. The largest amplification occurs at frequencies corresponding to eigenvalues which first become unstable at higher Reynolds number. Nonlinear direct numerical simulations show that a low level of noise is indeed sufficient to trigger random flow unsteadiness, characterized here by large-scale vortex shedding. Next, a variational technique is used to compute efficiently the sensitivity of optimal gains to steady control (through source of momentum in the flow, or blowing/suction at the wall). A systematic analysis at several frequencies identifies the bump summit as the most sensitive region for control with wall actuation. Based on thes...

  16. Separated two-phase flow regime parameter measurement by a high speed ultrasonic pulse-echo system.

    Science.gov (United States)

    Masala, Tatiana; Harvel, Glenn; Chang, Jen-Shih

    2007-11-01

    In this work, a high speed ultrasonic multitransducer pulse-echo system using a four transducer method was used for the dynamic characterization of gas-liquid two-phase separated flow regimes. The ultrasonic system consists of an ultrasonic pulse signal generator, multiplexer, 10 MHz (0.64 cm) ultrasonic transducers, and a data acquisition system. Four transducers are mounted on a horizontal 2.1 cm inner diameter circular pipe. The system uses a pulse-echo method sampled every 0.5 ms for a 1 s duration. A peak detection algorithm (the C-scan mode) is developed to extract the location of the gas-liquid interface after signal processing. Using the measured instantaneous location of the gas/liquid interface, two-phase flow interfacial parameters in separated flow regimes are determined such as liquid level and void fraction for stratified wavy and annular flow. The shape of the gas-liquid interface and, hence, the instantaneous and cross-sectional averaged void fraction is also determined. The results show that the high speed ultrasonic pulse-echo system provides accurate results for the determination of the liquid level within +/-1.5%, and the time averaged liquid level measurements performed in the present work agree within +/-10% with the theoretical models. The results also show that the time averaged void fraction measurements for a stratified smooth flow, stratified wavy flow, and annular flow qualitatively agree with the theoretical predictions.

  17. Phase separation under two-dimensional Poiseuille flow.

    Science.gov (United States)

    Kiwata, H

    2001-05-01

    The spinodal decomposition of a two-dimensional binary fluid under Poiseuille flow is studied by numerical simulation. We investigated time dependence of domain sizes in directions parallel and perpendicular to the flow. In an effective region of the flow, the power-law growth of a characteristic length in the direction parallel to the flow changes from the diffusive regime with the growth exponent alpha=1/3 to a new regime. The scaling invariance of the growth in the perpendicular direction is destroyed after the diffusive regime. A recurrent prevalence of thick and thin domains which determines log-time periodic oscillations has not been observed in our model. The growth exponents in the infinite system under two-dimensional Poiseuille flow are obtained by the renormalization group.

  18. Analysis of Interline Power Flow Controller (IPFC Location in Power Transmission Systems

    Directory of Open Access Journals (Sweden)

    Amir Kahyaei

    2011-07-01

    Full Text Available The Interline Power Flow Controller (IPFC is one of the latest generation Flexible AC Transmission Systems (FACTS controller used to control power flows of multiple transmission lines. The aim of this paper is investigation of the effect of location of IPFC on profile of voltage and real and reactive power flow in transmission lines in power system. This model is incorporated in Newton- Raphson (NR power flow algorithm to study the power flow control in transmission lines in which IPFC is placed. A program in MATLAB/SIMULINK has been written in order to extend conventional NR algorithm based on this model. Numerical results are carried out on a standard power system. The results without and with IPFC for various locations are compared in terms of voltages, active and reactive power flows to demonstrate the performance of the IPFC model.

  19. Identification of separate flow features in the shear layer

    Science.gov (United States)

    Mulleners, Karen; Krishna, Swathi; Green, Melissa

    2016-11-01

    Analyzing unsteady flow fields primarily involves the identification of dynamically significant regions of vorticity in the flow. Detection of all the flow features is essential for an accurate description of the physics of the flow, which eventually helps in improving flow modeling and predictions. Eulerian criteria such as λ2 and Γ2 successfully identify large scale structures based on local velocity gradients and topology but do not detect the coherent vortices with the concentrated vorticity in a shear layer. The identification of these smaller structures within the shear layer is important when predicting the overall circulatory contribution to the aerodynamic forces produced, in applications such as flapping wing design. In order to detect the smaller flow features along with the prominent large scale vortices, an alternative method of vortex identification is proposed in which the flow structures are detected based on the vorticity contours. This method is applied to numerical and experimental data of a pitching panel to highlight its robustness. In addition, the finite time Lyapunov exponent (FTLE) is calculated to show that the boundaries of the material lines and identified vorticity contours coincide.

  20. Microfluidic Separation of a Soluble Substance Using Transverse Diffusion in a Layered Flow

    Directory of Open Access Journals (Sweden)

    Xuan Don Nguyen

    2016-12-01

    Full Text Available This paper presents a practical flow-through method to separate anisole and ethyl phenylacetate, respectively, from a polystyrene mixture. The microfluidic separation uses different diffusive dynamics of the substances transverse to the lamination flow formed in a microchannel. The effect of inlet flow rates and ambient temperature on separation is examined. Additionally, the possibility of the separation of the light substance from the mixture with different molecular weight is shown numerically and experimentally. The separation efficiency is explained by the facts that the relaxation time depends on the inlet flow rate and that the diffusivity depends on the ambient temperature. This method can be applied to separate monomers from aggregates.

  1. Secondary flows occurring in a whirlpool separator – A study of phenomena – observation, simulation and measurements

    Directory of Open Access Journals (Sweden)

    Jakubowski Marek

    2015-09-01

    Full Text Available The whirlpool separator, used for hot trub separation, is prevalent in the brewing industry. It is a kind of a hydrocyclone inside of which a tea leaf effect occurs, which is sediment accumulation into a cone shape at the central part of the tank’s bottom. This manner of sediment accumulation is caused by the secondary flow occurring in the so-called Ekman boundary layer. This article is a summary of the research, which has been conducted for many years and involved observation, simulation and experimental research on the recognition and formation of the secondary flow accumulating the sediment cone. Secondary flows occurring in a whirlpool were identified through CFD simulation and PIV experiments, and are presented in this paper. Based on their location and direction, an attempt to determine their impact on the separation process taking place in the whirlpool has been made. The secondary flow identification methods proposed in this paper can be successfully applied in other solutions, e. g. structural ones, which involve rotational-flow-based separation.

  2. Experimental Investigation of Flow Separation Control Using Dielectric Barrier Discharge Plasma Actuators

    Institute of Scientific and Technical Information of China (English)

    LI Gang; NIE Chaoqun; LI Yiming; ZHU Junqiang; XU Yanji

    2008-01-01

    Influence of plasma actuators as a flow separation control device was investigated experimentally.Hump model was used to demonstrate the effect of plasma actuators on external flow separation,while for internal flow separation a set of compressor cascade was adopted.In order to investigate the modification of the flow structure by the plasma actuator,the flow field was examined non-intrusively by particle image velocimetry measurements in the hump model experiment and by a hot film probe in the compressor cascade experiment.The results showed that the plasma actuator could be effective in controlling the flow separation both over the hump and in the compressor cascade when the incoming velocity was low.As the incoming velocity increased,the plasma actuator was less effective. It is urgent to enhance the intensity of the plasma actuator for its better application.Methods to increase the intensity of plasma actuator were also studied.

  3. Influence of sodium chloride on shear flow induced starch-gluten separation from Soissons wheat dough

    NARCIS (Netherlands)

    Zalm, van der E.E.J.; Goot, van der A.J.; Boom, R.M.

    2010-01-01

    Wheat dough can be separated into a starch-rich and a gluten-rich fraction by subjecting the dough to curvilinear shear flow. This paper presents the effect of salt (NaCl) addition on the shear-induced separation process. The separation (defined as the changes in protein concentration in the various

  4. CFD modeling of particle behavior in supersonic flows with strong swirls for gas separation

    DEFF Research Database (Denmark)

    Yang, Yan; Wen, Chuang

    2017-01-01

    flow from the dry gas outlet. The separation efficiency reached over 80%, when the droplet diameter was more than 1.5 μm. The optimum length of the cyclonic separation section was approximate 16–20 times of the nozzle throat diameter to obtain higher collection efficiency for the supersonic separator...

  5. Synthetic Optimization Model and Algorithm for Railway Freight Center Station Location and Wagon Flow Organization Problem

    Directory of Open Access Journals (Sweden)

    Xing-cai Liu

    2014-01-01

    Full Text Available The railway freight center stations location and wagon flow organization in railway transport are interconnected, and each of them is complicated in a large-scale rail network. In this paper, a two-stage method is proposed to optimize railway freight center stations location and wagon flow organization together. The location model is present with the objective to minimize the operation cost and fixed construction cost. Then, the second model of wagon flow organization is proposed to decide the optimal train service between different freight center stations. The location of the stations is the output of the first model. A heuristic algorithm that combined tabu search (TS with adaptive clonal selection algorithm (ACSA is proposed to solve those two models. The numerical results show the proposed solution method is effective.

  6. A Performance Assessment of an Airborne Separation Assistance System Using Realistic Complex Traffic Flows

    Science.gov (United States)

    Smith, Jeremy C.; Bussink, Frank J. L.

    2008-01-01

    This paper presents the results from a study that investigates the performance of a tactical Airborne Separation Assistance System (ASAS) in en route airspace, under varying demand levels, with realistic traffic flows. The ASAS concept studied here allows flight crews of equipped aircraft to perform separation from other air traffic autonomously. This study addresses the tactical aspects of an ASAS using aircraft state data (i.e. position and velocity) to detect and resolve projected conflicts. In addition, use of a conflict prevention system helps ASAS-equipped aircraft avoid maneuvers that may cause new conflicts. ASAS-capable aircraft are equipped with satellite-based navigation and Automatic Dependent Surveillance Broadcast (ADS-B) for transmission and receipt of aircraft state data. In addition to tactical conflict detection and resolution (CD&R), a complete, integrated ASAS is likely to incorporate a strategic CD&R component with a longer look-ahead time, using trajectory intent information. A system-wide traffic flow management (TFM) component, located at the FAA command center helps aircraft to avoid regions of excessive traffic density and complexity. A Traffic Alert and Collision Avoidance System (TCAS), as used today is the system of last resort. This integrated approach avoids sole reliance on the use of the tactical CD&R studied here, but the tactical component remains a critical element of the complete ASAS. The focus of this paper is to determine to what extent the proposed tactical component of ASAS alone can maintain aircraft separation at demand levels up to three times that of current traffic. The study also investigates the effect of mixing ASAS-equipped aircraft with unequipped aircraft (i.e. current day) that do not have the capability to self-separate. Position and velocity data for unequipped aircraft needs to be available to ASASequipped. Most likely, for this future concept, state data would be available from instrument flight rules (IFR

  7. Method and apparatus for detecting laminar flow separation and reattachment

    Science.gov (United States)

    Stack, John P. (Inventor); Mangalam, Sivaramakrishnan M. (Inventor)

    1990-01-01

    The invention is a method and apparatus for simultaneously detecting laminar separation and reattachment of a fluid stream such as an airstream from and to the upper surface of an airfoil by simultaneously sensing and comparing a plurality of output signals. Each signal represents the dynamic shear stress at one of an equal number of sensors spaced along a straight line on the surface of the airfoil that extends parallel to the airstream. The output signals are simultaneously compared to detect the sensors across which a reversal in phase of said output signal occurs, said detected sensors being in the region of laminar separation or reattachment.

  8. Numerical study and control method of interaction of nucleation and boundary layer separation in condensing flow

    Institute of Scientific and Technical Information of China (English)

    Liansuo AN; Zhi WANG; Zhonghe HAN

    2009-01-01

    The spontaneous nucleation flow in turbine cascade was numerically studied. The model was imple-mented within a full Navier-Stokes viscous flow solution procedure and the process of condensation was calculated by the quadrature method of moments that shows good accuracy with very broad size distributions.Results were presented for viscous and inviscous flow,showing the influence of boundary layer separation and wake vortices on spontaneous nucleation. The results show that the degree of flow separation in wet steam flow is greater than that in superheated steam flow due to condensation shock and that the loss cannot be neglected.Furthermore, the impact of boundary layer separation and wake vortices on velocity profiles and its implications for profile loss were considered. The calculations showed that layer separation and wake vortices influence nucleation rate, leading to different droplet distributions. A method for controlling homogeneous nucleation and for reducing degree of flow separation in high-speed transonic wet steam flow was presented. The liquid phase parameter distribution is sensitive to the suction side profile of turbine cascade, which impacts the nucleation rate distribution leading to different droplet distributions and affects the degree of flow separation. The numerical study provides a practical design method for turbine blade to reduce wetness losses.

  9. Interaction of in-line twin synthetic jets with a separated flow

    Science.gov (United States)

    Wen, Xin; Tang, Hui; Duan, Fei

    2016-04-01

    An experimental investigation is carried out in a water tunnel to investigate the interaction of in-line twin synthetic jets (SJs) with a separated laminar boundary layer over inclined plates. The flow structures induced by the in-line twin SJs at four phase differences and their resulting flow separation delay are examined using dye visualization and particle image velocimetry (PIV) measurements. It is found that, in most of the cases, the heads of hairpin vortices that are produced from the upstream SJ actuator do not change too much, and the vortex legs are highly stretched by the separated shear layer. An exception is the case with 90° phase difference where the combined vortex head appears as a reversed letter "S" and the combined vortex legs are high enough to escape from the influence of the separated flow. Compared to the single SJ, the twin SJs generally exert greater influence on the separated flow regardless of the phase difference. The PIV results in the mid-span plane reveal that the periodic passage of vortex structures influences the separated flow significantly, causing the flapping of the upper edge of the reversed flow region that contributes to the flow separation delay. The delay of separation is also demonstrated by a streak of forward flow protrusion in the wall-parallel PIV results. It is found that the streak varies a lot at different phase differences. Proper orthogonal decomposition analysis is also conducted and two major types of energetic flow structures in the SJ controlled flow are identified: a strip of back-and-forth fluctuation along the upper edge of the reversed flow, and the vortex structures produced by the twin SJs. It is found that the fluctuation strip is most energetic in the single SJ case and the case with 270° phase difference, whereas the convective mode pair is most energetic in the case with 90° phase difference.

  10. The effect of unified power flow controller location in power systems

    Energy Technology Data Exchange (ETDEWEB)

    Tumay, M. [Cukurova University, Baliai-Adana (Turkey). Department of Electrical and Electronic Engineering; Vural, A.M. [Gaziantep University (Turkey). Department of Electrical and Electronic Engineering; Lo, K.L. [University of Strathclyde, Glasgow (United Kingdom). Department of Electrical and Electronic Engineering

    2004-09-01

    The Unified Power Flow Controller (UPFC) is a device that is capable of providing control of voltage magnitude, active and reactive power flows. This paper represents the effect of UPFC location in steady-state analysis and to demonstrate the capabilities of UPFC in controlling active and reactive power flow within any electrical network. In this paper, a complete power injection model of UPFC including both the series injection branch and the shunt exciting branch is derived in rectangular form. An injected power model method is used to represent UPFC in load flow program. Finally, different types of simulation tasks are carried out based on IEEE 30-bus test system. (author)

  11. Quantification of cerebral blood flow by flow-sensitive alternating inversion recovery exempting separate T1 measurement in healthy volunteers

    Institute of Scientific and Technical Information of China (English)

    XIAO Jiang-xi; ZHANG Xue-hui; XIE Sheng; ZOU Run-lei

    2006-01-01

    Background The feasibility of the mapping of quantitative cerebral blood flow (CBF) named flow-sensitive alternating inversion recovery exempting separate T1 measurement (FAIREST) is still controversial. This study aimed to evaluate the reliability of FAIREST in the measurement of regional CBF (rCBF) in healthy volunteers.Methods Eighteen healthy volunteers underwent magnetic resonance (MR) scanning with the sequence of FAIREST. While they were at rest, rCBF values were obtained in various brain regions of interest (ROIs). The same scheme was repeated on every subject after two weeks. Statistical analysis was made to determine the effect of location, scan and side on the measurement of rCBF.Results The mean CBF values were (122 ± 28) ml · (100 g)-1 · min-1 and (43 ±10) ml · (100 g)-1 · min-1 in the gray and white matter respectively. There was significant main effect of location (t=-12.5, P<0.01), but no significant effect of side. Paired t-test of ROIs in the same slice showed no significant difference in most sites between two scans, except in the gray matter of the bilateral frontal lobes (t=2.18-2.34, P <0.05). However, the rCBF values of the same structure obtained from different slices showed a significant difference (t=-3.49,P<0.01).Conclusion FAIREST is a reliable technique in the measurement of rCBF, but different imaging slice may affect the agreement of rCBF across the scans.

  12. The Dynamics of Controlled Flow Separation within a Diverter Duct Diffuser

    Science.gov (United States)

    Peterson, C. J.; Vukasinovic, B.; Glezer, A.

    2016-11-01

    The evolution and receptivity to fluidic actuation of the flow separation within a rectangular, constant-width, diffuser that is branched off of a primary channel is investigated experimentally at speeds up to M = 0.4. The coupling between the diffuser's adverse pressure gradient and the internal separation that constricts nearly half of the flow passage through the duct is controlled using a spanwise array of fluidic actuators on the surface upstream of the diffuser's inlet plane. The dynamics of the separating surface vorticity layer in the absence and presence of actuation are investigated using high-speed particle image velocimetry combined with surface pressure measurements and total pressure distributions at the primary channel's exit plane. It is shown that the actuation significantly alters the incipient dynamics of the separating vorticity layer as the characteristic cross stream scales of the boundary layer upstream of separation and of the ensuing vorticity concentrations within the separated flow increase progressively with actuation level. It is argued that the dissipative (high frequency) actuation alters the balance between large- and small-scale motions near separation by intensifying the large-scale motions and limiting the small-scale dynamics. Controlling separation within the diffuser duct also has a profound effect on the global flow. In the presence of actuation, the mass flow rate in the primary duct increases 10% while the fraction of the diverted mass flow rate in the diffuser increases by more than 45% at 0.7% actuation mass fraction. Supported by the Boeing Company.

  13. Flow-induced phase separation in polymer solutions

    NARCIS (Netherlands)

    Moel, K. de; Flikkema, E.; Szleifer, I.; Brinke, G. ten

    1998-01-01

    A correct description of phase behaviour in polymer solutions requires a coupling between configurational statistics and thermodynamics. The effect of flow-induced chain deformation on the polymer-solvent interaction energy depends on the concentration and on the polymer architecture. It will be dem

  14. Modeling river dune evolution using a parameterization of flow separation

    NARCIS (Netherlands)

    Paarlberg, Andries J.; Dohmen-Janssen, C. Marjolein; Hulscher, Susanne J.M.H.; Termes, Paul

    2009-01-01

    This paper presents an idealized morphodynamic model to predict river dune evolution. The flow field is solved in a vertical plane assuming hydrostatic pressure conditions. The sediment transport is computed using a Meyer-Peter–Müller type of equation, including gravitational bed slope effects and a

  15. Boundary layer separation and reattachment detection on airfoils by thermal flow sensors.

    Science.gov (United States)

    Sturm, Hannes; Dumstorff, Gerrit; Busche, Peter; Westermann, Dieter; Lang, Walter

    2012-10-24

    A sensor concept for detection of boundary layer separation (flow separation, stall) and reattachment on airfoils is introduced in this paper. Boundary layer separation and reattachment are phenomena of fluid mechanics showing characteristics of extinction and even inversion of the flow velocity on an overflowed surface. The flow sensor used in this work is able to measure the flow velocity in terms of direction and quantity at the sensor's position and expected to determine those specific flow conditions. Therefore, an array of thermal flow sensors has been integrated (flush-mounted) on an airfoil and placed in a wind tunnel for measurement. Sensor signals have been recorded at different wind speeds and angles of attack for different positions on the airfoil. The sensors used here are based on the change of temperature distribution on a membrane (calorimetric principle). Thermopiles are used as temperature sensors in this approach offering a baseline free sensor signal, which is favorable for measurements at zero flow. Measurement results show clear separation points (zero flow) and even negative flow values (back flow) for all sensor positions. In addition to standard silicon-based flow sensors, a polymer-based flexible approach has been tested showing similar results.

  16. Topological analysis of plasma flow control on corner separation in a highly loaded compressor cascade

    Institute of Scientific and Technical Information of China (English)

    Xiao-Hu Zhao; Yun Wu; Ying-Hong Li; Xue-De Wang; Qin Zhao

    2012-01-01

    In this paper,flow behavior and topology structure in a highly loaded compressor cascade with and without plasma aerodynamic actuation (PAA) are investigated.Streamline pattern,total pressure loss coefficient,outlet flow angle and topological analysis are considered to study the effect and mechanism of the plasma flow control on corner separation.Results presented include the boundary layer flow behavior,effects of three types of PAA on separated flows and performance parameters,topology structures and sequences of singular points with and without PAA.Two separation lines,reversed flow and backflow exist on the suction surface.The cross flow on the endwall is an important element for the corner separation.PAA can reduce the undertuming and overturning as well as the total pressure loss,leading to an overall increase of flow turning and enhancement of aerodynamic performance.PAA can change the topology structure,sequences of singular points and their corresponding separation lines.Types Ⅱ and Ⅲ PAA are much more efficient in controlling corner separation and enhancing aerodynamic performances than type Ⅰ.

  17. Numerical Simulation and Experimental Investigation of 3-D Separated Flow Field around a Blunt Body

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    @@Motivated by re-designing a fuselage in engineering application, the numerical and experimental investigation of the separated flow field around a special blunt body is described in this thesis. The aerodynamic response of the blunt body is successively studied. The thesis consists of four parts: the numerical simulation of the flow field around a two-dimensional blunt body; the numerical simulation of the flow field around a three-dimensional blunt body; the flow

  18. Experimental evaluation of streamline patterns and separated flows in a series of branching vessels with implications for atherosclerosis and thrombosis.

    Science.gov (United States)

    el-Masry, O A; Feuerstein, I A; Round, G F

    1978-10-01

    Flow conditions in four models representing the aortic bifurcation, iliac bifuraction, and a renal artery branch were investigated at volumetric flow rates corresponding to Reynolds numbers from 1000 to 4000 over the complete range of flow division between daughter vessels. Qualitative flow streamline patterns and quantitative definition of those flow conditions leading to disturbed flow (flow separation ) were determined primarily at steady flow with a limited set of pulsatie experiments. Under conditions of no flow separation, common characteristic streamline patterns not parallel to the center lines of parent or daughter tubes were found for all models. These effects were accentuated with increasing Reynolds number. Flow separation was inducible through alteration of flow division between daughter vessels or by an increase in flow rate. Each of the four models had distinct combinations of flow division ratio and flow rate which gave: (1) no flow separation, (2) flow separation at the outside of the right daughter tube, and (3) flow separation at the outside of the left daughter tube. Models representing the renal artery also had regions of simultaneous left- and righthand separation on the outside of their daughter tubes. The separated flows observed here displayed streamlines forming an open vortex with flows entering and leaving. These regions, which occur only at distinct combinations of flow rate and flow division, may be key centers where platelet aggregates may form, release constituents, and cause vessel injury.

  19. Effect of Riblets upon Flow Separation in a Subsonic Diffuser

    Science.gov (United States)

    1988-12-01

    Technology Air University In Partial Fulfillment of the Requirements for the Degree of Master of Science in Aeronautical Engineering Nathan W. Martens...afterburner where the flow leaving the turbine must be 4 slowed from a high subsonic Mach number to a Mach number of about 0.2" (8:305). Physicall ., a...Riblets," Proceedings of the AIAA 20th Aerospace Sciences Meeting. Paper No. 82-0169. New York: American Institute of Aeronautics and Astronautics

  20. Flow processes in overexpanded chemical rocket nozzles. Part 3: Methods for the aimed flow separation and side load reduction

    Science.gov (United States)

    Schmucker, R. H.

    1983-01-01

    Methods aimed at reduction of overexpansion and side load resulting from asymmetric flow separation for rocket nozzles with a high opening ratio are described. The methods employ additional measures for nozzles with a fixed opening ratio. The flow separation can be controlled by several types of nozzle inserts, the properties of which are discussed. Side loads and overexpansion can be reduced by adapting the shape of the nozzle and taking other additional measures for controlled separation of the boundary layer, such as trip wires.

  1. Staggered car-following induced by lateral separation effects in traffic flow

    Science.gov (United States)

    Jin, Sheng; Wang, Dian-hai; Xu, Cheng; Huang, Zhi-yi

    2012-01-01

    This Letter develops a new staggered car-following model taking into consideration lateral separation effects. Time-to-collision, calculated using visual angle variables, is introduced to describe the lateral separation distance and improve the optimal velocity model. The analytical and numerical results show that the stability of traffic flow can gradually be enhanced with the increase of lateral separation effects. The asymmetry property of traffic flow is also investigated using the new model. The results imply that incorporating lateral separation effects into the car-following model leads to the suppression of traffic jams and greatly enhances the realism of the model.

  2. Microscale phase separator for selective extraction of CO2 from methanol solution flow

    Science.gov (United States)

    Fazeli, Abdolreza; Moghaddam, Saeed

    2014-12-01

    This study is aimed at understanding the limits in reducing the size of a membrane-based CO2 separator and its pressure head needs and energy consumption, while maximizing its selectivity. The separator consists of a flow channel capped by a hydrophobic nanoporous membrane through which CO2 exits the anode flow stream of a direct methanol fuel cell (DMFC). A systematic study is conducted to determine the effect of differential pressure across the membrane, flow velocity, and flow channel dimensions on the separation process. The extraction flux was found to change linearly with pressure difference across the membrane. The effect of flow velocity on the extraction flux was negligible up to a critical velocity beyond which the separation process ceased. The separation selectivity enhanced by increasing the differential pressure across the membrane but did not change with varying the flow velocity and channel depth. Using the findings of the first part of the study, an optimal micro-separator (with a footprint of approximately 10 × 1 mm2) was designed/fabricated for a 20 W DMFC and its performance was experimentally analyzed. An unprecedented separation selectivity of close to 200 was achieved at a differential pressure of about 10 kPa and negligible energy consumption.

  3. Unsteady separated boundary layer in a transonic diffuser flow with self-excited oscillations

    Science.gov (United States)

    Hsieh, T.; Coakley, T. J.

    1986-01-01

    A numerical investigation of two-dimensional unsteady boundary layer in a transonic diffuser flow with self-excited oscillations and strong flow separation by solving the compressible, Reynolds-averaged, thin-layer Navier-Stokes equations with two-equations turbulence model is described. Three different meshes with constant streamwise mesh distribution and varying vertical mesh distribution were used. Results obtained indicate that a refinement of mesh studied here has minimal effect on the mean boundary layer flow but significantly increases the amplitude of oscillation of all flow variables. Comparisons of unsteady wall pressure, velocity profile, terminal shock, and separation pocket among computations and with experiment are presented.

  4. Structure of Three-Dimensional Separated Flow on Symmetric Bumps

    Science.gov (United States)

    2005-11-30

    calculated using N - tan(SSA) -ni(/W/iy) t (1 N- - = - --(4 1 )tan(F(;A) - UII /(LI/’) where vih and 17’- are the stream-wise and span-wise eddy...velocity vectors only in bimodal region and the black lines are connecting these vectors for visual aid . As expected, the two flow patterns are totally... aid only at different yi of. Figure 5.7 Normalized FrF + IWk- vectors locally tangent to surface. 5. LDV Measurements near Large Bump#3 Surface 160

  5. Correlations between wind flow and population location at 67 light water nuclear power plant sites. [USA

    Energy Technology Data Exchange (ETDEWEB)

    Sprung, J.L.; Steck, G.P.; Frazier, A.W.

    1978-10-01

    Because wind flow and population location are both likely to be influenced by topography, it has been suggested that wind distributions and population distributions ought to be correlated and that the neglect of these correlations in the calculations of the Reactor Safety Study could have resulted in significant underestimates of accident consequences. This paper presents the results of an investigation of correlations between wind roses and population locations at 67 of the 68 power plant sites included in the Reactor Safety Study.

  6. Separated flow behind a backward-facing step under a stationary temperature disturbance

    Science.gov (United States)

    Boiko, A. V.; Dovgal, A. V.; Sorokin, A. M.

    2015-11-01

    The flow in the separation region of laminar boundary layer behind a rectangular backward-facing step has been experimentally examined under temperature non-uniformity of the flow. The data were obtained in a subsonic wind tunnel at Reynolds numbers M ≪ 1. The temperature disturbance was generated using a system of Peltier elements provided on the model surface upstream of the separation line. The effect of heating/cooling of the wall on the mean and fluctuating flow components was evaluated using hot-wire measurements. The experimental data were supplemented with calculations of linear-stability characteristics of model velocity profiles in the separated boundary layer. As a result, the response of the separated flow to a stationary thermal perturbation was revealed.

  7. Numerical study on the flow features of U-beam inertial separator

    Institute of Scientific and Technical Information of China (English)

    陈丽华; 樊建人; 岑可法

    2002-01-01

    Detailed parametric study of three-dimensional gas-particle multiphase flow characteristics in Ubeam tube bundle ntertial separators was conducted by numerical simulation.The carrier phase was treated in the Eulerian frame,the particles were tracked in the Lagrangian frame,and particle-wall collision was considered.Simulation carried out at different inflow rate and mass loading ratios revealed the pressure losses in the separators,velocity field of the gas phase,and the trajectories of particles.The study results revealed the multiphase flow-dynamic features of the separators,and the relationship between separator pressure losses and different inlet velocity.The numerical simulation can provde basis both for optimal disign of mpacting-inertial separator used in circulating fluidized bed boiler;and for study of gas-particle multiphase circumfluence flow.

  8. Numerical study on the flow features of U-beam inertial separator

    Institute of Scientific and Technical Information of China (English)

    陈丽华; 樊建人; 岑可法

    2002-01-01

    Detailed parametric study of three-dimensional gas-particle multiph as e flow characteristics in U-beam tube bundle inertial separators was conducted b y numerical simulation. The carrier phase was treated in the Eulerian frame, the particles were tracked in the Lagrangian frame, and particle-wall collision wa s considered. Simulation carried out at different inflow rate and mass loading r atios revealed the pressure losses in the separators, velocity field of the gas phase, and the trajectories of particles. The study results revealed the multiph ase flow-dynamic features of the separators, and the relationship between separ a tor pressure losses and different inlet velocity. The numerical simulation can p rovide basis both for optimal design of impacting-inertial separator used in ci r culating fluidized bed boiler; and for study of gas-particle multiphase circumf luence flow.

  9. Wall-Pressure-Array Measurements Beneath a Separating/Reattaching Flow Region

    Science.gov (United States)

    Hudy, Laura M.; Naguib, Ahmed M.; Humphreys, William M., Jr.; Bartram, Scott M.

    2002-01-01

    A database of wall-pressure array measurements was compiled for studying the space-time character of the surface-pressure field within a separating/reattaching flow region. The experimental setup consisted of a long splitter plate instrumented with an array of 80 flush-mounted microphones located within the wake of a fence. Data were acquired for a Reynolds number of 7885, based on the fence height. Two distinctive regions, defined based on their location relative to the position of the mean reattachment point (xr) of the shear layer, emerged from this investigation. Upstream, from the fence to 1/4xr, the surface-pressure signature was dominated by large time scale disturbances and an upstream convecting velocity of 0.21Uinfinity. Beyond 1/4xr, turbulent structures with small time scales and a downstream convection velocity of 0.57Uinfinity generated most of the pressure fluctuations. There was evidence that these structures began to form around 1/4xr and grew in strength and size with downstream distance before reattaching on the plate. Only the time-averaged results from the microphones have been examined hitherto and will be presented.

  10. Efficiency of energy separation at compressible gas flow in a planar duct

    Science.gov (United States)

    Makarov, M. S.; Makarova, S. N.

    2014-12-01

    The method of energy separation in a high-speed flow proposed by A.I. Leontyev is investigated numerically. The adiabatic compressible gas flow (of a helium-xenon mixture) with a low Prandtl number in a planar narrow duct and a flow with heat exchange in a duct partitioned by a heat-conducting wall are analysed. The temperature recovery factor on the adiabatic wall, degree of cooling the low-speed flow part, temperature efficiency, and the adiabatic efficiency in a duct with heat exchange are estimated. The data are obtained for the first time, which make it possible to compare the efficiency of energy separation in a high-speed flow with the efficiency of similar processes in vortex tubes and other setups of gas-dynamic energy separation.

  11. Gas separation by the molecular exchange flow through micropores of the membrane

    Science.gov (United States)

    Matsumoto, Michiaki; Nakaye, Shoeji; Sugimoto, Hiroshi

    2016-11-01

    A model gas separator that makes use of the molecular exchange flow through porous membrane of 18 cm2 area is fabricated. The gas separator performance is tested for helium-neon mixture. The separator divides a continuous flow of gas mixture into two flows of different gases. The difference of mole percentage is around 8 % at the volumetric feed flow rate of 1 sccm. In the present system, the molecular exchange flow is induced in two Knudsen pumps, where the mixed cellulose ester membrane is used as the thermal transpiration material. The experiment demonstrates the capability of these pumps to increase the concentration of heavy and light molecules, respectively, from the feed mixture.

  12. Development of the Two Phase Flow Separator Experiment for a Reduced Gravity Aircraft Flight

    Science.gov (United States)

    Golliher, Eric; Gotti, Daniel; Owens, Jay; Gilkey, Kelly; Pham, Nang; Stehno, Philip

    2016-01-01

    The recent hardware development and testing of a reduced gravity aircraft flight experiment has provided valuable insights for the future design of the Two Phase Flow Separator Experiment (TPFSE). The TPFSE is scheduled to fly within the Fluids Integration Rack (FIR) aboard the International Space Station (ISS) in 2020. The TPFSE studies the operational limits of gas and liquid separation of passive cyclonic separators. A passive cyclonic separator utilizes only the inertia of the incoming flow to accomplish the liquid-gas separation. Efficient phase separation is critical for environmental control and life support systems, such as recovery of clean water from bioreactors, for long duration human spaceflight missions. The final low gravity aircraft flight took place in December 2015 aboard NASA's C9 airplane.

  13. Numerical Study on Particle Motions in Swirling Flows in a Cyclone Separator

    Institute of Scientific and Technical Information of China (English)

    Kazuyoshi MATSUZAKI; Hideaki USHIJIMA; Mizue MUNEKATA; Hideki OHBA

    2006-01-01

    The purpose of this study is to establish the high-accurate prediction method of particle separation in a cyclone separator. Numerical simulation of the swirling flows in a cyclone separator is performed by using a large eddy simulation (LES) based on a Smagorinsky model. The validity of the simulation and the complicated flow characteristics are discussed by comparison with experimental results. Moreover, particle motions are treated by a Lagrangian method and are calculated with a one-way method. A performance for particle separation is predicted from the results of the particle tracing. As results of our investigation, the influences of the inserted height of the outlet pipe on the performance for particle separation of cyclone separator are shown.

  14. Influence of nose-perturbation location on behavior of vortical flow around slender body at high incidence

    Institute of Scientific and Technical Information of China (English)

    GUAN XiaoRong; XU Cheng; WANG YongJuan; WANG YaPing

    2009-01-01

    Response of the vortical flow around a slender body of revolution at high incidence to the shift of a single nose perturbation was investigated systematically using numerical methods. A minute geometric bump was employed to act as the nose perturbation, and all computations were performed for subsonic flows at incidence of 50°. The computational results show that the vortical flow is more sensitive to the perturbation located axially closer to the nose apex of a slender body. With perturbation shifting axially downstream away from nose apex, there is a critical axial location appearing. The vortical flow is less sensitive to the perturbation located axially closer to the critical axial location; when perturbation traverses axially around the critical axial location, the vortical flow switches between opposite asymmetric patterns. The eventual influence of perturbation axial location on the vortical flow lies on both its relative locations to nose apex and the critical axial location. The vortical flow is more sensitive to the perturbation located circumferentially farther away from the fore-and-aft symmetric plane of a slender body, and just the asymmetrically-located perturbation can provoke the vortical flow to asymmetry. With perturbation shifting circumferentially in sequence, the vortical flow varies by degrees in manner of a single periodicity. A convective-type of instability existing in the flow field is responsible for the influence of nose perturbation on the vortical flow.

  15. Vortex-breakdown and wall-separation states in swirling flows in a straight pipe

    Science.gov (United States)

    Zhang, Yuxin; Rusak, Zvi; Wang, Shixiao

    2016-11-01

    The appearance of vortex-breakdown and wall-separation states in various incoming swirling flows to a straight circular pipe is investigated. Fixed-in-time profiles of the axial and circumferential velocities and of the azimuthal vorticity are prescribed at the pipe inlet. A parallel flow state is set at the pipe outlet. Following the theory of Wang & Rusak (1997), the outlet state of the steady flow problem is determined for a long pipe by solutions of the columnar (axially-independent) Squire-Long equation. For each of the incoming flows studied, these solutions include the base columnar flow state, a decelerated flow along the centerline, an accelerated flow along the centerline, a vortex-breakdown state and a wall-separation state. These theoretical predictions are numerically realized by flow simulations based on the unsteady flow equations. The simulations shed light on the base flow stability and the dynamics of initial perturbations to the various states. The present study extends all the six bifurcation diagrams of solutions studied in Leclaire & Sipp (2010), who stopped the development of branches of steady states once breakdown and wall-separation states first appear.

  16. Impact of trucking network flow on preferred biorefinery locations in the southern United States

    Science.gov (United States)

    Timothy M. Young; Lee D. Han; James H. Perdue; Stephanie R. Hargrove; Frank M. Guess; Xia Huang; Chung-Hao Chen

    2017-01-01

    The impact of the trucking transportation network flow was modeled for the southern United States. The study addresses a gap in existing research by applying a Bayesian logistic regression and Geographic Information System (GIS) geospatial analysis to predict biorefinery site locations. A one-way trucking cost assuming a 128.8 km (80-mile) haul distance was estimated...

  17. Separation of base flow from streamflow using groundwater levels - illustrated for the Pang catchment (UK)

    NARCIS (Netherlands)

    Peters, E.; Lanen, van H.A.J.

    2005-01-01

    A new filter to separate base flow from streamflow has developed that uses observed groundwater levels. To relate the base flow to the observed groundwater levels, a non-linear relation was used. This relation is suitable for unconfined aquifers with deep groundwater levels that do not respond to

  18. Separation of base flow from streamflow using groundwater levels - illustrated for the Pang catchment (UK)

    NARCIS (Netherlands)

    Peters, E.; Lanen, van H.A.J.

    2005-01-01

    A new filter to separate base flow from streamflow has developed that uses observed groundwater levels. To relate the base flow to the observed groundwater levels, a non-linear relation was used. This relation is suitable for unconfined aquifers with deep groundwater levels that do not respond to in

  19. Large Eddy Simulation of the vortex end in reverse-flow centrifugal separators

    NARCIS (Netherlands)

    Pisarev, A.V.; Hoffmann, A.C.; Peng, W.; Dijkstra, H.A.|info:eu-repo/dai/nl/073504467

    2011-01-01

    The present work is a study of the gas-flow phenomenon known as the “end of the vortex” (EoV), which spontaneously occurs at the lower end, or under, reverse-flow centrifugal separators such as cyclones or swirl tubes. Different CFD models of swirl tubes have been built to study and analyse this

  20. Numerical Study of Control of Flow Separation Over a Ramp with Nanosecond Plasma Actuator

    Science.gov (United States)

    Zheng, J. G.; Khoo, B. C.; Cui, Y. D.; Zhao, Z. J.; Li, J.

    2016-06-01

    The nanosecond plasma discharge actuator driven by high voltage pulse with typical rise and decay time of several to tens of nanoseconds is emerging as a promising active flow control means in recent years and is being studied intensively. The characterization study reveals that the discharge induced shock wave propagates through ambient air and introduces highly transient perturbation to the flow. On the other hand, the residual heat remaining in the discharge volume may trigger the instability of external flow. In this study, this type of actuator is used to suppress flow separation over a ramp model. Numerical simulation is carried out to investigate the interaction of the discharge induced disturbance with the external flow. It is found that the flow separation region over the ramp can be reduced significantly. Our work may provide some insights into the understanding of the control mechanism of nanosecond pulse actuator.

  1. Optimising location of unified power flow controllers by means of improved evolutionary programming

    Energy Technology Data Exchange (ETDEWEB)

    Hao, J.; Chen, C. [Shanghai Jiaotong Univ. (China). Dept. of Electrical Engineering; Shi, L.B. [Hong Kong Univ. (China). Dept. of Electrical and Electronic Engineering

    2004-11-01

    The unified power flow controller (UPFC) is one of the most promising Flexible AC Transmission Systems (FACTS) devices for the load flow control. Simultaneous optimisation of location and parameters for UPFCs is an important issue when the given number of UPFCs is applied to the power system with the purpose of increasing system loadability. This paper presents a mathematical model about optimal location and parameters of UPFCs to maximise the system loadability subject to the transmission line capacity limits and specified voltage level. An improved computational intelligence approach: self-adaptive evolutionary programming (SAEP) is used to solve the nonlinear programming problem presented above for better accuracy. Case studies of the IEEE 30- and 118-bus systems using the proposed model and technique demonstrate that the proposed mathematical model is corrective and efficient. Furthermore, steady-state performance of power system can be effectively enhanced due to the optimal location and parameters of UPFCs. (author)

  2. Gas-liquid two-phase flows in double inlet cyclones for natural gas separation

    DEFF Research Database (Denmark)

    Yang, Yan; Wang, Shuli; Wen, Chuang

    2017-01-01

    The gas-liquid two-phase flow within a double inlet cyclone for natural gasseparation was numerically simulated using the discrete phase model. The numericalapproach was validated with the experimental data, and the comparison resultsagreed well with each other. The simulation results showed......-outlet. The swirling flow was concentric dueto the design of the double inlet for the cyclonic separator, which greatly improvedthe separating efficiency. The separating efficiency was greater than 90% with theparticle diameter of more than 100 μm....

  3. Effect of swirling device on flow behavior in a supersonic separator for natural gas dehydration

    DEFF Research Database (Denmark)

    Wen, Chuang; Li, Anqi; Walther, Jens Honore;

    2016-01-01

    The supersonic separator is a revolutionary device to remove the condensable components from gas mixtures. One of the key issues for this novel technology is the complex supersonic swirling flow that is not well understood. A swirling device composed of an ellipsoid and several helical blades is ...... the separation performance. When the swirling flow passes through the annular nozzle, it will damage the expansion characteristics of the annular nozzle. The blade angles and numbers are both optimized by evaluating the swirling and expansion effects for the supersonic separation....

  4. The influence of bus stop on traffic flow with velocity-difference-separation model

    Science.gov (United States)

    Zheng, Pengjun; Wang, Wei; Ge, Hongxia

    2016-06-01

    Based on velocity-difference-separation model, the mixed traffic flow on two-lane road is investigated. For a fixed road length, the influence of bus and bus stops on traffic flow is studied with the increasing traffic density. Compared with the result without bus stops given by Li et al., a new traffic state is found, which is valuable for studying the impacts of public transport on urban traffic flow.

  5. Reciprocating free-flow isoelectric focusing device for preparative separation of proteins.

    Science.gov (United States)

    Kong, Fan-Zhi; Yang, Ying; Wang, Yi; Li, Guo-Qing; Li, Shan; Xiao, Hua; Fan, Liu-Yin; Liu, Shao-Rong; Cao, Cheng-Xi

    2015-11-27

    The traditional recycling free-flow isoelectric focusing (RFFIEF) suffered from complex structure, tedious operations and poor extensibility as well as high cost. To address these issues, a novel reciprocating free-flow isoelectric focusing device (ReFFIEF) was developed for proteins or peptides pre-fractionation. In the new device, a reciprocating background flow was for the first time introduced into free flow electrophoresis (FFE) system. The gas cushion injector (GCI) used in the previous continuous free-flow electrophoresis (CFFE) was redesigned for the reciprocating background flow. With the GCI, the reciprocating background flow could be achieved between the GCI, separation chamber and transient self-balance collector (tSBC). In a run, process fluid flowed to and from, forming a stable reciprocating fluid flow in the separation chamber. A pH gradient was created within the separation chamber, and at the same time proteins were focused repeatedly when passing through the chamber under perpendicular electric field. The ReFFIEF procedure was optimized for fractionations of three model proteins, and the optimized method was further used for pre-fractionation of model human serum samples. As compared with the traditional RFFIEF devices developed about 25 years ago, the new ReFFIEF system showed several merits, such as simple design and structure, user-friendly operation and easy to extend as well as low cost.

  6. Spontaneous reconnection at a separator current layer. II. Nature of the waves and flows

    CERN Document Server

    Stevenson, Julie E H

    2015-01-01

    Sudden destabilisations of the magnetic field, such as those caused by spontaneous reconnection, will produce waves and/or flows. Here, we investigate the nature of the plasma motions resulting from spontaneous reconnection at a 3D separator. In order to clearly see the perturbations generated by the reconnection, we start from a magnetohydrostatic equilibrium containing two oppositely-signed null points joined by a generic separator along which lies a twisted current layer. The nature of the magnetic reconnection initiated in this equilibrium as a result of an anomalous resistivity is discussed in detail in \\cite{Stevenson15_jgra}. The resulting sudden loss of force balance inevitably generates waves that propagate away from the diffusion region carrying the dissipated current. In their wake a twisting stagnation-flow, in planes perpendicular to the separator, feeds flux back into the original diffusion site (the separator) in order to try to regain equilibrium. This flow drives a phase of slow weak impulsiv...

  7. Membraneless laminar flow cell for electrocatalytic CO2 reduction with liquid product separation

    Science.gov (United States)

    Monroe, Morgan M.; Lobaccaro, Peter; Lum, Yanwei; Ager, Joel W.

    2017-04-01

    The production of liquid fuel products via electrochemical reduction of CO2 is a potential path to produce sustainable fuels. However, to be practical, a separation strategy is required to isolate the fuel-containing electrolyte produced at the cathode from the anode and also prevent the oxidation products (i.e. O2) from reaching the cathode. Ion-conducting membranes have been applied in CO2 reduction reactors to achieve this separation, but they represent an efficiency loss and can be permeable to some product species. An alternative membraneless approach is developed here to maintain product separation through the use of a laminar flow cell. Computational modelling shows that near-unity separation efficiencies are possible at current densities achievable now with metal cathodes via optimization of the spacing between the electrodes and the electrolyte flow rate. Laminar flow reactor prototypes were fabricated with a range of channel widths by 3D printing. CO2 reduction to formic acid on Sn electrodes was used as the liquid product forming reaction, and the separation efficiency for the dissolved product was evaluated with high performance liquid chromatography. Trends in product separation efficiency with channel width and flow rate were in qualitative agreement with the model, but the separation efficiency was lower, with a maximum value of 90% achieved.

  8. Turbine endwall two-cylinder program. [wind tunnel and water tunnel investigation of three dimensional separation of fluid flow

    Science.gov (United States)

    Langston, L. S.

    1980-01-01

    Progress is reported in an effort to study the three dimensional separation of fluid flow around two isolated cylinders mounted on an endwall. The design and performance of a hydrogen bubble generator for water tunnel tests to determine bulk flow properties and to measure main stream velocity and boundary layer thickness are described. Although the water tunnel tests are behind schedule because of inlet distortion problems, tests are far enough along to indicate cylinder spacing, wall effects and low Reynolds number behavior, all of which impacted wind tunnel model design. The construction, assembly, and operation of the wind tunnel and the check out of its characteristics are described. An off-body potential flow program was adapted to calculate normal streams streamwise pressure gradients at the saddle point locations.

  9. Convection Heat Transfer in Three-Dimensional Turbulent Separated/Reattached Flow

    Energy Technology Data Exchange (ETDEWEB)

    Bassem F. Armaly

    2007-10-31

    The measurements and the simulation of convective heat transfer in separated flow have been a challenge to researchers for many years. Measurements have been limited to two-dimensional flow and simulations failed to predict accurately turbulent heat transfer in the separated and reattached flow region (prediction are higher than measurements by more than 50%). A coordinated experimental and numerical effort has been initiated under this grant for examining the momentum and thermal transport in three-dimensional separated and reattached flow in an effort to provide new measurements that can be used for benchmarking and for improving the simulation capabilities of 3-D convection in separated/reattached flow regime. High-resolution and non-invasive measurements techniques are developed and employed in this study to quantify the magnitude and the behavior of the three velocity components and the resulting convective heat transfer. In addition, simulation capabilities are developed and employed for improving the simulation of 3-D convective separated/reattached flow. Such basic measurements and simulation capabilities are needed for improving the design and performance evaluation of complex (3-D) heat exchanging equipment. Three-dimensional (3-D) convective air flow adjacent to backward-facing step in rectangular channel is selected for the experimental component of this study. This geometry is simple but it exhibits all the complexities that appear in any other separated/reattached flow, thus making the results generated in this study applicable to any other separated and reattached flow. Boundary conditions, inflow, outflow, and wall thermal treatment in this geometry can be well measured and controlled. The geometry can be constructed with optical access for non-intrusive measurements of the flow and thermal fields. A three-component laser Doppler velocimeter (LDV) is employed to measure simultaneously the three-velocity components and their turbulent fluctuations

  10. Modification of flow perturbations in a laminar separation bubble by heat transfer

    Science.gov (United States)

    Boiko, A. V.; Dovgal, A. V.; Sorokin, A. M.

    2017-02-01

    Laminar boundary layer separation in conditions of localized heat transfer is investigated at low subsonic velocity through wind-tunnel measurements and linear stability analysis. A backward-facing step flow is subjected to a stationary temperature variation generated by Peltier elements installed on the test model directly downstream of the separation line. The experimental and theoretical data clarify the response of velocity disturbances in the separation region to the temperature variation, the latter appearing primarily as a modifier of the initial wave spectrum of the amplifying separated layer oscillations.

  11. Lab on a chip for continuous-flow magnetic cell separation.

    Science.gov (United States)

    Hejazian, Majid; Li, Weihua; Nguyen, Nam-Trung

    2015-02-21

    Separation of cells is a key application area of lab-on-a-chip (LOC) devices. Among the various methods, magnetic separation of cells utilizing microfluidic devices offers the merits of biocompatibility, efficiency, and simplicity. This review discusses the fundamental physics involved in using magnetic force to separate particles, and identifies the optimisation parameters and corresponding methods for increasing the magnetic force. The paper then elaborates the design considerations of LOC devices for continuous-flow magnetic cell separation. Examples from the recently published literature illustrate these state-of-the-art techniques.

  12. Review of zero-net-mass-flux jet and its application in separation flow control

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Since the zero-net-mass-flux (ZNMF) jet was first used as a laboratory flow control method in 1990’s, it has attracted much attention. The ZNMF jet has unique features such as compact actuator, no requirement for external air supply, complex piping, etc., and becomes a hot topic research subject in fluid mechanics. This review introduces the state of the art in the development of ZNMF jet in the quiescent fluid, the interaction of the ZNMF jet with the cross flow and its application in the separation flow control. The evolution of the vortex ring/pair and the spacial flow structure of the ZNMF in quicent fluid or cross flow are presented, as well as the key parameter effects. At last, the applications of ZNMF jet in the wake control of the circular cylinder, the separation control on the airfoil and the aerodynamic force or moment control on MAV/UAV are presented.

  13. Review of zero-net-mass-flux jet and its application in separation flow control

    Institute of Scientific and Technical Information of China (English)

    ZHANG PanFeng; WANG JinJun; FENG LiHao

    2008-01-01

    Since the zero-net-mass-flux (ZNMF) jet was first used as a laboratory flow control method in 1990's, it has attracted much attention. The ZNMF jet has unique features such as compact actuator, no requirement for external air supply, complex piping, etc., and becomes a hot topic research subject in fluid mechanics. This review introduces the state of the art in the development of ZNMF jet in the quiescent fluid, the interaction of the ZNMF jet with the cross flow and its application in the separation flow control. The evolution of the vortex ring/pair and the spacial flow structure of the ZNMF in quicent fluid or cross flow are presented, as well as the key parameter effects. At last, the applications of ZNMF jet in the wake control of the circular cylinder, the separation control on the airfoil and the aerodynamic force or moment control on MAV/UAV are presented.

  14. Flow separation control by using bowed blade in highly loaded turbine cascades

    Institute of Scientific and Technical Information of China (English)

    YAMAMOTO; Atsumasa

    2009-01-01

    Due to the serious flow separations and centralized vortices,there are high secondary losses in highly loaded turbines.It is imperative to find measures to control the flow separation and vortices hence improve the turbine performance.This paper reports our recent progress on flow separation and vor-tices control in highly loaded turbine cascades by using bowed blades.Two sets of highly loaded tur-bine cascades with the turning angles of 113° and 160°,and each with 7 bowed blade angles 0°(straight),±10°,±20° and ±30° were experimentally investigated.Both internal flow field measurement and flow visualization on the blade surfaces were conducted,and the effects of blade bowing on the flow topology,distribution of vorticity and the flow energy loss were discussed.The results show that,for the cascade with the turning angle of 113°,the appropriately positive bow angle could reduce the flow energy loss;whereas for the cascade with the turning angle of 160°,the well selected negative bow angle can give the better aerodynamic performance.

  15. Flow separation control by using bowed blade in highly loaded turbine cascades

    Institute of Scientific and Technical Information of China (English)

    TAN ChunQing; ZHANG HuaLiang; CHEN HaiSheng; DONG XueZhi; ZHAO HongLei; YAMAMOTO Atsumasa

    2009-01-01

    Due to the serious flow separations and centralized vortices, there are high secondary losses in highly loaded turbines. It is imperative to find measures to control the flow separation and vortices hence improve the turbine performance. This paper reports our recent progress on flow separation and vor-tices control in highly loaded turbine cascades by using bowed blades. Two sets of highly loaded tur-bine cascades with the turning angles of 113°and 160°, and each with 7 bowed blade angles 0°(straight),±10°, ±20° and ±30° were experimentally investigated. Both internal flow field measurement and flow visualization on the blade surfaces were conducted, and the effects of blade bowing on the flow topology, distribution of vorticity and the flow energy loss were discussed. The results show that, for the cascade with the turning angle of 113°, the appropriately positive bow angle could reduce the flow energy loss; whereas for the cascade with the turning angle of 160°, the well selected negative bow angle can give the better aerodynamic performance.

  16. Hypersonic Separated Flows About "Tick" Configurations With Sensitivity to Model Design

    Science.gov (United States)

    Moss, J. N.; O'Byrne, S.; Gai, S. L.

    2014-01-01

    This paper presents computational results obtained by applying the direct simulation Monte Carlo (DSMC) method for hypersonic nonequilibrium flow about "tick-shaped" model configurations. These test models produces a complex flow where the nonequilibrium and rarefied aspects of the flow are initially enhanced as the flow passes over an expansion surface, and then the flow encounters a compression surface that can induce flow separation. The resulting flow is such that meaningful numerical simulations must have the capability to account for a significant range of rarefaction effects; hence the application of the DSMC method in the current study as the flow spans several flow regimes, including transitional, slip, and continuum. The current focus is to examine the sensitivity of both the model surface response (heating, friction and pressure) and flowfield structure to assumptions regarding surface boundary conditions and more extensively the impact of model design as influenced by leading edge configuration as well as the geometrical features of the expansion and compression surfaces. Numerical results indicate a strong sensitivity to both the extent of the leading edge sharpness and the magnitude of the leading edge bevel angle. Also, the length of the expansion surface for a fixed compression surface has a significant impact on the extent of separated flow.

  17. Effect of inlet and outlet flow conditions on natural gas parameters in supersonic separation process.

    Directory of Open Access Journals (Sweden)

    Yan Yang

    Full Text Available A supersonic separator has been introduced to remove water vapour from natural gas. The mechanisms of the upstream and downstream influences are not well understood for various flow conditions from the wellhead and the back pipelines. We used a computational model to investigate the effect of the inlet and outlet flow conditions on the supersonic separation process. We found that the shock wave was sensitive to the inlet or back pressure compared to the inlet temperature. The shock position shifted forward with a higher inlet or back pressure. It indicated that an increasing inlet pressure declined the pressure recovery capacity. Furthermore, the shock wave moved out of the diffuser when the ratio of the back pressure to the inlet one was greater than 0.75, in which the state of the low pressure and temperature was destroyed, resulting in the re-evaporation of the condensed liquids. Natural gas would be the subsonic flows in the whole supersonic separator, if the mass flow rate was less than the design value, and it could not reach the low pressure and temperature for the condensation and separation of the water vapor. These results suggested a guidance mechanism for natural gas supersonic separation in various flow conditions.

  18. Experimental investigations on separation control and flow structure around a circular cylinder with synthetic jet

    Institute of Scientific and Technical Information of China (English)

    WANG; JinJun; FENG; LiHao; XU; ChaoJun

    2007-01-01

    Circular cylinder separation control and flow structure influenced by the synthetic jet have been experimentally investigated in a water channel. The synthetic jet issues from a slot and ejects toward upstream from the front stagnation point of the cylinder. It has been found that, similar to the traditional synthetic jet which is positioned near the separation point or inside the separation region, the present synthetic jet arrangement constitutes an efficient way to control flow separation of the circular cylinder, but with a different control mechanism. The present synthetic jet leads to an upstream displacement of the front stagnation point and the formation of a vortex pair near both sides of the exit orifice. When ReU based on the synthetic jet average exit orifice velocity is about lower than 43, a closed envelope forms in front of the windward side of the cylinder during the blowing cycle of synthetic jet, which acts as an apparent modification for the cylinder configuration. When ReU is high enough, an open envelope forms upstream of the cylinder, and the flow around the cylinder becomes much energetic. Thus, regardless of ReU, the present synthetic jet can improve separation for flow around a circular cylinder. With regard to the leeward side, as ReU increases, the flow separation region behind the cylinder gradually disappears. The flow over cylinder may be fully attached when the open envelope forms upstream of the cylinder and ReU is greater than 344. Then, the flow past the cylinder will converge near the back stagnation point of the cylinder, where a new vortex pair shedding periodically is generated due to the high shear layer.

  19. Unlocking the potential of supported liquid phase catalysts with supercritical fluids: low temperature continuous flow catalysis with integrated product separation.

    Science.gov (United States)

    Franciò, Giancarlo; Hintermair, Ulrich; Leitner, Walter

    2015-12-28

    Solution-phase catalysis using molecular transition metal complexes is an extremely powerful tool for chemical synthesis and a key technology for sustainable manufacturing. However, as the reaction complexity and thermal sensitivity of the catalytic system increase, engineering challenges associated with product separation and catalyst recovery can override the value of the product. This persistent downstream issue often renders industrial exploitation of homogeneous catalysis uneconomical despite impressive batch performance of the catalyst. In this regard, continuous-flow systems that allow steady-state homogeneous turnover in a stationary liquid phase while at the same time effecting integrated product separation at mild process temperatures represent a particularly attractive scenario. While continuous-flow processing is a standard procedure for large volume manufacturing, capitalizing on its potential in the realm of the molecular complexity of organic synthesis is still an emerging area that requires innovative solutions. Here we highlight some recent developments which have succeeded in realizing such systems by the combination of near- and supercritical fluids with homogeneous catalysts in supported liquid phases. The cases discussed exemplify how all three levels of continuous-flow homogeneous catalysis (catalyst system, separation strategy, process scheme) must be matched to locate viable process conditions.

  20. Testing seismic amplitude source location for fast debris-flow detection at Illgraben, Switzerland

    Science.gov (United States)

    Walter, Fabian; Burtin, Arnaud; McArdell, Brian W.; Hovius, Niels; Weder, Bianca; Turowski, Jens M.

    2017-06-01

    Heavy precipitation can mobilize tens to hundreds of thousands of cubic meters of sediment in steep Alpine torrents in a short time. The resulting debris flows (mixtures of water, sediment and boulders) move downstream with velocities of several meters per second and have a high destruction potential. Warning protocols for affected communities rely on raising awareness about the debris-flow threat, precipitation monitoring and rapid detection methods. The latter, in particular, is a challenge because debris-flow-prone torrents have their catchments in steep and inaccessible terrain, where instrumentation is difficult to install and maintain. Here we test amplitude source location (ASL) as a processing scheme for seismic network data for early warning purposes. We use debris-flow and noise seismograms from the Illgraben catchment, Switzerland, a torrent system which produces several debris-flow events per year. Automatic in situ detection is currently based on geophones mounted on concrete check dams and radar stage sensors suspended above the channel. The ASL approach has the advantage that it uses seismometers, which can be installed at more accessible locations where a stable connection to mobile phone networks is available for data communication. Our ASL processing uses time-averaged ground vibration amplitudes to estimate the location of the debris-flow front. Applied to continuous data streams, inversion of the seismic amplitude decay throughout the network is robust and efficient, requires no manual identification of seismic phase arrivals and eliminates the need for a local seismic velocity model. We apply the ASL technique to a small debris-flow event on 19 July 2011, which was captured with a temporary seismic monitoring network. The processing rapidly detects the debris-flow event half an hour before arrival at the outlet of the torrent and several minutes before detection by the in situ alarm system. An analysis of continuous seismic records furthermore

  1. Separation of cancer cells from white blood cells by pinched flow fractionation

    DEFF Research Database (Denmark)

    Jensen, Marie Pødenphant; Ashley, Neil; Koprowska, Kamila

    2015-01-01

    In this paper, the microfluidic size-separation technique pinched flow fractionation (PFF) is used to separate cancer cells from white blood cells (WBCs). The cells are separated at efficiencies above 90% for both cell types. Circulating tumor cells (CTCs) are found in the blood of cancer patients...... is challenged by the size overlap between cancer cells and the 106 times more abundant WBCs. The size overlap prevents high efficiency separation, however we demonstrate that cell deformability can be exploited in PFF devices to gain higher efficiencies than expected from the size distribution of the cells....... and can form new tumors. CTCs are rare cells in blood, but they are important for the understanding of metastasis. There is therefore a high interest in developing a method for the enrichment of CTCs from blood samples, which also enables further analysis of the separated cells. The separation...

  2. A potential-flow/boundary-layer method for calculating subsonic and transonic airfoil flow with trailing-edge separation

    Science.gov (United States)

    Barnwell, R. W.

    1981-01-01

    The development of a potential-flow/boundary-layer method for calculating subsonic and transonic turbulent flow past airfoils with trailing-edge separation is reported. A moment-of-momentum integral boundary-layer method is used which employs the law-of-the-wall/law-of-the-wake velocity profile and a two-layer eddy-viscosity model and ignores the laminar sublayer. All integrals across the boundary layer are obtained in closed form. Separation is assumed to occur when the shearing-stress velocity vanishes. A closed-form solution is derived for separated-flow regions where the shearing stress is negligible. In the potential-flow method, the exact form of the airfoil boundary condition is used, but it is applied at the chord line rather than the airfoil surface. This allows the accurate computation of flow about airfoils at large angles of attack but permits the use of body-oriented Cartesian computational grids. The governing equation for the perturbation velocity potential contains several terms in addition to the classical small-disturbance terms.

  3. On the use of wall functions as boundary conditions for two-dimensional separated compressible flows

    Science.gov (United States)

    Viegas, J. R.; Rubesin, M. W.; Horstman, C. C.

    1985-01-01

    A new and improved wall function method for compressible turbulent flows has been developed and tested. This method is applicable to attached and separated flows, to both high- and low-Reynolds number flows, and to flows with adiabatic and nonadiabatic surfaces. This wall function method has been applied to the Launder-Spalding k-epsilon two-equation model of turbulence. The tests consist of comparisons of calculated and experimental results for: (1) an axisymmetrical transonic shock-wave/boundary-wave interaction flow at low Reynolds number in an adiabatic tube, (2) an axisymmetrical high-Reynolds number transonic flow over a nonadiabatic bump, and (3) a two-dimensional supersonic high-Reynolds number flow on a nonadiabatic deflected flap. Each of these experiments had significant regions of flow separation. The calculations are performed with an implicit algorithm that solves the Reynolds-averaged Navier-Stokes equations. It is shown that the results obtained agree very well with the data for the complex compressible flows tested.

  4. A new secure access method in locator/ID separation network%分离机制下一种互联网安全接入方法

    Institute of Scientific and Technical Information of China (English)

    王凯; 高阳阳; 秦雅娟

    2011-01-01

    In the Locator/ID Separation Network, the authors design a new structure for Access Identifier, .which is unique and unchangeable, and propose a new secure access method. The related protocol flow and protocol format are presented in the paper. Finally, the authors study the security performance of the proposed methodusing SVO logic, and show that it can guarantee authenticity of IP packet's source address in Locator/ID Separation Network.%重点研究身份与位置分离机制下源地址真实性保障方面的方法,提出了身份与位置分离网络中唯一且不变的终端身份标识EID结构,并设计了一种保障源地址真实性的安全接入方法,并且给出了相应的协议流程和协议格式,保证了身份与位置分离网络中源地址即终端身份标识EID的真实性.最后使用SVO形式化逻辑对其安全性进行了证明.

  5. Comparison of Miniaturized and Conventional Asymmetrical Flow Field-Flow Fractionation (AF4 Channels for Nanoparticle Separations

    Directory of Open Access Journals (Sweden)

    Zengchao You

    2017-03-01

    Full Text Available The performance of a miniaturized channel for the separation of polymer and metal nanoparticles (NP using Asymmetrical Flow Field-Flow Fractionation (AF4 was investigated and compared with a conventional AF4 system. To develop standard separation methods, experimental parameters like cross flow, gradient profile and injection time were varied and optimized. Corresponding chromatographic parameters were calculated and compared. Our results indicate that the chromatographic resolution in the miniaturized channel is lower, whereas significantly shorter analyses time and less solvent consumption were obtained. Moreover, the limit of detection (LOD and limit of quantification (LOQ obtained from hyphenation with a UV-detector are obviously lower than in a conventional channel, which makes the miniaturized channel interesting for trace analysis.

  6. Application of DDES and IDDES with shear layer adapted subgrid length-scale to separated flows

    Science.gov (United States)

    Guseva, E. K.; Garbaruk, A. V.; Strelets, M. Kh

    2016-11-01

    A comparative study is conducted of the original versions of Delayed Detached- Eddy Simulation (DDES) and Improved DDES (IDDES) and these approaches combined with “shear-layer-adapted” (SLA) subgrid length-scale proposed recently for resolving the issue of delayed RANS-to-LES transition in separated shear layers in global hybrid RANS-LES approaches. Computations were carried out of two separated flows: a transonic flow past M 219 cavity and a subsonic flow over NASA wall mounted hump. Results of the computations suggest that the use of the SLA subgrid length-scale considerably accelerates transition to resolved three-dimensional turbulence in the separated shear layers and substantially improves agreement with the experimental data.

  7. Optimization of hydrogen stations in Florida using the Flow-Refueling Location Model

    Energy Technology Data Exchange (ETDEWEB)

    Kuby, Michael; Kim, Jong-Geun [School of Geographical Sciences, Arizona State University, Tempe, AZ 85287-5302 (United States); Lines, Lee [Department of Environmental Studies, Rollins College, 1000 Holt Ave., Box 2753, Winter Park, FL 32789-4499 (United States); Schultz, Ronald; Xie, Zhixiao [Department of Geosciences, Florida Atlantic University, Boca Raton, FL 33431 (United States); Lim, Seow [Salt River Project, 1521 N. Project Drive, Tempe, AZ 85281-1298 (United States)

    2009-08-15

    This paper develops and applies a model that locates hydrogen stations to refuel the maximum volume of vehicle flows. Inputs to the model include a road network with average speeds; the origin-destination flow volumes between each origin and destination; a maximum driving range between refueling stops; and the number of stations to build. The Flow-Refueling Location Model maximizes the flow volumes that can be refueled, measured either in number of trips or vehicle-miles traveled. Geographic Information Systems and heuristic algorithms are integrated in a spatial decision support system that researchers can use to develop data, enter assumptions, analyze scenarios, evaluate tradeoffs, and map results. For the Florida Hydrogen Initiative, we used this model to investigate strategies for rolling out an initial refueling infrastructure in Florida at two different scales of analysis: metropolitan Orlando and statewide. By analyzing a variety of scenarios at both scales of analysis, we identify a robust set of stations that perform well under a variety of assumptions, and develop a strategy for phasing in clustered and connecting stations in several stages or tiers. (author)

  8. SIMULATION OF OIL-WATER TWO PHASE FLOW AND SEPARATION BEHAVIORS IN COMBINED T JUNCTIONS

    Institute of Scientific and Technical Information of China (English)

    CHEN Jian-lei; HE Li-min; LUO Xiao-ming; BAI Hai-tao; WEI Yan-hai

    2012-01-01

    The combined T junctions used for the oil-water separation have the advantages of compactness in structure,consistency in effects and economy in cost.The mixture k-ε turbulence model and the Eulerian multi-fluid model are used to simulate the flow and phase distribution in the combined T junctions.The effects of structural parameters such as the branched pipe interval and height on the flow distribution and the separation behaviors are studied.The results show that the combined T junctions under fixed inlet and outlet boundary conditions form a single hydraulic equilibrium system in which the fluid energy distributes freely till a balance is achieved.The split-flow promotes the separation of the immiscible oil and the water.The separation efficiency increases with the increase of the branched pipe interval and changes slightly with the increase of the branched pipe height.The structural change of the combined T junctions may change the flow direction in the branched pipes.Simulation results can provide some guidance for the design of the combined T junctions as one kind of oil-water separator.

  9. Flow-parametric regulation of shear-driven phase separation in two and three dimensions

    CERN Document Server

    O'Naraigh, Lennon; Naso, Aurore

    2014-01-01

    The Cahn--Hilliard equation with an externally-prescribed chaotic shear flow is studied in two and three dimensions. The flow is parametrized by its amplitudes (thereby admitting the possibility of anisotropy), lengthscales, and multiple time scales. Two key features emerge. First, for long flow correlation times, large flow amplitudes and small Cahn--Hilliard diffusivities, the phase separation and the associated coarsening phenomenon are not only arrested but in fact the concentration variance decays, thereby opening up the possibility of describing the dynamics of the concentration field using the theories of advection diffusion. Secondly, for anisotropic scenarios wherein the variance saturates, the direction in which the domains align depends on the flow correlation time. Thus, for correlation times comparable to the inverse of the mean shear rate, the domains align in the direction of maximum flow amplitude, while for short correlation times, the domains initially align in the opposite direction. Howeve...

  10. Analysis and testing the performance of a centrifugal two phase flow separator

    Energy Technology Data Exchange (ETDEWEB)

    Mirza-Moghadam, A V

    1979-01-01

    Analysis and testing the performance of an 8 in., 1.72 ft high centrifugal cyclone separator for flows up to 4.0 lbs/s and pressures ranging from 10 to 60 psig. Conclusions drawn are based on inlet steam qualities of 23 to 27 percent (x% = m/sub s//m /sub t/) .99% and better steam quality is achieved up to 3 lbs/s under 50 and 60 psig. Breakdown flow rate is found to be a linear function of separator pressure.

  11. Turbulent Heat Transfer Characteristics in the Shear Layer of a Separated Flow

    Science.gov (United States)

    Jovic, S.; Kutler, Paul F. (Technical Monitor)

    1994-01-01

    Experiments were performed to study the evolution of the heat transfer structure in a separated free shear layer region of an incompressible separated turbulent boundary layer flow behind a backward-facing step. While there is an abundance of velocity field measurements of separated flows, heat transfer measurements are rather scarce, thus limiting assessment of the heat transfer physics and its accurate modeling. The purpose of the paper is twofold: to improve an understanding of effects of flow separation on heat transfer characteristics, and to provide data for turbulence modeling and computation. The boundary layer upstream of the step was turbulent and fully developed. A constant temperature surface boundary condition was imposed upstream and downstream of the step for the heat transfer study. An internal mixing-layer like flow forms and grows from the step lip within the original boundary layer. The turbulent structure of the flow evolving downstream, however, does not switch immediately to that of a mixing layer over the entire shear layer thickness. Measurements of mean and fluctuating velocity and temperature fields indicate that the internal layer spreads gradually in the transverse direction while the outer part of the original boundary layer is effectively unperturbed. The results in this paper have not been previously reported.

  12. Flow separation control on swept wing with nanosecond pulse driven DBD plasma actuators

    Directory of Open Access Journals (Sweden)

    Zhao Guangyin

    2015-04-01

    Full Text Available A 15° swept wing with dielectric barrier discharge plasma actuator is designed. Experimental study of flow separation control with nanosecond pulsed plasma actuation is performed at flow velocity up to 40 m/s. The effects of the actuation frequency and voltage on the aerodynamic performance of the swept wing are evaluated by the balanced force and pressure measurements in the wind tunnel. At last, the performances on separation flow control of the three types of actuators with plane and saw-toothed exposed electrodes are compared. The optimal actuation frequency for the flow separation control on the swept wing is detected, namely the reduced frequency is 0.775, which is different from 2-D airfoil separation control. There exists a threshold voltage for the low swept wing flow control. Before the threshold voltage, as the actuation voltage increases, the control effects become better. The maximum lift is increased by 23.1% with the drag decreased by 22.4% at 14°, compared with the base line. However, the best effects are obtained on actuator with plane exposed electrode in the low-speed experiment and the abilities of saw-toothed actuators are expected to be verified under high-speed conditions.

  13. Lattice Boltzmann simulation of separation phenomenon in a binary gaseous flow through a microchannel

    CERN Document Server

    Wang, Liang; Guo, Zhaoli

    2016-01-01

    Gas separation of a binary gaseous mixture is one of characteristic phenomena in the micro-scale flows that differ from the conventional size flows. In this work, the separation in a binary gas mixture flows through a microchannel is investigated by the lattice Boltzmann method with a diffuse-bounce-back (DBB) boundary condition. The separation degree and rate are measured in the He--Ar and Ne--Ar systems for different mole fractions, pressure ratios, and Knudsen numbers. The results show that the separation phenomenon in the He--Ar mixture is more obvious than that in the Ne--Ar mixture at the same mole fraction owing to the larger molecular mass ratio. In addition, the increase in the pressure ratio reduces the difference in the molecular velocities between the two species, and the separation phenomenon becomes weaker. However, the gas separation is enhanced with an increase in the Knudsen number. This is because the resulting rarefaction effect reduces the interactions between the gas molecules of the two ...

  14. DSMC computations of hypersonic flow separation and re-attachment in the transition to continuum regime

    Science.gov (United States)

    Prakash, Ram; Gai, Sudhir L.; O'Byrne, Sean; Brown, Melrose

    2016-11-01

    The flow over a `tick' shaped configuration is performed using two Direct Simulation Monte Carlo codes: the DS2V code of Bird and the code from Sandia National Laboratory, called SPARTA. The configuration creates a flow field, where the flow is expanded initially but then is affected by the adverse pressure gradient induced by a compression surface. The flow field is challenging in the sense that the full flow domain is comprised of localized areas spanning continuum and transitional regimes. The present work focuses on the capability of SPARTA to model such flow conditions and also towards a comparative evaluation with results from DS2V. An extensive grid adaptation study is performed using both the codes on a model with a sharp leading edge and the converged results are then compared. The computational predictions are evaluated in terms of surface parameters such as heat flux, shear stress, pressure and velocity slip. SPARTA consistently predicts higher values for these surface properties. The skin friction predictions of both the codes don't give any indication of separation but the velocity slip plots indicate an incipient separation behavior at the corner. The differences in the results are attributed towards the flow resolution at the leading edge that dictates the downstream flow characteristics.

  15. The Two-Phase Flow Separator Experiment Breadboard Model: Reduced Gravity Aircraft Results

    Science.gov (United States)

    Rame, E; Sharp, L. M.; Chahine, G.; Kamotani, Y.; Gotti, D.; Owens, J.; Gilkey, K.; Pham, N.

    2015-01-01

    Life support systems in space depend on the ability to effectively separate gas from liquid. Passive cyclonic phase separators use the centripetal acceleration of a rotating gas-liquid mixture to carry out phase separation. The gas migrates to the center, while gas-free liquid may be withdrawn from one of the end plates. We have designed, constructed and tested a breadboard that accommodates the test sections of two independent principal investigators and satisfies their respective requirements, including flow rates, pressure and video diagnostics. The breadboard was flown in the NASA low-gravity airplane in order to test the system performance and design under reduced gravity conditions.

  16. Numerical study on flow separation in 90° pipe bend under high Reynolds number by k-ε modelling

    Directory of Open Access Journals (Sweden)

    Prasun Dutta

    2016-06-01

    Full Text Available The present paper makes an effort to find the flow separation characteristics under high Reynolds number in pipe bends. Single phase turbulent flow through pipe bends is investigated using k-ε turbulence model. After the validation of present model against existing experimental results, a detailed study has been performed to study the influence of Reynolds number on flow separation and reattachment. The separation region and the velocity field of the primary and the secondary flows in different sections have been illustrated. Numerical results show that flow separation can be clearly visualized for bend with low curvature ratio. Distributions of the velocity vector show the secondary motion clearly induced by the movement of fluid from inner to outer wall of the bend leading to flow separation. This paper provides numerical results to understand the flow characteristics of fluid flow in 90° bend pipe.

  17. A different approach on the onset of separation in the flow around a circular cylinder

    Science.gov (United States)

    Malamataris, Nikolaos; Sarris, I.; Pazis, D.; Liakos, A.

    2016-11-01

    The onset of separation in the flow around a cylinder is revisited with new insight. The goal of the research is to compute the smallest Reynolds number where the separation actual occurs rather than computing small eddies and extrapolating to the value of the Reynolds number where separation may occur. To this purpose, an accurate home made code is designed with Galerkin finite elements. The computational domain is chosen as the laboratory experiments by Taneda. It is found that in all six different choices of Taneda's diameters of the cylinders he used, separation is not observed for Re research is the computation of the drag coefficient for Reynolds numbers starting from 1 .10-5 up to 40. In addition, the separation angle (point where vorticity changes sign) is computed for 6 . 14 research aims to be the most thorough work done on that subject so far.

  18. Plasma Influence on Characteristics of Aerodynamic Friction and Separation Flow Location

    Science.gov (United States)

    2007-11-02

    Mirror- galvanometer oscillograph NO43.1 (6); • · Shadow Schlieren device IAB-451 (7-10). After a modernization the oscilloscope “Tektronix TDS...amplifier is transmitted to the mirror- galvanometer oscillograph (6), which in turn records the pressure variation diagram on a Plasma Aerodynamics...balance (4) is used, the signal from which is also transmitted to the 8-ANCh amplifier and then to the mirror- galvanometer oscillograph and is

  19. Numerical simulation of the deterministic vector separation of particles flowing over slanted open cavities

    Science.gov (United States)

    Shaqfeh, Eric S. G.; Bernate, Jorge A.; Yang, Mengfei

    2016-12-01

    Within the past decade, the separation of particles via continuous flow through microfluidic devices has been developed largely through an Edisonian approach whereby devices have been developed based on observation and intuition. This is particularly true in the development of vector chromatography at vanishingly small Reynolds number for non-Brownian particles. Note that this latter phenomenon has its origins in the irreversible forces that are at work in the device, since Stokes flow reversibility typically prohibits their function otherwise. We present a numerical simulation of the vector separation of non-Brownian particles of different sizes and deformabilities in the Stokes flow through channels whose lower surface is composed of slanted cavities. The simulations are designed to understand the physical principles behind the separation as well as to provide design criteria for devices for separating particles in a given size and flexibility range. The numerical simulations are Stokes flow boundary element simulations using techniques defined elsewhere in the literature, but including a close-range repulsive force between the particles and the slanted cavities. We demonstrate that over a range of repulsive force that is comparable to the roughness in the experimental devices, the separation data (particularly in particle size) are predicted quantitatively and are a very weak function of the range of the force. We then vary the geometric parameters of the simulated devices to demonstrate the sensitivity of the separation efficiency to these parameters, thus making design predictions as to which devices are appropriate for separating particles in different size, shape, and deformability ranges.

  20. Base flow separation: A comparison of analytical and mass balance methods

    Science.gov (United States)

    Lott, Darline A.; Stewart, Mark T.

    2016-04-01

    Base flow is the ground water contribution to stream flow. Many activities, such as water resource management, calibrating hydrological and climate models, and studies of basin hydrology, require good estimates of base flow. The base flow component of stream flow is usually determined by separating a stream hydrograph into two components, base flow and runoff. Analytical methods, mathematical functions or algorithms used to calculate base flow directly from discharge, are the most widely used base flow separation methods and are often used without calibration to basin or gage-specific parameters other than basin area. In this study, six analytical methods are compared to a mass balance method, the conductivity mass-balance (CMB) method. The base flow index (BFI) values for 35 stream gages are obtained from each of the seven methods with each gage having at least two consecutive years of specific conductance data and 30 years of continuous discharge data. BFI is cumulative base flow divided by cumulative total discharge over the period of record of analysis. The BFI value is dimensionless, and always varies from 0 to 1. Areas of basins used in this study range from 27 km2 to 68,117 km2. BFI was first determined for the uncalibrated analytical methods. The parameters of each analytical method were then calibrated to produce BFI values as close to the CMB derived BFI values as possible. One of the methods, the power function (aQb + cQ) method, is inherently calibrated and was not recalibrated. The uncalibrated analytical methods have an average correlation coefficient of 0.43 when compared to CMB-derived values, and an average correlation coefficient of 0.93 when calibrated with the CMB method. Once calibrated, the analytical methods can closely reproduce the base flow values of a mass balance method. Therefore, it is recommended that analytical methods be calibrated against tracer or mass balance methods.

  1. Experimental determination of convective heat transfer coefficients in the separated flow region of the Space Shuttle Solid Rocket Motor

    Science.gov (United States)

    Whitesides, R. Harold; Majumdar, Alok K.; Jenkins, Susan L.; Bacchus, David L.

    1990-01-01

    A series of cold flow heat transfer tests was conducted with a 7.5-percent scale model of the Space Shuttle Rocket Motor (SRM) to measure the heat transfer coefficients in the separated flow region around the nose of the submerged nozzle. Modifications were made to an existing 7.5 percent scale model of the internal geometry of the aft end of the SRM, including the gimballed nozzle in order to accomplish the measurements. The model nozzle nose was fitted with a stainless steel shell with numerous thermocouples welded to the backside of the thin wall. A transient 'thin skin' experimental technique was used to measure the local heat transfer coefficients. The effects of Reynolds number, nozzle gimbal angle, and model location were correlated with a Stanton number versus Reynolds number correlation which may be used to determine the convective heating rates for the full scale Space Shuttle Solid Rocket Motor nozzle.

  2. Effects of Whistling on Temperature Separation in a Swirling Flow-Field : 1st Report : Experiments

    OpenAIRE

    鈴木, 允; 川橋, 正昭; 吉沢, 敦朋

    1985-01-01

    The total temperature separation of gas is produced by a swirling flow-field in a circular tube. This device is called the vortex-tube or the Ranque-Hilsch tube. When whistling is produced within the swirling flow in the vortex-tube, the tangential velocity distribution in the radial direction is deformed toward the forced vortex type in the whole region. This is caused by the acoustic streaming induced by the whistling. The whistling in the vortex-tube of a counter-flow type is produced by t...

  3. LARGE-EDDY AND DETACHED-EDDY SIMULATIONS OF THE SEPARATED FLOW AROUND A CIRCULAR CYLINDER

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The separated turbulent flow around a circular cylinder is investigated using Large-Eddy Simulation (LES), Detached-Eddy Simulation (DES, or hybrid RANS/LES methods), and Unsteady Reynolds-Averaged Navier-Stokes (URANS). The purpose of this study is to examine some typical simulation approaches for the prediction of complex separated turbulent flow and to clarify the capability of applying these approaches to a typical case of the separated turbulent flow around a circular cylinder. Several turbulence models, I.e. Dynamic Sub-grid Scale (SGS) model in LES, the DES-based Spalart-Allmaras (S-A) and Shear-Stress- Transport (SST) models in DES, and the S-A and SST models in URANS, are used in the calculations. Some typical results, e.g., the mean pressure and drag coefficients, velocity profiles, Strouhal number, and Reynolds stresses, are obtained and compared with previous computational and experimental data. Based on our extensive calculations, we assess the capability and performance of these simulation approaches coupled with the relevant turbulence models to predict the separated turbulent flow.

  4. Lavrent'ev problem for separated flows with an external perturbation

    Directory of Open Access Journals (Sweden)

    Dmitriy K. Potapov

    2013-11-01

    Full Text Available We study the Lavrent'ev mathematical model for separated flows with an external perturbation. This model consists of a differential equation with discontinuous nonlinearity and a boundary condition. Using a variational method, we show the existence of a semiregular solution. As a particular case, we study the one-dimensional model.

  5. A Pressure Controlled Pinched Flow Fractionation Device for Continuous Particle Separation

    DEFF Research Database (Denmark)

    Christiansen, Thomas Lehrmann; Trosborg, Jacqueline; Tanzi, Simone;

    2012-01-01

    In this work the problem of separating small particles of di↵erent sizes is solved by developing a simple microfluidic device using pinched flow fractionation (PFF), a technique originally presented by Yamada et al. in 2004 [1]. The present work takes the concept of PFF to the next level by makin...

  6. Efficiency of size-dependent particle separation by pinched flow fractionation

    CERN Document Server

    Srivastav, Aparna; Coupier, Gwennou

    2012-01-01

    Pinched flow fractionation is shown to be an efficient and selective way to quickly separate particles by size in a very polydisperse semi-concentrated suspension. In an effort to optimize the method, we discuss the quantitative influence of the pinching intensity in the balance between the requirements of selectivity and minimal dilution.

  7. Solids removal from a coldwater recirculating system - comparison of swirl separator and radial-flow settlers

    Science.gov (United States)

    Solids removal across two settling devices, i.e., a swirl separator and a radial-flow settler, and across a microscreen drum filter was evaluated in a fully recirculating system containing a single 150 m3 'Cornell-type' dual-drain tank during the production of food-size Arctic char and rainbow trout...

  8. Separation of flow from chiral magnetic effect in U+U collisions using spectator asymmetry

    CERN Document Server

    Chatterjee, Sandeep

    2014-01-01

    We demonstrate that the prolate shape of the Uranium nucleus generates anti-correlation between spectator asymmetry and initial state ellipticity of the collision zone, providing a way to constrain the initial event shape in U+U collisions. As an application, we show that this can be used to separate the background contribution due to flow from the signals of chiral magnetic effect.

  9. Variation of flow separation over large bedforms during a tidal cycle

    DEFF Research Database (Denmark)

    Lefebvre, A.; Ferret, Y.; Paarlberg, A.J.;

    2013-01-01

    -oriented bedforms provides high-resolution bathymetry and velocity measurements that are used to simulate the hydrodynamics structure during a tidal cycle using the Delft3D numerical model. During the ebb, a large flow separation zone occurs on the steep lee side (14 to 23°) of each bedform. During the flood...

  10. Experimental and Numerical Study on the Resistance Performance of an Axial Flow Cyclone Separator

    Directory of Open Access Journals (Sweden)

    Yigang Luan

    2015-01-01

    Full Text Available The purpose of this paper is to study the pressure drop of an axial flow cyclone separator as a function of inlet velocities using experimental and computational fluid dynamics (CFD methods. First, the resistance performance of the separator was acquired under ambient pressure and temperature with little change by wind tunnel experiments. Then, numerical simulations were carried out in CFD code Fluent 6.3 under standard operating conditions. A comparison between the experimental and CFD data demonstrates that the CFD method can predict the pressure drop of the axial cyclone separator excellently. Additionally, the results show that the axial flow cyclone separators have a pressure drop coefficient of approximately 7.5. To study the effect of ambient pressure and temperature on pressure drops, the same CFD method was employed to predict the resistance performance under various operating conditions. Then the numerical results were compared with the data of a normalization process method of pressure drops raised in this paper. Their comparison demonstrated that the normalization method had a high precision in predicting the influence of ambient operating parameters on pressure drops of an axial flow cyclone separator.

  11. Flow Cytometry Sorting to Separate Viable Giant Viruses from Amoeba Co-culture Supernatants

    Science.gov (United States)

    Khalil, Jacques Y. B.; Langlois, Thierry; Andreani, Julien; Sorraing, Jean-Marc; Raoult, Didier; Camoin, Laurence; La Scola, Bernard

    2017-01-01

    Flow cytometry has contributed to virology but has faced many drawbacks concerning detection limits, due to the small size of viral particles. Nonetheless, giant viruses changed many concepts in the world of viruses, as a result of their size and hence opened up the possibility of using flow cytometry to study them. Recently, we developed a high throughput isolation of viruses using flow cytometry and protozoa co-culture. Consequently, isolating a viral mixture in the same sample became more common. Nevertheless, when one virus multiplies faster than others in the mixture, it is impossible to obtain a pure culture of the minority population. Here, we describe a robust sorting system, which can separate viable giant virus mixtures from supernatants. We tested three flow cytometry sorters by sorting artificial mixtures. Purity control was assessed by electron microscopy and molecular biology. As proof of concept, we applied the sorting system to a co-culture supernatant taken from a sample containing a viral mixture that we couldn't separate using end point dilution. In addition to isolating the quick-growing Mimivirus, we sorted and re-cultured a new, slow-growing virus, which we named “Cedratvirus.” The sorting assay presented in this paper is a powerful and versatile tool for separating viral populations from amoeba co-cultures and adding value to the new field of flow virometry. PMID:28111619

  12. Idunn Mons on Venus: Location and extent of recently active lava flows

    Science.gov (United States)

    D'Incecco, Piero; Müller, Nils; Helbert, Jörn; D'Amore, Mario

    2017-02-01

    From 2006 until 2014 the ESA Venus Express probe observed the atmosphere and surface of the Earth's twin planet. The Visible and Infrared Thermal Imaging Spectrometer (VIRTIS) has provided data that indicate the occurrence of recent volcanic activity on Venus. We selected the eastern flank of Idunn Mons - Imdr Regio's single large volcano - as the study area, since it was identified in VIRTIS data as one of the regions with relatively high values of thermal emissivity at 1 μm wavelength. Using the capabilities of specific techniques developed in the Planetary Emissivity Laboratory group at DLR in Berlin, our study intends to identify location and extent of the sources of such anomalies, thus the lava flows responsible for the relatively high emissivity observed by VIRTIS over the eastern flank of Idunn Mons. We map the lava flow units on the top and eastern flank of Idunn Mons, varying the values of simulated 1 μm emissivity assigned to the mapped units. For each configuration we calculate the total RMS error in comparison with the VIRTIS observations. In the best-fit configuration, the flank lava flows are characterized by high values of 1 μm simulated emissivity. Hence, the lava flow units on the eastern flank on Idunn Mons are likely responsible for the relatively high 1 μm emissivity anomalies observed by VIRTIS. This result is supported by the reconstructed post-eruption stratigraphy, displaying the relative dating of the mapped lava flows, that is independent of the 1 μm emissivity modeling. Values of average microwave emissivity extracted from the lava flow units range around the global mean, which is consistent with dry basalts.

  13. Model-Invariant Hybrid Computations of Separated Flows for RCA Standard Test Cases

    Science.gov (United States)

    Woodruff, Stephen

    2016-01-01

    NASA's Revolutionary Computational Aerosciences (RCA) subproject has identified several smooth-body separated flows as standard test cases to emphasize the challenge these flows present for computational methods and their importance to the aerospace community. Results of computations of two of these test cases, the NASA hump and the FAITH experiment, are presented. The computations were performed with the model-invariant hybrid LES-RANS formulation, implemented in the NASA code VULCAN-CFD. The model- invariant formulation employs gradual LES-RANS transitions and compensation for model variation to provide more accurate and efficient hybrid computations. Comparisons revealed that the LES-RANS transitions employed in these computations were sufficiently gradual that the compensating terms were unnecessary. Agreement with experiment was achieved only after reducing the turbulent viscosity to mitigate the effect of numerical dissipation. The stream-wise evolution of peak Reynolds shear stress was employed as a measure of turbulence dynamics in separated flows useful for evaluating computations.

  14. Blood plasma separation in a long two-phase plug flowing through disposable tubing.

    Science.gov (United States)

    Sun, Meng; Khan, Zeina S; Vanapalli, Siva A

    2012-12-21

    We report a simple technique to separate plasma from blood in a flowing immiscible plug. We investigate the effect of various control parameters such as blood dilution, injection flow rate, observation time and fluid properties on plasma separation. We find that the technique works best for diluted blood samples at low plug velocities and long observation times. We postulate that the main mechanism responsible for efficient separation is the sedimentation of blood cells in the plug and their subsequent collection by the moving plug causing a significant accumulation of cells at the rear of the plug. We discuss the time scales determining the sedimentation, advection and collection of a blood cell in the immiscible plug and propose a phase diagram that is able to predict the operating space for effective plasma separation. We demonstrate that the technique allows for the extraction of more than 60% of the plasma by volume from 1 μL of diluted blood. We show the practical significance of this method by compartmentalizing the separated plasma into discrete microfluidic droplets and detecting cholesterol. This technique features low consumption of blood (nL-scale) and low shear rate (∼1 s(-1)). It is inexpensive, easy to use, and has the potential to be developed as an efficient point-of-care device for blood diagnostics in resource-poor environments. More advanced applications could also be envisioned by integrating our plasma separation method into existing microfluidic drop manipulation techniques.

  15. Study of UPFC Optimal Location Considering Loss Reduction and Improvement of Voltage Stability and Power Flow

    Directory of Open Access Journals (Sweden)

    Islam Youcef DJILANI KOBIBI

    2014-02-01

    Full Text Available With the increase in power demand, operation and planning of large interconnected power system are becoming more complex, so power system will become less secure and stable. A new concept of Flexible AC Transmission system (FACTS brought radical changes in the power system operation and control. FACTS controllers narrow the gap between the no controlled and the controlled power system mode of operation, by providing additional degrees of freedom to control power flows and voltages. Unified Power Flow Controller (UPFC is a versatile FACTS device which can independently or simultaneously control the active power, the reactive power, and the bus voltage to which it is connected. The main purpose of this paper is to identify the optimal location of the Unified Power Flow Controller (UPFC in order to minimize active power losses and improve the voltage profiles using the injection model of the (UPFC in Newton-Raphson load flow algorithm, in an IEEE- 14, 30, 57, 118, 300 Bus test systems.

  16. Geophysical flows under location uncertainty, Part III: SQG and frontal dynamics under strong turbulence conditions

    CERN Document Server

    Resseguier, Valentin; Chapron, Bertrand

    2016-01-01

    Models under location uncertainty are derived assuming that a component of the velocity is uncorrelated in time. The material derivative is accordingly modified to include an advection correction, inhomogeneous and anisotropic diffusion terms and a multiplicative noise contribution. This change can be consitently applied to all fluid dynamics evolution laws. This paper continues to explore benefits of this framework and consequences of specific scaling assumptions. Starting from a Boussinesq model under location uncertainty, a model is developed to describe a mesoscale flow subject to a strong underlying submesoscale activity. As obtained, the geostrophic balance is modified and the Quasi-Geostrophic (QG) assumptions remarkably lead to a zero Potential Vorticity (PV). The ensuing Surface Quasi-Geostrophic (SQG) model provides a simple diagnosis of warm frontolysis and cold frontogenesis.

  17. On the properties and mechanisms of microjet arrays in crossflow for the control of flow separation

    Science.gov (United States)

    Fernandez, Erik J.

    By utilizing passive and active methods of flow control, the aerodynamic performance of external and internal components can be greatly improved. Recently however, the benefits of applying active flow control methods to turbomachinery components for improved fuel efficiency, reduced engine size, and greater operational envelope has sparked a renewed interest in some of these flow control techniques. The more attractive of these, is active control in the form of jets in cross flow. With their ability to be turned on and off, as well as their negligible effect on drag when not being actuated, they are well suited for applications such as compressor and turbine blades, engine inlet diffusers, internal engine passages, and general external aerodynamics. This study consists of two parts. The first is the application of active control on a low-pressure turbine (LPT) cascade to determine the effectiveness of microjet actuators on flow separation at relatively low speeds. The second study, motivated by the first, involves a parametric study on a more canonical model to examine the effects of various microjet parameters on the efficacy of separation control and to provide a better understanding of the relevant flow physics governing this control approach. With data obtained from velocity measurements across the wide parametric range, correlations for the growth of the counter-rotating vortex pairs generated by these actuators are deduced. From the information and models obtained throughout the study, basic suggestions for microjet actuator design are presented.

  18. Wavelet analysis of hemispheroid flow separation toward understanding human vocal fold pathologies

    Science.gov (United States)

    Plesniak, Daniel H.; Carr, Ian A.; Bulusu, Kartik V.; Plesniak, Michael W.

    2014-11-01

    Physiological flows observed in human vocal fold pathologies, such as polyps and nodules, can be modeled by flow over a wall-mounted protuberance. The experimental investigation of flow separation over a surface-mounted hemispheroid was performed using particle image velocimetry (PIV) and measurements of surface pressure in a low-speed wind tunnel. This study builds on the hypothesis that the signatures of vortical structures associated with flow separation are imprinted on the surface pressure distributions. Wavelet decomposition methods in one- and two-dimensions were utilized to elucidate the flow behavior. First, a complex Gaussian wavelet was used for the reconstruction of surface pressure time series from static pressure measurements acquired from ports upstream, downstream, and on the surface of the hemispheroid. This was followed by the application of a novel continuous wavelet transform algorithm (PIVlet 1.2) using a 2D-Ricker wavelet for coherent structure detection on instantaneous PIV-data. The goal of this study is to correlate phase shifts in surface pressure with Strouhal numbers associated with the vortex shedding. Ultimately, the wavelet-based analytical framework will be aimed at addressing pulsatile flows. This material is based in part upon work supported by the National Science Foundation under Grant Number CBET-1236351, and GW Center for Biomimetics and Bioinspired Engineering (COBRE).

  19. Remotely detected NMR for the characterization of flow and fast chromatographic separations using organic polymer monoliths.

    Science.gov (United States)

    Teisseyre, Thomas Z; Urban, Jiri; Halpern-Manners, Nicholas W; Chambers, Stuart D; Bajaj, Vikram S; Svec, Frantisek; Pines, Alexander

    2011-08-01

    An application of remotely detected magnetic resonance imaging is demonstrated for the characterization of flow and the detection of fast, small molecule separations within hypercrosslinked polymer monoliths. The hyper-cross-linked monoliths exhibited excellent ruggedness, with a transit time relative standard deviation of less than 2.1%, even after more than 300 column volumes were pumped through at high pressure and flow. Magnetic resonance imaging enabled high-resolution intensity and velocity-encoded images of mobile phase flow through the monolith. The images confirm that the presence of a polymer monolith within the capillary disrupts the parabolic laminar flow profile that is characteristic of mobile phase flow within an open tube. As a result, the mobile phase and analytes are equally distributed in the radial direction throughout the monolith. Also, in-line monitoring of chromatographic separations of small molecules at high flow rates is shown. The coupling of monolithic chromatography columns and NMR provides both real-time peak detection and chemical shift information for small aromatic molecules. These experiments demonstrate the unique power of magnetic resonance, both direct and remote, in studying chromatographic processes.

  20. Methicillin Resistant Staphylococcus Aureus Biofilm Formation Over A Separated Flow Region Under Steady And Pulsatile Flow Conditions

    Science.gov (United States)

    Salek, M. Mehdi; Martinuzzi, Robert

    2012-02-01

    Several researchers have observed that the formation, morphology and susceptibility of bacterial biofilms are affected by the local hydrodynamic condition and, in particular, shear stresses acting on the fluid-biofilm interface. A backwards facing step (BFS) experimental model has been widely utilized as an in vitro model to examine and characterize the effect of flow separation and recirculation zones comparable to those present within various medical devices as well as those observed in vivo. The specific geometry of BFS covers a vide range of flow features observed in physiological or environmental conditions. The hypothesis of this study is that the flow behavior and structures can effectively contribute to the transport and attachment of cells and affecting the morphology of adhered colonies as well as suspended structures (i.e. biofilm streamers). Hence, the formation of the recirculation region occurring within a backward facing step (BFS) under steady and pulsatile conditions as well as three-dimensional flow structures arising close to the side walls are investigated to correlate to biofilms behavior. This hypothesis is investigated using a backward facing step incorporated into a flow cell under steady and pulsatile flow regimes to study the growth of methicillin resistant Staphylococcus aureus (MRSA) UC18 as the study microorganism.

  1. Coherent structures and flow topology of transitional separated-reattached flow over two and three dimensional geometrical shapes

    Science.gov (United States)

    Diabil, Hayder Azeez; Li, Xin Kai; Abdalla, Ibrahim Elrayah

    2017-09-01

    Large-scale organized motions (commonly referred to coherent structures) and flow topology of a transitional separated-reattached flow have been visualised and investigated using flow visualisation techniques. Two geometrical shapes including two-dimensional flat plate with rectangular leading edge and three-dimensional square cylinder are chosen to shed a light on the flow topology and present coherent structures of the flow over these shapes. For both geometries and in the early stage of the transition, two-dimensional Kelvin-Helmholtz rolls are formed downstream of the leading edge. They are observed to be twisting around the square cylinder while they stay flat in the case of the two-dimensional flat plate. For both geometrical shapes, the two-dimensional Kelvin-Helmholtz rolls move downstream of the leading edge and they are subjected to distortion to form three-dimensional hairpin structures. The flow topology in the flat plate is different from that in the square cylinder. For the flat plate, there is a merging process by a pairing of the Kelvin-Helmholtz rolls to form a large structure that breaks down directly into many hairpin structures. For the squire cylinder case, the Kelvin-Helmholtz roll evolves topologically to form a hairpin structure. In the squire cylinder case, the reattachment length is much shorter and a forming of the three-dimensional structures is closer to the leading edge than that in the flat plate case.

  2. Comparison of different base flow separation methods in a lowland catchment

    Directory of Open Access Journals (Sweden)

    A. L. Gonzales

    2009-04-01

    Full Text Available Assessment of water resources kept in different storages and moving along different pathways in a catchment is important for its optimal use and protection, and also for the prediction of floods and low flows. Moreover, understanding of the runoff generation processes is essential for assessing the impacts of climate and land use changes on the hydrological response of a catchment. Many methods for base flow separation exist, but hardly one focuses on the specific behaviour of temperate lowland areas. This paper presents the results of a base flow separation study carried out in a lowland area in the Netherlands. In this research, field observations of precipitation, groundwater and surface water levels and discharges, together with tracer analysis are used to understand the runoff generation processes in the catchment. Several tracer and non-tracer based base flow separation methods were applied to the discharge time series, and their results are compared.

    The results show that groundwater levels react fast to precipitation events in this lowland area with shallow groundwater tables. Moreover, a good correlation was found between groundwater levels and discharges meaning that most of the measured discharge also during floods comes from the groundwater storage. It was determined using tracer hydrological approaches that approximately 90% of the total discharge is groundwater displaced by event water infiltrating in the northern part of the catchment, and only the remaining 10% is surface runoff. The impact of remote recharge causing displacement of near channel groundwater during floods could also be motivated with hydraulic approximations. The results show further that when base flow separation is meant to separate groundwater contributions to stream flow, process based methods (e.g. rating curve method; Kliner and Knezek, 1974 are more reliable than other simple non-tracer based methods. Also, the recursive filtering method (proposed

  3. Computational Flow Dynamic Simulation of Micro Flow Field Characteristics Drainage Device Used in the Process of Oil-Water Separation

    Directory of Open Access Journals (Sweden)

    Guangya Jin

    2017-01-01

    Full Text Available Aqueous crude oil often contains large amounts of produced water and heavy sediment, which seriously threats the safety of crude oil storage and transportation. Therefore, the proper design of crude oil tank drainage device is prerequisite for efficient purification of aqueous crude oil. In this work, the composition and physicochemical properties of crude oil samples were tested under the actual conditions encountered. Based on these data, an appropriate crude oil tank drainage device was developed using the principle of floating ball and multiphase flow. In addition, the flow field characteristics in the device were simulated and the contours and streamtraces of velocity magnitude at different nine moments were obtained. Meanwhile, the improvement of flow field characteristics after the addition of grids in crude oil tank drainage device was validated. These findings provide insights into the development of effective selection methods and serve as important references for oil-water separation process.

  4. In situ cardiac perfusion reveals interspecific variation of intraventricular flow separation in reptiles.

    Science.gov (United States)

    Joyce, William; Axelsson, Michael; Altimiras, Jordi; Wang, Tobias

    2016-07-15

    The ventricles of non-crocodilian reptiles are incompletely divided and provide an opportunity for mixing of oxygen-poor blood and oxygen-rich blood (intracardiac shunting). However, both cardiac morphology and in vivo shunting patterns exhibit considerable interspecific variation within reptiles. In the present study, we develop an in situ double-perfused heart approach to characterise the propensity and capacity for shunting in five reptile species: the turtle Trachemys scripta, the rock python Python sebae, the yellow anaconda Eunectes notaeus, the varanid lizard Varanus exanthematicus and the bearded dragon Pogona vitticeps To simulate changes in vascular bed resistance, pulmonary and systemic afterloads were independently manipulated and changes in blood flow distribution amongst the central outflow tracts were monitored. As previously demonstrated in Burmese pythons, rock pythons and varanid lizards exhibited pronounced intraventricular flow separation. As pulmonary or systemic afterload was raised, flow in the respective circulation decreased. However, flow in the other circulation, where afterload was constant, remained stable. This correlates with the convergent evolution of intraventricular pressure separation and the large intraventricular muscular ridge, which compartmentalises the ventricle, in these species. Conversely, in the three other species, the pulmonary and systemic flows were strongly mutually dependent, such that the decrease in pulmonary flow in response to elevated pulmonary afterload resulted in redistribution of perfusate to the systemic circuit (and vice versa). Thus, in these species, the muscular ridge appeared labile and blood could readily transverse the intraventricular cava. We conclude that relatively minor structural differences between non-crocodilian reptiles result in the fundamental changes in cardiac function. Further, our study emphasises that functionally similar intracardiac flow separation evolved independently in

  5. Spontaneous reconnection at a separator current layer: 2. Nature of the waves and flows

    Science.gov (United States)

    Stevenson, J. E. H.; Parnell, C. E.

    2015-12-01

    Sudden destabilizations of the magnetic field, such as those caused by spontaneous reconnection, will produce waves and/or flows. Here we investigate the nature of the plasma motions resulting from spontaneous reconnection at a 3-D separator. In order to clearly see these perturbations, we start from a magnetohydrostatic equilibrium containing two oppositely signed null points joined by a generic separator along which lies a twisted current layer. The nature of the magnetic reconnection initiated in this equilibrium as a result of an anomalous diffusivity is discussed in detail in Stevenson and Parnell (2015). The resulting sudden loss of force balance inevitably generates waves that propagate away from the diffusion region carrying the dissipated current. In their wake a twisting stagnation flow, in planes perpendicular to the separator, feeds flux back into the original diffusion site (the separator) in order to try to regain equilibrium. This flow drives a phase of slow weak impulsive bursty reconnection that follows on after the initial fast-reconnection phase.

  6. Continuous particle separation using pressure-driven flow-induced miniaturizing free-flow electrophoresis (PDF-induced μ-FFE).

    Science.gov (United States)

    Jeon, Hyungkook; Kim, Youngkyu; Lim, Geunbae

    2016-01-28

    In this paper, we introduce pressure-driven flow-induced miniaturizing free-flow electrophoresis (PDF-induced μ-FFE), a novel continuous separation method. In our separation system, the external flow and electric field are applied to particles, such that particle movement is affected by pressure-driven flow, electroosmosis, and electrophoresis. We then analyzed the hydrodynamic drag force and electrophoretic force applied to the particles in opposite directions. Based on this analysis, micro- and nano-sized particles were separated according to their electrophoretic mobilities with high separation efficiency. Because the separation can be achieved in a simple T-shaped microchannel, without the use of internal electrodes, it offers the advantages of low-cost, simple device fabrication and bubble-free operation, compared with conventional μ-FFE methods. Therefore, we expect the proposed separation method to have a wide range of filtering/separation applications in biochemical analysis.

  7. Development of flow separation control system to reduce the vibration of wind turbine blades

    Science.gov (United States)

    Kim, Ho-Young; Kim, Ho-Hyun; Han, Jong-Seob; Han, Jae-Hung

    2017-04-01

    The size of wind turbine blade has been continuously increased. Large-scale wind turbine blades induce loud noise, vibration; and maintenance difficulty is also increased. It causes the eventual increases of the cost of energy. The vibration of wind turbine blade is caused by several reasons such as a blade rotation, tower shadow, wind shear, and flow separation of a wind turbine blade. This wind speed variation changes in local angle of attack of the blades and create the vibration. The variation of local angle of attack influences the lift coefficient and causes the large change of the lift. In this study, we focus on the lift coefficient control using a flow control device to reduce the vibration. DU35-A15 airfoil was employed as baseline model. A plasma actuator was installed to generate the upwind jet in order to control the lift coefficient. Wind tunnel experiment was performed to demonstrate of the performance of the plasma actuator. The results show the plasma actuator can induce the flow separation compared with the baseline model. In addition, the actuator can delay the flow separation depending on the input AC frequency with the same actuator configuration.

  8. Capillary flow of blood in a microchannel with differential wetting for blood plasma separation and on-chip glucose detection.

    Science.gov (United States)

    Maria, M Sneha; Rakesh, P E; Chandra, T S; Sen, A K

    2016-09-01

    We report capillary flow of blood in a microchannel with differential wetting for the separation of a plasma from sample blood and subsequent on-chip detection of glucose present in a plasma. A rectangular polydimethylsiloxane microchannel with hydrophilic walls (on three sides) achieved by using oxygen plasma exposure enables capillary flow of blood introduced at the device inlet through the microchannel. A hydrophobic region (on all four sides) in the microchannel impedes the flow of sample blood, and the accumulated blood cells at the region form a filter to facilitate the separation of a plasma. The modified wetting property of the walls and hence the device performance could be retained for a few weeks by covering the channels with deionised water. The effects of the channel cross-section, exposure time, waiting time, and location and length of the hydrophobic region on the volume of the collected plasma are studied. Using a channel cross-section of 1000 × 400 μm, an exposure time of 2 min, a waiting time of 10 min, and a hydrophobic region of width 1.0 cm located at 10 mm from the device inlet, 450 nl of plasma was obtained within 15 min. The performance of the device was found to be unaffected (provides 450 nl of plasma in 15 min) even after 15 days. The purification efficiency and plasma recovery of the device were measured and found to be comparable with that obtained using the conventional centrifugation process. Detection of glucose at different concentrations in whole blood of normal and diabetic patients was performed (using 5 μl of sample blood within 15 min) to demonstrate the compatibility of the device with integrated detection modules.

  9. Comparison of different base flow separation methods in a lowland catchment

    Directory of Open Access Journals (Sweden)

    S. Uhlenbrook

    2009-11-01

    Full Text Available Assessment of water resources available in different storages and moving along different pathways in a catchment is important for its optimal use and protection, and also for the prediction of floods and low flows. Moreover, understanding of the runoff generation processes is essential for assessing the impacts of climate and land use changes on the hydrological response of a catchment. Many methods for base flow separation exist, but hardly one focuses on the specific behaviour of temperate lowland areas. This paper presents the results of a base flow separation study carried out in a lowland area in the Netherlands. In this study, field observations of precipitation, groundwater and surface water levels and discharges, together with tracer analysis are used to understand the runoff generation processes in the catchment. Several tracer and non-tracer based base flow separation methods were applied to the discharge time series, and their results are compared.

    The results show that groundwater levels react fast to precipitation events in this lowland area with shallow groundwater tables. Moreover, a good correlation was found between groundwater levels and discharges suggesting that most of the measured discharge also during floods comes from groundwater storage. It was estimated using tracer hydrological approaches that approximately 90% of the total discharge is groundwater displaced by event water mainly infiltrating in the northern part of the catchment, and only the remaining 10% is surface runoff. The impact of remote recharge causing displacement of near channel groundwater during floods could also be motivated with hydraulic approximations. The results show further that when base flow separation is meant to identify groundwater contributions to stream flow, process based methods (e.g. the rating curve method; Kliner and Knezek, 1974 are more reliable than other simple non-tracer based methods. Also, the recursive filtering method

  10. Enhancing separation of histidine from amino acids via free-flow affinity electrophoresis with gravity-induced uniform hydrodynamic flow.

    Science.gov (United States)

    Pang, Bo; Shao, Jing; Zhang, Jie; Geng, Jia-Zhen; Fan, Liu-Yin; Cao, Cheng-Xi; Hou, Jing-Li

    2012-03-01

    In this paper, a novel mode of free-flow affinity electrophoresis (FFAE) was developed to indirectly enhance the separation of free-flow electrophoresis (FFE). In the mode of FFAE, a Ni(II) with high electric charge density and histidine (His) is chosen as a model ligand and target solute, respectively. Through the controlling of experimental conditions (10 mM pH 6.0 Na(2)HPO(4)-NaH(2)PO(4) with 2.0 mM NiCl(2)·6H(2)O background buffer), Ni(II) can combine with His and the combination leads to the high electric charge density of affinity complex of His-Ni(II) in contrast to the low density of free His molecule. But the ligand has weak interaction with uninterested amino acids. Thus, the mobility of His existing as His-Ni(II) is greatly increased from 14.5×10(-8) m(2) V(-1) s(-1) to 30.2 × 10(-8) m(2) V(-1) s(-1), while those mobilities of uninterested amino acids are almost constant. By virtue of the mode, we developed the FFAE procedure and conducted the relevant experiments. The experiments demonstrated the following merits of the FFAE technique: (i) clear enhancement of separation between the target solute of His and uninterested amino acids; (ii) simplicity, and (iii) low cost. Furthermore, the technique was used for the continuous separation of His from its complex sample, and the purity of His was near to 100%. All of the results demonstrate the feasibility of affinity separation in FFE. The developed FFAE may be used in the separation and pretreatment of some biological molecules (e.g. peptides).

  11. Low-Frequency Flow Oscillations on Stalled Wings Exhibiting Cellular Separation Topology

    Science.gov (United States)

    Disotell, Kevin James

    One of the most pervasive threats to aircraft controllability is wing stall, a condition associated with loss of lift due to separation of air flow from the wing surface at high angles of attack. A recognized need for improved upset recovery training in extended-envelope flight simulators is a physical understanding of the post-stall aerodynamic environment, particularly key flow phenomena which influence the vehicle trajectory. Large-scale flow structures known as stall cells, which scale with the wing chord and are spatially-periodic along the span, have been previously observed on post-stall airfoils with trailing-edge separation present. Despite extensive documentation of stall cells in the literature, the physical mechanisms behind their formation and evolution have proven to be elusive. The undertaken study has sought to characterize the inherently turbulent separated flow existing above the wing surface with cell formation present. In particular, the question of how the unsteady separated flow may interact with the wing to produce time-averaged cellular surface patterns is considered. Time-resolved, two-component particle image velocimetry measurements were acquired at the plane of symmetry of a single stall cell formed on an extruded NACA 0015 airfoil model at chord Reynolds number of 560,000 to obtain insight into the time-dependent flow structure. The evolution of flow unsteadiness was analyzed over a static angle-of-attack range covering the narrow post-stall regime in which stall cells have been observed. Spectral analysis of velocity fields acquired near the stall angle confirmed a low-frequency flow oscillation previously detected in pointwise surface measurements by Yon and Katz (1998), corresponding to a Strouhal number of 0.042 based on frontal projected chord height. Probability density functions of the streamwise velocity component were used to estimate the convective speed of this mode at approximately half the free-stream velocity, in agreement

  12. Machine Learning-Assisted Predictions of Turbulent Separated Flows over Airfoils

    Science.gov (United States)

    Singh, Anand Pratap; Medida, Shivaji; Duraisamy, Karthik

    2016-11-01

    RANS based models are typically found to be lacking in predictive accuracy when applied to complex flows, particularly those involving adverse pressure gradients and flow separation. A modeling paradigm is developed to effectively augment turbulence models by utilizing limited data (such as surface pressures and lift) from physical experiments. The key ingredients of our approach involve Inverse modeling to infer the spatial distribution of model discrepancies, and Neural networks to reconstruct discrepancy information from a large number of inverse problems into corrective model forms. Specifically, we apply the methodology to turbulent flows over airfoils involving flow separation. When the machine learning-generated model forms are embedded within a standard solver setting, we show that much improved predictions can be achieved, even in geometries and flow conditions that were not used in model training. The usage of very limited data (such as the measured lift coefficient) as an input to construct comprehensive model corrections provides a renewed perspective towards the use of vast, but sparse, amounts of available experimental datasets towards the end of developing predictive turbulence models. This work was funded by the NASA Aeronautics Research Institute (NARI) under the Leading Edge Aeronautics Research for NASA (LEARN) program with Gary Coleman as the technical monitor.

  13. Mathematical modeling and simulation of biologically inspired hair receptor arrays in laminar unsteady flow separation

    Science.gov (United States)

    Dickinson, B. T.; Singler, J. R.; Batten, B. A.

    2012-02-01

    Bats possess arrays of distributed flow-sensitive hair-like mechanoreceptors on their dorsal and ventral wing surfaces. Bat wing hair receptors are known to play a significant role in flight maneuverability and are directionally most sensitive to reversed flow over the wing. In this work, we consider the mechanics of flexible hair-like structures for the time accurate detection and visualization of hydrodynamic images associated with unsteady near surface flow phenomena. A nonlinear viscoelastic model of a hair-like structure coupled to an unsteady nonuniform flow is proposed. Writing the hair model in nondimensional form, we identify five dimensionless groups that govern hair behavior. An order of magnitude analysis of the physical forces involved in the fluid-structure hair response is performed. Through the choice of hair material properties, we show how a local measure of near surface flow velocity may be obtained from hair tip displacement and resultant moment. When hair structures are placed into an array, time and space accurate hydrodynamic images may be obtained. We illustrate the imaging of reversed flow that occurs during a laminar unsteady flow separation with an array of hair-like structures.

  14. The use of a polymer inclusion membrane for separation and preconcentration of orthophosphate in flow analysis.

    Science.gov (United States)

    Nagul, Edward A; Fontàs, Clàudia; McKelvie, Ian D; Cattrall, Robert W; Kolev, Spas D

    2013-11-25

    A highly sensitive flow analysis system has been developed for the trace determination of reactive phosphate in natural waters, which uses a polymer inclusion membrane (PIM) with Aliquat 336 as the carrier for on-line analyte separation and preconcentration. The system operates under flow injection (FI) and continuous flow (CF) conditions. Under optimal FI conditions the system is characterised by a linear concentration range between 0.5 and 1000 μg L(-1)P, a sampling rate of 10h(-1), a limit of detection of 0.5 μgL(-1)P and RSDs of 3.2% (n = 10, 100 μg L(-1)) and 7.7% (n = 10, 10 μg L(-1)). Under CF conditions with 10 min stop-flow time and sample solution flow rate of 1.32 mL min(-1) the flow system offers a limit of detection of 0.04 μg L(-1)P, a sampling rate of 5h(-1) and an RSD of 3.4% (n=5, 2.0 μg L(-1)). Interference studies revealed that anions commonly found in natural waters did not interfere when in excess of at least one order of magnitude. The flow system, operating under CF conditions, was successfully applied to the analysis of natural water samples containing concentrations of phosphate in the low μg L(-1)P range, using the multipoint standard addition method.

  15. Utilization of milli-scale coiled flow inverter in combination with phase separator for continuous flow liquid-liquid extraction processes

    NARCIS (Netherlands)

    Vural Gürsel, Iris; Kurt, Safa Kutup; Aalders, Jasper; Wang, Qi; Noël, Timothy; Nigam, Krishna D P; Kockmann, Norbert; Hessel, Volker

    2016-01-01

    Process-design intensification situated under the umbrella of Novel Process Windows heads for process integration and here most development is needed for flow separators. The vision is to achieve multi-step synthesis in flow on pilot scale. This calls for scale-up of separation units. This study is

  16. Numerical simulations of gas-liquid-solid flows in a hydrocyclone separator

    Energy Technology Data Exchange (ETDEWEB)

    Mousavian, S.M.; Najafi, A.F. [Power and Water University of Technology, School of Energy Engineering, P.O. Box: 16765-1719, Tehran (Iran)

    2009-05-15

    The flow behavior in hydrocyclones is quite complex. In this study, the computational fluid dynamics (CFD) method was used to simulate the flow fields inside a hydrocyclone in order to investigate its separation efficiency. In the computational fluid dynamics study of hydrocyclones, the air-core dimension is a key to predicting the mass split between the underflow and overflow. In turn, the mass split influences the prediction of the size classification curve. Three models, the k-{epsilon} model, the Reynolds stress model (RSM) without considering the air-core, and the Reynolds stress turbulence model with the volume of fluid (VOF) multiphase model for simulating the air-core, were compared in terms of their predictions of velocity, axial and tangential velocity distributions, and separation proportion. The RSM with air-core simulation model, since it reproduces some detailed features of the turbulence and multiphase, clearly predicted the experimental data more closely than did the other two models. (orig.)

  17. Dynamic unified RANS-LES simulations of high Reynolds number separated flows

    Science.gov (United States)

    Mokhtarpoor, Reza; Heinz, Stefan; Stoellinger, Michael

    2016-09-01

    The development of hybrid RANS-LES methods is seen to be a very promising approach to enable efficient simulations of high Reynolds number turbulent flows involving flow separation. To contribute to further advances, we present a new, theoretically well based, dynamic hybrid RANS-LES method, referred to as DLUM. It is applied to a high Reynolds number flow involving both attached and separated flow regimes: a periodic hill flow is simulated at a Reynolds number of 37 000. Its performance is compared to pure LES, pure RANS, other hybrid RANS-LES (given by DLUM modifications), and experimental observations. It is shown that the use of this computational method offers huge cost reductions (which scale with Re/200, Re refers to the Reynolds number) of very high Reynolds number flow simulations compared to LES, it is much more accurate than RANS, and more accurate than LES, which is not fully resolved. In particular, this conclusion does also apply to the comparison of DLUM and pure LES simulations on rather coarse grids, which are often simply required to deal with simulations of very high Reynolds number flows: the DLUM provides mean velocity fields which are hardly affected by the grid, whereas LES velocity fields reveal significant shortcomings. We identified the reason for the superior performance of our new dynamic hybrid RANS-LES method compared to LES: it is the model's ability to respond to a changing resolution with adequate turbulent viscosity changes by ensuring simultaneously a physically correct turbulence length scale specification under the presence of interacting RANS and LES modes.

  18. Modeling of ion transport through a porous separator in vanadium redox flow batteries

    Science.gov (United States)

    Zhou, X. L.; Zhao, T. S.; An, L.; Zeng, Y. K.; Wei, L.

    2016-09-01

    In this work, we develop a two-dimensional, transient model to investigate the mechanisms of ion-transport through a porous separator in VRFBs and their effects on battery performance. Commercial-available separators with pore sizes of around 45 nm are particularly investigated and effects of key separator design parameters and operation modes are explored. We reveal that: i) the transport mechanism of vanadium-ion crossover through available separators is predominated by convection; ii) reducing the pore size below 15 nm effectively minimizes the convection-driven vanadium-ion crossover, while further reduction in migration- and diffusion-driven vanadium-ion crossover can be achieved only when the pore size is reduced to the level close to the sizes of vanadium ions; and iii) operation modes that can affect the pressure at the separator/electrode interface, such as the electrolyte flow rate, exert a significant influence on the vanadium-ion crossover rate through the available separators, indicating that it is critically important to equalize the pressure on each half-cell of a power pack in practical applications.

  19. Heuristics methods for the flow shop scheduling problem with separated setup times

    Directory of Open Access Journals (Sweden)

    Marcelo Seido Nagano

    2012-06-01

    Full Text Available This paper deals with the permutation flow shop scheduling problem with separated machine setup times. As a result of an investigation on the problem characteristics, four heuristics methods are proposed with procedures of the construction sequencing solution by an analogy with the asymmetric traveling salesman problem with the objective of minimizing makespan. Experimental results show that one of the new heuristics methods proposed provide high quality solutions in comparisons with the evaluated methods considered in the literature.

  20. Comparative study of hybrid RANS-LES models for separated flows

    Science.gov (United States)

    Kumar, G.; Lakshmanan, S. K.; Gopalan, H.; De, A.

    2016-06-01

    Hybrid RANS-LES models are proven to be capable of predicting massively separated flows with reasonable computation cost. In this paper, Spalart-Allmaras (S-A) based detached eddy simulation (DES) model and three SST based hybrid models with different RANS to LES switching criteriaare investigated. The flow over periodic hill at Re = 10,595 is chosen as the benchmark for comparing the performance of the different models due to the complex flow physics and reasonablecomputational cost. The model performances are evaluated based on their prediction capabilities of velocity and stress profiles, and separation and reattachment point. The simulated results are validatedagainst experimental and numerical results available in literature. The S-A DES model predicted separation bubble accurately at the top of the hill, as reported earlier in experiments and other numerical results. This model also correctly predicted velocity and stress profiles in recirculation region. However, the performance of this model was poor in the post reattachment region. On the other hand, the k-ω SST based hybrid models performed poorly in recirculation region, but it fairly predicted stress profiles in post reattachment region.

  1. Circuit modification in electrical field flow fractionation systems generating higher resolution separation of nanoparticles.

    Science.gov (United States)

    Tasci, Tonguc O; Johnson, William P; Fernandez, Diego P; Manangon, Eliana; Gale, Bruce K

    2014-10-24

    Compared to other sub-techniques of field flow fractionation (FFF), cyclical electrical field flow fractionation (CyElFFF) is a relatively new method with many opportunities remaining for improvement. One of the most important limitations of this method is the separation of particles smaller than 100nm. For such small particles, the diffusion rate becomes very high, resulting in severe reductions in the CyElFFF separation efficiency. To address this limitation, we modified the electrical circuitry of the ElFFF system. In all earlier ElFFF reports, electrical power sources have been directly connected to the ElFFF channel electrodes, and no alteration has been made in the electrical circuitry of the system. In this work, by using discrete electrical components, such as resistors and diodes, we improved the effective electric field in the system to allow high resolution separations. By modifying the electrical circuitry of the ElFFF system, high resolution separations of 15 and 40nm gold nanoparticles were achieved. The effects of applying different frequencies, amplitudes and voltage shapes have been investigated and analyzed through experiments. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. Robust Face Location and Tracking Using Optical Flow and Genetic Algorithms

    Institute of Scientific and Technical Information of China (English)

    WANG Yanjiang; YUAN Baozong

    2001-01-01

    This paper presents a new and robustapproach to the detection, localization and tracking ofa human face in image sequences. First, a fast algo-rithm based on the neighbor-point-reliability is pro-posed to calculate the optical flow, which is used toextract the motion region. Then the hair and thehead knowledges are used to locate the face area. Forface tracking, a new genetic algorithms-based dynamictemplate-matching method is applied to search thenew position of the face in each new video frame. Ex-perimental results show that the proposed face track-ing method is fast and robust to illumination, faceposes, facial expressions and image distractors suchas facial occlusion by hands.

  3. Network-based representation of energy transfer in unsteady separated flow

    Science.gov (United States)

    Nair, Aditya; Taira, Kunihiko

    2015-11-01

    We construct a network-based representation of energy pathways in unsteady separated flows using a POD-Galerkin projection model. In this formulation, we regard the POD modes as the network nodes and the energy transfer between the modes as the network edges. Based on the energy transfer analysis performed by Noack et al. (2008), edge weights are characterized on the interaction graph. As an example, we examine the energy transfer within the two-dimensional incompressible flow over a circular cylinder. In particular, we analyze the energy pathways involved in flow transition from the unstable symmetric steady state to periodic shedding cycle. The growth of perturbation energy over the network is examined to highlight key features of flow physics and to determine how the energy transfer can be influenced. Furthermore, we implement closed-loop flow control on the POD-Galerkin model to alter the energy interaction path and modify the global behavior of the wake dynamics. The insights gained will be used to perform further network analysis on fluid flows with added complexity. Work supported by US Army Research Office (W911NF-14-1-0386) and US Air Force Office of Scientific Research (YIP: FA9550-13-1-0183).

  4. Three-Dimensional Flow Separation Induced by a Model Vocal Fold Polyp

    Science.gov (United States)

    Stewart, Kelley C.; Erath, Byron D.; Plesniak, Michael W.

    2012-11-01

    The fluid-structure energy exchange process for normal speech has been studied extensively, but it is not well understood for pathological conditions. Polyps and nodules, which are geometric abnormalities that form on the medial surface of the vocal folds, can disrupt vocal fold dynamics and thus can have devastating consequences on a patient's ability to communicate. A recent in-vitro investigation of a model polyp in a driven vocal fold apparatus demonstrated that such a geometric abnormality considerably disrupts the glottal jet behavior and that this flow field adjustment was a likely reason for the severe degradation of the vocal quality in patients. Understanding of the formation and propagation of vortical structures from a geometric protuberance, and their subsequent impact on the aerodynamic loadings that drive vocal fold dynamic, is a critical component in advancing the treatment of this pathological condition. The present investigation concerns the three-dimensional flow separation induced by a wall-mounted prolate hemispheroid with a 2:1 aspect ratio in cross flow, i.e. a model vocal fold polyp. Unsteady three-dimensional flow separation and its impact of the wall pressure loading are examined using skin friction line visualization and wall pressure measurements. Supported by the National Science Foundation, Grant No. CBET-1236351 and GW Center for Biomimetics and Bioinspired Engineering (COBRE).

  5. Analysis of liposomes using asymmetrical flow field-flow fractionation: separation conditions and drug/lipid recovery.

    Science.gov (United States)

    Kuntsche, Judith; Decker, Christiane; Fahr, Alfred

    2012-08-01

    Liposomes composed of dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol were analyzed by asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering. In addition to evaluation of fractionation conditions (flow conditions, sample mass, carrier liquid), radiolabeled drug-loaded liposomes were used to determine the liposome recovery and a potential loss of incorporated drug during fractionation. Neither sample concentration nor the cross-flow gradient distinctly affected the size results but at very low sample concentration (injected mass 5 μg) the fraction of larger vesicles was underestimated. Imbalance in the osmolality between the inner and outer aqueous phase resulted in liposome swelling after dilution in hypoosmotic carrier liquids. In contrast, liposome shrinking under hyperosmotic conditions was barely visible. The liposomes themselves eluted completely (lipid recoveries were close to 100%) but there was a loss of incorporated drugs during separation with a strong dependence on the octanol-water partition coefficient of the drug. Whereas corticosterone (partition coefficient ~2) was washed out more or less completely (recovery about 2%), loss of temoporfin (partition coefficient ~9) was only minor (recovery about 80%). All fractionations were well repeatable under the experimental conditions applied in the present study.

  6. Controlled reattachment in separated flows: a variational approach to recirculation length reduction

    CERN Document Server

    Boujo, E

    2014-01-01

    A variational technique is used to derive analytical expressions for the sensitivity of recirculation length to steady forcing in separated flows. Linear sensitivity analysis is applied to the two-dimensional steady flow past a circular cylinder for Reynolds numbers $40 \\leq Re \\leq 120$, both in the subcritical and supercritical regimes. Regions which are the most sensitive to volume forcing and wall blowing/suction are identified. Control configurations which reduce the recirculation length are designed based on the sensitivity information, in particular small cylinders used as control devices in the wake of the main cylinder, and fluid suction at the cylinder wall. Validation against full non-linear Navier-Stokes calculations shows excellent agreement for small-amplitude control. The linear stability properties of the controlled flow are systematically investigated. At moderate Reynolds numbers, we observe that regions where control reduces the recirculation length correspond to regions where it has a stab...

  7. Flow-background subtraction in the charge-separation measurements in heavy-ion collisions

    Science.gov (United States)

    Wen, Fufang

    2016-09-01

    Recent azimuthal-angle correlation measurements in high-energy heavy-ion collisions have observed charge-separation signals perpendicular to the reaction plane, and the observations have been related to the chiral magnetic effect (CME). However, the correlation signal is contaminated with the background contributions due to the collective motion (flow) of the collision system, and it remains elusive to effectively remove the background from the correlation. In this poster, we present a method study with a simple Monte Carlo simulation and the AMPT model. We develop a scheme to reveal the true CME signal via the event-shape engineering with the magnitude of the flow vector, Q: the flow-background is removed at Q = 0. Artificial signal/background effects will also be discussed.

  8. Polystyrene latex separations by continuous flow electrophoresis on the Space Shuttle

    Science.gov (United States)

    Snyder, R. S.; Rhodes, P. H.; Miller, T. Y.; Micale, F. J.; Mann, R. V.

    1986-01-01

    The seventh mission of the Space Shuttle carried two NASA experiments in the McDonnell Douglas Astronautics Corporation continuous flow electrophoresis system. The objectives were to test the operation of continuous flow electrophoresis in a reduced gravity environment using stable particles with established electrokinetic properties and specifically to evaluate the influence of the electrical properties of the sample constituents on the resolution of the continuous flow electrophoretic device. Polystrene latex microspheres dispersed in a solution with three times the electrical conductivity of the curtain buffer separated with a significantly larger band spread compared to the second experiment under matched conductivity conditions. It is proposed that the sample of higher electrical conductivity distorted the electric field near the sample stream so that the polystyrene latex particles migrated toward the chamber walls where electroosmosis retarded and spread the sample.

  9. Three Dimensional Separation with Spiral-Focus in a Decelerating Duct Flow (Effect of Asymmetric Inlet Boundary Layer Thickness)

    Institute of Scientific and Technical Information of China (English)

    Yoichi Kinoue; Toshiaki Setoguchi; Kenji Kaneko; Mamun Mohammad; Masahiro Inoue

    2003-01-01

    An experimental apparatus was developed to study the three dimensional separated flow with spiral-foci. The internal decelerating flow was generated by the air suction from a side wall to produce the separation on an opposite-side wall. The relation between the upstream boundary layer and the generation of spiral-foci in the separation region was observed by a tuft method. As a result, it was clarified that the spiral-focus type separation could be produced on the side wall and its behavior was closely related to the vortices supplied into the separation region from the boundary layer developing along top wall or bottom one.

  10. Control of unsteady separated flow associated with the dynamic stall of airfoils

    Science.gov (United States)

    Wilder, M. C.

    1995-01-01

    An effort to understand and control the unsteady separated flow associated with the dynamic stall of airfoils was funded for three years through the NASA cooperative agreement program. As part of this effort a substantial data base was compiled detailing the effects various parameters have on the development of the dynamic stall flow field. Parameters studied include Mach number, pitch rate, and pitch history, as well as Reynolds number (through two different model chord lengths) and the condition of the boundary layer at the leading edge of the airfoil (through application of surface roughness). It was found for free stream Mach numbers as low as 0.4 that a region of supersonic flow forms on the leading edge of the suction surface of the airfoil at moderate angles of attack. The shocks which form in this supersonic region induce boundary-layer separation and advance the dynamic stall process. Under such conditions a supercritical airfoil profile is called for to produce a flow field having a weaker leading-edge pressure gradient and no leading-edge shocks. An airfoil having an adaptive-geometry, or dynamically deformable leading edge (DDLE), is under development as a unique active flow-control device. The DDLE, formed of carbon-fiber composite and fiberglass, can be flexed between a NACA 0012 profile and a supercritical profile in a controllable fashion while the airfoil is executing an angle-of-attack pitch-up maneuver. The dynamic stall data were recorded using point diffraction interferometry (PDI), a noninvasive measurement technique. A new high-speed cinematography system was developed for recording interferometric images. The system is capable of phase-locking with the pitching airfoil motion for real-time documentation of the development of the dynamic stall flow field. Computer-aided image analysis algorithms were developed for fast and accurate reduction of the images, improving interpretation of the results.

  11. Maternal separation enhances object location memory and prevents exercise-induced MAPK/ERK signalling in adult Sprague-Dawley rats.

    Science.gov (United States)

    Makena, Nokuthula; Bugarith, Kishor; Russell, Vivienne A

    2012-09-01

    Early life stress increases the risk of developing psychopathology accompanied by reduced cognitive function in later life. Maternal separation induces anxiety-like behaviours and is associated with impaired memory. On the other hand, exercise has been shown to diminish anxiety-like behaviours and improve cognitive function. The effects of maternal separation and exercise on anxiety, memory and hippocampal proteins were investigated in male Sprague-Dawley rats. Maternal separation produced anxiety-like behaviours which were reversed by exercise. Maternal separation also enhanced object location memory which was not affected by exercise. Exercise did, however, increase synaptophysin and phospho-extracellular signal-regulated kinase (p-ERK) in the hippocampus of non-separated rats and this effect was not observed in maternally separated rats. These findings show that maternal separation selectively enhanced n memory and prevented activation of the MAPK/ERK signalling pathway in the adult rat hippocampus.

  12. Inverse design of a proper number, shapes, sizes, and locations of coolant flow passages

    Science.gov (United States)

    Dulikravich, George S.

    1992-01-01

    During the past several years we have developed an inverse method that allows a thermal cooling system designer to determine proper sizes, shapes, and locations of coolant passages (holes) in, say, an internally cooled turbine blade, a scram jet strut, a rocket chamber wall, etc. Using this method the designer can enforce a desired heat flux distribution on the hot outer surface of the object, while simultaneously enforcing desired temperature distributions on the same hot outer surface as well as on the cooled interior surfaces of each of the coolant passages. This constitutes an over-specified problem which is solved by allowing the number, sizes, locations and shapes of the holes to adjust iteratively until the final internally cooled configuration satisfies the over-specified surface thermal conditions and the governing equation for the steady temperature field. The problem is solved by minimizing an error function expressing the difference between the specified and the computed hot surface heat fluxes. The temperature field analysis was performed using our highly accurate boundary integral element code with linearly varying temperature along straight surface panels. Examples of the inverse design applied to internally cooled turbine blades and scram jet struts (coated and non-coated) having circular and non-circular coolant flow passages will be shown.

  13. Gene flow between sympatric life history forms of Oncorhynchus mykiss located above and below migratory barriers.

    Directory of Open Access Journals (Sweden)

    Donald M Van Doornik

    Full Text Available Oncorhynchus mykiss have a diverse array of life history types, and understanding the relationship among types is important for management of the species. Patterns of gene flow between sympatric freshwater resident O. mykiss, commonly known as rainbow trout, and anadromous O. mykiss, commonly known as steelhead, populations are complex and poorly understood. In this study, we attempt to determine the occurrence and pathways of gene flow and the degree of genetic similarity between sympatric resident and anadromous O. mykiss in three river systems, and investigate whether resident O. mykiss are producing anadromous offspring in these rivers, two of which have complete barriers to upstream migration. We found that the population structure of the O. mykiss in these rivers appears to be influenced more by the presence of a barrier to upstream migration than by life history type. The sex ratio of resident O. mykiss located above a barrier, and smolts captured in screw traps was significantly skewed in favor of females, whereas the reverse was true below the barriers, suggesting that male resident O. mykiss readily migrate downstream over the barrier, and that precocious male maturation may be occurring in the anadromous populations. Through paternity analyses, we also provide direct confirmation that resident O. mykiss can produce offspring that become anadromous. Most (89% of the resident O. mykiss that produced anadromous offspring were males. Our results add to the growing body of evidence that shows that gene flow does readily occur between sympatric resident and anadromous O. mykiss life history types, and indicates that resident O. mykiss populations may be a potential repository of genes for the anadromous life history type.

  14. Wall-Resolved Large-Eddy Simulation of Flow Separation Over NASA Wall-Mounted Hump

    Science.gov (United States)

    Uzun, Ali; Malik, Mujeeb R.

    2017-01-01

    This paper reports the findings from a study that applies wall-resolved large-eddy simulation to investigate flow separation over the NASA wall-mounted hump geometry. Despite its conceptually simple flow configuration, this benchmark problem has proven to be a challenging test case for various turbulence simulation methods that have attempted to predict flow separation arising from the adverse pressure gradient on the aft region of the hump. The momentum-thickness Reynolds number of the incoming boundary layer has a value that is near the upper limit achieved by recent direct numerical simulation and large-eddy simulation of incompressible turbulent boundary layers. The high Reynolds number of the problem necessitates a significant number of grid points for wall-resolved calculations. The present simulations show a significant improvement in the separation-bubble length prediction compared to Reynolds-Averaged Navier-Stokes calculations. The current simulations also provide good overall prediction of the skin-friction distribution, including the relaminarization observed over the front portion of the hump due to the strong favorable pressure gradient. We discuss a number of problems that were encountered during the course of this work and present possible solutions. A systematic study regarding the effect of domain span, subgrid-scale model, tunnel back pressure, upstream boundary layer conditions and grid refinement is performed. The predicted separation-bubble length is found to be sensitive to the span of the domain. Despite the large number of grid points used in the simulations, some differences between the predictions and experimental observations still exist (particularly for Reynolds stresses) in the case of the wide-span simulation, suggesting that additional grid resolution may be required.

  15. Numerical Investigation of Flow Separation Control on a Highly Loaded Compressor Cascade by Plasma Aerodynamic Actuation

    Institute of Scientific and Technical Information of China (English)

    ZHAO Xiaohu; LI Yinghong; WU Yun; ZHU Tao; LI Yiwen

    2012-01-01

    To discover the characteristic of separated flows and mechanism of plasma flow control on a highly loaded compressor cascade,numerical investigation is conducted.The simulation method is validated by oil flow visualization and pressure distribution.The loss coefficients,streamline patterns,and topology structure as well as vortex structure are analyzed.Results show thai the numbers of singular points increase and three pairs of additional singular points of topology structure on solid surface generate with the increase of angle of attack,and the total pressure loss increases greatly.There are several principal vortices inside the cascade passage.The pressure side leg of horse-shoe vortex coexists within a specific region together with passage vortex,but finally merges into the latter.Comer vortex exists independently and does not evolve from the suction side leg of horse-shoe vortex.One pair of radial coupling-vortex exists near blade trailing edge and becomes the main part of backflow on the suction surface.Passage vortex interacts with the concentrated shedding vortex and they evolve into a large-scale vortex rotating in the direction opposite to passage vortex.The singular points and separation lines represent the basic separation feature of cascade passage.Plasma actuation has better effect at low freestream velocity,and the relative reductions of pitch-averaged total pressure loss coefficient with different actuation layouts of five and two pairs of electrodes are up to 30.8% and 26.7% while the angle of attack is 2°.Plasma actuation changes the local topology structure,but does not change the number relation of singular points.One pair of additional singular point of topology structure generates with plasma actuation and one more reattachment line appears,both of which break the separation line on the suction surface.

  16. Flow-parametric regulation of shear-driven phase separation in two and three dimensions

    Science.gov (United States)

    Ã` Náraigh, Lennon; Shun, Selma; Naso, Aurore

    2015-06-01

    The Cahn-Hilliard equation with an externally prescribed chaotic shear flow is studied in two and three dimensions. The main goal is to compare and contrast the phase separation in two and three dimensions, using high-resolution numerical simulation as the basis for the study. The model flow is parametrized by its amplitudes (thereby admitting the possibility of anisotropy), length scales, and multiple time scales, and the outcome of the phase separation is investigated as a function of these parameters as well as the dimensionality. In this way, a parameter regime is identified wherein the phase separation and the associated coarsening phenomenon are not only arrested but in fact the concentration variance decays, thereby opening up the possibility of describing the dynamics of the concentration field using the theories of advection diffusion. This parameter regime corresponds to long flow correlation times, large flow amplitudes and small diffusivities. The onset of this hyperdiffusive regime is interpreted by introducing Batchelor length scales. A key result is that in the hyperdiffusive regime, the distribution of concentration (in particular, the frequency of extreme values of concentration) depends strongly on the dimensionality. Anisotropic scenarios are also investigated: for scenarios wherein the variance saturates (corresponding to coarsening arrest), the direction in which the domains align depends on the flow correlation time. Thus, for correlation times comparable to the inverse of the mean shear rate, the domains align in the direction of maximum flow amplitude, while for short correlation times, the domains initially align in the opposite direction. However, at very late times (after the passage of thousands of correlation times), the fate of the domains is the same regardless of correlation time, namely alignment in the direction of maximum flow amplitude. A theoretical model to explain these features is proposed. These features and the theoretical

  17. Evaluation of a new high power, wide separation laser Doppler probe : Potential measurement of deeper tissue blood flow

    NARCIS (Netherlands)

    Clough, Geraldine; Chipperfield, Andrew; Byrne, Christopher; de Mul, Frits; Gush, Rodney

    2009-01-01

    Objective: To compare the output from a novel high power, wide separation laser Doppler flow probe (DP1-V2-HP, 4 mm, with IRLD20) with that of a standard flow probe (DP1-V2, 0.5 mm, with DRT4) (Moor UK) and to explore its potential for use in the noninvasive measurement of blood flow in deeper tissu

  18. Massive separation of turbulent Couette flow in a one-sided expansion channel

    Energy Technology Data Exchange (ETDEWEB)

    El Khoury, George K. [Department of Marine Technology, Norwegian University of Science and Technology, Trondheim NO-7491 (Norway); Andersson, Helge I.; Barri, Mustafa [Department of Energy and Process Engineering, Norwegian University of Science and Technology, Trondheim NO-7491 (Norway); Pettersen, Bjornar, E-mail: bjornar.pettersen@ntnu.n [Department of Marine Technology, Norwegian University of Science and Technology, Trondheim NO-7491 (Norway)

    2010-06-15

    Direct numerical simulation has been performed to study wall-driven flow over a backward-facing step at Reynolds number Re=5200 based on the step height h and the upper-wall velocity U{sub w}. The flow configuration consisted of a step with height equal to that of the upstream channel yielding an expansion ratio 2:1. Instantaneous enstrophy contours revealed the formation of Kelvin-Helmholtz instabilities downstream of the step. Intense velocity and vorticity fluctuations were generated in the shear-layer formed between the bulk flow and the massive recirculation zone in the lee of the step. Extraordinarily high turbulence levels persisted in the center region even 7.5h downstream of the step, i.e. where the separated shear-layer reattached to the wall. A fully redeveloped Couette flow cannot be reached in the downstream part of the channel due to the principle of mass conservation. The local wall pressure coefficient gave evidence of an adverse pressure gradient in the recovery region, where a Couette-Poiseuille flow type prevailed.

  19. Cluster-based control of a separating flow over a smoothly contoured ramp

    Science.gov (United States)

    Kaiser, Eurika; Noack, Bernd R.; Spohn, Andreas; Cattafesta, Louis N.; Morzyński, Marek

    2017-01-01

    The ability to manipulate and control fluid flows is of great importance in many scientific and engineering applications. The proposed closed-loop control framework addresses a key issue of model-based control: The actuation effect often results from slow dynamics of strongly nonlinear interactions which the flow reveals at timescales much longer than the prediction horizon of any model. Hence, we employ a probabilistic approach based on a cluster-based discretization of the Liouville equation for the evolution of the probability distribution. The proposed methodology frames high-dimensional, nonlinear dynamics into low-dimensional, probabilistic, linear dynamics which considerably simplifies the optimal control problem while preserving nonlinear actuation mechanisms. The data-driven approach builds upon a state space discretization using a clustering algorithm which groups kinematically similar flow states into a low number of clusters. The temporal evolution of the probability distribution on this set of clusters is then described by a control-dependent Markov model. This Markov model can be used as predictor for the ergodic probability distribution for a particular control law. This probability distribution approximates the long-term behavior of the original system on which basis the optimal control law is determined. We examine how the approach can be used to improve the open-loop actuation in a separating flow dominated by Kelvin-Helmholtz shedding. For this purpose, the feature space, in which the model is learned, and the admissible control inputs are tailored to strongly oscillatory flows.

  20. Unsteady flow with separation behind a shock wave diffracting over curved walls

    Science.gov (United States)

    Law, C.; Muritala, A. O.; Skews, B. W.

    2014-05-01

    The unsteady separation of the compressible flow field behind a diffracting shock wave was investigated along convex curved walls, using shock tube experimentation at large length and time scales, complemented by numerical computation. Tests were conducted at incident shock Mach numbers of 1.5 and 1.6 over a 100 mm radius wall over a dimensionless time range up to 6.45. The development of the near wall flow at 1.5 has been described in detail and is very similar to that observed for slightly lower 's at 1.6. Computations were performed at wall radii of 100 and 200 mm and for incident shock Mach numbers from 1.5 up to and including Mach 2.0. Comparing dimensionless times for different size walls shows that for a given value of the flow field is very similar for the various wall radii published to date and tested in this study. Previously published results that were examined alongside the results from this study had typical values of . At the later times presented here, flow features were observed that previously had only been observed at higher Mach numbers. The larger length scales allowed for a degree of Reynolds number independence in the results published here. The effect of turbulence on the numerical and experimental results could not be adequately examined due to limitations of the flow imaging system used and a number of questions remain unanswered.

  1. A peculiar segmented flow microfluidics for isoquercitrin biosynthesis based on coupling of reaction and separation.

    Science.gov (United States)

    Gong, An; Gu, Shuang-Shuang; Wang, Jun; Sheng, Sheng; Wu, Fu-An

    2015-10-01

    A segmented flow containing a buffer-ionic liquid/solvent in a micro-channel reactor was applied to synthesize isoquercitrin by the hesperidinase-catalyzed selective hydrolysis of rutin, based on a novel system of reaction coupling with separation. Within the developed microchannel reactor with one T-shaped inlet and outlet, the maximum isoquercitrin yield (101.7 ± 2.6%) was achieved in 20 min at 30 °C and 4 μL/min. Compared with a continuous-flow reactor, reaction rate was increased 4-fold due to a glycine-sodium hydroxide:[Bmim][BF4]/glycerol triacetate (1:1, v/v) system that formed a slug flow in microchannel and significantly increased mass transfer rates. The mass transfer coefficient significantly increased and exhibited a linear relationship with the flow rate. Hesperidinase could be efficiently reused at least 5 times, without losing any activity. The bonding mechanism and secondary structure of hesperidinase indicated that hesperidinase had a greater affinity to rutin at a production rate of 4 μL/min in this segmented flow microreactor.

  2. Separation of a binary mixture of pesticides in fruits using a flow-through optosensor.

    Science.gov (United States)

    Llorent-Martínez, E J; Delgado-Blanca, I; Ruiz-Medina, A; Ortega-Barrales, P

    2013-10-15

    A flow-through optosensor is here proposed for the determination of mixtures of two widely used pesticides, carbendazim and o-phenylphenol, in fruits. The pesticides are separated on-line using an additional amount of solid support, C18 silica gel, in the flow-through cell. The resolution is performed due to the different retention/desorption kinetics of the analytes when interacting with the C18 microbeads. Therefore, both separation and determination are integrated in the same cell, considerably simplifying the system. In addition, the use of Sequential Injection Analysis provides a high degree of automation and minimum wastes generation. After the analytes are separated, their native fluorescence is measured, obtaining linearity in the 2.0-30 and 1.1-20 mg kg(-1) ranges for carbendazim and o-phenylphenol. The detection limits are 0.60 and 0.33 mg kg(-1) for carbendazim and o-phenylphenol respectively. The proposed method fulfills the maximum residue limits (MRLs) established in Europe and USA for these pesticides in cherries, pineapple, and mango: 5-10 mg kg(-1). In order to demonstrate the suitability of the method, several samples have been analyzed and the obtained results compared with a chromatographic method.

  3. Energy and Flow Separation in the Vortex Tube : A Numerical Investigation

    Directory of Open Access Journals (Sweden)

    R. S. Maurya

    2013-10-01

    Full Text Available As a localized cooling device the vortex tube is being used is several applications due to its simplicity, robustness and maintenance free service. Its design still depends on experiment based empirical relations and thumb rules. Capturing complete flow and energy separation features of vortex tube through experimentation is difficult due to the complexity associated to flow Numerical investigation of the vortex tube presented here intends to bring out unexplored features. The work is done on a 3D model of vortex tube with 6 nozzles and an adjustable cone valve. The parameters varied in the investigation are supply air pressure from 2 to 6 bars, the orifice diameter from 5 to 10 mm, L/D ratio from 4 to 20. The mechanism of flow and energy separation is completely explained based on present work. The impact of important performance parameters such as supply pressure, cold orifice diameter, tube diameter and its length suggest improvement in the present thumb rule for better vortex tube performance. Present work investigates the effect of working environment also where tube is assumed work under constant wall temperature and constant wall heat flux condition. It concludes that cooling performance of the tube is independent of thermal condition imposed on its wall.

  4. An improved turbulence model for separation flow in a centrifugal pump

    Directory of Open Access Journals (Sweden)

    Yun Ren

    2016-06-01

    Full Text Available For the stable and reliable operation of centrifugal pump, the transient flow must be studied and the separation region should be avoided. Three-dimensional, incompressible, steady, and transient flows in a centrifugal pump at specific speed within 74 were numerically studied using shear stress transport k-ω turbulence model, and an improved explicit algebraic Reynolds stress model–rotation-curvature turbulence model was proposed by considering the effects of rotation and curvature in the impeller passages in this work. Steady and transient computations were conducted to compare with the experiments. The comparison of pump hydraulic performance showed that the explicit algebraic Reynolds stress model–rotation-curvature turbulence model was better than the original model, especially between 0.6QBEP and 1.2QBEP; the improved model could enhance the head prediction of pump by about 1%–7% than that with the original model. Then, the visualization of the vortex evolution was observed to validate the unsteady simulations. Good agreement was investigated between calculations and visualizations. It is indicated that the explicit algebraic Reynolds stress model–rotation-curvature model can successfully capture the separation flow.

  5. Localisation of flow separation and transition over a pitching NACA0012 airfoil at transitional Reynolds number

    Science.gov (United States)

    Rudmin, Daniel

    Previous research at RMC has cataloged the occurrence of limit cycle oscillations at low-to-moderate Reynolds numbers for an elastically mounted aeroelastic airfoil. These oscillations were attributed to boundary layer separation and the formation of a laminar separation bubble. For this thesis, an instrumented and motor-driven oscillating airfoil rig was designed and fabricated for the purpose of investigating the boundary layer of a NACA-0012 airfoil. The oscillating airfoil was driven by a servo motor to mimic the observed aeroelastic pitching with a sinusoid of matched amplitude and frequency. Hot-wire anemometry was used to investigate the near wake of the new motor-driven airfoil and compare it with the aeroelastic experiment. A chord-wise array of hot-film sensors captured the boundary layer state during the airfoil pitching oscillation. A novel analysis technique is introduced; A sliding window (in time) cross-correlation of adjacent sensors was used to detect dynamic laminar separation. Wind tunnel tests were performed at static angles-of-attack, for quasi-static very low frequency sweeps to verify the technique, and for selected cases of oscillations obtained with the aeroelastic rig. The new detection method was verified against the existing static techniques of phase reversal signature detection and signal cross-correlation by comparing quasi-static and static results. A map of the laminar separation bubble was produced for fixed angles of attack as well as for the pitching airfoil. The presence of a laminar separation was linked to the occurrence and characteristics of the limit cycle oscillations. Keywords: laminar separation, NACA0012, hot-film, hot-wire, anemometry, transitional flow, aeroelasticity.

  6. Location, location, location

    NARCIS (Netherlands)

    Anderson, S.P.; Goeree, J.K.; Ramer, R.

    1997-01-01

    We analyze the canonical location-then-price duopoly game with general log- concave consumer densities. A unique pure-strategy equilibrium to the two-stage game exists if the density is not "too asymmetric" and not "too concave." These criteria are satisfied by many commonly used densities. Equilibr

  7. Design and Development of Gas-Liquid Cylindrical Cyclone Compact Separators for Three-Phase Flow

    Energy Technology Data Exchange (ETDEWEB)

    Mohan, R.S.; Shoham, O.

    2001-01-10

    The objective of this five-year project (October 1997--September 2002) was to expand the current research activities of Tulsa University Separation Technology Projects (TUSTP) to multiphase oil/water/gas separation. This project was executed in two phases. Phase I (1997--2000) focused on the investigations of the complex multiphase hydrodynamic flow behavior in a three-phase Gas-Liquid Cylindrical Cyclone (GLCC) Separator. The activities of this phase included the development of a mechanistic model, a computational fluid dynamics (CFD) simulator, and detailed experimentation on the three-phase GLCC. The experimental and CFD simulation results will be suitably integrated with the mechanistic model. In Phase II (2000--2002), the developed GLCC separator will be tested under high pressure and real crude conditions. This is crucial for validating the GLCC design for field application and facilitating easy and rapid technology deployment. Design criteria for industrial applications will be developed based on these results and will be incorporated into the mechanistic model by TUSTP.

  8. Numerical investigation on flow behavior and energy separation in a micro-scale vortex tube

    Directory of Open Access Journals (Sweden)

    Rahbar Nader

    2015-01-01

    Full Text Available There are a few experimental and numerical studies on the behaviour of micro-scale vortex tubes. The intention of this work is to investigate the energy separation phenomenon in a micro-scale vortex tube by using the computational fluid dynamic. The flow is assumed as steady, turbulent, compressible ideal gas, and the shear-stress transport sst k-w is used for modeling of turbulence phenomenon. The results show that 3-D CFD simulation is more accurate than 2-D axisymmetric one. Moreover, optimum cold-mass ratios to maximize the refrigeration-power and isentropicefficiency are evaluated. The results of static temperature, velocity magnitude and pressure distributions show that the temperature-separation in the micro-scale vortex tube is a function of kinetic-energy variation and air-expansion in the radial direction.

  9. Numerical investigation of the LM MHD flows in a curved duct with an FCI with varying slot locations

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Jong Hoon; Yan, Yue [Department of Mechanical Engineering, Graduate School, Kyung Hee University, Yong-in si, Kyunggi-do 446-701 (Korea, Republic of); Kim, Chang Nyung, E-mail: cnkim@khu.ac.kr [Department of Mechanical Engineering, College of Engineering, Kyung Hee University, Yong-in si, Kyunggi-do 446-701 (Korea, Republic of)

    2016-04-15

    Highlights: • This study numerically investigates the liquid-metal magnetohydrodynamic flows in a curved duct with an FCI. • The effects of the location of FCI slot and of the curvature radius on the flow behavior are reviewed. • The influence of the FCI slot position on the equalization of the pressure in the inner fluid region (inside the FCI) and the gap fluid region (outer the FCI) is examined. - Abstract: This study numerically investigates the liquid-metal (LM) magnetohydrodynamic (MHD) flows in a curved duct with an FCI having three different slot locations and having no slot under a uniform magnetic field perpendicular to the duct. The flow velocity, current density, electric potential, Lorentz force, and pressure in different flow situations are presented in detail. The effects of the location of FCI slot and of the curvature radius on the flow behavior are reviewed. The flow field is examined with an introduction of the electric-field component and electro-motive component of the current, allowing us to analyze the interdependency of the flow variables. The effect of the FCI slot position on the equalization of the pressure in the inner fluid region (inside the FCI) and the gap fluid region (outer the FCI) is examined. The result shows that and the case with an FCI slot located in the neutral position yields the smallest pressure gradient in the main flow direction among the cases with an FCI slot, resulting in the smallest pressure drop. Also, in a flow situation with smaller radius of curvature with the FCI slot in the neutral position, the axial velocity near the inner (in terms of the curvature) part of a cross-section is higher than that near the outer part.

  10. Free flow electrophoresis separation and AMS quantitation of C-naphthalene-protein adducts.

    Science.gov (United States)

    Buchholz, Bruce A; Haack, Kurt W; Sporty, Jennifer L; Buckpitt, Alan R; Morin, Dexter

    2010-04-01

    Naphthalene is a volatile aromatic hydrocarbon to which humans are exposed from a variety of sources including mobile air sources and cigarette smoke. Naphthalene produces dose- (concentration) dependent injury to airway epithelial cells of murine lung which is observed at concentrations well below the current occupational exposure standard. Toxicity is dependent upon the cytochrome P450 mediated metabolic activation of the parent substrate to unstable metabolites which become bound covalently to tissue proteins. Nearly 70 proteins have been identified as forming adducts with reactive naphthalene metabolites using in vitro systems but very little work has been conducted in vivo because reasonably large amounts (100 μCi) of (14)C labeled parent compound must be administered to generate detectable adduct levels on storage phosphor screens following separation of labeled proteins by 2 D gel electrophoresis. The work described here was done to provide proof of concept that protein separation by free flow electrophoresis followed by AMS detection of protein fractions containing protein bound reactive metabolites would provide adducted protein profiles in animals dosed with trace quantities of labeled naphthalene. Mice were administered 200 mg/kg naphthalene intraperitoneally at a calculated specific activity of 2 DPM/nmol (1 pCi/nmol) and respiratory epithelial tissue was obtained by lysis lavage 4 hr post injection. Free flow electrophoresis (FFE) separates proteins in the liquid phase over a large pH range (2.5-11.5) using low molecular weight acids and bases to modify the pH. The apparatus separates fractions into standard 96-well plates that can be used in other protein analysis techniques. The buffers of the fractions have very high carbon content, however, and need to be dialyzed to yield buffers compatible with (14)C-AMS. We describe the processing techniques required to couple FFE to AMS for quantitation of protein adducts.

  11. Control of Separating Flow Behind a Step by Means of Slotted Ribs

    Science.gov (United States)

    D'yachenko, A. Yu.; Zhdanov, V. L.; Smul'skii, Ya. I.; Terekhov, V. I.

    2017-05-01

    One of the promising and simplest methods of passively controlling separating flow behind a backward-facing step with the aid of slotted ribs is considered. The size and number of slots were varied in wide ranges in experiments so that the relative area of the slots amounted to R = Fs/Fr = 0-1. The influence of the longitudinal vortices induced by the teeth in a rib on the distribution of pressure and heat transfer rate in the recirculation region in the case of varying the sizes of slots in a rib, the spacing between them, and the position of the rib relative to the backwardfacing step has been studied. The effectiveness of using slotted ribs for reducing the length and intensity of the region of reverse flow behind the step is shown.

  12. Visualization of flow separation and control by vortex generators on an single flap in landing configuration

    Directory of Open Access Journals (Sweden)

    Matějka Milan

    2012-04-01

    Full Text Available This paper focuses on a suppression of the flow separation, which occurs on a deflected flap, by means of vortex generators (VG's. An airfoil NACA 63A421 with a simple flap and vane-type vortex generators were used. The investigation was carried out by using experimental and numerical methods. The data from the numerical simulation of the flapped airfoil without VG's control were used for the vortex generator design. Two sizes, two different shapes and various spacing of the vortex generators were tested. The flow past the airfoil was visualized through three methods, namely tuft filaments technique, oil and thermo camera visualization. The experiments were performed in closed circuit wind tunnels with closed and open test sections. The lift curves for both cases without and with vortex generators were acquired for a lift coefficient improvement determination. The improvement was achieved for several cases by means all of the applied methods.

  13. Active Flow Separation Control of a Laminar Airfoil at Low Reynolds Number

    Science.gov (United States)

    Packard, Nathan Owen

    Detailed investigation of the NACA 643-618 is obtained at a Reynolds number of 6.4x104 and angle of attack sweep of -5° locked investigation, by way of particle image velocimetry, at ten degrees angle of attack illuminates physical mechanisms responsible for separation control of pulsed actuation at a low frequency and duty cycle. Temporal resolution of large structure formation and wake shedding is obtained, revealing a key mechanism for separation control. The Kelvin-Helmholtz instability is identified as responsible for the formation of smaller structures in the separation region which produce favorable momentum transfer, assisting in further thinning the separation region and then fully attaching the boundary layer. Closed-loop separation control of an oscillating NACA 643-618 airfoil at Re = 6.4x104 is investigated in an effort to autonomously minimize control effort while maximizing aerodynamic performance. High response sensing of unsteady flow with on-surface hot-film sensors placed at zero, twenty, and forty percent chord monitors the airfoil performance and determines the necessity of active flow control. Open-loop characterization identified the use of the forty percent sensor as the actuation trigger. Further, the sensor at twenty percent chord is used to distinguish between pre- and post- leading edge stall; this demarcation enables the utilization of optimal blowing parameters for each circumstance. The range of effectiveness of the employed control algorithm is explored, charting the practicality of the closed-loop control algorithm. To further understand the physical mechanisms inherent in the control process, the transients of the aerodynamic response to flow control are investigated. The on-surface hot-film sensor placed at the leading edge is monitored to understand the time delays and response times associated with the initialization of pulsed normal blowing. The effects of angle of attack and pitch rate on these models are investigated. Black

  14. PIV study on a shock-induced separation in a transonic flow

    Energy Technology Data Exchange (ETDEWEB)

    Sartor, Fulvio; Losfeld, Gilles; Bur, Reynald [ONERA, Meudon (France)

    2012-09-15

    A transonic interaction between a steady shock wave and a turbulent boundary layer in a Mach 1.4 channel flow is experimentally investigated by means of particle image velocimetry (PIV). In the test section, the lower wall is equipped with a contour profile shaped as a bump allowing flow separation. The transonic interaction, characterized by the existence in the outer flow of a lambda shock pattern, causes the separation of the boundary layer, and a low-speed recirculating bubble is observed downstream of the shock foot. Two-component PIV velocity measurements have been performed using an iterative gradient-based cross-correlation algorithm, providing high-speed and flexible calculations, instead of the classic multi-pass processing with FFT-based cross-correlation. The experiments are performed discussing all the hypotheses linked to the experimental set-up and the technique of investigation such as the two-dimensionality assumption of the flow, the particle response assessment, the seeding system, and the PIV correlation uncertainty. Mean velocity fields are presented for the whole interaction with particular attention for the recirculating bubble downstream of the detachment, especially in the mixing layer zone where the effects of the shear stress are most relevant. Turbulence is discussed in details, the results are compared to previous study, and new results are given for the turbulent production term and the return to isotropy mechanism. Finally, using different camera lens, a zoom in the vicinity of the wall presents mean and turbulent velocity fields for the incoming boundary layer. (orig.)

  15. A proposal simulated annealing algorithm for proportional parallel flow shops with separated setup times

    Directory of Open Access Journals (Sweden)

    Helio Yochihiro Fuchigami

    2014-08-01

    Full Text Available This article addresses the problem of minimizing makespan on two parallel flow shops with proportional processing and setup times. The setup times are separated and sequence-independent. The parallel flow shop scheduling problem is a specific case of well-known hybrid flow shop, characterized by a multistage production system with more than one machine working in parallel at each stage. This situation is very common in various kinds of companies like chemical, electronics, automotive, pharmaceutical and food industries. This work aimed to propose six Simulated Annealing algorithms, their perturbation schemes and an algorithm for initial sequence generation. This study can be classified as “applied research” regarding the nature, “exploratory” about the objectives and “experimental” as to procedures, besides the “quantitative” approach. The proposed algorithms were effective regarding the solution and computationally efficient. Results of Analysis of Variance (ANOVA revealed no significant difference between the schemes in terms of makespan. It’s suggested the use of PS4 scheme, which moves a subsequence of jobs, for providing the best percentage of success. It was also found that there is a significant difference between the results of the algorithms for each value of the proportionality factor of the processing and setup times of flow shops.

  16. Stokes flow paths separation and recirculation cells in X-junctions of varying angle

    CERN Document Server

    Cachile, Mario; Gomba, Juan M; Hulin, Jean-Pierre; Auradou, Harold

    2012-01-01

    Fluid and solute transfer in X-junctions between straight channels is shown to depend critically on the junction angle in the Stokes flow regime. Experimentally, water and a water-dye solution are injected at equal flow rates in two facing channels of the junction: Planar Laser Induced fluorescence (PLIF) measurements show that the largest part of each injected fluid "bounces back" preferentially into the outlet channel at the lowest angle to the injection; this is opposite to the inertial case and requires a high curvature of the corresponding streamlines. The proportion of this fluid in the other channel decreases from 50% at 90\\degree to zero at a threshold angle. These counterintuitive features reflect the minimization of energy dissipation for Stokes flows. Finite elements numerical simulations of a 2D Stokes flow of equivalent geometry con rm these results and show that, below the threshold angle 33.8\\degree recirculation cells are present in the center part of the junction and separate the two injected...

  17. Low-dispersion electrokinetic flows for expanded separation channels in microfluidic systems: multiple faceted interfaces.

    Science.gov (United States)

    Fiechtner, Gregory J; Cummings, Eric B

    2004-02-20

    A novel methodology to design on-chip conduction channels is presented for expansion of low-dispersion separation channels. Designs are examined using two-dimensional numerical solutions of the Laplace equation with a Monte Carlo technique to model diffusion. The design technique relies on trigonometric relations that apply for ideal electrokinetic flows. Flows are rotated and stretched along the abrupt interface between adjacent regions having differing specific permeability. Multiple interfaces can be placed in series along a channel. The resulting channels can be expanded to extreme widths while minimizing dispersion of injected analyte bands. These channels can provide a long path length for line-of-sight optical absorption measurements. Expanded sections can be reduced to enable point detection at the exit section of the channel. Designed to be shallow, these channels have extreme aspect ratios in the wide section, greatly increasing the surface-to-volume ratio to increase heat removal and decrease unwanted pressure-driven flow. The use of multiple interfaces is demonstrated by considering several three-interface designs. Faceted flow splitters can be constructed to divide channels into any number of exit channels while minimizing dispersion. The resulting manifolds can be used to construct medians for structural support in wide, shallow channels.

  18. Wall shear stress characterization of a 3D bluff-body separated flow

    Science.gov (United States)

    Fourrié, Grégoire; Keirsbulck, Laurent; Labraga, Larbi

    2013-10-01

    Efficient flow control strategies aimed at reducing the aerodynamic drag of road vehicles require a detailed knowledge of the reference flow. In this work, the flow around the rear slanted window of a generic car model was experimentally studied through wall shear stress measurements using an electrochemical method. The mean and fluctuating wall shear stress within the wall impact regions of the recirculation bubble and the main longitudinal vortex structures which develop above the rear window are presented. Correlations allow a more detailed characterization of the recirculation phenomenon within the separation bubble. In the model symmetry plane the recirculation structure compares well with simpler 2D configurations; specific lengths, flapping motion and shedding of large-scale vortices are observed, these similarities diminish when leaving the middle plane due to the strong three-dimensionality of the flow. A specific attention is paid to the convection processes occurring within the recirculation: a downstream convection velocity is observed, in accordance with 2D recirculations from the literature, and an upstream convection is highlighted along the entire bubble length which has not been underlined in some previous canonical configurations.

  19. Modelling the Timing and Location of Shallow Landslides and Debris Flows Using the SHETRAN Model

    Science.gov (United States)

    Bovolo, C. I.; Bathurst, J. C.

    2006-12-01

    Research on landslide incidence has tended to focus on threshold relationships and has only recently started to look into predicting the location, timing and magnitude of landslide events as a function of climate and geomorphology. In addition, only a few studies consider the post landslide effects of erosion or downstream sediment transport in rivers and most models are limited to areas of a few square kilometres or less. The SHETRAN landslide model is a physically based system which can predictively examine shallow landslide and debris flow incidence on a spatially distributed basis, at the scale of a catchment (New Zealand. A high intensity, single peak rainfall event produced similar number of landslides as a constant low intensity rainfall event of the same duration. Where rainfall increased to a peak at the end of the event, fewer landslides were produced, whilst the least number of landslides were produced where rainfall decreased from a peak at the start of the event. In general, landslides occur at the peak of the rainfall event or at the time of maximum soil saturation but vary according to rainfall distribution and magnitude patterns. Graphs of the number of landslides as a function of rainfall event intensity and duration are given as a series of curves which can be compared with existing thresholds and show the severity of the event in terms of the number of landslides for a given rainfall condition.

  20. Three-Phase AC Optimal Power Flow Based Distribution Locational Marginal Price: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Rui; Zhang, Yingchen

    2017-05-17

    Designing market mechanisms for electricity distribution systems has been a hot topic due to the increased presence of smart loads and distributed energy resources (DERs) in distribution systems. The distribution locational marginal pricing (DLMP) methodology is one of the real-time pricing methods to enable such market mechanisms and provide economic incentives to active market participants. Determining the DLMP is challenging due to high power losses, the voltage volatility, and the phase imbalance in distribution systems. Existing DC Optimal Power Flow (OPF) approaches are unable to model power losses and the reactive power, while single-phase AC OPF methods cannot capture the phase imbalance. To address these challenges, in this paper, a three-phase AC OPF based approach is developed to define and calculate DLMP accurately. The DLMP is modeled as the marginal cost to serve an incremental unit of demand at a specific phase at a certain bus, and is calculated using the Lagrange multipliers in the three-phase AC OPF formulation. Extensive case studies have been conducted to understand the impact of system losses and the phase imbalance on DLMPs as well as the potential benefits of flexible resources.

  1. Kinetic performance limits of constant pressure versus constant flow rate gradient elution separations. Part I: theory.

    Science.gov (United States)

    Broeckhoven, K; Verstraeten, M; Choikhet, K; Dittmann, M; Witt, K; Desmet, G

    2011-02-25

    We report on a general theoretical assessment of the potential kinetic advantages of running LC gradient elution separations in the constant-pressure mode instead of in the customarily used constant-flow rate mode. Analytical calculations as well as numerical simulation results are presented. It is shown that, provided both modes are run with the same volume-based gradient program, the constant-pressure mode can potentially offer an identical separation selectivity (except from some small differences induced by the difference in pressure and viscous heating trajectory), but in a significantly shorter time. For a gradient running between 5 and 95% of organic modifier, the decrease in analysis time can be expected to be of the order of some 20% for both water-methanol and water-acetonitrile gradients, and only weakly depending on the value of V(G)/V₀ (or equivalently t(G)/t₀). Obviously, the gain will be smaller when the start and end composition lie closer to the viscosity maximum of the considered water-organic modifier system. The assumptions underlying the obtained results (no effects of pressure and temperature on the viscosity or retention coefficient) are critically reviewed, and can be inferred to only have a small effect on the general conclusions. It is also shown that, under the adopted assumptions, the kinetic plot theory also holds for operations where the flow rate varies with the time, as is the case for constant-pressure operation. Comparing both operation modes in a kinetic plot representing the maximal peak capacity versus time, it is theoretically predicted here that both modes can be expected to perform equally well in the fully C-term dominated regime (where H varies linearly with the flow rate), while the constant pressure mode is advantageous for all lower flow rates. Near the optimal flow rate, and for linear gradients running from 5 to 95% organic modifier, time gains of the order of some 20% can be expected (or 25-30% when accounting for

  2. Computations of Separated High-Enthalpy Hypersonic Flows: Development of RANS and Variable-Resolution PANS Approaches Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose the development of a high fidelity computational approach for unsteady calculations of strongly separated non-equilibrium high-enthalpy hypersonic flows....

  3. DNS, Enstrophy Balance, and the Dissipation Equation in a Separated Turbulent Channel Flow

    Science.gov (United States)

    Balakumar, Ponnampalam; Rubinstein, Robert; Rumsey, Christopher L.

    2013-01-01

    The turbulent flows through a plane channel and a channel with a constriction (2-D hill) are numerically simulated using DNS and RANS calculations. The Navier-Stokes equations in the DNS are solved using a higher order kinetic energy preserving central schemes and a fifth order accurate upwind biased WENO scheme for the space discretization. RANS calculations are performed using the NASA code CFL3D with the komega SST two-equation model and a full Reynolds stress model. Using DNS, the magnitudes of different terms that appear in the enstrophy equation are evaluated. The results show that the dissipation and the diffusion terms reach large values at the wall. All the vortex stretching terms have similar magnitudes within the buffer region. Beyond that the triple correlation among the vorticity and strain rate fluctuations becomes the important kinematic term in the enstrophy equation. This term is balanced by the viscous dissipation. In the separated flow, the triple correlation term and the viscous dissipation term peak locally and balance each other near the separated shear layer region. These findings concur with the analysis of Tennekes and Lumley, confirming that the energy transfer terms associated with the small-scale dissipation and the fluctuations of the vortex stretching essentially cancel each other, leaving an equation for the dissipation that is governed by the large-scale motion.

  4. Methods for estimating flow-duration curve and low-flow frequency statistics for ungaged locations on small streams in Minnesota

    Science.gov (United States)

    Ziegeweid, Jeffrey R.; Lorenz, David L.; Sanocki, Chris A.; Czuba, Christiana R.

    2015-12-24

    Knowledge of the magnitude and frequency of low flows in streams, which are flows in a stream during prolonged dry weather, is fundamental for water-supply planning and design; waste-load allocation; reservoir storage design; and maintenance of water quality and quantity for irrigation, recreation, and wildlife conservation. This report presents the results of a statewide study for which regional regression equations were developed for estimating 13 flow-duration curve statistics and 10 low-flow frequency statistics at ungaged stream locations in Minnesota. The 13 flow-duration curve statistics estimated by regression equations include the 0.0001, 0.001, 0.02, 0.05, 0.1, 0.25, 0.50, 0.75, 0.9, 0.95, 0.99, 0.999, and 0.9999 exceedance-probability quantiles. The low-flow frequency statistics include annual and seasonal (spring, summer, fall, winter) 7-day mean low flows, seasonal 30-day mean low flows, and summer 122-day mean low flows for a recurrence interval of 10 years. Estimates of the 13 flow-duration curve statistics and the 10 low-flow frequency statistics are provided for 196 U.S. Geological Survey continuous-record streamgages using streamflow data collected through September 30, 2012.

  5. Capillary flow-driven microfluidic device with wettability gradient and sedimentation effects for blood plasma separation

    Science.gov (United States)

    Maria, M. Sneha; Rakesh, P. E.; Chandra, T. S.; Sen, A. K.

    2017-01-01

    We report a capillary flow-driven microfluidic device for blood-plasma separation that comprises a cylindrical well between a pair of bottom and top channels. Exposure of the well to oxygen-plasma creates wettability gradient on its inner surface with its ends hydrophilic and middle portion hydrophobic. Due to capillary action, sample blood self-infuses into bottom channel and rises up the well. Separation of plasma occurs at the hydrophobic patch due to formation of a ‘self-built-in filter’ and sedimentation. Capillary velocity is predicted using a model and validated using experimental data. Sedimentation of RBCs is explained using modified Steinour’s model and correlation between settling velocity and liquid concentration is found. Variation of contact angle on inner surface of the well is characterized and effects of well diameter and height and dilution ratio on plasma separation rate are investigated. With a well of 1.0 mm diameter and 4.0 mm height, 2.0 μl of plasma was obtained (from <10 μl whole blood) in 15 min with a purification efficiency of 99.9%. Detection of glucose was demonstrated with the plasma obtained. Wetting property of channels was maintained by storing in DI water under vacuum and performance of the device was found to be unaffected over three weeks. PMID:28256564

  6. Capillary flow-driven microfluidic device with wettability gradient and sedimentation effects for blood plasma separation

    Science.gov (United States)

    Maria, M. Sneha; Rakesh, P. E.; Chandra, T. S.; Sen, A. K.

    2017-03-01

    We report a capillary flow-driven microfluidic device for blood-plasma separation that comprises a cylindrical well between a pair of bottom and top channels. Exposure of the well to oxygen-plasma creates wettability gradient on its inner surface with its ends hydrophilic and middle portion hydrophobic. Due to capillary action, sample blood self-infuses into bottom channel and rises up the well. Separation of plasma occurs at the hydrophobic patch due to formation of a ‘self-built-in filter’ and sedimentation. Capillary velocity is predicted using a model and validated using experimental data. Sedimentation of RBCs is explained using modified Steinour’s model and correlation between settling velocity and liquid concentration is found. Variation of contact angle on inner surface of the well is characterized and effects of well diameter and height and dilution ratio on plasma separation rate are investigated. With a well of 1.0 mm diameter and 4.0 mm height, 2.0 μl of plasma was obtained (from purification efficiency of 99.9%. Detection of glucose was demonstrated with the plasma obtained. Wetting property of channels was maintained by storing in DI water under vacuum and performance of the device was found to be unaffected over three weeks.

  7. Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples.

    Science.gov (United States)

    Stastna, Miroslava; Slais, Karel

    2010-01-01

    Efficient separation method is a crucial part of the process in which components of highly complex biological sample are identified and characterized. Based on the principles of recently newly established electrophoretic method called divergent flow IEF (DF IEF), we have tested the DF IEF instrument which is able to operate without the use of background carrier ampholytes. We have verified that during separation and focusing of sample consisting of high numbers of proteins (yeast lysate and wheat flour extract), the pH gradient of preparative DF IEF can be created by autofocusing of the sample components themselves without any addition of carrier ampholytes. In DF IEF, the proteins are separated, desalted and concentrated in one step. The fractions of yeast lysate sample, collected at the DF IEF output and subjected to gel IEF, contained the zones of proteins gradually covering the pI values from 3.7 to 8.5. In our experimental arrangement, the highest number of proteins has been found in fractions with pI values around 5.3 as detected by polyacrylamide gel IEF with CBB staining. During DF IEF, the selected protein bands have been concentrated up to 16.8-fold.

  8. Three-dimensional printed magnetophoretic system for the continuous flow separation of avian influenza H5N1 viruses.

    Science.gov (United States)

    Wang, Yuhe; Li, Yanbin; Wang, Ronghui; Wang, Maohua; Lin, Jianhan

    2017-04-01

    As a result of the low concentration of avian influenza viruses in samples for routine screening, the separation and concentration of these viruses are vital for their sensitive detection. We present a novel three-dimensional printed magnetophoretic system for the continuous flow separation of the viruses using aptamer-modified magnetic nanoparticles, a magnetophoretic chip, a magnetic field, and a fluidic controller. The magnetic field was designed based on finite element magnetic simulation and developed using neodymium magnets with a maximum intensity of 0.65 T and a gradient of 32 T/m for dragging the nanoparticle-virus complexes. The magnetophoretic chip was designed by SOLIDWORKS and fabricated by a three-dimensional printer with a magnetophoretic channel for the continuous flow separation of the viruses using phosphate-buffered saline as carrier flow. The fluidic controller was developed using a microcontroller and peristaltic pumps to inject the carrier flow and the viruses. The trajectory of the virus-nanoparticle complexes was simulated using COMSOL for optimization of the carrier flow and the magnetic field, respectively. The results showed that the H5N1 viruses could be captured, separated, and concentrated using the proposed magnetophoretic system with the separation efficiency up to 88% in a continuous flow separation time of 2 min for a sample volume of 200 μL. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. High-Alfa Aerodynamics with Separated Flow Modeled as a Single Nascent Vortex

    Science.gov (United States)

    Antony, Samuel B.; Mukherjee, Rinku

    2017-04-01

    A numerical iterative vortex lattice method is developed to study flow past wing(s) at high angles of attack where the separated flow is modelled using NY nascent vortex filaments. The wing itself is modelled using NX × NY bound vortex rings, where NX and NY are the number of sections along the chord and span of the wing respectively. The strength and position of the nascent vortex along the chord corresponding to the local effective angle of attack are evaluated from the residuals in viscous and potential flow, i.e. (Cl)visc - (Cl)pot and (Cm)visc - (Cm)pot. Hence, the 2D airfoil viscous Cl - α and Cm - α is required as input (from experiment, numerical analysis or CFD). Aerodynamic characteristics and section distribution along span are predicted for 3D wings at a high angle of attack. Effect of initial conditions and existence of multiple solutions in the post-stall region is studied. Results are validated with experiment.

  10. Magnetic separation of particles and cells in ferrofluid flow through a straight microchannel using two offset magnets

    Energy Technology Data Exchange (ETDEWEB)

    Zeng, Jian; Deng, Yanxiang [Department of Mechanical Engineering, Clemson University, Clemson, SC 29634-0921 (United States); Vedantam, Pallavi; Tzeng, Tzuen-Rong [Department of Biological Sciences, Clemson University, Clemson, SC 29634-0314 (United States); Xuan, Xiangchun, E-mail: xcxuan@clemson.edu [Department of Mechanical Engineering, Clemson University, Clemson, SC 29634-0921 (United States)

    2013-11-15

    The separation of particles and cells is critical in many chemical and biological applications. This work presents a simple idea for utilizing a pair of permanent magnets to continuously separate diamagnetic particles and cells in ferrofluid flow through a straight microchannel. The first magnet is placed close to the microchannel for focusing the particle mixture to a single stream without the use of a sheath flow. The second magnet, which is offset from the first magnet and placed farther from the channel, is to displace the aligned particles to dissimilar flow paths for a continuous sorting. This idea is first demonstrated through the separation of 3 μm- and 10 μm-diameter polystyrene particles, where the effects of flow speed and magnet distance are both examined. The experimental data are found to fit well with the predictions of an analytical model. Furthermore, a continuous separation of live yeast cells from 10 μm polystyrene particles is implemented in the same device. - Highlights: • We develop a simple diamagnetic particle and cell separation technique in ferrofluids. • Two offset magnets are used to achieve a sheath-free continuous separation in a straight microchannel. • The technique is demonstrated through the magnetic separation of polystyrene particles and yeast cells. • The effects of ferrofluid speed and magnet-channel distance are examined on particle separation. • The predictions from an analytical model agree with the experimental data quantitatively.

  11. Mechanics of the Separating Surface for a Two-Phase Co-current Flow in a Porous Medium

    DEFF Research Database (Denmark)

    Shapiro, Alexander A.

    2016-01-01

    A mechanical description of an unsteady two-phase co-current flow in a porous medium is developed based on the analysis of the geometry and motion of the surface separating the two phases. It is demonstrated that the flow should be considered as essentially three-dimensional, even if the phase ve...

  12. Domestic wastewater treatment using multi-electrode continuous flow MFCs with a separator electrode assembly design

    KAUST Repository

    Ahn, Yongtae

    2012-10-11

    Treatment of domestic wastewater using microbial fuel cells (MFCs) will require reactors with multiple electrodes, but this presents unique challenges under continuous flow conditions due to large changes in the chemical oxygen demand (COD) concentration within the reactor. Domestic wastewater treatment was examined using a single-chamber MFC (130 mL) with multiple graphite fiber brush anodes wired together and a single air cathode (cathode specific area of 27 m2/m3). In fed-batch operation, where the COD concentration was spatially uniform in the reactor but changed over time, the maximum current density was 148 ± 8 mA/m2 (1,000 Ω), the maximum power density was 120 mW/m2, and the overall COD removal was >90 %. However, in continuous flow operation (8 h hydraulic retention time, HRT), there was a 57 % change in the COD concentration across the reactor (influent versus effluent) and the current density was only 20 ± 13 mA/m2. Two approaches were used to increase performance under continuous flow conditions. First, the anodes were separately wired to the cathode, which increased the current density to 55 ± 15 mA/m2. Second, two MFCs were hydraulically connected in series (each with half the original HRT) to avoid large changes in COD among the anodes in the same reactor. The second approach improved current density to 73 ± 13 mA/m2. These results show that current generation from wastewaters in MFCs with multiple anodes, under continuous flow conditions, can be improved using multiple reactors in series, as this minimizes changes in COD in each reactor. © 2012 Springer-Verlag Berlin Heidelberg.

  13. Phase separation of binary mixtures in shear flow: A numerical study

    Science.gov (United States)

    Corberi; Gonnella; Lamura

    2000-12-01

    The phase-separation kinetics of binary fluids in shear flow is studied numerically in the framework of the continuum convection-diffusion equation based on a Ginzburg-Landau free energy. Simulations are carried out for different temperatures both in d=2 and 3. Our results confirm the qualitative picture put forward by the large-N limit equations studied by Corberi et al. [Phys. Rev. Lett. 81, 3852 (1998)]. In particular, the structure factor is characterized by the presence of four peaks whose relative oscillations give rise to a periodic modulation of the behavior of the rheological indicators and of the average domains sizes. This peculiar pattern of the structure factor corresponds to the presence of domains with two characteristic thicknesses, whose relative abundance changes with time.

  14. On-chip immunomagnetic separation of bacteria by in-flow dynamic manipulation of paramagnetic beads

    Science.gov (United States)

    Ahmed, Shakil; Noh, Jong Wook; Hoyland, James; de Oliveira Hansen, Roana; Erdmann, Helmut; Rubahn, Horst-Günter

    2016-11-01

    Every year, millions of people all over the world fall ill due to the consumption of unsafe food, where consumption of contaminated and spoiled animal origin product is the main cause for diseases due to bacterial growth. This leads to an intense need for efficient methods for detection of food-related bacteria. In this work, we present a method for integration of immunomagnetic separation of bacteria into microfluidic technology by applying an alternating magnetic field, which manipulates the paramagnetic beads into a sinusoidal path across the whole microchannel, increasing the probability for bacteria capture. The optimum channel geometry, flow rate and alternating magnetic field frequency were investigated, resulting in a capture efficiency of 68 %.

  15. Development of an Immunomagnetic Separation Method for Viable Salmonella Typhimurium Detected by Flow Cytometry

    DEFF Research Database (Denmark)

    Ahmed, Shakil; Rubahn, Horst-Günter; Erdmann, Helmut

    2016-01-01

    for detection of food-related bacteria. In this study, a flow cytometry based immunomagnetic separation (IMS) method for the isolation and enrichment of Salmonella Typhimurium from liquid samples was developed and optimized. Both polyclonal and monoclonal antibodies have been used to couple with 1 micron sized......A very small number of bacterial pathogens may have fatal effects on food safety. In spite of having great advancements in bioanalytical methods, most of the accepted detection methods are still cultivation based and thus time consuming. This leads to an intense need for efficient and rapid methods...... and bacteria, immunocapture time, staining and buffering conditions for the viability assays were optimized. The capture efficiency of IMS was>98% for a range of Salmonella Typhimurium cell concentrations from 103 to 105/mL using 108/mL bead concentration. The method proved to have high (98%) specificity...

  16. Recurrence CFD - a novel approach to simulate multiphase flows with strongly separated time scales

    CERN Document Server

    Lichtenegger, Thomas

    2016-01-01

    Classical Computational Fluid Dynamics (CFD) of long-time processes with strongly separated time scales is computationally extremely demanding if not impossible. Consequently, the state-of-the-art description of such systems is not capable of real-time simulations or online process monitoring. In order to bridge this gap, we propose a new method suitable to decouple slow from fast degrees of freedom in many cases. Based on the recurrence statistics of unsteady flow fields, we deduce a recurrence process which enables the generic representation of pseudo-periodic motion at high spatial and temporal resolution. Based on these fields, passive scalars can be traced by recurrence CFD. While a first, Eulerian Model A solves a passive transport equation in a classical implicit finite-volume environment, a second, Lagrangian Model B propagates fluid particles obeying a stochastic differential equation explicitly. Finally, this new concept is tested by two multiphase processes - a lab scale oscillating bubble column a...

  17. Is vent location an important factor in the assessment of pyroclastic flow hazard from sub-Plinian events at Vesuvius?

    Science.gov (United States)

    Marianelli, P.; Esposti Ongaro, T.; Neri, A.; Cavazzoni, C.; Erbacci, G.

    2009-04-01

    Reconstructions of vent location of past sub-Plinian (and Plinian) eruptions of Vesuvius show a significant spatial variability of the vent inside the caldera area. Moreover, it is likely that the exact location of the vent of a future explosive event of that type at Vesuvius will be unknown until the onset of the event itself. Nevertheless, to our knowledge, no studies exist that analyse the influence of vent location on the simulated eruptive scenarios. This omission could be particularly critical for the assessment of pyroclastic flow (or pyroclastic density current, PDC) hazard due to the specific morphology of the Somma-Vesuvius complex. In this work, we present new numerical simulations of column collapse and pyroclastic density current scenarios at Vesuvius by using the transient 3D multiphase flow code PDAC (Esposti Ongaro et al., Parallel Computing, 33, 2007). Simulations have been carried out by assuming three different locations of the vent within the caldera area: the first is in Valle del Gigante, between Mt. Somma ridge and the present Gran Cono, the second is in Piano delle Ginestre, to the west of the Gran Cono, while the third one is in Valle dell'Inferno, in the south-eastern direction with respect to the Gran Cono. Source conditions for all cases refer to a sub-Plinian event and to both partial and nearly-total collapse of the eruptive column. Simulation results clearly show the first-order effect of vent location on the propagation directions of PDCs and the areal distribution of pyroclasts, owing to the complex interaction of the flow with the proximal volcano morphology. Comparisons with simulations assuming a central vent located in the position of Gran Cono clearly show the different roles of Mt. Somma, Gran Cono, along with mean slope and channelling effects as a function of the assumed vent location. Estimates of the areas invaded by the flows are presented, although the definition of their runout is particularly difficult due to the

  18. Nanoporous separator and low fuel concentration to minimize crossover in direct methanol laminar flow fuel cells

    Energy Technology Data Exchange (ETDEWEB)

    Hollinger, A.S. [Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 Green Street, Urbana, IL 61801 (United States); Maloney, R.J.; Jayashree, R.S. [Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Matthews Avenue, Urbana, IL 61801 (United States); Natarajan, D.; Markoski, L.J. [INI Power Systems, 175 Southport Drive, Suite 100, Morrisville, NC 27560 (United States); Kenis, P.J.A. [Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 Green Street, Urbana, IL 61801 (United States); Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Matthews Avenue, Urbana, IL 61801 (United States)

    2010-06-01

    Laminar flow fuel cells (LFFCs) overcome some key issues - most notably fuel crossover and water management - that typically hamper conventional polymer electrolyte-based fuel cells. Here we report two methods to further minimize fuel crossover in LFFCs: (i) reducing the cross-sectional area between the fuel and electrolyte streams, and (ii) reducing the driving force of fuel crossover, i.e. the fuel concentration gradient. First, we integrated a nanoporous tracketch separator at the interface of the fuel and electrolyte streams in a single-channel LFFC to dramatically reduce the cross-sectional area across which methanol can diffuse. Maximum power densities of 48 and 70 mW cm{sup -2} were obtained without and with a separator, respectively, when using 1 M methanol. This simple design improvement reduces losses at the cathode leading to better performance and enables thinner cells, which is attractive in portable applications. Second, we demonstrated a multichannel cell that utilizes low methanol concentrations (<300 mM) to reduce the driving force for methanol diffusion to the cathode. Using 125 mM methanol as the fuel, a maximum power density of 90 mW cm{sup -2} was obtained. This multichannel cell further simplifies the LFFC design (one stream only) and its operation, thereby extending its potential for commercial application. (author)

  19. Effects of Particles Collision on Separating Gas–Particle Two-Phase Turbulent Flows

    KAUST Repository

    Sihao, L. V.

    2013-10-10

    A second-order moment two-phase turbulence model incorporating a particle temperature model based on the kinetic theory of granular flow is applied to investigate the effects of particles collision on separating gas–particle two-phase turbulent flows. In this model, the anisotropy of gas and solid phase two-phase Reynolds stresses and their correlation of velocity fluctuation are fully considered using a presented Reynolds stress model and the transport equation of two-phase stress correlation. Experimental measurements (Xu and Zhou in ASME-FED Summer Meeting, San Francisco, Paper FEDSM99-7909, 1999) are used to validate this model, source codes and prediction results. It showed that the particles collision leads to decrease in the intensity of gas and particle vortices and takes a larger effect on particle turbulent fluctuations. The time-averaged velocity, the fluctuation velocity of gas and particle phase considering particles colli-sion are in good agreement with experimental measurements. Particle kinetic energy is always smaller than gas phase due to energy dissipation from particle collision. Moreover, axial– axial and radial–radial fluctuation velocity correlations have stronger anisotropic behaviors. © King Fahd University of Petroleum and Minerals 2013

  20. USM3D Simulations of Saturn V Plume Induced Flow Separation

    Science.gov (United States)

    Deere, Karen; Elmlilgui, Alaa; Abdol-Hamid, K. S.

    2011-01-01

    The NASA Constellation Program included the Ares V heavy lift cargo vehicle. During the design stage, engineers questioned if the Plume Induced Flow Separation (PIFS) that occurred along Saturn V rocket during moon missions at some flight conditions, would also plague the newly proposed rocket. Computational fluid dynamics (CFD) was offered as a tool for initiating the investigation of PIFS along the Ares V rocket. However, CFD best practice guidelines were not available for such an investigation. In an effort to establish a CFD process and define guidelines for Ares V powered simulations, the Saturn V vehicle was used because PIFS flight data existed. The ideal gas, computational flow solver USM3D was evaluated for its viability in computing PIFS along the Saturn V vehicle with F-1 engines firing. Solutions were computed at supersonic freestream conditions, zero degree angle of attack, zero degree sideslip, and at flight Reynolds numbers. The effects of solution sensitivity to grid refinement, turbulence models, and the engine boundary conditions on the predicted PIFS distance along the Saturn V were discussed and compared to flight data from the Apollo 11 mission AS-506.

  1. CFD Simulations of the IHF Arc-Jet Flow: Compression-Pad/Separation Bolt Wedge Tests

    Science.gov (United States)

    Gokcen, Tahir; Skokova, Kristina A.

    2017-01-01

    This paper reports computational analyses in support of two wedge tests in a high enthalpy arc-jet facility at NASA Ames Research Center. These tests were conducted using two different wedge models, each placed in a free jet downstream of a corresponding different conical nozzle in the Ames 60-MW Interaction Heating Facility. Panel test articles included a metallic separation bolt imbedded in the compression-pad and heat shield materials, resulting in a circular protuberance over a flat plate. As part of the test calibration runs, surface pressure and heat flux measurements on water-cooled calibration plates integrated with the wedge models were also obtained. Surface heating distributions on the test articles as well as arc-jet test environment parameters for each test configuration are obtained through computational fluid dynamics simulations, consistent with the facility and calibration measurements. The present analysis comprises simulations of the non-equilibrium flow field in the facility nozzle, test box, and flow field over test articles, and comparisons with the measured calibration data.

  2. Comparative assessment of SAS and DES turbulence modeling for massively separated flows

    Science.gov (United States)

    Zheng, Weilin; Yan, Chao; Liu, Hongkang; Luo, Dahai

    2016-02-01

    Numerical studies of the flow past a circular cylinder at Reynolds number 1.4× 105 and NACA0021 airfoil at the angle of attack 60° have been carried out by scale-adaptive simulation (SAS) and detached eddy simulation (DES), in comparison with the existing experimental data. The new version of the model developed by Egorov and Menter is assessed, and advantages and disadvantages of the SAS simulation are analyzed in detail to provide guidance for industrial application in the future. Moreover, the mechanism of the scale-adaptive characteristics in separated regions is discussed, which is obscure in previous analyses. It is concluded that: the mean flow properties satisfactorily agree with the experimental results for the SAS simulation, although the prediction of the second order turbulent statistics in the near wake region is just reasonable. The SAS model can produce a larger magnitude of the turbulent kinetic energy in the recirculation bubble, and, consequently, a smaller recirculation region and a more rapid recovery of the mean velocity outside the recirculation region than the DES approach with the same grid resolution. The vortex shedding is slightly less irregular with the SAS model than with the DES approach, probably due to the higher dissipation of the SAS simulation under the condition of the coarse mesh.

  3. Polyvinyl Chloride/Silica Nanoporous Composite Separator for All-Vanadium Redox Flow Battery Applications

    Energy Technology Data Exchange (ETDEWEB)

    Wei, Xiaoliang; Nie, Zimin; Luo, Qingtao; Li, Bin; Sprenkle, Vincent L.; Wang, Wei

    2013-04-22

    Redox flow batteries (RFBs) are capable of reversible conversion between electricity and chemical energy. Potential RFB applications resolve around mitigating the discrepancy between electricity production and consumption to improve the stability and utilization of the power infrastructure and tackling the intermittency of renewables such as photovoltaics or wind turbines to enable their reliable integration [1, 2]. Because the energy is stored in externally contained liquid electrolytes and the energy conversion reactions take place at the electrodes, RFBs hold a unique capability to separate energy and power and thus possess considerable design flexibility to meet either energy management driven or power rating oriented grid applications, which is considered to be a unparalleled advantage over conventional solid-state secondary batteries [3]. Other advantages of RFBs include fast response to load changes, high round-trip efficiency, long calender and cycle lives, safe operations, tolerance to deep discharge, etc. [4]. Among various flow battery chemistries, all-vanadium redox flow battery (VRB) was invented by Maria Skyllas-Kazacos at the University of New South Wales in the 1980s [5, 6] and have attracted substantial attention in both research and industrial communities today [7, 8]. A well-recognized advantage that makes VRB stands out among other redox chemistries is the reduced crossover contamination ascribed to employing four different oxidation states of the same vanadium element as the two redox couples. Recently, great progress has led to remarkably improved energy density of VRB by using sulfuric-chloric mixed acid supporting electrolytes that were stable at 2.5M vanadium and had wider operational temperature window of -5~50oC [9], compared with the traditional sulfuric acid VRB system [10].

  4. Analysis of the separated boundary layer flow on the surface and in the wake of blunt trailing edge airfoils

    Science.gov (United States)

    Goradia, S. H.; Mehta, J. M.; Shrewsbury, G. S.

    1977-01-01

    The viscous flow phenomena associated with sharp and blunt trailing edge airfoils were investigated. Experimental measurements were obtained for a 17 percent thick, high performance GAW-1 airfoil. Experimental measurements consist of velocity and static pressure profiles which were obtained by the use of forward and reverse total pressure probes and disc type static pressure probes over the surface and in the wake of sharp and blunt trailing edge airfoils. Measurements of the upper surface boundary layer were obtained in both the attached and separated flow regions. In addition, static pressure data were acquired, and skin friction on the airfoil upper surface was measured with a specially constructed device. Comparison of the viscous flow data with data previously obtained elsewhere indicates reasonable agreement in the attached flow region. In the separated flow region, considerable differences exist between these two sets of measurements.

  5. Conducting Privacy-Preserving Multivariable Propensity Score Analysis When Patient Covariate Information Is Stored in Separate Locations.

    Science.gov (United States)

    Bohn, Justin; Eddings, Wesley; Schneeweiss, Sebastian

    2017-03-15

    Distributed networks of health-care data sources are increasingly being utilized to conduct pharmacoepidemiologic database studies. Such networks may contain data that are not physically pooled but instead are distributed horizontally (separate patients within each data source) or vertically (separate measures within each data source) in order to preserve patient privacy. While multivariable methods for the analysis of horizontally distributed data are frequently employed, few practical approaches have been put forth to deal with vertically distributed health-care databases. In this paper, we propose 2 propensity score-based approaches to vertically distributed data analysis and test their performance using 5 example studies. We found that these approaches produced point estimates close to what could be achieved without partitioning. We further found a performance benefit (i.e., lower mean squared error) for sequentially passing a propensity score through each data domain (called the "sequential approach") as compared with fitting separate domain-specific propensity scores (called the "parallel approach"). These results were validated in a small simulation study. This proof-of-concept study suggests a new multivariable analysis approach to vertically distributed health-care databases that is practical, preserves patient privacy, and warrants further investigation for use in clinical research applications that rely on health-care databases. © The Author 2017. Published by Oxford University Press on behalf of the Johns Hopkins Bloomberg School of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  6. Can a stepwise steady flow computational fluid dynamics model reproduce unsteady particulate matter separation for common unit operations?

    Science.gov (United States)

    Pathapati, Subbu-Srikanth; Sansalone, John J

    2011-07-01

    Computational fluid dynamics (CFD) is emerging as a model for resolving the fate of particulate matter (PM) by unit operations subject to rainfall-runoff loadings. However, compared to steady flow CFD models, there are greater computational requirements for unsteady hydrodynamics and PM loading models. Therefore this study examines if integrating a stepwise steady flow CFD model can reproduce PM separation by common unit operations loaded by unsteady flow and PM loadings, thereby reducing computational effort. Utilizing monitored unit operation data from unsteady events as a metric, this study compares the two CFD modeling approaches for a hydrodynamic separator (HS), a primary clarifier (PC) tank, and a volumetric clarifying filtration system (VCF). Results indicate that while unsteady CFD models reproduce PM separation of each unit operation, stepwise steady CFD models result in significant deviation for HS and PC models as compared to monitored data; overestimating the physical size requirements of each unit required to reproduce monitored PM separation results. In contrast, the stepwise steady flow approach reproduces PM separation by the VCF, a combined gravitational sedimentation and media filtration unit operation that provides attenuation of turbulent energy and flow velocity.

  7. Methods for estimating selected low-flow frequency statistics and mean annual flow for ungaged locations on streams in North Georgia

    Science.gov (United States)

    Gotvald, Anthony J.

    2017-01-13

    The U.S. Geological Survey, in cooperation with the Georgia Department of Natural Resources, Environmental Protection Division, developed regional regression equations for estimating selected low-flow frequency and mean annual flow statistics for ungaged streams in north Georgia that are not substantially affected by regulation, diversions, or urbanization. Selected low-flow frequency statistics and basin characteristics for 56 streamgage locations within north Georgia and 75 miles beyond the State’s borders in Alabama, Tennessee, North Carolina, and South Carolina were combined to form the final dataset used in the regional regression analysis. Because some of the streamgages in the study recorded zero flow, the final regression equations were developed using weighted left-censored regression analysis to analyze the flow data in an unbiased manner, with weights based on the number of years of record. The set of equations includes the annual minimum 1- and 7-day average streamflow with the 10-year recurrence interval (referred to as 1Q10 and 7Q10), monthly 7Q10, and mean annual flow. The final regional regression equations are functions of drainage area, mean annual precipitation, and relief ratio for the selected low-flow frequency statistics and drainage area and mean annual precipitation for mean annual flow. The average standard error of estimate was 13.7 percent for the mean annual flow regression equation and ranged from 26.1 to 91.6 percent for the selected low-flow frequency equations.The equations, which are based on data from streams with little to no flow alterations, can be used to provide estimates of the natural flows for selected ungaged stream locations in the area of Georgia north of the Fall Line. The regression equations are not to be used to estimate flows for streams that have been altered by the effects of major dams, surface-water withdrawals, groundwater withdrawals (pumping wells), diversions, or wastewater discharges. The regression

  8. Numerical study of the phase separation in binary lipid membrane containing protein inclusions under stationary shear flow.

    Science.gov (United States)

    Chen, Xiao-Bo; Shi, Hui-Ji; Niu, Li-Sha

    2009-03-26

    The phase separation of lipids is believed to be responsible for the formation of lipid rafts in biological cell membrane. In the present work, a continuum model and a particle model are constructed to study the phase separation in binary lipid membrane containing inclusions under stationary shear flow. In each model, employing the cell dynamical system (CDS) approach, the kinetic equations of the confusion-advection process are numerically solved. Snapshot figures of the phase morphology are performed to intuitively display such phase evolving process. Considering the effects from both the inclusions and the shear flow, the time growth law of the characteristic domain size is discussed.

  9. Numerical study of the effect of an embedded surface-heat source on the separation bubble of supersonic flow

    Science.gov (United States)

    Degani, D.

    1983-01-01

    A numerical study of the conjugated problem of a separated supersonic flow field and a conductive solid wall with an embedded heat source is presented. Implicit finite-difference schemes were used to solve the two-dimensional time-dependent compressible Navier-Stokes equations and the time-dependent heat-conduction equation for the solid in both general coordinate systems. A detailed comparison between the thin-layer and Navier-Stokes models was made for steady and unsteady supersonic flow and showed insignificant differences. Steady-state and transient cases were computed and the results show that a temperature pulse at the solid-fluid interface can be used to detect the flow direction near the wall in the vicinity of separation without significant distortion of the flow field.

  10. Geophysical flows under location uncertainty, Part I Random transport and general models

    CERN Document Server

    Resseguier, Valentin; Chapron, Bertrand

    2016-01-01

    A stochastic flow representation is considered with the Eulerian velocity decomposed between a smooth large scale component and a rough small-scale turbulent component. The latter is specified as a random field uncorrelated in time. Subsequently, the material derivative is modified and leads to a stochastic version of the material derivative to include a drift correction, an inhomogeneous and anisotropic diffusion, and a multiplicative noise. As derived, this stochastic transport exhibits a remarkable energy conservation property for any realizations. As demonstrated, this pivotal operator further provides elegant means to derive stochastic formulations of classical representations of geophysical flow dynamics.

  11. Optimum reduction of the dynamo threshold by a ferromagnetic layer located in the flow.

    Science.gov (United States)

    Herault, J; Pétrélis, F

    2014-09-01

    We consider a fluid dynamo model generated by the flow on both sides of a moving layer. The magnetic permeability of the layer is larger than that of the flow. We show that there exists an optimum value of magnetic permeability for which the critical magnetic Reynolds number for dynamo onset is smaller than for a nonmagnetic material and also smaller than for a layer of infinite magnetic permeability. We present a mechanism that provides an explanation for recent experimental results. A similar effect occurs when the electrical conductivity of the layer is large.

  12. Modeling, fabrication and plasma actuator coupling of flexible pressure sensors for flow separation detection and control in aeronautical applications

    Science.gov (United States)

    Francioso, L.; De Pascali, C.; Pescini, E.; De Giorgi, M. G.; Siciliano, P.

    2016-06-01

    Preventing the flow separation could enhance the performance of propulsion systems and future civil aircraft. To this end, a fast detection of boundary layer separation is mandatory for a sustainable and successful application of active flow control devices, such as plasma actuators. The present work reports on the design, fabrication and functional tests of low-cost capacitive pressure sensors coupled with dielectric barrier discharge (DBD) plasma actuators to detect and then control flow separation. Finite element method (FEM) simulations were used to obtain information on the deflection and the stress distribution in different-shaped floating membranes. The sensor sensitivity as a function of the pressure load was also calculated by experimental tests. The results of the calibration of different capacitive pressure sensors are reported in this work, together with functional tests in a wind tunnel equipped with a curved wall plate on which a DBD plasma actuator was mounted to control the flow separation. The flow behavior was experimentally investigated by particle image velocimetry (PIV) measurements. Statistical and spectral analysis, applied to the output signals of the pressure sensor placed downstream of the profile leading edge, demonstrated that the sensor is able to discriminate different ionic wind velocity and turbulence conditions. The sensor sensitivity in the 0-100 Pa range was experimentally measured and it ranged between 0.0030 and 0.0046 pF Pa-1 for the best devices.

  13. Improvement of system security with unified-power-flow controller at suitable locations under network contingencies of interconnected systems

    OpenAIRE

    Thukaram, D; Jenkins, L.; Visakha, K

    2005-01-01

    The operation and planning of large interconnected power systems are becoming increasingly complex. To maintain security of such systems, it is desirable to estimate the effect of contingencies and plan suitable measures to improve system security/stability. The paper presents an approach for selection of unified-power-flow-controller (UPFC-) suitable locations considering normal and network contingencies after evaluating the degree of severity of the contingencies. The ranking is evaluated u...

  14. Geophysical flows under location uncertainty, Part II: Quasi-geostrophy and efficient ensemble spreading

    CERN Document Server

    Resseguier, Valentin; Chapron, Bertrand

    2016-01-01

    Models under location uncertainty are derived assuming that a component of the velocity is uncorrelated in time. The material derivative is accordingly modified to include an advection correction, inhomogeneous and anisotropic diffusion terms and a multiplicative noise contribution. In this paper, simplified geophysical dynamics are derived from a Boussinesq model under location uncertainty. Invoking usual scaling approximations and a moderate influence of the subgrid terms, stochastic formulations are obtained for the stratified Quasi-Geostrophy (QG) and the Surface Quasi-Geostrophy (SQG) models. Based on numerical simulations, benefits of the proposed stochastic formalism are demonstrated. A single realization of models under location uncertainty can restore small-scale structures. An ensemble of realizations further helps to assess model error prediction and outperforms perturbed deterministic models by one order of magnitude. Such a high uncertainty quantification skill is of primary interests for assimil...

  15. Nanoporous Polytetrafluoroethylene/Silica Composite Separator as a High-Performance All-Vanadium Redox Flow Battery Membrane

    Energy Technology Data Exchange (ETDEWEB)

    Wei, Xiaoliang; Nie, Zimin; Luo, Qingtao; Li, Bin; Chen, Baowei; Simmons, Kevin L.; Sprenkle, Vincent L.; Wang, Wei

    2013-09-02

    Driven by the motivation of searching for low-cost membrane alternatives, a novel nanoporous polytetrafluoroethylene/silica composite separator has been prepared and evaluated for its use in all-vanadium mixed-acid redox flow battery. This separator consisting of silica particles enmeshed in a polytetrafluoroethylene fibril matrix has no ion exchange capacity and is featured with unique nanoporous structures, which function as the ion transport channels in redox flow battery operation, with an average pore size of 38nm and a porosity of 48%. This separator has produced excellent electrochemical performance in the all-vanadium mixed-acid system with energy efficiency delivery comparable to Nafion membrane and superior rate capability and temperature tolerance. The separator also demonstrates an exceptional capacity retention capability over extended cycling, offering additional operational latitude towards conveniently mitigating the capacity decay that is inevitable for Nafion. Because of the inexpensive raw materials and simple preparation protocol, the separator is particularly low-cost, estimated to be at least an order of magnitude more inexpensive than Nafion. Plus the proven chemical stability due to the same backbone material as Nafion, this separator possesses a good combination of critical membrane requirements and shows great potential to promote market penetration of the all-vanadium redox flow battery by enabling significant reduction of capital and cycle costs.

  16. A study of optimum cowl shapes and flow port locations for minimum drag with effective engine cooling, volume 2

    Science.gov (United States)

    Fox, S. R.; Smetana, F. O.

    1980-01-01

    The listings, user's instructions, sample inputs, and sample outputs of two computer programs which are especially useful in obtaining an approximate solution of the viscous flow over an arbitrary nonlifting three dimensional body are provided. The first program performs a potential flow solution by a well known panel method and readjusts this initial solution to account for the effects of the boundary layer displacement thickness, a nonuniform but unidirectional onset flow field, and the presence of air intakes and exhausts. The second program is effectually a geometry package which allows the user to change or refine the shape of a body to satisfy particular needs without a significant amount of human intervention. An effort to reduce the cruise drag of light aircraft through an analytical study of the contributions to the drag arising from the engine cowl shape and the foward fuselage area and also that resulting from the cooling air mass flowing through intake and exhaust sites on the nacelle is presented. The programs may be effectively used to determine the appropriate body modifications or flow port locations to reduce the cruise drag as well as to provide sufficient air flow for cooling the engine.

  17. A Hybrid Vortex Sheet / Point Vortex Model for Unsteady Separated Flows

    Science.gov (United States)

    Darakananda, Darwin; Eldredge, Jeff D.; Colonius, Tim; Williams, David R.

    2015-11-01

    The control of separated flow over an airfoil is essential for obtaining lift enhancement, drag reduction, and the overall ability to perform high agility maneuvers. In order to develop reliable flight control systems capable of realizing agile maneuvers, we need a low-order aerodynamics model that can accurately predict the force response of an airfoil to arbitrary disturbances and/or actuation. In the present work, we integrate vortex sheets and variable strength point vortices into a method that is able to capture the formation of coherent vortex structures while remaining computationally tractable for control purposes. The role of the vortex sheet is limited to tracking the dynamics of the shear layer immediately behind the airfoil. When parts of the sheet develop into large scale structures, those sections are replaced by variable strength point vortices. We prevent the vortex sheets from growing indefinitely by truncating the tips of the sheets and transfering their circulation into nearby point vortices whenever the length of sheet exceeds a threshold. We demonstrate the model on a variety of canonical problems, including pitch-up and impulse translation of an airfoil at various angles of attack. Support by the U.S. Air Force Office of Scientific Research (FA9550-14-1-0328) with program manager Dr. Douglas Smith is gratefully acknowledged.

  18. DNS/LES Simulations of Separated Flows at High Reynolds Numbers

    Science.gov (United States)

    Balakumar, P.

    2015-01-01

    Direct numerical simulations (DNS) and large-eddy simulations (LES) simulations of flow through a periodic channel with a constriction are performed using the dynamic Smagorinsky model at two Reynolds numbers of 2800 and 10595. The LES equations are solved using higher order compact schemes. DNS are performed for the lower Reynolds number case using a fine grid and the data are used to validate the LES results obtained with a coarse and a medium size grid. LES simulations are also performed for the higher Reynolds number case using a coarse and a medium size grid. The results are compared with an existing reference data set. The DNS and LES results agreed well with the reference data. Reynolds stresses, sub-grid eddy viscosity, and the budgets for the turbulent kinetic energy are also presented. It is found that the turbulent fluctuations in the normal and spanwise directions have the same magnitude. The turbulent kinetic energy budget shows that the production peaks near the separation point region and the production to dissipation ratio is very high on the order of five in this region. It is also observed that the production is balanced by the advection, diffusion, and dissipation in the shear layer region. The dominant term is the turbulent diffusion that is about two times the molecular dissipation.

  19. Experimental study of flow separation control on a low- Re airfoil using leading-edge protuberance method

    Science.gov (United States)

    Zhang, M. M.; Wang, G. F.; Xu, J. Z.

    2014-04-01

    An experimental study of flow separation control on a low- Re c airfoil was presently investigated using a newly developed leading-edge protuberance method, motivated by the improvement in the hydrodynamics of the giant humpback whale through its pectoral flippers. Deploying this method, the control effectiveness of the airfoil aerodynamics was fully evaluated using a three-component force balance, leading to an effectively impaired stall phenomenon and great improvement in the performances within the wide post-stall angle range (22°-80°). To understand the flow physics behind, the vorticity field, velocity field and boundary layer flow field over the airfoil suction side were examined using a particle image velocimetry and an oil-flow surface visualization system. It was found that the leading-edge protuberance method, more like low-profile vortex generator, effectively modified the flow pattern of the airfoil boundary layer through the chordwise and spanwise evolutions of the interacting streamwise vortices generated by protuberances, where the separation of the turbulent boundary layer dominated within the stall region and the rather strong attachment of the laminar boundary layer still existed within the post-stall region. The characteristics to manipulate the flow separation mode of the original airfoil indicated the possibility to further optimize the control performance by reasonably designing the layout of the protuberances.

  20. Using radon to understand parafluvial flows and the changing locations of groundwater inflows in the Avon River, southeast Australia

    Science.gov (United States)

    Cartwright, Ian; Hofmann, Harald

    2016-09-01

    Understanding the location and magnitude of groundwater inflows to rivers is important for the protection of riverine ecosystems and the management of connected groundwater and surface water systems. This study utilizes 222Rn activities and Cl concentrations in the Avon River, southeast Australia, to determine the distribution of groundwater inflows and to understand the importance of parafluvial flow on the 222Rn budget. The distribution of 222Rn activities and Cl concentrations implies that the Avon River contains alternating gaining and losing reaches. The location of groundwater inflows changed as a result of major floods in 2011-2013 that caused significant movement of the floodplain sediments. The floodplain of the Avon River comprises unconsolidated coarse-grained sediments with numerous point bars and sediment banks through which significant parafluvial flow is likely. The 222Rn activities in the Avon River, which are locally up to 3690 Bq m-3, result from a combination of groundwater inflows and the input of water from the parafluvial zone that has high 222Rn activities due to 222Rn emanation from the alluvial sediments. If the high 222Rn activities were ascribed solely to groundwater inflows, the calculated net groundwater inflows would exceed the measured increase in streamflow along the river by up to 490 % at low streamflows. Uncertainties in the 222Rn activities of groundwater, the gas transfer coefficient, and the degree of hyporheic exchange cannot explain a discrepancy of this magnitude. The proposed model of parafluvial flow envisages that water enters the alluvial sediments in reaches where the river is losing and subsequently re-enters the river in the gaining reaches with flow paths of tens to hundreds of metres. Parafluvial flow is likely to be important in rivers with coarse-grained alluvial sediments on their floodplains and failure to quantify the input of 222Rn from parafluvial flow will result in overestimating groundwater inflows to

  1. Prediction of separation flows around a 6:1 prolate spheroid using RANS/LES hybrid approaches

    Institute of Scientific and Technical Information of China (English)

    Zhixiang Xiao; Yufei Zhang; Jingbo Huang; Haixin Chen; Song Fu

    2007-01-01

    This paper presents hybrid Reynolds-averaged Navier-Stokes (RANS) and large-eddy-simulation (LES) methods for the separated flows at high angles of attack around a 6:1 prolate spheroid. The RANS/LES hybrid meth-ods studied in this work include the detached eddy simula-tion (DES) based on Spalart-Allmaras (S-A), Menter's k-w shear-stress-transport (SST) and k-ω with weakly nonlinear eddy viscosity formulation (Wilcox-Durbin+, WD+) mod-els and the zonaI-RANS/LES methods based on the SST and WD+ models. The switch from RANS near the wall to LES in the core flow region is smooth through the implementation of a flow-dependent blending function for the zonal hybrid method. All the hybrid methods are designed to have a RANS mode for the attached flows and have a LES behavior for the separated flows. The main objective of this paper is to apply the hybrid methods for the high Reynolds number separated flows around prolate spheroid at high-incidences.A fourth-order central scheme with fourth-order artificial viscosity is applied for spatial differencing. The fully implicit lower-upper symmetric-Gauss-Seidel with pseudo time sub-iteration is taken as the temporal differentiation. Com-parisons with available measurements are carried out for pressure distribution, skin friction, and profiles of velocity,etc. Reasonable agreement with the experiments, accounting for the effect on grids and fundamental turbulence models,is obtained for the separation flows.

  2. INTRA-URBAN MOVEMENT FLOW ESTIMATION USING LOCATION BASED SOCIAL NETWORKING DATA

    Directory of Open Access Journals (Sweden)

    A. Kheiri

    2015-12-01

    Full Text Available In recent years, there has been a rapid growth of location-based social networking services, such as Foursquare and Facebook, which have attracted an increasing number of users and greatly enriched their urban experience. Location-based social network data, as a new travel demand data source, seems to be an alternative or complement to survey data in the study of mobility behavior and activity analysis because of its relatively high access and low cost. In this paper, three OD estimation models have been utilized in order to investigate their relative performance when using Location-Based Social Networking (LBSN data. For this, the Foursquare LBSN data was used to analyze the intra-urban movement behavioral patterns for the study area, Manhattan, the most densely populated of the five boroughs of New York city. The outputs of models are evaluated using real observations based on different criterions including distance distribution, destination travel constraints. The results demonstrate the promising potential of using LBSN data for urban travel demand analysis and monitoring.

  3. Intra-Urban Movement Flow Estimation Using Location Based Social Networking Data

    Science.gov (United States)

    Kheiri, A.; Karimipour, F.; Forghani, M.

    2015-12-01

    In recent years, there has been a rapid growth of location-based social networking services, such as Foursquare and Facebook, which have attracted an increasing number of users and greatly enriched their urban experience. Location-based social network data, as a new travel demand data source, seems to be an alternative or complement to survey data in the study of mobility behavior and activity analysis because of its relatively high access and low cost. In this paper, three OD estimation models have been utilized in order to investigate their relative performance when using Location-Based Social Networking (LBSN) data. For this, the Foursquare LBSN data was used to analyze the intra-urban movement behavioral patterns for the study area, Manhattan, the most densely populated of the five boroughs of New York city. The outputs of models are evaluated using real observations based on different criterions including distance distribution, destination travel constraints. The results demonstrate the promising potential of using LBSN data for urban travel demand analysis and monitoring.

  4. Development of an automatic method for americium and plutonium separation and preconcentration using an multisyringe flow injection analysis-multipumping flow system.

    Science.gov (United States)

    Fajardo, Yamila; Ferrer, Laura; Gómez, Enrique; Garcias, Francesca; Casas, Monserrat; Cerdà, Víctor

    2008-01-01

    A new procedure for automatic separation and preconcentration of 241Am and 239+240Pu from interfering matrixes using transuranide (TRU)-resin is proposed. Combination of the multisyringe flow injection analysis and multipumping flow system techniques with the TRU-resin allows carrying out the sampling treatment and separation in a short time using large sample volumes. Americium is eluted from the column with 4 mol L(-1) hydrochloric acid, and then plutonium is separated via on-column Pu(IV) reduction to Pu(III) with titanium(III) chloride. The corresponding alpha activities are measured off-line, with a relative standard deviation of 3% and a lower limit of detection of 0.004 Bq mL(-1), by using a multiplanchet low-background proportional counter.

  5. Measurement of Separated Flow Structures Using a Multiple-Camera DPIV System. [conducted in the Langley Subsonic Basic Research Tunnel

    Science.gov (United States)

    Humphreys, William M., Jr.; Bartram, Scott M.

    2001-01-01

    A novel multiple-camera system for the recording of digital particle image velocimetry (DPIV) images acquired in a two-dimensional separating/reattaching flow is described. The measurements were performed in the NASA Langley Subsonic Basic Research Tunnel as part of an overall series of experiments involving the simultaneous acquisition of dynamic surface pressures and off-body velocities. The DPIV system utilized two frequency-doubled Nd:YAG lasers to generate two coplanar, orthogonally polarized light sheets directed upstream along the horizontal centerline of the test model. A recording system containing two pairs of matched high resolution, 8-bit cameras was used to separate and capture images of illuminated tracer particles embedded in the flow field. Background image subtraction was used to reduce undesirable flare light emanating from the surface of the model, and custom pixel alignment algorithms were employed to provide accurate registration among the various cameras. Spatial cross correlation analysis with median filter validation was used to determine the instantaneous velocity structure in the separating/reattaching flow region illuminated by the laser light sheets. In operation the DPIV system exhibited a good ability to resolve large-scale separated flow structures with acceptable accuracy over the extended field of view of the cameras. The recording system design provided enhanced performance versus traditional DPIV systems by allowing a variety of standard and non-standard cameras to be easily incorporated into the system.

  6. Kinetically driven self-assembly of a binary solute mixture with controlled phase separation via electro-hydrodynamic flow of corona discharge.

    Science.gov (United States)

    Jung, Hee Joon; Huh, June; Park, Cheolmin

    2012-10-21

    This feature article describes a new and facile process to fabricate a variety of thin films of non-volatile binary solute mixtures suitable for high performance organic electronic devices via electro-hydrodynamic flow of conventional corona discharge. Both Corona Discharge Coating (CDC) and a modified version of CDC, Scanning Corona Discharge Coating (SCDC), are based on utilizing directional electric flow, known as corona wind, of the charged uni-polar particles generated by corona discharge between a metallic needle and a bottom plate under a high electric field (5-10 kV cm(-1)). The electric flow rapidly spreads out the binary mixture solution on the bottom plate and subsequently forms a smooth and flat thin film in a large area within a few seconds. In the case of SCDC, the static movement of the bottom electrode on which a binary mixture solution is placed provides further control of thin film formation, giving rise to a film highly uniform over a large area. Interesting phase separation behaviors were observed including nanometer scale phase separation of a polymer-polymer binary mixture and vertical phase separation of a polymer-organic semiconductor mixture. Core-shell type phase separation of either polymer-polymer or polymer-colloidal nanoparticle binary mixtures was also developed with a periodically patterned microstructure when the relative location of the corona wind was controlled to a binary solution droplet on a substrate. We also demonstrate potential applications of thin functional films with controlled microstructures by corona coating to various organic electronic devices such as electroluminescent diodes, field effect transistors and non-volatile polymer memories.

  7. Location and extent of recently active lava flows on the eastern flank of Idunn Mons on Venus

    Science.gov (United States)

    D'Incecco, Piero; Mueller, Nils; Helbert, Joern; D'Amore, Mario

    2016-10-01

    The eastern flank of Idunn Mons, Imdr Regio's single large volcano, was identified in VIRTIS data as one of the regions with relatively high values of thermal emissivity at 1 μm wavelength. Our study intends to identify location and extent of the sources of such anomalies, thus the lava flows responsible for the relatively high emissivity observed by VIRTIS over the eastern flank of Idunn Mons. We perform a simulation iterating the geologic mapping made over Magellan radar images of the same area with modeling of the blurring caused by the scattering of the 1 μm radiation in the atmosphere. At every iteration, we map the lava flow units in the surroundings of Idunn Mons and we assign each unit an assumed value of emissivity. We observed a good match between the mapped flows and the clusters resulting from the consistency of the mapped lava flows through the ISO clustering analysis. We tested eight different configurations, calculating the total RMS error compared to VIRTIS observations. The best-fit configuration is that where we assigned high values of emissivity to the flank lava flows. Results also show a correlation between the ISO clustering analysis and the best-fit configuration. We reconstructed the post-eruption stratigraphy of the eastern flank of Idunn Mons, displaying the three flank lava flows units likely responsible for the relatively high 1 μm emissivity anomalies observed by VIRTIS. The average microwave emissivity provides a further evidence of the basaltic composition of the mapped lava flows.

  8. Numerical study of shear rate effect on unsteady flow separation from the surface of the square cylinder using structural bifurcation analysis

    Science.gov (United States)

    Ray, Rajendra K.; Kumar, Atendra

    2017-08-01

    In this paper, an incompressible two-dimensional shear flow past a square cylinder problem is investigated numerically using a higher order compact finite difference scheme. Simulations are presented for three sets of Reynolds numbers, 100, 200, and 500, with various shear parameter (K) values ranging from 0.0 to 0.4. The purpose of the present study is to elaborate the influence of shear rate on the vortex shedding phenomenon behind the square cylinder. The results presented here show that the vortex shedding phenomenon strongly depends on Re as well as K. The strength and size of vortices shed behind the cylinder vary as a function of Re and K. When K is larger than a critical value, the vortex shedding phenomenon has completely disappeared depending on the Reynolds number. Apart from the numerical study, a thorough theoretical investigation has been done by using a topology based structural bifurcation analysis for unsteady flow separations from the walls of the cylinder. Through this analysis, we study the exact locations of the bifurcation points associated with secondary and tertiary vortices with appropriate non-dimensional time of occurrence. To the best of our knowledge, this is the first time, a topological aspect based structural bifurcation analysis has been done to understand the vortex shedding phenomenon and flow separation for this problem.

  9. Numerical simulation of the phase separation in binary lipid membrane under the effect of stationary shear flow.

    Science.gov (United States)

    Chen, Xiao-Bo; Niu, Li-Sha; Shi, Hui-Ji

    2008-06-01

    A numerical simulation of the phase separation in binary lipid membrane under the effect of stationary shear flow is performed. We numerically solved the modified two-dimensional time-dependent Ginzburg-Landau (TDGL) equations with an external velocity term, employing the CDS (i.e., Cell Dynamical System) technique. In the present simulation, stationary shear flows with different shear rates are taken into account. The evolution process of the phase separation is illustrated macroscopically via the snapshot figures and simulated scattering patterns at several typical moments. For each case, the growth exponents of the characteristic domain sizes in both directions parallel and perpendicular to the flow are studied, and the domain area as well. Also, the behavior of the excess viscosity has been investigated, which is a peculiar rheological indicator of such a membrane system with domain structures.

  10. Separating twin images and locating the center of a microparticle in dense suspensions using correlations among reconstructed fields of two parallel holograms.

    Science.gov (United States)

    Ling, Hangjian; Katz, Joseph

    2014-09-20

    This paper deals with two issues affecting the application of digital holographic microscopy (DHM) for measuring the spatial distribution of particles in a dense suspension, namely discriminating between real and virtual images and accurate detection of the particle center. Previous methods to separate real and virtual fields have involved applications of multiple phase-shifted holograms, combining reconstructed fields of multiple axially displaced holograms, and analysis of intensity distributions of weakly scattering objects. Here, we introduce a simple approach based on simultaneously recording two in-line holograms, whose planes are separated by a short distance from each other. This distance is chosen to be longer than the elongated trace of the particle. During reconstruction, the real images overlap, whereas the virtual images are displaced by twice the distance between hologram planes. Data analysis is based on correlating the spatial intensity distributions of the two reconstructed fields to measure displacement between traces. This method has been implemented for both synthetic particles and a dense suspension of 2 μm particles. The correlation analysis readily discriminates between real and virtual images of a sample containing more than 1300 particles. Consequently, we can now implement DHM for three-dimensional tracking of particles when the hologram plane is located inside the sample volume. Spatial correlations within the same reconstructed field are also used to improve the detection of the axial location of the particle center, extending previously introduced procedures to suspensions of microscopic particles. For each cross section within a particle trace, we sum the correlations among intensity distributions in all planes located symmetrically on both sides of the section. This cumulative correlation has a sharp peak at the particle center. Using both synthetic and recorded particle fields, we show that the uncertainty in localizing the axial

  11. Numerical and Experimental Studies on the Separation Topology of the MVG Controlled Flow at M=2.5

    CERN Document Server

    Li, Qin; Lu, Ping; Pierce, Adam; Liu, Chaoqun; Lu, Frank

    2014-01-01

    In this paper, the implicitly implemented LES method and fifth order bandwidth optimized WENO scheme are used to make comprehensive studies on the separation topology of the MVG controlled flow at M=2.5 and Re{\\theta}=5760. Experiments are also made to verify the prediction of the computation. Analyses are conducted on three categories of the topology: the surface separation, cross-section separation and the three dimensional structure of the vortices. A complete description about the separation topology and a series of new findings are obtained. Among them, a pair of spiral point is first predicted by the computation and verified by the experiment. A corresponding new vortex model with 7 vortex tubes is presented also.

  12. Theoretical and Empirical Studies of the Basic Structure of Turbulent Shear Flows, Including Separated Flows and Effects of Wall Curvature.

    Science.gov (United States)

    1981-01-01

    in I newly’% f’ahri cated water test. Iaci ifY built expressly for this program. Heat Transfer Sfudy onI C’, neave Su.rfne Good progress has been made...TO A. LYRIO FOR FRASER B FLOW. (NOTE THI’S FLOW WAS NOT PREDICTED WELL BY ANY METHOD IN THE 19b8 AFOSR-4FP STANFORD CONFERENCE) FIGuRE t-2 --- _f0.O Hz

  13. Flow-through immunomagnetic separation system for waterborne pathogen isolation and detection: Application to Giardia and Cryptosporidium cell isolation

    Energy Technology Data Exchange (ETDEWEB)

    Ramadan, Qasem, E-mail: qasem.alramadan@epfl.ch [Bioelectronics Program, Institute of Microelectronics, 11 Science Park Road, Singapore 117685 (Singapore); Christophe, Lay; Teo, William; ShuJun, Li; Hua, Feng Han [Bioelectronics Program, Institute of Microelectronics, 11 Science Park Road, Singapore 117685 (Singapore)

    2010-07-12

    Simultaneous sample washing and concentration of two waterborne pathogen samples were demonstrated using a rotational magnetic system under continuous flow conditions. The rotation of periodically arranged small permanent magnets close to a fluidic channel carrying magnetic particle suspension allows the trapping and release of particles along the fluidic channel in a periodic manner. Each trapping and release event resembles one washing cycle. The performance of the magnetic separation system (MSS) was evaluated in order to test its functionality to isolate magnetic-labelled protozoan cells from filtered, concentrated tap water, secondary effluent water, and purified water. Experimental protocols described in US Environmental Protection Agency method 1623 which rely on the use of a magnetic particle concentrator, were applied to test and compare our continuous flow cell separation system to the standard magnetic bead-based isolation instruments. The recovery efficiencies for Giardia cysts using the magnetic tube holder and our magnetic separation system were 90.5% and 90.1%, respectively, from a tap water matrix and about 31% and 18.5%, respectively, from a spiked secondary effluent matrix. The recovery efficiencies for Cryptosporidium cells using the magnetic tube holder and our magnetic separation system were 90% and 83.3%, respectively, from a tap water matrix and about 38% and 36%, respectively, from a spiked secondary effluent matrix. Recoveries from all matrices with the continuous flow system were typically higher in glass tubing conduits than in molded plastic conduits.

  14. HLA-targeted flow cytometric sorting of blood cells allows separation of pure and viable microchimeric cell populations.

    Science.gov (United States)

    Drabbels, Jos J M; van de Keur, Carin; Kemps, Berit M; Mulder, Arend; Scherjon, Sicco A; Claas, Frans H J; Eikmans, Michael

    2011-11-10

    Microchimerism is defined by the presence of low levels of nonhost cells in a person. We developed a reliable method for separating viable microchimeric cells from the host environment. For flow cytometric cell sorting, HLA antigens were targeted with human monoclonal HLA antibodies (mAbs). Optimal separation of microchimeric cells (present at a proportion as low as 0.01% in artificial mixtures) was obtained with 2 different HLA mAbs, one targeting the chimeric cells and the other the background cells. To verify purity of separated cell populations, flow-sorted fractions of 1000 cells were processed for DNA analysis by HLA-allele-specific and Y-chromosome-directed real-time quantitative PCR assays. After sorting, PCR signals of chimeric DNA markers in the positive fractions were significantly enhanced compared with those in the presort samples, and they were similar to those in 100% chimeric control samples. Next, we demonstrate applicability of HLA-targeted FACS sorting after pregnancy by separating chimeric maternal cells from child umbilical cord mononuclear cells. Targeting allelic differences with anti-HLA mAbs with FACS sorting allows maximal enrichment of viable microchimeric cells from a background cell population. The current methodology enables reliable microchimeric cell detection and separation in clinical specimens.

  15. Spatial separation of groundwater flow paths from a multi-flow system by a simple mixing model using stable isotopes of oxygen and hydrogen as natural tracers

    Science.gov (United States)

    Nakaya, Shinji; Uesugi, Kenji; Motodate, Yusuke; Ohmiya, Isao; Komiya, Hiroyuki; Masuda, Harue; Kusakabe, Minoru

    2007-09-01

    Stable isotopes of oxygen and hydrogen have the potential to serve as tracers for both source and flow paths in a groundwater system. The ratios of stable isotopes of oxygen (δ18O) and hydrogen (δD) can be used as natural tracer parameters to separate multiflow groundwater paths by applying a simple inversion analysis method to determine the differences between observed and calculated δ18O and δD data in a simple mixing model. The model presented here assumes that the distribution of natural tracers in the steady state is governed by simple mixing between flow paths with a normal distribution of flow rate. When the inversion analysis and simple mixing model were applied to the multiflow system of the Matsumoto Basin, which is surrounded by Japanese alpine ranges, the end-members of the relationship between observed δ18O and δD could be separated spatially into specific groundwater flow paths in the multiflow system of shallow and deep groundwater flow paths.

  16. Migration of gluten under shear flow: influence of process parameters on separation behaviour

    NARCIS (Netherlands)

    Peighambardoust, S.H.; Goot, van der A.J.

    2010-01-01

    The effect of processing conditions on the shear-induced migration of starch and gluten was described. A shearing device was used to induce a separation of wheat dough into a gluten rich fraction and a starch phase. A two-stage mechanism for separation was observed: first local aggregation of

  17. Numerically Simulated Impact of Gas Prandtl Number and Flow Model on Efficiency of the Machine-less Energetic Separation Device

    Directory of Open Access Journals (Sweden)

    K. S. Egorov

    2015-01-01

    Full Text Available The presented paper regards the influence of one of similarity criteria – the Prandtl number of gas (Pr - on the efficiency of the machine-less energetic separation device (Leontiev pipe, using numerical modeling in ANSYS software. This device, equally as Rank-Hilsch and Hartman-Schprenger pipes, is designed to separate one gas flow into two flows with different temperatures. One flow (supersonic streams out of the pipe with a temperature higher than initial and the other (subsonic flows out with a temperature lower than initial. This direction of energetic separation is true if the Prandtl number is less than 1 that corresponds to gases.The Prandtl number affects the efficiency of running Leontiev pipe indirectly both through a temperature difference on which a temperature recovery factor has an impact and through a thermal conductivity coefficient that shows the impact of heat transfer intensity between gas and solid wall.The Prandtl number range in the course of research was from 0.1 to 0.7. The Prandtl number value equal to 0.7 corresponds to the air or pure gases (for example, inert argon gas. The Prandtl number equal to 0.2 corresponds to the mixtures of inert gases such as helium-xenon.The numerical modeling completed for the supersonic flow with Mach number 2.0 shows that efficiency of the machine-less energetic separation device has been increased approximately 2 times with the Prandtl number decreasing from 0.7 to 0.2. Moreover, for the counter-flow scheme this effect is a little higher due to its larger heat efficiency in comparison with the straight-flow one.Also, the research shows that the main problem for the further increase of the Leontiev pipe efficiency is a small value of thermal conductivity coefficient, which requires an intensification of the heat exchange, especially in the supersonic flow. It can be obtained, for example, by using a system of oblique shock waves in the supersonic channel.

  18. Numerical algorithm conjugating steady and transient, separated, compressible flow and a solid body having arbitrarily distributed heat sources

    Science.gov (United States)

    Degani, D.

    1984-01-01

    A numerical algorithm that is second-order accurate in time has been developed for the conjugated problem of a separated, compressible flow field and a conductive solid body. The full two-dimensional time-dependent Navier-Stokes equations are coupled with the time-dependent energy equation for the solid body and are solved simultaneously. using implicit algorithms. The energy equation for the solid body may include arbitrarily distributed heat sources. The algorithm has been exmined for the case of two-dimensional supersonic compression-corner interaction, with a heat source embedded in the wall in the vicinity of the separation bubble and the attached boundary layer. The effect of the heat source on the flow field is studied for steady and transient cases.

  19. URANS simulations of separated flow with stall cells over an NREL S826 airfoil

    DEFF Research Database (Denmark)

    Sarlak Chivaee, Hamid; Nishino, T.; Sørensen, Jens Nørkær

    2016-01-01

    A series of wind tunnel measurements and oil flow visualization was recently carried out at the Technical University of Denmark in order to investigate flow characteristics over a 14% thick NREL S826 airfoil at low Reynolds numbers. This paper aims at presenting numerical simulations of the same ...

  20. URANS simulations of separated flow with stall cells over an NREL S826 airfoil

    DEFF Research Database (Denmark)

    Sarlak Chivaee, Hamid; Nishino, T.; Sørensen, Jens Nørkær

    2016-01-01

    A series of wind tunnel measurements and oil flow visualization was recently carried out at the Technical University of Denmark in order to investigate flow characteristics over a 14% thick NREL S826 airfoil at low Reynolds numbers. This paper aims at presenting numerical simulations of the same ...

  1. A Performance Assessment of a Tactical Airborne Separation Assistance System using Realistic, Complex Traffic Flows

    Science.gov (United States)

    Smith, Jeremy C.; Neitzke, Kurt W.; Bussink, Frank J. L.

    2008-01-01

    This paper presents the results from a study that investigates the performance of aspects of an Airborne Separation Assistance System (ASAS) under varying demand levels using realistic traffic patterns. This study only addresses the tactical aspects of an ASAS using aircraft state data (latitude, longitude, altitude, heading and speed) to detect and resolve projected conflicts. The main focus of this paper is to determine the extent to which sole reliance on the proposed tactical ASAS can maintain aircraft separation at demand levels up to three times current traffic. The effect of mixing ASAS equipped aircraft with non-equipped aircraft that do not have the capability to self-separate is also investigated.

  2. Analyzing the influence of PEG molecular weight on the separation of PEGylated gold nanoparticles by asymmetric-flow field-flow fractionation.

    Science.gov (United States)

    Hansen, Matthew; Smith, Mackensie C; Crist, Rachael M; Clogston, Jeffrey D; McNeil, Scott E

    2015-11-01

    Polyethylene glycol (PEG) is an important tool for increasing the biocompatibility of nanoparticle therapeutics. Understanding how these potential nanomedicines will react after they have been introduced into the bloodstream is a critical component of the preclinical evaluation process. Hence, it is paramount that better methods for separating, characterizing, and analyzing these complex and polydisperse formulations are developed. We present a method for separating nominal 30-nm gold nanoparticles coated with various molecular weight PEG moieties that uses only phosphate-buffered saline as the mobile phase, without the need for stabilizing surfactants. The optimized asymmetric-flow field-flow fractionation technique using in-line multiangle light scattering, dynamic light scattering, refractive index, and UV-vis detectors allowed successful separation and detection of a mixture of nanoparticles coated with 2-, 5-, 10-, and 20-kDa PEG. The particles coated with the larger PEG species (10 and 20 kDa) were eluted at times significantly earlier than predicted by field-flow fractionation theory. This was attributed to a lower-density PEG shell for the higher molecular weight PEGylated nanoparticles, which allows a more fluid PEG surface that can be greater influenced by external forces. Hence, the apparent particle hydrodynamic size may fluctuate significantly depending on the overall density of the stabilizing surface coating when an external force is applied. This has considerable implications for PEGylated nanoparticles intended for in vivo application, as nanoparticle size is important for determining circulation times, accumulation sites, and routes of excretion, and highlights the importance and value of the use of secondary size detectors when one is working with complex samples in asymmetric-flow field-flow fractionation.

  3. Effect of trailing edge shape on the separated flow characteristics around an airfoil at low Reynolds number: A numerical study

    Science.gov (United States)

    Thomareis, Nikitas; Papadakis, George

    2017-01-01

    Direct numerical simulations of the flow field around a NACA 0012 airfoil at Reynolds number 50 000 and angle of attack 5° with 3 different trailing edge shapes (straight, blunt, and serrated) have been performed. Both time-averaged flow characteristics and the most dominant flow structures and their frequencies are investigated using the dynamic mode decomposition method. It is shown that for the straight trailing edge airfoil, this method can capture the fundamental as well as the subharmonic of the Kelvin-Helmholtz instability that develops naturally in the separating shear layer. The fundamental frequency matches well with relevant data in the literature. The blunt trailing edge results in periodic vortex shedding, with frequency close to the subharmonic of the natural shear layer frequency. The shedding, resulting from a global instability, has an upstream effect and forces the separating shear layer. Due to forcing, the shear layer frequency locks onto the shedding frequency while the natural frequency (and its subharmonic) is suppressed. The presence of serrations in the trailing edge creates a spanwise pressure gradient, which is responsible for the development of a secondary flow pattern in the spanwise direction. This pattern affects the mean flow in the near wake. It can explain an unexpected observation, namely, that the velocity deficit downstream of a trough is smaller than the deficit after a protrusion. Furthermore, the insertion of serrations attenuates the energy of vortex shedding by de-correlating the spanwise coherence of the vortices. This results in weaker forcing of the separating shear layer, and both the subharmonics of the natural frequency and the shedding frequency appear in the spectra.

  4. Direct flow separation strategy, to isolate no-carrier-added {sup 90}Nb from irradiated Mo or Zr targets

    Energy Technology Data Exchange (ETDEWEB)

    Radchenko, Valery; Roesch, Frank [Mainz Univ. (Germany). Inst. of Nuclear Chemistry; Filosofov, Dmitry V.; Dadakhanov, Jakhongir [Joint Institute of Nuclear Research, Dubna (Russian Federation). Dzhelepov Laboratory of Nuclear Problems; Karaivanov, Dimitar V. [Joint Institute of Nuclear Research, Dubna (Russian Federation). Dzhelepov Laboratory of Nuclear Problems; Bulgarian Academy of Sciences, Sofia (Bulgaria). Inst. for Nuclear Research and Nuclear Energy; Marinova, Atanaska [Joint Institute of Nuclear Research, Dubna (Russian Federation). Dzhelepov Laboratory of Nuclear Problems; Sofia Univ. (Bulgaria). Faculty of Chemistry and Pharmacy; Baimukhanova, Ayagoz [Joint Institute of Nuclear Research, Dubna (Russian Federation). Dzhelepov Laboratory of Nuclear Problems; Institute of Nuclear Physics of the Republic of Kazakhstan, Almaty (Kazakhstan)

    2016-11-01

    {sup 90}Nb has an intermediate half-life of 14.6 h, a high positron branching of 53% and optimal β{sup +} emission energy of only E{sub mean} 0.35 MeV per decay. These favorable characteristics suggest it may be a potential candidate for application in immuno-PET. Our recent aim was to conduct studies on distribution coefficients for Zr{sup IV} and Nb{sup V} in mixtures of HCl/H{sub 2}O{sub 2} and HCl/oxalic acid for anion exchange resin (AG 1 x 8) and UTEVA resin to develop a ''direct flow'' separation strategy for {sup 90}Nb. The direct flow concept refers to a separation accomplished using a single eluent on multiple columns, effectively streamlining the separation process and increasing the time efficiency. Finally, we also demonstrated that this separation strategy is applicable to the production of the positron emitter {sup 90}Nb via the irradiation of molybdenum targets and isolation of {sup 90}Nb from the irradiated molybdenum target.

  5. Separation and determination of aloperine, sophoridine, matrine and oxymatrine by combination of flow injection with microfluidic capillary electrophoresis.

    Science.gov (United States)

    Cheng, Yuqiao; Chen, Hongli; Li, Yuqin; Chen, Xingguo; Hu, Zhide

    2004-05-28

    A novel, rapid and accurate method for the separation and determination of aloperine (ALP), sophoridine (SRI), matrine (MT) and oxymatrine (OMT) has been developed by combination of flow injection (FI) with microfluidic capillary electrophoresis (CE) for the first time. In the present paper, a continuous sample introduction interface was described. The interface with an H-channel structure was produced using a non-lithographic approach. The H-channel structure was fixed on a planar plastic base utilizing a horizontal 6.5cm-long separation capillary with two vertical sidearm tubes on each end that served as inlet and outlet flow-through electrode reservoirs. The inlet reservoir also functioned as interface for coupling to the FI system. The buffer solution used was a 50mmoll(-1) borate solution with the pH adjusted to 8.80 with 2moll(-1) HCl. The performance of the system was demonstrated in the separation and determination of ALP, SRI, MT and OMT with UV detection at 215nm, achieving baseline separation within 2min. A series of samples was injected repeatedly without current interruption and subsequent rinsing, and the contents of these four bio-alkaloids in two marketed drugs were determined with satisfactory recovery by this proposed method.

  6. Mathematical modeling and analysis of a unified power flow controller: A comparison of two approaches in power flow studies and effects of UPFC location

    Energy Technology Data Exchange (ETDEWEB)

    Vural, A. Mete [Department of Electrical and Electronics Engineering, University of Gaziantep, Gaziantep 27310 (Turkey); Tuemay, Mehmet [Department of Electrical and Electronics Engineering, Cukurova University, Adana 01330 (Turkey)

    2007-10-15

    This paper is focused on the mathematical modeling of unified power flow controller (UPFC), which is an advanced and versatile member of flexible ac transmission systems (FACTS). The proposed model is for the implementation of the device in conventional Newton-Raphson (NR) power flow algorithm and in power system analysis software package (PSASP). The model, derived from two-voltage source representation, is presented and analyzed in detail. The model represents a more robust and feasible alternative to others, because it is able to take operational losses of UPFC into account. A program in Fortran-77 language has been written in order to extend conventional NR algorithm based on proposed model. The model has also been adapted into PSASP by means of user-defined modeling technique. Different computer simulation studies performed on IEEE 14-bus and IEEE 30-bus test systems are presented in the paper to test and compare the two approaches. A robust and reliable convergence of the power flow studies is guaranteed by implementing the two approaches with high convergence speeds. UPFC can be theoretically located anywhere along a transmission line. In this respect, also the effects of UPFC allocation on power system operation have been investigated in detail. (author)

  7. A numerical investigation on the vortex formation and flow separation of the oscillatory flow in jet pumps

    CERN Document Server

    Oosterhuis, Joris P; Wilcox, Douglas; van der Meer, Theo

    2015-01-01

    A two-dimensional computational fluid dynamics model is used to predict the oscillatory flow through a tapered cylindrical tube section (jet pump) placed in a larger outer tube. Due to the shape of the jet pump, there will exist an asymmetry in the hydrodynamic end effects which will cause a time-averaged pressure drop to occur that can be used to cancel Gedeon streaming in a closed-loop thermoacoustic device. The performance of two jet pump geometries with different taper angles is investigated. A specific time-domain impedance boundary condition is implemented in order to simulate traveling acoustic wave conditions. It is shown that by scaling the acoustic displacement amplitude to the jet pump dimensions, similar minor losses are observed independent of the jet pump geometry. Four different flow regimes are distinguished and the observed flow phenomena are related to the jet pump performance. The simulated jet pump performance is compared to an existing quasi-steady approximation which is shown to only be ...

  8. Separation of nanoparticles by a nano-orifice based DC-dielectrophoresis method in a pressure-driven flow.

    Science.gov (United States)

    Zhao, Kai; Peng, Ran; Li, Dongqing

    2016-12-07

    A novel DC-dielectrophoresis (DEP) method employing a pressure-driven flow for the continuous separation of micro/nano-particles is presented in this paper. To generate the DEP force, a small voltage difference is applied to produce a non-uniformity of the electric field across a microchannel via a larger orifice of several hundred microns on one side of the channel wall and a smaller orifice of several hundred nanometers on the opposite channel wall. The particles experience a DEP force when they move with the flow through the vicinity of the small orifice, where the strongest electrical field gradient exists. Experiments were conducted to demonstrate the separation of 1 μm and 3 μm polystyrene particles by size by adjusting the applied electrical potentials. In order to separate smaller nanoparticles, the electrical conductivity of the suspending solution is adjusted so that the polystyrene nanoparticles of a given size experience positive DEP while the polystyrene nanoparticles of another size experience negative DEP. Using this method, the separation of 51 nm and 140 nm nanoparticles and the separation of 140 nm and 500 nm nanoparticles were demonstrated. In comparison with the microfluidic DC-DEP methods reported in the literature which utilize hurdles or obstacles to induce the non-uniformity of an electric field, a pair of asymmetrical orifices on the channel side walls is used in this method to generate a strong electrical field gradient and has advantages such as capability of separating nanoparticles, and locally applied lower electrical voltages to minimize the Joule heating effect.

  9. Factors influencing base flow in the Swiss Midlands - Can results from different base flow separation methods help to identify these factors?

    Science.gov (United States)

    Meyer, Raphael; Schädler, Bruno; Viviroli, Daniel; Weingartner, Rolf

    2010-05-01

    Base flow is a desirable entity to know, for water management in general and particularly for climate change impact studies. Base flow is most often defined as that part of total discharge which origins from delayed storages in a river catchment. During a prolonged period without rain, base flow is the sole contributor to discharge. Base flow therefore makes a river perennial. A high base flow contribution to total annual discharge makes a river more stable in respect of meteorological droughts. Annual base flow from a catchment cannot be determined exactly. Only total discharge can be measured with high accuracy. Therefore, base flow has to be estimated with appropriate methods. Calculating an entity which cannot be verified by measurements is easy. By defining the entity with a calculation procedure, the result is numerically always right. It is actually much more difficult to understand the results, i.e. how these outcomes should be interpreted. The present study investigates the application of three different base flow separation procedures for numerous (up to 40) meso-scale catchments in Switzerland. The methods Demuth (1993), Wittenberg (1999) and Institute of Hydrology (1980) are different approaches to determine base flow, based on daily runoff data. The method Demuth, and the separation of base flow according to Institute of Hydrology, are statistical methods. Demuth is based on the graphical approach of Kille (1970), and the procedure of the Institute of Hydrology is an empirical smoothing method. In contrast to this, the method by Wittenberg does not presume linearity between storage and outflow. Analyzing the results, among each other and in comparison with physiographic characteristics of the catchments under consideration, leads to a more detailed picture of the ongoing processes. At least the dominant control factors for base flow in the Swiss Midlands should be detectable. These are expected to be found first of all among geology and climate, which

  10. Continuous particle separation in spiral microchannels using Dean flows and differential migration.

    Science.gov (United States)

    Bhagat, Ali Asgar S; Kuntaegowdanahalli, Sathyakumar S; Papautsky, Ian

    2008-11-01

    Microparticle separation and concentration based on size has become indispensable in many biomedical and environmental applications. In this paper we describe a passive microfluidic device with spiral microchannel geometry for complete separation of particles. The design takes advantage of the inertial lift and viscous drag forces acting on particles of various sizes to achieve differential migration, and hence separation, of microparticles. The dominant inertial forces and the Dean rotation force due to the spiral microchannel geometry cause the larger particles to occupy a single equilibrium position near the inner microchannel wall. The smaller particles migrate to the outer half of the channel under the influence of Dean forces resulting in the formation of two distinct particle streams which are collected in two separate outputs. This is the first demonstration that takes advantage of the dual role of Dean forces for focusing larger particles in a single equilibrium position and transposing the smaller particles from the inner half to the outer half of the microchannel cross-section. The 5-loop spiral microchannel 100 microm wide and 50 microm high was used to successfully demonstrate a complete separation of 7.32 microm and 1.9 microm particles at Dean number De = 0.47. Analytical analysis supporting the experiments and models is also presented. The simple planar structure of the separator offers simple fabrication and makes it ideal for integration with on-chip microfluidic systems, such as micro total analysis systems (muTAS) or lab-on-a-chip (LOC) for continuous filtration and separation applications.

  11. Appliance of Inertial Gas-Dynamic Separation of Gas-Dispersion Flows in the Curvilinear Convergent-Divergent Channels for Compressor Equipment Reliability Improvement

    Science.gov (United States)

    Liaposhchenko, O. O.; Sklabinskyi, V. I.; Zavialov, V. L.; Pavlenko, I. V.; Nastenko, O. V.; Demianenko, M. M.

    2017-08-01

    The new methods of vibration and inertial gas-dynamic separation of gas-condensate and dusty flows and the corresponding separation devices are proposed in order to avoid emergencies and premature wear of parts and components of the compressor equipment. The formation of the gas flow and disperse particles in the curvilinear convergent-divergent channels are investigated. The optimizing hydrodynamic profiling of a geometrical configuration of curvilinear separation channels with rigid and flexible walls of baffles is carried out.

  12. AM06-021-007 A LES Study on Scalar Transport in Separated Turbulent Flows

    OpenAIRE

    反町, 洋之; 松原, 幸治; 高野, 哲夫; 杉浦, 聡; 小林, 睦夫; Hiroyuki, Sorimachi; Koji, MATSUBARA; Tetsuo, TAKANO; Satoshi, Sugiura; Mutsuo, Kobayashi; GISP; 新潟大; GIS Planners Inc.; Niigata Univ.

    2006-01-01

    Large eddy simulation was performed for lateral transport of scalar in a backward-facing step flow. Inlet profile is assumed to be fully developed turbulent channel flow having spanwise non-uniformity of mean temperature. Reynolds number based on center velocity and step height is set at 6,640, and Prandtl number at 0.71. Numerical validity was confirmed through comparison between numerical data and PTV measurement by Kasagi et al. Spanwise eddy diffusivity of heat was consistent with spanwis...

  13. Inverstigation on the Separated Turbulent Flow Field in Dual Rectangular Jets

    Institute of Scientific and Technical Information of China (English)

    TANFa-sheng; LIUJie-wei; 等

    2001-01-01

    In the present paper,the flow field of dual rectangular jets was numerically simulated by solving the full Reynolds averaged Navier-Stokes equations,where the RNG κ-ε model and the finite volume method were used.The flow structure in dual rectangular jets and the effects of the velocity were investigated.The numerical results agree qualitatively with the experimental data.

  14. 梭锥管混浊流体分离装置流场PIV测试及分析%Test and analysis on flow field in the shuttle-conical tube turbid flow hydraulic separation Device by PIV techniques

    Institute of Scientific and Technical Information of China (English)

    李琳; 杨海华; 王苗; 邱秀云

    2013-01-01

    Particle image uelocimotrg (PIV) was used to test the water and sediment flow velocity field in the Shuttle-conical Tube Turbid Flow hydraulic separation Device (SCT) with two cases. The distributions of sediment flow velocity vector and the velocity diagram at various planes in different locations of the SCT have been offered. The results indicate that the test results are consistent with the physical experimental phenomenon and theoretical analysis. The SCT is divided into many sedimentation spaces by multilayer cone rings so that the sedimentation distance of SCT is shorter than the common device without any cone ring and all the sedimentation spaces are independent from each other. The turbid flow with suspension flow into the SCT, and sediment free settlement distance has been shortened, and in the water-sediment separation space composed by adjacent two cone rings,the sediment flow is downward along the upper sur-face of one cone ring, and the clear water flow is upward along the lower surface of the other cone ring. Then the water and sediment flow respectively along different path, the former flows into the clear water passage arranged at the sides of the wall, and the latter flows into the sand drainage channel at the center of the SCT. In the process of the separation and drainage, the clear water doesn’t mix and interfere with sediment flow so that the separation efficiency of water & sediment by the SCT has been improved.%利用粒子图像测速技术,测试了两种工况下梭锥管混浊流体分离装置(简称梭锥管)内的水沙两相流的速度场,给出了梭锥管内泥沙运动的流速矢量分布及流速大小云图。对测试结果的分析表明,实测结果与理论分析结果一致。含沙水流进入梭锥管后,其内设置的多层锥圈把泥沙沉降区域分割成若干个沉降距离较短且相互独立的沉降空间,缩短了泥沙的自由沉降距离。相邻锥圈组成的水沙分离空间内,形

  15. Computing daily mean streamflow at ungaged locations in Iowa by using the Flow Anywhere and Flow Duration Curve Transfer statistical methods

    Science.gov (United States)

    Linhart, S. Mike; Nania, Jon F.; Sanders, Curtis L.; Archfield, Stacey A.

    2012-01-01

    The U.S. Geological Survey (USGS) maintains approximately 148 real-time streamgages in Iowa for which daily mean streamflow information is available, but daily mean streamflow data commonly are needed at locations where no streamgages are present. Therefore, the USGS conducted a study as part of a larger project in cooperation with the Iowa Department of Natural Resources to develop methods to estimate daily mean streamflow at locations in ungaged watersheds in Iowa by using two regression-based statistical methods. The regression equations for the statistical methods were developed from historical daily mean streamflow and basin characteristics from streamgages within the study area, which includes the entire State of Iowa and adjacent areas within a 50-mile buffer of Iowa in neighboring states. Results of this study can be used with other techniques to determine the best method for application in Iowa and can be used to produce a Web-based geographic information system tool to compute streamflow estimates automatically. The Flow Anywhere statistical method is a variation of the drainage-area-ratio method, which transfers same-day streamflow information from a reference streamgage to another location by using the daily mean streamflow at the reference streamgage and the drainage-area ratio of the two locations. The Flow Anywhere method modifies the drainage-area-ratio method in order to regionalize the equations for Iowa and determine the best reference streamgage from which to transfer same-day streamflow information to an ungaged location. Data used for the Flow Anywhere method were retrieved for 123 continuous-record streamgages located in Iowa and within a 50-mile buffer of Iowa. The final regression equations were computed by using either left-censored regression techniques with a low limit threshold set at 0.1 cubic feet per second (ft3/s) and the daily mean streamflow for the 15th day of every other month, or by using an ordinary-least-squares multiple

  16. A United Method for Sensitivity Analysis of the Locational Marginal Price Based on the Optimal Power Flow

    Directory of Open Access Journals (Sweden)

    Liu Yang

    2014-01-01

    Full Text Available Locational marginal prices (LMPs are influenced by various factors in the electricity market; knowing the sensitivity information of LMPs is very important for both the purchase and the consumer. This paper presents a united method to compute the sensitivities of LMPs based on the optimal power flow (OPF. The Karush-Kuhn-Tucher (KKT system to solve LMPs can be transferred into an equation system by using an NCP function, and then by using the properties of the derivative of the semismooth NCP function, this paper provides a simultaneous obtention of the sensitivities of LMPs with respect to power demands, the cost of production, voltage boundary, and so forth. Numerical examples illustrate the concepts presented and the proposed methodology by a 6-bus electric energy system. Some relevant conclusions are drawn in the end.

  17. Genetic Algorithm for Solving Location Problem in a Supply Chain Network with Inbound and Outbound Product Flows

    Directory of Open Access Journals (Sweden)

    Suprayogi Suprayogi

    2016-12-01

    Full Text Available This paper considers a location problem in a supply chain network. The problem addressed in this paper is motivated by an initiative to develop an efficient supply chain network for supporting the agricultural activities. The supply chain network consists of regions, warehouses, distribution centers, plants, and markets. The products include a set of inbound products and a set of outbound products. In this paper, definitions of the inbound and outbound products are seen from the region’s point of view.  The inbound product is the product demanded by regions and produced by plants which flows on a sequence of the following entities: plants, distribution centers, warehouses, and regions. The outbound product is the product demanded by markets and produced by regions and it flows on a sequence of the following entities: regions, warehouses, and markets. The problem deals with determining locations of the warehouses and the distribution centers to be opened and shipment quantities associated with all links on the network that minimizes the total cost. The problem can be considered as a strategic supply chain network problem. A solution approach based on genetic algorithm (GA is proposed. The proposed GA is examined using hypothetical instances and its results are compared to the solution obtained by solving the mixed integer linear programming (MILP model. The comparison shows that there is a small gap (0.23%, on average between the proposed GA and MILP model in terms of the total cost. The proposed GA consistently provides solutions with least total cost. In terms of total cost, based on the experiment, it is demonstrated that coefficients of variation are closed to 0.

  18. An instrument to control parallel plate separation for nanoscale flow control

    Science.gov (United States)

    White, J.; Ma, H.; Lang, J.; Slocum, A.

    2003-11-01

    The handling of extremely small samples of gases and liquids has long been a subject of research among biologists, chemists, and engineers. A few scientific instruments, notably the surface force apparatus, have been used extensively to investigate very short-range molecular phenomena. This article describes the design, fabrication, and characterization of an easily manufactured, gas and liquid flow control device called the Nanogate. The Nanogate controls liquid flows under very high planar confinement, wherein the liquid film is, in one dimension, on the scale of nanometers, but is on the scale of hundreds of microns in its other dimensions. The liquid film is confined between a silica (Pyrex) surface with a typical roughness of Ra≈6 nm and a gold-covered silicon surface with a typical roughness of Ra≈2 nm. During the manufacturing process, the Pyrex flows and conforms to the gold-covered silicon surface, improving the mating properties of the two surfaces. The fluid film thickness can be controlled within 2 Å, from sub-10 nm up to 1 μm. Control of helium gas flow rates in the 10-9 atm cm3/s range, and sub-nl/s flow rates of water and methanol have been predicted and experimentally verified.

  19. Effect of the Flow Channel Structure on the Nanofiltration Separation Performance

    Directory of Open Access Journals (Sweden)

    Zhi Chen

    2013-01-01

    Full Text Available Two kinds of newly designed feed channels, for example, a spiral and a serpentine feed channels, for a bench-scale nanofiltration module were developed to improve the filtration performance. The experiments were carried out with the modules using a commercial flat NF membrane to investigate the effects of Reynolds number (Re and flow channel structures on the flux of permeate and Mg2+ rejection. It was shown from the experimental results that although the effects of Reynolds number on fluxes were not obvious for the two new feed channels compared with a normal flow channel structure, the Mg2+ rejections varied apparently with Re. The Mg2+ rejections were almost the same for the modules with two new feed channels and larger than that for the module with normal feed channel. The numerical simulations of fluid flow in the three kinds of feed channels were completed at Re of 4800 to explain the phenomena. The results demonstrated that there was a secondary flow in both new feed channels, which strongly influences the Mg2+ rejection. The rejection increased with increasing average shear stress at the membrane wall. The spiral feed channel was the best one among the flow channel structures investigated.

  20. Development of a centrifugal in-line separator for oil-water flows

    NARCIS (Netherlands)

    Slot, J.J.

    2013-01-01

    The world energy consumption will increase in the next decades. However, many aging oil fields are showing a steady decline in oil production. And they are producing increasing amounts of water, making the separation of the oil from the oil-water mixture an important processing step. In-line separa

  1. SIMULTANEOUS FLOW VISUALIZATION AND WALL-PRESSURE MEASUREMENT OF THE TURBULENT SEPARATED AND REATTACHING FLOW OVER A BACKWARD-FACING STEP

    Institute of Scientific and Technical Information of China (English)

    KE Feng; LIU Ying-zheng; CHEN Han-ping; HIDE S. Koyama

    2007-01-01

    Unsteady behaviors of the large-scale vortical structure superimposed in the turbulent separated and reattaching flow over a backward-facing step were convincingly delineated by performing simultaneous measurements of the wall pressure fluctuations and visualizations of the flow. Toward this end, a synchronized instrumentation system integrated with the microphone array and the high-speed camera was established. The smoke-wire technique was employed to visualize the unsteady events. A thorough analysis based on the wall pressure fluctuations disclosed that the large-scale vortical structure shedding at the frequency of = 0.064 gave a primary contribution to the wall pressure fluctuations, and consequently dominated unsteady behaviors of the turbulent shear layer. The convection velocity of the large-scale vortical structure was determined as =0.55. The instantaneous flow visualizations and wall pressure were compared in a straightforward manner. Below the separation bubble and the reattachment zone, the negative peak of the time-varying wall pressure was in phase with passage of the local large-scale vortical structure. In the redeveloping turbulent boundary layer, the decaying large-scale vortical structure was clearly revealed.

  2. Navier-Stokes computations of separated vortical flows past prolate spheroid at incidence

    Science.gov (United States)

    Wong, Tin-Chee; Kandil, Osama A.; Liu, C. H.

    1989-01-01

    The problem of steady incompressible viscous flow past prolate spheroids at incidence is formulated using the unsteady incompressible and compressible thin-layer Navier-Stokes equations. The two sets of Navier-Stokes equations are solved using a pseudotime stepping of the implicit flux-difference splitting scheme on a curvilinear grid, which is generated by a transfinite grid generator. The Baldwin and Lomax (1978) algebraic eddy-viscosity model is used to model the turbulent flow. The computational applications cover a 6:1 prolate spheroid at different angles of attack and Reynolds numbers. The results are compared with experimental data.

  3. Passive and Active Control of Massively Separated High-Speed Flows

    Science.gov (United States)

    2014-01-31

    J052022 Bradley DeBlauw , Bradley Sanders, Nick Glumac, Craig Dutton, Gregory Elliott. Correlation Between Emission, Electric, and Flow Properties of Arc...2012 08/16/2011 18.00 11.00 13.00 12.00 Received Paper 9.00 5.00 Bradley G. DeBlauw , Eli Lazar, Nachiket Kale, Nick Glumac, Craig Dutton, Gregory Elliott...Flow and Thermal Properties Induced by Electric Arc Plasma Actuators, 2011 AIAA Aerospace Sciences Meeting. 04-JAN-11, . : , Bradley DeBlauw

  4. Strongly polynomial algorithm for a class of minimum-cost flow problems with separable convex objectives

    OpenAIRE

    Vegh, Laszlo A.

    2011-01-01

    A well-studied nonlinear extension of the minimum-cost flow problem is to minimize the objective $\\sum_{ij\\in E} C_{ij}(f_{ij})$ over feasible flows $f$, where on every arc $ij$ of the network, $C_{ij}$ is a convex function. We give a strongly polynomial algorithm for the case when all $C_{ij}$'s are convex quadratic functions, settling an open problem raised e.g. by Hochbaum [1994]. We also give strongly polynomial algorithms for computing market equilibria in Fisher markets with linear util...

  5. Quantitative investigation of resolution increase of free-flow electrophoresis via simple interval sample injection and separation.

    Science.gov (United States)

    Shao, Jing; Fan, Liu-Yin; Cao, Cheng-Xi; Huang, Xian-Qing; Xu, Yu-Quan

    2012-07-01

    Interval free-flow zone electrophoresis (FFZE) has been used to suppress sample band broadening greatly hindering the development of free-flow electrophoresis (FFE). However, there has been still no quantitative study on the resolution increase of interval FFZE. Herein, we tried to make a comparison between bandwidths in interval FFZE and continuous one. A commercial dye with methyl green and crystal violet was well chosen to show the bandwidth. The comparative experiments were conducted under the same sample loading of the model dye (viz. 3.49, 1.75, 1.17, and 0.88 mg/h), the same running time (viz. 5, 10, 15, and 20 min), and the same flux ratio between sample and background buffer (= 10.64 × 10⁻³). Under the given conditions, the experiments demonstrated that (i) the band broadening was evidently caused by hydrodynamic factor in continuous mode, and (ii) the interval mode could clearly eliminate the hydrodynamic broadening existing in continuous mode, greatly increasing the resolution of dye separation. Finally, the interval FFZE was successfully used for the complete separation of two-model antibiotics (herein pyoluteorin and phenazine-1-carboxylic acid coexisting in fermentation broth of a new strain Pseudomonas aeruginosa M18), demonstrating the feasibility of interval FFZE mode for separation of biomolecules.

  6. High Re Separated Flow Solutions Using the Navier-Stokes and Approximate Equations,

    Science.gov (United States)

    1985-01-01

    flow field. In the present study the incremental block-line Gauss- Seidel method proposed in Ref. 17 is used as an efficient numerical tool for solving...Incremental Block-Line-Gauss- Seidel Method for the Navier-Stokes Equations", AIAA Paper 85-0033. 18. Beam, R. M. and Warming, R. F., "An Implicit Factored

  7. Comparison of different base flow separation methods in a lowland catchment

    NARCIS (Netherlands)

    Gonzales, A.L.; Nonner, J.; Heijkers, J.; Uhlenbrook, S.

    2009-01-01

    Assessment of water resources available in different storages and moving along different pathways in a catchment is important for its optimal use and protection, and also for the prediction of floods and low flows. Moreover, understanding of the runoff generation processes is essential for assessing

  8. Numerical modeling of separated flows at moderate Reynolds numbers appropriate for turbine blades and unmanned aero vehicles

    Science.gov (United States)

    Castiglioni, Giacomo

    Flows over airfoils and blades in rotating machinery, for unmanned and micro-aerial vehicles, wind turbines, and propellers consist of a laminar boundary layer near the leading edge that is often followed by a laminar separation bubble and transition to turbulence further downstream. Typical Reynolds averaged Navier-Stokes turbulence models are inadequate for such flows. Direct numerical simulation is the most reliable, but is also the most computationally expensive alternative. This work assesses the capability of immersed boundary methods and large eddy simulations to reduce the computational requirements for such flows and still provide high quality results. Two-dimensional and three-dimensional simulations of a laminar separation bubble on a NACA-0012 airfoil at Rec = 5x104 and at 5° of incidence have been performed with an immersed boundary code and a commercial code using body fitted grids. Several sub-grid scale models have been implemented in both codes and their performance evaluated. For the two-dimensional simulations with the immersed boundary method the results show good agreement with the direct numerical simulation benchmark data for the pressure coefficient Cp and the friction coefficient Cf, but only when using dissipative numerical schemes. There is evidence that this behavior can be attributed to the ability of dissipative schemes to damp numerical noise coming from the immersed boundary. For the three-dimensional simulations the results show a good prediction of the separation point, but an inaccurate prediction of the reattachment point unless full direct numerical simulation resolution is used. The commercial code shows good agreement with the direct numerical simulation benchmark data in both two and three-dimensional simulations, but the presence of significant, unquantified numerical dissipation prevents a conclusive assessment of the actual prediction capabilities of very coarse large eddy simulations with low order schemes in general

  9. Separation and determination of four active anthraquinones in Chinese herbal preparations by flow injection-capillary electrophoresis.

    Science.gov (United States)

    Liu, Lihong; Fan, Liuyin; Chen, Hongli; Chen, Xingguo; Hu, Zhide

    2005-08-01

    A simple, rapid, and accurate method for the separation and determination of physcion, chrysophanol, aloe-emodin, and emodin in Rhubarb, Juemingzi, and Chinese herbal preparations was developed by combination of flow injection-capillary zone electrophoresis for the first time. The analysis was carried out using an unmodified fused-silica capillary (75 mm x 50 microm ID x 375 microm OD, effective separation length of 48 mm) and direct ultraviolet detection at 254 nm. By a series of optimization, the sample solvent consisted of NaOH (100 mmol/L) and ACN (1:1 v/v), and a running buffer composed of 15 mmol/L sodium borate - 12.5 mmol/L sodium dihydrogen phosphate - 42% v/v ACN (pH 10.1) was applied for the separation of the four anthraquinones. The separation was rapid and highly reproducible, with complete resolution of all four compounds within 6 min. The sample throughput rate could reach up to 12 per h. The repeatability (defined as relative standard deviation) was 4.45, 4.44, 4.34, 0.61% with peak height evaluation and 1.62, 0.89, 2.49, 2.19% with peak area evaluation for physcion, chrysophanol, aloe-emodin, and emodin, respectively.

  10. Phenomenological aspects of quasi-stationary controlled and uncontrolled three-dimensional flow separations. [in relation to aircraft design considerations and swept wings

    Science.gov (United States)

    Peake, D. J.

    1978-01-01

    Quasi-steady three dimensional separated flows about bodies of large fineness ratio operating at large angles of incidence or yaw are discussed. The general character of the three dimensional attached boundary layer, the concept of limiting streamlines, and the physics of three dimensional separation and reattachment are among the factors considered. Specific examples are given. The advantages of swept, sharp edges that generate controlled (or fixed) three dimensional flow separations on a vehicle, due to the qualitatively unchanging flow field developed throughout the range of flight conditions, are emphasized.

  11. Effects of Input Voltage on Flow Separation Control for Low-Pressure Turbine at Low Reynolds Number by Plasma Actuators

    Directory of Open Access Journals (Sweden)

    Takayuki Matsunuma

    2012-01-01

    Full Text Available Active flow control using dielectric barrier discharge (DBD plasma actuators was investigated to reattach the simulated boundary layer separation on the suction surface of a turbine blade at low Reynolds number, Re = 1.7 × 104. The flow separation is induced on a curved plate installed in the test section of a low-speed wind tunnel. Particle image velocimetry (PIV was used to obtain instantaneous and time-averaged two-dimensional velocity measurements. The amplitude of input voltage for the plasma actuator was varied from ±2.0 kV to ±2.8 kV. The separated flow reattached on the curved wall when the input voltage was ±2.4 kV and above. The displacement thickness of the boundary layer near the trailing edge decreased by 20% at ±2.0 kV. The displacement thickness was suddenly reduced as much as 56% at ±2.2 kV, and it was reduced gradually from ±2.4 kV to ±2.8 kV (77% reduction. The total pressure loss coefficient, estimated from the boundary layer displacement thickness and momentum thickness, was 0.172 at the baseline (actuator off condition. The total pressure loss was reduced to 0.107 (38% reduction at ±2.2 kV and 0.078 (55% reduction at ±2.8 kV.

  12. "Reagentless" flow injection determination of ammonia and urea using membrane separation and solid phase basification

    Science.gov (United States)

    Akse, J. R.; Thompson, J. O.; Sauer, R. L.; Atwater, J. E.

    1998-01-01

    Flow injection analysis instrumentation and methodology for the determination of ammonia and ammonium ions in an aqueous solution are described. Using in-line solid phase basification beds containing crystalline media. the speciation of ammoniacal nitrogen is shifted toward the un-ionized form. which diffuses in the gas phase across a hydrophobic microporous hollow fiber membrane into a pure-water-containing analytical stream. The two streams flow in a countercurrent configuration on opposite sides of the membrane. The neutral pH of the analytical stream promotes the formation of ammonium cations, which are detected using specific conductance. The methodology provides a lower limit of detection of 10 microgram/L and a dynamic concentration range spanning three orders of magnitude using a 315-microliters sample injection volume. Using immobilized urease to enzymatically promote the hydrolysis of urea to produce ammonia and carbon dioxide, the technique has been extended to the determination of urea.

  13. Membrane Separator for Redox Flow Batteries that Utilize Anion Radical Mediators.

    Energy Technology Data Exchange (ETDEWEB)

    Delnick, Frank M.

    2014-10-01

    A Na + ion conducting polyethylene oxide membrane is developed for an organic electrolyte redox flow battery that utilizes anion radical mediators. To achieve high specific ionic conductivity, tetraethyleneglycol dimethylether (TEGDME) is used as a plasticizer to reduce crystallinity and increase the free volume of the gel film. This membrane is physically and chemically stable in TEGDME electrolyte that contains highly reactive biphenyl anion radical mediators.

  14. Removing flow backgrounds from the charge-separation observable perpendicular to the reaction plane in heavy-ion collisions

    CERN Document Server

    Wen, Fufang; Wang, Gang

    2016-01-01

    Recent charge-dependent azimuthal correlation measurements in high-energy heavy-ion collisions have observed charge-separation signals perpendicular to the reaction plane, and the observations have been related to the chiral magnetic effect (CME). However, the correlation signal is contaminated with the background contributions due to the collective motion (flow) of the collision system, and it remains elusive to effectively remove the background from the correlation. We present a method study with Monte Carlo simulations and a multi-phase transport model, and develop a scheme to reveal the true CME signal via the event-shape engineering with the flow vector, $\\overrightarrow{q}$. An alternative approach using the ensemble averages of observables is also discussed.

  15. Separate base usages of genes located on the leading and lagging strands in Chlamydia muridarum revealed by the Z curve method

    Directory of Open Access Journals (Sweden)

    Yu Xiu-Juan

    2007-10-01

    Full Text Available Abstract Background The nucleotide compositional asymmetry between the leading and lagging strands in bacterial genomes has been the subject of intensive study in the past few years. It is interesting to mention that almost all bacterial genomes exhibit the same kind of base asymmetry. This work aims to investigate the strand biases in Chlamydia muridarum genome and show the potential of the Z curve method for quantitatively differentiating genes on the leading and lagging strands. Results The occurrence frequencies of bases of protein-coding genes in C. muridarum genome were analyzed by the Z curve method. It was found that genes located on the two strands of replication have distinct base usages in C. muridarum genome. According to their positions in the 9-D space spanned by the variables u1 – u9 of the Z curve method, K-means clustering algorithm can assign about 94% of genes to the correct strands, which is a few percent higher than those correctly classified by K-means based on the RSCU. The base usage and codon usage analyses show that genes on the leading strand have more G than C and more T than A, particularly at the third codon position. For genes on the lagging strand the biases is reverse. The y component of the Z curves for the complete chromosome sequences show that the excess of G over C and T over A are more remarkable in C. muridarum genome than in other bacterial genomes without separating base and/or codon usages. Furthermore, for the genomes of Borrelia burgdorferi, Treponema pallidum, Chlamydia muridarum and Chlamydia trachomatis, in which distinct base and/or codon usages have been observed, closer phylogenetic distance is found compared with other bacterial genomes. Conclusion The nature of the strand biases of base composition in C. muridarum is similar to that in most other bacterial genomes. However, the base composition asymmetry between the leading and lagging strands in C. muridarum is more significant than that in

  16. Separation and characterization of nanoparticles in complex food and environmental samples by field-flow fractionation

    DEFF Research Database (Denmark)

    Kammer, Frank von der; Legros, Samuel; Hofmann, Thilo

    2011-01-01

    The thorough analysis of natural nanoparticles (NPs) and engineered NPs involves the sequence of detection, identification, quantification and, if possible, detailed characterization. In a complex or heterogeneous sample, each step of this sequence is an individual challenge, and, given suitable...... has been applied for separation of various types of NP (e.g., organic macromolecules, and carbonaceous or inorganic NPs) in different types of media (e.g., natural waters, soil extracts or food samples).FFF can be coupled to different types of detectors that offer additional information...

  17. How the python heart separates pulmonary and systemic blood pressures and blood flows.

    Science.gov (United States)

    Jensen, Bjarke; Nielsen, Jan M; Axelsson, Michael; Pedersen, Michael; Löfman, Carl; Wang, Tobias

    2010-05-01

    The multiple convergent evolution of high systemic blood pressure among terrestrial vertebrates has always been accompanied by lowered pulmonary pressure. In mammals, birds and crocodilians, this cardiac separation of pressures relies on the complete division of the right and left ventricles by a complete ventricular septum. However, the anatomy of the ventricle of most reptiles does not allow for complete anatomical division, but the hearts of pythons and varanid lizards can produce high systemic blood pressure while keeping the pulmonary blood pressure low. It is also known that these two groups of reptiles are characterised by low magnitudes of cardiac shunts. Little, however, is known about the mechanisms that allow for this pressure separation. Here we provide a description of cardiac structures and intracardiac events that have been revealed by ultrasonic measurements and angioscopy. Echocardiography revealed that the atrioventricular valves descend deep into the ventricle during ventricular filling and thereby greatly reduce the communication between the systemic (cavum arteriosum) and pulmonary (cavum pulmonale) ventricular chambers during diastole. Angioscopy and echocardiography showed how the two incomplete septa, the muscular ridge and the bulbuslamelle - ventricular structures common to all squamates - contract against each other in systole and provide functional division of the anatomically subdivided ventricle. Washout shunts are inevitable in the subdivided snake ventricle, but we show that the site of shunting, the cavum venosum, is very small throughout the cardiac cycle. It is concluded that the python ventricle is incapable of the pronounced and variable shunts of other snakes, because of its architecture and valvular mechanics.

  18. Separated flow operation of the SHARAQ spectrometer for in-flight proton-decay experiments

    Energy Technology Data Exchange (ETDEWEB)

    Dozono, M., E-mail: dozono@cns.s.u-tokyo.ac.jp [Center for Nuclear Study, University of Tokyo, Saitama 351-0198 (Japan); Uesaka, T. [RIKEN Nishina Center, Saitama 351-0198 (Japan); Michimasa, S.; Takaki, M.; Kobayashi, M.; Matsushita, M.; Ota, S.; Tokieda, H.; Shimoura, S. [Center for Nuclear Study, University of Tokyo, Saitama 351-0198 (Japan)

    2016-09-11

    A new “separated flow” operating mode has been developed for in-flight proton-decay experiments using the SHARAQ spectrometer. In this mode, the protons and heavy-ion products are separated and measured in coincidence at two different focal planes of the SHARAQ. The ion-optical properties of the new mode were studied using a proton beam at 246 MeV, and the momentum vector was reconstructed from the parameters measured in the focal plane of the SHARAQ. In the experiment with the ({sup 16}O, {sup 16}F) reaction at a beam energy of 247 MeV/u, the outgoing {sup 15}O+p produced by the decay of {sup 16}F were measured in coincidence with the SHARAQ. High energy resolutions of 100 keV (FWHM) and ∼2 MeV were achieved for a relative energy of 535 keV and a {sup 16}F kinetic energy of 3940 MeV, respectively. The mode allows a new form of missing-mass spectroscopy using a reaction probe with a particle-decay channel.

  19. Developments in the simulation of separated flows using finite difference methods

    Science.gov (United States)

    Steger, J. L.; Van Dalsem, W. R.

    1985-01-01

    Compressible viscous flow simulation using finite difference Navier-Stokes and viscous-inviscid interaction methods is described. Recent developments are reviewed that significantly improve the computational efficiency of approximately factored implicit Navier-Stokes algorithms. Compared to Navier-Stokes codes, modern viscous-inviscid interaction codes are more computationally efficient, but have restricted application and are more complicated to program. Therefore, less efficient but more general viscous-inviscid interaction methods are investigated that use forcing functions instead of boundary condition matching, and a simple, direct/inverse, three-dimensional, finite-difference, boundary layer code is presented.

  20. Dynamic cross-flow filtration: enhanced continuous small-scale solid-liquid separation.

    Science.gov (United States)

    Gursch, Johannes; Hohl, Roland; Dujmovic, Diana; Brozio, Jörg; Krumme, Markus; Rasenack, Norbert; Khinast, Johannes

    2016-01-01

    In a previous study, a small-scale dynamic filtration device (SFD) was analyzed and the basic mechanisms governing the filtration process were characterized. The present work aims at improving the device's performance in terms of actual production. Various operation modes were tested in order to increase permeate flow and concentration factors (CF), while maintaining a fully continuous production mode. Both, a vacuum-enhanced and a pulsating operation mode, proved to be superior to the currently implemented open-operation mode. For example, for lactose, an increase of the CF could be achieved from 1.7 in open mode to 7.6 in pulsating operation mode. The investigated operation strategy enables process control systems to rapidly react to fluctuating feeds that may occur due to changes in upstream manufacturing steps. As a result, not only filtration performance in terms of permeate rate but also process flexibility can be significantly increased. Overall, vacuum-enhanced operation was shown to be most promising for integration into an industrial environment. The option to elevate achievable concentration factors, ease of flow monitoring as well as the ability to react to changes in the feed conditions allow for effective and efficient continuous small-scale filtration.

  1. An implicit time-marching method for studying unsteady flow with massive separation

    Science.gov (United States)

    Osswald, G. A.; Ghia, K. N.; Chia, U.

    1985-01-01

    A fully implicit time-marching method is developed such that all spatial derivatives are approximated using central differences, but no use is made of any artificial dissipation. The numerical method solves the discretized equations using Alternating Direction Implicit-Block Gaussian Elimination technique. The method is implemented in the unsteady analysis, which solves the incompressible Navier-Stokes equations in terms of vorticity and stream function in generalized orthogonal coordinates. A clustered conformal C-grid is employed, and every effort is made to resolve the various length scales in the flow problem. The metric discontinuity at the branch-cut is treated appropriately using analytic continuation. Introduction of the BGE reordering permits implicit treatment of the branch cut in the numerical method. The vorticity singularity at the cusped trailing edge is also appropriately treated. This accurate and efficient implicit method is used to study flow at Re = 1000, past a 12-percent thick symmetric Joukowski airfoil at high angle of attack 30 and 53 deg.

  2. Continuous thermochromatographic separation of carrier-free radioisotopes of platinum elements in air flow from products of nuclear reactions in beam of heavy-ion accelerator

    Energy Technology Data Exchange (ETDEWEB)

    Domanov, V.P.; Zvara, I.

    1984-01-01

    The thermochromatographic behaviour of volatile oxygen-containing compounds of platinum group metals in on-line regime in an accelerator beam has been studied to evaluate possibilities of chemical separation of the Z=108-110 elements. Volatile compounds of radioplatinoids were prepared while decelerating the recoil atoms - nuclear reaction products - in a flow of dry (partial water vapour pressure below 10/sup -4/ Pa) or humid air (saturated with H/sub 2/O vapours at 0 deg C) and they were extracted on the walls of a quartz thermochromatographic column. Under certain experimental conditions the maximum of /sup 173/OsO/sub 4/ precipitation zone is located at -88 +- 10 deg C, and that for sup(183, 184)IrO/sub 3/ - at 80 +- 10 deg C. It is shown that the fast response of the method for Os is not worse than 1 s. A high volatility of sup(99, 100)Rh has been observed for the first time, being evidently related to the RhO/sub 3/ formation. Ir and /sup 187/Pt in the humid air flow were precipitated at 20-10 deg C. Possible composition of compounds formed is being discussed. Possibility of using, in principle, oxygen-containing compounds for chemical identification of Z=108-110 elements is pointed out.

  3. 新型复合T型管对液液二相流的分离%Phase separation of liquid-liquid two-phase flow by multi-tube T-junction separator

    Institute of Scientific and Technical Information of China (English)

    杨利民; 赵振莹; 沈小明; 赵立立

    2012-01-01

    T-junction separator is a novel type of two-phase flow separator with the advantages of compact, continuous, simple, economical and safe operations as well as being easy for installation, replacement and maintenance, but its separation efficiency of the simple T-junction separator is low for separating the two-phase flow. The multi-tube T-junction separator combines several T-junctions in one unit for improving the separation efficiency of the two-phase flow. With kerosene and water as working fluids, the separation experiments were carried out by using the simple T and the multi-tube T-junction units with main pipe in horizontal and branch in vertically upward under the flow patterns of stratified flow and the stratified flow with mixing at interface. The results show that under these two patterns the separation efficiencies of the two phases for the multi-tube T-junctions are much higher than those of the simple T-junction. Increasing the number of connecting tubes in the multi-tube T-junction unit can improve the separation efficiency. Generally, for the stratified flow,the complete separation of two phases can be achieved by a multi-tube T-junction separator with 5 or more connecting tubes; increasing the mixedness of the inlet flow will cause the drop of the separation efficiency peak; the effect of water volume fraction on the separation efficiency is associated with the flow pattern. The phase separation of liquid-liquid two-phase flow by using the multi-tube T-junction separator is a promising technique for industrial application.%T型管分离器是一类新型的二相流分离器,具有集约、连续、简单、经济、安全以及安装、更新、维护方便等优点,缺点是简单T型管作为分离器在分离二相流时的分离效率往往不高,只能起到部分分离的作用.新型复合T型管是对简单T型管的一种改进,以期能提高二相流的分离效率.以煤油和水为液液二相工作介质,用主管水平,侧支管垂

  4. Aircraft aerodynamic prediction method for V/STOL transition including flow separation

    Science.gov (United States)

    Gilmer, B. R.; Miner, G. A.; Bristow, D. R.

    1983-01-01

    A numerical procedure was developed for the aerodynamic force and moment analysis of V/STOL aircraft operating in the transition regime between hover and conventional forward flight. The trajectories, cross sectional area variations, and mass entrainment rates of the jets are calculated by the Adler-Baron Jet-in-Crossflow Program. The inviscid effects of the interaction between the jets and airframe on the aerodynamic properties are determined by use of the MCAIR 3-D Subsonic properties are determined by use of the MCAIR 3-D Subsonic Potential Flow Program, a surface panel method. In addition, the MCAIR 3-D Geometry influence Coefficient Program is used to calculate a matrix of partial derivatives that represent the rate of change of the inviscid aerodynamic properties with respect to arbitrary changes in the effective wing shape.

  5. Separating xylose from glucose using spiral wound nanofiltration membrane: Effect of cross-flow parameters on sugar rejection

    Science.gov (United States)

    Roli, N. F. M.; Yussof, H. W.; Seman, M. N. A.; Saufi, S. M.; Mohammad, A. W.

    2016-11-01

    A solution model consisted of two different monosaccharides namely xylose and glucose were separated using a pilot scale spiral wound cross-flow system. This system was equipped by a commercial spiral wound nanofiltration (NF) membrane, Desal-5 DK, having a molecular weight cut off (MWCO) of 150-300 g mol-1. The aim of this present work is to investigate the effect of the cross-flow parameters: the trans-membrane pressure (TMP) and the feed concentration (C0) on the xylose separation from glucose. The filtration experiments were carried out in total reflux mode with different feed concentration of 2, 5, and 10 g/L at different TMP of 5,8 and 10 bar. The performances of the NF membrane were evaluated by measuring the permeate flux and sugar rejection for each experiment. All the samples were quantified using a high performance liquid chromatography equipped by a fractive index detector. The experimental results indicated an increase in pressure from 5 to 10 bar which was a notable increase to the permeate fluxes from 2.66 × 10-3 to 4.14 × 10-3L m-2s-1. Meanwhile, an increase in the C0 increases the xylose rejection. At TMP of 10 bar and C0 of 5 g/L, the observed xylose rejection and glucose rejection were measured at 67.19% and 91.82%, respectively. The lower rejection in xylose than glucose suggested that larger glucose molecule were not able to easily pass through the membrane compared to the smaller xylose molecule. The results of this phenomena proved that NF with spiral wound configuration has the potential to separate xylose from glucose, which is valuable to the purification of xylose in xylose production as an alternative to chromatographic processes.

  6. Concentration gradient focusing and separation in a silica nanofluidic channel with a non-uniform electroosmotic flow.

    Science.gov (United States)

    Hsu, Wei-Lun; Harvie, Dalton J E; Davidson, Malcolm R; Jeong, Helen; Goldys, Ewa M; Inglis, David W

    2014-09-21

    The simultaneous concentration gradient focusing and separation of proteins in a silica nanofluidic channel of various geometries is investigated experimentally and theoretically. Previous modelling of a similar device [Inglis et al., Angew. Chem. Int. Ed., 2011, 50, 7546] assumed a uniform velocity profile along the length of the nanochannel. Using detailed numerical analysis incorporating charge regulation and viscoelectric effects, we show that in reality the varying axial electric field and varying electric double layer thickness caused by the concentration gradient, induce a highly non-uniform velocity profile, fundamentally altering the protein trapping mechanism: the direction of the local electroosmotic flow reverses and two local vortices are formed near the centreline of the nanochannel at the low salt concentration end, enhancing trapping efficiency. Simulation results for yellow/red fluorescent protein R-PE concentration enhancement, peak focusing position and peak focusing width are in good agreement with experimental measurements, validating the model. The predicted separation of yellow/red (R-PE) from green (Dyl-Strep) fluorescent proteins mimics that from a previous experiment [Inglis et al., Angew. Chem. Int. Ed., 2011, 50, 7546] conducted in a slightly different geometry. The results will inform the design of new class of matrix-free particle focusing and separation devices.

  7. Flowing atmospheric pressure afterglow combined with laser ablation for direct analysis of compounds separated by thin-layer chromatography.

    Science.gov (United States)

    Cegłowski, Michał; Smoluch, Marek; Reszke, Edward; Silberring, Jerzy; Schroeder, Grzegorz

    2016-01-01

    A thin-layer chromatography-mass spectrometry (TLC-MS) setup for characterization of low molecular weight compounds separated on standard TLC plates has been constructed. This new approach successfully combines TLC separation, laser ablation, and ionization using flowing atmospheric pressure afterglow (FAPA) source. For the laser ablation, a low-priced 445-nm continuous-wave diode laser pointer, with a power of 1 W, was used. The combination of the simple, low-budget laser pointer and the FAPA ion source has made this experimental arrangement broadly available, also for small laboratories. The approach was successfully applied for the characterization of low molecular weight compounds separated on TLC plates, such as a mixture of pyrazole derivatives, alkaloids (nicotine and sparteine), and an extract from a drug tablet consisting of paracetamol, propyphenazone, and caffeine. The laser pointer used was capable of ablating organic compounds without the need of application of any additional substances (matrices, staining, etc.) on the TLC spots. The detection limit of the proposed method was estimated to be 35 ng/cm(2) of a pyrazole derivative.

  8. Simulation of Multiphase Flow of the Oil-Water Separation in a Rotating Packed Bed for Oil Purification

    Directory of Open Access Journals (Sweden)

    Xiaojun Zhang

    2013-01-01

    particle contaminant, moisture and gas simultaneously. As the major unit of HIGEE, the RPB uses centrifugal force to intensify mass transfer. Because of the special structure of RPB, the hydraulic characteristics of the RPB are very important. In this study, the multiphase flow model in porous media of the RPB is presented, and the dynamical oil-water separation in the RPB is simulated using a commercial computational fluid dynamics code. The operating conditions and configuration on the hydraulic performance of the RPB are investigated. The results have indicated that the separation efficiency of HIGEE rotating oil purifier is predominantly affected by operating conditions and the configurations. The best inlet pressure is 0.002 MPa. When the liquid inlet is placed in the outside of the lower surface of RPB; oil outlet is placed in the upper surface, where it is near the rotation axis; and water outlet is placed in the middle of the RPB, where it is far away from the oil outlet, the separating efficiency is the best.

  9. Size separations of starch of different botanical origin studied by asymmetrical-flow field-flow fractionation and multiangle light scattering.

    Science.gov (United States)

    Wahlund, Karl-Gustav; Leeman, Mats; Santacruz, Stalin

    2011-02-01

    Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 10(6) g mol(-1), and most of the second size population had molar masses above 10(7) g mol(-1). Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties.

  10. A Scenario Based Approach to Separate the Impacts of Land Use and Climate Alteration on Daily Flow Regime Indices

    Science.gov (United States)

    Darabi, Hamid; Torabi Haghighi, Ali; Fazel, Nasim; Klöve, Björn

    2017-04-01

    Land use and climate changes have important impacts on water resources such as river flow regimes and they are often complicated to separate at the watershed scale. To separate impact, we develop a scenario based approach using remote sensing and hydro-climatological data. Using the framework, we assess the on hydrological indices in Marboreh watershed (headwater of Dez River which modified by the most important hydropower plant in Iran). The analysis is based on data from three Landsat TM images (1988, 1998 and 2008), meteorological data (1983-2012) at Aligudarz station and hydrological data (1983-2012) at Doroud gauge station. To carry out the study, the QUAC module and supervised classification (ML algorithm) in the ENVI 5.1, the SWAT model and Mann-Kendall method were used for remote sensing, hydrological modeling and trend analysis respectively. To analyses the impact of land use and climate changes, the studied period was divided into three decades (1983-1992, 1993-2002 and 2003-2012). For all periods, the land use maps were assigned based on the middle year of each decade (1988, 1998 and 2008). Then, 10 hydrological indices related to high flow and low flow indices (HDI and LDI) were analyzed for seven scenarios which were created by combining predefined climatic periods and land use maps. Base on the RS analysis, the major alterations in land use including degradation of natural rangeland (-18.49%) and increasing farming land (+16.70%) and residential area (+0.80%) were assessed from 1988 to 2008. The Mann-Kendall test indicates a statistically decreasing trend in rainfall induced runoff and increasing trend in the temperature at the 5% and 1% significance levels, respectively. The results of this study clearly showed that in Marboreh watershed is influenced by climate variability impact on hydrological indices more than land use change. Also, the present study demonstrated that the low flow indices were affected more than high flow indices in both climate

  11. Characterization of the physical parameters in a process of magnetic separation and pressure-driven flow of a magnetic fluid

    Science.gov (United States)

    Cunha, F. R.; Sobral, Y. D.

    2004-11-01

    The equations governing the motion of a magnetic fluid are presented. These equations are non-linear and give rise to non-Newtonian effects attributable to the magnetization of the fluid. The equations are made dimensionless and the physical parameters of the coupled hydrodynamic-magnetic problem identified. The study is first applied to describe the motion of a magnetic droplet freely suspended in a viscous fluid undergoing a permanent magnetic field. A first-order theory is developed for the regime of small drop deformation in which viscous forces dominate inertial hydrodynamic force. At this regime, it is shown that the drift velocity of a magnetic drop scales with the square of the applied magnetic field and the deformation of the drop scales linearly with the applied field. Experiments are carried out and the range of validity of the small deformation analysis determined. The pressure-driven flow of a magnetic fluid is solved by a regular asymptotic expansion for two cases: a Poiseuille flow of a single magnetic fluid and a core pipe flow with a magnetic fluid adjacent to the tube wall. The theory is used to predict the volume rate of a viscous magnetic fluid separated from a non-magnetic viscous fluid by the action of a magnetic field. The apparent viscosity of a magnetic fluid as a function of magnetic parameters is also examined from our theory. A possible application of the present theoretical studies is on the remediation technology addressed to oil spills in natural environments.

  12. Visualization of Flow Separation Around an Atmospheric Entry Capsule at Low-Subsonic Mach Number Using Background-Oriented Schlieren (BOS)

    Science.gov (United States)

    Mizukaki, Toshiharu; Borg, Stephen E.; Danehy, Paul M.; Murman, Scott M.

    2014-01-01

    This paper presents the results of visualization of separated flow around a generic entry capsule that resembles the Apollo Command Module (CM) and the Orion Multi-Purpose Crew Vehicle (MPCV). The model was tested at flow speeds up to Mach 0.4 at a single angle of attack of 28 degrees. For manned spacecraft using capsule-shaped vehicles, certain flight operations such as emergency abort maneuvers soon after launch and flight just prior to parachute deployment during the final stages of entry, the command module may fly at low Mach number. Under these flow conditions, the separated flow generated from the heat-shield surface on both windward and leeward sides of the capsule dominates the wake flow downstream of the capsule. In this paper, flow visualization of the separated flow was conducted using the background-oriented schlieren (BOS) method, which has the capability of visualizing significantly separated wake flows without the particle seeding required by other techniques. Experimental results herein show that BOS has detection capability of density changes on the order of 10(sup-5).

  13. Mathematical model of the two-phase flow in a vertical well with an electric centrifugal pump located in the permafrost region

    Science.gov (United States)

    Musakaev, N. G.; Borodin, S. L.

    2016-05-01

    The mathematical model of the two-phase flow in a vertical well with an electric centrifugal pump located in the permafrost region is presented. The comparison of the calculation's results with experimental data, the results of numerical experiments by determining the flow structure, the temperature distribution in a well, influence of the temperature distribution on paraffin deposition and change in time of the radius of thawing in the frozen ground are presented.

  14. Effect of synthetic jets physical parameters on flow separation control over airfoil%合成射流物理参数对控制翼型流动分离的影响

    Institute of Scientific and Technical Information of China (English)

    刘峰; 邹建锋; 郑耀

    2013-01-01

    The effect of synthetic jets on controlling flow separation over a NACA0015 airfoil was numerically analyzed using computational fluid dynamic (CFD) method. The synthetic jets were located at 12%c, 30%c and 70%c, respectively, from the leading edge (c is the chord length of the airfoil). The trends of flow separation control effect at these locations with the variation of angle of attack (AOA) and jet angle were analyzed. Meanwhile, the locations, phase angle and momentum coefficient of multi-location synthetic jets were analyzed. The two-dimensional unsteady incompressible Navier-Stokes equations were used to simulate unsteady separated flows. SST turbulence model was utilized for the computation, and the pressure-implicit with splitting of operators (PISO) algorithm was utilized for pressure correction. An implicit numerical treatment was adopted for time integral and the second order upwind scheme for space discretization. The numerical results show that: 1) when synthetic jets are located at 12%c, 30%c and 70%c from the leading edge, the tangential jet shows better control effect than normal jet; 2) for single and multi-location synthetic jets, the jet locations should be close to the separation point or prior to the separation point, only in this occasion can the purpose of flow control be achieved, and the closer the jet locations to the separation point, the better control effect can be expected; 3) the performance of multi-location synthetic jets can be improved by changing phase angle of multi-location synthetic jets.%应用计算流体力学(CFD)技术数值模拟了合成射流对NACA0015翼型流动控制的影响.合成射流施加的位置分别距离翼型前缘12%c、30%c和70%c(c为翼型的弦长),研究分析在不同位置施加合成射流,控制流动分离的效果随攻角和射流偏角的变化趋势,对组合射流的位置、相位角和动量系数进行研究.以二维不可压非定常Reynolds-averaged Navier-Stokes(RANS)方程

  15. The use of immunomagnetic separation of erythrocytes in the in vivo flow cytometer-based micronucleus assay.

    Science.gov (United States)

    Abramsson-Zetterberg, Lilianne; Carlsson, Rickard; Sand, Salomon

    2013-04-15

    The use of sensitive test systems makes it possible to detect weakly genotoxic chemicals and to better define the shape of dose-response relationships, which make it easier to interpret the mechanism behind possible effects. In this study we have refined the flow cytometer-based micronucleus assay by use of a cytometer equipped with two lasers. Since micronucleated young polychromatic erythrocytes, MNPCE, are very few in number among the cells in peripheral blood, about one or two out of 100,000 erythrocytes, there is always a risk that other cells, doublets or crystals, by mistake will be classified as a MNPCE. With immunomagnetic separation of the very youngest erythrocytes - which are transferrin-positive (Trf+Ret) - prior to analysis, we have obtained an almost pure (>98%) Trf+Ret-population. To clarify whether this separation of cells prior to analysis increases the sensitivity of the already sensitive and further refined flow cytometer-based micronucleus assay, we studied the dose-response towards benzo(a)pyrene, B[a]P in the low-dose region, 0-30mg/kgbw. Thirty FVB mice were intraperitoneally injected with B[a]P. From the same blood samples collected from these mice, cells were prepared in the two different ways and analyzed in the flow-cytometer equipped with two lasers. The lowest dose of B[a]P that can be reliably determined without being overwhelmed by the estimated error was about the same for the two methods, about 7mg/kgbw, i.e. the immunomagnetic separation did not increase the sensitivity. A second study with BalbC mice strengthens the result obtained with the FVB mice. Prior to the low-dose study the optimal sampling time for the two methods was determined. In this case, the water-solouble chemical acrylamide was used. The time courses obtained show almost the same shape of the curves, with a maximum of fMNPCE and fMNTrf+Ret at about 40-50h after exposure.

  16. Separate process wastewaters, part A: Contaminated flow collection and treatment system for the Kansas City Plant

    Energy Technology Data Exchange (ETDEWEB)

    1995-01-01

    The U.S. Department of Energy (DOE) has prepared this Environmental Assessment (EA) to assist the agency in complying with the National Environmental Policy Act (NEPA) of 1969 as it applies to modification of ongoing groundwater treatment at DOE`s Kansas City Plant (KCP), located about 19 km (12 miles) south of the central business district of Kansas City, Missouri. The KCP is currently owned by DOE and is operated by the Kansas City Division of AlliedSignal Inc. The plant manufactures nonnuclear components for nuclear weapons. The purpose of and need for the DOE action is to treat identified toxic organic contaminated groundwater at the KCP to ensure that human health and the environment are protected and to comply with groundwater treatment requirements of the U.S. Environmental Protection Agency (EPA) Resource Conservation and Recovery Act (RCRA) 3008(h) Administrative Order on Consent and the discharge requirements of the Kansas City, Missouri, ordinances for the city sewer system. Four source streams of toxic organic contaminated groundwater have been identified that require treatment prior to discharge to the city sewer system. The toxic organic contaminants of concern consist of volatile organic compounds (VOCS) in the groundwater and polychlorinated biphenyls (PCBS) predominantly associated with some soils near the Main Manufacturing Building. The no-action alternative is to continue with the current combination of treatment and nontreatment and to continue operation of the KCP groundwater treatment system in its current configuration at Building 97 (B97). The DOE proposed action is to collect and treat all identified toxic organic contaminated groundwater prior to discharge to the city sewer system. The proposed action includes constructing an Organics Collection System and Organics Treatment Building, moving and expanding the existing groundwater treatment system, and operating the new groundwater treatment facility.

  17. Experimental evaluation of the effect of a modified port-location mode on the performance of a three-zone simulated moving-bed process for the separation of valine and isoleucine.

    Science.gov (United States)

    Park, Chanhun; Nam, Hee-Geun; Kim, Pung-Ho; Mun, Sungyong

    2014-06-01

    The removal of isoleucine from valine has been a key issue in the stage of valine crystallization, which is the final step in the valine production process in industry. To address this issue, a three-zone simulated moving-bed (SMB) process for the separation of valine and isoleucine has been developed previously. However, the previous process, which was based on a classical port-location mode, had some limitations in throughput and valine product concentration. In this study, a three-zone SMB process based on a modified port-location mode was applied to the separation of valine and isoleucine for the purpose of making a marked improvement in throughput and valine product concentration. Computer simulations and a lab-scale process experiment showed that the modified three-zone SMB for valine separation led to >65% higher throughput and >160% higher valine concentration compared to the previous three-zone SMB for the same separation.

  18. Measurement of global and separate renal blood flow with cinetomodensitometry; Mesure du debit sanguin renal global et separe par la cine-tomodensitometrie

    Energy Technology Data Exchange (ETDEWEB)

    Froissart, M.; Archambaud, F.; Hernigou, A.; Prigent, A.; Paillard, M. [Hopital Broussais, 75 - Paris (France)

    1994-12-01

    EBCT flow study offers a promising new approach to measure the renal blood flow. In vitro and in vivo studies were performed to resolve methodological problems such as checking the linear relation between contrast concentration and Hounsfield units or determination of the Threshold used for the mapping. First measurements of renal volumes and flow performed in patients showed expected values. (authors). 55 refs., 1 tab.

  19. Mechanical coupling between two innovative theories on erosion, transportation and phase-separation: Solving some long-standing problems in mass flows

    Science.gov (United States)

    Pudasaini, Shiva P.; Fischer, Jan-Thomas; Mergili, Martin

    2017-04-01

    Debris flows are gravity driven mixture flows of soil, sand, rock and water. The solid particles and viscous fluid governs the rheological properties, and their coupling significantly influences the dynamics. For example, debris flows can dramatically increase their volume and destructive potential, and become exceptionally mobile by entraining bed material. The mixture composition can evolve to strikingly change the spatial distribution of particles and fluid, and thus frictional and viscous resistance. So, erosion-deposition and phase-separation between solid and fluid, which strongly depend on material composition, play a critical role in debris flow dynamics. Proper understanding of these complex physical processes is very important in accurate description of impact forces, inundation areas, landscape evolution and developing reliable mitigation plans. Predicting the underlying processes of erosion, phase-separation and deposition in debris flow are long-standing challenges. However, due to lack of data and suitable models, there exists no runout prediction method that includes observed processes of erosion of dry and saturated beds, entrainment and diffusion of eroded material, grain sorting, phase-separation, levee/lobe formation and evolution of deposition patterns. Based on innovative mechanical models for erosion-deposition (Pudasaini and Fischer, 2016a) and phase-separation (Pudasaini and Fischer, 2016b) that explicitly consider changes in local flow compositions, and their basic/potential validations, we present a novel, unified, efficient and fully coupled solution method to these true multi-phase, three-dimensional mass flow problems. As debris flows are better described by a three-phase mixture that include viscous fluid, and fine and coarse grains as compared to often used single-phase models, we propose model extensions that consists of three-phases including yield strength. Thus, we present an advanced mass flow simulation model aiming to

  20. Evaluation of Tangential Flow Filtration for the Concentration and Separation of Bacteria and Viruses in Contrasting Marine Environments.

    Science.gov (United States)

    Cai, Lanlan; Yang, Yunlan; Jiao, Nianzhi; Zhang, Rui

    2015-01-01

    Tangential flow filtration (TFF), which has been widely adopted to concentrate a diverse array of microbes from water, is a promising method of microbial separation or removal. However, it is essential to select an optimal membrane suitable for the specific filtration application. This study evaluated two different scales of TFF systems for concentrating and separating microbes (including bacteria and viruses) from contrasting marine waters. Among bacteria-size membranes, polyvinylidene difluoride (PVDF) membranes showed higher bacterial recovery, but lower viral permeation efficiencies than polyethersulfone (PES) membranes, regardless of environments and scales of TFF. Estuary samples showed significantly higher percentages of bacterial retention than nearshore and ocean samples. For virus-size membranes, a higher viral recovery and lower sorption was observed for regenerated cellulose membrane than PES membranes in the small-scale TFF. Similar viral recoveries were observed between PES membranes in the large-scale TFF, with higher viral concentrations being observed in estuary samples than in nearshore samples. Deep ocean samples showed the lowest recovery of viruses, which was consistent with observations of bacterial recovery. Synthetically, PVDF may be more suitable for the concentration of bacterial cells, while PES would be a better choice for the collection of viruses. When compared with the PES membrane, regenerated cellulose is better for viral concentration, while PES is recommended to obtain bacteria- and virus-free seawater.

  1. Effect of boundary representation on viscous, separated flows in a discontinuous-Galerkin Navier-Stokes solver

    Science.gov (United States)

    Nelson, Daniel A.; Jacobs, Gustaaf B.; Kopriva, David A.

    2016-08-01

    The effect of curved-boundary representation on the physics of the separated flow over a NACA 65(1)-412 airfoil is thoroughly investigated. A method is presented to approximate curved boundaries with a high-order discontinuous-Galerkin spectral element method for the solution of the Navier-Stokes equations. Multiblock quadrilateral element meshes are constructed with the grid generation software GridPro. The boundary of a NACA 65(1)-412 airfoil, defined by a cubic natural spline, is piecewise-approximated by isoparametric polynomial interpolants that represent the edges of boundary-fitted elements. Direct numerical simulation of the airfoil is performed on a coarse mesh and fine mesh with polynomial orders ranging from four to twelve. The accuracy of the curve fitting is investigated by comparing the flows computed on curved-sided meshes with those given by straight-sided meshes. Straight-sided meshes yield irregular wakes, whereas curved-sided meshes produce a regular Karman street wake. Straight-sided meshes also produce lower lift and higher viscous drag as compared with curved-sided meshes. When the mesh is refined by reducing the sizes of the elements, the lift decrease and viscous drag increase are less pronounced. The differences in the aerodynamic performance between the straight-sided meshes and the curved-sided meshes are concluded to be the result of artificial surface roughness introduced by the piecewise-linear boundary approximation provided by the straight-sided meshes.

  2. Boundary Conditions and SGS Models for LES of Wall-Bounded Separated Flows: An Application to Engine-Like Geometries

    Directory of Open Access Journals (Sweden)

    Piscaglia F.

    2013-11-01

    Full Text Available The implementation and the combination of advanced boundary conditions and subgrid scale models for Large Eddy Simulations are presented. The goal is to perform reliable cold flow LES simulations in complex geometries, such as in the cylinders of internal combustion engines. The implementation of an inlet boundary condition for synthetic turbulence generation and of two subgrid scale models, the local Dynamic Smagorinsky and the Wall-Adapting Local Eddy-viscosity SGS model ( WALE is described. The WALE model is based on the square of the velocity gradient tensor and it accounts for the effects of both the strain and the rotation rate of the smallest resolved turbulent fluctuations and it recovers the proper y3 near-wall scaling for the eddy viscosity without requiring dynamic pressure; hence, it is supposed to be a very reliable model for ICE simulation. Model validation has been performed separately on two steady state flow benches: a backward facing step geometry and a simple IC engine geometry with one axed central valve. A discussion on the completeness of the LES simulation (i.e. LES simulation quality is given.

  3. Flow injection conductometric system with gas diffusion separation for the determination of Kjeldahl nitrogen in milk and chicken meat.

    Science.gov (United States)

    Junsomboon, Jaroon; Jakmunee, Jaroon

    2008-10-10

    A simple flow injection (FI) conductometric system with gas diffusion separation was developed for the determination of Kjeldahl nitrogen (or proteins) in milk and chicken meat. The sample was digested according to the Kjeldahl standard method and the digest was diluted and directly injected into the donor stream consisting of 4M NaOH. In alkaline medium, ammonium was converted to ammonia, which diffused through the PTFE membrane to dissolve in an acceptor stream (water). Dissociation of ammonia caused a change in conductance of the acceptor solution, which was linearly proportional to the concentration of ammonium originally present in the injected solution. A conductometric flow through cell and an amplifier circuit was fabricated, which helped improve sensitivity of the conductometric detection system. With using a plumbing Teflon tape as a gas diffusion membrane and without thermostating control of the system, a linear calibration graph in range of 10-100mgL(-1) N-NH(4) was obtained, with detection limit of 1mgL(-1) and good precision (relative standard deviation of 0.3% for 11 replicate injections of 50mgL(-1) N-NH(4)). The developed method was validated by the standard Kjeldahl distillation/titration method for the analysis of milk and chicken meat samples. The proposed system had sample throughput of 35h(-1) and consumed much smaller amounts of chemical than the standard method (275mg vs 17.5g of NaOH per analysis, respectively).

  4. Emplacement history and inflation evidence of a long basaltic lava flow located in Southern Payenia Volcanic Province, Argentina

    Science.gov (United States)

    Bernardi, Mauro I.; Bertotto, Gustavo W.; Jalowitzki, Tiago L. R.; Orihashi, Yuji; Ponce, Alexis D.

    2015-02-01

    The El Corcovo lava flow, from the Huanul shield volcano in the southern Mendoza province (central-western Argentina) traveled a distance of 70 km and covered a minimum area of ~ 415 km2. The flow emplacement was controlled both by extrinsic (e.g., topography) and intrinsic (e.g., lava supply rate, lava physicochemical characteristics) factors. The distal portion of the lava flow reached the Colorado River Valley, in La Pampa Province, where it spread and then was confined by earlier river channels. Cross-sections through the flow surveyed at several localities show two vesicular layers surrounding a dense central section, where vesicles are absent or clustered in sheet-shaped and cylindrical-shaped structures. Lavas of the El Corcovo flow are alkaline basalts with low values of viscosity. The morphological and structural characteristics of the flow and the presence of landforms associated with lava accumulation are the evidence of inflation. This process involved the formation of a tabular sheet flow up to 4 m of thick with a large areal extent in the proximal sectors, while at terminal sectors frontal lobes reached inflation values up to 10 m. The numerous swelling structures present at these portions of the flow suggest the movement of lava in lava tubes. We propose that this aspect and the low viscosity of the lava allowed the flow travel to a great distance on a gentle slope relief.

  5. Genesis and Characteristics of Debris Flow Ocurred in 2013 in the Atenquique Ravine, Located on the Eastern Slope of the Colima Volcanic Complex, Mexico.

    Science.gov (United States)

    Suarez-Plascencia, C.; Flores-Pena, S.; Nunez-Cornu, F. J.; Arreola-Ochoa, L. C.; Suarez-Gonzalez, B. V.

    2014-12-01

    Hurricane Manuel affected the Pacific coast of Mexico on September 15 and 16, 2013 causing heavy rainfall of about 240 mm in a 24 hour period in the area of the Volcanic Complex (VC). Heavy rainfall led to the beginning of a significant flow of mud and rocks draining from the Atenquique Creek, located on the eastern slope of the VC in a west east direction. The result of this flow was the heavy damage sustained by the local paper plant located next to the town of Atenquique in the distal part of the basin where the stream is gathered by the Tuxpan River. Damages totaling over 15 million dollars affected a large part in their recycled fibers factory, resulting in an 18-month full stoppage of the factory. This in turn caused a heavy setback of the economy located within a large region of the southern state of Jalisco. Once again on November 25, debris flow occurred only at a lower volume than the September rains, without causing any damage. Both flows contained a viscous and solid liquid flow that left deposits of silt-sandy clasts and other abundant materials of reverse gradation. The first flow reached a thickness of 4.5 m in the Tuxpan riverbed over a length of about 15 km, while the November flow left behind 1.3 m of fine materials and few clasts. The Atenquique ravine historically has had debris flow caused by heavy rainfall from hurricanes. On October 1955 debris flow claimed many deaths and heavy damage to the town and local paper mill. These flows are generated in the summer and they are associated to several factors such as weather, steep slopes, unstable volcanic strata, these elements add an important environmental history in the area, as is the use of continuous deforestation. The current land use has resulted in a positive change from forest to intensive agriculture; but having constant wildfires on the high slopes of the VC and the combination of many other factors such as changes on the soil of the slopes and movement of geological material "scarps and

  6. Characterization of groundwater flow and transport in the General Separations Area, Savannah River Plant: Effect of groundwater withdrawals on the Tuscaloosa-Congaree aquifer head reversal in H Area. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Spalding, C.P.; Duffield, G.M.; Shaw, S.T. [GeoTrans, Inc., Herndon, VA (United States)

    1988-01-01

    The Savannah River Plant (SRP) has maintained a number of sites used for land disposal of various waste materials. The General Separations Area at SRP, located between the Upper Three Runs and Four Mile Creeks, has served as an active area for waste storage for about thirty years. The Tuscaloosa aquifer, which lies beneath the General Separations Area, is a water source for SRP and the surrounding area. The isolation of the Tuscaloosa aquifer has been maintained by an upward hydraulic gradient from the Tuscaloosa aquifer to the overlying Congaree aquifer. This upward gradient is referred to as a hydraulic head reversal in the General Separations Area, i.e., hydraulic heads in the upper Tuscaloosa are higher than hydraulic heads in the Congaree. This head reversal has declined in recent years due to increased groundwater pumping in the upper and lower Tuscaloosa formations. The objective of this investigation is to assess the effects of pumping within the General Separations Area on the Congaree/upper Tuscaloosa head reversal. Methods of maintaining future Tuscaloosa aquifer isolation through the optimization of groundwater withdrawal location and rate were studied. Steady-state and transient groundwater flow models were used to characterize past and potential future groundwater conditions. Future groundwater conditions were simulated for a variety of pumping scenarios.

  7. A State-of-the-Art Review of the Sensor Location, Flow Observability, Estimation, and Prediction Problems in Traffic Networks

    Directory of Open Access Journals (Sweden)

    Enrique Castillo

    2015-01-01

    Full Text Available A state-of-the-art review of flow observability, estimation, and prediction problems in traffic networks is performed. Since mathematical optimization provides a general framework for all of them, an integrated approach is used to perform the analysis of these problems and consider them as different optimization problems whose data, variables, constraints, and objective functions are the main elements that characterize the problems proposed by different authors. For example, counted, scanned or “a priori” data are the most common data sources; conservation laws, flow nonnegativity, link capacity, flow definition, observation, flow propagation, and specific model requirements form the most common constraints; and least squares, likelihood, possible relative error, mean absolute relative error, and so forth constitute the bases for the objective functions or metrics. The high number of possible combinations of these elements justifies the existence of a wide collection of methods for analyzing static and dynamic situations.

  8. THE WAY OF ORGANIZING THE FLOW OF AIRCRAFT BY INVERSE "TROMBONE" TYPE LOCATION OF THE USEFUL MANOEUVRING AREA

    Directory of Open Access Journals (Sweden)

    G. N. Lebedev

    2015-01-01

    Full Text Available The problem of solving important practical tasks of organizing the flow of arriving aircraft from different directions to the same navigation point is described. This allows us to form list for landing and determine their priority.

  9. Local mechanisms for the separation of optic flow-field components in the land crab, Cardisoma guanhumi: a role for motion parallax?

    Science.gov (United States)

    Johnson, Aaron P; Barnes, W Jon P; Macauley, Martin W S

    2004-01-01

    Although a number of global mechanisms have been proposed over the years that explain how crabs might separate the rotational and translational components of their optic flow field, there has been no evidence to date that local mechanisms such as motion parallax are used in this separation. We describe here a study that takes advantage of a recently developed suite of computer-generated visual stimuli that creates a three-dimensional world surrounding the crab in which we can simulate translational and rotational optic flow. We show that, while motion parallax is not the only mechanism used in flow-field separation, it does play a role in the recognition of translational optic flow fields in that, under conditions of low overall light intensity and low contrast ratio when crabs find the distinction between rotation and translation harder, smaller eye movements occur in response to translation when motion parallax cues are present than when they are absent. Thus, motion parallax is one of many cues that crabs use to separate rotational and translational optic flow by showing compensatory eye movements to only the former.

  10. Enhancement of continuous-flow separation of viable/nonviable yeast cells using a nonuniform alternating current electric field with complex spatial distribution.

    Science.gov (United States)

    Tada, Shigeru; Nakanishi, Arisa; Eguchi, Masanori; Ochi, Kengo; Baba, Megumi; Tsukamoto, Akira

    2016-05-01

    The variability in cell response to AC electric fields is selective enough to separate not only the cell types but also the activation states of similar cells. In this work, we use dielectrophoresis (DEP), which exploits the differences in the dielectric properties of cells, to separate nonviable and viable cells. A parallel-plate DEP device consisting of a bottom face with an array of micro-fabricated interdigitated electrodes and a top face with a plane electrode was proposed to facilitate the separation of cells by creating a nonuniform electric field throughout the flow channel. The operation and performance of the device were evaluated using live and dead yeast cells as model biological particles. Further, numerical simulations were conducted for the cell suspensions flowing in a channel with a nonuniform AC electric field, modeled on the basis of the equation of motion of particles, to characterize the separation efficiency by changing the frequency of applied AC voltage. Results demonstrated that dead cells traveling through the channel were focused onto a site around the minimum electric field gradient in the middle of the flow stream, while live cells were trapped on the bottom face. Cells were thus successfully separated under the appropriately tuned frequency of 1 MHz. Predictions showed good agreement with the observation. The proposed DEP device provides a new approach to, for instance, hematological analysis or the separation of different cancer cells for application in circulating tumor cell identification.

  11. Gas-Flow Switch Recovery Experiments

    Science.gov (United States)

    1983-06-01

    of laminated Plexiglas. The upper wall of the spark gap flow channel could be pivoted to adjust the minimum separation of the nozzle, which will... flow channel. PiVOT " Fig. 3. Fig. 4. Cross section of spark gap flow channel showing location of electrodes and pressure orifices. r-------~5~cm...flow in the spark gap . Flow conditions were essentially determined by the choice of air supply pressure in the wind tunnel plenum which was located

  12. Locations, values, and uncertainties of hydraulic-head observations for the transient ground-water flow model, Death Valley regional ground-water flow system, Nevada and California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This digital geospatial data set contains the locations, values, and uncertainties of hydraulic-head observations used in the calibration of the transient model of...

  13. Locations, values, and uncertainties of hydraulic-head observations for the transient ground-water flow model, Death Valley regional ground-water flow system, Nevada and California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This digital geospatial data set contains the locations, values, and uncertainties of hydraulic-head observations used in the calibration of the transient model of...

  14. Computational studies of reacting flows with applications to zinc selenide nanoparticle synthesis and methane/hydrogen separation

    Science.gov (United States)

    Koutsona, Maria

    a predictive model describing pressure and concentration dynamics during Pressure Swing Adsorption (PSA) of binary (or pseudo-binary) gas mixtures. The separation of metane-hydrogen mixtures over 5A-zeolite was used as an example. The PSA cycle considered in this study includes the following 5 steps: (1) pressurization with product, (2) high-pressure adsorption, (3) cocurrent depressurization, (4) countercurrent blowdown and (5) countercurrent purge with product at low pressure. The PSA mathematical model describes the following processes gas flow in the bed (as axially dispersed plug flow) and the mass balance of the components of the mixture coupled to adsorption/desorption kinetics. The model results in a system of coupled partial differential equations in the axial bed dimension and time. The Galerkin Finite Element Method was used to discretize the equations in the axial direction of the bed. The resulting system of ordinary differential equations (ODE's) in time is solved by using an Euler full-implicit scheme. The model is being used by Chemical Design, Inc., for the initial design of PSA units.

  15. Effect of surface permeability on the structure of a separated turbulent flow and heat transfer behind a backward-facing step

    Science.gov (United States)

    Terekhov, V. V.; Terekhov, V. I.

    2017-03-01

    The structure and heat transfer in a turbulent separated flow in a suddenly expanding channel with injection (suction) through a porous wall are numerically simulated with the use of two-dimensional averaged Navier-Stokes equations, energy equations, and v 2- f turbulence model. It is shown that enhancement of the intensity of the transverse mass flux on the wall reduces the separation region length in the case of suction and increases the separation region length in the case of injection up to complete boundary layer displacement. The maximum heat transfer coefficient as a function of permeability is accurately described by the asymptotic theory of a turbulent boundary layer.

  16. Effects of light intensity and pattern contrast on the ability of the land crab, Cardisoma guanhumi, to separate optic flow-field components.

    Science.gov (United States)

    Johnson, Aaron P; Barnes, W Jon P; Macauley, Martin W S

    2004-01-01

    Using a novel suite of computer-generated visual stimuli that mimicked components of optic flow, the visual responses of the tropical land crab, Cardisoma guanhumi, were investigated. We show that crabs are normally successful in distinguishing the rotational and translational components of the optic flow field, showing strong optokinetic responses to the former but not the latter. This ability was not dependent on the orientation of the crab, occurring both in "forwards-walking" and "sideways-walking" configurations. However, under conditions of low overall light intensity and/or low object/background contrast, the separation mechanism shows partial failure causing the crab to generate compensatory eye movements to translation, particularly in response to low-frequency (low-velocity) stimuli. Using this discovery, we then tested the ability of crabs to separate rotational and translational components in a combined rotation/translation flow field under different conditions. We demonstrate that, while crabs can successfully separate such a combined flow field under normal circumstances, showing compensatory eye movements only to the rotational component, they are unable to make this separation under conditions of low overall light intensity and low object/background contrast. Here, the responses to both flow-field components show summation when they are in phase, but, surprisingly, there is little reduction in the amplitude of responses to rotation when the translational component is in antiphase. Our results demonstrate that the crab's visual system finds separation of flow-field components a harder task than detection of movement, since the former shows partial failure at light intensities and/or object/background contrasts at which movement of the world around the crab is still generating high-gain optokinetic responses.

  17. 文丘里分离罐气相流场特性及分离效率研究%Characteristics of Gas Flow Field and Separation Efficiency of Two Venturi Separators with Different Structure

    Institute of Scientific and Technical Information of China (English)

    陈雪莉; 李昌剑; 刘万洲; 刘旭军; 罗永恒; 杨红波; 于飞; 杨丽坤; 王俭

    2011-01-01

    采用雷诺应力湍流模型和离散颗粒模型对2种不同结构文丘里分离罐内气相和颗粒相进行了数值模拟,对比了其气相流场特性和分离效率.结果表明,侧出口分离罐A内的切向速度分布对称性较差,外旋流区较小,对分离不利;上出口分离罐B内的切向速度分布呈兰金涡分布,对称性良好,外旋流区较大,适合分离.2分离罐对粒径小于5μm颗粒的分离效率比较接近;粒径5μm~45μm的颗粒,B罐的分离效率明显高于A罐;粒径大于45μm的颗粒,A罐的分离效率略高于B罐.%Reynolds stress model (RSM) and Discrete particle model (DPM) were used to respectively simulate the gas phase and particle phase in two venturi separators with different structures, meanwhile, the flow characteristics and separation efficiency were analyzed and compared. The results showed that the distribution of tangential velocity in the venturi separator with a side outlet (A) takes the shape of "twist of dough", and appears distinctly asymmetrical, while the one with a upward outlet (B) takes the shape of "Rankine vortex", and appears good symmetrical. Compared with venturi separator B, the area of outer swirling-flow of separator A is smaller, which is not in favor of separation. Two separators have the similar efficiencies for the particles less than 5|im. For the particles between 5μm and 45μm, the efficiencies of separator B are higher than those of separator A. However, the efficiencies of separator A are slightly higher for the particles above 45|xm. The results provide a theoretical basis for the structure optimization of the venturi separator.

  18. 新型轴流导叶式气液旋流器流场数值模拟%Numerical Simulation of the Flow Filed of Axial-flow Vane-Guided Cyclone for Gas-Liquid Separation

    Institute of Scientific and Technical Information of China (English)

    黄龙; 邓松圣; 王斌; 陈志

    2015-01-01

    A numerical simulation of the flow filed of axial⁃flow vane⁃guided cyclone for gas⁃liquid separation was carried out using CFD software. Hybrid grid on split volumes was used to improve the quality of the grid and the turbulence simulation was based on RSM model. The results include velocity and pressure field of the cyclone, and show that they are different from the traditional cyclone’ s. The flow field is of good symmetrical, and there exists only downward external vortex and no upward inner vortex in the straight part. The tangential velocity and axial velocity distribution is the same but radial velocity is very small. The pressure drop is mainly caused by dynamic pressure loss, and the biggest loss is located in the flow around the vanes.%针对某新型轴流导叶式气液旋流器,利用CFD软件进行了真实工况下的流场数值模拟。采用分区段的混合网格提高了网格质量,湍流模拟采用了精度较高的RSM模型。通过模拟得到了旋流器内部流场的速度及压强变化规律,结果表明:该旋流器流场与传统旋流器有较大区别,流场对称性很好,且直筒部分流场只存在下降外旋流,而无上升内旋流;切向速度与轴向速度分布规律相似,径向速度由于直筒结构保持较小水平;压降主要表现为动压损失,且导叶处压降损失最大。

  19. A Mathematical Model of Membrane Gas Separation with Energy Transfer by Molecules of Gas Flowing in a Channel to Molecules Penetrating this Channel from the Adjacent Channel

    Directory of Open Access Journals (Sweden)

    Szwast Maciej

    2015-06-01

    Full Text Available The paper presents the mathematical modelling of selected isothermal separation processes of gaseous mixtures, taking place in plants using membranes, in particular nonporous polymer membranes. The modelling concerns membrane modules consisting of two channels - the feeding and the permeate channels. Different shapes of the channels cross-section were taken into account. Consideration was given to co-current and counter-current flows, for feeding and permeate streams, respectively, flowing together with the inert gas receiving permeate. In the proposed mathematical model it was considered that pressure of gas changes along the length of flow channels was the result of both - the drop of pressure connected with flow resistance, and energy transfer by molecules of gas flowing in a given channel to molecules which penetrate this channel from the adjacent channel. The literature on membrane technology takes into account only the drop of pressure connected with flow resistance. Consideration given to energy transfer by molecules of gas flowing in a given channel to molecules which penetrate this channel from the adjacent channel constitute the essential novelty in the current study. The paper also presents results of calculations obtained by means of a computer program which used equations of the derived model. Physicochemical data concerning separation of the CO2/CH4 mixture with He as the sweep gas and data concerning properties of the membrane made of PDMS were assumed for calculations.

  20. Control of flow separation on a contour bump by jets in a Mach 1.9 free-stream: An experimental study

    Science.gov (United States)

    Lo, Kin Hing; Zare-Behtash, Hossein; Kontis, Konstantinos

    2016-09-01

    Flow separation control over a three-dimensional contour bump using jet in a Mach 1.9 supersonic free-stream has been experimentally investigated using a transonic/supersonic wind tunnel. Jet total pressure in the range of 0-4 bar was blowing at the valley of the contour bump. Schlieren photography, surface oil flow visualisation and particle image velocimetry measurements were employed for flow visualisation and diagnostics. Experimental results show that blowing jet at the valley of the contour bump can hinder the formation and distort the spanwise vortices. The blowing jet can also reduce the extent of flow separation appears downstream of the bump crest. It was observed that this approach of flow control is more effective when high jet total pressure is employed. It is believed that a pressure gradient is generated as a result of the interaction between the flow downstream of the bump crest and the jet induced shock leads to the downwards flow motion around the bump valley.

  1. A study of optimum cowl shapes and flow port locations for minimum drag with effective engine cooling, volume 1

    Science.gov (United States)

    Fox, S. R.; Smetana, F. O.

    1980-01-01

    The contributions to the cruise drag of light aircraft arising from the shape of the engine cowl and the forward fuselage area and also that resulting from the cooling air mass flow through intake and exhaust sites on the nacelle were analyzed. The methods employed for the calculation of the potential flow about an arbitrary three dimensional body are described with modifications to include the effects of boundary layer displacement thickness, a nonuniform onset flow field (such as that due to a rotating propeller), and the presence of air intakes and exhausts. A simple, reliable, largely automated scheme to better define or change the shape of a body is also presented. A technique was developed which can yield physically acceptable skin friction and pressure drag coefficients for isolated light aircraft bodies. For test cases on a blunt nose Cessna 182 fuselage, the technique predicted drag reductions as much as 28.5% by body recontouring and proper placements and sizing of the cooling air intakes and exhausts.

  2. Genetic Algorithm for Solving Location Problem in a Supply Chain Network with Inbound and Outbound Product Flows

    OpenAIRE

    Suprayogi Suprayogi; Senator Nur Bahagia; Yudi Thaddeus

    2016-01-01

    This paper considers a location problem in a supply chain network. The problem addressed in this paper is motivated by an initiative to develop an efficient supply chain network for supporting the agricultural activities. The supply chain network consists of regions, warehouses, distribution centers, plants, and markets. The products include a set of inbound products and a set of outbound products. In this paper, definitions of the inbound and outbound products are seen from the region’s poin...

  3. Studies on Molecular and Ion Transport in Silicalite Membranes and Applications as Ion Separator for Redox Flow Battery

    Science.gov (United States)

    Yang, Ruidong

    Microporous zeolite membranes have been widely studied for molecular separations based on size exclusion or preferential adsorption-diffusion mechanisms. The MFI-type zeolite membranes were also demonstrated for brine water desalination by molecular sieving effect. In this research, the pure silica MFI-type zeolite (i.e. silicalite) membrane has been for the first time demonstrated for selective permeation of hydrated proton (i.e. H3O+) in acidic electrolyte solutions. The silicalite membrane allows for permeation of H 3O+ ions, but is inaccessible to the large hydrated multivalent vanadium ions due to steric effect. The silicalite membrane has been further demonstrated as an effective ion separator in the all-vanadium redox flow battery (RFB).The silicalite is nonionic and its proton conductivity relies on the electric field-driven H3O+ transport through the sub nanometer-sized pores under the RFB operation conditions. The silicalite membrane displayed a significantly reduced self-discharge rate because of its high proton-to-vanadium ion transport selectivity. However, the nonionic nature of the silicalite membrane and very small diffusion channel size render low proton conductivity and is therefore inefficient as ion exchange membranes (IEMs) for practical applications. The proton transport efficiency may be improved by reducing the membrane thickness. However, the zeolite thin films are extremely fragile and must be supported on mechanically strong and rigid porous substrates. In this work, silicalite-Nafion composite membranes were synthesized to achieve a colloidal silicalite skin on the Nafion thin film base. The "colloidal zeolite-ionic polymer" layered composite membrane combines the advantages of high proton-selectivity of the zeolite layer and the mechanical flexibility and low proton transport resistance of the ionic polymer membrane. The composite membrane exhibited higher proton/vanadium ion separation selectivity and lower electrical resistance than

  4. Computed statistics at streamgages, and methods for estimating low-flow frequency statistics and development of regional regression equations for estimating low-flow frequency statistics at ungaged locations in Missouri

    Science.gov (United States)

    Southard, Rodney E.

    2013-01-01

    The weather and precipitation patterns in Missouri vary considerably from year to year. In 2008, the statewide average rainfall was 57.34 inches and in 2012, the statewide average rainfall was 30.64 inches. This variability in precipitation and resulting streamflow in Missouri underlies the necessity for water managers and users to have reliable streamflow statistics and a means to compute select statistics at ungaged locations for a better understanding of water availability. Knowledge of surface-water availability is dependent on the streamflow data that have been collected and analyzed by the U.S. Geological Survey for more than 100 years at approximately 350 streamgages throughout Missouri. The U.S. Geological Survey, in cooperation with the Missouri Department of Natural Resources, computed streamflow statistics at streamgages through the 2010 water year, defined periods of drought and defined methods to estimate streamflow statistics at ungaged locations, and developed regional regression equations to compute selected streamflow statistics at ungaged locations. Streamflow statistics and flow durations were computed for 532 streamgages in Missouri and in neighboring States of Missouri. For streamgages with more than 10 years of record, Kendall’s tau was computed to evaluate for trends in streamflow data. If trends were detected, the variable length method was used to define the period of no trend. Water years were removed from the dataset from the beginning of the record for a streamgage until no trend was detected. Low-flow frequency statistics were then computed for the entire period of record and for the period of no trend if 10 or more years of record were available for each analysis. Three methods are presented for computing selected streamflow statistics at ungaged locations. The first method uses power curve equations developed for 28 selected streams in Missouri and neighboring States that have multiple streamgages on the same streams. Statistical

  5. CENTRIFUGAL SEPARATORS

    Science.gov (United States)

    Skarstrom, C.

    1959-03-10

    A centrifugal separator is described for separating gaseous mixtures where the temperature gradients both longitudinally and radially of the centrifuge may be controlled effectively to produce a maximum separation of the process gases flowing through. Tbe invention provides for the balancing of increases and decreases in temperature in various zones of the centrifuge chamber as the result of compression and expansions respectively, of process gases and may be employed effectively both to neutralize harmful temperature gradients and to utilize beneficial temperaturc gradients within the centrifuge.

  6. Microwave Ablation Using Four-Tine Antenna: Effects of Blood Flow Velocity, Vessel Location, and Total Displacement on Porous Hepatic Cancer Tissue

    Science.gov (United States)

    Chaichanyut, Montree

    2016-01-01

    This research is concerned with microwave ablation analyses using a 2.45 GHz four-tine (4T) antenna for hepatic cancer tissue. In the study, three-dimensional finite-element models were utilized to examine the tissue temperature distributions during and after MW ablation. A preliminary study was first carried out with regard to the specific absorption rates along the 4T antenna insertion depths and the temperature distributions inside the solid and porous liver models with either 3 cm-in-diameter tumor or 5 cm-in-diameter tumor. Based on the preliminary results, the porous models were further examined for the effect of varying blood flow velocities (0–200 cm/s) with a 1 cm-in-diameter blood vessel next to the antenna and also for the effect of vessel-antenna locations (0, 0.8, and 1.3 cm) with a constant blood flow velocity of 16.7 cm/s. All scenarios were simulated under temperature-controlled mode (90°C). The findings revealed that the blood flow velocity and vessel location influence the ablation effectiveness and that increased blood flow inhibits heat transfer to the vessel wall. At the nearest and farthest vessel-antenna locations (0 and 1.3 cm), approximately 90.3% and 99.55% of the cancer cells were eradicated except for the areas adjacent to the vessel. In addition, total tissue thermal displacement is 5.9 mm which is 6.59% of the total length of the overall model. PMID:27642364

  7. Microwave Ablation Using Four-Tine Antenna: Effects of Blood Flow Velocity, Vessel Location, and Total Displacement on Porous Hepatic Cancer Tissue

    Directory of Open Access Journals (Sweden)

    Montree Chaichanyut

    2016-01-01

    Full Text Available This research is concerned with microwave ablation analyses using a 2.45 GHz four-tine (4T antenna for hepatic cancer tissue. In the study, three-dimensional finite-element models were utilized to examine the tissue temperature distributions during and after MW ablation. A preliminary study was first carried out with regard to the specific absorption rates along the 4T antenna insertion depths and the temperature distributions inside the solid and porous liver models with either 3 cm-in-diameter tumor or 5 cm-in-diameter tumor. Based on the preliminary results, the porous models were further examined for the effect of varying blood flow velocities (0–200 cm/s with a 1 cm-in-diameter blood vessel next to the antenna and also for the effect of vessel-antenna locations (0, 0.8, and 1.3 cm with a constant blood flow velocity of 16.7 cm/s. All scenarios were simulated under temperature-controlled mode (90°C. The findings revealed that the blood flow velocity and vessel location influence the ablation effectiveness and that increased blood flow inhibits heat transfer to the vessel wall. At the nearest and farthest vessel-antenna locations (0 and 1.3 cm, approximately 90.3% and 99.55% of the cancer cells were eradicated except for the areas adjacent to the vessel. In addition, total tissue thermal displacement is 5.9 mm which is 6.59% of the total length of the overall model.

  8. Percutaneous coronary intervention using a combination of robotics and telecommunications by an operator in a separate physical location from the patient: an early exploration into the feasibility of telestenting (the REMOTE-PCI study).

    Science.gov (United States)

    Madder, Ryan D; VanOosterhout, Stacie M; Jacoby, Mark E; Collins, J Stewart; Borgman, Andrew S; Mulder, Abbey N; Elmore, Matthew A; Campbell, Jessica L; McNamara, Richard F; Wohns, David H

    2017-01-20

    The present study explores the feasibility of telestenting, wherein a physician operator performs stenting on a patient in a separate physical location using a combination of robotics and telecommunications. Patients undergoing robotic stenting were eligible for inclusion. All manipulations of guidewires, balloons, and stents were performed robotically by a physician operator located in an isolated separate room outside the procedure room housing the patient. Communication between the operating physician and laboratory personnel was via telecommunication devices providing real-time audio and video connectivity. Among 20 patients who consented to participate, technical success, defined as successful advancement and retraction of guidewires, balloons, and stents by the robotic system without conversion to manual operation, was achieved in 19 of 22 lesions (86.4%). Procedural success, defined as robotics will facilitate telestenting over greater geographic distances.

  9. Effects of land use and sample location on nitrate-stream flow hysteresis descriptors during storm events

    Science.gov (United States)

    Feinson, Lawrence S.; Gibs, Jacob; Imbrigiotta, Thomas E.; Garrett, Jessica D.

    2016-01-01

    The U.S. Geological Survey's New Jersey and Iowa Water Science Centers deployed ultraviolet-visible spectrophotometric sensors at water-quality monitoring sites on the Passaic and Pompton Rivers at Two Bridges, New Jersey, on Toms River at Toms River, New Jersey, and on the North Raccoon River near Jefferson, Iowa to continuously measure in-stream nitrate plus nitrite as nitrogen (NO3 + NO2) concentrations in conjunction with continuous stream flow measurements. Statistical analysis of NO3 + NO2 vs. stream discharge during storm events found statistically significant links between land use types and sampling site with the normalized area and rotational direction of NO3 + NO2-stream discharge (N-Q) hysteresis patterns. Statistically significant relations were also found between the normalized area of a hysteresis pattern and several flow parameters as well as the normalized area adjusted for rotational direction and minimum NO3 + NO2 concentrations. The mean normalized hysteresis area for forested land use was smaller than that of urban and agricultural land uses. The hysteresis rotational direction of the agricultural land use was opposite of that of the urban and undeveloped land uses. An r2 of 0.81 for the relation between the minimum normalized NO3 + NO2 concentration during a storm vs. the normalized NO3 + NO2 concentration at peak flow suggested that dilution was the dominant process controlling NO3 + NO2 concentrations over the course of most storm events.

  10. Observations of Correlated Behavior of Two Light Torsion Balances and a Paraconical Pendulum in Separate Locations during the Solar Eclipse of January 26th, 2009

    Directory of Open Access Journals (Sweden)

    A. F. Pugach

    2012-01-01

    Full Text Available On January 26th, 2009, simultaneous observations of the reactions of two very light torsion balances (Kiev, Ukraine and a paraconical pendulum (Suceava, Romania, 440 km away were performed during a solar eclipse that was not visible at those locations but only in the Indian Ocean. Significant correlation between the behavior of the torsion balances and the pendulum was observed. The conclusion is that a solar eclipse influences the reactions of torsion balances and pendulums, even in areas of the Earth where it is not optically visible.

  11. The association between body-built and injury occurrence in pre-professional ballet dancers – Separated analysis for the injured body-locations

    Directory of Open Access Journals (Sweden)

    Petra Zaletel

    2017-02-01

    Full Text Available Objectives: This study has aimed at identifying prevalence of injury-occurrence in 24 pre-professional-ballet-dancers (females, 16–18 years of age, and identifying the associations between the body-built and prevalence of injuries. Material and Methods: The sample of variables included: body mass, body height, and 3 somatotype characteristics (mesomorph, ectomorph and endomorph and data on injuries over the preceding year. Results: Dancers were mostly ectomorphic-mesomorph (endomorphy: 2.6±0.54, mesomorphy: 3.99±0.77, ectomorphy: 3.23±0.54. The most commonly injured locations were the foot (17% of all injuries and ankle (17%. Majority of the injuries occurred while practising but 37% of hip-injuries occurred while performing. Ankle-injuries resulted in longest absence from ballet. Endomorphy was related to ankle-injury (odds ratio (OR = 1.9, 95% confidence interval (CI: 1.4–2.3, ectomorphy to foot injury (OR = 1.7, 95% CI: 1.1–2.9, and body-mass to injury to the toes (OR = 1.7, 95% CI: 1.4–3.1. Conclusions: The results of this study allow for recognizing those dancers who are particularly vulnerable to injuries of certain body location. A more profound analysis of the possible mechanisms that lead to hip-injury during performance is needed. Int J Occup Med Environ Health 2017;30(1:151–159

  12. Visualization of boundary layer separation and passive flow control on airfoils and bodies in wind-tunnel and in-flight experiments

    Science.gov (United States)

    Popelka, Lukas; Kuklova, Jana; Simurda, David; Souckova, Natalie; Matejka, Milan; Uruba, Vaclav

    2012-04-01

    Infrared camera, Particle Image Velocimetry, smoke-wire, tuft filaments and oil-flow visualization techniques were used for wind-tunnel and in-flight investigation of boundary layer separation, both stall and separation bubbles, related to the low-Reynolds numbers transition mechanism. Airfoils of Wortmann FX66 series and FX66 series wing-fuselage interaction, as well as modern airfoils and their wing-fuselage geometry were subject to study. The presence of previously identified structures in the CFD modelling, such as horse-shoe vortices, was confirmed in the flow. Wind-tunnels and in-flight measurements on sailplanes were carried out and effect of passive flow control devices - vortex generators - was surveyed; namely counter-rotating vortex generators and Zig-zag type turbulators were applied. Separation suppression and consequent drag coefficient reduction of test aircrafts was reached. PIV investigation was further extended by Time-Resolved techniques. An important study on structure of the turbulent flow in the lower atmosphere, creating an environment of the soaring flight, was presented.

  13. Visualization of boundary layer separation and passive flow control on airfoils and bodies in wind-tunnel and in-flight experiments

    Directory of Open Access Journals (Sweden)

    Matejka Milan

    2012-04-01

    Full Text Available Infrared camera, Particle Image Velocimetry, smoke-wire, tuft filaments and oil-flow visualization techniques were used for wind-tunnel and in-flight investigation of boundary layer separation, both stall and separation bubbles, related to the low-Reynolds numbers transition mechanism. Airfoils of Wortmann FX66 series and FX66 series wing-fuselage interaction, as well as modern airfoils and their wing-fuselage geometry were subject to study. The presence of previously identified structures in the CFD modelling, such as horse-shoe vortices, was confirmed in the flow. Wind-tunnels and in-flight measurements on sailplanes were carried out and effect of passive flow control devices - vortex generators - was surveyed; namely counter-rotating vortex generators and Zig-zag type turbulators were applied. Separation suppression and consequent drag coefficient reduction of test aircrafts was reached. PIV investigation was further extended by Time-Resolved techniques. An important study on structure of the turbulent flow in the lower atmosphere, creating an environment of the soaring flight, was presented.

  14. Increment of the operational stability of the NGL I plant located in the Tablazo Petrochemical Complex, working with a low feed gas flow rate

    Energy Technology Data Exchange (ETDEWEB)

    Mendez, Alejandro

    2004-07-01

    The function of the plant NGL I is to extract the ethane and the liquids of the current of natural gas coming from Maracaibo Lake, emphatically in the ethane and propane for being a raw material for the olefins synthesis. The needed temperature for the condensation takes place cooling the dehydrated feed gas, by means of the heat exchange with two currents, cooling propane and the other cold methane gas. In 1998 some improvements to the plant were made, which are known as (MERE) (Ethane Recovery Improvements Project), these modifications were made in order to enhance the ethane recovery during the process, however, when it started to work under this modifications high instability in the performance of the compressor located in the refrigeration system was observed, which produced the shutdown of the ethane recovery process when working with feed gas stream flow rates (under 130 MMSCFD). Moreover, during the last years a decrease in the feed gas flow has been observed, in a mean of 120 MMSCFD, which has had a negative effect on the plant, because it has worked in a non-continuous state. Thanks to several improvements implemented in the actuality, we have achieved to diminish the minimal flow of operation of the plant to 95 MMPCSD, allowing the ethane production at this flow of feeding and increased the operational stability of the plant of LNG I. (author)

  15. Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing.

    Science.gov (United States)

    Jezierski, Stefan; Klein, Anke S; Benz, Christian; Schaefer, Michael; Nagl, Stefan; Belder, Detlev

    2013-06-01

    We immobilized adherent human embryonic kidney (HEK) cells--which are able to trace adenosine triphosphate (ATP)--inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.

  16. An auroral westward flow channel (AWFC and its relationship to field-aligned current, ring current, and plasmapause location determined using multiple spacecraft observations

    Directory of Open Access Journals (Sweden)

    M. L. Parkinson

    2007-02-01

    Full Text Available An auroral westward flow channel (AWFC is a latitudinally narrow channel of unstable F-region plasma with intense westward drift in the dusk-to-midnight sector ionosphere. AWFCs tend to overlap the equatorward edge of the auroral oval, and their life cycle is often synchronised to that of substorms: they commence close to substorm expansion phase onset, intensify during the expansion phase, and then decay during the recovery phase. Here we define for the first time the relationship between an AWFC, large-scale field-aligned current (FAC, the ring current, and plasmapause location. The Tasman International Geospace Environment Radar (TIGER, a Southern Hemisphere HF SuperDARN radar, observed a jet-like AWFC during ~08:35 to 13:28 UT on 7 April 2001. The initiation of the AWFC was preceded by a band of equatorward expanding ionospheric scatter (BEES which conveyed an intense poleward electric field through the inner plasma sheet. Unlike previous AWFCs, this event was not associated with a distinct substorm surge; rather it occurred during an interval of persistent, moderate magnetic activity characterised by AL~−200 nT. The four Cluster spacecraft had perigees within the dusk sector plasmasphere, and their trajectories were magnetically conjugate to the radar observations. The Waves of High frequency and Sounder for Probing Electron density by Relaxation (WHISPER instruments on board Cluster were used to identify the plasmapause location. The Imager for Magnetopause-to-Aurora Global Exploration (IMAGE EUV experiment also provided global-scale observations of the plasmapause. The Cluster fluxgate magnetometers (FGM provided successive measurements specifying the relative location of the ring current and filamentary plasma sheet current. An analysis of Iridium spacecraft magnetometer measurements provided estimates of large-scale ionospheric FAC in relation to the AWFC evolution. Peak flows in the AWFC were located close to the peak of a Region 2

  17. Separation of basic proteins from Leishmania using a combination of Free flow electrophoresis (FFE) and 2D electrophoresis (2-DE) under basic conditions.

    Science.gov (United States)

    Brotherton, Marie-Christine; Racine, Gina; Ouellette, Marc

    2015-01-01

    Basic proteins, an important class of proteins in intracellular organisms such as Leishmania, are usually underrepresented on 2D gels. This chapter describes a method combining basic proteins fractionation using Free flow electrophoresis in isoelectric focusing mode (IEF-FFE) followed by protein separation using two-dimensional gel electrophoresis (2-DE) in basic conditions. The combination of these two techniques represents a great improvement for the visualization of Leishmania proteins with basic pI using 2D gels.

  18. Role of hydrodynamic factors in controlling the formation and location of unconformity-related uranium deposits: insights from reactive-flow modeling

    Science.gov (United States)

    Aghbelagh, Yousef Beiraghdar; Yang, Jianwen

    2017-03-01

    The role of hydrodynamic factors in controlling the formation and location of unconformity-related uranium (URU) deposits in sedimentary basins during tectonically quiet periods is investigated. A number of reactive-flow modeling experiments at the deposit scale were carried out by assigning different dip angles and directions to a fault and various permeabilities to hydrostratigraphic units). The results show that the fault dip angle and direction, and permeability of the hydrostratigraphic units govern the convection pattern, temperature distribution, and uranium mineralization. A vertical fault results in uranium mineralization at the bottom of the fault within the basement, while a dipping fault leads to precipitation of uraninite below the unconformity either away from or along the plane of the fault, depending on the fault permeability. A more permeable fault causes uraninite precipitates along the fault plane, whereas a less permeable one gives rise to the precipitation of uraninite away from it. No economic ore mineralization can form when either very low or very high permeabilities are assigned to the sandstone or basement suggesting that these units seem to have an optimal window of permeability for the formation of uranium deposits. Physicochemical parameters also exert an additional control in both the location and grade of URU deposits. These results indicate that the difference in size and grade of different URU deposits may result from variation in fluid flow pattern and physicochemical conditions, caused by the change in structural features and hydraulic properties of the stratigraphic units involved.

  19. Microgravity Passive Phase Separator

    Science.gov (United States)

    Paragano, Matthew; Indoe, William; Darmetko, Jeffrey

    2012-01-01

    A new invention disclosure discusses a structure and process for separating gas from liquids in microgravity. The Microgravity Passive Phase Separator consists of two concentric, pleated, woven stainless- steel screens (25-micrometer nominal pore) with an axial inlet, and an annular outlet between both screens (see figure). Water enters at one end of the center screen at high velocity, eventually passing through the inner screen and out through the annular exit. As gas is introduced into the flow stream, the drag force exerted on the bubble pushes it downstream until flow stagnation or until it reaches an equilibrium point between the surface tension holding bubble to the screen and the drag force. Gas bubbles of a given size will form a front that is moved further down the length of the inner screen with increasing velocity. As more bubbles are added, the front location will remain fixed, but additional bubbles will move to the end of the unit, eventually coming to rest in the large cavity between the unit housing and the outer screen (storage area). Owing to the small size of the pores and the hydrophilic nature of the screen material, gas does not pass through the screen and is retained within the unit for emptying during ground processing. If debris is picked up on the screen, the area closest to the inlet will become clogged, so high-velocity flow will persist farther down the length of the center screen, pushing the bubble front further from the inlet of the inner screen. It is desired to keep the velocity high enough so that, for any bubble size, an area of clean screen exists between the bubbles and the debris. The primary benefits of this innovation are the lack of any need for additional power, strip gas, or location for venting the separated gas. As the unit contains no membrane, the transport fluid will not be lost due to evaporation in the process of gas separation. Separation is performed with relatively low pressure drop based on the large surface

  20. Test and Analysis on Flow Field in A Two-Phase Flow Water-Sediment Separation Device by PIV Techniques%两相流水沙分离鳃流场的PIV测试及分析

    Institute of Scientific and Technical Information of China (English)

    赵丽娜; 邱秀云; 陈铂; 邹强

    2012-01-01

    利用粒子图像测速技术,测试了两种工况下垂向异重流式水沙分离鳃中水沙两相流的速度场,给出了分离鳃内泥沙运动的流速矢量分布图、流速云图及三维流场分布图.对测试结果的分析表明,分离鳃内部形成了泥沙沿鳃片上表面向下运动,清水沿鳃片下表面向上运动的垂向异重流现象,加速了泥沙的下沉.此分析结果与实际观察现象及理论分析一致,进一步探明了分离鳃的水沙分离机理.%A Particle Image Velocimetry (short for PIV) technology was used to test the velocity field of water-sediment two-phase flow in a vertical-component density flow water-sediment separation device under two working conditions, and the distribution map of velocity vector of sediment movement in separation device,cloud atlas of velocity and distribution map of three-dimentional flow field were presented. Analysis of the test results indicated that a vertical-component density flow was formed in the separation device,and its phenomena was follows as-the sediment moved down along the upper surface of the gill slice and the water moved up along the undersurface oppositely. This analysis result was in accordance with the real observed phenomena and the theoretical analysis result, which further proved the separation mechanism of water and sediment in the separation device.

  1. Stochastic estimation of a two/three-dimensional separating/reattaching boundary layer flow field downstream of an axisymmetric backward-facing step

    Science.gov (United States)

    Hudy, Laura; Naguib, Ahmed; Humphreys, William; Bartram, Scott

    2004-11-01

    The spatio-temporal characteristics of a separating/reattaching flow region were investigated. The experimental set-up consisted of an axisymmetric backward-facing step with a section upstream of the step that could rotate to create a 3D boundary layer at separation. Beneath the flow region, 32 flush-mounted microphones detected the pressure signature that was acquired simultaneously with PIV measurements, which captured the velocity field above the surface. Data were obtained for different Reynolds numbers (Re), based on step height: five Re for the 2D flow case, ranging from 6100 to 33200, and two Re, 6100 and 8300, for the 3D case. Multi-point stochastic estimation was used to estimate the dominant flow structures above the wall from the wall-pressure signature and the results were compared with the instantaneous structures obtained from the PIV measurements. In addition, Re effects were explored in the 2D case. Supported by NSF grant CTS0116907 and partial support from ONR

  2. Importance of flow stratification and bubble aggregation in the separation zone of a dissolved air flotation tank.

    Science.gov (United States)

    Lakghomi, B; Lawryshyn, Y; Hofmann, R

    2012-09-15

    The importance of horizontal flow patterns and bubble aggregation on the ability of dissolved air flotation (DAF) systems to improve bubble removal during drinking water treatment were explored using computational fluid dynamics (CFD) modeling. Both analytical and CFD analyses demonstrated benefits to horizontal flow. Two dimensional CFD modeling of a DAF system showed that increasing the amount of air in the system improved the bubble removal and generated a beneficial stratified horizontal flow pattern. Loading rates beyond a critical level disrupted the horizontal flow pattern, leading to significantly lower bubble removal. The results also demonstrated that including the effects of bubble aggregation in CFD modeling of DAF systems is an essential component toward achieving realistic modeling results.

  3. A direct numerical simulation investigation of the synthetic jet frequency effects on separation control of low-Re flow past an airfoil

    KAUST Repository

    Zhang, Wei

    2015-05-05

    We present results of direct numerical simulations of a synthetic jet (SJ) based separation control of flow past a NACA-0018 (National Advisory Committee for Aeronautics) airfoil, at 10° angle of attack and Reynolds number 104 based on the airfoil chord length C and uniform inflow velocity U 0. The actuator of the SJ is modeled as a spanwise slot on the airfoil leeward surface and is placed just upstream of the leading edge separation position of the uncontrolled flow. The momentum coefficient of the SJ is chosen at a small value 2.13 × 10−4 normalized by that of the inflow. Three forcing frequencies are chosen for the present investigation: the low frequency (LF) F + = feC/U 0 = 0.5, the medium frequency (MF) F + = 1.0, and the high frequency (HF) F + = 4.0. We quantify the effects of forcing frequency for each case on the separation control and related vortex dynamics patterns. The simulations are performed using an energy conservative fourth-order parallel code. Numerical results reveal that the geometric variation introduced by the actuator has negligible effects on the mean flow field and the leading edge separation pattern; thus, the separation control effects are attributed to the SJ. The aerodynamic performances of the airfoil, characterized by lift and lift-to-drag ratio, are improved for all controlled cases, with the F + = 1.0 case being the optimal one. The flow in the shear layer close to the actuator is locked to the jet, while in the wake this lock-in is maintained for the MF case but suppressed by the increasing turbulent fluctuations in the LF and HF cases. The vortex evolution downstream of the actuator presents two modes depending on the frequency: the vortex fragmentation and merging mode in the LF case where the vortex formed due to the SJ breaks up into several vortices and the latter merge as convecting downstream; the discrete vortices mode in the HF case where discrete vortices form and convect downstream without any fragmentation and

  4. 旋流器内气相时均流场的试验研究%Experimental Study on the Time-mean Flow Field in Cyclone Separator

    Institute of Scientific and Technical Information of China (English)

    李虹; 金向红

    2011-01-01

    Time-averaged velocities of three-dimensional turbulence flow field in a cyclone separator were measured by using Laser Doppler Velocimeter.The effect of different guide vane angle and flow rate on the time-mean flow field has been studied.In the separation space, the results show that the tangential velocity profiles have a typical Rankine vortex structure, and the tangential velocity decay slightly with axial distance.The axial velocity profiles is constructed by outer downward flow and inner upward flow, and the axial velocity decay with axial distance.The profiles of the maximal tangential velocity and axial LZVV exhibit likeness to the cylinder-on-cone structure of the vessel.The features of the time-mean flow field in the annual space and liquid chamber were discussed and analysed.%采用多普勒激光测速仪对旋流分离器内三维湍流的时均流场进行了测量,考察了导叶导角和流量变化对时均流场的影响.测量结果表明:在分离空间,切向速度分布呈现典型的Rankin涡结构,切向速度沿轴向衰减不明显,轴向速度是由外围的下行流与内部的上行流结构,轴向速度沿轴向衰减.最大切向速度面与轴向LZVV面呈现与筒体相似的管锥形.对环形空间和集液槽内时均流场的结构也进行了分析.

  5. Investigation, comparison and design of chambers used in centrifugal partition chromatography on the basis of flow pattern and separation experiments.

    Science.gov (United States)

    Schwienheer, C; Merz, J; Schembecker, G

    2015-04-17

    In centrifugal partition chromatography (CPC) the separation efficiency is mainly influenced by the hydrodynamic of mobile and stationary phase in the chambers. Thus, the hydrodynamic has to be investigated and understood in order to enhance a CPC separation run. Different chamber geometries have been developed in the past and the influence of several phase systems and CPC operating conditions were investigated for these chambers. However, a direct comparison between the different chamber types has not been performed yet. In order to investigate the direct influence of the chamber design on the hydrodynamic, several chamber designs - partially similar in geometry to commercial available designs - are investigated under standardized conditions in the present study. The results show the influence of geometrical aspects of the chamber design on the hydrodynamic and therewith, on the separation efficiency. As a conclusion of the present study, some ideas for an optimal chamber design for laboratory and industrial purpose are proposed.

  6. Controlling Separation in Turbomachines

    Science.gov (United States)

    Evans, Simon; Himmel, Christoph; Power, Bronwyn; Wakelam, Christian; Xu, Liping; Hynes, Tom; Hodson, Howard

    2010-01-01

    Four examples of flow control: 1) Passive control of LP turbine blades (Laminar separation control). 2) Aspiration of a conventional axial compressor blade (Turbulent separation control). 3) Compressor blade designed for aspiration (Turbulent separation control). 4.Control of intakes in crosswinds (Turbulent separation control).

  7. Continuous-flow separation and pre-concentration coupled on-line to solid-surface fluorescence spectroscopy for the simultaneous determination of o-phenylphenol and thiabendazole.

    Science.gov (United States)

    García Reyes, J F; Llorent Martínez, E J; Ortega Barrales, P; Molina Díaz, A

    2004-01-01

    A novel and single flow-injection system combined with solid-surface fluorescence detection is proposed in this work for the resolution of a mixture of two widely used pesticides (o-phenylphenol and thiabendazole). The continuous-flow methodology is based on the implementation of on-line pre-concentration and separation of both analytes on the surface of C18 silica gel beads placed just inside the flow cell, implemented with gel-phase fluorimetric multi-wavelength detection (using 305/358 and 250/345 nm as excitation/emission wavelengths for thiabendazole and o-phenylphenol, respectively). The separation of the pesticides was possible owing to the different retention/desorption kinetics of their interactions with the solid support in the zone where the stream impinges on the solid material. No previous separation of the analytes before they reach the flow cell is needed thereby simplifying substantially both the procedure and the manifold. By using a sample volume of 2,600 microL, the system was calibrated in the range 0.5-16 and 5-120 ng mL(-1) with detection limits of 0.09 and 0.60 ng mL(-1) for thiabendazole and o-phenylphenol, respectively. The RSD values (n=10) were about 1% for both analytes. The proposed methodology was applied to environmental water samples and also to various commercial pesticide formulations containing both analytes. Recovery percentages were 97-103% and 98-102% for thiabendazole and o-phenylphenol, respectively.

  8. A method for the separation of total discharge into base flow, overland flow and channel precipitation for water quality modelling of a small watershed in the Netherlands

    NARCIS (Netherlands)

    Bleuten, W.

    1988-01-01

    For surface water quality modelling all contributing discharges, each with different loads of dissolved matter have to be considered separately. Apart from physical and (bio)chemical interactions, water quality is the result of all inputs, both in volume and mass. For this reason dynamic modelling o

  9. A 2D Simulation of the Flow Separation Control over a NACA0015 Airfoil Using a Synthetic Jet Actuator

    Science.gov (United States)

    Boukenkoul, M. A.; Li, F. C.; Aounallah, M.

    2017-03-01

    The present study aims to investigate numerically the flow control possibility using a synthetic jet actuation over a bi-dimensional NACA0015 airfoil manoeuvring at a highly turbulent flow (8.9e105 Reynolds to chord number). The 2-D flow behaviour was computed using the ANSYS Fluent commercial code. The so-called Reynolds Averaged Navier-Stocks (RANS) approach has been tested for one (Spalat-Allmaras S-A) and two (K-ε) transport equations for the turbulence modelling. Both present a weakness to predict the stall angle effectively. The S-A lift coefficient slope seems to be the closest to the experimental data. The synthetic jet control exhibits an extraordinary lift coefficient enhancement at high Angles Of Attack (AOA) but seems to be less obvious at low AOA, where the flow is still attached. A synthetic jet of a Strouhal (St = 2) and momentum (Cμ of 0.56%), delays the stall onset from 15 to 19deg with enhancing the lift coefficient by 40%. The actual work has been enriched by studying the effect of the jet’s frequency and momentum on the lift temporal signal. Also, the interaction between the mean flow and the synthetic jet structures topology was undertaken.

  10. A three-dimensional boundary-layer method for flow over delta wings with leading-edge separation

    Science.gov (United States)

    Woodson, S. H.; Dejarnette, F. R.

    1985-01-01

    A three-dimensional, laminar boundary-layer method is applied to the incompressible flow over a slender delta wing at incidence. The predictor-corrector finite-difference scheme of Matsuno is used to difference the governing equations. The method has the advantages that no iterations are required to advance the solution and the cross-flow derivatives are formed independent of the cross-field direction. The difference scheme is demonstrated to yield accurate numerical results when compared to the exact solution of the three-dimensional boundary-layer equations for parabolic flow over a moving flat plate. The method is applied to delta wings of various sweep angles at angles of attack up to 20 deg., with the inviscid solution determined using a higher-order, three-dimensional panel method.

  11. Development of an Immunomagnetic Separation Method for Viable Salmonella Typhimurium Detected by Flow Cytometry

    DEFF Research Database (Denmark)

    Ahmed, Shakil; Rubahn, Horst-Günter; Erdmann, Helmut

    2016-01-01

    and bacteria, immunocapture time, staining and buffering conditions for the viability assays were optimized. The capture efficiency of IMS was>98% for a range of Salmonella Typhimurium cell concentrations from 103 to 105/mL using 108/mL bead concentration. The method proved to have high (98%) specificity...... paramagnetic particles for the preparation of immunomagnetic beads (IMBs). The most suitable antibody was chosen by applying an enzyme linked immunosorbent assay (ELISA), whereas living bacteria were detected by flow cytometry. The parameters for both IMS and flow cytometry e.g., concentration of bead...

  12. 两级旋风分离器内气固两相流动及分离特性%Double-phase flow and separation features in a two-phase cyclone separator

    Institute of Scientific and Technical Information of China (English)

    闫云飞; 张力; 刘科

    2011-01-01

    This paper is engaged in a study of the double-phase flow and separator features in a double-phase cyclone separator in hope to increase the separation efficiency of small density particles below 30 μm. For this purpose, we have done numerical analyses of the threedimensional flow and separation activities of the dust and air in the modified two-phase cyclone by using the κ- ε/RNG and DPM medel.Due to the difficult separation of minute particles, the numerical simulation method can be used to simulate the particle motion in a cyclone separator. The particle phase can be actually taken as a discrete phase. However, since the gas phase is seen as a continuous one, the κ- ε turbulence model can be used for the intended flow simulation so as to gain the velocity distribution, pressure distribution and the separation efficiency. The result of simulation indicate that it appears upper and lower diversion into the inner cylinder of twophased separator, which is different from the conventional one. In the process we found that the swirling turbulence emerged near the wall of the outer cylinder and conic zone, though there were no stagnation and refiow detected on the central axis. Whereas the axial velocity distribution is non-axial symmetric, the axial symmetry was found to the tangential velocity distribution at the same height. In addition,the axial velocity distribution tended to be different with more consistent tangential velocity distribution at the different heights. We have also discovered that with the increase of particle size, the escaping efficiency tends to decrease. Moreover, with the separation efficiency rising, the catch efficiency first increased but then decreased. The separation efficiency of 5 μm particles can be made to reach over 60%, which is indeed a great improvement in comparison with the conventional separator. Thus, it can be concluded that the key factors leading to the improvement of separation efficiency are the increase of movement

  13. On-line extractive separation in flow injection analysis based on polymer inclusion membranes: a study on membrane stability and approaches for improving membrane permeability.

    Science.gov (United States)

    Zhang, Lujia L; Cattrall, Robert W; Ashokkumar, Muthupandian; Kolev, Spas D

    2012-08-15

    The effect of temperature on the sensitivity and sampling rate is studied for a flow injection analysis (FIA) system that uses a membrane separation cell fitted with a polymer inclusion membrane (PIM) for the determination of Zn(II). A temperature of 50 °C for the flowing donor and acceptor solutions and the membrane separation cell improves the sensitivity and the sampling rate relative to 20 °C up to 10-fold and 2-fold, respectively. Studies on the stability of the PIM are reported that show a limited loss of the membrane liquid phase into the aqueous phases used in the FIA system but this has exhibited a negligible effect on the amount of Zn(II) transported across the membrane. Most importantly, the extent of leaching of the PIM components is shown to depend on the nature of the aqueous phase with the membrane eventually reaching a stable composition. It is also shown that the application of ultrasound to the membrane separation cell leads to a slight increase in sensitivity without affecting the long term membrane stability.

  14. Focused-microwave-assisted reaction in flow injection spectrophotometry: a new liquid-vapor separation chamber for determination of reducing sugars in wine.

    Science.gov (United States)

    Oliveira, A F; Fatibello-Filho, O; Nóbrega, J A

    2001-10-31

    The usefulness of an unsegmented liquid/vapor phases flow injection system for determining reducing sugars in wines using a focalized poly(tetrafluoroethylene) (PTFE) coiled reactor positioned at the output antenna of a domestic microwave oven at 700 W is demonstrated. In this system, sample or reference solution is mixed to 2.5 mol l(-1) sodium hydroxide and 6.8 mmol l(-1) potassium hexacyanoferrate(III) solutions previously merged in a confluence point and the reducing sugars (glucose plus fructose) were oxidized by potassium hexacyanoferrate(III) solution into the PTFE coiled reactor positioned in the microwave oven cavity. After phases separation at a proposed separation chamber (SC), the decrease of K(3)Fe(CN)(6) concentration in the liquid phase was measured at 420 nm. Using this SC, a favorable dispersion profile and a decrease of hydrodynamic pressure were observed. The flow procedure presented similar sensitivities to glucose and fructose in the experimental conditions adopted, allowing the determination of the reducing sugars content (fructose plus glucose concentrations) in the concentration range from 40 to 400 mumol l(-1) with a detection limit of 9 mumol l(-1). The RSD was 1.9% for a 120 mumol l(-1) fructose solution (n=12) and the analytical frequency was 54 h(-1). The results obtained for reducing sugars in several wines using the proposed flow system and those obtained using the Somogyi-Nelson batch procedure were in agreement at the 95% confidence level.

  15. Rupture mechanism and interface separation in foam rubber models of earthquakes: a possible solution to the heat flow paradox and the paradox of large overthrusts

    Science.gov (United States)

    Brune, James N.; Brown, Stephen; Johnson, Peggy A.

    1993-02-01

    Spontaneous stick-slip along the interface between stressed foam rubber blocks is a simple analog of earthquake rupture and stick-slip. Results from this model are used to elucidate the role of normal stress variations along the interface in the stick-slip process. Observations indicate significant normal interface vibrations and separation during slip, suggesting that dynamic changes in normal stress (rather than a drop in the coefficient of friction) may control stick-slip, as suggested, for example, by Tolstoi, Oden and Martins and Brune and co-workers. Observations of particle trajectories indicate that stick-slip shear motion is associated with various degrees of fault separation. For an asymmetric model, the motion is consistent with slipping motion of the type suggested by Schallamach and Price. For a symmetric model, the motion is similar to that suggested by Comninou and Dundurs. If interface waves of this type, involving separation during slip, occur in earthquakes, they may be a solution to the heat flow paradox, since a major part of the slip occurs during separation and during low normal stress. Thus frictional heat generation is reduced. Normal interface vibrations during stick-slip may explain the high corner frequency of P wave spectra and the generally high levels of P wave spectra beyond the corner frequency. Schallamach-Comninou type waves are consistent with the partial stress drop-abrupt locking-self healing models of Brune and Heaton.

  16. Scale separation for multi-scale modeling of free-surface and two-phase flows with the conservative sharp interface method

    Energy Technology Data Exchange (ETDEWEB)

    Han, L.H., E-mail: Luhui.Han@tum.de; Hu, X.Y., E-mail: Xiangyu.Hu@tum.de; Adams, N.A., E-mail: Nikolaus.Adams@tum.de

    2015-01-01

    In this paper we present a scale separation approach for multi-scale modeling of free-surface and two-phase flows with complex interface evolution. By performing a stimulus-response operation on the level-set function representing the interface, separation of resolvable and non-resolvable interface scales is achieved efficiently. Uniform positive and negative shifts of the level-set function are used to determine non-resolvable interface structures. Non-resolved interface structures are separated from the resolved ones and can be treated by a mixing model or a Lagrangian-particle model in order to preserve mass. Resolved interface structures are treated by the conservative sharp-interface model. Since the proposed scale separation approach does not rely on topological information, unlike in previous work, it can be implemented in a straightforward fashion into a given level set based interface model. A number of two- and three-dimensional numerical tests demonstrate that the proposed method is able to cope with complex interface variations accurately and significantly increases robustness against underresolved interface structures.

  17. An improved cell separation technique for marine subsurface sediments: applications for high-throughput analysis using flow cytometry and cell sorting.

    Science.gov (United States)

    Morono, Yuki; Terada, Takeshi; Kallmeyer, Jens; Inagaki, Fumio

    2013-10-01

    Development of an improved technique for separating microbial cells from marine sediments and standardization of a high-throughput and discriminative cell enumeration method were conducted. We separated microbial cells from various types of marine sediment and then recovered the cells using multilayer density gradients of sodium polytungstate and/or Nycodenz, resulting in a notably higher percent recovery of cells than previous methods. The efficiency of cell extraction generally depends on the sediment depth; using the new technique we developed, more than 80% of the total cells were recovered from shallow sediment samples (down to 100 meters in depth), whereas ~50% of cells were recovered from deep samples (100-365 m in depth). The separated cells could be rapidly enumerated using flow cytometry (FCM). The data were in good agreement with those obtained from manual microscopic direct counts over the range 10(4)-10(8) cells cm(-3). We also demonstrated that sedimentary microbial cells can be efficiently collected using a cell sorter. The combined use of our new cell separation and FCM/cell sorting techniques facilitates high-throughput and precise enumeration of microbial cells in sediments and is amenable to various types of single-cell analyses, thereby enhancing our understanding of microbial life in the largely uncharacterized deep subseafloor biosphere.

  18. Separation and quantification of {sup 238}U, {sup 232}Th and rare earths in monazite samples by ion chromatography coupled with on-line flow scintillation detector

    Energy Technology Data Exchange (ETDEWEB)

    Borai, E.H.; Mady, A.S

    2002-10-01

    An alternative procedure has been described for efficient separation and quantitative determination of uranium (U), thorium (Th) and rare earth elements (REEs) in monazite mineral by high performance ion chromatographic system (IC). Different variables affecting the distribution coefficient, the retention and hence separation efficiency such as eluent flow rate and concentrations of HCl and ammonium sulfate (NH{sub 4}){sub 2}SO{sub 4} containing the mobile phase were studied. The developed separation protocol of U, Th and REEs was optimized using an Ion Pac CS5 separation column followed by post-column derivatization reaction with Arsenaso III and UV-VIS spectrophotometeric detection. Comparative evaluation of U, Th and REEs concentration in monazite mineral was demonstrated using two different on-line detection modes including flow through cell scintillation detector (FSD) and UV-VIS spectrophotometer. Response of the FSD was found to be linear over the full range of activities investigated (4-100 Bq). The lower limits of detection (LLD) for the investigated radionuclides were 3.0{+-}0.1 Bq for {sup 238}U and 6.0{+-}0.1 Bq for {sup 232}Th. The detection of {sup 238}U and {sup 232}Th by FSD shows good agreement with the corresponding determination by UV-VIS spectrophotometer. The content of ThO{sub 2} and U{sub 3}O{sub 8} in monazite sample were found to be 4.7{+-}0.1% and 0.42{+-}0.04% using UV-VIS detector and 4.3{+-}0.1% and 0.45{+-}0.1% using FSD, respectively.

  19. Principles of bone marrow processing and progenitor cell/mononuclear cell concentrate collection in a continuous flow blood cell separation system.

    Science.gov (United States)

    Hester, J P; Rondón, G; Huh, Y O; Lauppe, M J; Champlin, R E; Deisseroth, A B

    1995-08-01

    The application of continuous flow apheresis technology to processing bone marrow for collection of the mononuclear progenitor cell population appears to follow the same principles as collection of mononuclear cells from peripheral blood. Unlike peripheral blood, however, where mobilization of cells from extravascular sites during the procedures contributes significantly to the final cell yield, the entire quantity of progenitor cells available for recovery from marrow is present in the original marrow when it is pooled. The process then becomes one of attempting optimal recovery of the cells of interest while excluding contaminating erythrocytes and cells of the myeloid series. This study reports the development of a protocol for recovery of MNC, CD33+, CD34+, and CD34+/DR- cells from harvested marrow for autologous and allogeneic transplants using a continuous flow blood cell separator, the variables influencing the recovery of the cells of interest and the clinical response to infusion of the processed cells.

  20. SIMULTANEOUS PIV/PTV MEASUREMENTS OF BUBBLE AND PARTICLE PHASES IN GAS-LIQUID TWO-PHASE FLOW BASED ON IMAGE SEPARATION AND RECONSTRUCTION

    Institute of Scientific and Technical Information of China (English)

    QU Jian-wu; MURAI Yuichi; YAMAMOTO Fujio

    2004-01-01

    A Particle Image Velocimetry (PIV) method based on the image separation and reconstruction with the median filter and triangular Bézier patch was proposed to measure multiple velocity fields from single-camera images in the present study. The method was examined on synthetic PIV images with the Green-Taylor two-phase vortex flows and the test results showed high accuracy and highly correct tracking percent compared with the exact solution. An experiment of the bubbly jet flow was also conducted as a practical demonstration of the present method. As a result, it is confirmed from the simulation image examination and the experimental measurement that the proposed method shows a good performance in the measurement of bubble and particle phases.

  1. DS-777 Spatial Location of Gages with Total Flow and estimated Base Flow, for the Predevelopment Simulation Period for the Northern High Plains Groundwater-Flow Model in Parts of Colorado, Kansas, Nebraska, South Dakota, and Wyoming

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Baseflow is the portion of streamflow derived from groundwater flow. It is an important component of the groundwater budget, and can be estimated using known total...

  2. Measurement of separation performance and numerical analysis on flow field of different cyclone separators in parallel%差异旋风分离器并联性能测量及流场分析

    Institute of Scientific and Technical Information of China (English)

    陈建义; 高锐; 刘秀林; 李真发

    2016-01-01

    通过改变旋向和芯管直径,设计了3种差异旋风分离器,并按中心对称方式组成了3种并联方案:相同分离器、旋向差异分离器和芯管差异分离器并联。在冷态实验装置上,测量了单分离器和并联分离器的性能,并利用 FLUENT 软件分析了并联分离器的流场。结果表明,并联分离器的效率均高于单分离器,且效率-气速曲线未出现“驼峰”;与相同分离器并联相比,旋向交替变化时并联总压降较小,分离效率也更低,但各分离器流量分配均匀,未发现“窜流”现象;当芯管有差异时,并联总压降增大,各分离器进口流量分配不均匀,且进、出口流量平均相差6.0%,公共灰斗中存在“窜流”,旋流稳定性变差,效率降低。为了保证并联分离器的性能,应采用相同分离器对称并联的方式。%Three different cyclone separators with diameter of 300 mm, PV-1, PV-2 and PV-3, were designed by changing either the direction of rotation or the diameter of vortex finder. PV-1 differed from PV-2 in the direction of rotation, while PV-3 differed from PV-1 in the diameter of vortex finder. These cyclone separators were assembled center-symmetrically in three paralleled arrangements as assembly of same cyclones (Parallel-Ⅰ), assembly of various rotation (Parallel-Ⅱ) and assembly of various vortex finder (Parallel-Ⅲ). Parallel-Ⅰ was consisted of four PV-1 cell cyclones; Parallel-Ⅱ was consisted of two PV-1 cyclones and two PV-2 cyclones; Parallel-Ⅲ was consisted of two PV-1 cyclones and two PV-3 cyclones. Separation performances of single cyclone and cyclone assemblies were studied in a cold state experimental setup under a condition of solid loading at 5 g·m−3 and inlet velocities ranging from 14—26 m·s−1. The flow fields in these paralleled cyclones were simulated with FLUENT software. The results show that paralleled cyclones had higher efficiency than

  3. Hazard Identification of the Offshore Three-phase Separation Process Based on Multilevel Flow Modeling and HAZOP

    DEFF Research Database (Denmark)

    Wu, Jing; Zhang, Laibin; Lind, Morten;

    2013-01-01

    HAZOP studies are widely accepted in chemical and petroleum industries as the method for conducting process hazard analysis related to design, maintenance and operation of the systems. Different tools have been developed to automate HAZOP studies. In this paper, a HAZOP reasoning method based...... on function-oriented modeling, Multilevel Flow Modeling (MFM), is extended with function roles. A graphical MFM editor, which is combined with the reasoning capabilities of the MFM Workbench developed by DTU is applied to automate HAZOP studies. The method is proposed to support the “brain-storming” sessions...

  4. Active Control of Flow Separation on a High-Lift System with Slotted Flap at High Reynolds Number

    Science.gov (United States)

    Khodadoust, Abdollah; Washburn, Anthony

    2007-01-01

    The NASA Energy Efficient Transport (EET) airfoil was tested at NASA Langley's Low- Turbulence Pressure Tunnel (LTPT) to assess the effectiveness of distributed Active Flow Control (AFC) concepts on a high-lift system at flight scale Reynolds numbers for a medium-sized transport. The test results indicate presence of strong Reynolds number effects on the high-lift system with the AFC operational, implying the importance of flight-scale testing for implementation of such systems during design of future flight vehicles with AFC. This paper describes the wind tunnel test results obtained at the LTPT for the EET high-lift system for various AFC concepts examined on this airfoil.

  5. Determination of octan-1-ol-water partition coefficients by flow-injection extraction without phase separation

    Energy Technology Data Exchange (ETDEWEB)

    Kuban, V. (Royal Institute of Technology, Stockholm (Sweden). Department of Analytical Chemistry)

    1991-08-01

    Single-channel coaxial segments were used for the introduction of an aqueous or octan-1-ol solution of an organic substance directly into a continuous flow of the other solvent. The analytical signal was measured simultaneously on both aqueous and organic phase segments by an 'on-tube' fast-reading spectrophotometric detector (ca. 3 ms time resolution) and treated mathematically. The octan-1-ol-water phase signal ratio corresponds to the partition coefficient of the organic substances. The applicability of the method is demonstrated by the determination of partition coefficients of phenol, citric acid, acetylsalicylic acid and sodium salicylate. (author). 8 refs.; 3 figs.; 1 tab.

  6. Fluorescence in situ hybridization-flow cytometry-cell sorting-based method for separation and enrichment of type I and type II methanotroph populations.

    Science.gov (United States)

    Kalyuzhnaya, Marina G; Zabinsky, Rebecca; Bowerman, Sarah; Baker, David R; Lidstrom, Mary E; Chistoserdova, Ludmila

    2006-06-01

    A fluorescence in situ hybridization-flow cytometry (FISH/FC)-based method was optimized using artificial mixtures of pure cultures of methanotrophic bacteria. Traditional oligonucleotide probes targeting 16S rRNAs of type I (MG84/705 probe) and type II (MA450 probe) methanotrophs were labeled with fluorescein or Alexa fluor and used for FISH, followed by fluorescence-activated FC analysis and cell sorting (FACS). The method resulted in efficient separation of target cells (type I or type II methanotrophs) from the artificial mixtures. The method was then applied for detection and enrichment of type I and type II methanotroph populations from a natural sample, Lake Washington sediment. Cells were extracted from the sediment, fixed, and subjected to FISH/FC/FACS. The resulting subpopulations were analyzed by reverse transcriptase PCR surveys of 16S rRNA, pmoA (encoding a subunit of particulate methane monooxygenase), and fae (encoding formaldehyde-activating enzyme) genes. The functional gene analysis indicated specific separation of the type I and type II methanotroph populations. 16S rRNA gene analysis revealed that type I methanotrophs comprised 59% of the subpopulation separated using the type I-specific probe and that type II methanotrophs comprised 47.5% of the subpopulation separated using the type II-specific probe. Our data indicate that the FISH/FC/FACS protocol described can provide significant enrichment of microbial populations of interest from complex natural communities and that these can be used for genetic tests. We further tested the possibility of direct whole-genome amplification (WGA) from limited numbers of sorted cells, using artificial mixtures of microbes whose genome sequences are known. We demonstrated that efficient WGA can be achieved using 10(4) or more cells separated by 16S rRNA-specific FISH/FC/FACS, while fewer cells resulted in less specific WGA.

  7. Study on the Flow Injection Micro-Column Pre-Separation System Coupled With High Performance Liquid Chromatography for the Determination of Ecdysterone in Traditional Chinese Medicine

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A flow injection (FI) micro-column system coupled with high performance liquid chromatography (HPLC) was proposed for the pre-separation and determination of active organic component (ecdysterone) in traditional Chinese medicine, Loulu. The factors influencing separation performance were investigated and optimized. Under the optimal conditions, the contents of ecdysterone in Loulu were determined by HPLC system using MeOH-H20 (40 : 60,V/V) as the mobile phase at a flow rate of 1.0 mL/min. The calibration curve was linear in the range of 0. 5~100 mg/L of ecdysterone concentrations. The detection limit of the analyte was 0. 11 μmoi/L(3σ) with a precision of 0. 38% RSD (n=7, c=10. 0 mg/L). The average recovery of the method was 98.7%. The proposed method has been applied to determine ecdysterone in practical samples, and the determined values by both external standard method and standard addition method were in good agreement. Compared to the traditional solid extraction method, the system proposed has the advantages of simple procedure, good reproducibility, minimum volume requirement, reduction of matrix interference and iow contamination risk.

  8. Experimental investigation of droplet separation in a horizontal counter-current air/water stratified flow; Experimentelle Untersuchung der Tropfenabscheidung einer horizontalen, entgegengerichteten Wasser/Luft-Schichtenstroemung

    Energy Technology Data Exchange (ETDEWEB)

    Gabriel, Stephan Gerhard

    2015-07-01

    A stratified counter-current two-phase gas/liquid flow can occur in various technical systems. In the past investigations have mainly been motivated by the possible occurrence of these flows in accident scenarios of nuclear light water-reactors and in numerous applications in process engineering. However, the precise forecast of flow parameters, is still challenging, for instance due to their strong dependency on the geometric boundary conditions. A new approach which uses CFD methods (Computational Fluid Dynamics) promises a better understanding of the flow phenomena and simultaneously a higher scalability of the findings. RANS methods (Reynolds Averaged Navier Stokes) are preferred in order to compute industrial processes and geometries. A very deep understanding of the flow behavior and equation systems based on real physics are necessary preconditions to develop the equation system for a reliable RANS approach with predictive power. Therefore, local highly resolved, experimental data is needed in order to provide and validate the required turbulence and phase interaction models. The central objective of this work is to provide the data needed for the code development for these unsteady, turbulent and three-dimensional flows. Experiments were carried out at the WENKA facility (Water Entrainment Channel Karlsruhe) at the Karlsruhe Institute of Technology (KIT). The work consists of a detailed description of the test-facility including a new bended channel, the measurement techniques and the experimental results. The characterization of the new channel was done by flow maps. A high-speed imaging study gives an impression of the occurring flow regimes, and different flow phenomena like droplet separation. The velocity distributions as well as various turbulence values were investigated by particle image velocimetry (PIV). In the liquid phase fluorescent tracer-particles were used to suppress optical reflections from the phase surface (fluorescent PIV, FPIV

  9. Estimation of time-variable fast flow path chemical concentrations for application in tracer-based hydrograph separation analyses

    Science.gov (United States)

    Kronholm, Scott C.; Capel, Paul D.

    2016-09-01

    Mixing models are a commonly used method for hydrograph separation, but can be hindered by the subjective choice of the end-member tracer concentrations. This work tests a new variant of mixing model that uses high-frequency measures of two tracers and streamflow to separate total streamflow into water from slowflow and fastflow sources. The ratio between the concentrations of the two tracers is used to create a time-variable estimate of the concentration of each tracer in the fastflow end-member. Multiple synthetic data sets, and data from two hydrologically diverse streams, are used to test the performance and limitations of the new model (two-tracer ratio-based mixing model: TRaMM). When applied to the synthetic streams under many different scenarios, the TRaMM produces results that were reasonable approximations of the actual values of fastflow discharge (±0.1% of maximum fastflow) and fastflow tracer concentrations (±9.5% and ±16% of maximum fastflow nitrate concentration and specific conductance, respectively). With real stream data, the TRaMM produces high-frequency estimates of slowflow and fastflow discharge that align with expectations for each stream based on their respective hydrologic settings. The use of two tracers with the TRaMM provides an innovative and objective approach for estimating high-frequency fastflow concentrations and contributions of fastflow water to the stream. This provides useful information for tracking chemical movement to streams and allows for better selection and implementation of water quality management strategies.

  10. Isolation of the combined water content and salinity effects on ERT measurement to locate the preferential flow pathways in water repellent soils

    Science.gov (United States)

    Brindt, Naaran; Rahav, Matan; Furman, Alex; Wallach, Rony

    2016-04-01

    Electrical resistivity tomography (ERT) has been used for measuring the dynamics of water flow in soils without disturbing the soil, and recently for identifying the preferential flow pathways that are reported to develop in water repellent soils. Since electrical resistivity is affected mainly by soil saturation and salinity, and given that in many cases salinity in the root zone reaches high values, the isolation of spatial and temporal distribution of water content or salinity in the root zone from ERT scans is a challenge. A model for transient variation of soil water content and salinity within a well-mixed soil unit was developed in the frame of this challenge. The model aims to isolate the temporal changes in water content from subsequent ERT scans. The model assumes that four stages of water dynamics occur in the root zone during an irrigation cycle: 1) Soil water content decreases by evapotranspiration - no irrigation, 2) Irrigation with saline water begins, water content increases but remains below field capacity - negligible drainage, 3) Irrigation continues and drainage starts as the water content becomes higher than field capacity, and 4) Irrigation stops, water content is higher than field capacity, and water content decreases by drainage and evapotranspiration. These four stages restart when drainage stops and water content decreases solely by evapotranspiration. The model was solved analytically and successfully applied to a series of sequential ERT scans accomplished during and between subsequent irrigation events for a soil that was rendered hydrophobic by olive trees irrigated with saline water, and a soil in a citrus orchard that was rendered hydrophobic by prolonged effluent irrigation. The suggested model helps in distinguishing between the temporal changes in water content and salinity within a given soil volume, locating the preferential plow pathways, and tracking the spatial and temporal salinity variation within the root zone during and

  11. Location Privacy

    Science.gov (United States)

    Meng, Xiaofeng; Chen, Jidong

    With rapid development of sensor and wireless mobile devices, it is easy to access mobile users' location information anytime and anywhere. On one hand, LBS is becoming more and more valuable and important. On the other hand, location privacy issues raised by such applications have also gained more attention. However, due to the specificity of location information, traditional privacy-preserving techniques in data publishing cannot be used. In this chapter, we will introduce location privacy, and analyze the challenges of location privacy-preserving, and give a survey of existing work including the system architecture, location anonymity and query processing.

  12. Shark Skin Bristling as a Passive Mechanism for Separation Control

    Science.gov (United States)

    Wheelus, Jennifer; Lang, Amy; Jones, Emily

    2011-11-01

    The skin of fast-swimming sharks is proposed to have mechanisms to reduce drag and delay flow separation. The skin of fast-swimming and agile sharks is covered with small teeth-like denticles on the order of 0.2 mm. The shortfin mako is one of the fastest and most agile ocean predators creating the need to minimize its pressure drag by controlling flow separation. Biological studies of the shortfin mako skin have shown the passive bristling angle of their denticles to exceed 50 degrees in areas on the flank corresponding to the locations likely to experience separation first. It is proposed that reversing flow, as occurs at the onset of separation in a turbulent boundary layer, would activate denticle bristling and hinder local separation from leading to global separation over the shark. This study focuses on the denticle reaction to various reversed flow conditions using a pulsating jet. Mako shark skin was subjected to numerous reversed flow velocities to determine the bristling onset velocity. Digital Particle Image Velocimetry (DPIV) and digital video were used to determine the flow conditions and denticle behavior. The effect of reversed flow velocity on denticle bristling and its relation to separation control will be discussed. Research funded by NSF (award 0932352).

  13. Mathematical modeling of separated two-phase turbulent reactive flows using a filtered mass density function approach for large eddy simulation

    Science.gov (United States)

    Carrara, Mark David

    2006-04-01

    The overall objective of this dissertation is the development of a modeling and simulation approach for turbulent two-phase chemically reacting flows. A new full velocity-scalar filtered mass density function (FMDF) formulation for large eddy simulation (LES) of a separated two-phase flow is developed. In this formulation several terms require modeling that include important conditionally averaged phase-coupling terms (PCT). To close the PCT a new derivation of the local instantaneous two-phase equations is presented and important identities are derived relating the PCT to surface averages. The formulation is then applied for two particle laden flow cases and solved using a full particle based Monte-Carlo numerical solution procedure. The first case is a temporally developing counter-current mixing layer dilutely seeded with evaporating water droplets. Validation studies reveal excellent agreement of the full particle method to previous hybrid FDF studies and direct numerical simulations for single-phase flows. One-way coupled simulations reveal that the overall dispersion is maximized with unity Stokes number droplets. Two-way coupled simulations reveal the advantages of two FDF approaches where the subgrid variation of droplet properties are explicitly taken into account. Comparisons of the fully-coupled FDF approach are compared to more approximate means of determining phase-coupling based on filtered properties and local and compounded global errors are assessed. The second case considered is the combustion aluminum particles. A new mechanistic model for the ignition and combustion of aluminum particulate is developed that accounts for unsteady heating, melting, heterogeneous surface reactions (HSR) and quasi-steady burning. Results of this model agree well with experimental data for overall burn rates and ignition times. Two-phase simulations of aluminum particulate seeded mixing layer reveal the variations in flame radius resulting in local extinguishment

  14. A Geochemical Mass-Balance Method for Base-Flow Separation, Upper Hillsborough River Watershed, West-Central Florida, 2003-2005 and 2009

    Science.gov (United States)

    Kish, G.R.; Stringer, C.E.; Stewart, M.T.; Rains, M.C.; Torres, A.E.

    2010-01-01

    Geochemical mass-balance (GMB) and conductivity mass-balance (CMB) methods for hydrograph separation were used to determine the contribution of base flow to total stormflow at two sites in the upper Hillsborough River watershed in west-central Florida from 2003-2005 and at one site in 2009. The chemical and isotopic composition of streamflow and precipitation was measured during selected local and frontal low- and high-intensity storm events and compared to the geochemical and isotopic composition of groundwater. Input for the GMB method included cation, anion, and stable isotope concentrations of surface water and groundwater, whereas input for the CMB method included continuous or point-sample measurement of specific conductance. The surface water is a calcium-bicarbonate type water, which closely resembles groundwater geochemically, indicating that much of the surface water in the upper Hillsborough River basin is derived from local groundwater discharge. This discharge into the Hillsborough River at State Road 39 and at Hillsborough River State Park becomes diluted by precipitation and runoff during the wet season, but retains the calcium-bicarbonate characteristics of Upper Floridan aquifer water. Field conditions limited the application of the GMB method to low-intensity storms but the CMB method was applied to both low-intensity and high-intensity storms. The average contribution of base flow to total discharge for all storms ranged from 31 to 100 percent, whereas the contribution of base flow to total discharge during peak discharge periods ranged from less than 10 percent to 100 percent. Although calcium, magnesium, and silica were consistent markers of Upper Floridan aquifer chemistry, their use in calculating base flow by the GMB method was limited because the frequency of point data collected in this study was not sufficient to capture the complete hydrograph from pre-event base-flow to post-event base-flow concentrations. In this study, pre-event water

  15. Determination of 2-propanol in surface cleaning solutions used for copper continuous casting process by flow injection-spectrophotometric detection with on-line column separation.

    Science.gov (United States)

    Hayashibe, Yutaka; Tokuda, Masahiro; Takeya, Minoru

    2003-09-01

    A flow-injection system has been developed for the determination of 2-propanol in the surface cleaning solutions used in the copper continuous cast rod making system. Adsorption chromatography in nitric acid medium was used for the on-line separation of oily substances in the sample solution. Cerium(IV) diammonium nitrate was utilized as the chromogenic reagent for the spectrophotometric detection of 2-propanol. The system permits a throughput of one sample per hour for the oily sample, and of 12 samples per hour for the none-oily sample. The reproducibility has been proven to be satisfactory with a relative standard deviation of less than 6.0% (2.2%(V/V) 2-propanol level, n = 23). The detection limit is 0.01% (V/V).

  16. Library Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Carnegie Library of Pittsburgh locations including address, coordinates, phone number, square footage, and standard operating hours.

  17. Rapid isoelectric point determination in a miniaturized preparative separation using jet-dispensed optical pH sensors and micro free-flow electrophoresis.

    Science.gov (United States)

    Herzog, Christin; Beckert, Erik; Nagl, Stefan

    2014-10-01

    Herein, the fabrication, characterization, calibration, and application of integrated microfluidic platforms for fast isoelectric point (pI) determinations via free-flow electrophoresis with integrated inkjet-printed fluorescent pH sensor microstructures are presented. These devices allow one to determine the pI of a biomolecule from a sample mixture with moderately good precision and without addition of markers in typically less than 10 s total separation and analysis time. Polyhydroxyethyl methacrylate (pHEMA) hydrogels were covalently coupled with fluorescein and hydroxypyrene trisulfonic acid (HPTS)-based pH probes. These were piezoelectrically jet-dispensed onto acrylate-modified glass as pH sensor microarrays with a diameter of 300-600 μm and thicknesses of 0.4-2.4 μm with high spatial accuracy. Microchip fabrication and integration of these pH sensor arrays was realized by multistep liquid-phase photolithography from oligoethylene glycol precursors resulting in glass-based microfluidic free-flow isoelectric focusing (μFFIEF) chips with integrated pH observation capabilities. The microchips were characterized with regard to pH sensitivity, response times, photo-, and flow stability. Depending on the sensor matrix, they allowed IEF within a pH range of roughly 5.5-10.5 with good sensitivity and fast response times. These microchips were used for FFIEF of small molecule markers and several protein mixtures with simultaneous monitoring of local pH. This allowed the determination of their pI via multispectral imaging of protein and pH sensor fluorescence without addition of external markers. Obtained pI's were generally in good agreement with known data, demonstrating the applicability of the method for pI determination in micropreparative procedures within a time frame of a few seconds only.

  18. tance, Narrow Space, Single-Phase Flow, Double-Phase Flow Experirnents and analyses of the erosion of volute cyclone separators%蜗壳式旋风分离器的磨损实验和分析

    Institute of Scientific and Technical Information of China (English)

    魏耀东; 刘仁桓; 燕辉; 时铭显

    2001-01-01

    用模拟方法对蜗壳式旋风分离器器壁磨损进行了实验,探讨了旋风分离器的磨损机理,对蜗壳式旋风分离器器壁磨损最严重的3个部位:环形空间筒体60~135°(以入口处为0°)的局部区域、锥体部分的末端、灰斗锥体与料腿的连接附近的磨损特点进行了分析。实验结果表明,蜗壳式旋风分离器器壁磨损量与含尘气流的浓度是线性关系,与入口速度是三次方关系。在实验数据的基础上建立了磨损量计算的经验关系式。%The experiments were made for the erosion of the wall of the volute cyclone separators by using simulation,and the erosion mechanism of the volute cyclone separators was discussed.Three most serious erosion 1ocations i.e,the 60°~ 135°location of the annular cylinder(the inlet was 0°),the end of the cone body,and the connection between the hopper cone body and the dipleg,were analyzed.The experimental result shows that the relation betweet the wall erosion of the volute cyclone separators and the gas concentration with dust was linear, and the relation between the wall erosion of the volute cyclone separators and the speed at the inlet was cubic.

  19. The use of a polymer inclusion membrane in flow injection analysis for the on-line separation and determination of zinc.

    Science.gov (United States)

    Zhang, Lujia L; Cattrall, Robert W; Kolev, Spas D

    2011-06-15

    This paper reports the first use of a polymer inclusion membrane (PIM) for on-line separation in flow injection analysis (FIA) involving simultaneous extraction and back-extraction. The FIA system containing the PIM separation module was used for the determination of Zn(II) in aqueous samples in the presence of Mg(II), Ca(II), Cd(II), Co(II), Ni(II), Cu(II), and Fe(III). The Fe(III) and Cu(II) interferences were eliminated by off-line precipitation with phosphate and on-line complexation with chloride, respectively. The concentration of Zn(II) was determined spectrophotometrically using 4-(2-pyridylazo) resorcinol (PAR). The optimal composition of the PIM consisted of 40% (m/m) di(2-ethlyhexyl) phosphoric acid (D2EHPA) as carrier, 10% (m/m) dioctyl phthalate (DOP) as plasticizer and 50% (m/m) poly(vinyl chloride) (PVC) as the base polymer. The optimized FIA system was characterized by a linear calibration curve in the range from 1.0 to 30.0 mg L(-1) Zn(II), a detection limit of 0.05 mg L(-1) and a relative standard deviation of 3.4% with a sampling rate of 4h(-1). Reproducible results were obtained for 20 replicate injections over a 5h period which demonstrated a good membrane stability. The FIA system was applied to the determination of Zn(II) in pharmaceuticals and samples from the galvanizing industry and very good agreement with atomic absorption spectrometry was obtained.

  20. Segmentation method for touching pest images based on shape factor and separation points location%基于形状因子和分割点定位的粘连害虫图像分割方法

    Institute of Scientific and Technical Information of China (English)

    李文勇; 李明; 钱建平; 孙传恒; 杜尚丰; 陈梅香

    2015-01-01

    Image segmentation is the precondition of feature extraction and recognition. In order to improve segmentation accuracy of touching objects in pest identification and counting system, an image segmentation algorithm based on shape factor and separation point location was presented. In this method, a shape factor that was defined using area and perimeter of a region was used to be a parameter to justify whether the region was one touching region or not. In this paper, the threshold of shape factor was set to be 0.50. And then, if a region was a touching one, its contour was stripped layer by layer. In each contour, it was necessary to check whether a local segmentation point existed or not. There were two types of local segmentation points. The first type was a point that was found twice in one contour at the same time, whose traversal sequence number satisfied the determined threshold condition. The second type was one point that could be found in one contour with its four connected region points at the same time, and the difference between their traversal sequence numbers satisfied the same threshold condition. Once the local segmentation point was found, two separating points of this touching region were searched and located in its original contour. The search method was based on the shortest distance between the local segmentation and the background pixel points. At last, the segmentation lines were plotted between the local segmentation and the two separating points. In order to verify the validity of the proposed algorithm, three types of touching images, such as serial connection, loop connection and hybrid connection images were used. The results showed that the proposed method could locate the local segmentation points and separating points more accurately than the watershed method. In addition, the lab and field images were used to test reliability of the proposed method. In the lab experiment, 100 yellow peach moth (Conogethes punctiferalis

  1. The remarkable occurrence of large rainfall-induced debris flows at two different locations on July 12, 2008, Southern Sierra Nevada, CA, USA

    Science.gov (United States)

    DeGraff, J.V.; Wagner, D.L.; Gallegos, A.J.; DeRose, M.; Shannon, C.; Ellsworth, T.

    2011-01-01

    On July 12, 2008, two convective cells about 155 km apart produced a brief period of intense rainfall triggering large debris flows in the southern Sierra Nevada. The northernmost cell was centered over Oak Creek Canyon, an east-flowing drainage, and its tributaries near Independence, CA, USA. About 5:00 P.M., debris flows passed down the South Fork and North Fork of Oak Creek to merge into a large single feature whose passage affected the historic Mt. Whitney Fish hatchery and blocked California State Highway 395. At about the same time, the southernmost cell was largely centered over Erskine Creek, a main tributary of the west-flowing Kern River. Debris flows issued from several branches to coalesce into a large debris flow that passed along Erskine Creek, through the town of Lake Isabella, CA, USA and into the Kern River. It was observed reaching Lake Isabella about 6:30 P.M. Both debris flows caused significant disruption and damage to local communities. ?? 2011 Springer-Verlag.

  2. Preparation of low flow-resistant methacrylate-based monolithic stationary phases of different hydrophobicity and the application to rapid reversed-phase liquid chromatographic separation of alkylbenzenes at high flow rate and elevated temperature.

    Science.gov (United States)

    Ueki, Yuji; Umemura, Tomonari; Iwashita, Yoshikazu; Odake, Tamao; Haraguchi, Hiroki; Tsunoda, Kin-ichi

    2006-02-17

    Low flow-resistant alkyl methacrylate-based monolithic stationary phases of different hydrophobicity were constructed for reversed-phase capillary liquid chromatography by thermally initiated radical polymerization of respective methacrylate ester monomer with different alkyl chain (C2, C4, C6, C12, C18) and ethylene glycol dimethacrylate (EDMA) in a 250 microm i.d. fused silica capillary. The hydrophobicity was basically controlled by changing the length and/or the density of the alkyl-chain, while the composition and the ratio of porogenic solvent were adjusted to obtain highly permeable rigid monoliths with adequate column efficiency. Among the prepared monolithic stationary phases, C18-methacrylate monoliths polymerized from a binary porogenic solvent of isoamyl alcohol and 1,4-buthandiol exhibited the most promising performance in terms of hydraulic resistance and column efficiency. The pressure drops of 20-cm long monolithic columns were below approximately 0.4 MPa at a normal linear velocity of 1mm/s (a flow rate of 3 microL/min), and the numbers of theoretical plates for alkylbenzenes mostly exceeded 3000 plates/20 cm. The produced monolithic columns had good mechanical strength for high pressure and temperature, and could be properly operated even at a temperature of 80 degrees C and at a pressure of at least 33 MPa. At 80 degrees C, the theoretical plate numbers reached 6000 plates/20 cm because of the enhanced mass transfer. Due to the novel hydraulic resistance and mechanical strength, the separation time could be reduced 120-fold simply by raising the flow rate and column temperature.

  3. VARIATION FEATURES OF SOMALI CROSS-EQUATORIAL FLOW AND ITS IMPACT ON THE LOCATION OF THE SUBTROPICAL HIGH RIDGE FROM JULY TO SEPTEMBER

    Institute of Scientific and Technical Information of China (English)

    LIN Xin-bin; XU Jin-jing; WEN Zhen-zhi; HE Fen; CHI Yan-zhen

    2008-01-01

    The variation features of the cross-equatorial flow and its impact on the ridge position of the subtropical high have been analyzed in this paper. It is shown as follows. (1) The intensity of the Somalicross-equatorial flow is increasing in winter and summer in the past 44 years and the airflow of Northem Hemisphere exchanges more and more intensively with that of Southern Hemisphere. (2) The Somalicross-equatorial flow in May has the most impact on the ridge position of the subtropical high in the typhoon season, presenting a positive correlation. (3) The diagnosis is consistent with the real situation in 2005.

  4. Sequential Determination of Total Arsenic and Cadmium in Concentrated Cadmium Sulphate Solutions by Flow-Through Stripping Chronopotentiometry after Online Cation Exchanger Separation

    Directory of Open Access Journals (Sweden)

    Frantisek Cacho

    2012-01-01

    Full Text Available Flow-through stripping chronopotentiometry with a gold wire electrode was used for the determination of total arsenic and cadmium in cadmium sulphate solutions for cadmium production. The analysis is based on the online separation of arsenic as arsenate anion from cadmium cations by means of a cation exchanger. On measuring arsenate in the effluent, the trapped cadmium is eluted by sodium chloride solution and determined in a small segment of the effluent by making use of the same electrode. The elaborated protocol enables a full automatic measurement of both species in the same sample solution. The accuracy of the results was confirmed by atomic absorption spectrometry. The LOD and LOQ for Arsenic were found to be 0.9 μg dm-3 and 2.7 μg dm-3, respectively. A linear response range was observed in the concentration range of 1 to 300 μg dm-3 for sample volumes of 4 mL. The repeatability and reproducibility were found to be 2.9% and 5.2%, respectively. The linear response range for cadmium was found to be 0.5 to 60 g/L. The method was tested on samples from a cadmium production plant.

  5. Estimating ground water discharge by hydrograph separation.

    Science.gov (United States)

    Hannula, Steven R; Esposito, Kenneth J; Chermak, John A; Runnells, Donald D; Keith, David C; Hall, Larry E

    2003-01-01

    Iron Mountain is located in the West Shasta Mining District in California. An investigation of the generation of acid rock drainage and metals loading to Boulder Creek at Iron Mountain was conducted. As part of that investigation, a hydrograph separation technique was used to determine the contribution of ground water to total flow in Boulder Creek. During high-flow storm events in the winter months, peak flow in Boulder Creek can exceed 22.7 m3/sec, and comprises surface runoff, interflow, and ground water discharge. A hydrograph separation technique was used to estimate ground water discharge into Boulder Creek during high-flow conditions. Total ground water discharge to the creek approaches 0.31 m3/sec during the high-flow season. The hydrograph separation technique combined with an extensive field data set provided reasonable estimates of ground water discharge. These estimates are useful for other investigations, such as determining a corresponding metals load from the metal-rich ground water found at Iron Mountain and thus contributing to remedial alternatives.

  6. Numerical Simulation of Fluid Flow and Oil-Water Separation in Hydrocyclones%水力旋流分离器内流动和油水分离的数值模拟

    Institute of Scientific and Technical Information of China (English)

    陆耀军; 周力行

    2003-01-01

    The fluid flow and oil-water separation were simulated using a Reynolds stress transport equationmodel of turbulence in water flow and a stochastic model of oil droplet motion. Simulation results give the axialand tangential velocity components, the pressure and turbulence intensity distribution and droplet trajectories fora hydrocyclone of F type and a hydrocyclone proposed by the present authors. The flow field predictions are inqualitative agreement with the LDV measurements. The results show that the proposed hydrocyclone has betterperformance than the hydrocyclone of F type due to creating stronger centrifugal force and lower axial velocity.

  7. Acoustofluidic bacteria separation

    Science.gov (United States)

    Li, Sixing; Ma, Fen; Bachman, Hunter; Cameron, Craig E.; Zeng, Xiangqun; Huang, Tony Jun

    2017-01-01

    Bacterial separation from human blood samples can help with the identification of pathogenic bacteria for sepsis diagnosis. In this work, we report an acoustofluidic device for label-free bacterial separation from human blood samples. In particular, we exploit the acoustic radiation force generated from a tilted-angle standing surface acoustic wave (taSSAW) field to separate Escherichia coli from human blood cells based on their size difference. Flow cytometry analysis of the E. coli separated from red blood cells shows a purity of more than 96%. Moreover, the label-free electrochemical detection of the separated E. coli displays reduced non-specific signals due to the removal of blood cells. Our acoustofluidic bacterial separation platform has advantages such as label-free separation, high biocompatibility, flexibility, low cost, miniaturization, automation, and ease of in-line integration. The platform can be incorporated with an on-chip sensor to realize a point-of-care sepsis diagnostic device.

  8. Pore-scale simulation of fluid flow passing over a porously covered square cylinder located at the middle of a channel, using a hybrid MRT-LBM-FVM approach

    Science.gov (United States)

    Salimi, Mohammad Reza; Taeibi Rahni, Mohammad; Jam, Freydun

    2015-06-01

    A comprehensive study was performed to analyze the unsteady laminar flow characteristics around a porously covered, a fully porous, and a solid squared section cylinder located in the middle of a plane channel. In order to simulate fluid flow inside porous media and porous-fluid interface accurately (minimizing modeling error), the porous region was analyzed in pore scale, using LBM. Additionally, to minimize the LBM-related compressibility error through the porous region, a multi-block multiple relaxation time lattice Boltzmann method (MRT-LBM) was used. Also, to decrease CPU time, a Navier-Stokes flow solver, based on finite volume method and SIMPLE algorithm, was coupled with MRT-LBM to simulate flow around the porous obstacle. It should be noted that the flow inside the porous layer is in continuum regime, and hence, the no-slip boundary condition was used to treat the solid walls inside the porous media. In our simulations, we considered variations of porosity and Reynolds number ranging from 0.75 to 0.94 and from 60 to 240, respectively. The effects of porosity and Reynolds number on vortex pattern, mean drag coefficient, amplitude of lift coefficient, and Strouhal number were investigated. Comparison of our results with the ones obtained using Open FOAM, as well as published by others, shows the suitable accuracy of our computations. It is seen that at low Reynolds numbers or at low porosities, where the mean flow does not have large enough momentum to penetrate porous media, the resulting flow field and aerodynamic coefficients are relatively close for three different configurations used. However, as the flow Reynolds number or permeability increases, the mean flow penetrates easier into the porous media and thus provides different shedding characteristics and aerodynamic coefficients for different obstacle shapes.

  9. Research Progress of Separate Membrane for Vanadium Redox Flow Battery%钒液流电池用隔离膜研究进展

    Institute of Scientific and Technical Information of China (English)

    胡冰; 徐睿杰; 雷彩红; 石文强; 蔡启

    2013-01-01

    钒液流电池是近年来发展最为迅猛的储能电池之一.隔膜作为钒电池的重要组成部分直接关系到钒电池的转化储能效率和使用寿命.本文综述了近年来钒电池用隔离膜的发展现状.全氟磺酸质子交换膜(Nafion膜)作为当前使用最为广泛的隔膜,从传导机理、交换机理和表面涂覆、交联、复合等表面改性技术方面入手做了深入的研究,并对比分析了各种改性方法的优缺点.对磺化的特种工程塑料为主的非氟耐热型质子交换膜和功能化的聚烯烃隔膜在钒电池中的当前进展做了全面总结,并对钒液流电池用电池隔膜的发展方向做了展望.%Vanadium Redox Flow Batteries (VRB) is one of the most rapidly developed accumulation energy in recent years.Regard as the most important component,the separate membrane in VRB is directly related to the efficiency of transformation of energy storage and service life.In this paper,we give a brief introduction of the present situation of the VRB separate membrane.Nafion membrane as the most widely used membrane is discussed on the conduction mechanism,the exchange mechanism and the modification technologies,such as surface coating,crosslinking,surface recombination and so on.Then the advantages and disadvantages of various modification methods were analyzed by compared with each other.In addition,recent progresses about the non perfluorinated heat-resistant proton exchange membrane of sulfonated special engineering plastic membrane and the functionalization of polyolefin membrane are made a comprehensive summary.At last,we give the development trend of VRB in the future.

  10. Investigation of nonlinear turbulence models for separated supersonic flows%超声速分离流非线性湍流模式的研究

    Institute of Scientific and Technical Information of China (English)

    杨晓东; 马晖扬

    2002-01-01

    本文在低雷诺数k-ε两方程框架下,应用八个常见的非线性湍流模式,对两个激波/边界层相互作用诱导分离的超声速流动进行了研究.采用的非线性模式有:二阶模式(Wilcox & Rubesin (1980), Shih, Zhu & Lumley (1993), Shih, Zhu & Lumley (1995), Gatski & Speziale (1993))和三阶模式(Craft, Launder & Suga (1996), Lien & Leschziner (1996), Apsley & Leschziner (1998), Shih (1997)).两个超声速流动为:20°可压缩拐角绕流和轴对称尖顶拱-柱-裙组合体绕流.计算结果表明,对于激波边界层相互作用,在不做任何可压缩性修正的情况下,非线性模式并没有给出明显优于线性模式的结果.%Eight popular nonlinear turbulence models under low-Re k-ε framework have been tested and validated against experimental data of two supersonic flows with shock-wave/ boundary-layer interaction including separation. These models are: the nonlinear quadratic models ( Wilcox & Rubesin (1980), Shih, Zhu & Lumley (1993), Shih, Zhu & Lumley (1995), Gatski & Speziale (1993) ) and the nonlinear cubic models ( Craft, Launder & Suga (1996), Lien & Leschziner (1996), Apsley & Leschziner (1998), Shih (1997) ). The configurations consist of a 20°compression corner and an axisymmetric ogive-cylinder-flare. The computational results show that nonlinear models yield little improvement over linear models without any compressibility correction.

  11. Flow Separation and Numerical Simulation of Solid Rocket Motor Nozzle%固体火箭发动机喷管分离流动及其数值模拟

    Institute of Scientific and Technical Information of China (English)

    王晓辉; 于存贵

    2011-01-01

    Separation flow could be generated in a high-altitude nozzle, in order to study the flow separation on the nozzle performance. A three-dimensional numerical simulation about the separation flow in the high-altitude nozzle of a solid rocket motor (SRM) was conducted. The flow results with different inlet total pressure were obtained by utilizing CFX software. It shows that, due to the separation flow, there are some adverse effects for the stability and thermal protection of the nozzle. The simulation provides a reference of the design of the high-altitude nozzle, also a basis for further study.%大面积比喷管在火箭发动机工作过程中可能产生流动分离的问题,为研究喷管流动分离对喷管性能的影响,利用计算流体力学软件CFX对某固体火箭发动机大面积比喷管内燃气分离流动进行数值模拟.计算出喷管在几种不同入口总压情况下的流场参数分布,显示分离流动会改变燃气内流场流动参数分布,进而会对喷管推力稳定性和热防护性产生不利影响.该研究能为进一步研究大面积比喷管设计提供参考.

  12. Response of Stream Chemistry During Base Flow to Gradients of Urbanization in Selected Locations Across the Conterminous United States, 2002-04

    Science.gov (United States)

    Sprague, Lori A.; Harned, Douglas A.; Hall, David W.; Nowell, Lisa H.; Bauch, Nancy J.; Richards, Kevin D.

    2007-01-01

    During 2002-2004, the U.S. Geological Survey's National Water-Quality Assessment Program conducted a study to determine the effects of urbanization on stream water quality and aquatic communities in six environmentally heterogeneous areas of the conterminous United States--Atlanta, Georgia; Raleigh-Durham, North Carolina; Milwaukee-Green Bay, Wisconsin; Dallas-Fort Worth, Texas; Denver, Colorado; and Portland, Oregon. This report compares and contrasts the response of stream chemistry during base flow to urbanization in different environmental settings and examines the relation between the exceedance of water-quality benchmarks and the level of urbanization in these areas. Chemical characteristics studied included concentrations of nutrients, dissolved pesticides, suspended sediment, sulfate, and chloride in base flow. In three study areas where the background land cover in minimally urbanized basins was predominantly forested (Atlanta, Raleigh-Durham, and Portland), urban development was associated with increased concentrations of nitrogen and total herbicides in streams. In Portland, there was evidence of mixed agricultural and urban influences at sites with 20 to 50 percent urban land cover. In two study areas where agriculture was the predominant background land cover (Milwaukee-Green Bay and Dallas-Fort Worth), concentrations of nitrogen and herbicides were flat or decreasing as urbanization increased. In Denver, which had predominantly shrub/grass as background land cover, nitrogen concentrations were only weakly related to urbanization, and total herbicide concentrations did not show any clear pattern relative to land cover - perhaps because of extensive water management in the study area. In contrast, total insecticide concentrations increased with increasing urbanization in all six study areas, likely due to high use of insecticides in urban applications and, for some study areas, the proximity of urban land cover to the sampling sites. Phosphorus

  13. Experimental Investigations on the Interactions Between Liquids and Structures to Passively Control the Surface-Driven Capillary Flow in Microfluidic Lab-On Systems to Separate the Microparticles for Bioengineering Applications

    Science.gov (United States)

    Mukhopadhyay, Subhadeep

    In this research paper, total 246 individual microfluidic devices have been fabricated by maskless lithography, hot embossing lithography and direct bonding technique. The effect of surface area to volume ratio on the surface-driven capillary flow of different liquids has been experimentally investigated in these microfluidic devices fabricated by polymethylmethacrylate (PMMA). Also, the individual effects of liquid viscosity and surface wettability on the surface-driven capillary flow of different liquids are experimentally investigated. The polystyrene particles of 10μm diameters have been separated from the aqueous microparticle suspensions in the microfluidic lab-on-a-chip systems with 100% separation efficiency. Also, the polystyrene particles of 5μm diameters have been separated from a different set of aqueous microparticle suspensions in the microfluidic lab-on-a-chip systems with 100% separation efficiency. The individual designs of the microfluidic lab-on-a-chip systems are a novel approach in this research paper. The effect of surface area to volume ratio on the separation time is experimentally investigated as another novel approach of this research paper.

  14. Locations, values, and uncertainties of hydraulic-head observations for the steady-state, prepumped period of the transient ground-water flow model, Death Valley regional ground-water flow system, Nevada and California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This digital geospatial data set contains the locations, values, and uncertainties of 700 hydraulic-head observations used in the steady-state, prepumped period of...

  15. Electroextraction separation of dyestuffs

    Energy Technology Data Exchange (ETDEWEB)

    Luo, G.S.; Yu, M.J.; Jiang, W.B.; Zhu, S.L.; Dai, Y.Y. [Tsinghua Univ., Beijing (China). Dept. of Chemical Engineering

    1999-03-01

    Electroseparation technologies have prospects for significant growth well into the next century. Electroextraction, a coupled separation technique of solvent extraction with electrophoresis, was used to remove dyestuffs from their aqueous stream. A study on the characteristics of the separation technique was carried out with n-butanol/acid-chrom blue K/water and n-butanol/methyl blue/water as working systems. A continuous separation equipment was designed and sued in this work. The influences of two-phase flow, field strength, and concentration of the feed on the recovery of solute were studied. The results showed that much higher recovery of solute with less solvent consumption could be achieved by using this technique to remove dyes from their aqueous streams, especially for the separation of the dilute solution. When the field strength is increased, the recovery and mass flux increase. When the feed flow rate and the initial solute concentration are increased, the recovery decreases and the mass flux increases.

  16. Location-routing problems

    Energy Technology Data Exchange (ETDEWEB)

    Laporte, G.

    1987-01-01

    Location-routing problems involve simultaneously locating a number of facilities among candidate sites and establishing delivery routes to a set of users in such a way that the total system cost is minimized. This paper presents a survey of such problems. It includes some applications and examples of location-routing problems, a description of the main heuristics that have been developed for such problems, and reviews of various formulations and algorithms used in solving these problems. A more detailed review is given of exact algorithms for the vehicle routing problem, three-index vehicle flow formulations, and two-index vehicle flow formulations and algorithms for symmetrical and non-symmetrical problems. It is concluded that location-routing problem research is a fast-growing area, with most developments occurring over the past few years; however, research is relatively fragmented, often addresses problems which are too specific and contains several voids which have yet to be filled. A number of promising research areas are identified. 137 refs., 3 figs.

  17. Measurement of the location of a particle in three dimensions using Mie scattering theory and wave optics: Application to flow in a microscopic field of view

    Science.gov (United States)

    Das, Kamal Kanti

    In this research, we have sought to develop a technique for measuring three-dimensional flow fields in small fluid volumes seeded with small spherical particles using a high numerical aperture (NA) microscope. The technique relies upon the knowledge of how the light is scattered from the particles to accurately determine their three dimensional position. We have combined Mie scattering theory and wave optics to predict the scattered field from spherical particles in a fluid medium using high NA collection optics. The model uses Mie scattering theory to calculate the optical field distribution on the intermediate planar interface between glass and air and then adopts a ray approach to propagate the field to the entrance pupil of an imaging system. We do not use a paraxial (parabolic wavefront) approximation and, therefore, our approach is applicable to the modeling of imaging systems with high aperture objectives. We have verified our theoretical model by measuring the scattering from polystyrene spheres illuminated with partially coherent, Koehler illumination in a transmitted light microscope with a 0.5 NA objective. Good agreement between our model and the experiment was achieved. We also developed a non-paraxial transformation for the lens and a vectorial model for the electromagnetic fields collected by a high NA objective. The model was also to determine the three-dimensional microscale based upon the motion of small particles in a seeded fluid. Application to laminar flow in a sub-millimeter channel and a thin liquid film demonstrate the utility of the technique. Preliminary results show that a wavelet based denoising technique may be used to process the data without loss of resolution.

  18. Presentation of test cases TC-2A, TC-2B, TC-2C, TC-2D - Twodimensional, incompressible, wall flows with separation

    DEFF Research Database (Denmark)

    Scheel Larsen, Poul

    1988-01-01

    The four test cases comprise the backfacing step at high Re-number (TC-2A) and low Re-number (TC-2B), a low Re-number boundary layer flow past a thin obstacle, fence-on-wall (TC-2C), and a high Re-number developed channel flow past a squareobstacle (TC-2D). Geometry, test conditions and available...

  19. Flow-Interception Facility Location Problem under Competitive Conditions%竞争环境下截流设施选址问题

    Institute of Scientific and Technical Information of China (English)

    申瑞玲; 霍佳震

    2013-01-01

      We consider a facility location model for locating a set of new facilities that compete for customer demand with each other, as well as with some pre-existing facilities both belong to the entity and the competitor , to capture the maximum profit of the entity.According to Huff gravity model, the probability that a customer se-lects a certain facility is proportional to its attractiveness and inversely proportional to the distance to the facility , we suppose the facility construction cost is relevant to its attractiveness , the problem is formulated as a non-linear integer program and solved by greedy algorithm .The numerical analysis indicates that the model and the method are practical.%  研究企业新建设施时,市场上已有设施存在的情况下,使本企业总体利润最大的截流设施选址问题。在一般截留设施选址模型的基础上引入引力模型,消费者到某个设施接受服务的概率与偏离距离及设施的吸引力相关,同时设施的建设费用与设施吸引力正相关,建立非线性整数规划模型并使用贪婪算法进行求解。数值分析表明,该算法求解速度快,模型计算精度较高。

  20. High-performance liquid chromatographic separation of rolipram, bupivacaine and omeprazole using a tartardiamide-based stationary phase influence of flow rate and temperature on the enantioseparation.

    Science.gov (United States)

    da Silva Junior, Ivanildo José; Sartor, João Paulo; Rosa, Paulo César Pires; de Veredas, Vinícius; Barreto Júnior, Amaro Gomes; Santana, Cesar Costapinto

    2007-08-24

    Chromatographic separation of the chiral drugs rolipram, bupivacaine and omeprazole on a tartardiamide-based stationary phase commercially named Kromasil CHI-TBB is shown in this work. The effect of temperature on the chromatographic separation of the chiral drugs using the Kromasil CHI-TBB stationary phase was determined quantitatively so as to contribute toward the design for the racemic mixtures of the named compound by using chiral columns. A decrease in the retention and selectivity factors was observed, when the column temperature increased. Van't Hoff plots provided the thermodynamic data. The variation of the thermodynamic parameters enthalpy and entropy are clearly negative meaning that the separation is enthalpy controlled.