Análise estrutural de folhas de Eugenia uniflora L. (Myrtaceae coletadas em ambientes rural e urbano, SP, Brasil Leaf anatomy of Eugenia uniflora L. (Myrtaceae in urban and rural environments, São Paulo State, Brazil

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Edenise Segala Alves

2008-03-01

Full Text Available Objetivou-se com este estudo verificar se plantas de Eugenia uniflora que crescem na cidade de São Paulo, diferem quanto à estrutura foliar, de exemplares encontrados em área rural, isenta de poluentes aéreos urbanos. Foram avaliadas, comparativamente, as dimensões da folha e, em microscopia de luz, a espessura dos tecidos foliares, a densidade de estômatos e de cristais da espécie, coletada em área rural e em dois pontos da cidade de São Paulo: canteiro central da Avenida dos Bandeirantes, com tráfego veicular intenso, portanto com alta carga de poluentes primários, e no Parque Estadual das Fontes do Ipiranga (PEFI submetido a altas concentrações de poluentes secundários. Buscaram-se variações que possam ser decorrentes da poluição urbana. As folhas coletadas no meio urbano mostraram menores dimensões, menor espessura do mesofilo, maior densidade estomática e maior quantidade de cristais. A espessura do parênquima lacunoso sofreu redução, quando se comparam as plantas do ambiente rural e urbano; observou-se a menor espessura nas folhas submetidas a poluentes secundários. Não foram observadas variações qualitativas entre as folhas dos três locais avaliados. Considerando que folhas coletadas no meio urbano variaram menos entre si, quando comparadas àquelas de área rural, acredita-se que a poluição aérea da cidade possa ser responsável, pelo menos em parte, pelas variações observadas. Exposições padronizadas, em ambiente monitorado, devem ser realizadas para comprovar tal hipótese.The aim of this study was to compare leaves of Eugenia uniflora from the city of São Paulo with leaves of plants from a rural site. Leaf size, tissue thickness, and stomatal and crystal density of E. uniflora growing at two sites in the city of São Paulo were investigated by light microscopy and compared with samples from a rural area. The level and types of air pollutants varied at the urban sites. Primary pollutants were present

Potentiation of antibiotic activity by Eugenia uniflora and Eugenia jambolanum.

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Coutinho, Henrique D M; Costa, José G M; Falcão-Silva, Vivyanne S; Siqueira-Júnior, José P; Lima, Edeltrudes O

2010-08-01

This is the first report about the modifying antibiotic activity of Eugenia uniflora L. and Eugenia jambolanum L. In this study the ethanol extract of E. uniflora and E. jambolanum was tested for their antimicrobial activity against strains of Escherichia coli. The growth of the two strains of E. coli bacteria tested was not inhibited in a clinically relevant form by the extract. The minimal inhibitory concentration was >or=1,024 microg/mL for both strains of E. coli assayed. Synergism between this extract and gentamicin was demonstrated. In the same extract synergism was observed between chlorpromazine and kanamycin and between amikacin and tobramycin, indicating the involvement of an efflux system in the resistance to these aminoglycosides. It is therefore suggested that extracts from E. uniflora L. and E. jambolanum L. could be used as a source of plant-derived natural products with modifying antibiotic activity to gentamicin.

(Eugenia dysenterica DC.) coated with chitosan and associated

African Journals Online (AJOL)

Aghomotsegin

2015-06-17

Jun 17, 2015 ... DOI: 10.5897/AJB2015.14646. Article Number: DB0746353710 ... In search of new technologies to preserve the quality of cagaita fruits, structural ... deformation of samples using a cylindrical acrylic plate (model. TA3/1000) at ...

Circumscription and synopsis of Eugenia section Speciosae Bünger & Mazine (Myrtaceae)

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de Oliveira Bünger, Mariana; Mazine, Fiorella Fernanda; Lucas, Eve J.; Stehmann, João Renato

2016-01-01

Abstract A new section of Eugenia (Myrtaceae) is described, segregate from Eugenia sect. Phyllocalyx. Phylogenetic studies suggest that Eugenia sect. Phyllocalyx as traditionally delimited is paraphyletic. To maintain the monophyly of each of the sections in Eugenia s.l., we herein opt to circumscribe a new section and recognize six taxa in sect. Speciosae, which has a distribution mostly in southeastern Brazil and northern South America. Nomenclatural notes are made and a taxonomic key is provided for the species of the section. PMID:27081351

Frugivoria por aves em Eugenia uniflora L. (Myrtaceae em ambientes antropizados na região de Sorocaba–SP. Frugivory by birds in Eugenia uniflora L. (Myrtaceae at anthropic environment in Sorocaba–SP region

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Nayara Francine LAMBERTI

2012-12-01

Full Text Available As aves, assim como toda fauna consumidora de frutos possuem grande importância no processo de dispersão de sementes. O declínio de suas populações pode gerar consequências para a reprodução e para o crescimento populacional dessas plantas zoocóricas, se a dispersão não for adequada ou se a quantidade de sementes dispersas for insuficiente. A espécie Eugenia uniflora (Myrtaceae, nativa do Brasil, é popularmente conhecida como pitangueira. Possui frutos globosos e sulcados, apresentando cores brilhantes desde o laranja, até vermelho ou preto, com polpa carnosa e agridoce, normalmente com umaa duas sementes. O presente estudo caracterizou as espécies de aves consumidoras dos frutos de E. uniflora, em dois ambientes da região de Sorocaba, Estado de São Paulo. O estudo foi realizado entre fevereiro e setembro de 2012 com seis indivíduos de E. uniflora, sendo três em ambiente altamente antropizado, e três em área menos antropizada. A avifauna foi amostrada por meio de observações focais, considerando sua riqueza, abundância relativa, frequência de visitas, comportamento e diversidade. O padrão fenológico de E. uniflora também foi descrito, assim como a quantidade de frutos produzidos. Foram realizadas 116 horasde observação focal, durante as quais foram registradas 185 visitas de aves pertencentes àsfamílias Thamnophilidae, Vireonidae, Turdidae, Thraupidae consumindo seus frutos. Os resultadosobtidos indicam que áreas menos antropizadas, mais arborizadas e com fragmentos mais próximos, como a área urbana de Araçoiaba, apresentam maior riqueza, abundância e diversidade de aves consumidoras de E. uniflora, quando comparada com a área urbana de Sorocaba. The birds, as all frugivorous animals, have great importance to seed dispersion process. Populational decline can generate consequences for the reproduction and population growth of many zoochoric plants if the dispersion is not adequate or if the amount of

Drying and storage of Eugenia involucrata DC. seeds Secagem e armazenamento de sementes de Eugenia involucrata DC.

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Angela Maria Maluf

2003-01-01

Full Text Available The physiological quality of seeds of native species is important to produce healthy saplings and therefore guarantee the success of programs to recover disturbed vegetation. This reinforces the necessity for investigating the physiological quality of those seeds. To evaluate the effects of different drying rates on the germination, moisture content and storability of Eugenia involucrata diaspores, mature fruits collected at Mogi Guaçu, SP, Brazil had their epi- and mesocarps removed by washing and were dried at 30, 40 or 50ºC until their water content was reduced from 57% (fresh diaspores to 13% (final drying, totaling six drying levels. In a second experiment, diaspores had their moisture content reduced from 57% to 49%, at 30ºC, totaling six drying levels (0h, 1h, 2h, 3h, 4h and 5h, and were kept for 180 days in plastic bags under cold storage. The drying rate had no effect on tolerance to desiccation by E. involucrata diaspores; water contents lower than 51% decreased both germinability and storability. Diaspores can be stored for up to 180 days as long as their water content is reduced to 53% and they are kept inside plastic bags under cold storage.O uso de sementes de espécies nativas de alta qualidade é fundamental nos programas de recomposição vegetal, o que fortalece a necessidade de se investigar o potencial fisiológico das mesmas. Esta pesquisa objetivou avaliar os efeitos da velocidade de secagem dos diásporos de Eugenia involucrata sobre a sua germinação e vigor, bem como as relações entre teor de água e capacidade de armazenamento. Foram colhidos frutos maduros em pomar instalado em Mogi Guaçu, SP (22º15-16'S, 47º8-12'W, que tiveram seu epicarpo e mesocarpo removidos por lavagem. A seguir, os diásporos (semente + endocarpo foram submetidos a secagem controlada a 30, 40 e 50ºC, com reduções progressivas do teor de água inicial de 57% para até 13%, obtendo-se seis níveis de secagem em cada temperatura. Em um

PEMANFAATAN DAUN SALAM (Eugenia polyantha SEBAGAI OBAT HERBAL DAN REMPAH PENYEDAP MAKANAN

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Kun Harismah

2017-02-01

Full Text Available Eugenia polyantha, one of Indonesian medicine plant, often used in society as traditional medicine and also Indonesian culinary additives. Daun salam is called Indonesian bay-leaf, the leaves contain only tiny amounts of an essential oil 0.2%. As main components, eugenol, methyl chavicol, and citral have been identified. Thus application to used of Eugenia polyantha have done. The aim of the present work was the application of Eugenia polyantha for food and health in the community area of Keraton Surakarta. The results indicated that Eugenia polyantha is of particular interest, since it may be an alternative way to carry out of food flavor simultanous as herbal medicine.

On the concept of eugenics: preliminaries to a critical appraisal Sobre o conceito de eugenia: preliminares à uma avaliação crítica

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Demetrio Neri

1999-01-01

Full Text Available This paper's main issue is linked to what can be foreseen as the increasing capability of medical genetics to modify the genetic composition of the human species through direct interventions in the human genome for medical and non-medical purposes, i.e., the 'risk' of a resurgence of eugenics. In current discussions on the topic (briefly presented in the first section, the 'phantom of eugenics' is raised several times, but there is a great deal of confusion on what counts as eugenics, partly because of broad conceptual disagreement over the notion itself. Furthermore, according to some scholars there is no hope of overcoming this unsatisfactory conceptual uncertainty. Partly challenging this opinion, the second and third sections of this paper attempt to identify some basic features which could be seen as intrinsically linked to the notion of eugenics, with the aim of reducing the range of conceptual disagreement as a preliminary step in bringing into focus what exactly is wrong with practicing eugenics. The subsequent sections deal with the substantive issue of whether or not to practice eugenics from the point of view of the interest of future generations in the human species' genetic composition. The main moral arguments for and against eugenics are examined from the point of view of our obligations towards future generations, and the conclusion is in favor of a cautious 'open-door' position.O ponto principal desse artigo está vinculado ao que pode ser antevisto como a crescente capacidade da genética médica de modificar a espécie humana por meio de intervenções diretas no genoma humano com propósitos médicos e não médicos, isto é, o risco do ressurgimento da eugenia. Nas atuais discussões sobre o tópico, o fantasma da eugenia é levantado diversas vezes, mas há grande confusão no que se refere ao que é considerado como eugenia, particularmente em razão da ampla discordância sobre a noção em si. Procura-se identificar

Evaluation of Antioxidant, Immunomodulatory, and Cytotoxic Action of Fractions from Eugenia uniflora L. and Eugenia malaccensis L.: Correlation with Polyphenol and Flavanoid Content

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Figueir?a, Evellyne de Oliveira; Nascimento da Silva, Lu?s Cl?udio; de Melo, Cristiane Moutinho Lagos; Neves, Juliana Kelle de Andrade Lemoine; da Silva, Nic?cio Henrique; Pereira, Val?ria R?go Alves; Correia, Maria Tereza dos Santos

2013-01-01

An increasing number of biological activities presented by medicinal plants has been investigated over the years, and they are used in the search for new substances with lower side effects. Eugenia uniflora L. and Eugenia malaccensis L. (Myrtaceae) have many folk uses in various countries. This current study was designed to quantify the polyphenols and flavonoids contents and evaluate the immunomodulatory, antioxidant, and cytotoxic potentials of fractions from E. uniflora L. and E. malaccens...

Hepatoprotective activity of Eugenia jambolana Lam. in carbon tetrachloride treated rats

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Sisodia, S.S.; Bhatnagar, M.

2009-01-01

Objective: To estimate the hepatoprotective effects of the methanolic seed extract of Eugenia jambolana Lam. (Myrtaceae), in Wistar albino rats treated with carbon tetrachloride (CCl4). Materials and Methods: Liver damage in rats treated with CCl4 (1ml/kg/Bw, administered subcutaneously, on alternate days for one week) was studied by assessing parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), acid phosphatase (ACP) and bilirubin (total and direct). The effect of co-administration of Eugenia jambolana Lam. (doses 100, 200 and 400 mg/kg p. o.) on the above parameters was investigated. These biochemical observations were supplemented by weight and histological examination of liver sections. Liv.52® was used as positive control. Data were analyzed by one way ANOVA, followed by Scheff's/Dunnett's test. Results: Administration of Eugenia jambolana Lam. (doses 100, 200 and 400 mg/kg p. o.) significantly prevented carbon tetrachloride induced elevation of serum SGOT, SGPT, ALP, ACP and bilirubin (total and direct) level. Histological examination of the liver section revealed hepatic regeneration, after administration of various doses of Eugenia jambolana Lam. The results were comparable to that of Liv.52®. Conclusion: The study suggests preventive action of Eugenia jambolana Lam. in carbon tetrachloride induced liver toxicity. Hepatic cell regeneration process was dose dependent. PMID:20177577

Involvement of monoaminergic systems in the antidepressant-like effect of Eugenia brasiliensis Lam. (Myrtaceae) in the tail suspension test in mice.

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Colla, André R S; Machado, Daniele G; Bettio, Luis E B; Colla, Guilherme; Magina, Michele D A; Brighente, Inês M C; Rodrigues, Ana Lúcia S

2012-09-28

Several species of Eugenia L. are used in folk medicine for the treatment of various diseases. Eugenia brasiliensis is used for the treatment of inflammatory diseases, whereas Eugenia. uniflora is used for the treatment of symptoms related to depression and mood disorders, and is used in Brazil by the Guarani Indians as a tonic stimulant. To investigate the antidepressant-like effect of hydroalcoholic extracts of different plant species of genus Eugenia and to characterize the participation of the monoaminergic systems in the mechanism of action of the specie that afforded the most prominent antidepressant-like efficacy. In the first set of experiments, the effects of hydroalcoholic extracts of Eugenia beaurepaireana, Eugenia brasiliensis, Eugenia catharinae, Eugenia umbelliflora and Eugenia uniflora and the antidepressant fluoxetine (positive control) administered acutely by p.o. route were evaluated in the tail suspension test (TST) and locomotor activity was assessed in the open-field test in mice. In the second set of experiments, the involvement of the monoaminergic systems in the antidepressant-like activity of Eugenia brasiliensis was evaluated by treating mice with several pharmacological agonists and antagonists. The effects of the combined administration of sub-effective doses of Eugenia brasiliensis and the antidepressants fluoxetine, imipramine and bupropion were also evaluated. The administration of the extracts from Eugenia brasiliensis, Eugenia catharinae and Eugenia umbelliflora, but not Eugenia beaurepaireana and Eugenia uniflora, exerted a significant antidepressant-like effect, without altering locomotor activity. The behavioral profile was similar to fluoxetine. Pre-treatment of mice with ketanserin, haloperidol, SCH23390, sulpiride, prazosin and yohimbine prevented the reduction of immobility time induced by Eugenia brasiliensis. Treatment with sub-effective doses of WAY100635, SKF38393, apomorphine, phenylephrine, but not clonidine, combined

Wound healing activity and chemical standardization of Eugenia pruniformis Cambess

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Ricardo Diego Duarte Galhardo de Albuquerque; Jamila Alessandra Perini; Daniel Escorsim Machado; Thaís Angeli-Gamba; Ricardo dos Santos Esteves; Marcelo Guerra Santos; Adriana Passos Oliveira; Leandro Rocha

2016-01-01

Background: Eugenia pruniformis is an endemic species from Brazil. Eugenia genus has flavonoids as one of the remarkable chemical classes which are related to the improvement of the healing process. Aims: To evaluate of wound healing activity of E. pruniformis leaves and to identify and quantify its main flavonoids compounds. Materials And Methods: Wound excision model in rats was used to verify the hydroethanolic and ethyl acetate extracts potential. The animals were divided in four groups o...

Bioactive Compounds Found in Brazilian Cerrado Fruits.

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Bailão, Elisa Flávia Luiz Cardoso; Devilla, Ivano Alessandro; da Conceição, Edemilson Cardoso; Borges, Leonardo Luiz

2015-10-09

Functional foods include any natural product that presents health-promoting effects, thereby reducing the risk of chronic diseases. Cerrado fruits are considered a source of bioactive substances, mainly phenolic compounds, making them important functional foods. Despite this, the losses of natural vegetation in the Cerrado are progressive. Hence, the knowledge propagation about the importance of the species found in Cerrado could contribute to the preservation of this biome. This review provides information about Cerrado fruits and highlights the structures and pharmacologic potential of functional compounds found in these fruits. Compounds detected in Caryocar brasiliense Camb. (pequi), Dipteryx alata Vog. (baru), Eugenia dysenterica DC. (cagaita), Eugenia uniflora L. (pitanga), Genipa americana L. (jenipapo), Hancornia speciosa Gomes (mangaba), Mauritia flexuosa L.f. (buriti), Myrciaria cauliflora (DC) Berg (jabuticaba), Psidium guajava L. (goiaba), Psidium spp. (araçá), Solanum lycocarpum St. Hill (lobeira), Spondias mombin L. (cajá), Annona crassiflora Mart. (araticum), among others are reported here.

Bioactive Compounds Found in Brazilian Cerrado Fruits

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Elisa Flávia Luiz Cardoso Bailão

2015-10-01

Full Text Available Functional foods include any natural product that presents health-promoting effects, thereby reducing the risk of chronic diseases. Cerrado fruits are considered a source of bioactive substances, mainly phenolic compounds, making them important functional foods. Despite this, the losses of natural vegetation in the Cerrado are progressive. Hence, the knowledge propagation about the importance of the species found in Cerrado could contribute to the preservation of this biome. This review provides information about Cerrado fruits and highlights the structures and pharmacologic potential of functional compounds found in these fruits. Compounds detected in Caryocar brasiliense Camb. (pequi, Dipteryx alata Vog. (baru, Eugenia dysenterica DC. (cagaita, Eugenia uniflora L. (pitanga, Genipa americana L. (jenipapo, Hancornia speciosa Gomes (mangaba, Mauritia flexuosa L.f. (buriti, Myrciaria cauliflora (DC Berg (jabuticaba, Psidium guajava L. (goiaba, Psidium spp. (araçá, Solanum lycocarpum St. Hill (lobeira, Spondias mombin L. (cajá, Annona crassiflora Mart. (araticum, among others are reported here.

Photoelectrochemical solar cell using extract of Eugenia jambolana Lam as a natural sensitizer

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Christian G. Garcia

2003-06-01

Full Text Available The extract of Jambolão (java plum, Eugenia jambolana Lam, was used as a natural sensitizer of a wide band-gap semiconductor (TiO2 in photoelectrochemical solar cells. The natural dye, adsorbed onto the semiconductor surface, absorbs visible light and promotes electron transfer across the dye/semiconductor interface. Photogenerated current and voltage as high as 2.3 mA and 711 mV, respectively, were obtained and effective conversion of visible light into electricity was achieved. The use of a natural product as the semiconductor sensitizer enables a faster and simpler production of cheaper and environmentally friendly solar cells.O extrato de Jambolão, Eugenia jambolana Lam, foi utilizado como um sensibilizador natural de um semicondutor com separação grande de bandas (TiO2 nas células solares fotoeletroquímica. O corante natural, adsorvido na superfície do semicondutor, absorve luz visível e promove transferência eletrônica na interface corante/semicondutor. A corrente e o potencial fotogerados tão altos como 2,3 mA e 711 mV, respectivamente, foram obtidos observando-se conversão eficiente de luz visível em eletricidade. O uso de um produto natural como o sensibilizador de semicondutor possibilita uma produção mais rápida e simples de células solares mais baratas que não agridem o meio ambiente.

Aspectos agronómicos sobre el cultivo del arazá (eugenia stipitata me vaugh Frutal promisorio de la amazonia colombiana

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Quevedo Garcia Enrique

1995-06-01

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En este trabajo se presenta un estudio del manejo agronómico del cultivo del arazá <em>(Eugenia slipitataem> Mc. Vaugh en la Amazonia colombiana y una revisión del manejo del arazá en la Amazonia del Brasil y Perú. Se concluye que esta es una especie en vía de domesticación que puede ser cultivada en otras regiones de Colombia y que podría ser un sustituto para los cultivos ilícitos. Si somos hábiles en logra establecer el cultivo del<em> em>arazá. En reglones con características ecológicas adecuadas para esta especie en corto tiempo, obtendremos un fruto para el consumo humano y su usa extensivo en Colombia.

O abajurú (Chrysobalanus icaco L. e Eugenia rotundifolia Casar.) comercializado na cidade do Rio de Janeiro, Brasil

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Silva,Inês Machline; Peixoto,Ariane Luna

2009-01-01

Este artigo analisa as prováveis razões de introdução e comercialização de uma espécie de uso medicinal em um mercado popular urbano na cidade do Rio de Janeiro, Brasil - o Mercado de Madureira. Durante os anos de 2005 e 2006 aplicaram-se entrevistas semi-estruturadas a 15 erveiros obtendo-se o freelist das espécies consideradas como mais comercializadas (97) a partir do qual se calculou o índice de saliência, que para o abajurú (Eugenia rotundifolia Casar), foi elevado. A espécie conhecida n...

Leaf blight and defoliation of Eugenia spp. caused by Cylindrocladium candelabrum and C. spathiphylli in Brazil

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Luiz Sebastião Poltronieri

2011-06-01

Full Text Available Leaf blight and defoliation of Eugenia stipitata Mc Vaugh and Eugenia patrisii Vahl, caused respectively by Cylindrocladium candelabrum (Calonectria scoparia and C. spathiphylli (Calonectria spathiphylli are reported in the state of Pará, Brazil. On both host species, the disease is characterized by dark brown lesions of different sizes and shapes. A whitish bright sporulation, resembling Cylindrocladium is observed on the necrotic lesions by using a stereomycroscope or a pocket lense (10-20 X. Under favorable conditions and depending on the level of infection, intense premature tree defoliation may also be found.Although the conidial germination and mycelial growth were higher at 25ºC for both species, C. candelabrum was more sensitive to the variation of temperature (10, 20, 30 and 40 ºC than C. spathiphylli. This is the first report of C. candelabrum and C. spathiphylli on Eugenia stipitata (araçá-boi and on Eugenia patrisii (ubaia-da-amazônia, respectively in Brazil.

Floral heterochrony promotes flexibility of reproductive strategies in the morphologically homogeneous genus Eugenia (Myrtaceae).

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Vasconcelos, Thais N C; Lucas, Eve J; Faria, Jair E Q; Prenner, Gerhard

2018-01-25

Comparative floral ontogeny represents a valuable tool to understand angiosperm evolution. Such an approach may elucidate subtle changes in development that discretely modify floral architecture and underlie reproductive lability in groups with superficial homogeneous morphology. This study presents a comparative survey of floral development in Eugenia (Myrtaceae), one of the largest genera of angiosperms, and shows how previously undocumented ontogenetic trends help to explain the evolution of its megadiversity in contrast to its apparent flower uniformity. Using scanning electron microscopy, selected steps of the floral ontogeny of a model species (Eugenia punicifolia) are described and compared with 20 further species representing all ten major clades in the Eugenia phylogenetic tree. Additional floral trait data are contrasted for correlation analysis and character reconstructions performed against the Myrtaceae phylogenetic tree. Eugenia flowers show similar organ arrangement patterns: radially symmetrical, (most commonly) tetramerous flowers with variable numbers of stamens and ovules. Despite a similar general organization, heterochrony is evident from size differences between tissues and structures at similar developmental stages. These differences underlie variable levels of investment in protection, subtle modifications to symmetry, herkogamic effects and independent androecium and gynoecium variation, producing a wide spectrum of floral display and contributing to fluctuations in fitness. During Eugenia's bud development, the hypanthium (as defined here) is completely covered by stamen primordia, unusual in other Myrtaceae. This is the likely plesiomorphic state for Myrteae and may have represented a key evolutionary novelty in the tribe. Floral evolution in Eugenia depends on heterochronic patterns rather than changes in complexity to promote flexibility in floral strategies. The successful early establishment of Myrteae, previously mainly linked to the

Maturação fisiológica e coleta de sementes de Eugenia uniflora L. (Pitanga, Santa Maria, RS.

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Angela Luciana de Avila

2009-09-01

Full Text Available Normal 0 21 MicrosoftInternetExplorer4 Este estudo teve como objetivo acompanhar a maturação fisiológica, seus parâmetros indicadores e identificar o momento adequado para a coleta de sementes de Eugenia uniflora, avaliando também a incidência de fungos em frutos e sementes. A pesquisa foi realizada de agosto a outubro de 2004 em Santa Maria, RS. A coleta dos frutos e sementes ocorreu semanalmente, partindo de três árvores matrizes, com início aos 7 dias após a antese (DAA e término aos 77 DAA. As variáveis observadas foram: altura, diâmetro, peso verde e sanidade de frutos e sementes e teor de umidade, massa seca e germinação das sementes. Identificou-se que a época adequada para a coleta das sementes, no período e local de estudo, ocorreu entre 56 e 63 DAA, quando os frutos apresentavam coloração vermelha e as sementes elevada germinação. Nesse período, o tamanho e o peso de frutos e sementes atingiram seus valores máximos e o teor de umidade e matéria seca das sementes mostraram tendência à estabilização. Os gêneros fúngicos Cladosporium sp. e Alternaria sp.  apresentaram as maiores taxas de incidência nos frutos e nas sementes e podem influenciar o armazenamento e a germinação das sementes de mudas de Eugenia uniflora.

Atividade inseticida de Eugenia uniflora L. e Melia azedarach L. sobre Atta laevigata Smith

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Paulo Henrique Jung

Full Text Available As formigas cortadeiras estão entre os principais insetos pragas das florestas plantadas brasileiras, causando danos econômicos desde a implantação até a sua colheita. Em razão das questões ambientais e da exigência da certificação florestal, métodos alternativos estão sendo desenvolvidos para o controle do gênero Atta. Neste sentido, o objetivo deste trabalho foi avaliar a atividade inseticida de pitangueira (Eugenia uniflora L. e cinamomo (Melia azedarach L. sobre soldados de Atta laevigatta Smith. Para tal, a partir de folhas, foram obtidos extratos pelos processos de decocção, infusão, maceração, extrato alcoólico e extração de óleo essencial, em diferentes concentrações. O óleo essencial de E. uniflora, nas concentrações de 1,25, 2,5 e 5%, apresentou potencial inseticida sobre soldados de A. laevigata, quando comparado às demais formas de obtenção. O extrato alcoólico de M. azedarach, a 10%, também apresentou potencial inseticida, podendo estes extratos serem testados em práticas de campo.

Atividade antioxidante de frutas do cerrado Antioxidant activity of cerrado fruits

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Roberta Roesler

2007-03-01

Full Text Available Annona crassiflora (araticum, Solanum lycocarpum (lobeira, Eugenia dysenterica (cagaita, Caryocar brasilense (pequi e Swartzia langsdorfii (banha de galinha são frutas do bioma cerrado, conhecidas e consumidas principalmente por populações nativas dessa região. Nesse estudo, as diferentes frações dos frutos acima descritos (polpa, semente e casca foram avaliadas por meio de extratos aquosos e etanólicos. Alguns extratos mostraram altíssimos conteúdos de compostos fenólicos e foram escolhidos para avaliação do potencial em seqüestrar radicais livres por meio do modelo 2,2 difenil-1-picril hidrazil (DPPH. Os melhores resultados foram: extrato aquoso e etanólico de casca de pequi (IC50 igual a 9,44 e 17,98 µg.mL-1 respectivamente, extrato etanólico de sementes de cagaita (IC50 igual a 14,15 µg.mL-1, extrato etanólico de sementes e casca de araticum (IC50 igual a 30,97 e 49,18 µg.mL-1, respectivamente. Este é o primeiro estudo que avalia o potencial em seqüestrar radicais livres de frações de frutas do cerrado. Os resultados indicam que os extratos possuem grande potencial antioxidante e estudos adicionais são necessários para avaliar essa propriedade dos extratos como uma aplicação sustentável dos recursos do cerrado nos setores farmacêuticos, cosméticos e nutricionais.Annona crassiflora (araticum, Solanum lycocarpum (lobeira, Eugenia dysenterica (cagaita, Caryocar brasilense (pequi and Swartzia langsdorfii (banha de galinha are tropical fruits consumed mainly by native people in the Brazilian Cerrado (second biggest biome of Brazil. In this study, pulp, seed and peel of the fruits were extracted using ethanol and water. Some of the extracts showed a high content of total phenols and were screened for their potential as antioxidants using the in vitro model 2,2-diphenyl-1-picryl hydrazyl (DPPH. The best results were found for aqueous and ethanolic extracts of pequi peel (IC50 of 9,44 and 17,98 µg.mL-1 respectively

Effect Of Various Substrates On Eudrilus eugeniae (Oligochaeta Production

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Mpoame, M.

1994-01-01

Full Text Available In order to assess the effects of substrate on Eudrilus eugeniae production, 360 worms were raised in plastic buckets containing manure either from cattle (BV, from sheep (CM, from rabbit (CL, and from chicken (FP. At the end of the experiment which lasted 4 months, worm productivity was estimated at 111.9 g/kg of substrate in CL, 86.3 g/kg in CM, and 33.0 g/kg in BV. Substrate FP was inadequate as worms escaped from it. In another trial aiming at determining some of E. Eugeniae's reproductive parameters, each of the 3 substrates BV, CL, and CM was distributed into 15 plastic half bottles and was inoculated with one cocoon. In the 3 substrates cocoon incubation time and growing period were estimated at 3 to 4 weeks and 6 to 7 weeks respectively. Cocoons were laid approximately a week after the pairing of worms. On the average, each worm laid 2 cocoons per week.

Evaluation of antioxidant, immunomodulatory, and cytotoxic action of fractions from Eugenia uniflora L. and Eugenia malaccensis L.: correlation with polyphenol and flavanoid content.

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Figueirôa, Evellyne de Oliveira; Nascimento da Silva, Luís Cláudio; de Melo, Cristiane Moutinho Lagos; Neves, Juliana Kelle de Andrade Lemoine; da Silva, Nicácio Henrique; Pereira, Valéria Rêgo Alves; Correia, Maria Tereza dos Santos

2013-01-01

An increasing number of biological activities presented by medicinal plants has been investigated over the years, and they are used in the search for new substances with lower side effects. Eugenia uniflora L. and Eugenia malaccensis L. (Myrtaceae) have many folk uses in various countries. This current study was designed to quantify the polyphenols and flavonoids contents and evaluate the immunomodulatory, antioxidant, and cytotoxic potentials of fractions from E. uniflora L. and E. malaccensis L. It was observed that the polyphenol content was higher in ethyl acetate fractions. These fractions have high antioxidant potential. E. malaccensis L. seeds showed the largest DPPH radical scavenger capacity (EC50 = 22.62). The fractions of E. malaccensis L. leaves showed lower antioxidant capacity. The samples did not alter the profile of proinflammatory cytokines and nitric oxide release. The results indicate that species of the family Myrtaceae are rich in compounds with antioxidant capacity, which can help reduce the inflammatory response.

Arquivo de Antropologia Física do Museu Nacional: fontes para a história da eugenia no Brasil The National Museum's physical anthropology archive: sources on the history of eugenics in Brazil

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Vanderlei Sebastião de Souza

2009-09-01

Full Text Available Apresenta um conjunto de fontes documentais que integram o Arquivo de Antropologia Física, de responsabilidade do Setor de Antropologia Biológica do Museu Nacional/UFRJ. O arquivo contém importante documentação sobre o Primeiro Congresso Brasileiro de Eugenia, realizado em 1929, no Rio de Janeiro, em comemoração ao centenário da Academia Nacional de Medicina. Além de originais dos trabalhos apresentados no evento - alguns inéditos -, constam também nesse arquivo documentos reunidos pela secretaria do Congresso, como convocações para a sua realização, ficha dos inscritos, correspondências, recortes de jornais e revistas com artigos sobre eugenia, bem como moções, relatórios e atas finais, constituindo acervo fundamental para a compreensão da história da eugenia no Brasil.The article presents a set of documental sources that are part of the physical anthropology archive administered by the National Museum's biological anthropology sector (UFRJ. The archive holds important documentation on the first Brazilian congress of eugenics, held in Rio de Janeiro in 1929, in celebration of the National Academy of Medicine centennial. In addition to the originals of papers presented at the event (some unpublished, the archive also contains a series of documents compiled by the congress organizers, including announcements of the event, attendee registrations, correspondence, newspaper and magazine clippings of articles on eugenics, as well as motions, reports, and final minutes, all of which makes this collection invaluable in understanding the history of eugenics in Brazil.

Various compositions containing organic substrates to produce Eugenia uniflora L. seedlings

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Licielo Romero Vieira

2015-09-01

Full Text Available The use of substrates formulated with agro-industrial residues, such as coconut shell fiber, sawdust, and carbonized rice husk, is among the sustainable alternatives to decrease the production costs of seedlings of plant species. This study aimed to evaluate the use of various substrates for obtaining high quality Eugenia uniflora L. seedlings. The experiment was conducted at the Federal University of Pampa (UNIPAMPA – Campus in São Gabriel, Rio Grande do Sul, Brazil, by sowing directly into 200 cm3 polypropylene tubes, testing various substrates (50% commercial substrate Plantmax® + 50% rice husk; commercial substrate Plantmax®; 50% commercial substrate Plantmax® + 50% coconut shell fiber; and 50% commercial substrate Plantmax® + 50% sawdust. Every 3 days, the number of emerged seedlings was evaluated and, after 180 days, the following morphological features were analyzed: shoot height, stem diameter, number of leaves, root and total fresh weight, shoot, root, and total dry weight, and Dickson’s quality index. The results indicate that all substrates were suitable for Eugenia uniflora emergence, but that containing 50% commercial substrate Plantmax® + 50% sawdust provided a delay in the emergence of this Myrtaceae; also, substrates containing 50% commercial substrate Plantmax® + 50% husk rice and 50% commercial substrate Plantmax® + 50% coconut shell fiber showed to be satisfactory for the growth of Eugenia uniflora seedlings.

Chemical composition of essential oils of Eugenia caryophylla and ...

African Journals Online (AJOL)

Results: The essential oils obtained yielded of 5.9 for Eugenia caryophylla and 0.2% Mentha sp cf piperita respectively. The chemical composition was assigned by GC and GC/SM and showed that E. caryophylla was mainly composed of eugenol (80.0 %), E-caryophyllene (8.3%), and eugenol acetate (6.7%) while Mentha ...

Activity of in vitro forms of dentifrices containing the hydroalcoholic extract of the ripe fruit of Eugenia uniflora L. (Surinam cherry) on cariogenic bacteria.

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Jovito, Vanessa C; Freires, Irlan A; Almeida, Leopoldina F D; Moura, Douglas; Castro, Ricardo D; Paulo, Marçal Q; Leite-Cavalcanti, Alessandro

2011-01-01

The aim of this study was to evaluate the in vitro activity of dentifrices containing the hydroalcoholic extract of the ripe fruit of Eugenia uniflora L. (Surinam cherry) on Streptococcus oralis (ATCC 10557) and Lactobacillus casei (ATCC 7469). Five dentifrices were used: D1: containing hydroalcoholic extract of Eugenia uniflora L.; D2: containing fluoride and hydroalcoholic extract of Eugenia uniflora L.; D3: containing triclosan and hydroalcoholic extract of Eugenia uniflora L; D4: containing triclosan, fluoride and hydroalcoholic extract of Eugenia uniflora L.; D5: positive control (Colgate Total 12). To determine the antibacterial activity, the technique used was the minimum inhibitory concentration by the diffusion method in solid culture medium. At the concentration 0.05 g/mL, the best results were achieved with D1 (18 mm) and D4 (24 mm) on L.casei, and with D3 (19 mm) on S. oralis. The dentifrices D3 and D4 were found to have greater activity on the Streptococcus oralis, while D4 and D1 were found to have greater activity on Lactobaccilus casei. It is concluded that dentifrices with Eugenia uniflora L. have antimicrobial activity, suggesting that clinical trials should be conducted.

O gênero Eugenia L. (Myrtaceae na planície de alagável do Alto Rio Paraná, Estados de Mato Grosso do Sul e Paraná, Brasil The genus Eugenia L. (Myrtaceae on the Upper Paraná River floodplain, Mato Grosso do Sul and Paraná States, Brazil

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Mariza Barion Romagnolo

2006-09-01

Full Text Available Foi realizado o levantamento das espécies de Eugenia L. da planície alagável do Alto Rio Paraná, Estados de Mato Grosso do Sul e Paraná (22º40' a 22º55'S e 53º10' a 53º40'W. Esta área localiza-se no domínio da Floresta Estacional Semidecidual e apresenta formações ripárias ao longo do rio Paraná, de suas ilhas e de seus tributários. As espécies registradas foram: Eugenia egensis DC., E. florida DC., E. hyemalis Cambess., E. klappenbachiana Mattos & D. Legrand, E. moraviana O. Berg, E. pyriformis Cambess., E. ramboi D. Legrand, E. repanda O. Berg., E. sulcata Spring. ex Mart. e E. uniflora L. Os meses que apresentaram o maior número de espécies em floração e frutificação foram respectivamente, setembro e novembro. Eugenia florida, E. hyemalis e E. repanda apresentam ampla distribuição na área, enquanto que E. ramboi e E. sulcata foram encontradas somente na margem esquerda do rio Paraná. É fornecida uma chave para a identificação das espécies, acompanhada de ilustrações e descrições das mesmas, além de informações sobre o período de floração, frutificação e distribuição na área estudada.A floristic survey of Eugenia L. species of the Upper Paraná River floodplain in Mato Grosso do Sul and Paraná States, Brazil (22º40'-22º55'S; 53º10'-53º40'W is presented. The study area is covered by Seasonal Semideciduous Forest, with riparian formations along the Paraná River and its islands and tributaries. The following species were identified: E. egensis DC., E. florida DC., E. hyemalis Cambess., E. klappenbachiana Mattos & D. Legrand, E. moraviana O. Berg, E. pyriformis Cambess., E. ramboi D. Legrand, E. repanda O.Berg., E. sulcata Spring. ex Mart., and E. uniflora L. The greatest number of species in flower or fruit was detected in September and November, respectively. Eugenia florida, E. hyemalis and E. repanda are widely distributed in the study area, whereas E. ramboi and E. sulcata were observed only

Chemical composition and evaluation of the antimicrobial activity of the essential oil from leaves of Eugenia platysema.

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Tenfen, Adrielli; Siebert, Diogo Alexandre; Yamanaka, Celina Noriko; Mendes de Córdova, Caio Maurício; Scharf, Dilamara Riva; Simionatto, Edésio Luiz; Alberton, Michele Debiasi

2016-09-01

This study describes the qualitative and quantitative chemical composition and evaluates the antibacterial activity of essential oil from Eugenia platysema leaves. Analysis by GC-FID and GC-MS allowed the identification of 22 compounds. Different from the other species of the Eugenia genus, the major compound found in the essential oil was the diterpene phytol (66.05%), being this the first report of the presence of this compound in the essential oils from Eugenia genus. The sesquiterpene elixene was the second most concentrated compound in the studied essential oil (9.16%). The essential oil from E. platysema was tested for its antibacterial activity against cell-walled bacteria and mollicute strains of clinical interest using the microdilution broth assay. The results showed that the essential oil of E. platysema was inactive until 1000 μg mL(-1) against tested bacteria.

Antioxidant activity and phenolic content of leaf infusions of Myrtaceae species from Cerrado (Brazilian Savanna

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L. K. Takao

Full Text Available Abstract There is considerable interest in identifying new antioxidants from plant materials. Several studies have emphasized the antioxidant activity of species belonging to the Myrtaceae family. However, there are few reports on these species from the Cerrado (Brazilian savanna. In this study, the antioxidant activity and phenolic content of 12 native Myrtaceae species from the Cerrado were evaluated (Blepharocalyx salicifolius, Eugenia bimarginata, Eugenia dysenterica, Eugenia klotzschiana, Hexachlamys edulis, Myrcia bella, Myrcia lingua, Myrcia splendens, Myrcia tomentosa, Psidium australe, Psidium cinereum, and Psidium laruotteanum. Antioxidant potential was assessed using the antioxidant activity index (AAI by the DPPH method and total phenolic content (TPC by the Folin–Ciocalteu assay. There was a high correlation between TPC and AAI values. Psidium laruotteanum showed the highest TPC (576.56 mg GAE/g extract and was the most potent antioxidant (AAI = 7.97, IC50 = 3.86 µg·mL−1, with activity close to that of pure quercetin (IC50 = 2.99 µg·mL−1. The extracts of nine species showed IC50 of 6.24–8.75 µg·mL−1. Most species showed TPC and AAI values similar to or higher than those for Camellia sinensis, a commonly consumed tea with strong antioxidant properties. The results reveal that the analyzed Myrtaceae species from the Cerrado possess high phenolic contents and antioxidant activities. Thus, they are a potential source of new natural antioxidants.

Biodegradation of Garden Waste, Market Waste Using Eisenia fetida and Eudrilus eugenia and Assessment of Manure Quality on Tomato

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Mohan, S. Mariraj

2014-06-01

Comparative study was performed to evaluate the vermicomposting efficiency of two earthworm species Eisenia fetida, Eudrilus eugenia from the garden wastes, vegetable market wastes. Three different experimental works were conducted. For each experiment three plastic vermibins were used. Experiment (1) mentioned for control without earthworms. Experiment (2) bedded with Eudrilus eugenia, Experiment (3) comprised of bedding with Eisenia fetida. Pre composting was allowed for 10 days after that Eudrilus eugenia, Eisenia fetida were added in respective vermibins. The multiplication of earthworms in terms of number was calculated at the end of vermicomposting. The N, P, K value of the manure in each vermibin was estimated before and after the completion of the experiment. High N, P, K value was obtained in Experiment (2) and Experiment (3) compared to control. Among the solid wastes, the vegetable wastes were degraded quickly by Eudrilus eugenia and also it has the best quality of manure. Eudrilus eugenia was found to be efficient for quick degradation of both garden wastes and vegetable wastes. After manure production, field trials were conducted using different fertilizers to assess the manure quality in the growth and yield of tomato plants. Six types of experimental trial pots were prepared where one was kept as control and five others were treated with different category of fertilizers. The treatment pots (P3) showed better growth parameters (leaf numbers, stem diameter, plant height) than the rest of the trial.

α-Glucosidase inhibitory hydrolyzable tannins from Eugenia jambolana seeds.

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Omar, Raed; Li, Liya; Yuan, Tao; Seeram, Navindra P

2012-08-24

Three new hydrolyzable tannins including two gallotannins, jamutannins A (1) and B (2), and an ellagitannin, iso-oenothein C (3), along with eight known phenolic compounds were isolated from the seeds of Eugenia jambolana fruit. The structures were elucidated on the basis of spectroscopic data analysis. All compounds isolated were evaluated for α-glucosidase inhibitory effects compared to the clinical drug acarbose.

The effect of fresh leaf Ocimum gratissimum and dried buds Eugenia ...

African Journals Online (AJOL)

Bulletin of Animal Health and Production in Africa ... Abstract. The use of antibiotics has lead to antibiotic resistance and residual effects among others in fish tissue thus necessitate the advocate for alternative natural plants for ... Keywords: Antimicrobial, aquaculture, Eugenia caryophyllata, extracts, Ocimum gratissimum ...

Bioremediation of heavy metals and petroleum hydrocarbons in diesel contaminated soil with the earthworm: Eudrilus eugeniae.

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Ekperusi, Ogheneruemu Abraham; Aigbodion, Iruobe Felix

2015-01-01

A laboratory study on the bioremediation of diesel contaminated soil with the earthworm Eudrilus eugeniae (Kingberg) was conducted. 5 ml of diesel was contaminated into soils in replicates and inoculated with E. eugeniae for 90 days. Physicochemical parameters, heavy metals and total petroleum hydrocarbons were analyzed using AAS. BTEX in contaminated soil and tissues of earthworms were determined with GC-FID. The activities of earthworms resulted in a decrease in pH (3.0 %), electrical conductivity (60.66 %), total nitrogen (47.37 %), chloride (60.66 %), total organic carbon (49.22 %), sulphate (60.59 %), nitrate (60.65 %), phosphate (60.80 %), sodium (60.65 %), potassium (60.67 %), calcium (60.67 %), magnesium (60.68 %), zinc (60.59 %), manganese (60.72 %), copper (60.68 %), nickel (60.58 %), cadmium (60.44 %), vanadium (61.19 %), chromium (53.60 %), lead (60.38 %), mercury (61.11 %), arsenic (80.85 %), TPH (84.99 %). Among the BTEX constituents, only benzene (8.35 %) was detected in soil at the end of the study. Earthworm tissue analysis showed varying levels of TPH (57.35 %), benzene (38.91 %), toluene (27.76 %), ethylbenzene (42.16 %) and xylene (09.62 %) in E. eugeniae at the end of the study. The study has shown that E. eugeniae could be applied as a possible bioremediator in diesel polluted soil.

Diferentes composições com substratos orgânicos na produção de mudas de Eugenia uniflora L.

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Licielo Romero Vieira

2015-05-01

Full Text Available http://dx.doi.org/10.5007/2175-7925.2015v28n3p43 A utilização de substratos formulados com resíduos da agroindústria, tais como a fibra de coco, a serragem e a casca de arroz carbonizada, é uma das alternativas sustentáveis visando a diminuir os custos de produção de mudas de espécies vegetais. Este estudo teve por objetivo avaliar a utilização de diferentes substratos na obtenção de mudas de Eugenia uniflora L. de alta qualidade. O experimento foi conduzido na Universidade Federal do Pampa (Unipampa – Campus São Gabriel-RS, com semeadura diretamente em tubetes de polipropileno de 200 cm3, testando diferentes substratos (50% de substrato comercial Plantmax® + 50% de casca de arroz; substrato comercial Plantmax®; 50% de substrato comercial Plantmax® + 50% de fibra de casca de coco; e 50% de substrato comercial Plantmax® + 50% de serragem. A cada três dias foram avaliados o número de plântulas emergidas e, após 180 dias, as seguintes características morfológicas foram analisadas: altura da parte aérea, diâmetro do coleto, número de folhas, massa fresca radicular e total, massa seca da parte aérea, radicular e total e o índice de qualidade de Dickson. Os resultados indicam que todos os substratos foram adequados à emergência de Eugenia uniflora, mas aquele com 50% de substrato comercial Plantmax® + 50% de serragem proporcionou um atraso na emergência dessa Myrtaceae; além disso, os substratos com 50% de substrato comercial Plantmax® + 50% casca de arroz e 50% substrato comercial Plantmax® + 50% fibra de casca de coco mostraram-se satisfatórios para o crescimento de mudas de Eugenia uniflora.

Eugenia 'negativa', psiquiatria e catolicismo: embates em torno da esterilização eugênica no Brasil 'Negative' eugenics, psychiatry, and Catholicism: clashes over eugenic sterilization in Brazil

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Robert Wegner

2013-03-01

Full Text Available Analisa o diálogo do eugenista Renato Kehl com um grupo de psiquiatras brasileiros que, no início da década de 1930, aproximaram-se da chamada eugenia negativa. Entusiasmados com as pesquisas e a aplicação de medidas eugênicas em países como os EUA e a Alemanha, autores como Ernani Lopes, Ignácio da Cunha Lopes, Alberto Farani e Antonio Carlos Pacheco e Silva elegeram a religião católica como empecilho para que o Brasil pudesse seguir caminho semelhante, especialmente quanto à resistência à implantação da esterilização dos ditos 'degenerados' que passara a vigorar na Alemanha em 1934. O artigo mapeia as diferentes estratégias propostas pelos autores para dialogar com a Igreja católica.The article analyzes the dialogue between eugenicist Renato Kehl and a group of Brazilian psychiatrists who turned their interest to so-called negative eugenics in the early 1930s. Enthused about research into eugenics and the application of eugenic methods in countries such as the United States and Germany, authors like Ernani Lopes, Ignácio da Cunha Lopes, Alberto Farani, and Antonio Carlos Pacheco e Silva blamed Catholicism for impeding Brazil from moving in a similar direction, especially the church's resistance to the sterilization of 'degenerates', which entered into effect in Germany in 1934. The article charts the various strategies these authors proposed for engaging in dialogue with the Catholic Church.

New gall midges (Diptera, Cecidomyiidae) associated with Eugenia uniflora and Psidium cattleianum (Myrtaceae)

OpenAIRE

Maia,Valéria C; Nava,Dori E

2011-01-01

Two new species and a new genus of gall midges (Diptera, Cecidomyiidae) are described and illustrated. Both species induce leaf galls on Myrtaceae, the former on Eugenia uniflora and the latter on Psidium cattleianum.

The evolutionary history of Eugenia sect. Phyllocalyx (Myrtaceae) corroborates historically stable areas in the southern Atlantic forests.

Science.gov (United States)

de Oliveira Bünger, Mariana; Fernanda Mazine, Fiorella; Forest, Félix; Leandro Bueno, Marcelo; Renato Stehmann, João; Lucas, Eve J

2016-12-01

Eugenia sect. Phyllocalyx Nied. includes 14 species endemic to the Neotropics, mostly distributed in the Atlantic coastal forests of Brazil. Here the first comprehensive phylogenetic study of this group is presented, and this phylogeny is used as the basis to evaluate the recent infrageneric classification in Eugenia sensu lato (s.l.) to test the history of the evolution of traits in the group and test hypotheses associated with the history of this clade. A total of 42 taxa were sampled, of which 14 were Eugenia sect. Phyllocalyx for one nuclear (ribosomal internal transcribed spacer) and four plastid markers (psbA-trnH, rpl16, trnL-rpl32 and trnQ-rps16). The relationships were reconstructed based on Bayesian analysis and maximum likelihood. Additionally, ancestral area analysis and modelling methods were used to estimate species dispersal, comparing historically climatic stable (refuges) and unstable areas. Maximum likelihood and Bayesian inferences indicate that Eugenia sect. Phyllocalyx is paraphyletic and the two clades recovered are characterized by combinations of morphological characters. Phylogenetic relationships support a link between Cerrado and south-eastern species and a difference in the composition of species from north-eastern and south-eastern Atlantic forest. Refugia and stable areas identified within unstable areas suggest that these areas were important to maintain diversity in the Atlantic forest biodiversity hotspot. This study provides a robust phylogenetic framework to address important historical questions for Eugenia s.l. within an evolutionary context, supporting the need for better taxonomic study of one of the largest genera in the Neotropics. Furthermore, valuable insight is offered into diversification and biome shifts of plant species in the highly environmentally impacted Atlantic forest of South America. Evidence is presented that climate stability in the south-eastern Atlantic forest during the Quaternary contributed to the

Caracterização genética de uma coleção de germoplasma de cagaiteira, uma espécie nativa do cerrado

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Edivaldo Barbosa de Almeida Júnior

2014-09-01

Full Text Available A cagaiteira é uma espécie frutífera comum no bioma Cerrado. A planta produz um fruto do tipo baga que é consumido in natura ou processado de várias formas. Em função do potencial produtivo da espécie, existe interesse na sua domesticação, para produção em larga escala. Para atender esse objetivo, a avaliação da variabilidade genética é fundamental para subsidiar programas de melhoramento e domesticação da espécie. Nesse sentido, o objetivo do trabalho foi estimar parâmetros genéticos quantitativos da coleção de germoplasma de Eugenia dysenterica DC., para disponibilizar informações que possam ser úteis em programas de melhoramento da espécie. O experimento foi montado no ano de 1998 em delineamento experimental de blocos completos ao acaso com 110 tratamentos e quatro repetições. Foram avaliadas as variáveis das plantas que incluem a altura (AP, altura da primeira bifurcação (AB, circunferência do caule a 10 cm do solo (CC, projeção média da copa (DC e variáveis das folhas, que incluem o comprimento do limbo (CL, largura do limbo (LL, formato das folhas (FF e comprimento do pecíolo (CP. Os coeficientes de herdabilidade ao nível das médias variaram entre 87,66% e 25,16% e os coeficientes de variação genética, entre 45% e 7%, valores próximos aos obtidos para outras espécies silvestres do Cerrado. As estimativas dos parâmetros sugerem respostas modestas à seleção para as variáveis das plantas e respostas expressivas para as variáveis das folhas.

Eugenia uniflora L. Essential Oil as a Potential Anti-Leishmania Agent: Effects on Leishmania amazonensis and Possible Mechanisms of Action

OpenAIRE

Rodrigues, Klinger Antonio da Franca; Amorim, Layane Val?ria; de Oliveira, Jamylla Mirck Guerra; Dias, Clarice Noleto; Moraes, Denise Fernandes Coutinho; Andrade, Eloisa Helena de Aguiar; Maia, Jose Guilherme Soares; Carneiro, Sabrina Maria Portela; Carvalho, Fernando A?cio de Amorim

2013-01-01

Eugenia uniflora L. is a member of the Myrtaceae family and is commonly known as Brazilian cherry tree. In this study, we evaluated the chemical composition of Eugenia uniflora L. essential oil (EuEO) by using gas chromatography-mass spectrometry (GC-MS) and assessed its anti-Leishmania activity. We also explored the potential mechanisms of action and cytotoxicity of EuEO. Thirty-two compounds were identified, which constituted 92.65% of the total oil composition. The most abundant components...

Chemical Composition of Four Essential Oils of Eugenia from the Brazilian Amazon and Their Cytotoxic and Antioxidant Activity.

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da Silva, Joyce Kelly R; Andrade, Eloisa Helena A; Barreto, Leilane H; da Silva, Nádia Carolina F; Ribeiro, Alcy F; Montenegro, Raquel C; Maia, José Guilherme S

2017-07-08

Background: Eugenia species are appreciated for their edible fruits and are known as having anticonvulsant, antimicrobial and insecticidal actions. Methods: The plant material was collected in the southeastern Pará state of Brazil and submitted to hydrodistillation. GC-MS analyzed the oils, and their antioxidant and cytotoxic activities were evaluated by the DPPH and MTT assays. Results: The main components identified in the Eugenia oils were 5-hydroxy- cis -calemene, (2 E ,6 E )-farnesol, (2 E ,6 Z )-farnesol, caryophylla-4(12),8(13)-dien-5α-ol-5β-ol, E -γ-bisabolene, β-bisabolene, germacrene D, and ishwarane. The oil of E. egensis showed the most significant antioxidant activity (216.5 ± 11.6 mg TE/mL), followed by the oils of E. flavescens (122.6 ± 6.8 mg TE/mL) and E. patrisii (111.2 ± 12.4 mg TE/mL). Eugenia oils were cytotoxic to HCT-116 (colon cancer) cells by the MTT assay, where the most active was the oil of E. polystachya (10.3 µg/mL), followed by the oils of E. flavescens (13.9 µg/mL) and E. patrisii (16.4 µg/mL). The oils of E. flavescens and E. patrisii showed the highest toxicity for MRC5 (human fibroblast) cells, with values of 14.0 µg/mL and 18.1 µg/mL, respectively. Conclusions: These results suggest that Eugenia oils could be tested in future studies for the treatment of colon cancer and oxidative stress management.

Studies of Malagasy Eugenia – IV: Seventeen new endemic species, a new combination, and three lectotypifications; with comments on distribution, ecological and evolutionary patterns

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Neil Snow

2015-04-01

Full Text Available Seventeen new endemic species of the genus Eugenia L. (Myrtaceae are proposed from Madagascar, including: E. andapae N. Snow, E. barriei N. Snow, E. bemangidiensis N. Snow, E. calciscopulorum N. Snow, E. delicatissima N. Snow, Callm. & Phillipson, E. echinulata N. Snow, E. gandhii N. Snow, E. hazonjia N. Snow, E. iantarensis N. Snow, E. malcomberi N. Snow, E. manomboensis N. Snow, E. obovatifolia N. Snow, E. ranomafana N. Snow & D. Turk, E. ravelonarivoi N. Snow & Callm., E. razakamalalae N. Snow & Callm., E. tiampoka N. Snow & Callm., and E. wilsoniana N. Snow, and one new combination, Eugenia richardii (Blume N. Snow, Callm. & Phillipson is provided. Detailed descriptions, information on distribution and ecology, distribution maps, vernacular names (where known, digital images of types, comparisons to morphologically similar species. Preliminary assessment of IUCN risk of extinction and conservation recommendations are provided, including Vulnerable (4 species, Endangered (2 species, and Critically Endangered (4 species. Lectotpyes are designated for Eugenia hovarum H. Perrier, Eugenia nompa H. Perrier, and E. scottii H. Perrier respectively.

Mapping stable direct and retrograde orbits around the triple system of asteroids (45) Eugenia

Science.gov (United States)

Araujo, R. A. N.; Moraes, R. V.; Prado, A. F. B. A.; Winter, O. C.

2017-12-01

It is widely accepted that knowing the composition and the orbital evolution of asteroids might help us to understand the process of formation of the Solar system. It is also known that asteroids can represent a threat to our planet. Such an important role has made space missions to asteroids a very popular topic in current astrodynamics and astronomy studies. Taking into account the increasing interest in space missions to asteroids, especially to multiple systems, we present a study that aims to characterize the stable and unstable regions around the triple system of asteroids (45) Eugenia. The goal is to characterize the unstable and stable regions of this system and to make a comparison with the system 2001 SN263, which is the target of the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) mission. A new concept was used for mapping orbits, by considering the disturbance received by the spacecraft from all perturbing forces individually. This method has also been applied to (45) Eugenia. We present the stable and unstable regions for particles with relative inclination between 0° and 180°. We found that (45) Eugenia presents larger stable regions for both prograde and retrograde cases. This is mainly because the satellites of this system are small when compared to the primary body, and because they are not close to each other. We also present a comparison between these two triple systems, and we discuss how these results can guide us in the planning of future missions.

Evaluation of some pharmacological activities of Eugenia uniflora L.

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Schapoval, E E; Silveira, S M; Miranda, M L; Alice, C B; Henriques, A T

1994-12-01

In view of the extensive use of Eugenia uniflora in folk medicine, different extracts of dried and fresh leaves of the plant were assayed to test its possible pharmacological activities. The infusion of fresh leaves had a highly significant anti-inflammatory effect when administered p.o. to rats 1 h before subplantar injection of carrageenin. The infusion increased the pentobarbital sleeping time and also had an effect on intestinal transit, and had no acute toxic effect. No analgesic or antimicrobial activities were observed with any of the extracts used.

Seasonal variation in the phenol content of Eugenia uniflora L. leaves

OpenAIRE

Santos, R.M; Oliveira, M.S; Ferri, P.H; Santos, S.C

2011-01-01

Hydrolysable tannins, total phenols and flavonoids in Eugenia uniflora leaves were monthly analysed for one year. The results were correlated with climate conditions (rainfall, humidity, cloudiness and mean temperature) through chemometric methods. Principal component analysis revealed high levels of hydrolysable tannins in the rainy season, whereas flavonoids were mainly produced in the dry season. These facts suggest that climatic changes may be one of the factors affecting phenol levels in...

Potencial nutritivo de frutos de pitangão (Eugenia neonitida, Sobral Nutritive potential of pitangão (Eugenia neonitida, Sobral fruits and seeds

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Juliana dos Santos Vilar

2006-12-01

Full Text Available Eugenia neonitida, Sobral (Myrtaceae ocorre naturalmente nas restingas dos Estados do Rio de Janeiro e Espírito Santo, possui hábito arbustivo e pode alcançar até 2,5m de altura. Floresce entre os meses de outubro e novembro, frutificando de novembro a janeiro. Seus frutos, popularmente chamados de "Pitangão", são oblongos, e durante o processo de amadurecimento sua cor evolui do verde até o amarelo vivo quando maduro. Apresentam sabor agridoce e exalam fragrância agradável, tornando-os amplamente apreciados pela população local. Determinações analíticas revelaram que 100g de polpa deste fruto contêm alto teor de umidade (93,2% e baixo valor calórico, 54,21 kcal, oriundo de 3,21 ± 0,25g de lipídeos, 2,2 ± 0,86g de proteínas, 0,55 ± 0,03 g de carboidratos, além de 6g de sólidos solúveis e alta concentração de minerais, com destaque para o sódio, 480,8mg.100g-1 de polpa do fruto. A acidez total é de 1,38 ± 0,03g de NaOH.100g-1, o pH é 2,85 e a concentração de ácido ascórbico é de 17,86 ± 0,06mg.100g-1. O valor de beta-caroteno é de 60 ± 0,04 mg.100g-1. Comparando-se a polpa do pitangão com a da pitanga (Eugenia uniflora L., foram constatados maiores valores nutricionais para a primeira. O pitangão pode ser uma ótima fonte de macro e micronutrientes para alimentação humana, embora ainda não seja cultivada nem comercializada.Eugenia neonitida, Sobral (Myrtaceae occurs naturally in the states of Rio de Janeiro and Espírito Santo. Generally, it is a shrub, but it can reach up to 2,5m of height. It blooms from August to November and fructifies from October to December. Their fruits, popularly called "Pitangão", are oblong and during the maturation process the fruit color turns from green to bright yellow when ripe. They present a bittersweet taste and a pleasant fragrance, what makes much appreciated by local population. Analytical determinations revealed that 100g of pulp possess high concentration of

Rediscovery of Eugenia fajardensis (Myrtaceae), a rare tree from the Puerto Rican Bank

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Jorge C. Trejo-Torres; Marcos A. Caraballo-Ortiz; Miguel A. Vives-Heyliger; Christian W. Torres-Santana; William Cetzal-Ix; Joel A. Mercado-Diaz; Tomas A. Carlo

2014-01-01

Eugenia fragrans var.? fajardensis was described in 1895 and raised to species status in 1923 as E. fajardensis. In 1925, it was relegated to the synonymy of Anamomis fragrans (Myrcianthes fragrans). Since 2001, we have re-discovered wild plants and herbarium specimens, including a previously unidentified isotype of E. fajardensis, supporting the validity of this...

Regeneração natural de cerrado sob plantio de Eucalyptus camaldulensis Dehn. no norte de Minas Gerais, Brasil

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Michellia Pereira Soares

2013-04-01

Full Text Available A formação de paisagens homogêneas tornou-se um problema na biologia da conservação, já que essas plantações interferem diretamente na dinâmica das comunidades. Sendo assim, o objetivo deste trabalho foi determinar a composição florística e a estrutura da regeneração natural de cerrado, no sub-bosque de um plantio de Eucalyptus camaldulensis, e verificar se essa regeneração se diferencia, dependendo da distância em relação às bordas do talhão. O estudo foi realizado no município de Montes Claros, MG. Para o levantamento, foram demarcados dois blocos, cada um subdividido em nove parcelas de 10 m x 10 m, utilizando-se, como critério de inclusão, a altura > 0,30 m. Foram registrados 2.413 indivíduos. No bloco 1, foram amostrados 914 indivíduos, pertencentes a 63 espécies e 26 famílias, sendo Eugenia dysenterica, Hymenaea stigonogocarpa, Calliandra sp. e Duguetia furfuracea as espécies mais importantes, segundo o Valor de Importância (VI. No bloco 2, foram registrados 1.499 indivíduos, distribuídos em 71 espécies e 30 famílias, sendo os maiores VIs alcançados por Cupania vernalis, Chamaecrista desvauxii, Pouteria ramiflora . Senna rugosa. A distribuição em classes de altura e de diâmetro mostrou a maioria dos indivíduos nas menores classes, demonstrando que a área ainda reflete um evento de regeneração recente. Entretanto, os resultados deste estudo indicam que existem comunidades distintas no local, estando o bloco 2 em estádio mais avançado de regeneração do que o primeiro. Esse fato foi confirmado pela análise de agrupamento entre as parcelas e as diferenças de diversidade e desenvolvimento estrutural da vegetação de cada bloco.

Seasonal variation in the phenol content of Eugenia uniflora L. leaves Variação sazonal nos teores de fenóis de folhas de Eugenia uniflora L

OpenAIRE

R.M Santos; M.S Oliveira; P.H Ferri; S.C Santos

2011-01-01

Hydrolysable tannins, total phenols and flavonoids in Eugenia uniflora leaves were monthly analysed for one year. The results were correlated with climate conditions (rainfall, humidity, cloudiness and mean temperature) through chemometric methods. Principal component analysis revealed high levels of hydrolysable tannins in the rainy season, whereas flavonoids were mainly produced in the dry season. These facts suggest that climatic changes may be one of the factors affecting phenol levels in...

EFEITO DE FUNGOS MICORRÍZICOS ARBUSCULARES E DA ADUBAÇÃO NO CRESCIMENTO DE MUDAS DE Eugenia uniflora L., PRODUZIDAS EM DIFERENTES SUBSTRATOS

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SAMANTA JAQUELINE DALANHOL

2016-02-01

Full Text Available RESUMO O objetivo deste estudo foi avaliar o efeito de fungos micorrízicos arbusculares (FMAs, da adubação e da composição do substrato no crescimento de mudas de Eugenia uniflora. As sementes foram germinadas em vermiculita média e repicadas para tubetes (100 cm3 contendo substratos à base de vermicomposto e casca de arroz carbonizada e, como controle, utilizou-se do substrato comercial à base de casca de pínus. Estes substratos foram testados com e sem inoculação micorrízica, adicionada ao substrato, como também se testaram a presença e a ausência de adubação de cobertura. Foram analisadas as propriedades físico-químicas dos substratos formulados. Avaliaram-se a altura, o diâmetro do colo, a agregação das raízes ao substrato, a biomassa seca aérea, a biomassa seca radicial e foram determinados a relação entre altura e diâmetro do colo e o índice de qualidade de Dickson. A inoculação com FMAs não influenciou no crescimento das mudas, enquanto a interação entre substratos e adubação foi significativa para a maioria das variáveis. A ausência de resposta aos FMAs foi, provavelmente, devido às altas concentrações de fósforo nestes substratos. Concluiu-se que o substrato à base de vermicomposto e casca de arroz carbonizada, na proporção de 20/80, pode ser utilizado na produção de mudas desta espécie.

Eugenesia: Un análisis histórico y una posible propuesta Eugenia: Uma análise histórica e uma possível proposta Eugenics: A historical analysis and a possible proposal

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Fabiola Villela Cortés

2011-11-01

Full Text Available La eugenesia es un tema abordado, entre otros, por historiadores, filósofos, médicos, bioeticistas, por distintas razones. La idea de mejorar la raza humana siempre ha estado presente en la historia de la civilización, también han sido objeto de comentarios el intento de consolidarla como una ciencia, la presencia de movimientos eugenésicos en varios países del mundo, el holocausto nazi y, finalmente, el resurgimiento de la eugenesia a raíz de la decodificación del genoma humano. Nuestro objetivo es dar un repaso por los movimientos eugenésicos que tuvieron lugar a mediados del siglo XX, el resurgimiento de la eugenesia y los adelantos con los que contamos actualmente.A eugenia é um tema abordado, entre outros, por historiadores, filósofos, médicos, bioeticistas, e por diferentes razões. A ideia de melhorar a raça humana sempre tem estado presente na história da civilização. Também tem sido objeto de comentários a intenção de consolidá-la como uma ciência a presença de movimentos eugênicos em vários países do mundo, o holocausto nazista e, finalmente, o ressurgimento da eugenia na raíz da decodificação do genoma humano. Nosso objetivo é dar um repasso pelos movimentos eugênicos que tiveram lugar em meados do século XX, o ressurgimento da eugenia e os avanços com os quais contamos atualmente.Eugenics is a topic treated for several reasons by, among others, historians, philosophers, physicians, bioethicists. The idea to improve human race always has been present in the history of civilization, to try to consolidate it as a science also has been the object of commentaries, the presence of eugenic organizations in several countries, the nazi holocaust and finally, the eugenic resurgence since the human genome sequencing. Our objective is to review eugenic movements in the mid twentieth century, eugenic resurgence and current advancements.

Representações do discurso médico-eugênico sobre a descendência: a eugenia mendelista nas teses doutorais da Faculdade de Medicina e Cirurgia de São Paulo na década de 1920

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Beatriz Lopes Porto Verzolla

Full Text Available Resumo A eugenia representou um movimento de grande repercussão a partir do final do século XIX e pretendia o melhoramento e aprimoramento da espécie humana, abrangendo ações de educação e de restrições à reprodução, contribuindo para a construção da ordem e da civilidade, baseada no progresso e na superioridade moral e física dos indivíduos. Este artigo visa apresentar e discutir elementos extraídos das teses doutorais da Faculdade de Medicina da Universidade de São Paulo na década de 1920, especificamente relacionados ao tema da eugenia mendelista, sob a ótica da produção discente da época. O período escolhido representa um momento de grande influência da medicina na sociedade, em ações de controle e normatização dos indivíduos, influenciadas pelas teorias eugênicas. As teses doutorais representam o início da produção discente da Faculdade e contêm influências das teorias em voga no período - teorias evolucionistas, positivistas e eugenistas. Foram selecionadas para apresentação neste artigo oito teses doutorais, analisadas a partir do conceito de representações como técnica de leitura de documentos. Nas teses analisadas, pôde-se observar uma preocupação dos autores com a formação de uma descendência eugênica, defendendo o estabelecimento de medidas como restrição de casamentos, exame médico pré-nupcial e, em alguns casos, esterilização compulsória.

PROSPECÇÃO TECNOLÓGICA DE Eugenia uniflora L. (MYRTACEAE

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Adonias Almeida Carvalho

2016-06-01

Full Text Available Eugenia uniflora, pertencente à família Myrtaceae, é uma espécie arbórea, nativa do Bioma Mata Atlântica e conhecida no Brasil como pitangueira. É uma planta de frutos comestíveis, o chá das folhas tem aplicação na medicina popular como hipotensor, antigota, estomáquico e hipoglicemiante. Explorada pelas indústrias alimentícias, cosméticas e medicinais, é alvo de contínuos estudos em relação à composição química do óleo essencial. Este trabalho é uma prospecção e tem como objetivos mapear as pesquisas patenteadas referentes à pitangueira. A prospecção foi realizada com base nos pedidos de patentes depositadas no European Patent Office (EPO, na World Intellectual Property Organization (WIPO, no United States Patent and Trademark Office (USPTO e no Instituto Nacional de Propriedade Industrial do Brasil (INPI com coleta de dados nos meses de agosto e setembro de 2015. USPTO foi a base de dados que apresentou o maior número de pedidos patentes e o INPI apresentou um número pouco expressivo envolvendo a E. uniflora. No período de 2006 a 2015 observou-se, de modo geral, um aumento no número de pedido de patentes, verificou-se ainda que os Estados Unidos é o maior detentor de patentes, com destaque para as seções A (Necessidades Humanas e C (Química; Metalurgia da classificação internacional de patentes.

Evaluation of the Cytoprotective and Antioxidant Activity of the Extracts of Eugenia Uniflora Lineau e Psidium Sobraleanum Proença & Landrum Against Heavy Metals

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Celestina E. Sobral-Souza

2014-09-01

Full Text Available Objective: Evaluation of antioxidant activity has been an important issue considering its importance in human health. Recent studies show that the use of plants in the form of juices or teas as sources of natural antioxidants with low risk can be used as an aid to the treatment of various diseases. Material and Methods: Evaluation of the antioxidant potential in vitro, extracts of Eugenia uniflora and Psidium sobraleanum, as well as the quantification of phenols and flavonoids present in the extracts. Results: Findings showed a better antioxidant activity for the extract of Eugenia uniflora. In the TBARS test with egg phospholipids, extracts presented a reduction in the basal levels in the lipid peroxidation process; and when the Fe2 + extract was inducted, Psidium sobraleanum proved to be more efficient.. Conclusions: These tests proved that the extracts of leaves of the species Eugenia uniflora and Psidium sobraleanum present antioxidant activity which is directly related to phenolic substances produced in its secondary metabolism.

Initial growth of Eugenia stipitata, Inga spectabilis, and Inga edulis in Napo, Ecuador

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Ricardo Vinicio Abril Saltos

2018-05-01

Full Text Available Agriculture has been one of the causes of deforestation, causing environmental impact and soil degeneration, which leads to lower income earned by farmers; hence the need to implement agroforestry systems .This research aims to describe fromthe initial growth of Eugenia stipitata, Inga edulis, and Inga spectabilis to 320 days after emergence. The study took place at the Amazon Research and Conservation Center of the Amazonas State University, during 2014 and 2015. The growth process was evaluated in regards to plant height, stem diameter, number of leaves, and branch length. Plant height and stem diameter were compared over time applying variance analysis. Precipitation was also compared to analyze whether its variations had a direct influence on plant growth. The linear and polynomial models fitted better for thespecies regarding plant height and sprout diameter. Predominance of green leaves compared to yellow and dry ones, as branch length showed significant differences over all the sampling periods. The two-way analysis of variance showed significant differences in the correlation between age and precipitation over plant height and stem diameter. The outcomes led to conclude that Eugenia stipitata had a lower growth than both Inga edulis and Inga spectabilis. Branch production and growth and the number of leaves were also important elements of the growth process.

Armazenamento de sementes de cerejas-do-mato (Eugenia involucrata DC. submetidas ao recobrimento com biofilmes e embalagem a vácuo

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Alexandre Luis Alegretti

2015-02-01

Full Text Available Fruteiras como Eugenia involucrata ainda continuam inexploradas, necessitando-se de informações técnicas que incentivem o agricultor a utilizá-las. O método de propagação por sementes é o normalmente adotado; porém, elas devem ser imediatamente semeadas, pois corre-se o risco de perda de sua viabilidade com o armazenamento. O objetivo deste trabalho foi avaliar o hidrocondicionamento e as técnicas de conservação (vácuo e biofilme, durante armazenamento, aos cinco e aos 30 dias, de sementes de cerejeira-do-mato. O trabalho foi realizado na Universidade Tecnológica Federal do Paraná - Câmpus Dois Vizinhos. O delineamento experimental foi em blocos ao acaso, em fatorial 2 × 4 × 2 (pré-hidrocondicionamento x técnica de armazenamento x tempo de armazenamento, com quatro repetições, de 50 sementes por unidade experimental. As sementes extraídas foram separadas em dois lotes, sendo um submetido ao pré-hidrocondicionamento, em água destilada, durante 24 horas, e, outro, não. Sementes hidrocondicionadas, ou não, foram submetidas a quatro técnicas de armazenamento, sendo, estas, a embalagem plástica a vácuo, o revestimento com biofilme de fécula de mandioca (3% m/v, a embalagem plástica a vácuo + biofilme de fécula de mandioca e sem tratamento (controle. Posteriormente, as sementes foram mantidas em câmara fria, em temperatura de 5 ºC e UR 85%, durante cinco e 30 dias. Aos 110 dias após a semeadura, avaliou-se a percentagem de germinação, o índice de velocidade de emergência e a massa da matéria seca total das plântulas. Para o armazenamento das sementes de cerejeira-do-mato, devem-se utilizar técnicas a vácuo, isoladamente, ou com revestimento de biofilme.

Wound Healing Activity and Chemical Standardization of Eugenia pruniformis Cambess

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de Albuquerque, Ricardo Diego Duarte Galhardo; Perini, Jamila Alessandra; Machado, Daniel Escorsim; Angeli-Gamba, Thaís; Esteves, Ricardo dos Santos; Santos, Marcelo Guerra; Oliveira, Adriana Passos; Rocha, Leandro

2016-01-01

Background: Eugenia pruniformis is an endemic species from Brazil. Eugenia genus has flavonoids as one of the remarkable chemical classes which are related to the improvement of the healing process. Aims: To evaluate of wound healing activity of E. pruniformis leaves and to identify and quantify its main flavonoids compounds. Materials And Methods: Wound excision model in rats was used to verify the hydroethanolic and ethyl acetate extracts potential. The animals were divided in four groups of six and the samples were evaluated until the 15° day of treatment. Hydroxyproline dosage and histological staining with hematoxilin-eosin and Sirius Red were used to observe the tissue organization and quantify the collagen deposition, respectively. Chemical compounds of the ethyl acetate extract were identified by chromatographic techniques and mass spectrometry analysis and total flavonoids content was determined by spectrophotometric method. The antioxidant activity was determined by oxygen radical absorbing capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazylhydrate radical photometric (DPPH) assays. Results: The treated group with the ethyl acetate extract showed collagen deposition increase, higher levels of hidroxyproline, better tissue reorganization and complete remodeling of epidermis. Quercetin, kaempferol and hyperoside were identified as main compounds and flavonoids content value was 43% (w/w). The ORAC value of the ethyl acetate extract was 0.81± 0.05 mmol TE/g whereas the concentration to produce 50% reduction of the DPPH was 7.05± 0.09 μg/mL. Conclusion: The data indicate a wound healing and antioxidant activities of E. pruniformis. This study is the first report of flavonoids and wound healing activity of E. pruniformis. KEY MESSAGES Eugenia pruniformis extract accelerates wound healing in skin rat model, probably due to its involvement with the collagen deposition increase, higher levels of hidroxyproline, dermal remodelling and potent antioxidant activity

Atividade antioxidante de três espécies de Eugenia (Myrtaceae)

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Magina, Michele A.; Gilioli, Andressa; Moresco, Henrique H.; Colla, Guilherme; Pizzolatti, Moacir Geraldo; Brighente, Inês Maria Costa

2010-01-01

A atividade antioxidante dos extratos vegetais de Eugenia brasiliensis, E. beaurepaireana e E. umbelliflora foi avaliada usando diferentes métodos: Determinação da ação sequestrante de radicais livres usando DPPH, determinação do potencial redutor e determinação do potencial inibidor da peroxidação lipídica. Foi correlacionado também a o conteúdo de fenólicos totais e de flavonóides contidos nestes extratos e frações com a atividade antioxidante. As espécies E. brasiliensis e E. beaurepairean...

Preliminary pharmacological studies on Eugenia uniflora leaves: xanthine oxidase inhibitory activity.

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Schmeda-Hirschmann, G; Theoduloz, C; Franco, L; Ferro, E; de Arias, A R

1987-11-01

Eugenia uniflora is widely used in Paraguayan folk medicine. A hydroalcoholic extract of the leaves showed some central nervous system activity in hippocratic screening when given intraperitoneally, but little to no acute or subacute toxicity in doses up to 4200 mg/kg orally in BALB c mice. The LD50 of the extract was 220 mg/kg i.p. in mice. A decoction or infusion of the leaves is recommended for treating gout by native herbalists. The known flavonoids quercitrin, quercetin, myricitrin and myricetin were found to be responsible for the xanthine oxidase inhibitory action of the plant extract.

Ultraviolet-B Protective Effect of Flavonoids from Eugenia caryophylata on Human Dermal Fibroblast Cells

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Patwardhan, Juilee; Bhatt, Purvi

2015-01-01

Background: The exposure of skin to ultraviolet-B (UV-B) radiations leads to deoxyribonucleic acid (DNA) damage and can induce production of free radicals which imbalance the redox status of the cell and lead to increased oxidative stress. Clove has been traditionally used for its analgesic, anti-inflammatory, anti-microbial, anti-viral, and antiseptic effects. Objective: To evaluate the UV-B protective activity of flavonoids from Eugenia caryophylata (clove) buds on human dermal fibroblast c...

Eugenia e casamento Eugenics and marriage

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Luzia Aurelia Castañeda

2003-12-01

Full Text Available Analisaremos algumas estratégias eugênicas para melhoramento da raça baseadas no controle de matrimônios. Para tanto, vamos buscar no contexto da Inglaterra vitoriana a preocupação com o casamento; tanto na obra de Thomas Malthus, quando interessado no crescimento populacional, como na de Francis Galton, quando propõe a eugenia como uma ciência do melhoramento das qualidades inatas da raça. Discutiremos também como tal medida de controle de matrimônios se deu no movimento eugênico brasileiro e sua influência na elaboração do Código Civil.This analysis of some eugenic strategies for "improving the race" through the control of marriages explores Victorian England's concern with matrimony as expressed in the works of Thomas Malthus, who was interested in population growth, and of Francis Galton, who proposed that eugenics be used as a science to "improve" the innate qualities of the race. The article also discusses how this matrimonial means of control was expressed within the Brazilian eugenics movement and how it influenced the drafting of the Brazilian Civil Code.

Comparison of antihyperlipidaemic activity of eugenia jambolana fruit with punica granatum fruit in diet induced hyperlipidaemic rats

International Nuclear Information System (INIS)

Bilal, R.; Rashid, M.; Latif, W.

2013-01-01

Objective: To compare the antihyperlipidemic effects of Eugenia Jambolana fruit pulp with Punica Granatum fruit in diet induced hyperlipidaemic rats at the same dose level. Methods: An experimental randomized control study was conducted on seventy five male albino rats over a period of 14 weeks in University of Health Sciences Lahore. They were divided into five groups labelled A, B, C, D and E with fifteen rats in each group. Group A was kept as normal control, groups B, C, D and E were given hyperlipidaemic diet for six weeks. In group B no further intervention was done, group C and D were given ethanolic extract of Eugenia Jambolana and Punica Granatum respectively for eight weeks. Group E was given combination of both for same duration. Serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-c), lowdensity lipoprotein cholesterol (LDL-c) and triglycerides (TG) were measured at zero, six and fourteen weeks. Results: At fourteenth week significant reductions in TC, LDL-c and TG and a rise in HDL-c was observed in interventional groups C, D and E as compared to experimental hyperlipidaemic control group B (p 0.57, p > 0.22, p > 0.56, p > 0.76, respectively. On sixth week, there was no significant difference between groups B, C, D and E (p > 0.05). However, 15 rats of group A had significant lower levels of cholesterol, high density lipoproteins, low density lipoproteins and triglycerides when compared to 60 rats of groups B, C, D and E (p<0.05). Conclusion: In male albino rats combination of ethanolic extracts of Eugenia Jambolana and Punica Granatum fruit pulps was most effective in lowering serum total cholesterol and triglycerides while decrease in low density lipoprotein cholesterol and rise in high density lipoprotein cholesterol was same as the extracts given alone. (author)

Enhancement of the antifungal activity of antimicrobial drugs by Eugenia uniflora L.

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Santos, Karla K A; Matias, Edinardo F F; Tintino, Saulo R; Souza, Celestina E S; Braga, Maria F B M; Guedes, Gláucia M M; Costa, José G M; Menezes, Irwin R A; Coutinho, Henrique Douglas Melo

2013-07-01

Candidiasis is the most frequent infection by opportunistic fungi such as Candida albicans, Candida tropicalis, and Candida krusei. Ethanol extract from Eugenia uniflora was assayed, for its antifungal activity, either alone or combined with four selected chemotherapeutic antimicrobial agents, including anphotericin B, mebendazole, nistatin, and metronidazole against these strains. The obtained results indicated that the association of the extract of E. uniflora to metronidazole showed a potential antifungal activity against C. tropicalis. However, no synergistic activity against the other strains was observed, as observed when the extract was associated with the other, not enhancing their antifungal activity.

Pharmacological basis for the empirical use of Eugenia uniflora L. (Myrtaceae) as antihypertensive.

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Consolini, A E; Baldini, O A; Amat, A G

1999-07-01

The rational basis for the use of Eugenia uniflora L. (Myrtaceae) as antihypertensive in Northeastern Argentina was assessed in normotensive rats. Intraperitoneal administration of the aqueous crude extract (ACE) decreased blood pressure (BP) of normotensive rats dose-dependently until 47.1 +/- 8.2% of control. The effective-dose 50 was 3.1 +/- 0.4 mg dried leaves/kg (d.l./kg) (yielding of ACE: 17% w/w). To determine the origin of hypotensive activity. Alpha-adrenergic antagonistic and vasorelaxant ACE activities were tested. The dose-response curve for phenylephrine on BP was inhibited non-competitively until 80% of its maximal effect (at 8 mg d.l. ACE/kg). Perfusion pressure (PP) of rat hindquarters (previously vasoconstricted by high-K+) was decreased by ACE in a concentration-dependent manner until -32.3 +/- 11.5% of tonic contraction at 1.2 g d.l. ACE/100 ml. In addition, A.C.E demonstrated diuretic activity at a dose (120 mg d.l./kg) higher than the hypotensive one. It was almost as potent as amiloride, but while amiloride induced loss of Na+ and saving of K+, ACE induced decrease in Na+ excretion. The results suggest that the empirical use of Eugenia uniflora L. (Myrtaceae) is mostly due to a hypotensive effect mediated by a direct vasodilating activity, and to a weak diuretic effect that could be related to an increase in renal blood flow.

CARACTERIZAÇÃO ANATÔMICA E HISTOQUÍMICA DAS FOLHAS DE EUGENIA UNIFLORA L.

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Rafaela Damasceno Sá

2016-10-01

Full Text Available Eugenia uniflora L., popularmente conhecida como pitangueira, é uma importante frutífera muito utilizada na medicina tradicional brasileira para o tratamento de diarreia e disenteria, gripe, parasitas intestinais, febre, hipertensão e como calmante e diurético. Devido ao seu amplo interesse popular e com o intuito de ampliar as informações farmacobotânicas da espécie, este trabalho tem como objetivo realizar a caracterização anatômica e histoquímica das folhas de E. uniflora. Foram confeccionadas e analisadas em microscópico de luz lâminas semipermanentes contendo secções transversais de pecíolo e lâmina foliar, além de secções paradérmicas de lâmina foliar. Realizou-se também testes histoquímicos em secções transversais de lâmina foliar A análise microscópica permitiu a identificação de estruturas importantes na diagnose da espécie: cavidades secretoras no pecíolo e na lâmina foliar; cristais prismáticos no floema do pecíolo e da lâmina foliar, bem como no mesofilo e nas células parenquimáticas e colenquimáticas da nervura central; drusas estão presentes apenas no parênquima do pecíolo e no mesofilo, parênquima e colênquima da lâmina foliar; presença de cutícula espessa no pecíolo e na lâmina foliar; feixes vasculares bicolaterais e mesofilo dorsiventral, com parênquima paliçádico bisseriado. Através da histoquímica evidenciou-se na lâmina foliar a presença de compostos fenólicos, taninos, lignina, compostos lipofílicos, óleos essenciais, triterpenos e esteroides e cristais de oxalato de cálcio. Os resultados apresentados são úteis para a identificação correta da espécie.

Etica do discurso e eugenia liberal: Jürgen Habermas e o futuro da natureza humana | The ethics of discourse and liberal eugenics: Jürgen Habermas and the future of human nature

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Aécio Amaral

2008-04-01

Full Text Available Resumo Em O Futuro da Natureza Humana, Jürgen Habermas afirma que os avanços recentes no campo das biotecnologias constituem um desafio para a ética do discurso nas Ciências Sociais. Por trás de sua crítica aos defensores da eugenia liberal reside o reconhecimento de que o Diagnóstico Genético Pré-Implantação potencialmente põe em cheque o papel exercido pela razão comunicativa na constituição de uma ética individual de auto-compreensão. A ‘ética da espécie’ proposta por Habermas como contraposição a esse fenômeno se nos apresenta como moralmente reativa, na medida em que sua crítica não alcança abarcar os aspectos metafísicos que estão no núcleo do discurso da eugenia liberal. O artigo é dividido em dois momentos: perceber como a recente intervenção de Habermas ecoa o motivo da alegada colonização do mundo-da-vida pela razão tecnológica, e demonstrar como a concepção de técnica que embasa seu relato o impede de divisar a crítica dos aspectos metafísicos da cultura genética contemporânea. Palavras-chave Jürgen Habermas; cultura genética; sociedade da informação; ciência e mundo-da-vida; ética do discurso Abstract In The Future of Human Nature, Jürgen Habermas recognizes that current advances in biotechnology are challenging discourse ethics in Social Sciences. Behind his fear of the possibility of liberal eugenics, lies the recognition that pre-implanted genetic diagnosis potentially puts into question the role played by communicative reason in the constitution of the individual’s ethics of self-understanding. The ethics of species proposed by Habermas sounds morally reactive, insofar as his critique does not manage to encompass the metaphysical features which are at the core of liberal eugenics discourse. This paper is divided into two moments: the current echoing in Habermas’ work of the motif of the alleged colonization of the lifeworld by technological reason, and a demonstration of

Fome, eugenia e constituição do campo da nutrição em Pernambuco: uma análise de Gilberto Freyre, Josué de Castro e Nelson Chaves Starvation, eugenics and the development of nutrition in Pernambuco according to Gilberto Freire's, Josué de Castro's and Nelson Chaves' analyses

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Francisco de Assis Guedes de Vasconcelos

2001-08-01

Full Text Available O objetivo deste artigo é examinar a contribuição dos estudos de Gilberto Freyre, Josué de Castro e Nelson Chaves, produzidos nas décadas de 1930 e 1940, para o processo de constituição do campo da nutrição em Pernambuco. O estudo procura demonstrar que as temáticas fome e eugenia constituem as categorias centrais que expressam os pontos de tensão e de comunhão das abordagens desses autores pernambucanos, pelo estatuto científico nesse campo do conhecimento.The purpose of this article is to examine the contribution of the studies Gilberto Freyre, Josué de Castro and Nelson Chaves produced for the development of the Pernambuco nutrition field, from 1930 to 1940. The article attempts to demonstrate that both subjects - starvation and eugenics - are the central categories that express tension and communion in these three authors' approaches to this field of knowledge.

Enxertia herbácea em Myrtaceae nativas do Rio Grande do Sul

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Daiane Silva Lattuada

2010-12-01

Full Text Available O objetivo deste trabalho foi testar a viabilidade da técnica de enxertia herbácea em frutíferas nativas da família Myrtaceae. O experimento foi realizado em casa de vegetação do Departamento de Horticultura e Silvicultura/ UFRGS, em Porto Alegre. Duas espécies de Myrtaceae nativas foram usadas, tanto como porta-enxerto quanto como enxerto, Eugenia uniflora (Pitangueira e E. involucrata (Cerejeira-do-rio-grande ou Cerejeira-do-mato, totalizando quatro combinações. O método de enxertia adotado foi por garfagem em fenda cheia, em ramos herbáceos, com diâmetro médio do porta-enxerto de 0,1cm, enquanto os ramos dos enxertos apresentavam diâmetro médio entre 0,08 a 0,1cm, para as duas espécies. As análises foram quinzenais e, após 70 dias, foram analisadas estatisticamente a pega e a altura média das brotações emitidas. O delineamento experimental foi o completamente casualizado, com quatro repetições, sendo cada unidade experimental composta de cinco enxertos. Os resultados indicaram pega de 60% na combinação Pitangueira-Pitangueira (porta enxerto - enxerto. As demais combinações mostraram-se ineficientes.

Anti-Trypanosoma cruzi and cytotoxic activities of Eugenia uniflora L.

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Santos, Karla K A; Matias, Edinardo F F; Tintino, Saulo R; Souza, Celestina E S; Braga, Maria F B M; Guedes, Gláucia M M; Rolón, Miriam; Vega, Celeste; de Arias, Antonieta Rojas; Costa, José G M; Menezes, Irwin R A; Coutinho, Henrique D M

2012-05-01

Chagas disease is caused by Trypanosoma cruzi, being considered a public health problem. An alternative to combat this pathogen is the use of natural products isolated from fruits such as Eugenia uniflora, a plant used by traditional communities as food and medicine due to its antimicrobial and biological activities. Ethanolic extract from E. uniflora was used to evaluate in vitro anti-epimastigote and cytotoxic activity. This is the first record of anti-Trypanosoma activity of E. uniflora, demonstrating that a concentration presenting 50% of activity (EC(50)) was 62.76 μg/mL. Minimum inhibitory concentration (MIC) was ≤ 1024 μg/mL. Our results indicate that E. uniflora could be a source of plant-derived natural products with anti-epimastigote activity with low toxicity. Copyright © 2012 Elsevier Inc. All rights reserved.

Nutrient Status of Vermicompost of Urban Green Waste Processed by Three Earthworm Species Eisenia fetida, Eudrilus eugeniae, and Perionyx excavatus

International Nuclear Information System (INIS)

Pattnaik, S.; Vikram, M.

2010-01-01

Major nutrient status of vermicompost of vegetable market waste (MW) and floral waste (FW) processed by three species of earthworms namely, Eudrilus eugeniae, Eisenia fetida, and Perionyx excavatus and its simple compost were assessed across different periods in relation to their respective initiative substrates. Their physical parameters temperature, moisture, ph, and electrical conductivity were also recorded. The nutrients nitrogen, phosphorus, potassium, calcium, and magnesium increased in the vermicompost and compost while the organic carbon, C/N and C/P ratios decreased as the composting process progressed from 0 to 15, 30, 45, and 60 days. The nutrient statuses of vermicomposts of all earthworm species produced from both the wastes were more than that of the compost and that of their respective substrates. Moreover, the vermicompost produced by E. eugeniae possessed higher nutrient contents than that of E. fetida, P. excavatus, and compost. The MW showed higher nutrient contents than the FW. Thus, vermicomposting is the paramount approach of nutrient recovery of urban green waste.

Rheological behavior of Brazilian Cherry (Eugenia uniflora L. pulp at pasteurization temperatures

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Alessandra Santos Lopes

2013-03-01

Full Text Available The rheological behavior of Brazilian Cherry (Eugenia uniflora L. pulp in the range of temperatures used for pasteurization (83 to 97 °C was studied. The results indicated that Brazilian Cherry pulp presented pseudoplastic behavior, and the Herschel-Bulkley model was considered more adequate to represent the rheological behavior of this pulp in the range of temperatures studied. The fluid behavior index (n varied in the range from 0.448 to 0.627. The effect of temperature on the apparent viscosity was described by an equation analogous to Arrhenius equation, and a decrease in apparent viscosity with an increase in temperature was observed.

Evaluation of sublethal toxicity of zinc and chromium in Eudrilus eugeniae using biochemical and reproductive parameters.

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Basha, P Mahaboob; Latha, V

2016-05-01

Zinc (Zn) and chromium (Cr) act as essential nutrients; however, they can be toxic at higher concentrations. In this study, earthworm species Eudrilus eugeniae was studied for its sensitivity to sublethal doses of Cr (8 ppm) and Zn (350 ppm) in terms of alterations occurred in oxidative stress indices and reproductive parameters in the testis. A significant (p food intake was observed on 7, 14 and 21 days of Cr toxicity; while increased rate was evident upon 7 days of Zn exposure. Changes evident in the rate of cocoon production (-39.54 and +38.63 %), hatchability (-77.85 and +30.0 %) highlight higher toxic potential of Cr than Zn, respectively. Moderate to severe vacuolization in spermatic follicles and higher incidence of tailless (+52.6 % in Cr and 20.8 % in Zn), and head bent (+18.8 % in Cr and 0 % in Zn) sperms were evident in Cr(VI) exposed worms emphasizing the higher vulnerability of E. eugeniae to Cr toxicity. A significant (p implied from results that excessive free radical production and inadequate antioxidant defenses have lead to morphological alterations in sperms which sequentially reduced the reproductive rate.

EFEITOS DO EXTRATO DE Eugenia uniflora SOBRE OS PARÂMETROS HEMATOLÓGICOS E PRESSÃO ARTERIAL DE RATOS HIPERTENSOS

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Noemia Aparecida Partelli Mariani

2017-01-01

Full Text Available A Eugenia uniflora é uma planta amplamente encontrada no Brasil, utilizada popularmente para tratar diversos distúrbios. O objetivo deste estudo foi avaliar os efeitos do extrato de suas folhas sobre parâmetros hematológicos e pressão arterial de ratos espontaneamente hipertensos (SHR. Os animais foram divididos em grupos controle e tratados com extrato nas doses de 100mg/kg e 200mg/kg. O tratamento foi diário, via gavagem, por 28 dias. O sangue dos animais foi coletado semanalmente, para a contagem global e diferencial de leucócitos e ao final do tratamento, os animais foram anestesiados e foi realizada cateterização da artéria carótida para medida de pressão arterial sistólica, diastólica, média e frequência cardíaca. O extrato não alterou os parâmetros hematológicos e não reduziu a frequência cardíaca dos SHR, porém observou-se uma tendência à redução. Ao passo que, diminuiu a pressão arterial sistólica, diastólica e média, sugerindo um efeito anti-hipertensivo.

Atividade leishmanicida in vitro de Eugenia uniflora e Momordica charantia

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Karla Katiúcia Alves Santos

2013-01-01

Full Text Available A Leishmaniose Tegumentar Americana no Brasil é causada por uma variedade de espécies de Leishmania e uma grande diversidade destes parasitas pode ser encontrada na Região Amazônica. Revisões recentes na quimioterapia de leishmaniose enfatizam as deficiências dos agentes terapêuticos atualmente disponíveis e mostram a necessidade urgente de novos candidatos. Uma alternativa para substituir esses medicamentos são extratos naturais de Eugenia uniflora e Momordica charantia. Foram preparados extratos etanólicos das folhas de E. uniflora e M. charantia. Para os testes in vitro de Leishmania brasiliensis foram utilizadas formas promastigotas. O ensaio de citotoxicidade foi realizado com linhagens de fibroblastos. Nossos resultados indicam que E. uniflora foi eficaz contra a cepa de parasita testada, representando uma fonte alternativa de produtos naturais com atividade contra L. brasiliensis.

EGG TRAY DAUN SALAM (Eugenia polyantha

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Isna Bekti Pertiwi

2017-02-01

Full Text Available Telur ayam ras merupakan salah satu makanan yang bergizi tinggi dengan rasa yang enak dan harga relatif murah serta dapat diolah menjadi berbagai macam produk makanan. Namun, telur ayam ras mentah memiliki sifat mudah menurun kualitasnya dan mudah rusak karena lama wak-tu simpan telur yang relatif pendek. Penelitian ini secara umum bertujuan untuk mengetahui pe-ngaruh penggunaan baki telur daun salam (Eugenia polyantha terhadap lama waktu simpan telur ayam ras mentah yang dicuci dan tidak dicuci, dan secara khusus bertujuan untuk menge-tahui pengaruh perbandingan komposisi 0 %, 20 % dan 40 % dari daun salam yang digunakan. Penelitian yang dilakukan adalah true experiment dengan rancangan post test only with control group. Sebagai kontrol adalah baki telur yang biasa dijumpai di pasaran. Di setiap baki telur, pa-da suhu kamar diletakkan masing-masing 10 butir telur yang tidak dicuci dan 10 butir telur yang dicuci sebelumnya. Pengamatan lama waktu simpan telur dilakukan selama dua puluh tujuh hari dengan melihat perubahan kondisi telur-telur tersebut. Data dianalisis menggunakan uji Anova satu jalan pada α 0,05; dan hasilnya menunjukkan bahwa baki telur dengan komposisi daun salam 40 % adalah yang paling efektif dalam memperpanjang lama waktu simpan telur ayam ras mentah yang dicuci. Nilai mean difference yan dihasilkan adalah 14.00000 dan nilai p lebih kecil dari 0,001.

Essential oil composition of fruit colour varieties of Eugenia brasiliensis Lam. Composição dos óleos essenciais de variedades de coloração de frutos de Eugenia brasiliensis Lam.

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Paulo Roberto H. Moreno

2007-08-01

Full Text Available Eugenia brasiliensis Lam. is a variable species concerning fruit colour, with three recognized varieties. However, the definition of varieties is not easy for Myrtaceae species and not widely accepted. Two fruit colour varieties (purple and yellow of E. brasiliensis had their essential oil composition analysed in order to give support to the existence of varieties for this species. Although, the major components in the leaf oil are the same monoterpenes for both varieties, alpha-pinene, beta-pinene and 1,8-cineol, the purple fruit variety accumulates more oxygenated sesquiterpenes (33.9% than the one with yellow fruits (3.8%. The major differences occurred in purple fruits that present as major components caryophyllene oxide (22.2% and alpha-cadinol (10.4%, not found in the leaf oil, and the yellow fruit oil presented a similar composition as observed for the leaves. These fruit colour varieties of E. brasilensis can be considered as two distinct chemotypes, since the sesquiterpene pathway is more operant in the purple variety than in the yellow one, in which monoterpenes are mainly accumulated.A espécie Eugenia brasiliensis Lam. apresenta a coloração dos frutos variável, sendo reconhecidas três variedades. Entretanto, a definição de variedades não é fácil para espécies de Myrtaceae e também não é amplamente aceita. Duas variedades de Eugenia brasiliensis, baseado na cor dos frutos (roxos e amarelos, tiveram a composição de seus óleos essenciais analisadas com a finalidade de obter indícios de variedade botânica para esta espécie. Embora, os componentes principais nos óleos das folhas fossem os mesmos monoterpenos para ambas as variedades, alfa-pineno, beta-pineno e 1,8-cineol, a variedade com frutos roxos acumulou maior quantidade de sesquiterpenos oxigenados (33,9% do que aquela com frutos amarelos (3,8%. As diferenças principais ocorreram nos frutos roxos que apresentaram como componente principal o óxido de cariofileno

Identification of microRNAs from Eugenia uniflora by high-throughput sequencing and bioinformatics analysis.

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Guzman, Frank; Almerão, Mauricio P; Körbes, Ana P; Loss-Morais, Guilherme; Margis, Rogerio

2012-01-01

microRNAs or miRNAs are small non-coding regulatory RNAs that play important functions in the regulation of gene expression at the post-transcriptional level by targeting mRNAs for degradation or inhibiting protein translation. Eugenia uniflora is a plant native to tropical America with pharmacological and ecological importance, and there have been no previous studies concerning its gene expression and regulation. To date, no miRNAs have been reported in Myrtaceae species. Small RNA and RNA-seq libraries were constructed to identify miRNAs and pre-miRNAs in Eugenia uniflora. Solexa technology was used to perform high throughput sequencing of the library, and the data obtained were analyzed using bioinformatics tools. From 14,489,131 small RNA clean reads, we obtained 1,852,722 mature miRNA sequences representing 45 conserved families that have been identified in other plant species. Further analysis using contigs assembled from RNA-seq allowed the prediction of secondary structures of 25 known and 17 novel pre-miRNAs. The expression of twenty-seven identified miRNAs was also validated using RT-PCR assays. Potential targets were predicted for the most abundant mature miRNAs in the identified pre-miRNAs based on sequence homology. This study is the first large scale identification of miRNAs and their potential targets from a species of the Myrtaceae family without genomic sequence resources. Our study provides more information about the evolutionary conservation of the regulatory network of miRNAs in plants and highlights species-specific miRNAs.

Análise tecidual e celular das brânquias de Oreochromis niloticus L. tratadas com extrato etanólico bruto e frações das folhas da pitanga (Eugenia uniflora L. - Myrtaceae Tissue and cell analysis of Oreochromis niloticus L. gill treated with crude ethanol extract and fractions from pitanga (Eugenia uniflora L. leaves Myrtaceae

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T.S. Fiuza

2011-01-01

Full Text Available Eugenia uniflora L. (Myrtaceae é uma planta que ocorre no bioma Cerrado e é utilizada popularmente no tratamento de diarréias, inflamações, hiperglicemia e hipertensão. Estudos prévios revelaram atividade antimicrobiana da E. uniflora in vitro. Tendo em vista o uso popular, este trabalho objetivou avaliar as possíveis atividades celulares e teciduais sistêmicas do extrato bruto e das frações das folhas dessa planta em brânquias de Oreochromis niloticus L. (tilápia nilótica. Para isso, o extrato etanólico e as frações das folhas dessa planta foram administrados no peixe, por via oral, adicionadas à ração. Após um período de 24 horas, os peixes foram sacrificados e o segundo arco branquial de cada peixe foi dissecado, fixado em formalina neutra, desidratado, incluído em parafina e cortado. Nas análises histológicas, utilizaram-se tricômico de Masson e hematoxilina e eosina (HE. Pelas análises qualitativas na microscopia de luz, concluiu-se que o extrato etanólico bruto e as frações das folhas da E. uniflora apresentaram efeito sistêmico nas tilápias nilóticas atingindo as brânquias. As ações tóxicas como destacamento e descamação do epitélio respiratório e hiperplasia das células do epitélio interlamelar, foram mais pronunciadas nas tilápias que ingeriram maiores concentrações. Este trabalho colaborou para identificar o efeito vasodilatador dessa planta, e contribuiu para estabelecer a tilápia nilótica como sistema-modelo para testes com princípios ativos de plantas. Espera-se, com esses testes, viabilizar o uso de plantas como medicamentos para tratamentos de peixes, a manutenção da saúde de animais em cultivo intensivo e extensivo, a partir do qual se possibilite emprego alternativo aos medicamentos sintéticos.Eugenia uniflora L. (Myrtaceae is a plant found in the Cerrado biome and traditionally used in the treatment of diarrheas, inflammations, hyperglycemia and hypertension. Previous studies

Nutrient Status of Vermicompost of Urban Green Waste Processed by Three Earthworm Species—Eisenia fetida, Eudrilus eugeniae, and Perionyx excavatus

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Swati Pattnaik

2010-01-01

Full Text Available Major nutrient status of vermicompost of vegetable market waste (MW and floral waste (FW processed by three species of earthworms namely, Eudrilus eugeniae, Eisenia fetida, and Perionyx excavatus and its simple compost were assessed across different periods in relation to their respective initiative substrates. Their physical parameters—temperature, moisture, pH, and electrical conductivity—were also recorded. The nutrients—nitrogen, phosphorus, potassium, calcium, and magnesium—increased in the vermicompost and compost while the organic carbon, C/N and C/P ratios decreased as the composting process progressed from 0 to 15, 30, 45, and 60 days. The nutrient statuses of vermicomposts of all earthworm species produced from both the wastes were more than that of the compost and that of their respective substrates. Moreover, the vermicompost produced by E. eugeniae possessed higher nutrient contents than that of E. fetida, P. excavatus, and compost. The MW showed higher nutrient contents than the FW. Thus, vermicomposting is the paramount approach of nutrient recovery of urban green waste.

Indução de fitoalexinas em cotilédones de soja em resposta a derivados de folhas de pitangueira Induction of phytoalexins in cotyledons of soybean in response to the derivatives of leaf surinan cherry

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Sergio Miguel Mazaro

2008-10-01

Full Text Available A demanda mundial por alimentos isentos de agrotóxicos tem impulsionado a pesquisa para a busca de métodos alternativos ao controle de patógenos em plantas. A ativação dos mecanismos de defesa com o uso de indutores vem demonstrando ser uma alternativa viável e promissora. O uso de extratos de plantas medicinais tem demonstrado capacidade para induzir a produção de fitoalexinas, como um mecanismo de defesa em plantas tratadas. O objetivo deste trabalho foi verificar o potencial da planta Eugenia uniflora L. (pitangueira em induzir fitoalexinas em cotilédones de soja (Glycine max. Os derivados de extrato alcoólico, infusão, maceração e decocção, obtidos de folhas de pitangueira, foram usados nas concentrações de 0,1; 1; 10 e 40%, além de óleo essencial. Água foi utilizada como controle e quitosana (1% como indutor de referência. Os preparados de pitangueira apresentaram capacidade de indução das fitoalexinas gliceolinas em cotilédones de soja, respondendo ao aumento das concentrações dos preparados. O óleo essencial apresentou destacável efeito na indução de fitoalexinas, sendo superior aos demais preparados. Quitosana induziu fitoalexinas em cotilédones de soja e pode ser utilizado em estudos similares como um indutor de referência.The worldwide demand for food without pesticides has stimulated the research on alternative methods to control pathogens in plants. The activation of defense mechanisms by inductors seems a viable and promising alternative. The use of medicinal plants extracts has demonstrated capacity to induce the production of phytoalexins, as a mechanism of defense in treated plants. The objective of this research was to verify the potential of Eugenia uniflora L. (surinan cherry to induce phytoalexins in cotyledons of soybean (Glycine max. The derivatives alcoholic extract, infusion, maceration and decoction, obtained of surinan cherry were used in the concentrations of 0.1; 1; 10 and 40%, beyond

Efecto del choque térmico de arazá (Eugenia stipitata Me Vaugh sobre la tolerancia al frío

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Carlos Eduardo Narváez Cuenca

2010-10-01

Full Text Available Los frutos de arazá (Eugenia stipitata fueron cosechados en su madurez fisiológica y calentados a 50 °C durante O, 10, 20 ó 30 min antes de ser refrigerados a 7 °C durante 14 días y maduración complementaria a 25 °C durante 3 días.

Biologia floral e da polinização de quatro espécies de Eugenia L. (Myrtaceae Floral and pollination biology of four species of Eugenia L. (Myrtaceae

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André Luiz Gomes da Silva

2007-03-01

Full Text Available Eugenia uniflora, E. punicifolia, E. neonitida e E. rotundifolia são espécies perenes, geralmente de porte arbustivo, que ocorrem na restinga do Parque Natural Municipal de Grumari, região oeste do Município do Rio de Janeiro. Possuem inflorescências racemosas com flores pediceladas inseridas nas axilas foliares. Suas flores são andróginas, polistêmones, do tipo Papaver, generalistas, com grãos de pólen como único recurso floral sendo enquadradas dentro da categoria de "flores-pólen". O estigma é seco, diminuto e formado por papilas delgadas. A antese é diurna, as flores duram apenas um dia e são visitadas por uma ampla variedade de insetos, incluindo himenópteros, dípteros, coleópteros e neurópteros, totalizando 29 espécies. As abelhas são os visitantes mais comuns e, dentre elas, Apis mellifera L., espécie introduzida pela ação humana, é a mais freqüente e abundante, sendo considerada o polinizador efetivo das espécies aqui estudadas. A floração é anual e em massa. E. uniflora floresce de agosto a outubro, com frutificação de setembro a novembro. E. neonitida floresce do final de agosto até início de dezembro, com frutificação de outubro até início de janeiro. E. punicifolia floresceu por duas vezes no ano de 2003, sendo a primeira florada entre os meses de junho e julho, frutificando no mês de agosto e a segunda, entre os meses de setembro e outubro, frutificando entre novembro e dezembro e no ano de 2004, floriu apenas no mês de agosto e frutificou entre outubro e dezembro. E. rotundifolia floresceu no mês de março, com frutificação de maio a junho.Eugenia uniflora, E. punicifolia, E. neonitida and E. rotundifolia are perennial species, usually shrubs, occurring in the restinga of Grumari Natural Municipal Park west of Rio de Janeiro. They have racemose inflorescences with pedicellate flowers inserted in the leaf axils. The flowers are androgynous, polystemonous, of the Papaver type, and

Chemical constituents and antioxidant activity of leaves and branches of Eugenia copacabanensis Kiaersk (Myrtaceae); Constituintes quimicos e atividade antioxidante de folhas e galhos de Eugenia copacabanensis Kiaersk (Myrtaceae)

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Carvalho Junior, Almir Ribeiro de; Gomes, Geovany Amorim; Ferreira, Rafaela Oliveira; Carvalho, Mario Geraldo de, E-mail: almirribeiro@ufrrj.br [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Instituto de Ciencias Exatas. Departamento de Quimica

2014-05-15

Phytochemical investigation of Eugenia copacabanensis allowed for the isolation and identification of following compounds: β-sitosterol, β-sitosterol-glucoside, eight triterpenes, (mixture of α- and β-amyrins, ursolic acid, 30-hydroxy-ursolic acid, betulin, friedelin, friedelan-3,4-lactone, and taraxerol), a mixture of three sesquiterpenes, (clovandiol, globulol, and viridiflorol), three flavonoids (kaempferol-3-O-β-D-rhamnoside, quercetin-3-O-α-L-arabinoside, and quercetin), and a mixture of four coumaroyl esters (octacosanyl, heptacosanyl, hexacosanyl, and tetracosanyl coumarates). The structures of these compounds were assigned based on comparison with literature data and spectroscopic analysis, including analysis by two-dimensional NMR techniques. Total phenolic content and total flavonoids were evaluated. Antioxidant activities of methanol extracts and fractions were measured by the 1,2-diphenyl-2-picryl-hidrazyl free radical scavenging assay. (author)

Chemical and biological study of essential oils from Eugenia pruniformis cambess., an endemic species from Brazilian Atlantic Forest

OpenAIRE

Albuquerque, Ricardo D.D.G.; Tietbohl, Luis A. C.; Fernandes, Caio P.; Couteiro, Pedro P.; Eiriz, Débora N.; Santos, Marcelo G.; Silva Filho, Moacélio V.; Alves, Gutemberg G.; Bachinski, Róber; Rocha, Leandro

2012-01-01

Eugenia pruniformis Cambess. is an endemic species from Brazilian Atlantic Forest. Essential oils from leaves and fruits from this species were obtained by hydrodistillation and analyzed by GCMS/CG-FID. In all, 25 compounds were identified, with predominance of sesquiterpene hydrocarbons in both plant parts. The major compounds were β-caryophyllene, bicyclogermacrene, germacrene D, δ- cadinene and α-copaene. Antioxidant activity was performed for essential oil from leaves using ORAC method, s...

FLORÍSTICA E ESTRUTURA EM ÁREA DE FLORESTA ESTACIONAL DECIDUAL NA REGIÃO DO ALTO URUGUAI, RS / FLORISTIC AND STRUCTURE IN AREA OF SEASONAL DECIDUOUS FOREST IN THE REGION OF THE ALTO URUGUAI, RS

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F. R. Lambrecht

2016-06-01

Full Text Available           O trabalho teve como objetivos realizar a análise florística das estruturas horizontal e vertical em um fragmento de Floresta Estacional Decidual (FED na região do Alto Uruguai, em Frederico Westphalen, RS, Brasil. Foi realizado um censo em uma área amostral de 10.000 m², subdividida em 100 subparcelas de 10x10 m. Realizou-se a mensuração e a identificação de todos os indivíduos de espécies arbóreas com circunferência a altura do peito (CAP≥ 31,4cm. Com os dados obtidos, foram realizados cálculos de densidade (D, frequência (F, dominância (Do, índice de valor de importância (IVI, índice de valor de cobertura (IVC e a estratificação dos indivíduos. O maior número de espécies concentrou-se na família Fabaceae (10 e o maior número de indivíduos na família Meliaceae (147 ou 25,3%. Trichilia claussenii C. DC. Nectandra megapotamica (Spreng. Mez, Achatocarpus praecox Griseb. e Eugenia rostrifolia D. Legrand representaram 38,28% da densidade relativa e Holocalyx balansae Micheli, Trichilia claussenii, Nectandra megapotamica, Cordia americana (L. Gottshling & J.E.Mill., Phytolacca dioica  L., Achatocarpus praecox, e Eugenia rostrifolia representaram 50,5% da dominância relativa. Considerando a estrutura vertical, as sete espécies mais frequentes apresentaram uma maior quantidade de indivíduos no estrato médio. Conclui-se que a área florestal encontra-se em estágio intermediário de sucessão.

Efficient, highly enantioselective synthesis of selina-1,3, 7(11)-trien-8-one, a major component of the essential oil of Eugenia uniflora.

Science.gov (United States)

Kanazawa, A; Patin, A; Greene, A E

2000-09-01

The first synthesis of selina-1,3,7(11)-trien-8-one (1), a major constituent of the essential oil from the leaves of Eugenia uniflora, has been accomplished, with excellent stereo- and regiocontrol, in eight steps and in 12% overall yield from the known octalone derivative 2a.

In vivo pretreatment of Eudrilus eugeniae powder attenuates β-adrenoceptor toxicity mediated by isoproterenol in rat model

OpenAIRE

Jaganathan Anitha; Kadarkarai Murugan; Akon Higuchi; Abdullah A. Alarfaj; Murugan A. Munusamy; Giovanni Benelli

2016-01-01

The present study was designed to discover the potential cardioprotective function of earthworm powder (EWP) extracted from Eudrilus eugeniae on isoproterenol (ISO)-induced myocardial infarction in male Wistar rats. The rats were divided into four groups, with six rats in each group. Certain rats were pretreated with EWP (200 mg/kg bwt) (Group III), and a myocardial infarction was then induced by subcutaneous injection of ISO (85 mg/kg bwt) (Group II). Oral pretreatment of 200 mg/kg bwt of EW...

O abajurú (Chrysobalanus icaco L. e Eugenia rotundifolia Casar. comercializado na cidade do Rio de Janeiro, Brasil

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Inês Machline Silva

Full Text Available Este artigo analisa as prováveis razões de introdução e comercialização de uma espécie de uso medicinal em um mercado popular urbano na cidade do Rio de Janeiro, Brasil - o Mercado de Madureira. Durante os anos de 2005 e 2006 aplicaram-se entrevistas semi-estruturadas a 15 erveiros obtendo-se o freelist das espécies consideradas como mais comercializadas (97 a partir do qual se calculou o índice de saliência, que para o abajurú (Eugenia rotundifolia Casar, foi elevado. A espécie conhecida na literatura e comercializada como abajurú é Chysobalanus icaco L., que apresenta propriedades hipoglicemiantes comprovadas por pesquisas farmacológicas e é utilizada pela população para este fim; no entanto, verificou-se, nesse mercado, a venda quase exclusiva de E. rotundifolia, com esse nome popular e mesma propriedade. Até o momento não existem dados farmacológicos para essa espécie. Ambas são nativas e ocorrem, predominantemente, nas restingas litorâneas do estado do Rio de Janeiro. A atribuição da atividade hipoglicemiante a E. rotundifolia pode indicar uma correlação, por parte dos erveiros, com a farmacologia de outras espécies de Myrtaceae. Questões relacionadas à fiscalização ambiental bem como desconhecimento e coleta equivocada podem também estar envolvidos nesse processo.

The determination of antioxidant activity of Brazil-cherry (Eugenia uniflora L.) leaves extract using FRAP method

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Kurniawati, Puji; Maulida, Ika Rahma; Muhaimin

2017-12-01

Brazil-cherry leaves (Eugenia uniflora L.) have antioxidant activity because they contain phenolic and flavonoids compounds. The aim of the study was to obtain antioxidant activity using FRAP (Ferric Reducing Antioxidant Power) method. Brazil-cherry leaves were extracted using soxhlet method by ethanol 70%. The free radical activity absorbance was measured with a UV-Vis spectrophotometer at a wavelength of 711 nm and the total value of antioxidant activity was calculated based on the data absorbance. The results showed that Brazil-cherry leaves have the antioxidant activity with the value 2.493mgAAE/g extract.

EBV DNA polymerase inhibition of tannins from Eugenia uniflora.

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Lee, M H; Chiou, J F; Yen, K Y; Yang, L L

2000-06-30

Nasopharyngeal carcinoma (NPC) is one of the high population malignant tumors among Chinese in southern China and southeast Asia. Epstein-Barr virus (EBV) is a human B lymphotropic herpes virus which is known to be closely associated with NPC. EBV DNA polymerase is a key enzyme during EBV replication and is measured by its radioactivity. The addition of phorbol 12-myristate 13-acetate to Raji cell cultures led to a large increase in EBV DNA polymerase, which was purified by sequential DEAE-cellulose, phosphocellulose and DNA-cellulose column chromatography. Four tannins were isolated from the active fractions of Eugenia uniflora L., which were tested for the inhibition of EBV DNA polymerase. The results showed the 50% inhibitory concentration (IC(50)) values of gallocatechin, oenothein B, eugeniflorins D(1) and D(2) were 26.5 62.3, 3.0 and 3.5 microM, respectively. Furthermore, when compared with the positive control (phosphonoacetic acid), an inhibitor of EBV replication, the IC(50) value was 16.4 microM. In view of the results, eugeniflorins D(1) and D(2) are the potency principles in the inhibition of EBV DNA polymerase from E. uniflora.

Lead contamination in Eugenia dyeriana herbal preparations from different commercial sources in Malaysia.

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Ang, H H

2008-06-01

The Drug Control Authority (DCA) of Malaysia implemented the phase three registration of traditional medicines on 1 January, 1992. A total of 100 products in various pharmaceutical dosage forms of a herbal preparation, containing Eugenia dyeriana, either single or combined preparations (more than one medicinal plant), were analyzed for the presence of lead contamination, using atomic absorption spectrophotometry. These samples were bought from different commercial sources in the Malaysian market, after performing a simple random sampling. Results showed that 22% of the above products failed to comply with the quality requirement for traditional medicines in Malaysia. Although this study showed that 78% of the products fully complied with the quality requirement for traditional medicines in Malaysia pertaining to lead, however, they cannot be assumed safe from lead contamination because of batch-to-batch inconsistency.

Essential oil of the leaves of Eugenia uniflora L.: antioxidant and antimicrobial properties.

Science.gov (United States)

Victoria, Francine Novack; Lenardão, Eder João; Savegnago, Lucielli; Perin, Gelson; Jacob, Raquel Guimarães; Alves, Diego; da Silva, Wladimir Padilha; da Motta, Amanda de Souza; Nascente, Patricia da Silva

2012-08-01

Essential oil (EO) of the leaves of Eugenia uniflora L. (Brazilian cherry tree) was evaluated for its antioxidant, antibacterial and antifungal properties. The acute toxicity of the EO administered by oral route was also evaluated in mice. The EO exhibited antioxidant activity in the DPPH, ABTS and FRAP assays and reduced lipid peroxidation in the kidney of mice. The EO also showed antimicrobial activity against two important pathogenic bacteria, Staphylococcus aureus and Listeria monocytogenes, and against two fungi of the Candida species, C. lipolytica and C. guilliermondii. Acute administration of the EO by the oral route did not cause lethality or toxicological effects in mice. These findings suggest that the EO of the leaves of E. uniflora may have the potential for use in the pharmaceutical industry. Copyright © 2012 Elsevier Ltd. All rights reserved.

Acute toxicity of chemical pesticides and plant-derived essential oil on the behavior and development of earthworms, Eudrilus eugeniae (Kinberg) and Eisenia fetida (Savigny).

Science.gov (United States)

Vasantha-Srinivasan, Prabhakaran; Senthil-Nathan, Sengottayan; Ponsankar, Athirstam; Thanigaivel, Annamalai; Chellappandian, Muthiah; Edwin, Edward-Sam; Selin-Rani, Selvaraj; Kalaivani, Kandaswamy; Hunter, Wayne B; Duraipandiyan, Veeramuthu; Al-Dhabi, Naif Abdullah

2018-04-01

Comparative toxicity of two chemical pesticides (temephos and monocrotophos) versus a plant-derived betel leaf oil Piper betle (L.) to earthworm Eudrilus eugeniae (Kinberg) and redworm Eisenia fetida Savigny, historically: Eisenia foetida (Savigny 1826), was evaluated. Mortality rate was more prominent in temephos at 100 μg concentration to both the earthworms in filter paper test (FPT) as well as 10 mg concentration in artificial soil test (AST). In contrast, P. betle does not display much mortality rate to both the earthworms even at 1000 mg of treatment concentrations. The lethal concentration (LC 50 ) value was observed at 3.89 and 5.26 mg/kg for temephos and monocrotophos against E. eugeniae and 3.81 and 5.25 mg/kg to E. fetida, respectively. Whereas, LC 50 value of betel leaf oil was only observed at 3149 and 4081 mg/kg to E. eugeniae and E. fetida, respectively. Correspondingly, the avoidance or attraction assay also displayed that earthworms were more sensitive to the soil containing chemical pesticides. Whereas, the avoidance percentage was decreased in the P. betle oil. Similarly, sublethal concentration of chemical pesticides (5 and 6.5 mg) significantly reduced the earthworm weight and growth rate. However, P. betle oil did not change the developmental rate in the duration of the assay (2, 7 and 14 days) even at 4000 mg treatment concentration. The enzyme ratio of CAT and SOD was also affected significantly after exposure to the chemical pesticides (6.5 mg/kg). Hence, our study implied the risk assessment associated with the chemical pesticides and also recommends plant-derived harmless P. betle oil against beneficial species as an alternative pest control agent.

Antioxidant Activities of Hydrolysable Tannins and Flavonoid Glycosides Isolated from Eugenia uniflora L.

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Gilmara A. C. Fortes

2015-01-01

Full Text Available Aphytochemical investigation of Eugenia uniflora ’s leaf extract resulted in the isolation of eleven phenolic compounds: 2,3-di-O-galloyl- b - D -glucose (1, 1,2,3,4,6-penta-O-galloyl- b - D -glucose (2, gemin D (3, hippomanin A (4, oenothein B (5, eugeniflorin D 2 (6, camptothin A (7, afzelin (8, quercitrin (9, myricitrin, (10 and desmanthin-1 (11. These compounds were identified by spectroscopic methods including 1D- and 2D-NMR, UV, IR, and TOF/MS. Compounds 1, 2, 3, 4, 7, 8, 9, and 11 were isolated from this species for the first time. Ten isolates were evaluated for antioxidant activity by DPPH free radical and Oxygen Radical Absorbance Capacity (ORAC-Fluorecein assay . Dimeric tannins, oenothein B (5, eugeniflorin D 2 (6, and camptothin A (7 showed a remarkable radical scavenging capacity.

Raciocínio moral na tomada de decisões em relação a questões sociocientíficas: o exemplo do melhoramento genético humano Moral reasoning in decision-making regarding socioscientific issues: the human genetic improvement example

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Márcio Andrei Guimarães

2010-01-01

Full Text Available Nos últimos anos, os avanços nas Ciências Biológicas têm levado a sociedade a discutir diversas questões no campo da moral e da ética. Questões como engenharia genética, clonagem e pesquisas com células-tronco são questões chamadas de sociocientíficas por estarem na interface entre a ciência e a sociedade. Nesse trabalho buscamos entender como estudantes de Ensino Médio percebem e interpretam questões relacionadas à manipulação genética em seres humanos. Houve divisão de opiniões em relação à eugenia negativa, que se destina a remover características desfavoráveis das pessoas; mas a eugenia positiva, que busca melhoramento de características estéticas, foi rejeitada por todos os estudantes. As variações nas opiniões em relação ao assunto tratado podem ser, em grande medida, devidas às representações sociais dos estudantes.In the recent years the advances in biological sciences has been convincing society to discuss issues concerning moral and ethics. Issues as cloning, stem-cell research and genetic engineering are called socioscientific issues because they put into evidence the interface between science and society. In this paper we aimed at understanding how High School students realize and interpret issues regarding human genetic improvement. As a result we have found divergent opinions regarding negative eugenics, specifically about its attempts of taking out undesirable characteristics of individuals; but there was an agreement among all the students in rejecting positive eugenics, that aims at improving individuals esthetical characteristics. The divergences and differences of opinions may be related to the students' social representations.

Enxertia herbácea em Myrtaceae nativas do Rio Grande do Sul Herbaceans grafiting in native Myrtaceae on Rio Grande do Sul

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Daiane Silva Lattuada

2010-12-01

Full Text Available O objetivo deste trabalho foi testar a viabilidade da técnica de enxertia herbácea em frutíferas nativas da família Myrtaceae. O experimento foi realizado em casa de vegetação do Departamento de Horticultura e Silvicultura/ UFRGS, em Porto Alegre. Duas espécies de Myrtaceae nativas foram usadas, tanto como porta-enxerto quanto como enxerto, Eugenia uniflora (Pitangueira e E. involucrata (Cerejeira-do-rio-grande ou Cerejeira-do-mato, totalizando quatro combinações. O método de enxertia adotado foi por garfagem em fenda cheia, em ramos herbáceos, com diâmetro médio do porta-enxerto de 0,1cm, enquanto os ramos dos enxertos apresentavam diâmetro médio entre 0,08 a 0,1cm, para as duas espécies. As análises foram quinzenais e, após 70 dias, foram analisadas estatisticamente a pega e a altura média das brotações emitidas. O delineamento experimental foi o completamente casualizado, com quatro repetições, sendo cada unidade experimental composta de cinco enxertos. Os resultados indicaram pega de 60% na combinação Pitangueira-Pitangueira (porta enxerto - enxerto. As demais combinações mostraram-se ineficientes.The objective of this study was to test the viability of the technique of green grafting in natives Myrtaceae. The experiment was conducted in greenhouse conditions of the Departamento de Horticultura e Silvicultura / UFRGS, in Porto Alegre. Two species of Myrtaceae were used, as well as rootstock and as graft, Eugenia uniflora (Surinam cherry and E. involucrata (Rio-grande-cherry or Cherry of the woods, totaling four combinations. The adopted grafting method consisted to cleft the graft on herbaceous branches, with an average diameter of the rootstock of 0.1 cm, while the branches of the grafts had an average diameter between 0.08 to 0.1 cm for both species. The observations were carried out every two weeks, and after 70 days were statiscally analyzed. The experimental design was completely randomized with four

Chemical constituents and antioxidant activity of leaves and branches of Eugenia copacabanensis Kiaersk (Myrtaceae)

International Nuclear Information System (INIS)

Carvalho Junior, Almir Ribeiro de; Gomes, Geovany Amorim; Ferreira, Rafaela Oliveira; Carvalho, Mario Geraldo de

2014-01-01

Phytochemical investigation of Eugenia copacabanensis allowed for the isolation and identification of following compounds: β-sitosterol, β-sitosterol-glucoside, eight triterpenes, (mixture of α- and β-amyrins, ursolic acid, 30-hydroxy-ursolic acid, betulin, friedelin, friedelan-3,4-lactone, and taraxerol), a mixture of three sesquiterpenes, (clovandiol, globulol, and viridiflorol), three flavonoids (kaempferol-3-O-β-D-rhamnoside, quercetin-3-O-α-L-arabinoside, and quercetin), and a mixture of four coumaroyl esters (octacosanyl, heptacosanyl, hexacosanyl, and tetracosanyl coumarates). The structures of these compounds were assigned based on comparison with literature data and spectroscopic analysis, including analysis by two-dimensional NMR techniques. Total phenolic content and total flavonoids were evaluated. Antioxidant activities of methanol extracts and fractions were measured by the 1,2-diphenyl-2-picryl-hidrazyl free radical scavenging assay. (author)

Availability and use of woody plants in a agrarian reform settlement in the cerrado of the state of Goiás, Brazil

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Vanessa Pessanha Tunholi

2013-09-01

Full Text Available Protected areas are important sources providing plant resources for human populations, and contributing to the livelihood and income of families. To test the ecological apparency hypothesis, that there is positive relationship between the visibility of a species and its use, we conducted an ethnobotanical survey in the protected area of the Itaúna rural settlement in the municipality of Planaltina, in the state of Goiás, in central Brazil. The phytosociological parameters were obtained in 5.6 ha area of the Brazilian cerrado (savanna, and the ethnobotanical survey conducted with semi-structured interviews with 75 people. There was a weak positive correlation between the use value and the phytosociological parameters. A principal component analysis showed that seven species (Pterodon pubescens, Hancornia speciosa, Hymenaea stigonocarpa, Eugenia dysenterica, Annona crassiflora, Stryphnodendron adstringens and Solanum lycocarpum have high use value, despite having low density, dominance and frequency. Nineteen species showed a positive relationship between their use value and their phytosociological parameters. The relationship between vegetation structure and use value differed among species in the protected area, indicating that more attention should be paid to those under more pressure, with low abundance. Further studies of these seven species should be conducted to determine whether human pressure is affecting populations structures and to define management strategies.

Arboreal component, phytosociology structure and environmental relations in a remnant of cerradão, Curvelo - MG

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Thiago José Ornelas Otoni

2013-06-01

Full Text Available This work aimed to verify the existence of variations in composition and distribution of tree species due to soil characteristics in a fragment of dystrophic savanna in Curvelo-MG. On description in the compartment of the phanerophytes of this plant community, were installed ten permanent plots with 1.000 m2 (20 x 50 m with fixed distances of 100 m between each plot. All alive individuals that had diameter at soil height (DSH - diameter to 0.3 m from ground level > 5.0 cm were sampled. The Individuals with multiple boles were sampled when the value of square root of the sum-square of DSH had been greater than criterion of the inventory. Were collected Soil samples at two depths (0-20 e 20-40 cm and was measured the distances of plots of a watercourse. To study the community were generated ordination diagrams of plots, species and environmental variables by means of a canonical correspondence analysis (CCA. The richness sampled was 92 species, 36 families and 74 genera, Erythroxylum, Byrsonima, Myrcia and Qualea. Jackknife estimators (first and second order designed a wealth of 106.4 and 107.8 species. The species Magonia pubescens, Terminalia argentea, Annona crassiflora, Eugenia dysenterica e Xylopia aromatica were distribuited underneath the environmental gradient.

Biologia da mosca‑das‑frutas sul‑americana em frutos de mirtilo, amoreira‑preta, araçazeiro e pitangueira Biology of South American fruit fly in blueberry, blackberry, strawberry guava, and Surinam cherry crops

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Maicon Bisognin

2013-02-01

Full Text Available O objetivo deste trabalho foi descrever a biologia de Anastrepha fraterculus em frutos de mirtilo (Vaccinium ashei, amoreira‑preta (Rubus spp., araçazeiro (Psidium cattleyanum e pitangueira (Eugenia uniflora. O experimento foi realizado em laboratório, em condições controladas de temperatura (25±2ºC, umidade relativa (70±10% e fotófase (12 horas, para determinação dos parâmetros biológicos do inseto nos estágios de desenvolvimento imaturos e adultos. Anastrepha fraterculus completa o ciclo biológico em todos hospedeiros estudados, embora os frutos nativos (pitanga e araçá ofereçam melhores condições para seu desenvolvimento. Os parâmetros biológicos determinados para as fases imaturas foram semelhantes nos quatro hospedeiros. Insetos criados em pitanga e araçá apresentam, na fase adulta, maior período de oviposição, fecundidade e longevidade de fêmeas, em comparação aos criados em mirtilo e amora‑preta. O ritmo diário de oviposição é mais prolongado e uniforme nos insetos criados em araçá e pitanga, o que mostra que A. fraterculus está mais bem adaptada a estas frutas, nativas da região Sul.The objective of this work was to describe the biology of Anastrepha fraterculus in blueberry (Vaccinium ashei, blackberry (Rubus spp., strawberry guava (Psidium cattleyanum and Surinam cherry (Eugenia uniflora fruits. The experiment was carried out in laboratory under controlled conditions of temperature (25±2ºC, relative humidity (70±10%, and photophase (12 hours to determine insect biological parameters in immature and adult development stages. Anastrepha fraterculus finishes its biological cycle in all studied hosts; however, the Brazilian native fruits (strawberry guava and Surinam cherry provide better conditions for development of the insect. Biological parameters determined for immature development stadium were similar in the four hosts. Insects reared in Surinam cherry and strawberry guava showed, in the

A study on the effect of chemically synthesized magnetite nanoparticles on earthworm: Eudrilus eugeniae

Science.gov (United States)

Samrot, Antony V.; Justin, C.; Padmanaban, S.; Burman, Ujjala

2017-02-01

Most look into the benefits of the nanoparticles, but keeping aside the benefits; this study focuses on the impacts of nanoparticles on living systems. Improper disposal of nanoparticles into the environment is a subject of pollution or nano-pollution which in turn affects the flora and fauna in the ecosystem, particularly soil ecosystem. Thus, this study was done to understand the impacts of chemically synthesized magnetite nanoparticles on earthworm— Eudrilus eugeniae, a soil-dependent organism which acquires food and nutrition from decaying matters. The chemically synthesized magnetite nanoparticles were characterized by UV-visible spectrophotometry, Fourier transform infrared spectroscopy and field emission scanning electron microscopy. Earthworms were allowed to interact with different concentrations of synthesized nanoparticles and the effect of the nanoparticles was analysed by studying the phenotypic changes followed by histology and inductively coupled plasma optical emission spectrometry analyses.

Electrolytic treatment and biosurfactants applied to the conservation of Eugenia uniflora fruit

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Guilherme DILARRI

2016-01-01

Full Text Available Abstract Microorganisms are the primary responsible for food poisoning and food spoilage. The purpose of this study was to evaluate different fruit washing methods with tap water, electrolyzed water and rhamnolipids solution produced by Pseudomonas aeruginosa LBI, in order to inhibit microbial growth. The tested organism was Eugenia uniflora. The fruits were washed and periodically inoculated into culture media to evaluate and count the colonies on the fruit surface. It was also observed the deterioration level of the fruits after each treatment. The results showed that treatment with rhamnolipids were the most efficient, inhibiting the growth of fungi and bacteria. The electrolyzed water proved to be very efficient in bacterial inhibition at the initial time, but in the final time it did not present any inhibitory effect. The electrolyzed water was also not effective in eliminating fungus. Washing with tap water was the less efficient treatment of all. The only treatment that showed an increased durability has been with rhamnolipids, increasing shelf life by up to two days. Thus rhamnolipids are the most recommended method for fruits sanitation.

ESTUDO FITOQUÍMICO E ANTIMICROBIANO DAS FOLHAS DE EUGENIA ASTRINGENS CAMBESS. (MYRTACEAE

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Bruna Carminate

2017-02-01

Full Text Available O conhecimento sobre plantas medicinais simboliza muitas vezes o único recurso terapêutico de muitas comunidades e grupos étnicos. A tradição popular é a origem de valiosos conhecimentos acerca das plantas, muitas das observações populares sobre a utilização de plantas medicinais contribuem, de forma relevante, para a divulgação das virtudes terapêuticas vegetais. A E. astringens é uma espécie que apresenta casca e folhas aromáticas, adstringentes, anti-reumáticas e diuréticas. Apesar do uso medicinal já relatado, não existem muitos estudos quanto a atividade antimicrobiana. O objetivo do presente trabalho foi a realização da triagem fitoquímica e a avaliação da atividade antimicrobiana do extrato hidroalcoólico das folhas de Eugenia astringens Cambess. Foram encontrados taninos, fenóis, catequinas e ácidos orgânicos e o extrato apresentou atividade antibacteriana para todas as 8 cepas testadas.

In-vitro evaluation of anti-trichomonal activities of Eugenia uniflora leaf.

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Ibikunle, Gabriel Femi; Adebajo, Adeleke Clement; Famuyiwa, Funmilayo Gladys; Aladesanmi, Adetunji Joseph; Adewunmi, Clement Oladapo

2011-01-01

Eugenia uniflora, used ethnomedically in some tropical countries as an anti-infective, has shown anti-malarial and anti-trypanocidal activities. Therefore using bioactivity guided fractionation, anti-trichomonal activity of E. uniflora leaf was investigated. Anti-trichomonal activities of leaf methanol extract and its fractions against Trichomonas gallinae as well as their cytotoxicities using an in vitro haemaglutination assay were determined. Anti-trichomonacidal activities of the extract improved on purification up to a stage. Subfractions E(2-5) had LC(50) and LC(90) values of 4.77 - 5.28, 18.49 - 25.00 and 4.53 - 5.18, 18.32 - 19.07 µg/ml at 24 and 48 hrs, respectively that were better than those of metronidazole. Further purification of E(2-5) led to loss of activity suggesting that the active components were probably working synergistically and additively. Demonstration of low haemaglutination titre values of 0.00 - 5.33 by methanolic extract and its partition fractions suggested their low toxicity profile. The established safety of the leaf indicated that its anti-trichomonal activity was not due to non-specific cytotoxicity, hence could be used in ethnomedicine as an anti-trichomonal agent.

AVALIAÇÃO DA ATIVIDADE LEISHMANICIDA IN VITRO DE EUGENIA UNIFLORA

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Elaine Gimenez Guimarães

2016-12-01

Full Text Available As leishmanioses são um conjunto de doenças causadas por protozoários do gênero Leishmania, transmitidas por meio de hábitos hematófagos de fêmeas de dípteros infectados, pertencentes à subfamília Phlebotominae. É uma doença negligenciada e de grande relevância devido sua incidência e letalidade quando não tratada. O tratamento das leishmanioses é um obstáculo, devido à alta toxidade e efeitos adversos causados pelos fármacos disponíveis. Neste sentido, as plantas medicinais têm ganhado grande destaque na busca por fármacos que possam apresentar menos efeitos colaterais. Diante disso, este trabalho teve por objetivo avaliar atividade leishmanicida do extrato hidroalcoólico das folhas de Eugenia uniflora na espécie Leishmania amazonensis. Os valores de IC50 foram de 1,99 µg/mL, 1,64 µg/mL e 1,39 µg/mL nos tempos de 24, 48 e 72 horas, respectivamente. Os resultados demonstram que o extrato testado possui significativa atividade leishmanicida.

Physicochemical characterization and antioxidant capacity of pitanga fruits (Eugenia uniflora L.

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Milena Bagetti

2011-03-01

Full Text Available This study was carried out to obtain more information about the physicochemical properties, composition, and antioxidant activity of pitanga fruits (Eugenia uniflora L., particularly fruits from the State of Rio Grande do Sul, Brazil. Pitanga with different flesh colors (purple, red, and orange from tree selections cultivated at Embrapa Clima Temperado (RS-Brazil were analyzed. Only slight differences were observed in the quality parameters and in the proximate and fatty acid compositions among the fruits studied. The extracts from purple-fleshed pitanga had the highest total phenolic and anthocyanin contents along with the highest antioxidant capacity. The antioxidant capacity (DPPH and FRAP assays of methanolic pitanga extracts was highly correlated with the total phenolic content, but in ethanolic extracts, the anthocyanin content was correlated only with the FRAP antioxidant capacity. Orange fleshed pitanga had higher β-cryptoxanthin and β-carotene levels than those of the red fruit, which had higher lycopene content. The results indicate that the purple-fleshed pitanga, cultivated in Rio Grande do Sul, is a rich source of phenolic compounds and has high antioxidant capacity. The red and orange-fleshed pitanga, on the other hand, are rich sources of carotenoids.

a-glucosidase Inhibitors From Paraguayan Natural Medicine, Ñangapiry, The Leaves Of Eugenia Uniflora.

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Matsumura, T; Kasai, M; Hayashi, T; Arisawa, M; Momose, Y; Arai, I; Amagaya, S; Komatsu, Y

2000-01-01

The water-soluble extract from a Paraguayan natural medicine, Nangapiry, the leaves of Eugenia uniflora L. (Myrtaceae), which has been used as an antidiabetic agent, was found to show inhibitory activities on the increase of plasma glucose level in the sucrose tolerance test (STT) conducted with mice. The portion adsorbed on a cation exchange resin was also found to inhibit a-glucosidases. From the active portion, two new active compounds named uniflorines A ( 1 ) and B ( 2 ) and known (+)-(3a, 4a, 5ß)-1-methylpiperidine-3, 4, 5-triol ( 3 ) were isolated. The structures of uniflorines A and B were determined as (-)-(1S, 2R, 6S, 7R, 8R, 8aR)-1,2,6,7,8-pentahydroxyindolizidine and (+)-(1S, 2R, 5R, 7R, 8S, 8aS)-1,2,5,7,8-pentahydroxyindolizidine by spectral means, respectively.

Fitossociologia de cerrado sensu stricto no município de Abaeté-MG Phytosociology of the cerrado sensu stricto in Abaeté, MG, Brazil

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Amilcar Walter Saporetti Jr

2003-06-01

Full Text Available O cerrado tem sido objeto de discussão de grupos temáticos que estudam a conservação de biodiversidade no Estado de Minas Gerais. São inúmeras as áreas de conservação com vegetação de cerrado das quais não se têm informações a respeito de sua composição florística e estrutura. O objetivo deste trabalho foi avaliar florística e fitossociologicamente uma área de cerrado sensu stricto, no município de Abaeté-MG. A área de estudo é um fragmento com 2 ha de cerrado sensu stricto, preservado como área de reserva da CAF-Santa Bárbara, situada nas coordenadas 19º05'S e 44º58'W, a uma altitude de 480 m, em leve depressão próxima de uma vereda. O clima pertence ao tipo Cwa pelo sistema de Köppen, com precipitação média anual de 1.400 mm. O solo é do tipo Latossolo Vermelho distrófico. Foram instaladas 15 parcelas de 200 m² (10 x 20 m, distribuídas sistematicamente ao longo de trilhas, distanciadas 10 m entre si. Foram amostrados todos os indivíduos lenhosos vivos com circunferência do caule ao nível do solo (CAS igual ou maior que 10 cm. O índice de Shannon foi de 3,590 e a equabilidade foi de 0,804, considerados comuns para cerrados bem conservados. Foram amostrados 1.339 indivíduos, sendo a composição florística constituída por 85 espécies, distribuídas em 44 famílias. As famílias com maior número de espécies foram Leguminosae Caesalpinioideae com sete espécies, Annonaceae com cinco, Myrtaceae, Malpighiaceae, Erythroxylaceae, Anacardiaceae, Rubiaceae e Bignoniaceae com quatro, seguidas de Vochysiaceae e Leguminosae Papilionoideae com três. As espécies que apresentaram o maior valor de importância (VI foram Xylopia aromatica (Lam. Mart. (22,21, Myrcia lingua Berg (18,18 Caryocar brasiliense Cambess. (17,91, Eugenia dysenterica DC. (17,58, Byrsonima intermedia A. Juss. (13,69 e Brosimum gaudichaudii Trécul (11,86.The cerrado has been a topic of discussion of thematic groups studying biodiversity

Eugenia uniflora L. Essential Oil as a Potential Anti-Leishmania Agent: Effects on Leishmania amazonensis and Possible Mechanisms of Action

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Amorim, Layane Valéria; de Oliveira, Jamylla Mirck Guerra; Dias, Clarice Noleto; Moraes, Denise Fernandes Coutinho; Andrade, Eloisa Helena de Aguiar; Maia, Jose Guilherme Soares; Carneiro, Sabrina Maria Portela; Carvalho, Fernando Aécio de Amorim

2013-01-01

Eugenia uniflora L. is a member of the Myrtaceae family and is commonly known as Brazilian cherry tree. In this study, we evaluated the chemical composition of Eugenia uniflora L. essential oil (EuEO) by using gas chromatography-mass spectrometry (GC-MS) and assessed its anti-Leishmania activity. We also explored the potential mechanisms of action and cytotoxicity of EuEO. Thirty-two compounds were identified, which constituted 92.65% of the total oil composition. The most abundant components were sesquiterpenes (91.92%), with curzerene (47.3%), γ-elemene (14.25%), and trans-β-elemenone (10.4%) being the major constituents. The bioactivity shown by EuEO against promastigotes (IC50, 3.04 μg·mL−1) and amastigotes (IC50, 1.92 μg·mL−1) suggested significant anti-Leishmania activity. In the cytotoxicity determination, EuEO was 20 times more toxic to amastigotes than to macrophages. Hemolytic activity was 63.22% at the highest concentration tested (400 μg·mL−1); however, there appeared to be no toxicity at 50 μg·mL−1. While the data show that EuEO activity is not mediated by nitric oxide production, they do suggest that macrophage activation may be involved in EuEO anti-Leishmania activity, as evidenced by increases in both the phagocytic capacity and the lysosomal activity. More studies are needed to determine in vivo activity as well as additional mechanisms of the anti-Leishmania activity. PMID:23533469

Eugenia uniflora L. Essential Oil as a Potential Anti-Leishmania Agent: Effects on Leishmania amazonensis and Possible Mechanisms of Action

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Klinger Antonio da Franca Rodrigues

2013-01-01

Full Text Available Eugenia uniflora L. is a member of the Myrtaceae family and is commonly known as Brazilian cherry tree. In this study, we evaluated the chemical composition of Eugenia uniflora L. essential oil (EuEO by using gas chromatography-mass spectrometry (GC-MS and assessed its anti-Leishmania activity. We also explored the potential mechanisms of action and cytotoxicity of EuEO. Thirty-two compounds were identified, which constituted 92.65% of the total oil composition. The most abundant components were sesquiterpenes (91.92%, with curzerene (47.3%, γ-elemene (14.25%, and trans-β-elemenone (10.4% being the major constituents. The bioactivity shown by EuEO against promastigotes (IC50, 3.04 μg·mL−1 and amastigotes (IC50, 1.92 μg·mL−1 suggested significant anti-Leishmania activity. In the cytotoxicity determination, EuEO was 20 times more toxic to amastigotes than to macrophages. Hemolytic activity was 63.22% at the highest concentration tested (400 μg·mL−1; however, there appeared to be no toxicity at 50 μg·mL−1. While the data show that EuEO activity is not mediated by nitric oxide production, they do suggest that macrophage activation may be involved in EuEO anti-Leishmania activity, as evidenced by increases in both the phagocytic capacity and the lysosomal activity. More studies are needed to determine in vivo activity as well as additional mechanisms of the anti-Leishmania activity.

Eugenia uniflora L. Essential Oil as a Potential Anti-Leishmania Agent: Effects on Leishmania amazonensis and Possible Mechanisms of Action.

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Rodrigues, Klinger Antonio da Franca; Amorim, Layane Valéria; de Oliveira, Jamylla Mirck Guerra; Dias, Clarice Noleto; Moraes, Denise Fernandes Coutinho; Andrade, Eloisa Helena de Aguiar; Maia, Jose Guilherme Soares; Carneiro, Sabrina Maria Portela; Carvalho, Fernando Aécio de Amorim

2013-01-01

Eugenia uniflora L. is a member of the Myrtaceae family and is commonly known as Brazilian cherry tree. In this study, we evaluated the chemical composition of Eugenia uniflora L. essential oil (EuEO) by using gas chromatography-mass spectrometry (GC-MS) and assessed its anti-Leishmania activity. We also explored the potential mechanisms of action and cytotoxicity of EuEO. Thirty-two compounds were identified, which constituted 92.65% of the total oil composition. The most abundant components were sesquiterpenes (91.92%), with curzerene (47.3%), γ -elemene (14.25%), and trans- β -elemenone (10.4%) being the major constituents. The bioactivity shown by EuEO against promastigotes (IC50, 3.04  μ g·mL(-1)) and amastigotes (IC50, 1.92  μ g·mL(-1)) suggested significant anti-Leishmania activity. In the cytotoxicity determination, EuEO was 20 times more toxic to amastigotes than to macrophages. Hemolytic activity was 63.22% at the highest concentration tested (400  μ g·mL(-1)); however, there appeared to be no toxicity at 50  μ g·mL(-1). While the data show that EuEO activity is not mediated by nitric oxide production, they do suggest that macrophage activation may be involved in EuEO anti-Leishmania activity, as evidenced by increases in both the phagocytic capacity and the lysosomal activity. More studies are needed to determine in vivo activity as well as additional mechanisms of the anti-Leishmania activity.

Constituintes químicos e atividade antioxidante de folhas e galhos de Eugenia copacabanensis Kiaersk (Myrtaceae

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Almir Ribeiro de Carvalho Junior

2014-06-01

Full Text Available Phytochemical investigation of Eugenia copacabanensis allowed for the isolation and identification of following compounds: β-sitosterol, β-sitosterol-glucoside, eight triterpenes, (mixture of α- and β-amyrins, ursolic acid, 30-hydroxy-ursolic acid, betulin, friedelin, friedelan-3,4-lactone, and taraxerol, a mixture of three sesquiterpenes, (clovandiol, globulol, and viridiflorol, three flavonoids (kaempferol-3-O-β-D-rhamnoside, quercetin-3-O-α-L-arabinoside, and quercetin, and a mixture of four coumaroyl esters (octacosanyl, heptacosanyl, hexacosanyl, and tetracosanyl coumarates. The structures of these compounds were assigned based on comparison with literature data and spectroscopic analysis, including analysis by two-dimensional NMR techniques. Total phenolic content and total flavonoids were evaluated. Antioxidant activities of methanol extracts and fractions were measured by the 1,2-diphenyl-2-picryl-hidrazyl free radical scavenging assay.

Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

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Rattmann, Yanna D; de Souza, Lauro Mera; Malquevicz-Paiva, Simone M; Dartora, Nessana; Sassaki, Guilherme Lanzi; Gorin, Philip A J; Iacomini, Marcello

2012-01-01

Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

Record of Edessa scabriventris Stål (Hemiptera: Pentatomidae) associated to Eugenia uniflora (Brazilian-Cherry) and Psidium guajava (Guava) (Myrtaceae), in north-northeastern Brazil.

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Lima, Mauricio S; Fernandes, José A M; Lima, Iracilda M M

2010-01-01

This study reports for the first time Edessa scabriventris Stål on Eugenia uniflora (Brazilian-cherry) and on Psidium guajava (guava) (Myrtaceae), fruit trees with economic value. Its geographic distribution is extended with records for the states of Alagoas (Maceió Municipality 35°45'11.16''W; 9°40'18.52''S) and Pará (Belém Municipality 48°28'14.65''W; 1°26'14.83''S), north-northeastern Brazil.

Eugenia negativa e positiva: significados e contradições Eugenesia negativa y positiva: significados y contradicciones Negative and positive eugenics: meanings and contradictions

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Lilian Denise Mai

2006-04-01

Full Text Available A prática da eugenia constitui importante tema de debate associado aos atuais avanços biogenéticos. Considerando que a centralidade da eugenia é a preocupação com a saúde e constituição das futuras gerações, e que a utilização de meios e conhecimentos científicos em prol do nascimento de uma criança física e mentalmente saudável pode ser considerada uma ação eugênica, pretende-se, nesse texto, analisar significados e contradições das ações eugenistas negativas e positivas, construídos concomitantes aos avanços técnico-científicos do século XX. Conclui-se que os significados transitam, respectivamente, em torno de limitar ou estimular a reprodução humana, no início do século, até prevenir doenças ou melhorar características físicas e mentais, na atualidade. Implementando ações, produziram-se contradições, como a discriminação e eliminação de muitas pessoas frente a um ideal de homem, à biologização de fatores eminentemente sociais, à defesa da pretensa neutralidade científica e ao uso indiscriminado do direito de escolha reprodutiva.La práctica de la eugenesia es un importante tema de debate, ligado a los avances biogenéticos actuales. Considerando que la cuestión central de la eugenesia es la preocupación con la salud y la constitución de las futuras generaciones y que la utilización de medios y conocimientos científicos en función del nacimiento de un niño física y mentalmente saludable puede ser considerada una acción eugenésica, en este texto se pretende analizar los significados y contradicciones de las acciones eugenésicas negativas y positivas, construidos concomitantes a los avances técnico-científicos del siglo XX. Se concluye que los significados giran, respectivamente, en torno de limitar o estimular la reproducción humana, al comienzo del siglo, hasta prevenir enfermedades o mejorar características físicas y mentales, en la actualidad. Implementando acciones, se

Augmentation of catecholamine release elicited by an Eugenia punicifolia extract in chromaffin cells

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Ricardo de Pascual

2011-10-01

Full Text Available Plant extracts of Eugenia punicifolia (Kunth DC., Myrtaceae, are used in Amazon region of Brazil to treat diarrhea and stomach disturbances, and as hypoglycemic medicine. We have recently shown that an aqueous extract of E. punicifolia augmented cholinergic neurotransmission in a rat phrenic nerve-diaphragm preparation. In this study, we investigated the effects of an E. punicifolia dichloromethane extract (EPEX in a neuronal model of cholinergic neurotransmission, the bovine adrenal chromaffin cell. EPEX augmented the release of catecholamine triggered by acetylcholine (ACh pulses but did not enhance ACh-evoked inward currents, which were inhibited by 30%. Since EPEX did not cause a blockade of acetylcholinesterase or butyrylcholinesterase, it seems that EPEX is not directly activating the cholinergic system. EPEX also augmented K+-elicited secretion without enhancing the whole-cell inward calcium current. This novel and potent effect of EPEX in enhancing exocytosis might help to identify the active component responsible for augmenting exocytosis. When elucidated, the molecular structure of this active principle could serve as a template to synthesise novel compounds to regulate the exocytotic release of neurotransmitters.

Bioactive Formylated Flavonoids from Eugenia rigida: Isolation, Synthesis, and X-ray Crystallography.

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Zaki, Mohamed A; Nanayakkara, N P Dhammika; Hetta, Mona H; Jacob, Melissa R; Khan, Shabana I; Mohammed, Rabab; Ibrahim, Mohamed A; Samoylenko, Volodymyr; Coleman, Christina; Fronczek, Frank R; Ferreira, Daneel; Muhammad, Ilias

2016-09-23

Two new flavonoids, rac-6-formyl-5,7-dihydroxyflavanone (1) and 2',6'-dihydroxy-4'-methoxy-3'-methylchalcone (2), together with five known derivatives, rac-8-formyl-5,7-dihydroxyflavanone (3), 4',6'-dihydroxy-2'-methoxy-3'-methyldihydrochalcone (4), rac-7-hydroxy-5-methoxy-6-methylflavanone (5), 3'-formyl-2',4',6'-trihydroxy-5'-methyldihydrochalcone (6), and 3'-formyl-2',4',6'-trihydroxydihydrochalcone (7), were isolated from the leaves of Eugenia rigida. The individual (S)- and (R)-enantiomers of 1 and 3, together with the corresponding formylated flavones 8 (6-formyl-5,7-dihydroxyflavone) and 9 (8-formyl-5,7-dihydroxyflavone), as well as 2',4',6'-trihydroxychalcone (10), 3'-formyl-2',4',6'-trihydroxychalcone (11), and the corresponding 3'-formyl-2',4',6'-trihydroxydihydrochalcone (7) and 2',4',6'-trihydroxydihydrochalcone (12), were synthesized. The structures of the isolated and synthetic compounds were established via NMR, HRESIMS, and electronic circular dichroism data. In addition, the structures of 3, 5, and 8 were confirmed by single-crystal X-ray diffraction crystallography. The isolated and synthetic flavonoids were evaluated for their antimicrobial and cytotoxic activities against a panel of microorganisms and solid tumor cell lines.

Chromatographic profiles of extractives from leaves of Eugenia uniflora

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Isabelle C.F. Bezerra

Full Text Available ABSTRACT Eugenia uniflora L., Myrtaceae, popularly known as “pitanga”, is used in traditional medicine due the properties attributed to its chemical content, these being mainly hydrolysable tannins and flavonoids. This study provides a qualitative and quantitative evaluation of chemical profile from leaves of E. uniflora. The HPLC analysis was carried out on a C18 column (4.6 mm × 250 mm, 5 µm by gradient elution with methanol and water (acidified with trifluoracetic acid; and silica gel Plates 60-F 254 with 10–12 µm and 5–6 µm particles, respectively for TLC and High HPTLC analysis. The chromatographic data obtained from HPLC, TLC and HPTLC presented bands and peaks related to flavonoids (myricitrin and derivatives and tannins (gallic and ellagic acids, which were observed from different samples. The chromatographic similarities enabled the building of a typical fingerprint for the herbal material. The similarity analysis of the sample data by Pearson correlation showed R values >0.9 among peaks (HPLC and bands (HPTLC. In addition, the analytical methodology developed by HPLC enabled the satisfactory quantification of marker substances [ellagic acid = 0.22% and 0.20% (m/m; gallic acid = 0.20% and 0.43%; myricitrin = 0.42 and 1.74% (m/m in herbal drug and crude extract, respectively]. The procedure was also validated in accordance with the assays required by Brazilian legislation. Thus, the HPTLC and HPLC methods developed in this study provide helpful and simple tools for the quality evaluation both qualitatively and quantitatively of raw materials and extractives from leaves of E. uniflora.

The sublethal effects of the organochlorines dieldrin and lindane on growth and reproduction of Eudrilus eugeniae and Eisenia fetida (Oligochaeta

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Editorial Office

1994-07-01

Full Text Available Experimental exposure of the earthworm species Eudrilus eugeniae to organochlorines showed that dieldrin causes damage to sperm ultrastructure when viewed electronmicroscopically. Worms containing concentrations of 7,27 mg/kg dieldrin and higher showed more than 10% sperm damage. Exposure of Eisenia fetida to sublethal concentrations of lindane did not result in sperm damage but demonstrated an increase in growth and reproductive activity. It is argued that quantification of sperm damage and correlation with pesticide concentration could provide a useful tool for evaluating environmental quality. Furthermore, the effects of sublethal concentrations of pesticides that manifest themselves in increased growth and reproductive activity could affect ecological balances.

Extração de DNA genômico de tecidos foliares maduros de espécies nativas do cerrado

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Márcia Nara da Silva

2010-12-01

Full Text Available Grandes quantidades de contaminantes na amostra de DNA dificultam a obtenção de DNA genômico de qualidade durante a extração. A presença de polissacarídeos, fenóis e outros compostos secundários representa o principal problema com o procedimento de isolamento do DNA e sua aplicação subsequente, por inibir a atividade das enzimas Taq DNA polimera-se e enzimas de restrição. Neste estudo, descreveu-se um procedimento modificado baseado no hexadecyltrimethylammonium (CTAB, rendendo DNA genômico satisfatório para técnicas de manipulação subsequente, como reações de PCR e digestão com enzima de restrição. Nesse protocolo foram utilizadas diferentes concentrações de β-mercaptoetanol no tampão de extração (0,0; 0,2; 10; 15; 25; e 50 uL de β-mercaptoetanol/mL do tampão de extração: 100 mM de Tris-HCl, pH 8; 20 mM de EDTA; 1,4 mM de NaCl; 2% de CTAB; 1% de PVP, cujo procedimento foi aplicado no caso de folhas maduras e testado em Annona crassiflora (arati-cum, Eugenia dysenterica (cagaita, Anacardium humilis (caju-do-campo, Hancornia speciosa (mangaba e Caryocar brasiliense (pequi. O protocolo foi eficiente no isolamento de DNA livre de polissacarídeos e polifenóis, com rendimento do DNA com alto peso molecu-lar, utilizando-se concentrações a partir de 1% de β-mercaptoetanol no tampão de extração. O DNA isolado por esse método mostrou alta pureza, de acordo com as análises de digestão por restrição e amplificação por PCR.

ENSAYO PRELIMINAR DE LA ACTIVIDAD ANTIBACTERIANA DE EXTRACTOS DE ALLIUM SATIVUM, CORIANDRUM SATIVUM, EUGENIA CARYOPHYLLATA, ORIGANUM VULGARE, ROSMARINUS OFFICINALIS Y THYMUS VULGARIS FRENTE A CLOSTRIDIUM PERFRINGENS

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Ardila Q., Martha I; Vargas A., Andrés F; Pérez C., Jorge E; Mejía G., Luis F

2009-01-01

Se evaluó la actividad antibacteriana frente a Clostridium perfringens (cepa ATCC: 13124) por el método de Kirby Bauer en agar SPS de los aceites esenciales o extractos vegetales obtenidos con solventes orgánicos de diferente polaridad a partir de Allium sativum (ajo), Coriandrum sativum (cilantro), Eugenia Caryophyllata (clavo de olor), Origanum vulgare (orégano), Rosmarinus officinalis (romero) y Thymus vulgaris (tomillo), utilizando la vancomicina como control. Los extractos obtenidos por ...

Microencapsulation of Eugenia uniflora L. juice by spray drying using fructans with different degrees of polymerisation.

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Ortiz-Basurto, R I; Rubio-Ibarra, M E; Ragazzo-Sanchez, J A; Beristain, C I; Jiménez-Fernández, M

2017-11-01

The objective of this work was to microencapsulate pitanga (Eugenia uniflora L.) juice by spray drying, using High Performance Agave Fructans (HPAF) and High Degree of Polymerisation Agave Fructans (HDPAF) and maltodextrin (MD), respectively, as the wall materials. The physicochemical and antioxidant properties of the capsules during storage at various temperatures were evaluated. The microparticles developed using fructans HPAF and HDPAF, exhibited similar physicochemical and flow properties to those presented by the microparticles prepared with MD. The highest yield and concentration of anthocyanins after drying and during storage were found for a 1:6 core:wall material ratio. The total color change was a good indicator of the microcapsule stability. This study showed that both fructans fraction possess similar encapsulating properties to MD and that the HDPAF were more efficacious than MD at protecting the antioxidants during drying and storage. Copyright © 2017 Elsevier Ltd. All rights reserved.

María Eugenia Dengo Obregón: mujer de lectura, educación y cultura...

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Jóse Pablo Eduarte Salazar

2008-01-01

Full Text Available El artículo es una semblanza de la Licenciada María Eugenia Dengo Obregón viuda de Vargas que recorre la vida y las obras de esta dama en su faceta personal, profesional, pública y más recien-temente, como exponente de la cultura costarricense. Este documento es un sentido homenaje a la figura de esta incansable mujer, que con su lectura y escritura ha logrado dejar un legado en la educación costarri-cense, como profesora, decana, vicerrectora, ministra y ex ministra de educación pública. La Licda. Dengo Obregón fue galardonada con el premio nacional de Cultura “Magón 2007” otorgado por el Ministerio de Cultura y Juventud de la República de Costa Rica a sus 82 años de edad. Honor al que honor merece.

Antifungal activity and ultrastructural alterations in Pseudocercospora griseola treated with essential oils Atividade antifúngica e alterações ultraestruturais em Pseudocercospora griseola tratado com óleos essenciais

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Julián Mauricio Ágredo Hoyos

2012-06-01

Full Text Available Pseudocercospora griseola, the etiologic agent of angular leaf spot of common bean (Phaseolus vulgaris, is an important disease in all bean-producing regions worldwide and may cause extremely high yield losses. The control of this disease is made more difficult by the pathogen's genetic variability and the inefficiency of fungicides. In this study, of 26 essential oils tested at different concentrations, 25 demonstrated efficiency in affecting the germination of strains 63-31 and 63-63 of the pathogen, reaching inhibition levels of between 80% and 100%. Cymbopogon citratus and Cymbopogon martinii inhibited conidia germination at all concentrations; Eugenia caryophyllata, Cinnamomum sp., Thymus vulgaris, Matricaria recutita, Cordia verbenacea, Origanum vulgare, Cymbopogon nardus, at 0.1 and 0.5%; and Zingiber officinale, Mentha arvensis, Chamaecyparis pisifera, Lavandula officinalis, Ocimum basilicum, Pimpinella anisum, Ocimum selloi, Baccharis dracunculifolia, Laurus nobilis, Citrus sinensis, Melaleuca alternifolia and Eucalyptus globulus, at 0.5%. The main constituents identified were cinnamaldehyde in Cinnamomum sp.; eugenol in E. caryophyllata; trans-β-farnesene in M. recutita; pulegone in C. verbenacea; thymol in T. vulgaris; geranial and neral in C. citratus, and geraniol in C. martini. Through transmission electron microscopy (TEM, it was verified that C. citratus, C. martini and E. caryophyllata presented direct fungitoxic action on P. griseola, causing severe damage to the cellular ultrastructure of the conidia, invalidating germination. These results indicated that essential oils are a promising alternative strategy for the control of angular leaf spot in bean, representing less risk to human health and the environment.Pseudocercospora griseola, agente etiológico da mancha angular do feijoeiro comum (Phaseolus vulgaris, é uma doença importante nas regiões produtoras de feijão em todo o mundo e pode causar perdas de produtividade

Dr Eugenia Rose Aylmer Cooper (1898-1991): Manchester's renowned female anatomist and neurohistologist.

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Shreeve, David R

2016-11-01

Having excelled in histology, Dr Eugenia Cooper, following graduation in medicine in Manchester, embarked on a career spanning 44 years in anatomy and histology at Manchester University. Her inimitable character was readily remembered by those she had taught. She was the first female graduate to gain an MD with gold medal for her thesis on the histology of the endocrine organs. However, her main study was the development of the human brainstem from the early weeks of gestation, which remains the basis for anatomical understanding today. More controversial was her theory on circulation and absorption of the cerebrospinal fluid. On retiring as Reader in Histology, she expressed disappointment at not being appointed a professor, which she considered was due to her gender. Possibly to compensate for this, she had studied law as an additional interest. She continued in research for a further 10 years in reproductive pharmacology. After retirement she donated her medals to the University, three to be awarded in medicine and histology, which have now lapsed, but the medals in computer science and music continue to be important rewards. © The Author(s) 2014.

Influence of different extraction methods on the yield and linalool content of the extracts of Eugenia uniflora L.

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Galhiane, Mário S; Rissato, Sandra R; Chierice, Gilberto O; Almeida, Marcos V; Silva, Letícia C

2006-09-15

This work has been developed using a sylvestral fruit tree, native to the Brazilian forest, the Eugenia uniflora L., one of the Mirtaceae family. The main goal of the analytical study was focused on extraction methods themselves. The method development pointed to the Clevenger extraction as the best yield in relation to SFE and Soxhlet. The SFE method presented a good yield but showed a big amount of components in the final extract, demonstrating low selectivity. The essential oil extracted was analyzed by GC/FID showing a large range of polarity and boiling point compounds, where linalool, a widely used compound, was identified. Furthermore, an analytical solid phase extraction method was used to clean it up and obtain separated classes of compounds that were fractionated and studied by GC/FID and GC/MS.

Influence of foliar nutrients on phenol levels in leaves of Eugenia uniflora

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Rosa M. Santos

2011-08-01

Full Text Available Eugenia uniflora L., Myrtaceae, leaves contain high amounts of phenolic compounds which are responsible for several pharmacological activities. In order to evaluate the phenolics seasonal variation leaves were analysed on a monthly basis during the period of two years for the contents of hydrolysable tannins, total phenols, flavonoids, and nutrients (N, P, K, S, Ca, Mg, Mn, Zn, Cu, and Fe. Results were correlated with climate conditions (rainfall, humidity, and mean temperature by Principal Component and ClusterAnalysis which allowed four groups to be distinguished with respect to the age of the leaves and the content of some metals. Young leaves were characterised by high levels of Zn and nitrogen whereas old leaves contained high levels of Fe and calcium, and both groups had moderate amounts of phenolics. Adult leaves were divided in two groups and results revealed that while one group had the highest levels of all phenols and lowest amounts of Mn and Cu, the other showed opposite quantities. The Canonical Correlation Analysis confirmed a highly significant negative correlation between phenol contents and Mn and Cu. These facts suggested that flavonoids and tannins production depends of the amounts of foliar nutrients, Cu and Mn in particular, which are cofactors of enzymes involved in phenol degradation and lignin biosynthesis. This knowledge can improve this specie cultivation in order to enhance the phenolic compounds concentration.

Pharmacological effects of Eugenia uniflora (Myrtaceae) aqueous crude extract on rat's heart.

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Consolini, Alicia E; Sarubbio, Marisol Gracía

2002-06-01

The effect of aqueous crude extract (ACE) of Eugenia uniflora L. (Myrtaceae) was studied on rat's perfused ventricles. This plant is used in South American traditional medicine as an antihypertensive and we already demonstrated previously its hypotensive properties. In this paper, maximal left intraventriclular pressure (P) of rat's hearts beating at 0.2 Hz firstly increased to 162.1+/-11.1% of basal value during 1-3 min of perfusing ACE 0.6%. Maximum rate of contraction (+P) also increased to duplicating +P/P ratio. Both types of effect were significantly decreased by either propranolol 0.35 microM, and pre-treatment with reserpine (5 mg/kg), suggesting that they were caused by a compound that releases cathecolamines with beta-adrenergic action. Nevertheless, after 20 min of perfusing ACE, ventricles decreased P to about 50% of their basal value, suggesting a negative-inotropic compound present in the extract. The perfusion of 1.2% ACE decreased P in a pressure-[Ca](o) curve (0.5-2 mM) in a non-competitive manner, suggesting that an irreversible Ca-blocking compound is also present in the extract. In summary, E. uniflora ACE has a dual effect on the heart related to its hypotensive action and is probably responsible for the therapeutic or adverse effects in patients under cardiac risk.

Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells.

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Yang, L L; Lee, C Y; Yen, K Y

2000-08-31

Eugenia jambos L. (Myrtaceae) is an antipyretic and anti-inflammatory herb of Asian folk medicine. A 70% acetone extract exerted the strongest cytotoxic effects on human leukemia cells (HL-60) from a preliminary screening of 15 plants. The cytotoxic principles were separated by bio-assay-guided fractionation to HL-60 cells; two hydrolyzable tannins (1-O-galloyl castalagin and casuarinin) were isolated from the 70% acetone extract. All significantly inhibited human promyelocytic leukemia cell line HL-60 and showed less cytotoxicity to human adenocarcinoma cell line SK-HEP-1 and normal cell lines of human lymphocytes and Chang liver cells. Thus, these compounds were exhibited the dose-dependent manner in HL-60 cells and the IC(50) were 10.8 and 12.5 microM, respectively. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at G(2)/M phase, and a concomitant increase of cell population at G(1) phase. The apoptosis induced by these two compounds was also demonstrated by DNA fragmentation assay and microscopic observation. These results suggest that the cytotoxic mechanism of both antitumor principle constituents might be the induction of apoptosis in HL-60 cells.

Estrutura de um fragmento de Floresta Atlântica em regeneração com ocorrência de Caesalpinia echinata Lam. (pau-brasil

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Liliane Baldan Zani

2012-09-01

Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n4p75   Este trabalho teve por objetivo analisar a estrutura fitossociológica de um fragmento remanescente de Floresta Atlântica em regeneração no município de Aracruz-ES em uma floresta de tabuleiro com ocorrência natural de Caesalpinia echinata Lam. Foram instaladas 10 unidades amostrais (parcelas de 10 x 50m sendo amostrados todos os indivíduos com DAP≥5cm e <10cm. Ao todo foram amostrados 500 indivíduos distribuídos em 181 espécies. As famílias com maior riqueza foram Leguminosae (35, Sapotaceae (18 e Myrtaceae (14. As espécies mais importantes foram Caesalpinia echinata Lam., Eugenia tinguyensis Cambess. e Pterocarpus rohrii Vahl. O índice de Shannon (H’ foi de 4,89 e a equabilidade (J’ foi de 0,94. Essa área é um dos últimos fragmentos remanescentes com pau-brasil nativo do estado do Espírito Santo e a população dessa espécie encontra-se bem preservada no local, ocorrendo com grande frequência, enfatizando a importância da preservação de pequenos fragmentos florestais para conservação da biodiversidade.

SUBSTRATOS E TEMPERATURAS PARA TESTE DE GERMINAÇÃO EM SEMENTES DE MYRTACEAE

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Juliano Pereira Gomes

2016-01-01

Full Text Available The Brazilian species of the Myrtaceae comprises several arborescent and shrubby plants that are used to produce fruit for fresh consumption or industrialization. Information about the seed quality evaluations for these species are scarce in the literature, mainly related to the adequacy of the germination tests. This study tests different substrates and temperatures to test the germination of Acca sellowiana (O. Berg Burret. (goiaba-serrana, Campomanesia xanthocarpa O. Berg (guabiroba, Eugenia involucrata DC. (cereja-do- mato and Eugenia pyriformis Camb. (uvaia seeds. Two seed lots were collected at different locations, one for each species, according to the requirements of the selection matrices. Sand and germitest paper towel rolls substrates were used, moistened with distilled water, and submitted to temperatures of 15 °C, 25 °C, 30 °C, 35 °C and 20-30 °C under constant light, on P.D.A. germinators. The experimental delineation was entirely randomized with four repetitions of 25 seeds/treatment/lot/specie, and treatment means were compared using the Tukey test (P < 0.01. The germination tests can be conducted at 25 °C using paper roll substrate for Eugenia pyriformis and E. involucrate . Both substrates can be used to Acca sellowiana germination test at 25 °C. The temperature of 25 °C and alternating 20-30 °C are indicated for Campomanesia xanthocarpa , as well as sand and paper roll substrates.

Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L.

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Fiuza, Tatiana S; Silva, Paulo C; De Paula, José R; Tresvenzol, Leonice M F; Sabóia-Morais, Simone M T

2009-01-01

This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an experimental model. The ethanol extract and fractions were administered to the fish orally with their feed. Twenty-four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that the crude extract and the ethyl, chloroform and hexane fractions induced vasodilation, vascular congestion and toxicity due to the presence of eosinophilic granular cells, rodlet cells, some leukocytic infiltrate and rare focal necroses. The Nile tilapia proved to be a satisfactory model for screening plant products.

Fenologia da floração e frutificação de mirtáceas nativas da floresta com araucária Phenology of blooming and fruiting of myrtaceae native species of araucaria forest

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Moeses Andrigo Danner

2010-03-01

Full Text Available Estudos de fenologia são importantes para definição de vários tratos culturais de espécies frutíferas. Assim, estudou-se a fenologia da floração e da frutificação de mirtáceas nativas da Floresta com Araucária, na região sudoeste do Paraná. Foram feitas observações fenológicas em pitangueira, cerejeira-do-mato, uvalheira, guabirobeira e araçazeiro, de julho de 2007 a fevereiro de 2008, em três plantas por espécie. Foram determinadas as curvas de crescimento dos frutos com base nos seus valores de massa da matéria seca, em duas avaliações semanais. A floração das espécies ocorreu entre o final de agosto e o início de novembro. A cerejeira-do-mato é a espécie mais precoce (ciclo de 43 dias, com maturação dos frutos entre meados e fim de outubro, enquanto o araçazeiro apresenta ciclo reprodutivo mais longo (98 dias, com maturação durante o mês de fevereiro. Há diferenças no comportamento do acúmulo de matéria seca nos frutos entre genótipos da mesma espécie.Studies of phenology are important for the development of various cultural treatments of fruit species. Therefore, it was studied the phenology of blooming and fruiting of Myrtaceae native species of Araucaria Forest, in the Southwest of Paraná state, Brazil. Observations about phenology of Eugenia uniflora, Eugenia involucrata, Eugenia pyriformis, Campomanesia xantocarpa, and Psidium cattleyanum were conducted from July 2007 to February 2008, in three plants of each species. There to manufacture the curves of the fruits growth based in mass of dry matter, in two weekly ratings. The flowering of species occurred between late August and early November. The Eugenia involucrata is the earliest (cycle of 43 days, with ripening between mid and end of October, while the Psidium cattleyanum presents reproductive cycle longer (98 days, with maturity during the month of February. There are differences in behavior accumulation of mass of dry matter between

Spectrophotometric Quantification of Flavonoids in Herbal Material, Crude Extract, and Fractions from Leaves of Eugenia uniflora Linn.

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Ramos, Rhayanne T M; Bezerra, Isabelle C F; Ferreira, Magda R A; Soares, Luiz Alberto Lira

2017-01-01

The traditional use of Eugenia uniflora L. ("Pitanga") is reported due to several properties, which have often been related to its flavonoid content. The aim was to evaluate analytical procedures for quantification of total flavonoids content (TFCs) by ultraviolet-visible (UV-Vis) spectrophotometry in the herbal material (HM), crude extract (CE), and fractions from leaves of E. uniflora . The method for quantification of flavonoids after complexation with aluminum chloride (AlCl 3 ) was evaluated: amount of sample (0.25-1.5 g); solvent (40%-80% ethanol); reaction time and AlCl 3 concentration (2.5%-7.5%). The procedures by direct dilution (DD) and after acid hydrolysis (AH) were used and validated for HM and CE and applied to the aqueous fraction (AqF), hexane fraction, and ethyl acetate fractions (EAF). The ideal conditions of analysis were ethanol 80% as solvent; 0.5 g of sample; λmax of 408 (DD) and 425 nm (AH); 25 min after addition of AlCl 3 5%. The procedures validated for standards and samples showed linearity ( R 2 > 0.99) with limit of detection and limit of quantification between 0.01 and 0.17 mg/mL (rutin and quercetin); and 0.03 and 0.09 mg/mL (quercetin), for DD and AH, respectively. The procedures were accurate (detect, practice, and repair 90%), and stable under robustness conditions (luminosity, storage, reagents, and equipment). The TFCs in AqF and EAF were 0.65 g% and 17.72 g%, calculated as rutin. UV-Vis methods for quantification of TFC in HM, CE, and fractions from leaves of E. uniflora were suitably validated. Regarding the analysis of fractions, the EAF achieved enrichment of about nine times in the content of flavonoids. The total flavonoids content (TFCs) of herbal material, crude extract, and fractions from Eugenia uniflora can be quantified by ultraviolet-visibleThe spectrophotometric methods (direct dilution and acid hydrolysis) were reproducible and able to quantify TFC in raw material and derivatives from leaves of E. uniflora Higher

Pitanga (Eugenia uniflora L.) fruit juice and two major constituents thereof exhibit anti-inflammatory properties in human gingival and oral gum epithelial cells.

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Josino Soares, Denise; Walker, Jessica; Pignitter, Marc; Walker, Joel Michael; Imboeck, Julia Maria; Ehrnhoefer-Ressler, Miriam Margit; Montenegro Brasil, Isabella; Somoza, Veronika

2014-11-01

Pitanga, Eugenia uniflora L., is a tropical fruit, which may be consumed as juice. While beneficial health effects of Eugenia uniflora L. leaf extracts have extensively been studied, limited data are available on an anti-inflammatory potential of pitanga juice. The aim of the presented study was to investigate anti-inflammatory properties of pitanga juice with regards to a prevention of inflammation-related periodontal diseases. For this purpose, six healthy volunteers swirled pitanga juice, containing 35% pitanga pulp, for 10 min. Thereafter, oral gum epithelial cells were harvested using a sterile brush and stimulated with lipopolysaccharides from Porphyromonas gingivalis (PG-LPS) for 6 h. Furthermore, human gingival fibroblasts (HGF-1) were used to elucidate the anti-inflammatory potential of pitanga juice constituents, cyanidin-3-glucoside and oxidoselina-1,3,7(11)-trien-8-one, in juice representative concentrations of 119 μg ml(-1) and 30 μg ml(-1), respectively. For the first time, an anti-inflammatory impact of pitanga juice on gingival epithelial cells was shown by means of an attenuation of IL-8 release by 55 ± 8.2% and 52 ± 11% in non-stimulated and PG-LPS-stimulated cells, respectively. In addition, both cyanidin-3-glucoside and oxidoselina-1,3,7(11)-trien-8-one reduced the LPS-stimulated CXCL8 mRNA expression by 50 ± 15% and 37 ± 18% and IL-8 release by 52 ± 9.9% and 45 ± 3.7% in HGF-1 cells, when concomitantly incubated with 10 μg ml(-1)PG-LPS for 6 h, revealing an anti-inflammatory potential of the volatile compound oxidoselina-1,3,7(11)-trien-8-one for the first time.

Eugenia e Fundação Rockefeller no Brasil: a saúde como proposta de regeneração nacional Eugenics and the Rockefeller Foundation in Brazil: health as an instrument of national regeneration

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Elisabete Kobayashi

2009-12-01

Full Text Available Nas primeiras décadas do século XX, a Fundação Rockefeller atuou de modo decisivo na implementação de ações voltadas para a saúde pública no Brasil, especialmente naquilo denominado de movimento sanitarista. Nesse mesmo período, a eugenia brasileira era equivalente ao sanitarismo, onde defendia-se a máxima de que "sanear era eugenizar". Vários trabalhos discutem o movimento sanitário, a questão da saúde pública no Brasil, a Fundação Rockefeller, a eugenia e seus ativistas. Entretanto, é escassa a discussão a respeito da Rockefeller e o movimento eugenista brasileiro. É necessário alertar que embora nos deparemos com questões ligadas à raça, teorias sobre genética ou evolução, este trabalho não tem como objetivo analisar tais assuntos com profundidade. O que buscamos construir foi o cenário no qual a eugenia de maneira geral, e a brasileira, especificamente, se desenrolou trazendo à tona as posturas de alguns eugenistas brasileiros.In the first decades of the twentieth century, the work of the Rockefeller Foundation was decisive for the implementation of public health initiatives in Brazil, especially in the so-called public health movement. At that time, Brazilian eugenics was the same as public health, as expressed in the maxim "to sanitize is to eugenize". There are several studies discussing the public health movement, the issue of public health in Brazil, the Rockefeller Foundation, eugenics and its supporters. However, there is little discussion on the relationship between the Rockefeller Foundation and the Brazilian eugenics movement. It is worth to mention that although there are issues related to race, and theories on genetics and evolution, this work does not intend to examine these issues in depth. The aim was to recreate the scenario in which eugenics in general, and particularly in the Brazilian case, was developed, exposing the positions of some of the Brazilian eugenicists.

Variation in the carotenoid composition of the lycopene-rich Brazilian fruit Eugenia uniflora L.

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Porcu, Ornella M; Rodriguez-Amaya, Delia B

2008-12-01

The indigenous pitanga (Eugenia uniflora L.) is now marketed and commercially processed in Brazil. In the present work, the carotenoids of the pitanga fruit from two states and at two stages of ripening, as well as of processed pitanga products (frozen pulp and juice, the brands being designated as A, B and C) commercialized in Campinas, São Paulo, were determined by HPLC. As compared to ripe pitanga from Medianeira, Paraná, those from Campinas had significantly higher (all-E)-lycopene (14.0 vs. 71.1 microg/g), (13Z)-lycopene (1.1 vs. 5.0 microg/g) and (all-E)-gamma-carotene (1.6 vs. 3.8 microg/g) levels. Significant increases in most of the carotenoids occurred from the partially ripe to the ripe fruits, with (all-E)-lycopene doubling its concentration in fruits from both states. Pitanga was found to be one of the richest fruit sources of carotenoids, particularly lycopene, but the processed products had much lower lycopene content. The mean (all-E)-lycopene concentration was 16.6 microg/g for frozen pulp brand A, 23.0 microg/g for bottled juice brand B and 25.6 microg/g for bottled juice brand C. Optimization of processing is therefore needed to guarantee better retention of this important carotenoid.

Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity.

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Oliveira, M D L; Andrade, C A S; Santos-Magalhães, N S; Coelho, L C B B; Teixeira, J A; Carneiro-da-Cunha, M G; Correia, M T S

2008-03-01

The aim of this work was to analyse the antimicrobial properties of a purified lectin from Eugenia uniflora L. seeds. The E. uniflora lectin (EuniSL) was isolated from the seed extract and purified by ion-exchange chromatography in DEAE-Sephadex with a purification factor of 11.68. The purified lectin showed a single band on denaturing electrophoresis, with a molecular mass of 67 kDa. EuniSL agglutinated rabbit and human erythrocytes with a higher specificity for rabbit erythrocytes. The haemagglutination was not inhibited by the tested carbohydrates but glycoproteins exerted a strong inhibitory action. The lectin proved to be thermo resistant with the highest stability at pH 6.5 and divalent ions did not affect its activity. EuniSL demonstrated a remarkable nonselective antibacterial activity. EuniSL strongly inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella sp. with a minimum inhibitory concentration (MIC) of 1.5 microg ml(-1), and moderately inhibited the growth of Bacillus subtilis, Streptococcus sp. and Escherichia coli with a MIC of 16.5 microg ml(-1). EuniSL was found to be effective against bacteria. The strong antibacterial activity of the studied lectin indicates a high potential for clinical microbiology and therapeutic applications.

Improving effects of the extracts from Eugenia uniflora on hyperglycemia and hypertriglyceridemia in mice.

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Arai, I; Amagaya, S; Komatsu, Y; Okada, M; Hayashi, T; Kasai, M; Arisawa, M; Momose, Y

1999-12-15

EtOH (70%) extracts from the leaves of Eugenia uniflora were separated into six fractions with different polarity and molecular size, i.e. NP-1-NP-6. In an oral glucose tolerance test, NP-1 and 4 inhibited the increase in plasma glucose level. However, in an intraperitoneal glucose tolerance test, such an inhibitory effect was not seen. Thus, the effects of NP-1 and 4 were apparently due to the inhibition of glucose absorption from the intestine. In a sucrose tolerance test, all fractions inhibited the increase in plasma glucose level. In an oral corn oil tolerance test, NP-3 and 4 showed an inhibitory effect on the increase in plasma triglycerides level. On the other hand, NP-3, 4, 5 and 6 inhibited maltase and sucrase activities and all fractions except for NP-1 showed an inhibitory effect on lipase activity dose-dependently. The inhibition of the increase in plasma glucose level by NP-3, 4, 5 and 6 in the oral sucrose tolerance test and the inhibition of the increase in plasma triglycerides by NP-3 and 4 in the oral corn oil tolerance test were apparently due to the inhibition of the decomposition of carbohydrates and fats in the intestine, respectively.

Synergisms in Alpha-glucosidase Inhibition and Antioxidant Activity of Camellia sinensis L. Kuntze and Eugenia uniflora L. Ethanolic Extracts

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Vinholes, Juliana; Vizzotto, Márcia

2017-01-01

Background: Camellia sinensis, the most consumed and popular beverages worldwide, and Eugenia uniflora, a Brazilian native species, have been already confirmed to have beneficial effects in the treatment of diabetes mellitus. However, their potential acting together against an enzyme linked to this pathology has never been exploited. Objective: The aim of this study was to evaluate the inhibitory properties of individual and combined ethanolic extracts of the leaves of C. sinensis and E. uniflora over alpha-glucosidase, a key digestive enzyme used on the Type 2 diabetes mellitus (T2DM) control. In addition, their inhibitory activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and peroxyl radicals was also assayed. Materials and Methods: Enzyme inhibition and antioxidant potential were assessed based on in vitro assays. Total phenolic compounds, carotenoids, and chlorophylls A and B were achieved using spectrophotometric methods. Results: E. uniflora was almost 40 times more active on alpha-glucosidase than C. sinensis and combined extracts showed a significant synergistic effect with an obtained IC50 value almost 5 times lower than the theoretical value. C. sinensis extract was twice more active than E. uniflora concerning DPPH•, in contrast, E. uniflora was almost 10 times more effective than C. sinensis on inhibition of peroxyl radicals with a significant synergistic effect for combined extracts. The extracts activities may be related with their phytochemicals, mainly phenolic compounds, and chlorophylls. Conclusion: Combined C. sinensis and E. uniflora ethanolic extracts showed synergistic effect against alpha-glucosidase and lipid peroxidation. These herbal combinations can be used to control postprandial hyperglycemia and can also provide antioxidant defenses to patients with T2DM. SUMMARY Alfa-glucosidase and antioxidant Interaction between Camellia sinensis L. Kuntze and Eugenia uniflora L. ethanolic extracts was investigated.Extracts showed

Synergisms in Alpha-glucosidase Inhibition and Antioxidant Activity of Camellia sinensis L. Kuntze and Eugenia uniflora L. Ethanolic Extracts.

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Vinholes, Juliana; Vizzotto, Márcia

2017-01-01

Camellia sinensis , the most consumed and popular beverages worldwide, and Eugenia uniflora , a Brazilian native species, have been already confirmed to have beneficial effects in the treatment of diabetes mellitus. However, their potential acting together against an enzyme linked to this pathology has never been exploited. The aim of this study was to evaluate the inhibitory properties of individual and combined ethanolic extracts of the leaves of C. sinensis and E. uniflora over alpha-glucosidase, a key digestive enzyme used on the Type 2 diabetes mellitus (T2DM) control. In addition, their inhibitory activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH • ) and peroxyl radicals was also assayed. Enzyme inhibition and antioxidant potential were assessed based on in vitro assays. Total phenolic compounds, carotenoids, and chlorophylls A and B were achieved using spectrophotometric methods. E. uniflora was almost 40 times more active on alpha-glucosidase than C. sinensis and combined extracts showed a significant synergistic effect with an obtained IC 50 value almost 5 times lower than the theoretical value. C. sinensis extract was twice more active than E. uniflora concerning DPPH • , in contrast, E. uniflora was almost 10 times more effective than C. sinensis on inhibition of peroxyl radicals with a significant synergistic effect for combined extracts. The extracts activities may be related with their phytochemicals, mainly phenolic compounds, and chlorophylls. Combined C. sinensis and E. uniflora ethanolic extracts showed synergistic effect against alpha-glucosidase and lipid peroxidation. These herbal combinations can be used to control postprandial hyperglycemia and can also provide antioxidant defenses to patients with T2DM. Alfa-glucosidase and antioxidant Interaction between Camellia sinensis L. Kuntze and Eugenia uniflora L. ethanolic extracts was investigated.Extracts showed synergistic effect over alpha-glucosidase and peroxyl radicals

Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae in the hepatopancreas of Oreochromis niloticus L

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TATIANA S FIUZA

2009-01-01

Full Text Available This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an experimental model. The ethanol extract and fractions were administered to the fish orally with their feed. Twenty-four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that the crude extract and the ethyl, chloroform and hexane fractions induced vasodilation, vascular congestion and toxicity due to the presence of eosinophilic granular cells, rodlet cells, some leukocytic infiltrate and rare focal necroses. The Nile tilapia proved to be a satisfactory model for screening plant products.

Antinociceptive and hypothermic evaluation of the leaf essential oil and isolated terpenoids from Eugenia uniflora L. (Brazilian Pitanga).

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Amorim, Ana Carolina L; Lima, Cleverton Kleiton F; Hovell, Ana Maria C; Miranda, Ana Luisa P; Rezende, Claudia M

2009-10-01

Eugenia uniflora L. (Myrtaceae), known as Brazilian cherry tree, is a fruity tree spread all over Brazil used in popular medicine to treat inflammations, rheumatic pain and fever, as hypoglycemic, diuretic and has been widely used in the cosmetics industry. The present study discusses the chemical composition, the antinociceptive and hypothermic profile of the essential oil of pitangueira leaves. The chemical composition was evaluated by GC-MS and the main constituent of the oil was characterized, after isolation, as a mixture of atractylone (1) and 3-furanoeudesmene (2). The essential oil, its pentane fraction and the isolated mixture of sesquiterpenes (1 and 2), given orally, significantly inhibited the acetic acid-induced abdominal constrictions, increased the latency time in hot plate test and showed a hypothermic effect. The results suggest that the responsible for the antinociceptive and hypothermic effect were the isolated furanosesquiterpenes. These findings provided additional pharmacological information and may contribute for the use of Brazilian cherry tree as a phytomedicine.

A new approach for quantifying furanodiene and curzerene: a case study on the essential oils of Eugenia uniflora L., Myrtaceae (pitangueira leaves

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Roberto Chang

2011-03-01

Full Text Available The essential oil obtained from the leaves of Eugenia uniflora L., Myrtaceae, which grows in the Brazilian savannah, was studied by gas chromatography mass spectrometry (GC-MS. Furanodiene (1.2% was thermally rearranged to curzerene (85.1% to produce a combined content of 86.3%. GC analysis carried out under mild conditions (with a constant temperature of 100 ºC showed that the furanodiene concentration was three-fold greater than the curzerene concentration, i.e., the essential oil contained 64.7% furanodiene and 21.6% curzerene. Germacrene B also rearranged to γ-elemene and the concentration of both was 2.3%. Special care should be taken when conventional gas chromatography analysis is used for quantifying compounds that can rearrange at high temperatures.

Eficiência de extratos vegetais no controle de Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae, em laboratório

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Junir Antonio Lutinski

2010-04-01

Full Text Available http://dx.doi.org/10.5007/2175-7925.2010v23n1p83 Vulgarmente conhecida como “vaquinha”, Diabrotica speciosa (Germar, 1824 ocorre na maioria dos estados brasileiros, destacando-se como uma das mais importantes pragas do feijoeiro e do milho. Visando avaliar a atividade inseticida de extratos brutos aquosos de nove espécies vegetais sobre os adultos deste inseto, realizou-se este trabalho. O experimento, conduzido em laboratório, foi realizado sob delineamento completamente casualizado, com dez tratamentos e quatro repetições. Para tal, utilizou-se um frasco, contendo em seu interior cinco espécimes adultos e uma folha de feijoeiro (Phaseolus vulgaris Linnaeus previamente imersa no extrato, tampado com um recorte de pano poroso e fixado por uma borracha. A variável avaliada foi número de espécimes de D. speciosa vivos. Os tratamentos consistiram em extratos de salvia (Salvia officinalis Linnaeus, cravo (Eugenia caryophyllata Thunb, noz-moscada (Myristica fragans Houtt, cinamomo (Melia azedarach Linnaeus, timbó (Ateleia glazioveana Baill, eucalipto (Eucalyptus citriodora Hook, canela (Cinnamomum zeylanicum Blume, figueira (Ficus microcarpa Linnaeus f., alecrim (Rosmarinus officinalis Linnaeus e a testemunha (apenas água destilada. As avaliações de sobrevivência foram realizadas a cada 24 horas, durante 10 dias. Sobre o número de espécimes vivos efetuou-se a análise de variância em bifatorial (10 extratos x 11 horários após a aplicação. As médias foram agrupadas pelo teste de Duncan ao nível de 5% de probabilidade de erro. Os extratos mais eficientes foram o timbó, noz-moscada e cinamomo, com porcentagens de eficiência variando entre 80,4% e 100%.

Photosynthetic metabolism and quality of Eugenia pyriformis Cambess. seedlings on substrate function and water levels.

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Scalon, Silvana P Q; Jeromini, Tatiane S; Mussury, Rosilda M; Dresch, Daiane M

2014-12-01

The aim of this research was to evaluate the quality and photosynthetic metabolism of "uvaia" seedlings (Eugenia pyriformis Cambess.) on different substrates and water regimes. The seeds were sown in tubes of 50 x 190 mm in the following substrates: Sand (S), Latosol + Sand (L + S) (1:1), Latosol + Sand + Semi Decomposed Poultry Litter (L + S1 + PL) ( 1:1:0.5), Latosol + Sand + Semi Decomposed Poultry Litter (L + S2 + PL) (1:2:0.5), Latosol + Bioplant® (L + B) (1:1), and the water levels assessed were 50, 75 and 100% of water retention capacity. At 60, 90, 120 and 150 days the seedlings were evaluated according to their chlorophyll index, leaf area (cm2) and Dickson Quality Index (DQI) and at 150 days their internal concentration of carbon (mol m-2 s-1), stomatal conductance (mol m-2 s-1), transpiration rate (mmol m-2 s-1), photosynthesis (µmol m-2 s-1) and efficiency of water use (µmol de CO2 / mmol de H2O). Until their 150th days, the seedlings had higher quality and photosynthetic metabolism when cultured with substrates containing latosol + sand + poultry litter on the two variations assessed and water retention capacity of 50%.

Photosynthetic metabolism and quality of Eugenia pyriformis Cambess. seedlings on substrate function and water levels

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SILVANA P.Q. SCALON

2014-12-01

Full Text Available The aim of this research was to evaluate the quality and photosynthetic metabolism of “uvaia” seedlings (Eugenia pyriformis Cambess. on different substrates and water regimes. The seeds were sown in tubes of 50 x 190 mm in the following substrates: Sand (S, Latosol + Sand (L + S (1:1, Latosol + Sand + Semi Decomposed Poultry Litter (L + S1 + PL ( 1:1:0.5, Latosol + Sand + Semi Decomposed Poultry Litter (L + S2 + PL (1:2:0.5, Latosol + Bioplant® (L + B (1:1, and the water levels assessed were 50, 75 and 100% of water retention capacity. At 60, 90, 120 and 150 days the seedlings were evaluated according to their chlorophyll index, leaf area (cm2 and Dickson Quality Index (DQI and at 150 days their internal concentration of carbon (mol m–2 s–1, stomatal conductance (mol m–2 s–1, transpiration rate (mmol m–2 s–1, photosynthesis (µmol m–2 s–1 and efficiency of water use (µmol de CO2 / mmol de H2O. Until their 150th days, the seedlings had higher quality and photosynthetic metabolism when cultured with substrates containing latosol + sand + poultry litter on the two variations assessed and water retention capacity of 50%.

Efficacy of combination herbal product (Curcuma longa and Eugenia jambolana) used for diabetes mellitus.

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Rao, Sadia Saleem; Najam, Rahila

2016-01-01

The purpose of this research was to evaluate the efficacy of a combination herbal product that is traditionally used for managing diabetes mellitus. Herbal drug contains Curcuma longa and Eugenia jambolanain the ratio of 1:1. It was orally administered at the dose of 1082 mg/70 kg twice a day for a period of 6 weeks to alloxan induced diabetic rats and compared with glibenclamide (standard). The effects of drug were observed at intervals, with respect to random and fasting glucose levels. HbA1C was also monitored after the drug treatment to monitor the overall diabetic effect. Results revealed that the combination of two herbs significantly reduced fasting and random glucose levels with HbA1C of less than 6% (p<0.001) in comparison to diabetic control. The control of fasting blood glucose levels by herbal combination is similar to the standard drug, glibenclamide (p<0.05). Random glucose levels by herbal combination is better than standard drug after one week and six weeks of treatment (p<0.01 and p<0.001 respectively) and similar after third week of treatment (p<0.05). Also, herbal drug combination showed HbA1C closer to the standard drug. It shows that this herbal combination can be of potential benefit in managing diabetes mellitus in future.

Racismo de estado e tanatopolítica: Sobre o paradoxo do nazismo em Michel Foucault e Giorgio Agamben

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Francisco Bruno Pereira Diógenes

2013-12-01

Full Text Available Este artigo propõe-se a expor as reflexões de dois filósofos contemporâneos, quais sejam, Michel Foucault e Giorgio Agamben, de modo a promover um paralelo,ou mesmo uma analogia, entre duas noções que permeiam as obras dos respectivos autores. Estas noções consistem no que Foucault chamou de Racismo de Estado, e no que Agamben nomeou de Tanatopolítica. O contexto, e tambémo objeto, no qual se efetiva este intento é, em uma palavra, a política totalitária do Estado nazista.Ambos os autores compreendem os fenômenos totalitários a partir do ponto de vista da biopolítica, entretanto, ao tratar do Estado Nacional-socialista, Foucault identifica nele um paradoxo, sem, contudo desenvolver com maior profundidade a imbricação que ali se deu. Agamben, por sua vez, concebe semelhante paradoxo como pertencendo a uma forma de fazer política que já traz, desde a sua origem, os fundamentos que permitiram a emergência do fenômeno da forma como surgiu e que fora identificada pelos ditos autores, ou seja, trazendo em si tanto elementos antigos, como a glória e os ideais de sangue, quanto modernos, como a eugenia. A pesquisa bibliográfica que aqui se desenvolveu deu-se na leitura de, basicamente, três obras dos supraditos autores, quais sejam, Em defesa da sociedade, História da sexualidade I – A vontade de saber, de um lado (Foucault, e Homo sacer – O poder soberano e a vida nua I, de outro (Agamben.

Did it really happen? Memory, history and myth in Eugenia Tsoulis´ Between the ceiling and the sky

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Catalina Ribas Segura

2009-06-01

Full Text Available World War II, the Nazi occupation and several dictatorships forced many Greek men and women into migration. In 1952 Greece signed an agreement on assisted migration to Australia and more than “250 000 Greek and Cypriot migrants from Greece (1952-74, Rumania (1952-8, Egypt and the Middle East (1952-2 [sic], Cyprus (1974-84 and other politically turbulent countries of Eastern Europe and Latin America” moved to Australia (Tamis, Anastasios M. The Greeks in Australia, 2005: 47. The lives of those migrants changed radically as they left home behind. Some of them, or their children, wrote fictional texts explaining some of their experiences. An example of this is Eugenia Tsoulis´ Behind the Ceiling and the Sky (1998, where the main characters live their lives between present and past and between memories and myths, on the one hand, and facts and the lifeworld that surround them, on the other. This paper will analyse this novel and the sometimes blurred boundaries between memory, history and myth.

Hypoglycemic and hypolipidemic effects of triterpenoid-enriched Jamun (Eugenia jambolana Lam.) fruit extract in streptozotocin-induced type 1 diabetic mice.

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Xu, Jialin; Liu, Tingting; Li, Yuanyuan; Yuan, Chunhui; Ma, Hang; Seeram, Navindra P; Liu, Feifei; Mu, Yu; Huang, Xueshi; Li, Liya

2018-06-20

The edible berries of Eugenia jambolana Lam. (known as Jamun) are consumed in various parts of the world. Our previous studies revealed that a triterpenoid-enriched Jamun fruit extract (TJFE) showed beneficial effects on glucose homeostasis in non-diabetic mice. Herein, the anti-diabetic effects of TJFE (100 mg kg-1 by oral gavage for ten days) were evaluated in streptozotocin (STZ)-induced type 1 diabetic mice. TJFE significantly attenuated STZ-induced hyperglycemia and glucose intolerance, suppressed the abnormal elevation of hepatic gluconeogenesis, and improved dyslipidemia in the mice. Histopathology and mechanism-based studies revealed that TJFE preserved the architecture and function of pancreatic islets, attenuated insulin secretion deficiency, enhanced insulin/Akt signaling transduction, reduced lipogenic gene expression, and prevented the abnormal activation of Erk MAPK in the liver tissues of the STZ-induced diabetic mice. The current study adds to previously published data supporting the potential beneficial effects of this edible fruit on diabetes management.

Comparison of the interfacial properties of Eugenia uniflora and Triticum vulgaris lectins.

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Andrade, Cesar A S; Oliveira, Maria D L; Santos-Magalhães, Nereide S; Correia, Maria T S; de Melo, Celso P

2009-01-01

We have investigated the interfacial and dielectric properties of EuniSL, a recently purified lectin obtained from seeds of Eugenia uniflora (EuniSL), through surface pressure (Pi) and surface potential (DeltaV) measurements of its floating monolayers at the 2.0

Quorum Quenching and Microbial Control through Phenolic Extract of Eugenia Uniflora Fruits.

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Rodrigues, Adeline Conceição; Zola, Flávia Guimarães; Ávila Oliveira, Brígida D'; Sacramento, Nayara Thais Barbosa; da Silva, Elis Regina; Bertoldi, Michele Corrêa; Taylor, Jason Guy; Pinto, Uelinton Manoel

2016-10-01

We describe the characterization of the centesimal composition, mineral and phenolic content of Eugenia uniflora fruit and the determination of the antioxidant, antimicrobial and quorum quenching activities of the pulp phenolic extract. Centesimal composition was determined according to standard methods; trace elements were measured by total reflection X-ray fluorescence spectroscopy. The phenolic compounds were extracted by solid-phase chromatography and quantified by spectrophotometry. Antioxidant activity was determined by using 3 different methods. Antimicrobial activity was evaluated against a panel of foodborne microorganisms and antiquorum sensing activity in Chromobacterium violaceum was performed by measuring inhibition of quorum sensing dependent violacein production. The centesimal composition (per 100 g of pulp) was as follows: protein 3.68 ± 0.21 g, lipids 0.02 ± 0.03 g, carbohydrates 10.31 g and fiber 2.06 g. Trace elements (mg/g of pulp) were determined as: K 0.90, Ca 3.36, Fe 0.60, Zn 0.17, Cl 0.56, Cr 0.06, Ni 0.04, and Cu 0.07. The pulp is a source of phenolic compounds and presents antioxidant activity similar to other berries. The fruit phenolic extract inhibited all tested bacteria. We also found that the fruit phenolic extract at low subinhibitory concentrations inhibited up to 96% of violacein production in C. violaceum, likely due to the fruit's phenolic content. This study shows the contribution of E. uniflora phenolic compounds to the antioxidant, antimicrobial and the newly discovered quorum quenching activity, all of which could be used by the food and pharmaceutical industries to develop new functional products. © 2016 Institute of Food Technologists®.

Chemical and biological evaluation of essential oils from two species of Myrtaceae - Eugenia uniflora L. and Plinia trunciflora (O. Berg) Kausel.

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Lago, João Henrique G; Souza, Elisângela Dutra; Mariane, Bruna; Pascon, Renata; Vallim, Marcelo A; Martins, Roberto Carlos C; Baroli, Adriana A; Carvalho, Bianca A; Soares, Marisi G; dos Santos, Roberta T; Sartorelli, Patricia

2011-11-25

The chemical composition and antimicrobial activity of essential oils obtained from leaves of two Myrtaceae species-Eugenia uniflora L. and Plinia trunciflora (O. Berg) Kausel-were determined. Analysis by GC/MS as well as determination of Kovatz indexes indicated atractylone (26.78%) and curzerene (17.96%) as major constituents of E. uniflora oil and α-cadinol (19.15%), apiole (11.15%) and cubenol (5.43%) as main components in P. trunciflora oil. Both essential oils were tested for antimicrobial activity against yeasts and bacteria. E. uniflora and P. trunciflora essential oils were active towards two Gram-positive bacteria, Streptococcus equi and Staphylococcus epidermis. In addition, biological activity of both essential oils was detected for pathogenic yeasts of the genus Candida and Cryptococcus. E. uniflora was active towards all yeast tested and exhibited interesting minimal inhibitory concentrations (0.11 to 3.75 mg/mL) across a broad spectrum of activity.

Myrtaceae da restinga no norte do Espírito Santo, Brasil

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Augusto Giaretta

2015-01-01

Full Text Available Myrtaceae está entre as famílias mais ricas em espécies nas restingas do Espírito Santo. Este estudo objetivou fazer o inventário e a caracterização das espécies de Myrtaceae ocorrentes na vegetação de restinga no norte do Espírito Santo. Foram registradas 52 espécies distribuídas em 10 gêneros. O gênero mais representado foi Eugenia (19 espécies, seguido de Myrcia (12, Marlierea (6, Psidium (4, Myrciaria (3, Calyptranthes (2, Campomanesia (2, Neomitranthes (2, Blepharocalyx (1 e Plinia (1. Foram registradas duas  espécies endêmicas (Eugenia inversa e Myrcia limae. As formações vegetais  com maior número de espécies foram a florestal não inundável (40 espécies,  seguida da arbustiva fechada não inundável (19 e florestal inundável (19. São apresentadas chaves para identificação das espécies, descrições, comentários,  distribuição geográfica e ilustrações dos caracteres diagnósticos.

Photosynthesis and oxidative stress in the restinga plant species Eugenia uniflora L. exposed to simulated acid rain and iron ore dust deposition: Potential use in environmental risk assessment

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Rust Neves, Natalia; Oliva, Marco Antonio; Cruz Centeno, Danilo da; Costa, Alan Carlos; Ferreira Ribas, Rogerio [Departamento de Biologia Vegetal, Universidade Federal de Vicosa, Av. PH Rolfs, Campus, Vicosa, Minas Gerais, 36570-000 (Brazil); Gusmao Pereira, Eduardo, E-mail: egpereira@gmail.com [Departamento de Biologia Vegetal, Universidade Federal de Vicosa, Av. PH Rolfs, Campus, Vicosa, Minas Gerais, 36570-000 (Brazil)

2009-06-01

The Brazilian sandy coastal plain named restinga is frequently subjected to particulate and gaseous emissions from iron ore factories. These gases may come into contact with atmospheric moisture and produce acid rain. The effects of the acid rain on vegetation, combined with iron excess in the soil, can lead to the disappearance of sensitive species and decrease restinga biodiversity. The effects of iron ore dust deposition and simulated acid rain on photosynthesis and on antioxidant enzymes were investigated in Eugenia uniflora, a representative shrub species of the restinga. This study aimed to determine the possible utility of this species in environmental risk assessment. After the application of iron ore dust as iron solid particulate matter (SPM{sub Fe}) and simulated acid rain (pH 3.1), the 18-month old plants displayed brown spots and necrosis, typical symptoms of iron toxicity and injuries caused by acid rain, respectively. The acidity of the rain intensified leaf iron accumulation, which reached phytotoxic levels, mainly in plants exposed to iron ore dust. These plants showed the lowest values for net photosynthesis, stomatal conductance, transpiration, chlorophyll a content and electron transport rate through photosystem II (PSII). Catalase and superoxide dismutase activities were decreased by simulated acid rain. Peroxidase activity and membrane injury increased following exposure to acid rain and simultaneous SPM{sub Fe} application. Eugenia uniflora exhibited impaired photosynthetic and antioxidative metabolism in response to combined iron and acid rain stresses. This species could become a valuable tool in environmental risk assessment in restinga areas near iron ore pelletizing factories. Non-invasive evaluations of visual injuries, photosynthesis and chlorophyll a fluorescence, as well as invasive biochemical analysis could be used as markers.

Photosynthesis and oxidative stress in the restinga plant species Eugenia uniflora L. exposed to simulated acid rain and iron ore dust deposition: Potential use in environmental risk assessment

International Nuclear Information System (INIS)

Rust Neves, Natalia; Oliva, Marco Antonio; Cruz Centeno, Danilo da; Costa, Alan Carlos; Ferreira Ribas, Rogerio; Gusmao Pereira, Eduardo

2009-01-01

The Brazilian sandy coastal plain named restinga is frequently subjected to particulate and gaseous emissions from iron ore factories. These gases may come into contact with atmospheric moisture and produce acid rain. The effects of the acid rain on vegetation, combined with iron excess in the soil, can lead to the disappearance of sensitive species and decrease restinga biodiversity. The effects of iron ore dust deposition and simulated acid rain on photosynthesis and on antioxidant enzymes were investigated in Eugenia uniflora, a representative shrub species of the restinga. This study aimed to determine the possible utility of this species in environmental risk assessment. After the application of iron ore dust as iron solid particulate matter (SPM Fe ) and simulated acid rain (pH 3.1), the 18-month old plants displayed brown spots and necrosis, typical symptoms of iron toxicity and injuries caused by acid rain, respectively. The acidity of the rain intensified leaf iron accumulation, which reached phytotoxic levels, mainly in plants exposed to iron ore dust. These plants showed the lowest values for net photosynthesis, stomatal conductance, transpiration, chlorophyll a content and electron transport rate through photosystem II (PSII). Catalase and superoxide dismutase activities were decreased by simulated acid rain. Peroxidase activity and membrane injury increased following exposure to acid rain and simultaneous SPM Fe application. Eugenia uniflora exhibited impaired photosynthetic and antioxidative metabolism in response to combined iron and acid rain stresses. This species could become a valuable tool in environmental risk assessment in restinga areas near iron ore pelletizing factories. Non-invasive evaluations of visual injuries, photosynthesis and chlorophyll a fluorescence, as well as invasive biochemical analysis could be used as markers.

Efeito de extratos de plantas com atividade inseticida no controle de Microtheca ochroloma Stal (Col.: Chrysomelidae, em laboratório

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Sônia Thereza Bastos Dequech

2008-01-01

Full Text Available http://dx.doi.org/10.5007/2175-7925.2008v21n1p41 Extratos de plantas com atividade inseticida foram testados no controle de  Microtheca ochroloma (Col.: Chrysomelidae, uma importante praga de brassicáceas nas fases larval e adulta. Duas larvas com três dias de idade, mantidas sob condições de laboratório (T:25ºC, U.R:70% e 14 horas de fotofase, foram colocadas em tubos de vidro contendo folha de  couve-chinesa (Brassica chinensis previamente tratada com extratos aquosos (10% p/v de folha de cinamomo (Melia azedarach, de ramo de  cinamomo e de pó-de-fumo (Nicotiana tabacum. O mesmo procedimento foi repetido em dois ensaios com insetos adultos. No primeiro, foram utilizados todos os extratos anteriormente citados mais o extrato de Dal- Neem (produto comercial à base de Azadirachta indica. No segundo, os insetos foram expostos a extratos de frutos de pimenta malagueta (Capsicum frutescens e de folhas de pitangueira (Eugenia uniflora, de jambolão (Syzygium cuminii e de eucalipto (Eucalyptus sp.. Todos os testes constaram de 10 insetos por tratamento, com cinco repetições no primeiro ensaio com insetos adultos e com seis repetições nos demais. As  observações foram realizadas diariamente até o quinto dia, objetivando avaliar a mortalidade dos insetos. Todos os extratos testados  resultaram num controle efetivo de larvas de M.ochroloma.Em  relação aos insetos adultos, apenas os extratos de pó-de-fumo e de Dal- Neem apresentaram efi ciência de controle.

A regenerative route for Eugenia uniflora L. (Myrtaceae through in vitro germination and micropropagation

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Paulo Roberto Diniz da Silva

2014-07-01

Full Text Available Eugenia uniflora is a tree species native from Central and South America, largely employed in the popular medicine, in the cosmetics and pharmaceutical industries and also consumed in natura. Aiming to provide plant material with high sanity and genetic uniformity for the establishment of commercial plantations, we developed a protocol for seeds disinfestation, in vitro germination and in vitro propagation of this species through organogenesis. Fruits of E. uniflora were obtained from wild trees growing in the Pampa biome, Southern Brazil. Seeds were disinfested using ethanol 70% (10 min and NaOCl 1.25% (10 or 25 min. Shoot apexes and nodal segments of non-contaminated plantlets were cultivated in verification medium AS30 during 20 days, posteriorly in ½MS medium supplemented with sucrose, IBA and BAP during 45 days and acclimatized in greenhouse. Disinfesting seeds with ethanol 70% (10 min and NaOCl 1.25% (25 min allowed germination with significantly lower contamination (2.0% and production of healthy explants for the micropropagation. No difference concerning size and contamination was observed for the propagation using shoot apexes or nodal segments as explant. Acclimatized plants revealed normal phenotype and healthy appearance. This regenerative route can be applied for mass clonal propagation from seeds of cross-pollinated or self-pollinated selected trees aiming the establishment of commercial plantations of E. uniflora and other Myrtaceae species.

Persistência de óleos essenciais em milho armazenado, submetido à infestação de gorgulho do milho Persistence of essential oils in stored maize submitted to infestation of maize weevil

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Rodrigo Leandro Braga de Castro Coitinho

2010-07-01

Full Text Available Os óleos essenciais e os compostos constituintes têm sido pesquisados quanto a sua atividade inseticida contra pragas de grãos armazenados. Neste trabalho, avaliou-se a persistência de óleos essenciais em milho armazenado, submetido à infestação do gorgulho do milho, Sitophilus zeamais Mots. (Coleoptera: Curculionidae. A persistência dos óleos e do eugenol foi avaliada no período inicial (logo após a impregnação e aos 30, 60, 90 e 120 dias de armazenamento. As mortalidades de S. zeamais, no período inicial, variaram entre 93,8 (Piper hispidinervum, Melaleuca leucadendron e eugenol e 100% (Eugenia uniflora, frutos verdes de Schinus terebinthifolius e Piper marginatum. A partir dos 30 dias, as mortalidades, de modo geral, decresceram, com exceção de P. marginatum (92,2%, que alcançou 53,1% de mortalidade aos 120 dias de armazenamento. De acordo com as equações de regressão ajustadas para o número de S. zeamais emergidos em todo o período de armazenamento, apenas não houve significância para os óleos de S. terebinthifolius, P. marginatum e testemunha. Em relação à média geral, o óleo de P. marginatum foi o mais persistente, proporcionando emergência de apenas 0,30 insetos, diferindo dos óleos restantes, do eugenol e da testemunha. Os demais tratamentos só diferiram em relação à testemunha.The essential oils and constituent compounds have been studied for their insecticidal activity against stored grain pests. In this research, persistence of the essential oils in stored maize subject to infestation by maize weevil, Sitophilus zeamais Mots. (Coleoptera: Curculionidae were evaluated. Persistence of oils and eugenol were evaluated in the initial period (after impregnation and at 30, 60, 90 and 120 days of storage. Mortalities of S. zeamais in the initial period ranged between 93.8 (Piper hispidinervum, Melaleuca leucadendron and eugenol to 100% (Eugenia uniflora, green fruits of Schinus terebinthifolius and Piper

Evaluation of <em>HER2em> Gene Amplification in Breast Cancer Using Nuclei Microarray <em>in em>S>itu em>Hybridization

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Xuefeng Zhang

2012-05-01

Full Text Available Fluorescence<em> em>>in situ em>hybridization (FISH assay is considered the “gold standard” in evaluating <em>HER2/neu (HER2em> gene status. However, FISH detection is costly and time consuming. Thus, we established nuclei microarray with extracted intact nuclei from paraffin embedded breast cancer tissues for FISH detection. The nuclei microarray FISH (NMFISH technology serves as a useful platform for analyzing <em>HER2em> gene/chromosome 17 centromere ratio. We examined <em>HER2em> gene status in 152 cases of invasive ductal carcinomas of the breast that were resected surgically with FISH and NMFISH. <em>HER2em> gene amplification status was classified according to the guidelines of the American Society of Clinical Oncology and College of American Pathologists (ASCO/CAP. Comparison of the cut-off values for <em>HER2em>/chromosome 17 centromere copy number ratio obtained by NMFISH and FISH showed that there was almost perfect agreement between the two methods (κ coefficient 0.920. The results of the two methods were almost consistent for the evaluation of <em>HER2em> gene counts. The present study proved that NMFISH is comparable with FISH for evaluating <em>HER2em> gene status. The use of nuclei microarray technology is highly efficient, time and reagent conserving and inexpensive.

Neonatal Phosphate Nutrition Alters <em>in em>Vivo> and <em>in em>Vitro> Satellite Cell Activity in Pigs