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Sample records for ermeli jalad proovile

  1. ERM Ideas and Innovations: Digital Repository Management as ERM

    Science.gov (United States)

    Pinkas, María M.; Lin, Na

    2014-01-01

    This article describes the application of electronic resources management (ERM) to digital repository management at the Health Sciences and Human Services Library at the University of Maryland, Baltimore. The authors discuss electronic resources management techniques, through the application of "Techniques for Electronic Management,"…

  2. ERM immersion vaccination and adjuvants

    DEFF Research Database (Denmark)

    Skov, J.; Chettri, J. K.; Jaafar, R. M.

    2015-01-01

    Two candidate adjuvants were tested with a commercial ERM dip vaccine (AquaVac™ Relera, MSD Animal Health) for rainbow trout in an experimental design compatible with common vaccination practices at farm level, i.e. immersion of fish in vaccine (±adjuvant) for 30 s. The adjuvants were...... the commercial product Montanide™ IMS 1312 VG PR (SEPPIC), and a soluble and ≥98% pure β-glucan from yeast (Saccharomyces cerevisiae) (Sigma-Aldrich). Hence, five experimental groups in duplicate were established and exposed to vaccine and adjuvants in the following combinations: AquaVac™ Relera (alone); Aqua......Vac™ Relera + Montanide™; AquaVac™ Relera + β-glucan; Montanide™ (alone); and β-glucan (alone). Approximately 450 degree days post-vaccination, the fish were bath-challenged with live Yersinia ruckeri to produce survival curves. Blood, skin and gills were sampled at selected time points during the course...

  3. Negative in vitro selection identifies the rRNA recognition motif for ErmE methyltransferase

    DEFF Research Database (Denmark)

    Nielsen, A K; Douthwaite, S; Vester, B

    1999-01-01

    Erm methyltransferases modify bacterial 23S ribosomal RNA at adenosine 2058 (A2058, Escherichia coli numbering) conferring resistance to macrolide, lincosamide, and streptogramin B (MLS) antibiotics. The motif that is recognized by Erm methyltransferases is contained within helix 73 of 23S r...

  4. Erythromycin resistance caused by erm(A) subclass erm(TR) in a Danish invasive pneumococcal isolate: Are erm(A) pneumococcal isolates overlooked?

    DEFF Research Database (Denmark)

    Lambertsen, L.; Ekelund, K.; Hansen, D.S.

    2008-01-01

    Erm(A) is rarely reported as erythromycin resistance determinant in pneumococci. One invasive erm(A) isolate was initially tested intermediate erythromycin resistant using E-test. However, upon re-testing it was resistant, thus close to the breakpoint value. This may be a reason why erm(A) only r...

  5. ERMes: Open Source Simplicity for Your E-Resource Management

    Science.gov (United States)

    Doering, William; Chilton, Galadriel

    2009-01-01

    ERMes, the latest version of electronic resource management system (ERM), is a relational database; content in different tables connects to, and works with, content in other tables. ERMes requires Access 2007 (Windows) or Access 2008 (Mac) to operate as the database utilizes functionality not available in previous versions of Microsoft Access. The…

  6. Value-driven ERM: making ERM an engine for simultaneous value creation and value protection.

    Science.gov (United States)

    Celona, John; Driver, Jeffrey; Hall, Edward

    2011-01-01

    Enterprise risk management (ERM) began as an effort to integrate the historically disparate silos of risk management in organizations. More recently, as recognition has grown of the need to cover the upside risks in value creation (financial and otherwise), organizations and practitioners have been searching for the means to do this. Existing tools such as heat maps and risk registers are not adequate for this task. Instead, a conceptually new value-driven framework is needed to realize the promise of enterprise-wide coverage of all risks, for both value protection and value creation. The methodology of decision analysis provides the means of capturing systemic, correlated, and value-creation risks on the same basis as value protection risks and has been integrated into the value-driven approach to ERM described in this article. Stanford Hospital and Clinics Risk Consulting and Strategic Decisions Group have been working to apply this value-driven ERM at Stanford University Medical Center. © 2011 American Society for Healthcare Risk Management of the American Hospital Association.

  7. Domain V of 23S rRNA contains all the structural elements necessary for recognition by the ErmE methyltransferase

    DEFF Research Database (Denmark)

    Vester, B; Douthwaite, S

    1994-01-01

    to erythromycin and clindamycin. The degree of resistance corresponds to the level of ermE expression. In turn, ermE expression also correlates with the proportion of 23S rRNA molecules that are dimethylated at adenine 2058. The methyltransferase was isolated in an active, concentrated form from E. coli...... disrupted by removal of magnesium ions. We conclude that the main features that are specifically recognized by the ErmE methyltransferase are displayed within the primary and secondary structures of 23S rRNA domain V....

  8. ERM booster vaccination of Rainbow trout using diluted bacterin

    DEFF Research Database (Denmark)

    Schmidt, Jacob Günther; Henriksen, Niels H.; Buchmann, Kurt

    2016-01-01

    Enteric Red Mouth Disease ERM caused by Yersinia ruckeri infection is associated with morbidity and mortality in salmonid farming but immersion vaccination of fry may confer some protection for a number of months. Revaccination of rainbow trout, even by use of diluted ERM immersion vaccine, can......:10) in April 2015 was followed 3 months later (July 2015) by 1 h bathing of rainbow trout in bacterin (diluted 1:650 or 1:1700) in order to evaluate if this time saving vaccination methodology can improve immunity and protection. Trout were subjected in farms to natural Y. ruckeri exposure in June and July...

  9. e-Learning system ERM for medical radiation physics education.

    Science.gov (United States)

    Stoeva, Magdalena; Cvetkov, Asen

    2005-09-01

    The objective of this paper is to present the Education for Radiation in Medicine (ERM) e-Learning System. The system was developed, tested and piloted in the Inter-University Medical Physics Centre, Plovdiv, Bulgaria. It was based on the results of EU Project TEMPUS S-JEP 09826. The ERM e-Learning System is an integrated on-line system for remote education covering aspects of Medical Radiation Physics education (M.Sc. level). It provides user-friendly interface and optimised functionality with three different access levels: trainee, professor and administrator. The minimum server requirements and the standard client side working environment turn the system into a good, cost effective and easy to support solution for remote education.

  10. Entrprise Risk Management : Das COSO-ERM-Framework

    OpenAIRE

    Ruud, Flemming; Sommer, Katerina

    2006-01-01

    Das Enterprise Risk Management (ERM) ist ein wichtiges Element der Corporate Governance. Es kann Unternehmen bei der Zielerreichung unterstützen, indem es hilft, Chancen und Risiken zu erkennen und geeignete Massnahmen zu ergreifen. Die Weiterentwicklung des Internal-Control-Framework zu einem Enterprise-Risk-Management-Framework, dessen Grund sätze, Ziele und Komponenten, dessen Nutzen und Grenzen sowie die Unterschiede zum ursprünglichen COSO-ICFramework ist Gegenstand dieses Artikels.

  11. Enterprise Risk Management : Das COSO-ERM-Framework

    OpenAIRE

    Ruud, Flemming; Sommer, Katerina

    2006-01-01

    Das Enterprise Risk Management (ERM) ist ein wichtiges Element der Corporate Governance. Es kann Unternehmen bei der Zielerreichung unterstützen, indem es hilft, Chancen und Risiken zu erkennen und geeignete Massnahmen zu ergreifen. Die Weiterentwicklung des Internal-Control-Framework zu einem Enterprise-Risk-Management-Framework, dessen Grund sätze, Ziele und Komponenten, dessen Nutzen und Grenzen sowie die Unterschiede zum ursprünglichen COSO-ICFramework ist Gegenstand dieser Ausführunge...

  12. Redundant control of migration and adhesion by ERM proteins in vascular smooth muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Baeyens, Nicolas; Latrache, Iman; Yerna, Xavier [Laboratory of Cell Physiology, IoNS, Université Catholique de Louvain (Belgium); Noppe, Gauthier; Horman, Sandrine [Pôle de Recherche Cardiovasculaire, IREC, Université Catholique de Louvain (Belgium); Morel, Nicole, E-mail: nicole.morel@uclouvain.be [Laboratory of Cell Physiology, IoNS, Université Catholique de Louvain (Belgium)

    2013-11-22

    Highlights: •The three ERM proteins are expressed in vascular smooth muscle cell. •ERM depletion inhibited PDGF-evoked migration redundantly. •ERM depletion increased cell adhesion redundantly. •ERM depletion did not affect PDGF-evoked Ca signal, Rac1 activation, proliferation. •ERM proteins control PDGF-induced migration by regulating adhesion. -- Abstract: Ezrin, radixin, and moesin possess a very similar structure with a C-terminal actin-binding domain and a N-terminal FERM interacting domain. They are known to be involved in cytoskeleton organization in several cell types but their function in vascular smooth muscle cells (VSMC) is still unknown. The aim of this study was to investigate the role of ERM proteins in cell migration induced by PDGF, a growth factor involved in pathophysiological processes like angiogenesis or atherosclerosis. We used primary cultured VSMC obtained from rat aorta, which express the three ERM proteins. Simultaneous depletion of the three ERM proteins with specific siRNAs abolished the effects of PDGF on cell architecture and migration and markedly increased cell adhesion and focal adhesion size, while these parameters were only slightly affected by depletion of ezrin, radixin or moesin alone. Rac1 activation, cell proliferation, and Ca{sup 2+} signal in response to PDGF were unaffected by ERM depletion. These results indicate that ERM proteins exert a redundant control on PDGF-induced VSMC migration by regulating focal adhesion turn-over and cell adhesion to substrate.

  13. COSO ERM y la gestión de riesgos

    OpenAIRE

    Sánchez Sánchez, Liliana Ruth; Facultad de Ciencias Contables, Universidad Nacional Mayor de San Marcos

    2016-01-01

    Considerando que cada entidad enfrenta una grancantidad de riesgos que afectan las diferentes partesde la organización; la gerencia no solamente necesitamanejar los riesgos individuales; sino también, comprenderlos impactos interrelacionados. Para ello, lametodología COSO ERM fue diseñada para identificareventos potenciales que afectasen a una entidad,evaluar y responder a los riesgos detectados, para queestén dentro de los límites de nivel aceptables comoparte de una buena administración.La ...

  14. eRME - Rapid Maxillary Expansion in the economic way

    Directory of Open Access Journals (Sweden)

    Sonali Mahadevia

    2011-01-01

    Full Text Available Aim and Objectives: Rapid Maxillary Expansion constitutes a routine clinical procedure in orthodontics, involving separation of mid-palatine suture which is usually done with help of the Hyrax screw. However, because of its high cost, the use has been limited, especially in institutions. So, the purpose of this study was to construct an economical device which can expand the maxillary arch in growing patients. Materials and Methods: Six patients having constricted maxilla and posterior skeletal crossbite were randomly selected from the Department of Orthodontics. A unique, easy and simple alternative device for expanding the maxillary arch called economic Rapid Maxillary Expander (eRME has been fabricated at about one-tenth the cost of the conventional Hyrax. Pre- and post-treatment effects were statistically tested by using paired t-test at 0.05 level of significance. Results and Conclusion: The study results showed an average expansion in canine, premolar and molar regions of 4.4 mm, 6.8 mm and 9.4 mm, respectively, having significant difference pre-and post-treatment. Thus, it shows that maxillary expansion is efficiently possible with the application of this newly constructed device named eRME. This appliance also acts as a fixed retainer to avoid relapse, hence negating the need for a separate retainer.

  15. Improved ERM vaccination efficacy using combined vaccine administration methods

    DEFF Research Database (Denmark)

    Buchmann, Kurt; Desmukh, Sidhartha; Chettri, Jiwan Kumar

    2012-01-01

    We have previously shown that immersion vaccination (30 sec) using the Aquavac Relera vaccine (containing formalin killed Yersinia ruckeri serotype O1 of both biotypes 1 and 2) provides the best protection (when compared to other commercial ERM vaccines on the Danish market) against infection...... following i. p. challenge using Y. ruckeri O1, biotype 2, which at present is the main bacterial pathogen in fingerling trout production in Denmark. Despite a significant protection conferred by this vaccine (immersion) some mortality could be observed following challenge. We have therefore performed...... a study in order to elucidate if different vaccine administration methods (using Aquavac Relera) can improve protection and reduce mortality of exposed trout following challenge with this particular pathogen. Rainbow trout (mean weight 7.8 g) reared at the Bornholm Salmon Hatchery under pathogen free...

  16. Learning about Fundamentals: The Widening of the French ERM Bands in 1993,

    NARCIS (Netherlands)

    Garretsen, Harry; Knot, Klaas; Nijsse, Erwin

    Learning about Fundamentals: The Widening of the French ERM Bands in 1993. — The authors incorporate a Bayesian learning model into a fairly general model of exchange rate determination in discrete time. The model is applied to the period following the widening of the French-German ERM bands in

  17. Could Enterprise Risk Management (ERM Create, Protect and Enhance Shareholders’ Wealth Among Malaysian Listed Issuers

    Directory of Open Access Journals (Sweden)

    Bin Kasim Mohd Ariff

    2017-01-01

    Full Text Available The primary objective of this study is to explore the extent to which ERM implementation is capable of enhancing shareholders’ wealth. A sample of 283 companies operating in the Main Market of Bursa Malaysia was selected. This study utilized the definition of ERM as the independent variable and shareholders’ wealth as dependent variable. The overall observation revealed that ERM was significant in explaining the variation in shareholders’ wealth. Objective setting, event identification, risk response, control activities, information and communication, and monitoring substantiated the hypothesized relationship. The research framework of this study, which utilized assumptions from stewardship and agency theory, could serve as guidance for future research on organizational control and governance. The study fills the gap in literature which mostly concentrated on the USA and western countries. The CRO and the board should focus more advising functions at the earlier stage of the ERM implementation and monitoring functions once ERM matured.

  18. ErmF and ereD are responsible for erythromycin resistance in Riemerella anatipestifer.

    Science.gov (United States)

    Xing, Linlin; Yu, Hui; Qi, Jingjing; Jiang, Pan; Sun, Bingqing; Cui, Junsheng; Ou, Changcan; Chang, Weishan; Hu, Qinghai

    2015-01-01

    To investigate the genetic basis of erythromycin resistance in Riemerella anatipestifer, the MIC to erythromycin of 79 R. anatipestifer isolates from China and one typed strain, ATCC11845, were evaluated. The results showed that 43 of 80 (53.8%) of the tested R. anatipestifer strains showed resistance to erythromycin, and 30 of 43 erythromycin-resistant R. anatipestifer strains carried ermF or ermFU with an MIC in the range of 32-2048 μg/ml, while the other 13 strains carrying the ereD gene exhibited an MIC of 4-16 μg/ml. Of 30 ermF + R. anatipestifer strains, 27 (90.0%) carried the ermFU gene which may have been derived from the CTnDOT-like element, while three other strains carried ermF from transposon Tn4351. Moreover, sequence analysis revealed that ermF, ermFU, and ereD were located within the multiresistance region of the R. anatipestifer genome.

  19. The monetary framework after accession a political economy analysis of ERM2

    Directory of Open Access Journals (Sweden)

    Attila Fölsz

    2003-03-01

    Full Text Available The paper discusses the framework of ERM2 (the exchange rate mechanism of EMU, the Economic and Monetary Union and its suitability for accession countries / advanced transition economies. It analyzes the exchange rate mechanism from two aspects. First, it considers how ERM2 regulates the monetary cohabitation of "ins" (Eurozone members and "outs"(non-Eurozone members of EMU. Second it reviews how ERM2 is conducive to future euro-zone membership of the accession countries. From the first point of view it discusses whether ERM2 sufficiently protects "outs" from speculative attacks, and "ins" from competitive devaluation of the "outs". From the second angle it examines whether ERM2 provides an appropriate environment for "outs" to fulfill the convergence criteria, and whether in this case "ins" open the door for the "outs" to enter the euro-zone. The principal argument of the paper is that the more ERM2 fulfils its first task, that is to regulate the relationship of "ins" and "outs" in a satisfactory way, the less it fulfils its second task, that is to lead "outs" in the Eurozone. Apart from that, the present framework of ERM2 and the convergence criteria together are not suitable for a fast and quick entry of the Central and Eastern European accession countries to the euro area. In order to overcome these pitfalls the author makes some proposals as well.

  20. The monetary framework after accession – a political economy analysis of ERM2

    Directory of Open Access Journals (Sweden)

    Attila Fölsz

    2003-03-01

    Full Text Available The paper discusses the framework of ERM2 (the exchange rate mechanism of EMU, the Economic and Monetary Union and its suitability for accession countries / advanced transition economies. It analyzes the exchange rate mechanism from two aspects. First, it considers how ERM2 regulates the monetary cohabitation of "ins" (Eurozone members and "outs"(non-Eurozone members of EMU. Second it reviews how ERM2 is conducive to future euro-zone membership of the accession countries. From the first point of view it discusses whether ERM2 sufficiently protects "outs" from speculative attacks, and "ins" from competitive devaluation of the "outs". From the second angle it examines whether ERM2 provides an appropriate environment for "outs" to fulfill the convergence criteria, and whether in this case "ins" open the door for the "outs" to enter the euro-zone. The principal argument of the paper is that the more ERM2 fulfils its first task, that is to regulate the relationship of "ins" and "outs" in a satisfactory way, the less it fulfils its second task, that is to lead "outs" in the Eurozone. Apart from that, the present framework of ERM2 and the convergence criteria together are not suitable for a fast and quick entry of the Central and Eastern European accession countries to the euro area. In order to overcome these pitfalls the author makes some proposals as well.

  1. Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates

    Directory of Open Access Journals (Sweden)

    Vivian de Lima Spode Coutinho

    Full Text Available INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B, inducible resistance (iMLS B, and resistance only to macrolides and streptogramins B (MS B. The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by the disk diffusion method. Isolates with iMLS B phenotype were tested by double-disk induction method. All isolates were tested by a genotypic assay, PCR with specific primers. RESULTS: A total of 46.7% of staphylococci were positive for cMLS B; 3.3% for iMLS B and 3.3% for MS B. One or more erm genes were present in 50.1% of isolates. The gene ermA was detected in 49 isolates, ermC in 29 and ermB in 3. CONCLUSION: The prevalence of the ermA, ermB and ermC genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes.

  2. A novel Erm monomethyltransferase in antibiotic-resistant isolates of Mannheimia haemolytica and Pasteurella multocida

    DEFF Research Database (Denmark)

    Desmolaize, Benoit; Rose, Simon; Warrass, Ralf

    2011-01-01

    Mannheimia haemolytica and Pasteurella multocida are aetiological agents commonly associated with respiratory tract infections in cattle. Recent isolates of these pathogens have been shown to be resistant to macrolides and other ribosome-targeting antibiotics. Direct analysis of the 23S r......RNAs by mass spectrometry revealed that nucleotide A2058 is monomethylated, consistent with a Type I erm phenotype conferring macrolide-lincosamide resistance. The erm resistance determinant was identified by full genome sequencing of isolates. The sequence of this resistance determinant, now termed erm(42...

  3. Learning Bounds of ERM Principle for Sequences of Time-Dependent Samples

    Directory of Open Access Journals (Sweden)

    Mingchen Yao

    2015-01-01

    Full Text Available Many generalization results in learning theory are established under the assumption that samples are independent and identically distributed (i.i.d.. However, numerous learning tasks in practical applications involve the time-dependent data. In this paper, we propose a theoretical framework to analyze the generalization performance of the empirical risk minimization (ERM principle for sequences of time-dependent samples (TDS. In particular, we first present the generalization bound of ERM principle for TDS. By introducing some auxiliary quantities, we also give a further analysis of the generalization properties and the asymptotical behaviors of ERM principle for TDS.

  4. CRITIQUES TOWARDS COSO’S ENTERPRISE RISK MANAGEMENT (ERM) FRAMEWORK IN ITS BASIC ASSUMPTIONS

    OpenAIRE

    Kurniawanti, Ika Atma

    2010-01-01

    Most professionals in internal control, risk management and other similar bailiwickshave agreed that Enterprise Risk Management discourses would’ve invariablyreferred to what the COSO had produced recently: the framework underlying ERM.But this paper takes a bit different stance that views several problematic issuesstem from unclear conceptions of either the basic premise underlying ERM or thenature of some ERM’s components outlined by COSO. This paper notes that, atleast, there are three poi...

  5. From Millennium ERM to Proquest 360 Resource Manager: Implementing a new Electronic Resources Management System ERMS in an International Graduate Research University in Saudi Arabia

    KAUST Repository

    Ramli, Rindra M.

    2017-05-17

    An overview of the Recommendation Study and the subsequent Implementation of a new Electronic Resources Management system ERMS in an international graduate research university in the Kingdom of Saudi Arabia. It covers the timeline, deliverables and challenges as well as lessons learnt by the Project Team.

  6. PEMETAAN RISIKO DI INDUSTRI PENYAMAKAN KULIT DENGAN PENDEKATAN ENTERPRISE RISK MANAGEMENT (ERM

    Directory of Open Access Journals (Sweden)

    Helen Wiryani

    2014-09-01

    Full Text Available ABSTRACTThe aim of this study were 1 identify the risks faced by PT XYZ, 2 measure and map the risk of PT XYZ, 3 formulating an effective risk mitigation strategy for PT XYZ. The method of risk management with enterprise risk management approach (ERM was applied. Risk management with ERM can help companies better prepared to cope with uncertainty, increase the added value and competitive advantage. ERM approach to risk mapping is done by referring to the eight components of ERM. The first three components, namely internal environment, objective setting, and event identification, were used to observe PT.  XYZ situation and identify the risks that exist. A review of the business processes in PT XYZ shows that there are various strategic, operating, reporting, and compliance risks identified in the stages of the business process. Results showed 1 the risks found in the PT XYZ stages of business process were related to the field of strategic, operating, reporting, and compliance, 2 the amount of risk experienced by PT XYZ were reflected in the risk map formed on the measurement of risk based on probability and impact, 3 strategies need to be developed for effective risk mitigation for PT XYZ were prioritized addressing the highest risk first then to a lower risk.Keywords: ERM, FGD, risk management, upholstery industry, risk mappingABSTRAKTujuan  penelitian  ini  adalah 1 mengidentifikasi risiko yang dihadapi PT XYZ, 2 mengukur dan memetakan risiko PT XYZ, 3 merumuskan strategi mitigasi risiko yang efektif bagi PT XYZ. Metode pengelolaan risiko dengan pendekatan enterprise risk management (ERM. Pengelolaan risiko dengan ERM dapat membantu perusahaan lebih siap dalam  mengatasi ketidakpastian,  meningkatkan nilai tambah, dan keunggulan bersaing. Pemetaan risiko dengan pendekatan ERM dilakukan dengan berpedoman pada delapan komponen ERM. Tiga komponen pertama yaitu internal environment, objective setting, dan event identification dilakukan untuk

  7. SLK-dependent activation of ERMs controls LGN–NuMA localization and spindle orientation

    Science.gov (United States)

    Machicoane, Mickael; de Frutos, Cristina A.; Fink, Jenny; Rocancourt, Murielle; Lombardi, Yannis; Garel, Sonia; Piel, Matthieu

    2014-01-01

    Mitotic spindle orientation relies on a complex dialog between the spindle microtubules and the cell cortex, in which F-actin has been recently implicated. Here, we report that the membrane–actin linkers ezrin/radixin/moesin (ERMs) are strongly and directly activated by the Ste20-like kinase at mitotic entry in mammalian cells. Using microfabricated adhesive substrates to control the axis of cell division, we found that the activation of ERMs plays a key role in guiding the orientation of the mitotic spindle. Accordingly, impairing ERM activation in apical progenitors of the mouse embryonic neocortex severely disturbed spindle orientation in vivo. At the molecular level, ERM activation promotes the polarized association at the mitotic cortex of leucine-glycine-asparagine repeat protein (LGN) and nuclear mitotic apparatus (NuMA) protein, two essential factors for spindle orientation. We propose that activated ERMs, together with Gαi, are critical for the correct localization of LGN–NuMA force generator complexes and hence for proper spindle orientation. PMID:24958772

  8. Molecular characterization of Streptococcus agalactiae isolates harboring small erm(T)-carrying plasmids.

    Science.gov (United States)

    Compain, Fabrice; Hays, Constantin; Touak, Gérald; Dmytruk, Nicolas; Trieu-Cuot, Patrick; Joubrel, Caroline; Poyart, Claire

    2014-11-01

    Among 1,827 group B Streptococcus (GBS) strains collected between 2006 and 2013 by the French National Reference Center for Streptococci, 490 (26.8%) strains were erythromycin resistant. The erm(T) resistance gene was found in six strains belonging to capsular polysaccharides Ia, III, and V and was carried by the same mobilizable plasmid, which could be efficiently transferred by mobilization to GBS and Enterococcus faecalis recipients, thus promoting a broad dissemination of erm(T). Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  9. Interference of an ERM-vaccine with a VHS-DNA vaccine in rainbow trout

    DEFF Research Database (Denmark)

    Lorenzen, Ellen; Einer-Jensen, Katja; Rasmussen, Jesper Skou

    results showed that intraperitoneal injection of a commercial vaccine against Enteric Redmouth Disease (ERM) based on formalin-killed bacteria in oil adjuvant immediately followed by intramuscular injection of an experimental DNA-vaccine against VHSV, decreased the protective effect of the DNA...... are vaccinated with a VHS-DNA vaccine dose according to their size, a simultaneous intraperitoneal vaccination against ERM can compromise the protective effect of the DNA-vaccine. The negative effect appears to be strongest in the early phase following vaccination. The immune mechanisms behind this interference...

  10. Koolera paneb Haiti valmiduse proovile / Hendrik Vosman

    Index Scriptorium Estoniae

    Vosman, Hendrik

    2010-01-01

    Haitis puhkenud koolerapuhang on nõudnud juba 253 inimelu, nakatunute arv ületab 3100 piiri, bakter võib sealsete ebahügieeniliste olude tõttu kaasa tuua teise humanitaarkatastroofi pärast 12. jaanuari maavärinat. Kaart

  11. Presence of erm gene classes in Gram-positive bacteria of animal and human origin in Denmark

    DEFF Research Database (Denmark)

    Jensen, Lars Bogø; Frimodt-Moller, N.; Aarestrup, Frank Møller

    1999-01-01

    and 68 staphylococcal erythromycin resistant isolates of animal and human origin. At least one of these genes was detected in 88% of the isolates. Four isolates contained more than one ei il? gene. ermB dominated among the enterococci (88%) and streptococci (90%) and ermC among staphylococci (75...

  12. Uudised : Indi-sahinad. Anne Erm ja Eero Raun on heliloojad. Imelaste lemmik on heavy metal

    Index Scriptorium Estoniae

    2007-01-01

    Ameerika laulja Kimya Dawson 7. juulil Kilingi-Nõmmel minifestivalil "Schilling". Pianist Martti Raide esitab festivali Eesti muusika päevad 2007 suurkontserdil 16. aprillil Eero Rauna klaveripala "Tritonata", üllatusheliloojaks on ka "Jazzkaare" produtsent Anne Erm. Inglismaal Noorte Talentide Rahvuslikus Akadeemias tehtud uuringust

  13. Validation of an instrument to measure older adults' expectations regarding movement (ERM.

    Directory of Open Access Journals (Sweden)

    Nabila Dahodwala

    Full Text Available BACKGROUND: Many individuals with Parkinson's disease are not diagnosed and treated. Attitudes about aging and related help-seeking may affect the timely diagnosis of Parkinson's disease. Our objectives were to develop measures of older adults' expectations regarding movement with aging, specifically related to parkinsonism, and their beliefs about seeking healthcare for the diagnosis and treatment of parkinsonism. METHODS: We established content and face validity from interviews with experts, review of the literature, and pre-testing with key informants. Two 9-item instruments resulted: Expectations Regarding Movement (ERM and Healthcare Seeking Beliefs for parkinsonism (HSB. These instruments were administered to 210 older adults at senior centers to investigate internal consistency and construct validity. RESULTS: 192 (91% of the older adults completed more than 90% of the survey. The mean age was 76; 17 (9% reported parkinsonism. Both scales demonstrated good internal consistency (α = 0.90. Factor analysis supported construct validity of the ERM and HSB scores. Older age, lower education, worse self-reported health and African American race each were associated with lower ERM scores, but not HSB scores. CONCLUSION: The ERM, a brief measure of expectations regarding movement with aging, shows reliability and validity. This scale may be useful in identifying older adults at increased risk for under-identification of Parkinson's disease. Further work is needed to measure healthcare seeking for parkinsonism.

  14. Indication of transposition of a mobile DNA element containing the vat(D) and erm(B) genes in Enterococcus faecium

    DEFF Research Database (Denmark)

    Hammerum, Anette Marie; Flannagan, S.E.; Clewell, D.B.

    2001-01-01

    The vat(D) and erm(B) genes encoding streptogramin resistance in Enterococcus faecium transferred together, and a direct physical link between erm(B) and vat(D) was detected. Both the vat(D) and erm(B) probes hybridized to fragments of different sizes in the donor and transconjugants, which...

  15. Oral and anal vaccination confers full protection against enteric redmouth disease (ERM) in rainbow trout

    DEFF Research Database (Denmark)

    Villumsen, Kasper Rømer; Neumann, Lukas; Otani, Maki

    2014-01-01

    The effect of oral vaccines against bacterial fish diseases has been a topic for debate for decades. Recently both M-like cells and dendritic cells have been discovered in the intestine of rainbow trout. It is therefore likely that antigens reaching the intestine can be taken up and thereby induce...... immunity in orally vaccinated fish. The objective of this project was to investigate whether oral and anal vaccination of rainbow trout induces protection against an experimental waterborne infection with the pathogenic enterobacteria Yersinia ruckeri O1 biotype 1 the causative agent of enteric redmouth...... disease (ERM). Rainbow trout were orally vaccinated with AquaVac ERM Oral (MERCK Animal Health) or an experimental vaccine bacterin of Y. ruckeri O1. Both vaccines were tested with and without a booster vaccination four months post the primary vaccination. Furthermore, two groups of positive controls were...

  16. Conserved SMP domains of the ERMES complex bind phospholipids and mediate tether assembly.

    Science.gov (United States)

    AhYoung, Andrew P; Jiang, Jiansen; Zhang, Jiang; Khoi Dang, Xuan; Loo, Joseph A; Zhou, Z Hong; Egea, Pascal F

    2015-06-23

    Membrane contact sites (MCS) between organelles are proposed as nexuses for the exchange of lipids, small molecules, and other signals crucial to cellular function and homeostasis. Various protein complexes, such as the endoplasmic reticulum-mitochondrial encounter structure (ERMES), function as dynamic molecular tethers between organelles. Here, we report the reconstitution and characterization of subcomplexes formed by the cytoplasm-exposed synaptotagmin-like mitochondrial lipid-binding protein (SMP) domains present in three of the five ERMES subunits--the soluble protein Mdm12, the endoplasmic reticulum (ER)-resident membrane protein Mmm1, and the mitochondrial membrane protein Mdm34. SMP domains are conserved lipid-binding domains found exclusively in proteins at MCS. We show that the SMP domains of Mdm12 and Mmm1 associate into a tight heterotetramer with equimolecular stoichiometry. Our 17-Å-resolution EM structure of the complex reveals an elongated crescent-shaped particle in which two Mdm12 subunits occupy symmetric but distal positions at the opposite ends of a central ER-anchored Mmm1 homodimer. Rigid body fitting of homology models of these SMP domains in the density maps reveals a distinctive extended tubular structure likely traversed by a hydrophobic tunnel. Furthermore, these two SMP domains bind phospholipids and display a strong preference for phosphatidylcholines, a class of phospholipids whose exchange between the ER and mitochondria is essential. Last, we show that the three SMP-containing ERMES subunits form a ternary complex in which Mdm12 bridges Mmm1 to Mdm34. Our findings highlight roles for SMP domains in ERMES assembly and phospholipid binding and suggest a structure-based mechanism for the facilitated transport of phospholipids between organelles.

  17. Macrolide resistance gene erm(TR) and erm(TR)-carrying genetic elements in Streptococcus agalactiae: characterization of ICESagTR7, a new composite element containing IMESp2907.

    Science.gov (United States)

    Mingoia, Marina; Morici, Eleonora; Marini, Emanuela; Brenciani, Andrea; Giovanetti, Eleonora; Varaldo, Pietro E

    2016-03-01

    The objective of this study was to investigate macrolide-resistant Streptococcus agalactiae isolates harbouring erm(TR), an erm(A) gene subclass, with emphasis on their erm(TR)-carrying genetic elements. Four erm(TR)-carrying elements have been described to date: three closely related (ICE10750-RD.2, Tn1806 and ICESp1108) in Streptococcus pyogenes, Streptococcus pneumoniae and S. pyogenes, respectively; and one completely different (IMESp2907, embedded in ICESp2906 to form ICESp2905) in S. pyogenes. Seventeen macrolide-resistant erm(TR)-positive S. agalactiae isolates were phenotypically and genotypically characterized. Their erm(TR)-carrying elements were explored by analysing the distinctive recombination genes of known erm(TR)-carrying integrative and conjugative elements (ICEs) and by PCR mapping. The new genetic context and organization of IMESp2907 in S. agalactiae were explored using several experimental procedures and in silico analyses. Five isolates harboured ICE10750-RD.2/Tn1806, five isolates harboured ICESp1108 and five isolates bore unknown erm(TR)-carrying elements. The remaining two isolates, exhibiting identical serotypes and pulsotypes, harboured IMESp2907 in a new genetic environment, which was further investigated in one of the two isolates, SagTR7. IMESp2907 was circularizable in S. agalactiae, as described in S. pyogenes. The new IMESp2907 junctions were identified based on its site-specific integration; the att sites were almost identical to those in S. pyogenes. In strain SagTR7, erm(TR)-carrying IMESp2907 was embedded in an erm(TR)-less internal element related to ICE10750-RD.2/Tn1806, which, in turn, was embedded in an ICESde3396-like element. The resulting whole ICE, ICESagTR7 (∼129 kb), was integrated into the chromosome downstream of the rplL gene, and was excisable in circular form and transferable by conjugation. This is the first study exploring erm(TR)-carrying genetic elements in S. agalactiae. © The Author 2015. Published by

  18. Crystal structure of Mdm12 reveals the architecture and dynamic organization of the ERMES complex.

    Science.gov (United States)

    Jeong, Hanbin; Park, Jumi; Lee, Changwook

    2016-12-01

    The endoplasmic reticulum-mitochondria encounter structure (ERMES) is a protein complex that plays a tethering role in physically connecting ER and mitochondria membranes. The ERMES complex is composed of Mdm12, Mmm1, and Mdm34, which have a SMP domain in common, and Mdm10. Here, we report the crystal structure of S. cerevisiae Mdm12. The Mdm12 forms a dimeric SMP structure through domain swapping of the β1-strand comprising residues 1-7. Biochemical experiments reveal a phospholipid-binding site located along a hydrophobic channel of the Mdm12 structure and that Mdm12 might have a binding preference for glycerophospholipids harboring a positively charged head group. Strikingly, both full-length Mdm12 and Mdm12 truncated to exclude the disordered region (residues 74-114) display the same organization in the asymmetric unit, although they crystallize as a tetramer and hexamer, respectively. Taken together, these studies provide a novel understanding of the overall organization of SMP domains in the ERMES complex, indicating that Mdm12 interacts with Mdm34 through head-to-head contact, and with Mmm1 through tail-to-tail contact of SMP domains. © 2016 The Authors.

  19. erm gene distribution among Norwegian Bacteroides isolates and evaluation of phenotypic tests to detect inducible clindamycin resistance in Bacteroides species.

    Science.gov (United States)

    Johnsen, Bjørn Odd; Handal, Nina; Meisal, Roger; Bjørnholt, Jørgen Vildershøj; Gaustad, Peter; Leegaard, Truls Michael

    2017-10-01

    The aims of this study were to describe the distribution of the most common erm genes in a collection of Norwegian Bacteroides isolates and to investigate whether the phenotypic tests for determining inducible clindamycin resistance among Bacteroides species recommended by EUCAST, NordicAST and the manufacturer of E-test(®), are effective. We investigated 175 unique Bacteroides isolates for the presence of erm(B), erm(F) and erm(G) genes, determined their minimum inhibitory concentrations (MICs) to clindamycin and categorised their susceptibility according to EUCAST breakpoints. 27 isolates were resistant to clindamycin. Furthermore, we investigated whether these recommended methods could detect inducible resistance in the Bacteroides isolates: 1) EUCAST recommendation: Dissociated resistance to erythromycin (clindamycin susceptible with erythromycin MIC > 32 mg/L), 2) NordicAST recommendation: Double disk diffusion test (DDD) or 3) Manufacturer of E-test(®)'s recommendation: prolonged incubation of clindamycin E-test(®) for 48 h. erm genes were detected in 30 (17%, 95% CI 12%-23%) of 175 Bacteroides isolates with erm(F) as the dominating gene. There were six (4%, 95% CI 1%-7%) of 148 clindamycin susceptible isolates harbouring erm genes, they were considered inducibly resistant to clindamycin. None of the methods for phenotypic detection of inducible clindamycin resistance performed satisfactory with sensitivities of 33%, 17% and 0% and specificities of 90%, 99% and 97% for dissociated resistance, DDD and prolonged incubation of clindamycin E-test(®), respectively. In our view, the scientific basis for investigating every Bacteroides isolate for inducible resistance to clindamycin is weak. Molecular detection of erm genes may prove a better option than the phenotypic methods we evaluated. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Dissemination of erm(B) and its associated multidrug-resistance genomic islands in Campylobacter from 2013 to 2015.

    Science.gov (United States)

    Liu, Dejun; Deng, Fengru; Gao, Yanan; Yao, Hong; Shen, Zhangqi; Wu, Congming; Wang, Yang; Shen, Jianzhong

    2017-05-01

    A total of 1372 Campylobacter isolates (1107 Campylobacter coli and 265 Campylobacter jejuni) were obtained from 3462 samples collected from slaughterhouses and farms in three representative regions of China (Shandong, Guangdong, and Shanghai) over three successive years (2013-2015). Of these, 84 (84/1372, 6.1%) were erm(B)-positive, and all 84 positive isolates were identified as C. coli (83 chicken isolates and one swine isolate). The prevalence of erm(B) in Campylobacter isolates was compared amongst the different regions and between the three years investigated. The rates of erm(B)-positive Campylobacter in Guangdong increased remarkably over the experimental period (3.8% to 22.8%), while their higher rates observed in Shanghai (4.4%) and Shandong (2.4%) occurred in 2015 and 2014. Further, 72 erm(B)-positive isolates were associated with the type V and VI multidrug-resistance genomic islands (MDRGIs), which have previously only been identified in human Campylobacter isolates, while one isolate of chicken origin contained the type II MDRGI, which has previously been detected in swine isolates. Expansion of the erm(B) in Campylobacter with similar PFGE and MLST type from chicken isolates from Shanghai and Guangdong to human isolates identified previously in Shanghai was also observed. The findings in this study confirmed previously observed trend of dissemination of erm(B) and MDRGIs in zoonotic Campylobacter isolates and provide new insights into the prevalence of erm(B)-positive Campylobacter isolates in chickens and swine from three representative regions of China over a consecutive 3-year period. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Novel Structure of Enterococcus faecium-Originated ermB-Positive Tn1546-Like Element in Staphylococcus aureus.

    Science.gov (United States)

    Wan, Tsai-Wen; Hung, Wei-Chun; Tsai, Jui-Chang; Lin, Yu-Tzu; Lee, Hao; Hsueh, Po-Ren; Lee, Tai-Fen; Teng, Lee-Jene

    2016-10-01

    We determined the resistance determinants in 274 erythromycin-resistant methicillin-susceptible Staphylococcus aureus (MSSA) isolates during a 13-year period, 2000 to 2012. The resistance phenotypes, inducible macrolide-lincosamide-streptogramin (iMLS), constitutive MLS (cMLS), and macrolide-streptogramin (MS) resistance phenotypes, were examined by a double-disk diffusion D test. The ermB gene was more frequent (35%; 97/274) than ermC (27%; 75/274) or ermA (21%; 58/274). All 97 ermB-positive isolates harbored Tn551 and IS1216V The majority (89/97) of ermB-positive isolates displayed the cMLS phenotype and carried mobile element structure (MES)-like structures, which has been previously reported in sequence type 59 (ST59) methicillin-resistant S. aureus (MRSA). The remaining 8 ermB-carrying isolates, belonging to ST7 (n = 4), ST5 (n = 3), and ST59 (n = 1), were sasK intact and did not carry MES-like structures. Unlike a MES-like structure that was located on the chromosome, the ermB elements on sasK-intact isolates were located on plasmids by S1 nuclease pulsed-field gel electrophoresis (PFGE) analysis and conjugation tests. Sequence data for the ermB-containing region (14,566 bp) from ST59 NTUH_3874 revealed that the best match was a Tn1546-like element in plasmid pMCCL2 DNA (GenBank accession number AP009486) of Macrococcus caseolyticus Tn1546 is recognized as an enterococcal transposon and was known from the vancomycin resistance gene cluster in vancomycin-resistant Enterococcus (VRE). So far, acquisitions of Tn1546 in S. aureus have occurred in clonal complex 5 (CC5) MRSA, but not in MSSA. This is the first report that MSSA harbors an Enterococcus faecium-originated ermB-positive Tn1546-like element located on a plasmid. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  2. Structure-function insights into direct lipid transfer between membranes by Mmm1-Mdm12 of ERMES.

    Science.gov (United States)

    Kawano, Shin; Tamura, Yasushi; Kojima, Rieko; Bala, Siqin; Asai, Eri; Michel, Agnès H; Kornmann, Benoît; Riezman, Isabelle; Riezman, Howard; Sakae, Yoshitake; Okamoto, Yuko; Endo, Toshiya

    2017-12-26

    The endoplasmic reticulum (ER)-mitochondrial encounter structure (ERMES) physically links the membranes of the ER and mitochondria in yeast. Although the ER and mitochondria cooperate to synthesize glycerophospholipids, whether ERMES directly facilitates the lipid exchange between the two organelles remains controversial. Here, we compared the x-ray structures of an ERMES subunit Mdm12 from Kluyveromyces lactis with that of Mdm12 from Saccharomyces cerevisiae and found that both Mdm12 proteins possess a hydrophobic pocket for phospholipid binding. However in vitro lipid transfer assays showed that Mdm12 alone or an Mmm1 (another ERMES subunit) fusion protein exhibited only a weak lipid transfer activity between liposomes. In contrast, Mdm12 in a complex with Mmm1 mediated efficient lipid transfer between liposomes. Mutations in Mmm1 or Mdm12 impaired the lipid transfer activities of the Mdm12-Mmm1 complex and furthermore caused defective phosphatidylserine transport from the ER to mitochondrial membranes via ERMES in vitro. Therefore, the Mmm1-Mdm12 complex functions as a minimal unit that mediates lipid transfer between membranes. © 2018 Kawano et al.

  3. Extended Rasch Modeling: The eRm Package for the Application of IRT Models in R

    Directory of Open Access Journals (Sweden)

    Patrick Mair

    2007-02-01

    Full Text Available Item response theory models (IRT are increasingly becoming established in social science research, particularly in the analysis of performance or attitudinal data in psychology, education, medicine, marketing and other fields where testing is relevant. We propose the R package eRm (extended Rasch modeling for computing Rasch models and several extensions. A main characteristic of some IRT models, the Rasch model being the most prominent, concerns the separation of two kinds of parameters, one that describes qualities of the subject under investigation, and the other relates to qualities of the situation under which the response of a subject is observed. Using conditional maximum likelihood (CML estimation both types of parameters may be estimated independently from each other. IRT models are well suited to cope with dichotomous and polytomous responses, where the response categories may be unordered as well as ordered. The incorporation of linear structures allows for modeling the effects of covariates and enables the analysis of repeated categorical measurements. The eRm package fits the following models: the Rasch model, the rating scale model (RSM, and the partial credit model (PCM as well as linear reparameterizations through covariate structures like the linear logistic test model (LLTM, the linear rating scale model (LRSM, and the linear partial credit model (LPCM. We use an unitary, efficient CML approach to estimate the item parameters and their standard errors. Graphical and numeric tools for assessing goodness-of-fit are provided.

  4. TcdC does not significantly repress toxin expression in Clostridium difficile 630ΔErm.

    Directory of Open Access Journals (Sweden)

    Dennis Bakker

    Full Text Available In the past decade, Clostridium difficile has emerged as an important gut pathogen. Symptoms of C. difficile infection range from mild diarrhea to pseudomembranous colitis, sometimes resulting in colectomy or death. The main virulence factors of C. difficile are toxin A and toxin B. Besides the genes encoding these toxins (tcdA and tcdB, the pathogenicity locus (PaLoc also contains genes encoding a sigma factor (tcdR and a putative anti-sigma factor (tcdC. The important role of TcdR as a sigma factor for toxin expression is undisputed, whereas the role of TcdC as an anti-sigma factor, inhibiting toxin expression, is currently the subject of debate. To clarify the role of TcdC in toxin expression, we generated an isogenic ClosTron-based mutant of tcdC in Clostridium difficile strain 630Δ Erm (CT::tcdC and determined the transcription levels of the PaLoc genes and the expression levels of the toxins in the wild type strain and the tcdC mutant strain. We found only minor differences in transcription levels of the PaLoc genes between the wild type and CT::tcdC strains and total toxin levels did not significantly differ either. These results suggest that in C. difficile 630Δerm TcdC is not a major regulator of toxin expression under the conditions tested.

  5. The New Macrolide-Lincosamide-Streptogramin B Resistance Gene erm(45) Is Located within a Genomic Island in Staphylococcus fleurettii

    DEFF Research Database (Denmark)

    Wipf, Juliette R K; Schwendener, Sybille; Nielsen, Jesper Boye

    2015-01-01

    Genome alignment of a macrolide, lincosamide, and streptogramin B (MLSB)-resistant Staphylococcus fleurettii strain with an MLSB-susceptible S. fleurettii strain revealed a novel 11,513-bp genomic island carrying the new erythromycin resistance methylase gene erm(45). This gene was shown to confe...... inducible MLSB resistance when cloned into Staphylococcus aureus. The erm(45)-containing island was integrated into the housekeeping gene guaA in S. fleurettii and was able to form a circular intermediate but was not transmissible to S. aureus....

  6. Oral and anal vaccination confers full protection against enteric redmouth disease (ERM in rainbow trout.

    Directory of Open Access Journals (Sweden)

    Kasper Rømer Villumsen

    Full Text Available The effect of oral vaccines against bacterial fish diseases has been a topic for debate for decades. Recently both M-like cells and dendritic cells have been discovered in the intestine of rainbow trout. It is therefore likely that antigens reaching the intestine can be taken up and thereby induce immunity in orally vaccinated fish. The objective of this project was to investigate whether oral and anal vaccination of rainbow trout induces protection against an experimental waterborne infection with the pathogenic enterobacteria Yersinia ruckeri O1 biotype 1 the causative agent of enteric redmouth disease (ERM. Rainbow trout were orally vaccinated with AquaVac ERM Oral (MERCK Animal Health or an experimental vaccine bacterin of Y. ruckeri O1. Both vaccines were tested with and without a booster vaccination four months post the primary vaccination. Furthermore, two groups of positive controls were included, one group receiving the experimental oral vaccine in a 50 times higher dose, and the other group receiving a single dose administered anally in order to bypass the stomach. Each group was bath challenged with 6.3 × 10(8 CFU/ml Y. ruckeri, six months post the primary vaccination. The challenge induced significant mortality in all the infected groups except for the groups vaccinated anally with a single dose or orally with the high dose of bacterin. Both of these groups had 100% survival. These results show that a low dose of Y. ruckeri bacterin induces full protection when the bacterin is administered anally. Oral vaccination also induces full protection, however, at a dose 50 times higher than if the fish were to be vaccinated anally. This indicates that much of the orally fed antigen is digested in the stomach before it reaches the second segment of the intestine where it can be taken up as immunogenic antigens and presented to lymphocytes.

  7. Telithromycin resistance in Streptococcus pneumoniae is conferred by a deletion in the leader sequence of erm(B) that increases rRNA methylation

    DEFF Research Database (Denmark)

    Wolter, Nicole; Smith, Anthony M; Farrell, David J

    2008-01-01

    A telithromycin-resistant clinical isolate of Streptococcus pneumoniae (strain P1501016) has been found to contain a version of erm(B) that is altered by a 136-bp deletion in the leader sequence. By allele replacement mutagenesis, a second strain of S. pneumoniae (PC13) with a wild-type erm(B) ge...

  8. Resistance determinant erm(X) is borne by transposon Tn5432 in Bifidobacterium thermophilum and Bifidobacterium animals subsp. lactis

    NARCIS (Netherlands)

    Hoek, van A.H.A.M.; Mayrhofer, S.; Domig, K.J.; Aarts, H.J.M.

    2008-01-01

    The erm(X) gene from erythromycin- and clindamycin-resistant Bifidobacterium strains was characterised by polymerase chain reaction and sequence analysis, including flanking regions. Results suggest that the resistance determinant was part of transposon Tn5432 that has been described in several

  9. Genome Comparison of Erythromycin Resistant Campylobacter from Turkeys Identifies Hosts and Pathways for Horizontal Spread of erm(B Genes

    Directory of Open Access Journals (Sweden)

    Diego Florez-Cuadrado

    2017-11-01

    Full Text Available Pathogens in the genus Campylobacter are the most common cause of food-borne bacterial gastro-enteritis. Campylobacteriosis, caused principally by Campylobacter jejuni and Campylobacter coli, is transmitted to humans by food of animal origin, especially poultry. As for many pathogens, antimicrobial resistance in Campylobacter is increasing at an alarming rate. Erythromycin prescription is the treatment of choice for clinical cases requiring antimicrobial therapy but this is compromised by mobility of the erythromycin resistance gene erm(B between strains. Here, we evaluate resistance to six antimicrobials in 170 Campylobacter isolates (133 C. coli and 37 C. jejuni from turkeys. Erythromycin resistant isolates (n = 85; 81 C. coli and 4 C. jejuni were screened for the presence of the erm(B gene, that has not previously been identified in isolates from turkeys. The genomes of two positive C. coli isolates were sequenced and in both isolates the erm(B gene clustered with resistance determinants against aminoglycosides plus tetracycline, including aad9, aadE, aph(2″-IIIa, aph(3′-IIIa, and tet(O genes. Comparative genomic analysis identified identical erm(B sequences among Campylobacter from turkeys, Streptococcus suis from pigs and Enterococcus faecium and Clostridium difficile from humans. This is consistent with multiple horizontal transfer events among different bacterial species colonizing turkeys. This example highlights the potential for dissemination of antimicrobial resistance across bacterial species boundaries which may compromise their effectiveness in antimicrobial therapy.

  10. Genome Comparison of Erythromycin Resistant Campylobacter from Turkeys Identifies Hosts and Pathways for Horizontal Spread of erm(B) Genes.

    Science.gov (United States)

    Florez-Cuadrado, Diego; Ugarte-Ruiz, María; Meric, Guillaume; Quesada, Alberto; Porrero, M C; Pascoe, Ben; Sáez-Llorente, Jose L; Orozco, Gema L; Domínguez, Lucas; Sheppard, Samuel K

    2017-01-01

    Pathogens in the genus Campylobacter are the most common cause of food-borne bacterial gastro-enteritis. Campylobacteriosis, caused principally by Campylobacter jejuni and Campylobacter coli, is transmitted to humans by food of animal origin, especially poultry. As for many pathogens, antimicrobial resistance in Campylobacter is increasing at an alarming rate. Erythromycin prescription is the treatment of choice for clinical cases requiring antimicrobial therapy but this is compromised by mobility of the erythromycin resistance gene erm(B) between strains. Here, we evaluate resistance to six antimicrobials in 170 Campylobacter isolates (133 C. coli and 37 C. jejuni) from turkeys. Erythromycin resistant isolates (n = 85; 81 C. coli and 4 C. jejuni) were screened for the presence of the erm(B) gene, that has not previously been identified in isolates from turkeys. The genomes of two positive C. coli isolates were sequenced and in both isolates the erm(B) gene clustered with resistance determinants against aminoglycosides plus tetracycline, including aad9, aadE, aph(2″)-IIIa, aph(3')-IIIa, and tet(O) genes. Comparative genomic analysis identified identical erm(B) sequences among Campylobacter from turkeys, Streptococcus suis from pigs and Enterococcus faecium and Clostridium difficile from humans. This is consistent with multiple horizontal transfer events among different bacterial species colonizing turkeys. This example highlights the potential for dissemination of antimicrobial resistance across bacterial species boundaries which may compromise their effectiveness in antimicrobial therapy.

  11. Presence of erm genes among macrolide-resistant Gram-positive bacteria isolated from Danish farm soil

    DEFF Research Database (Denmark)

    Jensen, Lars Bogø; Agersø, Yvonne; Sengeløv, Gitte

    2002-01-01

    The presence of erm genes was investigated among macrolide-resistant Gram-positive bacteria isolated from soil samples collected from four Danish farms that had been treated with animal waste. Soil samples were collected before, a few days after spread and 1 1/2 months and 5 months later. In 33% (9...

  12. Opening a Can of wERMS: Texas A&M University's Experiences in Implementing Two Electronic Resource Management Systems

    Science.gov (United States)

    Hartnett, Eric; Price, Apryl; Smith, Jane; Barrett, Michael

    2010-01-01

    Over the past few years, Texas A&M University (TAMU) has searched for a way to administer its electronic subscriptions as well as the electronic subscriptions shared among the TAMU System. In this article, we address our attempts to implement an effective electronic resource management system (ERMS), both for subscriptions on the main campus…

  13. Standardized interpretation of antibiotic susceptibility testing and resistance genotyping for Mycobacterium abscessus with regard to subspecies and erm41 sequevar.

    Science.gov (United States)

    Mougari, Faiza; Amarsy, Rishma; Veziris, Nicolas; Bastian, Sylvaine; Brossier, Florence; Berçot, Béatrice; Raskine, Laurent; Cambau, Emmanuelle

    2016-08-01

    The objective of this study was to provide standardized antibiotic susceptibility testing (AST) for Mycobacterium abscessus with regard to subspecies. One hundred and sixty-five clinical isolates were tested for susceptibility to 15 antibiotics using a commercial microdilution method, at two reading times: (i) early reading time (ERT), when the growth control was first positive; and (ii) late reading time (LRT), of 14 days, for detecting inducible resistance. In addition, genes or mutations involved in resistance were studied [erm(41), rrl and rrs]. Three patterns were observed for clarithromycin: (i) MIC >16 mg/L at ERT (median 5 days) for 15 isolates [10 subsp. abscessus erm(41) sequevar T28, 3 subsp. bolletii and 2 subsp. massiliense] among which 9 harboured an a2058g/c rrl mutation; (ii) MIC ≤16 mg/L at ERT, but >16 mg/L at LRT, for 106 isolates [84 abscessus erm(41) T28 and 22 bolletii] showing intrinsic inducible resistance; and (iii) MIC ≤4 mg/L at ERT and LRT for 44 isolates [18 abscessus erm(41) C28 and 26 massiliense]. Amikacin MIC was >64 mg/L for eight isolates [five abscessus erm(41) T28, two massiliense and one bolletii] among which seven harboured the a1408g rrs mutation, but ≤64 mg/L for the remaining isolates without mutation. For the other antibiotics, only one WT pattern was observed, with cefoxitin, tigecycline and linezolid showing MIC values compatible with susceptibility. Standard AST can predict clarithromycin and amikacin resistance using interpretation rules with regard to subspecies. For other antibiotics, since only one pattern is observed, there is no need for systematic phenotypic or genotypic testing. © The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  14. Conjugative transfer of the erm(A) gene from erythromycin-resistant Streptococcus pyogenes to macrolide-susceptible S. pyogenes, Enterococcus faecalis and Listeria innocua.

    Science.gov (United States)

    Giovanetti, E; Magi, G; Brenciani, A; Spinaci, C; Lupidi, R; Facinelli, B; Varaldo, P E

    2002-08-01

    In mating experiments, the erythromycin resistance methylase gene erm(A) was successfully transferred from erm(A)-positive clinical isolates of Streptococcus pyogenes to macrolide-susceptible recipients of S. pyogenes, Enterococcus faecalis and Listeria innocua. Compared with the SmaI macrorestriction pattern of the S. pyogenes recipient, the patterns of S. pyogenes transconjugants shared the lack of a fragment and the appearance of a new, larger fragment. This is the first experimental evidence that the erm(A) gene can be transferred from erythromycin-resistant S. pyogenes to macrolide-susceptible S. pyogenes as well as to other Gram-positive recipients.

  15. DEVELOPMENT OF A YERSINIA RUCKERI O1 BIOTYPE 2 WATERBORNE CHALLENGE MODEL FOR FUTURE ERM VACCINE TESTING IN RAINBOW TROUT

    DEFF Research Database (Denmark)

    Kragelund Strøm, Helene; Aalbæk, Bent; Otani, Maki

    for more than 35 years. Recent years have shown ERM outbreaks caused by Y.r. O1 bt 2 reported from ERM vaccinated farmed rainbow trout stocks in both Europe and the USA. It has been suggested that Y.r. O1 bt 2 is more virulent than bt 1. In order to test this hypothesis we have collected Y.r. O1 isolates...... causing less than 50 % mortality, and a high virulence group causing more than 50 % mortality. Interestingly, all tested bt 1 isolates grouped in the low virulence group together with a few bt 2 isolates. The highly virulent strains were exclusively Y.r. O1 bt 2 strains. The most virulent strain......1 bt 2 has made it possible to develop a standardized bath challenge model which is needed in order to test and develop new experimental vaccines which are expected to induce immunity against Y.r. O1 bt 2....

  16. Geodetic data records (GDRs) from the GEOSAT Exact Repeat Mission (ERM) from 27 April 1987 to 30 May 1987 (NODC Accession 8700243)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains one month of geodetic data records (GDRs) from the GEOSAT Exact Repeat Mission (ERM) for the time period of April 27, 1987 to May 30, 1987....

  17. Crossover difference data records (XDR) from GEOSAT Geodetic Mission (GM) and Exact Repeat Mission (ERM) data from 01 April 1985 to 07 November 1987 (NODC Accession 9000213)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Crossover difference data records (XDRs) from the GEOSAT Geodetic Mission (GM) and Exact Repeat Mission (ERM) for the time period of April 1, 1985 to November 7,...

  18. Geodetic data records (GDRs) from the GEOSAT Exact Repeat Mission (ERM) from 07 August 1987 to 09 September 1987 (NODC Accession 8700339)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains one month of geodetic data records (GDRs) from the GEOSAT Exact Repeat Mission (ERM) for the time period of August 07, 1987 to September 09,...

  19. Geodetic data records (GDRs) from the GEOSAT Exact Repeat Mission (ERM) from 02 November 1986 to 12 December 1986 (NODC Accession 8700069)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains one month of geodetic data records (GDRs) from the GEOSAT Exact Repeat Mission (ERM) for the time period of November 02, 1986 to December 12,...

  20. Geodetic data records (GDRs) from the GEOSAT Exact Repeat Mission (ERM) from 14 August 1987 to 16 November 1987 (NODC Accession 8800001)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains one month of geodetic data records (GDRs) from the GEOSAT Exact Repeat Mission (ERM) for the time period of August 14, 1987 to November 16,...

  1. Time-to-Detection of Inducible Macrolide Resistance in Mycobacterium abscessus Subspecies and Its Association with the Erm(41 Sequevar.

    Directory of Open Access Journals (Sweden)

    Sara Christianson

    Full Text Available Mutations in the erm(41 gene of M.abscessus group organisms are associated with differences in inducible macrolide resistance, with current recommendations being to hold rapidly growing isolates for up to 14 days in order to ensure that resistance which develops more slowly can be detected. This study aimed to determine the ideal incubation time for accurate identification of inducible macrolide resistance as well as to determine if there was an association between the time taken to detect inducible resistance in M.abscessus group organisms and their erm(41 sequevar. We amplified and sequenced the erm(41 genes of a total of 104 M.abscessus group isolates and determined their sequevars. The isolates were tested for phenotypic clarithromycin resistance at days 7, 10, 14 and 21, using Trek Diagnostics Sensititre RAPMYCO microbroth dilution plates. Associations between erm(41 gene sequevars and time to detection of resistance were evaluated using Fisher's exact test in R. The samples included in this study fell into 14 sequevars, with the majority of samples falling into Sequevar02 (16, Sequevar06 (15, Sequevar08 (7 and Sequvar 15 (31, and several isolates that were in small clusters, or unique. The majority (82.7% of samples exhibiting inducible macrolide resistance were interpreted as resistant by day 7. Two isolates in Sequevar02, which has a T28C mutation that is associated with sensitivity, showed intermediate resistance at day 14, though the majority (13 were sensitive at day 14. The majority of isolates with inducible macrolide resistance fell into Sequevars 06,08 and 15, none of which contain the T28C mutation. These sequevars were analyzed to determine if there was any correlation between sequevar and time to detection of resistance. None was found. Based on these findings, we recommend the addition of a day 7 read to the CLSI guidelines to improve turn-around-times for these isolates. It is also recommended that erm(41 gene sequencing be

  2. Advanced order management in ERM systems: the tic-tac-toe algorithm

    Science.gov (United States)

    Badell, Mariana; Fernandez, Elena; Puigjaner, Luis

    2000-10-01

    The concept behind improved enterprise resource planning systems (ERP) systems is the overall integration of the whole enterprise functionality into the management systems through financial links. Converting current software into real management decision tools requires crucial changes in the current approach to ERP systems. This evolution must be able to incorporate the technological achievements both properly and in time. The exploitation phase of plants needs an open web-based environment for collaborative business-engineering with on-line schedulers. Today's short lifecycles of products and processes require sharp and finely tuned management actions that must be guided by scheduling tools. Additionally, such actions must be able to keep track of money movements related to supply chain events. Thus, the necessary outputs require financial-production integration at the scheduling level as proposed in the new approach of enterprise management systems (ERM). Within this framework, the economical analysis of the due date policy and its optimization become essential to manage dynamically realistic and optimal delivery dates with price-time trade-off during the marketing activities. In this work we propose a scheduling tool with web-based interface conducted by autonomous agents when precise economic information relative to plant and business actions and their effects are provided. It aims to attain a better arrangement of the marketing and production events in order to face the bid/bargain process during e-commerce. Additionally, management systems require real time execution and an efficient transaction-oriented approach capable to dynamically adopt realistic and optimal actions to support marketing management. To this end the TicTacToe algorithm provides sequence optimization with acceptable tolerances in realistic time.

  3. Crystal structure of Mdm12 and combinatorial reconstitution of Mdm12/Mmm1 ERMES complexes for structural studies

    Energy Technology Data Exchange (ETDEWEB)

    AhYoung, Andrew P.; Lu, Brian; Cascio, Duilio; Egea, Pascal F.

    2017-06-01

    Membrane contact sites between organelles serve as molecular hubs for the exchange of metabolites and signals. In yeast, the Endoplasmic Reticulum – Mitochondrion Encounter Structure (ERMES) tethers these two organelles likely to facilitate the non-vesicular exchange of essential phospholipids. Present in Fungi and Amoebas but not in Metazoans, ERMES is composed of five distinct subunits; among those, Mdm12, Mmm1 and Mdm34 each contain an SMP domain functioning as a lipid transfer module. We previously showed that the SMP domains of Mdm12 and Mmm1 form a hetero-tetramer. Here we describe our strategy to diversify the number of Mdm12/Mmm1 complexes suited for structural studies. We use sequence analysis of orthologues combined to protein engineering of disordered regions to guide the design of protein constructs and expand the repertoire of Mdm12/Mmm1 complexes more likely to crystallize. Using this combinatorial approach we report crystals of Mdm12/Mmm1 ERMES complexes currently diffracting to 4.5 Å resolution and a new structure of Mdm12 solved at 4.1 Å resolution. Our structure reveals a monomeric form of Mdm12 with a conformationally dynamic N-terminal β-strand; it differs from a previously reported homodimeric structure where the N-terminal β strands where swapped to promote dimerization. Based on our electron microscopy data, we propose a refined pseudo-atomic model of the Mdm12/Mmm1 complex that agrees with our crystallographic and small-angle X-ray scattering (SAXS) solution data.

  4. Crystal structure of Mdm12 and combinatorial reconstitution of Mdm12/Mmm1 ERMES complexes for structural studies.

    Science.gov (United States)

    AhYoung, Andrew P; Lu, Brian; Cascio, Duilio; Egea, Pascal F

    2017-06-17

    Membrane contact sites between organelles serve as molecular hubs for the exchange of metabolites and signals. In yeast, the Endoplasmic Reticulum - Mitochondrion Encounter Structure (ERMES) tethers these two organelles likely to facilitate the non-vesicular exchange of essential phospholipids. Present in Fungi and Amoebas but not in Metazoans, ERMES is composed of five distinct subunits; among those, Mdm12, Mmm1 and Mdm34 each contain an SMP domain functioning as a lipid transfer module. We previously showed that the SMP domains of Mdm12 and Mmm1 form a hetero-tetramer. Here we describe our strategy to diversify the number of Mdm12/Mmm1 complexes suited for structural studies. We use sequence analysis of orthologues combined to protein engineering of disordered regions to guide the design of protein constructs and expand the repertoire of Mdm12/Mmm1 complexes more likely to crystallize. Using this combinatorial approach we report crystals of Mdm12/Mmm1 ERMES complexes currently diffracting to 4.5 Å resolution and a new structure of Mdm12 solved at 4.1 Å resolution. Our structure reveals a monomeric form of Mdm12 with a conformationally dynamic N-terminal β-strand; it differs from a previously reported homodimeric structure where the N-terminal β strands where swapped to promote dimerization. Based on our electron microscopy data, we propose a refined pseudo-atomic model of the Mdm12/Mmm1 complex that agrees with our crystallographic and small-angle X-ray scattering (SAXS) solution data. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. A combined cryo-EM and molecular dynamics approach reveals the mechanism of ErmBL-mediated translation arrest

    Science.gov (United States)

    Arenz, Stefan; Bock, Lars V.; Graf, Michael; Innis, C. Axel; Beckmann, Roland; Grubmüller, Helmut; Vaiana, Andrea C.; Wilson, Daniel N.

    2016-07-01

    Nascent polypeptides can induce ribosome stalling, regulating downstream genes. Stalling of ErmBL peptide translation in the presence of the macrolide antibiotic erythromycin leads to resistance in Streptococcus sanguis. To reveal this stalling mechanism we obtained 3.6-Å-resolution cryo-EM structures of ErmBL-stalled ribosomes with erythromycin. The nascent peptide adopts an unusual conformation with the C-terminal Asp10 side chain in a previously unseen rotated position. Together with molecular dynamics simulations, the structures indicate that peptide-bond formation is inhibited by displacement of the peptidyl-tRNA A76 ribose from its canonical position, and by non-productive interactions of the A-tRNA Lys11 side chain with the A-site crevice. These two effects combine to perturb peptide-bond formation by increasing the distance between the attacking Lys11 amine and the Asp10 carbonyl carbon. The interplay between drug, peptide and ribosome uncovered here also provides insight into the fundamental mechanism of peptide-bond formation.

  6. Gaboni riigipea lahkub presidendipaleest vaid jalad ees / Allan Espenberg

    Index Scriptorium Estoniae

    Espenberg, Allan

    2005-01-01

    Kesk-Aafrika riigi Gaboni president Omar Bongo püüab detsembris toimuvatel presidendivalimistel taotleda enda tagasivalimist. Bongo on juhtinud Gaboni 38 aastat, olles sellega Aafrika riigipeade hulgas kõige staazhikam valitseja

  7. Valel on lühikesed jalad / Meelis Atonen

    Index Scriptorium Estoniae

    Atonen, Meelis, 1966-

    2001-01-01

    Haldusterritoriaalse reformi kriitika. Autor: Reformierakond. Parlamendisaadik. Ilmunud ka: Koit, 17. mai 2001, lk. 6; Sakala, 22. mai 2001, lk. 2; Võrumaa Teataja, 22. mai 2001, lk. 2; Paremad Uudised, mai, nr. 11, lk. 2; Valgamaalane, 29. mai 2001, lk. 2

  8. Los riesgos de las entidades aseguradoras en el marco del Enterprise Risk Management (ERM y el control interno

    Directory of Open Access Journals (Sweden)

    Rafael Hernández Barros

    2015-12-01

    Full Text Available Los sistemas integrales de gestión del riesgo, en particular el Enterprise Risk Management (ERM, se han convertido en una práctica fundamental de la gestión de empresas para la mitigación y control de los riesgos. La crisis financiera actual ha estimulado a las empresas aseguradoras a dar mayor importancia a la gestión de los riesgos, alentadas además por la nueva regulación europea Solvencia II; por ello el objetivo principal de este trabajo ha sido proponer un marco conceptual de los riesgos a los que se enfrentan las empresas internacionales de seguros desde una perspectiva global, cuya actividad presenta peculiaridades específicas que hacen que el proceso de control interno y gestión de riesgos adquieran una especial relevancia, y que puede ser aplicado a empresas de otros sectores económicos.

  9. Charakterisierung und Bewertung der Zug- und Ermüdungseigenschaften von Mikrobauteilen aus 17-4PH Edelstahl - Ein Vergleich von mikropulverspritzgegossenem und konventionell hergestelltem Material

    OpenAIRE

    Slaby, S.A.

    2015-01-01

    Im Rahmen der vorliegenden Arbeit wurde ein Mikro-Testverfahren entwickelt, mit dem die Zug- und Ermüdungseigenschaften von mikropulverspritzgegossenen (µPIM) Bauteilen aus 17 4PH Edelstahl gemessen wurden. Der Vergleich mit konventionell hergestelltem Material zeigte, dass das µPIM Material bereits gute mechanische Eigenschaften aufweist, im Bereich der Mikrostruktur aber noch Optimierungsbedarf besteht.

  10. Charakterisierung und Bewertung der Zug- und Ermüdungseigenschaften von Mikrobauteilen aus 17-4PH Edelstahl - Ein Vergleich von mikropulverspritzgegossenem und konventionell hergestelltem Material

    OpenAIRE

    Slaby, Stefan Andreas

    2017-01-01

    Im Rahmen der vorliegenden Arbeit wurde ein Mikro-Testverfahren entwickelt, mit dem die Zug- und Ermüdungseigenschaften von mikropulverspritzgegossenen (µPIM) Bauteilen aus 17 4PH Edelstahl gemessen wurden. Der Vergleich mit konventionell hergestelltem Material zeigte, dass das µPIM Material bereits gute mechanische Eigenschaften aufweist, im Bereich der Mikrostruktur aber noch Optimierungsbedarf besteht.

  11. Põhja-Ameerika looderanniku indiaanlaste kultuur : nahkrõivad ja nende valmistamine. Nahast puusapõlle ERM C 19:27ab konserveerimine / Tea Šumanov

    Index Scriptorium Estoniae

    Šumanov, Tea

    2011-01-01

    Antakse ülevaade Põhja-Ameerika indiaanlaste kultuurist ja suhestumisest loodusega, kirjeldatakse nende nahakäsitöö ja kaunistamise meetodeid. Etnograafiliste museaalide väärtusest, nende kahjustustest ning konserveerimiseks valitud meetoditest ning ERM-i eksponaadi konserveerimise praktilisest protsessist

  12. Kuidas peaks õpetaja karistama ülekäte läinud õpilast? / Aage Õunap, Toomas Palu, Aivar Haller, Maia Erm

    Index Scriptorium Estoniae

    2008-01-01

    Haridusministri hinnangul peaks koolil olema õigus korda rikkuvaid õpilasi trahvida. Oma arvamust selle kohta avaldavad Viljandimaa Lasterikaste Perede Ühenduse juht Aage Õunap, UNICEF Eesti rahvuskomitee esindaja Toomas Palu, Eesti Lastevanemate Liidu juhatuse liige Aivar Haller ja Pärnu Koidula Gümnaasiumi õppealajuhataja Maia Erm

  13. Xijiao Dihuang Decoction combined with Yinqiao Powder reverses influenza virus-induced F-actin reorganization in PMVECs by inhibiting ERM phosphorylation

    Directory of Open Access Journals (Sweden)

    Zinan Xuan

    2016-01-01

    Conclusion: These results show that XDY-CS inhibited influenza-induced F-actin reorganization in PMVECs by down-regulating p-ERM expression via inhibition of the Rho/ROCK, p38 MAPK, and PKC pathways. In conclusion, XDY could reduce the damage to endothelial cytoskeleton induced by IV infection, thus protecting the barriers of PMVECs.

  14. Listeria monocytogenes isolates from food and food environment harbouring tetM and ermB resistance genes.

    Science.gov (United States)

    Haubert, L; Mendonça, M; Lopes, G V; de Itapema Cardoso, M R; da Silva, W P

    2016-01-01

    Listeria monocytogenes is a foodborne pathogen that has become an important cause of human and animal diseases worldwide. The purpose of this study was to evaluate the serotypes, virulence potential, antimicrobial resistance profile, and genetic relationships of 50 L. monocytogenes isolates from food and food environment in southern Brazil. In this study, the majority of L. monocytogenes isolates belonged to the serotypes 1/2b (42%) and 4b (26%), which are the main serotypes associated with human listeriosis. In addition, all isolates harboured internalin genes (inlA, inlC, inlJ), indicating a virulence potential. The isolates were sensitive to most of the antimicrobial compounds analysed, and five isolates (10%) were multi-resistant. Two isolates harboured antimicrobial resistance genes (tetM and ermB) and in one of them, the gene was present in the plasmid. Moreover, according to the pulsed field gel electrophoresis assay, two multi-resistant isolates were a single clone isolated from food and the processing plant. The isolates were susceptible to the most frequently used antibiotics for listeriosis treatment. However, the presence of multidrug-resistant isolates and antimicrobial resistance genes including in the plasmid could even be transferred between bacterial species, suggesting a potential health risk to consumers and a potential risk of spreading multi-resistance genes to other bacteria. Listeria monocytogenes is an important agent of foodborne diseases. The results of this study suggest a potential capacity of L. monocytogenes isolates from food and food environment to cause human infections. Antimicrobial multi-resistance profiles were detected in 10%, and two isolates harboured tetM and ermB resistance genes. Moreover, the present research can help to build up a better knowledge about antimicrobial resistance of L. monocytogenes. Additionally, we found one isolate carrying tetM resistance gene in a plasmid, that suggests a possible transmission

  15. Summary Describing Integration of ERM Methodology into Supervisory Control Framework with Software Package Documentation; Advanced Reactor Technology Milestone: M4AT-16PN2301052

    Energy Technology Data Exchange (ETDEWEB)

    Ramuhalli, Pradeep [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hirt, Evelyn H. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Dib, Gerges [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Veeramany, Arun [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Bonebrake, Christopher A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Roy, Surajit [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-09-20

    This project involved the development of enhanced risk monitors (ERMs) for active components in Advanced Reactor (AdvRx) designs by integrating real-time information about equipment condition with risk monitors. Health monitoring techniques in combination with predictive estimates of component failure based on condition and risk monitors can serve to indicate the risk posed by continued operation in the presence of detected degradation. This combination of predictive health monitoring based on equipment condition assessment and risk monitors can also enable optimization of maintenance scheduling with respect to the economics of plant operation. This report summarizes PNNL’s multi-year project on the development and evaluation of an ERM concept for active components while highlighting FY2016 accomplishments. Specifically, this report provides a status summary of the integration and demonstration of the prototypic ERM framework with the plant supervisory control algorithms being developed at Oak Ridge National Laboratory (ORNL), and describes additional case studies conducted to assess sensitivity of the technology to different quantities. Supporting documentation on the software package to be provided to ONRL is incorporated in this report.

  16. Sieviešu ķermeņa disciplīnas reprezentācija žurnālā "Cosmopolitan" (2002–2013)

    OpenAIRE

    Jekimova, Jekaterina

    2014-01-01

    Šī bakalaura darba tēma ir “Sieviešu ķermeņa disciplīnas reprezentācija žurnālā “Cosmopolitan” (2002-2013)”. Pētījuma problēma: kāda nozīme tika piešķirta sieviešu ķermeņa disciplīnām starptautiskās Latvijas versijas žurnālā “Cosmopolitan”. Šī darba mērķis ir noskaidrot šo disciplīnu reprezentāciju izvelētājā preses izdevumā. Teorētiskajā daļā tika aplūkota reprezentācijas teorija, sociālās mācīšanās teorija, apskatīts kopējais sievietes tēls populārajā kultūrā, sieviešu ķermeņa disciplīna...

  17. Atšķirības sieviešu ar augstu un zemu ķermeņa pašvērtējumu spriedumos par sabiedrībā populāru sieviešu ķermeņa izmēriem.

    OpenAIRE

    Kalniņa, Aija

    2009-01-01

    Pētījuma mērķis bija noskaidrot, vai pastāv atšķirības ar savu ķermeni neapmierinātu sieviešu un ar savu ķermeni apmierinātu sieviešu spriedumos par sabiedrībā populāru un medijos bieži redzamu sieviešu ķermeņu izmēriem. Pētījumā piedalījās divas izlases grupas: vienā bija 24 sievietes ar augstu ķermeņa pašvērtējumu, otrā – 25 sievietes ar zemu ķermeņa pašvērtējumu. Sievietes bija vecumā no 18 līdz 25 gadiem, kuru ķermeņa masas indekss (ĶMI) iekļaujas normālos rādītājos, un kurām nav ēšana...

  18. Pašcieņas, ķermeņa tēla un akadēmisko sasniegumu saistība pusaudžiem

    OpenAIRE

    Skvorcova, Beāte

    2015-01-01

    Bakalaura darba tēma ir pašcieņas, ķermeņa tēla un akadēmisko sasniegumu saistība pusaudžiem. Darba mērķis ir pētīt saistības starp pašcieņu, ķermeņa tēlu un akadēmiskajiem sasniegumiem pusaudžiem. Darba teorētiskajā daļā tiek aprakstīts pusaudžu vecumposma raksturojums, pašcieņa Pusaudžiem un ķermeņa tēls pusaudžiem. Atsevišķi tiek aprakstīta pašcieņa un ķermeņa tēls, pašcieņa un akadēmiskie sasniegumi. Praktiskajā daļā tiek pētītas pašcieņas, ķermeņa tēla un akadēmisko sasnie...

  19. Phylogeography of Yersinia ruckeri reveals effects of past evolutionary events on the current strain distribution and explains variations in the global transmission of Enteric Redmouth (ERM disease.

    Directory of Open Access Journals (Sweden)

    Asmine eBastardo

    2015-10-01

    Full Text Available Phylogeographic patterns and population genetic structure of Yersinia ruckeri, the pathological agent of enteric redmouth disease (ERM in salmonids, were investigated on the basis of concatenated multiloci sequences from isolates of different phenotypes obtained between 1965-2009 from diverse areas and hosts. Sequence analyses revealed genetic differentiation among subpopulations with the largest genetic distance occurring between subpopulations of Europe and Canada and/or South America. Bayesian analysis indicated the presence of three ancestral population clusters. Mismatch distribution displayed signatures characteristic of changes in size due to demographic and spatial expansions in the overall Y. ruckeri population, and also in the geographically separate subpopulations. Furthermore, a weak signal of isolation by distance was determined. A significant positive correlation between genetic and geographical distances was observed. These results revealed that the population of Y. ruckeri has undergone both ancient and recent population changes that were probably induced by biogeography forces in the past and, much more recently, by adaptive processes forced by aquaculture expansion. These findings have important implications for future studies on Y. ruckeri population dynamics, on the potential role of genetic structure to explain variations in ERM transmission, and on the effect of past evolutionary events on current estimations of gene flow.

  20. Antimicrobial susceptibility profile and research of mec A and erm genes in coagulase-negative staphylococci isolated from platelet concentrates bags

    Directory of Open Access Journals (Sweden)

    Rosiéli Martini

    2017-04-01

    Full Text Available Abstract In recent years, several studies have described the clinical impact of bacterial infection associated with transfusion of platelet concentrates (PCs. Among the blood components, PCs are responsible for the highest rates of bacterial contamination as well as septic transfusion reactions. We assessed antimicrobial susceptibility profile, resistance to methicillin (MRCoNS, and resistance to macrolides, lincosamides and streptogramins of group B (MLSB of 16 coagulase-negative staphylococci (CoNS isolates from an investigation in 691 PCs bags. We then compared conventional and automated phenotypic methods, disc diffusion test (DD and VITEK(r 2, respectively as well as phenotypic and genotypic methods (Polymerase Chain Reaction - PCR. All CoNS were susceptible to vancomycin. The disc diffusion test characterized 18.75% as MRCoNS and 37.5% with inducible resistance to MLSB (iMLSB, and with VITEK(r 2, 6.3% and 31.25%, respectively. The mecA gene was detected in 18.75% and the erm gene in 31.25% of the isolates. In this study, we found equal percentage values between presence of the mecA gene by PCR and resistance to methicillin using cefoxitin by DD test, evidence of the erm gene by PCR, and iMLSB resistance by automation (VITEK(r 2. Moreover, we identified three strains with beta-lactamase overproduction, and the occurrence of a bigger mistake was verified when automation was compared with DD test. And we observed that D-test was the most reliable for the detection of iMLSB resistance in Staphylococcus sp.

  1. Pusaudžu meiteņu pašcieņa saistībā ar pubertāti, ķermeņa tēlu un vardarbības pieredzi

    OpenAIRE

    Lapsa, Līga

    2013-01-01

    Šī bakalaura darba mērķis ir noskaidrot saistības starp pašcieņu, pubertāti, Ķermeņa masas indeksu, ķermeņa tēlu un vardarbības pieredzi 11 – 14 gadus vecām Latvijas pusaudžu meitenēm. Pētījuma izlasi veido 60 meitenes. Pētījumā izmantota Rozenberga pašcieņas aptauja, Ķermeņa tēla uztveres aptauja, Bērnības traumu aptauja, kā arī jautājumi par meitenes vecumu, menstruāciju sākšanās vecumu, augumu un svaru. Pētījuma rezultāti rāda, ka pastāv statistiski nozīmīga atšķirība ķermeņa tēla rādītājo...

  2. IMMERSION AND BATH VACCINATION AGAINST ENTERIC REDMOUTH DISEASE (ERM) PROVIDES INSUFFICIENT PROTECTION AGAINST BATH CHALLENGE WITH YERSINIA RUCKERI O1 BIOTYPE 2

    DEFF Research Database (Denmark)

    Kragelund Strøm, Helene; Otani, Maki; Neumann, Lukas

    trout stock which had been vaccinated against both Y.r. bt 1 and 2 with a commercial “state of the art” immersion vaccine and boosted with a commercial ERM oral vaccine 4 month later. The newly isolated Y.r. bt2 strain has been used to develop a standardized challenge model which gives us...... the opportunity to test and evaluate the effect of both commercial and experimental vaccines against Y.r. bt2 in a waterborne infection experiment, mimicking a natural infection. We have developed an experimental immersion and bath vaccine containing equal amounts of the most immunogenic Y.r. bt 1 and 2 strains...... of the vaccine for 30 seconds as recommended by the manufacturer. As a positive control groups of trout were bath vaccinated for 5 minutes. Two months later was all groups bath challenged with Y.r. bt 2 in duplicate. The challenge data obtained in the present study have indicated that neither a “state of the art...

  3. ERM 593 Applied Project_Guidance for Reviewing and Approving a Waste Stream Profile in the Waste Compliance and Tracking System_Final_05-05-15

    Energy Technology Data Exchange (ETDEWEB)

    Elicio, Andy U. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-05-05

    My ERM 593 applied project will provide guidance for the Los Alamos National Laboratory Waste Stream Profile reviewer (i.e. RCRA reviewer) in regards to Reviewing and Approving a Waste Stream Profile in the Waste Compliance and Tracking System. The Waste Compliance and Tracking system is called WCATS. WCATS is a web-based application that “supports the generation, characterization, processing and shipment of LANL radioactive, hazardous, and industrial waste.” The LANL generator must characterize their waste via electronically by filling out a waste stream profile (WSP) in WCATS. Once this process is completed, the designated waste management coordinator (WMC) will perform a review of the waste stream profile to ensure the generator has completed their waste stream characterization in accordance with applicable state, federal and LANL directives particularly P930-1, “LANL Waste Acceptance Criteria,” and the “Waste Compliance and Tracking System User's Manual, MAN-5004, R2,” as applicable. My guidance/applied project will describe the purpose, scope, acronyms, definitions, responsibilities, assumptions and guidance for the WSP reviewer as it pertains to each panel and subpanel of a waste stream profile.

  4. Evaluation of an experimental and commercial state-of-the-art vaccine against enteric redmouth disease (ERM) in rainbow trout by waterborne challenge with Y. ruckeri O1 biotype 2

    DEFF Research Database (Denmark)

    Kragelund Strøm, Helene; Otani, Maki; Villumsen, Kasper Rømer

    has been compared to a state-of-the-art commercial ERM immersion vaccine (AquaVac® ReleraTM). Un-vaccinated and sham vaccinated rainbow trout were included as controls. Two months post vaccination the rainbow trout were challenged in duplicate with Y. ruckeri O1 biotype 2 by bath. No effect...... of the experimental immersion or bath vaccine was observed in the present study. However, full protection was achieved with i.p. injection of the experimental vaccine (p

  5. Repulsive axon guidance cues ephrin-A2 and slit3 stop protrusion of the growth cone leading margin concurrently with inhibition of ADF/cofilin and ERM proteins.

    Science.gov (United States)

    Marsick, Bonnie M; Roche, Florence K; Letourneau, Paul C

    2012-07-01

    Axonal growth cones turn away from repulsive guidance cues. This may start with reduced protrusive motility in the region the growth cone leading margin that is closer to the source of repulsive cue. Using explants of E7 chick temporal retina, we examine the effects of two repulsive guidance cues, ephrin-A2 and slit3, on retinal ganglion cell growth cone protrusive activity, total F-actin, free F-actin barbed ends, and the activities (phosphorylation states) of actin regulatory proteins, ADF/cofilin and ezrin, radixin, moesin (ERM) proteins. Ephrin-A2 rapidly stops protrusive activity simultaneously with reducing F-actin, free barbed ends and the activities of ADF/cofilin and ERM proteins. Slit3 also stops protrusion and reduces the activities of ADF/cofilin and ERM proteins. We interpret these results as indicating that repulsive guidance cues inhibit actin polymerization and actin-membrane linkage to stop protrusive activity. Retrograde F-actin flow withdraws actin to the C-domain, where F-actin bundles interact with myosin II to generate contractile forces that can collapse and retract the growth cone. Our results suggest that common mechanisms are used by repulsive guidance cue to disable growth cone motility and remodel growing axon terminals. Copyright © 2012 Wiley Periodicals, Inc.

  6. C. difficile 630Δerm Spo0A regulates sporulation, but does not contribute to toxin production, by direct high-affinity binding to target DNA.

    Directory of Open Access Journals (Sweden)

    Katharina E Rosenbusch

    Full Text Available Clostridium difficile is a Gram positive, anaerobic bacterium that can form highly resistant endospores. The bacterium is the causative agent of C. difficile infection (CDI, for which the symptoms can range from a mild diarrhea to potentially fatal pseudomembranous colitis and toxic megacolon. Endospore formation in Firmicutes, including C. difficile, is governed by the key regulator for sporulation, Spo0A. In Bacillus subtilis, this transcription factor is also directly or indirectly involved in various other cellular processes. Here, we report that C. difficile Spo0A shows a high degree of similarity to the well characterized B. subtilis protein and recognizes a similar binding sequence. We find that the laboratory strain C. difficile 630Δerm contains an 18bp-duplication near the DNA-binding domain compared to its ancestral strain 630. In vitro binding assays using purified C-terminal DNA binding domain of the C. difficile Spo0A protein demonstrate direct binding to DNA upstream of spo0A and sigH, early sporulation genes and several other putative targets. In vitro binding assays suggest that the gene encoding the major clostridial toxin TcdB may be a direct target of Spo0A, but supernatant derived from a spo0A negative strain was no less toxic towards Vero cells than that obtained from a wild type strain, in contrast to previous reports. These results identify for the first time direct (putative targets of the Spo0A protein in C. difficile and make a positive effect of Spo0A on production of the large clostridial toxins unlikely.

  7. Reimaa lahkumine paneb Jõhvis võimuliidu püsimise proovile / Erik Gamzejev

    Index Scriptorium Estoniae

    Gamzejev, Erik, 1967-

    2007-01-01

    Ilmunud ka: Severnoje Poberezhje, 31. märts 2007, lk. 1. Vallo Reimaa asumine ministriametisse võib kaasa tuua jõujoonte muutuse Jõhvi vallavolikogus, kus võimuliidul on opositsiooni üle praegu vaid ühekohaline enamus. Lisa: Jõudude vahekord Jõhvi volikogus

  8. Järgmisel aastal pannakse mehitamata autode võimed linnas proovile / Erik Aru

    Index Scriptorium Estoniae

    Aru, Erik

    2006-01-01

    Defense Advanced Research Projects Agency korraldab 2007. aastal kolmandat korda ilma juhita autode võistluse. Projekti Grand Challenge raames pannakse ilma juhita autod sõitma esmakordselt linnas, küll mitte reaalses, vaid USA lääneosas loodud tehislinnas

  9. Comparison of heat balance- (Čermák and heat dissipation- (Granier derived sap flow measurements in ring-porous oaks and a pine species

    Directory of Open Access Journals (Sweden)

    Heidi J. Renninger

    2012-05-01

    Full Text Available Sap flow measurements have become integral in many physiological and ecological investigations. A number of methods are used to estimate sap flow rates in trees, but probably the most popular is the heat dissipation method (Granier because of its affordability, relatively low power consumption and ease of use. However, there have been questions about the use of this method in ring-porous species and whether individual species and site calibrations are needed. We made concurrent measurements of sap flow rates using heat dissipation sensors and the heat balance method (Čermák in two oak species (Quercus prinus Willd. and Quercus velutina Lam. and one pine species (Pinus echinata Mill.. We also made concurrent measurements of sap flow rates using both 1-cm long and 2-cm long Granier-style heat dissipation sensors in both oak species. We found that both the heat dissipation and heat balance systems tended to match well in the pine individual, but sap flow rates were underestimated by 2-cm long heat dissipation sensors in five individuals of the two ring-porous oak species. Underestimations of 20-35% occurred in Q. prinus even when a Clearwater correction was applied to account for the shallowness of the sapwood depth relative to the sensor length and flow rates were underestimated by up to 50% in Q. velutina. Two-cm long heat dissipation sensors also underestimated flow rates compared with 1-cm long sensors in Q. prinus, but only at large flow rates. When 2-cm long sensor data in Q. prinus were scaled using the regression with 1-cm long data, daily flow rates matched well with the rates measured by the heat balance system. Daily plot-level transpiration estimated using heat dissipation sap flow rates was about 15% lower than those estimated by the heat balance method at the highest flow rates. Therefore, these results suggest that 1-cm long sensors are appropriate in species with shallow sapwood, however more corrections may be necessary in ring

  10. Horizontal transfer of tet(M) and erm(B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats

    DEFF Research Database (Denmark)

    Jacobsen, Louise; Wilcks, Andrea; Hammer, Karin

    2007-01-01

    Two wild-type strains of Lactobacillus plantarum previously isolated from fermented dry sausages were analysed for their ability to transfer antibiotic resistance plasmids in the gastrointestinal tract. For this purpose, we used gnotobiotic rats as an in vivo model. Rats were initially inoculated......(2) CFU g(-1) faeces towards the end of the experiment. For erm(B)-TCs, the number was significantly higher and increased to c. 10(3) CFU g(-1) faeces. To our knowledge, this is the first study showing in vivo transfer of wild-type antibiotic resistance plasmids from L. plantarum to E. faecalis....

  11. Ratastooli-Phil lõikas ministri jalad ära / Rein Sikk

    Index Scriptorium Estoniae

    Sikk, Rein, 1961-

    2003-01-01

    Sotsiaalminister Marko Pomerantsi ja Phare programmi koostööna läbi viidud üritusest "Puuetega inimeste tööhõive edendamine", kus osales ka inglise puuetega inimeste õiguste eest võitleja Phil Friend

  12. Sievišķā ķermeņa ideāla (de)konstrukcija sieviešu žurnālos 2005. gadā. „Cosmopolitian” un „Santa” piemērs

    OpenAIRE

    Spilberga, Santa

    2006-01-01

    Atslēgas vārdi: sievišķā ķermeņa ideāls; sievišķā ķermeņa reprezentācija populārajā kultūrā; slaiduma un jaunības kults; sievišķā ķermeņa disciplinēšana; izskats un sievišķība; ķermenis patērētājkultūrā; sieviešu žurnālu semiotika. Maģistra darba „Sievišķā ķermeņa ideāla (de)kontrukcija sieviešu žurnālos 2005. gadā. „Cosmopolitan” un „Santa” piemērs.” mērķis ir noskaidrot kādu sievišķā ķermeņa ideālu konstruē šie Latvijā populārākie ikmēneša sieviešu žurnāli, kādi atribūti to veido, kā a...

  13. Characterization of a plasmid carrying cat, ermB and tetS genes in a foodborne Listeria monocytogenes strain and uptake of the plasmid by cariogenic Streptococcus mutans

    DEFF Research Database (Denmark)

    Li, Lili; Olsen, Rikke Heidemann; Shi, Lei

    2016-01-01

    A multi-drug resistant (MDR) Listeria monocytogenes isolate (serotype 1/2c) was recovered from a quick-frozen rice flour product collected from Langfang city in northern China. PCR screening identified the presence of cat, ermB and tetS genes. The plasmid profile of the strain showed the presence...

  14. Effects of chemical anoxia on NHE1, p38 MAPK, p53, Akt and ERM proteins in NIH3T3 fibroblasts: evidence for a role of NHE1 upstream of p38 MAPK

    DEFF Research Database (Denmark)

    Rentsch, M. L.; Hoffmann, E. K.; Pedersen, Stine Helene Falsig

    2006-01-01

    Activation of the plasma membrane Na+/H+ exchanger NHE1 contributes importantly to ischemic/anoxic cell damage, yet the mechanisms involved are unclear. In NIH3T3 cells, PCR studies confirmed the expression of NHE1 and -8, yet not NHE2, -3, and -4. Chemical anoxia (10 mM azide, 10 min) was associ......38 MAPK activation and increased survival after chemical anoxia. Funding: Danish Natural Sciences Research Council (grant 21-01-0507, 21-04-0535)......Activation of the plasma membrane Na+/H+ exchanger NHE1 contributes importantly to ischemic/anoxic cell damage, yet the mechanisms involved are unclear. In NIH3T3 cells, PCR studies confirmed the expression of NHE1 and -8, yet not NHE2, -3, and -4. Chemical anoxia (10 mM azide, 10 min...... phosphorylation. In summary, in NIH3T3 cells, chemical anoxia was associated with decreased pHi, and activation of NHE1, p38 MAPK, and p53, reduced levels of phosphorylated ERM proteins, and reduced survival. Suggesting an important role of NHE1 upstream of p38 MAPK in anoxic cell death, NHE1 inhibition blocked p...

  15. Rationale, Design, and Baseline Characteristics of the EPICOR Asia Study (Long-tErm follow-uP of antithrombotic management patterns In Acute CORonary Syndrome patients in Asia).

    Science.gov (United States)

    Huo, Yong; Lee, Stephen W-L; Sawhney, Jitendra P S; Kim, Hyo-Soo; Krittayaphong, Rungroj; Nhan, Vo T; Alonso-Garcia, Angeles; Han, Ya Ling; Ge, Junbo; Chin, Chee Tang; Ong, Tiong K; Jan, Stephen; Itoh, Yohji; Vega, Ana Maria; Pocock, Stuart

    2015-09-01

    In-hospital and postdischarge mortality for acute coronary syndromes (ACS) vary across Asia and remain generally poorer than globally. The relationship between real-life antithrombotic management patterns (AMPs) and ACS-related outcomes in Asia is unclear. EPICOR Asia (Long-tErm follow-uP of antithrombotic management patterns In acute CORonary syndrome patients in Asia) (NCT01361386) is a prospective, multinational, observational study of patients discharged after hospitalization for an ACS, with 2-year follow-up. The aim is to describe short- and long-term (up to 2 years post-index event) AMPs in patients hospitalized for ACS and to record clinical outcomes, healthcare resource use, and self-reported health status. Pre- and in-hospital management, AMPs, and associated outcomes, with particular focus on ischemic and bleeding events, will be recorded during the 2-year follow up. Between June 2011 and May 2012, 13 005 patients were enrolled. From these, 12 922 patients surviving an ACS (6616 with STEMI, 2570 with NSTEMI, and 3736 with UA) were eligible for inclusion from 219 hospitals across 8 countries and regions in Asia: China (n = 8214), Hong Kong (n = 177), India (n = 2468), Malaysia (n = 100), Singapore (n = 93), South Korea (n = 705), Thailand (n = 957), and Vietnam (n = 208). EPICOR Asia will provide information regarding clinical management and AMPs for ACS patients in Asia. Impact of AMPs on clinical outcomes, healthcare resource use, and self-reported health status both during hospitalization and up to 2 years after discharge will also be described. © 2015 Wiley Periodicals, Inc.

  16. Characterisation of a novel integrative and conjugative element ICESsD9 carrying erm(B) and tet(O) resistance determinants in Streptococcus suis, and the distribution of ICESsD9-like elements in clinical isolates.

    Science.gov (United States)

    Huang, Kaisong; Song, Yajing; Zhang, Qiang; Zhang, Anding; Jin, Meilin

    2016-12-01

    This study identified a novel integrative and conjugative element (ICESsD9) carrying erm(B) and tet(O) resistance determinants in Streptococcus suis D9 and determined its prevalence in clinical isolates. Comparative genome analysis was performed using Mauve and Artemis Comparison Tool visualisation programs. Inverse PCR was utilised to detect its circular intermediate. The transfer capacity of ICESsD9 was evaluated by mating assays using S. suis A7 and Enterococcus faecalis JH2-2 as recipients. A genome walking approach was employed to analyse the characteristics of integration sites in transconjugants. A total of 118 clinical S. suis isolates were tested by PCR mapping assays to detect ICESsD9-like elements. MLST was performed on isolates containing ICESsD9 variants to determine their clonal relatedness. This 55 683-bp element can actively excise from the chromosome. Additionally, it was capable of transferring both into S. suis and E. faecalis with frequencies of 1.2×10(-4) and 5.8×10(-6) per donor, respectively. When investigating integration site features, it was found that ICESsD9 can enter S. suis and E. faecalis chromosomes by different sites, generating 15-bp and 3-bp direct repeat sequences, respectively. Twelve isolates mainly belonging to sequence types ST1, ST7 and ST28 were confirmed to harbour ICESsD9-like elements. In conclusion, this study provides the first description of an ICE in S. suis that is capable of transferring both into S. suis and E. faecalis. The presence of different ICESsD9 variants in clinical isolates suggests already wide dissemination of this family element in S. suis in China. Copyright © 2016 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

  17. What is a good Toothbrush, erm, Password?

    CERN Multimedia

    Computer Security Team

    2011-01-01

    Do you remember the answer to the question posed in the previous issue of the Bulletin? "Your password should be treated like a toothbrush: do not share it and change it regularly!" After our last article, we received a series of questions on how to choose a good password and remember it easily… Thus, here we go.   A good password is: • private: used and known by one person only; • secret: it does not appear in clear text in any file or program or on a piece of paper pinned to the monitor; • easily remembered: so there is no need to write it down; • at least 8 characters long with a mixture of at least 3 of the following: upper case letters, lower case letters, digits and symbols; • not to be found in a dictionary of any major language nor guessable by any program in a reasonable time.   Here are some hints to help you choose good passwords: • Choose a line o...

  18. Factors responsible for subclinical mastitis in cows caused by Staphylococcus chromogenes and its susceptibility to antibiotics based on bap, fnbA, eno, mecA, tetK, and ermA genes.

    Science.gov (United States)

    Bochniarz, M; Adaszek, Ł; Dzięgiel, B; Nowaczek, A; Wawron, W; Dąbrowski, R; Szczubiał, M; Winiarczyk, S

    2016-12-01

    The aim of this study was to recognize selected factors of virulence determining the adhesion of Staphylococcus chromogenes to cows' udder tissues in subclinical mastitis and to evaluate the susceptibility of this pathogen to antibiotics. The subjects of the study were 38 isolates of Staph. chromogenes from 335 samples of milk from cows with subclinical coagulase-negative staphylococci mastitis. Somatic cell count ranged between 216,000 and 568,000/mL of milk (average 356,000/mL of milk). We confirmed the ability to produce slime in 24 isolates (63.2%), and the ability to produce protease in 29 isolates (76.3%). In each slime-producing isolate, the bap gene was not found, and the fnbA and eno genes were not detected. In vitro tests showed that ceftiofur had the highest effectiveness against Staph. chromogenes (89.5% of susceptible isolates). Minimum inhibitory concentrations ranged from 0.06 to 2µg/mL for susceptible isolates. The minimum concentrations required to inhibit growth of 90 and 50% of the isolates for ceftiofur were at or below the cutoffs recommended by the Clinical and Laboratory Standards Institute (2 and 0.06µg/mL, respectively). A significant percentage of the isolates were susceptible to other β-lactam antibiotics: amoxicillin with clavulanic acid (84.2%) and ampicillin (81.6%). The lowest effectiveness among β-lactams was for penicillin (73.7% of susceptible isolates), and the minimum inhibitory concentration for penicillin ranged from <0.06 to 8µg/mL. None of the examined isolates had the mecA gene, but β-lactamase was detected in 4 isolates (10.5%). Erythromycin and oxytetracycline exhibited the lowest activity against Staph. chromogenes (71.1 and 63.2% of susceptible isolates, respectively). The genes tetK (6 isolates) and ermA (1 isolate) were also detected. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  19. Wie das menschliche Gehirn Orientierung ermöglicht

    NARCIS (Netherlands)

    Janzen, G.

    2005-01-01

    People spend a great deal of their time navigating through their environment. To be able to find our way home, we need to store important spatial information in memory. How the brain learns and retrieves the relevance of landmarks at key decision points was so far unknown. With using functional

  20. Ermächtigung zum Hören

    Directory of Open Access Journals (Sweden)

    Wagerer, Wolfgang

    2017-11-01

    Full Text Available To establish a sound basis for an interface that connects biblical studies with the interests of pupils, this paper tries to establish the auditory dimension as a pivotal experience. Such an approach corresponds with the didactic concepts as laid down in the Bible itself as well as with the nature of human beings. In order to enable biblical texts to get a hearing that provides a “moment of freedom” (H. Domin, a re-enactment of these texts in an auditory form, which contains not only the content of the narrative but also the formal elements, structures and principles of composition of the biblical sequence, is mandatory.

  1. 'I'ERM INCUBATION PERIODS IN ASSESSING THE IMMUNO ...

    African Journals Online (AJOL)

    OF I25 I-LAI}ELLED LUTEINIZING HORMONE AND PROLACTIN. Where preparation of sarnples is unnecessary the technique of radioimmunoassay is particulariy convenient to processing large numbrers of samples and several hundred determinations can be set-up in one day. The antiserurn dilution used in the assay is ...

  2. Minimal Substrate Features for Erm Methyltransferases Defined by Using a Combinatorial Oligonucleotide Library

    DEFF Research Database (Denmark)

    Hansen, Lykke H; Lobedanz, Sune; Douthwaite, Stephen

    2011-01-01

    , the main role played by the bottom strand appears to be in maintaining the conformation of the top strand. The addition of multiple LNA residues to the top strand impeded methylation; this indicates that the RNA substrate requires a certain amount of flexibility for accommodation into the active site...

  3. Ole tähelepanelik! / Gabriella Uhl ; tõlkinud Siiri Erm

    Index Scriptorium Estoniae

    Uhl, Gabriella

    2009-01-01

    Eesti graafiku Ülle Marksi ja ungari graafiku Kamilla Sziji näitus Tallinna Ungari Instituudi galeriis 22. maist 16. juunini. Ungari fotograafi Szolt Szamódy fotonäitus Deco galeriis 21.-29. maini

  4. The Netherlands: ERM Comparative Analytical Report on 'Public policy and support for restructuring in SMEs'

    NARCIS (Netherlands)

    van het Kaar, R.H.

    2013-01-01

    Although SMEs get a lot of attention in the Netherlands, there is no specific debate on SMEs and restructuring. A range of public instruments directed at SMEs exists, but their focus is mainly on growth and expansion. Many instruments are fiscal in nature. There are no public or social partner

  5. Persistence of erythromycin resistance gene erm(B) in cattle feedlot pens over time

    Science.gov (United States)

    The prevalence of antibiotic resistance was evaluated in cattle feedlot surface soil samples collected prior to and after a 13-month feedlot trial of two common diets, a 0% wet distillers grains plus solubles (WDGS) corn-based ration versus a 35% WDGS ration. Soil samples were collected from the mo...

  6. Are ERM (ezrin/radixin/moesin) proteins targets for autoantibodies in demyelinating neuropathies?

    Science.gov (United States)

    Miyaji, Kazuki; Shahrizaila, Nortina; Umapathi, Thirugnanam; Chan, Yee-Cheun; Hirata, Koichi; Yuki, Nobuhiro

    2014-11-01

    Ezrin, radixin and moesin, which are strongly expressed in the Schwann cell microvilli, are putative targets for autoantibodies in acute or chronic inflammatory demyelinating polyneuropathy (AIDP or CIDP). An association between anti-moesin IgG antibodies and cytomegalovirus-related AIDP has been postulated. None of 41 AIDP patients, including 8 cytomegalovirus-related AIDP patients, and 23 CIDP had IgG or IgM antibodies to ezrin, radixin and moesin; whereas, one patient with cytomegalovirus-related AIDP had anti-ezrin IgM antibodies. Ezrin, radixin and moesin are unlikely targets for autoantibodies in AIDP and CIDP, and the association of anti-moesin antibodies with cytomegalovirus-related AIDP was not confirmed. Copyright © 2014 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

  7. Eramu interjöör Aaviku elamurajoonis Harjumaal = Interior of a private home, Aaviku / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2005-01-01

    Aaviku asumi tüüpmaja sisekujunduse kontseptsioon on rajatud vineeri erinevatele kasutusviisidele. Vineeriga on kaetud seinad, laed, vineerist on köögilaud ja selle jalad jm. Sisearhitekt Tiiu Truus. 5 värv. ill

  8. Maanoorte elu pole üldsegi nukker / Marit Kannelmäe

    Index Scriptorium Estoniae

    Kannelmäe, Marit

    2008-01-01

    Utrechti Ülikoolis kaitstud magistritööst: "Youth Participation in Rural Areas of Estonia - Equal Opportunities for Rural Youth", millest selgus, et noored, kellele on antud võimalus olla aktiivne ja ennast proovile panna, tunnevad, et nende arvamus läheb korda ning neil on endil üha rohkem algatusvõimet

  9. Russian oil exports : the Baltic Sea remains important route / Kari Liuhto

    Index Scriptorium Estoniae

    Liuhto, Kari

    2003-01-01

    Kui Venemaa jätkab naftatranspordi suurendamist Läänemere sadamate kaudu, siis paneb see EL-i ja Venemaa suhted tõsiselt proovile, leiab autor ning et keskkonnale kõige ohutum viis naftat Venemaalt läände eksportida oleks läbi Murmanski sadama Põhja-Jäämeres. Tabel: Venemaa toornafta eksport 2002. aastal.

  10. Metrology for the radiological early warning system in Europe. The ''MetroERM'' project; Metrologie fuer die radiologischen Fruehwarnsysteme in Europa. Das ''MetroERM''-Projekt

    Energy Technology Data Exchange (ETDEWEB)

    Neumaier, Stefan; Dombrowski, Harald [Physikalisch-Technische Bundesanstalt, Braunschweig (Germany)

    2017-10-01

    In the European radiological early warning system about 5500 stationary sensors are actually implemented that measure continuously the local dose rate. Alongside several hundred air dust collecting systems attribute to the measurement of airborne radioactivity. The local dose rates are transmitted hourly to a central data base that is operated on behalf of the EU commission. In significantly larger time intervals the measured data of the airborne radioactivity are transmitted. In case of a cross-border radiological accident these data are the basis for recommendations given by the EU commission to the member states for national counter measures. The focus of the project ''Metrology for radiological early warning networks in Europe'' is an improvement of the metrological quality and harmonization these data and the development of improved measuring systems and evaluation methods.

  11. CT-guided radiofrequency ablation of T1a renal cell carcinma in Korea; Mid-erm outcomes

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hae Jin; Park, Byung Kwan; Park, Jung Jae; Kim, Chan Kyo [Dept. of Radiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

    2016-09-15

    To evaluate the mid-term outcomes of percutaneous radiofrequency ablation (RFA) treatment in patients with small (< 4 cm) renal cell carcinoma (RCC) in Korea. Between 2010 and 2015, 51 patients (40 men and 11 women; median age, 57 years) with biopsyproven 51 RCC were treated using CT-guided RFA. All patients were clinically staged T1aN0M0 prior to RFA. The median tumor size and follow-up period were 2.1 cm (range, 1.0–3.9 cm) and 26 months (4–60 months), respectively. Local tumor progression, distant metastasis, primary and secondary effectiveness rates, and major complication rates were recorded. Estimated glomerular filtration rates (GFRs) between pre-RFA and last follow-up were compared using paired t tests. The 2-year recurrence-free survival rate was calculated using Kaplan-Meier survival analysis. Of the 51 patients, 2 (3.9%) experienced local tumor progression, and 1 (2.0%) had lymph node metastasis after the first RFA session. Primary and secondary effectiveness rates were 96.1% (49/51) and 100% (1/1), respectively. Only 1 patient experienced a major complication (uretero-pelvic stricture) after the second RFA session for treating a local tumor progression, and the major complication rate was 1.9% (1/52). The median pre-RFA and last follow-up GFRs were 87.1 mL/ min/1.73 m{sup 2} (14.2–142.7 mL/min/1.73 m{sup 2}) and 72.0 mL/min/1.73 m{sup 2} (7.2–112.6 mL/min/1.73 m{sup 2}), respectively (p < 0.0001). The 2-year recurrence-free survival rate was 96.0%. CT-guided RFA is a safe and effective treatment in Korean patients with T1a RCC because of excellent mid-term outcomes.

  12. Long{sub t}erm performance of structural component of intermediate- and low-level radioactive waste disposal facility

    Energy Technology Data Exchange (ETDEWEB)

    Whang, J. H.; Kim, S. S.; Chun, T. H.; Lee, J. M.; Yum, M. O.; Kim, J. H.; Kim, M. S. [Kyunghee Univ., Seoul (Korea, Republic of)

    1997-03-15

    Underground repository for intermediate- and low-level radioactive waste is to be sealed and closed after operation. Structural components, which are generally made of cement concrete, are designed and accommodated in the repository for the purpose of operational convenience and stability after closure. To forecast the change of long-term integrity of the structural components, experimental verification, using in-situ or near in-situ conditions, is necessary. Domestic and foreign requirements with regard to the selection criteria and the performance criteria for structural components in disposal facility were surveyed. Characteristics of various types of cement were studied. Materials and construction methods of structural components similar to those of disposal facility was investigated and test items and methods for integrity of cement concrete were included. Literature survey for domestic groundwater characteristics was performed together with Ca-type bentonite ore which is a potential backfill material. Causes or factors affecting the durability of the cement structures were summarized. Experiments to figure out the ions leaching out from and migrating into cement soaked in distilled water and synthetic groundwater, respectively, were carried out. And finally, diffusion of chloride ion through cement was experimentally measured.

  13. ERM-i uus maja võtab kuju arhitektide ja muuseumi koostöös / Krista Aru

    Index Scriptorium Estoniae

    Aru, Krista, 1958-

    2007-01-01

    Eesti Rahva Muuseumi uue hoone projekteerimislepingu ettevalmistamisest. Läbirääkimiste ajal koostati Tartu Raadi lõunaosa detailplaneering, selgitati maa-ala geoloogilised ja hüdrogeoloogilised tingimused ning arhitektid täiendasid võidutöö "Memory Field" algideed

  14. An Initial Model for Generative Design Research: Bringing Together Generative Focus Group (GFG) and Experience Reflection Modelling (ERM)

    Science.gov (United States)

    Bakirlioglu, Yekta; Ogur, Dilruba; Dogan, Cagla; Turhan, Senem

    2016-01-01

    Understanding people's experiences and the context of use of a product at the earliest stages of the design process has in the last decade become an important aspect of both the design profession and design education. Generative design research helps designers understand user experiences, while also throwing light on their current needs,…

  15. Arhitektuurselt põnev, kuid muuseumina saamatu võidutöö : ERM / Rein Murula ; interv. Reet Varblane

    Index Scriptorium Estoniae

    Murula, Rein, 1955-

    2006-01-01

    Intervjuu Eesti Rahva Muuseumi hoone arhitektuurivõistluse žüriiliikme arhitekt Rein Murulaga. Konkursist, kaasaegsest muuseumihoonest, võidutööst "Memory Field" (autorid itaalia arhitekt Dan Dorell, liibanoni arhitekt Lina Ghotmeh, jaapani arhitekt Tsuyoshi Tane), II preemia saanud tööst "Gems" (autorid soome arhitektid Juho Grönholm, Antti Nousjoki, Janne Teräsvirta, Samuli Woolston), III preemia saanud tööst "Estonia is on the verge of a new millennium" (autorid austria arhitektid Alfred Bramberger, Thomas Pucher, Heidrun Steinhauser, Martin Mathy, Christa Pucher), ostupreemia pälvinud töödest "Crochet" (AB Kosmos: Ott Kadarik, Villem Tomiste, Mihkel Tüür, Krista Saluveer, Urmas Oja) ja "Lake Whisper" (autorid šveitsi arhitektid Manuel Bieler, Marco de Francesco, Antoine Robert-Grandpierre, Laurent Saurer)

  16. Vaccine Induced Specific Protection Against Enteric Red Mouth Disease (ERM) Caused by Yersinia Ruckeri Serotype 1 Biotype 2

    DEFF Research Database (Denmark)

    Deshmukh, Sidharta; Raida, Martin K.; Dalsgaard, Inger

    2011-01-01

    biotype 1 and 2. In this study, the specificity of immune protection extended by three commercial vaccines viz; AQUAVAC ERM® Intervet Schering Plough (based on biotype 1 only), ERMOGEN VET® Novartis (based on biotype 1 only) and AQUAVAC RELERA® Intervet Schering Plough (based on both biotype 1and 2...... by AQUAVAC RELERA® in terms of milder and lesser degree of certain pathological lesions like haemorrhages in or around the buccal cavity, base of fins and intestines, when compared to ERMOGEN VET®, AQUAVAC ERM® vaccinated group and Yersinia ruckeri (BT2) infected group....

  17. Níveis de triglicerídeos intra e extracelulares em músculos humanos mediante ¹H-ERM: um estudo de caso Niveles de triglicéridos intra y extracelulares en músculos humanos mediante 1H-ERM: estudio de caso Levels of intra and extra cellular triglycerides in human muscles by means of ¹H-ERM: a case study

    Directory of Open Access Journals (Sweden)

    Maria Gisele dos Santos

    2004-10-01

    Full Text Available O objetivo deste estudo foi analisar o consumo de triglicerídeos intra (IT e extracelulares (ET nos músculos sóleo, tibial anterior e vasto medial após uma prova de quatro horas de ciclismo de estrada. Esta pesquisa caracterizou-se por ser um estudo de caso de um ciclista que participa de competições internacionais. Os estudos de ressonância magnética utilizaram os seguintes parâmetros espectrais impostos para o ajuste no domínio do tempo, como a distância das freqüências entre os sinais de IT e ET. Os valores de amplitudes dos triglicerídeos intra e extracelulares foram divididos pela ressonância de água. Concluímos que o músculo vasto medial do ciclista apresentou maior consumo de triglicerídeos depois de quatro horas de ciclismo em estrada. Portanto, constatou-se que um trabalho com intensidade de 80% da freqüência cardíaca máxima permitiu consumo de triglicerídeos intramusculares durante o exercício.El objetivo de este estudio fue el de analizar el consumo de triglicéridos intra (IT y extracelulares (ET en los músculos soleo, tibial anterior y vasto medial tras una prueba de cuatro horas de ciclismo de ruta. Esta investigación es un estudio de caso de un ciclista que participa de competiciones internacionales. Los estudios de resonancia magnética utilizaron los siguientes parámetros espectrales que se impusieron para el ajuste en el dominio del tiempo, como la distancia de las frecuencias entre las señales de IT e ET. Los valores de amplitudes de los triglicéridos intra y extracelulares fueron divididos por resonancia de agua. Concluimos que el músculo vasto medial del ciclista presentó mayor consumo de triglicéridos luego de cuatro horas de ciclismo en ruta. Por lo tanto, se verificó que un trabajo con intensidad del 80% de la frecuencia cardiaca máxima permitió el consumo de triglicéridos intramusculares durante el ejercicio.The objective of this study was to analyze the intra (IT and extra cellular (ET triglycerides consumption in the soleus, tibialis anticus, vastus internus muscles, after 4 hours training ride on the road. This study used a top-level cyclist as a case study. Magnetic resonance studies used the following spectroscopy parameters imposed to adjust the time, such as the distance of the frequency between the signs IT and ET of the similar chemical nature. We were able to conclude that the vastus internus was the muscle which demonstrated the largest triglyceride consumption as the energetic substrate after a 4 hour bike ride on the road. Thus, we can infer that a workout at 80% of the VO2max enabled the consumption of intra muscle triglyceride during exercise.

  18. Data Records derived from GEOSAT Geodetic Mission (GM) and Exact Repeat Mission (ERM) data from 30 March 1985 to 31 December 1989

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This collection contains Sensor Data Records (SDRs), Geodetic Data Records (GDRs), Waveform Data Records (WDRs), and Crossover Difference data Records (XDRs) from...

  19. Kutsume kaasa mõtlema ka neid, keda liikluskultuur külmaks jätab! / Aire Tammik, Veronika Ruut, Lii Erm

    Index Scriptorium Estoniae

    Tammik, Aire

    2011-01-01

    TLÜ Haapsalu kolledži liiklusohutuse üliõpilaste artikkel kutsub üles kaasa mõtlema liikluskultuuri teemadel ning vaatama TV3-s esilinastuvat Märt Treieri dokumentaalfilmi "Georg", mis vaatleb ühe õnnetuse eel- ja järellugu, kuritegu ja karistust

  20. Orienteerujad vallutavad kõrgusi olümpiaadimaastikul / Oleg Košik ; kommenteerinud Tõnis Erm, Riho Taba, Arvo Kivikas

    Index Scriptorium Estoniae

    Košik, Oleg, 1985-

    2010-01-01

    Aastatel 1998-2010 Euroopa noorte meistrivõistlustel ning juunioride maailmameistrivõistlustel Eesti võistkonda kuulunud orienteerujatest, kellel on ettenäidata esinemine vähemalt Eesti olümpiaadi lõppvoorus reaal- või loodusaines

  1. Increasing Range and Lethality of Extended-Range Munitions (ERMS) using Numerical Weather Prediction (NWP) and the AUV Workbench to Compute a Ballistic Correction (BALCOR)

    Science.gov (United States)

    2006-12-01

    DMSP) polar- orbiting satellites, and DMSP Special Sensor Microwave Imager (SSM/I) data. This satellite data provides surface wind speed...of Employment (2008-2018). Arlington, VA: EDO Professional Services. McGee, T. (2006). Battlespace on Demand: Commander’s Intent Meteorology...Center, WV 26. Scott Davis ATK Advanced Weapons Division Woodland Hills, CA 27. Curtis Marsh EDO Professional Services Arlington, VA 28

  2. Níveis de triglicerídeos intra e extracelulares em músculos humanos mediante ¹H-ERM: um estudo de caso

    OpenAIRE

    Santos,Maria Gisele dos; ladewig,Iverson; Osiecki,Raul; Gomes,André; Calvar,Jorge Andrés

    2004-01-01

    O objetivo deste estudo foi analisar o consumo de triglicerídeos intra (IT) e extracelulares (ET) nos músculos sóleo, tibial anterior e vasto medial após uma prova de quatro horas de ciclismo de estrada. Esta pesquisa caracterizou-se por ser um estudo de caso de um ciclista que participa de competições internacionais. Os estudos de ressonância magnética utilizaram os seguintes parâmetros espectrais impostos para o ajuste no domínio do tempo, como a distância das freqüências entre os sinais de...

  3. Efeitos dentais e esqueletais mediatos da E.R.M. utilizando o disjuntor Hyrax RME dental and skeletal effects with Hyrax appliance

    Directory of Open Access Journals (Sweden)

    Carla Mauad P. Ferreira

    2007-01-01

    Full Text Available OBJETIVO: o aparelho disjuntor tipo Hyrax tem sido apontado como o aparelho expansor da maxila que melhor permite ao paciente efetuar sua higienização. Porém, por não ser dento-muco-suportado e por não possuir nenhum dispositivo de controle vertical, presume-se que o aparelho Hyrax causaria, em amplitude maior que os disjuntores tipo Haas e de cobertura oclusal, inclinações vestibulares dos dentes de apoio, agravando os efeitos colaterais no sentido vertical. Por isso, esse tipo de aparelho de disjunção maxilar seria contra-indicado para pacientes esqueleticamente divergentes. Sendo assim, o presente estudo foi realizado com o intuito de verificar se as alterações pós-disjunção, já amplamente estudadas, persistem ou se, em pacientes que se encontram em fase de crescimento e em plenas condições de adaptações musculares, tais alterações seriam dissipadas com o tempo. METODOLOGIA: para isso, foram avaliadas radiografias cefalométricas em norma lateral, tomadas pré-disjunção e pós-disjunção (em média de 2 anos e 9 meses pós-disjunção de 30 pacientes com faixa etária de 7 anos e 8 meses, em média, no T1 (inicial e 10 anos e 7 meses no T2 (final. Tais pacientes apresentavam dentadura mista e necessidade de ganho transversal da maxila e, por isso, foram submetidos à expansão maxilar com aparelho do tipo Hyrax até que se conseguisse a sobrecorreção. Este grupo, tratado apenas com disjunção, foi comparado com um outro grupo controle de 30 pacientes com faixa etária média de 9 anos e 4 meses no NT1 (inicial e 12 anos e 3 meses no NT2 (final, dos quais foram feitas tomadas radiográficas iniciais e finais (após, em média, 2 anos e 9 meses; esses pacientes não sofreram nenhum tipo de tratamento ortodôntico. RESULTADOS E CONCLUSÃO: estatisticamente, quando foram comparados através de grandezas cefalométricas, os dois grupos não apresentaram diferenças significativas, tanto na fase inicial quanto após o período de tratamento/acompanhamento. Assim, os resultados indicaram que, em médio prazo, os possíveis efeitos dento-esqueléticos indesejáveis da disjunção maxilar com o aparelho Hyrax desaparecem, provavelmente, compensados com o crescimento, a atividade muscular e a oclusão.AIM: The Hyrax appliance has been considered the easiest to clean maxillary expander. However, it is presumed to present the most significant dentoalveolar effects due to not present any tissue bearing contact. Is has been speculated that it would produce more buccal inclination of the posterior teeth as well as more extrusion. Therefore, it would not be indicated in patients with vertical growth patterns. This study was conducted to verify if any harmful short term effects of rapid maxillary expansion (RME remain over time in growing patients. METHODS: Lateral cephalometric radiographs were taken before and after RME (average 2 years and 9 months in 30 patients with an initial mean age of 7 years and 8 months and a final mean age of 10 years and 7 months. All patients were initially in the mixed dentition and presented clinical signs of maxillary constriction. They were subjected to maxillary expansion with a Hyrax appliance until overcorrection was obtained. This group was compared to a untreated control group comprising 30 patients with a initial mean age of 9 years and 9 months and a final age of 12 years and 3 months, with a mean observation period of 2 years and 9 months. RESULTS AND CONCLUSIONS: There were statistically significant differences between the two groups, both at initial and at the final observation. It was concluded that any adverse effects produced by the Hyrax appliance at the early stages of therapy do not remain over time, probably compensated by growth, muscular activity and occlusion.

  4. Klientide ees põlvitamise kunst / Sam Swaminathan

    Index Scriptorium Estoniae

    Swaminathan, Sam

    2003-01-01

    Autor selgitab erinevate ettevõtete kogemuste varal kliendikeskse teeninduse põhimõtteid ning rõhutab, et kõikide klientidega tuleb käituda erinevalt. Kokkuvõte autori ettekandest turunduskonverentsil "Jalad maas" Pärnus 22.-23. mail 2003. Vt. samas: Kuidas kliendikesksuse teel esimene samm teha?

  5. Improviseeritud lugu ja zhanriga mäng / Donald Tomberg

    Index Scriptorium Estoniae

    Tomberg, Donald

    2003-01-01

    Eesti komöödiafilm "Vanad ja kobedad saavad jalad alla" : režissöör Rando Pettai : operaator Mait Mäekivi : näitlejad Henrik Normann, Madis Milling, Robert Gutman jt. : muusika - ansambel Genialistid : Ruut Pictures 2003

  6. Seltskond / Pepe Le Punk

    Index Scriptorium Estoniae

    Pepe Le Punk

    2003-01-01

    Uue eesti komöödiafilmi "Vanad ja kobedad saavad jalad alla" esilinastuse järgsel afterparty'l küsiti filmiga seotud inimestelt "Mis teid filmis kõige rohkem naerutas?". Küsitlus on ajalehe numbriteta värvilistel sisekülgedel

  7. Käiguvahetuse kasulikkusest / Herbert Vainu

    Index Scriptorium Estoniae

    Vainu, Herbert, 1929-2011

    2004-01-01

    Autori arvates saavutas Eesti alguses küll kiire majandustõusu, kuid nüüd hakkab avalduma teistest endistest sotsialismimaadest ettejõudmise pahupool. Tema sõnul oleme ise löönud jalad alt edasiselt kiirelt majandusarengult

  8. Ameeriklased arvavad / Jaan Ruus

    Index Scriptorium Estoniae

    Ruus, Jaan, 1938-2017

    2004-01-01

    Refereeritakse välismaal ilmunud arvustusi eesti komöödiafilmi "Vanad ja kobedad saavad jalad alla" : Leslie Felperini arvustus Ameerika filmitööstuse ajakirjas "Variety" (15.12.2003) ja Harper Barnesi oma USA Missouri osariigi St. Louisi ajalehes "St. Louis Post Dispatch"

  9. Venemaa õppetund / Silver Meikar

    Index Scriptorium Estoniae

    Meikar, Silver, 1978-

    2008-01-01

    Ilmunud ka: Vooremaa 19. aug. lk. 2, Lõunaleht 28. aug. lk. 4. Eesti-Gruusia parlamendirühma aseesimehe sõnul peab Eesti jätkama toetust Gruusiale selle nimel, et sõjas laastatud riik saaks jalad alla ning selle kodanikud võiksid elada vabas ja demokraatlikus riigis

  10. Naise purunematu ilu / Triin Toome

    Index Scriptorium Estoniae

    Toome, Triin

    2005-01-01

    Fotograaf Laura Kallasvee ja grimmikunstnike kolmiku Grimmsisters (Helen Ehandi, Kaire Hendrikson ja Maarja Sild) ühisnäitusest "She has really long legs" ("Tal on väga pikad jalad") kohvik Moskvas, Tallinnas. Näitus koosneb tuntud naiste portreedest

  11. Hakkame alasti laudadel tantsima : Mõtteid uuemast eesti filmikunstist / Kalle Käsper

    Index Scriptorium Estoniae

    Käsper, Kalle, 1952-

    2003-01-01

    Autor arvustab viimaste aastate mängufilme : "Somnambuul", "Agent Sinikael", "Nimed marmortahvlil"(1. artiklis), "Karu süda", "Kass kukub käppadele", "Aida", "Vanad ja kobedad saavad jalad alla", "Head käed" (2. artiklis). Järgneb 21. nov. 2003, lk. 16 ja 28. nov. 2003, lk. 5

  12. "Vanade ja kobedate" Väino uuendas tutvust maaga / Gert Kiiler

    Index Scriptorium Estoniae

    Kiiler, Gert

    2002-01-01

    RUUT Pictures alustas täispika rahvakomöödia "Vanad ja kobedad saavad jalad alla" või "Seiklusjutte maalt" võtteid Viljandimaal Mustlas, peaosades Henrik Normann ja Madis Milling. Režissöör on Rando Pettai

  13. Eesti tuntumad filmiautod : [Eesti filmiajaloo kõige tuntumad autod – Maanteemuhust rullnoka-BMW-ni

    Index Scriptorium Estoniae

    2009-01-01

    Kuulsaimad autofilmid: 1967 "Keskpäevane praam" - invaliidiauto SMZ S-3-A ; 1979 "Siin me oleme"- ZAZ 965A "Maanteemuhk" ; 1982 "Teisikud" - VAZ 2105 ; 1986 "Keskea rõõmud" - ZAZ 1102 Tauria ; 2000 "Vanad ja kobedad saavad jalad alla"- GAZ 21 Volga ; 2006 "Tulnukas ehk Valdise päästmine 11 osas"- VAZ 2109 Samara ; 2006 "Borat - kultuurialased õppetunnid Ameerikast" - ZAZ 968M

  14. "Me kohal on sinine taevas ..." = "Nebo sineje nad nami ..." : [luuletused] / Anne Lasikov ; tlk. Gennadi Krasnikov

    Index Scriptorium Estoniae

    Lasikov, Anne

    2003-01-01

    Sisu: "Me kohal on sinine taevas ..." = "Nebo sinee nad nami ..." ; "Siin valsirütme keerutasid jalad..." = "Kogda-to ritmõ valsa zdes kruzhili nogi ..." ; Sügisetuju = Osenneje nastroenije ; Ma tahan tagasi ; Kevad ; Suve algus ; Ood Narva-Jõesuule ; "V golovu lezjot vsjakaja drjan..." ; "Kogda vsbessilas avtorutshka..." ; "Ja ukrala tvoi vzgljad..." ; Zatshem mne radost tehh notshei? ; Vzgljad ; Tshernushka o sudbe ; "Zatshem võ, tshaiki, tak kritshite... " ; Nasledstvo ; Ja skisla.

  15. The adoption and design of enterprise risk management practices : An empirical study

    NARCIS (Netherlands)

    Paape, L.; Speklé, R.F.

    2010-01-01

    Using data from 825 organizations, we examine (1) the extent of ERM implementation and the factors that are associated with cross-sectional differences in the level of ERM adoption, and (2) specific ERM design choices and their effect on perceived ERM effectiveness. Broadly consistent with previous

  16. Vliv výkonnostní úrovně šermířů na reakční dobu a aktivaci vybraných svalů při výpadu

    OpenAIRE

    Balkó, Štefan

    2015-01-01

    Title Influence of a performance level of fencers on reaction time and activation of selected muscles during a lunge. Problem The motion efficiency of offensive or defensive actions implemented during the match of fencing is related to the consolidation of movement patterns in the CNS through the training proces and competition situations. In this regard, muscle activation during a lunge, which is the most common form of attack, may be important for the resulting performance of fencers. The a...

  17. Short outlines of books by Estonian authors / Rutt Hinrikus, Janika Kronberg

    Index Scriptorium Estoniae

    Hinrikus, Rutt, 1946-

    2000-01-01

    Andres Ehin. Alateadvus on alatasa purjus; Peeter Torop. Kultuurimärgid; Matt Barker. Sarah' jalad; Jaan Isotamm. Mina Johnny B.; Jaan Kaplinski. Silm. Hektor; Jaan Kaplinski. Kevad kahel rannikul ehk tundeline teekond Ameerikasse; Rein Raud. Pisiasjad, mis omavad tähtsust; Kerttu Rakke. Kalevipoeg; Elin Toona. Rõõm teeb taeva taga tuld; Veera Saar. Neid ammuseid aegu; Toomas Vint. Nädalavahetusel. Mängides; Varjatud ilus haigus : valik sajandilõpu eesti luuletajaid / koostanud Kajar Pruul; Viiu Härm. Duubel kaks

  18. Streptocollin, a type IV lanthipeptide produced by Streptomyces collinus Tü 365

    DEFF Research Database (Denmark)

    Iftime, Dumitrita; Jasyk, Martin; Kulik, Andreas

    2015-01-01

    Lanthipeptides are ribosomally synthesized and posttranslationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of the class IV lanthipeptides. Insertion of the constitutive ermE* promoter...

  19. The Expression of Antibiotic Resistance Methyltransferase Correlates with mRNA Stability Independently of Ribosome Stalling.

    Science.gov (United States)

    Dzyubak, Ekaterina; Yap, M N

    2016-12-01

    Members of the Erm methyltransferase family modify 23S rRNA of the bacterial ribosome and render cross-resistance to macrolides and multiple distantly related antibiotics. Previous studies have shown that the expression of erm is activated when a macrolide-bound ribosome stalls the translation of the leader peptide preceding the cotranscribed erm Ribosome stalling is thought to destabilize the inhibitory stem-loop mRNA structure and exposes the erm Shine-Dalgarno (SD) sequence for translational initiation. Paradoxically, mutations that abolish ribosome stalling are routinely found in hyper-resistant clinical isolates; however, the significance of the stalling-dead leader sequence is largely unknown. Here, we show that nonsense mutations in the Staphylococcus aureus ErmB leader peptide (ErmBL) lead to high basal and induced expression of downstream ErmB in the absence or presence of macrolide concomitantly with elevated ribosome methylation and resistance. The overexpression of ErmB is associated with the reduced turnover of the ermBL-ermB transcript, and the macrolide appears to mitigate mRNA cleavage at a site immediately downstream of the ermBL SD sequence. The stabilizing effect of antibiotics on mRNA is not limited to ermBL-ermB; cationic antibiotics representing a ribosome-stalling inducer and a noninducer increase the half-life of specific transcripts. These data unveil a new layer of ermB regulation and imply that ErmBL translation or ribosome stalling serves as a "tuner" to suppress aberrant production of ErmB because methylated ribosome may impose a fitness cost on the bacterium as a result of misregulated translation. Copyright © 2016 Dzyubak and Yap.

  20. Charting a Course through CORAL: Texas A&M University Libraries' Experience Implementing an Open-Source Electronic Resources Management System

    Science.gov (United States)

    Hartnett, Eric; Beh, Eugenia; Resnick, Taryn; Ugaz, Ana; Tabacaru, Simona

    2013-01-01

    In 2010, after two previous unsuccessful attempts at electronic resources management system (ERMS) implementation, Texas A&M University (TAMU) Libraries set out once again to find an ERMS that would fit its needs. After surveying the field, TAMU Libraries selected the University of Notre Dame Hesburgh Libraries-developed, open-source ERMS,…

  1. Trends in antimicrobial susceptibility in relation to antimicrobial usage and presence of resistance genes in Staphylococcus hyicus isolated from exudative epidermitis in pigs

    DEFF Research Database (Denmark)

    Aarestrup, Frank Møller; Jensen, Lars Bogø

    2002-01-01

    From 1996 to 2001 a total of 467 Staphylococcus hyicus isolates from exudative epidermitis (EE) in pigs in Denmark were examined for susceptibility to 13 different antimicrobial agents. The presence of selected genes encoding macrolide (erm(A), erm(B) and erm(C)), penicillin (blaZ), streptogramin...

  2. A library of 7TM receptor C-terminal tails - Interactions with the proposed post-endocytic sorting proteins ERM-binding phosphoprotein 50 (EBP50), N-ethylmaleimide-sensitive factor (NSF), sorting nexin 1 (SNX1), and G protein-coupled receptor-associated sorting protein (GASP)

    DEFF Research Database (Denmark)

    Heydorn, A.; Sondergaard, B.P.; Ersbøll, Bjarne Kjær

    2004-01-01

    sequestration through interactions, mainly with the C-terminal intracellular tails of the receptors. A library of tails from 59 representative members of the super family of seven-transmembrane receptors was probed as glutathione S-transferase fusion proteins for interactions with four different adaptor...... only a single receptor tail, i.e. the beta(2)-adrenergic receptor, whereas N-ethylmaleimide-sensitive factor bound 11 of the tail-fusion proteins. Of the two proteins proposed to target receptors for lysosomal degradation, sorting nexin 1 (SNX1) bound 10 and the C-terminal domain of G protein......-coupled receptor-associated sorting protein bound 23 of the 59 tail proteins. Surface plasmon resonance analysis of the binding kinetics of selected hits from the glutathione S-transferase pull-down experiments, i.e. the tails of the virally encoded receptor US28 and the delta-opioid receptor, confirmed...

  3. A library of 7TM receptor C-terminal tails. Interactions with the proposed post-endocytic sorting proteins ERM-binding phosphoprotein 50 (EBP50), N-ethylmaleimide-sensitive factor (NSF), sorting nexin 1 (SNX1), and G protein-coupled receptor-associated sorting protein (GASP)

    DEFF Research Database (Denmark)

    Heydorn, Arne; Søndergaard, Birgitte P; Ersbøll, Bjarne

    2004-01-01

    Adaptor and scaffolding proteins determine the cellular targeting, the spatial, and thereby the functional association of G protein-coupled seven-transmembrane receptors with co-receptors, transducers, and downstream effectors and the adaptors determine post-signaling events such as receptor...... sequestration through interactions, mainly with the C-terminal intracellular tails of the receptors. A library of tails from 59 representative members of the super family of seven-transmembrane receptors was probed as glutathione S-transferase fusion proteins for interactions with four different adaptor...... that the tail library provides useful information on the general importance of certain adaptor proteins, for example, in this case, ruling out EBP50 as being a broad spectrum-recycling adaptor....

  4. Spectral domain optical coherence tomography characterization of pediatric epiretinal membranes.

    Science.gov (United States)

    Rothman, Adam L; Folgar, Francisco A; Tong, Amy Y; Toth, Cynthia A

    2014-07-01

    To compare the macular morphology of pediatric versus adult eyes with epiretinal membrane (ERM) using spectral domain optical coherence tomography and identify characteristics associated with postoperative visual acuity. This retrospective study analyzed spectral domain optical coherence tomography from pediatric subjects and a randomly selected cohort of adult subjects with ERM. Morphologic retinal and ERM features were graded by two masked spectral domain optical coherence tomography readers and compared with a postoperative change in visual acuity. Pediatric ERMs (age, 0.3-16.5 years) were more confluently attached to the retina than adult ERMs (age, 40-88 years; P = 0.009) and had less fibrillary appearance of the inner retina when separation was present (P = 0.044). Pediatric ERMs were associated with more vessel dragging (P = 0.019) and less external limiting membrane (P = 0.001) and inner segment band visibility (P = 0.010), with a trend toward foveal sparing by ERM (P = 0.051) and "taco" retinal folds (P = 0.052) compared with adult eyes. Visual acuity improvement was associated with intact (P = 0.048) and smooth (P = 0.055, trend) inner segment band in children and with smooth inner segment band (P = 0.083, trend) and visible external limiting membrane (P = 0.098, trend) in adults. We identified morphologic differences between pediatric and adult ERM on spectral domain optical coherence tomography. Similar to adults, photoreceptor integrity with pediatric ERM seems to predict better visual acuity changes after surgical ERM removal.

  5. Trends in antimicrobial susceptibility and presence of resistance genes in Staphylococcus hyicus isolated from exudative epidermitis in pigs

    DEFF Research Database (Denmark)

    Aarestrup, Frank Møller; Jensen, L. B.

    2002-01-01

    (vat, vga, vga(B), vat(B), vat(D) and vat(E)), streptomycin (aadE) and tetracycline resistance (tet(K), tet(L), tet(M) and tet(O)) were determined in selected isolates. The occurrence of erythromycin resistance increased from 33% in 1996 to a maximum of 62% in 1997 and decreased to 26% in 2001...... streptogramin resistance gene in four streptogramin resistant isolates. Of the 55 erythromycin resistant isolates examined, five contained erm(A), 13 erm(B), 35 erm(C) and two both erm(A) and erm(C). The presence of erm(B) was confirmed by hybridization to plasmid profiles in all 13 PCR-positive isolates. Of 52...

  6. Resistance to antimicrobials and acid and bile tolerance of Bacillus spp isolated from Bikalga, fermented seeds of Hibiscus sabdariffa

    DEFF Research Database (Denmark)

    Compaore, Clarisse S.; Jensen, Lars Bogø; Diawara, Brehima

    2013-01-01

    , streptomycin and trimethoprim) in this study may be intrinsic as no positive amplicon was observed for the most prevalent resistance genes investigated (catpIP501, erm(A), erm(B), erm(C), aph(3”)-I, aph(3”)-III, ant(2”)-I, blaZ, aadA, aadE, StrA, StrB, dfr(A)). Furthermore, based on their good survival in pH 2...

  7. Issues with enterprise risk management buy-in: a South African government case study

    OpenAIRE

    Pillay, Vaneshree Mogeshree

    2015-01-01

    The South African national government implemented enterprise risk management (ERM) to facilitate a strong public sector, able to contribute to economic development and social upliftment across South Africa. ERM principles can improve organisational performance by maximising opportunities to achieve strategic objectives, and minimising the risks in achieving them. This article reports on an exploratory study to identify issues impeding ERM buy-in in a South African government organisation, usi...

  8. Paternal uniparental disomy with segmental loss of heterozygosity of chromosome 11 are hallmark characteristics of syndromic and sporadic embryonal rhabdomyosarcoma.

    Science.gov (United States)

    Robbins, Katherine M; Stabley, Deborah L; Holbrook, Jennifer; Sahraoui, Rebecca; Sadreameli, Alexa; Conard, Katrina; Baker, Laura; Gripp, Karen W; Sol-Church, Katia

    2016-12-01

    Costello syndrome (CS) arises from a typically paternally derived germline mutation in the proto-oncogene HRAS, and is considered a rasopathy. CS results in failure-to-thrive, intellectual disabilities, short stature, coarse facial features, skeletal abnormalities, congenital heart disease, and a predisposition for cancer, most commonly embryonal rhabdomyosarcoma (ERMS). The goal of this study was to characterize CS ERMS at the molecular level and to determine how divergent it is from sporadic ERMS. We characterized eleven ERMS tumors from eight unrelated CS patients, carrying paternally derived HRAS c.34G>A (p.Gly12Ser; 6) or c.35G>C (p.Gly12Ala; 2) mutations. Loss of heterozygosity (LOH) was evaluated in all CS ERMS by microarray and/or short tandem repeat (STR) markers spanning the entire chromosome 11. Eight CS ERMS tumors displayed complete paternal uniparental disomy of chromosome 11 (pUPD11), whereas two displayed UPD only at 11p and a second primary ERMS tumor showed UPD limited to 11p15.5, the classical hallmark for ERMS. Three sporadic ERMS cell lines (RD, Rh36, Rh18) and eight formalin fixed paraffin embedded (FFPE) ERMS tumors were also analyzed for RAS mutations and LOH status. We found a higher than anticipated frequency of RAS mutations (HRAS or NRAS; 50%) in sporadic ERMS cell lines/tumors. Unexpectedly, complete uniparental disomy (UPD11) was observed in five specimens, while the other six showed LOH extending across the p and q arms of chromosome 11. In this study, we are able to clearly demonstrate complete UPD11 in both syndromic and sporadic ERMS. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  9. Quiescent epithelial cell rests of Malassez can differentiate into ameloblast-like cells.

    Science.gov (United States)

    Shinmura, Yuka; Tsuchiya, Shuhei; Hata, Ken-Ichiro; Honda, Masaki J

    2008-12-01

    Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants of Hertwig's epithelial root sheath (HERS) that are involved in the formation of tooth roots. After completion of crown formation, HERS are converted from cervical loop cells, which have the potential to generate enamel for tooth crown formation. Cervical loop cells have the potential to differentiate into ameloblasts. Generally, no new ameloblasts can be generated from HERS, however this study demonstrated that subcultured ERM can differentiate into ameloblast-like cells and generate enamel-like tissues in combination with dental pulp cells at the crown formation stage. Porcine ERM were obtained from periodontal ligament tissue by explant culture and were subcultured with non-serum medium. Thereafter, subcultured ERM were expanded on 3T3-J2 feeder cell layers until the tenth passage. The in vitro mRNA expression pattern of the subcultured ERM after four passages was found to be different from that of enamel organ epithelial cells and oral gingival epithelial cells after the fourth passage using the same expansion technique. When subcultured ERM were combined with subcultured dental pulp cells, ERM expressed cytokeratin14 and amelogenin proteins in vitro. In addition, subcultured ERM combined with primary dental pulp cells seeded onto scaffolds showed enamel-like tissues at 8 weeks post-transplantation. Moreover, positive staining for amelogenin was observed in the enamel-like tissues, indicating the presence of well-developed ameloblasts in the implants. These results suggest that ERM can differentiate into ameloblast-like cells.

  10. Seasonal variation and removal efficiency of antibiotic resistance genes during wastewater treatment of swine farms.

    Science.gov (United States)

    Sui, Qianwen; Zhang, Junya; Tong, Juan; Chen, Meixue; Wei, Yuansong

    2017-04-01

    The seasonal variation and removal efficiency of antibiotic resistance genes (ARGs), including tetracycline resistance genes (tetG, tetM, and tetX) and macrolide (ermB, ermF, ereA, and mefA), were investigated in two typical swine wastewater treatment systems in both winter and summer. ARGs, class 1 integron gene, and 16S rRNA gene were quantified using real-time polymerase chain reaction assays. There was a 0.31-3.52 log variation in ARGs in raw swine wastewater, and the abundance of ARGs in winter was higher than in summer. tetM, tetX, ermB, ermF, and mefA were highly abundant. The abundance of ARGs was effectively reduced by most individual treatment process and the removal efficiencies of ARGs were higher in winter than in summer. However, when examining relative abundance, the fate of ARGs was quite variable. Anaerobic digestion reduced the relative abundance of tetX, ermB, ermF, and mefA, while lagoon treatment decreased tetM, ermB, ermF, and mefA. Sequencing batch reactor (SBR) decreased tetM, ermB, and ermF, but biofilters and wetlands did not display consistent removal efficiency on ARGs in two sampling seasons. As far as the entire treatment system is concerned, ermB and mefA were effectively reduced in both winter and summer in both total and relative abundance. The relative abundances of tetG and ereA were significantly correlated with intI1 (p wastewater treatment. This may pose a great threat to public health.

  11. Ezrin/radixin/moesin proteins differentially regulate endothelial hyperpermeability after thrombin

    Science.gov (United States)

    Dudek, Steven M.; Moldobaeva, Nurgul; Kim, Kyung-mi; Ma, Shwu-Fan; Kasa, Anita; Garcia, Joe G. N.; Verin, Alexander D.

    2013-01-01

    Endothelial cell (EC) barrier disruption induced by inflammatory agonists such as thrombin leads to potentially lethal physiological dysfunction such as alveolar flooding, hypoxemia, and pulmonary edema. Thrombin stimulates paracellular gap and F-actin stress fiber formation, triggers actomyosin contraction, and alters EC permeability through multiple mechanisms that include protein kinase C (PKC) activation. We previously have shown that the ezrin, radixin, and moesin (ERM) actin-binding proteins differentially participate in sphingosine-1 phosphate-induced EC barrier enhancement. Phosphorylation of a conserved threonine residue in the COOH-terminus of ERM proteins causes conformational changes in ERM to unmask binding sites and is considered a hallmark of ERM activation. In the present study we test the hypothesis that ERM proteins are phosphorylated on this critical threonine residue by thrombin-induced signaling events and explore the role of the ERM family in modulating thrombin-induced cytoskeletal rearrangement and EC barrier function. Thrombin promotes ERM phosphorylation at this threonine residue (ezrin Thr567, radixin Thr564, moesin Thr558) in a PKC-dependent fashion and induces translocation of phosphorylated ERM to the EC periphery. Thrombin-induced ERM threonine phosphorylation is likely synergistically mediated by protease-activated receptors PAR1 and PAR2. Using the siRNA approach, depletion of either moesin alone or of all three ERM proteins significantly attenuates thrombin-induced increase in EC barrier permeability (transendothelial electrical resistance), cytoskeletal rearrangements, paracellular gap formation, and accumulation of phospho-myosin light chain. In contrast, radixin depletion exerts opposing effects on these indexes. These data suggest that ERM proteins play important differential roles in the thrombin-induced modulation of EC permeability, with moesin promoting barrier dysfunction and radixin opposing it. PMID:23729486

  12. Molecular Detection of Inducible Clindamycin Resistance among Staphylococcal Strains Isolated from Hospital Patients

    Directory of Open Access Journals (Sweden)

    Shadiyeh Abdollahi

    2013-04-01

    Full Text Available Background & Objectives: Macrolide, lincosamide and streptogramin B (MLSB antimicrobial agents are used in the treatment of staphylococcal infections. They prevent the microbial protein synthesis system through binding to 23 S rRNA. The aim of this study was to apply molecular methods to detect inducible clindamycin resistance genes among staphylococcal strains isolated from clinical specimens.   Methods : Two hundred staphylococcus strains were isolated from nose and throat swabs of patients in Toohid and Besat hospitals in Sanandaj . Antimicrobial susceptibilities of isolates were determined using disc diffusion method, agar screen test and D-Test. A multiplex PCR was performed using primers specific for erm (A, B, C, TR genes.   Results: Out of 200 isolates, 18.5 % were MRSA and 32% were MRCNS (methicillin resistant coagulase negative staphylococci. Of 80 erythromycin resistant isolates, 48 were coagulase negative and 32 were S. aureus. Among the 48 coagulase negative staphylococci (CONS isolates, 11.63% expressed the MLSB-inducible phenotypes. Using PCR, the frequency of different genes in the collection of isolates were as follows: ermA 5.41 % , erm B 5.41 % , and erm C 3.13%. The ermTR gene was negative in all isolates. Among the 32 S. aureus isolates, 9.38% expressed the MLSB-nducible phenotype. Using PCR, these isolates harbored erm A (2.22%, ermB (2.22%, ermC (2.22% and ermTR (2.22% .   Conclusion: This is the first study to show the rate of inducible clindamycin clinical isolates of staphylococci harboring erm genes in Sananadaj. It also demonstrated the frequency of erm genes was higher among CONS isolates than S. aureus. This data suggested the transfer of resistance gene from nonpathogenic to pathogenic strains is likely to happen. Therefore, screening and control of these resistance genes is recommended at clinical laboratories.

  13. Extra-pair copulation and extra-range movements in Flammulated Owls

    Science.gov (United States)

    Richard T. Reynolds; Brian D. Linkhart

    1990-01-01

    We report an extra-pair copulation (EPC) in the Flammulated Owl (Otus flammeolus), the first in strigiforms, and document 21 cases of extra-range movements (ERMs) in this species. Extra-range movements occurred throughout nesting with both sexes participating: males anytime during nesting and females only after their young fledged. Males appear to make ERMs to...

  14. Kahe aastaga omatehtud rahvarõivad selga / Rein Raudvere

    Index Scriptorium Estoniae

    Raudvere, Rein, 1955-

    2011-01-01

    Eesti Rahva Muuseumi juures tegutsevast rahvarõivaste valmistajate koolist. 24. septembril alustas kooli neljas lend. Tutvustavad projektijuht Janne Vaabla, ERM-i teadur-kuraator Reet Piiri jt. Rahvakultuuri Arendus- ja Koolituskeskuse rahvarõivakooli osa õppusi toimub ERM-is

  15. Improving monitoring of erythromycin ribosome methylase genes in swine and cattle manures with gene targeted approaches

    Science.gov (United States)

    Macrolide antibiotics are often used in feed for animal industry to prevent diseases. Resistance to these antibiotics is associated with erythromycin ribosome methylase genes (erm genes), which were first discovered in Staphylococcus aureus. The erm gene confers resistance by methylating rRNA at the...

  16. Improvement of daptomycin yield by overexpression of the ...

    African Journals Online (AJOL)

    The effects of the accessory genes flanking the non-ribosomal peptide synthetase (NRPS) genes on daptomycin production were investigated by overexpression under the control of ermE* promoter via the integrative Escherichia coli–Streptomyces vector pIB139. The yield of daptomycin was promoted significantly when ...

  17. "They Will Post a Law about Playing Soccer" and Other Ethnic/Racial Microaggressions in Organized Activities Experienced by Mexican-Origin Families

    Science.gov (United States)

    Lin, Alex R.; Menjívar, Cecilia; Vest Ettekal, Andrea; Simpkins, Sandra D.; Gaskin, Erin R.; Pesch, Annelise

    2016-01-01

    Organized activities have been found to provide positive experiences for Latino adolescents to develop confidence and learn critical life skills; however, these programs are sometimes a context where youth encounter negative experiences related to ethnic/racial microaggressions (ERMs). This qualitative study explores the types of ERMs that…

  18. Emergent Motivation to Read in Prekindergarten Children

    Science.gov (United States)

    Zheng, Guoguo; Schwanenflugel, Paula J.; Rogers, Samantha M.

    2016-01-01

    This study aimed to develop and validate a measure of emergent reading motivation designed for prekindergarten children, called the Emergent Reading Motivation Scale (ERMS). The development of the ERMS was to overcome the limitation that current existing reading motivation measures are not developmentally appropriate for young children. Fifty-six…

  19. Calibration of the Flow in the Test Section of the Research Wind Tunnel at DST Group

    Science.gov (United States)

    2015-10-01

    1073. Defence Science and Technology Organisation , Melbourne, Australia. Erm, L. P. 2003 Calibration of the flow in the extended test section...of the low-speed wind tunnel at DSTO. DSTO-TR-1384. Defence Science and Technology Organisation , Melbourne, Australia. Erm, L. P. 2015

  20. Linlased saavad valida Tallinna uut logo / Askur Alas

    Index Scriptorium Estoniae

    Alas, Askur, 1973-

    2005-01-01

    Tallinna logo konkursist. Rahvusvahelise žürii poolt valitud 10 finalisti pluss Aavo Ermeli poolt 2003. a. kavandatud logo on hindamiseks veebilehel logo.tallinn.ee. Ermeli logo "mineoletulevik" on praegu kasutusel. Finalistidele annavad hinnangu linnavalitsuse liikmed, rahvusvaheline žürii ja tallinlased SMS-hääletuse teel. Žürii koosseis. Lisatud 11 kavandit

  1. Planning and implementing electronic records management a practical guide

    CERN Document Server

    Smith, Kelvin

    2007-01-01

    Many organizations are moving away from managing records and information in paper form to setting up electronic records management (ERM) systems. Whatever the whyfor in your organization, this book provides straightforward, practical guidance on how to prepare for and enable ERM.

  2. Think Locally: A Prudent Approach to Electronic Resource Management Systems

    Science.gov (United States)

    Gustafson-Sundell, Nat

    2011-01-01

    A few articles have drawn some amount of attention specifically to the local causes of the success or failure of electronic resource management system (ERMS) implementations. In fact, it seems clear that local conditions will largely determine whether any given ERMS implementation will succeed or fail. This statement might seem obvious, but the…

  3. Download this PDF file

    African Journals Online (AJOL)

    NESG PUBLICATIONS

    ised at the regional level to facilitate swift information sharing; provide guidance on issues of common interest; and to put in place enhanced guidelines to strengthen bank's risk management practices, in particular, Enterprise Risk. Management (ERM). ERM gives a firm-wide risk management process to financial institutions.

  4. The adoption and design of Enterprise Risk Management practices : An empirical study

    NARCIS (Netherlands)

    Paape, L.; Speklé, R.F.

    2012-01-01

    We examine (1) the extent of enterprise risk management (ERM) implementation and the factors that are associated with cross-sectional differences in the level of ERM adoption, and (2) specific risk management design choices and their effect on perceived risk management effectiveness. Broadly

  5. Leveraging Enterprise Risk Management: Opportunity for Greater Relevance

    Science.gov (United States)

    Abraham, Janice M.; Baird, Robert; Neugebauer, Frank

    2013-01-01

    Enterprise Risk Management (ERM) gained a foothold in higher education during the first decade of the 21st century. College and university governing board members came to their board service with the perspective and experience of having served on corporate boards and in corporate leadership positions where ERM was in active use. Trustees asked…

  6. Spontaneous release of epiretinal membrane in a young weight-lifting athlete by presumed central rupture and centrifugal pull

    Directory of Open Access Journals (Sweden)

    Mansour AM

    2014-11-01

    Full Text Available Ahmad M Mansour,1,2 Hana A Mansour,3 J Fernando Arevalo4,5 1Department of Ophthalmology, Rafic Hariri University Hospital, Beirut, Lebanon; 2Department of Ophthalmology, American University of Beirut, Beirut, Lebanon; 3Department of Biology, American University of Beirut, Beirut, Lebanon; 4Retina Department, The King Khaled Eye Specialist Hospital, Riyadh, Kingdom of Saudi Arabia; 5Retina Department, Wilmer Eye Institute, The Johns Hopkins University, Baltimore, MD, USA Abstract: This patient presented for surgery at the age of 32 years, 14 months after his initial complaint of metamorphopsia and visual loss in the right eye. Past tests demonstrated a whitish epiretinal membrane (ERM with translucent stress lines over a thickened macula. Visual acuity was found on last presentation to be normal with minimal alteration on Amsler grid testing. A torn ERM was found in the center with left-over ERM temporally and rolled-over ERM nasally at the site of the epicenter with no posterior vitreous detachment. Visual recovery occurred gradually over several days 2 months prior to presentation apparently following heavy weight-lifting with a sensation of severe eye pressure. Sequential funduscopy and optical coherence tomography scans demonstrated the peeling of an ERM accompanied by normalization of foveal thickness. Valsalva maneuver had put excessive tension on ERM which tore in its center at the weakest line with gradual contraction of the ERM away from the fovea towards the peripapillary area. This is a new mechanism of self-separation of ERM induced by Valsalva. ERM in young subjects is subject to rupture and subsequent separation by tangential traction. There are three mechanisms for spontaneous separation of ERM: 1 posterior vitreous detachment with pulling of ERM by detaching vitreous (most common in adults; 2 the contracting forces of the immature ERM become stronger than its adhesions to the retina resulting in slow tangential traction on the

  7. Enterprise risk management in international construction operations

    CERN Document Server

    Zhao, Xianbo; Low, Sui Pheng

    2015-01-01

    This book provides readers an understanding of the implementation of Enterprise Risk Management (ERM) for international construction operations. In an extended case study, it primarily focuses on Chinese construction firms (CCFs) based in Singapore. In this regard, the book explains the differences and similarities between Risk Management (RM), Project Risk Management (PRM) and ERM in the construction industry, and examines their linkages for international construction operations in a broader context. The explanation elaborates on how companies may adopt and implement RM, PRM and ERM as appropriate in their various operations, both in their home market as well as in overseas host markets. The book also reviews the whole spectrum of work relating to organizational behavior (OB) as one of the key underpinnings for companies to evaluate and implement ERM. It will benefit practitioners from the industry as well as academics interested in the implementation of ERM practices in international construction operat...

  8. Disruption of mycorrhizal extraradical mycelium and changes in leaf water status and soil aggregate stability in rootbox-grown trifoliate orange.

    Science.gov (United States)

    Zou, Ying-Ning; Srivastava, A K; Ni, Qiu-Dan; Wu, Qiang-Sheng

    2015-01-01

    Arbuscular mycorrhizas possess well developed extraradical mycelium (ERM) network that enlarge the surrounding soil for better acquisition of water and nutrients, besides soil aggregation. Distinction in ERM functioning was studied under a rootbox system, which consisted of root+hyphae and root-free hyphae compartments separated by 37-μm nylon mesh with an air gap. Trifoliate orange (Poncirus trifoliata) seedlings were inoculated with Funneliformis mosseae in root+hyphae compartment, and the ERM network was established between the two compartments. The ERM network of air gap was disrupted before 8 h of the harvest (one time disruption) or multiple disruptions during seedlings acclimation. Our results showed that mycorrhizal inoculation induced a significant increase in growth (plant height, stem diameter, and leaf, stem, and root biomass) and physiological characters (leaf relative water content, leaf water potential, and transpiration rate), irrespective of ERM status. Easily-extractable glomalin-related soil protein (EE-GRSP) and total GRSP (T-GRSP) concentration and mean weight diameter (MWD, an indicator of soil aggregate stability) were significantly higher in mycorrhizosphere of root+hyphae and root-free hyphae compartments than non-mycorrhizosphere. One time disruption of ERM network did not influence plant growth and soil properties but only notably decreased leaf water. Periodical disruption of ERM network at weekly interval markedly inhibited the mycorrhizal roles on plant growth, leaf water, GRSP production, and MWD in root+hyphae and hyphae chambers. EE-GRSP was the most responsive GRSP fraction to changes in leaf water and MWD under root+hyphae and hyphae conditions. It suggests that effect of peridical disruption of ERM network was more impactful than one-time disruption of ERM network with regard to leaf water, plant growth, and aggregate stability responses, thereby, implying ERM network aided in developing the host plant metabolically more active.

  9. Disruption of mycorrhizal extraradical mycelium and changes in leaf water status and soil aggregate stability in rootbox-grown trifoliate orange

    Directory of Open Access Journals (Sweden)

    Ying-Ning eZou

    2015-03-01

    Full Text Available Arbuscular mycorrhizas possess well developed extraradical mycelium (ERM network that enlarge the surrounding soil for better acquisition of water and nutrients, besides soil aggregation. Distinction in ERM functioning was studied under a rootbox system, which consisted of root+hyphae and root-free hyphae compartments separated by 37-μm nylon mesh with an air gap. Trifoliate orange (Poncirus trifoliata seedlings were inoculated with Funneliformis mosseae in root+hyphae compartment, and the ERM network was established between the two compartments. The ERM network of air gap was disrupted before 8 h of the harvest (one time disruption or multiple disruptions during seedlings acclimation. Our results showed that mycorrhizal inoculation induced a significant increase in growth (plant height, stem diameter, and leaf, stem, and root biomass and physiological characters (leaf relative water content, leaf water potential, and transpiration rate, irrespective of ERM status. Easily-extractable glomalin-related soil protein (EE-GRSP and total GRSP (T-GRSP concentration and mean weight diameter (MWD, an indicator of soil aggregate stability were significantly higher in mycorrhizosphere of root+hyphae and root-free hyphae compartments than non-mycorrhizosphere. One time disruption of ERM network did not influence plant growth and soil properties but only notably decreased leaf water. Periodical disruption of ERM network at weekly interval markedly inhibited the mycorrhizal roles on plant growth, leaf water, GRSP production, and MWD in root+hyphae and hyphae chambers. EE-GRSP was the most responsive GRSP fraction to changes in leaf water and MWD under root+hyphae and hyphae conditions. It suggests that effect of peridical disruption of ERM network was more impactful than one-time disruption of ERM network with regard to leaf water, plant growth, and aggregate stability responses, thereby, implying ERM network aided in developing the host plant metabolically

  10. Prevalence and risk factors of epiretinal membranes: a systematic review and meta-analysis of population-based studies.

    Science.gov (United States)

    Xiao, Wei; Chen, Xiaoyun; Yan, William; Zhu, Zhuoting; He, Mingguang

    2017-09-25

    This study was to aggregate the prevalence and risks of epiretinal membranes (ERMs) and determine the possible causes of the varied estimates. Systematic review and meta-analysis. The search strategy was designed prospectively. We searched PubMed, Embase and Web of Science databases from inception to July 2016. Reference lists of the included literatures were reviewed as well. Surveys published in English language from any population were included if they had a population-based design and reported the prevalence of ERM from retinal photography with or without optical coherence tomography. Eligibility and quality evaluation was conducted independently by two investigators. The literature search generated 2144 records, and 13 population-based studies comprising 49 697 subjects were finally included. The prevalence of ERM and the ORs of potential risk factors (age, sex, myopia, hypertension and so on) were extracted. The pooled age-standardised prevalence estimates of earlier ERM (cellophane macular reflex (CMR)), advanced ERM (preretinal macular fibrosis (PMF)) and any ERM were 6.5% (95% CI 4.2% to 8.9%), 2.6% (95% CI 1.8% to 3.4%) and 9.1% (95% CI 6.0% to 12.2%), respectively. In the subgroup analysis, race and photography modality contributed to the variation in the prevalence estimates of PMF, while the WHO regions and image reading methods were associated with the varied prevalence of CMR and any ERM. Meta-analysis showed that only greater age and female significantly conferred a higher risk of ERMs. Our findings suggest that ERMs are relatively common among aged population. Race, image taking and reading methodology may play important roles in influencing the large variability of ERM prevalence estimates. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  11. Aquaporin-1 Expression in Proliferative Vitreoretinopathy and in Epiretinal Membranes

    Directory of Open Access Journals (Sweden)

    Elie Motulsky

    2014-01-01

    Full Text Available Purpose. Aquaporin-1 (AQP1 is involved in cell migration and proliferation; therefore, the purpose of the study was to investigate its expression in proliferative vitreoretinopathy (PVR and epiretinal membranes (ERM. Methods. 19 membranes from PVR and ERM were collected following eye surgery. AQP1 mRNA and protein expressions were determined by RT-qPCR and immunofluorescence in the membranes from PVR and ERM. Results. AQP1 mRNA and protein were expressed in both PVR and ERM as shown by RT-qPCR and immunofluorescence. AQP1 protein expression was heterogeneous among and between PVR and ERM and colocalized with alpha-smooth muscle actin (αSMA and with glial fibrillary acidic protein (GFAP. There were a higher percentage of cells coexpressing AQP1 and αSMA than AQP1 and GFAP. GFAP and αSMA did not colocalize. Conclusion. Our data show for the first time AQP1 expression in both PVR and ERM. AQP1 is expressed mostly by the αSMA-positive cells, presumably myofibroblasts, but also by GFAP-positive cells, assumed to be glial cells. These original findings warrant further functional investigations aiming at studying the potential role of AQP1 in cell migration and proliferation occurring during the development of PVR and ERM.

  12. [Effects of Thermophilic Composting on Antibiotic Resistance Genes (ARGs) of Swine Manure Source].

    Science.gov (United States)

    Zheng, Ning-guo; Huang, Nan; Wang, Wei-wei; Yu, Man; Chen, Xiao-yang; Yao, Yan-lai; Wang, Wei-ping; Hong, Chun-lai

    2016-05-15

    To investigate the effects of thermophilic composting process on antibiotic resistance genes (ARGs) of swine manure source at a field scale, the abundance of four erythromycin resistance genes (ermA, ermB, ermC and ermF), three β-lactam resistance genes (blaTEM, blaCTX and blaSHV) and two quinolone resistance genes (qnrA and qnrS) were quantified by quantitative PCR ( qPCR) during the composting process. The results suggested that the erm genes' copy numbers were significantly higher than those of the bla and qnr genes in the early stage of composting (P composting process, bla and qnr genes were at low levels, while erm genes were still at high levels. Even through ermF was proliferated comparing with the initial copies. These results indicated that thermophilic composting process could not effectively remove all ARGs. For some ARGs, compost may be a good bioreactor resulting in their proliferation. Application of composting products on farmland may cause transference of ARGs.

  13. Changes in turnover rather than production regulate biomass of ectomycorrhizal fungal mycelium across a Pinus sylvestris chronosequence.

    Science.gov (United States)

    Hagenbo, Andreas; Clemmensen, Karina E; Finlay, Roger D; Kyaschenko, Julia; Lindahl, Björn D; Fransson, Petra; Ekblad, Alf

    2017-04-01

    In boreal forest soils, ectomycorrhizal fungi are fundamentally important for carbon (C) dynamics and nutrient cycling. Although their extraradical mycelium (ERM) is pivotal for processes such as soil organic matter build-up and nitrogen cycling, very little is known about its dynamics and regulation. In this study, we quantified ERM production and turnover, and examined how these two processes together regulated standing ERM biomass in seven sites forming a chronosequence of 12- to 100-yr-old managed Pinus sylvestris forests. This was done by determining ERM biomass, using ergosterol as a proxy, in sequentially harvested in-growth mesh bags and by applying mathematical models. Although ERM production declined with increasing forest age from 1.2 to 0.5 kg ha-1  d-1 , the standing biomass increased from 50 to 112 kg ha-1 . This was explained by a drastic decline in mycelial turnover from seven times to one time per year with increasing forest age, corresponding to mean residence times from 25 d up to 1 yr. Our results demonstrate that ERM turnover is the main factor regulating biomass across differently aged forest stands. Explicit inclusion of ERM parameters in forest ecosystem C models may significantly improve their capacity to predict responses of mycorrhiza-mediated processes to management and environmental changes. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

  14. A New Approach for the Determination of Dose Rate and Radioactivity for Detected Gamma Nuclides Using an Environmental Radiation Monitor Based on an NaI(Tl) Detector.

    Science.gov (United States)

    Ji, Young-Yong; Kim, Chang-Jong; Lim, Kyo-Sun; Lee, Wanno; Chang, Hyon-Sock; Chung, Kun Ho

    2017-10-01

    To expand the application of dose rate spectroscopy to the environment, the method using an environmental radiation monitor (ERM) based on a 3' × 3' NaI(Tl) detector was used to perform real-time monitoring of the dose rate and radioactivity for detected gamma nuclides in the ground around an ERM. Full-energy absorption peaks in the energy spectrum for dose rate were first identified to calculate the individual dose rates of Bi, Ac, Tl, and K distributed in the ground through interference correction because of the finite energy resolution of the NaI(Tl) detector used in an ERM. The radioactivity of the four natural radionuclides was then calculated from the in situ calibration factor-that is, the dose rate per unit curie-of the used ERM for the geometry of the ground in infinite half-space, which was theoretically estimated by Monte Carlo simulation. By an intercomparison using a portable HPGe and samples taken from the ground around an ERM, this method to calculate the dose rate and radioactivity of four nuclides using an ERM was experimentally verified and finally applied to remotely monitor them in real-time in the area in which the ERM had been installed.

  15. Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study

    Directory of Open Access Journals (Sweden)

    Dorota Romaniuk

    2013-01-01

    Full Text Available The molecular mechanism formation of secondary epiretinal membranes (ERMs after proliferative diabetic retinopathy (PDR or primary idiopathic ERMs is still poorly understood. Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patients with PDR. The examined group comprised 6 patients with secondary ERMs after PDR and the control group consisted of 11 patients with idiopathic ERMs. Quantification of IGF1, IGF1R, and IGFBP3 mRNAs was performed by real-time QRT-PCR technique. In ERMs, IGF1 and IGF1R mRNA levels were significantly higher in patients with diabetes compared to control subjects. In PBMCs, there were no statistically significant differences of IGF1, IGF1R, and IGFBP3 expression between diabetic and nondiabetic patients. In conclusion, our study indicated IGF1 and IGF1R differential expression in ERMs, but not in PBMCs, of diabetic and nondiabetic patients, suggesting that these factors can be involved in the pathogenesis or progression of proliferative vitreoretinal disorders. This trial is registered with NCT00841334.

  16. Spectral versus time-domain OCT in detecting preoperative epiretinal membranes that accompany macular holes.

    Science.gov (United States)

    Rao, Prethy; Yonekawa, Yoshihiro; Thomas, Benjamin J; Drenser, Kimberly A

    2017-03-10

    To compare the sensitivities of spectral-domain optical coherence tomography (SD-OCT) versus time-domain OCT (TD-OCT) in identifying epiretinal membranes (ERM) preoperatively in patients who underwent surgery for full-thickness macular holes (FTMH). This is an interventional retrospective case series of 59 eyes diagnosed with FTMHs who underwent 25-G pars plana vitrectomy with internal limiting membrane peeling between 2009 and 2015. Preoperative OCTs were obtained by SD-OCT (Spectralis, Heidelberg, Germany) or TD-OCT (Stratus, Carl Zeiss Meditec, Dublin, CA, USA). Volume scans were reviewed for ERM accompanying the FTMH. This was compared to indocyanine green-negative staining and intraoperative findings of ERM as the gold standard. Baseline characteristics between the SD-OCT and TD-OCT groups were comparable. Mean duration of postoperative follow-up was 41.4 weeks (±49.0). Of 59 eyes, 33 (55.9%) exhibited an ERM intraoperatively. Four ERMs (SD-OCT group) compared to 12 (TD-OCT group) were not visualized on preoperative OCT (p = 0.003). Sensitivity and specificity of SD-OCT in ERM detection was 79% and 100% compared to 14% and 91% for TD-OCT. Visual acuity improved in both arms (0.5 and 0.3 logMAR units in SD-OCT and TD-OCT, respectively (p = 0.002, 0.0002). We found that SD-OCT was superior to TD-OCT in identifying the presence of ERM preoperatively in patients who underwent macular hole surgery. Since ERMs may decrease the chance of successful pharmacologic vitreolysis, we recommend using SD-OCT over TD-OCT in the evaluation of patients with FTMH to more accurately identify ERMs and allow more comprehensive treatment decisions (pharmacologic versus surgical).

  17. Rapid maxillary expansion with Haas and the speech in people with cleft lip and palate

    OpenAIRE

    Christiane Moraes Lazzari; Márcia Keske-Soares; Carolina Lisbôa Mezzomo

    2009-01-01

    OBJETIVO: verificar os efeitos da Expansão Rápida da Maxila (ERM), com aparelho Haas, na fala do paciente com fissura labiopalatina (FLP) Transforame. MÉTODOS: 12 indivíduos entre sete e 14 anos, sendo seis meninos e seis meninas, divididos em dois grupos. O Grupo 1 (G1), submetido à ERM com protocolo de ativação convencional e, o Grupo 2 (G2), submetido à ERM com protocolo de ativação preconizado por Liou e Tsai (2005) com expansões e constrições alternadas. Realizou-se avaliação da fala, an...

  18. Electronic resource management systems a workflow approach

    CERN Document Server

    Anderson, Elsa K

    2014-01-01

    To get to the bottom of a successful approach to Electronic Resource Management (ERM), Anderson interviewed staff at 11 institutions about their ERM implementations. Among her conclusions, presented in this issue of Library Technology Reports, is that grasping the intricacies of your workflow-analyzing each step to reveal the gaps and problems-at the beginning is crucial to selecting and implementing an ERM. Whether the system will be used to fill a gap, aggregate critical data, or replace a tedious manual process, the best solution for your library depends on factors such as your current soft

  19. Epiretinal membrane removal in patients with Stargardt disease

    Directory of Open Access Journals (Sweden)

    Muna Bhende

    2015-01-01

    Full Text Available Epiretinal membranes (ERMs in Stargardt disease have been known to undergo spontaneous separation in children. Results of surgical intervention in adult patients with Stargardt disease have rarely been reported. A retrospective review of results of surgical intervention for ERM causing visual impairment in two adult patients of Stargardt disease was carried out. Both patients developed ERM in one eye during their follow-up period with the resultant drop in their preexisting visual acuity. Postsurgery, restoration of foveal contour with some improvement in visual acuity was observed in both patients. No adverse effect of surgery was noted.

  20. Identifying risks in the realm of enterprise risk management.

    Science.gov (United States)

    Carroll, Roberta

    2016-01-01

    An enterprise risk management (ERM) discipline is comprehensive and organization-wide. The effectiveness of ERM is governed in part by the strength and breadth of its practices and processes. An essential element in decision making is a thorough process by which organizational risks and value opportunities can be identified. This article will offer identification techniques that go beyond those used in traditional risk management programs and demonstrate how these techniques can be used to identify risks and opportunity in the ERM environment. © 2016 American Society for Healthcare Risk Management of the American Hospital Association.

  1. Global Gravity Grids, Geoid Height and Gravity Anomaly Profiles

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The entire collection of GEOSAT ERM (Nov.'86 - Dec. '89) data over land and ice regions is held at the National Geophysical Data Center (NGDC). These data will yield...

  2. Experiment list: SRX188972 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid cell line, Clinically normal; 4 paternal cousins have Cornelia de Lange syndr...sm=human || cell description=lymphoblastoid cell line, Clinically normal; 4 paternal cousins have Cornelia d

  3. Experiment list: SRX190020 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Ba...B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah, treatment:

  4. Experiment list: SRX150527 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, Japanese in Tokyo, Japan, treatment: Epstein-B...1 || cell organism=human || cell description=lymphoblastoid, International HapMap Project, Japanese in Tokyo

  5. Experiment list: SRX190042 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European

  6. Experiment list: SRX150492 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...an || cell description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Cau

  7. Experiment list: SRX150470 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...ll organism=human || cell description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah

  8. Experiment list: SRX150718 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Barr Virus trans...uman || cell description=lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Barr Vi

  9. Uudised : "Vox septem saeculi". XI "Jazzkaar", esimene sel sajandil / Mattisen Tiina

    Index Scriptorium Estoniae

    Mattisen Tiina

    2000-01-01

    T. Kaljuste tutvustas 1. märtsil toimunud pressikonverentsil EFK uut kontserdisarja "Vox septem saeculi". 1. märtsil toimunud pressikonverentsil tutvustas A. Erm 23.-30. apr. toimuva XI "Jazzkaare" programmi

  10. The impacts of the power plant generation using biomass in the price of energy in Brazil and its incorporation to the mechanism for reallocation of energy; Os impactos da geracao de usinas a biomassa no preco da energia no Brasil e sua incorporacao ao mecanismo de realocacao de energia

    Energy Technology Data Exchange (ETDEWEB)

    Tardin, Thiago V.; Souza, Luiz Eduardo de; Ribeiro, Paulo [Universidade Federal de Juiz de Fora (UFJF), MG (Brazil)], Emails: thiago@engenho.com, luizeduardo_jf@yahoo.com.br, pfribeiro@ieee.org; Leite, Paula Beatriz Cerqueira [Engenho Pesquisa, Desenvolvimento e Consultoria Ltda, Rio de Janeiro RJ (Brazil)], E-mail: Paula@engenho.com

    2009-07-01

    The energy reallocation mechanism (ERM) is a compulsory scheme used to reduce the hydrology risk. The aim of this paper is to present a proposed merger of biomass power plants in ERM. This merger will bring great benefits to the power system as a whole in that the generation of biomass is complementary to the system, reducing thus, the hydrological risk and improve safety of supply, but without raising the price of energy, since that the variable cost of production of biomass is very down. Furthermore, biomass has also benefits to be incorporated into the ERM, as it may commercialize energy to a wider range of buyers, not only during the harvest period, but also in the off season, since the ERM 'donate' energy during the period between harvests, reducing the risk of exposure to the spot market.

  11. Experiment list: SRX188971 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid cell line, clinically normal; monozygotic twin sister with Cornelia De Lange ... cell description=lymphoblastoid cell line, clinically normal; monozygotic twin sister with Cornelia De Lang

  12. Geosat Geodetic Mission Waveform Data Records (WDR) for April, 1986 (NODC Accession 0002561)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains one month of waveform data records (WDRs) from the GEOSAT Geodetic Mission (GM) and(or) Exact Repeat Mission (ERM) for the time period of...

  13. Geosat Geodetic Mission Waveform Data Records (WDR) for June, 1986 (NODC Accession 0002563)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains one month of waveform data records (WDRs) from the GEOSAT Geodetic Mission (GM) and(or) Exact Repeat Mission (ERM) for the time period of...

  14. Karulaugu lasteaed-algkool = Karulaugu preschool and elementary school / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    2009-01-01

    2009. a. Haabneemes Randvere tee 18 valminud Karulaugu lasteaed-algkoolist, mille arhitektid on Emil Urbel ja Indrek Erm, projekteerija AB Emil Urbel, sisekujundajad Taso Mähar ja Ines Haak. Projekt 2007

  15. Vaccination against enteric red mouth disease caused by the bacterium Yersinia ruckeri

    DEFF Research Database (Denmark)

    Raida, Martin Kristian

    Recently the Danish research council has granted the project:”Understanding key mechanisms in mucosal immunity: Development of a new immersion vaccine”. The main objective of the project is to develop a new vaccine which is long lasting and protective against both Y.ruckeri biotype 1 and a European...... isolate of biotype 2. Recently a new biotype (biotype 2) of Y. ruckeri has been isolated from farmed trout diagnosed with ERM across Europe although the fish had been vaccinated against ERM with the commercially available ERM-vaccine. Further, we will develop a new vaccination strategy based...... on the hypothesis that booster vaccinations will improve and prolong the protection of the rainbow trout against ERM. The vaccine induced protection will be tested in laboratory bath challenge infections before and after booster vaccination. It is the goal that the effect of the experimental vaccine and the most...

  16. Global 3D imaging of Yersinia ruckeri bacterin uptake in rainbow trout fry

    DEFF Research Database (Denmark)

    Otani, Maki; Villumsen, Kasper Rømer; Koppang, Erling Olaf

    2015-01-01

    vaccination. Later, bacterin uptake was detected on other mucosal surfaces such as skin and olfactory bulb from 5 to 30 minutes post vaccination. The GI tract was found to be filled with a complex of bacterin and mucus at 3 hours post vaccination and the bacterin remained in the GI tract for at least 24 hours......Yersinia ruckeri is the causative agent of enteric redmouth disease (ERM) in rainbow trout, and the first commercially available fish vaccine was an immersion vaccine against ERM consisting of Y. ruckeri bacterin. The ERM immersion vaccine has been successfully used in aquaculture farming...... of salmonids for more than 35 years. The gills and the gastrointestinal (GI) tract are believed to be the portals of antigen uptake during waterborne vaccination against ERM; however, the actual sites of bacterin uptake are only partly understood. In order to obtain insight into bacterin uptake during...

  17. See kummaline kohtumispaik Las Vegas : intervjuu Johnny Deppiga / intervjueerinud Jaques-André Bondy ; tõlkinud Kaia Sisask

    Index Scriptorium Estoniae

    Depp, Johnny, 1963-

    1998-01-01

    Näitleja oma rollist Terry Gilliami mängufilmis "Ratastel Las Vegases" (Suurbritannia-USA, 1998), tõlge ajakirjast "Premiere", 1998, juuni; lisa : A. Ermeli koostatud ülevaade J. Deppi olulisematest filmirollidest

  18. Kollaboratives Trendmanagement

    National Research Council Canada - National Science Library

    Durst, Michael; Stang, Stefanie; Stößer, Lena; Edelmann, Fritz

    2010-01-01

    .... Der vorgestellte Ansatz ermöglicht weiterhin eine Visualisierung der Trends zur Entscheidungsunterstützung und Fokussierung. Ein Praxisbeispiel der Scout24 Holding GmbH demonstriert den Einsatz der Methode in der Praxis.

  19. Epithelial rests of Malassez: from latent cells to active participation in orthodontic movement

    Directory of Open Access Journals (Sweden)

    Bianca Silva e Silva

    Full Text Available ABSTRACT Introduction: The epithelial rests of Malassez (ERM represent a group of cells in the periodontal ligament classically consisting of latent or quiescent structures associated with pathological processes. However, recent evidence shows that these structures cannot be considered only as cellular debris. The ERM is a major tissue structure, with functions in maintaining the homeostasis of periodontal tissue, including the maintenance of orthodontic movement. Objective: The present literature review aims at presenting the potential functions of ERM, with emphasis on orthodontic movement and the functional structure of the periodontium. Conclusion: ERM cells have a functional activity in modulation of orthodontic movement, trough their potential for differentiation, maintenance functions and the capacity of repairing periodontium.

  20. Experiment list: SRX150686 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid cell line, Instability of heterochromatin of chromosomes 1, 9, and 16 with variable combined...ll line, Instability of heterochromatin of chromosomes 1, 9, and 16 with variable combined immunodeficiency;

  1. Pärnu Kontserdimaja arhitektuurikonkurss = Architectural Competition of Pärnu Concert Hall / Martti Preem

    Index Scriptorium Estoniae

    Preem, Martti, 1949-

    2001-01-01

    Asukohavalikust, premeeritud ja äramärgitud töödest. Konkursi tulemused: I preemia - Hanno Grossschmidt, Katrin Koov, Kaire Nõmm, Üla Koppel; II - Indrek Erm, Emil Urbel; III - Kalle Komissarov, Andres Ojari. 21 ill.

  2. Geosat Exact Repeat Mission Waveform Data Records (WDR) (NODC Accession 0061150)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains waveform data records (WDRs) from the US Navy Geodetic Satellite (GEOSAT) Exact Repeat Mission (ERM) for the time period of November 08, 1986...

  3. Geosat Geodetic Mission Waveform Data Records (WDR) for May, 1985 (NODC Accession 0002365)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains one month of waveform data records (WDRs) from the GEOSAT Geodetic Mission (GM) and(or) Exact Repeat Mission (ERM) for the time period of May...

  4. Drug: D05714 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 902+2903+2904+2905+2906) Neuroactive ligand-receptor interaction hsa04720(2902+2903+2904+2905+2906) Long-t...erm potentiation hsa04724(2902+2903+2904+2905+2906) Glutamatergic synapse Target-ba

  5. Prime Hook National Wildlife Refuge contaminants study: Examining the impacts of Clifton Cannery, a potential point source

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Sediment Data Analytical results for sediment samples collected from the unnamed creek and "clean" Delaware background areas are compared to ER-M levels in Table 1....

  6. Calcitriol Inhibits Hedgehog Signaling and Induces Vitamin D Receptor Signaling and Differentiation in the Patched Mouse Model of Embryonal Rhabdomyosarcoma

    Directory of Open Access Journals (Sweden)

    Anja Uhmann

    2012-01-01

    Full Text Available Rhabdomyosarcoma (RMS is the most common soft tissue sarcoma in children. Aberrant Hedgehog (Hh signaling is characteristic of the embryonal subtype (ERMS and of fusion-negative alveolar RMS. In the mouse, ERMS-like tumors can be induced by mutations in the Hh receptor Patched1 (Ptch. As in humans these tumors show increased Hh pathway activity. Here we demonstrate that the treatment with the active form of vitamin D3, calcitriol, inhibits Hh signaling and proliferation of murine ERMS in vivo and in vitro. Concomitantly, calcitriol activates vitamin D receptor (Vdr signaling and induces tumor differentiation. In addition, calcitriol inhibits ERMS growth in Ptch-mutant mice, which is, however, a rather late response. Taken together, our results suggest that exogenous supply of calcitriol could be beneficial in the treatment of RMS, especially in those which are associated with aberrant Hh signaling activity.

  7. Experiment list: SRX150534 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Nigera, treatment: Epstein-B...9 || cell organism=human || cell description=lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Niger

  8. Experiment list: SRX150402 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Nigera, treatment: Epstein-B...cell organism=human || cell description=lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Niger

  9. Experiment list: SRX150404 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Nigera, treatment: Epstein-B...cell organism=human || cell description=lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Niger

  10. Experiment list: SRX150533 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Nigera, treatment: Epstein-B...3 || cell organism=human || cell description=lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Niger

  11. Experiment list: SRX150607 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, Japanese in Tokyo, Japan, treatment: Epstein-B...cell organism=human || cell description=lymphoblastoid, International HapMap Project, Japanese in Tokyo, Jap

  12. Experiment list: SRX190017 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme...l description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Ut

  13. Experiment list: SRX150529 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...ell organism=human || cell description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Uta

  14. Sensor Data Records (SDR) from the GEOSAT Exact Repeat Mission from 8 November 1986 to 31 December 1989 (NODC Accession 0054289)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains the sensor data records (SDRs) from the Geodetic Satellite (GEOSAT) Exact Repeat Mission (ERM) for the time period of November 8, 1986 to...

  15. Combinations of macrolide resistance determinants in field isolates of Mannheimia haemolytica and Pasteurella multocida

    DEFF Research Database (Denmark)

    Desmolaize, Benoit; Rose, Simon; Wilhelm, Cornelia

    2011-01-01

    )] that are arranged in tandem and presumably expressed from the same promoter. The third class exhibits the most marked drug phenotype, with high resistance to all of the macrolides tested, and possesses all three resistance determinants. The combinations of erm(42), msr(E), and mph(E) are chromosomally encoded...... of the erm(42) gene. The second class shows no lincosamide resistance and lacks erm(42) and concomitant 23S rRNA methylation. Sequencing of the genome of a representative strain from this class, P. multocida 3361, revealed macrolide efflux and phosphotransferase genes [respectively termed msr(E) and mph(E...... and intermingled with other exogenous genes, many of which appear to have been transferred from other members of the Pasteurellaceae. The presence of some of the exogenous genes explains recent reports of resistance to additional drug classes. We have expressed recombinant versions of the erm(42), msr(E), and mph(E...

  16. Bobby heitis Bert Pringile väljakutse / Aigi Viira

    Index Scriptorium Estoniae

    Viira, Aigi

    2004-01-01

    Laulja Bert Pringi, kelle kehastada on suur roll Smithbridge Productions uues muusikaliprojektis - George Gershwini "Hull sinu järele", lavastaja Andrus Vaarik. Ka näitleja Andero Ermeli rollist loobumise põhjustest

  17. Experiment list: SRX150604 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, Han Chinese in Beijing, China, treatment: Epst...26 || cell organism=human || cell description=lymphoblastoid, International HapMap Project, Han Chinese in B

  18. Experiment list: SRX100391 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,... description=Chromatin IP Sequencing || cell description=B-lymphocyte, lymphoblastoid, International HapMap

  19. Experiment list: SRX259074 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm || DIFFERENTIATION_METHOD=adherence || BATCH=BioSAli 1890 || BIOMATERIAL_TYPE=Cell Line || DIFFERENTIATI...ON_STAGE=intermediate || SEX=Male || BIOMATERIAL_PROVIDER=Harvard || sample_term_id=CL_0000221 http://dbarch

  20. Development of a risk-based environmental management tool for drilling discharges. Summary of a four-year project.

    Science.gov (United States)

    Singsaas, Ivar; Rye, Henrik; Frost, Tone Karin; Smit, Mathijs G D; Garpestad, Eimund; Skare, Ingvild; Bakke, Knut; Veiga, Leticia Falcao; Buffagni, Melania; Follum, Odd-Arne; Johnsen, Ståle; Moltu, Ulf-Einar; Reed, Mark

    2008-04-01

    This paper briefly summarizes the ERMS project and presents the developed model by showing results from environmental fates and risk calculations of a discharge from offshore drilling operations. The developed model calculates environmental risks for the water column and sediments resulting from exposure to toxic stressors (e.g., chemicals) and nontoxic stressors (e.g., suspended particles, sediment burial). The approach is based on existing risk assessment techniques described in the European Union technical guidance document on risk assessment and species sensitivity distributions. The model calculates an environmental impact factor, which characterizes the overall potential impact on the marine environment in terms of potentially impacted water volume and sediment area. The ERMS project started in 2003 and was finalized in 2007. In total, 28 scientific reports and 9 scientific papers have been delivered from the ERMS project (http://www.sintef.no/erms).

  1. Experiment list: SRX190043 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme...ll description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/U

  2. Recommendation Report: EJournals/EBooks A-Z Management System

    KAUST Repository

    Ramli, Rindra M.

    2014-01-01

    This is a recommendation report for KAUST Library on the Ejournals / EBooks AZ Management systems project. It briefly described the issues faced by the ERM Team, project plan overview and the project findings as well as the recommendation(s).

  3. Experiment list: SRX080332 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...f=GM12878 || cell=GM12878 || cell organism=Human || cell description=lymphoblastoid, International HapMap Pr

  4. Experiment list: SRX150528 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, Han Chinese in Beijing, China, treatment: Epst...18526 || cell organism=human || cell description=lymphoblastoid, International HapMap Project, Han Chinese i

  5. Experiment list: SRX150532 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatme... || cell=GM12891 || cell organism=human || cell description=B-lymphocyte, lymphoblastoid, International HapM

  6. Experiment list: SRX067493 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...tion=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Ba

  7. Experiment list: SRX150467 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatme...l=GM12891 || cell organism=human || cell description=B-lymphocyte, lymphoblastoid, International HapMap Proj

  8. Experiment list: SRX150643 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...|| cell organism=human || cell description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH

  9. Experiment list: SRX102991 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...Sequencing || cell description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - Euro

  10. Experiment list: SRX100396 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...pe description=Chromatin IP Sequencing || cell description=B-lymphocyte, lymphoblastoid, International HapMa

  11. Experiment list: SRX190021 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme...l description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Ut

  12. Experiment list: SRX150530 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Barr Virus trans...ism=human || cell description=lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Ba

  13. Experiment list: SRX080364 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Ba...f=GM12801 || cell=GM12801 || cell organism=Human || cell description=B-Lymphocyte, Lymphoblastoid, International

  14. Experiment list: SRX150366 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatme...l=GM12892 || cell organism=human || cell description=B-lymphocyte, lymphoblastoid, International HapMap Proj

  15. Vennalik maja = A Brotherly House / Liina Jänes

    Index Scriptorium Estoniae

    Jänes, Liina, 1977-

    2002-01-01

    2001. a. Tallinnas Pirita teel valminud eramu projekteeris Arhitektibüroo Emil Urbel. Arhitektid Emil Urbel ja Indrek Erm, sisearhitekt Taso Mähar. Konstruktsioonid: E-Inseneribüroo. 5 ill.: korruste plaanid, sisevaade, välisvaated

  16. Geosat Geodetic Mission Waveform Data Records (WDR) for January, 1986 (NODC Accession 0002558)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains one month of waveform data records (WDRs) from the GEOSAT Geodetic Mission (GM) and(or) Exact Repeat Mission (ERM) for the time period of...

  17. Prevalence and risk factors for epiretinal membrane: the Singapore Epidemiology of Eye Disease study.

    Science.gov (United States)

    Cheung, Ning; Tan, Shu-Pei; Lee, Shu Yen; Cheung, Gemmy Chui Ming; Tan, Gavin; Kumar, Neelam; Cheng, Ching-Yu; Wong, Tien Yin

    2017-03-01

    To examine prevalence and risk factors of epiretinal membrane (ERM) in a large, contemporary, multiethnic Asian population. Combined analysis of three population-based studies of eye diseases, with a total of 9799 Chinese, Malays and Indians residing in the general communities of Singapore. A comprehensive ophthalmic examination, interviews and laboratory blood tests were performed to assess potential risk factors. Digital retinal photographs were used to assess ERM according to a standardised protocol. ERM was classified into cellophane macular reflex (CMR) and/or preretinal macular fibrosis (PMF), and also as primary or secondary (in eyes with other retinal pathology or a history of cataract surgery). The age-standardised and ethnicity-standardised prevalence was 12.1% for any ERM, 6.8% for CMR, 6.7% for PMF and 2.8% for bilateral ERM. ERM prevalence was higher in Chinese (13.0%) compared with Malays (7.9%) or Indians (8.7%). In multivariate analysis, significant factors associated with primary ERM were older age (OR 1.08 per year increase; p<0.01), Chinese ethnicity (OR 1.60 vs Indians; p<0.01; OR 1.39 vs Malays; p<0.01), smoking (OR 0.70; p=0.01), longer axial length (OR 1.07 per mm increase; p=0.03) and cataract (OR 0.64; p<0.01). Significant factors independently associated with secondary ERM were older age (OR 1.05; p<0.01), cataract surgery (OR 10.6; p<0.01) and diabetic retinopathy (OR 2.48; p<0.01). ERM is common in Asians, particularly among Chinese. Older age is the most consistent risk factor for any ERM, and previous cataract surgery and diabetic retinopathy are the strongest risk factors for secondary ERM. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  18. Novel Root-Fungus Symbiosis in Ericaceae: Sheathed Ericoid Mycorrhiza Formed by a Hitherto Undescribed Basidiomycete with Affinities to Trechisporales

    Science.gov (United States)

    Vohník, Martin; Sadowsky, Jesse J.; Kohout, Petr; Lhotáková, Zuzana; Nestby, Rolf; Kolařík, Miroslav

    2012-01-01

    Ericaceae (the heath family) are widely distributed calcifuges inhabiting soils with inherently poor nutrient status. Ericaceae overcome nutrient limitation through symbiosis with ericoid mycorrhizal (ErM) fungi that mobilize nutrients complexed in recalcitrant organic matter. At present, recognized ErM fungi include a narrow taxonomic range within the Ascomycota, and the Sebacinales, basal Hymenomycetes with unclamped hyphae and imperforate parenthesomes. Here we describe a novel type of basidiomycetous ErM symbiosis, termed ‘sheathed ericoid mycorrhiza’, discovered in two habitats in mid-Norway as a co-dominant mycorrhizal symbiosis in Vaccinium spp. The basidiomycete forming sheathed ErM possesses clamped hyphae with perforate parenthesomes, produces 1- to 3-layer sheaths around terminal parts of hair roots and colonizes their rhizodermis intracellularly forming hyphal coils typical for ErM symbiosis. Two basidiomycetous isolates were obtained from sheathed ErM and molecular and phylogenetic tools were used to determine their identity; they were also examined for the ability to form sheathed ErM and lignocellulolytic potential. Surprisingly, ITS rDNA of both conspecific isolates failed to amplify with the most commonly used primer pairs, including ITS1 and ITS1F + ITS4. Phylogenetic analysis of nuclear LSU, SSU and 5.8S rDNA indicates that the basidiomycete occupies a long branch residing in the proximity of Trechisporales and Hymenochaetales, but lacks a clear sequence relationship (>90% similarity) to fungi currently placed in these orders. The basidiomycete formed the characteristic sheathed ErM symbiosis and enhanced growth of Vaccinium spp. in vitro, and degraded a recalcitrant aromatic substrate that was left unaltered by common ErM ascomycetes. Our findings provide coherent evidence that this hitherto undescribed basidiomycete forms a morphologically distinct ErM symbiosis that may occur at significant levels under natural conditions, yet remain

  19. Novel root-fungus symbiosis in Ericaceae: sheathed ericoid mycorrhiza formed by a hitherto undescribed basidiomycete with affinities to Trechisporales.

    Directory of Open Access Journals (Sweden)

    Martin Vohník

    Full Text Available Ericaceae (the heath family are widely distributed calcifuges inhabiting soils with inherently poor nutrient status. Ericaceae overcome nutrient limitation through symbiosis with ericoid mycorrhizal (ErM fungi that mobilize nutrients complexed in recalcitrant organic matter. At present, recognized ErM fungi include a narrow taxonomic range within the Ascomycota, and the Sebacinales, basal Hymenomycetes with unclamped hyphae and imperforate parenthesomes. Here we describe a novel type of basidiomycetous ErM symbiosis, termed 'sheathed ericoid mycorrhiza', discovered in two habitats in mid-Norway as a co-dominant mycorrhizal symbiosis in Vaccinium spp. The basidiomycete forming sheathed ErM possesses clamped hyphae with perforate parenthesomes, produces 1- to 3-layer sheaths around terminal parts of hair roots and colonizes their rhizodermis intracellularly forming hyphal coils typical for ErM symbiosis. Two basidiomycetous isolates were obtained from sheathed ErM and molecular and phylogenetic tools were used to determine their identity; they were also examined for the ability to form sheathed ErM and lignocellulolytic potential. Surprisingly, ITS rDNA of both conspecific isolates failed to amplify with the most commonly used primer pairs, including ITS1 and ITS1F + ITS4. Phylogenetic analysis of nuclear LSU, SSU and 5.8S rDNA indicates that the basidiomycete occupies a long branch residing in the proximity of Trechisporales and Hymenochaetales, but lacks a clear sequence relationship (>90% similarity to fungi currently placed in these orders. The basidiomycete formed the characteristic sheathed ErM symbiosis and enhanced growth of Vaccinium spp. in vitro, and degraded a recalcitrant aromatic substrate that was left unaltered by common ErM ascomycetes. Our findings provide coherent evidence that this hitherto undescribed basidiomycete forms a morphologically distinct ErM symbiosis that may occur at significant levels under natural conditions, yet

  20. Prevalence of macrolide resistance genes in clinical isolates of the Streptococcus anginosus ("S. milleri") group.

    Science.gov (United States)

    Jacobs, J A; van Baar, G J; London, N H; Tjhie, J H; Schouls, L M; Stobberingh, E E

    2001-08-01

    Twenty-two unrelated erythromycin-resistant anginosus group strains (3.2% resistance rate) were assessed for mechanisms of resistance. Streptococcus anginosus accounted for 16 of the 22 isolates. Fifteen isolates harbored the erm(B) gene. The erm(TR) and the mef(E) genes were carried by two isolates each. In three isolates, none of these resistance genes was detected by PCR.

  1. Genotyping of erythromycin resistant group C & G streptococci isolated in Chennai, south India

    Science.gov (United States)

    Prabu, D.; Menon, Thangam

    2013-01-01

    Background & objectives: Increasing resistance to erythromycin has been observed worldwide in group C and group G streptococci (GCS/GGS). The information available from India is scanty. The aim of the study was to identify erythromycin resistant GCS/GGS isolates in Chennai, south India, and to compare erythromycin resistant genotypes with emm types. Methods: One hundred and thirty one GCS/GGS isolates were tested for erythromycin resistance by disc diffusion and agar dilution methods. Erythromycin resistance genotypes [erm(A), erm(B) and mef(A)] were determined by a multiplex PCR. emm types of erythromycin resistant GCS/GGS isolates was also assessed using emm gene sequencing method. Results: Sixteen of the 131 isolates (12.21%) were resistant to erythromycin. Majority of the isolates were GGS (15/16). Eight of the 16 (50%) were S. dysgalactiae subsps. equisimilis. Twelve isolates (75%) were MLSB phenotype and four (25%) were M phenotype. Of the 12 isolates which exhibited MLSB resistance, seven showed cMLSB phenotype and were positive for erm(B) gene. The remaining five were iMLSB phenotype of which three were positive for erm(A) gene and two for erm(B) gene. erm(A) was common among carriers whereas erm(B) was common among clinical isolates. Interpretation & conclusions: MLSB was the predominant phenotype and erm(B) was the common genotype in the present study. The emm type stC1400.0 was frequently associated with erythromycin resistant GCS/GGS in our study. PMID:23481067

  2. Enterprise Risk Management "More than a Concept"

    OpenAIRE

    el baradei, mostafa

    2006-01-01

    The aim of this dissertation is to explore a new area of risk management where risk is the center of the organization decisions. ERM is a flexible concept that needs knowledge and thinking more than money and resources. The dissertation presents a true application of the ERM on a small company. It proves that "Thinking risk" can be applied in a small organization and can play a major role in its success.

  3. Antimicrobial Susceptibility of Bacillus Strains Isolated from Primary Starters for African Traditional Bread Production and Characterization of the Bacitracin Operon and Bacitracin Biosynthesis

    Science.gov (United States)

    Sørensen, Kim I.; Thorsen, Line; Stuer-Lauridsen, Birgitte; Abdelgadir, Warda S.; Nielsen, Dennis S.; Derkx, Patrick M. F.; Jespersen, Lene

    2012-01-01

    Bacillus spp. are widely used as feed additives and probiotics. However, there is limited information on their resistance to various antibiotics, and there is a growing concern over the transfer of antibiotic resistance genes. The MIC for 8 antibiotics was determined for 85 Bacillus species strains, Bacillus subtilis subsp. subtilis (n = 29), Bacillus licheniformis (n = 38), and Bacillus sonorensis (n = 18), all of which were isolated from starters for Sudanese bread production. All the strains were sensitive to tetracycline (8.0 mg/liter), vancomycin (4.0 mg/liter), and gentamicin (4.0 mg/liter) but resistant to streptomycin. Sensitivity to clindamycin, chloramphenicol, and kanamycin was species specific. The erythromycin resistance genes ermD and ermK were detected by PCR in all of the erythromycin-resistant (MIC, ≥16.0 mg/liter) B. licheniformis strains and one erythromycin-sensitive (MIC, 4.0 mg/liter) B. licheniformis strain. Several amino acid changes were present in the translated ermD and ermK nucleotide sequences of the erythromycin-sensitive strain, which could indicate ErmD and ErmK protein functionalities different from those of the resistance strains. The ermD and ermK genes were localized on an 11.4-kbp plasmid. All of the B. sonorensis strains harbored the bacitracin synthetase gene, bacA, and the transporter gene bcrA, which correlated with their observed resistance to bacitracin. Bacitracin was produced by all the investigated species strains (28%), as determined by ultra-high-definition quadrupole time-of-flight liquid chromatography-mass spectrometry (UHD-QTOF LC/MS). The present study has revealed species-specific variations in the antimicrobial susceptibilities of Bacillus spp. and provides new information on MIC values, as well as the occurrence of resistance genes in Bacillus spp., including the newly described species B. sonorensis. PMID:22941078

  4. The Role of Drosophila Merlin in the Control of Mitosis Exit and Development

    Science.gov (United States)

    2008-07-01

    genome.wus erm-like Brugia malayi merlin-like 316.m00022 http://www.tigr.or Schistosoma japonicum JF2 AAB49033 http://www.nhm.a Taenia saginata myosin-like...value of 100. Although the ERM-like proteins have been identified in Taenia saginata , Schistosoma japonicum, Echinococcus granu- losus, and...like proteins of parasites Taenia saginata , Echinococcus granulo- sus, and Echinococcus multilocularis contain an Arg76 resi- due, which is also a basic

  5. Global gene expression profiling of PAX-FKHR fusion-positive alveolar and PAX-FKHR fusion-negative embryonal rhabdomyosarcomas.

    Science.gov (United States)

    Laé, M; Ahn, E H; Mercado, G E; Chuai, S; Edgar, M; Pawel, B R; Olshen, A; Barr, F G; Ladanyi, M

    2007-06-01

    Paediatric rhabdomyosarcomas (RMS) are classified into two major subtypes based on histological appearance, embryonal (ERMS) and alveolar (ARMS), but this clinically critical distinction is often difficult on morphological grounds alone. ARMS, the more aggressive subtype, is associated in most cases with unique recurrent translocations fusing the PAX3 or PAX7 transcription factor genes to FKHR. In contrast, ERMS lacks unique genetic alterations. To identify novel diagnostic markers and potential therapeutic targets, we analysed the global gene expression profiles of these two RMS subtypes in 23 ARMS (16 PAX3-FKHR, 7 PAX7-FKHR) and 15 ERMS (all PAX-FKHR-negative) using Affymetrix HG-U133A oligonucleotide arrays. A statistically stringent supervised comparison of the ARMS and ERMS expression profiles revealed 121 genes that were significantly differentially expressed, of which 112 were higher in ARMS, including genes of interest as potential diagnostic markers or therapeutic targets, such as CNR1, PIPOX (sarcosine oxidase), and TFAPbeta. Interestingly, many known or putative downstream targets of PAX3-FKHR were highly overexpressed in ARMS relative to ERMS, including CNR1, DCX, ABAT, ASS, JAKMIP2, DKFZp762M127, and NRCAM. We validated the highly differential expression of five genes, including CNR1, DKFZp762M127, DCX, PIPOX, and FOXF1 in ARMS relative to ERMS by quantitative RT-PCR on an independent set of samples. Finally, we developed a ten-gene microarray-based predictor that distinguished ARMS from ERMS with approximately 95% accuracy both in our data by cross-validation and in an independent validation using a published dataset of 26 samples. The gene expression signature of ARMS provides a source of potential diagnostic markers, therapeutic targets, and PAX-FKHR downstream genes, and can be used to reliably distinguish these sarcomas from ERMS. Copyright 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

  6. Electronic Resources Management System: Recommendation Report 2017

    KAUST Repository

    Ramli, Rindra M.

    2017-05-01

    This recommendation report provides an overview of the selection process for the new Electronic Resources Management System. The library has decided to move away from Innovative Interfaces Millennium ERM module. The library reviewed 3 system as potential replacements namely: Proquest 360 Resource Manager, Ex Libris Alma and Open Source CORAL ERMS. After comparing and trialling the systems, it was decided to go for Proquest 360 Resource Manager.

  7. Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci

    OpenAIRE

    Magi, Gloria; Marini, Emanuela; Facinelli, Bruna

    2015-01-01

    In the present study, we have evaluated the in vitro antibacterial activity of essential oils from Origanum vulgare, Thymus vulgaris, Lavandula angustifolia, Mentha piperita, and Melaleuca alternifolia against 32 erythromycin-resistant [Mininum Inhibitory Concentration (MIC) ≥1 μg/mL; inducible, constitutive, and efflux-mediated resistance phenotype; erm(TR), erm(B), and mef(A) genes] and cell-invasive Group A streptococci (GAS) isolated from children with pharyngotonsillitis in Italy. Over the...

  8. MEKANISME CORPORATE GOVERNANCE, ENTERPRISE RISK MANAGEMENT, DAN NILAI PERUSAHAAN PERBANKAN

    OpenAIRE

    Bestari Dwi Handayani

    2017-01-01

    The purpose of this study was to examine corporate governance mechanism. This study also examines the effect of mediation of ERM on relationships corporate governance mechanism and the value of company. The population of this research is financial sector companies listed on the Indonesia Stock Exchange. Sample selection using purposive sampling. This study uses path analysis in hypothesis testing and Sobel test for testing mediation ERM. The results of this study indicate that there is signif...

  9. Evaluation of inner segment/outer segment junctions in different types of epiretinal membranes

    Directory of Open Access Journals (Sweden)

    Rukiye Aydın

    Full Text Available ABSTRACT Purpose: This study was conducted to evaluate the relationships of inner/outer segment (IS/OS junction disruption, macular thickness, and epiretinal membrane (ERM grade with best-corrected visual acuity (BCVA, as well as the relationship between IS/OS junction disruption and ERM grade. Methods: Fifty-four eyes of 54 patients with different grades of ERM were retrospectively reviewed. Patients were classified into three groups by ERM grade according to retinal striae and vessel distortion: grade/group 1, visible membranes without retinal striae or vessel distortion; grade/group 2, mild to moderate macular striae or vessel straightening; and grade/group 3, moderate to severe striae and vascular straightening. Correlations of BCVA with age, central retinal thickness, ERM grade, and IS/OS disruption as well as of IS/OS disruption, central macular thickness, and BCVA with ERM grade were evaluated. Results: Twenty-nine (53.7% eyes exhibited IS/OS junction disruption. Groups 1 and 2 differed significantly with respect to BCVA (p=0.038, but groups 2 and 3 did not (p=0.070. Central macular thickness was significantly greater in group 2 than in group 1 (p=0.031 and in group 3 than in group 2 (p=0.033. Groups 1 and 2 differed significantly in terms of IS/OS disruption (p=0.000, but groups 2 and 3 did not (p=0.310. Conclusions: The IS/OS junction appears to be disrupted during the early stages of ERM. Grade 3 ERM is associated with a significantly higher incidence of IS/OS disruption.

  10. Notch maintains Drosophila type II neuroblasts by suppressing expression of the Fez transcription factor Earmuff.

    Science.gov (United States)

    Li, Xiaosu; Xie, Yonggang; Zhu, Sijun

    2016-07-15

    Notch signaling is crucial for maintaining neural stem cell (NSC) self-renewal and heterogeneity; however, the underlying mechanism is not well understood. In Drosophila, loss of Notch prematurely terminates the self-renewal of larval type II neuroblasts (NBs, the Drosophila NSCs) and transforms type II NBs into type I NBs. Here, we demonstrate that Notch maintains type II NBs by suppressing the activation of earmuff (erm) by Pointed P1 (PntP1). We show that loss of Notch or components of its canonical pathway leads to PntP1-dependent ectopic Erm expression in type II NBs. Knockdown of Erm significantly rescues the loss-of-Notch phenotypes, and misexpression of Erm phenocopies the loss of Notch. Ectopically expressed Erm promotes the transformation of type II NBs into type I NBs by inhibiting PntP1 function and expression in type II NBs. Our work not only elucidates a key mechanism of Notch-mediated maintenance of type II NB self-renewal and identity, but also reveals a novel function of Erm. © 2016. Published by The Company of Biologists Ltd.

  11. An improved method for measuring mismatch negativity using ensemble empirical mode decomposition.

    Science.gov (United States)

    Hsu, Chun-Hsien; Lee, Chia-Ying; Liang, Wei-Kuang

    2016-05-01

    Mismatch negativity (MMN) is a component of event-related potentials (ERPs). Conventional approaches to measuring MMN include recording a large number of trials (e.g., 1000 trials per participant) and extracting signals within a low frequency band, e.g., between 2Hz and 8Hz. Ensemble empirical mode decomposition (EEMD) is a method to decompose time series data into intrinsic mode functions (IMFs). Each IMF has a dominant frequency. Similar to ERP measurement, averaging IMFs across trials allows measurement of event-related modes (ERMs). This paper demonstrates a protocol that adopts EEMD and Hilbert spectral analyses and uses ERMs to extract MMN-related activity based on electroencephalography data recorded from 18 participants in an MMN paradigm. The effect of deviants was demonstrated by manipulating changes in lexical tones. The mean amplitudes of ERMs revealed a significant effect of lexical tone on MMN. Based on effect size statistics, a significant effect of lexical tone on MMN could be observed using ERM measurements over fewer trials (about 300 trials per participant) in a small sample size (five to six participants). The EEMD method provided ERMs with remarkably high signal-to-noise ratios and yielded a strong effect size. Furthermore, the experimental requirements for recording MMN (i.e., the number of trials and the sample size) could be reduced while using the suggested analytic method. ERMs may be useful for applying the MMN paradigm in clinical populations and children. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Endoplasmic reticulum-mitochondria junction is required for iron homeostasis.

    Science.gov (United States)

    Xue, Yong; Schmollinger, Stefan; Attar, Narsis; Campos, Oscar A; Vogelauer, Maria; Carey, Michael F; Merchant, Sabeeha S; Kurdistani, Siavash K

    2017-08-11

    The endoplasmic reticulum (ER)-mitochondria encounter structure (ERMES) is a protein complex that physically tethers the two organelles to each other and creates the physical basis for communication between them. ERMES functions in lipid exchange between the ER and mitochondria, protein import into mitochondria, and maintenance of mitochondrial morphology and genome. Here, we report that ERMES is also required for iron homeostasis. Loss of ERMES components activates an Aft1-dependent iron deficiency response even in iron-replete conditions, leading to accumulation of excess iron inside the cell. This function is independent of known ERMES roles in calcium regulation, phospholipid biosynthesis, or effects on mitochondrial morphology. A mutation in the vacuolar protein sorting 13 ( VPS13 ) gene that rescues the glycolytic phenotype of ERMES mutants suppresses the iron deficiency response and iron accumulation. Our findings reveal that proper communication between the ER and mitochondria is required for appropriate maintenance of cellular iron levels. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  13. Enterprise risk management and firm value within China’s insurance industry

    Directory of Open Access Journals (Sweden)

    Qiuying Li

    2014-02-01

    Research purpose: The purpose of the study is to empirically examine the relationship between ERM and firm value. The study is undertaken within the context of the Chinese insurance industry. Motivation for the study: Recent attempts to link ERM with firm value have been undertaken primarily in the USA and Europe and have produced ambiguous and inconclusive findings. Research design, approach and method: Data was obtained from the China Insurance Regulatory Commission, a government body responsible for regulating insurance products and services in China. The data sample consisted of 135 insurance companies operating in China (in 2010. Regression modelling is employed to analyse the data. Main findings: The results show the relationship between ERM and firm value at first appears statistically significant within a Pearson correlation matrix but then falls below statistical significance on closer scrutiny through regression analysis. Accordingly, it is recommended that insurers in China should not look to aggressive investment in ERM as a strategy for producing quick gains in firm value. Practical/managerial implications: Risk managers should plan ERM development from a risk management maturity perspective, which equates the highest level of ERM development with ERM’s capacity to improve firm resilience to the unknown and serve as a mechanism for strategic decision-making. Contribution/value-add: The study employed return on equity as a proxy for firm value, utilising ordinary least squares regression modelling to test propositions of the relationships between variables.

  14. Transferable Antibiotic Resistances in Marketed Edible Grasshoppers (Locusta migratoria migratorioides).

    Science.gov (United States)

    Osimani, Andrea; Garofalo, Cristiana; Aquilanti, Lucia; Milanović, Vesna; Cardinali, Federica; Taccari, Manuela; Pasquini, Marina; Tavoletti, Stefano; Clementi, Francesca

    2017-05-01

    Grasshoppers are the most commonly eaten insects by humans worldwide, as they are rich in proteins and micronutrients. This study aimed to assess the occurrence of transferable antibiotic resistance genes in commercialized edible grasshoppers. To this end, the prevalence of 12 selected genes [aac(6')-Ie aph(2″)-Ia, blaZ, erm(A), erm(B), erm(C), mecA, tet(M), tet(O), tet(S), tet(K), vanA, vanB] coding for resistance to antibiotics conventionally used in clinical practice was determined. The majority of samples were positive for tet(M) (70.0%), tet(K) (83.3%) and blaZ (83.3%). A low percentage of samples were positive for erm(B) (16.7%), erm(C) (26.7%), and aac(6')-Ie aph(2″)-Ia (13.3%), whereas no samples were positive for erm(A), vanA, vanB, tet(O), and mecA. Cluster analysis identified 4 main clusters, allowing a separation of samples on the basis of their country of origin. © 2017 Institute of Food Technologists®.

  15. Selective breeding provides an approach to increase resistance of rainbow trout ( Onchorhynchus mykiss ) to the diseases, enteric redmouth disease, rainbow trout fry syndrome, and viral haemorrhagic septicaemia

    DEFF Research Database (Denmark)

    Henryon, M.; Berg, P.; Olesen, Niels Jørgen

    2005-01-01

    In this study, we reasoned that if we challenged rainbow trout with the causative agents of enteric redmouth disease (ERM), rainbow trout fry syndrome (RTFS), and viral haemorrhagic septicaemia (VHS), we would: 1) detect additive genetic variation for resistance to ERM, RTFS, and VHS; and 2) find......, RTFS, and VHS will be successful, providing a complementary approach to control these diseases. The weak genetic correlations suggest that it should be relatively easy to improve resistance to each of the diseases simultaneously.......In this study, we reasoned that if we challenged rainbow trout with the causative agents of enteric redmouth disease (ERM), rainbow trout fry syndrome (RTFS), and viral haemorrhagic septicaemia (VHS), we would: 1) detect additive genetic variation for resistance to ERM, RTFS, and VHS; and 2) find...... that resistance of the trout to ERM and RTFS are favourably correlated genetically, while resistance to VHS is unfavourably correlated with resistance to ERM and RTFS. We tested these premises by challenging 63 full-sib families of rainbow trout (50 sires, 38 dams) with Yersinia ruckeri, Flavobacterium...

  16. The Mediator complex subunit MED25 is targeted by the N-terminal transactivation domain of the PEA3 group members.

    Science.gov (United States)

    Verger, Alexis; Baert, Jean-Luc; Verreman, Kathye; Dewitte, Frédérique; Ferreira, Elisabeth; Lens, Zoé; de Launoit, Yvan; Villeret, Vincent; Monté, Didier

    2013-05-01

    PEA3, ERM and ER81 belong to the PEA3 subfamily of Ets transcription factors and play important roles in a number of tissue-specific processes. Transcriptional activation by PEA3 subfamily factors requires their characteristic amino-terminal acidic transactivation domain (TAD). However, the cellular targets of this domain remain largely unknown. Using ERM as a prototype, we show that the minimal N-terminal TAD activates transcription by contacting the activator interacting domain (ACID)/Prostate tumor overexpressed protein 1 (PTOV) domain of the Mediator complex subunit MED25. We further show that depletion of MED25 disrupts the association of ERM with the Mediator in vitro. Small interfering RNA-mediated knockdown of MED25 as well as the overexpression of MED25-ACID and MED25-VWA domains efficiently inhibit the transcriptional activity of ERM. Moreover, mutations of amino acid residues that prevent binding of MED25 to ERM strongly reduce transactivation by ERM. Finally we show that siRNA depletion of MED25 diminishes PEA3-driven expression of MMP-1 and Mediator recruitment. In conclusion, this study identifies the PEA3 group members as the first human transcriptional factors that interact with the MED25 ACID/PTOV domain and establishes MED25 as a crucial transducer of their transactivation potential.

  17. Functional and Molecular Characterization of Ex Vivo Cultured Epiretinal Membrane Cells from Human Proliferative Diabetic Retinopathy

    Directory of Open Access Journals (Sweden)

    Zoltán Veréb

    2013-01-01

    Full Text Available Characterization of the cell surface marker phenotype of ex vivo cultured cells growing out of human fibrovascular epiretinal membranes (fvERMs from proliferative diabetic retinopathy (PDR can give insight into their function in immunity, angiogenesis, and retinal detachment. FvERMs from uneventful vitrectomies due to PDR were cultured adherently ex vivo. Surface marker analysis, release of immunity- and angiogenesis-pathway-related factors upon TNFα activation and measurement of the intracellular calcium dynamics upon mechano-stimulation using fluorescent dye Fura-2 were all performed. FvERMs formed proliferating cell monolayers when cultured ex vivo, which were negative for endothelial cell markers (CD31, VEGFR2, partially positive for hematopoietic- (CD34, CD47 and mesenchymal stem cell markers (CD73, CD90/Thy-1, and PDGFRβ, and negative for CD105. CD146/MCAM and CD166/ALCAM, previously unreported in cells from fvERMs, were also expressed. Secretion of 11 angiogenesis-related factors (DPPIV/CD26, EG-VEGF/PK1, ET-1, IGFBP-2 and 3, IL-8/CXCL8, MCP-1/CCL2, MMP-9, PTX3/TSG-14, Serpin E1/PAI-1, Serpin F1/PEDF, TIMP-1, and TSP-1 were detected upon TNFα activation of fvERM cells. Mechano-stimulation of these cells induced intracellular calcium propagation representing functional viability and role of these cells in tractional retinal detachment, thus serving as a model for studying tractional forces present in fvERMs in PDR ex vivo.

  18. MEKANISME CORPORATE GOVERNANCE, ENTERPRISE RISK MANAGEMENT, DAN NILAI PERUSAHAAN PERBANKAN

    Directory of Open Access Journals (Sweden)

    Bestari Dwi Handayani

    2017-05-01

    Full Text Available The purpose of this study was to examine corporate governance mechanism. This study also examines the effect of mediation of ERM on relationships corporate governance mechanism and the value of company. The population of this research is financial sector companies listed on the Indonesia Stock Exchange. Sample selection using purposive sampling. This study uses path analysis in hypothesis testing and Sobel test for testing mediation ERM. The results of this study indicate that there is significant influence between corporate governance mechanisms (managerial ownership, institutional ownership, independent commissioner and audit committee with the value of company. This study provides evidence that ERM mediating influence between institutional ownership, independent directors and audit committee with company’s value, but not significant in mediating the effect of managerial ownership. Penelitian ini bertujuan untuk menguji mekanisme corporate governance. Penelitian ini juga menguji pengaruh mediasi dari ERM pada hubungan mekanisme corporate governance dan nilai perusahaan tersebut. Populasi penelitian ini adalah perusahaan sektor keuangan yang terdaftar di Bursa Efek Indonesia (BEI. Teknik pemilihan sampel menggunakan purposive sampling. Penelitian ini menggunakan analisis jalur (path analysis dalam pengujian hipotesis dan sobel test untuk pengujian mediasi ERM. Hasil penelitian ini menunjukkan bahwa terdapat pengaruh signifikan antara mekanisme corporate governance (kepemilikan manajerial, kepemilikan institusional, komisaris independen, dan komite audit dengan nilai perusahaan. Penelitian ini memberikan bukti bahwa ERM memediasi pengaruh antara kepemilikan institusional, komisaris independen, dan komite audit terhadap nilai perusahaan. Namun tidak signifikan dalam memediasi pengaruh kepemilikan manajerial.

  19. Enterprise Risk Management Implementation Maturity in Financial Companies

    Directory of Open Access Journals (Sweden)

    Subagio Tjahjono

    2017-07-01

    Full Text Available Previous studies of ERM implementation mostly using dummy variable to figure out relation with its determinant. Until today, studies that using risk management maturity as real variable are limited. Therefore, this study intends to determine the maturity level of ERM implementation in non-financial companies listed on Indonesia Stock Exchange during period 2015 and influence of firm’s total assets, total employee, leverage and public ownership to the ERM maturity. The method use are qualitative and multiple regression analysis. The result of data analysis showed that ERM implementation in selected sample during 2015 are still low (majority in initial and repeatable level. In addition, from determinant factors only total asset that have significant influence to the ERM maturity. This result implies that non-financial listed companies are vulnerable to risks. Management should consider future benefit of a mature ERM, not only to comply with regulation.DOI: 10.15408/etk.v16i2.5440 

  20. Managers’ Support – A Key Driver behind Enterprise Risk Management Maturity

    Directory of Open Access Journals (Sweden)

    Sprčić Danijela Miloš

    2017-04-01

    Full Text Available Severe consequences of the global fi nancial crisis resulted in re-thinking the risk management processes and approaches, highlighting the need for a comprehensive risk management framework. Consequently, more and more companies are moving away from the Traditional “silo-based” Risk Management (TRM to a more holistic approach known as Enterprise Risk Management (ERM. This paper presents results of both exploratory and empirical research. First, we develop ERM Index that measures maturity of ERM process within the company. Then, we present empirical results on the level of maturity and determinants of risk management system development in listed Croatian companies. Research indicates low levels of ERM development: even 38 per cent of analysed companies have no elements of ERM system, from which 22 per cent do not manage corporate risks at all. Except the company’s size supported by the economies of scale argument, managers’ support is the most important determinant of ERM system maturity in Croatian companies.

  1. PENGARUH PENGUNGKAPAN ENTERPRISE RISK MANAGEMENT DAN PENGUNGKAPAN INTELLECTUAL CAPITAL TERHADAP NILAI PERUSAHAAN

    Directory of Open Access Journals (Sweden)

    Sunitha Devi

    2017-06-01

    Full Text Available This research aims to find out empirical evidences on the effect of ERM disclosure and IC disclosure on firm value. The sample of the research is 73 non-financial companies listed in Indonesia Stock Exchange for the period of 2010-2014. Regression analysis of panel data was applied to analyze the data. The results show that ERM disclosure has positive and significant effect on the firm value. This study also finds that IC disclosure has positive and significant effect on the firm value. Firm size, profitability, and leverage, as control variables, are also contributing positive and significant effects on the firm value. The results of this research can be used as a consideration for the company’s management to increase ERM and IC disclosures in the annual report as ERM and IC disclosures can be a positive signal to encourage the increase in corporate value. In addition, since ERM and IC information are very significant for investors, it can also be beneficial for the regulator to set up and establish instruments of mandatory disclosure related to ERM and IC to minimize the asymmetry of information which can disadvantage company’s related parties.

  2. Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil.

    Science.gov (United States)

    Pereira, Jussyêgles Niedja da Paz; Rabelo, Marcelle Aquino; Lima, Jailton Lobo da Costa; Neto, Armando Monteiro Bezerra; Lopes, Ana Catarina de Souza; Maciel, Maria Amélia Vieira

    2016-01-01

    There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail. To characterize the phenotypic (occurrence of cMLSB and iMLSB phenotypes) and molecular (occurrence of ermA and ermC genes) profiles of MLSB resistance of clinical isolates of susceptible and methicillin-resistant Staphylococcus aureus and CNS (coagulase-negative Staphylococcus) from patients of a university hospital, in Pernambuco. The antimicrobial susceptibility of 103 isolates was determined by the disk diffusion technique in Mueller-Hinton agar followed by oxacillin screening. The iMLSB phenotype was detected by D test. Isolates with cMLSB and iMLSB phenotypes were subjected to polymerase chain reaction (PCR) for the detection of ermA and ermC genes. The cMLSB and iMLSB phenotypes were respectively identified in 39 (37.9%) and five (4.9%) isolates. The iMLSB phenotype was found only in four (10.8%) methicillin-susceptible S. aureus and one (4.5%) methicillin-resistant S. aureus. In the 44 isolates subjected to PCR, four (9.1%) only ermA gene was detected, a lower frequency when compared to only ermC 17 (38.6%) gene and to one (2.3%) isolate presenting both genes. In the Staphylococcus spp. analyzed, the ermC gene was found more often than the ermA, although the iMLSB phenotype had been less frequent than the cMLSB. It was important to perform the D test for its detection to guide therapeutic approaches. Copyright © 2016 Elsevier Editora Ltda. All rights reserved.

  3. Enterprise risk management and firm value within China’s insurance industry

    Directory of Open Access Journals (Sweden)

    Qiuying Li

    2014-02-01

    Full Text Available Orientation: The article discusses the relationship between enterprise risk management (ERM and firm value.Research purpose: The purpose of the study is to empirically examine the relationship between ERM and firm value. The study is undertaken within the context of the Chinese insurance industry.Motivation for the study: Recent attempts to link ERM with firm value have been undertaken primarily in the USA and Europe and have produced ambiguous and inconclusive findings.Research design, approach and method: Data was obtained from the China Insurance Regulatory Commission, a government body responsible for regulating insurance products and services in China. The data sample consisted of 135 insurance companies operating in China (in 2010. Regression modelling is employed to analyse the data.Main findings: The results show the relationship between ERM and firm value at first appears statistically significant within a Pearson correlation matrix but then falls below statistical significance on closer scrutiny through regression analysis. Accordingly, it is recommended that insurers in China should not look to aggressive investment in ERM as a strategy for producing quick gains in firm value.Practical/managerial implications: Risk managers should plan ERM development from a risk management maturity perspective, which equates the highest level of ERM development with ERM’s capacity to improve firm resilience to the unknown and serve as a mechanism for strategic decision-making.Contribution/value-add: The study employed return on equity as a proxy for firm value, utilising ordinary least squares regression modelling to test propositions of the relationships between variables. Normal 0 false false false EN-GB ZH-CN AR-SA Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

    Directory of Open Access Journals (Sweden)

    Chirles A. França

    2012-08-01

    Full Text Available The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210 isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efflux pump test was performed using ethidium bromide and biofilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%, streptomycin (42.8%, tetracycline (40.4%, lincomycin (39.0% and erythromycin (33.8%. Pan-susceptibility to all tested drugs was observed in 71 (33.8% isolates and 41 Staphylococcus isolates were positive for the efflux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efflux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.

  4. Pharmacological targeting of the ephrin receptor kinase signalling by GLPG1790 in vitro and in vivo reverts oncophenotype, induces myogenic differentiation and radiosensitizes embryonal rhabdomyosarcoma cells

    Directory of Open Access Journals (Sweden)

    Francesca Megiorni

    2017-10-01

    Full Text Available Abstract Background EPH (erythropoietin-producing hepatocellular receptors are clinically relevant targets in several malignancies. This report describes the effects of GLPG1790, a new potent pan-EPH inhibitor, in human embryonal rhabdomyosarcoma (ERMS cell lines. Methods EPH-A2 and Ephrin-A1 mRNA expression was quantified by real-time PCR in 14 ERMS tumour samples and in normal skeletal muscle (NSM. GLPG1790 effects were tested in RD and TE671 cell lines, two in vitro models of ERMS, by performing flow cytometry analysis, Western blotting and immunofluorescence experiments. RNA interfering experiments were performed to assess the role of specific EPH receptors. Radiations were delivered using an x-6 MV photon linear accelerator. GLPG1790 (30 mg/kg in vivo activity alone or in combination with irradiation (2 Gy was determined in murine xenografts. Results Our study showed, for the first time, a significant upregulation of EPH-A2 receptor and Ephrin-A1 ligand in ERMS primary biopsies in comparison to NSM. GLPG1790 in vitro induced G1-growth arrest as demonstrated by Rb, Cyclin A and Cyclin B1 decrease, as well as by p21 and p27 increment. GLPG1790 reduced migratory capacity and clonogenic potential of ERMS cells, prevented rhabdosphere formation and downregulated CD133, CXCR4 and Nanog stem cell markers. Drug treatment committed ERMS cells towards skeletal muscle differentiation by inducing a myogenic-like phenotype and increasing MYOD1, Myogenin and MyHC levels. Furthermore, GLPG1790 significantly radiosensitized ERMS cells by impairing the DNA double-strand break repair pathway. Silencing of both EPH-A2 and EPH-B2, two receptors preferentially targeted by GLPG1790, closely matched the effects of the EPH pharmacological inhibition. GLPG1790 and radiation combined treatments reduced tumour mass by 83% in mouse TE671 xenografts. Conclusions Taken together, our data suggest that altered EPH signalling plays a key role in ERMS development and that

  5. Measurement of Missing Transverse Energy With the CMS Detector at the LHC

    CERN Document Server

    Pi, Haifeng; Green, Dan; Rohlf, James; Tully, Christopher

    2006-01-01

    The performance of the Compact Muon Solenoid detector for measuring missing transverse energy is evaluated using fully simulated pp collisions at a center-of-mass energy of 14 TeV at the Large Hadron Collider. For minimum bias events without pileup, a resolution of 6.1 GeV is computed, corresponding to a stochastic contribution of $0.63 \\sqrt{\\Sigma E_{\\rm T}}~{\\rm GeV}^{1/2}$, where $\\Sigma E_{\\rm T}$ is the summed transverse energy in all calorimeter towers. When the contribution of pileup is included, the resolution degrades according to the overall deposited $\\Sigma E_{\\rm T}$ with the same stochastic coefficient. For QCD dijet events with event pileup corresponding to a luminosity ${\\cal L} = 2\\times 10^{33}{\\rm~cm}^{-2} {\\rm s}^{-1}$, we compute $\\sigma= [(3.8 {\\rm ~GeV})^2+(0.97 {\\rm ~GeV}^{1/2}\\sqrt{\\Sigma E_{\\rm T}})^2+ (0.012\\Sigma E_{\\rm T})^2]^{1/2}$ resulting in a resolution of 45 GeV for jet events with reconstructed transverse momentum of 800 GeV/$c$. Monte Carlo samples of ${\\rm t}{\\rm \\bar t}...

  6. Parallelism as a novel marker for structural integrity of retinal layers in optical coherence tomographic images in eyes with epiretinal membrane.

    Science.gov (United States)

    Uji, Akihito; Murakami, Tomoaki; Unoki, Noriyuki; Ogino, Ken; Nishijima, Kazuaki; Yoshitake, Shin; Dodo, Yoko; Yoshimura, Nagahisa

    2014-01-01

    To propose a new parameter, "Parallelism," to evaluate retinal layer integrity on spectral-domain optical coherence tomography (SDOCT), and to investigate the association between parallelism and visual function in eyes with idiopathic epiretinal membrane (ERM). Retrospective, observational evaluation of a diagnostic test. We evaluated a consecutive series of 57 eyes of 57 patients with ERM and 30 healthy eyes of 30 volunteers for whom M-CHARTS testing and SDOCT were performed on the same day. OCT images were skeletonized, and the orientation of segmented lines in the image was termed "Parallelism" and was expressed as a value ranging from 0-1 and increasing as the retinal layers ran more parallel with each other. The relationships between parallelism and visual acuity and between parallelism and metamorphopsia score were evaluated. In normal eyes, parallelism was nearly homogeneous and varied slightly with the location. Parallelism in eyes with ERM was significantly lower than that in normal eyes. In the horizontal and vertical scans, parallelism was significantly correlated with visual acuity, horizontal metamorphopsia score, and vertical metamorphopsia score. Parallelism of the center (1 mm) in the horizontal scan was strongly correlated with horizontal metamorphopsia score (R = -0.632; P parallelism and retinal thickness both in horizontal and vertical scans. Parallelism was significantly lower in eyes with ERM than in normal eyes, and correlated strongly with metamorphopsia and visual acuity in eyes with ERM. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. The NHE1 Na+/H+ exchanger recruits ezrin/radixin/moesin proteins to regulate Akt-dependent cell survival.

    Science.gov (United States)

    Wu, Karen L; Khan, Shenaz; Lakhe-Reddy, Sujata; Jarad, George; Mukherjee, Amitava; Obejero-Paz, Carlos A; Konieczkowski, Martha; Sedor, John R; Schelling, Jeffrey R

    2004-06-18

    Apoptosis results in cell shrinkage and intracellular acidification, processes opposed by the ubiquitously expressed NHE1 Na(+)/H(+) exchanger. In addition to mediating Na(+)/H(+) transport, NHE1 interacts with ezrin/radixin/moesin (ERM), which tethers NHE1 to cortical actin cytoskeleton to regulate cell shape, adhesion, motility, and resistance to apoptosis. We hypothesize that apoptotic stress activates NHE1-dependent Na(+)/H(+) exchange, and NHE1-ERM interaction is required for cell survival signaling. Apoptotic stimuli induced NHE1-regulated Na(+)/H(+) transport, as demonstrated by ethyl-N-isopropyl-amiloride-inhibitable, intracellular alkalinization. Ectopic NHE1, but not NHE3, expression rescued NHE1-null cells from apoptosis induced by staurosporine or N-ethylmaleimide-stimulated KCl efflux. When cells were subjected to apoptotic stress, NHE1 and phosphorylated ERM physically associated within the cytoskeleton-enriched fraction, resulting in activation of the pro-survival kinase, Akt. NHE1-associated Akt activity and cell survival were inhibited in cells expressing ERM binding-deficient NHE1, dominant negative ezrin constructs, or ezrin mutants with defective binding to phosphoinositide 3-kinase, an upstream regulator of Akt. We conclude that NHE1 promotes cell survival by dual mechanisms: by defending cell volume and pH(i) through Na(+)/H(+) exchange and by functioning as a scaffold for recruitment of a signalplex that includes ERM, phosphoinositide 3-kinase, and Akt.

  8. Oncogenic NRAS, Required for Pathogenesis of Embryonic Rhabdomyosarcoma, Relies upon the HMGA2–IGF2BP2 Pathway

    Science.gov (United States)

    Li, Zhizhong; Zhang, Yunyu; Ramanujan, Krishnan; Ma, Yan; Kirsch, David G.; Glass, David J.

    2013-01-01

    Embryonic rhabdomyosarcoma (ERMS) is the most common soft-tissue tumor in children. Here, we report the identification of the minor groove DNA-binding factor high mobility group AT-hook 2 (HMGA2) as a driver of ERMS development. HMGA2 was highly expressed in normal myoblasts and ERMS cells, where its expression was essential to maintain cell proliferation, survival in vitro, and tumor outgrowth in vivo. Mechanistic investigations revealed that upregulation of the insulin–like growth factor (IGF) mRNA-binding protein IGF2BP2 was critical for HMGA2 action. In particular, IGF2BP2 was essential for mRNA and protein stability of NRAS, a frequently mutated gene in ERMS. shRNA-mediated attenuation of NRAS or pharmacologic inhibition of the MAP-ERK kinase (MEK)/extracellular signal-regulated kinase (ERK) effector pathway showed that NRAS and NRAS-mediated signaling was required for tumor maintenance. Taken together, these findings implicate the HMGA2–IGFBP2–NRAS signaling pathway as a critical oncogenic driver in ERMS. PMID:23536553

  9. Oncogenic NRAS, required for pathogenesis of embryonic rhabdomyosarcoma, relies upon the HMGA2-IGF2BP2 pathway.

    Science.gov (United States)

    Li, Zhizhong; Zhang, Yunyu; Ramanujan, Krishnan; Ma, Yan; Kirsch, David G; Glass, David J

    2013-05-15

    Embryonic rhabdomyosarcoma (ERMS) is the most common soft-tissue tumor in children. Here, we report the identification of the minor groove DNA-binding factor high mobility group AT-hook 2 (HMGA2) as a driver of ERMS development. HMGA2 was highly expressed in normal myoblasts and ERMS cells, where its expression was essential to maintain cell proliferation, survival in vitro, and tumor outgrowth in vivo. Mechanistic investigations revealed that upregulation of the insulin-like growth factor (IGF) mRNA-binding protein IGF2BP2 was critical for HMGA2 action. In particular, IGF2BP2 was essential for mRNA and protein stability of NRAS, a frequently mutated gene in ERMS. shRNA-mediated attenuation of NRAS or pharmacologic inhibition of the MAP-ERK kinase (MEK)/extracellular signal-regulated kinase (ERK) effector pathway showed that NRAS and NRAS-mediated signaling was required for tumor maintenance. Taken together, these findings implicate the HMGA2-IGFBP2-NRAS signaling pathway as a critical oncogenic driver in ERMS. ©2013 AACR.

  10. VISUALIZATION OF MACULAR PUCKER BY MULTICOLOR SCANNING LASER IMAGING.

    Science.gov (United States)

    Kilic Muftuoglu, Ilkay; Bartsch, Dirk-Uwe; Barteselli, Giulio; Gaber, Raouf; Nezgoda, Joseph; Freeman, William R

    2018-02-01

    To compare the visualization of the epiretinal membrane (ERM) using multicolor imaging (MCI) (Heidelberg Engineering, Carlsbad, CA) and conventional white light flood color fundus photography (FP) (Topcon). The paired images of patients with ERM who underwent same-day MCI and FP examinations were reviewed. Visibility of the ERM was graded using a scale (0: not visible, 1: barely visible, and 2: clearly visible) by masked readers, and surface folds were counted to quantify the membrane visibility for each method. Images from individual color channels in MCI (green, blue, and infrared) were also graded using the same method to further investigate MCI images. Forty-eight eyes of 42 patients were included. The average ERM visibility score was 1.8 ± 0.37 for MCI and 1.01 ± 0.63 for FP (P provided better detection of surface folds (5.54 ± 2.12) compared to blue reflectance (4.2 ± 2.34) and infrared reflectance (1.2 ± 0.9). Multicolor scanning laser imaging provides superior ERM detection and delineation of surface folds than conventional FP, primarily due to the green channel present in the combination-pseudocolor image in MCI.

  11. Identification and characterization of proliferative retinopathy-related long noncoding RNAs

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, Rong-Mei; Wang, Xiao-Qun; Yao, Jin [Eye Hospital, Nanjing Medical University, Nanjing (China); The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing (China); Shen, Yi; Chen, Sai-Nan [Eye Hospital, Nanjing Medical University, Nanjing (China); Yang, Hong [Eye Hospital, Nanjing Medical University, Nanjing (China); The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing (China); Jiang, Qin, E-mail: jqin710@vip.sina.com [Eye Hospital, Nanjing Medical University, Nanjing (China); The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing (China); Institute of Integrated Medicine, Nanjing Medical University, Nanjing (China); Yan, Biao, E-mail: yanbiao1982@hotmail.com [Eye Hospital, Nanjing Medical University, Nanjing (China); The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing (China); Institute of Integrated Medicine, Nanjing Medical University, Nanjing (China)

    2015-09-25

    Proliferative vitreoretinopathy (PVR) is a serious complication of retinal detachment and vitreoretinal surgery, which can lead to severe vision reduction. Long non-coding RNAs (lncRNAs) play critical roles in many biological processes and disease development. We attempted to determine the role of lncRNAs in the setting of PVR. Microarray analysis revealed that 78 lncRNAs were abnormally expressed in the epiretinal membranes (ERMs) of PVR patients, including 48 up-regulated and 30 down-regulated lncRNA transcripts. We subsequently focus on one lncRNA, MALAT1, and investigated its expression pattern in the biofluid of PVR patients. MALAT1 was significantly up-regulated in the cellular and plasma fraction of peripheral blood in PVR patients. MALAT1 expression was obviously reduced after PVR operation. In vitro experiments revealed the role of MALAT1 in regulating RPE proliferation and migration, which is critical for ERMs formation. This study suggests that lncRNAs are the potential regulators of PVR pathology. MALAT1 is a potential prognostic indicator and a target for the diagnosis and gene therapy for PVR diseases. - Highlights: • 78 lncRNAs are differentially expressed between PVR-ERMs and secondary ERMs. • MALAT1 level is elevated in the ERMs of PVR patients. • Circulating MALAT1 level is up-regulated in PVR patients. • MALAT1 knockdown regulates RPE proliferation and migration.

  12. Antimicrobial resistance in Danish pigs: A cross sectional study of the association between antimicrobial resistance and geography, exposure to antimicrobials, and trade

    DEFF Research Database (Denmark)

    Birkegård, Anna Camilla

    was on seven antimicrobial resistance genes - ermB, ermF, sulI, sulII, tet(M), tet(O), and tet(W). The levels of these genes were determined using a quantitative real-time polymerase chain reaction (qPCR). The erm genes confer resistance against macrolides, the sul genes confer resistance against sulfonamides...... excluded. The samples were collected at five abattoirs located on Zealand and in Jutland. The sample material was faeces, and it was collected by squeezing a small amount out of the rectum at the slaughter line. After collection the samples were sent to the laboratory. The samples were pooled into one...... aliquot per farm and DNA was extracted from the faecal material. The antimicrobial resistance gene levels were then measured using quantitative real-time PCR. As the samples were collected at the abattoir it was not possible to know in advance which farmswere available for sampling. Consequently...

  13. Prototypic Enhanced Risk Monitor Framework and Evaluation - Advanced Reactor Technology Milestone: M3AT-15PN2301054

    Energy Technology Data Exchange (ETDEWEB)

    Ramuhalli, Pradeep [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hirt, Evelyn H. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Veeramany, Arun [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Bonebrake, Christopher A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ivans, William J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Coles, Garill A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Coble, Jamie B. [Univ. of Tennessee, Knoxville, TN (United States); Liu, X. [Univ. of Tennessee, Knoxville, TN (United States); Wootan, David W. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mitchell, Mark R. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Brass, Mary F. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-09-24

    This research report summaries the development and evaluation of a prototypic enhanced risk monitor (ERM) methodology (framework) that includes alternative risk metrics and uncertainty analysis. This updated ERM methodology accounts for uncertainty in the equipment condition assessment (ECA), the prognostic result, and the probabilistic risk assessment (PRA) model. It is anticipated that the ability to characterize uncertainty in the estimated risk and update the risk estimates in real time based on equipment condition assessment (ECA) will provide a mechanism for optimizing plant performance while staying within specified safety margins. These results (based on impacting active component O&M using real-time equipment condition information) are a step towards ERMs that, if integrated with AR supervisory plant control systems, can help control O&M costs and improve affordability of advanced reactors.

  14. Vps13-Mcp1 interact at vacuole-mitochondria interfaces and bypass ER-mitochondria contact sites.

    Science.gov (United States)

    John Peter, Arun T; Herrmann, Beatrice; Antunes, Diana; Rapaport, Doron; Dimmer, Kai Stefan; Kornmann, Benoît

    2017-10-02

    Membrane contact sites between endoplasmic reticulum (ER) and mitochondria, mediated by the ER-mitochondria encounter structure (ERMES) complex, are critical for mitochondrial homeostasis and cell growth. Defects in ERMES can, however, be bypassed by point mutations in the endosomal protein Vps13 or by overexpression of the mitochondrial protein Mcp1. How this bypass operates remains unclear. Here we show that the mitochondrial outer membrane protein Mcp1 functions in the same pathway as Vps13 by recruiting it to mitochondria and promoting its association to vacuole-mitochondria contacts. Our findings support a model in which Mcp1 and Vps13 work as functional effectors of vacuole-mitochondria contact sites, while tethering is mediated by other factors, including Vps39. Tethered and functionally active vacuole-mitochondria interfaces then compensate for the loss of ERMES-mediated ER-mitochondria contact sites. © 2017 John Peter et al.

  15. Precise orbit computation for the Geosat Exact Repeat Mission

    Science.gov (United States)

    Haines, Bruce J.; Born, George H.; Rosborough, George W.; Marsh, James G.; Williamson, Ronald G.

    1990-03-01

    Results are reported from an extensive investigation of orbit-determination strategies for the Geosat Exact Repeat Mission (ERM). The goal is to establish optimum geodetic parameters and procedures for the computation of the most accurate Geosat orbits possible and to apply these procedures for routine computation during the ERM for the following purposes: (1) to enhance the value of the Geosat oceanographic investigations by providing the user community with improved ephemerides, (2) to develop orbit determination techniques for the upcoming altimetric mission Topex/Poseidon, and (3) to assess the radial orbit accuracy obtainable with recently developed gravity models. To this end, ephemerides for the entire first year of the ERM have been computed using the GEODYN II orbit program on the Cyber 205 supercomputer system at the NASA Goddard.

  16. Resistance of potential probiotic lactic acid bacteria and bifidobacteria of African and European origin to antimicrobials: Determination and transferability of the resistance genes to other bacteria

    DEFF Research Database (Denmark)

    Ouoba, Labia Irene Ivette; Lei, Vicki; Jensen, Lars Bogø

    2008-01-01

    antimicrobials, detection of resistance genes by PCR reactions using specific primers and sequencing of positive amplicons. The ability of Lb. reuteri from Africa to transfer the erythromycin resistance gene erm(B) to closely related bacteria was investigated by conjugation. Variations were observed and high...... found in Lb. reuteri L4: 12002 from Africa contained a homologous sequence to previously published sequences. This gene could be transferred in vitro to enterococci. Higher prevalence of phenotypic resistance for aminoglycoside was found in isolates from Europe. (C) 2007 Elsevier B.V. All rights...... levels of intrinsic resistance were found among the tested species. Positive amplicons were obtained for resistance genes encoding aminoglycoside (aph(3')-III, aadA, aadE) and tetracycline (tet(S)) from isolates from Europe and macrolide (erm(B)) from an isolate from Africa. However, only the erm(B) gene...

  17. Epiretinal membrane surgery

    DEFF Research Database (Denmark)

    Hamoudi, Hassan; Correll Christensen, Ulrik; La Cour, Morten

    2017-01-01

    Purpose: To assess the impact of combined phacoemulsification-vitrectomy and sequential surgery for idiopathic epiretinal membrane (ERM) on refractive error (RE) and macular morphology. Methods: In this prospective clinical trial, we allocated phakic eyes with ERM to (1) cataract surgery...... between the groups. Four cases (17%) in the CAT group had resolved visual complaints and improved BCVA after cataract surgery resulting in no need for PPV within the follow-up period. Conclusion: Surgery for idiopathic ERM in phakic eyes with either phaco-vitrectomy or sequential surgery are equal...... and subsequent pars plana vitrectomy (PPV) (CAT group), (2) PPV and subsequent cataract surgery (VIT group) or (3) phaco-vitrectomy (COMBI group). Examinations were at baseline, one month after each surgery, and at 3 months and 12 months of follow-up. Primary outcome was the RE (the difference between predicted...

  18. Investigation of an experimental ejector refrigeration machine operating with refrigerant R245fa at design and off-design working conditions. Part 1. Theoretical analysis

    KAUST Repository

    Shestopalov, K.O.

    2015-07-01

    © 2015 Elsevier Ltd and IIR.All rights reserved. The ejector refrigeration machine (ERM) offers several advantages over other heat-driven refrigeration machine, including simplicity in design and operation, high reliability and low installation cost, which enable its wide application in the production of cooling. In this paper the theoretical analysis of ejector design and ejector refrigeration cycle performance is presented. It is shown that ERM performance characteristics depend strongly on the operating conditions, the efficiency of the ejector used, and the thermodynamic properties of the refrigerant used. A 1-D model for the prediction of the entrainment ratio ω, and an optimal design for ejectors with cylindrical and conical-cylindrical mixing chambers are presented in this paper. In order to increase ERM performance values, it is necessary first of all to improve the performance of the ejector.

  19. Pursuing enterprise risk management: a local road map for Canadian healthcare leaders.

    Science.gov (United States)

    Haney, James R; Church, John; Cockerill, Rhonda

    2013-01-01

    An in-depth analysis of organizational risk management in healthcare, and in particular the concepts of Enterprise Risk Management (ERM), has identified a 5-part model that can be used by Canadian healthcare leaders as an evidence-supported approach to successful organizational risk management. The Model for Organizational Risk Management, termed "the Model," has been developed as a basis for linking the components of an ERM Framework into a Canadian health organization to overcome the barriers that commonly disrupt strategic risk management. The Model addresses how an ERM Framework can fit within an existing health organization by building off and enhancing existing processes and resources to ensure familiarity, acceptance, and sustainability of the risk management program. By approaching the Model in a stepwise fashion (based on individual organizational context), healthcare leaders are provided with a road map from which to advance their own organizational risk management program.

  1. Speak up! Enhancing risk performance with enterprise risk management, leadership style and employee voice

    DEFF Research Database (Denmark)

    Sax, Johanna; Torp, Simon

    2015-01-01

    Purpose: – The purpose of this paper is to test the effect of psychological safety and participative leadership style on risk performance as well as its interaction with enterprise risk management (ERM) processes to evaluate if a decentralisation in the form of a safe environment and participative......: – The paper finds that not only do both ERM and participative leadership style enhance risk performance but a positive interaction effect is also found. In addition, the findings suggest that a safe environment precede participative leadership style indicating this as a prerequisite for management...... to introduce participative leadership style. These findings underpin that an effective risk management system should include both a holistic, formalised ERM system and organisational initiatives that enhance a strategic responsiveness through employee involvement. Originality/value: – The current study...

  2. Antibiotic susceptibility pattern and erythromycin resistance mechanisms in beta-hemolytic group G Streptococcus dysgalactiae subspecies equisimilis isolates from central Taiwan.

    Science.gov (United States)

    Lo, Hsueh-Hsia; Nien, Hao-Hsiang; Cheng, Ya-Yu; Su, Fang-Yi

    2015-12-01

    Information concerning antibiotics susceptibilities of beta-hemolytic group G Streptococcus dysgalactiae subspecies equisimilis (SDSE) clinical isolates in central Taiwan was limited. Totally, 246 SDSE isolates were collected from mainly five regional hospitals, from February 2007 to August 2011. Disk diffusion method, broth microdilution method, and clindamycin induction test (D test) were respectively performed according to the guidelines of the Clinical and Laboratory Standards Institute. Polymerase chain reaction was used to detect the corresponding erythromycin resistance genes. All isolates were susceptible to penicillin, cefotaxime, and vancomycin. The rate of erythromycin resistance was 24.0% (59/246), whereas that of clindamycin resistance was 12.2% (30/246). The resistance rates of isolates from different hospitals varied from 15.0% to 45.5% for erythromycin and from 7.1% to 36.4% for clindamycin. For erythromycin-resistant SDSE isolates, three different phenotypes with resistance to macrolides (M), lincosamides (L), and type B streptogramins (SB) were observed: M (49.2%), constitutive MLSB (cMLSB, 35.6%), and inducible MLSB (iMLSB, 15.3%). All M phenotypic isolates carried mefA. The most prevalent genotypes among cMLSB and iMLSB phenotypic isolates were ermB, followed by ermTR. One isolate with cMLSB phenotype carried both ermB and ermTR, whereas one isolate with iMLSB phenotype carried both ermB and ermC. This is the first trial investigating the antimicrobial susceptibility pattern and erythromycin resistance mechanisms of beta-hemolytic group G SDSE isolates in central Taiwan. The resistance rates for both erythromycin and clindamycin varied significantly among hospitals located in this area and should be monitored continuously in the future. Copyright © 2014. Published by Elsevier B.V.

  3. Possible Origins of CTnBST, a Conjugative Transposon Found Recently in a Human Colonic Bacteroides Strain▿

    Science.gov (United States)

    Schlesinger, David J.; Shoemaker, Nadja B.; Salyers, Abigail A.

    2007-01-01

    A previous survey of Bacteroides isolates suggested that the ermB gene entered Bacteroides spp. recently. Previously, ermB had been found almost exclusively in gram-positive bacteria. In one Bacteroides strain, ermB was located on 100-kb conjugative transposon (CTn) CTnBST. To assess the possible origin of this CTn, we obtained the full DNA sequence of CTnBST and used this information to investigate its possible origins. Over one-half of CTnBST had high sequence identity to a putative CTn found in the genome of Bacteroides fragilis YCH46. This included the ends of the CTn and genes involved in integration, transfer, and excision. However, the region around the ermB gene contained genes that appeared to originate from gram-positive organisms. In particular, a 7-kb segment containing the ermB gene was 100% identical to an ermB region found in the genome of the gram-positive bacterium Arcanobacterium pyogenes. A screen of Bacteroides isolates whose DNA cross-hybridized with a CTnBST probe revealed that several isolates did not carry the 7-kb region, implying that the acquisition of this region may be more recent than the acquisition of the entire CTnBST element by Bacteroides spp. We have also identified other Bacteroides isolates that carry a slightly modified 7-kb region but have no other traces of CTnBST. Thus, it is possible that this 7-kb region could itself be part of a mobile element that has inserted in a Bacteroides CTn. Our results show that CTnBST is a hybrid element which has acquired a portion of its coding region from gram-positive bacteria but which may originally have come from Bacteroides spp. or some related species. PMID:17483268

  4. Possible origins of CTnBST, a conjugative transposon found recently in a human colonic Bacteroides strain.

    Science.gov (United States)

    Schlesinger, David J; Shoemaker, Nadja B; Salyers, Abigail A

    2007-07-01

    A previous survey of Bacteroides isolates suggested that the ermB gene entered Bacteroides spp. recently. Previously, ermB had been found almost exclusively in gram-positive bacteria. In one Bacteroides strain, ermB was located on 100-kb conjugative transposon (CTn) CTnBST. To assess the possible origin of this CTn, we obtained the full DNA sequence of CTnBST and used this information to investigate its possible origins. Over one-half of CTnBST had high sequence identity to a putative CTn found in the genome of Bacteroides fragilis YCH46. This included the ends of the CTn and genes involved in integration, transfer, and excision. However, the region around the ermB gene contained genes that appeared to originate from gram-positive organisms. In particular, a 7-kb segment containing the ermB gene was 100% identical to an ermB region found in the genome of the gram-positive bacterium Arcanobacterium pyogenes. A screen of Bacteroides isolates whose DNA cross-hybridized with a CTnBST probe revealed that several isolates did not carry the 7-kb region, implying that the acquisition of this region may be more recent than the acquisition of the entire CTnBST element by Bacteroides spp. We have also identified other Bacteroides isolates that carry a slightly modified 7-kb region but have no other traces of CTnBST. Thus, it is possible that this 7-kb region could itself be part of a mobile element that has inserted in a Bacteroides CTn. Our results show that CTnBST is a hybrid element which has acquired a portion of its coding region from gram-positive bacteria but which may originally have come from Bacteroides spp. or some related species.

  5. Molecular Level Investigation of Staphylococci’s Resistance Mechanisms to Antibiotics

    Directory of Open Access Journals (Sweden)

    Lavinia Lorena PRUTEANU

    2017-09-01

    Full Text Available Polymerase chain reaction (PCR techniques development allows elaboration of many assays for identification of bacteria’s resistance mechanisms to antibiotics. Following this idea, the results of molecular level investigation of bacteria’s resistance mechanisms to antibiotics may give many opportunities to find more rapid methods for identifying the genes which are responsible for antibiotic resistance induction. The aim of this study was to investigate antibiotic resistance genes in Staphylococcus bacteria on molecular level. As classes of antibiotics it was used macrolides-lincosamides-streptogramin B (MLSB and beta-lactams. In the proposed study the bacterial strains are represented by 50 isolates of Staphylococcus. The bacterial strains were analyzed using polymerase chain reaction to identify the nuc, tuf, tst, sea, pathogenic activity genes. After this, the bacteria were tested for ermA, ermB, ermC genes and for mecA, femA which are involved in resistance to macrolides, lincosamides, streptogramin B and to beta-lactams, respectively. The presence or the absence of these genes confirms that tested strains are resistant to specific antibiotic or not. Bacteria pathogenic activity was emphasized by genes as follows: sea (enterotoxin which was found at all isolates, tst (toxic shock toxin gene was not detected in any of isolates and tuf gene (elongation factor was obtained with one pair of primers. Resistance to beta-lactams was evidenced by the presence of mecA in all isolates and femA in some strains. Each of ermC, ermA and ermB, macrolides-lincosamides-streptogramin B resistance genes, were detected.

  6. Inducible and Acquired Clarithromycin Resistance in the Mycobacterium abscessus Complex.

    Directory of Open Access Journals (Sweden)

    Marc Rubio

    Full Text Available Clarithromycin was considered the cornerstone for the treatment of Mycobacterium abscessus complex infections. Genetic resistance mechanisms have been described and many experts propose amikacin as an alternative. Nevertheless, clarithromycin has several advantages; therefore, it is necessary to identify the non-functional erm(41 allele to determine the most suitable treatment. The aims of this study were to characterize the molecular mechanisms of clarithromycin resistance in a collection of Mycobacterium abscessus complex isolates and to verify the relationship between these mechanisms and the antibiogram.Clinical isolates of M. abscessus complex (n = 22 from 16 patients were identified using four housekeeping genes (rpoB, secA1, sodA and hsp65, and their genetic resistance was characterized by studying erm(41 and rrl genes. Nine strains were recovered from the clinical isolates and subjected to E-test and microdilution clarithromycin susceptibility tests, with readings at 3, 7 and 14 days.We classified 11/16 (68.8% M. abscessus subsp. abscessus, 4/16 (25.0% M. abscessus subsp. bolletii, and 1/16 (6.3% M. abscessus subsp. massiliense. T28 erm(41 allele was observed in 8 Mycobacterium abscessus subps. abscessus and 3 Mycobacterium abscessus subsp. bolletii. One strain of M. abscessus subsp. bolletii had an erm(41 gene truncated and was susceptible to clarithromycin. No mutations were observed in rrl gene first isolates. In three patients, follow-up of initial rrl wild-type strains showed acquired resistance.Most clinical isolates of M. abscessus complex had inducible resistance to clarithromycin and total absence of constitutive resistance. Our findings showed that the acquisition of resistance mutations in rrl gene was associated with functional and non-functional erm(41 gene. Caution is needed when using erm(41 sequencing alone to identify M. abscessus subspecies. This study reports an acquired mutation at position 2057 of rrl gene

  7. Establishment and characterization of novel epithelial-like cell lines derived from human periodontal ligament tissue in vitro.

    Science.gov (United States)

    Tansriratanawong, Kallapat; Ishikawa, Hiroshi; Toyomura, Junko; Sato, Soh

    2017-10-01

    In this study, novel human-derived epithelial-like cells (hEPLCs) lines were established from periodontal ligament (PDL) tissues, which were composed of a variety of cell types and exhibited complex cellular activities. To elucidate the putative features distinguishing these from epithelial rest of Malassez (ERM), we characterized hEPLCs based on cell lineage markers and tight junction protein expression. The aim of this study was, therefore, to establish and characterize hEPLCs lines from PDL tissues. The hEPLCs were isolated from PDL of third molar teeth. Cellular morphology and cell organelles were observed thoroughly. The characteristics of epithelial-endothelial-mesenchymal-like cells were compared in several markers by gene expression and immunofluorescence, to ERM and human umbilical-vein endothelial cells (HUVECs). The resistance between cellular junctions was assessed by transepithelial electron resistance, and inflammatory cytokines were detected by ELISA after infecting hEPLCs with periodontopathic bacteria. The hEPLCs developed into small epithelial-like cells in pavement appearance similar to ERM. However, gene expression patterns and immunofluorescence results were different from ERM and HUVECs, especially in tight junction markers (Claudin, ZO-1, and Occludins), and endothelial markers (vWF, CD34). The transepithelial electron resistance indicated higher resistance in hEPLCs, as compared to ERM. Periodontopathic bacteria were phagocytosed with upregulation of inflammatory cytokine secretion within 24 h. In conclusion, hEPLCs that were derived using the single cell isolation method formed tight multilayers colonies, as well as strongly expressed tight junction markers in gene expression and immunofluorescence. Novel hEPLCs lines exhibited differently from ERM, which might provide some specific functions such as metabolic exchange and defense mechanism against bacterial invasion in periodontal tissue.

  8. Evolution and molecular characterization of macrolide-resistant Streptococcus pneumoniae in Canada between 1998 and 2008.

    Science.gov (United States)

    Wierzbowski, Aleksandra K; Karlowsky, James A; Adam, Heather J; Nichol, Kim A; Hoban, Daryl J; Zhanel, George G

    2014-01-01

    Studies were performed to assess resistance mechanisms, multidrug resistance (MDR), genetic relatedness, serotype distribution, heptavalent pneumococcal conjugate vaccine (PCV7) coverage and pili virulence factors among macrolide-resistant Streptococcus pneumoniae (MRSP) isolated from respiratory samples submitted to hospital laboratories across Canada from 1998 to 2008. Isolates of MRSP (n = 1518) collected by the national surveillance studies CROSS (Canadian Respiratory Organism Susceptibility Study; 1998-2006) and CANWARD (Canadian Ward Surveillance Study; 2007-08) were tested using the CLSI broth microdilution method to establish antimicrobial susceptibilities. PCR was used to detect macrolide resistance genes [mef(A) and erm(B)] and pili virulence factors (type 1 pili and type 2 pili), the Quellung reaction was used to identify serotypes and PFGE was used to determine genetic relatedness. The prevalence of MRSP increased from 8% in 1998 to 22% in 2008 (P = 0.0001). MRSP were 51% mef(A) positive, 36% erm(B) positive, 8% dual mef(A) and erm(B) positive and 5% mef(A) and erm(B) negative. Dual mef(A)- and erm(B)-positive isolates increased in prevalence from 3% in 1998 to 19% in 2008 (P = 0.001). The prevalence of PCV7 serotypes (4, 6B, 9V, 14, 18C, 19F and 23F) decreased from 67% in 1998 to 31% in 2008 (P = 0.0072). The prevalence of serotype 19A, a non-PCV7 serotype, increased by 15% from 1998 to 2008; isolates of serotype 19A were MDR, dual mef(A) and erm(B) positive, genetically related by PFGE and associated with the presence of pili virulence factors. From 1998 to 2008, respiratory isolates of MRSP in Canada increased significantly due primarily to the emergence and spread of serotypes 6A, 19A and other non-PCV7 serotypes.

  9. Change in refraction after lens-sparing vitrectomy for rhegmatogenous retinal detachment and epiretinal membrane.

    Science.gov (United States)

    Iwase, Takeshi; Yamamoto, Kentaro; Yanagida, Kosei; Kobayashi, Misato; Ra, Eimei; Murotani, Kenta; Terasaki, Hiroko

    2016-08-01

    The aim of this study was to compare changes in refraction following lens-sparing vitrectomy between patients with rhegmatogenous retinal detachment (RRD) and epiretinal membrane (ERM) and to investigate factors associated with the change in refraction.We reviewed medical records of 49 eyes of 49 patients with RRD (53.6 ± 7.8 years, mean ± standard deviation) and 24 eyes of 24 patients with ERM (50.9 ± 15.7 years) who underwent lens-sparing vitrectomy. Spherical equivalent refractive power was evaluated before and up to 18 months after surgery. The relationship between the change in refraction and several parameters was evaluated.A significant progressive myopic shift in refractive power was observed after vitrectomy in operated RRD and ERM eyes (P refraction values observed at ≥3 and ≥12 months following vitrectomy were significantly different as compared with those observed at baseline in the RRD group (P refraction between the RRD and ERM groups was significant (P = 0.030). The multiple linear regression analysis showed that only age was significantly correlated with the change in refraction in RRD (P = 0.018) and ERM (P refraction was significantly and positively correlated with age in RRD (r = -0.461, P = 0.001) and ERM (r = -0.687, P refraction owing to nuclear sclerosis was observed. Core vitrectomy itself would cause a myopic shift of refraction. The only risk factor associated with cataract progression following lens-sparing vitrectomy is age for both types of patients.

  10. Expansión rápida maxilar. Informe de un caso.

    OpenAIRE

    Germán Puerta

    2009-01-01

    La expansión maxilar rápida (ERM)se ha usado desde mediados del siglo XIX como alternativa para la corrección de mordidas cruzadas posteriores. Se realizó una importante búsqueda bibliográfica acerca de la ERM, sus logros y efectos sobre el complejo cráneo-facial. Se presenta un caso clínico exitoso donde se pudo comprobar cómo la mayoría de los cambios citados por diferentes autores se manifestaron en la paciente.

  11. Lattice point sets for deterministic learning and approximate optimization problems.

    Science.gov (United States)

    Cervellera, Cristiano

    2010-04-01

    In this brief, the use of lattice point sets (LPSs) is investigated in the context of general learning problems (including function estimation and dynamic optimization), in the case where the classic empirical risk minimization (ERM) principle is considered and there is freedom to choose the sampling points of the input space. Here it is proved that convergence of the ERM principle is guaranteed when LPSs are employed as training sets for the learning procedure, yielding up to a superlinear convergence rate under some regularity hypotheses on the involved functions. Preliminary simulation results are also provided.

  12. Professional Monograph Review

    Directory of Open Access Journals (Sweden)

    Mildeová Stanislava

    2008-10-01

    Full Text Available Title of the monograph: Systems Approach to Knowledge Modelling. Authors: dr. Ludmila Dömeová; dr. Milan Houška; dr. Martina Houšková Beránková. Cover designer: Olga Čermáková. Interior designer: Roman Kvasnička. Publisher: Graphical Studio Olga Čermáková, Czech Republic. Place: Hradec Králové, Czech Republic. Year of publication: 2008. Number of pages: 282. Recommended price of the book: 39.90 EUR. First edition. Reviewer : dr. Stanislava Mildeová; Department of Systems Analysis, Faculty of Informatics and Statistics, University of Economics, Prague.

  13. Unistused muudavad maailma / Enn Tammearu

    Index Scriptorium Estoniae

    Tammearu, Enn

    2000-01-01

    Rocca al Mare koolimaja ehitamisest, AS KGM Ehitus meeskonnast ja juhatuse esimehest Tiit Nurklikust. Projekti autor: AB Urbel & Peil. Arhitektid Emil Urbel, Indrek Erm, sisearhitekt Taso Mähar, projektijuht Ivar Aus. Konstruktiivne osa: E-Inseneribüroo. Kooli direktori Rein Rebase, I. Ausi, I. Ermi ja T. Nurkliku kommentaarid. Nr. 11, lk. 4-6: Rocca al Mare koolile 2000. a. parima betoonehitise tiitel. Akustik Linda Madalik. Monoliitbetoonitööd: AS Roxor Ehitus. Arvamust avaldavad: T. Nurklik, I. Erm, OÜ Roxoe Ehituse tehniline juht Kalev Ramjalg, žürii esimees Aadu Kana

  14. Serotypes and antibiotic resistance in Group B streptococcus isolated from patients at the Maternity Hospital, Kuwait.

    Science.gov (United States)

    Boswihi, Samar S; Udo, Edet E; Al-Sweih, Noura

    2012-01-01

    A total of 143 group B streptococcus (GBS) isolates collected from mothers at the Maternity Hospital in Kuwait were investigated for their serotypes and antibiotic resistance, and screened by PCR for the carriage of genes for resistance to tetracycline (tetk, tetM, tetL, tetO), erythromycin (ermA, ermB, ermC, ermTR, ermM, mefA, mefE, msrA) and aminoglycosides (aph3, ant4, ant6). All isolates were serotyped using a latex agglutination test. Most of the isolates belonged to serotypes V (38.5 %), III (20.9 %), Ia (7.7 %) and II (11.2 %). Sixteen isolates (11.2 %) were nontypable. All isolates were susceptible to penicillin, ampicillin and cefotaxime (MICs 0.016-0.094 µg ml(-1)) but were resistant to trimethoprim (92.3 %), tetracycline (89.5 %), minocycline (89.5 %), high-level kanamycin (76.9 %), chloramphenicol (30.0 %), erythromycin (12.6 %), clindamycin (7.0 %), high-level streptomycin (3.5 %) and ciprofloxacin (0.7 %). The tetracycline-resistant isolates contained tetM (94.5 %), tetO (3.9 %), tetL (1.6 %) and tetK (0.8 %). The erythromycin-resistant isolates contained ermB (61.1 %), ermTR (38.9 %), ermA (5.5 %), mefA (5.5 %) and mefE (11 %). All high-level kanamycin-resistant isolates contained aph3. One of the high-level streptomycin-resistant isolates contained ant6. Partial DNA sequencing of aph3 revealed sequences with 99 % similarity to aph3 found in Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis, suggesting that the GBS isolates could have acquired aph3 from other Gram-positive cocci. The high proportion of isolates with resistance to tetracycline, high-level kanamycin and trimethoprim, and the increase in the prevalence of erythromycin resistance, represents an emerging public health concern that needs further surveillance.

  15. Strategien für die Melissezüchtung (Melissa officinalis

    Directory of Open Access Journals (Sweden)

    Kittler, Johannes

    2014-09-01

    Full Text Available Es wurden 120 Melisseherkünfte in Feldversuchen evaluiert. In diesem Material wurde die Variabilität für Winterhärte, Gehalt und Zsammensetzung des ätherischen Öls, Rosmarinsäuregehalt und eingeschränkt Blattertrag beschrieben. Die beschriebene Variabilität ermöglichte die Entwicklung homozygoter Linien mit hoher Eigenleistung für die genannten Zuchtziele bis zur I4-Inzuchtgeneration. Die Kreuzung definierter Linien ermöglichte die Schaffung eines neuen Genpools als Ausgansmaterial für die Entwicklung weiterer aussichtsreicher Linien.

  16. Mycorrhizas and dark septate root endophytes in polar regions

    OpenAIRE

    Newsham, K.K.; Upson, R.; Read, D J

    2009-01-01

    We review the distributions and functions of mycorrhizas and dark septate root endophytes in polar regions. Arbuscular mycorrhizas (AM) are present in the Arctic and Antarctic to 82 ºN and 63 ºS, respectively, with fine endophyte being the dominant form of AM in roots at higher latitudes. Ecto- (ECM) and ericoid (ERM) mycorrhizas both occur in the Arctic to 79 ºN, owing to the presence of species of Salix, Dryas, Vaccinium and Cassiope to this latitude. ECM and ERM are not present in Antarcti...

  17. Implementation of an enterprise risk-management program in a community teaching hospital.

    Science.gov (United States)

    Behamdouni, Genefer; Millar, Kathy

    2010-01-01

    As the complexity of healthcare and expectations of comprehensive and transparent public accountability heighten, so too must a hospital's approach to assessing and managing risk. Over a period of two years, the area of patient safety and risk at our hospital has moved from a traditional focus on clinical risk management to an enterprise-wide risk management approach. One of the first community hospitals to embrace enterprise risk management (ERM), St. Joseph's Health Centre, in Toronto, Ontario, has seen early benefits in this transformational journey. This article discusses our approach to the development of an ERM program, tools used and lessons learned.

  18. Identification of antimicrobial resistance genes in multidrug-resistant clinical Bacteroides fragilis isolates by whole genome shotgun sequencing

    DEFF Research Database (Denmark)

    Sydenham, Thomas Vognbjerg; Sóki, József; Hasman, Henrik

    2015-01-01

    ://cge.cbs.dtu.dk/services/ResFinder/) and a custom BLAST database. Combinations of cfxA, cepA, cfiA, nimA, nimD, nimE, nimJ, tetQ, ermB, ermF, bexB, linAn2 and mefEn2 genes were identified in the six isolates. blaOXA-347, an open reading frame predicted to be a β-lactamase (Cheng et al., 2012), was identified in one strain. Full length IS elements...

  19. Learning Faster by Discovering and Exploiting Object Similarities

    Directory of Open Access Journals (Sweden)

    Tadej Janež

    2013-03-01

    Full Text Available In this paper we explore the question: “Is it possible to speed up the learning process of an autonomous agent by performing experiments in a more complex environment (i.e., an environment with a greater number of different objects?” To this end, we use a simple robotic domain, where the robot has to learn a qualitative model predicting the change in the robot's distance to an object. To quantify the environment's complexity, we defined cardinal complexity as the number of objects in the robot's world, and behavioural complexity as the number of objects' distinct behaviours. We propose Error reduction merging (ERM, a new learning method that automatically discovers similarities in the structure of the agent's environment. ERM identifies different types of objects solely from the data measured and merges the observations of objects that behave in the same or similar way in order to speed up the agent's learning. We performed a series of experiments in worlds of increasing complexity. The results in our simple domain indicate that ERM was capable of discovering structural similarities in the data which indeed made the learning faster, clearly superior to conventional learning. This observed trend occurred with various machine learning algorithms used inside the ERM method.

  20. Reaction and Transport Processes Controlling In Situ Chemical Oxidation of DNAPLs

    Science.gov (United States)

    2006-11-01

    HydroGeoLogic, Inc., Phoenix, Arizona Dr. Dick Brown – ERM, Ewing, New Jersey Mr. Michael C. Marley - XDD, LLC, Stratham, New Hampshire Mr. Bob Norris...Remediation Journal, 14(2):7-31. Droste, E.X., M.C. Marley , J.M. Parikh, and A.M. Lee (2002). Observed Enhanced Reductive Dechlorination after In Situ

  1. The effect of z-binding yarns on the electrical properties of 3D woven composites

    KAUST Repository

    Saleh, Mohamed Nasr

    2017-09-28

    Electrical resistance monitoring (ERM) has been used to study the effect of the z-binding yarns on the initial electrical resistance (ER) and its change of three architectures of 3D woven carbon fibre composites namely (orthogonal “ORT”, layer-to-layer “LTL” and angle interlock “AI”) when tested in tension. Specimens are loaded in on-axis “warp” and off-axis “45°” directions. In-situ ERM is achieved using the four-probe technique. Monotonic and cyclic “load/unload” tests are performed to investigate the effect of piezo-resistivity and residual plasticity on resistance variation. The resistance increase for the off-axis loaded specimens (∼90%) is found to be higher than that of their on-axis counterparts (∼20%). In the case of cyclic testing, the resistance increase upon unloading is irreversible which suggests permanent damage presence not piezo-resistive effect. At the moment, it is difficult to obtain a direct correlation between resistance variation and damage in 3D woven composites due to the complexity of the conduction path along the three orthogonal directions, however this study demonstrates the potential of using ERM for damage detection in 3D woven carbon fibre-based composites and highlights the challenges that need to be overcome to establish ERM as a Structural Health Monitoring (SHM) technique for such material systems.

  2. LKB1 Is Required for the Development and Maintenance of Stereocilia in Inner Ear Hair Cells in Mice.

    Directory of Open Access Journals (Sweden)

    Yuqin Men

    Full Text Available The LKB1 gene, which encodes a serine/threonine kinase, was discovered to play crucial roles in cell differentiation, proliferation, and the establishment of cell polarity. In our study, LKB1 conditional knockout mice (Atoh1-LKB1-/- mice were generated to investigate LKB1 function in the inner ear. Tests of auditory brainstem response and distortion product otoacoustic emissions revealed significant decreases in the hearing sensitivities of the Atoh1-LKB1-/- mice. In Atoh1-LKB1-/- mice, malformations of hair cell stereocilliary bundles were present as early as postnatal day 1 (P1, a time long before the maturation of the hair cell bundles. In addition, we also observed outer hair cell (OHC loss starting at P14. The impaired stereocilliary bundles occurred long before the presence of hair cell loss. Stereociliary cytoskeletal structure depends on the core actin-based cytoskeleton and several actin-binding proteins. By Western blot, we examined actin-binding proteins, specifically ERM (ezrin/radixin/moesin proteins involved in the regulation of the actin cytoskeleton of hair cell stereocilia. Our results revealed that the phosphorylation of ERM proteins (pERM was significantly decreased in mutant mice. Thus, we propose that the decreased pERM may be a key factor for the impaired stereocillia function, and the damaged stereocillia may induce hair cell loss and hearing impairments. Taken together, our data indicates that LKB1 is required for the development and maintenance of stereocilia in the inner ear.

  3. A composite transposon associated with erythromycin and clindamycin resistance in group B Streptococcus.

    Science.gov (United States)

    Puopolo, Karen M; Klinzing, David C; Lin, Michelle P; Yesucevitz, Derek L; Cieslewicz, Michael J

    2007-07-01

    Group B Streptococcus (GBS) resistant to erythromycin and clindamycin has been isolated with increasing frequency since the mid-1990s. This work studied GBS isolates from three US cities to determine the genetic basis of the macrolide resistance phenotype. ermB genes were amplified from five isolates collected in Boston, Pittsburgh and Seattle from infant and adult sources. Gene-walking methods were used to determine the chromosomal location of ermB and to identify associated genes. Southern mapping and random amplified polymorphic DNA (RAPD) analyses were used to distinguish the isolates. The ermB gene was present on the chromosome within a composite Tn917/Tn916-like transposon similar to one identified in Streptococcus pneumoniae. Four strains from Boston and Pittsburgh were serotype V and identical by Southern hybridization and RAPD analysis. The Seattle isolate was serotype Ib, with different patterns on RAPD analysis and Southern mapping. The composite transposon was integrated at an identical chromosomal site in all five isolates. The presence of this composite transposon in both GBS and pneumococci suggests that ermB-mediated macrolide resistance in streptococci may be due to the horizontal transfer of a mobile transposable element, and raises concern for further dissemination of high-grade erythromycin and clindamycin resistance among streptococcal species.

  4. David Lynchi lamella / Slavoj Žižek ; tõlkinud Hasso Krull

    Index Scriptorium Estoniae

    Žižek, Slavoj, 1949-

    1996-01-01

    Režissöör ja tema filmide psühhoanalüütiline analüüs, tõlge kogumikust "Reading Seminar XI. Lacan's Four Fundamental Concepts of Psychoanalysis", 1995. Lisad : tõlkija seletab sõna "lamella" tähenduse, Aare Ermeli koostatud režissööri filmograafia (mittetäielik)

  5. SACE_3986, a TetR family transcriptional regulator, negatively controls erythromycin biosynthesis in Saccharopolyspora erythraea.

    Science.gov (United States)

    Wu, Panpan; Pan, Hui; Zhang, Congming; Wu, Hang; Yuan, Li; Huang, Xunduan; Zhou, Ying; Ye, Bang-ce; Weaver, David T; Zhang, Lixin; Zhang, Buchang

    2014-07-01

    Erythromycin, a medically important antibiotic, is produced by Saccharopolyspora erythraea. Unusually, the erythromycin biosynthetic gene cluster lacks a regulatory gene, and the regulation of its biosynthesis remains largely unknown. In this study, through gene deletion, complementation and overexpression experiments, we identified a novel TetR family transcriptional regulator SACE_3986 negatively regulating erythromycin biosynthesis in S. erythraea A226. When SACE_3986 was further inactivated in an industrial strain WB, erythromycin A yield of the mutant was increased by 54.2 % in average compared with that of its parent strain, displaying the universality of SACE_3986 as a repressor for erythromycin production in S. erythraea. qRT-PCR analysis indicated that SACE_3986 repressed the transcription of its adjacent gene SACE_3985 (which encodes a short-chain dehydrogenase/reductase), erythromycin biosynthetic gene eryAI and the resistance gene ermE. As determined by EMSA analysis, purified SACE_3986 protein specifically bound to the intergenic region between SACE_3985 and SACE_3986, whereas it did not bind to the promoter regions of eryAI and ermE. Furthermore, overexpression of SACE_3985 in A226 led to enhanced erythromycin A yield by at least 32.6 %. These findings indicate that SACE_3986 is a negative regulator of erythromycin biosynthesis, and the adjacent gene SACE_3985 is one of its target genes. The present study provides a basis to increase erythromycin production by engineering of SACE_3986 and SACE_3985 in S. erythraea.

  6. Macrolide resistance conferred by rRNA mutations in field isolates of Mannheimia haemolytica and Pasteurella multocida

    DEFF Research Database (Denmark)

    Olsen, Anders S; Warrass, Ralf; Douthwaite, Stephen Roger

    2014-01-01

    . haemolytica identified as being highly resistant (MICs >64 mg/L) to the macrolides erythromycin, gamithromycin, tilmicosin, tildipirosin and tulathromycin were screened by multiplex PCR for the previously identified resistance genes erm(42), msr(E) and mph(E). Strains lacking these determinants were analysed...

  7. Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in studies of phage biology

    DEFF Research Database (Denmark)

    Koch, Birgit; Christiansen, Bettina; Evison, Tim

    1997-01-01

    A method for the construction and isolation of specifically designed mutations of the temperate lactococcal phage TP901-1 has been developed. Two different erm-labeled mutants were isolated. One was shown to be defective in lysogenization and excision. The other, showing normal lysogenization, wa...

  8. Disease: H00037 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ith the skeletal muscle lineage, although those tumors can be present in organs histologically lacking skele...geneous into two major histologic subtypes, embryonal (ERMS) and alveolar (ARMS). Alveolar rhabdomyosarcoma ...tal muscle, like prostate, urinary bladder or gallbladder. RMS comprises a hetero

  9. Checklist Manifesto for Electronic Resources: Getting Ready for the Fiscal Year and Beyond

    Science.gov (United States)

    England, Lenore; Fu, Li; Miller, Stephen

    2011-01-01

    Organization of electronic resources workflow is critical in the increasingly complicated and complex world of library management. A simple organizational tool that can be readily applied to electronic resources management (ERM) is the use of checklists. Based on the principles discussed in The Checklist Manifesto: How to Get Things Right, the…

  10. High frequency of macrolide resistance mechanisms in clinical isolates of Corynebacterium species.

    Science.gov (United States)

    Ortiz-Pérez, Alberto; Martín-de-Hijas, Nieves Z; Esteban, Jaime; Fernández-Natal, María Isabel; García-Cía, José Ignacio; Fernández-Roblas, Ricardo

    2010-12-01

    The genus Corynebacterium includes a high number of species that are usually isolated from human skin as saprophytes. However, these microorganisms have also been reported as infectious agents in a broad group of patients and have showed broad-spectrum resistance. We studied the susceptibility profiles against macrolides, clindamycin, and streptogramins of 254 clinical strains belonging to the species Corynebacterium urealyticum (120), Corynebacterium amycolatum (66), Corynebacterium jeikeium (17), Corynebacterium striatum (20), Corynebacterium coyleae (12), Corynebacterium aurimucosum (11), and Corynebacterium afermentans subsp. afermentans (8). The MLS(B) phenotype was detected in 186 strains and was associated with the presence of methylase enzymes codified by the erm(X) gene in 171 strains. The erm(B) gene was only detected in two C. urealyticum strains. Fourteen strains showed macrolide resistance, but they did not carry erm genes. mef genes were not detected despite eight C. amycolatum strains showed the M phenotype. Also, the presence of hydrolytic enzymes codified by ere(B) was evaluated, but all results were negative. Resistance to macrolide in Corynebacterium sp. is mainly due to the presence of erm(X) methylase, although other resistance mechanisms could be involved.

  11. Isotopic Constraints on the Sources and Associations of Organic Compounds in Marine Sediments

    Science.gov (United States)

    2006-02-01

    carbon. This fossil carbon is derived from kerogen from bedrock in the case of ERM sediments (Blair et al., 2004); pre-aged soil material and bitumen ...and agitating for lhr. Following centrifugation, the supematant was removed and this was repeated twice more. Silicate minerals were then removed by

  12. Comparative evaluation of administration methods for a vaccine protecting rainbow trout against Yersinia ruckeri O1 biotype 2 infections

    DEFF Research Database (Denmark)

    Chettri, Jiwan Kumar; Deshmukh, Sidhartha; Holten-Andersen, Lars

    2013-01-01

    Numerous outbreaks of enteric red mouth disease (ERM) caused by Yersinia ruckeri O1 biotype 2 in rainbow trout farms are currently being recorded despite established vaccination procedures against this disease. This could indicate that the currently used application of single immersion vaccination...

  13. Vestlusring : Tööturul valitseb segadus / interv. Tõnis Tõnisson

    Index Scriptorium Estoniae

    2005-01-01

    Tööst ja töötusest Raplamaal vestlevad Raplamaa Arendus- ja Ettevõtluskeskuse juhataja Janek Kadarik, projektijuht Rando Lai, Raplamaa Noorte Teabekeskuse juht Marje Reimund, Raplamaa Tööhõiveameti direktor Vahur Soikmets, Rapla Ettevõtjate Ühenduse juhatuse esimees Raivo Erm ja Nädalise reklaamisosakonna juhataja Kaja Ohaka

  14. Linear Logistic Test Modeling with R

    Science.gov (United States)

    Baghaei, Purya; Kubinger, Klaus D.

    2015-01-01

    The present paper gives a general introduction to the linear logistic test model (Fischer, 1973), an extension of the Rasch model with linear constraints on item parameters, along with eRm (an R package to estimate different types of Rasch models; Mair, Hatzinger, & Mair, 2014) functions to estimate the model and interpret its parameters. The…

  15. E-Resource Statistics: What to Do when You Have No Money

    Science.gov (United States)

    Walker, Mary

    2009-01-01

    Libraries are moving toward electronic resource management systems (ERMSs) to track their usage statistics, but these can be expensive to purchase and maintain. For some libraries, an ERMS can be cost-prohibitive, but they still need to justify the renewal of databases and e-journals to their budget officers or determine which e-resources should…

  16. Monetary and Exchange Rate Policy in Slovenia

    OpenAIRE

    Gonzalo Caprirolo; Vladimir Lavrac

    2003-01-01

    The paper is divided in three sections presenting some stylized facts concerning monetary and exchange rate policy framework in Slovenia. Three periods are covered: Money-based Stabilization Policy (1991-1995), Price and Real Exchange Rate Stability Dual Targeting Policy (1996-2001), and Exchange Rate Based Stabilization Policy and Accession to ERM2 (2001-).

  17. Real-Time Effects of Central Bank Interventions in the Euro Market

    DEFF Research Database (Denmark)

    Fatum, Rasmus; Pedersen, Jesper

    This paper investigates the real-time effects of foreign exchange intervention using official intraday intervention data provided by the Danish central bank. Denmark is currently pursuing an active intervention policy under the provisions of the Exchange Rate Mechanism (ERM II) and intervenes...

  18. Sellest, mis teiste eest varjatud / Rainer Sarnet

    Index Scriptorium Estoniae

    Sarnet, Rainer, 1969-

    2000-01-01

    USA mängufilmid "Tabamatu ilu" (American Beauty") : režissöör Sam Mendes : 1999 ja "Õnn" ("Happiness") : režissöör Todd Solondz : 1998. Lisa : Aare Ermeli koostatud režissööride lühielulood

  19. Inducible clindamycin resistance and nasal carriage rates of ...

    African Journals Online (AJOL)

    Clindamycin, the macrolide-lincosamide-streptogramin. B (MLSB) antimicrobial group is an alternative treat ... among members of the MLSB10. The most common mechanism of macrolide resistance is mediated by erm ..... trends of pediatric community-acquired methicillin-re- sistant Staphylococcus aureus in Dallas, Texas.

  20. De overdracht van risico’s in de zorg : Risicobeheersing in een instelling voor langdurige ouderenzorg

    NARCIS (Netherlands)

    van der Steen, Martijn

    Dit artikel analyseert een kleine instelling voor ouderenzorg die blootstaat aan verscheidene risico’s als gevolg van de wettelijke veranderingen in de zorgsector. Hoewel kleinere instellingen voor langdurige ouderenzorg niet altijd gestructureerde methodieken als COSO ERM of ISO 31000 voor de

  1. Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri i.p. vaccination

    DEFF Research Database (Denmark)

    Raida, Martin Kristian; Buchmann, Kurt

    2007-01-01

    The immune response in rainbow trout against a bacterin of Yersinia ruckeri, a bacterial pathogen causing enteric red mouth disease (ERM), was investigated at 5, 15 and 25° C. Rainbow trout were immunized by i.p. injection of a Y. ruckeri (serotype O1) water based bacterin and compared to control...

  2. 77 FR 37038 - Clean Air Act Operating Permit Program; Petition for Objection to State Operating Permit for CF&I...

    Science.gov (United States)

    2012-06-20

    ... Permit Program; Petition for Objection to State Operating Permit for CF&I Steel, L.P. dba EVRAZ Rocky... December 28, 2010 Permit (Permit) issued to CF&I Steel, L.P. dba EVRAZ Rocky Mountain Steel (ERMS or EVRAZ... should make an appointment at least 24 hours in advance. Additionally, the final Order for CF&I Steel, L...

  3. Teaching Electronic Records Management in the Archival Curriculum

    Science.gov (United States)

    Zhang, Jane

    2016-01-01

    Electronic records management has been incorporated into the archival curriculum in North America since the 1990s. This study reported in this paper provides a systematic analysis of the content of electronic records management (ERM) courses currently taught in archival education programs. Through the analysis of course combinations and their…

  4. Molecular subtyping and erythromycin resistance of Campylobacter in China.

    Science.gov (United States)

    Zhang, A; Song, L; Liang, H; Gu, Y; Zhang, C; Liu, X; Zhang, J; Zhang, M

    2016-07-01

    To investigate the erythromycin resistance patterns and mechanism for Campylobacter isolates in China. The minimum inhibitory concentrations of erythromycin on 858 Chinese Campylobacter isolates were analysed. PCR and DNA sequencing were used to identify mutations in the 23S rRNA and the presence of the ermB gene in the 158 erythromycin resistance isolates (18·4%). About 83% (131/158) had A2075G mutation in their 23S rRNA; no A2074C/G mutants were found. The ermB gene was identified in 30 Campylobacter coli isolates (19%). Four types of multidrug-resistant gene islands (MDRGIs) were found. Fifty-three types were identified by multilocus sequence typing among the resistant isolates. All isolates of STs 6322 and 1145 had the ermB gene. The erythromycin resistance rate of Camp. coli (58·56%) was much higher than Campylobacter jejuni (0·67%). The insertion sites between cadF and CCO1582 and between nfsB and cinA on the chromosome might be hot spots for MDRGI transformation. Point mutation in domain V of the 23S rRNA and the ermB gene accounted for 100% of the erythromycin resistance of Campylobacter in China. Journal of Applied Microbiology © 2016 The Society for Applied Microbiology.

  5. Tumor-suppression functions of merlin are independent of its role as an organizer of the actin cytoskeleton in Schwann cells.

    Science.gov (United States)

    Lallemand, Dominique; Saint-Amaux, Aurelie Lampin; Giovannini, Marco

    2009-11-15

    Merlin is the product of the Nf2 tumor-suppressor gene, and inactivation of Nf2 leads to the development of neural tumors such as schwannomas and meningiomas in humans and mice. Merlin is a member of the ERM (ezrin, radixin and moesin) family of proteins that function as organizers of the actin cytoskeleton. Merlin structure is thought to be similar to that of the ERM proteins, and is held in a closed clamp conformation via intramolecular interactions of its N-terminal FERM (four-point-one, ERM) domain with an alpha-helical C-terminal domain. Like ERMs, merlin can remodel actin-rich cortical structures, yet merlin uniquely inhibits the proliferation of many different cell types. Here, we report that the F2 subdomain of the FERM domain and a domain close to the C-terminus that is defined by residues 532-579 are essential for merlin-mediated inhibition of primary Schwann cell proliferation. Furthermore, we demonstrate that the F1 subdomain of the merlin FERM domain is required for actin colocalization, proper regulation of merlin C-terminal phosphorylation and for remodeling the cytoskeleton, yet is not required for the inhibition of Schwann cell proliferation. Thus, tumor suppression by merlin is independent of its role as an organizer of the actin cytoskeleton in Schwann cells.

  6. 76 FR 45907 - Implementation of the Amendments to the International Convention on Standards of Training...

    Science.gov (United States)

    2011-08-01

    .... Civil Justice Reform I. Protection of Children J. Indian Tribal Governments K. Energy Effects L... Exclusive Economic Zone ERM Engine Room Resource Management FCC Federal Communications Commission F.H. Food... proposing to use the term proficiency in survival craft and rescue boats other than lifeboats and fast...

  7. Trade off study on different envelope detectors for B-mode imaging

    DEFF Research Database (Denmark)

    Schlaikjer, Malene; Bagge, J. P.; Jensen, Jørgen Arendt

    2003-01-01

    to REC, while maintaining a performance close to IDE. Two low-cost detectors have been implemented and evaluated on in-vivo data. The first approach is an expansion of the rectifier with a median filter (ERM). The second detector (TAS) approximates the HT by a time delay and the magnitude by a weighted...

  8. Euroopa arhitektuuriauhind Rafael Moneole / Ingrid Lillemägi

    Index Scriptorium Estoniae

    Lillemägi, Ingrid

    2001-01-01

    2001. a. Mies van der Rohe preemia võitjad: peapreemia - hispaania arhitekt Rafael Moneo, noore arhitekti preemia - saksa arhitekt Florian Nagler. Eestist esitati preemia saamiseks De la Gardie' kaubamaja (Andres Alver, Tiit Trummal, Tarmo Laht), Rocca al Mare koolimaja (Emil Urbel, Indrek Erm, Ülo Peil, Taso Mähar) ja elamud Suurupis (Urmas Muru, Peeter Pere). 1 ill

  9. Quantification of retinal tangential movement in epiretinal membranes

    DEFF Research Database (Denmark)

    Kofod, Mads; la Cour, Morten

    2012-01-01

    To describe a technique of quantifying retinal vessel movement in eyes with epiretinal membrane (ERM) and correlate the retinal vessel movement with changes in best-corrected visual acuity (BCVA), central macular thickness (CMT), and patients' subjective reports about experienced symptoms (sympto...

  10. Antimicrobial Drug Use and Macrolide-Resistant Streptococcus pyogenes, Belgium

    OpenAIRE

    Van Heirstraeten, Liesbet; Coenen, Samuel; Lammens, Christine; Hens, Niel; Goossens, Herman; Malhotra-Kumar, Surbhi

    2012-01-01

    In Belgium, decreasing macrolide, lincosamide, streptogramins B, and tetracycline use during 1997–2007 correlated significantly with decreasing macrolide-resistant Streptococcus pyogenes during 1999–2009. Maintaining drug use below a critical threshold corresponded with low-level macrolide-resistant S. pyogenes and an increased number of erm(A)-harboring emm77 S. pyogenes with low fitness costs.

  11. Ülevaade Eesti varasest arvutikunstist / Tuuli Lepik

    Index Scriptorium Estoniae

    Lepik, Tuuli

    2004-01-01

    Eesti arvutikunsti arengust ja seda mõjutanud erinevatest nähtustest ning objektiivsetest teguritest. Ka Mall Nukke, Aavo Ermeli ja Tõnis Vindi stuudio esimestest katsetustest ning Rauno Remme, Ando Keskküla, Raivo Kelomehe ja teiste töödest

  12. Genetic and bibliographic information - GenLibi | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available n Data acquisition method Articles related to genes were obtained from the bibliography database (JDream II)...ism Organism of the gene Phenotype from JST bibliography Names of the diseases or...erms that are corresponding to the diseases or the phenotypes JST bibliography database IDs of the articles

  13. Simulated Annealing Approach Applied to the Energy Resource Management Considering Demand Response for Electric Vehicles

    DEFF Research Database (Denmark)

    Sousa, Tiago; Vale, Zita; Morais, Hugo

    2013-01-01

    The aggregation and management of Distributed Energy Resources (DERs) by an Virtual Power Players (VPP) is an important task in a smart grid context. The Energy Resource Management (ERM) of theses DERs can become a hard and complex optimization problem. The large integration of several DERs, incl...

  14. Un film très nonchalant / Kristiina Davidjants

    Index Scriptorium Estoniae

    Davidjants, Kristiina, 1974-

    2001-01-01

    Mängufilm "Hüvasti, kallis kodu" ("Adieu, plancher des vaches") : rezžissöör Otar Iosseliani : Prantsusmaa - Šveits - Itaalia 1999. Lisa : A. Ermeli koostatud režissööri lühielulugu ja filmograafia. Kokkuvõte inglise keeles lk. 96

  15. Getting insight into the prevalence of antibiotic resistance genes in specimens of marketed edible insects.

    Science.gov (United States)

    Milanović, Vesna; Osimani, Andrea; Pasquini, Marina; Aquilanti, Lucia; Garofalo, Cristiana; Taccari, Manuela; Cardinali, Federica; Riolo, Paola; Clementi, Francesca

    2016-06-16

    This study was aimed at investigating the occurrence of 11 transferable antibiotic resistance (AR) genes [erm(A), erm(B), erm(C), vanA, vanB, tet(M), tet(O), tet(S), tet(K), mecA, blaZ] in 11 species of marketed edible insects (small crickets powder, small crickets, locusts, mealworm larvae, giant waterbugs, black ants, winged termite alates, rhino beetles, mole crickets, silkworm pupae, and black scorpions) in order to provide a first baseline for risk assessment. Among the AR genes under study, tet(K) occurred with the highest frequency, followed by erm(B), tet(S) and blaZ. A high variability was seen among the samples, in terms of occurrence of different AR determinants. Cluster Analysis and Principal Coordinates Analysis allowed the 11 samples to be grouped in two main clusters, one including all but one samples produced in Thailand and the other including those produced in the Netherlands. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. Mariner-based transposon mutagenesis for Bacteroides species.

    Science.gov (United States)

    Ichimura, Minoru; Uchida, Keiko; Nakayama-Imaohji, Haruyuki; Hirakawa, Hideki; Tada, Tomoyo; Morita, Hidetoshi; Yasutomo, Koji; Okazaki, Katsuichiro; Kuwahara, Tomomi

    2014-06-01

    Bacteroides is one of the most predominant groups of human gut microbiota. Recent metagenomic analyses and studies on gnotobiotic mice demonstrated the tight association of Bacteroides with epithelial function, the gut immune system and systemic metabolism in the host. The mariner family transposon shows relatively low target site specificity and has hosts ranging from prokaryotes to eukaryotes. Thereby, random mutagenesis using the mariner family transposon is expected to identify key molecules for human-Bacteroides symbiosis. In this study, we constructed the plasmid pMI07 to deliver the gene cassette (ermF/ITR), which harbors the erythromycin resistant marker (ermF) and the inverted repeat sequences (ITRs) recognized by Himar1 transposase, to Bacteroides via electrotransformation. pMI07 successfully delivered ermF/ITR to the Bacteroides genomes and generated thousands of insertion mutants/μg of pMI07 in B. thetaiotaomicron, B. fragilis, B. ovatus, and also, although to a lesser extent, B. vulgatus. Analyses of the ermF/ITR insertion sites in B. thetaiotaomicron and B. vulgatus revealed that the cassette targeted the dinucleotide TA and integrated into the genomes in an unbiased manner. The data reported here will provide useful information for transposon mutagenesis in Bacteroides species, which will enable identification of the genes responsible for their unique phenotypes. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Pilvepiiri töötarud / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2009-01-01

    Tallinna kõrghoonetest. Rocca al Mare büroohoone - Audi torn (arhitekt Meeli Truu Nord Projektist); Delta Plaza (arhitektid Jüri Okas ja Marika Lõoke); Tammsaare Ärimaja (arhitekt Andres Kariste); Nordea maja (arhitektid Emil Urbel ja Indrek Erm)

  18. Effect of oral booster vaccination of rainbow trout against Yersinia ruckeri depends on type of primary immunization

    DEFF Research Database (Denmark)

    Jaafar, Rzgar M.; Al-Jubury, Azmi; Dalsgaard, Inger

    2017-01-01

    Vaccination of rainbow trout against Enteric Redmouth Disease (ERM) caused by Yersinia ruckeri can be successfully performed by administering vaccine (a bacterin consisting of formalin killed bacteria) by immersion, bath or injection. Booster immunization is known to increase the protection of fi...

  19. Läbi tantsu kasvame suureks pereks / Steven Toomela, Gert Erik ; interv. Regina Lilleorg

    Index Scriptorium Estoniae

    Toomela, Steven

    2006-01-01

    Vabariikliku noorte tantsijate X suvelaagri lõputööna etendub vabaõhumuusikal "Kaua võib" (stsenarist Rünno Karna, muusika autor ja projektijuht Kalle Erm, kunstnik Rait Potisepp, dirigent Hirvo Surva). Püünsi põhikooli tantsulapsed sellest, mis laagris meeltmööda ja mis mitte. Vastab ka laste õpetaja Hele Pomerants

  20. Rocca al Mare kool = Rocca al Mare School / Piret Lindpere

    Index Scriptorium Estoniae

    Lindpere, Piret, 1963-

    2000-01-01

    Projekteerija Arhitektuuribüroo Urbel ja Peil. Arhitektid Emil Urbel, Indrek Erm, sisekujundaja Taso Mähar. Peatöövõtt: KMG Ehitus AS. Projekt 1999, hoone valmis 2000. 23 ill. Asendiplaan, korruste plaanid, pikilõiked, sise- ja välisvaated

  1. Profiling the environmental risk management of Chinese local environmental agencies

    NARCIS (Netherlands)

    He, G.; Zhang, L.; Mol, A.P.J.; Lu, Y.

    2013-01-01

    The increasing frequency and impact of environmental accidents have pushed the issue of environmental risk management (ERM) to the top of the Chinese governments’ agendas and popularized the term ‘emergency response.’ Although the boundary between environmental accidents and other types of accidents

  2. Dicty_cDB: Contig-U10274-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 86 isolate EhV86. 46 1.8 1 ( AC193267 ) Cavia porcellus clone CH234-253D11, WORKING DRAFT... 46 1.8 1 ( FL643819 ) TS32-E12 Reticulit...ermes flavipes symbiont library... 46 1.8 1 ( AE001437 ) Clostridium acetobutylicum

  3. The external rotation method for reduction of acute anterior shoulder dislocations

    Science.gov (United States)

    Marinelli, Mario

    2009-01-01

    Background Shoulder dislocations account for almost 50% of all joint dislocations, and are most commonly anterior (90–98%) and occur due to trauma. This prospective study was conducted to report our experiences of using the external rotation method (ERM) in the reduction of acute anterior shoulder dislocation. Materials and methods Between August 2006 and April 2007, ERM was applied to 31 patients who presented with traumatic anterior shoulder dislocation to the Emergency Department of our Hospital which is a level 2 trauma centre. We evaluated the type of dislocation, the effectiveness of the procedure in achieving reduction, the need for premedication, the ease of performing the reduction, and complications, if any. Results Of the 31 patients, 29 had a successful reduction. No premedication was required in 25 patients who had a successful reduction, and the average time required for reduction in 25 was less than 2 min. Only five patients reported severe pain during the process of reduction. The method was not successful in two patients in whom the reduction was achieved under narcosis. We experienced a success rate of 89% at the first attempt. None of the patients encountered any complication. Conclusions ERM for the reduction of acute anterior dislocation of the shoulder is a safe and reliable method, mainly without requirement for any sedatives or opiate analgesics, that can be performed relatively painlessly for anterior shoulder dislocations. As no single method has a 100% success rate, ERM is a useful one to know. PMID:19384630

  4. Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes.

    Science.gov (United States)

    Yoshida, Koki; Sato, Jun; Takai, Rie; Uehara, Osamu; Kurashige, Yoshihito; Nishimura, Michiko; Chiba, Itsuo; Saitoh, Masato; Abiko, Yoshihiro

    2015-09-01

    Induced pluripotent stem (iPS) cells are generated from adult cells and are potentially of great value in regenerative medicine. Recently, it was shown that iPS cells can differentiate into ameloblast-like cells in cultures using feeder cells. In the present study, we sought to induce differentiation of ameloblast-like cells from iPS cells under feeder-free conditions using medium conditioned by cultured epithelial cell rests of Malassez (ERM) cells and gelatin-coated dishes. Two culture conditions were compared: co-cultures of iPS cells and ERM cells; and, culture of iPS cells in ERM cell-conditioned medium. Differentiation of ameloblast-like cells in the cultures was assessed using real-time RT-PCR assays of expression of the marker genes keratin 14, amelogenin, and ameloblastin and by immunocytochemical staining for amelogenin. We found greater evidence of ameloblast-like cell differentiation in the cultures using the conditioned medium. In the latter, the level of amelogenin expression increased daily and was significantly higher than controls on the 7th, 10th, and 14th days. Expression of ameloblastin also increased daily and was significantly higher than controls on the 14th day. The present study demonstrates that mouse iPS cells can be induced to differentiate into ameloblast-like cells in feeder-free cell cultures using ERM cell-conditioned medium and gelatin-coated dishes.

  5. The Gut as Reservoir of Antibiotic Resistance: Microbial Diversity of Tetracycline Resistance in Mother and Infant

    DEFF Research Database (Denmark)

    de Vries, Lisbeth Elvira; Valles, Yvonne; Agersø, Yvonne

    2011-01-01

    library, Tc-r was present exclusively in streptococci carrying tet(M), tet(L) and erm(T) within a novel composite transposon, Tn6079. This transposon belongs to a family of broad host range conjugative elements, implying a potential for the joint spread of tetracycline and erythromycin resistance within...

  6. Responses of fungal root colonization, plant cover and leaf nutrients to long-term exposure to elevated atmospheric CO2 and warming in a subarctic birch forest understory

    DEFF Research Database (Denmark)

    Olsrud, Maria; Carlsson, Bengt Å.; Svensson, Brita M.

    2010-01-01

    to the fungal symbionts. In this study, we investigated how ericoid mycorrhiza (ErM), fine endophytes (FE) and dark septate endophytes (DSE) in roots responded to elevated atmospheric CO2 concentrations and warming in the dwarf shrub understory of a birch forest in the subarctic region of northern Sweden...

  7. Ergosterol content in ericaceous hair roots correlates with dark septate endophytes but not with ericoid mycorrhizal colonization

    DEFF Research Database (Denmark)

    Olsrud, Maria; Michelsen, Anders; Wallander, Håkon

    2007-01-01

    The relationship between ergosterol content in ericaceous hair roots and ericoid mycorrhizal (ErM) colonization versus dark septate endophytic (DSE) hyphal colonization was examined in a dwarf shrub-dominated subarctic mire in Northern Sweden. Ergosterol content in hair roots did not correlate...

  8. Drug: D03591 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available g hormone receptor 2 agonist [HSA:1395] [KO:K04579] hsa04080(1394+1395) Neuroactive ligand-receptor interaction hsa04730(1394) Long-t...erm depression Anatomical Therapeutic Chemical (ATC) classification [BR:br08303] V

  9. Role of the Porphyromonas gingivalis iron-binding protein PG1777 in oxidative stress resistance.

    Science.gov (United States)

    McKenzie, Rachelle M E; Henry, Leroy G; Boutrin, Marie-Claire; Ximinies, Alexia; Fletcher, Hansel M

    2016-02-01

    Whole genome sequencing of the response of Porphyromonas gingivalis W83 to hydrogen peroxide revealed an upregulation of several uncharacterized, novel genes. Under conditions of prolonged oxidative stress in P. gingivalis, increased expression of a unique transcriptional unit carrying the grpE, dnaJ and three other hypothetical genes (PG1777, PG1778 and PG1779) was observed. The transcriptional start site of this operon appears to be located 91 bp upstream of the translational start, with a potential -10 region at -3 nt and a -35 region at -39 nt. Isogenic P. gingivalis mutants FLL273 (PG1777 : : ermF-ermAM) and FLL293 (PG1779 : : ermF-ermAM) showed increased sensitivity to and decreased survival after treatment with hydrogen peroxide. P. gingivalis FLL273 showed a fivefold increase in the formation of spontaneous mutants when compared with the parent strain after exposure to hydrogen peroxide. The recombinant PG1777 protein displayed iron-binding properties when incubated with FeSO4 and Fe(NH4)2(SO4).6H2O. The rPG1777 protein protected DNA from degradation when exposed to hydrogen peroxide in the presence of iron. Taken together, the data suggest that the grpE-dnaJ-PG1777-PG1778-PG1779 transcriptional unit may play an important role in oxidative stress resistance in P. gingivalis via its ability to protect against DNA damage.

  10. Welches Geschlecht hat die Avantgarde?

    Directory of Open Access Journals (Sweden)

    Gertrud Lehnert

    2000-07-01

    Full Text Available Das Buch betrachtet das Theater der französischen und amerikanischen ‚Avantgarde‘ der Moderne und Postmoderne unter dem Aspekt des Geschlechts: Welcher Verfahren bedient sich avantgardistische Kunst, ist avantgardistische Kunst in geschlechtlicher Hinsicht restriktiv, oder ermöglicht sie die subversive feministische oder auch lesbisch-schwule Ausformung spezifischer Formen?

  11. Uue aastatuhande puitmajad / Tiina Kolk

    Index Scriptorium Estoniae

    Kolk, Tiina

    2010-01-01

    Tutvustatakse viit kaasaegset puithoonet: puitmaja Padisel (arhitekt Indrek Allmann), eramu Kiili vallas (arhitekt Karmo Tõra), Aaviku elurajoon (arhitektid Emil Urbel, Indrek Erm), etno-modernne palkmaja Neeme külas (arhitektid Tauno Aadma, Laur Pihel), villa Saareülle Loobul (arhitekt Raul Vaiksoo)

  12. Development of a risk-based environmental management tool for drilling discharges. Summary of a four-year project

    NARCIS (Netherlands)

    Singsaas, I.; Rye, H.; Frost, T.K.; Smit, M.G.D.; Garpestad, E.

    2008-01-01

    This paper briefly summarizes the ERMS project and presents the developed model by showing results from environmental fates and risk calculations of a discharge from offshore drilling operations. The developed model calculates environmental risks for the water column and sediments resulting from

  13. Kõnnivad köiel ja hoiavad kätt / Iir Hermeliin, Jaanus Vahtra, Hille Iir Hermeliin vt. ka Ermel ; intervjueerinud Sven Karja

    Index Scriptorium Estoniae

    Iir Hermeliin, pseud., 1973-

    2000-01-01

    Vestlus Tartus teatrikunstniku Hille Ermeli (Iir Hermeliin) ning kostüümikunstniku Jaanus Vahtraga, kellel valmisid "Vanemuises" tööd lavastustele "Jacques ja tema isand" ning "Laulatus". Teatrikunstniku ametist, näitlejatest, kostüümidest, moest, lavast jm. Mati Unt J. Vahtrast, Ain Mäeots Iir Hermeliinist

  14. Experiment list: SRX150531 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatme...| cell=GM12892 || cell organism=human || cell description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedig...ree 1463, treatment: Epstein-Barr Virus transformed || c

  15. Physik für Studenten der Natur- und Technikwissenschaften

    CERN Document Server

    Stroppe, Heribert

    1999-01-01

    Dieses bewährte Lehrbuch vermittelt in kompakter Form einen Überblick über das gesamte physikalische Grundlagenwissen. Die inhaltiche Gliederung nach den Grundmodellen der Physik : Teilchen, Kontinua, Wärme, Felder, Wellen, Quanten, und die didaktische Aufbereitung des Lehrstoffes ermöglichen eine rationelle Wissensaneignung. [...

  16. Maalikunsti klassika Tallinnas müügil

    Index Scriptorium Estoniae

    2004-01-01

    Tallinna Barons Hotel'is on professor Daniele Ermes Dondé koopiate näitusmüük maailmakuulsatest meistritöödest. Professorile kuuluv firma Dondé & Partners alustas legaalse kopeerimisega 1970-ndatel aastatel. Faux Art'i (jäljenduskunst) tähendus

  17. Secondary immune response of rainbow trout following repeated immersion vaccination

    DEFF Research Database (Denmark)

    Jaafar, R. M.; Al-Jubury, Azmi; Chettri, Jiwan Kumar

    2018-01-01

    vaccination of fish raises a secondary immune response, consisting of rapid, accelerated and increased antibody reaction. This study reports how rainbow trout responds to repeated immersion vaccination against yersiniosis (ERM) caused by the bacterial pathogen Yersinia ruckeri. It was found that rainbow trout...

  18. Secondary immune response of rainbow trout following repeated immersion vaccination

    DEFF Research Database (Denmark)

    Jaafar, R. M.; Al-Jubury, A.; Chettri, J. K.

    2017-01-01

    vaccination of fish raises a secondary immune response, consisting of rapid, accelerated and increased antibody reaction. This study reports how rainbow trout responds to repeated immersion vaccination against yersiniosis (ERM) caused by the bacterial pathogen Yersinia ruckeri. It was found that rainbow trout...

  19. Edith Stein als Phänomenologin : Betrachtungen zur Phänomenologie im Grenzbereich zur Theologie

    NARCIS (Netherlands)

    Wulf, Mariéle

    2017-01-01

    Edith Steins Phänomenologie basiert auf der Person als erkennendes Subjekt. Das ermöglicht ihr, Sinnfragen zu thematisieren – bis hinein in den theologischen Bereich. Steins phänomenologische Methode, die sie aus der philosophischen Phänomenolo-gie gewonnen hat, behält sie bei, als sie sich

  20. "Mõne ne ljudi, a karmanõ" / Vitali Belobrovtsev

    Index Scriptorium Estoniae

    Belobrovtsev, Vitali, 1946-

    2004-01-01

    Rahvusvahelise tänavamööblikonkursi "Tallinna moodul" võitis inglise disainerite Clayton Welhami ja Martin Yongi töö "Fold". Linna peadisaineri Aavo Ermeli kommentaar. Tallinna tänavamööbli uuendamise rahalisest aspektist

  1. Aaviku elamurajoon Tallinnas / Piret Lindpere

    Index Scriptorium Estoniae

    Lindpere, Piret, 1963-

    2004-01-01

    Aaviku elamurajoon Jõeküla tee ja Tiskre tee vahelisel alal. Arhitektid Emil Urbel ja Indrek Erm, arendaja Tiit Nurklik. Väikevormide kontseptsiooni autorid Maria Pukk ja Ivar Lubjak. Eesti Kultuurkapitali 2003. a. arhitektuurne eripreemia. Arvamus: Epp Lankots,. Andres Kurg. 7 ill

  2. Sündinud mittetapjaks / Meelis Kapstas

    Index Scriptorium Estoniae

    Kapstas, Meelis, 1963-

    2002-01-01

    Ilmar Raagi Hamleti-teemaline lühimängufilm "Miski on mäda ehk Kogu vale Hamletist" ("Something is Rotten or All the Lie About Hamlet") võrdluses Kenneth Branagh' suurejoonelise Hollywoodi toodanguga "Hamlet" : USA 1996. Lisa : A. Ermeli koostatud K. Branagh' filmograafia lk. 86

  3. Resource efficient quality of service management for NGN services in federated cloud environments

    OpenAIRE

    Schreiner, Florian

    2015-01-01

    Seit dem Aufkommen Internet-basierter Kommunikationsdienste ist die traditionelle Telekommunikationsindustrie einer stets wachsenden Konkurrenz ausgesetzt und steht unter zunehmendem Kostendruck. Die Einführung paketvermittelnder Next Generation Network (NGN) basierter Kommunikationsinfrastrukturen ermöglicht schon heute einer Vielzahl von Netzbetreibern signifikante Kostenersparnisse durch Konsolidierung der Netzinfrastrukturen und durch Konvergenz mobiler, Festnetz-, Kabel- und Satelliten-b...

  4. Contribution of Enterococci to the Spread of Antibiotic Resistance in the Production Chain of Swine Meat Commodities

    National Research Council Canada - National Science Library

    Rizzotti, Lucia; Simeoni, Desj; Cocconcelli, Piersandro; Gazzola, Simona; Dellaglio, Franco; Torriani, Sandra

    2005-01-01

    ... and analyzed for the presence of 11 antibiotic resistance (AR) genes. Specific PCR assays carried out on DNA extracted directly from the samples revealed a high incidence of the genes tet(K) (80.5%), ermB (66.7%), and tet(M) (66.7...

  5. EDRMS for Academic Records Management: A Design Study in a Malaysian University

    Science.gov (United States)

    Miah, Shah Jahan; Samsudin, Ahmad Zam Hariro

    2017-01-01

    Higher education institutes such as universities suffer from a range of issues in managing their academic records and relevant digital contents. Many universities nowadays use specific software applications for their effective mechanism in records management. The effective provision of enterprises records management (ERM) software for managing…

  6. The Phase of Illness Paradigm: A Checklist Centric Model to Improve Patient Care in the Burn Intensive Care Unit

    Science.gov (United States)

    2015-04-01

    additional education for the healthcare team. 1. Gantt Chart Key: red line = Core Site progress; purple line = Houston Site progress; dark blue line...Checklist Centric Model to Improve Patient Care in the Burn Intensive Care Unit ERMS/Log Number: 12340054 W81XWH-13-2-0011 PI: LTC Jeremy Pamplin, MD Org

  7. Automated Information Systems Plan

    Science.gov (United States)

    1989-10-01

    XNS-TCP/IP = Xerox Network Service-Transmission Control Protocol/ Internet Protocol 35 APPENDIX A SUMMARY OF USER SURVEY RESPONSES This appendix is...COVERED 14 DATE OF REPORT (Year, Month, Day) IS PAGE COUNT Fi FROM _____ _____ October 1989 40 16 53PPEMEN-AR’Y NOTATON 7 COSA -I CODES 13 SUBECT rERMS

  8. Experiment list: SRX019964 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Barr Virus.../Search.aspx?PgId=165&q=GM06990 || cell line=GM06990 || cell type=Epstein-Barr Virus transformed lymphoblast

  9. Characterization of glycopeptide-resistant Enterococcus faecium (GRE) from broilers and pigs in Denmark: Genetic evidence that persistence of GRE in pig herds is associated with coselection by resistance to macrolides

    DEFF Research Database (Denmark)

    Aarestrup, Frank Møller

    2000-01-01

    Glycopeptide-resistant enterococci (GRE) from broilers and pigs were characterized to investigate the background for the persistence of GRE in pig herds. All porcine isolates belonged to closely related pulsed-field gel electrophoretic (PFGE) types, with the ermB and vanA genes located on the sam...

  10. Relationship between copper, glycopeptide, and macrolide resistance among Enterococcus faecium strains isolated from pigs in Denmark between 1997 and 2003

    DEFF Research Database (Denmark)

    Hasman, Henrik; Aarestrup, Frank Møller

    2005-01-01

    A significant relationship between copper resistance (tcrB), glycopeptide resistance (Tn1546), and macrolide resistance [erm(B)] in Enterococcus faecium isolated from pigs was found. The tcrB gene was located closely upstream of the Tn1546 element. However, the continued use of copper sulfate has...

  11. Electronic Resources Management Project Presentation 2012

    KAUST Repository

    Ramli, Rindra M.

    2012-11-05

    This presentation describes the electronic resources management project undertaken by the KAUST library. The objectives of this project is to migrate information from MS Sharepoint to Millennium ERM module. One of the advantages of this migration is to consolidate all electronic resources into a single and centralized location. This would allow for better information sharing among library staff.

  12. Somewhere over the Verde Rainbow

    Science.gov (United States)

    Ekart, Donna F.

    2008-01-01

    When the electronic resource management system (ERM) at Kansas State University Libraries suffered a horrible data loss, the "contract db" presented a challenge for the librarians responsible for electronic resources. It was a decent data repository, but it had no ability to manage the tangled process of licensing, acquiring, activating,…

  13. Investigating Constraint-Based Approaches for the Development of Agile Plans

    Science.gov (United States)

    2014-06-01

    as chosen du s. Three var ctivity. The rted positio o at the bou rrespondin intensity o all contribut n the type o erms of loca In TPEM...Company Cbt Tm will have to clear two routes, namely DIAM along A log team Plato Route know 4.2. The f synch are p tasks interv availa links

  14. The effect of agricultural practices on the development of indigenous arbuscular mycorrhizal fungi. I. Field studies in an Indonesian ultisol

    NARCIS (Netherlands)

    Boddington, C.L.; Dodd, J.C.

    2000-01-01

    Two pre-established agricultural field trials were assessed for the abundance of arbuscularmycorrhizal fungi (AMF) in the soil (density of spores, species richness and lengths of extra-radical mycelium [ERM]) in association with one of three tropical plant species (Gliricidia sepium, Peltophorum

  15. Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland.

    LENUS (Irish Health Repository)

    Kiely, R A

    2010-02-01

    The screening of 2000 women of childbearing age in Cork between 2004 and 2006 produced 37 erythromycin-resistant group B streptococcus (GBS) isolates. PCR analysis was performed to determine the basis for erythromycin resistance. The ermTR gene was most frequently expressed (n = 19), followed by the ermB gene (n = 8). Four isolates harboured the mefA gene. Six isolates yielded no PCR products. Some phenotype-genotype correlation was observed. All isolates expressing the mefA gene displayed the M phenotype whilst all those expressing ermB displayed the constitutive macrolide resistance (cMLS(B)) phenotype. Of 19 isolates that expressed the ermTR gene, 16 displayed the inducible macrolide resistance (iMLS(B)) phenotype. Serotype analysis revealed that serotypes III and V predominated in these isolates. The identification of two erythromycin-resistant serotype VIII isolates among this collection represents the first reported finding of erythromycin resistance in this serotype. A single isolate was non-typable using two latex agglutination serotyping kits.

  16. Experiment list: SRX080394 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah, treatment: Epstein-Ba...f=GM06990 || cell=GM06990 || cell organism=Human || cell description=Lymphoblastoid, International HapMap Pr

  17. Experiment list: SRX100458 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...ype description=Chromatin IP Sequencing || cell description=B-lymphocyte, lymphoblastoid, International HapM...ll organism=human || cell description=lymphoblastoid, International HapMap Projec

  18. Experiment list: SRX080348 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme...Detail.aspx?Ref=GM12873 || cell=GM12873 || cell organism=Human || cell description=B-Lymphocyte, Lymphoblastoid, International

  19. Experiment list: SRX080440 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme...Detail.aspx?Ref=GM12865 || cell=GM12865 || cell organism=Human || cell description=B-Lymphocyte, Lymphoblastoid, International

  20. Experiment list: SRX080405 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme...Detail.aspx?Ref=GM12874 || cell=GM12874 || cell organism=Human || cell description=B-Lymphocyte, Lymphoblastoid, International

  1. Experiment list: SRX080428 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme...Detail.aspx?Ref=GM12872 || cell=GM12872 || cell organism=Human || cell description=B-Lymphocyte, Lymphoblastoid, International

  2. Experiment list: SRX080387 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme..._Detail.aspx?Ref=GM12875 || cell=GM12875 || cell organism=Human || cell description=B-Lymphocyte, Lymphoblastoid, International

  3. In vitro activity of josamycin against Streptococcus pyogenes isolated from patients with upper respiratory tract infections in France.

    Science.gov (United States)

    Auzou, M; Caillon, J; Poyart, C; Weber, P; Ploy, M-C; Leclercq, R; Cattoir, V

    2015-07-01

    The primary objective of our study was to obtain susceptibility data for josamycin against Streptococcus pyogenes isolated from patients presenting with upper respiratory tract infections in France. The secondary objective was to characterize the molecular mechanism of resistance in macrolide-resistant isolates. MICs of erythromycin, clarithromycin, azithromycin, josamycin, and clindamycin were determined by the broth microdilution method. Resistance genes erm(B), erm(TR), and mef(A) were screened by PCR. The MIC50 and MIC90 of josamycin against 193 isolates of S. pyogenes were 0.12 and 0.25mg/L, respectively, with a resistance rate estimated at 4.7%. Resistance was due to the erm(B) gene whereas strains harboring erm(TR) or mef(A) remained susceptible. Josamycin was active against >95% of S. pyogenes isolated from patients with upper respiratory tract infections, and can be used as an alternative for the treatment of pharyngitis. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  4. Eramu Suurupis = A House in Suurupi / Tiina Mang

    Index Scriptorium Estoniae

    Mang, Tiina

    2002-01-01

    2002. a. valminud eramu projekteeris Arhitektibüroo Emil Urbel. Arhitektid Emil Urbel ja Indrek Erm, sisearhitekt Tiina Mang, konstruktor Peep Rosenberg. Projekt 2000-2001. 7 ill.: I korruse plaan, sise- ja välisvaated.Emil Urbel (arhitektibüroo).

  5. Piiripealne privaatsus : ridaelamu Esku tänaval Tallinnas = Bordeline Privacy : Row-House on Esku Street in Tallinn / Emil Urbel ; üles. kirjut. Triin Ojari

    Index Scriptorium Estoniae

    Urbel, Emil, 1959-

    2004-01-01

    Tüüpmajast, eramajade ise-projekteerimisest ja -ehitamisest, 12-kortelisest puitlaudisega ridaelamust, privaatsuse piiridest. Ridaelamu projekteerija: AB Emil Urbel. Arhitektid Emil Urbel ja Indrek Erm, konstruktor Tõnu Peipman. Projekt 2003, valmis 2004. Ill.: I, II ja III korruse plaan, 6 värv. välisvaadet.

  6. AcEST: BP911773 [AcEST

    Lifescience Database Archive (English)

    Full Text Available RM_STRSA rRNA adenine N-6-methyltransferase OS=Streptococcus sanguis Align length 37 Score (bit) 32.0 E-valu...h... 30 8.0 >sp|P06573|ERM_STRSA rRNA adenine N-6-methyltransferase OS=Streptococcus sanguis PE=3 SV=1 Lengt

  7. Möglichkeiten und Grenzen bausteinorientierter Anforderungsmanagement-Prozessverbesserung in KMUs

    OpenAIRE

    Adam, S.; Doerr, J.; Eisenbarth, M.

    2006-01-01

    Traditionelle Prozessverbesserungsinstrumente wie CMMI oder SPICE sind aufgrund mangelndem finanziellen und zeitlichen Spielraum oft nicht von KMUs ein- bzw. umzusetzen. Eine Alternative bieten bausteinorientierte Ansätze wie beispielsweise ReqMan, die eine flexible und dennoch problemgenaue Verbesserung ermöglichen. Dieser Erfahrungsbericht beleuchtet die Ergebnisse aus drei industriellen Fallstudien hinsichtlich der Anwendung der ReqMan-Methode.

  8. The Asian financial crisis : identification, interdependence, and transmission

    NARCIS (Netherlands)

    Lestano, M.

    2006-01-01

    The 1980s and 1990s witnessed several episodes of financial turmoil, including the sovereign debt crisis of Latin America in the 1980s, the near-breakdown of the European Exchange Rate Mechanism (ERM) in 1992-1993 and the severe crisis that swept through Asian economies in 1997-1998. Three

  9. Experiment list: SRX080417 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1459, treatme...Detail.aspx?Ref=GM12864 || cell=GM12864 || cell organism=Human || cell description=B-Lymphocyte, Lymphoblastoid, International

  10. Experiment list: SRX100481 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available erm|Description=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion,...type description=Chromatin IP Sequencing || cell description=B-lymphocyte, lymphoblastoid, International Hap...ell organism=human || cell description=lymphoblastoid, International HapMap Proje

  11. On the Matlis duals of local cohomology modules and modules of ...

    Indian Academy of Sciences (India)

    ,. a a proper ideal of R and M a finitely generated R-module with aM = M. Let D(−) := HomR(−,E) be the Matlis dual functor, where E := E(R/m) is the injective hull of the residue field R/m. In this paper, by using a complex which involves modules ...

  12. Meeting the Challenges of Enterprise Risk Management in Higher Education

    Science.gov (United States)

    Mattie, John

    2007-01-01

    In the summer of 2007, the Association of Governing Boards of Universities and Colleges (AGB) and the National Association of College and University Business Officers (NACUBO) held a summit on enterprise risk management (ERM) in Washington DC with senior officers and trustees from several leading colleges and universities. Approximately 40…

  13. The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis.

    Science.gov (United States)

    Juda, Marek; Chudzik-Rzad, Beata; Malm, Anna

    2016-03-01

    Coagulase-negative staphylococci, particularly Staphylococcus epidermidis, can be regarded as potential reservoirs of resistance genes for pathogenic strains, e.g., Staphylococcus aureus. The aim of this study was to assess the prevalence of different resistance phenotypes to macrolide, lincosamide, and streptogramins B (MLSB) antibiotics among erythromycin-resistant S. epidermidis, together with the evaluation of genes promoting the following different types of MLSB resistance:ermA, ermB, ermC,msrA, mphC, and linA/A'. Susceptibility to spiramycin was also examined. Among 75 erythromycin-resistantS. epidermidis isolates, the most frequent phenotypes were macrolides and streptogramins B (MSB) and constitutive MLSB (cMLSB). Moreover, all strains with the cMLSB phenotype and the majority of inducible MLSB (iMLSB) isolates were resistant to spiramycin, whereas strains with the MSB phenotype were sensitive to this antibiotic. The D-shape zone of inhibition around the clindamycin disc near the spiramycin disc was found for some spiramycin-resistant strains with the iMLSB phenotype, suggesting an induction of resistance to clindamycin by this 16-membered macrolide. The most frequently isolated gene was ermC, irrespective of the MLSB resistance phenotype, whereas the most often noted gene combination wasermC, mphC, linA/A'. The results obtained showed that the genes responsible for different mechanisms of MLSB resistance in S. epidermidis generally coexist, often without the phenotypic expression of each of them.

  14. Evolution and origin of merlin, the product of the Neurofibromatosis type 2 (NF2 tumor-suppressor gene

    Directory of Open Access Journals (Sweden)

    Omelyanchuk Leonid V

    2005-12-01

    Full Text Available Abstract Background Merlin, the product of the Neurofibromatosis type 2 (NF2 tumor suppressor gene, belongs to the ezrin-radixin-moesin (ERM subgroup of the protein 4.1 superfamily, which links cell surface glycoproteins to the actin cytoskeleton. While merlin's functional activity has been examined in mammalian and Drosophila models, little is understood about its evolution, diversity, and overall distribution among different taxa. Results By combining bioinformatic and phylogenetic approaches, we demonstrate that merlin homologs are present across a wide range of metazoan lineages. While the phylogenetic tree shows a monophyletic origin of the ERM family, the origin of the merlin proteins is robustly separated from that of the ERM proteins. The derivation of merlin is thought to be in early metazoa. We have also observed the expansion of the ERM-like proteins within the vertebrate clade, which occurred after its separation from Urochordata (Ciona intestinalis. Amino acid sequence alignment reveals the absence of an actin-binding site in the C-terminal region of all merlin proteins from various species but the presence of a conserved internal binding site in the N-terminal domain of the merlin and ERM proteins. In addition, a more conserved pattern of amino acid residues is found in the region containing the so-called "Blue Box," although some amino acid substitutions in this region exist in the merlin sequences of worms, fish, and Ciona. Examination of sequence variability at functionally significant sites, including the serine-518 residue, the phosphorylation of which modulates merlin's intra-molecular association and function as a tumor suppressor, identifies several potentially important sites that are conserved among all merlin proteins but divergent in the ERM proteins. Secondary structure prediction reveals the presence of a conserved α-helical domain in the central to C-terminal region of the merlin proteins of various species. The

  15. Soluble form of LR11 is highly increased in the vitreous fluids of patients with idiopathic epiretinal membrane.

    Science.gov (United States)

    Hashimoto, Ryuya; Jiang, Meizi; Shiba, Tomoaki; Hiruta, Nobuyuki; Takahashi, Mao; Higashi, Morihiro; Hori, Yuichi; Bujo, Hideaki; Maeno, Takatoshi

    2017-05-01

    LR11 (also called SorLA or SORL1) is a migration regulator of adherent cells with the immature proliferative phenotype. The present study investigated the clinical and pathological involvement of the soluble form of LR11 (sLR11) in the idiopathic epiretinal membrane (iERM). The subjects were 51 patients with iERM (24 cellophane macular reflex (CMR) and 27 preretinal macular fibrosis (PMF)) and 45 patients with macular holes as age and sex-matched controls. Vitreous sLR11 and transforming growth factor (TGF)β2 levels were measured by ELISA. The sLR11 levels in the vitreous fluids of patients with iERM (20.2 ± 8.1 ng/mL) were significantly higher than those in controls (11.4 ± 4.7 ng/mL). Among the patients with iERM, the vitreous sLR11 levels were significantly higher in PMF (23.6 ± 8.2 ng/mL), than those in CMR (16.5 ± 5.9 ng/mL). Multivariate regression analysis of the studied factors showed that sLR11 was a unique factor independently contributing to the discrimination of the iERM patients against the control subjects (odds ratio [OR] 1.35 per 1-ng/mL increase, 95% CI 1.09-1.67; P = 0.004). ROC analysis showed that the sensitivity and the specificity of sLR11, but not of other studied factors, categorized into the rank of moderate accuracy. Finally, there was a positive correlation (R = 0.588; P = 0.003) between the vitreous levels of sLR11 and TGFβ2 using the available samples. sLR11 levels in vitreous fluids were specifically increased in patients with iERM, suggesting the involvement in the pathology of proliferative and migrating cells for the development of iERM.

  16. Measurement of the spin structure of the neutron using polarised deep inelastic scattering

    Science.gov (United States)

    Kaiser, Ralf Bernd

    The measurement of the spin structure function g1p of the proton and its integral Γ1p by the EMC experiment at C scERN in 1988 indicated that only 12% ± 17% of the proton spin is carried by quarks. This unexpected result-the so called 'spin crisis'-lead to a series of new experimental proposals. One of these, the H scERMES experiment, uses the polarised positron beam of the H scERA accelerator together with a polarised internal gas target of hydrogen, deuterium or 3He for the study of the spin structure of the nucleon. The scattered positrons and other products of the reaction are detected in a forward spectrometer with large acceptance. This thesis focuses on three topics, after a review of the relevant theory and an overview of the H scERMES experiment: The H scERMES transition radiation detector (TRD), which is used to distinguish high energy positrons from hadrons, the H scERMES particle identification (PID) system and the measurement of the spin structure function g1n of the neutron. The H scERMES TRD is the main Canadian contribution to the apparatus of the experiment. The H scERMES PID system allows the identification of positrons from deep inelastic scattering with an efficiency of 99% and a hadron contamination of less than 0.5%. The first physics result from the 1995 H scERMES data is the measurement of the spin structure function g1n(x) of the neutron. The value of the resulting integral Γ1n=∫01g1n(x)/ dx confirms previous measurements at SLAC and violates the Ellis-Jaffe sum rule by about one sigma. The contribution of the quarks to the spin of the neutron can be calculated in the framework of the quark parton model to be 37 ± 16%, indicating that less than half of the spin of the neutron is carried by quarks.

  17. Effects of rapid maxillary expansion with six months of retention and no further orthodontic treatment

    Directory of Open Access Journals (Sweden)

    Kelly Regina Micheletti

    2016-06-01

    Full Text Available A expansão rápida ou lenta da maxila seguida do uso de aparelhos ortodônticos fixos altera a face e os dentes. Este estudo avaliou as mudanças dentoesqueléticas após a expansão rápida da maxila (ERM e determinou a estabilidade dessas mudanças em três anos de acompanhamento. Dez crianças com mordida cruzada posterior bilateral foram avaliadas (grupo experimental. Modelos de estudo e telerradiografias laterais foram avaliadas antes da ERM (T0, assim como três meses (T1, um ano (T2 e três anos (T3 após a ERM. As medidas foram comparadas as de um grupo controle compostos de 21 pacientes por meio do teste ANOVA e teste t Student (p > 0.05. As mudanças no plano transversal revelaram uma expansão significante no arco superior três meses após a ERM, que se manteve estável após três anos. A expansão ao nível das cúspides foi similar à do nível gengival. Mas nenhuma diferença significante foi encontrada estre as medidas cefalométricas angulares e lineares ao longo dos períodos T0, T1, T2, e T3 e nem quando se comparou essas medidas as do grupo controle (p > 0.05. Conclui-se que A ERM precoce produz aumentos transversais estáveis no arco superior sem alterar significantemente as relações sagitais e verticais dentoesqueléticas e nem inclinar dentes.

  18. Os efeitos da expansão rápida da maxila sobre a permeabilidade nasal avaliados por rinomanometria e rinometria acústica The effects of rapid maxillary expansion on the nasal patency assessed by rhinomanometry and acoustic rhinometry

    Directory of Open Access Journals (Sweden)

    Bruno Alberto Vidotti

    2008-12-01

    Full Text Available O objetivo deste artigo foi analisar os achados da literatura acerca das repercussões da expansão rápida da maxila (ERM sobre a permeabilidade nasal, pela rinomanometria, que avalia a resistência oferecida pela cavidade nasal à passagem do fluxo aéreo, e pela rinometria acústica, que mede as áreas seccionais e os volumes de diferentes segmentos da cavidade nasal. Estudos que utilizaram a rinomanometria evidenciaram que a ERM determina uma diminuição significativa da resistência nasal, assim como o aumento da área de secção transversa mínima nasal (ASTM em parcela significativa de pacientes. Estudos com rinometria acústica mostraram que a ERM determina, adicionalmente, um aumento significativo do volume da cavidade nasal anterior. Entretanto, é consenso que a ERM não se justifica, por si, como forma de induzir um padrão nasal de respiração em respiradores bucais habituais, apesar dos benefícios trazidos pela ERM em termos de permeabilidade nasal.The aim of this review was to analyze the findings reported in the literature about the effects of rapid maxillary expansion (RME on nasal patency assessed by rhinomanometry, which measures the resistance offered by the nasal cavity to airflow, and acoustic rhinometry, which measures sectional areas and volumes of different segments of the nasal cavity. Studies using rhinomanometry have demonstrated a significant decrease of nasal resistance after RME on a significant number of patients, and also an increase in minimal nasal cross-sectional area. Studies using acoustic rhinometry have additionally shown a significant increase of anterior nasal volume. However, it is a consensus that RME is not justified only for the purpose of inducing a nasal pattern of breathing in oral breathers, despite the improvement of nasal patency caused by RME.

  19. Effects of bamboo charcoal on antibiotic resistance genes during chicken manure composting.

    Science.gov (United States)

    Li, Haichao; Duan, Manli; Gu, Jie; Zhang, Yajun; Qian, Xun; Ma, Jun; Zhang, Ranran; Wang, Xiaojuan

    2017-06-01

    Composting is widely used for animal waste disposal, and bamboo charcoal (BC) can be used for nitrogen conservation during composting. However, the effects of BC on antibiotic resistance genes (ARGs) during chicken manure composting are still unclear. This study investigated the effects on ARGs of adding different proportions of BC (0%, 5%, 10%, and 20% w/w) to chicken manure compost. After 26 days, the relative abundances (RAs) of most ARGs (tetC, tetG, tetW, tetX, sul2, drfA1, drfA7, ermB, ermF, ermQ, and ermX) and intI1 declined by 21.6-99.5%, whereas sul1 increased by 7.5-17.7 times. The average RAs reductions with 0%, 5%, 10%, and 20% BC were 0.85, 1.05, 1.08, and 1.15 logs, respectively. The most important environmental factor for the ARG profiles was temperature according to redundancy analysis. Furthermore, BC significantly decreased the bio-Cu and bio-Zn levels, thereby reducing the co-selection pressure from heavy metals. Different proportions of BC had no significant effects on the removal of tetG, tetW, tetX, sul2, drfA1, and ermB. Supplementation with 10% BC was more effective at removing tetC and drfA7 compared with the other treatments. The results suggested that 10% BC supplementation is appropriate for reducing ARGs in chicken manure compost. Copyright © 2017 Elsevier Inc. All rights reserved.

  20. Dental follicle cells and cementoblasts induce apoptosis of ameloblast-lineage and Hertwig's epithelial root sheath/epithelial rests of Malassez cells through the Fas-Fas ligand pathway.

    Science.gov (United States)

    Lee, Ji-Hyun; Lee, Dong-Seol; Nam, Hyun; Lee, Gene; Seo, Byoung-Moo; Cho, Young-Sik; Bae, Hyun-Sook; Park, Joo-Cheol

    2012-02-01

    Hertwig's epithelial root sheath (HERS), epithelial rests of Malassez (ERM) cells, and reduced ameloblasts undergo apoptosis during tooth development. This study examined the effects of dental follicle cells and cementoblasts on the apoptosis of ameloblast-lineage and HERS/ERM cells derived from the enamel organ. We also elucidated the induction pathways and identified the apoptotic pathway involved in this process. Here, we showed terminal deoxynucleotidyl transferase-mediated biotin-dUTP nick-end labeling (TUNEL)-positive HERS cells and reduced ameloblasts near dental follicle cells during tooth development. Co-culturing ameloblast-lineage cell line (ALC) ameloblasts and HERS/ERM cells with either dental follicle cells or OCCM-30 cementoblasts markedly enhanced the apoptosis of ameloblasts and HERS/ERM cells compared with cells cultured alone. However, dental follicle cells and cementoblasts did not modulate the apoptotic responses of co-cultured non-odontogenic MCF10A or KB cells. When ameloblasts + HERS and cementoblasts + dental follicle cells were co-cultured, the expression of Fas ligand (FasL) increased in cementoblasts + dental follicle cells, while the expression of Fas increased in ameloblasts + HERS. Interestingly, recombinant FasL induced ameloblast apoptosis while the cementoblast-induced ameloblast apoptosis was suppressed by the Fas/FasL antagonist Kp7-6. These results suggest that during tooth development, dental follicle cells and cementoblasts induce apoptosis of ameloblast-lineage and HERS/ERM cells through the Fas-FasL pathway, but do not induce the apoptosis of non-odontogenic epithelial cells. © 2011 Eur J Oral Sci.

  1. Quantification of changes in foveal capillary architecture caused by idiopathic epiretinal membrane using OCT angiography.

    Science.gov (United States)

    Nelis, P; Alten, F; Clemens, C R; Heiduschka, P; Eter, N

    2017-07-01

    To quantify the extent and depth of distortion of the foveal capillary architecture due to traction of an idiopathic epiretinal membrane (ERM) using optical coherence tomography angiography (OCT-A). Multimodal imaging including OCT-A (Angiovue, Optovue) was performed in 42 eyes with idiopathic ERM (72.4 years ±6.8). Best corrected visual acuity (BCVA), OCT-A vessel density of the foveal (VDfo) and parafoveal (VDp) region were assessed. Based on 6 × 6-mm 2 OCT-A images, a macular vessel density ratio (MVR = VDfo/VDp) was calculated for the superficial (s), deep (d) and full-thickness (f) slabs to assess a depth-resolved, non-invasive evaluation of foveal distortion. The acquired data were subdivided in a patient group with mild and significant BCVA reduction due to ERM. Data was compared to age-matched healthy controls. In all three slabs, MVR was significantly smaller in the control group in comparison with the ERM group: MVRs: 0.63 ± 0.1 vs 0.83 ± 0.1 (p > 0.001); MVRd: 0.60 ± 0.1 vs 0.73 ± 0.1 (p  = 0.4 LogMar) showed a significantly higher MVR in all three slabs: MVRs: 0.64 ± 0.1 vs 0.86 ± 0.1 (p architecture due to traction of ERM. BCVA reduction appears to be associated with extent and depth of distortion.

  2. The Potential of Dark Septate Endophytes to Form Root Symbioses with Ectomycorrhizal and Ericoid Mycorrhizal Middle European Forest Plants

    Science.gov (United States)

    Lukešová, Tereza; Kohout, Petr; Větrovský, Tomáš; Vohník, Martin

    2015-01-01

    The unresolved ecophysiological significance of Dark Septate Endophytes (DSE) may be in part due to existence of morphologically indistinguishable cryptic species in the most common Phialocephala fortinii s. l.—Acephala applanata species complex (PAC). We inoculated three middle European forest plants (European blueberry, Norway spruce and silver birch) with 16 strains of eight PAC cryptic species and other DSE and ectomycorrhizal/ericoid mycorrhizal fungi and focused on intraradical structures possibly representing interfaces for plant-fungus nutrient transfer and on host growth response. The PAC species Acephala applanata simultaneously formed structures resembling ericoid mycorrhiza (ErM) and DSE microsclerotia in blueberry. A. macrosclerotiorum, a close relative to PAC, formed ectomycorrhizae with spruce but not with birch, and structures resembling ErM in blueberry. Phialocephala glacialis, another close relative to PAC, formed structures resembling ErM in blueberry. In blueberry, six PAC strains significantly decreased dry shoot biomass compared to ErM control. In birch, one A. macrosclerotiorum strain increased root biomass and the other shoot biomass in comparison with non-inoculated control. The dual mycorrhizal ability of A. macrosclerotiorum suggested that it may form mycorrhizal links between Ericaceae and Pinaceae. However, we were unable to detect this species in Ericaceae roots growing in a forest with presence of A. macrosclerotiorum ectomycorrhizae. Nevertheless, the diversity of Ericaceae mycobionts was high (380 OTUs) with individual sites often dominated by hitherto unreported helotialean and chaetothyrialean/verrucarialean species; in contrast, typical ErM fungi were either absent or low in abundance. Some DSE apparently have a potential to form mycorrhizae with typical middle European forest plants. However, except A. applanata, the tested representatives of all hitherto described PAC cryptic species formed typical DSE colonization without

  3. Short-term chromium (VI) exposure increases phosphorus uptake by the extraradical mycelium of the arbuscular mycorrhizal fungus Rhizophagus irregularis MUCL 41833.

    Science.gov (United States)

    Gil-Cardeza, María Lourdes; Calonne-Salmon, Maryline; Gómez, Elena; Declerck, Stéphane

    2017-11-01

    Hexavalent chromium is a potent carcinogen, while phosphorus is an essential nutrient. The role of arbuscular mycorrhizal fungi (AMF) in the uptake of P is well known and was also reported, at low levels, for Cr. However, it is unclear whether the uptake of Cr can impact the short-term uptake dynamics of P since both elements have a similar chemical structure and may thus potentially compete with each other during the uptake process. This study investigated the impact of Cr(VI) on short-term P uptake by the AMF Rhizophagus irregularis MUCL 41833 in Medicago truncatula. Bi-compartmented Petri plates were used to spatially separate a root compartment (RC) from a hyphal compartment (HC) using a whole plant in vitro culture system. The HC was supplemented with Cr(VI). Chromium(VI) as well as total Cr and P were monitored during 16 h within the HC and their concentrations determined by the end of the experiment within roots and shoots. Our results indicated that the uptake and translocation of Cr from hyphae to roots was a fast process: roots in which the extraradical mycelium (ERM) was exposed to Cr(VI) accumulated more Cr than roots of which the ERM was not exposed to Cr(VI) or was dead. Our results further confirmed that dead ERM immobilized more Cr than alive ERM. Finally our results demonstrated that the short exposure to Cr(VI) was sufficient to stimulate P uptake by the ERM and that the stimulation process began within the first 4 h of exposure. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Global 3D Imaging of Yersinia ruckeri Bacterin Uptake in Rainbow Trout Fry

    Science.gov (United States)

    Ohtani, Maki; Villumsen, Kasper Rømer; Koppang, Erling Olaf; Raida, Martin Kristian

    2015-01-01

    Yersinia ruckeri is the causative agent of enteric redmouth disease (ERM) in rainbow trout, and the first commercially available fish vaccine was an immersion vaccine against ERM consisting of Y. ruckeri bacterin. The ERM immersion vaccine has been successfully used in aquaculture farming of salmonids for more than 35 years. The gills and the gastrointestinal (GI) tract are believed to be the portals of antigen uptake during waterborne vaccination against ERM; however, the actual sites of bacterin uptake are only partly understood. In order to obtain insight into bacterin uptake during waterborne vaccination, optical projection tomography (OPT) together with immunohistochemistry (IHC) was applied to visualize bacterin uptake and processing in whole rainbow trout fry. Visualization by OPT revealed that the bacterin was initially taken up via gill lamellae from within 30 seconds post vaccination. Later, bacterin uptake was detected on other mucosal surfaces such as skin and olfactory bulb from 5 to 30 minutes post vaccination. The GI tract was found to be filled with a complex of bacterin and mucus at 3 hours post vaccination and the bacterin remained in the GI tract for at least 24 hours. Large amounts of bacterin were present in the blood, and an accumulation of bacterin was found in filtering lymphoid organs such as spleen and trunk kidney where the bacterin accumulates 24 hours post vaccination as demonstrated by OPT and IHC. These results suggest that bacterin is taken up via the gill epithelium in the earliest phases of the bath exposure and from the GI tract in the later phase. The bacterin then enters the blood circulatory system, after which it is filtered by spleen and trunk kidney, before finally accumulating in lymphoid organs where adaptive immunity against ERM is likely to develop. PMID:25658600

  5. In vitro antimicrobial susceptibility and genetic resistance determinants of Streptococcus agalactiae isolated from mastitic cows in Brazilian dairy herds

    Directory of Open Access Journals (Sweden)

    Juliana Rosa da Silva

    2017-08-01

    Full Text Available Streptococcus agalactiae is one of the main causative agents of bovine mastitis and is associated with several economic losses for producers. Few studies have evaluated antimicrobial susceptibility and the prevalence of genetic resistance determinants among isolates of this bacterium from Brazilian dairy cattle. This work aimed to evaluate the frequency of the antimicrobial resistance genes ermA, ermB, mefA, tetO, tetM, aphA3, and aad-6, and in vitro susceptibility to the antimicrobials amikacin, erythromycin, clindamycin, tetracycline, gentamicin, penicillin, ceftiofur, and cefalotin, and the associations between resistance genotypes and phenotypes among 118 S. agalactiae isolates obtained from mastitic cows in Brazilian dairy herds. Of the resistance genes examined, ermB was found in 19 isolates (16.1%, tetO in 23 (19.5%, and tetM in 24 (20.3%. The genes ermA, mefA, aphA3, and aad-6 were not identified. There was an association between the presence of genes ermB, tetM, and tetO and phenotypic resistance to erythromycin, clindamycin, and tetracycline. Rates of resistance to the tested antibiotics varied, as follows: erythromycin (19.5%, tetracycline (35.6%, gentamicin (9.3%, clindamycin (20.3%, penicillin (3.4%, and amikacin (38.1%; conversely, all isolates were susceptible to ceftiofur and cefalotin. Antimicrobial resistance testing facilitates the treatment decision process, allowing the most judicious choice of antibiotics. Moreover, it enables regional and temporal monitoring of the resistance dynamics of this pathogen of high importance to human and animal health.

  6. Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year period

    Directory of Open Access Journals (Sweden)

    Trevor W. Alexander

    2013-10-01

    Full Text Available Mannheimia haemolytica isolated from feedlot cattle were tested for tulathromycin resistance. Cattle were sampled over a three-year period, starting 12 months after approval of tulathromycin for prevention and treatment of bovine respiratory disease. Nasopharyngeal samples from approximately 5,814 cattle were collected when cattle entered feedlots (N = 4 and again from the same cattle after ≥ 60 d on feed. The antimicrobial use history for each animal was recorded. Mannheimia haemolytica was isolated from 796 (13.7% entry samples and 1,038 (20.6% ≥ 60 d samples. Of the cattle positive for M. haemolytica, 18.5%, 2.9%, and 2.4% were administered therapeutic concentrations of tulathromycin, tilmicosin, or tylosin tartrate, respectively. In addition, 13.2% were administered subtherapeutic concentrations of tylosin phosphate in feed. In years one and two, no tulathromycin-resistant M. haemolytica were detected, whereas 5 isolates (0.4% were resistant in year three. These resistant isolates were collected from three cattle originating from a single pen, were all serotype 1, and were genetically related (≥ 89% similarity according to pulsed-field gel electrophoreses patterns. The five tulathromycin-resistant isolates were multi-drug resistant also exhibiting resistance to oxytetracycline, tilmicosin, ampicillin, or penicillin. The macrolide resistance genes erm(42, erm(A, erm(B, erm(F, erm(X and msr(E-mph(E, were not detected in the tulathromycin-resistant M. haemolytica. This study showed that tulathromycin resistance in M. haemolytica from a general population of feedlot cattle in western Canada was low and did not change over a three-year period after tulathromycin was approved for use in cattle.

  7. Effect of Serial Intrasilicone Oil Bevacizumab Injections in Eyes With Recurrent Proliferative Vitreoretinopathy Retinal Detachment.

    Science.gov (United States)

    Hsu, Jason; Khan, M Ali; Shieh, Wen-Shi; Chiang, Allen; Maguire, Joseph I; Park, Carl H; Garg, Sunir J; Ho, Allen C; Kaiser, Richard S

    2016-01-01

    To investigate the effect of serial intrasilicone oil bevacizumab injections (1.25 mg/0.05 mL) on visual acuity (VA) and anatomic outcomes in eyes undergoing proliferative vitreoretinopathy (PVR)-related retinal detachment (RD) repair. Prospective, nonrandomized, historical-control pilot study. setting: Tertiary care center. Nondiabetic eyes undergoing pars plana vitrectomy (PPV) and silicone oil tamponade with or without scleral buckling procedure (SBP) for recurrent RD due to PVR. Intrasilicone oil injection of 1.25 mg bevacizumab was performed intraoperatively and at postoperative months 1, 2, and 3. Retinal reattachment rate, final VA, and rate of epiretinal membrane (ERM) formation at month 6. Twenty eyes of 20 patients were enrolled and compared to a historical control group composed of 35 age- and sex-matched controls. In the study group, logMAR VA improved from mean 1.78 ± 0.43 (Snellen 20/1205) to 1.43 ± 0.70 (Snellen 20/538, P = .04), retinal reattachment was achieved in 14 of 20 eyes (70%), and ERM formation was observed in 7 of 20 eyes (35%) at 6 months. In the control group, logMAR VA improved from mean 1.50 ± 0.74 (Snellen 20/632) to 1.43 ± 0.58 (Snellen 20/538, P = .64), retinal reattachment was achieved in 25 of 35 eyes (71%), and ERM formation was observed in 7 of 35 eyes (20%) at 6 months. No significant difference in final VA (P = .96), retinal reattachment rate (P = .75), or ERM formation (P = .33) was observed between groups. No intrasilicone oil injection-related adverse events occurred. Serial intrasilicone oil injections of bevacizumab did not improve retinal reattachment rate, improve final VA, or reduce ERM formation in patients undergoing PVR-related RD surgery. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Correlations between M-CHARTS and PHP findings and subjective perception of metamorphopsia in patients with macular diseases.

    Science.gov (United States)

    Arimura, Eiko; Matsumoto, Chota; Nomoto, Hiroki; Hashimoto, Shigeki; Takada, Sonoko; Okuyama, Sachiko; Shimomura, Yoshikazu

    2011-01-05

    To assess the correlations between a patient's subjective perception of metamorphopsia and the clinical measurements of metamorphopsia by M-CHARTS and PreView PHP (PHP). The authors designed a 10-item questionnaire focusing on the symptoms of metamorphopsia and verified its validity with a Rasch analysis. M-CHARTS measured the minimum visual angle of a dotted line needed to detect metamorphopsia, and PHP used the hyperacuity function for detection. Subjects were 39 patients with idiopathic epiretinal membrane (ERM), 22 patients with idiopathic macular hole (M-hole), 19 patients with age-related macular degeneration (AMD), and 51 healthy controls. Rasch analysis suggested the elimination of one question. The nine-item questionnaire score significantly correlated to the M-CHARTS score in ERM (r = 0.59; P = 0.0004) but not in M-hole and to the PHP result in AMD (r = -0.29; P = 0.04) but not in ERM. Eighty percent of ERM patients with greater horizontal M-CHARTS score subjectively perceived horizontal metamorphopsia more often. M-CHARTS showed better sensitivities than PHP in both ERM (89% vs. 42%) and AMD (74% vs. 68%) and better specificity (100% vs. 71%) in healthy controls. Rasch analysis indicated that the present form of the questionnaire is better suited for moderate to severe cases of metamorphopsia than for mild cases. The questionnaire appears to be a valid assessment of patient subjective perception of metamorphopsia and can be used to supplement the clinical measurements of metamorphopsia by M-CHARTS and PHP in patients with macular diseases.

  9. The potential of Dark Septate Endophytes to form root symbioses with ectomycorrhizal and ericoid mycorrhizal middle European forest plants.

    Directory of Open Access Journals (Sweden)

    Tereza Lukešová

    Full Text Available The unresolved ecophysiological significance of Dark Septate Endophytes (DSE may be in part due to existence of morphologically indistinguishable cryptic species in the most common Phialocephala fortinii s. l.--Acephala applanata species complex (PAC. We inoculated three middle European forest plants (European blueberry, Norway spruce and silver birch with 16 strains of eight PAC cryptic species and other DSE and ectomycorrhizal/ericoid mycorrhizal fungi and focused on intraradical structures possibly representing interfaces for plant-fungus nutrient transfer and on host growth response. The PAC species Acephala applanata simultaneously formed structures resembling ericoid mycorrhiza (ErM and DSE microsclerotia in blueberry. A. macrosclerotiorum, a close relative to PAC, formed ectomycorrhizae with spruce but not with birch, and structures resembling ErM in blueberry. Phialocephala glacialis, another close relative to PAC, formed structures resembling ErM in blueberry. In blueberry, six PAC strains significantly decreased dry shoot biomass compared to ErM control. In birch, one A. macrosclerotiorum strain increased root biomass and the other shoot biomass in comparison with non-inoculated control. The dual mycorrhizal ability of A. macrosclerotiorum suggested that it may form mycorrhizal links between Ericaceae and Pinaceae. However, we were unable to detect this species in Ericaceae roots growing in a forest with presence of A. macrosclerotiorum ectomycorrhizae. Nevertheless, the diversity of Ericaceae mycobionts was high (380 OTUs with individual sites often dominated by hitherto unreported helotialean and chaetothyrialean/verrucarialean species; in contrast, typical ErM fungi were either absent or low in abundance. Some DSE apparently have a potential to form mycorrhizae with typical middle European forest plants. However, except A. applanata, the tested representatives of all hitherto described PAC cryptic species formed typical DSE

  10. An in vivo evaluation of Brilliant Blue G in animals and humans.

    Science.gov (United States)

    Remy, M; Thaler, S; Schumann, R G; May, C A; Fiedorowicz, M; Schuettauf, F; Grüterich, M; Priglinger, S G; Nentwich, M M; Kampik, A; Haritoglou, C

    2008-08-01

    To evaluate the retinal toxicity of Brilliant Blue G (BBG) following intravitreal injection in rat eyes and examine the biocompatibility and the staining properties in humans. BBG was injected into the 11 rat eyes to evaluate toxic effects with balanced salt solution (BSS) serving as control. Retinal toxicity was assessed by retinal ganglion cell (RGC) counts and by light microscopy 7 days later. In addition, BBG was applied during vitrectomy for macular hole (MH) (n = 15) or epiretinal membranes (ERM) (n = 3) in a prospective, non-comparative consecutive series of patients. Before and after surgery, all patients underwent a complete clinical examination including measurement of best corrected visual acuity (VA) and intraocular pressure, perimetry, fundus photography and optical coherence tomography. Patients were seen 1 day before surgery and then in approximately four weeks intervals. No significant reduction in RGC numbers and no morphological alterations were noted. A sufficient staining of the internal limiting membrane (ILM) was seen in patients with MH, while the staining pattern in ERM cases was patchy, indicating that parts of the ILM were peeled off along with the ERM in a variable extent. All MHs could be closed successfully. VA improved in 10 eyes (56%; 8/15 MH patients, 2/3 ERM patients), was unchanged in four eyes (22%; all MH patients) and was reduced in four eyes (22%; 3/15 MH, 1/3 ERM). No toxic effects attributable to the dye were noted during patient follow-up. The ultrastructure of tissue harvested during surgery was unremarkable. Brilliant Blue provides a sufficient and selective staining of the ILM. No retinal toxicity or adverse effects related to the dye were observed in animal and human studies. The long-term safety of this novel dye will have to be evaluated in larger patient series and a longer follow-up.

  11. Characterization of methicillin-resistant Staphylococcus aureus isolated from healthy turkeys and broilers using DNA microarrays

    Directory of Open Access Journals (Sweden)

    Hosny El-Adawy

    2016-12-01

    Full Text Available Methicillin-resistant Staphylococcus aureus (MRSA is a major human health problem and recently, domestic animals, in particular pigs and poultry are discussed as carriers and possible reservoirs of MRSA. Twenty seven S. aureus isolates from five turkey farms (n=18 and two broiler farms (n=9 were obtained by culturing of choana and skin swabs from apparently healthy birds, identified by Taqman-based real-time duplex nuc-mecA-PCR and characterized by spa typing as well as by a DNA microarray based assay which covered, amongst others, a considerable number of antibiotic resistance genes, species controls and virulence markers. The antimicrobial susceptibility profiles were tested by agar diffusion assays and genotypically confirmed by the microarray. Five different spa types (3 in turkeys and 2 in broilers were detected. The majority of MRSA isolates (24/27 belonged to clonal complex 398-MRSA-V. The most frequently occurring spa types were accordingly t011, t034 and t899. A single CC5-MRSA-III isolated from turkey and CC398-MRSA with an unidentified/truncated SCCmec element in turkey and broiler were additionally detected. The phenotypic antimicrobial resistance profiles of S. aureus isolated from both turkeys and broilers against 14 different antimicrobials showed that all isolates were resistant to ampicillin, cefoxitin, oxacillin, doxycycline and tetracycline. Moreover, all S. aureus isolated from broilers were resistant to erythromycin and azithromycin. All isolates were susceptible to gentamicin, chloramphenicol, sulphonamides and fusidic acid. The resistance rate against ciprofloxacin was 55.6% in broiler isolates and 42.1% in turkey isolates. All tetracycline resistant isolates possessed genes tetK/M. All erythromycin-resistant broiler isolates carried ermA. Only one broiler isolate (11.1% carried genes ermA, ermB and ermC, while 55.6% of turkey isolates possessed ermA and ermB genes.Neither PVL genes (lukF/S-PV, animal-associated leukocidin

  12. Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Healthy Turkeys and Broilers Using DNA Microarrays.

    Science.gov (United States)

    El-Adawy, Hosny; Ahmed, Marwa; Hotzel, Helmut; Monecke, Stefan; Schulz, Jochen; Hartung, Joerg; Ehricht, Ralf; Neubauer, Heinrich; Hafez, Hafez M

    2016-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) is a major human health problem and recently, domestic animals are described as carriers and possible reservoirs. Twenty seven S. aureus isolates from five turkey farms (n = 18) and two broiler farms (n = 9) were obtained by culturing of choana and skin swabs from apparently healthy birds, identified by Taqman-based real-time duplex nuc-mecA-PCR and characterized by spa typing as well as by a DNA microarray based assay which covered, amongst others, a considerable number of antibiotic resistance genes, species controls, and virulence markers. The antimicrobial susceptibility profiles were tested by agar diffusion assays and genotypically confirmed by the microarray. Five different spa types (3 in turkeys and 2 in broilers) were detected. The majority of MRSA isolates (24/27) belonged to clonal complex 398-MRSA-V. The most frequently occurring spa types were accordingly t011, t034, and t899. A single CC5-MRSA-III isolated from turkey and CC398-MRSA with an unidentified/truncated SCCmec element in turkey and broiler were additionally detected. The phenotypic antimicrobial resistance profiles of S. aureus isolated from both turkeys and broilers against 14 different antimicrobials showed that all isolates were resistant to ampicillin, cefoxitin, oxacillin, doxycycline, and tetracycline. Moreover, all S. aureus isolated from broilers were resistant to erythromycin and azithromycin. All isolates were susceptible to gentamicin, chloramphenicol, sulphonamides, and fusidic acid. The resistance rate against ciprofloxacin was 55.6% in broiler isolates and 42.1% in turkey isolates. All tetracycline resistant isolates possessed genes tetK/M. All erythromycin-resistant broiler isolates carried ermA. Only one broiler isolate (11.1%) carried genes ermA, ermB, and ermC, while 55.6% of turkey isolates possessed ermA and ermB genes. Neither PVL genes (lukF/S-PV), animal-associated leukocidin (lukM and luk-P83) nor the gene encoding

  13. Modeling gross primary production and ecosystem respiration for terrestrial ecosystems in North China and Tibet Plateau using MODIS imagery

    Science.gov (United States)

    Gao, Y.; Yu, G.; Yan, H.; Zhu, X.; Li, S.; Wang, Q.; Zhang, J.; Wang, Y.; Li, Y.; Zhao, L.; Shi, P.

    2013-12-01

    Gross primary production (GPP) and ecosystem respiration (Re) are two large components in the studying of regional and global carbon cycles. Accurate quantification of spatio-temporal variations of GPP and Re for terrestrial ecosystems is of great importance to research carbon budget on regional and global scales. In this study, we proposed two satellite-based models, i.e. Photosynthetic Capacity Model (PCM) and Ecosystem Respiration Model (ERM), to simulate GPP and Re of terrestrial ecosystems, respectively. Multi-year eddy CO2 flux data from five vegetation types in North China (temperate mixed forest, temperate steppe) and Tibet Plateau (alpine shrubland, alpine marsh and alpine meadow-steppe) were used for assessing the model performances. The PCM model was driven by the Enhanced Vegetation Index (EVI) and the Land Surface Water Index (LSWI) from MODIS imagery. In most cases, the PCM-simulated GPP and the observed GPP displayed very consistent seasonal and inter-seasonal variability regardless of vegetation types. The PCM predicted versus observed GPP performed better than the MODIS GPP products, and was compatible with the Vegetation Photosynthesis Model (VPM). Moreover, the model parameter of the PCM could be gained from the linear function of mean annual remote sensing data. Based on this linear function, the PCM model simulated 93% variations of the observed GPP across all five vegetation types. The ERM model was developed based on both GPP and temperature, and was driven by EVI, LSWI and the Land Surface Temperature (LST) from MODIS imagery. In most cases, the seasonal and interannual variations of the simulated Re matched well with the observed Re. Compared with the model driven by temperature, and the model further added GPP in the reference respiration, the ERM model was optimal in each vegetation type. The model parameters of the ERM could also be presented by the liner functions of mean annual remote sensing data. Based on these linear functions, 90

  14. Does Oral Vaccination Protect Rainbow Trout (Oncorhynchus mykiss) Against Enteric Red Mouth Disease?

    DEFF Research Database (Denmark)

    Neumann, Lukas; Villumsen, Kasper Rømer; Kragelund Strøm, Helene

    The effect of oral vaccines against bacterial fish diseases has been a topic for debate in many years. Recently both M-cells and dendritic cells have been found in fish and it is therefore likely that antigens can be taken up from the intestine and induce immunity in orally vaccinated fish....... The objective for this project is to investigate whether oral vaccination of rainbow trout against Yersinia ruckeri O1 (biotype 1) causing Enteric Red Mouth disease (ERM) can protect rainbow trout against a subsequent experimental bath challenge with Y. ruckeri. The rainbow trout were given oral vaccinations...... with AquaVacTM ERM Oral vet. (MSD animal health) or an experimental vaccine based on killed Yersinia ruckeri O1, (biotype 1) bacteria. Seven groups were studied: 1) Control group (no vaccination, no infection), 2) infected control, 3) experimental vaccine, 4) experimental vaccine w/ booster (4 months post...

  15. Oral and Anal vaccination against enteric red mouth disease protection against yersiniosis

    DEFF Research Database (Denmark)

    Neumann, Lukas; Villumsen, Kasper Rømer; Kragelund Strøm, Helene

    The effect of oral vaccines against bacterial fish diseases has been a topic for debate in many years. Recently both M-cells and dendritic cells have been found in fish and it is therefore likely that antigens can be taken up from the intestine and induce immunity in orally and anally vaccinated...... fish. The objective for this project is to investigate whether oral and anal vaccination of rainbow trout against Yersinia ruckeri O1 (biotype 1) causing Enteric Red Mouth disease (ERM) can protect rainbow trout against a subsequent experimental bath challenge.The rainbow trout were given oral...... vaccinations with AquaVacTM ERM Oral vet. (MSD animal health) or an experimental vaccine based on formalin killed Yersinia ruckeri O1, (biotype 1) bacteria. Eight groups were studied: 1) Control group (no vaccination, no infection), 2) infected control, 3) experimental vaccine, 4) experimental vaccine w...

  16. A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

    Science.gov (United States)

    Veréb, Zoltán; Facskó, Andrea; Hawlina, Marko

    2014-01-01

    A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment. PMID:25276840

  17. A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

    Directory of Open Access Journals (Sweden)

    Sofija Andjelic

    2014-01-01

    Full Text Available A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs from cataract surgery and proliferative diabetic retinopathy (PDR fibrovascular epiretinal membranes (fvERMs from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.

  18. A Livestock-Associated, Multidrug-Resistant, Methicillin-Resistant Staphylococcus aureus Clonal Complex 97 Lineage Spreading in Dairy Cattle and Pigs in Italy

    DEFF Research Database (Denmark)

    Feltrin, Fabiola; Alba, Patricia; Kraushaar, Britta

    2016-01-01

    Pandemic methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 97 (CC97) lineages originated from livestock-to-human host jumps. In recent years, CC97 has become one of the major MRSA lineages detected in Italian farmed animals. The aim of this study was to characterize and analyze...... by macrorestriction pulsed-field gel electrophoresis (PFGE) analysis, multilocus sequence typing (MLST), spa typing, staphylococcal cassette chromosome mec (SCCmec) typing, and antimicrobial resistance pattern analysis. Virulence and resistance genes were investigated by PCR and microarray analysis. Most...... Panton-Valentine leukocidin (PVL) negative, and a few (n = 7) tested positive for sak or scn. All MRSA isolates were multidrug resistant (MDR), and the main features were erm(B)- or erm(C)-mediated (n = 18) macrolide-lincosamide-streptogramin B resistance, vga(A)-mediated (n = 37) pleuromutilin...

  19. Avaliação do efeito da expansão rápida da maxila na via aérea superior, por meio da nasofibroscopia: descrição da técnica e relato de caso

    OpenAIRE

    Jorge,Edmilsson Pedro; Santos-Pinto,Ary dos; Gandini Júnior,Luiz Gonzaga; Guariza Filho,Odilon; Castro,Anibal Benedito Batista Arrais Torres de

    2011-01-01

    A finalidade deste artigo é avaliar o efeito da expansão rápida da maxila (ERM) na via aérea superior. Por intermédio de um caso clínico, será relatado como indivíduos com atresia da maxila e com comprometimento da função naso-respiratória podem beneficiar-se com a ERM. Para entender melhor as alterações morfológicas decorrentes do paciente com problemas respiratórios, deve-se conhecer a anatomia e a fisiologia do sistema respiratório. Entretanto, não se pode esquecer que o tratamento deste p...

  20. Instability-related delamination growth in thermoset and thermoplastic composites

    Science.gov (United States)

    Gillespie, John W., Jr.; Carlsson, Leif A.; Rothschilds, Robert J.

    1988-01-01

    Mixed-mode crack propagation in compressively loaded thermoset and thermoplastic composite columns with an imbedded through-width delamination is investigated. Beam theory is used to analyze the geometrically nonlinear load-deformation relationship of the delaminated subregion. The elastic restraint model (ERM), combined with existing FSM modeling of the crack-tip region, yields expressions for the Mode I and Mode II components of the strain energy release rate G(I) and G(II) to predict the critical load at the onset of delamination growth. Experimental data were generated for geometries yielding a wide range of G(I)/G(II) ratios at the onset of crack growth. A linear mixed-mode crack growth criterion in conjunctuion with the ERM provides good agreement between predicted and measured critical loads for both materials studied.

  1. Comparative protection of two different commercial vaccines against Yersinia ruckeri serotype O1 and biotype 2 in rainbow trout (Oncorhynchus mykiss)

    DEFF Research Database (Denmark)

    Deshmukh, S.; Raida, M. K.; Dalsgaard, Inger

    2012-01-01

    Differentially extended specific protection by two commercial vaccines against Yersinia ruckeri serotype O1 biotype 2 was studied following 30s immersion exposure. Rainbow trout were challenged intra-peritoneally (i.p.) with Y. ruckeri serotype O1, biotype 2 (≈106 to 107CFU/fish) at 4, 6 and 8...... months after vaccination with vaccines containing either biotype 1 (AquaVac® ERM) or both biotypes 1 and 2 (AquaVac® RELERA™). The specific pattern of vaccine-mediated protection was evaluated by relative percentage survival (RPS) analysis at 4 and 6 months post-vaccination and by obtaining gross...... months without any booster vaccination. A specific and adaptive response induced by AquaVac® RELERA™ vaccine against Y. ruckeri biotype 2 was clearly indicated. In addition, some degree of cross protection rendered by AquaVac® ERM containing biotype 1 during infection with Y. ruckeri biotype 2 was also...

  2. Fate of antibiotic resistance genes and its drivers during anaerobic co-digestion of food waste and sewage sludge based on microwave pretreatment.

    Science.gov (United States)

    Zhang, Junya; Chen, Meixue; Sui, Qianwen; Wang, Rui; Tong, Juan; Wei, Yuansong

    2016-10-01

    In this study, anaerobic digestion of mono-SS, MW-SS:FW and SS:MW-FW was investigated to understand the fate of ARGs and its drivers. Anaerobic digestion was effective for the reduction of metal resistance genes (MRGs), and could reduce the abundance of blaOXA-1, sulI and tetG, while sulII in co-digestion and blaTEM and ereA only in MW-SS. ARGs reduction could be partly attributed to the reduction of co-selective pressure from heavy metals reflected by MRGs. However, the abundance of mefA/E, ermB, ermF, tetM and tetX increased significantly. Anaerobic co-digestion, especially for MW-SS, could reduce total ARGs abundance compared with mono-SS, and evolution of bacterial community was the main driver for the fate of ARGs. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Spectral-domain optical coherence tomography findings of the macula in 500 consecutive patients with uveitis.

    Science.gov (United States)

    Grajewski, R S; Boelke, A C; Adler, W; Meyer, S; Caramoy, A; Kirchhof, B; Cursiefen, C; Heindl, L M

    2016-11-01

    PurposeTo analyze the macular structure in a large series of consecutive patients with different types of uveitis using spectral-domain optical coherence tomography (SD-OCT).Patients and methodsFive hundred eyes of 500 consecutive patients with anterior, intermediate, posterior, and panuveitis underwent standardized macular examination using SD-OCT. Central retinal thickness (CRT), macular volume (MV), and presence of cystoid macular edema (CME), diffuse macular edema (DME), serous retinal detachment (SRD), epiretinal membrane with (ERM+) and without (ERM-) retinal surface wrinkling were determined.ResultsThe anatomic location of inflammation affected significantly CRT and MV (Pmacula is recommended for all uveitis patients. CRT, MV, and the incidence of CME were highest in intermediate and panuveitis.

  4. PAX3-FKHR sensitizes human alveolar rhabdomyosarcoma cells to camptothecin-mediated growth inhibition and apoptosis

    Science.gov (United States)

    Zeng, Fu-Yue; Cui, Jimmy; Liu, Lingling; Chen, Taosheng

    2009-01-01

    Patients with alveolar rhabdomyosarcoma (ARMS) have poorer response to conventional chemotherapy and lower survival rates than those with embryonal RMS (ERMS). By high-throughput screening, we identified camptothecin as an ARMS-selective inhibitor. Camptothecin more efficiently inhibited proliferation and induced apoptosis in Rh30 (ARMS) than RD (ERMS) cells. Ectopic expression of the PAX3-FKHR (PF) fusion protein in RD cells significantly increased sensitivity, whereas siRNA knockdown of PF decreased sensitivity of Rh30 cells to camptothecin. The sensitization required a transcriptionally active PF, and camptothecin downregulated levels of PF protein. These findings suggest that it is feasible to develop agents that preferentially block the growth of ARMS. PMID:19442434

  5. Steueroasen und Regulierungsoasen – Auswirkungen auf die Stabilität des Finanzmarkts und politische Implikationen

    Directory of Open Access Journals (Sweden)

    Michaela Schmidt

    2012-12-01

    Full Text Available In diesem Beitrag wird dargelegt, dass Steuer- und Regulierungsoasen nicht nur Steuerausfälle und Kapitalflucht ermöglichen, sondern auch eine Hauptursache für die Finanzkrise 2007/2008 waren und weiterhin die Stabilität des Finanzmarkts gefährden. Sie haben den Boden bereitet für das Aufkommen nicht regulierter Finanzinstitutionen, sogenannter Schattenbanken. Regulierungsoasen ermöglichen Finanzmarktakteuren eine Umgehung von Regulierungs- und Aufsichtsvorschriften und heizen den Wettbewerb um niedrige Regulierungsvorschriften maßgeblich an. Jeder Schritt zu einer finanzpolitischen Re-Regulierung von Steuer- und Regulierungsoasen sowie Schattenbanken ist ein aktiver Schritt zur Krisenvorbeugung und damit zum Schutz der ArbeitnehmerInnen vor den gewaltigen volkswirtschaftlichen Kosten von Finanzkrisen.

  6. Deferral of surgery for epiretinal membranes

    DEFF Research Database (Denmark)

    Kofod, Mads; Christensen, Ulrik C; Dornonville de la Cour, Morten

    2016-01-01

    BACKGROUND/AIMS: To compare the visual outcome in early versus deferred surgery in patients with idiopathic epiretinal membrane (ERM) and good presenting visual acuity and mild symptoms. METHODS: This study is a randomised clinical trial. 53 eyes of 53 patients with symptomatic ERM and best......-corrected visual acuity (BCVA) at presentation ≥65 early treatment diabetic retinopathy study (ETDRS) letters were randomised to immediate surgery (20 eyes) or to watchful waiting (33 eyes) for 1 year. Primary end-point was BCVA after 12 months. Secondary end-points were central macular thickness and the number...... of patients that crossed over from the watchful waiting arm to the surgery arm due to deteriorated symptoms. RESULTS: Mean BCVA after 12 months in the watchful waiting group (81 letters) were not significantly different from patients undergoing immediate surgery (82.5 letters, p=0.647). During 1 year...

  7. Retinal Changes Induced by Epiretinal Tangential Forces

    Directory of Open Access Journals (Sweden)

    Mario R. Romano

    2015-01-01

    Full Text Available Two kinds of forces are active in vitreoretinal traction diseases: tangential and anterior-posterior forces. However, tangential forces are less characterized and classified in literature compared to the anterior-posterior ones. Tangential epiretinal forces are mainly due to anomalous posterior vitreous detachment (PVD, vitreoschisis, vitreopapillary adhesion (VPA, and epiretinal membranes (ERMs. Anomalous PVD plays a key role in the formation of the tangential vectorial forces on the retinal surface as consequence of gel liquefaction (synchysis without sufficient and fast vitreous dehiscence at the vitreoretinal interface. The anomalous and persistent adherence of the posterior hyaloid to the retina can lead to vitreomacular/vitreopapillary adhesion or to a formation of avascular fibrocellular tissue (ERM resulting from the proliferation and transdifferentiation of hyalocytes resident in the cortical vitreous remnants after vitreoschisis. The right interpretation of the forces involved in the epiretinal tangential tractions helps in a better definition of diagnosis, progression, prognosis, and surgical outcomes of vitreomacular interfaces.

  8. EMDLAB: A toolbox for analysis of single-trial EEG dynamics using empirical mode decomposition.

    Science.gov (United States)

    Al-Subari, K; Al-Baddai, S; Tomé, A M; Goldhacker, M; Faltermeier, R; Lang, E W

    2015-09-30

    Empirical mode decomposition (EMD) is an empirical data decomposition technique. Recently there is growing interest in applying EMD in the biomedical field. EMDLAB is an extensible plug-in for the EEGLAB toolbox, which is an open software environment for electrophysiological data analysis. EMDLAB can be used to perform, easily and effectively, four common types of EMD: plain EMD, ensemble EMD (EEMD), weighted sliding EMD (wSEMD) and multivariate EMD (MEMD) on EEG data. In addition, EMDLAB is a user-friendly toolbox and closely implemented in the EEGLAB toolbox. EMDLAB gains an advantage over other open-source toolboxes by exploiting the advantageous visualization capabilities of EEGLAB for extracted intrinsic mode functions (IMFs) and Event-Related Modes (ERMs) of the signal. EMDLAB is a reliable, efficient, and automated solution for extracting and visualizing the extracted IMFs and ERMs by EMD algorithms in EEG study. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Risk management for companies focused on innovation processes

    Directory of Open Access Journals (Sweden)

    Ana Paula Beck da Silva Etges

    Full Text Available Abstract Risk is inherent to the activities of technology and innovation companies and to manage them represent an opportunity to improve the company capability to achieve its goals. The use of ERM models has been studied since the Committee of Sponsoring Organizations of the Treadway Commission guides. This article adapted the MIGGRI model for the context of an innovation company from a TSP in Brazil. Using a case study and a review from previous ERM literature, the article show that is possible to measure the risks that an innovation company faces, and that they may be managed with a view to supporting a company’s strategy. Were applied an economic analysis based on a MCS and an indicator of CFaR were applied to measure innovation risks. A strategic performance model for innovation companies are proposed and the benefit to implement Risk Management practices in innovation organizations was validated.

  10. Developing the ISCO Technology Practices Manual: The SERDP/ESTCP ISCO Initiative

    Science.gov (United States)

    2010-12-01

    Technical Advisory Committee (TAC) was created with experts in the field to advise the projects TAC Members Richard Brown/ERM Michael Marley /Xpert Design...Future Directions Robert Siegrist 5:00 PM Adjourn In Situ Chemical Oxidation: 2. Overview of Principles and Practices Bob Siegrist Robert L. Siegrist...ISCO Protocol Flow In Situ Chemical Oxidation: 6. CDISCO – Conceptual Design of Permanganate Injection Systems Bob Borden Robert C. Borden, Ph.D

  11. semen by MTT reduction assay

    African Journals Online (AJOL)

    user

    74. 76. 0. 0.2. 0.4. 0.6. 0.8. 1. 1.2. Rate of reduction of MTT (OD at 550nm). % o. f v iab le sp erm ato zo a. Figure 2 Relationship between MTT reduction rate and percentage of viable sperm as determined by eosin and nigrosin staining. The regression curve shown is y = 77.15-10.15x; r =- 0.32; n = 20; OD = optical density.

  12. Improving SPAWAR PEO C4I Organizational Alignment to Better Enable Enterprise Technical Risk Management

    Science.gov (United States)

    2017-03-01

    ENABLE ENTERPRISE TECHNICAL RISK MANAGEMENT Steven C. Crosson Civilian, Department of the Navy B.C.E., University of Delaware, 2004 M.S...Defense Acquisition University DOD Department of Defense ERM enterprise risk management ETR enterprise technical risk ETRB Enterprise Technical...emphasis that there are even graduate programs offering master’s degrees in enterprise risk management showing up at American universities such as

  13. Entwicklung eines exergiebasierten Analysemodells zum umfassenden Technologievergleich von Wärmeversorgungssystemen unter Berücksichtigung des Einflusses einer veränderlichen Außentemperatur

    OpenAIRE

    Bargel, Stefan

    2011-01-01

    Auf die Niedertemperaturbedarfsformen Raumwärme und Trinkwarmwasser entfallen in Deutschland ca. 30% des Endenergiebedarfs. Für diese Art von Energieströmen ist eine exergetische Analyse besonders interessant. Im Rahmen der Arbeit wurde ein Analysemodell entwickelt, das einen umfassenden Quervergleich von heute gängigen technischen Systemen zur Wärmeversorgung aus exergetischer Sicht ermöglicht. Dabei werden Kesselanlagen, KWK, Abwärmenutzung und Wärmepumpen betrachtet. Durch S...

  14. Development of Biocompatibility Procedures for Assessment of Plant Growth in Ground Test Hardware for the EMCS

    Science.gov (United States)

    Bowman, R. N.; Steele, M. K.; Sun, S. (Technical Monitor)

    2002-01-01

    The European Modular Cultivation System (EMCS) is an European Space Agency-developed facility designed to support plant research in microgravity on the IS NASA is responsible for providing US specific hardware to use within the EMCS. In preparation for flight, research will be developed and tested at Ames Research Center in the EMCS ground test hardware, the Experiment Reference Module (ERM) In order to determine the acceptability of the ERM for such purposes, biocompatibility tests will be performed to determine that the hardware functions as intended and successfully supports the' growth of plants. In this report, we describe the development of procedures and the collection of baseline data against which to compare ERM function, e.g. biocompatibility testing. A simple and robust system was developed to grow whole Arabidopsis thaliana plants within the confined volumes characteristic of spaceflight hardware. Our system for growing plants eliminated the necessity of a water/nutrient delivery system and allowed for quantifiable assessment of individual plants, as well as entire population dynamics. To insure uniform germination, seeds were started in small straw segments and transplanted into modified scintillation vials. Seedlings were selected prior to transplantation to decrease genetic variability. Plants were grown for a total of 24 days in standard laboratory plant growth chambers under controlled conditions. Sequential digital still images were taken on a daily basis. Analysis of these images allowed for the quantification of even minute environmental effect, on growth dynamics whole plants. The data collected provide reliable growth curves against which to compare plants grown in the ERM.

  15. Die Conjoint-Analyse: eine Einführung in das Verfahren mit einem Ausblick auf mögliche sozialwissenschaftliche Anwendungen

    OpenAIRE

    Klein, Markus

    2002-01-01

    'Die Conjoint-Analyse ermöglicht die Dekomposition globaler Präferenzurteile. Sie hatbislang nur in der empirischen Marktforschung größere Verbreitung gefunden. Es wirdeine kurze Einführung in das Verfahren und ein Ausblick auf mögliche Anwendungen inder empirischen Sozialforschung gegeben.' (Autorenreferat) 'Conjoint-Analysis enables the researcher to decompose global preference judgments. It isprimarily used in the field of marketing research. A short introduction to Conjoint-Analysisis ...

  16. Conjoint-Analyse und Marktsegmentierung

    OpenAIRE

    Steiner, Winfried J.; Baumgartner, Bernhard

    2003-01-01

    Die Marktsegmentierung zählt neben der Neuproduktplanung und Preisgestaltung zu den wesentlichen Einsatzgebieten der Conjoint-Analyse. Neben traditionell eingesetzten zweistufigen Vorgehensweisen, bei denen Conjoint-Analyse und Segmentierung in zwei getrennten Schritten erfolgen, stehen heute mit Methoden wie der Clusterwise Regression oder Mixture-Modellen neuere Entwicklungen, die eine simultane Segmentierung und Präferenzschätzung ermöglichen, zur Verfügung. Der Beitrag gibt einen Überblic...

  17. Methode zur Verbesserung der Usability durch gezielte Förderung mentaler Modelle

    OpenAIRE

    Jenke, Marcus; Binder, Karoline; Maier, Thomas

    2017-01-01

    Das Potenzial mentaler Modelle zur Entwicklung benutzergerechter, technischer Systeme wurde bereits in zahlreichen Studien aufgezeigt. Jedoch existieren noch immer nur wenige konkrete Methoden zur Nutzung mentaler Modelle im Zuge der Produktentwicklung. Diese Arbeit stellt daher einen konkreten Methodenansatz vor, anhand dessen es Entwicklern ermöglicht werden kann, Optimierungspotenziale mit Hilfe der mentalen Modelle zu identifizieren und diese zu beeinflussen. Dazu werden verschiedene Werk...

  18. Ionenunterstützte Antimon-Dotierung für die Silizium-Molekularstrahlepitaxie von Bauelementstrukturen

    OpenAIRE

    Eifler, Georg

    2005-01-01

    Die Molekularstrahlepitaxie (MBE) ist eine geeignete Methode zur Herstellung von aktuellen Höchstfrequenz-Bauelementstrukturen, wie dem Silizium-Germanium-Heterobipolartransistor (SiGe-HBT) mit einer ultradünnen (< 20 nm), hochdotierten Basis. Die MBE ermöglicht eine starke Reduzierung der Wachstumstemperatur, um Dotier- und Heterostrukturprofile mit Nanometer-Abmessungen zu erzeugen. In diesem Niedrigtemperatur-Bereich mit vernachlässigbarer Volumendiffusion zeigt sich, dass auch die Entmisc...

  19. Zugänglichkeit virtueller Klassenzimmer für Blinde

    OpenAIRE

    Köhlmann, Wiebke

    2016-01-01

    Die vorliegende Arbeit untersucht die Zugänglichkeit virtueller Klassenzimmer für blinde Nutzende, um eine möglichst gleichberechtigte Teilhabe an synchronen, kollaborativen Lernszenarien zu ermöglichen. Im Rahmen einer Produktanalyse werden dazu virtuelle Klassenzimmer auf ihre Zugänglichkeit und bestehende Barrieren untersucht und Richtlinien für die zugängliche Gestaltung von virtuellen Klassenzimmern definiert. Anschliessend wird ein alternatives Benutzungskonzept zur Darstellung und Be...

  20. Search for Anomalous $ZZ\\gamma$ and $Z\\gamma\\gamma$ couplings in the process $e^{+}e^{-} \\to Z\\gamma$ at LEP

    CERN Document Server

    Acciarri, M.; Adriani, O.; Aguilar-Benitez, M.; Alcaraz, J.; Alemanni, G.; Allaby, J.; Aloisio, A.; Alviggi, M.G.; Ambrosi, G.; Anderhub, H.; Andreev, Valery P.; Angelescu, T.; Anselmo, F.; Arefev, A.; Azemoon, T.; Aziz, T.; Bagnaia, P.; Bajo, A.; Baksay, L.; Balandras, A.; Baldew, S.V.; Banerjee, S.; Banerjee, Sw.; Barczyk, A.; Barillere, R.; Barone, L.; Bartalini, P.; Basile, M.; Battiston, R.; Bay, A.; Becattini, F.; Becker, U.; Behner, F.; Bellucci, L.; Berbeco, R.; Berdugo, J.; Berges, P.; Bertucci, B.; Betev, B.L.; Bhattacharya, S.; Biasini, M.; Biland, A.; Blaising, J.J.; Blyth, S.C.; Bobbink, G.J.; Bohm, A.; Boldizsar, L.; Borgia, B.; Bourilkov, D.; Bourquin, M.; Braccini, S.; Branson, J.G.; Brigljevic, V.; Brochu, F.; Buffini, A.; Buijs, A.; Burger, J.D.; Burger, W.J.; Cai, X.D.; Campanelli, Mario; Capell, M.; Cara Romeo, G.; Carlino, G.; Cartacci, A.M.; Casaus, J.; Castellini, G.; Cavallari, F.; Cavallo, N.; Cecchi, C.; Cerrada, M.; Cesaroni, F.; Chamizo, M.; Chang, Y.H.; Chaturvedi, U.K.; Chemarin, M.; Chen, A.; Chen, G.; Chen, G.M.; Chen, H.F.; Chen, H.S.; Chiefari, G.; Cifarelli, L.; Cindolo, F.; Civinini, C.; Clare, I.; Clare, R.; Coignet, G.; Colino, N.; Costantini, S.; Cotorobai, F.; de la Cruz, B.; Csilling, A.; Cucciarelli, S.; Dai, T.S.; van Dalen, J.A.; D'Alessandro, R.; de Asmundis, R.; Deglon, P.; Degre, A.; Deiters, K.; della Volpe, D.; Delmeire, E.; Denes, P.; DeNotaristefani, F.; De Salvo, A.; Diemoz, M.; Dierckxsens, M.; van Dierendonck, D.; Di Lodovico, F.; Dionisi, C.; Dittmar, M.; Dominguez, A.; Doria, A.; Dova, M.T.; Duchesneau, D.; Dufournaud, D.; Duinker, P.; Duran, I.; El Mamouni, H.; Engler, A.; Eppling, F.J.; Erne, F.C.; Extermann, P.; Fabre, M.; Faccini, R.; Falagan, M.A.; Falciano, S.; Favara, A.; Fay, J.; Fedin, O.; Felcini, M.; Ferguson, T.; Ferroni, F.; Fesefeldt, H.; Fiandrini, E.; Field, J.H.; Filthaut, F.; Fisher, P.H.; Fisk, I.; Forconi, G.; Freudenreich, K.; Furetta, C.; Galaktionov, Iouri; Ganguli, S.N.; Garcia-Abia, Pablo; Gataullin, M.; Gau, S.S.; Gentile, S.; Gheordanescu, N.; Giagu, S.; Gong, Z.F.; Grenier, Gerald Jean; Grimm, O.; Gruenewald, M.W.; Guida, M.; van Gulik, R.; Gupta, V.K.; Gurtu, A.; Gutay, L.J.; Haas, D.; Hasan, A.; Hatzifotiadou, D.; Hebbeker, T.; Herve, Alain; Hidas, P.; Hirschfelder, J.; Hofer, H.; Holzner, G.; Hoorani, H.; Hou, S.R.; Hu, Y.; Iashvili, I.; Jin, B.N.; Jones, Lawrence W.; de Jong, P.; Josa-Mutuberria, I.; Khan, R.A.; Kaur, M.; Kienzle-Focacci, M.N.; Kim, D.; Kim, J.K.; Kirkby, Jasper; Kiss, D.; Kittel, W.; Klimentov, A.; Konig, A.C.; Kopp, A.; Koutsenko, V.; Kraber, M.; Kraemer, R.W.; Krenz, W.; Kruger, A.; Kunin, A.; Ladron de Guevara, P.; Laktineh, I.; Landi, G.; Lassila-Perini, K.; Lebeau, M.; Lebedev, A.; Lebrun, P.; Lecomte, P.; Lecoq, P.; Le Coultre, P.; Lee, H.J.; Le Goff, J.M.; Leiste, R.; Leonardi, Emanuele; Levtchenko, P.; Li, C.; Likhoded, S.; Lin, C.H.; Lin, W.T.; Linde, F.L.; Lista, L.; Liu, Z.A.; Lohmann, W.; Longo, E.; Lu, Y.S.; Lubelsmeyer, K.; Luci, C.; Luckey, David; Lugnier, L.; Luminari, L.; Lustermann, W.; Ma, W.G.; Maity, M.; Malgeri, L.; Malinin, A.; Mana, C.; Mangeol, D.; Mans, J.; Marchesini, P.; Marian, G.; Martin, J.P.; Marzano, F.; Mazumdar, K.; McNeil, R.R.; Mele, S.; Merola, L.; Meschini, M.; Metzger, W.J.; von der Mey, M.; Mihul, A.; Milcent, H.; Mirabelli, G.; Mnich, J.; Mohanty, G.B.; Molnar, P.; Moulik, T.; Muanza, G.S.; Muijs, A.J.M.; Musicar, B.; Musy, M.; Napolitano, M.; Nessi-Tedaldi, F.; Newman, H.; Niessen, T.; Nisati, A.; Kluge, Hannelies; Organtini, G.; Oulianov, A.; Palomares, C.; Pandoulas, D.; Paoletti, S.; Paolucci, P.; Paramatti, R.; Park, H.K.; Park, I.H.; Passaleva, G.; Patricelli, S.; Paul, Thomas Cantzon; Pauluzzi, M.; Paus, C.; Pauss, F.; Pedace, M.; Pensotti, S.; Perret-Gallix, D.; Petersen, B.; Piccolo, D.; Pierella, F.; Pieri, M.; Piroue, P.A.; Pistolesi, E.; Plyaskin, V.; Pohl, M.; Pojidaev, V.; Postema, H.; Pothier, J.; Prokofev, D.O.; Prokofev, D.; Quartieri, J.; Rahal-Callot, G.; Rahaman, M.A.; Raics, P.; Raja, N.; Ramelli, R.; Rancoita, P.G.; Raspereza, A.; Raven, G.; Razis, P.; Ren, D.; Rescigno, M.; Reucroft, S.; Riemann, S.; Riles, Keith; Robohm, A.; Rodin, J.; Roe, B.P.; Romero, L.; Rosca, A.; Rosier-Lees, S.; Rubio, J.A.; Ruggiero, G.; Ruschmeier, D.; Rykaczewski, H.; Saremi, S.; Sarkar, S.; Salicio, J.; Sanchez, E.; Sanders, M.P.; Sarakinos, M.E.; Schafer, C.; Schegelsky, V.; Schmidt-Kaerst, S.; Schmitz, D.; Schopper, H.; Schotanus, D.J.; Schwering, G.; Sciacca, C.; Sciarrino, D.; Seganti, A.; Servoli, L.; Shevchenko, S.; Shivarov, N.; Shoutko, V.; Shumilov, E.; Shvorob, A.; Siedenburg, T.; Son, D.; Smith, B.; Spillantini, P.; Steuer, M.; Stickland, D.P.; Stone, A.; Stoyanov, B.; Straessner, A.; Sudhakar, K.; Sultanov, G.; Sun, L.Z.; Suter, H.; Swain, J.D.; Szillasi, Z.; Sztaricskai, T.; Tang, X.W.; Tauscher, L.; Taylor, L.; Tellili, B.; Timmermans, Charles; Ting, Samuel C.C.; Ting, S.M.; Tonwar, S.C.; Toth, J.; Tully, C.; Tung, K.L.; Uchida, Y.; Ulbricht, J.; Valente, E.; Vesztergombi, G.; Vetlitsky, I.; Vicinanza, D.; Viertel, G.; Villa, S.; Vivargent, M.; Vlachos, S.; Vodopianov, I.; Vogel, H.; Vogt, H.; Vorobev, I.; Vorobov, A.A.; Vorvolakos, A.; Wadhwa, M.; Wallraff, W.; Wang, M.; Wang, X.L.; Wang, Z.M.; Weber, A.; Weber, M.; Wienemann, P.; Wilkens, H.; Wu, S.X.; Wynhoff, S.; Xia, L.; Xu, Z.Z.; Yamamoto, J.; Yang, B.Z.; Yang, C.G.; Yang, H.J.; Yang, M.; Ye, J.B.; Yeh, S.C.; Zalite, A.; Zalite, Yu.; Zhang, Z.P.; Zhu, G.Y.; Zhu, R.Y.; Zichichi, A.; Zilizi, G.; Zoller, M.

    2000-01-01

    We search for anomalous trilinear gauge couplings in the ${\\rm ZZ}\\gamma$ and ${\\rm Z}\\gamma\\gamma$ vertices using data collected with the L3 detectorat LEP at a centre--of--mass energy $\\sqrt{s}=189$ GeV. No evidence is found and limits on these couplings and on new physics scales are derived from the analysis of the process ${\\rm e}^{+}{\\rm e}^{-} \\rightarrow {\\rm Z} \\gamma$.

  1. The Effects of 23-Gauge Pars Plana Vitrectomy on Orbital Circulation Using Doppler Ultrasonography in Diabetic Macular Edema with Epiretinal Membrane and Taut Posterior Hyaloid.

    Science.gov (United States)

    Gungel, Hulya; Altan, Cigdem; Baylancicek, Deniz Oygar; Karagoz, Yesim; Kisbet, Tanju

    2017-01-01

    To evaluate using Doppler ultrasonography (DUS) how pars plana vitrectomy (PPV) affects orbital circulation in diffuse diabetic macular edema (DME) associated with either the epiretinal membrane (ERM) or taut posterior hyaloid (TPH). The sample included 46 eyes of 42 patients with DME associated with the ERM (n = 22, Group 1) or TPH (n = 24, Group 2). All participants received panretinal laser photocoagulation and antivascular endothelial growth factor injections preoperatively and underwent 23-gauge PPV combined with ERM or TPH removal and internal limiting membrane (ILM) peeling. Pre- and postoperative peak systolic velocity (PSV), end-diastolic velocity (EDV), and the resistivity index (RI) of the ophthalmic artery (OA), central retinal artery (CRA), posterior ciliary artery (PCA), and central retinal vein were measured with DUS. Statistically significant decreases in the PSV and EDV of the OA, CRA, and PCA were detected in all groups. In Group 1, the PSV of the OA and CRA as well as the EDV and PSV of the PCA declined significantly. In Group 2, the EDV of the OA and both the PSV and EDV of the CRA and PCA decreased. Postoperatively, the CRA's PSV and EDV were lower in Group 2, while the preoperative and postoperative RI of the CRA and preoperative RI of the PCA were greater in Group 2 than in Group 1. Changes in the CRA's RI, PSV, and EDV were greater in Group 2 after surgery. 23-Gauge PPV combined with ERM or TPH removal and ILM peeling in DME reduces blood flow rates of both choroidal and retinal vessels. In eyes with TPH, the RIs of the CRA and PCA were significantly greater preoperatively and the changes in the CRA's RI, PSV, and EDV were greater postoperatively. The removal of the TPH may play a role in regulating blood flow.

  2. Multiplicity of carbohydrate-binding sites in β-prism fold lectins ...

    Indian Academy of Sciences (India)

    PRAKASH KUMAR

    have threefold symmetrical subunits, each subunit binds only one sugar. Also, the symmetry in the three-dimensional structure is not reflected in the sequence. ...... Alok Sharma et al. 1104. J. Biosci. 32(6), September 2007. AAT73. 2. 01 .1 [G ym nosp erm]. (3. ) Tax a ce a e. A. A. B. 6. 42. 3. 8 .1. [M on oc o t]. (3. ) Allia c ea e.

  3. Distribution of streptogramin resistance determinants among Enterococcus faecium from a poultry production environment of the USA.

    Science.gov (United States)

    Hayes, Joshua R; Wagner, David D; English, Linda L; Carr, Lewis E; Joseph, Sam W

    2005-01-01

    The impact of agricultural use of antimicrobials on the present and future efficacy of therapeutic drugs in human medicine is a growing public concern. Quinupristin/dalfopristin has been approved to treat human disease caused by vancomycin-resistant Enterococcus faecium and is related to virginiamycin, a streptogramin complex that has long been used in USA agriculture poultry production. Streptogramin-resistant isolates of E. faecium from poultry production environments on the eastern seaboard were recovered without selection for streptogramin resistance and examined using ribotyping to evaluate clonal bias. Colony PCR screening for the previously described streptogramin resistance determinants erm(A), erm(B), msr(C), vgb(A), vat(D) and vat(E) was performed to determine the prevalence of streptogramin resistance mechanisms from these environments. The collection of E. faecium isolates was unevenly distributed among 28 ribogroups and did not cluster geographically. The most prevalent ribogroups was composed of isolates that possessed diverse antimicrobial resistance profiles. Of the 127 isolates examined, 63% were resistant to quinupristin/dalfopristin. The resistance determinants erm(A) and erm(B) were observed among 6% and 10%, respectively, of streptogramin-resistant isolates. msr(C) was detected in a single isolate that was resistant to macrolide and lincosamide antimicrobials. The streptogramin B hydrolase vgb(A) and the streptogramin A acetyltransferases genes vat(D) and vat(E) were not detected in any of the E. faecium isolates. These results indicate that there is widespread resistance to streptogramin antimicrobials among E. faecium throughout the poultry production region in this study and that the mechanisms of resistance to streptogramin antimicrobials within this population remain largely uncharacterized.

  4. Epidemiological study of erythromycin-resistant Streptococcus pyogenes from Korea and Japan by emm genotyping and multilocus sequence typing.

    Science.gov (United States)

    Takahashi, Takashi; Arai, Kazuaki; Lee, Dong Hyun; Koh, Eun Ha; Yoshida, Haruno; Yano, Hisakazu; Kaku, Mitsuo; Kim, Sunjoo

    2016-01-01

    We determined the epidemiological characteristics of erythromycin (EM)-resistant Streptococcus pyogenes (group A streptococci, GAS) strains isolated from Korea and Japan, using emm genotyping and multilocus sequence typing (MLST). Clinical isolates of GAS had been collected from 1992 to 2012 in Korea and from 2004 to 2009 in Japan. EM resistance was determined by the microdilution method, and resistance genotypes were assessed by PCR. The emm genotyping and MLST were performed by DNA sequencing. The emm genotypes and sequence types (STs) were concordant in 143 (85.1%) of 168 EM-resistant GAS strains from Korea. ST36/emm12 (35.1%), ST52/emm28 (22.6%), and ST49/emm75 (16.1%) were the most common types. Most of the ST36 (93.9%) and ST52 (95.8%) strains harbored erm(B), whereas strains ST49, ST42, and ST15 contained mef(A). The concordance between emm genotypes and STs was 41 (93.2%) among 44 EM-resistant GAS strains from Japan. ST36/emm12 (34.1%), ST49/emm75 (18.2%), and ST28/emm1 (15.9%) were the major types. ST36 isolates harbored either erm(B) (56.3%) or mef(A) (37.5%), whereas isolates ST28, ST49, and ST38 carried only mef(A). The proportion of erm(B) and mef(A) was 66.1% and 33.3% in Korea and 22.7% and 68.2% in Japan, respectively. The common STs in Korea and Japan were ST36 and ST49, whereas ST52 was present only in Korea and ST28 only in Japan. Genotype erm(B) was predominant in Korea, whereas mef(A) was frequent in Japan. There were differences between Korea and Japan regarding the frequencies of emm genotypes, STs, and EM resistance genes among the EM-resistant GAS.

  5. Erythromycin resistance features and biofilm formation affected by subinhibitory erythromycin in clinical isolates of Staphylococcus epidermidis.

    Science.gov (United States)

    He, Hong-Jing; Sun, Feng-Jun; Wang, Qian; Liu, Yao; Xiong, Li-Rong; Xia, Pei-Yuan

    2016-02-01

    Subminimal inhibitory concentration (sub-MIC) of antibiotics can modify the phenotype of biofilm formation in bacteria. However, the relationship between resistance phenotypes, genotypes, and the biofilm formation phenotype in response to sub-MIC antibiotics remains unclear. Here, we collected 96 clinical isolates of Staphylococcus epidermidis (S. epidermidis) and investigated the erythromycin (ERY) susceptibility, the biofilm formation in respond to sub-MIC ERY, the presence and transcription expression of erm genes. Serial passage of induction resistance was used against ERY-susceptible isolates and biofilm formation in response to their new sub-MIC ERY was determined. The incidence of biofilm phenotype modification in ERY-resistant isolates was significantly higher than that of ERY-susceptible isolates [27/85 (31.8%) vs. 0/11 (0%), p = 0.031]. Yet, ERY-susceptible isolates displayed the phenomenon of biofilm phenotype modification (7/11), after induction of resistance to ERY. The ermC gene was absolutely dominant among the three macrolide resistant genes including erm (A, B, C) [6/96 (6.2%), 6/96 (6.2%), and 91/96 (94.8%), respectively]. With statistic stratification analysis, a linear and positive correlation was identified between the two factors in the biofilm-enhanced strains, a linear and negative correlation in biofilm-inhibited strains, and a weakly positive correlation in biofilm-unaffected strains (R(2) = 0.4992, 0.3686, and 0.0512, respectively). The results suggest that the ERY resistance phenotype and the transcription expression of ermC gene could be considered as important signs to estimate whether the biofilm formation phenotype in S. epidermidis clinical isolates can be easily affected by sub-MIC ERY. Copyright © 2014. Published by Elsevier B.V.

  6. Genotyping and serotyping of macrolide and multidrug resistant Streptococcus pneumoniae isolated from carrier children

    Directory of Open Access Journals (Sweden)

    S F Swedan

    2016-01-01

    Full Text Available Aims: Streptococcus pneumoniae, an opportunistic pathogen commonly carried asymptomatically in the nasopharynx of children, is associated with increasing rates of treatment failures due to a worldwide increase in drug resistance. We investigated the carriage of S. pneumoniae in children 5 years or younger, the identity of prevalent serotypes, the rates of resistance to macrolides and other antimicrobial agents and the genotypes responsible for macrolide resistance. Materials and Methods: Nasopharyngeal swabs were collected from 157 children under 5 years for cultural isolation of S. pneumoniae. Antibiogram of isolates  was determined using the disk diffusion test, and the minimal inhibitory concentration to macrolides was determined using the E-test. Isolate serotypes and macrolide resistance genes, erm(B and mef(E, were identified using multiplex polymerase chain reactions. Results: S. pneumoniae was recovered from 33.8% of children; 41.9% among males and 21.9% among females (P = 0.009. The highest carriage rate occurred among age groups 7-12 months and 49-60 months. Most frequent serotypes were 19F, 6A/B, 11A, 19A, 14 and 15B/C.  Resistance to macrolides was 60.4%. Resistance to oxacillin, trimethoprim/sulfamethoxazole and clindamycin was present among 90.6%, 54.7% and 32.1% of isolates, respectively. All isolates were susceptible to chloramphenicol, levofloxacin and vancomycin. Isolates resistant to one or more macrolide drugs were more likely to be multidrug resistant. Resistance to clindamycin or oxacillin coexisted with macrolide resistance. Among the erythromycin-resistant isolates, erm(B, mef(E and erm(B and mef(E genes were present at rates of 43.8%, 37.5% and 6.3%, respectively. Erm(B and mef(E were associated with very high level and moderate-to-high level resistance to macrolides, respectively. Conclusion: A significant proportion of children harboured macrolide and multidrug-resistant S. pneumoniae.

  7. [Mechanism of hetero-erythromycin resistant Staphylococcus aureus and a comparison of detection methods].

    Science.gov (United States)

    Chen, Dong-ke; Lai, Hui-ying; Yang, Dong-mei; Xu, Hong-tao

    2013-12-24

    To explore the phenotypes and genotypes of Staphylococcus aureus (S. aureus) hetero-resistant to erythromycin and clindamycin and compare their detection methods so as to report results accurately to guide clinical rational use of antibiotics. D test was used to detect the phenotypes of S. aureus hetero-resistant to erythromycin. And then the results of two methods (automated instrument and disk diffusion) were analyzed. All strains were continuously passaged for 50 generations to verify the phenotypic and genotypic stability of hetero-resistance. ErmA, ermC and msrA genes were amplified by multiplex polymerase chain reaction (PCR). Among 95 erythromycin-sensitive strains, there were 70 strains hetero-resistant to erythromycin (73.7%). The primary 70 strains were all susceptive to erythromycin (MIC ≤ 0.25 µg/ml) and clindamycin (MIC ≤ 0.25 µg/ml) with the cards of GP-67 of VITEK2 Compact. With D tests, the results were difficult to observe. The passaged 70 strains were all resistant to erythromycin (MIC >8 µg/ml) and susceptible to clindamycin (MIC ≤ 0.25 µg/ml) and D test positive with the cards of GP-67 of VITEK2 Compact. The primary and 50(th) generation of herero-erythromycin resistant strains were stable in susceptibility test results. The primary and the 50(th)th generation strains were all ermA gene positive, ermC and msrA negative with PCR results. The phenotypes and genotypes of hetreo-erythromycin resistant S. aureus strains were stable. Missed detection with VITEK2 Compact may affect the proper use of erythromycin and clindamycin. Laboratory technicians should identify the erythromycin-susceptible strains by disk diffusion method.

  8. Aufklärung des Europäischen Forschungsraums durch Geschlechterwissen?

    Directory of Open Access Journals (Sweden)

    Heike Kahlert

    2008-07-01

    Full Text Available Zentrales Verdienst der vorliegenden empirischen Studie ist es, anschaulich das Netzwerk von Wissenschaftlerinnen, Politikerinnen sowie Mitarbeiterinnen und Mitarbeitern der nationalen und insbesondere der europäischen Forschungsverwaltung zu beschreiben, das ermöglichte, Gender Mainstreaming im Sechsten Forschungsrahmenprogramm der EU zu implementieren. Außerdem wird die Verknüpfung aus formellen und informellen Politikstrukturen und Genderexpertise beschrieben.

  9. Villa Miidurannas = Villa in Miiduranna / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    2004-01-01

    Maja on kolmest küljest suletud kast, lahtine on vaid merepoolne külg, pälvis 2003. a. parima betoonehitise preemia. Projekteerija: AB Emil Urbel. Autor Emil Urbel, kaasa töötas Indrek Erm. Sisekujundus: Katrin ja Argo Vaikla, Tiina Teng (Vaikla Disain). Konstruktor TA Konsult. Projekt 2001, valmis 2004. Üldpind: 528 mø. Ill.: I, II ja III korruse plaan, 3 välis- ja 5 sisevaadet, värv

  10. Filmiõhtu võitis tibikomöödia / Merili Nikkolo

    Index Scriptorium Estoniae

    Nikkolo, Merili

    2008-01-01

    Paide gümnaasiumi filmiõhtu, kus kultuurikeskuses näidati õpilaste valmistatud filme, valis žürii ( Urmas-Eero Liiv, Antti Oolo, Olaf Suuder) võidufilmiks "Kuidas ma talle ütlen, et olen rase". Filmi režissöör ja peaosaline on 9.klassi õpilane Margret Järv, abi osutas noorte MTÜ M&B Studio juht Bionka Erm

  11. Molecular characterization of invasive Streptococcus dysgalactiae subsp. equisimilis. Multicenter study: Argentina 2011-2012.

    Science.gov (United States)

    Traverso, Fernando; Blanco, Alejandra; Villalón, Pilar; Beratz, Noelia; Sáez Nieto, Juan Antonio; Lopardo, Horacio

    Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of the isolates were recovered from blood (60.9%). All isolates carried speJ and ssa genes. stG62647, stG653 and stG840 were the most frequent emm types. Nineteen different PFGE patterns were detected. All isolates were susceptible to penicillin and levofloxacin, 6 (26.1%) showed resistance or reduced susceptibility to erythromycin [1 mef(A), 3 erm(TR), 1 mef(A)+erm(TR) and 1 erm(TR)+erm(B)] and 7 (30.4%) were resistant or exhibited reduced susceptibility to tetracycline [2 tet(M), 5 tet(M)+tet(O)]. The prevalence in Argentina was of at least 23 invasive infections by SDSE. A wide genetic diversity was observed. All isolates carried speJ and ssa genes. Similarly to other studies, macrolide resistance (26.1%) was mainly associated to the MLS B phenotype. Copyright © 2016 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  12. Die Radiofrequenz-Kyphoplastie zur Behandlung osteoporotischer und neoplastischer Wirbelkörperfrakturen. Erste Erfahrungen und klinische Ergebnisse nach 6 Monaten

    OpenAIRE

    Elgeti F; Gebauer B

    2010-01-01

    Die Vertebroplastie und die konventionelle Kyphoplastie mittels Ballon oder Stent sind etablierte Verfahren zur Behandlung schmerzhafter, konservativ-medikamentös therapieresistenter, osteoporotischer und neoplastischer Wirbelkörperfrakturen. Die Radiofrequenz-Kyphoplastie mithilfe eines ultrahochviskösen Zements stellt eine innovative Methode dar. Sie ermöglicht eine sichere und effektive Schmerztherapie bei osteoporotischen und neoplastischen Wirbelkörperfrakturen bei gleichzeitig...

  13. Die Radiofrequenz-Kyphoplastie zur Behandlung osteoporotischer und neoplastischer Wirbelkörperfrakturen. Erste Erfahrungen und klinische Ergebnisse nach 6 Monaten

    Directory of Open Access Journals (Sweden)

    Elgeti F

    2010-01-01

    Full Text Available Die Vertebroplastie und die konventionelle Kyphoplastie mittels Ballon oder Stent sind etablierte Verfahren zur Behandlung schmerzhafter, konservativ-medikamentös therapieresistenter, osteoporotischer und neoplastischer Wirbelkörperfrakturen. Die Radiofrequenz-Kyphoplastie mithilfe eines ultrahochviskösen Zements stellt eine innovative Methode dar. Sie ermöglicht eine sichere und effektive Schmerztherapie bei osteoporotischen und neoplastischen Wirbelkörperfrakturen bei gleichzeitiger Schonung der intakten trabekulären Knochensubstanz.

  14. Möglichkeiten der direkten Zementinjektionstechnik bei Wirbelkörperfrakturen - Drei Fallbeschreibungen der Radiofrequenz-Kyphoplastie (RFK mit ultrahochvikösem Knochenzement

    Directory of Open Access Journals (Sweden)

    Jonas M

    2012-01-01

    Full Text Available Konventionelle Kyphoplastie mittels Ballon oder Stent ist ein etabliertes Verfahren zur Behandlung schmerzhafter, konservativ-medikamentös therapieresistenter, osteoporotischer und neoplastischer Wirbelkörperfrakturen. Wir berichten über drei Patienten, die mittels der innovativen Radiofrequenz-Kyphoplastie mit ultrahochviskösem Zement therapiert wurden. Dieses Verfahren ermöglicht eine effektive und sichere Schmerztherapie bei Wirbelkörperfrakturen unter gleichzeitiger Schonung der intakten trabekulären Knochensubstanz.

  15. Entlang der Grenzen zwischen Nähe und Distanz: Beziehungsgestaltung in der Sozialberatung

    OpenAIRE

    Indermühle, Annina; Zürcher-Hauck, Bernadette

    2014-01-01

    Die Frage nach einer angepassten und ausgewogenen Beratungsbeziehung beschäftigt Berufs-einsteigende ebenso wie erfahrene Fachleute der Sozialarbeit. Die vorliegende Literaturarbeit setzt sich mit Wirkgefügen von Einflussfaktoren und Handlungsansätzen auseinander. Ziel ist es, entlang der Grenzen von Nähe und Distanz praxisorientierte Erkenntnisse und Methoden aufzu¬zeigen, die eine adäquate Beziehungsgestaltung in der Sozialberatung ermöglichen. Nach der Theorie von Hans Thiersch orientiert ...

  16. Enterprise Risk Management: The Way Ahead for DRDC within the DND Enterprise

    Science.gov (United States)

    2010-03-01

    Management – Integrated Framework , COSO Executive Summary, Sept 2004 2. Institute of Risk Management (IRM) Risk Management Standard, 2002 3...consistently at all levels because a framework of the type envisaged in ISO 31000 has not been implemented. IRM is thus far ad hoc in its...enterprise risk management framework and process. Second, the most crucial element of implementing ERM, the organizational culture, is discussed. Third

  17. Einfluss des Mammographie-Screenings in der Prävalenzphase auf Tumorstaging und Operationsverfahren bei Brustkrebspatientinnen

    OpenAIRE

    Müller geb. Doll, Luisa

    2015-01-01

    Das Mammakarzinom stellt als die häufigste Krebserkrankung der Frau eine besondere medizinische Herausforderung dar. Denn nur eine frühe Diagnose, die mit einem lokal begrenzten Tumor und günstigen Prognosefaktoren assoziiert ist, ermöglicht in vielen Fällen kurative Therapieansätze. So ist neben der stetigen Verbesserung der Therapie vor allem die Früherkennung von entscheidender Bedeutung für die Prognose. Das Mammograp...

  18. Die häufigsten Fehler bei der FMEA-Anwendung und wie ich sie vermeiden kann

    OpenAIRE

    Schloske, Alexander

    2010-01-01

    Die FMEA stellt heutzutage eines der wichtigsten Werkzeuge des präventiven Risikomanagements dar. Die universell anwendbare Vorgehensweise des VDA hat sich dabei in der Industrie etabliert. Der Beitrag beschreibt aus der Sicht von 20 Jahren FMEA-Erfahrung, wie die FMEA zielführend erstellt wird und mit welchen Tipps und Tricks eine effiziente und effektive FMEA-Erstellung ermöglicht wird.

  19. Vorgehensweisen innerhalb der FMEA-Arten

    OpenAIRE

    Schloske, Alexander

    2014-01-01

    Die FMEA stellt heutzutage eines der wichtigsten Werkzeuge des präventiven Risikomanagements dar. Die universell anwendbare Vorgehensweise des VDA hat sich dabei in der Industrie etabliert. Der Beitrag beschreibt aus der Sicht von 25 Jahren FMEA-Erfahrung, wie die FMEA zielführend erstellt wird und mit welchen Tipps und Tricks eine effiziente und effektive FMEA-Erstellung ermöglicht wird. Es wird dabei detailliert dargestellt, wie innerhalb der einzelnen FMEA-Arten (System, Konstruktion, Proz...

  20. Vorgehensweisen innerhalb der FMEA-Arten

    OpenAIRE

    Schloske, Alexander; Mannuß, Oliver

    2015-01-01

    Die FMEA stellt heutzutage eines der wichtigsten Werkzeuge des präventiven Risikomanagements dar. Die universell anwendbare Vorgehensweise des VDA hat sich dabei in der Industrie etabliert. Der Beitrag beschreibt aus der Sicht von 25 Jahren FMEA-Erfahrung, wie die FMEA zielführend erstellt wird und mit welchen Tipps und Tricks eine effiziente und effektive FMEA-Erstellung ermöglicht wird. Es wird dabei detailliert dargestellt, wie innerhalb der einzelnen FMEA-Arten (System, Konstruktion, Proz...

  1. Oxidative transformation of micropollutants during municipal wastewater treatment: comparison of kinetic aspects of selective (chlorine, chlorine dioxide, ferrate VI, and ozone) and non-selective oxidants (hydroxyl radical).

    Science.gov (United States)

    Lee, Yunho; von Gunten, Urs

    2010-01-01

    Chemical oxidation processes have been widely applied to water treatment and may serve as a tool to minimize the release of micropollutants (e.g. pharmaceuticals and endocrine disruptors) from municipal wastewater effluents into the aquatic environment. The potential of several oxidants for the transformation of selected micropollutants such as atenolol, carbamazepine, 17 alpha-ethinylestradiol (EE2), ibuprofen, and sulfamethoxazole was assessed and compared. The oxidants include chlorine, chlorine dioxide, ferrate(VI), and ozone as selective oxidants versus hydroxyl radicals as non-selective oxidant. Second-order rate constants (k) for the reaction of each oxidant show that the selective oxidants react only with some electron-rich organic moieties (ERMs), such as phenols, anilines, olefins, and deprotonated-amines. In contrast, hydroxyl radicals show a nearly diffusion-controlled reactivity with almost all organic moieties (k>or=10(9)M(-1) s(-1)). Due to a competition for oxidants between a target micropollutant and wastewater matrix (i.e. effluent organic matter, EfOM), a higher reaction rate with a target micropollutant does not necessarily translate into more efficient transformation. For example, transformation efficiencies of EE2, a phenolic micropollutant, in a selected wastewater effluent at pH 8 varied only within a factor of 7 among the selective oxidants, even though the corresponding k for the reaction of each selective oxidant with EE2 varied over four orders of magnitude. In addition, for the selective oxidants, the competition disappears rapidly after the ERMs present in EfOM are consumed. In contrast, for hydroxyl radicals, the competition remains practically the same during the entire oxidation. Therefore, for a given oxidant dose, the selective oxidants were more efficient than hydroxyl radicals for transforming ERMs-containing micropollutants, while hydroxyl radicals are capable of transforming micropollutants even without ERMs. Besides Ef

  2. Activities of a New Fluoroketolide, HMR 3787, and Its (Des)-Fluor Derivative RU 64399 Compared to Those of Telithromycin, Erythromycin A, Azithromycin, Clarithromycin, and Clindamycin against Macrolide-Susceptible or -Resistant Streptococcus pneumoniae and S. pyogenes

    Science.gov (United States)

    Nagai, Kensuke; Davies, Todd A.; Ednie, Lois M.; Bryskier, Andre; Palavecino, Elizabeth; Jacobs, Michael R.; Appelbaum, Peter C.

    2001-01-01

    Activities of HMR 3787 and RU 64399 were compared to those of three macrolides, telithromycin, and clindamycin against 175 Streptococcus pneumoniae isolates and 121 Streptococcus pyogenes isolates. HMR3787 and telithromycin were the most active compounds tested against pneumococci. Telithromycin and RU 64399 were equally active against macrolide-susceptible (MICs, 0.008 to 0.06 μg/ml) and -resistant S. pyogenes isolates, but HMR 3787 had lower MICs for ermB strains. PMID:11600391

  3. Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida

    OpenAIRE

    Rose, Simon; Desmolaize, Benoit; Jaju, Puneet; Wilhelm, Cornelia; Warrass, Ralf; Douthwaite, Stephen

    2012-01-01

    The bacterial pathogens Mannheimia haemolytica and Pasteurella multocida are major etiological agents in respiratory tract infections of cattle. Although these infections can generally be successfully treated with veterinary macrolide antibiotics, a few recent isolates have shown resistance to these drugs. Macrolide resistance in members of the family Pasteurellaceae is conferred by combinations of at least three genes: erm(42), which encodes a monomethyltransferase and confers a type I MLSB ...

  4. Identification of Cytoplasmic Proteins Interacting with the Mammary Cell Transforming Domain of Ese-1

    Science.gov (United States)

    2009-04-01

    gynecological tumors. In cervical cancer, over- expression of ETS1, ERM (also called ETV5), ERG or ETS2 was associated with higher grade, metastasis and...and metastasis in the development and progression of gynecological malignancies. Increases in ETS1 and ELF1 are also linked to the malignant potential...tended to contain regions with cytologic features of high-grade dysplasia, including large nuclei with prominent nucleoli and brisk mitotic activity

  5. Experiment list: SRX1092614 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available s musculus; ChIP-Seq source_name=Short-term Hematopoietic stem cells || strain=C57BL/6J || material source=b...one marrow from 8-week old C57BL/6J mice || sorting=lin-, Sca1+, c-Kit+, Flk2-, CD34+ || cell type=Short-t...erm Hematopoietic stem cells || chip antibody=anti-H3K4me3 (Cell Signaling, catalog

  6. Experiment list: SRX1092613 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available s musculus; ChIP-Seq source_name=Short-term Hematopoietic stem cells || strain=C57BL/6J || material source=b...one marrow from 8-week old C57BL/6J mice || sorting=lin-, Sca1+, c-Kit+, Flk2-, CD34+ || cell type=Short-t...erm Hematopoietic stem cells || chip antibody=anti-H3K4me3 (Cell Signaling, catalog

  7. Neuentwicklung eines elektronischen Antriebes für ein Koloskop - Ermittlung grundlegender Anforderungen und Messung von Vorschubkräften

    OpenAIRE

    Beron, Katja

    2012-01-01

    Hintergrund und Ziele: Die Koloskopie als ein etablierter Bestandteil des Screenings und der Früherkennung des Kolorektalen Karzinoms gewinnt durch technische Weiterentwicklungen gerade bei interventionellen Eingriffen zunehmend an Bedeutung. Zur Verbesserung der Untersuchung soll eine mechanische Lösung entwickelt werden, die die Bewegung des Koloskops durch den Arzt allein ermöglicht und durch die direkte Steuerung und die Limitierung der anwendbaren Kraft mehr Sicherheit bietet. Das führte...

  8. Entwicklung eines Konzepts zum Lagermanagement mit Optimierungsansätzen auf Ebene eines neuen Regalbediengerätes

    OpenAIRE

    Salah, Bashir; Strehle, Marc; Noche, Bernd

    2012-01-01

    Die Grundidee hinter dem neuen Regalbediengerät ist die Minimierung der zu bewegenden Last durch die Verwendung einer seilbasierten Stewart-Gough-Plattform (SGP). Das klassische Regalbediengerät (RBG) wird dabei durch eine Plattform ersetzt, die an bis zu acht vorgespannten Seilen befestigt ist. Unterstützt wird diese Bauform durch eine abgestimmte Steuerungssoftware, die es ermöglicht die Vorteile des RBG optimal zu nutzen. Das hier mit Software vorgestellte Konzept regelt die Vorgehensweise...

  9. Antimicrobial Drug Use and Macrolide-Resistant Streptococcus pyogenes, Belgium

    OpenAIRE

    Van Heirstraeten, Liesbet; Coenen, Samuel; Lammens, Christine; Hens, Niel; Goossens, Herman; Malhotra-Kumar, Surbhi

    2012-01-01

    In Belgium, decreasing macrolide, lincosamide, streptogramins B, and tetracycline use during 1997-2007 correlated significantly with decreasing macrolide-resistant Streptococcus pyogenes during 1999-2009. Maintaining drug use below a critical threshold corresponded with low-level macrolide-resistant S. pyogenes and an increased number of erm(A)-harboring emm77 S. pyogenes with low fitness costs. This study was partly funded by the Belgian Antibiotic Policy Coordination Committee. Immuno...

  10. Erlebbarkeit von Anlagenkomponenten im Kontext Virtuelle Inbetriebnahme in virtuellen Umgebungen

    OpenAIRE

    Geiger, Andreas; Rehfeld, Ingolf; Rothenburg, Uwe; Stark, Rainer

    2016-01-01

    Aus der Einleitung "Der Einsatz von Virtual Reality (VR) Methoden in der Fabrikplanung und Absicherung ist bei großen produzierenden Unternehmen heute in allen Phasen des Produktentwicklungsprozesses (PEP) „State of the Art“ (Runde 2012). Virtual Reality ermöglicht die frühzeitige Visualisierung eines Entwicklungsstands in Originalgröße. Dadurch lassen sich Design- oder Konzeptentwürfe visualisieren, frühzeitig Fehler erkennen und Absicherungen hinsichtlich Ergonomie, oder Ein- und Ausbauu...

  11. Risk Management and Risk Management Failure: Lessons for Business Enterprises

    OpenAIRE

    Olajide Solomon Fadun

    2013-01-01

    The recent economic volatility gives risk management a new focus and eminence. Successful firms are able and willing to effectively integrate risk management at all levels of management process. The purpose of the study is to highlight the importance of effective risk management (ERM) in preventing risk management failure. Risk management failure prevents firms’ from meeting their expectations; thus, results to repeated business and project failures. Although the degree of risk management act...

  12. Ein semi-analytisches Modell zur dynamischen Berechnung des gekoppelten Systems Fahrzeug-Fahrweg-Untergrund für das Oberbausystem Feste Fahrbahn

    OpenAIRE

    Dinkel, Jens

    2007-01-01

    Zur dynamischen Berechnung des Oberbausystems Feste Fahrbahn wird ein semi-analytisches Modell entwickelt, das die wesentlichen Elemente des Schienenverkehrs enthält. Das Fahrzeug, der Oberbau und der Untergrund sind durch geeignete mechanische Modelle abgebildet und über dynamische Interaktionsbeziehungen verknüpft. Als Lösungsverfahren kommt die Fouriertransformation zum Einsatz, die eine Betrachtung und Bewertung der dynamischen Effekte im Frequenz- und Wellenzahlraum ermöglicht. Die Tragp...

  13. Evaluation of experimental field data from the modified earth resistance method used in trees and stands as integrated with selected classically estimated soil properties

    OpenAIRE

    Jagoš, Pavel

    2016-01-01

    This experiment tested the possibility of adding other information about the soil physical properties to existing modified earth impedance method, published by Čermák, Staněk, Aubrecht in 2006. For this experiment, this method for resistivity measurement of tree (trunk/soil), was employed. The measurements were conducted on four different study plots with different soil textural classes located in the Czech Republic. The choice of localities was guided the most typical soil textural classes; ...

  14. Selbstbildung und Gewalt in jugendlichen Cliquen

    OpenAIRE

    Wetzstein, Thomas; Erbeldinger, Patricia; Hilgers, Judith; Eckert, Roland

    2003-01-01

    Freizeitcliquen von Jugendlichen können als Orte von Selbstbildung i.S. eines selbstgesteuerten Lernens begriffen werden, indem Erfahrungen gemeinsam interpretiert und in Handlungen umgesetzt werden. Jugendliche entsprechen damit dem diffusen Auftrag, etwas "aus sich zu machen", den sie von ihren Eltern und den Erziehungsinstitutionen erhalten haben. [In dem Beitrag] werden zwei Typen von Cliquenzugehörigkeit herausgegriffen: "Prekäre Cliquen" ermöglichen ihren Mitgliedern nur eingeschränkt "...

  15. Romania’s Dilemmas in Perspective of EMU Integration

    Directory of Open Access Journals (Sweden)

    Aura Niculescu

    2006-04-01

    Full Text Available Our study evaluates the trade-off between real and nominal convergence in transition countries relating European integration (case of Romania. The potential conflict between Maastricht criteria and the possibility to obtain a faster real convergence with the European Union countries is explained with Ballassa-Samuelson effect. In addition, according this effect, we study the monetary policy regimes for Romanian authorities in perspective of the future admittance in Exchange Rate Mechanism II (ERM II

  16. Analyse eines membranunterstützten Reaktivrektifikationsverfahrens zur Umesterung von Methylacetat mit Butanol

    OpenAIRE

    Bolivar, Wilda

    2008-01-01

    Die Kopplung von Reaktivrektifikation und Membrantrennung zu einem reaktiven hybriden Trennverfahren stellt einen weiteren Schritt auf dem Weg zu einem intensivierten Produktionsprozess in der chemischen Industrie dar. Bisherige Arbeiten auf diesem Gebiet beschränken sich bislang auf eine Betrachtung einzelner Teilaspekte des kombinierten Prozesses oder basieren auf Modellierungsansätzen, die keine Übertragung auf den industriellen Maßstab ermöglichen. In der vorliegenden Arbeit wird daher da...

  17. Effects of Copper Addition on Copper Resistance, Antibiotic Resistance Genes, and intl1 during Swine Manure Composting

    Science.gov (United States)

    Yin, Yanan; Gu, Jie; Wang, Xiaojuan; Song, Wen; Zhang, Kaiyu; Sun, Wei; Zhang, Xin; Zhang, Yajun; Li, Haichao

    2017-01-01

    Copper is one of the most abundant heavy metals present in swine manure. In this study, a laboratory-scale aerobic composting system was amended with Cu at three levels (0, 200, and 2000 mg kg-1, i.e., control, Cu200, and Cu2000 treatments, respectively) to determine its effect on the fate of copper resistance genes [copper resistance genes (CRGs): pcoA, cusA, copA, and tcrB], antibiotic resistance genes [antibiotic resistance genes (ARGs): erm(A) and erm(B)], and intl1. The results showed that the absolute abundances of pcoA, tcrB, erm(A), erm(B), and intl1 were reduced, whereas those of copA and cusA increased after swine manure composting. Redundancy analysis showed that temperature significantly affected the variations in CRGs, ARGs, and intl1. The decreases in CRGs, ARGs, and intI1 were positively correlated with the exchangeable Cu levels. The bacterial community could be grouped according to the composting time under different treatments, where the high concentration of copper had a more persistent effect on the bacterial community. Network analysis determined that the co-occurrence of CRGs, ARGs, and intI1, and the bacterial community were the main contributors to the changes in CRGs, ARG, and intl1. Thus, temperature, copper, and changes in the bacterial community composition had important effects on the variations in CRGs, ARGs, and intl1 during manure composting in the presence of added copper. PMID:28316595

  18. Die Analyse von genomweiten Re-Sequenzierungsdaten zur Identifizierung kausaler DNA-Variation beim Rind

    OpenAIRE

    Kipp, Sandra

    2015-01-01

    Ziel dieser Arbeit war die Genom-Re-Sequenzierung von Gründertieren verschiedener Rinderpopulationen und die damit verbundene zuverlässige Aufdeckung der genomischen Variation. Die funktionelle Annotation und populationsweite Imputation sequenzbasierter Varianten, sowie deren Anwendung in genomweiten Assoziationsstudien ermöglichten die Identifizierung potentiell kausaler Kandidaten für genetisch bedingte Defekte und Merkmale. Mit Hilfe dieser Informationen kann eine optimierte Anpaarung der ...

  19. Norms and international standards related to reduce risk management: A literature review

    Directory of Open Access Journals (Sweden)

    César Fuentes

    2011-09-01

    Full Text Available The current work aims to develop a revision of the literature within the main concepts in the international rules and standards related to risk management in companies. By this way, there will be an analysis of issues such as the COSO - ERM model, an introduction to the ISO 27000 and 31000 standards; and the Project Management according to PMI targeted at risk management

  20. Alimentaci??n Kosher y certificaci??n Kashrut en el mercado alimenticio internacional: seguridad alimentaria y controles de calidad

    OpenAIRE

    Garc??a Ruiz, Carmen Yolanda

    2012-01-01

    El ??rea de Derecho Eclesi??stico del Estado, junto al Instituto Polit??cnico de Bragan??a (Portugal), en el marco del Proyecto ???Campus de Excelencia Internacional de la Universidad de Le??n CEI ??? ERMES??? organiza un Congreso Internacional sobre ???Alimentaci??n, creencias y diversidad cultural??? en el Sal??n de Grados de la Facultad de Derecho del 8 al 10 de octubre de 2012

  1. Naval Aircraft Operating and Support Cost-Estimating Model - FY77 Revision

    Science.gov (United States)

    1979-02-01

    3 ^PflLi- XTC ^S) DHTH NH.--1£.-- PMT = 1.66975 + .013009+PMD DEP = (-6.U3360 + £.6771+SQPT<:: (DDP+CSE+DCfl+PMT+ECfl)+NH:;’> .•■•HH TDD...nP» FCJ TDJ TBF JRVD J XT ICS) 3 ? PMT? DEP? TDD^ TDE^ PDF-PEN-PDL.i nOCitCRj PRJ EROsr ERM? ERTJ PS 3 JPRLJ XTC ɝ> XTR = flNS flNS

  2. Thrombolyse bei Lungenembolie - Status 2004

    OpenAIRE

    Hief C; Geppert A; Haoula D; Huber K; Koch J

    2004-01-01

    Bei der Lungenembolie handelt es sich um ein häufiges Krankheitsbild, welches durch rasche und effektive Diagnostik risikostratifiziert werden kann und damit eine entsprechend angepaßte Therapie ermöglicht. Von einer Thrombosetherapie profitieren praktisch nur Patienten mit massiver Lungenembolie oder mit nachgewiesener akuter Rechtsherzbelastung, wobei das Echokardiogramm und Troponin-Bestimmungen zur Erkennung hilfreich sind. Neben dem "Goldstandard" rt-PA (100 mg über 2 Stunden) ist in Ö...

  3. A quantitative analysis of e-commerce

    OpenAIRE

    Teltzrow, Maximilian

    2005-01-01

    Die Rolle und Wahrnehmung des World Wide Web in seinen unterschiedlichen Nutzungskontexten ändert sich zunehmend – von einem frühen Fokus auf reine Web-Interaktion mit Kunden, Informationssuchern und anderen Nutzern hin zum Web als eine Komponente in einer mehrkanaligen Informations- und Kommunikationsstrategie. Diese zentrale Entwicklung ermöglicht Firmen, eine wachsende Menge digitaler Konsumenteninformationen zu sammeln, zu analysieren und zu verwerten. Während Firmen von diesen Daten pro...

  4. Entwicklung und Charakterisierung einer photoschaltbaren Histondeacetylase-ähnlichen Amidohydrolase

    OpenAIRE

    Korbus, Michael

    2015-01-01

    Die Kontrolle von Enzymen durch einen externen Lichtstimulus ermöglicht eine präzise, orts- und zeitaufgelöste Steuerung definierter chemischer Reaktionen und demnach die Katalyse bestimmter Substrate. Die vorliegende Arbeit beschreibt die Entwicklung und Charakterisierung eines reversibel photoschaltbaren Biokatalysators, einer Histondeacetylase-ähnlichen Amidohydrolase (HDAH) von Bordetella/Alcaligenes Stamm FB188, welcher in einen aktiveren oder inaktiveren Zustand durch cis/trans Photoiso...

  5. Development of Macro Level Indicators of Restructuring and Workers' Health

    OpenAIRE

    Theorell, Töres; Theodossiou, Ioannis; Mihályi, Péter; Laurijssen, Eddy; Edenharter, Günter; Drakopoulos, Stavros; Andreeva, Elena; Brenner, M. Harvey; Triomphe, Claude Emmanuel

    2011-01-01

    Hauptgegenstand dieses Projektes war es, eine internationale Datenbank (insb. für Europa) von Faktoren herzustellen, die für die gesundheitlichen Auswirkungen von wirtschaftlicher Umstrukturierung und Personalabbau relevant sind. Daten von der WHO, EUROSTAT, OECD, FAO, ILO, Weltbank und der UNO wurden so zusammengeführt, dass sie die Analyse ökonomischer Faktoren, die Umstrukturierungen hervorbringen und die Gesundheit der Bevölkerungen industrialisierter Länder beeinflussen, ermöglichen. Es ...

  6. In-situ tomographic investigation of creep cavity evolution in brass

    OpenAIRE

    Cerceau Isaac Neta Pinto, Augusta

    2009-01-01

    In der Arbeit wurde die Möglichkeit zur zerstörungsfreien Prüfung mittels der Röntgenmikrotomographie genutzt, um in-situ Untersuchungen an Kriechbruch verursachende Hohlraumbildung in Messing zu ermöglichen. An aufeinander folgende rekonstruierte Probenvolumina konnte durch Anwendung von Bildverarbeitungsmethoden die Entwicklung der kriechbedingten Hohlräume verfolgt werden. Die Wachstumsrate von über 1000 einzelnen Hohlräumen konnte im Bereich zwischen der Nachweisgrenze und der...

  7. Reflections on osteopathic fascia treatment in the peripheral nervous system

    OpenAIRE

    Bordoni B; Bordoni G

    2015-01-01

    Bruno Bordoni,1–3 Giovanni Bordoni4 1Department of Cardiology, Santa Maria Nascente Institute IRCCS – Hospitalization and Care with Scientific Address, Don Carlo Gnocchi Foundation, 2School TCIO Milano – Osteopathy Institute, 3Edi-Ermes, Milan, 4CRESO School, Osteopathic Centre for Research and Studies, Falconara Marittima, Ancona, Italy Abstract: The peripheral nerve is composed of several layers of fascia tissue, which can become a source of p...

  8. Umsetzung der Unternehmensstrategie mit der Balanced Scorecard

    Science.gov (United States)

    Crespo, Isabel; Bergmann, Lars; Portmann, Stefan; Lacker, Thomas; Lacker, Michael; Fleischmann, Jürgen; Kozó, Hans

    Die Balanced Scorecard (BSC) ist ein Ansatz zum strategischen Management, der neben der Ausrichtung des Unternehmens auf finanzielle Zielwerte ebenso großes Gewicht auf so genannte weiche Faktoren legt, die den wirtschaftlichen Erfolg eines Unternehmens erst ermöglichen. Das entscheidende Merkmal der Balanced Scorecard ist dabei, dass sie ein ausgewogenes System strategischer Ziele herstellt, welches das Unternehmen hinsichtlich der vier Perspektiven Finanzen, Kunden, interne Prozesse und Mitarbeiter und Potenziale strategisch ausrichtet (Kaplan u. Norton 1997).

  9. Digitalisierung, Visualisierung, Virtualisierung: Von der virtuellen Welt in die reale Produktion, Entwicklung eines Arbeitsplatzes der Zukunft ; Laufzeit des Vorhabens: von: 01.03.2000 bis: 31.10.2000. BMBF-Programm "Produktion 2000". Schlußbericht. Förderkennzeichen 20PV4901

    OpenAIRE

    Klumpp, B.

    2001-01-01

    Innerhalb der an das Leitprojekt "Integrierte Virtuelle Produktentstehung IVIP" angehängten Aktivität "Digitalisierung, Visualisierung, Virtualisierung" sollte ein Pilot aufgebaut werden, der die Produktentstehung und -optimierung prozeßseitig mit Ankoppelung der realen Maschine ermöglicht. Hintergrund ist die schnelle Übertragung der Modelle im Rechner auf die Maschine und die Datenaquisition aus der realen Steuerung. Ein bidirektionaler, echtzeitfähiger Datenfluß zwischen Maschine und Arbei...

  10. A pilot study of morphometric analysis of choroidal vasculature in vivo, using en face optical coherence tomography.

    Directory of Open Access Journals (Sweden)

    Mahsa Sohrab

    Full Text Available To study the ability of volumetric spectral domain optical coherence tomography (SD-OCT to perform quantitative measurement of the choroidal vasculature in vivo.Choroidal vascular density and vessel size were quantified using en face choroidal scans from various depths below the retinal pigment epithelium (RPE in 58 eyes of 58 patients with either epiretinal membranes (ERM, early age-related macular degeneration (AMD, or reticular pseudo-drusen (RPD. For each patient, we used the macular volume scan (6×6 mm cube for vessel quantification, while high-definition (HD cross-section raster scans were used to qualitatively assess vascularity of the choroidal sub-layers, and measure choroidal thickness.Of the 58 patients, more were female (66% versus 34% male, of whom 14 (24% had ERM, 11 (19% early AMD, and 33 (57% RPD. Compared to intact choriocapillaris in all ERM (100%, none of the RPD and only 5/11 (45% early AMD eyes had visible choriocapillaris on either cross section or C-scans (p-value<0.001. When comparing select regions from the most superficial C-scans, early AMD group had lowest vascular density and RPD had highest (p-value 0.04. Qualitative evaluation of C-scans from all three groups revealed a more granular appearance of the choriocapillaris in ERM versus increased stroma and larger vessels in the RPD eyes.SD-OCT can be used to qualitatively and quantitatively assess choroidal vascularity in vivo. Our findings correlate to previously reported histopathologic studies. Lack of choriocapillaris on HD cross-sections or C-scans in all RPD and about half of early AMD eyes suggests earlier choroidal involvement in AMD and specifically, RPD.

  11. Antimicrobial resistance genes in Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida isolated from Australian pigs.

    Science.gov (United States)

    Dayao, Dae; Gibson, J S; Blackall, P J; Turni, C

    2016-07-01

    To identify genes associated with the observed antimicrobial resistance in Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida isolated from Australian pigs. Isolates with known phenotypic resistance to β-lactams, macrolides and tetracycline were screened for the presence of antimicrobial resistance genes. A total of 68 A. pleuropneumoniae, 62 H. parasuis and 20 P. multocida isolates exhibiting phenotypic antimicrobial resistance (A. pleuropneumoniae and P. multocida) or elevated minimal inhibitory concentrations (MICs) (H. parasuis) to any of the following antimicrobial agents - ampicillin, erythromycin, penicillin, tetracycline, tilmicosin and tulathromycin - were screened for a total of 19 associated antimicrobial resistance genes (ARGs) by PCR. The gene bla ROB-1 was found in all ampicillin- and penicillin-resistant isolates, but none harboured the bla TEM-1 gene. The tetB gene was found in 76% (74/97) of tetracycline-resistant isolates, 49/53 A. pleuropneumoniae, 17/30 H. parasuis and 8/14 P. multocida. One A. pleuropneumoniae isolate harboured the tetH gene, but none of the 97 isolates had tetA, tetC, tetD, tetE, tetL, tetM or tetO. A total of 92 isolates were screened for the presence of macrolide resistance genes. None was found to have ermA, ermB, ermC, erm42, mphE, mefA, msrA or msrE. The current study has provided a genetic explanation for the resistance or elevated MIC of the majority of isolates of Australian porcine respiratory pathogens to ampicillin, penicillin and tetracycline. However, the macrolide resistance observed by phenotypic testing remains genetically unexplained and further studies are required. © 2016 Australian Veterinary Association.

  12. Katheterablation von Vorhofflimmern: Pulmonalvenen-Anatomie und interventionelle Implikation

    OpenAIRE

    Lickfett L; Lewalter T; Nickenig G

    2007-01-01

    Die Ablationstherapie der Pulmonalvenen (PV) hat nicht nur die Behandlungsoptionen von Vorhofflimmern, sondern auch das pathophysiologische Verständnis dieser überaus wichtigen Herzrhythmusstörung wesentlich erweitert. Die morphologische Erforschung der PV in vivo wurde erst durch moderne Bildgebungstechniken wie Magnetresonanztomographie und Computertomographie ermöglicht. Mit diesen Methoden wurden die normale Anatomie der PV definiert und ihre Varianten wie zusätzliche rechtsseitige m...

  13. Coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food of animal origin--phenotypic and genotypic antibiotic resistance.

    Science.gov (United States)

    Chajęcka-Wierzchowska, Wioleta; Zadernowska, Anna; Nalepa, Beata; Sierpińska, Magda; Łaniewska-Trokenheim, Łucja

    2015-04-01

    The aim of this work was to study the pheno- and genotypical antimicrobial resistance profile of coagulase negative staphylococci (CoNS) isolated from 146 ready-to-eat food of animal origin (cheeses, cured meats, sausages, smoked fishes). 58 strains were isolated, they were classified as Staphylococcus xylosus (n = 29), Staphylococcus epidermidis (n = 16); Staphylococcus lentus (n = 7); Staphylococcus saprophyticus (n = 4); Staphylococcus hyicus (n = 1) and Staphylococcus simulans (n = 1) by phenotypic and genotypic methods. Isolates were tested for resistance to erythromycin, clindamycin, gentamicin, cefoxitin, norfloxacin, ciprofloxacin, tetracycline, tigecycline, rifampicin, nitrofurantoin, linezolid, trimetoprim, sulphamethoxazole/trimethoprim, chloramphenicol, quinupristin/dalfopristin by the disk diffusion method. PCR was used for the detection of antibiotic resistance genes encoding: methicillin resistance--mecA; macrolide resistance--erm(A), erm(B), erm(C), mrs(A/B); efflux proteins tet(K) and tet(L) and ribosomal protection proteins tet(M). For all the tet(M)-positive isolates the presence of conjugative transposons of the Tn916-Tn1545 family was determined. Most of the isolates were resistant to cefoxitin (41.3%) followed by clindamycin (36.2%), tigecycline (24.1%), rifampicin (17.2%) and erythromycin (13.8%). 32.2% staphylococcal isolates were multidrug resistant (MDR). All methicillin resistant staphylococci harboured mecA gene. Isolates, phenotypic resistant to tetracycline, harboured at least one tetracycline resistance determinant on which tet(M) was most frequent. All of the isolates positive for tet(M) genes were positive for the Tn916-Tn1545 -like integrase family gene. In the erythromycin-resistant isolates, the macrolide resistance genes erm(C) or msr(A/B) were present. Although coagulase-negative staphylococci are not classical food poisoning bacteria, its presence in food could be of public health significance due to the possible spread of

  14. Antibacterial resistance of community-acquired respiratory tract pathogens recovered from patients in Latin America: results from the PROTEKT surveillance study (1999-2000

    Directory of Open Access Journals (Sweden)

    Mendes C.

    2003-01-01

    Full Text Available PROTEKT (Prospective Resistant Organism Tracking and Epidemiology for the Ketolide Telithromycin is a global surveillance study established in 1999 to monitor antibacterial resistance of respiratory tract organisms. Thirteen centers from Argentina, Brazil and Mexico participat ed during 1999-2000; they collected 1,806 isolates (Streptococcus pneumoniae 518, Haemophilus influenzae 520, Moraxella catarrhalis 140, Staphylococcus aureus 351, S. pyogenes 277. Overall, 218 (42.1% of the S. pneumoniae isolates had reduced susceptibility to penicillin, 79 (15.3% were penicillin-resistant and 79 (15.3% were erythromycin-resistant. Mexico had the highest prevalence of penicillin (76.5% and erythromycin (31.2% resistance. Of 77 erythromycin-resistant S. pneumoniae tested for resistance genotype, 43 possessed mef(A, 33 possessed erm(B and 1 possessed both erm(B and mef(A mechanism. All S. pneumoniae isolates were fully susceptible to telithromycin, linezolid, teicoplanin and vancomycin. Among H. influenzae isolates, 88 (16.9% produced b-lactamase, ranging from 11% (Brazil to 24.5% (Mexico. Among M. catarrhalis isolates, 138 (98.6% produced b-lactamase. Twenty-four (8.7% of the S. pyogenes isolates were erythromycin-resistant; resistance being attributable to mefA (n=18, ermTR (n=5 and ermB (n=1. All H. influenzae, M. catarrhalis and S. pyogenes were fully susceptible to telithromycin. Methicillin resistance was found in 26.5% of the S. aureus isolates (Argentina 15%; Mexico 20%; Brazil 31.3%. Telithromycin was effective against 97.7% of methicillin-susceptible isolates. PROTEKT confirms that antibacterial resistance is an emerging problem in Latin America. The previously reported high levels of pneumococcal resistance to the b-lactam and macrolides were exceeded. New agents that do not induce resistance or that exert low selective pressure, e.g. telithromycin, are essential to safeguard future antibacterial efficacy.

  15. Quantum interference in the dielectronic recombination of heavy highly charged ions

    OpenAIRE

    González Martínez, Antonio Javier

    2005-01-01

    Die Photorekombination hochgeladener Quecksilberionen wurde mittels einer Elektronenstrahlionenfalle (electron beam ion trap) experimentell untersucht. Ein Elektronenstrahl variabler Energie überstrich den Bereich 45-54 keV, in dem die KLL Resonanzen der Hg75+...78+ -Ionen liegen. Zum Nachweis der Photorekombination diente die dabei erzeugte Strahlung. Dies ermöglichte es, die Anregungsenergien dieser Resonanzen zustandsselektiv mit Messfehlern kleiner als ± 5 eV zu bestimmen. Diese Genauigke...

  16. Архитектурная прогулка / Любовь Маклеренко

    Index Scriptorium Estoniae

    Маклеренко, Любовь

    2010-01-01

    Tutvustatakse 2009. aastal ehitatud hooneid Tallinnas: Tammsaare Ärimaja (arhitekt Andres Kariste), Nordea maja (arhitektid Emil Urbel ja Indrek Erm), Scala City büroohoone (Nord Projekt), Marienthali keskus (arhitektid Andri Kirsima ja Hannes Niineväli), Metro Plaza ja tornidega maja Rotermanni kvartalis (KOKO Arhitektid), Tehnikaülikooli raamatukoguhoone (arhitektid Eero Endjärv, Priit Pent, Mattias Agabus, Illimar Truverk), kortermaja Aia tänaval (Kosmos)

  17. 3He-4He-Entmischungskryostat zur Kopplung ultrakalter Atome und supraleitender Strukturen: Konzeption und Herstellung des supraleitenden Spulensystems

    OpenAIRE

    Vergien, Petra Simone

    2014-01-01

    Ein vielversprechender Weg zur Realisierung von Prozessoren der Quanteninformationsverarbeitung besteht in der Verwendung von supraleitenden Qubits. Durch deren kurze Schaltzeiten wird die effiziente Durchführung von Quantenalgorithmen ermöglicht. Der gravierenste Nachteil solcher Strukturen liegt allerdings in ihrer kurzen Kohärenzeit im µs-Bereich. Für die Speicherung der Informationen muss somit nach alternativen Strategien gesucht werden. Eine Möglichkeit hierfür besteht in der Verwendung...

  18. Herpesvirus saimiri basierte Vektoren für die somatische Gentherapie der Rheumatoiden Arthritis

    OpenAIRE

    Wieser, Carsten

    2004-01-01

    Auf Herpesvirus saimiri basierende Vektoren für die Gentherapie können eine Alternative zu etablierten Vektoren darstellen, insbesondere aufgrund ihrer hohen Klonierungskapazität, der Infizierbarkeit verschiedenster Zelltypen und ihrer Fähigkeit zur episomalen Persistenz. Viele Erkrankungen erfordern eine steuerbare Expression eingebrachter Gene, wobei sich der Einsatz regulierbarer eukaryonter Expressionssysteme als hilfreich erwies. Um dies auch mit HVS-Vektoren zu ermöglichen, wurde zunäch...

  19. Ühinemise ja iseolemise vastuseis. Ideaalid ja reaalsus / Rein Tootmaa

    Index Scriptorium Estoniae

    Tootmaa, Rein

    2001-01-01

    Mängufilmid "Keiser ja palgamõrtsukas" ("Jing ke ci qin wang") : režissöör Chen Kaige : Jaapan-Hiina-Prantsusmaa 1999 ja "Tiiger ja draakon" ("Wo hu chang long") : režissöör Ang Lee : Hiina-Hongkong-Ameerika Ühendriigid 2000. Lisa : Aare Ermeli koostatud Chen Kaige ja Ang Lee loomingu lühikokkuvõtted. Kokkuvõte inglise keeles lk. 128

  20. Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal

    OpenAIRE

    Sousa, Margarida; Silva, Nuno; Igrejas, Gilberto; Sargo, Roberto; Benito, Daniel; Gómez, Paula; Lozano, Carmen; Manageiro, Vera; Torres, Carmen; Caniça, Manuela; Poeta, Patrícia

    2016-01-01

    Wild animal populations in contact with antimicrobials and antimicrobial resistant bacteria that are daily released into the environment are able to become unintentional hosts of these resistant microorganisms. To clarify this issue, our study evaluated the presence of antibiotic resistance determinants on coagulase-negative staphylococci recovered from birds of prey and studied their genetic relatedness by pulsed-field gel electrophoresis (PFGE). The unusual vga(A) and erm(T) genes, which co...