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Sample records for enrichment method medida

  1. Method of deuterium isotope separation and enrichment

    International Nuclear Information System (INIS)

    Benson, S.W.

    1978-01-01

    A method of separating deuterium, i.e., heavy hydrogen, from certain naturally occurring sources using tuned infrared lasers to selectively decompose specified classes of organic molecules (i.e., RX) into enriched molecular products containing deuterium atoms is described. The deuterium containing molecules are easily separated from the starting material by absorption, distillation or other simple chemical separation techniques and methods. After evaporation such deuterium containing molecules can be burned to form water with an enriched deuterium content or pyrolyzed to form hydrogen gas with an enriched deuterium content. The undecomposed molecules and the other reaction products which are depleted of their deuterium containing species can be catalytically treated, preferably using normal water, to restore the natural abundance of deuterium and such restored molecules can then be recycled

  2. Method of deuterium isotope separation and enrichment

    International Nuclear Information System (INIS)

    Benson, S.W.

    1979-01-01

    A method is described for separating and enriching deuterium containing molecules comprising the steps of: providing a source of organic molecules containing a normal abundance of deuterium atoms, the organic molecules having a structural formula RX, in which R is an organic radical selected from ethyl, isopropyl, t-butyl and 3-cyclopentenyl, and in which X is selected from F, Cl, Br and OH, and wherein R represents 3-cyclopentenyl, X may additionally represent H; exposing the molecules to the radiation of at least one pulsed infrared laser source which has been specifically tuned and focussed to selectively decompose RX molecules containing deuterium to form an enriched olefin specie containing deuterium, and HX; and separating the deuterium enriched olefin specie from the undecomposed deuterium depleted RX molecules and HX. (author)

  3. Method of deuterium isotope separation and enrichment

    International Nuclear Information System (INIS)

    Benson, S.W.

    1980-01-01

    A method of deuterium isotope separation and enrichment using infrared laser technology in combination with chemical processes for treating and recycling the unreacted and deuterium-depleted starting materials is described. Organic molecules of the formula RX (where R is an ethyl, isopropyl, t-butyl, or cyclopentenyl group and X is F, Cl, Br or OH) containing a normal abundance of hydrogen and deuterium are exposed to intense laser infrared radiation. An olefin containing deuterium (olefin D) will be formed, along with HX. The enriched olefin D can be stripped from the depleted stream of RX and HX, and can be burned to form enriched water or pyrolyzed to produce hydrogen gas with elevated deuterium content. The depleted RX is decomposed to olefins and RX, catalytically exchanged with normal water to restore the deuterium content to natural levels, and recombined to form RX which can be recycled. (LL)

  4. Method for the enrichment of isotopes

    International Nuclear Information System (INIS)

    Schuster, E.; Kersting, A.; Gebauhr, W.

    1976-01-01

    A method for the enrichment of isotopes, especially of uranium compounds, is described, working according to the principle of selective excitation by laser beams. As the starting compound, UF 6 is mentioned; the reaction partness are atomic gases (hydrogen, oxygen, nitrogen) or radicals (CH 3 , CHO). According to the invention, the gas mixture flows through the reaction space with a velocity so large that in leaving it, recombination of the reaction partner will already take place again. In this way, competing thermal reactions will be avoided and energy transfer fo excited 235 U molecules to non-excited 238 U molecules is eliminated to a large extent. A suitable equipment for implementing the method is described. (UWI) [de

  5. Método computadorizado para medida da acuidade visual A computerized method for visual acuity assessment

    Directory of Open Access Journals (Sweden)

    Patrícia Katayama Kjaer Arippol

    2006-12-01

    Full Text Available OBJETIVO: Elaborar e validar teste computadorizado para medida da acuidade visual de escolares. MÉTODOS: Foi elaborado teste computadorizado para determinação da acuidade visual utilizando os padrões das tabelas logarítmicas impressas adotadas na clínica oftalmológica. Foram avaliados 90 alunos da primeira série do ensino básico, oito estudantes do curso de Tecnologia Oftálmica da UNIFESP-EPM e 10 pacientes do ambulatório de Estrabismo do Departamento de Oftalmologia da UNIFESP-EPM. Todos os sujeitos foram avaliados pelo mesmo examinador e submetidos ao exame de acuidade visual monocular, pela tabela logarítmica de optotipos E impressa e do novo teste computadorizado no mesmo momento. Os participantes forneceram os seus consentimentos após esclarecimento. RESULTADOS: As análises estatísticas revelaram correlação excelente (r>0,75 entre os dois métodos, apesar da leve tendência apresentada pelo teste computadorizado em superestimar a acuidade visual quando comparado com o padrão-ouro. O teste computadorizado apresentou sensibilidade de 100% e especificidade de 94%. CONCLUSÕES: Os resultados obtidos nos permitem dizer que o teste computadorizado pode ser utilizado como novo recurso para triagem da qualidade visual dos escolares, por ser método rápido, de fácil aplicação, barato, automático e atrativo para as crianças. A automatização desvincula o aplicador da interpretação das respostas dadas pelo aluno testado, garante padronização do procedimento, que favorece as análises de acompanhamento e pode ser realizado por diferentes examinadores. Para melhor compreensão da efetividade do teste como instrumento de triagem visual, seria interessante instituí-lo nas escolas do curso básico, após treinamento dos professores para sua aplicação.PURPOSE: To elaborate and to validate a computerized test for visual acuity screening of school-age children. METHODS: We have created a computerized test for visual acuity

  6. Method and apparatus for enrichment or upgrading heavy water

    International Nuclear Information System (INIS)

    Butler, J.P.; Hammerli, M.

    1979-01-01

    A method and apparatus for upgrading and final enrichment of heavy water are described, comprising means for contacting partially enriched heavy water feed in a catalyst column with hydrogen gas (essentially D 2 ) originating in an electrolysis cell so as to enrich the feed water with deuterium extracted from the electrolytic hydrogen gas and means for passing the deuterium enriched water to the electrolysis cell. (author)

  7. Method for separation and enrichment of isotopes

    International Nuclear Information System (INIS)

    Kakihana, H.; Miyamatsu, T.

    1977-01-01

    Boron or uranium isotopes can be chemically separated and enriched with high speed and with high separating efficiency by using weakly basic anion exchange fibers having a diameter of not more than 100 μ, an aspect ratio of at least 5 and an exchange capacity of at least 2 meq/g-dry fiber, which are packed in a column at a specific volume of 2.0 to 20.0 ml/g-dry fiber

  8. Determination of uranium enrichment by using gamma-spectrometric methods

    International Nuclear Information System (INIS)

    Kutnyj, D.V.; Telegin, Yu.N.; Odejchuk, N.P.; Mikhailov, V.A.; Tovkanets, V.E.

    2009-01-01

    By using commercial analysis programs MGAU (LLNL, USA) and FRAM (LANL, USA) the summary error of gamma-spectrometric uranium enrichment measurements was investigated. Uranium samples with enrichments of 0,71; 4,46 and 20,1 % were measured. The coaxial high purity germanium detector (type GC) and the planar germanium detector (type LEGe) were used as gamma-radiation detectors. It was shown that experimental equipment and mathematical software available in NSC KIPT allow us to measure uranium enrichment by nondestructive method with accuracy of not worse than 2%.

  9. Selective adsorption-desorption method for the enrichment of krypton

    International Nuclear Information System (INIS)

    Yuasa, Y.; Ohta, M.; Watanabe, A.; Tani, A.; Takashima, N.

    1975-01-01

    Selective adsorption-desorption method has been developed as an effective means of enriching krypton and xenon gases. A seriesof laboratory-scale tests were performed to provide some basic data of the method when applied to off-gas streams of nuclear power plants. For the first step of the enrichment process of the experiments, krypton was adsorbed on solid adsorbents from dilute mixtures with air at temperatures ranging from -50 0 C to -170 0 C. After the complete breakthrough was obtained, the adsorption bed was evacuated at low temperature by a vacuum pump. By combining these two steps krypton was highly enriched on the adsorbents, and the enrichment factor for krypton was calculated as the product of individual enrichment factors of each step. Two types of adsorbents, coconut charcoal and molecular sieves 5A, were used. Experimental results showed that the present method gave the greater enrichment factor than the conventional method which used selective adsorption step only. (U.S.)

  10. Improved verification methods for safeguards verifications at enrichment plants

    International Nuclear Information System (INIS)

    Lebrun, A.; Kane, S. C.; Bourva, L.; Poirier, S.; Loghin, N. E.; Langlands, D.

    2009-01-01

    The International Atomic Energy Agency (IAEA) has initiated a coordinated research and development programme to improve its verification methods and equipment applicable to enrichment plants. The programme entails several individual projects to meet the objectives of the IAEA Safeguards Model Approach for Gas Centrifuge Enrichment Plants updated in 2006. Upgrades of verification methods to confirm the absence of HEU (highly enriched uranium) production have been initiated and, in particular, the Cascade Header Enrichment Monitor (CHEM) has been redesigned to reduce its weight and incorporate an electrically cooled germanium detector. Such detectors are also introduced to improve the attended verification of UF 6 cylinders for the verification of the material balance. Data sharing of authenticated operator weighing systems such as accountancy scales and process load cells is also investigated as a cost efficient and an effective safeguards measure combined with unannounced inspections, surveillance and non-destructive assay (NDA) measurement. (authors)

  11. Improved verification methods for safeguards verifications at enrichment plants

    Energy Technology Data Exchange (ETDEWEB)

    Lebrun, A.; Kane, S. C.; Bourva, L.; Poirier, S.; Loghin, N. E.; Langlands, D. [Department of Safeguards, International Atomic Energy Agency, Wagramer Strasse 5, A1400 Vienna (Austria)

    2009-07-01

    The International Atomic Energy Agency (IAEA) has initiated a coordinated research and development programme to improve its verification methods and equipment applicable to enrichment plants. The programme entails several individual projects to meet the objectives of the IAEA Safeguards Model Approach for Gas Centrifuge Enrichment Plants updated in 2006. Upgrades of verification methods to confirm the absence of HEU (highly enriched uranium) production have been initiated and, in particular, the Cascade Header Enrichment Monitor (CHEM) has been redesigned to reduce its weight and incorporate an electrically cooled germanium detector. Such detectors are also introduced to improve the attended verification of UF{sub 6} cylinders for the verification of the material balance. Data sharing of authenticated operator weighing systems such as accountancy scales and process load cells is also investigated as a cost efficient and an effective safeguards measure combined with unannounced inspections, surveillance and non-destructive assay (NDA) measurement. (authors)

  12. Enriching behavioral ecology with reinforcement learning methods.

    Science.gov (United States)

    Frankenhuis, Willem E; Panchanathan, Karthik; Barto, Andrew G

    2018-02-13

    This article focuses on the division of labor between evolution and development in solving sequential, state-dependent decision problems. Currently, behavioral ecologists tend to use dynamic programming methods to study such problems. These methods are successful at predicting animal behavior in a variety of contexts. However, they depend on a distinct set of assumptions. Here, we argue that behavioral ecology will benefit from drawing more than it currently does on a complementary collection of tools, called reinforcement learning methods. These methods allow for the study of behavior in highly complex environments, which conventional dynamic programming methods do not feasibly address. In addition, reinforcement learning methods are well-suited to studying how biological mechanisms solve developmental and learning problems. For instance, we can use them to study simple rules that perform well in complex environments. Or to investigate under what conditions natural selection favors fixed, non-plastic traits (which do not vary across individuals), cue-driven-switch plasticity (innate instructions for adaptive behavioral development based on experience), or developmental selection (the incremental acquisition of adaptive behavior based on experience). If natural selection favors developmental selection, which includes learning from environmental feedback, we can also make predictions about the design of reward systems. Our paper is written in an accessible manner and for a broad audience, though we believe some novel insights can be drawn from our discussion. We hope our paper will help advance the emerging bridge connecting the fields of behavioral ecology and reinforcement learning. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

  13. Method of enriching oil shale concentrate

    Energy Technology Data Exchange (ETDEWEB)

    Larsson, M

    1942-02-14

    The method is characterized by producing one concentrate first and then this concentrate, in water solution using suitable apparatus, is separated by settling into one heavier bottom portion, rich in mineral matter, and into a lighter slime containing concentrated organic substance.

  14. Method for enrichment by dual temperature exchange

    International Nuclear Information System (INIS)

    Spevack, J.S.

    1977-01-01

    In dual temperature systems utilizing different fluid materials in liquid and gas phases separable from each other (for example H 2 O and H 2 S), the phases are contacted with each other at a relatively hot temperature. Herein combinations of method and means are provided by which the gas is conditioned by raising its temperature and humidity principally by heat derived from the cooling and dehumidification of said gas. Special provisions are made in the combinations for transferring said heat and for the conditioning of the gas with high efficiency; and for economically controlling the temperature of the condensate resulting from the dehumidification of the gas to adapt it for particular uses in the system. Method and means are provided for such liquid gas contacting systems for efficiently stripping or separating dissolved gas from the effluent liquid and returning one of the so separated materials to the system

  15. Experimental study on enriching 12C by centrifuge method

    International Nuclear Information System (INIS)

    Xiao Huaxian

    1994-07-01

    The diamond made from the highly enriched 12 C, whose thermal conductivity and electric insulativity are much better than that of natural diamond, has widely uses in new and high technology. In many enriching 12 C methods, the gas centrifuge method is superior to others. After selecting the appropriate process gas and solving key problems, such as feed and extract, the separation experiments are performed by a single stage of centrifuge. To increase the separation capacity of single machine, various parameters in the centrifugal separation are optimized, and appropriate mechanical drive, thermal drive, hold-up and process parameters are selected. The optimal operating condition of single machine is also obtained in the cascade. Thus, highly enriched 12 C is produced in the centrifuge cascade

  16. Method and device for the enrichment of isotopes

    International Nuclear Information System (INIS)

    Stehle, H.

    1976-01-01

    A variation of a method for isotope enrichment by laser radiation is proposed which improves the selectivity and with it the economy of the method by eliminating undesired reactions caused by thermal activation. The method according to the invention is applied discontinuously in three steps: The isotope mixture and the reacting agents are fed to a vessel, a laser beam is passed through, and the contents are emptied into a vacuum tank while expanding adiabatically. The time steps are controlled. The method is explained using the example of separating an isotope mixture of UF 6 . (UWI) [de

  17. Enrichment of gadolinium-157 and gadolinium-155 by laser method

    International Nuclear Information System (INIS)

    Xinjun, Su; Xiaowei, Zhang; Zhiquan, Li

    2008-01-01

    Laser isotope separation experiments of gadolinium by atomic vapor method have been performed. Gadolinium-157 and gadolinium-155 were selectively photoionized by means of three linearly polarized dye lasers, the excitation process of which is based on the polarization selection rules. Gramme-magnitude of enriched gadolinium was obtained and the total abundance of gadolinium-157 and gadolinium-155 was in excess of 80%, and the product rating exceeded 200 mg/h. (author)

  18. Method of producing deuterium-oxide-enriched water

    International Nuclear Information System (INIS)

    Mandel, H.

    1976-01-01

    A method and apparatus for producing deuterium-oxide-enriched water (e.g., as a source of deuterium-rich gas mixtures) are disclosed wherein the multiplicity of individual cooling cycles of a power plant are connected in replenishment cascade so that fresh feed water with a naturally occurring level of deuterium oxide is supplied to replace the vaporization losses, sludge losses and withdrawn portion of water in a first cooling cycle, the withdrawn water being fed as the feed water to the subsequent cooling cycle or stage and serving as the sole feed-water input to the latter. At the end of the replenishment-cascade system, the withdrawn water has a high concentration of deuterium oxide and may serve as a source of water for the production of heavy water or deuterium-enriched gas by conventional methods of removing deuterium oxide or deuterium from the deuterium-oxide-enriched water. Each cooling cycle may form part of a thermal or nuclear power plant in which a turbine is driven by part of the energy and air-cooling of the water takes place in the atmosphere, e.g., in a cooling tower

  19. Quantitative mass spectrometric analysis of glycoproteins combined with enrichment methods.

    Science.gov (United States)

    Ahn, Yeong Hee; Kim, Jin Young; Yoo, Jong Shin

    2015-01-01

    Mass spectrometry (MS) has been a core technology for high sensitive and high-throughput analysis of the enriched glycoproteome in aspects of quantitative assays as well as qualitative profiling of glycoproteins. Because it has been widely recognized that aberrant glycosylation in a glycoprotein may involve in progression of a certain disease, the development of efficient analysis tool for the aberrant glycoproteins is very important for deep understanding about pathological function of the glycoprotein and new biomarker development. This review first describes the protein glycosylation-targeting enrichment technologies mainly employing solid-phase extraction methods such as hydrizide-capturing, lectin-specific capturing, and affinity separation techniques based on porous graphitized carbon, hydrophilic interaction chromatography, or immobilized boronic acid. Second, MS-based quantitative analysis strategies coupled with the protein glycosylation-targeting enrichment technologies, by using a label-free MS, stable isotope-labeling, or targeted multiple reaction monitoring (MRM) MS, are summarized with recent published studies. © 2014 The Authors. Mass Spectrometry Reviews Published by Wiley Periodicals, Inc.

  20. Estudo comparativo entre dois métodos de medida da distância interpupilar Comparative study between two measurement methods of interpupillary distance

    Directory of Open Access Journals (Sweden)

    Daniela Vieira Roehe

    2008-04-01

    Full Text Available OBJETIVO: O objetivo do estudo é realizar as medidas da Distância Interpupilar (DIP entre a régua milimetrada e o autorrefrator e comparar os resultados entre si. MÉTODOS: Cento e trinta e cinco pacientes foram submetidos à avaliação da DIP. Cada paciente foi examinado pelo mesmo examinador, com dois métodos: régua milimetrada e autorrefrator. RESULTADOS: Houve boa concordância entre as medidas apuradas pelos dois métodos, não apresentando diferença estatísticamente significante entre as médias e variabilidades. CONCLUSÃO: A inexatidão presente no método da régua milimetrada pode responder pelos casos com diferenças clinicamente significantes, porém proporcionalmente estes casos não invalidam a reprodutibilidade do autorrefrator.PURPOSE: There are several methods to measure the Interpupilary Distance (IPD, but the method used in most office is the milimetric ruler. Currently, the autorrefractor is used routinely to find for the subjective evaluation of the refractive error, but the IPD from the autorrefractor is often not compared with the traditional method of measurement.The objective of the study is to achieve the measures of IPD between the two methods and compare the results with each other. METHODS: One hundred and thirty-five patients underwent the assessment of the DIP. Each patient was examined by the same examiner, with two methods: milimetric ruler and autorrefractor. RESULTS: There was good agreement between the measures cleared by the two methods, showing no statistically significant difference between mean and variability. CONCLUSION: The inaccuracy in the method of the present milimetric ruler can answer the cases with clinically significant differences, however proportionately these cases do not invalidate the reproducibility of the autorrefractor.

  1. Method and apparatus for measuring enrichment of UF6

    Science.gov (United States)

    Hill, Thomas Roy [Santa Fe, NM; Ianakiev, Kiril Dimitrov [Los Alamos, NM

    2011-06-07

    A system and method are disclosed for determining the enrichment of .sup.235U in Uranium Hexafluoride (UF6) utilizing synthesized X-rays which are directed at a container test zone containing a sample of UF6. A detector placed behind the container test zone then detects and counts the X-rays which pass through the container and the UF6. In order to determine the portion of the attenuation due to the UF6 gas alone, this count rate may then be compared to a calibration count rate of X-rays passing through a calibration test zone which contains a vacuum, the test zone having experienced substantially similar environmental conditions as the actual test zone. Alternatively, X-rays of two differing energy levels may be alternately directed at the container, where either the container or the UF6 has a high sensitivity to the difference in the energy levels, and the other having a low sensitivity.

  2. Device and method to enrich and process heavy water

    International Nuclear Information System (INIS)

    Hammerli, M.M.; Butler, J.P.

    1979-01-01

    A device to process and enrich heavy water is proposed which is based on a combined electrolysis catalyst exchange system in which a D 2 O enrichment of more than 99.8% is achieved in the end stage. Water partly enriched with D 2 -containing hydrogen gas from an electrolysis cell is brought into contact in a catalyst column. The water is further enriched here with deuterium. It is then fed to the electrolysis cell. Details of the apparatus are closely described. (UWI) [de

  3. Coal Enrichment Methods by Using Microorganisms and Their Metabolites

    Directory of Open Access Journals (Sweden)

    Małgorzata Deska

    2018-03-01

    Full Text Available The aim of this study is to review the literature on the methods of low-rank coal enrichment by using microorganisms and their metabolites. Effective bio-beneficiation technologies for low-rank coals in the future are also suggested throughout this paper. An extensive literature review highlights recent advances in bio-beneficiation technologies for low rank coals. This paper presents the state of the art in the field of the bio-beneficiation technology - carbon leaching with the aid of microorganisms, especially fungi. The knowledge of the low-rank coals leaching is an important step to meet the carbon eco-requirements and improve the economics of mining companies. There are several reasons to investigate microbial activities towards coal. This paper presents the current state of knowledge concerning bioleaching of coal. Thus, in view of the increasing importance of hard coal as a raw material and energy source, it seems hopeful to study the potential of microorganisms to modify the low-rank coal structure.

  4. Development of quality assurance methods for low enriched fuel assemblies

    International Nuclear Information System (INIS)

    Woolstenhulme, N.E.; Moore, G.A.; Perez, D.M.; Wachs, D.M.

    2010-01-01

    As the Reduced Enrichment for Research and Test Reactors (RERTR) fuel development program has furthered the technology of low enriched uranium fuels, much effort has been expended to specify requirements, perform appropriate inspections, and to qualify experimental fuel plates and assemblies for irradiation. A great deal of consideration has been given to generate examinations and criteria that are both applicable to the unique fuel types being developed and consistent with industry practices for inspecting plate-type reactor fuel. Recent developments in quality assurance (QA) methodologies have given a heightened confidence in satisfactory fuel plate performance. At the same time, recommendations are given to further develop a system suitable for the testing and acceptance of production fuel elements containing low enriched uranium fuels. (author)

  5. Laboratory studies of 235U enrichment by chemical separation methods

    International Nuclear Information System (INIS)

    Daloisi, P.J.; Orlett, M.J.; Tracy, J.W.; Saraceno, A.J.

    1976-01-01

    Laboratory experiments on 235 U enrichment processes based on column redox ion exchange, electrodialysis, and gas exchange chromatography performed from August 1972 to September 1974 are summarized. Effluent from a 50 to 50 weight mixture of U +4 and U +6 (as UO 2 2+ ), at a total uranium concentration of 5 mg U per ml in 0.25N H 2 SO 4 -0.03N NaF solution, passing through a 100 cm length cation exchange column at 0.5 ml/min flow rates, was enriched in 235 U by 1.00090 +- .00012. The enriched fraction was mostly in the +6 valence form while the depleted fraction was U +4 retained on the resin. At flow rates of 2 ml/min, the enrichment factor decreases to 1.00033 +- .00003. In the electrodialysis experiments, the fraction of uranium diffusing through the membranes (mostly as +6 valence state) in 4.2 hours is enriched in 235 U by 1.00096 +- .00012. Gas exchange chromatography tests involved dynamic and static exposure of UF 6 over NaF. In dynamic tests, no significant change in isotopic abundance occurred in the initial one-half weight cut of UF 6 . The measured relative 235 U/ 238 U mole ratios were 1.00004 +- .00004 for these runs. In static runs, enrichment became evident. For the NaF(UF 6 )/sub x/-UF 6 system, there is 235 U depletion in the gas phase, with a single-stage factor of 1.00033 at 100 0 C and 1.00025 at 25 0 C after 10 days of equilibration. The single-stage or unit holdup time is impractically long for all three chemical processes

  6. Nuclear material enrichment identification method based on cross-correlation and high order spectra

    International Nuclear Information System (INIS)

    Yang Fan; Wei Biao; Feng Peng; Mi Deling; Ren Yong

    2013-01-01

    In order to enhance the sensitivity of nuclear material identification system (NMIS) against the change of nuclear material enrichment, the principle of high order statistic feature is introduced and applied to traditional NMIS. We present a new enrichment identification method based on cross-correlation and high order spectrum algorithm. By applying the identification method to NMIS, the 3D graphs with nuclear material character are presented and can be used as new signatures to identify the enrichment of nuclear materials. The simulation result shows that the identification method could suppress the background noises, electronic system noises, and improve the sensitivity against enrichment change to exponential order with no system structure modification. (authors)

  7. Enrichment Assay Methods Development for the Integrated Cylinder Verification System

    International Nuclear Information System (INIS)

    Smith, Leon E.; Misner, Alex C.; Hatchell, Brian K.; Curtis, Michael M.

    2009-01-01

    International Atomic Energy Agency (IAEA) inspectors currently perform periodic inspections at uranium enrichment plants to verify UF6 cylinder enrichment declarations. Measurements are typically performed with handheld high-resolution sensors on a sampling of cylinders taken to be representative of the facility's entire product-cylinder inventory. Pacific Northwest National Laboratory (PNNL) is developing a concept to automate the verification of enrichment plant cylinders to enable 100 percent product-cylinder verification and potentially, mass-balance calculations on the facility as a whole (by also measuring feed and tails cylinders). The Integrated Cylinder Verification System (ICVS) could be located at key measurement points to positively identify each cylinder, measure its mass and enrichment, store the collected data in a secure database, and maintain continuity of knowledge on measured cylinders until IAEA inspector arrival. The three main objectives of this FY09 project are summarized here and described in more detail in the report: (1) Develop a preliminary design for a prototype NDA system, (2) Refine PNNL's MCNP models of the NDA system, and (3) Procure and test key pulse-processing components. Progress against these tasks to date, and next steps, are discussed.

  8. Enrichment Assay Methods Development for the Integrated Cylinder Verification System

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Leon E.; Misner, Alex C.; Hatchell, Brian K.; Curtis, Michael M.

    2009-10-22

    International Atomic Energy Agency (IAEA) inspectors currently perform periodic inspections at uranium enrichment plants to verify UF6 cylinder enrichment declarations. Measurements are typically performed with handheld high-resolution sensors on a sampling of cylinders taken to be representative of the facility's entire product-cylinder inventory. Pacific Northwest National Laboratory (PNNL) is developing a concept to automate the verification of enrichment plant cylinders to enable 100 percent product-cylinder verification and potentially, mass-balance calculations on the facility as a whole (by also measuring feed and tails cylinders). The Integrated Cylinder Verification System (ICVS) could be located at key measurement points to positively identify each cylinder, measure its mass and enrichment, store the collected data in a secure database, and maintain continuity of knowledge on measured cylinders until IAEA inspector arrival. The three main objectives of this FY09 project are summarized here and described in more detail in the report: (1) Develop a preliminary design for a prototype NDA system, (2) Refine PNNL's MCNP models of the NDA system, and (3) Procure and test key pulse-processing components. Progress against these tasks to date, and next steps, are discussed.

  9. The method of water enrichment in oxygen-18

    International Nuclear Information System (INIS)

    Chmielewski, A.G.; Zakrzewska-Trznadel, G.; Van Hook, A.; Milievic, N.R.

    1996-01-01

    The process of membrane distillation has been used for water enrichment in oxygen-18. The hydrophobic PTFE membranes has been used as a boundary for liquids being at different temperatures on the each side of membrane. Example of obtained results have been shown

  10. Comparative study on gene set and pathway topology-based enrichment methods.

    Science.gov (United States)

    Bayerlová, Michaela; Jung, Klaus; Kramer, Frank; Klemm, Florian; Bleckmann, Annalen; Beißbarth, Tim

    2015-10-22

    Enrichment analysis is a popular approach to identify pathways or sets of genes which are significantly enriched in the context of differentially expressed genes. The traditional gene set enrichment approach considers a pathway as a simple gene list disregarding any knowledge of gene or protein interactions. In contrast, the new group of so called pathway topology-based methods integrates the topological structure of a pathway into the analysis. We comparatively investigated gene set and pathway topology-based enrichment approaches, considering three gene set and four topological methods. These methods were compared in two extensive simulation studies and on a benchmark of 36 real datasets, providing the same pathway input data for all methods. In the benchmark data analysis both types of methods showed a comparable ability to detect enriched pathways. The first simulation study was conducted with KEGG pathways, which showed considerable gene overlaps between each other. In this study with original KEGG pathways, none of the topology-based methods outperformed the gene set approach. Therefore, a second simulation study was performed on non-overlapping pathways created by unique gene IDs. Here, methods accounting for pathway topology reached higher accuracy than the gene set methods, however their sensitivity was lower. We conducted one of the first comprehensive comparative works on evaluating gene set against pathway topology-based enrichment methods. The topological methods showed better performance in the simulation scenarios with non-overlapping pathways, however, they were not conclusively better in the other scenarios. This suggests that simple gene set approach might be sufficient to detect an enriched pathway under realistic circumstances. Nevertheless, more extensive studies and further benchmark data are needed to systematically evaluate these methods and to assess what gain and cost pathway topology information introduces into enrichment analysis. Both

  11. Study on behaviors and performances of universal N-glycopeptide enrichment methods.

    Science.gov (United States)

    Xue, Yu; Xie, Juanjuan; Fang, Pan; Yao, Jun; Yan, Guoquan; Shen, Huali; Yang, Pengyuan

    2018-04-16

    Glycosylation is a crucial process in protein biosynthesis. However, the analysis of glycopeptides through MS remains challenging due to the microheterogeneity and macroheterogeneity of the glycoprotein. Selective enrichment of glycopeptides from complex samples prior to MS analysis is essential for successful glycoproteome research. In this work, we systematically investigated the behaviors and performances of boronic acid chemistry, ZIC-HILIC, and PGC of glycopeptide enrichment to promote understanding of these methods. We also optimized boronic acid chemistry and ZIC-HILIC enrichment methods and applied them to enrich glycopeptides from mouse liver. The intact N-glycopeptides were interpreted using the in-house analysis software pGlyco 2.0. We found that boronic acid chemistry in this study preferred to capture glycopeptides with high mannose glycans, ZIC-HILIC enriched most N-glycopeptides and did not show significant preference during enrichment and PGC was not suitable for separating glycopeptides with a long amino acid sequence. We performed a detailed study on the behaviors and performances of boronic acid chemistry, ZIC-HILIC, and PGC enrichment methods and provide a better understanding of enrichment methods for further glycoproteomics research.

  12. Methods to Enrich Exosomes from Conditioned Media and Biological Fluids.

    Science.gov (United States)

    Sharma, Shayna; Scholz-Romero, Katherin; Rice, Gregory E; Salomon, Carlos

    2018-01-01

    Exosomes are nano-vesicles which can transport a range of molecules including but not limited to proteins and miRNA. This ability of exosomes renders them useful in cellular communication often resulting in biological changes. They have several functions in facilitating normal biological processes such as immune responses and an involvement in pregnancy. However, they have also been linked to pathological conditions including cancer and pregnancy complications such as preeclampsia. An understanding for the role of exosomes in preeclampsia is based on the ability to purify and characterize exosomes. There have been several techniques proposed for the enrichment of exosomes such as ultracentrifugation, density gradient separation, and ultrafiltration although there is no widely accepted optimized technique. Here we describe a workflow for isolating exosomes from cell-conditioned media and biological fluids using a combination of centrifugation, buoyant density, and ultrafiltration approaches.

  13. Method for determining the wedge angle from the daily measurements made with the measurement enabled devices DC6; Metodo para la determinacion del angulo de cuna a partir de las medidas diarias realizadas con el dispositio de medida QC6

    Energy Technology Data Exchange (ETDEWEB)

    Marques Fraguela, E.; Suero Rodrigo, M. A.

    2011-07-01

    The aim of this paper is to present a method for determining the angle of the wedges virtual electron linear accelerator (ALE) Siemens Primus, from the daily measurements made with the measurement system PTW-QC6Plus and found to be sufficiently sensitive to determine variations of {+-} 1 of this parameter. In addition, we study the behavior statistically CUFLE angle over a year.

  14. Study on atmospheric hydrogen enrichment by cryopump method and isotope separation by gas chromatography

    International Nuclear Information System (INIS)

    Taniyama, Yuki; Momoshima, Noriyuki

    2001-01-01

    To obtain the information of source of atmospheric hydrogen tritium an analysis of tritium isotopes is thought to be effective. So an atmospheric hydrogen enrichment apparatus and a cryogenic gas chromatographic column were made. Experiments were carried out to study the performance of cryopump to enrich atmospheric hydrogen and the column to separate hydrogen isotopes that obtained by cryopump method. The cryopump was able to process about 1000 1 atmosphere and the column was able to separate hydrogen isotopes with good resolution. (author)

  15. Comparing Multi-Step IMAC and Multi-Step TiO2 Methods for Phosphopeptide Enrichment

    Science.gov (United States)

    Yue, Xiaoshan; Schunter, Alissa; Hummon, Amanda B.

    2016-01-01

    Phosphopeptide enrichment from complicated peptide mixtures is an essential step for mass spectrometry-based phosphoproteomic studies to reduce sample complexity and ionization suppression effects. Typical methods for enriching phosphopeptides include immobilized metal affinity chromatography (IMAC) or titanium dioxide (TiO2) beads, which have selective affinity and can interact with phosphopeptides. In this study, the IMAC enrichment method was compared with the TiO2 enrichment method, using a multi-step enrichment strategy from whole cell lysate, to evaluate their abilities to enrich for different types of phosphopeptides. The peptide-to-beads ratios were optimized for both IMAC and TiO2 beads. Both IMAC and TiO2 enrichments were performed for three rounds to enable the maximum extraction of phosphopeptides from the whole cell lysates. The phosphopeptides that are unique to IMAC enrichment, unique to TiO2 enrichment, and identified with both IMAC and TiO2 enrichment were analyzed for their characteristics. Both IMAC and TiO2 enriched similar amounts of phosphopeptides with comparable enrichment efficiency. However, phosphopeptides that are unique to IMAC enrichment showed a higher percentage of multi-phosphopeptides, as well as a higher percentage of longer, basic, and hydrophilic phosphopeptides. Also, the IMAC and TiO2 procedures clearly enriched phosphopeptides with different motifs. Finally, further enriching with two rounds of TiO2 from the supernatant after IMAC enrichment, or further enriching with two rounds of IMAC from the supernatant TiO2 enrichment does not fully recover the phosphopeptides that are not identified with the corresponding multi-step enrichment. PMID:26237447

  16. An improved cell recovery method for iron oxidizing bacterial (IOB) enrichments

    DEFF Research Database (Denmark)

    Yu, Ran; Graf, Joerg; Smets, Barth F.

    2008-01-01

    Two cell recovery methods for IOB enrichments were evaluated for DNA extraction and further PCR-based 16S rRNA gene clone library creation. One was a published method consisting of heating plus oxalic acid treatment and the other one was a new method based on enzymatic agarose digestion (using β...

  17. Present status of centrifuge method for uranium enrichment and PNC plant

    International Nuclear Information System (INIS)

    Nishikido, Yoshikazu

    1977-01-01

    Recentry, the tendency to adopt atomic energy owing to the oil shock, the delay in the construction of nuclear power stations worldwide, the uncertainty in the utilization of plutonium, and the prospect of supplying natural uranium are the situations affecting uranium enrichment. Anyway, the enrichment capacity in the world must be increased by the early years of 1980 s. The uranium enrichment technology by centrifugal method is being developed in various countries under strict control of informations, therefore the details are not known, but the general state in Great Britain, F.R. of Germany, Netherlands, U.S.A. and Japan is explained. The development of the centrifugal enrichment method in Japan was designated in 1972 as the national project aiming at operating the enriching plant with international competitive power by 1985. The PNC undertook the development work, and the research and development include the development of a centrifuge, cascade test, life span test, the development of the mass production technology, and safety test. The especially notable matter in this period was the rapid progress of a supercritical type centrifuge. It is judged that the technical basis for constructing a pilot plant has been established. The site for the pilot plant is being prepared now in the Ningyo Pass Mine, PNC, and the enrichment plant with 7000 centrifuges will be constructed there. The outline of the plant and the schedule for the construction are described. (Kako, I.)

  18. Enriched Meshfree Method for an Accurate Numerical Solution of the Motz Problem

    Directory of Open Access Journals (Sweden)

    Won-Tak Hong

    2016-01-01

    Full Text Available We present an enriched meshfree solution of the Motz problem. The Motz problem has been known as a benchmark problem to verify the efficiency of numerical methods in the presence of a jump boundary data singularity at a point, where an abrupt change occurs for the boundary condition. We propose a singular basis function enrichment technique in the context of partition of unity based meshfree method. We take the leading terms of the local series expansion at the point singularity and use them as enrichment functions for the local approximation space. As a result, we obtain highly accurate leading coefficients of the Motz problem that are comparable to the most accurate numerical solution. The proposed singular enrichment technique is highly effective in the case of the local series expansion of the solution being known. The enrichment technique that is used in this study can be applied to monotone singularities (of type rα with α<1 as well as oscillating singularities (of type rαsin⁡(ϵlog⁡r. It is the first attempt to apply singular meshfree enrichment technique to the Motz problem.

  19. Evaluation of different enrichment methods for pathogenic Yersinia species detection by real time PCR

    Science.gov (United States)

    2014-01-01

    Background Yersiniosis is a zoonotic disease reported worldwide. Culture and PCR based protocols are the most common used methods for detection of pathogenic Yersinia species in animal samples. PCR sensitivity could be increased by an initial enrichment step. This step is particularly useful in surveillance programs, where PCR is applied to samples from asymptomatic animals. The aim of this study was to evaluate the improvement in pathogenic Yersinia species detection using a suitable enrichment method prior to the real time PCR (rtPCR). Nine different enrichment protocols were evaluated including six different broth mediums (CASO, ITC, PSB, PBS, PBSMSB and PBSSSB). Results The analysis of variance showed significant differences in Yersinia detection by rtPCR according to the enrichment protocol used. These differences were higher for Y. pseudotuberculosis than for Y. enterocolitica. In general, samples incubated at lower temperatures yielded the highest detection rates. The best results were obtained with PBSMSB and PBS2. Application of PBSMSB protocol to free-ranging wild board samples improved the detection of Y. enterocolitica by 21.2% when compared with direct rtPCR. Y. pseudotuberculosis detection was improved by 10.6% when results obtained by direct rtPCR and by PBSMSB enrichment before rtPCR were analyzed in combination. Conclusions The data obtained in the present study indicate a difference in Yersinia detection by rtPCR related to the enrichment protocol used, being PBSMSB enrichment during 15 days at 4°C and PBS during 7 days at 4°C the most efficient. The use of direct rtPCR in combination with PBSMSB enrichment prior to rtPCR resulted in an improvement in the detection rates of pathogenic Yersinia in wild boar and could be useful for application in other animal samples. PMID:25168886

  20. Optimization of axial enrichment distribution for BWR fuels using scoping libraries and block coordinate descent method

    Energy Technology Data Exchange (ETDEWEB)

    Tung, Wu-Hsiung, E-mail: wstong@iner.gov.tw; Lee, Tien-Tso; Kuo, Weng-Sheng; Yaur, Shung-Jung

    2017-03-15

    Highlights: • An optimization method for axial enrichment distribution in a BWR fuel was developed. • Block coordinate descent method is employed to search for optimal solution. • Scoping libraries are used to reduce computational effort. • Optimization search space consists of enrichment difference parameters. • Capability of the method to find optimal solution is demonstrated. - Abstract: An optimization method has been developed to search for the optimal axial enrichment distribution in a fuel assembly for a boiling water reactor core. The optimization method features: (1) employing the block coordinate descent method to find the optimal solution in the space of enrichment difference parameters, (2) using scoping libraries to reduce the amount of CASMO-4 calculation, and (3) integrating a core critical constraint into the objective function that is used to quantify the quality of an axial enrichment design. The objective function consists of the weighted sum of core parameters such as shutdown margin and critical power ratio. The core parameters are evaluated by using SIMULATE-3, and the cross section data required for the SIMULATE-3 calculation are generated by using CASMO-4 and scoping libraries. The application of the method to a 4-segment fuel design (with the highest allowable segment enrichment relaxed to 5%) demonstrated that the method can obtain an axial enrichment design with improved thermal limit ratios and objective function value while satisfying the core design constraints and core critical requirement through the use of an objective function. The use of scoping libraries effectively reduced the number of CASMO-4 calculation, from 85 to 24, in the 4-segment optimization case. An exhausted search was performed to examine the capability of the method in finding the optimal solution for a 4-segment fuel design. The results show that the method found a solution very close to the optimum obtained by the exhausted search. The number of

  1. WaveSeq: a novel data-driven method of detecting histone modification enrichments using wavelets.

    Directory of Open Access Journals (Sweden)

    Apratim Mitra

    Full Text Available BACKGROUND: Chromatin immunoprecipitation followed by next-generation sequencing is a genome-wide analysis technique that can be used to detect various epigenetic phenomena such as, transcription factor binding sites and histone modifications. Histone modification profiles can be either punctate or diffuse which makes it difficult to distinguish regions of enrichment from background noise. With the discovery of histone marks having a wide variety of enrichment patterns, there is an urgent need for analysis methods that are robust to various data characteristics and capable of detecting a broad range of enrichment patterns. RESULTS: To address these challenges we propose WaveSeq, a novel data-driven method of detecting regions of significant enrichment in ChIP-Seq data. Our approach utilizes the wavelet transform, is free of distributional assumptions and is robust to diverse data characteristics such as low signal-to-noise ratios and broad enrichment patterns. Using publicly available datasets we showed that WaveSeq compares favorably with other published methods, exhibiting high sensitivity and precision for both punctate and diffuse enrichment regions even in the absence of a control data set. The application of our algorithm to a complex histone modification data set helped make novel functional discoveries which further underlined its utility in such an experimental setup. CONCLUSIONS: WaveSeq is a highly sensitive method capable of accurate identification of enriched regions in a broad range of data sets. WaveSeq can detect both narrow and broad peaks with a high degree of accuracy even in low signal-to-noise ratio data sets. WaveSeq is also suited for application in complex experimental scenarios, helping make biologically relevant functional discoveries.

  2. An evaluation of different target enrichment methods in pooled sequencing designs for complex disease association studies.

    Directory of Open Access Journals (Sweden)

    Aaron G Day-Williams

    Full Text Available Pooled sequencing can be a cost-effective approach to disease variant discovery, but its applicability in association studies remains unclear. We compare sequence enrichment methods coupled to next-generation sequencing in non-indexed pools of 1, 2, 10, 20 and 50 individuals and assess their ability to discover variants and to estimate their allele frequencies. We find that pooled resequencing is most usefully applied as a variant discovery tool due to limitations in estimating allele frequency with high enough accuracy for association studies, and that in-solution hybrid-capture performs best among the enrichment methods examined regardless of pool size.

  3. Sexualidade na terceira idade: medidas de prevenção para doenças sexualmente transmissíveis e AIDS Sexualidad en la tercera edad: medidas de prevención de enfermedades de transmisión sexual y SIDA Sexuality in the elderly: prevention methods for sexually transmissible illnesses and AIDS

    Directory of Open Access Journals (Sweden)

    Manoela Busato Mottin Maschio

    2011-09-01

    Full Text Available O objetivo deste estudo foi identificar as medidas de prevenção que os idosos estão utilizando para à prevenção das Doenças Sexualmente Transmissíveis e Síndrome da Imunodeficiência Adquirida. A pesquisa foi de caráter prospectivo, quantitativo e descritivo com uma amostragem intencional, realizada com 98 idosos. Foi aplicado um questionário com perguntas abertas e fechadas, relacionadas à vida sexual dos idosos frequentadores de uma instituição que desenvolve programas para a melhoria da qualidade de vida dos idosos no município de Curitiba, Paraná. Dos entrevistados 43%, relatam fazer uso de alguma medida de prevenção. A realização de programas de prevenção voltados para o atendimento de pessoas com 50 anos ou mais, deve estar atenta às questões de sexualidade no envelhecimento. Os idosos devem ser vistos como indivíduos que possuem desejos, necessidades sexuais e que fazem projetos para o futuro.El objetivo de este estudio es identificar medidas que los ancianos están utilizando para la prevención de enfermedades de transmisión sexual y el Enfermedades de Transmisión Sexual Síndrome de Inmunodeficiencia Adquirida. La encuesta fue de carácter prospectivo y cuantitativo con una muestra intencional, realizado con 98 personas de edad avanzada. Se aplicó un cuestionario con preguntas abiertas y cerradas, relacionadas con la vida sexual de los visitantes mayores de una institución que desarrolla programas para la mejora de la calidad de vida de las personas mayores en la ciudad de Curitiba, Paraná, Brasil. De los 43% de los encuestados informaron que usan de alguna medida de prevención. La ejecución de los programas de prevención se centró en la atención de personas mayores de 50 años o más, deben de estar atentos a las cuestiones de la sexualidad en el envejecimiento. Los ancianos deben ser vistos como individuos que tienen necesidades sexuales y deseos, que hacen proyectos para el futuro.This study aimed

  4. The advanced CECE process for enriching tritium by the chemical exchange method with a hydrophobic catalyst

    International Nuclear Information System (INIS)

    Kitamoto, Asashi; Shimizu, Masami; Masui, Takashi.

    1992-01-01

    The monothermal chemical exchange process with electrolysis, i.e., CECE process, was an effective method for enriching and removing tritium from tritiated water with low to middle level activity. The purpose of this study is to propose the theoretical background of the two-parameter evaluation method, which is based on a two-step isotope exchange reaction between hydrogen gas and liquid water, for improvement of the performance of a hydrophobic catalyst by a trickle bed-type column. Finally, a two-parameter method could attain the highest performance of isotope separation and the lowest liquid holdup for a trickle bed-type column. Therefore, this method will present some effective and practical procedures in scaling up a tritium enrichment process. The main aspect of the CECE process in engineering design and system evaluation was to develop the isotope exchange column with a high performance catalyst. (author)

  5. Modeling and Analysis Methods for an On-line Enrichment Monitor

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Leon E.; Jarman, Kenneth D.; Wittman, Richard S.; Zalavadia, Mital A.; March-Leuba, Jose A.

    2016-05-30

    The International Atomic Energy Agency (IAEA) has developed an On-Line Enrichment Monitor (OLEM) as one possible component in a new generation of safeguards measures for uranium enrichment plants. The OLEM measures 235U emissions from the UF6 gas flowing through a unit header pipe using NaI(Tl) spectrometers, and corrects for gas density changes using pressure and temperature sensors in order to determine the enrichment of the gas as a function of time. In parallel with the OLEM instrument development, a Virtual OLEM (VOLEM) software tool has been developed that is capable of producing synthetic gamma-ray, pressure, and temperature data representative of a wide range of enrichment plant operating conditions. VOLEM complements instrument development activities and allows the study of OLEM for scenarios that will be difficult or impossible to evaluate empirically. Uses of VOLEM include: investigation of hardware design options; inter-comparison of candidate gamma-ray spectral analysis and enrichment estimation algorithms; uncertainty budget analysis and performance prediction for typical and atypical operational scenarios; and testing of the OLEM data acquisition, analysis and reporting software. This paper describes the technical foundations of VOLEM and illustrates how it can be used. An overview of the nominal instrument design and deployment scenario for OLEM is provided, with emphasis on the key online-assay measurement challenge: accurately determining the portion of the total 235U signal that comes from a background that includes solid uranium deposits on the piping walls. Monte Carlo modeling tools, data analysis algorithms and uncertainty quantification methods are described. VOLEM is then used to quantitatively explore the uncertainty budgets and predicted instrument performance for a plausible range of typical plant operating parameters, and one set of candidate analysis algorithms. Additionally, a series of VOLEM case studies illustrates how an online

  6. Development of an enrichment method for endogenous phosphopeptide characterization in human serum.

    Science.gov (United States)

    La Barbera, Giorgia; Capriotti, Anna Laura; Cavaliere, Chiara; Ferraris, Francesca; Laus, Michele; Piovesana, Susy; Sparnacci, Katia; Laganà, Aldo

    2018-01-01

    The work describes the development of an enrichment method for the analysis of endogenous phosphopeptides in serum. Endogenous peptides can play significant biological roles, and some of them could be exploited as future biomarkers. In this context, blood is one of the most useful biofluids for screening, but a systematic investigation of the endogenous peptides, especially phosphorylated ones, is still lacking, mainly due to the lack of suitable analytical methods. Thus, in this paper, different phosphopeptide enrichment strategies were pursued, based either on metal oxide affinity chromatography (MOAC, in the form of commercial TiO 2 spin columns or magnetic graphitized carbon black-TiO 2 composite), or on immobilized metal ion affinity chromatography (IMAC, in the form of Ti 4+ -IMAC magnetic material or commercial Fe 3+ -IMAC spin columns). While MOAC strategies proved completely unsuccessful, probably due to interfering phospholipids displacing phosphopeptides, the IMAC materials performed very well. Different sample preparation strategies were tested, comprising direct dilution with the loading buffer, organic solvent precipitation, and lipid removal from the matrix, as well as the addition of phosphatase inhibitors during sample handling for maximized endogenous phosphopeptide enrichment. All data were acquired by a shotgun peptidomics approach, in which peptide samples were separated by reversed-phase nanoHPLC hyphenated with high-resolution tandem mass spectrometry. The devised method allowed the identification of 176 endogenous phosphopeptides in fresh serum added with inhibitors by the direct dilution protocol and the Ti 4+ -IMAC magnetic material enrichment, but good results could also be obtained from the commercial Fe 3+ -IMAC spin column adapted to the batch enrichment protocol.

  7. Avaliação da reprodutibilidade ultrassonográfica como método para medida da gordura abdominal e visceral Reproducibility of ultrasonography as a method to measure abdominal and visceral fat

    Directory of Open Access Journals (Sweden)

    Angélica Lemos Debs Diniz

    2009-12-01

    Full Text Available OBJETIVO: O objetivo deste estudo é avaliar a variabilidade interobservador do método ultrassonográfico para medida da gordura subcutânea, visceral e perirrenal por meio de técnica padronizada. MATERIAIS E MÉTODOS: Foram avaliados 50 pacientes entre novembro de 2006 e janeiro de 2007. A medida da espessura subcutânea foi realizada com transdutor linear de 7,5 MHz posicionado transversalmente a 1 cm acima da cicatriz umbilical. Para a gordura visceral foi utilizado transdutor de 3,5 MHz posicionado 1 cm acima da cicatriz umbilical, considerando-se a medida entre a face interna do músculo reto abdominal e a parede posterior da aorta na linha média do abdome. A gordura perirrenal foi medida no terço médio do rim direito, com transdutor posicionado na linha axilar média. RESULTADOS: A reprodutibilidade interobservador foi analisada por meio do teste t de Student, com significância de 95%. Não houve diferença significativa entre as médias das medidas das gorduras subcutânea, visceral e perirrenal, com p = 0,7141, 0,7286 e 0,6368, respectivamente. As médias encontradas, com seus respectivos desvios-padrão, foram: 2,64 ± 1,37 para a espessura subcutânea, 6,84 ± 2,38 para a espessura visceral e 4,89 ± 2,6 para a espessura perirrenal. CONCLUSÃO: A ultrassonografia apresentou boa reprodutibilidade interobservador para avaliação da gordura abdominal por meio das medidas das espessuras subcutânea, visceral e perirrenal.OBJECTIVE: The purpose of this study was to evaluate the interobserver variability of ultrasound in the measurement of subcutaneous, visceral and perirenal fat through standard technique. MATERIALS AND METHODS: From November 2006 to January 2007, 50 patients were evaluated. The subcutaneous fat thickness was measured with a 7.5 MHz linear transducer transversely positioned 1 cm above the umbilical scar. For the visceral fat, a 3.5 MHz transducer was also positioned 1 cm above the umbilical scar, considering the

  8. Medidas antidumping: instrumento del neoproteccionismo

    OpenAIRE

    Fernando Gutiérrez Solsona

    1994-01-01

    El objetivo fundamental de este trabajo se puede expresar fácilmente: demostrar que las medidas antidumping, que hoy en día son tan utilizadas por la Unión Europea (U.E.) y por los Estados Unidos (EE. UU.), tienen exclusivamente un fin proteccionista. Proteccionismo entendido en el sentido económico, esto es proteger a los sectores nacionales frente a la competencia extranjera, porque los primeros son menos eficientes que los segundos.

  9. Technologies and methods used for the detection, enrichment and characterization of cancer stem cells.

    Science.gov (United States)

    Williams, Anthony; Datar, Ram; Cote, Richard

    2010-01-01

    Cancer stem cells (CSCs) represent a subclass of tumour cells with the ability for self-renewal, production of differentiated progeny, prolonged survival, resistance to damaging therapeutic agents, and anchorage-independent survival, which together make this population effectively equipped to metastasize, invade and colonize secondary tissues in the face of therapeutic intervention. In recent years, investigators have increasingly focused on the characterization of CSCs to better understand the mechanisms that govern malignant disease progression in an effort to develop more effective, targeted therapeutic agents. The primary obstacle to the study of CSCs, however, is their rarity. Thus, the study of CSCs requires the use of sensitive and efficient technologies for their enrichment and detection. This review discusses technologies and methods that have been adapted and used to isolate and characterize CSCs to date, as well as new potential directions for the enhanced enrichment and detection of CSCs. While the technologies used for CSC enrichment and detection have been useful thus far for their characterization, each approach is not without limitations. Future studies of CSCs will depend on the enhanced sensitivity and specificity of currently available technologies, and the development of novel technologies for increased detection and enrichment of CSCs.

  10. Isotope enrichment

    International Nuclear Information System (INIS)

    Lydtin, H-J.; Wilden, R.J.; Severin, P.J.W.

    1978-01-01

    The isotope enrichment method described is based on the recognition that, owing to mass diffusion and thermal diffusion in the conversion of substances at a heated substrate while depositing an element or compound onto the substrate, enrichment of the element, or a compound of the element, with a lighter isotope will occur. The cycle is repeated for as many times as is necessary to obtain the degree of enrichment required

  11. Development of three-dimensional ENRICHED FREE MESH METHOD and its application to crack analysis

    International Nuclear Information System (INIS)

    Suzuki, Hayato; Matsubara, Hitoshi; Ezawa, Yoshitaka; Yagawa, Genki

    2010-01-01

    In this paper, we describe a method for three-dimensional high accurate analysis of a crack included in a large-scale structure. The Enriched Free Mesh Method (EFMM) is a method for improving the accuracy of the Free Mesh Method (FMM), which is a kind of meshless method. First, we developed an algorithm of the three-dimensional EFMM. The elastic problem was analyzed using the EFMM and we find that its accuracy compares advantageously with the FMM, and the number of CG iterations is smaller. Next, we developed a method for calculating the stress intensity factor by employing the EFMM. The structure with a crack was analyzed using the EFMM, and the stress intensity factor was calculated by the developed method. The analysis results were very well in agreement with reference solution. It was shown that the proposed method is very effective in the analysis of the crack included in a large-scale structure. (author)

  12. What Is the Best NGS Enrichment Method for the Molecular Diagnosis of Monogenic Diabetes and Obesity?

    Directory of Open Access Journals (Sweden)

    Julien Philippe

    Full Text Available Molecular diagnosis of monogenic diabetes and obesity is of paramount importance for both the patient and society, as it can result in personalized medicine associated with a better life and it eventually saves health care spending. Genetic clinical laboratories are currently switching from Sanger sequencing to next-generation sequencing (NGS approaches but choosing the optimal protocols is not easy. Here, we compared the sequencing coverage of 43 genes involved in monogenic forms of diabetes and obesity, and variant detection rates, resulting from four enrichment methods based on the sonication of DNA (Agilent SureSelect, RainDance technologies, or using enzymes for DNA fragmentation (Illumina Nextera, Agilent HaloPlex. We analyzed coding exons and untranslated regions of the 43 genes involved in monogenic diabetes and obesity. We found that none of the methods achieves yet full sequencing of the gene targets. Nonetheless, the RainDance, SureSelect and HaloPlex enrichment methods led to the best sequencing coverage of the targets; while the Nextera method resulted in the poorest sequencing coverage. Although the sequencing coverage was high, we unexpectedly found that the HaloPlex method missed 20% of variants detected by the three other methods and Nextera missed 10%. The question of which NGS technique for genetic diagnosis yields the highest diagnosis rate is frequently discussed in the literature and the response is still unclear. Here, we showed that the RainDance enrichment method as well as SureSelect, which are both based on the sonication of DNA, resulted in a good sequencing quality and variant detection, while the use of enzymes to fragment DNA (HaloPlex or Nextera might not be the best strategy to get an accurate sequencing.

  13. Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method.

    Science.gov (United States)

    Chen, Ming; Lin, Yong-Qing; Xie, Shuang-Lun; Wu, Hong-Fu; Wang, Jing-Feng

    2011-04-01

    Hanging drop (HD) culture is used to induce differentiation of embryonic stem cells (ESCs) into other cell types including cardiomyocytes. However, the factors affecting cardiac differentiation of ESCs with this method remain incompletely understood. We have investigated the effects of the starting number of ESCs in embryoid bodies (EBs) and the time of EB adherence to gelatin-coated plates on cardiac differentiation: cardiac differentiation was increased in the EBs by a larger number of ESCs and was decreased by plating EBs at day 4 or earlier. These two factors can thus be optimized to enrich the cardiac differentiation in ESCs using the HD method.

  14. Evaluation of enrichment by centrifugal separation: the future of the centrifugal-separation method

    International Nuclear Information System (INIS)

    Kanagawa, A.

    A gas centrifuge plant for uranium enrichment is considered from the point of view of economic competition with other methods. Characteristics of the method are presented including: energy efficiency, the cascade, the separation coefficient, the equilibrium separation process, and capability as centrifugal pump. The structure of an individual gas centrifuge separator is described including the rotating cylinder, mechanisms for gas injection and extraction, mechanisms for counter-streaming of gas, the axle holder mechanism, the gas sealing mechanism, and the driving mechanism. (U.S.)

  15. Enriched reproducing kernel particle method for fractional advection-diffusion equation

    Science.gov (United States)

    Ying, Yuping; Lian, Yanping; Tang, Shaoqiang; Liu, Wing Kam

    2018-06-01

    The reproducing kernel particle method (RKPM) has been efficiently applied to problems with large deformations, high gradients and high modal density. In this paper, it is extended to solve a nonlocal problem modeled by a fractional advection-diffusion equation (FADE), which exhibits a boundary layer with low regularity. We formulate this method on a moving least-square approach. Via the enrichment of fractional-order power functions to the traditional integer-order basis for RKPM, leading terms of the solution to the FADE can be exactly reproduced, which guarantees a good approximation to the boundary layer. Numerical tests are performed to verify the proposed approach.

  16. Desempenho do método de dissipação térmica na medida do fluxo de seiva em seringueira Performance of a thermal dissipation method for sap flow measurement in rubber tree

    Directory of Open Access Journals (Sweden)

    Juan S. Delgado-Rojas

    2006-12-01

    Full Text Available A medida da transpiração de árvores no campo é de difícil consecução. Nas duas últimas décadas, tem-se dado ênfase à medida de fluxo de seiva no caule como indicador da transpiração, principalmente se a escala de tempo usada é igual ou menor que um dia. Para essas medidas, são usados métodos térmicos com fornecimento de calor ao caule. O método de dissipação térmica (MDT é um deles e vem sendo bastante utilizado pelo seu princípio simples e pela boa sensibilidade às variações de fluxo de seiva. Entretanto, tem-se evidenciado a necessidade de cuidados no seu uso, sendo uma das causas de perda de precisão o efeito da carga radiante sobre a planta, geradora de gradiente térmico natural, que interfere nos resultados. Além disso, o desempenho do método em condições diferentes daquelas em que foi desenvolvido deve ser testado. Assim, o presente trabalho teve como objetivos analisar o desempenho do MDT em árvores de seringueira, considerando que essa espécie produz látex que poderia interferir sobre o bom funcionamento do sensor, e avaliar o efeito do gradiente térmico natural do caule sobre as estimativas. Os resultados demonstraram que essa técnica de medida pode ser utilizada nessa espécie e que, nas condições em que foram realizados os testes, não há interferência do gradiente térmico natural sobre as estimativas. Entretanto, para melhorar o grau de precisão, deve ser realizado melhor estudo de caraterização da área condutora da seiva em relação ao diâmetro do caule.The transpiration measurement of trees in field condition is very difficult. In the two last decades, some emphasis has been given in the study of stem sap flow as an indicator of plant transpiration. The methodology is particularly suitable for time scale less or equal than one day. One of the methods is the heat dissipation method (HDM, which has been used because of its simplicity and high sensitivity to the sap flow variations

  17. Application of a method to measure uranium enrichment without use of standards

    International Nuclear Information System (INIS)

    Saule, F.A.; Righetti, M.A.

    1998-01-01

    Full text: The determination of uranium enrichment in the many different stages present at a gaseous diffusion enrichment plant (diffusers, cisterns, deposits in pipes, drums with rests of process), or materials of deposit (plates of fuel elements not irradiated and recipients with uranium oxide), that have several geometries and physics properties of the containers, is very important for safeguards inspections. In this work is tested a non destructive analysis technique to determine the value of uranium enrichment of different samples with uranium materials without use of standards, to apply in safeguards inspections. It was used a hyper pure germanium detector with efficiency of 20% to obtain the gamma spectrum of the samples. In each spectrum, were used the net area values corresponding to four lines of U-235 (at 143, 163, 186 and 205 keV) and three lines of U-238 (258, 766 and 1001 keV); these values were analysed with two different methods. The comparison of the calculated and declared values showed a discrepancy of about 10%. (author) [es

  18. Methods for reducing lipid oxidation in fish-oil-enriched energy bars

    DEFF Research Database (Denmark)

    Nielsen, Nina Skall; Jacobsen, Charlotte

    2009-01-01

    P>Fish oil (FO) enrichment of foods is relevant owing to the beneficial effects of omega-3 polyunsaturated fatty acids on human health. However, the susceptibility of FO to oxidation necessitates careful control to avoid this oxidation. In this study, energy bars were successfully supplemented...... similar protection towards oxidation as packaging the energy bars in modified atmosphere. These protection methods were although not as efficient as addition of FO as micro-encapsulated powder. Addition of the metal chelator ethylene diamine tetra-acetic acid (EDTA) (100-2000 ppm) to emulsified FO...

  19. Modified micro-diffusion method for 15N-enriched soil solutions

    International Nuclear Information System (INIS)

    Aigner, M.

    2000-01-01

    The preparation of solutions for determination of 15 N/ 14 N isotope ratios is described, with special reference to dilute samples. A micro-diffusion method has been simplified to be more suitable for rapid isotope-ratio determination in soil solutions collected in tensionics. Ammonia expelled during micro-diffusion is captured on acidified filter discs fixed to the caps of gas-tight vials. The discs are transferred to tin capsules for shipment to the Soil Science Unit for 15 N-enrichment determination. (author)

  20. Effect of Dietary Fiber Enrichment and Different Cooking Methods on Quality of Chicken Nuggets.

    Science.gov (United States)

    Pathera, Ashok K; Riar, C S; Yadav, Sanjay; Sharma, D P

    2017-01-01

    The effect of dietary fiber enrichment (wheat bran) and cooking methods (oven, steam and microwave) on functional and physico-chemical properties of raw nuggets formulation as well as nutritional, color and textural properties of chicken nuggets were analyzed in this study. Among different cooking methods used for nuggets preparation, steam cooked nuggets had significantly ( p cooking yield (97.16%) and total dietary fiber content (4.32%) in comparison to oven and microwave cooked nuggets. The effect of cooking methods and wheat bran incorporation was also noticed on textural properties of the nuggets. Hardness, firmness and toughness values of oven and steam cooked nuggets were significantly ( p cooked nuggets. Among nuggets prepared by different cooking methods, cohesiveness of microwave cooked nuggets was found to be significantly ( p cooked nuggets had significantly ( p cooked nuggets were found to be better among all nuggets due to their higher cooking yield and dietary fiber content.

  1. Fast critical assembly safeguards: NDA methods for highly enriched uranium. Summary report, October 1978-September 1979

    International Nuclear Information System (INIS)

    Bellinger, F.O.; Winslow, G.H.

    1980-12-01

    Nondestructive assay (NDA) methods, principally passive gamma measurements and active neutron interrogation, have been studied for their safeguards effectiveness and programmatic impact as tools for making inventories of highly enriched uranium fast critical assembly fuel plates. It was concluded that no NDA method is the sole answer to the safeguards problem, that each of those emphasized here has its place in an integrated safeguards system, and that each has minimum facility impact. It was found that the 185-keV area, as determined with a NaI detector, was independent of highly-enriched uranium (HEU) plate irradiation history, though the random neutron driver methods used here did not permit accurate assay of irradiated plates. Containment procedures most effective for accurate assaying were considered, and a particular geometry is recommended for active interrogation by a random driver. A model, pertinent to that geometry, which relates the effects of multiplication and self-absorption, is described. Probabilities of failing to detect that plates are missing are examined

  2. THE INFLUENCE OF STORAGE CONDITIONS OF CANDIED FRUITS ENRICHED WITH VITAMIN C BY DIFFERENT METHODS ON ITS CONTENT

    Directory of Open Access Journals (Sweden)

    Agata Wypchoł

    2010-05-01

    Full Text Available The aim of this work was to study the effect of storage conditions of candied fruits enriched with vitamin C on its concentration in that product. The materials were candied fruits (black chokeberry and black currant enriched with vitamin C by two methods during their production. The final products were stored within 3 months at 8 and 20°C. On the basis of the results it was stated that enrichment method affected the concentration of the vitamin C in fruits measured during storage period at the above conditions. doi:10.5219/55

  3. MARCC (Matrix-Assisted Reader Chromatin Capture): an antibody-free method to enrich and analyze combinatorial nucleosome modifications

    Science.gov (United States)

    Su, Zhangli

    2016-01-01

    Combinatorial patterns of histone modifications are key indicators of different chromatin states. Most of the current approaches rely on the usage of antibodies to analyze combinatorial histone modifications. Here we detail an antibody-free method named MARCC (Matrix-Assisted Reader Chromatin Capture) to enrich combinatorial histone modifications. The combinatorial patterns are enriched on native nucleosomes extracted from cultured mammalian cells and prepared by micrococcal nuclease digestion. Such enrichment is achieved by recombinant chromatin-interacting protein modules, or so-called reader domains, which can bind in a combinatorial modification-dependent manner. The enriched chromatin can be quantified by western blotting or mass spectrometry for the co-existence of histone modifications, while the associated DNA content can be analyzed by qPCR or next-generation sequencing. Altogether, MARCC provides a reproducible, efficient and customizable solution to enrich and analyze combinatorial histone modifications. PMID:26131849

  4. Observability in electric power networks: identification critical measures methods; Observabilidade em redes de energia eletrica: metodos de identificacao de medidas criticas

    Energy Technology Data Exchange (ETDEWEB)

    London Junior, Joao Bosco Augusto

    1997-07-01

    One of the most important functions of the control and operation centers is to maintain service reliability in a electrical power system. In order to obtain a reliable operation of the power system, it is important to identify the critical measurements, and then to improve the measurement system using pseudo measurements. The goal of this work is to determine more efficient methods for critical measurement identification. A brief review of the some methods for observability analysis as well as two methodologies to identify critical measurements are presented. The first method has a combinatorial nature; the second one is supported by uni modular M matrix (incidence matrix of measurements for branches) and A matrix (incidence matrix of branch for nodes). The second method needs a combinatorial algorithm to be feasible, so that it becomes a slow method. Two new methods for critical measurements identification are presented in this work: the first one is based on the theory developed by Bretas (1996a), to analyse observability using graph paths; the second methods is supported y the Slutsker and Scudder (1987) theory, where identification is reached throughout the analysis of the jacobian matrix. (author)

  5. Determination of the response time of pressure transducers using the direct method; Determinacao do tempo de resposta de transdutores de pressao utilizando o metodo de medida direta

    Energy Technology Data Exchange (ETDEWEB)

    Perillo, S R.P.

    1994-12-31

    The available methods to determine the response time of nuclear safety related pressure transducers are discussed, with emphasis to the direct method. In order to perform the experiments, a Hydraulic Ramp Generator was built. The equipment produces ramp pressure transients simultaneously to a reference transducer and to the transducer under test. The time lag between the output of the two transducers, when they reach a predetermined setpoint, is measured as the time delay of the transducer under test. Some results using the direct method to determine the time delay of pressure transducers (1 E Class Conventional) are presented. (author). 18 refs, 35 figs, 12 tabs.

  6. Comparison of Listeria monocytogenes recoveries from spiked mung bean sprouts by the enrichment methods of three regulatory agencies.

    Science.gov (United States)

    Cauchon, Kaitlin E; Hitchins, Anthony D; Smiley, R Derike

    2017-09-01

    Three selective enrichment methods, the United States Food and Drug Administration's (FDA method), the United States Department of Agriculture Food Safety Inspection Service's (USDA method), and the EN ISO 11290-1 standard method, were assessed for their suitability for recovery of Listeria monocytogenes from spiked mung bean sprouts. Three parameters were evaluated; the enrichment L. monocytogenes population from singly-spiked sprouts, the enrichment L. monocytogenes population from doubly-spiked (L. monocytogenes and Listeria innocua) sprouts, and the population differential resulting from the enrichment of doubly-spiked sprouts. Considerable L. monocytogenes inter-strain variation was observed. The mean enrichment L. monocytogenes populations for singly-spiked sprouts were 6.1 ± 1.2, 4.9 ± 1.2, and 6.9 ± 2.3 log CFU/mL for the FDA, USDA, and EN ISO 11290-1 methods, respectively. The mean L. monocytogenes populations for doubly-spiked sprouts were 4.7 ± 1.1, 5.5 ± 1.3, and 4.6 ± 1.4 log CFU/mL for the FDA, USDA, and ISO 11290-1 enrichment methods, respectively. The corresponding mean population differentials were 2.8 ± 1.1, 3.3 ± 1.3, and 3.6 ± 1.4 Δlog CFU/mL for the same three enrichment methods, respectively. The presence of L. innocua and resident microorganisms on the sprouts negatively impacted final levels of L. monocytogenes with all three enrichment methods. Published by Elsevier Ltd.

  7. Enrichment method for trace amounts of rare earth elements using chemofiltration and XRF determination

    International Nuclear Information System (INIS)

    De Vito, I.E.; Olsina, R.A.; Masi, A.N.

    2000-01-01

    A preconcentration method for subsequent determination of rare earth elements (REE) by X-ray fluorescence (XRF) spectrometry was developed. The method is based on using (o-[3,6-disulfo-2-hydroxy-1-naphthylazo]-benzenearsonic acid) (Thorin) as a complexing agent which is retained on a polyamide membrane by a chemofiltration process. The pH dependence of the chemofiltration of these metal ions on the membrane and other variables, such as flow-rate, contact time, kinetic of complex formation, etc. were determined. The membrane containing the chemofiltrate formed a thin film, which eliminated the interelemental effects when measured by XRF. The detection limits were 23, 23 and 49 ng/mL for Sm(III), Eu(III) and Gd(III), respectively. High enrichment factors were obtained. The method was successfully applied to the preconcentration of Sm(III), Eu(III) and Gd(III) from different samples. (orig.)

  8. Non-destructive evaluation methods to improve quality control in low enrichment MTR fuel plate production

    International Nuclear Information System (INIS)

    Milne, J.M.; Lidington, B.; Hawker, B.M.

    1991-01-01

    This paper summarises some preliminary non-destructive measurements made recently at the Harwell Laboratory on a prototype low enrichment MTR fuel plate. The measurements were intended to indicate the potential of two different techniques for improving quality control in plate production. Pulse Video Thermography (PVT) is being considered as an alternative to ultrasound transmission measurements for the detection and sizing of lack of thermal bonding between the fuel and the clad layers, either to verify the indications from the established ultrasonic methods before destroying the plate or as a replacement method of inspection. High frequency pulse-echo ultrasonics is being considered for providing maps of clad layer thickness on each side of the plate. The measurements have indicated the potential for both methods, but more work is required, using a test plate containing controlled defects, to establish their capability. (orig.)

  9. Enrichment methods to detect bone marrow micrometastases in breast carcinoma patients: clinical relevance

    International Nuclear Information System (INIS)

    Choesmel, Valérie; Pierga, Jean-Yves; Nos, Claude; Vincent-Salomon, Anne; Sigal-Zafrani, Brigitte; Thiery, Jean-Paul; Blin, Nathalie

    2004-01-01

    Improving technologies for the detection and purification of bone marrow (BM) micrometastatic cells in breast cancer patients should lead to earlier prognosis of the risk of relapse and should make it possible to design more appropriate therapies. The technique used has to overcome the challenges resulting from the small number of target cells (one per million hematopoietic cells) and the heterogeneous expression of micrometastatic cell markers. In the present study, we have assessed the clinical relevance of current methods aimed at detecting rare disseminated carcinoma cells. BM aspirates from 32 carcinoma patients were screened for the presence of micrometastatic cells positive for epithelial cell adhesion molecule and positive for cytokeratins, using optimized immunodetection methods. A comparison with data obtained for 46 control BM aspirates and a correlation with the clinical status of patients were performed. We developed a sensitive and efficient immunomagnetic protocol for the enrichment of BM micrometastases. This method was used to divide 32 breast carcinoma patients into three categories according to their epithelial cell adhesion molecule status. These categories were highly correlated with the recently revised American Joint Committee on Cancer staging system for breast cancer, demonstrating the clinical relevance of this simple and reliable immunomagnetic technique. We also evaluated immunocytochemical detection of cytokeratin-positive cells and cytomorphological parameters. Immunocytochemistry-based methods for the detection of BM micrometastases did not provide any information about the clinical status of patients, but helped to refine the immunomagnetic data by confirming the presence of micrometastases in some cases. We also tested a new density gradient centrifugation system, able to enrich the tumor fraction of BM specimens by twofold to threefold as compared with standard Ficoll methods. These improved methods for the detection of

  10. Pros and cons of methylation-based enrichment methods for ancient DNA

    Science.gov (United States)

    Seguin-Orlando, Andaine; Gamba, Cristina; Sarkissian, Clio Der; Ermini, Luca; Louvel, Guillaume; Boulygina, Eugenia; Sokolov, Alexey; Nedoluzhko, Artem; Lorenzen, Eline D.; Lopez, Patricio; McDonald, H. Gregory; Scott, Eric; Tikhonov, Alexei; Stafford,, Thomas W.; Alfarhan, Ahmed H.; Alquraishi, Saleh A.; Al-Rasheid, Khaled A. S.; Shapiro, Beth; Willerslev, Eske; Prokhortchouk, Egor; Orlando, Ludovic

    2015-01-01

    The recent discovery that DNA methylation survives in fossil material provides an opportunity for novel molecular approaches in palaeogenomics. Here, we apply to ancient DNA extracts the probe-independent Methylated Binding Domains (MBD)-based enrichment method, which targets DNA molecules containing methylated CpGs. Using remains of a Palaeo-Eskimo Saqqaq individual, woolly mammoths, polar bears and two equine species, we confirm that DNA methylation survives in a variety of tissues, environmental contexts and over a large temporal range (4,000 to over 45,000 years before present). MBD enrichment, however, appears principally biased towards the recovery of CpG-rich and long DNA templates and is limited by the fast post-mortem cytosine deamination rates of methylated epialleles. This method, thus, appears only appropriate for the analysis of ancient methylomes from very well preserved samples, where both DNA fragmentation and deamination have been limited. This work represents an essential step toward the characterization of ancient methylation signatures, which will help understanding the role of epigenetic changes in past environmental and cultural transitions. PMID:26134828

  11. A digital method for period measurements in a nuclear reactor; Um metodo digital para medidas de periodo em um reator nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Mundim, Sergio Gorretta

    1971-02-15

    The present paper begins by giving a theoretical treatment for the nuclear reactor period. The conventional method of measuring the period is analysed and some previously developed digital methods are described. The paper criticises the latter, pointing out some deficiencies which the proposed process is able to eliminate. All errors connected with this process are also analysed. The paper presents suitable solutions to reduce them to a minimum. The total error is found to he less than the error presented by the other methods described. A digital period meter is designed with memory resources and an automatic scaler changer. Integrated circuits specifications are used in it. Real time experiments with nuclear reactors were made in order to check te validity of the method. The data acquired were applied to a simulated digital period meter implemented in a general purpose computer. The nuclear part of the work was developed at the 'Comissao Nacional de Energia Nuclear' and the simulation work was dane at the 'Departamento de Calculo Cientifico' of COPPE, which also advised the author in the completion of this thesis. (author)

  12. A digital method for period measurements in a nuclear reactor; Um metodo digital para medidas de periodo em um reator nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Mundim, Sergio Gorretta

    1971-02-15

    The present paper begins by giving a theoretical treatment for the nuclear reactor period. The conventional method of measuring the period is analysed and some previously developed digital methods are described. The paper criticises the latter, pointing out some deficiencies which the proposed process is able to eliminate. All errors connected with this process are also analysed. The paper presents suitable solutions to reduce them to a minimum. The total error is found to he less than the error presented by the other methods described. A digital period meter is designed with memory resources and an automatic scaler changer. Integrated circuits specifications are used in it. Real time experiments with nuclear reactors were made in order to check te validity of the method. The data acquired were applied to a simulated digital period meter implemented in a general purpose computer. The nuclear part of the work was developed at the 'Comissao Nacional de Energia Nuclear' and the simulation work was dane at the 'Departamento de Calculo Cientifico' of COPPE, which also advised the author in the completion of this thesis. (author)

  13. Bubble-Enriched Least-Squares Finite Element Method for Transient Advective Transport

    Directory of Open Access Journals (Sweden)

    Rajeev Kumar

    2008-01-01

    Full Text Available The least-squares finite element method (LSFEM has received increasing attention in recent years due to advantages over the Galerkin finite element method (GFEM. The method leads to a minimization problem in the L2-norm and thus results in a symmetric and positive definite matrix, even for first-order differential equations. In addition, the method contains an implicit streamline upwinding mechanism that prevents the appearance of oscillations that are characteristic of the Galerkin method. Thus, the least-squares approach does not require explicit stabilization and the associated stabilization parameters required by the Galerkin method. A new approach, the bubble enriched least-squares finite element method (BELSFEM, is presented and compared with the classical LSFEM. The BELSFEM requires a space-time element formulation and employs bubble functions in space and time to increase the accuracy of the finite element solution without degrading computational performance. We apply the BELSFEM and classical least-squares finite element methods to benchmark problems for 1D and 2D linear transport. The accuracy and performance are compared.

  14. Hemoglobin measured by Hemocue and a reference method in venous and capillary blood: a validation study Hemoglobina medida por Hemocue y por un método de referencia en sangre venosa y capilar: estudio de validación

    Directory of Open Access Journals (Sweden)

    Lynnette Neufeld

    2002-06-01

    Full Text Available Objective. To assess the comparability of hemoglobin concentration (Hb in venous and capillary blood measured by Hemocue and an automated spectrophotometer (Celldyn and to document the influence of type of blood (capillary or venous and analysis method on anemia prevalence estimates. Material and Methods. Between February and May 2000, capillary and venous samples were collected from 72 adults and children at Hospital del Niño Morelense (Morelos State Children's Hospital in Cuernavaca, Morelos, Mexico, and assessed for Hb using the Hemocue and Celldyn methods. Estimated Hb levels were compared using the concordance correlation coefficient and Student's t test for paired data. The sensitivity and specificity for anemia diagnosis were estimated and compared between type of blood and method of assessment. Results. Capillary blood had higher Hb (+0.5g/dl than venous blood in adults and children, as did samples assessed by Celldyn compared to Hemocue (+0.3g/dl. Specificity to detect anemia was adequate (>0.90 but sensitivity was low for capillary blood assessed by Hemocue (Objetivo. Evaluar la comparabilidad de la concentración de hemoglobina (Hb en sangre venosa y capilar medida por Hemocue y por espectrofotómetro automatizado (Celldyn, así como documentar la influencia del tipo de sangre (capilar o venosa y del método de análisis sobre la prevalencia de anemia. Material y métodos. De febrero a mayo de 2000, se recolectaron muestras de sangre capilar y venosa en 72 adultos y niños en el Hospital del Niño Morelense, Cuernavaca, Morelos, México. Se determinaron los niveles de Hb con los métodos Hemocue y Celldyn. Las cifras de Hb estimadas se compararon con el coeficiente de concordancia y la prueba pareada de t de Student. También se comparó la sensibilidad y especificidad para el diagnóstico de anemia, utilizando sangre de los dos tipos y métodos de análisis. Resultados. La Hb fue mayor en sangre capilar comparada con sangre venosa

  15. A spectrometrical method to measure the deuterium content in 2H-enriched water

    International Nuclear Information System (INIS)

    Dumke, I.

    1980-04-01

    A test method and spectrometer has been developed for emission-spectrometrical measurement of the deuterium content in water enriched with deuterium. The water sample is melted into a previously evacuated glas tube and a gas discharge is excited in vapour over the cooled sample to adjust to a low vapour pressure with high frequency. The intensities of the H(α) and D(α) lines appearing in the spectrum determine the D-content. Both lines were resolved by a Fabry-Perot interferometer and geometrically separated fed to two photodetectors. The remaining spectrum is filtered off. Following electronic calculation of the signals, the measured value is indicated which has to be corrected by a standard curve. The relative measuring accuracy is about +-1% for enrichments of over 1% D and less than +-5% in the region of 0.3-1% D. The detection limit is about 0.03% D (sample amount: 50 μl, average of 5 samples). (orig./HP) [de

  16. Benchmarking methods and data sets for ligand enrichment assessment in virtual screening.

    Science.gov (United States)

    Xia, Jie; Tilahun, Ermias Lemma; Reid, Terry-Elinor; Zhang, Liangren; Wang, Xiang Simon

    2015-01-01

    Retrospective small-scale virtual screening (VS) based on benchmarking data sets has been widely used to estimate ligand enrichments of VS approaches in the prospective (i.e. real-world) efforts. However, the intrinsic differences of benchmarking sets to the real screening chemical libraries can cause biased assessment. Herein, we summarize the history of benchmarking methods as well as data sets and highlight three main types of biases found in benchmarking sets, i.e. "analogue bias", "artificial enrichment" and "false negative". In addition, we introduce our recent algorithm to build maximum-unbiased benchmarking sets applicable to both ligand-based and structure-based VS approaches, and its implementations to three important human histone deacetylases (HDACs) isoforms, i.e. HDAC1, HDAC6 and HDAC8. The leave-one-out cross-validation (LOO CV) demonstrates that the benchmarking sets built by our algorithm are maximum-unbiased as measured by property matching, ROC curves and AUCs. Copyright © 2014 Elsevier Inc. All rights reserved.

  17. Multi-layer solid-phase extraction and evaporation-enrichment methods for polar organic chemicals from aqueous matrices.

    Science.gov (United States)

    Köke, Niklas; Zahn, Daniel; Knepper, Thomas P; Frömel, Tobias

    2018-03-01

    Analysis of polar organic chemicals in the aquatic environment is exacerbated by the lack of suitable and widely applicable enrichment methods. In this work, we assessed the suitability of a novel combination of well-known solid-phase extraction (SPE) materials in one cartridge as well as an evaporation method and for the enrichment of 26 polar model substances (predominantly log D evaporation method were investigated for the recovery and matrix effects of the model substances and analyzed with hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). In total, 65% of the model substances were amenable (> 10% recovery) to the mlSPE method with a mean recovery of 76% while 73% of the model substances were enriched with the evaporation method achieving a mean recovery of 78%. Target and non-target screening comparison of both methods with a frequently used reversed-phase SPE method utilizing "hydrophilic and lipophilic balanced" (HLB) material was performed. Target analysis showed that the mlSPE and evaporation method have pronounced advantages over the HLB method since the HLB material retained only 30% of the model substances. Non-target screening of a ground water sample with the investigated enrichment methods showed that the median retention time of all detected features on a HILIC system decreased in the order mlSPE (3641 features, median t R 9.7 min), evaporation (1391, 9.3 min), HLB (4414, 7.2 min), indicating a higher potential of the described methods to enrich polar analytes from water compared with HLB-SPE. Graphical abstract Schematic of the method evaluation (recovery and matrix effects) and method comparison (target and non-target analysis) of the two investigated enrichment methods for very polar chemicals in aqueousmatrices.

  18. Método para medida do comprimento da cóclea por meio de ressonância magnética A method for measuring the length of the coclea through magnetic resonance imaging

    Directory of Open Access Journals (Sweden)

    Fernando Pochini Sobrinho

    2009-04-01

    Full Text Available Sabe-se que a deficiência auditiva afeta boa parte da população. Nos casos em que a surdez é profunda e bilateral, podem ocorrer problemas na aquisição de fala nas crianças, bem como na comunicação e socialização do indivíduo. O implante coclear vem sendo utilizado como opção terapêutica para esses casos. Na atualidade a Ressonância magnética da orelha interna é exame obrigatório na avaliação pré-operatória destes pacientes. No dia-a-dia, nos perguntamos se a ressonância magnética pode fornecer dados não só qualitativos como também quantitativos, com medidas lineares reais na cóclea reconstruída em imagens em três dimensões. OBJETIVO: O objetivo deste estudo é propor uma técnica para obtenção de medidas do comprimento da cóclea em imagens de ressonância magnética obtidos de ossos temporais de cadáveres. MATERIAL E MÉTODO: Foram avaliadas imagens de reconstrução em três dimensões da cóclea de seis cadáveres. Por meio de sobreposição de réguas digitalizadas sobre estas imagens foi possível medir o comprimento coclear. RESULTADO: Estas medidas variaram de 17 a 26,5 milímetros. CONCLUSÃO: Conclui-se que foi possível medir o comprimento da cóclea em imagens em três dimensões de ressonância magnética pelo método proposto.We know that hearing impairment affects a large part of the population. In cases of profound and bilateral hearing loss, children may have problems in speech development, as well as communication and socialization. Cochlear implants have been used as a treatment option in these cases. Today, inner ear MRI is a mandatory test in the preoperative evaluation of these individuals. In our daily routines, we wonder whether MRI can provide not only qualitative, but also quantitative data, with real cochlear linear values built from three dimension images. AIMS: The aim of the present investigation is to propose a method to obtain MRI cochlear length measures from the temporal bones

  19. Medida de espessura de filmes finos com interferometro de haidinger

    OpenAIRE

    Elisabeth Andreoli de Oliveira

    1987-01-01

    Resumo: Apresentamos um método não destrutivo para medida de espessura de filmes finos transparentes depositados em substratos transparentes, usando um interferômetro de Haidinger. A partir das frações de interferência, medidas para vários comprimentos de onda, e do índice de refração para um único comprimento de onda, determinamos a espessura do filme e sua dispersão cromática, através de um procedimento analítico-numérico Abstract: We introduce a non-destructive method for the thickness ...

  20. Method for semi-automated microscopy of filtration-enriched circulating tumor cells.

    Science.gov (United States)

    Pailler, Emma; Oulhen, Marianne; Billiot, Fanny; Galland, Alexandre; Auger, Nathalie; Faugeroux, Vincent; Laplace-Builhé, Corinne; Besse, Benjamin; Loriot, Yohann; Ngo-Camus, Maud; Hemanda, Merouan; Lindsay, Colin R; Soria, Jean-Charles; Vielh, Philippe; Farace, Françoise

    2016-07-14

    Circulating tumor cell (CTC)-filtration methods capture high numbers of CTCs in non-small-cell lung cancer (NSCLC) and metastatic prostate cancer (mPCa) patients, and hold promise as a non-invasive technique for treatment selection and disease monitoring. However filters have drawbacks that make the automation of microscopy challenging. We report the semi-automated microscopy method we developed to analyze filtration-enriched CTCs from NSCLC and mPCa patients. Spiked cell lines in normal blood and CTCs were enriched by ISET (isolation by size of epithelial tumor cells). Fluorescent staining was carried out using epithelial (pan-cytokeratins, EpCAM), mesenchymal (vimentin, N-cadherin), leukocyte (CD45) markers and DAPI. Cytomorphological staining was carried out with Mayer-Hemalun or Diff-Quik. ALK-, ROS1-, ERG-rearrangement were detected by filter-adapted-FISH (FA-FISH). Microscopy was carried out using an Ariol scanner. Two combined assays were developed. The first assay sequentially combined four-color fluorescent staining, scanning, automated selection of CD45(-) cells, cytomorphological staining, then scanning and analysis of CD45(-) cell phenotypical and cytomorphological characteristics. CD45(-) cell selection was based on DAPI and CD45 intensity, and a nuclear area >55 μm(2). The second assay sequentially combined fluorescent staining, automated selection of CD45(-) cells, FISH scanning on CD45(-) cells, then analysis of CD45(-) cell FISH signals. Specific scanning parameters were developed to deal with the uneven surface of filters and CTC characteristics. Thirty z-stacks spaced 0.6 μm apart were defined as the optimal setting, scanning 82 %, 91 %, and 95 % of CTCs in ALK-, ROS1-, and ERG-rearranged patients respectively. A multi-exposure protocol consisting of three separate exposure times for green and red fluorochromes was optimized to analyze the intensity, size and thickness of FISH signals. The semi-automated microscopy method reported here

  1. Method for semi-automated microscopy of filtration-enriched circulating tumor cells

    International Nuclear Information System (INIS)

    Pailler, Emma; Oulhen, Marianne; Billiot, Fanny; Galland, Alexandre; Auger, Nathalie; Faugeroux, Vincent; Laplace-Builhé, Corinne; Besse, Benjamin; Loriot, Yohann; Ngo-Camus, Maud; Hemanda, Merouan; Lindsay, Colin R.; Soria, Jean-Charles; Vielh, Philippe; Farace, Françoise

    2016-01-01

    Circulating tumor cell (CTC)-filtration methods capture high numbers of CTCs in non-small-cell lung cancer (NSCLC) and metastatic prostate cancer (mPCa) patients, and hold promise as a non-invasive technique for treatment selection and disease monitoring. However filters have drawbacks that make the automation of microscopy challenging. We report the semi-automated microscopy method we developed to analyze filtration-enriched CTCs from NSCLC and mPCa patients. Spiked cell lines in normal blood and CTCs were enriched by ISET (isolation by size of epithelial tumor cells). Fluorescent staining was carried out using epithelial (pan-cytokeratins, EpCAM), mesenchymal (vimentin, N-cadherin), leukocyte (CD45) markers and DAPI. Cytomorphological staining was carried out with Mayer-Hemalun or Diff-Quik. ALK-, ROS1-, ERG-rearrangement were detected by filter-adapted-FISH (FA-FISH). Microscopy was carried out using an Ariol scanner. Two combined assays were developed. The first assay sequentially combined four-color fluorescent staining, scanning, automated selection of CD45 − cells, cytomorphological staining, then scanning and analysis of CD45 − cell phenotypical and cytomorphological characteristics. CD45 − cell selection was based on DAPI and CD45 intensity, and a nuclear area >55 μm 2 . The second assay sequentially combined fluorescent staining, automated selection of CD45 − cells, FISH scanning on CD45 − cells, then analysis of CD45 − cell FISH signals. Specific scanning parameters were developed to deal with the uneven surface of filters and CTC characteristics. Thirty z-stacks spaced 0.6 μm apart were defined as the optimal setting, scanning 82 %, 91 %, and 95 % of CTCs in ALK-, ROS1-, and ERG-rearranged patients respectively. A multi-exposure protocol consisting of three separate exposure times for green and red fluorochromes was optimized to analyze the intensity, size and thickness of FISH signals. The semi-automated microscopy method reported here

  2. Enrichment of trace cadmium by soybean protein for the analysis by atomic absorption method

    International Nuclear Information System (INIS)

    Musha, Soichiro; Takahashi, Yoshihisa.

    1975-01-01

    A method for enrichment of the ppb level of cadmium in water by using the coagulation of soybean protein by adding acids or its complex-forming character with heavy metal ions was investigated. After adding fixed amounts of soybean milk and 2% sodium diethyldithiocarbamate(DDTC) aqueous solution and a suitable amount of delta-gluconic lactone (delta-GL) to a sample solution, the mixture was heated to boiling in order to coagulate the protein. The coagulum(soybean curd) was separated from the suspension by centrifugation and burned to ashes with a low temperature plasma asher. Then the cadmium enriched in it was determined by atomic absorption spectrometry. Various factors such as the pH of the sample solution, the amounts of soybean milk and the collection additives, and the concentration of NaCl in the sample solution on the recovery of cadmium were examined systematically. The best recovery was obtained under the following conditions: To a certain amount of sample solution were added 30 ml of 6.34% soybean milk and a 5 ml of 2% DDTC solution, and its pH was adjusted to 5.50--5.80 by adding the suitable amounts of delta-GL (0.10 g/ml, (0.40--0.80)ml). NaCl in the sample solution tended to decrease the recovery, especially at the concentration of around 10% of NaCl solution. Under the optimum conditions, the recovery of cadmium was about 98%. The proposed method was applied to the determination of cadmium at the ppb level in sample solutions such as water, 3% NaCl solution and artifical sea water. This method was also applied to the determination of cadmium in common and industrial salts. (auth.)

  3. Confiabilidade e validade das medidas da cifose torácica através do método flexicurva Reliability and validity of thoracic kyphosis measurements using flexicurve method

    Directory of Open Access Journals (Sweden)

    FA Teixeira

    2007-06-01

    Full Text Available CONTEXTUALIZAÇÃO: A hipercifose torácica é uma das alterações posturais mais comuns, sendo definida como um aumento da curvatura torácica no plano sagital da coluna vertebral. Uma cifose normal varia de 20 a 50º quando avaliada pelo método radiográfico de Cobb. O método radiográfico é o mais popular para mensuração da cifose, mas em função de ser um método caro e que expõe os sujeitos a radiação, não é o método mais adequado para acompanhamento periódico do paciente. Exames clínicos de rotina, como a avaliação fisioterapêutica da cifose torácica, devem ser válidos, confiáveis, sensíveis, práticos e baratos. OBJETIVOS: Verificar a validade concorrente e as confiabilidades intra e interavaliador das medidas de cifose torácica utilizando o Método Flexicurva. MÉTODOS: Estudo transversal em que a cifose torácica de 56 pessoas foi avaliada em radiografias sagitais da coluna torácica pelo método de Cobb e pelo Método Flexicurva por dois avaliadores. RESULTADOS: O coeficiente de correlação intraclasse (ICC entre as medidas pelo Cobb e pelo método Flexicurva foi de 0,906. A sensibilidade foi de 85%, a especificidade de 97% para diagnosticar a hipercifose torácica. CONCLUSÃO: O Método Flexicurva demonstra ser um parâmetro clínico quantitativo excelente para mensuração da curvatura da cifose torácica.BACKGROUND: Thoracic hyperkyphosis is one of the most common postural abnormalities. It is defined as increased thoracic curvature in the sagittal plan of the vertebral column. Normal kyphosis may range from 20º to 50º according to Cobb's radiographic method. The radiographic method is the most popular kyphosis measuring method, but because it is an expensive method and it exposes the individual to radiation, it is not the most appropriate method for periodic patient follow-up. Routine clinical examinations such as physiotherapeutic evaluation of thoracic kyphosis need to be valid, reliable, sensitive

  4. Application of protein purification methods for the enrichment of a cytotoxin from Campylobacter jejuni

    Directory of Open Access Journals (Sweden)

    Gatsos Xenia

    2012-12-01

    Full Text Available Abstract Background Campylobater jejuni, a major foodborne diarrhoeal pathogen is reported to produce a number of cytotoxins of which only a cytolethal distending toxin (CDT has been characterised so far. One or more additional cytotoxins other than CDT, including a Chinese hamster ovary (CHO cell active, Vero cell inactive cytotoxin, may mediate inflammatory diarrhoea. Our objective was to develop a method to enrich and thus partially characterise this cytotoxin, as a pathway to the eventual identification and characterisation of the toxin. Results A number of biochemical methods including cation- and anion-exchange chromatography were evaluated to enrich the cytotoxin from a cell lysate of a known cytotoxin-producing C. jejuni, C31. The cytotoxin in crude lysate was initially prepared by size-exclusion desalting and then subjected to high pressure liquid chromatography (HPLC ion-exchange fractionation. One pooled fraction (pool B was cytotoxic for CHO cells equivalent to crude toxin (tissue culture infectivity dose 50 [TCID50] of 1–2 μg/ml. The proteins of pool B were identified by mass spectrometry (MS after separation by SDS-PAGE and trypsin digestion. Also, pool B was directly digested with trypsin and then subjected to liquid chromatography tandem mass spectrometry (LCMS analysis for identification of lesser abundant proteins in the fraction. A total of 41 proteins were found in the fraction, which included enzymes involved in metabolic and transport functions. Eighteen non-cytoplasmic proteins including 2 major antigenic peptide proteins (PEB2 and PEB3 and 3 proteins of unknown function were also identified in the screen. Cytotoxicity in pool B was trypsin-sensitive indicating its protein nature. The cytotoxic activity was heat-stable to 50°C, and partially inactivated at 60-70°C. The pool B fraction also induced fluid accumulation in the adult rabbit ileal loop assay with cytotoxicity for mucosa confirming the presence of the

  5. Determination of the Avogadro constant by the XRCD method using a 28Si-enriched sphere

    Science.gov (United States)

    Kuramoto, Naoki; Mizushima, Shigeki; Zhang, Lulu; Fujita, Kazuaki; Azuma, Yasushi; Kurokawa, Akira; Okubo, Sho; Inaba, Hajime; Fujii, Kenichi

    2017-10-01

    To determine the Avogadro constant N A by the x-ray crystal density method, the density of a 28Si-enriched crystal was determined by absolute measurements of the mass and volume of a 1 kg sphere manufactured from the crystal. The mass and volume were determined by an optical interferometer and a vacuum mass comparator, respectively. The sphere surface was characterized by x-ray photoelectron spectroscopy and spectroscopic ellipsometry to derive the mass and volume of the Si core of the sphere excluding the surface layers. From the mass and volume, the density of the Si core was determined with a relative standard uncertainty of 2.3  ×  10-8. By combining the Si core density with the lattice constant and the molar mass of the sphere reported by the International Avogadro Coordination (IAC) project in 2015, a new value of 6.022 140 84(15)  ×  1023 mol-1 was obtained for N A with a relative standard uncertainty of 2.4  ×  10-8. To make the N A value determined in this work usable for a future adjustment of the fundamental constants by the CODATA Task Group on Fundamental Constants, the correlation of the new N A value with the N A values determined in our previous works was examined. The correlation coefficients with the values of N A determined by IAC in 2011 and 2015 were estimated to be 0.07 and 0.28, respectively. The correlation of the new N A value with the N A value determined by IAC in 2017 using a different 28Si-enriched crystal was also examined, and the correlation coefficient was estimated to be 0.21.

  6. Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Hongyan [Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Shinan District, Qingdao 266071, Shandong (China); College of Marine Science and Engineering, University of Science and Technology, Tianjin 300457 (China); Graduate School, Chinese Academy of Sciences, Beijing 100039 (China); Wang, Guangce [Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Shinan District, Qingdao 266071, Shandong (China); College of Marine Science and Engineering, University of Science and Technology, Tianjin 300457 (China); Zhu, Daling; Pan, Guanghua [College of Marine Science and Engineering, University of Science and Technology, Tianjin 300457 (China)

    2009-12-15

    To determine the effects of pretreatment on hydrogen production and the hydrogen-producing microbial community, we treated the sludge from the intertidal zone of a bathing beach in Tianjin with four different pretreatment methods, including acid treatment, heat-shock, base treatment as well as freezing and thawing. The results showed that acid pretreatment significantly promoted the hydrogen production by sludge and provided the highest efficiency of hydrogen production among the four methods. The efficiency of the hydrogen production of the acid-pretreated sludge was 0.86 {+-} 0.07 mol H{sub 2}/mol glucose (mean {+-} S.E.), whereas that of the sludge treated with heat-shock, freezing and thawing, base method and control was 0.41 {+-} 0.03 mol H{sub 2}/mol glucose, 0.17 {+-} 0.01 mol H{sub 2}/mol glucose, 0.11 {+-} 0.01 mol H{sub 2}/mol glucose and 0.20 {+-} 0.04 mol H{sub 2}/mol glucose, respectively. The result of denaturing gradient gel electrophoresis (DGGE) showed that pretreatment methods altered the composition of the microbial community that accounts for hydrogen production. Acid and heat pretreatments were favorable to enrich the dominant hydrogen-producing bacterium, i.e. Clostridium sp., Enterococcus sp. and Bacillus sp. However, besides hydrogen-producing bacteria, much non-hydrogen-producing Lactobacillus sp. was also found in the sludge pretreated with base, freezing and thawing methods. Therefore, based on our results, we concluded that, among the four pretreatment methods using acid, heat-shock, base or freezing and thawing, acid pretreatment was the most effective method for promoting hydrogen production of microbial community. (author)

  7. Determining method and device for enrichment distribution inside of fuel assembly

    International Nuclear Information System (INIS)

    Hirano, Yasushi; Hida, Kazuki; Sakurada, Koichi.

    1997-01-01

    An enrichment degree at an initial burning stage of each of fuel rods of a BWR type reactor assembly is divided into groups. The enrichment degree at the initial burning stage of each of the groups is inputted, and the burning period from the loading to the taking out is divided into a plurality of burning steps. Nuclear characteristics of fuel assemblies such as the power of fuel rods, R-factor and infinite multiplication factor in each of the burning steps are estimated. The enrichment degree of the group of enrichment degree at the initial burning stage and the estimated power of fuel rods in a reactor operation state during the burning step are stored in the memory. A sensitivity coefficient showing the amount of change of the power of fuel rods in the burning step relative to the change of the enrichment degree of the group of enrichment degree is evaluated. A weighing function in the burning step is inputted. The maximum value of the product of the weighing function and the power of fuel rods throughout the entire burning steps is determined as an aimed function. Optimization calculation is conducted for determining the enrichment degree of the group so as to minimize the aimed function thereby determining the distribution of the enrichment degree. (N.H.)

  8. Enrichment of Female Germline Stem Cells from Mouse Ovaries Using the Differential Adhesion Method

    Directory of Open Access Journals (Sweden)

    Meng Wu

    2018-04-01

    Full Text Available Background/Aims: The isolation and establishment of female germline stem cells (FGSCs is controversial because of questions regarding the reliability and stability of the isolation method using antibody targeting mouse vasa homologue (MVH, and the molecular mechanism of FGSCs self-renewal remains unclear. Thus, there needs to be a simple and reliable method for sorting FGSCs to study them. Methods: We applied the differential adhesion method to enrich FGSCs (DA-FGSCs from mouse ovaries. Through four rounds of purification and 7-9 subsequent passages, DA-FGSC lines were established. In addition, we assessed the role of the phosphoinositide-3 kinase (PI3K-AKT pathway in regulating FGSC self-renewal. Results: The obtained DA-FGSCs spontaneously differentiated into oocyte-like cells in vitro and formed functional eggs in vivo that were fertilized and produced healthy offspring. AKT was rapidly phosphorylated when the proliferation rate of FGSCs increased after 10 passages, and the addition of a chemical PI3K inhibitor prevented FGSCs self-renewal. Furthermore, over-expression of AKT-induced proliferation and differentiation of FGSCs, c-Myc, Oct-4 and Gdf-9 levels were increased. Conclusions: The differential adhesion method provides a more feasible approach and is an easier procedure to establish FGSC lines than traditional methods. The AKT pathway plays an important role in regulation of the proliferation and maintenance of FGSCs. These findings could help promote stem cell studies and provide a better understanding of causes of ovarian infertility, thereby providing potential treatments for infertility.

  9. Enrichment of Female Germline Stem Cells from Mouse Ovaries Using the Differential Adhesion Method.

    Science.gov (United States)

    Wu, Meng; Xiong, Jiaqiang; Ma, Lingwei; Lu, Zhiyong; Qin, Xian; Luo, Aiyue; Zhang, Jinjin; Xie, Huan; Shen, Wei; Wang, Shixuan

    2018-04-28

    The isolation and establishment of female germline stem cells (FGSCs) is controversial because of questions regarding the reliability and stability of the isolation method using antibody targeting mouse vasa homologue (MVH), and the molecular mechanism of FGSCs self-renewal remains unclear. Thus, there needs to be a simple and reliable method for sorting FGSCs to study them. We applied the differential adhesion method to enrich FGSCs (DA-FGSCs) from mouse ovaries. Through four rounds of purification and 7-9 subsequent passages, DA-FGSC lines were established. In addition, we assessed the role of the phosphoinositide-3 kinase (PI3K)-AKT pathway in regulating FGSC self-renewal. The obtained DA-FGSCs spontaneously differentiated into oocyte-like cells in vitro and formed functional eggs in vivo that were fertilized and produced healthy offspring. AKT was rapidly phosphorylated when the proliferation rate of FGSCs increased after 10 passages, and the addition of a chemical PI3K inhibitor prevented FGSCs self-renewal. Furthermore, over-expression of AKT-induced proliferation and differentiation of FGSCs, c-Myc, Oct-4 and Gdf-9 levels were increased. The differential adhesion method provides a more feasible approach and is an easier procedure to establish FGSC lines than traditional methods. The AKT pathway plays an important role in regulation of the proliferation and maintenance of FGSCs. These findings could help promote stem cell studies and provide a better understanding of causes of ovarian infertility, thereby providing potential treatments for infertility. © 2018 The Author(s). Published by S. Karger AG, Basel.

  10. Pruned-enriched Rosenbluth method: Simulations of θ polymers of chain length up to 1000000

    International Nuclear Information System (INIS)

    Grassberger, P.

    1997-01-01

    We present an algorithm for simulating flexible chain polymers. It combines the Rosenbluth-Rosenbluth method with recursive enrichment. Although it can be applied also in more general situations, it is most efficient for three-dimensional θ polymers on the simple-cubic lattice. There it allows high statistics simulations of chains of length up to N=10 6 . For storage reasons, this is feasable only for polymers in a finite volume. For free θ polymers in infinite volume, we present very high statistics runs with N=10000. These simulations fully agree with previous simulations made by Hegger and Grassberger [J. Chem. Phys. 102, 6681 (1995)] with a similar but less efficient algorithm, showing that logarithmic corrections to mean field behavior are much stronger than predicted by field theory. But the finite volume simulations show that the density inside a collapsed globule scales with the distance from the θ point as predicted by mean field theory, in contrast to claims in the work mentioned above. In addition to the simple-cubic lattice, we also studied two versions of the bond fluctuation model, but with much shorter chains. Finally, we show that our method can be applied also to off-lattice models, and illustrate this with simulations of a model studied in detail by Freire et al. [Macromolecules 19, 452 (1986) and later work]. copyright 1997 The American Physical Society

  11. Methods for nondestructive assay holdup measurements in shutdown uranium enrichment facilities

    International Nuclear Information System (INIS)

    Hagenauer, R.C.; Mayer, R.L. II.

    1991-09-01

    Measurement surveys of uranium holdup using nondestructive assay (NDA) techniques are being conducted for shutdown gaseous diffusion facilities at the Oak Ridge K-25 Site (formerly the Oak Ridge Gaseous Diffusion Plant). When in operation, these facilities processed UF 6 with enrichments ranging from 0.2 to 93 wt % 235 U. Following final shutdown of all process facilities, NDA surveys were initiated to provide process holdup data for the planning and implementation of decontamination and decommissioning activities. A three-step process is used to locate and quantify deposits: (1) high-resolution gamma-ray measurements are performed to generally define the relative abundances of radioisotopes present, (2) sizable deposits are identified using gamma-ray scanning methods, and (3) the deposits are quantified using neutron measurement methods. Following initial quantitative measurements, deposit sizes are calculated; high-resolution gamma-ray measurements are then performed on the items containing large deposits. The quantitative estimates for the large deposits are refined on the basis of these measurements. Facility management is using the results of the survey to support a variety of activities including isolation and removal of large deposits; performing health, safety, and environmental analyses; and improving facility nuclear material control and accountability records. 3 refs., 1 tab

  12. A combined enrichment/polymerase chain reaction based method for the routine screening of Streptococcus agalactiae in pregnant women

    Directory of Open Access Journals (Sweden)

    F.M. Munari

    2012-03-01

    Full Text Available Group B Streptococcus (GBS is the most common cause of life-threatening infection in neonates. Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS colonization. The objective of this study was to compare the performance of a combined enrichment/PCR based method targeting the atr gene in relation to culture using enrichment with selective broth medium (standard method to identify the presence of GBS in pregnant women. Rectovaginal GBS samples from women at ≥36 weeks of pregnancy were obtained with a swab and analyzed by the two methods. A total of 89 samples were evaluated. The prevalence of positive results for GBS detection was considerable higher when assessed by the combined enrichment/PCR method than with the standard method (35.9% versus 22.5%, respectively. The results demonstrated that the use of selective enrichment broth followed by PCR targeting the atr gene is a highly sensitive, specific and accurate test for GBS screening in pregnant women, allowing the detection of the bacteria even in lightly colonized patients. This PCR methodology may provide a useful diagnostic tool for GBS detection and contributes for a more accurate and effective intrapartum antibiotic and lower newborn mortality and morbidity.

  13. The separation nozzle method for enrichment of the light uranium isotope

    International Nuclear Information System (INIS)

    Becker, E.W.

    1982-05-01

    The history of the development of the separation nozzle method for enrichment of the light uranium isotope is described as a contribution to a memorandum published by Deutsche Forschungsgemeinschaft. The work was triggered off by an effect which had been observed in fundamental studies on gas kinetics. Development up to the technical maturity covered a period of more than 25 years. The implementation of the project at the Karlsruhe Nuclear Research Center provided an adequate financial and technical framework, the employment of senior staff without limitations in time being of major importance for the continuity of work. The links established between the Institute and the University and the resulting opportunity of having doctoral theses written, on the other hand, gave rise to a permanent flow of young, highly qualified scientists and engineers. Thus the Institut's requirements for junior staff could be satisfied in an optimum way. Although the Center offered a variety of possibilities of internal technical cooperation, important developments were performed jointly with industrial firms experienced in related fields. By this, not only a steady flow of know-how had been accumulated but also the large-scale applicability was ensured at a later date of results jointly obtained. (orig.) [de

  14. Rehydration properties of hybrid method dried fruit enriched by natural components

    Science.gov (United States)

    Kowalska, Hanna; Marzec, Agata; Kowalska, Jolanta; Ciurzyńska, Agnieszka; Samborska, Kinga; Bialik, Michał; Lenart, Andrzej

    2018-04-01

    The aim of the study was to determine the impact of osmotic pre-dehydration and drying of fruit on the rehydration properties of dried fruit. Herein, the effect of fruit juice, applied as a natural enriching substance was very important. In addition, the properties of dried fruits obtained through combined air-drying and subsequent microwave-vacuum drying with `puffing' effect were similar to the freeze-dried fruits, but showed other rehydration properties. As raw material, frozen strawberry (Honeoye variety) and fresh apples (Idared variety) were used in the study. The apples and partially defrosted strawberries were prior dehydrated in solutions of sucrose and a mixture of sucrose with chokeberry juice concentrate at 50°C for 2 h. Next, the fruit samples were dried by one of two ways: air-drying (50°C, 5 h) and microwavevacuum drying for about 360 s; and freeze-drying (30°C, 63 Pa, 24 h). The rehydration was carried out in distilled water (20°C, 5 h). The osmotic pre-dehydration hindered fruit drying process. The impact of drying method became particularly evident while examining the kinetics of rehydration. During the rehydration of the pre-dehydrated dried fruit a slower hydration could be observed. Freeze-dried strawberries absorbed 2-3 times more water than those dried by the `puffing' effect.

  15. Variational multiscale enrichment method with mixed boundary conditions for elasto-viscoplastic problems

    Science.gov (United States)

    Zhang, Shuhai; Oskay, Caglar

    2015-04-01

    This manuscript presents the formulation and implementation of the variational multiscale enrichment (VME) method for the analysis of elasto-viscoplastic problems. VME is a global-local approach that allows accurate fine scale representation at small subdomains, where important physical phenomena are likely to occur. The response within far-fields is idealized using a coarse scale representation. The fine scale representation not only approximates the coarse grid residual, but also accounts for the material heterogeneity. A one-parameter family of mixed boundary conditions that range from Dirichlet to Neumann is employed to study the effect of the choice of the boundary conditions at the fine scale on accuracy. The inelastic material behavior is modeled using Perzyna type viscoplasticity coupled with flow stress evolution idealized by the Johnson-Cook model. Numerical verifications are performed to assess the performance of the proposed approach against the direct finite element simulations. The results of verification studies demonstrate that VME with proper boundary conditions accurately model the inelastic response accounting for material heterogeneity.

  16. Benchmarking Methods and Data Sets for Ligand Enrichment Assessment in Virtual Screening

    Science.gov (United States)

    Xia, Jie; Tilahun, Ermias Lemma; Reid, Terry-Elinor; Zhang, Liangren; Wang, Xiang Simon

    2014-01-01

    Retrospective small-scale virtual screening (VS) based on benchmarking data sets has been widely used to estimate ligand enrichments of VS approaches in the prospective (i.e. real-world) efforts. However, the intrinsic differences of benchmarking sets to the real screening chemical libraries can cause biased assessment. Herein, we summarize the history of benchmarking methods as well as data sets and highlight three main types of biases found in benchmarking sets, i.e. “analogue bias”, “artificial enrichment” and “false negative”. In addition, we introduced our recent algorithm to build maximum-unbiased benchmarking sets applicable to both ligand-based and structure-based VS approaches, and its implementations to three important human histone deacetylase (HDAC) isoforms, i.e. HDAC1, HDAC6 and HDAC8. The Leave-One-Out Cross-Validation (LOO CV) demonstrates that the benchmarking sets built by our algorithm are maximum-unbiased in terms of property matching, ROC curves and AUCs. PMID:25481478

  17. Isotope enrichment

    International Nuclear Information System (INIS)

    Garbuny, M.

    1979-01-01

    The invention discloses a method for deriving, from a starting material including an element having a plurality of isotopes, derived material enriched in one isotope of the element. The starting material is deposited on a substrate at less than a critical submonatomic surface density, typically less than 10 16 atoms per square centimeter. The deposit is then selectively irradiated by a laser (maser or electronic oscillator) beam with monochromatic coherent radiation resonant with the one isotope causing the material including the one istope to escape from the substrate. The escaping enriched material is then collected. Where the element has two isotopes, one of which is to be collected, the deposit may be irradiated with radiation resonant with the other isotope and the residual material enriched in the one isotope may be evaporated from the substrate and collected

  18. Application and comparison of large-scale solution-based DNA capture-enrichment methods on ancient DNA

    DEFF Research Database (Denmark)

    Avila Arcos, Maria del Carmen; Cappellini, Enrico; Romero-Navarro, J. Alberto

    2011-01-01

    The development of second-generation sequencing technologies has greatly benefitted the field of ancient DNA (aDNA). Its application can be further exploited by the use of targeted capture-enrichment methods to overcome restrictions posed by low endogenous and contaminating DNA in ancient samples...

  19. Method of fault diagnosis in nozzle cascades for U-235 enrichment

    International Nuclear Information System (INIS)

    Schuette, R.; Steinhaus, H.

    1978-09-01

    In a separation nozzle cascade for enrichment of the light uranium isotope U-235 some 450 stages are connected in series. For optimum separation performance of such a plant the design values of the nozzle inlet pressure, of the UF 6 concentration of the UF 6 -cut and the cut of the light additional gas must be matched in all separation stages. Also the feed stream, the product stream, and the tails stream have to be controlled according to the cascade design values. Since it is not possible to measure the cuts directly, these values are calculated on the basis of the material flow balances of the cascade using the pressure values and the UF 6 concentration measurements in each stage, these data being supplemented by concentration measurements in the light and heavy fractions of selected stages. This approach requires the use of a digital computer for processing some 1500 readings to calculate the 2500 plant parameters defining the plant state. This study describes a method of diagnosing the major faults to be expected in a separation nozzle cascade. It is based on the fact that the fault profiles are characterized sufficiently well by maximum values and values to identify the cause of a fault and localize the point where it occurs by means of simple relations between these six values and of their relative positions. The performance of the method has been tested in experiments in the ten-stage pilot plant. For use in commercial separation nozzle cascades the range of performance and the special mode of implementation can be derived from the characteristics of the plant components (separation nozzles, compressors, control valves). The methodological approach in this fault diagnosis also provides the basis for computer aided control procedures to raise a separation cascade from any steady plant condition to its set point operation. (orig./HP) 891 HP [de

  20. Comparison of Six DNA Extraction Procedures and the Application of Plastid DNA Enrichment Methods in Selected Non-photosynthetic Plants

    Directory of Open Access Journals (Sweden)

    Shin-Yi Shyu

    2013-12-01

    Full Text Available Genomic DNA was isolated using three DNA extraction commercial kits and three CTAB-based methods for two non-photosynthetic plants, Balanophora japonica and Mitrastemon kanehirai. The quality of the isolated DNA was evaluated and subjected to following restriction enzyme digestions. All six procedures yielded DNA of sufficient quality for PCR, and the method described by Barnwell et al. (1998 performed well in isolating DNA from both species for restriction enzyme digestion. In addition, we succeeded to enrich plastid DNA content by using the methods depending on a high salt buffer to deplete nuclear material. The ‘high salt’ methods based on protocol presented by Milligan (1989 were able to increase plastid DNA effectively and significantly reduce nuclear DNA from M. kanehirai. The plastid DNA enrichment protocols are inexpensive and not time-consuming, and may be applicable to other non-photosynthetic plants.

  1. Active method of neutron time correlation coincidence measurement to authenticate mass and enrichment of uranium metal

    International Nuclear Information System (INIS)

    Zhang Songbai; Wu Jun; Zhu Jianyu; Tian Dongfeng; Xie Dong

    2011-01-01

    The active methodology of time correlation coincidence measurement of neutron is an effective verification means to authenticate uranium metal. A collimated 252 Cf neutron source was used to investigate mass and enrichment of uranium metal through the neutron transport simulation for different enrichments and different masses of uranium metal, then time correlation coincidence counts of them were obtained. By analyzing the characteristic of time correlation coincidence counts, the monotone relationships were founded between FWTH of time correlation coincidence and multiplication factor, between the total coincidence counts in FWTH for time correlation coincidence and mass of 235 U multiplied by multiplication factor, and between the ratio of neutron source penetration and mass of uranium metal. Thus the methodology to authenticate mass and enrichment of uranium metal was established with time correlation coincidence by active neutron investigation. (authors)

  2. Analysis of copper-nickel ores by gamma-gamma method in ore enrichment works

    International Nuclear Information System (INIS)

    Bol'shakov, A.Yu.; Tovstenko, Yu.G.; Chinskij, E.B.; Eliseev, G.I.

    1973-01-01

    The paper presents experimental data on continuous gamma-gamma assay of copper-nickel ores on conveyor belts and of dry discrete samples of classifier overflow at the enrichment plants of the Pechenganikel' group. The relative errors are given of the results of comparison of two-hour rapid analyses and shift and 24-hour chemical analyses of classifier overflow samples with the figures for gamma-gamma assay. The factors affecting the accuracy of the latter technique are elucidated. Practical recommendations are given on the use of this technique at the above enrichment plants. (author)

  3. Methods applied in the large littoral mesocosms study of nutrient enrichment in rocky shore ecosystems - EULIT

    NARCIS (Netherlands)

    Bokn, T.L.; Hoell, E.E.; Kersting, K.; Moy, F.E.; Sorensen, K.

    2001-01-01

    Eight concrete land-based mesocosms have been set up for a study of the effect of nutrient enrichment on littoral hard bottom ecosystems. The construction of the mesocosms and the community establishment were initiated 2 yr ahead of the MAST-III project. The littoral communities were established by

  4. Evaluating an Enrichment Program in Early Childhood: A Multi-Methods Approach

    Science.gov (United States)

    van Aswegen, Christa; Pendergast, Donna

    2015-01-01

    This article reports on the evaluation of one topic in an enrichment program designed for children in their early years of learning. The program is responsive to an increased understanding of the benefits for very young children of programs that not only take advantage of the sensitive periods for learning but that also assist parents to a take a…

  5. Enrichment Wastes' Processing of Manganiferous Ores with the Use of Mechanochemical Methods

    Science.gov (United States)

    Kubekova, Sholpan N.; Kapralova, Viktoria I.; Ibraimova, Gulnur T.; Batyrbayeva, Aigul A.

    2016-01-01

    The aim of the research is the study of the chemical and phase composition of enrichment wastes of manganiferous ore in Ushkatyn-III deposit and the synthesis of new materials by mechanochemical activation and subsequent heat treatment of the mechanical activation products. The use of XFA, infrared spectroscopy and electron probe microanalysis…

  6. Designing Focused Chemical Libraries Enriched in Protein-Protein Interaction Inhibitors using Machine-Learning Methods

    Science.gov (United States)

    Reynès, Christelle; Host, Hélène; Camproux, Anne-Claude; Laconde, Guillaume; Leroux, Florence; Mazars, Anne; Deprez, Benoit; Fahraeus, Robin; Villoutreix, Bruno O.; Sperandio, Olivier

    2010-01-01

    Protein-protein interactions (PPIs) may represent one of the next major classes of therapeutic targets. So far, only a minute fraction of the estimated 650,000 PPIs that comprise the human interactome are known with a tiny number of complexes being drugged. Such intricate biological systems cannot be cost-efficiently tackled using conventional high-throughput screening methods. Rather, time has come for designing new strategies that will maximize the chance for hit identification through a rationalization of the PPI inhibitor chemical space and the design of PPI-focused compound libraries (global or target-specific). Here, we train machine-learning-based models, mainly decision trees, using a dataset of known PPI inhibitors and of regular drugs in order to determine a global physico-chemical profile for putative PPI inhibitors. This statistical analysis unravels two important molecular descriptors for PPI inhibitors characterizing specific molecular shapes and the presence of a privileged number of aromatic bonds. The best model has been transposed into a computer program, PPI-HitProfiler, that can output from any drug-like compound collection a focused chemical library enriched in putative PPI inhibitors. Our PPI inhibitor profiler is challenged on the experimental screening results of 11 different PPIs among which the p53/MDM2 interaction screened within our own CDithem platform, that in addition to the validation of our concept led to the identification of 4 novel p53/MDM2 inhibitors. Collectively, our tool shows a robust behavior on the 11 experimental datasets by correctly profiling 70% of the experimentally identified hits while removing 52% of the inactive compounds from the initial compound collections. We strongly believe that this new tool can be used as a global PPI inhibitor profiler prior to screening assays to reduce the size of the compound collections to be experimentally screened while keeping most of the true PPI inhibitors. PPI-HitProfiler is

  7. Designing focused chemical libraries enriched in protein-protein interaction inhibitors using machine-learning methods.

    Directory of Open Access Journals (Sweden)

    Christelle Reynès

    2010-03-01

    Full Text Available Protein-protein interactions (PPIs may represent one of the next major classes of therapeutic targets. So far, only a minute fraction of the estimated 650,000 PPIs that comprise the human interactome are known with a tiny number of complexes being drugged. Such intricate biological systems cannot be cost-efficiently tackled using conventional high-throughput screening methods. Rather, time has come for designing new strategies that will maximize the chance for hit identification through a rationalization of the PPI inhibitor chemical space and the design of PPI-focused compound libraries (global or target-specific. Here, we train machine-learning-based models, mainly decision trees, using a dataset of known PPI inhibitors and of regular drugs in order to determine a global physico-chemical profile for putative PPI inhibitors. This statistical analysis unravels two important molecular descriptors for PPI inhibitors characterizing specific molecular shapes and the presence of a privileged number of aromatic bonds. The best model has been transposed into a computer program, PPI-HitProfiler, that can output from any drug-like compound collection a focused chemical library enriched in putative PPI inhibitors. Our PPI inhibitor profiler is challenged on the experimental screening results of 11 different PPIs among which the p53/MDM2 interaction screened within our own CDithem platform, that in addition to the validation of our concept led to the identification of 4 novel p53/MDM2 inhibitors. Collectively, our tool shows a robust behavior on the 11 experimental datasets by correctly profiling 70% of the experimentally identified hits while removing 52% of the inactive compounds from the initial compound collections. We strongly believe that this new tool can be used as a global PPI inhibitor profiler prior to screening assays to reduce the size of the compound collections to be experimentally screened while keeping most of the true PPI inhibitors. PPI

  8. Designing focused chemical libraries enriched in protein-protein interaction inhibitors using machine-learning methods.

    Science.gov (United States)

    Reynès, Christelle; Host, Hélène; Camproux, Anne-Claude; Laconde, Guillaume; Leroux, Florence; Mazars, Anne; Deprez, Benoit; Fahraeus, Robin; Villoutreix, Bruno O; Sperandio, Olivier

    2010-03-05

    Protein-protein interactions (PPIs) may represent one of the next major classes of therapeutic targets. So far, only a minute fraction of the estimated 650,000 PPIs that comprise the human interactome are known with a tiny number of complexes being drugged. Such intricate biological systems cannot be cost-efficiently tackled using conventional high-throughput screening methods. Rather, time has come for designing new strategies that will maximize the chance for hit identification through a rationalization of the PPI inhibitor chemical space and the design of PPI-focused compound libraries (global or target-specific). Here, we train machine-learning-based models, mainly decision trees, using a dataset of known PPI inhibitors and of regular drugs in order to determine a global physico-chemical profile for putative PPI inhibitors. This statistical analysis unravels two important molecular descriptors for PPI inhibitors characterizing specific molecular shapes and the presence of a privileged number of aromatic bonds. The best model has been transposed into a computer program, PPI-HitProfiler, that can output from any drug-like compound collection a focused chemical library enriched in putative PPI inhibitors. Our PPI inhibitor profiler is challenged on the experimental screening results of 11 different PPIs among which the p53/MDM2 interaction screened within our own CDithem platform, that in addition to the validation of our concept led to the identification of 4 novel p53/MDM2 inhibitors. Collectively, our tool shows a robust behavior on the 11 experimental datasets by correctly profiling 70% of the experimentally identified hits while removing 52% of the inactive compounds from the initial compound collections. We strongly believe that this new tool can be used as a global PPI inhibitor profiler prior to screening assays to reduce the size of the compound collections to be experimentally screened while keeping most of the true PPI inhibitors. PPI-HitProfiler is

  9. Large-scale systematic analysis of 2D fingerprint methods and parameters to improve virtual screening enrichments.

    Science.gov (United States)

    Sastry, Madhavi; Lowrie, Jeffrey F; Dixon, Steven L; Sherman, Woody

    2010-05-24

    A systematic virtual screening study on 11 pharmaceutically relevant targets has been conducted to investigate the interrelation between 8 two-dimensional (2D) fingerprinting methods, 13 atom-typing schemes, 13 bit scaling rules, and 12 similarity metrics using the new cheminformatics package Canvas. In total, 157 872 virtual screens were performed to assess the ability of each combination of parameters to identify actives in a database screen. In general, fingerprint methods, such as MOLPRINT2D, Radial, and Dendritic that encode information about local environment beyond simple linear paths outperformed other fingerprint methods. Atom-typing schemes with more specific information, such as Daylight, Mol2, and Carhart were generally superior to more generic atom-typing schemes. Enrichment factors across all targets were improved considerably with the best settings, although no single set of parameters performed optimally on all targets. The size of the addressable bit space for the fingerprints was also explored, and it was found to have a substantial impact on enrichments. Small bit spaces, such as 1024, resulted in many collisions and in a significant degradation in enrichments compared to larger bit spaces that avoid collisions.

  10. Uranium enrichment. Enrichment processes

    International Nuclear Information System (INIS)

    Alexandre, M.; Quaegebeur, J.P.

    2009-01-01

    Despite the remarkable progresses made in the diversity and the efficiency of the different uranium enrichment processes, only two industrial processes remain today which satisfy all of enriched uranium needs: the gaseous diffusion and the centrifugation. This article describes both processes and some others still at the demonstration or at the laboratory stage of development: 1 - general considerations; 2 - gaseous diffusion: physical principles, implementation, utilisation in the world; 3 - centrifugation: principles, elementary separation factor, flows inside a centrifuge, modeling of separation efficiencies, mechanical design, types of industrial centrifuges, realisation of cascades, main characteristics of the centrifugation process; 4 - aerodynamic processes: vortex process, nozzle process; 5 - chemical exchange separation processes: Japanese ASAHI process, French CHEMEX process; 6 - laser-based processes: SILVA process, SILMO process; 7 - electromagnetic and ionic processes: mass spectrometer and calutron, ion cyclotron resonance, rotating plasmas; 8 - thermal diffusion; 9 - conclusion. (J.S.)

  11. Method for estimate the economic characteristics of an uranium enrichment plant by gaseous diffusion

    International Nuclear Information System (INIS)

    Berault, J.C.

    1975-01-01

    To estimate the economic characteristics of an uranium enrichment plant by gaseous diffusion is to determine the prospective price of the separative work unit to which leads the concerned technology, and to collect the data allowing to ascertain that this price remains in the area of development of the prices forecasted by the other projects. The prospective price estimated by the promoter is the synthesis of the components of the go decision and which are a potential market and a comprehensive industrially proven plant design, including the basic economic and technical data of the project. Procedures for estimating these components and their synthesis, exclusive of financing problems are reviewed [fr

  12. Sistema ultra-sonico para medida volumetrica

    OpenAIRE

    Isaias Ferreira da Silva

    1999-01-01

    Resumo: Um método inovador para a medida do volume de um líquido dentro de um recipiente fechado é proposto. A motivação deste trabalho é a aplicação do método para a medida do volume de combustível dentro de um tanque de automóvel. Este método se baseia no fenômeno de reverberação sonora que relaciona o tempo de decaimento da intensidade do som dentro de um ambiente com o seu volume. Este tempo, conhecido como tempo de reverberação, está associado ao volume vazio dentro do recipiente, ao mat...

  13. Medidas de la felicidad nacional bruta

    NARCIS (Netherlands)

    R. Veenhoven (Ruut)

    2009-01-01

    textabstractResumen. La felicidad está abriéndose hueco en la agenda política, lo cual reclama medir el éxito alcanzado por los distintos países en crear felicidad para un gran número de personas, de un modo análogo a las medidas del éxito en la creación de riqueza, como el PNB. La felicidad se

  14. Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues

    Directory of Open Access Journals (Sweden)

    Blachutzik Jörg O

    2012-08-01

    Full Text Available Abstract Background Sterols and Sphingolipids form lipid clusters in the plasma membranes of cell types throughout the animal and plant kingdoms. These lipid domains provide a medium for protein signaling complexes at the plasma membrane and are also observed to be principal regions of membrane contact at the inception of infection. We visualized different specific fluorescent lipophilic stains of the both sphingolipid enriched and non-sphingolipid enriched regions in the plasma membranes of live protoplasts of Arabidopsis thaliana. Results Lipid staining protocols for several fluorescent lipid analogues in plants are presented. The most emphasis was placed on successful protocols for the single and dual staining of sphingolipid enriched regions and exclusion of sphingolipid enriched regions on the plasma membrane of Arabidopsis thaliana protoplasts. A secondary focus was placed to ensure that these staining protocols presented still maintain cell viability. Furthermore, the protocols were successfully tested with the spectrally sensitive dye Laurdan. Conclusion Almost all existing staining procedures of the plasma membrane with fluorescent lipid analogues are specified for animal cells and tissues. In order to develop lipid staining protocols for plants, procedures were established with critical steps for the plasma membrane staining of Arabidopsis leaf tissue and protoplasts. The success of the plasma membrane staining protocols was additionally verified by measurements of lipid dynamics by the fluorescence recovery after photobleaching technique and by the observation of new phenomena such as time dependent lipid polarization events in living protoplasts, for which a putative physiological relevance is suggested.

  15. Alkaline Peptone Water-Based Enrichment Method for mcr-3 From Acute Diarrheic Outpatient Gut Samples

    Directory of Open Access Journals (Sweden)

    Qiaoling Sun

    2018-05-01

    Full Text Available A third plasmid-mediated colistin resistance gene, mcr-3, is increasingly being reported in Enterobacteriaceae and Aeromonas spp. from animals and humans. To investigate the molecular epidemiology of mcr in the gut flora of Chinese outpatients, 152 stool specimens were randomly collected from outpatients in our hospital from May to June, 2017. Stool specimens enriched in alkaline peptone water or Luria-Bertani (LB broth were screened for mcr-1, mcr-2, and mcr-3 using polymerase chain reaction (PCR-based assays. Overall, 19.1% (29/152 and 5.3% (8/152 of the stool samples enriched in alkaline peptone water were PCR-positive for mcr-1 and mcr-3, respectively, while 2.7% (4/152 of samples were positive for both mcr-1 and mcr-3. Strains isolated from the samples that were both mcr-1- and mcr-3-positive were subjected to antimicrobial susceptibility testing by broth microdilution. They were also screened for the presence of other resistance genes by PCR, while multilocus sequence typing and whole-genome sequencing were used to investigate the molecular epidemiology and genetic environment, respectively, of the resistance genes. mcr-3-positive Aeromonas veronii strain 126-14, containing a mcr-3.8-mcr-3-like2 segment, and mcr-1-positive Escherichia coli strain 126-1, belonging to sequence type 1485, were isolated from the sample from a diarrheic butcher with no history of colistin treatment. A. veronii 126-14 had a colistin minimum inhibitory concentration (MIC of 2 µg/mL and was susceptible to antibiotics in common use, while E. coli 126-1 produced TEM-1, CTX-M-55, and CTX-M-14 β-lactamases and was resistant to colistin, ceftazidime, and cefotaxime. Overall, there was a higher detection rate of mcr-3-carrying strains with low colistin MICs from the samples enriched in alkaline peptone water than from samples grown in LB broth.

  16. Method for the preparation of deuterium-enriched water in the preparation of hydrogen

    International Nuclear Information System (INIS)

    Mandrin, C.

    1984-01-01

    The synthesis-gas plant is operated in a number of successive operating periods such that during the first operating period an excess of fresh water is supplied to the synthesis gas plant and the waste water from the resulting mixture is separated in a condenser and supplied to a storage container. During the subsequent operating periods, the waste water stored in the container is used as the feed water for the synthesis gas plant and the waste water obtained on each occasion is stored in the container with increasing deuterium concentration. The waste water obtained during the last operating period and having the highest deuterium concentration is used to feed a plant for producing heavy water. This process, when used in a synthesis gas plant for producing hydrogen, can be used to obtain deuterium-enriched water as a feedstock for a heavy-water plant without expensive additional energy-consuming devices

  17. Solvent extraction as a method of promoting uranium enrichment by chemical exchange

    International Nuclear Information System (INIS)

    Fathurrachman.

    1995-01-01

    This thesis examines a chemical exchange process for uranium enrichment using solvent extraction. The system selected is the isotope exchange for uranium species in the form of uranous and uranyl chloride complexes. Solvent extraction has been studied before by French workers for this application but little was published on this. Much of this present work is therefore novel. The equilibrium data for the extraction of U(IV) as U 4+ and U(VI) as UO 2 2+ from hydrochloric media into an organic phase containing tri-n-octylamine (TOA) in benzene is given. Benzene is used to prevent third phase formation. In 4 M HCl U(VI) was found to be very soluble in the organic phase but U(IV) was virtually insoluble. Most of the equilibrium data has been correlated by the Langmuir isotherm. This thesis also outlines the methodology that has to be used to design a plant based on this process. (author)

  18. Neutron source, linear-accelerator fuel enricher and regenerator and associated methods

    Science.gov (United States)

    Steinberg, Meyer; Powell, James R.; Takahashi, Hiroshi; Grand, Pierre; Kouts, Herbert

    1982-01-01

    A device for producing fissile material inside of fabricated nuclear elements so that they can be used to produce power in nuclear power reactors. Fuel elements, for example, of a LWR are placed in pressure tubes in a vessel surrounding a liquid lead-bismuth flowing columnar target. A linear-accelerator proton beam enters the side of the vessel and impinges on the dispersed liquid lead-bismuth columns and produces neutrons which radiate through the surrounding pressure tube assembly or blanket containing the nuclear fuel elements. These neutrons are absorbed by the natural fertile uranium-238 elements and are transformed to fissile plutonium-239. The fertile fuel is thus enriched in fissile material to a concentration whereby they can be used in power reactors. After use in the power reactors, dispensed depleted fuel elements can be reinserted into the pressure tubes surrounding the target and the nuclear fuel regenerated for further burning in the power reactor.

  19. Effects of four different cooking methods on some quality characteristics of low fat Inegol meatball enriched with flaxseed flour.

    Science.gov (United States)

    Turp, Gulen Yildiz

    2016-11-01

    The present study is concerned with the effects of four different cooking methods (grill, oven, pan and ohmic cooking) on physicochemical parameters (cooking yield moisture retention, fat retention, color, texture), fatty acid composition and sensory characteristics of low fat Turkish traditional Inegol meatball. Flaxseed flour was used as a fat substitute in the production of meatballs. Meatball proximate composition was affected by the cooking methods mainly as a consequence of the weight losses. The highest cooking yield was found in samples cooked in the oven. Flaxseed flour contains high amount of α-linolenic acid and ohmic cooking seems to be the best cooking method in terms of retaining this fatty acid in meatballs enriched with flaxseed flour. However ohmic cooked meatball samples had a brighter surface color and harder texture in comparison with meatball samples cooked via traditional methods. There was no significant difference between the sensory evaluation scores of meatballs. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Profiling plasma extracellular vesicle by pluronic block-copolymer based enrichment method unveils features associated with breast cancer aggression, metastasis and invasion.

    Science.gov (United States)

    Zhong, Zhenyu; Rosenow, Matthew; Xiao, Nick; Spetzler, David

    2018-01-01

    Extracellular vesicle (EV)-based liquid biopsies have been proposed to be a readily obtainable biological substrate recently for both profiling and diagnostics purposes. Development of a fast and reliable preparation protocol to enrich such small particles could accelerate the discovery of informative, disease-related biomarkers. Though multiple EV enrichment protocols are available, in terms of efficiency, reproducibility and simplicity, precipitation-based methods are most amenable to studies with large numbers of subjects. However, the selectivity of the precipitation becomes critical. Here, we present a simple plasma EV enrichment protocol based on pluronic block copolymer. The enriched plasma EV was able to be verified by multiple platforms. Our results showed that the particles enriched from plasma by the copolymer were EV size vesicles with membrane structure; proteomic profiling showed that EV-related proteins were significantly enriched, while high-abundant plasma proteins were significantly reduced in comparison to other precipitation-based enrichment methods. Next-generation sequencing confirmed the existence of various RNA species that have been observed in EVs from previous studies. Small RNA sequencing showed enriched species compared to the corresponding plasma. Moreover, plasma EVs enriched from 20 advanced breast cancer patients and 20 age-matched non-cancer controls were profiled by semi-quantitative mass spectrometry. Protein features were further screened by EV proteomic profiles generated from four breast cancer cell lines, and then selected in cross-validation models. A total of 60 protein features that highly contributed in model prediction were identified. Interestingly, a large portion of these features were associated with breast cancer aggression, metastasis as well as invasion, consistent with the advanced clinical stage of the patients. In summary, we have developed a plasma EV enrichment method with improved precipitation selectivity

  1. Analysis methods and performance of an automated system for measuring both concentration and enrichment of uranium in solutions

    International Nuclear Information System (INIS)

    Kelley, T.A.; Parker, J.L.; Sampson, T.E.

    1993-01-01

    For the 1992 INNM meeting, the authors reported on the general characteristics of an automated system--then under development--for measuring both the concentration and enrichment of uranium in solutions. That paper emphasized the automated control capability, the measurement sequences, and safety features of the system. In this paper, the authors report in detail on the measurement methods, the analysis algorithms, and the performance of the delivered system. The uranium concentration is measured by a transmission-corrected X-ray fluorescence method. Cobalt-57 is the fluorescing source and a combined 153 Gd and 57 Co source is used for the transmission measurements. Corrections are made for both the absorption of the exciting 57 Co gamma rays and the excited uranium X-rays. The 235 U concentration is measured by a transmission-corrected method, which employs the 185.7-keV gamma ray of 235 U and a transmission source of 75 Se to make corrections for the self-absorption of the 235 U gamma rays in the solution samples. Both measurements employ high-resolution gamma-ray spectrometry and use the same 50ml sample contained in a custom-molded, flat-bottomed, polypropylene bottle. Both measurements are intended for uranium solutions with concentrations ≥0.1 g U/l, although at higher enrichments the passive measurement will be even more sensitive

  2. A Facile Method for Separating and Enriching Nano and Submicron Particles from Titanium Dioxide Found in Food and Pharmaceutical Products

    Science.gov (United States)

    Yang, Yu; Capco, David G.; Westerhoff, Paul

    2016-01-01

    Recent studies indicate the presence of nano-scale titanium dioxide (TiO2) as an additive in human foodstuffs, but a practical protocol to isolate and separate nano-fractions from soluble foodstuffs as a source of material remains elusive. As such, we developed a method for separating the nano and submicron fractions found in commercial-grade TiO2 (E171) and E171 extracted from soluble foodstuffs and pharmaceutical products (e.g., chewing gum, pain reliever, and allergy medicine). Primary particle analysis of commercial-grade E171 indicated that 54% of particles were nano-sized (i.e., < 100 nm). Isolation and primary particle analysis of five consumer goods intended to be ingested revealed differences in the percent of nano-sized particles from 32%‒58%. Separation and enrichment of nano- and submicron-sized particles from commercial-grade E171 and E171 isolated from foodstuffs and pharmaceuticals was accomplished using rate-zonal centrifugation. Commercial-grade E171 was separated into nano- and submicron-enriched fractions consisting of a nano:submicron fraction of approximately 0.45:1 and 3.2:1, respectively. E171 extracted from gum had nano:submicron fractions of 1.4:1 and 0.19:1 for nano- and submicron-enriched, respectively. We show a difference in particle adhesion to the cell surface, which was found to be dependent on particle size and epithelial orientation. Finally, we provide evidence that E171 particles are not immediately cytotoxic to the Caco-2 human intestinal epithelium model. These data suggest that this separation method is appropriate for studies interested in isolating the nano-sized particle fraction taken directly from consumer products, in order to study separately the effects of nano and submicron particles. PMID:27798677

  3. A study of industrial exposure to uranium aerosols from the laser enrichment procedure - methods and results

    International Nuclear Information System (INIS)

    Ansoborlo, E.; Claraz, M.; Henge-Napoli, M.H.; Metivier, H.

    1995-01-01

    Comprehensive studies of the radiotoxicological risk at new uranium enrichment processing facilities using laser isotopic separation, were particularly motivated by the generation of a uranium oxide aerosol identified as UO 2 + U metal . Taking the new ICRP 66 recommendations into account, the following study on this uranium oxide mixture, was aimed at determining the physico-chemical and biokinetic specific parameters required in order to calculate the effective dose. The activity median aerodynamic diameters (AMAD) ranged between 5.2 and 10 μm with, in some cases, up to 20% of submicron size particles, while concentration values at the workplace ranged from 1.8 to 125 Bq m -3 and biological half-time calculations gave a 48 d period with in vitro dissolution test and a 77 d period with in vivo inhalation experiments. Transfer rates and dissolution rates obtained from both in vitro and vivo experiments intend to emphasize a class W behaviour in term of ICRP 30 and M in term of ICRP 66. (authors). 3 figs., 4 tabs., 22 refs

  4. A method and software framework for enriching private biomedical sources with data from public online repositories.

    Science.gov (United States)

    Anguita, Alberto; García-Remesal, Miguel; Graf, Norbert; Maojo, Victor

    2016-04-01

    Modern biomedical research relies on the semantic integration of heterogeneous data sources to find data correlations. Researchers access multiple datasets of disparate origin, and identify elements-e.g. genes, compounds, pathways-that lead to interesting correlations. Normally, they must refer to additional public databases in order to enrich the information about the identified entities-e.g. scientific literature, published clinical trial results, etc. While semantic integration techniques have traditionally focused on providing homogeneous access to private datasets-thus helping automate the first part of the research, and there exist different solutions for browsing public data, there is still a need for tools that facilitate merging public repositories with private datasets. This paper presents a framework that automatically locates public data of interest to the researcher and semantically integrates it with existing private datasets. The framework has been designed as an extension of traditional data integration systems, and has been validated with an existing data integration platform from a European research project by integrating a private biological dataset with data from the National Center for Biotechnology Information (NCBI). Copyright © 2016 Elsevier Inc. All rights reserved.

  5. Comprehensive profiling of retroviral integration sites using target enrichment methods from historical koala samples without an assembled reference genome

    Directory of Open Access Journals (Sweden)

    Pin Cui

    2016-03-01

    Full Text Available Background. Retroviral integration into the host germline results in permanent viral colonization of vertebrate genomes. The koala retrovirus (KoRV is currently invading the germline of the koala (Phascolarctos cinereus and provides a unique opportunity for studying retroviral endogenization. Previous analysis of KoRV integration patterns in modern koalas demonstrate that they share integration sites primarily if they are related, indicating that the process is currently driven by vertical transmission rather than infection. However, due to methodological challenges, KoRV integrations have not been comprehensively characterized. Results. To overcome these challenges, we applied and compared three target enrichment techniques coupled with next generation sequencing (NGS and a newly customized sequence-clustering based computational pipeline to determine the integration sites for 10 museum Queensland and New South Wales (NSW koala samples collected between the 1870s and late 1980s. A secondary aim of this study sought to identify common integration sites across modern and historical specimens by comparing our dataset to previously published studies. Several million sequences were processed, and the KoRV integration sites in each koala were characterized. Conclusions. Although the three enrichment methods each exhibited bias in integration site retrieval, a combination of two methods, Primer Extension Capture and hybridization capture is recommended for future studies on historical samples. Moreover, identification of integration sites shows that the proportion of integration sites shared between any two koalas is quite small.

  6. Application of biclustering of gene expression data and gene set enrichment analysis methods to identify potentially disease causing nanomaterials

    Directory of Open Access Journals (Sweden)

    Andrew Williams

    2015-12-01

    Full Text Available Background: The presence of diverse types of nanomaterials (NMs in commerce is growing at an exponential pace. As a result, human exposure to these materials in the environment is inevitable, necessitating the need for rapid and reliable toxicity testing methods to accurately assess the potential hazards associated with NMs. In this study, we applied biclustering and gene set enrichment analysis methods to derive essential features of altered lung transcriptome following exposure to NMs that are associated with lung-specific diseases. Several datasets from public microarray repositories describing pulmonary diseases in mouse models following exposure to a variety of substances were examined and functionally related biclusters of genes showing similar expression profiles were identified. The identified biclusters were then used to conduct a gene set enrichment analysis on pulmonary gene expression profiles derived from mice exposed to nano-titanium dioxide (nano-TiO2, carbon black (CB or carbon nanotubes (CNTs to determine the disease significance of these data-driven gene sets.Results: Biclusters representing inflammation (chemokine activity, DNA binding, cell cycle, apoptosis, reactive oxygen species (ROS and fibrosis processes were identified. All of the NM studies were significant with respect to the bicluster related to chemokine activity (DAVID; FDR p-value = 0.032. The bicluster related to pulmonary fibrosis was enriched in studies where toxicity induced by CNT and CB studies was investigated, suggesting the potential for these materials to induce lung fibrosis. The pro-fibrogenic potential of CNTs is well established. Although CB has not been shown to induce fibrosis, it induces stronger inflammatory, oxidative stress and DNA damage responses than nano-TiO2 particles.Conclusion: The results of the analysis correctly identified all NMs to be inflammogenic and only CB and CNTs as potentially fibrogenic. In addition to identifying several

  7. Medidas de pobreza: un enfoque alternativo

    OpenAIRE

    Lazzari, Luisa L; Fernández, María José

    2006-01-01

    Una medida creíble de pobreza e indigencia puede ser un poderoso instrumento para concentrar la atención del policymaker en las condiciones de vida de los pobres. Las opciones elegidas para fijarlas tienen gran importancia para las decisiones políticas a tomar. El método indirecto o enfoque del ingreso parte de la valorización de una Canasta Básica Alimentaria (CBA) que determina la cantidad de calorías mínimas necesarias para satisfacer las necesidades biológicas básicas de...

  8. Medidas de administración pesquera.

    OpenAIRE

    2014-01-01

    En el Titulo II de la Ley General de Pesca y Acuicultura "De la Administración de las Pesquerías", específicamente en facultades de conservación de los recursos hidrobiológicos, se establece lo siguiente: En cada área de pesca, independiente del régimen de acceso a que se encuentre sometida, el Ministerio, mediante decreto supremo fundado, podrá establecer una o más prohibiciones o medidas de administración de recursos hidrobiológicos. Esta presentación que proporciona información refere...

  9. Carcass enrichment detects Salmonella from broiler carcasses found to be negative by other sampling methods

    Science.gov (United States)

    The most frequently used methods to recover Salmonella from processed broiler chicken carcasses involve carcass rinsing or neck skin maceration. These methods are nondestructive and practical, but have limited sensitivity. The standard carcass rinse method uses only 7.5% of the residual rinsate an...

  10. Improved accountability method for measuring enriched uranium in H-Canyon dissolver solution at the Savannah River Site

    International Nuclear Information System (INIS)

    Maxwell, S.L. III; Satkowski, J.; Mahannah, R.N.

    1992-01-01

    At the Savannah River Site (SRS), accountability measurement of enriched uranium dissolved in H-Canyon is performed using isotope dilution mass spectrometry (IDMS). In the IDMS analytical method, a known quantity of uranium 233 is added to the sample solution containing enriched uranium and fission products. The resulting uranium mixture must first be purified using a separation technique in the shielded analytical(''hot'') cells to lower radioactivity levels by removing fission products. Following this purification, the sample is analyzed by mass spectrometry to determine the total uranium content and isotopic abundance. The magnitude of the response of each uranium isotope in the sample solution and the response of the U 233 spike is measured. By ratioing these responses, relative to the known quantity of the U 233 spike, the uranium content can be determined. A hexane solvent extraction technique, used for years at SRS to remove fission products prior to the mass spectrometry analysis of uranium, has several problems. The hexone method is tedious, requires additional sample clean-up after the purified sample is removed from the shielded cells and requires the use of Resource Conservation and Recovery Act (RCRA)-listed hazardous materials (hexone and chromium compounds). A new high speed separation method that enables a rapid removal of fission products in a shielded cells environment has been developed by the SRS Central Laboratory to replace the hexone method. The new high speed column extraction chromatography technique employs applied vacuum and columns containing tri (2-ethyl-hexyl) phosphate (TEHP) solvent coated on a small particle inert support (SM-7 Bio Beads). The new separation is rapid, user friendly, eliminates the use of the RCA-listed hazardous chemicals and reduces the amount of solid waste generated by the separation method. 2 tabs. 4 figs

  11. Rapid method for the sequential measurement of isotopes of Am and Pu in liquid matrices by alpha spectrometry; Método rápido para la medida secuencial de isótopos de Am y Pu en matrices líquidas por espectrometría alfa

    Energy Technology Data Exchange (ETDEWEB)

    Mantero Cabrera, J.

    2014-07-01

    : In radiological emergencies it's necessary a fast response from the laboratories in the quantification of certain radionuclides, in order to take decisions. In these cases, is the reaction time the key parameter to consider (without neglecting the quality of the measurement). In this work, it is shown a method for aqueous matrices that generates Pu and Am isotopes sources in one single day of work, to be measured subsequently by alpha spectrometry. The developed methodology has been validated through its application to reference samples and also taking part intercom- parison exercises, having in both cases, satisfactory results. This way, we check the validity of this fast method that let us generate in 24 hours (since the sample arrives our laboratory), one measurement with a Minimun Detectable Activity (MDA) of about 0.004Bq/L for Pu and Am isotopes in liquid matrices. [Spanish] En situaciones de emergencia radiológica es muy común la necesidad de dar una respuesta rápida por parte de los laboratorios en la cuantificación de ciertos radionucleidos, de cara a la toma de decisiones. En estos casos, el tiempo de reacción es una variable fundamental a considerar (aunque obviamente sin dejar de lado la calidad en el resultado de la medida). En este trabajo se presenta un método para matrices acuosas que permite generar en una única jornada de trabajo, las fuentes de medida necesarias en la detección de isótopos de Pu y Am para ser medidas con posterioridad por espectrometría alfa. La metodología puesta a punto ha sido validada mediante su aplicación a muestras de referencia y mediante la participación en ejercicios de intercomparación, en ambos casos, con resultados satisfactorios. Queda así demostrada la validez de este método rápido que nos permite generar en 24 horas desde la recepción de la muestra, una medida en nuestros sistemas con una Actividad Mínima Detectable (AMD) de 0.004 Bq/L tanto para isótopos de Pu (238Pu, 239+240Pu) como de

  12. Medidas tipográficas: un recuento

    Directory of Open Access Journals (Sweden)

    Oriol Moret Viñals

    2015-07-01

    Full Text Available Breve repaso al desarrollo de la concepción métrica en la tipografía en plomo, alternativa y complemento de la visión habitual en lo que conocemos como “tipometría”. Se parte de una noción amplia de medida: el factor cuantitativo se dilata con la articulación de relaciones proporcionales e integra la dimensión cualitativa de los sistemas métricos tradicionales. La tipografía se considera bajo presupuestos similares: como sistema articulado de piezas materiales cuyas magnitudes contienen usos y valores específicos, más allá de su simple expresión numérica. Con tales premisas, se revisan fuentes documentales significativas del “período de la imprenta manual”. Su examen, vinculado a los usos y costumbres de oficio, desvela la distinta aproximación métrica, que puede resumirse en tres fases evolutivas: hermética, intensiva, expansiva –como recogen las referencias de los cuerpos tipográficos: adjetivas, sustantivas, numéricas. Se proporciona así un marco para apreciar el cambio de sentido en las medidas tipográficas hasta nuestros días.

  13. Method of controlling the mercury vapor pressure in a photo-chemical lamp or vapor filter used for Hg.sup.196 enrichment

    Science.gov (United States)

    Grossman, Mark W.

    1993-01-01

    The present invention is directed to a method of eliminating the cold spot zones presently used on Hg.sup.196 isotope separation lamps and filters by the use of a mercury amalgams, preferably mercury - indium amalgams. The use of an amalgam affords optimization of the mercury density in the lamp and filter of a mercury enrichment reactor, particularly multilamp enrichment reactors. Moreover, the use of an amalgam in such lamps and/or filters affords the ability to control the spectral line width of radiation emitted from lamps, a requirement for mercury enrichment.

  14. Method of controlling the mercury vapor pressure in a photo-chemical lamp or vapor filter used for Hg[sup 196] enrichment

    Science.gov (United States)

    Grossman, M.W.

    1993-02-16

    The present invention is directed to a method of eliminating the cold spot zones presently used on Hg[sup 196] isotope separation lamps and filters by the use of a mercury amalgams, preferably mercury - indium amalgams. The use of an amalgam affords optimization of the mercury density in the lamp and filter of a mercury enrichment reactor, particularly multilamp enrichment reactors. Moreover, the use of an amalgam in such lamps and/or filters affords the ability to control the spectral line width of radiation emitted from lamps, a requirement for mercury enrichment.

  15. Method of controlling the mercury vapor pressure in a photo-chemical lamp or vapor filter used for Hg196 enrichment

    International Nuclear Information System (INIS)

    Grossman, M.W.

    1993-01-01

    The present invention is directed to a method of eliminating the cold spot zones presently used on Hg 196 isotope separation lamps and filters by the use of a mercury amalgams, preferably mercury - indium amalgams. The use of an amalgam affords optimization of the mercury density in the lamp and filter of a mercury enrichment reactor, particularly multilamp enrichment reactors. Moreover, the use of an amalgam in such lamps and/or filters affords the ability to control the spectral line width of radiation emitted from lamps, a requirement for mercury enrichment

  16. Enrichment of W2B5 from WO3 and B2O3 by Double SHS Method

    Directory of Open Access Journals (Sweden)

    Bora DERIN

    2018-02-01

    Full Text Available A second self-propagating high-temperature synthesis (SHS was carried out to enrich the W2B5 content in the SHS product containing a mixture of various tungsten boride compounds. In the experiment, the process called Double-SHS (D-SHS was conducted in two steps. In the first SHS reaction, an initial molar composition ratio of WO3:B2O3:Mg mixture was selected as 1:3:8. The product was then hot-leached with hydrochloric acid to eliminate MgO and Mg3B2O6 phases. The leached product, consisting of 72.6 wt.% W2B5, 16.1 wt.% WB, 8.4 wt.% W2B, and 2.9 wt.% W, was again reacted with the Mg and B2O3 mixture by second SHS. After another acid leaching step, W2B5 content in the D-SHS product was found to be 98.2 wt.%. The study showed that D-SHS is an effective method for boron enrichment in the tungsten compounds.DOI: http://dx.doi.org/10.5755/j01.ms.24.1.17834

  17. Qualitative Methods Can Enrich Quantitative Research on Occupational Stress: An Example from One Occupational Group

    Science.gov (United States)

    Schonfeld, Irvin Sam; Farrell, Edwin

    2010-01-01

    The chapter examines the ways in which qualitative and quantitative methods support each other in research on occupational stress. Qualitative methods include eliciting from workers unconstrained descriptions of work experiences, careful first-hand observations of the workplace, and participant-observers describing "from the inside" a…

  18. Uranium enrichment techniques

    International Nuclear Information System (INIS)

    Hamdoun, N.A.

    2007-01-01

    This article includes an introduction about the isotopes of natural uranium, their existence and the difficulty of the separation between them. Then it goes to the details of a number of methods used to enrich uranium: Gaseous Diffusion method, Electromagnetic method, Jet method, Centrifugal method, Chemical method, Laser method and Plasma method.

  19. Advanced enrichment techniques

    International Nuclear Information System (INIS)

    Johnson, A.

    1988-01-01

    BNFL is in a unique position in that it has commercial experience of diffusion enrichment, and of centrifuge enrichment through its associate company Urenco. In addition BNFL is developing laser enrichment techniques as part of a UK development programme in this area. The paper describes the development programme which led to the introduction of competitive centrifuge enrichment technology by Urenco and discusses the areas where improvements have and will continue to be made in the centrifuge process. It also describes the laser development programme currently being undertaken in the UK. The paper concludes by discussing the relative merits of the various methods of uranium enrichment, with particular reference to the enrichment market likely to obtain over the rest of the century

  20. Advanced enrichment techniques

    International Nuclear Information System (INIS)

    Johnson, A.

    1987-01-01

    BNFL is in a unique position in that it has commercial experience of diffusion enrichment, and of centrifuge enrichment through its associate company Urenco. In addition BNFL is developing laser enrichment techniques as part of a UK development programme in this area. The paper describes the development programme which led to the introduction of competitive centrifuge enrichment technology by Urenco and discusses the areas where improvements have and will continue to be made in the centrifuge process. It also describes the laser development programme currently being undertaken in the UK. The paper concludes by discussing the relative merits of the various methods of uranium enrichment, with particular reference to the enrichment market likely to obtain over the rest of the century. (author)

  1. Measurement of nitrogen fixation in beam (Phaseolus vulgaris L.) cv. carioca, using a 15N2 low enrichment method

    International Nuclear Information System (INIS)

    Trivelin, P.C.O.; Matsui, E.; Saito, S.M.T.; Libardi, P.L.; Salati, E.

    1984-01-01

    A experimental work under field conditions to develop a method to measure atmospheric N 2 -fixation by leguminous plants, using a low enrichment 15 N 2 technique, is carried out. The experiment was developed using a N 2 -fixation measuring chamber on Terra Roxa Estruturada. The beam plants had their aereal part under normal conditions and the rooting system confined, through which a mixture of Ar, O 2 and N 2 labelled with 15 N (1.9% atom excess) was circulated from the 22nd to the 31st day from planting. Samples of the gaseous Ar, O 2 and N 2 mixture were analysed by mass spectrometry to determine 15 N concentrations and O 2 and CO 2 contents. The N 2 -fixed was measured by determination of total-N and isotopic concentration of nitrogen in the plants. (M.A.C.) [pt

  2. Implementation of preference ranking organization method for enrichment evaluation (Promethee) on selection system of student’s achievement

    Science.gov (United States)

    Karlitasari, L.; Suhartini, D.; Nurrosikawati, L.

    2018-03-01

    Selection of Student Achievement is conducted every year, starting from the level of Study Program, Faculty, to University, which then rank one will be sent to Kopertis level. The criteria made for the selection are Academic and Rich Scientific, Organizational, Personality, and English. In order for the selection of Student Achievement is Objective, then in addition to the presence of the jury is expected to use methods that support the decision to be more optimal in determining the Student Achievement. One method used is the Promethee Method. Preference Ranking Organization Method for Enrichment Evaluation (Promethee) is a method of ranking in Multi Criteria Decision Making (MCDM). PROMETHEE has the advantage that there is a preference type against the criteria that can take into account alternatives with other alternatives on the same criteria. The conjecture of alternate dominance over a criterion used in PROMETHEE is the use of values in the relationships between alternative ranking values. Based on the calculation result, from 7 applicants between Manual and Promethee Matrices, rank 1, 2, and 3, did not change, only 4 to 7 positions were changed. However, after the sensitivity test, almost all criteria experience a high level of sensitivity. Although it does not affect the students who will be sent to the next level, but can bring psychological impact on prospective student’s achievement

  3. Development of gel-filter method for high enrichment of low-molecular weight proteins from serum.

    Directory of Open Access Journals (Sweden)

    Lingsheng Chen

    Full Text Available The human serum proteome has been extensively screened for biomarkers. However, the large dynamic range of protein concentrations in serum and the presence of highly abundant and large molecular weight proteins, make identification and detection changes in the amount of low-molecular weight proteins (LMW, molecular weight ≤ 30kDa difficult. Here, we developed a gel-filter method including four layers of different concentration of tricine SDS-PAGE-based gels to block high-molecular weight proteins and enrich LMW proteins. By utilizing this method, we identified 1,576 proteins (n = 2 from 10 μL serum. Among them, 559 (n = 2 proteins belonged to LMW proteins. Furthermore, this gel-filter method could identify 67.4% and 39.8% more LMW proteins than that in representative methods of glycine SDS-PAGE and optimized-DS, respectively. By utilizing SILAC-AQUA approach with labeled recombinant protein as internal standard, the recovery rate for GST spiked in serum during the treatment of gel-filter, optimized-DS, and ProteoMiner was 33.1 ± 0.01%, 18.7 ± 0.01% and 9.6 ± 0.03%, respectively. These results demonstrate that the gel-filter method offers a rapid, highly reproducible and efficient approach for screening biomarkers from serum through proteomic analyses.

  4. Method Development to Increase Protein Enrichment During Dry Fractionation of Starch-Rich Legumes

    NARCIS (Netherlands)

    Pelgrom, P.J.M.; Boom, R.M.; Schutyser, M.A.I.

    2015-01-01

    A facile method was developed to establish milling settings that optimally separate starch granules from protein bodies and cell wall fibres for starch-rich legumes. Optimal separation was obtained for pea, bean, lentil and chickpea when the particle size distribution curve of flour and isolated

  5. A Facile Method for Separating and Enriching Nano and Submicron Particles from Titanium Dioxide Found in Food and Pharmaceutical Products.

    Science.gov (United States)

    Faust, James J; Doudrick, Kyle; Yang, Yu; Capco, David G; Westerhoff, Paul

    2016-01-01

    Recent studies indicate the presence of nano-scale titanium dioxide (TiO2) as an additive in human foodstuffs, but a practical protocol to isolate and separate nano-fractions from soluble foodstuffs as a source of material remains elusive. As such, we developed a method for separating the nano and submicron fractions found in commercial-grade TiO2 (E171) and E171 extracted from soluble foodstuffs and pharmaceutical products (e.g., chewing gum, pain reliever, and allergy medicine). Primary particle analysis of commercial-grade E171 indicated that 54% of particles were nano-sized (i.e., E171 and E171 isolated from foodstuffs and pharmaceuticals was accomplished using rate-zonal centrifugation. Commercial-grade E171 was separated into nano- and submicron-enriched fractions consisting of a nano:submicron fraction of approximately 0.45:1 and 3.2:1, respectively. E171 extracted from gum had nano:submicron fractions of 1.4:1 and 0.19:1 for nano- and submicron-enriched, respectively. We show a difference in particle adhesion to the cell surface, which was found to be dependent on particle size and epithelial orientation. Finally, we provide evidence that E171 particles are not immediately cytotoxic to the Caco-2 human intestinal epithelium model. These data suggest that this separation method is appropriate for studies interested in isolating the nano-sized particle fraction taken directly from consumer products, in order to study separately the effects of nano and submicron particles.

  6. A Comparison of Platelet Count and Enrichment Percentages in the Platelet Rich Plasma (PRP) Obtained Following Preparation by Three Different Methods.

    Science.gov (United States)

    Sabarish, Ram; Lavu, Vamsi; Rao, Suresh Ranga

    2015-02-01

    Platelet rich plasma (PRP) represents an easily accessible and rich source of autologous growth factors. Different manual methods for the preparation of PRP have been suggested. Lacuna in knowledge exists about the efficacy of PRP preparation by these different manual methods. This study was performed to determine the effects of centrifugation rate revolutions per minute (RPM) and time on the platelet count and enrichment percentages in the concentrates obtained following the three different manual methods of PRP preparation. In vitro experimental study. This was an experimental study in which platelet concentration was assessed in the PRP prepared by three different protocols as suggested by Marx R (method 1), Okuda K (method 2) and Landesberg R (method 3). A total of 60 peripheral blood samples, (n=20 per method) were obtained from healthy volunteers. Baseline platelet count was assessed for all the subjects following which PRP was prepared. The platelet count in the PRP was determined using coulter counter (Sysmex XT 2000i). The mean of the platelet count obtained and their enrichment percentage were calculated and intergroup comparison was done (Tukey's HSD test). The number of platelets and enrichment percentage in PRP prepared by method 1 was higher compared to method 2 and method 3; this difference in platelet concentrates was found to be statistically significant (p < 0.05). The centrifugation rate and time appear to be important parameters, which influence the platelet yield. Method 1 which had lower centrifugation rate and time yielded a greater platelet count and enrichment percentage.

  7. Uranium enrichment

    International Nuclear Information System (INIS)

    1990-01-01

    This report looks at the following issues: How much Soviet uranium ore and enriched uranium are imported into the United States and what is the extent to which utilities flag swap to disguise these purchases? What are the U.S.S.R.'s enriched uranium trading practices? To what extent are utilities required to return used fuel to the Soviet Union as part of the enriched uranium sales agreement? Why have U.S. utilities ended their contracts to buy enrichment services from DOE?

  8. Method for processing coal-enrichment waste with solid and volatile fuel inclusions

    Science.gov (United States)

    Khasanova, A. V.; Zhirgalova, T. B.; Osintsev, K. V.

    2017-10-01

    The method relates to the field of industrial heat and power engineering. It can be used in coal preparation plants for processing coal waste. This new way is realized to produce a loose ash residue directed to the production of silicate products and fuel gas in rotary kilns. The proposed method is associated with industrial processing of brown coal beneficiation waste. Waste is obtained by flotation separation of rock particles up to 13 mm in size from coal particles. They have in their composition both solid and volatile fuel inclusions (components). Due to the high humidity and significant rock content, low heat of combustion, these wastes are not used on energy boilers, they are stored in dumps polluting the environment.

  9. Enrichment Method for the Isolation of Bioactive Actinomycetes From Mangrove Sediments of Andaman Islands, India

    Directory of Open Access Journals (Sweden)

    Baskaran, R.

    2011-01-01

    Full Text Available Various pre-treatment methods and three different media were employed for the isolation of bioactive actinomycetes from mangrove sediments of Andaman and Nicobar Islands, India. Sediments from four different sites of mangrove forest were collected and pre-treated by dry heat method, and the media were supplemented with cycloheximide 80 µg/mL and nalidixic acid 75 µg/mL. The mean actinomycetes population density in sediment samples were recorded as 22 CFU-10^-6/gm in KUA medium followed by 12 CFU-10^-6/gm in AIA medium and 8 CFU-10^-6/gm in SCA medium. A total of 42 actinomycetes were isolated, and all the isolates were evaluated for their antibacterial activity against pathogenic bacteria on two different media. Among 42 isolates tested, 22 species were found to be antibacterial metabolite producer against test bacteria namely, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi and Klebsiella pneumoniae. Particularly, the actinomycete strains such as A101, A102, A107, A116, A121, A125, A130, F101, F102, F104, F106, De101 and De102 significantly inhibited the growth of all bacteria which were tested. Of these strains, A107 was identified as Streptomyces spp. This strain had the maximum activity against all used pathogens on both medium. Hence, the isolation, characterization and studies of secondary metabolites of actinomycetes from mangrove sediments in Andaman and Nicobar Island could be a pathway for discovery of antibiotics from marine actinomycetes.

  10. Uranium enrichment

    International Nuclear Information System (INIS)

    1989-01-01

    GAO was asked to address several questions concerning a number of proposed uranium enrichment bills introduced during the 100th Congress. The bill would have restructured the Department of Energy's uranium enrichment program as a government corporation to allow it to compete more effectively in the domestic and international markets. Some of GAO's findings discussed are: uranium market experts believe and existing market models show that the proposed DOE purchase of a $750 million of uranium from domestic producers may not significantly increase production because of large producer-held inventories; excess uranium enrichment production capacity exists throughout the world; therefore, foreign producers are expected to compete heavily in the United States throughout the 1990s as utilities' contracts with DOE expire; and according to a 1988 agreement between DOE's Offices of Nuclear Energy and Defense Programs, enrichment decommissioning costs, estimated to total $3.6 billion for planning purposes, will be shared by the commercial enrichment program and the government

  11. Uranium enrichment

    International Nuclear Information System (INIS)

    Rae, H.K.; Melvin, J.G.

    1988-06-01

    Canada is the world's largest producer and exporter of uranium, most of which is enriched elsewhere for use as fuel in LWRs. The feasibility of a Canadian uranium-enrichment enterprise is therefore a perennial question. Recent developments in uranium-enrichment technology, and their likely impacts on separative work supply and demand, suggest an opportunity window for Canadian entry into this international market. The Canadian opportunity results from three particular impacts of the new technologies: 1) the bulk of the world's uranium-enrichment capacity is in gaseous diffusion plants which, because of their large requirements for electricity (more than 2000 kW·h per SWU), are vulnerable to competition from the new processes; 2) the decline in enrichment costs increases the economic incentive for the use of slightly-enriched uranium (SEU) fuel in CANDU reactors, thus creating a potential Canadian market; and 3) the new processes allow economic operation on a much smaller scale, which drastically reduces the investment required for market entry and is comparable with the potential Canadian SEU requirement. The opportunity is not open-ended. By the end of the century the enrichment supply industry will have adapted to the new processes and long-term customer/supplier relationships will have been established. In order to seize the opportunity, Canada must become a credible supplier during this century

  12. Uranium enrichment

    International Nuclear Information System (INIS)

    Mohrhauer, H.

    1982-01-01

    The separation of uranium isotopes in order to enrich the fuel for light water reactors with the light isotope U-235 is an important part of the nuclear fuel cycle. After the basic principals of isotope separation the gaseous diffusion and the centrifuge process are explained. Both these techniques are employed on an industrial scale. In addition a short review is given on other enrichment techniques which have been demonstrated at least on a laboratory scale. After some remarks on the present situation on the enrichment market the progress in the development and the industrial exploitation of the gas centrifuge process by the trinational Urenco-Centec organisation is presented. (orig.)

  13. Radiometric enrichment of nonradioactive ores

    International Nuclear Information System (INIS)

    Mokrousov, V.A.; Lileev, V.A.

    1979-01-01

    Considered are the methods of mineral enrichment based on the use of the radioation of various types. The physical essence of enrichment processes is presented, their classification is given. Described are the ore properties influencing the efficiency of radiometric enrichment, methods of the properties study and estimation of ore enrichment. New possibilities opened by radiometric enrichment in the technology of primary processing of mineral raw materials are elucidated. A considerable attention is paid to the main and auxiliary equipment for radiometric enrichment. The foundations of the safety engineering are presented in a brief form. Presented are also results of investigations and practical works in the field of enrichment of ores of non-ferrous, ferrous and non-metallic minerals with the help of radiometric methods

  14. New method for GC/FID and GC-C-IRMS analysis of plasma free fatty acid concentration and isotopic enrichment.

    Science.gov (United States)

    Kangani, Cyrous O; Kelley, David E; Delany, James P

    2008-09-15

    A simple, direct and accurate method for the determination of concentration and enrichment of free fatty acids (FFAs) in human plasma was developed. The validation and comparison to a conventional method are reported. Three amide derivatives, dimethyl, diethyl and pyrrolidide, were investigated in order to achieve optimal resolution of the individual fatty acids. This method involves the use of dimethylamine/Deoxo-Fluor to derivatize plasma free fatty acids to their dimethylamides. This derivatization method is very mild and efficient, and is selective only towards FFAs so that no separation from a total lipid extract is required. The direct method gave lower concentrations for palmitic acid and stearic acid and increased concentrations for oleic acid and linoleic acid in plasma as compared to methyl ester derivative after thin-layer chromatography. The [(13)C]palmitate isotope enrichment measured using direct method was significantly higher than that observed with the BF(3)/MeOH-TLC method. The present method provided accurate and precise measures of concentration as well as enrichment when analyzed with gas chromatography combustion-isotope ratio-mass spectrometry.

  15. VladBot. Robot para posicionamiento de medida

    OpenAIRE

    Plata Rueda, Ángel

    2013-01-01

    VladBot es un robot autónomo diseñado para posicionar en interiores un micrófono de medida. Este prototipo puede valorar la idea de automatizar medidas acústicas en interiores mediante un robot autónomo. Posee dos ruedas motrices y una rueda loca. Ésta rueda loca aporta maniobrabilidad al robot. Un soporte extensible hecho de aluminio sostiene el micrófono de medida. VladBot ha sido diseñado con tecnologías de bajo coste y bajo una plataforma abierta, Arduino. Arduino es una plataforma el...

  16. Multiplex enrichment quantitative PCR (ME-qPCR): a high-throughput, highly sensitive detection method for GMO identification.

    Science.gov (United States)

    Fu, Wei; Zhu, Pengyu; Wei, Shuang; Zhixin, Du; Wang, Chenguang; Wu, Xiyang; Li, Feiwu; Zhu, Shuifang

    2017-04-01

    Among all of the high-throughput detection methods, PCR-based methodologies are regarded as the most cost-efficient and feasible methodologies compared with the next-generation sequencing or ChIP-based methods. However, the PCR-based methods can only achieve multiplex detection up to 15-plex due to limitations imposed by the multiplex primer interactions. The detection throughput cannot meet the demands of high-throughput detection, such as SNP or gene expression analysis. Therefore, in our study, we have developed a new high-throughput PCR-based detection method, multiplex enrichment quantitative PCR (ME-qPCR), which is a combination of qPCR and nested PCR. The GMO content detection results in our study showed that ME-qPCR could achieve high-throughput detection up to 26-plex. Compared to the original qPCR, the Ct values of ME-qPCR were lower for the same group, which showed that ME-qPCR sensitivity is higher than the original qPCR. The absolute limit of detection for ME-qPCR could achieve levels as low as a single copy of the plant genome. Moreover, the specificity results showed that no cross-amplification occurred for irrelevant GMO events. After evaluation of all of the parameters, a practical evaluation was performed with different foods. The more stable amplification results, compared to qPCR, showed that ME-qPCR was suitable for GMO detection in foods. In conclusion, ME-qPCR achieved sensitive, high-throughput GMO detection in complex substrates, such as crops or food samples. In the future, ME-qPCR-based GMO content identification may positively impact SNP analysis or multiplex gene expression of food or agricultural samples. Graphical abstract For the first-step amplification, four primers (A, B, C, and D) have been added into the reaction volume. In this manner, four kinds of amplicons have been generated. All of these four amplicons could be regarded as the target of second-step PCR. For the second-step amplification, three parallels have been taken for

  17. Radioimmunoassay method for measurement of plasma androstenedione. Its validation in ovulatory women and in patients with polycystic ovarian syndrome; Metodo de radioimunoensaio para medida da androstenediona plasmatica. Validacao em mulheres ovulatorias e com sindrome dos ovarios policisticos

    Energy Technology Data Exchange (ETDEWEB)

    Vilanova Socorro Veras, Maria do; Silva e Rosa, Alzira Amelia; Moura, Marcos Dias de; Ferriano, Rui Alberto; Sa, Marcos Felipe Silva de [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Medicina

    1995-01-01

    The present paper has as objective the standardization of a radioimmunoassay method for measurement of androstenedione. Ethyl ether was used for plasma extraction. The sensitivity of the method was 6,8 pg/tube; the reproducibility (inter assay error) was 15,6%; the precision (intrassay error) was 5,2%. As biological control, 20 ovulatory women showed median plasma values of 1250 pg/ml and 24 women with polycystic ovary syndrome presented median plasma values of 2.037 pg/ml. (author). 6 refs., 2 figs., 1 tab.

  18. Analytical method of Kr-85 determination, using cryogenic concentration and separation and liquid scintillation counting; Desarrollo del metodo de concentracion y se paracion criogenica cromatografica y medida radiactiva por centelleo liquido de Kr-85

    Energy Technology Data Exchange (ETDEWEB)

    Heras, M C; Perez, M M; Grau, A

    1983-07-01

    The method used in the Laboratory of the JEN for the determination of Kr-85 levels in gaseous effluents of nuclear power and in the atmosphere is described. Samples of air, collected in metallic cylinders, are introduced into a gas-solid chromatographic separation system which resolves Kr from the other air components. The separated Kr ia dissolved in a toluene based scintillation cocktail, and the Kr-85 content is determined by liquid scintillation counting. (Author)

  19. Correlation between macro texture measures carried out by the volumetric method and by different laser texture meter; Correlacion de medidas de macrotextura tomadas con el metodo volumetrico y con diferentes texturometros lasar

    Energy Technology Data Exchange (ETDEWEB)

    Parra Ruiz, L.; Yanguas Gonzalez, S. J.

    2013-06-01

    The reference value for the measurement of surface macro texture in the Spanish Main Road Network is the MTD or Mean Texture Depth (PMT, Profundidad Media de Textura), obtained by means of the volumetric methods, in accordance with the standard UNE EN 13036-1:12010. The fact that it is a spot measurement that requires road closures makes it an expensive procedure as well as slow and dangerous. In addition to this, the test results are relatively sensitive to the operator, being the procedure not too suitable for systematic surveys. These are some of the reasons that have contributed to the development of texture meter laser devices that can be assembled on board of vehicles, circulating without interfering with the normal traffic flow and providing a parameter named the MPD (Mean Depth Profile). According to the standard UNE-EN ISO 13473-1:2006, it is possible to estimate the texture obtained by volumetric methods, with the parameter ETD (Estimated Texture Depth) through the equation: ETD=0,8 x MPD+0.2 In 2008 CEDEX conducted a study that correlated macro texture measures obtained by means of the volumetric method with such carried out by different laser texture meters. The equations yield a better relation between MPD and MTD were dependent on the measurement device used and were not linear equations type, as is it indicated in the standard, but exponential type equations. (Author) 6 refs.

  20. Medida de similitud basada en saliencia

    Directory of Open Access Journals (Sweden)

    Sergio Domínguez

    2012-10-01

    quantifying saliency of feature values, for combining them during a comparison process and, eventually, to weight that feature attending to the result of the combination, are introduced as well. The results for the evaluation of this similarity measure in an image based content retrieval task are presented, as well as their comparison with those obtained using euclidean distance in the same task. Both are validated by volunteers who labelled the retrieved sets. Palabras clave: Bases de datos de imágenes, recuperación basada en contenido, medidas de similitud, modelos perceptuales, análisis de imágenes, Keywords: Image databases, content based retrieval, similarity measures, perceptual models, image analysis

  1. Comparison of PCR-Based Diagnosis with Centrifuged-Based Enrichment Method for Detection of Borrelia persica in Animal Blood Samples.

    Science.gov (United States)

    Naddaf, S R; Kishdehi, M; Siavashi, Mr

    2011-01-01

    The mainstay of diagnosis of relapsing fever (RF) is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by microscopy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spirochetemia. Animal blood samples with low degrees of spirochetemia were tested with two PCRs and a nested PCR targeting flaB, GlpQ, and rrs genes. Also, a centrifuged-based enrichment method and Giemsa staining were performed on blood samples with various degrees of spirochetemia. The flaB-PCR and nested rrs-PCR turned positive with various degrees of spirochetemia including the blood samples that turned negative with dark-field microscopy. The GlpQ-PCR was positive as far as at least one spirochete was seen in 5-10 microscopic fields. The sensitivity of GlpQ-PCR increased when DNA from Buffy Coat Layer (BCL) was used as template. The centrifuged-based enrichment method turned positive with as low concentration as 50 bacteria/ml blood, while Giemsa thick staining detected bacteria with concentrations ≥ 25000 bacteria/ml. Centrifuged-based enrichment method appeared as much as 500-fold more sensitive than thick smears, which makes it even superior to some PCR assays. Due to simplicity and minimal laboratory requirements, this method can be considered a valuable tool for diagnosis of RF in rural health centers.

  2. Comparison of direct-plating and broth-enrichment culture methods for detection of potential bacterial pathogens in respiratory secretions.

    Science.gov (United States)

    Kaur, Ravinder; Wischmeyer, Jareth; Morris, Matthew; Pichichero, Michael E

    2017-11-01

    We compared the recovery of potential respiratory bacterial pathogens and normal flora from nasopharyngeal specimens collected from children during health and at the onset of acute otitis media (AOM) by selective direct-plating and overnight broth-enrichment. Overall, 3442 nasal wash (NW) samples collected from young children were analysed from a 10-year prospective study. NWs were cultured by (1) direct-plating to TSAII/5 % sheep blood agar and chocolate agar plates and (2) overnight broth-enrichment in BacT/ALERT SA-broth followed by plating. Standard microbiology techniques were applied to identify three dominant respiratory bacterial pathogens: Streptococcus pneumoniae (Spn), Haemophilus influenzae (Hflu) and Moraxella catarrhalis (Mcat) as well as two common nasal flora, Staphylococcus aureus (SA) and alpha-haemolytic Streptococci (AHS).Results/Key findings. Direct-plating of NW resulted in isolation of Spn from 37.8 %, Hflu from 13.6 % and Mcat from 33.2 % of samples. In comparison, overnight broth-enrichment isolated fewer Spn (30.1 %), Hflu (6.2 %) and Mcat (16.2 %) (Penrichment resulted in significant increased isolation of SA (6.0 %) and AHS (30.1 %) (Penrichment when samples were collected from healthy children but not during AOM. In middle ear fluids (MEF) at the onset of AOM, broth-enrichment resulted in higher recovery of Spn (+10.4 %, Penrichment significantly reduces the accurate detection of bacterial respiratory pathogens and increases identification of SA and AHS in NW. Broth-enrichment improves detection of bacterial respiratory pathogens in MEF samples.

  3. Calibration method for a in vivo measurement system using mathematical simulation of the radiation source and the detector; Metodo de calibracao de um sistema de medida in vivo atraves da simulacao matematica da fonte de radiacao e do detector

    Energy Technology Data Exchange (ETDEWEB)

    Hunt, John

    1998-12-31

    A Monte Carlo program which uses a voxel phantom has been developed to simulate in vivo measurement systems for calibration purposes. The calibration method presented here employs a mathematical phantom, produced in the form of volume elements (voxels), obtained through Magnetic Resonance Images of the human body. The calibration method uses the Monte Carlo technique to simulate the tissue contamination, the transport of the photons through the tissues and the detection of the radiation. The program simulates the transport and detection of photons between 0.035 and 2 MeV and uses, for the body representation, a voxel phantom with a format of 871 slices each of 277 x 148 picture elements. The Monte Carlo code was applied to the calibration of in vivo systems and to estimate differences in counting efficiencies between homogeneous and non-homogeneous radionuclide distributions in the lung. Calculations show a factor of 20 between deposition of {sup 241} Am at the back compared with the front of the lung. The program was also used to estimate the {sup 137} Cs body burden of an internally contaminated individual, counted with an 8 x 4 Nal (TI) detector and an {sup 241} Am body burden of an internally contaminated individual, who was counted using a planar germanium detector. (author) 24 refs., 38 figs., 23 tabs.

  4. Moisture profile measurements of concrete samples in vertical flow by gamma ray attenuation method. Medidas do perfil de umidade de amostras de concreto em infiltracao vertical, atraves da atenuacao de raios gama

    Energy Technology Data Exchange (ETDEWEB)

    Appoloni, C R; Nardocci, A C; Obuti, M M [Universidade Estadual de Londrina, PR (Brazil). Dept. de Fisica

    1988-04-01

    This work deals with the study of the water diffusion in concrete by the gamma ray attenuation method. The moisture profiles, [theta] (z,t), of the vertical water flow were determined in concrete samples of different trace and porosity. The data were taken with a vertical and horizontal measurement table, a [sup 60] Co gamma ray source, a NaI (T) scintillation detector and the standard gamma ray spectrometry electronic. The [theta] (z,t) data analysis is presented using a phenomenological model of the moisture profile temporal evolution in heterogeneous materials. Two other models, Cell and Sandwich, were also applied to determine the attenuation coefficient of a non-homogeneous media from the attenuation coefficients of the components, taking into account particles-size effects. (author).

  5. Uranium enrichment

    International Nuclear Information System (INIS)

    1991-08-01

    This paper reports that in 1990 the Department of Energy began a two-year project to illustrate the technical and economic feasibility of a new uranium enrichment technology-the atomic vapor laser isotope separation (AVLIS) process. GAO believes that completing the AVLIS demonstration project will provide valuable information about the technical viability and cost of building an AVLIS plant and will keep future plant construction options open. However, Congress should be aware that DOE still needs to adequately demonstrate AVLIS with full-scale equipment and develop convincing cost projects. Program activities, such as the plant-licensing process, that must be completed before a plant is built, could take many years. Further, an updated and expanded uranium enrichment analysis will be needed before any decision is made about building an AVLIS plant. GAO, which has long supported legislation that would restructure DOE's uranium enrichment program as a government corporation, encourages DOE's goal of transferring AVLIS to the corporation. This could reduce the government's financial risk and help ensure that the decision to build an AVLIS plant is based on commercial concerns. DOE, however, has no alternative plans should the government corporation not be formed. Further, by curtailing a planned public access program, which would have given private firms an opportunity to learn about the technology during the demonstration project, DOE may limit its ability to transfer AVLIS to the private sector

  6. Multiplexed Single Intact Cell Droplet Digital PCR (MuSIC ddPCR) Method for Specific Detection of Enterohemorrhagic E. coli (EHEC) in Food Enrichment Cultures

    OpenAIRE

    McMahon, Tanis C.; Blais, Burton W.; Wong, Alex; Carrillo, Catherine D.

    2017-01-01

    Foodborne illness attributed to enterohemorrhagic E. coli (EHEC), a highly pathogenic subset of Shiga toxin-producing E. coli (STEC), is increasingly recognized as a significant public health issue. Current microbiological methods for identification of EHEC in foods often use PCR-based approaches to screen enrichment broth cultures for characteristic gene markers [i.e., Shiga toxin (stx) and intimin (eae)]. However, false positives arise when complex food matrices, such as beef, contain mixtu...

  7. Application of the A/E pulse shape discrimination method to first Ge-76 enriched BEGe detectors operated in GERDA

    Energy Technology Data Exchange (ETDEWEB)

    Lazzaro, Andrea; Agostini, Matteo; Budjas, Dusan; Schoenert, Stefan [Physik-Department E15, Technische Universitaet Muenchen (Germany); Collaboration: GERDA-Collaboration

    2013-07-01

    In 2013 the Gerda experiment will be upgraded to its second phase with more than double of the current {sup 76}Ge mass. The additional diodes are custom made Broad Energy Germanium (BEGe) detectors. This design has been chosen to enhance the pulse shape discrimination (PSD) capability, with respect to the Phase I coaxial detectors. The goal of Phase II is to improve by one order of magnitude the current background index; the PSD will bring a major contribution to this result. Since summer 2012 the first set of five enriched BEGe detectors are operated in Gerda Phase I. This offers us the possibility to test the PSD performances and the signal analysis in an environment as close as possible to the Gerda Phase II configuration. In this talk I present the A/E analysis, the calibration of the cut parameters and the results in terms of background reduction for the data taken with these enriched BEGe.

  8. Method for enriching and separating heavy hydrogen isotopes from substance streams containing such isotopes by means of isotope exchange

    International Nuclear Information System (INIS)

    Knochel, A.; Eggers, I.; Klatte, B.; Wilken, R. D.

    1985-01-01

    A process for enriching and separating heavy hydrogen isotopes having a heavy hydrogen cation (deuterium and/or tritium) from substance streams containing them, wherein the respectively present hydrogen isotopes are exchanged in chemical equilibria. A protic, acid solution containing deuterium and/or tritium is brought into contact with a value material from the group of open-chained polyethers or aminopolyethers, macro-monocyclic or macro-polycyclic polyethers, macro-monocyclic or macro-polycyclic amino polyethers, and mixtures of these values, in their free or proton salt form to form a reaction product of the heavy hydrogen cation with the value or value salt and bring about enrichment of deuterium and/or tritium in the reaction product. The reaction product containing the value or value salt is separated from the solution. The separated reaction product is treated to release the hydrogen isotope(s) to be enriched in the form of deuterium oxide (HDO) and/or tritium oxide (HTO) by regenerating the value or its salt, respectively. The regenerated value is returned for reuse

  9. Blueprint for domestic uranium enrichment

    International Nuclear Information System (INIS)

    1981-01-01

    The AEC advisory committee on domestic production of uranium enrichment has studied for more than a year how to achieve the domestic enrichment of uranium by the construction and operation of a commercial enriching plant using centrifugal separation method, and the report was submitted to the Atomic Energy Commission on August 18, 1980. Japan has depended wholly on overseas services for her uranium enrichment needs, but the development of domestic enrichment has been carried on in parallel. The AEC decided to construct a uranium enrichment pilot plant using centrifuges, and it has been forwarded as a national project. The plant is operated by the Power Reactor and Nuclear Fuel Development Corp. since 1979. The capacity of the plant will be raised to approximately 75 ton SWU a year. The centrifuges already operated have provided the first delivery of fuel of about 1 ton for the ATR ''Fugen''. The demand-supply balance of uranium enrichment service, the significance of the domestic enrichment of uranium, the evaluation of uranium enrichment technology, the target for domestic enrichment plan, the measures to promote domestic uranium enrichment, and the promotion of the construction of a demonstration plant are reported. (Kako, I.)

  10. Contemporary technology of enrichment of kaolins of Angren deposit. II. Removal of impurities and whitening of kaolins: using electrophysical and chemical methods of cleaning

    International Nuclear Information System (INIS)

    Krivorotov, V.F.; Usmanov, ZH.M.; Fridman, A.A.

    2012-01-01

    The contemporary methods of enrichment of Angren kaolins have been described. The electrophoresis and chemical whitening are demonstrated to be the most effective methods of scavenging. Application of ultrasonic dispersion, electrophoresis and chemical whitening methods allows one to obtain kaolin with whiteness ≅ 88% and content of iron oxides ≅ 0.4%. Using such kaolin as a sorbent at de coloration of plant oil have been shown good adsorption properties, meeting the level of world-wide standards for such class of materials. (authors)

  11. Effect of cooking method on the fatty acid content of reduced-fat and PUFA-enriched pork patties formulated with a konjac-based oil bulking system.

    Science.gov (United States)

    Salcedo-Sandoval, Lorena; Cofrades, Susana; Ruiz-Capillas, Claudia; Jiménez-Colmenero, Francisco

    2014-12-01

    The effect of cooking methods (electric grilling and pan-frying in olive oil) on the composition of reduced-fat and reduced-fat/PUFA enriched pork patties was studied. Fat reduction was performed by replacing pork backfat (38% and 100%) with konjac gel and PUFA-enrichment by replacing pork backfat (49%) with a konjac-based oil bulking system stabilizing a healthier oil combination (olive, linseed and fish oils). Cooking losses (13%-27%) were affected (pcooking procedure. Compared with raw products, cooked samples had higher (pcooked, the PUFA levels in the medium-fat/improved sample containing the oil bulking system ranged between 1.4 and 1.6g/100g (0.47-0.51 from n-3 PUFAs), with EPA+DHA concentrations of around 75mg/100g. Konjac materials were successfully used to produce pork patties with a better lipid composition. Copyright © 2014. Published by Elsevier Ltd.

  12. Multiplexed Single Intact Cell Droplet Digital PCR (MuSIC ddPCR) Method for Specific Detection of Enterohemorrhagic E. coli (EHEC) in Food Enrichment Cultures.

    Science.gov (United States)

    McMahon, Tanis C; Blais, Burton W; Wong, Alex; Carrillo, Catherine D

    2017-01-01

    Foodborne illness attributed to enterohemorrhagic E. coli (EHEC), a highly pathogenic subset of Shiga toxin-producing E. coli (STEC), is increasingly recognized as a significant public health issue. Current microbiological methods for identification of EHEC in foods often use PCR-based approaches to screen enrichment broth cultures for characteristic gene markers [i.e., Shiga toxin ( stx ) and intimin ( eae )]. However, false positives arise when complex food matrices, such as beef, contain mixtures of eae -negative STEC and eae -positive E. coli , but no EHEC with both markers in a single cell. To reduce false-positive detection of EHEC in food enrichment samples, a Multiplexed, Single Intact Cell droplet digital PCR (MuSIC ddPCR) assay capable of detecting the co-occurrence of the stx and eae genes in a single bacterial cell was developed. This method requires: (1) dispersal of intact bacteria into droplets; (2) release of genomic DNA (gDNA) by heat lysis; and (3) amplification and detection of genetic targets ( stx and eae ) using standard TaqMan chemistries with ddPCR. Performance of the method was tested with panels of EHEC and non-target E. coli . By determining the linkage (i.e., the proportion of droplets in which stx and eae targets were both amplified), samples containing EHEC (typically greater than 20% linkage) could be distinguished from samples containing mixtures of eae -negative STEC and eae -positive E. coli (0-2% linkage). The use of intact cells was necessary as this linkage was not observed with gDNA extracts. EHEC could be accurately identified in enrichment broth cultures containing excess amounts of background E. coli and in enrichment cultures derived from ground beef/pork and leafy-green produce samples. To our knowledge, this is the first report of dual-target detection in single bacterial cells using ddPCR. The application of MuSIC ddPCR to enrichment-culture screening would reduce false-positives, thereby improving the cost, speed, and

  13. Comparison of PCR-Based Diagnosis with Centrifuged-Based Enrichment Method for Detection of Borrelia Persica in Animal Blood Samples

    Directory of Open Access Journals (Sweden)

    SR Naddaf

    2011-06-01

    Full Text Available Background: The mainstay of diagnosis of relapsing fever (RF is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by mi­cros­copy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spi­ro­chetemia. Methods: Animal blood samples with low degrees of spirochetemia were tested with two PCRs and a nested PCR tar­get­ing flaB, GlpQ, and rrs genes. Also, a centrifuged-based enrichment method and Giemsa staining were per­formed on blood samples with various degrees of spirochetemia. Results: The flaB-PCR and nested rrs-PCR turned positive with various degrees of spirochetemia including the blood samples that turned negative with dark-field microscopy. The GlpQ-PCR was positive as far as at least one spi­ro­chete was seen in 5-10 microscopic fields. The sensitivity of GlpQ-PCR increased when DNA from Buffy Coat Layer (BCL was used as template. The centrifuged-based enrichment method turned positive with as low concentra­tion as 50 bacteria/ml blood, while Giemsa thick staining detected bacteria with concentrations ≥ 25000 bacteria/ml. Conclusion: Centrifuged-based enrichment method appeared as much as 500-fold more sensitive than thick smears, which makes it even superior to some PCR assays. Due to simplicity and minimal laboratory requirements, this method can be considered a valuable tool for diagnosis of RF in rural health centers.

  14. Comparison of PCR-Based Diagnosis with Centrifuged-Based Enrichment Method for Detection of Borrelia persica in Animal Blood Samples

    Directory of Open Access Journals (Sweden)

    SR Naddaf

    2011-06-01

    Background: The mainstay of diagnosis of relapsing fever (RF is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by mi­cros­copy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spi­ro­chetemia. Methods: Animal blood samples with low degrees of spirochetemia were tested with two PCRs and a nested PCR tar­get­ing flaB, GlpQ, and rrs genes. Also, a centrifuged-based enrichment method and Giemsa staining were per­formed on blood samples with various degrees of spirochetemia. Results: The flaB-PCR and nested rrs-PCR turned positive with various degrees of spirochetemia including the blood samples that turned negative with dark-field microscopy. The GlpQ-PCR was positive as far as at least one spi­ro­chete was seen in 5-10 microscopic fields. The sensitivity of GlpQ-PCR increased when DNA from Buffy Coat Layer (BCL was used as template. The centrifuged-based enrichment method turned positive with as low concentra­tion as 50 bacteria/ml blood, while Giemsa thick staining detected bacteria with concentrations ≥ 25000 bacteria/ml.  Conclusion: Centrifuged-based enrichment method appeared as much as 500-fold more sensitive than thick smears, which makes it even superior to some PCR assays. Due to simplicity and minimal laboratory requirements, this method can be considered a valuable tool for diagnosis of RF in rural health centers.  

  15. Thermal breeder fuel enrichment zoning

    International Nuclear Information System (INIS)

    Capossela, H.J.; Dwyer, J.R.; Luce, R.G.; McCoy, D.F.; Merriman, F.C.

    1992-01-01

    A method and apparatus for improving the performance of a thermal breeder reactor having regions of higher than average moderator concentration are disclosed. The fuel modules of the reactor core contain at least two different types of fuel elements, a high enrichment fuel element and a low enrichment fuel element. The two types of fuel elements are arranged in the fuel module with the low enrichment fuel elements located between the high moderator regions and the high enrichment fuel elements. Preferably, shim rods made of a fertile material are provided in selective regions for controlling the reactivity of the reactor by movement of the shim rods into and out of the reactor core. The moderation of neutrons adjacent the high enrichment fuel elements is preferably minimized as by reducing the spacing of the high enrichment fuel elements and/or using a moderator having a reduced moderating effect. 1 figure

  16. Uranium enrichment

    International Nuclear Information System (INIS)

    1991-11-01

    This paper analyzes under four different scenarios the adequacy of a $500 million annual deposit into a fund to pay for the cost of cleaning up the Department of Energy's (DOE) three aging uranium enrichment plants. These plants are located in Oak Ridge, Tennessee; Paducah, Kentucky; and Portsmouth, Ohio. In summary the following was found: A fixed annual $500 million deposit made into a cleanup fund would not be adequate to cover total expected cleanup costs, nor would it be adequate to cover expected decontamination and decommissioning (D and D) costs. A $500 million annual deposit indexed to an inflation rate would likely be adequate to pay for all expected cleanup costs, including D and D costs, remedial action, and depleted uranium costs

  17. Analysis of the energy efficiency of short rotation woody crops biomass as affected by different methods of soil enrichment

    International Nuclear Information System (INIS)

    Stolarski, Mariusz J.; Krzyżaniak, Michał; Tworkowski, Józef; Szczukowski, Stefan; Niksa, Dariusz

    2016-01-01

    The aim of this study was to determine the energy input and energy efficiency of the production of willow, poplar and black locust chips in four-year harvest rotation. The highest energy input was made in poplar production when soil was enriched with lignin and by mineral fertilisation (33.02 GJ ha −1 ). For willow production it was 30.76 GJ ha −1 when lignin, mycorrhiza and mineral fertilisation were used. The energy input in the production of black locust was much lower. The largest energy gain was obtained in the production of poplar when soil was enriched with lignin and mineral fertilisation (673.7 GJ ha −1 ). A similar level of this parameter (669.7 GJ ha −1 ) was achieved in the production of willow when lignin, mycorrhiza and mineral fertilisation was used. In general, a higher energy gain was obtained in the production of willow and poplar than in the production of black locust. On the other hand, the best energy efficiency ratio was achieved for willow (28.9) in the option with lignin. The ratio for poplar production ranged from 19.7 to 25.9. On the other hand, the energy efficiency ratio for black locust ranged from 10.6 to 21.7. - Highlights: • The energy input ranged from 6.69 GJ ha −1 to 33.02 GJ ha −1 . • The largest energy gain was obtained for poplar (673.7 GJ ha −1 ). • The best energy efficiency ratio was achieved for willow (28.9). • The energy efficiency ratio for poplar ranged from 19.7 to 25.9. • The energy efficiency ratio for black locust ranged from 10.6 to 21.7.

  18. Hacia una definición de la medida

    OpenAIRE

    Parot, Jean Jacques

    2012-01-01

    Hacia una definición de la medida A. Primera situación Un granjero va al mercado para vender a) Una gallina (20 pesos) b) Un bulto de naranjas (45 pesos) c) Un pate (38 pesos) d) Una docena de huevos (12 pesos)

  19. Gas-phase UF6 enrichment monitor for enrichment plant safeguards

    International Nuclear Information System (INIS)

    Strittmatter, R.B.; Tape, J.W.

    1980-03-01

    An in-line enrichment monitor is being developed to provide real-time enrichment data for the gas-phase UF 6 feed stream of an enrichment plant. The nondestructive gamma-ray assay method can be used to determine the enrichment of natural UF 6 with a relative precision of better than 1% for a wide range of pressures

  20. Determination of plant stanols and plant sterols in phytosterol enriched foods with a gas chromatographic-flame ionization detection method: NMKL collaborative study.

    Science.gov (United States)

    Laakso, Päivi H

    2014-01-01

    This collaborative study with nine participating laboratories was conducted to determine the total plant sterol and/or plant stanol contents in phytosterol fortified foods with a gas chromatographic method. Four practice and 12 test samples representing mainly commercially available foodstuffs were analyzed as known replicates. Twelve samples were enriched with phytosterols, whereas four samples contained only natural contents of phytosterols. The analytical procedure consisted of two alternative approaches: hot saponification method, and acid hydrolysis treatment prior to hot saponification. As a result, sterol/stanol compositions and contents in the samples were measured. The amounts of total plant sterols and total plant stanols varying from 0.005 to 8.04 g/100 g product were statistically evaluated after outliers were eliminated. The repeatability RSD (RSDr) varied from 1.34 to 17.13%. The reproducibility RSD (RSDR) ranged from 3.03 to 17.70%, with HorRat values ranging from 0.8 to 2.1. When only phytosterol enriched food test samples are considered, the RSDr ranged from 1.48 to 6.13%, the RSD, ranged from 3.03 to 7.74%, and HorRat values ranged from 0.8 to 2.1. Based on the results of this collaborative study, the study coordinator concludes the method is fit for its purpose.

  1. Pressure-assisted electrokinetic injection for on-line enrichment in capillary electrophoresis-mass spectrometry: a sensitive method for measurement of ten haloacetic acids in drinking water.

    Science.gov (United States)

    Zhang, Huijuan; Zhu, Jiping; Aranda-Rodriguez, Rocio; Feng, Yong-Lai

    2011-11-07

    Haloacetic acids (HAAs) are by-products of the chlorination of drinking water containing natural organic matter and bromide. A simple and sensitive method has been developed for determination of ten HAAs in drinking water. The pressure-assisted electrokinetic injection (PAEKI), an on-line enrichment technique, was employed to introduce the sample into a capillary electrophoresis (CE)-electrospray ionization-tandem mass spectrometry system (ESI-MS/MS). HAAs were monitored in selected reaction monitoring mode. With 3 min of PAEKI time, the ten major HAAs (HAA10) in drinking water were enriched up to 20,000-fold into the capillary without compromising resolution. A simple solid phase clean-up method has been developed to eliminate the influence of ionic matrices from drinking water on PAEKI. Under conditions optimized for mass spectrometry, PAEKI and capillary electrophoresis, detection limits defined as three times ratio of signal to noise have been achieved in a range of 0.013-0.12 μg L(-1) for ten HAAs in water sample. The overall recoveries for all ten HAAs in drinking water samples were between 76 and 125%. Six HAAs including monochloro- (MCAA), dichloro- (DCAA), trichloro- (TCAA), monobromo- (MBAA), bromochloro- (BCAA), and bromodichloroacetic acids (BDCAA) were found in tap water samples collected. Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.

  2. Estudo comparativo da medida da rotação vertebral pelos métodos de Nash & Moe e método de Raimondi Comparative study of the measurements of the vertebral rotation using Nash & Moe and Raimondi methods

    Directory of Open Access Journals (Sweden)

    Helton L. A. Defino

    2004-09-01

    Full Text Available Neste estudo foram avaliados a sensibilidade e precisão dos métodos de Nash e Moe e de Raimondi para a medida da rotação da vértebra torácica e lombar.Três cirurgiões de coluna avaliaram, independentemente, as radiografias de uma vértebra torácica (T9 e de uma vértebra lombar (L2 com graus de rotação que variaram de 0º a 60º e estabeleceram valores de acordo com o método de Nash e Moe e o método de Raimondi.Foram estudadas a concordância entre os examinadores para um determinado método, a variação das medidas obtidas na vértebra torácica e lombar a partir de uma mesma rotação real conhecida e a correlação entre um valor real conhecido de rotação vertebral e a sua estimativa pelos métodos utilizados no estudo . Os resultados mostraram boa concordância entre os examinadores para o método de Nash e Moe, tanto para a vértebra torácica (k médio = 0,66, quanto para a lombar (k médio = 0,80. Pelo método de Raimondi não houve diferença significativa entre os examinadores para a vértebra torácica, no entanto, para a vértebra lombar houve baixa reprodutibilidade do método.Para uma mesma rotação na vértebra torácica e lombar os resultados foram não concordantes pelo método de Nash e Moe, e pelo método de Raimondi os valores observados para a vértebra torácica foram significativamente maiores que os da vértebra lombar. A correlação entre os valores reais e as estimados pelo método de Raimondi para a vértebra torácica mostrou que houve diferença significativa produzida em função da rotação até 20º graus, já para a vértebra lombar, os valores obtidos foram muito próximos do real.The sensibility and precision of the Nash and Moe and Raimondi methods were evaluated in this study for the measurement of the rotation of the thoracic and the lumbar vertebra. Three spine surgeons evaluated, independently, the x-rays of a thoracic vertebra (T9 and of a lumbar vertebra (L2 with varying rotational

  3. Análise das medidas aerodinâmicas no português brasileiro por meio do Método Multiparamétrico de Avaliação Vocal Objetiva Assistida (EVA Analysis of aerodynamic measures in Brazilian portuguese through Multiparameter Assessment Method Vocal Objective Assist (EVA

    Directory of Open Access Journals (Sweden)

    Karina Vitor de Oliveira

    2012-01-01

    Full Text Available OBJETIVO: definir medidas aerodinâmicas em falantes do português brasileiro, sem queixas vocais, obtidas pelo programa EVA. MÉTODO: participaram do estudo 20 homens e 20 mulheres, que tiveram suas vozes analisadas. RESULTADOS: os valores médios referentes à pressão subglótica para vozes femininas e masculinas foram, respectivamente: Pressão subglótica= 5,84 hPa e 6,7 hPa, média da intensidade= 79,21 dB e 81,7 dB, média do fluxo oral= 0,09 dm³/s e 0,16 dm³/s, eficiência glótica= 13,87 dB/hPa e 12,78 dB/hPa, eficiência laríngea= 188,08 dB/(hPa.dm³/s e 97,11 dB/(hPa.dm³/s, resistência laríngea= 96,26 hPa/(dm³/s e 52,64 hPa/(dm³/s, média da frequência fundamental (F0= 208,28 Hz e 136,56 Hz e pico da pressão do fluxo oral= 0,093 e 0,098. Houve diferença estatisticamente significante em relação ao gênero para as medidas de média de intensidade, eficiência laríngea, resistência laríngea e média da frequência fundamental. Os valores médios referentes ao fluxo oral para vozes femininas e masculinas foram respectivamente: Pressão subglótica= 6,05 hPa e 6,6 hPa, média da intensidade= 65,50 dB e 66,3 dB, média do fluxo oral= 0,10 dm³/s e 0,13 dm³/s, eficiência glótica= 11,12 dB/hPa e 11,77 dB/hPa, eficiência laríngea= 144,83 dB/(hPa.dm³/s e 97,89 dB/(hPa.dm³/s, resistência laríngea= 78,98 hPa/(dm³/s e 61,81 hPa/(dm³/s, média da F0= 222,52 Hz e 139,20 Hz e pico da pressão do fluxo oral= 0,047 e 0,053. CONCLUSÃO: o EVA é um programa ainda novo no Brasil, e a análise de medidas aerodinâmicas, em falantes do português brasileiro, permite a obtenção de valores de referência, possibilitando assim comparações com estudos futuros.PURPOSE: to define aerodynamic measures in Brazilian Portuguese speakers without voice complaints, obtained by the EVA program. METHOD: the study included 20 men and 20 women who had their voices analyzed. RESULTS: the mean values with subglottic pressure for female and male

  4. Analysis of isotope element by electrolytic enrichment method for ground water and surface water in Saurashtra region, Gujarat, India

    Directory of Open Access Journals (Sweden)

    Sajal Singh

    2016-12-01

    Full Text Available The present study has been aimed for the assessment of isotope element Tritium (3H. It is a great threat to human health and environment for lengthy duration. The tritium exists in earth in diverse forms such as (1 small amounts of natural tritium are produced by alpha decay of lithium-7, (2 natural atmospheric tritium is also generated by secondary neutron cosmic ray bombardment of nitrogen, (3 atmospheric nuclear bomb testing in the 1950s, although the contribution from nuclear power plants is small. Tritium or 3H is a radioactive isotope of hydrogen with a half-life of 12.32 ± 0.02 years. Water samples from ground water, surface water, and precipitation were collected from different locations in Gujarat area and were analyzed for the same. Distillation of samples was done to reduce the conductivity. Deuterium and Hydrogen were removed by the process of physico-chemical fractionation in the tritium enrichment unit. The basis of physico-chemical fractionation is the difference in the strength of bonds formed by the light vs. the heavier isotope of a given element. A total of 10 cycles (runs were executed using Quintals process. Tritium concentration files were created with help of WinQ and Quick start software in Quintals process (Liquid Scintillation Spectrometer. The concentration of tritium in terms of tritium units (TU of various samples has been determined. The TU values of the samples vary in the range of 0.90–6.62 TU.

  5. The commercial role for centrifuge enrichment

    International Nuclear Information System (INIS)

    Readle, P.H.; Wilcox, P.

    1987-01-01

    The enrichment market is extremely competitive and capacity greatly exceeds demand. BNFL [British Nuclear Fuels Ltd.] is in a unique position in having commercial experience of the two enrichment technologies currently used industrially: diffusion, and centrifuge enrichment through its associate company Urenco. In addition, BNFL is developing laser enrichment techniques as part of a UK development programme. The paper describes the enrichment market, briefly discusses the relative merits of the various methods of uranium enrichment and concludes that the gas centrifuge will be best able to respond to market needs for at least the remainder of the century. (author)

  6. A novel method for sample preparation of fresh lung cancer tissue for proteomics analysis by tumor cell enrichment and removal of blood contaminants

    Directory of Open Access Journals (Sweden)

    Orre Lotta

    2010-02-01

    Full Text Available Abstract Background In-depth proteomics analyses of tumors are frequently biased by the presence of blood components and stromal contamination, which leads to large experimental variation and decreases the proteome coverage. We have established a reproducible method to prepare freshly collected lung tumors for proteomics analysis, aiming at tumor cell enrichment and reduction of plasma protein contamination. We obtained enriched tumor-cell suspensions (ETS from six lung cancer cases (two adenocarcinomas, two squamous-cell carcinomas, two large-cell carcinomas and from two normal lung samples. The cell content of resulting ETS was evaluated with immunocytological stainings and compared with the histologic pattern of the original specimens. By means of a quantitative mass spectrometry-based method we evaluated the reproducibility of the sample preparation protocol and we assessed the proteome coverage by comparing lysates from ETS samples with the direct lysate of corresponding fresh-frozen samples. Results Cytological analyses on cytospin specimens showed that the percentage of tumoral cells in the ETS samples ranged from 20% to 70%. In the normal lung samples the percentage of epithelial cells was less then 10%. The reproducibility of the sample preparation protocol was very good, with coefficient of variation at the peptide level and at the protein level of 13% and 7%, respectively. Proteomics analysis led to the identification of a significantly higher number of proteins in the ETS samples than in the FF samples (244 vs 109, respectively. Albumin and hemoglobin were among the top 5 most abundant proteins identified in the FF samples, showing a high contamination with blood and plasma proteins, whereas ubiquitin and the mitochondrial ATP synthase 5A1 where among the top 5 most abundant proteins in the ETS samples. Conclusion The method is feasible and reproducible. We could obtain a fair enrichment of cells but the major benefit of the method

  7. El trabajo en Smith: valor o medida del valor?

    Directory of Open Access Journals (Sweden)

    Adolfo Rodríguez Herrera

    2016-12-01

    Este artículo revisa los conceptos desarrollados por Smith para formular su teoría del valor (valor, precio real y valor de  cambio. Nuestra interpretación de sus textos sobre el valor no coincide con la que tradicionalmente se ha hecho. De acuerdo con nuestra interpretación, no sería correcta la crítica de Ricardo y Marx a la posición de Smith sobre el papel del trabajo como medida de valor. Para estos autores, Smith no es consistente al proponer que el valor de una mercancía es definido o medido como la cantidad de trabajo necesaria para producirla y simultáneamente como la cantidad de trabajo que puede ser comprada por esta mercancía. Tratamos de demostrar que para Smith el trabajo tiene un doble papel –como fuente y como medida del valor–, y que a ello se debe la confusión generada por su uso de algunos términos: Smith propone el trabajo como una medida de valor porque lo concibe como una fuente de valor. Con esta interpretación queda claro, paradójicamente, que la teoría del valor trabajo de Smith corresponde sustancialmente con la que más tarde desarrollarán Ricardo y Marx.

  8. Enriching Careers and Lives: Introducing a Positive, Holistic, and Narrative Career Counseling Method that Bridges Theory and Practice

    Science.gov (United States)

    Zikic, Jelena; Franklin, Mark

    2010-01-01

    CareerCycles (CC) career counseling framework and method of practice integrates and builds on aspects of positive psychology. Through its holistic and narrative approach, the CC method seeks to collaboratively identify and understand clients' career and life stories. It focuses on their strengths, desires, preferences, assets, future…

  9. Juvenile psittacine environmental enrichment.

    Science.gov (United States)

    Simone-Freilicher, Elisabeth; Rupley, Agnes E

    2015-05-01

    Environmental enrichment is of great import to the emotional, intellectual, and physical development of the juvenile psittacine and their success in the human home environment. Five major types of enrichment include social, occupational, physical, sensory, and nutritional. Occupational enrichment includes exercise and psychological enrichment. Physical enrichment includes the cage and accessories and the external home environment. Sensory enrichment may be visual, auditory, tactile, olfactory, or taste oriented. Nutritional enrichment includes variations in appearance, type, and frequency of diet, and treats, novelty, and foraging. Two phases of the preadult period deserve special enrichment considerations: the development of autonomy and puberty. Copyright © 2015 Elsevier Inc. All rights reserved.

  10. Proof of concept of a "greener" protein purification/enrichment method based on carboxylate-terminated carbosilane dendrimer-protein interactions.

    Science.gov (United States)

    González-García, Estefanía; Maly, Marek; de la Mata, Francisco Javier; Gómez, Rafael; Marina, María Luisa; García, María Concepción

    2016-11-01

    Protein sample preparation is a critical and an unsustainable step since it involves the use of tedious methods that usually require high amount of solvents. The development of new materials offers additional opportunities in protein sample preparation. This work explores, for the first time, the potential application of carboxylate-terminated carbosilane dendrimers to the purification/enrichment of proteins. Studies on dendrimer binding to proteins, based on protein fluorescence intensity and emission wavelengths measurements, demonstrated the interaction between carboxylate-terminated carbosilane dendrimers and proteins at all tested pH levels. Interactions were greatly affected by the protein itself, pH, and dendrimer concentration and generation. Especially interesting was the interaction at acidic pH since it resulted in a significant protein precipitation. Dendrimer-protein interactions were modeled observing stable complexes for all proteins. Carboxylate-terminated carbosilane dendrimers at acidic pH were successfully used in the purification/enrichment of proteins extracted from a complex sample. Graphical Abstract Images showing the growing turbidity of solutions containing a mixture of proteins (lysozyme, myoglobin, and BSA) at different protein:dendrimer ratios (1:0, 1:1, 1:8, and 1:20) at acidic pH and SDS-PAGE profiles of the corresponsing supernatants. Comparison of SDS-PAGE profiles for the pellets obtained during the purification of proteins present in a complex sample using a conventional "no-clean" method based on acetone precipitation and the proposed "greener" method using carboxylate-terminated carbosilane dendrimer at a 1:20 protein:dendrimer ratio.

  11. A novel membrane inlet mass spectrometer method to measure ¹⁵NH4₄⁺ for isotope-enrichment experiments in aquatic ecosystems.

    Science.gov (United States)

    Yin, Guoyu; Hou, Lijun; Liu, Min; Liu, Zhanfei; Gardner, Wayne S

    2014-08-19

    Nitrogen (N) pollution in aquatic ecosystems has attracted much attention over the past decades, but the dynamics of this bioreactive element are difficult to measure in aquatic oxygen-transition environments. Nitrogen-transformation experiments often require measurement of (15)N-ammonium ((15)NH4(+)) ratios in small-volume (15)N-enriched samples. Published methods to determine N isotope ratios of dissolved ammonium require large samples and/or costly equipment and effort. We present a novel ("OX/MIMS") method to determine N isotope ratios for (15)NH4(+) in experimental waters previously enriched with (15)N compounds. Dissolved reduced (15)N (dominated by (15)NH4(+)) is oxidized with hypobromite iodine to nitrogen gas ((29)N2 and/or (30)N2) and analyzed by membrane inlet mass spectrometry (MIMS) to quantify (15)NH4(+) concentrations. The N isotope ratios, obtained by comparing the (15)NH4(+) to total ammonium (via autoanalyzer) concentrations, are compared to the ratios of prepared standards. The OX/MIMS method requires only small sample volumes of water (ca. 12 mL) or sediment slurries and is rapid, convenient, accurate, and precise (R(2) = 0.9994, p < 0.0001) over a range of salinities and (15)N/(14)N ratios. It can provide data needed to quantify rates of ammonium regeneration, potential ammonium uptake, and dissimilatory nitrate reduction to ammonium (DNRA). Isotope ratio results agreed closely (R = 0.998, P = 0.001) with those determined independently by isotope ratio mass spectrometry for DNRA measurements or by ammonium isotope retention time shift liquid chromatography for water-column N-cycling experiments. Application of OX/MIMS should simplify experimental approaches and improve understanding of N-cycling rates and fate in a variety of freshwater and marine environments.

  12. Evaluation of core physics analysis methods for conversion of the INL advanced test reactor to low-enrichment fuel

    International Nuclear Information System (INIS)

    DeHart, M. D.; Chang, G. S.

    2012-01-01

    Computational neutronics studies to support the possible conversion of the ATR to LEU are underway. Simultaneously, INL is engaged in a physics methods upgrade project to put into place modern computational neutronics tools for future support of ATR fuel cycle and experiment analysis. A number of experimental measurements have been performed in the ATRC in support of the methods upgrade project, and are being used to validate the new core physics methods. The current computational neutronics work is focused on performance of scoping calculations for the ATR core loaded with a candidate LEU fuel design. This will serve as independent confirmation of analyses that have been performed previously, and will evaluate some of the new computational methods for analysis of a candidate LEU fuel for ATR. (authors)

  13. Clustering based gene expression feature selection method: A computational approach to enrich the classifier efficiency of differentially expressed genes

    KAUST Repository

    Abusamra, Heba; Bajic, Vladimir B.

    2016-01-01

    decrease the computational time and cost, but also improve the classification performance. Among different approaches of feature selection methods, however most of them suffer from several problems such as lack of robustness, validation issues etc. Here, we

  14. Clustering based gene expression feature selection method: A computational approach to enrich the classifier efficiency of differentially expressed genes

    KAUST Repository

    Abusamra, Heba

    2016-07-20

    The native nature of high dimension low sample size of gene expression data make the classification task more challenging. Therefore, feature (gene) selection become an apparent need. Selecting a meaningful and relevant genes for classifier not only decrease the computational time and cost, but also improve the classification performance. Among different approaches of feature selection methods, however most of them suffer from several problems such as lack of robustness, validation issues etc. Here, we present a new feature selection technique that takes advantage of clustering both samples and genes. Materials and methods We used leukemia gene expression dataset [1]. The effectiveness of the selected features were evaluated by four different classification methods; support vector machines, k-nearest neighbor, random forest, and linear discriminate analysis. The method evaluate the importance and relevance of each gene cluster by summing the expression level for each gene belongs to this cluster. The gene cluster consider important, if it satisfies conditions depend on thresholds and percentage otherwise eliminated. Results Initial analysis identified 7120 differentially expressed genes of leukemia (Fig. 15a), after applying our feature selection methodology we end up with specific 1117 genes discriminating two classes of leukemia (Fig. 15b). Further applying the same method with more stringent higher positive and lower negative threshold condition, number reduced to 58 genes have be tested to evaluate the effectiveness of the method (Fig. 15c). The results of the four classification methods are summarized in Table 11. Conclusions The feature selection method gave good results with minimum classification error. Our heat-map result shows distinct pattern of refines genes discriminating between two classes of leukemia.

  15. Medida de la dureza de sólidos mediante nanoindentación

    Directory of Open Access Journals (Sweden)

    Alkorta, J.

    2005-10-01

    Full Text Available Hardness is not readily measurable by means of instrumented indentation since the value of the contact area depends on the pile-up or sink-in occurring near the contact surface of the sample. The most widespread method to estimate it by means of the loading/unloading curve of indentation, Oliver and Pharr’s method, deviates, in the extreme cases, up to a 25% from the real values since it only takes into account the elastic deflection. In this work, a new correction based on Oliver and Pharr’s method is proposed that agrees with the numerical calculations. Plastic hardening behaviour of the sample must be known to accurately estimate the contact area.

    La medida de la dureza mediante indentación con registro de carga y desplazamiento no es evidente, dada la incertidumbre sobre el tamaño de huella debido al levantamiento (pile-up o hundimiento (sink-in plásticos de la superficie de la muestra alrededor del indentador. El método más utilizado para la medida de la dureza mediante la curva de carga/descarga de indentación, el de Oliver y Pharr, sólo tiene en cuenta hundimiento elástico, por lo que el error en la medida de la dureza y el módulo de Young puede llegar hasta un 25% en los casos más extremos. En el presente trabajo se discute una posible corrección al método de Oliver y Pharr para una obtención más ajustada del área de contacto de la huella. Esta corrección requiere de un conocimiento a priori o a posteriori del comportamiento plástico del material.

  16. Real time alpha value measurement with Feynman-α method utilizing time series data acquisition on low enriched uranium system

    International Nuclear Information System (INIS)

    Tonoike, Kotaro; Yamamoto, Toshihiro; Watanabe, Shoichi; Miyoshi, Yoshinori

    2003-01-01

    As a part of the development of a subcriticality monitoring system, a system which has a time series data acquisition function of detector signals and a real time evaluation function of alpha value with the Feynman-alpha method was established, with which the kinetic parameter (alpha value) was measured at the STACY heterogeneous core. The Hashimoto's difference filter was implemented in the system, which enables the measurement at a critical condition. The measurement result of the new system agreed with the pulsed neutron method. (author)

  17. Enriching the gene set analysis of genome-wide data by incorporating directionality of gene expression and combining statistical hypotheses and methods

    Science.gov (United States)

    Väremo, Leif; Nielsen, Jens; Nookaew, Intawat

    2013-01-01

    Gene set analysis (GSA) is used to elucidate genome-wide data, in particular transcriptome data. A multitude of methods have been proposed for this step of the analysis, and many of them have been compared and evaluated. Unfortunately, there is no consolidated opinion regarding what methods should be preferred, and the variety of available GSA software and implementations pose a difficulty for the end-user who wants to try out different methods. To address this, we have developed the R package Piano that collects a range of GSA methods into the same system, for the benefit of the end-user. Further on we refine the GSA workflow by using modifications of the gene-level statistics. This enables us to divide the resulting gene set P-values into three classes, describing different aspects of gene expression directionality at gene set level. We use our fully implemented workflow to investigate the impact of the individual components of GSA by using microarray and RNA-seq data. The results show that the evaluated methods are globally similar and the major separation correlates well with our defined directionality classes. As a consequence of this, we suggest to use a consensus scoring approach, based on multiple GSA runs. In combination with the directionality classes, this constitutes a more thorough basis for an enriched biological interpretation. PMID:23444143

  18. Various Solution Methods, Accompanied by Dynamic Investigation, for the Same Problem as a Means for Enriching the Mathematical Toolbox

    Science.gov (United States)

    Oxman, Victor; Stupel, Moshe

    2018-01-01

    A geometrical task is presented with multiple solutions using different methods, in order to show the connection between various branches of mathematics and to highlight the importance of providing the students with an extensive 'mathematical toolbox'. Investigation of the property that appears in the task was carried out using a computerized tool.

  19. Direct PCR - A rapid method for multiplexed detection of different serotypes of Salmonella in enriched pork meat samples

    DEFF Research Database (Denmark)

    Chin, Wai Hoe; Sun, Yi; Høgberg, Jonas

    2017-01-01

    , in this study, we developed a multiplex Direct PCR method for rapid detection of different Salmonella serotypes directly from pork meat samples without any DNA purification steps. An inhibitor-resistant Phusion Pfu DNA polymerase was used to overcome PCR inhibition. Four pairs of primers including a pair...

  20. An efficient and target-oriented sample enrichment method for preparative separation of minor alkaloids by pH-zone-refining counter-current chromatography.

    Science.gov (United States)

    Feng, Rui-Hong; Hou, Jin-Jun; Zhang, Yi-Bei; Pan, Hui-Qin; Yang, Wenzhi; Qi, Peng; Yao, Shuai; Cai, Lu-Ying; Yang, Min; Jiang, Bao-Hong; Liu, Xuan; Wu, Wan-Ying; Guo, De-An

    2015-08-28

    An efficient and target-oriented sample enrichment method was established to increase the content of the minor alkaloids in crude extract by using the corresponding two-phase solvent system applied in pH-zone-refining counter-current chromatography. The enrichment and separation of seven minor indole alkaloids from Uncaria rhynchophylla (Miq.) Miq. ex Havil(UR) were selected as an example to show the advantage of this method. An optimized two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (3:7:1:9, v/v) was used in this study, where triethylamine (TEA) as the retainer and hydrochloric acid (HCl) as the eluter were added at the equimolar of 10mM. Crude alkaloids of UR dissolved in the corresponding upper phase (containing 10mM TEA) were extracted twice with lower phase (containing 10mM TEA) and lower phase (containing 10mM HCl), respectively, the second lower phase extract was subjected to pH-zone-refining CCC separation after alkalization and desalination. Finally, from 10g of crude alkaloids, 4g of refined alkaloids was obtained and the total content of seven target indole alkaloids was increased from 4.64% to 15.78%. Seven indole alkaloids, including 54mg isocorynoxeine, 21mg corynoxeine, 46mg isorhynchophylline, 35mg rhynchophylline, 65mg hirsutine, 51mg hirsuteine and 27mg geissoschizine methylether were all simultaneously separated from 2.5g of refined alkaloids, with the purity of 86.4%, 97.5%, 90.3%, 92.1%, 98.5%, 92.3%, and 92.8%, respectively. The total content and purities of the seven minor indole alkaloids were tested by HPLC and their chemical structures were elucidated by ESI-HRMS and (1)H NMR. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Efficient enrichment of hepatic cancer stem-like cells from a primary rat HCC model via a density gradient centrifugation-centered method.

    Directory of Open Access Journals (Sweden)

    Wei-hui Liu

    Full Text Available BACKGROUND: Because few definitive markers are available for hepatic cancer stem cells (HCSCs, based on physical rather than immunochemical properties, we applied a novel method to enrich HCSCs. METHODOLOGY: After hepatic tumor cells (HTCs were first isolated from diethylinitrosamine-induced F344 rat HCC model using percoll discontinuous gradient centrifugation (PDGC and purified via differential trypsinization and differential attachment (DTDA, they were separated into four fractions using percoll continuous gradient centrifugation (PCGC and sequentially designated as fractions I-IV (FI-IV. Morphological characteristics, mRNA and protein levels of stem cell markers, proliferative abilities, induced differentiation, in vitro migratory capacities, in vitro chemo-resistant capacities, and in vivo malignant capacities were determined for the cells of each fraction. FINDINGS: As the density of cells increased, 22.18%, 11.62%, 4.73% and 61.47% of primary cultured HTCs were segregated in FI-FIV, respectively. The cells from FIII (density between 1.041 and 1.062 g/ml displayed a higher nuclear-cytoplasmic ratio and fewer organelles and expressed higher levels of stem cell markers (AFP, EpCAM and CD133 than cells from other fractions (P<0.01. Additionally, in vitro, the cells from FIII showed a greater capacity to self-renew, differentiate into mature HTCs, transit across membranes, close scratches, and carry resistance to chemotherapy than did cells from any other fraction; in vivo, injection of only 1×10(4 cells from FIII could generate tumors not only in subcutaneous tissue but also in the livers of nude mice. CONCLUSIONS: Through our novel method, HCSC-like cells were successfully enriched in FIII. This study will greatly contribute to two important areas of biological interest: CSC isolation and HCC therapy.

  2. Other enrichment related contracts

    International Nuclear Information System (INIS)

    Hall, J.C.

    1978-01-01

    In addition to long-term enrichment contracts, DOE has other types of contracts: (1) short-term, fixed-commitment enrichment contract; (2) emergency sales agreement for enriched uranium; (3) feed material lease agreement; (4) enriched uranium storage agreement; and (5) feed material usage agreement

  3. Quantification of Listeria monocytogenes in minimally processed leafy vegetables using a combined method based on enrichment and 16S rRNA real-time PCR.

    Science.gov (United States)

    Aparecida de Oliveira, Maria; Abeid Ribeiro, Eliana Guimarães; Morato Bergamini, Alzira Maria; Pereira De Martinis, Elaine Cristina

    2010-02-01

    Modern lifestyle markedly changed eating habits worldwide, with an increasing demand for ready-to-eat foods, such as minimally processed fruits and leafy greens. Packaging and storage conditions of those products may favor the growth of psychrotrophic bacteria, including the pathogen Listeria monocytogenes. In this work, minimally processed leafy vegetables samples (n = 162) from retail market from Ribeirão Preto, São Paulo, Brazil, were tested for the presence or absence of Listeria spp. by the immunoassay Listeria Rapid Test, Oxoid. Two L. monocytogenes positive and six artificially contaminated samples of minimally processed leafy vegetables were evaluated by the Most Probable Number (MPN) with detection by classical culture method and also culture method combined with real-time PCR (RTi-PCR) for 16S rRNA genes of L. monocytogenes. Positive MPN enrichment tubes were analyzed by RTi-PCR with primers specific for L. monocytogenes using the commercial preparation ABSOLUTE QPCR SYBR Green Mix (ABgene, UK). Real-time PCR assay presented good exclusivity and inclusivity results and no statistical significant difference was found in comparison with the conventional culture method (p < 0.05). Moreover, RTi-PCR was fast and easy to perform, with MPN results obtained in ca. 48 h for RTi-PCR in comparison to 7 days for conventional method.

  4. Fluxo de seiva pelo método do balanço de calor: base teórica, qualidade das medidas e aspectos práticos Sap flow by heat balance method: theoretical basis, data quality and practical aspects

    Directory of Open Access Journals (Sweden)

    Fábio Ricardo Marin

    2008-01-01

    Full Text Available O método do balanço de calor representa uma técnica importante para estudos sobre fisiologia vegetal, agrometeorologia e irrigação. Sua aplicação tem aumentado nos últimos anos em decorrência da maior facilidade de importação de material e equipamentos e do uso mais disseminado dos sistemas automáticos de aquisição de dados. Avanços importantes em diversas áreas de pesquisa têm sido alcançados com a possibilidade de se quantificar o consumo hídrico em plantas isoladas em diferentes escalas de tempo. Com base na teoria do método, esta revisão tem como objetivo apresentar e discutir os procedimentos de instalação, manutenção e de análise e processamento dos dados com vistas ao uso mais adequado da técnica e à obtenção de dados mais confiáveis e coerentes. Aspectos relacionados à influência das condições ambientais no funcionamento do sensor são abordados; são também discutidas diversas recomendações práticas observadas na literatura disponível. O grande número e a diversidade de procedimentos que envolvem a instalação dos sensores, sua manutenção em condições de campo e a análise dos dados conferem ao método grande aplicação na experimentação científica. Entretanto, o uso do método de balanço de calor em larga escala parece inadequado devido à dificuldade de correta operação, o que facilmente compromete a qualidade das medidas.The stem heat balance method has become an important technique in plant physiology, agrometeorology and irrigation science. Its application has increased in the last years due to the development of less expensive automatic data acquisition systems that are widespread. Important advances in several research areas have been reached by quantifying the water consumption by isolated plants in different temporal scales. Based on the theory of the method, this review aims to present and discuss the procedures of installation and maintenance, and data analysis and

  5. Trabajos en espacios confinados. Riesgos y medidas preventivas

    OpenAIRE

    Fernández, Sergio

    2012-01-01

    La ejecución de trabajos en espacios confinados resulta una necesidad en diversos sectores como por ejemplo en la industria química, redes de alcantarillado, construcción de barcos, en pozos,… con el fin desarrollar diversas tareas como limpiezas, inspecciones interiores, trabajos de pintura, reparaciones varias,… lo que implica la implantación de medidas especiales y rigurosas a planificar y llevar a cabo. Los riesgos que presentan la ejecución de estos trabajos en espacios confinados ...

  6. Plan de negocios de la empresa Random; pantalones a medida

    OpenAIRE

    Marsal Josa, Armando

    2017-01-01

    Este trabajo trata de la realización del Plan de Negocio de Random, idea de negocio que consiste en una startup que diseña y vende pantalones tipo vaquero y chino a medida, pudiendo el comprador elegir, de entre una serie de detalles de motivos navajos ofrecidos por la marca, la forma de personalizarlos. Tendrán un precio asequible, intermedio entre las grandes marcas (Zara...) y las marcas tradicionales (Levi’s...). Se pretende dar salida a un producto artesanal y de calidad, aprovechando un...

  7. A Medida socioeducativa de internação

    OpenAIRE

    Meggiato, Jaqueline da Rosa

    2011-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Sócio-Econômico, Programa de Pós-Graduação em Serviço Social, Florianópolis, 2011 O presente trabalho teve como preocupação central, investigar as diferentes correntes teóricas sobre o Direito da Criança e do Adolescente caracterizando-se, assim, como pesquisa de caráter teórico. A partir dessa investigação buscou-se entender os fundamentos que influenciam os magistrados em suas decisões pela medida socioeducativa de i...

  8. Confiabilidade da medida de espessuras musculares pela ultrassonografia Reliability of muscle thickness measurements using ultrasound

    Directory of Open Access Journals (Sweden)

    Paulo Sergio Chagas Gomes

    2010-02-01

    Full Text Available OBJETIVO: Determinar a confiabilidade das medidas de espessuras dos músculos flexores e extensores do cotovelo e joelho pela ultrassonografia (US, quantificando o erro típico associado a essas medidas (ETM. MÉTODOS: A confiabilidade (duas medidas interdias foi determinada em 15 voluntários aparentemente saudáveis (oito mulheres, 33,9 ± 11,4 anos, 76 ± 21kg, 170 ± 10cm. As imagens da musculatura flexora (FC e extensora do cotovelo (EC e flexora (FJ e extensora do joelho (EJ foram obtidas pela US bidimensional no modo B, utilizando transdutor de 7,5MHz. As espessuras do tecido muscular compreendidas entre as interfaces com o osso e com o tecido adiposo foram medidas em sítios anatômicos identificados e registrados para ser repetidos na segunda medida. RESULTADOS: A ANOVA não identificou diferenças significativas entre as medidas repetidas. Os coeficientes de correlação intraclasse foram FC = 0,970, EC = 0,971, FJ = 0,555 e EJ = 0,929 (P PURPOSE: To determine the reliability of muscle thickness measurements of elbow and knee flexors and extensors using ultrasound, and to quantify the typical error associated to the measurements (TEM. METHODS: The test-retest reliability was determined in 15 apparently healthy volunteers (8 women, 34 ± 11 years, 76 ± 21 kg, 170 ± 10 cm. The images of elbow flexors (EF and extensors (EE and knee flexors (KF and extensors (KE were obtained using a two dimensional mode B ultrasound instrument with a 7.5 MHz transducer. Muscle thickness between the adipose tissue and bone interfaces were measured at anatomical landmarks previously identified and recorded to assure the exact site for the retest. RESULTS: ANOVA did not identify any significant differences between the repeated measurements. Intraclass correlation coefficients (ICC of each pair of measure were EF = 0.970, EE = 0.971, KF = 0.555 e KE = 0.929 (P < 0.05 for all. The coefficients of variation were 3.9 %, 6.1 %, 6.6 % e 4.6 %, and TEM 1.3 mm, 1

  9. Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate

    Directory of Open Access Journals (Sweden)

    Ann Steele

    2012-01-01

    Full Text Available Background. Human cardiac-derived progenitor cells (hCPCs have shown promise in treating heart failure (HF in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA were obtained from HF patients (n=25; hAA-CHF. Minced tissues were suspended in complete (serum-containing DMEM. Cells were selected for their tissue migration and expression of stem cell factor receptor (hc-kit. Characterization of hc-kitpositive cells included immunohistochemical screening with a panel of monoclonal antibodies. Results. Cells, including phase-bright cells identified as hc-kitpositive, spontaneously emigrated from hAA-CHF in suspended explant cultures (SEC after Day 7. When cocultured with tissue, emigrated hc-kitpositive cells proliferated, first as loosely attached clones and later as multicellular clusters. At Day 21~5% of cells were hc-kitpositive. Between Days 14 and 28 hc-kitpositive cells exhibited mesodermal commitment (GATA-4positive and NKX2.5positive; then after Day 28 cardiac lineages (flk-1positive, smooth muscle actinpositive, troponin-Ipositive, and myosin light chainpositive. Conclusions. C-kitpositive hCPCs can be derived from atrial tissue of pediatric patients with end-stage HF. SEC is a novel culture method for derivation of migratory hc-kitpositive cells that favors clinical translation by reducing the need for exogenously added factors to expand hCPCs in vitro.

  10. PREFERENCE RANKING ORGANIZATION METHOD FOR ENRICHMENT EVALUATION(PROMETHEE SEBAGAI PENUNJANG KEPUTUSAN PEMILIHAN ANGGOTA BEM FMIPA UNLAM BANJARBARU

    Directory of Open Access Journals (Sweden)

    Megi Adhiyani

    2016-04-01

    Full Text Available The members election process of Student Executive Board of FMIPA UNLAM Banjarbaru is still done manually, so that the election of members require a long time and sometimes subjective. Thus, it needs a decision support system that could help ease the decision-making process using Promethee. Promethee is a method determining the sequence (priority to analyze the problems with the point is the simplicity, clarity, and stability (Arsita, 2013. From the result of the research and observation of the system has been made, can be known that Decision Support System for Member Election of Student Executive Board using Promethee get compliance 89.80 percent and the remaining 10.20 percent not in accordance with the decision of Student Executive Board of FMIPA UNLAM. Keywords : Decision Support System, Member Election of Student Executive Board, Promethee. Badan Eksekutif Mahasiswa (BEM FMIPA UNLAM Banjarbaru memiliki proses pemilihan anggota yang masih dilakukan secara manual sehingga pemilihan anggota BEM memerlukan waktu yang lama dan terkadang subjektif. Oleh karena itu, diperlukan sebuah sistem pendukung keputusan yang dapat mempermudah proses pengambilan keputusan menggunakan metode Promethee. Metode Promethee adalah suatu metode penentuan urutan (prioritas dalam analisa yang masalah pokoknya adalah kesederhanaan, kejelasan, dan kestabilan (2. Dari hasil penelitian dan pengamatan dari sistem yang telah dibuat, dapat diketahui bahwa Sistem Pendukung Keputusan Pemilihan Anggota BEM menggunakan metode Promethee ini mendapatkan nilai kesesuaian 89,80% dan sisanya 10,20% tidak sesuai dengan keputusan dari BEM FMIPA UNLAM. Kata kunci : Sistem Pendukung Keputusan, Pemilihan Anggota BEM, Metode Promethee.

  11. A new method to measure muscle protein synthesis in humans by endogenously introduced d9-leucine and using blood for precursor enrichment determination

    Science.gov (United States)

    Tran, Lee; Masters, Haley; Roust, Lori R; Katsanos, Christos S

    2015-01-01

    Enrichment from the easily accessible blood amino acid pool is commonly used as precursor enrichment to calculate rates of muscle protein fractional synthesis in relevant human studies in lieu of the less accessible muscle fluid amino acid pool. However, the accuracy of this approach depends largely on the extent to which there is low discrepancy in free amino acid enrichment between blood and muscle. Steady-state gradient (i.e., ratio) of amino acid enrichment between blood and muscle fluid in the basal state and in response to amino acid infusion were determined in five healthy subjects, and in association with two separate tracers: d9-leucine, introduced endogenously by the metabolism of d10-leucine (i.e., l-[2,3,3,4,5,5,5,6,6,6-2H10]leucine) infused in blood, and 13C6-phenylalanine introduced/infused in blood. The blood-to-muscle fluid amino acid enrichment ratio was lower (P enrichment introduced endogenously by intravenous infusion of d10-leucine provides a closer estimate of the muscle fluid amino acid enrichment, and its associated changes, than blood phenylalanine enrichment to calculate rates of muscle protein synthesis in humans. PMID:26243214

  12. Depleting high-abundant and enriching low-abundant proteins in human serum: An evaluation of sample preparation methods using magnetic nanoparticle, chemical depletion and immunoaffinity techniques.

    Science.gov (United States)

    de Jesus, Jemmyson Romário; da Silva Fernandes, Rafael; de Souza Pessôa, Gustavo; Raimundo, Ivo Milton; Arruda, Marco Aurélio Zezzi

    2017-08-01

    The efficiency of three different depletion methods to remove the most abundant proteins, enriching those human serum proteins with low abundance is checked to make more efficient the search and discovery of biomarkers. These methods utilize magnetic nanoparticles (MNPs), chemical reagents (sequential application of dithiothreitol and acetonitrile, DTT/ACN), and commercial apparatus based on immunoaffinity (ProteoMiner, PM). The comparison between methods shows significant removal of abundant protein, remaining in the supernatant at concentrations of 4.6±0.2, 3.6±0.1, and 3.3±0.2µgµL -1 (n=3) for MNPs, DTT/ACN and PM respectively, from a total protein content of 54µgµL -1 . Using GeLC-MS/MS analysis, MNPs depletion shows good efficiency in removing high molecular weight proteins (>80kDa). Due to the synergic effect between the reagents DTT and ACN, DTT/ACN-based depletion offers good performance in the depletion of thiol-rich proteins, such as albumin and transferrin (DTT action), as well as of high molecular weight proteins (ACN action). Furthermore, PM equalization confirms its efficiency in concentrating low-abundant proteins, decreasing the dynamic range of protein levels in human serum. Direct comparison between the treatments reveals 72 proteins identified when using MNP depletion (43 of them exclusively by this method), but only 20 proteins using DTT/ACN (seven exclusively by this method). Additionally, after PM treatment 30 proteins were identified, seven exclusively by this method. Thus, MNPs and DTT/ACN depletion can be simple, quick, cheap, and robust alternatives for immunochemistry-based protein depletion, providing a potential strategy in the search for disease biomarkers. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Camiones y dumpers: medidas de seguridad. (Tercera parte

    Directory of Open Access Journals (Sweden)

    Chinchilla, M.

    1963-04-01

    Full Text Available Safety measures to be adopted in connection with any type of work should be those that are specifically adequate for the kind of risks involved, for it is just as wrong to adopt measures that are too ellaborate as not to take any at all. Two kinds of safety measures can be considered: those applying to the personnel, and those that protect the equipment. The efficiency and trouble free completion of the work will depend on the nature of the safety measures that are taken. Accidents are easy to avoid in general if the right safety precautions are taken. Such accidents involve both moral and financial damage, and a consequent loss of efficiency.Las medidas de seguridad que deberán adoptarse en cualquier trabajo serán las precisas, puesto que puede ser tan perjudicial señalar demasiadas como no señalar ninguna. Pueden considerarse dos tipos de medidas de seguridad: las concernientes a la seguridad del personal y las referentes a la maquinaria empleada. De la determinación de unas y otras, así como de su exacta observancia, dependerá que el trabajo se realice de forma eficaz y sin contratiempos, fácilmente evitables, por otra parte, que acarrearían consecuencias de tipo moral y económico, con el consiguiente descenso del rendimiento obtenido.

  14. Derived enriched uranium market

    International Nuclear Information System (INIS)

    Rutkowski, E.

    1996-01-01

    The potential impact on the uranium market of highly enriched uranium from nuclear weapons dismantling in the Russian Federation and the USA is analyzed. Uranium supply, conversion, and enrichment factors are outlined for each country; inventories are also listed. The enrichment component and conversion components are expected to cause little disruption to uranium markets. The uranium component of Russian derived enriched uranium hexafluoride is unresolved; US legislation places constraints on its introduction into the US market

  15. Nuevo criterio de complejidad utilizando una medida de eficiencia

    OpenAIRE

    Munduate del Río, Arantza; Pérez Prados, Antonio; Cano Sevilla, Francisco J.

    1995-01-01

    Centrado en el campo de los árboles de decisión, este trabajo estudia la forma de selección de un árbol óptimo entre los posibles árbo1es obtenidos a partir del análisis de un conjunto de datos, utilizando para ello una cantidad criterio que combina linealmente dos medidas de la calidad de un árbol: el error de resustitución y la eficiencia. Analizando el efecto de un proceso de poda en la cantidad criterio se obtiene una sucesión finita de subárboles rnínimos óptimamente podados del árbol má...

  16. Refração atmosferica nas medidas Doppler

    OpenAIRE

    Oliveira, Leonardo Castro de

    1990-01-01

    Orientador: Jose Bittencourt de Andrade Dissertação (mestrado) - Universidade Federal do Parana. Setor de Tecnologia Resumo: Esta dissertação tem por objetivo realizar investigações referentes à refração atmosférica nas medidas Doppler. São considerados quatro modelos para correção troposférica, e o modelo de duas frequências para a correção da refração ionosférica . São também testados de diferentes fontes de dados meteorológicos. Todos os testes são feitos utilizando-se o programa GEO...

  17. A Simple Method for Measuring Carbon-13 Fatty Acid Enrichment in the Major Lipid Classes of Microalgae Using GC-MS

    Science.gov (United States)

    Elahee Doomun, Sheik Nadeem; Loke, Stella; O’Callaghan, Sean; Callahan, Damien L.

    2016-01-01

    A simple method for tracing carbon fixation and lipid synthesis in microalgae was developed using a combination of solid-phase extraction (SPE) and negative ion chemical ionisation gas chromatography mass spectrometry (NCI-GC-MS). NCI-GC-MS is an extremely sensitive technique that can produce an unfragmented molecular ion making this technique particularly useful for stable isotope enrichment studies. Derivatisation of fatty acids using pentafluorobenzyl bromide (PFBBr) allows the coupling of the high separation efficiency of GC and the measurement of unfragmented molecular ions for each of the fatty acids by single quadrupole MS. The key is that isotope spectra can be measured without interference from co-eluting fatty acids or other molecules. Pre-fractionation of lipid extracts by SPE allows the measurement of 13C isotope incorporation into the three main lipid classes (phospholipids, glycolipids, neutral lipids) in microalgae thus allowing the study of complex lipid biochemistry using relatively straightforward analytical technology. The high selectivity of GC is necessary as it allows the collection of mass spectra for individual fatty acids, including cis/trans isomers, of the PFB-derivatised fatty acids. The combination of solid-phase extraction and GC-MS enables the accurate determination of 13C incorporation into each lipid pool. Three solvent extraction protocols that are commonly used in lipidomics were also evaluated and are described here with regard to extraction efficiencies for lipid analysis in microalgae. PMID:27845718

  18. Uranium enrichment plans

    International Nuclear Information System (INIS)

    Thomas, D.C.; Gagne, R.W.

    1978-01-01

    The following topics are covered: the status of the Government's existing uranium enrichment services contracts, natural uranium requirements based on the latest contract information, uncertainty in predicting natural uranium requirements based on uranium enrichment contracts, and domestic and foreign demand assumed in enrichment planning

  19. Trace analysis of halogenated hydrocarbons in gaseous samples by on-line enrichment in an adsorption trap, on-column cold-trapping and capillary gas chromatography. I.Method and instrumentation

    NARCIS (Netherlands)

    Noij, T.H.M.; Fabian, P.; Borchers, R.; Janssen, F.; Cramers, C.A.M.G.; Rijks, J.A.

    1987-01-01

    A method is described for the determination of halocarbons in gaseous samples down to the ppt level (1:1012, v/v), consisting of successive on-line sub-ambient enrichment on an adsorbent, on-column cryofocusing, capillary gas chromatography and electron-capture detection. The quantitative aspects of

  20. Estudio de medidas y proyectos de movilidad ambiental en el entorno europeo

    OpenAIRE

    Moya González, Luis

    2011-01-01

    Índice Introducción Tablas resumen de medidas ambientales sobre movilidad Medidas específicas para ciudades históricas Mejora del transporte públicoGestión de automóvilesVehículos compartidosInnovación en vehículos y carburantesTransporte de mercancíasBicicletasPeatonesGestión: demanda, incentivos, etcOtras medidasMedidas coordinadas y planes integrales Experiencias europeasLa situación en EspañaProgramas y proyectos subvencionados Programas de la Unión EuropeaProyectos internacionales no vin...

  1. Uranium Enrichment, an overview

    International Nuclear Information System (INIS)

    Coates, J.H.

    1994-01-01

    This general presentation on uranium enrichment will be followed by lectures on more specific topics including descriptions of enrichment processes and assessments of the prevailing commercial and industrial situations. I shall therefore avoid as much as possible duplications with these other lectures, and rather dwell on: some theoretical aspects of enrichment in general, underlying the differences between statistical and selective processes, a review and comparison between enrichment processes, remarks of general order regarding applications, the proliferation potential of enrichment. It is noteworthy that enrichment: may occur twice in the LWR fuel cycle: first by enriching natural uranium, second by reenriching uranium recovered from reprocessing, must meet LWR requirements, and in particular higher assays required by high burn up fuel elements, bears on the structure of the entire front part of the fuel cycle, namely in the conversion/reconversion steps only involving UF 6 for the moment. (author). tabs., figs., 4 refs

  2. MEDIDAS DE NÚCLEO INFLACIONARIO PARA COSTA RICA

    Directory of Open Access Journals (Sweden)

    Gabriela Saborío Muñoz

    2003-07-01

    Full Text Available En este documento se elaboran y evalúan medidas alternativas de núcleo inflacionario para Costa Rica. La idea fundamental contempla al núcleo inflacionario como un indicador de la tendencia subyacente de la inflación capaz de capturar el componente del cambio total de precios común a todos los bienes y servicios, cuya persistencia se mantendría en el mediano y largo plazo y que excluye los cambios en los precios relativos de estos. La medida de núcleo inflacionario seleccionada se contrasta con el Índice de Núcleo Inflacionario (INI, indicador de inflación subyacente actualmente utilizado por el Banco Central de Costa Rica (BCCR.El Índice Subyacente de Inflación (ISI, definido como una medida de núcleo inflacionario que excluye un 30,7% del peso total del Índice de Precios al Consumidor (IPC, refleja más fielmente la tendencia subyacente de la inflación y logra capturar el movimiento más permanente del nivel general de precios, aislando las variaciones en precios relativos. Además, el ISI es fácil de calcular e interpretar lo cual ayuda a incrementar la transparencia y credibilidad de la política monetaria. También es un indicador oportuno, aumentando su valor para los que formulan la política monetaria. Por último, el ISI supera algunas de las limitaciones del INI, como son la falta de un criterio estadístico para definir el punto de corte de los bienes y servicios a excluir y el alto porcentaje del peso total del IPC eliminado. AbstractThis paper builds and evaluates several alternative measures of core inflation for Costa Rica. The chosen measure of core inflation is contrasted with the core inflation index (INI, which is the indicator of underlying inflation used today by the Central Bank of Costa Rica (BCCR. The main idea is that core inflation is a good indicator of the underlying inflation and catches the part of overall price change common to all the goods and services that is expected to persist in the

  3. A cellular automaton method to simulate the microstructure and evolution of low-enriched uranium (LEU) U–Mo/Al dispersion type fuel plates

    Energy Technology Data Exchange (ETDEWEB)

    Drera, Saleem S., E-mail: saleem.drera@gmail.com [Mechanical Engineering, Colorado School of Mines, Golden, CO 80401 (United States); Hofman, Gerard L. [Argonne National Laboratory, Chicago, IL 60439 (United States); Kee, Robert J. [Mechanical Engineering, Colorado School of Mines, Golden, CO 80401 (United States); King, Jeffrey C. [Metallurgical and Materials Engineering, Colorado School of Mines, Golden, CO 80401 (United States)

    2014-10-15

    Highlights: • This article presents a cellular automata (CA) algorithm to synthesize the growth of intermetallic interaction layers in U–Mo/Al dispersion fuel. • The method utilizes a 3D representation of the fuel, which is discretized into separate voxels that can change identy based on derived CA rules. • The CA model is compared to ILT measurements for RERTR experimental data. • The primary objective of the model is to synthesize three-dimensional microstructures that can be used in subsequent thermal and mechanical modeling. • The CA model can be used for predictive analysis. For example, it can be used to study the dependence of temperature on interaction layer growth. - Abstract: Low-enriched uranium (LEU) fuel plates for high power materials test reactors (MTR) are composed of nominally spherical uranium–molybdenum (U–Mo) particles within an aluminum matrix. Fresh U–Mo particles typically range between 10 and 100 μm in diameter, with particle volume fractions up to 50%. As the fuel ages, reaction–diffusion processes cause the formation and growth of interaction layers that surround the fuel particles. The growth rate depends upon the temperature and radiation environment. The cellular automaton algorithm described in this paper can synthesize realistic random fuel-particle structures and simulate the growth of the intermetallic interaction layers. Examples in the present paper pack approximately 1000 particles into three-dimensional rectangular fuel structures that are approximately 1 mm on each side. The computational approach is designed to yield synthetic microstructures consistent with images from actual fuel plates and is validated by comparison with empirical data on actual fuel plates.

  4. Present state of development of uranium enrichment

    International Nuclear Information System (INIS)

    1979-01-01

    The pilot plant for uranium enrichment started the operation on September 12, 1979. The pilot plant has been constructed by the Power Reactor and Nuclear Fuel Development Corp. in Ningyo Pass, Okayama Prefecture. 7000 centrifugal separators will be installed by mid 1981, and yearly production of 70 t SWU is expected. The Uranium Enrichment Committee of Japan Atomic Industrial Forum has made the proposal on the method of forwarding the development of uranium enrichment in Japan to Atomic Energy Commission and related government offices in December, 1978. This survey summarized the trends of uranium enrichment in Japan and foreign countries and the problems about nuclear non-proliferation, and provides with the reference materials. The demand and supply of uranium enrichment in the world, the present states and plans in USA, Europe, USSR and others, the demand and supply of uranium enrichment and the measures for securing it in Japan, the present state and future plan of uranium enrichment project in Japan, the international regulation of uranium enrichment, the recent policy of USA and INFCE, and the trend of the regulation of utilizing enriched uranium are described. Moreover, the concept of separation works in uranium enrichment and the various technologies of separation are explained. (Kako, I.)

  5. The changing face of enrichment

    International Nuclear Information System (INIS)

    Dunckel, E.

    1981-01-01

    The AIS techniques considered are atomic vapour laser isotope separation, molecular laser isotope separation and plasma separation. The future of the AIS technique and their advantages over the gas centrifuge method are discussed in terms of economics, power consideration, and possible enrichment contracts. (U.K.)

  6. Futebol libertário: compromisso social na medida

    Directory of Open Access Journals (Sweden)

    Fábio Silvestre Silva

    Full Text Available Baseados na Constituição de 1988 e no Estatuto da Criança e do Adolescente, de 1990, o Centro de Defesa dos Direitos da Criança e do Adolescente de Interlagos (CEDECA Interlagos traz um relato de experiência de uma metodologia alternativa de acompanhamento de adolescentes inseridos em medidas socioeducativas em meio aberto na região da Capela do Socorro e Parelheiros. Essa proposta utiliza o futebol como meio que possibilita ao adolescente refletir sobre sua realidade e suas práticas cotidianas, reconhecendo suas possibilidades de se desenvolver como sujeito de direito e de se tornar protagonista no que diz respeito às ações que busquem sua promoção social e a construção e o exercício da cidadania ativa. É possível observar que, com esse tipo de linguagem, mais próximo do desejo desses jovens, os mesmos atribuem um novo sentido ao acompanhamento a que foram submetidos. Na lógica socioeducativa, procuram superar obstáculos no sentido de buscar cada vez mais seu protagonismo, sua autoria, para reafirmar o que o ato infracional revelou, que é a busca por ser sujeito.

  7. Uranium enrichment: an overview

    International Nuclear Information System (INIS)

    Cazalet, J.

    1995-01-01

    This paper is a general presentation of uranium enrichment processes and assessments of the prevailing commercial and industrial situations. It gives first some theoretical aspects of enrichment in general and explains the differences between statistical and selective processes in particular. Then a review of the different processes is made with a comparison between them. Finally, some general remarks concerning applications are given and the risks of proliferation related to enrichment are mentioned. (J.S.). 4 refs., 5 figs., 8 tabs

  8. The enrichment secondary market

    International Nuclear Information System (INIS)

    Einbund, D.R.

    1986-01-01

    This paper will addresses two topics: the background to the present status of the enrichment secondary market and the future outlook of the secondary market in enrichment services, and the viability of the nuclear fuel brokerage industry. These two topics are inevitably connected, as most secondary market activity, not only in enrichment but also in natural uranium, has traditionally been conducted with the participation of brokers. Therefore, the author interrelates these topics

  9. Uranium enrichment plans

    International Nuclear Information System (INIS)

    Gagne, R.W.; Thomas, D.C.

    1977-01-01

    The status of existing uranium enrichment contracts in the US is reviewed and expected natural uranium requirements for existing domestic uranium enrichment contracts are evaluated. Uncertainty in natural uranium requirements associated with requirements-type and fixed-commitment type contracts is discussed along with implementation of variable tails assay

  10. Aplicação de medidas cefalométricas em 3D-TC: Application of cephalometric measurements in 3D-TC

    Directory of Open Access Journals (Sweden)

    Patrícia de Medeiros Loureiro Lopes

    2007-01-01

    Full Text Available OBJETIVO: o objetivo desta pesquisa foi avaliar a precisão e acurácia (validade de medidas cefalométricas lineares em imagens reconstruídas em terceira dimensão (3D, pela técnica de volume, a partir da tomografia computadorizada (TC multislice. METODOLOGIA: o material da pesquisa consistiu de 10 (dez crânios secos, previamente selecionados, sem distinção de etnia ou gênero, os quais foram submetidos à TC multislice 16 cortes com 0,5mm de espessura por 0,3mm de intervalo de reconstrução. Posteriormente, os dados obtidos foram enviados para uma estação de trabalho independente, contendo o programa Vitrea®. Os pontos cefalométricos (n=13 foram localizados e as respectivas medidas ósseas lineares (n=15 foram realizadas por 2 examinadores, previamente treinados, medindo cada um duas vezes, independentemente, em 3D. As medidas físicas foram obtidas por um terceiro examinador, utilizando um paquímetro digital. A análise dos dados foi realizada mediante um estudo comparativo entre as medidas inter e intra-examinadores, em 3D-TC, e entre estas e as medidas físicas obtidas diretamente nos crânios, utilizando ANOVA (análise de variância. RESULTADOS: não foram encontradas diferenças estatisticamente significantes entre os valores das medidas inter e intra-examinadores, nem entre as medidas físicas e em 3D, com p>0,6 para todas as medidas. O erro percentual foi de 2,05% para as medidas interexaminadores e de 2,11% para as medidas intra-examinadores. A média do erro percentual entre as medidas físicas e em 3D variou de 0,96% a 1,47%. CONCLUSÃO: todas as medidas cefalométricas lineares foram consideradas precisas e acuradas utilizando a técnica de volume em 3D por meio da TC multislice.AIM: To test the precision and accuracy of conventional linear cephalometric measurements in 3D reconstructed images using a multislice CT. METHODS: The study population consisted of 10 dry skulls, previously selected, without distinction of

  11. El prejuicio racial en Brasil: medidas comparativas O preconceito racial no Brasil: medidas comparativas Racial prejudice in Brazil: comparative measures

    Directory of Open Access Journals (Sweden)

    Ana Maria Lacerda Teixeira Pires

    2010-04-01

    Full Text Available El estudio trataba de medir las manifestaciones del prejuicio racial en una muestra de la población brasileña, utilizando las escalas de racismo moderno de McConahay, Hardee y Batts (1981 y la escala de racismo cordial de Turra y Venturi (1995 y Venturi (2003. Se aplicó los cuestionarios a un total de 101 estudiantes de diversas universidades en la región sur de Brasil. Los resultados muestran que las dos escalas difieren entre si respecto a captar las expresiones del prejuicio. Las personas declararon mayor racismo moderno que racismo cordial. También hemos estudiado las variables que influyen en el prejuicio racial declarado por los participantes. La variable género y orientación a la dominancia social son variables que predicen el racismo moderno en la muestra estudiada. Los participantes indicaron, en mayor medida, la manifestación del prejuicio racial de forma encubierta y lo hacen más abiertamente cuando hay la posibilidad del contacto personal y estrecho.O estudo avaliou as manifestações de preconceito racial em uma amostra da população brasileira, usando escalas de racismo moderno de McConahay, Hardee e Batts (1981, escala racismo cordial do Datafolha (1995 e Venturi, G. (2003. Os questionários foram aplicados a um total de 101 estudantes de várias universidades da região sul do Brasil. Os resultados mostram que as duas escalas diferem entre si sobre como capturar as expressões de preconceito. As pessoas relataram mais racismo moderno que racismo cordial. Estudamos também as variáveis que influenciam o preconceito racial declarado pelos participantes. As variáveis gênero e orientação à dominância social são preditores do racismo moderno na amostra estudada. Os participantes indicaram, em maior medida, a manifestação do preconceito racial dissimulado e o fazem mais abertamente quando existe a possibilidade do contato pessoal e estreito.This study measured the manifestations of racial prejudice in a sample of

  12. Resonancia magnética cardiaca: medidas lineales y volumétricas en adultos sanos

    OpenAIRE

    Heredia Sánchez, Fabian Mauricio; Cabezas, Andrés Mauricio

    2015-01-01

    Introducción: La gran mayoría de las medidas de normalidad utilizadas para la interpretación de resonancia cardiaca son extrapoladas de las medidas de ecocardiografía. Los limitados registros de medidas de normalidad se encuentran ajustados en poblaciones extranjeras, no hay registros en latinoamericanos. Objetivo: Determinar las dimensiones cardiacas utilizando resonancia magnética en una población de personas sin antecedente médicos con repercusión cardiaca para lograr una muestra de val...

  13. Developments in uranium enrichment

    International Nuclear Information System (INIS)

    Mohrhauer, H.

    1995-01-01

    The enrichment services market is still characterized by overcapacities. While consumption worldwide will rise by some 15% to 39,000 t SWU/a over the next ten years, capacities amount to nearly 50,000 t SWU/a. The price for enrichment services probably has reached its all time low. Prices below U.S. $ 100/kg SWU are not likely to cover costs even of the economically most advanced enrichment processes. Urenco has prepared for the difficult enrichment business in the years to come by streamlining and cost cutting measures. The company intends to hold and increase its share of more than 10% in the world market. The uranium enrichment plant of Gronau will be expanded further. Expansion beyond 1000 t is subject to another permit being granted under the Atomic Energy Act, an application for which was filed in December 1994. Centrifuge technology is the superior enrichment technology, i.e., there is still considerable potential for further development. Construction of enrichment plants employing the centrifuge technology in the United States and in France is being pursued in various phases, from feasibility studies to licensing procedures. Before these plants could be implemented, however, considerable problems of organization would have to be solved, and the market would have to change greatly, respectively. The laser process, at the present time, does not seem to be able to develop into a major industrial competitor. (orig.) [de

  14. TRIGA low enrichment fuel

    International Nuclear Information System (INIS)

    Gietzen, A.

    1993-01-01

    Sixty TRIGA reactors have been sold and the earliest of these are now passing twenty years of operation. All of these reactors use the uranium zirconium hydride fuel (UZrH) which provides certain unique advantages arising out of its large prompt negative temperature coefficient, very low fission product release, and high temperature capability. Eleven of these Sixty reactors are conversions from plate fuel to TRIGA fuel which were made as a result of these advantages. With only a few exceptions, TRIGA reactors have always used low-enriched uranium (LEU) fuel with an enrichment of 19.9%. The exceptions have either been converted from the standard low-enriched fuel to the 70% enriched FLIP fuel in order to achieve extended lifetime, or are higher powered reactors which were designed for long life using 93%-enriched uranium during the time when the use and export of highly enriched uranium (HEU) was not restricted. The advent of international policies focusing attention on nonproliferation and safeguards made the HEU fuels obsolete. General Atomic immediately undertook a development effort (nearly two years ago) in order to be in a position to comply with these policies for all future export sales and also to provide a low-enriched alternative to fully enriched plate-type fuels. This important work was subsequently partially supported by the U.S. Department of Energy. The laboratory and production tests have shown that higher uranium densities can be achieved to compensate for reducing the enrichment to 20%, and that the fuels maintain the characteristics of the very thoroughly proven standard TRIGA fuels. In May of 1978, General Atomic announced that these fuels were available for TRIGA reactors and for plate-type reactors with power levels up to 15 MW with General Atomic's standard commercial warranty

  15. TRIGA low enrichment fuel

    International Nuclear Information System (INIS)

    Gietzen, A.

    1993-01-01

    Sixty TRIGA reactors have been sold and the earliest of these are now passing twenty years of operation. All of these reactors use the uranium-zirconium hydride fuel (UZrH) which provides certain unique advantages arising out of its large prompt negative temperature coefficient, very low fission product release, and high temperature capability. Eleven of these Sixty reactors are conversions from plate fuel to TRIGA fuel which were made as a result of these advantages. With only a few exceptions, TRIGA reactors have always used low-enriched-uranium (LEU) fuel with an enrichment of 19.9%. The exceptions have either been converted from the standard low-enriched fuel to the 70% enriched FLIP fuel in order to achieve extended lifetime, or are higher powered reactors which were designed for long life using 93%-enriched uranium during the time when the use and export of highly enriched uranium (HEU) was not restricted. The advent of international policies focusing attention on nonproliferation and safeguards made the HEU fuels obsolete. General Atomic immediately undertook a development effort (nearly two years ago) in order to be in a position to comply with these policies for all future export sales and also to provide a low-enriched alternative to fully enriched plate-type fuels. This important work was subsequently partially supported by the U.S. Department of Energy. The laboratory and production tests have shown that higher uranium densities can be achieved to compensate for reducing the enrichment to 20%, and that the fuels maintain the characteristics of the very thoroughly proven standard TRIGA fuels. In May of 1978, General Atomic announced that these fuels were available for TRIGA reactors and for plate-type reactors with power levels up to 15 MW with GA's standard commercial warranty

  16. Seq2Logo: a method for construction and visualization of amino acid binding motifs and sequence profiles including sequence weighting, pseudo counts and two-sided representation of amino acid enrichment and depletion

    DEFF Research Database (Denmark)

    Thomsen, Martin Christen Frølund; Nielsen, Morten

    2012-01-01

    Seq2Logo is a web-based sequence logo generator. Sequence logos are a graphical representation of the information content stored in a multiple sequence alignment (MSA) and provide a compact and highly intuitive representation of the position-specific amino acid composition of binding motifs, active...... related to amino acid enrichment and depletion. Besides allowing input in the format of peptides and MSA, Seq2Logo accepts input as Blast sequence profiles, providing easy access for non-expert end-users to characterize and identify functionally conserved/variable amino acids in any given protein...... sites, etc. in biological sequences. Accurate generation of sequence logos is often compromised by sequence redundancy and low number of observations. Moreover, most methods available for sequence logo generation focus on displaying the position-specific enrichment of amino acids, discarding the equally...

  17. Enrichment technology. Dependable vendor of gas centrifuges

    International Nuclear Information System (INIS)

    Anon.

    2011-01-01

    Enrichment Technology is an innovative, high-tech company that develops, manufactures and installs gas centrifuges for enriching uranium. In addition, Enrichment Technology designs enrichment plants that use gas centrifuge technology. This technology offers the most efficient and cost-effective method for enriching uranium yet: high-performance, safe technology that dominates the market with a global share of 45 percent. A determining factor in Enrichment Technology's success is its mission: supplying its customers with safe, reliable technology. Production of the centrifuges requires versatile know-how and collaboration between different departments as well as interdisciplinary teams at the various sites. More than 2000 operators at 8 sites in 5 countries contribute their individual knowledge and personal skills in order to produce this exceptional technology. The head office is in Beaconsfield near London and the operational headquarters are in Almelo in the Netherlands. There are other sites in Germany (Juelich und Gronau), Great Britain (Capenhurst) as well as project sites in the USA and France. Capenhurst is where experienced engineers design new enrichment plants and organise their construction. Centrifuge components are manufactured in Almelo and Juelich, while the pipework needed to connect up the centrifuges is produced at the site in Gronau. In Juelich, highly qualified scientists in interdisciplinary teams are continuously researching ways of improving the current centrifuges. Communication between specialists in the fields of chemistry, physics and engineering forms the basis for the company's success and the key to extending this leading position in the global enrichment market. (orig.)

  18. Enriched-air fluidized bed gasification using bench and pilot scale reactors of dairy manure with sand bedding based on response surface methods

    International Nuclear Information System (INIS)

    Nam, Hyungseok; Maglinao, Amado L.; Capareda, Sergio C.; Rodriguez-Alejandro, David Aaron

    2016-01-01

    Enriched-air gasification was performed in fluidized bed reactors using the processed dairy manure which was mixed with sand bedding. The effects of temperature, modified equivalence ratio (ER_m), and oxygen concentration on the gas products were investigated based on the statistical models using a bench-scale reactor in order to obtain empirical correlations. Then, the empirical equations were applied to compare the produced gases from a pilot-scale fluidized bed gasifier. The empirical and actual H_2 and CH_4 compositions were within a 10% error, while the sum of produced CO and CO_2 gases showed similar composition within 3% error. The most influential factors for the syngas heating value were temperature followed by the oxygen concentration and ER (equivalence ratio). The composition of H_2 (2.1–11.5%) and CO (5.9–20.3%) rose with an increase in temperature and oxygen concentration. The variation of CO_2 (16.8–31.6%) was mainly affected by the degree of oxygen concentration in the gasifying agent. The ranges of the LHV (lower heating value), carbon conversion efficiency and cold gas efficiency were discussed. An economic review showed favorable indications for on-site dairy manure gasification process for electric power based on the depreciable payback period and the power production costs. - Highlights: • Sand mixed dairy manure obtained directly from a dairy farm was processed and used. • Response surface methodology was used to investigate the enriched-air gasification. • Syngas results from bench and pilot scale gasifiers were compared and reviewed. • A highest LVH of 8 MJ/Nm"3 was obtained from the enriched-air gasification. • The power production costs were determined to be $0.053/kWh

  19. City model enrichment

    Science.gov (United States)

    Smart, Philip D.; Quinn, Jonathan A.; Jones, Christopher B.

    The combination of mobile communication technology with location and orientation aware digital cameras has introduced increasing interest in the exploitation of 3D city models for applications such as augmented reality and automated image captioning. The effectiveness of such applications is, at present, severely limited by the often poor quality of semantic annotation of the 3D models. In this paper, we show how freely available sources of georeferenced Web 2.0 information can be used for automated enrichment of 3D city models. Point referenced names of prominent buildings and landmarks mined from Wikipedia articles and from the OpenStreetMaps digital map and Geonames gazetteer have been matched to the 2D ground plan geometry of a 3D city model. In order to address the ambiguities that arise in the associations between these sources and the city model, we present procedures to merge potentially related buildings and implement fuzzy matching between reference points and building polygons. An experimental evaluation demonstrates the effectiveness of the presented methods.

  20. Una medida de inflación subyacente exclusion-based para el Perú

    OpenAIRE

    Hauer, Erik Sebastián

    2015-01-01

    Hay poco consenso en la literatura sobre el diseño y evaluación de medidas de inflación subyacente. Estudios previos han mostrado que las medidas más técnicas de influencia limitada tienen mejor rendimiento empírico. Sin embargo, es deseado que las medidas de inflación subyacente sean lo más transparentes y replicables posibles, lo cual usualmente resulta en medidas más simples “exclusion-based”. A este respecto, se utiliza una nueva metodología de extracción de señal a la inflación para enco...

  1. Network-based functional enrichment

    Directory of Open Access Journals (Sweden)

    Poirel Christopher L

    2011-11-01

    Full Text Available Abstract Background Many methods have been developed to infer and reason about molecular interaction networks. These approaches often yield networks with hundreds or thousands of nodes and up to an order of magnitude more edges. It is often desirable to summarize the biological information in such networks. A very common approach is to use gene function enrichment analysis for this task. A major drawback of this method is that it ignores information about the edges in the network being analyzed, i.e., it treats the network simply as a set of genes. In this paper, we introduce a novel method for functional enrichment that explicitly takes network interactions into account. Results Our approach naturally generalizes Fisher’s exact test, a gene set-based technique. Given a function of interest, we compute the subgraph of the network induced by genes annotated to this function. We use the sequence of sizes of the connected components of this sub-network to estimate its connectivity. We estimate the statistical significance of the connectivity empirically by a permutation test. We present three applications of our method: i determine which functions are enriched in a given network, ii given a network and an interesting sub-network of genes within that network, determine which functions are enriched in the sub-network, and iii given two networks, determine the functions for which the connectivity improves when we merge the second network into the first. Through these applications, we show that our approach is a natural alternative to network clustering algorithms. Conclusions We presented a novel approach to functional enrichment that takes into account the pairwise relationships among genes annotated by a particular function. Each of the three applications discovers highly relevant functions. We used our methods to study biological data from three different organisms. Our results demonstrate the wide applicability of our methods. Our algorithms are

  2. MEDIDA SOCIOEDUCATIVA DE INTERNAÇÃO: ESTRATÉGIA PUNITIVA OU PROTETIVA?

    Directory of Open Access Journals (Sweden)

    Andrea Cristina Coelho Scisleski

    2015-12-01

    Full Text Available ResumoEste artigo problematiza como os jovens em conflito com a lei são administrados nas medidas socioeducativas de internação e busca a discussão das diferenças entre as formas de atender aqueles que recebem medidas de proteção e aqueles que recebem medidas socioeducativas. No que concerne à gestão, encontra-se uma separação na administração das medidas de proteção e das medidas socioeducativas em meio aberto, geridas pela Assistência Social, e as medidas socioeducativas de internação, geridas pela Segurança Pública. A pesquisa se desenvolveu pelo método cartográfico, que abrangeu entrevistas realizadas com profissionais do sistema socioeducativo, visitas às unidades de internação, leitura de processos jurídicos e oitivas de audiências na Vara da Infância e da Juventude. Conclui-se que a proteção integral preconizada pelo ECA não atinge todos, como é o caso dos adolescentes em conflito com a lei que estão internados, tendo seus direitos violados em prol da manutenção da segurança da população.

  3. Listeria monocytogenes Strains Underrepresented during Selective Enrichment with an ISO Method Might Dominate during Passage through Simulated Gastric Fluid and In Vitro Infection of Caco-2 Cells

    Science.gov (United States)

    Zilelidou, Evangelia; Karmiri, Christina-Vasiliki; Zoumpopoulou, Georgia; Mavrogonatou, Eleni; Kletsas, Dimitris; Tsakalidou, Effie; Papadimitriou, Konstantinos; Drosinos, Eleftherios

    2016-01-01

    ABSTRACT Various Listeria monocytogenes strains may contaminate a single food product, potentially resulting in simultaneous exposure of consumers to multiple strains. However, due to bias in strain recovery, L. monocytogenes strains isolated from foods by selective enrichment (SE) might not always represent those that can better survive the immune system of a patient. We investigated the effect of cocultivation in tryptic soy broth with 0.6% yeast extract (TSB-Y) at 10°C for 8 days on (i) the detection of L. monocytogenes strains during SE with the ISO 11290-1:1996/Amd 1:2004 protocol and (ii) the in vitro virulence of strains toward the Caco-2 human colon epithelial cancer cell line following exposure to simulated gastric fluid (SGF; pH 2.0)-HCl (37°C). We determined whether the strains which were favored by SE would be effective competitors under the conditions of challenges related to gastrointestinal passage of the pathogen. Interstrain competition of L. monocytogenes in TSB-Y determined the relative population of each strain at the beginning of SE. This in turn impacted the outcome of SE (i.e., favoring survival of competitors with better fitness) and the levels exposed subsequently to SGF. However, strong growth competitors could be outcompeted after SGF exposure and infection of Caco-2 cells by strains outgrown in TSB-Y and underdetected (or even missed) during enrichment. Our data demonstrate a preferential selection of certain L. monocytogenes strains during enrichments, often not reflecting a selective advantage of strains during infection. These findings highlight a noteworthy scenario associated with the difficulty of matching the source of infection (food) with the L. monocytogenes isolate appearing to be the causative agent during listeriosis outbreak investigations. IMPORTANCE This report is relevant to understanding the processes involved in selection and prevalence of certain L. monocytogenes strains in different environments (i.e., foods or

  4. The competitive enrichment market

    International Nuclear Information System (INIS)

    Parks, J.W.; Huffman, F.C.

    1984-01-01

    With the enactment of the ''Private Ownership of Special Nuclear Materials Act'' in 1964, the U.S. Government made provisions to enter into the uranium enrichment services business. Since nuclear power was in its infancy and the Government was promoting its growth as well as trying to help U.S. industry sell reactors overseas, the initial contracts (Requirements Contracts) for enrichment services placed most of the risks associated with the supplying of the services on the Government. Projections of nuclear power additions continued to grow and in 1972 the Atomic Energy Commission (AEC) stopped contracting under Requirements Contracts in order to study which mode of contracting best suited the commercial development of the industry. In mid-1973, the AEC introduced the Long-Term Fixed Commitment (LTFC) contract which shifted the risk to the customer. By mid-1974, AEC had contracts which completely used the enrichment capacity of its complex and refused to accept requests for additional contracts. This action further convinced European nations that they should continue to develop their own enrichment capacity and resulted in the EURODIF and URENCO projects. Before this time the U.S. supplied 100% of the world market for enriching services

  5. Enrichment: Dealing with overcapacity

    International Nuclear Information System (INIS)

    Peterson, C.H.

    1989-01-01

    Today's surplus of enrichment capacity will continue until at least the end of this century. This will challenge the ingenuity of the separative work unit (SWU) suppliers as they attempt to keep market share and remain profitable in a very competitive marketplace. The utilities will be faced with attractive choices, but making the best choice will require careful analysis and increased attention to market factors. Current demand projections will probably prove too high to the extent that more reactors are canceled or delayed. The DOE has the vast majority of the unused capacity, so it will feel the most immediate impact of this large surplus in productive capacity. The DOE has responded to these market challenges by planning another reorganization of its enriching operations. Without a major agreement among the governments affected by the current surplus in enrichment capacity, the future will see lower prices, more competitive terms, and the gradual substitution of centrifuge or laser enrichment for the gaseous diffusion plants. The competition that is forcing the gaseous diffusion prices down to marginal cost will provide the long-term price basis for the enrichment industry

  6. Melting curve analysis after T allele enrichment (MelcaTle as a highly sensitive and reliable method for detecting the JAK2V617F mutation.

    Directory of Open Access Journals (Sweden)

    Soji Morishita

    Full Text Available Detection of the JAK2V617F mutation is essential for diagnosing patients with classical myeloproliferative neoplasms (MPNs. However, detection of the low-frequency JAK2V617F mutation is a challenging task due to the necessity of discriminating between true-positive and false-positive results. Here, we have developed a highly sensitive and accurate assay for the detection of JAK2V617F and named it melting curve analysis after T allele enrichment (MelcaTle. MelcaTle comprises three steps: 1 two cycles of JAK2V617F allele enrichment by PCR amplification followed by BsaXI digestion, 2 selective amplification of the JAK2V617F allele in the presence of a bridged nucleic acid (BNA probe, and 3 a melting curve assay using a BODIPY-FL-labeled oligonucleotide. Using this assay, we successfully detected nearly a single copy of the JAK2V617F allele, without false-positive signals, using 10 ng of genomic DNA standard. Furthermore, MelcaTle showed no positive signals in 90 assays screening healthy individuals for JAK2V617F. When applying MelcaTle to 27 patients who were initially classified as JAK2V617F-positive on the basis of allele-specific PCR analysis and were thus suspected as having MPNs, we found that two of the patients were actually JAK2V617F-negative. A more careful clinical data analysis revealed that these two patients had developed transient erythrocytosis of unknown etiology but not polycythemia vera, a subtype of MPNs. These findings indicate that the newly developed MelcaTle assay should markedly improve the diagnosis of JAK2V617F-positive MPNs.

  7. GOMA: functional enrichment analysis tool based on GO modules

    Institute of Scientific and Technical Information of China (English)

    Qiang Huang; Ling-Yun Wu; Yong Wang; Xiang-Sun Zhang

    2013-01-01

    Analyzing the function of gene sets is a critical step in interpreting the results of high-throughput experiments in systems biology.A variety of enrichment analysis tools have been developed in recent years,but most output a long list of significantly enriched terms that are often redundant,making it difficult to extract the most meaningful functions.In this paper,we present GOMA,a novel enrichment analysis method based on the new concept of enriched functional Gene Ontology (GO) modules.With this method,we systematically revealed functional GO modules,i.e.,groups of functionally similar GO terms,via an optimization model and then ranked them by enrichment scores.Our new method simplifies enrichment analysis results by reducing redundancy,thereby preventing inconsistent enrichment results among functionally similar terms and providing more biologically meaningful results.

  8. The Low Chamber Pancreatic Cancer Cells Had Stem-Like Characteristics in Modified Transwell System: Is It a Novel Method to Identify and Enrich Cancer Stem-Like Cells?

    Directory of Open Access Journals (Sweden)

    Dongqing Wang

    2014-01-01

    Full Text Available Cancer stem cells (CSCs or cancer-initiating cells (CICs play an important role in tumor initiation, progression, metastasis, chemoresistance, and recurrence. It is important to construct an effective method to identify and isolate CSCs for biotherapy of cancer. During the past years, many researchers had paid more attention to it; however, this method was still on seeking. Therefore, compared to the former methods that were used to isolate the cancer stem cell, in the present study, we tried to use modified transwell system to isolate and enrich CSCs from human pancreatic cancer cell lines (Panc-1. Our results clearly showed that the lower chamber cells in modified transwell system were easily forming spheres; furthermore, these spheres expressed high levels of stem cell markers (CD133/CD44/CD24/Oct-4/ESA and exhibited chemoresistance, underwent epithelial-to-mesenchymal transition (EMT, and possessed the properties of self-renewal in vitro and tumorigenicity in vivo. Therefore, we speculated that modified transwell assay system, as a rapid and effective method, can be used to isolate and enrich CSCs.

  9. Laser and gas centrifuge enrichment

    Energy Technology Data Exchange (ETDEWEB)

    Heinonen, Olli [Senior Fellow, Belfer Center for Science and International Affairs, Harvard Kennedy School, Cambridge, Massachusetts (United States)

    2014-05-09

    Principles of uranium isotope enrichment using various laser and gas centrifuge techniques are briefly discussed. Examples on production of high enriched uranium are given. Concerns regarding the possibility of using low end technologies to produce weapons grade uranium are explained. Based on current assessments commercial enrichment services are able to cover the global needs of enriched uranium in the foreseeable future.

  10. Medidas automatizadas de psicrômetro de termopar aspirado versus não aspirado

    Directory of Open Access Journals (Sweden)

    Antonio Ribeiro da Cunha

    2014-06-01

    Full Text Available O termopar ou par termoelétrico é utilizado na obtenção da temperatura do ar com ótima precisão, sensibilidade e com grande vantagem sobre o termômetro de mercúrio ou álcool, pois geram sinais elétricos que podem ser armazenados em sistemas automatizados. No entanto, quando se trata da obtenção da umidade relativa a partir de termopar, o processo se torna mais cauteloso devido a outros fatores envolvidos. Neste estudo, avaliaram-se dois conjuntos psicrômetros de termopar, aspirado e não aspirado, em relação a um conjunto Vaisala, no período de 17 a 31/01/2008 (15 dias. A partir de medidas de temperaturas de bulbos seco e úmido foram obtidas medidas da umidade relativa, e com isso, foi possível compará-las com as medidas obtidas pelo conjunto Vaisala. Os dois tipos de psicrômetros apresentaram precisão e exatidão nas medidas de temperatura do ar, e apenas precisão nas medidas de umidade relativa do ar, sendo que o psicrômetro aspirado foi o mais preciso. Apesar do psicrômetro não aspirado ter apresentado menor precisão nas medidas, ele é mais robusto porque independe do micro-ventilador de 12 VDC, o que faz dele um sensor com menos riscos de medidas inadequadas. Ambos os psicrômetros, aspirado e não aspirado, não medem com exatidão a umidade relativa do ar no período noturno.

  11. Oxygen enrichment incineration

    International Nuclear Information System (INIS)

    Kim, Jeong Guk; Yang, Hee Chul; Park, Geun Il; Kim, Joon Hyung

    2000-10-01

    Oxygen enriched combustion technology has recently been used in waste incineration. To apply the oxygen enrichment on alpha-bearing waste incineration, which is being developed, a state-of-an-art review has been performed. The use of oxygen or oxygen-enriched air instead of air in incineration would result in increase of combustion efficiency and capacity, and reduction of off-gas product. Especially, the off-gas could be reduced below a quarter, which might reduce off-gas treatment facilities, and also increase an efficiency of off-gas treatment. However, the use of oxygen might also lead to local overheating and high nitrogen oxides (NOx) formation. To overcome these problems, an application of low NOx oxy-fuel burner and recycling of a part of off-gas to combustion chamber have been suggested

  12. Oxygen enrichment incineration

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jeong Guk; Yang, Hee Chul; Park, Geun Il; Kim, Joon Hyung

    2000-10-01

    Oxygen enriched combustion technology has recently been used in waste incineration. To apply the oxygen enrichment on alpha-bearing waste incineration, which is being developed, a state-of-an-art review has been performed. The use of oxygen or oxygen-enriched air instead of air in incineration would result in increase of combustion efficiency and capacity, and reduction of off-gas product. Especially, the off-gas could be reduced below a quarter, which might reduce off-gas treatment facilities, and also increase an efficiency of off-gas treatment. However, the use of oxygen might also lead to local overheating and high nitrogen oxides (NOx) formation. To overcome these problems, an application of low NOx oxy-fuel burner and recycling of a part of off-gas to combustion chamber have been suggested.

  13. Enrichment of Acinetobacter spp. from food samples.

    Science.gov (United States)

    Carvalheira, Ana; Ferreira, Vânia; Silva, Joana; Teixeira, Paula

    2016-05-01

    Relatively little is known about the role of foods in the chain of transmission of acinetobacters and the occurrence of different Acinetobacter spp. in foods. Currently, there is no standard procedure to recover acinetobacters from food in order to gain insight into the food-related ecology and epidemiology of acinetobacters. This study aimed to assess whether enrichment in Dijkshoorn enrichment medium followed by plating in CHROMagar™ Acinetobacter medium is a useful method for the isolation of Acinetobacter spp. from foods. Recovery of six Acinetobacter species from food spiked with these organisms was compared for two selective enrichment media (Baumann's enrichment and Dijkshoorn's enrichment). Significantly (p enrichment. Next, the Dijkshoorn's enrichment followed by direct plating on CHROMagar™ Acinetobacter was applied to detect Acinetobacter spp. in different foods. Fourteen different presumptive acinetobacters were recovered and assumed to represent nine different strains on the basis of REP-PCR typing. Eight of these strains were identified by rpoB gene analysis as belonging to the species Acinetobacter johnsonii, Acinetobacter calcoaceticus, Acinetobacter guillouiae and Acinetobacter gandensis. It was not possible to identify the species level of one strain which may suggests that it represents a distinct species. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Centrifuge enrichment program

    International Nuclear Information System (INIS)

    Astley, E.R.

    1976-01-01

    Exxon Nuclear has been active in privately funded research and development of centrifuge enrichment technology since 1972. In October of 1975, Exxon Nuclear submitted a proposal to design, construct, and operate a 3000-MT SWU/yr centrifuge enrichment plant, under the provisions of the proposed Nuclear Fuel Assurance Act of 1975. The U.S. Energy Research and Development Administration (ERDA) accepted the proposal as a basis for negotiation. It was proposed to build a 1000-MT SWU/yr demonstration increment to be operational in 1982; and after successful operation for about one year, expand the facilities into a 3000-MT SWU/yr plant. As part of the overall centrifuge enrichment plant, a dedicated centrifuge manufacturing plant would be constructed; sized to support the full 3000-MT SWU/yr plant. The selection of the centrifuge process by Exxon Nuclear was based on an extremely thorough evaluation of current and projected enrichment technology; results show that the technology is mature and the process will be cost effective. The substantial savings in energy (about 93%) from utilization of the centrifuge option rather than gaseous diffusion is a compelling argument. As part of this program, Exxon Nuclear has a large hardware R and D program, plus a prototype centrifuge manufacturing capability in Malta, New York. To provide a full-scale machine and limited cascade test capability, Exxon Nuclear is constructing a $4,000,000 Centrifuge Test Facility in Richland, Washington. This facility was to initiate operations in the Fall of 1976. Exxon Nuclear is convinced that the centrifuge enrichment process is the rational selection for emergence of a commercial enrichment industry

  15. US enrichment reduction studies

    International Nuclear Information System (INIS)

    1979-06-01

    A major national program, the Reduced Enrichment Research and Test Reactor (RERTR) Program, is currently under way in the U.S., centered at the Argonne National Laboratory (ANL), to reduce the potential of research and test reactor fuels for increasing the proliferation of nuclear explosive devices. The main objective of the program is to provide the technical means by which the uranium enrichment to be used in these reactors can be reduced to less than 20% without significant economic and performance penalties. The criteria, basis and goals of the program are consistent with the results of a number of case studies which have been performed as part of the program

  16. Advanced uranium enrichment processes

    International Nuclear Information System (INIS)

    Clerc, M.; Plurien, P.

    1986-01-01

    Three advanced Uranium enrichment processes are dealt with in the report: AVLIS (Atomic Vapour LASER Isotope Separation), MLIS (Molecular LASER Isotope Separation) and PSP (Plasma Separation Process). The description of the physical and technical features of the processes constitutes a major part of the report. If further presents comparisons with existing industrially used enrichment technologies, gives information on actual development programmes and budgets and ends with a chapter on perspectives and conclusions. An extensive bibliography of the relevant open literature is added to the different subjects discussed. The report was drawn up by the nuclear research Centre (CEA) Saclay on behalf of the Commission of the European Communities

  17. Uranium Conversion & Enrichment

    Energy Technology Data Exchange (ETDEWEB)

    Karpius, Peter Joseph [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-02-06

    The isotopes of uranium that are found in nature, and hence in ‘fresh’ Yellowcake’, are not in relative proportions that are suitable for power or weapons applications. The goal of conversion then is to transform the U3O8 yellowcake into UF6. Conversion and enrichment of uranium is usually required to obtain material with enough 235U to be usable as fuel in a reactor or weapon. The cost, size, and complexity of practical conversion and enrichment facilities aid in nonproliferation by design.

  18. Reactor Physics Methods and Preconceptual Core Design Analyses for Conversion of the Advanced Test Reactor to Low-Enriched Uranium Fuel Annual Report for Fiscal Year 2012

    Energy Technology Data Exchange (ETDEWEB)

    David W. Nigg; Sean R. Morrell

    2012-09-01

    Under the current long-term DOE policy and planning scenario, both the ATR and the ATRC will be reconfigured at an appropriate time within the next several years to operate with low-enriched uranium (LEU) fuel. This will be accomplished under the auspices of the Reduced Enrichment Research and Test Reactor (RERTR) Program, administered by the DOE National Nuclear Security Administration (NNSA). At a minimum, the internal design and composition of the fuel element plates and support structure will change, to accommodate the need for low enrichment in a manner that maintains total core excess reactivity at a suitable level for anticipated operational needs throughout each cycle while respecting all control and shutdown margin requirements and power distribution limits. The complete engineering design and optimization of LEU cores for the ATR and the ATRC will require significant multi-year efforts in the areas of fuel design, development and testing, as well as a complete re-analysis of the relevant reactor physics parameters for a core composed of LEU fuel, with possible control system modifications. Ultimately, revalidation of the computational physics parameters per applicable national and international standards against data from experimental measurements for prototypes of the new ATR and ATRC core designs will also be required for Safety Analysis Report (SAR) changes to support routine operations with LEU. This report is focused on reactor physics analyses conducted during Fiscal Year (FY) 2012 to support the initial development of several potential preconceptual fuel element designs that are suitable candidates for further study and refinement during FY-2013 and beyond. In a separate, but related, effort in the general area of computational support for ATR operations, the Idaho National Laboratory (INL) is conducting a focused multiyear effort to introduce modern high-fidelity computational reactor physics software and associated validation protocols to replace

  19. Comparative proteomic assessment of matrisome enrichment methodologies

    Science.gov (United States)

    Krasny, Lukas; Paul, Angela; Wai, Patty; Howard, Beatrice A.; Natrajan, Rachael C.; Huang, Paul H.

    2016-01-01

    The matrisome is a complex and heterogeneous collection of extracellular matrix (ECM) and ECM-associated proteins that play important roles in tissue development and homeostasis. While several strategies for matrisome enrichment have been developed, it is currently unknown how the performance of these different methodologies compares in the proteomic identification of matrisome components across multiple tissue types. In the present study, we perform a comparative proteomic assessment of two widely used decellularisation protocols and two extraction methods to characterise the matrisome in four murine organs (heart, mammary gland, lung and liver). We undertook a systematic evaluation of the performance of the individual methods on protein yield, matrisome enrichment capability and the ability to isolate core matrisome and matrisome-associated components. Our data find that sodium dodecyl sulphate (SDS) decellularisation leads to the highest matrisome enrichment efficiency, while the extraction protocol that comprises chemical and trypsin digestion of the ECM fraction consistently identifies the highest number of matrisomal proteins across all types of tissue examined. Matrisome enrichment had a clear benefit over non-enriched tissue for the comprehensive identification of matrisomal components in murine liver and heart. Strikingly, we find that all four matrisome enrichment methods led to significant losses in the soluble matrisome-associated proteins across all organs. Our findings highlight the multiple factors (including tissue type, matrisome class of interest and desired enrichment purity) that influence the choice of enrichment methodology, and we anticipate that these data will serve as a useful guide for the design of future proteomic studies of the matrisome. PMID:27589945

  20. Análise da confiabilidade do método Figura Oito e da volumetria para mensuração do edema de tornozelo Análisis de la confiabilidad del método Figura Ocho y de la volumetria para medida del edema de tobillo Analysis of the Figure-of-Eight method and volumetry reliability for ankle edema measurement

    Directory of Open Access Journals (Sweden)

    Filipe Abdalla dos Reis

    2004-12-01

    Full Text Available A presença de lesões no tornozelo torna necessária a mensuração do edema como fator diagnóstico e evolutivo. O presente trabalho teve como objetivo analisar a confiabilidade do método Figura Oito e da volumetria para a mensuração do volume do tornozelo, intrateste e interteste. Vinte voluntários, sexo masculino, assintomáticos, idade entre 15 e 30 anos (X= 21,8, foram avaliados aleatoriamente por três examinadores; cada um realizou três mensurações utilizando o método Figura Oito e três, a volumetria. A ordem dos examinadores e da técnica de mensuração foi aleatória. Observou-se excelente confiabilidade nas duas técnicas (ICC = 0,99. O coeficiente de correlação momento-produto de Pearson demonstrou similaridade entre os examinadores; o primeiro apresentou r = 0,91, o segundo, r = 0,95 e o terceiro, r = 0,96. Concluiu-se que os dois métodos são confiáveis, apesar de a maioria dos autores recomendar o método Figura Oito pela sua praticidade, rapidez e baixo custo.La presencia de lesiones del tobillo hace necesario el medir la magnitud del edema como factor diagnóstico y evolutivo. El presente trabajo tiene como objetivo analisar la confiabilidad del metodo Figura Ocho y de la volumetría para la mensura del volumen del tobillo intratest e interteste. Veinte voluntarios, de sexo masculino, asintomáticos, de edades entre 15 a 30 años (X= 21.8, fueron avaluados aleatoriamente por tres examinadores, donde cada uno realizó tres medidas utilizando el método Figura Ocho y tres utilizando la volumetria. La orden de los examinadores y de la técnica de mensura fué aleatoria. Se ha observado una excelente confiabilidad en las dos técnicas (ICC = 0.99. El coeficiente de correlación momento-produto de Pearson demonstró similaridad entre los examinadores, siendo que el primero presentó r = 0.91, el segundo r = 0.95 y el tercero r = 0.96. Se concluye que los dos métodos son confiables, a pesar de la mayoría de los autores

  1. Online trapping and enrichment ultra performance liquid chromatography–tandem mass spectrometry method for sensitive measurement of “arginine-asymmetric dimethylarginine cycle” biomarkers in human exhaled breath condensate

    International Nuclear Information System (INIS)

    Di Gangi, Iole Maria; Pirillo, Paola; Carraro, Silvia; Gucciardi, Antonina; Naturale, Mauro; Baraldi, Eugenio; Giordano, Giuseppe

    2012-01-01

    Highlights: ► Simultaneous quantification of “arginine-ADMA cycle” metabolites developed in EBC. ► EBC is a non-invasive matrix highly useful in patients with respiratory diseases. ► Method, fast, precise and accurate, is suitable in the pediatric clinical studies. ► Sensitivity is increased using on-line trapping and enrichment-UPLC–MS/MS method. ► EBC measurements in asthmatic adolescents confirm that ADMA is increased in asthma. - Abstract: Background: Exhaled breath condensate (EBC) is a biofluid collected non invasively that, enabling the measurement of several biomarkers, has proven useful in the study of airway inflammatory diseases, including asthma, COPD and cystic fibrosis. To the best of our knowledge, there is no previous report of any analytical method to detect ADMA in EBC. Objectives: Aim of this work was to develop an online sample trapping and enrichment system, coupled with an UPLC–MS/MS method, for simultaneous quantification of seven metabolites related to “Arginine-ADMA cycle”, using the isotopic dilution. Methods: Butylated EBC samples were trapped in an online cartridge, washed before and after each injection with cleanup solution to remove matrix components and switched inline into the high pressure analytical column. Multiple reaction monitoring in positive mode was used for analyte quantification by tandem mass spectrometry. Results: Validation studies were performed in EBC to examine accuracy, precision and robustness of the method. For each compound, the calibration curves showed a coefficient of correlation (r 2 ) greater than 0.992. Accuracy (%Bias) was −1 ), measured in EBC samples of asthmatic adolescents are significantly increased (p < 0.0001) than in normal controls (0.0040 ± 0.0021 vs. 0.0012 ± 0.0005 and 0.0020 ± 0.0015 vs. 0.0002 ± 0.0001, respectively), as well the ADMA/Tyr (0.34 ± 0.09 vs. 0.12 ± 0.02, p < 0.0001) and the SDMA/Tyr ratio (0.10 ± 0.04 vs. 0.015 ± 0.004, p < 0.0001). Conclusions

  2. An Enriching Community.

    Science.gov (United States)

    Holland, Nancy A.; Burroughs, Jean

    2001-01-01

    Successful school-community partnerships in Volusia (Florida) Public Schools are the results of marketing creatively, meeting community members' needs, and bringing the right people together. The 3-year old program now offers students of all ages an expanding list of enrichment classes on many subjects for a nominal fee. (MLH)

  3. Requirements for enrichment tools

    NARCIS (Netherlands)

    Boer, A.; Winkels, R.; Trompper, M.

    2016-01-01

    This report gives a high level overview of requirements for Enrichment tools in the Openlaws.eu project. Openlaws.eu aims to initiate a platform and develop a vision for Big Open Legal Data (BOLD): an open framework for legislation, case law, and legal literature from across Europe.

  4. Enriching the Catalog

    Science.gov (United States)

    Tennant, Roy

    2004-01-01

    After decades of costly and time-consuming effort, nearly all libraries have completed the retrospective conversion of their card catalogs to electronic form. However, bibliographic systems still are really not much more than card catalogs on wheels. Enriched content that Amazon.com takes for granted--such as digitized tables of contents, cover…

  5. Process for recovering water enriched with deuterium

    International Nuclear Information System (INIS)

    Mandel, H.

    1975-01-01

    By the process proposed herewith, enrichment of deuterium in water by cooling water recirculation through series-connection of several cooling ciruits in the form of columns is obtained. With this method, conventional, open-type cooling towers without special installations can be applied, which is an important advantage as compared with a formerly proposed single-stage process with specially designed, complicated cooling towers. Series-connection of the cooling towers is carried out in such a way that the circulating water of a certain cooling circuit, which has a corresponding output value of deuterium enrichment, is conveyed to a succeeding circuit where further enrichment is achieved. The water enriched with deuterium is removed from the last cooling circuit of the series while an amount of fresch water equivalent to the water removed or evaporated altogether is fed to the first circuit of the series. (RB) [de

  6. The role of macrophytes in habitat structuring in aquatic ecosystems: methods of measurement, causes and consequences on animal assemblages' composition and biodiversity O papel das macrófitas na estruturação de habitat em ambientes aquáticos: métodos de medida, causas e consequências para a composição das assembléias animais e biodiversidade

    Directory of Open Access Journals (Sweden)

    Sidinei Magela Thomaz

    2010-06-01

    Full Text Available Aquatic macrophytes play an important role in structuring communities in aquatic environments. These plants provide physical structure, increase habitat complexity and heterogeneity and affect various organisms like invertebrates, fishes and waterbirds. The complexity provided by macrophytes has been exhaustively studied in aquatic environments. However, macrophyte complexity has rarely been measured in a standardized fashion, making comparisons among different studies and the establishment of general conclusions difficult. To address this issue, this review is focused on questions related to the habitat structural complexity provided by these plants, exploring: i how complexity has been viewed by ecologists, with an emphasis on macrophyte studies; ii the pros and cons of several methods used to quantify plant complexity; iii the consequences of habitat structuring by macrophytes on invertebrates and fish and possible causes, mediated by habitat complexity, that lead to changes in these animal assemblages; iv potential impacts of non-native macrophyte species on habitat complexity and v the importance of complexity provided by macrophytes to management strategies for maintaining aquatic biodiversity. We examined literature produced in both temperate and tropical regions, but prioritized the latter. We found a great variety of habitat complexity measurements that are applied to aquatic macrophytes to understand their influence on attached animal assemblages. A lack of standardization (considering the wide range of techniques and scales of resolution used limits comparisons between different studies exploring this subject, in which biological samples and physical substrates were used to explore these relationships. Macrophytes affect animal assemblages and promote biodiversity through a chain of mechanisms, related to habitat complexity, that involve the availability of shelter and feeding sites. Invasive macrophyte species may modify habitat

  7. Availability of enrichment services

    International Nuclear Information System (INIS)

    Svenke, E.

    1977-01-01

    The report summarizes major uncertainties which are likely to influence future demands for uranium isotopic enrichment. Since for the next decade the development of nuclear power will be largely concerned with the increment in demand the timely need for enrichment capacity will be particularly sensitive to assumptions about growth rates. Existing worldwide capacity together with capacities under construction will be sufficient well into the 1980's. However, long decision and construction leadtime, uncertainty as to future demand as well as other factors, specifically high capital need, all of which entail financial risks, create hindrances to a timely development of increment. The adequacy of current technology is well demonstrated in plant operation and new technology is under way. Technology is, however, not freely available on a purely commercial basis. Commercial willingness, which anticipates a limited degree of financial risk, is requesting both long term back-up from the utilities that would parallel their firm decisions on the acquisition of nuclear power units, and a protective government umbrella. This situation depends on the symbiotic relationship that exists between the nuclear power generating organizations, the enrichment undertakings and the governments involved. The report accordingly stresses the need for a more cooperative approach and this, moreover, at the multinational level. There is otherwise a risk that proper resources and financing means will not be allocated to the enrichment sector. Export limitations that request the highest degree of industrial processing of nuclear fuel, i.e. the compulsory enrichment of natural uranium, do not serve the interests of overall industrial efficiency

  8. Promotion of uranium enrichment business

    International Nuclear Information System (INIS)

    Kurushima, Morihiro

    1981-01-01

    The Committee on Nuclear Power has studied on the basic nuclear power policy, establishing its five subcommittees, entrusted by the Ministry of Nternational Trade and Industry. The results of examination by the subcommittee on uranium enrichment business are given along with a report in this connection by the Committee. In order to establish the nuclear fuel cycle, the aspect of uranium enrichment is essential. The uranium enrichment by centrifugal process has proceeded steadily in Power Reactor and Nuclear Fuel Development Corporation. The following matters are described: the need for domestic uranium enrichment, the outlook for overseas enrichment services and the schedule for establishing domestic enrichment business, the current state of technology development, the position of the prototype enrichment plant, the course to be taken to establish enrichment business the main organization operating the prototype and commercial plants, the system of supplying centrifuges, the domestic conversion of natural uranium the subsidies for uranium enrichment business. (J.P.N.)

  9. United States uranium enrichment policies

    International Nuclear Information System (INIS)

    Roberts, R.W.

    1977-01-01

    ERDA's uranium enrichment program policies governing the manner in which ERDA's enrichment complex is being operated and expanded to meet customer requirements for separative work, research and development activities directed at providing technology alternatives for future enrichment capacity, and establishing the framework for additional domestic uranium enrichment capacity to meet the domestic and foreign nuclear industry's growing demand for enrichment services are considered. The ERDA enrichment complex consists of three gaseous diffusion plants located in Oak Ridge, Tennessee; Paducah, Kentucky; and Portsmouth, Ohio. Today, these plants provide uranium enrichment services for commercial nuclear power generation. These enrichment services are provided under contracts between the Government and the utility customers. ERDA's program involves a major pilot plant cascade, and pursues an advanced isotope separation technique for the late 1980's. That the United States must develop additional domestic uranium enrichment capacity is discussed

  10. Producing deuterium-enriched products

    International Nuclear Information System (INIS)

    1980-01-01

    A method of producing an enriched deuterium product from a gaseous feed stream of mixed hydrogen and deuterium, comprises: (a) combining the feed stream with gaseous bromine to form a mixture of the feed stream and bromine and exposing the mixture to an electrical discharge effective to form deuterium bromide and hydrogen bromide with a ratio of D/H greater than the ratio of D/H in the feed stream; and (b) separating at least a portion of the hydrogen bromide and deuterium bromide from the mixture. (author)

  11. desarrollo y validación de un instrumento de medida

    Directory of Open Access Journals (Sweden)

    Arménio Rego

    2005-01-01

    Full Text Available El artículo describe cómo un instrumento de medida de la Inteligencia Emocional fue construido y validado. Cuatro etapas fueron seguidas: 1 Fueron escritos 96 ítems con base en la literatura y en trabajo propio de los autores; 2 Fue elaborado un cuestionario y aplicado a una muestra de 339 individuos; 3 Se procedió a un análisis factorial de las componentes principales; 4 Se relacionaron los factores emergentes con una medida de satisfacción con la vida. Los resultados sugirieron una solución factorial de seis componentes. Las consistencias internas de las escalas son invariablemente superiores a 0.70. Las seis dimensiones explican el 29% de la varianza de la medida de satisfacción con la vida

  12. Application of the 4 {sup {pi}{sup {gamma}}} Method to the Absolute Standardization of Radioactive Sources of Positron Emitters; Aplicacion del Metodo 4 {sup {pi}{sup {gamma}}} a la Medida Absoluta de la Actividad de Fuentes Radiactivas Emisoras de Positrones

    Energy Technology Data Exchange (ETDEWEB)

    Peyres Medina, V.; Garcia-Torano Martinez, E.; Roteta Ibarra, M.

    2006-07-01

    We discuss the application of the method known as 4 {sup {pi}{sup {gamma}}} counting to the standardization of positron emitters. Monte Carlo simulations are used to calculate the detection efficiency of positrons emitted by the nuclides 22Na and 18F. Two experimental setups are used, both based on a NaI(Tl) well detector. The results of the standardizations are in good agreement with those obtained by other methods. It is shown that the 4 {sup {pi}{sup {gamma}}} method can be successfully used for the absolute standardization of sources of positron emitters. (Author) 23 refs.

  13. New method of measurement IN VIVO Combination internal whole body with high-resolution germanium detectors. Rapid response in cross-contamination v; Nuevo metodo de medida in vivo de la combinacion interna en cuerpo entero con detectores de germanio de alta resolucion. respuesta rapida en contaminaciones cruzadas

    Energy Technology Data Exchange (ETDEWEB)

    Navarro Amaro, J. F.; Perez Lopez, B.; Lopez Ponte, M. A.; Navarro Bravo, T.

    2013-07-01

    This test method is a substantial improvement on the current capacities of the Personal Internal Dosimetry Service CIEMAT, as it will in a very short time make accurate measurements in vivo, excellent resolution and low background radiation environment in geometry whole body. The method will complement the existing laboratory [3] and may be used in emergencies or radiological accident in which the complexity of measuring spectra (cross contamination, multipicos interference) require a higher spectral resolution. (Author)

  14. Consecuencias de las medidas no arancelarias impuestas a Colombia por Ecuador y Argentina

    OpenAIRE

    Perez Giraldo, Johanna Sembelly; Vargas, William

    2016-01-01

    Con la apertura económica y comercial ha aumentado la negociación de acuerdos comerciales por medio de los cuales los países han logrado tener acceso preferencial libre de aranceles aduaneros. Esta dinámica ha incrementado el uso de medidas no arancelaria como instrumentos para controlar y restringir el acceso a los mercados de los países. Por lo expuesto, esta investigación presenta y analiza las medidas no arancelarias que fueron aplicadas a Colombia por Ecuador y Argentina desde el año 201...

  15. Ilustração de incertezas em medidas utilizando experimentos de queda livre

    OpenAIRE

    Coluci,Vitor R.; Paulino,Guilherme; Souza,Diego C. de; Vasconcelos,Elba P.R.

    2013-01-01

    O conhecimento da incerteza de uma medida é crucial para obter conclusões a partir de resultados experimentais. Erros aleatórios, comuns em experimentos, produzem uma distribuição dos valores medidos que segue o formato gaussiano. Neste trabalho desenvolvemos uma montagem baseada no fenômeno de queda livre para auxiliar na ilustração do conceito de incerteza em medidas. Os tempos de queda de um objeto foram obtidos automaticamente pela placa Arduino e exibidos na forma de um histograma na tel...

  16. Aplicaciones de los modelos multinivel al análisis de medidas repetidas en estudios longitudinales

    OpenAIRE

    María Victoria Zunzunegui; María Jesús García de Yébenes; Mathieu Forster; María Dolores Aguilar Conesa; Angel Rodríguez Laso; Ángel Otero

    2004-01-01

    Este trabajo es una introducción al análisis de medidas repetidas en estudios longitudinales. Se utiliza un marco analítico con dos etapas, ajustando modelos jerárquicos lineales con dos niveles. El primer nivel corresponde a la ocasión (tiempo) de medida y el segundo al individuo. Estos modelos estadísticos proceden de las ciencias sociales, en las que se han utilizado durante más de 25 años para analizar datos en organizaciones con múltiples niveles. Su aplicación permi...

  17. LAS MEDIDAS PREVENTIVAS AMBIENTALES, UNA APROXIMACIÓN DESDE EL DERECHO ADMINISTRATIVO.

    Directory of Open Access Journals (Sweden)

    Iván Andrés Páez Páez

    2013-05-01

    Full Text Available Las medidas preventivas previstas en el régimen sancionatorio ambiental sonuna especie de las medidas cautelares consagradas en el ordenamiento jurídicocolombiano, aunque con algunas particularidades propias de su ámbito deaplicación, que es el derecho ambiental. El artículo realiza una aproximaciónrespecto al contenido, alcance, antecedentes y justificación de las medidaspreventivas ambientales desde la óptica del derecho administrativo y comomanifestación de una de las funciones del ejecutivo, cual es el mantenimientodel orden público.

  18. Nodo sensor para la medida ambiental de CO2, temperatura y humedad

    OpenAIRE

    Jara Burgos, Marcos

    2007-01-01

    En este documento se describe el diseño y la implementación de un nodo sen-sor para la medida ambiental del nivel de CO2, la temperatura y la humedad relativa. El sistema se basa en un interfaz directo sensor a microcontrolador para las medidas de temperatura y humedad relativa, mientras que para el sensor de CO2 se basa en una conversión analógica digital. El sistema utiliza un sensor resistivo para medir la temperatura, un sensor capacitivo para medir la humedad relativa y un sensor...

  19. Medidas preventivas, correctoras y compensatorias del impacto ecológico de carreteras

    OpenAIRE

    Aizpurúa Giráldez, Nerea

    2011-01-01

    Las medidas preventivas, correctoras y compensatorias empleadas en los proyectos de carretera son uno de los elementos clave para conseguir la integración ambiental de estas infraestructuras. De ellas dependerá que los posibles impactos ambientales generados durante su construcción y explotación no supongan un detrimento inadmisible de la calidad ambiental. La tipología de medidas que se vienen aplicando desde hace más de veinte años en durante el proceso de Evaluación de Impacto Ambiental (E...

  20. Future of uranium enrichment

    International Nuclear Information System (INIS)

    Hosmer, C.

    1981-01-01

    The increasing amount of separative work being done in government facilities to produce low-enriched uranium fuel for nuclear utilities again raises the question: should this business-type, industrial function be burned over the private industry. The idea is being looked at by the Reagan administration, but faces problems of national security as well as from the unique nature of the business. This article suggests that a joint government-private venture combining enriching, reprocessing, and waste disposal could be the answer. Further, a separate entity using advanced laser technology to deplete existing uranium tails and lease them for fertile blankets in breeder reactors might earn substantial revenues to help reduce the national debt

  1. South Australia, uranium enrichment

    International Nuclear Information System (INIS)

    1976-02-01

    The Report sets out the salient data relating to the establishment of a uranium processing centre at Redcliff in South Australia. It is conceived as a major development project for the Commonwealth, the South Australian Government and Australian Industry comprising the refining and enrichment of uranium produced from Australian mines. Using the data currently available in respect of markets, demand, technology and possible financial return from overseas sales, the project could be initiated immediately with hexafluoride production, followed rapidly in stages by enrichment production using the centrifuge process. A conceptual development plan is presented, involving a growth pattern that would be closely synchronised with the mining and production of yellowcake. The proposed development is presented in the form of an eight-and-half-year programme. Costs in this Report are based on 1975 values, unless otherwise stated. (Author)

  2. Equipo comparativo para la medida de conductividad térmica de materiales cerámicos

    Directory of Open Access Journals (Sweden)

    Barea, R.

    2006-04-01

    Full Text Available Among the different techniques available for measuring thermal conductivity, the comparative method (ASTM E1225-99 is the most appropriate for porous materials. This method determines the thermal conductivity in steady state conditions in the 100 - 1300 K temperature range and for conductivities between 0.2 and 200 W/mK, respectively, depending on the equipment characteristics. In the present paper, the design and development, as well as the start up process, of an apparatus based on this method is described. Thermal conductivity data on a mullite based refractory, measured by both the laser flash and the comparative methods, are presented and comparatively discussed in order to validate the developed equipment.

    Entre las distintas técnicas de medida de la conductividad térmica de materiales, el método comparativo, recogido en la norma ASTM E1225-99, es el más adecuado para muestras porosas o materiales compuestos. Este método permite determinar conductividades térmicas en estado estacionario, en el intervalo de valores de 0,2 a 200 W/mK, y a temperaturas de ensayo en el rango 100-1300 K, dependiendo del diseño del equipo. En este trabajo se expone el diseño, desarrollo y puesta a punto de un equipo de medida de conductividad térmica basado en este método. Paralelamente, se realiza una validación del equipo mediante el estudio comparativo de los resultados obtenidos usando el equipo comparativo y la técnica del pulso láser en materiales porosos de mullita.

  3. Construção de uma fonte de corrente e de uma sonda para medida de condutividade pelo método da sonda de quatro pontas The construction of an eletrical currente source and of a probe for conductivity measurement by a four point probe method

    Directory of Open Access Journals (Sweden)

    Olacir Alves Araújo

    2003-10-01

    Full Text Available This paper describes the construction of an eletrical current source and of a probe to be used in the measurement of eletrical conductivity through a four-point probe method. These pieces of equipments can be obtained at the low price of US$ 50.00 and are adequate for eletrical conductivity measurements in the semiconductor range, that is from 10-1 to 10-6 S cm-1.

  4. Elaboration of Co-60 sources on gilding vyns, with beta efficiencies 80 % or better and its activity measure by the coincidences method; Elaboracion de fuentes de Co-60 sobre vyns dorado, con eficiencias beta del 80 % o mejor y su medida de actividad por el metodo de coincidencias

    Energy Technology Data Exchange (ETDEWEB)

    Cortes P, A.; Tejera R, A.; Becerril V, A.; Ortiz C, A

    1991-12-15

    The measuring technique of radioactive activity by the coincidences method 4 {pi} {beta}{sup -} {gamma} it requires of the elaboration of radioactive sources on thin supports in order to flows the biggest percentage of beta particles, those of the order of the 80 % or but. In this work a procedure for the elaboration of this type of sources, with gilding Vyns is reported. (Author)

  5. Estudo comparativo das medidas manual e digital da fenda palpebral A comparative study of the manual and digital measurements of the palpebral fissure

    Directory of Open Access Journals (Sweden)

    Tânia Pereira Nunes

    2005-12-01

    Full Text Available OBJETIVO: Comparar a medida manual do comprimento vertical da fenda palpebral e sua medida obtida por meio de processamento computadorizado de imagens de indivíduos sem alterações palpebrais. MÉTODOS: Foram analisadas 102 fendas palpebrais de 51 indivíduos normais. A faixa etária variou entre 17 e 84 anos, sendo 25 (49% do sexo masculino e 26 (51% do sexo feminino. RESULTADOS: Não houve diferença estatisticamente significativa entre as medidas manuais da fenda palpebral e as obtidas por meio de processamento computadorizado de imagens. Também não se observou diferença entre ambos os sexos e a faixa etária. CONCLUSÕES: Os resultados obtidos com estes dois métodos podem ser comparados entre si devida à equivalência estatística das medidas.PURPOSE: To compare the measurement of the palpebral fissure done either manually and by computer analysis of image of individuals without lid alterations. METHODS: One hundred two palpebral fissures from 51 normal subjects have been analyzed. The patients' age ranged from 17 to 84 years, with 25 (49% males and 26 (51% females. RESULTS: There was no statistically significant difference between the measurements done either manually or by computer analysis of images, neither, when we grouped the patients by sex or age. Also we did not observe difference between males and females, and regarding age. CONCLUSIONS: The results obtained by both methods are comparable and reliable due to the statistical equivalence of the obtained measurements.

  6. Fundamentos e métodos de medidas de energia dinâmica no SPT

    Directory of Open Access Journals (Sweden)

    Antonio Belincanta

    2000-05-01

    Full Text Available Discutem-se neste trabalho os métodos disponíveis no Brasil e no exterior para a medida da energia transferida às hastes no SPT, apresentando-se inclusive registros típicos dos eventos dinâmicos correspondentes

  7. Ato infracional e medida socioeducativa: representações de adolescentes em L.A.

    Directory of Open Access Journals (Sweden)

    Bianca Izoton Coelho

    2013-01-01

    Full Text Available A pesquisa analisou as representações sociais de ato infracional e medida socioeducativa em adolescentes em conflito com a lei. Utilizou-se, como aporte teórico e metodológico, a Teoria das Representações Sociais. Participaram da pesquisa quarenta e seis adolescentes que cumpriam, há mais de três meses, a medida de Liberdade Assistida. Os dados foram coletados, por meio de entrevistas, com um roteiro semiestruturado e consistiram na técnica das evocações livres por meio dos termos ato infracional e medida socioeducativa. Utilizou-se o software EVOC para análise das evocações emitidas. Os resultados apontaram como caracterização socioeconômica dos adolescentes: renda familiar baixa, baixo nível de escolaridade, local de residência precário, etnias afro-descendentes predominantes e distanciamento da religião. As representações sociais de ato infracional relacionaram-se aos tipos de atos infracionais e às suas causas e consequências. As representações sociais de medida socioeducativa relacionaram-se a ações positivas em que, além da aprendizagem, houve a possibilidade de mudança.

  8. Adolescente em conflito com a lei e medidas socioeducativas: limites e (impossibilidades

    Directory of Open Access Journals (Sweden)

    Francischini, Rosângela

    2005-01-01

    Full Text Available Reinserção social, readaptação, ajustamento social, integração à família e sociedade. Várias são as expressões empregadas para referir-se ao efeito desejado do trabalho com o jovem em conflito com a lei, em cumprimento de medidas socioeducativas, particularmente em privação de liberdade. Parte do aparato legal do Estado, a partir da promulgação do Estatuto da Criança e do Adolescente, significaram, essas medidas, uma chamada à responsabilização do jovens, em face da transgressão cometida. No entanto, avalia-se, o que dá a essas medidas o caráter socioeducativo, fazendo com que o trabalho desenvolvido nas instituições diferencie-se do cumprimento de pena? Estrutura física, formação de recursos humanos, ações educativas e trabalho transdiciplinar são alguns dos aspectos implicados nesta questão. Neste contexto de discussão insere-se o presente artigo, que tem por objetivo discutir o caráter socioeducativo das medidas de privação de liberdade e as possibilidades de reinserção social do jovem em conflito com a lei

  9. Medidas procesales especiales y proteccíon de los derechos humanos Informe general

    NARCIS (Netherlands)

    Vervaele, J.A.E.

    2009-01-01

    El objetivo del informe general es realizar un análisis comparativo de los informes nacionales para evidenciar los procesos de transformación de los sistemas nacionales de justicia penal, en particular del proceso penal, en el que se han introducido medidas procesales especiales para hacer frente al

  10. Rapid detection of atrazine and metolachlor in farm soils: gas chromatography-mass spectrometry-based analysis using the bubble-in-drop single drop microextraction enrichment method.

    Science.gov (United States)

    Williams, D Bradley G; George, Mosotho J; Marjanovic, Ljiljana

    2014-08-06

    Tracking of metolachlor and atrazine herbicides in agricultural soils, from spraying through to harvest, was conducted using our recently reported "bubble-in-drop single-drop microextraction" method. The method showed good linearity (R(2) = 0.999 and 0.999) in the concentration range of 0.01-1.0 ng/mL with LOD values of 0.01 and 0.02 ng/mL for atrazine and metolachlor, respectively. Sonication methods were poor at releasing these herbicides from the soil matrixes, while hot water extraction readily liberated them, providing an efficient accessible alternative to sonication techniques. Good recoveries of 97% and 105% were shown for atrazine and metolachlor, respectively, from the soil. The spiking protocol was also investigated, resulting in a traceless spiking method. We demonstrate a very sensitive technique by which to assess, for example, the length of residence of pesticides in given soils and thus risk of exposure.

  11. Comparação entre o método da gota pendente e o método da gota girante para medida da tensão interfacial entre polímeros Comparison between the pendant drop and spinning drop method to measure interfacial tension between polymers

    Directory of Open Access Journals (Sweden)

    Nicole R. Demarquette

    1997-09-01

    Full Text Available Dois instrumentos, um deles baseado no princípio da gota pendente e o outro baseado no método da gota girante, para medir a tensão interfacial entre polímeros, são apresentados e comparados aqui. Com ambos instrumentos foi possível visualizar a gota de polímero "on line". Os instrumentos mostraram-se complementares quanto às suas áreas de aplicação. O método da gota pendente deve ser usado quando as quantidades de polímero são limitadas ou quando o polímero mais denso é opaco. O método da gota girante deve ser usado quando a degradação térmica pode ser um problema ou quando o polímero menos denso é opaco.In this paper two apparatuses, one based on the principle of the pendant drop method and one based on the principle of the spinning drop are presented and compared. With both apparatuses it was possible to view the drop in real time and calculate the interfacial tension on-line. The two equipments were shown to be complementary in their use. The pendant drop method should be used when there are limited quantities of polymer and/or the denser polymer is opaque. The spinning drop method should be used when thermal degradation could be a problem and/or when the lighter polymer is opaque.

  12. Enrichment and activation of smectite-poor clay

    Energy Technology Data Exchange (ETDEWEB)

    Sarcevica, Inese; Kostjukovs, Juris; Actint, Andris, E-mail: inese.sarcevicha@gmail.com [Department of Chemistry, University of Latvia, Kr. Valdemara street 48, Riga (Latvia)

    2011-06-23

    A new method of smectite clay enrichment has been developed. The method is based on dispersing clay in a phosphate solution and sequential coagulation. The product of enrichment is characterized with X-ray powder diffraction, wavelength dispersive X-ray fluorescence spectrometry, differential thermal analysis and thermogravimetry. Sorption of methylene blue and hexadecylpyridinium bromide on raw and purified clays was studied.

  13. MEDIDAS DE DESEMPENHO E ONTOLOGIAS: UM ESTUDO BIBLIOMÉTRICO PARA IDENTIFICAÇÃO DO USO DE ONTOLOGIAS PARA O SUPORTE DOS PROCESSOS DE MEDIDAS DE DESEMPENHO

    Directory of Open Access Journals (Sweden)

    Vanderlei Freitas Junior

    2015-09-01

    Full Text Available O presente trabalho se propõe analisar as publicações internacionais acerca do emprego das ontologias no contexto de medidas de desempenho. Para isso, foi realizada uma pesquisa bibliométrica em três bases de dados internacionais: Web of Science, Scopus e IEEE. Essa pesquisa foi desenvolvida em duas grandes etapas: (i busca sistemática da literatura, e (ii análise e síntese das informações bibliométricas. Foram localizados 115 trabalhos, escritos por 362 autores vinculados a 84 instituições de 101 países diferentes e estão publicados em 38 periódicos. Os autores identificaram ainda 981 palavras-chave diferentes em seus estudos. Os estudos, de maneira geral, evidenciam a possibilidade de utilização das ontologias como ferramenta semântica em diversas etapas e momentos do processo de medidas de desempenho, especialmente no que tange à representação do conhecimento e elu-cidação dos indicadores chaves, permitindo a interoperabilidade destes indicadores. A partir da identificação destes estudos o presente trabalho contribui para a compreensão das possibilidades de aplicação das ontologias para o suporte aos pro-cessos de medida de desempenho, realizando o mapeamento das publicações inter-nacionais desta temática e evidenciando a possibilidade de representação do conhecimento envolvido nesta tarefa.

  14. Determination of Kr-85 in environmental samples and gaseous effluents from nuclear industries using the standard method; Aplicacion del metodo de concentracion separacion y medida radiactiva por centelleo liquido de Kr-85 en muestras ambientales y en efluentes gaseosos de la industria nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Heras, M c; Perez, M M

    1983-07-01

    The determination of the Kr-85 activity in environmental samples and gaseous effluents from Spanish Nuclear Power Stations is described. The method employed has been published elsewhere. The determinations has been carried out in environmental samples token at JEN Laboratories (Madrid) and the Nuclear Power Stations, Jose Cabrera (Zorita), Garona and Vandellos. Also samples of gaseous effluents of the three plants has been analyzed. Values of the Kr-85 environmental background activity in the Almaraz Nuclear Power Stations, has been determined, before the beginning of its nuclear activity. In this paper the sampling equipment used is described and the values found of Kr-85 activity in all the samples in given. (Author) 29 refs.

  15. Beta activity of enriched uranium

    International Nuclear Information System (INIS)

    Nambiar, P.P.V.J.; Ramachandran, V.

    1975-01-01

    Use of enriched uranium as reactor fuel necessitates its handling in various forms. For purposes of planning and organising radiation protection measures in enriched uranium handling facilities, it is necessary to have a basic knowledge of the radiation status of enriched uranium systems. The theoretical variations in beta activity and energy with U 235 enrichment are presented. Depletion is considered separately. Beta activity build up is also studied for two specific enrichments, in respect of which experimental values for specific alpha activity are available. (author)

  16. Abuso de las medidas y medidas abusivas. Crítica al pensamiento bibliométrico hegemónico

    OpenAIRE

    GÒMEZ-MORALES, YURI JACK

    2018-01-01

    RESUMEN En este ensayo reflexivo se hace una crítica a la economía política de la medición de la ciencia, la cual se ejemplifica mediante el análisis del factor de impacto como medida de la calidad científica; se explican los mecanismos sociales y culturales que operan en la construcción de estas formas de pensar y actuar hegemónicas; y se advierte sobre las graves consecuencias de este proyecto global para la desinstitucionalización de la ciencia local, y el surgimiento de una ciencia nacion...

  17. Uranium enrichment by gas centrifuge

    International Nuclear Information System (INIS)

    Heriot, I.D.

    1988-01-01

    After recalling the physical principles and the techniques of centrifuge enrichment the report describes the centrifuge enrichment programmes of the various countries concerned and compares this technology with other enrichment technologies like gaseous diffusion, laser, aerodynamic devices and chemical processes. The centrifuge enrichment process is said to be able to replace with advantage the existing enrichment facilities in the short and medium term. Future prospects of the process are also described, like recycled uranium enrichment and economic improvements; research and development needs to achieve the economic prospects are also indicated. Finally the report takes note of the positive aspect of centrifuge enrichment as far as safeguards and nuclear safety are concerned. 27 figs, 113 refs

  18. Data analysis for neutron monitoring in an enrichment facility

    International Nuclear Information System (INIS)

    Markin, J.T.; Stewart, J.E.; Goldman, A.S.

    1982-01-01

    Area monitoring of neutron radiation to detect high-enriched uranium production is a potential strategy for inspector verification of operations in the cascade area of a centrifuge enrichment facility. This paper discusses the application of statistical filtering and hypothesis testing procedures to experimental data taken in an enrichment facility. The results demonstrate that these data analysis methods can enhance detection of facility misoperation by neutron monitoring

  19. Online trapping and enrichment ultra performance liquid chromatography-tandem mass spectrometry method for sensitive measurement of 'arginine-asymmetric dimethylarginine cycle' biomarkers in human exhaled breath condensate

    Energy Technology Data Exchange (ETDEWEB)

    Di Gangi, Iole Maria, E-mail: giordano@pediatria.unipd.it [Chromatography and Mass Spectrometry Laboratory, Department of Women' s and Children' s Health, University of Padova (Italy); Pirillo, Paola [Chromatography and Mass Spectrometry Laboratory, Department of Women' s and Children' s Health, University of Padova (Italy); Carraro, Silvia [Unit of Allergy and Respiratory Diseases, Department of Women' s and Children' s Health, University of Padova (Italy); Gucciardi, Antonina; Naturale, Mauro [Chromatography and Mass Spectrometry Laboratory, Department of Women' s and Children' s Health, University of Padova (Italy); Baraldi, Eugenio [Unit of Allergy and Respiratory Diseases, Department of Women' s and Children' s Health, University of Padova (Italy); Giordano, Giuseppe [Chromatography and Mass Spectrometry Laboratory, Department of Women' s and Children' s Health, University of Padova (Italy)

    2012-11-19

    Highlights: Black-Right-Pointing-Pointer Simultaneous quantification of 'arginine-ADMA cycle' metabolites developed in EBC. Black-Right-Pointing-Pointer EBC is a non-invasive matrix highly useful in patients with respiratory diseases. Black-Right-Pointing-Pointer Method, fast, precise and accurate, is suitable in the pediatric clinical studies. Black-Right-Pointing-Pointer Sensitivity is increased using on-line trapping and enrichment-UPLC-MS/MS method. Black-Right-Pointing-Pointer EBC measurements in asthmatic adolescents confirm that ADMA is increased in asthma. - Abstract: Background: Exhaled breath condensate (EBC) is a biofluid collected non invasively that, enabling the measurement of several biomarkers, has proven useful in the study of airway inflammatory diseases, including asthma, COPD and cystic fibrosis. To the best of our knowledge, there is no previous report of any analytical method to detect ADMA in EBC. Objectives: Aim of this work was to develop an online sample trapping and enrichment system, coupled with an UPLC-MS/MS method, for simultaneous quantification of seven metabolites related to 'Arginine-ADMA cycle', using the isotopic dilution. Methods: Butylated EBC samples were trapped in an online cartridge, washed before and after each injection with cleanup solution to remove matrix components and switched inline into the high pressure analytical column. Multiple reaction monitoring in positive mode was used for analyte quantification by tandem mass spectrometry. Results: Validation studies were performed in EBC to examine accuracy, precision and robustness of the method. For each compound, the calibration curves showed a coefficient of correlation (r{sup 2}) greater than 0.992. Accuracy (%Bias) was <3% except for NMMA and H-Arg (<20%), intra- and inter-assay precision (expressed as CV%) were within {+-}20% and recovery ranged from 97.1 to 102.8% for all analytes. Inter-day variability analysis on 20 EBC of adult subjects did

  20. The world enrichment market

    International Nuclear Information System (INIS)

    Gunter, L.; McCants, C.; Rutkowski, E.

    1991-01-01

    The enrichment market can be divided into two periods: the near-term market (1991 to 1995) and the long-term market (1995 and beyond). The near-term market is characterized by limited unfilled requirements of 4% per year, to be supplied by national stockpiles and excess inventories. This low-cost material will be drawn down by about 1993, causing a subsequent price rise. As the price rises, primary supplier activity is expected to increase. In the near-term, two contracting activities are apparent: spot; and intermediate-term. The current spot market is expected to last until available low cost inventories are drawn down. Recently, in attempts to gain market share, suppliers have offered attractively priced intermediate-term (3 year) contracts for 1996 to 1998. While a small spot market will continue after 1995, it is anticipated that utilities will prefer a mix of medium- and long-term (5 to 10 year) contracts from primary suppliers for most of their enrichment requirements. As national stockpiles and utility inventories are consumed, low-cost supply available to the spot market is expected to diminish. Consequently, with little low-cost supply available, the only apparent source of material will be from primary suppliers, and the resulting competition over market share is expected to be intense. (author)

  1. Motif enrichment tool.

    Science.gov (United States)

    Blatti, Charles; Sinha, Saurabh

    2014-07-01

    The Motif Enrichment Tool (MET) provides an online interface that enables users to find major transcriptional regulators of their gene sets of interest. MET searches the appropriate regulatory region around each gene and identifies which transcription factor DNA-binding specificities (motifs) are statistically overrepresented. Motif enrichment analysis is currently available for many metazoan species including human, mouse, fruit fly, planaria and flowering plants. MET also leverages high-throughput experimental data such as ChIP-seq and DNase-seq from ENCODE and ModENCODE to identify the regulatory targets of a transcription factor with greater precision. The results from MET are produced in real time and are linked to a genome browser for easy follow-up analysis. Use of the web tool is free and open to all, and there is no login requirement. ADDRESS: http://veda.cs.uiuc.edu/MET/. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  2. Phosphopeptide enrichment by immobilized metal affinity chromatography

    DEFF Research Database (Denmark)

    Thingholm, Tine E.; Larsen, Martin R.

    2016-01-01

    Immobilized metal affinity chromatography (IMAC) has been the method of choice for phosphopeptide enrichment prior to mass spectrometric analysis for many years and it is still used extensively in many laboratories. Using the affinity of negatively charged phosphate groups towards positively...... charged metal ions such as Fe3+, Ga3+, Al3+, Zr4+, and Ti4+ has made it possible to enrich phosphorylated peptides from peptide samples. However, the selectivity of most of the metal ions is limited, when working with highly complex samples, e.g., whole-cell extracts, resulting in contamination from...

  3. Enrichment and Preservation of Architectural Knowledge

    DEFF Research Database (Denmark)

    Beetz, Jakob; Blümel, Ina; Dietze, Stefan

    2016-01-01

    In the context of the EU FP7 DURAARK project (2013–2016), inter-disciplinary methods, technologies and tools have been researched and developed, that support the Long Term Preservation of semantically enriched digital representations of built structures. The results of the research efforts include...... approaches of semi-automatically deriving building models from point cloud data sets acquired from laser scans and the integration and overlay of such representations with explicit Building Information Models (BIM). We introduce novel ways for the further semantic enrichment of such hybrid building models...

  4. A consideration on laser enrichment module

    International Nuclear Information System (INIS)

    Arisawa, Takashi; Shiba, Koreyuki

    1983-09-01

    Several problems are discussed for designing a simplified enrichment module based on Atomic Laser Isotope Separation Method, which involve the vaporization of metal, laser excitation, laser ionization and ion recovery. The conditions at which the consumed energy has the minimum value are obtained by calculating the specific energy consumption for producing unit amount of enriched products. It is found that there should be an appropriate relationship between the processing atomic density and the electrode gap in order to avoid the enrichment loss caused by the charge exchange during the ion recovery. Moreover it is also found that this relation depends on the electrode length measured along both the atomic beam direction and the laser beam direction. (author)

  5. Modular enrichment measurement system for in-situ enrichment assay

    International Nuclear Information System (INIS)

    Stewart, J.P.

    1976-01-01

    A modular enrichment measurement system has been designed and is in operation within General Electric's Nuclear Fuel Fabrication Facility for the in-situ enrichment assay of uranium-bearing materials in process containers. This enrichment assay system, which is based on the ''enrichment meter'' concept, is an integral part of the site's enrichment control program and is used in the in-situ assay of the enrichment of uranium dioxide (UO 2 ) powder in process containers (five gallon pails). The assay system utilizes a commercially available modular counting system and a collimnator designed for compatability with process container transport lines and ease of operator access. The system has been upgraded to include a microprocessor-based controller to perform system operation functions and to provide data acquisition and processing functions. Standards have been fabricated and qualified for the enrichment assay of several types of uranium-bearing materials, including UO 2 powders. The assay system has performed in excess of 20,000 enrichment verification measurements annually and has significantly contributed to the facility's enrichment control program

  6. Mitoxantrone Loaded Superparamagnetic Nanoparticles for Drug Targeting: A Versatile and Sensitive Method for Quantification of Drug Enrichment in Rabbit Tissues Using HPLC-UV

    Directory of Open Access Journals (Sweden)

    Rainer Tietze

    2010-01-01

    Full Text Available In medicine, superparamagnetic nanoparticles bound to chemotherapeutics are currently investigated for their feasibility in local tumor therapy. After intraarterial application, these particles can be accumulated in the targeted area by an external magnetic field to increase the drug concentration in the region of interest (Magnetic-Drug-Targeting. We here present an analytical method (HPLC-UV, to detect pure or ferrofluid-bound mitoxantrone in a complex matrix even in trace amounts in order to perform biodistribution studies. Mitoxantrone could be extracted in high yields from different tissues. Recovery of mitoxantrone in liver tissue (5000 ng/g was 76±2%. The limit of quantification of mitoxantrone standard was 10 ng/mL ±12%. Validation criteria such as linearity, precision, and stability were evaluated in ranges achieving the FDA requirements. As shown for pilot samples, biodistribution studies can easily be performed after application of pure or ferrofluid-bound mitoxantrone.

  7. Enrichment of boron 10

    International Nuclear Information System (INIS)

    Coutinho, C.M.M.; Rodrigues Filho, J.S.R.; Umeda, K.; Echternacht, M.V.

    1990-01-01

    A isotopic separation pilot plant with five ion exchange columns interconnected in series were designed and built in the IEN. The columns are charged with a strong anionic resin in its alkaline form. The boric acid solution is introduced in the separation columns until it reaches a absorbing zone length which is sufficient to obtain the desired boron-10 isotopic concentration. The boric acid absorbing zone movement is provided by the injection of a diluted hydrochloric acid solution, which replace the boric acid throughout the columns. The absorbing zone equilibrium length is proportional to its total length. The enriched boron-10 and the depleted boron are located in the final boundary and in the initial position of the absorbing zones, respectively. (author)

  8. Advanced Neutron Source enrichment study

    International Nuclear Information System (INIS)

    Bari, R.A.; Ludewig, H.; Weeks, J.R.

    1996-01-01

    A study has been performed of the impact on performance of using low-enriched uranium (20% 235 U) or medium-enriched uranium (35% 235 U) as an alternative fuel for the Advanced Neutron Source, which was initially designed to use uranium enriched to 93% 235 U. Higher fuel densities and larger volume cores were evaluated at the lower enrichments in terms of impact on neutron flux, safety, safeguards, technical feasibility, and cost. The feasibility of fabricating uranium silicide fuel at increasing material density was specifically addressed by a panel of international experts on research reactor fuels. The most viable alternative designs for the reactor at lower enrichments were identified and discussed. Several sensitivity analyses were performed to gain an understanding of the performance of the reactor at parametric values of power, fuel density, core volume, and enrichment that were interpolations between the boundary values imposed on the study or extrapolations from known technology

  9. Medidas de experiencia e cienciometria para avaliar impacto da producao cientifica

    Directory of Open Access Journals (Sweden)

    Marco Akerman

    2013-08-01

    Full Text Available Propõe-se a “experienciometria” como medida da experiência acadêmica de pesquisadores de modo a complementar a medida consagrada da cienciometria nas avaliações de competências em pesquisa. O artigo baseia-se em parte na produção cientifica do autor, utilizada sob uma perspectiva qualitativa para explorar possibilidades de análise de impactos. Ensaia-se uma síntese com possíveis implicações dessa análise, indicando algumas possibilidades para se avaliar impacto de pesquisas e artigos que não se restrinjam exclusivamente à contagem de números de citações dos autores para o cálculo do fator de impacto.

  10. La medida de la inteligencia emocional en el ámbito psicoeducativo

    Directory of Open Access Journals (Sweden)

    Iratxe Antonio-Agirre

    2017-01-01

    Full Text Available La diversidad individual que alberga la escuela representa un reto ineludible. Para promover el desarrollo integral del alumnado resulta necesario favorecer competencias para la vida, como la inteligencia emocional, que les ayuden a lograr un mejor ajuste sociopersonal y escolar. Sin embargo, la diversidad conceptual en torno a la inteligencia emocional ha contribuido a la proliferación de medidas y ha dificultado la integración de la investigación. En todo caso, atendiendo a las propiedades psicométricas y a las dimensiones que miden, este trabajo recoge los principales instrumentos de medida de la inteligencia emocional que posibilitan una evaluación más rigurosa y exhaustiva del constructo en niños, niñas y adolescentes.

  11. Medida de la eficiencia ambiental en la industria cerámica española

    Directory of Open Access Journals (Sweden)

    Sala Garrido, Ramon

    2008-01-01

    Full Text Available En el trabajo de Färe, Grosskopf y Hernández-Sancho (2004 se propone un índice de eficiencia medioambiental, para medir el grado en el que las empresas generan outputs deseables al mismo tiempo que reducen su outputs no deseables. Este indicador consiste en una ratio entre una medida para outputs deseables y otra para no deseables. Cada uno de ellos está basado en el uso de las funciones distancia. El índice de los outputs deseables mide el éxito de una empresa k en aumentar estos outputs utilizando el mismo número de inputs y generando el mismo nivel de outputs no deseables. Esta medida de eficiencia medioambiental se ha aplicado a la industria de los pavimentos cerámicos sobre una muestra de empresas a las que se ha aplicado un test de Kruskal-Wallis para determinar la utilidad de este indicador.

  12. TAM: a method for enrichment and depletion analysis of a microRNA category in a list of microRNAs.

    Science.gov (United States)

    Lu, Ming; Shi, Bing; Wang, Juan; Cao, Qun; Cui, Qinghua

    2010-08-09

    MicroRNAs (miRNAs) are a class of important gene regulators. The number of identified miRNAs has been increasing dramatically in recent years. An emerging major challenge is the interpretation of the genome-scale miRNA datasets, including those derived from microarray and deep-sequencing. It is interesting and important to know the common rules or patterns behind a list of miRNAs, (i.e. the deregulated miRNAs resulted from an experiment of miRNA microarray or deep-sequencing). For the above purpose, this study presents a method and develops a tool (TAM) for annotations of meaningful human miRNAs categories. We first integrated miRNAs into various meaningful categories according to prior knowledge, such as miRNA family, miRNA cluster, miRNA function, miRNA associated diseases, and tissue specificity. Using TAM, given lists of miRNAs can be rapidly annotated and summarized according to the integrated miRNA categorical data. Moreover, given a list of miRNAs, TAM can be used to predict novel related miRNAs. Finally, we confirmed the usefulness and reliability of TAM by applying it to deregulated miRNAs in acute myocardial infarction (AMI) from two independent experiments. TAM can efficiently identify meaningful categories for given miRNAs. In addition, TAM can be used to identify novel miRNA biomarkers. TAM tool, source codes, and miRNA category data are freely available at http://cmbi.bjmu.edu.cn/tam.

  13. Expansion of U. S. uranium enrichment capacity. Final environmental statement

    Energy Technology Data Exchange (ETDEWEB)

    1976-04-01

    Reasonably foreseeable environmental, social, economic, and technological costs and benefits of postulated expansion of U. S. enrichment capacity through the year 2000 and reasonably available alternatives to such expansion are described. Both the gas centrifuge and gaseous diffusion methods for the enrichment of uranium are considered in this impact assessment. (JGB)

  14. Medidas de aislamiento en enfermedades transmisibles dentro del ámbito hospitalario

    OpenAIRE

    Villanueva Rábano, Raúl

    2016-01-01

    En el ámbito hospitalario, son un problema frecuente las infecciones nosocomiales, causadas por las enfermedades transmisibles que pasan de unos pacientes a otros. Estas infecciones son potencialmente prevenibles, a través de medidas de aislamiento llevadas a cabo por el personal sanitario y no sanitario encargado de atender al paciente. En este aspecto, el papel del personal de enfermería es de fundamental importancia dado que mantienen un estrecho contacto con el paciente como cuidadores pr...

  15. CONTROL EJECUTIVO Y FLUIDEZ VERBAL EN POBLACIÓN INFANTIL: MEDIDAS CUANTITATIVAS, CUALITATIVAS Y TEMPORALES

    Directory of Open Access Journals (Sweden)

    JULIÁN MARINO

    2011-01-01

    Full Text Available Para evaluar la evocación de palabras asociadas bajo una consigna de prueba de fluidez verbal (PFV se tienen en cuenta la cantidad de palabras producidas, las asociaciones categoriales y fonológicas entre ellas y el tiempo en que son evocadas. A continuación se presenta un estudio que se realizó aplicando PFV semánticas y fonológicas en una población infantil (8 - 12 años de la ciu dad de Granada (España, que tuvo por objetivos: (1 revisar las tradicionales medidas en PFV, (2 actualizar los índices temporales de medición y (3 presentar combinaciones de éstas con el fin de establecer con mayor precisión la participación del control ejecutivo en PFV. Se encontraron resultados significativos entre los que se destaca que la combinación entre la medida temporal de evocación de palabras conjuntas y la cualitativa de palabras en relación cluster podría aislar con mayor fiabilidad el compromiso de funciones ejecutivas como el control inhibitorio y la flexibilidad cognitiva. Esto se determinó empleando medidas de regresión, ingre sando como variables independientes funciones ejecutivas y como variables dependientes cada una de las medidas de Fluidez Verbal. Para la mencionada combinación de palabras conjuntas - palabras en relación cluster (PFV fonológica letra P se halló un R2 de .41 (p = .000 ingresando como variables predictoras el Trail Making B y la parte Color - Palabra de la versión Trenerry del paradigma Stroop (Lezak, 1995. Se describen también otras posibilidades de medición temporales - cualitativas y su posible utilidad clínica y de investigación.

  16. Medidas de bioseguridad adoptadas en el manejo con materiales biológicos en Laboratorios Liorad

    Directory of Open Access Journals (Sweden)

    Nancy Burguet Lago

    2013-03-01

    Full Text Available Introducción: el trabajo con microorganismos puede conllevar a riesgos tanto para el personal que trabaja con los mismos como para el medio ambiente. La existencia de laboratorios de seguridad biológica y la implementación de medidas en la manipulación de los agentes biológicos minimizan el riesgo. Objetivo: evaluar las medidas de bioseguridad adoptadas en el manejo con materiales biológicos en Laboratorios Liorad. Métodos: empleo de una lista de chequeo y análisis de los resultados a través de una Matriz DAFO para valorar si el diseño de la instalación cumple con la bioseguridad. Además establecer un sistema documental para la manipulación de microorganismos y la confección de un plan de capacitación para el personal que trabaja en el laboratorio de control microbiológico. Resultados: la lista de chequeo permitió identificar como principal debilidad el no disponer de un doble pasillo para el traslado del material limpio y sucio. Como fortalezas, cumplir con las prácticas y procesamientos adecuados y el contar con equipos de seguridad biológica. El sistema documental incorporó a los procedimientos establecidos para la manipulación, un acápite referido a la «Peligrosidad y Medidas de Seguridad». El programa de capacitación desarrollado permitió proveer conocimientos específicos referidos a esta temática. Conclusión: las medidas adoptadas en el laboratorio permiten plantear que de manera general se cumplen los requisitos establecidos en materia de Bioseguridad para el trabajo con microorganismos.

  17. Medidas Explícitas e Implícitas de las Actitudes Hacia las Mujeres

    Directory of Open Access Journals (Sweden)

    Manuel Cárdenas

    2009-01-01

    Full Text Available El presente estudio tiene como objeto utilizar el IAT (Implicit Association Test para medir actitudes hacia las mujeres, de modo de comparar dichas evaluaciones de carácter automático con las obtenidas por medio de una medida explícita. Además, los participantes respondieron a un inventario de roles sexuales de modo de ver si los sujetos manejaban el estereotipo dominante asociado a lo femenino y sus efectos sobre el prejuicio. La muestra quedó conformada por 80 estudiantes universitarios. No se detectaron diferencias significativas entre hombres y mujeres en los estereotipos asociados a la mujer, ni en la evaluación que se hace de estas (medida explícita. En la medida de IAT las diferencias entre sexos aparecen, indicando que los hombres muestran una actitud implícita más negativa hacia las mujeres. De este modo se confirma que la aparente disminución del prejuicio que puede constatarse en los cuestionarios podría encubrir el hecho de que las actitudes negativas se mantienen a nivel implícito.

  18. Violência Juvenil e Medidas Socioeducativas: Revisão de Literatura

    Directory of Open Access Journals (Sweden)

    Jacqueline de Oliveira Moreira

    2017-10-01

    Full Text Available RESUMO Neste artigo, partindo do objetivo de compreender a relação entre violência juvenil e a resposta do Estado através das medidas socioeducativas, buscamos explicitar o pano de fundo das pesquisas científicas atuais. Realizamos uma meta-análise dos artigos publicados sobre adolescência e medidas socioeducativas, no campo da psicologia, entre os anos de 2000 e 2012. Concluímos que essa produção nacional pode ser reunida em três grandes categorias: (a prático-experiencial, que apresenta relatos analíticos da prática na aplicação das medidas socioeducativas, privilegiando a experiência concreta; (b político-institucional, que discute criticamente a dimensão política da lógica socioeducativa, detendo-se na análise estrutural de sua legislação e de suas instituições; (c sociocultural, que analisa os determinantes sociais e psíquicos do fenômeno da violência juvenil.

  19. Medidas cautelares innominadas en la protección de los derechos de autor y conexos en Colombia

    Directory of Open Access Journals (Sweden)

    Nixon Jefrey Gil Aguirre

    2016-12-01

    Full Text Available Actualmente, el ejercicio de funciones jurisdiccionales atribuidas a las autoridades administrativas ha generado interpretaciones judiciales confusas, en la aplicación de medidas cautelares innominadas para la protección de derechos de autor y conexos en Colombia; así lo evidencia el análisis realizado a las medidas cautelares descritas en la Ley 1493 del año 2011, que otorgó competencias de inspección, vigilancia y control sobre las sociedades de gestión colectiva de derechos de autor y conexos; en este sentido se requiere describir las funciones jurisdiccionales de la Dirección Nacional de Derecho de Autor respecto a las medidas cautelares y su importancia en la protección de los derechos de autor y conexos, al igual que analizar las medidas cautelares innominadas desde su origen, las características y lo propuesto por la Corte Constitucional en materia de funciones jurisdiccionales de la Dirección Nacional de Derecho de Autor y respecto de la procedencia de las medidas cautelares innominadas como competencia de esta entidad. Se concluye que las medidas cautelares nominadas no son suficientes, y que se hace necesario acudir a las medidas cautelares innominadas, como herramienta jurídica del funcionario administrativo, sin que ello implique arbitrariedad y prejuzgamiento en el proceso.

  20. Enrichment plants. A survey of major new uranium enriching projects

    International Nuclear Information System (INIS)

    Kovan, D.

    1976-01-01

    The work enrichment situation is reported. The development of enrichment in the U.S. and in Europe is outlined. A brief description is given of the technology of separation by diffusion and by centrifugation and the advantages and disadvantages of the two processes are compared. Finally the supply and demand situation is briefly considered. (U.K.)

  1. Enrichment technology. Dependable vendor of gas centrifuges; Enrichment Technology. Zuverlaessiger Lieferant von Gaszentrifugen

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2011-10-15

    Enrichment Technology is an innovative, high-tech company that develops, manufactures and installs gas centrifuges for enriching uranium. In addition, Enrichment Technology designs enrichment plants that use gas centrifuge technology. This technology offers the most efficient and cost-effective method for enriching uranium yet: high-performance, safe technology that dominates the market with a global share of 45 percent. A determining factor in Enrichment Technology's success is its mission: supplying its customers with safe, reliable technology. Production of the centrifuges requires versatile know-how and collaboration between different departments as well as interdisciplinary teams at the various sites. More than 2000 operators at 8 sites in 5 countries contribute their individual knowledge and personal skills in order to produce this exceptional technology. The head office is in Beaconsfield near London and the operational headquarters are in Almelo in the Netherlands. There are other sites in Germany (Juelich und Gronau), Great Britain (Capenhurst) as well as project sites in the USA and France. Capenhurst is where experienced engineers design new enrichment plants and organise their construction. Centrifuge components are manufactured in Almelo and Juelich, while the pipework needed to connect up the centrifuges is produced at the site in Gronau. In Juelich, highly qualified scientists in interdisciplinary teams are continuously researching ways of improving the current centrifuges. Communication between specialists in the fields of chemistry, physics and engineering forms the basis for the company's success and the key to extending this leading position in the global enrichment market. (orig.)

  2. Dois pesos e duas medidas: uma proposta para discutir a natureza do sistema de unidades de medida na sala de aula

    Directory of Open Access Journals (Sweden)

    Lidiany C. de O. Godoi

    2009-01-01

    Full Text Available http://dx.doi.org/10.5007/2175-7941.2008v25n3p523 Sugerimos neste artigo um plano de ensino que, envolvendo um trabalho interdisciplinar, discuta a natureza do Sistema de Unidades de Medida. Por meio de dinâmicas de grupos, leitura e interpretação de textos históricos, poemas e ilustrações e também através de outras fontes, acreditamos ser possível mostrar que o sistema de unidades de medida adotado atualmente (SI não é um dado natural, mas o resultado de um processo histórico de negociação de significados e, portanto, contingente das relações sociais. A partir da perspectiva dos Estudos Sociais da Ciência (ou Nova História e Sociologia do Conhecimento, apresentamos algumas discussões que envolveram a adoção do Sistema Decimal na França, bem como as dificuldades de sua posterior implementação no Brasil, expressa pela Revolta do Quebra-Quilos. Esperamos que as dinâmicas sugeridas neste trabalho possibilitem aos estudantes reconhecerem a si mesmos como participantes do processo de construção do conhecimento científico através da observação diária das mudanças que conferem novas feições a ele e também da tentativa de tornar mais significativo um tópico do currículo que, muitas vezes, é considerado desinteressante, enfadonho ou simples memorização de grandezas e de suas conversões.

  3. Laser enrichment: a new path to proliferation

    International Nuclear Information System (INIS)

    Casper, B.M.

    1977-01-01

    The use of lasers to obtain enriched uranium is an easier and cheaper method than methods currently in use. The immediate concern is that it could promote easy access to nuclear weapons by countries that do not presently have them. Mr. Casper feels that the U.S. government is working against itself; while the State Department is seeking to block one path to proliferation, ERDA laboratories are developing new technology that could open another. The proliferation implications have not been factored in a serious way into the decisions to proceed with this research. It is also clear that the United States does not now have a comprehensive policy that deals with all potentially important paths to proliferation, including laser enrichment. Mr. Casper states that there is still time to stop and consider whether laser enrichment should be developed, in light of its broader consequences. But this will not happen if the decisions are left exclusively in the hands of those promoting the technology, the author says. It is just this sort of situation that prompted the creation of several government institutions to provide independent assessments of new technologies. The Office of Technology Assessment, the Nuclear Regulatory Commission, and the Arms Control and Disarmament Agency all have the authority to intervene. Laser enrichment provides a good test of these institutions and of the viability of the concept of technology assessment. The status, benefits and risks, and the policy needed on laser research are discussed

  4. Medida del coeficiente d33 de piezocomposites por interferometría láser

    Directory of Open Access Journals (Sweden)

    Montero de Espinosa, F.

    2002-02-01

    Full Text Available The measurement of piezoelectric coefficient d33 is made using devices like the Berlincourt Meter ®, based on a force generator internally calibrated by a reference piezoelectric element which measures the charge ratio Culomb/Newton. For estructures such as piezoelectric composites, formed by materials of very different elastic properties, the measurements made by the described system present a great dispersión which depends on both the size of the pressing head and the aplied arm pressure. This work presents a set of systematic measurements of d33 for several piezocomposite geometries by means of optical interferometry. These measurements are compared with those obtained with the Berlincourt. It is observed that both techniques render closer results as the net step is reduced.La medida del coeficiente piezoeléctrico d33 se realiza utilizando equipos como el Berlincourt Meter ®, basados en un generador de fuerza calibrado internamente por un elemento piezoeléctrico de referencia que mide la relación de carga, Culombio/ Newton. Estos sistemas están absolutamente contrastados, tomando los grupos de investigación sus medidas como estándar. En el caso de estructuras tales como los composites piezoeléctricos, en los que la estructura está formada por materiales de muy distinta elasticidad, bien por este hecho, bien por la misma estructura, las medidas con el sistema descrito presentan una gran dispersión dependiendo del tamaño de la cabeza presionante y la presión del brazo. Dado que en el modelado de resonadores piezocomposite para su uso en aplicaciones con acoplamiento en aire es necesario conocer dicho parámetro sin incertidumbre, se presentan en el trabajo una serie de medidas sistemáticas de dicho coeficiente para varias geometrías de piezocomposites utilizando interferometría óptica. Se relaciona así mismo estas medidas con el resultado obtenido con el equipo estándar antes referido observando como cuanto más pequeño es

  5. Evaluating biomarkers for prognostic enrichment of clinical trials.

    Science.gov (United States)

    Kerr, Kathleen F; Roth, Jeremy; Zhu, Kehao; Thiessen-Philbrook, Heather; Meisner, Allison; Wilson, Francis Perry; Coca, Steven; Parikh, Chirag R

    2017-12-01

    A potential use of biomarkers is to assist in prognostic enrichment of clinical trials, where only patients at relatively higher risk for an outcome of interest are eligible for the trial. We investigated methods for evaluating biomarkers for prognostic enrichment. We identified five key considerations when considering a biomarker and a screening threshold for prognostic enrichment: (1) clinical trial sample size, (2) calendar time to enroll the trial, (3) total patient screening costs and the total per-patient trial costs, (4) generalizability of trial results, and (5) ethical evaluation of trial eligibility criteria. Items (1)-(3) are amenable to quantitative analysis. We developed the Biomarker Prognostic Enrichment Tool for evaluating biomarkers for prognostic enrichment at varying levels of screening stringency. We demonstrate that both modestly prognostic and strongly prognostic biomarkers can improve trial metrics using Biomarker Prognostic Enrichment Tool. Biomarker Prognostic Enrichment Tool is available as a webtool at http://prognosticenrichment.com and as a package for the R statistical computing platform. In some clinical settings, even biomarkers with modest prognostic performance can be useful for prognostic enrichment. In addition to the quantitative analysis provided by Biomarker Prognostic Enrichment Tool, investigators must consider the generalizability of trial results and evaluate the ethics of trial eligibility criteria.

  6. Utilizing ion-pairing hydrophilic interaction chromatography solid phase extraction for efficient glycopeptide enrichment in glycoproteomics

    DEFF Research Database (Denmark)

    Mysling, Simon; Palmisano, Giuseppe; Højrup, Peter

    2010-01-01

    Glycopeptide enrichment is a prerequisite to enable structural characterization of protein glycosylation in glycoproteomics. Here we present an improved method for glycopeptide enrichment based on zwitter-ionic hydrophilic interaction chromatography solid phase extraction (ZIC-HILIC SPE...

  7. Industrial aspects in uranium enrichment

    International Nuclear Information System (INIS)

    Mezin, M.

    1982-05-01

    Characteristics of isotope separation processes in operation and under development are discussed. These include the number of stages in series, the number of components, the component unit capacity and enery requirements. The implementation of an enrichment process and the question of an enrichment plant in Australia are also considered

  8. AEC determines uranium enrichment policy

    International Nuclear Information System (INIS)

    Anon.

    1992-01-01

    The Advisory Committee on Uranium Enrichment of the Atomic Energy Commission (AEC) has submitted a report to AEC chairman concerning the promotion of the introduction of advanced material, high performance centrifuges to replace conventional metallic drum centrifuges, and the development of next generation advanced centrifuges. The report also called for the postponement until around 1997 of the decision whether the development should be continued or not on atomic vapor laser isotope separation (AVLIS) and molecular laser isotope separation (MLIS) processes, as well as the virtual freezing of the construction of a chemical process demonstration plant. The report was approved by the AEC chairman in August. The uranium enrichment service market in the world will continue to be characterized by oversupply. The domestic situation of uranium enrichment supply-demand trend, progress of the expansion of Rokkasho enrichment plant, the trend in the development of gas centrifuge process and the basic philosophy of commercializing domestic uranium enrichment are reported. (K.I.)

  9. Uranium enrichment: an activity for the riches

    International Nuclear Information System (INIS)

    Mundim, S.G.

    1985-01-01

    The United States in the first place, the European countries next, have begun to maintain strict control of the technology transfer of the methods of enrichment, helped largely by the International Atomic Energy Agency which creates additional obstacles with is so-called 'safeguards'. So that it can possess a complete energy cycle, Brazil needs to have access to the techniques of enrichment. The methods are many and still extremely costly. Committed to signing a risk contract with Germany in the scope of its nuclear treaty with the intention of implementing here the jetcentrofuge method, Brazil should, nevertheless, make an effort to cause to emerge from its laboratories a new procedure which would permit it to become independent in this sector. (Author) [pt

  10. New generation enrichment monitoring technology for gas centrifuge enrichment plants

    International Nuclear Information System (INIS)

    Ianakiev, Kiril D.; Alexandrov, Boian S.; Boyer, Brian D.; Hill, Thomas R.; Macarthur, Duncan W.; Marks, Thomas; Moss, Calvin E.; Sheppard, Gregory A.; Swinhoe, Martyn T.

    2008-01-01

    The continuous enrichment monitor, developed and fielded in the 1990s by the International Atomic Energy Agency, provided a go-no-go capability to distinguish between UF 6 containing low enriched (approximately 4% 235 U) and highly enriched (above 20% 235 U) uranium. This instrument used the 22-keV line from a 109 Cd source as a transmission source to achieve a high sensitivity to the UF 6 gas absorption. The 1.27-yr half-life required that the source be periodically replaced and the instrument recalibrated. The instrument's functionality and accuracy were limited by the fact that measured gas density and gas pressure were treated as confidential facility information. The modern safeguarding of a gas centrifuge enrichment plant producing low-enriched UF 6 product aims toward a more quantitative flow and enrichment monitoring concept that sets new standards for accuracy stability, and confidence. An instrument must be accurate enough to detect the diversion of a significant quantity of material, have virtually zero false alarms, and protect the operator's proprietary process information. We discuss a new concept for advanced gas enrichment assay measurement technology. This design concept eliminates the need for the periodic replacement of a radioactive source as well as the need for maintenance by experts. Some initial experimental results will be presented.

  11. Effects of Enrichment Presentation and Other Factors on Behavioral Welfare of Pantropical Spotted Dolphin (Stenella attenuata).

    Science.gov (United States)

    Perez, Barbara C; Mehrkam, Lindsay R; Foltz, Amanda R; Dorey, Nicole R

    2018-01-01

    Environmental enrichment is a crucial element of promoting welfare for animals in captivity. However, enrichment programs are not always formally evaluated for their efficacy. Furthermore, there is little empirical evidence of enrichment evaluation for species of small cetaceans in zoological settings. A wide range of variables may potentially influence enrichment efficacy and how it in turn affects behavior. The purpose of this study was to determine the most preferred environmental enrichment, and method of presentation, for a species that has not been well studied in captivity, the pantropical spotted dolphin (Stenella attenuata). In order to determine which enrichment items and method of presentation were most effective at eliciting enrichment interaction, we systematically examined how several variables of enrichment influenced enrichment interaction. The results suggested that presenting enrichment after training sessions influenced interaction with the enrichment. The results also indicated preference for enrichment type and a specific enrichment device. Finally, factors that influenced interaction were also found to influence aberrant behavior. The results support the premise that enrichment be "redefined" for each species and each individual.

  12. Optimal pin enrichment distributions in nuclear reactor fuel bundles

    International Nuclear Information System (INIS)

    Lim, E.Y.

    1976-01-01

    A methodology has been developed to determine the fuel pin enrichment distribution that yields the best approximation to a prescribed power distribution in nuclear reactor fuel bundles. The problem is formulated as an optimization problem in which the optimal pin enrichments minimize the sum of squared deviations between the actual and prescribed fuel pin powers. A constant average enrichment constraint is imposed to ensure that a suitable value of reactivity is present in the bundle. When constraints are added that limit the fuel pins to a few enrichment types, one must determine not only the optimal values of the enrichment types but also the optimal distribution of the enrichment types amongst the pins. A matrix of boolean variables is used to describe the assignment of enrichment types to the pins. This nonlinear mixed integer programming problem may be rigorously solved with either exhaustive enumeration or branch and bound methods using a modification of the algorithm from the continuous problem as a suboptimization. Unfortunately these methods are extremely cumbersome and computationally overwhelming. Solutions which require only a moderate computational effort are obtained by assuming that the fuel pin enrichments in this problem are ordered as in the solution to the continuous problem. Under this assumption search schemes using either exhaustive enumeration or branch and bound become computationally attractive. An adaptation of the Hooke--Jeeves pattern search technique is shown to be especially efficient

  13. Hydrogen-enriched fuels

    Energy Technology Data Exchange (ETDEWEB)

    Roser, R. [NRG Technologies, Inc., Reno, NV (United States)

    1998-08-01

    NRG Technologies, Inc. is attempting to develop hardware and infrastructure that will allow mixtures of hydrogen and conventional fuels to become viable alternatives to conventional fuels alone. This commercialization can be successful if the authors are able to achieve exhaust emission levels of less than 0.03 g/kw-hr NOx and CO; and 0.15 g/kw-hr NMHC at full engine power without the use of exhaust catalysts. The major barriers to achieving these goals are that the lean burn regimes required to meet exhaust emissions goals reduce engine output substantially and tend to exhibit higher-than-normal total hydrocarbon emissions. Also, hydrogen addition to conventional fuels increases fuel cost, and reduces both vehicle range and engine output power. Maintaining low emissions during transient driving cycles has not been demonstrated. A three year test plan has been developed to perform the investigations into the issues described above. During this initial year of funding research has progressed in the following areas: (a) a cost effective single-cylinder research platform was constructed; (b) exhaust gas speciation was performed to characterize the nature of hydrocarbon emissions from hydrogen-enriched natural gas fuels; (c) three H{sub 2}/CH{sub 4} fuel compositions were analyzed using spark timing and equivalence ratio sweeping procedures and finally; (d) a full size pick-up truck platform was converted to run on HCNG fuels. The testing performed in year one of the three year plan represents a baseline from which to assess options for overcoming the stated barriers to success.

  14. Medidas directas e indirectas de las funciones ejecutivas en niños con trastorno de espectro autista

    Directory of Open Access Journals (Sweden)

    María Fernanda Pérez Pichardo

    2018-01-01

    Full Text Available INTRODUCCIÓN: los niños con trastorno del espectro autista (TEA tienen dificultades en las funciones ejecutivas (FE pero se han sugerido discrepancias entre las medidas directas e indirectas.   OBJETIVO: conocer si existen diferencias en las funciones ejecutivas mediante medidas directas e indirectas entre niños con y sin TEA.   MÉTODO: se incluyeron 14 varones entre primero y tercero de primaria, 50% con diagnóstico de TEA y 50% con desarrollo neurotípico, seleccionados por emparejamiento en edad y grado escolar. El promedio de edad de la muestra fue de 7.43 (DE 0.98 años. Se aplicaron las pruebas de Evaluación neuropsicológica de funciones ejecutivas en niños (ENFEN y el Inventario de clasificación comportamental de las funciones ejecutivas (BRIEF.   RESULTADOS: se encontraron diferencias significativas en las medidas de las FE indirectas, pero no en las directas, entre los grupos (p < 0.05. Así mismo, las correlaciones entre las medidas directas e indirectas fueron mayores en el grupo con TEA.   CONCLUSIONES: los niños con TEA son evaluados con más dificultades en las medidas indirectas de las funciones ejecutivas, pero obtienen puntuaciones similares a los niños con desarrollo neurotípico cuando son evaluados en condiciones estructuradas empleando medidas directas.

  15. High enrichment to low enrichment core's conversion. Technical securities

    International Nuclear Information System (INIS)

    Abbate, P.; Madariaga, M.R.

    1990-01-01

    This work presents the fulfillment of the technical securities subscribed by INVAP S.E. for the conversion of a high enriched uranium core. The reactor (of 5 thermal Mw), built in the 50's and 60's, is of the 'swimming pool' type, with light water and fuel elements of the curve plates MTR type, enriched at 93.15 %. These are neutronic and thermohydraulic securities. (Author) [es

  16. Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography

    NARCIS (Netherlands)

    Ruprecht, Benjamin; Koch, Heiner; Domasinska, Petra; Frejno, Martin; Kuster, Bernhard; Lemeer, Simone

    2017-01-01

    Phosphorylation is among the most important post-translational modifications of proteins and has numerous regulatory functions across all domains of life. However, phosphorylation is often substoichiometric, requiring selective and sensitive methods to enrich phosphorylated peptides from complex

  17. VIOLENCIA DE GÉNERO EN LAS UNIVERSIDADES: INVESTIGACIONES Y MEDIDAS PARA PREVENIRLA

    Directory of Open Access Journals (Sweden)

    Rosa Larena

    2010-12-01

    Full Text Available Normal 0 21 false false false ES ZH-TW X-NONE MicrosoftInternetExplorer4 En este artículo recogemos los resultados de la investigación “Violencia de género en las universidades españolas”, la primera investigación que analiza la violencia de género en el contexto universitario en España. Las investigaciones sobre esta clase de violencia en el ámbito internacional muestran, por un lado, que es una realidad existente en la universidad, de la misma forma que lo es en otros ámbitos sociales. Por otro lado, el análisis de las actuaciones implementadas por universidades de prestigio muestra la existencia de diferentes medidas para prevenirla y superarla. Estas evidencias, contrastadas con las percepciones de diferentes colectivos de la comunidad universitaria sobre la aplicabilidad de estas medidas en las universidades españolas, son el punto de partida para iniciar el desarrollo de medidas que erradiquen la violencia de género en nuestras universidades. In this article we present the findings from the research “Gender violence in Spanish universities”, the first study that analyses gender violence in the university context in Spain. Studies on gender violence at the international level show, on the one hand, that gender violence is a reality that exists in universities, in the same way that it exists in other social contexts. On the other hand, the analysis of the actions implemented in prestigious universities shows the existence of measures to prevent and overcome it. Contrasting these evidences with the perceptions of different university collectives about the applicability of these measures in Spanish universities is the starting point to develop measures to eradicate gender violence in our universities.

  18. Safety of uranium enrichment plant

    International Nuclear Information System (INIS)

    Yonekawa, Shigeru; Morikami, Yoshio; Morita, Minoru; Takahashi, Tsukasa; Tokuyasu, Takashi.

    1991-01-01

    With respect to safety evaluation of the gas centrifuge enrichment facility, several characteristic problems are described as follows. Criticality safety in the cascade equipments can be obtained to maintain the enrichment of UF 6 below 5 %. External radiation dose equivalent rate of the 30B cylinder is low enough, the shield is not necessary. Penetration ratio of the two-stage HEPA filters for UF 6 aerosol is estimated at 10 -9 . From the experimental investigation, vacuum tightness is not damaged by destruction of gas centrifuge rotor. Carbon steel can be used for uranium enrichment equipments under the condition below 100degC. (author)

  19. Environmental enrichment for aquatic animals.

    Science.gov (United States)

    Corcoran, Mike

    2015-05-01

    Aquatic animals are the most popular pets in the United States based on the number of owned pets. They are popular display animals and are increasingly used in research settings. Enrichment of captive animals is an important element of zoo and laboratory medicine. The importance of enrichment for aquatic animals has been slower in implementation. For a long time, there was debate over whether or not fish were able to experience pain or form long-term memories. As that debate has reduced and the consciousness of more aquatic animals is accepted, the need to discuss enrichment for these animals has increased. Copyright © 2015 Elsevier Inc. All rights reserved.

  20. World enrichment requirements to 2005

    International Nuclear Information System (INIS)

    Anon.

    1991-01-01

    The primary enrichment suppliers-Eurodif, Techsnabexport, Urenco, and the US DOE - are positioning themselves to take advantage of the post - 1995 market. Overall, unfilled requirements represent about 40 percent of world requirements in the year 2000. The USA will be the primary market, as US utilities' unfilled enrichment requirements account for over 60 percent of the world's total unfilled requirements. The enrichment market is moving toward more global competition, as each supplier tries to maintain its current regional market base and then to capture additional market share in other regions

  1. Normatização de medidas acústicas da voz normal

    Directory of Open Access Journals (Sweden)

    Araújo Simone Adad

    2002-01-01

    Full Text Available Introdução: A análise acústica da voz com o avanço da tecnologia digital surge como um exame complementar promissor para aumentar a precisão diagnóstica em laringologia. Objetivo: Normatização das medidas acústicas de freqüência fundamental, perturbação e ruído na voz normal de brasileiros dos sexos masculino e feminino. Forma de estudo: Clínico prospectivo. Material e Método: Pesquisa no período de março a agosto de 1997, no Ambulatório de Otorrinolaringologia do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, com 80 voluntários da cidade de Ribeirão Preto e região, sendo 40 do sexo masculino e 40 do feminino, selecionados por meio de triagem, foram realizadas gravações digitais dos fonemas /a/, /e/ e /i/, originando uma amostra de 240 sinais acústicos submetidos ao Programa de Análise Acústica da Voz, desenvolvido na Escola de Engenharia de São Carlos da Universidade de São Paulo com obtenção das medidas acústicas. Resultados: Foram obtidos valores médios em relação a sexo e fonemas das medidas de: Freqüência fundamental, Jitter (Directional Perturbation Factor, Perturbation Variation Index, Jitter Ratio, Jitter Factor, Period Perturbation Quotient, Shimmer (Directional Perturbation Factor, Amplitude Variation Index, Amplitude Perturbation Quotient, Spectral Noise Level, Harmonic-to-noise ratio, Harmonic-to-noise ratio cepstrum, Normalized Noise Energy e Breathiness ratio. Conclusão: A normatização das medidas acústicas é necessária para conhecer melhor a voz normal. Os valores obtidos são compatíveis em sua grande maioria com à literatura existente.

  2. Medidas de calidad aplicadas a los levantamientos topográficos en Colombia

    OpenAIRE

    Sabogal Lemus, Carlos Augusto

    2016-01-01

    Este trabajo documenta la investigación “Medidas de calidad aplicadas a los levantamientos topográficos en Colombia”; la propuesta metodológica de este documento, tiene como objetivo principal la realización de la evaluación de calidad de un conjunto de datos levantados en campo, para lo cual es necesario revisar los antecedentes de la topografía en Colombia, así como los diferentes sistemas de referencia que gobiernan la cartografía del país -- Previo a realizar una evaluación de calidad, se...

  3. Medidas socioeducativas em meio aberto em Guaíba: entre pressupostos e significados

    OpenAIRE

    Jacques, Luciana Gomes de Lima

    2015-01-01

    No presente estudo, do tipo descritivo e exploratório, de natureza qualitativa, fundamentado no método dialético-crítico, discorre-se sobre os aspectos de proximidade e de distanciamento entre os pressupostos da socioeducação no Sistema Único de Assistência Social (SUAS) e no Sistema Nacional Socioeducativo (SINASE), e analisam-se os significados atribuídos às Medidas Socioeducativas em Meio Aberto (MSE-MA) pelos sujeitos que a protagonizam e/ou que participaram de seu processo de municipaliz...

  4. Medidas de selectividad y sensibilidad en un receptor de AM simplificado

    OpenAIRE

    Przylecki Siwek, Maciej

    2011-01-01

    Se describen las medidas de selectividad y sensibilidad de un receptor de AM simplificado que sólo dispone de una etapa detectora formada por antena de ferrita y un transistor BJT npn polarizado en zona no líneal actuando como un amplificador de clase B. La señal detectada se preamplifica y finalmente se lleva a un amplificador de audio de circuito integrado y al altavoz. Debido a la sencillez la selectividad es variable con la frecuencia de sintonía proporcionando a 392 KHz un...

  5. Pensamiento constructivo como medida de inteligencia emocional en patología dual

    OpenAIRE

    Albalat Alcaraz, Caridad; Fernández Ros, Encarnación; López García, Juan José

    2014-01-01

    Introducción: El objetivo de este estudio es comparar el pensamiento constructivo, una medida de inteligencia emocional, entre una muestra clínica de pacientes adultos diagnosticados de patología dual y una muestra no clínica. Método: Mediante un diseño de investigación comparativo, una muestra no clínica de 835 sujetos se comparó con una muestra de 180 sujetos diagnosticados de patología dual: 78 con trastornos clínicos o del Eje I y102 con trastornos de la personalidad o del ...

  6. Significados de las medidas de tendencia central : un estudio con alumnos universitarios de carreras de humanidades.

    OpenAIRE

    Sayritupac Gutierrez, Javier

    2013-01-01

    En el presente trabajo de investigación se analiza los significados personales e institucionales de las medidas de tendencia central en un estudio con alumnos de primeros ciclos de las carreras de humanidades de la Pontificia Universidad Católica del Perú. Para realizar este análisis se consideró como marco teórico el Enfoque Ontosemiótico de la Cognición e Instrucción Matemática (EOS), el cual nos brindó las herramientas necesarias para el análisis de nuestro objeto de estu...

  7. El tecnoestrés: un estudio del desarrollo de diferentes instrumentos de medida

    OpenAIRE

    Ventura, Mercedes; Llorens Gumbau, Susana; Salanova Soria, Marisa

    2005-01-01

    Onzenes Jornades de Foment de la Investigació de la FCHS (Any 2005-2006) El uso de las Tecnologías de la Información y la Comunicación (TICs) como herramienta de trabajo está teniendo un gran impacto en el mundo laboral. A pesar de que en si misma la tecnología es ‘neutra’ también puede generar consecuencias negativas en los usuarios de TIC quienes pueden sufrir el llamado ‘tecnoestrés’. El concepto y por tanto, la medida de este fenómeno han sufrido diferentes variaciones a lo la...

  8. Medidas de indices de refração de cristais liquidos esmecticos

    OpenAIRE

    Kroin, Teodosio

    1980-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina. Centro de Ciencias Fisicas e Matematicas Mostramos que os cristais líquidos biaxiais podem ter seus índices de refração principais medidos pelo método de Abbe, desde que convenientemente alinhados. Apresentam-se as medidas dos índices de refração das diferentes mesofases e da fase isotrópica do cristal líquido uni-axial 4-butoxibenzilideno-4'- aminoacetofenona em função da temperatura e para três comprimentos de onda. As medi...

  9. Abdominoplastia y pexia de pubis: estandarización de medidas

    OpenAIRE

    O. Ballestas-Campo; A. Pomerane

    2014-01-01

    La abdominoplastia es el procedimiento de elección para corregir la flacidez abdominal, la lipodistrofia supra e infraumbilical y la diástasis de los rectos abdominales. Los nuevos métodos añaden liposucción para definir el contorno corporal. Presentamos una estandarización de medidas para lograr una cicatriz abdominal baja y pexia pubiana en casos de lipodistrofias tipo III y IV de Matarasso. Realizamos un estudio observacional, descriptivo y retrospectivo, evaluando las historias clínicas y...

  10. La educación tributaria como medida para incrementar la recaudación fiscal

    OpenAIRE

    Tapia, Monica; Gaona, Wilmer; Tumbaco, Pedro

    2009-01-01

    La presente tesis está basada en el análisis de la La Educación Tributaria como Medida para Incrementar la Recaudación Fiscal en Ecuador; la importancia de este tema es incrementar la base de contribuyentes, aumentando la Recaudación Fiscal que ayudará a desarrollar planes de inversión y una estabilidad tributaria. Dentro de este estudio es necesario un análisis completo de naturaleza teórica, donde se examinaría la actividad financiera del Estado, la importancia en lo económico, ...

  11. Centar's gas centrifuge enrichment project

    International Nuclear Information System (INIS)

    Abajian, V.V.; Fishman, A.M.

    1976-01-01

    Plans for the building and operating of Centar Associates gas centrifuge uranium enrichment plant are described. Operating costs and machine manufacture are considered. Commitments with the utilities are summarised. (U.K.)

  12. Extraosseus enrichments in bone scintigraphy

    International Nuclear Information System (INIS)

    Jochens, R.; Schumacher, T.; Amthauer, H.; Wolter, M.; Stock, W.; Stroszczynski, C.; Moersler, J.P.; Eichstaedt, H.

    1996-01-01

    Extraosseus enrichments are common findings in bone scintigraphy. Main causes are artifacts by skin or cloth contamination, paravenous and subcutaneous injection. Physical examination, removal of cloths, skin cleaning or further images in differing projections lead to the correct diagnosis artefact or extraosseous enrichments. Further on, extraosseous enrichments are seen in physiological variants. In different diseases extraosseous enrichments are common, especially in urinary tract, liver and extremities. Further diagnostics, e.g. conventional radiologic procedures, sonography and CT scans, have to be performed. In individual cases side results in bone scintigraphy lead to formerly unknown diagnosis, further diagnostic procedure is influenced decisively. Own cases show for example a cerebral apoplectic insult, formerly unknown liver metastasis or metastasis in extraosseous Ewings's sarcoma. (orig.) [de

  13. Uranium enrichment: an evolving market

    International Nuclear Information System (INIS)

    Longenecker, J.; Witzel, R.

    1997-01-01

    With over half of the world uranium enrichment market uncommitted to any supplier early in the next century, competition is certain to be fierce. In the meantime the outlood remains unclear, with the market dominated by a number of developments -privatisation of the United States Enrichment Corp (USEC), increasing availability of Russian and US military inventories, the deployment of advanced technology and the closure of nuclear power plants due to deregulation. (author)

  14. Unjust enrichment in business law

    OpenAIRE

    Vydrová, Zuzana

    2016-01-01

    This thesis analyses the concept of unjust enrichment under the business law. First of all the thesis explains the term of business law. Business law is a complex of legal rules concerning the contractual relationships between entrepreneurs arising from their business activities. Business law is a comprehensive field of law which extends into many other fields of law, both private and public law. Equally the regulation of unjust enrichment within the business law expands into many other laws ...

  15. Colinesterasas en sangre total medidas con técnica semicuantitativa y en eritrocitos o plasma medidas con técnicas cuantitativas: relaciones

    Directory of Open Access Journals (Sweden)

    Jaime Carmona-Fonseca

    2007-06-01

    Resultados. Se evaluaron 827 trabajadores. Según la técnica Lovibond® se clasificaron en cuatro categorías: 821 con valores de 75% o más de actividad colinesterásica (categorías 75%, 87,5% y 100% y otros seis con actividad inferior a 75%. Con cada técnica cuantitativa los valores promedios de colinesterasa eritrocitaria y plasmática correspondientes a los cuatro valores de Lovibond® fueron estadísticamente diferentes entre sí. Los promedios de cada técnica cuantitativa fueron mayores a medida que creció el valor de la técnica semicuantitativa. Con Lovibond® se clasificó muy mal (61%-73% la baja actividad enzimática eritrocitaria, pero la clasificación de la baja actividad plasmática fue absolutamente errónea (94%-96%. Conclusión. Es posible estimar en forma adecuada los valores de colinesterasa eritrocitaria o plasmática que corresponden a las técnicas de Michel y EQM® a partir de datos de la técnica Lovibond® y viceversa, pero sólo cuando la actividad colinesterásica es normal, pues Lovibond® tiene una capacidad muy deficiente para identificar como "bajos" los valores que realmente lo son según las pruebas cuantitativas.

  16. Tasa de reincidencia de la delincuencia juvenil de Extremadura. Medidas privativas de libertad Tasa de reincidencia de la delincuencia juvenil de Extremadura. Medidas privativas de libertad

    Directory of Open Access Journals (Sweden)

    Felipe Núñez Izquierdo

    2012-04-01

    Full Text Available Después de más de 10 años de entrada en vigor de la Ley Orgánica 5/2000, de 12 de enero, de Responsabilidad Penal del Menor, se hace imprescindible realizar un estudio sobre la reincidencia juvenil en Extremadura, para conocer el impacto de dicho precepto. No cabe duda que uno de los problemas que más alarma social genera en la población es la delincuencia cometida por los menores con responsabilidad penal. Inicialmente se hace un repaso a la literatura criminológica actual sobre la reincidencia, programas de prevención, tratamiento a menores delincuentes y la evaluación de la eficacia de dichos programas. En el presente trabajo, siguiendo la metodología de otros estudios nacionales como el de Catalunya, analizamos la reincidencia de la delincuencia juvenil en Extremadura, concretamente de las medidas privativas de libertad. Se analizan los expedientes judiciales cerrados de aquellos jóvenes que hubieran finalizado una medida de este tipo en el año 2007 y se realiza el seguimiento de los mismos hasta el año 2010. En el año 2007, 115 jóvenes cumplieron o estaban cumpliendo una medida privativa de libertad en un Centro de Menores o Permanencia Fin de Semana, de los cuales 63 (57 varones y 7 mujeres resultaron pertenecer a la muestra final objeto de análisis. Los resultados obtenidos revelan que la tasa de reincidencia es del 52,4%, similar a la de otras Comunidades Autónomas, como País Vasco o Catalunya, e inferior a la de Asturias. Otro dato relevante es que un 41,9% de los menores objeto de estudio presentaban antecedentes, por lo que se refleja un comienzo en carreras delictivas acorde con los estudios existentes. La presente investigación ayuda a conocer la eficacia de los diferentes programas que se aplican a los menores delincuentes que cumplen medidas de internamiento, así como también hace una crítica de ésta al situarla con una elevada tasa de reincidencia.After more than 10 years of the entry into force of the

  17. Research on aerodynamic means of isotope enrichment

    International Nuclear Information System (INIS)

    Cattolica, R.J.; Gallagher, R.J.; Talbot, L.; Willis, D.R.; Hurlbut, F.C.; Fiszdon, W.; Anderson, J.B.

    1978-03-01

    The results of a research program directed toward the understanding of the fundamental gas dynamics involved in aerodynamic isotope enrichment are summarized. The specific aerodynamic isotope enrichment method which was examined in this research is based on a velocity slip phenomenon which occurs in the rarefied hypersonic expansion of a heavy molecular weight gas and a light carrier gas in a nozzle or free jet. This particular aerodynamic method was chosen for study because it contains the fundamental molecular physics of other more complex techniques within the context of a one-dimensional flow without boundary effects. From both an experimental and theoretical modeling perspective this provides an excellent basis for testing the experimental and numerical tools with which to investigate more complex aerodynamic isotope enrichment processes. This report consists of three separate parts. Part I contains a theoretical analysis of the velocity slip effect in free jet expansions of binary and ternary gas mixtures. The analysis, based on a source flow model and using moment equations is derived from the Boltzmann equation using the hypersonic approximation. Part II contains the experimental measurements of velocity slip. The numerical simulation of the slip process was carried out by using a Monte-Carlo numerical technique. In addition, comparisons between the theoretical analysis of Part I and the experiments are presented. Part III describes impact pressure measurements of free jet expansions from slot shaped two dimensional nozzles. At least two methods of aerodynamic isotope enrichment (opposed jet and velocity slip) would depend on the use of this type of two dimensional expansion. Flow surveys of single free jet and the interferene of crossed free jets are presented

  18. A revelação de abuso sexual: as medidas adotadas pela rede de apoio

    Directory of Open Access Journals (Sweden)

    Luísa Fernanda Habigzang

    Full Text Available O presente estudo identificou e analisou as medidas adotadas pela rede de apoio de crianças e adolescentes após a revelação de abuso sexual. Participaram 40 meninas, entre oito e 16 anos, vítimas de abuso sexual. As medidas de proteção adotadas pela rede foram mapeadas através de entrevista semi-estruturada. A revelação foi feita aos pais em 42,5% da amostra e 92,5% das pessoas acreditaram. O abrigamento ocorreu em 35% dos casos e o restante permaneceu com a família que afastou o agressor. A atitude de confiança da família na revelação e a denúncia da violência constituíram-se em um fator de proteção. Contudo, o alto índice de abrigamento e o não acompanhamento efetivo do afastamento do agressor representaram fatores de risco.

  19. La variable latente calidad medida a través del modelo de Rasch.

    Directory of Open Access Journals (Sweden)

    Guerrero, Mª M.

    1997-01-01

    Full Text Available La obtención de aceites de oliva de calidad requiere unos cuidados especiales en todas y cada una de las sucesivas fases de elaboración de los mismos, es una cadena que comienza en el olivo y termina en el consumo. Condición indispensable para ello es conocer las características organolépticas del aceite (a fin de potenciar sus atributos y eliminar, en la medida de los posible, sus defectos, y la influencia del proceso de elaboración en las mismas. El control y medida de estos caracteres se hará mediante las catas, realizadas por expertos catadores utilizando para ello las fichas de cata del Consejo Oleícola Internacional. El modelo de Rasch, que emplearemos para obtener la evaluación sensorial de los aceites de oliva virgen, nos permitirá no sólo dar una calificación organoléptica global al aceite (que ya podemos realizar sin necesidad de aplicar ningún método, siguiendo la metodología de valoración del Consejo Oleícola Internacional, sino también clasificar y medir los factores sensoriales que intervienen en la calidad, lo que nos permitirá realizar las acciones oportunas encaminadas a obtener aceites de oliva de cualidades óptimas.

  20. Fast Enrichment Screening for Safeguards Applications

    International Nuclear Information System (INIS)

    Simpson, A.; McElhaney, S.

    2010-01-01

    Methods for rapid non-destructive uranium enrichment classification of large containers are of importance to safeguards and counter-terrorism agencies. There is a need to quickly categorize and segregate suspect items as 'depleted' or 'enriched' on a 'Go/No Go' basis. Recent improvements in gamma spectroscopy technologies have provided the capability to perform rapid field analysis using portable and hand-held devices such as battery-operated medium and high resolution detectors (including lanthanum halide and high purity germanium). Furthermore a new generation of portal monitors are currently under development with advanced spectroscopic capabilities. Instruments and technologies that were previously the domain of complex lab systems are now widely available as touch-screen 'off-the-shelf' units. Despite such advances, the task of enrichment analysis remains a complex exercise. This is particularly so when surveying large items such as drums and crates containing debris of unknown density and composition contaminated with uranium. The challenge is equally applicable to safeguards inspectors evaluating large items and for interdiction of illicit special nuclear materials in mass transport e.g. shipping containers at ports and borders. The variable shielding, container size, lack of matrix knowledge, wall thickness and self-shielding compound this problem. Performing an accurate assessment within the short count time window demanded of the field operative, leads to the need for a reliable method that can adapt to such conditions and is robust to a wide dynamic range of counting statistics. Several methods are evaluated with reference to the performance metrics defined in applicable standards. The primary issue is to minimize the bias that can result from attenuation effects, particularly as the gamma emissions from U235 are low energy and therefore highly susceptible to absorption in large containers with metal scrap. Use of other radiometric signatures such as

  1. NAA analysis of enriched Zn-68 by

    International Nuclear Information System (INIS)

    Rafii, H.; Mirzaei, M.; Mirzajani, N.; Sardari, D.; Shahabi, I.; Majedi, F.

    2002-01-01

    Excessive application of enriched isotopes in various fields of sciences and industry necessitates measuring their abundant by a precise and rapid methos. Beside the inductively coupled plasma mass spectrometry, the thermal neutron activation analysis, NAA, is an alternative method, which is capable to determine trace amounts of elements as well as the elemental abundance. In this article the enrichment of Zn-68 in two different samples has been studied by mean of NAA. One sample was separated by an electromagnetic system in our center and the other was purchased from a French company, Cortecnet. The neutron irradiation was took place in MNSR reactor by flux 10 1 1n/cm 2 sec. for 30 min. and the produced radioactivity from Zn-69 m was measured one day after irradiation by HPGe detector. The results shows a good agreement with the reported ones and its low derivation of about ±3.05 indicates that the NAA is a precise, rapid, and supplemental method for analyzing enriched Zn-68

  2. Advanced uranium enrichment technologies

    International Nuclear Information System (INIS)

    Merriman, R.

    1983-01-01

    The Advanced Gas Centrifuge and Atomic Vapor Laser Isotope Separation methods are described. The status and potential of the technologies are summarized, the programs outlined, and the economic incentives are noted. How the advanced technologies, once demonstrated, might be deployed so that SWV costs in the 1990s can be significantly reduced is described

  3. The gas centrifuge, uranium enrichment and nuclear proliferation

    International Nuclear Information System (INIS)

    Chapman, A.

    1988-01-01

    The author considers the consequences for controlling nuclear proliferation of the emergence of the gas centrifuge method for enriching uranium and succeeds in the difficult and delicate task of saying enough about gas centrifuge techniques for readers to judge, what may be involved in fully embracing gas centrifuge enrichment within the constraints of an anti-proliferation strategy, whilst at the same time saying nothing that could be construed as encouraging an interest in the gas centrifuge route to highly enriched uranium where none had before existed. (author)

  4. OPTIMASI PEMADATAM CEPAT PADA PENGAYAAN MINYAK IKAN HASIL SAMPING PENGALENGAN LEMURU DENGAN ASAM LEMAK w-3 MENGGUNAKAN METODE PERMUKAAN RESPON [Optimization of Rapid Solidification to Enrich Fish Oil from by-Product of Lemuru Canning Processing with w-3 batty Acids by Response Surface Method

    Directory of Open Access Journals (Sweden)

    Teti Estiasih1

    2005-12-01

    Full Text Available Oil from by-product of lemuru canning processing was a source of w-3 fatty acid but its characteristics had out been known. The content of w-3 fatty acids of this oil had to be increased. Various methods are available to enhance w-3 fatty acids concentration Rapid solidification was one of limited methods to enrich fish oil by w-3 fatty acids containing triglycerides.This research was conducted to optimize rapid solidification condition to enrich fish oil from by product of lemuru canning processing with w-3 fatty acids and characterize the enriched oil compared by International Association of Fish Meal and Oil Manufacturers standard. In optimization process, the content of EPA+OHA and yield .was maximized. A two-factors central composite design in Response Surface Method was used to study the effect of solvent-to-oil ratio (X1 and extraction time (X2. The response (Y is the multiplication of EPA+DHA content by yield.The results showed that under optimum conditions the maximum response were obtained at a solvent-to-oil ratio of 3,95:1(vw and extraction time of 24,93 hours. The w-3 fatty acids enriched fish oil had EPA+DHA content of 33,33% and yield of 9.40% (w/w.The produced w-3 fatty acids enriched fish oil had good quality based on food grade fish oil standard, unless Fe and Cu content. Chelation could reduce these oxidizing metals.

  5. PWR fuel of high enrichment with erbia and enriched gadolinia

    International Nuclear Information System (INIS)

    Bejmer, Klaes-Håkan; Malm, Christian

    2011-01-01

    Today standard PWR fuel is licensed for operation up to 65-70 MWd/kgU, which in most cases corresponds to an enrichment of more than 5 w/o "2"3"5U. Due to criticality safety reason of storage and transportation, only fuel up to 5 w/o "2"3"5U enrichment is so far used. New fuel storage installations and transportation casks are necessary investments before the reactivity level of the fresh fuel can be significantly increased. These investments and corresponding licensing work takes time, and in the meantime a solution that requires burnable poisons in all pellets of the fresh high-enriched fuel might be used. By using very small amounts of burnable absorber in every pellet the initial reactivity can be reduced to today's levels. This study presents core calculations with fuel assemblies enriched to almost 6 w/o "2"3"5U mixed with a small amount of erbia. Some of the assemblies also contain gadolinia. The results are compared to a reference case containing assemblies with 4.95 w/o "2"3"5U without erbia, utilizing only gadolinia as burnable poison. The comparison shows that the number of fresh fuel assemblies can be reduced by 21% (which increases the batch burnup by 24%) by utilizing the erbia fuel concept. However, increased cost of uranium due to higher enrichment is not fully compensated for by the cost gain due to the reduction of the number assemblies. Hence, the fuel cycle cost becomes slightly higher for the high enrichment erbia case than for the reference case. (author)

  6. Monitoreo neurointensivo en pediatría (III: Tratamiento. Medidas generales

    Directory of Open Access Journals (Sweden)

    Eduardo M. Pleguezuelo Rodríguez

    2001-06-01

    Full Text Available Se informa que con el neuromonitoreo y el manejo intensivo del traumatismo craneoencefálico grave, se han identificado un grupo de fenómenos fisiopatológicos diferentes, los cuales requieren de medidas disímiles y específicas para cada paciente, lo que hace más racional el tratamiento de ellos. De la misma manera esto ha propiciado que cambien radicalmente las concepciones acerca de antiguos patrones terapéuticos establecidos durante muchos años; ejemplo de esto es el cambio en la reposición hídrica de ellos, los cuales antes se mantenían sobre una vertiente seca y hoy se preconiza la euvolemia estricta, la sustitución de la hiperventilación de rutina por la ventilación optimizada, cambios en la política del uso de agentes osmóticos, la terapia barbitúrica y la craneotomía descompresiva en casos de hipertensión intracraneal refractaria a medidas terapéuticas convencionales. En este trabajo se hace una revisión actualizada de las principales medidas terapéuticas utilizadas en estos pacientes, acorde con lo que plantean los lineamientos para el tratamiento del traumatismo craneal grave de la Asociación Americana de Cirujanos Neurológicos y los del consorcio europeo para la lesión cerebral. Las medidas terapéuticas utilizadas en estos pacientes se dividen en 2 fases. En este tercer trabajo de actualización se aborda el manejo de la primera fase que corresponde a la estabilización urgente inicial antes de establecer la medición de la presión intracraneal (PIC, así como las medidas generales de la segunda fase una vez iniciado el monitoreo de la PIC. Principios terapéuticos que desde hace algunos años se vienen utilizando en el Hospital provincial General y el Hospital Pediátrico Provincial Docente de Camagüey para el manejo del trauma craneal grave, los cuales se basan en medidas iniciales en la recepción, acorde con lo que plantean los principios del soporte vital avanzado en trauma para la reanimaci

  7. Una medida de eficiencia de mercado. Un enfoque de teoría de la información

    Directory of Open Access Journals (Sweden)

    Reyna Susana García Ruiz

    2014-01-01

    Full Text Available En este trabajo, con base en el concepto de entropía de Shan-non, se propone una medida de eficiencia de mercado que uti-liza la función de densidad empírica de los rendimientos comoalfabeto para calcular la entropía del sistema y proveer con ellouna medida de eficiencia de mercado. Se demuestra que bajociertas condiciones de ergodicidad y de estacionariedad la en-tropía muestral converge a la entropía del estado dominante,lo que valida el uso de la entropía muestral como medida deeficiencia del sistema. Asimismo, se demuestra que la medidapropuesta es consistente con algunos de los axiomas de Artz-neret al.(1999 acerca de una medida coherente de riesgo. Porúltimo, con fines ilustrativos, se llevan a cabo varias aplicacio-nes de la medida de eficiencia propuesta a distintos mercadosde capitales: DJIA, S&P500, FTSE100 e IPC.

  8. High enrichment to low enrichment core's conversion. Accidents analysis

    International Nuclear Information System (INIS)

    Abbate, P.; Rubio, R.; Doval, A.; Lovotti, O.

    1990-01-01

    This work analyzes the different accidents that may occur in the reactor's facility after the 20% high-enriched uranium core's conversion. The reactor (of 5 thermal Mw), built in the 50's and 60's, is of the 'swimming pool' type, with light water and fuel elements of the curve plates MTR type, enriched at 93.15 %. This analysis includes: a) accidents by reactivity insertion; b) accidents by coolant loss; c) analysis by flow loss and d) fission products release. (Author) [es

  9. Gaseous diffusion -- the enrichment workhorse

    International Nuclear Information System (INIS)

    Shoemaker, J.E. Jr.

    1984-01-01

    Construction of the first large-scale gaseous diffusion facility was started as part of the Manhattan Project in Oak Ridge, Tennessee, in 1943. This facility, code named ''K-25,'' began operation in January 1945 and was fully on stream by September 1945. Four additional process buildings were later added in Oak Ridge as the demand for enriched uranium escalated. New gaseous diffusion plants were constructed at Paducah, Kentucky, and Portsmouth, Ohio, during this period. The three gaseous diffusion plants were the ''workhorses'' which provided the entire enriched uranium demand for the United States during the 1950s and 1960s. As the demand for enriched uranium for military purposes decreased during the early 1960s, power to the diffusion plants was curtailed to reduce production. During the 1960s, as plans for the nuclear power industry were formulated, the role of the diffusion plants gradually changed from providing highly-enriched uranium for the military to providing low-enriched uranium for power reactors

  10. Uranium enrichment. Technology, economics, capacity

    International Nuclear Information System (INIS)

    Voigt, W.R. Jr.; Saire, D.E.; Gestson, D.K.; Peske, S.E.; Vanstrum, P.R.

    1983-01-01

    Large-scale enrichment of uranium has now been carried out for 40 years. While the gaseous diffusion process was the original choice of several countries and continues today to provide the major component of the world production of separative work, the last two decades have witnessed the development of a number of alternative processes for enrichment. These processes, which are being studied and deployed around the world, offer a wide range of technical and economic characteristics which will be useful in assuring adequate capacity to meet projected reactor fuel market needs through the rest of this century at competitive prices. With present uncertainties in future enriched uranium needs, it is apparent that flexibility in the deployment and operation of any enrichment process will be one of the prime considerations for the future. More economical production of separative work not only can have a beneficial impact on reactor fuel costs, but also tends to conserve natural uranium resources. This paper reviews the world scene in the enrichment component of the fuel cycle, including existing or planned commercial-scale facilities and announced R+D efforts on various processes. (author)

  11. Uranium enrichment: technology, economics, capacity

    Energy Technology Data Exchange (ETDEWEB)

    Voigt, Jr., W. R.; Vanstrum, P. R.; Saire, D. E.; Gestson, D. K.; Peske, S. E.

    1982-08-01

    Large-scale enrichment of uranium has now been carried out for 40 years. While the gaseous diffusion process was the original choice of several countries and continues today to provide the major component of the world production of separative work, the last two decades have witnessed the development of a number of alternative processes for enrichment. These processes, which are being studied and deployed around the world, offer a wide range of technical and economic characteristics which will be useful in assuring adequate capacity to meet projected reactor fuel market needs through the rest of this century at competitive prices. With present uncertainties in future enriched uranium needs, it is apparent that flexibility in the deployment and operation of any enrichment process will be one of the prime considerations for the future. More economical production of separative work not only can have a beneficial impact on reactor fuel costs, but also tends to conserve natural uranium resources. This paper reviews the world scene in the enrichment component of the fuel cycle, including existing or planned commercial-scale facilities and announced R and D efforts on various processes.

  12. Uranium enrichment: technology, economics, capacity

    International Nuclear Information System (INIS)

    Voigt, W.R. Jr.; Vanstrum, P.R.; Saire, D.E.; Gestson, D.K.; Peske, S.E.

    1982-01-01

    Large-scale enrichment of uranium has now been carried out for 40 years. While the gaseous diffusion process was the original choice of several countries and continues today to provide the major component of the world production of separative work, the last two decades have witnessed the development of a number of alternative processes for enrichment. These processes, which are being studied and deployed around the world, offer a wide range of technical and economic characteristics which will be useful in assuring adequate capacity to meet projected reactor fuel market needs through the rest of this century at competitive prices. With present uncertainties in future enriched uranium needs, it is apparent that flexibility in the deployment and operation of any enrichment process will be one of the prime considerations for the future. More economical production of separative work not only can have a beneficial impact on reactor fuel costs, but also tends to conserve natural uranium resources. This paper reviews the world scene in the enrichment component of the fuel cycle, including existing or planned commercial-scale facilities and announced R and D efforts on various processes

  13. Abdominoplastia y pexia de pubis: estandarización de medidas

    Directory of Open Access Journals (Sweden)

    O. Ballestas-Campo

    2014-06-01

    Full Text Available La abdominoplastia es el procedimiento de elección para corregir la flacidez abdominal, la lipodistrofia supra e infraumbilical y la diástasis de los rectos abdominales. Los nuevos métodos añaden liposucción para definir el contorno corporal. Presentamos una estandarización de medidas para lograr una cicatriz abdominal baja y pexia pubiana en casos de lipodistrofias tipo III y IV de Matarasso. Realizamos un estudio observacional, descriptivo y retrospectivo, evaluando las historias clínicas y las fotografías sobre 420 mujeres, con edades entre 18 y 63 años, intervenidas entre 2004 y 2011. Basamos la técnica quirúrgica en los siguientes principios: referencias anatómicas; medidas estandarizadas con la paciente de pie sobre zapatos de tacón alto para el trazado de una incisión suprapúbica baja, siguiendo diseño de los autores; pexia de pubis y región inguinal; cicatriz suprapúbica baja cubierta por un biquini estándar. El 70,95 % de los casos (298 pacientes correspondieron a abdomen grado IV de lipodistrofia, el 28,57 % (120 pacientes al grado III y el 0,47 % (2 pacientes al grado II según Matarasso. Las complicaciones más frecuentes fueron: seroma, (4,04 % , alteraciones cicatríciales (7,37 % , necrosis parcial y epidermolisis (2,38 % y estenosis umbilical (1,19 % . Como conclusión creemos que la estandarización de medidas para el diseño de la cicatriz en la abdominoplastia permite obtener resultados estéticos predecibles y funcionales buenos: cicatriz baja cubierta por un biquini o ropa interior estándar, cicatriz umbilical ovalada, pexia de pubis y excelente definición del contorno abdominal y corporal, con baja tasa de complicaciones.

  14. The economic emergency: an insufficient solution La emergencia económica: una medida inconclusa

    Directory of Open Access Journals (Sweden)

    Lorente Luis

    1997-06-01

    Full Text Available There are many reasons for the Colombian recession that began in 1995, but the disagreement between fiscal and monetary authorities is an outstanding one. Monetary measures against inflation promoted high interest rates, made real investment more risky, rocketed the exchange rate, attracted foreign capital and elevated public debt costs.
    Emergency measures announced early in the year were insufficient because the problem comes from old and needs long range measures. Balanced budget is a valid target, but along the economic cycle and not in each fiscal year. Reduction of public expenses or public debt must be applied in periods of growth and not when taxes go down because a recession has begun. Monetary policy must allow for anti-cyclical measures and can do it effectively by controlling the volume. of credit accessible to the Government, or to finance private construction of public works, or to those that will buy the facilities and services that get privatized.La fase recesiva de la economía colombiana que comenzó en 1995 tiene varias causas, en especial, el desacuerdo entre políticas fiscales y monetarias. Las medidas antiinflacionarias elevaron las tasas de interés y dificultaron la inversión, y provocaron la revaluación de la moneda, la promoción de la entrada masiva de capital externo y del encarecimiento del servicio de la deuda pública. Las medidas de emergencia anunciadas a comienzos de 1995 no fueron suficientes: se necesita una política con objetivos de largo plazo. Hay que buscar un balance fiscal a lo largo del ciclo económico y no en cada año fiscal; la contracción del gasto público debe aplicarse en las fases de auge económico y no cuando caen los recaudos porque ya comenzó una recesión. La política monetaria debe permitir medidas anticíclicas y puede contribuir a ellas si regula el volumen de crédito accesible al Estado, a sus contratistas y a quienes adquieren prvatizaciones.

  15. SERVIÇO SOCIAL, MEDIDAS SOCIOEDUCATIVAS E O ADOLESCENTE INFRATOR NO LIBERDADE ASSISTIDA

    Directory of Open Access Journals (Sweden)

    Cristiano Costa Carvalho

    2013-10-01

    Full Text Available RESUMO: A temática dos adolescentes em conflito com a Lei é uma questão que merece uma atenção especial, pois é significativa ao longo da trajetória pela conquista da mudança de paradigma na legislação em relação ao enfrentamento desta expressão da questão social, e porque mesmo diante desta mudança, as medidas em meio aberto são as que têm sido menos aplicadas. Deste modo, esta pesquisa permitiu conhecer e problematizar a atuação do profissional de Serviço Social dentro da política de atendimento aos adolescentes em conflito com a Lei e em destaque a medida socioeducativa no programa de Liberdade Assistida, devido a sua característica educativa que preserva o convívio social, familiar e comunitária.  O processo de preparação desta pesquisa consistiu em entrevistas semi-estruturadas, estudos bibliográficos, documentais, e acervos digitais. Foi analisado o potencial da atuação profissional em consonância com as diretrizes previstas pelo Estatuto da Criança e do Adolescente, Lei que garante aos mesmos um atendimento especial, e reconhece que são sujeitos em processo de formação. PALAVRAS-CHAVE: adolescentes, ato infracional, medidas socioeducativas ABSTRACT: The topic of minors in conflict with the Law is an issue that deserves special attention due to its representativeness in the course of attaining a paradigm shift in the legislation related to this social matter, and because even before this change takes place, it is the open regime measures that have been less applied. This research has therefore helped identify and discuss the role of the Social Worker in the assistance policy for juveniles in conflict with the Law, highlighting the socio-educational measure of the probation program “Liberdade Assistida”, which, due to its educational nature, preserves the social, family and community life. The process of preparing this research consisted of semi-structured interviews, bibliographical and documentary

  16. The Passive Neutron Enrichment Meter for Uranium Cylinder Assay

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Karen A.; Menlove, Howard O.; Swinhoe, Martyn T.; Marlow, Johanna B. [Safeguards Science and Technology Group (N-1), Los Alamos National Laboratory, Los Alamos (United States)

    2011-12-15

    As fuel cycle technology becomes more prevalent around the world, international safeguards have become increasingly important in verifying that nuclear materials have not been diverted. Uranium enrichment technology is a critical pathway to nuclear weapons development, making safeguards of enrichment facilities especially important. Independently-verifiable material accountancy is a fundamental measure in detecting diversion of nuclear materials. This paper is about a new instrument for uranium cylinder assay for enrichment plant safeguards called the Passive Neutron Enrichment Meter (PNEM). The measurement objective is to simultaneously verify uranium mass and enrichment in Uf6 cylinders. It can be used with feed, product, and tails cylinders. Here, we consider the enrichment range up to 5% {sup 235}U. The concept is to use the Doubles-to-Singles count rate to give a measure of the {sup 235}U enrichment and the Singles count rate to provide a measure of the total uranium mass. The cadmium ratio is an additional signature for the enrichment that is especially useful for feed and tails cylinders. PNEM is a {sup 3}He-based system that consists of two portable detector pods. Uranium enrichment in UF{sub 6} cylinders is typically determined using a gamma-ray-based method that only samples a tiny volume of the cylinder's content and requires knowledge of the cylinder wall thickness. The PNEM approach has several advantages over gamma-ray-based methods including a deeper penetration depth into the cylinder, meaning it can be used with heterogeneous isotopic mixtures of UF{sub 6}. In this paper, we describe a Monte Carlo modelling study where we have examined the sensitivity of the system to systematic uncertainties such as the distribution of UF{sub 6} within the cylinder. We also compare characterization measurements of the PNEM prototype to the expected measurements calculated with Monte Carlo simulations.

  17. Enrichment Experiences in Engineering (E[superscript 3]) for Teachers Summer Research Program: An Examination of Mixed-Method Evaluation Findings on High School Teacher Implementation of Engineering Content in High School STEM Classrooms

    Science.gov (United States)

    Page, Cheryl A.; Lewis, Chance W.; Autenrieth, Robin L.; Butler-Purry, Karen L.

    2013-01-01

    Ongoing efforts across the U.S. to encourage K-12 students to consider science, technology, engineering and mathematics (STEM) careers have been motivated by concerns that the STEM pipeline is shrinking because of declining student enrollment and increasing rates of retirement in industry. The Enrichment Experiences in Engineering (E[superscript…

  18. The ENRICH study to evaluate the effectiveness of a combination intervention package to improve isoniazid preventive therapy initiation, adherence and completion among people living with HIV in Ethiopia: Rationale and design of a mixed methods cluster randomized trial

    Directory of Open Access Journals (Sweden)

    Andrea A. Howard

    2017-06-01

    Discussion: The ENRICH Study evaluates a CIP targeting barriers to IPT implementation. If the CIP is found effective and acceptable, this study has the potential to inform TB prevention strategies for HIV patients in resource-limited countries in sub-Saharan Africa.

  19. A Systematic Approach to Marital Enrichment.

    Science.gov (United States)

    Dinkmeyer, Don; Carlson, Jon

    1986-01-01

    Presents a systematic approach to enriching marital relationships. The history and current status of marital enrichment is reviewed. An Adlerian approach to marital enrichment is described. Applications of the program in enrichment groups, marriage therapy and couple groups are included. (Author)

  20. Medida de la evapotranspiración real en coberturas vegetales semiáridas (Cuenca de Mula, Murcia), según las varicaciones de humedad del suelo medidas mediante el procedimiento (TDR)

    OpenAIRE

    Belmonte Serrato, Francisco; Romero Díaz, María Asunción

    2006-01-01

    En este trabajo se presenta un método de medida directa de la Evapotranspiración Real (ETR) bajo distintas coberturas vegetales, basado en las diferencias de humedad en el suelo medidas mediante el procedimiento TDR, en un intervalo temporal de 15 días y su comparación con los valores calculados mediante el método de Thornthwaite, que usa la temperatura media como parámetro fundamental. Los resultados demuestran la validez del método utilizado, habiendo obtenido valores de E...

  1. Equipo adiabático para medida del coeficiente de absorción específico de un material sometido a un campo magnético alterno y método para realizar dicha medida

    OpenAIRE

    Mediano, Arturo; Natividad, Eva; Palacio, Fernando; Castro, Miguel

    2007-01-01

    Equipo adiabático para medida del coeficiente de absorción específico de un material sometido a un campo magnético alterno y método para realizar dicha medida. En esta patente se describe un equipo que permite medir de forma adiabática el incremento de temperatura experimentado por aquellos materiales que presentan mecanismos activos de disipación de calor al someterlos a un campo magnético alterno. Conocida esta magnitud y el tiempo de aplicación del campo y, conocien...

  2. Enrichment of light hydrocarbon mixture

    Science.gov (United States)

    Yang,; Dali, [Los Alamos, NM; Devlin, David [Santa Fe, NM; Barbero, Robert S [Santa Cruz, NM; Carrera, Martin E [Naperville, IL; Colling, Craig W [Warrenville, IL

    2010-08-10

    Light hydrocarbon enrichment is accomplished using a vertically oriented distillation column having a plurality of vertically oriented, nonselective micro/mesoporous hollow fibers. Vapor having, for example, both propylene and propane is sent upward through the distillation column in between the hollow fibers. Vapor exits neat the top of the column and is condensed to form a liquid phase that is directed back downward through the lumen of the hollow fibers. As vapor continues to ascend and liquid continues to countercurrently descend, the liquid at the bottom of the column becomes enriched in a higher boiling point, light hydrocarbon (propane, for example) and the vapor at the top becomes enriched in a lower boiling point light hydrocarbon (propylene, for example). The hollow fiber becomes wetted with liquid during the process.

  3. PC based uranium enrichment analyser

    International Nuclear Information System (INIS)

    Madan, V.K.; Gopalakrishana, K.R.; Bairi, B.R.

    1991-01-01

    It is important to measure enrichment of unirradiated nuclear fuel elements during production as a quality control measure. An IBM PC based system has recently been tested for enrichment measurements for Nuclear Fuel Complex (NFC), Hyderabad. As required by NFC, the system has ease of calibration. It is easy to switch the system from measuring enrichment of fuel elements to pellets and also automatically store the data and the results. The system uses an IBM PC plug in card to acquire data. The card incorporates programmable interval timers (8253-5). The counter/timer devices are executed by I/O mapped I/O's. A novel algorithm has been incorporated to make the system more reliable. The application software has been written in BASIC. (author). 9 refs., 1 fig

  4. Advances in uranium enrichment processes

    International Nuclear Information System (INIS)

    Rae, H.K.; Melvin, J.G.; Slater, J.B.

    1986-05-01

    Advances in gas centrifuges and development of the atomic vapour laser isotope separation process promise substantial reductions in the cost of enriched uranium. The resulting reduction in LWR fuel costs could seriously erode the economic advantage of CANDU, and in combination with LWR design improvements, shortened construction times and increased operational reliability could allow the LWR to overtake CANDU. CANDU's traditional advantages of neutron economy and high reliability may no longer be sufficient - this is the challenge. The responses include: combining neutron economy and dollar economy by optimizing CANDU for slightly enriched uranium fuel; developing cost-reducing improvements in design, manufacture and construction; and reducing the cost of heavy water. Technology is a renewable resource which must be continually applied to a product for it to remain competitive in the decades to come. Such innovation is a prerequisite to Canada increasing her share of the international market for nuclear power stations. The higher burn-up achievable with enriched fuel in CANDU can reduce the fuel cycle costs by 20 to 40 percent for a likely range of costs for yellowcake and separative work. Alternatively, some of the benefits of a higher fissile content can take the form of a cheaper reactor core containing fewer fuel channels and less heavy water, and needing only a single fuelling machine. An opportunity that is linked to this need to introduce an enriched uranium fuel cycle into CANDU is to build an enrichment business in Canada. This could offer greater value added to our uranium exports, security of supply for enriched CANDUs, technological growth in Canada and new employment opportunities. AECL has a study in progress to define this opportunity

  5. La Corte Constitucional colombiana ante las medidas de seguridad y defensa nacional del gobierno de Uribe

    Directory of Open Access Journals (Sweden)

    Miguel Revenga-Sánchez

    2010-03-01

    Full Text Available En este trabajo se describen las medidas de seguridad y defensa nacional impulsadas por el gobierno de Álvaro Uribe durante la vigencia del Estado de conmoción interior, así como el posicionamiento que la Corte Constitucional ha adoptado sobre estas, en ejercicio de su función de control de constitucionalidad. La negativa reiterada de la Alta Corte a dar su visto bueno a determinados preceptos restrictivos de los derechos fundamentales ha provocado que el Ejecutivo colombiano imponga el estatuto antiterrorista como parte del programa de seguridad democrática que lidera, mediante una reforma constitucional que es analizada en el presente artículo.

  6. IMPACTOS DE LAS CARRETERAS SOBRE LA FAUNA SILVESTRE Y SUS PRINCIPALES MEDIDAS DE MANEJO

    Directory of Open Access Journals (Sweden)

    María del Pilar Arroyave

    Full Text Available El objetivo de este artículo es presentar el impacto de los proyectos viales sobre la fauna silvestre y evaluar las medidas de manejo de estos efectos. Se revisó y analizó la información de diversos estudios realizados en el mundo, al igual que algunos estudios de impacto ambiental de carreteras que se han desarrollado en Antioquia. Se encontró que los principales impactos son el atropellamiento, el aislamiento de poblaciones y el cambio en los patrones reproductivos de la fauna; esto trae como consecuencia la disminución de las poblaciones de especies de fauna silvestre. También se plantean las estrategias comúnmente implementadas para el manejo de los impactos.

  7. Uranium enriched granites in Sweden

    International Nuclear Information System (INIS)

    Wilson, M.R.; Aakerblom, G.

    1980-01-01

    Granites with uranium contents higher than normal occur in a variety of geological settings in the Swedish Precambrian, and represent a variety of granite types and ages. They may have been generated by (1) the anatexis of continental crust (2) processes occurring at a much greater depth. They commonly show enrichement in F, Sn, W and/or Mo. Only in one case is an important uranium mineralization thought to be directly related to a uranium-enriched granite, while the majority of epigenetic uranium mineralizations with economic potential are related to hydrothermal processes in areas where the bedrock is regionally uranium-enhanced. (Authors)

  8. Uranium enrichment. 1980 annual report

    International Nuclear Information System (INIS)

    1981-05-01

    This report contains data and related information on the production of enriched uranium at the gaseous diffusion plants and an update on the construction and project control center for the gas centrifuge plant. Power usage at the gaseous diffusion plants is illustrated. The report contains several glossy color pictures of the plants and processes described. In addition to gaseous diffusion and the centrifuge process, three advanced isotope separation process are now being developed. The business operation of the enrichment plants is described; charts on revenue, balance sheets, and income statements are included

  9. Adaptacao transcultural e confiabilidade de medidas de caracteristicas autorreferidas de vizinhanca no ELSA-Brasil

    Directory of Open Access Journals (Sweden)

    Simone M Santos

    2013-06-01

    Full Text Available OBJETIVO: Descrever o processo de adaptação de escalas de medida de características de vizinhança para o português brasileiro. MÉTODOS: As dimensões abordadas foram coesão social, ambiente propício para atividade física, disponibilidade de alimentos saudáveis, segurança em relação a crimes, violência percebida e vitimização. No processo de adaptação foram avaliados aspectos de equivalência entre as escalas originais e respectivas versões para o português. A confiabilidade teste-reteste foi avaliada em submostra de 261 participantes do Estudo Longitudinal de Saúde do Adulto (ELSA-Brasil que responderam ao mesmo questionário em dois momentos distintos em um intervalo de tempo de sete a 14 dias entre as duas aplicações. RESULTADOS: Os aspectos de equivalência avaliados mostraram-se adequados. O coeficiente de correlação intraclasse variou entre 0,83 (IC95% 0,78;0,87 para Coesão Social e 0,90 (IC95% 0,87;0,92 para Ambiente para Atividade Física. As escalas apresentaram consistência interna (alfa de Cronbach que variaram entre 0,60 e 0,84. CONCLUSÕES: As medidas autorreferidas de características de vizinhança tiveram reprodutibilidade muito boa e boa consistência interna. Os resultados sugerem que essas escalas podem ser utilizadas em estudos com população brasileira que apresente características similares àquelas do ELSA-Brasil.

  10. Contribuciones tecnicas para la medida de la contaminacion electromagnetica de microondas. Estudio en diversas poblaciones

    Science.gov (United States)

    Segura Garcia, Jaume

    La contaminacion ambiental por campos electromagneticos ha resultado ser en estos ultimos anos uno de los problemas mas acuciantes del panorama tecnologico y de salud publica. En el primero de los casos porque las inversiones realizadas son enormes y en el segundo porque cada vez es mayor el numero de articulos, "technical reports" e informes medicos que afirman la existencia de una cierta causalidad, aunque sea debil, entre los campos electromagneticos y ciertos cuadros sintomatologicos. En nuestro caso, hemos dedicado bastantes esfuerzos a investigar el llamado "sindrome de radiofrecuencia / microondas", denominado asi en la literatura cientifica por presentarse en operarios de estaciones de radar y en trabajadores de potentes emisoras de radio y television. En esta memoria presentamos un resumen del trabajo realizado durante los ultimos anos en la medida de la contaminacion electromagnetica ambiental asociado a las comunicaciones inalambricas. En ella se recogen los fundamentos fisicos y propiedades de las ondas electromagneticas, la tecnologia empleada en los sistemas de telefonia celular y los antecedentes relativos a la investigacion de la interaccion de las ondas electromagneticas con organismos vivos. Se desarrolla un procedimiento de medida que ha conducido a la elaboracion de los primeros "mapas de radiofrecuencia" similares, en el aspecto descriptivo, a los mapas de ruido desarrollados en el area de la contaminacion acustica. Por ultimo, se analiza la respuesta subjetiva de los residentes, personas que viven en el entorno de cobertura de las estaciones base de telefonia movil y que se ven sometidos a determinados niveles de radiacion electromagnetica, con el fin de situar este fenomeno al nivel que le corresponde en el ambito de la respuesta subjetiva ciudadana.

  11. Sentidos da trajetória de vida para adolescentes em medida de liberdade assistida

    Directory of Open Access Journals (Sweden)

    Clara Costa Gomes

    2014-03-01

    Full Text Available O presente artigo trata de uma pesquisa que teve como objetivo compreender os sentidos atribuídos por adolescentes que cumpriam medida socioeducativa de liberdade assistida às trajetórias de vida e ao envolvimento com infrações à lei. Foram realizados sete sociodramas semanais com 21 adolescentes da comunidade, dos quais 18 eram do sexo masculino. As informações foram organizadas com base no modelo construtivo-interpretativo de González-Rey e analisadas à luz do aporte teórico da Psicossociologia. Da análise resultaram três zonas de sentido: 1 "Parecia uma vida fácil" - referente à sedução do mundo do crime; 2 "Minhas tábuas de salvação" - sobre as estratégias usadas ante os infortúnios da incursão infracional; 3 "A vida no crime não compensa" - concernente ao saldo das escolhas: dores, perdas e arrependimento. As discussões dos encontros desencadearam nos adolescentes relatos acerca do passado, presente e futuro e reflexões que conduzem à reconstrução de projetos de vida desvinculados de atos de infração. Concluiu-se que os métodos socionômico e de história de vida favoreceram a produção de sentidos pelos adolescentes a partir de sua proposta de ação e reflexão e forneceram informações relevantes para se compreender a trajetória infracional dos adolescentes, constituindo-se em poderosa ferramenta de pesquisa-intervenção junto a adolescentes em cumprimento de medidas socioeducativas.

  12. Study of the reduced enrichment fuel conversion at the University of Missouri-Rolla reactor

    International Nuclear Information System (INIS)

    Straka, M.; Bolon, A.; Covington, L.

    1987-01-01

    The method used to analyze the low-enriched uranium core which has been proposed for the University of Missouri-Rolla Reactor is described. Results of calculations for the high-enriched uranium core have been compared with the measured data whenever possible in order to verify this method. For most of the cases that were analyzed the proposed method is adequate and the results obtained for the low-enriched uranium core can be used in revising the licensing documents. (Author)

  13. Prospects and problems of uranium enrichment

    International Nuclear Information System (INIS)

    Imai, Ryukichi

    1974-01-01

    The problem of uranium enrichment now concerns principally peaceful nuclear power generation. With the current oil crisis, energy resources assume unprecedented importance. However, the requirements for enriched uranium vary with the vicissitude of the world situation in nuclear power generation; the enterprise of uranium enrichment is related to economic aspect. The following matters are described: dimension of enrichment problem, political factors, changes in requirements, projects in each country, and strategy of enrichment in Japan. (Mori, K.)

  14. Uranium enrichment in the United States

    International Nuclear Information System (INIS)

    Hill, J.H.; Parks, J.W.

    1975-01-01

    History, improvement programs, status of electrical power availability, demands for uranium enrichment, operating plan for the U. S. enriching facilities, working inventory of enriched uranium, possible factors affecting deviations in the operating plan, status of gaseous diffusion technology, status of U. S. gas centrifuge advances, transfer of enrichment technology, gaseous diffusion--gas centrifuge comparison, new enrichment capacity, U. S. separative work pricing, and investment in nuclear energy are discussed. (LK)

  15. Optimización de la medida de conductividad en membranas poliméricas para pilas de combustible

    OpenAIRE

    Espeso González, Iván

    2016-01-01

    El objetivo de este Trabajo de Fin de Grado se centra en la medida de conductividad iónica de membranas poliméricas de intercambio iónico en diferentes celdas de conductividad para poder optimizar este tipo de medidas y evaluar las ventajas e inconvenientes que presenta cada una de las celdas de conductividad utilizadas para poder ser utilizadas con otro tipo de membranas poliméricas utilizadas también como electrolitos sólidos en pilas de combustible. Se han utilizado co...

  16. Enriched uranium recovery flowsheet improvements

    International Nuclear Information System (INIS)

    Holt, D.L.

    1986-01-01

    Savannah River uses 7.5% TBP to recover and purify enriched uranium. Adequate decontamination from fission products is necessary to reduce personnel exposure and to ensure that the enriched uranium product meets specifications. Initial decontamination of the enriched uranium from the fission products is carried out in the 1A bank, 16 stages of mixer-settlers. Separation of the enriched uranium from the fission product, 95 Zr, has been adequate, but excessive solvent degradation caused by the long phase contact times in the mixer-settlers has limited the 95 Zr decontamination factor (DF). An experimental program is investigating the replacement of the current 1A bank with either centrifugal contactors or a combination of centrifugal contactors and mixer-settlers. Experimental work completed has compared laboratory-scale centrifugal contactors and mixer-settlers for 95 Zr removal efficiencies. Feed solutions spiked with actual plant solutions were used. The 95 Zr DF was significantly better in the mixer-settlers than in the centrifugal contactors. As a result of this experimental study, a hybrid equipment flowsheet has been proposed for plant use. The hybrid equipment flowsheet combines the advantages of both types of solvent extraction equipment. Centrifugal contactors would be utilized in the extraction and initial scrub sections, followed by additional scrub stages of mixer-settlers

  17. Enrichment plant management and safeguards

    International Nuclear Information System (INIS)

    Hurt, N.H.

    1978-01-01

    The next increment of enrichment at Portsmouth will be gas centrifuge. The safeguards program at Portsmouth is discussed, including the DYMCAS system, the computerization, and the detectors. Control of the material access areas is discussed. The licensee material surveillance and verification program is also described

  18. Environmental Development Plan: uranium enrichment

    International Nuclear Information System (INIS)

    1979-09-01

    This Environmental Development Plan identifies and examines the environmental, health, safety, and socioeconomic concerns and corresponding requirements associated with the DOE research, development, demonstration, and operation of the Uranium Enrichment program, including the gaseous diffusion process, the centrifuge process, centrifuge rotor fabrication, and related research and development activities

  19. Applications and theory of electrokinetic enrichment in micro-nanofluidic chips.

    Science.gov (United States)

    Chen, Xueye; Zhang, Shuai; Zhang, Lei; Yao, Zhen; Chen, Xiaodong; Zheng, Yue; Liu, Yanlin

    2017-09-01

    This review reports the progress on the recent development of electrokinetic enrichment in micro-nanofluidic chips. The governing equations of electrokinetic enrichment in micro-nanofluidic chips are given. Various enrichment applications including protein analysis, DNA analysis, bacteria analysis, viruses analysis and cell analysis are illustrated and discussed. The advantages and difficulties of each enrichment method are expatiated. This paper will provide a particularly convenient and valuable reference to those who intend to research the electrokinetic enrichment based on micro-nanofluidic chips.

  20. Capability of the electromagnetic isotope-enrichment facility at ORNL

    International Nuclear Information System (INIS)

    Newman, E.

    1982-01-01

    The isotope separation program at Oak Ridge National Laboratory (ORNL) prepares and distributes electromagnetically enriched stable isotopes to the worldwide scientific community. Among the topics discussed in the present paper are the methods of enriching isotopes, the limitations that apply to the quantity and final assay of the separation products, and a generalized production flowsheet indicating the capability of the facility. A brief description of each of the production steps, from the selection and preparation of initial feedstock to the recovery and distribution of the isotopically enriched material, is presented. The future of the facility, the continued supply of enriched isotopes, and the response of the program to new and changing requirements are emphasized

  1. Successful enrichment of the ubiquitous freshwater acI Actinobacteria.

    Science.gov (United States)

    Garcia, Sarahi L; McMahon, Katherine D; Grossart, Hans-Peter; Warnecke, Falk

    2014-02-01

    Actinobacteria of the acI lineage are often the numerically dominant bacterial phylum in surface freshwaters, where they can account for > 50% of total bacteria. Despite their abundance, there are no described isolates. In an effort to obtain enrichment of these ubiquitous freshwater Actinobacteria, diluted freshwater samples from Lake Grosse Fuchskuhle, Germany, were incubated in 96-well culture plates. With this method, a successful enrichment containing high abundances of a member of the lineage acI was established. Phylogenetic classification showed that the acI Actinobacteria of the enrichment belonged to the acI-B2 tribe, which seems to prefer acidic lakes. This enrichment grows to low cell densities and thus the oligotrophic nature of acI-B2 was confirmed. © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd.

  2. Report of Sectional Committee on Industrialization of Uranium Enrichment

    International Nuclear Information System (INIS)

    1981-01-01

    In order to accelerate the development and utilization of atomic energy which is the core of the substitute energies for petroleum, it is indispensable requirement to establish independent fuel cycle as the base. In particular, the domestic production of enriched uranium is necessary to eliminate the obstacles to secure the energy supply in Japan. The construction and operation of the pilot plant for uranium enrichment by centrifugal separation method have progressed smoothly, and the technical base for the domestic production of enriched uranium is being consolidated. For the time being, the service of uranium enrichment is given by USA and France, but it is expected that the short supply will arise around 1990. The start of operation of the uranium enrichment plant in Japan is scheduled around 1990, and the scale of the plant will be expanded stepwise thereafter. The scale of production is assumed as 3000 t SWU/year in 2000. Prior to this commercial plant, the prototype plant of up to 250 t SWU/year capacity will be operated in 1986, starting the production of centrifugal separators in 1983. The production line for centrifugal separators will have the capacity of up to 125 t SWU/year. The organization for operating these plants, the home production of natural uranium conversion, the uranium enrichment by chemical method and others are described. (Kako, I.)

  3. Influence of environmental enrichment on the behavior and physiology of mice infected by Trypanosoma cruzi

    Directory of Open Access Journals (Sweden)

    Déborah Maria Moreira da Silva

    Full Text Available Abstract INTRODUCTION: Enriched environments normally increase behavioral repertoires and diminish the expression of abnormal behaviors and stress-related physiological problems in animals. Although it has been shown that experimental animals infected with microorganisms can modify their behaviors and physiology, few studies have evaluated how environmental enrichment affects these parameters. This study aimed to evaluate the effects of environmental enrichment on the behavior and physiology of confined mice infected with Trypanosoma cruzi. METHODS: The behaviors of 20 T. cruzi-infected mice and 20 non-infected mice were recorded during three treatments: baseline, enrichment, and post-enrichment. Behavioral data were collected using scan sampling with instantaneous recording of behavior every 30s, totaling 360h. Plasma TNF, CCL2, and IL-10 levels and parasitemia were also evaluated in infected enriched/non-enriched mice. Behavioral data were evaluated by Friedman’s test and physiological data by one-way ANOVA and area under the curve (AUC analysis. RESULTS: Results showed that environmental enrichment significantly increased exploratory behaviors and diminished inactivity. The use of environmental enrichment did not diminish circulating levels of TNF and IL-10 but diminished circulating levels of CCL2 and parasitemia. CONCLUSIONS: Positive behavioral and physiological effects of environmental enrichment were observed in mice living in enriched cages. Thus, environmental enrichment improved the welfare of these animals.

  4. Enrichment of reticulocytes from whole blood using aqueous multiphase systems of polymers.

    Science.gov (United States)

    Kumar, Ashok A; Lim, Caeul; Moreno, Yovany; Mace, Charles R; Syed, Abeer; Van Tyne, Daria; Wirth, Dyann F; Duraisingh, Manoj T; Whitesides, George M

    2015-01-01

    This paper demonstrates the enrichment of reticulocytes by centrifuging whole blood through aqueous multiphase systems (AMPSs)-immiscible phases of solutions of polymers that form step-gradients in density. The interfaces of an AMPS concentrate cells; this concentration facilitates the extraction of blood enriched for reticulocytes. AMPS enrich reticulocytes from blood from both healthy and hemochromatosis donors. Varying the osmolality and density of the phases of AMPS provides different levels of enrichment and yield of reticulocytes. A maximum enrichment of reticulocytemia of 64 ± 3% was obtained from donors with hemochromatosis. When used on peripheral blood from normal donors, AMPS can provide a higher yield of enriched reticulocytes and a higher proportion of reticulocytes expressing CD71 than differential centrifugation followed by centrifugation over Percoll. Blood enriched for reticulocytes by AMPS could be useful for research on malaria. Several species of malaria parasites show a preference to invade young erythrocytes and reticulocytes; this preference complicates in vitro cultivation of these species in human blood. Plasmodium knowlesi malaria parasites invade normal human blood enriched for reticulocytes by AMPSs at a rate 2.2 times greater (P blood. Parasite invasion in normal blood enriched by AMPS was 1.8 times greater (P blood enriched to a similar reticulocytemia by differential centrifugation followed by centrifugation over Percoll. The enrichment of reticulocytes that are invaded by malaria parasites demonstrates that AMPSs can provide a label-free method to enrich cells for biological research. © 2014 Wiley Periodicals, Inc.

  5. Active interrogation of highly enriched uranium

    Science.gov (United States)

    Fairrow, Nannette Lea

    Safeguarding special nuclear material (SNM) in the Department of Energy Complex is vital to the national security of the United States. Active and passive nondestructive assays are used to confirm the presence of SNM in various configurations ranging from waste to nuclear weapons. Confirmation measurements for nuclear weapons are more challenging because the design complicates the detection of a distinct signal for highly enriched uranium. The emphasis of this dissertation was to investigate a new nondestructive assay technique that provides an independent and distinct signal to confirm the presence of highly enriched uranium (HEU). Once completed and tested this assay method could be applied to confirmation measurements of nuclear weapons. The new system uses a 14-MeV neutron source for interrogation and records the arrival time of neutrons between the pulses with a high efficiency detection system. The data is then analyzed by the Feynman reduced variance method. The analysis determined the amount of correlation in the data and provided a unique signature of correlated fission neutrons. Measurements of HEU spheres were conducted at Los Alamos with the new system. Then, Monte Carlo calculations were performed to verify hypothesis made about the behavior of the neutrons in the experiment. Comparisons of calculated counting rates by the Monte Carlo N-Particle Transport Code (MCNP) were made with the experimental data to confirm that the measured response reflected the desired behavior of neutron interactions in the highly enriched uranium. In addition, MCNP calculations of the delayed neutron build-up were compared with the measured data. Based on the results obtained from this dissertation, this measurement method has the potential to be expanded to include mass determinations of highly enriched uranium. Although many safeguards techniques exist for measuring special nuclear material, the number of assays that can be used to confirm HEU in shielded systems is

  6. Avaliação das medidas de oximetria de pulso em indivíduos sadios com esmalte de unha Evaluación de las medidas de oximetría de pulso en individuos sanos con esmalte de uña Evaluation of pulse oximetry measurements in healthy subjects with nail polish

    Directory of Open Access Journals (Sweden)

    Solange Diccini

    2011-01-01

    Full Text Available OBJETIVO: Avaliar as alterações nas medidas da oximetria de pulso em indivíduos sadios com esmaltes de unha. MÉTODOS: Estudo transversal com 80 voluntárias sadias. As cores de esmalte utilizadas para avaliar a saturação periférica de oxigênio (SpO2 foram: café com leite, café, chocolate, vermelho e ameixa. Estas cores foram distribuídas entre as unhas dos dedos da mão esquerda. Os dedos da mão direita foram os controles. RESULTADOS: As cores vermelha (p=0,047 e café (p=0,024 mostraram valores menores na SpO2 quando comparados ao controle. As outras cores não alteraram a medida da SpO2. CONCLUSÃO: As cores vermelha e café causaram redução na medida da SpO2, porém a relevância clínica deste achado é questionável, pois os valores estavam dentro do intervalo de normalidade.OBJETIVO: Evaluar las alteraciones en las medidas de la oximetría de pulso en individuos sanos con esmalte de uña. MÉTODOS: Estudio transversal realizado con 80 voluntarias sanas. Los colores de esmalte utilizados para evaluar la saturación periférica de oxígeno (SpO2 fueron: café con leche, café, chocolate, rojo y ciruela. Estos colores fueron distribuidos entre las uñas de los dedos de la mano izquierda. Los dedos de la mano derecha fueron los controles. RESULTADOS: Los colores rojo (p=0,047 y café (p=0,024 mostraron valores menores en la SpO2 cuando fueron comparados al control. Los otros colores no alteraron la medida del SpO2. CONCLUSIÓN: Los colores rojo y café causaron reducción en la medida del SpO2, sin embargo la relevancia clínica de este hallazgo es cuestionable, pues los valores estaban dentro del intervalo de normalidad.OBJECTIVE: To evaluate the changes in measurements of pulse oximetry in healthy individuals with nail polish. METHODS: Cross sectional study with 80 healthy volunteers. The colors of enamel used to assess oxygen saturation (SpO2 were: coffee with milk, coffee, chocolate, red and plum. These colors were

  7. Feasibility of nondestructive assay measurements in uranium enrichment plants

    Energy Technology Data Exchange (ETDEWEB)

    Walton, R.B.

    1978-04-01

    Applications of nondestructive assay methods to measurement problems in uranium enrichment facilities are reviewed. The results of a number of test and evaluation projects that were performed over the last decade at ORGDP and Portsmouth are presented. Measurements of the residual holdup in the top enrichment portion of the shut-down K-25 cascade were made with portable neutron and gamma-ray detectors, and inventory estimates based on these data were in good agreement with ORGDP estimates. In the operating cascade, the tests showed that portable NaI detectors are effective for monitoring NaF and alumina media for gaseous effluent traps and that gas phase enrichments and inventories, as well as large deposits of uranium, can be detected with portable neutron and gamma-ray instrumentation. A wide variety of scrap and waste materials, including barrier and compressor blades, incinerator ash and trapping media, and miscellaneous waste, were measured using passive gamma-ray and neutron methods and 14-MeV neutron interrogation. Methods developed for rapid verification of UF/sub 6/ in shipping containers with portable neutron and gamma-ray instruments are now used routinely by safeguards inspectors. Passive assay methods can also be used to measure continuously the enrichments of /sup 235/U and /sup 234/U in the UF/sub 6/ product and tails withdrawals of a gaseous diffusion plant. A system that was developed and installed in the extended-range product withdrawal station of the Portsmouth facility measures enrichment with a relative accuracy of 0.5%. A stand-alone neutron detector has also been successfully evaluated for the measurement of the isotopic abundance of /sup 234/U in UF/sub 6/ in sample cylinders, an application of potential importance to Minor Isotope Safeguards Technology. Recommendations are made on the role of NDA measurements for enrichment plant safeguards, including additional tests and evaluations that may be needed, particularly for advanced uranium

  8. Confiabilidade de medidas volumétricas de estruturas temporais mesiais Reliability of mesial temporal lobe volumetric measures

    Directory of Open Access Journals (Sweden)

    Renato L. Marchetti

    2002-06-01

    Full Text Available MOTIVO DO ESTUDO: O desenvolvimento de técnicas confiáveis para a realização de medidas volumétricas de estruturas temporais mesiais (amígdala, hipocampo e giro para-hipocampal em exames de ressonância magnética (RM pode fornecer dados para o estudo de vários transtornos neuropsiquiátricos, particularmente epilepsia do lobo temporal, doença de Alzheimer e esquizofrenia. MÉTODO: Investigamos essas técnicas realizando estudo de confiabilidade intra-observador (IO e entre-observador (EO, envolvendo controles normais, pacientes com epilepsia e pacientes com doença de Alzheimer, através do coeficiente de correlação intra-classe (CCI. RESULTADOS: A confiabilidade IO para as estruturas analisadas variou de 0,93 a 0,99 (pRATIONALE: The development of reliable techniques for volumetric measurement of mesial temporal structures (amygdala, hypocampus and parahypocampal gyrus on magnetic resonance imaging (MRI can provide data for the study of neuropsychiatric disorders, mainly temporal lobe epilepsy, Alzheimer´s disease and schizophrenia. METHOD: We investigated these techniques performing intraobserver and interobserver reliability study concerning normal controls, epilepsy and Alzheimer's disease patients using the intra-class correlation coefficient. RESULTS: Intra-observer reliability of evaluated structures ranged from 0.93 to 0.99 (p<0.001. Inter-observer reliability ranged from 0.70 to 0.95 (p <= 0.001. CONCLUSION: The results suggest that the technique of MRI morphometry of mesial temporal regions can be considered a reliable tool which may help in the investigation of neuropsychiatric disorders, since used by adequately trained clinicians and researchers.

  9. Medidas dinámicas de predictibilidad en el índice s&p 500 y sus determinantes

    Directory of Open Access Journals (Sweden)

    Javier Humberto Ospina Holguín

    2015-04-01

    Full Text Available El objeto de este estudio es medir la predictibilidad del índice accionario estadounidense S&P 500 y establecer posibles determinantes de esta predictibilidad. La predictibilidad se ha estudiado ampliamente, pero en pocos casos mediante medidas dinámicas y per se, como se hace aquí. Para establecer la predictibilidad se utilizan la medida eta del campo de las redes neuronales, el valor p del test de la razón de varianzas del campo de la econometría financiera y el análisis de fluctuación sin tendencia del campo de la econofísica. Se construye un algoritmo de inversión basado en las medidas dinámicas de predictibilidad que es mejor que la estrategia de comprar y mantenerse en el periodo estudiado. Para la medida más promisoria, se establece una relación empírica entre la predictibilidad y varios factores económicos y financieros. El panorama que arroja este estudio es el de un mercado que no es estáticamente predecible, sino cuya predictibilidad evoluciona dinámicamente en el tiempo, lo cual es compatible con la hipótesis del mercado adaptativo.

  10. Medidas Sanitarias y Fitosanitarias, ¿Barreras al Comercio?: el Caso Peruano en el Asia-Pacífico

    Directory of Open Access Journals (Sweden)

    Marcelo Alonso Valverde Arevalo

    2016-01-01

    Full Text Available Los aranceles han dejado de representar la principal barrera al comercio exterior, debido a la reducción constante de éstos en el ámbito multilateral o bilateral. Contrariamente, las medidas no arancelarias han ido en aumento, siendo la principal preocupación para las exportaciones mundiales. En este escenario, se presentan las medidas sanitarias y fitosanitarias, las cuales sirven para proteger la salud de las personas, animales y plantas en contra de riesgos asociados con enfermedades, pestes u otros; sin embargo, por la naturaleza de las mismas pueden distorsionar el comercio hasta ser utilizadas con fines proteccionistas en favor de las industrias nacionales de los países importadores. El presente artículo cuestiona si es que a pesar de contar con una serie de acuerdos comerciales internacionales, los cuales han disminuido progresivamente los aranceles, el Perú ha logrado un acceso real al mercado del Asia-Pacífico con productos agropecuarios y pesqueros. En ese sentido, se presentan casos históricos y pendientes en materia de acceso a mercados por medidas de carácter sanitario o fitosanitario, tal como el acceso de paltas a Japón. Asimismo, se analizan las disposiciones de los acuerdos comerciales en materia de medidas sanitarias y fitosanitarias y se presentan las potenciales oportunidades que se establecen en estos acuerdos, a fin de agilizar el acceso a mercados internacionales, y que no se han aprovechado.

  11. As Inundações e Memória Social: Algumas Medidas De Prevenção ...

    African Journals Online (AJOL)

    As Inundações e Memória Social: Algumas Medidas De Prevenção e Controlo da Biodegradação Dos Documentos e Arquivos (Floods and Social Memory: Some Measures for the Protection and Control of Biodegradation in Records and Archives)

  12. Medidas que puede tomar para prevenir el cáncer (Steps You Can Take to Prevent Cancer)

    Centers for Disease Control (CDC) Podcasts

    2012-02-02

    Este podcast habla de las principales medidas que las personas pueden tomar para reducir su riesgo de contraer cáncer.  Created: 2/2/2012 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 3/13/2012.

  13. Enrichment into the 21st century

    International Nuclear Information System (INIS)

    Rutkowski, E.

    1995-01-01

    This article discusses the future of the enrichment services market into the next century. It is estimated that demand for enrichment services will reach 31 million SWU by the end of the century and remain constant for the following 10 years. The current world enrichment capacity is 44 million SWU, or some 50% ahead of the demand. This oversupply is projected to continue into the next century, but in spite of this, several suppliers are planning new enrichment facilities. HEU as a source of enriched uranium is examined. Overall, long-term prices for enrichment services are expected to decline in the coming decade

  14. Uranium Enrichment Determination of the InSTEC Sub Critical Ensemble Fuel by Gamma Spectrometry

    International Nuclear Information System (INIS)

    Borrell Munnoz, Jose L.; LopezPino, Neivy; Diaz Rizo, Oscar; D'Alessandro Rodriguez, Katia; Padilla Cabal, Fatima; Arbelo Penna, Yunieski; Garcia Rios, Aczel R.; Quintas Munn, Ernesto L.; Casanova Diaz, Amaya O.

    2009-01-01

    Low background gamma spectrometry was applied to analyze the uranium enrichment of the nuclear fuel used in the InSTEC Sub Critical ensemble. The enrichment was calculated by two variants: an absolute method using the Monte Carlo method to simulated detector volumetric efficiency, and an iterative procedure without using standard sources. The results confirm that the nuclear fuel of the ensemble is natural uranium without any additional degree of enrichment. (author)

  15. Impacto sonoro do aeroporto Santos Dumont/RJ: análise e medidas de controle

    Directory of Open Access Journals (Sweden)

    José R. de Lima Neto

    2014-09-01

    Full Text Available A poluição sonora é parte cotidiana da vida nas grandes cidades. Dentre as fontes sonoras relevantes no contexto das cidades, tais como o ruído viário, uma que oferece desafios particulares é a relacionada ao sobrevoo de aeronaves, sejam aviões comerciais, jatos particulares ou helicópteros. Por se tratar de fontes intensas de ruído e das características das suas trajetórias (extensas e acima das habitações, tanto a avaliação do incômodo gerado, como eventuais medidas de mitigação são complexas e de difícil implementação.Dessa forma, o desconforto acústico gerado nas comunidades próximas as rotas de pouso e decolagem de grandes aeroportos tornou-se alvo de grandes reclamações por parte dos moradores que tem seu conforto ameaçado, o que reforça a necessidade de enfrentar estes desafios e atacar este problema.Assim, nesse trabalho, buscamos avaliar o impacto sonoro das aeronaves que possuem como destino ou são oriundas do aeroporto Santos Dumont/RJ (SBRJ, através de simulação computacional de ruído, em uma região próxima ao aeroporto com área de aproximadamente 39 km². A partir da quantificação e determinação das áreas efetivamente afetadas pelo ruído aeronáutico, e da comparação dos valores obtidos com as normas da legislação brasileira, esse trabalho visa mapear possíveis regiões de conflito e fornecer informações que orientem possíveis medidas mitigadoras e de controle de ruído plausíveis para a realidade atual. Palavras-Chave: Acústica, Ruído aeronáutico, Impacto Sonoro de Aeroportos.

  16. Medidas de biossegurança adotadas por profissionais atuantes em audiologia

    Directory of Open Access Journals (Sweden)

    Ana Paula Ferreira Rocha

    2015-03-01

    Full Text Available OBJETIVO: explicitar as práticas de biossegurança adotadas por fonoaudiólogos atuantes na área de Audiologia e relacioná-las com a educação continuada e o tempo de formação dos profissionais. MÉTODOS: participaram deste estudo 70 fonoaudiólogos atuantes na área de Audiologia, nos municípios de Belo Horizonte e Contagem. Foi aplicado um questionário sobre biossegurança em Audiologia, com base na Norma Regulamentadora 32, composto por 27 perguntas fechadas, abordando os aspectos de higienização das mãos, equipamentos de proteção individual e organização e higienização dos artigos e do ambiente. RESULTADOS: a maioria dos fonoaudiólogos relatou que adota as seguintes medidas de biossegurança: higienização das mãos antes dos atendimentos (71%, uso de jaleco com mangas longas (74% e abotoado (91%, cabelos presos (79%, unhas limpas e cortadas (91%, separação e desinfecção dos artigos usados (83% e organização do ambiente (97%. No entanto somente 40% dos profissionais referiram higienizar as mãos entre os atendimentos e 9% referiram o uso de luvas na realização da meatoscopia. Observou-se que a destinação dos artigos para desinfecção é uma prática mais rotineira para profissionais com especialização (p< 0,05. Os profissionais com maior tempo de formação aderiram melhor à prática de higienização das mãos. CONCLUSÃO: higienizar as mãos antes dos atendimentos, vestir-se corretamente, destinar os artigos para desinfecção e organizar o ambiente de trabalho são práticas de biossegurança adotadas pela maioria dos fonoaudiólogos. Fatores como tempo de formação e educação continuada influenciam de maneira positiva na adoção de corretas medidas de biossegurança.

  17. Un criterio robusto para la medida del margen –coeficiente– de seguridad

    Directory of Open Access Journals (Sweden)

    Cervera, J.

    2010-06-01

    Full Text Available This paper offers a brief analysis of the drawbacks that can be found with the usual partial security coefficients, as loads multipliers, when employed for the measurement of the distance to the collapse condition in such structures, as masonry arches, that have important anisotropies or lacks tensile strength. To deal with such difficulties the paper presents a simple and robust security criterium based on a contraction of the yield surface. That criterium, that performs identically in isotropic structures to those usually employed, can however deal consistently with situations where former criteria fail. The criterium is applied to sections with double symmetry under axial load and unsymmetrical bending, in materials with and without tension resistance. It is also applied to the prism stability problem, showing the advantages in the caracterization of such problems.

    En este trabajo se analizan en primer lugar algunos inconvenientes de los habituales coeficientes parciales de seguridad —factores multiplicadores de las cargas— como medida del alejamiento a las condiciones de colapso en el caso de las estructuras con anisotropías importantes, como son especialmente aquellas sin resistencia a la tracción —los arcos de fábrica, como ejemplo paradigmático— Para superar dichas dificultades se propone un criterio sencillo y robusto basado en la contracción de la superficie límite, criterio que aporta una medida coincidente con la habitual para los casos isótropos, pero que permite medir igualmente de forma consistente el alejamiento a las condiciones del colapso para el resto de las situaciones. Se aplica finalmente el modelo a la determinación de los márgenes de seguridad en secciones de doble simetría sometidas a compresión esviada para materiales sin, y con resistencia a tracción, así como al problema de la estabilidad de un prisma frente al vuelco, mostrando las mejoras que aporta para caracterizar dichos problemas.

  18. Enrichment demand boosts SWU prices

    International Nuclear Information System (INIS)

    Anon.

    1993-01-01

    The enrichment market is picking up significantly on very brisk demand. US utilities, which normally purchase material nine months to a year ahead of time, are already hitting the market to fill their 1996 requirements. In June, two non-US utilities, one European entity and a US utility bought SWUs, the entity in an off-market deal. But that doesn't tell the whole story. Three other US utilities entered the market during the month. Meanwhile, we count 13 more utilities getting ready to hit the market for more than 4 million SWUs. Why the surge in demand? Utilities, uncertain of the role to be played by the new US Enrichment Corp. and seeking to take advantage of low interest rates, are implementing buy and hold strategies. As a result, the upper end of NUKEM's SWU price range inched up to $78. The lower end dipped to $67 based on the European deal

  19. Medida da acuidade visual em um shopping center metropolitano Visual acuity measurement in a metropolitan shopping mall population

    Directory of Open Access Journals (Sweden)

    Giovanni M. Travi

    2000-04-01

    Full Text Available Objetivo: Determinar a prevalência de baixa acuidade visual (AV em uma amostra populacional, com um padrão socioeconômico diferenciado. Métodos: Realizou-se estudo de prevalência, sendo verificada a AV de 213 voluntários examinados em um posto no interior de um shopping center em Porto Alegre, por meio do aparelho Ortho-Rater, da Bausch & Lomb. Foi medida a AV para longe em todos os indivíduos e naqueles com 40 anos ou mais, a AV para perto, ambos sem e com correção. Determinou-se como baixa AV, aquela inferior a 10 (equivalente a 20/20 na Tabela de Snellen. Resultados: A idade variou de 6 a 75 anos, com uma média ± desvio-padrão de 27,8 ± 15,16 anos, pertencendo a maioria dos examinados à faixa entre 10 e 40 anos. Encontrou-se uma prevalência de 38,7% (IC 95%: 32,1-45,7 de baixa AV para longe nos dois olhos. 24,9% (IC 95%: 19,2-31,3 apresentavam déficit visual binocular e não faziam uso de correção. Para perto, observou-se que 70,8% (IC 95%: 55,9-83 dos indivíduos testados apresentaram déficit visual binocular sem correção e 41,7% (IC 95%: 27,6-56,8 apresentaram baixa AV independente do uso de correção. Conclusão: Este estudo demonstrou que existe considerável parcela da população com déficit visual, necessitando de avaliação e manejo adequados. Conclui-se que programas de medida da AV e orientação sobre saúde ocular são importantes em qualquer ambiente e atingindo diferentes classes socioeconômicas.Purpose: To determine the prevalence of low visual acuity (VA in a populational sample, with a differentiated social pattern. Methods: This was a prevalence study, in which the 213 volunteers' VA, examined in a shopping mall in Porto Alegre, was verified through the Ortho-Rater device, from Bausch & Lomb. Far sight VA was measured in all the individuals and in those who were 40 years old or older, near sight VA, both of them without and with correction. VA was considered low when it was less than 10 (equivalent

  20. Enrichment techniques employed in phosphoproteomics

    Czech Academy of Sciences Publication Activity Database

    Fíla, Jan; Honys, David

    2012-01-01

    Roč. 43, č. 3 (2012), s. 1025-1047 ISSN 0939-4451 R&D Projects: GA ČR(CZ) GAP501/11/1462; GA ČR GA522/09/0858; GA ČR GA525/09/0994; GA MŠk OC08011 Institutional research plan: CEZ:AV0Z50380511 Keywords : Phosphoproteomics * Enrichment * IMAC Subject RIV: ED - Physiology Impact factor: 3.914, year: 2012

  1. Uranium enrichment plans and policies

    International Nuclear Information System (INIS)

    Schwennesen, J.L.

    1981-01-01

    Significant progress has been made in US efforts to expand its enrichment capacity. The Cascade Improvement Program (CIP) and Cascade Upgrading Program (CUP) are now complete at Oak Ridge and Paducah and almost complete at Portsmouth. Considerable progress has also been made in constructing the Gas Centrifuge Enrichment Plant (GCEP), and physical construction of the first process building is well under way. Current plans are to have two process buildings on-line by 1989 with the remaining six buildings to be added sequentially as needed to meet demand. The status of DOE enrichment services contracts is essentially unchanged from that reported at last year's seminar. The OUEA latest forecast of nuclear power growth, however, is considerably lower than reported last year, although a leveling trend is becoming apparent. The Variable Tails Assay Option (VTAO) of the AFC contract was made available for the third time for FY 1983. The DOE inventories of natural uranium still remain high. The Department of Energy will dispose of this material by using it for Government programs and for enrichment plant operations. It appears that Government inventories of uranium are adequate through at least the mid-1990s. It remains DOE policy not to dispose of its natural uranium stocks through direct sales in the marketplace, except for very small quantities or if an emergency situation would exist and all reasonable attempts had been made, without success, to obtain natural uranium from commercial sources. Finally, with regard to DOE plans on future transaction tails assays, it still appears likely that the current 0.20 percent uranium-235 reference tails assay will be maintained until well into the 1990s, at which time it might be increased up to 0.25 percent uranium-235

  2. Selenium Enrichment of Horticultural Crops.

    Science.gov (United States)

    Puccinelli, Martina; Malorgio, Fernando; Pezzarossa, Beatrice

    2017-06-04

    The ability of some crops to accumulate selenium (Se) is crucial for human nutrition and health. Selenium has been identified as a cofactor of the enzyme glutathione peroxidase, which is a catalyzer in the reduction of peroxides that can damage cells and tissues, and can act as an antioxidant. Plants are the first link in the food chain, which ends with humans. Increasing the Se quantity in plant products, including leafy and fruity vegetables, and fruit crops, without exceeding the toxic threshold, is thus a good way to increase animal and human Se intake, with positive effects on long-term health. In many Se-enriched plants, most Se is in its major organic form. Given that this form is more available to humans and more efficient in increasing the selenium content than inorganic forms, the consumption of Se-enriched plants appears to be beneficial. An antioxidant effect of Se has been detected in Se-enriched vegetables and fruit crops due to an improved antioxidative status and to a reduced biosynthesis of ethylene, which is the hormone with a primary role in plant senescence and fruit ripening. This thus highlights the possible positive effect of Se in preserving a longer shelf-life and longer-lasting quality.

  3. Selenium Enrichment of Horticultural Crops

    Directory of Open Access Journals (Sweden)

    Martina Puccinelli

    2017-06-01

    Full Text Available The ability of some crops to accumulate selenium (Se is crucial for human nutrition and health. Selenium has been identified as a cofactor of the enzyme glutathione peroxidase, which is a catalyzer in the reduction of peroxides that can damage cells and tissues, and can act as an antioxidant. Plants are the first link in the food chain, which ends with humans. Increasing the Se quantity in plant products, including leafy and fruity vegetables, and fruit crops, without exceeding the toxic threshold, is thus a good way to increase animal and human Se intake, with positive effects on long-term health. In many Se-enriched plants, most Se is in its major organic form. Given that this form is more available to humans and more efficient in increasing the selenium content than inorganic forms, the consumption of Se-enriched plants appears to be beneficial. An antioxidant effect of Se has been detected in Se-enriched vegetables and fruit crops due to an improved antioxidative status and to a reduced biosynthesis of ethylene, which is the hormone with a primary role in plant senescence and fruit ripening. This thus highlights the possible positive effect of Se in preserving a longer shelf-life and longer-lasting quality.

  4. The isotopic enrichment of uranium in 1979

    International Nuclear Information System (INIS)

    Baron, M.

    1979-01-01

    The Eurodif uranium enrichment plant built on the Tricastin site is described. The uranium isotope separation plants in service abroad are presented. The main characteristics of the international enrichment market are defined [fr

  5. Uranium enrichment (a strategy analysis overview)

    International Nuclear Information System (INIS)

    Blahnik, C.

    1979-08-01

    An analysis of available information on enrichment technology, separative work supply and demand, and SWU cost is presented. Estimates of present and future enrichment costs are provided for use in strategy analyses of alternate nuclear fuel cycles and systems. (auth)

  6. Curriculum enrichment through indigenous Zulu games | Roux ...

    African Journals Online (AJOL)

    Curriculum enrichment through indigenous Zulu games. ... 1997). The aim of the study was to document and analyze indigenous Zulu games for possible curriculum enrichment of physical ... AJOL African Journals Online. HOW TO USE AJOL.

  7. Validación de la Escala CISNEROS de Medida de Mobbing al Gallego

    Directory of Open Access Journals (Sweden)

    M.ª Ángeles López-Cabarcos

    2009-01-01

    Full Text Available El objetivo del trabajo es la validación de la escala CISNEROS de medida de mobbing en gallego, formada por 43 ítems que miden la frecuencia de mobbing a través de una escala de 7 puntos, al tiempo que permite identificar a los posibles responsables del mismo. Para ello se llevó a cabo un análisis factorial exploratorio y un análisis factorial confirmatorio en una muestra multisectorial representativa de la población activa de Galicia. Los resultados del análisis factorial exploratorio indican la existencia de cinco factores principales que explican el 60,31% de la varianza total y muestran una elevada fiabilidad de la escala (alfa=.96. Posteriormente, se compara el ajuste de tres modelos mediante análisis factorial confirmatorio. Los resultados indican que el modelo de cinco factores correlacionados es el que mejor se ajusta a los datos, dando así apoyo empírico a la estructura de cinco factores obtenida en el análisis factorial exploratorio.

  8. Índices de seguridad de voltaje calculados con datos de unidades fasoriales de medida

    Directory of Open Access Journals (Sweden)

    Carlos J. Zapata

    2009-11-01

    Full Text Available Las unidades de medida fasorial son un recurso tecnológico que permite observar con gran precisión el estado dinámico del sistema eléctrico de potencia. Su aplicación permite, entre muchas otras cosas, implementar índices para evaluar en tiempo real la seguridad de voltaje. Este artículo presenta una breve historia del desarrollo de estos índices y una propuesta inicial de escalas semánticas - gráficas para tres de ellos, con el fin de expresar en forma cualitativa al operador del centro de control el estado de la seguridad de voltaje del sistema./ Phasor measurement units are a technological resource that allows observing with great accuracy the dynamic state of the electric power system. Its application allows, among many things, the implementation of indexes for the on line assessment of voltage security. This paper presents a brief review on the development of these indexes and the results of a study on the establishment semantic - graphic scales for three of them which express to the control center operator in a qualitative way the state of system voltage security

  9. Obtención de una medida del consumo nacional de comidas.

    Directory of Open Access Journals (Sweden)

    Álvarez Martínez, Pedro

    2004-01-01

    Full Text Available En España el gasto de alimentos representa un alto porcentaje del total de presupuesto de los hogares. El presupuesto se reparte como sigue: carne (29%, frutas, legumbres, veduras y patatas (19%, productos lácteos, huevos y quesos (14%, pan y cereales (13%, pescado (13%, grasas y aceites (4%, otros alimentos (8%. El consumo de alimentos está afectado no solo por factores económicos sino también por hábitos, gustos, antecedentes culturales y forma de vida. Por tanto un importante un objetivo importante para los investigadores es medir la importancia relativa de estos factores, y cuales de ellos determinan la preferencia. Estas preferencias son diferentes para gente que viven en ciudades de distintas población. Este hecho es debido a diferentes antecedentes culturales, factores sociales, y cambios de sistemas de distribución. El objetivo de este trabajo es aplicar una nueva metodología que nos permita obtener una medida de las preferencias en el consumo de los alimentos conociendo solo la cantidad de dinero semanal que los hogares gastan en cada una de los alimentos, y detectar así posibles hábitos de consumo.

  10. El fracaso escolar y los enfoques de aprendizaje: medidas para la inclusión educativa

    Directory of Open Access Journals (Sweden)

    Ana Belén Mirete Ruiz

    2015-01-01

    Full Text Available El índice de fracaso escolar existente actualmente en España resulta muy preocupante a pesar de las numerosas reformas educativas que se han llevado a cabo en los últimos años. Para analizar los factores motivacionales y de estrategias de aprendizaje que pueden estar incidiendo en la calidad del aprendizaje de los estudiantes se ha realizado una investigación con 281 estudiantes de Educación Secundaria Obligatoria a los que se ha aplicado el Cuestionario sobre Procesos de Aprendizaje (CPA. También se les ha planteado una serie de cuestiones sobre aspectos relacionados con la inclusión, los factores que pueden estar influyendo en el fracaso escolar y las medidas de mejora del rendimiento académico aplicadas por los estudiantes según sus enfoques de aprendizaje. Con ello se ha podido identificar las características de los aprendizajes de este grupo de estudiantes y se han planteado una serie de propuestas de mejora que de ser aplicadas podrían repercutir en la calidad de sus aprendizajes, en la mejora de su rendimiento académico y en minimizar los nefastos efectos del fracaso escolar en este nivel educativo.

  11. RELAÇÃO DA FOLGA ORGANIZACIONAL COM MEDIDAS DE DESEMPENHO DE EMPRESAS BRASILEIRAS

    Directory of Open Access Journals (Sweden)

    Ilse Maria Beuren

    2015-07-01

    Full Text Available O estudo objetiva identificar a relação da folga organizacional (potencial, inabsorvida e absorvida com medidas de desempenho (ROA, ROE e ROI em empresas brasileiras. A pesquisa descritiva com abordagem quantitativa foi realizada utilizando dados financeiros disponíveis na base de dados Economática@. A população da pesquisa compreendeu as 500 Maiores e Melhores empresas de 2010 segundo a Revista Exame; e a amostra as 119 listadas na BM&FBovespa. Os resultados mostram que as empresas apresentaram folga organizacional, na ordem folga potencial, absorvida e inabsorvida. Os modelos de análise indicaram relação significativa entre as variáveis ROA, ROE, ROI e as variáveis folga potencial, absorvida, inabsorvida. No entanto, a folga potencial, analisada pelos indicadores ROE e ROI, apresentou sinal negativo, logo, quanto maior a folga, menor o respectivo índice de desempenho. Observou-se ainda uma tendência da folga potencial ser inversa ao desempenho da empresa, com exceção do índice de desempenho ROI, que não apresentou significância.

  12. Enriched uranium sales: effect on supply industry

    International Nuclear Information System (INIS)

    Andersen, R.K.

    1985-01-01

    The subject is covered in sections: introduction (combined effect of low-enriched uranium (LEU) inventory sales and utility services enrichment contract terms); enrichment market overview; enrichment market dynamics; the reaction of the US Department of Energy; elimination of artificial demand; draw down of inventories; purchase and sale of LEU inventories; tails assay option; unfulfilled requirements for U 3 O 8 ; conclusions. (U.K.)

  13. Cheese bread enriched with biofortified cowpea flour

    Directory of Open Access Journals (Sweden)

    Rodrigo Barbosa Monteiro Cavalcante

    2016-02-01

    Full Text Available ABSTRACT The development and enrichment of food are of great importance not only for the industry but also to improve the population's nutrition, where you can create new products or optimize existing ones. The consumption of fortified products in the diet is an option for the control of deficiency diseases. This study aimed to develop enriched cheese bread with whole biofortified cowpea flour and evaluate their acceptance and chemical composition. Two formulations, F1 and F2, were prepared containing 5.6 and 8% of cowpea flour as a substitute for starch, respectively. To check acceptance, three sensory tests were used (Hedonic Scale, Purchase Intent, and Paired Comparison, F1 being sensory viable according to assessors, being chemically analyzed. Minerals were determined by atomic emission spectrometry with inductively coupled plasma source. The moisture was determined by drying at 105 °C, ash by calcination in muffle at 550 °C, proteins by the macro-Kjeldahl method, and lipids by hot extraction in a Soxhlet extractor. Carbohydrates were obtained by difference and the calories were calculated. The addition of cowpea increased the amounts of copper, iron, phosphorus, magnesium, manganese, and zinc, as well as protein and carbohydrate values. On the other hand, there was a reduction of the moisture concerning lipids and the total caloric value compared to the standard formulation. It was concluded , therefore, that the cowpea, a regional raw material in market expansion is presented as an option for the enrichment of baked foods that do not contain gluten, such as cheese bread.

  14. 31 CFR 540.316 - Uranium enrichment.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Uranium enrichment. 540.316 Section 540.316 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... REGULATIONS General Definitions § 540.316 Uranium enrichment. The term uranium enrichment means the process of...

  15. Method for selective recovery of PET-usable quantities of [.sup.18 F] fluoride and [.sup.13 N] nitrate/nitrite from a single irradiation of low-enriched [.sup.18 O] water

    Science.gov (United States)

    Ferrieri, Richard A.; Schlyer, David J.; Shea, Colleen

    1995-06-13

    A process for simultaneously producing PET-usable quantities of [.sup.13 N]NH.sub.3 and [.sup.18 F]F.sup.- for radiotracer synthesis is disclosed. The process includes producing [.sup.13 N]NO.sub.2.sup.- /NO.sub.3.sup.- and [.sup.18 F]F.sup.- simultaneously by exposing a low-enriched (20%-30%) [.sup.18 O]H.sub.2 O target to proton irradiation, sequentially isolating the [.sup.13 N]NO.sub.2.sup.- /NO.sub.3.sup.- and [.sup.18 F]F.sup.- from the [.sup.18 O]H.sub.2 O target, and reducing the [.sup.13 N]NO.sub.2.sup.- /NO.sub.3.sup.- to [.sup.13 N]NH.sub.3. The [.sup.13 N]NH.sub.3 and [.sup.18 F]F.sup.- products are then conveyed to a laboratory for radiotracer applications. The process employs an anion exchange resin for isolation of the isotopes from the [.sup.18 O]H.sub.2 O, and sequential elution of [.sup.13 N]NO.sub.2.sup.- /NO.sub.3.sup.- and [ .sup.18 F]F.sup.- fractions. Also the apparatus is disclosed for simultaneously producing PET-usable quantities of [.sup.13 N]NH.sub.3 and [.sup.18 F]F.sup.- from a single irradiation of a single low-enriched [.sup.18 O]H.sub.2 O target.

  16. As medidas aplicadas aos acordos SPS e TBT sobre as exportações brasileiras de fumo

    Directory of Open Access Journals (Sweden)

    Carlos Otávio de Freitas

    2015-11-01

    Full Text Available O presente estudo tem como objetivo analisar os efeitos das medidas não tarifárias, especificamente as medidas sanitárias e fitossanitárias - SPS e medidas técnicas - TBT, sobre as exportações brasileiras de fumo e se tais efeitos permaneceram nos períodos subsequentes à emissão das notificações. Para tal, foi estimado um modelo gravitacional utilizando uma amostra de 89 países importadores do fumo do Brasil entre o período de 1997 a 2011. O método utilizado na estimação das equações foi o modelo Poisson-Pseudo Maximum Likehood-PPML. Os resultados obtidos mostram que as medidas ou SPS afetaram negativamente o fluxo internacional do comércio de fumo. Além disso, verificou-se que esses efeitos perduraram nos períodos posteriores à emissão da notificação, sendo que as empresas exportadores podem demorar até dois anos para se ajustarem às exigências requeridas. Dentre os objetivos das notificações SPS, a maior parte está relacionada a medidas para proteção da saúde humana e proteção da planta, o que pode explicar a maior dificuldade de adequação, pois são exigências que demandam grandes investimentos. Já as medidas TBT não foram estatisticamente significativas, confirmando o caráter não restritivo dessas notificações sobre o comércio internacional de fumo. Apesar da maior parte das notificações ao fumo estarem associadas a medidas técnicas, os resultados indicaram que elas não configuraram como barreiras ao comércio de fumo. Isso pode sugerir que as exigências a elas referidas, que, em sua grande maioria, foi referente à rotulagem e/ou embalagem, foram atendidas pelo Brasil sem maiores dificuldades. Dessa forma, torna-se importante a inclusão de questões referentes à regulamentação dessas medidas nas discussões e processos de tomada de decisão das empresas exportadoras de fumo. Em termos de ações governamentais, seria interessante a criação ou aperfeiçoamento de políticas no sentido

  17. Uranium enrichment by jet nozzle separation process in the German-Brazil cooperation program

    International Nuclear Information System (INIS)

    Becker, E.W.

    1991-01-01

    It presents a lecture on technical and commercial aspects of uranium isotopic enrichment by the Get Lozzle Method in Brazil. The analysis is presented regarding the context of bilateral agreement German-Brazil it discusses the technical problems of a demonstration plant design as well as the commercial exportation viability of enriched uranium produced in Brazil by the Jet Nozzle Method. (author)

  18. USO DE MEDIDAS AUTO-REFERIDAS PARA AVALIAÇÃO DO ESTADO NUTRICIONAL DE ADOLESCENTES

    Directory of Open Access Journals (Sweden)

    ROZANE APARECIDA TOSO BLEIL

    2009-12-01

    Full Text Available

    Comparar os valores referidos e aferidos de peso e estatura em estudantes adolescentes de um município da região oeste paranaense. Participaram do estudo 125 adolescentes com idade entre 10 e 18 anos. Os estudantes relataram aos pesquisadores dados referentes ao peso e estatura e, após o registro das informações, os jovens foram pesados e medidos. Foram coletadas informações sobre a percepção corporal para verifi car como o adolescente percebe seu peso em relação a sua estatura e as respostas foram agrupadas em três categorias. O teste t-student pareado foi utilizado para identifi car possíveis diferenças nas medidas referidas e aferidas de peso, estatura e do Índice de Massa Corporal - IMC. Para avaliar a concordância entre as medidas aferidas e referidas, calculou-se o coefi ciente de correlação intraclasse de Pearson (CCI, de acordo com a faixa etária e o gênero de cada indivíduo. O teste t-student foi adotado para verifi car possíveis diferenças entre os resultados obtidos, de acordo com o gênero dos alunos. Adotouse o nível de signifi cância de 5%. Os resultados obtidos mostraram que as meninas mais jovens apresentaram melhor percepção corporal quando comparadas às alunas com maior idade. Não foram identifi cadas diferenças signifi cativas entre as medidas referidas e aferidas do grupo estudado, em relação ao gênero e à faixa etária. Porém, nota-se que osadolescentes tendem a subestimar suas medidas corporais, sendo a estatura, a que apresentou menor concordância entreos valores informados e aferidos. As medidas referidas devem ser utilizadas com cuidado na avaliação nutriciona lde adolescentes. Sugere-se a implementação de outros estu dospara confi rmar a validade do uso de tais medidas e ainda avaliar outros fatores que possam interferir nos resulta dos.

  19. Altura do joelho como medida alternativa confiável na avaliação nutricional de idosos

    Directory of Open Access Journals (Sweden)

    Vera Elizabeth CLOSS

    2015-10-01

    Full Text Available Objetivo:Avaliar o uso da altura do joelho como medida alternativa confiável na avaliação nutricional de idosos atendidos em um ambulatório de geriatria.Métodos:Estudo transversal com idosos atendidos em ambulatório de geriatria de um hospital universitário, entre novembro de 2009 e novembro de 2010. Variáveis investigadas: gênero, idade, peso, estatura, altura do joelho, estatura estimada através das equações de Chumlea, índice de massa corporal para as medidas de estatura aferida e estimada e classificado segundo Lipschitz, e dados sociodemográficos. Na análise descritiva dos dados foram usadas medidas de tendência central, dispersão e proporção; a homogeneidade entre as medidas foi verificada através do coeficiente de correlação intraclasse e do gráfico de Bland e Altman e a concordância entre a avaliação nutricional baseada no índice de massa corporal calculado a partir da estatura aferida e a avaliação nutricional baseada no índice de massa corporal calculado a partir da estatura estimada foi avaliada através do coeficiente Kappa ponderado com pesos quadráticos.Resultados:Foram avaliados 186 idosos com média de idade de 74,3±7,1 anos. A média do índice de massa corporal aferido e estimado foi 28,96±5,86 kg/m2e 27,95±5,47 kg/m2, respectivamente, e a prevalência de sobrepeso foi de 59,7% para o índice de massa corporal aferido e 53,2% para o estimado. Foi observada uma boa concordância (Kappa=0,79; p<0,001 entre as duas avaliações.Conclusão:Os resultados mostraram ser possível utilizar a altura do joelho na estimativa da estatura para cálculo do índice de massa corporal em idosos atendidos em ambulatório, alternativa útil para aqueles que apresentam limitações na aferição das medidas, sendo esta uma medida alternativa importante na avaliação nutricional de idosos.

  20. Glycoprotein Enrichment Analytical Techniques: Advantages and Disadvantages.

    Science.gov (United States)

    Zhu, R; Zacharias, L; Wooding, K M; Peng, W; Mechref, Y

    2017-01-01

    Protein glycosylation is one of the most important posttranslational modifications. Numerous biological functions are related to protein glycosylation. However, analytical challenges remain in the glycoprotein analysis. To overcome the challenges associated with glycoprotein analysis, many analytical techniques were developed in recent years. Enrichment methods were used to improve the sensitivity of detection, while HPLC and mass spectrometry methods were developed to facilitate the separation of glycopeptides/proteins and enhance detection, respectively. Fragmentation techniques applied in modern mass spectrometers allow the structural interpretation of glycopeptides/proteins, while automated software tools started replacing manual processing to improve the reliability and throughput of the analysis. In this chapter, the current methodologies of glycoprotein analysis were discussed. Multiple analytical techniques are compared, and advantages and disadvantages of each technique are highlighted. © 2017 Elsevier Inc. All rights reserved.

  1. 76 FR 11523 - Atomic Safety and Licensing Board; AREVA Enrichment Services, LLC (Eagle Rock Enrichment Facility...

    Science.gov (United States)

    2011-03-02

    ... and Licensing Board; AREVA Enrichment Services, LLC (Eagle Rock Enrichment Facility); Notice of... Governmental Entities Regarding Environmental Portion of Enrichment Facility Licensing Proceeding February 24.... White. In this 10 CFR part 70 proceeding regarding the request of applicant AREVA Enrichment Services...

  2. Enriching Orphans’ Potentials through Interpersonal and Intrapersonal Intelligence Enrichment Activities

    Directory of Open Access Journals (Sweden)

    Nurulwahida Hj Azid

    2016-01-01

    Full Text Available Orphans are considered a minority and they should be given a greater emphasis so that they do not feel left out and can build their own lives without a sense of humility. This does not mean that the orphans should be pampered instead they should be given the confidence and motivation to strive for success in later life. Humility among orphans can be associated with interpersonal and intrapersonal intelligences. This study aims to evaluate the impact of problem-solving activity treatment based on the interpersonal and intrapersonal intelligences. 46 students from two orphanages were involved as the treatment group. The research design used was a one-group pretest-posttest design applied through a combination of quantitative and qualitative approaches. Enrichment activities that provided interpersonal and intrapersonal skills as evidenced in this study should be carried out regularly at orphanages. Our study has proven that orphans‟ rights to learn cannot be neglected and „no child left behind „policy needs to be carried through by everybody involved with orphans‟ well-being. Teachers and carers need to be trained to use these enrichment activities at their orphanages to help maximize the orphans‟ potentials.

  3. The reduced enrichment program for JRR-4

    International Nuclear Information System (INIS)

    Takayanagi, M.

    1992-01-01

    Japan Research Reactor No. 4(JRR-4) with the rated power of 3.5 MW, swimming pool type research reactor, 93 % enriched uranium ETR-type fuel used, light water moderated and cooled. The first criticality reached on 28th January, 1965. The reactor has operated for about 26 years. However, it was planed to the reduced enrichment of the fuels to low enrichment according to the International Reduced Enrichment for Research and Test Reactors (RERTR) program. This paper describes the program for conversion of the enrichment of fuel from 93 % to less than 20 %. (author)

  4. Método del “Pulso Láser” para la medida de la difusividad térmica en materiales cerámicos

    Directory of Open Access Journals (Sweden)

    García, E.

    2001-08-01

    Full Text Available Among the several techniques available to measure the thermal conductivity/ diffusivity, the laser flash method stands out for its low run time (less than 1 hour per temperature and the reduced sample size required. Nevertheless, the laser flash technique is not very accurate for porous materials due to laser transmission problems and the underestimation of the total sample thickness. The attaching to the porous sample of two thin foils of an opaque and high diffusivity material (Cu can solve these problems. This type of assembly was used to measure diffusivity in a porous alumina material. Data obtained are compared with theoretical models, wich predict effective thermal conductivity of the porous material from the thermal conductivity of the dense material, the amount of porosity and the type of microstructure.

    Entre las distintas técnicas de medida de la conductividad/difusividad térmica de materiales, destaca la técnica del pulso láser por la rapidez de medida de la difusividad térmica y la facilidad de preparación de las muestras. Sin embargo, cuando se mide la difusividad térmica en materiales porosos, aparecen problemas relacionados con la transmisión del haz láser y la subestimacion del espesor de la muestra. En este trabajo, se propone un método para evitar estos problemas que consiste en formar un sistema tricapa Cu/material poroso/Cu. Este método se ha verificado experimentalmente utilizando materiales de alúmina densa y porosa. Los valores experimentales para la alúmina porosa se han explicado a partir de modelos que estiman la conductividad térmica efectiva de un material poroso en función de la conductividad del material denso, la porosidad y el tipo de microestructura.

  5. Enrichment planting without soil treatment

    Energy Technology Data Exchange (ETDEWEB)

    Hagner, Mats

    1998-12-31

    Where enrichment planting had been carried out with either of the two species Picea abies and Pinus contorta, the survival of the planted seedlings was at least as good as after planting in a normal clear cut area treated with soil scarification. This was in spite of the fact that the seedlings were placed shallow in the humus layer without any soil treatment. However, they were sheltered from insects by treatment before planting. Where enrichment planting was carried out with Pinus sylvestris the survival in dense forest was poor, but in open forest the survival was good. The growth of planted seedlings was enhanced by traditional clearing and soil treatment. However, this was for Pinus sylvestris not enough to compensate for the loss of time, 1-2 years, caused by arrangement of soil scarification. The growth of seedlings planted under crown cover was directly related to basal area of retained trees. However, the variation in height growth among individual seedlings was very big, which meant that some seedlings grow well also under a fairly dense forest cover. The pioneer species Pinus sylvestris reacted more strongly to basal area of retained trees than did the shade tolerant species Picea abies. Enrichment planting seems to be a necessary tool for preserving volume productivity, at places where fairly intensive harvest of mature trees has been carried out in stands of ordinary forest type in central Sweden. If double seedlings, with one Picea abies and one Pinus sylvestris, are used, the probability for long term establishment is enhanced 13 refs, 20 figs, 4 tabs

  6. ToNER: A tool for identifying nucleotide enrichment signals in feature-enriched RNA-seq data.

    Directory of Open Access Journals (Sweden)

    Yuttachon Promworn

    Full Text Available Biochemical methods are available for enriching 5' ends of RNAs in prokaryotes, which are employed in the differential RNA-seq (dRNA-seq and the more recent Cappable-seq protocols. Computational methods are needed to locate RNA 5' ends from these data by statistical analysis of the enrichment. Although statistical-based analysis methods have been developed for dRNA-seq, they may not be suitable for Cappable-seq data. The more efficient enrichment method employed in Cappable-seq compared with dRNA-seq could affect data distribution and thus algorithm performance.We present Transformation of Nucleotide Enrichment Ratios (ToNER, a tool for statistical modeling of enrichment from RNA-seq data obtained from enriched and unenriched libraries. The tool calculates nucleotide enrichment scores and determines the global transformation for fitting to the normal distribution using the Box-Cox procedure. From the transformed distribution, sites of significant enrichment are identified. To increase power of detection, meta-analysis across experimental replicates is offered. We tested the tool on Cappable-seq and dRNA-seq data for identifying Escherichia coli transcript 5' ends and compared the results with those from the TSSAR tool, which is designed for analyzing dRNA-seq data. When combining results across Cappable-seq replicates, ToNER detects more known transcript 5' ends than TSSAR. In general, the transcript 5' ends detected by ToNER but not TSSAR occur in regions which cannot be locally modeled by TSSAR.ToNER uses a simple yet robust statistical modeling approach, which can be used for detecting RNA 5'ends from Cappable-seq data, in particular when combining information from experimental replicates. The ToNER tool could potentially be applied for analyzing other RNA-seq datasets in which enrichment for other structural features of RNA is employed. The program is freely available for download at ToNER webpage (http://www4a

  7. ToNER: A tool for identifying nucleotide enrichment signals in feature-enriched RNA-seq data.

    Science.gov (United States)

    Promworn, Yuttachon; Kaewprommal, Pavita; Shaw, Philip J; Intarapanich, Apichart; Tongsima, Sissades; Piriyapongsa, Jittima

    2017-01-01

    Biochemical methods are available for enriching 5' ends of RNAs in prokaryotes, which are employed in the differential RNA-seq (dRNA-seq) and the more recent Cappable-seq protocols. Computational methods are needed to locate RNA 5' ends from these data by statistical analysis of the enrichment. Although statistical-based analysis methods have been developed for dRNA-seq, they may not be suitable for Cappable-seq data. The more efficient enrichment method employed in Cappable-seq compared with dRNA-seq could affect data distribution and thus algorithm performance. We present Transformation of Nucleotide Enrichment Ratios (ToNER), a tool for statistical modeling of enrichment from RNA-seq data obtained from enriched and unenriched libraries. The tool calculates nucleotide enrichment scores and determines the global transformation for fitting to the normal distribution using the Box-Cox procedure. From the transformed distribution, sites of significant enrichment are identified. To increase power of detection, meta-analysis across experimental replicates is offered. We tested the tool on Cappable-seq and dRNA-seq data for identifying Escherichia coli transcript 5' ends and compared the results with those from the TSSAR tool, which is designed for analyzing dRNA-seq data. When combining results across Cappable-seq replicates, ToNER detects more known transcript 5' ends than TSSAR. In general, the transcript 5' ends detected by ToNER but not TSSAR occur in regions which cannot be locally modeled by TSSAR. ToNER uses a simple yet robust statistical modeling approach, which can be used for detecting RNA 5'ends from Cappable-seq data, in particular when combining information from experimental replicates. The ToNER tool could potentially be applied for analyzing other RNA-seq datasets in which enrichment for other structural features of RNA is employed. The program is freely available for download at ToNER webpage (http://www4a.biotec.or.th/GI/tools/toner) and Git

  8. Enriching Students’ Vocabulary Mastery Using Graphic Organizers

    Directory of Open Access Journals (Sweden)

    Syaifudin Latif Darmawan

    2017-04-01

    Full Text Available This action research is carried out to (1 identify whether graphic organizers enrich student’s vocabulary mastery; and (2 to describe the classroom situation when graphic organizers are employed in instructional process of vocabulary. The research is conducted in two cycles from March to May 2016/2017 in the eight years of SMP Muhammadiyah Sekampung, East lampung. The procedure of the research consists of identifying the problem, planning the action, implementing the action, observing the action, and reflecting the result of the research. Qualitative data are collected through interview, observation, questionnaire, and research diary. Quantitative data are collected through test. To analyze qualitative data, the researcher used constant comparative method. It consists of four steps: (1 comparing incidents applicable to each category; (2 Integrating categories and their properties; (3 delimiting the theory; (4 Writing the theory. Meanwhile, to analyze quantitative data, the researcher employed descriptive statistic.    The result of the research shows that using graphic organizers can enrich students’ vocabulary mastery and classroom situation. The improvement on students’ vocabulary included; a the students are able to speak English; b the students are able to understand the meaning of the text as they have a lot of vocabularies. The improvement of the classroom situation; (a students come on time in the class (b students are more motivated to join the class (c Students pay more attention in the instructional process (d students’ participation in responding the questions are high.

  9. [Hydroxylamine conversion by anammox enrichment].

    Science.gov (United States)

    Hu, Anhui; Zheng, Ping; Lu, Huifeng; Ding, Shuang; Wang, Caihua

    2010-04-01

    Hydroxylamine is an important intermediate product of anammox. This study was focused on the characteristics of hydroxylamine and nitrite conversions by anammox enrichment. The changes of nitrogenous substrates and related products with time were measured using batch tests with anammox enrichment as inoculum. Since hydroxylamine didn't react with nitrite in uninoculated control culture, these two compounds were chemically stable. Both of them decreased with time in anammox enrichment inoculated cultures, in which ammonia as intermediate product would be produced and converted with the maximum concentration being 0.338 mg/L. The total nitrogen concentration decreased from 4.694 mmol/L to 0.812 mmol/L with conversion rate 82.7% in the end. When hydroxylamine and nitrite concentrations were about 2.5 mmol/L respectively, the maximum specific sludge conversion rates of hydroxylamine was 0.535 mmol/(gVSS.h), which was 1.81 times bigger than that of ammonia in ammonia reaction system; the maximum specific sludge rate of total nitrogen was slightly higher than that in ammonia reaction system. When hydroxylamine concentration increased to 5.0 mmol/L, the hydroxylamine and nitrite conversion rates promoted by 26.7% and 120.7% respectively; and the maximum ammonia accumulated was 1.810 mmol/L. When nitrite concentration increased to 5.0 mmol/L, the hydroxylamine and nitrite conversion rates promoted by 6.9% and 9.0% respectively; and the maximum ammonia accumulated was 0.795 mmol/L. Anammox enrichment was capable of converting hydroxylamine and nitrite simultaneously and had the higher conversion rate of hydroxylamine than ammonia conversion rate. Hydroxylamine and nitrite conversion rates were less affected by increase in nitrite concentration, but more significantly influenced by increase in hydroxylamine. The maximum ammonia concentration accumulated would rise as the result of increasing both hydroxylamine and nitrite. The result of experiment was consistent with pathway

  10. Boron enrichment in martian clay.

    Directory of Open Access Journals (Sweden)

    James D Stephenson

    Full Text Available We have detected a concentration of boron in martian clay far in excess of that in any previously reported extra-terrestrial object. This enrichment indicates that the chemistry necessary for the formation of ribose, a key component of RNA, could have existed on Mars since the formation of early clay deposits, contemporary to the emergence of life on Earth. Given the greater similarity of Earth and Mars early in their geological history, and the extensive disruption of Earth's earliest mineralogy by plate tectonics, we suggest that the conditions for prebiotic ribose synthesis may be better understood by further Mars exploration.

  11. Integrative set enrichment testing for multiple omics platforms

    Directory of Open Access Journals (Sweden)

    Poisson Laila M

    2011-11-01

    Full Text Available Abstract Background Enrichment testing assesses the overall evidence of differential expression behavior of the elements within a defined set. When we have measured many molecular aspects, e.g. gene expression, metabolites, proteins, it is desirable to assess their differential tendencies jointly across platforms using an integrated set enrichment test. In this work we explore the properties of several methods for performing a combined enrichment test using gene expression and metabolomics as the motivating platforms. Results Using two simulation models we explored the properties of several enrichment methods including two novel methods: the logistic regression 2-degree of freedom Wald test and the 2-dimensional permutation p-value for the sum-of-squared statistics test. In relation to their univariate counterparts we find that the joint tests can improve our ability to detect results that are marginal univariately. We also find that joint tests improve the ranking of associated pathways compared to their univariate counterparts. However, there is a risk of Type I error inflation with some methods and self-contained methods lose specificity when the sets are not representative of underlying association. Conclusions In this work we show that consideration of data from multiple platforms, in conjunction with summarization via a priori pathway information, leads to increased power in detection of genomic associations with phenotypes.

  12. Method for selective recovery of PET-usable quantities of [{sup 18}F] fluoride and [{sup 13}N] nitrate/nitrite from a single irradiation of low-enriched [{sup 18}O] water

    Science.gov (United States)

    Ferrieri, R.A.; Schlyer, D.J.; Shea, C.

    1995-06-13

    A process for simultaneously producing PET-usable quantities of [{sup 13}N]NH{sub 3} and [{sup 18}F]F{sup {minus}} for radiotracer synthesis is disclosed. The process includes producing [{sup 13}N]NO{sub 2}{sup {minus}}/NO{sub 3}{sup {minus}}and [{sup 18}F]F{sup {minus}} simultaneously by exposing a low-enriched (20%-30%) [{sup 18}O]H{sub 2}O target to proton irradiation, sequentially isolating the [{sup 13}N]NO{sub 2}{sup {minus}}/NO{sub 3}{sup {minus}} and [{sup 18}F]F{sup {minus}} from the [{sup 18}O]H{sub 2}O target, and reducing the [{sup 13}N]NO{sub 2}{sup {minus}}/NO{sub 3}{sup {minus}} to [{sup 13}N]NH{sub 3}. The [{sup 13}N]NH{sub 3} and [{sup 18}F]F{sup {minus}} products are then conveyed to a laboratory for radiotracer applications. The process employs an anion exchange resin for isolation of the isotopes from the [{sup 18}O]H{sub 2}O, and sequential elution of [{sup 13}N]NO{sub 2}{sup {minus}}/NO{sub 3}{sup {minus}} and [{sup 18}F]F{sup {minus}} fractions. Also the apparatus is disclosed for simultaneously producing PET-usable quantities of [{sup 13}N]NH{sub 3} and [{sup 18}F]F{sup {minus}} from a single irradiation of a single low-enriched [{sup 18}O]H{sub 2}O target. 2 figs.

  13. Medidas de bioseguridad adoptadas en el manejo con materiales biológicos en Laboratorios Liorad Biosafety measures adopted in Liorad Laboratories for handling biological materials

    Directory of Open Access Journals (Sweden)

    Nancy Burguet Lago

    2013-03-01

    Full Text Available Introducción: el trabajo con microorganismos puede conllevar a riesgos tanto para el personal que trabaja con los mismos como para el medio ambiente. La existencia de laboratorios de seguridad biológica y la implementación de medidas en la manipulación de los agentes biológicos minimizan el riesgo. Objetivo: evaluar las medidas de bioseguridad adoptadas en el manejo con materiales biológicos en Laboratorios Liorad. Métodos: empleo de una lista de chequeo y análisis de los resultados a través de una Matriz DAFO para valorar si el diseño de la instalación cumple con la bioseguridad. Además establecer un sistema documental para la manipulación de microorganismos y la confección de un plan de capacitación para el personal que trabaja en el laboratorio de control microbiológico. Resultados: la lista de chequeo permitió identificar como principal debilidad el no disponer de un doble pasillo para el traslado del material limpio y sucio. Como fortalezas, cumplir con las prácticas y procesamientos adecuados y el contar con equipos de seguridad biológica. El sistema documental incorporó a los procedimientos establecidos para la manipulación, un acápite referido a la «Peligrosidad y Medidas de Seguridad». El programa de capacitación desarrollado permitió proveer conocimientos específicos referidos a esta temática. Conclusión: las medidas adoptadas en el laboratorio permiten plantear que de manera general se cumplen los requisitos establecidos en materia de Bioseguridad para el trabajo con microorganismos.Introduction: working with microorganisms can lead to risks for both the staff at work and the environment. The existence of biosafety labs and implementation of measures in the handling of biological agents minimize the risk. Objetive: to evaluate biosecurity measures taken in handling biological materials at Liorad Laboratories. Methods: using a checklist and analysis of results through a SWOT Matrix to assess whether the

  14. Reflexiones sobre el problema del analfabetismo y propuesta de algunas medidas pedagógicas para combatirlo

    Directory of Open Access Journals (Sweden)

    Maria José NAVARRO PERALES

    2009-11-01

    Full Text Available El analfabetismo es la consecuencia de la desigualdad e injusticia social. Combatirlo es una labor igualmente social. Las medidas deben ser tomadas por los gobiernos a través de instituciones de educación formal y no formal. La prevención y desaparición del analfabetismo, en todas sus modalidades y grados, debe realizarse desde el punto de vista del analfabeto y no contra él.

  15. Medida da visão em candelas: description of a psychophysical technique to quantify luminous intensity

    Directory of Open Access Journals (Sweden)

    Airton Leite Kronbauer

    2011-04-01

    Full Text Available OBJETIVO: Desenvolver um método e um dispositivo para quantificar a visão em candela (cd. Os estudos de medida da visão são importantes para todas as ciências visuais. MÉTODOS: É um estudo teórico e experimental. Foram descritos os detalhes do método psicofísico e da calibração do dispositivo. Foram realizados testes preliminares em voluntários. RESULTADOS: É um teste psicofísico simples e com resultado expresso em unidades do sistema internacional de medidas. Com a descrição técnica será possível reproduzir o experimento em outros centros de pesquisa. CONCLUSÃO: Os resultados aferidos em intensidade luminosa (cd são uma opção para estudo visual. Esses resultados possibilitarão extrapolar medidas para modelos matemáticos e para simular efeitos individuais com dados aberrométricos.

  16. Nivel de conocimiento y aplicación de las medidas de bioseguridad del personal de enfermería

    Directory of Open Access Journals (Sweden)

    Luz Marina Bautista-Rodríguez

    2013-12-01

    Full Text Available Objetivo. Identificar el nivel de conocimiento y aplicación de las medidas de bioseguridad que tiene el personal de Enfermería. Materiales y Métodos. Se realizó investigación cuantitativa, de tipo descriptivo transversal, con una muestra de 96 personas pertenecientes. La información se recolectó a través de una encuesta y una lista de chequeo, la cual se analizó por medio de tabulaciones y representaciones gráficas. Resultados. El personal de Enfermería de la Clínica San José tiene un conocimiento regular en un 66% frente a las medidas de bioseguridad y un 70% de aplicación deficiente frente a estas. Conclusión. Se identificó que las principales medidas de bioseguridad, como métodos de barrera, eliminación adecuada del material contaminado, manejo adecuado de los elementos cortopunzante, lavado de manos no están siendo aplicadas correctamente por el personal de Enfermería de la institución, convirtiéndose estas situaciones en un factor de riesgo para el presentar un accidente laboral esta población.

  17. Medidas de protección del menor en los casos de violencia de género

    Directory of Open Access Journals (Sweden)

    Francisca Ramón Fernández

    2013-04-01

    Full Text Available En el presente trabajo nos proponemos realizar un análisis de los distintos preceptos que contemplan las medidas de protección del menor en los casos de violencia de género, tanto en la legislación internacional, estatal y autonómica. El menor como sujeto, directo o indirecto, de la violencia sobre la mujer es protegido a través de una serie de medidas y reconocimiento de derechos, que es más amplio en una legislación que en otra de las estudiadas. Sería conveniente una uniformidad sobre esa protección al menor, ya que en la mayoría de las veces sufre esa violencia en el entorno familiar. La reflexión del análisis de los preceptos nos indica que es el interés superior del menor el que tiene que prevalecer en todas las situaciones, y que, en muchas ocasiones, la legislación no dota de suficientes medidas de protección al menor que se encuentra en una situación de violencia doméstica.

  18. Preferencias de estados de salud y medidas de utilidad = Preferences of states of health and measurements of utility

    Directory of Open Access Journals (Sweden)

    Yepes-Nuñez, Juan José

    2011-12-01

    Full Text Available La medición de los valores del estado de salud, preferencias y utilidad calcula el valor o la conveniencia de un estado de salud contra un instrumento externo; son medidas genéricas de la calidad de vida relacionada con la salud (CVRS y resumen el valor de esta en un solo número entre 0 (cero y 1 (uno. Las medidas de preferencias de estados de salud y de utilidad difieren de las medidas generales y específicas obtenidas mediante cuestionarios de CVRS en áreas importantes. Primero, calculan las predilecciones de los individuos por alternativas. Segundo, proveen un resultado completo de la CVRS. Tercero, ofrecen una unidad común de análisis. Existen dos métodos para la medición de las utilidades, uno directo y otro indirecto, que se conocen también como utilidades multiatributo o sistemas de clasificación de estados de salud. El proceso directo de medición consiste en un agregado de descripciones que un entrevistador presenta a los encuestados. Para las mediciones indirectas se aplican solamente cuestionarios. El objetivo principal de este artículo es describir los principales métodos de valoración de utilidades, con el fin de dar a conocer los desenlaces que actualmente se utilizan tanto en estudios de CVRS como en evaluaciones económicas.

  19. Principal Angle Enrichment Analysis (PAEA): Dimensionally Reduced Multivariate Gene Set Enrichment Analysis Tool.

    Science.gov (United States)

    Clark, Neil R; Szymkiewicz, Maciej; Wang, Zichen; Monteiro, Caroline D; Jones, Matthew R; Ma'ayan, Avi

    2015-11-01

    Gene set analysis of differential expression, which identifies collectively differentially expressed gene sets, has become an important tool for biology. The power of this approach lies in its reduction of the dimensionality of the statistical problem and its incorporation of biological interpretation by construction. Many approaches to gene set analysis have been proposed, but benchmarking their performance in the setting of real biological data is difficult due to the lack of a gold standard. In a previously published work we proposed a geometrical approach to differential expression which performed highly in benchmarking tests and compared well to the most popular methods of differential gene expression. As reported, this approach has a natural extension to gene set analysis which we call Principal Angle Enrichment Analysis (PAEA). PAEA employs dimensionality reduction and a multivariate approach for gene set enrichment analysis. However, the performance of this method has not been assessed nor its implementation as a web-based tool. Here we describe new benchmarking protocols for gene set analysis methods and find that PAEA performs highly. The PAEA method is implemented as a user-friendly web-based tool, which contains 70 gene set libraries and is freely available to the community.

  20. Tritium enrichment in aqueous phase by catalytic isotopic exchange

    International Nuclear Information System (INIS)

    Schindewolf, U.

    1983-01-01

    At normal pressure, the most promising enrichment method appears to be an inverse current exchanger system using electrolysis at the bottom of the inverse current column apparatus and recombination in an oxyhydrogen gas flame at the top of the column apparatus. A hydrophobic catalyst is used which is made of a pulverized mix of platinized activated carbon and PTFE. (DG) [de

  1. Titanium dioxide enrichment of sialic acid-containing glycopeptides

    DEFF Research Database (Denmark)

    Palmisano, Giuseppe; Lendal, Sara E; Larsen, Martin Røssel

    2011-01-01

    the glycosylation site of N-linked sialylated glycoproteins. The method relies on the specificity of titanium dioxide affinity chromatography to isolate sialic acid-containing glycopeptides. After enzymatic release of the glycans, the enriched sialylated glycopeptides are analyzed by mass spectrometry...

  2. Genetic engineering of syringyl-enriched lignin in plants

    Science.gov (United States)

    Chiang, Vincent Lee; Li, Laigeng

    2004-11-02

    The present invention relates to a novel DNA sequence, which encodes a previously unidentified lignin biosynthetic pathway enzyme, sinapyl alcohol dehydrogenase (SAD) that regulates the biosynthesis of syringyl lignin in plants. Also provided are methods for incorporating this novel SAD gene sequence or substantially similar sequences into a plant genome for genetic engineering of syringyl-enriched lignin in plants.

  3. Isotopic enrichment of 15N by ionic exchange chromatography

    International Nuclear Information System (INIS)

    Trivelin, P.C.O.

    1979-01-01

    The present paper presents some studies on production of 15 N-enriched ammonium sulphate with 5% atoms by ionic exchange chromatography method. Two systems are described of columns of resin, where experiments were conducted by eluition of NH 4 + bands with sodium hydroxide solution. Analyses were made of the cost of production of 15 N-enriched ammonium sulphate 5% atoms and, based on the experiments developed, a cost was obtained which was compatible with the international price of the product. The isotopic analyses of nitrogen were made by mass spectrometry. (Author) [pt

  4. Oxygen enriched air using membrane for palm oil wastewater treatment

    Directory of Open Access Journals (Sweden)

    Ramlah Mohd Tajuddin

    2002-11-01

    Full Text Available A research aimed to explore new method of aeration using oxygen enriched air performance on BOD reduction of palm oil wastewater was conducted. The oxygen enriched air was obtained from an Oxygen Enriched System (OES developed using asymmetric polysulfone hollow fiber membrane with composition consisting of PSF: 22%, DMAc: 31.8%, THF: 31.8%, EtOH: 14.4%. Palm oil wastewater samples were taken from facultative pond effluent. These samples were tested for its initial biochemical oxygen demand (BOD, total suspended solids (TSS, pH, conductivity, turbidity, dissolved oxygen (DO, suspended solids (SS, and total dissolved solids (TDS before being subjected to two modes of aeration system, that is diffused air and oxygen enriched air. These water quality concentrations were tested for every 20 minutes for two-hour period during the aeration process. Results of BOD, TSS, pH, conductivity, DO, SS and TDS concentrations against time of samples from the two modes of aeration were then compared. It was found that DO concentration achieved in oxygen enriched air aeration was better than aeration using diffused air system. Aeration using OES improve the DO concentration in the wastewater and thus improve the BOD reduction and also influence other physical characteristics of wastewater. This phenomenon indicates the advantage of using air with higher oxygen concentration for wastewater aeration instead of diffused air system.

  5. Feasibility of Low Enriched Uranium Fuel for Space Nuclear Propulsion

    Energy Technology Data Exchange (ETDEWEB)

    Venneri, Paolo; Kim, Yonghee [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of)

    2013-05-15

    The purpose of this initial study is to create a baseline with which to perform further analysis and to build a solid understanding of the neutronic characteristics of a solid core for the nuclear thermal rocket. Once consistency with work done at Idaho National Laboratory (INL) is established, this paper will provide a study of other fuel types, such as low and medium-enriched uranium fuels. This paper will examine how the implementation of each fuel type affects the multiplication factor of the reactor, and will then explore different possibilities for alterations needed to accommodate their successful usage. The reactor core analysis was done using the MCNP5 code. While this study has not shown that the SNRE can be easily retrofitted for low-enriched U fuel, it has made a detailed study of the SNRE, and identified the difficulties of the implementation of low-enriched fuels in small nuclear rockets. These difficulties are the need for additional moderation and fuel mass in order to achieve a critical mass. Neither of these is insurmountable. Future work includes finding the best method by which to increase the internal moderation of the reactor balanced with appropriate sizing to prevent neutron leakage. Both of these are currently being studied. This paper will present a study of the Small Nuclear Rocket Engine (SNRE) and the feasibility of using low enriched Uranium (LEU) instead of the traditional high enriched Uranium (HEU) fuels.

  6. DNA enrichment approaches to identify unauthorized genetically modified organisms (GMOs).

    Science.gov (United States)

    Arulandhu, Alfred J; van Dijk, Jeroen P; Dobnik, David; Holst-Jensen, Arne; Shi, Jianxin; Zel, Jana; Kok, Esther J

    2016-07-01

    With the increased global production of different genetically modified (GM) plant varieties, chances increase that unauthorized GM organisms (UGMOs) may enter the food chain. At the same time, the detection of UGMOs is a challenging task because of the limited sequence information that will generally be available. PCR-based methods are available to detect and quantify known UGMOs in specific cases. If this approach is not feasible, DNA enrichment of the unknown adjacent sequences of known GMO elements is one way to detect the presence of UGMOs in a food or feed product. These enrichment approaches are also known as chromosome walking or gene walking (GW). In recent years, enrichment approaches have been coupled with next generation sequencing (NGS) analysis and implemented in, amongst others, the medical and microbiological fields. The present review will provide an overview of these approaches and an evaluation of their applicability in the identification of UGMOs in complex food or feed samples.

  7. Fusão INS/GNSS com auxílio de medidas de baseline e ângulo GNSS

    OpenAIRE

    Priscylla Angélica da Silva Oliveira

    2014-01-01

    Este trabalho investiga sistemas de navegação INS/GNSS auxiliado por medidas de baseline e ângulo GNSS. Os dados gerados para as simulações foram os dados IMU, dados GNSS e informações de baseline e ângulo GNSS. Estas informações, determinadas a partir de um sistema multiantenas para o posicionamento simples e relativo e no método direto, respectivamente, foram utilizadas como medidas auxiliares para a fusão INS/GNSS. Foi estudada a influência dessas medidas auxiliares nas estimativas de atit...

  8. Diferencia en la medida centrada y descentrada del espesor y volumen retiniano con Tomografía de Coherencia Óptica (OCT 3D)

    OpenAIRE

    Carrero Peñaranda, Rocio

    2013-01-01

    Se realizaron diferentes medidas centradas y descentradas del espesor y volumen retiniano con OCT para determinar si un ligero descentramiento en la medida puede ocasionar una medida errónea del espesor macular y, en consecuencia, una mala decisión por parte del oftalmólogo a la hora de decidir un tratamiento en pacientes con edema macular. Máster en Física de los sistemas de diagnóstico, tratamiento y protección en Ciencias de la Salud

  9. Crop responses to CO2 enrichment

    International Nuclear Information System (INIS)

    Rogers, H.H.; Dahlman, R.C.

    1993-01-01

    Carbon dioxide is rising in the global atmosphere, and this increase can be expected to continue into the foreseeable future. This compound is an essential input to plant life. Crop function is affected across all scales from biochemical to agroecosystem. An array of methods (leaf cuvettes, field chambers, free-air release systems) are available for experimental studies of CO 2 effects. Carbon dioxide enrichment of the air in which crops grow usually stimulates their growth and yield. Plant structure and physiology are markedly altered. Interactions between CO 2 and environmental factors that influence plants are known to occur. Implications for crop growth and yield are enormous. Strategies designed to assure future global food security must include a consideration of crop responses to elevated atmospheric CO 2 . 137 refs., 4 figs., 4 tabs

  10. An optimal sequence of the reload charge fuel enrichment to a reactor

    International Nuclear Information System (INIS)

    Sato, S.

    1975-01-01

    An optimal sequence of enrichment of the reload charge of a three regions PWR during its life has been determined by dynamic programming. The state of the reactor is specified by the burnup of the fuel in the three regions and their initial enrichments. Constraints were imposed on the power peaking factor, the maximum burnup, the length of each stage between refueling and the total life of the reactor. 'Central-scatter loading' was assumed at each reloading. The two group diffusion equations were solved by the modal method for the static calculations of the reactor. Otimization of enrichment of the reload charge was performed under several hypotheses on the variation of the costs of uranium, costs of enrichment and the plant factor during the reactor life. It was observed that the optimum enrichment of the reload fuel is influenced more by the cost of enrichment rather than plant factor or cost of uranium. (Author) [pt

  11. Study of Fuel Rods Axial Enrichment Distribution Effect on the Neutronic Parameters of the Reactor Core

    International Nuclear Information System (INIS)

    Pazirandeh, A.; Nasiri, S. H.

    2012-01-01

    Optimization of the fuel burn up is an important issue in nuclear reactor fuel management and technology. Radial enrichment distribution in the reactor core is a conventional method and axial enrichment is constant along the fuel rod. In this article, the effects of axial enrichment distribution variation on neutronic parameters of PWR core are studied. The axial length of the core is divided into ten sections, considering axial enrichment variation and leaving the existing radial enrichment distribution intact. This study shows that the radial and axial power peaking factors are decreased as compared with the typical conventional core. In addition, the first core lifetime lasts 30 days longer than normal PWR core. Moreover, at the same time boric acid density is 0.2 g/kg at the beginning of the cycle. The flux shape is also flat at the beginning of the cycle for the proposed configuration of the axially enrichment distribution.

  12. Hybrid Enrichment Verification Array: Module Characterization Studies

    Energy Technology Data Exchange (ETDEWEB)

    Zalavadia, Mital A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Smith, Leon E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); McDonald, Benjamin S. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Kulisek, Jonathan A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mace, Emily K. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Deshmukh, Nikhil S. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-03-01

    The work presented in this report is focused on the characterization and refinement of the Hybrid Enrichment Verification Array (HEVA) approach, which combines the traditional 186-keV 235U signature with high-energy prompt gamma rays from neutron capture in the detector and surrounding collimator material, to determine the relative enrichment and 235U mass of the cylinder. The design of the HEVA modules (hardware and software) deployed in the current field trial builds on over seven years of study and evolution by PNNL, and consists of a ø3''×3'' NaI(Tl) scintillator coupled to an Osprey digital multi-channel analyzer tube base from Canberra. The core of the HEVA methodology, the high-energy prompt gamma-ray signature, serves as an indirect method for the measurement of total neutron emission from the cylinder. A method for measuring the intrinsic efficiency of this “non-traditional” neutron signature and the results from a benchmark experiment are presented. Also discussed are potential perturbing effects on the non-traditional signature, including short-lived activation of materials in the HEVA module. Modeling and empirical results are presented to demonstrate that such effects are expected to be negligible for the envisioned implementation scenario. In comparison to previous versions, the new design boosts the high-energy prompt gamma-ray signature, provides more flexible and effective collimation, and improves count-rate management via commercially available pulse-processing electronics with a special modification prompted by PNNL.

  13. Medidas de frecuencia, asociación e impacto en investigación aplicada

    Directory of Open Access Journals (Sweden)

    J.A. Mirón Canelo

    2008-06-01

    Full Text Available En la Sociedad actual del conocimiento y la información es preciso disponer de herramientas básicas para medir los fenómenos epidemiológicos como la enfermedad, la incapacidad o la siniestralidad laboral. Las formas de medir son instrumentos que se deben conocer y aplicar para planificar y tomar decisiones en Salud Pública y en Salud Laboral. El objetivo de este artículo es dar a conocer las principales medidas de frecuencia, asociación e impacto utilizadas en investigación aplicada, clínica o de Salud Pública para tratar de medir, valorar y estimar la importancia de los problemas de salud y enfermedades de relacionadas con el trabajo, de los factores de riesgo ocupacionales y otros eventos relacionados con la seguridad e higiene en el trabajo y, en definitiva, con la Salud de los trabajadores. Se describen los conceptos teóricos de las formas de medir en epidemiología, su interpretación y aplicación práctica de los indicadores básicos utilizados en la práctica habitual de los profesionales de las Ciencias de la Salud. Su utilidad fundamental es poder disponer de una información objetiva, fiable y precisa que permita tomar decisiones adecuadas y pertinentes en relación con la prevención, seguridad laboral, atención y rehabilitación de los trabajadores.In the society´current knowledge and information is necessary to have basic tools to measure the epidemic phenomena such as illness, disability or workplace accidents. The ways of measuring are instruments that they must know and apply to plan and take decisions on Public Health and Labour/Occupational Health. The aim of this article is to inform about the most important measures of frequency, association and impact used in applied research, clinical or Public Health to try to measure, to value and estimate the importance of health problems and diseases related work. The occupational risk factors and other events related to safety and healthy working conditions and ultimately

  14. Ciberactivismo: conceptualización, hipótesis y medida

    Directory of Open Access Journals (Sweden)

    Fernández Prados, Juan Sebastián

    2012-08-01

    Full Text Available Cyberactivism has emerged as a transposition of unconventional political action to the context of information and communication technologies. This phenomenon raises at least three questions. The first is the definition, delineation and classification of the concept. The second is that of the theoretical perspectives from which to answer questions such as: does the emergence of ICTs in the public sphere enhance civic culture or not? This question led to Pippa Norris to distinguish between mobilization theories and theories of reinforcement. Finally, there is a need to design tools and studies with which to attempt to evaluate and measure the phenomenon, as proposed by the biannual study entitled The Internet in Britain and in the work of the University of Southern California through the Center for the Digital Future. Following this review, we develop a new definition of cyberactivism and propose some instrument to measure the phenomenon.

    La emergencia del ciberactivismo como una traslación de la acción política no convencional en el contexto de las tecnologías de la información y la comunicación plantea al menos tres cuestiones. La definición, delimitación y clasificación del mismo concepto. El segundo aspecto que suscita el ciberactivismo es de carácter teórico al plantear las siguientes perspectivas antitéticas: ¿Provoca o no la aparición de las TIC en la esfera política un aumento de la cultura cívica? Pregunta que lleva a Pippa Norris a distinguir entre las teorías de la movilización y las teorías del refuerzo. Finalmente, surge la necesidad de diseñar instrumentos y estudios que procuran en parte una evaluación y medida del fenómeno del ciberactivismo tal y como lo lleva a cabo el estudio bianual The Internet in Britain o los trabajos de la Universidad del Sur de California a través del Center for the Digital Future. Tras esta revisión, desarrollaremos propuestas conceptuales y metodológicas para medir

  15. Gasket for uranium enrichment plant

    Energy Technology Data Exchange (ETDEWEB)

    Kishi, S; Aiyoshi, H

    1977-02-08

    A gasket to be inserted between flange joints in the equipments and pipe lines of an uranium enrichment plant having neither permeability nor adsorptivity to water while maintaining mechanical, physical and chemical properties of an elastomer gasket is described. A gasket made of an elastomeric material such as a polymer is integratedly formed at its surface with anti-slip projections. The gasket is further surrounded at its upper and lower peripheral sides, as well as outer circumferential portion with a U-sectioned cover (enclosure) made of fluoro-plastics. In this arrangement, the gasket main body shows a gas-tightness for uranium hexafluoride gas and the cover exhibits a gas-tightness for other component gases such as moisture to thereby prevent degradation of the gasket due to absorption and permeation of the moisture.

  16. Uranium enrichment in South Africa

    International Nuclear Information System (INIS)

    Roux, A.J.A.; Grant, W.L.

    1976-01-01

    It is stated that the South African process is of an aerodynamic type, the separating element being in effect a high performance stationary-walled centrifuge using UF 6 in hydrogen as process fluid. Some details of the very low uranium inventory and high separation factor achievable are given. A new cascade technique is described, based on the principle that an axial flow compressor can simultaneously transmit several streams of different isotopic composition without there being significant mixing between them. The research and development programme is discussed. It is expected that an enrichment plant of 5000 t/a SW capacity, with provision for expansion up to 10,000 t/a SW capacity, will come into operation by 1984. (U.K.)

  17. Stable isotope enrichment: Current and future potential

    International Nuclear Information System (INIS)

    Tracy, J.G.; Aaron, W.S.

    1992-01-01

    Oak Ridge National Laboratory (ORNL) operates the Isotope Enrichment Facility for the purpose of providing enriched stable isotopes, selected radioactive isotopes (including the actinides), and isotope-related materials and services for use in various research applications. ORNL is responsible for isotope enrichment and the distribution of approximately 225 nongaseous stable isotopes from 50 multi-isotopic elements. Many enriched isotope products are of prime importance in the fabrication of nuclear targets and the subsequent production of special radionuclides. State-of-the-art techniques to achieve special isotopic, chemical, and physical requirements are performed at ORNL This report describes the status and capabilities of the Isotope Enrichment Facility and the Isotope Research Materials Laboratory as well as emphasizing potential advancements in enrichment capabilities

  18. Considering the post-1995 enrichment market

    International Nuclear Information System (INIS)

    Gunter, L.

    1994-01-01

    World demand for uranium enrichment services is likely to grow only a little over the next decade, from the current 28 million separative work units (SWU) per year to 33 MSWU per year. Much of the growth will come from Asia where nuclear generating capacity is still increasing. The current situation of the primary enrichment companies is summarized. The primary Western suppliers, Cogema, United States Enrichment Corporation and Urenco, are competing for increased market share in the USA, Europe and Asia as utilities purchase their post-1995 requirements. Entry of the Russian enrichment company, Tenex, into Western markets has been limited by trade restrictions. As a consequence of disarmament, blended weapons material has resulted in a surplus of low-enriched uranium. Together with over-capacity amongst the primary enrichers this has led to an expectation that reduced prices will be negotiable in the medium term. (3 figures). (UK)

  19. Noble gas enrichment studies at JET

    International Nuclear Information System (INIS)

    Groth, M.; Andrew, P.; Fundamenski, W.; Guo, H.Y.; Hillis, D.L.; Hogan, J.T.; Horton, L.D.; Matthews, G.F.; Meigs, A.G.; Morgan, P.M.; Stamp, M.F.; Hellermann, M. von

    2001-01-01

    Adequate helium exhaust has been achieved in reactor-relevant ELMy H-mode plasmas in JET performed in the MKII AP and MKII GB divertor geometry. The divertor-characteristic quantities of noble gas compression and enrichment have been experimentally inferred from Charge Exchange Recombination Spectroscopy measurements in the core plasma, and from spectroscopic analysis of a Penning gauge discharge in the exhaust gas. The retention of helium was found to be satisfactory for a next-step device, with enrichment factors exceeding 0.1. The helium enrichment decreases with increasing core plasma density, while the neon enrichment has the opposite behaviour. Analytic and numerical analyses of these plasmas using the divertor impurity code package DIVIMP/NIMBUS support the explanation that the enrichment of noble gases depends significantly on the penetration depth of the impurity neutrals with respect to the fuel atoms. Changes of the divertor plasma configuration and divertor geometry have no effect on the enrichment

  20. Stable isotope enrichment - current and future potential

    International Nuclear Information System (INIS)

    Tracy, J.G.; Aaron, W.S.

    1993-01-01

    Oak Ridge National Laboratory (ORNL) operates the Isotope Enrichment Facility for the purpose of providing enriched stable isotopes, selected radioactive isotopes (including the actinides), and isotope-related materials and services for use in various research applications. ORNL is responsible for isotope enrichment and the distribution of approximately 225 nongaseous stable isotopes from 50 multi-isotopic elements. Many enriched isotope products are of prime importance in the fabrication of nuclear targets and the subsequent production of special radionuclides. State-of-the-art techniques to achieve special isotopic, chemical, and physical requirements are performed at ORNL. This report describes the status and capabilities of the Isotope Enrichment Facility and the Isotope Research Materials Laboratory as well as emphasizing potential advancements in enrichment capabilities. (orig.)

  1. Measurement of the enrichment of uranium-hexafluoride gas in product pipes in the centrifuge enrichment plant at Almelo

    International Nuclear Information System (INIS)

    Packer, T.W.; Lees, E.W.; Aaldijk, J.K.; Harry, R.J.S.

    1987-09-01

    One of the objectives of safeguarding centrifuge enrichment plants is to apply non-destructive measurements inside the cascade area to confirm that the enrichment level is in the low enriched uranium range. Research in the UK and USA has developed a NDA instrument which can confirm the presence of low enriched uranium on a rapid go/no go basis in cascade header pipework of their centrifuge enrichment plants. The instrument is based on a gamma spectroscopic measurement coupled with an X-ray fluorescence analysis. This report gives the results of measurements carried out at Almelo by the UKAEA Harwell, ECN Petten and KFA Juelich to determine if these techniques could be employed at Almelo and Gronau. The energy dispersive X-ray fluorescence analysis has been applied to determine the total mass of uranium in the gas phase, and the deposit correction technique and the two geometry technique have been applied at Almelo to correct the measured gamma intensities for those emitted by the deposit. After an executive summary the report discusses the principles of the two correction methods. A short description of the equipment precedes the presentation of the results of the measurements and the discussion. After the conclusions the report contains two appendices which contain the derivation of the formulae for the deposit correction technique and a discussion of the systematic errors of this technique. 8 figs.; 11 refs.; 6 tables

  2. Turkey's regulatory plans for high enriched to low enriched conversion of TR-2 reactor core

    International Nuclear Information System (INIS)

    Guelol Oezdere, Oya

    2003-01-01

    Turkey is a developing country and has three nuclear facilities two of which are research reactors and one pilot fuel production plant. One of the two research reactors is TR-2 which is located in Cekmece site in Istanbul. TR-2 Reactor's core is composed of both high enriched and low enriched fuel and from high enriched to low enriched core conversion project will take place in year 2005. This paper presents the plans for drafting regulations on the safety analysis report updates for high enriched to low enriched core conversion of TR-2 reactor, the present regulatory structure of Turkey and licensing activities of nuclear facilities. (author)

  3. EURODIF: the uranium enrichment by gaseous diffusion

    International Nuclear Information System (INIS)

    Rougeau, J.P.

    1981-01-01

    During the seventies the nuclear power programme had an extremely rapid growth rate which entailed to increase the world uranium enrichment capacity. EURODIF is the largest undertaking in this field. This multinational joint venture built and now operates and enrichment plant using the gaseous diffusion process at Tricastin (France). This plant is delivering low enriched uranium since two years and has contracted about 110 million SWU's till 1990. Description, current activity and prospects are given in the paper. (Author) [pt

  4. Process for the production of protein enriched fractions from vegetable materials

    NARCIS (Netherlands)

    Dijkink, B.H.; Willemsen, J.H.A.

    2006-01-01

    The present invention provides a method for the production of a protein enriched fraction and a fibre enriched fraction from a vegetable material, wherein the vegetable material comprises a total fat content of 0.1 to 22.0 % by dry weight of the total vegetable material and a total starch content of

  5. Hyb-Seq: combining target enrichment and genome skimming for plant phylogenomics

    Science.gov (United States)

    Kevin Weitemier; Shannon C.K. Straub; Richard C. Cronn; Mark Fishbein; Roswitha Schmickl; Angela McDonnell; Aaron. Liston

    2014-01-01

    • Premise of the study: Hyb-Seq, the combination of target enrichment and genome skimming, allows simultaneous data collection for low-copy nuclear genes and high-copy genomic targets for plant systematics and evolution studies. • Methods and Results: Genome and transcriptome assemblies for milkweed ( Asclepias syriaca ) were used to design enrichment probes for 3385...

  6. Elucidation of time-dependent systems biology cell response patterns with time course network enrichment

    DEFF Research Database (Denmark)

    Wiwie, Christian; Rauch, Alexander; Haakonsson, Anders

    2018-01-01

    , no methods exist to integrate time series data with networks, thus preventing the identification of time-dependent systems biology responses. We close this gap with Time Course Network Enrichment (TiCoNE). It combines a new kind of human-augmented clustering with a novel approach to network enrichment...

  7. Protein biomarker enrichment by biomarker antibody complex elution for immunoassay biosensing

    NARCIS (Netherlands)

    Sabatté, G.S.; Feitsma, H.; Evers, T.H.; Prins, M.W.J.

    2011-01-01

    It is very challenging to perform sample enrichment for protein biomarkers because proteins can easily change conformation and denature. In this paper we demonstrate protein enrichment suited for high-sensitivity integrated immuno-biosensing. The method enhances the concentration of the biomarkers

  8. Expansion of U.S. uranium enrichment capacity. Final environmental statement

    International Nuclear Information System (INIS)

    1976-04-01

    Reasonably foreseeable environmental, social, economic, and technological costs and benefits of postulated expansion of U. S. enrichment capacity through the year 2000 and reasonably available alternatives to such expansion are described. Both the gas centrifuge and gaseous diffusion methods for the enrichment of uranium are considered in this impact assessment

  9. Simplex Optimization of Headspace-Enrichment Conditions of Residual Petroleum Distillates Used by Arsonists

    Science.gov (United States)

    Warnke, Molly M.; Erickson, Angela E.; Smith, Eugene T.

    2005-01-01

    A forensic project is described that is suitable for an undergraduate instrumental methods lab. Accelerants commonly used by arsonists are analyzed by static headspace enrichment followed by gas chromatography. The conditions used for headspace enrichment (e.g., time and temperature) are known to influence the distribution of hydrocarbons…

  10. The future cost of uranium enrichment

    International Nuclear Information System (INIS)

    Pouris, A.

    1986-01-01

    The cost of uranium enrichment is the most important factor determining the fuel cost of nuclear energy. This paper attempts to forecast the future direction of the price of separative work by examining the forces that determine it. It is argued that the interplay among the characteristics of enrichment technologies, the structure of the international market, and the balance of supply and demand determine the enrichment price. The analysis indicates that all forces point towards a price much lower than the current one. It is predicted that, depending on the technological advances, the price of separative work unit for uranium enrichment will range between $40 and $90 by the year 2000. (author)

  11. Consciência metatextual: evidências de validade para instrumento de medida Consciencia metatextual: evidencias de validez para instrumento de medida Metatextual awareness: evidence of validity for measuring instrument

    Directory of Open Access Journals (Sweden)

    Acácia Aparecida Angeli dos Santos

    2012-08-01

    Full Text Available O objetivo estabelecido nesta pesquisa descritivo-correlacional foi a averiguação da consciência metatextual por meio de um questionário especialmente elaborado para este fim, analisando sua validade pela relação com medidas de compreensão de leitura e o critério de diferenciação do nível escolar. Participaram 315 alunos do 3º ao 5º ano do ensino fundamental, de escolas públicas, cujas idades variaram de 8 a 13 anos, sendo 169 meninos. Os escores obtidos com a medida de avaliação da consciência metatextual (QACM foram convergentes com a pontuação em compreensão de leitura e o instrumento mostrou-se sensível para captar o progresso das crianças em identificar os gêneros textuais com o avanço da escolaridade. Dessa forma, o estudo forneceu evidências de validade tanto no que se refere à convergência de construtos, identificada com base nas medidas relacionadas, como de critério, distinguindo os alunos pelo seu nível de escolaridade.El objetivo establecido en esta investigación descriptivo-correlacional fue averiguar la consciencia metatextual a través de un cuestionario especialmente diseñado para este fin, analizando su validez por la relación con medidas de comprensión de lectura y el criterio de diferenciación por nivel escolar. Participaron 315 alumnos del 3º al 5º año de la enseñanza básica, de escuelas públicas, cuyas edades variaron de 8 a 13 años, siendo 169 niños. Los puntajes obtenidos con la medida de evaluación de la consciencia metatextual (QACM fueron convergentes con los de la comprensión de lectura y el instrumento se mostró sensible para captar el desarrollo de los niños en identificar los géneros textuales con el avance de la escolaridad. De esa forma, el estudio forneció evidencias de validez por la convergencia de constructos, identificada con base en las medidas relacionadas, como de criterio, distinguiendo los alumnos por su nivel de escolaridad.The stated goal of this

  12. Radon-enriched spring waters in the South of Poland

    International Nuclear Information System (INIS)

    Kozlowska, B.; Hetman, A.; Dorda, J.; Zipper, W.

    2001-01-01

    A method for determination of 222 Rn in natural water samples which involves a Wallac 1414 Win Spectral α/β liquid scintillation counter is described. Samples were collected from springs in health resorts in the Sudety Mountains in Poland. Half of the studied water samples were radon enriched with an activity concentration higher then 74 Bq/l. Seasonal variations of 222 Rn in these waters are under investigation. The method introduced is very convenient and elegant for radon activity measurements.

  13. Thermogenic Effect from Nutritionally Enriched Coffee Consumption

    Directory of Open Access Journals (Sweden)

    Jennings Peter F

    2006-06-01

    Full Text Available Abstract Background The purpose of this study was to examine the effect of nutritionally enriched JavaFit™ (JF coffee (450 mg of caffeine, 1200 mg of garcinia cambogia, 360 mg of citrus aurantium extract, and 225 mcg of chromium polynicotinate on resting oxygen uptake (VO2, respiratory exchange ratio (RER, heart rate (HR, and blood pressure (BP in healthy and physically active individuals. Method Ten subjects (8 male, 2 female; 20.9 ± 1.7 y; 178.1 ± 10.4 cm; 71.8 ± 12.1 kg underwent two testing sessions administered in a randomized and double-blind fashion. During each session, subjects reported to the Human Performance Laboratory after at least 3-h post-absorptive state and were provided either 354 ml (1.5 cups of freshly brewed JF or commercially available caffeinated coffee (P. Subjects then rested in a semi-recumbent position for three hours. VO2 and HR were determined every 5 min during the first 30 min and every 10 min during the next 150 min. BP was determined every 15 min during the first 30 min and every 30 min thereafter. Area under the curve (AUC analysis was computed for VO2, whereas a session-average was calculated for RER, HR and BP. Results Initial analysis revealed no significant differences. However, seven of the ten subjects were considered responders to JF (had a higher AUC for VO2during JF than P. Statistical analysis showed the difference between JF and P (12% to be significantly different in these responders. In addition, the average systolic BP was higher (p Conclusion It appears that consuming a nutritionally-enriched coffee beverage may increase resting energy expenditure in individuals that are sensitive to the caffeine and herbal coffee supplement. In addition, this supplement also appears to affect cardiovascular dynamics by augmenting systolic arterial blood pressure.

  14. Nondestructive determination of uranium-235 enrichment in gas ultracentrifugation enrichment plants

    International Nuclear Information System (INIS)

    Lauppe, W.D.; Richter, B.; Stein, G.

    1990-02-01

    Based on similar studies in the USA and the UK, two γ spectroscopic techniques were propagated by the IAEA, after they had been sucesssfully tried at Capenhurst (GB) centrifuge plant. It was assumed by the IAEA that these methods would be transferable to the Almelo and Gronau plants, even without knowledge of the specific plant parameters there. The scope of this research project covered the study and further development of the proposed γ-spectroscopic measuring methods for applicability in the GUC plant in Gronau. The research came within the terms of reference of Task C.14.7 of the IAEA and BMFT (German Federal Ministry of Research and Technology)'s joint research and development programme. When the funded project first started, the Gronau plant was not yet on stream. The initial measurements were therefore, taken in Almelo, where conditions were similar to those in Gronau. The report describes the underlying problems which occur in relation to the non-destructive measuring of 235 U enrichment under the specific boundary conditions in Almelo and Gronau. The results illustrate the limitations of application of the measuring techniques, even after adjustment to actual conditions in Almelo and Gronau. It is not always possible to obtain a reliable yes/no answer in favour of slightly enriched uranium, particularly in product lines with extreme boundary conditions, irrespective of the technique applied. (orig.) [de

  15. Assessing locomotion deficiency in broiler chicken Medida de deficiência locomotora em frango de corte

    Directory of Open Access Journals (Sweden)

    Irenilza de Alencar Nääs

    2010-04-01

    parte horizontal da área de passagem foi colocado um tapete fino, com elementos sensores de cristal piezoelétrico, para registrar o pico de força vertical das patas do frango quando este caminhava sobre a plataforma. O sistema de mensuração consistiu de um tapete com sensores eletrônicos e um programa computacional que permitiu o registro de forças em tempo real e o processamento e a análise de dados. Imagens de vídeo foram tomadas a partir de duas câmeras digitais para atribuir o gait score. Quarenta frangos machos foram aleatoriamente selecionados em condições similares de alojamento e da mesma granja, com idade variando de 49 a 28 dias (dez de cada idade e usados no experimento. A medida consistiu em induzir a ave a caminhar sobre a plataforma de força que automaticamente registrava o pico de força da passada. O gait score aumentou com o peso e a idade das aves. Foi encontrada assimetria nos picos de força da cada pata, independente da idade ou gait score. Embora não tenha sido identificado visualmente nos frangos, o pico de força vertical é diferente tanto na pata direita quanto na esquerda, levando a um caminhar lento e desigual. A deficiência locomotora foi mais intensa em aves mais velhas.

  16. Medidas procesales especiales y proteccíon de los derechos humanos
    Informe general

    Directory of Open Access Journals (Sweden)

    John A.E. Vervaele

    2009-10-01

    Full Text Available El objetivo del informe general es realizar un análisis comparativo de los informes nacionales para evidenciar los procesos de transformación de los sistemas nacionales de justicia penal, en particular del proceso penal, en el que se han introducido medidas procesales especiales para hacer frente al terrorismo y al crimen organizado, y determinar si esto ha llevado a los países a apartarse de sus propias normas fundamentales, procedimientos, principios y estándares de derechos humanos aplicables. Partiendo de la premisa de que el sistema integrado de derecho penal tiene tres dimensiones – la protección de las personas (la dimensión de escudo, la disposición de instrumentos de aplicación de la ley (la dimensión de la espada y de pesos y contrapesos / trias politica (la dimensión constitucional – el informe ofrece un panorama general de las transformaciones relacionadas entre sí, principalmente en la fase previa al juicio, que han afectado a tales dimensiones como consecuencia de tres oleadas de ‘guerras’ (contra las drogas, el crimen organizado y el terrorismo. En muchos países, las garantías procesales y los principios que protegen frente a violaciones del derecho a un juicio justo son considerados una carga para la eficacia de la persecución de los delitos graves. Estas reformas han dado lugar a una clara expansión del estado punitivo y a que queden difuminadas las distinciones clásicas, y no favorecen al estado de derecho. El actuar centrado en la seguridad pública y en la investigación coactiva preventiva socava el sistema de justicia penal. Con un sistema de justicia penal utilizado crecientemente como instrumento para regular el presente y/o el futuro en lugar de para castigar el comportamiento pasado, y un proceso penal en el que la investigación previa al juicio no se dirige a conocer la verdad en relación con el delito cometido, sino a la construcción y de-construcción de la peligrosidad social, los

  17. Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing.

    Science.gov (United States)

    Thoendel, Matthew; Jeraldo, Patricio R; Greenwood-Quaintance, Kerryl E; Yao, Janet Z; Chia, Nicholas; Hanssen, Arlen D; Abdel, Matthew P; Patel, Robin

    2016-08-01

    Metagenomic whole genome sequencing for detection of pathogens in clinical samples is an exciting new area for discovery and clinical testing. A major barrier to this approach is the overwhelming ratio of human to pathogen DNA in samples with low pathogen abundance, which is typical of most clinical specimens. Microbial DNA enrichment methods offer the potential to relieve this limitation by improving this ratio. Two commercially available enrichment kits, the NEBNext Microbiome DNA Enrichment Kit and the Molzym MolYsis Basic kit, were tested for their ability to enrich for microbial DNA from resected arthroplasty component sonicate fluids from prosthetic joint infections or uninfected sonicate fluids spiked with Staphylococcus aureus. Using spiked uninfected sonicate fluid there was a 6-fold enrichment of bacterial DNA with the NEBNext kit and 76-fold enrichment with the MolYsis kit. Metagenomic whole genome sequencing of sonicate fluid revealed 13- to 85-fold enrichment of bacterial DNA using the NEBNext enrichment kit. The MolYsis approach achieved 481- to 9580-fold enrichment, resulting in 7 to 59% of sequencing reads being from the pathogens known to be present in the samples. These results demonstrate the usefulness of these tools when testing clinical samples with low microbial burden using next generation sequencing. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. 76 FR 387 - Atomic Safety and Licensing Board; AREVA Enrichment Services, LLC (Eagle Rock Enrichment Facility)

    Science.gov (United States)

    2011-01-04

    ... and Licensing Board; AREVA Enrichment Services, LLC (Eagle Rock Enrichment Facility) December 17, 2010... construction and operation of a gas centrifuge uranium enrichment facility--denoted as the Eagle Rock... site at http://www.nrc.gov/materials/fuel-cycle-fac/arevanc.html . These and other documents relating...

  19. Medidas de actuación para la prevención de la toxiinfección alimentaria

    Directory of Open Access Journals (Sweden)

    María Ascensión Maestre Naranjo

    2008-09-01

    Full Text Available La toxiinfección alimentaria manifestada en forma de brotes tiene gran importancia desde el punto de vista epidemiológico. Tras analizar su etiología se instauran las medidas preventivas y de seguridad en todos los procesos de manipulación de alimentos, así como las medidas de prevención para los trabajadores manipuladores de alimentos. Se insiste de forma especial en la información y formación que deben recibir estos trabajadores, para concienciar y fomentar su responsabilidad y educación en las medidas higiénico sanitarias que favorecen su desarrollo. En caso de producirse el brote se marcan las pautas a seguir para su notificación, por la gran importancia legal y epidemiológica que comporta.The alimentary toxiinfección manifested in form of buds has great importance from the epidemic point of view. After analyzing their etiología the preventive measures they are established and of security in all the processes of manipulation of foods, as well as the measures of prevention for the hard-working manipulators of foods. It is insisted in a special way in the information and formation that these workers should receive, to make aware and to foment their responsibility and education in the hygienic sanitary measures that favor their development. In the event of taking place the bud the rules they are marked to continue for their notification, for the legal and epidemic great importance that behaves.

  20. Medidas de conservación y ordenación pesquera del jurel Trachurus murphyien el Perú

    Directory of Open Access Journals (Sweden)

    Jorge Zuzunaga

    2013-10-01

    Full Text Available Las pesquerías peruanas como actividades realizadas sobre recursos naturales renovables son reguladas por un marco jurídico, cuyo objetivo es asegurar su conservación y uso sostenible. La Ley General de Pesca (DL N°. 25977 señala que para cada tipo de pesquería y según la situación del recurso explotado debe establecerse un sistema de ordenamiento que concilie el principio de sostenibilidad de los recursos pesqueros o conservación en el largo plazo, con la obtención de los mayores beneficios económicos y sociales. En el ordenamiento pesquero del jurel Trachurus murphyi, las medidas incluyen entre otras, el régimen de acceso constituido por las autorizaciones de incremento de flota y permisos de pesca, que se otorgan solo a los buques cuyas capturas son destinadas únicamente para el consumo humano directo, de acuerdo a lo dispuesto en el Reglamento de Ordenamiento Pesquero (DS N.° 011-2007-PRODUCE. La obligación de destinar los recursos sardina Sardinops sagax, jurel T. murphyiy caballa Scomber japonicus al consumo humano directo rige desde septiembre 2002 (DS N°. 001-2002-PRODUCE. Otras medidas vigentes restringen el tamaño mínimo de malla de las redes, prohíben la captura de ejemplares menores de 31 cm de longitud total, determinan el inicio y cierre de las temporadas de pesca, los límites de captura o cuotas anuales. Estas disposiciones son complementadas por medidas de seguimiento, control y vigilancia para la efectiva conservación y uso sostenido del recurso.