WorldWideScience

Sample records for efficient nuclear targeting

  1. Efficient Subcellular Targeting to the Cell Nucleus of Quantum Dots Densely Decorated with a Nuclear Localization Sequence Peptide.

    Science.gov (United States)

    Maity, Amit Ranjan; Stepensky, David

    2016-01-27

    Organelle-targeted drug delivery can enhance the efficiency of the intracellularly acting drugs and reduce their toxicity. We generated core-shell type CdSe-ZnS quantum dots (QDs) densely decorated with NLS peptidic targeting residues using a 3-stage decoration approach and investigated their endocytosis and nuclear targeting efficiencies. The diameter of the generated QDs increased following the individual decoration stages (16.3, 18.9, and 21.9 nm), the ζ-potential became less negative (-33.2, -17.5, and -11.9 mV), and characteristic changes appeared in the FTIR spectra following decoration with the linker and NLS peptides. Quantitative analysis of the last decoration stage revealed that 37.9% and 33.2% of the alkyne-modified NLS groups that were added to the reaction mix became covalently attached or adsorbed to the QDs surface, respectively. These numbers correspond to 63.6 and 55.7 peptides conjugated or adsorbed to a single QD (the surface density of 42 and 37 conjugated and adsorbed peptides per 1000 nm(2) of the QDs surface), which is higher than in the majority of previous studies that reported decoration efficiencies of formulations intended for nuclear-targeted drug delivery. QDs decorated with NLS peptides undergo more efficient endocytosis, as compared to other investigated QDs formulations, and accumulated to a higher extent in the cell nucleus or in close vicinity to it (11.9%, 14.6%, and 56.1% of the QDs endocytosed by an average cell for the QD-COOH, QD-azide, and QD-NLS formulations, respectively). We conclude that dense decoration of QDs with NLS residues increased their endocytosis and led to their nuclear targeting (preferential accumulation in the cells nuclei or in close vicinity to them). The experimental system and research tools that were used in this study allow quantitative investigation of the mechanisms that govern the QDs nuclear targeting and their dependence on the formulation properties. These findings will contribute to the

  2. China's energy efficiency target 2010

    International Nuclear Information System (INIS)

    Yang Ming

    2008-01-01

    The Chinese government has set an ambitious target: reducing China's energy intensity by 20%, or 4.36% each year between 2006 and 2010 on the 2005 level. Real data showed that China missed its target in 2006, having reduced its energy intensity only by 1.3%. The objective of this study is to evaluate the feasibility and potential of the Chinese to achieve the target. This paper presents issues of macro-economy, population migration, energy savings, and energy efficiency policy measures to achieve the target. A top-down approach was used to analyse the relationship between the Chinese economic development and energy demand cycles and to identify the potentials of energy savings in sub-sectors of the Chinese economy. A number of factors that contribute to China's energy intensity are identified in a number of energy-intensive sectors. This paper concludes that China needs to develop its economy at its potential GDP growth rate; strengthen energy efficiency auditing, monitoring and verification; change its national economy from a heavy-industry-dominated mode to a light industry or a commerce-dominated mode; phase out inefficient equipment in industrial sectors; develop mass and fast railway transportation; and promote energy-efficient technologies at the end use. This paper transfers key messages to policy makers for designing their policy to achieve China's energy efficiency target

  3. Preparation of thin nuclear targets

    International Nuclear Information System (INIS)

    Muggleton, A.H.F.

    1979-03-01

    Thin film backings, sources and targets are needed for many applications in low energy nuclear physics and nuclear chemistry experiments. A survey of techniques used in the preparation of nuclear targets is first briefly discussed. These are classified as chemical, mechanical and physical preparations. Vacuum evaporation, being the most generally used technique, is discussed in detail. It is highly desirable to monitor the film thickness and control the deposition rate during evaporation and to measure the final target thickness after deposition has concluded. The relative merits of various thickness measuring techniques are described. Stages in the fabrication and mounting of self-supporting foils are described in detail, with emphasis given to the preparation of thin self-supporting carbon foils used as target backings and stripper foils. Various target backings, and the merits of the more generally used release agents are described in detail. The preparations of more difficult elemental targets are discussed, and a comprehensive list of the common targets is presented

  4. Particle physics using nuclear targets

    International Nuclear Information System (INIS)

    Ferbel, T.

    1978-01-01

    The use of nuclear targets in particle physics is discussed and some recent results obtained in studies of hadronic interactions on nuclei summarized. In particular experimental findings on inclusive production and on coherent dissociation of mesons and baryons at high energies are presented. 41 references

  5. Molecular nuclear imaging for targeting and trafficking

    International Nuclear Information System (INIS)

    Bom, Hee Seung; Min, Jung Jun; Jeong, Hwan-Jeong

    2006-01-01

    Noninvasive molecular targeting in living subjects is highly demanded for better understanding of such diverse topics as the efficient delivery of drugs, genes, or radionuclides for the diagnosis or treatment of diseases. Progress in molecular biology, genetic engineering and polymer chemistry provides various tools to target molecules and cells in vivo. We used chitosan as a polymer, and 99m Tc as a radionuclide. We developed 99m Tc-galactosylated chitosan to target asialoglycoprotein receptors for nuclear imaging. We also developed 99m Tc-HYNIC-chitosan-transferrin to target inflammatory cells, which was more effective than 67 Ga-citrate for imaging inflammatory lesions. For an effective delivery of molecules, a longer circulation time is needed. We found that around 10% PEGylation was most effective to prolong the circulation time of liposomes for nuclear imaging of 99m Tc-HMPAO-labeled liposomes in rats. Using various characteristics of molecules, we can deliver drugs into targets more effectively. We found that 99m Tc-labeled biodegradable pullulan-derivatives are retained in tumor tissue in response to extracellular ion-strength. For the trafficking of various cells or bacteria in an intact animal, we used optical imaging techniques or radiolabeled cells. We monitored tumor-targeting bacteria by bioluminescent imaging techniques, dentritic cells by radiolabeling and neuronal stem cells by sodium-iodide symporter reporter gene imaging. In summary, we introduced recent achievements of molecular nuclear imaging technologies in targeting receptors for hepatocyte or inflammatory cells and in trafficking bacterial, immune and stem cells using molecular nuclear imaging techniques

  6. Nuclear liability, nuclear safety, and economic efficiency

    International Nuclear Information System (INIS)

    Wood, W.C.

    1980-01-01

    This dissertation applies the methods of economic analysis to nuclear liability and Price-Anderson. First the legislative history is reviewed; in that history the economic role of liability in affecting safety and allocating risk was virtually ignored. Succeeding chapters reformulate issues from the policy debate and subject them to economic analysis. A persistent issue is whether nuclear utilities respond to their limited liability by allowing a higher probability of serious accident. Comparative-static analysis shows that limited liability does lead to a higher chance of accidents, though the effect may be small. The analysis also shows that safety is achieved in a more capital-intensive manner than is cost-minimizing and that limited liability causes reactor owners to favor more heavily populated sites for plants. Therefore, the siting decision makes potential loss greater even if there is no change in the probability of an accident. Citizens' preferences on nuclear liability are examined next, starting with the nature of coverage that would be just in the sense of contraction theories such as John Rawls' Theory of Justice. Citizens behind Rawls' veil of ignorance, forced to be fair because of their ignorance of whether they will be harmed, unanimously choose a high level of coverage. The just level of coverage is greater than the existing $560 million. Second, the nature of economically efficient liability coverage is determined and contrasted with coverage that would emerge from a democratic system of public choice. Population and expected damage profiles indicate that majorities could easily be formed among groups of citizens expecting to suffer little of the damage of a nuclear accident. Thus, majority voting on liability arrangements is likely to produce an inefficiently low level of coverage

  7. Nuclear spin polarization of targets

    International Nuclear Information System (INIS)

    Happer, W.

    1990-01-01

    Lasers can be used to produce milligrams to grams of noble gas nuclei with spin polarizations in excess of 50%. These quantities are sufficient to be very useful targets in nuclear physics experiments. Alkali-metal atoms are used to capture the angular momentum of circularly polarized laser photons, and the alkali-metal atoms transfer their angular momentum to noble gas atoms in binary or three-body collisions. Non-radiative collisions between the excited alkali atoms and molecular quenching gases are essential to avoid radiation trapping. The spin exchange can involve gas-phase van der Waals molecules, consisting of a noble gas atom and an alkali metal atom. Surface chemistry is also of great importance in determining the wall-induced relaxation rates of the noble gases

  8. Polarized targets at triangle universities nuclear laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Seely, M.L. [North Carolina State Univ., Raleigh, NC (United States); Gould, C.R. [North Carolina State Univ., Raleigh, NC (United States); Haase, D.G. [North Carolina State Univ., Raleigh, NC (United States); Huffman, P.R. [Triangle Universities Nuclear Laboratory, Durham, NC 27708 (United States); Keith, C.D. [North Carolina State Univ., Raleigh, NC (United States); Roberson, N.R. [Triangle Universities Nuclear Laboratory, Durham, NC 27708 (United States); Tornow, W. [Triangle Universities Nuclear Laboratory, Durham, NC 27708 (United States); Wilburn, W.S. [Triangle Universities Nuclear Laboratory, Durham, NC 27708 (United States)

    1995-03-01

    A summary of the polarized and aligned nuclear targets which have been constructed and used at the Triangle Universities Nuclear Laboratory is given. Statically polarized targets, typically operating at a temperature of 12 mK and a magnetic field of 7 T, have provided significant nuclear polarization in {sup 1}H, {sup 3}He, {sup 27}Al, {sup 93}Nb and {sup 165}Ho. A rotating, aligned {sup 165}Ho target is also in use. A {sup 3}He melting curve thermometer has been developed for use in statically polarized targets. A dynamically polarized proton target is under construction. ((orig.))

  9. Safety targets for nuclear power plants

    International Nuclear Information System (INIS)

    Herttrich, P.M.

    1985-01-01

    By taking as an example the safety targets of the American nuclear energy authority US-NRC, this paper explains what is meant by global, quantitative safety targets for nuclear power plants and what expectations are associated with the selecton of such safety targets. It is shown how probabilistic methods can be an appropriate completion of proven deterministic methods and what are the sectors where their application may become important in future. (orig./HP) [de

  10. High-efficiency target-ion sources for RIB generation

    International Nuclear Information System (INIS)

    Alton, G.D.

    1993-01-01

    A brief review is given of high-efficiency ion sources which have been developed or are under development at ISOL facilities which show particular promise for use at existing, future, or radioactive ion beam (RIB) facilities now under construction. Emphasis will be placed on those sources which have demonstrated high ionization efficiency, species versatility, and operational reliability and which have been carefully designed for safe handling in the high level radioactivity radiation fields incumbent at such facilities. Brief discussions will also be made of the fundamental processes which affect the realizable beam intensities in target-ion sources. Among the sources which will be reviewed will be selected examples of state-of-the-art electron-beam plasma-type ion sources, thermal-ionization, surface-ionization, ECR, and selectively chosen ion source concepts which show promise for radioactive ion beam generation. A few advanced, chemically selective target-ion sources will be described, such as sources based on the use of laser-resonance ionization, which, in principle, offer a more satisfactory solution to isobaric contamination problems than conventional electromagnetic techniques. Particular attention will be given to the sources which have been selected for initial or future use at the Holifield Radioactive Ion Beam Facility now under construction at the Oak Ridge National Laboratory

  11. Static nuclear polarisation and polarised targets

    International Nuclear Information System (INIS)

    Heeringa, W.

    1984-12-01

    Recent progress and status of statically polarised nuclear targets are reviewed. Special attention is given to polarised 1 H and 3 He. An important quantity in the determination of the target polarisation is the thermal gradient over the target sample. The dependence of this gradient on heat input, sample geometry, and thermal conductivity of the sample is discussed. Possibilities of performing experiments with proton beams are indicated. (orig.) [de

  12. Nuclear Targeting Terms for Engineers and Scientists

    Energy Technology Data Exchange (ETDEWEB)

    St Ledger, John W. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-02-01

    The Department of Defense has a methodology for targeting nuclear weapons, and a jargon that is used to communicate between the analysts, planners, aircrews, and missile crews. The typical engineer or scientist in the Department of Energy may not have been exposed to the nuclear weapons targeting terms and methods. This report provides an introduction to the terms and methodologies used for nuclear targeting. Its purpose is to prepare engineers and scientists to participate in wargames, exercises, and discussions with the Department of Defense. Terms such as Circular Error Probable, probability of hit and damage, damage expectancy, and the physical vulnerability system are discussed. Methods for compounding damage from multiple weapons applied to one target are presented.

  13. Ownership and efficiency in nuclear power production

    International Nuclear Information System (INIS)

    Pollitt, M.G.

    1995-01-01

    This paper aims to contribute to the relatively small amount of academic literature on the efficiency of nuclear power production. The author draws on world-wide comparisons to illustrate the situation in the United Kingdom, where the nuclear generating capacity, conceived of and constructed as a public concern, has recently been privatised. The theory and evidence for links between ownership and productive efficiency is received. Efficiency measures used are explained as are the linear programs required to generate them. Data Envelopment Analysis (DEA) is used to analyse productive efficiency of nuclear power plants before and after privatisation. Results of the DEA are used to test the hypothesis that ownership has no effect on productive efficiency. (UK)

  14. Target-ion source unit ionization efficiency measurement by method of stable ion beam implantation

    CERN Document Server

    Panteleev, V.N; Fedorov, D.V; Moroz, F.V; Orlov, S.Yu; Volkov, Yu.M

    The ionization efficiency is one of the most important parameters of an on-line used target-ion source system exploited for production of exotic radioactive beams. The ionization efficiency value determination as a characteristic of a target-ion source unit in the stage of its normalizing before on-line use is a very important step in the course of the preparation for an on-line experiment. At the IRIS facility (Petersburg Nuclear Physics Institute, Gatchina) a reliable and rather precise method of the target-ion source unit ionization efficiency measurement by the method of stable beam implantation has been developed. The method worked out exploits an off-line mass-separator for the implantation of the ion beams of selected stable isotopes of different elements into a tantalum foil placed inside the Faraday cup in the focal plane of the mass-separator. The amount of implanted ions has been measured with a high accuracy by the current integrator connected to the Faraday cup. After the implantation of needed a...

  15. High efficiency diffusion molecular retention tumor targeting.

    Directory of Open Access Journals (Sweden)

    Yanyan Guo

    Full Text Available Here we introduce diffusion molecular retention (DMR tumor targeting, a technique that employs PEG-fluorochrome shielded probes that, after a peritumoral (PT injection, undergo slow vascular uptake and extensive interstitial diffusion, with tumor retention only through integrin molecular recognition. To demonstrate DMR, RGD (integrin binding and RAD (control probes were synthesized bearing DOTA (for (111 In(3+, a NIR fluorochrome, and 5 kDa PEG that endows probes with a protein-like volume of 25 kDa and decreases non-specific interactions. With a GFP-BT-20 breast carcinoma model, tumor targeting by the DMR or i.v. methods was assessed by surface fluorescence, biodistribution of [(111In] RGD and [(111In] RAD probes, and whole animal SPECT. After a PT injection, both probes rapidly diffused through the normal and tumor interstitium, with retention of the RGD probe due to integrin interactions. With PT injection and the [(111In] RGD probe, SPECT indicated a highly tumor specific uptake at 24 h post injection, with 352%ID/g tumor obtained by DMR (vs 4.14%ID/g by i.v.. The high efficiency molecular targeting of DMR employed low probe doses (e.g. 25 ng as RGD peptide, which minimizes toxicity risks and facilitates clinical translation. DMR applications include the delivery of fluorochromes for intraoperative tumor margin delineation, the delivery of radioisotopes (e.g. toxic, short range alpha emitters for radiotherapy, or the delivery of photosensitizers to tumors accessible to light.

  16. Dynamic nuclear polarization of irradiated target materials

    International Nuclear Information System (INIS)

    Seely, M.L.

    1982-01-01

    Polarized nucleon targets used in high energy physics experiments usually employ the method of dynamic nuclear polarization (DNP) to polarize the protons or deuterons in an alcohol. DNP requires the presence of paramagnetic centers, which are customarily provided by a chemical dopant. These chemically doped targets have a relatively low polarizable nucleon content and suffer from loss of polarization when subjected to high doses of ionizing radiation. If the paramagnetic centers formed when the target is irradiated can be used in the DNP process, it becomes possible to produce targets using materials which have a relatively high polarizable nucleon content, but which are not easily doped by chemical means. Furthermore, the polarization of such targets may be much more radiation resistant. Dynamic nuclear polarization in ammonia, deuterated ammonia, ammonium hydroxide, methylamine, borane ammonia, butonal, ethane and lithium borohydride has been studied. These studies were conducted at the Stanford Linear Accelerator Center using the Yale-SLAC polarized target system. Results indicate that the use of ammonia and deuterated ammonia as polarized target materials would make significant increases in polarized target performance possible

  17. Mass Producing Targets for Nuclear Fusion

    Science.gov (United States)

    Wang, T. G.; Elleman, D. D.; Kendall, J. M.

    1983-01-01

    Metal-encapsulating technique advances prospects of controlling nuclear fusion. Prefilled fusion targets form at nozzle as molten metal such as tin flows through outer channel and pressurized deuterium/tritium gas flows through inner channel. Molten metal completely encloses gas charge as it drops off nozzle.

  18. 210Pb targets for nuclear spectroscopy

    International Nuclear Information System (INIS)

    Maier, H.J.; Grossmann, R.

    1987-01-01

    The preparation of metallic 210 Pb targets by vacuum evaporation condensation is described. Lead-210, which is highly radioactive, is available as nitrate in 3M HNO 3 . Solid Pb(NO 3 ) 2 is extracted from this solution and subjected to a reductive evaporation procedure. A special-shaped evaporation crucible yields a collection efficiency of 2.8 mg/cm 2 per milligram of employed material. (orig.)

  19. Development and Targeting Efficiency of Irinotecan Engineered ...

    African Journals Online (AJOL)

    Erah

    Conclusion: Proniosomes offer a suitable alternative colloidal carrier approach to achieving drug ... for the treatment of localized disease in the body ... analogue of the natural alkaloid, campto- ..... vasculature targeted tumor necrosis factor-α.

  20. Target Improves Efficiency in New Construction

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-03-01

    Target Corporation partnered with the U.S. Department of Energy (DOE) to develop and implement solutions to reduce annual energy consumption in new stores by at least 50% versus requirements set by ASHRAE/ANSI/IESNA Standard 90.1-20041 as part of DOE’s Commercial Building Partnership (CBP) program.

  1. Model France. Efficiently achieving climate protection targets. Nuclear power phase-out scenario adapted in favour of climate protection; Vorbild Frankreich. Klimaschutzziele effizient erreichen. Ausstiegszenario Kernkraft zugunsten des Klimaschutzes angepasst

    Energy Technology Data Exchange (ETDEWEB)

    Leidinger, Tobias [Luther Rechtsanwaltsgesellschaft, Duesseldorf (Germany)

    2017-12-15

    In Germany - after an abrupt, legally controversial and in some cases unconstitutional phase-out of nuclear power (''at any price'') - the next phase-out of is now being negotiated among new coalition partners. In France, on the other hand, energy and climate protection seem to be in the clear sense of the word: the French nuclear phase-out scenario is clearly being ''stretched out'' in order to achieve the promised climate protection targets. Reason seems to have the upper hand in France: instead of ''black-and-white scenarios'', one prefers to stick to what is in reality feasible and achievable. In reality, the expansion of renewable energies, the regulation of the transport sector and electricity production from nuclear energy are not opposites, but complement each other in the interests of affordable energy and effective climate protection.

  2. Nuclear targets, recoil ion catchers and reaction chambers

    NARCIS (Netherlands)

    Dionisio, JS; Vieu, C; Schuck, C; Collatz, R; Meunier, R; Ledu, D; Folger, H; Lafoux, A; Lagrange, JM; Pautrat, M; Waast, B; Phillips, WR; Blunt, D; Durell, JL; Varley, BJ; Dagnall, PG; Dorning, SJ; JONES, MA; Smith, AG; Bacelar, JCS; Rzaca-Urban, T; Amzal, N; Meliani, Z; Vanhorenbeeck, J; Passoja, A; Urban, W

    1998-01-01

    The main features of nuclear targets, recoil ion catchers and reaction chambers used in nuclear spectroscopic investigations involving in-beam multi-e-gamma spectrometers are discussed. The relative importance of the F-ray background due to the accelerated ion-target and the recoil-ion-target

  3. Nuclear Security: Target Analysis-rev

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Surinder Paul [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Gibbs, Philip W. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bultz, Garl A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2014-03-01

    The objectives of this presentation are to understand target identification, including roll-up and protracted theft; evaluate target identification in the SNRI; recognize the target characteristics and consequence levels; and understand graded safeguards.

  4. Nuclear physics with internal targets in electron storage rings

    International Nuclear Information System (INIS)

    Holt, R.J.

    1986-01-01

    Two key experiments in nuclear physics will be discussed in order to illustrate the advantages of the internal target method and demonstrate the power of polarization techniques in electron scattering studies. The progress of internal target experiments will be discussed and the technology of internal polarized target development will be reviewed. 43 refs., 11 figs

  5. Nuclear performance standards: Promoting efficient generation

    International Nuclear Information System (INIS)

    Nagelhout, M.

    1990-01-01

    Nuclear plant performance standards are designed to share the risks of operation associated with nuclear generation. Such standards often shift risks from ratepayers to utility shareholders, even without a finding of imprudence or mismanagement. The rationale underlying nuclear performance standards is that ratepayers should not be responsible for excessive replacement power costs incurred as a result of unreasonable decisions by utility management, especially because the high fixed costs of nuclear plants are already included in base rates. In addition, performance standards can be designed to provide incentives to reward utilities that achieve superior nuclear performance, for the benefit of both ratepayers and shareholders

  6. Computational design of high efficiency release targets for use at ISOL facilities

    CERN Document Server

    Liu, Y

    1999-01-01

    This report describes efforts made at the Oak Ridge National Laboratory to design high-efficiency-release targets that simultaneously incorporate the short diffusion lengths, high permeabilities, controllable temperatures, and heat-removal properties required for the generation of useful radioactive ion beam (RIB) intensities for nuclear physics and astrophysics research using the isotope separation on-line (ISOL) technique. Short diffusion lengths are achieved either by using thin fibrous target materials or by coating thin layers of selected target material onto low-density carbon fibers such as reticulated-vitreous-carbon fiber (RVCF) or carbon-bonded-carbon fiber (CBCF) to form highly permeable composite target matrices. Computational studies that simulate the generation and removal of primary beam deposited heat from target materials have been conducted to optimize the design of target/heat-sink systems for generating RIBs. The results derived from diffusion release-rate simulation studies for selected t...

  7. Differentiated influences of risk perceptions on nuclear power acceptance according to acceptance targets: Evidence from Korea

    Directory of Open Access Journals (Sweden)

    Seungkook Roh

    2017-08-01

    Full Text Available The determinants of the public's nuclear power acceptance have received considerable attention as decisive factors regarding nuclear power policy. However, the contingency of the relative importance of different determinants has been less explored. Building on the literature of psychological distance between the individual and the object, the present study demonstrates that the relative effects of different types of perceived risks regarding nuclear power generation differ across acceptance targets. Using a sample of Korea, our results show that, regarding national acceptance of nuclear power generation, perceived risk from nuclear power plants exerts a stronger negative effect than that from radioactive waste management; however, the latter exerts a stronger negative effect than the former on local acceptance of a nuclear power plant. This finding provides implications for efficient public communication strategy to raise nuclear power acceptance.

  8. Differentiated influences of risk perceptions on nuclear power acceptance according to acceptance targets: Evidence from Korea

    International Nuclear Information System (INIS)

    Roh, Seung Kook; Lee, Jin Won

    2017-01-01

    The determinants of the public's nuclear power acceptance have received considerable attention as decisive factors regarding nuclear power policy. However, the contingency of the relative importance of different determinants has been less explored. Building on the literature of psychological distance between the individual and the object, the present study demonstrates that the relative effects of different types of perceived risks regarding nuclear power generation differ across acceptance targets. Using a sample of Korea, our results show that, regarding national acceptance of nuclear power generation, perceived risk from nuclear power plants exerts a stronger negative effect than that from radioactive waste management; however, the latter exerts a stronger negative effect than the former on local acceptance of a nuclear power plant. This finding provides implications for efficient public communication strategy to raise nuclear power acceptance

  9. Differentiated influences of risk perceptions on nuclear power acceptance according to acceptance targets: Evidence from Korea

    Energy Technology Data Exchange (ETDEWEB)

    Roh, Seung Kook [Policy Research Center, Korea Atomic Energy Research Institute (KAERI), Daejeon (Korea, Republic of); Lee, Jin Won [School of Management, Xiamen University, Xiamen (China)

    2017-08-15

    The determinants of the public's nuclear power acceptance have received considerable attention as decisive factors regarding nuclear power policy. However, the contingency of the relative importance of different determinants has been less explored. Building on the literature of psychological distance between the individual and the object, the present study demonstrates that the relative effects of different types of perceived risks regarding nuclear power generation differ across acceptance targets. Using a sample of Korea, our results show that, regarding national acceptance of nuclear power generation, perceived risk from nuclear power plants exerts a stronger negative effect than that from radioactive waste management; however, the latter exerts a stronger negative effect than the former on local acceptance of a nuclear power plant. This finding provides implications for efficient public communication strategy to raise nuclear power acceptance.

  10. Efficiency of an LBE spallation target in an accelerator-driven molten salt subcritical reactor

    Energy Technology Data Exchange (ETDEWEB)

    Bak, Sang-In [Sungkyunkwan University, Suwon (Korea, Republic of); Hong, Seung-Woo [Sungkyunkwan University, Suwon (Korea, Republic of); Kadi, Yacine [CERN, Geneva (Switzerland)

    2016-10-15

    An Accelerator-Driven System (ADS) combined with a subcritical Molten Salt Reactor (MSR) is a type of hybrid reactor originally designed to breed uranium from thorium or to incinerate long-lived minor actinides in nuclear wastes. In an MSR, the salt material is used not only as a nuclear fuel but also as a primary coolant. In addition, this material is used as a target for inducing spallation neutrons in most AD-MSR concepts. A high energy proton beam impinges on a heavy metal target to induce spallation reactions and produces neutrons. Accordingly, a reliable proton accelerator is needed to feed the source neutrons. As ADSs have been criticized for requiring high power accelerators, minimization of beam power is an important aspect of ADS design. A primary concern associated with ADS development is stable high-power accelerators. We therefore studied the neutron source efficiencies of an AD-MSR involving chloride fuels by including a Pb-Bi eutectic (LBE) spallation target. The proton source efficiency and the accelerator beam power required have been studied for an AD-MSR. Adoption of an LBE spallation target induces an increase in proton source efficiencies in comparison to the case without a spallation target. Thus the presence of an efficient spallation target is useful in the reduction of the beam power of an accelerator. Almost 33 % of the beam power can be reduced in comparison to the case without the target for NaCl-Th/{sup 233}U fuel, and about 16 % for NaCl-U/TRU fuel. The beam power amplifications increase by 1.5 times for NaCl-Th/{sup 233}U and 1.2 times for NaCl-U/TRU in comparison with the no target AD-MSR.

  11. Long-term cost targets for nuclear energy

    International Nuclear Information System (INIS)

    Rogner, H.H.; McDonald, A.

    2004-01-01

    In 2000 the International Atomic Energy Agency (IAEA) began the International Project on Innovative Nuclear Reactors and Fuel Cycles (INPRO) to help guide nuclear R and D strategies targeted on anticipated mid-century energy system needs. One part of INPRO seeks to develop cost targets for new designs to be competitive in mid-century markets. The starting point was the 40 scenarios of the Special Report on Emissions Scenarios (SRES) of the Intergovernmental Panel on Climate Change. This paper summarizes four of the SRES scenarios, one from each of the four SRES scenario families. It discusses their implications for nuclear energy, including cost targets, and develops for each an 'aggressive nuclear' variant. The aggressive nuclear variants estimate the potential market for nuclear energy if, by improving faster than assumed by the SRES authors, nuclear energy can make inroads into vulnerable market shares projected for its competitors. In addition to projected demands for nuclear generated electricity, hydrogen and heat, the aggressive variants include prospective demand for nuclear desalination and use in upgrading fossil fuels. The paper then presents learning rates and implied cost targets consistent with the aggressive nuclear variants of the SRES scenarios. One provocative initial result is that many of the scenarios with substantial nuclear expansion do not seem to require big reductions in nuclear investment costs. One interpretation discussed at the end of the paper highlights the difference between cost reductions consistent with long-term energy system optimization based on perfect foresight, and cost reductions necessary to attract private investment in today's 'deregulating' and uncertain energy markets. (orig.)

  12. Particle production from nuclear targets and the structure of hadrons

    International Nuclear Information System (INIS)

    Bialas, A.

    Production processes from nuclear targets allow studying interactions of elementary hadronic constituents in nuclear matter. The information thus obtained on the structure of hadrons and on the properties of hadronic constituents is presented. Both soft (low momentum transfer) and hard (high momentum transfer) processes are discussed. (author)

  13. Nuclear and radiation safety assurance federal target programme management system

    International Nuclear Information System (INIS)

    Kryukov, O.V.; Vasil'ev, V.A.; Nikishin, D.A.; Linge, I.I.; Obodinskij, A.N.

    2012-01-01

    The Federal Program Nuclear and Radiation Safety Assurance for 2008-2015 is presented. Specifics of Federal target program management as well as changes to program management are discussed. Data on evaluation of management effectiveness is given. Further efforts to resolve the nuclear legacy problem in Russia are also presented [ru

  14. EMC effect: asymptotic freedom with nuclear targets

    International Nuclear Information System (INIS)

    West, G.B.

    1984-01-01

    General features of the EMC effect are discussed within the framework of quantum chromodynamics as expressed via the operator product expansion and asymptotic freedom. These techniques are reviewed with emphasis on the target dependence. 22 references

  15. Thermal efficiency improvements - an imperative for nuclear generating stations

    International Nuclear Information System (INIS)

    Hassanien, S.; Rouse, S.

    1997-01-01

    A one and a half percent thermal performance improvement of Ontario Hydro's operating nuclear units (Bruce B, Pickering B, and Darlington) means almost 980 GWh are available to the transmission system (assuming an 80% capacity factor). This is equivalent to the energy consumption of 34,000 electrically-heated homes in Ontario, and worth more than $39 million in revenue to Ontario Hydro Nuclear Generation. Improving nuclear plant thermal efficiency improves profitability (more GWh per unit of fuel) and competitiveness (cost of unit energy), and reduces environmental impact (less spent fuel and nuclear waste). Thermal performance will naturally decrease due to the age of the units unless corrective action is taken. Most Ontario Hydro nuclear units are ten to twenty years old. Some common causes for loss of thermal efficiency are: fouling and tube plugging of steam generators, condensers, and heat exchangers; steam leaks in the condenser due to valve wear, steam trap and drain leaks; deposition, pitting, cracking, corrosion, etc., of turbine blades; inadequate feedwater metering resulting from corrosion and deposition. This paper stresses the importance of improving the nuclear units' thermal efficiency. Ontario Hydro Nuclear has demonstrated energy savings results are achievable and affordable. Between 1994 and 1996, Nuclear reduced its energy use and improved thermal efficiency by over 430,000 MWh. Efficiency improvement is not automatic - strategies are needed to be effective. This paper suggests practical strategies to systematically improve thermal efficiency. (author)

  16. Protein Targeting: ER Leads the Way to the Inner Nuclear Envelope.

    Science.gov (United States)

    Blackstone, Craig

    2017-12-04

    Efficient targeting of newly synthesized membrane proteins from the endoplasmic reticulum to the inner nuclear membrane depends on nucleotide hydrolysis. A new study shows that this dependence reflects critical actions of the atlastin family of GTPases in maintaining the morphology of the endoplasmic reticulum network. Published by Elsevier Ltd.

  17. Moving targets. Economic competitiveness of nuclear power

    International Nuclear Information System (INIS)

    Rogner, H.H.; Langlois, L.

    2000-01-01

    Most world electricity markets are now moving towards greater competition, driven in part by technology, low fuel prices, and experience that competitive markets are more self-sustaining. Electric power is being sold in a number of markets in member countries of the Organization for Economic Cooperation and Development (OECD) for around US $0.02 per kilowatt-hour (kWh). Can nuclear generation match such prices? If not, can it be made to do so? Electricity companies are now in the business of selling a commodity (kWh) and commercial services instead of a strategic good. Excess capacity, low demand growth and lower product prices in major industrialized countries have forced power generators and their suppliers to be more concerned with the costs of their operations and profitability of their investments. These companies increasingly need a commercial, profit-oriented approach if they are to survive and prosper. Even more, they will need to make substantial cost reductions over the next few years. The nuclear industry is no exception. How does nuclear power stack up in this environment? The IAEA Planning and Economic Studies Section is doing a series of studies on precisely these questions, divided into issues affecting the near, medium and long-term future of nuclear power. This corresponds roughly to matters affecting existing plants, upgrades and life extensions, or new plants. In general, the studies find that nuclear power has the potential to be competitive in all three markets. But realizing that potential will require significant changes on the part of the industry and its regulators. This article focuses on the prevailing market situation in many industrialized countries. Several lessons also are applicable to developing countries, particularly in cases where the financing of electric power projects is expected to come from international capital markets. The overall situation is distinctly different for developing countries. Typically the capacity there for

  18. Polarized nuclear target based on parahydrogen induced polarization

    OpenAIRE

    Budker, D.; Ledbetter, M. P.; Appelt, S.; Bouchard, L. S.; Wojtsekhowski, B.

    2012-01-01

    We discuss a novel concept of a polarized nuclear target for accelerator fixed-target scattering experiments, which is based on parahydrogen induced polarization (PHIP). One may be able to reach a 33% free-proton polarization in the ethane molecule. The potential advantages of such a target include operation at zero magnetic field, fast ($\\sim$100 Hz) polarization reversal, and operation with large intensity of an electron beam.

  19. Contemporary Targets for Control of Efficient Energy Use

    Directory of Open Access Journals (Sweden)

    Yu. S. Petrusha

    2012-01-01

    Full Text Available The paper describes main principles of the methodology for control of efficient energy use in power engineering and economy sectors as a whole. Targets for control of energy use at different levels have been considered in the paper. A special attention has been paid to technical, organizational and legal aspects of energy efficiency improvement. The paper contains an analysis of the history of the given issue, the present level of its development and near-term prospects under conditions of the Republic of Belarus.

  20. Energy Efficient and Reliable Target Monitoring in the Tactical Battlefield

    Science.gov (United States)

    Li, Yan-Xiao; Guan, Hua; Zhang, Yue-Ling

    In the tactical battlefield target monitoring it is crucial to take into account the energy efficiency and data reliability issues for the purpose of military decision making, especially in large scale sensor networks. However, due to the inherent nature of power constraint and wireless communication medium it is a challenging problem in the process of actual application. An efficient and reliable data aggregation scheme is proposed to enhance the performance of wireless sensor network used in the target monitoring. Firstly, the energy consumption model is presented and analyzed in the multihop WSNs. Then idea of mobile sinks, adaptive energy saving mechanism is introduced and the concept of multiple sinks cooperation is used to assure the reliability of the data aggregation. The simulation and the associated analysis show the improved results of the presented schema. At last the future discussion about the large scale tactical battlefield application is made to broaden the coming research scope.

  1. Pluronic F127 nanomicelles engineered with nuclear localized functionality for targeted drug delivery

    International Nuclear Information System (INIS)

    Li, Yong-Yong; Li, Lan; Dong, Hai-Qing; Cai, Xiao-Jun; Ren, Tian-Bin

    2013-01-01

    PKKKRKV (Pro-Lys-Lys-Lys-Arg-Lys-Val, PV7), a seven amino acid peptide, has emerged as one of the primary nuclear localization signals that can be targeted into cell nucleus via the nuclear import machinery. Taking advantage of chemical diversity and biological activities of this short peptide sequence, in this study, Pluronic F127 nanomicelles engineered with nuclear localized functionality were successfully developed for intracellular drug delivery. These nanomicelles with the size ∼ 100 nm were self-assembled from F127 polymer that was flanked with two PV7 sequences at its both terminal ends. Hydrophobic anticancer drug doxorubicin (DOX) with inherent fluorescence was chosen as the model drug, which was found to be efficiently encapsulated into nanomicelles with the encapsulation efficiency at 72.68%. In comparison with the non-functionalized namomicelles, the microscopic observation reveals that PV7 functionalized nanomicelles display a higher cellular uptake, especially into the nucleus of HepG2 cells, due to the nuclear localization signal effects. Both cytotoxicity and apoptosis studies show that the DOX-loaded nanomicelles were more potent than drug nanomicelles without nuclear targeting functionality. It was thus concluded that PV7 functionalized nanomicelles could be a potentially alternative vehicle for nuclear targeting drug delivery. - Highlights: ► A new nuclear targeted drug delivery system based on micelles is developed. ► This micellar system features a core-shell structure with the size peaked at 100 nm. ► PV7, a short peptide sequence, is adopted as a nuclear targeting ligand. ► PV7 functionalized drug loaded micelles are more potent in killing tumor cells

  2. DASPfind: new efficient method to predict drug–target interactions

    KAUST Repository

    Ba Alawi, Wail

    2016-03-16

    Background Identification of novel drug–target interactions (DTIs) is important for drug discovery. Experimental determination of such DTIs is costly and time consuming, hence it necessitates the development of efficient computational methods for the accurate prediction of potential DTIs. To-date, many computational methods have been proposed for this purpose, but they suffer the drawback of a high rate of false positive predictions. Results Here, we developed a novel computational DTI prediction method, DASPfind. DASPfind uses simple paths of particular lengths inferred from a graph that describes DTIs, similarities between drugs, and similarities between the protein targets of drugs. We show that on average, over the four gold standard DTI datasets, DASPfind significantly outperforms other existing methods when the single top-ranked predictions are considered, resulting in 46.17 % of these predictions being correct, and it achieves 49.22 % correct single top ranked predictions when the set of all DTIs for a single drug is tested. Furthermore, we demonstrate that our method is best suited for predicting DTIs in cases of drugs with no known targets or with few known targets. We also show the practical use of DASPfind by generating novel predictions for the Ion Channel dataset and validating them manually. Conclusions DASPfind is a computational method for finding reliable new interactions between drugs and proteins. We show over six different DTI datasets that DASPfind outperforms other state-of-the-art methods when the single top-ranked predictions are considered, or when a drug with no known targets or with few known targets is considered. We illustrate the usefulness and practicality of DASPfind by predicting novel DTIs for the Ion Channel dataset. The validated predictions suggest that DASPfind can be used as an efficient method to identify correct DTIs, thus reducing the cost of necessary experimental verifications in the process of drug discovery. DASPfind

  3. More efficient response to nuclear emergencies

    International Nuclear Information System (INIS)

    1979-12-01

    Data provided by the local authorities in the counties in which the Oskarshamn and Barsebaeck nuclear power plants are situated is presented. The data is for planning of evaluation in the case of a reactor accident and includes population, population distribution, age distribution, institutions such as schools and hospitals, transport, both public and private and accommodation possibilities. Agricultural and domestic animal data are also provided. (J.I.W.)

  4. Nuclear targets within the project of solving CHAllenges in Nuclear DAta

    Science.gov (United States)

    Sibbens, Goedele; Moens, André; Vanleeuw, David; Lewis, David; Aregbe, Yetunde

    2017-09-01

    In the frame of the European Commission funded integrated project CHANDA (solving CHAllenges in Nuclear DAta) the importance of nuclear target preparation for the accurateness and reliability of experimental nuclear data is set in a dedicated work package (WP3). The global aim of WP3 is the development of a network for nuclear target preparation and characterization, enabling to coordinate the target production corresponding to the experimental requirements. Therefore, a set of tasks within the work package needs to be followed. Primarily, an inventory of target related facilities and radioisotope providers was created. In the next step a priority list of target requests was made in agreement with the target user considering the technical specification, the scheduled experiments and the availability of the target laboratories. A set of target requests has been assigned to the Target Preparation laboratory of the European Commission - Joint Research Centre - Directorate G (EC-JRC.G.2) in Geel, Belgium. This contribution gives an overview of the nuclear targets that are produced within the CHANDA project. The equipment and techniques available for the preparation and characterization of uranium, plutonium and neptunium layers with an areal density ranging from 60 to 205 μg cm-2 will be emphasized.

  5. Practical methods of target preparation for use in nuclear experiments

    International Nuclear Information System (INIS)

    Sugai, Isao.

    1976-01-01

    This is the fifth report on the practical methods of target preparation for use in nuclear experiments following the previous one (INS-J-152, 1975). Electro-deposition is a very powerful technique well suited to the preparation of self-supporting targets of Ni, Cr, Zn, Rh, Cd, Sb and Pb metals over a wide range of thickness from 1 to 20 mg/cm 2 . The uniformities of the thicknesses of Cr, Zn, Rh, Cd and Pb targets were measured with α- and β-ray thickness gauges. The impurities in Cr target were checked by the measurement of elastically scattered protons, and by a optical spectrometer. (auth.)

  6. Targeting an efficient target-to-target interval for P300 speller brain–computer interfaces

    Science.gov (United States)

    Sellers, Eric W.; Wang, Xingyu

    2013-01-01

    Longer target-to-target intervals (TTI) produce greater P300 event-related potential amplitude, which can increase brain–computer interface (BCI) classification accuracy and decrease the number of flashes needed for accurate character classification. However, longer TTIs requires more time for each trial, which will decrease the information transfer rate of BCI. In this paper, a P300 BCI using a 7 × 12 matrix explored new flash patterns (16-, 18- and 21-flash pattern) with different TTIs to assess the effects of TTI on P300 BCI performance. The new flash patterns were designed to minimize TTI, decrease repetition blindness, and examine the temporal relationship between each flash of a given stimulus by placing a minimum of one (16-flash pattern), two (18-flash pattern), or three (21-flash pattern) non-target flashes between each target flashes. Online results showed that the 16-flash pattern yielded the lowest classification accuracy among the three patterns. The results also showed that the 18-flash pattern provides a significantly higher information transfer rate (ITR) than the 21-flash pattern; both patterns provide high ITR and high accuracy for all subjects. PMID:22350331

  7. Nuclear target foil fabrication for the Romano Event

    International Nuclear Information System (INIS)

    Weed, J.W.; Romo, J.G. Jr.; Griggs, G.E.

    1984-01-01

    The Vacuum Processes Lab, of LLNL's M.E. Dept. - Material Fabrication Division, was requested to provide 250 coated Parylene target foils for a nuclear physics experiment titled the ROMANO Event. Due to the developmental nature of some of the fabrication procedures, approximately 400 coated foils were produced to satisfy the event's needs. The foils were used in the experiment as subkilovolt x-ray, narrow band pass filters, and wide band ultraviolet filters. This paper is divided into three sections describing: (1) nuclear target foil fabrication, (2) Parylene substrate preparation and production, and (3) foil and substrate inspections

  8. Nuclear materials management for safety and efficiency

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1965-12-15

    The use of nuclear materials in industrial processes presents management with some special problems which are peculiar to the atomic energy industry. If reactor fuel costs are to be kept low, too, each fuel element must yield the maximum economic 'bum-up' before it is withdrawn from service, and this calls for reliable non-destructive methods of measurement of 'burn-up' and appropriate records and fuel-changing schedules. The special hazards of radioactive materials call for special precautions and appropriate systems of handling and storage. A further danger unique to atomic energy is that of criticality - the possibility that an excessive concentration of fissile material may result in a chain reaction. Every part of the processing plant must be surveyed and checked to ensure that there is no build-up of fissile residues; in storage or transit there must be no aggregation of small lots. In the nuclear energy industry, too, the standards of purity required are much higher than in most other large-scale operation, so that stringent quality checks are needed

  9. Efficient energy-saving targets for APEC economies

    International Nuclear Information System (INIS)

    Hu, J.-L.; Kao, C.-H.

    2007-01-01

    Energy-saving target ratios (ESTR) for 17 APEC economies during 1991-2000 are computed in a total-factor framework. All nominal variables are transformed into real variables by the purchasing power parity (PPP) at the 1995 price level. The data envelopment analysis (DEA) approach is used to find the energy-saving target (EST) for APEC economies without reducing their maximum potential gross domestic productions (GDPs) in each year. Energy, labor, and capital are the three inputs, while GDP is the single output. Our major findings are as follows: (1) China has the largest EST up to almost half of its current usage. (2) Hong Kong, the Philippines, and the United States have the highest energy efficiency. (3) The energy efficiency generally increases for APEC economies except for Canada and New Zealand. (4) Chile, Mexico, and Taiwan have significantly improved their energy efficiency in the last 5 years. (5) An inverted U-shape relation exists between per capita EST and per capita GDP. (6) ESTR has a positive relation with the value-added percentage of GDP of the industry sector and a negative relation with that of the service sector

  10. Deposition techniques for the preparation of thin film nuclear targets

    International Nuclear Information System (INIS)

    Muggleton, A.H.F.

    1987-07-01

    This review commences with a brief description of the basic principles that regulate vacuum evaporation and the physical processes involved in thin film formation, followed by a description of the experimental methods used. The principle methods of heating the evaporant are detailed and the means of measuring and controlling the film thickness are elucidated. Types of thin film nuclear targets are considered and various film release agents are listed. Thin film nuclear target behaviour under ion-bombardment is described and the dependence of nuclear experimental results upon target thickness and uniformity is outlined. Special problems associated with preparing suitable targets for lifetime measurements are discussed. The causes of stripper-foil thickening and breaking under heavy-ion bombardment are considered. A comparison is made between foils manufactured by a glow discharge process and those produced by vacuum sublimation. Consideration is given to the methods of carbon stripper-foil manufacture and to the characteristics of stripper-foil lifetimes are considered. Techniques are described that have been developed for the fabrication of special targets, both from natural and isotopically enriched material, and also of elements that are either chemically unstable, or thermally unstable under irradiation. The reduction of metal oxides by the use of hydrogen or by utilising a metallothermic technique, and the simultaneous evaporation of reduced rare earth elements is described. A comprehensive list of the common targets is presented

  11. DASPfind: new efficient method to predict drug–target interactions

    KAUST Repository

    Ba Alawi, Wail; Soufan, Othman; Essack, Magbubah; Kalnis, Panos; Bajic, Vladimir B.

    2016-01-01

    DASPfind is a computational method for finding reliable new interactions between drugs and proteins. We show over six different DTI datasets that DASPfind outperforms other state-of-the-art methods when the single top-ranked predictions are considered, or when a drug with no known targets or with few known targets is considered. We illustrate the usefulness and practicality of DASPfind by predicting novel DTIs for the Ion Channel dataset. The validated predictions suggest that DASPfind can be used as an efficient method to identify correct DTIs, thus reducing the cost of necessary experimental verifications in the process of drug discovery. DASPfind can be accessed online at: http://​www.​cbrc.​kaust.​edu.​sa/​daspfind.

  12. Efficient visualization of high-throughput targeted proteomics experiments: TAPIR.

    Science.gov (United States)

    Röst, Hannes L; Rosenberger, George; Aebersold, Ruedi; Malmström, Lars

    2015-07-15

    Targeted mass spectrometry comprises a set of powerful methods to obtain accurate and consistent protein quantification in complex samples. To fully exploit these techniques, a cross-platform and open-source software stack based on standardized data exchange formats is required. We present TAPIR, a fast and efficient Python visualization software for chromatograms and peaks identified in targeted proteomics experiments. The input formats are open, community-driven standardized data formats (mzML for raw data storage and TraML encoding the hierarchical relationships between transitions, peptides and proteins). TAPIR is scalable to proteome-wide targeted proteomics studies (as enabled by SWATH-MS), allowing researchers to visualize high-throughput datasets. The framework integrates well with existing automated analysis pipelines and can be extended beyond targeted proteomics to other types of analyses. TAPIR is available for all computing platforms under the 3-clause BSD license at https://github.com/msproteomicstools/msproteomicstools. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  13. Nuclear reactions of high energy deuterons with medium mass targets

    International Nuclear Information System (INIS)

    Numajiri, Masaharu; Miura, Taichi; Oki, Yuichi

    1994-01-01

    Formation cross sections of product nuclides in the nuclear reactions of medium mass targets by 10 GeV deuterons were measured with a gamma-ray spectroscopy. The measured data were compared with the cross sections of 12 GeV protons. (author)

  14. Nuclear Data Target Accuracy Requirements For MA Burners

    International Nuclear Information System (INIS)

    Palmiotti, G.; Salvatores, M.

    2011-01-01

    A nuclear data target accuracy assessment has been carried out for two types of transmuters: a critical sodium fast reactor(SFR) and an accelerator driven system (ADMAB). Results are provided for a 7 group energy structure. Considerations about fuel cycle parameters uncertainties illustrate their dependence from the isotope final densities at end of cycle.

  15. Energy efficiency improvement target for SIC 34 - fabricated metal products. Revised target support document

    Energy Technology Data Exchange (ETDEWEB)

    Byrer, T. G.; Billhardt, C. F.; Farkas, M. S.

    1977-02-15

    In accordance with section 374 of the Energy Policy and Conservation Act (EPCA), Pub. L. 94-163, the Federal Energy Administration (FEA) proposed industrial energy efficiency improvement targets for the ten most energy-consumptive manufacturing industries in the U.S. Following public hearings and a review of the comments made, the final targets for Fabricated Metal Products (SIC 34) were established and are described. Using 1972 data on the energy consumed to produce specific metal products, it was concluded that a 24% reduction in energy consumption for SIC 34 is a viable goal for achievement by 1980. (ERA citation 04:045006)

  16. Energy efficient hotspot-targeted embedded liquid cooling of electronics

    International Nuclear Information System (INIS)

    Sharma, Chander Shekhar; Tiwari, Manish K.; Zimmermann, Severin; Brunschwiler, Thomas; Schlottig, Gerd; Michel, Bruno; Poulikakos, Dimos

    2015-01-01

    Highlights: • We present a novel concept for hotspot-targeted, energy efficient ELC for electronic chips. • Microchannel throttling zones distribute flow optimally without any external control. • Design is optimized for highly non-uniform multicore chip heat flux maps. • Optimized design minimizes chip temperature non-uniformity. • This is achieved with pumping power consumption less than 1% of total chip power. - Abstract: Large data centers today already account for nearly 1.31% of total electricity consumption with cooling responsible for roughly 33% of that energy consumption. This energy intensive cooling problem is exacerbated by the presence of hotspots in multicore microprocessors due to excess coolant flow requirement for thermal management. Here we present a novel liquid-cooling concept, for targeted, energy efficient cooling of hotspots through passively optimized microchannel structures etched into the backside of a chip (embedded liquid cooling or ELC architecture). We adopt an experimentally validated and computationally efficient modeling approach to predict the performance of our hotspot-targeted ELC design. The design is optimized for exemplar non-uniform chip power maps using Response Surface Methodology (RSM). For industrially acceptable limits of approximately 0.4 bar (40 kPa) on pressure drop and one percent of total chip power on pumping power, the optimized designs are computationally evaluated against a base, standard ELC design with uniform channel widths and uniform flow distribution. For an average steady-state heat flux of 150 W/cm 2 in core areas (hotspots) and 20 W/cm 2 over remaining chip area (background), the optimized design reduces the maximum chip temperature non-uniformity by 61% to 3.7 °C. For a higher average, steady-state hotspot heat flux of 300 W/cm 2 , the maximum temperature non-uniformity is reduced by 54% to 8.7 °C. It is shown that the base design requires a prohibitively high level of pumping power (about

  17. Nuclear Waste Vitrification Efficiency: Cold Cap Reactions

    International Nuclear Information System (INIS)

    Kruger, A.A.; Hrma, P.R.; Pokorny, R.

    2011-01-01

    The cost and schedule of nuclear waste treatment and immobilization are greatly affected by the rate of glass production. Various factors influence the performance of a waste-glass melter. One of the most significant, and also one of the least understood, is the process of batch melting. Studies are being conducted to gain fundamental understanding of the batch reactions, particularly those that influence the rate of melting, and models are being developed to link batch makeup and melter operation to the melting rate. Batch melting takes place within the cold cap, i.e., a batch layer floating on the surface of molten glass. The conversion of batch to glass consists of various chemical reactions, phase transitions, and diffusion-controlled processes. These include water evaporation (slurry feed contains as high as 60% water), gas evolution, the melting of salts, the formation of borate melt, reactions of borate melt with molten salts and with amorphous oxides (Fe 2 O 3 and Al 2 O 3 ), the formation of intermediate crystalline phases, the formation of a continuous glass-forming melt, the growth and collapse of primary foam, and the dissolution of residual solids. To this list we also need to add the formation of secondary foam that originates from molten glass but accumulates on the bottom of the cold cap. This study presents relevant data obtained for a high-level-waste melter feed and introduces a one-dimensional (1D) mathematical model of the cold cap as a step toward an advanced three-dimensional (3D) version for a complete model of the waste glass melter. The 1D model describes the batch-to-glass conversion within the cold cap as it progresses in a vertical direction. With constitutive equations and key parameters based on measured data, and simplified boundary conditions on the cold-cap interfaces with the glass melt and the plenum space of the melter, the model provides sensitivity analysis of the response of the cold cap to the batch makeup and melter

  18. Scintillation efficiency of nuclear recoil in liquid xenon

    CERN Document Server

    Arneodo, F; Badertscher, A; Benetti, P; Bernardini, E; Bettini, A; Borio di Tigliole, A A; Brunetti, R; Bueno, A G; Calligarich, E; Campanelli, M; Carpanese, C; Cavalli, D; Cavanna, F; Cennini, P; Centro, Sandro; Cesana, A; Cline, D; De Mitri, I; Dolfini, R; Ferrari, A; Gigli-Berzolari, A; Matthey, C; Mauri, F; Mazza, D; Mazzone, L; Meng, G; Montanari, C; Nurzia, G; Otwinowski, S; Palamara, O; Pascoli, D; Pepato, Adriano; Petrera, S; Periale, L; Piano Mortari, G; Piazzoli, A; Picchi, P; Pietropaolo, F; Rancati, T; Rappoldi, A; Raselli, G L; Rebuzzi, D; Revol, Jean Pierre Charles; Rico, J; Rossella, M; Rossi, C; Rubbia, André; Rubbia, Carlo; Sala, P; Scannicchio, D A; Sergiampietri, F; Suzuki, S; Terrani, M; Tian, W; Ventura, Sandro; Vignoli, C; Wang, H; Woo, J; Xu, Z

    2000-01-01

    We present the results of a test done with a Liquid Xenon (LXe) detector for 'Dark Matter' search, exposed to a neutron beam to produce nuclear recoil events simulating those which would be generated by WIMP's elastic scattering. The aim of the experiment was to measure directly the scintillation efficiency of nuclear recoil. The nuclear recoil considered in the test was in the tens of keV range. The ratio of measured visible energy over the true recoil energy was evaluated to be about 20%, in good agreement with the theoretical predictions.

  19. Reviews on the efficient nuclear material accountability at KAERI

    International Nuclear Information System (INIS)

    Kim, Hyun Sook; Ko, Han Suk; Lee, Seung Ho; Park, Ho Joon; Lee, Byung Doo

    2011-01-01

    KAERI(Korea Atomic Energy Research Institute) is constantly trying to efficiently implement safeguards in order to ensure international transparency and the credibility of KAERI safeguards. In its continuing efforts to implement safeguards efficiently, KAERI has developed KASIS(KAERI Safeguards Information Treatment System) and has linked KASIS with the RF ID system and HANARO fuel fabrication facility system. This paper describes the status of enhanced nuclear material accountancy through the development of KASIS at KAERI. The plans to more effectively implement nuclear material accountancy at KAERI are also reviewed

  20. An efficient targeted drug delivery through apotransferrin loaded nanoparticles.

    Directory of Open Access Journals (Sweden)

    Athuluri Divakar Sai Krishna

    Full Text Available BACKGROUND: Cancerous state is a highly stimulated environment of metabolically active cells. The cells under these conditions over express selective receptors for assimilation of factors essential for growth and transformation. Such receptors would serve as potential targets for the specific ligand mediated transport of pharmaceutically active molecules. The present study demonstrates the specificity and efficacy of protein nanoparticle of apotransferrin for targeted delivery of doxorubicin. METHODOLOGY/PRINCIPAL FINDINGS: Apotransferrin nanoparticles were developed by sol-oil chemistry. A comparative analysis of efficiency of drug delivery in conjugated and non-conjugated forms of doxorubicin to apotransferrin nanoparticle is presented. The spherical shaped apotransferrin nanoparticles (nano have diameters of 25-50 etam, which increase to 60-80 etam upon direct loading of drug (direct-nano, and showed further increase in dimension (75-95 etam in conjugated nanoparticles (conj-nano. The competitive experiments with the transferrin receptor specific antibody showed the entry of both conj-nano and direct-nano into the cells through transferrin receptor mediated endocytosis. Results of various studies conducted clearly establish the superiority of the direct-nano over conj-nano viz. (a localization studies showed complete release of drug very early, even as early as 30 min after treatment, with the drug localizing in the target organelle (nucleus (b pharmacokinetic studies showed enhanced drug concentrations, in circulation with sustainable half-life (c the studies also demonstrated efficient drug delivery, and an enhanced inhibition of proliferation in cancer cells. Tissue distribution analysis showed intravenous administration of direct nano lead to higher drug localization in liver, and blood as compared to relatively lesser localization in heart, kidney and spleen. Experiments using rat cancer model confirmed the efficacy of the formulation in

  1. The Jet Experiments in Nuclear Structure and Astrophysics (JENSA) gas jet target

    International Nuclear Information System (INIS)

    Chipps, K.A.; Greife, U.; Bardayan, D.W.; Blackmon, J.C.; Kontos, A.; Linhardt, L.E.; Matos, M.; Pain, S.D.; Pittman, S.T.; Sachs, A.; Schatz, H.; Schmitt, K.T.; Smith, M.S.; Thompson, P.

    2014-01-01

    New radioactive ion beam (RIB) facilities will push further away from stability and enable the next generation of nuclear physics experiments. Of great importance to the future of RIB physics are scattering, transfer, and capture reaction measurements of rare, exotic, and unstable nuclei on light targets such as hydrogen and helium. These measurements require targets that are dense, highly localized, and pure. Targets must also accommodate the use of large area silicon detector arrays, high-efficiency gamma arrays, and heavy ion detector systems to efficiently measure the reaction products. To address these issues, the Jet Experiments in Nuclear Structure and Astrophysics (JENSA) Collaboration has designed, built, and characterized a supersonic gas jet target, capable of providing gas areal densities on par with commonly used solid targets within a region of a few millimeters diameter. Densities of over 5×10 18 atoms/cm 2 of helium have been achieved, making the JENSA gas jet target the most dense helium jet achieved so far

  2. Fabrication of 121Sb isotopic targets for the study of nuclear high spin features

    Science.gov (United States)

    Devi, K. Rojeeta; Kumar, Suresh; Kumar, Neeraj; Abhilash, S. R.; Kabiraj, D.

    2018-06-01

    Isotopic 121Sb targets with 197Au backing have been prepared by Physical Vapor Deposition (PVD) method using the diffusion pump based coating unit at target laboratory, Inter University Accelerator Centre (IUAC), New Delhi, India. The target thickness was measured by stylus profilo-meter and the purity of the targets was investigated by Energy Dispersive X-ray Analysis (EDXA). One of these targets has been used in an experiment which was performed at IUAC for nuclear structure study through fusion evaporation reaction. The excitation function of the 121Sb(12C, yxnγ) reaction has been performed for energies 58 to 70 MeV in steps of 4 MeV. The experimental results were compared with the calculations of statistical models : PACE4 and CASCADE. The methods adopted to achieve best quality foils and good deposition efficiency are reported in this paper.

  3. A nuclear standard high-efficiency adsorber for iodine

    International Nuclear Information System (INIS)

    Wang Jianmin; Qian Yinge

    1988-08-01

    The structure of a nuclear standard high-efficiency adsorber, adsorbent and its performance are introduced. The performance and structure were compared with the same kind product of other firms. The results show that the leakage rate is less than 0.005%

  4. Isotopic characterization of targets for nuclear measurements at CBNM

    International Nuclear Information System (INIS)

    Bievre, P. de

    1985-01-01

    Nuclear measurements for which ''nuclear'' targets are prepared are almost always isotope-specific i.e. they are normally related to a particular nuclide in the target. The amount of this nuclide must be accurately assessed. There are essentially two ways to determine the number of atoms of this particular nuclide. (1) By determination of the amount of element, to which the nuclide belongs, on the target via classsical means; weighing substraction of impurities, calculation of element amount using known of the chemical compound in which the element is incorporated and, finally, measurement of the isotopic composition in order to determine the fraction of the nuclide concerned in the element. An alternative way may be to perform an elemental assay on the target followed by determination of the isotopic composition. (2) Another approach is isotope dilution mass spectrometry where a change in the isotopic composition of the ''target'' is induced by adding a known number of atoms (called ''spike'') of the element with a quite different composition. Measurement of the resulting change in isotopic composition yields directly the number of atoms of the nuclide under investigation. The method is highly selective, accurate and isotope-specific. (orig.)

  5. Laser-driven nuclear-polarized hydrogen internal gas target

    International Nuclear Information System (INIS)

    Seely, J.; Crawford, C.; Clasie, B.; Xu, W.; Dutta, D.; Gao, H.

    2006-01-01

    We report the performance of a laser-driven polarized internal hydrogen gas target (LDT) in a configuration similar to that used in scattering experiments. This target used the technique of spin-exchange optical pumping to produce nuclear spin polarized hydrogen gas that was fed into a cylindrical storage (target) cell. We present in this paper the performance of the target, methods that were tried to improve the figure-of-merit (FOM) of the target, and a Monte Carlo simulation of spin-exchange optical pumping. The dimensions of the apparatus were optimized using the simulation and the experimental results were in good agreement with the results from the simulation. The best experimental result achieved was at a hydrogen flow rate of 1.1x10 18 atoms/s, where the sample beam exiting the storage cell had 58.2% degree of dissociation and 50.5% polarization. Based on this measurement, the atomic fraction in the storage cell was 49.6% and the density averaged nuclear polarization was 25.0%. This represents the highest FOM for hydrogen from an LDT and is higher than the best FOM reported by atomic beam sources that used storage cells

  6. Galactosylated DNA lipid nanocapsules for efficient hepatocyte targeting.

    Science.gov (United States)

    Morille, M; Passirani, C; Letrou-Bonneval, E; Benoit, J-P; Pitard, B

    2009-09-11

    The main objective of gene therapy via a systemic pathway is the development of a stable and non-toxic gene vector that can encapsulate and deliver foreign genetic materials into specific cell types with the transfection efficiency of viral vectors. With this objective, DNA complexed with cationic lipids of DOTAP/DOPE was encapsulated into lipid nanocapsules (LNCs) forming nanocarriers (DNA LNCs) with a size suitable for systemic injection (109+/-6 nm). With the goal of increasing systemic delivery, LNCs were stabilised with long chains of poly(ethylene glycol) (PEG), either from a PEG lipid derivative (DSPE-mPEG(2000)) or from an amphiphilic block copolymer (F108). In order to overcome internalisation difficulties encountered with PEG shield, a specific ligand (galactose) was covalently added at the distal end of the PEG chains, in order to provide active targeting of the asialoglycoprotein-receptor present on hepatocytes. This study showed that DNA LNCs were as efficient as positively charged DOTAP/DOPE lipoplexes for transfection. In primary hepatocytes, when non-galactosylated, the two polymers significantly decreased the transfection, probably by creating a barrier around the DNA LNCs. Interestingly, galactosylated F108 coated DNA LNCs led to a 18-fold increase in luciferase expression compared to non-galactosylated ones.

  7. Actinide targets for fundamental research in nuclear physics

    Science.gov (United States)

    Eberhardt, K.; Düllmann, Ch. E.; Haas, R.; Mokry, Ch.; Runke, J.; Thörle-Pospiech, P.; Trautmann, N.

    2018-05-01

    Thin actinide layers deposited on various substrates are widely used as calibration sources in nuclear spectroscopy. Other applications include fundamental research in nuclear chemistry and -physics, e.g., the chemical and physical properties of super-heavy elements (SHE, Z > 103) or nuclear reaction studies with heavy ions. For the design of future nuclear reactors like fast-fission reactors and accelerator-driven systems for transmutation of nuclear waste, precise data for neutron absorption as well as neutron-induced fission cross section data for 242Pu with neutrons of different energies are of particular importance, requiring suitable Pu-targets. Another application includes studies of nuclear transitions in 229Th harvested as α-decay recoil product from a thin layer of its 233U precursor. For this, a thin and very smooth layer of 233U is used. We report here on the production of actinide layers mostly obtained by Molecular Plating (MP). MP is currently the only fabrication method in cases where the desired actinide material is available only in very limited amounts or possesses a high specific activity. Here, deposition is performed from organic solution applying a current density of 1-2 mA/cm2. Under these conditions target thicknesses of 500-1000 μg/cm2 are possible applying a single deposition step with deposition yields approaching 100 %. For yield determination α-particle spectroscopy, γ-spectroscopy and Neutron Activation Analysis is routinely used. Layer homogeneity is checked with Radiographic Imaging. As an alternative technique to MP the production of thin lanthanide and actinide layers by the so-called "Drop on Demand"-technique applied e.g., in ink-jet printing is currently under investigation.

  8. Nuclear EGFR as a molecular target in cancer

    International Nuclear Information System (INIS)

    Brand, Toni M.; Iida, Mari; Luthar, Neha; Starr, Megan M.; Huppert, Evan J.; Wheeler, Deric L.

    2013-01-01

    The epidermal growth factor receptor (EGFR) has been one of the most targeted receptors in the field of oncology. While anti-EGFR inhibitors have demonstrated clinical success in specific cancers, most patients demonstrate either intrinsic or acquired resistance within one year of treatment. Many mechanisms of resistance to EGFR inhibitors have been identified, one of these being attributed to alternatively localized EGFR from the cell membrane into the cell’s nucleus. Inside the nucleus, EGFR functions as a co-transcription factor for several genes involved in cell proliferation and angiogenesis, and as a tyrosine kinase to activate and stabilize proliferating cell nuclear antigen and DNA dependent protein kinase. Nuclear localized EGFR is highly associated with disease progression, worse overall survival in numerous cancers, and enhanced resistance to radiation, chemotherapy, and the anti-EGFR therapies gefitinib and cetuximab. In this review the current knowledge of how nuclear EGFR enhances resistance to cancer therapeutics is discussed, in addition to highlighting ways to target nuclear EGFR as an anti-cancer strategy in the future

  9. The influence of target properties on nuclear spectroscopy measurements

    International Nuclear Information System (INIS)

    Dionisio, J.S.; Vieu, C.; Lagrange, J.M.; Pautrat, M.; Vanhorenbeeck, J.; Passoja, A.

    1988-01-01

    A broad review of different kinds of in-beam nuclear spectroscopy measurements particularly influenced by the target properties is outlined. To illustrate such an influence a few typical examples of in-beam electron and gamma-ray spectroscopy measurements, performed at the Orsay MP Tandem accelerator, are reported. In particular several applications of the recoil ion catcher method in the study of short-lived nuclear isomers (with half-lives between ten and few hundred nanoseconds) are briefly described. This method is operated mostly with a pulsed heavy ion beam, bombarding a thin self-supported target but avoiding hitting the catcher foil. Moreover, the time of flight filtering properties of this experimental device is improved by a fast detection of compound nucleus deexcitation (performed with an array of several BaF 2 crystals). This kind of measurement shows clearly the importance of the target qualities as well as the need of good focusing properties and time structure for the accelerated particle beam. Finally, the required characteristics of the targets and recoil stopper foils needed for these measurements (and similar ones performed with the recoil ion shadow method) are analyzed in detail for a few typical experimental arrangements. (author). Abstract only

  10. Target-fueled nuclear reactor for medical isotope production

    Science.gov (United States)

    Coats, Richard L.; Parma, Edward J.

    2017-06-27

    A small, low-enriched, passively safe, low-power nuclear reactor comprises a core of target and fuel pins that can be processed to produce the medical isotope .sup.99Mo and other fission product isotopes. The fuel for the reactor and the targets for the .sup.99Mo production are the same. The fuel can be low enriched uranium oxide, enriched to less than 20% .sup.235U. The reactor power level can be 1 to 2 MW. The reactor is passively safe and maintains negative reactivity coefficients. The total radionuclide inventory in the reactor core is minimized since the fuel/target pins are removed and processed after 7 to 21 days.

  11. Mitochondrial Spare Respiratory Capacity Is Negatively Correlated with Nuclear Reprogramming Efficiency

    DEFF Research Database (Denmark)

    Yan, Zhou; Al-Saaidi, Rasha Abdelkadhem; Fernandez Guerra, Paula

    2017-01-01

    Nuclear reprogramming efficiency has been shown to be highly variable among different types of somatic cells and different individuals, yet the underlying mechanism remains largely unknown. Several studies have shown that reprogramming of fibroblasts into induced pluripotent stem cells (i......, opposed to fibroblasts with the highest mitochondrial SRC, which showed lowest reprogramming efficiency. Furthermore, we found that targeted fluorescent tagging of endogenous genes (MYH6 and COL2A1) by CRISPR/Cas9-mediated homologous recombination was accompanied by an increase in the SRC level...

  12. Mitochondrial Spare Respiratory Capacity Is Negatively Correlated With Nuclear Reprogramming Efficiency

    DEFF Research Database (Denmark)

    Zhou, Yan; Al-Saaidi, Rasha Abdelkadhem; Guerra, Paula Fernandez

    2017-01-01

    Nuclear reprogramming efficiency has been shown to be highly variable among different types of somatic cells and different individuals, yet the underlying mechanism remains largely unknown. Several studies have shown that reprogramming of fibroblasts into induced pluripotent stem cells (i......, opposed to fibroblasts with the highest mitochondrial SRC, which showed lowest reprogramming efficiency. Furthermore, we found that targeted fluorescent tagging of endogenous genes (MYH6 and COL2A1) by CRISPR/Cas9-mediated homologous recombination was accompanied by an increase in the SRC level...

  13. Survey of numerical safety targets for nuclear power plants

    International Nuclear Information System (INIS)

    Kelley, A.P. Jr.; Buttemer, D.R.

    1981-04-01

    The construction of a nuclear power plant implies, as does the construction of any major public work, the acceptance of a finite degree of risk. This risk can be reduced by an increased investment in engineered safeguards. However, at some level of risk, overinvestment in safety can render the project uneconomical. Because of the desirability of fixing safety standards on an absolute basis, there has long been an interest in establishing numerical risk criteria for the design, construction, and operation of nuclear power plants. Interest in the subject of numerical safety goals has recently been intensified by the Three Mile Island Action Plan. The USNRC has been directed by Congress to develop a national safety goal for reactor regulation. This report summarizes actions which have been historically, and are currently, taking place toward establishing national numerical risk targets for reactor regulation. Emphasis is placed upon actions taken, or currently being taken, by federal regulatory agencies and directly associated advisory bodies

  14. High-spin nuclear target of 178m2Hf: creation and nuclear reaction studies

    International Nuclear Information System (INIS)

    Oganessyan, Yu.Ts.; Karamyan, S.A.; Gangrskij, Yu.P.

    1993-01-01

    A long-lived (31 years) four-quasiparticle isomer 178m 2 Hf(I,K π =16,16 + ) was produced in microweight quantities using the nuclear reaction 176 Yb( 4 He, 2n). Methods of precision chemistry and mass-separation for the purification of the produced Hf material have been developed. Thin targets of isomeric hafnium-178 on carbon backings were prepared and used in experiments on a neutron, proton and deuteron beams. First results on nuclear reactions on a high-spin exotic target were obtained. Experiments on electromagnetic interactions of the isomeric hafnium using methods of the collinear laser spectroscopy as well as of the nuclear orientation of hafnium implanted into a crystalline media were started. 11 refs.; 11 figs.; 2 tabs

  15. HIGH EFFICIENCY GENERATION OF HYDROGEN FUELS USING NUCLEAR POWER

    Energy Technology Data Exchange (ETDEWEB)

    BROWN,LC; BESENBRUCH,GE; LENTSCH,RD; SCHULTZ,KR; FUNK,JF; PICKARD,PS; MARSHALL,AC; SHOWALTER,SK

    2003-06-01

    OAK B202 HIGH EFFICIENCY GENERATION OF HYDROGEN FUELS USING NUCLEAR POWER. Combustion of fossil fuels, used to power transportation, generate electricity, heat homes and fuel industry provides 86% of the world's energy. Drawbacks to fossil fuel utilization include limited supply, pollution, and carbon dioxide emissions. Carbon dioxide emissions, thought to be responsible for global warming, are now the subject of international treaties. Together, these drawbacks argue for the replacement of fossil fuels with a less-polluting potentially renewable primary energy such as nuclear energy. Conventional nuclear plants readily generate electric power but fossil fuels are firmly entrenched in the transportation sector. Hydrogen is an environmentally attractive transportation fuel that has the potential to displace fossil fuels. Hydrogen will be particularly advantageous when coupled with fuel cells. Fuel cells have higher efficiency than conventional battery/internal combustion engine combinations and do not produce nitrogen oxides during low-temperature operation. Contemporary hydrogen production is primarily based on fossil fuels and most specifically on natural gas. When hydrogen is produced using energy derived from fossil fuels, there is little or no environmental advantage. There is currently no large scale, cost-effective, environmentally attractive hydrogen production process available for commercialization, nor has such a process been identified. The objective of this work is to find an economically feasible process for the production of hydrogen, by nuclear means, using an advanced high-temperature nuclear reactor as the primary energy source. Hydrogen production by thermochemical water-splitting (Appendix A), a chemical process that accomplishes the decomposition of water into hydrogen and oxygen using only heat or, in the case of a hybrid thermochemical process, by a combination of heat and electrolysis, could meet these goals. Hydrogen produced from

  16. Gas, Liquid and Molten Targets at Cyclotron Beams: Target Systems and Related Nuclear Database

    Energy Technology Data Exchange (ETDEWEB)

    Ditrói, F.; Tárkányi, F.; Takács, S. [Institute of Nuclear Research of the Hungarian Academy of Sciences (ATOMKI), Debrecen (Hungary)

    2009-07-01

    In a systematic study our group worked on measurement of activation data of charged particle induced nuclear reaction possible involved in the production of radioisotopes for medical use. We have investigated the cross section and yield data of proton, deuteron helium-3 and alpha particle induced reactions on target materials for isotope productions relevant for medical applications as well as nuclear reactions on different structural material applied in construction of different target units. The acquired information are used or in the future can be used to determine the type of the construction materials optimal for building a certain target unit to be able to produce high specific activity and high quality radioactive isotope. In this work we have investigated different materials in activation point of view. Also several materials and reactions were studied for monitoring purposes. Use of thin metallic foils is a simple method to determine the parameters of the charged particle beams applied for isotope production. Using this method one can determine the energy and intensity of the bombarding beam and in certain cases the distribution or the profile of the bombarding beam. (author)

  17. Simultaneous nuclear data target accuracy study for innovative fast reactors

    International Nuclear Information System (INIS)

    Aliberti, G.; Palmiotti, G.; Salvatores, M.

    2007-01-01

    The present paper summarizes the major outcomes of a study conducted within a Nuclear Energy Agency Working Party on Evaluation Cooperation (NEA WPEC) initiative aiming to investigate data needs for future innovative nuclear systems, to quantify them and to propose a strategy to meet them. Within the NEA WPEC Subgroup 26 an uncertainty assessment has been carried out using covariance data recently processed by joint efforts of several US and European Labs. In general, the uncertainty analysis shows that for the wide selection of fast reactor concepts considered, the present integral parameters uncertainties resulting from the assumed uncertainties on nuclear data are probably acceptable in the early phases of design feasibility studies. However, in the successive phase of preliminary conceptual designs and in later design phases of selected reactor and fuel cycle concepts, there will be the need for improved data and methods, in order to reduce margins, both for economic and safety reasons. It is then important to define as soon as possible priority issues, i.e. which are the nuclear data (isotope, reaction type, energy range) that need improvement, in order to quantify target accuracies and to select a strategy to meet the requirements needed (e.g. by some selected new differential measurements and by the use of integral experiments). In this context one should account for the wide range of high accuracy integral experiments already performed and available in national or, better, international data basis, in order to indicate new integral experiments that will be needed to account for new requirements due to innovative design features, and to provide the necessary full integral data base to be used for validation of the design simulation tools.

  18. Criteria for selection of target materials and design of high-efficiency-release targets for radioactive ion beam generation

    CERN Document Server

    Alton, G D; Liu, Y

    1999-01-01

    In this report, we define criteria for choosing target materials and for designing, mechanically stable, short-diffusion-length, highly permeable targets for generation of high-intensity radioactive ion beams (RIBs) for use at nuclear physics and astrophysics research facilities based on the ISOL principle. In addition, lists of refractory target materials are provided and examples are given of a number of successful targets, based on these criteria, that have been fabricated and tested for use at the Holifield Radioactive Ion Beam Facility (HRIBF).

  19. Radioactive targets for nuclear astrophysics research at LANSCE

    International Nuclear Information System (INIS)

    Koehler, P.E.; O'Brien, H.A.; Gursky, J.C.

    1990-01-01

    During the past few years we have made measurements of (n,p) and (n,α) cross sections on several radioactive nuclei of importance to nuclear astrophysics. The measurements were made at the Manuel Lujan, Jr., Neutron Scattering Center (LANSCE) from thermal neutron energy to approximately 100 keV. Successful measurements have been completed on the radioisotopes 7 Be, 22 Na and 36 Cl while preliminary data have been taken on targets of 54 Mn and 55 Fe. Similar measurements have also been made on the stable isotopes 14 N, 17 O and 35 Cl. We are currently assembling a 4π barium fluoride (BaF 2 ) detector which will allow us to expand our program to (n,γ) measurements. The (n,γ) (and in some cases future (n,p)) measurements will require targets with higher specific activity and greater chemical purity than we have so far been able to use. We discuss the fabrication techniques used for the samples produced so far, the requirements the future (n,γ) targets must meet and our current plans for producing them, and the physics motivations for the measurements

  20. Nuclear safety targets and problems of social acceptability

    International Nuclear Information System (INIS)

    Macgill, S.M.

    1989-01-01

    The following are among the factors which make the problem of setting acceptable safety targets for societal protection from possible nuclear accidents one of such formidable proportion: The varied and often conflicting positions among and between the many constituencies with a claim to interest in the problem: local, national and international populations; lay, workplace and professional communities; private and public interests; active environmental lobbies and intentionally passive publics; powerful influences and politically unprivileged classes; press and mass media. To seek 'acceptability' of safety targets through common consensus is problematised by the difficulty in overcoming the immense social and historical forces that give rise to the prevailing contrariety among different people's positions. To seek resolution of differences by some appropriate weighting of the different views of different constituencies is problematised by the lack of unique identification of what the constituencies are, by the difficulty in faithfully representing their views, and by the absence of 'laws of social entitlement' vis-a-vis the weight that should be given to each. In sum, the problem of setting socially acceptable safety targets is itself bound up with inherently open ended questions of democracy and representation. (author)

  1. An efficient and cost effective nuclear medicine image network

    International Nuclear Information System (INIS)

    Sampathkumaran, K.S.; Miller, T.R.

    1987-01-01

    An image network that is in use in a large nuclear medicine department is described. This network was designed to efficiently handle a large volume of clinical data at reasonable cost. Small, limited function computers are attached to each scintillation camera for data acquisition. The images are transferred by cable network or floppy disc to a large, powerful central computer for processing and display. Cost is minimized by use of small acquisition computers not equipped with expensive video display systems or elaborate analysis software. Thus, financial expenditure can be concentrated in a powerful central computer providing a centralized data base, rapid processing, and an efficient environment for program development. Clinical work is greatly facilitated because the physicians can process and display all studies without leaving the main reading area. (orig.)

  2. The high efficiency steel filters for nuclear air cleaning

    International Nuclear Information System (INIS)

    Bergman, W.; Larsen, G.; Lopez, R.; Williams, K.; Violet, C.

    1990-08-01

    We have, in cooperation with industry, developed high-efficiency filters made from sintered stainless-steel fibers for use in several air-cleaning applications in the nuclear industry. These filters were developed to overcome the failure modes in present high-efficiency particulate air (HEPA) filters. HEPA filters are made from glass paper and glue, and they may fail when they get hot or wet and when they are overpressured. In developing our steel filters, we first evaluated the commercially available stainless-steel filter media made from sintered powder and sintered fiber. The sintered-fiber media performed much better than sintered-powder media, and the best media had the smallest fiber diameter. Using the best media, we then built prototype filters for venting compressed gases and evaluated them in our automated filter tester. 12 refs., 20 figs

  3. High efficiency steel filters for nuclear air cleaning

    International Nuclear Information System (INIS)

    Bergman, W.; Conner, J.; Larsen, G.; Lopez, R.; Turner, C.; Vahla, G.; Violet, C.; Williams, K.

    1991-01-01

    The authors have, in cooperation with industry, developed high-efficiency filters made from sintered stainless-steel fibers for use in several air-cleaning applications in the nuclear industry. These filters were developed to overcome the failure modes in present high-efficiently particulate air (HEPA) filters. HEPA filters are made from glass paper and glue, and they may fail when they get hot or wet and when they are overpressured. In developing steel filters, they first evaluated the commercially available stainless-steel filter media made from sintered powder and sintered fiber. The sintered-fiber media performed much better than sintered-powder media, and the best media had the smallest fiber diameter. Using the best media, prototype filters were then built for venting compressed gases and evaluated in their automated filter tester

  4. Thin-thick hydrogen target for nuclear physics

    Energy Technology Data Exchange (ETDEWEB)

    Gheller, J.-M.; Juster, F.-P.; Authelet, G. [CEA Saclay, Irfu/SACM, F-91191 Gif-Sur-Yvette cedex (France); Vinyar, I. [PELIN Limited Liability Company 27 A, Gzhatskaya Str, office 103 St. Petersbourg 195220 (Russian Federation); Relland, J. [CEA Saclay, Irfu/SIS, F-91191 Gif-Sur-Yvette cedex (France); Commeaux, C. [Institut de Physique Nucléaire, campus Universitaire-Bat 103, 91406 Orsay cedex (France)

    2014-01-29

    In spectroscopic studies of unstable nuclei, hydrogen targets are of key importance. The CHyMENE Project aims to provide to the nuclear physics community a thin and pure solid windowless hydrogen or deuterium target. CHyMENE project must respond to this request for the production of solid Hydrogen. The solid hydrogen target is produced in a continuous flow (1 cm/s) by an extrusion technique (developed with the PELIN laboratory) in a vacuum chamber. The shape of the target is determined by the design of the nozzle at the extrusion process. For the purpose, the choice is a rectangular shape with a width of 10 mm and a thickness in the range of 30-50 microns necessary for the physics objectives. The cryostat is equipped with a GM Cryocooler with sufficient power for the solidification of the hydrogen in the lower portion of the extruder. In the higher part of the cryostat, the hydrogen gas is first liquefied and partially solidified. It is then compressed at 100 bars in the cooled extruder before expulsion of the film through the nozzle at the center of the reaction vacuum chamber. After the previous step, the solid hydrogen ribbon falls by gravity into a dedicated chamber where it sublimes and the gas is pumped and evacuated in a exhaust line. This paper deals with the design of the cryostat with its equipment, with the sizing of the thermal bridge (Aluminum and copper), with the results regarding the contact resistance as well as with the vacuum computations of the reaction and recovery hydrogen gas chambers.

  5. Consensus together to jointly promote the safe and efficient development of China's Nuclear industry

    International Nuclear Information System (INIS)

    Lei Zengguang

    2012-01-01

    After the development of China's nuclear industry 56 years, and a certain ability to form a strategic advantage for sustainable development, laying a solid foundation for the development of the national nuclear energy. 2011 Japan's Fukushima Daiichi nuclear accident occurred seven. 2011 of the 'Economic and Social Development Twelfth Five Five Year plan' clearly stated: 'on the basis of ensuring efficient and safe development of nuclear power', the development of China's nuclear industry is facing unprecedented opportunities and challenges, requiring the nuclear industry and nuclear academia work together to jointly promote China's nuclear industry safe and efficient, development

  6. Accountability feedback assessments for improving efficiency of nuclear regulatory institutions

    International Nuclear Information System (INIS)

    Lavarenne, Jean; Shwageraus, Eugene; Weightman, Michael

    2016-01-01

    The Fukushima-Daiichi Accident demonstrated the need of assessing and strengthening institutions involved in nuclear safety, including the accountability of regulators. There are a few problems hindering the path towards a greater understanding of accountability systems, the ensemble of mechanisms holding to account the nuclear regulator on behalf of the public. There is no consensus on what it should deliver and no systematic assessment method exists. This article proposes a method of assessing institutions based on defence in depth concepts and inspired from risk-assessment techniques used for nuclear safety. As a first step in testing the proposal, it presents a simple Monte-Carlo simulation, illustrating some of the workings of the method of assessment and demonstrating the kind of results it will be able to supply. This on-going work will assist policy-makers take better informed decisions about the size, structure and organisation of a nuclear regulator and the cost-effective funding of its accountability system. It will assist in striking a balance between efficiency and resilience of regulatory decision-making processes. It will also promote the involvement of stakeholders and allow them to have a more meaningful impact on regulatory decisions, thereby enhancing the robustness of the regulatory system and potentially trust and confidence. - Highlights: •A general introduction to regulatory accountability is given. •A definition of an effective accountability system is proposed. •A method to assess accountability systems is proposed. •A simplified simulation of a regulatory system demonstrates the method’s capabilities.

  7. On the efficiency of conical targets for laser thermonuclear fusion

    International Nuclear Information System (INIS)

    Borovskij, A.V.; Korobkin, V.V.

    1981-01-01

    Advantages and drawbacks of conical targets (CT) for laser fusion (LF) are discussed. Possibility of the laser power reduction, laser pulse lengthening and neutron yield increase are analyzed for an ideal conical target with absolutely rigid and heat-proof walls as compared to a spherical target of the same mass. A simple theory is suggested which makes it possible to take into account an effect of walls on the fusion process in the conical target with gaseous fuel and heavy shell. Energy losses due to wall deformations and heat conduction are estimated. An influence of these effects on the neutron yield is estimated. CT used in the LF experiments are found to have serious drawbacks in comparison with spherical ones. These drawbacks are connected with the effect of walls on the processes taking place in CT. Analysis of CT, for which the effect of walls is not significant, points up some definite advantages of CT as compared with spherical one. These advantages are the possibility of laser pulse lengthening and laser power reduction in comparison with the irradiation of a sphere of an equal mass. These two positive qualities are connected with the fact that CT has large linear dimensions [ru

  8. Energy efficiency improvement target for SIC 34 - fabricated metal products

    Energy Technology Data Exchange (ETDEWEB)

    Byrer, T. G.; Billhardt, C. F.; Farkas, M. S.

    1977-03-15

    A March 15, 1977 revision of a February 15, 1977 document on the energy improvement target for the Fabricated Metal Products industry (SIC 34) is presented. A net energy savings in 1980 of 24% as compared with 1972 energy consumption in SIC 34 is considered a realistic goal. (ERA citation 04:045008)

  9. Contribution of the residue at position 4 within classical nuclear localization signals to modulating interaction with importins and nuclear targeting.

    Science.gov (United States)

    Smith, Kate M; Di Antonio, Veronica; Bellucci, Luca; Thomas, David R; Caporuscio, Fabiana; Ciccarese, Francesco; Ghassabian, Hanieh; Wagstaff, Kylie M; Forwood, Jade K; Jans, David A; Palù, Giorgio; Alvisi, Gualtiero

    2018-08-01

    Nuclear import involves the recognition by importin (IMP) superfamily members of nuclear localization signals (NLSs) within protein cargoes destined for the nucleus, the best understood being recognition of classical NLSs (cNLSs) by the IMPα/β1 heterodimer. Although the cNLS consensus [K-(K/R)-X-(K/R) for positions P2-P5] is generally accepted, recent studies indicated that the contribution made by different residues at the P4 position can vary. Here, we apply a combination of microscopy, molecular dynamics, crystallography, in vitro binding, and bioinformatics approaches to show that the nature of residues at P4 indeed modulates cNLS function in the context of a prototypical Simian Virus 40 large tumor antigen-derived cNLS (KKRK, P2-5). Indeed, all hydrophobic substitutions in place of R impaired binding to IMPα and nuclear targeting, with the largest effect exerted by a G residue at P4. Substitution of R with neutral hydrophobic residues caused the loss of electrostatic and van der Waals interactions between the P4 residue side chains and IMPα. Detailed bioinformatics analysis confirmed the importance of the P4 residue for cNLS function across the human proteome, with specific residues such as G being associated with low activity. Furthermore, we validate our findings for two additional cNLSs from human cytomegalovirus (HCMV) DNA polymerase catalytic subunit UL54 and processivity factor UL44, where a G residue at P4 results in a 2-3-fold decrease in NLS activity. Our results thus showed that the P4 residue makes a hitherto poorly appreciated contribution to nuclear import efficiency, which is essential to determining the precise nuclear levels of cargoes. Copyright © 2018 Elsevier B.V. All rights reserved.

  10. Innovative-Simplified Nuclear Power Plant Efficiency Evaluation with High-Efficiency Steam Injector System

    International Nuclear Information System (INIS)

    Shoji, Goto; Shuichi, Ohmori; Michitsugu, Mori

    2006-01-01

    It is possible to establish simplified system with reduced space and total equipment weight using high-efficiency Steam Injectors (SI) instead of low-pressure feedwater heaters in Nuclear Power Plant (NPP). The SI works as a heat exchanger through direct contact between feedwater from condensers and extracted steam from turbines. It can get higher pressure than supplied steam pressure. The maintenance and reliability are still higher than the feedwater ones because SI has no movable parts. This paper describes the analysis of the heat balance, plant efficiency and the operation of this Innovative-Simplified NPP with high-efficiency SI. The plant efficiency and operation are compared with the electric power of 1100 MWe-class BWR system and the Innovative-Simplified BWR system with SI. The SI model is adapted into the heat balance simulator with a simplified model. The results show that plant efficiencies of the Innovated-Simplified BWR system are almost equal to original BWR ones. The present research is one of the projects that are carried out by Tokyo Electric Power Company, Toshiba Corporation, and six Universities in Japan, funded from the Institute of Applied Energy (IAE) of Japan as the national public research-funded program. (authors)

  11. Efficiency improvement of nuclear power plant operation: the significant role of advanced nuclear fuel technologies

    International Nuclear Information System (INIS)

    Velde Van de, A.; Burtak, F.

    2001-01-01

    Due to the increased liberalisation of the power markets, nuclear power generation is being exposed to high cost reduction pressure. In this paper we highlight the role of advanced nuclear fuel technologies to reduce the fuel cycle costs and therefore increase the efficiency of nuclear power plant operation. The key factor is a more efficient utilisation of the fuel and present developments at Siemens are consequently directed at (i) further increase of batch average burnup, (ii) improvement of fuel reliability, (iii) enlargement of fuel operation margins and (iv) improvement of methods for fuel design and core analysis. As a result, the nuclear fuel cycle costs for a typical LWR have been reduced during the past decades by about US$ 35 million per year. The estimated impact of further burnup increases on the fuel cycle costs is expected to be an additional saving of US$10 - 15 million per year. Due to the fact that the fuel will operate closer to design limits, a careful approach is required when introducing advanced fuel features in reload quantities. Trust and co-operation between the fuel vendors and the utilities is a prerequisite for the common success. (authors)

  12. Targeted initiatives. Support for nuclear engineering education in the USA

    International Nuclear Information System (INIS)

    Gutteridge, John

    2001-01-01

    Recruitment and education of a new generation of nuclear engineers stands to benefit in the USA from a range of programmes involving governmental bodies, universities, and industry groups. They are part of efforts to attract more students to consider and prepare for careers in the nuclear industry, and to provide financial support for nuclear research and education. Career prospects in the nuclear field are brightening. The demand for nuclear engineers and nuclear trained personnel is on the rise as the new century opens. During the past year several studies were completed in an attempt to ascertain the problems in nuclear engineering education and define initiatives to address these problems

  13. Kinetic analysis of the effects of target structure on siRNA efficiency

    Science.gov (United States)

    Chen, Jiawen; Zhang, Wenbing

    2012-12-01

    RNAi efficiency for target cleavage and protein expression is related to the target structure. Considering the RNA-induced silencing complex (RISC) as a multiple turnover enzyme, we investigated the effect of target mRNA structure on siRNA efficiency with kinetic analysis. The 4-step model was used to study the target cleavage kinetic process: hybridization nucleation at an accessible target site, RISC-mRNA hybrid elongation along with mRNA target structure melting, target cleavage, and enzyme reactivation. At this model, the terms accounting for the target accessibility, stability, and the seed and the nucleation site effects are all included. The results are in good agreement with that of experiments which show different arguments about the structure effects on siRNA efficiency. It shows that the siRNA efficiency is influenced by the integrated factors of target's accessibility, stability, and the seed effects. To study the off-target effects, a simple model of one siRNA binding to two mRNA targets was designed. By using this model, the possibility for diminishing the off-target effects by the concentration of siRNA was discussed.

  14. Characterization of nuclear physics targets using Rutherford backscattering and particle induced X-ray emission

    International Nuclear Information System (INIS)

    Rubehn, T.; Wozniak, G.J.; Phair, L.; Moretto, L.G.; Yu, K.M.

    1997-01-01

    Rutherford backscattering and particle induced X-ray emission have been utilized to precisely characterize targets used in nuclear fission experiments. The method allows for a fast and non-destructive determination of target thickness, homogeneity and element composition. (orig.)

  15. Thermal performance and efficiency of supercritical nuclear reactors

    International Nuclear Information System (INIS)

    Romney Duffey; Tracy Zhou; Hussam Khartabil

    2009-01-01

    The paper reviews the major advances and innovative aspects of the thermal performance of recent concepts for super-critical water-cooled nuclear reactors (SCWR). The concepts are based on the extensive experience in the thermal power industry with super and ultra-supercritical boilers and turbines. The challenges and goals of increased efficiency, reduced cost, enhanced safety and co-generation have been pursued over the last ten years, and have resulted both in viable concepts and a vibrant defined R and D effort. The supercritical concept has wide acceptance among industry, as it reflects standard engineering practices and current thermal plant technology that is being already deployed. The SCWR concept represents a continuous development of water-cooled reactor technology, which utilizes the best and latest advances made in the thermal power industry. (author)

  16. Towards an efficient multiphysics model for nuclear reactor dynamics

    Directory of Open Access Journals (Sweden)

    Obaidurrahman K.

    2015-01-01

    Full Text Available Availability of fast computer resources nowadays has facilitated more in-depth modeling of complex engineering systems which involve strong multiphysics interactions. This multiphysics modeling is an important necessity in nuclear reactor safety studies where efforts are being made worldwide to combine the knowledge from all associated disciplines at one place to accomplish the most realistic simulation of involved phenomenon. On these lines coupled modeling of nuclear reactor neutron kinetics, fuel heat transfer and coolant transport is a regular practice nowadays for transient analysis of reactor core. However optimization between modeling accuracy and computational economy has always been a challenging task to ensure the adequate degree of reliability in such extensive numerical exercises. Complex reactor core modeling involves estimation of evolving 3-D core thermal state, which in turn demands an expensive multichannel based detailed core thermal hydraulics model. A novel approach of power weighted coupling between core neutronics and thermal hydraulics presented in this work aims to reduce the bulk of core thermal calculations in core dynamics modeling to a significant extent without compromising accuracy of computation. Coupled core model has been validated against a series of international benchmarks. Accuracy and computational efficiency of the proposed multiphysics model has been demonstrated by analyzing a reactivity initiated transient.

  17. A description of a wide beam saddle field ion source used for nuclear target applications

    International Nuclear Information System (INIS)

    Greene, J.P.; Schiel, S.L.; Thomas, G.E.

    1997-01-01

    A description is given of a new, wide beam saddle field sputter source used for the preparation of targets applied in nuclear physics experiments. The ion source characteristics are presented and compared with published results obtained with other sources. Deposition rates acquired utilizing this source are given for a variety of target materials encountered in nuclear target production. New applications involving target thinning and ion milling are discussed

  18. Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting

    Directory of Open Access Journals (Sweden)

    Livia S. Carvalho

    2017-09-01

    Full Text Available Retinal gene therapy has come a long way in the last few decades and the development and improvement of new gene delivery technologies has been exponential. The recent promising results from the first clinical trials for inherited retinal degeneration due to mutations in RPE65 have provided a major breakthrough in the field and have helped cement the use of recombinant adeno-associated viruses (AAV as the major tool for retinal gene supplementation. One of the key problems of AAV however, is its limited capacity for packaging genomic information to a maximum of around 4.8 kb. Previous studies have demonstrated that homologous recombination and/or inverted terminal repeat (ITR mediated concatemerization of two overlapping AAV vectors can partially overcome the size limitation and help deliver larger transgenes. The aim of this study was to investigate and compare the use of different AAV dual-vector approaches in the mouse retina using a systematic approach comparing efficiencies in vitro and in vivo using a unique oversized reporter construct. We show that the hybrid approach relying on vector genome concatemerization by highly recombinogenic sequences and ITRs sequence overlap offers the best levels of reconstitution both in vitro and in vivo compared to trans-splicing and overlap strategies. Our data also demonstrate that dose and vector serotype do not affect reconstitution efficiency but a discrepancy between mRNA and protein expression data suggests a bottleneck affecting translation.

  19. Nuclear turbine efficiency improvement by wet steam study

    International Nuclear Information System (INIS)

    Nishikawa, Tsuyoshi; Morson, A.; Markytan, R.

    2000-01-01

    Most of the turbine used at the nuclear power plant are operated at environment of wet steam, which composes of a big factor of its inner loss in comparison with those of the thermal power plant. If an analytical method predictable on behavior of the wet steam is established, it could be upgraded efficiency of the turbine and also reliability against corrosion formed by moisture. This study, therefore, aims at understanding of physical property of the wet steam flow scarcely known at present, development of an optimum turbine cascade design tool reflected by the property, development of a turbine cascade design reducible of steam loss due to wet steam by using the tool, and development on a method of removing moisture in the turbine to its outer portion. For the tool, a new three dimensional flow numerical analysis is necessary to be developed, to aim at accurately and numerically understanding of the behavior of wet steam. As this study is in advancing now, by using a turbine cascade optimized on the wet steam flow and a developed moisture removing apparatus, about 0.6 % of upgrading in turbine efficiency can be predicted in comparison with that of the advanced aero-cascade of the GE Corporation. (G.K.)

  20. High efficiency targets for high gain inertial confinement fusion

    International Nuclear Information System (INIS)

    Gardner, J.H.; Bodner, S.E.

    1986-01-01

    Rocket efficiencies as high as 15% are possible using short wavelength lasers and moderately high aspect ratio pellet designs. These designs are made possible by two recent breakthroughs in physics constraints. First is the development of the Induced Spatial Incoherence (ISI) technique which allows uniform illumination of the pellet and relaxes the constraint of thermal smoothing, permitting the use of short wavelength laser light. Second is the discovery that the Rayleigh-Taylor growth rate is considerably reduced at the short laser wavelengths. By taking advantage of the reduced constraints imposed by nonuniform laser illumination and Rayleigh-Taylor instability, pellets using 1/4 micron laser light and initial aspect ratios of about 10 (with in flight aspect ratios of about 150 to 200) may produce energy gains as high as 200 to 250

  1. Economical preparation of extremely homogeneous nuclear accelerator targets

    International Nuclear Information System (INIS)

    Maier, H.J.

    1983-01-01

    Techniques for target preparation with a minimum consumption of isotopic material are described. The rotating substrate method, which generates extremely homogeneous targets, is discussed in some detail

  2. Optimum nuclear design of target fuel rod for Mo-99 production in HANARO

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Myung Hyun [Kyung Hee University, Seoul (Korea)

    1998-04-01

    Nuclear target design for Mo-99 production in HANARO was performed, KAERI proposed target design was analyzed and its feasibility was shown. Three commercial target designs of Cintichem, ANL and KAERI were tested for the HANARO irradiation an d they all satisfied with design specification. A parametric study was done for target design options and Mo-99 yields ratio and surface heat flux were compared. Tested parameters were target fuel thickness, irradiation location, target axial length, packing density of powder fuel, size of target radius, target geometry, fuel enrichment, fuel composition, and cladding material. Optimized target fuel was designed for both LEU and HEU options. (author). 17 refs., 33 figs., 42 tabs.

  3. Artificial Chemical Reporter Targeting Strategy Using Bioorthogonal Click Reaction for Improving Active-Targeting Efficiency of Tumor.

    Science.gov (United States)

    Yoon, Hong Yeol; Shin, Min Lee; Shim, Man Kyu; Lee, Sangmin; Na, Jin Hee; Koo, Heebeom; Lee, Hyukjin; Kim, Jong-Ho; Lee, Kuen Yong; Kim, Kwangmeyung; Kwon, Ick Chan

    2017-05-01

    Biological ligands such as aptamer, antibody, glucose, and peptide have been widely used to bind specific surface molecules or receptors in tumor cells or subcellular structures to improve tumor-targeting efficiency of nanoparticles. However, this active-targeting strategy has limitations for tumor targeting due to inter- and intraheterogeneity of tumors. In this study, we demonstrated an alternative active-targeting strategy using metabolic engineering and bioorthogonal click reaction to improve tumor-targeting efficiency of nanoparticles. We observed that azide-containing chemical reporters were successfully generated onto surface glycans of various tumor cells such as lung cancer (A549), brain cancer (U87), and breast cancer (BT-474, MDA-MB231, MCF-7) via metabolic engineering in vitro. In addition, we compared tumor targeting of artificial azide reporter with bicyclononyne (BCN)-conjugated glycol chitosan nanoparticles (BCN-CNPs) and integrin α v β 3 with cyclic RGD-conjugated CNPs (cRGD-CNPs) in vitro and in vivo. Fluorescence intensity of azide-reporter-targeted BCN-CNPs in tumor tissues was 1.6-fold higher and with a more uniform distribution compared to that of cRGD-CNPs. Moreover, even in the isolated heterogeneous U87 cells, BCN-CNPs could bind artificial azide reporters on tumor cells more uniformly (∼92.9%) compared to cRGD-CNPs. Therefore, the artificial azide-reporter-targeting strategy can be utilized for targeting heterogeneous tumor cells via bioorthogonal click reaction and may provide an alternative method of tumor targeting for further investigation in cancer therapy.

  4. Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases

    Directory of Open Access Journals (Sweden)

    Yu-Guo Yuan

    2017-08-01

    Full Text Available Objective To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF with transcription activator-like effector nucleases. Methods TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT. Results The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23% were confirmed pregnant at 30 d. In second round SCNT, 7 (53%, 4 (31%, and 3 (23% recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion This finding signifies the combined use of TALENs and SCNT can generate bi-allelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

  5. Nordic views on the next generation of EU energy efficiency targets

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-11-15

    EU has a target for energy efficiency for 2020, namely a 20 % reduction in the primary energy consumption relative to the PRIMES 2007 forecast for 2020. According to the new energy efficiency directive, that was agreed upon in June 2012, the target is that primary energy consumption must be no more than 1,474 Mtoe or that final energy consumption must be no more than 1,078 Mtoe by 2020. This is an absolute target for EU as a whole. There are no binding targets for member states. Due to the time lapse from goal setting, to policy formulation and practical implementation it is found that 2020 is just around the corner, and that it is high time to start discussion about the next goals. This could be concrete targets for 2030 and indicative targets for 2050. In this report target design and policy instruments are discussed from a Nordic perspective: Is it relevant to have an energy efficiency target? Should targets for energy efficiency be set in terms of primary energy or final energy? In absolute numbers or in relative numbers? Whether and how to divide the burden sharing? What are the Nordic positions of strength with regard to policy instruments? (LN)

  6. Supplementary Material for: DASPfind: new efficient method to predict drug–target interactions

    KAUST Repository

    Ba Alawi, Wail; Soufan, Othman; Essack, Magbubah; Kalnis, Panos; Bajic, Vladimir B.

    2016-01-01

    Abstract Background Identification of novel drug–target interactions (DTIs) is important for drug discovery. Experimental determination of such DTIs is costly and time consuming, hence it necessitates the development of efficient computational

  7. High-Efficiency Retrofit Lessons for Retail from a SuperTarget: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Langner, R.; Deru, M.; Hirsch, A.; Williams, S.

    2013-02-01

    The National Renewable Energy Laboratory partnered with Target under the Commercial Building Program to design and implement a retrofit of a SuperTarget in Thornton, CO. The result was a retrofit design that predicted 37% energy savings over ASHRAE Standard 90.1-2004, and 29% compared to existing (pre-retrofit) store consumption. The largest savings came from energy efficient lighting, energy efficient cooling systems, improved refrigeration, and better control of plug loads.

  8. Toward high-efficiency and detailed Monte Carlo simulation study of the granular flow spallation target

    Science.gov (United States)

    Cai, Han-Jie; Zhang, Zhi-Lei; Fu, Fen; Li, Jian-Yang; Zhang, Xun-Chao; Zhang, Ya-Ling; Yan, Xue-Song; Lin, Ping; Xv, Jian-Ya; Yang, Lei

    2018-02-01

    The dense granular flow spallation target is a new target concept chosen for the Accelerator-Driven Subcritical (ADS) project in China. For the R&D of this kind of target concept, a dedicated Monte Carlo (MC) program named GMT was developed to perform the simulation study of the beam-target interaction. Owing to the complexities of the target geometry, the computational cost of the MC simulation of particle tracks is highly expensive. Thus, improvement of computational efficiency will be essential for the detailed MC simulation studies of the dense granular target. Here we present the special design of the GMT program and its high efficiency performance. In addition, the speedup potential of the GPU-accelerated spallation models is discussed.

  9. Method for detecting binding efficiencies of synthetic oligonucleotides: Targeting bacteria and insects

    Science.gov (United States)

    Expanding applications of gene-based targeting biotechnology in functional genomics and the treatment of plants, animals, and microbes has synergized the need for new methods to measure binding efficiencies of these products to their genetic targets. The adaptation and innovative use of Cell–Penetra...

  10. sup 1 sup 2 sup 9 I targets for studies of nuclear waste transmutation

    CERN Document Server

    Ingelbrecht, C; Raptis, K; Altzitzoglou, T; Noguere, G

    2002-01-01

    Nuclear incineration of long-lived fission products and minor actinides is being investigated as an alternative means of reactor waste disposal. sup 1 sup 2 sup 9 I is of particular interest because of its long half-life and high mobility in the environment. Lead iodide targets of sup 1 sup 2 sup 9 I for neutron capture cross-section measurements were prepared from 210 l fuel reprocessing waste solution containing 1.3 g l sup - sup 1 iodine and other fission products. The iodine was separated by oxidation to I sub 2 and extraction into chloroform, reduction to iodide by sodium sulphite and re-extraction into an aqueous phase. Iodide was precipitated using lead nitrate and dried. The chemistry was carried out batch-wise using 400 ml starting solution each time and recycling the chloroform. An extraction efficiency of about 90%, determined by gamma-ray spectrometry, was achieved.

  11. Learning networks as an enabler for informed decisions to target energy-efficiency potentials in companies

    NARCIS (Netherlands)

    Wohlfarth, Katharina; Eichhammer, W.A.; Schlomann, Barbara; Mielicke, Ursula

    2017-01-01

    his paper deals with possibilities of targeting energy efficiency potentials in German companies by delivering information and support within a cooperative management system “Learning Energy Efficiency Networks” (LEEN). Information deficits are pointed out as a relevant barrier to implementing

  12. Specific and efficient targeting of cyanobacterial bicarbonate transporters to the inner envelope membrane of chloroplasts in Arabidopsis

    Directory of Open Access Journals (Sweden)

    Susumu eUehara

    2016-02-01

    Full Text Available Installation of cyanobacterial bicarbonate transporters to the inner envelope membrane (IEM of chloroplasts in C3 plants has been thought to improve photosynthetic performance. However, the method to deliver cyanobacterial bicarbonate transporters to the chloroplast IEM remains to be established. In this study, we provide evidence that the cyanobacterial bicarbonate transporters, BicA and SbtA, can be specifically installed into the chloroplast IEM using the chloroplast IEM targeting signal in conjunction with the transit peptide. We fused the transit peptide and the mature portion of Cor413im1, whose targeting mechanism to the IEM has been characterized in detail, to either BicA or SbtA isolated from Synechocystis sp. PCC6803. Among the seven chimeric constructs tested, we confirmed that four chimeric bicarbonate transporters, designated as BicAI, BicAII, SbtAII, and SbtAIII, were expressed in Arabidopsis. Furthermore, these chimeric transporters were specifically targeted to the chloroplast IEM. They were also resistant to alkaline extraction but can be solubilized by Triton X-100, indicating that they are integral membrane proteins in the chloroplast IEM. One of the transporters, BicA, could reside in the chloroplast IEM even after removal of the IEM targeting signal. Taken together, our results indicate that the addition of IEM targeting signal, as well as the transit peptide, to bicarbonate transporters allows us to efficiently target nuclear-encoded chimeric bicarbonate transporters to the chloroplast IEM.

  13. Progress in target materials for high-efficiency X-ray backlight

    International Nuclear Information System (INIS)

    Du Ai; Zhou Bin; Li Longxiang; Zhu Xiurong; Li Yu'nong; Shen Jun; Gao Guohua; Zhang Zhihua; Wu Guangming

    2012-01-01

    The composition, microstructure and density of the target materials are the key parameters to determinate the photon energy and intensity of the laser-induced X-ray backlight. Thus the classification of backlight targets, the preparation of target materials and the interaction between targets and high power laser were introduced in this paper. Underdense targets were more competitive than traditional dense targets among the backlight targets. Nano-structured foam targets, which could be classified into nanofiber targets and aerogel targets, were regarded as novel high-efficiency underdense targets. Nanofiber, which was commonly prepared via electro spinning and thermal treatment, exhibited good formability and high concentration of emission atoms; while aerogel, which was prepared via sol-gel processes and supercritical fluid drying, possesses the advantages of homogeneous microstructure and theoretically high conversion efficiency, but accompanied with the disadvantages of complex synthetic processes and low concentration of emission atoms. To prepare monolithic aerogels with low density and high concentration of emission atoms via combined sol-gel theories may be the better design for the development of the laser-induced X-ray backlight. (authors)

  14. Changes to Regulatory Systems for more Efficient Nuclear Energy Deployment: An Industry Viewpoint

    International Nuclear Information System (INIS)

    Pelin, H.

    2016-01-01

    Nuclear energy is required to play a much larger role in the energy mix in most credible energy scenarios that address climate change (680 GW additional capacity by 2050 according to IEA, 1000 GW according to World Nuclear Association). To reach these ambitious targets, a concerted effort will be required involving industry, governments and regulators. Changes to regulatory systems and processes – including licensing (design, site, operation), export control, security and waste - is one important area that can stimulate faster and more cost effective development of nuclear capacity. In the past, regulators were mainly concerned with authorizing a limited number of reactors from a limited number of designs under a national standard. Today regulators need resources to assess a wider range of designs, while each licensee needs to complete a thorough safety assessment even if the design has been assessed and approved elsewhere. These developments are the inevitable consequence of globalization and competition within the industry. This paper examines the current state of nuclear regulation in relation to the main attributes of good regulation as defined by the OECD. It further looks at ongoing efforts among regulators to share experience or harmonize requirements, such as within MDEP, or to agree common safety levels, such as in WENRA, in order to reach common positions and improve their regulatory approaches. Finally, it will assess the work of industry to demonstrate the benefits – both in terms of efficiency as well as safety – of harmonised regulations notably through the activities of the World Nuclear Association/CORDEL Working Group. (author)

  15. Improved laser-to-proton conversion efficiency in isolated reduced mass targets

    Energy Technology Data Exchange (ETDEWEB)

    Morace, A. [Center for Energy Research, University of California, 9500 Gilman Drive, La Jolla, California 92093 (United States); Dipartimento di Fisica, Università degli Studi di Milano, via Celoria 16, 20133 Milano (Italy); Bellei, C.; Patel, P. K. [Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550 (United States); Bartal, T.; Kim, J.; Beg, F. N. [Center for Energy Research, University of California, 9500 Gilman Drive, La Jolla, California 92093 (United States); Willingale, L.; Maksimchuk, A.; Krushelnick, K. [University of Michigan, 2200 Bonisteel Blvd. Ann Arbor, Michigan 48109 (United States); Wei, M. S. [Center for Energy Research, University of California, 9500 Gilman Drive, La Jolla, California 92093 (United States); General Atomics, 3550 General Atomics Court, San Diego, California 92121 (United States); Batani, D. [Univ. Bordeaux, CNRS, CEA, CELIA (Centre Lasers Intenses et Applications), UMR 5107, F-33405 Talence (France); Piovella, N. [Dipartimento di Fisica, Università degli Studi di Milano, via Celoria 16, 20133 Milano (Italy); Stephens, R. B. [General Atomics, 3550 General Atomics Court, San Diego, California 92121 (United States)

    2013-07-29

    We present experimental results of laser-to-proton conversion efficiency as a function of lateral confinement of the refluxing electrons. Experiments were carried out using the T-Cubed laser at the Center for Ultrafast Optical Science, University of Michigan. We demonstrate that the laser-to-proton conversion efficiency increases by 50% with increased confinement of the target from surroundings with respect to a flat target of the same thickness. Three-dimensional hybrid particle-in-cell simulations using LSP code agree with the experimental data. The adopted target design is suitable for high repetition rate operation as well as for Inertial Confinement Fusion applications.

  16. Development of an Efficient Meso- scale Multi-phase Flow Solver in Nuclear Applications

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Taehun [City Univ. (CUNY), NY (United States)

    2015-10-20

    The proposed research aims at formulating a predictive high-order Lattice Boltzmann Equation for multi-phase flows relevant to nuclear energy related application - namely, saturated and sub-cooled boiling in reactors, and liquid- liquid mixing and extraction for fuel cycle separation. An efficient flow solver will be developed based on the Finite Element based Lattice Boltzmann Method (FE- LBM), accounting for phase-change heat transfer and capable of treating multiple phases over length scales from the submicron to the meter. A thermal LBM will be developed in order to handle adjustable Prandtl number, arbitrary specific heat ratio, a wide range of temperature variations, better numerical stability during liquid-vapor phase change, and full thermo-hydrodynamic consistency. Two-phase FE-LBM will be extended to liquid–liquid–gas multi-phase flows for application to high-fidelity simulations building up from the meso-scale up to the equipment sub-component scale. While several relevant applications exist, the initial applications for demonstration of the efficient methods to be developed as part of this project include numerical investigations of Critical Heat Flux (CHF) phenomena in nuclear reactor fuel bundles, and liquid-liquid mixing and interfacial area generation for liquid-liquid separations. In addition, targeted experiments will be conducted for validation of this advanced multi-phase model.

  17. Technology Efficiency Study on Nuclear Power and Coal Power in Guangdong Province Based on DEA

    International Nuclear Information System (INIS)

    Yinong Li; Dong Wang

    2006-01-01

    Guangdong Province has taken the lead in embarking on nuclear power development to resolve its dire lack of primary resources. With the deepening of the on-going structural reform in the electric power sector in China, the market competition scheme is putting electricity generation enterprises under severe strain. Consequently, it is incumbent upon the nuclear power producers to steadily upgrade management, enhance technical capabilities, reduce cost and improve efficiency. At present, gradual application of such efficiency evaluation methodology has already commenced in some sectors in China including the electric power industry. The purpose of this paper is to use the Data Envelopment Analysis (DEA), which is a cutting-edge approach in the efficiency evaluation field - to study the technological efficiency between nuclear power and coal power in Guangdong Province. The DEA results demonstrate that, as far as Guangdong Province is concerned, the technological efficiency of nuclear power is higher than that of coal power in terms of Technological Efficiency (TE), Pure Technology Efficiency (PTE) and Scale Efficiency (SE). The reason is that nuclear power technology is advanced with a much higher equipment availability factor. Under the same scale, the generation output of nuclear power is far higher than that of equivalent coal power generation. With the environmental protection and sustainable development requirements taken into full account, nuclear power constitutes a clean, safe and highly-efficient energy form which should be extensively harnessed in Guangdong Province to fuel its future continuing economic growth. (authors)

  18. High Efficiency of Mixed Th-U Fuel Utilisation in Innovative Nuclear Burning Wave Reactor

    International Nuclear Information System (INIS)

    Fomin, Sergii; Fomin, A.; Mel’nik, Yu.; Pilipenko, V.; Shul’ga, N.

    2013-01-01

    The presentation provides information about nuclear fuel reproduction and the U-Pu fuel cycle; the history of the Breed and Burn concept and the traveling wave concept; the non-stationary theory of nuclear burning wave; the Nuclear Burning Wave in Fast Reactor with U-Pu Fuel; nuclear burning wave in 5m length cylindrical FR for different reactor radius R and about the Reactor Power Control by Reflector Efficiency

  19. Cognitive Targeting: A Coercive Air Power Theory for Conventional Escalation Control Against Nuclear Armed Adversaries

    Science.gov (United States)

    2016-06-01

    strategic,” in the cognitive targeting paradigm , are those that directly disable - in the strategic audience’s mind – the attractiveness or...This study analyses the applicability of three operational targeting paradigms to coerce a nuclear-armed adversary in a regional crisis, while...principles and elements of war and understand the coercive ability of utility targeting (a capabilities-based targeting paradigm , CBTP), axiological

  20. International Nuclear Target Development Society workshop 1983: proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, G. (ed.)

    1983-01-01

    Separate abstracts were prepared for 11 of the 19 papers presented. Eight papers were previously included in the data base. Discussion group session papers on carbon stripper foils, problems in producing heavy-ion targets, and problems in producing general type targets are included. (WHK)

  1. International Nuclear Target Development Society workshop 1983: proceedings

    International Nuclear Information System (INIS)

    Thomas, G.

    1983-01-01

    Separate abstracts were prepared for 11 of the 19 papers presented. Eight papers were previously included in the data base. Discussion group session papers on carbon stripper foils, problems in producing heavy-ion targets, and problems in producing general type targets are included

  2. Nuclear targeting peptide scaffolds for lipofection of nondividing mammalian cells.

    Science.gov (United States)

    Subramanian, A; Ranganathan, P; Diamond, S L

    1999-09-01

    Lipofection of nondividing cells is inefficient because much of the transfected DNA is retained in endosomes, and that which escapes to the cytoplasm enters the nucleus at low rates. To improve the final rate-limiting step of nuclear import, we conjugated a nonclassical nuclear localization signal (NLS) containing the M9 sequence of heterogeneous nuclear ribonucleoprotein (hnRNP) A1, to a cationic peptide scaffold derived from a scrambled sequence of the SV40 T-antigen consensus NLS (ScT). The ScT was added to improve DNA binding of the M9 sequence. Lipofection of confluent endothelium with plasmid complexed with the M9-ScT conjugate resulted in 83% transfection and a 63-fold increase in marker gene expression. The M9-ScT conjugate localized fluorescent plasmid into the nucleus of permeabilized cells, and addition of the nuclear pore blocker wheat germ agglutinin prevented nuclear import. This method of gene transfer may lead to viral- and lipid-free transfection of nondividing cells.

  3. Gene-carried hepatoma targeting complex induced high gene transfection efficiency with low toxicity and significant antitumor activity

    Directory of Open Access Journals (Sweden)

    Zhao QQ

    2012-06-01

    Full Text Available Qing-Qing Zhao,1,2 Yu-Lan Hu,1 Yang Zhou,3 Ni Li,1 Min Han,1 Gu-Ping Tang,4 Feng Qiu,2 Yasuhiko Tabata,5 Jian-Qing Gao,11Institute of Pharmaceutics, Zhejiang University, Hangzhou, China; 2Department of Pharmacy, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; 3Institute of Biochemistry, Iowa State University, Ames, IA, USA; 4Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou, China; 5Institute for Frontier Medical Sciences, Kyoto University, Kyoto, JapanBackground: The success of gene transfection is largely dependent on the development of a vehicle or vector that can efficiently deliver a gene to cells with minimal toxicity.Methods: A liver cancer-targeted specific peptide (FQHPSF sequence was successfully synthesized and linked with chitosan-linked polyethylenimine (CP to form a new targeted gene delivery vector called CPT (CP/peptide. The structure of CPT was confirmed by 1H nuclear magnetic resonance spectroscopy and ultraviolet spectrophotometry. The particle size of CPT/DNA complexes was measured using laser diffraction spectrometry and the cytotoxicity of the copolymer was evaluated by methylthiazol tetrazolium method. The transfection efficiency evaluation of the CP copolymer was performed using luciferase activity assay. Cellular internalization of the CP/DNA complex was observed under confocal laser scanning microscopy. The targeting specificity of the polymer coupled to peptide was measured by competitive inhibition transfection study. The liver targeting specificity of the CPT copolymer in vivo was demonstrated by combining the copolymer with a therapeutic gene, interleukin-12, and assessed by its abilities in suppressing the growth of ascites tumor in mouse model.Results: The results showed that the liver cancer-targeted specific peptide was successfully synthesized and linked with CP to form a new targeted gene delivery vector called CPT. The composition of CPT

  4. Effect of nonlinearity of spin interaction with electromagnetic resonance field on characteristics of polarized nuclear target

    International Nuclear Information System (INIS)

    Vertij, A.A.; Gavrilov, S.P.; Shestopalov, V.P.

    1990-01-01

    Interaction of incident nuclear particle beam with J = 1/2 (neutrons) spin and (J = 1/2) protons with the target substance is considered. It is shown that neutron polarization at the target exit and neutron transparency (G) of the target depend significantly on incident wave amplitude level and physical parameter values which characterize the target, such as target temperature, resonator mirror reflection factor, number of spins interacting with the field, etc. Under interaction of neutrons with a target resonator which features a high mirror reflection factor and low losses for absorption which is not related to magnetic dipole absorption, a bistable response of neutron polarization and G manifests itself. 1 ref

  5. The nuclear, an efficient tool against global warming

    International Nuclear Information System (INIS)

    2009-01-01

    Proposing and commenting some extracts of a book by Francis Sorin (Le nucleaire et la planete), this document aims at showing that nuclear energy production is a tool to struggle against global warming because of its low carbon emission. Some assessments of this characteristic are given and discussed, as well as figures on carbon emissions in different western countries. This document also criticises the statements made by ecologists against nuclear energy. The author put nuclear energy at the same level as energy savings and renewable energies, as means to reach the desirable CO 2 saving level

  6. Accountability Feedback Assessments for Improving Efficiency of Nuclear Regulatory Institutions

    International Nuclear Information System (INIS)

    Lavarenne, J.

    2016-01-01

    The Fukushima-Daiichi Disaster demonstrated the need of assessing and strengthening institutions involved in nuclear safety, including the accountability of nuclear regulators. There are a few problems hindering the path towards a greater understanding of systems of accountability, the ensemble of mechanisms holding to account the nuclear regulator on behalf of the public. There is no consensus on what it should deliver and no systematic method of assessment exists. This poster proposes a definition of an effective accountability system and a method of assessment of institutions based on defence in depth concepts and inspired from risk-assessment techniques used in the nuclear industry. Finally it presents a simple Monte-Carlo simulation that illustrates the inner workings of the method of assessment and shows the kind of results it will be able to supply. (author)

  7. An efficient method of fuel ice formation in moving free-standing ICF/IFE targets

    Science.gov (United States)

    Aleksandrova, I. V.; Bazdenkov, S. V.; Chtcherbakov, V. I.; Gromov, A. I.; Koresheva, E. R.; Koshelev, E. A.; Osipov, I. E.; Yaguzinskiy, L. S.

    2004-04-01

    Currently, research fields related to the elaboration of efficient layering methods for ICF/IFE applications are rapidly expanding. Significant progress has been made in the technology development based on rapid fuel layering inside moving free-standing targets (FST) which is referred to as the FST layering method. This paper presents our new results obtained in this area and describes technologically elegant solutions towards demonstrating a credible pathway for mass production of IFE cryogenic targets.

  8. An efficient method of fuel ice formation in moving free-standing ICF/IFE targets

    International Nuclear Information System (INIS)

    Aleksandrova, I V; Bazdenkov, S V; Chtcherbakov, V I; Gromov, A I; Koresheva, E R; Koshelev, E A; Osipov, I E; Yaguzinskiy, L S

    2004-01-01

    Currently, research fields related to the elaboration of efficient layering methods for ICF/IFE applications are rapidly expanding. Significant progress has been made in the technology development based on rapid fuel layering inside moving free-standing targets (FST) which is referred to as the FST layering method. This paper presents our new results obtained in this area and describes technologically elegant solutions towards demonstrating a credible pathway for mass production of IFE cryogenic targets

  9. Search efficiency of biased migration towards stationary or moving targets in heterogeneously structured environments

    Science.gov (United States)

    Azimzade, Youness; Mashaghi, Alireza

    2017-12-01

    Efficient search acts as a strong selective force in biological systems ranging from cellular populations to predator-prey systems. The search processes commonly involve finding a stationary or mobile target within a heterogeneously structured environment where obstacles limit migration. An open generic question is whether random or directionally biased motions or a combination of both provide an optimal search efficiency and how that depends on the motility and density of targets and obstacles. To address this question, we develop a simple model that involves a random walker searching for its targets in a heterogeneous medium of bond percolation square lattice and used mean first passage time (〈T 〉 ) as an indication of average search time. Our analysis reveals a dual effect of directional bias on the minimum value of 〈T 〉 . For a homogeneous medium, directionality always decreases 〈T 〉 and a pure directional migration (a ballistic motion) serves as the optimized strategy, while for a heterogeneous environment, we find that the optimized strategy involves a combination of directed and random migrations. The relative contribution of these modes is determined by the density of obstacles and motility of targets. Existence of randomness and motility of targets add to the efficiency of search. Our study reveals generic and simple rules that govern search efficiency. Our findings might find application in a number of areas including immunology, cell biology, ecology, and robotics.

  10. Hydrodynamic efficiency and thermal transport in planar target experiments at LLE

    International Nuclear Information System (INIS)

    Boehly, T.; Goldman, L.M.; Seka, W.; Craxton, R.S.

    1984-01-01

    The authors report the results of single beam irradiation of thin CH foils at laser intensities of 10 13 to 10 15 W/cm 2 in 0.8 ns pulses containing 20 to 50 J of 350 nm and 1054 nm light. They also discuss the hydrodynamic efficiency, thermal transport and preheat in these targets. Included is the measurement of the ion blowoff energy distribution and velocity. The efficient acceleration by short wavelength radiation causes target displacements comparable to the spot size resulting in two-dimension effects. The results are adequately modeled with the 2-D hydrocode SAGE using a flux limiter of f=0.04

  11. Enhancing cognate target elution efficiency in gel-free chemical proteomics

    Directory of Open Access Journals (Sweden)

    Branka Radic-Sarikas

    2015-12-01

    Full Text Available Gel-free liquid chromatography mass spectrometry coupled to chemical proteomics is a powerful approach for characterizing cellular target profiles of small molecules. We have previously described a fast and efficient elution protocol; however, altered target profiles were observed. We hypothesised that elution conditions critically impact the effectiveness of disrupting drug-protein interactions. Thus, a number of elution conditions were systematically assessed with the aim of improving the recovery of all classes of proteins whilst maintaining compatibility with immunoblotting procedures. A double elution with formic acid combined with urea emerged as the most efficient and generically applicable elution method for chemical proteomics

  12. Interaction Profiling Identifies the Human Nuclear Exosome Targeting Complex

    DEFF Research Database (Denmark)

    Lubas, Michal Szymon; Christensen, Marianne Skovgaard; Kristiansen, Maiken Søndergaard

    2011-01-01

    from nucleoli, and consistently NEXT is specifically required for the exosomal degradation of promoter upstream transcripts (PROMPTs). We also detect putative homolog TRAMP subunits hTRF4-2 (Trf4p) and ZCCHC7 (Air2p) in hRRP6 and hMTR4 precipitates. However, at least ZCCHC7 function is restricted...... to nucleoli. Our results suggest that human nuclear exosome degradation pathways comprise modules of spatially organized cofactors that diverge from the yeast model....

  13. Note: Fast neutron efficiency in CR-39 nuclear track detectors

    Energy Technology Data Exchange (ETDEWEB)

    Cavallaro, S. [Dipartimento di Fisica ed Astronomia,Università di Catania, Via S. Sofia 44, 95123 Catania, Italy and INFN-LNS, Via S. Sofia 42, 95123 Catania (Italy)

    2015-03-15

    CR-39 samples are commonly employed for fast neutron detection in fusion reactors and in inertial confinement fusion experiments. The literature reported efficiencies are strongly depending on experimental conditions and, in some cases, highly dispersed. The present note analyses the dependence of efficiency as a function of various parameters and experimental conditions in both the radiator-assisted and the stand-alone CR-39 configurations. Comparisons of literature experimental data with Monte Carlo calculations and optimized efficiency values are shown and discussed.

  14. The space distribution of neutrons generated in massive lead target by relativistic nuclear beam

    International Nuclear Information System (INIS)

    Chultem, D.; Damdinsuren, Ts.; Enkh-Gin, L.; Lomova, L.; Perelygin, V.; Tolstov, K.

    1993-01-01

    The present paper is devoted to implementation of solid state nuclear track detectors in the research of the neutron generation in extended lead spallation target. Measured neutrons space distribution inside the lead target and neutron distribution in the thick water moderator are assessed. (Author)

  15. Efficient payload delivery by a bispecific antibody-drug conjugate targeting HER2 and CD63

    DEFF Research Database (Denmark)

    de Goeij, Bart E.C.G.; Vink, Tom; Ten Napel, Hendrik

    2016-01-01

    Antibody-drug conjugates (ADC) are designed to be stable in circulation and to release potent cytotoxic drugs intracellularly following antigen-specific binding, uptake, and degradation in tumor cells. Efficient internalization and routing to lysosomes where proteolysis can take place is therefore......, for the first time, that intracellular trafficking of ADCs can be improved using a bsAb approach that targets the lysosomal membrane protein CD63 and provide a rationale for the development of novel bsADCs that combine tumor-specific targeting with targeting of rapidly internalizing antigens. © 2016 American...

  16. Targeting utility customers to improve energy savings from conservation and efficiency programs

    International Nuclear Information System (INIS)

    Taylor, Nicholas W.; Jones, Pierce H.; Kipp, M. Jennison

    2014-01-01

    Highlights: • Improving DSM program impacts by targeting high energy users. • DSM energy savings potential hinges on pre-participation performance. • Targeting can benefit different utilities and energy efficiency programs. • Overall performance can be improved by up to 250% via targeting strategies. - Abstract: Electric utilities, government agencies, and private interests in the US have committed and continue to invest substantial resources – including billions of dollars of financial capital – in the pursuit of energy efficiency and conservation through demand-side management (DSM) programs. While most of these programs are deemed to be cost effective, and therefore in the public interest, opportunities exist to improve cost effectiveness by targeting programs to those customers with the greatest potential for energy savings. This article details an analysis of three DSM programs offered by three Florida municipal electric utilities to explore such opportunities. First, we estimate programs’ energy savings impacts; second, we measure and compare energy savings across subgroups of program participants as determined by their pre-intervention energy performance, and third, we explore potential changes in program impacts that might be realized by targeting specific customers for participation in the DSM programs. All three programs resulted in statistically significant average (per-participant) energy savings, yet average savings varied widely, with the customers who performed best (i.e., most efficient) before the intervention saving the least energy and those who performed worst (i.e., least efficient) before the intervention saving the most. Assessment of alternative program participation scenarios with varying levels of customer targeting suggests that program impacts could be increased by as much as 80% for a professional energy audit program, just over 100% for a high-efficiency heat pump upgrade program, and nearly 250% for an attic insulation

  17. Operational safety performance and economical efficiency evaluation for nuclear power plants

    International Nuclear Information System (INIS)

    Liu Yachun; Zou Shuliang

    2012-01-01

    The economical efficiency of nuclear power includes a series of environmental parameters, for example, cleanliness. Nuclear security is the precondition and guarantee for its economy, and both are the direct embodiment of the social benefits of nuclear power. Through analyzing the supervision and management system on the effective operation of nuclear power plants, which has been put forward by the International Atomic Energy Agency (IAEA), the World Association of Nuclear Operators (WANO), the U.S. Nuclear Regulatory Commission (NRC), and other organizations, a set of indexs on the safety performance and economical efficiency of nuclear power are explored and established; Based on data envelopment analysis, a DEA approach is employed to evaluate the efficiency of the operation performance of several nuclear power plants, Some primary conclusion are achieved on the basis of analyzing the threshold parameter's sensitivity and relativity which affected operational performance. To address the conflicts between certain security and economical indicators, a multi-objective programming model is established, where top priority is given to nuclear safety, and the investment behavior of nuclear power plant is thereby optimized. (authors)

  18. Increased Energy Efficiency in Slovenian Industry - A Contribution to the Kyoto Target

    International Nuclear Information System (INIS)

    Selan, B.; Urbancic, A.

    1998-01-01

    In Slovenia the actual fast growth of greenhouse emissions will require substantial efforts to fulfil the target set in Kyoto. The end-use emissions in the in the industrial sectors represented one third of the total CO 2 emissions in the country in 1996. The cost-effective potential in the sector for CO 2 emission reduction is significant. In the paper, the most important ongoing energy efficiency activities in the industrial sector are presented: information and awareness building, energy advising to larger industrial consumers, energy audition programme, demonstration programme of energy efficiency technologies, financial incentives for energy efficiency investment and the energy efficiency investment fund. A CO 2 tax has been in force since 1997. The results of an evaluation of energy efficiency strategies in industry in the frame of the project 'Integrated resource planning for the energy efficiency in Slovenia' are discussed from the viewpoint of greenhouse gases reduction targets set by Slovenia, and a brief information on the ongoing and expected post Kyoto activities and studies is given. The most important points of the future GHG reduction strategy related to industrial sector in Slovenia will be focused on intensified energy efficiency programme, increased combined heat and power production (CHP), and the effects of incentives through the CO 2 tax. (author)

  19. Medium effects in DIS from polarized nuclear targets

    Energy Technology Data Exchange (ETDEWEB)

    Fanchiotti, Huner; Garcia Canal, Carlos A.; Tarutina, Tatiana [Universidad Nacional de La Plata, Departamento de Fisica, C.C. 67, La Plata (Argentina); Universidad Nacional de La Plata, IFLP(CONICET), C.C. 67, La Plata (Argentina); Vento, Vicente [Universidad de Valencia, Consejo Superior de Investigaciones Cientificas, Departamento de Fisica Teorica and Instituto de Fisica Corpuscular, Burjassot (Valencia) (Spain)

    2014-07-15

    The behavior of the nucleon structure functions in lepton nuclei deep inelastic scattering, both polarized and unpolarized, due to nuclear structure effects is reanalyzed. The study is performed in two schemes: an x-rescaling approach, and one in which there is an increase of sea quark components in the in-medium nucleon, related to the low-energy N-N interaction. In view of a recent interesting experimental proposal to study the behavior of the proton spin structure functions in nuclei we proceed to compare these approaches in an effort to enlighten the possible phenomenological interest of such difficult experiment. (orig.)

  20. An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites.

    Science.gov (United States)

    Ma, Sanyuan; Liu, Yue; Liu, Yuanyuan; Chang, Jiasong; Zhang, Tong; Wang, Xiaogang; Shi, Run; Lu, Wei; Xia, Xiaojuan; Zhao, Ping; Xia, Qingyou

    2017-04-01

    Genome editing enabled unprecedented new opportunities for targeted genomic engineering of a wide variety of organisms ranging from microbes, plants, animals and even human embryos. The serial establishing and rapid applications of genome editing tools significantly accelerated Bombyx mori (B. mori) research during the past years. However, the only CRISPR system in B. mori was the commonly used SpCas9, which only recognize target sites containing NGG PAM sequence. In the present study, we first improve the efficiency of our previous established SpCas9 system by 3.5 folds. The improved high efficiency was also observed at several loci in both BmNs cells and B. mori embryos. Then to expand the target sites, we showed that two newly discovered CRISPR system, SaCas9 and AsCpf1, could also induce highly efficient site-specific genome editing in BmNs cells, and constructed an integrated CRISPR system. Genome-wide analysis of targetable sites was further conducted and showed that the integrated system cover 69,144,399 sites in B. mori genome, and one site could be found in every 6.5 bp. The efficiency and resolution of this CRISPR platform will probably accelerate both fundamental researches and applicable studies in B. mori, and perhaps other insects. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Technical basis for the proposed high efficiency nuclear fuel program

    International Nuclear Information System (INIS)

    MacDonald, P.E.; Herring, J.S.; Crawford, D.C.; Neimark, L.E.

    1999-01-01

    Greenhouse gas emissions from fossil fired electricity generating stations will dramatically increase over the next 20 years. Nuclear energy is the only fully developed technology able to supply large amounts of electricity without generation of greenhouse gases. However, the problem of noncompetitive economics and public concerns about radioactive waste disposal, safety, and nuclear weapons proliferation may prevent the reemergence of nuclear power as a preferred option for new electric energy generation in the U.S. This paper discusses a new research program to help address these issues, by developing fuel designs capable of burnup values in excess of 60 MWD/kgU. The objectives of the program are to: improve the reliability and robustness of light water reactor fuel, thereby improving safety margins; Significantly increase the energy generated by each fuel loading, thereby achieving longer operating cycles, higher capacity factors, and lower cost electric power; Significantly reduce the volume of spent nuclear fuel discharged for disposal by allowing more energy to be extracted from each fuel element prior to discharge; Develop fuel that is much more proliferation resistant. (author)

  2. Korea's nuclear public information experiences-target groups and communication strategies

    International Nuclear Information System (INIS)

    Chung, J.K.

    1996-01-01

    Why public information activities in Korea are needed is first explained. There are three basic reasons; 1) to secure necessary sites for construction of large nuclear facilities; such as nuclear power plants, radwaste management facilities, and nuclear fuel-cycle related facilities 2) to maintain a friendly relationship between the local communities and the nuclear industries, 3) to promote better understanding about the nation's peaceful nuclear programs to the various target groups. Categorization of target groups and messages are reviewed. By whom the public information programs are implemented is also explained. An orchestrated effort together with the third communicators is stressed. Basic philosophy of nuclear public information programs is introduced. A high-profile information campaign and a low-profile information campaign are explained. Particular information strategies suitable to Korean situation as examined. In addition, the Korean general public perception on nuclear energy is briefly introduced. Also, some real insights of anti-nuclear movement in Korea together with the arguments are reviewed. In conclusion, the paper stresses that nuclear arguments became no more technical matters but almost socio-political issues. (author)

  3. ICF target technology at the Russian Federal Nuclear Center

    International Nuclear Information System (INIS)

    Veselov, A.V.; Drozhin, V.S.; Druzhinin, A.A.; Izgorodin, V.M.; Iiyushechkin, B.N.; Kirillov, G.A.; Komleva, G.V.; Korochkin, A.M.; Medvedev, E.F.; Nikolaev, G.P.; Pikulin, I.V.; Pinegin, A.V.; Punin, V.T.; Romaev, V.N.; Sumatokhin, V.L.; Tarasova, N.N.; Tachaev, G.V.; Cherkesova, I.N.

    1995-01-01

    The main effort of the ICF target fabrication group is support of the experiments performed on the 'ISKRA-4' and 'ISKRA-5' laser systems. The main types of targets used in these experiments are direct drive, inverted corona, and indirect drive. For production of direct drive targets, manufacturing techniques have been developed for both hollow glass and polystyrene microspheres. Hollow glass microspheres are fabricated by free-fall of liquid glass drops or dry gel in a 4 meter vertical kiln. These methods allow us to manufacture glass microspheres with diameters from 50 μm to 1 mm, wall thicknesses from 0.5 to 10 μm, and aspect ratios (radius/ wall) from 20 to 500. The microspheres have a thickness inhomogeneity less than 5% and non-sphericity less than 1%. Polystyrene microspheres are fabricated from polystyrene particles with a blowing agent in a similar vertical kiln. Polystyrene microspheres are fabricated with diameter up to 800 μm and wall thicknesses from 1 to 10 μm. 15 refs., 8 figs

  4. Setting up GHG-based energy efficiency targets in buildings: The Ecolabel

    International Nuclear Information System (INIS)

    José Vinagre Díaz, Juan; Richard Wilby, Mark; Belén Rodríguez González, Ana

    2013-01-01

    The European Union has recently updated the regulations for energy performance of buildings and on the certification of energy-related products. The world is in the process of constructing policy frameworks to underwrite carbon emission reduction targets, best exemplified by the Kyoto Protocol. This requires complex technical and economical concepts to be presented in an understandable, transparent, and justifiable format. A building's energy efficiency was traditionally determined based on its annual consumption relative to some average performance level. Emissions are calculated as a derivative of consumptions and their aggregated values allow verification of the level of fulfillment of the objectives. Here we take a different approach: considering that the greenhouse gas emissions (GHG) objectives must be achieved; hence, we fix the efficiency standard based on emissions objectives, and then derive the corresponding reference values of consumption. Accordingly, we propose a certification scheme for energy efficiency in buildings based on targets of GHG emissions levels. This proposed framework includes both a label, namely the Ecolabel, and a fiche showing a set of indices and complementary information. The Ecolabel is designed to provide a flexible, evolvable, simple to use at the point of application, and transparent framework. - Highlights: • In this paper we consider the interaction between greenhouse gas emission reduction targets and building energy efficiency. • Specifically we propose an ‘‘Ecolabel” for buildings that is a GHG emissions liability index, which forms a labeling process. • The label follows the Kyoto Protocol philosophy and translates national GHG targets to targets for each and every building. • The approach provides both a new form of efficiency rating on which emissions reduction policy can be based

  5. Target-nontarget similarity decreases search efficiency and increases stimulus-driven control in visual search.

    Science.gov (United States)

    Barras, Caroline; Kerzel, Dirk

    2017-10-01

    Some points of criticism against the idea that attentional selection is controlled by bottom-up processing were dispelled by the attentional window account. The attentional window account claims that saliency computations during visual search are only performed for stimuli inside the attentional window. Therefore, a small attentional window may avoid attentional capture by salient distractors because it is likely that the salient distractor is located outside the window. In contrast, a large attentional window increases the chances of attentional capture by a salient distractor. Large and small attentional windows have been associated with efficient (parallel) and inefficient (serial) search, respectively. We compared the effect of a salient color singleton on visual search for a shape singleton during efficient and inefficient search. To vary search efficiency, the nontarget shapes were either similar or dissimilar with respect to the shape singleton. We found that interference from the color singleton was larger with inefficient than efficient search, which contradicts the attentional window account. While inconsistent with the attentional window account, our results are predicted by computational models of visual search. Because of target-nontarget similarity, the target was less salient with inefficient than efficient search. Consequently, the relative saliency of the color distractor was higher with inefficient than with efficient search. Accordingly, stronger attentional capture resulted. Overall, the present results show that bottom-up control by stimulus saliency is stronger when search is difficult, which is inconsistent with the attentional window account.

  6. Efficient and robust identification of cortical targets in concurrent TMS-fMRI experiments

    Science.gov (United States)

    Yau, Jeffrey M.; Hua, Jun; Liao, Diana A.; Desmond, John E.

    2014-01-01

    Transcranial magnetic stimulation (TMS) can be delivered during fMRI scans to evoke BOLD responses in distributed brain networks. While concurrent TMS-fMRI offers a potentially powerful tool for non-invasively investigating functional human neuroanatomy, the technique is currently limited by the lack of methods to rapidly and precisely localize targeted brain regions – a reliable procedure is necessary for validly relating stimulation targets to BOLD activation patterns, especially for cortical targets outside of motor and visual regions. Here we describe a convenient and practical method for visualizing coil position (in the scanner) and identifying the cortical location of TMS targets without requiring any calibration or any particular coil-mounting device. We quantified the precision and reliability of the target position estimates by testing the marker processing procedure on data from 9 scan sessions: Rigorous testing of the localization procedure revealed minimal variability in coil and target position estimates. We validated the marker processing procedure in concurrent TMS-fMRI experiments characterizing motor network connectivity. Together, these results indicate that our efficient method accurately and reliably identifies TMS targets in the MR scanner, which can be useful during scan sessions for optimizing coil placement and also for post-scan outlier identification. Notably, this method can be used generally to identify the position and orientation of MR-compatible hardware placed near the head in the MR scanner. PMID:23507384

  7. Storage and Containment of Nuclear Targets for Pulsed Fission-Fusion Testing

    Data.gov (United States)

    National Aeronautics and Space Administration — The combined fission-fusion fuel target is the heart of an engine concept that can open the solar system to fast and efficient human exploration. This is a unique...

  8. Safety targets and public risk perceptions in the nuclear field - technical treadmill or institutional responses?

    International Nuclear Information System (INIS)

    Wynne, B.

    1989-01-01

    The context of our treatment of risk perceptions and safety targets is the apparently wide gap between expert judgements of 'objective risks' and public perceptions of those risks. In the nuclear field the latter appear to so multiply the objective risks as seen by the experts, as to make safety targets vastly too strict (whether for routine discharges or for large accidents), thus design extravagantly expensive on any 'rational' criteria. In recent years the nuclear industry has come to terms more with the public perceptions problem, and has accepted that it is legitimate to exercise different, more severe and costly safety standards in the nuclear field if that is what society wants, as it appears to do. Whilst retaining the conviction that this is scientifically unwarranted, the industry has therefore reconciled itself somewhat to more stringent technical safety targets. (author)

  9. Evaluating the efficiency of nuclear energy policies: an empirical examination for 26 countries.

    Science.gov (United States)

    Gozgor, Giray; Demir, Ender

    2017-08-01

    The decarbonization of the global economy is an urgent concern. As a potential solution, it can be important to understand the efficiency of nuclear energy policies. For this purpose, the paper analyzes whether there is a unit root in nuclear energy consumption in 26 countries and it uses the unit root tests with two endogenous (unknown) structural breaks. The paper finds that nuclear energy consumption is stationary around a level and the time trend in 25 of 26 countries and nuclear energy consumption contains a unit root only in France. The paper also discusses the potential implications of the findings.

  10. Super-compactor and grouting. Efficient and safe treatment of nuclear waste

    International Nuclear Information System (INIS)

    Li, Hongyou; Starke, Holger; Muetzel, Wolfgang; Winter, Marc

    2014-01-01

    The conditioning and volume reduction of nuclear waste are increasingly important factors throughout the world. Efficient and safe treatment of nuclear waste therefore plays a decisive role. Babcock Noell designed, manufactured and supplied a complete waste treatment facility for conditioning of the solid radioactive waste of a nuclear power plant to China. This facility consists of a Sorting Station, a Super-Compactor, a Grouting Unit with Capping Device and other auxiliary equipment which is described in more detail in the following article. This article gives an overview of the efficient and safe treatment of nuclear waste. Babcock Noell is a subsidiary of the Bilfinger Power Systems and has 40 years of experience in the field of design, engineering, construction, static and dynamic calculations, manufacturing, installation, commissioning, as well as in the service and operation of a wide variety of nuclear components and facilities worldwide.

  11. Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi

    DEFF Research Database (Denmark)

    Frandsen, Rasmus John Normand; Andersson, Jens A.; Kristensen, Matilde Bylov

    2008-01-01

    Background: The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion...... of coding sequences with fluorescent markers such as GFP are essential for this process. Construction of vectors for these experiments depends on the directional cloning of two homologous recombination sequences on each side of a selection marker gene. Results: Here, we present a USER Friendly cloning based...

  12. Nuclear energy in Lithuania: Its role, efficiency and safety issues

    International Nuclear Information System (INIS)

    Miskinis, V.; Galinis, A.; Streimikiene, D.

    2000-01-01

    This paper describes the present status of the Lithuanian economy and the power sector as well as problems related to further operation of the Ignalina Nuclear Power Plant (Ignalina NPP) which plays a crucial role in the Lithuanian energy sector. Recent studies have validated that it is economical to keep the Ignalina NPP in operation as long as it is possible and the necessary licenses can be obtained. However, its safe operation remains a very important issue determining its lifetime. Development of an infrastructure and activities necessary for safe and reliable operation of the plant are also very important. (author)

  13. Arabidopsis chromatin-associated HMGA and HMGB use different nuclear targeting signals and display highly dynamic localization within the nucleus

    DEFF Research Database (Denmark)

    Launholt, Dorte; Merkle, Thomas; Houben, Andreas

    2006-01-01

    In plants, the chromatin-associated high mobility group (HMG) proteins occur in twosubfamilies termedHMGAandHMGB.The HMGAproteins are characterized by the presence of four AT-hookDNAbinding motifs, and theHMGBproteins contain anHMG boxDNAbinding domain. As architectural factors, theHMGproteins ap......In plants, the chromatin-associated high mobility group (HMG) proteins occur in twosubfamilies termedHMGAandHMGB.The HMGAproteins are characterized by the presence of four AT-hookDNAbinding motifs, and theHMGBproteins contain anHMG boxDNAbinding domain. As architectural factors, the...... of interphase nuclei, whereas none of the proteins associate with condensed mitotic chromosomes. HMGA is targeted to the nucleus by a monopartite nuclear localization signal, while efficient nuclear accumulation of HMGB1/5 requires large portions of the basic N-terminal part of the proteins. The acidic C...

  14. High neutronic efficiency, low current targets for accelerator-based BNCT applications

    International Nuclear Information System (INIS)

    Powell, J.R.; Ludewig, H.; Todosow, M.

    1998-01-01

    The neutronic efficiency of target/filters for accelerator-based BNCT applications is measured by the proton current required to achieve a desirable neutron current at the treatment port (10 9 n/cm 2 /s). In this paper the authors describe two possible targeyt/filter concepts wihch minimize the required current. Both concepts are based on the Li-7 (p,n)Be-7 reaction. Targets that operate near the threshold energy generate neutrons that are close tothe desired energy for BNCT treatment. Thus, the filter can be extremely thin (∼ 5 cm iron). However, this approach has an extremely low neutron yield (n/p ∼ 1.0(-6)), thus requiring a high proton current. The proposed solutino is to design a target consisting of multiple extremely thin targets (proton energy loss per target ∼ 10 keV), and re-accelerate the protons between each target. Targets operating at ihgher proton energies (∼ 2.5 MeV) have a much higher yield (n/p ∼ 1.0(-4)). However, at these energies the maximum neutron energy is approximately 800 keV, and thus a neutron filter is required to degrade the average neutron energy to the range of interest for BNCT (10--20 keV). A neutron filter consisting of fluorine compounds and iron has been investigated for this case. Typically a proton current of approximately 5 mA is required to generate the desired neutron current at the treatment port. The efficiency of these filter designs can be further increased by incorporating neutron reflectors that are co-axial with the neutron source. These reflectors are made of materials which have high scattering cross sections in the range 0.1--1.0 MeV

  15. Formation of large target residues in intermediate energy nuclear collisions

    International Nuclear Information System (INIS)

    Loveland, W.; Aleklett, K.; Sihver, L.; Xu, Z.; Seaborg, G.T.

    1987-04-01

    We have used radiochemical techniques to measure the yields, angular distributions and velocity spectra of the large (A/sub frag/ ≥ 2/3 A/sub tgt/) target residues from the fragmentation of 197 Au by intermediate energy 12 C, 20 Ne, 32 S, 40 Ar, 84 Kr, and 139 La projectiles. The fragment moving frame angular distributions are asymmetric for the lighter projectiles (C-Ar). The fragment velocity spectra are Maxwellian for the Kr induced reactions and non-Maxwellian for the reactions induced by the lighter ions. We interpret these results in terms of a change in the dominant fragment production mechanism(s) from one(s) involving a fast non-equilibrium process for the lighter ions to a slow, equilibrium process for Kr. Comparison of the measured yields and angular distributions with calculations made using a Boltzmann transport equation with appropriate modifications for Pauli blocking, etc., show excellent agreement between data and theory. 12 refs., 12 figs

  16. Sticking efficiency of nanoparticles in high-velocity collisions with various target materials

    International Nuclear Information System (INIS)

    Reissaus, Philipp; Waldemarsson, Tomas; Blum, Juergen; Clement, Dominik; Llamas, Isabel; Mutschke, Harald; Giovane, Frank

    2006-01-01

    In order to find reliable collector surfaces for the Mesospheric Aerosol - Genesis, Interaction and Composition (MAGIC) sounding rocket experiment, intended to collect atmospheric nanoparticles, the sticking efficiency of nanoparticles was measured on several targets of different materials. The nanoparticles were generated by a molecular beam apparatus in Jena, Germany, by laser ablation (Al 2 O 3 particles, diameter 5-50 nm) and by laser pyrolysis (carbon particles, diameter 10-20 nm). In a vacuum environment (>10 -5 mbar) the particles condensed from the gas phase, formed a particle beam, and were accelerated to ∼∼1 km/s. The sticking efficiency on the target materials carbon, gold and grease was measured by a microbalance. Results demonstrate moderate to high sticking probabilities. Thus, the capture and retrieval of atmospheric nanoparticles was found to be quantitatively feasible

  17. Efficient solution method for optimal control of nuclear systems

    International Nuclear Information System (INIS)

    Naser, J.A.; Chambre, P.L.

    1981-01-01

    To improve the utilization of existing fuel sources, the use of optimization techniques is becoming more important. A technique for solving systems of coupled ordinary differential equations with initial, boundary, and/or intermediate conditions is given. This method has a number of inherent advantages over existing techniques as well as being efficient in terms of computer time and space requirements. An example of computing the optimal control for a spatially dependent reactor model with and without temperature feedback is given. 10 refs

  18. Beam transmission efficiency between injector and target in the GANIL complex

    International Nuclear Information System (INIS)

    Beck, R.; Bru, B.; Ricaud, C.

    1984-06-01

    In order to achieve a maximum transmission efficiency, efforts have been made in three directions: beam measurements, understanding of the physical phenomenon, tuning method. The characteristics of the beam extracted from the three cyclotrons have been measured. The ensuing optical effects are analysed. The tuning of the transport-lines, depending on the characteristics of the extracted beams and the required beam properties on the target, is described

  19. IMPACT OF ENERGY GROUP STRUCTURE ON NUCLEAR DATA TARGET ACCURACY REQUIREMENTS FOR ADVANCED REACTOR SYSTEMS

    Energy Technology Data Exchange (ETDEWEB)

    G. Palmiotti; M. Salvatores; H. Hiruta

    2011-06-01

    A target accuracy assessment study using both a fine and a broad energy structure has shown that less stringent nuclear data accuracy requirements are needed for the latter energy structure. However, even though a reduction is observed, still the requirements will be very difficult to be met unless integral experiments are also used to reduce nuclear data uncertainties. Target accuracy assessment is the inverse problem of the uncertainty evaluation. To establish priorities and target accuracies on data uncertainty reduction, a formal approach can be adopted by defining target accuracy on design parameters and finding out required accuracy on data in order to meet them. In fact, the unknown uncertainty data requirements can be obtained by solving a minimization problem where the sensitivity coefficients in conjunction with the constraints on the integral parameters provide the needed quantities for finding the solutions.

  20. Development of the efficient emergency preparedness system for the nuclear critical infrastructure

    International Nuclear Information System (INIS)

    Kostadinov, V.; Marn, J.; Petelin, S.

    2007-01-01

    The evaluation of the critical nuclear infrastructure vulnerability to threats like human occurrences, terrorist attacks and natural disasters and the preparation of emergency response plans with the estimation of optimized costs are of the vital importance for the assurance of a safe nuclear facilities operation and the national security. In the past national emergency systems did not include vulnerability assessments of the critical nuclear infrastructure as the important part of the comprehensive preparedness framework. The fundamental aims of the efficient emergency preparedness and response system are to provide a sustained emergency readiness and to prevent an emergency situation and accidents. But when an event happens the mission is to mitigate consequences and to protect the people and environment against the nuclear and radiological damage. The efficient emergency response system, which would be activated in the case of the nuclear and/or radiological emergency and release of the radioactivity to the environment, is an important element of a comprehensive system of the nuclear and radiation safety. In the article the new methodology for the critical nuclear infrastructure vulnerability assessment as a missing part of an efficient emergency preparedness system is presented. It can help the overall national energy sectors to identify and better understand the terrorist threats and vulnerabilities of their critical infrastructure. The presented methodology could also facilitate national agencies to develop and implement a vulnerability awareness and education programs for their critical assets to enhance the security, reliability and safe operation of the whole energy infrastructure. The vulnerability assessment methodology will also assist nuclear power plants to develop, validate, and disseminate the assessment and survey of new efficient countermeasures. The significant benefits of the new vulnerability assessment research are to increase nuclear power

  1. Goat red blood cells as precursor for iron oxide nanocrystal synthesis to develop nuclear targeted lung cancer therapy

    Energy Technology Data Exchange (ETDEWEB)

    Sreevani, Vellingiri; Shanthi, Krishnamurthy; Kannan, Soundarapandian, E-mail: sk_protein@buc.edu.in

    2013-09-01

    Graphical abstract: - Highlights: • Molecular approach of synthesis of Fe{sub 2}O{sub 3}-NC using goat blood as a bio-precursor. • The method is simple, efficient and environment friendly. • Synthesized nanocrystals were characterized by UV–vis spectroscopy, XRD, SEM, TEM, DLS and EDS. • Nanocrystals exhibited potent cytotoxicity against A549 cancer cell. • Nuclear targeting with expression of caspase-3, caspase-7 and Bcl-2 in A549 cancer cells. - Abstract: In this study, we synthesised iron oxide nanocrystals (Fe{sub 2}O{sub 3}-NC) from goat blood (bio-precursor) using red blood cells (RBC) lysis method (a molecular level approach) for the first time. The formation of Fe{sub 2}O{sub 3}-NC was achieved through a single-phase chemical reduction method. The size distribution of Fe{sub 2}O{sub 3}-NC falls between 20–30 nm for pellet and 100–200 nm for lysate and were found to be crystalline. Fe{sub 2}O{sub 3}-NC demonstrated significant cytotoxicity on A549. We report the direct visualization of interactions between Fe{sub 2}O{sub 3}-NC and the cancer cell nucleus. The active transport of Fe{sub 2}O{sub 3}-NC to the nucleus induces major changes to nuclear phenotype via nuclear envelope invaginations. We further examined the root cause for the involvement of Fe{sub 2}O{sub 3}-NC on the expression of caspase-3, caspase-7 and Bcl-2 in A549 cancer cells. This functional proteomic analysis clearly implies that the lung cancer cell proliferation is perfectly targeted by the biosynthesised Fe{sub 2}O{sub 3}-NC which could provide new insight for nuclear-targeted cancer therapy.

  2. The efficiency of ceramic-faced metal targets at high-velocity impact

    Science.gov (United States)

    Tolkachev, V. F.; Konyaev, A. A.; Pakhnutova, N. V.

    2017-11-01

    The paper represents experimental results and engineering evaluation concerning the efficiency of composite materials to be used as an additional protection during the high- velocity interaction of a tungsten rod with a target in the velocity range of 1...5 km/s. The main parameter that characterizes the high-velocity interaction of a projectile with a layered target is the penetration depth. Experimental data, numerical simulation and engineering evaluation by modified models are used to determine the penetration depth. Boron carbide, aluminum oxide, and aluminum nickelide are applied as a front surface of targets. Based on experimental data and numerical simulation, the main characteristics of ceramics are determined, which allows composite materials to be effectively used as additional elements of protection.

  3. Ultramild protein-mediated click chemistry creates efficient oligonucleotide probes for targeting and detecting nucleic acids

    DEFF Research Database (Denmark)

    Nåbo, Lina J.; Madsen, Charlotte S.; Jensen, Knud J.

    2015-01-01

    Functionalized synthetic oligonucleotides are finding growing applications in research, clinical studies, and therapy. However, it is not easy to prepare them in a biocompatible and highly efficient manner. We report a new strategy to synthesize oligonucleotides with promising nucleic acid...... targeting and detection properties. We focus in particular on the pH sensitivity of these new probes and their high target specificity. For the first time, human copper(I)-binding chaperon Cox17 was applied to effectively catalyze click labeling of oligonucleotides. This was performed under ultramild...... conditions with fluorophore, peptide, and carbohydrate azide derivatives. In thermal denaturation studies, the modified probes showed specific binding to complementary DNA and RNA targets. Finally, we demonstrated the pH sensitivity of the new rhodamine-based fluorescent probes in vitro and rationalize our...

  4. Targeted Delivery of Toxoplasma gondii Antigens to Dendritic Cells Promote Immunogenicity and Protective Efficiency against Toxoplasmosis

    Directory of Open Access Journals (Sweden)

    Zineb Lakhrif

    2018-02-01

    Full Text Available Toxoplasmosis is a major public health problem and the development of a human vaccine is of high priority. Efficient vaccination against Toxoplasma gondii requires both a mucosal and systemic Th1 immune response. Moreover, dendritic cells play a critical role in orchestrating the innate immune functions and driving specific adaptive immunity to T. gondii. In this study, we explore an original vaccination strategy that combines administration via mucosal and systemic routes of fusion proteins able to target the major T. gondii surface antigen SAG1 to DCs using an antibody fragment single-chain fragment variable (scFv directed against DEC205 endocytic receptor. Our results show that SAG1 targeting to DCs by scFv via intranasal and subcutaneous administration improved protection against chronic T. gondii infection. A marked reduction in brain parasite burden is observed when compared with the intranasal or the subcutaneous route alone. DC targeting improved both local and systemic humoral and cellular immune responses and potentiated more specifically the Th1 response profile by more efficient production of IFN-γ, interleukin-2, IgG2a, and nasal IgA. This study provides evidence of the potential of DC targeting for the development of new vaccines against a range of Apicomplexa parasites.

  5. Productivity, utilization efficiency and sward targets for mixed pastures of marandugrass, forage peanut and tropical kudzu

    Directory of Open Access Journals (Sweden)

    Carlos Mauricio Soares de Andrade

    2012-03-01

    Full Text Available This study was carried out to evaluate the productivity and utilization efficiency of a mixed marandugrass (Brachiaria brizantha cv. Marandu, forage peanut (Arachis pintoi cv. Mandobi and tropical kudzu (Pueraria phaseoloides pasture, rotationally stocked at four daily forage allowance levels (6.6, 10.3, 14.3 and 17.9% of live weight, in order to define sward management targets for these mixtures. In each stocking cycle, dry matter (DM accumulation rates, defoliation intensity (%, grazing depth (% and grazed horizon (cm were evaluated. Sward targets were defined according to the sward condition that best conciliated the grass-legume balance and the equilibrium between forage production and utilization. Pastures submitted to higher forage allowance levels showed higher productivity, but were less efficiently utilized. It was not possible to establish sward management targets for marandugrass-tropical kudzu pastures. For marandugrass-forage peanut pastures the best sward state was set with forage allowance of 10.3% of live weight. Under rotational stocking, the following sward targets were suggested for these pastures in the Western Amazon: pre-grazing height of 30-35 cm (June to September or 45-50 cm (October to May and post-grazing sward height of 20-25 cm (June to September or 25-30 cm (October to May.

  6. Direct Cytoplasmic Delivery and Nuclear Targeting Delivery of HPMA-MT Conjugates in a Microtubules Dependent Fashion.

    Science.gov (United States)

    Zhong, Jiaju; Zhu, Xi; Luo, Kui; Li, Lian; Tang, Manlin; Liu, Yanxi; Zhou, Zhou; Huang, Yuan

    2016-09-06

    As the hearts of tumor cells, the nucleus is the ultimate target of many chemotherapeutic agents and genes. However, nuclear drug delivery is always hampered by multiple intracellular obstacles, such as low efficiency of lysosome escape and insufficient nuclear trafficking. Herein, an N-(2-hydroxypropyl) methacrylamide (HPMA) polymer-based drug delivery system was designed, which could achieve direct cytoplasmic delivery by a nonendocytic pathway and transport into the nucleus in a microtubules dependent fashion. A special targeting peptide (MT), derived from an endogenic parathyroid hormone-related protein, was conjugated to the polymer backbone, which could accumulate into the nucleus a by microtubule-mediated pathway. The in vitro studies found that low temperature and NaN3 could not influence the cell internalization of the conjugates. Besides, no obvious overlay of the conjugates with lysosome demonstrated that the polymer conjugates could enter the tumor cell cytoplasm by a nonendocytic pathway, thus avoiding the drug degradation in the lysosome. Furthermore, after suppression of the microtubule dynamics with microtubule stabilizing docetaxel (DTX) and destabilizing nocodazole (Noc), the nuclear accumulation of polymeric conjugates was significantly inhibited. Living cells fluorescence recovery after photobleaching study found that the nuclear import rate of conjugates was 2-fold faster compared with the DTX and Noc treated groups. These results demonstrated that the conjugates transported into the nucleus in a microtubules dependent way. Therefore, in addition to direct cytoplasmic delivery, our peptide conjugated polymeric platform could simultaneously mediate nuclear drug accumulation, which may open a new path for further intracellular genes/peptides delivery.

  7. Efficient immunoglobulin gene disruption and targeted replacement in rabbit using zinc finger nucleases.

    Directory of Open Access Journals (Sweden)

    Tatiana Flisikowska

    Full Text Available Rabbits are widely used in biomedical research, yet techniques for their precise genetic modification are lacking. We demonstrate that zinc finger nucleases (ZFNs introduced into fertilized oocytes can inactivate a chosen gene by mutagenesis and also mediate precise homologous recombination with a DNA gene-targeting vector to achieve the first gene knockout and targeted sequence replacement in rabbits. Two ZFN pairs were designed that target the rabbit immunoglobulin M (IgM locus within exons 1 and 2. ZFN mRNAs were microinjected into pronuclear stage fertilized oocytes. Founder animals carrying distinct mutated IgM alleles were identified and bred to produce offspring. Functional knockout of the immunoglobulin heavy chain locus was confirmed by serum IgM and IgG deficiency and lack of IgM(+ and IgG(+ B lymphocytes. We then tested whether ZFN expression would enable efficient targeted sequence replacement in rabbit oocytes. ZFN mRNA was co-injected with a linear DNA vector designed to replace exon 1 of the IgM locus with ∼1.9 kb of novel sequence. Double strand break induced targeted replacement occurred in up to 17% of embryos and in 18% of fetuses analyzed. Two major goals have been achieved. First, inactivation of the endogenous IgM locus, which is an essential step for the production of therapeutic human polyclonal antibodies in the rabbit. Second, establishing efficient targeted gene manipulation and homologous recombination in a refractory animal species. ZFN mediated genetic engineering in the rabbit and other mammals opens new avenues of experimentation in immunology and many other research fields.

  8. Characterization and development of an active scintillating target for nuclear reaction studies on actinides

    Energy Technology Data Exchange (ETDEWEB)

    Belier, Gilbert, E-mail: gilbert.belier@cea.fr [CEA, DAM, DIF, DPTA, Centre du Grand Rue, 91297 Arpajon (France); Aupiais, Jean; Varignon, Cyril; Vayre, Sylvain [CEA, DAM, DIF, DPTA, Centre du Grand Rue, 91297 Arpajon (France)

    2012-02-01

    This article presents the development of a new kind of active actinide target, based on organic liquid scintillators containing the dissolved isotope. Amongst many advantages one can mention the very high detection efficiency, the Pulse Shape Discrimination capability, the fast response allowing high count rates and good time resolution and the ease of fabrication. The response of this target to fission fragments has been studied. The discrimination of alpha, fission and proton recoil events is demonstrated. The alpha decay and fission detection efficiencies are simulated and compared to measurements. Finally the use of such a target in the context of fast neutron induced reactions is discussed.

  9. Characterization and development of an active scintillating target for nuclear reaction studies on actinides

    International Nuclear Information System (INIS)

    Belier, Gilbert; Aupiais, Jean; Varignon, Cyril; Vayre, Sylvain

    2012-01-01

    This article presents the development of a new kind of active actinide target, based on organic liquid scintillators containing the dissolved isotope. Amongst many advantages one can mention the very high detection efficiency, the Pulse Shape Discrimination capability, the fast response allowing high count rates and good time resolution and the ease of fabrication. The response of this target to fission fragments has been studied. The discrimination of alpha, fission and proton recoil events is demonstrated. The alpha decay and fission detection efficiencies are simulated and compared to measurements. Finally the use of such a target in the context of fast neutron induced reactions is discussed.

  10. BWR Spent Nuclear Fuel Interfacial Bonding Efficiency Study

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Jy-An John [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Jiang, Hao [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-04-30

    The objective of this project is to perform a systematic study of spent nuclear fuel (SNF, also known as “used nuclear fuel” [UNF]) integrity under simulated transportation environments using the Cyclic Integrated Reversible-Bending Fatigue Tester (CIRFT) hot-cell testing technology developed at Oak Ridge National Laboratory (ORNL) in August 2013. Under Nuclear Regulatory Commission (NRC) sponsorship, ORNL completed four benchmark tests, four static tests, and twelve dynamic or cycle tests on H. B. Robinson (HBR) high burn-up (HBU) fuel. The clad of the HBR fuels was made of Zircaloy-4. Testing was continued in fiscal year (FY) 2014 using Department of Energy (DOE) funds. Additional CIRFT testing was conducted on three HBR rods; two specimens failed, and one specimen was tested to over 2.23 × 107 cycles without failing. The data analysis on all the HBR SNF rods demonstrated that it is necessary to characterize the fatigue life of the SNF rods in terms of (1) the curvature amplitude and (2) the maximum absolute of curvature extremes. The maximum extremes are significant because they signify the maximum tensile stress for the outer fiber of the bending rod. CIRFT testing has also addressed a large variation in hydrogen content on the HBR rods. While the load amplitude is the dominant factor that controls the fatigue life of bending rods, the hydrogen content also has an important effect on the lifetime attained at each load range tested. In FY 15, eleven SNF rod segments from the Limerick BWR were tested using the ORNL CIRFT equipment; one test under static conditions and ten tests under dynamic loading conditions. Under static unidirectional loading, a moment of 85 N·m was obtained at a maximum curvature of 4.0 m-1. The specimen did not show any sign of failure during three repeated loading cycles to a similar maximum curvature. Ten cyclic tests were conducted with amplitudes varying from 15.2 to 7.1 N·m. Failure was observed in nine of

  11. Efficiency factor of a chemical nuclear reactor with gamma sources

    International Nuclear Information System (INIS)

    Anguis T, C.

    1975-01-01

    A chemonuclear reactor is simulated in order to calculate the efficiency factor of molecular species in chemical reactions induced by gamma radiation, with the purpose to obtain information for its design and consider the electromagnetic energy as a possible solution to the present problem of energy. The research is based on a mathematical model of succesive Compton processes applied to spherical and cylindrical geometry and corroborated through the absorbed dose and the experimental date of the increase factor, for the radioisotopic sources Co-60 and Cs-137 relating the quantity of energy deposited into various cylinders with the G value, the relation radius/height of the reactor is optimized according to the molecular production. This is illustrated with the radiolysis of a solution of CH 3 OH/H 2 O which forms H 2 and with the obtainment of C 2 H 5 Br that represents and industrial process induced radioactively. The results show a greater energy deposition with Cs-137 but a larger production of H 2 /hr with Co-60, and besides we can find high production values of C 2 H 5 Br. The cylinder with more advantages is that whose relation R/H is of 0.5. It can be concluded that the final selection of the reactor should be made after a more intense study of the used isotope and the source activity. The efficiency factor of H 2 can be increased selecting the appropriate type and concentration of solute of the irradiated aqueous solutions

  12. High quality, high efficiency welding technology for nuclear power plants

    International Nuclear Information System (INIS)

    Aoki, Shigeyuki; Nagura, Yasumi

    1996-01-01

    For nuclear power plants, it is required to ensure the safety under the high reliability and to attain the high rate of operation. In the manufacture and installation of the machinery and equipment, the welding techniques which become the basis exert large influence to them. For the purpose of improving joint performance and excluding human errors, welding heat input and the number of passes have been reduced, the automation of welding has been advanced, and at present, narrow gap arc welding and high energy density welding such as electron beam welding and laser welding have been put to practical use. Also in the welding of pipings, automatic gas metal arc welding is employed. As for the welding of main machinery and equipment, there are the welding of the joints that constitute pressure boundaries, the build-up welding on the internal surfaces of pressure vessels for separating primary water from them, and the sealing welding of heating tubes and tube plates in steam generators. These weldings are explained. The welding of pipings and the state of development and application of new welding methods are reported. (K.I.)

  13. Pyroprocessing oxide spent nuclear fuels for efficient disposal

    International Nuclear Information System (INIS)

    McPheeters, C.C.; Pierce, R.D.; Mulcahey, T.P.

    1994-01-01

    Pyrochemical processing as a means for conditioning spent nuclear fuels for disposal offers significant advantages over the direct disposal option. The advantages include reduction in high-level waste volume; conversion of most of the high-level waste to a low-level waste in which nearly all the transuranics (TRU) have been removed; and incorporation of the TRUs into a stable, highly radioactive waste form suitable for interim storage, ultimate destruction, or repository disposal. The lithium process has been under development at Argonne National Laboratory for use in pyrochemical conditioning of spent fuel for disposal. All of the process steps have been demonstrated in small-scale (0.5-kg simulated spent fuel) experiments. Engineering-scale (20-kg simulated spent fuel) demonstration of the process is underway, and small-scale experiments have been conducted with actual spent fuel from a light water reactor (LWR). The lithium process is simple, operates at relatively low temperatures, and can achieve high decontamination factors for the TRU elements. Ordinary materials, such as carbon steel, can be used for process containment

  14. .A computer program for nuclear lifetimes measurements by DSAM using a self-supporting target

    International Nuclear Information System (INIS)

    Morand, C.; Chan, T.U.

    1981-02-01

    The present Doppler Shift Attenuation Method, for nuclear lifetimes measurements using self supporting target, has already been described. Therefore this paper only mentions the peculiar features of that DSAM, describes several code facilities, comments the subroutines working along the program structure, in order to be easily handled by other physicists

  15. Tests of Micro-Pattern Gaseous Detectors for active target time projection chambers in nuclear physics

    Energy Technology Data Exchange (ETDEWEB)

    Pancin, J., E-mail: pancin@ganil.fr [GANIL, CEA/DSM-CNRS/IN2P3, Bvd H. Becquerel, Caen (France); Damoy, S.; Perez Loureiro, D. [GANIL, CEA/DSM-CNRS/IN2P3, Bvd H. Becquerel, Caen (France); Chambert, V.; Dorangeville, F. [IPNO, CNRS/IN2P3, Orsay (France); Druillole, F. [CEA, DSM/Irfu/SEDI, Gif-Sur-Yvette (France); Grinyer, G.F. [GANIL, CEA/DSM-CNRS/IN2P3, Bvd H. Becquerel, Caen (France); Lermitage, A.; Maroni, A.; Noël, G. [IPNO, CNRS/IN2P3, Orsay (France); Porte, C.; Roger, T. [GANIL, CEA/DSM-CNRS/IN2P3, Bvd H. Becquerel, Caen (France); Rosier, P. [IPNO, CNRS/IN2P3, Orsay (France); Suen, L. [GANIL, CEA/DSM-CNRS/IN2P3, Bvd H. Becquerel, Caen (France)

    2014-01-21

    Active target detection systems, where the gas used as the detection medium is also a target for nuclear reactions, have been used for a wide variety of nuclear physics applications since the eighties. Improvements in Micro-Pattern Gaseous Detectors (MPGDs) and in micro-electronics achieved in the last decade permit the development of a new generation of active targets with higher granularity pad planes that allow spatial and time information to be determined with unprecedented accuracy. A novel active target and time projection chamber (ACTAR TPC), that will be used to study reactions and decays of exotic nuclei at facilities such as SPIRAL2, is presently under development and will be based on MPGD technology. Several MPGDs (Micromegas and Thick GEM) coupled to a 2×2 mm{sup 2} pixelated pad plane have been tested and their performances have been determined with different gases over a wide range of pressures. Of particular interest for nuclear physics experiments are the angular and energy resolutions. The angular resolution has been determined to be better than 1° FWHM for short traces of about 4 cm in length and the energy resolution deduced from the particle range was found to be better than 5% for 5.5 MeV α particles. These performances have been compared to Geant4 simulations. These experimental results validate the use of these detectors for several applications in nuclear physics.

  16. Tests of Micro-Pattern Gaseous Detectors for active target time projection chambers in nuclear physics

    International Nuclear Information System (INIS)

    Pancin, J.; Damoy, S.; Perez Loureiro, D.; Chambert, V.; Dorangeville, F.; Druillole, F.; Grinyer, G.F.; Lermitage, A.; Maroni, A.; Noël, G.; Porte, C.; Roger, T.; Rosier, P.; Suen, L.

    2014-01-01

    Active target detection systems, where the gas used as the detection medium is also a target for nuclear reactions, have been used for a wide variety of nuclear physics applications since the eighties. Improvements in Micro-Pattern Gaseous Detectors (MPGDs) and in micro-electronics achieved in the last decade permit the development of a new generation of active targets with higher granularity pad planes that allow spatial and time information to be determined with unprecedented accuracy. A novel active target and time projection chamber (ACTAR TPC), that will be used to study reactions and decays of exotic nuclei at facilities such as SPIRAL2, is presently under development and will be based on MPGD technology. Several MPGDs (Micromegas and Thick GEM) coupled to a 2×2 mm 2 pixelated pad plane have been tested and their performances have been determined with different gases over a wide range of pressures. Of particular interest for nuclear physics experiments are the angular and energy resolutions. The angular resolution has been determined to be better than 1° FWHM for short traces of about 4 cm in length and the energy resolution deduced from the particle range was found to be better than 5% for 5.5 MeV α particles. These performances have been compared to Geant4 simulations. These experimental results validate the use of these detectors for several applications in nuclear physics

  17. Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice.

    Science.gov (United States)

    Mikami, Masafumi; Toki, Seiichi; Endo, Masaki

    2015-08-01

    The CRISPR/Cas9 system is an efficient tool used for genome editing in a variety of organisms. Despite several recent reports of successful targeted mutagenesis using the CRISPR/Cas9 system in plants, in each case the target gene of interest, the Cas9 expression system and guide-RNA (gRNA) used, and the tissues used for transformation and subsequent mutagenesis differed, hence the reported frequencies of targeted mutagenesis cannot be compared directly. Here, we evaluated mutation frequency in rice using different Cas9 and/or gRNA expression cassettes under standardized experimental conditions. We introduced Cas9 and gRNA expression cassettes separately or sequentially into rice calli, and assessed the frequency of mutagenesis at the same endogenous targeted sequences. Mutation frequencies differed significantly depending on the Cas9 expression cassette used. In addition, a gRNA driven by the OsU6 promoter was superior to one driven by the OsU3 promoter. Using an all-in-one expression vector harboring the best combined Cas9/gRNA expression cassette resulted in a much improved frequency of targeted mutagenesis in rice calli, and bi-allelic mutant plants were produced in the T0 generation. The approach presented here could be adapted to optimize the construction of Cas9/gRNA cassettes for genome editing in a variety of plants.

  18. Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases.

    Science.gov (United States)

    Remy, Séverine; Tesson, Laurent; Menoret, Séverine; Usal, Claire; De Cian, Anne; Thepenier, Virginie; Thinard, Reynald; Baron, Daniel; Charpentier, Marine; Renaud, Jean-Baptiste; Buelow, Roland; Cost, Gregory J; Giovannangeli, Carine; Fraichard, Alexandre; Concordet, Jean-Paul; Anegon, Ignacio

    2014-08-01

    The generation of genetically modified animals is important for both research and commercial purposes. The rat is an important model organism that until recently lacked efficient genetic engineering tools. Sequence-specific nucleases, such as ZFNs, TALE nucleases, and CRISPR/Cas9 have allowed the creation of rat knockout models. Genetic engineering by homology-directed repair (HDR) is utilized to create animals expressing transgenes in a controlled way and to introduce precise genetic modifications. We applied TALE nucleases and donor DNA microinjection into zygotes to generate HDR-modified rats with large new sequences introduced into three different loci with high efficiency (0.62%-5.13% of microinjected zygotes). Two of these loci (Rosa26 and Hprt1) are known to allow robust and reproducible transgene expression and were targeted for integration of a GFP expression cassette driven by the CAG promoter. GFP-expressing embryos and four Rosa26 GFP rat lines analyzed showed strong and widespread GFP expression in most cells of all analyzed tissues. The third targeted locus was Ighm, where we performed successful exon exchange of rat exon 2 for the human one. At all three loci we observed HDR only when using linear and not circular donor DNA. Mild hypothermic (30°C) culture of zygotes after microinjection increased HDR efficiency for some loci. Our study demonstrates that TALE nuclease and donor DNA microinjection into rat zygotes results in efficient and reproducible targeted donor integration by HDR. This allowed creation of genetically modified rats in a work-, cost-, and time-effective manner. © 2014 Remy et al.; Published by Cold Spring Harbor Laboratory Press.

  19. Polarized photoproduction from nuclear targets with arbitrary spin and relation to deep inelastic scattering

    International Nuclear Information System (INIS)

    Hoodbhoy, P.; Massachusetts Inst. of Tech., Cambridge; Quaid-i-Azam Univ., Islamabad

    1990-01-01

    Inclusive photo-production from polarized targets of arbitrary spin is analyzed by using multipoles. The Drell-Hearn-Gerasimov sum rule, which was originally fromulated for spin-1/2 targets, is generalized to all spins and multipoles, and shown to have some interesting consequences. Measurements to test the new rules, or to derive nuclear structure information from them, could be incorporated into existing plans at electron accelerator facilities. Finally, the possible relevance of these generalized sum rules to sum rules measurable in polarized lepton-polarized target deep inelastic inclusive scattering is discussed. (orig.)

  20. Nuclear reactions of medium and heavy target nuclei with high-energy projectiles

    International Nuclear Information System (INIS)

    Kozma, P.; Damdinsuren, C.

    1988-01-01

    The cross sections of a number of target fragmentation products formed in nuclear reactions of 3.65 AGeV 12 C-ions and 3.65 GeV protons with 197 Au have been measured. The measurements have been done by direct counting of irradiated targets with Ge(Li) gamma-spectrometers. Comparison between these and other data has been used to test the hypotheses of factorization and limiting fragmentation. The total cross section for residue production in both reactions indicates that target residues are formed mainly in central collisions

  1. CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency and specificity

    OpenAIRE

    Liang, Chun; Zhu, Houxiang

    2018-01-01

    The CRISPR-Cpf1 system has been successfully applied in genome editing. However, target efficiency of the CRISPR-Cpf1 system varies among different gRNA sequences. We reanalyzed the published CRISPR-Cpf1 gRNAs data and found many sequence and structural features related to their target efficiency. Using machine learning technology, a SVM model was created to predict target efficiency for any given gRNAs. We have developed the first web service application, CRISPR-DT (CRISPR DNA Targeting), to...

  2. Efficient algorithms for flow simulation related to nuclear reactor safety

    International Nuclear Information System (INIS)

    Gornak, Tatiana

    2013-01-01

    Safety analysis is of ultimate importance for operating Nuclear Power Plants (NPP). The overall modeling and simulation of physical and chemical processes occuring in the course of an accident is an interdisciplinary problem and has origins in fluid dynamics, numerical analysis, reactor technology and computer programming. The aim of the study is therefore to create the foundations of a multi-dimensional non-isothermal fluid model for a NPP containment and software tool based on it. The numerical simulations allow to analyze and predict the behavior of NPP systems under different working and accident conditions, and to develop proper action plans for minimizing the risks of accidents, and/or minimizing the consequences of possible accidents. A very large number of scenarios have to be simulated, and at the same time acceptable accuracy for the critical parameters, such as radioactive pollution, temperature, etc., have to be achieved. The existing software tools are either too slow, or not accurate enough. This thesis deals with developing customized algorithm and software tools for simulation of isothermal and non-isothermal flows in a containment pool of NPP. Requirements to such a software are formulated, and proper algorithms are presented. The goal of the work is to achieve a balance between accuracy and speed of calculation, and to develop customized algorithm for this special case. Different discretization and solution approaches are studied and those which correspond best to the formulated goal are selected, adjusted, and when possible, analysed. Fast directional splitting algorithm for Navier-Stokes equations in complicated geometries, in presence of solid and porous obstacles, is in the core of the algorithm. Developing suitable pre-processor and customized domain decomposition algorithms are essential part of the overall algorithm amd software. Results from numerical simulations in test geometries and in real geometries are presented and discussed.

  3. Vulnerability assessment as a missing part of efficient regulatory emergency preparedness system for nuclear critical infrastructure

    International Nuclear Information System (INIS)

    Kostadinov, V.

    2007-01-01

    One introduces a new model to assess the vulnerability of the nuclear infrastructure critical facilities. The new procedure of the vulnerability assessment (the VA) aims to reevaluate the efficiency of the present-day safeguards. On the basis of deeper insight into the VA new strategy and of the elaborated procedure to analyze the hazards for the nuclear power facilities one recommends the key safeguards affecting the damage magnitude [ru

  4. Efficient interruption of infection chains by targeted removal of central holdings in an animal trade network.

    Science.gov (United States)

    Büttner, Kathrin; Krieter, Joachim; Traulsen, Arne; Traulsen, Imke

    2013-01-01

    Centrality parameters in animal trade networks typically have right-skewed distributions, implying that these networks are highly resistant against the random removal of holdings, but vulnerable to the targeted removal of the most central holdings. In the present study, we analysed the structural changes of an animal trade network topology based on the targeted removal of holdings using specific centrality parameters in comparison to the random removal of holdings. Three different time periods were analysed: the three-year network, the yearly and the monthly networks. The aim of this study was to identify appropriate measures for the targeted removal, which lead to a rapid fragmentation of the network. Furthermore, the optimal combination of the removal of three holdings regardless of their centrality was identified. The results showed that centrality parameters based on ingoing trade contacts, e.g. in-degree, ingoing infection chain and ingoing closeness, were not suitable for a rapid fragmentation in all three time periods. More efficient was the removal based on parameters considering the outgoing trade contacts. In all networks, a maximum percentage of 7.0% (on average 5.2%) of the holdings had to be removed to reduce the size of the largest component by more than 75%. The smallest difference from the optimal combination for all three time periods was obtained by the removal based on out-degree with on average 1.4% removed holdings, followed by outgoing infection chain and outgoing closeness. The targeted removal using the betweenness centrality differed the most from the optimal combination in comparison to the other parameters which consider the outgoing trade contacts. Due to the pyramidal structure and the directed nature of the pork supply chain the most efficient interruption of the infection chain for all three time periods was obtained by using the targeted removal based on out-degree.

  5. An aptamer cocktail-functionalized photocatalyst with enhanced antibacterial efficiency towards target bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Song, Min Young [Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792 (Korea, Republic of); Jurng, Jongsoo [Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792 (Korea, Republic of); Department of Energy and Environmental Engineering, University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792 (Korea, Republic of); Park, Young-Kwon [School of Environmental Engineering, University of Seoul, Seoulsiripdae-ro 163, Dongdaemun-gu, Seoul 02504 (Korea, Republic of); Kim, Byoung Chan, E-mail: bchankim@kist.re.kr [Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792 (Korea, Republic of); Department of Energy and Environmental Engineering, University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792 (Korea, Republic of)

    2016-11-15

    Highlights: • Aptamer-conjugated TiO{sub 2} was developed for target-specific bacterial inactivation. • TiO{sub 2}-aptamer cocktail can enhance inactivation of target bacteria faster than TiO{sub 2}. • TiO{sub 2}-aptamer cocktail can enhance inactivation of target bacteria in mixed culture. • Efficient ROS transfer to the bacteria is caused by close contact of TiO{sub 2}-aptamer. - Abstract: We developed TiO{sub 2} particles conjugated with an Escherichia coli surface-specific ssDNA aptamer cocktail (composed of three different aptamers isolated from E. coli) for targeted and enhanced disinfection of E. coli. We examined the target-specific and enhanced inactivation of this composite (TiO{sub 2}-Apc), which were compared to those of TiO{sub 2} conjugated with a single aptamer (one of the three different aptamers, TiO{sub 2}-Aps) and non-modified TiO{sub 2}. We found that TiO{sub 2}-Apc enhanced the inactivation of targeted E. coli under UV irradiation compared to both the non-modified TiO{sub 2} and TiO{sub 2}-Aps. A higher number of TiO{sub 2}-Apc than TiO{sub 2}-Aps particles was observed on the surface of E. coli. The amount of TiO{sub 2}-Apc required to inactivate ∼99.9% of E. coli (10{sup 6} CFU/ml) was 10 times lower than that of non-modified TiO{sub 2}. The close proximity of functionalized particles with E. coli resulting from the interaction between the target surface and the aptamer induced the efficient and fast transfer of reactive oxygen species to the cells. In a mixed culture of different bacteria (E. coli and Staphylococcus epidermidis), TiO{sub 2}-Apc enhanced the inactivation of only E. coli. Taken together, these results support the use of aptamer cocktail-conjugated TiO{sub 2} for improvement of the target-specific inactivation of bacteria.

  6. Advanced cycle efficiency: Generating 40% more power from the nuclear fuel

    Energy Technology Data Exchange (ETDEWEB)

    Duffey, Romney B.; Leung, Laurence

    2010-09-15

    The introduction of supercritical water (SCW) nuclear power plants (NPPs) would improve the overall plant efficiency significantly compared to currently deployed systems. This improvement is attributed to the increase in plant operating conditions. In addition, the implementation of the reheat-channel option into the CANDU SCW NPPs would further enhance the efficiency. Overall, the combination of higher operating conditions and reheat-channel option would lead to overall plant efficiency of about 50% for the CANDU SCW NPPs, compared to 33--35% for currently deployed systems. This represents a whopping 40% improvement in efficiency.

  7. An Energy-Efficient Target Tracking Framework in Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Zhijun Yu

    2009-01-01

    Full Text Available This study devises and evaluates an energy-efficient distributed collaborative signal and information processing framework for acoustic target tracking in wireless sensor networks. The distributed processing algorithm is based on mobile agent computing paradigm and sequential Bayesian estimation. At each time step, the short detection reports of cluster members will be collected by cluster head, and a sensor node with the highest signal-to-noise ratio (SNR is chosen there as reference node for time difference of arrive (TDOA calculation. During the mobile agent migration, the target state belief is transmitted among nodes and updated using the TDOA measurement of these fusion nodes one by one. The computing and processing burden is evenly distributed in the sensor network. To decrease the wireless communications, we propose to represent the belief by parameterized methods such as Gaussian approximation or Gaussian mixture model approximation. Furthermore, we present an attraction force function to handle the mobile agent migration planning problem, which is a combination of the node residual energy, useful information, and communication cost. Simulation examples demonstrate the estimation effectiveness and energy efficiency of the proposed distributed collaborative target tracking framework.

  8. A highly efficient targeted recombination system for engineering linear chromosomes of industrial bacteria Streptomyces.

    Science.gov (United States)

    Pan, Hung-Yin; Chen, Carton W; Huang, Chih-Hung

    2018-04-17

    Soil bacteria Streptomyces are the most important producers of secondary metabolites, including most known antibiotics. These bacteria and their close relatives are unique in possessing linear chromosomes, which typically harbor 20 to 30 biosynthetic gene clusters of tens to hundreds of kb in length. Many Streptomyces chromosomes are accompanied by linear plasmids with sizes ranging from several to several hundred kb. The large linear plasmids also often contain biosynthetic gene clusters. We have developed a targeted recombination procedure for arm exchanges between a linear plasmid and a linear chromosome. A chromosomal segment inserted in an artificially constructed plasmid allows homologous recombination between the two replicons at the homology. Depending on the design, the recombination may result in two recombinant replicons or a single recombinant chromosome with the loss of the recombinant plasmid that lacks a replication origin. The efficiency of such targeted recombination ranges from 9 to 83% depending on the locations of the homology (and thus the size of the chromosomal arm exchanged), essentially eliminating the necessity of selection. The targeted recombination is useful for the efficient engineering of the Streptomyces genome for large-scale deletion, addition, and shuffling.

  9. CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo

    Science.gov (United States)

    Moreno-Mateos, Miguel A.; Vejnar, Charles E.; Beaudoin, Jean-Denis; Fernandez, Juan P.; Mis, Emily K.; Khokha, Mustafa K.; Giraldez, Antonio J.

    2015-01-01

    CRISPR/Cas9 technology provides a powerful system for genome engineering. However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitation. We have analyzed the molecular features that influence sgRNA stability, activity and loading into Cas9 in vivo. We observe that guanine enrichment and adenine depletion increase sgRNA stability and activity, while loading, nucleosome positioning and Cas9 off-target binding are not major determinants. We additionally identified truncated and 5′ mismatch-containing sgRNAs as efficient alternatives to canonical sgRNAs. Based on these results, we created a predictive sgRNA-scoring algorithm (CRISPRscan.org) that effectively captures the sequence features affecting Cas9/sgRNA activity in vivo. Finally, we show that targeting Cas9 to the germ line using a Cas9-nanos-3′-UTR fusion can generate maternal-zygotic mutants, increase viability and reduce somatic mutations. Together, these results provide novel insights into the determinants that influence Cas9 activity and a framework to identify highly efficient sgRNAs for genome targeting in vivo. PMID:26322839

  10. Efficient prevention and compensation of catastrophic risks. The example of damage by nuclear accidents

    International Nuclear Information System (INIS)

    Vanden Borre, T.

    2001-01-01

    This book deals with the liability for damage due to catastrophic risks. The nuclear liability law serves as an example of such a catastrophic risk. The question that we tried to answer is what an efficient compensation scheme for catastrophic risks should look like. This question is dealt with both from a law and an economic point of view and from a comparative point of view. The main element in comparing the laws in different countries is the comparison between Belgian and Dutch civil (nuclear) liability law. But also American nuclear liability law is part of the analysis (the Price-Anderson Act). The book consists of four parts: (nuclear) civil liability law, legal and economic approach, analysis of other compensation systems and conclusions. The big themes in this book are therefore civil (nuclear) liability law, insurance law and environmental liability law [nl

  11. Manufacturing of calcium, lithium and molybdenum targets for use in nuclear physics experiments

    Science.gov (United States)

    Kheswa, N. Y.; Papka, P.; Buthelezi, E. Z.; Lieder, R. M.; Neveling, R.; Newman, R. T.

    2010-02-01

    This paper describes methods used in the manufacturing of chemically reactive targets such as calcium ( natCa), lithium-6 ( 6Li) and molybdenum-97 ( 97Mo) for nuclear physics experiments at the iThemba LABS cyclotron facility (Faure, South Africa). Due to the chemical properties of these materials a suitable and controlled environment was established in order to minimize oxygen contamination of targets. Calcium was prepared by means of vacuum evaporation while lithium was cold rolled to a desired thickness. In the case of molybdenum, the metallic powder was melted under vacuum using an e-gun followed by cold rolling of the metal bead to a desired thickness. In addition, latest developments toward the establishment of a dedicated nuclear physics target laboratory are discussed.

  12. Manufacturing of calcium, lithium and molybdenum targets for use in nuclear physics experiments

    Energy Technology Data Exchange (ETDEWEB)

    Kheswa, N.Y., E-mail: kheswa@tlabs.ac.z [iThemba Laboratory for Accelerator Based Science, P.O. Box 722, Somerset West 7129, Western Cape (South Africa); Papka, P.; Buthelezi, E.Z.; Lieder, R.M.; Neveling, R.; Newman, R.T. [iThemba Laboratory for Accelerator Based Science, P.O. Box 722, Somerset West 7129, Western Cape (South Africa)

    2010-02-11

    This paper describes methods used in the manufacturing of chemically reactive targets such as calcium ({sup nat}Ca), lithium-6 ({sup 6}Li) and molybdenum-97 ({sup 97}Mo) for nuclear physics experiments at the iThemba LABS cyclotron facility (Faure, South Africa). Due to the chemical properties of these materials a suitable and controlled environment was established in order to minimize oxygen contamination of targets. Calcium was prepared by means of vacuum evaporation while lithium was cold rolled to a desired thickness. In the case of molybdenum, the metallic powder was melted under vacuum using an e-gun followed by cold rolling of the metal bead to a desired thickness. In addition, latest developments toward the establishment of a dedicated nuclear physics target laboratory are discussed.

  13. Probabilistic analysis of the efficiency of the damping devices against nuclear fuel container falling

    Science.gov (United States)

    Králik, Juraj

    2017-07-01

    The paper presents the probabilistic and sensitivity analysis of the efficiency of the damping devices cover of nuclear power plant under impact of the container of nuclear fuel of type TK C30 drop. The finite element idealization of nuclear power plant structure is used in space. The steel pipe damper system is proposed for dissipation of the kinetic energy of the container free fall. The experimental results of the shock-damper basic element behavior under impact loads are presented. The Newmark integration method is used for solution of the dynamic equations. The sensitivity and probabilistic analysis of damping devices was realized in the AntHILL and ANSYS software.

  14. Application of digital solutions to help the safe and efficient operation of nuclear power plants

    International Nuclear Information System (INIS)

    Ortega P, F.; Fernandez F, S.

    2017-09-01

    In the search for excellence, the emergence of solutions to digitize nuclear power plants is an opportunity to optimize the operation and safety of them. The new technologies available today in the market, applied under a global vision of the operation, can contribute to the excellent operation of nuclear power plants in terms of efficiency and effectiveness. Tecnatom has a long experience in various areas related to the operation of the plants, giving the aforementioned global vision, essential to develop global solutions that pursue the safe and efficient operation of the operation. (Author)

  15. An Energy-Efficient Sleep Strategy for Target Tracking Sensor Networks

    Directory of Open Access Journals (Sweden)

    Juan FENG

    2014-02-01

    Full Text Available Energy efficiency is very important for sensor networks since sensor nodes have limited energy supply from battery. So far, many researches have been focused on this issue, while less emphasis was placed on the optimal sleep time of each node. This paper proposed an adaptive energy conservation strategy for target tracking based on a grid network structure, where each node autonomously determines when and if to sleep. It allows sensor nodes far away from targets to sleep to save energy and guarantee the tracking accuracy. The proposed approach extend network lifetime by adopting an adaptive sleep scheduling scheme that combines the local power management (PM and the adaptive coordinate PM strategies to schedule the activities of sensor nodes. And each node can choose an optimal sleep time so as to make system adaptive and energy-efficient. We show the performance of our approach in terms of energy drop, comparing it to a naive approach, dynamic PM with fixed sleep time and the coordinate PM strategies. From the experimental results, it is readily seen that the efficiency of the proposed approach.

  16. Nuclear energy and the IAEA: Fostering the efficient and safe use of nuclear power

    International Nuclear Information System (INIS)

    Kinley, D. III

    2006-05-01

    Any discussion of 21st century energy trends must take into account the global energy imbalance. Roughly 1.6 billion people still lack access to modern energy services, and few aspects of development - whether related to living standards, health care or industrial productivity - can take place without the requisite energy supply. As we look to the century before us, the growth in energy demand will be substantial, and 'connecting the unconnected' will be a key to progress. Another challenge will be sustainability; meeting these growing energy needs without creating negative side effects that could compromise the living environment of future generations. Nuclear power is not a 'fix-all', but it will certainly be part of this mix of solutions, and the expectations for the expanding use of nuclear power are rising. In addition to the growth in demand, these expectations are driven by: energy security concerns; nuclear power's low greenhouse gas emissions; and the sustained strong performance of nuclear plants. Each country must make its own energy choices; one size does not fit all. But for those countries interested in making nuclear power part of their sustainable development strategies, the Agency stands ready to offer a broad range of assistance programmes

  17. Nuclear localization signal targeting to macronucleus and micronucleus in binucleated ciliate Tetrahymena thermophila.

    Science.gov (United States)

    Iwamoto, Masaaki; Mori, Chie; Osakada, Hiroko; Koujin, Takako; Hiraoka, Yasushi; Haraguchi, Tokuko

    2018-06-08

    Ciliated protozoa possess two morphologically and functionally distinct nuclei: a macronucleus (MAC) and a micronucleus (MIC). The MAC is transcriptionally active and functions in all cellular events. The MIC is transcriptionally inactive during cell growth, but functions in meiotic events to produce progeny nuclei. Thus, these two nuclei must be distinguished by the nuclear proteins required for their distinct functions during cellular events such as cell proliferation and meiosis. To understand the mechanism of the nuclear transport specific to either MAC or MIC, we identified specific nuclear localization signals (NLSs) in two MAC- and MIC-specific nuclear proteins, macronuclear histone H1 and micronuclear linker histone-like protein (Mlh1), respectively. By expressing GFP-fused fragments of these proteins in Tetrahymena thermophila cells, two distinct regions in macronuclear histone H1 protein were assigned as independent MAC-specific NLSs and two distinct regions in Mlh1 protein were assigned as independent MIC-specific NLSs. These NLSs contain several essential lysine residues responsible for the MAC- and MIC-specific nuclear transport, but neither contains any consensus sequence with known monopartite or bipartite NLSs in other model organisms. Our findings contribute to understanding how specific nuclear targeting is achieved to perform distinct nuclear functions in binucleated ciliates. © 2018 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

  18. Supplementary Material for: DASPfind: new efficient method to predict drug–target interactions

    KAUST Repository

    Ba Alawi, Wail

    2016-01-01

    Abstract Background Identification of novel drug–target interactions (DTIs) is important for drug discovery. Experimental determination of such DTIs is costly and time consuming, hence it necessitates the development of efficient computational methods for the accurate prediction of potential DTIs. To-date, many computational methods have been proposed for this purpose, but they suffer the drawback of a high rate of false positive predictions. Results Here, we developed a novel computational DTI prediction method, DASPfind. DASPfind uses simple paths of particular lengths inferred from a graph that describes DTIs, similarities between drugs, and similarities between the protein targets of drugs. We show that on average, over the four gold standard DTI datasets, DASPfind significantly outperforms other existing methods when the single top-ranked predictions are considered, resulting in 46.17 % of these predictions being correct, and it achieves 49.22 % correct single top ranked predictions when the set of all DTIs for a single drug is tested. Furthermore, we demonstrate that our method is best suited for predicting DTIs in cases of drugs with no known targets or with few known targets. We also show the practical use of DASPfind by generating novel predictions for the Ion Channel dataset and validating them manually. Conclusions DASPfind is a computational method for finding reliable new interactions between drugs and proteins. We show over six different DTI datasets that DASPfind outperforms other state-of-the-art methods when the single top-ranked predictions are considered, or when a drug with no known targets or with few known targets is considered. We illustrate the usefulness and practicality of DASPfind by predicting novel DTIs for the Ion Channel dataset. The validated predictions suggest that DASPfind can be used as an efficient method to identify correct DTIs, thus reducing the cost of necessary experimental verifications in the process of drug discovery

  19. Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress.

    Science.gov (United States)

    LeBlanc, Chantal; Zhang, Fei; Mendez, Josefina; Lozano, Yamile; Chatpar, Krishna; Irish, Vivian F; Jacob, Yannick

    2018-01-01

    The CRISPR/Cas9 system has greatly improved our ability to engineer targeted mutations in eukaryotic genomes. While CRISPR/Cas9 appears to work universally, the efficiency of targeted mutagenesis and the adverse generation of off-target mutations vary greatly between different organisms. In this study, we report that Arabidopsis plants subjected to heat stress at 37°C show much higher frequencies of CRISPR-induced mutations compared to plants grown continuously at the standard temperature (22°C). Using quantitative assays relying on green fluorescent protein (GFP) reporter genes, we found that targeted mutagenesis by CRISPR/Cas9 in Arabidopsis is increased by approximately 5-fold in somatic tissues and up to 100-fold in the germline upon heat treatment. This effect of temperature on the mutation rate is not limited to Arabidopsis, as we observed a similar increase in targeted mutations by CRISPR/Cas9 in Citrus plants exposed to heat stress at 37°C. In vitro assays demonstrate that Cas9 from Streptococcus pyogenes (SpCas9) is more active in creating double-stranded DNA breaks at 37°C than at 22°C, thus indicating a potential contributing mechanism for the in vivo effect of temperature on CRISPR/Cas9. This study reveals the importance of temperature in modulating SpCas9 activity in eukaryotes, and provides a simple method to increase on-target mutagenesis in plants using CRISPR/Cas9. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd.

  20. An Energy-Efficient Target-Tracking Strategy for Mobile Sensor Networks.

    Science.gov (United States)

    Mahboubi, Hamid; Masoudimansour, Walid; Aghdam, Amir G; Sayrafian-Pour, Kamran

    2017-02-01

    In this paper, an energy-efficient strategy is proposed for tracking a moving target in an environment with obstacles, using a network of mobile sensors. Typically, the most dominant sources of energy consumption in a mobile sensor network are sensing, communication, and movement. The proposed algorithm first divides the field into a grid of sufficiently small cells. The grid is then represented by a graph whose edges are properly weighted to reflect the energy consumption of sensors. The proposed technique searches for near-optimal locations for the sensors in different time instants to route information from the target to destination, using a shortest path algorithm. Simulations confirm the efficacy of the proposed algorithm.

  1. Specific and Efficient Regression of Cancers Harboring KRAS Mutation by Targeted RNA Replacement.

    Science.gov (United States)

    Kim, Sung Jin; Kim, Ju Hyun; Yang, Bitna; Jeong, Jin-Sook; Lee, Seong-Wook

    2017-02-01

    Mutations in the KRAS gene, which persistently activate RAS function, are most frequently found in many types of human cancers. Here, we proposed and verified a new approach against cancers harboring the KRAS mutation with high cancer selectivity and efficient anti-cancer effects based on targeted RNA replacement. To this end, trans-splicing ribozymes from Tetrahymena group I intron were developed, which can specifically target and reprogram the mutant KRAS G12V transcript to induce therapeutic gene activity in cells. Adenoviral vectors containing the specific ribozymes with downstream suicide gene were constructed and then infection with the adenoviruses specifically downregulated KRAS G12V expression and killed KRAS G12V-harboring cancer cells additively upon pro-drug treatment, but it did not affect the growth of wild-type KRAS-expressing cells. Minimal liver toxicity was noted when the adenoviruses were administered systemically in vivo. Importantly, intratumoral injection of the adenoviruses with pro-drug treatment specifically and significantly impeded the growth of xenografted tumors harboring KRAS G12V through a trans-splicing reaction with the target RNA. In contrast, xenografted tumors harboring wild-type KRAS were not affected by the adenoviruses. Therefore, RNA replacement with a mutant KRAS-targeting trans-splicing ribozyme is a potentially useful therapeutic strategy to combat tumors harboring KRAS mutation. Copyright © 2017 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.

  2. The roles of energy and material efficiency in meeting steel industry CO2 targets.

    Science.gov (United States)

    Milford, Rachel L; Pauliuk, Stefan; Allwood, Julian M; Müller, Daniel B

    2013-04-02

    Identifying strategies for reducing greenhouse gas emissions from steel production requires a comprehensive model of the sector but previous work has either failed to consider the whole supply chain or considered only a subset of possible abatement options. In this work, a global mass flow analysis is combined with process emissions intensities to allow forecasts of future steel sector emissions under all abatement options. Scenario analysis shows that global capacity for primary steel production is already near to a peak and that if sectoral emissions are to be reduced by 50% by 2050, the last required blast furnace will be built by 2020. Emissions reduction targets cannot be met by energy and emissions efficiency alone, but deploying material efficiency provides sufficient extra abatement potential.

  3. Control of laser absorbing efficiency and proton quality by a specific double target

    Czech Academy of Sciences Publication Activity Database

    Yu, Q.; Gu, Yanjun; Li, X.F.; Qu, J.F.; Kong, Q.; Kawata, S.

    2016-01-01

    Roč. 18, č. 8 (2016), 1-9, č. článku 083024. ISSN 1367-2630 R&D Projects: GA MŠk LQ1606; GA MŠk EF15_008/0000162 Grant - others:ELI Beamlines(XE) CZ.02.1.01/0.0/0.0/15_008/0000162 Institutional support: RVO:68378271 Keywords : improved proton beam quality * increased laser absorption efficiency * specific double-layer target Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 3.786, year: 2016

  4. An Adenovirus DNA Replication Factor, but Not Incoming Genome Complexes, Targets PML Nuclear Bodies.

    Science.gov (United States)

    Komatsu, Tetsuro; Nagata, Kyosuke; Wodrich, Harald

    2016-02-01

    Promyelocytic leukemia protein nuclear bodies (PML-NBs) are subnuclear domains implicated in cellular antiviral responses. Despite the antiviral activity, several nuclear replicating DNA viruses use the domains as deposition sites for the incoming viral genomes and/or as sites for viral DNA replication, suggesting that PML-NBs are functionally relevant during early viral infection to establish productive replication. Although PML-NBs and their components have also been implicated in the adenoviral life cycle, it remains unclear whether incoming adenoviral genome complexes target PML-NBs. Here we show using immunofluorescence and live-cell imaging analyses that incoming adenovirus genome complexes neither localize at nor recruit components of PML-NBs during early phases of infection. We further show that the viral DNA binding protein (DBP), an early expressed viral gene and essential DNA replication factor, independently targets PML-NBs. We show that DBP oligomerization is required to selectively recruit the PML-NB components Sp100 and USP7. Depletion experiments suggest that the absence of one PML-NB component might not affect the recruitment of other components toward DBP oligomers. Thus, our findings suggest a model in which an adenoviral DNA replication factor, but not incoming viral genome complexes, targets and modulates PML-NBs to support a conducive state for viral DNA replication and argue against a generalized concept that PML-NBs target incoming viral genomes. The immediate fate upon nuclear delivery of genomes of incoming DNA viruses is largely unclear. Early reports suggested that incoming genomes of herpesviruses are targeted and repressed by PML-NBs immediately upon nuclear import. Genome localization and/or viral DNA replication has also been observed at PML-NBs for other DNA viruses. Thus, it was suggested that PML-NBs may immediately sense and target nuclear viral genomes and hence serve as sites for deposition of incoming viral genomes and

  5. ANP32B is a nuclear target of henipavirus M proteins.

    Directory of Open Access Journals (Sweden)

    Anja Bauer

    Full Text Available Membrane envelopment and budding of negative strand RNA viruses (NSVs is mainly driven by viral matrix proteins (M. In addition, several M proteins are also known to be involved in host cell manipulation. Knowledge about the cellular targets and detailed molecular mechanisms, however, is poor for many M proteins. For instance, Nipah Virus (NiV M protein trafficking through the nucleus is essential for virus release, but nuclear targets of NiV M remain unknown. To identify cellular interactors of henipavirus M proteins, tagged Hendra Virus (HeV M proteins were expressed and M-containing protein complexes were isolated and analysed. Presence of acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B in the complex suggested that this protein represents a direct or indirect interactor of the viral matrix protein. Over-expression of ANP32B led to specific nuclear accumulation of HeV M, providing a functional link between ANP32B and M protein. ANP32B-dependent nuclear accumulation was observed after plasmid-driven expression of HeV and NiV matrix proteins and also in NiV infected cells. The latter indicated that an interaction of henipavirus M protein with ANP32B also occurs in the context of virus replication. From these data we conclude that ANP32B is a nuclear target of henipavirus M that may contribute to virus replication. Potential effects of ANP32B on HeV nuclear shuttling and host cell manipulation by HeV M affecting ANP32B functions in host cell survival and gene expression regulation are discussed.

  6. Nuclear power unnecessary for climate protection. There are more cost-efficient alternatives

    Energy Technology Data Exchange (ETDEWEB)

    Kemfert, Claudia; Oei, Pao-Yu [DIW Berlin (Germany). Dept. of Energy, Transportation, Environment; Burandt, Thorsten; Hainsch, Karlo [TU Berlin (Germany); Loeffler, Konstantin [TU Berlin (Germany); DIW Berlin (Germany); Hirschhausen, Christian von [DIW Berlin (Germany). International Infrastructure Policy and Industrial Organization

    2017-07-01

    The world needs to continue working to protect the climate - this is generally undisputed. However, there is no agreement on which technologies should be used to decarbonize the energy sector. Many international scenarios still assume a relevant role for nuclear power in the future. However, a study by the German Institute for Economic Research shows that the Paris climate protection target - limiting global warming to below two degrees - can be achieved inexpensively without nuclear power. The results of a global energy system model indicate that no new nuclear power plants have to be built in order to meet the global climate target. It would be cheaper to use a combination of renewable energy and energy storage systems.

  7. EDF decommissioning programme: A global commitment to safety, environment and cost efficiency of nuclear energy

    International Nuclear Information System (INIS)

    Chatry, Jean-Paul

    2002-01-01

    Nowadays, decommissioning of nuclear power plants has become a key issue for nuclear industry in Europe. The phasing out of nuclear energy in Germany, Belgium and Sweden, as well as the early closure of nuclear units in applicant countries in the frame of EU enlargement, has largely contributed to consider decommissioning as the next challenge to face. The situation is slightly different in France: nuclear energy is still considered as a safe, cost-effective and environment friendly energy source and EDF is still working on the development of a new generation of reactor to replace the existing one. Nevertheless, to achieve this objective, it will be necessary to get the support of political decision-makers and the acceptance of public opinion. The increasing mobilisation of EDF for the decommissioning of its already shutdown NPPs shows its willingness to demonstrate its capacity to control the nuclear life cycle from end to end. The successful implementation of its decommissioning programme will not mean the end of nuclear energy as an efficient way to generate electricity but it will constitute a prerequisite for the erection of new nuclear power plants in France

  8. Cratering efficiency on coarse-grain targets: Implications for the dynamical evolution of asteroid 25143 Itokawa

    Science.gov (United States)

    Tatsumi, Eri; Sugita, Seiji

    2018-01-01

    Remote sensing observations made by the spacecraft Hayabusa provided the first direct evidence of a rubble-pile asteroid: 25143 Itokawa. Itokawa was found to have a surface structure very different from other explored asteroids; covered with coarse pebbles and boulders ranging at least from cm to meter size. The cumulative size distribution of small circular depressions on Itokawa, most of which may be of impact origin, has a significantly shallower slope than that on the Moon; small craters are highly depleted on Itokawa compared to the Moon. This deficiency of small circular depressions and other features, such as clustered fragments and pits on boulders, suggest that the boulders on Itokawa might behave like armor, preventing crater formation: the ;armoring effect;. This might contribute to the low number density of small crater candidates. In this study, the cratering efficiency reduction due to coarse-grained targets was investigated based on impact experiments at velocities ranging from ∼ 70 m/s to ∼ 6 km/s using two vertical gas gun ranges. We propose a scaling law extended for cratering on coarse-grained targets (i.e., target grain size ≳ projectile size). We have found that the crater efficiency reduction is caused by energy dissipation at the collision site where momentum is transferred from the impactor to the first-contact target grain, and that the armoring effect can be classified into three regimes: (1) gravity scaled regime, (2) reduced size crater regime, or (3) no apparent crater regime, depending on the ratio of the impactor size to the target grain size and the ratio of the impactor kinetic energy to the disruption energy of a target grain. We found that the shallow slope of the circular depressions on Itokawa cannot be accounted for by this new scaling law, suggesting that obliteration processes, such as regolith convection and migration, play a greater role in the depletion of circular depressions on Itokawa. Based on the new extended

  9. Synthesis of taurine–fluorescein conjugate and evaluation of its retina-targeted efficiency in vitro

    Directory of Open Access Journals (Sweden)

    Meihong Huang

    2014-12-01

    Full Text Available In this work, retinal penetration of fluorescein was achieved in vitro by covalent attachment of taurine to fluorescein, yielding the F–Tau conjugate. Nuclear magnetic resonance (NMR and high resolution mass spectrometry (HRMS were used to confirm the successful synthesis of F–Tau. The cellular uptake of F–Tau in adult retinal pigment epithelial cells (ARPE-19 and human retinal microvascular endothelial cells (hRMECs was visualized via confocal scanning microscopy. The results indicated an improvement of solubility and a reduction of logP of F–Tau compared with fluorescein. As compared with fluorescein, F–Tau showed little toxicity, and was retained longer by cells in uptake experiments. F–Tau also displayed higher transepithelial permeabilities than fluorescein in ARPE-19 and hRMECs monolayer cells (P<0.05. These results showed that taurine may be a useful ligand for targeting small-molecule hydrophobic pharmaceuticals into the retina.

  10. Design of effective energy efficiency policies. An analysis in the frame of target setting, monitoring and evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Schlomann, Barbara

    2014-07-01

    Energy efficiency is widely acknowledged as the most important strategy for achieving global energy and climate targets. Apart from its contribution to the reduction of energy consumption and energy-related greenhouse gas emissions (GHG), improving energy efficiency can deliver a range of co-benefits to the economy and society. There are, however, indications that energy efficiency policy is still insufficiently anchored both in the EU and many Member States as well as at an international level. This thesis focuses on the question how to create more favourable preconditions for an effective anchoring of energy efficiency policy in energy and climate policy. The design of energy efficiency policies is analyzed in the frame of the setting of energy efficiency targets and the monitoring and evaluation of their success. This provides new insights in the functioning of policies and hence their improvement in view of target achievement.

  11. Electron spin resonance and its implication on the maximum nuclear polarization of deuterated solid target materials

    International Nuclear Information System (INIS)

    Heckmann, J.; Meyer, W.; Radtke, E.; Reicherz, G.; Goertz, S.

    2006-01-01

    ESR spectroscopy is an important tool in polarized solid target material research, since it allows us to study the paramagnetic centers, which are used for the dynamic nuclear polarization (DNP). The polarization behavior of the different target materials is strongly affected by the properties of these centers, which are added to the diamagnetic materials by chemical doping or irradiation. In particular, the ESR linewidth of the paramagnetic centers is a very important parameter, especially concerning the deuterated target materials. In this paper, the results of the first precise ESR measurements of the deuterated target materials at a DNP-relevant magnetic field of 2.5 T are presented. Moreover, these results allowed us to experimentally study the correlation between ESR linewidth and maximum deuteron polarization, as given by the spin-temperature theory

  12. Simple and Efficient Targeting of Multiple Genes Through CRISPR-Cas9 in Physcomitrella patens

    Directory of Open Access Journals (Sweden)

    Mauricio Lopez-Obando

    2016-11-01

    Full Text Available Powerful genome editing technologies are needed for efficient gene function analysis. The CRISPR-Cas9 system has been adapted as an efficient gene-knock-out technology in a variety of species. However, in a number of situations, knocking out or modifying a single gene is not sufficient; this is particularly true for genes belonging to a common family, or for genes showing redundant functions. Like many plants, the model organism Physcomitrella patens has experienced multiple events of polyploidization during evolution that has resulted in a number of families of duplicated genes. Here, we report a robust CRISPR-Cas9 system, based on the codelivery of a CAS9 expressing cassette, multiple sgRNA vectors, and a cassette for transient transformation selection, for gene knock-out in multiple gene families. We demonstrate that CRISPR-Cas9-mediated targeting of five different genes allows the selection of a quintuple mutant, and all possible subcombinations of mutants, in one experiment, with no mutations detected in potential off-target sequences. Furthermore, we confirmed the observation that the presence of repeats in the vicinity of the cutting region favors deletion due to the alternative end joining pathway, for which induced frameshift mutations can be potentially predicted. Because the number of multiple gene families in Physcomitrella is substantial, this tool opens new perspectives to study the role of expanded gene families in the colonization of land by plants.

  13. Distributed Bees Algorithm Parameters Optimization for a Cost Efficient Target Allocation in Swarms of Robots

    Directory of Open Access Journals (Sweden)

    Álvaro Gutiérrez

    2011-11-01

    Full Text Available Swarms of robots can use their sensing abilities to explore unknown environments and deploy on sites of interest. In this task, a large number of robots is more effective than a single unit because of their ability to quickly cover the area. However, the coordination of large teams of robots is not an easy problem, especially when the resources for the deployment are limited. In this paper, the Distributed Bees Algorithm (DBA, previously proposed by the authors, is optimized and applied to distributed target allocation in swarms of robots. Improved target allocation in terms of deployment cost efficiency is achieved through optimization of the DBA’s control parameters by means of a Genetic Algorithm. Experimental results show that with the optimized set of parameters, the deployment cost measured as the average distance traveled by the robots is reduced. The cost-efficient deployment is in some cases achieved at the expense of increased robots’ distribution error. Nevertheless, the proposed approach allows the swarm to adapt to the operating conditions when available resources are scarce.

  14. Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9

    Science.gov (United States)

    Li, Minghui; Yang, Huihui; Zhao, Jiue; Fang, Lingling; Shi, Hongjuan; Li, Mengru; Sun, Yunlv; Zhang, Xianbo; Jiang, Dongneng; Zhou, Linyan; Wang, Deshou

    2014-01-01

    Studies of gene function in non-model animals have been limited by the approaches available for eliminating gene function. The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated) system has recently become a powerful tool for targeted genome editing. Here, we report the use of the CRISPR/Cas9 system to disrupt selected genes, including nanos2, nanos3, dmrt1, and foxl2, with efficiencies as high as 95%. In addition, mutations in dmrt1 and foxl2 induced by CRISPR/Cas9 were efficiently transmitted through the germline to F1. Obvious phenotypes were observed in the G0 generation after mutation of germ cell or somatic cell-specific genes. For example, loss of Nanos2 and Nanos3 in XY and XX fish resulted in germ cell-deficient gonads as demonstrated by GFP labeling and Vasa staining, respectively, while masculinization of somatic cells in both XY and XX gonads was demonstrated by Dmrt1 and Cyp11b2 immunohistochemistry and by up-regulation of serum androgen levels. Our data demonstrate that targeted, heritable gene editing can be achieved in tilapia, providing a convenient and effective approach for generating loss-of-function mutants. Furthermore, our study shows the utility of the CRISPR/Cas9 system for genetic engineering in non-model species like tilapia and potentially in many other teleost species. PMID:24709635

  15. Impact of the recession on the realization of the Kyoto target and the 2011 target of the working programme 'Clean and Efficient'. Memo

    International Nuclear Information System (INIS)

    2009-01-01

    By request of the Dutch Ministry of Housing, Spatial Planning and the Environment the Environmental Assessment Agency (PBL) drew up this memo on the impact of the recession on the realization of the Kyoto target and the intermediate target for 2011 from the working programme Clean and Efficient. The main conclusion of this memo is that both with and without the recession the Kyoto target will probably be realized. The main difference lies in the volume of emission allowances deployed by the government to realize the target [mk] [nl

  16. Efficiency improvement of nuclear power plant operation: the significant role of advanced nuclear fuel technologies

    International Nuclear Information System (INIS)

    Van Velde, AA. de; Burtak, F.

    2000-01-01

    In this paper authors deals with nuclear fuel cycle and their economic aspects. At Siemens, the developments focusing on the reduction of fuel cycle costs are currently directed on .further batch average burnup increase, .improvement of fuel reliability, .enlargement of fuel operation margins, .improvement of methods for fuel design and core analysis. These items will be presented in detail in the full paper and illustrated by the global operating experience of Siemens fuel for both PWRs and BWRs. (authors)

  17. Non-statistical fluctuations in fragmentation of target nuclei in high energy nuclear interactions

    Energy Technology Data Exchange (ETDEWEB)

    Ghosh, Dipak; Ghosh, Premomoy; Ghosh, Alokananda; Roy, Jaya [Jadavpur Univ., Calcutta (India)

    1994-07-01

    Analysis of target fragmented ''black'' particles in nuclear emulsion from high energy relativistic interactions initiated by [sup 16]O at 2.1 GeV/nucleon and [sup 12]C and [sup 24]Mg at 4.5 GeV/nucleon reveal the existence of non-statistical fluctuations in the azimuthal plane of interaction. The asymmetry or the non-statistical fluctuations, while found to be independent of projectile mass or incident energy, are dependent on the excitation energy of the target nucleus. (Author).

  18. Non-statistical fluctuations in fragmentation of target nuclei in high energy nuclear interactions

    International Nuclear Information System (INIS)

    Ghosh, Dipak; Ghosh, Premomoy; Ghosh, Alokananda; Roy, Jaya

    1994-01-01

    Analysis of target fragmented ''black'' particles in nuclear emulsion from high energy relativistic interactions initiated by 16 O at 2.1 GeV/nucleon and 12 C and 24 Mg at 4.5 GeV/nucleon reveal the existence of non-statistical fluctuations in the azimuthal plane of interaction. The asymmetry or the non-statistical fluctuations, while found to be independent of projectile mass or incident energy, are dependent on the excitation energy of the target nucleus. (Author)

  19. Hospital structure and technical efficiency in the production of nuclear medicine. Doctoral thesis

    International Nuclear Information System (INIS)

    Wilson, G.W.

    1976-01-01

    The relationship of hospital structure to production efficiency is explored. The hospital subindustry on which this research centers is nuclear medicine. The hypotheses generated were that technical efficiency is reduced by increased competitive intensity, by a lack of profit incentive, by a broader service range, and by in-house training of technical personnel. Most data employed in the study were gathered from the American College of Radiology and the Energy Research and Development Administration Census of Nuclear Medicine. More specific information came from questionnaires sent to 2,050 short-term general hospitals known to have a nuclear medicine facility. Of the responses 1,362 were usable for the study. A major study finding was that over half of the variations observed in technical efficiency were attributable to the structural elements being studied. The research indicated that competition for staff physicians has a role in reducing technical efficiency; that the output effect of in-house manpower training was relatively unimportant; and that profit incentives do have a significant impact. It is suggested that increased technical efficiency could be achieved through reduced competitive intensity, stronger profit orientation, and reduced service range. A bibliography is included

  20. Efficient and Adaptive Node Selection for Target Tracking in Wireless Sensor Network

    Directory of Open Access Journals (Sweden)

    Juan Feng

    2016-01-01

    Full Text Available In target tracking wireless sensor network, choosing the proper working nodes can not only minimize the number of active nodes, but also satisfy the tracking reliability requirement. However, most existing works focus on selecting sensor nodes which are the nearest to the target for tracking missions and they did not consider the correlation of the location of the sensor nodes so that these approaches can not meet all the goals of the network. This work proposes an efficient and adaptive node selection approach for tracking a target in a distributed wireless sensor network. The proposed approach combines the distance-based node selection strategy and particle filter prediction considering the spatial correlation of the different sensing nodes. Moreover, a joint distance weighted measurement is proposed to estimate the information utility of sensing nodes. Experimental results show that EANS outperformed the state-of-the-art approaches by reducing the energy cost and computational complexity as well as guaranteeing the tracking accuracy.

  1. Simulation and Automation of Microwave Frequency Control in Dynamic Nuclear Polarization for Solid Polarized Targets

    Science.gov (United States)

    Perera, Gonaduwage; Johnson, Ian; Keller, Dustin

    2017-09-01

    Dynamic Nuclear Polarization (DNP) is used in most of the solid polarized target scattering experiments. Those target materials must be irradiated using microwaves at a frequency determined by the difference in the nuclear Larmor and electron paramagnetic resonance (EPR) frequencies. But the resonance frequency changes with time as a result of radiation damage. Hence the microwave frequency should be adjusted accordingly. Manually adjusting the frequency can be difficult, and improper adjustments negatively impact the polarization. In order to overcome these difficulties, two controllers were developed which automate the process of seeking and maintaining the optimal frequency: one being a standalone controller for a traditional DC motor and the other a LabVIEW VI for a stepper motor configuration. Further a Monte-Carlo simulation was developed which can accurately model the polarization over time as a function of microwave frequency. In this talk, analysis of the simulated data and recent improvements to the automated system will be presented. DOE.

  2. Nuclear trafficking of proteins from RNA viruses: potential target for antivirals?

    Science.gov (United States)

    Caly, Leon; Wagstaff, Kylie M; Jans, David A

    2012-09-01

    A key aspect of the infectious cycle of many viruses is the transport of specific viral proteins into the host cell nucleus to perturb the antiviral response. Examples include a number of RNA viruses that are significant human pathogens, such as human immunodeficiency virus (HIV)-1, influenza A, dengue, respiratory syncytial virus and rabies, as well agents that predominantly infect livestock, such as Rift valley fever virus and Venezuelan equine encephalitis virus. Inhibiting the nuclear trafficking of viral proteins as a therapeutic strategy offers an attractive possibility, with important recent progress having been made with respect to HIV-1 and dengue. The results validate nuclear protein import as an antiviral target, and suggest the identification and development of nuclear transport inhibitors as a viable therapeutic approach for a range of human and zoonotic pathogenic viruses. Copyright © 2012 Elsevier B.V. All rights reserved.

  3. Relationship among reaction rate, release rate and efficiency of nanomachine-based targeted drug delivery.

    Science.gov (United States)

    Zhao, Qingying; Li, Min; Luo, Jun

    2017-12-04

    In nanomachine applications towards targeted drug delivery, drug molecules released by nanomachines propagate and chemically react with tumor cells in aqueous environment. If the nanomachines release drug molecules faster than the tumor cells react, it will result in loss and waste of drug molecules. It is a potential issue associated with the relationship among reaction rate, release rate and efficiency. This paper aims to investigate the relationship among reaction rate, release rate and efficiency based on two drug reception models. We expect to pave a way for designing a control method of drug release. We adopted two analytical methods that one is drug reception process based on collision with tumors and another is based on Michaelis Menten enzymatic kinetics. To evaluate the analytical formulations, we used the well-known simulation framework N3Sim to establish simulations. The analytical results of the relationship among reaction rate, release rate and efficiency is obtained, which match well with the numerical simulation results in a 3-D environment. Based upon two drug reception models, the results of this paper would be beneficial for designing a control method of nanomahine-based drug release.

  4. Further studies of Fermi-motion effects in lepton scattering from nuclear targets

    International Nuclear Information System (INIS)

    Bodek, A.; Ritchie, J.L.

    1981-01-01

    We have calculated the ratio of deep-inelastic structure functions of nuclear targets to the sum of free-neutron and -proton structure functions. The calculations incorporate structure-function fits which are based on quantum-chromodynamic considerations. This paper is an addendum to an earlier publication in which we calculated the Fermi-motion corrections using other fits to the nucleon structure functions

  5. The influence of target and sample properties on nuclear data measurements

    International Nuclear Information System (INIS)

    Okamoto, K.

    1988-10-01

    The IAEA Advisory Group Meeting (AGM) on The Influence of Target and Sample Properties on Nuclear Data Measurements was held at the Gesellschaft fuer Schwerionenforschung mbH, Darmstadt, Federal Republic of Germany, during the week 5-9 September 1988. The AGM (hereafter ''Meeting'') was held concurrently during the 14th World Conference (hereafter ''Conference'') of the International Nuclear Target Development Society (INTDS) in co-operation with the IAEA-International Nuclear Data Committee (INDC). The Meeting's special sessions (5th, 7th and 9th September 1988) were held to review and prepare the summary of the papers presented to the Conference and recommendations on the objectives of the AGM. The contributed papers to the Conference are to be published in the Journal Nuclear Instruments and Methods in Physical Research. The contributed notes to the Meeting's special sessions together with the summary of the contributed papers by the Agency's invitees and the discussions during the Meeting's special sessions and the recommendations are issued in this report. (author). Refs, figs and tabs

  6. Design and Optimization for the Windowless Target of the China Nuclear Waste Transmutation Reactor

    Directory of Open Access Journals (Sweden)

    Desheng Cheng

    2016-04-01

    Full Text Available A windowless spallation target can provide a neutron source and maintain neutron chain reaction for a subcritical reactor, and is a key component of China's nuclear waste transmutation of coupling accelerator and subcritical reactor. The main issue of the windowless target design is to form a stable and controllable free surface that can ensure that energy spectrum distribution is acquired for the neutron physical design when the high energy proton beam beats the lead–bismuth eutectic in the spallation target area. In this study, morphology and flow characteristics of the free surface of the windowless target were analyzed through the volume of fluid model using computational fluid dynamics simulation, and the results show that the outlet cross section size of the target is the key to form a stable and controllable free surface, as well as the outlet with an arc transition. The optimization parameter of the target design, in which the radius of outlet cross section is 60 ± 1 mm, is verified to form a stable and controllable free surface and to reduce the formation of air bubbles. This work can function as a reference for carrying out engineering design of windowless target and for verification experiments.

  7. RNAi-Based Identification of Gene-Specific Nuclear Cofactor Networks Regulating Interleukin-1 Target Genes

    Directory of Open Access Journals (Sweden)

    Johanna Meier-Soelch

    2018-04-01

    Full Text Available The potent proinflammatory cytokine interleukin (IL-1 triggers gene expression through the NF-κB signaling pathway. Here, we investigated the cofactor requirements of strongly regulated IL-1 target genes whose expression is impaired in p65 NF-κB-deficient murine embryonic fibroblasts. By two independent small-hairpin (shRNA screens, we examined 170 genes annotated to encode nuclear cofactors for their role in Cxcl2 mRNA expression and identified 22 factors that modulated basal or IL-1-inducible Cxcl2 levels. The functions of 16 of these factors were validated for Cxcl2 and further analyzed for their role in regulation of 10 additional IL-1 target genes by RT-qPCR. These data reveal that each inducible gene has its own (quantitative requirement of cofactors to maintain basal levels and to respond to IL-1. Twelve factors (Epc1, H2afz, Kdm2b, Kdm6a, Mbd3, Mta2, Phf21a, Ruvbl1, Sin3b, Suv420h1, Taf1, and Ube3a have not been previously implicated in inflammatory cytokine functions. Bioinformatics analysis indicates that they are components of complex nuclear protein networks that regulate chromatin functions and gene transcription. Collectively, these data suggest that downstream from the essential NF-κB signal each cytokine-inducible target gene has further subtle requirements for individual sets of nuclear cofactors that shape its transcriptional activation profile.

  8. Nuclear Level Densities for Modeling Nuclear Reactions: An Efficient Approach Using Statistical Spectroscopy

    International Nuclear Information System (INIS)

    Calvin W. Johnson

    2005-01-01

    The general goal of the project is to develop and implement computer codes and input files to compute nuclear densities of state. Such densities are important input into calculations of statistical neutron capture, and are difficult to access experimentally. In particular, we will focus on calculating densities for nuclides in the mass range A ∼ 50-100. We use statistical spectroscopy, a moments method based upon a microscopic framework, the interacting shell model. Second year goals and milestones: Develop two or three competing interactions (based upon surface-delta, Gogny, and NN-scattering) suitable for application to nuclei up to A = 100. Begin calculations for nuclides with A = 50-70

  9. Computational Efficient Upscaling Methodology for Predicting Thermal Conductivity of Nuclear Waste forms

    International Nuclear Information System (INIS)

    Li, Dongsheng; Sun, Xin; Khaleel, Mohammad A.

    2011-01-01

    This study evaluated different upscaling methods to predict thermal conductivity in loaded nuclear waste form, a heterogeneous material system. The efficiency and accuracy of these methods were compared. Thermal conductivity in loaded nuclear waste form is an important property specific to scientific researchers, in waste form Integrated performance and safety code (IPSC). The effective thermal conductivity obtained from microstructure information and local thermal conductivity of different components is critical in predicting the life and performance of waste form during storage. How the heat generated during storage is directly related to thermal conductivity, which in turn determining the mechanical deformation behavior, corrosion resistance and aging performance. Several methods, including the Taylor model, Sachs model, self-consistent model, and statistical upscaling models were developed and implemented. Due to the absence of experimental data, prediction results from finite element method (FEM) were used as reference to determine the accuracy of different upscaling models. Micrographs from different loading of nuclear waste were used in the prediction of thermal conductivity. Prediction results demonstrated that in term of efficiency, boundary models (Taylor and Sachs model) are better than self consistent model, statistical upscaling method and FEM. Balancing the computation resource and accuracy, statistical upscaling is a computational efficient method in predicting effective thermal conductivity for nuclear waste form.

  10. High-efficiency optical pumping of nuclear polarization in a GaAs quantum well

    Science.gov (United States)

    Mocek, R. W.; Korenev, V. L.; Bayer, M.; Kotur, M.; Dzhioev, R. I.; Tolmachev, D. O.; Cascio, G.; Kavokin, K. V.; Suter, D.

    2017-11-01

    The dynamic polarization of nuclear spins by photoexcited electrons is studied in a high quality GaAs/AlGaAs quantum well. We find a surprisingly high efficiency of the spin transfer from the electrons to the nuclei as reflected by a maximum nuclear field of 0.9 T in a tilted external magnetic field of 1 T strength only. This high efficiency is due to a low leakage of spin out of the polarized nuclear system, because mechanisms of spin relaxation other than the hyperfine interaction are strongly suppressed, leading to a long nuclear relaxation time of up to 1000 s. A key ingredient to that end is the low impurity concentration inside the heterostructure, while the electrostatic potential from charged impurities in the surrounding barriers becomes screened through illumination by which the spin relaxation time is increased compared to keeping the system in the dark. This finding indicates a strategy for obtaining high nuclear spin polarization as required for long-lasting carrier spin coherence.

  11. Can we improve the identification of cold homes for targeted home energy-efficiency improvements?

    International Nuclear Information System (INIS)

    Hutchinson, Emma J.; Wilkinson, Paul; Hong, Sung H.; Oreszczyn, Tadj

    2006-01-01

    Objective: To investigate the extent to which homes with low indoor-temperatures can be identified from dwelling and household characteristics. Design: Analysis of data from a national survey of dwellings, occupied by low-income households, scheduled for home energy-efficiency improvements. Setting: Five urban areas of England: Birmingham, Liverpool, Manchester, Newcastle and Southampton. Methods: Half-hourly living-room temperatures were recorded for two to four weeks in dwellings over the winter periods November to April 2001-2002 and 2002-2003. Regression of indoor on outdoor temperatures was used to identify cold-homes in which standardized daytime living-room and/or nighttime bedroom-temperatures were o C (when the outdoor temperature was 5 o C). Tabulation and logistic regression were used to examine the extent to which these cold-homes can be identified from dwelling and household characteristics. Results: Overall, 21.0% of dwellings had standardized daytime living-room temperatures o C, and 46.4% had standardized nighttime bedroom-temperatures below the same temperature. Standardized indoor-temperatures were influenced by a wide range of household and dwelling characteristics, but most strongly by the energy efficiency (SAP) rating and by standardized heating costs. However, even using these variables, along with other dwelling and household characteristics in a multi-variable prediction model, it would be necessary to target more than half of all dwellings in our sample to ensure at least 80% sensitivity for identifying dwellings with cold living-room temperatures. An even higher proportion would have to be targeted to ensure 80% sensitivity for identifying dwellings with cold-bedroom temperatures. Conclusion: Property and household characteristics provide only limited potential for identifying dwellings where winter indoor temperatures are likely to be low, presumably because of the multiple influences on home heating, including personal choice and

  12. Radiological Protection and Nuclear Engineering Studies in Multi-MW Target Systems

    Science.gov (United States)

    Luis, Raul Fernandes

    Several innovative projects involving nuclear technology have emerged around the world in recent years, for applications such as spallation neutron sources, accelerator-driven systems for the transmutation of nuclear waste and radioactive ion beam (RIB) production. While the available neutron Wuxes from nuclear reactors did not increase substantially in intensity over the past three decades, the intensities of neutron sources produced in spallation targets have increased steadily, and should continue to do so during the 21st century. Innovative projects like ESS, MYRRHA and EURISOL lie at the forefront of the ongoing pursuit for increasingly bright neutron sources; driven by proton beams with energies up to 2 GeV and intensities up to several mA, the construction of their proposed facilities involves complex Nuclear Technology and Radiological Protection design studies executed by multidisciplinary teams of scientists and engineers from diUerent branches of Science. The intense neutron Wuxes foreseen for those facilities can be used in several scientiVc research Velds, such as Nuclear Physics and Astrophysics, Medicine and Materials Science. In this work, the target systems of two facilitites for the production of RIBs using the Isotope Separation On-Line (ISOL) method were studied in detail: ISOLDE, operating at CERN since 1967, and EURISOL, the next-generation ISOL facility to be built in Europe. For the EURISOL multi-MW target station, a detailed study of Radiological Protection was carried out using the Monte Carlo code FLUKA. Simulations were done to assess neutron Wuences, Vssion rates, ambient dose equivalent rates during operation and after shutdown and the production of radioactive nuclei in the targets and surrounding materials. DiUerent materials were discussed for diUerent components of the target system, aiming at improving its neutronics performance while keeping the residual activities resulting from material activation as low as possible. The second

  13. Integrity of the Linker of Nucleoskeleton and Cytoskeleton Is Required for Efficient Herpesvirus Nuclear Egress.

    Science.gov (United States)

    Klupp, Barbara G; Hellberg, Teresa; Granzow, Harald; Franzke, Kati; Dominguez Gonzalez, Beatriz; Goodchild, Rose E; Mettenleiter, Thomas C

    2017-10-01

    Herpesvirus capsids assemble in the nucleus, while final virion maturation proceeds in the cytoplasm. This requires that newly formed nucleocapsids cross the nuclear envelope (NE), which occurs by budding at the inner nuclear membrane (INM), release of the primary enveloped virion into the perinuclear space (PNS), and subsequent rapid fusion with the outer nuclear membrane (ONM). During this process, the NE remains intact, even at late stages of infection. In addition, the spacing between the INM and ONM is maintained, as is that between the primary virion envelope and nuclear membranes. The linker of nucleoskeleton and cytoskeleton (LINC) complex consists of INM proteins with a luminal SUN (Sad1/UNC-84 homology) domain connected to ONM proteins with a KASH (Klarsicht, ANC-1, SYNE homology) domain and is thought to be responsible for spacing the nuclear membranes. To investigate the role of the LINC complex during herpesvirus infection, we generated cell lines constitutively expressing dominant negative (dn) forms of SUN1 and SUN2. Ultrastructural analyses revealed a significant expansion of the PNS and the contiguous intracytoplasmic lumen, most likely representing endoplasmic reticulum (ER), especially in cells expressing dn-SUN2. After infection, primary virions accumulated in these expanded luminal regions, also very distant from the nucleus. The importance of the LINC complex was also confirmed by reduced progeny virus titers in cells expressing dn-SUN2. These data show that the intact LINC complex is required for efficient nuclear egress of herpesviruses, likely acting to promote fusion of primary enveloped virions with the ONM. IMPORTANCE While the viral factors for primary envelopment of nucleocapsids at the inner nuclear membrane are known to the point of high-resolution structures, the roles of cellular components and regulators remain enigmatic. Furthermore, the machinery responsible for fusion with the outer nuclear membrane is unsolved. We show here

  14. International Experience with Key Program Elements of IndustrialEnergy Efficiency or Greenhouse Gas Emissions Reduction Target-SettingPrograms

    Energy Technology Data Exchange (ETDEWEB)

    Price, Lynn; Galitsky, Christina; Kramer, Klaas Jan

    2008-02-02

    Target-setting agreements, also known as voluntary ornegotiated agreements, have been used by a number of governments as amechanism for promoting energy efficiency within the industrial sector. Arecent survey of such target-setting agreement programs identified 23energy efficiency or GHG emissions reduction voluntary agreement programsin 18 countries. International best practice related to target-settingagreement programs calls for establishment of a coordinated set ofpolicies that provide strong economic incentives as well as technical andfinancial support to participating industries. The key program elementsof a target-setting program are the target-setting process,identification of energy-saving technologies and measures usingenergy-energy efficiency guidebooks and benchmarking as well as byconducting energy-efficiency audits, development of an energy-savingsaction plan, development and implementation of energy managementprotocols, development of incentives and supporting policies, monitoringprogress toward targets, and program evaluation. This report firstprovides a description of three key target-setting agreement programs andthen describes international experience with the key program elementsthat comprise such programs using information from the three keytarget-setting programs as well as from other international programsrelated to industrial energy efficiency or GHG emissionsreductions.

  15. Common Risk Target for severe accidents of nuclear power plants based on IAEA INES scale

    International Nuclear Information System (INIS)

    Vitázková, Jiřina; Cazzoli, Errico

    2013-01-01

    The IAEA has repeatedly recommended that the nuclear community should arrive at a common understanding and definition of safety goals for severe accidents in nuclear power plants. The recommendation has only found partial answers, despite the numerous working groups and forums devoted to this effort. The most widely accepted definition of goals is based on the concept of Large (Early) Release Frequencies (L(E)RF) and its derivatives, a surrogate concept derived from results of Probabilistic Safety Assessments (PSAs) which was first introduced in the USA almost twenty years ago and much later accepted by the USNRC for risk informed decision making, but not for safety demonstrations. Other types of Safety Goals have been adopted by some nuclear authorities, but the main drawback of all current definitions is that they may apply only to LWRs. The lack of unifying safety/risk parameter throughout of PSAs worldwide is the basis of the present work, and an attempt is made to arrive at the definition of a Risk Target for severe accidents in NPPs, consistent with the IAEA definitions having a technical basis, which can be adopted without modifications for Generation IV power plants. The proposal of Common Risk Target in this work represents an attempt to define a Common Risk Target based on technical reasoning, reflecting IAEA definitions as well as harmonization requirements raised by the whole European Community in various OECD, ASAMPSA2 and SARNET (Guentay et al., 2006) conclusions and Council Directive of The European Union (Community Framework, 2009) as well as lastly performed stress tests of nuclear power plants throughout the Europe (Peer Review Report, 2012). The basic concept of CRT was first introduced and developed within the European project ASAMPSA2 by the authors of this article and was accepted by majority of world PSA experts participating in final evaluation and survey of the project (Guentay, 2011). In the proposed Risk Target concept an innovative

  16. Common Risk Target for severe accidents of nuclear power plants based on IAEA INES scale

    Energy Technology Data Exchange (ETDEWEB)

    Vitázková, Jiřina, E-mail: jirina@snus.sk [Vitázková-Vitty, Sládkovičova 24, 900 28 Ivanka pri Dunaji (Slovakia); Cazzoli, Errico, E-mail: erik.cazzoli@gmx.net [Cazzoli Consulting, Wiesenweg 14, CH-5415 Nussbaumen (Switzerland)

    2013-09-15

    The IAEA has repeatedly recommended that the nuclear community should arrive at a common understanding and definition of safety goals for severe accidents in nuclear power plants. The recommendation has only found partial answers, despite the numerous working groups and forums devoted to this effort. The most widely accepted definition of goals is based on the concept of Large (Early) Release Frequencies (L(E)RF) and its derivatives, a surrogate concept derived from results of Probabilistic Safety Assessments (PSAs) which was first introduced in the USA almost twenty years ago and much later accepted by the USNRC for risk informed decision making, but not for safety demonstrations. Other types of Safety Goals have been adopted by some nuclear authorities, but the main drawback of all current definitions is that they may apply only to LWRs. The lack of unifying safety/risk parameter throughout of PSAs worldwide is the basis of the present work, and an attempt is made to arrive at the definition of a Risk Target for severe accidents in NPPs, consistent with the IAEA definitions having a technical basis, which can be adopted without modifications for Generation IV power plants. The proposal of Common Risk Target in this work represents an attempt to define a Common Risk Target based on technical reasoning, reflecting IAEA definitions as well as harmonization requirements raised by the whole European Community in various OECD, ASAMPSA2 and SARNET (Guentay et al., 2006) conclusions and Council Directive of The European Union (Community Framework, 2009) as well as lastly performed stress tests of nuclear power plants throughout the Europe (Peer Review Report, 2012). The basic concept of CRT was first introduced and developed within the European project ASAMPSA2 by the authors of this article and was accepted by majority of world PSA experts participating in final evaluation and survey of the project (Guentay, 2011). In the proposed Risk Target concept an innovative

  17. MicroRNA let-7b regulates neural stem cell proliferation and differentiation by targeting nuclear receptor TLX signaling.

    Science.gov (United States)

    Zhao, Chunnian; Sun, GuoQiang; Li, Shengxiu; Lang, Ming-Fei; Yang, Su; Li, Wendong; Shi, Yanhong

    2010-02-02

    Neural stem cell self-renewal and differentiation is orchestrated by precise control of gene expression involving nuclear receptor TLX. Let-7b, a member of the let-7 microRNA family, is expressed in mammalian brains and exhibits increased expression during neural differentiation. However, the role of let-7b in neural stem cell proliferation and differentiation remains unknown. Here we show that let-7b regulates neural stem cell proliferation and differentiation by targeting the stem cell regulator TLX and the cell cycle regulator cyclin D1. Overexpression of let-7b led to reduced neural stem cell proliferation and increased neural differentiation, whereas antisense knockdown of let-7b resulted in enhanced proliferation of neural stem cells. Moreover, in utero electroporation of let-7b to embryonic mouse brains led to reduced cell cycle progression in neural stem cells. Introducing an expression vector of Tlx or cyclin D1 that lacks the let-7b recognition site rescued let-7b-induced proliferation deficiency, suggesting that both TLX and cyclin D1 are important targets for let-7b-mediated regulation of neural stem cell proliferation. Let-7b, by targeting TLX and cyclin D1, establishes an efficient strategy to control neural stem cell proliferation and differentiation.

  18. Efficient energy absorption of intense ps-laser pulse into nanowire target

    Energy Technology Data Exchange (ETDEWEB)

    Habara, H.; Honda, S.; Katayama, M.; Tanaka, K. A. [Graduate School of Engineering, Osaka University, 2-1 Suita, Osaka 565-0871 (Japan); Sakagami, H. [National Institute for Fusion Science, Toki, Gifu 509-5292 (Japan); Nagai, K. [Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuda 4259, Midori-ku, Yokohama 226-8503, Kanagawa (Japan)

    2016-06-15

    The interaction between ultra-intense laser light and vertically aligned carbon nanotubes is investigated to demonstrate efficient laser-energy absorption in the ps laser-pulse regime. Results indicate a clear enhancement of the energy conversion from laser to energetic electrons and a simultaneously small plasma expansion on the surface of the target. A two-dimensional plasma particle calculation exhibits a high absorption through laser propagation deep into the nanotube array, even for a dense array whose structure is much smaller than the laser wavelength. The propagation leads to the radial expansion of plasma perpendicular to the nanotubes rather than to the front side. These features may contribute to fast ignition in inertial confinement fusion and laser particle acceleration, both of which require high current and small surface plasma simultaneously.

  19. An ion accelerator facility for the preparation of nuclear bombardement targets

    International Nuclear Information System (INIS)

    Grime, G.W.; Takacs, J.

    1981-01-01

    As a result of the demand for increasingly complex nuclear bombardment targets in this laboratory, work has started on the construction of a medium-energy accelerator facility capable of preparing targets both by ion implantation and by heavy-ion sputtering. Basic consideration was given in the design to flexibility and simplicity. The ion source chosen was the Harwell sputter ion gun which is capable of producing ions of practically any element at currents up to several hundred μA. This was modified to suit our specific requirement. The acceleration system was constructed to operate at a maximum of 100 kV, and the beam is focussed by a three-cylinder electrostatic lens. The ions are analysed by 50 0 magnet which is capable of a mass dispersion of 7 mm in the target chamber between adjacent mass numbers at mass 100. A slit feedback system is used to stabilise the energy against short-term fluctuations. The system is fitted with two target chambers; one after the magnet and one after the electrostatic lens. The latter is used for applications such as sputtering. Two dimensional scanning is available in both target chambers for ensuring uniformity of implantation over areas larger than the spot size. Using this apparatus, implanted targets of 3 He and 20 Ne have been prepared. In addition high quality films of refractory metals have been sputtered using Ar or Xe beams. (orig.)

  20. International target values 2010 for achievable measurement uncertainties in nuclear material accountancy

    International Nuclear Information System (INIS)

    Dias, Fabio C.; Almeida, Silvio G. de; Renha Junior, Geraldo

    2011-01-01

    The International Target Values (ITVs) are reasonable uncertainty estimates that can be used in judging the reliability of measurement techniques applied to industrial nuclear and fissile materials subject to accountancy and/or safeguards verification. In the absence of relevant experimental estimates, ITVs can also be used to select measurement techniques and calculate sample population during the planning phase of verification activities. It is important to note that ITVs represent estimates of the 'state-of-the-practice', which should be achievable under routine measurement conditions affecting both facility operators and safeguards inspectors, not only in the field, but also in laboratory. Tabulated values cover measurement methods used for the determination of volume or mass of the nuclear material, for its elemental and isotopic assays, and for its sampling. The 2010 edition represents the sixth revision of the International Target Values (ITVs), issued by the International Atomic Energy Agency (IAEA) as a Safeguards Technical Report (STR-368). The first version was released as 'Target Values' in 1979 by the Working Group on Techniques and Standards for Destructive Analysis (WGDA) of the European Safeguards Research and Development Association (ESARDA) and focused on destructive analytical methods. In the latest 2010 revision, international standards in estimating and expressing uncertainties have been considered while maintaining a format that allows comparison with the previous editions of the ITVs. Those standards have been usually applied in QC/QA programmes, as well as qualification of methods, techniques and instruments. Representatives of the Brazilian Nuclear Energy Commission (CNEN) and the Brazilian-Argentine Agency for Accounting and Control of Nuclear Materials (ABACC) participated in previous Consultants Group Meetings since the one convened to establish the first list of ITVs released in 1993 and in subsequent revisions, including the latest one

  1. Design of Effective Energy Efficiency Policies : An analysis in the frame of target setting, monitoring and evaluation

    NARCIS (Netherlands)

    Schlomann, B.

    2014-01-01

    Energy efficiency (EE) is widely acknowledged as the most important strategy for achieving global energy and climate targets. Apart from its contribution to the reduction of energy consumption and energy-related greenhouse gas emissions (GHG), improving energy efficiency can deliver a range of

  2. Nonmyopic Sensor Scheduling and its Efficient Implementation for Target Tracking Applications

    Directory of Open Access Journals (Sweden)

    Morrell Darryl

    2006-01-01

    Full Text Available We propose two nonmyopic sensor scheduling algorithms for target tracking applications. We consider a scenario where a bearing-only sensor is constrained to move in a finite number of directions to track a target in a two-dimensional plane. Both algorithms provide the best sensor sequence by minimizing a predicted expected scheduler cost over a finite time-horizon. The first algorithm approximately computes the scheduler costs based on the predicted covariance matrix of the tracker error. The second algorithm uses the unscented transform in conjunction with a particle filter to approximate covariance-based costs or information-theoretic costs. We also propose the use of two branch-and-bound-based optimal pruning algorithms for efficient implementation of the scheduling algorithms. We design the first pruning algorithm by combining branch-and-bound with a breadth-first search and a greedy-search; the second pruning algorithm combines branch-and-bound with a uniform-cost search. Simulation results demonstrate the advantage of nonmyopic scheduling over myopic scheduling and the significant savings in computational and memory resources when using the pruning algorithms.

  3. Adaptive Energy-Efficient Target Detection Based on Mobile Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Tengyue Zou

    2017-05-01

    Full Text Available Target detection is a widely used application for area surveillance, elder care, and fire alarms; its purpose is to find a particular object or event in a region of interest. Usually, fixed observing stations or static sensor nodes are arranged uniformly in the field. However, each part of the field has a different probability of being intruded upon; if an object suddenly enters an area with few guardian devices, a loss of detection will occur, and the stations in the safe areas will waste their energy for a long time without any discovery. Thus, mobile wireless sensor networks may benefit from adaptation and pertinence in detection. Sensor nodes equipped with wheels are able to move towards the risk area via an adaptive learning procedure based on Bayesian networks. Furthermore, a clustering algorithm based on k-means++ and an energy control mechanism is used to reduce the energy consumption of nodes. The extended Kalman filter and a voting data fusion method are employed to raise the localization accuracy of the target. The simulation and experimental results indicate that this new system with adaptive energy-efficient methods is able to achieve better performance than the traditional ones.

  4. Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease

    Science.gov (United States)

    Flowers, G. Parker; Timberlake, Andrew T.; Mclean, Kaitlin C.; Monaghan, James R.; Crews, Craig M.

    2014-01-01

    Among tetrapods, only urodele salamanders, such as the axolotl Ambystoma mexicanum, can completely regenerate limbs as adults. The mystery of why salamanders, but not other animals, possess this ability has for generations captivated scientists seeking to induce this phenomenon in other vertebrates. Although many recent advances in molecular biology have allowed limb regeneration and tissue repair in the axolotl to be investigated in increasing detail, the molecular toolkit for the study of this process has been limited. Here, we report that the CRISPR-Cas9 RNA-guided nuclease system can efficiently create mutations at targeted sites within the axolotl genome. We identify individual animals treated with RNA-guided nucleases that have mutation frequencies close to 100% at targeted sites. We employ this technique to completely functionally ablate EGFP expression in transgenic animals and recapitulate developmental phenotypes produced by loss of the conserved gene brachyury. Thus, this advance allows a reverse genetic approach in the axolotl and will undoubtedly provide invaluable insight into the mechanisms of salamanders' unique regenerative ability. PMID:24764077

  5. Adaptive Energy-Efficient Target Detection Based on Mobile Wireless Sensor Networks.

    Science.gov (United States)

    Zou, Tengyue; Li, Zhenjia; Li, Shuyuan; Lin, Shouying

    2017-05-04

    Target detection is a widely used application for area surveillance, elder care, and fire alarms; its purpose is to find a particular object or event in a region of interest. Usually, fixed observing stations or static sensor nodes are arranged uniformly in the field. However, each part of the field has a different probability of being intruded upon; if an object suddenly enters an area with few guardian devices, a loss of detection will occur, and the stations in the safe areas will waste their energy for a long time without any discovery. Thus, mobile wireless sensor networks may benefit from adaptation and pertinence in detection. Sensor nodes equipped with wheels are able to move towards the risk area via an adaptive learning procedure based on Bayesian networks. Furthermore, a clustering algorithm based on k -means++ and an energy control mechanism is used to reduce the energy consumption of nodes. The extended Kalman filter and a voting data fusion method are employed to raise the localization accuracy of the target. The simulation and experimental results indicate that this new system with adaptive energy-efficient methods is able to achieve better performance than the traditional ones.

  6. EU 2030 targets 'unachievable' without long-term nuclear operation

    Energy Technology Data Exchange (ETDEWEB)

    Mitev, Lubomir [NucNet, Brussels (Belgium)

    2015-01-15

    Nuclear energy will continue to support greenhouse gas emission reduction targets until 2020, but without decisions on long-term operation of ageing reactors, it will be difficult for the EU to meet its 2030 targets, International Energy Agency (IEA) executive director Maria van der Hoeven, tells NucNet in an interview. The IEA has quite a few remarks and questions related to the EU goals of competitiveness, security of supply and sustainability. It is good to have these targets, but up until now the EU is missing the direct connection between the three goals. What is mostly needed to achieve the goals is to finalise the EU's internal energy market. Secondly cost-effective climate and energy policies are needed because it is not only about climate and energy, but also about economic development and competitiveness. The ageing EU reactor fleet requires country-level and owner/operator-level decisions in the short term regarding plant safety regulations, plant upgrades, uprates, lifetime extensions and licence renewals. Upgrading and uprating existing nuclear plants is one of the cheapest ways of producing carbon-free electricity in the EU. Without long-term operation, the IEA expects nuclear capacity in the EU could fall by a factor of six by 2030 and that will make it more difficult to achieve the EU's 2030 climate targets. Public opinion is an important topic for the acceptance of all energy sources and it is different in all IEA member countries. Europe is very sensitive to almost all forms of energy, including wind turbines and solar panels. This is linked to a lack of information, so we need more and better transparency on information for people.

  7. An efficient hybrid technique in RCS predictions of complex targets at high frequencies

    Science.gov (United States)

    Algar, María-Jesús; Lozano, Lorena; Moreno, Javier; González, Iván; Cátedra, Felipe

    2017-09-01

    Most computer codes in Radar Cross Section (RCS) prediction use Physical Optics (PO) and Physical theory of Diffraction (PTD) combined with Geometrical Optics (GO) and Geometrical Theory of Diffraction (GTD). The latter approaches are computationally cheaper and much more accurate for curved surfaces, but not applicable for the computation of the RCS of all surfaces of a complex object due to the presence of caustic problems in the analysis of concave surfaces or flat surfaces in the far field. The main contribution of this paper is the development of a hybrid method based on a new combination of two asymptotic techniques: GTD and PO, considering the advantages and avoiding the disadvantages of each of them. A very efficient and accurate method to analyze the RCS of complex structures at high frequencies is obtained with the new combination. The proposed new method has been validated comparing RCS results obtained for some simple cases using the proposed approach and RCS using the rigorous technique of Method of Moments (MoM). Some complex cases have been examined at high frequencies contrasting the results with PO. This study shows the accuracy and the efficiency of the hybrid method and its suitability for the computation of the RCS at really large and complex targets at high frequencies.

  8. A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein.

    Science.gov (United States)

    Majer, Eszter; Navarro, José-Antonio; Daròs, José-Antonio

    2015-09-01

    Plant virus-based expression systems allow quick and efficient production of recombinant proteins in plant biofactories. Among them, a system derived from tobacco etch virus (TEV; genus potyvirus) permits coexpression of equimolar amounts of several recombinant proteins. This work analyzed how to target recombinant proteins to different subcellular localizations in the plant cell using this system. We constructed TEV clones in which green fluorescent protein (GFP), with a chloroplast transit peptide (cTP), a nuclear localization signal (NLS) or a mitochondrial targeting peptide (mTP) was expressed either as the most amino-terminal product or embedded in the viral polyprotein. Results showed that cTP and mTP mediated efficient translocation of GFP to the corresponding organelle only when present at the amino terminus of the viral polyprotein. In contrast, the NLS worked efficiently at both positions. Viruses expressing GFP in the amino terminus of the viral polyprotein produced milder symptoms. Untagged GFPs and cTP and NLS tagged amino-terminal GFPs accumulated to higher amounts in infected tissues. Finally, viral progeny from clones with internal GFPs maintained the extra gene better. These observations will help in the design of potyvirus-based vectors able to coexpress several proteins while targeting different subcellular localizations, as required in plant metabolic engineering. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. The role of nuclear energy in the more efficient exploitation of fossil fuel resources

    International Nuclear Information System (INIS)

    Seifritz, W.

    1978-01-01

    The energy theory of value, being a valuable addition to the debate on the rational exploitation of man's energy reserves, is applied in order to clarify the presently confused energy input/output relations for nuclear and solar systems as they interact with fossil fuel. It is shown on the basis of purely energetics considerations that the nuclear route - at present and in future - is a very efficient way to stretch out and finally to substitute for the limited fossil fuel resources. This is particularly true if one considers the transitory phase where the substituting process has to exhibit a rapid exponential growth rate. The energetical effectiveness of the production of a synthetic fuel, as for example hydrogen by water splitting processes, is addressed at the end and serves to give an idea how effectively the energy available in fossil fuels can be amplified by virtue of the coupling of nuclear energy into the process. (author)

  10. Influence of nuclear cross section data at efficiency calculation of the 3He semiconductor neutron spectrometer

    International Nuclear Information System (INIS)

    Avdic, S.; Pesic, M.

    1992-01-01

    The ORTEC 580 Neutron Spectrometer system contains a detector unit in diode coincidence arrangement for measurement of fast neutron spectrum in the energy range from 1 MeV to 14 MeV. Numerical code HE3 for computation of semiconductor 3 He detector efficiency in a collimated neutron beam is based on analytical method in infinite diode approximation and Monte Carlo method for real spectrometer geometry. Calculations are performed in the first collision approximation in the detector active volume including evaluation of correction factors. Accuracy of relative detector efficiency calculation is improved by using neutron cross section from nuclear library ENDF/B-6. (author)

  11. The importance of servicing for the safety and economic efficiency of nuclear installations

    International Nuclear Information System (INIS)

    Wild, E.

    1991-01-01

    Experience-oriented preventive maintenance is the central and decisive instrument for plant safety. Preventive maintenance, in the overriding sense of the entire system consisting of man and machine, is identical with service. Such service must be readily available, efficient, purposeful, innovative and of high quality. The high quality of German nuclear power plants and their reliability are the result of a constructive cooperation between producer, supplier, service enterprise and operator. (DG) [de

  12. Modernisation of the Olkiluoto nuclear power plant increases the power production efficiency under safe limits

    International Nuclear Information System (INIS)

    Valkeapaeae, R.

    1995-01-01

    Teollisuuden Voima Oy published the efficiency increment plans as a part of the modernisation of the Olkiluoto nuclear power plant. The power of the reactor units, originally designed for 660 MW will now be increased for a second time. The former improvements were made in 1994. The power of the units was increased to 710 MW. After this new renovation the power of the both units will be 830-840 MW. (2 figs.)

  13. The efficiency and fidelity of the in-core nuclear fuel management code FORMOSA-P

    International Nuclear Information System (INIS)

    Kropaczek, D.J.; Turinsky, P.J.

    1994-01-01

    The second-order generalized perturbation theory (GPT), nodal neutronic model utilized within the nuclear fuel management optimization code FORMOSA-P is presented within the context of prediction fidelity and computational efficiency versus forward solution. Key features of thr GPT neutronics model as implemented within the Simulated Annealing optimization adaptive control algorithm are discussed. Supporting results are then presented demonstrating the superior consistency of adaptive control for both global and local optimization searches. (authors). 15 refs., 1 fig., 4 tabs

  14. Efficiency and cost advantages of an advanced-technology nuclear electrolytic hydrogen-energy production facility

    Science.gov (United States)

    Donakowski, T. D.; Escher, W. J. D.; Gregory, D. P.

    1977-01-01

    The concept of an advanced-technology (viz., 1985 technology) nuclear-electrolytic water electrolysis facility was assessed for hydrogen production cost and efficiency expectations. The facility integrates (1) a high-temperature gas-cooled nuclear reactor (HTGR) operating a binary work cycle, (2) direct-current (d-c) electricity generation via acyclic generators, and (3) high-current-density, high-pressure electrolyzers using a solid polymer electrolyte (SPE). All subsystems are close-coupled and optimally interfaced for hydrogen production alone (i.e., without separate production of electrical power). Pipeline-pressure hydrogen and oxygen are produced at 6900 kPa (1000 psi). We found that this advanced facility would produce hydrogen at costs that were approximately half those associated with contemporary-technology nuclear electrolysis: $5.36 versus $10.86/million Btu, respectively. The nuclear-heat-to-hydrogen-energy conversion efficiency for the advanced system was estimated as 43%, versus 25% for the contemporary system.

  15. Preparation of a primary target for the production of fission products in a nuclear reactor

    International Nuclear Information System (INIS)

    Arino, H.; Cosolito, F.J.; George, K.D.; Thornton, A.K.

    1976-01-01

    A primary target for the production of fission products in a nuclear reactor, such as uranium or plutonium fission products, is comprised of an enclosed, cylindrical vessel, preferably comprised of stainless steel, having a thin, continuous, uniform layer of fissionable material, integrally bonded to its inner walls and a port permitting access to the interior of the vessel. A process is also provided for depositing uranium material on to the inner walls of the vessel. Upon irradiation of the target with neutrons from a nuclear reactor, radioactive fission products, such as molybdenum-99, are formed, and thereafter separated from the target by the introduction of an acidic solution through the port to dissolve the irradiated inner layer. The irradiation and dissolution are thus effected in the same vessel without the necessity of transferring the fissionable material and fission products to a separate chemical reactor. Subsequently, the desired isotopes are extracted and purified. Molybdenum-99 decays to technetium-99m which is a valuable medical diagnostic radioisotope. 3 claims, 1 drawing figure

  16. Targeted disruption of Ataxia-telangiectasia mutated gene in miniature pigs by somatic cell nuclear transfer

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young June; Ahn, Kwang Sung; Kim, Minjeong; Kim, Min Ju; Park, Sang-Min; Ryu, Junghyun; Ahn, Jin Seop; Heo, Soon Young; Kang, Jee Hyun; Choi, You Jung [Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan (Korea, Republic of); Choi, Seong-Jun [Institute of Tissue Regeneration Engineering, Dankook University, Cheonan (Korea, Republic of); Shim, Hosup, E-mail: shim@dku.edu [Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan (Korea, Republic of); Institute of Tissue Regeneration Engineering, Dankook University, Cheonan (Korea, Republic of); Department of Physiology, Dankook University School of Medicine, Cheonan (Korea, Republic of)

    2014-10-03

    Highlights: • ATM gene-targeted pigs were produced by somatic cell nuclear transfer. • A novel large animal model for ataxia telangiectasia was developed. • The new model may provide an alternative to the mouse model. - Abstract: Ataxia telangiectasia (A-T) is a recessive autosomal disorder associated with pleiotropic phenotypes, including progressive cerebellar degeneration, gonad atrophy, and growth retardation. Even though A-T is known to be caused by the mutations in the Ataxia telangiectasia mutated (ATM) gene, the correlation between abnormal cellular physiology caused by ATM mutations and the multiple symptoms of A-T disease has not been clearly determined. None of the existing ATM mouse models properly reflects the extent to which neurological degeneration occurs in human. In an attempt to provide a large animal model for A-T, we produced gene-targeted pigs with mutations in the ATM gene by somatic cell nuclear transfer. The disrupted allele in the ATM gene of cloned piglets was confirmed via PCR and Southern blot analysis. The ATM gene-targeted pigs generated in the present study may provide an alternative to the current mouse model for the study of mechanisms underlying A-T disorder and for the development of new therapies.

  17. The Human Nuclear Exosome Targeting Complex Is Loaded onto Newly Synthesized RNA to Direct Early Ribonucleolysis

    Directory of Open Access Journals (Sweden)

    Michal Lubas

    2015-01-01

    Full Text Available The RNA exosome complex constitutes the major nuclear eukaryotic 3′-5′ exonuclease. Outside of nucleoli, the human nucleoplasmic exosome is directed to some of its substrates by the nuclear exosome targeting (NEXT complex. How NEXT targets RNA has remained elusive. Using an in vivo crosslinking approach, we report global RNA binding sites of RBM7, a key component of NEXT. RBM7 associates broadly with RNA polymerase II-derived RNA, including pre-mRNA and short-lived exosome substrates such as promoter upstream transcripts (PROMPTs, enhancer RNAs (eRNAs, and 3′-extended products from snRNA and replication-dependent histone genes. Within pre-mRNA, RBM7 accumulates at the 3′ ends of introns, and pulse-labeling experiments demonstrate that RBM7/NEXT defines an early exosome-targeting pathway for 3′-extended snoRNAs derived from such introns. We propose that RBM7 is generally loaded onto newly synthesized RNA to accommodate exosome action in case of available unprotected RNA 3′ ends.

  18. Targeted disruption of Ataxia-telangiectasia mutated gene in miniature pigs by somatic cell nuclear transfer

    International Nuclear Information System (INIS)

    Kim, Young June; Ahn, Kwang Sung; Kim, Minjeong; Kim, Min Ju; Park, Sang-Min; Ryu, Junghyun; Ahn, Jin Seop; Heo, Soon Young; Kang, Jee Hyun; Choi, You Jung; Choi, Seong-Jun; Shim, Hosup

    2014-01-01

    Highlights: • ATM gene-targeted pigs were produced by somatic cell nuclear transfer. • A novel large animal model for ataxia telangiectasia was developed. • The new model may provide an alternative to the mouse model. - Abstract: Ataxia telangiectasia (A-T) is a recessive autosomal disorder associated with pleiotropic phenotypes, including progressive cerebellar degeneration, gonad atrophy, and growth retardation. Even though A-T is known to be caused by the mutations in the Ataxia telangiectasia mutated (ATM) gene, the correlation between abnormal cellular physiology caused by ATM mutations and the multiple symptoms of A-T disease has not been clearly determined. None of the existing ATM mouse models properly reflects the extent to which neurological degeneration occurs in human. In an attempt to provide a large animal model for A-T, we produced gene-targeted pigs with mutations in the ATM gene by somatic cell nuclear transfer. The disrupted allele in the ATM gene of cloned piglets was confirmed via PCR and Southern blot analysis. The ATM gene-targeted pigs generated in the present study may provide an alternative to the current mouse model for the study of mechanisms underlying A-T disorder and for the development of new therapies

  19. Targeting for energy efficiency and improved energy collaboration between different companies using total site analysis (TSA)

    International Nuclear Information System (INIS)

    Hackl, Roman; Andersson, Eva; Harvey, Simon

    2011-01-01

    Rising fuel prices, increasing costs associated with emissions of green house gases and the threat of global warming make efficient use of energy more and more important. Industrial clusters have the potential to significantly increase energy efficiency by energy collaboration. In this paper Sweden's largest chemical cluster is analysed using the total site analysis (TSA) method. TSA delivers targets for the amount of utility consumed and generated through excess energy recovery by the different processes. The method enables investigation of opportunities to deliver waste heat from one process to another using a common utility system. The cluster consists of 5 chemical companies producing a variety of products, including polyethylene (PE), polyvinyl chloride (PVC), amines, ethylene, oxygen/nitrogen and plasticisers. The companies already work together by exchanging material streams. In this study the potential for energy collaboration is analysed in order to reach an industrial symbiosis. The overall heating and cooling demands of the site are around 442 MW and 953 MW, respectively. 122 MW of heat is produced in boilers and delivered to the processes. TSA is used to stepwise design a site-wide utility system which improves energy efficiency. It is shown that heat recovery in the cluster can be increased by 129 MW, i.e. the current utility demand could be completely eliminated and further 7 MW excess steam can be made available. The proposed retrofitted utility system involves the introduction of a site-wide hot water circuit, increased recovery of low pressure steam and shifting of heating steam pressure to lower levels in a number heat exchangers when possible. Qualitative evaluation of the suggested measures shows that 60 MW of the savings potential could to be achieved with moderate changes to the process utility system corresponding to 50% of the heat produced from purchased fuel in the boilers of the cluster. Further analysis showed that after implementation

  20. High energy nuclear collisions in the few GeV/nucleon region: projectile and target fragmentation

    International Nuclear Information System (INIS)

    Schroeder, L.S.

    1980-06-01

    A general review of nucleon-nucleus and nucleus-nucleus collisions for incident energies <10 GeV/nucleon is presented. The division of these interactions into peripheral and central collisions is briefly discussed. Subjects treated include the following: target and projectile fragmentation systematics, production of exotic nuclear fragments, studies of multiparticle final states, total cross section measurements, results from an experiment that indicate the production of projectile fragments with an anomalously short reaction mean free path, high-energy particle production at backward angles beyond simple N-N kinematic limits, and recent results on backward particle emission in studies with the Berkeley streamer chamber. Both the particle and nuclear physics aspects that are present are considered. A brief discussion of future trends in this energy range ends the presentation. 65 references, 37 figures

  1. The impact of interface bonding efficiency on high-burnup spent nuclear fuel dynamic performance

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Hao, E-mail: jiangh@ornl.gov; Wang, Jy-An John; Wang, Hong

    2016-12-01

    Highlights: • To investigate the impact of interfacial bonding efficiency at pellet-pellet and pellet-clad interfaces of high-burnup (HBU) spent nuclear fuel (SNF) on its dynamic performance. • Flexural rigidity, EI = M/κ, estimated from FEA results were benchmarked with SNF dynamic experimental results, and used to evaluate interface bonding efficiency. • Interface bonding efficiency can significantly dictate the SNF system rigidity and the associated dynamic performance. • With consideration of interface bonding efficiency and fuel cracking, HBU SNF fuel property was estimated with SNF static and dynamic experimental data. - Abstract: Finite element analysis (FEA) was used to investigate the impact of interfacial bonding efficiency at pellet-pellet and pellet-clad interfaces of high-burnup (HBU) spent nuclear fuel (SNF) on system dynamic performance. Bending moments M were applied to FEA model to evaluate the system responses. From bending curvature, κ, flexural rigidity EI can be estimated as EI = M/κ. The FEA simulation results were benchmarked with experimental results from cyclic integrated reversal bending fatigue test (CIRFT) of HBR fuel rods. The consequence of interface debonding between fuel pellets and cladding is a redistribution of the loads carried by the fuel pellets to the clad, which results in a reduction in composite rod system flexural rigidity. Therefore, the interface bonding efficiency at the pellet-pellet and pellet-clad interfaces can significantly dictate the SNF system dynamic performance. With the consideration of interface bonding efficiency, the HBU SNF fuel property was estimated with CIRFT test data.

  2. Identification of nuclear protein targets for six leukemogenic tyrosine kinases governed by post-translational regulation.

    Directory of Open Access Journals (Sweden)

    Andrew Pierce

    Full Text Available Mutated tyrosine kinases are associated with a number of different haematological malignancies including myeloproliferative disorders, lymphoma and acute myeloid leukaemia. The potential commonalities in the action of six of these leukemogenic proteins on nuclear proteins were investigated using systematic proteomic analysis. The effects on over 3600 nuclear proteins and 1500 phosphopeptide sites were relatively quantified in seven isogenic cell lines. The effects of the kinases were diverse although some commonalities were found. Comparison of the nuclear proteomic data with transcriptome data and cytoplasmic proteomic data indicated that the major changes are due to post-translational mechanisms rather than changes in mRNA or protein distribution. Analysis of the promoter regions of genes whose protein levels changed in response to the kinases showed the most common binding site found was that for NFκB whilst other sites such as those for the glucocorticoid receptor were also found. Glucocorticoid receptor levels and phosphorylation were decreased by all 6 PTKs. Whilst Glucocorticoid receptor action can potentiate NFκB action those proteins where genes have NFκB binding sites were in often regulated post-translationally. However all 6 PTKs showed evidence of NFkB pathway modulation via activation via altered IkB and NFKB levels. Validation of a common change was also undertaken with PMS2, a DNA mismatch repair protein. PMS2 nuclear levels were decreased in response to the expression of all 6 kinases, with no concomitant change in mRNA level or cytosolic protein level. Response to thioguanine, that requires the mismatch repair pathway, was modulated by all 6 oncogenic kinases. In summary common targets for 6 oncogenic PTKs have been found that are regulated by post-translational mechanisms. They represent potential new avenues for therapies but also demonstrate the post-translational regulation is a key target of leukaemogenic kinases.

  3. History of the bubble chamber and related active- and internal-target nuclear tracking detectors

    International Nuclear Information System (INIS)

    Becchetti, F.D.

    2015-01-01

    Donald Glaser, 1960 Nobel laureate in Physics, recently passed away (2013), as have many of his colleagues who were involved with the early development of bubble chambers at the University of Michigan. In this paper I will review those early years and the subsequent wide-spread application of active-target (AT) bubble chambers that dominated high-energy physics (HEP) research for over thirty years. Some of the related, but more modern nuclear tracking detectors being used in HEP, neutrino astrophysics and dark-matter searches also will be discussed

  4. Efficient in planta gene targeting in tomato using geminiviral replicons and the CRISPR/Cas9 system.

    Science.gov (United States)

    Dahan-Meir, Tal; Filler-Hayut, Shdema; Melamed-Bessudo, Cathy; Bocobza, Samuel; Czosnek, Henryk; Aharoni, Asaph; Levy, Avraham A

    2018-04-18

    Current breeding relies mostly on random mutagenesis and recombination to generate novel genetic variation. However, targeted genome editing is becoming an increasingly important tool for precise plant breeding. Using the CRISPR-Cas system combined with the bean yellow dwarf virus rolling circle replicon we optimized a method for targeted mutagenesis and gene replacement in tomato. The carotenoid isomerase (CRTISO) and phytoene synthase 1 (PSY1) genes from the carotenoid biosynthesis pathway were chosen as targets due to their easily detectable change of phenotype. We took advantage of the geminiviral replicon amplification as a mean to provide a large amount of donor template for the repair of a CRISPR-Cas-induced DNA double strand break (DSB) in the target gene, via homologous recombination. Mutagenesis experiments, performed in the Micro-Tom variety achieved precise modification of the CRTISO and PSY1 loci at an efficiency of up to 90%. In the gene targeting experiments, our target was a fast-neutron-induced crtiso allele that contained a 281bp deletion. This deletion was repaired with the wildtype sequence through homologous recombination between the CRISPR-Cas-induced DSB in the crtiso target and the amplified donor in 25% of the plants transformed. This shows that efficient gene targeting can be achieved in the absence of selection markers or reporters using a single and modular construct that is adaptable to other tomato targets and other crops. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  5. Extended methods using thick-targets for nuclear reaction data of radioactive isotopes

    Science.gov (United States)

    Ebata, Shuichiro; Aikawa, Masayuki; Imai, Shotaro

    2017-09-01

    The nuclear transmutation is a technology to dispose of radioactive wastes. However, we do not have enough basic data for its developments, such as thick-target yields (TTY) and the interaction cross sections for radioactive material. We suggest two methods to estimate the TTY using inverse kinematics and to obtain the excitation function of the interaction cross sections which is named the thick-target transmission (T3) method. We deduce the energy-dependent conversion relation between the TTYs of the original system and its inverse kinematics, which can be replaced to a constant coefficient in the high energy region. Furthermore we show the usefulness of the T3 method to investigate the excitation function of the 12C + 27Al reaction in the simulation.

  6. Investigations of astrophysically interesting nuclear reactions by the use of gas target techniques

    Energy Technology Data Exchange (ETDEWEB)

    Hammer, J W [Inst. fuer Strahlenphysik, Univ. Stuttgart, Stuttgart (Germany)

    1998-06-01

    A brief review of the common properties of windowless and recirculating gas targets is presented. As example the Stuttgart gas target facility Rhinoceros in the extended and in the supersonic jet mode with its properties and techniques is explained, also with respect to gas purification techniques. Furthermore several typical experiments from the field of nuclear astrophysics with characteristic results are described (D({alpha},{gamma}){sup 6}Li, {sup 15}N({alpha},{gamma}){sup 19}F, {sup 16}O(p,{gamma}){sup 17}F, {sup 16}O({alpha},{gamma}){sup 20}Ne, {sup 20}Ne({alpha},{gamma}){sup 24}Mg, {sup 21}Ne({alpha},n){sup 24}Mg, {sup 18}O({alpha},n){sup 21}Ne, {sup 17}O({alpha},n){sup 20}Ne). In several cases the experimental sensitivity could be raised by up to a factor of 10{sup 6}. (orig.)

  7. Nuclear performance of target-blanket assemblies for electronuclear fuel production

    International Nuclear Information System (INIS)

    Alsmiller, R.G. Jr.; Gabriel, T.A.; Barish, J.

    1978-01-01

    The results of calculations of high energy transport carried out to evaluate the nuclear performance of several of the designs that have been proposed for electronuclear fuel production are presented. Topics covered include: results for 1-GeV protons and 1-GeV deuterons incident on a lithium target surrounded by a 238 U blanket; results for 1 GeV protons and 1-GeV deuterons incident on a thorium salt; results for 1-GeV protons, incident on a gas-cooled system fueled by either 238 UO 2 or 232 ThO 2 ; and results for protons in the energy range 0.5 to 10 GeV incident on a very large natural uranium target

  8. Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer.

    Science.gov (United States)

    Sung, Li-Ying; Gao, Shaorong; Shen, Hongmei; Yu, Hui; Song, Yifang; Smith, Sadie L; Chang, Ching-Chien; Inoue, Kimiko; Kuo, Lynn; Lian, Jin; Li, Ao; Tian, X Cindy; Tuck, David P; Weissman, Sherman M; Yang, Xiangzhong; Cheng, Tao

    2006-11-01

    Since the creation of Dolly via somatic cell nuclear transfer (SCNT), more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1%-5%) is that the clones are likely to be derived from adult stem cells. Support for this hypothesis comes from the findings that the reproductive cloning efficiency for embryonic stem cells is five to ten times higher than that for somatic cells as donors and that cloned pups cannot be produced directly from cloned embryos derived from differentiated B and T cells or neuronal cells. The question remains as to whether SCNT-derived animal clones can be derived from truly differentiated somatic cells. We tested this hypothesis with mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells, progenitor cells and granulocytes. We found that cloning efficiency increases over the differentiation hierarchy, and terminally differentiated postmitotic granulocytes yield cloned pups with the greatest cloning efficiency.

  9. The use of energy analysis and indexes of energy efficiency in nuclear power

    International Nuclear Information System (INIS)

    D'yakonov, E.I.; Ignatenko, E.I.

    1991-01-01

    The results of calculating the indexes of energy efficiency for NPPs with the WWER-1000 and RBMK-1000 reactors, heat and power NPPs with the WWER-1000 and dictrict heating NPPs with the AST-500 reactor in three fuel cycles, namely, the open one and with uranium and plutonium recycles, are considered. Complex account for the quantity and quality of produced and consumed energy provides for objective evaluation of the indexes of energy efficiency during comparative analysis of nuclear power plants with different types of reactors. It is shown that complex use of the energy produced at a NPP provides for increase of indexes of energy efficiency. The highest indexes are obtained for heat and power NPP with the WWER-1000 reactor in the open fuel cycle, with uranium and plutonium recycle and for NPP with the WWER-1000 reactor with plutonium recycle

  10. Myocardial regeneration in adriamycin cardiomyopathy by nuclear expression of GLP1 using ultrasound targeted microbubble destruction

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Shuyuan [Baylor Research Institute, Baylor University Medical Center, 3812 Elm Street, Dallas, TX (United States); Chen, Jiaxi [The University of Texas Southwestern Medical Center at Dallas, Medical School, 5235 Harry Hine Blvd., Dallas, TX (United States); Huang, Pintong [Department of Ultrasonography, The 2nd Affiliated Hospital of Zhejiang University College of Medicine, Hangzhou, Zhejiang Province (China); Meng, Xing-Li; Clayton, Sandra; Shen, Jin-Song [Baylor Research Institute, Baylor University Medical Center, 3812 Elm Street, Dallas, TX (United States); Grayburn, Paul A., E-mail: paulgr@baylorhealth.edu [Baylor Research Institute, Baylor University Medical Center, 3812 Elm Street, Dallas, TX (United States); Department of Internal Medicine, Division of Cardiology, Baylor Heart and Vascular Institute, Baylor University Medical Center, 621 N. Hall St, Suite H030, Dallas, TX (United States)

    2015-03-20

    Recently GLP-1 was found to have cardioprotective effects independent of those attributable to tight glycemic control. Methods and results: We employed ultrasound targeted microbubble destruction (UTMD) to deliver piggybac transposon plasmids encoding the GLP-1 gene with a nuclear localizing signal to rat hearts with adriamycin cardiomyopathy. After a single UTMD treatment, overexpression of transgenic GLP-1 was found in nuclei of rat heart cells with evidence that transfected cardiac cells had undergone proliferation. UTMD-GLP-1 gene therapy restored LV mass, fractional shortening index, and LV posterior wall diameter to nearly normal. Nuclear overexpression of GLP-1 by inducing phosphorylation of FoxO1-S256 and translocation of FoxO1 from the nucleus to the cytoplasm significantly inactivated FoxO1 and activated the expression of cyclin D1 in nuclei of cardiac muscle cells. Reversal of adriamycin cardiomyopathy appeared to be mediated by dedifferentiation and proliferation of nuclear FoxO1-positive cardiac muscle cells with evidence of embryonic stem cell markers (OCT4, Nanog, SOX2 and c-kit), cardiac early differentiation markers (NKX2.5 and ISL-1) and cellular proliferation markers (BrdU and PHH3) after UTMD with GLP-1 gene therapy. Conclusions: Intranuclear myocardial delivery of the GLP-1gene can reverse established adriamycin cardiomyopathy by stimulating myocardial regeneration. - Highlights: • The activation of nuclear FoxO1 in cardiac muscle cells associated with adriamycin cardiomyopathy. • Myocardial nuclear GLP-1 stimulates myocardial regeneration and reverses adriamycin cardiomyopathy. • The process of myocardial regeneration associated with dedifferentiation and proliferation.

  11. Myocardial regeneration in adriamycin cardiomyopathy by nuclear expression of GLP1 using ultrasound targeted microbubble destruction

    International Nuclear Information System (INIS)

    Chen, Shuyuan; Chen, Jiaxi; Huang, Pintong; Meng, Xing-Li; Clayton, Sandra; Shen, Jin-Song; Grayburn, Paul A.

    2015-01-01

    Recently GLP-1 was found to have cardioprotective effects independent of those attributable to tight glycemic control. Methods and results: We employed ultrasound targeted microbubble destruction (UTMD) to deliver piggybac transposon plasmids encoding the GLP-1 gene with a nuclear localizing signal to rat hearts with adriamycin cardiomyopathy. After a single UTMD treatment, overexpression of transgenic GLP-1 was found in nuclei of rat heart cells with evidence that transfected cardiac cells had undergone proliferation. UTMD-GLP-1 gene therapy restored LV mass, fractional shortening index, and LV posterior wall diameter to nearly normal. Nuclear overexpression of GLP-1 by inducing phosphorylation of FoxO1-S256 and translocation of FoxO1 from the nucleus to the cytoplasm significantly inactivated FoxO1 and activated the expression of cyclin D1 in nuclei of cardiac muscle cells. Reversal of adriamycin cardiomyopathy appeared to be mediated by dedifferentiation and proliferation of nuclear FoxO1-positive cardiac muscle cells with evidence of embryonic stem cell markers (OCT4, Nanog, SOX2 and c-kit), cardiac early differentiation markers (NKX2.5 and ISL-1) and cellular proliferation markers (BrdU and PHH3) after UTMD with GLP-1 gene therapy. Conclusions: Intranuclear myocardial delivery of the GLP-1gene can reverse established adriamycin cardiomyopathy by stimulating myocardial regeneration. - Highlights: • The activation of nuclear FoxO1 in cardiac muscle cells associated with adriamycin cardiomyopathy. • Myocardial nuclear GLP-1 stimulates myocardial regeneration and reverses adriamycin cardiomyopathy. • The process of myocardial regeneration associated with dedifferentiation and proliferation

  12. High-efficiency targeted editing of large viral genomes by RNA-guided nucleases.

    Science.gov (United States)

    Bi, Yanwei; Sun, Le; Gao, Dandan; Ding, Chen; Li, Zhihua; Li, Yadong; Cun, Wei; Li, Qihan

    2014-05-01

    A facile and efficient method for the precise editing of large viral genomes is required for the selection of attenuated vaccine strains and the construction of gene therapy vectors. The type II prokaryotic CRISPR-Cas (clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas)) RNA-guided nuclease system can be introduced into host cells during viral replication. The CRISPR-Cas9 system robustly stimulates targeted double-stranded breaks in the genomes of DNA viruses, where the non-homologous end joining (NHEJ) and homology-directed repair (HDR) pathways can be exploited to introduce site-specific indels or insert heterologous genes with high frequency. Furthermore, CRISPR-Cas9 can specifically inhibit the replication of the original virus, thereby significantly increasing the abundance of the recombinant virus among progeny virus. As a result, purified recombinant virus can be obtained with only a single round of selection. In this study, we used recombinant adenovirus and type I herpes simplex virus as examples to demonstrate that the CRISPR-Cas9 system is a valuable tool for editing the genomes of large DNA viruses.

  13. High-efficiency targeted editing of large viral genomes by RNA-guided nucleases.

    Directory of Open Access Journals (Sweden)

    Yanwei Bi

    2014-05-01

    Full Text Available A facile and efficient method for the precise editing of large viral genomes is required for the selection of attenuated vaccine strains and the construction of gene therapy vectors. The type II prokaryotic CRISPR-Cas (clustered regularly interspaced short palindromic repeats (CRISPR-associated (Cas RNA-guided nuclease system can be introduced into host cells during viral replication. The CRISPR-Cas9 system robustly stimulates targeted double-stranded breaks in the genomes of DNA viruses, where the non-homologous end joining (NHEJ and homology-directed repair (HDR pathways can be exploited to introduce site-specific indels or insert heterologous genes with high frequency. Furthermore, CRISPR-Cas9 can specifically inhibit the replication of the original virus, thereby significantly increasing the abundance of the recombinant virus among progeny virus. As a result, purified recombinant virus can be obtained with only a single round of selection. In this study, we used recombinant adenovirus and type I herpes simplex virus as examples to demonstrate that the CRISPR-Cas9 system is a valuable tool for editing the genomes of large DNA viruses.

  14. A Viral Receptor Complementation Strategy to Overcome CAV-2 Tropism for Efficient Retrograde Targeting of Neurons.

    Science.gov (United States)

    Li, Shu-Jing; Vaughan, Alexander; Sturgill, James Fitzhugh; Kepecs, Adam

    2018-06-06

    Retrogradely transported neurotropic viruses enable genetic access to neurons based on their long-range projections and have become indispensable tools for linking neural connectivity with function. A major limitation of viral techniques is that they rely on cell-type-specific molecules for uptake and transport. Consequently, viruses fail to infect variable subsets of neurons depending on the complement of surface receptors expressed (viral tropism). We report a receptor complementation strategy to overcome this by potentiating neurons for the infection of the virus of interest-in this case, canine adenovirus type-2 (CAV-2). We designed AAV vectors for expressing the coxsackievirus and adenovirus receptor (CAR) throughout candidate projection neurons. CAR expression greatly increased retrograde-labeling rates, which we demonstrate for several long-range projections, including some resistant to other retrograde-labeling techniques. Our results demonstrate a receptor complementation strategy to abrogate endogenous viral tropism and thereby facilitate efficient retrograde targeting for functional analysis of neural circuits. Copyright © 2018 Elsevier Inc. All rights reserved.

  15. Improvement of uranium production efficiency to meet China's nuclear power requirements

    International Nuclear Information System (INIS)

    Zhang, R.

    1997-01-01

    Recently China put the Qinshan Nuclear Power Plant, with an installed capacity of 300 MW, in the province of Zhejiang and the Daya Bay Nuclear Power Plant, with a total installed capacity of 2 x 900 MW, in commercial operation. China plans a rapid growth in nuclear power from 1995 to 2010. China's uranium production will therefore also enter a new period with nuclear power increasing. In order to meet the demand of nuclear power for uranium special attention has been paid to both technical progress improvement using management with the aim of reducing the cost of uranium production. The application of the trackless mining technique has enhanced the uranium mining productivity significantly. China has produced a radiometric sorter, model 5421-2 for pre-concentrating uranium run-of-mine ore. This effectively increases the uranium content in mill feed and decreases the operating cost of hydrometallurgical treatment. The in situ leach technique after blasting is applied underground in the Lantian Mine, in addition to the surface heap leaching, and has obtained a perfect result. The concentrated acid-curing, and ferric sulphate trickle leaching process, will soon be used in commercial operation for treating uranium ore grading -5 to -7 mm in size. The annual production capability of the Yining Mine will be extended to 100 tonnes U using improving in situ leaching technology. For the purpose of improving the uranium production efficiency much work has been done optimizing the distribution of production centres. China plans to expand its uranium production to meet the uranium requirements of the developing nuclear power plants. (author). 4 tabs

  16. Non-classical nuclear localization signal peptides for high efficiency lipofection of primary neurons and neuronal cell lines.

    Science.gov (United States)

    Ma, H; Zhu, J; Maronski, M; Kotzbauer, P T; Lee, V M-Y; Dichter, M A; Diamond, S L

    2002-01-01

    Gene transfer into CNS is critical for potential therapeutic applications as well as for the study of the genetic basis of neural development and nerve function. Unfortunately, lipid-based gene transfer to CNS cells is extremely inefficient since the nucleus of these post-mitotic cells presents a significant barrier to transfection. We report the development of a simple and highly efficient lipofection method for primary embryonic rat hippocampal neurons (up to 25% transfection) that exploits the M9 sequence of the non-classical nuclear localization signal of heterogeneous nuclear ribonucleoprotein A1 for targeting beta(2)-karyopherin (transportin-1). M9-assistant lipofection resulted in 20-100-fold enhancement of transfection over lipofection alone for embryonic-derived retinal ganglion cells, rat pheochromocytoma (PC12) cells, embryonic rat ventral mesencephalon neurons, as well as the clinically relevant human NT2 cells or retinoic acid-differentiated NT2 neurons. This technique can facilitate the implementation of promoter construct experiments in post-mitotic cells, stable transformant generation, and dominant-negative mutant expression techniques in CNS cells.

  17. Nuclear targeting by fragmentation of the Potato spindle tuber viroid genome

    International Nuclear Information System (INIS)

    Abraitiene, Asta; Zhao Yan; Hammond, Rosemarie

    2008-01-01

    Transient expression of engineered reporter RNAs encoding an intron-containing green fluorescent protein (GFP) from a Potato virus X-based expression vector previously demonstrated the nuclear targeting capability of the 359 nucleotide Potato spindle tuber viroid (PSTVd) RNA genome. To further delimit the putative nuclear-targeting signal, PSTVd subgenomic fragments were embedded within the intron, and recombinant reporter RNAs were inoculated onto Nicotiana benthamiana plants. Appearance of green fluorescence in leaf tissue inoculated with PSTVd-fragment-containing constructs indicated shuttling of the RNA into the nucleus by fragments as short as 80 nucleotides in length. Plant-to-plant variation in the timing of intron removal and subsequent GFP fluorescence was observed; however, earliest and most abundant GFP expression was obtained with constructs containing the conserved hairpin I palindrome structure and embedded upper central conserved region. Our results suggest that this conserved sequence and/or the stem-loop structure it forms is sufficient for import of PSTVd into the nucleus

  18. Evolutionary conservation of nuclear and nucleolar targeting sequences in yeast ribosomal protein S6A

    International Nuclear Information System (INIS)

    Lipsius, Edgar; Walter, Korden; Leicher, Torsten; Phlippen, Wolfgang; Bisotti, Marc-Angelo; Kruppa, Joachim

    2005-01-01

    Over 1 billion years ago, the animal kingdom diverged from the fungi. Nevertheless, a high sequence homology of 62% exists between human ribosomal protein S6 and S6A of Saccharomyces cerevisiae. To investigate whether this similarity in primary structure is mirrored in corresponding functional protein domains, the nuclear and nucleolar targeting signals were delineated in yeast S6A and compared to the known human S6 signals. The complete sequence of S6A and cDNA fragments was fused to the 5'-end of the LacZ gene, the constructs were transiently expressed in COS cells, and the subcellular localization of the fusion proteins was detected by indirect immunofluorescence. One bipartite and two monopartite nuclear localization signals as well as two nucleolar binding domains were identified in yeast S6A, which are located at homologous regions in human S6 protein. Remarkably, the number, nature, and position of these targeting signals have been conserved, albeit their amino acid sequences have presumably undergone a process of co-evolution with their corresponding rRNAs

  19. Nucleolin is a nuclear target of heparan sulfate derived from glypican-1

    International Nuclear Information System (INIS)

    Cheng, Fang; Belting, Mattias; Fransson, Lars-Åke; Mani, Katrin

    2017-01-01

    The recycling, S-nitrosylated heparan sulfate (HS) proteoglycan glypican-1 releases anhydromannose (anMan)-containing HS chains by a nitrosothiol-catalyzed cleavage in endosomes that can be constitutive or induced by ascorbate. The HS-anMan chains are then transported to the nucleus. A specific nuclear target for HS-anMan has not been identified. We have monitored endosome-to-nucleus trafficking of HS-anMan by deconvolution and confocal immunofluorescence microscopy using an anMan-specific monoclonal antibody in non-growing, ascorbate-treated, and growing, untreated, wild-type mouse embryonic fibroblasts and hypoxia-exposed Alzheimer mouse Tg2576 fibroblasts and human U87 glioblastoma cells. In all cells, nuclear HS-anMan targeted a limited number of sites of variable size where it colocalized with DNA and nucleolin, an established marker for nucleoli. HS-anMan also colocalized with ethynyl uridine-tagged nascent RNA and two acetylated forms of histone H3. Acute hypoxia increased the formation of HS-anMan in both Tg2576 and U87 cells. A portion of HS-anMan colocalized with nucleolin at small discrete sites, while most of the nucleolin and nascent RNA was dispersed. In U87 cells, HS-anMan, nucleolin and nascent RNA reassembled after prolonged hypoxia. Nucleolar HS may modulate synthesis and/or release of rRNA.

  20. Targets and criteria for the effective participation of national industry in a domestic nuclear power programme

    International Nuclear Information System (INIS)

    Py, J.P.

    1986-01-01

    The interest in maximum use of national resource is common to all countries, the highly industrialized as well as the developing ones. Although benefits can be expected from national participation in a domestic nuclear power programme and may not be limited to this programme, such national participation is restricted by constraining factors: economic, financial, technical and political. Considering the various natures of activities - design, procurement, manufacturing, erection -, their technical difficulties, their potential spin-off effects on the overall industrial development of a country, the paper reviews the materials and components of a nuclear power plants which can be selected as targets for domestic production. The paper also reviews criteria which must be considered in setting these Target materials and components in order to overcome restricting factors to national participation such as cost of national products, financing, investment capability, adequate market size, availability of qualified manpower, industrial capability and quality standards, availability of technology and know-how, conflicts of interests. Some concrete examples drawn from previous experience will illustrate France efforts to overcome these limiting factors [fr

  1. Nucleolin is a nuclear target of heparan sulfate derived from glypican-1

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, Fang [Department of Experimental Medical Science, Division of Neuroscience, Glycobiology Group, Lund University, Biomedical Center A13, SE-221 84 Lund (Sweden); Belting, Mattias [Department of Clinical Sciences, Section of Oncology and Pathology, Lund University, Lund (Sweden); Fransson, Lars-Åke [Department of Experimental Medical Science, Division of Neuroscience, Glycobiology Group, Lund University, Biomedical Center A13, SE-221 84 Lund (Sweden); Mani, Katrin, E-mail: katrin.mani@med.lu.se [Department of Experimental Medical Science, Division of Neuroscience, Glycobiology Group, Lund University, Biomedical Center A13, SE-221 84 Lund (Sweden)

    2017-05-01

    The recycling, S-nitrosylated heparan sulfate (HS) proteoglycan glypican-1 releases anhydromannose (anMan)-containing HS chains by a nitrosothiol-catalyzed cleavage in endosomes that can be constitutive or induced by ascorbate. The HS-anMan chains are then transported to the nucleus. A specific nuclear target for HS-anMan has not been identified. We have monitored endosome-to-nucleus trafficking of HS-anMan by deconvolution and confocal immunofluorescence microscopy using an anMan-specific monoclonal antibody in non-growing, ascorbate-treated, and growing, untreated, wild-type mouse embryonic fibroblasts and hypoxia-exposed Alzheimer mouse Tg2576 fibroblasts and human U87 glioblastoma cells. In all cells, nuclear HS-anMan targeted a limited number of sites of variable size where it colocalized with DNA and nucleolin, an established marker for nucleoli. HS-anMan also colocalized with ethynyl uridine-tagged nascent RNA and two acetylated forms of histone H3. Acute hypoxia increased the formation of HS-anMan in both Tg2576 and U87 cells. A portion of HS-anMan colocalized with nucleolin at small discrete sites, while most of the nucleolin and nascent RNA was dispersed. In U87 cells, HS-anMan, nucleolin and nascent RNA reassembled after prolonged hypoxia. Nucleolar HS may modulate synthesis and/or release of rRNA.

  2. International Target Values 2010 for Measurement Uncertainties in Safeguarding Nuclear Materials

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, M.; Penkin, M.; Norman, C.; Balsley, S. [IAEA, Vienna (Australia); others, and

    2012-12-15

    This issue of the International Target Values (ITVs) represents the sixth revision, following the first release of such tables issued in 1979 by the ESARDA/WGDA. The ITVs are uncertainties to be considered in judging the reliability of analytical techniques applied to industrial nuclear and fissile material, which are subject to safeguards verification. The tabulated values represent estimates of the 'state of the practice' which should be achievable under routine measurement conditions. The most recent standard conventions in representing uncertainty have been considered, while maintaining a format that allows comparison with the previous releases of the ITVs. The present report explains why target values are needed, how the concept evolved and how they relate to the operator's and inspector's measurement systems. The ITVs-2010 are intended to be used by plant operators and safeguards organizations, as a reference of the quality of measurements achievable in nuclear material accountancy, and for planning purposes. The report suggests that the use of ITVs can be beneficial for statistical inferences regarding the significance of operator-inspector differences whenever valid performance values are not available.

  3. International target values 2010 for achievable measurement uncertainties in nuclear material accountancy

    Energy Technology Data Exchange (ETDEWEB)

    Dias, Fabio C., E-mail: fabio@ird.gov.b [Comissao Nacional de Energia Nuclear (CNEN), Rio de Janeiro, RJ (Brazil); Almeida, Silvio G. de; Renha Junior, Geraldo, E-mail: silvio@abacc.org.b, E-mail: grenha@abacc.org.b [Agencia Brasileiro-Argentina de Contabilidade e Controle de Materiais Nucleares (ABACC), Rio de Janeiro, RJ (Brazil)

    2011-07-01

    The International Target Values (ITVs) are reasonable uncertainty estimates that can be used in judging the reliability of measurement techniques applied to industrial nuclear and fissile materials subject to accountancy and/or safeguards verification. In the absence of relevant experimental estimates, ITVs can also be used to select measurement techniques and calculate sample population during the planning phase of verification activities. It is important to note that ITVs represent estimates of the 'state-of-the-practice', which should be achievable under routine measurement conditions affecting both facility operators and safeguards inspectors, not only in the field, but also in laboratory. Tabulated values cover measurement methods used for the determination of volume or mass of the nuclear material, for its elemental and isotopic assays, and for its sampling. The 2010 edition represents the sixth revision of the International Target Values (ITVs), issued by the International Atomic Energy Agency (IAEA) as a Safeguards Technical Report (STR-368). The first version was released as 'Target Values' in 1979 by the Working Group on Techniques and Standards for Destructive Analysis (WGDA) of the European Safeguards Research and Development Association (ESARDA) and focused on destructive analytical methods. In the latest 2010 revision, international standards in estimating and expressing uncertainties have been considered while maintaining a format that allows comparison with the previous editions of the ITVs. Those standards have been usually applied in QC/QA programmes, as well as qualification of methods, techniques and instruments. Representatives of the Brazilian Nuclear Energy Commission (CNEN) and the Brazilian-Argentine Agency for Accounting and Control of Nuclear Materials (ABACC) participated in previous Consultants Group Meetings since the one convened to establish the first list of ITVs released in 1993 and in subsequent revisions

  4. A study on intensifying efficiency for international collaborative development of advanced nuclear energy technology

    Energy Technology Data Exchange (ETDEWEB)

    Lee, J. H.; Hahn, D. H.; Song, K. C.; Chang, J. H.; Kim, H. J.; Kim, H. J.; Lim, C. Y.; Lee, D. S.; Lee, Y. J. [KAERI, Daejeon (Korea, Republic of)

    2011-03-15

    The objective of the study was to participate the GIF for the efficient propulsion of future nuclear system development. For achieving the objective of this study, the followings were carried out. {Omicron} Analyze the international/domestic trends in the future nuclear energy system {Omicron} Analyze the domestic long-term R and D program for the future nuclear system and assist its implementation - Review the agenda of the executive committee, the technical committee, and sub-technical committee - Assist the committee meetings and workshops related to the future nuclear energy system {Omicron} Develop the participation strategy for the collaborative development of Gen-IV technology and conducting the international cooperation activities - Support the delegation by reviewing the agenda of GIF meetings in the technical and legal perspective - Research the system R and D arrangement and report its progress - Participate in the SFR SIA PA negotiation meeting and report its progress {Omicron} Support the activities related to I-NERI between Korea and U.S. - Support a delegation by reviewing the agenda in the technical/legal point of view - Participate in the BINERIC meetings and Support the related activities The result of this study may be used for 1) contribution to establishing the effective foundation and broadening the cooperation activities between the advanced countries and Korea and 2) contribution effective management of Gen IV international collaboration by technical/legal supporting

  5. A Study on intensifying efficiency for international collaborative development of Advanced Nuclear Energy Technology

    International Nuclear Information System (INIS)

    Chang, Moon Hee; Kim, H. R.; Kim, H. J.

    2009-08-01

    The objective of the study was to participate the GIF for the efficient propulsion of future nuclear system development. For achieving the objective of this study, the followings were carried out. · Investigation and analysis of the international and domestic trends related to future nuclear system · To maximize the national interests by the strategic participation of GIF meeting - To participate of GIF meeting and to support of relative work - To investigate the System R and D Arrangement and to inform its progress situation · To maximize the propulsion results of Korea/U.S nuclear energy joint research(I-NERI) - To support a delegation by the review of agenda in aspect of the technical/legal point - To participate of BINERIC meeting and to support of relative work · Streamline the nuclear energy R and D due to the effective connection between domestic R and D and international collaboration The result of this study may be used for 1) contribution to establishing the effective foundation and broadening the cooperation activities between the advanced countries and Korea and 2) contribution effective management of Gen IV international collaboration by technical/legal supporting

  6. Diagnosis of Thermal Efficiency of Nuclear Power Plants Using Optical Torque Sensors

    International Nuclear Information System (INIS)

    Shuichi Umezawa; Jun Adachi

    2006-01-01

    A new optical torque measuring method was applied to diagnosis of thermal efficiency of nuclear power plants. The sensor allows torque deformation of the rotor caused by power transmission to be measured without contact. Semiconductor laser beams and small pieces of stainless reflector that have bar-code patterns are employed. The intensity of the reflected laser beam is measured and then input into a computer through an APD and an A/D converter having high frequency sampling rates. The correlation analysis technique can translate these data into the torque deformation angle. This angle allows us to obtain the turbine output along with the torsional rigidity and the rotating speed of the rotor. The sensor was applied to a nuclear plant of Tokyo Electric Power Company, TEPCO, following its application success to the early combined cycle plants and the advanced combined cycle plants of TEPCO. As the turbine rotor of the nuclear power plant is less exposed than that of the combined cycle plants, the measurement position is confined to a narrow gap. In order to overcome the difficulty in installation, the shape of the sensor is modified to be long and thin. Sensor performance of the nuclear power plant was inspected over a year. The value of the torsional rigidity was analyzed by the finite element method at first. Accuracy was improved by correcting the torsional rigidity so that the value was consistent with the generator output. As a result, it is considered that the sensor performance has reached a practical use level. (authors)

  7. A study on intensifying efficiency for international collaborative development of advanced nuclear energy technology

    International Nuclear Information System (INIS)

    Lee, J. H.; Hahn, D. H.; Song, K. C.; Chang, J. H.; Kim, H. J.; Kim, H. J.; Lim, C. Y.; Lee, D. S.; Lee, Y. J.

    2011-03-01

    The objective of the study was to participate the GIF for the efficient propulsion of future nuclear system development. For achieving the objective of this study, the followings were carried out. Ο Analyze the international/domestic trends in the future nuclear energy system Ο Analyze the domestic long-term R and D program for the future nuclear system and assist its implementation - Review the agenda of the executive committee, the technical committee, and sub-technical committee - Assist the committee meetings and workshops related to the future nuclear energy system Ο Develop the participation strategy for the collaborative development of Gen-IV technology and conducting the international cooperation activities - Support the delegation by reviewing the agenda of GIF meetings in the technical and legal perspective - Research the system R and D arrangement and report its progress - Participate in the SFR SIA PA negotiation meeting and report its progress Ο Support the activities related to I-NERI between Korea and U.S. - Support a delegation by reviewing the agenda in the technical/legal point of view - Participate in the BINERIC meetings and Support the related activities The result of this study may be used for 1) contribution to establishing the effective foundation and broadening the cooperation activities between the advanced countries and Korea and 2) contribution effective management of Gen IV international collaboration by technical/legal supporting

  8. High efficiency of targeted mutagenesis in arabidopsis via meiotic promoter-driven expression of Cas9 endonuclease

    KAUST Repository

    Eid, Ayman

    2016-05-28

    Key message: The use of a meiosis I-specific promoter increased the efficiency of targeted mutagenesis and will facilitate the manipulation of homologous recombination. Abstract: The CRISPR/Cas9 system has been harnessed for targeted engineering of eukaryotic genomes, including plants; however, CRISPR/Cas9 efficiency varies considerably in different plant tissues and species. In Arabidopsis, the generation of homozygous or bi-allelic mutants in the first (T1) generation is inefficient. Here, we used specific promoters to drive the expression of Cas9 during meiosis to maximize the efficiency of recovering heritable mutants in T1 plants. Our data reveal that the use of a promoter active in meiosis I resulted in high-efficiency (28 %) recovery of targeted mutants in the T1 generation. Moreover, this method enabled efficient simultaneous targeting of three genes for mutagenesis. Taken together, our results show that the use of meiosis-specific promoters will improve methods for functional genomic analysis and studying the molecular underpinnings of homologous recombination. © 2016, Springer-Verlag Berlin Heidelberg.

  9. Histone deacetylase inhibitor significantly improved the cloning efficiency of porcine somatic cell nuclear transfer embryos.

    Science.gov (United States)

    Huang, Yongye; Tang, Xiaochun; Xie, Wanhua; Zhou, Yan; Li, Dong; Yao, Chaogang; Zhou, Yang; Zhu, Jianguo; Lai, Liangxue; Ouyang, Hongsheng; Pang, Daxin

    2011-12-01

    Valproic acid (VPA), a histone deacetylase inbibitor, has been shown to generate inducible pluripotent stem (iPS) cells from mouse and human fibroblasts with a significant higher efficiency. Because successful cloning by somatic cell nuclear transfer (SCNT) undergoes a full reprogramming process in which the epigenetic state of a differentiated donor nuclear is converted into an embryonic totipotent state, we speculated that VPA would be useful in promoting cloning efficiency. Therefore, in the present study, we examined whether VPA can promote the developmental competence of SCNT embryos by improving the reprogramming state of donor nucleus. Here we report that 1 mM VPA for 14 to 16 h following activation significantly increased the rate of blastocyst formation of porcine SCNT embryos constructed from Landrace fetal fibroblast cells compared to the control (31.8 vs. 11.4%). However, we found that the acetylation level of Histone H3 lysine 14 and Histone H4 lysine 5 and expression level of Oct4, Sox2, and Klf4 was not significantly changed between VPA-treated and -untreated groups at the blastocyst stage. The SCNT embryos were transferred to 38 surrogates, and the cloning efficiency in the treated group was significantly improved compared with the control group. Taken together, we have demonstrated that VPA can improve both in vitro and in vivo development competence of porcine SCNT embryos.

  10. Efficiency of nuclear and mitochondrial markers recovering and supporting known amniote groups.

    Science.gov (United States)

    Lambret-Frotté, Julia; Perini, Fernando Araújo; de Moraes Russo, Claudia Augusta

    2012-01-01

    We have analysed the efficiency of all mitochondrial protein coding genes and six nuclear markers (Adora3, Adrb2, Bdnf, Irbp, Rag2 and Vwf) in reconstructing and statistically supporting known amniote groups (murines, rodents, primates, eutherians, metatherians, therians). The efficiencies of maximum likelihood, Bayesian inference, maximum parsimony, neighbor-joining and UPGMA were also evaluated, by assessing the number of correct and incorrect recovered groupings. In addition, we have compared support values using the conservative bootstrap test and the Bayesian posterior probabilities. First, no correlation was observed between gene size and marker efficiency in recovering or supporting correct nodes. As expected, tree-building methods performed similarly, even UPGMA that, in some cases, outperformed other most extensively used methods. Bayesian posterior probabilities tend to show much higher support values than the conservative bootstrap test, for correct and incorrect nodes. Our results also suggest that nuclear markers do not necessarily show a better performance than mitochondrial genes. The so-called dependency among mitochondrial markers was not observed comparing genome performances. Finally, the amniote groups with lowest recovery rates were therians and rodents, despite the morphological support for their monophyletic status. We suggest that, regardless of the tree-building method, a few carefully selected genes are able to unfold a detailed and robust scenario of phylogenetic hypotheses, particularly if taxon sampling is increased.

  11. On economic efficiency of nuclear power unit life extension using steam-gas topping plant

    International Nuclear Information System (INIS)

    Kuznetsov, Y.N.; Lisitsa, F.D.; Smirnov, V.G.

    2001-01-01

    The different options for life extension of the operating nuclear power units have been analyzed in the report with regard for their economic efficiency. A particular attention is given to the option envisaging the reduction of reactor power output and its subsequent compensation with a steam-gas topping plant. Steam generated at its heat-recovery boilers is proposed to be used for the additional loading of the nuclear plant turbine so as to reach its nominal output. It would be demonstrated that the implementation of this option allows to reduce total costs in the period of power plant life extension by 24-29% as compared with the alternative use of the replacing steam-gas unit and the saved resources could be directed, for instance, for decommissioning of a reactor facility. (authors)

  12. Phase I aging assessment of nuclear air-treatment system high efficiency particulate air and adsorbers

    International Nuclear Information System (INIS)

    Winegardner, W.K.

    1996-01-01

    A phase I aging assessment of high efficiency particulate air filters and activated carbon gas adsorption units was performed by the Pacific Northwest Laboratory as part of the US Nuclear Regulatory Commission's Nuclear Plant Aging Research Program. Information was compiled concerning design features, failure experience, aging mechanisms, effects, and stressors, and monitoring methods. Over 1100 failures, or 12% of the filter installations, were reported as part of a US Department of energy survey. Investigators from other laboratories have suggested that aging could have contributed to over 80% of these failures. Several instances of impaired performance as the result of the premature aging of carbon were reported. Filter aging mechanisms range from those associated with particle loading to reactions that alter the properties of gaskets. Mechanisms that can lead to impaired adsorber performance include the loss of potentially available active sites as a result of the adsorption of moisture or pollutants. Stressors include heat, moisture, radiation, and airborne particles and contaminants. (orig.)

  13. Advanced control systems to improve nuclear power plant reliability and efficiency

    International Nuclear Information System (INIS)

    1997-07-01

    The TECDOC is the result of a series of an advisory and consultants meetings held by the IAEA in 1995-1996 in Vienna (March 1995), in Erlangen Germany (December 1995), in Garching, Germany (June 1996) and in Vienna (November 1996). It was prepared with the participation and contributions of experts from Austria, Canada, Finland, France, Germany, the Republic of Korea, Norway, the Russian Federation, the United Kingdom and the United States of America. The publication not only describes advanced control systems for the improvement of nuclear power plant reliability and efficiency, but also provides a road map to guide interested readers to plan and execute an advanced instrumentation and control project. The subjects include: identification of needs and requirements, justification for safety and user acceptance, and the development of an engineering process. The report should be of interest to nuclear power plant staff, I and C system designers and integrators as well as regulators and researchers. Refs, figs, tabs

  14. Advanced control systems to improve nuclear power plant reliability and efficiency

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    The TECDOC is the result of a series of an advisory and consultants meetings held by the IAEA in 1995-1996 in Vienna (March 1995), in Erlangen Germany (December 1995), in Garching, Germany (June 1996) and in Vienna (November 1996). It was prepared with the participation and contributions of experts from Austria, Canada, Finland, France, Germany, the Republic of Korea, Norway, the Russian Federation, the United Kingdom and the United States of America. The publication not only describes advanced control systems for the improvement of nuclear power plant reliability and efficiency, but also provides a road map to guide interested readers to plan and execute an advanced instrumentation and control project. The subjects include: identification of needs and requirements, justification for safety and user acceptance, and the development of an engineering process. The report should be of interest to nuclear power plant staff, I and C system designers and integrators as well as regulators and researchers. Refs, figs, tabs.

  15. Prospects for and problems of using light-water supercritical-pressure coolant in nuclear reactors in order to increase the efficiency of the nuclear fuel cycle

    International Nuclear Information System (INIS)

    Alekseev, P. N.; Semchenkov, Yu. M.; Sedov, A. A.; Subbotin, S. A.; Chibinyaev, A. V.

    2011-01-01

    Trends in the development of the power sector of the Russian and world power industries both at present time and in the near future are analyzed. Trends in the rise of prices for reserves of fossil and nuclear fuels used for electricity production are compared. An analysis of the competitiveness of electricity production at nuclear power plants as compared to the competitiveness of electricity produced at coal-fired and natural-gas-fired thermal power plants is performed. The efficiency of the open nuclear fuel cycle and various versions of the closed nuclear fuel cycle is discussed. The requirements on light-water reactors under the scenario of dynamic development of the nuclear power industry in Russia are determined. Results of analyzing the efficiency of fuel utilization for various versions of vessel-type light-water reactors with supercritical coolant are given. Advantages and problems of reactors with supercritical-pressure water are listed.

  16. Ratio between autoflocculating and target microalgae affects the energy-efficient harvesting by bio-flocculation

    NARCIS (Netherlands)

    Salim, S.; Vermuë, M.H.; Wijffels, R.H.

    2012-01-01

    The effect of ratio between autoflocculating and target microalgae in bio-flocculation was studied with emphasis on the recovery, sedimentation rate and energy demand for harvesting the target microalgae. When the autoflocculating microalgae Ettlia texensis, Ankistrodesmus falcatus and Scenedesmus

  17. Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm.

    Science.gov (United States)

    Zhou, Hong; Zhou, Michael; Li, Daisy; Manthey, Joseph; Lioutikova, Ekaterina; Wang, Hong; Zeng, Xiao

    2017-11-17

    The beauty and power of the genome editing mechanism, CRISPR Cas9 endonuclease system, lies in the fact that it is RNA-programmable such that Cas9 can be guided to any genomic loci complementary to a 20-nt RNA, single guide RNA (sgRNA), to cleave double stranded DNA, allowing the introduction of wanted mutations. Unfortunately, it has been reported repeatedly that the sgRNA can also guide Cas9 to off-target sites where the DNA sequence is homologous to sgRNA. Using human genome and Streptococcus pyogenes Cas9 (SpCas9) as an example, this article mathematically analyzed the probabilities of off-target homologies of sgRNAs and discovered that for large genome size such as human genome, potential off-target homologies are inevitable for sgRNA selection. A highly efficient computationl algorithm was developed for whole genome sgRNA design and off-target homology searches. By means of a dynamically constructed sequence-indexed database and a simplified sequence alignment method, this algorithm achieves very high efficiency while guaranteeing the identification of all existing potential off-target homologies. Via this algorithm, 1,876,775 sgRNAs were designed for the 19,153 human mRNA genes and only two sgRNAs were found to be free of off-target homology. By means of the novel and efficient sgRNA homology search algorithm introduced in this article, genome wide sgRNA design and off-target analysis were conducted and the results confirmed the mathematical analysis that for a sgRNA sequence, it is almost impossible to escape potential off-target homologies. Future innovations on the CRISPR Cas9 gene editing technology need to focus on how to eliminate the Cas9 off-target activity.

  18. In Vitro Evaluation of Molecular Tumor Targets in Nuclear Medicine: Immunohistochemistry Is One Option, but Under Which Conditions?

    Science.gov (United States)

    Reubi, Jean Claude

    2017-12-01

    The identification of new molecular targets for diagnostic and therapeutic applications using in vitro methods is an important challenge in nuclear medicine. One such method is immunohistochemistry, increasingly popular because it is easy to perform. This review presents the case for conducting receptor immunohistochemistry to evaluate potential molecular targets in human tumor tissue sections. The focus is on the immunohistochemistry of G-protein-coupled receptors, one of the largest families of cell surface proteins, representing a major class of drug targets and thus playing an important role in nuclear medicine. This review identifies common pitfalls and challenges and provides guidelines on performing such immunohistochemical studies. An appropriate validation of the target is a prerequisite for developing robust and informative new molecular probes. © 2017 by the Society of Nuclear Medicine and Molecular Imaging.

  19. A target-oriented data envelopment analysis for energy-environment efficiency improvement in Japan

    NARCIS (Netherlands)

    Suzuki, S.; Nijkamp, P.; Rietveld, P.

    2015-01-01

    This paper aims to offer a quantitative contribution to energy-environment policy in Japan in the aftermath of the Fukushima nuclear power accident. Since then, nuclear power energy supply has almost entirely been banned, and consequently, an intensive search for alternative forms of energy supply

  20. Efficient characterization of labeling uncertainty in closely-spaced targets tracking

    NARCIS (Netherlands)

    Moreno Leon, Carlos; Moreno Leon, Carlos; Driessen, Hans; Mandal, Pranab K.

    2016-01-01

    In this paper we propose a novel solution to the labeled multi-target tracking problem. The method presented is specially effective in scenarios where the targets have once moved in close proximity. When this is the case, disregarding the labeling uncertainty present in a solution (after the targets

  1. Development of a liquid tin microjet target for an efficient laser-produced plasma extreme ultraviolet source.

    Science.gov (United States)

    Higashiguchi, Takeshi; Hamada, Masaya; Kubodera, Shoichi

    2007-03-01

    A regenerative tin liquid microjet target was developed for a high average power extreme ultraviolet (EUV) source. The diameter of the target was smaller than 160 microm and good vacuum lower than 0.5 Pa was maintained during the operation. A maximum EUV conversion efficiency of 1.8% at the Nd:yttrium-aluminum-garnet laser intensity of around 2 x 10(11) Wcm(2) with a spot diameter of 175 microm (full width at half maximum) was observed. The angular distribution of the EUV emission remained almost isotropic, whereas suprathermal ions mainly emerged toward the target normal.

  2. Development of a liquid tin microjet target for an efficient laser-produced plasma extreme ultraviolet source

    Science.gov (United States)

    Higashiguchi, Takeshi; Hamada, Masaya; Kubodera, Shoichi

    2007-03-01

    A regenerative tin liquid microjet target was developed for a high average power extreme ultraviolet (EUV) source. The diameter of the target was smaller than 160 μm and good vacuum lower than 0.5 Pa was maintained during the operation. A maximum EUV conversion efficiency of 1.8% at the Nd:yttrium-aluminum-garnet laser intensity of around 2×1011 W/cm2 with a spot diameter of 175 μm (full width at half maximum) was observed. The angular distribution of the EUV emission remained almost isotropic, whereas suprathermal ions mainly emerged toward the target normal.

  3. Development of a liquid tin microjet target for an efficient laser-produced plasma extreme ultraviolet source

    International Nuclear Information System (INIS)

    Higashiguchi, Takeshi; Hamada, Masaya; Kubodera, Shoichi

    2007-01-01

    A regenerative tin liquid microjet target was developed for a high average power extreme ultraviolet (EUV) source. The diameter of the target was smaller than 160 μm and good vacuum lower than 0.5 Pa was maintained during the operation. A maximum EUV conversion efficiency of 1.8% at the Nd:yttrium-aluminum-garnet laser intensity of around 2x10 11 W/cm 2 with a spot diameter of 175 μm (full width at half maximum) was observed. The angular distribution of the EUV emission remained almost isotropic, whereas suprathermal ions mainly emerged toward the target normal

  4. Efficient Isothermal Titration Calorimetry Technique Identifies Direct Interaction of Small Molecule Inhibitors with the Target Protein.

    Science.gov (United States)

    Gal, Maayan; Bloch, Itai; Shechter, Nelia; Romanenko, Olga; Shir, Ofer M

    2016-01-01

    Protein-protein interactions (PPI) play a critical role in regulating many cellular processes. Finding novel PPI inhibitors that interfere with specific binding of two proteins is considered a great challenge, mainly due to the complexity involved in characterizing multi-molecular systems and limited understanding of the physical principles governing PPIs. Here we show that the combination of virtual screening techniques, which are capable of filtering a large library of potential small molecule inhibitors, and a unique secondary screening by isothermal titration calorimetry, a label-free method capable of observing direct interactions, is an efficient tool for finding such an inhibitor. In this study we applied this strategy in a search for a small molecule capable of interfering with the interaction of the tumor-suppressor p53 and the E3-ligase MDM2. We virtually screened a library of 15 million small molecules that were filtered to a final set of 80 virtual hits. Our in vitro experimental assay, designed to validate the activity of mixtures of compounds by isothermal titration calorimetry, was used to identify an active molecule against MDM2. At the end of the process the small molecule (4S,7R)-4-(4-chlorophenyl)-5-hydroxy-2,7-dimethyl-N-(6-methylpyridin-2-yl)-4,6,7,8 tetrahydrIoquinoline-3-carboxamide was found to bind MDM2 with a dissociation constant of ~2 µM. Following the identification of this single bioactive compound, spectroscopic measurements were used to further characterize the interaction of the small molecule with the target protein. 2D NMR spectroscopy was used to map the binding region of the small molecule, and fluorescence polarization measurement confirmed that it indeed competes with p53.

  5. Energy Efficient Sensor Scheduling with a Mobile Sink Node for the Target Tracking Application

    Directory of Open Access Journals (Sweden)

    Malin Premaratne

    2009-01-01

    Full Text Available Measurement losses adversely affect the performance of target tracking. The sensor network’s life span depends on how efficiently the sensor nodes consume energy. In this paper, we focus on minimizing the total energy consumed by the sensor nodes whilst avoiding measurement losses. Since transmitting data over a long distance consumes a significant amount of energy, a mobile sink node collects the measurements and transmits them to the base station. We assume that the default transmission range of the activated sensor node is limited and it can be increased to maximum range only if the mobile sink node is out-side the default transmission range. Moreover, the active sensor node can be changed after a certain time period. The problem is to select an optimal sensor sequence which minimizes the total energy consumed by the sensor nodes. In this paper, we consider two different problems depend on the mobile sink node’s path. First, we assume that the mobile sink node’s position is known for the entire time horizon and use the dynamic programming technique to solve the problem. Second, the position of the sink node is varied over time according to a known Markov chain, and the problem is solved by stochastic dynamic programming. We also present sub-optimal methods to solve our problem. A numerical example is presented in order to discuss the proposed methods’ performance.

  6. Cutting costs by achieving energy efficiency using monitoring, targeting and teamwork

    Energy Technology Data Exchange (ETDEWEB)

    Dittburner, D. [Unilever Canada, Toronto, ON (Canada)

    2004-07-01

    Unilever is a world leader of consumer goods with annual sales of $70 billion. This paper focuses on energy management projects developed at Unilever's Rexdale Plant, where 180 million pounds of edible oils and margarine are produced annually. The project is a response to corporate and market pressures to reduce costs. An overview of Unilever's relationship with Natural Resources Canada was provided. Results of the overall project were highlighted and included: $3 million in annual savings from increased efficiency in operations and equipment retrofits. An outline of the energy team at Unilever was presented, with their mission statement, rules and achievements, as well the company's overall goal of total productive manufacturing. A list of awards and financial incentives was presented, as well as details of financial savings incurred at the Rexdale Plant. Total energy reductions were presented, with a natural gas year to year comparison and utility to production ratios from 1999 to the present. A statement concerning the issue of corporate support by the vice president was provided. Seven steps to savings were presented, as well as details of the company's implementation of the steps. Details of the extended team involved in the project were provided, as well as extensive details about the employee awareness program instigated by the company, including a database of ideas achieved since 2001, as well as details of specific projects accomplished, with estimated savings for each project. An outline of the company's business model and methodology was presented, along with details of reduced costs, risk and improved management and communications. An outline of scoping studies was presented as well as a flow chart of projects and target-setting goals. Success factors were reviewed. Montage applications included energy management; performance contracting; energy forecasting; emissions and waste minimization; and cost allocation. tabs., figs.

  7. A numerical technique for enhanced efficiency and stability for the solution of the nuclear reactor equation

    International Nuclear Information System (INIS)

    Khotylev, V.A.; Hoogenboom, J.E.

    1996-01-01

    The paper presents new techniques for the solution of the nuclear reactor equation in diffusion approximation, that has enhanced efficiency and stability. The code system based on the new technique solves a number of steady-state and/or transient problems with coupled thermal hydraulics in one-, two-, or three dimensional geometry with reduced CPU time as compared to similar code systems of previous generations if well-posed neutronics problems are considered. Automated detection of ill-posed problem and selection of the appropriate numerical method makes the new code system capable of yielding a correct solution for wider range of problems without user intervention. (author)

  8. A numerical technique for enhanced efficiency and stability for the solution of the nuclear reactor equation

    Energy Technology Data Exchange (ETDEWEB)

    Khotylev, V.A.; Hoogenboom, J.E. [Delft Univ. of Technology, Interfaculty Reactor Inst., Delft (Netherlands)

    1996-07-01

    The paper presents new techniques for the solution of the nuclear reactor equation in diffusion approximation, that has enhanced efficiency and stability. The code system based on the new technique solves a number of steady-state and/or transient problems with coupled thermal hydraulics in one-, two-, or three dimensional geometry with reduced CPU time as compared to similar code systems of previous generations if well-posed neutronics problems are considered. Automated detection of ill-posed problem and selection of the appropriate numerical method makes the new code system capable of yielding a correct solution for wider range of problems without user intervention. (author)

  9. Remotex: a new concept for efficient remote operation and maintenance in nuclear fuel reprocessing

    International Nuclear Information System (INIS)

    Feldman, M.J.; White, J.R.

    1980-01-01

    Remotex is a concept of remote operation and maintenance that utilizes advanced manipulator design to improve plant operating efficiency, reduce personnel exposure, and improve safeguards and diversion resistance. It is a concept developed over the past two years in the conceptual design of the Hot Experimental Facility (HEF), a mechanically intense pilot plant facility designed to demonstrate reprocessng technology for early US breeder demonstration reactors. The Remotex concept is directly applicable to all segments of nuclear and nonnuclear industries where work tasks or conditions exist that are hazardous to the health of man

  10. Commonalty initiatives in US nuclear power plants to improve radiation protection culture and worker efficiency

    International Nuclear Information System (INIS)

    Wood, W.; Miller, D.

    2003-01-01

    Many US nuclear power plants have learned that common procedures, policies, instrumentation, tools and work practices achieve improvements to the radiation protection culture. Significant worker efficiency achievements are accomplished especially during refuelling outages. This paper discusses commonalty initiatives currently being implemented at many US Plants to address management challenges presented by deregulation of the US electric industry, reduction in the pool of outage contractors and aging of the experienced radiation worker population. The new INPO 2005 dose goals of 650 person-mSv/year for PWRs and 1200 person-mSv/yr for PWRs will require new approaches to radiation protection management to achieve these challenging goals by 2005. (authors)

  11. Nuclear cooperation targets global challenges. States back main pillars of the IAEA's work to strengthen nuclear safety, verification and technology transfer

    International Nuclear Information System (INIS)

    2000-01-01

    States meeting at the 44th IAEA General Conference in Vienna have set a challenging agenda for international nuclear cooperation into the 21st century that targets issues of global safety, security, and sustainable development. They adopted resolutions endorsing the Agency's programmes for strengthening activities under its three main pillars of work - nuclear verification, safety, and technology - that are closely linked to major challenges before the world. The document presents the main actions taken during the conference

  12. Application of high efficiency and reliable 3D-designed integral shrouded blades to nuclear turbines

    International Nuclear Information System (INIS)

    Watanabe, Eiichiro; Ohyama, Hiroharu; Tashiro, Hikaru; Sugitani, Toshiro; Kurosawa, Masaru

    1998-01-01

    Mitsubishi Heavy Industries, Ltd. has recently developed new blades for nuclear turbines, in order to achieve higher efficiency and higher reliability. The 3D aerodynamic design for 41 inch and 46 inch blades, their one piece structural design (integral-shrouded blades: ISB), and the verification test results using a model steam turbine are described in this paper. The predicted efficiency and lower vibratory stress have been verified. Based on these 60Hz ISB, 50Hz ISB series are under development using 'the law of similarity' without changing their thermodynamic performance and mechanical stress levels. Our 3D-designed reaction blades which are used for the high pressure and low pressure upstream stages, are also briefly mentioned. (author)

  13. Differences of detection efficiency among several nasal swab samples simulated for nuclear emergency accident

    International Nuclear Information System (INIS)

    Fukutsu, Kumiko; Yamada, Yuji; Kurihara, Osamu; Akashi, Makoto; Momose, Takumaro; Miyabe, Kenjiro

    2008-01-01

    At nuclear emergency accident such as inhalation intake of alpha nuclide, an indispensable nasal swab method has not been used for the precise internal dose estimation. One of the reasons is uncertainty in its radiation measurement, so that precise measurement with alpha spectrometry was examined for filter samples simulating nasal swab. It was confirmed that the alpha spectrometry made possible the distinction between solution and particulate in addition to the nuclide identification. The alpha activity in swab sample was precisely evaluated only when the detection efficiency was determined considering the self-absorption with filter fibers. Another big problem of wiping efficiency in nasal swabbing is still remain, but this study certainly raised the usefulness of the nasal swab method for rapid response in emergency. (author)

  14. Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects

    DEFF Research Database (Denmark)

    Lennen, Rebecca; Nilsson Wallin, Annika; Pedersen, Margit

    2016-01-01

    Homologous recombination of single-stranded oligonucleotides is a highly efficient process for introducing precise mutations into the genome of E. coli and other organisms when mismatch repair (MMR) is disabled. This can result in the rapid accumulation of off-target mutations that can mask desir...

  15. Receptor-targeted liposome-peptide-siRNA nanoparticles represent an efficient delivery system for MRTF silencing in conjunctival fibrosis.

    Science.gov (United States)

    Yu-Wai-Man, Cynthia; Tagalakis, Aristides D; Manunta, Maria D; Hart, Stephen L; Khaw, Peng T

    2016-02-24

    There is increasing evidence that the Myocardin-related transcription factor/Serum response factor (MRTF/SRF) pathway plays a key role in fibroblast activation and that knocking down MRTF can lead to reduced scarring and fibrosis. Here, we have developed a receptor-targeted liposome-peptide-siRNA nanoparticle as a non-viral delivery system for MRTF-B siRNA in conjunctival fibrosis. Using 50 nM siRNA, the MRTF-B gene was efficiently silenced by 76% and 72% with LYR and LER nanoparticles, respectively. The silencing efficiency was low when non-targeting peptides or siRNA alone or liposome-siRNA alone were used. LYR and LER nanoparticles also showed higher silencing efficiency than PEGylated LYR-P and LER-P nanoparticles. The nanoparticles were not cytotoxic using different liposomes, targeting peptides, and 50 nM siRNA. Three-dimensional fibroblast-populated collagen matrices were also used as a functional assay to measure contraction in vitro, and showed that MRTF-B LYR nanoparticles completely blocked matrix contraction after a single transfection treatment. In conclusion, this is the first study to develop and show that receptor-targeted liposome-peptide-siRNA nanoparticles represent an efficient and safe non-viral siRNA delivery system that could be used to prevent fibrosis after glaucoma filtration surgery and other contractile scarring conditions in the eye.

  16. Inhibition of CRM1-mediated nuclear export of influenza A nucleoprotein and nuclear export protein as a novel target for antiviral drug development

    Energy Technology Data Exchange (ETDEWEB)

    Chutiwitoonchai, Nopporn; Mano, Takafumi; Kakisaka, Michinori; Sato, Hirotaka [Viral Infectious Disease Unit, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan); Kondoh, Yasumitsu; Osada, Hiroyuki [Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan); Kotani, Osamu; Yokoyama, Masaru; Sato, Hironori [Laboratory of Viral Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011 (Japan); Aida, Yoko, E-mail: aida@riken.jp [Viral Infectious Disease Unit, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)

    2017-07-15

    An anti-influenza compound, DP2392-E10 based on inhibition of the nuclear export function of the viral nucleoprotein-nuclear export signal 3 (NP-NES3) domain was successfully identified by our previous high-throughput screening system. Here, we demonstrated that DP2392-E10 exerts its antiviral effect by inhibiting replication of a broad range of influenza A subtypes. In regard to the molecular mechanism, we revealed that DP2392-E10 inhibits nuclear export of both viral NP and nuclear export protein (NEP). More specifically, in vitro pull-down assays revealed that DP2392-E10 directly binds cellular CRM1, which mediates nuclear export of NP and NEP. In silico docking suggested that DP2392-E10 binds at a region close to the HEAT9 and HEAT10 domains of CRM1. Together, these results indicate that the CRM1-mediated nuclear export function of influenza virus represents a new potential target for antiviral drug development, and also provide a core structure for a novel class of inhibitors that target this function. - Highlights: •DP2392-E10 inhibits replication of a broad range of influenza A subtypes. •DP2392-E10 inhibits nuclear exports of NP and NEP via their NP-NES3 and NEP-NES2 domains, respectively. •DP2392-E10 is predicted to directly bind CRM1 in the region near the HEAT9 and HEAT10 repeats.

  17. Inhibition of CRM1-mediated nuclear export of influenza A nucleoprotein and nuclear export protein as a novel target for antiviral drug development

    International Nuclear Information System (INIS)

    Chutiwitoonchai, Nopporn; Mano, Takafumi; Kakisaka, Michinori; Sato, Hirotaka; Kondoh, Yasumitsu; Osada, Hiroyuki; Kotani, Osamu; Yokoyama, Masaru; Sato, Hironori; Aida, Yoko

    2017-01-01

    An anti-influenza compound, DP2392-E10 based on inhibition of the nuclear export function of the viral nucleoprotein-nuclear export signal 3 (NP-NES3) domain was successfully identified by our previous high-throughput screening system. Here, we demonstrated that DP2392-E10 exerts its antiviral effect by inhibiting replication of a broad range of influenza A subtypes. In regard to the molecular mechanism, we revealed that DP2392-E10 inhibits nuclear export of both viral NP and nuclear export protein (NEP). More specifically, in vitro pull-down assays revealed that DP2392-E10 directly binds cellular CRM1, which mediates nuclear export of NP and NEP. In silico docking suggested that DP2392-E10 binds at a region close to the HEAT9 and HEAT10 domains of CRM1. Together, these results indicate that the CRM1-mediated nuclear export function of influenza virus represents a new potential target for antiviral drug development, and also provide a core structure for a novel class of inhibitors that target this function. - Highlights: •DP2392-E10 inhibits replication of a broad range of influenza A subtypes. •DP2392-E10 inhibits nuclear exports of NP and NEP via their NP-NES3 and NEP-NES2 domains, respectively. •DP2392-E10 is predicted to directly bind CRM1 in the region near the HEAT9 and HEAT10 repeats.

  18. Two-dimensional multiplicity fluctuation analysis of target residues in nuclear collisions

    International Nuclear Information System (INIS)

    Dong-Hai, Zhang; Yao-Jie, Niu; Li-Chun, Wang; Wen-Jun, Yan; Li-Juan, Gao; Ming-Xing, Li; Li-Ping, Wu; Hui-Ling, Li; Jun-Sheng, Li

    2010-01-01

    Multiplicity fluctuation of the target residues emitted in the interactions in a wide range of projectile energies from 500 A MeV to 60 A GeV is investigated in the framework of two-dimensional scaled factorial moment methodology. The evidence of non-statistical multiplicity fluctuation is found in 16 O–AgBr collisions at 60 A GeV, but not in 56 Fe–AgBr collisions at 500 A MeV, 84 Kr–AgBr collisions at 1.7 A GeV, 16 O–AgBr collisions at 3.7 A GeV and 197 Au–AgBr collisions at 10.7 A GeV. (nuclear physics)

  19. The critical role of the industrial sector in reaching long-term emission reduction, energy efficiency and renewable targets

    International Nuclear Information System (INIS)

    Fais, Birgit; Sabio, Nagore; Strachan, Neil

    2016-01-01

    Highlights: • A new industrial modelling approach in a whole energy systems model is developed. • The contribution of UK industry to long-term energy policy targets is analysed. • Emission reductions of up to 77% can be achieved in the UK industry until 2050. • The UK industry sector is essential for achieving the overall efficiency commitments. • UK industry can make a moderate contribution to the expansion of renewable energies. - Abstract: This paper evaluates the critical contribution of the industry sector to long-term decarbonisation, efficiency and renewable energy policy targets. Its methodological novelty is the incorporation of a process-oriented modelling approach based on a comprehensive technology database for the industry sector in a national energy system model for the UK (UKTM), allowing quantification of the role of both decarbonisation of upstream energy vectors and of mitigation options in the industrial sub-categories. This enhanced model is then applied in a comparative policy scenario analysis that explores various target dimensions on emission mitigation, renewable energy and energy efficiency at both a national and European level. The results show that ambitious emission cuts in the industry sector of up to 77% until 2050 compared to 2010 can be achieved. Moreover, with a reduction in industrial energy demand of up to 31% between 2010 and 2050, the sector is essential for achieving the overall efficiency commitments. The industry sector also makes a moderate contribution to the expansion of renewable energies mostly through the use of biomass for low-temperature heating services. However, additional sub-targets on renewable sources and energy efficiency need to be assessed critically, as they can significantly distort the cost-efficiency of the long-term mitigation pathway.

  20. Evaluation of Efficiency Activities in the Industrial Sector Undertaken in Response to Greenhouse Gas Emission Reduction Targets

    Energy Technology Data Exchange (ETDEWEB)

    Price, Lynn; de la Rue du Can, Stephane; Lu, Hongyou; Horvath, Arpad

    2010-05-21

    The 2006 California Global Warming Solutions Act calls for reducing greenhouse gas (GHG) emissions to 1990 levels by 2020. Meeting this target will require action from all sectors of the California economy, including industry. The industrial sector consumes 25% of the energy used and emits 28% of the carbon dioxide (CO{sub 2}) produced in the state. Many countries around the world have national-level GHG reduction or energy-efficiency targets, and comprehensive programs focused on implementation of energy efficiency and GHG emissions mitigation measures in the industrial sector are essential for achieving their goals. A combination of targets and industry-focused supporting programs has led to significant investments in energy efficiency as well as reductions in GHG emissions within the industrial sectors in these countries. This project has identified program and policies that have effectively targeted the industrial sector in other countries to achieve real energy and CO{sub 2} savings. Programs in Ireland, France, The Netherlands, Denmark, and the UK were chosen for detailed review. Based on the international experience documented in this report, it is recommended that companies in California's industrial sector be engaged in a program to provide them with support to meet the requirements of AB32, The Global Warming Solution Act. As shown in this review, structured programs that engage industry, require members to evaluate their potential efficiency measures, plan how to meet efficiency or emissions reduction goals, and provide support in achieving the goals, can be quite effective at assisting companies to achieve energy efficiency levels beyond those that can be expected to be achieved autonomously.

  1. Plant parasitic nematode effectors target host defence and nuclear functions to establish feeding cells

    Directory of Open Access Journals (Sweden)

    Michaël eQuentin

    2013-03-01

    Full Text Available Plant parasitic nematodes are microscopic worms, the most damaging species of which have adopted a sedentary lifestyle within their hosts. These obligate endoparasites have a biotrophic relationship with plants, in which they induce the differentiation of root cells into hypertrophied, multinucleate feeding cells. Effectors synthesised in the oesophageal glands of the nematode are injected into the plant cells via the syringe-like stylet and play a key role in manipulating the host machinery. The establishment of specialized feeding cells requires these effectors to modulate many aspects of plant cell morphogenesis and physiology, including defence responses. This cell reprogramming requires changes to host nuclear processes. Some proteins encoded by parasitism genes target host nuclei. Several of these proteins were immunolocalised within feeding cell nuclei or shown to interact with host nuclear proteins. Comparative genomics and functional analyses are gradually revealing the roles of nematode effectors. We describe here these effectors and their hypothesised roles in the unique feeding behaviour of these pests.

  2. Setting SMART targets for industrial energy use and industrial energy efficiency

    NARCIS (Netherlands)

    Rietbergen, M.G.|info:eu-repo/dai/nl/14111634X; Blok, K.|info:eu-repo/dai/nl/07170275X

    2010-01-01

    Industrial energy policies often require the setting of quantitative targets to reduce energy use and/or greenhouse gas emissions. In this paper a taxonomy has been developed for categorizing SMART industrial energy use or greenhouse gas emission reduction targets. The taxonomy includes volume

  3. Targeting energy justice: Exploring spatial, racial/ethnic and socioeconomic disparities in urban residential heating energy efficiency

    International Nuclear Information System (INIS)

    Reames, Tony Gerard

    2016-01-01

    Fuel poverty, the inability of households to afford adequate energy services, such as heating, is a major energy justice concern. Increasing residential energy efficiency is a strategic fuel poverty intervention. However, the absence of easily accessible household energy data impedes effective targeting of energy efficiency programs. This paper uses publicly available data, bottom-up modeling and small-area estimation techniques to predict the mean census block group residential heating energy use intensity (EUI), an energy efficiency proxy, in Kansas City, Missouri. Results mapped using geographic information systems (GIS) and statistical analysis, show disparities in the relationship between heating EUI and spatial, racial/ethnic, and socioeconomic block group characteristics. Block groups with lower median incomes, a greater percentage of households below poverty, a greater percentage of racial/ethnic minority headed-households, and a larger percentage of adults with less than a high school education were, on average, less energy efficient (higher EUIs). Results also imply that racial segregation, which continues to influence urban housing choices, exposes Black and Hispanic households to increased fuel poverty vulnerability. Lastly, the spatial concentration and demographics of vulnerable block groups suggest proactive, area- and community-based targeting of energy efficiency assistance programs may be more effective than existing self-referral approaches. - Highlights: • Develops statistical model to predict block group (BG) residential heating energy use intensity (EUI), an energy efficiency proxy. • Bivariate and multivariate analyses explore racial/ethnic and socioeconomic relationships with heating EUI. • BGs with more racial/ethnic minority households had higher heating EUI. • BGs with lower socioeconomics had higher heating EUI. • Mapping heating EUI can facilitate effective energy efficiency intervention targeting.

  4. Computation of full energy peak efficiency for nuclear power plant radioactive plume using remote scintillation gamma-ray spectrometry.

    Science.gov (United States)

    Grozdov, D S; Kolotov, V P; Lavrukhin, Yu E

    2016-04-01

    A method of full energy peak efficiency estimation in the space around scintillation detector, including the presence of a collimator, has been developed. It is based on a mathematical convolution of the experimental results with the following data extrapolation. The efficiency data showed the average uncertainty less than 10%. Software to calculate integral efficiency for nuclear power plant plume was elaborated. The paper also provides results of nuclear power plant plume height estimation by analysis of the spectral data. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Inclusive characteristics of the nuclear target fragmentation products induced by relativistic particles

    International Nuclear Information System (INIS)

    Bogatin, V.I.; Ganza, E.A.; Lozhkin, O.V.; Murin, Yu.A.; Oplavin, V.S.; Perfilov, N.A.; Yakovlev, Yu.P.

    1981-01-01

    An experimental investigation of inclusive characteristics of nuclei-target fragmentation is conducted for further development and test of physical value of the earlier suggested nuclear fragmentation model based on the connection of the fragmentation with fluctuations of the quasiparticle density in the two-component quantum liquid, an experimental investigation of the inclusive characteristics of the nuclei-target fragmentation is carried out. The processes of sup(3, 4, 6, 8)He and sup(6, 7, 8, 9, 11)Li fragment formation during the interaction of relativistic protons (Esub(p)=6.7 GeV) and deutrons (Esub(d)=3.1 GeV) with 112 Sn and 124 Sn isotopes are studied by the method of semiconductive ΔE-E detectors. Differential energy spectra of fragments and isotopic ratio of cross sections of their formation as well as data on the dependence of isotopic ratios of fragmentation cross sections on the energy of incident particles and on the fragment energy are obtained. Presented is a phenomenological model of fragmentation within the frames of which the obtained experimental data are analyzed [ru

  6. New supersonic gas jet target for low energy nuclear reaction studies

    Science.gov (United States)

    Favela, F.; Acosta, L.; Andrade, E.; Araujo, V.; Huerta, A.; de Lucio, O. G.; Murillo, G.; Ortiz, M. E.; Policroniades, R.; Santa Rita, P.; Varela, A.; Chávez, E.

    2015-12-01

    A windowless supersonic gas jet target (SUGAR) has been put in operation recently in Mexico. It is the first target of its kind in the country and the region. New research opportunities become available with this facility through the study of the direct beam-gas interaction: nuclear physics and astrophysics, atomic physics, interaction of radiation with matter and other interdisciplinary applications. A general description of the apparatus and its commissioning is given here. Air, nitrogen and argon jets were produced. Proton and deuteron beams were used to measure key parameters of the system to compare with theoretical estimates. In addition, as a first study case, we present data from the 14N (d ,α )12C reaction, at center of mass energies between 1.9 and 3.0 MeV with an E-Δ E telescope detector at 35°. Excitation functions for several excited states were constructed and an 16O resonance at 22.72 MeV was confirmed.

  7. Diesel engine power generating set efficiency for nuclear power plant electrical energy supply in case of emergency

    International Nuclear Information System (INIS)

    Popovic, I.; Aksamija, R.; Tumpa, M.

    1984-01-01

    Working ability of diesel engine set to starting and functioning reliability during operation is evaluated within study of efficiency. methods of calculation are applied: data compilation method and Markov method. The evaluation is that a diesel engine set has efficiency of 0.993285. It is a high efficiency which ensures a safe start, load take over and safe operation. This evaluation makes a basis for similar calculations which will be needed for national nuclear program. (author)

  8. GTHTR300—A nuclear power plant design with 50% generating efficiency

    International Nuclear Information System (INIS)

    Sato, Hiroyuki; Yan, Xing L.; Tachibana, Yukio; Kunitomi, Kazuhiko

    2014-01-01

    Highlights: • GTHTR300 reported 10 years ago is updated. • Cycle and reactor core designs as well as turbine blade material are improved. • The study showed that GTHTR300 is able to yield a net plant efficiency of 50.4%. - Abstract: GTHTR300 is a gas turbine high temperature reactor power generation plant design. The baseline design reported by Japan Atomic Energy Agency a decade ago attained 45.6% net efficiency. Technological improvements have since been made that make further increase in efficiency practical: first, the cycle parameters are upgraded by utilizing the newly acquired design data including those from component tests. Next, the core design is optimized to raise the reactor outlet coolant temperature from the baseline of 850 °C to the level of 950 °C demonstrated on the long-term test reactor operation. Both core physics and thermal hydraulics are investigated to demonstrate the corresponding temperature rise is within the design limit so that the existing fuel design can continue to apply. Finally, an advanced type of turbine blade material that has only recently entered in commercial service in aircraft engine is found to be useable for this design to realize a turbine inlet temperature of 950 °C without requiring blade cooling. As detailed in this paper, these design improvements result in a nearly 5% gain in overall plant efficiency and enable the GTHTR300 to break the 50% efficiency barrier of nuclear plant while using only the existing technologies. This result is expected to contribute to the early market deployment of high temperature gas-cooled reactor

  9. GTHTR300—A nuclear power plant design with 50% generating efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Hiroyuki, E-mail: sato.hiroyuki09@jaea.go.jp; Yan, Xing L.; Tachibana, Yukio; Kunitomi, Kazuhiko

    2014-08-15

    Highlights: • GTHTR300 reported 10 years ago is updated. • Cycle and reactor core designs as well as turbine blade material are improved. • The study showed that GTHTR300 is able to yield a net plant efficiency of 50.4%. - Abstract: GTHTR300 is a gas turbine high temperature reactor power generation plant design. The baseline design reported by Japan Atomic Energy Agency a decade ago attained 45.6% net efficiency. Technological improvements have since been made that make further increase in efficiency practical: first, the cycle parameters are upgraded by utilizing the newly acquired design data including those from component tests. Next, the core design is optimized to raise the reactor outlet coolant temperature from the baseline of 850 °C to the level of 950 °C demonstrated on the long-term test reactor operation. Both core physics and thermal hydraulics are investigated to demonstrate the corresponding temperature rise is within the design limit so that the existing fuel design can continue to apply. Finally, an advanced type of turbine blade material that has only recently entered in commercial service in aircraft engine is found to be useable for this design to realize a turbine inlet temperature of 950 °C without requiring blade cooling. As detailed in this paper, these design improvements result in a nearly 5% gain in overall plant efficiency and enable the GTHTR300 to break the 50% efficiency barrier of nuclear plant while using only the existing technologies. This result is expected to contribute to the early market deployment of high temperature gas-cooled reactor.

  10. Tailoring cross-sectional energy-efficiency measures to target groups in industry

    NARCIS (Netherlands)

    Wohlfarth, Katharina; Eichhammer, Wolfgang; Schlomann, Barbara; Worrell, Ernst

    2018-01-01

    The improvement of energy efficiency in industrial companies plays a crucial role for the energy transition. Although significant economic potentials have been identified, the concerned actors are still struggling to realize them fully. To support the implementation of energy efficiency measures by

  11. On the efficiency calibration of Si(Li) detector in the low-energy region using thick-target bremsstrahlung

    Energy Technology Data Exchange (ETDEWEB)

    An, Z. E-mail: anzhu@scu.edu.cn; Liu, M.T

    2002-10-01

    In this paper, the efficiency calibration of a Si(Li) detector in the low-energy region down to 0.58 keV has been performed using thick-carbon-target bremsstrahlung by 19 keV electron impact. The shape of the efficiency calibration curve was determined from the thick-carbon-target bremsstrahlung spectrum, and the absolute value for the efficiency calibration was obtained from the use of {sup 241}Am radioactive standard source. The modified Wentzel's formula for thick-target bremsstrahlung was employed and it was also compared with the most recently developed theoretical model based upon the doubly differential cross-sections for bremsstrahlung of Kissel, Quarles and Pratt. In the present calculation of theoretical bremsstrahlung, the self-absorption correction and the convolution of detector's response function with the bremsstrahlung spectrum have simultaneously been taken into account. The accuracy for the efficiency calibration in the low-energy region with the method described here was estimated to be about 6%. Moreover, the self-absorption correction calculation based upon the prescription of Wolters et al. has also been presented as an analytical factor with the accuracy of {approx}1%.

  12. Better Targeting, Better Efficiency for Wide-Scale Neuronal Transduction with the Synapsin Promoter and AAV-PHP.B.

    Science.gov (United States)

    Jackson, Kasey L; Dayton, Robert D; Deverman, Benjamin E; Klein, Ronald L

    2016-01-01

    Widespread genetic modification of cells in the central nervous system (CNS) with a viral vector has become possible and increasingly more efficient. We previously applied an AAV9 vector with the cytomegalovirus/chicken beta-actin (CBA) hybrid promoter and achieved wide-scale CNS transduction in neonatal and adult rats. However, this method transduces a variety of tissues in addition to the CNS. Thus we studied intravenous AAV9 gene transfer with a synapsin promoter to better target the neurons. We noted in systematic comparisons that the synapsin promoter drives lower level expression than does the CBA promoter. The engineered adeno-associated virus (AAV)-PHP.B serotype was compared with AAV9, and AAV-PHP.B did enhance the efficiency of expression. Combining the synapsin promoter with AAV-PHP.B could therefore be advantageous in terms of combining two refinements of targeting and efficiency. Wide-scale expression was used to model a disease with widespread pathology. Vectors encoding the amyotrophic lateral sclerosis (ALS)-related protein transactive response DNA-binding protein, 43 kDa (TDP-43) with the synapsin promoter and AAV-PHP.B were used for efficient CNS-targeted TDP-43 expression. Intracerebroventricular injections were also explored to limit TDP-43 expression to the CNS. The neuron-selective promoter and the AAV-PHP.B enhanced gene transfer and ALS disease modeling in adult rats.

  13. Better Targeting, Better Efficiency for Wide-scale Neuronal Transduction with the Synapsin Promoter and AAV-PHP.B

    Directory of Open Access Journals (Sweden)

    Kasey L Jackson

    2016-11-01

    Full Text Available Widespread genetic modification of cells in the central nervous system (CNS with a viral vector has become possible and increasingly more efficient. We previously applied an AAV9 vector with the cytomegalovirus/chicken beta-actin hybrid (CBA promoter and achieved wide-scale CNS transduction in neonatal and adult rats. However, this method transduces a variety of tissues in addition to the CNS. Thus we studied intravenous AAV9 gene transfer with a synapsin promoter to better target the neurons. We noted in systematic comparisons that the synapsin promoter drives lower level expression than does the CBA promoter. The engineered AAV-PHP.B serotype was compared with AAV9, and AAV-PHP.B did enhance the efficiency of expression. Combining the synapsin promoter with AAV-PHP.B could therefore be advantageous in terms of combining two refinements of targeting and efficiency. Wide-scale expression was used to model a disease with widespread pathology. Vectors encoding the amyotrophic lateral sclerosis (ALS-related protein TDP-43 with the synapsin promoter and AAV-PHP.B were used for efficient CNS-targeted TDP-43 expression. Intracerebroventricular injections were also explored to limit TDP-43 expression to the CNS. The neuron-selective promoter and the AAV-PHP.B enhanced gene transfer and ALS disease modeling in adult rats.

  14. International target values 2000 for measurement uncertainties in safeguarding nuclear materials

    International Nuclear Information System (INIS)

    Aigner, H.; Binner, R.; Kuhn, E.

    2001-01-01

    The IAEA has prepared a revised and updated version of International Target Values (ITVs) for uncertainty components in measurements of nuclear material. The ITVs represent uncertainties to be considered in judging the reliability of analytical techniques applied to industrial nuclear and fissile material subject to safeguards verification. The tabulated values represent estimates of the 'state of the practice' which ought to be achievable under routine conditions by adequately equipped, experienced laboratories. The ITVs 2000 are intended to be used by plant operators and safeguards organizations as a reference of the quality of measurements achievable in nuclear material accountancy, and for planning purposes. The IAEA prepared a draft of a technical report presenting the proposed ITVs 2000, and in April 2000 the chairmen or officers of the panels or organizations listed below were invited to co- author the report and to submit the draft to a discussion by their panels and organizations. Euratom Safeguards Inspectorate, ESAKDA Working Group on Destructive Analysis, ESARDA Working Group on Non Destructive Analysis, Institute of Nuclear Material Management, Japanese Expert Group on ITV-2000, ISO Working Group on Analyses in Spent Fuel Reprocessing, ISO Working Group on Analyses in Uranium Fuel Fabrication, ISO Working Group on Analyses in MOX Fuel Fabrication, Agencia Brasileno-Argentina de Contabilidad y Control de Materiales Nucleares (ABACC). Comments from the above groups were received and incorporated into the final version of the document, completed in April 2001. The ITVs 2000 represent target standard uncertainties, expressing the precision achievable under stipulated conditions. These conditions typically fall in one of the two following categories: 'repeatability conditions' normally encountered during the measurements done within one inspection period; or 'reproducibility conditions' involving additional sources of measurement variability such as

  15. Computationally Efficient Automatic Coast Mode Target Tracking Based on Occlusion Awareness in Infrared Images.

    Science.gov (United States)

    Kim, Sohyun; Jang, Gwang-Il; Kim, Sungho; Kim, Junmo

    2018-03-27

    This paper proposes the automatic coast mode tracking of centroid trackers for infrared images to overcome the target occlusion status. The centroid tracking method, using only the brightness information of an image, is still widely used in infrared imaging tracking systems because it is difficult to extract meaningful features from infrared images. However, centroid trackers are likely to lose the track because they are highly vulnerable to screened status by the clutter or background. Coast mode, one of the tracking modes, maintains the servo slew rate with the tracking rate right before the loss of track. The proposed automatic coast mode tracking method makes decisions regarding entering coast mode by the prediction of target occlusion and tries to re-lock the target and resume the tracking after blind time. This algorithm comprises three steps. The first step is the prediction process of the occlusion by checking both matters which have target-likelihood brightness and which may screen the target despite different brightness. The second step is the process making inertial tracking commands to the servo. The last step is the process of re-locking a target based on the target modeling of histogram ratio. The effectiveness of the proposed algorithm is addressed by presenting experimental results based on computer simulation with various test imagery sequences compared to published tracking algorithms. The proposed algorithm is tested under a real environment with a naval electro-optical tracking system (EOTS) and airborne EO/IR system.

  16. Computationally Efficient Automatic Coast Mode Target Tracking Based on Occlusion Awareness in Infrared Images

    Directory of Open Access Journals (Sweden)

    Sohyun Kim

    2018-03-01

    Full Text Available This paper proposes the automatic coast mode tracking of centroid trackers for infrared images to overcome the target occlusion status. The centroid tracking method, using only the brightness information of an image, is still widely used in infrared imaging tracking systems because it is difficult to extract meaningful features from infrared images. However, centroid trackers are likely to lose the track because they are highly vulnerable to screened status by the clutter or background. Coast mode, one of the tracking modes, maintains the servo slew rate with the tracking rate right before the loss of track. The proposed automatic coast mode tracking method makes decisions regarding entering coast mode by the prediction of target occlusion and tries to re-lock the target and resume the tracking after blind time. This algorithm comprises three steps. The first step is the prediction process of the occlusion by checking both matters which have target-likelihood brightness and which may screen the target despite different brightness. The second step is the process making inertial tracking commands to the servo. The last step is the process of re-locking a target based on the target modeling of histogram ratio. The effectiveness of the proposed algorithm is addressed by presenting experimental results based on computer simulation with various test imagery sequences compared to published tracking algorithms. The proposed algorithm is tested under a real environment with a naval electro-optical tracking system (EOTS and airborne EO/IR system.

  17. The Gpn3 Q279* cancer-associated mutant inhibits Gpn1 nuclear export and is deficient in RNA polymerase II nuclear targeting.

    Science.gov (United States)

    Barbosa-Camacho, Angel A; Méndez-Hernández, Lucía E; Lara-Chacón, Bárbara; Peña-Gómez, Sonia G; Romero, Violeta; González-González, Rogelio; Guerra-Moreno, José A; Robledo-Rivera, Angélica Y; Sánchez-Olea, Roberto; Calera, Mónica R

    2017-11-01

    Gpn3 is required for RNA polymerase II (RNAPII) nuclear targeting. Here, we investigated the effect of a cancer-associated Q279* nonsense mutation in Gpn3 cellular function. Employing RNAi, we replaced endogenous Gpn3 by wt or Q279* RNAi-resistant Gpn3R in epithelial model cells. RNAPII nuclear accumulation and transcriptional activity were markedly decreased in cells expressing only Gpn3R Q279*. Wild-type Gpn3R localized to the cytoplasm but a fraction of Gpn3R Q279* entered the cell nucleus and inhibited Gpn1-EYFP nuclear export. This property and the transcriptional deficit in Gpn3R Q279*-expressing cells required a PDZ-binding motif generated by the Q279* mutation. We conclude that an acquired PDZ-binding motif in Gpn3 Q279* caused Gpn3 nuclear entry, and inhibited Gpn1 nuclear export and Gpn3-mediated RNAPII nuclear targeting. © 2017 Federation of European Biochemical Societies.

  18. Inhibition of CRM1-mediated nuclear export of influenza A nucleoprotein and nuclear export protein as a novel target for antiviral drug development.

    Science.gov (United States)

    Chutiwitoonchai, Nopporn; Mano, Takafumi; Kakisaka, Michinori; Sato, Hirotaka; Kondoh, Yasumitsu; Osada, Hiroyuki; Kotani, Osamu; Yokoyama, Masaru; Sato, Hironori; Aida, Yoko

    2017-07-01

    An anti-influenza compound, DP2392-E10 based on inhibition of the nuclear export function of the viral nucleoprotein-nuclear export signal 3 (NP-NES3) domain was successfully identified by our previous high-throughput screening system. Here, we demonstrated that DP2392-E10 exerts its antiviral effect by inhibiting replication of a broad range of influenza A subtypes. In regard to the molecular mechanism, we revealed that DP2392-E10 inhibits nuclear export of both viral NP and nuclear export protein (NEP). More specifically, in vitro pull-down assays revealed that DP2392-E10 directly binds cellular CRM1, which mediates nuclear export of NP and NEP. In silico docking suggested that DP2392-E10 binds at a region close to the HEAT9 and HEAT10 domains of CRM1. Together, these results indicate that the CRM1-mediated nuclear export function of influenza virus represents a new potential target for antiviral drug development, and also provide a core structure for a novel class of inhibitors that target this function. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. The RNA Exosome Adaptor ZFC3H1 Functionally Competes with Nuclear Export Activity to Retain Target Transcripts

    DEFF Research Database (Denmark)

    Silla, Toomas; Karadoulama, Evdoxia; Mąkosa, Dawid

    2018-01-01

    , containing polyadenylated (pA+) RNA secluded from nucleocytoplasmic export. We asked whether exosome co-factors could serve such nuclear retention. Co-localization studies revealed the enrichment of pA+ RNA foci with "pA-tail exosome targeting (PAXT) connection" components MTR4, ZFC3H1, and PABPN1......Mammalian genomes are promiscuously transcribed, yielding protein-coding and non-coding products. Many transcripts are short lived due to their nuclear degradation by the ribonucleolytic RNA exosome. Here, we show that abolished nuclear exosome function causes the formation of distinct nuclear foci...... but no overlap with known nuclear structures such as Cajal bodies, speckles, paraspeckles, or nucleoli. Interestingly, ZFC3H1 is required for foci formation, and in its absence, selected pA+ RNAs, including coding and non-coding transcripts, are exported to the cytoplasm in a process dependent on the mRNA export...

  20. A pre-protective strategy for precise tumor targeting and efficient photodynamic therapy with a switchable DNA/upconversion nanocomposite.

    Science.gov (United States)

    Yu, Zhengze; Ge, Yegang; Sun, Qiaoqiao; Pan, Wei; Wan, Xiuyan; Li, Na; Tang, Bo

    2018-04-14

    Tumor-specific targeting based on folic acid (FA) is one of the most common and significant approaches in cancer therapy. However, the expression of folate receptors (FRs) in normal tissues will lead to unexpected targeting and unsatisfactory therapeutic effect. To address this issue, we develop a pre-protective strategy for precise tumor targeting and efficient photodynamic therapy (PDT) using a switchable DNA/upconversion nanocomposite, which can be triggered in the acidic tumor microenvironment. The DNA/upconversion nanocomposite is composed of polyacrylic acid (PAA) coated upconversion nanoparticles (UCNPs), the surface of which is modified using FA and chlorin e6 (Ce6) functionalized DNA sequences with different lengths. Initially, FA on the shorter DNA was protected by a longer DNA to prevent the bonding to FRs on normal cells. Once reaching the acidic tumor microenvironment, C base-rich longer DNA forms a C-quadruplex, resulting in the exposure of the FA groups and the bonding of FA and FRs on cancer cell membranes to achieve precise targeting. Simultaneously, the photosensitizer chlorin e6 (Ce6) gets close to the surface of UCNPs, enabling the excitation of Ce6 to generate singlet oxygen ( 1 O 2 ) under near infrared light via Förster resonance energy transfer (FRET). In vivo experiments indicated that higher tumor targeting efficiency was achieved and the tumor growth was greatly inhibited through the pre-protective strategy.

  1. Electricity is the real target - nuclear energy the scapegoat. Comparison between nuclear energy and hydro power voting behaviour

    International Nuclear Information System (INIS)

    Aegerter, Irene

    1993-01-01

    As nuclear community sometimes feel desperate because the nuclear energy is a very special subject triggering so much controversy among women and young persons especially it has been found that the battle against nuclear energy is just a pretext. Comparing the campaign on a referendum against hydropower - voted in Switzerland in may 1992 it was found astonishingly that exactly the same arguments were applied as during the campaign for the phase out of nuclear energy in 1990. The results were presented at PIME 1991. Voting behaviour for nuclear energy and hydro power are comparable: the gender gap (32% acceptance by men versus 48% by women) found in the 1992 vote about stopping hydropower plants in Switzerland was bigger than the one found in the 1990 vote about nuclear energy. A detailed analysis of these data is presented

  2. Efficient Committed Budget for Implementing Target Audit Probability for Many Inspectees

    OpenAIRE

    Yim, Andrew

    2009-01-01

    Strategic models of auditor-inspectee interaction have neglected implementation details in multiple-inspectee settings. With multiple inspectees, the target audit probability derived from the standard analysis can be implemented with sampling plans differing in the budgets committed to support them. Overly committed audit budgets tie up unneeded resources that could have been allocated for better uses. This paper studies the minimum committed budget required to implement a target audit probab...

  3. Insertion of a nuclear factor kappa B DNA nuclear-targeting sequence potentiates suicide gene therapy efficacy in lung cancer cell lines

    DEFF Research Database (Denmark)

    Cramer, F; Christensen, C L; Poulsen, T T

    2012-01-01

    Lung cancer currently causes the majority of cancer-related deaths worldwide and new treatments are in high demand. Gene therapy could be a promising treatment but currently lacks sufficient efficiency for clinical use, primarily due to limited cellular and nuclear DNA delivery. In the present...

  4. TALE nickase mediates high efficient targeted transgene integration at the human multi-copy ribosomal DNA locus.

    Science.gov (United States)

    Wu, Yong; Gao, Tieli; Wang, Xiaolin; Hu, Youjin; Hu, Xuyun; Hu, Zhiqing; Pang, Jialun; Li, Zhuo; Xue, Jinfeng; Feng, Mai; Wu, Lingqian; Liang, Desheng

    2014-03-28

    Although targeted gene addition could be stimulated strikingly by a DNA double strand break (DSB) created by either zinc finger nucleases (ZFNs) or TALE nucleases (TALENs), the DSBs are really mutagenic and toxic to human cells. As a compromised solution, DNA single-strand break (SSB) or nick has been reported to mediate high efficient gene addition but with marked reduction of random mutagenesis. We previously demonstrated effective targeted gene addition at the human multicopy ribosomal DNA (rDNA) locus, a genomic safe harbor for the transgene with therapeutic potential. To improve the transgene integration efficiency by using TALENs while lowering the cytotoxicity of DSBs, we created both TALENs and TALE nickases (TALENickases) targeting this multicopy locus. A targeting vector which could integrate a GFP cassette at the rDNA locus was constructed and co-transfected with TALENs or TALENickases. Although the fraction of GFP positive cells using TALENs was greater than that using TALENickases during the first few days after transfection, it reduced to a level less than that using TALENickases after continuous culture. Our findings showed that the TALENickases were more effective than their TALEN counterparts at the multi-copy rDNA locus, though earlier studies using ZFNs and ZFNickases targeting the single-copy loci showed the reverse. Besides, TALENickases mediated the targeted integration of a 5.4 kb fragment at a frequency of up to 0.62% in HT1080 cells after drug selection, suggesting their potential application in targeted gene modification not being limited at the rDNA locus. Copyright © 2014 Elsevier Inc. All rights reserved.

  5. Theory of error for target factor analysis with applications to mass spectrometry and nuclear magnetic resonance spectrometry

    International Nuclear Information System (INIS)

    Malinowski, E.R.

    1978-01-01

    Based on the theory of error for abstract factor analysis described earlier, a theory of error for target factor analysis is developed. The theory shows how the error in the data matrix mixes with the error in the target test vector. The apparent error in a target test is found to be a vector sum of the real error in the target vector and the real error in the predicted vector. The theory predicts the magnitudes of these errors without requiring any a priori knowledge of the error in the data matrix or the target vector. A reliability function and a spoil function are developed for the purpose of assessing the validity and the worthiness of a target vector. Examples from model data, mass spectrometry and nuclear magnetic resonance spectrometry are presented. (Auth.)

  6. Versatile and Efficient Targeting Using a Single Nanoparticulate Platform: Application to Cancer and Alzheimer's Disease

    NARCIS (Netherlands)

    Le Droumaguet, Benjamin; Nicolas, Julien; Brambilla, Davide; Mura, Simona; Maksimenko, Andrei; de Kimpe, Line; Salvati, Elisa; Zona, Cristiano; Airoldi, Cristina; Canovi, Mara; Gobbi, Marco; Magali, Noiray; La Ferla, Barbara; Nicotra, Francesco; Scheper, Wiep; Flores, Orfeu; Masserini, Massimo; Andrieux, Karine; Couvreur, Patrick

    2012-01-01

    A versatile and efficient functionalization strategy for polymeric nanoparticles (NPs) has been reported and successfully applied to PEGylated, biodegradable poly(alkyl cyanoacrylate) (PACA) nanocarriers. The relevance of this platform was demonstrated in both the fields of cancer and Alzheimer's

  7. Economic efficiency of Nuclear Cell Mars with reference to different regions with the account cogenerating production

    International Nuclear Information System (INIS)

    Alekseev, P. N.; Kucharkin, N. E.; Udjanskiy, Y. N.; Schepetina, T. D.; Subbotin, S. A.

    2004-01-01

    The popular belief in a low economic efficiency NPP with nuclear reactors of small capacity (SNPP) is stipulated by the stereotyped approach to their role as a power source (PS) and absence of a system approach to an estimation of their role and place in economy of regions. Actually, the specific expenses of installed capacity for SNPP can be some times higher than those for ones with high-power reactors. As a rule, pay back of the SNPP projects is justified proceeding from only the income of sale produced electric power and heat. Poor economic efficiency of such variant of use forces the developers to consider variants cogeneration of useful production, for example, power-desalination complexes. But thus it is not taken into account, that only nuclear power source (NPS), due to quality of long-term autonomy can ensure in hard-to-reach regions ecologically safe, practically unbounded on time, reliable energy provision of unique production manufacture, which can be yielded only in a sectional place and due to presence of reliable and ecologically acceptable power supply. Examples of such exclusive symbiosis of technologies can be enough, especially taking into account the factor of an ecological acceptability, which acquires the increasing weight at definition of competitiveness of the projects. The factor of uniqueness at technologies combination in a sectional context does not contradict the strategy of seriality small capacity NPS application, as they are irreplaceable for long-time and reliable power supply of the independent removed or hard-to-reach consumers. In this their special applicability and their specific energy niche, which not busy while by any another PS. On land their role is similar nuclear submarines, which thanking NPS have got completely other quality of autonomy in the discharge of underwater kettles.The territory of Russia on the area both variety of resources and manufactures allows to implement different variants and combinations of

  8. Fast and efficient three-step target-specific curing of a virulence plasmid in Salmonella enterica.

    Science.gov (United States)

    de Moraes, Marcos H; Teplitski, Max

    2015-12-01

    Virulence plasmids borne by serovars of Salmonella enterica carry genes involved in its pathogenicity, as well as other functions. Characterization of phenotypes associated with virulence plasmids requires a system for efficiently curing strains of their virulence plasmids. Here, we developed a 3-step protocol for targeted curing of virulence plasmids. The protocol involves insertion of an I-SecI restriction site linked to an antibiotic resistance gene into the target plasmid using λ-Red mutagenesis, followed by the transformation with a temperature-sensitive auxiliary plasmid which carries I-SecI nuclease expressed from a tetracycline-inducible promoter. Finally, the auxiliary plasmid is removed by incubation at 42 °C and the plasmid-less strains are verified on antibiotic-containing media. This method is fast and very efficient: over 90 % of recovered colonies lacked their virulence plasmid.

  9. Antibody-drug conjugates: Promising and efficient tools for targeted cancer therapy.

    Science.gov (United States)

    Nasiri, Hadi; Valedkarimi, Zahra; Aghebati-Maleki, Leili; Majidi, Jafar

    2018-09-01

    Over the recent decades, the use of antibody-drug conjugates (ADCs) has led to a paradigm shift in cancer chemotherapy. Antibody-based treatment of various human tumors has presented dramatic efficacy and is now one of the most promising strategies used for targeted therapy of patients with a variety of malignancies, including hematological cancers and solid tumors. Monoclonal antibodies (mAbs) are able to selectively deliver cytotoxic drugs to tumor cells, which express specific antigens on their surface, and has been suggested as a novel category of agents for use in the development of anticancer targeted therapies. In contrast to conventional treatments that cause damage to healthy tissues, ADCs use mAbs to specifically attach to antigens on the surface of target cells and deliver their cytotoxic payloads. The therapeutic success of future ADCs depends on closely choosing the target antigen, increasing the potency of the cytotoxic cargo, improving the properties of the linker, and reducing drug resistance. If appropriate solutions are presented to address these issues, ADCs will play a more important role in the development of targeted therapeutics against cancer in the next years. We review the design of ADCs, and focus on how ADCs can be exploited to overcome multiple drug resistance (MDR). © 2018 Wiley Periodicals, Inc.

  10. New Drug Candidate Targeting the 4A1 Orphan Nuclear Receptor for Medullary Thyroid Cancer Therapy

    Directory of Open Access Journals (Sweden)

    Lei Zhang

    2018-03-01

    Full Text Available Medullary thyroid cancer (MTC is a relatively rare thyroid cancer responsible for a substantial fraction of thyroid cancer mortality. More effective therapeutic drugs with low toxicity for MTC are urgently needed. Orphan nuclear receptor 4A1 (NR4A1 plays a pivotal role in regulating the proliferation and apoptosis of a variety of tumor cells. Based on the NR4A1 protein structure, 2-imino-6-methoxy-2H-chromene-3-carbothioamide (IMCA was identified from the Specs compounds database using the protein structure-guided virtual screening approach. Computationally-based molecular modeling studies suggested that IMCA has a high affinity for the ligand binding pocket of NR4A1. MTT [3-(4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-H-tetrazolium bromide] and apoptosis assays demonstrated that IMCA resulted in significant thyroid cancer cell death. Immunofluorescence assays showed that IMCA induced NR4A1 translocation from the nucleus to the cytoplasm in thyroid cancer cell lines, which may be involved in the cell apoptotic process. In this study, the quantitative polymerase chain reaction results showed that the IMCA-induced upregulation of sestrin1 and sestrin2 was dose-dependent in thyroid cancer cell lines. Western blot showed that IMCA increased phosphorylation of adenosine 5′-monophosphate-activated protein kinase (AMPK and decreased phosphorylation of ribosomal protein S6 kinase (p70S6K, which is the key enzyme in the mammalian target of rapamycin (mTOR pathway. The experimental results suggest that IMCA is a drug candidate for MTC therapy and may work by increasing the nuclear export of NR4A1 to the cytoplasm and the tumor protein 53 (p53-sestrins-AMPK-mTOR signaling pathway.

  11. New Drug Candidate Targeting the 4A1 Orphan Nuclear Receptor for Medullary Thyroid Cancer Therapy.

    Science.gov (United States)

    Zhang, Lei; Liu, Wen; Wang, Qun; Li, Qinpei; Wang, Huijuan; Wang, Jun; Teng, Tieshan; Chen, Mingliang; Ji, Ailing; Li, Yanzhang

    2018-03-02

    Medullary thyroid cancer (MTC) is a relatively rare thyroid cancer responsible for a substantial fraction of thyroid cancer mortality. More effective therapeutic drugs with low toxicity for MTC are urgently needed. Orphan nuclear receptor 4A1 (NR4A1) plays a pivotal role in regulating the proliferation and apoptosis of a variety of tumor cells. Based on the NR4A1 protein structure, 2-imino-6-methoxy-2H-chromene-3-carbothioamide (IMCA) was identified from the Specs compounds database using the protein structure-guided virtual screening approach. Computationally-based molecular modeling studies suggested that IMCA has a high affinity for the ligand binding pocket of NR4A1. MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] and apoptosis assays demonstrated that IMCA resulted in significant thyroid cancer cell death. Immunofluorescence assays showed that IMCA induced NR4A1 translocation from the nucleus to the cytoplasm in thyroid cancer cell lines, which may be involved in the cell apoptotic process. In this study, the quantitative polymerase chain reaction results showed that the IMCA-induced upregulation of sestrin1 and sestrin2 was dose-dependent in thyroid cancer cell lines. Western blot showed that IMCA increased phosphorylation of adenosine 5'-monophosphate-activated protein kinase (AMPK) and decreased phosphorylation of ribosomal protein S6 kinase (p70S6K), which is the key enzyme in the mammalian target of rapamycin (mTOR) pathway. The experimental results suggest that IMCA is a drug candidate for MTC therapy and may work by increasing the nuclear export of NR4A1 to the cytoplasm and the tumor protein 53 (p53)-sestrins-AMPK-mTOR signaling pathway.

  12. Moab, Utah: Using Energy Data to Target Carbon Reductions from Building Energy Efficiency (City Energy: From Data to Decisions)

    Energy Technology Data Exchange (ETDEWEB)

    Strategic Priorities and Impact Analysis Team, Office of Strategic Programs

    2017-11-01

    This fact sheet "Moab, Utah: Using Energy Data to Target Carbon Reductions from Building Energy Efficiency" explains how the City of Moab used data from the U.S. Department of Energy's Cities Leading through Energy Analysis and Planning (Cities-LEAP) and the State and Local Energy Data (SLED) programs to inform its city energy planning. It is one of ten fact sheets in the "City Energy: From Data to Decisions" series.

  13. Somatic Cell Nuclear Transfer Followed by CRIPSR/Cas9 Microinjection Results in Highly Efficient Genome Editing in Cloned Pigs

    Directory of Open Access Journals (Sweden)

    Timothy P. Sheets

    2016-12-01

    Full Text Available The domestic pig is an ideal “dual purpose” animal model for agricultural and biomedical research. With the availability of genome editing tools such as clustered regularly interspaced short palindromic repeat (CRISPR and associated nuclease Cas9 (CRISPR/Cas9, it is now possible to perform site-specific alterations with relative ease, and will likely help realize the potential of this valuable model. In this article, we investigated for the first time a combination of somatic cell nuclear transfer (SCNT and direct injection of CRISPR/Cas ribonucleoprotein complex targeting GRB10 into the reconstituted oocytes to generate GRB10 ablated Ossabaw fetuses. This strategy resulted in highly efficient (100% generation of biallelic modifications in cloned fetuses. By combining SCNT with CRISPR/Cas9 microinjection, genome edited animals can now be produced without the need to manage a founder herd, while simultaneously eliminating the need for laborious in vitro culture and screening. Our approach utilizes standard cloning techniques while simultaneously performing genome editing in the cloned zygotes of a large animal model for agriculture and biomedical applications.

  14. Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus.

    Science.gov (United States)

    Merkle, Florian T; Neuhausser, Werner M; Santos, David; Valen, Eivind; Gagnon, James A; Maas, Kristi; Sandoe, Jackson; Schier, Alexander F; Eggan, Kevin

    2015-05-12

    The CRISPR-Cas9 system has the potential to revolutionize genome editing in human pluripotent stem cells (hPSCs), but its advantages and pitfalls are still poorly understood. We systematically tested the ability of CRISPR-Cas9 to mediate reporter gene knockin at 16 distinct genomic sites in hPSCs. We observed efficient gene targeting but found that targeted clones carried an unexpectedly high frequency of insertion and deletion (indel) mutations at both alleles of the targeted gene. These indels were induced by Cas9 nuclease, as well as Cas9-D10A single or dual nickases, and often disrupted gene function. To overcome this problem, we designed strategies to physically destroy or separate CRISPR target sites at the targeted allele and developed a bioinformatic pipeline to identify and eliminate clones harboring deleterious indels at the other allele. This two-pronged approach enables the reliable generation of knockin hPSC reporter cell lines free of unwanted mutations at the targeted locus. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  15. Efficient Nonlocal M-Control and N-Target Controlled Unitary Gate Using Non-symmetric GHZ States

    Science.gov (United States)

    Chen, Li-Bing; Lu, Hong

    2018-03-01

    Efficient local implementation of a nonlocal M-control and N-target controlled unitary gate is considered. We first show that with the assistance of two non-symmetric qubit(1)-qutrit(N) Greenberger-Horne-Zeilinger (GHZ) states, a nonlocal 2-control and N-target controlled unitary gate can be constructed from 2 local two-qubit CNOT gates, 2 N local two-qutrit conditional SWAP gates, N local qutrit-qubit controlled unitary gates, and 2 N single-qutrit gates. At each target node, the two third levels of the two GHZ target qutrits are used to expose one and only one initial computational state to the local qutrit-qubit controlled unitary gate, instead of being used to hide certain states from the conditional dynamics. This scheme can be generalized straightforwardly to implement a higher-order nonlocal M-control and N-target controlled unitary gate by using M non-symmetric qubit(1)-qutrit(N) GHZ states as quantum channels. Neither the number of the additional levels of each GHZ target particle nor that of single-qutrit gates needs to increase with M. For certain realistic physical systems, the total gate time may be reduced compared with that required in previous schemes.

  16. The effect of cojugate energy consumptions on comparative efficiency of alternatives of the development of nuclear power engineering

    International Nuclear Information System (INIS)

    Bubnov, V.P.; Bykov, A.I.; Egorushkin, S.V.

    1987-01-01

    The effect of control of conjugate energy consumptions on comparative efficiency of alternatives of the development of nuclear power engineering, including NPPs with thermal and fast reactors has been calculated. Economical-mathematical models of the system of nuclear power engineering including the subsystems of fuel utilization, fuel supply and conjugate industries, have been used. It has been shown, that with allowance for conjugate energy consumptions, the economic characteristics of nuclear power engineering sharply degradate when it develops only on the basis of thermal reactors. Putting the fast reactors into the system of nuclear power engineering has an important effect on reduction of conjugate energy consumptions and favors the increase of the efficiency of the system functioning during the considered time interval (60 years). Positive effects from putting breeders into operation become appreciable only on the latest estimated time intervals

  17. Production of nuclear fragments from the interactions of 24 GeV/c protons in a gold target

    CERN Document Server

    Herz, A J; O'Sullivan, D; Thompson, A

    1976-01-01

    Lexan polycarbonate track detectors have been used to determine the charge and energy spectra of nuclear fragments with Z>or=6 and with kinetic energies as low as approximately=1.0 MeV/nucleon emitted from a thin gold target bombarded with 24 GeV/c protons. (8 refs).

  18. Nuclear and nucleolar localization signals and their targeting function in phosphatidylinositol 4-kinase PI4K230

    International Nuclear Information System (INIS)

    Kakuk, Annamaria; Friedlaender, Elza; Vereb, Gyoergy; Lisboa, Duarte; Bagossi, Peter; Toth, Gabor; Gergely, Pal; Vereb, Gyoergy

    2008-01-01

    PI4K230, an isoform of phosphatidylinositol 4-kinase, known primarily as a cytoplasmic membrane-bound enzyme, was detected recently also in the nucleolus of several cells. Here we provide mechanistic insight on the targeting function of its putative nuclear localization signal (NLS) sequences using molecular modeling, digitonin-permeabilized HeLa cells and binding to various importins. The synthetic sequence 916 NFNHIHKRIRRVADKYLSG 934 comprising a putative monopartite NLS (NLS1), targeted covalently bound fluorescent BSA to the nucleoplasm via classical importin α/β mechanism employing importins α1 and α3 but not α5. This transport was inhibited by wheat germ agglutinin and GTPγS. The sequence 1414 SKKTNRGSQLHKYYMKRRTL 1433 , a putative bipartite NLS (NLS2) proved ineffective in nuclear targeting if conjugated to fluorescently labeled BSA. Nonetheless, NLS2 or either of its basic clusters directed to the nucleolus soybean trypsin inhibitor that can pass the nuclear pore complex passively; moreover, an expressed 58 kDa fragment of PI4K230 (AA1166-1667) comprising NLS2 was also imported into the nucleus by import factors of reticulocyte lysate or by importin α1/β or α3/β complexes and localized to the nucleolus. We conclude that the putative bipartite NLS itself is a nucleolar targeting signal, and for nuclear import PI4K230 requires a larger sequence around it or, alternatively, the monopartite NLS

  19. An Efficient Method for Identifying Gene Fusions by Targeted RNA Sequencing from Fresh Frozen and FFPE Samples.

    Directory of Open Access Journals (Sweden)

    Jonathan A Scolnick

    Full Text Available Fusion genes are known to be key drivers of tumor growth in several types of cancer. Traditionally, detecting fusion genes has been a difficult task based on fluorescent in situ hybridization to detect chromosomal abnormalities. More recently, RNA sequencing has enabled an increased pace of fusion gene identification. However, RNA-Seq is inefficient for the identification of fusion genes due to the high number of sequencing reads needed to detect the small number of fusion transcripts present in cells of interest. Here we describe a method, Single Primer Enrichment Technology (SPET, for targeted RNA sequencing that is customizable to any target genes, is simple to use, and efficiently detects gene fusions. Using SPET to target 5701 exons of 401 known cancer fusion genes for sequencing, we were able to identify known and previously unreported gene fusions from both fresh-frozen and formalin-fixed paraffin-embedded (FFPE tissue RNA in both normal tissue and cancer cells.

  20. Locating sensors for detecting source-to-target patterns of special nuclear material smuggling: a spatial information theoretic approach.

    Science.gov (United States)

    Przybyla, Jay; Taylor, Jeffrey; Zhou, Xuesong

    2010-01-01

    In this paper, a spatial information-theoretic model is proposed to locate sensors for detecting source-to-target patterns of special nuclear material (SNM) smuggling. In order to ship the nuclear materials from a source location with SNM production to a target city, the smugglers must employ global and domestic logistics systems. This paper focuses on locating a limited set of fixed and mobile radiation sensors in a transportation network, with the intent to maximize the expected information gain and minimize the estimation error for the subsequent nuclear material detection stage. A Kalman filtering-based framework is adapted to assist the decision-maker in quantifying the network-wide information gain and SNM flow estimation accuracy.

  1. Locating Sensors for Detecting Source-to-Target Patterns of Special Nuclear Material Smuggling: A Spatial Information Theoretic Approach

    Directory of Open Access Journals (Sweden)

    Xuesong Zhou

    2010-08-01

    Full Text Available In this paper, a spatial information-theoretic model is proposed to locate sensors for detecting source-to-target patterns of special nuclear material (SNM smuggling. In order to ship the nuclear materials from a source location with SNM production to a target city, the smugglers must employ global and domestic logistics systems. This paper focuses on locating a limited set of fixed and mobile radiation sensors in a transportation network, with the intent to maximize the expected information gain and minimize the estimation error for the subsequent nuclear material detection stage. A Kalman filtering-based framework is adapted to assist the decision-maker in quantifying the network-wide information gain and SNM flow estimation accuracy.

  2. Efficient VEGF targeting delivery of DOX using Bevacizumab conjugated SiO2@LDH for anti-neuroblastoma therapy.

    Science.gov (United States)

    Zhu, Rongrong; Wang, Zhaoqi; Liang, Peng; He, Xiaolie; Zhuang, Xizhen; Huang, Ruiqi; Wang, Mei; Wang, Qigang; Qian, Yechang; Wang, Shilong

    2017-11-01

    Vascular endothelial growth factor (VEGF) plays an important role in angiogenesis and is highly expressed in carcinoma, which make it an important target for tumor targeting therapy. Neuroblastoma is the main cause for cancer-related death in children. Like most solid tumors, it is also accompanied with the overexpression of VEGF. Doxorubicin Hydrochloride (DOX), a typical chemotherapeutic agent, exhibits efficient anticancer activities for various cancers. However, DOX, without targeting ability, usually causes severe damage to normal tissues. To overcome the shortages, we designed a novel nano-composite, which is Bevacizumab (Bev) modified SiO 2 @LDH nanoparticles (SiO 2 @LDH-Bev), loading with DOX to achieve targeting ability and curative efficiency. SiO 2 @LDH-DOX and SiO 2 @LDH-Bev-DOX nanoparticles were synthesized and the physicochemical properties were characterized by TEM detection, Zeta potential analysis, FTIR, Raman and XPS analysis. Then in vitro and in vivo anti-neuroblastoma efficiency, targeting ability and mechanisms of anti-carcinoma and anti-angiogenesis of SiO 2 @LDH-Bev-DOX were explored. Our results indicated that we obtained the core-shell structure SiO 2 @LDH-Bev with an average diameter of 253±10nm and the amount of conjugated Bev was 4.59±0.38μg/mg SiO 2 @LDH-Bev. SiO 2 @LDH-Bev-DOX could improve the cellular uptake and the targeting effect of DOX to brain and tumor, enhance the anti-neuroblastoma and anti-angiogenesis efficiency both in vitro and in vivo, and alleviate side effects of DOX sharply, especially hepatic injury. In addition, we also demonstrated that angiogenesis inhibitory effect was mediated by DOX and VEGF triggered signal pathways, including PI3K/Akt, Raf/MEK/ERK, and adhesion related pathways. In summary, SiO 2 @LDH-Bev could be a potential VEGF targeting nanocarrier applied in VEGF positive cancer therapy. This paper explored that a novel core-shell structure nanomaterial SiO 2 @LDH and modified SiO 2 @LDH with

  3. Efficient intracellular delivery of 5-fluorodeoxyuridine into colon cancer cells by targeted immunoliposomes

    NARCIS (Netherlands)

    Koning, GA; Kamps, JAAM; Scherphof, GL

    2002-01-01

    Immunoliposomes, liposomes with monoclonal antibodies attached, are being developed for targeting the anti-cancer drug 5-fluoro-2'-deoxyuridine (FUdR) to colon cancer cells. A monoclonal antibody against the rat colon carcinoma CC531 was covalently coupled to liposomes containing a dipalmitoylated

  4. Efficient committed budget for implementing target audit probability for many inspectees

    NARCIS (Netherlands)

    Yim, A.T.L.

    2009-01-01

    Strategic models of auditor-inspectee interaction have neglected implementation details in multiple-inspectee settings. With multiple inspectees, the target audit probability derived from the standard analysis can be implemented with sampling plans differing in the budgets committed to support them.

  5. Target Pilots Energy Efficiency Measures for Broad Rollout in Existing Stores

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-03-01

    Target Corporation partnered with the U.S. Department of Energy (DOE) to develop and implement solutions to retrofit existing buildings to reduce annual energy consumption by at least 30% versus requirements set by ASHRAE/ANSI/IESNA Standard 90.1-20041 as part of DOE’s Commercial Building Partnership (CBP) program.

  6. Enhancement of the efficiency of magnetic targeting for drug delivery: Development and evaluation of magnet system

    International Nuclear Information System (INIS)

    Cao Quanliang; Han Xiaotao; Li Liang

    2011-01-01

    Deep magnetic capture and clinical application are the current trends for magnetic targeted drug delivery system. More promising and possible strategies are needed to overcome the current limitations and further improve the magnetic targeting technique. Recent advances in the development of targeting magnet system show promise in progressing this technology from the laboratory to the clinic. Starting from well-known basic concepts, current limitations of magnetic targeted drug delivery system are analyzed. Meanwhile, the design concepts and evaluations of some effective improvements in magnet system are discussed and reviewed with reference to (i) reasonable design of magnet system; (ii) control modes of magnet system used to generate dynamical magnetic fields; and (iii) magnetic field driving types. - Research Highlights: → The current limitations of MTDDS for deep capture and clinical application are analyzed. → The development of magnet system shows promise in progressing MTDDS to clinical application. → The design concepts and evaluations of improvements in magnet system are reviewed and discussed. → The key to improve magnet system lies in controllable magnets and different excitations.

  7. An efficient numerical target strength prediction model: Validation against analysis solutions

    NARCIS (Netherlands)

    Fillinger, L.; Nijhof, M.J.J.; Jong, C.A.F. de

    2014-01-01

    A decade ago, TNO developed RASP (Rapid Acoustic Signature Prediction), a numerical model for the prediction of the target strength of immersed underwater objects. The model is based on Kirchhoff diffraction theory. It is currently being improved to model refraction, angle dependent reflection and

  8. Computational model for a high temperature electrolyzer coupled to a HTTR for efficient nuclear hydrogen production

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Daniel; Rojas, Leorlen; Rosales, Jesus; Castro, Landy; Gamez, Abel; Brayner, Carlos, E-mail: danielgonro@gmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil); Garcia, Lazaro; Garcia, Carlos; Torre, Raciel de la, E-mail: lgarcia@instec.cu [Instituto Superior de Tecnologias y Ciencias Aplicadas (InSTEC), La Habana (Cuba); Sanchez, Danny [Universidade Estadual de Santa Cruz (UESC), Ilheus, BA (Brazil)

    2015-07-01

    High temperature electrolysis process coupled to a very high temperature reactor (VHTR) is one of the most promising methods for hydrogen production using a nuclear reactor as the primary heat source. However there are not references in the scientific publications of a test facility that allow to evaluate the efficiency of the process and other physical parameters that has to be taken into consideration for its accurate application in the hydrogen economy as a massive production method. For this lack of experimental facilities, mathematical models are one of the most used tools to study this process and theirs flowsheets, in which the electrolyzer is the most important component because of its complexity and importance in the process. A computational fluid dynamic (CFD) model for the evaluation and optimization of the electrolyzer of a high temperature electrolysis hydrogen production process flowsheet was developed using ANSYS FLUENT®. Electrolyzer's operational and design parameters will be optimized in order to obtain the maximum hydrogen production and the higher efficiency in the module. This optimized model of the electrolyzer will be incorporated to a chemical process simulation (CPS) code to study the overall high temperature flowsheet coupled to a high temperature accelerator driven system (ADS) that offers advantages in the transmutation of the spent fuel. (author)

  9. High efficiency-large capacity circulating water pump for Hamaoka Nuclear Power Station unit No.3

    International Nuclear Information System (INIS)

    Ito, Akihiko; Sasamuro, Takemi; Takeda, Hirohisa.

    1988-01-01

    No.3 plant in the Hamaoka Nuclear Power Station, Chube Electric Power Co., Inc. is the latest plant of 1100 MW class BWR type, which began the commercial operation in August, 1987. The seawater intake and discharge system of this plant is composed of the channel exceeding 2 km in the total length from the intake tower to the discharge port. The circulating water pump installed in this system has the capacity of 1620 m 3 /min and the total head of 16.5 m, which are the largest in the world. It attained the efficiency as high as more than 90%. Three pumps supply seawater to three-body condensers. The design of the impeller and the casing for obtaining high efficiency, the structural design for facilitating maintenance, the manufacture of a model pump and the performance test using it and so on are reported. The most important item in the manufacture was the form of the onebody impeller weighing 4.5t. The confirmation of the performance of the actual machines was carried out as a part of the synthetic function confirmation test at the power station, and the flow rate was measured with Pitot tubes and ultrasonic flowmeters. (Kako, I.)

  10. Computational model for a high temperature electrolyzer coupled to a HTTR for efficient nuclear hydrogen production

    International Nuclear Information System (INIS)

    Gonzalez, Daniel; Rojas, Leorlen; Rosales, Jesus; Castro, Landy; Gamez, Abel; Brayner, Carlos; Garcia, Lazaro; Garcia, Carlos; Torre, Raciel de la; Sanchez, Danny

    2015-01-01

    High temperature electrolysis process coupled to a very high temperature reactor (VHTR) is one of the most promising methods for hydrogen production using a nuclear reactor as the primary heat source. However there are not references in the scientific publications of a test facility that allow to evaluate the efficiency of the process and other physical parameters that has to be taken into consideration for its accurate application in the hydrogen economy as a massive production method. For this lack of experimental facilities, mathematical models are one of the most used tools to study this process and theirs flowsheets, in which the electrolyzer is the most important component because of its complexity and importance in the process. A computational fluid dynamic (CFD) model for the evaluation and optimization of the electrolyzer of a high temperature electrolysis hydrogen production process flowsheet was developed using ANSYS FLUENT®. Electrolyzer's operational and design parameters will be optimized in order to obtain the maximum hydrogen production and the higher efficiency in the module. This optimized model of the electrolyzer will be incorporated to a chemical process simulation (CPS) code to study the overall high temperature flowsheet coupled to a high temperature accelerator driven system (ADS) that offers advantages in the transmutation of the spent fuel. (author)

  11. Hydrothermal modeling for the efficient design of thermal loading in a nuclear waste repository

    International Nuclear Information System (INIS)

    Cho, Won-Jin; Kim, Jin-Seop; Choi, Heui-Joo

    2014-01-01

    Highlights: • Three-dimensional hydrothermal modeling for HLW repository is performed. • The model reduces the peak temperature in the repository by about 10 °C. • Decreasing the tunnel distance is more efficient to improve the disposal density. • The EDZ surrounding the deposition hole increases the peak temperature. • The peak temperature for the double-layer repository remains below the limit. - Abstract: The thermal analysis of a geological repository for nuclear waste using the three-dimensional hydrothermal model is performed. The hydrothermal model reduces the maximum peak temperature in the repository by about 10 °C compared to the heat conduction model with constant thermal conductivities. Decreasing the tunnel distance is more efficient than decreasing the deposition hole spacing to improve the disposal density for a given thermal load. The annular excavation damaged zone surrounding the deposition hole has a considerable effect on the peak temperature. The possibility of double-layer repository is analyzed from the viewpoint of the thermal constraints of the repository. The maximum peak temperature for the double-layer repository is slightly higher than that for the single-layer repository, but remains below the temperature limit

  12. Efficient methods for targeted mutagenesis in zebrafish using zinc-finger nucleases: data from targeting of nine genes using CompoZr or CoDA ZFNs.

    Directory of Open Access Journals (Sweden)

    Raman Sood

    Full Text Available Recently, it has been shown that targeted mutagenesis using zinc-finger nucleases (ZFNs and transcription activator-like effector nucleases (TALENs can be used to generate knockout zebrafish lines for analysis of their function and/or developing disease models. A number of different methods have been developed for the design and assembly of gene-specific ZFNs and TALENs, making them easily available to most zebrafish researchers. Regardless of the choice of targeting nuclease, the process of generating mutant fish is similar. It is a time-consuming and multi-step process that can benefit significantly from development of efficient high throughput methods. In this study, we used ZFNs assembled through either the CompoZr (Sigma-Aldrich or the CoDA (context-dependent assembly platforms to generate mutant zebrafish for nine genes. We report our improved high throughput methods for 1 evaluation of ZFNs activity by somatic lesion analysis using colony PCR, eliminating the need for plasmid DNA extractions from a large number of clones, and 2 a sensitive founder screening strategy using fluorescent PCR with PIG-tailed primers that eliminates the stutter bands and accurately identifies even single nucleotide insertions and deletions. Using these protocols, we have generated multiple mutant alleles for seven genes, five of which were targeted with CompoZr ZFNs and two with CoDA ZFNs. Our data also revealed that at least five-fold higher mRNA dose was required to achieve mutagenesis with CoDA ZFNs than with CompoZr ZFNs, and their somatic lesion frequency was lower (<5% when compared to CopmoZr ZFNs (9-98%. This work provides high throughput protocols for efficient generation of zebrafish mutants using ZFNs and TALENs.

  13. An efficient Neuro-Fuzzy approach to nuclear power plant transient identification

    Energy Technology Data Exchange (ETDEWEB)

    Gomes da Costa, Rafael [Instituto de Engenharia Nuclear - CNEN, Programa de Pos-Graduacao em Ciencia e Tecnologia Nucleares, Via Cinco, s/no, Cidade Universitaria, Rua Helio de Almeida, 75, Postal Box 68550, Zip Code 21941-906 Rio de Janeiro (Brazil); Abreu Mol, Antonio Carlos de, E-mail: mol@ien.gov.br [Instituto de Engenharia Nuclear - CNEN, Programa de Pos-Graduacao em Ciencia e Tecnologia Nucleares, Via Cinco, s/no, Cidade Universitaria, Rua Helio de Almeida, 75, Postal Box 68550, Zip Code 21941-906 Rio de Janeiro (Brazil); Instituto Nacional de C and T de Reatores Nucleares Inovadores (Brazil); Carvalho, Paulo Victor R. de, E-mail: paulov@ien.gov.br [Instituto de Engenharia Nuclear - CNEN, Programa de Pos-Graduacao em Ciencia e Tecnologia Nucleares, Via Cinco, s/no, Cidade Universitaria, Rua Helio de Almeida, 75, Postal Box 68550, Zip Code 21941-906 Rio de Janeiro (Brazil); Lapa, Celso Marcelo Franklin, E-mail: lapa@ien.gov.br [Instituto de Engenharia Nuclear - CNEN, Programa de Pos-Graduacao em Ciencia e Tecnologia Nucleares, Via Cinco, s/no, Cidade Universitaria, Rua Helio de Almeida, 75, Postal Box 68550, Zip Code 21941-906 Rio de Janeiro (Brazil); Instituto Nacional de C and T de Reatores Nucleares Inovadores (Brazil)

    2011-06-15

    Highlights: > We investigate a Neuro-Fuzzy modeling tool use for able transient identification. > The prelusive transient type identification is done by an artificial neural network. > After, the fuzzy-logic system analyzes the results emitting reliability degree of it. > The research support was made in a PWR simulator at the Brazilian Nuclear Engineering Institute. > The results show the potential to help operators' decisions in a nuclear power plant. - Abstract: Transient identification in nuclear power plants (NPP) is often a computational very hard task and may involve a great amount of human cognition. The early identification of unexpected departures from steady state behavior is an essential step for the operation, control and accident management in NPPs. The bases for the transient identification relay on the evidence that different system faults and anomalies lead to different pattern evolution in the involved process variables. During an abnormal event, the operator must monitor a great amount of information from the instruments that represents a specific type of event. Recently, several works have been developed for transient identification. These works frequently present a non reliable response, using the 'don't know' as the system output. In this work, we investigate the possibility of using a Neuro-Fuzzy modeling tool for efficient transient identification, aiming to helping the operator crew to take decisions relative to the procedure to be followed in situations of accidents/transients at NPPs. The proposed system uses artificial neural networks (ANN) as first level transient diagnostic. After the ANN has done the preliminary transient type identification, a fuzzy-logic system analyzes the results emitting reliability degree of it. A validation of this identification system was made at the three loops Pressurized Water Reactor (PWR) simulator of the Human-System Interface Laboratory (LABIHS) of the Nuclear Engineering Institute

  14. An efficient Neuro-Fuzzy approach to nuclear power plant transient identification

    International Nuclear Information System (INIS)

    Gomes da Costa, Rafael; Abreu Mol, Antonio Carlos de; Carvalho, Paulo Victor R. de; Lapa, Celso Marcelo Franklin

    2011-01-01

    Highlights: → We investigate a Neuro-Fuzzy modeling tool use for able transient identification. → The prelusive transient type identification is done by an artificial neural network. → After, the fuzzy-logic system analyzes the results emitting reliability degree of it. → The research support was made in a PWR simulator at the Brazilian Nuclear Engineering Institute. → The results show the potential to help operators' decisions in a nuclear power plant. - Abstract: Transient identification in nuclear power plants (NPP) is often a computational very hard task and may involve a great amount of human cognition. The early identification of unexpected departures from steady state behavior is an essential step for the operation, control and accident management in NPPs. The bases for the transient identification relay on the evidence that different system faults and anomalies lead to different pattern evolution in the involved process variables. During an abnormal event, the operator must monitor a great amount of information from the instruments that represents a specific type of event. Recently, several works have been developed for transient identification. These works frequently present a non reliable response, using the 'don't know' as the system output. In this work, we investigate the possibility of using a Neuro-Fuzzy modeling tool for efficient transient identification, aiming to helping the operator crew to take decisions relative to the procedure to be followed in situations of accidents/transients at NPPs. The proposed system uses artificial neural networks (ANN) as first level transient diagnostic. After the ANN has done the preliminary transient type identification, a fuzzy-logic system analyzes the results emitting reliability degree of it. A validation of this identification system was made at the three loops Pressurized Water Reactor (PWR) simulator of the Human-System Interface Laboratory (LABIHS) of the Nuclear Engineering Institute (IEN

  15. Human Cytomegalovirus Nuclear Egress Proteins Ectopically Expressed in the Heterologous Environment of Plant Cells are Strictly Targeted to the Nuclear Envelope.

    Science.gov (United States)

    Lamm, Christian E; Link, Katrin; Wagner, Sabrina; Milbradt, Jens; Marschall, Manfred; Sonnewald, Uwe

    2016-03-10

    In all eukaryotic cells, the nucleus forms a prominent cellular compartment containing the cell's nuclear genome. Although structurally similar, animal and plant nuclei differ substantially in details of their architecture. One example is the nuclear lamina, a layer of tightly interconnected filament proteins (lamins) underlying the nuclear envelope of metazoans. So far no orthologous lamin genes could be detected in plant genomes and putative lamin-like proteins are only poorly described in plants. To probe for potentially conserved features of metazoan and plant nuclear envelopes, we ectopically expressed the core nuclear egress proteins of human cytomegalovirus pUL50 and pUL53 in plant cells. pUL50 localizes to the inner envelope of metazoan nuclei and recruits the nuclear localized pUL53 to it, forming heterodimers. Upon expression in plant cells, a very similar localization pattern of both proteins could be determined. Notably, pUL50 is specifically targeted to the plant nuclear envelope in a rim-like fashion, a location to which coexpressed pUL53 becomes strictly corecruited from its initial nucleoplasmic distribution. Using pUL50 as bait in a yeast two-hybrid screening, the cytoplasmic re-initiation supporting protein RISP could be identified. Interaction of pUL50 and RISP could be confirmed by coexpression and coimmunoprecipitation in mammalian cells and by confocal laser scanning microscopy in plant cells, demonstrating partial pUL50-RISP colocalization in areas of the nuclear rim and other intracellular compartments. Thus, our study provides strong evidence for conserved structural features of plant and metazoan nuclear envelops and identifies RISP as a potential pUL50-interacting plant protein.

  16. Dissemination of National Nuclear-HRD Network for Efficient and Effective N-HRD for NPP-Embarking Countries

    International Nuclear Information System (INIS)

    Yamashita, K.; Toba, A.; Hirose, H.; Ikuta, Y.; Sawai, T.; Takahashi, A.; Ueda, K.; Kita, T.

    2016-01-01

    Full text: Close mutual cooperation among nuclear-related organizations, such as government, industry and academia is extremely useful to promote nuclear human resources development (HRD). National HRD network has already been established in Japan since Nov. 2010. The network has promoted the following five discussions: 1) elementary to high school education, 2) nuclear education at universities and colleges, 3) HRD for working engineers, 4) HRD to internationalize national human resources, and 5) supportive HRD activities to newly NPP introducing countries successfully. Through the establishment of the network, the communication has been strongly improved so that the Japan–IAEA joint Nuclear Energy Management School can be held successfully every year. Based on the good experience with the network, Japan would like to recommend the introduction of national nuclear HRD (N-HRD)-network to the NPP-embarking countries. We are interested in cooperation with IAEA for establishment of national N-HRD network for efficient and effective N-HRD. (author

  17. miR-370 suppresses HBV gene expression and replication by targeting nuclear factor IA.

    Science.gov (United States)

    Fan, Hongxia; Lv, Ping; Lv, Jing; Zhao, Xiaopei; Liu, Min; Zhang, Guangling; Tang, Hua

    2017-05-01

    Hepatitis B virus (HBV) infection is a major health problem worldwide. The roles of microRNAs in the regulation of HBV expression are being increasingly recognized. In this study, we found that overexpression of miR-370 suppressed HBV gene expression and replication in Huh7 cells, whereas antisense knockdown of endogenous miR-370 enhanced HBV gene expression and replication in Huh7 cells and HepG2.2.15 cells. Further, we identified the transcription factor nuclear factor IA (NFIA) as a new host target of miR-370. Overexpression and knockdown studies showed that NFIA stimulated HBV gene expression and replication. Importantly, overexpression of NFIA counteracted the effect of miR-370 on HBV gene expression and replication. Further mechanistic studies showed that miR-370 suppressed HBV replication and gene expression by repressing HBV Enhancer I activity, and one of the NFIA binding site in the Enhancer I element was responsible for the repressive effect of miR-370 on HBV Enhancer I activity. Altogether, our results demonstrated that miR-370 suppressed HBV gene expression and replication through repressing NFIA expression, which stimulates HBV replication via direct regulation on HBV Enhancer I activities. Our findings may provide a new antiviral strategy for HBV infection. J. Med. Virol. 89:834-844, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  18. Circumvention of nuclear factor kappaB-induced chemoresistance by cytoplasmic-targeted anthracyclines.

    Science.gov (United States)

    Bilyeu, Jennifer D; Panta, Ganesh R; Cavin, Lakita G; Barrett, Christina M; Turner, Eddie J; Sweatman, Trevor W; Israel, Mervyn; Lothstein, Leonard; Arsura, Marcello

    2004-04-01

    Nuclear factor kappaB (NF-kappaB) has been implicated in inducible chemoresistance against anthracyclines. In an effort to improve the cytotoxicity of anthracyclines while reducing their cardiotoxic effects, we have developed a novel class of extranuclear-localizing 14-O-acylanthracyclines that bind to the phorbol ester/diacylglycerol-binding C1b domain of conventional and novel protein kinase C (PKC) isoforms, thereby promoting an apoptotic response. Because PKCs have been shown to be involved in NF-kappaB activation, in this report, we determined the mechanism of NF-kappaB activation by N-benzyladriamycin-14-valerate (AD 198) and N-benzyladriamycin-14-pivalate (AD 445), two novel 14-O-acylanthracylines. We show that the induction of NF-kappaB activity in response to drug treatment relies on the activation of PKC-delta and NF-kappaB-activating kinase (NAK), independent of ataxia telengectasia mutated and p53 activities. In turn, NAK activates the IKK complex through phosphorylation of the IKK-2 subunit. We find that neither NF-kappaB activation nor ectopic expression of Bcl-X(L) confers protection from AD 198-induced cell killing. Overall, our data indicate that activation of novel PKC isoforms by cytoplasmic-targeted 14-O-acylanthracyclines promotes an apoptotic response independent of DNA damage, which is unimpeded by inducible activation of NF-kappaB.

  19. Target values for nuclear material safeguards measurements - motivation or burden to operators?

    International Nuclear Information System (INIS)

    Weh, R.; Kuhn, K.D.

    1989-01-01

    The analytical determination of material streams and inventories plays an important part in those nuclear facilities called bulk-handling facilities in safeguards terminology. Reprocessing plants and mixed-oxide fabrication facilities are typical examples. With respect to their safeguards, the relevant regulations attach fundamental importance to material accountancy. The balance itself is examined by International Atomic Energy Agency (IAEA) inspectors and within the boundaries of the European Communities by Euratom inspectors as well, with regard to formal correctness. The analytical methods of accountancy in, for example, reprocessing plants, make high demands on the qualifications of the analyst. A conscientious analyst will, of course, try to fulfill his task as well and effectively as possible. These target values will become a burden, however, when they have been drawn up for purely scientific interest and the operator has been urged to achieve them on the pretext of improving safeguards. There are basically two reasons for which the authors have misgivings in this respect. First, the measurement system, which the material balance is based on, has to conform to the latest international regulations. This could easily lead to a permanent obligation of updating for the plants concerned. Second, the goal quantities set by the IAEA will induce an attempt to adjust measurement techniques and chemical analysis to goals totally unsuitable for large-scale plants

  20. Forward production of charged pions with incident protons on nuclear targets at the CERN Proton Synchrotron

    CERN Document Server

    Apollonio, M.; Bagulya, A.; Barr, G.; Blondel, A.; Bobisut, F.; Bogomilov, M.; Bonesini, M.; Booth, C.; Borghi, S.; Bunyatov, S.; Burguet-Castell, J.; Catanesi, M.G.; Cervera-Villanueva, A.; Chimenti, P.; Coney, L.; Di Capua, E.; Dore, U.; Dumarchez, J.; Edgecock, R.; Ellis, M.; Ferri, F.; Gastaldi, U.; Giani, S.; Giannini, G.; Gibin, D.; Gilardoni, S.; Gorbunov, P.; Gossling, C.; Gomez-Cadenas, J.J.; Grant, A.; Graulich, J.S.; Gregoire, G.; Grichine, V.; Grossheim, A.; Guglielmi, A.; Howlett, L.; Ivanchenko, A.; Ivanchenko, V.; Kayis-Topaksu, A.; Kirsanov, M.; Kolev, D.; Krasnoperov, A.; Martin-Albo, J.; Meurer, C.; Mezzetto, M.; Mills, G.B.; Morone, M.C.; Novella, P.; Orestano, D.; Palladino, V.; Panman, J.; Papadopoulos, I.; Pastore, F.; Piperov, Stefan; Polukhina, N.; Popov, B.; Prior, G.; Radicioni, E.; Schmitz, D.; Schroeter, R.; Serdiouk, V.; Skoro, G; Sorel, M.; Tcherniaev, E.; Temnikov, P.; Tereschenko, V.; Tonazzo, A.; Tortora, L.; Tsenov, R.; Tsukerman, I.; Vidal-Sitjes, G.; Wiebusch, C.; Zucchelli, P.

    2009-01-01

    Measurements of the double-differential charged pion production cross-section in the range of momentum 0.5 GeV/c < p < 8.0 GeV/c and angle 0.025 rad < theta <0.25 rad in collisions of protons on beryllium, carbon, nitrogen, oxygen, aluminium, copper, tin, tantalum and lead are presented. The data were taken with the large acceptance HARP detector in the T9 beam line of the CERN PS. Incident particles were identified by an elaborate system of beam detectors. The data were taken with thin targets of 5% of a nuclear interaction length. The tracking and identification of the produced particles was performed using the forward system of the HARP experiment. Results are obtained for the double-differential cross section mainly at four incident proton beam momenta (3 GeV/c, 5 GeV/c, 8 GeV/c and 12 GeV/c). Measurements are compared with the GEANT4 and MARS Monte Carlo generators. A global parametrization is provided as an approximation of all the collected datasets which can serve as a tool for quick yield...

  1. How to Design a Targeted Agricultural Subsidy System: Efficiency or Equity?

    DEFF Research Database (Denmark)

    Cong, Ronggang; Brady, Mark

    2012-01-01

    for environmental protection, social cohesion, etc. Based on a simplified economic model, we prove that there is “reverse redistribution” in the current tax-subsidy system, which cannot be avoided. To find a possible way to distribute subsidies more efficiently and equitably, several alternative subsidy systems...... (the pure loan, the harvest tax and the income contingent loan) are presented and examined....

  2. Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection

    Directory of Open Access Journals (Sweden)

    Marius Rutkauskas

    2015-03-01

    Full Text Available CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against foreign nucleic acids. In type I CRISPR-Cas systems, invading DNA is detected by a large ribonucleoprotein surveillance complex called Cascade. The crRNA component of Cascade is used to recognize target sites in foreign DNA (protospacers by formation of an R-loop driven by base-pairing complementarity. Using single-molecule supercoiling experiments with near base-pair resolution, we probe here the mechanism of R-loop formation and detect short-lived R-loop intermediates on off-target sites bearing single mismatches. We show that R-loops propagate directionally starting from the protospacer-adjacent motif (PAM. Upon reaching a mismatch, R-loop propagation stalls and collapses in a length-dependent manner. This unambiguously demonstrates that directional zipping of the R-loop accomplishes efficient target recognition by rapidly rejecting binding to off-target sites with PAM-proximal mutations. R-loops that reach the protospacer end become locked to license DNA degradation by the auxiliary Cas3 nuclease/helicase without further target verification.

  3. Effect of linkers on the αvβ3 integrin targeting efficiency of cyclic RGD-conjugates

    Science.gov (United States)

    Karmakar, Partha; Grabowska, Dorota; Sudlow, Gail; Ziabrev, Kostiantyn; Sanyal, Nibedita; Achilefu, Samuel

    2018-02-01

    Cyclic arginine-glycine-aspartic acid (cRGD) peptides are well known to target ανβ3 integrin expressed on cancer cells and neovasculature. Conjugation of these peptides with dyes, drugs, antibodies and other biomolecules through covalent linkers provides a facile way to deliver these products to tumor cells for targeted cancer therapy and diagnosis. Click chemistry and acid-amine couplings are widely used conjugation strategies. However, the effects of different linkers and the distance between the cRGD and the conjugates on the binding of cRGD ligand with ανβ3 has been underexplored. In this present study, we prepared cRGD-conjugates using different linkers and determined how they altered the tumor targeting efficiency in vitro and in vivo. The results demonstrate that different linkers significantly altered the pharmacokinetics of the cRGD conjugates and the tumor uptake kinetics. Unlike large antibodies, this preliminary finding shows that linkers used to attach drugs and fluorescent molecular probes to small peptides play a major role in the accuracy of tumor targeting and treatment outcomes. As a result, considerable attention should be paid to the nature of linkers used in the design of molecular probes and targeted therapeutics.

  4. External tandem target system for efficient production of short-lived positron emitting radionuclides

    International Nuclear Information System (INIS)

    Koh, K.; Dwyer, J.; Finn, R.; Sheh, Y.; Sinnreich, J.; Wooten, T.

    1983-01-01

    Recent developments in radiopharmaceutical chemistry allow the incorporation of short-lived, positron-emitting radionuclides into a variety of compounds which when used with a positron emission tomograph provide a means of monitoring physiological disorders by a standard technique. To effectively meet the increased ''in-house'' clinical demands while maintaining a production schedule, a tandem target was designed and has been installed for the simultaneous ''on-line'' preparation of oxygen-15 labelled compounds such as CO 2 15 , H 2 O 15 ; and nitrogen-13 labelled compounds such as 13 NH 3 , 13 N 2 O, and 13 N 2 . The processing time required for the synthesis of the nitrogen-13 products as compared to the essentially instantaneous formation of oxygen-15 labelled compounds has provided the necessary time delay for clinical utilization. The characterisitcs of this external tandem target system as well as the automation for the dual processing are presented

  5. Formulation and Characterization of Pyrazinamide Polymeric Nanoparticles for Pulmonary Tuberculosis: Efficiency for Alveolar Macrophage Targeting

    OpenAIRE

    Varma, J. N. Ravi; Kumar, T. Santosh; Prasanthi, B.; Ratna, J. Vijaya

    2015-01-01

    Pyrazinamide, a highly specific agent against Mycobacterium tuberculosis is used as first-line drug to treat tuberculosis. The current work aims to formulate polymeric nanoparticles based drug delivery system to sustain the release profile and reduce the dosing frequency of pyrazinamide. Further aim was to target the macrophages within body fluid. These polymeric nanoparticles were prepared by simultaneous double-emulsion (W/O/W) solvent evaporation/diffusion technique. The prepared dispersio...

  6. Novel uses of a wide beam saddle field ion source for producing targets used in nuclear physics experiments at the Argonne National Laboratory ATLAS facility

    International Nuclear Information System (INIS)

    Greene, J.P.; Thomas, G.E.

    1996-01-01

    The wide beam ion sputter source has several unique characteristics which make it very useful for producing, reducing the thickness or cleaning the surface of targets needed for nuclear physics experiments. A discussion of these techniques as well as the sputter source characteristics will be given. Sputter yields obtained utilizing the source are presented for a variety of materials common to nuclear target production

  7. Efficient neutron production from sub-nanosecond laser pulse accelerating deuterons on target front side

    Czech Academy of Sciences Publication Activity Database

    Klír, Daniel; Krása, Josef; Cikhardt, Jakub; Dudžák, Roman; Krouský, Eduard; Pfeifer, Miroslav; Řezáč, Karel; Sila, O.; Skála, Jiří; Ullschmied, Jiří; Velyhan, Andriy

    2015-01-01

    Roč. 22, č. 9 (2015), s. 093117-093117 ISSN 1070-664X R&D Projects: GA ČR GAP205/12/0454; GA MŠk(CZ) LG13029; GA MŠk LM2010014; GA MŠk EE2.3.20.0279 Grant - others:LaserZdroj (OP VK 3)(XE) CZ.1.07/2.3.00/20.0279 Institutional support: RVO:61389021 ; RVO:68378271 Keywords : plasma * femtosecond laser * nuclear-fusion * ion-beams * hot-electrons Subject RIV: BL - Plasma and Gas Discharge Physics; BL - Plasma and Gas Discharge Physics (FZU-D) OBOR OECD: Fluids and plasma physics (including surface physics); Fluids and plasma physics (including surface physics) (FZU-D) Impact factor: 2.207, year: 2015 http://scitation.aip.org/content/aip/journal/pop/22/9/10.1063/1.4931460

  8. The role of non-elastic nuclear processes for intermediate-energy protons in silicon targets

    Energy Technology Data Exchange (ETDEWEB)

    Hormaza, Joel Mesa, E-mail: jmesa@ibb.unesp.br [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil); Garcia, Cesar E., E-mail: cgarcia@instec.cu [Instituto Superior de Tecnologias y Ciencias Aplicadas (InSTEC), Havana (Cuba); Arruda Neto, Joao D.T.; Rodrigues, Tulio E., E-mail: arruda@if.usp.br, E-mail: tulio@if.usp.br [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Instituto de Fisica; Schelin, Hugo R.; Denyak, Valery, E-mail: schelin@utfpr.edu.br, E-mail: denyak@gmail.com [Instituto de Pesquisa Pele Pequeno Principe, Curitiba, PR (Brazil); Paschuck, Sergei A.; Evseev, Ivan, E-mail: sergei@utfpr.edu.br, E-mail: evseev@utfpr.edu.br [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil)

    2013-07-01

    The transportation of energetic ions in bulk matter is of direct interest in several areas including shielding against ions originating from either space radiations or terrestrial accelerators, cosmic ray propagation studies in galactic medium, or radiobiological effects resulting from the work place or clinical exposures. For carcinogenesis, terrestrial radiation therapy, and radiobiological research, knowledge of beam composition and interactions is necessary to properly evaluate the effects on human and animal tissues. For the proper assessment of radiation exposures both reliable transport codes and accurate input parameters are needed. In the last years efforts have been increasing in order to develop more effective models to describe and predict the damages induced by radiation in electronic devices. In this sense, the interaction of protons with those devices, particularly which operate in space, is a topic of paramount importance, mainly because although the majority of them are made with silicon, experimental data on p+Si nuclear processes is very sparse. In this work we have used a new quite sophisticated Monte Carlo multicollisional intranuclear cascade (MCMC) code for pre-equilibrium emission, plus de-excitation of residual nucleus by two ways: evaporation of particles (mainly nucleons, but also composites) and possibly fragmentation/fission in the case of heavy residues, in order to study some observable of nuclear interaction of protons between 100-200 MeV in a {sup 28}Si target. The code has been developed with very recent improvements that take into account Pauli blocking effects in a novel and more precise way, as well as a more rigorous energy balance, an energy stopping time criterion for pre-equilibrium emission and the inclusion of deuteron, triton and 3He emissions in the evaporation step, which eventually concurs with fragmentation/break-up stage. The fragment mass distributions, as well as the multiplicities and the spectra of secondary

  9. The role of non-elastic nuclear processes for intermediate-energy protons in silicon targets

    International Nuclear Information System (INIS)

    Hormaza, Joel Mesa; Garcia, Cesar E.; Arruda Neto, Joao D.T.; Rodrigues, Tulio E.; Paschuck, Sergei A.; Evseev, Ivan

    2013-01-01

    The transportation of energetic ions in bulk matter is of direct interest in several areas including shielding against ions originating from either space radiations or terrestrial accelerators, cosmic ray propagation studies in galactic medium, or radiobiological effects resulting from the work place or clinical exposures. For carcinogenesis, terrestrial radiation therapy, and radiobiological research, knowledge of beam composition and interactions is necessary to properly evaluate the effects on human and animal tissues. For the proper assessment of radiation exposures both reliable transport codes and accurate input parameters are needed. In the last years efforts have been increasing in order to develop more effective models to describe and predict the damages induced by radiation in electronic devices. In this sense, the interaction of protons with those devices, particularly which operate in space, is a topic of paramount importance, mainly because although the majority of them are made with silicon, experimental data on p+Si nuclear processes is very sparse. In this work we have used a new quite sophisticated Monte Carlo multicollisional intranuclear cascade (MCMC) code for pre-equilibrium emission, plus de-excitation of residual nucleus by two ways: evaporation of particles (mainly nucleons, but also composites) and possibly fragmentation/fission in the case of heavy residues, in order to study some observable of nuclear interaction of protons between 100-200 MeV in a 28 Si target. The code has been developed with very recent improvements that take into account Pauli blocking effects in a novel and more precise way, as well as a more rigorous energy balance, an energy stopping time criterion for pre-equilibrium emission and the inclusion of deuteron, triton and 3He emissions in the evaporation step, which eventually concurs with fragmentation/break-up stage. The fragment mass distributions, as well as the multiplicities and the spectra of secondary particles

  10. Orally Targeted Delivery of Tripeptide KPV via Hyaluronic Acid-Functionalized Nanoparticles Efficiently Alleviates Ulcerative Colitis.

    Science.gov (United States)

    Xiao, Bo; Xu, Zhigang; Viennois, Emilie; Zhang, Yuchen; Zhang, Zhan; Zhang, Mingzhen; Han, Moon Kwon; Kang, Yuejun; Merlin, Didier

    2017-07-05

    Overcoming adverse effects and selectively delivering drug to target cells are two major challenges in the treatment of ulcerative colitis (UC). Lysine-proline-valine (KPV), a naturally occurring tripeptide, has been shown to attenuate the inflammatory responses of colonic cells. Here, we loaded KPV into hyaluronic acid (HA)-functionalized polymeric nanoparticles (NPs). The resultant HA-KPV-NPs had a desirable particle size (∼272.3 nm) and a slightly negative zeta potential (∼-5.3 mV). These NPs successfully mediated the targeted delivery of KPV to key UC therapy-related cells (colonic epithelial cells and macrophages). In addition, these KPV-loaded NPs appear to be nontoxic and biocompatible with intestinal cells. Intriguingly, we found that HA-KPV-NPs exert combined effects against UC by both accelerating mucosal healing and alleviating inflammation. Oral administration of HA-KPV-NPs encapsulated in a hydrogel (chitosan/alginate) exhibited a much stronger capacity to prevent mucosa damage and downregulate TNF-α, thus they showed a much better therapeutic efficacy against UC in a mouse model, compared with a KPV-NP/hydrogel system. These results collectively demonstrate that our HA-KPV-NP/hydrogel system has the capacity to release HA-KPV-NPs in the colonic lumen and that these NPs subsequently penetrate into colitis tissues and enable KPV to be internalized into target cells, thereby alleviating UC. Copyright © 2016 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.

  11. Badoca Safari Park : a proposition on how to efficiently target the young segment

    OpenAIRE

    Almeida, Francisca Simões de

    2016-01-01

    Badoca Safari Park is an Animal Theme Park, which provides the opportunity to spend a day in contact with nature and different animal species. The Safari Park, pioneer in Portugal, achieved successful performances during the first years of activity. However, annual attendance has been declining over the last years across all age groups. The current study focuses on the 15-24 years old target, who are of strategic relevance, given the Park’s poor performance in retaining the young sement as th...

  12. Efficiency mark of the two-product power complex of nuclear power plant

    Science.gov (United States)

    Khrustalev, V. A.; Suchkov, V. M.

    2017-11-01

    The article discusses the combining nuclear power plants (NPP) with pressurized water reactors and distillation-desalination plants (DDP), their joint mode of operation during periods of coating failures of the electric power load graphs and thermo-economical efficiency. Along with the release of heat and generation of electric energy a desalination complex with the nuclear power plant produces distillate. Part of the selected steam “irretrievably lost” with a mix of condensation of this vapor in a desalination machine with a flow of water for distillation. It means that this steam transforms into condition of acquired product - distillate. The article presents technical solutions for the return of the working fluid for turbine К-1000-60/1500-2 и К-1200-6,8/50, as well as permissible part of low pressure regime according to the number of desalination units for each turbine. Patent for the proposed two-product energy complex, obtained by Gagarin State Technical University is analyzed. The energy complex has such system advantages as increasing the capacity factor of a nuclear reactor and also allows to solve the problem of shortage of fresh water. Thermo-economics effectiveness of this complex is determined by introducing a factor-“thermo-economic index”. During analyzing of the results of the calculations of a thermo-economic index we can see a strong influence of the cost factor of the distillate on the market. Then higher participation of the desalination plant in coverage of the failures of the graphs of the electric loading then smaller the payback period of the NPP. It is manifested more clearly, as it’s shown in the article, when pricing options depend on time of day and the configuration of the daily electric load diagram. In the geographical locations of the NPPs with PWR the Russian performance in a number of regions with low freshwater resources and weak internal electrical connections combined with DDP might be one of the ways to improve the

  13. Target Plate Material Influence on Fullerene-C60 Laser Desorption/Ionization Efficiency

    Science.gov (United States)

    Zeegers, Guido P.; Günthardt, Barbara F.; Zenobi, Renato

    2016-04-01

    Systematic laser desorption/ionization (LDI) experiments of fullerene-C60 on a wide range of target plate materials were conducted to gain insight into the initial ion formation in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The positive and negative ion signal intensities of precursor, fragment, and cluster ions were monitored, varying both the laser fluence (0-3.53 Jcm-2) and the ion extraction delay time (0-950 ns). The resulting species-specific ion signal intensities are an indication for the ionization mechanisms that contribute to LDI and the time frames in which they operate, providing insight in the (MA)LDI primary ionization. An increasing electrical resistivity of the target plate material increases the fullerene-C60 precursor and fragment anion signal intensity. Inconel 625 and Ti90/Al6/V4, both highly electrically resistive, provide the highest anion signal intensities, exceeding the cation signal intensity by a factor ~1.4 for the latter. We present a mechanism based on transient electrical field strength reduction to explain this trend. Fullerene-C60 cluster anion formation is negligible, which could be due to the high extraction potential. Cluster cations, however, are readily formed, although for high laser fluences, the preferred channel is formation of precursor and fragment cations. Ion signal intensity depends greatly on the choice of substrate material, and careful substrate selection could, therefore, allow for more sensitive (MA)LDI measurements.

  14. Clathrin to Lipid Raft-Endocytosis via Controlled Surface Chemistry and Efficient Perinuclear Targeting of Nanoparticle.

    Science.gov (United States)

    Chakraborty, Atanu; Jana, Nikhil R

    2015-09-17

    Nanoparticle interacts with live cells depending on their surface chemistry, enters into cell via endocytosis, and is commonly trafficked to an endosome/lysozome that restricts subcellular targeting options. Here we show that nanoparticle surface chemistry can be tuned to alter their cell uptake mechanism and subcellular trafficking. Quantum dot based nanoprobes of 20-30 nm hydrodynamic diameters have been synthesized with tunable surface charge (between +15 mV to -25 mV) and lipophilicity to influence their cellular uptake processes and subcellular trafficking. It is observed that cationic nanoprobe electrostatically interacts with cell membrane and enters into cell via clathrin-mediated endocytosis. At lower surface charge (between +10 mV to -10 mV), the electrostatic interaction with cell membrane becomes weaker, and additional lipid raft endocytosis is initiated. If a lipophilic functional group is introduced on a weakly anionic nanoparticle surface, the uptake mechanism shifts to predominant lipid raft-mediated endocytosis. In particular, the zwitterionic-lipophilic nanoprobe has the unique advantage as it weakly interacts with anionic cell membrane, migrates toward lipid rafts for interaction through lipophilic functional group, and induces lipid raft-mediated endocytosis. While predominate or partial clathrin-mediated entry traffics most of the nanoprobes to lysozome, predominate lipid raft-mediated entry traffics them to perinuclear region, particularly to the Golgi apparatus. This finding would guide in designing appropriate nanoprobe for subcellular targeting and delivery.

  15. Open Access Target Validation Is a More Efficient Way to Accelerate Drug Discovery

    Science.gov (United States)

    Lee, Wen Hwa

    2015-01-01

    There is a scarcity of novel treatments to address many unmet medical needs. Industry and academia are finally coming to terms with the fact that the prevalent models and incentives for innovation in early stage drug discovery are failing to promote progress quickly enough. Here we will examine how an open model of precompetitive public–private research partnership is enabling efficient derisking and acceleration in the early stages of drug discovery, whilst also widening the range of communities participating in the process, such as patient and disease foundations. PMID:26042736

  16. How to design a targeted agricultural subsidy system: efficiency or equity?

    Science.gov (United States)

    Cong, Rong-Gang; Brady, Mark

    2012-01-01

    In this paper we appraise current agricultural subsidy policy in the EU. Several sources of its inefficiency are identified: it is inefficient for supporting farmers' incomes or guaranteeing food security, and irrational transfer payments decoupled from actual performance that may be negative for environmental protection, social cohesion, etc. Based on a simplified economic model, we prove that there is "reverse redistribution" in the current tax-subsidy system, which cannot be avoided. To find a possible way to distribute subsidies more efficiently and equitably, several alternative subsidy systems (the pure loan, the harvest tax and the income contingent loan) are presented and examined.

  17. How to design a targeted agricultural subsidy system: efficiency or equity?

    Directory of Open Access Journals (Sweden)

    Rong-Gang Cong

    Full Text Available In this paper we appraise current agricultural subsidy policy in the EU. Several sources of its inefficiency are identified: it is inefficient for supporting farmers' incomes or guaranteeing food security, and irrational transfer payments decoupled from actual performance that may be negative for environmental protection, social cohesion, etc. Based on a simplified economic model, we prove that there is "reverse redistribution" in the current tax-subsidy system, which cannot be avoided. To find a possible way to distribute subsidies more efficiently and equitably, several alternative subsidy systems (the pure loan, the harvest tax and the income contingent loan are presented and examined.

  18. Undercovering the hidden links. Nuclear and isotope techniques target nutritional needs

    International Nuclear Information System (INIS)

    Iyengar, Venkatesh

    2001-01-01

    Global nutrition problems raise a host of questions and warrant action by the international community of scientists, nutritionists, physicians and other medical professionals. What steps should be taken to remedy this situation? How can this be accomplished economically? How can progress be monitored? What is the role of technology in the overall monitoring process? The last question, which is most relevant to this article, is of particular importance to the IAEA and its support of nutrition programmes. The IAEA's activities in human nutrition were initiated to apply nuclear and related isotopic techniques for solving problems prevalent in developing countries. Among the numerous applications available, isotopic techniques are uniquely well suited to targeting and tracking progress in food and nutrition development programmes. These are tools that help evaluate nutritional status of individuals and populations, measure nutrient requirements and the uptake and bio-availability of vitamins and minerals. The IAEA's efforts help to: verify the nature of the nutrition problem and the efficacy of specific interventions; implement nutrition intervention programmes by monitoring effectiveness and reducing programme costs; guide in the processing of local foods for optimal nutritional value; serve as early indicators of important long-term health improvements; and strengthen capacity building in developing countries. Among the numerous applications available, isotopic techniques are uniquely well suited to targeting and tracking progress in food and nutrition development programmes. These are tools that help evaluate nutritional status of individuals and populations, measure nutrient requirements and the uptake and bio-availability of vitamins and minerals. The IAEA's efforts help to: verify the nature of the nutrition problem and the efficacy of specific interventions; implement nutrition intervention programmes by monitoring effectiveness and reducing programme costs

  19. Anti-EGFR-iRGD recombinant protein conjugated silk fibroin nanoparticles for enhanced tumor targeting and antitumor efficiency

    Directory of Open Access Journals (Sweden)

    Bian X

    2016-05-01

    Full Text Available Xinyu Bian,* Puyuan Wu,* Huizi Sha, Hanqing Qian, Qing Wang, Lei Cheng, Yang Yang, Mi Yang, Baorui LiuComprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University, Clinical Cancer Institute of Nanjing University, Nanjing, People’s Republic of China*These authors contributed equally to this workAbstract: In this study, we report a novel kind of targeting with paclitaxel (PTX-loaded silk fibroin nanoparticles conjugated with iRGD–EGFR nanobody recombinant protein (anti-EGFR-iRGD. The new nanoparticles (called A-PTX-SF-NPs were prepared using the carbodiimide-mediated coupling procedure and their characteristics were evaluated. The cellular cytotoxicity and cellular uptake of A-PTX-SF-NPs were also investigated. The results in vivo suggested that NPs conjugated with the recombinant protein exhibited more targeting and anti-neoplastic property in cells with high EGFR expression. In the in vivo antitumor efficacy assay, the A-PTX-SF-NPs group showed slower tumor growth and smaller tumor volumes than PTX-SF-NPs in a HeLa xenograft mouse model. A real-time near-infrared fluorescence imaging study showed that A-PTX-SF-NPs could target the tumor more effectively. These results suggest that the anticancer activity and tumor targeting of A-PTX-SF-NPs were superior to those of PTX-SF-NPs and may have the potential to be used for targeted delivery for tumor therapies. Keywords: EGFR, nanobody, iRGD, recombinant protein, targeting drug carriers, antitumor efficiency

  20. Impact of target mRNA structure on siRNA silencing efficiency: A large-scale study.

    Science.gov (United States)

    Gredell, Joseph A; Berger, Angela K; Walton, S Patrick

    2008-07-01

    The selection of active siRNAs is generally based on identifying siRNAs with certain sequence and structural properties. However, the efficiency of RNA interference has also been shown to depend on the structure of the target mRNA, primarily through studies using exogenous transcripts with well-defined secondary structures in the vicinity of the target sequence. While these studies provide a means for examining the impact of target sequence and structure independently, the predicted secondary structures for these transcripts are often not reflective of structures that form in full-length, native mRNAs where interactions can occur between relatively remote segments of the mRNAs. Here, using a combination of experimental results and analysis of a large dataset, we demonstrate that the accessibility of certain local target structures on the mRNA is an important determinant in the gene silencing ability of siRNAs. siRNAs targeting the enhanced green fluorescent protein were chosen using a minimal siRNA selection algorithm followed by classification based on the predicted minimum free energy structures of the target transcripts. Transfection into HeLa and HepG2 cells revealed that siRNAs targeting regions of the mRNA predicted to have unpaired 5'- and 3'-ends resulted in greater gene silencing than regions predicted to have other types of secondary structure. These results were confirmed by analysis of gene silencing data from previously published siRNAs, which showed that mRNA target regions unpaired at either the 5'-end or 3'-end were silenced, on average, approximately 10% more strongly than target regions unpaired in the center or primarily paired throughout. We found this effect to be independent of the structure of the siRNA guide strand. Taken together, these results suggest minimal requirements for nucleation of hybridization between the siRNA guide strand and mRNA and that both mRNA and guide strand structure should be considered when choosing candidate si

  1. Proton induced target fragmentation studies on solid state nuclear track detectors using Carbon radiators

    Science.gov (United States)

    Szabó, J.; Pálfalvi, J. K.; Strádi, A.; Bilski, P.; Swakoń, J.; Stolarczyk, L.

    2018-04-01

    One of the limiting factors of an astronaut's career is the dose received from space radiation. High energy protons, being the main components of the complex radiation field present on a spacecraft, give a significant contribution to the dose. To investigate the behavior of solid state nuclear track detectors (SSNTDs) if they are irradiated by such particles, SSNTD stacks containing carbon blocks were exposed to high energy proton beams (70, 100, 150 and 230 MeV) at the Proteus cyclotron, IFJ PAN -Krakow. The incident protons cannot be detected directly; however, tracks of secondary particles, recoils and fragments of the constituent atoms of the detector material and of the carbon radiator are formed. It was found that as the proton energy increases, the number of tracks induced in the PADC material by secondary particles decreases. From the measured geometrical parameters of the tracks the linear energy transfer (LET) spectrum and the dosimetric quantities were determined, applying appropriate calibration. In the LET spectra the LET range of the most important secondary particles could be identified and their abundance showed differences in the spectra if the detectors were short or long etched. The LET spectra obtained on the SSNTDs irradiated by protons were compared to LET spectra of detectors flown on the International Space Station (ISS): they were quite similar, resulting in a quality factor difference of only 5%. Thermoluminescent detectors (TLDs) were applied in each case to measure the dose from primary protons and other lower LET particles present in space. Comparing and analyzing the results of the TLD and SSNTD measurements, it was obtained that proton induced target fragments contributed to the total absorbed dose in 3.2% and to the dose equivalent in 14.2% in this particular space experiment.

  2. Efficient photoreceptor-targeted gene expression in vivo by recombinant adeno-associated virus.

    Science.gov (United States)

    Flannery, J G; Zolotukhin, S; Vaquero, M I; LaVail, M M; Muzyczka, N; Hauswirth, W W

    1997-06-24

    We describe a general approach for achieving efficient and cell type-specific expression of exogenous genes in photoreceptor cells of the mammalian retina. Recombinant adeno-associated virus (rAAV) vectors were used to transfer the bacterial lacZ gene or a synthetic green fluorescent protein gene (gfp) to mouse or rat retinas after injection into the subretinal space. Using a proximal murine rod opsin promoter (+86 to -385) to drive expression, reporter gene product was found exclusively in photoreceptors, not in any other retinal cell type or in the adjacent retinal pigment epithelium. GFP-expressing photoreceptors typically encompassed 10-20% of the total retinal area after a single 2-microl injection. Photoreceptors were transduced with nearly 100% efficiency in the region directly surrounding the injection site. We estimate approximately 2.5 million photoreceptors were transduced as a result of the single subretinal inoculation. This level of gene transfer and expression suggests the feasibility of genetic therapy for retinal disease. The gfp-containing rAAV stock was substantially free of both adenovirus and wild-type AAV, as judged by plaque assay and infectious center assay, respectively. Thus, highly purified, helper virus-free rAAV vectors can achieve high-frequency tissue-specific transduction of terminally differentiated, postmitotic photoreceptor cells.

  3. Implementing targeted region capture sequencing for the clinical detection of Alagille syndrome: An efficient and cost‑effective method.

    Science.gov (United States)

    Huang, Tianhong; Yang, Guilin; Dang, Xiao; Ao, Feijian; Li, Jiankang; He, Yizhou; Tang, Qiyuan; He, Qing

    2017-11-01

    Alagille syndrome (AGS) is a highly variable, autosomal dominant disease that affects multiple structures including the liver, heart, eyes, bones and face. Targeted region capture sequencing focuses on a panel of known pathogenic genes and provides a rapid, cost‑effective and accurate method for molecular diagnosis. In a Chinese family, this method was used on the proband and Sanger sequencing was applied to validate the candidate mutation. A de novo heterozygous mutation (c.3254_3255insT p.Leu1085PhefsX24) of the jagged 1 gene was identified as the potential disease‑causing gene mutation. In conclusion, the present study suggested that target region capture sequencing is an efficient, reliable and accurate approach for the clinical diagnosis of AGS. Furthermore, these results expand on the understanding of the pathogenesis of AGS.

  4. Nanoparticle-neural stem cells for targeted ovarian cancer treatment: optimization of silica nanoparticles for efficient drug loading

    Science.gov (United States)

    Patel, Z.; Berlin, J.; Abidi, W.

    2018-02-01

    One of the drugs used to treat ovarian cancer is cisplatin. However, cisplatin kills normal surrounding tissue in addition to cancer cells. To improve tumor targeting efficiency, our lab uses neural stem cells (NSCs), which migrate directly to ovarian tumors. If free cisplatin is loaded into NSCs for targeted drug delivery, it will kill the NSCs. To prevent the drug cisplatin from killing both the NSCs and normal surrounding tissue, our lab synthesizes silica nanoparticles (SiNPs) that act as a protective carrier. The big picture here is to maximize efficiency of tumor targeting using NSCs and minimize toxicity to these NSCs using SiNPs. The goal of this project is to optimize the stability of SiNPs, which is important for efficient drug loading. To do this, the concentration of tetraethyl orthosilicate (TEOS), one of the main components of SiNPs, was varied. We hypothesized that more TEOS equates to more stable SiNPs because TEOS contributes carbon to SiNPs, and thus a tightly-packed chemical structure results in a stable particle. Then, the stability of the SiNPs were checked in cell media and phosphate buffered saline (PBS). Lastly, the SiNPs were analyzed for their porosity using the transmission electron microscope (TEM). TEM imaging showed white spots in the 200-800 μL TEOS batches and no white spots in the 1000-1800 μL TEOS batches. The white spots were pores, which indicate instability. We concluded that the ultimate factor that determines the stability of SiNPs (100 nm) is the concentration of organic substance.

  5. Multiple cues on the physiochemical, mesenchymal, and intracellular trafficking interactions with nanocarriers to maximize tumor target efficiency

    Directory of Open Access Journals (Sweden)

    Kim SW

    2015-06-01

    Full Text Available Sang-Woo Kim, Dongwoo Khang Nanomedicine Laboratory, Department of Molecular Medicine, School of Medicine, Gachon University, Incheon, South Korea Abstract: Over the past 60 years, numerous medical strategies have been employed to overcome neoplasms. In fact, with the exception of lung, bronchial, and pancreatic cancers, the 5-year survival rate of most cancers currently exceeds 70%. However, the quality of life of patients during chemotherapy remains unsatisfactory despite the increase in survival rate. The side effects of current chemotherapies stem from poor target efficiency at tumor sites due to the uncontrolled biodistribution of anticancer agents (ie, conventional or current approved nanodrugs. This review discusses the effective physiochemical factors for determining biodistribution of nanocarriers and, ultimately, increasing tumor-targeting probability by avoiding the reticuloendothelial system. Second, stem cell-conjugated nanotherapeutics was addressed to maximize the tumor searching ability and to inhibit tumor growth. Lastly, physicochemical material properties of anticancer nanodrugs were discussed for targeting cellular organelles with modulation of drug-release time. A better understanding of suggested topics will increase the tumor-targeting ability of anticancer drugs and, ultimately, promote the quality of life of cancer patients during chemotherapy. Keywords: cancer, anticancer nanodrugs, mesenchymal stem cell, intracellular trafficking

  6. Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

    Directory of Open Access Journals (Sweden)

    Lazar Andreas

    2008-02-01

    Full Text Available Abstract Background In real-time PCR, it is necessary to consider the efficiency of amplification (EA of amplicons in order to determine initial target levels properly. EAs can be deduced from standard curves, but these involve extra effort and cost and may yield invalid EAs. Alternatively, EA can be extracted from individual fluorescence curves. Unfortunately, this is not reliable enough. Results Here we introduce simultaneous non-linear fitting to determine – without standard curves – an optimal common EA for all samples of a group. In order to adjust EA as a function of target fluorescence, and still to describe fluorescence as a function of cycle number, we use an iterative algorithm that increases fluorescence cycle by cycle and thus simulates the PCR process. A Gauss peak function is used to model the decrease of EA with increasing amplicon accumulation. Our approach was validated experimentally with hydrolysis probe or SYBR green detection with dilution series of 5 different targets. It performed distinctly better in terms of accuracy than standard curve, DART-PCR, and LinRegPCR approaches. Based on reliable EAs, it was possible to detect that for some amplicons, extraordinary fluorescence (EA > 2.00 was generated with locked nucleic acid hydrolysis probes, but not with SYBR green. Conclusion In comparison to previously reported approaches that are based on the separate analysis of each curve and on modelling EA as a function of cycle number, our approach yields more accurate and precise estimates of relative initial target levels.

  7. DDR: Efficient computational method to predict drug–target interactions using graph mining and machine learning approaches

    KAUST Repository

    Olayan, Rawan S.

    2017-11-23

    Motivation Finding computationally drug-target interactions (DTIs) is a convenient strategy to identify new DTIs at low cost with reasonable accuracy. However, the current DTI prediction methods suffer the high false positive prediction rate. Results We developed DDR, a novel method that improves the DTI prediction accuracy. DDR is based on the use of a heterogeneous graph that contains known DTIs with multiple similarities between drugs and multiple similarities between target proteins. DDR applies non-linear similarity fusion method to combine different similarities. Before fusion, DDR performs a pre-processing step where a subset of similarities is selected in a heuristic process to obtain an optimized combination of similarities. Then, DDR applies a random forest model using different graph-based features extracted from the DTI heterogeneous graph. Using five repeats of 10-fold cross-validation, three testing setups, and the weighted average of area under the precision-recall curve (AUPR) scores, we show that DDR significantly reduces the AUPR score error relative to the next best start-of-the-art method for predicting DTIs by 34% when the drugs are new, by 23% when targets are new, and by 34% when the drugs and the targets are known but not all DTIs between them are not known. Using independent sources of evidence, we verify as correct 22 out of the top 25 DDR novel predictions. This suggests that DDR can be used as an efficient method to identify correct DTIs.

  8. Lipoprotein-biomimetic nanostructure enables efficient targeting delivery of siRNA to Ras-activated glioblastoma cells via macropinocytosis

    Science.gov (United States)

    Huang, Jia-Lin; Jiang, Gan; Song, Qing-Xiang; Gu, Xiao; Hu, Meng; Wang, Xiao-Lin; Song, Hua-Hua; Chen, Le-Pei; Lin, Ying-Ying; Jiang, Di; Chen, Jun; Feng, Jun-Feng; Qiu, Yong-Ming; Jiang, Ji-Yao; Jiang, Xin-Guo; Chen, Hong-Zhuan; Gao, Xiao-Ling

    2017-05-01

    Hyperactivated Ras regulates many oncogenic pathways in several malignant human cancers including glioblastoma and it is an attractive target for cancer therapies. Ras activation in cancer cells drives protein internalization via macropinocytosis as a key nutrient-gaining process. By utilizing this unique endocytosis pathway, here we create a biologically inspired nanostructure that can induce cancer cells to `drink drugs' for targeting activating transcription factor-5 (ATF5), an overexpressed anti-apoptotic transcription factor in glioblastoma. Apolipoprotein E3-reconstituted high-density lipoprotein is used to encapsulate the siRNA-loaded calcium phosphate core and facilitate it to penetrate the blood-brain barrier, thus targeting the glioblastoma cells in a macropinocytosis-dependent manner. The nanostructure carrying ATF5 siRNA exerts remarkable RNA-interfering efficiency, increases glioblastoma cell apoptosis and inhibits tumour cell growth both in vitro and in xenograft tumour models. This strategy of targeting the macropinocytosis caused by Ras activation provides a nanoparticle-based approach for precision therapy in glioblastoma and other Ras-activated cancers.

  9. WAYS TO IMPROVE THE EFFICIENCY OF THE PROGAM-TARGETED OF INFRASTRUCTURE OBJECTS IN RUSSIA

    Directory of Open Access Journals (Sweden)

    A. V. Frolova

    2014-01-01

    Full Text Available The relevance of this article due to the fact that the current situation inRussiais complemented by the negative eff ects of market reforms in the economy, initiated economic sanctions that have been taken against our country. In view of this, for the eff ective fi nancing costs of the federal budget for the development of transport infrastructure is a very topical issues related to the targeted program planning. The study aims to study ways to improve the effi ciency of program fi nancing expenditures of the federal budget for the development of transport infrastructure. The results will contribute to solving urgent problems in the development of transport infrastructure, high technology and housing sectors.

  10. Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

    Czech Academy of Sciences Publication Activity Database

    Vaněček, Václav; Zablotskyy, Vitaliy A.; Forostyak, Serhiy; Růžička, Jiří; Herynek, V.; Babič, Michal; Jendelová, Pavla; Kubinová, Šárka; Dejneka, Alexandr; Syková, Eva

    2012-01-01

    Roč. 7, 16 Jul (2012), s. 3719-3730 E-ISSN 1178-2013 R&D Projects: GA ČR(CZ) GAP304/12/1370; GA ČR GAP304/11/0731; GA ČR(CZ) GAP304/11/0189; GA ČR GAP304/11/0653; GA AV ČR IAA500390902 Institutional research plan: CEZ:AV0Z50390703; CEZ:AV0Z10100522; CEZ:AV0Z40500505 Keywords : nanoparticles * mesenchymal stem cells * magnetic targeting Subject RIV: FH - Neurology; BM - Solid Matter Physics ; Magnetism (FZU-D); FH - Neurology (UMCH-V) Impact factor: 3.463, year: 2012

  11. Polynomial algorithms for the Maximal Pairing Problem: efficient phylogenetic targeting on arbitrary trees

    Directory of Open Access Journals (Sweden)

    Stadler Peter F

    2010-06-01

    Full Text Available Abstract Background The Maximal Pairing Problem (MPP is the prototype of a class of combinatorial optimization problems that are of considerable interest in bioinformatics: Given an arbitrary phylogenetic tree T and weights ωxy for the paths between any two pairs of leaves (x, y, what is the collection of edge-disjoint paths between pairs of leaves that maximizes the total weight? Special cases of the MPP for binary trees and equal weights have been described previously; algorithms to solve the general MPP are still missing, however. Results We describe a relatively simple dynamic programming algorithm for the special case of binary trees. We then show that the general case of multifurcating trees can be treated by interleaving solutions to certain auxiliary Maximum Weighted Matching problems with an extension of this dynamic programming approach, resulting in an overall polynomial-time solution of complexity (n4 log n w.r.t. the number n of leaves. The source code of a C implementation can be obtained under the GNU Public License from http://www.bioinf.uni-leipzig.de/Software/Targeting. For binary trees, we furthermore discuss several constrained variants of the MPP as well as a partition function approach to the probabilistic version of the MPP. Conclusions The algorithms introduced here make it possible to solve the MPP also for large trees with high-degree vertices. This has practical relevance in the field of comparative phylogenetics and, for example, in the context of phylogenetic targeting, i.e., data collection with resource limitations.

  12. Hierarchical mesosilicalite nanoformulation integrated with cisplatin exhibits target-specific efficient anticancer activity

    Science.gov (United States)

    Jermy, B. Rabindran; Acharya, Sadananda; Ravinayagam, Vijaya; Alghamdi, Hajer Saleh; Akhtar, Sultan; Basuwaidan, Rehab S.

    2018-04-01

    Hierarchically structured zeolitic ZSM-5 and meso MCM-41 interlinked domain had an impeccable use as catalysis in many applications. The aim of the study was to develop a new drug delivery nanoformulation, specifically, cisplatin/mesosilicalite using top-down approach for cancer therapy. Hierarchical mesosilicalite with variable porosity was synthesized using alkaline molar solution (0.2 and 0.7 M NaOH) and was loaded with cisplatin through equilibrium adsorption technique. Physico-chemical properties of the nanoformulation (IAUM-56—Imam Abdulrahman Bin Faisal University Mesosilicalite-56) were characterized using X-ray diffraction, surface area analysis (BET), Fourier transformed infrared spectroscopy (FT-IR), diffuse reflectance UV-Vis spectroscopy, and transmission electron microscopy. Drug release study and anticancer activity were assayed on HeLa and MCF7 cancer cells using MTT assay. X-ray diffraction pattern showed interrelated meso- and microphases, while BET analysis revealed considerable mesoporosity formation with a remodulation of isotherm hysteresis indicating the presence of hierarchical pores. FT-IR showed the presence of nanozeolitic subunits into mesostructure with a band at about 550 cm-1. IAUM-56 demonstrated high cytotoxic activity against HeLa cancer cells with an LC50 of 0.02 mg/ml, MCF7 cancer cells with an LC50 of 0.05 mg/ml, and less toxic to normal fibroblast cells with an LC50 of approximately ten times higher at 0.5 mg/ml. Overall, IAUM-56 showed a high rate of sustained release of cisplatin imparting target specific cytotoxic effect against tumor cells with at least tenfold lower toxicity on normal fibroblast cells. Our nanoformulation has the potential use in cancer therapy as a targeted drug delivery system.

  13. The efficiency of the use of penetration nuclear logging in hydrogeology and engineering geology

    International Nuclear Information System (INIS)

    Ferronsky, V.I.

    1992-01-01

    The latest developments in equipment and techniques for nuclear and combined non-nuclear logging in friable unconsolidated deposits, including marine bottom sediments are described. The effectiveness of these techniques in hydrogeological and engineering geological investigations is discussed. (Author)

  14. Regeneration of the iodine isotope-exchange efficiency for nuclear-grade activated carbons

    International Nuclear Information System (INIS)

    Deitz, V.R.

    1985-01-01

    The removal of radioactive iodine from air flows passing through impregnated activated carbons depends on a minimum of three distinguishable reactions: (1) adsorption on the carbon networks of the activated carbons, (2) iodine isotope exchange with impregnated iodine-127, and (3) chemical combination with impregnated tertiary amines when present. When a carbon is new, all three mechanisms are at peak performance and it is not possible to distinguish among the three reactions by a single measurement; the retention of methyl iodide-127 is usually equal to the retention of methyl iodide-131. After the carbon is placed in service, the three mechanisms of iodine removal are degraded by the contaminants of the air at different rates; the adsorption process degrades faster than the other two. This behavior will be shown by comparisons of methyl iodide-127 and methyl iodide-131 penetration tests. It was found possible to regenerate the iodine isotope-exchange efficiency by reaction with airborne chemical reducing agents with little or no improvement in methyl iodine-127 retention. Examples will be given of the chemical regeneration of carbons after exhaustion with known contaminants as well as for many carbons removed from nuclear power operations. The depth profile of methyl iodide-131 penetration was determined in 2-inch deep layers before and after chemical treatments

  15. Mannosylated Chitosan Nanoparticles Based Macrophage-Targeting Gene Delivery System Enhanced Cellular Uptake and Improved Transfection Efficiency.

    Science.gov (United States)

    Peng, Yixing; Yao, Wenjun; Wang, Bo; Zong, Li

    2015-04-01

    Gene transfer mediated by mannosylated chitosan (MCS) is a safe and promising approach for gene and vaccine delivery. MCS nanoparticles based gene delivery system showed high in vivo delivery efficiency and elicited strong immune responses in mice. However, little knowledge about the cell binding, transfection efficiency and intracellular trafficking of MCS nanoparticles had been acquired. In this study, using gastrin-releasing peptide as a model plasmid (pGRP), the binding of MCS/pGRP nanoparticles to macrophages and the intracellular trafficking of MCS/pGRP nanoparticles in macrophages were investigated. MCS-mediated transfection efficiency in macrophages was also evaluated using pGL-3 as a reporter gene. The results showed that the binding and transfection efficiency of MCS nanoparticles in macrophages was higher than that of CS, which was attributed to the interaction between mannose ligands in MCS and mannose receptors on the surface of macrophages. Observation with a confocal laser scanning microscope indicated the cellular uptake of MCS/pGRP nanoparticles were more than that of CS/pGRP nanoparticles in macrophages. MCS/pGRP nanoparticles were taken up by macrophages and most of them were entrapped in endosomal/lysosomal compartments. After the nanoparticles escaping from endosomal/lysosomal compartments, naked pGRP entered the nucleus, and a few MCS might enter the nucleus in terms of nanoparticles. Overall, MCS has the potential to be an excellent macrophage-targeting gene delivery carrier.

  16. Analysis of the efficiency of some international projects in Ukraine for future of nuclear energy

    International Nuclear Information System (INIS)

    Radovskaya, O.; Pysmennaya, U.; Zenyuk, D.

    2001-01-01

    The report presents the review of the status and perspectives for nuclear energy development in Ukraine. The role of certain projects for young experts is evaluated - among these projects the basic one is the IAEA Technical Co-operation Project UKR/4/006 S trategy for Nuclear Energy Development , as well as projects of the Ukrainian Nuclear Society and the Institute of Nuclear and Energy Law. (authors)

  17. High-efficiency targets for high-gain inertial confinement fusion

    International Nuclear Information System (INIS)

    Gardner, J.H.; Bodner, S.E.

    1986-01-01

    Rocket efficiencies as high as 15% are possible using short wavelength lasers and moderately high aspect ratio pellet designs. These designs are made possible by two recent breakthroughs in physics constraints. First is the development of the induced spatial incoherence (ISI) technique, which allows uniform illumination of the pellet and relaxes the constraint of thermal smoothing, permitting the use of short wavelength laser light. Second is the discovery that the Rayleigh--Taylor growth rate is considerably reduced at short laser wavelengths. By taking advantage of the reduced constraints imposed by nonuniform laser illumination and Rayleigh--Taylor instability, pellets using (1)/(4) μm laser light and initial aspect ratios of about 10 (with in flight aspect ratios of about 150--200) may produce energy gains as high as 200--250

  18. Targeted NGS meets expert clinical characterization: Efficient diagnosis of spastic paraplegia type 11

    Directory of Open Access Journals (Sweden)

    Cristina Castro-Fernández

    2015-06-01

    Full Text Available Next generation sequencing (NGS is transforming the diagnostic approach for neurological disorders, since it allows simultaneous analysis of hundreds of genes, even based on just a broad, syndromic patient categorization. However, such an approach bears a high risk of incidental and uncertain genetic findings. We report a patient with spastic paraplegia whose comprehensive neurological and imaging examination raised a high clinical suspicion of SPG11. Thus, although our NGS pipeline for this group of disorders includes gene panel and exome sequencing, in this sample only the spatacsin gene region was captured and subsequently searched for mutations. Two probably pathogenic variants were quickly and clearly identified, confirming the diagnosis of SPG11. This case illustrates how combination of expert clinical characterization with highly oriented NGS protocols leads to a fast, cost-efficient diagnosis, minimizing the risk of findings with unclear significance.

  19. Enabling benchmarking and improving operational efficiency at nuclear power plants through adoption of a common process model: SNPM (standard nuclear performance model)

    International Nuclear Information System (INIS)

    Pete Karns

    2006-01-01

    others. The goal of the SNPM is to give the people maintaining and operating nuclear power stations a common model on which to base their business processes and measure/benchmark themselves against others. The importance of benchmarking and comparing 'apples to apples' has and will continue to safely drive improvement and efficiencies throughout the business. For example, in the mid 1990's it was quite difficult to compare work management statistics and programs between plants. The introduction of several INPO documents, which eventually became the SNPM work management process (AP 928) enabled plants to benchmark and compare information on many aspects of work management, in fact INPO began to evaluate the nuclear plants on their implementation and usage of AP 928. Also, the standardization enabled the identification and benchmarking of innovations in plant processes and performance, which in turn helped to facilitate those innovations being accepted in other plants-thus furthering the cycle of continuous improvement. Using a master plan, all communities of practice are able to identify specific improvement projects and coordinate the implementation of the processes to ensure smooth transitions between the various process interface or intersection points. In essence the nuclear energy industry in the United States is working as one company-driving efficiencies and operational improvements. Key enablers in adopting the best practices like the SNPM are work, asset and supply chain management solutions - both from a functional and a technological point of view. In addition to the importance of supporting industry best practices, there are two additional attributes a nuclear power operating company should evaluate regarding software solutions for work, asset, and supply chain management: breadth of assets managed, and the architecture of solution. (author)

  20. A lentivirus-free inducible CRISPR-Cas9 system for efficient targeting of human genes.

    Science.gov (United States)

    Bisht, Kamlesh; Grill, Sherilyn; Graniel, Jacqueline; Nandakumar, Jayakrishnan

    2017-08-01

    CRISPR-Cas9 is a cutting-edge tool for modifying genomes. The efficacy with which Cas9 recognizes its target has revolutionized the engineering of knockouts. However this efficacy complicates the knocking out of important genes in cultured cells. Unedited cells holding a survival advantage within an edited population can confound the knockout phenotype. Here we develop a HeLa-based system that overcomes this limitation, incorporating several attractive features. First, we use Flp-recombinase to generate clones stably integrated for Cas9 and guide RNAs, eliminating the possibility of unedited cells. Second, Cas9 can be induced uniformly in the clonal cultures using doxycycline to measure the knockout phenotype. Third, two genes can be simultaneously knocked out using this approach. Finally, by not involving lentiviruses, our method is appealing to a broad research audience. Using this methodology we generated an inducible AGO2-knockout cell line showing normal RNA interference in the absence of doxycycline. Upon induction of Cas9, the AGO2 locus was cleaved, the AGO2 protein was depleted, and RNA interference was compromised. In addition to generating inducible knockouts, our technology can be adapted to improve other applications of Cas9, including transcriptional/epigenetic modulation and visualization of cellular DNA loci. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  1. Mucoadhesive microemulsion of ibuprofen: design and evaluation for brain targeting efficiency through intranasal route

    Directory of Open Access Journals (Sweden)

    Surjyanarayan Mandal

    2015-09-01

    Full Text Available This study aimed at designing mucoadhesive microemulsion gel to enhance the brain uptake of Ibuprofen through intranasal route. Ibuprofen loaded mucoadhesive microemulsion (MMEI was developed by incorporating polycarbophil as mucoadhesive polymer into Capmul MCM based optimal microemulsion (MEI and was subjected to characterization, stability, mucoadhesion and naso-ciliotoxicity study. Brain uptake of ibuprofen via nasal route was studied by performing biodistribution study in Swiss albino rats. MEI was found to be transparent, stable and non ciliotoxic with 66.29 ± 4.15 nm, -20.9 ± 3.98 mV and 98.66 ± 1.01% as average globule size, zeta potential and drug content respectively. Transmission Electron Microscopy (TEM study revealed the narrow globule size distribution of MEI. Following single intranasal administration of MMEI and MEI at a dose of 2.86 mg/kg, uptake of ibuprofen in the olfactory bulb was around 3.0 and 1.7 folds compared with intravenous injection of ibuprofen solution (IDS. The ratios of AUC in brain tissues to that in plasma obtained after nasal administration of MMEI were significantly higher than those after intravenous administration of IDS. Findings of the present investigation revealed that the developed mucoadhesive microemulsion gel could be a promising approach for brain targeting of ibuprofen through intranasal route.

  2. Improved bioavailability of targeted Curcumin delivery efficiently regressed cardiac hypertrophy by modulating apoptotic load within cardiac microenvironment

    International Nuclear Information System (INIS)

    Ray, Aramita; Rana, Santanu; Banerjee, Durba; Mitra, Arkadeep; Datta, Ritwik; Naskar, Shaon; Sarkar, Sagartirtha

    2016-01-01

    Cardiomyocyte apoptosis acts as a prime modulator of cardiac hypertrophy leading to heart failure, a major cause of human mortality worldwide. Recent therapeutic interventions have focussed on translational applications of diverse pharmaceutical regimes among which, Curcumin (from Curcuma longa) is known to have an anti-hypertrophic potential but with limited pharmacological efficacies due to low aqueous solubility and poor bioavailability. In this study, Curcumin encapsulated by carboxymethyl chitosan (CMC) nanoparticle conjugated to a myocyte specific homing peptide was successfully delivered in bioactive form to pathological myocardium for effective regression of cardiac hypertrophy in a rat (Rattus norvegicus) model. Targeted nanotization showed higher cardiac bioavailability of Curcumin at a low dose of 5 mg/kg body weight compared to free Curcumin at 35 mg/kg body weight. Moreover, Curcumin/CMC-peptide treatment during hypertrophy significantly improved cardiac function by downregulating expression of hypertrophy marker genes (ANF, β-MHC), apoptotic mediators (Bax, Cytochrome-c) and activity of apoptotic markers (Caspase 3 and PARP); whereas free Curcumin in much higher dose showed minimal improvement during compromised cardiac function. Targeted Curcumin treatment significantly lowered p53 expression and activation in diseased myocardium via inhibited interaction of p53 with p300-HAT. Thus attenuated acetylation of p53 facilitated p53 ubiquitination and reduced the apoptotic load in hypertrophied cardiomyocytes; thereby limiting cardiomyocytes' need to enter the regeneration cycle during hypertrophy. This study elucidates for the first time an efficient targeted delivery regimen for Curcumin and also attributes towards probable mechanistic insight into its therapeutic potential as a cardio-protective agent for regression of cardiac hypertrophy. - Highlights: • Cardiomyocyte targeted Curcumin/CMC-peptide increases bioavailability of the drug.

  3. Reduced nighttime transpiration is a relevant breeding target for high water-use efficiency in grapevine.

    Science.gov (United States)

    Coupel-Ledru, Aude; Lebon, Eric; Christophe, Angélique; Gallo, Agustina; Gago, Pilar; Pantin, Florent; Doligez, Agnès; Simonneau, Thierry

    2016-08-09

    Increasing water scarcity challenges crop sustainability in many regions. As a consequence, the enhancement of transpiration efficiency (TE)-that is, the biomass produced per unit of water transpired-has become crucial in breeding programs. This could be achieved by reducing plant transpiration through a better closure of the stomatal pores at the leaf surface. However, this strategy generally also lowers growth, as stomatal opening is necessary for the capture of atmospheric CO2 that feeds daytime photosynthesis. Here, we considered the reduction in transpiration rate at night (En) as a possible strategy to limit water use without altering growth. For this purpose, we carried out a genetic analysis for En and TE in grapevine, a major crop in drought-prone areas. Using recently developed phenotyping facilities, potted plants of a cross between Syrah and Grenache cultivars were screened for 2 y under well-watered and moderate soil water deficit scenarios. High genetic variability was found for En under both scenarios and was primarily associated with residual diffusion through the stomata. Five quantitative trait loci (QTLs) were detected that underlay genetic variability in En Interestingly, four of them colocalized with QTLs for TE. Moreover, genotypes with favorable alleles on these common QTLs exhibited reduced En without altered growth. These results demonstrate the interest of breeding grapevine for lower water loss at night and pave the way to breeding other crops with this underexploited trait for higher TE.

  4. Challenges faced by engineering services group in meeting nuclear power project targets

    International Nuclear Information System (INIS)

    Phanse, N.R.

    2001-01-01

    Nuclear Power Corporation of India Ltd. (NPCIL) is an organisation building and operating nuclear power stations for which a number of different types of engineering services/activities are necessary. All these services are provided by Engineering Services Group of NPCIL. The activities and responsibilities of the group are discussed

  5. Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae

    Directory of Open Access Journals (Sweden)

    James A Nicholls

    2015-09-01

    Full Text Available Evolutionary radiations are prominent and pervasive across many plant lineages in diverse geographical and ecological settings; in neotropical rainforests there is growing evidence suggesting that a significant fraction of species richness is the result of recent radiations. Understanding the evolutionary trajectories and mechanisms underlying these radiations demands much greater phylogenetic resolution than is currently available for these groups. The neotropical tree genus Inga (Leguminosae is a good example, with ~300 extant species and a crown age of 2-10 MY, yet over 6kb of plastid and nuclear DNA sequence data gives only poor phylogenetic resolution among species. Here we explore the use of larger-scale nuclear gene data obtained though targeted enrichment to increase phylogenetic resolution within Inga. Transcriptome data from three Inga species were used to select 264 nuclear loci for targeted enrichment and sequencing. Following quality control to remove probable paralogs from these sequence data, the final dataset comprised 259,313 bases from 194 loci for 24 accessions representing 22 Inga species and an outgroup (Zygia. Bayesian phylogenies reconstructed using either all loci concatenated or a subset of 60 loci in a gene-tree/species-tree approach yielded highly resolved phylogenies. We used coalescent approaches to show that the same targeted enrichment data also have significant power to discriminate among alternative within-species population histories in the widespread species I. umbellifera. In either application, targeted enrichment simplifies the informatics challenge of identifying orthologous loci associated with de novo genome sequencing. We conclude that targeted enrichment provides the large volumes of phylogenetically-informative sequence data required to resolve relationships within recent plant species radiations, both at the species level and for within-species phylogeographic studies.

  6. LuIII parvovirus selectively and efficiently targets, replicates in, and kills human glioma cells.

    Science.gov (United States)

    Paglino, Justin C; Ozduman, Koray; van den Pol, Anthony N

    2012-07-01

    Because productive infection by parvoviruses requires cell division and is enhanced by oncogenic transformation, some parvoviruses may have potential utility in killing cancer cells. To identify the parvovirus(es) with the optimal oncolytic effect against human glioblastomas, we screened 12 parvoviruses at a high multiplicity of infection (MOI). MVMi, MVMc, MVM-G17, tumor virus X (TVX), canine parvovirus (CPV), porcine parvovirus (PPV), rat parvovirus 1A (RPV1A), and H-3 were relatively ineffective. The four viruses with the greatest oncolytic activity, LuIII, H-1, MVMp, and MVM-G52, were tested for the ability, at a low MOI, to progressively infect the culture over time, causing cell death at a rate higher than that of cell proliferation. LuIII alone was effective in all five human glioblastomas tested. H-1 progressively infected only two of five; MVMp and MVM-G52 were ineffective in all five. To investigate the underlying mechanism of LuIII's phenotype, we used recombinant parvoviruses with the LuIII capsid replacing the MVMp capsid or with molecular alteration of the P4 promoter. The LuIII capsid enhanced efficient replication and oncolysis in MO59J gliomas cells; other gliomas tested required the entire LuIII genome to exhibit enhanced infection. LuIII selectively infected glioma cells over normal glial cells in vitro. In mouse models, human glioblastoma xenografts were selectively infected by LuIII when administered intratumorally; LuIII reduced tumor growth by 75%. LuIII also had the capacity to selectively infect subcutaneous or intracranial gliomas after intravenous inoculation. Intravenous or intracranial LuIII caused no adverse effects. Intracranial LuIII caused no infection of mature mouse neurons or glia in vivo but showed a modest infection of developing neurons.

  7. Two-step membrane binding by the bacterial SRP receptor enable efficient and accurate Co-translational protein targeting.

    Science.gov (United States)

    Hwang Fu, Yu-Hsien; Huang, William Y C; Shen, Kuang; Groves, Jay T; Miller, Thomas; Shan, Shu-Ou

    2017-07-28

    The signal recognition particle (SRP) delivers ~30% of the proteome to the eukaryotic endoplasmic reticulum, or the bacterial plasma membrane. The precise mechanism by which the bacterial SRP receptor, FtsY, interacts with and is regulated at the target membrane remain unclear. Here, quantitative analysis of FtsY-lipid interactions at single-molecule resolution revealed a two-step mechanism in which FtsY initially contacts membrane via a Dynamic mode, followed by an SRP-induced conformational transition to a Stable mode that activates FtsY for downstream steps. Importantly, mutational analyses revealed extensive auto-inhibitory mechanisms that prevent free FtsY from engaging membrane in the Stable mode; an engineered FtsY pre-organized into the Stable mode led to indiscriminate targeting in vitro and disrupted FtsY function in vivo. Our results show that the two-step lipid-binding mechanism uncouples the membrane association of FtsY from its conformational activation, thus optimizing the balance between the efficiency and fidelity of co-translational protein targeting.

  8. Altered surfactant homeostasis and recurrent respiratory failure secondary to TTF-1 nuclear targeting defect.

    Science.gov (United States)

    Peca, Donatella; Petrini, Stefania; Tzialla, Chryssoula; Boldrini, Renata; Morini, Francesco; Stronati, Mauro; Carnielli, Virgilio P; Cogo, Paola E; Danhaive, Olivier

    2011-08-25

    Mutations of genes affecting surfactant homeostasis, such as SFTPB, SFTPC and ABCA3, lead to diffuse lung disease in neonates and children. Haploinsufficiency of NKX2.1, the gene encoding the thyroid transcription factor-1 (TTF-1)--critical for lung, thyroid and central nervous system morphogenesis and function--causes a rare form of progressive respiratory failure designated brain-lung-thyroid syndrome. Molecular mechanisms involved in this syndrome are heterogeneous and poorly explored. We report a novel TTF-1 molecular defect causing recurrent respiratory failure episodes in an infant. The subject was an infant with severe neonatal respiratory distress syndrome followed by recurrent respiratory failure episodes, hypopituitarism and neurological abnormalities. Lung histology and ultrastructure were assessed by surgical biopsy. Surfactant-related genes were studied by direct genomic DNA sequencing and array chromatine genomic hybridization (aCGH). Surfactant protein expression in lung tissue was analyzed by confocal immunofluorescence microscopy. For kinetics studies, surfactant protein B and disaturated phosphatidylcholine (DSPC) were isolated from serial tracheal aspirates after intravenous administration of stable isotope-labeled (2)H(2)O and (13)C-leucine; fractional synthetic rate was derived from gas chromatography/mass spectrometry (2)H and (13)C enrichment curves. Six intubated infants with no primary lung disease were used as controls. Lung biopsy showed desquamative interstitial pneumonitis and lamellar body abnormalities suggestive of genetic surfactant deficiency. Genetic studies identified a heterozygous ABCA3 mutation, L941P, previously unreported. No SFTPB, SFTPC or NKX2.1 mutations or deletions were found. However, immunofluorescence studies showed TTF-1 prevalently expressed in type II cell cytoplasm instead of nucleus, indicating defective nuclear targeting. This pattern has not been reported in human and was not found in two healthy controls and

  9. Altered surfactant homeostasis and recurrent respiratory failure secondary to TTF-1 nuclear targeting defect

    Directory of Open Access Journals (Sweden)

    Carnielli Virgilio P

    2011-08-01

    Full Text Available Abstract Background Mutations of genes affecting surfactant homeostasis, such as SFTPB, SFTPC and ABCA3, lead to diffuse lung disease in neonates and children. Haploinsufficiency of NKX2.1, the gene encoding the thyroid transcription factor-1 (TTF-1 - critical for lung, thyroid and central nervous system morphogenesis and function - causes a rare form of progressive respiratory failure designated brain-lung-thyroid syndrome. Molecular mechanisms involved in this syndrome are heterogeneous and poorly explored. We report a novel TTF-1 molecular defect causing recurrent respiratory failure episodes in an infant. Methods The subject was an infant with severe neonatal respiratory distress syndrome followed by recurrent respiratory failure episodes, hypopituitarism and neurological abnormalities. Lung histology and ultrastructure were assessed by surgical biopsy. Surfactant-related genes were studied by direct genomic DNA sequencing and array chromatine genomic hybridization (aCGH. Surfactant protein expression in lung tissue was analyzed by confocal immunofluorescence microscopy. For kinetics studies, surfactant protein B and disaturated phosphatidylcholine (DSPC were isolated from serial tracheal aspirates after intravenous administration of stable isotope-labeled 2H2O and 13C-leucine; fractional synthetic rate was derived from gas chromatography/mass spectrometry 2H and 13C enrichment curves. Six intubated infants with no primary lung disease were used as controls. Results Lung biopsy showed desquamative interstitial pneumonitis and lamellar body abnormalities suggestive of genetic surfactant deficiency. Genetic studies identified a heterozygous ABCA3 mutation, L941P, previously unreported. No SFTPB, SFTPC or NKX2.1 mutations or deletions were found. However, immunofluorescence studies showed TTF-1 prevalently expressed in type II cell cytoplasm instead of nucleus, indicating defective nuclear targeting. This pattern has not been reported in human

  10. A-type nuclear lamins act as transcriptional repressors when targeted to promoters

    International Nuclear Information System (INIS)

    Lee, Damian C.; Welton, K. Linnea; Smith, Erica D.; Kennedy, Brian K.

    2009-01-01

    Regions of heterochromatin are often found at the periphery of the mammalian nucleus, juxtaposed to the nuclear lamina. Genes in these regions are likely maintained in a transcriptionally silent state, although other locations at the nuclear periphery associated with nuclear pores are sites of active transcription. As primary components of the nuclear lamina, A- and B-type nuclear lamins are intermediate filament proteins that interact with DNA, histones and known transcriptional repressors, leading to speculation that they may promote establishment of repressive domains. However, no direct evidence of a role for nuclear lamins in transcriptional repression has been reported. Here we find that human lamin A, when expressed in yeast and cultured human cells as a fusion protein to the Gal4 DNA-binding domain (DBD), can mediate robust transcriptional repression of promoters with Gal4 binding sites. Full repression by lamin A requires both the coiled-coil rod domain and the C-terminal tail domain. In human cells, other intermediate filament proteins such as lamin B and vimentin are unable to confer robust repression as Gal4-DBD fusions, indicating that this property is specific to A-type nuclear lamins. These findings indicate that A-type lamins can promote transcriptional repression when in proximity of a promoter

  11. Human factor - an important reserve in increasing efficiency and safety of nuclear power plants

    International Nuclear Information System (INIS)

    Simunek, P.

    1982-01-01

    It is demonstrated that the relationship between man and technical equipment in a nuclear power plant should be studied using the systems analysis approach. The consistent use of ergonomic knowledge in nuclear power plants makes it possible with relatively small additional expenditure to achieve considerable economic effect. The establishment is therefore suggested of a workplace to coordinate the use of applied ergonomics in nuclear power plants. (Ha)

  12. Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding

    Energy Technology Data Exchange (ETDEWEB)

    Kitamura, Akira; Nakayama, Yusaku; Kinjo, Masataka, E-mail: kinjo@sci.hokudai.ac.jp

    2015-07-31

    Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells. However, the detailed mechanism of nuclear retention of GFP-NLS remains unclear. Here, we show that a candidate mechanism for the strong nuclear retention of GFP-NLS is via the RNA-binding ability of the NLS sequence. GFP tagged with a classical NLS derived from Simian virus 40 (GFP-NLS{sup SV40}) localized not only in the nucleoplasm, but also to the nucleolus, the nuclear subdomain in which ribosome biogenesis takes place. GFP-NLS{sup SV40} in the nucleolus was mobile, and intriguingly, the diffusion coefficient, which indicates the speed of diffusing molecules, was 1.5-fold slower than in the nucleoplasm. Fluorescence correlation spectroscopy (FCS) analysis showed that GFP-NLS{sup SV40} formed oligomers via RNA binding, the estimated molecular weight of which was larger than the limit for passive nuclear export into the cytoplasm. These findings suggest that the nuclear localization of GFP-NLS{sup SV40} likely results from oligomerization mediated via RNA binding. The analytical technique used here can be applied for elucidating the details of other nuclear localization mechanisms, including those of several types of nuclear proteins. In addition, GFP-NLS{sup SV40} can be used as an excellent marker for studying both the nucleoplasm and nucleolus in living cells. - Highlights: • Nuclear localization signal-tagged GFP (GFP-NLS) showed clear nuclear localization. • The GFP-NLS dynamically localized not only in the nucleoplasm, but also to the nucleolus. • The nuclear localization of GFP-NLS results from transient oligomerization mediated via RNA binding. • Our NLS-tagging procedure is ideal for use in artificial sequestration of proteins in the nucleus.

  13. Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding

    International Nuclear Information System (INIS)

    Kitamura, Akira; Nakayama, Yusaku; Kinjo, Masataka

    2015-01-01

    Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells. However, the detailed mechanism of nuclear retention of GFP-NLS remains unclear. Here, we show that a candidate mechanism for the strong nuclear retention of GFP-NLS is via the RNA-binding ability of the NLS sequence. GFP tagged with a classical NLS derived from Simian virus 40 (GFP-NLS SV40 ) localized not only in the nucleoplasm, but also to the nucleolus, the nuclear subdomain in which ribosome biogenesis takes place. GFP-NLS SV40 in the nucleolus was mobile, and intriguingly, the diffusion coefficient, which indicates the speed of diffusing molecules, was 1.5-fold slower than in the nucleoplasm. Fluorescence correlation spectroscopy (FCS) analysis showed that GFP-NLS SV40 formed oligomers via RNA binding, the estimated molecular weight of which was larger than the limit for passive nuclear export into the cytoplasm. These findings suggest that the nuclear localization of GFP-NLS SV40 likely results from oligomerization mediated via RNA binding. The analytical technique used here can be applied for elucidating the details of other nuclear localization mechanisms, including those of several types of nuclear proteins. In addition, GFP-NLS SV40 can be used as an excellent marker for studying both the nucleoplasm and nucleolus in living cells. - Highlights: • Nuclear localization signal-tagged GFP (GFP-NLS) showed clear nuclear localization. • The GFP-NLS dynamically localized not only in the nucleoplasm, but also to the nucleolus. • The nuclear localization of GFP-NLS results from transient oligomerization mediated via RNA binding. • Our NLS-tagging procedure is ideal for use in artificial sequestration of proteins in the nucleus

  14. Boron-Proton Nuclear-Fusion Enhancement Induced in Boron-Doped Silicon Targets by Low-Contrast Pulsed Laser

    Directory of Open Access Journals (Sweden)

    A. Picciotto

    2014-08-01

    Full Text Available We show that a spatially well-defined layer of boron dopants in a hydrogen-enriched silicon target allows the production of a high yield of alpha particles of around 10^{9} per steradian using a nanosecond, low-contrast laser pulse with a nominal intensity of approximately 3×10^{16}  W cm^{−2}. This result can be ascribed to the nature of the long laser-pulse interaction with the target and with the expanding plasma, as well as to the optimal target geometry and composition. The possibility of an impact on future applications such as nuclear fusion without production of neutron-induced radioactivity and compact ion accelerators is anticipated.

  15. Mitochondrially targeted vitamin E succinate efficiently kills breast tumour-initiating cells in a complex II-dependent manner

    International Nuclear Information System (INIS)

    Yan, Bing; Stantic, Marina; Zobalova, Renata; Bezawork-Geleta, Ayenachew; Stapelberg, Michael; Stursa, Jan; Prokopova, Katerina; Dong, Lanfeng; Neuzil, Jiri

    2015-01-01

    Accumulating evidence suggests that breast cancer involves tumour-initiating cells (TICs), which play a role in initiation, metastasis, therapeutic resistance and relapse of the disease. Emerging drugs that target TICs are becoming a focus of contemporary research. Mitocans, a group of compounds that induce apoptosis of cancer cells by destabilising their mitochondria, are showing their potential in killing TICs. In this project, we investigated mitochondrially targeted vitamin E succinate (MitoVES), a recently developed mitocan, for its in vitro and in vivo efficacy against TICs. The mammosphere model of breast TICs was established by culturing murine NeuTL and human MCF7 cells as spheres. This model was verified by stem cell marker expression, tumour initiation capacity and chemotherapeutic resistance. Cell susceptibility to MitoVES was assessed and the cell death pathway investigated. In vivo efficacy was studied by grafting NeuTL TICs to form syngeneic tumours. Mammospheres derived from NeuTL and MCF7 breast cancer cells were enriched in the level of stemness, and the sphere cells featured altered mitochondrial function. Sphere cultures were resistant to several established anti-cancer agents while they were susceptible to MitoVES. Killing of mammospheres was suppressed when the mitochondrial complex II, the molecular target of MitoVES, was knocked down. Importantly, MitoVES inhibited progression of syngeneic HER2 high tumours derived from breast TICs by inducing apoptosis in tumour cells. These results demonstrate that using mammospheres, a plausible model for studying TICs, drugs that target mitochondria efficiently kill breast tumour-initiating cells. The online version of this article (doi:10.1186/s12885-015-1394-7) contains supplementary material, which is available to authorized users

  16. A Study on intensifying efficiency for international collaborative development of Advanced Nuclear Energy Technology

    Energy Technology Data Exchange (ETDEWEB)

    Hahn, Dohee; Park, Seongwon; Chang, Moonhee

    2013-08-15

    All the countries of the world are promoting the use of atomic energy to provide against high oil prices, climatic changes, and energy security initiative. A domestic and foreign environment for nuclear energy is changing rapidly and 13 leading countries including Korea are trying to develop advanced technologies on Gen IV nuclear energy system through Gen IV International Forum (GIF). To enhance the effectiveness of the future nuclear energy system development plan, a strategic approach is necessary for GIF program and the connection process with the 4th Nuclear Energy Promotion Program and Nuclear Energy R and D Medium and Long Term 5 year Plan for 2012 ∼ 2016 needs to be prepared. This study was to analyze the global nuclear trends of 2012 and the status of GIF program which is international cooperation activities. Also we examined the domestic R and D status of future nuclear energy systems for developing core technology and commercialization of Gen-IV nuclear energy system. A successful performance of this project enables the effective national cooperation with GIF and promotes the public acceptance by suggesting the technical alternatives for the nuclear safety and the spent fuel management.

  17. Development of fission Mo-99 production technology - A nuclear feasibility study on UN target for Mo-99 production in HANARO

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Myung Hyun; Kim, Woo Sik [Kyunghee University, Seoul (Korea)

    2000-03-01

    Nuclear target design satisfying all the constraints for fission moly production in HANARO was proposed in this project. The 'MCNP-ORIGEN' code system which was previously proposed for a design tool, was evaluated by the comparison with through the 'MCNP-Analytic Eq.' system. A characteristics of each chemical processing step were analysed and material balance was set up to evaluate the overall yield ratio of Mo-99 recovery. A parametric study was done for the optimum HEU target design. Tested parameters were target thickness, recoil-loss rate to the fuel thickness, target radius, cladding materials, thickness of irradiation guide tube, and barrier materials. Optimized HEU target design was proposed which satisfying the constraints and having high production yield. For a LEU target design using 19.7 w/o UN powder fuel, a parametric study was also done for the optimization of fuel thickness, powder packing density, mixture material volume ratio. 24 refs., 35 figs., 57 tabs. (Author)

  18. Interactions of 10.6 GeV/n gold nuclei with light and heavy target nuclei in nuclear emulsion

    International Nuclear Information System (INIS)

    Cherry, M.L.; Denes-Jones, P.

    1994-03-01

    We have investigated the particle production and fragmentation of nuclei participating in the interactions of 10.6 GeV/n gold nuclei in nuclear emulsions. A new criteria has been developed to distinguish between the interactions of these gold nuclei with the light (H, C, N, O) and heavy (Ag, Br) target nuclei in the emulsion. This has allowed separate analyzes of the multiplicity and pseudo-rapidity distributions of the singly charged particles emitted in Au-(H, C, N, O) and Au-(Ag, Br) interactions, as well as of the models of breakup of the projectile and target nuclei. The pseudo-rapidity distributions show strong forward asymmetries, particularly for the interactions with the light nuclei. Heavy target nuclei produce a more severe breakup of the projectile gold nucleus than do the lighter targets. A negative correlation between the number of fragments emitted from the target nuclei and the degree of centrality of the collisions has been observed, which can be attributed to the total destruction of the relatively light target nuclei by these very heavy projectile nuclei. (author). 14 refs, 11 figs, 1 tab

  19. Deterring nuclear-armed Third World dictators: a targeting strategy for the emerging threat.

    OpenAIRE

    Gellene, David J.

    1992-01-01

    Approved for public release; distribution unlimited. The continuing efforts of several developing nations to acquire nuclear weapons indicates that the United States may be required to implement a deterrence policy aimed at authoritarian regimes in the Third World. Therefore. U. S. decision-makers must re-evaluate the conceptual foundations of American deterrence policy. This research suggests a solution to the problem of deterring nuclear-capable Third World nations from using...

  20. Evaluation of the effect of media velocity on filter efficiency and most penetrating particle size of nuclear grade high-efficiency particulate air filters.

    Science.gov (United States)

    Alderman, Steven L; Parsons, Michael S; Hogancamp, Kristina U; Waggoner, Charles A

    2008-11-01

    High-efficiency particulate air (HEPA) filters are widely used to control particulate matter emissions from processes that involve management or treatment of radioactive materials. Section FC of the American Society of Mechanical Engineers AG-1 Code on Nuclear Air and Gas Treatment currently restricts media velocity to a maximum of 2.5 cm/sec in any application where this standard is invoked. There is some desire to eliminate or increase this media velocity limit. A concern is that increasing media velocity will result in higher emissions of ultrafine particles; thus, it is unlikely that higher media velocities will be allowed without data to demonstrate the effect of media velocity on removal of ultrafine particles. In this study, the performance of nuclear grade HEPA filters, with respect to filter efficiency and most penetrating particle size, was evaluated as a function of media velocity. Deep-pleat nuclear grade HEPA filters (31 cm x 31 cm x 29 cm) were evaluated at media velocities ranging from 2.0 to 4.5 cm/sec using a potassium chloride aerosol challenge having a particle size distribution centered near the HEPA filter most penetrating particle size. Filters were challenged under two distinct mass loading rate regimes through the use of or exclusion of a 3 microm aerodynamic diameter cut point cyclone. Filter efficiency and most penetrating particle size measurements were made throughout the duration of filter testing. Filter efficiency measured at the onset of aerosol challenge was noted to decrease with increasing media velocity, with values ranging from 99.999 to 99.977%. The filter most penetrating particle size recorded at the onset of testing was noted to decrease slightly as media velocity was increased and was typically in the range of 110-130 nm. Although additional testing is needed, these findings indicate that filters operating at media velocities up to 4.5 cm/sec will meet or exceed current filter efficiency requirements. Additionally

  1. Nuclear Targets for a Precision Measurement of the Neutral Pion Radiative Width

    International Nuclear Information System (INIS)

    Martel, Philippe; Clinton, Eric; McWilliams, R.; Lawrence, Dave; Miskimen, Rory; Ahmidouch, Abdellah; Ambrozewicz, Pawel; Asaturyan, Arshak; Baker, O.; Benton, LaRay; Bernstein, Aron; Cole, Philip; Collins, Patrick; Dale, Daniel; Danagoulian, Samuel; Davidenko, G.; Demirchyan, Raphael; Deur, Alexandre; Dolgolenko, A.; Dzyubenko, Georgiy; Evdokimov, Anatoly; Feng, JIng; Gabrielyan, Marianna; Gan, Liping; Gasparian, Ashot; Glamazdin, Oleksandr; Goryachev, Vladimir; Gyurjyan, Vardan; Hardy, K.; Ito, Mark; Khandaker, Mahbubul; Kingsberry, Paul; Kolarkar, Ameya; Konchatnyi, Mykhailo; Korchin, O.; Korsch, Wolfgang; Kowalski, Stanley; Kubantsev, Mikhail; Kubarovsky, Valery; Larin, Ilya; Matveev, V.; McNulty, Dustin; Milbrath, Brian; Minehart, Ralph; Mochalov, Vasiliy; Mtingwa, Sekazi; Nakagawa, Itaru; Overby, Steven; Pasyuk, Evgueni; Payen, Marvin; Pedroni, Ronald; Prok, Yelena; Ritchie, Barry; Salgado, Carlos; Sitnikov, Anatoly; Sober, Daniel; Stephens, W.; Teymurazyan, Aram; Underwood, Jarreas; Vasiliev, A.; Verebryusov, V.; Vishnyakov, Vladimir; Wood, Michael

    2009-01-01

    A technique is presented for precision measurements of the area densities, density * T, of approximately 5% radiation length carbon and 208Pb targets used in an experiment at Jefferson Laboratory to measure the neutral pion radiative width. The precision obtained in the area density for the carbon target is +/- 0.050%, and that obtained for the lead target through an x-ray attenuation technique is +/- 0.43%.

  2. Nuclear Level Densities for Modeling Nuclear Reactions: An Efficient Approach Using Statistical Spectroscopy: Annual Scientific Report July 2004

    International Nuclear Information System (INIS)

    Calvin W. Johnson

    2004-01-01

    The general goal of the project is to develop and implement computer codes and input files to compute nuclear densities of state. Such densities are important input into calculations of statistical neutron capture, and are difficult to access experimentally. In particular, we will focus on calculating densities for nuclides in the mass range A ?????? 50 - 100. We use statistical spectroscopy, a moments method based upon a microscopic framework, the interacting shell model. In this report we present our progress for the past year

  3. Initial Screening of Thermochemical Water-Splitting Cycles for High Efficiency Generation of Hydrogen Fuels Using Nuclear Power

    International Nuclear Information System (INIS)

    Brown, L.C.; Funk, J.F.; Showalter, S.K.

    1999-01-01

    OAK B188 Initial Screening of Thermochemical Water-Splitting Cycles for High Efficiency Generation of Hydrogen Fuels Using Nuclear Power There is currently no large scale, cost-effective, environmentally attractive hydrogen production process, nor is such a process available for commercialization. Hydrogen is a promising energy carrier, which potentially could replace the fossil fuels used in the transportation sector of our economy. Fossil fuels are polluting and carbon dioxide emissions from their combustion are thought to be responsible for global warming. The purpose of this work is to determine the potential for efficient, cost-effective, large-scale production of hydrogen utilizing high temperature heat from an advanced nuclear power station. Almost 800 literature references were located which pertain to thermochemical production of hydrogen from water and over 100 thermochemical watersplitting cycles were examined. Using defined criteria and quantifiable metrics, 25 cycles have been selected for more detailed study

  4. Efficient prevention and compensation of catastrophic risks - the example of nuclear accidents

    International Nuclear Information System (INIS)

    Vanden Borre, T.

    2002-01-01

    The document is an abstract of a PhD thesis. The thesis critically reviews key elements of the current nuclear liability law, in particular the limitation of liability and discusses the benefits and disadvantages of nuclear insurance pools and collective systems of operators (captives)

  5. EDF decommissioning programme: A global commitment to safety, environment and cost efficiency of nuclear energy

    International Nuclear Information System (INIS)

    Grenouillet, J.-J.

    2002-01-01

    Nowadays, decommissioning of nuclear power plants has become a key issue for the nuclear industry in Europe. The phasing out of nuclear energy in Germany, Belgium and Sweden, as well as the early closure of nuclear units in applicant countries in the frame of EU enlargement, has largely contributed to consider decommissioning as the next challenge to face. The situation is slightly different in France: Nuclear energy is still considered as a safe, cost-effective and environment friendly energy source and EDF is still working on the development of a new generation of reactors to replace the existing ones. Nevertheless, to achieve this objective, it will be necessary to get the support of political decision-makers and the acceptance of public opinion. (author)

  6. The cell agglutination agent, phytohemagglutinin-L, improves the efficiency of somatic nuclear transfer cloning in cattle (Bos taurus).

    Science.gov (United States)

    Du, Fuliang; Shen, Perng-Chih; Xu, Jie; Sung, Li-Ying; Jeong, B-Seon; Lucky Nedambale, Tshimangadzo; Riesen, John; Cindy Tian, X; Cheng, Winston T K; Lee, Shan-Nan; Yang, Xiangzhong

    2006-02-01

    One of the several factors that contribute to the low efficiency of mammalian somatic cloning is poor fusion between the small somatic donor cell and the large recipient oocyte. This study was designed to test phytohemagglutinin (PHA) agglutination activity on fusion rate, and subsequent developmental potential of cloned bovine embryos. The toxicity of PHA was established by examining its effects on the development of parthenogenetic bovine oocytes treated with different doses (Experiment 1), and for different durations (Experiment 2). The effective dose and duration of PHA treatment (150 microg/mL, 20 min incubation) was selected and used to compare membrane fusion efficiency and embryo development following somatic cell nuclear transfer (Experiment 3). Cloning with somatic donor fibroblasts versus cumulus cells was also compared, both with and without PHA treatment (150 microg/mL, 20 min). Fusion rate of nuclear donor fibroblasts, after phytohemagglutinin treatment, was increased from 33 to 61% (P cell nuclear donors. The nuclear transfer (NT) efficiency per oocyte used was improved following PHA treatment, for both fibroblast (13% versus 22%) as well as cumulus cells (17% versus 34%; P cloned embryos, both with and without PHA treatment, were subjected to vitrification and embryo transfer testing, and resulted in similar survival (approximately 90% hatching) and pregnancy rates (17-25%). Three calves were born following vitrification and embryo transfer of these embryos; two from the PHA-treated group, and one from non-PHA control group. We concluded that PHA treatment significantly improved the fusion efficiency of somatic NT in cattle, and therefore, increased the development of cloned blastocysts. Furthermore, within a determined range of dose and duration, PHA had no detrimental effect on embryo survival post-vitrification, nor on pregnancy or calving rates following embryo transfer.

  7. Syringe shape and positioning relative to efficiency volume inside dose calibrators and its role in nuclear medicine quality assurance programs

    International Nuclear Information System (INIS)

    Santos, J.A.M.; Carrasco, M.F.; Lencart, J.; Bastos, A.L.

    2009-01-01

    A careful analysis of geometry and source positioning influence in the activity measurement outcome of a nuclear medicine dose calibrator is presented for 99m Tc. The implementation of a quasi-point source apparent activity curve measurement is proposed for an accurate correction of the activity inside several syringes, and compared with a theoretical geometric efficiency model. Additionally, new geometrical parameters are proposed to test and verify the correct positioning of the syringes as part of acceptance testing and quality control procedures.

  8. Japan's anti-nuclear weapons policy misses its target, even in the war on terrorism.

    Science.gov (United States)

    DiFilippo, Anthony

    2003-01-01

    While actively working to promote the abolition of all nuclear weapons from the world since the end of the cold war, Japan's disarmament policies are not without problems. Promoting the elimination of nuclear weapons as Japan remains under the US nuclear umbrella creates a major credibility problem for Tokyo, since this decision maintains a Japanese deterrence policy at the same time that officials push for disarmament. Tokyo also advocates a gradual approach to the abolition of nuclear weapons, a decision that has had no effect on those countries that have been conducting sub-critical nuclear testing, nor stopped India and Pakistan from carrying out nuclear tests. Consistent with Article 9 of the Constitution, the Japanese war-renouncing constitutional clause, Tokyo toughened Japan's sizeable Official Development Assistance (ODA) programme in the early 1990s. Because of the anti-military guidelines included in Japan's ODA programme, Tokyo stopped new grant and loan aid to India and Pakistan in 1998 after these countries conducted nuclear tests. However, because of the criticism Japan faced from its failure to participate in the 1991 Gulf War, Tokyo has been seeking a new Japanese role in international security during the post-cold war period. Deepening its commitment to the security alliance with the US, Tokyo has become increasingly influenced by Washington's global polices, including the American war on terrorism. After Washington decided that Pakistan would be a key player in the US war on terrorism, Tokyo restored grant and loan aid to both Islamabad and New Delhi, despite the unequivocal restrictions of Japan's ODA programme.

  9. Efficient extreme ultraviolet plasma source generated by a CO2 laser and a liquid xenon microjet target

    Science.gov (United States)

    Ueno, Yoshifumi; Ariga, Tatsuya; Soumagne, George; Higashiguchi, Takeshi; Kubodera, Shoichi; Pogorelsky, Igor; Pavlishin, Igor; Stolyarov, Daniil; Babzien, Marcus; Kusche, Karl; Yakimenko, Vitaly

    2007-05-01

    We demonstrated efficacy of a CO2-laser-produced xenon plasma in the extreme ultraviolet (EUV) spectral region at 13.5nm at variable laser pulse widths between 200ps and 25ns. The plasma target was a 30μm liquid xenon microjet. To ensure the optimum coupling of CO2 laser energy with the plasma, they applied a prepulse yttrium aluminum garnet laser. The authors measured the conversion efficiency (CE) of the 13.5nm EUV emission for different pulse widths of the CO2 laser. A maximum CE of 0.6% was obtained for a CO2 laser pulse width of 25ns at an intensity of 5×1010W/cm2.

  10. Efficient extreme ultraviolet plasma source generated by a CO2 laser and a liquid xenon microjet target

    International Nuclear Information System (INIS)

    Ueno, Yoshifumi; Ariga, Tatsuya; Soumagne, George; Higashiguchi, Takeshi; Kubodera, Shoichi; Pogorelsky, Igor; Pavlishin, Igor; Stolyarov, Daniil; Babzien, Marcus; Kusche, Karl; Yakimenko, Vitaly

    2007-01-01

    We demonstrated efficacy of a CO 2 -laser-produced xenon plasma in the extreme ultraviolet (EUV) spectral region at 13.5 nm at variable laser pulse widths between 200 ps and 25 ns. The plasma target was a 30 μm liquid xenon microjet. To ensure the optimum coupling of CO 2 laser energy with the plasma, they applied a prepulse yttrium aluminum garnet laser. The authors measured the conversion efficiency (CE) of the 13.5 nm EUV emission for different pulse widths of the CO 2 laser. A maximum CE of 0.6% was obtained for a CO 2 laser pulse width of 25 ns at an intensity of 5x10 10 W/cm 2

  11. First Nuclear Reaction Experiment with Stored Radioactive 56Ni Beam and Internal Hydrogen and Helium Targets

    NARCIS (Netherlands)

    Egelhof, P.; Bagchi, Soumya; Csatlós, M.; Dillmann, I.; Dimopoulou, C.; Furuno, T; Geissel, H.; Gernhauser, R.; Kalantar-Nayestanaki, Nasser; Kuilman, M.; Mahjour-Shafiei, M.; Najafi, M.A.; Rigollet, C.; Streicher, B.

    2014-01-01

    The investigation of light-ion induced direct reactions using stored and cooled radioactive beams, interacting with internal targets of storage rings, can lead to substantial advantages over external target experiments, in particular for direct reaction experiments in inverse kinematics at very low

  12. Efficient approach for determining four-dimensional computed tomography-based internal target volume in stereotactic radiotherapy of lung cancer

    International Nuclear Information System (INIS)

    Yeo, Seung Gu; Kim, Eun Seog

    2013-01-01

    This study aimed to investigate efficient approaches for determining internal target volume (ITV) from four-dimensional computed tomography (4D CT) images used in stereotactic body radiotherapy (SBRT) for patients with early-stage non-small cell lung cancer (NSCLC). 4D CT images were analyzed for 15 patients who received SBRT for stage I NSCLC. Three different ITVs were determined as follows: combining clinical target volume (CTV) from all 10 respiratory phases (ITV 10Phases ); combining CTV from four respiratory phases, including two extreme phases (0% and 50%) plus two intermediate phases (20% and 70%) (ITV 4Phases ); and combining CTV from two extreme phases (ITV 2Phases ). The matching index (MI) of ITV 4Phases and ITV 2Phases was defined as the ratio of ITV 4Phases and ITV 2Phases , respectively, to the ITV 10Phases . The tumor motion index (TMI) was defined as the ratio of ITV 10Phases to CTV mean , which was the mean of 10 CTVs delineated on 10 respiratory phases. The ITVs were significantly different in the order of ITV 10Phases , ITV 4Phases , and ITV 2Phases (all p 4Phases was significantly higher than that of ITV 2Phases (p 4Phases was inversely related to TMI (r = -0.569, p = 0.034). In a subgroup with low TMI (n = 7), ITV 4Phases was not statistically different from ITV 10Phases (p = 0.192) and its MI was significantly higher than that of ITV 2Phases (p = 0.016). The ITV 4Phases may be an efficient approach alternative to optimal ITV 10Phases in SBRT for early-stage NSCLC with less tumor motion.

  13. Low-debris, efficient laser-produced plasma extreme ultraviolet source by use of a regenerative liquid microjet target containing tin dioxide (SnO2) nanoparticles

    Science.gov (United States)

    Higashiguchi, Takeshi; Dojyo, Naoto; Hamada, Masaya; Sasaki, Wataru; Kubodera, Shoichi

    2006-05-01

    We demonstrated a low-debris, efficient laser-produced plasma extreme ultraviolet (EUV) source by use of a regenerative liquid microjet target containing tin-dioxide (SnO2) nanoparticles. By using a low SnO2 concentration (6%) solution and dual laser pulses for the plasma control, we observed the EUV conversion efficiency of 1.2% with undetectable debris.

  14. Low-debris, efficient laser-produced plasma extreme ultraviolet source by use of a regenerative liquid microjet target containing tin dioxide (SnO2) nanoparticles

    International Nuclear Information System (INIS)

    Higashiguchi, Takeshi; Dojyo, Naoto; Hamada, Masaya; Sasaki, Wataru; Kubodera, Shoichi

    2006-01-01

    We demonstrated a low-debris, efficient laser-produced plasma extreme ultraviolet (EUV) source by use of a regenerative liquid microjet target containing tin-dioxide (SnO 2 ) nanoparticles. By using a low SnO 2 concentration (6%) solution and dual laser pulses for the plasma control, we observed the EUV conversion efficiency of 1.2% with undetectable debris

  15. Study of the effect of irradiation of Mo targets at nuclear reactor

    International Nuclear Information System (INIS)

    Nieto, Renata C.; Lima, Ana Lucia V.P.; Silva, Nestor C. da; Osso Junior, Joao Alberto

    2000-01-01

    The most used radioisotope in nuclear medicine is 99m Tc, in the 99 Mo- 99m Tc generator form. 99 Mo can be produced by several nuclear reactions in reactors and cyclotrons. The cyclotron production is not technically and economically viable. The production in the reactor can be done in two different ways: by the fission of 235 U and by the 98 Mo(n,γ) 99 Mo reaction. A project for the production of 99 Mo by the activation of Mo and the preparation of gel type generators is under development at the 'Instituto de Pesquisas Energeticas e Nucleares'. In the present work, the radionuclidic impurities produced in the activation of MoO 3 , metallic Mo and Mo Zr gel were evaluated, as well as the radionuclidic purity of 99m Tc eluted from generators prepared. (author)

  16. Korea's emission trading scheme and policy design issues to achieve market-efficiency and abatement targets

    International Nuclear Information System (INIS)

    Park, Hojeong; Hong, Won Kyung

    2014-01-01

    In 2008, the government of Republic of Korea (Korea) announced the national abatement target aiming at 30% reductions from the Business-as-Usual projections by 2020. Accordingly, the Emission Trading Scheme (ETS) will be implemented from 2015 onwards. As ETS performance substantially depends on the structural design, it is critically important to examine the details of Korean ETS for the achievement of cost effectiveness and concurrent development of an active emission trading market. This paper addresses several policy design issues for this purpose. After providing an overview on the current framework of Korean ETS, we propose ways to achieve flexibility, consistency and market efficiency of the program in consideration of the preexisting policies. Issues in policy design are discussed by focusing on allowance allocation, market stabilization measures and price mechanism in the emission and energy markets in Korea. This paper will serve as a practical guideline for establishing sustainable and market-efficient Korean ETS that can be compatible with the international standards as in the EU ETS. - Highlights: • Emission Trading Scheme (ETS) will be implemented from 2015 in Korea to reduce CO 2 . • ETS performance substantially depends on structural design. • We provide policy overview on the current framework of Korean ETS. • Several policy design issues are discussed for developing policy consistency. • We focus on allowance allocation, allowance reserve and market stabilization measures

  17. Theranostic pH-sensitive nanoparticles for highly efficient targeted delivery of doxorubicin for breast tumor treatment.

    Science.gov (United States)

    Pan, Changqie; Liu, Yuqing; Zhou, Minyu; Wang, Wensheng; Shi, Min; Xing, Malcolm; Liao, Wangjun

    2018-01-01

    A multifunctional theranostic nanoplatform integrated with environmental responses has been developed rapidly over the past few years as a novel treatment strategy for several solid tumors. We synthesized pH-sensitive poly(β-thiopropionate) nanoparticles with a supermagnetic core and folic acid (FA) conjugation (FA-doxorubicin-iron oxide nanoparticles [FA-DOX@ IONPs]) to deliver an antineoplastic drug, DOX, for the treatment of folate receptor (FR)-overexpressed breast cancer. In addition to an imaging function, the nanoparticles can release their payloads in response to an environment of pH 5, such as the acidic environment found in tumors. After chemical ( 1 H nuclear magnetic resonance) and physical (morphology and super-magnetic) characterization, FA-DOX@IONPs were shown to demonstrate pH-dependent drug release profiles. Western blotting analysis revealed the expression of FRs in three breast cancer cell lines, MCF-7, BT549, and MD-MBA-231. The cell counting kit-8 assay and transmission electron microscopy showed that FA-DOX@IONPs had the strongest cytotoxicity against breast cancer cells, compared with free DOX and non-FR targeted nanoparticles (DOX@IONPs), and caused cellular apoptosis. The FA-DOX@IONP-mediated cellular uptake and intracellular internalization were clarified by fluorescence microscopy. FA-DOX@IONPs plus magnetic field treatment suppressed in vivo tumor growth in mice to a greater extent than either treatment alone; furthermore, the nanoparticles exerted no toxicity against healthy organs. Magnetic resonance imaging was successfully applied to monitor the nanoparticle accumulation. Our results suggest that theranostic pH-sensitive nanoparticles with dual targeting could enhance the available therapies for cancer.

  18. A study on the funding system for efficient implementation of nuclear R and D

    International Nuclear Information System (INIS)

    Chung, Whan Sam; Yang, M. H.; Song, K. D.; Kim, H. J.; Yun, S. W.

    2000-08-01

    This study is investigated and analysed as follows : first, investigation on the general account, special account, fund system as a financial source and deep analysis on charging fee of Nuclear R and D Fund; second, background of the establishment on Nuclear R and D Fund; third, investigation on the domestic and oversea's R and D Fund system; fourth, searching for the objective and basic guideline for the reshuffle of electric power industry and the issues on public charging fee; fifth, in depth analysis on the legal background and the main issues of Nuclear R and D Fund; and sixth, comprehensive arrangement on the main issues through the study

  19. Evaluation of an Efficient Approach for Target Tracking from Acoustic Imagery for the Perception System of an Autonomous Underwater Vehicle

    Directory of Open Access Journals (Sweden)

    Sebastián A. Villar

    2014-02-01

    Full Text Available This article describes the core algorithms of the perception system to be included within an autonomous underwater vehicle (AUV. This perception system is based on the acoustic data acquired from side scan sonar (SSS. These data should be processed in an efficient time, so that the perception system is able to detect and recognize a predefined target. This detection and recognition outcome is therefore an important piece of knowledge for the AUVs dynamic mission planner (DMP. Effectively, the DMP should propose different trajectories, navigation depths and other parameters that will change the robot's behaviour according to the perception system output. Hence, the time in which to make a decision is critical in order to assure safe robot operation and to acquire good quality data; consequently, the efficiency of the on-line image processing from acoustic data is a key issue. Current techniques for acoustic data processing are time and computationally intensive. Hence, it was decided to process data coming from a SSS using a technique that is used for radars, due to its efficiency and its amenability to on-line processing. The engineering problem to solve in this case was underwater pipeline tracking for routine inspections in the off-shore industry. Then, an automatic oil pipeline detection system was developed borrowing techniques from the processing of radar measurements. The radar technique is known as Cell Average – Constant False Alarm Rate (CA – CFAR. With a slight variation of the algorithms underlying this radar technique, which consisted of the previous accumulation of partial sums, a great improvement in computing time and effort was achieved. Finally, a comparison with previous approaches over images acquired with a SSS from a vessel in the Salvador de Bahia bay in Brazil showed the feasibility of using this on-board technique for AUV perception.

  20. Accumulation of the radionuclides in a target irradiated in the reactor of tajoura nuclear research center

    International Nuclear Information System (INIS)

    Abdunnobi, A.R.; Arebi, B.

    1998-01-01

    One of the main stages of radionuclides production in reactor is the distinguishing of a regime on target irradiation in order to acquire the sufficient activity and the purity of radioisotope required. The authors have derived formula for calculating radionuclidic accumulation on a target irradiated in the reactor operating 10 hours per day, 4 days a week during 4 weeks. The results of I-131 and other radionuclide accumulation are illustrated by a tellurium target irradiation in the reactor operating continuously or with interruptions

  1. Nuclear cereblon modulates transcriptional activity of Ikaros and regulates its downstream target, enkephalin, in human neuroblastoma cells

    International Nuclear Information System (INIS)

    Wada, Takeyoshi; Asahi, Toru; Sawamura, Naoya

    2016-01-01

    The gene coding cereblon (CRBN) was originally identified in genetic linkage analysis of mild autosomal recessive nonsyndromic intellectual disability. CRBN has broad localization in both the cytoplasm and nucleus. However, the significance of nuclear CRBN remains unknown. In the present study, we aimed to elucidate the role of CRBN in the nucleus. First, we generated a series of CRBN deletion mutants and determined the regions responsible for the nuclear localization. Only CRBN protein lacking the N-terminal region was localized outside of the nucleus, suggesting that the N-terminal region is important for its nuclear localization. CRBN was also identified as a thalidomide-binding protein and component of the cullin-4-containing E3 ubiquitin ligase complex. Thalidomide has been reported to be involved in the regulation of the transcription factor Ikaros by CRBN-mediated degradation. To investigate the nuclear functions of CRBN, we performed co-immunoprecipitation experiments and evaluated the binding of CRBN to Ikaros. As a result, we found that CRBN was associated with Ikaros protein, and the N-terminal region of CRBN was required for Ikaros binding. In luciferase reporter gene experiments, CRBN modulated transcriptional activity of Ikaros. Furthermore, we found that CRBN modulated Ikaros-mediated transcriptional repression of the proenkephalin gene by binding to its promoter region. These results suggest that CRBN binds to Ikaros via its N-terminal region and regulates transcriptional activities of Ikaros and its downstream target, enkephalin. - Highlights: • We found that CRBN is a nucleocytoplasmic shutting protein and identified the key domain for nucleocytoplasmic shuttling. • CRBN associates with the transcription factor Ikaros via the N-terminal domain. • CRBN modulates Ikaros-mediated transcriptional regulation and its downstream target, enkephalin.

  2. Nuclear cereblon modulates transcriptional activity of Ikaros and regulates its downstream target, enkephalin, in human neuroblastoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Wada, Takeyoshi [Faculty of Science and Engineering, Waseda University, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan); Asahi, Toru [Faculty of Science and Engineering, Waseda University, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan); Research Organization for Nano & Life Innovation, Waseda University #03C309, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan); Sawamura, Naoya, E-mail: naoya.sawamura@gmail.com [Faculty of Science and Engineering, Waseda University, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan); Research Organization for Nano & Life Innovation, Waseda University #03C309, TWIns, 2-2 Wakamatsu, Shinjuku, Tokyo, 162-8480 (Japan)

    2016-08-26

    The gene coding cereblon (CRBN) was originally identified in genetic linkage analysis of mild autosomal recessive nonsyndromic intellectual disability. CRBN has broad localization in both the cytoplasm and nucleus. However, the significance of nuclear CRBN remains unknown. In the present study, we aimed to elucidate the role of CRBN in the nucleus. First, we generated a series of CRBN deletion mutants and determined the regions responsible for the nuclear localization. Only CRBN protein lacking the N-terminal region was localized outside of the nucleus, suggesting that the N-terminal region is important for its nuclear localization. CRBN was also identified as a thalidomide-binding protein and component of the cullin-4-containing E3 ubiquitin ligase complex. Thalidomide has been reported to be involved in the regulation of the transcription factor Ikaros by CRBN-mediated degradation. To investigate the nuclear functions of CRBN, we performed co-immunoprecipitation experiments and evaluated the binding of CRBN to Ikaros. As a result, we found that CRBN was associated with Ikaros protein, and the N-terminal region of CRBN was required for Ikaros binding. In luciferase reporter gene experiments, CRBN modulated transcriptional activity of Ikaros. Furthermore, we found that CRBN modulated Ikaros-mediated transcriptional repression of the proenkephalin gene by binding to its promoter region. These results suggest that CRBN binds to Ikaros via its N-terminal region and regulates transcriptional activities of Ikaros and its downstream target, enkephalin. - Highlights: • We found that CRBN is a nucleocytoplasmic shutting protein and identified the key domain for nucleocytoplasmic shuttling. • CRBN associates with the transcription factor Ikaros via the N-terminal domain. • CRBN modulates Ikaros-mediated transcriptional regulation and its downstream target, enkephalin.

  3. Development of technologies on innovative-simplified nuclear power plant using high-efficiency steam injectors (2) analysis of heat balance of innovative-simplified nuclear power plant

    International Nuclear Information System (INIS)

    Goto, S.; Ohmori, S.; Mori, M.

    2005-01-01

    It is possible to establish simplified system with reduced space and total equipment weight using high-efficiency Steam Injector (SI) instead of low-pressure feedwater heaters in Nuclear Power Plant (NPP)(1)-(6). The SI works as a heat exchanger through direct contact between feedwater from the condensers and extracted steam from the turbines. It can get a higher pressure than supplied steam pressure, so it can reduce the feedwater pumps. The maintenance and reliability are still higher because SI has no movable parts. This paper describes the analysis of the heat balance and plant efficiency of this Innovative- Simplified NPP with high-efficiency SI. The plant efficiency is compared with the electric power of 1100MWe-class BWR system and the Innovative- Simplified BWR system with SI. The SI model is adapted into the heat balance simulator with a simplified model. The results show plant efficiencies of the Innovated-Simplified BWR system are almost equal to the original BWR one. The present research is one of the projects that are carried out by Tokyo Electric Power Company, Toshiba Corporation, and six Universities in Japan, funded from the Institute of Applied Energy (IAE) of Japan as the national public research-funded program. (authors)

  4. Development of technologies on innovative-simplified nuclear power plant using high-efficiency steam injectors. (2) Analysis of heat balance of innovative-simplified nuclear power plant

    International Nuclear Information System (INIS)

    Goto, Shoji; Ohmori, Shuichi; Mori, Mitchitsugu

    2004-01-01

    It is possible to established simplified systems and reduced space and equipments using high-efficiency Steam Injector (SI) instead of low-pressure feed water heaters in Nuclear Power Plant (NPP). The SI works as a heat exchanger through direct contact between feedwater from condenser and extracted steam from turbine. It can get a higher pressure than supplied steam pressure, so it can reduce the feedwater pumps. The maintenance and reliability are still higher because SI has no movable parts. This paper describes the analysis of the heat balance and plant efficiency of this Innovative-Simplified NPP with high-efficiency SI. The plant efficiency is compared with the electric power of 1100MWe class original BWR system and the Innovative-Simplified BWR system with SI. The SI model is adapted into the heat balance simulator with a simplified model. The results show plant efficiencies of the Innovated-Simplified BWR system are almost equal to the original BWR one. The present research is one of the projects that are carried out by Tokyo Electric Power Company, Toshiba Corporation, and six Universities in Japan, funded from the Institute of Applied Energy (IAE) of Japan as the national public research-funded program. (author)

  5. Abatement of CO2 emissions by way of enhancing the efficiency of nuclear power plants

    International Nuclear Information System (INIS)

    Kienle, F.

    1995-01-01

    Contributing about one third of the overall electricity supplied by the public utilities in 1994, nuclear power as in the previous years has been one of the major pillars of electricity supply in Germany. The approx. 150 billion kWh generated by the nuclear power plants represent reliable electricity supply around the clock, and free of CO 2 emissions, or SO 2 emissions, or NO x . Comparing nuclear generation with the electricity output contributed by conventional power plants in Germany, nuclear generation can also be expressed in terms of emissions avoided, which in 1994 meant: almost 150 million tons of CO 2 , equivalent to about 16 % of the aggregate annual CO 2 emissions; 110.000 tons of SO 2 , equivalent to about 11 % of aggregate annual SO 2 emissions; 125.000 tons of NO x , equivalent to 5 % of aggregate, annual NO x emissions. (orig.) [de

  6. Efficient power generation from large 7500C heat sources. Application to coal-fired and nuclear power station

    International Nuclear Information System (INIS)

    Tilliette, Z.P.; Pierre, B.

    1980-03-01

    Considering the future concern about a more efficient, rational use of heat sources, and also about a greater location flexibility of power plants owing to dry cooling possibility, closed gas cycles can offer new solutions for fossil or nuclear energy. An efficient heat conversion into power is obtained by the combination of a main non-intercooled helium cycle with a flexible, superheated, low pressure bottoming steam cycle. Emphasis is placed on the matching of the two cycle; for that, a recuperator by-pass arrangement is used. The operation of the main gas turbocompressor does not depend upon the operation of the small steam cycle. Results are given for a conservative turbine inlet temperature of 750 0 C. Applications are made to a coal-fired power plant and to a gas turbine, gas-cooled nuclear reactor. Overall net plant efficiencies of 39 per cent and 46 per cent respectively are reached. For a cycle top temperature equal to 850 0 C, corresponding net efficiencies would be 42 and 49 per cent

  7. Nuclear Imaging of Prostate Cancer with Gastrin-Releasing-Peptide-Receptor Targeted Radiopharmaceuticals

    NARCIS (Netherlands)

    Ananias, H. J. K.; de Jong, I. J.; Dierckx, R. A.; van de Wiele, C.; Helfrich, W.; Elsinga, P. H.

    2008-01-01

    Prostate cancer is one of the most common causes of cancer in men. Evaluating the different stages of prostate cancer with conventional imaging techniques still proves difficult. Nuclear imaging might provide a technique that is able to evaluate prostate cancer, but clinical application has been

  8. Improved calculations of the electronic and nuclear energy losses for light ions penetrating H and He targets at intermediate velocities

    Energy Technology Data Exchange (ETDEWEB)

    Grande, P.L. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Inst. de Fisica; Schiwietz, G. [Hahn-Meitner-Institut Berlin GmbH (Germany). Dept. FD

    1994-06-01

    A review is given on the use of the coupled-channel method to calculate the electronic and nuclear energy loss of ions penetrating the matter. This first principle calculation based on an expansion of the time dependent electronic wavefunction in terms of atomic orbitals has been applied to evaluate the impact parameter dependence of the electronic energy loss, the stopping cross-section and the fluctuation is energy loss of ions colliding with H and He atoms at energies of 10 keV/amu to 500 keV/amu. The results have been compared to experimental data as well as to others existing models, local density approximation in an electron gas target, harmonic oscillator target treatment and first order plane-wave-Born approximation. (author). 63 refs, 11 figs.

  9. Mathematics model of filtration efficiency of moisture separator for nuclear reactors

    International Nuclear Information System (INIS)

    Zhang Zhenzhong; Jiang Feng; Huang Yunfeng

    2010-01-01

    In order to study the filtration mechanism of the moisture separator for water droplet of 5∼10μm, this paper set up a physical model. For the mixed meshes, they can be classified into three types: standard meshes, bur meshes and middle meshes. For all fibers of the wire meshes and vertical fibers of standard mixed meshes, a Kuwabara flow field is used to track the particle to get the inertial impaction efficiency and then calculate the total filtration efficiency of the meshes. For other fibers, besides the Kuwabara flow field, an around-flat flow field is added to calculate the efficiency. Lastly, the total efficiency of the moisture separator according to the equation of the filtration efficiency for the filters in series is compared with the experimental data. The result shows that, under the standard condition,the calculation value is consistent with the experimental efficiency data. (authors)

  10. Ancient Origin of the U2 Small Nuclear RNA Gene-Targeting Non-LTR Retrotransposons Utopia.

    Science.gov (United States)

    Kojima, Kenji K; Jurka, Jerzy

    2015-01-01

    Most non-long terminal repeat (non-LTR) retrotransposons encoding a restriction-like endonuclease show target-specific integration into repetitive sequences such as ribosomal RNA genes and microsatellites. However, only a few target-specific lineages of non-LTR retrotransposons are distributed widely and no lineage is found across the eukaryotic kingdoms. Here we report the most widely distributed lineage of target sequence-specific non-LTR retrotransposons, designated Utopia. Utopia is found in three supergroups of eukaryotes: Amoebozoa, SAR, and Opisthokonta. Utopia is inserted into a specific site of U2 small nuclear RNA genes with different strength of specificity for each family. Utopia families from oomycetes and wasps show strong target specificity while only a small number of Utopia copies from reptiles are flanked with U2 snRNA genes. Oomycete Utopia families contain an "archaeal" RNase H domain upstream of reverse transcriptase (RT), which likely originated from a plant RNase H gene. Analysis of Utopia from oomycetes indicates that multiple lineages of Utopia have been maintained inside of U2 genes with few copy numbers. Phylogenetic analysis of RT suggests the monophyly of Utopia, and it likely dates back to the early evolution of eukaryotes.

  11. Radioactive Barium Ion Trap Based on Metal-Organic Framework for Efficient and Irreversible Removal of Barium from Nuclear Wastewater.

    Science.gov (United States)

    Peng, Yaguang; Huang, Hongliang; Liu, Dahuan; Zhong, Chongli

    2016-04-06

    Highly efficient and irreversible capture of radioactive barium from aqueous media remains a serious task for nuclear waste disposal and environmental protection. To address this task, here we propose a concept of barium ion trap based on metal-organic framework (MOF) with a strong barium-chelating group (sulfate and sulfonic acid group) in the pore structures of MOFs. The functionalized MOF-based ion traps can remove >90% of the barium within the first 5 min, and the removal efficiency reaches 99% after equilibrium. Remarkably, the sulfate-group-functionalized ion trap demonstrates a high barium uptake capacity of 131.1 mg g(-1), which surpasses most of the reported sorbents and can selectively capture barium from nuclear wastewater, whereas the sulfonic-acid-group-functionalized ion trap exhibits ultrafast kinetics with a kinetic rate constant k2 of 27.77 g mg(-1) min(-1), which is 1-3 orders of magnitude higher than existing sorbents. Both of the two MOF-based ion traps can capture barium irreversibly. Our work proposes a new strategy to design barium adsorbent materials and provides a new perspective for removing radioactive barium and other radionuclides from nuclear wastewater for environment remediation. Besides, the concrete mechanisms of barium-sorbent interactions are also demonstrated in this contribution.

  12. Conceptual design of a hydrogen production system by DME steam reforming and high-efficiency nuclear reactor technology

    International Nuclear Information System (INIS)

    Fukushima, Kimichika; Ogawa, Takashi

    2003-01-01

    Hydrogen is a potential alternative energy source and produced commercially by methane (natural gas) or LPG steam reforming, a process that requires high temperatures, which are produced by burning fossil fuels. However, since this process emits large amounts of CO 2 , replacement of the combustion heat source with a nuclear heat source for 773-1173 K processes has been proposed in order to eliminate these CO 2 emissions. This paper proposes a novel method of low-temperature nuclear hydrogen production by reforming dimethyl ether (DME) with steam produced by a low-temperature nuclear reactor at about 573 K. The authors identified conditions that provide high hydrogen production fraction at low pressure and temperatures of about 523-573 K. By setting this low-temperature hydrogen production process at about 573K upstream from a turbine, it was found theoretically that the total energy utilization efficiency is about 50% and very high. By setting a turbine upstream of the hydrogen production plant, an overall efficiency of is 75% for an FBR and 76% for a supercritical-water cooled power reactor (SCPR). (author)

  13. Strategies for safe and cost-efficient management of nuclear wastes in Finland

    International Nuclear Information System (INIS)

    Ryhanen, V.

    2002-01-01

    Full text: In 2001, the Finnish Parliament ratified the Decision-in-Principle on a final disposal facility of spent fuel to be constructed at the Olkiluoto site in the Western Finland. Since the early 1980s, this decision was grounded up by R and D and siting activities as well as by communications aiming at confidence building. Before the recent important licensing and siting step in the national waste management programme, interim storage facilities for spent fuel have been constructed at both nuclear power plant sites. Two shallow underground repositories were commissioned for low- and medium-level operating waste in the 1990s. Long-term programme and stepwise advancement have facilitated keeping the annual costs of nuclear waste management moderate. All major objectives were decided by the Government already in an early phase several decades ago, and these objectives have been met without significant delays. Nuclear power plant areas have been found out to be suitable even for location of waste storage and disposal facilities, which means benefits concerning infrastructure. Funds for R and D and implementation of waste management have been collected in the price of nuclear electricity since the start of nuclear power generation. (author)

  14. A Study on intensifying efficiency for international collaborative development of Advanced Nuclear Energy Technology

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Moon Hee; Kim, H. R.; Kim, H. J.; Chang, J. H.; Hahn, D. H.; Bae, Y. Y.; Kim, W. W.; Jeong, I.; Lee, D. S.; Lee, J. H. [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2008-06-15

    Generation IV International Forum(GIF), where 13 countries including Korea collaborate to develop future nuclear energy systems, put into force 'Generation IV International Forum Project Arrangement' in 2007 for the international research and development of Gen IV Systems, following the entry into force of Framework Agreement in 2005. The International Nuclear Research Initiative(I-NERI) between Korea and United States and the International Project on Innovative Nuclear Energy Systems and Fuel Cycles(INPRO) of IAEA are continued in this year, produced lots of visible outcomes. These international activities have a common goal of the collaborative development of advanced nuclear system technologies but differ in the main focusing areas and aspects, so Korea needs to establish the integrated strategy based on the distinguished and complementary approach for the participation of each international programs, as examples the GIF for the advanced system technology development, INPRO for the set-up of institution and infra-structure, and I-NERI for the access of the core technologies and acquisition of the transparency of nuclear R and D.

  15. A Study on intensifying efficiency for international collaborative development of Advanced Nuclear Energy Technology

    International Nuclear Information System (INIS)

    Chang, Moon Hee; Kim, H. R.; Kim, H. J.; Chang, J. H.; Hahn, D. H.; Bae, Y. Y.; Kim, W. W.; Jeong, I.; Lee, D. S.; Lee, J. H.

    2008-06-01

    Generation IV International Forum(GIF), where 13 countries including Korea collaborate to develop future nuclear energy systems, put into force 'Generation IV International Forum Project Arrangement' in 2007 for the international research and development of Gen IV Systems, following the entry into force of Framework Agreement in 2005. The International Nuclear Research Initiative(I-NERI) between Korea and United States and the International Project on Innovative Nuclear Energy Systems and Fuel Cycles(INPRO) of IAEA are continued in this year, produced lots of visible outcomes. These international activities have a common goal of the collaborative development of advanced nuclear system technologies but differ in the main focusing areas and aspects, so Korea needs to establish the integrated strategy based on the distinguished and complementary approach for the participation of each international programs, as examples the GIF for the advanced system technology development, INPRO for the set-up of institution and infra-structure, and I-NERI for the access of the core technologies and acquisition of the transparency of nuclear R and D

  16. Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts

    Directory of Open Access Journals (Sweden)

    Toshie Sakuma

    2014-03-01

    Full Text Available Previously we reported that nuclear export of both unspliced and spliced murine leukemia virus (MLV transcripts depends on the nuclear export factor (NXF1 pathway. Although the mRNA export complex TREX, which contains Aly/REF, UAP56, and the THO complex, is involved in the NXF1-mediated nuclear export of cellular mRNAs, its contribution to the export of MLV mRNA transcripts remains poorly understood. Here, we studied the involvement of TREX components in the export of MLV transcripts. Depletion of UAP56, but not Aly/REF, reduced the level of both unspliced and spliced viral transcripts in the cytoplasm. Interestingly, depletion of THO components, including THOC5 and THOC7, affected only unspliced viral transcripts in the cytoplasm. Moreover, the RNA immunoprecipitation assay showed that only the unspliced viral transcript interacted with THOC5. These results imply that MLV requires UAP56, THOC5 and THOC7, in addition to NXF1, for nuclear export of viral transcripts. Given that naturally intronless mRNAs, but not bulk mRNAs, require THOC5 for nuclear export, it is plausible that THOC5 plays a key role in the export of unspliced MLV transcripts.

  17. Abundant off-target edits from site-directed RNA editing can be reduced by nuclear localization of the editing enzyme.

    Science.gov (United States)

    Vallecillo-Viejo, Isabel C; Liscovitch-Brauer, Noa; Montiel-Gonzalez, Maria Fernanda; Eisenberg, Eli; Rosenthal, Joshua J C

    2018-01-02

    Site-directed RNA editing (SDRE) is a general strategy for making targeted base changes in RNA molecules. Although the approach is relatively new, several groups, including our own, have been working on its development. The basic strategy has been to couple the catalytic domain of an adenosine (A) to inosine (I) RNA editing enzyme to a guide RNA that is used for targeting. Although highly efficient on-target editing has been reported, off-target events have not been rigorously quantified. In this report we target premature termination codons (PTCs) in messages encoding both a fluorescent reporter protein and the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein transiently transfected into human epithelial cells. We demonstrate that while on-target editing is efficient, off-target editing is extensive, both within the targeted message and across the entire transcriptome of the transfected cells. By redirecting the editing enzymes from the cytoplasm to the nucleus, off-target editing is reduced without compromising the on-target editing efficiency. The addition of the E488Q mutation to the editing enzymes, a common strategy for increasing on-target editing efficiency, causes a tremendous increase in off-target editing. These results underscore the need to reduce promiscuity in current approaches to SDRE.

  18. Simple and effective method for nuclear tellurium isomers separation from antimony cyclotron targets

    International Nuclear Information System (INIS)

    Bondarevskij, S.I.; Eremin, V.V.

    1999-01-01

    Simple and effective method of generation of tellurium nuclear isomers from irradiated on cyclotron metallic antimony is suggested. Basically this method consists in consideration of the big difference in volatilities of metallic forms of antimony, tin and tellurium. Heating of the tin-antimony alloy at 1200 K permits to separate about 90 % of produced quantity of 121m Te and 123m Te (in this case impurity of antimony radionuclides is not more than 1 % on activity) [ru

  19. ATTILA (All Type Target Independent Lund Algorithm) for nuclear collisions at ≅ 200 AGeV

    International Nuclear Information System (INIS)

    Gyulassy, M.; Lawrence Berkeley Lab., CA

    1987-08-01

    The Lund string model, Fritiof, is applied in the form of a Monte-Carlo event generator, ATTILA, to nuclear collisions in the 200 AGeV range. We study how uncertainties at the pp level amplify at the AB level and compare preliminary CERN data on multiplicity, transverse energy, and veto energy distributions to this linear extrapolation scheme from pp to AB dynamics. (orig.)

  20. Target study of heat supply from Northern Moravia nuclear power plant

    International Nuclear Information System (INIS)

    Pospisil, V.

    The construction is envisaged in Northern Moravia of a nuclear power plant near Blahutovice in the Novy Jicin district. Heat produced by the nuclear power plant will only be used for district heating; process heat will be supplied from local steam sources. An example is discussed of the Prerov locality which currently is supplied from the Prerov heating and power plant (230 MW), a heating plant (36 MW) and from local sources (15 NW). The study estimates that a thermal power of 430 MW will be required at a time of the start of heat supplies from the nuclear power plant. All heat supply pipelines will be designed as a two-tube system divided into sections with section pipe fittings. The number and location of pipe fittings will be selected depending on the terrain configuration. Water of the maximum outlet temperature of 150 degC will be used as a coolant. The control of the system for Northern Moravia is briefly described. (J.P.)

  1. Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus.

    Science.gov (United States)

    Yang, Ying-Jie; Wang, Ye; Li, Zhi-Feng; Gong, Ya; Zhang, Peng; Hu, Wen-Chao; Sheng, Duo-Hong; Li, Yue-Zhong

    2017-08-16

    The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potential off-target DNA cleavage from incorrect guide by the 20 nt spacer. In this study, after resolving some critical limits, we have established an efficient CRISPR/Cas9 system for the deletion of large genome fragments related to the biosynthesis of secondary metabolites in Myxococcus xanthus cells. We revealed that the high expression of a codon-optimized cas9 gene in M. xanthus was cytotoxic, and developed a temporally high expression strategy to reduce the cell damage from high expressions of Cas9. We optimized the deletion protocol by using the tRNA-sgRNA-tRNA chimeric structure to ensure correct sgRNA sequence. We found that, in addition to the position-dependent nucleotide preference, the free energy of a 20 nt spacer was a key factor for the deletion efficiency. By using the developed protocol, we achieved the CRISPR/Cas9-induced deletion of large biosynthetic gene clusters for secondary metabolites in M. xanthus DK1622 and its epothilone-producing mutant. The findings and the proposals described in this paper were suggested to be workable in other organisms, for example, other Gram negative bacteria with high GC content.

  2. Significant improvement of pig cloning efficiency by treatment with LBH589 after somatic cell nuclear transfer.

    Science.gov (United States)

    Jin, Jun-Xue; Li, Suo; Gao, Qing-Shan; Hong, Yu; Jin, Long; Zhu, Hai-Ying; Yan, Chang-Guo; Kang, Jin-Dan; Yin, Xi-Jun

    2013-10-01

    The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) associates with epigenetic aberrancy, including the abnormal acetylation of histones. Altering the epigenetic status by histone deacetylase inhibitors (HDACi) enhances the developmental potential of SCNT embryos. In the current study, we examined the effects of LBH589 (panobinostat), a novel broad-spectrum HDACi, on the nuclear reprogramming and development of pig SCNT embryos in vitro. In experiment 1, we compared the in vitro developmental competence of nuclear transfer embryos treated with different concentrations of LBH589. Embryos treated with 50 nM LBH589 for 24 hours showed a significant increase in the rate of blastocyst formation compared with the control or embryos treated with 5 or 500 nM LBH589 (32.4% vs. 11.8%, 12.1%, and 10.0%, respectively, P < 0.05). In experiment 2, we examined the in vitro developmental competence of nuclear transfer embryos treated with 50 nM LBH589 for various intervals after activation and 6-dimethylaminopurine. Embryos treated for 24 hours had higher rates of blastocyst formation than the other groups. In experiment 3, when the acetylation of H4K12 was examined in SCNT embryos treated for 6 hours with 50 nM LBH589 by immunohistochemistry, the staining intensities of these proteins in LBH589-treated SCNT embryos were significantly higher than in the control. In experiment 4, LBH589-treated nuclear transfer and control embryos were transferred into surrogate mothers, resulting in three (100%) and two (66.7%) pregnancies, respectively. In conclusion, LBH589 enhances the nuclear reprogramming and developmental potential of SCNT embryos by altering the epigenetic status and expression, and increasing blastocyst quality. Crown Copyright © 2013. Published by Elsevier Inc. All rights reserved.

  3. NELCAS, an efficient and relevant tool to assess key environmental indicators for nuclear energy - 5334

    International Nuclear Information System (INIS)

    Bourg, S.; Poinssot, C.; Ouvrier, N.; Serp, J.

    2015-01-01

    Full text of publication follows. Nuclear energy is anticipated to be one of the possible energy sources which can allow the production of energy at high load with a high level of reliability without significant impact on the environment, specifically in terms of green-house gases. It could therefore promote the mitigation of the anticipated global climate change. However, nuclear energy is regularly questioned about its overall environmental impact and footprint, in particular after the Fukushima accident. However, very little information is available on the actual footprint of current and future nuclear systems. In order to bring insights on this issue, we developed a Life Cycle Assessment (LCA) simulation tool NELCAS based on the French nuclear system which allows the calculation of representative key environmental indicators and potential impact indicators for the whole nuclear systems. Indicators were chosen based on their occurrence in literature. Calculations were performed while considering the whole fuel cycle from ore-mining to geological repository and for each of the plant, the contribution from their construction, their operation and their future cleaning and dismantling. Transports were also considered all along the fuel cycle. All the matters and energy fluxes were considered and normalized versus the electric production. Whenever it is possible, actual and recent data were collected. In particular we widely used the Transparency and Safety Annual reports issued for each fuel cycle plant in France. Finally, statistical influence of the severe accidents was also considered based on the recent Fukushima accident. This presentation will detail the methodology and the overall results obtained for the different indicators. A specific emphasis will be given on the relative benefice of the nuclear energy by comparison to other energy sources. (authors)

  4. Intranuclear biophotonics by smart design of nuclear-targeting photo-/radio-sensitizers co-loaded upconversion nanoparticles.

    Science.gov (United States)

    Fan, Wenpei; Shen, Bo; Bu, Wenbo; Zheng, Xiangpeng; He, Qianjun; Cui, Zhaowen; Ni, Dalong; Zhao, Kuaile; Zhang, Shengjian; Shi, Jianlin

    2015-11-01

    Biophotonic technology that uses light and ionizing radiation for positioned cancer therapy is a holy grail in the field of biomedicine because it can overcome the systemic toxicity and adverse side effects of conventional chemotherapy. However, the existing biophotonic techniques fail to achieve the satisfactory treatment efficacy, which remains a big challenge for clinical implementation. Herein, we develop a novel theranostic technique of "intranuclear biophotonics" by the smart design of a nuclear-targeting biophotonic system based on photo-/radio-sensitizers covalently co-loaded upconversion nanoparticles. These nuclear-targeting biophotonic agents can not only generate a great deal of multiple cytotoxic reactive oxygen species in the nucleus by making full use of NIR/X-ray irradiation, but also produce greatly enhanced intranuclear synergetic radio-/photodynamic therapeutic effects under the magnetic/luminescent bimodal imaging guidance, which may achieve the optimal efficacy in treating radio-resistant tumors. We anticipate that the highly effective intranuclear biophotonics will contribute significantly to the development of biophotonic techniques and open new perspectives for a variety of cancer theranostic applications. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Nuclear plant analyzer: An efficient tool for training and operational analyses

    International Nuclear Information System (INIS)

    Bartsoen, L.; Mandy, C.; Stubbe, E.

    1999-01-01

    The advanced computer technology available now at low cost, combined with the maturity of the best-estimate engineering codes are the fundamentals of the Nuclear Plant Analyzer (NPA). At Tractebel Energy Engineering (TEE), the RELAP5 advanced thermal-hydraulics code is used as basis for the NPA that is mainly used for the training of simulator instructors and plant personnel. Using the special graphical features of the NPA, a set of six course modules has been prepared to provide an in-depth physical understanding of the main thermal-hydraulic phenomena that dominate nuclear power plant behavior in normal and accidental plant conditions. (author)

  6. More efficient integrated safeguards by applying a reasonable detection probability for maintaining low presence probability of undetected nuclear proliferating activities

    International Nuclear Information System (INIS)

    Otsuka, Naoto

    2013-01-01

    Highlights: • A theoretical foundation is presented for more efficient Integrated Safeguards (IS). • Probability of undetected nuclear proliferation activities should be maintained low. • For nations under IS, the probability to start proliferation activities is very low. • The fact can decrease the detection probability of IS by dozens of percentage points. • The cost of IS per nation can be cut down by reducing inspection frequencies etc. - Abstract: A theoretical foundation is presented for implementing more efficiently the present International Atomic Energy Agency (IAEA) integrated safeguards (ISs) on the basis of fuzzy evaluation of the probability that the evaluated nation will continue peaceful activities. It is shown that by determining the presence probability of undetected nuclear proliferating activities, nations under IS can be maintained at acceptably low proliferation risk levels even if the detection probability of current IS is decreased by dozens of percentage from the present value. This makes it possible to reduce inspection frequency and the number of collected samples, allowing the IAEA to cut costs per nation. This will contribute to further promotion and application of IS to more nations by the IAEA, and more efficient utilization of IAEA resources from the viewpoint of whole IS framework

  7. Analysis on the Decision Making on Nuclear Material Measurements for Safeguards Efficiency and Effectiveness on Research and Power Reactors

    International Nuclear Information System (INIS)

    Petit Wiringgalih; Basuki Wibowo

    2004-01-01

    This paper discusses three options in quantifying nuclear materials, ie. status quo, mobile unit measurement and centralised measurement. These three options are compared based on their safeguards effectiveness, timeline / efficiency, cost and safety aspects. Status quo measurement relies unverified estimation on nuclear materials, mobile unit measurement utilise NDA technique in order to obtain more accurate result while centralised measurement system transfers all measurements to another facility which posses more sophisticated equipment. Each of these three measurement options posesses relative advantages and disadvantages over the others. Status quo quantification is easy to perform but entails high inaccuracy. Mobile unit quantification is probably the best option, but it yields higher cost and radiation doses to workers. Centralised measurement facility is difficult to realise since the radioactive materials have to be coverted to their stable form. (author)

  8. Presence of renewable sources of energy, cogeneration, energy efficiency and distributed generation in the International Nuclear Information System (INIS)

    International Nuclear Information System (INIS)

    Pares Ferrer, Marianela; Oviedo Rivero, Irayda; Gonzalez Garcia, Alejandro

    2011-01-01

    The International Nuclear Information System (INIS) it was created in 1970 by the International Atomic Energy Agency (OIEA) with the objective of propitiating the exchange of scientific information and technique on the peaceful uses of the energy atomic. INIS processes most of scientific literature and technique in engineering matters nuclear, safeguard and non proliferation and applications in agriculture and health that it generates in the world and it contributes to create a repository of nuclear information for present and future generations. Additionally it includes economic aspects and environmental of other energy sources that facilitate comparative studies for the taking of decisions. The database INIS, is its main informative product and it counts with more than 3 million registrations. One of the services that lends the Center of Administration of the Information and Development of the Energy (CUBAENERGIA), like center INIS in Cuba, is the search of information on the peaceful use of the science and nuclear technology in the Countries Members and the registration of information on their applications in Cuba. More recently, it extends this service to the Renewable Sources application of Energy in the country; as part of the works of administration of the information that it carries out for the National Group of Renewable Energy, Cogeneration, Saving and Energy Efficiency, created in the 2007 and coordinated by the MINBAS with the participation of institutions belonging to Organisms of the Administration Central of the State. In this work the results of a preliminary study are presented on the witnesses in the INIS of the Renewable Sources of Energy, the Cogeneration, Energy Efficiency, and the Distributed Generation. As well as of the application of metric tools to the opposing registrations for the case of the Distributed generation, that which allowed to characterize their historical evolution, the participation for countries in their development and

  9. Nuclear techniques as efficient methods in entomology and pest management: potential and perspectives

    International Nuclear Information System (INIS)

    Seth, R.K.; Sethi, G.R.

    1996-01-01

    Major economic loss to crop and other agricultural commodities occurs due to insects, which has been a serious concern to entomologists. In this context, the possible uses and perspectives of the utilization of nuclear energy in entomological research studies and pest control tactics have been highlighted. (author). 100 refs., 1 fig

  10. The declaration regime: An efficient tool to improve control and protection of nuclear materials in France

    International Nuclear Information System (INIS)

    Pillette-Cousin, L.

    2001-01-01

    Full text: The French Government set up a national safeguards system under the authority of the Ministry for Industry to control nuclear materials within national boundaries and to ensure physical protection for nuclear materials, even for the small quantities held by users in industrial, medical and research areas. The main nuclear materials detained by small owners are depleted uranium and thorium. These materials are present in manufactured equipment (radiation shielding in industrial gammagraphy and radiotherapy, collimation devices and other accessories) which are used or unused, which may be damaged or left as scraps. The French protection and control system of nuclear materials is an original system based on detailed and comprehensive regulations, taking into account in a specific way the small users of nuclear materials. The decree no. 81-512 of 12 May 1981 establishes three different regimes: licensing, declaration and exemption, according to the nature and quantity of nuclear materials involved. Typically, the declaration regime applies to quantities of depleted uranium or thorium, greater than 1 kg and lower than 500 kg. The Order of 14 March 1984 sets the requirements related to the control and physical protection of nuclear materials in the frame of the declaration regime. A declaration must be established every year by the operator and sent to the IPSN, acting as technical support body of the national authority. This declaration provides the stock of all nuclear materials held by the operator and stock variations occurred during the previous year, including the identification of senders and receivers. Before fulfilling its annual declaration, the operator must carry out a physical inventory of all nuclear material, both used and unused. The declaration also describes the main features concerning facility layout related to surveillance and physical protection of materials. With respect to physical protection requirements, nuclear materials should be

  11. Respiration and nitrogen assimilation: targeting mitochondria-associated metabolism as a means to enhance nitrogen use efficiency.

    Science.gov (United States)

    Foyer, Christine H; Noctor, Graham; Hodges, Michael

    2011-02-01

    Considerable advances in our understanding of the control of mitochondrial metabolism and its interactions with nitrogen metabolism and associated carbon/nitrogen interactions have occurred in recent years, particularly highlighting important roles in cellular redox homeostasis. The tricarboxylic acid (TCA) cycle is a central metabolic hub for the interacting pathways of respiration, nitrogen assimilation, and photorespiration, with components that show considerable flexibility in relation to adaptations to the different functions of mitochondria in photosynthetic and non-photosynthetic cells. By comparison, the operation of the oxidative pentose phosphate pathway appears to represent a significant limitation to nitrogen assimilation in non-photosynthetic tissues. Valuable new insights have been gained concerning the roles of the different enzymes involved in the production of 2-oxoglutarate (2-OG) for ammonia assimilation, yielding an improved understanding of the crucial role of cellular energy balance as a broker of co-ordinate regulation. Taken together with new information on the mechanisms that co-ordinate the expression of genes involved in organellar functions, including energy metabolism, and the potential for exploiting the existing flexibility for NAD(P)H utilization in the respiratory electron transport chain to drive nitrogen assimilation, the evidence that mitochondrial metabolism and machinery are potential novel targets for the enhancement of nitrogen use efficiency (NUE) is explored.

  12. Ultrasmall cationic superparamagnetic iron oxide nanoparticles as nontoxic and efficient MRI contrast agent and magnetic-targeting tool

    Science.gov (United States)

    Uchiyama, Mayara Klimuk; Toma, Sergio Hiroshi; Rodrigues, Stephen Fernandes; Shimada, Ana Lucia Borges; Loiola, Rodrigo Azevedo; Cervantes Rodríguez, Hernán Joel; Oliveira, Pedro Vitoriano; Luz, Maciel Santos; Rabbani, Said Rahnamaye; Toma, Henrique Eisi; Poliselli Farsky, Sandra Helena; Araki, Koiti

    2015-01-01

    Fully dispersible, cationic ultrasmall (7 nm diameter) superparamagnetic iron oxide nanoparticles, exhibiting high relaxivity (178 mM−1s−1 in 0.47 T) and no acute or subchronic toxicity in Wistar rats, were studied and their suitability as contrast agents for magnetic resonance imaging and material for development of new diagnostic and treatment tools demonstrated. After intravenous injection (10 mg/kg body weight), they circulated throughout the vascular system causing no microhemorrhage or thrombus, neither inflammatory processes at the mesentery vascular bed and hepatic sinusoids (leukocyte rolling, adhesion, or migration as evaluated by intravital microscopy), but having been spontaneously concentrated in the liver, spleen, and kidneys, they caused strong negative contrast. The nanoparticles are cleared from kidneys and bladder in few days, whereas the complete elimination from liver and spleen occurred only after 4 weeks. Ex vivo studies demonstrated that cationic ultrasmall superparamagnetic iron oxide nanoparticles caused no effects on hepatic and renal enzymes dosage as well as on leukocyte count. In addition, they were readily concentrated in rat thigh by a magnet showing its potential as magnetically targeted carriers of therapeutic and diagnostic agents. Summarizing, cationic ultrasmall superparamagnetic iron oxide nanoparticles are nontoxic and efficient magnetic resonance imaging contrast agents useful as platform for the development of new materials for application in theranostics. PMID:26251595

  13. High Performance Computing and Storage Requirements for Nuclear Physics: Target 2017

    Energy Technology Data Exchange (ETDEWEB)

    Gerber, Richard [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Wasserman, Harvey [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2014-04-30

    In April 2014, NERSC, ASCR, and the DOE Office of Nuclear Physics (NP) held a review to characterize high performance computing (HPC) and storage requirements for NP research through 2017. This review is the 12th in a series of reviews held by NERSC and Office of Science program offices that began in 2009. It is the second for NP, and the final in the second round of reviews that covered the six Office of Science program offices. This report is the result of that review

  14. The environment - Can Britain meet its environmental targets without nuclear energy electricity?

    International Nuclear Information System (INIS)

    Kay, Martin

    1995-01-01

    The video is one section of a four module package produced as part of a 'Presenter's Toolkit' for the British Government's Nuclear Review during 1994. The video has been produced to supplement face to face meetings in such a way that it is thought provoking. It actively seeks to engage minds by posing questions which are then discussed as fully as the audience requires. This novel approach has proved demanding, but when used in conjunction with the full toolkit, (which includes notes, audio tapes and brochures) it has proved an effective way to communicate with small groups of key players and opinion formers

  15. Towards novel efficient and stable nuclear import signals: synthesis and properties of trimethylguanosine cap analogs modified within the 5',5'-triphosphate bridge.

    Science.gov (United States)

    Zytek, Malgorzata; Kowalska, Joanna; Lukaszewicz, Maciej; Wojtczak, Blazej A; Zuberek, Joanna; Ferenc-Mrozek, Aleksandra; Darzynkiewicz, Edward; Niedzwiecka, Anna; Jemielity, Jacek

    2014-12-07

    A trimethylguanosine (TMG) cap is present at the 5' end of several small nuclear and nucleolar RNAs. Recently, it has been reported that the TMG cap is a potential nuclear import signal for nucleus-targeting therapeutic nucleic acids and proteins. The import is mediated by recognition of the TMG cap by the snRNA transporting protein, snurportin1. This work describes the synthesis and properties of a series of dinucleotide TMG cap (m3(2,2,7)GpppG) analogs modified in the 5',5'-triphosphate bridge as tools to study TMG cap-dependent biological processes. The bridge was altered at different positions by introducing either bridging (imidodiphosphate, O to NH and methylenebisphosphonate, O to CH2) or non-bridging (phosphorothioate, O to S and boranophosphate, O to BH3) modifications, or by elongation to tetraphosphate. The stability of novel analogs in blood serum was studied to reveal that the α,β-bridging O to NH substitution (m3(2,2,7)GppNHpG) confers the highest resistance. Short RNAs capped with analogs containing α,β-bridging (m3(2,2,7)GppNHpG) or β-non-bridging (m3(2,2,7)GppSpG D2) modifications were resistant to decapping pyrophosphatase, hNudt16. Preliminary studies on binding by human snurportin1 revealed that both O to NH and O to S substitutions support this binding. Due to favorable properties in all three assays, m3(2,2,7)GppNHpG was selected as a promising candidate for further studies on the efficiency of the TMG cap as a nuclear import signal.

  16. Syringe shape and positioning relative to efficiency volume inside dose calibrators and its role in nuclear medicine quality assurance programs

    Energy Technology Data Exchange (ETDEWEB)

    Santos, J.A.M. [Servico de Fisica Medica, Instituto Portugues de Oncologia do Porto Francisco Gentil, E.P.E., Rua Dr. Antonio Bernardino de Almeida, 4200072 Porto (Portugal); Centro de Investigacao, Instituto Portugues de Oncologia do Porto Francisco Gentil, E.P.E., Rua Dr. Antonio Bernardino de Almeida, 4200072 Porto (Portugal)], E-mail: a.miranda@portugalmail.pt; Carrasco, M.F. [Servico de Fisica Medica, Instituto Portugues de Oncologia do Porto Francisco Gentil, E.P.E., Rua Dr. Antonio Bernardino de Almeida, 4200072 Porto (Portugal); Centro de Investigacao, Instituto Portugues de Oncologia do Porto Francisco Gentil, E.P.E., Rua Dr. Antonio Bernardino de Almeida, 4200072 Porto (Portugal); Lencart, J. [Servico de Fisica Medica, Instituto Portugues de Oncologia do Porto Francisco Gentil, E.P.E., Rua Dr. Antonio Bernardino de Almeida, 4200072 Porto (Portugal); Bastos, A.L. [Servico de Medicina Nuclear, Instituto Portugues de Oncologia do Porto Francisco Gentil, E.P.E., Rua Dr. Antonio Bernardino de Almeida, 4200072 Porto (Portugal)

    2009-06-15

    A careful analysis of geometry and source positioning influence in the activity measurement outcome of a nuclear medicine dose calibrator is presented for {sup 99m}Tc. The implementation of a quasi-point source apparent activity curve measurement is proposed for an accurate correction of the activity inside several syringes, and compared with a theoretical geometric efficiency model. Additionally, new geometrical parameters are proposed to test and verify the correct positioning of the syringes as part of acceptance testing and quality control procedures.

  17. Mouse embryonic stem cells efficiently lipofected with nuclear localization peptide result in a high yield of chimeric mice and retain germline transmission potency.

    Science.gov (United States)

    Ma, Haiching; Liu, Qin; Diamond, Scott L; Pierce, Eric A

    2004-06-01

    Embryonic stem (ES) cells are an important tool in developmental biology, genomics, and transgenic methods, as well as in potential clinical applications such as gene therapy or tissue engineering. Electroporation is the standard transfection method for mouse ES (mES) cells because lipofection is quite inefficient. It is also unclear if mES cells treated with cationic lipids maintain pluripotency. We have developed a simple lipofection method for high efficiency transfection and stable transgene expression by employing the nonclassical nuclear localization signal M9 derived from the heterogeneous nuclear ribonucleoprotein A1. In contrast to using 20 microg DNA for 10 x 10(6) cells via electroporation which resulted in 10-20 positive cells/mm2, M9-assisted lipofection of 2 x 10(5) cells with 2 microg DNA resulted in > 150 positive cells/mm2. Electroporation produced only 0.16% EGFP positive cells with fluorescence intensity (FI) > 1000 by FACS assay, while M9-lipofection produced 36-fold more highly EGFP positive cells (5.75%) with FI > 1000. Using 2.5 x 10(6) ES cells and 6 microg linearized DNA followed by selection with G418, electroporation yielded 17 EGFP expressing colonies, while M9-assisted lipofection yielded 72 EGFP expressing colonies. The mES cells that stably expressed EGFP following M9-assisted lipofection yielded > 66% chimeric mice (8 of 12) and contributed efficiently to the germline. In an example of gene targeting, a knock-in mouse was produced from an ES clone screened from 200 G418-resistant colonies generated via M9-assisted lipofection. To our knowledge, this is the first report of generation of transgenic or knock-in mice obtained from lipofected mES cells and this method may facilitate large scale genomic studies of ES developmental biology or large scale generation of mouse models of human disease. Copyright 2003 Elsevier Inc.

  18. Effective and efficient software development method for real time safety systems for nuclear power plants

    International Nuclear Information System (INIS)

    Manoj, P.; Parimalam, P.; Shanmugam, A.; Murali, N.

    2013-01-01

    The objective of this paper is to present the effective and efficient methods for developing application software for Distributed Real Time Systems for Prototype Fast Breeder Reactor. It discusses the effective ways to reduce the language and syntax errors while capturing the requirements. This paper suggests an efficient way of requirements capture and coding application software for I and C systems so that the quality factors of the software such as reliability, maintainability and testability are improved. (author)

  19. Membrane and Nuclear Permeabilization by Polymeric pDNA Vehicles: Efficient Method for Gene Delivery or Mechanism of Cytotoxicity?

    Science.gov (United States)

    Grandinetti, Giovanna; Smith, Adam E.; Reineke, Theresa M.

    2012-01-01

    The aim of this study is to compare the cytotoxicity mechanisms of linear PEI to two analogous polymers synthesized by our group: a hydroxyl-containing poly(L-tartaramidoamine) (T4) and a version containing an alkyl chain spacer poly(adipamidopentaethylenetetramine) (A4) by studying the cellular responses to polymer transfection. We have also synthesized analogues of T4 with different molecular weights (degrees of polymerization of 6, 12, and 43) to examine the role of molecular weight on the cytotoxicity mechanisms. Several mechanisms of polymer-induced cytotoxicity are investigated, including plasma membrane permeabilization, the formation of potentially harmful polymer degradation products during transfection including reactive oxygen species, and nuclear membrane permeabilization. We hypothesized that since cationic polymers are capable of disrupting the plasma membrane, they may also be capable of disrupting the nuclear envelope, which could be a potential mechanism of how the pDNA is delivered into the nucleus (other than nuclear envelope breakdown during mitosis). Using flow cytometry and confocal microscopy, we show that the polycations with the highest amount of protein expression and toxicity, PEI and T443, are capable of inducing nuclear membrane permeability. This finding is important for the field of nucleic acid delivery in that not only could direct nucleus permeabilization be a mechanism for pDNA nuclear import but also a potential mechanism of cytotoxicity and cell death. We also show that the production of reactive oxygen species is not a main mechanism of cytotoxicity, and that the presence or absence of hydroxyl groups as well as polymer length plays a role in polyplex size and charge in addition to protein expression efficiency and toxicity. PMID:22175236

  20. Probing the exotic structure of {sup 8}B by its elastic scattering and breakup reaction on nuclear targets

    Energy Technology Data Exchange (ETDEWEB)

    Lukyanov, V.K.; Zemlyanaya, E.V.; Lukyanov, K.V. [Joint Institute for Nuclear Research, Dubna (Russian Federation); Kadrev, D.N.; Antonov, A.N.; Gaidarov, M.K. [Bulgarian Academy of Sciences, Institute for Nuclear Research and Nuclear Energy, Sofia (Bulgaria); Spasova, K. [Bulgarian Academy of Sciences, Institute for Nuclear Research and Nuclear Energy, Sofia (Bulgaria); University ' ' Ep. K. Preslavski' ' , Shumen (Bulgaria)

    2017-02-15

    The structure of the exotic {sup 8}B nucleus is studied by means of elastic scattering, as well as its breakup on nuclear targets. We present microscopic calculations of the optical potentials (OPs) and cross sections of elastic scattering of {sup 8}B on {sup 12}C, {sup 58}Ni, and {sup 208}Pb targets at energies 20 < E < 170 MeV. The density distributions of {sup 8}B obtained within the variational Monte Carlo (VMC) model and the three-cluster model (3CM) are used to construct the potentials. The real part of the hybrid OP is calculated using the folding model with the direct and exchange terms included, while the imaginary part is obtained on the base of the high-energy approximation (HEA) and also taken to be equal to the microscopic real part of the OP. In this model the only free parameters are the depths of the real and imaginary parts of OP obtained by fitting the elastic scattering experimental data. A dependence of their values on the model density of {sup 8} B is found. In addition, the cluster model, in which {sup 8}B consists of a p-halo and the {sup 7} Be core, is applied to calculate the breakup cross sections of the {sup 8}B nucleus on {sup 9}Be, {sup 12}C, and {sup 197}Au targets, as well as momentum distributions of {sup 7}Be fragments, and a comparison with the existing experimental data is made. (orig.)