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Sample records for dvunitevykh razryvov dnk

  1. DNK Energy Day '98. Energy and environment as subjects in the dialog between politics, economy and the scientific community; DNK Energietag '98. Energie und Umwelt im Dialog zwischen Politik, Wirtschaft und Wissenschaft

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-07-01

    This fifth one-day energy forum of the DNK held in Bonn on 26 November 1998 once again gathered many representatives of industry, science and research, and the political arena. The date of the meeting in mid-November was well-chosen, as it offered the opportunity to discuss the outcome of the 17th World Energy Congress held two months ago in Houston/USA, and to communicate on expectations in Germany linked to the future energy policy of the new coalition in power. The general theme of the forum, 'Energy and environment as issues of a dialogue of politicians, industry and science' thus fits in well with the global energy issues. The contributions to the panel discussion with the concluding summary of Dr. Ott as well as the lectures presented at the meeting are reproduced or this conference report from the manuscripts and the minutes. (orig./CB) [German] Der 5. Energietag des DNK in Bonn am 26. November 1998 hat erneut zahlreiche Repraesentanten aus Wirtschaft, Wissenschaft und Politik zusammengefuehrt. Der Zeitpunkt Mitte November 1998 bot die Gelegenheit, einerseits die Ergebnisse des 17. Kongresses des Weltenergierates, der gerade 2 Monate zuvor in Houston stattfand, zu erlaeutern und zum anderen, ueber die energiepolitischen Erwartungen an die neue Bundesregierung und deren Vorstellungen zur kuenftigen Energiepolitik zu diskutieren. Das Generalthema des Forums 'Energie und Umwelt im Dialog zwischen Politik, Wirtschaft und Wissenschaft' hatte damit aktuellen Bezug. Die Beitraege der Podiumsdiskussion mit den Schlussbemerkungen von Dr. Ott sind wie die Vortraege weitgehend im Originalwortlaut wiedergegeben. Sie werden im vorliegenden Heft dokumentiert. (orig./RHM)

  2. Immunohistochemical distribution of myotonic dystrophy kinase (DNK) in muscle

    Energy Technology Data Exchange (ETDEWEB)

    Whiting, E.J.; Tamai, K. [Univ. of Ottawa (Canada); Waring, J.D. [Chilrdren`s Hospital of Eastern Ontario, Ottawa (Canada)] [and others

    1994-09-01

    Myotonic dystrophy (DM) is the most common form of inherited neuromuscular disease in adults and is characterized by progressive muscle wasting and myotonia. The mutation responsible for DM has been identified as the expansion of a polymorphic (CTG)n repeat in the 3{prime} untranslated region of a gene encoding a putative serine/threonine kinase (DMK). We have raised a polyclonal raised a polyclonal rabbit antisera against a fusion protein encoding exons 11-15 of DMK. The antisera detects both the full length and a truncated isoform (missing amino acids corresponding to exons 13-15) of the human DMK expressed in a recombinant baculovirus system. In addition, it recognizes a 69 kDA protein on Western blots of both human and mouse myoblasts. Use of this antiserum in immunohistochemical studies of human tissue demonstrates that DMK is expressed in the cytoplasm of both skeletal and smooth muscle and is expressed postsynaptically (as determined by codistribution with acetylcholinesterase and acetylcholine receptors) within the vicinity of neuromuscular junction of skeletal muscle. Further, no obvious differences in DMK localization were observed between muscle tissues from normal and DM-affected individuals.

  3. Persistence of decidual NK cells and KIR genotypes in healthy pregnant and preeclamptic women: a case-control study in the third trimester of gestation

    Directory of Open Access Journals (Sweden)

    Cerbón Marco

    2011-01-01

    Full Text Available Abstract Background Natural Killer (NK cells are the most abundant lymphocytes in the decidua during early gestation. The interactions of NK cells with the extravillous cytotrophoblast have been associated with a normal spiral artery remodeling process, an essential event for a successful pregnancy. Recent data indicate that alterations in the amount of decidual NK (dNK cells contribute to the development of preeclampsia (PE. Moreover, genetic studies suggest that Killer Immunoglobulin-like Receptors (KIR expressed in dNK cells influence the susceptibility to PE. Although dNK cells have been well characterized during early pregnancy, they have been scarcely studied in the third trimester of gestation. The aim of this work was to characterize dNK cells at the last trimester of gestation and to analyze the KIR genotype of healthy and PE women. Methods Decidual samples were obtained during Caesarean section from control (n = 10 and PE (n = 9 women. Flow cytometric analysis of CD3, CD56, CD16 and CD9 was used to characterize and quantify dNK cells in both groups. Cell surface markers from decidual leukocytes were compared with PBMC from healthy donors. KIR genotyping was performed in genomic DNA (control, n = 86; PE, n = 90 using PCR-SSP. Results The results indicate that dNK cells persist throughout pregnancy. They represented 20% of total leukocytes in control and PE groups, and they expressed the same cell surface markers (CD3-, CD56+, CD16- and CD9+ as dNK in the first trimester of gestation. There were no significant differences in the percentage of dNK cells between control and PE groups. The analysis of KIR gene frequencies and genotypes was not statistically different between control and PE groups. The ratio of activating to inhibitory genes indicated that the overall inhibitory balance (0.2-0.5 was more frequent in the PE group (control, 31.3% vs PE, 45.5%, and the activating balance (0.6-1.1 was more frequent in the control group (control

  4. In vitro and in vivo anthelmintic activity of (-)-6,6'-dinitrohinokinin against schistosomula and juvenile and adult worms of Schistosoma mansoni.

    Science.gov (United States)

    Pereira, Ana C; Silva, Márcio L A E; Souza, Julia Medeiros; Laurentiz, Rosangela S de; Rodrigues, Vanderlei; Januário, Ana H; Pauletti, Patrícia M; Tavares, Denise C; Filho, Ademar A Da Silva; Cunha, Wilson R; Bastos, Jairo K; Magalhães, Lizandra G

    2015-09-01

    The chemotherapy of schistosomiasis relies on the use of praziquantel. However, concerns over drug resistance have encouraged the search for new drug leads. This paper is the first report on the in vitro and in vivo activity of (-)-6,6'-dinitrohinokinin (DNK) against Schistosoma mansoni. In vitro, the lethal concentrations for 50% of parasites (LC50) of DNK against adult worms were 103.9±3.6 and 102.5±4.8μM at 24 and 72h, respectively. Scanning electron microscopy images showed extensive tegumental alterations such as peeling and smaller numbers of tubercles in the spine of adult worms. DNK also elicited high mortality of schistosomula, with LC50 values of 57.4±2.3, 32.5±0.9, and 20.4±1.2μM at 24, 48, and 72h, respectively. DNK displayed moderate activity against the juvenile liver parasite, with an LC50 value of 179.5±2.3 μM at 72h. This compound reduced the total number of eggs by over 83%, and it affected the development of eggs produced by adult worms. The selectivity index showed that at 24h, DNK was 8.5 and 15.4 times more toxic to the adult worms and schistosomula than to Chinese hamster lung fibroblast cells, respectively. Treatment of infected mice with DNK moderately decreased worm burden (33.8-52.3%), egg production (40.7-60.0%), and spleen and liver weights. Together, our results indicated that DNK presents moderate in vitro and in vivo activities against S. mansoni, and it might therefore be interesting to explore the structure-activity relationship of the antischistosomal activity of this compound.

  5. Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase

    DEFF Research Database (Denmark)

    Mikkelsen, Niels Egil; Munch-Petersen, Birgitte; Eklund, Hans

    2008-01-01

    The Drosophila Melanogaster multisubstrate deoxyribonucleoside kinase (dNK) has a high turnover rate and a wide substrate range that makes it a very good candidate for gene therapy. This concept is based on introducing a suicide gene into malignant cells in order to activate a pro...

  6. Expression of Interferon γ by Decidual Cells and Natural Killer Cells at the Human Implantation Site: Implications for Preeclampsia, Spontaneous Abortion, and Intrauterine Growth Restriction.

    Science.gov (United States)

    Chen, Chie-Pein; Piao, Longzhu; Chen, Xilin; Yu, Jianhua; Masch, Rachel; Schatz, Frederick; Lockwood, Charles J; Huang, S Joseph

    2015-11-01

    Human first-trimester decidual cells (FTDCs) chemoattract CXCR3-expressing circulating CD56(bright)CD16(-) natural killer (NK) cells, which increase uteroplacental blood flow by remodeling spiral arteries and arterioles. This recruitment reflects elevated FTDC expression of NK cell-recruiting induced protein 10 and interferon (IFN)-inducible T-cell-α chemoattractant produced in response to the synergistic effects of tumor necrosis factor α (TNF-α) and IFN-γ stimulation. Decidual macrophages express TNF-α, whereas the cellular origin of IFN-γ is unclear. Therefore, this study aims to identify the cell source(s) of IFN-γ in human first trimester decidua. Immunostaining of decidual sections revealed that both FTDCs and decidual NK (dNK) cells express IFN-γ. Although individual dNK cells express higher IFN-γ levels, the more numerous FTDCs account for greater proportion of total IFN-γ immunostaining. Freshly isolated FTDCs express greater IFN-γ staining than dNK cells as measured by flow cytometry, whereas incubation of dNK cells with documented NK cell activators significantly increases IFN-γ above FTDC levels. Confluent FTDCs intrinsically produce, but paradoxically respond to, exogenous IFN-γ.

  7. Animal deoxyribonucleoside kinases: 'forward' and 'retrograde' evolution of their substrate specificity

    DEFF Research Database (Denmark)

    Piskur, Jure; Sandrini, Michael; Knecht, Wolfgang;

    2004-01-01

    specificity. Recent studies suggest that in the animal lineage one of the brogenitor kinases, the so-called dCK/dGK/TK2-like gene, was duplicated prior to separation of the insect and mammalian lineages. Thereafter, insects lost all but one kinase, dNK (EC 2.7.1.145), which subsequently, through remodelling...

  8. Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases

    DEFF Research Database (Denmark)

    Sandrini, Michael; Soderbom, F.; Mikkelsen, N.E.;

    2007-01-01

    The salvage of deoxyribonucleosides in the social amoeba Dictyostelium discoideum, which has an extremely A + T-rich genome, was investigated. All native deoxyribonucleosides were phosphorylated by D. discoideum cell extracts and we subcloned three deoxyribonucleoside kinase (dNK) encoding genes....

  9. Data of evolutionary structure change: 1CERD-3CIFA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CERD-3CIFA 1CER 3CIF D A -MKVGINGFGRIGRQVFRILHSRG-VEVALINDLT-DNK...322 VAL CA 435 ALA CA 392 LEU CA 460 3CIF... A 3CIFA 3CIF A 3CIFA IND...ne>ARG CA 260 ARG CA 299 ALA CA 243 3CIF

  10. Data of evolutionary structure change: 1CERO-3CIFB [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CERO-3CIFB 1CER 3CIF O B -MKVGINGFGRIGRQVFRILHSRG-VEVALINDLT-DNK...line> ALA CA 375 LEU CA 455 3CIF ...B 3CIFB CMERNDITVVALEU CA 389 3CIF B 3CIF...94 ARG CA 258 ARG CA 299 ALA CA 245 3CIF

  11. Data of evolutionary structure change: 1CERR-3CIFC [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CERR-3CIFC 1CER 3CIF R C -MKVGINGFGRIGRQVFRILHSRG-VEVALINDLT-DNK...A 322 VAL CA 435 ALA CA 392 LEU CA 459 3CIF... C 3CIFC LYS CA 257 THR CA 269 LEU CA 387 3CIF... C 3CIFC INDPFMDVEYM<

  12. Data of evolutionary structure change: 1CERQ-3CIFA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CERQ-3CIFA 1CER 3CIF Q A -MKVGINGFGRIGRQVFRILHSRG-VEVALINDLT-DNK... 326 VAL CA 439 ALA CA 385 LEU CA 456 3CIF... A 3CIFA LYS CA 257 THR CA 272 LEU CA 392 3CIF... A 3CIFA INDPFMDVE

  13. Data of evolutionary structure change: 1CERR-3CIFB [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CERR-3CIFB 1CER 3CIF R B -MKVGINGFGRIGRQVFRILHSRG-VEVALINDLT-DNK... VAL CA 435 ALA CA 392 LEU CA 459 3CIF... B 3CIFB LYS CA 257 THR CA 269 LEU CA 387 3CIF... B 3CIFB INDPFMDVEYM

  14. Data of evolutionary structure change: 1CERQ-3CIFB [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CERQ-3CIFB 1CER 3CIF Q B -MKVGINGFGRIGRQVFRILHSRG-VEVALINDLT-DNK... 326 VAL CA 439 ALA CA 385 LEU CA 456 3CIF... B 3CIFB LYS CA 257 THR CA 272 LEU CA 392 3CIF... B 3CIFB INDPFMDVEYM

  15. Data of evolutionary structure change: 1CERR-3CIFA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CERR-3CIFA 1CER 3CIF R A -MKVGINGFGRIGRQVFRILHSRG-VEVALINDLT-DNK... VAL CA 435 ALA CA 392 LEU CA 459 3CIF... A 3CIFALYS CA 257 THR CA 269 LEU CA 387 3CIF... A 3CIFA INDPFMDVEYM

  16. Broadband excitation in solid-state NMR using interleaved DANTE pulse trains with N pulses per rotor period.

    Science.gov (United States)

    Lu, Xingyu; Trébosc, Julien; Lafon, Olivier; Carnevale, Diego; Ulzega, Simone; Bodenhausen, Geoffrey; Amoureux, Jean-Paul

    2013-11-01

    We analyze the direct excitation of wide one-dimensional spectra of nuclei with spin I=1/2 or 1 in rotating solids submitted to pulse trains in the manner of Delays Alternating with Nutations for Tailored Excitation (DANTE), either with one short rotor-synchronized pulse of duration τp in each of K rotor periods (D1(K)) or with N interleaved equally spaced pulses τp in each rotor period, globally also extending over K rotor periods (D(N)(K)). The excitation profile of D(N)(K) scheme is a comb of rf-spikelets with Nν(R)=N/T(R) spacing from the carrier frequency, and a width of each spikelet inversely proportional to the length, KT(R), of D(N)(K) scheme. Since the individual pulse lengths, τp, are typically of a few hundreds of ns, D(N)(K) scheme can readily excite spinning sidebands families covering several MHz, provided the rf carrier frequency is close enough to the resonance frequency of one the spinning sidebands. If the difference of isotropic chemical shifts between distinct chemical sites is less than about 1.35/(KT(R)), D(N)(K) scheme can excite the spinning sidebands families of several sites. For nuclei with I=1/2, if the homogeneous and inhomogeneous decays of coherences during the DANTE sequence are neglected, the K pulses of a D1(K) train have a linearly cumulative effect, so that the total nutation angle is θ(tot)=K2πν1τp, where ν1 is the rf-field amplitude. This allows obtaining nearly ideal 90° pulses for excitation or 180° rotations for inversion and refocusing across wide MAS spectra comprising many spinning sidebands. If one uses interleaved DANTE trains D(N)(K) with N>1, only spinning sidebands separated by intervals of Nν(R) with respect to the carrier frequency are observed as if the effective spinning speed was Nν(R). The other sidebands have vanishing intensities because of the cancellation of the N contributions with opposite signs. However, the intensities of the remaining sidebands obey the same rules as in spectra obtained

  17. Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation.

    Directory of Open Access Journals (Sweden)

    Marion eDuriez

    2014-07-01

    Full Text Available Maternofetal pathogen transmission is partially controlled at the level of the maternal uterine mucosa at the fetal implantation site (the decidua basalis, where maternal and fetal cells are in close contact. Toll-like receptors (TLRs may play an important role in initiating rapid immune responses against pathogens in the decidua basalis, however the tolerant microenvironment should be preserved in order to allow fetal development. Here we investigated the expression and functionality of TLRs expressed by decidual macrophages (dMs and NK cells (dNKs, the major decidual immune cell populations.We report for the first time that both human dMs and dNK cells express mRNAs encoding TLRs 1-9, albeit with a higher expression level in dMs. TLR2, TLR3 and TLR4 protein expression checked by flow cytometry was positive for both dMs and dNK cells. In vitro treatment of primary dMs and dNK cells with specific TLR2, TLR3, TLR4, TLR7/8 and TLR9 agonists enhanced their secretion of pro- and anti-inflammatory cytokines, as well as cytokines and chemokines involved in immune cell crosstalk. Only dNK cells released IFN-γ, whereas only dMs released IL-1β, IL-10 and IL-12. TLR9 activation of dMs resulted in a distinct pattern of cytokine expression compared to the other TLRs. The cytokine profiles expressed by dMs and dNK cells upon TLR activation are compatible with maintenance of the fetotolerant immune environment during initiation of immune responses to pathogens at the maternofetal interface.

  18. Remedial Investigation Badger Army Ammunition Plant, Baraboo, Wisconsin. Volume 2. Appendices D.2 Through F

    Science.gov (United States)

    1991-01-01

    Well Number DNK Wed Numoer TypeofWell WafTableOtMrvanonWeHlElU LAeca Locauon Dat Wenl Instaled Pistanc Wel Is N/4 of l/4 of Seco _.._.., m -" V v D. R...Perons tamne Firm) Distance wellIs Frina WastedSource boundary Tel Intale Rr - Peso W N!T p.d / Is We.I A Point or Encorcemam StUL Application! a U cim

  19. Ekspresija antioksidativnih enzima i transkripcionog faktora Nrf2 kod pacijentkinja sa benigno, premaligno i maligno transformisanim endometrijumom

    OpenAIRE

    Todorović, Ana U.

    2013-01-01

    Tokom čitavog životnog ciklusa aerobni organizmi su izloženi brojnim endogenim i egzogenim faktorima koji indukuju povećanje produkcije ROS-a. Prisutni u fiziološkim koncentracijama, reaktivni molekuli ROS-a imaju značajnu ulogu u važnim ćelijskim procesima kao što su regulacija signalnih kaskada i genske ekspresije. U visokim koncentracijama ROS mogu oksidovati ćelijske proteine, lipide i DNK i time dovesti do promena strukture i funkcije, oštećenja, pa i smrti ćelije. Kada koncentracija slo...

  20. Shal/K(v4 channels are required for maintaining excitability during repetitive firing and normal locomotion in Drosophila.

    Directory of Open Access Journals (Sweden)

    Yong Ping

    Full Text Available BACKGROUND: Rhythmic behaviors, such as walking and breathing, involve the coordinated activity of central pattern generators in the CNS, sensory feedback from the PNS, to motoneuron output to muscles. Unraveling the intrinsic electrical properties of these cellular components is essential to understanding this coordinated activity. Here, we examine the significance of the transient A-type K(+ current (I(A, encoded by the highly conserved Shal/K(v4 gene, in neuronal firing patterns and repetitive behaviors. While I(A is present in nearly all neurons across species, elimination of I(A has been complicated in mammals because of multiple genes underlying I(A, and/or electrical remodeling that occurs in response to affecting one gene. METHODOLOGY/PRINCIPAL FINDINGS: In Drosophila, the single Shal/K(v4 gene encodes the predominant I(A current in many neuronal cell bodies. Using a transgenically expressed dominant-negative subunit (DNK(v4, we show that I(A is completely eliminated from cell bodies, with no effect on other currents. Most notably, DNK(v4 neurons display multiple defects during prolonged stimuli. DNK(v4 neurons display shortened latency to firing, a lower threshold for repetitive firing, and a progressive decrement in AP amplitude to an adapted state. We record from identified motoneurons and show that Shal/K(v4 channels are similarly required for maintaining excitability during repetitive firing. We then examine larval crawling, and adult climbing and grooming, all behaviors that rely on repetitive firing. We show that all are defective in the absence of Shal/K(v4 function. Further, knock-out of Shal/K(v4 function specifically in motoneurons significantly affects the locomotion behaviors tested. CONCLUSIONS/SIGNIFICANCE: Based on our results, Shal/K(v4 channels regulate the initiation of firing, enable neurons to continuously fire throughout a prolonged stimulus, and also influence firing frequency. This study shows that Shal/K(v4

  1. ANALISIS BEBAN KERJA OPERATOR MESIN PEMOTONG BATU BESAR (SIRKEL 160 CM DENGAN MENGGUNAKAN METODE 10 DENYUT

    Directory of Open Access Journals (Sweden)

    Andriyanto Andriyanto

    2012-12-01

    Full Text Available PT. PAH merupakan sebuah industri pemotongan batu magma (magma stone yang terletak di jalan Magelang Km. 15 Yogyakarta. Di perusahaan pemotongan batu ini terdapat 3 ukuran pemotongan yaitu sirkel besar (diameter 160 cm, sirkel sedang (diameter 60 - 90 cm dan sirkel kecil (diameter 30 - 50 cm. Penelitian ini khususnya pada pemotongan batu besar, dimana terdapat keluhan kelelahan operator yang terdiri dari operator utama dan operator pembantu. Penelitian dilakukan dengan tujuan untuk mengetahui tingkat beban kerja fisik operator pemotong batu besar (sirkel 160 dengan metode 10 denyut. Dalam penelitian ini ditentukan lama waktu istirahat yang dibutuhkan operator utama maupun pembantu. Selanjutnya dilakukan implementasi waktu istirahat tersebut dan dilakukan pengukuran kembali untuk mengetahui penurunan denyut nadi kerja operator. Penerapan waktu istirahat tersebut membawa penurunan DNK operator masuk kategori ringan dan % CVL masuk kategori tidak terjadi kelelahan.

  2. Oxidative Stress, Skin Aging and Antioxidant Therapy / Oksidacioni Stres, Starenje Kože I Antioksidaciona Terapija

    Directory of Open Access Journals (Sweden)

    Stojiljković Dragana

    2014-12-01

    Full Text Available Starenje kože je dinamičan proces koji se dešava usled hronološkog starenja i foto-starenja izazvanog izlaganjem delovanju faktora spoljašnje sredine, pre svega UV (eng. ultraviolet zračenja. Kumulativni oksidacioni stres, formiranje slobodnih radikala i njihov nepovoljan uticaj na biološke sisteme u ćelijama kože, predstavljju čest i zajednički mehanizam oba procesa starenja kože. Formirane reaktivne kiseonične vrste (eng. reactive oxygen species-ROS mogu dovesti do oksidacije gradivnih molekula ćelija. Peroksidacija lipida, oštećenje membranskih proteina i DNK mutacije mogu dovesti do pojave strukturnih, funkcionalnih, kao i estetskih promena na koži, a takođe mogu pokrenuti razvoj mnogih bolesti.

  3. Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals

    Directory of Open Access Journals (Sweden)

    Radosavljević V.

    2014-01-01

    Full Text Available In this work there was evaluated the method of detection of methicillin resistant Staphylococcus aureus (MRSA by using two molecular and three phenotypic tests in investigation procedure of 70 strains of S.aureus isolated from animals. Recent findings of the new mecA homologue, mecALGA251, minimise the significance of mecA gene presence detection as a confirmation method of methicillin resistant Staphylococcus aureus identification. For this reason, along with multiplex PCR set of primers(165rDNK, nuc, mecA for detection mecA gene, there was also used multiplex PCR set of primers (spa, mecA, pvl, mecALGA251 for differentiation mecALGA251 from mecA, with simultaneous detection of luk-PV and spa gene fragments. In all 70 investigated isolates there was detected the presence of specific 16 SrDNK fragment and nuc gene which encodes a thermostable S. aureus nuclease, while in 5 out of 70 S. aureus isolates, there was proven mecA gene presence using two multiplex PCR tests. In the investigated strains there was determined neither mecC (mecALGA251gene presence, nor Panton Valentine Leukocidin encoding gene. By application cefoxitin disk-diffusion, latex-agglutination and two multiplex PCR tests, the identical results in identification 5 methicillin resistant out of 70 investigated S. aureus strains were obtained. In our investigation there was determined a complete correlation between the results of phenotypic and genotypic identification of methicillin resistant S. aureus. [Projekat Ministarstva nauke Republike Srbije, br. TR 31079

  4. Phenotype of decidual NK cells in women with hypertensive disorder complicating pregnancy%妊娠期高血压疾病患者子宫蜕膜NK细胞表型分析

    Institute of Scientific and Technical Information of China (English)

    周娟; 肖小敏

    2011-01-01

    目的:观察妊娠期高血压疾病患者子宫蜕膜自然杀伤细胞(dNK 细胞)的表型.方法:选取2008年8月至2009年3月在广州市暨南大学附属第一医院妇产科因妊娠期高血压疾病行剖宫产的单胎妊娠孕妇20例作为妊娠期高血压组,随机选取同期在此院因社会心理因素行选择性剖宫产的正常单胎妊娠孕妇15例作为正常对照组.收集孕晚期子宫蜕膜组织,机械研磨加梯度离心法提取蜕膜内单核细胞,流式细胞技术(FCM)筛选出dNK细胞,并检测dNK细胞表面CD56及CD16的表达情况.结果:(1)妊娠期高血压组与正常对照组CD56brightCD16-CD3-dNK细胞的数量均多于CD56dimCD16+CD3- dNK细胞,且差异显著(均P<0.01).(2)妊娠期高血压组与正常对照组CD56brightCD16-CD3- dNK细胞所占比例无显著差异(P>0.05),CD56dimCD16+CD3- dNK细胞所占比例亦无显著差异(P>0.05).(3)妊娠期高血压组与正常对照组dNK细胞表面CD16分子的表达率并无显著差异(P>0.05).结论:妊娠期高血压疾病患者与正常孕妇孕晚期子宫蜕膜内dNK细胞表型无明显改变,均以CD56brightCD16-CD3-亚型为主.%AIM : To investigate the phenotype of uterine decidual natural killer cells ( dNK cells ) in women with hypertensive disorder complicating pregnancy ( HDCP ).METHODS : All the study subjects were collected from Department of Ohstetrics and Gynecology, The First Affiliated Hospital of Jinan University, (;uangzhou, China.Twenty cases of singleton pregnancy who underwent caesarean section because of HDCP were selected as HDCP group, and 15 cases of singleton pregnancy received selective caesarean section because of social - psychological concerns were also randomly selected as normal control group.The decidual tissues were sampled immediately after caesarean section.The mononuclear cells were extracted from the tissues by means of mechanic grinding and gradient centrifugation.The technique of flow cytometry was used for dNK

  5. Characterization of Serratia fonticola, an opportunistic pathogen isolated from drinking water

    Directory of Open Access Journals (Sweden)

    Tasić S.

    2013-01-01

    Full Text Available We characterized the ST2 strain of Serratia fonticola isolated from drinking water of a capping spring on Mt. Vlasina. The ST2 strain isolated from bottled water showed the characteristics of Enterobacteriaceae family but not of the Serratia genus. S. fonticola belongs to a group of opportunistic pathogens and can cause illness in people with weak or damaged immune systems. A biochemical characterization of the strain was made by using the identification system API (bioMèrieux®. Molecular characterization was done by PCR amplification of 16S rDNA gene using the thermal cycling sequencing method and by sequencing. By comparing the obtained 1016 nucleotide sequence with the NCBI collection of all deposited sequences for 16S rDNK, and by using the BLAST search service, the highest identity (98% uniformity was obtained with the S. fonticola strain, designated as LMG 7882 (gi|15054669|gb|AF286869.1. The identity of 16S rDNA between the referent strain and ST2 is not absolute, indicating an autochthonous origin of strain ST2.

  6. Gene expression changes in patients with fulminant type 1 diabetes

    Institute of Scientific and Technical Information of China (English)

    WANG Zhen; ZHENG Chao; TAN Yu-yu; LI Yi-jun; YANG Lin; HUANG Gan; LIN Jian; ZHOU Zhi-guang

    2011-01-01

    Background Fulminant type 1 diabetes (F1D) is a complex disease.Microarray analysis was used to identify gene expression changes and obtain understanding of the underlying mechanisms.Methods Microarray analysis was performed on peripheral blood mononuclear cells from six F1D patients and six matched healthy subjects.Real-time polymerase chain reaction was used to verify the differentially expressed genes.NK cell activity was detected by methyl thiazoleterazolium assay.Results Microarray analysis identified 759 genes differing in expression between F1D patients and controls at a false discovery rate of 0.05.Expression of TLR9,ELF4 and IL1RAP were verified and consistent with changes in microarray results.NK cell activity was decreased in F1D.With use of a knowledge base,differentially expressed genes could be placed within different pathways with predicted functions including interleukin-1,and tumor necrosis factor-α signaling.Conclusions These results identify several genes indicating possible mechanisms in F1D.NK cell dysfunction resulting from changes in expression of TLR9,ELF4 and IL1RAP,and pathways of interleukin-1 and tumor necrosis factor-α signaling might be involved in F1D through inducing β-cell dysfunction.

  7. New Variants of Tomato Thymidine Kinase 1 Selected for Increased Sensitivity of E. coli KY895 towards Azidothymidine

    Energy Technology Data Exchange (ETDEWEB)

    Slot Christiansen, Louise [Department of Biology, Lund University, Lund 22362 (Sweden); Lund Protein Production Platform, Lund University, Lund 22362 (Sweden); Egeblad, Louise [Lund Protein Production Platform, Lund University, Lund 22362 (Sweden); Munch-Petersen, Birgitte [Department of Science, Systems and Models, Roskilde University, Roskilde 4000 (Denmark); Piškur, Jure [Department of Biology, Lund University, Lund 22362 (Sweden); Knecht, Wolfgang, E-mail: Louise.Slot_Christiansen@biol.lu.se [Department of Biology, Lund University, Lund 22362 (Sweden); Lund Protein Production Platform, Lund University, Lund 22362 (Sweden)

    2015-06-08

    Nucleoside analogues (NA) are prodrugs that are phosphorylated by deoxyribonucleoside kinases (dNKs) as the first step towards a compound toxic to the cell. During the last 20 years, research around dNKs has gone into new organisms other than mammals and viruses. Newly discovered dNKs have been tested as enzymes for suicide gene therapy. The tomato thymidine kinase 1 (ToTK1) is a dNK that has been selected for its in vitro kinetic properties and then successfully been tested in vivo for the treatment of malignant glioma. We present the selection of two improved variants of ToTK1 generated by random protein engineering for suicide gene therapy with the NA azidothymidine (AZT). We describe their selection, recombinant production and a subsequent kinetic and biochemical characterization. Their improved performance in killing of E. coli KY895 is accompanied by an increase in specificity for the NA AZT over the natural substrate thymidine as well as a decrease in inhibition by dTTP, the end product of the nucleoside salvage pathway for thymidine. The understanding of the enzymatic properties improving the variants efficacy is instrumental to further develop dNKs for use in suicide gene therapy.

  8. Useful and harmful effects of nitric oxide

    Directory of Open Access Journals (Sweden)

    Jović Slavoljub

    2013-01-01

    Full Text Available In living sistems synthesis of nitric oxide occurs during metabolism from Larginin, nitrite and ascorbate. Being very significant carrier of information within numerous both physiological and pathological proceses in mammals' organisms, nitric oxid could possibly be useful as well as harmful. Nitric oxide synthesis is adjuvant in a healthy organism because it represents the basic molecule for understanding numerous processes in neurology, psychology, immunology and varios related fields. In other words, nitric oxide participate in number of physiological processes, such as: transmission of nerve signals (neurotransmitter role, regulation of smooth muscle tissue relaxation (eg. vasodilatation, peristaltic movements, immunomodulation, mastocyte activation, development of inflammatory response, apoptosis regulation, angiogenesis and glucose metabolism, normal heart functioning and antioxidation role. Besides being useful, nitric oxide can be harmful as well, because it has one unpaired electron, so consequently it is susceptible to oxidation becoming a stable free radical. Being such, it reacts quickly with superoxide-anion radical, givind at first an extremely reactive peroxinitrite anion, and subsequently peroxidnitrite acid. This acid is very dangerous causing thiol groups oxidation, tyrosine and phenylalanine nitrosylation, lipid oxidation, DNK chain splitting, nitrification and nucleic bases deamination. These damages of macromolecules can cause a series of undesirable changes which subsequently distub functions of molecules, and thus of cells, tissues and even organs. [Projekat Ministarstva nauke Republike Srbije, br. 173034 i br. 31085

  9. EFFECT OF CATHA EDULIS ON INSULIN, RESISTIN AND CORTISOL LEVELS IN TYPE-2 DIABETICS AND NON-DIABETICS

    Directory of Open Access Journals (Sweden)

    Mohamed Ibrahem Kotb El-Sayed

    2012-01-01

    Full Text Available In this study, the biochemical effects of Catha edulis leaves chewing (as psycho stimulant and aphrodisiac on the serum concentration of resistin, insulin, cortisol, zinc, calcium, copper and blood glucose in both healthy individuals and type 2 diabetic patients were examined. 80 male subjects aged 35-55 years were recruited in this study, 40 of them were previously diagnosed as type 2 diabetics and the other 40 were healthy non-diabetics. The above two groups were subdivided into two subgroups (n = 20 in accordance on whether they were regular and chronic khat chewers or none into NNK; healthy non-khat chewers, NK; healthy khat chewers, DNK; type 2 diabetic non-khat chewers and DK; type 2 diabetic khat chewers. Khat chewing resulted in elevated resistin, cortisol, FBG, PBG levels and HOMA-IR in either diabetics or healthy khat chewers than those of non-khat chewers and generally in diabetics than healthy. In addition, khat chewing resulted in a significant increase in calcium and copper serum levels. In contrast, serum zinc and insulin levels in diabetic chewers were significantly lower than those of diabetic’s non-chewers. Catha edulis Forsk chewing adds additional toxic effects to type 2 diabetics by increasing cortisol and resistin levels while decreasing insulin secretion and sensitivity.

  10. BENIGN EPITHELIAL NEOPLASIA ASSOCIATED WITH BETA-HUMAN PAPILLOMA VIRUS

    Directory of Open Access Journals (Sweden)

    V. A. Molochkov

    2014-01-01

    Full Text Available Aim: To study an association between acrochordon and human papilloma virus (HPV using quantitative analysis of viral desoxyribonucleic acid (DNA; to detect different phenotypes of beta-HPV. Materials and methods: We examined 52  patients (22 immuno-suppressed patients and 30 immunocompetent subjects in the Dermatovenereology and Dermato-Oncology Department and Chronic Dialysis and Kidney Transplantation Department of the Moscow Regional Research and Clinical Institute (MONIKI. Control group included 49 healthy donors. Burr biopsy samples (micro-samples of acrochordon and intact skin (apper arm were collected in sterile conditions. After sample procession and DNA isolation using DNK-sorb-C kit (Central Research Institute for Epidemiology – CRIE, polymerase chain reaction for HPV was performed with real-time fluorescent hybridization detection. For DNA amplification and detection we used RotorGene 3000 analyzer (Corbett Research, Australia. In the beta-HPV assay, recombinant plasmids were used as positive controls and control human beta-globin gene fragments (CRIE. 4 oligo-nucleotide systems (group-specific primers and probes were used for the detection of beta-HPV DNA. Results: Preliminary data indicated that acrochordons of open and covered skin regions were common in renal transplant recipients. Beta-HPV DNA was more frequent in acrochordons and intact skin (64% and 54% of renal transplant recipients compared to healthy donors (47%. 57% of renal transplant recipients demonstrated mixed infection in acrochordons. Conclusion: HPV DNA was frequently detected in acrochordons and intact skin of renal transplant recipients. In immunocompetent patients prevalence of HPV DNA in acrochordons was significantly higher compared to intact skin.

  11. Expression of deoxynucleoside kinases and 5'-nucleotidases in mouse tissues: implications for mitochondrial toxicity.

    Science.gov (United States)

    Rylova, Svetlana N; Mirzaee, Saeedeh; Albertioni, Freidoun; Eriksson, Staffan

    2007-06-30

    Anti-HIV nucleoside therapy can result in mitochondrial toxicity affecting muscles, peripheral nerves, pancreas and adipose tissue. The cytosolic deoxycytidine kinase (dCK; EC 2.7.1.74) and thymidine kinase (TK1; EC 2.7.1.21), the mitochondrial thymidine kinase (TK2) and deoxyguanosine kinase (dGK; EC 2.7.1.113) as well as 5'-deoxynucleotidases (5'-dNT; EC 3.1.3.5) are enzymes that control rate-limiting steps in formation of intracellular and intra-mitochondrial nucleotides. The mRNA levels and activities of these enzymes were determined in mouse tissues, using real-time PCR and selective enzyme assays. The expression of mRNA for all these enzymes and the mitochondrial deoxynucleotide carrier was detected in all tissues with a 5-10-fold variation. TK1 activities were only clearly detected in spleen and testis, while TK2, dGK and dCK activities were found in all tissues. dGK activities were higher than any other dNK in all tissues, except spleen and testis. In skeletal muscle dGK activity was 5-fold lower, TK2 and dCK levels were 10-fold lower as compared with other tissues. The variation in 5'-dNT activities was about eight-fold with the highest levels in brain and lowest in brown fat. Thus, the salvage of deoxynucleosides in muscles is 5-10-fold lower as compared to other non-proliferating tissues and 100-fold lower compared to spleen. These results may help to explain tissue specific toxicity observed with nucleoside analogs used in HIV treatment as well as symptoms in inherited mitochondrial TK2 deficiencies.

  12. Biological phosphorylation of an Unnatural Base Pair (UBP) using a Drosophila melanogaster deoxynucleoside kinase (DmdNK) mutant

    Science.gov (United States)

    Daugherty, Ashley B.; Yang, Zunyi; Shaw, Ryan; Dong, Mengxing; Lutz, Stefan; Benner, Steven A.

    2017-01-01

    One research goal for unnatural base pair (UBP) is to replicate, transcribe and translate them in vivo. Accordingly, the corresponding unnatural nucleoside triphosphates must be available at sufficient concentrations within the cell. To achieve this goal, the unnatural nucleoside analogues must be phosphorylated to the corresponding nucleoside triphosphates by a cascade of three kinases. The first step is the monophosphorylation of unnatural deoxynucleoside catalyzed by deoxynucleoside kinases (dNK), which is generally considered the rate limiting step because of the high specificity of dNKs. Here, we applied a Drosophila melanogaster deoxyribonucleoside kinase (DmdNK) to the phosphorylation of an UBP (a pyrimidine analogue (6-amino-5-nitro-3-(1’-b-d-2’-deoxyribofuranosyl)-2(1H)-pyridone, Z) and its complementary purine analogue (2-amino-8-(1’-b-d-2’-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one, P). The results showed that DmdNK could efficiently phosphorylate only the dP nucleoside. To improve the catalytic efficiency, a DmdNK-Q81E mutant was created based on rational design and structural analyses. This mutant could efficiently phosphorylate both dZ and dP nucleoside. Structural modeling indicated that the increased efficiency of dZ phosphorylation by the DmdNK-Q81E mutant might be related to the three additional hydrogen bonds formed between E81 and the dZ base. Overall, this study provides a groundwork for the biological phosphorylation and synthesis of unnatural base pair in vivo. PMID:28323896

  13. 淋病奈瑟菌pI优势基因型及其G120/A121突变与耐药性关系%Correlation between predominant pI genotypes,G120/A121 mutations and drug resistance

    Institute of Scientific and Technical Information of China (English)

    高剑云; 陈建忠; 赵金方; 周坚红

    2010-01-01

    Objective To analyze the predominant genotypes of outer membrane porin I (PI)and to determine the correlation between G120 as well as A121 mutations in PI proteins and drug resistance in Neisseria gonorrhoeae isolates in the local area.Methods A double PCR to simultaneously detect both pIA and pIB genes,was established in this study.The target amplification products were T-A cloned and then sequenced to determine the mutations at G120,A121 and the specificity of double PCR.By using acidity slip method and double agar dilution method,the β-lactamase production and resistance to six antibiotics of pIA~+ and pIB~+ gonoeoeeal isolates were detected.Results Double PCR could be used to accurately genotyping pI genes in all the tested gonococcal isolates with the sensitivity of 1 ng DNA template.In the 116 N.gonorrhoeae isolates,30.2%(35/116) were pIA~+ strains and 69.8%(81/136) were pIB~+ strains.All the pIA~+ strains presented G120D/A121G double mutations (88.6%) or A121G single mutation (11.4%).98.8% of the pIB +strains presented G120K/A121D (65.0%),G120K/A121G or G120N/A121D ( 13.8% ) double mutations,and G120D/N/K single mutation (21.3% ).34.5% (40/116) of the isolates produced β-lactamase,and the enzyme-produced rate (20%) in pIA~+ strains was significantly lower than that in pIB~+ strains (40.7%) with P<0.05.No spectinomycin-resistant swains were identified but three ceftriaxoneresistant strains were presented.However,the resistance ratios to penicilin,terramycin,ciprofloxacin and azithromycin of all the isolates were as high as 75.0%-90.5%.100% and 71.4% of the pIA~+strains without β-lactamase production and with G120 and/or A121 mutations were sensitive topenicillin and terramycin,respectively.On the contrast,100% of the pIB~+ strains without β-1actamase production and with G120 and/or A121 mutations were resistant to both the two antibiotics.Conclusion The established double PCR method could be used for fast and accurate genotyping of N.gonorrhoeae p