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Sample records for delphinium occidentale chemotypes

  1. Modern garden delphiniums

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    Bassett, Shirley E.

    1990-12-01

    Full Text Available The characteristic features or modern English garden hybrid delphiniums are described. The development by Reinelt of the «Pacific» seed strain in America, and the successful introduction or red colours by Legro in Holland are discussed. The evolution of the tetraploid garden hybrid is considered in the light of species available to early breeders. The role of the Delphinium Society in the promotion of the flower and the encouragement or breeding programs is reviewed.

    [ca] Es descriuen les característiques dels delphiniums híbrids de jardí anglesos moderns. Es discuteix el desenvolupament de la raça de granes «Pacific» a Amèrica per Reinelt i l'èxit de la introducció de colors vermells a Holanda per Legro. L'evolució dels híbrids de jardineria tetraploides és considerada a la llum de les espècies que eren disponibles per als primers milloradors. Es revisa el paper de la Delphinium Society en la promoció de la flor i en la promoció dels programes de millora.

  2. Alkaloids from Delphinium pentagynum.

    Science.gov (United States)

    Díaz, Jesús G; Ruiz, Juan García; Herz, Werner

    2004-07-01

    Aerial parts of a collection of Delphinium pentagynum Lam. from Niebla, Southern Spain, furnished one diterpene alkaloid, 2-dehydrodeacetylheterophylloidine, two norditerpene alkaloids, 14-demethyl-14-isobutyrylanhweidelphinine and 14-demethyl-14-acetylanhweidelphinine, the known alkaloids 14-deacetylnudicauline, methyllycaconitine, 14-deacetyl-14-isobutyrylnudicauline, 14-acetylbrowniine, browniine, delcosine, lycoctonine, 18-methoxygadesine, neoline, karakoline and the aporphine alkaloid magnoflorine. Structures of the alkaloids were established by MS, 1D and 2-D NMR techniques.

  3. Biological and pharmacological effects of Delphinium elbursense

    African Journals Online (AJOL)

    GREGORY

    2010-08-23

    Aug 23, 2010 ... showed weak antioxidant activity in some models. IC50 for 1,1-diphenyl-2-picryl ... assessed in a variety of animal models (Zhang, 2004). Delphinium ..... of individual compounds, their in vivo antioxidant activities and different ...

  4. Two new diterpene alkaloids from Delphinium chrysotrichum

    Institute of Scientific and Technical Information of China (English)

    Yang Qing He; Xiao Mei Wei; Yi Li Han; Li Ming Gao

    2007-01-01

    Chemical investigation on the ethanol extract from the whole plants of Delphinium chrysotrichum resulted in the isolation of two new diterpene alkaloids named delphatisine A (1) and delphatisine B (2), respectively. The structures of the new compounds were deduced on the basis of their spectral data (IR, HREIMS, EIMS, 1D, 2D-NMR). This is the first report on the isolation of diterpenoid alkaloids from the D. Chrysotrichum.

  5. Two New C19-Diterpenoid Alkaloids from Delphinium davidii Franch.

    Institute of Scientific and Technical Information of China (English)

    Xiao Xia LIANG; Dong Lin CHEN; Feng Peng WANG

    2006-01-01

    Two new C19-diterpenoid alkaloids, davidisines A (1) and B (2) along with thirteen known alkaloids were isolated from the whole herb of Delphinium davidii Franch. Their structures were established by spectral methods, especially 2D NMR techniques.

  6. Delphinium L. Subgen. Delphinium: origin and evolutionary trends

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    Blanché, Cèsar

    1990-12-01

    Full Text Available The advanced features of Delphinium lo subgen. Delphinium(annual taxa are compared with those of subgen. Delphinastrum (DC. Wang and subgen. Oligophyllon Dimitrova (perennial taxa. F1ower morphology shows a functional interchange between lateral petals and upper petals. and inflorescence structure favours geitonogamy-autogamy rates. Karyotype evolution is based on a decrease in the total length of chromosomes and on an increase in the degree of asymmetry: the chromosome number remains constant (2n = 16 in the subgen. Delphinium.The dispersal efficiency of the annual species is higher than that of perennials due to seed set increase and improved floatability. both in water and in air. Other advanced adaptive features are the acquisition or new chemical weapons and the emergence of a new embryonogenic type. The ecological niches or subgen. Delphinium correspond to open and disturbed habitats in contrast to the stable and relatively closed ones or subgenera Delphinastum and Oligophyllon. A final hypothesis is made concerning the evolutionary trends observed in annuals vs. perennials in connexion with biogeographical considerations, and a final taxonomic summary is given.

    [ca] Les característiques avançades de Delphinium L. subgèn. Delphinium (tàxons anuals són comparades amb les del subgèn. Delphinastrum (DC. Wang i del subgèn. Oligophyllon Dimitrova (tàxons perennes. La morfologia norai mostra un intercanvi de funcions entre els pètals laterals i els pètals superiors i l'estructura de la inflorescència de molts tàxons anuals afavoreix un augment de les taxes de geitonogàmia-autogàmia. L'evolució dels cariotips és basada en una disminució de la longitud total dels cromosomes i en un increment del grau d'asimetria; el nombre cromosòmic roman constant per a totes les espècies anuals (2n = 16. l'eficàcia de la dispersió de les esp

  7. Cytotaxonomy of North African species of Delphinium L. sect. Delphinium (Ranunculaceae

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    Blanché, César

    1990-05-01

    Full Text Available Both chromosome numbers and karyotype structure from 15 wild North-African populations of Delphinium L. sect. Delphinium were studied. Delphinium balansae Boiss. & Reuter, with a more symmetrical karyotype, appears as a possible perennial ancestor of' the remaining annuals with highly asymmetrical and shorter karyotype. A taxonomical reorganization of this section is proposed: two new series (ser. Macropetala ser. nova and ser. Balansae ser. nova. and two new nomenclatural combinations (D. nanum subsp. alboliliaceum and D. nanum subsp. elongatum are proposed.

    S'han estudiat els nombres cromosòmics i l'estructura deis cariotipus de 15 poblacions nordafricanes de Delphinium L. sect. Delphinium. Delphinium balansae Boiss. & Reuter, amb cariotipus mes simètric, apareix com a possible ancestre perenne de la resta d’espècies anuals amb cariotipus més asimètric i més curt. Es proposa una reorganització taxonòmica de la secció, d'on es descriu en dues series noves (ser. Macropetala ser. nova i ser. Balansae ser. nova. i es proposen dues combinacions noves (D. nanum subsp. alboliliaceum and D. nanum subsp. elongatum.

  8. New Diterpenoid Alkaloids from the Roots of Delphinium tiantaishanense

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    Feng-Peng Wang

    2007-03-01

    Full Text Available Four new diterpenoid alkaloids: tiantaishansine (1, tiantaishannine (2, tiantaishanmine (3, and tiantaishandine (4 have been isolated from the roots of Delphinium tiantaishan. Their structures were elucidated by chemical evidence andspectral analyses, including ESI-MS, HR-EI-MS, 1D- and 2D-NMR.

  9. The clearance of delphinium alkaloids from the serum of cattle

    Science.gov (United States)

    Larkspurs (Delphinium spp.) (figure 1) commonly poison cattle in many western rangelands of North America. Yearly herd mortality can be as high as 10% with annual economic losses of several millions of dollars in animal deaths, increased management, treatment costs, and the loss of the use of otherw...

  10. Taxonomic and ecological review of California Delphinium

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    Warnock, Michael J.

    1990-12-01

    Full Text Available Included within the boundaries of California are all or parts of the ranges of many of the Delphinium native to North America.Currently, 28 species of Delphinium are recognized as occurring in California. In addition to nominal subspecies in these species. another 17 subspecies are recognized. A key to, and descriptions of, the taxa of Delphinium found in California are presented. 1ncludedwith descriptions are comments on distribution, habitat preferences, propensity for hybridization and abundance of each taxon. Certain hybrids which may be found (often as a result of human disturbance are separated in the key.

    [ca] A l'interior dels límits de Califòrnia es troben la totalitat o parts de les àrees de bona part dels Delphinium nadius de Nordamèrica. Es reconeixen 28 espècies de Delphinium a Califòrnia; ultra llurs respectives subspècies nominals, es reconeixen 17 altres subspècies. Es presenten un a clau i les descripcions corresponents als tàxons presents a Califòrnia. Juntament amb les descripcions, s'hi inclouen comentaris sobre la distribució, preferències d'hàbitat, propensió per a ta hibridació i abundància de cada tàxon. Alguns híbrids que es poden trobar (sovint com a resultat de pertorbacions causades per l'home són separats a les claus.

  11. Morphological characterization of cashew (Anacardium occidentale L.)

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... relation to nut yield that can be used to improve produc- tion of cashew in Malawi. ... cultural Development Division, Press Agriculture Limited and all the small ... DNA fingerprinting of India cashew (Anacardium occidentale L.) ...

  12. Revisiting the Delphinium viscosum Hook. f. & Thoms. (Ranunculaceae complex in the Himalaya

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    Priyanka Agnihotri

    2016-03-01

    Full Text Available The paper highlights the complex nature of Delphinium viscosum Hook. f. & Thoms. in the Himalaya and enumerates two subspecies and two varieties. Critical appraisal of the morphological and distributional data revealed that subsp. viscosum and subsp. gigantobracteum having two varieties var. gigantobracteum and chrysotrichum are distinct. Delphinium viscosum complex is solved out and two subspecies and two varieties with clear cut distinct macro and micro-morphological characters have been identified. The taxonomic status of different taxa of this complex species has been established. Delphinium viscosum, a taxonomic complex species is described and illustrated.

  13. Adverse Effects of Larkspur (Delphinium spp. on Cattle

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    Kevin D. Welch

    2015-07-01

    Full Text Available There are numerous species of larkspur (Delphinium spp. in North America. Larkspurs are a major cause of cattle losses on western ranges in the USA, especially on foothill and mountain rangelands. The toxicity of larkspur species is due to various norditerpenoid alkaloids. In this article, we review the current knowledge regarding larkspur ecology and distribution, analytical technologies to study and quantify the toxins in larkspur, the toxicology of the larkspur plants and their individual toxins, known genetic variations in larkspur susceptibility, and current management recommendations to mitigate losses from larkspur poisoning.

  14. Three new C19-diterpenoid alkaloids from Delphinium laxicymosum var. Pilostachyum

    Institute of Scientific and Technical Information of China (English)

    Ping Tang; Dong Lin Chen; Qiao Hong Chen; Xi Xian Jian; Feng Peng Wang

    2007-01-01

    Three new lycoctonine-type C19-diterpenoid alkaloids, laxicymine 1, laxicymisine 2, and laxicyminine 3 have been isolated from the whole herb of Delphinium laxicymosum var.pilostachyum W.T.Wang.Their structures were established by spectra data.

  15. Leishmanicidal Activity of Nine Novel Flavonoids from Delphinium staphisagria

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    Inmaculada Ramírez-Macías

    2012-01-01

    Full Text Available Objectives. To evaluate the in vitro leishmanicidal activity of nine flavonoid derivatives from Delphinium staphisagria against L. infantum and L. braziliensis. Design and Methods. The in vitro activity of compounds 1–9 was assayed on extracellular promastigote and axenic amastigote forms and on intracellular amastigote forms of the parasites. Infectivity and cytotoxicity tests were carried on J774.2 macrophage cells using Glucantime as the reference drug. The mechanisms of action were analysed performing metabolite excretion and transmission electronic microscope ultrastructural alteration studies. Results. Nine flavonoids showed leishmanicidal activity against promastigote as well as amastigote forms of Leishmania infantum and L. braziliensis. These compounds were nontoxic to mammalian cells and were effective at similar concentrations up to or lower than that of the reference drug (Glucantime. The results showed that 2″-acetylpetiolaroside (compound 8 was clearly the most active. Conclusion. This study has demonstrated that flavonoid derivatives are active against L. infantum and L. braziliensis.

  16. Three new C20-diterpenoid alkaloids from Delphinium anthriscifolium var. savatieri

    Institute of Scientific and Technical Information of China (English)

    Xiao Yu Liu; Qiao Hong Chen; Feng Peng Wang

    2009-01-01

    Three new C20-diterpenoid alkaloids, designated as anthriscifolmines A-C (1-3), together with two known alkaloids denudatine and delgramine, were isolated from the whole herb of Delphinium anthriscifolium var. savatieri. The structures of these new alkaloids were elucidated on the basis of spectral data.

  17. Effects of Larkspur (Delphinium barbeyi) on Heart Rate and Electrically Evoked Electromyographic Response of the External Anal Sphincter in Cattle.

    Science.gov (United States)

    Norditerpenoid alkaloids of larkspur (Delphinium spp.) are competitive antagonists of nicotinic cholinergic receptors and poison cattle with, high mortality. Of the norditerpenoids, the N-(methylsuccinimido) anthranoyllycoctonine type (MSAL-type) alkaloids are most toxic. This study tested the hyp...

  18. Delphinium fissum subsp. sordidum (Ranunculaceae in Portugal: distribution and conservation status

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    Rubén Ramírez-Rodríguez

    2017-05-01

    Full Text Available Delphinium fissum subsp. sordidum has been found in calcareous and schistaceous slopes on the Mines of Santo Adrião, Trás-os-Montes region —close to Bragança, northeastern Portugal—. It is the first record of this taxon in Portugal and the westernmost locality of its general distribution area. Its conservation status, according to the most recent IUCN categories and criteria, should be “Critically Endangered” in Portugal.

  19. Honatisine, a novel diterpenoid alkaloid, and six known alkaloids from Delphinium honanense and their cytotoxic activity.

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    He, Yang Qing; Ma, Zhan Ying; Wei, Xiao Mei; Liu, Dong Jie; Du, Bao Zhong; Yao, Bing Hua; Gao, Li Ming

    2011-11-01

    A novel diterpene alkaloid named honatisine (1) has been isolated from the whole plants of Delphinium honanense, along with six known alkaloids, siwanine E (2), isoatisine (3), atisine (4), delcorinine (5), uraphine (6), and nordhagenine A (7). Their structures were deduced on the basis of their spectral data. All of them were evaluated by a SRB assay for their cytotoxicity, and compound 1 showed a significant cytotoxic activity (IC(50) =3.16 μM) against the MCF-7 cell line.

  20. Chemotyping of yeast mutants using robotics.

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    Rieger, K J; El-Alama, M; Stein, G; Bradshaw, C; Slonimski, P P; Maundrell, K

    1999-07-01

    By now, the EUROFAN programme for the functional analysis of genes from the yeast genome has attained its cruising speed. Indeed, several hundreds of yeast mutants with no phenotype as tested by growth on standard media and no significant sequence similarity to proteins of known function are available through the efforts of various laboratories. Based on the methodology initiated during the pilot project on yeast chromosome III (Yeast 13, 1547-1562, 1997) we adapted it to High Throughput Screening (HTS), using robotics. The first 100 different gene deletions from EUROSCARF, constructed in an FY1679 strain background, were run against a collection of about 300 inhibitors. Many of these inhibitors have not been reported until now to interfere in vivo with growth of Saccharomyces cerevisiae. In the present paper we provide a list of novel growth conditions and a compilation of 49 yeast deletants (from chromosomes II, IV, VII, X, XIV, XV) corresponding to 58% of the analysed genes, with at least one clear and stringent phenotype. The majority of these deletants are sensitive to one or two compounds (monotropic phenotype) while a distinct subclass of deletants displays a hyper-pleiotropic phenotype with sensitivities to a dozen or more compounds. Therefore, chemotyping of unknown genes with a large spectrum of drugs opens new vistas for a more in-depth functional analysis and a more precise definition of molecular targets.

  1. Soft coral Sarcophyton (Cnidaria: Anthozoa: Octocorallia) species diversity and chemotypes.

    Science.gov (United States)

    Aratake, Satoe; Tomura, Tomohiko; Saitoh, Seikoh; Yokokura, Ryouma; Kawanishi, Yuichi; Shinjo, Ryuichi; Reimer, James Davis; Tanaka, Junichi; Maekawa, Hideaki

    2012-01-01

    Research on the soft coral genus Sarcophyton extends over a wide range of fields, including marine natural products and the isolation of a number of cembranoid diterpenes. However, it is still unknown how soft corals produce this diverse array of metabolites, and the relationship between soft coral diversity and cembranoid diterpene production is not clear. In order to understand this relationship, we examined Sarcophyton specimens from Okinawa, Japan, by utilizing three methods: morphological examination of sclerites, chemotype identification, and phylogenetic examination of both Sarcophyton (utilizing mitochondrial protein-coding genes MutS homolog: msh1) and their endosymbiotic Symbiodinium spp. (utilizing nuclear internal transcribed spacer of ribosomal DNA: ITS- rDNA). Chemotypes, molecular phylogenetic clades, and sclerites of Sarcophyton trocheliophorum specimens formed a clear and distinct group, but the relationships between chemotypes, molecular phylogenetic clade types and sclerites of the most common species, Sarcophyton glaucum, was not clear. S. glaucum was divided into four clades. A characteristic chemotype was observed within one phylogenetic clade of S. glaucum. Identities of symbiotic algae Symbiodinium spp. had no apparent relation to chemotypes of Sarcophyton spp. This study demonstrates that the complex results observed for S. glaucum are due to the incomplete and complex taxonomy of this species group. Our novel method of identification should help contribute to classification and taxonomic reassessment of this diverse soft coral genus.

  2. Soft coral Sarcophyton (Cnidaria: Anthozoa: Octocorallia species diversity and chemotypes.

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    Satoe Aratake

    Full Text Available Research on the soft coral genus Sarcophyton extends over a wide range of fields, including marine natural products and the isolation of a number of cembranoid diterpenes. However, it is still unknown how soft corals produce this diverse array of metabolites, and the relationship between soft coral diversity and cembranoid diterpene production is not clear. In order to understand this relationship, we examined Sarcophyton specimens from Okinawa, Japan, by utilizing three methods: morphological examination of sclerites, chemotype identification, and phylogenetic examination of both Sarcophyton (utilizing mitochondrial protein-coding genes MutS homolog: msh1 and their endosymbiotic Symbiodinium spp. (utilizing nuclear internal transcribed spacer of ribosomal DNA: ITS- rDNA. Chemotypes, molecular phylogenetic clades, and sclerites of Sarcophyton trocheliophorum specimens formed a clear and distinct group, but the relationships between chemotypes, molecular phylogenetic clade types and sclerites of the most common species, Sarcophyton glaucum, was not clear. S. glaucum was divided into four clades. A characteristic chemotype was observed within one phylogenetic clade of S. glaucum. Identities of symbiotic algae Symbiodinium spp. had no apparent relation to chemotypes of Sarcophyton spp. This study demonstrates that the complex results observed for S. glaucum are due to the incomplete and complex taxonomy of this species group. Our novel method of identification should help contribute to classification and taxonomic reassessment of this diverse soft coral genus.

  3. Seed morphology of the genus Delphinium L. (Ranunculaceae in Turkey

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    Ilarsan, Hilal

    1997-12-01

    Full Text Available The seed morphology or 28 species or Delphinium collected from Turkey is surveyed by Scanning Electron Microscopy (SEM. Four major seed types are reported; subglobose with continous rings or lamellae. subpyramidal with narrow or wide scales, elongate subpyramidal with scales and sector-sphercidal reticulate, corresponding with the sections recognized in the current taxonomic systems or the genus, thus indicating its good taxonomic resolution. Biogeographical and systematic implications are discussed.

    La morfologia de les granes de 28 espècies de Delphinium recollides a Turquia ha estat estudiada per mitjà de la Microscòpia Electrònica de Rastreig (MER. Quatre tipus principals de granes són reportats: subgloboses amb anells continus de lamelles, subpiramidals amb esquames amples o primes, subpiramidals allargades amb esquames i sector-esferoïdals reticulades, que es corresponen amb les seccions reconegudes en els sistemes taxonòmics habituals del gènere, cosa que indica llur bona resolució taxonòmica. Es discuteixen les implicacions biogeogràfiques i sistemàtiques.

  4. Discrimination of fennel chemotypes applying IR and Raman spectroscopy: discovery of a new γ-asarone chemotype.

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    Gudi, Gennadi; Krähmer, Andrea; Krüger, Hans; Hennig, Lothar; Schulz, Hartwig

    2014-04-23

    Various vibrational spectroscopy methods have been applied to classify different fennel chemotypes according to their individual profile of volatile substances. Intact fennel fruits of different chemotypes could be successfully discriminated by attenuated total reflectance Fourier transform infrared (ATR-FTIR) and near infrared (NIR) spectroscopy. Solvent extracts (CCl4) of the considered fennel fruits showed characteristic fingerprints with marker bands related to the individual volatile components (trans-anethole, fenchone, estragole, piperitenone oxide, γ-asarone, limonene) for ATR-FTIR and FT-Raman spectroscopy. Especially νC═C and νC═O absorption bands contribute to the different spectral profiles. On the basis of hierarchical cluster analysis, the considered fennel accessions were classified according to gas chromatographic (GC) and vibrational spectroscopic data. Furthermore, even a discrimination of "sweet" and "bitter" fennel fruits, both belonging to the trans-anethole chemotype, could be successfully performed. All vibrational spectroscopical techniques used in this study are rapid and easy to apply. Hence, they allow different fennel chemotypes to be reliably distinguished and can also be used for on-site measurement in free nature.

  5. Discrimination of fennel chemotypes applying IR and Raman spectroscopy – discovery of a new -asarone chemotype

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    Krähmer, Andrea

    2016-07-01

    Full Text Available Various vibrational spectroscopy methods have been applied to classify different fennel chemotypes according to their individual profile of volatile substances. Intact fennel fruits of different chemotypes could be successfully discriminated by Attenuated Total Reflectance Fourier transform Infrared (ATR-FTIR and Near Infrared (NIR spectroscopy. Solvent extracts (CCl4 of the considered fennel fruits showed characteristic fingerprints with marker bands related to the individual volatile components (trans-anethole, fenchone, estragole, piperitenone oxide, -asarone, limonene for ATR-FTIR and FT-Raman spectroscopy. Especially C=C and C=O absorption bands contribute to the different spectral profiles. Based on hierarchical cluster analysis, the considered fennel accessions were classified according to gas chromatographic (GC and vibrational spectroscopic data. Furthermore, even a discrimination of “sweet” and “bitter” fennel fruits, both belonging to the trans-anethole chemotype, could be successfully performed. All vibrational spectroscopical techniques used in this study are rapid and easy to apply. Hence, they allow different fennel chemotypes to be reliably distinguished and can also be used for on-site measurement in free nature.

  6. UNA NUOVA SPECIE DI TRIODONTELLA DELLA SARDEGNA OCCIDENTALE (COLEOPTERA, MELOLONTHIDAE

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    Piero Leo

    2007-10-01

    Full Text Available Viene descritta Triodontella mimula n. sp. della Sardegna occidentale (locus typicus.: Oristano, foce fiume Tirso, affine a T. raymondi (Perris, 1869 ma nettamente distinta per la forma dei parameri. Viene definito su base morfologica il gruppo naturale di specie di Triodontella raymondi e si discute l’ecologia e la faunistica di T. mimula n. sp. e T. raymondi, evidenziandone la vicarianza in Sardegna.

  7. Green synthesis of silver nanoparticles using Delphinium denudatum root extract exhibits antibacterial and mosquito larvicidal activities.

    Science.gov (United States)

    Suresh, Gopal; Gunasekar, Poosali Hariharan; Kokila, Dhanasegaran; Prabhu, Durai; Dinesh, Devadoss; Ravichandran, Nagaiya; Ramesh, Balasubramanian; Koodalingam, Arunagirinathan; Vijaiyan Siva, Ganesan

    2014-06-05

    Green synthesis of silver nanoparticles (AgNPs) using aqueous root extract of Delphinium denudatum (Dd) by reduction of Ag(+) ions from silver nitrate solution has been investigated. The synthesized DdAgNPs were characterized by using UV-Vis spectroscopy, X-ray diffraction (XRD), Field emission scanning electron microscope (FESEM) and Fourier transform infrared spectroscopy (FTIR). The prepared DdAgNPs showed maximum absorbance at 416nm and particles were polydispersed in nature, spherical in shape and the size of the particle obtained was⩽85nm. The DdAgNPs exhibited antibacterial activity against Staphylococcus aureus ATCC 6538, Bacillus cereus NCIM 2106, Escherichia coli ATCC 8739 and Pseudomonas aeruginosa ATCC 9027. The DdAgNPs showed potent larvicidal activity against second instar larvae of dengue vector Aedes aegypti with a LC50 value of 9.6ppm.

  8. Green synthesis of silver nanoparticles using Delphinium denudatum root extract exhibits antibacterial and mosquito larvicidal activities

    Science.gov (United States)

    Suresh, Gopal; Gunasekar, Poosali Hariharan; Kokila, Dhanasegaran; Prabhu, Durai; Dinesh, Devadoss; Ravichandran, Nagaiya; Ramesh, Balasubramanian; Koodalingam, Arunagirinathan; Vijaiyan Siva, Ganesan

    2014-06-01

    Green synthesis of silver nanoparticles (AgNPs) using aqueous root extract of Delphinium denudatum (Dd) by reduction of Ag+ ions from silver nitrate solution has been investigated. The synthesized DdAgNPs were characterized by using UV-Vis spectroscopy, X-ray diffraction (XRD), Field emission scanning electron microscope (FESEM) and Fourier transform infrared spectroscopy (FTIR). The prepared DdAgNPs showed maximum absorbance at 416 nm and particles were polydispersed in nature, spherical in shape and the size of the particle obtained was ⩽85 nm. The DdAgNPs exhibited antibacterial activity against Staphylococcus aureus ATCC 6538, Bacillus cereus NCIM 2106, Escherichia coli ATCC 8739 and Pseudomonas aeruginosa ATCC 9027. The DdAgNPs showed potent larvicidal activity against second instar larvae of dengue vector Aedes aegypti with a LC50 value of 9.6 ppm.

  9. A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose-dependent glucosyltransferase in the petals of carnation and delphinium.

    Science.gov (United States)

    Matsuba, Yuki; Sasaki, Nobuhiro; Tera, Masayuki; Okamura, Masachika; Abe, Yutaka; Okamoto, Emi; Nakamura, Haruka; Funabashi, Hisakage; Takatsu, Makoto; Saito, Mikako; Matsuoka, Hideaki; Nagasawa, Kazuo; Ozeki, Yoshihiro

    2010-10-01

    Glucosylation of anthocyanin in carnations (Dianthus caryophyllus) and delphiniums (Delphinium grandiflorum) involves novel sugar donors, aromatic acyl-glucoses, in a reaction catalyzed by the enzymes acyl-glucose-dependent anthocyanin 5(7)-O-glucosyltransferase (AA5GT and AA7GT). The AA5GT enzyme was purified from carnation petals, and cDNAs encoding carnation Dc AA5GT and the delphinium homolog Dg AA7GT were isolated. Recombinant Dc AA5GT and Dg AA7GT proteins showed AA5GT and AA7GT activities in vitro. Although expression of Dc AA5GT in developing carnation petals was highest at early stages, AA5GT activity and anthocyanin accumulation continued to increase during later stages. Neither Dc AA5GT expression nor AA5GT activity was observed in the petals of mutant carnations; these petals accumulated anthocyanin lacking the glucosyl moiety at the 5 position. Transient expression of Dc AA5GT in petal cells of mutant carnations is expected to result in the transfer of a glucose moiety to the 5 position of anthocyanin. The amino acid sequences of Dc AA5GT and Dg AA7GT showed high similarity to glycoside hydrolase family 1 proteins, which typically act as β-glycosidases. A phylogenetic analysis of the amino acid sequences suggested that other plant species are likely to have similar acyl-glucose-dependent glucosyltransferases.

  10. Qualitative and quantitative variation in monoterpene co-occurrence and composition in the essential oil of Thymus vulgaris chemotypes.

    Science.gov (United States)

    Thompson, John D; Chalchat, Jean-Claude; Michet, André; Linhart, Yan B; Ehlers, Bodil

    2003-04-01

    Thymus vulgaris has a chemical polymorphism with six different chemotypes that show marked spatial segregation in nature. Although some populations have a single chemotype in majority, many have two or three chemotypes. In this study we analyze the quantitative variation among T. vulgaris populations in the percentage of oil composed of the dominant monoterpene(s) for each chemotype. In general, phenolic chemotypes (thymol and carvacrol), which occur at the end of the biosynthetic chain, have a significantly lower proportion of their oil composed of their dominant monoterpene than nonphenolic chemotypes (geraniol, alpha-terpineol, and linalool). This is due to the presence of high amounts of precursors (gamma-terpinene and paracymene) in the oil of phenolic chemotypes. The essential oil of the nonphenolic thuyanol chemotype has four characteristic monoterpenes that together make up a lower proportion of the oil than the single dominant monoterpene of the other nonphenolic chemotypes. For all chemotypes, the percentage composition of the dominant monoterpene decreased significantly at sites where the chemotype is not the majority type. This decrease is correlated with a significant increase in either the proportion of the two precursors for the thymol chemotype or the monoterpenes characteristic of the other chemotypes at the site. The latter result suggests that a plant with dominant genes is responsible for the production of different monoterpenes can produce several molecules.

  11. Genotoxicity and cytotoxicity of oxindole alkaloids from Uncaria tomentosa (cat's claw): Chemotype relevance.

    Science.gov (United States)

    Kaiser, Samuel; Carvalho, Ânderson Ramos; Pittol, Vanessa; Dietrich, Fabrícia; Manica, Fabiana; Machado, Michel Mansur; de Oliveira, Luis Flávio Souza; Oliveira Battastini, Ana Maria; Ortega, George González

    2016-08-02

    Uncaria tomentosa (Willdenow ex Roemer & Schultes) DC. (Rubiaceae) or cat's claw is a climber vine from the South American rainforest used in folk medicine for cancer treatment. Its antitumor activity has been mostly ascribed to pentacyclic oxindole alkaloids (POA) from stem bark and leaves while the activity of tetracyclic oxindole alkaloids (TOA) remains unknown. In recent times, the occurrence of three chemotypes based on its oxindole alkaloid profile was noticed in U. tomentosa, namely, chemotype I (POA cis D/E ring junction); chemotype II (POA trans D/E ring junction) or chemotype III (TOA). Consequently, the relationship between the chemotype and cytotoxic and genotoxic activities deserves attention. To evaluate the influence of cat's claw chemotypes on genotoxicity and cytotoxicity against non malignant and malignant human cell line models. Four authentic stem bark cat's claw samples (SI-SIV) and two leaf samples (LII and LIII) were analyzed by HPLC-PDA, properly extracted and fractioned by ion-exchange to obtain oxindole alkaloid purified fractions (OAPFs). The freeze-dried fractions were assayed for genotoxicity and cytotoxicity against human leukocytes (non malignant cell line) by the micronuclei frequency method and the alkaline comet DNA assay, and the trypan blue method, respectively. Moreover, the cytotoxicity of each OAPF was evaluated against a human bladder cancer cell line (T24) and human glioblastoma cell line (U-251-MG) by MTT method (malignant cell lines). Additionally, the isomerization of oxindole alkaloids throughout the course of cell incubation was monitored by HPLC-PDA. Based on HPLC-PDA analyses, sample SI was characterized as chemotype I, while samples SII and LII were characterized as chemotype II, and samples SIII, SIV and LIII as chemotype III. The chemotypes showed comparable cytotoxic activity toward malignant cell lines (T24 and U-251-MG) unlike human leukocytes (non malignant cell line), where this activity was clearly distinct

  12. Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum

    NARCIS (Netherlands)

    Li, H.P.; Wu, A.B.; Zhao, C.S.; Scholten, O.E.; Löffler, H.J.M.; Liao, Y.C.

    2005-01-01

    Based on the intergenic sequences of Tri5¿Tri6 genes involved in the mycotoxin pathways of Fusarium species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)-chemotypes and a 360 bp sequence of nivalenol (NIV)- chemotypes of Fusarium graminearum. Mycotoxin

  13. Activity cliffs and activity cliff generators based on chemotype-related activity landscapes.

    Science.gov (United States)

    Pérez-Villanueva, Jaime; Méndez-Lucio, Oscar; Soria-Arteche, Olivia; Medina-Franco, José L

    2015-11-01

    Activity cliffs have large impact in drug discovery; therefore, their detection and quantification are of major importance. This work introduces the metric activity cliff enrichment factor and expands the previously reported activity cliff generator concept by adding chemotype information to representations of the activity landscape. To exemplify these concepts, three molecular databases with multiple biological activities were characterized. Compounds in each database were grouped into chemotype classes. Then, pairwise comparisons of structure similarities and activity differences were calculated for each compound and used to construct chemotype-based structure-activity similarity (SAS) maps. Different landscape distributions among four major regions of the SAS maps were observed for different subsets of molecules grouped in chemotypes. Based on this observation, the activity cliff enrichment factor was calculated to numerically detect chemotypes enriched in activity cliffs. Several chemotype classes were detected having major proportion of activity cliffs than the entire database. In addition, some chemotype classes comprising compounds with smooth structure activity relationships (SAR) were detected. Finally, the activity cliff generator concept was applied to compounds grouped in chemotypes to extract valuable SAR information.

  14. Trichothecene chemotype composition of Fusarium graminearum and related species in Finland and Russia

    Science.gov (United States)

    Fusarium graminearum and type B trichothecene producers can be divided into three chemotypes. Analysis of 290 single-spore isolates of F. graminearum and related Fusarium species revealed that all F. graminearum isolates from Finland (15) and western Russian (26) possessed the 3ADON chemotype, whil...

  15. Greenhouse studies reveal increased aggressiveness of emergent Canadian Fusarium graminearum chemotypes in wheat

    Science.gov (United States)

    The role of Fusarium graminearum trichothecene-chemotypes in disease outcomes was evaluated in a series of wheat lines with different levels of resistance to Fusarium Head Blight (FHB). Four inocula, each consisting of a composite of four strains with either 15-acetyldeoxynivalenol (ADON) chemotypes...

  16. Mutational Changes In Delphinium malabaricum (Huth. Munz.: A Potential Ornamental Plant

    Directory of Open Access Journals (Sweden)

    Kolar Firdose R.

    2015-12-01

    Full Text Available Mutation breeding is an established method used for crop improvement and has played a major role in the development of many new flower color/shape mutant cultivars in ornamentals. The present study is aimed at inducing mutations in Delphinium malabaricum using chemical mutagens ethyl methane sulfonate (EMS, sodium azide (SA and physical mutagen (gamma rays. It was observed that D. malabaricum manifested specific reactions to the treatments with EMS, SA and gamma rays. Identification and selection of mutations were carried out in the second generation (M2. A variety of chlorophyll deficient mutants and high percentage of the flower color and morphological mutants were recorded. The maximum frequency of chlorophyll and flower color and morphological mutations were recorded in EMS treated plants when compared to the other two mutagens. The frequency values for the individual mutant types were varied and randomly distributed at different mutagenic treatments. The highest percentage of color mutants arose after treatments with 0.25% of EMS and the lowest at 20 kR of gamma rays. The mutants were quite distinct, as compared to the control and often had more attractive ornamental features compared to the starting material. The major commercial benefit of the application of this technology has so far been obtaining of novel flower mutants that can be used as an initial material for further breeding of new cultivars.

  17. Occurrence of Stachybotrys chartarum chemotype S in dried culinary herbs.

    Science.gov (United States)

    Biermaier, Barbara; Gottschalk, Christoph; Schwaiger, Karin; Gareis, Manfred

    2015-02-01

    Stachybotrys (S.) chartarum is an omnipresent cellulolytic mould which produces secondary metabolites, such as the highly toxic macrocyclic trichothecenes. While it is known to occur in animal feed like hay and straw as well as in water-damaged indoor environments, there is little knowledge about the occurrence of S. chartarum and its secondary metabolites in food. The objective of the present study was to examine selected dried culinary herbs for the presence of S. chartarum chemotype S, to assess the potential risk of a contamination of foods with macrocyclic trichothecenes. In total, 50 Stachybotrys isolates from different types of culinary herbs (n=100) such as marjoram (Origanum majorana Linné (L.)), oregano (Origanum vulgare L.), thyme (Thymus vulgaris L.), and savory (Satureja hortensis L.) were examined by MTT-cell culture test (effect-based bioassay), ELISA, and by liquid chromatography tandem mass spectrometry (LC-MS/MS). Selected toxic and non-toxic isolates (n=15) were genetically characterized by PCR and sequencing. Five isolates (10%) were highly toxic in the MTT-cell culture test, and the production of macrocyclic trichothecenes was proven by ELISA and LC-MS/MS. These five isolates were genetically confirmed as S. chartarum chemotype S. To the best of our knowledge, this is the first report about a contamination of dried culinary herbs with toxigenic S. chartarum.

  18. Chemotyping the distribution of vitamin D metabolites in human serum

    Science.gov (United States)

    Müller, Miriam J.; Stokes, Caroline S.; Lammert, Frank; Volmer, Dietrich A.

    2016-02-01

    Most studies examining the relationships between vitamin D and disease or health focus on the main 25-hydroxyvitamin D3 (25(OH)D3) metabolite, thus potentially overlooking contributions and dynamic effects of other vitamin D metabolites, the crucial roles of several of which have been previously demonstrated. The ideal assay would determine all relevant high and low-abundant vitamin D species simultaneously. We describe a sensitive quantitative assay for determining the chemotypes of vitamin D metabolites from serum after derivatisation and ultra-high performance liquid chromatography-electrospray ionisation-tandem mass spectrometry (UHPLC-ESI-MS/MS). We performed a validation according to the ‘FDA Guidance for Industry Bioanalytical Method Validation’. The proof-of-concept of the method was then demonstrated by following the metabolite concentrations in patients with chronic liver diseases (CLD) during the course of a vitamin D supplementation study. The new quantitative profiling assay provided highly sensitive, precise and accurate chemotypes of the vitamin D metabolic process rather than the usually determined 25(OH)D3 concentrations.

  19. Religione e costituzionalismo occidentale. Osmosi e reciproche influenze

    Directory of Open Access Journals (Sweden)

    Francesco Alicino

    2012-10-01

    Full Text Available Contributo sottoposto a valutazione, destinato alla pubblicazione nell’opera a cura di C. Decaro Bonella, Radici religiose e itinerari costituzionali, vol. I, Islam e Occidente, edito dalla casa editrice Carocci, Roma, 2012.SOMMARIO: 1. Introduzione. – 2. I presupposti teologici. – 3. Tendenza teocratica, ma visione profana dei fenomeni normativi. – 4. La doppia faccia della secolarizzazione. – 5. Il costituzionalismo (medievale cristiano. – 6. Le origini del costituzionalismo occidentale. Fra apolitismo radicale … – 7. (segue … e messianismo temporale e rivoluzione scientifica. – 8. Westfalia (1648: la territorializzazione del diritto religioso. – 9. La nuova religione della perfetta ragione. – 10. Dalla fine della sovranità westfaliana, al secondo dopoguerra … – 11. … (segue e alla questione islamica di questo secolo.

  20. Cytogeography of essential oil chemotypes of Eremophila longifolia F. Muell (Scrophulariaceae).

    Science.gov (United States)

    Sadgrove, Nicholas John; Jones, Graham Lloyd

    2014-09-01

    Previous studies have demonstrated that the widely distributed desert plant Eremophila longifolia has at least six geographically defined essential oil chemotypes. The focus of the present study is to extend and enhance information concerning known chemotypes and to investigate the involvement of cell nuclei ploidy in this variation. Forty field collected specimens of E. longifolia were taken from most of the mainland states of Australia then subjected to hydrodistillation to produce essential oils, which were then chemically characterised. Ploidy was determined using relative fluorescence of cell nuclei stained with propidium iodide, measured in a flow cytometer. Using principal component analysis (PCA), at least three essential oil chemotypes, in addition to the six already described, were identified in the present study. Previously described high yielding essential oil chemotypes were also characterised in terms of diploidy. For the first time diploid populations were identified in New South Wales, correlating with high yielding isomenthone/menthone and karahanaenone chemotypes. Furthermore, the separate diploid population previously described from Western Australia was demonstrated to be the safrole/methyl eugenol type, which is restricted to a small geographic range in far north-west Western Australia (Murchison District). All other chemotypes were shown to be tetraploid, including apparently randomly emerging individuals, representative of chemotypes producing low yields of isomenthone/menthone and karahanaenone similar in composition to the high yielding diploid types.

  1. Effect of Origanum chemotypes on broiler intestinal bacteria.

    Science.gov (United States)

    Betancourt, Liliana; Rodriguez, Fernando; Phandanouvong, Vienvilay; Ariza-Nieto, Claudia; Hume, Michael; Nisbet, David; Afanador-Téllez, German; Van Kley, Alexandra Martynova; Nalian, Armen

    2014-10-01

    Essential oils have been proposed as alternatives to antibiotic use in food animal production. This study evaluated 3 chemotypes of the Origanum genus, containing varying amounts of secondary metabolites carvacrol, thymol, and sabinene, in the broiler chicken diet. Aerial parts of Origanum vulgare L. (OL), O. vulgare L. ssp. hirtum (OH), and O. majorana (OM) were collected from a greenhouse located in the high altitude Sabana de Bogotá (Savanna of Bogotá) and O. vulgare L. ssp. hirtum (OG) produced and ground in Greece. Oregano essential oils (OEO) from these plants were obtained by steam distillation and analyzed by gas chromatography coupled to a mass spectrometer. Six treatments were evaluated: 200 mg/kg of OEO from OH, OL, and OM, 50 mg/kg of OEO from OG, 500 mg/kg of chlortetracycline, and without additives. Broiler chicks were maintained at 2,600 m above sea level, placed in brooder cages under a completely randomized design. Template DNA was isolated from duodenal, jejunal, ileal, and cecal contents in each group and bacterial 16S rDNA patterns were analyzed by denaturing gradient gel electrophoresis. Dendrograms of denaturing gradient gel electrophoresis band patterns revealed 2 main clusters, OEO-treated chicks and nontreated control chicks, in each intestinal segment. Band patterns from different gut compartments revealed major bacterial population shifts in the foregut (duodenum, jejunum, and ileum) compared with the hindgut (cecum and colon) at all ages evaluated (P < 0.05). The OEO groups showed less shift (62.7% similarity coefficient) between these 2 compartments versus the control groups (53.7% similarity coefficient). A reduction of 59% in mortality from ascites was seen in additive-supplemented groups compared with the control group. This study represents the first work to evaluate the effects of the 3 main chemotypes of Origanum genus in broilers.

  2. La scrittura musicale come prerogativa della composizione musicale occidentale

    Directory of Open Access Journals (Sweden)

    Manfred Hermann Schmid

    2012-11-01

    Full Text Available Nel mondo occidentale, saper scrivere signífica il massimo della competenza. Perfino nella percezione popolare il ruolo che in musica spetta alla scrittura è percepito come una sfida. Nel marzo 2010, in una trasmissione televisiva equivalente a “Scommettiamo che”, un anchorman tedesco ha chiesto ad Anna Netrebko, sua ospite, se nella carriera di una cantante sia necessario saper leggere perfettamente la musica. “No”, è stata la risposta, istantanea, “basta avere bella voce e buona memoria”. Accostatasi poi al pianoforte per intonare un Lied di Rimskij-Korsakov, mentre l’accompagnatrice apriva lo spartito, la Netrebko aggiungeva scherzosamente: il pianista sì che deve saper leggere la musica! Per l’esperto, questa maliziosa risposta a doppio senso richiama l’antica distinzione erudita tra sapere pratico e teorico, rappresentati rispettivamente dalla voce umana, di cui ciascuno dispone in natura, e da uno strumento tecnico tradizionalmente adibito alla teoria, erede del monocordo antico, sulla cui tastiera le divisioni della corda erano indicate con lettere alfabetiche: lettere che sono le primissime testimonianze di una scrittura musicale.

  3. Pollination efficiency and effectiveness or bumble bees and hummingbirds visiting Delphinium nelsonii

    Directory of Open Access Journals (Sweden)

    Waser, Nicholas M.

    1990-12-01

    Full Text Available Delphinium nelsonii Greene is a spring-flowering perennial of the Rocky Mountains of North America. Its blue flowers conform to a classical «bee pollination syndrome», but in western Colorado they are visited by hummingbirds (mostly in the first half of the flowering season as well as bumble bee queens (mostly in the second half of the season. Experiments with potted plants showed that a bee deposits about 10 times as much pollen while visiting a flower as does a bird, and causes about la times as many seeds to be set. In contrast, bees and birds appear similar in the «quality» of pollen they deliver, e. g., in its outcrossing distance. At the level of entire pollinator populations, hummingbird visitation rates may be over 10 times as great as those or bumble bees, in part because birds visit flowers more quickly. Thus the two visitor classes should deliver similar pollen quantities overall, which is confirmed by similar pollen loads of flowers early and late in the season, and should contribute about equally to seed set, which is confirmed by several experiments and observations. Exact relative contributions probably depend on pollinator population sizes, which in the case of hummingbirds appear to have varied 2.5 fold across 14 years. The similar contributions or birds and bees lo seed set shows that individual pollination efficiency must be distinguished from population-level effectiveness. and that the «pollination syndrome» of a flower may not indicate present-day effectiveness of its visitors.

    [ca] Delphinium nelsonii Greene és una planta perenne de floració primaveral que creix a les Muntanyes Rocoses de Nordamèrica. Les seves flors blaves s'acorden a la clàssica (síndrome de pol-linització per abellots», però, a l'oest de Colorado, són visitades per colibrís (durant la primera meitat del període de floració així com per reines d'abellots (principalment durant la segona meitat del període. Els

  4. First Phytochemical Evidence of Chemotypes for the Seagrass Zostera noltii

    Directory of Open Access Journals (Sweden)

    Micheline Grignon-Dubois

    2012-09-01

    Full Text Available The variability of the flavonoid content of two populations of Z. noltii from different geographical zones, i.e., the Bay of Arcachon and the Bay of Cadiz, was evaluated. Samples were collected in spring and autumn at the two sites, and extracts were prepared by maceration in water. The phenolic content was fully characterized using Nuclear Magnetic Resonance (NMR, UV and Liquid Chromatography-Mass Spectrometry (LC-MS, and the concentration of the individual phenolic was determined by quantitative High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD. The two populations show a strong geographical differentiation in their flavonoid content. The samples from Cadiz were dominated by apigenin 7-sulfate, which represents 71% (autumn collection and 83% (spring collection of the total flavonoids, whereas the samples from Arcachon were characterized by diosmetin 7-sulfate (85 and 93% of the total flavonoids. Structural elucidation of the individual phenolics was assigned using the complementary information from their spectral evidence. In addition, the results were confirmed by acid hydrolysis of the flavonoid sulfates, and comparison to synthetic standards obtained by sulfation of apigenin, diosmetin and luteolin. The results represent the first experimental evidence of the existence of chemotypes within the species Z. noltii.

  5. Cashew-nut (Anacardium occidentale L.) Drying Study Using a Solar Dryer with Direct Radiation

    OpenAIRE

    Machado, Antônio V.; Oliveira,Edson L. de; Santos, Everaldo S.; Jackson A de Oliveira

    2010-01-01

    MACHADO, Antônio V. et al. Estudio del Secado de Anacardo (Anacardium occidentale L.) mediante Secador Solar de Radiación Directa. Información Tecnológica, v. 21, n. 1, p. 31-37, 2010. Resumen: Se presentan ensayos de secado con radiación solar directa y su modelado matemático, para evaluar la cinética de secado del anacardo (Anacardium occidentale L.) y comparar su eficiencia en relación al secado solar natural en tablero. Los experimentos fueron realizados con rodajas de fruta de 1 cm. y...

  6. Isolation and Characterization of the Chemical Constituents of Anacardium occidentale Cracked Bark

    Directory of Open Access Journals (Sweden)

    O. E. Fadeyi *

    2015-06-01

    Full Text Available The cracked bark of Anacardium occidentale were dried under ambient conditions, chopped into bits and the ethanolic extract of the cracked bark was obtained by cold extraction. Phytochemical screening was conducted to identify the types of secondary metabolites present using standard procedures. The profiles of the chemical constituents present were established using Thin Layer and Column Chromatography methods. Thus, pure chemical constituents were isolated from the cracked bark of Anacardium occidentale. The isolated compounds were characterized using FT-IR and their structures determined using data obtained from GC-MS spectrum.

  7. Chemical composition and antimicrobial properties of different basil essential oils chemotypes from Togo

    Directory of Open Access Journals (Sweden)

    Koffi Koba

    2009-06-01

    Full Text Available The aerial parts essential oils of Ocimum basilicum (Lamiaceae from Togo were steam-distilled and investigated for their percentage composition (GC and GC/MS and in vitro antimicrobial activities. Five oil chemotypes were identified and classified as follows in line with their principal components: estragole type; linalool/estragole type; methyleugenol type; methyleugenol/t-anethole type; t-anethole type. The in vitro microbiological experiments revealed that only the methyleugenol and methyleugenol/t-anethole chemotypes were active against tested fungi and bacteria. Their minimum inhibitory concentrations (MIC ranged from 80 to 150 µl.l-1 and from 200 to 500 µl.l-1 respectively. Likewise, on tested bacteria the MIC varied from 200 µl.l-1 to 400 µl.l-1 and from 250 to 500 µl.l-1 respectively. These findings are supportive of the potential of both basil oil chemotypes for use as active ingredients in natural antibiotic drugs.

  8. Evolution of the Cannabinoid and Terpene Content during the Growth of Cannabis sativa Plants from Different Chemotypes.

    Science.gov (United States)

    Aizpurua-Olaizola, Oier; Soydaner, Umut; Öztürk, Ekin; Schibano, Daniele; Simsir, Yilmaz; Navarro, Patricia; Etxebarria, Nestor; Usobiaga, Aresatz

    2016-02-26

    The evolution of major cannabinoids and terpenes during the growth of Cannabis sativa plants was studied. In this work, seven different plants were selected: three each from chemotypes I and III and one from chemotype II. Fifty clones of each mother plant were grown indoors under controlled conditions. Every week, three plants from each variety were cut and dried, and the leaves and flowers were analyzed separately. Eight major cannabinoids were analyzed via HPLC-DAD, and 28 terpenes were quantified using GC-FID and verified via GC-MS. The chemotypes of the plants, as defined by the tetrahydrocannabinolic acid/cannabidiolic acid (THCA/CBDA) ratio, were clear from the beginning and stable during growth. The concentrations of the major cannabinoids and terpenes were determined, and different patterns were found among the chemotypes. In particular, the plants from chemotypes II and III needed more time to reach peak production of THCA, CBDA, and monoterpenes. Differences in the cannabigerolic acid development among the different chemotypes and between monoterpene and sesquiterpene evolution patterns were also observed. Plants of different chemotypes were clearly differentiated by their terpene content, and characteristic terpenes of each chemotype were identified.

  9. Evolution of the Cannabinoid and Terpene Content during the Growth of Cannabis sativa Plants from Different Chemotypes

    NARCIS (Netherlands)

    Aizpurua-Olaizola, Oier; Soydaner, Umut; Öztürk, Ekin; Schibano, Daniele; Simsir, Yilmaz; Navarro, Patricia; Etxebarria, Nestor; Usobiaga, Aresatz

    2016-01-01

    The evolution of major cannabinoids and terpenes during the growth of Cannabis sativa plants was studied. In this work, seven different plants were selected: three each from chemotypes I and III and one from chemotype II. Fifty clones of each mother plant were grown indoors under controlled

  10. Chemotype diversity of indigenous Dalmatian sage (Salvia officinalis L.) populations in Montenegro.

    Science.gov (United States)

    Stešević, Danijela; Ristić, Mihailo; Nikolić, Vuko; Nedović, Marijana; Caković, Danka; Šatović, Zlatko

    2014-01-01

    To identify how many chemotypes of Salvia officinalis exist in Montenegro, the chemical composition of the essential oils of 12 wild-growing populations was determined by GC-FID and GC/MS analyses. Among the 40 identified constituents, the most abundant were cis-thujone (16.98-40.35%), camphor (12.75-35.37%), 1,8-cineol (6.40-12.06%), trans-thujone (1.5-10.35%), camphene (2.26-9.97%), borneol (0.97-8.81%), viridiflorol (3.46-7.8%), limonene (1.8-6.47%), α-pinene (1.59-5.46%), and α-humulene (1.77-5.02%). The composition of the essential oils under study did not meet the ISO 9909 requirements, while the oils of populations P02-P04, P09, and P10 complied with the German Drug Codex. A few of the main essential-oil constituents appeared to be highly intercorrelated. Strong positive correlations were observed between α-pinene and camphene, camphene and camphor, as well as between cis-thujone and trans-thujone. Strong negative correlations were evidenced between cis-thujone and α-pinene, cis-thujone and champhene, cis-thujone and camphor, as well as between trans-thujone and camphene. Multivariate analyses allowed the grouping of the populations into three distinct chemotypes, i.e., Chemotype A, rich in total thujones, Chemotype B, with intermediate contents of thujones, α-pinene, camphene, and camphor and high borneol contents, and Chemotype C, rich in camphor, camphene, and α-pinene. The chemotypes did not significantly differ in the total essential-oil content and the cis/trans-thujone ratio.

  11. Isolation and characterization of polyphenol oxidase from Sardinian poisonous and non-poisonous chemotypes of Ferula communis (L.).

    Science.gov (United States)

    Zucca, Paolo; Sanjust, Enrico; Loi, Martina; Sollai, Francesca; Ballero, Mauro; Pintus, Manuela; Rescigno, Antonio

    2013-06-01

    Ferula communis (L.), a plant belonging to Apiaceae, is widely present in Sardinia, Italy. Currently, interest in F. communis focuses on the presence of two chemotypes in the wild. One chemotype is poisonous to animals, whereas the other chemotype is non-poisonous. Polyphenol oxidase (PPO) has been extracted and partially purified from the two chemotypes of F. communis. The biochemical characterization of the enzymes showed significant differences. In particular, while the two PPOs were not able to use 6- and 7-hydroxycoumarin as substrates, they showed distinct specificity for 6,7- and 7,8-dihydroxycoumarin. Significant differences in the enzyme behavior towards common PPO inhibitors were also observed. In addition, activation energy and activation energy for denaturation were determined, showing significant differences between FP-PPO and FNP-PPO, particularly for denaturation kinetics. The possible roles of the two PPOs in determining differences in composition and toxicity of the two F. communis chemotypes are also discussed.

  12. Identification of the chemotypes of Ocimum forskolei and Ocimum basilicum by NMR spectroscopy.

    Science.gov (United States)

    Fatope, Majekodunmi O; Marwah, Ruchi G; Al Hadhrami, Nabil M; Onifade, Anthony K; Williams, John R

    2008-11-01

    The chemotypes of Ocimum forskolei Benth and Ocimum basilicum L. growing wild in Oman have been established by (13)C-NMR analyses of the vegetative and floral oils of the plants. The chemotypes, estragole for O. forskolei and linalool for O. basilicum, suggested by (13)C-NMR fingerprinting were also confirmed by GC-FID and GC/MS analyses. The oil of O. forskolei demonstrated better activities against bacteria and dermatophytes. The significance of the presence of estragole and linalool in the volatile oils of plants whose fragrances are traditionally inhaled, added to food, or rubbed on the skin are discussed.

  13. The relationship between genetic and chemotypic diversity in American ginseng (Panax quinquefolius L.).

    Science.gov (United States)

    Schlag, Erin M; McIntosh, Marla S

    2013-09-01

    Ginseng is one of the world's most important herbals used as an adaptogen and a cure for an impressively large range of ailments. Differences in the medicinal properties of ginseng roots have been attributed to variation in ginsenoside composition. In this study, the association between genetic and chemotypic profiles of wild and cultivated American ginseng (Panax quinquefolius L.) roots grown in Maryland was investigated. Ginseng roots were classified into chemotypes based on their relative composition of Re and Rg1. Genetic profiles of these roots were determined from the analysis of 38 polymorphic RAPD markers and used for a cluster analysis of genetic similarities. The close correspondence between chemotype and genetic cluster provides the first DNA-based evidence for the genetic basis of ginsenoside composition. Results of this research are significant for plant breeding and conservation, phytochemical research, and clinical and pharmacological studies. Also, the correlation between RAPD markers and chemotype indicates the potential to use RAPD markers as a reliable and practical method for identification and certification of ginseng roots. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. The Effect of Co-Administration of Death Camas (Zigadenus spp.) and Low Larkspur (Delphinium spp.) in Cattle.

    Science.gov (United States)

    Welch, Kevin D; Green, Benedict T; Gardner, Dale R; Stonecipher, Clinton A; Pfister, James A; Cook, Daniel

    2016-01-12

    In many rangeland settings, there is more than one potential poisonous plant. Two poisonous plants that are often found growing simultaneously in the same location in North American rangelands are death camas (Zigadenus spp.) and low larkspur (Delphinium spp.). The objective of this study was to determine if co-administration of death camas would exacerbate the toxicity of low larkspur in cattle. Cattle dosed with 2.0 g of death camas/kg BW showed slight frothing and lethargy, whereas cattle dosed with both death camas and low larkspur showed increased clinical signs of poisoning. Although qualitative differences in clinical signs of intoxication in cattle co-treated with death camas and low larkspur were observed, there were not any significant quantitative differences in heart rate or exercise-induced muscle fatigue. Co-treatment with death camas and low larkspur did not affect the serum zygacine kinetics, however, there was a difference in the larkspur alkaloid kinetics in the co-exposure group. Overall, the results from this study suggest that co-exposure to death camas and low larkspur is not significantly more toxic to cattle than exposure to the plants individually. The results from this study increase our knowledge and understanding regarding the acute toxicity of death camas and low larkspur in cattle.

  15. Limited Stability of Microcystins in Oligopeptide Compositions of Microcystis aeruginosa (Cyanobacteria: Implications in the Definition of Chemotypes

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    Ramsy Agha

    2013-06-01

    Full Text Available The occurrence of diverse oligopeptides in cyanobacteria, including the cyanotoxins microcystins, has been recently used to classify individual clones into sub-specific oligopeptide chemotypes, whose composition and dynamics modulate microcystin concentrations in cyanobacterial blooms. Cyanobacterial chemotyping allows the study of the ecology of chemotypical subpopulations, which have been shown to possess dissimilar ecological traits. However, the stability of chemotypes under changing abiotic conditions is usually assumed and has not been assessed in detail. We monitored oligopeptide patterns of three strains of Microcystis aeruginosa under different nutrient and light conditions. MALDI-TOF MS revealed alterations in the microcystins signatures under N and P poor conditions and high light intensities (150 and 400 μmol photons m−2s−1. Variations in the general oligopeptide composition were caused by a gradual disappearance of microcystins with low relative intensity signals from the fingerprint. The extent of such variations seems to be closely related to physiological stress caused by treatments. Under identical clonal compositions, alterations in the oligopeptide fingerprint may be misinterpreted as apparent shifts in chemotype succession. We discuss the nature of such variations, as well as the consequent implications in the use of cyanobacterial chemotyping in studies at the subpopulation level and propose new guidance for the definition of chemotypes as a consistent subpopulation marker.

  16. Analytical discrimination of poisonous and nonpoisonous chemotypes of giant fennel (Ferula communis L.) through their biologically active and volatile fractions.

    Science.gov (United States)

    Rubiolo, Patrizia; Matteodo, Maura; Riccio, Giovanna; Ballero, Mauro; Christen, Philippe; Fleury-Souverain, Sandrine; Veuthey, Jean-Luc; Bicchi, Carlo

    2006-10-04

    Giant fennel (Ferula communis L.) from Sardinia is characterized by two chemotypes with different biological activities. One chemotype is poisonous, due to prenylcoumarins, and responsible for ferulosis, which mainly affects sheep and goats, cattle, and horses; the other chemotype is nonpoisonous and contains daucane esters. The two chemotypes cannot be distinguished botanically. High-performance liquid chromatography-diode array-ultraviolet detection-mass spectrometry (HPLC-DAD-UV-MS) analysis of the composition of the fractions containing the biologically active metabolites and of the volatile fractions, by gas chromatography-mass spectrometry (GC-MS), of both essential oil and headspace sampled by headspace solid-phase microextraction (HS-SPME) are here shown to be effective in discriminating the poisonous and nonpoisonous chemotypes. HS-SPME with CAR/PDMS/DVB in combination with GC-MS has also been found to be a successful, fully automated one-step method for rapid and unequivocal discrimination of the two chemotypes, using aristolene and allohedycaryol as markers of the poisonous and nonpoisonous chemotypes, respectively.

  17. Molecular and phenotypic descriptions of Stachybotrys chlorohalonata sp. nov. and two chemotypes of Stachybotrys chartarum found in water-damaged buildings.

    Science.gov (United States)

    Andersen, Birgitte; Nielsen, Kristian F; Thrane, Ulf; Szaro, Tim; Taylor, John W; Jarvis, Bruce B

    2003-01-01

    Twenty-five Stachybotrys isolates from two previous studies have been examined and compared, using morphological, chemical and phylogenetic methods. The results show that S. chartarum sensu lato can be segregated into two chemotypes and one new species. The new species, S. chlorohalonata, differs morphologically from S. chartarum by having smooth conidia, being more restricted in growth and producing a green extracellular pigment on the medium CYA. S. chlorohalonata and S. chartarum also have different tri5, chs1 and tub1 gene fragment sequences. The two chemotypes of S. chartarum, chemotype S and chemotype A, have similar morphology but differ in production of metabolites. Chemotype S produces macrocyclic trichothecenes, satratoxins and roridins, while chemotype A produces atranones and dolabellanes. There is no difference between the two chemotypes in the tub1 gene fragment, but there is a one nucleotide difference in each of the tri5 and the chs1 gene fragments.

  18. Lipids Characterization and Industrial Potentials of Pumpkin Seeds (Telfairia occidentalis and Cashew Nuts (Anacardium occidentale

    Directory of Open Access Journals (Sweden)

    E. O. Eddy

    2011-01-01

    Full Text Available Oil from Telfaria occidentalis and Anarcardium occidentale has been extracted and characterized. The lipid content of the Telfaria occidentalis and Anarcardium occidentalis were 58.41% and 42.15% respectively. The physicochemical parameters of Telfaria occidentalis and Anarcardium occidentale seeds were; boiling point; (58.90, 62.60 °C, melting point; (18.50, 21.80 °C, refractive index; (1.462, 1.498, specific gravity; (0.87, 0.69 saponification value; (91.16, 92.57 iodine value; (51.52, 47.20, acid value; (0.76, 3.74 ester value; (90.40, 88.87, % free fatty acid; (.38, 1.88 and peroxide value; (11.75, 15.23 respectively. Oils from these seeds were found to exhibit the needed potentials for utilization in paint and food industries and as biofuel.

  19. Insect fauna associated with Anacardium occidentale (Sapindales: Anacardiaceae) in Benin, West Africa.

    OpenAIRE

    Agboton, C.; Onzo, A.; Ouessou, F. I.; Goergen, G.; Vidal, S; Tamò, M.

    2014-01-01

    Cashew, Anacardium occidentale L. (Sapindales: Anacardiaceae), is an important cash crop in Benin. However, its production is threatened by several biotic factors, especially insects. In Benin, very few studies have focused on insects and just listed species commonly found on cashew worldwide. The present investigation fills this gap by presenting an exhaustive inventory of insect species associated with this crop in the country. The survey was carried out from September 2009 to August 2010 i...

  20. Reductive-degradation of carcinogenic azo dyes using Anacardium occidentale testa derived silver nanoparticles.

    Science.gov (United States)

    Edison, Thomas Nesakumar Jebakumar Immanuel; Atchudan, Raji; Sethuraman, Mathur Gopalakrishnan; Lee, Yong Rok

    2016-09-01

    In the present work, reductive-degradation of azo dyes such as congo red (CR) and methyl orange (MO) was manifested using Anacardium occidentale testa derived silver nanoparticles (AgNPs) as a catalyst. The formation of highly stable AgNPs were visually confirmed by the appearance of yellow color and further substantiated by the existence of surface plasmon resonance (SPR) peak around 425nm. The effect of A. occidentale concentration, reaction time and pH in the formations of AgNPs was corroborated by UV-visible (UV-Vis) spectroscopy. The Fourier transform infrared (FT-IR) spectroscopic results proved that phytoconstituents of A. occidentale testa acts as a capping agent and thereby protects the AgNPs from aggregation. The crystalline nature of the AgNPs was validated from the XRD patterns. The average size of synthesized AgNPs was 25nm, with distorted spherical shape was ascribed from the high resolution transmission electron microscopic (HR-TEM) images. Due to the high stability of the as-synthesized AgNPs, they were utilized for the degradation of carcinogenic azo dyes such as CR and MO using NaBH4 and its catalytic activity was studied via UV-Vis spectroscopy. The results proved that extraordinary catalytic activity of synthesized AgNPs towards the reductive-degradation of both CR and MO.

  1. Interaction of gamma radiation on the functionality of the molluscicidal extract of Anacardium occidentale Linn

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Gustavo Henrique Farias dos [Grupo de Estudos em Radioprotecao e Radioecologia (GERAR) . Departamento de Energia Nuclear. Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil); Silva, Edvane Borges da [Centro Academico de Vitoria. Universidade Federal de Pernambuco (UFPE), Vitoria de Santo Antao, PE (Brazil); Amorim, Elba Lucia C.; Peixoto Sobrinho, Tadeu J.S., E-mail: elba@ufpe.br [Departamento de Ciencias Farmaceuticas. Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil); Melo, Ana Maria Mendonca de Albuquerque; Lima, Claudia Sampaio de Andrade [Departamento de Biofisica e Radiobiologia. Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2011-07-01

    Biological control of Biomphalaria glabrata that is the intermediate host of Schistosoma mansoni, through molluscicides has been an alternative against schistosomiasis. Many studies have been developed to obtain molluscicide products, from plants. Anacardium occidentale L. (cashew), a plant rich in phenolic compounds shows molluscicidal activity in earlier assays. However there is an interest of enhancing the action of bioactive substances in order to use it in small concentrations, reducing costs in their utilization. This study were conducted using ethanolic extracts of bark and leaves of A. occidentale, before and after exposure to gamma radiation from {sup 60}Co, checking their secondary metabolites, their biological activity against Biomphalaria glabrata and environmental toxicity. The extracts of A. occidentale were obtained by cold maceration in 70% ethanol, filtered, dried and divided into two experimental groups: control (0 kGy) and irradiated at a dose of 10 kGy. The quantification of metabolites was performed in six replicates for the determination of total phenols by Folin-Ciocalteau method and tannins, the precipitation of casein. Tests for biological control of embryos and adults of B. glabrata snails and assessment of environmental toxicity (using Artemia salina larvae) were performed in triplicate, following a period of 24 hours of exposure to extracts at a concentration of 100 mg/L and its respective controls. Data were expressed as percentages of means and standard deviations. The results showed that the gamma radiation from {sup 60}Co resulted in leaf extracts, increased levels of total phenols and tannins, which enhanced the lethality for embryos and adults of B. glabrata. There was a reduction of the toxicity of leaf extracts after irradiation in the Artemia salina. Since in extracts of bark, gamma radiation did not alter the levels of total phenols and tannins, however, it was noted potentiation of lethality of adult snails of B. glabrata. The

  2. Discovery of new antimalarial chemotypes through chemical methodology and library development.

    Science.gov (United States)

    Brown, Lauren E; Chih-Chien Cheng, Ken; Wei, Wan-Guo; Yuan, Pingwei; Dai, Peng; Trilles, Richard; Ni, Feng; Yuan, Jing; MacArthur, Ryan; Guha, Rajarshi; Johnson, Ronald L; Su, Xin-zhuan; Dominguez, Melissa M; Snyder, John K; Beeler, Aaron B; Schaus, Scott E; Inglese, James; Porco, John A

    2011-04-26

    In an effort to expand the stereochemical and structural complexity of chemical libraries used in drug discovery, the Center for Chemical Methodology and Library Development at Boston University has established an infrastructure to translate methodologies accessing diverse chemotypes into arrayed libraries for biological evaluation. In a collaborative effort, the NIH Chemical Genomics Center determined IC(50)'s for Plasmodium falciparum viability for each of 2,070 members of the CMLD-BU compound collection using quantitative high-throughput screening across five parasite lines of distinct geographic origin. Three compound classes displaying either differential or comprehensive antimalarial activity across the lines were identified, and the nascent structure activity relationships (SAR) from this experiment used to initiate optimization of these chemotypes for further development.

  3. Ligand-Based Virtual Screening in a Search for Novel Anti-HIV-1 Chemotypes.

    Science.gov (United States)

    Kurczyk, Agata; Warszycki, Dawid; Musiol, Robert; Kafel, Rafał; Bojarski, Andrzej J; Polanski, Jaroslaw

    2015-10-26

    In a search for new anti-HIV-1 chemotypes, we developed a multistep ligand-based virtual screening (VS) protocol combining machine learning (ML) methods with the privileged structures (PS) concept. In its learning step, the VS protocol was based on HIV integrase (IN) inhibitors fetched from the ChEMBL database. The performances of various ML methods and PS weighting scheme were evaluated and applied as VS filtering criteria. Finally, a database of 1.5 million commercially available compounds was virtually screened using a multistep ligand-based cascade, and 13 selected unique structures were tested by measuring the inhibition of HIV replication in infected cells. This approach resulted in the discovery of two novel chemotypes with moderate antiretroviral activity, that, together with their topological diversity, make them good candidates as lead structures for future optimization.

  4. The geographic distribution and complex evolutionary history of the NX-2 trichothecene chemotype from Fusarium graminearum.

    Science.gov (United States)

    Kelly, Amy; Proctor, Robert H; Belzile, Francois; Chulze, Sofia N; Clear, Randall M; Cowger, Christina; Elmer, Wade; Lee, Theresa; Obanor, Friday; Waalwijk, Cees; Ward, Todd J

    2016-10-01

    Fusarium graminearum and 21 related species comprising the F. sambucinum species complex lineage 1 (FSAMSC-1) are the most important Fusarium Head Blight pathogens of cereal crops world-wide. FSAMSC-1 species typically produce type B trichothecenes. However, some F. graminearum strains were recently found to produce a novel type A trichothecene (NX-2) resulting from functional variation in the trichothecene biosynthetic enzyme Tri1. We used a PCR-RFLP assay targeting the TRI1 gene to identify the NX-2 allele among a global collection of 2515 F. graminearum. NX-2 isolates were only found in southern Canada and the northern U.S., where they were observed at low frequency (1.8%), but over a broader geographic range and set of cereal hosts than previously recognized. Phylogenetic analyses of TRI1 and adjacent genes produced gene trees that were incongruent with the history of species divergence within FSAMSC-1, indicating trans-species evolution of ancestral polymorphism. In addition, placement of NX-2 strains in the TRI1 gene tree was influenced by the accumulation of nonsynonymous substitutions associated with the evolution of the NX-2 chemotype, and a significant (PNX-2 branch (ω=1.16) in comparison to other branches (ω=0.17) in the TRI1 phylogeny. Parameter estimates were consistent with positive selection for specific amino-acid changes during the evolution of NX-2, but direct tests of positive selection were not significant. Phylogenetic analyses of fourfold degenerate sites and intron sequences in TRI1 indicated the NX-2 chemotype had a single evolutionary origin and evolved recently from a type B ancestor. Our results indicate the NX-2 chemotype may be indigenous, and possibly endemic, to southern Canada and the northern U.S. In addition, we demonstrate that the evolution of TRI1 within FSAMSC-1 has been complex, with evidence of trans-species evolution and chemotype-specific shifts in selective constraint. Published by Elsevier Inc.

  5. Chemical Composition and Antimicrobial Activity of a New Chemotype of Hyptis suaveolens (Poit from Nigeria

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    B.A. Iwalokun

    2012-04-01

    Full Text Available Hyptis suaveolens is one of the aromatic plants credited for substantial medicinal values in the tropics with three chemotypes previously reported in Nigeria. This study provided biological and chemical evidence for a new chemotype of Hyptis suaveolens in Lagos. Hydrodistillation of the dried leaves of the plant produced volatile oil with a yield of 0.31% and subsequent analyses by GC-MS identified 28 volatile compounds that accounted for 99.1% of the total oil composition. Although the oil was monoterpenoid dominated and has comparable levels of sabinene (25.8 vs. 13.2-30.1%, a-thujene (1.1 vs. 0.9-1.2%, and 4- terpineol (8.4-9.8 vs. 11.4%, it elicited a moderate level of α-pinene (4.7 vs. 1.8-13.6, higher levels of β- pinene (9.7 vs. 0-4.4%, limonene (2.3 vs. 0-0.8%, 1,8-cineole (4.8 vs. 0-1.2%, γ-terpinene (9.3 vs. 1.6-4.2% and terpinolene (8.4 vs. 5.6-6.3 and the presence of new compounds: aromadendrene (0.3%, camphor (0.3%, germacrene B (0.4% and himachalol (0.1% when compared with the previous chemotypes. In vitro, the oil was found by agar diffusion assay to elicit antibacterial activity against E. coli ATCC25922, and S. aureus ATCC25923 and antifungal activity with C. albicans showing higher sensitivity (MFC = 53.3 μL/mL and Aspergillus niger and Trichophyton rubrum displaying moderate to low sensitivity. Biological effect of the oil at sub-MIC on E. coli ATCC25922 was characterized by dose-dependent loss of outer membrane proteins. These findings provide evidence for a new chemotype of Hyptis suaveolens in Nigeria.

  6. Intensive sampling identifies previously unknown chemotypes, population divergence and biosynthetic connections among terpenoids in Eucalyptus tricarpa.

    Science.gov (United States)

    Andrew, Rose L; Keszei, Andras; Foley, William J

    2013-10-01

    Australian members of the Myrtaceae produce large quantities of ecologically and economically important terpenes and display abundant diversity in both yield and composition of their oils. In a survey of the concentrations of leaf terpenes in Eucalyptus tricarpa (L.A.S. Johnson) L.A.S. Johnson & K.D. Hill, which were previously known from few samples, exceptional variability was found in composition. The aim was to characterize the patterns of variation and covariation among terpene components in this species and to use this information to enhance our understanding of their biosynthesis. There were marked discontinuities in the distributions of numerous compounds, including the overall proportions of mono- and sesquiterpenes, leading us to delineate three distinct chemotypes. Overall, positive covariation predominated, but negative covariation suggested competitive interactions involved in monoterpene synthesis. Two groups of covarying monoterpenes were found, each of which was positively correlated with a group of sesquiterpenes and negatively correlated with the alternate sesquiterpene group. These results imply substantial cross-talk between mono- and sesquiterpene biosynthesis pathways. However, only those compounds hypothesized to share final carbocation intermediates or post-processing steps were strongly positively correlated within chemotypes. This suggests that the broader patterns of covariation among groups of compounds may result from co-regulation of multiple biosynthetic genes, controlling the complex terpene profiles of the chemotypes of Eucalyptus.

  7. The genetic basis for 3-ADON and 15-ADON trichothecene chemotypes in Fusarium.

    Science.gov (United States)

    Alexander, Nancy J; McCormick, Susan P; Waalwijk, Cees; van der Lee, Theo; Proctor, Robert H

    2011-05-01

    Certain Fusarium species cause head blight of wheat and other small grains worldwide and produce trichothecene mycotoxins. These mycotoxins can induce toxicoses in animals and humans and can contribute to the ability of some fusaria to cause plant disease. Production of the trichothecene 3-acetyldeoxynivalenol (3-ADON) versus 15-acetyldeoxynivalenol (15-ADON) is an important phenotypic difference within and among some Fusarium species. However, until now, the genetic basis for this difference in chemotype has not been identified. Here, we identified consistent DNA sequence differences in the coding region of the trichothecene biosynthetic gene TRI8 in 3-ADON and 15-ADON strains. Functional analyses of the TRI8 enzyme (Tri8) in F. graminearum, the predominant cause of wheat head blight in North America and Europe, revealed that Tri8 from 3-ADON strains catalyzes deacetylation of the trichothecene biosynthetic intermediate 3,15-diacetyldeoxynivalenol at carbon 15 to yield 3-ADON, whereas Tri8 from 15-ADON strains catalyzes deacetylation of 3,15-diacetyldeoxynivalenol at carbon 3 to yield 15-ADON. Fusarium strains that produce the trichothecene nivalenol have a Tri8 that functions like that in 15-ADON strains. TRI3, which encodes a trichothecene carbon 15 acetyltransferase, was found to be functional in all three chemotypes. Together, our data indicate that differential activity of Tri8 determines the 3-ADON and 15-ADON chemotypes in Fusarium.

  8. Genetic Divergence and Chemotype Diversity in the Fusarium Head Blight Pathogen Fusarium poae

    Directory of Open Access Journals (Sweden)

    Adriaan Vanheule

    2017-08-01

    Full Text Available Fusarium head blight is a disease caused by a complex of Fusarium species. F. poae is omnipresent throughout Europe in spite of its low virulence. In this study, we assessed a geographically diverse collection of F. poae isolates for its genetic diversity using AFLP (Amplified Fragment Length Polymorphism. Furthermore, studying the mating type locus and chromosomal insertions, we identified hallmarks of both sexual recombination and clonal spread of successful genotypes in the population. Despite the large genetic variation found, all F. poae isolates possess the nivalenol chemotype based on Tri7 sequence analysis. Nevertheless, Tri gene clusters showed two layers of genetic variability. Firstly, the Tri1 locus was highly variable with mostly synonymous mutations and mutations in introns pointing to a strong purifying selection pressure. Secondly, in a subset of isolates, the main trichothecene gene cluster was invaded by a transposable element between Tri5 and Tri6. To investigate the impact of these variations on the phenotypic chemotype, mycotoxin production was assessed on artificial medium. Complex blends of type A and type B trichothecenes were produced but neither genetic variability in the Tri genes nor variability in the genome or geography accounted for the divergence in trichothecene production. In view of its complex chemotype, it will be of utmost interest to uncover the role of trichothecenes in virulence, spread and survival of F. poae.

  9. Sub-chronic hepatotoxicity of Anacardium occidentale (Anacardiaceae inner stem bark extract in rats

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    Okonkwo T. J. N.

    2010-01-01

    Full Text Available The extracts of Anacardium occidentale have been used in the management of different cardiovascular disorders in Nigeria. These have necessitated the assessment of the toxicity of this plant extract in sub-chronic administration. The inner stem bark of Anacardium occidentale was extracted with 80 % methanol and quantitatively analysed for antinutrients and some heavy metals. The phytochemical compositions and acute toxicity of the extract were determined also. Toxicity profiles of the extract on some liver function parameters were evaluated following a sub-chronic oral administration at doses of 1.44 and 2.87 g/kg. The phytochemical screening of extract revealed the presence of high amount of tannins, moderate saponins and trace of free reducing sugars. The antinutrient levels were 5.75 % (tannins, 2.50 % (oxalates, 2.00 % (saponins, 0.25 % (phytate and 0.03 % (cyanide. The quantity of iron detected from dried crude was 8.92 mg/100 g, while lead and cadmium were non-detectable. The extract had LD 50 of 2.154 g/kg p.o. in mice. Sub-chronic administration of the extract significantly increased the serum levels of alanine aminotransaminase and aspartate aminotransaminase, which are indicative of liver damage. The serum levels of alkaline phosphatase and total protein of the treated animals were not significantly increased. The effects of sub-chronically administered extract on hepatocytes were minimal as the serum alkaline phosphatase; total bilirubin and total protein levels in treated animals were not significant (p< 0.05. Thus, sub-chronic administrations of Anacardium occidentale inner stem bark extract did not significantly (p< 0.05 depress the function of hepatocytes in Wistar rats.

  10. Sub-chronic Hepatotoxicity of Anacardium occidentale (Anacardiaceae) Inner Stem Bark Extract in Rats.

    Science.gov (United States)

    Okonkwo, T J N; Okorie, O; Okonta, J M; Okonkwo, C J

    2010-05-01

    The extracts of Anacardium occidentale have been used in the management of different cardiovascular disorders in Nigeria. These have necessitated the assessment of the toxicity of this plant extract in sub-chronic administration. The inner stem bark of Anacardium occidentale was extracted with 80 % methanol and quantitatively analysed for antinutrients and some heavy metals. The phytochemical compositions and acute toxicity of the extract were determined also. Toxicity profiles of the extract on some liver function parameters were evaluated following a sub-chronic oral administration at doses of 1.44 and 2.87 g/kg. The phytochemical screening of extract revealed the presence of high amount of tannins, moderate saponins and trace of free reducing sugars. The antinutrient levels were 5.75 % (tannins), 2.50 % (oxalates), 2.00 % (saponins), 0.25 % (phytate) and 0.03 % (cyanide). The quantity of iron detected from dried crude was 8.92 mg/100 g, while lead and cadmium were non-detectable. The extract had LD(50)of 2.154g/kg p.o. in mice. Sub-chronic administration of the extract significantly increased the serum levels of alanine aminotransaminase and aspartate aminotransaminase, which are indicative of liver damage. The serum levels of alkaline phosphatase and total protein of the treated animals were not significantly increased. The effects of sub-chronically administered extract on hepatocytes were minimal as the serum alkaline phosphatase; total bilirubin and total protein levels in treated animals were not significant (p< 0.05). Thus, sub-chronic administrations of Anacardium occidentale inner stem bark extract did not significantly (p< 0.05) depress the function of hepatocytes in Wistar rats.

  11. Geografía, café y prosperidad en los andes occidentales de Colombia

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    Juan Barón

    2011-07-01

    Full Text Available Compuesta por los departamentos de Antioquia, Caldas, Quindío, Risaralda y Valle del Cauca, los Andes Occidentales ha sido por mucho años una de las regiones más prósperas de Colombia. Esta prosperidad se ha traducido en condiciones de vida e infraestructura superiores a las del resto del país. El éxito y bienestar que presentan hoy en día los Andes Occidentales están estrechamente ligados a la concentración de la producción de café, que se dio allí durante gran parte del siglo XX. El arraigo del café en la región no sólo se dio debido a que la geografía ofrecía las condiciones ideales para el cultivo, sino también a las instituciones cafeteras creadas para organizar la industria del café. A pesar de esta prosperidad, el constante descenso del precio internacional del café después del rompimiento del pacto de cuotas de producción en 1989, sumado al estancamiento de la industria manufacturera en algunos departamentos de la región, han afectado las economías departamentales menos diversificadas. Es así como la región de los Andes Occidentales Colombianos presenta las tasas de desempleo más altas del país, tasas que se han visto afectadas aún más con la crisis financiera internacional a través del menor flujo de remesas que los trabajadores oriundos de la región, y residentes en el exterior envían a sus familias.

  12. Novel method for isolation of major phenolic constituents from cashew (Anacardium occidentale L.) nut shell liquid.

    Science.gov (United States)

    Paramashivappa, R; Kumar, P P; Vithayathil, P J; Rao, A S

    2001-05-01

    Commercially available cashew (Anacardium occidentale L.) nut shell liquid (CNSL) mainly contains the phenolic constituents anacardic acid, cardol, and cardanol. These phenolic constituents are themselves heterogeneous, and each of them contains saturated, monoene, diene, and trienes in the fifteen-carbon side chain. This communication describes the separation of anacardic acid, cardol, and cardanol for industrial application. Anacardic acid was selectively isolated as calcium anacardate. The acid-free CNSL was treated with liquor ammonia and extracted with hexane/ethyl acetate (98:2) to separate the mono phenolic component, cardanol. Subsequently, ammonia solution was extracted with ethyl acetate/hexane (80:20) to obtain cardol.

  13. Process for isolation of cardanol from technical cashew (Anacardium occidentale L.) nut shell liquid.

    Science.gov (United States)

    Phani Kumar, P; Paramashivappa, R; Vithayathil, P J; Subba Rao, P V; Srinivasa Rao, A

    2002-07-31

    Commercially available technical cashew (Anacardium occidentale L.) nut shell liquid (CNSL) contains mainly cardanol (decarboxylated anacardic acid) and cardol. Cardanol, the monophenolic component of technical CNSL, is widely used as a synthon for the preparation of a number of polymers and agricultural products. This paper describes the separation of cardanol from toxic cardol. Technical CNSL was dissolved in a mixture of methanol and ammonium hydroxide (8:5) and extracted with hexane to obtain cardanol. The resultant methanolic ammonia layer was extracted with a mixture of ethyl acetate and hexane to yield cardol. This is the first industrially feasible process based on solvent extractions for the isolation of cardanol from technical CNSL.

  14. LA DIFFERENCE ENTRE LA CULTURE CHINOISE ET OCCIDENTALE ET LEURS INFLUENCES PROFONDES

    Institute of Scientific and Technical Information of China (English)

    俞渊善

    2014-01-01

    Cet essai vise à présenter les caractères de la culture Chinoise et Occidentale, ainsi que leur propre appartenance en traitant le contexte de formation et le processus d’évolution. Il est aussi consacré dans un but d’approfondissement d’étude de cette différence entre les deux cultures en mettant en exergue cette profonde signification sur les aspects de civilisation ainsi que sur les impacts sur l’économie, la langue et la situation sociale en général.

  15. LA COMPARAISON DE LA MUSIQUE TURQUE ET OCCIDENTALE EN MATIERE DE GAMME, RYTHME ET TONALITE

    Directory of Open Access Journals (Sweden)

    Kubilay Kolukirik

    2012-12-01

    Full Text Available Après avoir fini mon Doctorat en musique à l’université d’Ankara en Turquie, j’ai travaillé en tant que Maitre de conférences à l’université de Nevşehir. J’ai alors été contacté par le Conservatoire Claude Debussy, situé dans le 17eme arrondissement de Paris, afin d’effectuer une recherche comparative sur le Jazz et la musique folklorique Turque. Dans ce contexte j'ai participé aux cours de jazz de Philippe Poussard avec mon instrument authentique turc nommé "bağlama". Avec ce dernier nous avons joué des chansons folkloriques Turques, et j'ai ressenti que lui et ses étudiants ont été impressionnés par la musique que nous avons jouée ensemble, enimprovisant. Nous avons ensuite discuté de différents sujets à propos des musiques Turque et Occidentale. J'ai alors constaté que les étudiants de ce conservatoire n'ont aucune connaissance sur la musique Turque en générale. J'ai alors décidé d’effectuer une analyse théorique sur les questions fondamentales de la musique Occidentale etde la musique Turque. Je pense qu'il est important de donner des informations sur la théorie de la musique dans les établissements scolaires occidentaux. Ceci est important pour la communication entre les deux cultures musicales. En rédigeant mon article j'ai utilisé la méthode d'analyse de documents. J'ai aussi utilisé mes expériences personnelles acquises au Conservatoire pour faire un travail de comparaison appliquée. J'ai constaté qu'en termes de gamme, rythme et tonalité il y avait beaucoup de différences mais également des similitudes dans la musique Turque et Occidentale. L'objectif de cet article est de comparer les théories musicales Turque et Occidentale c’est à dire étudier leurs aspects similaires et différents en terme de gamme, rythme et de tonalité.

  16. Polypharmacology directed compound data mining: identification of promiscuous chemotypes with different activity profiles and comparison to approved drugs.

    Science.gov (United States)

    Hu, Ye; Bajorath, Jürgen

    2010-12-27

    Increasing evidence that many pharmaceutically relevant compounds elicit their effects through binding to multiple targets, so-called polypharmacology, is beginning to change conventional drug discovery and design strategies. In light of this paradigm shift, we have mined publicly available compound and bioactivity data for promiscuous chemotypes. For this purpose, a hierarchy of active compounds, atomic property based scaffolds, and unique molecular topologies were generated, and activity annotations were analyzed using this framework. Starting from ∼35 000 compounds active against human targets with at least 1 μM potency, 33 chemotypes with distinct topology were identified that represented molecules active against at least 3 different target families. Network representations were utilized to study scaffold-target family relationships and activity profiles of scaffolds corresponding to promiscuous chemotypes. A subset of promiscuous chemotypes displayed a significant enrichment in drugs over bioactive compounds. A total of 190 drugs were identified that had on average only 2 known target annotations but belonged to the 7 most promiscuous chemotypes that were active against 8-15 target families. These drugs should be attractive candidates for polypharmacological profiling.

  17. Phylogenetic and chemotypic diversity of Periglandula species in eight new morning glory hosts (Convolvulaceae).

    Science.gov (United States)

    Beaulieu, Wesley T; Panaccione, Daniel G; Ryan, Katy L; Kaonongbua, Wittaya; Clay, Keith

    2015-01-01

    Periglandula ipomoeae and P. turbinae (Ascomycota, Clavicipitaceae) are recently described fungi that form symbiotic associations with the morning glories (Convolvulaceae) Ipomoea asarifolia and Turbina corymbosa, respectively. These Periglandula species are vertically transmitted and produce bioactive ergot alkaloids in seeds of infected plants and ephemeral mycelia on the adaxial surface of young leaves. Whether other morning glories that contain ergot alkaloids also are infected by Periglandula fungi is a central question. Here we report on a survey of eight species of Convolvulaceae (Argyreia nervosa, I. amnicola, I. argillicola, I. gracilis, I. hildebrandtii, I. leptophylla, I. muelleri, I. pes-caprae) for ergot alkaloids in seeds and associated clavicipitaceous fungi potentially responsible for their production. All host species contained ergot alkaloids in four distinct chemotypes with concentrations of 15.8-3223.0 μg/g. Each chemotype was a combination of four or five ergot alkaloids out of seven alkaloids detected across all hosts. In addition, each host species exhibited characteristic epiphytic mycelia on adaxial surfaces of young leaves with considerable interspecific differences in mycelial density. We sequenced three loci from fungi infecting each host: the nuclear rDNA internal transcribed spacer region (ITS), introns of the translation factor 1-α gene (tefA) and the dimethylallyl-tryptophan synthase gene (dmaW), which codes for the enzyme that catalyzes the first step in ergot alkaloid biosynthesis. Phylogenetic analyses confirmed that these fungi are in the family Clavicipitaceae and form a monophyletic group with the two described Periglandula species. This study is the first to report Periglandula spp. from Asian, Australian, African and North American species of Convolvulaceae, including host species with a shrub growth form and host species occurring outside of the tropics. This study demonstrates that ergot alkaloids in morning glories

  18. Chemical composition of teas from two cultivated chemotypes of Egletes viscosa ('Macela-da-terra')

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Gizelle Angela B.; Lima, Anne S.; Silveira, Edilberto R. [Ceara Univ., Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica. Curso de Pos-Graduacao em Quimica Organica]. E-mail: edil@ufc.br; Bezerra, Antonio Marcos E. [Ceara Univ., Fortaleza, CE (Brazil). Dept. de Fitotecnia

    2006-01-15

    Phytochemical analysis of flower buds infusion from two cultivated chemotypes of Egletes viscosa Less was accomplished. The new diterpene 12-acetoxy-7-hydroxy-3,13(14)- clerodandien-18,19:15,16-diolide, ternatin, centipedic acid and 12-acetoxy-hawtriwaic lactone were isolated from the chemotype trans-pinocarveyl acetate. Analysis of the chemotype cisisopinocarveyl acetate yielded 12-acetoxy-7-hydroxy-3,13(14)-clerodandien-18,19:15,16- diolide, 12-epi-bacchotricuneatin, ternatin and scopoletin. Structural elucidation of the isolated compounds was established on the basis of spectral data through the use of 1D NMR and several 2D shift correlated NMR pulse sequences and comparison with literature data. (author)

  19. Sesquiterpene lactones in Arnica montana: helenalin and dihydrohelenalin chemotypes in Spain.

    Science.gov (United States)

    Perry, Nigel B; Burgess, Elaine J; Rodríguez Guitián, Manuel A; Romero Franco, Rosa; López Mosquera, Elvira; Smallfield, Bruce M; Joyce, Nigel I; Littlejohn, Roger P

    2009-05-01

    An analytical RPLC method for sesquiterpene lactones in Arnica montana has been extended to include quantitative analyses of dihydrohelenalin esters. LC-ESI-MS-MS distinguished the isomeric helenalin and dihydrohelenalin esters. The dihydrohelenalin esters have lower response factors for UV detection than do helenalin esters, which must be taken into account for quantitative analyses. Analyses of flowers from 16 different wild populations of A. montana in Spain showed differing proportions of helenalin and dihydrohelenalin esters. For the first time a chemotype with high levels of helenalin esters (total helenalins 5.2-10.3 mg/g dry weight) is reported in Spanish A. montana. These samples were from heath lands at high altitude (1330-1460 m), whereas samples from meadows and peat bogs at lower altitudes were the expected chemotype with high levels of dihydrohelenalin esters (total dihydrohelenalins 10.9-18.2 mg/g). The phenolic compounds, both flavonoid glycosides and caffeoylquinic acids, in Spanish A. montana are reported for the first time. The levels of several of these compounds differed significantly between samples from heath lands and samples from peat bogs or meadows, with the heath land samples being most similar to central European A. montana in their phenolic composition.

  20. Selection of new clones of linalool chemotype from genetic recombination in Lippia alba

    Directory of Open Access Journals (Sweden)

    Elcio Rodrigo Rufino

    2012-01-01

    Full Text Available The aromatic and medicinal species Lippia alba is vigorous and rugged native to the South America (Atlantic Rainforest. Because it is an allogamous and self-incompatible species, natural populations have high morphological and chemical variability. This work had as objective to conduct a preliminary screening to identify new promising clones from a novel (recombinant base population of Lippia alba with regard to its agronomic and phytochemical traits, using the linalool oil or chemotype as model. The two superior linalool clones, obtained by collection, were used as controls. Traits evaluated included: dry mass of leaves (DML, oil yield percentage (EOY%, oil production per plant (OP, and linalool percentage (LN%. Forty linalool chemotype clones were evaluated in three experiments, in a random block design with four replicates and four cuttings (clones per plot. Besides means comparisons, multivariate analysis was used in order to aid in the preliminary selection of clones. There were positive correlations from moderate to strong for DML vs. EOY%, OP vs. EOY% and DML vs. OP. Linalool clones superior or similar to both controls were identified for the DML, EOY%, OP, and LN% traits (univariate analyses, aimed at further validating experimentation. Five distinct groups were defined in the cluster analysis (UPGMA, each containing subgroups as well.

  1. The discovery of novel human androgen receptor antagonist chemotypes using a combined pharmacophore screening procedure.

    Science.gov (United States)

    Voet, Arnout; Helsen, Christine; Zhang, Kam Y J; Claessens, Frank

    2013-04-01

    Unraveling the mechanisms involved in castration- and therapy-resistant prostate cancer has led to a renewed interest in androgen receptor (AR)-targeted therapeutics. Anti-androgens that block the activity of the AR therefore remain a valid therapeutic option. However, they must be more effective than, or display a distinct mechanism of action or binding mode from those of bicalutamide and hydroxyflutamide, which are currently in clinical use. For that reason, the second-generation anti-androgen MDV3100 was developed. MDV3100, however, shares its 4-cyano-3-(trifluoromethyl)phenyl group with bicalutamide and hydroxyflutamide required for binding to the AR. In this work, we used a combined strategy to find new antagonist structures distinct from the 4-cyano-3-(trifluoromethyl)phenyl group to avoid cross-resistance for these compounds and to find structures without agonist activity on mutant ARs (AR W741C and AR T877A). We found two novel chemotypes with AR-antagonistic activity (IC(50): 3-6 μM) by virtual screening and confirmed their biological activity in an androgen-responsive reporter assay. The design of our computational approach was validated by the observation of strongly decreased or absence of agonistic activity on the two mutant ARs. Further structural derivatization to optimize the potency of these compounds can render these chemotypes into very promising, alternative AR antagonists for prostate cancer therapy.

  2. La France, l’Europe occidentale et la Palestine, 1799-1917

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    Dominique Trimbur

    2008-06-01

    Full Text Available Organisé par le Centre de recherche français de Jérusalem, le colloque « La France, l’Europe occidentale et la Palestine, 1799-1917 » était la première concrétisation du projet Gallia Palestina. Cette rencontre a prouvé qu’au-delà de points en apparence connus, les champs d’analyses inexplorés restent vastes. Une telle constatation a été rendue possible par les interventions d’un grand nombre de participants, intervenants et public, venus de France, d’Israël ou des territoires palestiniens. U...

  3. Sexualités occidentales, xviiie-xxie siècles

    OpenAIRE

    Diaz, Elvire

    2015-01-01

    Jean-Louis Guereña, professeur émérite de civilisation espagnole à l’université François Rabelais de Tours et auteur récemment de l’ouvrage Les Espagnols et le sexe, xixe-xxe siècles (PUR, 2013), a réuni dans ce gros volume collectif, Sexualités occidentales, xviiie-xxie siècles, vingt-deux contributions pluridisciplinaires et pluriculturelles, qui interrogent le thème comme objet d’histoire à partir des discours, des pratiques et des représentations, et comme grille d’analyse des cultures et...

  4. Diagnose laboratorial dos frutos e folhas de Anacardium occidentale L. (Caju

    Directory of Open Access Journals (Sweden)

    Luzia llza Ferreira Jorge

    1996-01-01

    Full Text Available Anacardium occidentale L. espécie nativa do continente centro e sul-americano, é planta amplamente utilizada, empregada na alimentação humana e animal, bem como na medicina popular. Neste trabalho objetivamos o reconhecimento das principais características diagnósticas das folhas e dos receptáculos carnosos. Detalhes como o tipo de cutícula, paredes celulares, anexos epidérmicos e inclusões celulares são destacados nas descrições e nas figuras. As folhas encerram flavonóides, saponinas,compostos fenólicos e óleo essencial.

  5. Synthesis characterization and catalytic action of hexagonal gold nanoparticles using essential oils extracted from Anacardium occidentale

    Science.gov (United States)

    Sheny, D. S.; Mathew, Joseph; Philip, Daizy

    2012-11-01

    A new phytochemical method for the synthesis of gold nanoparticles is reported. The essential oils extracted from the fresh leaves of Anacardium occidentale are used for the reduction of auric acid to Au nanoparticles (NPs). The formation and morphology of synthesized NPs are investigated with the help of UV-visible, TEM and FTIR spectroscopy. The NPs synthesized at room temperature are mono-dispersed and hexagonal in shape with an average size of 36 nm while those prepared at higher temperature are composed of a mixture of anisotropic particles. The UV-visible absorption spectra of these anisotropic NPs show asymmetry in the longer wavelength side. The quantity of oil is an important criterion modulating the shape of NPs. Possible biochemical mechanism leading to the formation of NPs is studied using FTIR spectroscopy. The potential of synthesized Au NPs as catalyst is explored for the hydrogenation of p-nitro phenol to p-amino phenol at room temperature.

  6. In vitro antifungal activity of four chemotypes of Lippia alba (Verbenaceae essential oils against Alternaria solani (Pleosporeaceae isolates

    Directory of Open Access Journals (Sweden)

    ELISA Z. TOMAZONI

    2016-06-01

    Full Text Available Several volatile natural compounds produced by plant secondary metabolism have been proven to present antimicrobial action, enabling their use in phytopathogen control. They also present low environmental impact when compared to conventional pesticides. Essential oils contain these compounds and can be found in several plant species, such as Lippia alba (Mill. N.E. Brown (Verbenaceae. Essential oils of four chemotypes of L. alba, characterized by their major compounds, namely camphor, citral, linalool and camphor/1,8-cineole, were tested against the phytopathogen Alternaria solani Sorauer (Pleosporaceae, which causes early blight on tomatoes and is responsible for great economic losses regarding production. Essential oils antifungal action was tested in vitro using potato dextrose agar medium with essential oil concentrations at 0.1, 0.5, 1.0, 1.5 and 2.0 µL mL-1. The chemotype that had the best performance was citral, showing significant inhibition compared to the others, starting at the 0.5 µL mL-1 concentration. The essential oil belonging to the linalool chemotype was efficient starting at the 1.5 µL mL-1 concentration. Conversely, the camphor chemotype did not show any action against the phytopathogen. Moreover, the essential oils had no remarkable effect on tomato germination and growth. In conclusion, these essential oils presented fungicidal action against A. solani.

  7. In vitro antifungal activity of four chemotypes of Lippia alba (Verbenaceae) essential oils against Alternaria solani (Pleosporeaceae) isolates.

    Science.gov (United States)

    Tomazoni, Elisa Z; Pansera, Márcia R; Pauletti, Gabriel F; Moura, Sidnei; Ribeiro, Rute T S; Schwambach, Joséli

    2016-05-31

    Several volatile natural compounds produced by plant secondary metabolism have been proven to present antimicrobial action, enabling their use in phytopathogen control. They also present low environmental impact when compared to conventional pesticides. Essential oils contain these compounds and can be found in several plant species, such as Lippia alba (Mill.) N.E. Brown (Verbenaceae). Essential oils of four chemotypes of L. alba, characterized by their major compounds, namely camphor, citral, linalool and camphor/1,8-cineole, were tested against the phytopathogen Alternaria solani Sorauer (Pleosporaceae), which causes early blight on tomatoes and is responsible for great economic losses regarding production. Essential oils antifungal action was tested in vitro using potato dextrose agar medium with essential oil concentrations at 0.1, 0.5, 1.0, 1.5 and 2.0 µL mL-1. The chemotype that had the best performance was citral, showing significant inhibition compared to the others, starting at the 0.5 µL mL-1 concentration. The essential oil belonging to the linalool chemotype was efficient starting at the 1.5 µL mL-1 concentration. Conversely, the camphor chemotype did not show any action against the phytopathogen. Moreover, the essential oils had no remarkable effect on tomato germination and growth. In conclusion, these essential oils presented fungicidal action against A. solani.

  8. O uso da casca da castanha do caju, Anacardium occidentale, como moluscicida alternativo The use of cashew nut shell of caju (Anacardium occidentale as alternative molluscicide

    Directory of Open Access Journals (Sweden)

    Cecília Pereira de Souza

    1992-10-01

    Full Text Available Bioensaios usando extratos hexânicos de cascas da castanha do caju, Anacardium occidentale, coletadas no Ceará em 1972 (amostra 1 e em 1987 (amostra 2 foram feitos sobre moluscos adultos e desovas de Biomphalaria glabrata, B. tenagophila e B. straminea, no laboratório e no campo. As cascas, 18,5 g, sem triturar amostra 1, também foram testadas sobre moluscos adultos e desovas das três espécies. A toxidez do extrato foi testada ainda sobre peixes (Poecilia reticulata e girinos. As concentrações letais CL90, amostra 1, foram de 2,0 a 2,2 ppm para os moluscos das três espécies. Para B. glabrata adultos, recém-eclodidos e desovas as CL90, amostra 2, foram de 2,0, 0,5 e 30,0 ppm respectivamente. As cascas causaram mortalidade de 40 a 80% dos moluscos e de 22 a 35% dos embriões, ocasionando redução de 40 a 55% na oviposição das três espécies. O extrato hexânico, amostra 2, foi inócuo para girinos e peixes até 2 ppm. No campo, em poços com água parada, tratados com 20 ppm do extrato, amostra 1, ocorreu 97,1% de mortalidade de B. straminea e 100% de B. glabrata e B. tenagophila. Com a niclosamida a 3 ppm ocorreu 100% de mortalidade das três espécies.Bioassays using hexanolic extracts of cashew nut shells, of Anacardium occidentale, collected in Ceará in 1972 (Sample 1 and 1987 (Sample 2 were undertaken with adult snails and egg masses of Biomphalaria glabrata, B. tenagophila and B. straminea both in the laboratory and in the field. Non extracted shells, 18.5 g, sample 1, were also tested with adult snails and egg masses of the three species. The toxicity of extract was tested with fish (Poecilia reticulata and tadpoles. The lethal concentration, CL90, of sample 1 was from 2.0 to 2.2 ppm for adult snails of the three species. With sample 2, the CL90 was 2.0, 0.5 and 30.0 ppm for B. glabrata adults, newly hatched snails and egg mass respectively. Non extracted shells caused 40 - 80% mortality of adult snails, 22 - 35% mortality

  9. Efficacy of Withania somnifera chemotypes NMITLI - 101R, 118R and Withaferin A against experimental visceral leishmaniasis.

    Science.gov (United States)

    Tripathi, C D P; Gupta, R; Kushawaha, P K; Mandal, C; Misra Bhattacharya, S; Dube, A

    2014-06-01

    The immunoprophylactic and therapeutic potentials of root extracts of Withania somnifera chemotypes (NMITLI-118, NMITLI-101) and pure withanolide-withaferin A was investigated against Leishmania donovani infection in hamsters. The naive animals, fed orally with immunostimulatory doses of chemotypes 101R, 118R (10 and 3 mg/kg) and withaferin A (9 and 3 mg/kg) for five consecutive days and challenged with Leishmania parasites on day 6, were euthanized on days 30 and 45 p.c. for the assessment of parasite clearance, real-time analysis of mRNAs of Th1/Th2 cytokines (IFN-γ, IL-12, TNF-α, iNOS/IL-4, IL-10 and TGF-β), NO production, reactive oxygen species (ROS) generation, lymphocyte transformation test and antibody responses. By day 45 p.c., there was a significant increase in the mRNA expression of iNOS, IFN-γ, IL-12 and TNF-α but decrease in IL-4, IL-10 and TGF-β, an enhanced Leishmania-specific LTT response as well as ROS, NO and antileishmanial IgG2 levels in 101R-treated hamsters followed by 118R- and withaferin A-treated ones, respectively. When these chemotypes were given to L. donovani-infected hamsters at different doses, there was moderate therapeutic efficacy of chemotype 101R (~50%) at 30 mg/kg × 5 followed by the other two. The results established that the 101R is the most potential chemotype and can be evaluated for combination therapy along with available antileishmanials.

  10. Influence of gamma radiation on the antioxidant action extracts from leaves of gross Anacardium occidentale Linn; Influencia da radiacao gama na acao antioxidante de extratos brutos de folhas de Anacardium occidentale Linn.

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Gustavo Henrique Farias dos; Silva, Edvane Borges da; Amaral, Ademir de Jesus [Universidade Federal de Pernambuco (GERAR/DEN/UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Grupo de Estudos em Radioprotecao e Radioecologia; Lima, Claudia Sampaio de Andrade [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Departamento de Biofisica e Radiobiologia

    2014-07-01

    Anacardium occidentale Linn. is popularly known as cashew plant, found in Northeastern Brazil, is of great scientific interest because it contains high levels of bioactive compounds, such as polyphenols, flavonoids and tannins, which characterize their applications as natural antioxidants, which can contribute to protect against oxidative processes in the human body. This study aimed to evaluate the effects of {sup 60}Co gamma irradiation in the antioxidant action of the extracts of leaves of A. occidentale. The extracts of A. occidentale were extracted with 70% ethanol, evaporated under reduced pressure and divided into samples control and irradiation at 10 kGy. Subsequently analyzing the kidnapping of radical DPPH (concentrations of 6.25; 12.5; 25; 50; 100 and 200 ppm) and reducing power (concentrations of 25; 50; 100; 200; 400; 600; 800 and 1000 ppm). The results showed increased antioxidant actions dependent on the concentration and dose of 10 kGy in both assays. However, the kidnapping of DPPH activity showed variations from 50% to 92% for control, and 75% and 100% for irradiated. It is observed that the irradiated samples showed maximum activity (100%) in a 4-fold lower concentration (50 ppm) than the control samples. Featuring EC{sub 50} of standards BHT (235.8%) and vitamin C (63.5%) was very low compared with control extracts (8.1%) and irradiated (0.48). However the results obtained in this study indicate that extracts of leaves of A. occidentale irradiated at a dose of 10 kGy exhibit strong antioxidant activity against DPPH and power reducer, this paves its use as a natural source of antioxidants.

  11. Identification and characterization of a new chemotype of noncovalent SENP inhibitors.

    Science.gov (United States)

    Madu, Ikenna G; Namanja, Andrew T; Su, Yang; Wong, Steven; Li, Yi-Jia; Chen, Yuan

    2013-07-19

    Enzymes called SENPs catalyze both the maturation of small ubiquitin-like modifier (SUMO) precursors and removal of SUMO modifications, which regulate essential cellular functions such as cell cycle progression, DNA damage response, and intracellular trafficking. Some members, such as SENP1, are potential targets for developing cancer therapeutics. We searched for small molecule inhibitors of SENPs using in silico screening in conjunction with biochemical assays and identified a new chemotype of small molecule inhibitors that noncovalently inhibit SENPs. The inhibitors confer the noncompetitive inhibitory mechanism, as shown by nuclear magnetic resonance (NMR) and quantitative enzyme kinetic analysis. The NMR data also provided evidence for substrate-assisted inhibitor binding, which indicates the need for caution in using artificial substrates for compound screening, as the inhibitory effects could be significantly different from using the physiological substrates. This finding also suggests the possibility of designing inhibitors for this class of enzymes that are tuned for substrate-specificity.

  12. Discovery of Potent and Highly Selective A2B Adenosine Receptor Antagonist Chemotypes.

    Science.gov (United States)

    El Maatougui, Abdelaziz; Azuaje, Jhonny; González-Gómez, Manuel; Miguez, Gabriel; Crespo, Abel; Carbajales, Carlos; Escalante, Luz; García-Mera, Xerardo; Gutiérrez-de-Terán, Hugo; Sotelo, Eddy

    2016-03-10

    Three novel families of A2B adenosine receptor antagonists were identified in the context of the structural exploration of the 3,4-dihydropyrimidin-2(1H)-one chemotype. The most appealing series contain imidazole, 1,2,4-triazole, or benzimidazole rings fused to the 2,3-positions of the parent diazinone core. The optimization process enabled identification of a highly potent (3.49 nM) A2B ligand that exhibits complete selectivity toward A1, A2A, and A3 receptors. The results of functional cAMP experiments confirmed the antagonistic behavior of representative ligands. The main SAR trends identified within the series were substantiated by a molecular modeling study based on a receptor-driven docking model constructed on the basis of the crystal structure of the human A2A receptor.

  13. Exploring the 3-piperidin-4-yl-1H-indole scaffold as a novel antimalarial chemotype.

    Science.gov (United States)

    Santos, Sofia A; Lukens, Amanda K; Coelho, Lis; Nogueira, Fátima; Wirth, Dyann F; Mazitschek, Ralph; Moreira, Rui; Paulo, Alexandra

    2015-09-18

    A series of 3-piperidin-4-yl-1H-indoles with building block diversity was synthesized based on a hit derived from an HTS whole-cell screen against Plasmodium falciparum. Thirty-eight compounds were obtained following a three-step synthetic approach and evaluated for anti-parasitic activity. The SAR shows that 3-piperidin-4-yl-1H-indole is intolerant to most N-piperidinyl modifications. Nevertheless, we were able to identify a new compound (10d) with lead-like properties (MW = 305; cLogP = 2.42), showing antimalarial activity against drug-resistant and sensitive strains (EC50 values ∼ 3 μM), selectivity for malaria parasite and no cross-resistance with chloroquine, thus representing a potential new chemotype for further optimization towards novel and affordable antimalarial drugs.

  14. Some phytochemical studies or the genera Aconitum L., Delphinium L. and Consolida (DC. S.F. Gray

    Directory of Open Access Journals (Sweden)

    de la Fuente, Gabriel

    1990-12-01

    Full Text Available The diterpenoid alkaloids from Aconitum napellus L. subsp. castellanum Molera & Blanché, A. Burnatii Gayer, A. Compactum Reichenb., Delphinium gracile DC., D. pentagynum Lam., D. verdunense Balbis (= D. cardiopetalum DC., D. Requienii DC.. Consolida orientalis (Gay Schröd, subsp. orientalis i C. pubescens (OC. Soó. distributed in the Iberian Peninsula, and A . napellus L. s. Str. (from England, D. montanum DC. (from France, D. nanum Dc. subsp. elongatum (Boiss Blanché & al. (= D. peregrinum L. var. elongatum Boiss. (from Morocco i D. pictum Willd. (from Mallorca are reviewed. The alkaloid content, structural types. and oxygenation patterns are discussed with respect to related species.

    [ca] Es revisen els alcaloides diterpènics obtinguts d'Aconitum napellus L. subsp. castellanum Molera & Blanché, A. Burnatii Gayer, A. Compactum Reichenb., Delphinium gracile DC., D. pentagynum Lam., D. verdunense Balbis (= D. cardiopetalum DC., D. Requienii DC.. Consolida orientalis (Gay Schröd, subsp. orientalis and C. pubescens (OC. Soó. distribuïts a la Península Ibèrica, així com d'A . napellus L. s. Str. (d'Anglaterra, D. montanum DC. (de França, D. nanum Dc. subsp. elongatum (Boiss Blanché & al. (= D. peregrinum L. var. elongatum Boiss. (del Marroc i D. pictum Willd. (de Mallorca. El contingut en alcaloides, llurs tipus estructurals i models d'oxigenació són discutits en relació a d'alt res espècies afins.

  15. Genotypic and chemotypic diversity of Neotyphodium endophytes in tall fescue from Greece.

    Science.gov (United States)

    Takach, Johanna E; Mittal, Shipra; Swoboda, Ginger A; Bright, Sherrita K; Trammell, Michael A; Hopkins, Andrew A; Young, Carolyn A

    2012-08-01

    Epichloid endophytes provide protection from a variety of biotic and abiotic stresses for cool-season grasses, including tall fescue. A collection of 85 tall fescue lines from 15 locations in Greece, including both Continental and Mediterranean germplasm, was screened for the presence of native endophytes. A total of 37 endophyte-infected lines from 10 locations were identified, and the endophytes were classified into five distinct groups (G1 to G5) based on physical characteristics such as colony morphology, growth rate, and conidial morphology. These classifications were supported by phylogenetic analyses of housekeeping genes tefA and tubB, and the endophytes were further categorized as Neotyphodium coenophialum isolates (G1, G4, and G5) or Neotyphodium sp. FaTG-2 (Festuca arundinacea taxonomic group 2 isolates (G2 and G3). Analyses of the tall fescue matK chloroplast genes indicated a population-wide, host-specific association between N. coenophialum and Continental tall fescue and between FaTG-2 and Mediterranean tall fescue that was also reflected by differences in colonization of host tillers by the native endophytes. Genotypic analyses of alkaloid gene loci combined with chemotypic (chemical phenotype) profiles provided insight into the genetic basis of chemotype diversity. Variation in alkaloid gene content, specifically the presence and absence of genes, and copy number of gene clusters explained the alkaloid diversity observed in the endophyte-infected tall fescue, with one exception. The results from this study provide insight into endophyte germplasm diversity present in living tall fescue populations.

  16. Laboratory and field measurements of enantiomeric monoterpene emissions as a function of chemotype, light and temperature

    Science.gov (United States)

    Song, W.; Staudt, M.; Bourgeois, I.; Williams, J.

    2014-03-01

    Plants emit significant amounts of monoterpenes into the earth's atmosphere, where they react rapidly to form a multitude of gas phase species and particles. Many monoterpenes exist in mirror-image forms or enantiomers. In this study the enantiomeric monoterpene profile for several representative plants (Quercus ilex L., Rosmarinus officinalis L., and Pinus halepensis Mill.) was investigated as a function of chemotype, light and temperature both in the laboratory and in the field. Analysis of enantiomeric monoterpenes from 19 Quercus ilex individuals from Southern France and Spain revealed four regiospecific chemotypes (genetically fixed emission patterns). In agreement with previous work, only Quercus ilex emissions increased strongly with light. However, for all three plant species no consistent enantiomeric variation was observed as a function of light, and the enantiomeric ratio of α-pinene was found to vary by less than 20% from 100 and 1000 μmol m-2 s-1 PAR (photosynthetically active radiation). The rate of monoterpene emission increased with temperature from all three plant species, but little variation in the enantiomeric distribution of α-pinene was observed with temperature. There was more enantiomeric variability between individuals of the same species than could be induced by either light or temperature. Field measurements of α-pinene enantiomer mixing ratios in the air, taken at a Quercus ilex forest in Southern France, and several other previously reported field enantiomeric ratio diel cycle profiles are compared. All show smoothly varying diel cycles (some positive and some negative) even over changing wind directions. This is surprising in comparison with variations of enantiomeric emission patterns shown by individuals of the same species.

  17. Assessment of Phenolic Compounds and Anti-Inflammatory Activity of Ethyl Acetate Phase of Anacardium occidentale L. Bark

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    Marina Suênia de Araújo Vilar

    2016-08-01

    Full Text Available The bark of A. occidentale L. is rich in tannins. Studies have described various biological activities of the plant, including antimicrobial, antioxidant, antiulcerogenic and antiinflammatory actions. The objective of this study was to assess the activity of the ethyl acetate phase (EtOAc of A. occidentale on acute inflammation and to identify and quantify its phenolic compounds by HPLC. The method was validated and shown to be linear, precise and accurate for catechin, epicatechin, epigallocatechin and gallic acid. Swiss albino mice (Mus musculus were treated with saline, Carrageenan (2.5%, Indomethacin (10 mg/kg, Bradykinin (6 nmol and Prostaglandine E2 (5 µg at different concentrations of EtOAc - A. occidentale (12.5; 25; 50; and 100 mg/kg/weight p.o. for the paw edema test. Challenge was performed with carrageenan (500 µg/mL i.p. for the doses 50 and 100 mg/kg of EtOAc. Levels of cytokines IL-1, TNF-α, IL-6 and IL-10 were also measured. All EtOAc - A. occidentale concentrations reduced the edema. At 50 and 100 mg/kg, an anti-inflammatory response of the EtOAc was observed. Carrageenan stimulus produced a neutrophil count of 28.6% while 50 and 100 mg/kg of the phase reduced this to 14.5% and 9.1%, respectively. The EtOAc extract reduced levels of IL-1 and TNF-α. These results suggest that the EtOAc plays a modulatory role in the inflammatory response. The chromatographic method can be used for the analysis of the phenolic compounds of the EtOAc phase.

  18. Effect of Biofield Energy Treatment on Chlorophyll Content, Pathological Study, and Molecular Analysis of Cashew Plant (Anacardium occidentale L.)

    OpenAIRE

    Trivedi, Mahendra Kumar

    2015-01-01

    In the world scenario, India occupies a premier position contributing to about 43 per cent production of the cashew nut (Anacardium occidentale L.) along with export and processing. The aim is to study the impact of biofield energy treatment on selected farms for cashew farming. The control and biofield treated farms were divided as control and treated farms, and Mr. Trivedi provided the biofield energy treatment to the treated farms. Further, the plants and fruits were analyzed for overall g...

  19. Contributo di dati di gravità nella valutazione del vulcanismo CAMP in Africa Nord-Occidentale

    OpenAIRE

    Fabbri, Julius

    2015-01-01

    La domanda alla base di questa ricerca è stata se il metodo della gravimetria satellitare possa essere utilizzato per seguire le unità geologiche anche in luoghi difficilmente accessibili. L’obiettivo di questa ricerca è di verificare se le missioni satellitari di nuova generazione permettano di identificare la più grande delle province ignee della Terra (Bertrand et al., 2013), nota come CAMP (Central Atlantic Magmatic Province) in Africa nord-occidentale. Oltre alle motivazioni scientifiche...

  20. Anacardium occidentale Linn. (Anacardiaceae) stem bark extract induces hypotensive and cardio-inhibitory effects in experimental animal models.

    Science.gov (United States)

    Tchikaya, Francis Olivier; Bantsielé, Guy Bernard; Kouakou-Siransy, Gisèle; Datté, Jacques Yao; Yapo, Paul Angoue; Zirihi, Noel Guedé; Offoumou, Michel Atté

    2011-01-01

    Anacardium occidentale Linn. (Anacardiaceae) is a plant largely used in Africa for the treatment of different diseases. In Côte d'Ivoire it's commonly used for the treatment of hypertension. The present study was carried out in order to assess the effects of Anacardium occidentale extract (ANOE) on cardiovascular parameters in animal models. A mercury manometer kymograph of Ludwig was used to measure the blood pressure of normotensive rabbits in control conditions (normal physiological solution) and under the influence of ANOE. The contractile activity of an isolated rat heart was also measured in control conditions and under the influence of ANOE in different physiological media using a modified Langendhorff (1895) apparatus. The aqueous Anacardium occidentale (ANOE) bark extract applied intravenously in different doses (12, 40, 90, and 167 mg/kg b.w.), produced a significant dose-dependent decrease in blood pressure of previously normotensive rabbits (up to 89% vs control). Atropine (1 mg/ml) pre-treatment failed to reverse the hypotensive effects elicited by the extract. ANOE applied to isolated rat heart preparations in different concentrations (0.01, 0.1, 1.0, and 10 µg/ml) induced negative inotropic and chronotropic effects. Atropine pre-treatment of heart preparations (0.1 µg/ml) failed to reverse the negative effects induced by ANOE. The extract's action on heart contractile activity studied in modified culture media further confirmed its cardio-inhibitory effects. ANOE induced strong hypotensive and cardio-inhibitory effects in animal models.

  1. Membrane stability of sickle erythrocytes incubated in extracts of three medicinal plants: Anacardium occidentale, Psidium guajava, and Terminalia catappa

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    Paul Chidoka Chikezie

    2011-01-01

    Full Text Available Background: Many reports showed that medicinal plant extracts cause alterations on the shape and physiology of erythrocytes. Objective: The present study seeks to ascertain the osmotic stability of sickle erythrocytes incubated in aqueous extracts of Anacardium occidentale, Psidium guajava, and Terminalia catappa. Materials and Methods: The fraction of erythrocytes lysed when suspended in saline solution of varying concentrations was investigated by spectrophotometric method. The percentage hemolysis of erythrocytes in the control and test samples showed a sigmoidal relationship with increasing concentrations of saline solution. Membrane stability was ascertained as mean corpuscular fragility (MCF index of erythrocytes incubated in 400 and 800 mg/dL aqueous concentrations of the three plant extracts. Results: The two experimental concentrations of P. guajava and T. catappa protected the erythrocytes against osmotic stress, as evidenced by decreases in the values of MCF compared with the control sample (P < 0.05. However, 800 mg/dL of A. occidentale promoted significant (P < 0.05 distabilization of sickle erythrocytes. Conclusion: Whereas the two experimental concentrations of aqueous extracts of P. guajava and T. catappa stabilized erythrocyte membrane, higher concentration (800 mg/dL of A. occidentale exhibited no membrane protective effect.

  2. Application of terrestrial photogrammetry for the mass balance calculation on Montasio Occidentale Glacier (Julian Alps, Italy)

    Science.gov (United States)

    Piermattei, Livia; Carturan, Luca; Calligaro, Simone; Blasone, Giacomo; Guarnieri, Alberto; Tarolli, Paolo; Dalla Fontana, Giancarlo; Vettore, Antonio

    2014-05-01

    Digital elevation models (DEMs) of glaciated terrain are commonly used to measure changes in geometry and hence infer the mass balance of glaciers. Different tools and methods exist to obtain information about the 3D geometry of terrain. Recent improvements on the quality and performance of digital cameras for close-range photogrammetry, and the development of automatic digital photogrammetric processing makes the 'structure from motion' photogrammetric technique (SfM) competitive for high quality 3D models production, compared to efficient but also expensive and logistically-demanding survey technologies such as airborn and terrestrial laser scanner (TLS). The purpose of this work is to test the SfM approach, using a consumer-grade SLR camera and the low-cost computer vision-based software package Agisoft Photoscan (Agisoft LLC), to monitor the mass balance of Montasio Occidentale glacier, a 0.07km2, low-altitude, debris-covered glacier located in the Eastern Italian Alps. The quality of the 3D models produced by the SfM process has been assessed by comparison with digital terrain models obtained through TLS surveys carried out at the same dates. TLS technique has indeed proved to be very effective in determining the volume change of this glacier in the last years. Our results shows that the photogrammetric approach can produce point cloud densities comparable to those derived from TLS measurements. Furthermore, the horizontal and vertical accuracies are also of the same order of magnitude as for TLS (centimetric to decimetric). The effect of different landscape characteristics (e.g. distance from the camera or terrain gradient) and of different substrata (rock, debris, ice, snow and firn) was also evaluated in terms of SfM reconstruction's accuracy vs. TLS. Given the good results obtained on the Montasio Occidentale glacier, it can be concluded that the terrestrial photogrammetry, with the advantageous features of portability, ease of use and above all low costs

  3. Efecto nutracéutico del Anacardium occidentale en dietas de pollitas ponedoras de reemplazo

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    Yordan Martínez A.

    2012-12-01

    Full Text Available Objetivo. Evaluar el efecto nutracéutico del polvo de hojas y retoños de Anacardium occidentale (AO en dietas de pollitas ponedoras de remplazo. Materiales y métodos. Se utilizaron 240 pollitas White Leghorn (L-33 de un día de edad, que se ubicaron durante 35 días, según diseño completamente aleatorizado, con niveles de adición de 0, 0.5, 1.5 y 2.5% de polvo de hojas y retoños de Anacardium occidentale en las dietas. Se determinaron en las pollitas, los indicadores productivos, peso absoluto y relativo de los órganos inmunes, vísceras, accesorios e intestinos, la hipersensibilidad intestinal y la glucosa sérica. Resultados. El peso vivo final, consumo de alimento, peso del timo, bolsa de Fabricio y colon + recto en las aves con el tracto gastrointestinal vacío y lleno, fue favorable con la adición de 0.5% de polvo AO, con diferencias significativas (p≤0.05. El consumo acumulado, el consumo de polvo AO y taninos se incrementaron en las aves con la adición de 1.5 y 2.5% de polvo AO con respecto al control; no obstante los indicadores productivos para estos animales se deprimieron. La adición del polvo de AO, no deterioró el peso relativo de las vísceras (corazón, hígado y riñón en las aves, además redujo la hipersensibilidad intestinal y la glucosa sérica. Conclusiones. La adición de 0.5% de polvo de hojas y retoños de AO como nutracéutico en las dietas de pollitas ponedoras de remplazo, mejoró los indicadores productivos y el peso de los órganos inmunes; además, la adición del polvo AO en las dietas disminuyó la hipersensibilidad intestinal y la glucosa sérica.

  4. Estimates of genetic correlations and correlated responses to selection in cashew (Anacardium occidentale L.

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    Diógenes Manoel Pedroza de Azevedo

    1998-09-01

    Full Text Available The present study estimates variances and genetic and phenotypic correlations for five traits in 27 progenies of cashew trees (Anacardium occidentale L.. Data were obtained from a trial conducted in 1992 at Pacajus, Ceará, experimental station of Embrapa Agroindústria Tropical. The characters studied were plant height (PH, North-South and East-West canopy spreads (NSS, EWS, and primary and secondary branch numbers (PBN, SBN. All genetic and phenotypic correlations presented positive and significant values. Selection to increase or decrease the average of any one of the five characteristics of cashew plants in the progenies studied affected the average of the others. The 16-month-old canopy spread can be predicted from NSS or EWS since correlations between them were high. Correlations between PH and SBN were low, indicating that there is a good possibility of obtaining smaller plants without causing drastic reductions in SBN. PH and SBN showed, respectively, the lowest and highest genetic variance estimates relative to the corresponding population means.Neste trabalho são estimadas variâncias, correlações genéticas e fenotípicas e respostas correlacionadas, envolvendo cinco caracteres em 27 progênies de cajueiro (Anacardium occidentale L.. Os dados foram obtidos em Pacajus-CE, num ensaio conduzido no Campo Experimental da Embrapa Agroindústria Tropical, em l992. Os caracteres estudados foram altura de planta (PH, envergaduras norte-sul (NSS e leste-oeste (EWS e número de ramos primários (PBN e secundários (SBN. Todas as correlacões genéticas e fenotípicas obtidas foram positivas e significativas. A seleção para aumentar ou reduzir a média de qualquer um dos cinco caracteres estudados nas progênies de cajueiro afetou indiretamente a média dos outros quatro caracteres. A envergadura da copa aos 16 meses pode ser representada por NSS ou EWS, tendo em vista que a correlação entre elas foi elevada. As correlações envolvendo PH

  5. Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

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    Tomasz Kulik

    2016-11-01

    Full Text Available Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.

  6. Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

    Science.gov (United States)

    Kulik, Tomasz; Buśko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D.; Perkowski, Juliusz; Stenglein, Sebastian

    2016-01-01

    Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium. PMID:27845742

  7. Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina.

    Science.gov (United States)

    Kulik, Tomasz; Buśko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D; Perkowski, Juliusz; Stenglein, Sebastian

    2016-11-12

    Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.

  8. A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS

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    E. V. Voloshina

    2009-01-01

    Full Text Available Abstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bacterial lipopolysaccharides from S. typhimurium (S-chemotype form, S. typhimurium SL1181 (R-chemotype, Re-mutant, E. coli O55:B5 (S-chemotype, and E. coli JM103 (R-chemotype, Re-mutant were used in this study. A decreased molar ratio for lipid A-KDO in S-form of LPS from E. coli is accompanied by diminished TNFα and IL-6 expression. By the contrast, for S-form of LPS from Salmonella, a decrease in lipid A-KDO molar ratio did cause a sufficient enhancement of TNFα expression. A contribution of lipid A structure into biological activity of LPS is more significant for Re-chemotype than for S-chemotype, independently on bacterial species.

  9. Insect fauna associated with Anacardium occidentale (Sapindales: Anacardiaceae) in Benin, West Africa.

    Science.gov (United States)

    Agboton, C; Onzo, A; Ouessou, F I; Goergen, G; Vidal, S; Tamò, M

    2014-01-01

    Cashew, Anacardium occidentale L. (Sapindales: Anacardiaceae), is an important cash crop in Benin. However, its production is threatened by several biotic factors, especially insects. In Benin, very few studies have focused on insects and just listed species commonly found on cashew worldwide. The present investigation fills this gap by presenting an exhaustive inventory of insect species associated with this crop in the country. The survey was carried out from September 2009 to August 2010 in 22 cashew orchards (5 young and 17 mature) distributed over three major agroecological zones where cashew is most produced in the country. Insects were collected using chemical knock-down technique and visual observation followed by capture with sweep net. In addition, infested plant organs were sampled and incubated to collect emerging insects. In total, 262 insect species were recorded and identified. Among them, the wood borer Apate terebrans Pallas, the leafminer Eteoryctis gemoniella Stainton, and the mirid bugs Helopeltis schoutedeni Reuter., and Helopeltis anacardii Miller., appeared as the most important insect species attacking cashew in Benin. Beneficial insects encountered included some predators, parasitoids, and pollinators. Few vertebrate predators were also recorded on the trees. Differences in agroecological conditions or in field cleanliness did not affect the number of insect species encountered in the cashew orchards. The results of this study represent an important baseline data for the design and implementation of strategies for cashew protection in Benin.

  10. Antiulcerogenic effect and acute toxicity of a hydroethanolic extract from the cashew (Anacardium occidentale L.) leaves.

    Science.gov (United States)

    Konan, Nzi André; Bacchi, Elfriede Marianne

    2007-06-13

    The antiulcerogenic effect of a hydroethanolic extract of Anacardium occidentale L. leaves was investigated. The extract inhibited gastric lesions induced by HCl/ethanol in female rats. A dose-response effect study showed that the ED50 was 150 mg/kgb.w. Extract doses higher than 100 mg/kgb.w. were more effective than 30 mg/kg of lansoprazol in inhibiting gastric lesions. A methanolic fraction (257.12 mg/kg) which reduced gastric lesion at 88.20% is likely to contain the active principle of the antiulcer effect. No signs of acute toxicity were observed when mice were treated with extract dose up to 2000 mg/kgb.w. A chemical analysis of the extract allowed the identification of phenolic compounds as the major components. Glycosylated quercetin, amentoflavone derivate and a tetramer of proanthocyanidin were identified by liquid chromatography coupled to mass spectrometry. The level of total phenolics in the extract was evaluated at 35.5% and flavonoid content was 2.58%.

  11. Enzyme-Assisted Aqueous Extraction Of Cashew Nut (Anacardium occidentale L. Oil

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    Phuong Huynh Nhu Nguyen

    2016-03-01

    Full Text Available Enzyme-assisted aqueous extraction method was applied to extract oil from cashew nut (Anacardium occidentale L.. The commercial enzyme (Viscozyme cassava C was tested for effectiveness in releasing oil during the aqueous extraction. The effect of several parameters such as material/water ratio, enzyme concentration and duration for enzyme incubation on the oil yield was investigated. The conditions for maximum oil release were found with the material/water ratio of 1:9, enzyme concentration of 1% (v/w E/S, and in 3 h of enzyme incubation at 50oC with constant shaking. The maximum oil yield obtained at those conditions (38.88 % raw material was significantly (p <0.05 higher than that of the control (without enzyme (35.92 %, and it represented 86.28 % recovery of the total oil in seed. No hexane and other organic solvents were needed for this process. The cashew nut oil by enzyme-assisted aqueous extraction was relatively stable. Both peroxide value and free fatty acid value were lower than those in the oil obtained by Soxhlet method. Total un-saturated fatty acid in the cashew nut oil was about 84.43 %, in which the most abundant was oleic acid (65.0 %, followed by linoleic acid (18.53%. Cashew nut oil is a good dietary source of un-saturated fatty acids.

  12. 3-H-[1,2]Dithiole as a New Anti-Trypanosoma cruzi Chemotype: Biological and Mechanism of Action Studies

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    Marcos Couto

    2015-08-01

    Full Text Available The current pharmacological Chagas disease treatments, using Nifurtimox or Benznidazole, show limited therapeutic results and are associated with potential side effects, like mutagenicity. Using random screening we have identified new chemotypes that were able to inhibit relevant targets of the Trypanosoma cruzi. We found 3H-[1,2]dithioles with the ability to inhibit Trypanosoma cruzi triosephosphate isomerase (TcTIM. Herein, we studied the structural modifications of this chemotype to analyze the influence of volume, lipophilicity and electronic properties in the anti-T. cruzi activity. Their selectivity to parasites vs. mammalian cells was also examined. To get insights into a possible mechanism of action, the inhibition of the enzymatic activity of TcTIM and cruzipain, using the isolated enzymes, and the inhibition of membrane sterol biosynthesis and excreted metabolites, using the whole parasite, were achieved. We found that this structural framework is interesting for the generation of innovative drugs for the treatment of Chagas disease.

  13. Composition and Bioactivities of an (E)-β-Farnesene Chemotype of Chamomile (Matricaria chamomilla) Essential Oil from Nepal.

    Science.gov (United States)

    Satyal, Prabodh; Shrestha, Samon; Setzer, William N

    2015-08-01

    The essential oil of Matricaria chamomilla, collected from Nepal, was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components in Nepalese chamomile oil were (E)-β-famesene (42.2%), α-bisabolol oxide A (22.3%), (E,E)-α-famesene (8.3%), cis-bicycloether (5.0%), α-bisabolol oxide B (4.5%), and α-bisabolone oxide A (4.0%). A cluster analysis based on the chemical compositions of 48 samples of chamomile oil reported in the literature has revealed seven chemotypes, and the oil from Nepal represents the (E)-β-farnesene chemotype. The chamomile oil was screened for antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger, and toxicity toward MCF-7 breast tumor cells, Artemia salina, Chaoborus plumicornis, Caenorhabditis elegans, and Drosophila melanogaster.

  14. 3-H-[1,2]Dithiole as a New Anti-Trypanosoma cruzi Chemotype: Biological and Mechanism of Action Studies.

    Science.gov (United States)

    Couto, Marcos; Sánchez, Carina; Dávila, Belén; Machín, Valentina; Varela, Javier; Álvarez, Guzmán; Cabrera, Mauricio; Celano, Laura; Aguirre-López, Beatriz; Cabrera, Nallely; de Gómez-Puyou, Marieta Tuena; Gómez-Puyou, Armando; Pérez-Montfort, Ruy; Cerecetto, Hugo; González, Mercedes

    2015-08-12

    The current pharmacological Chagas disease treatments, using Nifurtimox or Benznidazole, show limited therapeutic results and are associated with potential side effects, like mutagenicity. Using random screening we have identified new chemotypes that were able to inhibit relevant targets of the Trypanosoma cruzi. We found 3H-[1,2]dithioles with the ability to inhibit Trypanosoma cruzi triosephosphate isomerase (TcTIM). Herein, we studied the structural modifications of this chemotype to analyze the influence of volume, lipophilicity and electronic properties in the anti-T. cruzi activity. Their selectivity to parasites vs. mammalian cells was also examined. To get insights into a possible mechanism of action, the inhibition of the enzymatic activity of TcTIM and cruzipain, using the isolated enzymes, and the inhibition of membrane sterol biosynthesis and excreted metabolites, using the whole parasite, were achieved. We found that this structural framework is interesting for the generation of innovative drugs for the treatment of Chagas disease.

  15. Intraspecific variation in essential oil composition of the medicinal plant Lippia integrifolia (Verbenaceae). Evidence for five chemotypes.

    Science.gov (United States)

    Marcial, Guillermo; de Lampasona, Marina P; Vega, Marta I; Lizarraga, Emilio; Viturro, Carmen I; Slanis, Alberto; Juárez, Miguel A; Elechosa, Miguel A; Catalán, César A N

    2016-02-01

    The aerial parts of Lippia integrifolia (incayuyo) are widely used in northwestern and central Argentina for their medicinal and aromatic properties. The essential oil composition of thirty-one wild populations of L. integrifolia covering most of its natural range was analyzed by GC and GC-MS. A total of one hundred and fifty two terpenoids were identified in the essential oils. Sesquiterpenoids were the dominant components in all but one of the collections analyzed, the only exception being a sample collected in San Juan province where monoterpenoids amounted to 51%. Five clearly defined chemotypes were observed. One possessed an exquisite and delicate sweet aroma with trans-davanone as dominant component (usually above 80%). Another with an exotic floral odour was rich in oxygenated sesquiterpenoids based on the rare lippifoliane and africanane skeletons. The trans-davanone chemotype is the first report of an essential oil containing that sesquiterpene ketone as the main constituent. The absolute configuration of trans-davanone from L. integrifolia was established as 6S, 7S, 10S, the enantiomer of trans-davanone from 'davana oil' (Artemisia pallens). Wild plants belonging to trans-davanone and lippifolienone chemotypes were propagated and cultivated in the same parcel of land in Santa Maria, Catamarca. The essential oil compositions of the cultivated plants were essentially identical to the original plants in the wild, indicating that the essential oil composition is largely under genetic control. Specimens collected near the Bolivian border that initially were identified as L. boliviana Rusby yielded an essential oil practically identical to the trans-davanone chemotype of L. integrifolia supporting the recent view that L. integrifolia (Gris.) Hieron. and L. boliviana Rusby are synonymous.

  16. Phytochemical Analysis and Antimicrobial, Antinociceptive, and Anti-Inflammatory Activities of Two Chemotypes of Pimenta pseudocaryophyllus (Myrtaceae).

    Science.gov (United States)

    de Paula, Joelma Abadia Marciano; Silva, Maria do Rosário Rodrigues; Costa, Maysa P; Diniz, Danielle Guimarães Almeida; Sá, Fabyola A S; Alves, Suzana Ferreira; Costa, Elson Alves; Lino, Roberta Campos; de Paula, José Realino

    2012-01-01

    Preparations from Pimenta pseudocaryophyllus (Gomes) L.R. Landrum (Myrtaceae) have been widely used in Brazilian folk medicine. This study aims to evaluate the antimicrobial activity of the crude ethanol extracts, fractions, semipurified substances, and essential oils obtained from leaves of two chemotypes of P. pseudocaryophyllus and to perform the antinociceptive and anti-inflammatory screening. The ethanol extracts were purified by column chromatography and main compounds were spectrally characterised (1D and 2D (1)H and (13)C NMR). The essential oils constituents were identified by GC/MS. The broth microdilution method was used for testing the antimicrobial activity. The abdominal contortions induced by acetic acid and the ear oedema induced by croton oil were used for screening of antinociceptive and anti-inflammatory activities, respectively. The phytochemical analysis resulted in the isolation of pentacyclic triterpenes, flavonoids, and phenol acids. The oleanolic acid showed the best profile of antibacterial activity for Gram-positive bacteria (31.2-125 μg mL(-1)), followed by the essential oil of the citral chemotype (62.5-250 μg mL(-1)). Among the semipurified substances, Ppm5, which contained gallic acid, was the most active for Candida spp. (31.2 μg mL(-1)) and Cryptococcus spp. (3.9-15.6 μg mL(-1)). The crude ethanol extract and fractions from citral chemotype showed antinociceptive and anti-inflammatory effects.

  17. Phytochemical Analysis and Antimicrobial, Antinociceptive, and Anti-Inflammatory Activities of Two Chemotypes of Pimenta pseudocaryophyllus (Myrtaceae

    Directory of Open Access Journals (Sweden)

    Joelma Abadia Marciano de Paula

    2012-01-01

    Full Text Available Preparations from Pimenta pseudocaryophyllus (Gomes L.R. Landrum (Myrtaceae have been widely used in Brazilian folk medicine. This study aims to evaluate the antimicrobial activity of the crude ethanol extracts, fractions, semipurified substances, and essential oils obtained from leaves of two chemotypes of P. pseudocaryophyllus and to perform the antinociceptive and anti-inflammatory screening. The ethanol extracts were purified by column chromatography and main compounds were spectrally characterised (1D and 2D 1H and 13C NMR. The essential oils constituents were identified by GC/MS. The broth microdilution method was used for testing the antimicrobial activity. The abdominal contortions induced by acetic acid and the ear oedema induced by croton oil were used for screening of antinociceptive and anti-inflammatory activities, respectively. The phytochemical analysis resulted in the isolation of pentacyclic triterpenes, flavonoids, and phenol acids. The oleanolic acid showed the best profile of antibacterial activity for Gram-positive bacteria (31.2–125 μg mL−1, followed by the essential oil of the citral chemotype (62.5–250 μg mL−1. Among the semipurified substances, Ppm5, which contained gallic acid, was the most active for Candida spp. (31.2 μg mL−1 and Cryptococcus spp. (3.9–15.6 μg mL−1. The crude ethanol extract and fractions from citral chemotype showed antinociceptive and anti-inflammatory effects.

  18. Essential Oils of Hyptis pectinata Chemotypes: Isolation, Binary Mixtures and Acute Toxicity on Leaf-Cutting Ants.

    Science.gov (United States)

    Feitosa-Alcantara, Rosana B; Bacci, Leandro; Blank, Arie F; Alves, Péricles B; Silva, Indira Morgana de A; Soares, Caroline A; Sampaio, Taís S; Nogueira, Paulo Cesar de L; Arrigoni-Blank, Maria de Fátima

    2017-04-12

    Leaf-cutting ants are pests of great economic importance due to the damage they cause to agricultural and forest crops. The use of organosynthetic insecticides is the main form of control of these insects. In order to develop safer technology, the objective of this work was to evaluate the formicidal activity of the essential oils of two Hyptis pectinata genotypes (chemotypes) and their major compounds on the leaf-cutting ants Acromyrmex balzani Emery and Atta sexdens rubropilosa Forel. Bioassays of exposure pathways (contact and fumigation) and binary mixtures of the major compounds were performed. The major compounds identified in the essential oils of H. pectinata were β-caryophyllene, caryophyllene oxide and calamusenone. The essential oils of H. pectinata were toxic to the ants in both exposure pathways. Essential oils were more toxic than their major compounds alone. The chemotype calamusenone was more toxic to A. balzani in both exposure pathways. A. sexdens rubropilosa was more susceptible to the essential oil of the chemotype β-caryophyllene in both exposure pathways. In general, the binary mixtures of the major compounds resulted in additive effect of toxicity. The essential oils of H. pectinata is a raw material of great potential for the development of new insecticides.

  19. Metabolic profiling for studying chemotype variations in Withania somnifera (L.) Dunal fruits using GC-MS and NMR spectroscopy.

    Science.gov (United States)

    Bhatia, Anil; Bharti, Santosh K; Tewari, Shri K; Sidhu, Om P; Roy, Raja

    2013-09-01

    Withania somnifera (L.) Dunal (Solanaceae), commonly known as Ashwagandha, is one of the most valued Indian medicinal plant with several pharmaceutical and nutraceutical applications. Metabolic profiling was performed by GC-MS and NMR spectroscopy on the fruits obtained from four chemotypes of W. somnifera. A combination of (1)H NMR spectroscopy and GC-MS identified 82 chemically diverse metabolites consisting of organic acids, fatty acids, aliphatic and aromatic amino acids, polyols, sugars, sterols, tocopherols, phenolic acids and withanamides in the fruits of W. somnifera. The range of metabolites identified by GC-MS and NMR of W. somnifera fruits showed various known and unknown metabolites. The primary and secondary metabolites observed in this study represent MVA, DOXP, shikimic acid and phenylpropanoid biosynthetic metabolic pathways. Squalene and tocopherol have been rated as the most potent naturally occurring compounds with antioxidant properties. These compounds have been identified by us for the first time in the fruits of W. somnifera. Multivariate principal component analysis (PCA) on GC-MS and NMR data revealed clear distinctions in the primary and secondary metabolites among the chemotypes. The variation in the metabolite concentration among different chemotypes of the fruits of W. somnifera suggest that specific chemovars can be used to obtain substantial amounts of bioactive ingredients for use as potential pharmacological and nutraceuticals agents.

  20. IDENTIFICATION AND CHARACTERIZATION OF A NEW CHEMOTYPE OF NON-COVALENT SENP INHIBITORS

    Science.gov (United States)

    Madu, Ikenna G.; Namanja, Andrew T.; Su, Yang; Wong, Steven; Li, Yi-Jia; Chen, Yuan

    2013-01-01

    Enzymes called SENPs catalyze both the maturation of small ubiquitin-like modifier (SUMO) precursors and removal of SUMO modifications, which regulate essential cellular functions such as cell cycle progression, DNA damage response and intracellular trafficking. Some members, such as SENP1, are potential targets for developing cancer therapeutics. We searched for small molecule inhibitors of SENPs using in-silico screening in conjunction with biochemical assays, and identified a new chemotype of small molecule inhibitors that non-covalently inhibit SENPs. The inhibitors confer the non-competitive inhibitory mechanism, as shown by nuclear magnetic resonance (NMR) and quantitative enzyme kinetic analysis. The NMR data also provided evidence for substrate-assisted inhibitor binding, which indicates the need for caution in using artificial substrates for compound screening, as the inhibitory effects could be significantly different from using the physiological substrates. This finding also suggests the possibility of designing inhibitors for this class of enzymes that are tuned for substrate-specificity. PMID:23614497

  1. Avaliação da toxicidade subcrônica do extrato bruto seco de Anacardium occidentale Linn em cães = Evaluation of the subchronic toxicity of the crude dry extract of Anacardium occidentale Linn in dogs

    Directory of Open Access Journals (Sweden)

    Arquimedes Fernandes Monteiro de Melo

    2006-01-01

    Full Text Available A busca de novos medicamentos tem levado ao desenvolvimento de novosfármacos que sejam eficientes e destituídos de toxicidade. Uma das fronteiras nessas pesquisas são os medicamentos fitoterápicos. No Brasil, a Agência Nacional de Vigilância Sanitária (ANVISA regulariza essas pesquisas e padroniza os procedimentos. A Resoluçãoda Diretoria Colegiada (RDC 48/2004, por exemplo, regulariza o registro de fitoterápicos. O Anacardium occidentale Linn está entre as plantas mais estudadas, devido às ações antibiótica e antiinflamatória de seus metabólitos secundários, principalmente taninos. Esta planta também possui a capacidade de impedir a formação da placa bacteriana bucal. Diante dessas ações, formas farmacêuticas acabadas (cremes e géis foram desenvolvidas a partir do extrato bruto seco (EBS das cascas do caule do A. occidentale Linn para registro de um novo fitomedicamento. Entretanto, testes pré-clínicos e clínicos devem ser feitos de acordo com a lei vigente. O presente trabalho avaliou a toxicidade subcrônica do EBS em cães sem raçadefinida (SRD. Os testes revelaram apenas hepatotoxicidade transitória demonstrada pela elevação dos níveis da alanina transaminase (ALT e aspartato transaminase (AST. Research on new medicaments has led to the development of efficient and non-toxic drugs. In Brazil, the Agência Nacional de VigilânciaSanitária (National Department of Sanitary Supervision – ANVISA regularizes and standardizes the procedure. Anacardium occidentale is amongst the most researched plants, due to the antibiotics and antinflammatory properties of its secondary metabolites, mainlytannins and flavonoids. Furthermore, it prevents the dental plaque formation. On account of these actions, finished pharmaceutical forms (creams and gels were developed from the crude dry extract (CDE of A. occidentale Linn stem rinds, in order to register a new form.However, pre-clinical and clinical assays can be made in

  2. Avaliação da toxicidade subcrônica do extrato bruto seco de Anacardium occidentale Linn em cães = Evaluation of the subchronic toxicity of the crude dry extract of Anacardium occidentale Linn in dogs

    OpenAIRE

    Arquimedes Fernandes Monteiro de Melo; Miracy Muniz Albuquerque; Maria Almerice Lopes da Silva; Gracielle Carvalho Gomes; Isabely de Souza Vera Cruz; Vanessa Ribeiro Leite; Jane Sheila Higino

    2006-01-01

    A busca de novos medicamentos tem levado ao desenvolvimento de novosfármacos que sejam eficientes e destituídos de toxicidade. Uma das fronteiras nessas pesquisas são os medicamentos fitoterápicos. No Brasil, a Agência Nacional de Vigilância Sanitária (ANVISA) regulariza essas pesquisas e padroniza os procedimentos. A Resoluçãoda Diretoria Colegiada (RDC) 48/2004, por exemplo, regulariza o registro de fitoterápicos. O Anacardium occidentale Linn está entre as plantas mais estudadas, devido às...

  3. Evaluation of the antinociceptive and anti-inflammatory effects of the acetone extract from Anacardium occidentale L

    Directory of Open Access Journals (Sweden)

    Frederico Argollo Vanderlinde

    2009-09-01

    Full Text Available The stem bark of Anacardium occidentale L. (Anacardiaceae, commonly called cashew, is used in Brazilian traditional medicine for the treatment of gastric and inflammatory disorders. The present study was carried out to investigate the in vivo anti-inflammatory activities of the acetone extract (AE of the stem bark of A. occidentale. We evaluated the pharmacological activities of this plant material through the analgesic, antiedematogenic and chemotaxic inhibitory effects produced by the AE. The oral administration (p.o. of mice with the AE (0.1, 0.3 and 1.0 g/kg or positive control indomethacin (10 mg/kg inhibited acetic acid-induced writhing by 18.9, 35.9, 62.9 and 68.9%, respectively (ID50% = 530 mg/kg. The highest dose of the AE was able to inhibit croton oil-induced ear edema formation by 56.8% (indomethacin at 10 mg/kg, p.o. - 57.6% inhibition. When submitted to the carrageenan-induced peritonitis test, the AE (0.1, 0.3 and 1.0 g/kg, p.o. impaired leukocyte migration into the peritoneal cavity by 24.8, 40.5 and 49.6%, respectively. The positive control, dexamethasone (2 mg/kg, s.c., inhibited leukocyte migration by 66.9%. These results indicate the presence of anti-inflammatory and antinociceptive principles in the acetone extract of Anacardium occidentale, and reinforce the plant's potential therapeutic use against pain and inflammatory diseases.As cascas do caule do Anacardium occidentale L. (Anacardiaceae, conhecido como cajueiro, são popularmente utilizadas no Brasil para o tratamento de doenças gástricas e inflamatórias. Este estudo teve como objetivo a avaliação farmacológica in vivo da atividade antiinflamatória do extrato acetônico (AE obtido das cascas do A. occidentale, investigando os efeitos analgésico, antiedematogênico e inibitório sobre a quimiotaxia deste material botânico. A administração oral (p.o. em camundongos com o AE (0,1; 0,3 e 1 g/kg ou o controle positivo indometacina (10 mg/kg inibiu as contor

  4. In vitro antimicrobial and cytotoxic effects of Anacardium occidentale and Mangifera indica in oral care

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    Geethashri Anand

    2015-01-01

    Full Text Available Background: Oral health is an integral and important component of general health. Infectious diseases such as caries, periodontal, and gingivitis indicate the onset of imbalance in homeostasis between oral micro biota and host. The present day medicaments used in oral health care have numerous side effects. The uses of herbal plants as an alternative have gained popularity due to side effects of antibiotics and emergence of multidrug resistant strains. Anacardium occidentale (cashew and Mangifera indica (mango have been used as traditional oral health care measures in India since time immemorial. Materials and Methods: The ethanol extracts of cashew and mango leaves were obtained by maceration method. The antimicrobial activity was evaluated by clear zone produced by these plant extracts against Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, and Candida albicans in agar plate method, determination of minimum inhibitory concentration (MIC, minimum bactericidal/fungicidal concentration (MBC/MFC, and suppression of biofilm. The cytotoxic effects of plants extract was determined by microculture tetrazolium assay on human gingival fibroblast and Chinese hamster lung fibroblast (V79 cell lines. Results: Cashew and mango leaf extract significantly (P < 0.05 produced larger zone of inhibition against test pathogens when compared to povidone---iodine-based mouth rinses. Although the MIC and MBC/MFC values of mouth rinses were effective in lower concentrations; plant extracts significantly (P < 0.001 suppressed the biofilms of oral pathogens. The leaf extracts were less cytotoxic (P < 0.001 compared to mouth rinses. Conclusions: Plant extracts are superior to the mouth rinses and have a promising role in future oral health care.

  5. Mountain ungulates and the conservation of scarce plant species: the cases of Delphinium montanum and Xatardia scabra

    Directory of Open Access Journals (Sweden)

    Aymerich, P.

    2002-12-01

    Full Text Available In order to assess the possible negative influence of a high density population ofizard (Rupicapra pyrenaica on the occurrence of several restricted range plants in the eastern Pyrenees, a monitoring experiment was carried out in the Cadí- Moixeró Natural Park and Game Reserve. Ungulates prédation upon reproductive parts of the plants is rejected as a factor influencing Delphinium montanum and Xatardia scabra population viability, even when high rates of consumption of flowers and fresh fruits are detected. Population dynamics of both plant species are mainly regulated by the habitat physical conditions and by intraspecific competition. Plantlet occurrence depends on the availability of bare substrate and is not related to seed production, nor the presence of ungulates. Plant density and age structure for these scree species rely on the limited availability of substrate resources, which leads to strong intraspecific competition.

    [fr]
    Une étude de suivi a été développée dans le Parc naturel et Réserve de Chasse du Cadí-Moixeró (Catalogne, Espagne. Le but était de tester la possible influence négative de la haute densité de population des isards (Rupicapra pyrenaica sur la présence de quelques plantes à aire de répartition réduite dans les Pyrénées- Orientales. La prédation des Ongulés sur les parties reproductives des plantes n'est pas un facteur agissant sur la viabilité des populations de Delphinium montanum et Xatardia scabra et cela même s'ils arrivent à consommer beaucoup de fleurs et de fruits frais. La dynamique des populations des deux plantes semble régulée par les conditions physiques de l'habitat et par la concurrence intraspécifique. La présence de plantules est en rapport avec la disponibilité de sol nu et se montre indépendante de la production de semences, l'impact des ongulés étant nul. Tant la densité que la structure d'âges de ces plantes

  6. Del arte común de curar a España y a las Indias occidentales

    OpenAIRE

    Estela Restrepo Zea

    2010-01-01

    Desde los primeros viajes colombinos, cronistas, soldados y otros viajeros llevaron hasta Europa noticias sobre las plantas americanas acompañadas de fabulosos relatos. Como en "Las palabras y las cosas", texto que para Foucault nació a partir del libro de Borges, de ese que cita "cierta enciclopedia china", así parecen haber entrado en el discurso de la historia natural, los seres y las cosas de las Indias occidentales desde el primer viaje de Colón. Seres que se volvieron "familiares en el ...

  7. Del arte común de curar a España y a las Indias occidentales

    OpenAIRE

    Estela Restrepo Zea

    1997-01-01

    Desde los primeros viajes colombinos, cronistas, soldados y otros viajeros llevaron hasta Europa noticias sobre las plantas americanas acompañadas de fabulosos relatos. Como en "Las palabras y las cosas", texto que para Foucault nació a partir del libro de Borges, de ese que cita "cierta enciclopedia china", así parecen haber entrado en el discurso de la historia natural, los seres y las cosas de las Indias occidentales desde el primer viaje de Colón. Seres que se volvieron "familiares en el ...

  8. Alterações químicas e físico-químicas no processamento de suco de caju (Anacardium occidentale L. Physicochemical changes in cashew apple (Anacardium occidentale L. Juice processing

    Directory of Open Access Journals (Sweden)

    Soraya de Oliveira Sancho

    2007-12-01

    Full Text Available O presente trabalho teve como objetivo determinar as alterações físico-químicas do suco de caju (Anacardium occidentale L. com alto teor de polpa, em diferentes etapas do seu processamento industrial. Observou-se que a acidez e o teor de ácido ascórbico do suco diminuíram progressivamente com o processamento industrial, exibindo os menores teores na etapa de pasteurização. Os teores de ácido fólico apresentaram comportamento diferenciado, apresentando os maiores teores na etapa de homogeneização do suco. Os valores de açúcares redutores, não-redutores e totais, assim como o pH, se mantiveram constantes.This work aimed to study the physicochemical changes occurring in cashew apple (Anacardium occidentale L. juice with high pulp content in various stages of the industrial process. The acidity and ascorbic acid contents were found to decrease progressively along the process, presenting their lowest values in the pasteurization stage. Folic acid content was highest in the juice homogenizing stage. Reducing and non-reducing sugars, as well as pH values remained constant throughout the process.

  9. Trichothecene genotypes and chemotypes in Fusarium graminearum complex strains isolated from maize fields of northwest Argentina.

    Science.gov (United States)

    Sampietro, Diego A; Ficoseco, María E Aristimuño; Jimenez, Cristina M; Vattuone, Marta A; Catalán, Cesar A

    2012-02-01

    Members of the Fusarium graminearum species complex (Fg complex) cause Gibberella ear rot in maize from northwest Argentina. The potential of these pathogens to contaminate maize grains with type B trichothecenes is a health risk for both humans and animals. We evaluated the reliability of multiplex PCR assays based on TRI3 and TRI12 genes, and single PCR assays based on TRI7 and TRI13 genes to infer trichothecene chemotypes of 112 strains of Fg complex collected from northwest Argentina, checking trichothecene production by chemical analysis. Single and multiplex PCR assays indicated that strains belonging to F. meridionale (87/112) had a NIV genotype. The remainder strains (25/112), which belonged to F. boothii, had a DON genotype (based on single PCR assays) or 15ADON genotype (based on multiplex PCR assays). No strains tested were incorrectly diagnosed with a DON/NIV genotype. Chemical analysis indicated that the F. meridionale strains were NIV producers only (44/87), major NIV producers with unexpected high DON/NIV ratios (36/87), or unexpected major DON producers with minor NIV production (7/87). Strains with atypical DON/NIV production seem to be new phenotypes under a putative NIV genotype, since PCR assays do not provide evidences of a new trichothecene genotype. DON production and absence of its acetylated forms were shown for strains of F. boothii. The inconsistencies between genetic and chemical data highlight the risk of inferring the trichothecenes potentially contaminating food and feedstuffs based only on PCR assays. This study confirms for the first time that strains of Fg complex from maize of northwest Argentina are DON and NIV producers. In addition, dominance of NIV producers in the Fg complex population isolated from maize is unprecedented in Argentina, and of significant concern to food safety and animal production.

  10. Radioprotective effect of the extract of Ziziphus joazeiro and Anacardium occidentale on embryos of Biomphalaria glabrata submitted to ionizing radiation

    Energy Technology Data Exchange (ETDEWEB)

    Siqueira, Williams N.; Silva, Luanna R.S.; Silva, Edvane B. [Universidade Federal de Pernambuco (DEN/UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Grupo de Estudos em Radioprotecao e Radioecologia; Silva, Ronaldo C. [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Genetica; Lacerda, Laila B.N.; Silva, Hianna A.M.F.; Santos, Mariana L.O.; Sa, Jose L.F.; Melo, Ana M.M.A. [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de de Biofisica e Radiobiologia. Lab. de Radiobiologia

    2011-07-01

    Electromagnetic radiations are energies that can be classified as non-ionizing and ionizing. This type of energy is propagated by a material medium and the vacuum. The important characteristic of ionizing radiation is the localized release of large amounts of energy. The biological effects of radiation result principally from damage to DNA, which is the critical target. Given these harmful effects caused by radiation highlights the importance of acquiring knowledge about the radioprotective substance, because they act to protect the living tissue, decreasing the damage he caused by the effects of radiation. In this study we investigated the radioprotective effect of extract hydroalcoholic of Ziziphus joazeiro and Anacardium occidentale on embryos of Biomphalaria glabrata. The embryos of Biomphalaria glabrata pigmented were divided into 18 groups of 100 specimens. The experimental groups were exposed to the extracts at a concentration of 200 ppm and then irradiated. For irradiation, we used a source of {sup 60}Co (Gammacell of Radionics Labs. Dose rate = 4.359 Gy/h). The viability of the embryos was examined using a stereoscopic microscope and statistical analysis was performed using the test Student-Newman-Keuls and {chi}{sup 2}. Our results showed that the extracts of hydroalcoholic Ziziphus joazeiro showed radioprotective effect and that the aqueous extract of the bark of Anacardium occidentale exhibited a reduction in its embryotoxic effect. (author)

  11. ¹H NMR and HPLC/DAD for Cannabis sativa L. chemotype distinction, extract profiling and specification.

    Science.gov (United States)

    Peschel, Wieland; Politi, Matteo

    2015-08-01

    The medicinal use of different chemovars and extracts of Cannabis sativa L. requires standardization beyond ∆9-tetrahydrocannabinol (THC) with complementing methods. We investigated the suitability of (1)H NMR key signals for distinction of four chemotypes measured in deuterated dimethylsulfoxide together with two new validated HPLC/DAD methods used for identification and extract profiling based on the main pattern of cannabinoids and other phenolics alongside the assayed content of THC, cannabidiol (CBD), cannabigerol (CBG) their acidic counterparts (THCA, CBDA, CBGA), cannabinol (CBN) and cannflavin A and B. Effects on cell viability (MTT assay, HeLa) were tested. The dominant cannabinoid pairs allowed chemotype recognition via assignment of selective proton signals and via HPLC even in cannabinoid-low extracts from the THC, CBD and CBG type. Substantial concentrations of cannabinoid acids in non-heated extracts suggest their consideration for total values in chemotype distinction and specifications of herbal drugs and extracts. Cannflavin A/B are extracted and detected together with cannabinoids but always subordinated, while other phenolics can be accumulated via fractionation and detected in a wide fingerprint but may equally serve as qualitative marker only. Cell viability reduction in HeLa was more determined by the total cannabinoid content than by the specific cannabinoid profile. Therefore the analysis and labeling of total cannabinoids together with the content of THC and 2-4 lead cannabinoids are considered essential. The suitability of analytical methods and the range of compound groups summarized in group and ratio markers are discussed regarding plant classification and pharmaceutical specification.

  12. HOMO VIATOR – O DIMENSIUNE FUNDAMENTALĂ A SPIRITUALITĂŢII OCCIDENTALE

    Directory of Open Access Journals (Sweden)

    Tatiana CIOCOI

    2015-12-01

    Full Text Available Articolul de faţă reflectă modificările fundamentale care s-au produs în mentalitatea occidentală contemporană prin intermediul conceptului de călător (homo viator. Noţiunea de călător este tratată în sens culturologic larg drept  desco­peritor de lumi necunoscute, explorator, navigator sau aventurier al oricărui tip de cunoaştere, care reprezintă trăsătura fundamentală a omului occidental. Prototipul absolut al acestui model cultural îl reprezintă Ulise, eroul din Odiseea lui Homer. Conceptul de „călătorie” oferă, astfel, o viziune transversală asupra evoluţiei literaturii occidentale, al cărei centru iradiant – tensiunea cunoaşterii – a problematizat cele mai variate forme de călătorie şi de călător. Istoria romanului occidental este urmărit dea lungul secolelor şi a epocilor literare pentru a observa cum s-a modificat percepţia călătoriei, iar odată cu ea, şi spiritualitatea occidentală. Viziunea contemporană asupra călătoriei este analizată în baza romanului lui Michel Houellebecq „Harta şi teritoriul” (2010. HOMO VIATOR – A FUNDAMENTAL DIMENSION  OF WESTERN SPIRITUALITYThe article reflects the changes produced in the western contemporary mentality through the concept of the traveler (homo viator. The notion of traveler is approached in wide cultural meaning as a discoverer of unknown worlds, explorer, navigator or adventurer of every type of knowing, that represents the fundamental feature of the occidental man. The absolute prototype of this cultural model is represented by Ulysses, the hero of Homer’s Odyssey. The concept of “travel” offers thus a transverse vision upon the evolution of the occidental literature whose irradiant centre – the tension of knowing – discussed the problems of the most varied forms of travel and traveler. The history of the western novel is followed over centuries and literary epochs in order to observe which way the perception of travel

  13. Tecniche costruttive e forme di potere nella Toscana sud-occidentale (secc. VIII-XIV

    Directory of Open Access Journals (Sweden)

    Bianchi, Giovanna

    2005-12-01

    Full Text Available This paper tells about the analysis of the building techniques of elevations inferred from data obtained in extensive projects of archaeological research executed in western Tuscany's rural field from half '90s of last century to present. The text looks over the changes from wooden building trade related to the first high rise habitants in VIIth-VIIIth century, until the pattern settlements in the second half of VIIIth and XIth centuries, characterized by the first use of masonry and the presence of expert master builders. The more complex organization of the building workshops for castles of the XIth and XIIth centuries in relation with the liege lord's rising politic abusive authority is explained in the following part. The subsequent formation of new suburbs between the XIIIth and the XIV centuries is characterized by a different way of use of building techniques, often founded over pre-existing castles, linked to local council, up to the political and economical influence of Pisa in this territory.Nell’articolo si tratta l’analisi delle tecniche murarie desunta da dati provenienti da ampi progetti di indagine archeologica svolti in ambito rurale nella Toscana occidentale dalla metà degli anni Novanta dello scorso secolo ad oggi. Nel testo si esaminano i cambiamenti dei modi di edificare a partire dall’edilizia in legno dei primi abitati di altura di VII-VIII secolo, sino agli insediamenti più strutturati di seconda metà VIII e IX secolo, caratterizzati da un primo uso della pietra e dalla presenza di maestranze specializzate. In seguito si analizza la più complessa organizzazione del cantiere propria della costruzione dei castelli di XI e XII secolo in rapporto ai poteri politici ed economici delle nascenti signorie territoriali. Un differente uso delle tecniche costruttive caratterizza la successiva formazione di nuovi borghi tra XIII e XIV secolo, spesso impiantati sui preesistenti castelli, legati ai locali organismi comunali

  14. Image Annotation and Database Mining to Create a Novel Screen for the Chemotype-Dependent Crystallization of HCV NS3 Protease

    Energy Technology Data Exchange (ETDEWEB)

    H Klei; K Kish; M Russo; S Michalczyk; M Cahn; J Tredup; C Chang; J Khan; E Baldwin

    2011-12-31

    An effective process for screening, imaging, and optimizing crystallization trials using a combination of external and internal hardware and software has been deployed. The combination of this infrastructure with a vast annotated crystallization database enables the creation of custom crystallization screening strategies. Because of the strong chemotype-dependent crystallization observed with HCV NS3 protease (HCVPr), this strategy was applied to a chemotype resistant to all prior crystallization efforts. The crystallization database was mined for ingredients used to generate earlier HCVPr/inhibitor co-crystals. A random screen was created from the most prolific ingredients. A previously untested combination of proven ingredients was identified that led to a successful crystallization condition for the resistant chemotype.

  15. Comparative characterization of Santolina insularis chemotypes by essential oil composition, 5S-rRNA-NTS sequencing and EcoRV RFLP-PCR.

    Science.gov (United States)

    Gnavi, Giorgio; Bertea, Cinzia M; Usai, Marianna; Maffei, Massimo E

    2010-06-01

    Santolina insularis (Genn ex Fiori) Arrig. is a medicinal plant whose essential oil shows antiviral and antibacterial activities and potent and selective cytotoxic activity against the human colon carcinoma cell line. The occurrence of several chemotypes makes the taxonomic identification of S. insularis hard to achieve. GC-MS essential oil analyses of four chemotypes (SI1, SI2, SI3 and SI4) revealed the presence of different percentages of santolina triene, beta-pinene, myrcene, beta-phellandrene, artemisia ketone and cis-chrysanthemol, allowing a chemical discrimination. Single fragments of the 5S-rRNA-NTS region of approximately 150, 170, 260 and 280bp were produced by SI1, SI2, SI3 and SI4, respectively, and the sequence alignment of the 5S-rRNA spacer region flanked by the 3'-and 5'-ends of the coding region confirmed a consistent difference between chemotypes. Furthermore, a PCR-RFLP method was applied. From the identified sequences, an EcoRV site could be found in chemotypes SI1, SI2 and SI3 in the 5S-rRNA spacer regions at 81 bp position; however, this site was absent in the chemotype SI4. This study, by showing remarkable chemical variation in the terpenoid profile and consistent genomic difference in the 5S-rRNA spacer regions, identified four chemotypes of S. insularis which could be grouped into two ecotypes, based on chemical and genomic analyses. The identification of specific gene sequences of the 5S-rRNA-NTS region and of a EcoRV site identified in this work can be used for a rapid and precise identification of the plant chemo-/ecotypes, complementing the essential oil chemical analysis. Copyright 2010 Elsevier Ltd. All rights reserved.

  16. Utilisation of Cashew Nut Shell Liquid from Anacardium occidentale as Starting Material for Organic Synthesis: A Novel Route to Lasiodiplodin from Cardols

    OpenAIRE

    Santos, Maria Lucilia dos; Magalhães,Gouvan C. de

    1999-01-01

    As part of an ongoing program concerning utilisation of CNSL (Cashew Nut Shell Liquid) from Anacardium occidentale as starting material for the preparation of useful compounds, we describe the conversion of cardols (6-alkenylresorcinols) into lasiodiplodin, a naturally occurring 12-membered orsellinic acid type macrolide, which exhibits plant growth regulating and antileukemic properties.

  17. Comparative Analysis of Deoxynivalenol Biosynthesis Related Gene Expression among Different Chemotypes of Fusarium graminearum in Spring Wheat

    Science.gov (United States)

    Amarasinghe, Chami C.; Fernando, W. G. Dilantha

    2016-01-01

    Fusarium mycotoxins, deoxynivalenol (DON) and nivalenol (NIV) act as virulence factors and are essential for symptom development after initial infection in wheat. To date, 16 genes have been identified in the DON biosynthesis pathway. However, a comparative gene expression analysis in different chemotypes of Fusarium graminearum in response to Fusarium head blight infection remains to be explored. Therefore, in this study, nine genes that involved in trichothecene biosynthesis were analyzed among 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON) and nivalenol producing F. graminearum strains in a time course study. Quantitative reverse transcription polymerase chain reaction revealed that the expression of all examined TRI gene transcripts initiated at 2 days post-inoculation (dpi), peaked at three to four dpi and gradually decreased at seven dpi. The early induction of TRI genes indicates that presence of high levels of TRI gene transcripts at early stages is important to initiate the biosynthetic pathway of DON and NIV. Comparison of gene expression among the three chemotypes showed that relative expression of TRI genes was higher in 3-ADON producing strains compared with 15-ADON and NIV strains. Comparatively higher levels of gene expression may contribute to the higher levels of DON produced by 3-ADON strains in infected grains. PMID:27550207

  18. Fenóis totais, atividade antioxidante e constituintes químicos de extratos de Anacardium occidentale L., Anacardiaceae Total phenolics, antioxidant activity and chemical constituents from extracts of Anacardium occidentale L., Anacardiaceae

    Directory of Open Access Journals (Sweden)

    Mariana H. Chaves

    2010-03-01

    Full Text Available O presente artigo relata o isolamento e identificação do palmitato, oleato e linoleato de sitosterila, sitosterol, estigmasterol, 3-O-β-D-galactopiranosídeo do sitosterol, 3-O-β-Dgalactopiranosídeo do estigmasterol, 3-O-β-D-glicopiranosídeo do sitosterol e uma mistura de ácidos anacárdicos (monoeno e dieno do extrato etanólico de cascas do caule de Anacardium occidentale L., Anacardiaceae, bem como do sitosterol, estigmasterol, lupeol, β-amirina, catequina e epicatequina do extrato etanólico do tegumento da castanha de caju in natura. Os extratos EtOH da casca e do tegumento foram avaliados quanto ao conteúdo de fenóis totais e atividade antioxidante. O extrato etanólico das cascas do caule apresentou maior conteúdo de compostos fenólicos e percentual de atividade antioxidante.This paper describes the isolation and identification of a mixture of sitosteryl ester derivatives of fatty acids (palmitic, oleic and linoleic, sitosterol, stigmasterol, sitosterol-3-O-β-galactopyranoside, stigmasterol-3-O-β-galactopyranoside, sitosterol-3-O-β-glucopyranoside and a mixture of anacardic acids (monoene and diene from stem bark of Anacardium occidentale L., Anacardiaceae, as well as sitosterol, stigmasterol, lupeol, β-amyrin, catechin and epicatechin from in natura cashew nut testa. Ethanol extracts from stem bark and testa were analyzed for antioxidant activity and total phenol content. The ethanol extract from stem bark exhibited the maximum of antioxidant activity and phenol content.

  19. Atividade antimicrobiana in vitro do extrato de Anacardium occidentale L. sobre espécies de Streptococcus In vitro antimicrobial activity of an extract of Anacardium occidentale L. against Streptococcus species

    Directory of Open Access Journals (Sweden)

    Arquimedes F. Monteiro de Melo

    2006-06-01

    Full Text Available A atividade antimicrobiana do extrato da casca do caule de Anacardium occidentale L., foi avaliada em três culturas de bactérias isoladas de biofilme dental. A atividade antimicrobiana foi conduzida em placa de Petri pelo método de difusão para determinação da Concentração Inibitória Mínima (CIM e Concentração Inibitória Mínima de Aderência (CIMA. Inibição aureolar (CIM foi observada com concentrações de 12,5 mg/mL em S. mutans e 6,25mg/mL em S. mitis e S. sanguis. Os resultados sugerem que o extrato tem efeito na CIMA em concentrações de 0,31 mg/mL em S. mutans e S. mitis e de 0,15 mg/mL em S. sanguis e pode ser usado terapeuticamente na odontologia como agente antibacteriano.The antimicrobial activity of the extract of the stem bark of Anacardium occidentale L., was evaluated in three cultures of bacteria of the dental plate. The antimicrobial activity was assayed in Petri dishes using the diffusion method for determination of the minimal inhibitory concentration (MIC and minimal inhibitory concentration of adherence (MICA. Aureolar inhibition (MIC was observed with concentrations of 12.5 mg/mL in S. mutans and 6.25 mg/mL in S. mitis and S. sanguis. The results suggest that the extract has effect in the MICA in concentrations of 0.31 mg/mL in S. mutans and S. mitis and of 0.15 mg/mL in S. sanguis and therapeuticly can be used in the dentistry as an antibacterial agent.

  20. Brachistosternus ninapo una nueva especie (Scorpiones:Bothriuridae de los Andes occidentales en el sur del Perú

    Directory of Open Access Journals (Sweden)

    José Antonio Ochoa

    2013-06-01

    Full Text Available Se describe Brachistosternus ninapo, nueva especie de Bothriuridae de los Andes occidentales del sur del Perú (2900 a 4050 m. Esta especie pertenece al subgénero Brachistosternus (Leptosternus Maury. Está relacionada con la especie boliviana Brachistosternus galianoae Ojanguren Affilastro y se diferencia de ella, por la morfología del hemiespermatóforo (forma de la lámina y algunos detalles de la región de lóbulos, el número de setas dorsales laterales del segmento caudal V, la proporción largo/ancho de la pinza del pedipalpo y en el patrón de pigmentación de los tergitos.

  1. Aktivitas Zymomonas mobilis pada produk etanol dari buah semu jambu mete (Anacardium occidentale dengan variasi sumber nitrogen

    Directory of Open Access Journals (Sweden)

    AKHMAD MUSTOFA

    2010-05-01

    Full Text Available Mustofa A, Suranto. 2010. Aktivitas Zymomonas mobilis pada produk etanol daribuah semu jambu mete (Anacardium occidentale dengan variasi sumber nitrogen. Bioteknologi 7: 1-9. Penelitian ini bertujuan mengetahui kemampuan Zymomonas mobilis dalam memproduksi etanol melalui proses fermentasi batch (selama 24, 48 dan 72 jam, menggunakan sumber karbon sari buah jambu mete (varietas merah, hijau dan kuning dan sumber nitrogen berupa urea, ammonium sulfat, ekstrak kecambah kacang hijau dan ekstrak kacang koro (Mucuna pruriens. Hasil penelitian menunjukkan bahwa varietas buah jambu mete hijau dengan sumber nitrogen ammonium sulfat dan lama fermentasi 24 jam memberikan hasil etanol yang paling optimal. Pada perlakuan tersebut diperoleh nilai pH 5,87, kadar gula reduksi 7,64 g/100 mL (tingkat konsumsi 48,44%, jumlah bakteri 8,0x107 (µ = 0,154 dan etanol sebesar 33,02 g/L (Ye = 90,19%.

  2. Del arte común de curar a España y a las Indias occidentales

    Directory of Open Access Journals (Sweden)

    Estela Restrepo Zea

    2010-07-01

    Full Text Available Desde los primeros viajes colombinos, cronistas, soldados y otros viajeros llevaron hasta Europa noticias sobre las plantas americanas acompañadas de fabulosos relatos. Como en "Las palabras y las cosas", texto que para Foucault nació a partir del libro de Borges, de ese que cita "cierta enciclopedia china", así parecen haber entrado en el discurso de la historia natural, los seres y las cosas de las Indias occidentales desde el primer viaje de Colón. Seres que se volvieron "familiares en el Viejo Mundo, como a su edad y a su geografía". / incluye fragmento de un antidotario del siglo XVII en Cartagena de Indias. paginas 359-382.

  3. Del arte común de curar a España y a las Indias occidentales

    Directory of Open Access Journals (Sweden)

    Estela Restrepo Zea

    1997-01-01

    Full Text Available Desde los primeros viajes colombinos, cronistas, soldados y otros viajeros llevaron hasta Europa noticias sobre las plantas americanas acompañadas de fabulosos relatos. Como en "Las palabras y las cosas", texto que para Foucault nació a partir del libro de Borges, de ese que cita "cierta enciclopedia china", así parecen haber entrado en el discurso de la historia natural, los seres y las cosas de las Indias occidentales desde el primer viaje de Colón. Seres que se volvieron "familiares en el Viejo Mundo, como a su edad y a su geografía". / incluye fragmento de un antidotario del siglo XVII en Cartagena de Indias. paginas 359-382.

  4. Analysis of toxicity of Anacardium occidentale L. extract submitted to ionizing radiation on embryos of Biomphalaria glabrata and Artemia salina

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Hianna A.M.F.; Sa, Jose L.F.; Lima, Claudia S.A.; Amancio, Francisco F.; Melo, Ana M.M.A., E-mail: hiannaamfs@gmail.com, E-mail: luismuma6@gmail.com, E-mail: claudia.salima@gmail.com, E-mail: amancioff@bol.com.br, E-mail: amdemelo@hotmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Departamento de Biofisica e Radiobiologia; Ribeiro, Luanna R.S.; Santos, Gustavo H.F.; Silva, Edvane B., E-mail: luannaribeiro_lua@hotmail.com, E-mail: santosghf@hotmail.com, E-mail: edvborges@yahoo.com.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Departamento de Energia Nuclear. Grupo de Estudos em Radioprotecao e Radioecologia

    2013-07-01

    The use of gamma radiation as a sterilization method for herbs, herbal medicines and foods, shows positive results regarding the retention of such products, economy and safety of the method. However, it is known that this method of processing plant material can cause chemical changes in these products related to the type of material, its components and the dose received. Evaluated, in the present study, the action of gamma radiation as a modifier of toxicity extract of Anacardium occidentale Linn. To evaluate the toxicity of the extract irradiated at doses of 5.0, 7.5 and 10.0 kGy and concentrations of 250, 500 and 1000 mg/L was used bioassays with Artemia salina and Biomphalaria glabrata. For the test to A. salina, 520 specimens were used divided into groups of 10 larvae. For the bioassay with B. glabrata, 3900 specimens were used divided into groups of, approximately, 100 embryos. Larvae of A. salina and embryos were subjected to extracts irradiated and unirradiated for 24 hours. The bioassay with A. salina, showed a decrease, compared to extract unirradiated and irradiated at doses of 5.0 and 7.5 kGy, of extract irradiated with 10 kGy, where the mortality did not differ from the control group. In tests with embryos was observed an increase in the toxicity of the extract at a dose of 7.5 kGy and a decrease in the dose of 10.0 kGy. The radiation promoted changes in the toxicity of leaves extracts of Anacardium occidentale Linn. on embryos of Biomphalaria glabrata and Artemia salina. (author)

  5. Molecular and phenotypic description of Stachybotrys chlorohalonata sp. nov and two chemotypes of Stachybotrys chartarum found in water-damaged buildings

    DEFF Research Database (Denmark)

    Andersen, Birgitte; Nielsen, Kristian Fog; Thrane, Ulf

    2003-01-01

    Twenty-five Stachybotrys isolates from two previous studies have been examined and compared, rising morphological, chemical and phylogenetic methods. The results show that S. chartarum sensu lato can be segregated into two chemotypes and one new species. The new species, S. chlorohalonata, differs...

  6. Chemical composition, olfactory analysis and antibacterial activity of Thymus vulgaris chemotypes geraniol, 4-thujanol/terpinen-4-ol, thymol and linalool cultivated in southern France.

    Science.gov (United States)

    Schmidt, Erich; Wanner, Jürgen; Hiiferl, Martina; Jirovetz, Leopold; Buchbauer, Gerhard; Gochev, Velizar; Girova, Tania; Stoyanova, Albena; Geissler, Margit

    2012-08-01

    The essential oils of four chemotypes of Thymus vulgaris L. (Lamiaceae) were analyzed for their composition and antibacterial activity to assess their different properties. GC-MS and GC-FID analyses revealed that the essentials oils can be classified into the chemotypes thymol (41.0% thymol), geraniol (26.4% geraniol), linalool (72.5% linalool) and 4-thujanol/terpinen-4-ol (42.2% cis- and 7.3% trans-sabinene hydrate, 6.5 % terpinen-4-ol). The olfactory examination confirmed the explicit differences between these chemotypes. Furthermore, antibacterial activity was investigated against several strains of two Gram-positive (Brochothrix thermosphacta and Staphylococcus aureus) and four Gram-negative food-borne bacteria (Escherichia coli, Salmonella abony, Pseudomonas aeruginosa and P. fragi). All essential oil samples were demonstrated to be highly effective against Gram-positive strains, whereas the impact on Gram-negative microorganisms was significantly smaller, but still considerable. The results obtained indicate that, despite their different properties, the essential oils of selected T. vulgaris chemotypes are potent antimicrobials to be employed as useful additives in food products as well as for therapeutic applications.

  7. Comparative inhibitory effect of prenylated coumarins, ferulenol and ferprenin, contained in the 'poisonous chemotype' of Ferula communis on mammal liver microsomal VKORC1 activity.

    Science.gov (United States)

    Louvet, Marie-Sophie; Gault, Gilbert; Lefebvre, Sébastien; Popowycz, Florence; Boulven, Manon; Besse, Stéphane; Benoit, Etienne; Lattard, Virginie; Grancher, Denis

    2015-10-01

    Two distinguishable chemotypes of Ferula communis have been described: the 'nonpoisonous' chemotype, containing as main constituents the daucane esters; and the 'poisonous' chemotype containing prenylated coumarins, such as ferulenol and ferprenin. Ferulenol and ferprenin are 4-oxygenated molecules such as dicoumarol and warfarin, the first developed antivitamin K molecules. Antivitamin K molecules specifically inhibit VKORC1, an enzyme essential for recycling vitamin K. This latest is involved in the activation of clotting factors II, VII, IX, X. The inhibiting effect of ferulenol on VKORC1 was shown in rat, but not for species exposed to F. communis while in vivo studies suggest differences between animal susceptibility to ferulenol. The inhibiting effect of ferprenin on VKORC1 was never demonstrated. The aim of this study was to compare the inhibiting effect of both compounds on VKORC1 of different species exposed to F. communis. Vitamin K epoxide activity was evaluated for each species from liver microsomes and inhibiting effect of ferulenol and ferprenin was characterized. Ferulenol and ferprenin were shown to be able to inhibit VKORC1 from all analyzed species. Nevertheless, susceptibility to ferulenol and ferprenin presented differences between species, suggesting a different susceptibility to 'poisonous' chemotypes of F. communis.

  8. A Comparison of Aggressiveness and Deoxynivalenol Production Between Canadian Fusarium graminearum Isolates with 3-Acetyl and 15-Acetyldeoxynivalenol Chemotypes in Field-Grown Spring Wheat

    Science.gov (United States)

    Twenty four isolates of Fusarium graminearum, half of which were 3- acetyldeoxynivalenol (3-ADON) and half 15-acetyldeoxynivalenol (15-ADON) chemotypes, were tested for their ability to produce deoxynivalenol and to cause Fusarium head blight (FHB), in spring wheat cultivars. The objectives of this...

  9. Citral and carvone chemotypes from the essential oils of Colombian Lippia alba (Mill. N.E. Brown: composition, cytotoxicity and antifungal activity

    Directory of Open Access Journals (Sweden)

    Ana Cecilia Mesa-Arango

    2009-09-01

    Full Text Available Two essential oils of Lippia alba (Mill. N.E. Brown (Verbenacea, the carvone and citral chemotypes and 15 of their compounds were evaluated to determine cytotoxicity and antifungal activity. Cytotoxicity assays for both the citral and carvone chemotypes were carried out with tetrazolium-dye, which showed a dose-dependent cytotoxic effect against HeLa cells. Interestingly, this effect on the evaluated cells (HeLa and the non-tumoural cell line, Vero was lower than that of commercial citral alone. Commercial citral showed the highest cytotoxic activity on HeLa cells. The antifungal activity was evaluated against Candida parapsilosis, Candida krusei, Aspergillus flavus and Aspergillus fumigatus strains following the standard protocols, Antifungal Susceptibility Testing Subcommittee of the European Committee on Antibiotic Susceptibility Testing and CLSI M38-A. Results demonstrated that the most active essential oil was the citral chemotype, with geometric means-minimal inhibitory concentration (GM-MIC values of 78.7 and 270.8 μg/mL for A. fumigatus and C. krusei, respectively. Commercial citral showed an antifungal activity similar to that of the citral chemotype (GM-MIC values of 62.5 μg/mL for A. fumigatus and 39.7 μg/mL for C. krusei. Although the citronellal and geraniol were found in lower concentrations in the citral chemotype, they had significant antifungal activity, with GM-MIC values of 49.6 μg/mL for C. krusei and 176.8 μg/mL for A. fumigatus.

  10. Citral and carvone chemotypes from the essential oils of Colombian Lippia alba (Mill.) N.E. Brown: composition, cytotoxicity and antifungal activity.

    Science.gov (United States)

    Mesa-Arango, Ana Cecilia; Montiel-Ramos, Jehidys; Zapata, Bibiana; Durán, Camilo; Betancur-Galvis, Liliana; Stashenko, Elena

    2009-09-01

    Two essential oils of Lippia alba (Mill.) N.E. Brown (Verbenacea), the carvone and citral chemotypes and 15 of their compounds were evaluated to determine cytotoxicity and antifungal activity. Cytotoxicity assays for both the citral and carvone chemotypes were carried out with tetrazolium-dye, which showed a dose-dependent cytotoxic effect against HeLa cells. Interestingly, this effect on the evaluated cells (HeLa and the non-tumoural cell line, Vero) was lower than that of commercial citral alone. Commercial citral showed the highest cytotoxic activity on HeLa cells. The antifungal activity was evaluated against Candida parapsilosis, Candida krusei, Aspergillus flavus and Aspergillus fumigatus strains following the standard protocols, Antifungal Susceptibility Testing Subcommittee of the European Committee on Antibiotic Susceptibility Testing and CLSI M38-A. Results demonstrated that the most active essential oil was the citral chemotype, with geometric means-minimal inhibitory concentration (GM-MIC) values of 78.7 and 270.8 microg/mL for A. fumigatus and C. krusei, respectively. Commercial citral showed an antifungal activity similar to that of the citral chemotype (GM-MIC values of 62.5 microg/mL for A. fumigatus and 39.7 microg/mL for C. krusei). Although the citronellal and geraniol were found in lower concentrations in the citral chemotype, they had significant antifungal activity, with GM-MIC values of 49.6 microg/mL for C. krusei and 176.8 microg/mL for A. fumigatus.

  11. La consommation de propylène en Europe occidentale Propylene Consumption in Western Europe

    Directory of Open Access Journals (Sweden)

    Barraqué M.

    2006-11-01

    Full Text Available Les besoins de l'Europe occidentale en propylène utilisé en pétrochimie sont actuellement de 7,4. 10 puissance 6 t/an. Au cours des prochaines années, le taux de progression moyen de la consommation devrait être de l'ordre de 1,8%/an; ainsi en 1995, la pétrochimie européenne utiliserait environ 8,7. 10 puissance 6 t de propylène. L'essentiel de la progression de cette consommation sera dû au fort accroissement de la production de polypropylène et à un degré moindre de l'oxyde de propylène, de l'isopropanol et de l'éthyl-2 hexanol. Par contre les débouchés représentés par l'acrylonitrile et le cumène resteront pratiquement stables. La consommation de propylène dans les productions de butanols devrait diminuer. La part des besoins en propylène satisfaite par les vapocraqueurs européens qui est en 1986 de 82 %, devrait être inférieure à 75 % en 1995. Il sera de plus en plus nécessaire de se tourner vers d'autres sources d'approvisionnement. En 1986 les raffineries européennes ont produit 1,05. 10 puissance 6 t de propylène utilisé en pétrochimie et les importations ont atteint 0,3. 10 puissance 6 t. En 1995 la différence entre la consommation et la production des vapocraqueurs pourrait dépasser 2,0. 10 puissance 6 t. Il semble peu probable que les unités de craquage catalytique puissent combler le déficit sauf si les rendements en propylène augmentent très notablement. On peut s'attendre à la construction de splitters propylène/propane et à une augmentation des importations. D'autre part, en raison de la tension qui risque d'apparaître sur les prix du propylène, l'intérêt de certaines voies de synthèse actuellement considérées comme non rentables pourrait augmenter; ces nouvelles voies sont : - la déshydrogénation du propane déjà considérée dans d'autres régions (Mexique, Indonésie, Malaisie; - la métathèse entre l'éthylène et les butènes-2, pouvant eux-mêmes être produits à partir d

  12. Effect of Niclosamide (Bayluscide WP 70 (R, Anacardium occidentale hexane extract and Euphorbia splendens latex on behavior of Biomphalaria glabrata (Say, 1818, under laboratory conditions

    Directory of Open Access Journals (Sweden)

    Pedro Jurberg

    1995-04-01

    Full Text Available The repellent effect of the molluscicides Niclosamide (Bayluscide WP 70 (R, Anacardium occidentale and the latex of Euphorbia splendens on Biomphalaria glabrata was observed through the investigation of the occurrence of escape behavior among molluscs that were exposed to dosages lower than the LD 50. The total number of individuals out of water among the surviving snails in the control group provided a "Natural Escape Index". The comparison between this total and the total number of surviving snails in each group exposed to the different dosages of the molluscicides after 24 hr provided the "Molluscicide Escape Index" and the detection of a "Repellency Range" to these snails. The escape indexes for Niclosamide, A. occidentale and E. splendens were 10, 6.22 and 6.44 respectively. Repellency occurred at the following concentration ranges: 0.01, 0.02 and 0.03 ppm Bayluscide, 0.1, 0.2 and 0.3 ppm A. occidentale and 0.05, 0.10, 0.15 and 0.20 ppm E. splendens. The Natural Escape Index obtained in the control group was zero.

  13. Pyrenean pastoralists’observations of environmental change: An exploratory study in los Valles Occidentales of Aragón

    Directory of Open Access Journals (Sweden)

    Fernández-Giménez, María E.

    2012-05-01

    Full Text Available Observations of environmental change by local resource users may be an important source of information about past and current environmental conditions to complement scientific studies and monitoring. In this exploratory, qualitative study, we documented observations of environmental change made by 27 stockmen in the two westernmost valleys of the Central Western Pyrenees of Spain. Pastoralists reported changes in weather, corresponding declines in the flow of mountain springs, and an increase in shrub and tree cover in the mountains. Explanations for the increase in woody plant cover differed in the two valleys; however, the majority of stockmen from both villages believed that the lack of human presence in the mountains contributes to shrub encroachment. Stockmen’s observations of environmental change suggest additional research needs regarding climate and vegetation change in the Pyrenees, and highlight the potential contributions of Pyrenean pastoralists’ local knowledge to environmental monitoring, research and management.Las observaciones sobre el cambio medioambiental realizadas por usuarios locales pueden ser una importante fuente de información sobre las características medioambientales del pasado y del presente para complementar los estudios científicos y el monitoreo. En este estudio cualitativo documentamos observaciones sobre el cambio medioambiental realizadas por 27 ganaderos en los dos valles más occidentales de los Pirineos centro occidentales españoles. Resumen cambios climáticos relativos a la disminución del caudal de las fuentes de montaña y al aumento de la cubierta vegetal de matorrales y arbolado en las montañas. Las explicaciones sobre el aumento de arbolado difieren en los dos valles; sin embargo la mayoría de los ganaderos de ambos pueblos pensaban que la falta de presencia humana en las montañas contribuye a la invasión del matorral. Las observaciones de los ganaderos sobre los cambios medioambientales

  14. [Development of mixed beverages made of caja (Spondias mombin L.) and cashew apple (Anacardium occidentale) added of fructooligosaccharides and inulin].

    Science.gov (United States)

    Silva, Larissa Morais Ribeiro da; Lima, Andréa da Silva; Maia, Geraldo Arraes; Rodrigues, Maria do Carmo Passos; Figueiredo, Raimundo Wilane de; Sousa, Paulo Henrique Machado de

    2011-06-01

    The purpose of this work was to develop three mixed drinks based on caja (Spondias mombin L.) and cashew apple (Anacardium occidentale) pulps, added prebiotic ingredients and to evaluate their chemical, physicochemical and sensory properties. Four formulations with combinations of two pulp fruit, sucrose and prebiotic ingredients (Standard inulin, inulin high performance-HP-and fructooligosaccharides FOS) were developed. The mixed drinks were submitted the following analysis pH, acidity, soluble solids, sugars, ascorbic acid, total carotenoids, total polyphenols and acceptance ratings of the sensory attributes such as: consistency, sweetness and overall impression, attitude and consumers purchase preference. The pH, total soluble solids and polyphenol results showed difference (p sweetness, consistency and attitude of buying showed no difference (p > 0.05) between drinks, however, differed significantly (p < or = 0.05) for the attribute of overall impression. The drink with FOS showed a similar acceptance when compared to traditional (sucrose) drink, showing an option of meeting the functional food expectations of consumers, who seek healthy, nutritious and tasty foods.

  15. Perlas y piel de azabache. El negro en las pesquerías de las Indias Occidentales

    Directory of Open Access Journals (Sweden)

    Tardieu, Jean Pierre

    2008-12-01

    Full Text Available Not very long after the Discovery, the pearls from the West Indies competed with those from the Orient for the full regalia of the ladies of the gentry, or that of the most revered virgins, while raising the curiosity of the best known chroniclers and creating an excruciating tax system. Considering the legislation passed for the benefit of Indians, those were to a large extent replaced in the “pearl fisheries” by black slaves submitted by the covetousness of their masters and the interests of the Crown to horrendous living and working conditions which most of the time relentlessly led them to death.

    Poco después del Descubrimiento, las perlas de las Indias occidentales rivalizaron con las de Oriente para el adorno de las damas de la alta sociedad o de las Vírgenes más veneradas, suscitando la curiosidad científica de los cronistas más conocidos y una exigente legislación fiscal. Debido a las leyes emitidas a favor de los indios, éstos se sustituyeron en gran parte, en las pesquerías de perlas, por esclavos negros sometidos —por la codicia de los amos y el interés de la Corona— a despiadadas condiciones de vida y de trabajo que desembocaban las más veces en una muerte inexorable.

  16. Leggere i Classici in Oriente. Il mito della letteratura occidentale in Dai Sijie, Murakami Haruki, Azar Nafisi

    Directory of Open Access Journals (Sweden)

    Niccolò Scaffai

    2011-11-01

    Full Text Available Il saggio prende in considerazione tre opere letterarie recenti (appartenenti tanto alla fiction quanto al genere del non-fiction novel, scritte da altrettanti autori di origini asiatiche: il franco-cinese Dai Sijie, l’iraniana Azar Nafisi e il giapponese Murakami Haruki. In ciascun titolo dei loro rispettivi bestseller internazionali (Balzac e la piccola sarta cinese, Leggere Lolita a Teheran, Kafka sulla spiaggia troviamo un ossimoro dello stesso tipo: il  nome di un autore occidentale canonico viene impiegato in un contesto insolito, legato alla geografia del vicino o dell’estremo Oriente. Ne consegue un effetto di straniamento che finisce per ridare forza alla percezione dell’opera letteraria, forse con maggiore decisione di quanto non sia possibile fare oggi in Occidente. Attraverso l’uso (e il rovesciamento del concetto di ‘orientalismo’ di Edward Said e, per Kafka sulla spiaggia, attraverso l’applicazione di principi della teoria di Lacan, lo studio analizza le dinamiche del trasferimento di miti letterari europei nelle culture orientali.

  17. Carotenoids, carotenoid esters, and anthocyanins of yellow-, orange-, and red-peeled cashew apples (Anacardium occidentale L.).

    Science.gov (United States)

    Schweiggert, Ralf M; Vargas, Ester; Conrad, Jürgen; Hempel, Judith; Gras, Claudia C; Ziegler, Jochen U; Mayer, Angelika; Jiménez, Víctor; Esquivel, Patricia; Carle, Reinhold

    2016-06-01

    Pigment profiles of yellow-, orange-, and red-peeled cashew (Anacardium occidentale L.) apples were investigated. Among 15 identified carotenoids and carotenoid esters, β-carotene, and β-cryptoxanthin palmitate were the most abundant in peels and pulp of all samples. Total carotenoid concentrations in the pulp of yellow- and red-peeled cashew apples were low (0.69-0.73 mg/100g FW) compared to that of orange-peeled samples (2.2mg/100g FW). The color difference between the equally carotenoid-rich yellow and red colored samples indicated the presence of a further non-carotenoid pigment type in red peels. Among four detected anthocyanins, the major anthocyanin was unambiguously identified as 7-O-methylcyanidin 3-O-β-D-galactopyranoside by NMR spectroscopy. Red and yellow peel color was chiefly determined by the presence and absence of anthocyanins, respectively, while the orange appearance of the peel was mainly caused by increased carotenoid concentrations. Thus, orange-peeled fruits represent a rich source of provitamin A (ca. 124 μg retinol-activity-equivalents/100g pulp, FW).

  18. Characterization and bioassay for larvicidal activity of Anacardium occidentale (cashew) shell waste fractions against dengue vector Aedes aegypti.

    Science.gov (United States)

    Torres, Rosalinda C; Garbo, Alicia G; Walde, Rikkamae Zinca Marie L

    2015-10-01

    Recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against Aedes aegypti larvae. Thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of Anacardium occidentale shell wastes against the third and fourth instar larvae of A. aegypti and to compare the results with the commercial larvicide product. The shell wastes were extracted with 95% EtOH followed by polarity-based fractionation. The fractions were tested for larvicidal activity according to the World Health Organization bioassay method. These were then characterized by quantitative thin-layer chromatographic (TLC) fingerprinting. The hexane fraction gave the strongest activity among the fractions with an LC50 of 4.01 mg/L and LC90 of 11.29 mg/L highly comparable to the commercial larvicide, which exhibited an LC50 of 1.71 mg/L and LC90 of 8.41 mg/L. The dichloromethane fraction exhibited 9.70 mg/L LC50 and 18.44 mg/L LC90. The remarkable toxicity effects exhibited by these fractions indicate their potential to provide core structures from which sustainable and environmentally safe plant-based larvicidal agents can be synthesized.

  19. Phenols and tannins contents of Anacardium occidentale Linn and Anadenanthera colubrina (Vell.) Brenan exposed to gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Gustavo Henrique Farias dos [Universidade Federal de Pernambuco (GERAR/UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Grupo de Estudos em Radioprotecao e Radioecologia; Silva, Edvane Borges da [Universidade Federal de Pernambuco (UFPE), Vitoria de Santo Antao, PE (Brazil). Centro Academico de Vitoria; Amorim, Elba Lucia Cavalcanti de; Peixoto Sobrinho, Tadeu, E-mail: elba@ufpe.b [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Ciencias Farmaceuticas; Lima, Claudia Sampaio de Andrade [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia

    2011-07-01

    Anacardium occidentale Linn (cajueiro) and Anadenanthera colubrina (Vell.) Brenan (angico) are very know as a source of phenolic compounds, mainly tannins. The aim of this study was to evaluate the difference of phenols and tannins contents of crude extracts were measured after irradiation of barks and leaves of each plant source, using a source of {sup 60}Co. The crude extracts were divided into control group and three groups which were separately after exposition to gamma radiation, in doses of 5; 7.5 and 10 kGy. From each group, the total phenols were quantified by the Folin-Ciocalteau method, while the contents of tannins were assessed using precipitation of the casein technique. For all doses, the total phenol and tannin percentages from 'cajueiro' barks presented no significant statistical alteration. However, for the leaves of 'cajueiro', their chemical composite levels significantly changed with the radiation absorbed dose. On the other hand, the gamma irradiation did not cause alterations in total phenols and tannins content of extracts from 'angico'. (author)

  20. Les cartes ethno-démographiques de l’Afrique occidentale: enjeux d’une construction 1952-1963

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    Marie-Albane de Suremain

    2008-12-01

    Full Text Available Entre 1952 et 1963, l’Institut français d’Afrique noire publie cinq cartes ethno-démographiques représentant l’Afrique occidentale de manière très synthétique au 1/1 000 000. Signe d’une démarche scientifique encore très positiviste et descriptive, elles représentent la distribution démographique et la répartition ethnique de la population. Les difficultés sont multiples: comment figurer des populations en déplacement, dont les identités se laissent mal enfermer dans des taxonomies ethniques et sans unité de mesure commune? Cet ensemble est significatif des évolutions de la cartographie coloniale, qui prolonge en Afrique des ambitions encyclopédiques révolues en métropole et s’intéresse à la catégorisation ethnique de la population, ce qui renvoie à des enjeux politiques. Mais les ethnologues, sociologues et géographes inventent aussi de nouvelles manières d’aborder territoires et populations en Afrique.

  1. Salmonella spp. e antibiotico-resistenza in Mammiferi e Uccelli selvatici in Italia nord-occidentale dal 2002 al 2010

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    Velca Botti

    2013-06-01

    Full Text Available La Salmonella è un importante agente patogeno responsabile di zoonosi di notevole rilevanza economica. In Europa la salmonellosi è la seconda infezione trasmessa per via alimentare, in Italia il microrganismo continua ad essere la causa più frequente di infezione alimentare. In Europa sono in atto molti piani di sorveglianza di Salmonella in animali da allevamento, tuttavia il monitoraggio del microrganismo in animali selvatici è effettuato solo occasionalmente. Lo studio ha avuto l'obiettivo di indagare la presenza di Salmonella in animali selvatici e i ceppi antibiotico-resistenti. Nel periodo 2002-2010, 2.713 animali selvatici (Canidi, Mustelidi, Uccelli, Roditori e Ungulati, provenienti da aree dell'Italia nord-occidentale, sono stati testati per Salmonella mediante metodo microbiologico colturale seguito da tipizzazione sierologica e biochimica. Di questi, 117 (63 Canidi, 25 Mustelidi, 24 Uccelli, 5 Ungulati sono risultati positivi per Salmonella (4,3%. Sono stati isolati 130 ceppi appartenenti a diversi sierotipi e Salmonella Typhimurium è risultato quello più rappresentato. La sensibilità agli antibiotici è stata testata su 88 ceppi con test di disco-diffusione. La maggior parte dei ceppi analizzati (97,7% si sono mostrati intermedi (I o resistenti (R ad almeno una classe di antibiotici. I più alti valori sono stati osservati per la classe delle tetracicline. La presenza di sierotipi di Salmonella antibiotico-resistenti e responsabili di zoonosi è stata riscontrata in diverse specie di animali selvatici.

  2. Characterization and functional analysis of eugenol O-methyltransferase gene reveal metabolite shifts, chemotype specific differential expression and developmental regulation in Ocimum tenuiflorum L.

    Science.gov (United States)

    Renu, Indu Kumari; Haque, Inamul; Kumar, Manish; Poddar, Raju; Bandopadhyay, Rajib; Rai, Amit; Mukhopadhyay, Kunal

    2014-03-01

    Eugenol-O-methyltransferase (EOMT) catalyzes the conversion of eugenol to methyleugenol in one of the final steps of phenylpropanoid pathway. There are no comprehensive reports on comparative EOMT gene expression and developmental stage specific accumulation of phenylpropenes in Ocimum tenuiflorum. Seven chemotypes, rich in eugenol and methyleugenol, were selected by assessment of volatile metabolites through multivariate data analysis. Isoeugenol accumulated in higher levels during juvenile stage (36.86 ng g(-1)), but reduced sharply during preflowering (8.04 ng g(-1)), flowering (2.29 ng g(-1)) and postflowering stages (0.17 ng g(-1)), whereas methyleugenol content gradually increased from juvenile (12.25 ng g(-1)) up to preflowering (16.35 ng g(-1)) and then decreased at flowering (7.13 ng g(-1)) and post flowering (5.95 ng g(-1)) from fresh tissue. Extreme variations of free intracellular and alkali hydrolysable cell wall released phenylpropanoid compounds were observed at different developmental stages. Analyses of EOMT genomic and cDNA sequences revealed a 843 bp open reading frame and the presence of a 90 bp intron. The translated proteins had eight catalytic domains, the major two being dimerisation superfamily and methyltransferase_2 superfamily. A validated 3D structure of EOMT protein was also determined. The chemotype Ot7 had a reduced reading frame that lacked both dimerisation domains and one of the two protein-kinase-phosphorylation sites; this was also reflected in reduced accumulation of methyleugenol compared to other chemotypes. EOMT transcripts showed enhanced expression in juvenile stage that increased further during preflowering but decreased at flowering and further at postflowering. The expression patterns may possibly be compared and correlated to the amounts of eugenol/isoeugenol and methyleugenol in different developmental stages of all chemotypes.

  3. Chemical Composition, and Antibacterial and Free-Radical-Scavenging Activities of the Essential Oils of a Citronellol Producing New Chemotype of Thymus pubescens Boiss. & Kotschy ex Celak

    Directory of Open Access Journals (Sweden)

    Satyajit D. Sarker

    2011-01-01

    Full Text Available The composition of the essential oils obtained from the flowering aerial parts of two populations of Thymus pubescens, collected from Mishov-Dagh, was determined by the GC-MS analyses. A total of 18 compounds, representing about 95% of the total oils, were identified in both samples of the essential oils. The essential oils of these two populations showed the presence of high amounts of citronellol (42.0% and 42.6%, geranyl acetate (14.0 and 14.0%, geraniol (13.0 and 13.1%, citronellyl acetate (3.9 and 3.8%, L-linalool (7.8% and 7.9%, cis-nerodiol (5.9% and 5.5% and citronellyl acetate (3.9% and 3.8%. However, in the published literature, carvacrol, thymol and p-cymene were reported to be the major compounds in T. pubescens. This significant difference in the composition of the essential oils was a clear evidence of chemical polymorphism with in the T. pubescens taxon, suggesting that these two populations of T. pubescens were in deed a new chemotype of this species, and the name Thymus pubescens Boiss. & Kotschy ex Celak chemotype Citronellol for this new chemotype has been proposed . The antibacterial and free-radical-scavenging properties of the essential oils of T. pubescens have also been evaluated.

  4. Atividade antimicrobiana do extrato de Anacardium occidentale Linn. em amostras multiresistentes de Staphylococcus aureus Antimicrobial activity of the hydroalcoholic extract of Anacardium occidentale Linn. against multi-drug resistant strains of Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Jackeline G. da Silva

    2007-12-01

    Full Text Available Plantas medicinais com propriedades terapêuticas são de grande relevância em todo o mundo, principalmente em países em desenvolvimento. A planta Anacardium occidentale Linn. é largamente usada na medicina tradicional na nossa região, como antidiarréico, para amigdalite, bronquites, artrites, e antiiflamatório. No presente estudo avaliou-se a ação antimicrobiana do extrato hidroalcóolico da casca do caule do cajueiro frente a amostras de Staphylococcus aureus resistentes e sensíveis à meticilina, obtidas a partir de pacientes internados do Hospital Universitário/Universidade Federal da Paraíba. A atividade antimicrobiana foi determinada pelo método de difusão em meio sólido para a determinação da Concentração Inibitória Mínima do extrato, e foi observada no total de 30 amostras, pela presença ou não do halo de inibição. Todas as amostras ensaiadas mostraram-se sensíveis à ação do extrato do cajueiro, com diâmetros dos halos de inibição variando de 10 a 20 mm, demonstrando grande eficácia do cajueiro. Assim, o uso desta planta na nossa região pode inferir uma alternativa terapêutica eficiente e de baixo custo, contra infecções bacterianas causadas por Staphylococcus aureus.Medicinal plants with therapeutical properties are of great significance in the whole world, especially in developing countries. Anacardium occidentale Linn. is a plant widely used in the traditional medicine in our region against diarrhea, tonsillitis, bronchitis, arthritis, and inflammation. In this paper, the antimicrobial activity of the hydroalcoholic extract of the cashew tree stem was evaluated against samples of meticillin-resistant and meticillin-sensible Staphylococcus aureus, attained from patients interned at Hospital Universitário/Universidade Federal da Paraíba. The antimicrobial activity was determined by the diffusion method in solid milieu to determine the Minimum Inhibitory Concentration (MIC of the extract, and it was

  5. Do co-occurring plant species adapt to one another? The response of Bromus erectus to the presence of different Thymus vulgaris chemotypes.

    Science.gov (United States)

    Ehlers, Bodil K; Thompson, John

    2004-11-01

    Local modification of the soil environment by individual plants may affect the performance and composition of associated plant species. The aromatic plant Thymus vulgaris has the potential to modify the soil through leaching of water-soluble compounds from leaves and litter decomposition. In southern France, six different thyme chemotypes can be distinguished based on the dominant monoterpene in the essential oil, which is either phenolic or non-phenolic in structure. We examine how soils from within and away from thyme patches in sites dominated by either phenolic or non-phenolic chemotypes affect germination, growth and reproduction of the associated grass species Bromus erectus. To do so, we collected seeds of B. erectus from three phenolic and three non-phenolic sites. Seeds and seedlings were grown on soils from these sites in a reciprocal transplant type experiment in the glasshouse. Brome of non-phenolic origin performed significantly better on its home soil than on soil from a different non-phenolic or a phenolic site. This response to local chemotypes was only observed on soil collected directly underneath thyme plants and not on soil in the same site (<5 m away) but where no thyme plants were present. This is preliminary evidence that brome plants show an adaptive response to soil modifications mediated by the local thyme chemotypes. Reproductive effort was consistently higher in brome of phenolic origin than in brome of non-phenolic origin (on both thyme- and grass-soil), indicating that life-history variation may be related to environmental factors which also contribute to the spatial differentiation of thyme chemotypes. Moreover, we found that brome growing on thyme-soil in general was heavier than when growing on grass-soil, regardless of the origin of the brome plants. This is concordant with thyme-soil containing higher amounts of organic matter and nitrogen than grass-soil. Our results indicate that patterns of genetic differentiation and local

  6. A 3D similarity method for scaffold hopping from known drugs or natural ligands to new chemotypes.

    Science.gov (United States)

    Jenkins, Jeremy L; Glick, Meir; Davies, John W

    2004-12-01

    A primary goal of 3D similarity searching is to find compounds with similar bioactivity to a reference ligand but with different chemotypes, i.e., "scaffold hopping". However, an adequate description of chemical structures in 3D conformational space is difficult due to the high-dimensionality of the problem. We present an automated method that simplifies flexible 3D chemical descriptions in which clustering techniques traditionally used in data mining are exploited to create "fuzzy" molecular representations called FEPOPS (feature point pharmacophores). The representations can be used for flexible 3D similarity searching given one or more active compounds without a priori knowledge of bioactive conformations or pharmacophores. We demonstrate that similarity searching with FEPOPS significantly enriches for actives taken from in-house high-throughput screening datasets and from MDDR activity classes COX-2, 5-HT3A, and HIV-RT, while also scaffold or ring-system hopping to new chemical frameworks. Further, inhibitors of target proteins (dopamine 2 and retinoic acid receptor) are recalled by FEPOPS by scaffold hopping from their associated endogenous ligands (dopamine and retinoic acid). Importantly, the method excels in comparison to commonly used 2D similarity methods (DAYLIGHT, MACCS, Pipeline Pilot fingerprints) and a commercial 3D method (Pharmacophore Distance Triplets) at finding novel scaffold classes given a single query molecule.

  7. Quantitative HPLC analysis of sesquiterpene lactones and determination of chemotypes in Eremanthus seidelii MacLeish and Schumacher (Asteraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Sakamoto, Humberto T. [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Filosofia, Ciencias e Letras. Dept. de Quimica; Gobbo-Neto, Leonardo; Lopes, Norberto P.; Lopes, Joao L.C. [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Ciencias Farmaceuticas. Dept. de Fisica e Quimica]. E-mail: joaoluis@usp.br; npelopes@fcfrp.usp.br; Cavalheiro, Alberto J. [UNESP, Araraquara, SP (Brazil). Inst. de Quimica

    2005-11-15

    anthus seidelii MacLeish and Schumacher has a restricted occurrence to the Brazilian 'cerrado' surrounding the Furnas (MG) reservoir, in environments that have been seriously damaged by human activity. The present phytochemical investigation reveals that the sesquiterpene lactones (SL) 4{beta},5-dihydro-2',3'-dihydroxy-15-desoxy-goyazensolide (1) and 4{beta},5-dihydro-1',2'-epoxy-eremantholide-C (2) are the major secondary metabolites in E. seidelii leaves, and an HPLC method was developed for their quantitative analysis. HPLC analysis showed no significant seasonal variation in the concentrations of both SL. No qualitative differences were found in the SL patterns of all individuals sampled. However, there is a different SL quantitative pattern among the plants analyzed, pointing to the existence of three quantitative chemotypes of this species, with differences possibly originating from the activity of the enzymes that cyclize the goyazensolide type SL (1) to a eremantholide type SL (2). (author)

  8. PROSPECÇÃO TECNOLÓGICA: APLICAÇÃO DA GOMA DO CAJUEIRO (Anacardium occidentale EM NANOTECNOLOGIA

    Directory of Open Access Journals (Sweden)

    celia cunha e silva

    2013-12-01

    Full Text Available O cajueiro (Anacardium occidentale, da família Anacardiaceae, é uma planta originária do Norte e Nordeste do Brasil, com arquitetura de copa tortuosa e de diferentes portes, bastante cultivada no Brasil, principalmente no Nordeste. O objetivo deste trabalho foi realizar uma prospecção, com busca de precedência e apresentar uma visão geral do estado atual de desenvolvimento científico e tecnológico relacionado à aplicação da goma do cajueiro na nonotecnologia, baseado no número de patentes depositadas e de artigos publicados. A prospecção foi realizada no Banco Europeu de Patentes (Europen Patent Office - EPO, no Banco da Organização Mundial de Propriedade Intelectual (World Intellectual Organization - WIPO, no Banco Americano de Marcas e Patentes (United States Patent and Trademark Office - USPTO e no Banco de dados do Instituto Nacional de Propriedade Industrial do Brasil – INPI, bem como na base de periódicos, Web of Science. O Brasil detém o maior número de artigos científicos envolvendo a Anacardium ocidentale, enquanto o Japão se sobressai na proteção desta pesquisa. Foi observado que das patentes depositadas nas referidas bases, mais da metade foram depositadas no período de 2000 a 2012, demostrando um aumento nas pesquisas relacionadas com o cajueiro nos últimos 12 anos, e que a maior parte das patentes possuem código de classificaçao de patente A61K e A61P que estão relacionadas a necessidades humanas.

  9. Innovations et métallurgies en Méditerranée occidentale (XIIIe–XVe siècles

    Directory of Open Access Journals (Sweden)

    Verna, Catherine

    2011-12-01

    Full Text Available After a definition of technical innovation in the Middle Ages and it’s relationship to industry, this paper explores it’s characteristics in the fi eld of iron and silver (silver-bearing lead and copper metallurgy from the 13th to the 15th centuries in the western Mediterranean area. The exploration opens with a synthesis of the circulation of iron and steel products, with special care taken over the definition of their respective qualities, in particular the distinction between iron and steel; the exploration continues with an introduction to specific technical territories related to industrial “districts”, which allows the author to present the study of the circulation of technical knowledge. The paper concludes with a study of notables (Catalan in particular as rural entrepreneurs who invested in the technical innovation of their time.[fr] Après avoir défi ni l’innovation technique au Moyen Âge et ses rapports à l’industrie, cet article en étudie les caractéristiques dans les domaines de la métallurgie du fer et de l’argent (plomb et cuivre argentifères du XIIIe au XVe siècle, en Méditerranée occidentale. Cet examen s’ouvre par un tableau de la circulation des produits sidérurgiques, attentif à la définition de leurs qualités respectives, en particulier à la distinction fer-acier; il se poursuit par la présentation des territoires techniques spécifiques associés à des districts industriels, ce qui permet à l’auteur d’aborder l’étude des circulations des savoirs techniques. L’article se conclut par une étude des notables (en particulier catalans, entrepreneurs ruraux, qui ont investi dans l’innovation technique.

  10. Harut et Marut, ou la présence de deux anges musulmans dans nombre de productions écrites occidentales

    OpenAIRE

    Arthur Chimkovitch

    2011-01-01

    À partir de la fin du XIIe siècle, le récit de Harut et Marut, anges musulmans coupables de fornication, apparaît dans certaines productions écrites occidentales. Cet article montre comment les premiers traducteurs du Coran, des théologiens, un pape, un empereur byzantin, des cosmographes, des historiens, des orientalistes, des encyclopédistes, des botanistes et finalement des écrivains français et anglais se sont servis de cet épisode, et l’ont véhiculé jusqu’au milieu du XIXe siècle.The sto...

  11. Hydroxylations of trichothecene rings in the biosynthesis of Fusarium trichothecenes: evolution of alternative pathways in the nivalenol chemotype.

    Science.gov (United States)

    Maeda, Kazuyuki; Tanaka, Akira; Sugiura, Ryosuke; Koshino, Hiroyuki; Tokai, Takeshi; Sato, Masayuki; Nakajima, Yuichi; Tanahashi, Yoshikazu; Kanamaru, Kyoko; Kobayashi, Tetsuo; Nishiuchi, Takumi; Fujimura, Makoto; Takahashi-Ando, Naoko; Kimura, Makoto

    2016-11-01

    Fusarium sporotrichioides genes FsTri11, FsTri13, and FsTri1 encode cytochrome P450 monooxygenases (CYPs) responsible for hydroxylations at C-15, C-4, and C-8 of the trichothecene skeleton, respectively. However, the corresponding genes of nivalenol (NIV)-chemotype Fusarium graminearum remain to be functionally elucidated. In this study, we characterized the roles of these CYPs in NIV biosynthesis. Analyses of the metabolites of the F. graminearum Fgtri11(-) mutant, a disruptant of FgTri11 encoding isotrichodermin (ITD) C-15 hydroxylase, revealed a small amount of NIV-type trichothecenes suggesting that an alternative C-15 hydroxylase partially complemented FgTRI11p. In contrast, the C-7/C-8 hydroxylations depended solely on FgTRI1p, as suggested by the metabolite profiles of the Fgtri11(-) Fgtri1(-) double gene disruptant. Disruption of FgTri1 in both the wild-type and Fgtri13(-) mutant backgrounds revealed that FgTRI13p exhibits marginal activity toward calonectrin (CAL) and that it was the only C-4 hydroxylase. In addition, feeding experiments demonstrated that the C-4 hydroxylation of a 7-hydroxytrichothecene lacking C-8 ketone was extremely limited. The marginal activity of FgTRI13p toward CAL was advantageous for the C-7/C-8 hydroxylation steps in NIV biosynthesis, as transformation of a C-4 oxygenated trichothecene lacking C-7/C-8 modifications into NIV-type trichothecenes was quite inefficient. The significance of hydroxylation steps in the evolution of Fusarium trichothecenes is discussed. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

  12. Inhibition of Hedgehog-dependent tumors and cancer stem cells by a newly identified naturally occurring chemotype

    Science.gov (United States)

    Infante, Paola; Alfonsi, Romina; Ingallina, Cinzia; Quaglio, Deborah; Ghirga, Francesca; D'Acquarica, Ilaria; Bernardi, Flavia; Di Magno, Laura; Canettieri, Gianluca; Screpanti, Isabella; Gulino, Alberto; Botta, Bruno; Mori, Mattia; Di Marcotullio, Lucia

    2016-01-01

    Hedgehog (Hh) inhibitors have emerged as valid tools in the treatment of a wide range of cancers. Indeed, aberrant activation of the Hh pathway occurring either by ligand-dependent or -independent mechanisms is a key driver in tumorigenesis. The smoothened (Smo) receptor is one of the main upstream transducers of the Hh signaling and is a validated target for the development of anticancer compounds, as underlined by the FDA-approved Smo antagonist Vismodegib (GDC-0449/Erivedge) for the treatment of basal cell carcinoma. However, Smo mutations that confer constitutive activity and drug resistance have emerged during treatment with Vismodegib. For this reason, the development of new effective Hh inhibitors represents a major challenge for cancer therapy. Natural products have always represented a unique source of lead structures in drug discovery, and in recent years have been used to modulate the Hh pathway at multiple levels. Here, starting from an in house library of natural compounds and their derivatives, we discovered novel chemotypes of Hh inhibitors by mean of virtual screening against the crystallographic structure of Smo. Hh functional based assay identified the chalcone derivative 12 as the most effective Hh inhibitor within the test set. The chalcone 12 binds the Smo receptor and promotes the displacement of Bodipy-Cyclopamine in both Smo WT and drug-resistant Smo mutant. Our molecule stands as a promising Smo antagonist able to specifically impair the growth of Hh-dependent tumor cells in vitro and in vivo and medulloblastoma stem-like cells and potentially overcome the associated drug resistance. PMID:27899820

  13. Diel cycles of isoprenoids in the emissions of Norway spruce, four Scots pine chemotypes, and in Boreal forest ambient air during HUMPPA-COPEC-2010

    Directory of Open Access Journals (Sweden)

    N. Yassaa

    2012-08-01

    Full Text Available Branch enclosure based emission rates of monoterpenes and sesquiterpenes from four Scots pines (Pinus sylvestris and one Norway spruce (Picea abies, as well as the ambient mixing ratios of monoterpenes were determined during the HUMPPA-COPEC 2010 summer campaign. Differences in chemical composition and in emission strength were observed between the different trees, which confirmed that they represented different chemotypes. The chemotypes of Scots pine can be classified according to species with high, no and intermediate content of Δ-3-carene. The "non-Δ-3-carene" chemotype was found to be the strongest emitter of monoterpenes. From this chemotype, β-myrcene, a very reactive monoterpene, was the dominant species accounting for more than 32 % of the total emission rates of isoprenoids followed by β-phellandrene (~27%. Myrcene fluxes ranged from 0.8 to 24 μg g−1 (dw h−1. α-Farnesene was the dominant sesquiterpene species, with average emission rates of 318 ng g−1 (dw h−1. In the high Δ-3-carene chemotype, more than 48% of the total monoterpene emission was Δ-3-carene. The average Δ-3-carene emission rate (from chemotype 3, circa 609 ng g−1 (dw h−1 reported here is consistent with the previously reported summer season value. Daily maximum temperatures varied between 20 and 35 °C during the measurements. The monoterpene emissions from spruce were dominated by limonene (35%, β-phellandrene (15%, α-pinene (14% and eucalyptol (9%. Total spruce monoterpene emissions ranged from 0.55 up to 12.2 μg g−1 (dw h−1. Overall the total terpene flux (monoterpenes + sesquiterpenes from all studied tree species varied from 230 ng g−1 (dw h−1 up to 66 μg g−1 (dw h−1. Total ambient monoterpenes (including α-pinene, Δ-3-carene, β-pinene and β-myrcene measured during the campaign

  14. 蓝翠雀花退化雄蕊上的黄色髯毛对其繁殖成功的影响%Effects of the yellow barbs of the staminodes on reproductive success of Delphinium caeruleum (Ranunculaceae)

    Institute of Scientific and Technical Information of China (English)

    张婵; 查绍琴; 杨永平; 段元文

    2012-01-01

    Nectar guides are special floral structures that are assumed to attract and/or direct pollinators towards a concealed nectar reward. Nectar guides are commonly found in many angiosperm plants, especially entomophilous flowers, but their functional significance and impacts on the reproductive success of plants are poorly understood. For example, Delphinium caeruleum of the family Ranunculaceae possesses two barbate staminodes, between which is the nectar entrance. To test the effects of these barbs on male and female fitness, we experimentally investigated the effects of barb removal on seed production and pollen exportation of D. caeruleum at the Haibei Station of Qinghai Province. Our results showed that flowers of D. caeruleum were dichogamous and herkogamous, which prevented autonomous selfing in this species. The mating system of D. caeruleum belongs to obligate xenogamy and thus pollinators are necessary for the successful pollination. Compared to control flowers, the number of pollen grains exported was reduced significantly after removing the barbs, but fruit set and seed number was not affected. Our primary results indicate that the barbs on the staminode act as nectar guides, which improve the male fitness of D. caeruleum, but have little effect on female fitness. Our results suggest that selection through male fitness might have played an impor- tant role in the floral evolution of D. caeruleum at our study site. These results need to be further investigated at a larger number of sites to evaluate selection mechanisms on floral traits of D. caeruleum.%蜜导是一类对传粉者具有特殊吸引和指示作用的花图案或花结构,在被子植物尤其是虫媒花中普遍存在,但目前有关蜜导对植物繁殖成功影响的研究相对较少.在野外条件下,我们以毛茛科翠雀属的蓝翠雀花(Delphinium caeruleum)为研究对象,通过人工授粉和去除髯毛实验对其交配系统和退化雄蕊上黄色髯毛的作用进行了

  15. Taxonomic Studies on the GenusDelphinium(Ranunculaceae) from China (V):Two New Synonyms ofD. autumnale%中国毛茛科翠雀属的分类学研究(五):秋翠雀花二新异名

    Institute of Scientific and Technical Information of China (English)

    袁琼; 杨亲二

    2015-01-01

    通过标本室和野外观察,发现根据四川西南部越西标本描述的毛茛科凉山翠雀花(Delphinium liangshanenseW. T. Wang)和根据四川西南部木里标本描述的光轴翠雀花(D. leiostachyumW. T. Wang)只是此前记载分布于四川木里、盐源和云南西北部宁蒗一带的秋翠雀花(D. autumnaleHand.-Mazz.)植株较高的类型,故将二者均处理为秋翠雀花的异名。%Delphinium liangshanenseW. T. Wang andD. leiostachyumW. T. Wang (Ranunculaceae) were described respectively from Yuexi and Muli in southwestern Sichuan, China. Both herbarium and ifeld observations have shown that they represent no more than a taller form ofD. autumnaleHand.-Mazz., a species previously recorded to occur in southwestern Sichuan (Muli, Yanyuan) and northwestern Yunnan (Ninglang). We therefore reduce them to the synonymy ofD. autumnale.

  16. Additive antimicrobial [corrected] effects of the active components of the essential oil of Thymus vulgaris--chemotype carvacrol.

    Science.gov (United States)

    Iten, Felix; Saller, Reinhard; Abel, Gudrun; Reichling, Jürgen

    2009-09-01

    Herbal remedies are multicomponent mixtures by their nature as well as by pharmaceutical definition. Being a multicomponent mixture is not only a crucial property of herbal remedies, it also represents a precondition for interactions such as synergism or antagonism. Until now, only a few phytomedicines are accurately described concerning the interactions of their active components. The aim of this study was to search for interactions within such a naturally given multi-component mixture and to discuss the pharmaceutical and clinical impacts. The thyme oil chosen for the examination belongs to the essential oils with the most pronounced antimicrobial activity. Antibiotic activity of thyme oil and single active components were tested against six different strains of microorganisms. The checkerboard assay was used to search for interactions. The time-kill assay was used to verify the observed effects and to get information about the temporal resolution of the antimicrobial activity. The degree of the detected interactions corresponded with the demarcating FICI measure of 0.5, which separates the additive from the over-additive (synergistic) effects. Therefore, the observed effect was called a "borderline case of synergism" or, respectively, "partial synergism". Partial synergism was observed only in the presence of Klebsiella pneumoniae. Additive antimicrobial activity was observed for the combination of the two monosubstances carvacrol plus linalool and thymol plus linalool as well as with the combination of the two essential oils of the carvacrol and linalool chemotypes. An increase of the carvacrol oil concentration from one to two times the MIC resulted in a considerable acceleration of the kill-rate. Thyme oil is composed of several different components that show antimicrobial activity (at least: carvacrol, thymol and linalool). The antimicrobial activity of thyme oil is partly based on additive effects, which might especially enhance the rapidity of the

  17. Central effects of citral, myrcene and limonene, constituents of essential oil chemotypes from Lippia alba (Mill.) n.e. Brown.

    Science.gov (United States)

    do Vale, T Gurgel; Furtado, E Couto; Santos, J G; Viana, G S B

    2002-12-01

    Citral, myrcene and limonene (100 and 200 mg/kg body wt., i.p.), constituents of essential oils from Lippia alba chemotypes, decreased not only the number of crossings but also numbers for rearing and grooming, as measured by the open-field test in mice. Although muscle relaxation detected by the rota rod test was seen only at the highest doses of citral (200 mg/kg body wt.) and myrcene (100 and 200 mg/kg body wt.), this effect was observed even at the lowest dose of limonene (50 mg/kg body wt.). Also, citral and myrcene (100 and 200 mg/kg body wt.) increased barbiturate sleeping time as compared to control. Limonene was also effective at the highest dose, and although citral did not increase the onset of sleep, it increased the duration of sleep, which is indicative of a potentiation of sleeping time. Citral (100 and 200 mg/kg body wt.) increased 2.3 and 3.5 times, respectively, the barbiturate sleeping time in mice. Similar effects were observed for myrcene and limonene at the highest dose (200 mg/kg body wt.) which increased the sleeping time around 2.6 times. In the elevated-plus maze, no effect was detected with citral up to 25 mg/kg body wt., while at a high dose it decreased by 46% the number of entries in the open arms. A smaller but significant effect was detected with limonene (5 mg/kg body wt.). While myrcene (10 mg/kg body wt.) decreased only by 22% the number of entries in the open arms, this parameter was decreased by 48% at the highest dose. Our study showed that citral, limonene and myrcene presented sedative as well as motor relaxant effects. Although only at the highest dose, they also produced a potentiation of the pentobarbital-induced sleeping time in mice, which was more intense in the presence of citral. In addition, neither of them showed an anxiolytic effect, but rather a slight anxiogenic type of effect at the higher doses.

  18. Randomized double blind trial of an extract from the pentacyclic alkaloid-chemotype of uncaria tomentosa for the treatment of rheumatoid arthritis.

    Science.gov (United States)

    Mur, Erich; Hartig, Frank; Eibl, Günther; Schirmer, Michael

    2002-04-01

    To evaluate safety and clinical efficacy of a plant extract from the pentacyclic chemotype of Uncaria tomentosa (UT) in patients with active rheumatoid arthritis (RA). Forty patients undergoing sulfasalazine or hydroxychloroquine treatment were enrolled in a randomized 52 week, 2 phase study. During the first phase (24 weeks, double blind, placebo controlled), patients were treated with UT extract or placebo. In the second phase (28 weeks) all patients received the plant extract. Twenty-four weeks of treatment with the UT extract resulted in a reduction of the number of painful joints compared to placebo (by 53.2% vs 24.1%; p = 0.044). Patients receiving the UT extract only during the second phase experienced a reduction in the number of painful (p = 0.003) and swollen joints (p = 0.007) and the Ritchie Index (p = 0.004) compared to the values after 24 weeks of placebo. Only minor side effects were observed. This small preliminary study demonstrates relative safety and modest benefit to the tender joint count of a highly purified extract from the pentacyclic chemotype of UT in patients with active RA taking sulfasalazine or hydroxychloroquine.

  19. Survey of foliar monoterpenes across the range of jack pine reveal three widespread chemotypes: implications to host expansion of invasive mountain pine beetle

    Directory of Open Access Journals (Sweden)

    Spencer eTaft

    2015-05-01

    Full Text Available The secondary compounds of pines (Pinus can strongly affect the physiology, ecology and behaviors of the bark beetles (Coleoptera: Curculionidae, Scolytinae that feed on sub-cortical tissues of hosts. Jack pine (Pinus banksiana has a wide natural distribution range in North America (Canada and USA and thus variations in its secondary compounds, particularly monoterpenes, could affect the host expansion of invasive mountain pine beetle (Dendroctonus ponderosae, which has recently expanded its range into the novel jack pine boreal forest. We investigated monoterpene composition of 601 jack pine trees from natural and provenance forest stands representing 63 populations from Alberta to the Atlantic coast. Throughout its range, jack pine exhibited three chemotypes characterized by high proportions of α-pinene, β-pinene, or limonene. The frequency with which the α-pinene and β-pinene chemotypes occurred at individual sites was correlated to climatic variables, such as continentality and mean annual precipitation, as were the individual α-pinene and β-pinene concentrations. However, other monoterpenes were generally not correlated to climatic variables or geographic distribution. Finally, while the enantiomeric ratios of β-pinene and limonene remained constant across jack pine’s distribution, (‒:(+-α-pinene exhibited two separate trends, thereby delineating two α-pinene phenotypes, both of which occurred across jack pine’s range. These significant variations in jack pine monoterpene composition may have cascading effects on the continued eastward spread and success of D. ponderosae in the Canadian boreal forest.

  20. (Anacardium occidentale) in Nigeria

    African Journals Online (AJOL)

    STORAGESEVER

    2008-12-29

    Dec 29, 2008 ... native of Tropical Central and South America, notably. North Eastern Brazil, from ..... wide acceptability in European and American markets. The Government should ... Active substances in cashew nut shell with molluscicidal ...

  1. Avaliação da toxicidade subcrônica do extrato bruto seco de Anacardium occidentale Linn em cães - DOI: 10.4025/actascihealthsci.v28i1.1112 Evaluation of the subchronic toxicity of the crude dry extract of Anacardium occidentale Linn in dogs - DOI: 10.4025/actascihealthsci.v28i1.1112

    Directory of Open Access Journals (Sweden)

    Isabely de Souza Vera Cruz

    2006-03-01

    Full Text Available A busca de novos medicamentos tem levado ao desenvolvimento de novos fármacos que sejam eficientes e destituídos de toxicidade. Uma das fronteiras nessas pesquisas são os medicamentos fitoterápicos. No Brasil, a Agência Nacional de Vigilância Sanitária (ANVISA regulariza essas pesquisas e padroniza os procedimentos. A Resolução da Diretoria Colegiada (RDC 48/2004, por exemplo, regulariza o registro de fitoterápicos. O Anacardium occidentale Linn está entre as plantas mais estudadas, devido às ações antibiótica e antiinflamatória de seus metabólitos secundários, principalmente taninos. Esta planta também possui a capacidade de impedir a formação da placa bacteriana bucal. Diante dessas ações, formas farmacêuticas acabadas (cremes e géis foram desenvolvidas a partir do extrato bruto seco (EBS das cascas do caule do A. occidentale Linn para registro de um novo fitomedicamento. Entretanto, testes pré-clínicos e clínicos devem ser feitos de acordo com a lei vigente. O presente trabalho avaliou a toxicidade subcrônica do EBS em cães sem raça definida (SRD. Os testes revelaram apenas hepatotoxicidade transitória demonstrada pela elevação dos níveis da alanina transaminase (ALT e aspartato transaminase (ASTResearch on new medicaments has led to the development of efficient and non-toxic drugs. In Brazil, the Agência Nacional de Vigilância Sanitária (National Department of Sanitary Supervision – ANVISA regularizes and standardizes the procedure. Anacardium occidentale is amongst the most researched plants, due to the antibiotics and antinflammatory properties of its secondary metabolites, mainly tannins and flavonoids. Furthermore, it prevents the dental plaque formation. On account of these actions, finished pharmaceutical forms (creams and gels were developed from the crude dry extract (CDE of A. occidentale Linn stem rinds, in order to register a new form. However, pre-clinical and clinical assays can be made in

  2. Contribution of the cashew gum (Anacardium occidentale L.) for development of layer-by-layer films with potential application in nanobiomedical devices.

    Science.gov (United States)

    Araújo, I M S; Zampa, M F; Moura, J B; dos Santos, J R; Eaton, P; Zucolotto, V; Veras, L M C; de Paula, R C M; Feitosa, J P A; Leite, J R S A; Eiras, C

    2012-08-01

    The search for bioactive molecules to be employed as recognition elements in biosensors has stimulated researchers to pore over the rich Brazilian biodiversity. In this sense, we introduce the use of natural cashew gum (Anacardium occidentale L.) as an active biomaterial to be used in the form of layer-by-layer films, in conjunction with phthalocyanines, which were tested as electrochemical sensors for dopamine detection. We investigated the effects of chemical composition of cashew gum from two different regions of Brazil (Piauí and Ceará states) on the physico-chemical characteristics of these nanostructures. The morphology of the nanostructures containing cashew gum was studied by atomic force microscopy which indicates that smooth films punctuated by globular features were formed that showed low roughness values. The results indicate that, independent of the origin, cashew gum stands out as an excellent film forming material with potential application in nanobiomedical devices as electrochemical sensors.

  3. M. Segard, Les Alpes occidentales romaines. Développement urbain et exploitation des ressources des régions de montagne

    Directory of Open Access Journals (Sweden)

    Alain Ferdière

    2010-02-01

    Full Text Available Comme l’indique le texte de présentation (en p. 4 de couverture – comme d’ailleurs la préface de Philippe Leveau (p. 9-10 –, le propos de la Thèse de Doctorat de Maxence Segard (Université de Provence, ss la dir. de Ph. Leveau, 2005, ici heureusement publiée, est de réviser en profondeur la vision classique des Alpes – pour la partie occidentale de l’arc –, telle que vue à travers le prisme du topos romain, grâce aux données certes aussi textuelles et épigraphiques, mais surtout archéologi...

  4. Essential-oil variability of Juniperus deltoides R.P.Adams along the east Adriatic coast - how many chemotypes are there?

    Science.gov (United States)

    Rajčević, Nemanja; Janaćković, Peđa; Dodoš, Tanja; Tešević, Vele; Marin, Petar D

    2015-01-01

    The composition of the essential oils isolated from twigs of ten Juniperus deltoides R.P. Adams populations from the east Adriatic coast was determined by GC-FID and GC/MS analyses. Altogether, 169 compounds were identified, representing 95.6-98.4% of the total oil composition. The oils were dominated by monoterpenes (average content of 61.6%), which are characteristic oil components of species of the Juniperus section. Two monoterpenes, α-pinene and limonene, were the dominant constituents, comprising on average 46.78% of the essential oils. Statistical methods were deployed to determine the diversity of the terpene classes and the common terpenes between the investigated populations. These statistical analyses revealed the existence of three chemotypes within all populations, i.e., a α-pinene, limonene, and limonene/α-pinene type. Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich.

  5. 皱叶薄荷精油的化学分类特征%Analysis on Chemotype of Volatile Oil of Mentha crispata Schrad. ex Willd

    Institute of Scientific and Technical Information of China (English)

    郭晓恒; 刘涛; 宋登敏; 雨田; 严铸云

    2014-01-01

    This study was aimed to analyze the volatile oil of Mentha crispata Schrad. ex Willd. in order to provide evidence for its chemotype and guidance for its production application. The chemical analysis was detected by headspace GC-MS. The results showed that 64 chemical compounds were detected. It was concluded that the volatile oil of M. crispata Schrad. ex Willd. mainly contained eucalyptol (35.58%), limonene (16.92%) and pinene (15.33%). It was concluded that the analysis on composition characteristics and main compounds of M. crispata Schrad. ex Willd. can provide evidences in its production application and chemotype.%目的:对皱叶薄荷精油进行成分分析,为种源鉴定提供化学分类依据并为生产应用提供指导。方法:采用顶空气相法对皱叶薄荷全草进行化学分析。结果:共得到64个化学成分,且皱叶薄荷主要由桉油素(Eucalyptol ,35.58%)、柠檬烯(Limonene ,16.92%)及蒎烯(Pinene ,15.33%)组成。结论:通过分析皱叶薄荷精油的组成特征及主要成分,可为其生产应用及种源鉴定提供化学分类佐证。

  6. Neuro-protective potential of a vesicular system of a standardized extract of a new chemotype of Withania somnifera Dunal (NMITLI118RT+) against cerebral stroke in rats().

    Science.gov (United States)

    Ahmad, Hafsa; Khandelwal, Kiran; Samuel, Sheeba Saji; Tripathi, Shivangi; Mitra, Kalyan; Sangwan, Rajender Singh; Shukla, Rakesh; Dwivedi, Anil Kumar

    2016-09-01

    Withania somnifera Dunal is an Indian medicinal plant with significant pharmacological properties, such as adaptogenic, anti-inflammatory, anti-oxidant, anti-platelet, anti-hypertensive, hypoglycemic and hypolipidemic effects. Several chemotypes of W. somnifera include NMITLI-101, NMITLI-118 and NMITLI-128. The present work elaborates the optimization and development of a liposomal delivery system for efficient delivery of NMITLI118RT+ [a standardized ethanolic extract of a new chemotype of W. somnifera Dunal (NMITLI-118) roots] against cerebral stroke in rats. Liposomal systems were prepared using thin-film hydration method and characterized on the basis of size, zeta potential, physical stability, FT-IR, DSC-TGA analysis and surface morphological studies by TEM. NMITLI118RT+ and its formulations (NMITLI118RT+LF) were evaluated for biological activity utilizing middle cerebral artery occlusion model in rats. The Z average of the developed liposomal formulation was about 142.6 ± 0.09 nm with a zeta potential of -31.20 ± 1.0 mV. Results of TEM revealed spherical particles in the range of 200 nm. The entrapment efficiency was found to be 94.603 ± 2%. The formulation was found to be physically stable over a 3-week period. Results were suggestive of the fact that both NMITLI118RT+ and its delivery system possess significant neuroprotective activity in cerebral ischemia. The liposomal system largely exhibits better performance over NMITLI118RT+ precisely in the post-treatment group. The present studies could elucidate the successful development of a delivery system for NMITLI118RT+ and demonstrate their beneficial neuro-protective potential in overcoming and reversing the consequences of I/R injury following stroke.

  7. Circulation et modélisation de la Méditerranée Occidentale et du Golfe du Lion - Synthèse des connaisssances et des travaux existants

    OpenAIRE

    Obaton, Dominique

    1998-01-01

    Ce travail est un état des connaissances de la circulation et de la modélisation numérique de la Méditerranée occidentale. Il s'attache à décrire les études existantes en Méditerranée occidentale dans son ensemble, soit la zone comprise entre les détroits de Gibraltar et de Sicile, et celles du golfe du Lion qui ont fait l'objet d'une littérature spécifique. Autant que possible, les résultats obtenus par la modélisation sont comparés aux mêmes observations et mesures in situ ainsi que les uns...

  8. Antimutagenic activity of cashew apple (Anacardium occidentale Sapindales, Anacardiaceae) fresh juice and processed juice (cajuína) against methyl methanesulfonate, 4-nitroquinoline N-oxide and benzo[a]pyrene

    OpenAIRE

    2008-01-01

    Cashew apple juice (CAJ), produced from the native Brazilian cashew tree (Anacardium occidentale), and has been reported to have antibacterial, antifungal, antitumor, antioxidant and antimutagenic properties. Both the fresh unprocessed juice and the processed juice (cajuína in Portuguese) has been shown to consist of a complex mixture containing high concentrations of anacardic and ascorbic acids plus several carotenoids, phenolic compounds and metals. We assessed both types of juice for thei...

  9. Extracción y Caracterización Fisicoquímica del Aceite de la Semilla (Almendra del Marañón (Anacardium occidentale L Extraction and Physicochemical Characterization of Seed Oil (Almond of Cashew nut (Anacardium occidentale L

    Directory of Open Access Journals (Sweden)

    Jennifer J Lafont

    2011-01-01

    Full Text Available El objetivo principal del trabajo que se presenta en este artículo es la evaluación de diferentes métodos de extracción de aceite de semillas de almendras (Anacardium occidentale L y caracterización de sus propiedades físico-químicas. El procedimiento consiste en someter la muestra a procesos de extracción mecánica o prensado y a extracción con solventes aplicando dos métodos: inmersión e inmersión-percolación. El método más eficiente fue el de inmersión-percolación usando n-hexano como solvente. El rendimiento obtenido es del (97.78 ± 1.32%. El análisis de ácidos grasos por HPLC reveló un alto contenido de ácido oleico (61.36%. De estos resultados se recomienda la extracción con solventes para la industria de jabones, cosméticos y la extracción con prensado para la industria alimenticia.The main objective of the work presented in this paper is the evaluation of different methods for the extraction of almond oil (Anacardium occidentale L and the characterization of its physicochemical properties. The procedure involves subjecting the sample to mechanical extraction process, or pressing and solvent extraction using two methods, immersion and immersion-percolation. The most efficient method was the immersion-percolation using n-hexane as solvent. The yield obtained is (97.78 ± 1.12%. The fatty acid analysis by HPLC revealed high contení of oleic acid (61.36%. From these results solvent extraction is recommended for the soap industry, for cosmetics and extraction by pressing in the food industry.

  10. Effects of immature cashew nut-shell liquid (Anacardium occidentale) against oxidative damage in Saccharomyces cerevisiae and inhibition of acetylcholinesterase activity.

    Science.gov (United States)

    De Lima, S G; Feitosa, C M; Citó, A M G L; Moita Neto, J M; Lopes, J A D; Leite, A S; Brito, M C; Dantas, S M M; Cavalcante, A A C Melo

    2008-09-09

    The cashew tree (Anacardium occidentale) represents one of the major cheapest sources of non-isoprenoid phenolic lipids, which have a variety of biological properties: they can act as molluscicides, insecticides, fungicides, have anti-termite properties, have medicinal applications, and demonstrate antioxidant activity in vitro. Immature cashew nut-shell liquid (iCNSL) is a unique natural source of unsaturated long-chain phenols. Their use has stimulated much research in order to prepare drug analogues for application in several fields. The objective of the present study was to determine whether iCNSL has antioxidant properties when used in strains of the yeast Saccharomyces cerevisiae and to measure the inhibitory activity of acetylcholinesterase. The constituents were identified using thin-layer chromatography, gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy, and (1)H and (13)C nuclear magnetic resonance. The iCNSL contains anacardic acid, cardanol, cardol, and 2-methyl cardol. Immature cashew nut oil contains triacylglycerols, fatty acids, alkyl-substituted phenols, and cholesterol. The main constituents of the free fatty acids are palmitic (C(16:0)) and oleic acid (C(18:1)). iCNSL has excellent protective activities in strains of S. cerevisiae against oxidative damage induced by hydrogen peroxide and inhibits acetylcholinesterase activity. iCNSL may have an important role in protecting DNA against damage induced by reactive oxygen species, as well as hydrogen peroxide, generated by intra- and extracellular mechanisms.

  11. Géochimie des gaz naturels et origine des gisements de gaz en Europe occidentale Geochemistry of Natural Gas and Origins of Gas Fields in Western Europe

    Directory of Open Access Journals (Sweden)

    Tissot B.

    2006-11-01

    Full Text Available De nombreux gisements de gaz sont connus en Europe, dans des conditions géologiques très diverses et dans des terrains d'âge Paléozoïque à Pleistocène. Le problème de leur origine peut être élucidé par l'étude de la composition chimique et isotopique des gaz associée à la connaissance de l'histoire géologique. On rappellera d'abord le schéma général de formation de l'huile et du gaz dans les bassins sédimentaires, puis on discutera les conditions particulières de formation des divers types de gaz. Enfin, on passera en revue, à titre d'exemple, un certain nombre de gisements de gaz d'Europe occidentale. A great many gas fields are known in Europe, having a wide variety of geological conditions and in formations ranging in age from the Paleozoic to the Pleistocene. The problem of their origin can be explained by analyzing the chemical and isotopic composition of the gases combined with knowing the geological history. This article first reviews the general pattern of oil and gas formation in sedimentary basins and then goes on to discuss the specific conditions for the formation of different types of gas. It concludes with a review of various gas fields in Western Europe.

  12. Deformación cuaternaria asociada al frente de levantamiento oriental de las sierras de Velasco y Ambato, Sierras Pampeanas occidentales

    Directory of Open Access Journals (Sweden)

    Analía L. Casa

    2010-12-01

    Full Text Available Sobre el frente oriental de la sierra de Velasco y extremo sur de la sierra de Ambato, Sierras Pampeanas Occidentales, se han hallado nuevas evidencias de actividad tectónica cuaternaria en cercanías de la ciudad de La Rioja. Los rasgos reconocidos corresponden a distintos tramos reactivados de las fallas que delimitan los frentes serranos y a estructuras que afectan los depósitos cuaternarios sobre el piedemonte. la sierra de Velasco Oriental se encuentra marginada por fallas de rumbo NNE e inclinación al ONO, mientras que la sierra de Ambato austral presenta estructuras de rumbo NE que inclinan al NO. En el bloque Carrizal estas direcciones se interfieren originando un sector de mayor complejidad estructural. La deformación observada evidencia la persistencia de un régimen compresivo durante el Cuaternario al que se asocian fallas inversas de vergencia oriental a sudoriental. Estas fallas continúan elevando los cordones serranos y constituyen potenciales fuentes sismogénicas.

  13. Rediscovery of Bouteloua vaneedenii (Gramineae: Chloridoideae: endemic species from the West Indies Redescubrimiento de Bouteloua vaneedenii (Gramineae: Chloridoideae: especie endémica de las Indias Occidentales

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    María Elena Siqueiros-Delgado

    2011-12-01

    Full Text Available Bouteloua vaneedenii is an endemic and extremely rare grass of the West Indies. Very few collections are known, and the most recent collection is from 1922. With the aim of finding the species, a team of Mexican and Cuban agrostologists conducted a field trip and found B. vaneedenii in the same locality where it was collected in 1922 on dry limestone rocks. Although it was stated that B. vaneedenii probably was extinct from Cuba, vigorous populations remain in at least 2 localities in Pastelillo. Further exploration may lead to the discovery of additional populations and the reevaluation of its current conservation status.Bouteloua vaneedenii es un pasto extremadamente raro, nativo de Las Indias Occidentales y del que muy pocas recolectas se conocen hasta ahora, la última se realizó en 1922. Con el objetivo de encontrar la especie, un equipo de agrostólogos mexicanos y cubanos condujeron un viaje de campo en busca de B. vaneedenii , la cual fue hallada en la misma localidad donde se recolectó sobre rocas calizas en 1922, a pesar de que se había señalado como probable extinta para Cuba. Se ubicaron 2 poblaciones en buenas condiciones; no obstante, es necesaria una exploración más intensa confirmar su estado actual de conservación.

  14. Contribution of the cashew gum (Anacardium occidentale L.) for development of layer-by-layer films with potential application in nanobiomedical devices

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, I.M.S. [Departamento de Quimica, Centro de Ciencias da Natureza, CCN, Universidade Federal do Piaui, UFPI, Teresina, PI, 64049-550 (Brazil); Nucleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Parnaiba, CMRV, Universidade Federal do Piaui, UFPI, Parnaiba, PI, 64202-020 (Brazil); Zampa, M.F. [Nucleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Parnaiba, CMRV, Universidade Federal do Piaui, UFPI, Parnaiba, PI, 64202-020 (Brazil); Campus Parnaiba, Instituto Federal de Educacao Ciencia e Tecnologia do Piaui, IFPI, Parnaiba, PI, 64210-260 (Brazil); Moura, J.B.; Santos, J.R. dos [Departamento de Quimica, Centro de Ciencias da Natureza, CCN, Universidade Federal do Piaui, UFPI, Teresina, PI, 64049-550 (Brazil); Eaton, P. [REQUIMTE, Faculdade de Ciencias da Universidade do Porto, Rua do Campo Alegre, Porto, 4169-007 (Portugal); Zucolotto, V. [Grupo de Biofisica Molecular Sergio Mascarenhas, Instituto de Fisica de Sao Carlos, IFSC, USP, Sao Carlos, SP, 13566-590 (Brazil); and others

    2012-08-01

    The search for bioactive molecules to be employed as recognition elements in biosensors has stimulated researchers to pore over the rich Brazilian biodiversity. In this sense, we introduce the use of natural cashew gum (Anacardium occidentale L.) as an active biomaterial to be used in the form of layer-by-layer films, in conjunction with phthalocyanines, which were tested as electrochemical sensors for dopamine detection. We investigated the effects of chemical composition of cashew gum from two different regions of Brazil (Piaui and Ceara states) on the physico-chemical characteristics of these nanostructures. The morphology of the nanostructures containing cashew gum was studied by atomic force microscopy which indicates that smooth films punctuated by globular features were formed that showed low roughness values. The results indicate that, independent of the origin, cashew gum stands out as an excellent film forming material with potential application in nanobiomedical devices as electrochemical sensors. Highlights: Black-Right-Pointing-Pointer This study focused on the use of cashew gum for the formation of LbL films. Black-Right-Pointing-Pointer LbL films containing cashew gums were investigated by AFM and cyclic voltammetry. Black-Right-Pointing-Pointer Cashew gum contributed to obtain stable films with well-defined redox processes. Black-Right-Pointing-Pointer Cashew gum films detected dopamine in low concentrations. Black-Right-Pointing-Pointer These LbL films presented potential application in nanobiomedical devices.

  15. Geología y vulcanología de La Palma y El Hierro, Canarias Occidentales

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    Pérez Torrado, F. J.

    2001-12-01

    construction of the El Golfo volcano seems to have taken place after a relatively long period of activity, probably coinciding with the maximum development of the Cumbre Nueva rift on La Palma. The initial subaerial activity at El Golfo was characterised by basaltic lavas that evolved to trachybasalts and trachytes, and finally towards more differentiated eruptive episodes indicative of the terminal state of the volcanic activity of the El Golfo volcano. The excessive growth of this volcano triggered the failure of its north flank, generating the spectacular scarp and present El Golfo depression. Subsequent volcanism, from emission vents arranged in a three-armed rift system (rift volcanism, with ages ranging from 145 ka to 2,500 years, with probably prehistoric eruptions, implies the much more moderate continuation of the earlier predominantly basanitic-tephritic volcanic activity. This period may correspond to that of maximum development of the Cumbre Vieja rift, in the island of La Palma.Las Canarias occidentales, relativamente poco estudiadas hasta hace unos años desde el punto de vista geológico, han aportado sin embargo datos decisivos para la comprensión de muchos de los problemas geológicos más importantes del archipiélago, que posiblemente se hubieran dilucidado más prontamente si su estudio se hubiese comenzado, como en la mayoría de las cadenas de islas volcánicas oceánicas, por su extremo más reciente. Como resumen de sus principales rasgos geológicos evolutivos de ambas islas destacamos las siguientes etapas de desarrollo: Durante el Plioceno se levanta en el extremo occidental del Archipiélago, en la isla de La Palma, un edificio o monte submarino constituido por pillow lavas, pillow brechas e hialoclastitas de composición basáltica, intruido por domos traquíticos, plutones de gabros y una densísima red de diques. Por el efecto de la intensa intrusión magmática y filoniana el edificio submarino sufrió un levantamiento hasta cotas de 1.500 m y

  16. La Coopération Scientifique et Technologique en Europe occidentale. 1ère Partie Scientific and Technological Cooperation in Western Europe. Part One

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    Bertrand V.

    2006-11-01

    Full Text Available L'exemple de la puissance américaine pendant et après le dernier conflit mondial a montré aux nations de l'Europe occidentale que leur développement économique dépendait étroitement de leur aptitude à la recherche scientifique et à l'innovation technologique. Devant la nécessité vitale d'élaborer une politique de la science et l'impossibilité de concurrencer individuellement les États-Unis sur l'ensemble du front, elles se sont progressivement engagées vers une collaboration de plus en plus réfléchie dans le domaine scientifique et technologique. Dans le même temps les grands organismes internationaux à vocation politique, économique ou militaire (ONU, OCDE, OTAN... créés après la guerre ont été aussi amenés à se préoccuper des problèmes de la science avant que ceux ci ne fassent l'objet d'une politique délibérée de coopération sous l'égide des Communautés européennes.Nous allons essayer. de faire le point sur toutes ces questions en exposant dans les différents chapitres I. La notion de politique de la science et son évolution dans le monde occidental. II. Les organismes internationaux impliqués dans une coopération scientifique et technologique en Europe occidentale. III et IV. Les résultats (scientifiques puis technologiques de cette coopération en dehors des grands organismes internationaux. V. Les actions menées dans le cadre de ces organismes. VI. La politique de coopération entreprise au sein des Communautés européennes. VII. La coopération européenne en matière d'énergie. The exemple of U.S. power during and after the last World War showed the countries in Western Europe that their economic development was closely linked to their capocity for scientific research and technological innovation. Faced with the vital need to draw up a science policy and the impossibilty of individually rivaling the United States in all areas, they gradually moved toward more and more deliberate coopération in

  17. Le système des intendances dans les Indes Occidentales : son impact sur le Trésor Royal

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    Manuela Domínguez-Orta

    2012-01-01

    Full Text Available Pendant la seconde moitié du XVIIIe siècle, l’une des réformes entreprises par la monarchie espagnole dans les Indes Occidentales fut l’établissement d’un système d’intendances. L’objectif de ce travail est d’analyser l’influence des intendances dans les finances royales, en s’intéressant au rôle qu’elles devaient jouer en matière de contrôle financier. Notre recherche porte sur la vice-royauté du Río de la Plata, car c’est là que les intendances furent établies avec succès avant d’être étendues au reste des territoires espagnols de l’Amérique.El presente estudio se contextualiza en la segunda mitad del siglo XVIII en las Indias Occidentales. Durante este período una de las reformas que llevó a cabo la Monarquía española en sus posesiones ultramarinas fue el establecimiento del sistema de intendencias. El objetivo de este trabajo es analizar la influencia de las intendencias en la Hacienda Real a través de aspectos contables. Como caso de estudio se ha tomado el Virreinato del Río de la Plata por haber sido el Virreinato en el que las intendencias se establecieron con éxito y desde donde se extendieron al resto de los territorios americanos.Der Artikel interessiert sich für den Verwaltungsaufbau der Intendanten, der zielt, ab 1700 den Kontrolle des spanischen Reiches zu verbessern, um die königlichen Einnahmen zu erhöhen. Der französische Einfluß hat in dieser Entstehung der Intendanten gelastet. Tatsächlich findet man, daß ihre Einführung auf der Iberischen Halbinsel während erster Hälfte des XVIII. Jahrhunderts von Jean Orry und ihren Mitarbeitern das Verdienst ist. Später sind die Intendanten in spanischem Amerika angesiedelt gewesen, und man rechtfertigte diese Verbreitung seit der Metropole von den Vorteilen, daß “die königlichen Finanzen, in der besten Verwaltung der Einnahmen erhalten hatten”. Bis hierher sind die Intendanten von unterschiedlichen Seiten studiert gewesen

  18. Harut et Marut, ou la présence de deux anges musulmans dans nombre de productions écrites occidentales

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    Arthur Chimkovitch

    2009-07-01

    Full Text Available À partir de la fin du XIIe siècle, le récit de Harut et Marut, anges musulmans coupables de fornication, apparaît dans certaines productions écrites occidentales. Cet article montre comment les premiers traducteurs du Coran, des théologiens, un pape, un empereur byzantin, des cosmographes, des historiens, des orientalistes, des encyclopédistes, des botanistes et finalement des écrivains français et anglais se sont servis de cet épisode, et l’ont véhiculé jusqu’au milieu du XIXe siècle.The story of Harut and Marut, two Muslim angels guilty of fornication, appeared in the Western written production at the end of the 12th century. This article shows how the episode was exploited by the first translators of the Coran, by theologians, by a pope, by a Byzantine emperor, by cosmographers, by historians, orientalists, encyclopedists, botanists and finally by some French and English writers, and how it was thus transmitted until the middle of the 19th century.Desde finales del siglo XII, la historia de Harut y Marut, ángeles musulmanes culpables de fornicación, aparece en ciertas producciones escritas en Occidente. Este artículo muestra cómo los primeros traductores del Corán, teólogos, un papa, un emperador bizantino, cosmógrafos, historiadores, orientalistas, enciclopedistas, botánicos, así como escritores franceses e ingleses, se sirvieron de este episodio, logrando que se transmitiera hasta mediados del siglo XIX.

  19. La Oscurana (Cajamarca, un bosque relicto más para conservar en las vertientes occidentales andinas del norte del Perú

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    Ana M. Juárez

    2013-05-01

    Full Text Available Se presenta un análisis de la composición florística, formas de vida, importancia y estrategias para la conservación de un bosque relicto de la Vertiente Occidental Andina del norte del Perú, descrito por primera vez y denominado La Oscurana (caserío El Nogal, distrito Bolívar, provincia San Miguel de Pallaques, Cajamarca; 6°90’S y 79°08’O, 2000-2800 m. El análisis preliminar incluye 85 familias, 169 géneros y 258 especies de plantas vasculares, de las cuales 15 familias corresponden a Pteridophyta y 70 a Magnoliophyta. En los ámbitos genérico y específico las Asteraceae son las mejor representadas de las Magnoliopsida y las Orchidaceae de las Liliopsida. Las formas de vida predominantes en cuanto a familia se reúnen en seis categorías: hierbas con 29 familias (34%, arbustos con 14 (16%, árboles con 9 (11%, trepadoras/lianas con 9 (11%, hierbas/arbustos con 7 (8% y hierbas/epífitas con 5 familias (6%. Se encontró que además de albergar una diversidad excepcional y brindar utilidad económica local, muchas de las especies no están registradas en las vertientes occidentales andinas del norte y del sur. Por la importancia que posee y por ser uno de los últimos bosques relictos existentes, la conservación legal de éste frágil ecosistema es urgente. Se propone que debe ser declarada zona de protección o intangible y que su conservación debe ser integral, involucrando a científicos, Estado, gobiernos regionales, gobiernos locales y población.

  20. Investigação dos efeitos citotóxico, genotóxico e antibacteriano dos taninos isolados de Anacardium Occidentale Linn e Anadenanthera Macrocarpa (Benth.) Brenan em linhagens de Streptococcus associados ao biofilme dental

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    Araújo, Josenildo Segundo Chaves de

    2013-01-01

    As plantas são uma rica fonte de novas moléculas biologicamente ativas com reconhecido potencial no desenvolvimento de medicamentos. O Anacardium occidentale Linn é popularmente conhecida como 'cajueiro', é uma planta da família das Anacardiaceae, originária do Nordeste do Brasil e popularmente conhecida por suas propriedades antiinflamatórias e cicatrizantes. O Anadenanthera macrocarpa (Benth.) Brenan ou o "angico" é pertencente a família das Mimosaceae, distribuída pelas r...

  1. Study of rheological behavior of cashew apple (Anacardium occidentale, l., acerola (Malpighia emarginata, d.c. and mango (Mangifera indica, l. pulpsEstudo do comportamento reológico de polpas de caju (Anacardium occidentale, l., acerola (Malpighia emarginata, d.c. e manga (Mangifera indica, l.

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    Evânia Altina Teixeira de Figueiredo

    2012-04-01

    Full Text Available The knowledge of the fruit tropical pulps rheological behavior have a great importance for the consumers and food process industries. In this work it was studied the rheological behavior of the cashew apple (Anacardium occidentale, L., mango (Mangifera indica, L and acerola (Malpighia emarginata, DC single strength, frozen and pasteurized pulps. The experimental measurements were carried out in concentric cylinder rheometer and the experimental data were fitted to models of Ostwald-de-Waelle and Bingham. There were evaluated chemical, physical-chemical and microbiological parameters. In this work, in order to adjust the rheological parameters, the model that presented the best description was the Ostwald-de-Waelle for the mango and acerola pulps, and Bingham for cashew apple pulp. The samples presented non-Newtonian and pseudoplastic character. The chemical and physicochemical analysis showed the nutritional value of the studied fruits. The microbiological results presented satisfactory hygienic and sanitary conditions in pulps processing.O conhecimento do comportamento reológico das polpas de frutas tropicais é muito importante para os consumidores e para a indústria de alimentos. Neste trabalho se estudou o comportamento reológico de polpas de caju (Anacardium occidentale, L., manga (Mangifera indica, L e acerola (Malpighia emarginata, D.C. integrais, pasteurizadas e congeladas. As medidas experimentais foram realizadas em reômetro rotacional de cilindros concêntricos e os dados experimentais foram ajustados aos modelos de Ostwald-de-Waelle e Bingham. Também foram avaliados os parâmetros químicos, físico-químicos e microbiológicos. No estudo, para o ajuste dos parâmetros reológicos, o modelo que apresentou maior ajuste foi o de Ostwald-de-Waelle para as polpas de acerola e manga e Bingham para a polpa de caju. As amostras apresentaram comportamento não-newtoniano e caráter pseudoplástico. As caracterizações químicas e f

  2. Avaliação da toxicidade subcrônica do extrato bruto seco de Anacardium occidentale Linn em cães - DOI: 10.4025/actascihealthsci.v28i1.1112 Evaluation of the subchronic toxicity of the crude dry extract of Anacardium occidentale Linn in dogs - DOI: 10.4025/actascihealthsci.v28i1.1112

    OpenAIRE

    Isabely de Souza Vera Cruz; Gracielle Carvalho Gomes; Maria Almerice Lopes da Silva; Miracy Muniz Albuquerque; Arquimedes Fernandes Monteiro de Melo; Vanessa Ribeiro Leite; Jane Sheila Higino

    2006-01-01

    A busca de novos medicamentos tem levado ao desenvolvimento de novos fármacos que sejam eficientes e destituídos de toxicidade. Uma das fronteiras nessas pesquisas são os medicamentos fitoterápicos. No Brasil, a Agência Nacional de Vigilância Sanitária (ANVISA) regulariza essas pesquisas e padroniza os procedimentos. A Resolução da Diretoria Colegiada (RDC) 48/2004, por exemplo, regulariza o registro de fitoterápicos. O Anacardium occidentale Linn está entre as plantas mais estudadas, devido ...

  3. Politiques de développement régional dans les Hautes Plaines occidentales algériennes : un bilan mitigé

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    Mohamed Hadeid

    2009-05-01

    Full Text Available L’Algérie a connu des mutations importantes grâce au programme de développement entrepris par l’Etat et ce, dès l’indépendance. Ce projet avait un double objectif : hisser le pays à un certain degré de développement économique et social et améliorer les conditions de vie des Algériens. Cependant, cette politique a beaucoup évolué au cours de l’histoire. En effet, l’intervention de l’Etat à travers sa politique d’industrialisation et de découpages administratifs n’a pas pu faire disparaître les disparités régionales héritées de la période coloniale. Les Hautes Plaines occidentales demeurent toujours en marge du développement. La politique d’aménagement du territoire du pays n’a fait qu’accentuer les déséquilibres déclenchés durant la période coloniale et ce, malgré les efforts consentis en matière d’équipement et de désenclavement. Le problème de dégradation de la steppe et la crise de l’activité pastorale en témoignent.Algeria has experienced important changes thanks to the development programme undertaken by the state from the independence. This project had dual purpose: Raising the country to economic and social level and improving living conditions of Algerian. However, this policy has undergone some slight changes throughout time as well as in level space. In fact, the interference of the state through industrialization policy and administrative apportionments were unable to bring to end regional disparities inherited from colonial period. Western high plains, for example, are still not benefiting from development. Regional and country planning policy has accentuated the imbalances which begun during colonial period and yet, the approved efforts as far as equipment and Opening up. The crisis presented by the degradation of the steppes and other pastoral activities is certainly the best evidence.

  4. ANACARDIUM OCCIDENTALE L.: PROSPECÇÃO TECNOLÓGICA APLICADA À TECNOLOGIA DE COMPOSTOS BIOATIVOS EM PRODUTOS ALIMENTÍCIOS

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    Lília Barretto

    2014-12-01

    Full Text Available O agronegócio mundial do caju movimenta cerca de 2,4 bilhões de dólares por ano. No Brasil, a região Nordeste responde por mais de 95% da produção, com divisas na ordem de 220 milhões de dólares anuais. Apesar da importância desta atividade agroindustrial, observa-se que o seu potencial econômico permanece pouco explorado, principalmente com relação ao aproveitamento do pedúnculo. Além de rico em nutrientes, o caju é uma importante fonte de compostos bioativos, os quais podem ser usados na elaboração de produtos funcionais. Este trabalho apresenta um mapeamento tecnológico sobre as potencialidades do caju referentes às tecnologias de compostos bioativos baseado na evolução das competências tecnológicas traduzidas através dos depósitos de patentes. Para a realização desta pesquisa, utilizou-se a base de dados Derwent Innovations Index. Mesmo sem apresentar geografia nem clima favoráveis para a produção de caju, o Japão liderou os rankings apresentados neste estudo, com 14 pedidos quando realizada a pesquisa pela palavra-chave Anacardium occidentale e 04, quando usado o termo cashew apple. A Universidade Federal do Maranhão é o destaque brasileiro, com dois depósitos relacionados à área farmacêutica. O único protocolo nacional referente a compostos bioativos extraídos a partir do caju foi depositado pela Universidade Federal de Minas Gerais em 2009 e ressalta um método laboratorial para análises de taninos extraídos de bebidas como o suco de caju. Os resultados demonstram uma área promissora para o desenvolvimento de patentes brasileiras relacionadas ao caju e seus compostos bioativos, hoje atualmente explorados por países não produtores desta fruta.

  5. DYNAMICS OF THE RUMINAL FERMENTATION IN SHEEP FEEDING WITH RATION CONTAINING DIFFERENT LEVELS OF CASHEW BY-PRODUCTS (Anacardium occidentale Efecto de la inclusion en los níveles crescentes del subproducto de caju (Anacardium occidentale L. en la dinámica de la fermentacion ruminal de ovinos DINÂMICA DA FERMENTAÇÃO RUMINAL EM OVINOS ALIMENTADOS COM RAÇÕES CONTENDO DIFERENTES NÍVEIS DE COPRODUTOS DE CAJU (Anacardium occidentale

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    Marcos Cláudio Rogério

    2009-07-01

    Full Text Available

    The study aimed to evaluate the inclusion of cashew by-product (Anacardium occidentale L. on the ammoniac nitrogen concentrations (N-NH3, pH and vollatile fatty acids concentrations (VFA of the ruminal fluid, in sheep that received diets containing the cited by-product. Twenty male, entire sheep had been distributed in four treatments with different levels of cashew by-product inclusion (zero; 19%; 38%; 52% in a randomized block design, in a split-plot project, having in the parcels the diets and the sub-parcels the times of collection (zero, two, five, eight hours after-feeding with five replications. PH was remained inside of the normal standards cited by same literature in the raised cashew by-product inclusions. Diets with zero and 52% had gotten the biggest concentrations of N-NH3. The molar ratio of the AGV in the ruminal liquid in the diets with cashew by-product was typical of rich diets in voluminous. The relation acetate: propionate was not affected by the inclusion of the cashew by-product. The inclusion of the cashew by-product in up to 19 % of the dietary total presented better resulted in that it says respect to the parameters analyzed in this work.

    KEY WORDS: Ammoniac nitrogen, Ovis aries, pH, parameters ruminate, ruminants, volatile fatty acids.

    El estudio apunto evaluar la inclusión del subproducto de caju (Anacardium occidentale en la concentración de nitrogeno amoniacal (N-NH3, el pH y las concentraciones de los acidos grasos vollatiles (AGV en el liquido ruminal de ovinos Chemo-type of essential oil of Ocimum basilicum L. from DR Congo and relative in vitro antioxidant potential to the polarity of crude extracts

    Institute of Scientific and Technical Information of China (English)

    Dorothe Dinangayi Tshilanda; Philippe Bila Babady; Damase Nguwo Vele Onyamboko; Damien Sha-Tshibey Tshibangu; Koto-te-Nyiwa Ngbolua; Philippe Vuka Tsalu; Pius Tshimankinda Mpiana

    2016-01-01

    Objective: To carry out a phyto-chemical characterization of essential oil from Ocimum basilicum L. (O. basilicum) harvested in DR Congo and to assess the antioxidant potential of crude extracts with respect to the polarity for comparison reason. Methods: The phyto-chemical characterization of essential oil produced by hydro-distillation was performed by coupled gas chromatography-mass spectrometer analysis and the antioxidant potential evaluation by in vitro 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity method. Results: A previously weighed amount of fresh leaves of O. basilicum produced 0.65% of essential oil that led to the identification of a set of 84.44% out of 99.98% as major com-pounds (> 1.5%). The chemo-type of this essential oil was linalool-methyl chavicol. Chemical components of oil were characterized by oxygenated aromatic hydrocarbons (46.00%) and oxygenated monoterpenes (26.75%). With respect to the amount of compo-nents, methyl chavicol also known as estragole (35.72%) constituted the very large quantity afterward linalool (21.25%) and then epi-a-cadinol (8.02%), a-bergamotene (6.56%), eugenol (4.60%), 1,8-cineole (4.04%), germacrene D (2.06%), thymol (1.64%), and (E)-citral (1.55%), respectively. Essential oil exhibited antioxidant potential and IC50=(1.180 ± 0.015) mg/mL. Non-polar crude extracts yields were low compared to the one of polar extracts. Only methanol and ethyl acetate had considerably manifested antioxidant potential with IC50 values equal to (0.025 ± 0.013) mg/mL and (0.085 ± 0.012) mg/mL, respectively. As concerns to IC50 values, essential oil was less active than methanol and ethyl acetate extracts. The methanol crude extract exhibited the highest activity. Non-polar extracts showed insignificant radical scavenging ability that did not allow assessing IC50 values. These results highlighted the occurrence of antioxidant potential compounds in polar media. Conclusions: Essential oil and crude extracts of O

  6. Bioactive compounds and total antioxidant capacity of cashew apples (Anacardium occidentale L. during the ripening of early dwarf cashew clones Compostos bioativos e atividade antioxidante total de pedúnculos de caju (Anacardium occidentale L. durante o amadurecimento de clones de cajueiro anão-precoce

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    Mônica Maria de Almeida Lopes

    2012-06-01

    Full Text Available Lately, tropical fruit consumption has increased due to a higher knowledge of its nutritional and therapeutic value. The aim of this work was to evaluate the antioxidant potential of cashew apples from different early dwarf clones during their ripening. The clones analyzed included: CCP 76, CCP 09, BRS 189 and BRS 265 in seven ripening stages. They were analyzed for vitamin C, total carotenoid, total anthocyanin, yellow flavonoids and polyphenol content and total antioxidant capacity. Clone BRS 265 ripe cashew apple presented the highest vitamin C content (279.37 mg x 100 g-1. The ripe BRS 189 cashew apple is colored bright red, and its total anthocyanin content was the highest (21.16 mg x 100 g-1. The yellow flavonoids content was higher for ripe CCP 76 and BRS 189 cashew apples with 56.32 and 50.75 mg x 100 g-1, respectively. The highest levels of extrable polyphenols and antioxidant capacity were observed in CCP 09 in the first five ripening stages. The antioxidant activity of cashew apples (Anacardium occidentale L. is mainly attributed to polyphenol content (r = 0.90; p Ultimamente, o consumo de frutas tropicais tem aumentado em razão de um maior conhecimento de seu valor nutricional e terapêutico. Neste trabalho, objetivou-se avaliar o potencial antioxidante de pedúnculos de cajus de diferentes clones de cajueiro anão precoce, durante o seu amadurecimento. Os clones analisados foram: CCP 76, CCP 09, BRS 189 e BRS 265, em sete estádios de amadurecimento. Foram analisados para a vitamina C total, antocianinas, carotenóides totais, flavonóides amarelos, teor de polifenóis e capacidade antioxidante total. O clone BRS 265 maduro obteve o maior teor de vitamina C (279,37 mg x 100 g-1. O pedúnculo maduro BRS 189, apresentou o maior conteúdo de antocianinas totais (21,16 mg x 100 g-1. O conteúdo de flavonóides amarelos foi maior para os clones CCP 76 e BRS 189 maduros, com 56,32 e 50,75 mg x 100 g-1, respectivamente. Os maiores teores

  7. Influence of gamma radiation on the levels of polyphenols and lethality of ethanol extracts of Anacardium occidentale Linn., against Biomphalaria glabrata; Influencia da radiacao gama na acao moluscicida de extratos de cajueiro em Biomphalaria glabrata

    Energy Technology Data Exchange (ETDEWEB)

    Santos, G.H.F.; Silva, E.B., E-mail: santosghf@hotmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Departamento de Energia Nuclear. Grupo de Radioprotecao e Radioecologia; Melo, A.M.M.A.; Lima, C.S.A [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Biofisica; Amorim, E.L.C.; Peixoto Sobrinho, T.J.S. [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Departamento de Farmacia

    2013-08-15

    Plant materials rich in phenolic compounds, such as Anacardium occidentale Linn., Have been used as alternatives to synthetic pesticides in Biomphalaria glabrata control programs, intermediate host of Schistosoma mansoni. Studies show that ionizing radiation can influence the content of phenolic compounds and thus their biological actions. The aim of this study was to evaluate the influence of gamma radiation of {sup 60}Co in polyphenol composition of hydroalcoholic extracts of bark and leaves of A. occidentale and evaluate the toxicity of these extracts to embryos and adults of B. glabrata. To achieve this goal this, the extracts were irradiated at 10 kGy, the controls being maintained from 0 kGy and positive (CaCO{sub 3}) and negative (H{sub 2}O). We quantified the total phenols by the Folin-Ciocalteau and tannins by precipitation of casein. Extracts were used at a concentration of 100 mg/L. The results showed that the radiation caused the changes to the leaves, the percentage of polyphenols and tannins, and the percentage of lethality in embryos and adults Biomphalaria glabrata, these percentages being: 13 ± 5 (0 kGy) and 27 ± 2.5 (10 kGy), and 36.67 ± 5.77 (0 kGy), and 56.67 ± 5.77 (10 kGy), respectively. Gamma radiation caused significant changes in the levels of polyphenols in the extracts of leaves of Anacardium ocidentale Linn., translated by the increased toxicity of this extract against embryos and adults of Biomphalaria glabrata. This indicates that gamma radiation can be used as an agent potentiating the toxicity of plant extracts on the alternate use of these materials as molluscicides. (author)

  8. Estimulantes químicos na extração da goma de cajueiro(Anacardium occidentale, L. Chemical stimulants in the extraction of the gum of cashew tree

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    Antônio Calixto Lima

    2001-06-01

    Full Text Available O trabalho foi instalado no campo experimental de Pacajus - Ceará - Brasil, pertencente ao Centro Nacional de Pesquisa de Caju (CNPAT/EMBRAPA, no decurso do ano agrícola 1991 / 1992, tendo como objetivo avaliar o uso de estimulantes químicos na extração da goma de cajueiro (Anacardium occidentale, L., bem como estudar sua interferência sobre safras futuras da referida matéria. Na pesquisa, foram testadas, através de esquema fatorial, concentrações de 0, 15, 30 e 45% de ácido sulfúrico, combinadas com um nível de dimetil sulfóxido (5% mais 5 níveis de ethephon que variaram de 0 a 20% do ácido 2 - cloroetilfosfônico. Obteve-se um aumento generalizado das produções de goma nos meses que se seguiram à primeira operação de estriamentos por efeito dos estimulantes aplicados. O ácido sulfúrico não interferiu na exsudação, enquanto a solução de 15% de ácido 2-cloroetilfosfônico promoveu a melhor resposta sobre a produção de goma.The work was carried out at the Experimental Field Station of the National Institute of Cashew Research (CNPAT/EMBRAPA in Pacajus County, State of Ceará, Brazil, in order to study the influence of chemical stimulants to extract gum from cashew tree (Anacardium occidentale, L., as well as to study its interference on future gum productions. Sulphuric acid concentrations of 0, 15, 30 and 45%, combined with concentrations of 0, 5, 10, 15 and 20% of 2-chloroethylphosphonic acid and 5% dimetil sulphoxide were tested in a factorial design. The influence of the stimulant over the future gum exudation was also assessed. A general increase of gum exudation was obtained in all months following the stimulant applications; sulphuric acid did not increase gum exudation in any of the used concentrations. On the other hand, a 15% concentration of 2-chloroethylphosphonic acid allowed the highest rate of gum exudation.

  9. Antimutagenic activity of cashew apple (Anacardium occidentale Sapindales, Anacardiaceae fresh juice and processed juice (cajuína against methyl methanesulfonate, 4-nitroquinoline N-oxide and benzo[a]pyrene

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    Ana Amelia Melo-Cavalcante

    2008-01-01

    Full Text Available Cashew apple juice (CAJ, produced from the native Brazilian cashew tree (Anacardium occidentale, and has been reported to have antibacterial, antifungal, antitumor, antioxidant and antimutagenic properties. Both the fresh unprocessed juice and the processed juice (cajuína in Portuguese has been shown to consist of a complex mixture containing high concentrations of anacardic and ascorbic acids plus several carotenoids, phenolic compounds and metals. We assessed both types of juice for their antimutagenic properties against the direct mutagens methyl methanesulfonate (MMS and 4-nitroquinoline-N-oxide (4-NQO and the indirect mutagen benzo[a]pyrene (BaP using pre-treatment, co-treatment and post-treatment assays with Salmonella typhimurium strains TA100, TA102, and TA97a. In pre-treatment experiments with strains TA100 and TA102 the fresh juice showed high antimutagenic activity against MMS but, conversely, co-treatment with both juices enhanced MMS mutagenicity and there was an indication of toxicity in the post-treatment regime. In pre-, co-, and post-treatments with TA97a as test strain, antimutagenic effects were also observed against 4-NQO and BaP. These results suggest that both fresh and processed CAJ can protect the cells against mutagenesis induced by direct and indirect mutagens.

  10. Caracterização anatômica e histoquímica de raízes e folhas de plântulas de Anacardium occidentale L. (Anacardiaceae

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    Ana Luísa Lopes Ernesto Reis

    2014-04-01

    Full Text Available Anacardium occidentale L. (Anacardiaceae é uma espécie típica do Cerrado do Brasil Central e possui valor socioeconômico. Objetivou-se caracterizar anatômica e histoquimicamente as raízes e folhas desta espécie em diferentes estádios iniciais de desenvolvimento. Para a caracterização anatômica, as amostras foram fixadas em FAA50, incluídas em parafina e submetidas aos procedimentos usuais para microscopia de campo claro. Foram realizados testes histoquímicos para detecção de lipídios totais, taninos, lignina, amido e terpenoides com grupo carbonila nas folhas, bem como teste para amido nas raízes em cortes frescos. A raiz apresenta epiderme unisseriada, floema com canais secretores e xilema tetrarco. Aos cinco dias após a germinação, a planta apresenta regiões com crescimento secundário e possui região medular na raiz, com função de armazenamento de grãos de amido. As folhas exibem epiderme unisseriada, com tricomas glandulares, cutícula espessa e estômatos paracíticos, em ambas as faces. O mesofilo é dorsiventral, e a nervura central apresenta feixes vasculares colaterais com canais secretores associados ao floema. A caracterização histoquímica da folha evidenciou lipídios totais e compostos fenólicos, entre eles taninos e lignina, em diferentes tecidos da folha. A plântula apresenta características que demonstram sua adaptação ao ambiente Cerrado, como cutícula espessa, mesofilo dorsiventral, crescimento secundário e presença de tricomas, mesmo sendo cultivada em condições de viveiro.

  11. Toxicity Of The Essential Oil Of The Cytral Chemotype Of Lippia Alba (Mill. N. E. Brown Toxicidad del aceite esencial de Lippia alba (Mill. N. E. Brown quimiotipo citral

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    Jesús Olivero-Verbel

    2010-06-01

    Full Text Available The essential oil (EO of Lippia alba (Mill. N. E. Brown (Verbenaceae has been traditionally used to treat several diseases. In this study, the acute toxic effects of the citral chemotype of L. alba EO were evaluated in mice. Animals were treated via intraperitoneal receiving the L. alba essential oil at doses between 50 and 2500 mg/kg, and the control group received sesame oil (vehicle. The EO induced dose-dependent neurotoxic effects at doses greater than 1000 mg/kg, including decreased locomotion, motor skills and muscle strength, hypotonia, dyspnea, kyphosis and convulsions. The EO was lethal at a dose of 2500 mg/kg. Animals receiving 1000 mg/kg were euthanized at the end of the treatment period and their blood and livers were collected for analysis. Mice exposed to L. alba EO presented signifcantly greater plasma alanine aminotransferase (ALT activities than the control group. Liver histological changes included mild infammation, in particular, an increase in nuclear size. Compared to vehicle control group, changes in expression for selected genes were signifcant for FABP5, a fatty acid transport related gene. In summary, the intraperitoneal administration of L. alba EO (citral chemotype causes neurological damage in mice at doses equal or greater than 1500 mg/kg, whereas at 1000 mg/kg, it generates mild liver damage. Therefore, the systemic use of this EO raises concerns about its safety.El aceite esencial (AE de Lippia alba (Mill. NE Brown (Verbenaceae ha sido utilizado tradicionalmente para tratar varias enfermedades. En este estudio, los efectos tóxicos agudos del AE de Lippia alba quimiotipo citral fueron evaluados en ratones. Los animales fueron tratados por vía intraperitoneal recibiendo el AE en dosis entre 50 y 2500 mg/kg de peso, y el grupo control aceite de sésamo (vehículo. Dosis superiores a 1000 mg/kg del AE mostraron efectos neurotóxicos incluyendo disminución de la locomoción e hipotonía, disnea, cifosis y

  12. Comparative seismic and petrographic crustal study between the Western and Eastern Sierras Pampeanas region (31°S Estudio sísmico y petrográfico cortical comparativo entre las Sierras Pampeanas Occidentales y Orientales (31°S

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    P. Alvarado

    2005-12-01

    Full Text Available The ancient Sierras Pampeanas in the central west part of Argentina are a seismically active region in the back-arc of the Andes. Their crystalline basement cored uplifts extend up to 800 km east of the oceanic trench over the flat subduction segment of the Nazca plate. Approximately 40 felt crustal earthquakes, are reported per year for this region. Historic and modern seismicity indicates that the Western Sierras Pampeanas (WSP have more crustal earthquakes of greater-size than the Eastern Sierras Pampeanas (ESP. Remarkable changes in composition and structure also characterize the WSP and ESP basements. We have quantitatively compared both regions using seismological constrains. A recent regional study of moderate earthquakes shows reverse and thrust focal mechanisms occurring at depths down to 25 km in the WSP. In contrast, the ESP have reverse and strike-slip focal mechanisms of shallower depths (Los antiguos bloques montañosos de las Sierras Pampeanas del centro-oeste argentino constituyen una región sísmicamente activa en la zona andina de trasarco. Estos bloques de basamento cristalino afloran hasta 800 km al este de la trinchera oceánica sobre el segmento de subducción horizontal. Más de 40 sismos «sentidos», son reportados por año para esta región. La distribución de la sismicidad moderna e histórica, muestra que las Sierras Pampeanas Occidentales experimentan más sismos de mayor magnitud que las Sierras Pampeanas Orientales. Geológicamente, existen marcados contrastes en la composición litológica y estructura del basamento en ambas regiones. Un estudio sismológico reciente indica que las Sierras Pampeanas occidentales son más activas sísmicamente que las orientales, con mecanismos focales inversos que alcanzan profundidades de hasta 25 km. Las Sierras Pampeanas orientales presentan mecanismos focales inversos y de desplazamiento de rumbo con profundidades focales < 10 km. Diferentes estructuras corticales de

  13. Variaciones texturales y movilidad geoquímica asociadas a milonitización: la zona de cizalla del granitoide El Tigre, Sierra de Pie de Palo, Sierras Pampeanas Occidentales, San Juan

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    Brígida Castro De Machuca

    2010-03-01

    Full Text Available Una faja de cizalla dúctil con foliación milonítica de dirección NEE y buzamiento al sudeste, afecta a un granitoide mesoproterozoico (Granitoide El Tigre: 31º31'30''S-68º15'12''O que forma parte del basamento cristalino de la Sierra de Pie de Palo, Sierras Pampeanas Occidentales. El análisis cinemático de la faja indica una componente principal de desplazamiento de rumbo con sentido de movimiento dextral. En el granitoide no deformado (protolito se preservan asociaciones minerales y texturas relícticas, ígneas y metamórficas, que son obliteradas por la deformación. La milonitización operó en un sistema abierto provocando la movilización (ganancia o pérdida de casi todos los elementos mayores y traza, incluyendo las tierras raras e isótopos de Rb/Sr y Sm/Nd. Los cambios químicos fueron controlados mayormente por transporte sintectónico de fluidos y por transformaciones mineralógicas producidas durante la milonitización (disminución del contenido de granate, biotita y minerales accesorios y neoformación de mica blanca en la matriz de las milonitas. Las variaciones isotópicas entre el protolito y las milonitas también serían resultado de la intervención de fluidos durante la deformación, con interacción variable entre fluido y rocas de caja. Los cambios texturales, mineralógicos y químicos experimentados por el granitoide durante la milonitización, permiten reconstruir su evolución tectono-metamórfica y las condiciones metamórficas imperantes. Las asociaciones minerales y microestructuras de deformación de las milonitas sugieren para la faja de cizalla del granitoide El Tigre condiciones propias de la facies de esquistos verdes, con temperaturas inferiores a los 400 °C. Este evento deformante tuvo lugar a los 473 ± 10 Ma durante la orogenia Famatiniana.

  14. Essai de synthèse stratigraphique et palynologique du système dévonien en Lybie occidentale Stratigraphic and Palynological Synthesis Attempt of the Denovian System in Western Libya

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    Massa D.

    2006-11-01

    Full Text Available De nombreux sondages profonds ont été réalisés lors des recherches pétrolières en Libye occidentale (bassin de Rhadamès. Simultanément, les zones d'affleurement ont été étudiées avec soin. Dans le présent travail, les auteurs présentent des conclusions stratigraphiques et palynologiques nouvelles. Les cycles sédimentaires qui constituent le Dévonien ont une épaisseur qui n'excède pas le millier de mètres. Ils sont subdivisés en sept formations qui couvrent l'intervalle stratigraphique allant du Praguien au Strunien. Ainsi, le Couvinien, le Givétien, le Frasnien et le Famennien ont été différenciés. Le Dévonien basal (Lochkovien est absent. Les corrélations latérales pour l'ensemble du bassin considéré sont satisfaisantes. Une trentaine de sondages ont été étudiés en palynologie. Ils ont montré une extraordinaire richesse en spores dans tout le Dévonien. II a été possible de différencier onze Palynozones, à partir des Spores et Chitinozoaires présents. En comparant les faunes et les microflores dévoniennes présentes en Libye, à celles connues en Europe, en Afrique du Nord et en d'autres régions, on obtient une biostratigraphie homogène et cohérente. Numerous deep boreholes have been drilled during oil prospection in western Libya (Rhadames Basin. At the same time, outcrop areas have been carefully examined. This article gives new stratigraphic and palynological conclusions. The sedimentary cycles making up the Devonian are no more than 1,000 meters thick. They are subdivided into seven formations, covering the stratigraphic interval from the Praguian ta the Strunian. In this way, the Couvinian, Givetian, Frasnian and Famennian are differentiated. The lowermost part of the Devonian (Lochkovian is missing. The lateral corrélations for the entire basin being considered are satisfactory. The palynology has been numerous in the Devonian. Eleven polynozones are differentiated on the basis of spores and

  15. Le Carbonifère de Lybie occidentale biostratigraphie et micropaléontologie position dans le domaine Téthysien d'Afrique du Nord The Carboniferous in Western Libya : Biostratigraphy and Micropaleontology

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    Massa D.

    2006-11-01

    Full Text Available Analyse des sondages et coupes de terrain effectués en Libye occidentale entre 1956 et 1966. Comparaison avec les formations d'lllizi en Algérie. Principaux résultats a Stratigraphiques : Treize biozones sont définies du Tournaisien supérieur au Moscovien inférieur. Tous les étages sont présents, en particulier le Bachkirien dont l'existence était mal établie. b Paléogéographiques : Identité des bassins de Rhadamès et d'lllizi, et sans doute de la plus grande partie du bassin de Djado-Mourzouk. Définition d'un golfe Libyque. Individualité de la baie de Serdelès, dont la limite nord peut être survie avec précision. c Paléoécologiques : Confinement biologique et pauvreté des microfaunes sauf en quelques niveaux. Importance des domaines supratidaux et intertidaux. Parti-cularités des formations stromatolithiques (à e Collenia » et oolithiques. Climat tropical sec, aride, dominant. d Systématiques : Données sur les Foraminifères, Algues, Aouigaliides et Calcifoliides. Deux créations, intéressantes du point de vue phylogénétique : Velebitella simplicissima VACHARD n. sp. (Algue Dasycladale , Conilolia africana VACHARD n. gen. n. sp. (Aouigaliide Ptychocladiidé. The Carboniferous from several boreholes and outcrops in western Libyan Basins is analyzed. Comparison is made with the Illizi Basin in Algeria. This study bas yielded new data on microfacies, microfossils, biostratig rophy and sedimentology, which con be used to attempt ta drow a rough outline of the paleogeographic development of these basins. The main results are: a Stratigrophy: From the uppermost Tournaisian into the Moscovien, thirteen biozones are defined. All stages are present, and the Bashkirian bas been identified with absolute certainty for the first time. b Paleogeography : Important paleogeographic facts become evident from the study of microfacies : - The Illizi and Rhadames Basins, and probably the major part of the Djado-Murzuk Basin, have the

  16. Deformación cuaternaria asociada al frente de levantamiento oriental de las sierras de Velasco y Ambato, Sierras Pampeanas occidentales Quaternary deformation associated with the eastern uplift front of the Sierras de Velasco and Ambato, western Sierras Pampeanas

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    Analía L. Casa

    2010-12-01

    Full Text Available Sobre el frente oriental de la sierra de Velasco y extremo sur de la sierra de Ambato, Sierras Pampeanas Occidentales, se han hallado nuevas evidencias de actividad tectónica cuaternaria en cercanías de la ciudad de La Rioja. Los rasgos reconocidos corresponden a distintos tramos reactivados de las fallas que delimitan los frentes serranos y a estructuras que afectan los depósitos cuaternarios sobre el piedemonte. la sierra de Velasco Oriental se encuentra marginada por fallas de rumbo NNE e inclinación al ONO, mientras que la sierra de Ambato austral presenta estructuras de rumbo NE que inclinan al NO. En el bloque Carrizal estas direcciones se interfieren originando un sector de mayor complejidad estructural. La deformación observada evidencia la persistencia de un régimen compresivo durante el Cuaternario al que se asocian fallas inversas de vergencia oriental a sudoriental. Estas fallas continúan elevando los cordones serranos y constituyen potenciales fuentes sismogénicas.Along the eastern front of the Sierra de Velasco and at the southern end of the Sierra de Ambato, western Sierras Pampeanas, have been found new evidences of Quaternary tectonic activity near La Rioja city. Observed evidences correspond to different reactivated sections of the faults that bound the mountain fronts, as well as to structures affecting the Quaternary deposits on the piedmont. The bounding faults of the Sierra de Velasco Oriental-front exhibit a NNE trend and WNW dip, while structures at the southernmost Ambato range are characterized by NE trend and NW dip. The neotectonic faults of the El Carrizal block are the result of the interference of both structural trends, resulting in a sector with higher structural complexity. The here described deformation show the persistence of a compressive regime during the Quaternary, with eastern to southeastern verging reverse faults. It is considering that these faults are driving the current uplift of the mountain

  17. Rapid Discrimination of Chemotypes of Cinnamomum camphora by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry%表面解吸常压化学电离质谱法快速判别樟树化学型

    Institute of Scientific and Technical Information of China (English)

    刘星星; 方小伟; 黄学勇; 张婷婷; 陈焕文; 罗丽萍

    2016-01-01

    采用表面解吸常压化学电离质谱(SDAPCI-MS)技术直接对5种化学型的樟树叶粉末片剂进行分析,获得其化学指纹谱图信息.采用主成分分析(PCA)、 聚类分析(CA)和反向传输人工神经网络(BP-ANN)对谱图信息进行分析,获得各化学型樟树叶粉末片剂的特征质谱信息,进而对不同化学型样品进行判别.结果表明,在正离子模式下,SDAPCI-MS能快速获取樟树的化学指纹谱图;PCA分析中的PC1,PC2和PC3贡献率分别为79.9%,12.9%和4.2%,共计97.0%.SDAPCI-MS结合CA和BP-ANN测试样本准确率均为100%,能够快速、有效地判别出樟树化学型.%Surface desorption atmospheric pressure chemical ionization mass spectrometry( SDAPCI-MS) was selected to detect five chemotypes of C. camphora leaves powder and the raw mass spectral fingerprints of the powder samples were obtained. Principal component analysis ( PCA ) , cluster analysis ( CA ) and the back propagation artificial neural network technology( BP-ANN) were used to analyze the spectral information. The results showed that the SDAPCI-MS technique could got mass spectral fingerprints of C. camphora quickly in positive ion mode. The contribution rates of PC1, PC2, PC3 were 79. 9%, 12. 9% and 4. 2%, respectively, with a total of 97. 0% in PCA. The accuracy of discrimination of CA and BP-ANN of SDAPCI-MS was 100%.

  18. Pharmacological properties of cashew (Anacardium occidentale)

    African Journals Online (AJOL)

    Rai Pablo Sousa de Aguiar

    2016-08-31

    Aug 31, 2016 ... to its economy, abundance and important chemical compounds. Net of cashew nut .... In the phytochemical analysis of cashew leaves, it is reported that it has ..... LCCI obtained by this process contains anacardic acid, cardanol, cardol .... phosphodiesterase isozymes and the design of selective inhibitors.

  19. Calchi di provenienza romanza nello sloveno occidentale

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    Mitja Skubic

    1997-12-01

    Full Text Available Il calco è il processo linguistico dove, contrariamente al prestito, si imita solo il contenuto semantico dell'espressione in una lingua straniera, o anche in un dialetto della stessa lingua, se si tratta di un calco semantico; oppure dove si imita la struttura in un altro sistema linguistico, e in tal caso abbiamo a che fare con un calco sintattico, strutturale. Bruno Migliorini ha dedicato al problema del calco un succinto studio, dove delimita chiaramente i due processi linguistici: »La forma più elementare di scambio linguistico che consegue a una simbiosi più o meno profonda tra due comunità linguistiche è quella del prestito, cioè I'imitazione più o meno esatta di vocaboli altrui, nella loro forma e nel significato.« Il calco, invece,è per il Migliorini l'imitazione dello spirito informatore, e questo procedimento »implica un più alto livello culturale e un maggior grado di bilinguismo.«

  1. Il nichilismo come destino della paideia occidentale

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    Michele Borrelli

    2013-06-01

    Full Text Available From Nietzsche to Heidegger, it seems that the will to power and the technique are not, if not the way today’s nihilism is articulated, ultimate fulfillment of the history of being as metaphysics but not as being. If these are the preconditions of the intrusiveness and of the inevitability nihilistic of Western metaphysics, which paideia today is still thinkable?

  2. A Study on the Chemical Components in Essential Oil from Leaves of Cinnamomum kanehirae and Chemotype Divisions%牛樟叶精油化学成分分析及类型划分研究

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    杨海宽; 章挺; 汪信东; 温世坊; 国颖; 江香梅

    2016-01-01

    从5年生实生牛樟植株上采集叶样,水蒸汽蒸馏法提取牛樟叶挥发性精油,采用 GC-MS 技术对叶精油中的化学成分进行定性、定量分析。按叶精油中第一主成分进行化学类型划分,牛樟可初步划分为4种类型:桉叶油素型、异橙花叔醇型、芳樟醇型和肉豆蔻醛型。不同化学类型叶精油化学成分组成及得率均存在较大差异,异橙花叔醇型牛樟叶精油中特有化学成分共13种,桉叶油素型牛樟叶精油中特有化学成分共2种,芳樟醇型牛樟叶精油中特有化学成分共2种,肉豆蔻醛型牛樟叶精油中特有化学成分为1种,4种化学类型精油中所共有的化学成分共12种。叶精油中第一主成分平均含量大小依次排序为:芳樟醇型(68.71%)、桉叶油素型(57.38%)、异橙花叔醇型(37.91%)、肉豆蔻醛型(33.75%);4种化学类型叶精油平均得率大小依次排序为:桉叶油素型(1.28%)、异橙花叔醇型(0.19%)、芳樟醇型(0.04%)、肉豆蔻醛型(0.01%)。牛樟中的桉叶油素型与樟树中的油樟类型相似,牛樟的异橙花叔醇型与樟树的异樟类型相似,牛樟的芳樟醇型与樟树的芳樟类型相似。不同的是,牛樟有一种肉豆蔻醛型化学类型,而樟树有龙脑樟和脑樟类型。但总体而言,牛樟与樟树相似化学类型叶中的精油含量,前者普遍低于后者。%Essential oil from leaves of 5-year old Cinnamomum kanehirae seedlings were extracted by steam distillation method.The chemical components and contents of the essential oil were analyzed by combined gas chromatography⁃mass spectrometry.Based on the first principal component of essential oil the chemotype of the tree was preliminary divided into 4 types:eucalyptol type,iso⁃nerolidol type,linalool type and tetradecanal type.The chemical components and yields of essential oil were significant

  3. Carboniferous Ostracodes in the Rhadamès Basin of Western Libya: Paleoecological Implications and Comparison with North America, Europe and the Ussr Les Ostracodes d'âge Carbonifère du bassin de Rhadamès de Libye occidentale : implications paléoécologiques et comparaison avec l'Amérique du Nord, l'Europe et l'URSS

    Directory of Open Access Journals (Sweden)

    Bless M. J. M.

    2006-11-01

    Full Text Available Carboniferous ostracode assemblages from two boreholes in the Rhadamès Basin of western Libya are compared with ostracode assemblages of the same age in North America, Western Europe and the USSR. The assemblages of the M'rar and Assedjefar Formations are characteristic of intertidal environments. These are compared with intertidal ostracode faunas from the late Devonian to early Dinantian of Belgium, and with near-shore faunas from the Upper Carboniferous of Spain and northwestern Europe. The assemblage of the Dembaba Formation is characteristic of a substital environment. Similar ostracode faunas have been described from subtidal or offshore deposits in the Devonian and Carboniferous of North America, Western Europe and the USSR. One genus (Rhadamesella and nine species (Mammoides bouckaerti, Monoceratina kockeli, Marginohealdia africana, Marginohealdia paprothae, Paracavellina fernetae, Cribroconcha streeli, Graphiadactyllis beckeri; Rhadamesella rhadamesensis and Rhadamesella bourdoni are new. Des associations d'Ostracodes d'âge Carbonifère sont étudiées et comparées avec des associations similaires d'Amérique du Nord, d'Europe occidentale et d'URSS. Le matériel étudié provient de deux sondages pétroliers implantés dans le bassin de Rhadamès en Libye occidentale. Les associations présentes dans les Formations M'rar et Assedjefar sont caractéristiques d'environnements intertidaux. Elles peuvent être comparées avec des faunes d'Ostracodes intertidaux connues du Dévonien supérieur au Dinantien inférieur de Belgique et avec des faunes côtières connues dans le Carbonifère supérieur d'Espagne et d'Europe nord-occidentale. L'association d'Ostracodes de la Formation Dembaba est caractéristique d'un environnement subtidal. De semblables faunes d'Ostracodes ont été décrites qui provenaient de dépôts subtidaux ou marins du Dévonien et du Carbonifère d'Amérique du Nord, d'Europe occidentale et d'URSS. Un genre

  4. Avaliação agronômica e identificação de quimiotipos de erva cidreira no Distrito Federal Agronomic evaluation and identification of Lippia alba chemotypes from Distrito Federal, Brazil

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    Hermes Jannuzzi

    2010-12-01

    production. A field assay was carried out in the rural area of Distrito Federal and the following parameters were analyzed: flowering period, growing habit, foliar area, length of the main branch, fresh and dry weight of the biomass (leaves and branches, essential oil content and profile of the constituent volatile oils. Essential oil was extracted using a modified Clevenger apparatus and the constituent volatile oils were analyzed by gas chromatography and GC/MS. The experimental design was randomized blocks with three plants per plot. The following chemotypes were reported: citral-limonene, citral-myrcene, limonene-carvone, citral, linalool, myrcene and linalool-limonene. Higher levels of linalool were found in genotype L.16 (89.8%; myrcene in L.37 (47.6%; limonene in L.27 (36.0%; carvone in L.27 (46.9% and citral in L.17 (56.7%. The genotypes with the highest leaf area and leaf length of the main branch seem to be correlated with the best yield of essential oil and the higher level of linalool. The yield of essential oil was inversely proportional to the dry weight of biomass. The accessions which presented the highest average yield of the major essential constituent oils were: L.16 (0.77 g pl-1 as a source of raw material for linalool production; L.17 (0.17 g pl-1 as source of myrcene; L. 27 as source of limonene (0.17 g pl-1 and carvone (0.21 g pl-1; and L.38 (0.24 g pl-1 as a citral source.

  5. Mare Occidentale Mare Occidentale: the adventure of imagining the Atlantic Ocean through sixteenth century maps

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    Carla Lois

    2007-11-01

    Full Text Available Desde el descubrimiento de América y a lo largo de todo el siglo XVI, una de las principales preocupaciones de los cartógrafos fue el dibujo cada vez más preciso de las costas del continente americano y de las islas atlánticas. Los estudios de los mapas de la época suelen concentrarse en los ensayos que los cartógrafos hicieron para diseñar las tierras nuevas, en especial, de América. En ese sentido, el océano Atlántico parecía ser el espacio que se definía por la negativa: aquello que no era tierra y que quedaba entre América y Europa. El Atlántico comenzó a ser objeto de diversas valoraciones simbólicas que fueron relevantes en la reorganización del imaginario geográfico de la época. El Atlántico, lejos de ser un espacio “hueco”, era parte de los territorios nuevos que se estaban conquistando y, así, fue integrado, con sus singularidades, a la nueva imagen del mundo. En este trabajo proponemos explorar los modos en que el Atlántico fue cartografiado en los mapamundis europeos del siglo XVI para analizar las formas en que este océano fue moldeado dentro del imaginario de un mundo geográfico que rápidamente se expandía y transformaba.Since the discovery of America and throughout the sixteenth century, one of the main concerns of cartographers was to draw with increasing precision the coasts of continental America and the Atlantic islands. The study of several maps from that time is included in essays developed by cartographers to outline the new land, especially in the case of America. In this sense, the Atlantic Ocean seemed to be the space defined by the negation of the above: that was not land and which filled up the space between America and Europe. The Atlantic Ocean started to be the object of a number of symbolic valuations which were relevant in the reorganization of the geographical imaginary space of the time. Far from being an “empty” space, the Atlantic Ocean, was part of the new territories being conquered and, as such, was incorporated to the new world’s image, along with its peculiarities. This work proposes to explore the ways in which the Atlantic Ocean was drawn in the European sixteenth century world maps, in order to analyze how this Ocean was molded within the imaginary geographic world which was undergoing a rapid transformation and expansion.

  6. Avaliação agronômica e química de dezessete acessos de erva-cidreira [Lippia alba (Mill. N.E.Brown] - quimiotipo citral, cultivados no Distrito Federal Agronomic and chemical evaluation of seventeen accessions of "erva-cidreira" [Lippia alba (Mill. N.E.Brown] - citral chemotype, cultivated at the Federal District, Brazil

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    H. Jannuzzi

    2011-01-01

    plant all over Brazil. Citral, a major constituent of L. alba essential oil, is responsible for its sedative activity. The aim of this study was to evaluate the agronomic behavior, content and yield of essential oil and citral in 17 accessions of L. alba from the germplasm bank of University of Brasília - UnB, Brazil. The experiment was carried out from 02/19/2005 to 03/18/2006, in red latosol, at the Federal District, Brazil. The adopted experimental design was in randomized blocks, with three replicates and three plants per plot. The following parameters were evaluated: leaf area, stem length, fresh mass (leaves and stems production, dry leaf mass production, essential oil and citral content, estimated yield of essential oil and citral per plant, besides characterization of the essential oil aromatic profile. Accessions L41 (0.75%, L45 (0.66% and L08 (0.62% showed the highest essential oil content. Accessions L45 (1.32 g pl-1 presented the highest essential oil yield, followed by L47 (0.73 g pl-1, L41 (0.67 g pl-1, L34 (0.67 g pl-1 and L33 (0.62 g pl-1. Citral content varied from 51.7% (L34 to 75.1% (L09, with an average of 63.6% and predominance of the isomer geranial (36.4% over neral (27.8%. Accession L45 (0.83 g pl-1 showed the highest citral yield. Thirteen accessions presented the chemotype citral; three, the chemotype citral-limonene, and only one had the chemotype citral-myrcene. The results evidenced the potential of accession L45 to produce citral, which can be used to improve the quality of "erva-cidreira" raw material in phytotherapy programs in the country or in breeding programs.

  7. Étude diachronique des changements du couvert végétal dans un écosystème montagneux par télédétection spatiale : cas des monts du Tessala (Algérie occidentale

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    Salah Eddine Bachir Bouiadjra

    2011-09-01

    Full Text Available Une meilleure compréhension de l'évolution des modes d'utilisation des sols et du couvert végétal est une préoccupation majeure pour les pays dont les écosystèmes subissent des dégradations sévères. En effet, les dynamiques d'occupation des sols ont des implications directes sur la disponibilité des ressources naturelles. Notre étude menée sur les monts du Tessala en Algérie occidentale, en utilisant l'indice de végétation par différence normalisée (NDVI, met en évidence l'ampleur des changements du couvert végétal entre 1987 à 2007. Une évolution régressive prononcée du couvert végétal est constatée au sud-est et au nord-ouest des monts du Tessala, tout particulièrement dans la "forêt de Tessala", sur les communes d'Aïn-Thrid, Tessala et Sehala. Les principaux facteurs contribuant à la régression du couvert végétal sont : la déforestation (plus de 26 délits de coupe par an, le surpâturage (420 délits de pâturage illicite par an, les incendies de forêts (plus de 20 incendies par an et l'érosion hydrique qui en résulte (environ 72 % des superficies sont concernées.A better understanding of the changing patterns of land use and land cover, is a major concern for countries where ecosystems are being severely degraded. Indeed, the dynamic feature of land use has important implications on natural resources. Our study conducted on the Tessala mountains, by using Normalized Difference Vegetation Index (NDVI, put in evidence the importance of changes in vegetation cover between 1987 and 2007. A pronounced regressive evolution is observed in south-east and north-west of the Tessala mountains, especially in the "forest of Tessala", in the communes of Aïn-Thrid, Tessala and Sehala. The main factors of environmental degradation, are : deforestation (over 26 offenses cut every year, overgrazing (420 crimes of illegal grazing every year, forest fires (more than 20 fires every year and resultant erosion (more

  8. Effect of organic fertilization on the shoot biomass of chemotypes of Lippia alba Efecto de la fertilización orgánica en la biomasa aérea de quimiotipos de Salvia morada (Lippia alba Mill. Efeito da adubação orgânica sobre componentes da produção em quimiotipos de erva-cidreira.

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    Gilberto Iris Souza

    2010-05-01

    Full Text Available

    El experimento se llevó a cabo en el área de cultivo orgánico de la Estación Experimental de lo Campus Universitario de Gurupi de la Universidad Federal de Tocantins – UFT, ubicado en la latitud 11° 43’ 45” S y Longitud 49° 04’ 07” W, con una altitud media de 280 m. Se evaluó el efecto de la fertilización orgánica en los aspectos vegetativos de tres quimiotipos de Salvia morada Lippia alba (Mill., N. E. Brown (quimiotipos I: mirceno-citral, II: citral-limoneno y III: carvona-limoneno. El experimento fue arreglado en un factorial (3 x 4 en el diseño de bloques al azar con tres repeticiones [(tres quimiotipos y cuatro dosis de abono orgánico (0, 2, 4 y 6 kg m-2 de estiércol bovino curado]. Las parcelas fueran composta por diez plantas. Las características evaluadas fueron: altura de la planta, número de hojas, el número de ramas principales, masa fresca y seca de hojas, la productividad de masa fresca y seca de hojas. El quimiotipo III se destacó en relación con otros. Las dosis de abono orgánico proporcionaran un aumento lineal de los promedios de las características evaluadas de las plantas. La dosis de 4 kg m-2 de estiércol bovino curado es la más adecuada para los tres quimiotipos.

    The experiment was conducted in and area of organic cultivation of the Sector of Olericulture of the Experimental Station of the University Campus of Gurupi, Universidade Federal do Tocantins – UFT (Federal University of Tocantins, located in the south latitude 11º 43’ 45” and west longitude 49º 04’ 07” with average height of 280 m. It was evaluated the effect of the organic fertilization over phytotechnical aspects in three chemotypes of Lippia alba (Mill. N. E. Brown (chemotypes I: myrcene-citral; II: citral-limonene and III: carvone-limonene. The experiment was factorial (3 x 4 in a randomized block design with three replications [(three

  9. Continuité ou discontinuité de sémentation marine mio-pliocène en Méditerranée occidentale. L'example du bassin de vera (Espagne méridionale Continuity Or Discontinuity of Io-Pliocene Marine Sedimentation in the Western Mediterranean. Example of the Vera Basin (Southern Spain

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    Montenat C.

    2006-11-01

    Full Text Available L'étude du bassin néogène de Vera apporte des données fort instructives pour l'interprétation du a phénomène Mio-Pliocène » en Méditerranée occidentale. a Au point de vue biostratigraphique La coupe de Cuevas del Almanzora permet d'observer, pour la première fois en Méditerranée, une succession continue de foraminifères planctoniques du Messinien au Pliocène inférieur. Les biozones à G. acostaensis, G. humerosa-G. duterérei, G. mediterranea-G. conomiozea et G. margaritae se succèdent sans hiatus évolutif. b Au point de vue géodynamique Le bassin de Vera montre une grande variété dans les modalités du passage Miocène-Pliocène, depuis des discordances à la périphérie du bassin jusqu'à une sédimentation vaseuse continue en son centre. L'étude des profils sismiques offshore montre de nombreux exemples comparables, en relation avec le comportement structural du substratum fragmenté en horst et graben mobiles. Des mouvements tectoniques distensifs ont eu lieu à la limite Miocène-Pliocène. Ils ont eu pour résultat un approfondissement relatif des bassins méditerranéens mais ceci ne représente qu'un épisode parmi d'autres d'un processus de distension inauguré au Miocène supérieur et qui s'est prolongé jusqu'au début du Quaternaire. II semble prudent en tous cas, de ne pas exagérer cet approfondissement de la mer au Pliocène. c Au point de vue paléogéographique L'exemple du bassin de Vera prouve la pérennité des conditions marines durant le Messinien du moins dans certaines parties de la Méditerranée. Ceci implique naturellement des liaisons continues avec l'Atlantique, et exclut un dessèchement général du bassin méditerranéen durant les temps messiniens. La paléogéographie de la zone bétique durant le Miocène supérieur est caractérisée par une extrême compartimentation du domaine marin constitué de bassins subsidents, communiquant par des seuils. Cette compartimentation s

  10. Resistencia antimicrobiana en Hospitales nor-occidentales de Nicaragua

    OpenAIRE

    Herrera, Karen; Espinoza, Meylin; Mejía, Yaoli; Zambrana, Luis Enrrique; Silva, Erasmo; Rojas, Jency; Gadea, Walter; Chavarria, Sergio; Hernandez, Mario; Ramirez, María Mercedes; Membreño, Juana María; Lara, María Eugenia; Saenz, J. E.; Valle, S.; Torrez, A.

    2007-01-01

    En los últimos años se ha observado un incremento de la incidencia de la Resistencia Antimicrobiana entre patógenos que causan infecciones intra-hospitalarias principalmente y también en la comunidad. La Resistencia antimicrobiana es un problema global de salud pública, promovido básicamente por el uso y abuso de los antibióticos. El fenómeno de la Resistencia antimicrobiana es un área prioritaria de investigación del Centro de investigación de enfermedades infecciosas y como parte de sus act...

  11. L’alun des oasis occidentales d’Égypte

    OpenAIRE

    2015-01-01

    Parmi les régions productrices d’alun, l’Egypte est citée plusieurs fois dans les textes de l’Antiquité, notamment au Ier siècle de notre ère par Pline (Naturalis Historia XXXV, 184) et par Dioscoride (De Materia Medica V, 106). On connaît aussi l’épisode plus ancien, rapporté par Hérodote (Histoires II, 180), des mille talents d’alun offerts par le pharaon Amasis pour la reconstruction du temple de Delphes détruit par un incendie en 548. La documentation écrite rassemblée par Guy Wagner pour...

  12. Valores occidentales en el discurso publicitario audiovisual argentino

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    Isidoro Arroyo Almaraz

    2012-04-01

    Full Text Available En el presente artículo se desarrolla un análisis del discurso publicitario audiovisual argentino. Se pretende identificar los valores sociales que comunica con mayor predominancia y su posible vinculación con los valores característicos de la sociedad occidental posmoderna. Con este propósito se analizó la frecuencia de aparición de valores sociales para el estudio de 28 anuncios de diferentes anunciantes . Como modelo de análisis se utilizó el modelo “Seven/Seven” (siete pecados capitales y siete virtudes cardinales ya que se considera que los valores tradicionales son herederos de las virtudes y los pecados, utilizados por la publicidad para resolver necesidades relacionadas con el consumo. La publicidad audiovisual argentina promueve y anima ideas relacionadas con las virtudes y pecados a través de los comportamientos de los personajes de los relatos audiovisuales. Los resultados evidencian una mayor frecuencia de valores sociales caracterizados como pecados que de valores sociales caracterizados como virtudes ya que los pecados se transforman a través de la publicidad en virtudes que dinamizan el deseo y que favorecen el consumo fortaleciendo el aprendizaje de las marcas. Finalmente, a partir de los resultados obtenidos se reflexiona acerca de los usos y alcances sociales que el discurso publicitario posee.

  13. "Tetsugaku Manga". Filosofia occidentale a fumetti in Giappone

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    La Marca, Paolo

    2010-01-01

    Full Text Available This paper aims to analyse some Japanese comics based on important works of Western philosophers. The focus is on the direct use of philosophical texts, by reference to the “Manga de Dokuha“ series published by East Press. Following a brief introduction of this series and its purposes, a more detailed analysis based on two well-known philosophical classics: Human, All Too Human, by Friederich Nietzsche, and The Prince, by Niccolò Machiavelli, is conducted.

  14. Anthelmintic efficacy of cashew (Anarcadium occidentale L.) on in ...

    African Journals Online (AJOL)

    Jane

    2011-08-24

    Aug 24, 2011 ... I. O. Ademola1,2* and J. N. Eloff1. 1Phytomedicine ... extract was then filtered through Whatman No 1 filter paper using a. Buchner funnel and ... extracted with an equal volume of butanol in a separating funnel to yield the water ... Albendazole was dissolved in dimethyl sulfoxide (0.3% DMSO) and diluted at.

  15. Numeros cromosomaticos de plantas occidentales 307-314

    OpenAIRE

    Bernal i Cid, Mercè; Montserrat Martí, J.M.

    1985-01-01

    Se estudiaron metáfases somáticas de meristemas radicales o de tejidos florales. La tinción se realizó con carmín acético para los tejidos florales y con el método de Feulgen para los meristemas radicales.

  16. Jóvenes de otros mundos: ¿Tribus urbanas? ¿Culturas juveniles? Aportaciones desde contextos no occidentales Jovens de outros mundos: Tribos urbanas? Culturas jovens? Contribuições dos contextos no occidentais Young people of other worlds: Urban tribes? Youth cultures? Contributions from nonwestern contexts

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    José Sánchez García

    2010-07-01

    Full Text Available A partir de una experiencia de campo en cuatro barrios de la ciudad de El Cairo, el autor traza una reflexión y hace un replanteamiento de las metodologías tradicionales instituidas para la investigación entre grupos generacionales en contextos occidentales. Aunque se defiende el carácter básicamente urbano de estas asociaciones, se discute la pertinencia de la aplicación de los conceptos de "tribu urbana" y "cultura juvenil" en escenarios culturales como los representados en sociedades que han sufrido una transición rápida a una modernidad impuesta por las redes transnacionales. En este sentido, el objetivo es la afinación de esas herramientas conceptuales ofreciendo una nueva perspectiva de las mismas que tenga en cuenta las peculiaridades históricas y sociales de la construcción de la categoría social "joven" en otros lugares. Por último, el texto intenta reivindicar a la antropología como una disciplina empírica y no apriorística.De uma experiência de campo em quatro distritos da cidade do Cairo, o autor levanta uma reflexão e um repensar das metodologias tradicionais instituídas para a investigação entre grupos geracionais em contextos ocidentais. Embora o caráter basicamente urbano destas associações seja defendido, discute à relevância da aplicação dos conceitos "da tribo urbana" e "de cultura jovem" em cenas culturais como representadas nas sociedades que se submeteram, em uma transição rápida, a uma modernidade imposta pelas redes transnacionais. Neste sentido, o objetivo é a afinação daquelas ferramentas conceptuais oferecendo uma perspectiva nova que considera as peculiaridades históricas e sociais da construção de "jovens" como categoria social em outros lugares. Finalmente, o texto tenta reivindicar a antropologia como uma disciplina empírica e não apriorística.From an experience of field in four districts of the city of Cairo, the author raises a reflection and a reframing of the instituted

  17. The p53-MDM2/MDMX axis - A chemotype perspective

    NARCIS (Netherlands)

    Khoury, Kareem; Popowicz, Grzegorz M.; Holak, Tad A.; Doemling, Alexander

    The protein-protein interaction (PPI) of the tumor suppressor p53 and its negative regulator MDM2 consists of the most intense studied PPI with a group of small molecular weight antagonists described and many more disclosed in patent literature. Due to the A-level structural insight into p53

  18. Diverse inhibitor chemotypes targeting Trypanosoma cruzi CYP51.

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    Shamila S Gunatilleke

    Full Text Available BACKGROUND: Chagas Disease, a WHO- and NIH-designated neglected tropical disease, is endemic in Latin America and an emerging infection in North America and Europe as a result of population moves. Although a major cause of morbidity and mortality due to heart failure, as well as inflicting a heavy economic burden in affected regions, Chagas Disease elicits scant notice from the pharmaceutical industry because of adverse economic incentives. The discovery and development of new routes to chemotherapy for Chagas Disease is a clear priority. METHODOLOGY/PRINCIPAL FINDINGS: The similarity between the membrane sterol requirements of pathogenic fungi and those of the parasitic protozoon Trypanosoma cruzi, the causative agent of Chagas human cardiopathy, has led to repurposing anti-fungal azole inhibitors of sterol 14α-demethylase (CYP51 for the treatment of Chagas Disease. To diversify the therapeutic pipeline of anti-Chagasic drug candidates we exploited an approach that included directly probing the T. cruzi CYP51 active site with a library of synthetic small molecules. Target-based high-throughput screening reduced the library of ∼104,000 small molecules to 185 hits with estimated nanomolar K(D values, while cross-validation against T. cruzi-infected skeletal myoblast cells yielded 57 active hits with EC(50 <10 µM. Two pools of hits partially overlapped. The top hit inhibited T. cruzi with EC(50 of 17 nM and was trypanocidal at 40 nM. CONCLUSIONS/SIGNIFICANCE: The hits are structurally diverse, demonstrating that CYP51 is a rather permissive enzyme target for small molecules. Cheminformatic analysis of the hits suggests that CYP51 pharmacology is similar to that of other cytochromes P450 therapeutic targets, including thromboxane synthase (CYP5, fatty acid ω-hydroxylases (CYP4, 17α-hydroxylase/17,20-lyase (CYP17 and aromatase (CYP19. Surprisingly, strong similarity is suggested to glutaminyl-peptide cyclotransferase, which is unrelated to CYP51 by sequence or structure. Lead compounds developed by pharmaceutical companies against these targets could also be explored for efficacy against T. cruzi.

  19. Deconstructing Delphinium: Violence and Flowers in Rousseau's "Emile" and Henke's "Chrysanthemum"

    Science.gov (United States)

    Kinard, Timothy

    2012-01-01

    This article is an attempt to contribute to the conversation about "go[ing] beyond all kinds of binary thinking" (Lenz Taguchi, "Going beyond the theory/practice divide in early childhood education: introducing an intra-active pedagogy," 2010, p. 50), especially the binary which positions "adults" and "children" as being powerful and powerless,…

  20. Estudo da atividade antimicrobiana dos ácidos anacárdicos do óleo da casca da castanha de caju (CNSL dos clones de cajueiro-anão-precoce CCP-76 e CCP-09 em cinco estágios de maturação sobre microrganismos da cavidade bucal Study of the antibacterial activity of anacardic acids from the cashew Anacardium occidentale nut shell oil of the clone of cashew-midget-precocious CCP-76 and and CCP-09 in five stages of maturation on oral microorganisms

    Directory of Open Access Journals (Sweden)

    Carlos Alberto de A. LIMA

    2000-12-01

    Full Text Available A atividade antimicrobiana dos ácidos anacárdicos do óleo da casca da castanha de caju (CNSL Anacardium occidentale (Anacardiaceae foi estudada sobre os microrganismos da cavidade bucal Streptococcus mutans ATCC 25175, Staphylococcus aureus ATCC 12598, Candida albicans ATCC 10231 e Candida utilis. Os ácidos anacárdicos obtidos dos extratos etílicos do CNSL apresentaram atividade antibacteriana contra os microganismos citados, porém a maior atividade inibitória ocorreu sobre a bactéria Gram positiva Streptococcus mutans, considerada predominante na cárie dentária. As cáries dentárias são uma das mais freqüentes doenças infecciosas nos países em desenvolvimento. Os elementos que influenciam na cárie dentária incluem o estado nutricional, a ingestão de açúcar e a presença da microbiota cariogênica.The antimicrobial activity of the anacardic acids of the cashew nut shell oil Anacardium occidentale (Anacardiaceae was studied on the oral microorganisms Streptococcus mutans ATCC 25175, Staphylococcus aureus ATCC 12598, Candida albicans ATCC 10231 and Candida utilis. The anacardic acids obtained from the ethyl extract of the cashew nut shell oil presented activity antibacterial against the mentioned microorganisms, but the most inhibitory activity occurred with the Gram positive bacteria Streptococcus mutans, which is known to be one of the main cause of tooth decay. The tooth decay is one of the most frequent infectious diseases in the countries in development. The elements that influence in the tooth decay include the nutritional state, the ingestion of sugar and the presence of the microflora cariogênica.

  1. Variaciones texturales y movilidad geoquímica asociadas a milonitización: la zona de cizalla del granitoide El Tigre, Sierra de Pie de Palo, Sierras Pampeanas Occidentales, San Juan Textural variations and chemical mobility during mylonitization: The El Tigre granitoid shear zone, Sierra de Pie de Palo, western sierras pampeanas, San Juan

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    Brígida Castro De Machuca

    2010-03-01

    Full Text Available Una faja de cizalla dúctil con foliación milonítica de dirección NEE y buzamiento al sudeste, afecta a un granitoide mesoproterozoico (Granitoide El Tigre: 31º31'30''S-68º15'12''O que forma parte del basamento cristalino de la Sierra de Pie de Palo, Sierras Pampeanas Occidentales. El análisis cinemático de la faja indica una componente principal de desplazamiento de rumbo con sentido de movimiento dextral. En el granitoide no deformado (protolito se preservan asociaciones minerales y texturas relícticas, ígneas y metamórficas, que son obliteradas por la deformación. La milonitización operó en un sistema abierto provocando la movilización (ganancia o pérdida de casi todos los elementos mayores y traza, incluyendo las tierras raras e isótopos de Rb/Sr y Sm/Nd. Los cambios químicos fueron controlados mayormente por transporte sintectónico de fluidos y por transformaciones mineralógicas producidas durante la milonitización (disminución del contenido de granate, biotita y minerales accesorios y neoformación de mica blanca en la matriz de las milonitas. Las variaciones isotópicas entre el protolito y las milonitas también serían resultado de la intervención de fluidos durante la deformación, con interacción variable entre fluido y rocas de caja. Los cambios texturales, mineralógicos y químicos experimentados por el granitoide durante la milonitización, permiten reconstruir su evolución tectono-metamórfica y las condiciones metamórficas imperantes. Las asociaciones minerales y microestructuras de deformación de las milonitas sugieren para la faja de cizalla del granitoide El Tigre condiciones propias de la facies de esquistos verdes, con temperaturas inferiores a los 400 °C. Este evento deformante tuvo lugar a los 473 ± 10 Ma durante la orogenia Famatiniana.A high-strain ductile shear zone trending NEE with southeasterly dipping mylonitic foliation, has been recognized affecting a Mesoproterozoic (ca. 1105 Ma

  2. Isolation and characterization of antioxidant and antimicrobial compounds from Anacardium occidentale L. (Anacardiaceae leaf extract

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    O.O. Ajileye

    2015-07-01

    This study concluded that the extracts and isolated compounds had strong antioxidant and moderate antibacterial activities and could be effective in the management of oxidative stress related diseases. These findings also justified the use of this plant’s extracts in folk medicine.

  3. Qualche considerazione sulla costante di correlazione tra il lungo computo Maya ed il calendario occidentale

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    Adriano Gaspani

    2013-06-01

    Full Text Available The remains of the Mayan civilization describe (among other things many observations of various astronomical phenomena observed by the Maya. The modern Celestial Mechanics allows us to calculate with great accuracy when astronomical phenomena occurred and were visible in the sky for a given location on Earth too far back in time. The Maya developed the very accurate calendar known as the Long Count, but when we want to synchronize it with the western one, the correlation between the two is surprisingly very uncertain. The two calendars can be connected each other by analyzing historical data and various astronomical phenomena of which we have a paper trail in the ancient texts that is sufficient to establish an appropriate chronology. The correlation between the Long Count and the Gregorian calendar has been studied by many authors who have obtained correlation values very different from each other (almost 50 different values, which differ from one another by hundreds of years, producing considerable uncertainty in Mayan history in relation to other civilizations. Astronomy can potentially solve the problem of the correct identification of the relationship, provided that the records are available from the observations performed and documented chronologically according to the Mayan Long Count. In the present work has been performed the statistical analysis of the 52 correlations at present known and used pointing out that at the present state of knowledge it is not possible to get the exact determination of the coefficient of synchronization (correlation between the Long Count and the Western calendar, but only an assessment of its probability distribution function, thus allowing you to assign a probability value to each value of the correlation that has been published. The statistical analysis of the 52 values at present known leads to an optimal value of the correlation constant of JDN 588159 with an uncertainty of about ±35 years.

  4. Differenze craniometriche in popolazioni di Volpe (Vulpes vulpes L. nel Mediterraneo occidentale

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    Agatino Maurizio Siracusa

    2003-10-01

    Full Text Available La Volpe rossa (Vulpes vulpes ha un ampio areale e una tassonomia complessa, ancora non ben definita; poco numerosi sono inoltre gli studi sulla craniometria delle popolazioni mediterranee. In questo studio abbiamo misurato 21 variabili di 78 crani appartenenti a popolazioni della Penisola italiana (22, della Sardegna (6, della Sicilia (8 e della Spagna (42. I dati sono stati sottoposti ad indagine statistica mediante tecniche univariate (t-test e analisi discriminante. I risultati del t-test tra Italia e Spagna mostrano differenze statisticamente significative per 13 variabili (11 per i maschi e 13 per le femmine, tra Italia e Sardegna per 17 variabili, tra Spagna e Sardegna per 17 variabili, tra Italia e Sicilia 7 variabili, tra Spagna e Sicilia 11 variabili e tra Sicilia e Sardegna 8 variabili. Dal confronto tra i sessi sono risultate differenti, in modo statisticamente significativo, 11 variabili per il campione della Penisola italiana e 15 per la Spagna; di queste variabili differenti statisticamente, solo otto risultano comuni. Per Sicilia e Sardegna non abbiamo effettuato nessun confronto, dato il basso numero di campioni. L?analisi discriminante applicata agli stessi dati craniometrici ha permesso di estrarre funzioni discriminanti capaci di distinguere in maniera statisticamente significativa (p<0,001 differenti clusters che non sempre però concordano sia con la sistematica classica, sia con le recenti indagini genetiche.

  5. Les transformations de l'image de Saladin dans les sources occidentales

    OpenAIRE

    Jean Richard

    2009-01-01

    Le personnage de Salāh al-din Yūsuf, officier kurde au service de Nūr al-dïn devenu maître de l'Égypte puis de Damas et enfin d'Alep, et fondateur de l'empire ayyoubide, a été fort controversé de son vivant avant de devenir pour la postérité « le plus pur des héros de l'Islam ». Pour ses adversaires, les Francs d'Orient, il a de bonne heure suscité leur curiosité, et ceux-ci ont recueilli des informations qu'ils ont utilisées, dès avant 1187, dans un Carmen de Saladino où l'aventurier qu'il é...

  6. Separation of cashew (Anacardium occidentale L.) nut shell liquid with supercritical carbon dioxide.

    Science.gov (United States)

    Smith, R L; Malaluan, R M; Setianto, W B; Inomata, H; Arai, K

    2003-05-01

    Cashew nut shell liquid (CNSL) represents the largest readily available bioresource of alkenyl phenolic compounds. In this work, separation of CNSL from the pericarp of the cashew nut with supercritical carbon dioxide was studied. In the initial extractions with CO(2) at 40-60 degrees C and at pressures from 14.7 to 29.4 MPa, low yields were obtained. However, when the extractions were performed with one or more intermediate depressurization steps, the yield of CNSL increased to as high as 94%. Most of the oil did not separate from the shell during the depressurization step, but was obtained during the subsequent repressurization. The CNSL extract had a clear light brownish pink color and exhibited no evidence of polymerization or degradation. The pressure profile extraction method proposed in this work increases the possible CNSL extraction yields and greatly reduces the amount of CO(2) required for CNSL separation.

  7. Characterization of alkyl phenols in cashew (Anacardium occidentale) products and assay of their antioxidant capacity.

    Science.gov (United States)

    Trevisan, M T S; Pfundstein, B; Haubner, R; Würtele, G; Spiegelhalder, B; Bartsch, H; Owen, R W

    2006-02-01

    In this study the content of anacardic acids, cardanols and cardols in cashew apple, nut (raw and roasted) and cashew nut shell liquid (CNSL) were analysed. The higher amounts (353.6 g/kg) of the major alkyl phenols, anacardic acids were detected in CNSL followed by cashew fibre 6.1 g/kg) while the lowest (0.65 g/kg) amounts were detected in roasted cashew nut. Cashew apple and fibre contained anacardic acids exclusively, whereas CNSL also contained an abundance of cardanols and cardols. Cashew nut (raw and roasted) also contained low amounts of hydroxy alkyl phenols. Cashew nut shell liquid was used for a basic fractionation of the alkyl phenol classes and the individual anacardic acids, major cardanols and cardols were purified to homogeneity from these fractions by semi-preparative HPLC and definitively identified by nano-ESI-MS-MS, GC-MS and NMR analyses. The hexane extracts (10 mg/ml) of all cashew products tested plus CNSL, displayed significant antioxidant capacity. Cashew nut shell liquid was the more efficient (inhibition=100%) followed by the hexane extract of cashew fibre (94%) and apple (53%). The antioxidant capacity correlated significantly (P4.0 mM). The data shows that of these substances, anacardic-1 was by far the more potent antioxidant (IC50=0.27 mM) compared to cardol-1 (IC50=1.71 mM) and cardanol-1 (IC50>4.0 mM). The antioxidant capacity of anacardic acid-1 is more related to inhibition of superoxide generation (IC50=0.04 mM) and xanthine oxidase (IC50=0.30 mM) than to scavenging of hydroxyl radicals. At present a substantial amount of cashew fibre is mostly used in formulations of animal or poultry feeds. The data presented in this study, indicates that this waste product along with CNSL, both of which contain high contents of anacardic acids, could be better utilized in functional food formulations and may represent a cheap source of cancer chemopreventive agents.

  8. Extraction of cashew (Anacardium occidentale) nut shell liquid using supercritical carbon dioxide.

    Science.gov (United States)

    Patel, Rajesh N; Bandyopadhyay, Santanu; Ganesh, Anuradda

    2006-04-01

    This work investigated the extraction of cashew nut shell liquid (CNSL) using supercritical carbon dioxide (SC-CO(2)). Effects of process parameters such as extraction pressure, temperature and flow rate of SC-CO(2) were investigated. The yield of CNSL increased with increase in pressure, temperature and mass flow rate of SC-CO(2). However, under different operating conditions, the composition of CNSL varied. The study of physical properties and chemical composition of the oil obtained through super critical fluid extraction (SCFE) showed better quality as compared to the CNSL obtained through thermal route. Experimental results were compared with diffusion based mass transfer model. Based on this simple model, extraction time was optimized.

  9. Pyrolysis and gasification of cashew nut (Anacardium occidentale L.) shell: liquid products characterization

    Energy Technology Data Exchange (ETDEWEB)

    Figueiredo, Renata Andrade; Figueiredo, Flavio Augusto Bueno; Sanchez, Caio Glauco; Sanchez, Elisabete Maria Saraiva [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Faculdade de Engenharia Mecanica. Combustion Lab.]. E-mails: flavioa@fem.unicamp.br; renataaf@fem.unicamp.br; caio@fem.unicamp.br; bete@fem.unicamp.br; Arauzo, Jesus; Sanchez, Jose Luis; Gonzalo, Alberto [University of Zaragoza (Spain). Aragon Institute of Engineering Research. Thermo-chemical Processes Group (GPT)]. E-mails: qtarauzo@unizar.es; jlsance@unizar.es; agonca@unizar.es

    2008-07-01

    The environment contamination with effluents generated in the biomass pyrolysis process has been waking up the scientific community's interest and concern in a larger number of countries, that are adopting measures to quantify and reduce the generated effluents. The pyrolysis and gasification are processes that can serve as alternative for the recovery of energy in the biomass usage. Considering that Brazil is one of the greatest world producers of biomass, the theme of the biomass usage in the generation of energy has been largely discussed. By the processes of pyrolysis and gasification, depending on the biomass type, the same can be transformed in fuel (liquid, char and gases in different proportions). However, the gases have a level of impurity that should be controlled to use it in a motor or turbine. The main impurities that should be controlled are tars, chars, ashes and nitrogenated compounds. The biomass used in this work is the cashew nut shell, from the Northeast of Brazil. In northeast there are industries that process the cashew nut which can use the cashew nut main reject (shell) as fuel, avoiding landfill sanitary deposit. By thermal conversion of the biomass in the pyrolysis and gasification process, it was quantified the production of solids (char), liquids (tar) and gases. It was evaluated the influences of the final temperature (800, 900 and 1000 deg C) and the use of N{sub 2} in pyrolysis case, and a mixture of N{sub 2} and vapor of water in the gasification case, in the amounts of char, tar and gas. The exhausted gas passes through a tar (liquid) condensation system, which consists of two glass condenser vessels cooled with a mixture of ice and water and an electrostatic precipitator. The liquid fractions are extracted with isopropanol and the sample is analyzed for CG-MS and CG-FID for the identification and quantification of the present compositions. Around 50 different composed have been detected in the liquid fraction obtained, most of them being present on very low concentrations. In order to facilitate the evolution study of the liquid composition fraction with the temperature, the compounds detected have been grouped according to their chemical nature. In this work, the classification of the compositions will be used proposed by Sanchez et al. (COBEM, 2007). It is observed that in the pyrolysis and gasification processes the liquid fraction production increases with the decrease of the temperature. The liquid fraction collected in the two processes presents predominance of oxygenated compounds. (author)

  10. Structures de l'apprentissage dans les pays de l'Europe Occidentale

    Science.gov (United States)

    Lengrand, Paul

    1982-06-01

    In West European countries, as in most modern societies, learning can be divided into three sectors. Informal education occupies the greatest space, both because of its duration and because it extends into every part of life. It happens in many and various ways and circumstances — from learning the language and socialisation in the family environment to the experiences of retirement and the third age. A large number of factors are involved, particularly married life, family responsibilities, work, the influence of mass media and participation in political activities. It is also the area of self-education. Formal education, dispensed by schools and universities, corrects and guides what is learned in the informal sector. It provides part of the necessary learning in the fields of the arts and of the sciences. However, it falls short so far as the evolution of ideas, morals and social behaviour are concerned. It also only imperfectly fulfils its function of democratization, and because of its concentration on matters intellectual, it does not promote the development of the diverse capacities of the whole person. Nonformal education resembles informal education in that it relates to life, and formal education in its structured character. It is the domain of educational innovation, especially in the context of social relationships. Through the harmonious combination of these three sectors of learning, the principles of a global and integrated lifelong education can be implemented.

  11. La fine della Bildung e della Paideia occidentale. Hommage an Jörg Ruhloff

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    Michele Borrelli

    2010-11-01

    Full Text Available The main topic of the article is to reformulate the concept of Bildung. Reformulation in this sense means analyzing the crisis of Social Sciences and, in consequence, their problematic approach to rationalism, which is strictly orientated to the logos of Natural Sciences, even in the field of normative and symbolic spheres. The analysis focuses upon two aspects: on the one side it discusses the external reasons of the crisis which have led to an increasing reversal of the idea of Bildung. On the other side the analysis takes into consideration the internal reasons (loss of educational/pedagogical theory for this reversal. This double centered investigation offers a revealing insight into the foundation of the educational logos which can not be described as rationalistic logos, but as logos of discourse. In consequence the educational logos requires a semantic reformulation of its main categories and means. The crisis of the foundation of theory of Educational Sciences indicates the general crisis of theory of Social ‘Sciences’, which are no longer aimed at Verstehen (comprehension but at data-based Empirical Sciences addicted to efficiency. The proposal made in this article is to return to reflection about educational thinking, or, in other words: to get away from ‘science’ and return to thinking.

  12. Análisis de subsidencia de la Cuenca de las Salinas, Sierras Pampeanas occidentales

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    Edgardo A. Azeglio

    2008-06-01

    Full Text Available En el presente trabajo se ha evaluado la subsidencia total de la cuenca de Las Salinas mediante diagramas tiempo - profundidad, considerando la subsidencia inicial, por carga y termotectónica. También se calculó la velocidad de subsidencia interválica en función del tiempo geológico. El área en estudio se ubica aproximadamente entre los paralelos 31°17´S y 32°40´S y los meridianos 66°38´W y 67°48´W en las cercanías de la localidad de Marayes, en el sitio denominado cuenca de Las Salinas. Los diagramas tiempo - profundidad permitieron relacionar cambios en la velocidad de subsidencia de la cuenca durante el proceso distensivo del Mesozoico y el actualmente compresional a la cual esta sometida, obteniéndose valores concordantes con áreas circundantes. Como resultado del análisis se encontró que la velocidad de subsidencia inicial fue de 0,0017 mm/año en el Carbonífero, la que fue aumentando lentamente hasta alcanzar los 0,00875 mm/año en el Cretácico. Este incremento disminuye sensiblemente durante el Cretácico superior, para posteriormente incrementarse abruptamente en el Terciario (0,043 mm/año. Este régimen se mantiene durante el Plioceno a Mioceno, alcanzando valores promedios de 0,269 mm/año, culminando con valores de 0,0973 mm/año. De la comparación entre ambos diagramas de subsidencia se observan distintos ritmos de sedimentación entre el sector norte y sur de la cuenca que podría ser la consecuencia de un fallamiento en el basamento. La comparación de estos valores con los obtenidos en la cuenca de Beazley indican que si bien los valores de subsidencia inicial y ritmo de sedimentación fueron similares. El inicio de la subsidencia en la cuenca de Beazley, se produjo con posterioridad (en el Triásico. La componente termotectónica en ambas cuencas es similar a pesar que el gradiente térmico normal de la zona aumenta sensiblemente en dirección sur.

  13. Crises hydrauliques et perceptions du risque environnemental en Méditerranée occidentale

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    Yves Luginbuhl

    2004-12-01

    Full Text Available Financé par le programme « Eau, environnement et sociétés » du département SHS du CNRS, ce programme a pour objectif, grâce à une meilleure articulation entre sciences sociales et sciences du milieu, d'apporter des connaissances nouvelles sur la perception du risque par les acteurs concernés, tel qu’il est déjà vécu dans certaines zones, ainsi que dans l'hypothèse d’une crise hydraulique plus générale. Il concerne 4 pays, dont 2 pays européens de la rive nord de la Méditerranée (Espagne, Fran...

  14. Il periodo sismico della Sicilia occidentale iniziato il 14 Gennaio 1968

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    L. MARCELLI

    1968-06-01

    Full Text Available The memoir begins with a brief report on the activity
    carried out by the Istituto Nazionale di Geofisica on the occasion of the
    earthquakes, which violently hit numerous villages in West Sicily as from
    January 14th 1968: the full development of the seismological crisis was unceasingly
    followed by the seismological Observatory in Rome, which documented
    the phenomenon with instrumental surveys and calculations. A
    few days after the first shocks, the I.N.G. provided for the installation of three
    seismological stations around the meizoseismal area (the station in Palermo
    which had been damaged by the first violent shocks was rearranged and two
    more were installed, one at Trapani and the other at Agrigento for the purpose
    of following the course of the long series of aftershocks even in their
    slightest manifestations.
    The memoir contains the results of observations carried out in respect
    of the more severe shocks (survey of registration times, magnitude and epieentral
    intensity and gives a graph indicating the recurring frequency of
    the earthquakes, day by day, up to June 10th 1968.
    Figure 5 shows the position of some epicentres calculated by the Istituto
    Nazionale di Geofisica (I.N.G., the Bureau Central International de Seismologie
    (B.C.I.S. and by the United States Coast and Geodetic Survey
    (U.S.C.G.S.. The epicentre map may be compared with the tectonic map
    of the area.
    The macroseismic feature of the phenomenon is duly emphasised.
    After a concise survey of the most important effects caused by the shocks
    of greatest intensity (a survey rendered most difficult by the frightening
    rapid succession of events the memoir sets forth the results obtained by
    means of studies and calculations based on the damages officially ascertained
    by the Genio Civile Offices.
    As it was impossible to draw a chart of isoseismical lines, a chart of
    lines of even damage (isoblabe has been drawn (Pig. 6 and it summarizes
    the global effects of the whole series of shocks with respect to buildings. It
    is followed by an accurate and detailed analysis of results achieved.
    Finally the seismic precedents of the localities affected are resumed,
    with particular reference to the Belice Valley and Palermo area.

  15. Registro fósil precuaternario de tetrápodos en los Pirineos occidentales

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    Ana Berreteaga

    2005-01-01

    Full Text Available El estudio de los niveles fosilíferos continentales y marinos del Cretácico Superior y del Terciario de la Región Vasco-Cantabrica (Sinclinorio Subcantábrico y cuencas surpirenaicas adyacentes (Cuenca de Pamplona, Sector Noroccidental de la Depresión del Ebro ha puesto de manifiesto su potencial paleontológico en restos fósiles de vertebrados. Algunos de estos yacimientos son de gran relevancia en el Cretácico final de Europa, incluyendo nuevas especies de tortugas, escamosos, cocodrilos, dinosaurios y mamíferos. En la misma cantera del yacimiento alavés de Zambrana, compuesta principalmente por reptiles y mamíferos, es de gran interés paleontológico y contribuye a un mejor conocimiento de las faunas del Eoceno superior de la Península Ibérica. En Zambrana se ha reconocido hasta la fecha varias nuevas especies de perisodáctilos ecuoideos. En el Neogeno, se han descubierto nuevas localidades del Mioceno (Rambliense, Aragoniense en las Bardenas Reales de Navarra y otras áreas colindantes de la Cuenca del Ebro, que han proporcionado restos de anfibios, escamosos, tortugas, cocodrilos, aves y varios grupos de mamíferos. Por otro lado, una notable variedad de icnofacies de aves y mamíferos ha sido hallada en diferentes afloramientos de los potentes depósitos marinos litorales (Eoceno superior y continentales (Oligoceno-Mioceno inferior de la Alta Navarra.

  16. Le identità deboli e la perdita del Padre nella società occidentale

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    Carlo Baratta

    2004-12-01

    Full Text Available Scopo di questo contributo è analizzare le problematiche della formazione di identità nella società contemporanea, problematiche trasversali, che riguardano la costruzione del sé, le reti di relazioni che si determinano in una società complessa e i contesti anche virtuali che in essa si costruiscono. Identità caratterizzate da alta volatilità e perciò deboli, identità che non danno senso alla vita, perché dipendono da stili di vita temporanei, difficilmente sequenziabili. L'articolo propone una rilettura di Pareto e di Gehlen, testi di Risè e Prandstraller hanno permesso di definire meglio la dimensione qualitativa delle identità deboli. L'affievolirsi della figura paterna, nella società contemporanea, sia essa postindustriale, postmoderna, neomoderna è una ragione evidente della formazione di identità deboli.

  17. Pirates et esclaves noirs dans les Indes occidentales espagnoles (XVIe - XVIIe siècles)

    OpenAIRE

    2015-01-01

    International audience; Le monopole accordé à l'Espagne par le Saint-Siège, détourné de sa finalité première, uniquement spirituelle, par la cupidité des conquérants et l'intérêt de la Couronne, se transforma rapidement en exclusivité d'exploitation du Nouveau-Monde. Le processus, bien connu, entraîna une forte chute démographique des peuples autochtones, d'abord dans les îles de la Caraïbe puis dans les deux vice-royautés du continent, justifiant ainsi le recours à la traite des Noirs. Avec ...

  18. The Muslim Emigration in Western Anatolia L’émigration musulmane de 1914 en Anatolie occidentale

    Directory of Open Access Journals (Sweden)

    Elçin Macar

    2012-05-01

    Full Text Available Avec les guerres balkaniques, toute la région apprit à connaître un nouveau concept : le nettoyage ethnique. Les États cherchant à homogénéiser leur population le firent de deux manières : par les traités ou par la force. Les statistiques démographiques devinrent ainsi un instrument politique et commença alors « la manipulation ethnique ». Parmi les Chrétiens anatoliens, les Grecs ottomans furent la première cible du CUP qui craignait de perdre « le dernier territoire restant ». Talat Pacha, ministre de l'Intérieur parla aux diplomates allemands de débarrasser le pays « des ennemis de l’intérieur ». Expulsions, terreur et déportations : entre 1912 et 1918, sur les deux rives de l’Égée, près de la moitié des 17,5 millions d’habitants ont été « déplacés ». Le legs le pire de cette période est la tendance des États à croire qu’ils ont le droit d’échanger ou de déporter leurs populations comme du bétail.With the Balkan Wars, the whole region was introduced to a new concept: Ethnic cleansing. States looking to homogenize their population did this in two ways: either by treaty or by force. Population statistics thus became one political instrument and then started the “ethnic engineering”. Among Anatolian Christians, Ottoman Greeks were the first target of the CUP who feared losing the “last remaining territory”. Talat Pasha, Minister of the Interior, talked to German diplomats about ridding the country of “internal enemies”. After expulsions, terrorism and deportations between 1913 and 1918, on both sides of the Aegean, almost half the population of 17.5 million changed places. The era’s worst legacy is the idea that states have a right to exchange or deport their populations like property.

  19. Les transformations de l'image de Saladin dans les sources occidentales

    Directory of Open Access Journals (Sweden)

    Jean Richard

    2009-05-01

    Full Text Available Le personnage de Salāh al-din Yūsuf, officier kurde au service de Nūr al-dïn devenu maître de l'Égypte puis de Damas et enfin d'Alep, et fondateur de l'empire ayyoubide, a été fort controversé de son vivant avant de devenir pour la postérité « le plus pur des héros de l'Islam ». Pour ses adversaires, les Francs d'Orient, il a de bonne heure suscité leur curiosité, et ceux-ci ont recueilli des informations qu'ils ont utilisées, dès avant 1187, dans un Carmen de Saladino où l'aventurier qu'il était à leurs yeux est présenté sous des traits peu recommandables. Son manque de fidélité envers ses maîtres est particulièrement souligné. Les coups très durs qu'il a portés aux Francs ont été assortis de traits de cruauté ; mais ce sont les manifestations d'un esprit chevaleresque, dont les valeurs étaient communes aux deux camps, qui ont vite retenu l'attention. Le trouvère Ambroise le regarde encore comme un ennemi fort capable de perfidie ; mais le traducteur de Guillaume de Tyr adoucit les remarques du livre qu'il mettait en français pour le rendre plus sympathique. Les textes de caractère épique où il apparaît dans la première moitié du XIIIe siècle (Estoires d'Outremer, Ordene de Chevalerie accentuent ce caractère. Mais c'est dans le second cycle de la croisade qu'on le pare des qualités les plus éminentes qu'on attend d'un chevalier. On lui prête des origines chrétiennes (La fille du comte de Ponthieu et on lui attribue le beau rôle, en insistant sur sa générosité et sur sa bravoure, en lui faisant arbitrer des controverses religieuses, en lui prêtant enfin une aspiration à une mort chrétienne, qui sont bien loin du portrait que faisaient de lui les Francs de la fin du XIIe siècle.

  20. Quelques Coléoptéres de l’Afrique occidentale française

    NARCIS (Netherlands)

    Fairmaire, L.

    1899-01-01

    Long. 5½ à 7 mill. — Ovatus, convexus, rufus, nitidus; capite brevi, antice utrinque fortiter foveolato; prothorace transverso, elytris haud angustiore, lateribus sat rotundato, punctulato, antice angustiore; scutello ogivali, fere ruguloso-punctato; elytris brevibus, ad humeros angulatis, sat gross

  1. Clastogenic and toxicological assessment of cashew (Anacardium occidentale) nut bark extracts in Wistar rats.

    Science.gov (United States)

    Owumi, Solomon E; Fatoki, John O; Gbadegesin, Michael A; Odunola, Oyeronke A

    2015-01-01

    Occupational exposures to environmental toxicants have been associated with the onset of skin lesions-including cancers. Identification and reduction of exposure to such compounds is an important public health goal. We examined the effect of cashew shell oil (CSO), used in skin tattooing for its potential to induce skin transformation in rats. Corn oil and CSO (25, 50, and 100%) were topically applied to depilated sections of Wistar' rat skin (groups: I-IV) for six weeks. Effect of treatments on serum transaminases activity, histological changes in hepatocytes and induction of micronuclei in the bone marrow were examined. In addition, CSO-induced hepatocyte proliferation was also quantified. All animals survived the course of the study. Reduced percentage change in body weight and physical trauma were observed in CSO-treated rat. The effects were more prominent in Group IV (100% CSO). Relative liver weights and number of hepatocytes (cells/mm(2)) increased significantly in groups II-IV relative to control (p 0.05) affected in treated groups. Hepatic histopathology revealed moderate sinusoidal congestion (group II), in addition to portal congestion in (group III), with mononuclear cellular infiltration (group IV) animals. In addition, CSO induced significant micronuclei formation of polychromatic erythrocyte (mPCEs) in the rat bone marrow (p tattoos in humans should be discouraged and further studies need to be conducted.

  2. La ferita inflitta sul corpo dalla Storia. Elsa Morante fra spiritualità occidentale e orientale

    Directory of Open Access Journals (Sweden)

    Lucia Dell'Aia

    2011-11-01

    Full Text Available Elsa Morante scholars have long been aware of the author’s profound knowledge of oriental philosophical and literary traditions but, until now, there has been no examination of the theoretical and formal impact of this on her work. It is also well known, as Garboli notes, that during the 1960s and 1970s, Morante was deeply affected by Simone Weil and her work Cahiers, with its summation of oriental spiritualism – especially Hinduism – and Western religious and philosophical traditions. The principle of Weil’s mysticism, of the soul penetrated by grace, of the divine light capable of defeating pesanteur (gravity, is indeed the result of her fusing the Christian doctrine of Divine Illumination – recalling St John of the Cross – with the mystic light of oriental thought. The aim of this paper is, mediating through Weil, to investigate which interpretation of mystical illumination, western or eastern, Morante embraced in the 1960s and ‘70s, and to analyze its impact on her work La Storia (History. According to Weil, the experience of succumbing to divine light is made possible through a meditation on the ordeal of the cross, the unconsoled affliction that strikes the soul and permits the process of ‘decreation’.  Morante’s interpretation of the Second World War and the wounds inflicted by it on humanity, that can only begin to heal through a spiritual process of decreation, can be read in the light of Weil’s philosophy.

  3. Tecnica e riproducibilità: gli esiti della metafisica occidentale e la nuova dimensione estetica

    Directory of Open Access Journals (Sweden)

    Antonio Martino

    2015-03-01

    Full Text Available AbstractNihilism coincides with the accomplishment of metaphysics which, in turn, culminates in the triumph of the scientific rationality. The essence of metaphysics, in its last accomplishment (nihilism, consists of the oblivion of being, while the essence of nihilism represents the awareness of that oblivious accomplishment. The shadow of the Being (Seiende covers the Being (Sein while the real facts lead the scientific-experimental thought determining, from Galileo on, the supremacy of the experimentally reproducible objectivity as well as of the scientific method on the actually productive subjectivity. The result is the so-called «technological planetary orientation»: lost the sense of the metaphysic question, philosophy comes to the end. In other words, after Nietzsche there is only science/technique. Therefore, how has all that weighed on the aesthetic dimension? The artistic productivity, though reflecting the torment of the human soul, towards the nothing of sensemiraculously tends to make the light of the Being (Sein appear from the shadow of the material Being (Seiende. Every originating artistic shine of the Being (Seinis an aesthetic expression from the pure creative human act which celebrates its own being as a dynamic bond of connections (inter-essewould say evocatively Kierkegaard-Climacus that cannot understand the origin but spread its form. Der Nihilismus entspricht der Erfüllung der Metaphysik, die wiederum im Triumph der wissenschaftlichen Rationalität. Das Wesen der Metaphysik, in seiner extremen Erfüllung (Nihilismus, besteht aus dem Vergessen des Seins und das Wesen des Nihilismus ist das Bewusstsein solcher vergessender Erfüllung. Der Schatten des Seiendes verdeckt das Sein und die Elemente der realen Welt (die Tatsachenlenken das wissenschaftliche, experimentelle Denken, das seit Galileo, die Hegemonieder experimentell reproduzierbaren Objektivität, und der wissenschaftlichen Methode über die effektive Subjektivität auslöst. Das Ergebnis ist die sogenannte „technologische/planetarische Ordnung“: geht der Sinn der metaphysischen Frage verloren, kommt die Philosophie zu einem Ende. Mit anderen Worten, nach Nietzsche gibt es nur Wissenschaft/Technik. Nun, wie hat all dies die ästhetische Dimension beeinflusst? Obwohl die künstlerische Produktivität das Leiden der menschlichen Seele angesichts des Nichts des Sinns wiederspiegelt, so neigt sie doch dazu, das Licht des Seins (wie durch ein Wunder aus dem Schatten des materiellen Seiende sichtbar werden zu lassen. Die Quelleeines jeden künstlerischen Schaffens des Wesens ist die ästhetische Manifestation des reinen kreativen Akt des Menschen, der  das eigeneSein als dynamischen Kern von Beziehungen zelebriert (inter-esse würde Kierkegaard-Climacus sagen der nicht im Stande ist, den Ursprung zu erfassen, sondern nur dessen Formen verbreiten.

  4. Astarté en Baria. Templo y producción entre los fenicios occidentales

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    López Castro, José Luis

    2005-12-01

    Full Text Available Defining the roots of Iberian sculpture has always been a controversial topic. In addition to the local circumstances and social needs, a crucial importance has been given to the Greek trade, that would have supplied local societies with specific techniques and styles. However, Phoenician influence is seen today as a major impulse, not only because stone sculptures were also employed on the colonial context of the Iberian Peninsula, but also because Phoenician colonization changed significantly the organization of local societies. Recent excavations have shown-precisely the importance of the Phoenician presence at the Iberian Mediterranean coast. Some sculptures representing bulls coming from Sagunto to the lower Segura river are studied here under this perspective. They are considered as the first evidences of Iberian sculpture at this area, and their dependence from oriental ideologies is proposed.Baria es una ciudad fenicia y romana del Sureste de España. En el presente trabajo se propone la identificación de un área excavada a finales del siglo XIX con un templo de Astarté. Para ello se analizan un conjunto de fuentes literarias de época romana relativas a la antigua Baria y su conquista por Escipión en la Segunda Guerra Romano-Cartaginesa, así como la iconografía de sus acuñaciones monetales. Se revisan hallazgos arqueológicos de las antiguas excavaciones así como algunos resultados obtenidos por el autor en investigaciones de campo modernas. Se analiza también el significado del culto a Astarté en Baria y el papel económico que desempeñó históricamente.

  5. Synthesis of a novel chemotype via sequential metal-catalyzed cycloisomerizations

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    Bo Leng

    2012-08-01

    Full Text Available Sequential cycloisomerizations of diynyl o-benzaldehyde substrates to access novel polycyclic cyclopropanes are reported. The reaction sequence involves initial Cu(I-mediated cycloisomerization/nucleophilic addition to an isochromene followed by diastereoselective Pt(II-catalyzed enyne cycloisomerization.

  6. The geographic distribution and complex evolutionary history of the NX-2 trichothecene chemotype from Fusarium graminearum

    Science.gov (United States)

    F. graminearum and 21 related species form a species complex (FSAMSC-1) characterized by production of type B trichothecenes. However, some F. graminearum strains were recently found to produce NX-2, a novel type A trichothecene, resulting from variation in the trichothecene biosynthetic enzyme Tri1...

  7. Antioxidative properties of Thymus vulgaris leaves: comparison of different extracts and essential oil chemotypes.

    Science.gov (United States)

    Chizzola, Remigius; Michitsch, Hanneliese; Franz, Chlodwig

    2008-08-27

    Thyme (Thymus vulgaris L., Lamiaceae) is a subshrub from the Lamiaceae family with plants that are rich in essential oils and antioxidative phenolic substances. Twelve accessions originating from southern France and the variety 'Deutscher Winter' were grown in an experimental field in eastern Austria. Leaf samples from these plants as well as from a commercial thyme rich in thymol were analyzed for their essential oil and the antioxidative potential in various extracts. The assays for antioxidative activity were the total phenolics according to the Folin-Ciocalteu method, DPPH decoloration, and Fe(3+) reduction (FRAP). Both extraction techniques used, in the water bath at 40 degrees C and in the ultrasonic bath at room temperature, proved to be efficient. The best results were obtained with 60% ethanol as extractant. In the comparison of the different accessions the less active and the most active of these extracts differed by factors of 2.1 and 2.6 in the total phenolics and FRAP assay, respectively, and by factors 1.5-2.0 in the DPPH assay. Rosmarinic acid accounted for 22-55% of the antioxidant activity in the ethanolic extracts. Essential oils with high proportions of the phenolic components thymol and/or carvacrol showed the highest antioxidant activity. Ethanolic extracts from the residues after distillation were considerably lower in antioxidant activity than the respective extracts from the dried leaves. Extracts with CH2Cl2 in the ultrasonic bath contained volatiles in proportions close to the essential oil but displayed very low antioxidant activity.

  8. Multicomponent assembly of diverse pyrazin-2(1H)-one chemotypes.

    Science.gov (United States)

    Azuaje, Jhonny; El Maatougui, Abdelaziz; Pérez-Rubio, José M; Coelho, Alberto; Fernández, Franco; Sotelo, Eddy

    2013-05-03

    An expedient and concise Ugi-based approach for the rapid assembly of pyrazin-2(1H)-one-based frameworks has been developed. This convergent approach encompasses skeletal, functional and stereochemical diversity, exhibiting an unusually high bond-forming efficiency as well as high structure and step economies. The method involves the use of readily available commercial reagents and is an example of the reconciliation of structural complexity with operational simplicity in a time- and cost-effective manner.

  9. COMPARATIVE, QUALITATIVE AND QUANTITATIVE CHEMOTYPIC CHARACTERIZATION AMONG NORTH INDIAN TRIBULUS TERRESTRIS

    Directory of Open Access Journals (Sweden)

    Kumar Ashwani

    2012-06-01

    Full Text Available In India, Several medicinal plant species are used in herbal drug industries, whereas Tribulus terrestris extract has an ancient tradition in folk medicine and in ayurveda as a diuretic, mood enhancer, antiseptic and anti-inflammatory agent. Due to few phytochemical and pharmacological studies, there are no quality criteria for this Tribulus terrestris species as raw material. In this work, we present unique fingerprints of six samples of Tribulus terrestris population relating to the presence of flavonoids, alkaloid and saponins. Qualitative analysis of the phytochemicals of methanolic extracts revealed the presence of carbohydrates, saponins, phytosterols, phenols, flavonoids and tannins in all the plants. Quantitative analysis showed that the crude saponin was the major phytochemical constituent present in highest percentage followed by crude tannin in all six plants. These chemical characterizations can provide, for example, authentication of samples, detection of adulterations, and differentiation between closely related species.

  10. Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi

    OpenAIRE

    Terezinha de Jesus Faria; Rafael Sottero Ferreira; Lidiane Yassumoto; José Roberto Pinto de Souza; Noemia Kazue Ishikawa; Aneli de Melo Barbosa

    2006-01-01

    An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive....

  11. Specific CLK inhibitors from a novel chemotype for regulation of alternative splicing

    DEFF Research Database (Denmark)

    Fedorov, Oleg; Huber, Kilian; Eisenreich, Andreas

    2011-01-01

    There is a growing recognition of the importance of protein kinases in the control of alternative splicing. To define the underlying regulatory mechanisms, highly selective inhibitors are needed. Here, we report the discovery and characterization of the dichloroindolyl enaminonitrile KH-CB19, a p...

  12. The Genetic Basis for 3-ADON and 15-ADON Trichothecene Chemotypes in Fusarium

    NARCIS (Netherlands)

    Alexander, N.J.; McCormick, S.P.; Waalwijk, C.; Lee, van der T.A.J.; Proctor, R.H.

    2011-01-01

    Certain Fusarium species cause head blight of wheat and other small grains worldwide and produce trichothecene mycotoxins. These mycotoxins can induce toxicoses in animals and humans and can contribute to the ability of some fusaria to cause plant disease. Production of the trichothecene 3-acetyldeo

  13. Affinities of Phylacia and the daldinoid Xylariaceae, inferred from chemotypes of cultures and ribosomal DNA sequences

    DEFF Research Database (Denmark)

    Bitzer, Jens; Laessøe, Thomas; Fournier, Jacques;

    2008-01-01

    . A comparison of their 5.8S/ITS nuc-rDNA sequences agreed in some important aspects with the above results: H. nicaraguense and H. polyporus appeared basal to a clade comprising Daldinia, Entonaema, and Ph. sagrana. The latter species appeared allied to D. caldariorum, but was distantly related to Pyrenomyxa...

  14. Incisterols, highly degraded marine sterols, are a new chemotype of PXR agonists.

    Science.gov (United States)

    Chianese, Giuseppina; Sepe, Valentina; Limongelli, Vittorio; Renga, Barbara; D'Amore, Claudio; Zampella, Angela; Taglialatela-Scafati, Orazio; Fiorucci, Stefano

    2014-05-01

    During the chromatographic purification of organic extracts obtained from Plakortis cfr. lita we obtained three highly degraded steroid derivatives, the known incisterol A2 (1) and the new incisterols A5 (2) and A6 (3). The new compounds were characterized basing on NMR and MS evidences along with comparison with model compounds. Incisterol A5 proved to bear a 17S-ethyl-15E,18-diene (incisterol numbering system) side chain, found for the first time in a marine organism. The new incisterols A5 and A6 proved to be potent inducers of transactivation of the pregnane X receptor (PXR) and they also stimulate the expression of CYP7A4 and MDR1 with a potency comparable to that of Rifaximin. These observations prompt to consider incisterols A5 and A6 as new potent agonists of PXR. On the other hand, the 17R-methyl analogue incisterol A2 shows only a poor PXR agonist activity. Molecular docking simulations elucidated the binding mechanism of the active incisterols in the ligand binding domain of PXR.

  15. Promising Aedes aegypti repellent chemotypes identified through integrated QSAR, virtual screening, synthesis, and bioassay.

    Science.gov (United States)

    Oliferenko, Polina V; Oliferenko, Alexander A; Poda, Gennadiy I; Osolodkin, Dmitry I; Pillai, Girinath G; Bernier, Ulrich R; Tsikolia, Maia; Agramonte, Natasha M; Clark, Gary G; Linthicum, Kenneth J; Katritzky, Alan R

    2013-01-01

    Molecular field topology analysis, scaffold hopping, and molecular docking were used as complementary computational tools for the design of repellents for Aedes aegypti, the insect vector for yellow fever, chikungunya, and dengue fever. A large number of analogues were evaluated by virtual screening with Glide molecular docking software. This produced several dozen hits that were either synthesized or procured from commercial sources. Analysis of these compounds by a repellent bioassay resulted in a few highly active chemicals (in terms of minimum effective dosage) as viable candidates for further hit-to-lead and lead optimization effort.

  16. Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype against phytopathogenic fungi

    Directory of Open Access Journals (Sweden)

    Terezinha de Jesus Faria

    2006-11-01

    Full Text Available An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied. The antifungal activity of eugenol was evaluated against a species of Alternaria isolated from tomato (A1 and Penicillium chrysogenum. The minimal inhibitory concentrations of eugenol were 0.16 and 0.31 mg/disc for Alternaria sp. (A1 and P. chrysogenum, respectively.O óleo essencial resultante da destilação por arraste a vapor das partes aéreas de Ocimum gratissimum e o extrato etanólico obtido do resíduo da destilação foram avaliados quanto à atividade antifúngica, utilizando-se o método de difusão em agar. O óleo essencial inibiu o crescimento de todos os fungos testados, incluindo os fitopatogênicos Botryosphaeria rhodina e duas espécies de Alternaria sp, enquanto que o extrato do resíduo da destilação não apresentou atividade. O óleo essencial foi, então, submetido ao método de bioautografia em TLC para detecção do composto ativo. O componente ativo foi isolado e identificado através da análise por cromatografia gasosa acoplada à espectrometria de massas como o eugenol, constituinte majoritário do óleo estudado. Ensaios de atividade antifúngica revelaram a atividade do eugenol contra Alternaria isolada de tomate (A1 e Penicillium chrysogenum. As concentrações inibitórias mínimas foram 0,16 mg/disco e 0,31 mg/disco para Arternaria sp (A1 e P. chrysogenum, respectivamente.

  17. Proteome-wide reactivity profiling identifies diverse carbamate chemotypes tuned for serine hydrolase inhibition.

    Science.gov (United States)

    Chang, Jae Won; Cognetta, Armand B; Niphakis, Micah J; Cravatt, Benjamin F

    2013-07-19

    Serine hydrolases are one of the largest and most diverse enzyme classes in Nature. Inhibitors of serine hydrolases are used to treat many diseases, including obesity, diabetes, cognitive dementia, and bacterial and viral infections. Nonetheless, the majority of the 200+ serine hydrolases in mammals still lack selective inhibitors for their functional characterization. We and others have shown that activated carbamates, through covalent reaction with the conserved serine nucleophile of serine hydrolases, can serve as useful inhibitors for members of this enzyme family. The extent to which carbamates, however, cross-react with other protein classes remains mostly unexplored. Here, we address this problem by investigating the proteome-wide reactivity of a diverse set of activated carbamates in vitro and in vivo, using a combination of competitive and click chemistry (CC)-activity-based protein profiling (ABPP). We identify multiple classes of carbamates, including O-aryl, O-hexafluoroisopropyl (HFIP), and O-N-hydroxysuccinimidyl (NHS) carbamates that react selectively with serine hydrolases across entire mouse tissue proteomes in vivo. We exploit the proteome-wide specificity of HFIP carbamates to create in situ imaging probes for the endocannabinoid hydrolases monoacylglycerol lipase (MAGL) and α-β hydrolase-6 (ABHD6). These findings, taken together, designate the carbamate as a privileged reactive group for serine hydrolases that can accommodate diverse structural modifications to produce inhibitors that display exceptional potency and selectivity across the mammalian proteome.

  18. Cinética e caracterização físico-química do fermentado do pseudofruto do caju (Anacardium occidentale L. Kinetic and physico-chemical characterization of cashew (Anacardium occidentale L. wine

    Directory of Open Access Journals (Sweden)

    Alberto B. Torres Neto

    2006-06-01

    Full Text Available The production of cashew apple wine has the purpose of minimizing the wastage in the Brazilian cashew production. Knowing that the cashew apple fermentation produces a good cashew wine, a study of alcoholic fermentation kinetics of the cashew apple and the physico-chemical characterization of the product were made. The cashew wine was produced in an stirred batch reactor. The results of the physico-chemical analysis of volatiles, residual sugars, total acidity and pH of cashew wine showed that their concentrations were within the standard limits established by the Brazilian legislation for fruit wines.

  19. Bioethanol production by cashew apple bagasse (Anacardium occidentale L.): comparison of acid diluted and alkali pre-treatments; Producao de bioetanol a partir da fibra do caju (Anacardium occidentale L.): comparacao entre o pre-tratamento acido e alcalino

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Tigressa H.S.; Pinheiro, Alvaro D.T.; Goncalves, Luciana R.B. [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil); Rocha, Maria V.P.; Macedo, Gorete R. de [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil)

    2008-07-01

    Due to the growing environmental awareness on the negative impact resulting from utilization of fossil fuels, and in the search for renewable energy sources, biofuels' interest as Bioethanol has rapidly expanded recently. In this context, lignocellulosic compounds have become center of attention as an abundant and economic alternative source of carbohydrates for ethanol production. In this study, cashew's bagasse acid hydrolysis was initially studied for glucose synthesis and its fermentation towards ethanol production. Sulfuric acid concentration, solids concentration and time were some of the factors evaluated. The highest glucose productivity value (162,9 mg.g de bagaco{sup -1}) , was obtained for 0,6 mol.L{sup -1} of sulfuric acid in an autoclave at 121 deg C for 15 min. For the fermentation of the hydrolyzed material by S. cerevisiae containing 16 {+-} 2,0 g.L{sup -1} of glucose metabolic, the yield and productivity obtained were 0,63 g-g glucose{sup -1} and 1,43 g.L{sup -1}h{sup -1} respectively. Ethanol concentration after 6 h of fermentation of this hydrolyzed was 11 g.L{sup -1}. In the best conditions of acid hydrolysis, a second pre-treatment with diluted sulfuric acid was performed to evaluate availability of hemicelluloses at 160 deg C and 180 deg C. For comparative purposes, alkali pre-treatment was evaluated under the conditions of 0,6 mol.L{sup -1}, 30% p/v of bagasse thermically treated at 121 deg C for 15 minutes. However, concentration of sugars was not sufficient for fermentation. Also, nitrogen supplementation of the hydrolyzed fraction did not influence significantly ethanol production. The results demonstrate that the hydrolyzed fraction from the acid pre-treatment of cashew's bagasse could be utilized for ethanol production. (author)

  20. CASHEW PULP MEALS (Anacardium occidentale L. FOR GROWING PIG: NUTRIENT METABOLISM AND PERFORMANCE PSEUDOFRUTO DO CAJUEIRO (Anacardium occidentale L. PARA SUÍNOS EM CRESCIMENTO: METABOLISMO DE NUTRIENTES E DESEMPENHO

    Directory of Open Access Journals (Sweden)

    Leonardo Atta Farias

    2008-04-01

    Full Text Available These researches was planned to evaluate the digestibility and metabolizability of nutrient and nitrogen balance of the cashew pulp for growing pigs, as well as to evaluate the performance of these animals fed with different levels of inclusion of this by-product in the diets. The physiological aspects of the animal and economic viability of the diets was evaluated. In the metabolism assay, four pigs were fed with a standard ration and others four received a test ration, with 30% of substitution of the standard ration for the cashew pulp. In the performance assay, forty animals were distributed in a randomized block design, with five treatments and four replications. The treatments consisted of the experimental rations with the levels 0%, 5%, 10%, 15% and 20% of inclusion of the cashew pulp. The values obtained for the digestibility and metabolizability coefficients of the protein and energy of the cashew pulp meals were 12.30% and 11.38%; 23.43% and 21.91%, respectively. The digestible and metabolizability energy of cashew pulp meals is 1.123 and 1.051 kcal/kg. The cashew pulp meals can be included in diets until the level of 20% of the ration, and this inclusion of the by-product increases the financial yield of the production.KEY WORDS: Alternative feed, nitrogen balance, temperature, weight gain. A pesquisa destinou-se a avaliar a digestibilidade, o metabolismo de nutrientes e o balanço de nitrogênio do pseudofruto do cajueiro para suínos em crescimento, bem como o desempenho desses animais alimentados com diferentes níveis de inclusão desse subproduto nas dietas. Avaliaram-se ainda os aspectos fisiológicos dos animais e a viabilidade econômica das dietas testadas. No ensaio de metabolismo, quatro leitões foram alimentados com uma ração referência e outros quatro com uma dieta-teste, que apresentou 30% de substituição da ração referência pelo farelo do pseudofruto do cajueiro. No ensaio de desempenho utilizaram-se quarenta animais, distribuídos em blocos ao acaso, com cinco tratamentos, quatro repetições. Os tratamentos consistiram de rações com os níveis de 0%, 5%, 10%, 15% e 20% de inclusão do pseudofruto do cajueiro. Os valores dos coeficientes de digestibilidade e dos metabolismos da proteína e da energia do pseudofruto do cajueiro obtidos foram 12,30 e 11,38%; 23,43 e 21,91%, respectivamente, sendo encontrados valores de 1.123 kcal/kg e 1.051 kcal/kg para energias digestível e metabolizável. O pseudofruto do cajueiro pode ser incluído nas dietas de suínos em crescimento até o nível de 20% da ração, e sua inclusão na forma de farelo melhora o rendimento financeiro da produção.PALAVRAS-CHAVES: Alimento alternativo, balanço de nitrogênio, ganho de peso, temperatura.

  1. Etica, filosofia e mediazione linguistica: dall’Etica della filosofia occidentale al codice deontologico della mediazione linguistica

    Directory of Open Access Journals (Sweden)

    Mette Rudvin

    2016-01-01

    Full Text Available Abstract – Over the last few years, the issue of professional ethics has received much attention in the field of interpreting and translation, and in particular in the field of Community Interpreting (CI or Public Service Interpreting (PSI. (In this chapter we will refer to CI or PSI in Italian as ‘Mediazione Linguistica’. Today, ‘ethics’ figures prominently in the literature, in international conferences, in interpreting courses, in translation/interpreting mailing lists as well as in the working lives of professional interpreters. As an object of theoretical inquiry (meta-ethics as well as a guide for human conduct (normative ethics, ethics has been a prime focus of Western moral philosophy since the time of the Ancient Greeks. This chapter situates the main ethical tenets of the CI/PSI interpreting profession within the framework of the main principles of moral philosophy, namely notions of ‘good’, ‘virtue’, ‘duty’, ‘responsibility’, ‘utility’ and ‘consequence of actions’. The three principal ethical tenets of CI/PSI discussed in this chapter, Accuracy, Impartiality and Confidentiality, were identified on the basis of a general literature review and more specifically from a variegated (and to some degree representative sample of CI/PSI Codes of Ethics. The chapter argues that there is an underlying connection between the principal tenets of moral philosophy and those of the interpreting profession (which mirror similar ethical principles in other professions. The tenet of Accuracy could be seen as a ‘contract’ between interpreter and client, interpreter and source, interpreter and text/translation process/profession. The chapter situates the interpreter’s sense of duty and responsibility towards this tenet at an individual and collective level, within the Kantian tradition of Duty. The other two tenets – impartiality and confidentiality – safeguard the interpreter’s conduct towards the source (author/speaker, professional community and institution as well as towards the receiver (reader/listener.

  2. Protective effect of anacardic acids from cashew (Anacardium occidentale) on ethanol-induced gastric damage in mice.

    Science.gov (United States)

    Morais, Talita C; Pinto, Natália B; Carvalho, Karine Maria M B; Rios, Jeison B; Ricardo, Nagila Maria P S; Trevisan, Maria Teresa S; Rao, Vietla S; Santos, Flávia A

    2010-01-05

    Cashew nut-shell liquid and the contained anacardic acids (AAs) have been shown to possess antioxidant, lipoxygenase inhibitory, anti-Helicobacter pylori and antitumor properties. Despite these known effects, hitherto there were no published reports on their likely gastroprotective effects. The present study was designed to verify whether AAs afford gastroprotection against the ethanol-induced gastric damage and to examine the underlying mechanism(s). Gastric damage was induced by intragastric administration of 0.2mL of ethanol (96%). Mice in groups were pretreated orally with AAs (10, 30 and 100mg/kg), misoprostol (50 microg/kg), or vehicle (2% Tween 80 in saline, 10mL/kg), 45min before ethanol administration. They were sacrificed 30min later, the stomachs excised, and the mucosal lesion area (mm(2)) measured by planimetry. Gastroprotection was assessed in relation to inhibition of gastric lesion area. To study the gastroprotective mechanism(s), its relations to capsaicin-sensitive fibers, endogenous prostaglandins, nitric oxide and ATP-sensitive potassium channels were analysed. Treatments effects on ethanol-associated oxidative stress markers GSH, MDA, catalase, SOD, and total nitrate/nitrite levels as an index of NO were measured in gastric tissue. Besides, the effects of AAs on gastric secretory volume and total acidity were analysed in 4-h pylorus-ligated rat. AAs afforded a dose-related gastroprotection against the ethanol damage and further prevented the ethanol-induced changes in the levels of GSH, MDA, catalase, SOD and nitrate/nitrite. However, they failed to modify the gastric secretion or the total acidity. It was observed that the gastroprotection by AAs was greatly reduced in animals pretreated with capsazepine, indomethacin, l-NAME or glibenclamide. These results suggest that AAs afford gastroprotection principally through an antioxidant mechanism. Other complementary mechanisms include the activation of capsaicin-sensitive gastric afferents, stimulation of endogenous prostaglandins and nitric oxide, and opening of K(+)(ATP) channels. These combined effects are likely to be accompanied by an increase in gastric microcirculation.

  3. Léxico indígena de flora y fauna en tratados sobre las Indias Occidentales de autores andaluces

    Directory of Open Access Journals (Sweden)

    Montes R. María Emilia

    1999-06-01

    Full Text Available Alrededor de la celebración de los quinientos años del descubrimiento de América se fomentaron y publicaron importantes investigaciones en muchas áreas del conocimiento. En el campo de la lingüística aparecieron nuevas obras dedicadas al estudio de las lenguas amerindias y al español de América. Desde la Cátedra de Historia de la Lengua Española de la Universidad de Granada se impulsan desde entonces una serie de investigaciones que culminaron en tesis doctorales y que abordan fenómenos del español, visto desde las dos orillas.

     

  4. Experimental evaluation of anti-hyperglycemic and hypolipidemic effects of stevia rebaudiana, Anacardium occidentale on wistar rats

    Directory of Open Access Journals (Sweden)

    Brijesh K.

    2016-12-01

    Conclusions: The present experimental study is preliminary one elaborate study with larger sample size is required to come up with a more significant result. [Int J Basic Clin Pharmacol 2016; 5(6.000: 2463-2467

  5. Descentramiento de fuentes escritas occidentales: hipótesis desde el género y los procesos educativos.

    Directory of Open Access Journals (Sweden)

    Suzy Bermúdez Q.

    2005-01-01

    Full Text Available La escritura alfabética de origen judeocristiano, como cualquier otra herramienta de construcción y transmisión de conocimiento, forma parte de marcos socio-culturales y espacio-temporales específicos. En el caso de Occidente, por siglos, ha sido más utilizada en ámbitos masculinos hegemónicos. El artículo plantea que si bien los estudios feministas letrados, en disciplinas como la historia, han realizado invaluables aportes en la recuperación del ayer de las mujeres, es pertinente revisar las tecnologías a partir de las cuales se apoya su labor. Lo anterior, por cuanto tales tecnologías parecen estar tañidas por perspectivas androcéntricas y antropocéntricas. Argumenta igualmente que las fuentes no escritas no sólo son de importancia para las/os no letradas/os, sino para visibilizar, desde ópticas más femeninas y menos eurocéntricas, el cotidiano iletrado de los sectores hegemónicos.

  6. Occurrence of Aspergillus section Flavi and section Nigri and aflatoxins in raw cashew kernels (Anacardium occidentale L.) from Benin

    DEFF Research Database (Denmark)

    Lamboni, Yendouban; Frisvad, Jens Christian; Hell, Kerstin

    2016-01-01

    The objective of this study was to evaluate the presence of Aspergillus section Flavi and A. section Nigri in cashew nuts harvested in the Northern Guinea (NG) and Southern Sudanian (SS) agro-ecological zones of Benin. Also, the presence of aflatoxins was investigated. For detection of fungal...... contamination, a total of 100 kernels/sample (with disinfection) and 40 kernels/sample (without disinfection) were plated. Seventy samples from fourteen villages were used. Aflatoxins occurrence was analysed on 84 samples by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC...... predominant, in NG and SS zones (90.2% and 87.2%) respectively. When non disinfected kernels were plated, A. section Nigri was predominant in both NG and SS zones, with percentages of 89.7% and 93.4%, respectively. None of the 84 nuts samples were positive for natural occurrence of aflatoxins with a detection...

  7. Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3

    NARCIS (Netherlands)

    Reitsma, Marit; Bastiaan-Net, Shanna; Sforza, Stefano; Valk, Van Der J.P.M.; Gerth Van Wijk, Van Roy; Savelkoul, H.F.J.; Jong, De N.W.; Wichers, H.J.

    2016-01-01

    In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), West

  8. Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3.

    Science.gov (United States)

    Reitsma, Marit; Bastiaan-Net, Shanna; Sforza, Stefano; van der Valk, Johanna P M; van Gerth van Wijk, Roy; Savelkoul, Huub F J; de Jong, Nicolette W; Wichers, Harry J

    2016-02-10

    In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.

  9. Estudio Comparativo De Tres Modelos Occidentales De Códigos Deontológicos Para Odontólogos

    Directory of Open Access Journals (Sweden)

    IGNACIO MACPHERSON MAYOL

    2013-01-01

    Full Text Available Se ha realizado un análisis comparado de los códigos deontológicos de tres entidades oficiales compe-tentes en la ética profesional de la Odontología: Code of Ethics for Dentists in the European Union, elabo-rado por el Council of European Dentists (CED; Código Español de Ética y Deontología Dental, editado por el Consejo General de Colegios de Odontólogos y Estomatólogos de España (CGCOE; y Principles of Ethics and code of Professional Conduct, de la American Dental Association (ADA. El análisis de la estructuración de los códigos, permite descubrir los diferentes planteamientos que regulan la deontología profesional según la tradición ético-legislativa de la que proceden. Si bien existen elementos comunes inherentes a la cultura occidental, se observan matices en la fundamentación, en la disposición y en el enunciado de los artículos que permiten deducir los fundamentos éticos que subyacen en cada uno de los códigos, a la vez que reflejan los problemas reales con los que se encuentran los odontólogos en el ejercicio de su profesión.

  10. L’homme au risque du vêtement. Un indice d’humanité dans la culture occidentale

    OpenAIRE

    Bartholeyns, Gil

    2013-01-01

    – Où allons-nous ? dit-elle.Il ne répondit pas. Il la tirait. Elle était obligée de courir pour le suivre. Elle trottait maladroitement, animal entravé par ce qui la différenciait des animaux : ses talons, ses vêtements, sa petite taille. Il lui fit quitter la route, s’approcher de la clôture qu’il avait franchie, huit jours avant.– Attendez, dit-elle, la voix entrecoupée, la clôture… Je ne peux pas… Comme elle se courbait pour passer entre les fils de fer par-dessus lesquels il avait sauté, ...

  11. Enlaces geodésicos intercontinentales : investigación sobre los enlaces occidentales Europa-África

    OpenAIRE

    Verdú Vázquez, Amparo

    2007-01-01

    La Geodesia, como ciencia encargada de determinar la forma de la Tierra, siempre ha estado vinculada a la ingeniería civil. Este vínculo cobra aún más importancia cuando se trata de comunicar territorios entre continentes vecinos, como es el caso de la Península Ibérica y el Norte de África. La oportunidad del presente trabajo viene dada por el interés actual en el enlace fijo Europa-África a través del Estrecho de Gibraltar, que se viene estudiando de forma intermitente desde hace tiempo,...

  12. Patrones de asentamiento y distribucion de recursos agricolas en dos unidades politicas prehispanicas en los Llanos Occidentales de Venezuela

    National Research Council Canada - National Science Library

    Juan Carlos Vargas Ruíz

    2012-01-01

    ....). El objetivo de dicha evaluación ha sido someter a prueba los dos modelos de evolución social propuestos para explicar la naturaleza y desarrollo de las sociedades complejas prehispánicas barinesas...

  13. Integrazione di dati geodetici e geologici per lo studio dei processi di deformazione attiva in Sicilia Sud-Occidentale

    OpenAIRE

    2014-01-01

    This research is based on a multidisciplinary approach including geological, geodetic and geophysical analyses and provides the first evidence of active deformation in south-western Sicily, in an area that partially falls on the macroseismic zone of the Belice 1968 destructive earthquake sequence. Even though this is the strongest seismic event recorded in Western Sicily in historical times, the seismogenic source is still undefined. The study area includes the westernmost frontal seg...

  14. EKSPLORASI SPASIAL CENDAWAN TANAH PADA SEKITAR RHIZOSFER TANAMAN JAMBU METE (Anacardium occidentale L. DI KARANGASEM DAN BULELENG-BALI

    Directory of Open Access Journals (Sweden)

    Farah Meita Pratiwi

    2013-12-01

    Full Text Available Coconut is one of an important plant to fulfil human’s life needed. This study wasconducted to determine the benefits of coconut plant parts. The study was conducted in someareas of Denpasar and Badung from 27 January until 4 February, 2012. The method used in thisstudy is exploratory survey method by using a questionnaire and interviews. The results showedthat the mostly used parts of the plant are fruit part of 53% (35% bungkak water, bungkak meat12.9%, 4.7% shell root 2.3%, 22 % stems, and leaves as many as 23%. The coconut tree is usedas an upakara (31%, drugs (24%, construction (14%, consumer (13%, crafts (2%, fuel (8%,roofs (2%, broom (2%, and household items (4%.Keywords: Cocos nucifera L. etnobotany, exploration survey

  15. Salmonella spp. e antibiotico-resistenza in Mammiferi e Uccelli selvatici in Italia nord-occidentale dal 2002 al 2010

    OpenAIRE

    Velca Botti; Francine Valérie Navillod; Lorenzo Domenis; Riccardo Orusa; Erika Pepe; Serena Robetto; Cristina Guidetti

    2013-01-01

    La Salmonella è un importante agente patogeno responsabile di zoonosi di notevole rilevanza economica. In Europa la salmonellosi è la seconda infezione trasmessa per via alimentare, in Italia il microrganismo continua ad essere la causa più frequente di infezione alimentare. In Europa sono in atto molti piani di sorveglianza di Salmonella in animali da allevamento, tuttavia il monitoraggio del microrganismo in animali selvatici è effettuato solo occasionalmente. Lo studio ha avuto l'obiettivo...

  16. Décoloniser l’histoire occidentale : Les naissances politiques de l’anthropologie historique

    Directory of Open Access Journals (Sweden)

    Misgav Har-Peled

    2010-06-01

    Full Text Available On peut se demander pourquoi Jacques Le Goff a utilisé le terme d’anthropologie et non ceux d’ethnologie ou d’ethno-histoire qui auraient pu tout autant correspondre à sa démarche intellectuelle. Si l’on envisage la dimension politique du geste originaire de l’anthropologie historique, on constate que c’est un effort pour rendre l’histoire aux démunis de l’histoire, aux prétendus « sans histoire ». Comme pour Levi Strauss, faire de l’anthropologie, pour les historiens, c’était participer à l'effort de décolonisation et le faire porter sur les sciences sociales.One wonders why Jacques Le Goff has used the term anthropology instead of ethnology or ethno-history which could equally reflect his intellectual approach. If we consider the political dimension of the original gesture in historical anthropology, we see that it is an effort to give a history to the subjects who were considered without history. As for Levi Strauss for historians, anthropology was involved in the effort of decolonization and of integrating it in social sciences.Perché Jacques Le Goff ha utilizzato il termine di antropologia e non quello di etnologia o di etnostoria che avrebbero potuto parimenti corrispondere al suo approccio intellettuale. Laddove si consideri la dimensione politica del gesto originario della antropologia storica, si constaterà che si è trattato di uno sforzo per rendere la storia agli sprovvisti di storia, ai pretesi “senza storia”. Così come fu per Levi Strauss, fare dell’antropologia, per gli storici, significava partecipare allo sforzo di decolonizzazione e

  17. Emplazamiento hacia el ENE del manto de Los Reales (Alpujarrides occidentales: criterios estructurales y de petrofábrica

    Directory of Open Access Journals (Sweden)

    Tubía, J. M.

    1984-08-01

    Full Text Available Los Reales nappe rests over Ojén nappe to the North of Sierra Alpujata. The nappe contact zone shows a intense plastic deformation with mylonitic rocks, The deformation decreases progressively going away from the contacto The structural study of footwall and hangingwall roen permits to attribute this deformation to the Los Reales nappe emplacement over that of Ojén. The foliation and stretching lineation distributions show a WSW-ENE shear direction. The mylonite microstructural characteristics and the enstatite crystalline preferred orientations in the basal peridotites give a mear sense in according that, Los Reales nappe is displaced, in their present position, from the WSW towards the ENE.

    El manto de Los Reales descansa sobre el manto de Ojén, al norte de Sierra Alpujata. La zona de contacto entre mantos exhibe una intensa deformación plástica con rocas miloníticas; la deformación disminuye progresivamente al alejarse del contacto. El estudio estructural de las rocas infra y suprayacentes al contacto permite atribuir esta deformación al emplazamiento del manto de Los Reales sobre el de Ojén. , Las distribuciones de la foliación y de la lineación, de estiramiento indican una dirección de cizallamiento WSW-ENE. Las características microestructurales de las milonitas y las orientaciones preferentes cristalinas de la enstatita en las peridotitas basales proporcionan un sentido de cizallamiento tal que el manto de Los Reales se ha desplazado, en su posición actual, desde el WSW hacia el ENE.

  18. Anacardic Acid Isolated From Cashew Nut Shell (Anacardium occidentale Affects Methane and Other Products in the Rumen Fermentation

    Directory of Open Access Journals (Sweden)

    A. Saenab

    2017-08-01

    Full Text Available Biofat is a hexane extract containing several bioactive compounds with anacardic acid as the major compound. This study aimed to examine the effect of anacardic acid on rumen fermentation, especially methane and its degradation in the in vitro rumen fermentation. The study was arranged in a completely randomized block design. The treatments were control (substrate or complete feed, biofat (substrate + 0.75 uL/mL biofat, and anacardic acid (substrate + 0.75 uL/mL anacardic acid. Measured variables were total gas production, methane, pH, concentration of ammonia (NH3, dry matter degrability (DMD, organic matter degrability (OMD, and neutral detergent fiber degrability (NDFD in the rumen. The chromatogram GC-MS analysis results indicated that the anacardic acid isolation process of the biofat produced nearly pure isolate (99.44%, and significantly decreased the production of methane by 51.21% and 39.62%, respectively. Anacardic acid degradation pattern in the in vitro rumen test showed a shifting of retention factor (Rf value after anacardic acid being incubated with the degradation of anacardic acid occurred after 24 h of fermentation. In conclusion, anacardic acid isolated from biofat has a dominant role to reduce the in vitro methane production. Anacardic acid is very potential to be used as a methane reducing agent.

  19. Trophic relationships in demersal communities of Western Mediterraneo occidentale: case studies from coastal and deep-sea ecosystems

    OpenAIRE

    Fanelli, Emanuela

    2008-01-01

    327 pages.-- PhD Tesis carried out at the Universitá degli Studi di Viterbo "La Tuscia", Dipartimento di Ecologia e Sviluppo Sostenibile, at the Institut de Ciències del Mar (ICM-CSIC) and at the Laboratorio di Ecologia Marina, IAMC-CNR.

  20. Role of CD14 in a Mouse Model of Acute Lung Inflammation Induced by Different Lipopolysaccharide Chemotypes

    NARCIS (Netherlands)

    Anas, A.A.; Hovius, J.W.R.; van 't Veer, C.; van der Poll, T.; de Vos, A.F.

    2010-01-01

    Background: Recognition of lipopolysaccharide (LPS) is required for effective defense against invading gram-negative bacteria. Recently, in vitro studies revealed that CD14 is required for activation of the myeloid differentiation factor (MyD)88-dependent Toll-like receptor (TLR)4 signaling pathway

  1. A New Publicly Available Chemical Query Language, CSRML, to support Chemotype Representations for Application to Data-Mining and Modeling

    Science.gov (United States)

    A new XML-based query language, CSRML, has been developed for representing chemical substructures, molecules, reaction rules, and reactions. CSRML queries are capable of integrating additional forms of information beyond the simple substructure (e.g., SMARTS) or reaction transfor...

  2. Chemotyping of diverse Eucalyptus species grown in Egypt and antioxidant and antibacterial activities of its respective essential oils.

    Science.gov (United States)

    Salem, Mohamed Z M; Ashmawy, Nader A; Elansary, Hosam O; El-Settawy, Ahmed A

    2015-01-01

    The chemical composition of the essential oil from the leaves of Eucalyptus camaldulensis, Eucalyptus camaldulensis var. obtusa and Eucalyptus gomphocephala grown in northern Egypt was analysed by using GC-FID and GC-MS techniques. The antibacterial (agar disc diffusion and minimum inhibitory concentration methods) and antioxidant activities (2,2'-diphenypicrylhydrazyl) were examined. The main oils constituents were 1,8-cineole (21.75%), β-pinene (20.51%) and methyleugenol (6.10%) in E. camaldulensis; spathulenol (37.46%), p-cymene (17.20%) and crypton (8.88%) in E. gomphocephala; spathulenol (18.37%), p-cymene (19.38%) and crypton (16.91%) in E. camaldulensis var. obtusa. The essential oils from the leaves of Eucalyptus spp. exhibited considerable antibacterial activity against Gram-positive and Gram-negative bacteria. The values of total antioxidant activity were 70 ± 3.13%, 50 ± 3.34% and 84 ± 4.64% for E. camaldulensis, E. camaldulensis var. obtusa and E. gomphocephala, respectively. The highest antioxidant activity value of 84 ± 4.64% could be attributed to the high amount of spathulenol (37.46%).

  3. Phenotypic and chemotypic studies using Arabidopsis and yeast reveal that GHB converts to SSA and induce toxicity.

    Science.gov (United States)

    Mekonnen, Dereje Worku; Ludewig, Frank

    2016-07-01

    γ-Hydroxybutyric acid (GHB) is a naturally occurring compound. It is detected in organisms such as yeasts, plants and mammals. GHB is produced from the reduction of succinic semialdehyde (SSA) by the activity of GHB dehydrogenase. Arabidopsis genome contains two GHB dehydrogenase encoding genes. The accumulation of GHB in ssadh mutants led to the speculation that GHB is the cause of aberrant phenotypes. Conversely, the accumulation of GHB in Arabidopsis plants subjected to abiotic stresses was described as a way of avoiding SSA induced damage. To resolve these contrasting views on GHB, we examined the effect of exogenous GHB and SSA on the growth of yeast and Arabidopsis plants. GHB concentrations up to 1.5 mM didn't affect shoots of Arabidopsis plants; however, root growth was inhibited. In contrast, 0.3 mM SSA has severely affected the growth of plants. Treatment of yeast wild-type strain with 10 mM SSA and 10 mM GHB didn't affect the growth. However, the growth of yeast uga2 mutant was greatly inhibited by the same concentration of SSA, but not GHB. Metabolic analysis and enzyme activity assay on native gel showed that Arabidopsis, but not yeast, possesses a GHB dehydrogenase activity that converts GHB back to SSA. The enzymatic assay has also indicated the existence of an additional GHB dehydrogenase encoding gene(s) in Arabidopsis genome. Taken together, we conclude that GHB is less toxic than SSA. Its accumulation in ssadh mutants and during abiotic stresses is a response to avoid the SSA induced damage.

  4. Different geographical distributions of two chemotypes of Barbarea vulgaris that differ in resistance to insects and a pathogen.

    Science.gov (United States)

    Christensen, Stina; Heimes, Christine; Agerbirk, Niels; Kuzina, Vera; Olsen, Carl Erik; Hauser, Thure Pavlo

    2014-05-01

    The interactions of plants with herbivores and pathogens have been suggested to drive the evolution of resistances in plants and in some cases new lineages and taxa. However, such divergence may require reproductive isolation, e.g., in allopatry. In the crucifer Barbarea vulgaris, some plants are resistant to the flea beetle Phyllotreta nemorum, due to production of specific saponins, whereas others are susceptible. Resistant and susceptible plants additionally differ in resistance to the pathogen Albugo candida, content of glucosinolates, and leaf pubescence, and they are genetically strongly divergent and partially reproductively incompatible. This suggests that at some point they were separated for a considerable length of time. Previously, the insect susceptible P-type had been described only from Denmark, Sweden, and Estonia, whereas the resistant G-type is widely distributed in Western Europe. Here, we tested whether the two plant types have divergent geographical distributions and maintain their distinct trait associations throughout their range. The insect-susceptible type was found in Russia, the Baltics, and parts of Fennoscandia, but not in Central Europe. In contrast, the insect resistant type was found from Finland and westwards. Their different trait associations were consistent within the two ranges. We therefore suggest that the two plant types diverged in allopatry at some time in the past, and evolved different resistances in response to local antagonists. The two plant types probably maintain their distinctness due to a hybridization barrier. Thus, the present distributions of the two types may be shaped by both historical processes and current differential biotic selection.

  5. Heterogeneity of linalool chemotypes of Lippia alba (Mill. N.E.Br., based on clonal half-sib progenies

    Directory of Open Access Journals (Sweden)

    Marcos Ribeiro Bottignon

    2011-08-01

    Full Text Available Lippia alba (Mill. N.E.Br. is an aromatic and medicinal shrub native to the American continent. Despite its potential as a source of essential oil for the pharmaceutical and cosmetics industries, few selection and genetic improvement studies have been carried out. The aim of this study was to provide genetic information on this species for breeding programs, showing its selection potential, by investigating clonal half-sib progenies. The following characteristics were evaluated per plant: leaf dry mass (LDM, total dry mass (TDM, leaf yield (LY, essential oil yield (EOY and oil production (OP. Estimates were made for the several genetic parameters including absolute genetic gain at 30% selection intensity, correlations and relative contribution of additive and environmental effects to phenotypic correlation. Two experimental trials on 30 progenies were conducted: one in Campinas, state of São Paulo (SP, Brazil, with two harvests of the aerial part, and one in Monte Alegre do Sul, SP, Brazil, with only one harvest. The trials were conducted in a randomized block design consisting of subplots with three replications, each plot (progeny consisting of 8 to 15 clonally-replicated plants with subplot harvesting. Variations were detected between progenies and harvests, as well as progeny/harvest interactions in the split plot experiment. High heritability and genetic gains were obtained at both sites for LDM, TDM and OP. The lowest variations among progenies were obtained for LY and EOY, highlighting selection problems. Negative additive genetic correlations were obtained for EOY × LDM, EOY × TDM, LY × TDM and LY × LDM. Selection for LDM resulted in increased oil production per plant (OP, even where there was a negative correlation between LDM × EOY.

  6. Chemotype profiling to guide breeders and explore traditional selection of tropical root crops in vanuatu, South Pacific.

    Science.gov (United States)

    Champagne, Antoine; Legendre, Laurent; Lebot, Vincent

    2009-11-11

    The objectives of the present study were to characterize good-quality cultivars, identify relationships between local eating preferences and primary compound content, and reveal biofortification potential in tropical root crop species aroids, yams, cassava, and sweet potato. A core sample of about 500 cultivars was assembled to represent the widest agro-morphological diversity. Very high coefficients of variation were found within species for proteins, sugars, cellulose, and mineral contents, whereas starch exhibited the lowest variation. Starch content was negatively correlated with other primary compound contents. For the national dish in Vanuatu, consumers prefer cultivars with high starch content. In contrast, preferences for daily consumption of boiled or roasted tubers are linked to average starch content, indicating great potential for improving primary compounds. Interestingly, relationships between flesh color and requirements for the traditional dish were revealed, suggesting opportunities for biofortification. The data produced will assist breeders in adopting appropriate biofortification strategies.

  7. A knowledge-informed chemotype approach to mining the ToxCast/Tox21 chemical-data landscape (WC9)

    Science.gov (United States)

    ToxCast and Tox21 chemical libraries currently exceed 2000 and 8000 unique chemicals, respectively, and span a broad diversity of chemical use-types, functionality, and toxicity mechanism and endpoint space. These libraries function as mechanism probes across hundreds of high-th...

  8. En busca de independencia y productividad: cómo influyen las culturas occidentales en las explicaciones individuales y científicas del envejecimiento

    Directory of Open Access Journals (Sweden)

    Laura L. Carstensen

    2002-01-01

    Full Text Available El artículo analiza, y sobre todo, cuestiona la influencia de la cultura occidental ---de países industrializados- en expectativas culturales, teorías implícitas y estereotipos acerca del envejecimiento y la vejez; en las formas de vivir y envejecer; así como en la formulación de políticas públicas. Destaca el influjo de dicha cultura en la investigación y la literatura científicas, particularmente en ciencias sociales, al tiempo que plantea miradas alternativas respaldadas por investigaciones sobre el particular.

  9. Atlas international de la vitalite linguistique. Volume 2: L'Europe Occidentale = International Atlas of Language Vitality. Volume 2: Western Europe.

    Science.gov (United States)

    McConnell, Grant D., Ed.; Gendron, Jean-Denis, Ed.

    The atlas offers a cartography of language functions, quantitatively measured in vitality rates, for Western Europe. The atlas has three parts: vitality by language; vitality by domain; and vitality by country. Eighty-three minority languages are covered. Domains considered include: global; religion; schools; mass media; administration; courts;…

  10. Coupled study of radionuclides and stable lead isotopes in Western Mediterranean; Etude couplee des radionucleides et des isotopes stables du plomb en Mediterranee occidentale

    Energy Technology Data Exchange (ETDEWEB)

    Miralles, J

    2004-05-15

    The aim of this work is to identified an environmental deposit able to have stored the atmospheric signal over large time-scale leaning our investigations on lead stable isotopes ({sup 206}Pb, {sup 207}Pb, {sup 208}Pb) and radionuclide ({sup 210}Pb, {sup 137}Cs, {sup 239}Pu, {sup 240}Pu) analysis. Owing to prior studies on anthropogenic lead sources, emission intensity and sedimentary accumulation, we choose to investigate the marine sediments of the Western Mediterranean. In the Gulf of Lions, the sedimentary accumulation is 110 {+-} 7 {mu}g.cm{sup -2} high in good agreement with the atmospheric inventory estimate we made from salt marshes of Camargue (99 {mu}g.cm{sup -2}). The reconstructed lead accumulation through a modelling step coupling {sup 210}Pb and stable isotopes corroborates the regional anthropogenic emissions (Ferrand, 1996). Briefly, in this context of the marine sediments are a relevant proxy to study past lead atmospheric concentration over the last hundred years. In the Alboran Sea, the study area is less constrained and more complex in terms of climatic, meteorological and hydrological conditions. The sedimentary inventory is of 153 {+-} 47 {mu}g.cm{sup -2}, 1,5 higher than in the margin sediments of the Gulf of Lions. The analysis of aerosols, sediments and settling particles evidences a continuity between the atmospheric signal and the sedimentary record. In spite of this encouraging results, the knowledge of the Alboran system is still too restricted in order to unambiguously conclude on accuracy of deep marine sediments of this area to study past atmospheric fallouts. (author)

  11. Organización comunitaria de los yumanos occidentales: Una revisión etnográfica y prospecto arqueológico

    Directory of Open Access Journals (Sweden)

    Don Laylander

    1991-01-01

    Full Text Available Existe actualmente múltiples aspectos sobre la cultura de las sociedades aborígenes de Baja California antes del contacto europeo que se desconocen en gran medida. Uno de estos aspectos es la organización de la comunidad ¿Cómo podemos aproximarnos a esta información?El autor de este trabajo presenta de una forma crítica las experiencias de distintos etnógrafos de este siglo con respecto a este tema y en base al análisis cuidadoso de la información propone una hipótesis. Finalmente, demuestra que el uso de estudios arqueológicos es útil como apoyo en el conocimiento de este aspecto sociocultural de los pueblos indígenas.

  12. Análisis de subsidencia de la Cuenca de las Salinas, Sierras Pampeanas occidentales Subsidence analysis of the Las Salinas Basin, western Sierras Pampeanas

    Directory of Open Access Journals (Sweden)

    Edgardo A. Azeglio

    2008-06-01

    Full Text Available En el presente trabajo se ha evaluado la subsidencia total de la cuenca de Las Salinas mediante diagramas tiempo - profundidad, considerando la subsidencia inicial, por carga y termotectónica. También se calculó la velocidad de subsidencia interválica en función del tiempo geológico. El área en estudio se ubica aproximadamente entre los paralelos 31°17´S y 32°40´S y los meridianos 66°38´W y 67°48´W en las cercanías de la localidad de Marayes, en el sitio denominado cuenca de Las Salinas. Los diagramas tiempo - profundidad permitieron relacionar cambios en la velocidad de subsidencia de la cuenca durante el proceso distensivo del Mesozoico y el actualmente compresional a la cual esta sometida, obteniéndose valores concordantes con áreas circundantes. Como resultado del análisis se encontró que la velocidad de subsidencia inicial fue de 0,0017 mm/año en el Carbonífero, la que fue aumentando lentamente hasta alcanzar los 0,00875 mm/año en el Cretácico. Este incremento disminuye sensiblemente durante el Cretácico superior, para posteriormente incrementarse abruptamente en el Terciario (0,043 mm/año. Este régimen se mantiene durante el Plioceno a Mioceno, alcanzando valores promedios de 0,269 mm/año, culminando con valores de 0,0973 mm/año. De la comparación entre ambos diagramas de subsidencia se observan distintos ritmos de sedimentación entre el sector norte y sur de la cuenca que podría ser la consecuencia de un fallamiento en el basamento. La comparación de estos valores con los obtenidos en la cuenca de Beazley indican que si bien los valores de subsidencia inicial y ritmo de sedimentación fueron similares. El inicio de la subsidencia en la cuenca de Beazley, se produjo con posterioridad (en el Triásico. La componente termotectónica en ambas cuencas es similar a pesar que el gradiente térmico normal de la zona aumenta sensiblemente en dirección sur.In the present work the total subsidence of Las Salinas basin has been evaluated trough time-depth diagrams, were the initial subsidence by load and thermotectonics were analyzed. The subsidence interval velocity was also calculated in function of the geologic time. The area under study is limited by the geographic coordinates of 31°17´ and 32°40´S latitude and 66°38´ and 67°48´W longitude, in the neighborhood of Marayes locality, in Las Salinas basin. The time-depth diagrams discriminate velocity changes of the basin subsidence rate during the extensional tectonics, and the present day dominant compressional process. The calculated results are in agreement with other regional rates. The obtained rates were: An initial subsidence velocity of 0.0017 mm/year during the Carboniferous period. This velocity had a constant and slow increase during Triassic and early Cretaceous times reaching mean values of 0.00875 mm/year in this last stage. The subsidence rate sensibly decreases during the late Cretaceous, and most part of the Tertiary. Subsequently a sudden change is observed during Pliocene - Miocene times reaching average values of 0.269 mm/year, and ending with rates of 0.0973 mm/year. The time-depth diagrams also show different subsidence rates from the northern and the southern parts of the basin, which could be the consequence of active basement faults during sedimentation. The initial subsidence and sedimentation rates were similar when these results are compared with Beazley Basin data, but the beginning of subsidence in Beazley Basin was later (Triassic time. The thermotectonic subsidences in both basins are similar, although the normal geothermal gradient of the area increases sensibly to the south.

  13. Reconstitution de la morphogenèse Oligocène-Miocène des Alpes occidentales par une approche pluridisciplinaire

    OpenAIRE

    Jourdan, Sebastien,

    2012-01-01

    The aim of this dissertation was to use a multidisciplinary approach, combining petrologic, geochemical and geo-thermochronologic analyses, to reconstruct the topographic and exhumational evolution of the Western Alps during Oligocene and Early Miocene times, in relation to regional geodynamic events. Because the sedimentary record of this evolution is preserved in the foreland basins on the both sides of the Western Alps in France and Italy, this approach allows identifying sediment provenan...

  14. Comparative Effects of Some Medicinal Plants: Anacardium occidentale, Eucalyptus globulus, Psidium guajava, and Xylopia aethiopica Extracts in Alloxan-Induced Diabetic Male Wistar Albino Rats

    Directory of Open Access Journals (Sweden)

    Victor Eshu Okpashi

    2014-01-01

    Full Text Available Insulin therapy and oral antidiabetic agents/drugs used in the treatment of diabetes mellitus have not sufficiently proven to control hyperlipidemia, which is commonly associated with the diabetes mellitus. Again the hopes that traditional medicine and natural plants seem to trigger researchers in this area is yet to be discovered. This research was designed to compare the biochemical effects of some medicinal plants in alloxan-induced diabetic male Wistar rats using named plants that are best at lowering blood glucose and hyperlipidemia and ameliorating other complications of diabetes mellitus by methods of combined therapy. The results obtained showed 82% decrease in blood glucose concentration after the 10th hour to the fortieth hour. There was significant increase P0.05 recorded in the glutathione peroxidase activity of E. globulus (100 mg/kg when compared to the test groups of P. guajava (250 mg/kg and X. aethiopica (250 mg/kg. Catalase activity showed significant increase P0.05, there was no significant difference seen between test group and treated groups. Meanwhile, degree of significance was observed in other parameters analysed. The biochemical analysis conducted in this study showed positive result, attesting to facts from previous works. Though these individual plants extracts exhibited significant increase in amelorating diabetes complication and blood glucose control compared to glibenclamide, a synthetic antidiabetic drug. Greater performance was observed in the synergy groups. Therefore, a poly/combined formulation of these plants extracts yielded significant result as well as resolving some other complications associated with diabetics.

  15. Comparative Effects of Some Medicinal Plants: Anacardium occidentale, Eucalyptus globulus, Psidium guajava, and Xylopia aethiopica Extracts in Alloxan-Induced Diabetic Male Wistar Albino Rats.

    Science.gov (United States)

    Okpashi, Victor Eshu; Bayim, Bayim Peter-Robins; Obi-Abang, Margaret

    2014-01-01

    Insulin therapy and oral antidiabetic agents/drugs used in the treatment of diabetes mellitus have not sufficiently proven to control hyperlipidemia, which is commonly associated with the diabetes mellitus. Again the hopes that traditional medicine and natural plants seem to trigger researchers in this area is yet to be discovered. This research was designed to compare the biochemical effects of some medicinal plants in alloxan-induced diabetic male Wistar rats using named plants that are best at lowering blood glucose and hyperlipidemia and ameliorating other complications of diabetes mellitus by methods of combined therapy. The results obtained showed 82% decrease in blood glucose concentration after the 10th hour to the fortieth hour. There was significant increase P 0.05 recorded in the glutathione peroxidase activity of E. globulus (100 mg/kg) when compared to the test groups of P. guajava (250 mg/kg) and X. aethiopica (250 mg/kg). Catalase activity showed significant increase P 0.05, there was no significant difference seen between test group and treated groups. Meanwhile, degree of significance was observed in other parameters analysed. The biochemical analysis conducted in this study showed positive result, attesting to facts from previous works. Though these individual plants extracts exhibited significant increase in amelorating diabetes complication and blood glucose control compared to glibenclamide, a synthetic antidiabetic drug. Greater performance was observed in the synergy groups. Therefore, a poly/combined formulation of these plants extracts yielded significant result as well as resolving some other complications associated with diabetics.

  16. Les anophèles et la transmission du paludisme à Ambohimena, village de la marge occidentale des Hautes-Terres Malgaches

    Directory of Open Access Journals (Sweden)

    Rajaonarivelo V.

    2004-03-01

    Full Text Available Une étude a été menée dans le Moyen-Ouest de Madagascar à 940 mètres d'altitude dans le village d'Ambohimena. Ce village est situé en dehors de la zone d'aspersions intra-domiciliaires d'insecticides rémanents. Deux vecteurs, An. funestus Giles, 1900 et An. arabiensis Patton, 1905 y sont présents. An. funestus est abondant au cours de la saison chaude et humide, avec deux pics d'abondance en décembre et avril. L'endophagie d' An. funestus est faible (taux d'endophagie = 35,3 % . C'est une espèce endophile (Taux d'endophilie = 78 % et anthropophile (taux d'anthropophilie = 64 %. Son infectivité est faible (taux d'infectivité = 0,20 %. Le taux d'inoculation spécifique de Plasmodium falciparum par l'espèce An. funestus a été de moins de 10 piqûres par homme et par an. Cette espèce présente une capacité vectrice maximale à la fin du premier tour de riz (janvier alors que son abondance maximale est observée à la fin du deuxième tour (avril-mai An. arabiensis est abondant entre décembre et janvier et est lié à la présence des gîtes pluviaux favorables au développement larvaire. C'est une espèce exophage (taux d'endophagie = 27,5 % et zoophile (taux d'anthropophilie = 7,8 %. An. arabiensis jouerait un rôle secondaire dans la transmission du paludisme car la présence des plasmodies n'a pas été mise en évidence (effectif testé = 871. Dans ce village, la stabilité du paludisme est modérée ou intermédiaire ; plus proche de l'instabilité que de la stabilité avérée. Il est suggéré que la lutte antivectorielle soit étendue aux zones de transition stabilité-instabilité qui constitueraient un réservoir de vecteurs et de plasmodies, afin de prévenir l'émergence de nouvelles épidémies sur les Hautes-Terres.

  17. La ilustración del libro chino: de la xilografía tradicional a las nuevas técnicas occidentales

    OpenAIRE

    Chieh, Wang

    2016-01-01

    Desde el punto de vista temático, la investigación aborda la ilustración del libro chino de un modo general, si bien tratando la influencia entre las relaciones con los ámbitos literario, pictórico, cultural, social y técnico. A pesar de su vasta amplitud, se profundiza en las tipologías y características formales, estéticas y técnicas de la xilografía por antonomasia de la ilustración del libro chino. Dicha temática está plenamente justificada por los conocimientos y la producción ilustrativ...

  18. Acquisition d'aimantations thermorémanentes partielles successives par la pyrrhotite monodomaine lors du refroidissement de la Zone dauphinoise interne (Alpes occidentales, France)

    Science.gov (United States)

    Crouzet, Christian; Rochette, Pierre; Ménard, Gilles; Prévot, Michel

    1997-11-01

    The magnetization of the marls and marly limestones from the internal part of the Dauphinoise zone is carried by monoclinic pyrrhotite. Our experiments show the equality of blocking and unblocking temperatures which suggests that magnetic grains are single domain. Thermal demagnetization analysis shows the presence of colinear components of opposite polarity. They are interpreted as partial thermoremanent magnetizations acquired during different polarity periods of the geomagnetic field at the time of the slow cooling of the studied area.

  19. Actualización en el límite entre sierras Pampeanas occidentales y Precordillera Oriental, en la provincia de San Juan

    Directory of Open Access Journals (Sweden)

    Juvenal J. Zambrano

    2008-03-01

    Full Text Available En este trabajo se analiza el sistema de fallamiento existente en la depresión tectónica de Tulum, ubicada en la parte este y sur de la provincia de San Juan, en el oeste árido de Argentina. El límite entre los dos sistemas morfoestructurales, uno la Precordillera Oriental y el otro el Sistema Pampeano Occidental ha sido establecido en trabajos anteriores, a lo largo del sistema de fallamiento de Tulum, que cruza a la depresión diagonalmente en dirección NNE. No obstante la disponibilidad de nueva información de sondeos eléctricos verticales y de algunas perforaciones para búsqueda de agua subterránea, efectuados al sur y al oeste del cerro Valdivia, permite señalar que hacia el suroeste de este núcleo de basamento metamórfico limitado en ambos flancos por fallas, el límite Sistema Pampeano y Precordillera se aproxima al cordón montañoso de sierra Chica de Zonda, continuando al sur en el cerro Salinas. De allí al SSO existen, en superficie, indicaciones de que el sistema de fallamiento continúa hasta el Montecito, donde el sistema pampeano de fallas queda interrumpido por la gran falla con actividad cuaternaria que sirve de límite oriental a la sierra de Las Peñas. El contacto entre ambos sistemas forma el extremo sur de la Precordillera Oriental en subsuelo. Este conocimiento estructural permite no solo ser aplicado a la prospección de agua sino también a la de hidrocarburos en la región.

  20. Meningo-encefalite equina da Halicephalobus gingivalis: contributo casistico nell’ambito delle attività di sorveglianza della Febbre del Nilo occidentale (West Nile disease

    Directory of Open Access Journals (Sweden)

    Gabriella Di Francesco

    2012-12-01

    Full Text Available Un cavallo di 7 anni è stato abbattuto dopo aver manifestato una grave sindrome neurologica a rapida evoluzione. Campioni tessutali sono stati inviati al Centro Studi Malattie Esotiche dell’Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale” (Istituto G. Caporale per gli accertamenti diagnostici del caso. Gli esami per le più comuni virosi neurologiche equine non hanno evidenziato la presenza di infezioni in atto. Istologicamente, si è osservata a livello encefalico la presenza di manicotti perivascolari e numerosi corpi parassitari, morfologicamente riferibili a Halicephalobus gingivalis. Il rinvenimento ha consentito di formulare la diagnosi di meningo-encefalite da H. gingivalis. Il caso riportato conferma che le encefaliti parassitarie devono essere annoverate nella diagnosi differenziale delle encefalopatie equine e sottolinea l’utilità dell’approccio diagnostico multidisciplinare.

  1. Frontera política y fronteras de colonización: las márgenes occidentales de la Cuenca de Maracaibo

    Directory of Open Access Journals (Sweden)

    Didier Ramousse

    1985-05-01

    Full Text Available El objeto de estudio se ubica en los límites entre Venezuela y Colombia, los cuales han permanecido durante mucho tiempo confusos y ciertas zonas en el golfo de Venezuela fueron siempre el objeto de discusiones. Estos dos países comparten una zona fronteriza de 250 kilómetros, la cual es una frontera de colonización y política entremezcladas donde los fenómenos tienen significación geopolítica, la cual se revela con bastante claridad a través de las tensiones existentes entre los dos estados. Hasta el momento los propósitos realizados para eliminar las posibilidades de conflicto no han encontrado eco, o bien han contribuido a aumentar el debate. Se ponen de relieve los mecanismos de ese conflicto fronterizo y sus repercusiones sobre la población y la puesta en valor con respecto a las regiones estudiadas.

  2. Copépodes, Cladocères et Rotifères du lac Sidi M'hamed Benali (Algérie Nord-Occidentale

    Directory of Open Access Journals (Sweden)

    Mohamed Ali Bouzidi

    2010-03-01

    Full Text Available En 2008, 33 espèces de Cladocères, Copépodes et Rotifères ont été observées dans le lac Sidi M'hamed Benali (contre 12 seulement en 1992.Les Copépodes dominent en mars, avril, juillet, août et septembre, avec respectivement des dominances (Do égales à : 56,6 %, 55,0 %, 55,0 %, 46,4 % et 90,0 %. Les Cladocères dominent en février (Do = 37,2 %, mai (Do = 47,4 % et décembre (Do = 48,6 %. Les Rotifères sont extrêmement abondants et présentent une large dominance en janvier (Do = 95,3 %. La population zooplanctonique est très faible en juillet et en août. Au cours de l'année le peuplement varie en fonction de la température, des conditions hydrologiques, de l'abondance de la nourriture et de la prédation par les poissons.In 2008, 33 species of Cladocers, Copepods and Rotifers have been observed in Sidi M’hamed Benali Lake (against only 12 species in 1992. The Copepods dominate in March, April, July, August and September, respectively with dominance (Do equal to 56.6 %, 55.0 %, 55.0 %, 46.4 % and 90.0 %. Cladocers dominated in February (Do = 37.2 %, May (Do = 47.4 % and December (Do = 48.6 %. The rotifers are extremely abundant and have a wide dominance in January (Do = 95.3%. The zooplankton population is very low in July and August. During the year, the population varies with temperature, water conditions, abundance of food and predation by fishes.

  3. UN ENCLAVE SOLUTRENSE EN LAS CORDILLERAS BÉTICAS OCCIDENTALES: LA CUEVA DEL HIGUERAL-GUARDIA (CORTES DE LA FRONTERA MÁLAGA, ESPAÑA

    Directory of Open Access Journals (Sweden)

    Concepción Torres Navas

    2013-11-01

    Full Text Available En el verano de 2011 se iniciaron trabajos de sondeo en la cueva del Higueral-Guardia (Málaga, España. Los sondeos han determinado la existencia de una importante secuencia con niveles del Paleolítico Superior y Medio todavía en estudio. No obstante, la actividad perpetrada por los clandestinos en la cueva, ha limitado significativamente las posibilidades de interpretación de los niveles solutrenses. En este trabajo pretendemos, además de realizar una presentación preliminar de los datos obtenidos, establecer una discusión sobre el valor que este tipo de registros puede tener de cara a establecer interpretaciones de rango mayor. Aspectos como la intensidad de la ocupación, la diacronía del registro, o la funcionalidad del sitio son discutidos en este sentido.In the summer of 2011 several test pits were made in the Higueral-Guardia Cave (Málaga, Spain. The sondages have determined the existence of an important Upper and Middle Paleolithic archaeological sequence, still under study. However, the illegal digging activity in the cave have significantly limited the possibilities of interpretation of the Solutrean levels. In this paper we present some preliminary results of the field work, and at the same time, establish a discussion about the value of such records in order to establish deeper anthropological interpretations. Aspects such as the intensity of the occupation, the diachronic value of the archaeological record, or the functionality of the site are discussed.

  4. Le recensement des plantes vasculaires et les originalités du peuplement végétal des monts Loma en Sierra Leone (Afrique Occidentale

    Directory of Open Access Journals (Sweden)

    P. Jaeger

    1983-12-01

    Full Text Available A FLOR1STIC INVENTORY OF THE LOMA MOUNTAINS IN SIERRA LEONE Our surveys in Sierra Leone of the flora of the Loma Mountains and the foothill country have enabled us to produce an inventory of the I 576 species, subspecies or varieties of Angiosperms belonging to 757 genera and 135 families. Gymnosperms are lacking. As in any other natural region, the Loma flora is the result of a long and complex process which took place in much earlier times. Under the influence of climatic factors, migration, speciation and also anthropological action, floristic elements with very different origins have ultimately arranged themselves into a particularly diversified but unstable mosaic and it is for us to unravel the mechanisms that have brought this about.

  5. En busca de independencia y productividad: cómo influyen las culturas occidentales en las explicaciones individuales y científicas del envejecimiento

    Directory of Open Access Journals (Sweden)

    Cara J. Rice

    2002-01-01

    Full Text Available El artículo analiza, y sobre todo, cuestiona la influencia de la cultura occidental ---de países industrializados- en expectativas culturales, teorías implícitas y estereotipos acerca del envejecimiento y la vejez; en las formas de vivir y envejecer; así como en la formulación de políticas públicas. Destaca el influjo de dicha cultura en la investigación y la literatura científicas, particularmente en ciencias sociales, al tiempo que plantea miradas alternativas respaldadas por investigaciones sobre el particular.

  6. Aux sources de l’herméneutique occidentale :les premiers commentaires dans les traditions grecque, juive et chrétienne

    Directory of Open Access Journals (Sweden)

    Christophe Rico

    2003-09-01

    Full Text Available L’article nuance, à propos de l’Antiquité, la distinction établie par Michel Charles entre rhétorique, entendue au sens de « production littéraire », et commentaire : dans le premier cas une œuvre littéraire constitue un modèle d’écriture que l’on peut imiter et parfaire ; dans le second, elle est transmise comme un monument intouchable que l’on peut citer et commenter, mais non pas imiter.Dans les traditions grecque, juive et chrétienne, le commentaire, en effet, doit plutôt être considéré comme une œuvre littéraire au second degré dans la mesure où il se fonde sur un texte commenté qui l’engendre et le suscite. L’article évoque dans cette perspective l’herméneutique du mot dans l’école d’Alexandrie, l’herméneutique de l’énoncé dans les écoles de philosophie antiques, les commentaires juifs anciens et médiévaux, l’herméneutique chrétienne ancienne, et leurs variations autour du sens littéral et du sens spirituel.

  7. Il sistema delle orchestre e dei cori giovanili di Abreu nel contesto occidentale. Un tentativo di dare significato pedagogico al trapianto dell’esperienza venezuelana

    Directory of Open Access Journals (Sweden)

    Daniele Branca

    2015-05-01

    Full Text Available The System of Children and Youth Choirs and Orchestras founded forty years ago by Josè Antonio Abreu in Venezuela, has become a significant alternative to the social ills faced by children and young people of the most disadvantaged neighborhoods of the South American metropolis, not only drugs and crime, but also the nihilistic logic that lacks all hope. It makes sense to transfer this experience in the wealthy West where the discomfort takes different forms? The answer is positive: music made together is ideal for sharing basic social values, construction of meaning and important experiences. The group is a perfect example of positive interdependence, commitment and responsibility, emblem of the community, micro-ideal society. Tolerance, inclusion, shared goals: values that can be a solution as well for our ills (bullying, eating disorders, depression, immaturity, inability to decision and commitment, dependence on families etc.. The System of Abreu is the credible alternative to the traditional way of teaching music, cold and elitist, and it stresses the importance of teachers in their role of great responsibility, both pedagogical and psychological. 

  8. Le paysage en peinture: espace rural et culture esthétique en Europe occidentale (XVIe - XVIIIe siècle

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    Jean BARROT

    1997-06-01

    Full Text Available L'invention de la perspective autorise à porter un regard de géographe sur les tableaux du XVIe au XVIIIe siècle. Le souci de réalisme permet de saisir des évolutions, selon le temps et les lieux, dans le paysage rural. Mais les choix de représentation traduisent bien davantage le contenu idéologique du regard que porte la clientèle du peintre sur la campagne qui reste dans cette période la base productive essentielle de la société.

  9. Clarificación combinada y evaluación sensorial de jugo de marañón (Anacardium occidentale L.

    Directory of Open Access Journals (Sweden)

    Jorge Osorio M.

    2013-10-01

    Full Text Available Objetivo. Obtener jugo clarificado de marañón, evaluar sensorialmente jugos clarificados optimizados y establecer diferencias químicas entre el jugo integral y el clarificado. Materiales y métodos. Los pseudofrutos se separaron manualmente de la nuez, fueron seleccionados y lavados, luego se realizó escaldado y extracción del jugo. El jugo integral y el de mayor aceptación fueron caracterizados químicamente. Los jugos se evaluaron sensorialmente (aceptación con una escala hedónica de 9 puntos y una prueba de ordenamiento por 30 catadores. Se empleó un diseño factorial de tres niveles combinado con la metodología de superficie de respuesta; las características químicas de los jugos se analizaron por prueba de homogeneidad de varianzas de Levene y la prueba T-Student de comparación de medias para muestras independientes. Resultados. Los jugos evaluados sensorialmente no presentaron diferencias estadísticas significativas entre sí (p≤0.05, p≤0.01; sin embargo el mayor porcentaje de aceptación fue del tratamiento 0.20% p/v Rapidasa® CX y 14.27 horas a 30ºC, se presentaron diferencias estadísticas significativas para las variables pH, °Brix, azúcares reductores y ácido ascórbico (p≤0.05; además, el contenido de ácido ascórbico se redujo notablemente en un 41.01% con respecto al jugo inicial. Conclusiones. El jugo clarificado con alto contenido de vitamina C, obtenido por tratamiento enzimático, constituye una forma de aprovechamiento agroindustrial del pseudofruto, teniendo aceptación para su consumo y sin presentar astringencia, con buen sabor, aroma, mediante tratamiento 0.20% p/v Rapidasa® CX y 14.27 horas a 30ºC.

  10. In vitro bioaccessibility of copper, iron, zinc and antioxidant compounds of whole cashew apple juice and cashew apple fibre (Anacardium occidentale L.) following simulated gastro-intestinal digestion.

    Science.gov (United States)

    de Lima, Ana Cristina Silva; Soares, Denise Josino; da Silva, Larissa Morais Ribeiro; de Figueiredo, Raimundo Wilane; de Sousa, Paulo Henrique Machado; de Abreu Menezes, Eveline

    2014-10-15

    Considering the lack of research studies about nutrients' bioaccessibility in cashew apple, in this study the whole cashew apple juice and the cashew apple fibre were submitted to simulated in vitro gastrointestinal digestion. The samples were analysed before and after digestion and had their copper, iron, zinc, ascorbic acid, total extractable phenols and total antioxidant activity assessed. As a result, for the whole cashew apple juice, the content of copper and iron minerals bioaccessible fraction were 15% and 11.5% and for zinc this level was 3.7%. Regarding the cashew apple fibre, the bioaccessible fraction for these minerals was lower than 5%. The ascorbic acid, total extractable polyphenols and total antioxidant activity bioaccessible fraction for whole cashew apple juice showed bioaccessibility percentages of 26.2%, 39% and 27%, respectively, while for the cashew apple fibre, low bioaccessibles levels were found. The bioacessible percentage of zinc, ascorbic acid and total extractable polyphenols were higher in cashew apple juice than cashew apple fibre. Copyright © 2014 Elsevier Ltd. All rights reserved.

  11. Atividades antioxidante e hepatoprotetora da polpa de caju (Anacardium occidentale L.) e Ãcidos anacÃrdicos em resposta ao estresse induzido por paracetamol

    OpenAIRE

    Caroline Nunes de Almada

    2013-01-01

    Many substances have been tested for their ability to protect against paracetamol toxicity and those that possess antioxidant properties have been the ones of major interest. The cashew tree is a tropical plant known for its biological properties related to its antioxidant potential. This work aims to evaluate the hepatoprotective effect of cashew apple pulp and anacardic acids in response to stress induced by paracetamol. Thus, were determined in the cashew apple pulp the levels of vitamin C...

  12. L'Education des enfants de travailleurs migrants en Europe occidentale (Bibliographie selective) (The Education of the Children of Migrant Workers in Western Europe [Selected Bibliography]).

    Science.gov (United States)

    Rosseel, Eddy

    This bibliography contains 322 entries covering a wide range of issues regarding the experience of West European countries in educating the children of foreign workers. Most of the entries are in languages other than English. (JB)

  13. Les casques Coolus-Mannheim de Vieille-Toulouse. Remarques sur les transferts d’armement a l’epoque republicaine dans les provinces occidentales

    Directory of Open Access Journals (Sweden)

    Gorgues, Alexis

    2005-12-01

    Full Text Available Not Available

    Se busca en este artículo el significado de los cascos de origen itálico que fueron encontrados en dos pozos de Vieille-Toulouse (Haute-Garonne, Francia, en niveles de mediados del siglo I.a.C. Se establece una posible relación entre estos elementos de panoplia y un texto del Bellum Gallicum (III, 20 en el que nobles de Tolosa son llamados por César para una campaña militar, en un marco de lazos personales de tipo clientelar.

  14. Effect of glycerine and essential oils (Anacardium occidentale and Ricinus communis on animal performance, feed efficiency and carcass characteristics of crossbred bulls finished in a feedlot system

    Directory of Open Access Journals (Sweden)

    Olga Teresa Barreto Cruz

    2014-11-01

    Full Text Available The effect of corn substitution by glycerine and essential oils on animal performance, apparent digestibility and red and white blood cells of crossbred bulls finished in feedlot was evaluated. Thirty bulls with average weight of 311±28.8 kg and 22±2 month-old were allocated in three diets: CON (without glycerine or essential oils, GLY (with glycerine and GEO (with glycerine and essential oils. The bulls were fed a diet of sorghum silage, cracked corn, soybean meal, urea, limestone and mineral salt. Three grams of cashew and castor oil/animal/day were included in GEO diet. Animals were kept in feedlot for 115 days and slaughtered at average weight of 467±40.6 kg. No differences (P<0.05 among diets regarding final body weight, average daily gain and feed conversion were reported. Ether extract intake was higher (P<0.05 in CON diet compared to the others. Dry matter, organic matter and crude protein digestibility was higher (P<0.05 in GLY diet compared to CON. Acid detergent fibre digestibility was higher (P<0.05 in CON compared to GLY diet. Nonfibrous carbohydrate, fibrous carbohydrate and ether extract digestibility were similar (P>0.05 among diets. No effect of glycerine and essential oil addition on total blood cholesterol, triglycerides, haemogram, leukogram and plasmatic proteins was observed. Corn replacement by glycerine and essential oils addition did not affect (P>0.05 carcass weight, dressing and conformation, carcass length and cushion thickness.

  15. Partenariat de recherche en Hautes écoles spécialisées Santé Travail social de Suisse occidentale

    Directory of Open Access Journals (Sweden)

    Kim Stroumza

    2011-10-01

    Full Text Available Au cœur des nouvelles politiques de la recherche tournée vers l’innovation, les Hautes écoles spécialisées suisses, récemment portées au niveau universitaire, ont pour mandat la recherche appliquée et le développement. Si la valorisation scientifique des travaux réalisés sous ce régime est visible dans les supports habituels, il n’en va pas de même de l’activité partenariale elle-même et des processus qui participent à sa constitution. L’analyse des narrations des protagonistes des partenariats permet de mettre en visibilité les phénomènes qui font la spécificité de cette activité, que les modèles de la diffusion sont impuissants à saisir. Prise entre pratique ordinaire, activité professionnelle et activité scientifique, soumise à la temporalité du processus, l’activité partenariale interroge la conception même du savoir qui sous-tend les modèles de recherche appliquée.Research partnership in the Swiss Universities of applied sciencesAt the heart of the new research policies tending towards innovation, the Swiss Universities of applied sciences, recently promoted to tertiary academic rank, have been given, as primary mission, to extend their activities in Applied Research and Development. If scientific valorization of this kind of research is visible in current media and publications, it is not so apparent for the partnership itself or the processes which constituted its development in the first place. The analysis of the narrations of the protagonists of the partnership shows the phenomenon which illustrates the specificity of this activity, that valorization models are unable to grasp. Caught between regular practice, professional and scientific activities, subject also to the temporality of the process, partnership activities question the very concept of the knowledge which underlines the models of applied research.Investigaciones conjuntas en Escuelas Superiores de Sanidad y Trabajo Social en Suiza occidental: compromiso de los agentes implicados y aprendizajes cruzadosEn el seno de las nuevas políticas de investigación orientadas hacia la innovación, las Escuelas Superiores especializadas suizas, recientemente incluidas en el ámbito universitario, tienen como objeto la investigación aplicada y el desarrollo. Si la valorización científica de los trabajos realizados dentro de este contexto es visible concretamente, no ocurre lo mismo por lo que respecta a la actividad conjunta y a los procesos que les dan forma. El análisis de las narraciones de los protagonistas pone visiblemente en evidencia los fenómenos que subrayan lo específico de este encaminamiento que los modelos de  difusión son incapaces de mostrar.  Estando entra la práctica habitual, la actividad profesional y la actividad científica, sometida está a la temporalidad el proceso, la actividad conjunta interroga la concepción misma de conocimientos que desembocan en métodos específicos de la investigación práctica.

  16. COMPOSITION AND BACTERICIDAL ACTIVITY AGAINST BENEFICIAL AND PATHOGENIC BACTERIA OF OREGANO ESSENTIAL OILS FROM FOUR CHEMOTYPES OF Origanum AND Lippia GENUS

    Directory of Open Access Journals (Sweden)

    L. Betancourt

    2012-01-01

    Full Text Available Diversos estudios demuestran la actividad antibacterial de distintos quimiotipos de aceites esenciales de orégano (OEO, carvacrol y timol, contra bacterias patógenas Gram positivas y Gram negativas, pero muy poca información está disponible sobre el efecto de los OEO en bacterias benéficas. El objetivo de este estudio fue comparar la composición y concentración mínima bactericida (CMB de OEO de quimiotipos del género Origanum: O. vulgare L. ssp. hirtum (OH, O. majorana (OM y O. vulgare L. (OL cultivados en Colombia, frente Lippia origanoides Kunth (LO nativo del Alto Pa-tía en Colombia y O. vulgare L. ssp. hirtum cultivado en Grecia (OG. Los OEO se ob-tuvieron por arrastre de vapor, la composición se determinó por cromatografía de gases acoplada a espectrometría de masas y la actividad antibacteriana mediante el método de dilución en caldo y cultivo en agar. Se evaluaron tres quimiotipos de OEO: OH y OG tipo-carvacrol, LO y OL tipo-timol y OM rico en compuestos sabinilo. Las variedades de orégano producidas bajo condiciones de invernadero a alta altitud mostraron un alto contenido de precursores. Un valor similar de concentración mínima bactericida contra Salmonella enteritidis (0.098 mg/mL fue observada para carvacrol, OH y LO. La más baja actividad bactericida contra las bacterias benéficas Lactobacillusacidophilusy Bifidobacterium breve fue observada para OM (6.25 mg/mL y LO (50 mg/mL, respectivamente. Estos resultados mostraron que LO, nativo de Colombia presentó un efecto antibacteriano comparable a OH y carvacrol contra enterobacterias patógenas y una baja actividad bactericida contra las bacterias benéficas. Estos resultados mostraron un efecto bactericida selectivo contra bacterias benéficas y patógenas de los quimiotiposOriganum ssp. y L. origanoides. Se comprobó un buen potencial de L. origanoides para uso como aditivo antimicrobiano para la salud humana y animal.

  17. Quantitative determination of seven chemical constituents and chemo-type differentiation of chamomiles using high-performance thin-layer chromatography.

    Science.gov (United States)

    Sagi, Satyanarayanaraju; Avula, Bharathi; Wang, Yan-Hong; Zhao, Jianping; Khan, Ikhlas A

    2014-10-01

    A simple and rapid high-performance thin-layer chromatographic method was developed for the separation and determination of six flavonoids (rutin, luteolin-7-O-β-glucoside, chamaemeloside, apigenin-7-O-β-glucoside, luteolin, apigenin) and one coumarin, umbelliferone from chamomile plant samples and dietary supplements. The separation was achieved on amino silica stationary phase using dichloromethane/acetonitrile/ethyl formate/glacial acetic acid/formic acid (11:2.5:3:1.25:1.25 v/v/v/v/v) as the mobile phase. The quantitation of each compound was carried out using densitometric reflection/absorption mode at their respective absorbance maxima after postchromatographic derivatization using natural products reagent (1% w/v methanolic solution of diphenylboric acid-β-ethylamino ester). The method was validated for specificity, limits of detection and quantification, precision (intra- and interday) and accuracy. The limits of detection and quantification were found to be in the range from 6-18 and 16-55 ng/band for six flavonoids and one coumarin, respectively. The intra- and interday precision was found to be 90%. The data acquired from high-performance thin-layer chromatography was processed by principal component analysis using XLSTAT statistical software. Application of principal component analysis and agglomerative hierarchial clustering was successfully able to differentiate two chamomiles (German and Roman) and Chrysanthemum. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. An Orally Active Phenylaminotetralin-Chemotype Serotonin 5-HT7 and 5-HT1A Receptor Partial Agonist that Corrects Motor Stereotypy in Mouse Models.

    Science.gov (United States)

    Canal, Clinton E; Felsing, Daniel E; Liu, Yue; Zhu, Wanying; Wood, JodiAnne T; Perry, Charles K; Vemula, Rajender; Booth, Raymond G

    2015-07-15

    Stereotypy (e.g., repetitive hand waving) is a key phenotype of autism spectrum disorder, Fragile X and Rett syndromes, and other neuropsychiatric disorders, and its severity correlates with cognitive and attention deficits. There are no effective treatments, however, for stereotypy. Perturbation of serotonin (5-HT) neurotransmission contributes to stereotypy, suggesting that distinct 5-HT receptors may be pharmacotherapeutic targets to treat stereotypy and related neuropsychiatric symptoms. For example, preclinical studies indicate that 5-HT7 receptor activation corrects deficits in mouse models of Fragile X and Rett syndromes, and clinical trials for autism are underway with buspirone, a 5-HT1A partial agonist with relevant affinity at 5-HT7 receptors. Herein, we report the synthesis, in vitro molecular pharmacology, behavioral pharmacology, and pharmacokinetic parameters in mice after subcutaneous and oral administration of (+)-5-(2'-fluorophenyl)-N,N-dimethyl-1,2,3,4-tetrahydronaphthalen-2-amine ((+)-5-FPT), a new, dual partial agonist targeting both 5-HT7 (Ki = 5.8 nM, EC50 = 34 nM) and 5-HT1A (Ki = 22 nM, EC50 = 40 nM) receptors. Three unique, heterogeneous mouse models were used to assess the efficacy of (+)-5-FPT to reduce stereotypy: idiopathic jumping in C58/J mice, repetitive body rotations in C57BL/6J mice treated with the NMDA antagonist, MK-801, and repetitive head twitching in C57BL/6J mice treated with the 5-HT2 agonist, DOI. Systemic (+)-5-FPT potently and efficaciously reduced or eliminated stereotypy in each of the mouse models without altering locomotor behavior on its own, and additional tests showed that (+)-5-FPT, at the highest behaviorally active dose tested, enhanced social interaction and did not cause behaviors indicative of serotonin syndrome. These data suggest that (+)-5-FPT is a promising medication for treating stereotypy in psychiatric disorders.

  19. Streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype I and emendation of the family Streptomycetaceae (Waksman and Henrici (1943)AL) emend. Rainey et al. 1997.

    Science.gov (United States)

    Kim, Seung Bum; Lonsdale, John; Seong, Chi-Nam; Goodfellow, Michael

    2003-01-01

    The taxonomic position of acidophilic actinomycetes selectively isolated from acidic soils and litter was examined using a polyphasic approach. The distinct 16S rDNA phyletic branch formed by representative strains was equated with related monophyletic clades that corresponded to the genera Kitasatospora and Streptomyces. The acidophilic isolates also exhibited a distinctive pH profile, a unique 16S rDNA signature, and contained major amounts of LL-diaminopimelic acid, galactose and rhamnose in whole-organism hydrolysates. It is proposed that these acidophilic actinomycetes be assigned to a new genus, Streptacidiphilus gen. nov., on the basis of genotypic and phenotypic differences. Three species were defined on the basis of DNA:DNA pairing and phenotypic data, namely, Streptacidiphilus albus sp. nov., the type species, Streptacidiphilus neutrinimicus sp. nov. and Streptacidiphilus carbonis sp. nov. Members of the genera Kitasatospora, Streptacidiphilus and Streptomyces share a number of key characteristics and form a stable monophyletic branch in the 16S rDNA tree. It is, therefore, proposed that the description of the family Streptomycetaceae be emended to account for properties shown by Kitasatospora and Streptacidiphilus species.

  20. Quantitative determination of phenolic compounds by UHPLC-UV-MS and use of principal component analysis to differentiate chemo-types of chamomile/chrysanthemum flowerheads

    Science.gov (United States)

    A new rapid UHPLC-UV-QTOF/MS method has been developed for the simultaneous analysis of nine phenolic compounds [cis-GMCA, chlorogenic acid, trans-GMCA, quercetagetin-7-O-ß-D-glucopyranoside, luteolin-7-O-ß-D-glucoside, apigenin-7-O- ß-Dglucoside, chamaemeloside, apigenin 7-O-(6"-O-acetyl-ß-D-glucop...

  1. Synthesis, biological evaluation, and structure-activity relationships for 5-[(E)-2-arylethenyl]-3-isoxazolecarboxylic acid alkyl ester derivatives as valuable antitubercular chemotypes.

    Science.gov (United States)

    Pieroni, Marco; Lilienkampf, Annamaria; Wan, Baojie; Wang, Yuehong; Franzblau, Scott G; Kozikowski, Alan P

    2009-10-22

    Tuberculosis (TB), mostly caused by Mycobacterium tuberculosis (Mtb), is one of the leading causes of death from infectious disease worldwide. Its coinfection with HIV and the emergence of multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) strains have further worsened the TB pandemic. Despite its global impact, TB is considered a neglected disease and no new anti-TB therapeutics have been introduced over the last four decades. The nonreplicating persistent form of TB (NRP-TB) is responsible for the length of the treatment and is the putative cause of treatment failure. Therefore, new anti-TB agents, which are active against both the replicating form of Mtb (R-TB) and NRP-TB, are urgently needed. Herein, we report the synthesis and structure-activity relationships (SAR) of a series of 5-[(E)-2-arylethenyl]-3-isoxazolecarboxylic acid alkyl esters as potent anti-TB agents. Several compounds had submicromolar minimum inhibitory concentrations (MIC) against R-TB and were active against NRP-TB in the low micromolar range, thus representing attractive lead compounds for the possible development of new anti-TB agents.

  2. 2种化学型迷迭香精油安全性分析%Safety Assessment of Two Chemotype Rosemary Essential Oils

    Institute of Scientific and Technical Information of China (English)

    吴亚妮; 王越; 黄健; 王瑞; 姚雷

    2010-01-01

    马鞭草烯酮型和1,8-桉叶油型2种化学型迷迭香(Rosmarinus officinalis L.)精油目前已有广泛的应用,但有若干精油成分疑存在安全性问题.本研究分别从急性经口毒性试验,急性皮肤刺激试验、多次皮肤刺激试验、眼刺激试验几个方面对其毒理和刺激性进行分析.以等比浓度灌胃法测定2种化学型精油对小鼠的急性毒性,采用改良寇氏法计算2种化学型精油对小鼠的半数致死量(LD50),实验结果表明,1,8-桉叶油型和马鞭草烯酮型迷迭香的LD50分别为7 220.35和8 088.24 mg·kg-1,LD50的95%可信限分别为5 172.53~8 124.41 mg·kg-1和6 982.70~9 842.84 mg·kg-1.马鞭草烯酮型和1,8-桉叶油型2种化学型迷迭香精油,在急性皮肤刺激试验中前者浓度在2.3%时,属于轻度刺激性,后者浓度在100%时属于中度刺激性;多次皮肤试验中,前者浓度在2.3%时属于轻度刺激性,后者浓度在40%时属于轻度刺激性.且相同浓度下前者刺激性要明显大于后者;眼刺激试验在50%的精油浓度范围内,2种化学型迷迭香精油强度显示为无刺激性.

  3. Quantification of kavalactones and determination of kava (Piper methysticum) chemotypes using near-infrared reflectance spectroscopy for quality control in vanuatu.

    Science.gov (United States)

    Lasme, Privat; Davrieux, Fabrice; Montet, Didier; Lebot, Vincent

    2008-07-09

    Kava ( Piper methysticum Forst f., Piperaceae) has anxiolytic properties and the ability to promote a state of relaxation without the loss of mental alertness. The rapid growth of the nutraceutical market between 1998 and 2000 has been stopped by a ban in Europe and Australia because of some suspicion of liver toxicity. It is now important to develop a fast, cheap, and reliable quality test to control kava exports. The aim of this study is to develop a calibration of the near-infrared reflectance spectroscopy (NIRS) using partial least-squares (PLS) regression. Two hundred thirty-six samples of kava roots, stumps, and basal stems were collected from the Vanuatu Agricultural Research and Technical Centre germplasm collection and from four villages. These samples, representing 45 different varieties, were analyzed using NIRS to record their absorption spectra between 400 and 2500 nm. A set of 101 selected samples was analyzed for their kavalactone content using HPLC. The results were used for PLS calibration of the NIRS. The NIRS prediction of the kavalactone content and the dry matter were in agreement with the HPLC results. There were good correlations between these two series of results, and coefficients ( R (2)) were all close to 1. The measurements were reproducible and had repeatability on par with the HPLC method. The NIRS system has been calibrated for the six major kavalactone content measurements, and it is suggested that this method could be used for quality control in Vanuatu.

  4. Estabilidade microbiológica, físico-química e sensorial de pedúnculos de caju (Anacardium occidentale L. processados por métodos combinados Microbiological, physico-chemical and sensorial stability of cashew apples (Anacardium occidentale L. processed by combined methods

    Directory of Open Access Journals (Sweden)

    Patricia Campos Mesquita

    2003-12-01

    Full Text Available Os pedúnculos de caju processados por métodos combinados e armazenados à temperatura ambiente (28ºC foram avaliados quanto à tendência a mudanças físico-químicas, microbiológicas e sensoriais. Os resultados confirmam que os tipos de obstáculos usados (redução da Aw, tratamento térmico brando, redução do pH, adição de ácido ascórbico, benzoato de sódio a 1000ppm e SO2 a 600 e 900ppm e sua intensidade foram capazes de assegurar a estabilidade microbiológica do produto durante a armazenagem à temperatura ambiente por 120 dias, bem como uma boa aceitação sensorial.Cashew apples processed by combined methods were stored at room temperature (28ºC in order to evaluate the tendency for chemical, microbiological and sensorial changes during 120 days of storage. Results confirmed that the obstacles used (reduction of water activity, mild heat treatment, pH reduction, ascorbic acid addition, 1000ppm sodium benzoate, 600 and 900ppm of SO2 and their intensities were capable to assure the microbiological stability and sensorial acceptance of the product during storage stability at room temperature for 120 days.

  5. OBTENÇÃO DE BEBIDA A PARTIR DE SUCO DE CAJU (Anacardium occidentale, L. E EXTRATO DE GUARANÁ (Paullinia cupana sorbilis Mart. Ducke OBTENTION OF DRINK FROM CASHEW APPLE JUICE (Anacardium occidentale, L. AND GUARANA EXTRACT (Paullinia cupana sorbilis Mart. Ducke

    Directory of Open Access Journals (Sweden)

    LILIANA COSTA SOARES

    2001-08-01

    Full Text Available A partir do extrato de semente de guaraná com 0,96% de cafeína, foram testadas várias formulações para obtenção de uma bebida, utilizando-se de suco de caju clarificado e concentrado, caramelo, aromatizantes artificiais de caju e guaraná e acidulante, tendo também como variável os diversos graus de doçura (ºBrix. As melhores formulações foram selecionadas de acordo com os resultados da avaliação sensorial, onde se utilizou o teste de ordenação. A formulação selecionada foi processada e envasada em embalagens de vidro. Para determinar a aceitabilidade, o produto final foi submetido a análises físico-químicas e sensoriais.From Guaraná seeds extract with 0,96% caffein content several formulations were evaluated in order to obtain a beverage using clarified cashew juice concentrate, caramel and guaraná artificial flavors and acidulant, using several sweetness grade a variable. The best formulations were selected according to sensorial evaluation results, using the ordenation test. The selected product (14 ºBrix formulation was manufactured and filled in glass bottles. In order to determine the acceptability the final product was submited to physical chemical and sensorial analysis.

  6. Avaliação da atividade antioxidante dos compostos fenólicos naturalmente presentes em subprodutos do pseudofruto de caju (Anacardium occidentale L. Evaluation of the Antioxidant Activity of Phenolic Compounds Naturally Contained in By-products of the Cashew Apple (Anacardium occidentale L.

    Directory of Open Access Journals (Sweden)

    Priscila Regina Bolelli Broinizi

    2007-12-01

    Full Text Available O presente trabalho teve como proposta avaliar a capacidade antioxidante do bagaço e do extrato bruto concentrado (EBC do pedúnculo de caju, tendo em vista o seu aproveitamento. O potencial antioxidante dos extratos aquoso (EAq e alcoólico (EAlc e das frações de ácidos fenólicos livres (AFL e esterificadas (solúvel AFS e insolúvel AFI desses subprodutos do pedúnculo de caju clone CCP-76 foi avaliado em sistema beta-caroteno/ácido linoléico, pelo teste de varredura de radical livre [2,2 difenil-1-pricril-hidrazil (DPPH•] e de Rancimat. Além do mais, o conteúdo de fenólicos totais e o perfil de ácidos fenólicos foram determinados usando-se o reagente de Folin-Ciocateau e por cromatografia gasosa, respectivamente. O EAq e a fração AFL dos subprodutos apresentaram o maior conteúdo de fenólicos. As frações de ácidos fenólicos exibiram expressiva atividade antioxidante, superior aos extratos estudados nos sistemas beta-caroteno e DPPH. Entretanto no teste Rancimat, os extratos apresentaram maior proteção à oxidação em relação às frações e ao BHT. Nas frações foram identificados os ácidos gálico, ferúlico, caféico, protocatecuico, quínico, cinâmico, gentíssico, p-cumárico e salicílico, os quais lhes conferem o potencial antioxidante. Estes resultados caracterizaram in vitro o potencial antioxidante do bagaço e do EBC do pedúnculo de caju clone CCP-76.This study aimed to determine the antioxidant capacity of cashew apple pulp and raw concentrated extract. The antioxidant potential of aqueous (EAq and alcoholic (EAlc extracts and of free phenolic acids (FPA and esterified (soluble - SPA and insoluble - IPA fractions of the by-products of the cashew apple clone CCP-76 were evaluated in a beta-carotene-linoleate system by the free radical [2,2-diphenyl-1-picrylhydrazyl (DPPH•] scavenging assay and the Rancimat test. In addition, the total phenolic content and phenolic acid profile were determined using Folin-Ciocateau reagent and gas chromatography, respectively. The EAq and the FPA fraction of the by-products showed the highest phenolic content. The phenolic acid fractions generally displayed stronger antioxidant activity than the extracts tested with the beta-carotene-linoleate and DPPH systems. However, in the Rancimat test, the extracts showed higher antioxidant activity than the fractions and BHT. Nine phenolic acids (gallic, ferulic, caffeic, protocatechuic, quinic, cinnamic, gentisic, p-coumaric and salicylic acids, which give the fractions their antioxidant potential, were identified and quantified (both free and esterified forms. These results characterized the in vitro antioxidant capacity of the pulp and raw concentrated extract of the cashew apple clone CCP-76.

  7. OBTENÇÃO DE BEBIDA A PARTIR DE SUCO DE CAJU (Anacardium occidentale, L.) E EXTRATO DE GUARANÁ (Paullinia cupana sorbilis Mart. Ducke) OBTENTION OF DRINK FROM CASHEW APPLE JUICE (Anacardium occidentale, L.) AND GUARANA EXTRACT (Paullinia cupana sorbilis Mart. Ducke)

    OpenAIRE

    LILIANA COSTA SOARES; GERARDO SÉRGIO FRANCELINO DE OLIVEIRA; GERALDO ARRAES MAIA; JOSÉ CARLOS SABINO MONTEIRO; ANTENOR SILVA JUNIOR

    2001-01-01

    A partir do extrato de semente de guaraná com 0,96% de cafeína, foram testadas várias formulações para obtenção de uma bebida, utilizando-se de suco de caju clarificado e concentrado, caramelo, aromatizantes artificiais de caju e guaraná e acidulante, tendo também como variável os diversos graus de doçura (ºBrix). As melhores formulações foram selecionadas de acordo com os resultados da avaliação sensorial, onde se utilizou o teste de ordenação. A formulação selecionada foi processada e envas...

  8. Site fidelity by bees drives pollination facilitation in sequentially blooming plant species.

    Science.gov (United States)

    Ogilvie, Jane E; Thomson, James D

    2016-06-01

    Plant species can influence the pollination and reproductive success of coflowering neighbors that share pollinators. Because some individual pollinators habitually forage in particular areas, it is also possible that plant species could influence the pollination of neighbors that bloom later. When flowers of a preferred forage plant decline in an area, site-fidelity may cause individual flower feeders to stay in an area and switch plant species rather than search for preferred plants in a new location. A newly blooming plant species may quickly inherit a set of visitors from a prior plant species, and therefore experience higher pollination success than it would in an area where the first species never bloomed. To test this, we manipulated the placement and timing of two plant species, Delphinium barbeyi and later-blooming Gentiana parryi. We recorded the responses of individually marked bumble bee pollinators. About 63% of marked individuals returned repeatedly to the same areas to forage on Delphinium. When Delphinium was experimentally taken out of bloom, most of those site-faithful individuals (78%) stayed and switched to Gentiana. Consequently, Gentiana flowers received more visits in areas where Delphinium had previously flowered, compared to areas where Delphinium was still flowering or never occurred. Gentiana stigmas received more pollen in areas where Delphinium disappeared than where it never bloomed, indicating that Delphinium increases the pollination of Gentiana when they are separated in time. Overall, we show that individual bumble bees are often site-faithful, causing one plant species to increase the pollination of another even when separated in time, which is a novel mechanism of pollination facilitation.

  9. Les glissements sous-marins de la pente continentale niçoise et la rupture de câbles en mer Ligure (Méditerranée occidentale).

    OpenAIRE

    Gennesseaux, Maurice; Mauffret, Alain; Pautot, Guy

    1980-01-01

    Le 16 octobre 1979, en mer Ligure, deux câbles sous-marins ont été détériorés par un courant de turbidité issu des abords de la côte niçoise. où de vastes glissements sous-marins non provoqués par un séisme se sont probablement produits le même jour. Les vitesses moyennes du courant, déterminées par l'heure d'un des glissements et les lieux et heures de rupture des câbles, ont été de 17 km/h en amont du premier câble et 7 km/h en aval, mais ont pu atteindre 40 km sur la pente continentale. [...

  10. Petrography and geochemistry of magmatic units from the western cordillera of Ecuador (0 deg. 30`S): tectonic implications; Petrographie et geochimie des unites magmatiques de la cordillere occidentale d`Equateur (0 deg. 30`S): implications tectoniques

    Energy Technology Data Exchange (ETDEWEB)

    Cosma, L.; Mamberti, M.; Gabriele, P. [Lausanne Univ. (Switzerland). Institut de Mineralogie et Petrographie; Cosma, L.; Lapierre, H.; Jaillard, E.; Mambert, M. [Universite Joseph-Fourier, UPRES A-CNRS 5025, Grenoble I, 38 (France). Institut Dolomieu; Jaillard, E.; Laubacher, G. [ORSTOM, 75 - Paris (France). Dept. RED; Bosch, D. [Montpellier-2 Univ., CNRS-UMR 5567, 34 (France); Desmet, A. [Universite Henri Poincare, 54 - Vandoeuvre-les-Nancy (France). Lab. de Petrologie

    1998-12-31

    The cost and western Cordillera of Ecuador are made of accreted oceanic terranes, separated from from the continental margin by a suture zone containing tectonic slices of mafic rocks. The western Cordillera contains three distinct magmatic units. Ultramafic and mafic cumulates from the suture zone (San Juan slice) represent likely the plutonic roots of oceanic plateau basalts. The mafic cumulates are LREE depleted and Ta and Pb enriched (primitive mantle). Their Nd and Pb isotopic compositions suggest that they derived from an enriched OIB type mantle source. Pre-Coniacian arc-tholeiites present flat REE patterns, low Pb and Th contents, and high {xi}{sub Nd(T=100Ma)} (+7.5 to + 7.9) which are indicative of their derivation from a mantle source. These arc-tholeiites developed likely in an intra-oceanic setting. The Eocene calc-alkaline lavas differ from the arc-tholeiites because they are LREE-enriched and have lower {xi}{sub Nd(T=50Ma)} ratios. Their high Pb and Th contents are probably related to crustal assimilation during the magmas ascent. Their Pb isotopic compositions support involvement of subducted pelagic sediments in their genesis. These lavas represent likely the remnants of a continental calc-alkaline magmatic arc. The continental-arc setting of the Eocene lavas demonstrates that these volcanic rocks postdate the accretion of the western Cordillera, upon which they rest unconformably. Therefore, the accretion of the western Cordillera may have occurred in late Paleocene times, as for part of the oceanic terranes of coastal Ecuador. Nevertheless, the occurrence of a collisional event during late Santonian-early Campanian times is strongly suggested by: the arrival of detrital quartz on oceanic series of the western Cordillera by Campanian-Maastrichtian times, a regional unconformity locally dates early Campanian, the arc-jump observed on coastal Ecuador in Santonian times, and finally a thermal event recognised in the eastern Cordillera around 85-80 Ma. (authors) 50 refs.

  11. Evolution de la région Baléares (Méditerranée occidentale) du Néogène à l' Actuel : aspects géodynamiques et paléoenvironnementaux.

    OpenAIRE

    Driussi, Olivier

    2014-01-01

    The western Mediterranean is a complex area which results from the interaction between orogenic and extensional processes, within the framework of the convergence between Eurasia and Africa. The Balearic promontory is part of this region and is located to the junction of the three main basins: Liguro-Provençal, Algerian (oceanic) and the Valencia Trough (aborted rift). The age and opening direction of the Algerian basin are still unknown and the structure of the Balearic Promontory, poorly co...

  12. La création de l'image des cultures non-occidentales: l'influence du manuel d'histoire sur l'opinion en Flandre durant la période 1945-1984

    Directory of Open Access Journals (Sweden)

    A. De Baets

    1988-01-01

    Full Text Available This Ph.D. study tries to answer the question "Do history textbooks have an impact on people's ideas about other cultures?", by comparing the contents of a large sample of influentialhistory textbooks and curricula (covering 1945-1984 with the results of a wide array of public opinion surveys about the Third World and immigrants (covering 1949-1987.The theoretical part reviews ethnocentrism, cultural relativism and racism as dimensions of cultural images, focuses attention on the phenomenon and mechanism of influence, andassesses the place of the textbook in the complex network of factors acting upon youngsters and adults, inside and outside the school.The methodological part discusses the value of the two sources (relevance, validity and reliability of surveys; availability and use of history textbooks; comparability of both. Universesof both sources are constructed and samples drawn from them. These samples are analysed with mutually attuned question batteries.The double empirical analysis leads to two series of conclusions and trends that are compared with each other. Five parallel trends are found in textbooks and surveys (dominantbut decreasing ethnocentrism; decreasing nationalism; absence of racism; poor awareness of other cultures; social-evolutionist thinking. They coincide in time, while, for textbooks tohave influence, trends there should precede these in the public. Four other trends only partially coincide, or diverge.In the case of still two other trends, mutual influence could be plausibly postulated.In globo, no firm evidence was found for the thesis that history textbooks autonomously influence the public. It rather is the general climate of opinion that, with years of delay, acts upon the textbook authors. The role of these authors, the immediate influences upon them (editors, curricula planners, academics, inspectors, teachers, parents and pupils, their biographies and their degree of representativeness vis-à-vis public opinion, are largely discussed.As a conclusion, the study argues that, by presenting cultural diversity more accurately, new history textbooks could become master cards in the construction of open and tolerant intercultural views and mentalities.

  13. Sismotectonique et déformation actuelle de la terminaison nord-occidentale de l'arc égéen (Iles ioniennes, Acarnanie, Epire, Grèce)

    OpenAIRE

    Amorese, Daniel

    1993-01-01

    La sismicité de la terminaison Nord-Ouest de l'Arc égéen est analysée à partir de données microsismiques. Celles-ci ont été recueillies par 52 stations qui ont fonctionné, en été 1989, sur les îles Ioniennes, en Acarnanie et en Epire. 656 microséismes ont été localisés et 128 solutions focales construites. La région étudiée constitue la zone de transition entre subduction océanique égéenne (au Sud de Céphalonie) et subduction continentale adriatique (au Nord de Zante). L'activité microsismiqu...

  14. «Approchez avec crainte de Dieu, foi et amour» : le programme iconographique de la travée occidentale de l’abside en Moldavie (XVe – XVIe siècles

    Directory of Open Access Journals (Sweden)

    Vlad Bedros

    2015-12-01

    Full Text Available This paper, informed by an inventory of the apse iconography in Moldavian monuments from the late 15th to the first half of the 16th century, draws attention to an overlooked element of the otherwise familiar iconographic system employed – with occasional nuances – within the Post-Byzantine tradition in the aftermath of the Alosis: the selection of themes to be displayed in the bay which links the apse to the naos. The arrangements encountered in monuments dating from (approx. 1490 to 1535 put forward a variety of formulas which – although described in a sequence that deliberately emphasizes the similarities which came across – are nonetheless indicative of a broad and not necessarily fluent use of the late Byzantine tradition. It seems that the basic message conveyed by these iconographic boundaries aims at stressing the holiness of the sanctuary (involving a separation from the iconography of the naos, in the cases of Probota and Saint-Georges in Suceava but also, in a manner which could be informed by the late Byzantine perception of the liturgy – as deduced from the writings of Nicolas Cabasilas and St. Simeon of Thessaloniki –, at joining together naos and apse, via ‘iconographic belts’ (most frequently the Passion cycle, which sets out from and ends up in the sanctuary. The abundance of theophanic themes invites one to consider this iconographic thresholds in relation with the receiving of Communion, administered on the solea which lies underneath.

  15. Uma crítica pedagógica dos sistemas educacionais ocidentais Crítica pedagógica de los sistemas educativos occidentales A pedagogical criticism of occidental educational systems

    Directory of Open Access Journals (Sweden)

    José María Quintana Cabanas

    2005-03-01

    Full Text Available Este artículo pretende mostrar la necesidad de una visión crítica de la educación actual y las diferentes reformas ocurridas en España. Enfoca los fenómenos que vienen interfiriendo en la educación, indicando como uno de los más alarmantes, la violencia escolar. Aborda los problemas enfatizando que la educación actual sufre del síndrome de la falta de control y de la desorientación, además de señalar el tema del fracaso escolar como un agravante de los principales problemas del sistema educativo.Este artigo pretende mostrar a necessidade de uma visão crítica da educação atual e as reformas ocorridas na Espanha. Enfoca os fenômenos que vem interferindo na educação, apontando como um dos mais alarmantes, a violência escolar. Aborda os problemas enfatizando que a educação atual adoece da síndrome da falta de controle e da desorientação, além de apontar o tema do fracasso escolar como um agravante dos principais problemas do sistema educacional.This articles points out the need of a current critical view of education and the different educational reforms that took place in Spain. It focus the phenomena that are interfering in the education process, mainly those related to school violence. It also emphasizes the lack of control and guidance, that places school failure as one of the most serious problems in the educational system.

  16. The plutonium as transfer tracer and particulates contribution accumulating from the Rhone to the north-western Mediterranean sea; Le plutonium comme traceur du transfert et de l`accumulation des apports particulaires du Rhone en Mediterranee nord-occidentale

    Energy Technology Data Exchange (ETDEWEB)

    Noel, M.H.

    1996-03-29

    To follow the alluvial deposits from the Rhone in Mediterranean Sea, since the principle hydroelectric constructions (1960), it was necessary to find an adequate tracer. The plutonium isotopes have satisfied to the different characteristics. The specificity of the Rhone contribution is bound to the existence of the irradiated spent fuels reprocessing plant of Marcoule; the releases of this plant are characterized by the predominance of Plutonium 238. The results of this study show that the percentage of sediments coming from the Rhone in the recent contribution, is low beyond the pro delta: less than 5% of the sediment of the continental shelf concern the Rhone contribution. However, there are two zones for which the contribution is important and could play a role in the particulate transfer towards the high sea. (N.C.). 157 refs., 60 figs., 50 tabs.

  17. Gisements naturels Pléistocenes et Holocènes des cavités des Pyrénées Occidentales Françaises: Inventaire et datages 14C.

    Directory of Open Access Journals (Sweden)

    Andre Clot

    1986-01-01

    Full Text Available Recientes descubrimientos fortuitos de espeleólogos permiten completar los datos proporcionados por los yacimientos paleontológicos clásicos. Se redacta un primer inventario de las cavidades de los Pirineos Atlánticos y de los Altos Pirineos franceses, donde han caído animales salvajes accidentalmente, sea en simas verticales o sea a lo largo de fuertes pendientes. Se da cuenta de dataciones 14 C sobre algunos de estos restos.

  18. Arte rupestre paleolítico y postpaleolítico al aire libre en los Montes de Toledo occidentales (Toledo, Castilla - La Mancha, España: noticia preliminar

    Directory of Open Access Journals (Sweden)

    Jesús F. JORDÁ PARDO

    2009-12-01

    Full Text Available RESUMEN: Se dan a conocer en este trabajo los grabados y pinturas rupestres localizados recientemente en el extremo occidental de los Montes de Toledo (comarca toledana de La Jara en la vertiente meridional de la Cuenca del Tajo. Una primera zona, situada en el valle del río Huso, se desarrolla al aire libre sobre afloramientos de pizarras y en ella se localizan al menos dos conjuntos rupestres: uno caracterizado por grabados de trazo fino atribuidos al Paleolítico Superior y otro realizado mediante diversas técnicas de grabado con una cronología muy amplia desde la Prehistoria reciente hasta épocas históricas. La segunda zona corresponde al valle del río Gévalo y por el momento corresponde a un único gran abrigo desarrollado en cuarcitas en cuyas paredes aparecen pinturas rojas atribuibles a la Prehistoria reciente sobre las que se superponen grabados de trazo fino y repiqueteado con elementos claramente prehistóricos y otros con un marcado carácter histórico.ABSTRACT: This paper deals with rock art findings recently located on the Western edge of Toledo Mountains at the Southern slope of Tagus Basin. The first finding área, placed in the valley of the Huso River, is an open air rock art site on shale outcrop where two main groups of patterns may be seen: fine - line engravings from Upper Palaeolithic times as well as several motifs using various engraving techniques rahging a wider time span from later prehistoric ages to historical times. The second área lies in the valley of the Gévalo River and is a large quartzite rock shelter containing on his walls red paintings dated on Postpalaeolithic times that placed under engravings made using fine-line and beating techbiques both from prehistórica! and historical ages.

  19. Western oil companies in the eighties and nineties: from multi-nationalization to globalization?; Les compagnies petrolieres occidentales dans les decennies quatre-vingt et quatre-vingt-dix: de la multinationalisation a la globalisation?

    Energy Technology Data Exchange (ETDEWEB)

    Bourgeois, B. [Grenoble-1 Univ., 38 (France)

    1997-12-31

    Ways and intensity of trans-nationalization process of Western oil companies have dramatically changed over the last thirty years. After a decline from 1970 to 1984, a new era of investment extraversion from home base is developing. We discuss then the extent to witch this new era is a part of a larger process of firms globalization interacting witch political internationalization. The existence of a clear globalization trend is recognized inside the oil industry, but with a persisting specificity coming from the access conditions to the oil deposits. (author)

  20. Actualización en el límite entre sierras Pampeanas occidentales y Precordillera Oriental, en la provincia de San Juan Updating the boundary between the western Pampean ranges and the eastern Precordillera, province of San Juan

    Directory of Open Access Journals (Sweden)

    Juvenal J. Zambrano

    2008-03-01

    Full Text Available En este trabajo se analiza el sistema de fallamiento existente en la depresión tectónica de Tulum, ubicada en la parte este y sur de la provincia de San Juan, en el oeste árido de Argentina. El límite entre los dos sistemas morfoestructurales, uno la Precordillera Oriental y el otro el Sistema Pampeano Occidental ha sido establecido en trabajos anteriores, a lo largo del sistema de fallamiento de Tulum, que cruza a la depresión diagonalmente en dirección NNE. No obstante la disponibilidad de nueva información de sondeos eléctricos verticales y de algunas perforaciones para búsqueda de agua subterránea, efectuados al sur y al oeste del cerro Valdivia, permite señalar que hacia el suroeste de este núcleo de basamento metamórfico limitado en ambos flancos por fallas, el límite Sistema Pampeano y Precordillera se aproxima al cordón montañoso de sierra Chica de Zonda, continuando al sur en el cerro Salinas. De allí al SSO existen, en superficie, indicaciones de que el sistema de fallamiento continúa hasta el Montecito, donde el sistema pampeano de fallas queda interrumpido por la gran falla con actividad cuaternaria que sirve de límite oriental a la sierra de Las Peñas. El contacto entre ambos sistemas forma el extremo sur de la Precordillera Oriental en subsuelo. Este conocimiento estructural permite no solo ser aplicado a la prospección de agua sino también a la de hidrocarburos en la región.An analysis is made of the fault system in the Tulum tectonic depression, situated in the southeastern part of the province of San Juan, which is in the arid western part of Argentina. The boundary of two morphostructural systems occurs in this valley: the eastern Andean Precordillera and the western Pampean ranges. In former studies it was found that this boundary runs along a fault system that crosses the Tulum depression with a NNE trends. The availability of new information from electric resistivity tests and wells drilled for ground water show that the metamorphic basement exposed in the Valdivia low, isolated mountain continues to the SSW thereof in the subsurface, as a structural high limited by faults on both flanks. Further south, the basement crops out again in the Salinas hill and continues in the shallow subsurface, as suggested by geomorphic features and geophysic and well data, along the same trend, as far as the Montecito. In this way, the Tulum fault system comes nearer the Precordillera and, at the Montecito hill, ends against the Las Peñas range, which is in the central Precordillera and is limited to the east by a fault of regional importance with Quaternary activity. The contact between the Pampean and Precordillera systems was found, then, to continue further south than shown in former studies and represents the surface and subsurface boundary of the Eastern Precordillera along all its length. This new structural information may be useful for exploring ground water and hydrocarbons.

  1. Explicación física de la consonancia y disonancia musical y su aplicación a las estructuras armónicas de las escalas occidentales y orientales

    OpenAIRE

    Fernández, Olaya

    2016-01-01

    Tesis doctoral This work is divided in two parts. At the first part we worked experimentally about the consonance and dissonance between different sounds. We are going to prove, if the theoretical and statistical experiments are true. We use a violin, clarinet and pipe organ for the emission of the sounds. At the second part we are going to prove that occidental and oriental musical scales are made by consonance intervals Universidad de Oviedo

  2. Scarabaeides, Buprestides et Cérambycides de l’Afrique occidentale, envoyés au Musée de Leyde par MM. Veth et van der Kellen

    NARCIS (Netherlands)

    Lansberge, van J.W.

    1886-01-01

    Modice convexus, subnitidus, niger vel aeneo-niger; capite granulato; thorace confertim granulato, postice remote punctato, basi angulata, lateribus subcrenulatis; scutello conspicuo, elongato; elytris subopacis, irregulariter obsolete punctatis, sutura elevata nitida; pectore antice acute tubercula

  3. Lutte contre les ravageurs des stocks de céréales et de légumineuses au Sénégal et en Afrique occidentale : synthèse bibliographique

    Directory of Open Access Journals (Sweden)

    Guèye, MT.

    2011-01-01

    Full Text Available Controlling pests of cereals and legumes in Senegal and West Africa: a review. Post-harvest losses of cereals and legumes are a major problem in Senegal and West Africa. The solutions to eliminate insects, major pests of stored products were mainly chemical. However, due to pollution associated with pesticides use, selection of resistant strains, environmental pollution, poisoning, the search for alternatives is needed. It is reported on different methods of protecting stocks performed alternatively or in combination with pesticides. The major pest species encountered, particularly Prostephanus truncatus (Horn, insect emerging in Senegal, could be controlled by alternative methods including specially the use of insecticide plants. Different aspects related to this alternative way to chemical pesticides are reviewed herein.

  4. Effects of the global changes on the aquatic ecosystems in West Europe - role of the plankton communities; Effets des changements globaux sur les ecosystemes aquatiques d'Europe Occidentale - role des communautes planctoniques

    Energy Technology Data Exchange (ETDEWEB)

    Souissi, S. [Universite des Sciences et Technologies de Lille, Ecosystem COmplexity REsearch Group, Station Marine de Wimereux, CNRS - FRE 2816 ELICO, 62 - Wimereux (France)

    2007-07-01

    Examination of long-term records of aquatic ecosystems has provided useful information to find out their major driving forces. Understanding the impact of climate change on these ecosystems, the management of their resources and the extrapolation between sites are the main scopes of actual and emerging studies. Such goals can be achieved by inter-site and inter-ecosystem comparisons. This approach was undertaken during our project which has the originality to tackle with marine and freshwater ecosystems. It allowed us to compile and validate several multi-decadal time series of planktonic and other physical driving forces at local and regional scales. Then, the same methodology based on the analysis of the variability of climate indices and biological data across several spatial scales was used. The different ecosystems analyzed here showed clear response to the North Atlantic climate variability. Although the local differences abrupt changes in community composition occurred in all ecosystems in the middle of the years 80. During this period there was also a major shift in climatic conditions during winter and early spring, suggesting an impact of climatic factors. Phenological changes were also observed in plankton communities in all sites. The consequences of the modifications of plankton dynamics on higher trophic levels were also showed. Fluctuations in plankton have resulted in long-term changes in cod recruitment in the North Sea (bottom-up control). On the other hand, both climate change and the improvement of trophic status in Geneva Lake favored the outbreak of whitefish during the years 90. Lower larval mortality and better recruitment are supposed to be linked to faster growth associated with warmer temperatures and better food conditions induced by better temporal overlap between larvae hatching and zooplankton development. (author)

  5. Goma de cajueiro (Anacardium occidentale: Avaliação das modificações químicas e físicas por extrusão termoplástica

    Directory of Open Access Journals (Sweden)

    Kelita C. S. Andrade

    2013-01-01

    Full Text Available Países tropicais, como o Brasil, são depositários de uma grande variedade de fontes de polissacarídeos vegetais conhecidos e outros ainda não explorados que possuem grande potencial em aplicações industriais. Alguns estudos reportam a possibilidade de aplicação industrial da goma exsudada do cajueiro em substituição à goma arábica devido à semelhança estrutural e química. Neste estudo propôs-se caracterizar comparativamente algumas propriedades destas duas gomas, antes e após dois tratamentos de extrusão termoplástica. Nos resultados de composição centesimal as amostras de goma de cajueiro in natura e processadas destacaram-se pelo alto teor de fibra solúvel. Por outro lado, apresentaram menor teor de minerais que a goma arábica. Por meio da análise de viscosidade rápida, foi observado que este parâmetro aumentou nas amostras de goma arábica processadas, enquanto nas amostras processadas de goma de cajueiro houve redução. Nos resultados da análise de difração de raios X predominou-se a conformação amorfa das cadeias poliméricas de ambas as amostras. A partir dos parâmetros avaliados, a goma de cajueiro poderia ser indicada como substituta da goma arábica.

  6. Influência do estiolamento e do ácido indolebutírico na formação de raízes do alporque de cajueiro Anão Precoce (Anacardium Occidentale L.

    Directory of Open Access Journals (Sweden)

    Francisco Célio Guedes Almeida

    1990-01-01

    Full Text Available O cajueiro é uma planta geralmente propagada por semente. Sua multiplicação vegetativa ainda não é comercialmente viável. Neste estudo os efeitos do estiolamento e do ácido indolebutírico (AIB na formação de raízes do alporque da planta de caju foram investigados. O estiolamento foi realizado 30 dias antes do início do processo de alporquia. Por ocasião do anelamento do ramo, o AIB foi aplicado. Após 30 dias da realização da alporquia, os ramos já enraizados foram removidos da planta progenitora. Em seguida foram colocados em saco plástico na casa de vegetação sob condições de irrigação intermitente. Os resultados sugeriram um prévio estiolamento do ramo antes do processo de alporquia e uma aplicação de AIB, por ocasião do anelamento para, garantir a sobrevivência do alporque após o desmame.The Cashew tree is usually propagated by seed since vegetative multiplication has not been fully satisfactory. In this work the effects of etiolation and AIB on air layered branches of the cashew tree were studied. Previous to airlayering procedure, the branches were etiolated during 30 days. The AIB was applied when the bark of the stem was cut. After 30 days the layers were removed from the parent plant at which roots stage were observed through the transparent bag. The rooted layers were planted in polyethylene bags and placed in a greenhouse under an intermitent-mist water spray condition. The results show that the previous etiolation and application of AIB to the exposed wound were beneficial to increase rooting and survival of cashew air layers.

  7. Un projet d’enquête : « la crise de 1300 » dans les pays de la Méditerranée occidentale 

    OpenAIRE

    Monique Bourin

    2009-01-01

    C’est un des dogmes de l’histoire médiévale de l’après-guerre qu’il y a un « automne du Moyen Âge » et qu’il commence dès la fin du xiiie siècle. Ce passage de la croissance aux malheurs des temps, qu’on datait autrefois du milieu du xive siècle, est désigné aujourd’hui sous le nom de « crise de 1300 ». Cette expression abrégée correspond en fait à une période de transition, large, presque séculaire, recouvrant en tout cas les années 1270-1330. Elle se distingue nettement de la stagnation qui...

  8. Les manuels utilisés dans l’enseignement du français en Russie au XVIIIe siècle: influences occidentales et leur réсeption en Russie

    Directory of Open Access Journals (Sweden)

    Serguey Vlassov

    2013-11-01

    Full Text Available «Учебники, используемые в преподавании французского языка в России в XVIII веке : западные влияния и их рецепция в России» Статья посвящена анализу грамматик, «школьных» и «домашних»  «разговоров», букварей, использовавшихся в России сначала в частном преподавании французского языка детям Петра Великого (цесаревичу Алексею и будущей императрице Елизавете Петровне, а также в частных пансионах, а затем в государственных учебных заведениях Санкт-Петербурга (в академической гимназии, в Сухопутном шляхетном кадетском корпусе и в Морском кадетском корпусе и Москвы (в гимназиях и, позднее, в Благородном пансионе при Московском университете. Как показало проведенное исследование, преподавание французского языка в России было сначала ориентировано на немецкие образцы, но затем французские модели учебников постепенно вытеснили своих немецких конкурентов благодаря адаптации к российским условиям прежде всего грамматики Ресто - в учебниках П. де Лаваля, Л. Бужо, Ж.-Р. Готье и других французских учителей, эмигрировавших в Россию при Елизавете Петровне и Екатерине II. Тем не менее влияние французской философской грамматики проявляется в преподавании французского языка в России лишь с 90-х годов XVIII в. – в грамматиках И. Соца (1790 и И. Фабиана (1799, так как ранее заимствования в грамматике Ресто из философской грамматики устранялись из переделок его грамматики в России. Кроме печатных источников, в статье рассматриваются некоторые неизданные материалы, хранящиеся в Архиве Академии наук, в Военно-морском архиве и в рукописных отделах Библиотеки Академии наук и Российской национальной библиотеки.

  9. Plutonium distribution and remobilization in sediments of the Rhone River mouth (North-Western Mediterranean); Distribution et remobilisation du plutonium dans les sediments du prodelta du Rhone (Mediterranee nord-occidentale)

    Energy Technology Data Exchange (ETDEWEB)

    Lansard, B

    2004-06-15

    The aim of the present study was to describe the distribution and remobilization of plutonium (Pu) in the sediments off the Rhone river mouth. Most of the {sup 238}Pu and {sup 239,240}Pu isotopes introduced into the Rhone River were discharged by the liquid effluents released from the Marcoule reprocessing plant, located 120 km upstream the river mouth. Due to its high affinity for particles and its long half life, {sup 238}Pu is a promising tracer to follow the dispersion of particulate matter from the Rhone River to the Mediterranean Sea. During the 3 REMORA cruises, sediment samples were specifically collected in the Rhone pro-delta area and more offshore on the whole continental shelf of the Gulf of Lions. The measurements of alpha emitters gave a first detailed spatial distribution of Pu isotope concentrations in surface sediments off the Rhone mouth. Using {sup 137}Cs concentrations and their correlations with Pu isotopes, we were able to give a first estimate of Pu inventories for the sediments of the study area. In 2001, plutonium inventories were estimated to 92 {+-} 7 GBq of {sup 238}Pu and 522 {+-} 44 GBq of {sup 239,240}Pu for an area of 500 km{sup 2} in front of the Rhone River mouth. Roughly, 50 % of these inventories are trapped in an area of 100 km{sup 2} corresponding to the extent of the Rhone pro-delta zone. In spring 2002, an ADCP, with current velocity and wave measurements, was moored off the Rhone River mouth. This unique in situ dataset highlights the major role of South-East swells in the erosion of pro-deltaic sediments and their dispersion to the South-Westward direction. Plutonium remobilization was examined using a new experimental design based on sediment resuspension processes studied within a linear recirculating flume. For Gulf of Lions sediments and for a given hydrodynamic stress, remobilization fluxes raised a maximum of 0.08 Bq.m{sup -2}.h{sup -1} for {sup 238}Pu and 0.64 Bq.m{sup -2}.h{sup -1} for {sup 239,240}Pu. A first plutonium budget determined for the study area indicates that at least 85 % of the Pu discharged over the 40 past years by the Marcoule reprocessing plant, are currently trapped in the sediments off the Rhone River mouth. Plutonium remobilization is potentially an important process but dispersion of contaminated sediments appeared to be limited in space. These results suggest a low exportation of particulate matter introduced by the Rhone River towards the continental shelf of the Gulf of Lions and the North-Western Mediterranean basin. (author)

  10. Découverte de coésite dans la ceinture métamorphique UHP d'âge Paléozoïque inférieur du Nord-Qaidam, Chine nord-occidentale

    Science.gov (United States)

    Yang, Jingsui; Xu, Zhiqin; Song, Shuguang; Zhang, Jianxin; Wu, Cailai; Shi, Rendeng; Li, Haibing; Brunel, Maurice

    2001-12-01

    Coesite and graphite were discovered as inclusions in zircon separates from pelitic gneiss associated with a large eclogite body in the North Qaidam UHP terrane. This finding suggests UHP metamorphism at pressures below the diamond stability field. This supports previous indirect UHP evidences, such as polycrystalline quartz inclusions in eclogitic garnet, quartz lamellae in omphacite and P- T estimates for both eclogite and garnet peridotite. The U/Pb and Sm/Nd isotopic ages from the North Qaidam eclogite indicated that continental subduction occurred in Early Palaeozoic, most probably in relation with the collision between the Sino-Korean and Yangtze plates.

  11. La guerra asimétrica. Una lectura crítica de la transformación de las doctrinas militares occidentales Asymmetric War. A Critical Reading of the Transformation of Western Military Doctrines

    Directory of Open Access Journals (Sweden)

    Raúl Zelik

    2011-12-01

    Full Text Available A pesar de que contradicen la concepción de ''conflictos de baja intensidad'', las ocupaciones de Irak y Afganistán están fuertemente influidas por los conceptos más antiguos de las ''guerras pequeñas'' -small wars- y de las ''intervenciones de bajo perfil'' -low footprint interventions-. El autor describe la evolución del concepto de 'guerra asimétrica' y se argumenta que la conducción asimétrica de la guerra ha sido un elemento de las estrategias bélicas de Occidente durante el siglo XX, mucho antes de que concluyera la confrontación de los bloques o aparecieran las llamadas ''nuevas guerras'' -new wars-. El autor identifica tres tendencias en la evolución de la guerra en conexión con los conflictos asimétricos: 1 una reorientación que conduce de los asuntos militares al problema del control social de la población; 2 una irregularización de la violencia, que implica una ruptura con las leyes y los acuerdos internacionales, y 3 la subcontratación -outsourcing- del ejercicio de la guerra a fuerzas irregulares aliadas, tales como corporaciones militares privadas, milicias, grupos paramilitares, señores de la guerra e incluso, crimen organizado.In spite of contradicting the concept of ''low intensity conflicts'', the military occupations of Iraq and Afghanistan are strongly influenced by older concepts of ''small wars'' and ''low footprint'' interventions. The author outlines the development of the notion of ''asymmetric war'' and argues that asymmetric warfare has been an element of Western military strategies throughout the 20th century-long before the end of the block confrontation and the emergence of so called ''new wars.'' The author identifies three tendencies of state warfare in the context of asymmetric conflicts: 1 a reorientation that leads from military issues to the problem of social control of the population, 2 an irregularization of state violence implying the breaking of laws and international conventions, and 3 the outsourcing of warfare to allied irregular forces such as private military companies, militias, paramilitary groups, warlords and even organized crime.

  12. Discovery, synthesis and SAR analysis of novel selective small molecule S1P4-R agonists based on a (2Z,5Z)-5-((pyrrol-3-yl)methylene)-3-alkyl-2-(alkylimino)thiazolidin-4-one chemotype.

    Science.gov (United States)

    Urbano, Mariangela; Guerrero, Miguel; Velaparthi, Subash; Crisp, Melissa; Chase, Peter; Hodder, Peter; Schaeffer, Marie-Therese; Brown, Steven; Rosen, Hugh; Roberts, Edward

    2011-11-15

    High affinity and selective S1P(4) receptor (S1P(4)-R) small molecule agonists may be important proof-of-principle tools used to clarify the receptor biological function and effects to assess the therapeutic potential of the S1P(4)-R in diverse disease areas including treatment of viral infections and thrombocytopenia. A high-throughput screening campaign of the Molecular Libraries-Small Molecule Repository was carried out by our laboratories and identified (2Z,5Z)-5-((1-(2-fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl)methylene)-3-methyl-2-(methylimino) thiazolidin-4-one as a promising S1P(4)-R agonist hit distinct from literature S1P(4)-R modulators. Rational chemical modifications of the hit allowed the identification of a promising lead molecule with low nanomolar S1P(4)-R agonist activity and exquisite selectivity over the other S1P(1-3,5)-Rs family members. The lead molecule herein disclosed constitutes a valuable pharmacological tool to explore the effects of the S1P(4)-R signaling cascade and elucidate the molecular basis of the receptor function.

  13. Molecular Detection of Mycotoxin Chemotypes of Seeds Borned Fusarium graminearum Clade on Maize%我国北方玉米子粒禾谷镰孢菌群产毒素化学型检测分析

    Institute of Scientific and Technical Information of China (English)

    董怀玉; 徐婧; 王丽娟; 刘可杰; 姜钰; 胡兰; 张明会; 徐秀德

    2014-01-01

    利用镰孢菌产毒素基因特异性引物,对分离自我国北方春玉米区玉米子粒的禾谷镰孢菌复合种群(Fusarium graminearum clade)的43株镰孢菌菌株进行产毒素化学型检测.结果表明,我国北方玉米子粒中携带的禾谷镰孢菌(包括F.graminearum和F.asiaticum)检测到2种产毒素化学型,F.graminearum只产生脱氧雪腐镰孢烯醇(Deoxynivalenol,DON),F.asiaticum可以产生脱氧雪腐镰孢烯醇(Deoxynivalenol,DON)和雪腐镰孢烯醇(Nivalenol,NIV).

  14. 七种百里香精油的主成分分析及其化学型%The Principle Component Analysis and Chemo-types of the Essential Oils from Seven Varieties of Thyme

    Institute of Scientific and Technical Information of China (English)

    黄健; 马莉; 姚雷; 吴亚妮

    2009-01-01

    百里香种类繁多,其精油的化学型是决定其应用领域的先决条件.本研究对7种百里香精油的20个主要化学成分进行主成分分析,将20个成分简化为3个主成分,3个主成分所提供的信息量占全部总信息量的91.91%,转换后的新变量用于进一步的聚类分析.聚类结果表明,在num15水平上可以将7种百里香分为3组,分别对应3个化学型,即百里香酚型、香芹酚型和香叶醇型,第一组包括德国冬百里香(Thymus vulgaris‘German')、法国细叶百里香(Thymus vulgaris‘French')、英国百里香(Thymus vulgaris‘English'),银斑百里香(Thymus vulgaris‘Silver Posie')和匍匐百里香(Thymus serpyllum),属于百里香酚化学型;宽叶百里香(Thymus pulegioides)和金边柠檬百里香(Thymus cariodorus‘Golden Queen')各自成组,分别属于香芹酚和香叶醇化学型.本研究探索了对百里香化学型进行划分的一种方法,为百里香精油的应用提供了更为准确的定位,同时也为目标品种的筛选和百里香种质资源创新提供理论依据.

  15. Identification and Assessment of Chemotype and Genotype of Cannabinoids in Cannabis sativa L.%大麻种质资源中大麻素化学型及基因型鉴定与评价

    Institute of Scientific and Technical Information of China (English)

    陈璇; 许艳萍; 张庆滢; 郭孟璧; 郭蓉; 杨明; 郭鸿彦

    2016-01-01

    大麻素(cannabinoids)是大麻植物中特有的次生代谢产物,主要成分为四氢大麻酚(THC,tetrahydrocannabinol)和大麻二酚(CBD,cannabidiol).本研究通过对我国不同来源地的23份大麻种质资源共69个单株材料中THC和CBD含量特征及其合成关键酶基因多态性进行分析,鉴定了我国大麻种质资源的大麻素化学型及基因型.结果显示,69个单株中大麻素含量差异显著,THC含量均值为0.56%,范围为0.01%~2.45%;CBD含量均值为0.53%,范围为0~2.24%;根据CBD/THC含量比值,大麻资源可划分为毒品型(占44.93%)、中间型(占20.29%)和纤维型(占34.78%)3种大麻素化学型,毒品型、中间型中分别有93.5%和71.4%的植株中THC含量>0.3%,纤维型植株中THC含量≤0.08%.3种化学型遗传位点(共显性位点B)的基因型分别为BT/BT、BT/BD和BD/BD;BT等位基因(THCAS)存在10个变异位点,氨基酸序列有4处变异,BD等位基因(CBDAS)存在4个变异位点,均为同义突变.根据THCAS和CBDAS基因多态性,设计了一个共显性复合PCR分子标记,可准确鉴定出大麻3种化学型.研究结果揭示了我国大麻种质资源中大麻素含量、化学型和基因型三者之间的关系,可为大麻素遗传研究与利用提供理论依据.

  16. Chemotype and Genotype of Cannabinoids in Hemp Landrace from Southern Yunnan%滇南农家大麻品种中大麻素化学型及基因型研究

    Institute of Scientific and Technical Information of China (English)

    陈璇; 郭孟璧; 张庆滢; 许艳萍; 郭鸿彦; 杨明; 杨清辉

    2013-01-01

    以1个滇南农家大麻品种群体为研究对象,通过化学分析及同源克隆方法,研究了21个单株中2种主要大麻素——四氢大麻酚(THC)和大麻二酚(CBD)的化学型和基因型,以揭示大麻素含量、化学型以及基因型三者之间的关系,为工业大麻新品种选育提供理论依据.研究表明:(1)化学检测结果显示,21个单株均含有THC,THC含量在0.07%~1.35%之间,其中7个单株仅含THC,5个单株含THC和微量CBD,9个单株同时含有THC和CBD,CBD含量范围为0~0.58%.(2)CBD/THC比值显示,该群体仅存在毒品型和中间型2种化学型,且中间型大麻中THC和CBD含量显著正相关.(3)基因扩增及测序分析结果显示,该群体为基因型杂合群体,群体内THCA合成酶基因存在5个变异位点,CBDA合成酶基因存在2个变异位点,但变异位点和THC及CBD的含量无直接关系.(4)群体内单株的基因型和化学型完全对应,且THCA合成酶基因及CBDA合成酶基因可作为分子标记来鉴定单株化学型.

  17. Effect of carbon monoxide on respiration in higher plants. [Fagopyrum esculentum L. ; Delphinium Ajacis L. ; Raphanus sativus L. ; Pyrus Malus L. ; Beta vulgaris L. ; Phaseolus vulgaris L. ; Phaseolus limensis L. ; Medicago pratense L. ; Nicotiana Tabacum L. ; Cucumis sativus L. ; Helianthus annuus L

    Energy Technology Data Exchange (ETDEWEB)

    Webster, G.C.

    1954-01-01

    The effect of carbon monoxide and light on the respiration of a number of plant tissues were examined. The respiration of root or other tissue was measured at 25/sup 0/C by standard manometric techniques in a ratio of 95% CO and 5% O/sub 2/. The respiration of all eleven tissues studied was strongly inhibited by carbon monoxide. In ten of the eleven cases examined the inhibition was largely or completely eliminated by irradiation of the tissue with light. The evidence fairly well precludes the participation of a tyrosinase and definitely supports the participation of a cytochrome oxidase in respiration. 5 references, 1 table.

  18. Herinplantziekte en groeiproblemen in zomerbloemen

    NARCIS (Netherlands)

    Slootweg, G.; Breeuwsma, S.J.; Helm, van der F.P.M.

    2010-01-01

    In een bijeenkomst met kwekers en voorlichters zijn herinplantproblemen in de praktijk geïnventariseerd. Aangegeven werd dat vooral behoefte is aan inzicht in herinplantproblemen bij pioenrozen en groeiproblemen bij Campanula en Delphinium. Onderzoek heeft aangetoond dat aaltjes niet de oorzaak zijn

  19. Herinplantziekte en groeiproblemen in zomerbloemen

    OpenAIRE

    Slootweg, G.; Breeuwsma, S.J.; Helm, van der, F.P.M.

    2010-01-01

    In een bijeenkomst met kwekers en voorlichters zijn herinplantproblemen in de praktijk geïnventariseerd. Aangegeven werd dat vooral behoefte is aan inzicht in herinplantproblemen bij pioenrozen en groeiproblemen bij Campanula en Delphinium. Onderzoek heeft aangetoond dat aaltjes niet de oorzaak zijn. Zowel kwekers als voorlichters vermoeden een rol voor toxische stoffen uit de planten zelf bij het ontstaan van deze problemen (allelopathie).

  20. Coosa River Storage Annex, Talladega, Alabama. Environmental Investigation Report. Volume 2 of 2

    Science.gov (United States)

    1992-09-01

    jointtail Crataegus harbisoni hawthorn Croomnia pauciflora croomia Croton alabamensis Alabama croton Cuscuta Izarperi lovevine, dodder Cypripediunz...kentuckiense Kentucky lady’s-slipper Delphinium exalt atumnz tall larkspur Echinacca laevigata purple coneflower Helianthus eggertii glade sunflower Hexastylis...flava southern rein orchid Platanth era integra yellow fringeless orchid Platanth era peramoena purple fringeless orchid Polyninnia laevigata Tennessee

  1. Environmental Compliance Assessment Protocol - Centers for Disease Control and Prevention (ECAP-CDC), West Virginia Supplement

    Science.gov (United States)

    1994-11-01

    Water loosestrife Delphinium exaltatum Tall larkspur Descurainia pinnata Tansy-mustard Desmodium lineatum A tick-trefoil (continued) 5 -10 Appendix 5-1...continued) Scientific Name Common Name Desmodium pauciflorum A tick-trefoil Digilariafiliformis Slender crabgrass Disporum maculatum Mandarin

  2. Chemical and morphological diversity in wild populations of Mentha longifolia in Israel.

    Science.gov (United States)

    Segev, Daniel; Nitzan, Nadav; Chaimovitsh, David; Eshel, Amram; Dudai, Nativ

    2012-03-01

    Populations of Mentha longifolia, an endangered species in Israel, were tested for essential oil composition and conservational ability. In 2002-2003, 25 wild populations country-wide were tested, indicating population divergence into two chemotypes. Chemotype A was characterized by high levels of menthone and pulegone, and chemotype B by high levels of piperitenone oxide and piperitone oxide. Chemotype A was more abundant (22 of 25 populations) than chemotype B (11 of 25 populations). However, a chemotype/population interaction was not recorded (P > 0.05). In spring 2003, seven of the 25 wild populations were resampled, propagated, and cultivated at the Newe Ya'ar campus. Then, in 2004, the propagated plants were tested for essential oil composition. The propagated plants maintained the essential oil composition as well as the chemotype-frequency distribution of the original wild population from which they were obtained. Since a chemotype/population interaction was not recorded, and the cultivated plants displayed the wild population essential oil composition, it can be concluded that i) the chemotype diversity is genetically based, and ii) the M. longifolia populations sampled can be horticulturally conserved.

  3. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations.

    Science.gov (United States)

    Satyal, Prabodh; Murray, Brittney L; McFeeters, Robert L; Setzer, William N

    2016-10-27

    Thyme (Thymus vulgaris L.) is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1) linalool; (2) borneol; (3) geraniol; (4) sabinene hydrate; (5) thymol; (6) carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography-mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%); the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%); the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%); and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%). A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris.

  4. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations

    Directory of Open Access Journals (Sweden)

    Prabodh Satyal

    2016-10-01

    Full Text Available Thyme (Thymus vulgaris L. is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1 linalool; (2 borneol; (3 geraniol; (4 sabinene hydrate; (5 thymol; (6 carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography–mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%; the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%; the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%; and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%. A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris.

  5. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations

    Science.gov (United States)

    Satyal, Prabodh; Murray, Brittney L.; McFeeters, Robert L.; Setzer, William N.

    2016-01-01

    Thyme (Thymus vulgaris L.) is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1) linalool; (2) borneol; (3) geraniol; (4) sabinene hydrate; (5) thymol; (6) carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography–mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%); the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%); the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%); and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%). A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris. PMID:28231164

  6. Estimative of relative stiffness of the exudate gum polysaccharides; Estimativa do parametro de rigidez (B)de gomas de exudatos regionais

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Marilia A.; Paula, Regina C.M. [Ceara Univ., Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica]. E-mail: rpaula@dqoi.ufc.br

    2001-07-01

    The Smidsrod empirical stiffness parameter (B) of A. occidentale and A. lebbeck gum were determined using the correlation of intrinsic viscosity [{eta}] with ionic strength. The B value of 0.204 and 0.193 found respectively for A. occidentale and A. lebbeck suggests a flexible the molecule. The ionic strength has a greater influence on the [{eta}]. The decrease of [{eta}] increase of I, from 0.01 M to 0.1 M of NaCl, is higher for A. lebbeck (89%) than for A. occidentale (19%). (author)

  7. The volcanism of the western part of the Los Frailes Meseta (Bolivia): a representative example of the Andean volcanism since the Upper Oligocene; Le volcanisme de la bordure occidentale de la Meseta de Los Frailes (Bolivie): un jalon representatif du volcanisme andin depuis l`Oligocene superieur

    Energy Technology Data Exchange (ETDEWEB)

    Leroy, L. [Nancy-1 Univ., 54 (France); Jimenez, N.

    1996-12-31

    The Los Frailes Meseta (Bolivia) is one of the large tertiary ignimbritic fields of the inner volcanic arc from Central Andes (Central Volcanic Zone. CVZ), in contact zone between the Altiplano to the west and the Eastern Cordillera to the east. Field observations and mineralogical and geochemical studies (major and trace elements) lead to distinguish two types of volcanism in the western border to the Meseta. During the Middle Miocene and Pliocene, the volcanic activity can be subdivided into three pyroclastic emission cycles, the Larco, Coroma and Pliocene ignimbrites, the first two being separated by the Quechua 2 orogeny. All these ignimbrites are very similar and correspond to peraluminous rhyolites to rhyodacites. In the studies area, the Coroma cycle is the only one where an ignimbrite-less evolved resurgent dome association can be observed. Beside these ignimbrites, isolated small lava flows and domes overlay and/or intrude all the other formations. They are meta-aluminous lavas with a shoshonitic affinity. A quaternary age can be attributed to his second volcanism. These two volcanic types are well-known in the CVZ and are related to the different deformation stages, either compressional or extensional, which occur alternately in the Cordillera since 26 Ma. (authors). 61 refs., 12 figs., 3 tabs.

  8. Cambios de gravedad de origen tectónico en la transición entre las Sierras Pampeanas Occidentales y la Precordillera Sanjuanina Gravity changes of tectonic origin in the transition between western Sierras Pampeanas and Precordillera of San Juan.

    Directory of Open Access Journals (Sweden)

    Francisco Ruiz

    2011-12-01

    Full Text Available El fenómeno sísmico está estrechamente ligado a cambios de gravedad producto de la deformación y de cambios de densidad en la corteza. Se ha establecido una red de control geodésico con el propósito de establecer relaciones espacio-temporales en la evolución del campo gravitatorio producido por actividad tectónica. La red está compuesta por 65 puntos fijos bien materializados y cubre un área de 3.500 km2 con la ciudad de San Juan en su centro. Diez años de mediciones periódicas han permitido evaluar las velocidades de variación de la intensidad del campo gravitatorio; éstas se ajustan razonablemente por el modelo estadístico de regresión lineal de las observaciones de la gravedad versus tiempo. Los resultados señalan el levantamiento actual de la Precordillera Oriental, que se refleja en aumentos de gravedad (g en las estaciones situadas al oeste del fallamiento Villicum-Ullum-Zonda y en la disminución de g al este de la estructura. identificamos tres segmentos activos: a la falla de La Laja con fuerte disminución de g, b el segmento Maradona-Cerro Bayo, con aumentos de g al oeste de la sierra Chica de Zonda y c una disminución de g en la falla de La Rinconada. Los datos muestran actividad tectónica en el sistema de fallas del Tulum que exhibe un gradiente en la dirección del lineamiento Cerro Valdivia-Cerro Barboza-Sierra de Pie de Palo que se incrementa hacia el este. Sus variaciones máximas negativas se observan al este del cerro Barboza. Mapas de anomalías magnéticas y gravimétricas señalan patrones en el basamento coincidentes con estas estructuras activas.Earthquake is closely tied up with gravity changes on the basis of crustal deformation and crustal density changes. A network of areal geodetic control was set up with the purpose of establishing space-time relations in the evolution of the gravity field produced by tectonic activity. The network is composed by 65 bench marks well materialized in the field, and extends over an area of about 3,500 km2 centered in the San Juan city. Ten years of gravity periodic measurements have allowed to evaluate the speeds of the gravitational intensity field variations. These gravity changes adjust reasonably well with the statistical lineal regression model applied to the gravity versus time observations. The observed data support the current uplift of the Eastern Precordillera, reflected in increases of gravity (g in all bench marks located westwards of the Villicum-Ullum-Zonda fault and in the decrease of gravity eastwards of this structure. We identify three segments of larger activity: a La Laja fault with strong decreases of g, b Maradona-Cerro Bayo segment, with increases of g westwards of the Sierra Chica de Zonda and c decreases of g at La Rinconada fault. The data show tectonic activity in the Tulum faults system, gravity changes have lined up in the Cerro Valdivia-Cerro Barboza-Sierra de Pie de Palo lineament and increase to the east. The largest variations are observed eastwards of the Barboza hill with a minimum of g. Magnetic and gravity anomaly maps indicate basement patterns consistent with these active structures.

  9. Contribution à l'étude des substances carbonées dans les bassins paleozoiques de la bordure occidentale du massif de l'air (République du Niger) Implications sédimentologiques et géochimiques

    OpenAIRE

    Harouna, Moussa

    1991-01-01

    LES SEDIMENTS DU CARBONIFERE INFERIEUR DE LA REGION D'AGADES, TYPIQUEMENT MARINS AU NORD (ARGILITES DU TALAK), PASSENT LATERALEMENT A DES FACIES LAGUNO-LACUSTRES DANS LA PARTIE MERIDIONALE. CES FACIES RECELENT DES NIVEAUX DE CHARBON BIEN DEVELOPPES A ANOU-ARARENE ET A SEKIRET. LES SEQUENCES FLUVIO-DELTAIQUES DU GUEZOUMAN ET DU TARAT, ATTRIBUEES AU NAMURIEN-WESTPHALIEN CONTIENNENT DE LA MATIERE ORGANIQUE DISPERSEE, RESPONSABLE DE LA CONCENTRATION DE L'URANIUM. LE LINEAMENT MERIDIEN DE IN AZAWA...

  10. Application de la télédetection satellitaire à l’évaluation des données pour l’aménagement du territoire en Afrique occidentale intertropicale : exemple des provinces du Gourma et de la Sissili, sud du Burkina Faso

    OpenAIRE

    Drabo, Toro

    2006-01-01

    L’Afrique, de par la diversité de ses écosystèmes, de la richesse de ses ressources et de par la confrontation de deux conceptions d’aménagement, l’une africaine l’autre externe, offre à la télédétection satellitaire l’un des meilleurs champs d’application. Cependant, une conscience claire des besoins spécifiques en informations par rapport aux objectifs à atteindre manque. Ceci est dû à l’insuffisance d’une politique de développement cohérent, dont la dimension spatiale n’est rien d’autre qu...

  11. Pierre Swiggers, Histoire de la pensée linguistique. Analyse du langage et réflexion linguistique dans la culture occidentale, de l'Antiquité au XIXème siècle, Presses universitaires de France, Paris 1997

    Directory of Open Access Journals (Sweden)

    Mitja Skubic

    1997-12-01

    Full Text Available Pierre Swiggers, professeur de linguistique theéorique à l' Universite de Louvain, peut être considéré un des esprits des plus pénétrants de l'epistémologie du langage humain. Notre revue a déjà eu l'honneur de le compter parmis ses collaborateurs: avant de publier sa récente étude sur les théories linguistiques du XXème siècle, ii y avait publié un article de réflexion méthodologique, Comparaison des langues et grammaire comparée (vol. 28 et une analyse des Frammenti grammaticali latino-friulani, exercices de grammaire qui a la moitie du XIVeme siècle servaient aux futurs notaires de I'école de Cividale, dans le Frioul, à apprendre le latin. Cet article a également un titre très significatif, Su alcuni principi della grammatologia latino-volgare; significatif dans le sens où I' auteur semble avoir une inclination particulière pour les langues vernaculaires, pour il volgare, en reprenant le terme de Dante. Il suffit de consulter la riche bibliographie (pp. 265-307 pour s'en rendre compte; l' auteur y apparaît avec plus de 40 occurences, toutes strictement liées aux sujets dont traite le livre dont nous allons parler.

  12. Mise en évidence de déformations en faille inverse avec ruptures de surface cosismiques dans des dépôts colluviaux würmiens du versant nord du mont Ventoux (Provence occidentale, France)Evidence of reverse faulting and coseismic surface ruptures in Würm colluvial deposits from the Mt Ventoux northern slope (Western Provence, France)

    Science.gov (United States)

    Dutour, Alain; Philip, Hervé; Jaurand, Erwan; Combes, Philippe

    In western Provence (France), brittle deformation of Quaternary age occurring in the vicinity of the Nı̂mes and Durance faults has been linked to palaeoseisms of significant magnitude. Our new observations made on the southern rim of the Tertiary Malaucène Basin, in the continuation of a thrust to the north of Mt Ventoux, present evidence for reverse faulting deformation in deposits of a Würm colluvial fan. The analysis of a trench section provides clear evidence for: (1) the development of two successive surface ruptures and degradation of associated scarps during the Mid-Upper Würm, and, (2) the continuation of the reverse fault within the Oligocene basement. These tectonic events were associated with earthquakes of at least 6 in magnitude. To cite this article: A. Dutour et al., C. R. Geoscience 334 (2002) 849-856.

  13. In pursuit of natural product leads: synthesis and biological evaluation of 2-[3-hydroxy-2-[(3-hydroxypyridine-2-carbonyl)amino]phenyl]benzoxazole-4-carboxylic acid (A-33853) and its analogues: discovery of N-(2-benzoxazol-2-ylphenyl)benzamides as novel antileishmanial chemotypes.

    Science.gov (United States)

    Tipparaju, Suresh K; Joyasawal, Sipak; Pieroni, Marco; Kaiser, Marcel; Brun, Reto; Kozikowski, Alan P

    2008-12-11

    The first synthesis and biological evaluation of antibiotic 31 (A-33853) and its analogues are reported. Initial screening for inhibition of L. donovani, T. b. rhodesiense, T. cruzi, and P. falciparum cultures followed by determination of IC(50) in L. donovani and cytotoxicity on L6 cells revealed 31 to be 3-fold more active than miltefosine, a known antileishmanial drug. Compounds 14, 15, and 25 selectively inhibited L. donovani at nanomolar concentrations and showed much lower cytotoxicity.

  14. Adubação orgânica na produção, rendimento e composição do óleo essencial da alfavaca quimiotipo eugenol Organic fertilization in the production, yield and chemical composition of basil chemotype eugenol

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Biasi

    2009-03-01

    Full Text Available Este trabalho foi realizado na UFPR, município de Pinhais-PR, de outubro de 2005 a junho de 2006. Avaliou-se a resposta do Ocimum gratissimum à adubação orgânica com composto de esterco de carneiro, verificando a viabilidade dessa prática para o aumento no rendimento de biomassa, na quantidade e qualidade de seu óleo essencial. As mudas foram plantadas com espaçamento de 0,5 m entre plantas dentro das parcelas e 1 m entre as parcelas. Testaram-se 3 doses de adubação com composto orgânico (4; 8 e 12 kg m-2 e uma testemunha, com 5 repetições e 8 plantas por parcela. As colheitas foram realizadas aos 150 e 226 dias após o plantio, sendo avaliado o rendimento de massa fresca e massa seca total e de folhas, flores e caule, o teor de óleo essencial das folhas e flores e a sua composição por cromatógrafo a gás acoplado a um detector de massas (CG-MS. Houve diferença entre os tratamentos, quanto ao rendimento de biomassa na primeira colheita, onde o tratamento com 8 kg m-2 de composto orgânico foi superior à testemunha sem adubação, para a massa fresca de flores por planta e massa seca total e de flores por planta, mas não diferiu dos outros níveis de adubação. Na segunda colheita não houve diferença entre os tratamentos. Quanto ao rendimento de óleo essencial não houve diferença entre os tratamentos nas duas colheitas. Também não foram observadas grandes variações nos componentes do óleo essencial decorrente dos tratamentos aplicados. Entretanto, ocorreram variações na composição do óleo essencial quando extraído das folhas e das flores, sendo o teor médio de eugenol de 90,4% nas folhas e 80,8% nas flores, na colheita em março. Já na colheita em junho, houve uma redução do teor de eugenol nas folhas e uma elevação dos teores, principalmente de alfa trans trans farneseno, beta bisaboleno, beta cariofileno, germacrene D e alfa selineno.This work was carried out at the Universidade Federal do Parana, Paraná State, Brazil, from October 2005 to June 2006. The effect of organic fertilization with sheep manure was evaluated on Ocimum gratissimum development and the viability of this practice was determined to increase biomass and essential oil yield and quality. The plants were spaced 0.5 m from each other in the experimental units which were separated in 1 m. Three rates of organic fertlization (4; 8 and 12 kg m-2 were compared to control (without fertilizers, with 5 replications and 8 plants by experimental unit. Plants were harvested 150 and 226 days after planting, and the total, leaves, flowers and stems fresh and dried biomass, essential oil yield from leaves and flowers and composition by GC/MS beign evaluated. There was significant difference among the treatments on biomass production at the first harvest, where the plants treated with 8 kg m-2 of organic fertilizer presented higher flowers fresh biomass, and flowers and total dry biomass than plants without fertilizer (control plants, but with no difference compared to other organic fertilization levels. At the second harvest there were no differences among treatments. Regarding the essential oil yield no differences were found comparing the organic fertilization levels in both harvest times. In addition, the essential oil composition was similar on plants from all treatments. However, some variations in composition were observed when the essential oil was extracted from flowers and leaves, where the eugenol percentage was 90.4% on leaves and 80.8% on flowers at the first harvest. At the second harvest, a reduction of eugenol content and an increase of alpha trans trans farnesene, beta bisabolene, beta cariophilene, germacrene D and alfa selineno was found.

  15. デルフィニウムの花(新鮮がく片)のアントシアニン色素と花色の数理解析法

    OpenAIRE

    橋本, 文雄; 福田, 祥子; 前田, 寛子; 田中, 見佳; 清水, 圭一; 樗木, 直也; 坂田, 祐介; 石黒, 悦爾; Hashimoto, Fumio; Fukuda, Shoko; Maeda, Hiroko; Tanaka, Mika; Shimizu, Keiichi; Chishaki, Naoyuki; SAKATA, Yusuke

    2010-01-01

    The relationship between sepal hue and major sepal anthocyanins of cyanic Delphinium flowers, i.e., violdelphin (2) and cyanodelphin (3), is discussed. The mode of change in flower color in vivo was derived as a numerical equation relating two kinds of sepal pigments, from violdelphin [VD] (2) to cyanodelphin [CD] (3) for bluish flowers or from tulipanin [TP] (1) to [VD] (2) for purplish flowers, during anthesis, for changing flower hue from purple to blue or from pink to purple, respectiv...

  16. Development, anatomy, and genetic control of some teratological phenotypes of Ranunculaceae flowers

    Directory of Open Access Journals (Sweden)

    Florian Jabbour

    2016-04-01

    Full Text Available Teratological organisms originate from developmental anomalies, and exhibit structures and a body organization that deviate from the species standard. These monsters give essential clues about the formation and evolutionary significance of the wild-type groundplan. We focus on flower terata, which can be affected in their sterile and/or fertile organs, with special emphasis on the Ranunculaceae. The diversity of perianth shapes and organizations in flowers of this family is huge, and is even increased when anomalies occur during organo- and/or morphogenesis. To begin with, we synthesize the observations and research conducted on the Ranunculacean floral terata, following the most recent phylogenetic framework published in 2016 by our team. Then, we report results regarding the morphology of developing meristems, the anatomy of buds, and the genetic control of selected teratological phenotypes of Ranunculaceae flowers. We focus on species and horticultural varieties belonging to the genera Aquilegia, Delphinium, and Nigella. Wildtype flowers of these species are actinomorphic (Aquilegia, Nigella or zygomorphic (Delphinium, spurred (Aquilegia, Delphinium or with pocket-like petals (Nigella. Last, we discuss the evolutionary potential of such teratological phenotypes when they occur in the wild.

  17. Identification of antimicrobial properties of cashew, Anacardium ...

    African Journals Online (AJOL)

    Michael Horsfall

    ABSTRACT: The antimicrobial capabilities of plant extract derived from the leaves of the cashew plant, ... Medicinal plants are of great importance to the health ... Harcourt, Rivers State. .... occidentale should involve refining or purifying and.

  18. Hypoglycemic Effect of Methanolic Extract of Anacardium ...

    African Journals Online (AJOL)

    Chigo Okwuosa

    Page 1 ... occidentale in diabetic rats and its prophylactic activity against the diabetogenic action of streptozotocin This study evaluated the ... a low sugar and carbohydrate diet accompanied with ..... Genetically Type 2 Diabetic Mice. Biol.

  19. Short Communication

    African Journals Online (AJOL)

    mutans and Staphylococcus aureus recovered from dental caries ... (recovered previously from pus samples of burn patients) were ... were inoculated into sterile Nutrient broth tubes (in .... Fermentation of cashew (Anacardium occidentale L.).

  20. Los senderos de la idolatria: el viaje de Vazquez de Espinosa por los Altos de Arica, 1618

    National Research Council Canada - National Science Library

    Marsilli, Maria N; Cisternas, Priscilla

    2010-01-01

    El articulo examina el "Compendio y Descripcion de las Indias Occidentales" elaborado por el carmelita Antonio Vazquez de Espinosa en los segmentos del texto dedicados a su visita a los Altos de Arica...

  1. Determination of Heavy Metals in Leaves of Mangifera Indica ...

    African Journals Online (AJOL)

    USER PC

    Anacardium Occidentale L. in Trikania Industrial Area, Kaduna-Nigeria. ... metals from soil, water or air and retain them ... Administration (OSHA), European Union .... Heavy Metals from Wastewater using ... Biotechnology 5 (12): 1167-1176. 2.

  2. Il luogo nella formazione. L'incidenza simbolica degli elementi pre-formali nelle pratiche educative

    OpenAIRE

    Botto,

    2013-01-01

    1.Lo sfondo teoretico Il canone filosofico occidentale. Sulla base di un confronto con le più significative posizioni acquisite dal dibattito ontologico svoltosi nello scorso secolo – in particolare seguendo la traiettoria tracciata dal pensiero di M. Heidegger, J. Derrida e J.-L. Nancy – nella prima parte dello studio si approda a una preliminare fissazione di quello che, nella tradizione filosofica occidentale, si è imposto come un vero e proprio canone. Una colonna vertebrale rispetto...

  3. [Effect of several extracts derived from plants on the survival of larvae of Aedes fluviatilis (Lutz) (Diptera: Culicidae) in the laboratory].

    Science.gov (United States)

    Consoli, R A; Mendes, N M; Pereira, J P; Santos, B de S; Lamounier, M A

    1988-01-01

    The larvicidal properties of 34 plant extracts were tested against Aedes fluviatilis (Lutz) (Diptera: Culicidae) larvae, at 100, 10 and 1 ppm concentrations; 26.6% of the extracts enhanced larval mortality (alpha = 0.05) at 100 ppm (Anacardium occidentale, Agave americana, Allium sativum, Coriandrum sativum, Nerium oleander, Spatodea campanulata, Tibouchina scrobiculata and Vernonia salzmanni). Anacardic acid (A. occidentale) was effective at 10 ppm and A. sativum (crude extract) at 1 ppm.

  4. Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene

    NARCIS (Netherlands)

    Bertea, C.M.; Schalk, M.; Karp, F.; Maffei, M.; Croteau, R.

    2001-01-01

    ( )-Menthofuran is an undesirable monoterpenoid component of peppermint (Mentha x piperita) essential oil that is derived from the ,-unsaturated ketone ( )-pulegone. Microsomal preparations, from the oil gland secretory cells of a high ( )-menthofuran-producing chemotype of Mentha pulegium,

  5. Intra-population chemical polymorphism in Thymus pannonicus All. growing in Slovakia.

    Science.gov (United States)

    Maggi, Filippo; Caprioli, Giovanni; Papa, Fabrizio; Sagratini, Gianni; Vittori, Sauro; Kolarčik, Vladislav; Mártonfi, Pavol

    2014-01-01

    Solid-phase microextraction technique coupled with gas chromatography-flame ionisation detector and gas chromatography-mass spectrometry was used to investigate the inter- and intra-population chemical polymorphism in volatile constituents of three populations of Thymus pannonicus growing in Slovakia. To have an idea on what really the plant emits under different environmental and physiological conditions, a total of 86 individuals were separately analysed using low extraction temperatures (30°C). Data were analysed using a chemometric approach such as the principal component analysis which revealed a significant intra-population variability with the existence of up to four chemotypes: the most abundant is the p-cymene/γ-terpinene chemotype, followed by the geraniol and linalool chemotypes, and, only in one population, by the γ-muurolene/(E)-caryophyllene chemotype. This differentiation of individual plants inside the populations allows better adaptation to ecological conditions of localities and is probably connected with intrinsic genetic diversity of the species.

  6. Global population structure and adaptive evolution of aflatoxin producing fungi

    Science.gov (United States)

    We employed interspecific principal component analyses for six different categories (geography, species, precipitation, temperature, aflatoxin chemotype profile, and mating type) and inferred maximum likelihood phylogenies for six combined loci, including two aflatoxin cluster regions (aflM/alfN and...

  7. Melanin concentrating hormone receptor 1 (MCHR1) antagonists - Still a viable approach for obesity treatment?

    DEFF Research Database (Denmark)

    Högberg, T.; Frimurer, T.M.; Sasmal, P.K.

    2012-01-01

    Obesity is a global epidemic associated with multiple severe diseases. Several pharmacotherapies have been investigated including the melanin concentrating hormone (MCH) and its receptor 1. The development of MCHR1 antagonists are described with a specific perspective on different chemotypes...

  8. Reference: 594 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available nction mutant hig1-1 contained low levels of glucosinolates. Overexpression of the related transcription fac...the mutant chemotype along with a severe high-auxin growth phenotype. Overexpression of MYB122, another clos

  9. The Chemical Diversity of Lantana camara: Analyses of Essential Oil Samples from Cuba, Nepal, and Yemen.

    Science.gov (United States)

    Satyal, Prabodh; Crouch, Rebecca A; Monzote, Lianet; Cos, Paul; Awadh Ali, Nasser A; Alhaj, Mehdi A; Setzer, William N

    2016-03-01

    The aerial parts of Lantana camara L. were collected from three different geographical locations: Artemisa (Cuba), Biratnagar (Nepal), and Sana'a (Yemen). The essential oils were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry. A cluster analysis of 39 L. camara essential oil compositions revealed eight major chemotypes: β-caryophyllene, germacrene D, ar-curcumene/zingiberene, γ-curcumen-15-al/epi-β-bisabolol, (E)-nerolidol, davanone, eugenol/alloaromadendrene, and carvone. The sample from Cuba falls into the group dominated by (E)-nerolidol, the sample from Nepal is a davanone chemotype, and the sample from Yemen belongs to the β-caryophyllene chemotype. The chemical composition of L. camara oil plays a role in the biological activity; the β-caryophyllene and (E)-nerolidol chemotypes showed antimicrobial and cytotoxic activities.

  10. Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene

    NARCIS (Netherlands)

    Bertea, C.M.; Schalk, M.; Karp, F.; Maffei, M.; Croteau, R.

    2001-01-01

    ( )-Menthofuran is an undesirable monoterpenoid component of peppermint (Mentha x piperita) essential oil that is derived from the ,-unsaturated ketone ( )-pulegone. Microsomal preparations, from the oil gland secretory cells of a high ( )-menthofuran-producing chemotype of Mentha pulegium, transfor

  11. Bergamot versus beetle: evidence for intraspecific chemical specialization.

    Science.gov (United States)

    Keefover-Ring, Ken

    2015-11-16

    A large proportion of phytophagous insects show host plant specificity (monophagy or oligophagy), often determined by host secondary chemistry. Yet, even specialists can be negatively affected by host chemistry at high levels or with novel compounds, which may manifest itself if their host species is chemically variable. This study tested for reciprocal effects of a specialist tortoise beetle (Physonota unipunctata) feeding on a host plant (Monarda fistulosa) with two monoterpene chemotypes [thymol (T) and carvacrol (C)] using a controlled field experiment where larvae fed on caged plants of both chemotypes, haphazardly collected natural plants with and without beetle damage, and growth chamber experiments where larvae that hatched and briefly fed on one chemotype were reared on either chemotype. In the field experiment, plant chemotype did not affect larval weight or length, but did influence larval survival with almost 8.3 % more surviving on T plants. Herbivores reduced seed head area (86.5 % decrease), stem mass (41.2 %) and stem height (21.1 %) of caged plants, but this was independent of host chemotype. Natural plants experienced similar reductions in these variables (74.0, 41.4 and 8.7 %) and T chemotypes were more frequently damaged. In the growth chamber, larval relative growth rate (RGR) differed for both feeding history and year. Larvae from T natal plants reared on T hosts grew at almost twice the rate of those from C and reared on T. Larvae from either T or C natal plants reared on C plants showed intermediate growth rates. Additional analyses revealed natal plant chemotype as the most important factor, with the RGR of larvae from T natal plants almost one-third higher than that of those from C natal plants. These cumulative results demonstrate intraspecific variation in plant resistance that may lead to herbivore specialization on distinct host chemistry, which has implications for the evolutionary trajectory of both the insect and plant species.

  12. Essential oils from Leptospermums of the Sunshine Coast and Northern Rivers Regions

    Directory of Open Access Journals (Sweden)

    Windsor Sarah Alison

    2012-05-01

    Full Text Available Abstract Background Around the turn of this century, the oil yield and chemical composition of Australian Leptospermum species was analysed. Since that time, research has been focused on their use as phytomedicines. The oil yield and composition of essential oils from Australian Leptospermum species directly impacts their commercialisation for medicinal use. Results The essential oils from Leptospermum (L. juniperinum, L. laevigatum, L. liversidgei, L. polygalifolium, L. semibaccatum, L. speciosum, L. trinervium and L. whitei have been examined from specimens in the Sunshine Coast (Queensland and Northern Rivers (New South Wales Regions. Both chemotypes of L. liversidgei were observed. However, only chemotype II of L. semibaccatum and chemotype I of L. trinervium were identified. The only subspecies observed of L. polygalifolium was L. polygalifolium wallum. Conclusions L. liversidgei chemotypes I and II have the potential for phytomedical use as antibacterial or anti-inflammatory agents. Chemotype I has the potential for use as an insect repellent and chemotype II may provide antifungal activity.

  13. Effect of different liming levels on the biomass production and essential oil extraction yield of Cunila galioides Benth.

    Science.gov (United States)

    Mossi, A J; Pauletti, G F; Rota, L; Echeverrigaray, S; Barros, I B I; Oliveira, J V; Paroul, N; Cansian, R L

    2012-11-01

    Poejo is an aromatic and medicinal plant native to highland areas of south Brazil, in acid soils with high Al3+ concentration. The main objective of the present work was to evaluate the effect of liming on the extraction yield of essential oil of three chemotypes of poejo (Cunila galioides Benth). For this purpose, the experiments were performed in a greenhouse, using 8-litre pots. The treatments were four dosages of limestone (0, 3.15, 12.5, and 25 g.L(-1)) and a completely random experimental design was used, with four replications and three chemotypes, set up in a 3 × 4 factorial arrangement. The parameters evaluated were dry weight of aerial parts, essential oil content and chemical composition of essential oil. Results showed that liming affects the biomass production, essential oil yield and chemical composition, with cross interaction verified between chemotype and limestone dosage. For the higher dosage lower biomass production, lower yield of essential oil as well as the lowest content of citral (citral chemotype) and limonene (menthene chemotype) was observed. In the ocimene chemotype, no liming influence was observed on the essential oil yield and on the content of major compounds. The dosage of 3.15 g.L(-1) can be considered the best limestone dosage for the production of poejo for the experimental conditions evaluated.

  14. Application of the dating by fission tracks to determine thermicity of basins within petroleum potentialities: example of Sbaa and Ahnet-North basins located in Western Saharan platform, Algeria; Application de la datation par traces de fission a l'analyse de la thermicite de bassins a potentialites petrolieres: exemple de la cuvette de Sbaa et du bassin de l'Ahnet-Nord (plate-forme saharienne occidentale, Algerie)

    Energy Technology Data Exchange (ETDEWEB)

    Akkouche, M

    2007-05-15

    The interpretation of old fundamental research works and the results obtained by this study based on: the analysis of apatite fission tracks (AFT), the burial evolution curves and the sequential analysis of outcrop section, as well as the curves of signal of gamma ray (GR) analysis from drilling wells, allow to precise that the geodynamic evolution of the studied Algerian sedimentary basins (Ahnet and Sbaa) have been occurred principally in the Paleozoic era. The analysis of burial evolution curves shows that during the Paleozoic period, the sedimentation is controlled by the tectonic subsidence, particularly at the end of Carboniferous in the favor of Ougarta folding. The curves indicate an uplift from the Permian to middle Jurassic, expressed by an important erosion of sedimentary series and is considered as a response of thermal convection of the Saharan platform followed by thermal subsidence. This can be explained by the halt motion of rift extension caused by the opening of the Atlantic Ocean. To bring the sequential analysis out, it appears that Ahnet and Sbaa basins present contrasted stratigraphic recordings, so much in time than in space, indicated the different effects from subsidence and erosions according to their sectors. In thermal point of view, temperatures are still moderate in Sbaa depression and favorite the preservation of organic matter and might generate hydrocarbons until now. However, these conditions are not similar to ones in the case of Ahnet basin. In the North part of Ahnet basin (MRS-1, MSL-1), the ages are around 50 Ma, attested that sedimentary layers have been sustained a post-Hercynian thermal phase. This phase could be estimated probably more than 100 C. This is also produced during the regional extension of the Triassic-Jurassic rifting. This episode could also be responsible of disappearance (total?) of pre-existence fission tracks in the Devonian layers of the well MSR-1, which exhibits at the depth 505 m under the Hercynian unconformity an age FT of 100 my. In the south part, ages are respectively 37 and 26 Ma at the depths: 1030 m (MKRN-1) and 1532 m (BH-5). These suggest that Cenozoic thermal alteration of tracks is more intense than in the north of the basin and may reflect important erosion, eventually accompanied with high geothermal gradients. In conclusion, three thermal episodes in the studied area have influenced the formation and the migration of hydrocarbons and consequently their nature; Varisc orogenesis, the Atlantic opening to Jurassic and the thermal convection of the Hoggar at the Eocene age. Clearly, the latest is the most event which affects the hydrocarbons of Ahnet reservoirs as shown by the increased temperatures from the north to south of the studied area. A secondary cracking occurred certainly during Eocene transforming oil to gas. Also, the outcrop studies allow defining the deposits influenced by storms (shallow depth {<=} 100-150 m) in the sedimentary series of the Ougarta ridge and the evident deposits of Jokulhlaups in the unit IV in Kheneg Etlaia, locality (Ougarta). This can be observed distally at the Cambrian-ordovician of the well MSR-1 (Ahnet). (author)

  15. Comparative biomorphological study of the taxonomy and Phylogeny of the genera Consolida (DC.S.F. Gray and Aconitella Spach

    Directory of Open Access Journals (Sweden)

    Trifinova, V. I.

    1990-12-01

    Full Text Available Phylogenetic connections within the genus Consolida and between the genera Consolida and Aconitella have been revealed as a result of the comparison of anatomical, k.aryological and morphological data. This study has confinned the correctness or the intrageneric division of the genus Consolida proposed by E. Huth (1895, for three out or the five sections growing on the territory of the USSR. Taxonomical rank. has been specified fo r the youngest and most highly evolved group of species of the genus Aconitella, which is regarded by many botanists as beingwithin the limits of the genus Consolida. The taxonomical position of Aconitella barbata (Bunge Sojak, whose morphological rentures are intermediate between the genera Aconitella and Consolida, has been stated exactly. The main trends in the evolution or the tribe Delphineae have been identified: beginning from the primarily perennial mesophytic genus Aconitum, which is the most primitive in its anatomical, karyological and morphological features. through a series of unknown forms to the genus Delphinium;and also from the representatives of' the annual section Delphinium of the subgenus Delphinium to the genera Consolida and Aconitella.

    [ca] Les relacions filogenètiques a l'interior del gènere Consolida i entre els gèneres Consolida i Aconitella han estat posades de manifest com a resultat de la comparació de dades anatòmiques, cariològiques i morfològiques. Aquesta investigació ha confirmat la correcció de Ja divisió intragenèrica del gènere Consolida, proposada per E. Huth (1895, per a tres de les cinc seccions que creixen al territori de la U.R.S.S. El rang taxonòmic ha estat especificat per al grup més mòdem i evolutivament avançat d'espècies del gènere Aconitella. que és considerat per molts autors a l'interior dels límits del g

  16. Achievements and perspectives in biochemistry concerning anthocyanin modification for blue flower coloration.

    Science.gov (United States)

    Sasaki, Nobuhiro; Nakayama, Toru

    2015-01-01

    Genetic engineering of roses and other plants of floricultural importance to give them a truly blue petal color is arguably one of the holy grails of plant biotechnology. Toward this goal, bluish carnations and roses were previously engineered by establishing an exclusive accumulation of delphinidin (Dp)-type anthocyanins in their petals via the heterologous expression of a flavonoid 3',5'-hydroxylase gene. Very recently, purple-blue varieties of chrysanthemums were also genetically engineered via a similar biochemical strategy. Although the floral colors of these transgenic plants still lack a true blue color, the basis for the future molecular breeding of truly blue flowers is via the engineering of anthocyanin pathways. Anthocyanins with multiple aromatic acyl groups (often referred to as polyacylated anthocyanins) in the 3'- or 7-position tend to display a more stable blue color than non-acylated anthocyanins. The 7-polyacylation process during the biosynthesis of purple-blue anthocyanins in delphinium (Delphinium grandiflorum) was found to occur in vacuoles using acyl-glucose as both the glucosyl and acyl donor. Glucosyltransferases and acyltransferases involved in anthocyanin 7-polyacylation in delphinium are vacuolar acyl-glucose-dependent enzymes belonging to the glycoside hydrolase family 1 and serine carboxypeptidae-like protein family, respectively. The 7-polyacylation proceeds through the alternate glucosylation and p-hydroxybenzoylation catalyzed by these enzymes. p-Hydroxybenzoyl-glucose serves as the p-hydroxybenzoyl and glucosyl donor to produce anthocyanins modified with a p-hydroxybenzoyl-glucose concatemer at the 7-position. This novel finding has provided a potential breakthrough for the genetic engineering of truly blue flowers, where polyacylated Dp-type anthocyanins are accumulated exclusively in the petals. © The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For

  17. Plant-animal interactions in larkspur poisoning in cattle.

    Science.gov (United States)

    Ralphs, M H; Olsen, J D; Pfister, J A; Manners, G D

    1988-09-01

    Larkspur (Delphinium spp.) toxicity in cattle seriously impedes the efficient use of productive mountain rangelands. Larkspurs contain complex diterpenoid alkaloids that cause acute intoxication and death from respiratory paralysis. Alkaloids and their concentrations vary among larkspur species, plant parts and phenological growth stages, thus causing great variability in toxicity. Ingestion rate of larkspur by the cow, alkaloid toxicity and concentration in the plant and the kinetics of absorption and excretion interact to determine whether a cow is poisoned. Plant and animal factors influencing consumption and subsequent intoxication must be further elucidated to devise management strategies to reduce liverstock losses.

  18. Toru Takemitsu, novembr steps: Oppositions et complémentarités des traditions

    Directory of Open Access Journals (Sweden)

    Bosseur Jean-Yves

    2005-01-01

    Full Text Available (francuski Dans November Steps (1967 T. Takemitsu démontre des complémentarités d'idées de sources orientales et occidentales. Fondée sur le principe de variations, l'œuvre fait des références au même procédé dans une forme de musique japonaise du XVIIe s. (koto et à certaines oeuvres de Debussy (Prélude à l'après-midi d'un faune, Jeux. Les instruments traditionnels japonais (biwa et shakuhachi sont confrontés à ceux de provenance occidentale. Takemitsu cherche des points communs entre traditions occidentale et orientale, pas seulement des conflits.

  19. CHEMICAL COMPOSITION VARIABILITY IN THE Uncaria tomentosa (cat’s claw WILD POPULATION

    Directory of Open Access Journals (Sweden)

    Evelyn Maribel Condori Peñaloza

    2015-03-01

    Full Text Available Uncaria tomentosa (cat's claw is a vine widely distributed throughout the South-American rainforest. Many studies investigating the chemical composition of cat's claw have focused on the pentacyclic (POA and tetracyclic oxindole alkaloids (TOA, quinovic acid glycosides (QAG, and polyphenols (PPH. Nevertheless, it is still uncertain how environmental factors affect chemical groups. The aim of this work was to better understand the influence of environmental factors (geographic origin, altitude, and season on cat's claw chemical composition. Stem bark, branches and leaf samples were extracted and analyzed by HPLC-PDA. The data obtained were explored by multivariate analysis (HCA and PCA. Higher amounts of oxindole alkaloids and PPH were found in leaves, followed by stem bark and branches. No clear relationship was verified among geographic origin or altitude and chemical composition, which remained unchanged regardless of season (dry or rainy. However, three oxindole alkaloid chemotypes were clearly recognized: chemotype I (POA with cis D/E ring junction; chemotype II (POA with trans D/E ring junction; and chemotype III (TOA. Thus, environmental factors appear to have only a minor influence on the chemical heterogeneity of the cat's claw wild population. Nevertheless, the occurrence of different chemotypes based on alkaloid profiles seems to be clear.

  20. Phytochemical diversity of the essential oils of Mexican Oregano (Lippia graveolens Kunth) populations along an Edapho-climatic gradient.

    Science.gov (United States)

    Calvo-Irabién, Luz María; Parra-Tabla, Victor; Acosta-Arriola, Violeta; Escalante-Erosa, Fabiola; Díaz-Vera, Luciana; Dzib, Gabriel R; Peña-Rodríguez, Luis Manuel

    2014-07-01

    Mexican oregano (Lippia graveolens) is an important aromatic plant, mainly used as flavoring and usually harvested from non-cultivated populations. Mexican oregano essential oil showed important variation in the essential-oil yield and composition. The composition of the essential oils extracted by hydrodistillation from 14 wild populations of L. graveolens growing along an edaphoclimatic gradient was evaluated. Characterization of the oils by GC-FID and GC/MS analyses allowed the identification of 70 components, which accounted for 89-99% of the total oil composition. Principal component and hierarchical cluster analyses divided the essential oils into three distinct groups with contrasting oil compositions, viz., two phenolic chemotypes, with either carvacrol (C) or thymol (T) as dominant compounds (contents >75% of the total oil composition), and a non-phenolic chemotype (S) dominated by oxygenated sesquiterpenes. While Chemotype C was associated with semi-arid climate and shallower and rockier soils, Chemotype T was found for plants growing under less arid conditions and in deeper soils. The plants showing Chemotype S were more abundant in subhumid climate. High-oil-yield individuals (>3%) were identified, which additionally presented high percentages of either carvacrol or thymol; these individuals are of interest, as they could be used as parental material for scientific and commercial breeding programs.

  1. LA SABIDURIA DEL SILENCIO. HACIA EL DIÁLOGO... ¡OTROS MUNDOS SON POSIBLES!

    Directory of Open Access Journals (Sweden)

    Juan Carlos Valverde Campos

    2012-05-01

    Full Text Available A Occidentales y Orientales nos cobija un mismo techo. Sin embargo, la manera de concebir el mundo y, por ende, de actuar, es diferente. La sociedad globalizada en la que vivimos quiere hacer de este mundo uno solo, con un solo corazón y una sola manera de actuar y pensar. Occidente ha impuesto su mundo cuando en realidad son muchos y debemos conocerlos. El pensamiento oriental tiene mucho qué decirnos a los occidentales. En todo caso, el silencio que impone la escucha es fundamental en eso que tantos llaman hoy el diálogo.

  2. El derecho occidental del siglo XXI y el concepto de familia jurídica

    OpenAIRE

    María Virginia Gaviria Gil

    2013-01-01

    El objetivo de este artículo es examinar si el concepto de familia jurídica romano germánica, o las diferentes clasificaciones que se hacen de los Estados occidentales donde existe una importante influencia del derecho romano y de las codificaciones liberales del siglo XIX, y donde se utiliza la ley como principal fuente del derecho–, es adecuado para explicar los ordenamientos jurídicos occidentales del siglo XXI. Al mostrar cómo los presupuestos sobre los cuales se construyó el concepto han...

  3. The Diplomacy of the Jaguar: French Airpower in Postcolonial African Conflicts

    Science.gov (United States)

    2009-03-01

    the sympo- sium, 5 February 2007. Gaulme, François. “La politique française d’intervention dans les conflits limités en Afrique .” Penser les Ailes...colonies of the Afrique Occidentale Française and Afrique Equatoriale Française in 960. Pierre Bezbakh, Petit Larousse de l’Histoire de France, 73...related clauses. In these countries that had composed the Afrique Occidentale Française (AOF: French Occidental Africa) and the Afrique Equatoriale

  4. Experimental and Structural Testing Module to Analyze Paralogue-Specificity and Affinity in the Hsp90 Inhibitors Series

    Science.gov (United States)

    Taldone, Tony; Patel, Pallav D.; Patel, Maulik; Patel, Hardik J.; Evans, Christopher E.; Rodina, Anna; Ochiana, Stefan; Shah, Smit K.; Uddin, Mohammad; Gewirth, Daniel; Chiosis, Gabriela

    2014-01-01

    We here describe the first reported comprehensive analysis of Hsp90 paralogue affinity and selectivity in the clinical Hsp90 inhibitor chemotypes. This has been possible through the development of a versatile experimental assay based on a new FP-probe (16a) that we both describe here. The assay can test rapidly and accurately the binding affinity of all major Hsp90 chemotypes and has a testing range that spans low nanomolar to millimolar binding affinities. We couple this assay with a computational analysis that allows for rationalization of paralogue selectivity and defines not only the major binding modes that relay pan-paralogue binding or, conversely, paralogue selectivity, but also identifies molecular characteristics that impart such features. The methods developed here provide a blueprint for parsing out the contribution of the four Hsp90 paralogues to the perceived biological activity with the current Hsp90 chemotypes and set the ground for the development of paralogue selective inhibitors. PMID:23965125

  5. The essential oil of Lippia alba: analysis of samples from French overseas departments and review of previous works.

    Science.gov (United States)

    Hennebelle, Thierry; Sahpaz, Sevser; Dermont, Charles; Joseph, Henry; Bailleul, François

    2006-10-01

    This paper contains new data on the chemical composition of the essential oil of Lippia alba (Mill.) N. E. Brown, as well as an overview of the available literature. Although the composition of the essential oil of this species is well-documented, discrepancies between the reported results suggest that many chemotypes and morphotypes exist. The analysis of essential oils obtained from the leaves of samples from three different locations in the French Overseas Departments (French Guiana, Martinique, and two different places in Guadeloupe) have shown that the composition of each one is quite different. This new data, along with a review and discussion of published studies, enabled us to establish seven distinct chemotypes. Possible connections between these chemotypes and morphotypes already described were also investigated.

  6. Diterpenoid alkaloid toxicosis in cattle in the Swiss Alps.

    Science.gov (United States)

    Puschner, Birgit; Booth, Marcia C; Tor, Elizabeth R; Odermatt, Arnold

    2002-02-01

    Between 1995 and 1999, several cattle of a group of 80 heifers died acutely on a pasture in the Swiss Alps. The animals were Found dead between July 9th and 15th eachyear. Only 1 animal was examined on post-mortem, and no significant lesions were found. Aconitum vulpera, A napellus, and Delphinium elatum were identified in the pasture. The presence of diterpenoid alkaloid-containing plants in the pasture, the rapid death of the animals, and the lack of pathologic lesions suggested diterpenoid alkaloid toxicosis as a cause of death. A multiresidue alkaloid screen using gas chromatography with a mass spectrometric detector was employed on rumen, abomasal, small intestine, and cecal contents from the I heifer. Deltaline, deltamine, and lycoctonine were identified. Aconitine was found in all gastrointestinal samples using a sensitive and highly specific liquid chromatography/mass spectrometry methodology for aconitine analysis. The findings ofditerpenoid alkaloids in the gastrointestinal contents confirmed exposure to Delphinium and Aconitum spp, possibly resulting in sudden death.

  7. The Role of Acyl-Glucose in Anthocyanin Modifications

    Directory of Open Access Journals (Sweden)

    Nobuhiro Sasaki

    2014-11-01

    Full Text Available Higher plants can produce a wide variety of anthocyanin molecules through modification of the six common anthocyanin aglycons that they present. Thus, hydrophilic anthocyanin molecules can be formed and stabilized by glycosylation and acylation. Two types of glycosyltransferase (GT and acyltransferase (AT have been identified, namely cytoplasmic GT and AT and vacuolar GT and AT. Cytoplasmic GT and AT utilize UDP-sugar and acyl-CoA as donor molecules, respectively, whereas both vacuolar GT and AT use acyl-glucoses as donor molecules. In carnation plants, vacuolar GT uses aromatic acyl-glucoses as the glucose donor in vivo; independently, vacuolar AT uses malylglucose, an aliphatic acyl-glucose, as the acyl-donor. In delphinium and Arabidopsis, p-hydroxybenzoylglucose and sinapoylglucose are used in vivo as bi-functional donor molecules by vacuolar GT and AT, respectively. The evolution of these enzymes has allowed delphinium and Arabidopsis to utilize unique donor molecules for production of highly modified anthocyanins.

  8. Chemogeography and antimicrobial activity of essential oils from Geijera parviflora and Geijera salicifolia (Rutaceae): two traditional Australian medicinal plants.

    Science.gov (United States)

    Sadgrove, Nicholas J; Gonçalves-Martins, Maximilien; Jones, Graham L

    2014-08-01

    Essential oils were hydrodistilled from 27 specimens of Geijera parviflora Lindl., (Rutaceae) and nine specimens of Geijera salicifolia Schott, collected over a wide geographic range in New South Wales, Queensland and South Australia. Essential oils were produced by traditional hydrodistillation and characterised using GC-MS. From one specimen a serendipitous discovery was made of bioactive coumarins dissolved in the hydrosol, which were the coumarins isopsoralen, xanthyletine and osthole. These coumarins were not present in the essential oil from that specimen. Using essential oil composition from all specimens, principal component analysis (PCA) demonstrated nine clusters for G. parviflora and three for G. salicifolia. Some clusters are representative of previously described chemotypes and some are reflective of possible chemotypes requiring more comprehensive sampling for confirmation. Thus, another three or four possible chemotypes of G. parviflora and one of G. salicifolia have been tentatively identified. Using micro-titre plate broth dilution assays, antibacterial and antifungal activity of all chemotypes was investigated. In this regard, the 'green oil' chemotype, restricted to G. parviflora, with major components linalool, geijerene/pregeijerene, 1,8-cineol and bicyclogermacrene, demonstrated the highest antimicrobial and free radical scavenging activity. Thus, in the light of traditional use reports of local analgaesia and bioactivity demonstrated in the current study, oils from select chemotypes of G. parviflora may be useful in suitably compounded lotions and creams designed for topical antimicrobial applications and local pain relief. In addition, because major components are known for insecticidal activities, such lotions may also be useful as topically applied insect repellents.

  9. Synthesis and Antiangiogenic Activity of N-Alkylated Levamisole Derivatives

    DEFF Research Database (Denmark)

    Hansen, Anders N.; Bendiksen, Christine D.; Sylvest, Lene

    2012-01-01

    less effective than antibody treatment and are also associated with serious side effects. The discovery of new chemotypes with efficient antiangiogenic activity is therefore of pertinent interest. (S)-Levamisole hydrochloride, an anthelminthic drug approved for human use and with a known clinical......-culture with normal human dermal fibroblasts. Interestingly, the cluster morphology caused by N-methyllevamisole was different than the clusters observed for levamisole, and a third "cord-like" morphology resembling that of the known drug suramin was observed for an aniline-containing derivative. New chemotypes...

  10. Molecular characterization of ochratoxin A producing strains of the genus Penicillium

    DEFF Research Database (Denmark)

    Castella, G.; Larsen, Thomas Ostenfeld; Cabanes, J.;

    2002-01-01

    the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate...... the chemotype of P. nordicum and strains from group II that of P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either P. verrucosum or P. nordicum. The homolgy between...

  11. Macrocyclic prolinyl acyl guanidines as inhibitors of β-secretase (BACE).

    Science.gov (United States)

    Boy, Kenneth M; Guernon, Jason M; Wu, Yong-Jin; Zhang, Yunhui; Shi, Joe; Zhai, Weixu; Zhu, Shirong; Gerritz, Samuel W; Toyn, Jeremy H; Meredith, Jere E; Barten, Donna M; Burton, Catherine R; Albright, Charles F; Good, Andrew C; Grace, James E; Lentz, Kimberley A; Olson, Richard E; Macor, John E; Thompson, Lorin A

    2015-11-15

    The synthesis, evaluation, and structure-activity relationships of a class of acyl guanidines which inhibit the BACE-1 enzyme are presented. The prolinyl acyl guanidine chemotype (7c), unlike compounds of the parent isothiazole chemotype (1), yielded compounds with good agreement between their enzymatic and cellular potency as well as a reduced susceptibility to P-gp efflux. Further improvements in potency and P-gp ratio were realized via a macrocyclization strategy. The in vivo profile in wild-type mice and P-gp effects for the macrocyclic analog 21c is presented.

  12. HPLC-DAD-MS identification of bioactive secondary metabolites from Ferula communis roots.

    Science.gov (United States)

    Arnoldi, Lolita; Ballero, Mauro; Fuzzati, Nicola; Maxia, Andrea; Mercalli, Enrico; Pagni, Luca

    2004-06-01

    A simple HPLC method was developed to distinguish between 'poisonous' and 'non-poisonous' chemotypes of Ferula communis. The method was performed on a C8 reverse phase analytical column using a binary eluent (aqueous TFA 0.01%-TFA 0.01% in acetonitrile) under gradient condition. The two chemotypes showed different fingerprints. The identification of five coumarins and eleven daucane derivatives by HPLC-diode array detection (HPLC-DAD) and HPLC-MS is described. A coumarin, not yet described, was detected.

  13. Smallholder Information Sources and Communication Pathways for Cashew Production and Marketing in Tanzania: An Ex-Post Study in Tandahimba and Lindi Rural Districts, Southern Tanzania

    Science.gov (United States)

    Nyambo, Brigitte; Ligate, Elly

    2013-01-01

    Purpose: To identify and review production and marketing information sources and flows for smallholder cashew (Anacardium occidentale L.) growers in Tanzania and recommend systems improvements for better technology uptake. Design/methodology/approach: Two-stage purposive samples were drawn. First, two districts in the main cashew producing areas,…

  14. Cashew Nut Allergy in Children

    NARCIS (Netherlands)

    J.P.M. Kuiper- van der Valk (Hanna)

    2016-01-01

    markdownabstractThe content of the thesis contributes to the knowledge of the cashew nut and cashew nut allergy. Cashew nut allergy is an important healthcare problem, especially in children. The cashew nut (Anacardium occidentale) belongs to the Ancardiaceae family and the major allergen components

  15. The Associate Program on Ethnobiology, Socio-Economic Value Assessment and Community Based Conservation

    Science.gov (United States)

    2000-10-01

    petandra (Silk cotton), Dracaena deisteliana (Peace plant) or from uprooting cultural plants - Raphia vinifera (Raffia palm), Elaeis guineensis ( oil palm...Agave sisalana Leaves, Stems Ageratum conyzoides Whole plant Allanblackia Seeds Aloe Vera Leaves Alstonia congensis Barks, latex Anacardium occidentale...medicinal plants for treatment. Common spices and herbs, however, are obtained from herb sellers. These: Aframomum sp, Capsicum sp, Piper sp, castor oil

  16. Reducing losses inflicted by insect pests on cashew, using weaver ants as a biological control agent

    DEFF Research Database (Denmark)

    Anato, Florence; Wargui, Rosine; Sinzogan, Antonio

    2015-01-01

    BACKGROUND: Cashew (Anacardium occidentale Linnaeus) is the largest agricultural export product in Benin. However, yields and quality are lost due to inefficient pest control. Weaver ants (Oecophylla spp.) may control pests in this crop as they eat and deter pests. In Benin, cashew pest damages...

  17. Inhibitory effect of some natural and semisynthetic phenolic lipids upon acetylcholinesterase activity.

    Science.gov (United States)

    Stasiuk, Maria; Bartosiewicz, Dominika; Kozubek, Arkadiusz

    2008-06-01

    The effect of phenolic lipids isolated from rye grains and cashew nut shell liquid (CNSL) from Anacardium occidentale and their semisynthetic derivatives on erythrocyte ghost's acetylcholinesterase activity was studied. It has been shown that all tested compounds decreased the enzymatic activity of acetylcholinesterase. This effect depends on the type of studied compounds. Three of them completely inhibit acetylcholinesterase activity at the micromolar concentration.

  18. Secondary metabolite comparison of the species within the Heterobasidion annosum s.l. complex

    DEFF Research Database (Denmark)

    Hansson, David; Wubshet, Sileshi Gizachew; Olson, Åke

    2014-01-01

    .s. and H. irregulare are pine infecting species, and H. parviporum, H. occidentale and H. abietinum are non-pine infecting. Principal component analysis (PCA) of the LC–HRMS data showed that samples from the five species could be separated into five groups and in accordance with the differences in host...

  19. Diasporicità sull’esempio di Erich Auerbach in Gad Lerner e Miro Silvera

    NARCIS (Netherlands)

    Jansen, M.M.; Arts, Clemens

    2011-01-01

    A partire dall’esilio di Erich Auerbach a Istanbul dal 1936 al 1947, il contributo propone una riflessione sui concetti di diaspora e di esilio da adattare a due scrittori italo‐ebrei provenienti dalla diaspora orientale: Miro Silvera con Il passeggero occidentale (2009) e Gad Lerner con Scintille

  20. I fondamenti religiosi della finanza islamica

    Directory of Open Access Journals (Sweden)

    Maria d'Arienzo

    2012-07-01

    SOMMARIO: 1. Religione ed economia nella visione occidentale e in quella musulmana – 2. Principi religiosi del sistema economico islamico – 3. Connotazione specificamente religiosa della finanza islamica – 4. Finanza “etica”, finanza “islamica” e “etica economica”.

  1. Smallholder Information Sources and Communication Pathways for Cashew Production and Marketing in Tanzania: An Ex-Post Study in Tandahimba and Lindi Rural Districts, Southern Tanzania

    Science.gov (United States)

    Nyambo, Brigitte; Ligate, Elly

    2013-01-01

    Purpose: To identify and review production and marketing information sources and flows for smallholder cashew (Anacardium occidentale L.) growers in Tanzania and recommend systems improvements for better technology uptake. Design/methodology/approach: Two-stage purposive samples were drawn. First, two districts in the main cashew producing areas,…

  2. Diasporicità sull’esempio di Erich Auerbach in Gad Lerner e Miro Silvera

    NARCIS (Netherlands)

    Jansen, M.M.; Arts, Clemens

    2011-01-01

    A partire dall’esilio di Erich Auerbach a Istanbul dal 1936 al 1947, il contributo propone una riflessione sui concetti di diaspora e di esilio da adattare a due scrittori italo‐ebrei provenienti dalla diaspora orientale: Miro Silvera con Il passeggero occidentale (2009) e Gad Lerner con Scintille (

  3. La cultura della simultaneità nel Taijiquan and Qigong Day

    Directory of Open Access Journals (Sweden)

    Sergio Raimondo

    2009-07-01

    Full Text Available Il crescente successo che il taijiquan e il qigong - discipline psicofisiche di origine cinese, popolari soprattutto in quanto tecniche di longevità - riscuotono tra la popolazione occidentale costituisce un fenomeno interessante per le scienze sociali soprattutto in quanto culla di peculiari scambi culturali nel contesto della globalizzazione.

  4. Análisis de elementos gráficos de contenido occidental en los glifos de los códices coloniales del centro de México: el caso de los antropónimos castellanos

    National Research Council Canada - National Science Library

    Juan José Batalla Rosado

    2015-01-01

    ... culturales occidentales. Palabras clave: Códices mesoamericanos, escritura jeroglífica náhuatl, cultura azteca. Analysis of Western Graphics Elements in the Glyphs of Colonial Central Mexican Codices: The Case of Spanish Anthroponyms ABSTRACT In this text, we present the study of Spanish anthroponyms containing «Western» elements. We analyze the «l...

  5. Reducing losses inflicted by insect pests on cashew, using weaver ants as a biological control agent

    DEFF Research Database (Denmark)

    Anato, Florence; Wargui, Rosine; Sinzogan, Antonio

    2015-01-01

    BACKGROUND: Cashew (Anacardium occidentale Linnaeus) is the largest agricultural export product in Benin. However, yields and quality are lost due to inefficient pest control. Weaver ants (Oecophylla spp.) may control pests in this crop as they eat and deter pests. In Benin, cashew pest damages, ...

  6. Synthesis and biological evaluation of new salicylate macrolactones from anacardic acids

    Energy Technology Data Exchange (ETDEWEB)

    Logrado, Lucio P.L.; Santos, Maria Lucilia dos [Brasilia Univ., DF (Brazil). Inst. de Quimica. Lab. de Isolamento e Transformacao de Moleculas Organicas]. E-mail: mlsantos@unb.br; Silveira, Damaris [Brasilia Univ., DF (Brazil). Faculdade de Ciencias da Saude; Romeiro, Luiz A.S. [Universidade Catolica de Brasilia, Taguatinga, DF (Brazil). Nucleo de Quimica Bioorganica e Medicinal; Moraes, Manoel O. de; Cavalcanti, Bruno C.; Costa-Lotufo, Leticia V.; Pessoa, Claudia do O [Ceara Univ., Fortaleza, CE (Brazil). Lab. de Oncologia Experimental

    2005-11-15

    onnection with our ongoing investigation in the search for new bioactive compounds using non-isoprenoid phenolic lipids from Anacardium occidentale as starting material, we describe the synthesis and cytotoxicity screening of some novel salicylate macrolactones prepared from anacardic acids, the major constituents of natural cashew nut-shell liquid (CNSL). (author)

  7. Oecophylla longinoda (Hymenoptera

    DEFF Research Database (Denmark)

    Anato, F. M.; Sinzogan, A. A. C.; Offenberg, J.

    2017-01-01

    Weaver ants, Oecophylla spp., are known to positively affect cashew, Anacardium occidentale L., raw nut yield, but their effects on the kernels have not been reported. We compared nut size and the proportion of marketable kernels between raw nuts collected from trees with and without ants. Raw nu...

  8. Diasporicità sull’esempio di Erich Auerbach in Gad Lerner e Miro Silvera

    NARCIS (Netherlands)

    Jansen, M.M.; Arts, Clemens

    2011-01-01

    A partire dall’esilio di Erich Auerbach a Istanbul dal 1936 al 1947, il contributo propone una riflessione sui concetti di diaspora e di esilio da adattare a due scrittori italo‐ebrei provenienti dalla diaspora orientale: Miro Silvera con Il passeggero occidentale (2009) e Gad Lerner con Scintille (

  9. Paola Di Gennaro, Wandering through Guilt. The Cain Archetype in the Twentieth-Century Novel

    Directory of Open Access Journals (Sweden)

    Saverio Vita

    2016-11-01

    Full Text Available I temi della colpa e dell’esilio, del vagabondaggio sono condivisi non solo nell’ambito limitato della cultura occidentale. Paola Di Gennaro lo dimostra, insieme a molti altri aspetti rilevanti, in questo suo libro che vuole analizzare l’attore archetipico dei due temi: Caino.

  10. Stirner: a wanderer of the spirit

    Directory of Open Access Journals (Sweden)

    Michele Mosca

    2013-11-01

    Full Text Available Stirner, ancor prima di Nietzsche, ha di fatto aperto una crepa all’interno di quella tradizione metafisica occidentale che ha sempre posto la vita come qualcosa che deve essere giudicato, compreso e misurato, che ha fatto del sapere e della conoscenza gli strumenti per accedere

  11. Classi plurilingue in condizioni di super-diversita

    NARCIS (Netherlands)

    Spotti, Max

    2015-01-01

    Il plurilinguismo nelle classi è e rimane un punto di preoccupazione per gli insegnanti fin dall'inizio della scuola dell'obbligo. In Italia, cosi come in tutta l’Europa occidentale, da quando le scuole hanno aperto le loro porte ai bambini appartenenti a comunità immigrate, i docenti si sono dovuti

  12. African Journal of Biotechnology - Vol 10, No 32 (2011)

    African Journals Online (AJOL)

    Genetic diversity and relationships among cabbage (Brassica oleracea var. capitata) ... Seed germination and in vitro regeneration of the African medicinal and ... Passive- and active-cigarette smoking: Effects on the levels of antioxidant vitamins ... extracts of three medicinal plants (Anacardium occidentale, Psidium guajava ...

  13. Influência de diversos derivados de vegetais na sobrevida das larvas de Aedes fluviatilis(Lutz (Diptera: Culicidade em laboratorio Larvicidal properties of plant extracts against Aedes fluviatilis (Lutz (Diptera: Culicidae in the laboratory

    Directory of Open Access Journals (Sweden)

    Rotraut A. G. B. Consoli

    1988-03-01

    Full Text Available As propriedades larvicidas de 34 extratos, provenientes de 29 vegetais, foram testados em larvas de Aedes fluviatilis (Lutz (Diptera: Culicidae nas concentrações de 100, 10 e 1 ppm. 26,5% dos exames utilizados, reduziram significamente a sobrevida larvária (alfa = 0,05, quando empregados na concentração de 100 ppm (Anacardium occidentale, Agave americana, Allium sativum, Coriandrum sativum, Nerium oleander, Spatodea campanulata, Tibouchina scrobiculata e Vernonia salzmanni. O ácido anacárdio (A. occidentale mostrou-se larvicida na concentração de 10 ppm e o extrato bruto de A. sativum foi eficaz contra as larvas na concentração de 1 ppm.The larvicidal properties of 34 plant extracts were tested against Aedes fluviatilis (Lutz (Diptera: Culicidae larvae, at 100, 10 and 1 ppm concentrations; 26,6% of the extracts enhanced larval mortality (x = 0,05 at 100 ppm (Anacardium occidentale, Agave americana, Allium sativum, Coriandrum sativum, Nerium oleander, Spatodea campanulata, Tibouchina serobiculata and Vernonia salzmanni. Anacardic acid (A. occidentale was effective at 10 ppm and A. sativum (crude extract at 1 ppm.

  14. Bangalore, ville des nouvelles technologies

    Directory of Open Access Journals (Sweden)

    Clarisse Didelon

    2003-06-01

    Full Text Available Bangalore est devenue la Silicon Valley de l’Inde. Une partie de ses habitants y vit à l’occidentale mais le reste de la population souffre de la croissance spectaculaire de la ville. Face à l’insuffisance des infrastructures, Bangalore devient de moins en moins attractive pour les entreprises internationales.

  15. Phenotypic Variations in the Foliar Chemical Profile of Persea americana Mill. cv. Hass.

    Science.gov (United States)

    García-Rodríguez, Yolanda Magdalena; Torres-Gurrola, Guadalupe; Meléndez-González, Claudio; Espinosa-García, Francisco J

    2016-12-01

    The Hass avocado tree Persea americana cv. Hass was derived from a single hybrid tree of P. americana var. drymifolia and P. americana var. guatemalensis, and it is propagated clonally by grafting. This cultivar is the most widely planted in the world but its profile of secondary metabolites has been studied rarely despite of its importance in plant protection. We illustrate the variability of the volatilome of mature leaves by describing the average chemical composition and the phenotypic variability found in 70 trees. Contrary to the uniformity expected in the Hass cultivar, high variability coefficients were found for most of the 36 detected foliar volatile compounds; furthermore we found six chemotypes grouping the foliar phenotypes of the sampled trees using hierarchical cluster analysis. About 48% of trees were grouped in one chemotype; five chemotypes grouped the remaining trees. The compounds that determined these chemotypes were: estragole, α-farnesene, β-caryophyllene, germacrene D, α-cubebene and eugenol. This striking variation in a cultivar propagated clonally is discussed in terms of somatic mutation.

  16. Discovery and SAR of a novel series of non-MPEP site mGlu₅ PAMs based on an aryl glycine sulfonamide scaffold.

    Science.gov (United States)

    Rodriguez, Alice L; Zhou, Ya; Williams, Richard; Weaver, C David; Vinson, Paige N; Dawson, Eric S; Steckler, Thomas; Lavreysen, Hilde; Mackie, Claire; Bartolomé, José M; Macdonald, Gregor J; Daniels, J Scott; Niswender, Colleen M; Jones, Carrie K; Conn, P Jeffrey; Lindsley, Craig W; Stauffer, Shaun R

    2012-12-15

    Herein we report the discovery and SAR of a novel series of non-MPEP site metabotropic glutamate receptor 5 (mGlu(5)) positive allosteric modulators (PAMs) based on an aryl glycine sulfonamide scaffold. This series represents a rare non-MPEP site mGlu(5) PAM chemotype.

  17. epi-Aszonalenins A, B, and C from Aspergillus novofumigatus

    DEFF Research Database (Denmark)

    Rank, Christian; Phipps, Richard Kerry; Harris, Pernille

    2006-01-01

    Three new benzodiazepines have been isolated from an unusual chemotype of Aspergillus novofumigatus: epi-aszonalenins A, B, and C. The structures were elucidated by use of one- and two-dimensional NMR spectroscopic techniques and HR ESI MS. The relative configuration was established on the basis...

  18. Review of the traditional uses, phytochemistry, pharmacology and toxicology of giant fennel (Ferula communis L. subsp. communis

    Directory of Open Access Journals (Sweden)

    Maryam Akaberi

    2015-11-01

    Full Text Available Ferula communis L., subsp. communis, namely giant fennel, has extensively been used in traditional medicine for a wide range of ailments. Fresh plant materials, crude extracts and isolated components of F. communis showed a wide spectrum of in vitro and in vivo pharmacological properties including antidiabetic, antimicrobial, antiproliferative, and cytotoxic activities. The present paper, reviews the traditional uses, botany, phytochemistry, pharmacology and toxicology of F. communis in order to reveal its therapeutic potential and future research opportunities. A bibliographic literature search was conducted in different scientific databases and search engines including Scopus, Cochrane Library, Embase, Google Scholar, Pubmed, SciFinder, and Web of science. Phytochemical studies have led to the isolation of different compounds such as sesquiterpenes from F. communis. This plant has two different chemotypes, the poisonous and non-poisonous chemotypes. Each chemotype is endowed with various constituents and different activities. The poisonous chemotype exhibits anticoagulant and cytotoxic activities with sesquiterpene coumarins as major constituents, while the non-poisonous one exhibits estrogenic and cytotoxic effects with daucane sesquiterpene esters as the main compounds. In addition, although various pharmacological properties have been reported for F. communis, anti-microbial activities of the plant have been investigated in most studies. Studies revealed that F. communis exhibits different biological activities, and contains various bioactive compounds. Although, antibacterial and cytotoxic activities are the two main pharmacological effects of this plant, further studies should focus on the mechanisms underlying these actions, as well as on those biological activities that have been reported traditionally.

  19. Identification of novel HDAC inhibitors through cell based screening and their evaluation as potential anticancer agents.

    Science.gov (United States)

    Wang, Tong; Sepulveda, Mario; Gonzales, Paul; Gately, Stephen

    2013-09-01

    A series of benzimidazole based HDAC inhibitors have been rationally designed, synthesized and screened. The SAR of this new chemotype is described. The lead compound, 11e, showed strong activity in several cellular assays and demonstrated in vivo efficacy in mouse xenograft pancreatic cancer models.

  20. Antifungal effect of various essential oils against Candida albicans. Potentiation of antifungal action of amphotericin B by essential oil from Thymus vulgaris.

    Science.gov (United States)

    Giordani, R; Regli, P; Kaloustian, J; Mikaïl, C; Abou, L; Portugal, H

    2004-12-01

    The antifungal effect of the essential oil from Satureja montana L., Lavandula angustifolia Mill., Lavandula hybrida Reverchon, Syzygium aromaticum (L.) Merril and Perry, Origanum vulgare L., Rosmarinus officinalis L. and six chemotypes of Thymus vulgaris L. on Candida albicans growth were studied. The most efficiency was obtained with the essential oil from Thymus vulgaris thymol chemotype (MIC 80% = 0.016 microL/mL and Kaff = 296 microL/mL). The presence in the culture medium of essential oil from Thymus vulgaris thymol chemotype (0.01, 0.1, 0.2, 0.3 microg/mL) and amphotericin B involved a decrease of the MIC 80% of amphotericin B. In contrast, the combination of amphotericin B and low concentrations (0.00031-0.0025 microg/mL) of essential oil was antagonistic. The strongest decrease (48%) of the MIC 80% was obtained with medium containing 0.2 microL/mL of essential oil. These results signify that the essential oil of Thymus vulgaris thymol chemotype potentiates the antifungal action of amphotericin B suggesting a possible utilization of this essential oil in addition to antifungal drugs for the treatment of mycoses.

  1. Chemical variation in the lichen genus Letrouitia (Ascomycota, Letrouitiaceae)

    DEFF Research Database (Denmark)

    Johansson, S.; Søchting, Ulrik; Elix, J.A.;

    2005-01-01

    -chlorofallacinal and 7-chloroparietinic acid were present. Eight previously unknown lichen substances were identified. A chemotype containing seven new dibenzofurans (8-chlorodioxocondidymic acid, 8-chlorodioxodidymic acid, 8-chloroxodidymic acid, dioxocondidymic acid, dioxodidymic acid, letrouitic acid....... The similarity in secondary chemistry between Letrouitiaceae and Teloschistaceae is not particularly strong, as the shared compounds are also known to occur in several other lichen families....

  2. Piperazine scaffolds via isocyanide-based multicomponent reactions

    NARCIS (Netherlands)

    Dömling, Alexander; Huang, Yijun

    2010-01-01

    Piperazine scaffolds are amongst the most extensively used backbones in medicinal chemistry and many bioactive compounds are built upon this template. The physicochemical properties and the three-dimensional structures of the different piperazine chemotypes are of utmost importance to understanding

  3. Taming THC: potential cannabis synergy and phytocannabinoid-terpenoid entourage effects

    OpenAIRE

    Russo, Ethan B.

    2011-01-01

    Tetrahydrocannabinol (THC) has been the primary focus of cannabis research since 1964, when Raphael Mechoulam isolated and synthesized it. More recently, the synergistic contributions of cannabidiol to cannabis pharmacology and analgesia have been scientifically demonstrated. Other phytocannabinoids, including tetrahydrocannabivarin, cannabigerol and cannabichromene, exert additional effects of therapeutic interest. Innovative conventional plant breeding has yielded cannabis chemotypes expres...

  4. Multilocus Genotyping and Molecular Phylogenetics Resolve a Novel Head Blight Pathogen within the Fusarium graminearum Species Complex from Ethiopia

    Science.gov (United States)

    A survey of Fusarium head blight (FHB)-contaminated wheat in Ethiopia recovered 31 isolates resembling members of the Fusarium graminearum species complex. Results of a multilocus genotyping (MLGT) assay for FHB species and trichothecene chemotype determination suggested that 22 of these isolates m...

  5. Detection of high levels of pyrrolizidine-N-oxides in the endangered plant cryptantha crassipes (Terlingua creek cat's-eye)using HPLC-ESI-MS

    Science.gov (United States)

    Chemical investigation of nine species of Cryptantha identified at least two chemotypes within the genus. It was determined that under especially harsh and dry growing conditions of the Chihuahuah desert of Texas, the N-oxides of two major pyrrolizidine alkaloids, lycopsamine and intermedine, foun...

  6. Diversity of pyrrolizidine alkaloids in native and invasive Senecio pterophorus (Asteraceae): implications for toxicity.

    Science.gov (United States)

    Castells, Eva; Mulder, Patrick P J; Pérez-Trujillo, Míriam

    2014-12-01

    Changes in plant chemical defenses after invasion could have consequences on the invaded ecosystems by modifying the interactions between plants and herbivores and facilitating invasion success. However, no comprehensive biogeographical studies have yet determined the phenotypic levels of plant chemical defenses, as consumed by local herbivores, covering large distributional areas of a species. Senecio pterophorus is a perennial shrub native to Eastern South Africa, expanded into Western South Africa and introduced into Australia and Europe. As other Asteraceae, S. pterophorus contains pyrrolizidine alkaloids (PAs) toxic to vertebrate and invertebrate herbivores. Here we analyzed S. pterophorus PAs by LC-MS/MS on foliage sampled across its entire distributional range, including the native and all non-native areas. PA concentrations and diversity was very high: we found 57 compounds belonging to 6 distinct necine base-types, including the highly toxic 1,2-unsaturated PAs (retronecine and otonecines) and the less toxic 1,2-saturated PAs (platynecine and rosmarinecines). Plants from different origins diverged in their PA absolute and relative concentrations. Rosmarinine was the most abundant compound in Australia and South Africa, but it was nearly absent in Europe. We characterized three plant chemotypes: retrorsine-senkirkine chemotype in Eastern South Africa, rosmarinine chemotype in Australia and Western South Africa, and acetylseneciphylline chemotype in Europe. PA absolute concentrations were highest in Australia. The increased absolute and relative concentrations of retronecine PAs from Australia and Europe, respectively, indicate that S. pterophorus is potentially more toxic in the invasive range than in the native range.

  7. A Comparative Analysis of the Chemical Composition, Anti-Inflammatory, and Antinociceptive Effects of the Essential Oils from Three Species of Mentha Cultivated in Romania.

    Science.gov (United States)

    Mogosan, Cristina; Vostinaru, Oliviu; Oprean, Radu; Heghes, Codruta; Filip, Lorena; Balica, Georgeta; Moldovan, Radu Ioan

    2017-02-10

    This work was aimed at correlating the chemotype of three Mentha species cultivated in Romania with an in vivo study of the anti-inflammatory and antinociceptive effects of essential oils. The selected species were Mentha piperita L. var. pallescens (white peppermint), Mentha spicata L. subsp. crispata (spearmint), and Mentha suaveolens Ehrh. (pineapple mint). Qualitative and quantitative analysis of the essential oils isolated from the selected Mentha species was performed by gas chromatography coupled with mass spectrometry (GC-MS). The anti-inflammatory activity of the essential oils was determined by the rat paw edema test induced by λ-carrageenan. The antinociceptive effect of the essential oils was evaluated by the writhing test in mice, using 1% (v/v) acetic acid solution administered intraperitonealy and by the hot plate test in mice. The results showed a menthol chemotype for M. piperita pallescens, a carvone chemotype for M. spicata, and a piperitenone oxide chemotype for M. suaveolens. The essential oil from M. spicata L. (EOMSP) produced statistically significant and dose-dependent anti-inflammatory and antinociceptive effects.

  8. A Comparative Analysis of the Chemical Composition, Anti-Inflammatory, and Antinociceptive Effects of the Essential Oils from Three Species of Mentha Cultivated in Romania

    Directory of Open Access Journals (Sweden)

    Cristina Mogosan

    2017-02-01

    Full Text Available This work was aimed at correlating the chemotype of three Mentha species cultivated in Romania with an in vivo study of the anti-inflammatory and antinociceptive effects of essential oils. The selected species were Mentha piperita L. var. pallescens (white peppermint, Mentha spicata L. subsp. crispata (spearmint, and Mentha suaveolens Ehrh. (pineapple mint. Qualitative and quantitative analysis of the essential oils isolated from the selected Mentha species was performed by gas chromatography coupled with mass spectrometry (GC-MS. The anti-inflammatory activity of the essential oils was determined by the rat paw edema test induced by λ-carrageenan. The antinociceptive effect of the essential oils was evaluated by the writhing test in mice, using 1% (v/v acetic acid solution administered intraperitonealy and by the hot plate test in mice. The results showed a menthol chemotype for M. piperita pallescens, a carvone chemotype for M. spicata, and a piperitenone oxide chemotype for M. suaveolens. The essential oil from M. spicata L. (EOMSP produced statistically significant and dose-dependent anti-inflammatory and antinociceptive effects.

  9. Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

    Directory of Open Access Journals (Sweden)

    Jianbo Qiu

    2014-08-01

    Full Text Available Members of the Fusarium graminearum species complex (FGSC are important pathogens on wheat, maize, barley, and rice in China. Harvested grains are often contaminated by mycotoxins, such as the trichothecene nivalenol (NIV and deoxynivalenol (DON and the estrogenic mycotoxin zearalenone (ZEN, which is a big threat to humans and animals. In this study, 97 isolates were collected from maize, wheat, and rice in Jiangsu and Anhui provinces in 2013 and characterized by species- and chemotype-specific PCR. F. graminearum sensu stricto (s. str. was predominant on maize, while most of the isolates collected from rice and wheat were identified as F. asiaticum. Fusarium isolates from three hosts varied in trichothecene chemotypes. The 3-acetyldeoxynivalenol (3ADON chemotype predominated on wheat and rice population, while 15ADON was prevailing in the remaining isolates. Sequence analysis of the translation elongation factor 1α and trichodiene synthase indicated the accuracy of the above conclusion. Additionally, phylogenetic analysis suggested four groups with strong correlation with species, chemotype, and host. These isolates were also evaluated for their sensitivity to carbendazim and mycotoxins production. The maize population was less sensitive than the other two. The DON levels were similar in three populations, while those isolates on maize produced more ZEN. More DON was produced in carbendazim resistant strains than sensitive ones, but it seemed that carbendazim resistance had no effect on ZEN production in wheat culture.

  10. Characterization of Stachybotrys from water-damaged buildings based on morphology, growth, and metabolite production.

    Science.gov (United States)

    Andersen, Birgitte; Nielsen, Kristian F; Jarvis, Bruce B

    2002-01-01

    Stachybotrys was found to be associated with idiopathic pulmonary hemorrhage in infants in Cleveland, Ohio. Since that time, considerable effort has been put into finding the toxic components responsible for the disease. The name Stachybotrys chartarum has been applied to most of these isolates, but inconsistent toxicity results and taxonomic confusion prompted the present study. In this study, 122 Stachybotrys isolates, mainly from water-damaged buildings, were characterized and identified by combining three different approaches: morphology, colony characteristics, and metabolite production. Two different Stachybotrys taxa, S. chartarum and one undescribed species, were found in water-damaged buildings regardless of whether the buildings were in Denmark, Finland, or the USA. Furthermore, two chemotypes could be distinguished in S. chartarum. One chemotype produced atranones, whereas the other was a macrocyclic trichothecene-producer. The second undescribed taxon produced atranones and could be differentiated from S. chartarum by its growth characteristics and pigment production. Our results correlate with different inflammatory and toxicological properties reported for these same isolates and show that the three taxa/chemotypes should be treated separately. The co-occurrence of these three taxa/chemotypes in water-damaged buildings explains the inconsistent results in the literature concerning toxicity of Stachybotrys isolated from that environment.

  11. Stachybotrys: An unusual mold associated with water-damaged buildings.

    Science.gov (United States)

    Jarvis, B; Hinkley, S; Nielsen, K

    2000-03-01

    Chemical analyses of extracts of cultures ofS. chartarum show that this fungus has two chemotypes: producers of the potent cytotoxic macrocyclic trichothecenes (e. g. satratoxins) and those that produce the diterpenoid atranones and the simple trichothecenes, trichodermol and trichodermin. All isolates ofS. chartarum produce the immunosuppressant spirocyclic drimanes.

  12. Starch Granule Re-Structuring by Starch Branching Enzyme and Glucan Water Dikinase Modulation Affects Caryopsis Physiology and Metabolism

    DEFF Research Database (Denmark)

    Shaik, Shahnoor S.; Obata, Toshihiro; Hebelstrup, Kim H

    2016-01-01

    -Phosphorylated (HP) starch chemotypes, respectively. We studied the influence of these alterations on primary metabolism, grain composition, starch structural features and starch granule morphology over caryopsis development at 10, 20 and 30 days after pollination (DAP) and at grain maturity. While HP showed...

  13. Penta-substituted benzimidazoles as potent antagonists of the calcium-sensing receptor (CaSR-antagonists).

    Science.gov (United States)

    Gerspacher, Marc; Altmann, Eva; Beerli, René; Buhl, Thomas; Endres, Ralf; Gamse, Rainer; Kameni-Tcheudji, Jacques; Kneissel, Michaela; Krawinkler, Karl Heinz; Missbach, Martin; Schmidt, Alfred; Seuwen, Klaus; Weiler, Sven; Widler, Leo

    2010-09-01

    A series of novel benzimidazole derivatives has been designed via a scaffold morphing approach based on known calcilytics chemotypes. Subsequent lead optimisation led to the discovery of penta-substituted benzimidazoles that exhibit attractive in vitro and in vivo calcium-sensing receptor (CaSR) inhibitory profiles. In addition, synthesis and structure-activity relationship data are provided.

  14. Kocuria indica sp nov., isolated from sediment sample

    Digital Repository Service at National Institute of Oceanography (India)

    Dastager, S.G.; Tang, S.K.; Krishnamurthi, S.; Lee, J.C.; Li, W.-J.

    :0, iso-C16:0, and anteiso-C17:0. The DNA G+C content of strain NIO-1021T was 60.5 mol%. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification in the genus Kocuria. Based on phenotypic, chemotypic and molecular...

  15. Population analysis of the Fusarium graminearum species complex from wheat in China show a shift to more aggressive isolates.

    Directory of Open Access Journals (Sweden)

    Hao Zhang

    Full Text Available A large number of Fusarium isolates was collected from blighted wheat spikes originating from 175 sampling sites, covering 15 provinces in China. Species and trichothecene chemotype determination by multilocus genotyping (MLGT indicated that F. graminearum s. str. with the 15-acetyl deoxynivalenol (15ADON chemotype and F. asiaticum with either the nivalenol (NIV or the 3-acetyl deoxynivalenol (3ADON chemotype were the dominant causal agents. Bayesian model-based clustering with allele data obtained with 12 variable number of tandem repeats (VNTR markers, detected three genetic clusters that also show distinct chemotypes. High levels of population genetic differentiation and low levels of effective number of migrants were observed between these three clusters. Additional genotypic analyses revealed that F. graminearum s. str. and F. asiaticum are sympatric. In addition, composition analysis of these clusters indicated a biased gene flow from 3ADON to NIV producers in F. asiaticum. In phenotypic analyses, F. asiaticum that produce 3ADON revealed significant advantages over F. asiaticum that produce NIV in pathogenicity, growth rate, fecundity, conidial length, trichothecene accumulation and resistance to benzimidazole. These results suggest that natural selection drives the spread of a more vigorous, more toxigenic pathogen population which also shows higher levels of fungicide resistance.

  16. Review of the traditional uses, phytochemistry, pharmacology and toxicology of giant fennel (Ferula communis L. subsp. communis).

    Science.gov (United States)

    Akaberi, Maryam; Iranshahy, Milad; Iranshahi, Mehrdad

    2015-11-01

    Ferula communis L., subsp. communis, namely giant fennel, has extensively been used in traditional medicine for a wide range of ailments. Fresh plant materials, crude extracts and isolated components of F. communis showed a wide spectrum of in vitro and in vivo pharmacological properties including antidiabetic, antimicrobial, antiproliferative, and cytotoxic activities. The present paper, reviews the traditional uses, botany, phytochemistry, pharmacology and toxicology of F. communis in order to reveal its therapeutic potential and future research opportunities. A bibliographic literature search was conducted in different scientific databases and search engines including Scopus, Cochrane Library, Embase, Google Scholar, Pubmed, SciFinder, and Web of science. Phytochemical studies have led to the isolation of different compounds such as sesquiterpenes from F. communis. This plant has two different chemotypes, the poisonous and non-poisonous chemotypes. Each chemotype is endowed with various constituents and different activities. The poisonous chemotype exhibits anticoagulant and cytotoxic activities with sesquiterpene coumarins as major constituents, while the non-poisonous one exhibits estrogenic and cytotoxic effects with daucane sesquiterpene esters as the main compounds. In addition, although various pharmacological properties have been reported for F. communis, anti-microbial activities of the plant have been investigated in most studies. Studies revealed that F. communis exhibits different biological activities, and contains various bioactive compounds. Although, antibacterial and cytotoxic activities are the two main pharmacological effects of this plant, further studies should focus on the mechanisms underlying these actions, as well as on those biological activities that have been reported traditionally.

  17. Gene Sequence Based Clustering Assists in Dereplication of Pseudoalteromonas luteoviolacea Strains with Identical Inhibitory Activity and Antibiotic Production

    Directory of Open Access Journals (Sweden)

    Lone Gram

    2012-08-01

    Full Text Available Some microbial species are chemically homogenous, and the same secondary metabolites are found in all strains. In contrast, we previously found that five strains of P. luteoviolacea were closely related by 16S rRNA gene sequence but produced two different antibiotic profiles. The purpose of the present study was to determine whether such bioactivity differences could be linked to genotypes allowing methods from phylogenetic analysis to aid in selection of strains for biodiscovery. Thirteen P. luteoviolacea strains divided into three chemotypes based on production of known antibiotics and four antibacterial profiles based on inhibition assays against Vibrio anguillarum and Staphylococcus aureus. To determine whether chemotype and inhibition profile are reflected by phylogenetic clustering we sequenced 16S rRNA, gyrB and recA genes. Clustering based on 16S rRNA gene sequences alone showed little correlation to chemotypes and inhibition profiles, while clustering based on concatenated 16S rRNA, gyrB, and recA gene sequences resulted in three clusters, two of which uniformly consisted of strains of identical chemotype and inhibition profile. A major time sink in natural products discovery is the effort spent rediscovering known compounds, and this study indicates that phylogeny clustering of bioactive species has the potential to be a useful dereplication tool in biodiscovery efforts.

  18. Review of the traditional uses, phytochemistry, pharmacology and toxicology of giant fennel (Ferula communis L. subsp. communis)

    Science.gov (United States)

    Akaberi, Maryam; Iranshahy, Milad; Iranshahi, Mehrdad

    2015-01-01

    Ferula communis L., subsp. communis, namely giant fennel, has extensively been used in traditional medicine for a wide range of ailments. Fresh plant materials, crude extracts and isolated components of F. communis showed a wide spectrum of in vitro and in vivo pharmacological properties including antidiabetic, antimicrobial, antiproliferative, and cytotoxic activities. The present paper, reviews the traditional uses, botany, phytochemistry, pharmacology and toxicology of F. communis in order to reveal its therapeutic potential and future research opportunities. A bibliographic literature search was conducted in different scientific databases and search engines including Scopus, Cochrane Library, Embase, Google Scholar, Pubmed, SciFinder, and Web of science. Phytochemical studies have led to the isolation of different compounds such as sesquiterpenes from F. communis. This plant has two different chemotypes, the poisonous and non-poisonous chemotypes. Each chemotype is endowed with various constituents and different activities. The poisonous chemotype exhibits anticoagulant and cytotoxic activities with sesquiterpene coumarins as major constituents, while the non-poisonous one exhibits estrogenic and cytotoxic effects with daucane sesquiterpene esters as the main compounds. In addition, although various pharmacological properties have been reported for F. communis, anti-microbial activities of the plant have been investigated in most studies. Studies revealed that F. communis exhibits different biological activities, and contains various bioactive compounds. Although, antibacterial and cytotoxic activities are the two main pharmacological effects of this plant, further studies should focus on the mechanisms underlying these actions, as well as on those biological activities that have been reported traditionally. PMID:26949491

  19. Piperazine scaffolds via isocyanide-based multicomponent reactions

    NARCIS (Netherlands)

    Dömling, Alexander; Huang, Yijun

    2010-01-01

    Piperazine scaffolds are amongst the most extensively used backbones in medicinal chemistry and many bioactive compounds are built upon this template. The physicochemical properties and the three-dimensional structures of the different piperazine chemotypes are of utmost importance to understanding

  20. Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae).

    Science.gov (United States)

    Marčetić, Mirjana; Kovačević, Nada; Lakušić, Dmitar; Lakušić, Branislava

    2017-03-01

    Plant specialised metabolites like essential oils are highly variable depending on genetic and various ecological factors. The aim of the present work was to characterise essential oils of the species Seseli rigidum Waldst. & Kit. (Apiaceae) in various organs on the individual and populational levels. Geographical variability and the impact of climate and soil type on essential oil composition were also investigated. Individually sampled essential oils of roots, aerial parts and fruits of plants from seven populations were analysed by GC-FID and GC-MS. The investigated populations showed high interpopulational and especially intrapopulational variability of essential oil composition. In regard to the variability of essential oils, different chemotypes were defined. The essential oils of S. rigidum roots represented a falcarinol chemotype, oils of aerial parts constituted an α-pinene or α-pinene/sabinene chemotype and fruit essential oils can be characterised as belonging to a complex sabinene/α-pinene/β-phellandrene/falcarinol/germacrene B chemotype. At the species level, analysis of variance (ANOVA), principal component analysis (PCA) and canonical discriminant analysis (CDA) showed that the plant part exerted the strongest influence on the composition of essential oils. Climate had a high impact on composition of the essential oils of roots, aerial parts and fruits, while influence of the substrate was less pronounced. The variations in main compounds of essential oils based on climate or substrate were complex and specific to the plant part.

  1. A Population Genetics Framework for Understanding Aggressiveness and Toxigenicity of Fusarium Head Blight Pathogens

    Science.gov (United States)

    A 14-fold increase in the frequency of 3ADON-producing F. graminearum occured between 1998 and 2004 in western Canada. Significant population structure associated with trichothecene chemotype differences was observed, and isolates from the 3ADON populations were found to accumulate significantly mo...

  2. Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

    Science.gov (United States)

    Qiu, Jianbo; Shi, Jianrong

    2014-01-01

    Members of the Fusarium graminearum species complex (FGSC) are important pathogens on wheat, maize, barley, and rice in China. Harvested grains are often contaminated by mycotoxins, such as the trichothecene nivalenol (NIV) and deoxynivalenol (DON) and the estrogenic mycotoxin zearalenone (ZEN), which is a big threat to humans and animals. In this study, 97 isolates were collected from maize, wheat, and rice in Jiangsu and Anhui provinces in 2013 and characterized by species- and chemotype-specific PCR. F. graminearum sensu stricto (s. str.) was predominant on maize, while most of the isolates collected from rice and wheat were identified as F. asiaticum. Fusarium isolates from three hosts varied in trichothecene chemotypes. The 3-acetyldeoxynivalenol (3ADON) chemotype predominated on wheat and rice population, while 15ADON was prevailing in the remaining isolates. Sequence analysis of the translation elongation factor 1α and trichodiene synthase indicated the accuracy of the above conclusion. Additionally, phylogenetic analysis suggested four groups with strong correlation with species, chemotype, and host. These isolates were also evaluated for their sensitivity to carbendazim and mycotoxins production. The maize population was less sensitive than the other two. The DON levels were similar in three populations, while those isolates on maize produced more ZEN. More DON was produced in carbendazim resistant strains than sensitive ones, but it seemed that carbendazim resistance had no effect on ZEN production in wheat culture. PMID:25093387

  3. Acaricidal activity of essential oils from Lippia alba genotypes and its major components carvone, limonene, and citral against Rhipicephalus microplus.

    Science.gov (United States)

    Peixoto, Magna Galvão; Costa-Júnior, Livio Martins; Blank, Arie Fitzgerald; Lima, Aldilene da Silva; Menezes, Thays Saynara Alves; Santos, Darlisson de Alexandria; Alves, Péricles Barreto; Cavalcanti, Sócrates Cabral de Holanda; Bacci, Leandro; Arrigoni-Blank, Maria de Fátima

    2015-05-30

    The goal of the present study was to evaluate the acaricidal potential of Lippia alba essential oil, citral chemotypes (LA-10 and LA-44 genotypes) and carvone chemotypes (LA-13 and LA-57 genotypes), as well as purified citral and enantiomers of carvone and limonene. Efficacy against Rhipicephalus microplus was assessed by the larval packet and the engorged female immersion tests. Citral chemotypes had greater larvicidal activity than carvone chemotypes, and this was further supported by larvicidal and adulticidal activity of purified citral with LC50 values of 7.0 and 29.8 mg/mL, respectively. While purified enantiomers of carvone exhibited greater larvicidal activity than those of limonene, enantioselectivity of limonene was observed with R-(+) displaying significantly higher efficacy (LC50 of 31.2mg/mL) than S-(-) (LC50 of 54.5mg/mL). The essential oils and purified compounds were much less toxic toward engorged adult females, with the exception of citral, and this may be due to limited cuticular penetration.

  4. The Red Queen race between parasitic chytrids and their host, Planktothrix: a test using a time series reconstructed from sediment DNA.

    Directory of Open Access Journals (Sweden)

    Marcia Kyle

    Full Text Available Parasitic chytrid fungi (phylum Chytridiomycota are known to infect specific phytoplankton, including the filamentous cyanobacterium Planktothrix. Subspecies, or chemotypes of Planktothrix can be identified by the presence of characteristic oligopeptides. Some of these oligopeptides can be associated with important health concerns due to their potential for toxin production. However, the relationship between chytrid parasite and Planktothrix host is not clearly understood and more research is needed. To test the parasite-host relationship over time, we used a sediment core extracted from a Norwegian lake known to contain both multiple Planktothrix chemotype hosts and their parasitic chytrid. Sediment DNA of chytrids and Planktothrix was amplified and a 35-year coexistence was found. It is important to understand how these two antagonistic species can coexistence in a lake. Reconstruction of the time series showed that between 1979-1990 at least 2 strains of Planktothrix were present and parasitic pressure exerted by chytrids was low. After this period one chemotype became dominant and yet showed continued low susceptibility to chytrid parasitism. Either environmental conditions or intrinsic characteristics of Planktothrix could have been responsible for this continued dominance. One possible explanation could be found in the shift of Planktothrix to the metalimnion, an environment that typically consists of low light and decreased temperatures. Planktothrix are capable of growth under these conditions while the chytrid parasites are constrained. Another potential explanation could be due to the differences between cellular oligopeptide variations found between Planktothrix chemotypes. These oligopeptides can function as defense systems against chytrids. Our findings suggest that chytrid driven diversity was not maintained over time, but that the combination of environmental constraints and multiple oligopeptide production to combat chytrids

  5. The Red Queen race between parasitic chytrids and their host, Planktothrix: a test using a time series reconstructed from sediment DNA.

    Science.gov (United States)

    Kyle, Marcia; Haande, Sigrid; Ostermaier, Veronika; Rohrlack, Thomas

    2015-01-01

    Parasitic chytrid fungi (phylum Chytridiomycota) are known to infect specific phytoplankton, including the filamentous cyanobacterium Planktothrix. Subspecies, or chemotypes of Planktothrix can be identified by the presence of characteristic oligopeptides. Some of these oligopeptides can be associated with important health concerns due to their potential for toxin production. However, the relationship between chytrid parasite and Planktothrix host is not clearly understood and more research is needed. To test the parasite-host relationship over time, we used a sediment core extracted from a Norwegian lake known to contain both multiple Planktothrix chemotype hosts and their parasitic chytrid. Sediment DNA of chytrids and Planktothrix was amplified and a 35-year coexistence was found. It is important to understand how these two antagonistic species can coexistence in a lake. Reconstruction of the time series showed that between 1979-1990 at least 2 strains of Planktothrix were present and parasitic pressure exerted by chytrids was low. After this period one chemotype became dominant and yet showed continued low susceptibility to chytrid parasitism. Either environmental conditions or intrinsic characteristics of Planktothrix could have been responsible for this continued dominance. One possible explanation could be found in the shift of Planktothrix to the metalimnion, an environment that typically consists of low light and decreased temperatures. Planktothrix are capable of growth under these conditions while the chytrid parasites are constrained. Another potential explanation could be due to the differences between cellular oligopeptide variations found between Planktothrix chemotypes. These oligopeptides can function as defense systems against chytrids. Our findings suggest that chytrid driven diversity was not maintained over time, but that the combination of environmental constraints and multiple oligopeptide production to combat chytrids could have allowed one

  6. Evidence for a reversible drought induced shift in the species composition of mycotoxin producing Fusarium head blight pathogens isolated from symptomatic wheat heads.

    Science.gov (United States)

    Beyer, Marco; Pogoda, Friederike; Pallez, Marine; Lazic, Joëlle; Hoffmann, Lucien; Pasquali, Matias

    2014-07-16

    Fusarium species are fungal plant pathogens producing toxic secondary metabolites such as deoxynivalenol (DON), 15-acetyl-deoxynivalenol (15AcDON) and nivalenol (NIV). In Luxembourg, the Fusarium species composition isolated from symptomatic winter wheat heads was dominated by Fusarium graminearum sensu stricto strains (genetic 15AcDON chemotype) between the years 2009 and 2012, except for 2011, when Fusarium culmorum strains (genetic NIV chemotype) dominated the pathogen complex. Previous reports indicated that F. graminearum sensu stricto (genetic 15AcDON chemotype) was also most frequently isolated from randomly sampled winter wheat kernels including symptomatic as well as asymptomatic kernels in 2007 and 2008. The annual precipitation (average of 10 weather stations scattered across the country) decreased continuously from 924.31mm in 2007 over 917.15mm in 2008, to 843.38mm in 2009, 736.24mm in 2010, and 575.09mm in 2011. In 2012, the annual precipitation increased again to 854.70mm. Hardly any precipitation was recorded around the time of wheat anthesis in the years 2010 and 2011, whereas precipitation levels >50mm within the week preceding anthesis plus the week post anthesis were observed in the other years. The shift to genetic NIV chemotype F. culmorum strains in 2011 was accompanied by a very minor elevation of average NIV contents (2.9ngg(-1)) in the grain. Our data suggest that high NIV levels in Luxembourgish winter wheat are at present rather unlikely, because the indigenous F. culmorum strains with the genetic NIV chemotype seem to be outcompeted under humid in vivo conditions by F. graminearum DON producing strains on the one hand and seem to be inhibited - even though to a lower extent than DON producing strains - under dry in vivo conditions on the other hand.

  7. Effect of environmental factors on Fusarium population and associated trichothecenes in wheat grain grown in Jiangsu province, China.

    Science.gov (United States)

    Dong, Fei; Qiu, Jianbo; Xu, Jianhong; Yu, Mingzheng; Wang, Shufang; Sun, Yue; Zhang, Gufeng; Shi, Jianrong

    2016-08-02

    The present study was performed to identify prevailing Fusarium species and the environmental factors affecting their frequencies and the contamination of grain with major mycotoxins in Jiangsu province. The precipitation levels were 184.2mm, 156.4mm, and 245.8mm in the years 2013-2015, respectively, and the temperature fluctuated by an average of 10.6±7.2°C in 2013, 10.9±7.2°C in 2014, and 10.6±6.3°C in 2015. Co-occurrence of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3ADON), and 15-acetyldeoxynivalenol (15ADON) were observed in wheat. The average concentrations of DON were 879.3±1127.8, 627.8±640.5, and 1628.6±2,168.0μg/kg in 2013-2015, respectively. The average concentrations of 3ADON were 43.5±59.0, 71.2±102.5, and 33.5±111.9μg/kg in 2013-2015, respectively. We found that the average concentration of DON in wheat was positively correlated with precipitation (r=0.998, pFusarium asiaticum is the primary pathogenic fungus prevalent in the Fusarium head blight disease nursery. The trichothecene chemotype composition differed between Fusarium graminearum sensu stricto (s. str.) and F. asiaticum isolates. The 3ADON chemotype was found only among strains of F. asiaticum. The NIV chemotype was not observed among strains of F. graminearum, while the 15ADON chemotype represented 100% of the F. graminearum strains collected. The results of this study indicated no correlations between environmental conditions and the species or genetic chemotype composition of pathogens in Jiangsu province in 2013-2015. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Repellent activity of catmint, Nepeta cataria, and iridoid nepetalactone isomers against Afro-tropical mosquitoes, ixodid ticks and red poultry mites.

    Science.gov (United States)

    Birkett, Michael A; Hassanali, Ahmed; Hoglund, Solveig; Pettersson, Jan; Pickett, John A

    2011-01-01

    The repellent activity of the essential oil of the catmint plant, Nepeta cataria (Lamiaceae), and the main iridoid compounds (4aS,7S,7aR) and (4aS,7S,7aS)-nepetalactone, was assessed against (i) major Afro-tropical pathogen vector mosquitoes, i.e. the malaria mosquito, Anopheles gambiae s.s. and the Southern house mosquito, Culex quinquefasciatus, using a World Health Organisation (WHO)-approved topical application bioassay (ii) the brown ear tick, Rhipicephalus appendiculatus, using a climbing repellency assay, and (iii) the red poultry mite, Dermanyssus gallinae, using field trapping experiments. Gas chromatography (GC) and coupled GC-mass spectrometry (GC-MS) analysis of two N. cataria chemotypes (A and B) used in the repellency assays showed that (4aS,7S,7aR) and (4aS,7S,7aS)-nepetalactone were present in different proportions, with one of the oils (from chemotype A) being dominated by the (4aS,7S,7aR) isomer (91.95% by GC), and the other oil (from chemotype B) containing the two (4aS,7S,7aR) and (4aS,7S,7aS) isomers in 16.98% and 69.83% (by GC), respectively. The sesquiterpene hydrocarbon (E)-(1R,9S)-caryophyllene was identified as the only other major component in the oils (8.05% and 13.19% by GC, respectively). Using the topical application bioassay, the oils showed high repellent activity (chemotype A RD(50)=0.081 mg cm(-2) and chemotype B RD(50)=0.091 mg cm(-2)) for An. gambiae comparable with the synthetic repellent DEET (RD(50)=0.12 mg cm(-2)), whilst for Cx. quinquefasciatus, lower repellent activity was recorded (chemotype A RD(50)=0.34 mg cm(-2) and chemotype B RD(50)=0.074 mg cm(-2)). Further repellency testing against An. gambiae using the purified (4aS,7S,7aR) and (4aS,7S,7aS)-nepetalactone isomers revealed overall lower repellent activity, compared to the chemotype A and B oils. Testing of binary mixtures of the (4aS,7S,7aR) and (4aS,7S,7aS) isomers across a range of ratios, but all at the same overall dose (0.1 mg), revealed not only a

  9. Bioactive heterocyclic alkaloids with diterpene structure isolated from traditional Chinese medicines.

    Science.gov (United States)

    Xu, Tengfei; Liu, Shu; Meng, Lulu; Pi, Zifeng; Song, Fengrui; Liu, Zhiqiang

    2016-07-15

    The diterpenoid alkaloids as one type of heterocyclic alkaloids have been found in many traditional herbal medicines, such as genus Consolida, Aconitum, and Delphinium (Ranunculaceae). Pharmacological researches have indicated that many diterpenoid alkaloids are the main bioactive components which have analgesic, anti-inflammatory, anti-microbial, anti-tumor, cardiotonic, and anti-arrhythmic activities. Studies focused on the determination, quantitation and pharmacological properties of these alkaloids have dramatically increased during the past few years. Up to now, newly discovered diterpenoid alkaloids with important biological activities have been isolated and synthesized. Considering their significant role and diffusely used in many disease treatments, we summarized the information of their analysis methods, bioactivity, metabolism and biotransformation in vivo as well as the pharmacological mechanisms. Based on above review, the further researches are suggested.

  10. A Short Synthesis of Natural Isocoumarin Glucoside Delphoside

    Institute of Scientific and Technical Information of China (English)

    SAEED,Aamer

    2005-01-01

    A simple synthesis of delphoside, 3-methyl-6-hydroxy-8-O-β-D-glucopyranosyloxy isocoumarin (1), isolated from Delphinium spp. is described. 3,5-Dimethoxyhomophthalic anhydride (2) on treatment with acetyl chloride in the presence of 1,1,3,3-tetramethylguanidine (TMG) and triethyl amine afforded the 6,8-dimethoxy-3-methylisocoumarin (3). Regioselective demethylation of the latter furnished 8-hydroxy-6-methoxy-3-methylisocoumarin (4). Glycosylation with O-(2,3,4,6-tetra-O-acetyl-D-glucopyranosyl)trichloroacetimidate in presence of catalytic amount of boron trifluoride etherate followed by deacetylation using 5% potassium carbonate afforded 3-methyl-6-methoxy-8-O-β-D-glucopyranosyloxyisocoumarin (6) that was finally demethylated to yield delphoside 1.

  11. Study on the Supramolecular Inclusion Complex of β-Cyclodextrin/Lappaconitine

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ Lappaconitine (Lap) is a diterpenoid akaliamide, naturally occurring in roots and rhizomes of Aconitum and delphinium. Lap reveals bradycardic, hypotensive, antinocieptive activity. However, its application is restrained owing to its poor water solubility, toxicity and side effects on humans. In a number of pharmaceutical studies,CDs have been reported to interact with many drug molecules to form inclusion complexes. These inclusion complexes have been extensively used to improve water solubility of poorly soluble drugs, to reduce their toxicity, and to increase the dissolution rate [1]. In the present work, the β-CD/Lap complex was prepared by kneading method. The products have been characterized by the solubility measurement as well as UV, FTIR, NMR spectroscopy and X-ray powder diffractometry.

  12. NEW ASPECTS OF THE ALPINE VEGETATION OF PARÂNG MOUNTAINS (SOUTH CARPATHIANS

    Directory of Open Access Journals (Sweden)

    SIMON TIBOR

    2012-12-01

    Full Text Available 4 plant communities unknown in the the European syntaxonomy are described from the alpine and subalpine belts of Parâng Mountains, based on vegetation studies of the authors during 1955–1960. These are: Arabis alpina-Saxifraga aizoides, Arabis alpina-Delphinium elatum, Dianthus tenuifolius-Festuca dalmatica and the Primula minima-Dryas octopetala communities. These communities could be described later as new associations in the possession of more relevés from different localities. The East and South Carpathian Doronico carpatici-Festucetum pictae association is distinguished under this new name from the Festucetum pictae Krajina described from the Tatra Mountains. 7 further associations are found as new to the Parâng Mountains.

  13. Study on the Supramolecular Inclusion Complex of

    Institute of Scientific and Technical Information of China (English)

    Liao; Kaijun

    2001-01-01

    Lappaconitine (Lap) is a diterpenoid akaliamide, naturally occurring in roots and rhizomes of Aconitum and delphinium. Lap reveals bradycardic, hypotensive, antinocieptive activity. However, its application is restrained owing to its poor water solubility, toxicity and side effects on humans. In a number of pharmaceutical studies,CDs have been reported to interact with many drug molecules to form inclusion complexes. These inclusion complexes have been extensively used to improve water solubility of poorly soluble drugs, to reduce their toxicity, and to increase the dissolution rate [1]. In the present work, the β-CD/Lap complex was prepared by kneading method. The products have been characterized by the solubility measurement as well as UV, FTIR, NMR spectroscopy and X-ray powder diffractometry.  ……

  14. 治疗疱疹病毒和肝炎病毒感染的植物药

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    本品由下述植物的干燥粉末或它们的提取物组成:苦艾Artemisia absinthium、乳香Pistacia lentiscus树脂、裸露翠雀花Delphinium denudatum果实,也可以加些蔷薇花瓣、小豆蔻果实和具苞滇紫草Onosma bracteatum花(单甩或混合物均可),以发挥协同作用。本品具有抗病毒、抑病毒和抗过敏活性,用于治疗疱疹病毒感染,

  15. I rapporti diplomatici fra il re Ugo di Provenza e il califfo 'Abd ar-Ramân III: fonti cristiane e fonti arabe a confronto

    Directory of Open Access Journals (Sweden)

    Catia Renzi Rizzo

    2002-06-01

    Full Text Available Il saggio analizza un passo della cronaca di Ibn Hayyân: la tregua concessa dal califfo Abd ar- Rahman III ad alcuni sovrani cristiani intorno al 940.  Era tra loro il re italico Ugo di Provenza, il quale ottenne garanzie per i mercanti italici e per le loro merci. Fu quello l’inizio di un commercio regolare tra mercanti italici e la corte andalusa, e i primi ad approfittare dell’opportunità furono Amalfitani e Sardi, che portarono a Cordova, per la prima volta, broccati e lingotti d’argento. Ma vi sono altri dati importanti che emergono dal confronto tra le fonti disponibili: il ruolo significativo giocato dal re Ugo, il quale, come sua madre Berta di Toscana, cercò di ampliare gli scambi, commerciali e non, nel Mediterraneo occidentale; la funzione cruciale di Barcellona e dei conti catalani quali intermediari tra mondo cristiano occidentale e al-Andalus.

  16. Nitrogen availability, leaf life span and nitrogen conservation mechanisms in leaves of tropical trees Disponibilidade de nitrogênio, longevidade foliar e mecanismos de conservação de nitrogênio em folhas de espécies arbóreas tropicais

    Directory of Open Access Journals (Sweden)

    Guilherme Nascimento Corte

    2009-12-01

    Full Text Available Evergreen species of temperate regions are dominant in low-nutrient soils. This feature is attributed to more efficient mechanisms of nutrient economy. Nevertheless, the cashew (Anacardium occidentale- Anacardiaceae, a deciduous species, is native to regions in Brazil with sandy soil, whilst the annatto (Bixa orellana- Bixaceae, classified as an evergreen species native to tropical America, grows spontaneously in regions with more humid soils. Evergreens contain robust leaves that can resist adverse conditions for longer. The physical aspects of the leaves and mechanisms of nutrient economy between the two species were compared, in order to verify whether the deciduous species had more efficient mechanisms that might explain its occurrence in regions of low soil fertility. The mechanisms of nitrogen economy were also compared for the two species at available concentrations of this nutrient. The following were analysed: (i leaf life span, (ii physical leaf characteristics (leaf mass per area, and rupture strain, (iii nitrogenous compounds (nitrogen, chlorophyll, and protein, (iv nitrogen conservation mechanisms (nitrogen resorption efficiency, resorption proficiency, and use efficiency, and (v nitrogen conservation mechanisms under different availability of this mineral. The higher values of leaf mass per area and leaf rupture strain found in A. occidentale were related to its longer leaf life span. A. occidentale showed lower concentrations of nitrogen and protein in the leaves than B. orellana. Under lower nitrogen availability, A. occidentale had higher nitrogen resorption proficiency, nitrogen use efficiency and leaf life span than B. orellana. These characteristics may contribute to the adaptation of this species to sandy soils with low nitrogen content.Perenifólias de clima temperado são dominantes em solos pouco férteis. Essa característica é atribuída a mecanismos mais eficientes de economia de nutrientes. O cajueiro (Anacardium

  17. PENGARUH EKSTRAK BEBERAPA TANAMAN OBAT TERHADAP USUS TERISOLASI

    Directory of Open Access Journals (Sweden)

    B. Dzulkarnain

    2012-09-01

    Full Text Available The extraction of Anacardium occidentale L.leaves, Aegle marmelos Corr leaves and wood bark, Acorus calamus L. tuber and Desmodium triquetrum D.C. leaves has been tested on the isolated rabbit and guinea pig intestine. The extraction of A. occidentale L. leaves stimulated the isolated rabbit and guinea pig intestine which may due to the anacardic acid content. No consistent influence was seen by the extraction of A.marmelos Corr. leaves and wood bark. The A. calamus L. tuber extraction decreases the isolated intestine activities which is of the atropine-like type not antihistamin one. This may explain the use as antidysentri agent from the motility point of view. The D. triquetrum D.C. leaves extraction stimulated the isolated intestine which has a pilocarpine and histamine-like activity but does not exclude a seretonine-like action.

  18. Michael Tenzer, ed., Analytical Studies in World Music

    OpenAIRE

    Martin,Denis-Constant

    2007-01-01

    Pour Michael Tenzer, professeur de musique à l’Université de Colombie britannique et spécialiste (analyste et interprète) de musique pour gamelan, la réunion des textes proposés dans cet ouvrage a deux objectifs : construire une théorie de la musique dans le monde qui s’émancipe des oppositions entre musiques « classiques » et « populaires », « occidentales » et « non occidentales », c’est-à-dire jeter les bases d’une théorie générale de la musique et, pour ce faire, montrer que l’analyse mus...

  19. Acupuncture

    CERN Document Server

    Chao Ming Te

    1972-01-01

    M.Bloess présente le Docteur Chao Ming Te, un des plus grands experts en médecine chinoise (acupuncture). Il a commencé ses études en Chine en médecine occidentale. Après avoir vu des très grands effets par les aiguilles, il s'est intéressé à cette médecine qui est pour nous difficile à comprendre. Il est allé à la source et a appris cette médecin en Chine et a lu les anciens textes, qui sont la base de la médecine chinoise et datent de 1000 ans avant J.C. Il a essayé de transmettre ses connaissances en médecine chinoise aux médecins occidentales et a aussi écrit un livre.

  20. Compostos voláteis em méis florais Volatile compounds in floral honeys

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Bastos De Maria

    2003-01-01

    Full Text Available A review about origin, composition and importance of volatile compounds in floral honeys is presented. Hydrocarbons, aromatic components, acids, diacids, terpenoids, ketones, aldehydes, esters and alcohols have been found in honey aroma of different botanical origin. Cis-rose oxide has been proposed as an indicator for Tilia cordata honey. Citrus honeys are known to contain methyl anthranilate, a compound which other honeys virtually lack. Linalool, phenylethylalcohol, phenylacetaldehyde, p-anisaldehyde and benzaldehyde are important contributors for the aroma of different unifloral honeys. Both isovaleric acid, gama-decalactone and benzoic acid appears to be important odourants for Anarcadium occidentale and Croton sp. honeys from Brazil. The furfurylmercaptan, benzyl alcohol, delta-octalactone, eugenol, phenylethylalcohol and guaiacol appear to be only relevant compounds for Anarcadium occidentale. The vanillin was considered an important odourant only for Croton sp..