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Sample records for del pasto guinea

  1. Aislamiento e identificación de cepas de Azospirillum sp. en pasto guinea (Panicum maximum Jacq. del Valle del Cesar Isolation and identification of Azospirillum sp. in Guinea grass (Panicum maximum Jacq. of the Valle del Cesar

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    Diana M Cárdenas

    2010-09-01

    Full Text Available Se evaluó el efecto de factores ambientales del Valle del Cesar y el manejo agronómico del pasto guinea (Panicum maximum Jacq., sobre la población bacteriana del género Azospirillum en medios de cultivo semisólidos NFb y LGI, para lo cual se utilizó un diseño experimental en parcelas divididas con un arreglo factorial de 2 (épocas climáticas: lluvia y sequía x 2 (manejos agronómicos: agroecólogico y extractivo x 3 (muestras analizadas: suelo rizosférico, raíces y hojas. Los resultados no revelaron diferencias estadísticas significativas, lo que indica que esta bacteria puede mantener su población en condiciones de estrés por diferentes mecanismos fisiológicos. A partir de estas muestras se obtuvieron 16 aislamientos pertenecientes al género Azospirillum, a los cuales se les evaluó su actividad de reducción de acetileno como indicador de la fijación biológica de nitrógeno y su capacidad en la producción de compuestos indólicos como promotores del crecimiento vegetal. Se seleccionaron las cepas SRGM2, SRGM3 y SRGM4 obtenidas de muestras de suelo rizosférico de pasto guinea de la Estación Experimental Motilonia de Corpoica, municipio Agustín Codazzi, departamento del Cesar. Estos aislamientos se caracterizaron molecularmente por el gen 16S rRNA y según el análisis BLAST en la base de datos del GenBank y presentaron 93% de similitud con A. lipoferum (SRGM2 y SRGM3 y 94% con A. brasilense (SRGM4.The effect of environmental factors of the Valle del Cesar and the agronomic management of Guinea grass (Panicum maximum Jacq., on the bacterial population of the Azospirillum genus in semisolid NFb and LGI culture media was evaluated, for which an experimental design was used in divided plots with a 2 (climatic seasons: rainy and dry x 2 (agronomic managements: agroecological and extractive x 3 (analyzed samples: rhizospheric soil, roots and leaves factorial arrangement. The results did not reveal significant statistical

  2. Factores asociados al riesgo nutricional del adulto mayor no institucionalizado, Pasto, Colombia

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    Hernández Rangel, Juanita del Pilar

    2017-01-01

    Objetivo: determinar los factores sociales, demográficos, y de salud que se asocian al riesgo nutricional de los adultos mayores no institucionalizados de la ciudad de Pasto en el año 2016. Metodología: estudio de prevalencia analítico a partir del análisis secundario de los datos para la ciudad de Pasto de la encuesta “Índice de vulnerabilidad de los adultos mayores, en tres ciudades de Colombia, 2016”. Se utilizó la Iniciativa del Tamizaje Nutricional (NSI) con la escala DETERMINE para l...

  3. Micrositios del pasto navajita (Bouteloua gracilis en comunidades de pastizal y de matorral del Altiplano Mexicano

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    Rosalva García-Sánchez

    2005-01-01

    Full Text Available El objetivo de este trabajo fue determinar si hay un tipo de micrositio específico para el establecimiento de Bouteloua gracilis, con patrones recurrentes, en dos diferentes tipos de comunidades vegetales. El pasto navajita azul es una especie perenne de alto valor forrajero y resistente a la sequía. Para ello se realizó un recorrido por tres matorrales y tres pastizales del Altiplano Mexicano; los sitios estudiados fueron elegidos con base en los reportes de ejemplares colectados y herborizados. Los resultados muestran, por un lado, que en los pastizales se presentan micrositios más de tipo funcional que físico, que determinan el establecimiento de B. gracilis, debido principalmente a los altos requerimientos de humedad edáfica de las semillas para germinar y desarrollarse. El micrositio más frecuente que determina el reclutamiento de los individuos provenientes de semilla es el suelo cercano a individuos adultos de su propia especie. Por otro lado, en los matorrales se detectaron, de manera recurrente, varias condiciones de micrositio para la especie y se observó que el tipo de micrositio es determinante, tanto para el establecimiento como para el crecimiento de este y otros pastos; también, el estudio mostró que en comunidades de matorral existe una mayor diversidad de micrositios que en los pastizales, por lo que se concluye que los individuos de B. gracilis, en los matorrales, crecen asociados a micrositios específicos que le brindan protección contra la desecación.

  4. Procesos institucionales en el pago por servicios ambientales, cuenca alta del río Pasto

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    Yesenia Alejandra Narváez

    2016-06-01

    Full Text Available El pago por servicios ambientales (PSA, es la disponibilidad que tiene un individuo o grupo social para aprovechar o conservar la oferta ambiental natural. Este estudio se realizó en cuenca alta del río Pasto, donde el principal servicio ambiental, es la provisión hídrica del 85% de los habitantes de Pasto, Nariño, convirtiéndolo en un ecosistema estratégico que requiere de alternativas viables para su conservación y manejo. El objetivo fue evaluar los procesos relacionados con PSA, adelantados por algunas Instituciones; para ello, se diseñó una entrevista estructurada, aplicada a funcionarios de cuatro instituciones, que contemplaba las variables: institución, conocimiento normativo, recursos financieros, apoyo técnico, actividades realizadas actualmente y posibles alternativas para PSA. Con el programa Decisión Explorer 3.3 y con integrantes de las instituciones se propusieron alternativas para mejorar el PSA. Se pudo determinar que en el corregimiento de Cabrera se están ejecutando las alternativas: implementación de sistemas agroforestales, familias guardabosques y adquisición de predios para avanzar en PSA. Se concluyó que en la Cuenca Alta del río Pasto, el PSA aún es incipiente pero promisorio, hay poco conocimiento institucional y los procesos se han centrado en la compra de tierras para la conservación de fuentes abastecedoras de acueductos rurales y de cabeceras municipales.

  5. Restricción del riego en la producción de biomasa del pasto Pennisetum sp

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    Jesús Enrique Chavarría Párraga

    2017-12-01

    Full Text Available La investigación se desarrolló en el sitio Garrapata, Parroquia Santa Rita, provincia Manabí; durante la época seca del año 2015. El estudio consistió en evaluar la restricción hídrica en la producción del pasto king grass morado (Pennisetum sp bajo riego por aspersión. Se utilizó un diseño de bloques completos al azar, formado de tres tratamientos y seis repeticiones. Los tratamientos consistieron en aplicar tres láminas de reposición de agua 80% ETc, 100% ETc, 120% ETc, calculados a partir de los registros diarios de evaporación en un tanque evaporímetro tipo A y afectados por el coeficientes Kp (Coeficiente de tina, método FAO 33,1980. Las variables evaluadas fueron altura de planta a los 15 y 30 días del corte de igualación del pasto, altura de planta a la cosecha (m, rendimiento de forraje verde por parcela y hectárea (kg y materia seca (%. Los resultados indican que el tratamiento lámina de riego al 120% de la ETc (T3, respondió de manera favorable en altura de planta a la cosecha (2.08 m, rendimiento (53000 kg.ha-1; el tratamiento lámina de riego al 100% de la ETc (T2 obtuvo mayor contenido de materia seca (19.86%. El pasto king grass morado regado con una lámina de riego al 120% de la ETc, constituye una modalidad que mejora el rendimiento productivo y rentable del cultivo y podría aplicarse en la zona.

  6. RESPUESTA DEL PASTO ALPISTE (Phalaris arundinacea L. A LA FERTILIZACIÓN NITROGENADA EN COSTA RICA

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    Luis Villalobos

    2016-01-01

    Full Text Available El nitrógeno es el nutriente de mayor importancia en la producción de pasturas y las dosis de fertilización utilizadas deben optimizar la producción de biomasa de alto valor nutricio - nal con un bajo costo y mínima afectación al medio ambiente. Se realizó un estudio con pasto alpiste (P halaris arundinacea L. cosechado cada 70 días en una finca comercial productora de leche ubicada en el distrito de Santa Rosa de Oreamuno, en la provincia de Cartago entre noviembre de 2010 a enero de 2012. Se aplicó 3 dosis de fertilización nitrogenada (100, 200 y 300 kg.ha -1 y se evaluó su respuesta produc - tiva en biomasa y valor nutricional por medio de un diseño de bloques completos al azar. La producción de materia seca no fue estadística - mente diferente (p≥0,05 entre las dosis menor y mayor (2956 y 3347 kg.ha -1 , respectivamente, mientras que mostró valores menores y mayores (p≤0,0001 entre los muestreos de febrero y abril (1632 y 4323 kg.ha -1 , respectivamente. Los ran - gos de concentraciones de proteína cruda (14,62- 19,20%, fibra detergente neutro (54,50-70,70% y energía neta de lactancia (1,14-1,32 Mcal.kg -1 MS fueron el resultado de los cambios significa - tivos (p≤0,0001 que se presentaron a lo largo del año en el alpiste forrajero. Estrategias de ajustes en carga animal y suplementación del ganado lechero deben considerarse para llenar las limitantes estacionales encontradas en la biomasa y el valor nutricional del pasto alpiste durante el año.

  7. Comportamiento productivo del pasto ovillo (Dactylis glomerata L. en respuesta al pastoreo.

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    Filogonio Jesús Hernández-Guzmán

    2015-01-01

    Full Text Available El objetivo del estudio fue determinar los patrones de variación en los componentes de la productividad forrajera del pasto ovillo (Dactylis glomerata L. bajo pastoreo. En Montecillo, Texcoco, Estado de México, México, de junio 2005 a junio 2006, se estudió la combinación de tres frecuencias (21, 28 y 35 días y dos intensidades (5-7 y 9-11 cm en el forraje residual de pastoreo, en un diseño de bloques al azar con tres repeticiones. Se evaluó el rendimiento anual y la distribución estacional de forraje, tasa de crecimiento, composición botánica y morfológica y densidad de tallos. La frecuencia de pastoreo de 35 días superó a las de 21 y 28 (P<0,05 en el rendimiento anual, durante primavera y verano. Independientemente de la frecuencia e intensidad de pastoreo, el rendimiento estacional se distribuyó de la siguiente forma: 49,5, 31,6, 14,1 y 4,8% para primavera, verano, invierno y otoño, respectivamente. La mayor tasa de crecimiento se registró en primavera al pastorear cada 35 días y la menor en otoño al pastorear cada 28 días (P<0,01. Durante invierno se dio mayor presencia de otras especies, siendo el pastoreo cada veintiún días a una intensidad ligera el que originó un aporte al rendimiento de otras especies del 60%. No se presentaron efectos de intensidad ni de frecuencia de pastoreo en la densidad de tallos. El mayor rendimiento y tasa de crecimiento se obtuvieron al pastorear cada 35 días.

  8. Caracterización de fincas adoptadoras del pasto Andropogon gayanus en el departamento del Cesar, Colombia Caracterización de fincas adoptadoras del pasto Andropogon gayanus en el departamento del Cesar, Colombia

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    Sere Carlos

    1987-09-01

    Full Text Available La razón expuesta por ganaderos para adoptar el pasto A. gayanus es la de poseer una especie que les provea forraje en la época seca. Quienes mayor éxito han obtenido, hasta el momento, son los adoptadores de suelos arenosos o de sabana en la parte central del departamento. En dicha zona se presentan factores de suelo como acidez, existencia de aluminio, texturas arenosas y buenos drenajes; la temperatura promedio es de 26°C, precipitaciones anuales entre 1200 y 2500 mm con una época seca (150 días sumamente fuerte. El comportamiento del pasto está limitado en la parte sur del departamento por exceso de humedad y en el norte por la presencia de bases y pH elevados. Desde el punto de vista económico, el éxito de la introducción de A. gayanus está relacionado con factores como el bajo costo de oportunidad de las tierras, la baja inversión adicional en ganados, los bajos costos de establecimiento, la complementaridad con otras pasturas en tierras mejores en la misma finca y la elevada rentabilidad marginal de la tecnología que posibilita la adopción aún en años sin incentivos para invertir en ganadería.This study was carried out in the Cesar Department, Colombia, to identify factors that have led to the adoption of A. gayanus. The expressed reason of r a n e hers is the need for a species that will supply forage during the dry season. Adopters on sandy soils in the middle part of the Zone are up to now those that most succes have been. Soils in this region are characterized by its acidity, presence of aluminum, sandy textures and good drainage. Mean annual temperatures are of 26°C with annual rainfall ranging between 1200 and 2500 mm with a marked dry season (150 days. In the south of the department rainfall is excessive while in the north high base saturation and pH of the soils do not allow the grass success fully competence. Economically, the success of promoting A. gayanus is in relation to some factors such as low opportunity

  9. EFECTO DEL PICADO SOBRE LAS CARACTERÍSTICAS NUTRICIONALES Y FERMENTATIVAS DE ENSILAJES DE PASTOS KIKUYO, RYEGRASS PERENNE Y ALPISTE FORRAJERO

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    Luis Villalobos

    2016-01-01

    Full Text Available Se evaluó el efecto del picado sobre las características nutricionales y fermentativas de ensilajes elaborados con 3 pastos (kikuyo, rye-grass perenne y alpiste forrajero. Se elaboraron 2 microsilos de cada pasto en bolsas de polieti-leno con capacidad para 1 kg, aplicando 2 trata-mientos (picado y sin picar; se adicionó a cada microsilo 3% de melaza y 0,1% de un inóculo artesanal (hecho de leche agria, suero de queso y melaza. La especie de pasto tuvo el mayor impacto sobre las características nutricionales y fermentativas de los microsilos, mientras que el picado no mostró tendencia clara sobre los parámetros evaluados. El contenido de PC (14,40 a 19,35%, FDN (46,25 a 63,16% y la DIVMS (46,15 a 65,55% en los ensilados es indicador de su potencial para llenar gran parte de los requerimientos nutricionales del ganado lechero. El pasto ryegrass ensilado alcanzó los niveles de pH (4,41 a 4,83 menores y el pasto kikuyo los valores menores de capacidad amor-tiguadora (79,12 a 81,17 mEq NaOH 100 g-1 MS y de nitrógeno amoniacal (1,03 a 1,33%. Los ensilajes elaborados con pastos pueden uti-lizarse como suplementos con valor nutricional superior a los henos utilizados en las raciones de ganado lechero en zonas altas.

  10. RESPUESTA DEL PASTO PALMERA (Setaria poiretiana AL SOMBRIO: UNA OPCION DE PRODUCCIÓN EN SISTEMAS SILVOPASTORILES

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    NELSON JOSÉ VIVAS-QUILA

    2014-06-01

    Full Text Available La investigación se llevó a cabo en la granja Los Naranjos ubicada en la Vereda La Venta del Municipio de Cajibío del Departamento del Cauca. La sombra de los árboles no permite el progreso del forraje cultivado en su entorno y no se conocen especies tolerantes a la sombra que se adapten a las condiciones de clima medio y suelos de mediana a baja fertilidad.Teniendo en cuenta lo anterior se adelantó el estudio sobre el manejo agronómico y potencial forrajero del pasto palmera (Setaria poiretiana bajo tres ambientes diferentes de sombra. La evaluación se desarrolló bajo el diseño en bloques completos al azar con 3 tratamientos y 4 repeticiones. Los tratamientos en diferentes porcentajes de sombríoartificial utilizando polisombra (libre exposición, semisombra y sombra con 0,44 y 70% de sombríoy las repeticiones (bloques fueron las condiciones de sombra dadas por el sombrío del bosque en torno al proyecto. El tratamiento que mejor comportamiento agronómico presentó en cuanto a producción de materia seca fue el T1 (libre exposición; no se encontraron diferencias estadísticas para las variables vigor, cobertura, altura, porcentaje de materia seca

  11. Acid Fostatase Activity in soils croping with potato and pastures from the Catambuco, Pasto, Colombia Actividad de fosfatasa ácida en suelos cultivados con papa y praderas del corregimiento de Catambuco, Pasto-Colombia

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    Burbano Hernán

    2007-03-01

    Full Text Available To estimated the acid phosphatase activity (APA in soil covered with potato and prairie in Catambuco, place Pasto, Colombia; located to 2820 msnm and with average temperature of 12.4oC. The acid phosphatase activity obtained the biggest values in soil covered with prairie in winter time, in addition to that, there were high correlations between the APA and the organic matter off the soil. In the factors sampling times and use of the soil, there were significance statistical differences, with the most significance in soil covered with prairie and with recent use in potato cultivation. The APA obtained the biggest percentage in soil covered with prairie, in front of the soil cultivated with potato for the two evaluated times. Key words: Acid phosphatase activity, use of land, Solanum tuberosumCon el objetivo de estimar y comparar la actividad de la fostatasa ácida (AFA en suelos sembrados con papa y con pastos, se realizó la presente investigación en suelos del corregimiento de Catambuco, municipio de Pasto, Colombia (2.820 msnm, 12.4oC. La mayor actividad de la fosfatasa ácida ocurrió en pradera en época de invierno; además se encontraron altas correlaciones entre AFA y el contenido de materia orgánica. Para los factores épocas de muestreo y uso del suelo hubo diferencias estadísticas altamente significativas y para suelos cubiertos con pradera y con uso reciente en el cultivo de papa. La AFA alcanzó el mayor porcentaje en suelos cubiertos con pradera, frente a los suelos cultivados con papa, para las dos épocas evaluadas. Palabras claves: Actividad fosfatasa ácida, uso del terreno, Solanum tuberosum

  12. Situación del mercado laboral de los profesionales en San Juan de Pasto, 2010

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    Julio César Riascos

    2011-01-01

    Full Text Available En el documento se analiza la situación laboral de los profesionales de la ciudad de Pasto. Se estudian las características generales de un profesional tomado al azar y se mide la tasa de desempleo, la tasa de ocupación, la tasa general de participación y la tasa de subempleo. Asimismo se identifican los retornos en educación por área de conocimiento y se determinan los principales factores que explican los niveles de ingreso y la probabilidad de empleo formal y estable.

  13. Análisis de crecimiento del pasto maralfalfa (Pennisetum sp. en clima cálido subhúmedo

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    Jesús Miguel Calzada-Marín

    2014-01-01

    Full Text Available El objetivo de este experimento fue realizar un análisis de crecimiento del pasto “Maralfalfa” ( Pennisetum sp. desde la siembra hasta determinar el momento óptimo de cosecha. La morfología de la planta (MP, tas a de crecimiento (TC, altura, radiación interceptada (RI, relación hoja/tallo (H:T, hoja/no hoja (H:NH y la acumulación de biomasa aérea, se evaluaron a intervalos de 15 días, en un periodo de 180 días, a excepción de lo s dos primeros muestreos que fueron mensuales. Los datos se analizaron mediante el procedimiento GLM de SA S, bajo un diseño experimental de bloques al azar, con medidas repetidas en el tiempo, con tres repeticiones . La MP varió de manera significativa ( P <0.01 a través de los diferentes estados fisiológicos. La máxima producción de biomasa aérea y TC se alcanzó a los 151 días después de la siemb ra con 37,297 kg MS ha -1 y 247 kg MS ha -1 d -1 , respectivamente. También se registró el mayor porcentaje de RI (97.4 %, con una altura de 2.3 m aproximadamente. La producción de biomasa de tallos y la producción de material muerto del pasto mar alfalfa, están correlacionadas de manera positiva con el incremento en la edad de la planta. La máxima tasa d e crecimiento, coincide con la máxima producción de hojas y de tallos, y con la mayor producción de bi omasa total.

  14. RESPUESTA DEL PASTO RAIGRASS AUBADE (Lolium sp. A DOSIS DE SILICIO EN INTERACCION CON DIFERENTES DOSIS DE NPK

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    DIANA LEGARDA - LOPEZ

    Full Text Available El presente trabajo se realizó en el corregimiento de Catambuco, municipio de Pasto, localizado a 01°06'33 N y 77°19 '07.8 WO; con el objeto de conocer El presente trabajo se realizó en el corregimiento de Catambuco, municipio de Pasto, localizado a 01°06 '33 N y 77°19´07.8 WO; con el objeto de conocer con tres repeticiones y 10 tratamientos distribuidos en dosis altas, medias y bajas de NPK (Altas: 529 kg/ha de úrea, 72 kg/ha de SFT, 160 kg/ha de KCl. Medias: 397 kg/ha de urea, 54 kg/ha de SFT, 120 kg/ha de KCl. Bajas: 265 kg/ha de úrea, 36 kg/ha de SFT, 80 kg/ha de KCl contrastadas con dosis altas, medias y bajas de Si (Alta 100 kg/ha, media 75 kg/ha, baja 50 kg/ha de SiO2 y un testigo sin fertilización con Si. El Si en dosis alta y NPK alto, presentó mejores resultados en altura de planta (101cm, producción de forraje verde (167,2 Ton/ha/año, materia seca (27,44 Ton/ha/año, concentraciones de P (parte aérea 0,53%y raíz 0,42%,de K (parte aérea 3,11% y raíz 2,27%. Al aplicar dosis altas y medias en el suelo,la concentración de P y K disminuyó. El tratamiento dosis alta de NPK y alta de Si presentó el mayor beneficio económico, seguido del tratamiento dosis media de NPK y alta de Si.

  15. Evaluación agronómica y nutricional del pasto estrella africana (cynodon nlemfuensis En la zona de monte verde, puntarenas, Costa Rica. II. valor nutricional

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    Luis Villalobos

    2014-01-01

    Full Text Available Se analizó el valor nutricional del pasto estrella africana ( Cynodon nlemfuensis a lo largo de 2 años en muestreos bimensuales, en 4 fincas comerciales de ganado lechero ubicadas en los cantones de Tilarán y Central (latitud 10°20’ N, longitud 84°50’, altitud 800 a 1200 msnm de las provincias de Guanacaste y Puntarenas, respectivamente. Las muestras se recolectaron en el aparto siguiente a ser pastoreado y se utilizó una altura de cosecha de 10 cm, para simular el pastoreo que hacen los animales. La composición nutricional promedio para los 2 años de evalua - ción fue de 23,57% MS, 20,27% PC, 2,67% EE, 10,97% cenizas, 64,21% FDN, 34,95% FDA, 4,06% lignina y 68,02% DIVMS y su contenido energético para las variables de TND, ED, EM, EN L (3X y EN G fue 61,37%; 2,71; 2,05; 1,25 y 0,78 Mcal.kg -1 de MS, respectivamente. El valor nutricional del pasto estrella africana varió a lo largo del año como resultado de la climatología de la zona de Monteverde, siendo las fincas con influencia de la vertiente del Pacífico las de menor afectación en la calidad del forraje. El pasto estrella africana mostró un contenido de PC superior a lo reportado para dicha especie y, en general, para pastos tropicales, por lo cual no es limitante para la producción láctea, y la suplementación del ganado lechero en la zona debe utilizar fuentes que permitan una utiliza - ción eficiente del N soluble a nivel ruminal. La rotación del pasto estrella cada 25 días debe ser flexible para permitir, en conjunto con programas de fertilización, optimizar la productividad de las pasturas y su persistencia.

  16. El papel del microcrédito en la actividad formal empresarial en el casco urbano del municipio de Pasto 2010

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    Nicolás Madroñero Madroñero

    2011-07-01

    Full Text Available Este artículo presenta la participación del sector microempresarial total del sector empresarial formal y su aporte a la generación de empleo en San Juan de Pasto, importancia que se consagra en la ley Mipyme 590. Se hace referencia sobre los principales oferentes del microcrédito, utilizados por los microempresarios formales para financiar su actividad empresarial en la ciudad, argumentando su afinidad con base a las tasas de interés ofrecidas por cada uno de los prestamistas y, se hace un análisis de la composición de la cartera bruta en relación al crédito de consumo, hipotecario, comercial y microcrédito. De igual manera se determina la incidencia del microcrédito sobre el sector microempresarial, visto desde el punto de vista del crecimiento en el valor de activos y número de empleados; además se corrobora la fuerte relación o asociación entre este tipo de cartera y el PIB municipal. El estudio también asumió la elaboración de un punto de inflexión para determinar la trascendencia del fenómeno inflacionario en el crecimiento de la cartera microcrediticia.

  17. Rendimiento y calidad de forraje del pasto ovillo (Dactylis glomerata L. al variar la frecuencia e intensidad de pastoreo

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    Jorge Armando Villareal González

    2014-01-01

    Full Text Available El objetivo fue determinar el rendimiento y calidad del pasto ovillo ( Dactylis glomerata L., al variar la frecuencia e intensidad de pastoreo. Se evaluaron tres frecuencias (2, 3 y 4 semanas en primavera y verano y 4, 5 y 6 semanas durante otoño y dos intensidades de pastoreo (severa: 3 a 5 cm y ligera: 6 a 8 cm de altura de forr aje residual. Se evaluó el rendimiento de forraje, tasa de acumulación neta de forraje (TANF, composición morfoló gica y calidad del forraje. El mayor rendimiento acumulado y TANF se presentaron con pastoreo severo cada 4 y 6 sem anas y en ambas intensidades cada 3 y 5 semanas. El mayor rendimiento estacional (7,844; 7,699; 7114 kg MS ha -1 se presentó en verano en las mismas frecuencias e intensidades de pastoreo, respectivamente ( P <0.05. Durante primavera no se observaron dife rencias en rendimiento y TANF entre frecuencias de pastoreo. En otoño la frecuencia de 5 semanas superó a la de 4 ( P <0.05, pero no diferente a la de 6 semanas. En todas las estaciones del año la intensidad de pastoreo severo superó a la ligera, siendo diferentes en primavera y verano ( P <0.05. En general, el contenido de proteína total y digestibilidad in vitro de la materia seca tendieron a disminuir conforme aumentó el intervalo de pastoreo, siendo en promedio de 20 y 65 %, respectivamente. Se concluye que para obtener el mayor re ndimiento y calidad de forraje, los pastoreos deben realizarse cada 4 semanas durante primavera-verano y cada 5 semanas en otoño, a una intensidad severa.

  18. Rendimiento y calidad de semilla de pasto guinea (Panicum maximum Jacq.) cv. Tanzania usando la fitohormona esteroidal cidef-4

    OpenAIRE

    Bertín Maurilio Joaquín Torres; Miguel Angel Moreno Carrillo; Santiago Joaquín Cancino; Alfonso Hernández Garay; Jorge Pérez Pérez; Armando Gómez Vázquez

    2010-01-01

    El objetivo fue evaluar el efecto de la fitohormona esteroidal cidef-4 en el rendimiento y calidad de semilla en Panicum maximum cv. Tanzania. El experimento se realizó durante el 2001 en Tejupilco, Estado de México. Los tratamientos evaluados fueron, T1=testigo, T2=4 mg L-1 de ingrediente activo (i.a.) aplicados al inicio de antesis, T3=4 mg L-1 de i.a. aplicados antes del espigamiento e inicio de antesis, T4=8 mg L-1 de i.a. aplicados al inicio de antesis, T5=8 mg L-1 de i.a. aplicados ante...

  19. Rendimiento y calidad de semilla de pasto guinea (Panicum maximum Jacq.) cv. Tanzania usando la fitohormona esteroidal cidef-4

    OpenAIRE

    Joaquín Torres, Bertin Maurilio; Moreno Carrillo, Miguel Angel; Joaquín Cancino, Santiago; Hernández Garay, Alfonso; Pérez Pérez, Jorge; Gómez Vázquez, Armando

    2010-01-01

    El objetivo fue evaluar el efecto de la fitohormona esteroidal cidef-4 en el rendimiento y calidad de semilla en Panicum maximum cv. Tanzania. El experimento se realizó durante el 2001 en Tejupilco, Estado de México. Los tratamientos evaluados fueron: T1=testigo; T2=4 mg L-1 de ingrediente activo (i.a.) aplicados al inicio de antesis; T3=4 mg L-1 de i.a. aplicados antes del espigamiento e inicio de antesis; T4=8 mg L-1 de i.a. aplicados al inicio de antesis; T5=8 mg L-1 de i.a. aplicados ante...

  20. LA DINÁMICA DEL USO DE LA TIERRA EN MATO GROSSO: ASPECTOS ECONÓMICOS DEL CULTIVO DE ARROZ, SOYA Y PASTOS

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    Dilamar Dallemole

    2014-08-01

    Full Text Available El cultivo de arroz siempre estuvo presente en el rol de culturas practicadas en el cerrado brasileño, en especial, en el Mato-grossense. Dado a esa particularidad, éste estudio busca comprender mejor las relaciones existentes entre la producción de arroz con las áreas plantadas de soya, y, propiamente, con la área plantada de arroz en Mato Grosso, en el período de 1979 a 2007. Debido a la gran extensión territorial del estado, el cultivo de arroz fue siendo realizado a lo largo de los años como frontera agrícola, para apertura de nuevas áreas, con variedades adecuadas a tierras, inicialmente, con baja fertilidad. Con el desarrollo tecnológico y la posibilidad de nuevas actividades productivas, esa realidad viene se alterando y la área plantada de arroz disminuyó a lo largo de los años, perdiendo espacio para la soya y pastos. Como instrumento metodológico, se empleó un modelo de regresión múltiple, estimada por el Método de Mínimos Cuadrados Ordinarios (MQO, en que los resultados apuntan las correlaciones existentes entre las categorías mencionadas, lo que permite analizar con más propiedad lo que está ocurriendo con el segmento. Las principales conclusiones obtenidas confirman la influencia positiva de la producción de arroz con relación a suya área plantada, sin embargo, negativa para las áreas plantadas de soya, pastos y arroz en Mato Grosso. Aunque venga perdiendo espacio para soya y pastos, el cultivo de arroz, mismo con menor productividad y calidad inferior del grano, aún es practicado debido a su capacidad de adaptación en nuevas áreas, o mismo, en áreas degradadas

  1. Biodiversidad íctica de praderas de pasto marino de la costa noroeste del Golfo de Cariaco, Venezuela

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    Luis Alejandro Ariza A

    2012-06-01

    Full Text Available Los pastos marinos son ecosistemas costeros de alta productividad, con gran diversidad y abundancia de peces, la cual es aprovechada por pescadores artesanales. En este estudio se analizó la estructura comunitaria íctica de praderas de Thalassia testudinum en Manzanillo (M y La Brea (LB, costa noroeste del Golfo de Cariaco, Venezuela. También, se realizaron muestreos mensuales (11-2006 al 10-2007, con la utilización de una red playera. Se capturaron 34 810 organismos agrupados en 13 órdenes, 36 familias y 83 especies. En ambas zonas, el número de especies fue similar, aunque el número de organismos vario, y se encontró para M un total de 55 especies y 13 210 organismos y para LB 58 especies y 21 600 organismos. Las especies más abundantes y de mayor biomasa en el área muestreada fueron: Nicholsina usta, Haemulon boschmae, H. steindachneri, Harengula jaguana, Halichoeres bivittatus y Hemiramphus brasiliensis. Los visitantes ocasionales fue el componente comunitario más frecuente con 59%, los cíclicos y los residentes permanentes obtuvieron 22 y 19%, respectivamente. En M la H’n fue de 1.71±0.64bits/ind; entretanto en LB fue 1.95±0.51bits/ind. Los valores de la diversidad estuvieron relacionados directamente con la equitabilidad e inversamente con la dominancia. Los bajos valores del índice de similaridad, entre localidades permite establecer que estas comunidades ícticas son disimiles, debido quizás a la estructuración de cada pradera de Thalassia y a la conectividad con otros sistemas.

  2. Rendimiento y calidad de semilla de pasto guinea (Panicum maximum Jacq. cv. Tanzania usando la fitohormona esteroidal cidef-4

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    Bertín Maurilio Joaquín Torres

    2010-01-01

    Full Text Available El objetivo fue evaluar el efecto de la fitohormona esteroidal cidef-4 en el rendimiento y calidad de semilla en Panicum maximum cv. Tanzania. El experimento se realizó durante el 2001 en Tejupilco, Estado de México. Los tratamientos evaluados fueron, T1=testigo, T2=4 mg L-1 de ingrediente activo (i.a. aplicados al inicio de antesis, T3=4 mg L-1 de i.a. aplicados antes del espigamiento e inicio de antesis, T4=8 mg L-1 de i.a. aplicados al inicio de antesis, T5=8 mg L-1 de i.a. aplicados antes del espigamiento e inicio de antesis y T6=12 mg L-1 de i.a. aplicados únicamente al inicio de antesis. Se utilizó un diseño de bloques al azar, con tres repeticiones. Se evaluó el rendimiento de semilla total (RST, rendimiento de semilla pura (RSP, número de panículas m-2, longitud de panícula, semillas producidas y cosechadas por panícula, peso de 1,000 semillas y germinación. Se detectaron diferencias (P0.05 entre tratamientos. Se concluye que la fitohormona esteroidal cidef-4 incrementó el rendimiento de semilla de P. maximum cv. tanzania, independientemente de la concentración y momento de aplicación. La calidad de la semilla, no fue afectada por ninguno de los tratamientos evaluados.

  3. Biofiltro con cascarilla de arroz y pasto vetiver (C. Zizanioides para el tratamiento del efluente de la PTAR del INPEC – Yopal, Casanare, Colombia

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    Sandy Katheryne Higuera Infante

    2017-06-01

    Full Text Available En la investigación se evaluó un biofiltro a escala laboratorio para el tratamiento del efluente de la PTAR del Inpec de Yopal, Casanare, cuya finalidad era lograr un efluente de calidad óptima para reúso del agua en riego agrícola. Se contemplaron tres fases, la primera caracterización y análisis de los parámetros microbiológicos como fisicoquímicos del efluente. La segunda fase diseño y construcción del sistema a escala laboratorio del humedal artificial, se construyó en vidrio, usando cascarilla de arroz, intercalada con grava, se sembró pasto vetiver (C. Zizanioides y se inundó el sistema para que funcionará con flujo subsuperficial, el tiempo de retención hidráulico TRH fue de 3.4 días. La desinfección con cloro se diseñó a partir de una prueba de demanda de cloro. La tercera fase consistió en la operación del sistema a flujo continuo, el seguimiento se realizó a través de 4 muestreos con frecuencia semanal, en dos de estos muestreos se incluyó medición de DBO5, coliformes totales y fecales y conductividad.  Los resultados mostraron que la cascarilla de arroz como sustrato funciona bien, las plantas crecieron permanentemente, ayudando a disminuir la carga orgánica. Como conclusión la cascarilla puede ser un sustituto total o parcial de otros sustratos de biofiltros o humedales de flujo subsuperficial, al ser menos costosa y estar disponible especialmente en la región de la Orinoquía. Sin embargo, su uso debe complementarse con un proceso de reoxigenación del agua, ya que su condición anaerobia es un aspecto negativo para los cuerpos de agua receptores de vertimientos.

  4. CARACTERÍSTICAS MORFOGÉNICAS Y SU INFLUENCIA EN EL RENDIMIENTO DEL PASTO MOMBAZA, COSECHADO A DIFERENTES INTERVALOS DE CORTE

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    Omar Ramírez Reynoso

    2009-11-01

    Full Text Available El objetivo del estudio fue determinar el flujo de tejidos y rendimiento por corte del pasto Mombaza (Panicum maximum Jacq., cosechado a tres intervalos de corte (IC: 3, 5 y 7 semanas, durante un año, en las épocas de sequía (10-nov-2006 a 09-jun-2007 y lluvias (10-jun a 06-nov-2007. Los tratamientos (IC se distribuyeron en 12 parcelas de 17.5 m2, mediante un diseño de bloques completos al azar, con cuatro repeticiones. El IC de 3 semanas promedió mayor tasa de aparición de hojas (0.185 hojas tallo-1 d-1 durante el estudio (P

  5. Determinantes de la utilización de los servicios de salud bucal en estudiantes universitarios del municipio de Pasto, 2011 / Determinants of dental health service use among university students in the Pasto municipality, 2011

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    Anderson Rocha-Buelvas

    2013-09-01

    Full Text Available Objetivo: identificar los factores determinantes de la utilización de los servicios de salud bucal en hombres y mujeres universitarios del municipio de Pasto, Departamento de Nariño (Colombia. Metodología: en una muestra de 338 estudiantes de una universidad, se aplicó una encuesta confidencial, elaborada con base en estudios previos utilizando un modelo comportamental de uso de servicios de salud. Resultados: la prevalencia de utilización de los servicios de salud bucal fue del 57%. Se encontró con respecto a factores de predisposición, que más de la mitad de la población encuestada eran adultos jóvenes entre 20-24 años; que asistían más a la consulta los estudiantes de semestres académicos superiores y menos los de mayor edad; que la mitad pertenecían a un estrato socioeconómico medio y que una décima parte poseían un bajo apoyo social. Se encontró sobre factores de capacidad que las tres cuartas partes que respondieron tener un buen estado de salud, eran los que más utilizaban los servicios de salud bucal; que los que respondieron estar satisfechos con la apariencia de sus dientes, eran los que menos utilizaban los servicios de salud bucal; que una tercera parte había tenido un mal estado de salud bucodental y que casi la mitad tuvo problemas dentales. Sobre los factores de necesidad se encontró que cuando un estudiante tiene afectada su calidad de vida por minusvalía asiste más a la consulta odontológica en el último año. Conclusión: existen leves diferencias por sexo en la utilización de los servicios de salud bucal y que si bien en esta población el resultado fue mayor que en otros estudios, no necesariamente este resultado garantiza que tengan una mejor salud bucal. Objective: to identify the determinant factors of dental health service use among university students in the municipality of Pasto (located in the Nariño Department, Colombia. Methodology: a confidential survey was used on a sample of 338

  6. Déficit urbano-habitacional: Una aplicación a la comuna tres del municipio de Pasto, año 2011

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    Bayron Alberto Paz Noguera

    2012-01-01

    Full Text Available El artículo pretende aportar a la validación de teorías sobre la estimación integral de deficiencias habitacionales que el déficit convencional de vivienda no logra capturar. La hipótesis fundamental es que las condiciones habitacionales evaluadas según el impacto en la calidad de vida, no se pueden considerar en ausencia de factores que afectan externamente al hogar, pues el hombre es un ser que encuentra su bienestar en la interacción con sus pares y el entorno. Se propone la estimación del Déficit Urbano-Habitacional que incluye la valoración del déficit de vivienda, pero también de la infraestructura, los servicios y el espacio público. Para validar lo anterior, se realizó una aplicación a la Comuna Tres del Municipio de Pasto. Los resultados indican que de los cuatro componentes que integran el Déficit Urbano-Habitacional, el déficit de servicios y el de espacios públicos resultan ser los más preocupantes.

  7. Evaluación agronómica y nutricional del pasto EStrella africana (Cynodon nlemfuensis en la zona de monteverde, puntarenas, Costa Rica. I. DISPONIBILIDAD DE BIOMASA Y FENOLOGÍA

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    Luis Villalobos

    2013-01-01

    Full Text Available Se evaluó la disponibilidad de biomasa y la fenología del pasto estrella africana (Cynodon nlemfuensis a lo largo de 2 años en muestreos bimensuales, en 4 fincas comerciales de ganado lechero ubicadas en los cantones de Tilarán y Central (latitud 10°20’ N, longitud 84°50’ O,altitud 800 a 1200 msnm de las provincias de Guanacaste y Puntarenas, respectivamente. La disponibilidad de materia seca pre-pastoreo y laedad fenológica promedio fueron 4484 kg.ha-1. corte-1 y 7,36 hojas verdes por rebrote, respec- tivamente. La composición botánica promediode las pasturas fue 86,81% estrella, 2,52% otrasgramíneas, 1,39% leguminosas, 1,53% malezas y 7,75% material senescente. La disponibilidad de biomasa fue mayor en las fincas con influencia climática del Oceano Pacífico y su producción disminuyó en los meses de mayor precipitación. La edad fenológica del pasto estrella africana se ubica entre 6 y 8 hojas verdes por rebrote, lo cual permite una adecuada recuperación del pasto, y disminuyó en los meses con excesos deprecipitación.

  8. Evaluación agronómica y nutricional del pasto elefante (pennisetum purpureum) a partir de diferentes biofertilizantes en la Finca Los robles de la Fundación Universitaria de Popayán

    OpenAIRE

    Suárez Ramos, Claudia Andrea

    2016-01-01

    Tesis (Maestría en Desarrollo Sostenible y Medio Ambiente). Universidad de Manizales. Facultad de Ciencias Contables Económicas y Administrativas, 2016 En Colombia, el pasto elefante (Pennisetum purpureum) se ha introducido como una alternativa de alimentación animal que pueda satisfacer las necesidades nutricionales y por ser una especie de crecimiento resistente a condiciones extremas de temperatura. El presente documento reporta los resultados del trabajo de investigación titulado “Eva...

  9. Evaluación agronómica y nutricional del pasto Ryegrass perenne tetraploide (Lolium perenne producido en lecherías de las zonas altas de Costa Rica. II. Valor nutricional

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    Luis Villalobos

    2010-01-01

    Full Text Available Se determinó el valor nutricional del pasto ryegrass perenne tetraploide (Lolium perenne en 4 fincas comerciales de ganado lechero ubicadas en Chicuá de Oreamuno (latitud 09°59', longitud 83°52', altitud 3090 msnm, provincia de Cartago. Las muestras se tomaron cada 2 meses a una altura de cosecha de 10 cm sobre el suelo, simulando el pastoreo que realizan los animales durante un período de un año. La composición nutricional promedio anual fue de 25,21% PC, 46,26% FDN, 25,57% FDA, 3,29% lignina, 15,40% CNF y 77,95% DIVMS y su contenido energético, expresado como TND, ED, EM, ENL (3X y ENG, fue 61,95%, 2,92, 2,45, 1,53 y 0,92 Mcal.kg-1 de MS, respectivamente. El valor nutricional del pasto ryegrass perenne varió (p¿0,05 según la época del año y el manejo en finca, especialmente en lo referente al período de recuperación de las pasturas. Este pasto tiene un contenido alto de PC y CNF, que permite a nivel ruminal la producción de proteína microbial, la cual es la mejor proteína que puede consumir un rumiante. En general, el valor nutricional del pasto Ryegrass perenne producido a altitudes superiores a los 2500 msnm en Costa Rica es similar, al producido en zonas de clima templado de donde es originario.

  10. MUJERES COLONIALES AL FILO DE SU MUERTE: ECONOMÍA Y CULTURA EN LOS TESTAMENTOS DE MUJERES DE PASTO A FINES DEL SIGLO XVIII

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    Alcira Dueñas Martínez

    2000-11-01

    Full Text Available El propósito de este ensayo es la exploración de algunos testamentos de mujeres de variada procedencia social, en un área que fue frontera geopolítica tanto durante el imperio incaico como bajo el dominio colonial español: Pasto en el siglo XVIII (en el presente es la frontera entre las naciones modernas de Colombia y Ecuador. Enfocaré el testamento como un texto literario, no como una evidencia empírica para el estudio histórico. En tal condición, espero identificar huellas que me permitan reconstruir las mujeres que los inspiraron como sujetos de enunciación en sus diferentes posiciones dentro de la economía, la cultura y la sociedad de la época. Este ensayo explora además la auto-percepción de las mujeres testantes y su forma peculiar de asumir los roles que la sociedad colonial les asignaba. A pesar de que en la segunda mitad del siglo XVIII las sociedades indígenas se habían involucrado ya en un avanzado proceso de transculturación, los testamentos estudiados permiten apreciar cómo en dicho proceso persiste una latencia de andinidad. Si bien las convenciones hispánicas de propiedad privada, herencia y prestigio, son dimensiones prominentes del testamento, no lo son menos, por aparecer en forma velada y a veces en franca evidencia, los conceptos andinos de herencia matrilineal y la connotación de poder asociada a las prendas de vestir, y la solidaridad entre mujeres, por ejemplo.

  11. DE LA PERTENENCIA SOCIAL Y LA PERTINENCIA ACADÉMICA DEL CURRÍCULO DEL PROGRAMA DE LICENCIATURA EN ARTES VISUALES DE LA UNIVERSIDAD DE NARIÑO DE LA CIUDAD DE SAN JUAN DE PASTO-COLOMBIA

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    Bernarda Elisa Pupiales Rueda

    2012-07-01

    Full Text Available El artículo es el resultado de la investigación realizada en el año 2010, cuyo objetivo se orientó a identificar si el Currículo del Programa de Licenciatura en Artes Visuales de la Universidad de Nariño, San Juan de Pasto, Colombia, tiene en cuenta los aspectos que determinan la pertenencia social y la pertinencia académica, para lo cual se realizó una investigación etnográfica a partir entrevistas a docentes y encuestas a estudiantes, además se realizó un análisis del currículo teórico y del Plan de Desarrollo de la Universidad de Nariño 2008-2020 pensar la Universidad y la Región. La muestra estuvo conformada por treinta y seis (36 estudiantes, de un total de ciento treinta y cuatro (134 matriculados en el programa y por veintidós (22 docentes, de los veinticuatro (24 del total, además de siete (7 miembros del Comité Curricular del Programa.

  12. Efecto del tipo de desmonte sobre la descomposición de pastos nativos e introducidos en el chaco Árido de la Argentina

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    ABRIL, A

    2012-04-01

    Full Text Available ResumenLa dinámica de la descomposición y la liberación de nutrientes a partir de los restos vegetales son procesos clave para la fertilidad edáfica, por lo que resultan adecuados para estimar el grado de sustentabilidad de los sistemas productivos. En este trabajo se evaluó el efecto del tipo de desmonte (total y selectivo sobre la tasa de descomposición, la liberación de N y la fertilidad del suelo de pastizales (nativos e introducidos para uso ganadero en el Chaco Árido de la Provincia de Córdoba, con la finalidad de predecir el grado de sustentabilidad de ambos sistemas productivos bajo las condiciones climáticas de la región. Se utilizó un diseño experimental de parcela dividida: desmonte (total vs selectivo/pastura (nativa vs introducida, con 4 réplicas por tratamiento.En las parcelas se colocaron jaulas de descomposición con 20 g de residuos (colectados en cada tratamiento que fueron retiradas al final de cada estación climática durante dos años. Muestras de suelo (0-20 cm de profundidad fueron tomadas anualmente en ambos tipos de desmonte y en un bosque colindante como testigo.En cada fecha de muestreo se evaluó el peso y las características químicas y biológicas de los residuos. En las muestras de suelo se determinó el contenido de nitratos, las fracciones de la materia orgánica y la respiración edáfica. Durante el primer año de estudio, la tasa de descomposición y la mayoría de los parámetros analizadosfueron muy semejantes entre tratamientos. Contrariamente, en el segundo año todos los valores fueron muy erráticos debido a que germinaron pastos dentro de las jaulas. Los parámetros de fertilidad analizados no mostraron diferencias entre sistemas de desmonte y se correlacionaron con el contenido de humedad. Nuestros resultados indican que: a las condiciones climáticas del Chaco Árido afectan la descomposición en mayor medida que la composición química de los residuos y el tipo de desmonte; b, los

  13. Generación de pagos por certificados de reducción de emisiones, bajo el mecanismo de desarrollo limpio para cercos vivos en la cuenca alta del Río Pasto

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    Javier Sabogal-Aguilar

    2007-01-01

    Full Text Available Este artículo es resultado del proyecto de Restauración y Protección de Agroecosistemas Estratégicos en la Captura de Carbono, Municipio de Pasto, Nariño. Explora la posibilidad que tienen las comunidades y propietarios de pequeños predios de la Cuenca Alta del Río Pasto de contribuir a la mitigación del Cambio Climático a la vez que obtienen ingresos monetarios y otros benefi cios por la participación en proyectos del Mecanismo de Desarrollo Limpio forestales. El documento presenta una metodología novedosa de participación de comunidades de bajos ingresos en proyec tos MDL forestales que se diferencia de las existentes por el alto contenido de participación y confi anza generada entre los pequeños productores y las instituciones desarrolladoras. Se concluye con la evidencia de viabilidad del proyecto MDL forestal planteado y los benefi cios que generó el proyecto en términos académicos, de desarrollo comunitario y de generación de potenciales proyectos que pueden ser desarrollados con posterioridad.

  14. Software educativo “Mundo Agroforestal”: estudio de caso, subcuenca alta del río Pasto, Nariño, Colombia

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    Ángela Lucía León

    2014-06-01

    Full Text Available Las Tecnologías de la Información y la Comunicación (TIC, son herramientas que han permitido evolucionar en el proceso de enseñanza aprendizaje, innovación, difusión de información, adquisición y generación de conocimiento. El objetivo de la investigación fue desarrollar un “Software Educativo Agroforestal - SEAf”, dirigido a jóvenes de instituciones educativas rurales, como estrategia pedagógica para la enseñanza agroforestal. Mediante Investigación Acción Participativa (IAP, se diagnosticó el conocimiento agroforestal de estudiantes de dos instituciones educativas municipales de la subcuenca alta del río Pasto, implementando talleres participativos, encuestas semi-estructuradas y mapas parlantes; se realizó un análisis de correspondencias múltiples (ACM, que permitió diseñar y estructurar el SEAf con el programa Flash Cs4, el cual fue evaluado con pruebas Alfa y Beta. Como resultado se logró diseñar un software educativo agroforestal denominado “Mundo Agroforestal” que incluyó los gustos y preferencias de los jóvenes; de acuerdo al color, actividades lúdicas, paisajes y personajes animados que fueron plasmados en las interfaces. El software fue altamente aceptado (94% respecto a las variables diseño, facilidad de navegación, pertinencia de color, temáticas abordadas e ilustraciones; lo que permitió la validación de éste.

  15. PRODUCCIÓN DE CARNE EN NOVILLOS F1 ROMO-CEBÚ CON PASTO ANGLETON (Dichanthium aristatum ENSILAJES Y SUPLEMENTOS EN EL VALLE DEL SINÚ

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    Lino Torregroza S

    2006-12-01

    Full Text Available comercial bajo condiciones de manejo eficiente de pasturas y de suplementación estratégica durantela época seca. Materiales y métodos: Se evaluó la producción de carne de novillos F1 romo x cebúy cebú en pastoreo rotacional de pasto angleton bajo condiciones del Valle del Sinú, departamentode Córdoba. El experimento tuvo una duración de 289 días que incluyó un período completo desequía. Durante la época de lluvia, los animales se suplementaron con un kilo de semilla de algodónpor día, y en la época seca, además del pastoreo, se suministró, en promedio, 10 kilos de ensilajede maíz balanceado con .02% de urea-sulfato de amonio y suplementado con un kilo de semilla dealgodón más medio kilo de salvado de arroz. Resultados. En la pastura se estimó la producción demateria seca, la composición química y la digestibilidad In Situ de la materia seca. La carga fue de4 animales por hectárea los cuales se pesaron cada 24 días. La ganancia promedia diaria fue de0.660 y 0.728 kilos/animal/día, respectivamente para los F1 y cebú, sin diferencia estadística(p>0.05. Conclusión. La producción de carne por hectárea año fue de 963.6 y 1062.8 kilogramos,para F1 y cebú respectivamente. El valor de producción de un kilo de carne fue de US$ 0.38 paralos F1 y US$ 0.34 para los cebú, lo que demustra una buena rentabilidad

  16. Efecto de la temperatura y el pH en la producción de biomasa de Azospirillum brasilense C16 aislada de pasto guinea

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    F Romero-Perdomo

    Full Text Available El objetivo del estudio fue evaluar el efecto de la temperatura y el pH en la viabilidad celular de la cepa Azospirillum brasilense C16. Se estudiaron cinco temperaturas (entre 28 y 36°C y cinco pH (de 4,5 a 8,5. El efecto de la temperatura se evaluó mediante la medición del crecimiento radial (mm de la cepa, sobre un papel de filtro en medio batata. En el caso del pH se utilizó un fermentador de tanque agitado -con una configuración tipo Rushton- para cuantificar la velocidad de crecimiento, la producción de biomasa y el tiempo de duplicación de la cepa; las condiciones de fermentación fueron: 400 rpm, 1 Lpm y 30°C, durante 24 horas. El pH y la temperatura influyeron significativamente (p < 0,05 en la producción de A. brasilense C16. La temperatura de 30°C fue la más favorable para que se multiplicara la cepa, con 23,21 mm de crecimiento radial; mientras que la mayor o igual a 34°C inhibió su crecimiento. Los mejores resultados se obtuvieron con un pH de 6,8, con diferencias significativas (p < 0,05 respecto al resto. Con esta condición se obtuvieron los valores más altos de velocidad de crecimiento (1,79 h-1 y producción de biomasa (8,65 log10 UFC mL-1, y el valor más bajo del tiempo de duplicación (1,09 h-1. Estos resultados poseen aplicabilidad biotecnológica, y son de gran importancia en el momento de definir y controlar las condiciones de producción masiva de biomasa de A. brasilense C16 para futuras formulaciones como biofertilizante en diversos cultivos de interés en Colombia.

  17. Fertilización de pastos en zonas semiáridas de secano del S-W- español

    OpenAIRE

    Olea Márquez de Prado, L.; Jiménez Mozo, J.

    2011-01-01

    Se presentan los resultados de aportaciones de P, K, Mg + Ca, Mo + Mn y Cu + Co sobre pastizales artificiales de leguminosas + gramíneas y pastos naturales en varias zonas de Extremadura. En pastizales artificiales la fertilización previa al establecimiento con 75-95 u. de P2O5 /Ha, duplica la producción. En pastos naturales la fertilización anual con 46 u. de P2 O5, duplica la producción y el porcentaje de leguminosas. Los mayores incrementos se consiguen con aportaciones anuales de 40 u. de...

  18. Efectividad de métodos de control de malezas en la producción de forraje del pasto Pangola (Digitaria decumbens Stent.

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    Valent\\u00EDn Alberto Esqueda-Esquivel

    2007-01-01

    Full Text Available Efectividad de métodos de con trol de ma le zas en la producción de forraje del pasto Pangola (Digitaria decumbens Stent. En el periodo de agosto de 1998 a agosto de 1999 se condujo un experimento en Emilio Carranza, municipio de Ve ga de Alatorre, Ve racruz, México, con el objeto de determinar el efec to de tra ta mien tos de con trol quí mi co y ma nual de ma le zas en la pro duc ción de fo rraje de zacate Pangola y en la den si dad de po bla ción de ma le zas presentes al inicio del siguiente ciclo de lluvias. Se utilizó el diseño experimental de blo ques completos al azar con cua tro re pe ti cio nes. Los tra tamien tos evaluados fueron: 1. Mezcla for mulada de picloram + 2,4-D (64 + 240 g/100 l agua 2. Mez cla for mu la da de pi cloram + flu ro xi pir (40 + 40 g/100 l agua 3. Con trol ma nual mediante chapeo con machete y 4. Testigo enhierbado. Las parcelas experimentales tuvieron 30 m de longitud y 10 m de anchura. Los tra ta mien tos 1, 2 y 3 se apli ca ron el 17 de agos to de 1998, cuan do la zar za de lo ma (Mimosa albida, que fue la es pe cie de ma le za do mi nan te te nía en tre 40 y 60 cm de al tu ra y la cobertura promedio de malezas del lote experimental, era de 57,9%. Se eva luó la co ber tu ra de ma le zas y pas to y el control de ma le zas a los 29, 60, 80, 114 y 154 días des pués de la aplicación de los tratamientos (DDA. También se determinó la producción de forraje de za ca te Pan go la a los 45, 80, 143 y 178 DDA. Con pi clo ram + flu ro xi pir y pi clo ram + 2,4-D, se controló más eficientemente a M. albida y el com ple jo de ma le zas her bá ceas que con el cha peo y se ob tu vo una produc ción significativamente mayor de ma teria seca de pas to. Ambos tratamientos herbicidas presentaron menor densidad de po bla ción de ma le zas en el si guien te ci clo de llu vias que el tratamiento con chapeo.

  19. Bacterias celulolíticas aisladas del intestino de termitas (Nasutitermes nigriceps con características probióticas y potencial en la degradación del pasto

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    Cecilia Lara Mantilla

    2013-06-01

    Full Text Available Título corto: Bacterias celulolíticas aisladas del intestino de termitas (Nasutitermes nigriceps  Título en ingles:  Cellulolytic bacteria isolated from  termites’ gut (Nasutitermes nigriceps with probiotic characteristics and potential pasture degradationResumen: El objetivo de la presente investigación fue aislar bacterias celulolíticas del intestino de termitas (Nasutitermes nigriceps para determinar sus propiedades probióticas in vitro y su potencial en la degradación de pasto. Las termitas fueron tratadas con detergente antibacterial, separando y macerando luego, el intestino de las mismas en agua peptonada estéril. Diluciones de esta mezcla fueron inoculadas en cajas Petri con medio Luria Bertani (LB y Carboximetilcelulosa (CMC al 2%, incubando a 37º C por 24 horas, para luego revelar con Rojo Congo al 1%. Las bacterias que presentaron mayores halos de degradación fueron sometidas a tinción de Gram y a pruebas probióticas de temperatura, pH, salinidad y presencia de sales biliares, así como también a pruebas de antagonismo y degradación de pasto. Los resultados revelaron la presencia de 9 bacilos celulolíticos Gram (- de los cuales, los bacilos BTN7 y BTN8, presentaron los mejores halos de degradación, 12 y 14 mm de diámetro respectivamente, creciendo adecuadamente en las diferentes pruebas probióticas con densidades entre 106 y 108 UFC/ml; el porcentaje de degradación de materia seca fue de 39.73% y 36.10% en 48 y 72 horas respectivamente.  Las pruebas bioquímicas API E (bioMérieux SA revelaron que los bacilos BTN7 y BTN8 pertenecen al género Enterobacter sp. Los anteriores resultados abren la posibilidad de emplear, estos microorganismos como aditivos en la alimentación de rumiantes, a fin de contribuir con un mayor aprovechamiento de pastos, y otros sustratos vegetales lignocelulósicos.Palabras claves: Probióticos, Maralfalfa (Pennisetum sp., microorganismos ruminales, lignocelulosa, Enterobacter

  20. Fertilidad y extracción de nutrientes en la asociación maíz-pastos en suelos ácidos del piedemonte Llanero de Colombia

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    Rincón Álvaro

    2008-08-01

    Full Text Available

    En un oxisol del Centro de Investigaciones La Libertad de Corpoica, a 17 km de Villavicencio, se estableció la asociación de maíz con los pastos B. decumbens, Mulato 1 y Toledo, como medio para recobrar la productividad de una pradera degradada. Los minerales aplicados 45 días antes de la siembra fueron: Ca, 555 kg ha-1; Mg, 88 kg ha-1; P, 35 kg ha-1 y S, 45 kg ha- 1, utilizando como fuente cal dolomítica, roca fosfórica y yeso agrícola; al momento de la siembra se aplicaron: 31 kg ha-1 de P, 27 de N (fuente fosfato diamónico; 37,5 de K (fuente cloruro de potasio; 3 de Zn; 0,1 de Cu; 0,5 de B y 1,2 de S (fuente borozinco. A los 15 después de la siembra (dds se aplicaron 37,5 kg ha-1 de K. La aplicación de N se fraccionó en dosis de 100 y 200 kg ha-1 a los 15 y 35 dds, respectivamente. Los análisis de suelos realizados después de la cosecha del maíz demostraron un mejoramiento de la fertilidad, ya que la saturación de bases aumentó de 24% inicial a 47%, el P disponible pasó de 2 ppm a 11,8 ppm, el S aumentó de 3 ppm a 6 ppm. En el momento de la cosecha, la mayor extracción de N fue la hicieron el grano de maíz y los pastos (43 y 45 kg ha-1, respectivamente, mientras que el P se presentó en mayor concentración en el grano de maíz (12,7 kg ha-1. Los pastos y la soca de maíz (tallos y hojas después de la cosecha se caracterizaron por un mayor contenido de K (40 y 27 kg ha-1, respectivamente. El Ca, Mg y S fueron más extraídos por los pastos (9; 8 y 6 kg ha-1, respectivamente; los minerales extraídos en mayor cantidad por el maíz y los pastos fueron N (91%, P (57% y K (58%, mientras que los de menor extracción fueron Ca (2,2% y Mg (16%.

  1. Infección por VPH en mujeres del municipio de Pasto (Colombia) con resultados de citología normal

    OpenAIRE

    Sánchez-Ortega, Claudia; Suárez, Narváez Karen; Yépez-Chamorro, Maria; Guerrero-Flórez, Milena

    2013-01-01

    Introducción. El cáncer de cuello uterino (CCU) asociado a la infección por VPH se considera un importante problema de salud pública a nivel mundial. En Colombia, es la segunda causa de muerte por cáncer en mujeres. En el municipio de Pasto (N) ubicado al suroccidente de Colombia, la tasa de incidencia es de 27,3/100.000 habitantes. Profundizar en la historia natural de la infección por VPH permitiría mejorar las estrategias de control y diagnóstico temprano para evitar la progresión de este ...

  2. Efectos del antecedente cultural en las micorrizas nativas y la productividad del pasto brachiaria (Brachiaria decumbens cv. Señal Effects of the cultural antecedent on native mycorhizae and the productivity of the brachiaria pasture (Brachiaria decumbens cv. Señal

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    P.J Gonzále

    2007-03-01

    Full Text Available Se evaluó la influencia del antecedente cultural en la micorrización nativa y en el rendimiento del pasto brachiaria (Brachiaria decumbens cv. Señal; para ello se seleccionaron dos lotes de 0,5 ha, los cuales durante cinco años previos a la siembra del pasto estuvieron ocupados, en un caso por cultivos de ciclo corto (granos, viandas y hortalizas y en otro por pastos naturales permanentes (principalmente especies del complejo Bothriochloa-Dichanthium y Teramnus labialis. En cada lote se delimitaron diez parcelas de 200 m² cada una, y en mayo de 2004 se sembró la brachiaria en surcos separados a 70 cm y a chorrillo, con una dosis de 8 kg de semilla total ha-1. Después del establecimiento el pasto se cortó cada seis y ocho semanas en los períodos lluvioso y poco lluvioso, respectivamente, y en ningún momento se aplicaron fertilizantes químicos u orgánicos. En cada parcela se tomaron muestras de raíces y de suelo de la rizosfera, para determinar las estructuras micorrízicas del pasto (porcentaje de colonización, porcentaje de densidad visual y número de esporas por 100 g, así como muestras de la biomasa aérea para determinar el contenido y la extracción de macronutrientes, y el rendimiento de MS. Las parcelas que tuvieron los pastos naturales como cultivo precedente mostraron valores significativamente mayores (PThe influence of the cultural antecedent on native mycorhization and the yield of the brachiaria pasture (Brachiaria decumbens cv. Señal was evaluated; for that two 0,5 ha lots were selected, which were occupied for five years before sowing the pasture, in one case by short cycle crops (grains, tubers and vegetables and in the other by permanent natural pastures (mainly species of the Bothriochloa-Dichanthium complex and Teramnus labialis. In each lot ten plots of 200 m² each were delimited, and in May, 2004, the brachiaria was sown in rows separated by 70 cm and with drilling, with a dose of 8 kg total seed ha-1. After

  3. LOS MACROINVERTEBRADOS COMO INDICADORES DE LA CALIDAD DEL SUELO EN CULTIVOS DE MORA, PASTO Y AGUACATE THE MACROINVERTEBRATES AS INDICATORS OF THE QUALITY OF SOIL IN BLACKBERRY, GRASS AND AVOCADO CROPS

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    Santiago Rendón Pareja

    2011-06-01

    Full Text Available El estado de las propiedades dinámicas del suelo, tales como el contenido de la materia orgánica, la diversidad de organismos, o los productos microbianos en un tiempo particular, permiten inferir sobre la calidad del suelo. Los indicadores disponibles para evaluarla, pueden variar entre localidades, dependiendo del tipo y uso del suelo, función y factores de formación del mismo. Los invertebrados se pueden constituir en indicadores de la calidad de un suelo, dado que juegan un papel vital en los procesos de ciclaje de nutrientes; además, su diversidad, número y funciones son sensibles al cambio ambiental en las condiciones del suelo, asociadas con actividades propias en los agroecosistemas. Con el propósito de cualificar la calidad del suelo en varios sistemas productivos, se evaluó la presencia de macroinvertebrados en cultivos de mora, pasto y aguacate, empleando para el muestreo la técnica del monolito propuesto por Instituto de Fertilidad y Biología de Suelos Tropicales (TSBF y luego se procedió a identificarlos a nivel de familia. La mayor cantidad de macroinvertebrados se encontró en los primeros 10 cm, siendo el cultivo de mora el que registró la mayor diversidad.The state of soil dynamic properties, such as organic matter content, diversity of organisms, or microbial products in a particular time, allow to infer about soil quality. The indicators available to evaluate, may vary among locations, depending on the type and land use, function and factors of formation. Invertebrates can be indicators of soil quality, as they play a vital role in nutrient cycling processes, furthermore, their diversity, number and functions are sensitive to environmental change in soil conditions associated with activities own in agroecosystems. In order to qualify the quality of soil in various production systems was evaluated the macroinvertebrates presence in mulberry, pasture and avocado crops, using the monolith sampling technique proposed

  4. Ensayo de fertilización del pasto puntero (Hyparrhenia rufa con un diseño de superficies de respuesta en la zona de Chaparral, Tolima

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    Buritica Martínez Hernando

    1991-12-01

    Full Text Available Se realizó un esayo de fertilización con NPK empleando urea, superfosfato tripley cloruro de potasio como fuentes en pasto puntero (Hyparrhenia rufa en la vereda Espíritu Santo, municipio de Chaparral, Tolima en suelos francos arenosos de laderade baja a mediana fertilidad natural, altura de 1.000 m.s.n.m., temperatura diaria de 25 grados, precipitación promedia anual de 2.500 mms. y una estación seca bien definida entre los meses de junio a septiembre, utilizando el modelo de superficies de respuesta desarrollado por Box y Hunter, ex·puesto por Cochran y Cox y recomendado por Instituto Colombiano Agropecuario. Los modelos de regresión obtenidos, muestran incrementos crecientes en la producción de forraje verde y contenido proteínico para las aplicaciones crecientes de nitrógeno y fósforo, con máximos equivalentes a 41 toneladas/Ha y 3.5% con aplicaciones de 400 y 200 kgs/Ha de úrea y superfosfato triple. En las condiciones de ensayo, se demuestra la conveniencia de la aplicaciónde nitrógeno y fósforo en cantidades comprendidas entre 150 a 200 y 75 a too kilos de úrea y superfosfato triple por hectárea respectivamente.
    A field experiment were conducted in order to know the response of "Puntero" grass (Hyparrhenia rufa to the application of diferent amount of Urea, concentrated superphosphate and muriate of potash, in a sand 108m soil of farm located in Chaparral (Tolima at 1000 above sea level, 250C annual mean temperature, and 2.500mm annual mean precipitation. Box and Hunter's surface response model was usedas advised by the Instituto Agropecuario Colombiano.The regresion analysis shows that increasing applications levels of Nitrogen (urea and Phosphorus (superphosphate increase the amount of green forage and protein conten; highest level of forage 41 ton and protein 3.5% content were obtained using 400 and 200 kg.ha-1 of urea and superphosphate. From this information, the author recomend the use of amounts between 150

  5. Evaluación del pretratamiento con ácido sulfúrico diluido del pasto maralfalfa (Pennisetum glaucum x Pennisetum purpureum para la producción de etanol

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    Lady Mateus

    2012-01-01

    Full Text Available Título en ingles: Dilute sulfuric acid pretreatment of goliath grass (Pennisetum glaucum x Pennisetum purpureum for ethanol cellulosic Resumen : Evaluar la producción de etanol a partir de cultivos lignocelulósicos, específicamente pastos de rápido crecimiento en la región, constituye una alternativa a la demanda de biocombustibles. En la presente investigación se seleccionó el pasto Maralfalfa (Pennisetum  glaucum x Pennisetum  purpureum utilizando el pretratamiento con ácido sulfúrico diluido a diferentes temperaturas (110, 130, 150, 170 y 190 °C y concentraciones de ácido (0.8, 1.2 y 2.0% (p/p, seguido de un proceso de hidrólisis enzimática utilizando celulasas y celobiosas comerciales y un proceso de hidrólisis y fermentación simultanea. La máxima producción de etanol obtenido fue 117 mg etanol/ g biomasa pretratada a 190 °C y 1,2 %(p/p de ácido sulfúrico. El líquido hidrolizado fue caracterizado calculando el porcentaje de glucosa, xilosa y lignina solubilizadas y degradadas durante el pretratamiento. Palabras clave: etanol lignocelulósico; celulosa; hemicelulosa. Abstract: The goliath grass (Pennisetum  glaucum x Pennisetum  purpureum was pretreated with different sulfuric acid concentrations (0.8, 1.2 y 2.0% (w/w from low to high temperatures (110, 130, 150, 170 y 190 °C followed by enzymatic hydrolysis and SSF of remaining solids. The maximum yield was 117 mg of ethanol/g biomass to 190 °C and 1.2 % (w/w of sulfuric acid. Key words: lignocellulosic ethanol; hemicelluloses; cellulose.

  6. Evaluación agronómica y nutricional del pasto Ryegrass perenne tetraploide (Lolium perenne producido en lecherías de las zonas altas de Costa Rica. I. Producción de biomasa y fenología

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    Luis Villalobos

    2010-01-01

    3090 msnm, provincia de Cartago. La disponibilidad de materia seca pre y pos-pastoreo, el aprovechamiento por hectárea y su porcentaje de utilización promedio para todas las fincas fueron 4110, 2276 y 1833 kg.ha-1 por corte y 44,82%, respectivamente. La edad fenológica y la relación hoja: tallo promedio de las pasturas de ryegrass perenne fueron 2,84 hojas verdes por rebrote, 56:44. Asimismo, la composición promedio de la pradera fue 76,09% ryegrass, 13,76% otras gramíneas (principalmente pasto kikuyo, 6,02% trébol, 1,25% malezas y 2,88% material senescente, respectivamente. La mayor producción de biomasa durante marzo y abril puede conservarse para utilizarse durante las épocas críticas de disponibilidad de forraje o cuando por razones climatológicas se reduce el número de horas efectivas de pastoreo. La evaluación de la edad fenológica en que se pastorea el pasto ryegrass perenne, indica que las fincas en la zona de estudio utilizan esta gramínea en un estado en el que sus reservas de carbohidratos se han recuperado, lo que permite obtener una producción de biomasa y valor nutricional adecuados para satisfacer las necesidades del ganado lechero de la zona.

  7. USO DE LA PLATAFORMA VIRTUAL DEL RECINTO URACCAN-NUEVA GUINEA, II SEMESTRE 2012

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    Margarita Arastenia Zamora Huete

    2013-10-01

    Full Text Available Se valora el uso de la plataforma virtual del recinto URACCAN-Nueva Guinea, en el área  de Ciencias Administrativas e Informática, implementada durante el II semestre del 2012. Se desarrolló bajo un enfoque cuantitativo empleando la encuesta como técnica de recolección de datos y entrevistas a fuentes específicas. La investigación da mayor relevancia a la plataforma virtual Moodle y sus características por ser el software educativo utilizado por el recinto universitario para desarrollar los procesos educativos en los entornos virtuales.Los resultados muestran el poco uso de la plataforma virtual, tanto de estudiantes y docentes, aunque la universidad ha realizado esfuerzo por capacitar a los docentes en metodologías a través de entornos virtuales; empero, el diseño de los cursos por algunos docentes no incentiva su uso: son pocos atractivos y no se aprovechan todas las opciones que la plataforma otorga para su uso eficiente. Los estudiantes y docentes ven con buenas expectativas y aprueban de manera complementaria la utilización de plataformas virtuales para el desarrollo de sus clases, ya que existen pocas condiciones en la universidad en cuanto al acceso y equipos para su implementación.SummaryThis study was carried out in order to evaluate the virtual platform use in URACCAN-Nueva Guinea campus, in the area of Administrative Sciences and Informatics, implemented during the second semester of the year 2012. It was developed with a quantitative approach using the survey as a technique for data collection and interviews with specific sources. The research gives major importance to the Moodle virtual platform and its characteristics, for being the educational software used by the campus to develop educational processes in virtual environments.The results show little use of the virtual platform, both by students and teachers, although the university has made efforts to train teachers in methodologies through virtual environments

  8. Biodiversidad íctica de praderas de pasto marino de la costa noroeste del Golfo de Cariaco, Venezuela Ichthyc biodiversity of seagrass meadows from the Northwest coast of Cariaco Gulf, Venezuela

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    Luis Alejandro Ariza A

    2012-06-01

    Full Text Available Los pastos marinos son ecosistemas costeros de alta productividad, con gran diversidad y abundancia de peces, la cual es aprovechada por pescadores artesanales. En este estudio se analizó la estructura comunitaria íctica de praderas de Thalassia testudinum en Manzanillo (M y La Brea (LB, costa noroeste del Golfo de Cariaco, Venezuela. También, se realizaron muestreos mensuales (11-2006 al 10-2007, con la utilización de una red playera. Se capturaron 34 810 organismos agrupados en 13 órdenes, 36 familias y 83 especies. En ambas zonas, el número de especies fue similar, aunque el número de organismos vario, y se encontró para M un total de 55 especies y 13 210 organismos y para LB 58 especies y 21 600 organismos. Las especies más abundantes y de mayor biomasa en el área muestreada fueron: Nicholsina usta, Haemulon boschmae, H. steindachneri, Harengula jaguana, Halichoeres bivittatus y Hemiramphus brasiliensis. Los visitantes ocasionales fue el componente comunitario más frecuente con 59%, los cíclicos y los residentes permanentes obtuvieron 22 y 19%, respectivamente. En M la H’n fue de 1.71±0.64bits/ind; entretanto en LB fue 1.95±0.51bits/ind. Los valores de la diversidad estuvieron relacionados directamente con la equitabilidad e inversamente con la dominancia. Los bajos valores del índice de similaridad, entre localidades permite establecer que estas comunidades ícticas son disimiles, debido quizás a la estructuración de cada pradera de Thalassia y a la conectividad con otros sistemas.Ichthyc biodiversity of seagrass meadows from the Northwest coast of Cariaco Gulf , Venezuela. Seagrasses are highly productive coastal ecosystems with a high diversity and abundance of fishes, very important to support artisanal fisheries. We analyzed the fish community structure of Thalassia testudinum in the communities of Manzanillo (M and La Brea (LB, Northwest coast of Cariaco Gulf, Venezuela. Samples were taken monthly (Nov. 2006-Oct

  9. Inhibiton of Yellow Nutsedge (Cyperus esculentus L. and Bermudagrass (Cynodon dactylon (L. Pers by a Mulch Derived from Rye (Secale cereale L. in grapevines Inhibición del Crecimiento de Chufa (Cyperus esculentus L. y Pasto Bermuda (Cynodon dactylon (L. Pers. con mulch Vegetal Proveniente de Centeno (Secale cereale L. en Vides

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    Juan Ormeño-Núñez

    2008-09-01

    . Las siegas sucesivas del centeno formaron un mulch vegetal denso y duradero sobre las hileras de vides. El mulch orgánico fue un 81 y 82% más efectivo para controlar chufa y pasto bermuda, respectivamente, que el control químico (sobre la hilera + mecánico (entre las hileras. Dos aplicaciones de glifosato (octubre/diciembre al 2% para chufa y 1% para pasto bermuda no fueron suficientes para controlar efectivamente las dos malezas. El grado de control del total de malezas fue 67,3 y 43,0% más efectivo con el mulch vegetal que con los tratamientos químicos en Los Andes y Santiago, respectivamente. El alto control ejercido por el mulch de centeno se debió a que el crecimiento de los nuevos brotes de chufa y pasto bermuda fueron particularmente susceptibles al sombreamiento del mulch, formado antes de su emergencia primaveral y que permaneció hasta inicios del otoño, y a que la siega del centeno maximizó los efectos alelopáticos de esta variedad local de centeno. Se sugiere utilizar este tipo de manejo de las cubiertas vegetales y mulch para el control efectivo de malezas en otros frutales caducifolios tanto en huertos de agricultura convencional como orgánicos.

  10. Postratamiento en escala piloto del lixiviado del relleno sanitario Antanas (Pasto, Nariño por filtración-adsorción con arena, antracita y carbón activado

    Directory of Open Access Journals (Sweden)

    Andrés Felipe López Guerrero

    2016-06-01

    Full Text Available Esta investigación evaluó el postratamiento del lixiviado del relleno sanitario Antanas mediante tres filtros ascendentes en serie con arena, antracita y carbón activado granular. Se evaluaron rangos de tasas de filtración de 20-35, 60-100 y 150-300 m3/m2*d correspondientes a tiempos de contacto con el carbón de 48, 24 y 8 minutos. El seguimiento a cada condición se efectuó durante un mes, las remociones medias de DQO para dichas tasas y tiempos fueron 60,2, 54,3 y 31,4% respectivamente. El mejor desempeño del sistema dependió fundamentalmente del aumento del tiempo de contacto y no de la reducción de las tasas de filtración.

  11. Producción de semilla de guinea (Panicum maximum Jacq. en un sistema intensivo de ceba de ganado vacuno Seed production of Guinea grass (Panicum maximum Jacq. in an intensive cattle fattening system

    Directory of Open Access Journals (Sweden)

    G Oquend

    2008-09-01

    Full Text Available En un suelo Pardo sialítico del subtipo Cambisol cálcico, localizado en la Empresa Pecuaria «Calixto García», en la provincia de Holguín, se estudio la producción de semilla de guinea (Panicum maximum Jacq. en un sistema intensivo de ceba de ganado vacuno, en condiciones de riego. Los tratamientos fueron cinco varieda­des del pasto guinea: A Común; B Likoni; C Mombasa; D Tanzania; y E Tobiatá. Los siguientes métodos se consideraron a su vez como subtratamientos: 1 Siembra con semilla gámica; 2 Plantación por macollas; y 3 Por vía de trasplante. La carga se mantuvo ajustada a 2 UGM/ha. En la producción de semillas existieron interacciones favorables entre los métodos de siembra y las variedades: semilla gámica-guinea Likoni; maco­lla-guinea Mombasa, Tanzania y Tobiatá; trasplante-guinea Común. En todo el sistema de explotación se obtuvo un aporte adicional superior a los $1 000/ha por concepto de producción de semilla, sin afectar la producción animal, en la que se obtuvieron ganancias superiores a los 800 g/animal/día y producciones pro­medio de 46 212 t de carne en pie por ciclo de ceba. Se considera factible la producción de semilla del pasto guinea en sistemas intensivos de ceba de ganado vacuno.On a sialitic Brown soil of the calcic Cambisol subtype, located at the «Calixto García» Livestock Production Enterprise, in the Holguín province, the production of Guinea grass (Panicum maximum Jacq. was studied in an intensive cattle fattening system, with irrigation. The treatments were five varieties of Guinea grass: A Common; B Likoni; C Mombasa; D Tanzania; and E Tobiatá. The following methods were considered, in turn, sub-treatments: 1 Seeding with gamic seed; 2 Planting with tillers; and 3 Transplanting. The stocking rate remained adjusted at 2 animals/ha. In seed production there were favorable interactions between the planting methods and the varieties: gamic seed-Guinea grass Likoni; tiller-Guinea grass

  12. La utilización de la teoría de benchmarking como modelo comparativo para el plan estratégico en las pymes del sector lácteo en el municipio de San Juan de Pasto 2014-2019

    Directory of Open Access Journals (Sweden)

    Karola López López

    2016-01-01

    Full Text Available Los actuales desafíos que enfrenta el sector lácteo en el país, obliga a generar la necesidad de una planeación estratégica, como fundamento de vital importancia para el estudio a nivel no solo sectorial, sino también empresarial; teniendo en cuenta la generación de un diagnóstico, una evaluación y una propuesta de fortalecimiento a partir de herramientas basadas en la metodología de benchmarking, que permite realizar un análisis pertinente, promoviendo cambios que contribuyan al fortalecimiento de variables clave para el desarrollo de las Pymes del sector lácteo en la ciudad de San Juan de Pasto. De acuerdo con este contexto, se ha desarrollado el presente trabajo focalizándose en recopilar y explicar la información existente acerca del estado actual de la cadena láctea del departamento de Nariño a partir de: la evaluación de entornos, el análisis sectorial utilizando el modelo de Diamante y Cinco Fuerzas de Michael Porter, y la aplicación del benchmarking estratégico a nivel regional y empresarial, considerados como elementos importantes del presente análisis. En consecuencia, se pudo establecer la cadena de valor, generando unos lineamientos estratégicos que contribuyan al mejoramiento de los procesos de la planeación y control existentes en las Mipymes lácteas de la región.

  13. Evaluación de la carrera administrativa en la alcaldía de Pasto

    OpenAIRE

    Antonio Bastidas Unigarro

    2012-01-01

    Este artículo aborda la aplicación de las distintas normas de carrera administrativa (Leyes 27 de 1992, 446 de 1998 y 909 de 2004) en la Alcaldía Municipal de Pasto, en cuanto al sistema de talento humano se refiere y muestra los resultados de la investigación frente a procesos de ingreso, capacitación y estímulos, evaluación del desempeño y motivación del personal inscrito en carrera administrativa, abarcando el análisis histórico desde 1994, pero particularmente haciendo énfasis en los ...

  14. "Titán" y "Regio", variedades de pasto Buffel (Pennisetum ciliare) (L.) Link para zonas áridas y semiáridas

    OpenAIRE

    Beltrán López, Sergio; García Díaz, Carlos Alberto; Loredo Osti, Catarina; Urrutia Morales, Jorge; Hernández Alatorre, José Antonio; Gámez Vázquez, Héctor Guillermo

    2017-01-01

    RESUMEN: El pasto buffel (Pennisetum ciliare) es una gramínea perenne, originaria de Sud África, de buen valor forrajero que se adapta bien a una amplia gama de suelos y condiciones climáticas. Las variedades de pasto buffel Titán y buffel Regio provienen de colectas realizadas en el centro y norte de México. Fueron evaluadas en zonas áridas y semiáridas, en temporal y riego desde 1986 hasta la obtención del registro en el año 2008. Los registros definitivos otorgados por parte del Sistema Na...

  15. Índice de vulnerabilidad de los hogares en el municipio de Pasto, Colombia, 2012

    Directory of Open Access Journals (Sweden)

    Arsenio Hidalgo-Troya

    Full Text Available El objetivo del estudio fue medir la tasa de vulnerabilidad de las familias en el municipio de Pasto, Colombia. En una muestra de 270 familias de todos los estratos socioeconómicos, 239 en áreas urbanas y 31 en las zonas rurales, se realizó una investigación de carácter confidencial, utilizando el índice de vulnerabilidad, consistente en cinco dimensiones: demográfica, social, económica, ambiental y geográfica, así como previsión y prevención. Las familias estrato 1 y 2, así como los hogares del centro, oeste, noroeste y nordeste del área urbana del municipio, presentaron alta vulnerabilidad, incluso más que aquellas en áreas rurales. Un 50% de las familias estaban localizadas dentro de baja vulnerabilidad y un 42% estaban en nivel medio. Las familias más pobres fueron las más vulnerables, sin embargo, las familias pobres también eran vulnerables en el municipio de Pasto.

  16. EFECTO DE LA FERTILIZACIÓN NITROGENADA Y DE LA EDAD DE CORTE SOBRE LA DIGESTIBILIDAD INTESTINAL In vitro DE LA PROTEÍNA DEL PASTO KIKUYO (Pennisetum clandestinum Hochst NITROGEN FERTILIZATION EFFECT AND CUT AGE ON THE In vitro INTESTINAL DIGESTIBILITY OF KIKUYO GRASS (Pennisetum clandestinum Hochst PROTEIN

    Directory of Open Access Journals (Sweden)

    Marcelo Castañeda Colorado

    2008-12-01

    Full Text Available Con la finalidad de evaluar el efecto de la fertilización nitrogenada y de la edad de corte sobre la digestibilidad intestinal In vitro de la proteína del pasto kikuyo (Pennisetum clandestinum Hochst, se seleccionaron 16 parcelas a las cuales se les asignó uno de los siguientes tratamientos (cuatro parcelas/tratamiento: T1 (30 días de corte y 0 kg/N/Ha/Corte, T2 (60 días de corte y 0 kg/N/Ha/Corte, T3 (60 días de corte y 50 kg/N/Ha/Corte y T4 (30 días de corte y 50 kg/N/Ha/Corte. Luego de 120 días de tratamiento se recolectaron 5 submuestras de cada parcela con las que se conformó una muestra final para cada parcela en las que se analizó el contenido de proteína cruda y se realizó la prueba de degradabilidad ruminal a 16 horas y la prueba de digestibilidad intestinal In vitro de la proteína por el método de los tres pasos. Los resultados mostraron que no hubo efecto de la edad de corte, ni de la fertilización nitrogenada sobre el porcentaje de proteína cruda del pasto kikuyo. Los valores de digestibilidad intestinal como porcentaje de la proteína no degradable en rumen fueron: 52,51%, 56,14% para las praderas sin fertilizar con edad de corte de 30 y 60 días respectivamente y 43,38%, 45,40% para las praderas fertilizadas con edad de corte de 30 y 60 días respectivamente.With the purpose of evaluating the effect of the nitrogen fertilization and cut age on the In vitro intestinal digestibility of kikuyo grass (Pennisetum clandestinum Hochst protein, 16 plots were made, everyone assigned one of the following treatments (four plots per treatment: T1 (30 cut -day and 0 kg/ N/ Ha/ cut, T2 (60 cut-day and 0 kg/ N/ Ha/ cut, T3 (60 cut-day and 50 kg/ N/ Ha/ cut and T4 (30 cut-day and 50kg/ N/ Ha/ cut. 120 days after the treatment, 5 sub-samples were harvested of each plot, which were defined as final samples for each plot and, were realized to these samples a ruminal degradability trial for 16 hours and In vitro three-step method of

  17. Hacha y pastos: caminos de “civilización” en el Sur Occidente de Cundinamarca

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    Fabiola Estrada Herrera

    2010-07-01

    Full Text Available La autora interpreta el papel de la construcción de caminos y la exploración agrícola-comercial en el Suroccidente del actual Departamento de Cundinamarca durante la segunda mitad del siglo XIX. En este recorrido, llama la atención sobre cómo el ideario republicano de civilización conllevó a una tala irracional del bosque primario y a la introducción de nuevas especies de pastos, dos agentes de transformación que estuvieron al servicio de los ritmos que la élite decimonónica impuso a la economía local, la posesión de tierras, el control social y los proyectos agroexportadores.

  18. Crianza de hembras de reemplazo del genotipo 5/8 Holstein por 3/8Cebú en un sistema de asociación de pastos con árboles Raising replacement heifers of the 5/8 Holstein x 3/8 Zebu genotype in an associated system of pastures and trees

    Directory of Open Access Journals (Sweden)

    J.M Iglesias

    2009-03-01

    Full Text Available Se evaluó, en condiciones de producción, la potencialidad de un sistema asociado gramíneas-leucaena para la cría de hembras mestizas 5/8 H x 3/8 C, desde los 12 meses de edad hasta la incorporación a la reproducción. Se midieron las ganancias de peso vivo y el rendimiento y la calidad del pastizal y de las arbóreas. El pastoreo se realizó con 36 animales, en un área de la vaquería de la EEPF ¨Indio Hatuey¨(12 ha. La leucaena y los pastos se establecieron en años anteriores. La densidad de la arbórea fue de 555 árboles/ha y la poda en el período seco se efectuó a una altura de 150 cm. Se empleó una carga global de 3 animales/ha; la rotación fue de 50 días en la época poco lluviosa y de 40 días en la lluviosa. Los animales dispusieron de agua y sales minerales durante las 24 horas del día. No se ofreció suplementación energética ni proteica. La oferta diaria de pastos en el período seco estuvo alrededor de los 11 kg de MS/100 kg de PV/día al inicio y fue superior a los 7 kg al final; por concepto de poda y ramoneo de arbóreas los valores oscilaron entre los 0,295 y 0,133 kg de MS/100 kg de PV/día. En el período lluvioso la oferta de pastos fue de 16,85 kg de MS/100 kg de PV/día; mientras que la disponibilidad de leucaena en ramoneo superó los 0,285 kg de MS/100 kg de PV/día. La ganancia diaria de peso vivo promedio fue de 455 g, que permitió un peso de incorporación a la reproducción de 286 kg, a los 24 meses. El intervalo incorporación-cubrición promedio fue de 43 días. Se concluye que con este sistema silvopastoril es posible obtener ganancias adecuadas en pastoreo y pesos-edades a la incorporación aceptables para las condiciones actuales de la ganadería cubana.The potential of a grass-leucaena associated system for raising crossbred 5/8 H x 3/8 Z heifers, since 12 months of age until incorporation to reproduction, was evaluated under production conditions. The live weight gains and yield and pasture and

  19. APORTES A LA FORMULACIÓN DE UNA POLÍTICA PÚBLICA DE EMPLEO PARA PASTO

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    Eduardo Vicente Ruano Rosero

    2010-01-01

    Full Text Available Los indicadores del desempleo en Pasto han sido, históricamente, superiores al promedio nacional, situación que es motivo de preocupación para miles de personas en el Municipio. Pensar en una salida en el largo plazo, es una tarea que requiere incorporar en una Política Pública, no solo el reconocimiento a fondo de sus más destacadas características; sino también, adoptar una postura teórica que a de ser articulada a una decidida voluntad política y participación ciudadana e institucional. Este documento pretende aportar algunos de esos elementos teóricos y prácticos en el horizonte de formular una Política Pública de Empleo para Pasto.

  20. Multiplicación in vitro de segmentos nodales del clon de ñame Blanco de Guinea (Dioscorea cayenensis - D. rotundata ) en sistemas de cultivo semiautomatizado

    OpenAIRE

    Manuel Cabrera Jova; Rafael Gómez Kosky; Sergio Rodríguez Morales; Jorge López Torres; Aymé Rayas Cabrera; Milagros Basail Pérez; Arletys Santos Pino; Víctor Medero Vega; Germán Rodríguez Rodríguez

    2009-01-01

    Con el empleo del sistema de inmersión temporal fue posible incrementar la multiplicación in vitro de los segmentos nodales en el clon de ñame Blanco de Guinea. Con este tipo de sistema de cultivo se obtuvieron los más altos valores para la altura de la planta, número de entrenudos por planta, así como para la masa fresca y seca de las mismas. Las condiciones de cultivo creadas en el sistema de inmersión temporal para la multiplicación in vitro de los segmentos nodales lograron el más alto co...

  1. Producción de forraje y composición morfológica del pasto Mulato (Brachiaria híbrido 36061 sometido a diferentes regímenes de pastoreo

    Directory of Open Access Journals (Sweden)

    Aldenamar Cruz Hernández

    2011-01-01

    Full Text Available En Brachiaria híbrido se estudiaron tres frecuencias (14, 21 y 28 días y dos intensidades de pastoreo (9-11 y 13-15 cm, distribuidos en un diseño de bloques al azar en arreglo factorial 3 x 2 con tres repeticiones. Se midió, acumulación de forraje, tasa de crecimiento (TC composición botánica y relación hoja, tallo. No hubo efecto de int eracción (P>0.05 frecuencia x intensidad en la acumulación de forraje. La distribución del rendimiento fue de 55, 28 y 17 % durante las épocas de lluvias, nortes y seca, en el año 2007-2008, con la mayor acumulación anual (12,310 kg MS ha-1 al pastorear cada 28 días, con un incremento progresivo conforme aumentó el intervalo de pastoreo de 14 a 28 días (P<0.05. Similar comportamiento se observó para el periodo 2008-2009, la mayor TC se obtuvo en la época de lluvias con 36, 44, 47 kg MS ha-1 d-1, a frecuencias de 14, 21 y 28 días. La menor TC se presentó en la época seca. En la composición morfológica la mayor acumulación de hoja se presentó en la época de lluvias, seguido por las épocas de nortes y seca cada 28 días, a intensidad de 13-15 cm. En conclusión, la mayor producción de forraje, tasa de crecimiento, composición morfológica y relación hoja, tallo se concentró en la época de lluvias con intensidad de pastoreo de 13-15 cm cada 21 y 28 días.

  2. Aplicabilidad del test rápido y de las pruebas serológicas en el control del progreso de la oncocercosis y la filariasis linfática en la región continental de Guinea Ecuatorial

    OpenAIRE

    García Fernández, María Belén

    2018-01-01

    [EN] La oncocercosis y la filariasis linfática están catalogadas por la Organización Mundial de la Salud (OMS) como Enfermedades Tropicales Desatendidas (ETD) puesto que están asociadas a entornos de pobreza y causan discapacidad y deficiencias para toda la vida. Estas enfermedades son endémicas en Guinea Ecuatorial. En 1987 el Programa Nacional de Oncocercosis y otras Filariasis del Ministerio de Sanidad inició las actividades de control. A lo largo de estos años se realizó tratamiento...

  3. Índice de vulnerabilidad de adultos mayores en Medellín, Barranquilla y Pasto.

    Science.gov (United States)

    Cardona, Doris; Segura, Ángela; Segura, Alejandra; Muñoz, Diana; Jaramillo, Daniel; Lizcano, Douglas; Agudelo, Maite Catalina; Arango, Catalina; Morales, Santiago

    2018-05-01

    Introducción. La vulnerabilidad puede entenderse como la carencia de recursos materiales e inmateriales que impide el aprovechamiento de oportunidades en distintos aspectos de la vida. Estos recursos de bienestar evitan el deterioro de la calidad de vida.Objetivo. Construir un índice de vulnerabilidad con las características de los capitales físico, humano, social y funcional de los adultos mayores de tres ciudades de Colombia en el 2016, y determinar los factores asociados con esta condición.Materiales y métodos. Se hizo un estudio transversal con información primaria mediante 1.514 encuestas a personas de 60 años o más de Medellín, Barranquilla y Pasto. En la construcción del índice se usó el análisis factorial con los métodos de componentes principales y de rotación ortogonal varimax.Resultados. Las condiciones que generaban vulnerabilidad se relacionaron principalmente con el capital humano (calidad de vida, salud mental y hábitos); los demás capitales aportaron un solo componente, así: capital físico (ocupación), capital social (acompañamiento) y capital funcional (independencia funcional). La vulnerabilidad fue mayor en los residentes de Pasto. Los factores asociados con la vulnerabilidad fueron la ciudad de residencia, el sexo, el nivel educativo y el rol en el hogar.Conclusión. En el 58,55 % de las personas mayores, la vulnerabilidad se explicó por el uso del tiempo, la independencia funcional y el bienestar subjetivo. Estos hallazgos aportan elementos para el mejoramiento de la calidad de vida, principalmente en cuanto a la capacidad funcional para mantener la independencia, estar ocupados y fortalecer la salud mental.

  4. Morphogenic and structural characteristics of guinea grass pastures submitted to three frequencies and two defoliation severities Características morfogênicas e estruturais de pastos de capim-tanzânia submetidos a três frequências e duas severidades de desfolhação

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    Rodrigo Amorim Barbosa

    2011-05-01

    Full Text Available It was evaluated the morphogenic and structural characteristics of guinea grass under rotational at three grazing intervals and two defoliation intensities. Grazing intervals corresponded to the time needed by the forage canopy to reach 90, 95 or 100% of incident light interception during regrowth and they were evaluated combined to two defoliation severities (post-grazing conditions, 25 and 50 cm of height, being allocated to experimental units according to a complete randomized design, with three replicates and 3 × 2 factorial arrangement. The experiment was conducted from July 2003 to May 2004. For evaluation of morphogenetic and structural characteristics, ten tillers per experimental unit were selected. Morphogenetic and structural characteristics were strongly influenced by seasons of the year inasmuch as leaf elongation rate increased 3.5 fold from winter to summer. In addition to year season effect, there was also an effect of defoliation frequencies on tiller population density, which was greater in the defoliation period corresponding to 90% of light interception, especially if evaluated in relation to the interval corresponding to 100% of light interception. Defoliation frequency is determinant in expression of phenotypic plasticit, acting on the control of stem elongation.Foram avaliadas as características morfogênicas e estruturais do capim-tanzânia sob lotação rotativa em três intervalos de pastejo e duas severidades de desfolhação. Os intervalos de pastejo corresponderam aos tempos necessários para que o dossel forrageiro atingisse 90, 95 ou 100% de interceptação da luz incidente, durante a rebrotação e foram avaliados em combinação a duas severidades de desfolhação (condições pós-pastejo, 25 e 50 cm de altura, sendo alocados às unidades experimentais segundo um delineamento de blocos completos casualizados, com três repetições e arranjo fatorial 3 × 2. O experimento foi realizado de julho de 2003 a maio

  5. Una aportación al estudio del cuento fang de Guinea Ecuatorial en lengua española / A CONTRIBUTION TO THE STUDY ABOUT THE FANG TALES FROM EQUATORIAL GUINEA IN SPANISH LANGUAGE

    Directory of Open Access Journals (Sweden)

    María Teresa Abaga Envó

    2009-06-01

    Full Text Available Resumen: Una lectura sobre los estudios narratológicos revela que el acto narrativo es un hecho consustancial al ser humano. “Somos el homo sapiens, porque somos el homo narrans”, diría el académico José María Merino (2002: 57. De las estructuras narrativas, el cuento oral tradicional se alza como la forma más antigua. Tan antigua y presente en todos los pueblos que componentes semánticos y formales se repiten en relatos de distintas culturas. Tales aspectos constituyen el objeto de análisis de este repertorio de cuentos fantásticos de Guinea Ecuatorial transmitidos en lengua española. En dicho entorno, el cuento oral fang se manifiesta como un arte viviente. No es un evento pensado sólo para niños; toda la comunidad participa de esta “fiesta” que se verá enaltecida por la presencia del canto. Dada la escasa proliferación de estudios del relato oral africano y en particular del guineano, se hace imperativo partir de estudios occidentales, lo cual nos desliza hacia el terreno del comparativismo. Sin embargo, no siempre dichos estudios son confirmados en esta investigación que concluye con las siguientes tesis: el criterio verdad y ficción al que se recurre para delimitar ciertas narraciones tradicionales europeas queda difuminado; uso irregular de las fórmulas de abertura y cierre; la asignación del fin lúdico de los cuentos fantásticos se acompaña del didáctico y la pretendida universalización del método estructuralista formal de Vladimir Propp, expuesta en Morfología del cuento (1987, se vislumbra inoperante, pues quedan reducidas las treinta y una funciones y la sucesión lineal de las mismas. Los temas y los motivos, arropados por el espíritu de la cultura donde emergen, son similares a los identificados en compilaciones españolas o internacionales. Finalmente, la naturaleza oral tradicional del repertorio queda patente a través de las nueve “Leyes Épicas” establecidas por Axel Orlik (1965: la Ley de

  6. DINÁMICA DE LA PRODUCCIÓN DE PASTO Y BALANCE FORRAJERO DE UN MÓDULO DE VACAS LECHERAS, EN LA SIERRA CENTRAL

    OpenAIRE

    Avalos, Percy; Universidad Nacional Agraria La Molina, Lima, Perú.; Flores, Enrique; Universidad Nacional Agraria La Molina, Lima, Perú.

    2015-01-01

    Se condujo un experimento para estudiar la dinámica forrajera, y el contenido de proteínas y energía de una pastura con el propósito de conocer el balance forrajero para un módulo de vacunos lecheros pastoreando una asociación de ryegras-trébol. El área de estudio estuvo localizada a 4200 msnm en Conocancha, SAIS Pachacutec, Junín. La tasa de crecimiento del pasto, y la temperatura y humedad del suelo fueron medidas mensualmente usando jaulas, lapiacos, cuadrantes y termómetros medio-ambienta...

  7. La piratería de Nigeria como una amenaza a la seguridad marítima del Golfo de Guinea (2007-2012). Una aproximación desde el crimen organizado

    OpenAIRE

    Valderrama Luna, Lina María

    2015-01-01

    Desde finales de los 70 la piratería se convirtió en un problema para Nigeria. Con el tiempo adquirió características del crimen organizado y en 2010 se transformó en un problema transnacional, afectando a Estados del golfo de Guinea. Así, a través de una base conceptual, el estudio de caso concluyó que los factores internos –como la pobreza, debilidad estatal y marginalización- y externos –siendo ellos la falta de políticas internas destinadas a las aguas y la ausencia de estrategias marítim...

  8. Equatorial Guinea.

    Science.gov (United States)

    1989-03-01

    Equatorial Guinea is situated on the Gulf of Guinea along the west African coast between Cameroon and Gabon. The people are predominantly of Bantu origin. The country's ties with Spain are significant; in 1959, it became the Spanish Equatorial region ruled by Spain's commissioner general. Recent political developments in Equatorial Guinea include the formation of the Democratic Party for Equatorial Guinea in July of 1987 and the formation of a 60-member unicameral Chamber of Representatives of the People in 1983. Concerning the population, 83% of the people are Catholic and the official language is Spanish. Poverty and serious health, education and sanitary problems exist. There is no adequate hospital and few trained physicians, no dentists, and no opticians. Malaria is endemic and immunization for yellow fever is required for entrance into the country. The water is not potable and many visitors to the country bring bottled water. The tropical climate of Equatorial Guinea provides the climate for the country's largest exports and source of economy; cacao, wood and coffee. Although the country, as a whole, has progressed towards developing a participatory political system, there are still problems of governmental corruption in the face of grave health and welfare conditions. In recent years, the country has received assistance from the World Bank and the United States to aid in its development.

  9. Producción de biomasa y costos de producción de pastos estrella africana (Cynodon nlemfuensis, kikuyo (Kikuyuocloa clandestina y ryegrass perenne (Lolium perenne en lecherías de Costa Rica

    Directory of Open Access Journals (Sweden)

    Luis Villalobos

    2013-01-01

    Full Text Available Se evaluó la producción de biomasa, los costos de producción y el costo del kilogramo de materia seca en los pastos kikuyo (Kikuyuocloa clandestina, ryegrass perenne (Lolium perenne y estrella africana (Cynodon nlemfuensis, a lo largo de un año, en 8 fincas comerciales ubicadas en las provincias de Cartago (2, San José (2 y Alajuela (4. La producción de biomasa promedio por ciclo para los 3 pastos fue de 3395 kg.ha-1 MS; la producción anual se ve influenciada por los días de recuperación de cada especie, mostrando valores de 40 170, 38 731 y 28 995 kg.ha-1 de MS para los pastos estrella africana, kikuyo y ryegrass perenne, respectivamente. La producción de biomasa varía durante el año y en las épocas de mayor producción de esa biomasa, los animales tienen un menor aprovechamiento de la pastura en términos porcentuales, debido a que la carga animal, los períodos de permanencia y las áreas de pastoreo no se ajustan a la disponibilidad de forraje. Los costos anuales de mano de obra, insumos y tierra promedio fueron de 72.433, 505.515 y 18.760 colones.ha-1, respectiva- mente; siendo los insumos el rubro con un peso relativo mayor en la estructura de costos de las fincas en pastoreo. Los costos del kg de MS producido y consumido, para los 3 pastos evaluados, fueron de 16,6 y 44,4 colones respectivamente, siendo el aprovechamiento que los animales hacen de las pasturas el determinante del costo del material consumido. Las fincas con mayor inversión anual en pasturas, mostraron un mayor retorno en kg.ha-1 leche.

  10. Estudio retrospectivo de causas de trauma maxilofacial en Pasto, Colombia de 2001 a 2006 A retrospective study of maxillofacial trauma causes in Pasto, Colombia from 2001 to 2006

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    Ana Cristina Mafla

    2009-08-01

    Full Text Available Introducción: Las lesiones maxilofaciales son una condición frecuente en diferentes países, sin embargo, están asociadas a diversas causas. Metodología: Un estudio descriptivo fue realizado de Enero de 2001 a Diciembre de 2006 en tres hospitales y una institución de Pasto, Nariño, Colombia. 701 pacientes consecutivos del "Hospital San Pedro", "Hospital Universitario Departamental de Nariño", "Hospital Infantil Los Ángeles", y el Instituto de Medicina Legal y Ciencias Forenses Seccional Nariño - Putumayo fueron evaluados. Variables como año, género, distribución anatómica de la fractura, se incluyeron. Análisis descriptivos fueron usados para determinar porcentajes e intervalos de confianza. Resultados: Las causas más comunes fueron violencia, con 350 (49,9%, accidentes en motos con 104 (14,8%, en autos con 93 (13,3 %, y caídas con 66 (9,4%. Existió un incremento substancial de violencia en los años 2003 a 2004. Los niños de 0 a 6 años presentaron más caídas, mientras los jóvenes de 15 a 24 años se asociaron con violencia, al igual que accidentes en motos y automóviles. La violencia fue relacionada con fracturas de huesos propios de la nariz, caídas con lesiones naso-orbito-etmoidales, y accidentes en motos y automóviles con politraumatismo y fracturas panfaciales. Conclusiones: Debido a que la violencia, accidentes en motos y automóviles son las principales causas de lesiones maxilofaciales en la juventud, las políticas públicas deben ser dirigidas a ésta Salud UIS 2009; 41: 142-148Introduction: Maxillofacial injuries are a frequent condition in different countries; however, they are associated to different causes. Methodology: A descriptive study was performed from January 2001 to December 2006 at three hospitals and one institution from Pasto, Nariño, Colombia. 701 consecutive patients at "Hospital Los Angeles", "Hospital San Pedro", "Hospital Universitario Departamental de Nariño" and "Instituto de Medicina

  11. Temperaturas cardinales de desarrollo en la etapa siembra- emergencia de 11 pastos forrajeros

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    Noé Durán Puga

    2011-01-01

    Full Text Available Los objetivos del presente estudio fueron determinar las temperaturas cardinales de desarrollo (temperatura umbral mínima Tb, temperatura óptima To y temperatura umbral máxima Tu, e identificar un método que estime con precisión los requerimientos térmicos para la etapa siembra-emergencia (E de 11 pastos forrajeros. Ciento veinte (120 semillas de cada uno se sembraron en recipientes en condiciones controladas con un diseño experimental completamente al azar y tres repeticiones a temperaturas constantes de 15 hasta 46 ºC, en cámaras de ambiente controlado del Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP en Guadalajara, Jalisco, México. Las temperaturas cardinales y requerimiento térmico se estimaron mediante el método bilineal (MB y el método curvilíneo (MC, se evaluó su ajuste mediante comparación directa con los valores de desarrollo observados en ambiente controlado y coeficiente de variación de la duración de E expresada en términos de unidades calor acumuladas (UCA. Los resultados mostraron que el MB fue mejor para estimar Tb y To, y el MC fue mejor para estimar Tu. Los valores de Tb, To, Tu y UCA, fueron, Lolium perenne 9, 31, 41, 80, Festuca arundinacea 10, 31, 41, 78, Hyparrhenia rufa 12, 32, 42, 62, Eragrostis curvula 13, 32, 47, 40, Chloris gayana 13, 31, 47, 40, Melinis minutiflora 13, 31, 43, 50, Pennisetum clandestinum 12, 32, 43, 65, Brachiaria mutica 14, 32, 43, 57, Andropogon gayanus 12, 37, 48, 66, Cynodon dactylon 14, 29, 44, 48, Pennicetum ciliare 13, 30, 42, 61.

  12. Consumo de sustancias psicoactivas en una universidad privada de Pasto, Colombia/ Psychoactive substances at a private university from Pasto, Colombia

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    Edwin Giovanny Córdoba-Paz

    2017-06-01

    Full Text Available Este trabajo es resultado de una investigación de corte cuantitativo y de tipo descriptivo-transversal, que buscó describir y asociar factores sociodemográficos con consumo de sustancias psicoactivas en estudiantes de una universidad privada en la ciudad de Pasto, Colombia. Para cumplir el objetivo, se diseñó y validó un cuestionario que luego se aplicó a 242 estudiantes. Los resultados mostraron que el 21,9 % ha consumido algún tipo de sustancia psicoactiva y 12,4 % reporta haberlas consumido simultáneamente con alcohol. La marihuana es la droga más consumida (11,2 %, seguida de cocaína (9,1 %. El consumo de sustancias psicoactivas se asoció significativamente con sexo, siendo los hombres quienes más consumen (p < 0,05. También se asoció con semestre y estrato socioeconómico, es decir, que el consumo de sustancias psicoactivas varía en función de estas variables. Los datos advierten que la marihuana es la principal sustancia de consumo entre universitarios, simultánea al consumo de alcohol. El impacto preventivo debe focalizarse en variables como sexo y semestre.

  13. Desplazamiento forzado y condiciones de vida de las comunidades de destino: el caso de Pasto, Nariño

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    María del Pilar Bohada R.

    2010-12-01

    Full Text Available El conflicto armado en Colombia ha dejado más de 3,3 millones de personas desplazadas que buscan refugio en las ciudades. Los estudios sobre desplazamiento en el país valoran las pérdidas ocasionadas a las víctimas, pero poco analizan sus efectos sobre las condiciones de vida de las comunidades de destino. Este estudio valora los efectos del desplazamiento sobre la población residente en zonas de estrato 1 y 2 de la ciudad de Pasto, Nariño, con técnicas de evaluación de impacto. Los resultados indican que sus efectos son mixtos: negativos en seguridad y vulnerabilidad alimentaria, y positivos en confianza e infraestructura social. Lo que indica que la población desplazada no sólo genera tensiones en las comunidades de asentamiento sino que también aporta a la solución de viejos problemas sociales como la falta de infraestructura de educación y salud.

  14. ESTIMACIÓN LINEAL DE LAS FUNCIONES DE OFERTA Y DEMANDA DE LA PAPA EN EL CIUDAD DE PASTO, 1999-2010

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    Germán Marcillo

    2012-07-01

    Full Text Available A partir de consideraciones de tipo económico y matemático se especifican dos funciones de oferta y demanda de la papa en el municipio de Pasto; utilizando series de tiempo del periodo 1999 a 2010 se describen los datos de las variables empleadas para cada función a estimar; con ayuda de programas computacionales como Excel (2007 Statgraphics Centurion y Gretl (versión libre, se realizan las estimaciones de los modelos de oferta y demanda, posteriormente se desarrollan técnicas econométricas y estadística para validarlos. Finalmente, aplicando el concepto de elasticidad se analiza el efecto de cada variable con la variable en estudio. Se observó que variables como el precio y costos de producción son, en porcentaje, las que más determinan las decisiones de los ofertantes y consumidores de papa.

  15. INFLUENCIA DE TÉCNICAS DE MEJORA DE SUELOS SOBRE LA FUNCIÓN HÍDRICA DE PASTOS NATURALES ALTOANDINOS.

    OpenAIRE

    Oscanoa, Luis; Ph.D. (c) Investigador asociado, The Mountain Institute – Laboratorio Pastizales UNALM, Lima Perú.; Flores, Enrique; Ph.D. Manejo de Pastizales, Profesor Principal EPG UNALM, Lima Perú.

    2016-01-01

    Se investigaron los pastos naturales degradados de la comunidad campesina Cordillera Blanca, Huaraz – Perú. El objetivo fue estudiar la influencia de las técnicas de mejora (T) sobre el estado ecológico (Ee), recuperación hidrológica (H) y control de la erosión (Er) del pastizal de condición muy pobre. El diseño experimental fue completamente al azar con arreglo factorial (3 técnicas x 6 épocas por parámetro de estudio). La investigación duró dos años, etapa en la cual se estudiaron el Ee (ve...

  16. Manejo de pastizales en sistema de producción ganaderos de Nueva Guinea, Costa Caribe Sur de Nicaragua

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    Carlos Álvarez Amador

    2017-08-01

    Full Text Available Esta investigación se encaminó a la caracterización del manejo de las pasturas que hacen los ganaderos de Nueva Guinea, Nicaragua. El enfoque de la investigación fue cuantitativo, tipo descriptiva, se tomó una muestra de 100 ganaderos. La información fue recolectada a través de encuestas, y por las características de algunas variables también se hizo uso de formatos de recolección y medición de datos. El procesamiento de la información se realizó con el paquete estadístico SPSS versión 15,0. Se encontró una diversidad representativa de especies de pastos, tanto de pastoreo continuo como de corte, con una predominancia de retana (Ischaemum ciliare y Brachiaria brizantha cv. Marandú, estos en su mayoría con un manejo poco tecnificado, pues los productores (mujeres y hombres aún no son conscientes que las áreas de pasturas son un cultivo y deben manejarse como tal, el período de ocupación de las pasturas promedia 7 días, con una carga animal de 2,5 cabezas de ganado/manzana y un periodo de descanso de 23 días, con niveles de degradación que van desde leve hasta severo. Abstract This research was aimed at the characterization of pasture management made by cattle farmers in Nueva Guinea, Nicaragua. The research approach was quantitative, descriptive type, a sample of 100 farmers was taken. The information was collected through surveys, and the characteristics of some variables. Data collection and measurement formats were also used. The information processing was performed with the statistical package SPSS version 15.0. A representative diversity of pasture species was found, both continuous pasture and cut, with a predominance of retana (Ischaemum ciliare and Brachiaria brizantha cv. (Women and men are still not aware that pasture areas are crop and should be managed as such, the period of occupation of the pastures averages 7 days, with an animal load of 2.5 livestock per block and a rest period of 23 days, with levels of

  17. Dosis óptima de irradiación gamma con Co60 para inducción de mutagénesis en pastos

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    Alan Álvarez-Holguín

    2017-01-01

    Full Text Available La dosis letal (DL 50 y reducción media del crecimiento (RC 50 son parámetros utilizados para seleccionar dosis de irradiación adecuadas e inducir mutaciones para realizar mejoramiento genético vegetal. El objetivo fue determinar la DL 50 y RC 50 para conocer la dosis ó p tima de irradiación c on Co 60 en semilla de pasto llorón, buffel, banderita y navajita. Se evaluaron seis dosis de irradiación con Co 60 en semilla : 100, 200, 300, 450, 600 y 900 Gray (Gy y un tratamiento control (T - 0, sin irradiación. Las variables evaluadas fueron : porcentaj e de germinación (%GE, índice de velocidad de germinación (IVG, longitud de plúmula (LP y longitud de radícula (LR. Se realizó análisis de regresión y comparación de medias con la prueba de Dunnett. Mediante la ecuación de regresión se estimó la DL 50 p ara el %GE, así como RC 50 para el resto de las variables. La DL 50 se ponderó con 55%, mientras que la RC 50 se ponderó en 15% para cada una de las tres variables restantes. En general, los valores de las variables disminuyeron (P<0.05 conforme se incremen tó la dosis de irradiación, lo cual permitió determinar la DL 50 y RC 50 para cada especie. La media ponderada se obtuvo a los 628 Gy, 712 Gy, 698 Gy y 411 Gy para pasto llorón, buffel, banderita y navajita, respectivamente. Se recomiendan las dosis plantead as en este estudio, ya que esto permitirá plantear las bases para realizar mejoramiento genético a través de mutagénesis con irradiación gamma en los pastos evaluados.

  18. Colémbolos asociados con cultivos de pastos en tres zonas de vida

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    Ospina S. Claudia Marcela

    2003-12-01

    Full Text Available

    Los colémbolos son artrópodos muy pequeños,

    sin alas, similares a los insectos y extremadamente

    abundantes en gran cantidad de hábitat, siendo

    uno de los grupos de hexápodos mejor representados

    en el mundo. Son muy importantes por su influencia

    sobre la estructura de algunos suelos, no pocos viven en

    el dosel de las selvas tropicales y algunos actúan como

    dispersores de esporas dentro de los troncos en descomposición.

    En Colombia existen muy pocos estudios de

    reconocimiento de colémbolos. En la literatura se registran

    12 especies en dos localidades cerca de Buenaventura,

    agrupadas en 5 familias; así mismo, han sido

    reconocidas en diferentes estudios otras 7 especies que

    actúan como plagas en Musáceas y en flores cultivadas.

    Con el fin de contribuir al conocimiento de las familias

    de Collembola asociadas con suelos de pastos cultivados

    en el departamento de Antioquia se hicieron muestreos

    en tres zonas de vida de Holdridge (bh-P, bh-MB

    y bs-T en pastos Guinea (

     

    Panicum maximum, Ángleton

  19. Prevalencia de diabetes mellitus en la ciudad de Pasto, Nariño Colombia

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    Miguel Angel Suárez Russi

    1993-12-01

    Full Text Available Se realizó un estudio de tipo descriptivo, transversal, en la población adulta, mayor de 20 años, hombres y mujeres, habitantes de la zona urbana de la ciudad de Pasto, localizada en la zona suroccidental de Colombia, sus características son similares en alimentación y hábitos culturales al mayor porcentaje de los habitantes de Colombia de las zonas montañosas andinas, con una altura sobre el nivel del mar de 2.650 metros y una temperatura promedio de 11 grados centígrados. De una población adulta mayor de 20 años se seleccionó una muestra aleatoria sistemática de 1.430 personas. Aceptaron participar en forma voluntaria, aprovechando la estratificación de salud que tiene este municipio, distribuidos en seis sectores, sin una delimitación clara de clases sociales. Se realizó una encuesta domiciliaria sobre antecedentes familiares de diabetes y obesidad. Además se evaluaron otras variables como peso, talla, índice de masa corporal, escolaridad, para posteriormente citarlos y realizar una prueba de tolerancia a los carbohidratos, con una carga estándar de 75 gramos de glucosa, en 720 personas. Se determinó la glicemia mediante técnica de glucosa oxidasa en un equipo semiautomático cuantificando la pre y postcarga. Se emplearon para la evaluación los criterios de la OMS y National Diabetes Data Group. Al 6,0% de la población se le detectó diabetes mellitus, distribuidos en el 3,6% para mujeres y el 2,4% para los varones, siendo mayor la proporción de diabéticos entre la cuarta y quinta década. El 54,6% de los diabéticos tenían un IMC superior a 25, mientras que el 6,8% tenían problemas de obesidad con IMC superior a 30. Hubo intolerancia a los carbohidratos en el 5,3% de la población cuando se evaluó este factor aceptando la recomendación mundial de valores de glicemia entre 7,8 mmol y 11,1 mmol de glucosa en sangre venosa. El 22,5% de los sujetos diabéticos tenía antecedente materno de diabetes y paterno del 9

  20. Composición genérica del banco de semilla del suelo en un sistema silvopastoril multiasociado (Nota técnica

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    F Reyes

    Full Text Available En la Estación Experimental de Pastos y Forrajes «Indio Hatuey» se desarrolló una investigación, con el objetivo de determinar la composición, por géneros, del banco de semilla de un suelo Ferralítico Rojo en explotación ganadera; el cual estaba insertado en un sistema silvopastoril multiasociado con más de diez años de explotación. Se empleó un diseño de bloques al azar con tres repeticiones, y los tratamientos fueron las profundidades de muestreo de 0-5 y 5-10 cm. Se trabajó en un lote de 4 ha sobre el que se trazaron tres franjas, divididas en parcelas de 8 x 10 m. Las muestras de suelo se extrajeron de un área de 1 m². Se identificaron ocho géneros de gramíneas y leguminosas; los más frecuentes fueron: Panicum (78,7 %, Leucaena (9,27 %, Neonotonia (6,03 % y Teramnus (2,35 %. Hubo diferencias significativas a favor de la profundidad de 0-5 cm, en la cual se encontró la mayor proporción de semillas. Neonotonia y Leucaena presentaron el mayor porcentaje de semillas duras, aunque todas las leguminosas mostraron una alta viabilidad. Se concluye que existió una alta reserva de semilla de guinea en el suelo del sistema multiasociado, así como diversidad de géneros de leguminosas. Además, se encontró un alto porcentaje de semilla en el estrato superior del suelo (0-5 cm, lo que puede constituir una ventaja en los procesos de renovación de los pastizales con estas plantas.

  1. Una metodología muestral sugerida para la estimación de la población de la chinche de los pastos en la sabana de Bogotá

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    Galindo Julio Ricardo

    2001-12-01

    Full Text Available La chinche de los pastos (Collaria scenica Stal es una plaga
    del pasto kikuyo (Pennisetum clandestinum que causa alrededor de un 25% de pérdidas en el ingreso de los ganaderos en la Sabana de Bogotá. La evaluación de las poblaciones del insecto y su dinámica es esencial para el desarrollo de investigación en métodos de manejo del problema. En este trabajo se seleccionaron dos lotes de kikuyo de aproximadamente 1 ha, para determinar
    una metodología eficiente, económica y precisa de estimación
    de la población de estados inmaduros y adultos del insecto,
    utilizando la técnica deljameo. Se tomaron 39 muestras aproximadamente por lote y se hicieron visitas cada dos semanas, para un total de cinco. Con esta información se determinó el patrón de disposición espacial de la plaga y se definieron tamaños de muestra para niveles de precisión determinados. Además se propuso un tamaño del lote con fines de realización de pruebas experimentales, de 1/3 de ha. Finalmente se determinó la conveniencia de hacer paso y repaso sobre el recorrido de la unidad de muestreo, con el fin de lograr una mejor aproximación a la población total del insecto en el lote.

  2. Familia y Escuela, juntas construyendo convivencia y paz. Evaluación de una experiencia de intervención en Pasto, Colombia.

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Contreras Grijalba

    2017-10-01

    Full Text Available La investigación tuvo como objetivo describir el impacto de la réplica del programa FAST (Familia y escuelas juntas desarrollado con estudiantes, docentes y padres de familia del Jardín Infantil Piloto de la ciudad de Pasto (Colombia. Método: La reconstrucción de la experiencia vivida se realizó desde el enfoque cualitativo constructivista, utilizando un método histórico hermenéutico a través de entrevistas en profundidad. Resultados: Mejoró el funcionamiento familiar, se fortaleció la resiliencia infantil y se mitigaron los factores de riesgo desde los aportes de las teorías ecológica, sistémica y del desarrollo emocional de los niños y niñas. Conclusión: Se evidencia la gestación de una nueva convivencia desde la escuela para la familia.

  3. CARTOGRAFÍA DE LA VEGETACIÓN DE LA ISLA DE BIOKO (GUINEA ECUATORIAL MEDIANTE EL USO DE IMÁGENES LANDSAT 7 ETM+: PARTICULARIZACIÓN DEL PISO AFROMONTANO

    Directory of Open Access Journals (Sweden)

    Rafael M. Navarro Cerrillo

    2012-01-01

    Full Text Available La gestión forestal sostenible requiere de una información cartográfica adecuada. En este trabajo se elaboró una cartografía actual de la vegetación de la isla de Bioko (Guinea Ecuatorial basada en la clasificación supervisada de una imagen Landsat 7 ETM+, y una cartografía de la vegetación del piso afromontano, que incluye datos de la distribución, la superficie y los tipos forestales con presencia de Prunus africana. Se proponen seis nuevos tipos de vegetación de transición entre los bosques Guineo- Congolesa y los bosques afromontanos, y distintas formaciones afromontanas secundarias. El mapa de vegetación de Bioko tuvo un coeficiente Kappa de 0,69. Los principales errores se asociaron a las clases correspondientes a bosque secundarios de tierras bajas y a las zonas de transición entre los bosques congoleses y afromontanos. La fiabilidad del análisis mejoró en una segunda clasificación restringida a la vegetación afromontana de la isla (> 1400 m s.m., obteniéndose un coeficiente Kappa de 0,81. En este trabajo se muestra la utilidad de la teledetección para la elaboración de cartografía en zonas tropicales de escasa accesibilidad, dado que se obtuvieron resultados con una fiabilidad adecuada para la gestión forestal.

  4. Estructura de un ecotono bosque Subalpino-pastos alpinos (Las Cutas, Ordesa, Pirineos Centrales

    Directory of Open Access Journals (Sweden)

    Camarero, J. J.

    1999-12-01

    Full Text Available We describe the structure of a subalpine forest-alpine pasture ecotone in the Central Pyrenees, that includes altitudinal timberline and treeline, and it is dominated by Pinus uncinata Ram. We measured for each P. uncinata 21individual located inside a rectangular plot parallel to the slope: the location and several structural and groivth form variables (number and type of stems per individual. Then, P. uncinata individuals were classified according to their size (adults, poles, saplings and seedlings and growth form (krummholz —shrubby and multistemmed individuals—, krummholz with vertical stems. The ecotone structure was described through the study of groivth form and size changes of P. uncinata individuals across the ecotone and related to the area climate, that is characterized by maximum snow thickness in spring (April and strong N-NW-W winds. Most of living individuals were krummholz, located above the timberline in the area where seedlings were abundant. Bigger, vertical and unistemmed individuals predominated in the forest. The structure or growth form changes were sharp across the ecotone. Crown injuries due to wind effects were evident in shrubby individuals and predominated in those directions that showed stronger winds during the year. The snow-wind interaction can explain partially the location of the studied timberline that could be considered a local phenomenon. The spatial location of the different classes of individuals, the spatial interaction between seedlings and krummholz individuals, and the changes of growth form (from arborescent to shrubby or vice versa can modify the response of these P. uncinata populations to climate changes.

    [es] Describimos la estructura de un ecotono bosque subalpino-pastos alpinos en los Pirineos Centrales, que incluye los límites altitudinales del bosque y del árbol y está dominado por Pinus uncinata Ram. Para cada individuo de

  5. Estrategias para establecer pastos de temporal en zonas semiáridas de México.

    OpenAIRE

    Hernández Guzmán, Filogonio Jesús

    2013-01-01

    Para incrementar la probabilidad de establecimiento de pastos, en siembras de temporal en zonas semiáridas, se caracterizaron física y fisiológicamente semillas de Banderita, Navajita; Buffel y Rhodes y, en base a dimensiones y utilizando tamiz, se clasificaron por tamaño de cariópside (TC) en chicos (Cch), medianos (CM) y grandes (CG). En Banderita y Buffel, los diámetros de tamiz para CG, CM y Cch fueron de 0.70, 0.59 y 0.50 mm y para Navajita y Rhodes, 0.59, 0.50 y 0.42 mm, respectivamente...

  6. Guinea-Bissau.

    Science.gov (United States)

    1985-04-01

    This discussion of Guinea-Bissau focuses on the following: the people; geography; history; government and political conditions; the economy; foreign relations; and relations between the US and Guinea-Bissau. In 1983 the population was estimated at 827,000 with an annual estimated growth rate of 2.2%. 87% of the population was rural, with 13% living in urban areas. The infant mortality rate is 200/1000 with a life expectancy of 42 years. The population comprises several diverse tribal groups, each with its own language, customs, and social organization. Most people are farmers and follow the traditional religious beliefs of their forebears. Most of the nonindigenous population consists of a significant Cape Verdian ethnic community, a small Portuguese community engaged primarily in commerce and civil service, and a few foreign technical consultants. Guinea-Bissau is a small enclave on the west coast of Africa, bounded by Senegal and on the south and east by Guinea (Conakry). Portugal claimed Portuguese Guinea in 1446 when the fleet of Nuno Tristao arrived on the west coast of Africa. In 1956 the African Party for the Independence of Guinea and Cape Verde (PAIGC) was organized clandestinely by the Amilcar Cabral and Raphael Barbosa. The PAIGC moved its headquarters to Conakry in 1960 and began an armed rebellion in 1961. Despite the presence of more than 30,000 Portuguese troops, the PAIGC exercised influence over much of the country by 1972. It established civilian rule in the territory that it controlled and elections took place for a National Assembly. The National Assembly of the PAIGC declared the independence of Guinea-Bissau on September 24, 1973. Portugal granted "de jure" independence on September 10, 1974, when the US recognized the new nation. Guinea-Bissau is a 1-party state. Until May 1984, power was held by a provisional government responsible to a Revolutionary Council. In May 1984 the Revolutionary Council was dissolved, and the 150-member National

  7. Contenido nutricional, digestibilidad y rendimiento de biomasa de pastos nativos que predominan en las cuencas ganaderas de Molinopampa, Pomacochas y Leymebamba, Amazonas, Perú

    Directory of Open Access Journals (Sweden)

    Manuel Oliva

    2015-01-01

    Full Text Available El objetivo del presente trabajo de investigación consistió en analizar el contenido nutricional, digestibilidad in vitro y el rendimiento de biomasa de las especies y según los ganaderos son las especies de pastos nativos más importantes que predominan en las tres principales cuencas ganaderas del departamento de Amazonas: Molinopampa, Pomacochas y Leymebamba. Se muestrearon al azar, seis áreas de 0,25 m2 por tratamiento cada 60 días durante tres brotes. Las muestras obtenidas fueron mezcladas y pesadas por separado, para luego extraer una alícuota homogénea y representativa de 1 kg por especie, para ser deshidrato en una estufa a 60 °C por 48 horas, las mismas fueron sometidas a un análisis proximal. Con los valores obtenidos de cada uno de los parámetros de evaluación y luego de ser multiplicados con sus respectivos valores ponderados se calculó el valor acumulativo final siendo los resultados las siguientes Trifolium repens L (35,9, Philoglossa mimuloides-Siso menudo (35,3, Cenchrus clandestinus (32,8, Trifolium dubium (32,5 y Philoglossa mimuloides-Siso lapacho (31,3. Los valores significan la importancia acumulativa de cada especie.

  8. Vacuna fenol-insoluble contra la brucelosis humana: evaluacion del poder inmunogenico en cobayos Phenol insoluble extract vaccine for the prevention of brucellosis in humans: evaluation in guinea pigs

    Directory of Open Access Journals (Sweden)

    J. Bolpe

    1991-02-01

    Full Text Available Se examinó una vacuna diseñada para inmunizar al hombre, preparada con extracto de fenol insoluble, para determinar si protegía a cobayos contra el desafío con la cepa virulenta B. abortus 2308. Se incluyeron en el experimento las vacunas vivas atenuadas B. abortus cepa 19 y B. melitensis Rev. 1, para comparar los resultados. Se vacunaron 93 animales en cada grupo, que fueron subdivididos en subgrupos de 31 y se los desafió con 10(4, 10³ y 10² unidades formadoras de colonias de la cepa B. abortus 2308 virulenta. El análisis global de los resultados demostró una protección del 11.9% en animales vacunados con el extracto de fenol insoluble, 65% en los vacunados con B. abortus cepa 19 y 95% en el grupo que recibió vacuna B. melitensis Rev. 1.A phenol insoluble extract vaccine proposed to immunize men against brucellosis was tested for its ability in protecting guinea pigs against challenge with virulent Brucella abortus strain 2308. Living attenuated Brucella abortus strain 19 and B. melitensis Rev. 1 were included in the experiment for comparison. Ninety three animals were vaccinated in each group and subdivided in subgroups of 31 for challenge with 10(4,10³ and 10² colony forming units of virulent B. abortus 2308. A global analysis of the results showed protection of 11.9%, 65% and 95% in animals vaccinated with phenol insoluble extract, strain 19 and Rev. 1, respectively.

  9. Demographic and clinical determinants associated with intrauterine fetal mortality in the municipality of Pasto, 2010 - 2012

    Directory of Open Access Journals (Sweden)

    Daniel Jurado Fajardo

    2017-08-01

    Full Text Available Introduction: Intrauterine fetal mortality (IUFM is a public health problem because of its high rates worldwide and in low-and middle-income populations. However, it is a little-studied event and lacks visibility in public health policies, plans and programs. Objective: To analyze the sociodemographic and clinical determinants associated with IUFM in Pasto-Colombia. Materials and methods: A study, that includes 88 fetal deaths as cases and 88 live births as controls occurred in third level hospitals in Pasto-Colombia during 2010 and 2012, was carried out to determine the relationship between fetal mortality, clinical conditions (complications of pregnancy, gestational age, birth weight, prenatal controls, pathological and toxic medical history, or abortion and sociodemographic conditions (age, ethnicity, occupation, marital status, stratum, area of residence , schooling, parity, displacement condition, planned pregnancy. Results: It was identified that the risk of fetal mortality is significantly lower with the increase in gestational age (OR ajustado = 0.76 IC95% 0.62; 0.93 and birth weight (OR ajustado = 0.99 IC95% 0.98; 0.99. Other clinical and sociodemographic variables were not associated. Conclusion: The results provide evidence for planning intervention plans that prioritize women whose fetus has a lower-than-normal weight and a risk of premature birth.

  10. Desarrollo y validación de un método ambientalmente amigable para determinación de metales pesados en pastos

    Directory of Open Access Journals (Sweden)

    Amanda Lucia Chaparro

    2016-12-01

    Full Text Available Los metales pesados son peligrosos para los seres vivos y el medio ambiente, pues no pueden ser degradados y pueden llegar a los seres humanos a través la cadena trófica, por lo tanto, su determinación en diferentes matrices para consumo humano y animal, es de vital importancia. La metodología más utilizada para su extracción, es cenizas, la cual conlleva a tiempos largos de análisis y elevado consumo de energía, por esta razón, en el presente trabajo se desarrolló y validó un método ambientalmente amigable para la digestión y extracción de Cd+2 y Pb+2 en pastos, utilizando ultrasonido y espectroscopia de absorción atómica. Se evaluaron diferentes variables que afectan la digestión, como posición en el ultrasonido, peso de muestra, solvente, volumen del solvente, tiempo de sonicación y tipo de recipiente, en muestras de pastos dopadas con los metales, teniéndose como variable de respuesta, el porcentaje de recuperación. Las condiciones para extraer Cd+2 y Pb+2 en estas pasturas, fueron: 0,50g de tejido vegetal; 3,00mL de HCl:HNO3-3:1 expuestos 60min al ultrasonido, en tubos de vidrio cónicos. Este procedimiento mostró un comportamiento lineal en el rango de 0,50 - 8,00mg/Kg con R2 de 0,99 tanto para Cd como para Pb. Los límites de detección y cuantificación, fueron 0,25 y 0,43mg/Kg para Cd; 0,22 y 0,37mg/Kg para Pb. La metodología propuesta, se comparó con la técnica clásica y permitió determinar niveles trazas de estos metales tóxicos, en forraje proveniente del Magdalena medio, con porcentajes de recuperación de 97,54 y 98,62%, respectivamente.

  11. Das sesmarias à resistência ao cercamento: razões históricas dos Fundos de Pasto

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Ferraro Júnior

    Full Text Available O artigo recupera as razões históricas para o surgimento da categoria dos fundos de pasto. Nele, são utilizados dados secundários recolhidos de relatórios governamentais e obtidos em entrevistas com técnicos, agentes pastorais e comunidades pastoris. Os fundos de pasto eram apenas áreas não-cercadas de Caatinga, utilizadas para pastoreio comunal. Esse padrão de ocupação, que se desenvolveu em todo o semiárido nordestino, foi progressivamente usurpado em um processo similar aos enclosures ingleses. Na Bahia, com os avanços do capital sobre essas áreas, a partir da década de 1970, houve articulações regionais e apoios institucionais que estimularam resistências diversas. Assim, "Fundo de Pasto" passou a designar não só as áreas, mas os grupos sociais que as defendiam por delas depender. O termo Fundo de Pasto, antes regional, generalizou-se por todo o estado, principalmente após sua citação na constituição baiana. Os vínculos familiares dessas comunidades também concorreram para a resistência dessa forma de ocupação.

  12. Estudio de las conductas prosociales en niños de San Juan de Pasto/ Prosocial Behaviors Study in Children San Juan de Pasto

    Directory of Open Access Journals (Sweden)

    Érika Alexandra Vásquez Arteaga

    2017-06-01

    Full Text Available Objetivo: El presente artículo es el resultado de una investigación finalizada desarrollada en tres escuelas públicas de San Juan de Pasto. El objetivo general se dirigió a develar las manifestaciones de las conductas prosociales de los niños para el diseño e implementación de una estrategia psicopedagógica. Método: La metodología se abordó desde el paradigma cualitativo, con un enfoque crítico social, de tipo investigación-acción. El presente artículo corresponde a la fase hermenéutica de la investigación en donde se emplearon como técnicas de recolección de información: narrativas, entrevistas focalizadas, observaciones participantes y sociodramas. Resultados: Los resultados indicaron que la empatía puede ser vista como una señal de debilidad o como una constante necesaria de interacción; en las conductas de ayuda hay manifestaciones principalmente no altruistas y la cooperación se presenta de manera intermitente según los estímulos ambientales.

  13. Dracunculiasis (guinea worm disease).

    Science.gov (United States)

    Greenaway, Chris

    2004-02-17

    Dracunculiasis (guinea worm disease) is a parasitic disease that is limited to remote, rural villages in 13 sub-Saharan African countries that do not have access to safe drinking water. It is one the next diseases targeted for eradication by the World Health Organization. Guinea worm disease is transmitted by drinking water containing copepods (water fleas) that are infected with Dracunculiasis medinensis larvae. One year after human ingestion of infected water a female adult worm emerges, typically from a lower extremity, producing painful ulcers that can impair mobility for up to several weeks. This disease occurs annually when agricultural activities are at their peak. Large proportions of economically productive individuals of a village are usually affected simultaneously, resulting in decreased agricultural productivity and economic hardship. Eradication of guinea worm disease depends on prevention, as there is no effective treatment or vaccine. Since 1986, there has been a 98% reduction in guinea worm disease worldwide, achieved primarily through community-based programs. These programs have educated local populations on how to filter drinking water to remove the parasite and how to prevent those with ulcers from infecting drinking-water sources. Complete eradication will require sustained high-level political, financial and community support.

  14. Guinea pig maximization test

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner

    1985-01-01

    Guinea pig maximization tests (GPMT) with chlorocresol were performed to ascertain whether the sensitization rate was affected by minor changes in the Freund's complete adjuvant (FCA) emulsion used. Three types of emulsion were evaluated: the oil phase was mixed with propylene glycol, saline...

  15. Respuesta de Physalis peruviana a la fertilización con diferentes dosis de N, P y K en el Altiplano de Pasto, Colombia

    Directory of Open Access Journals (Sweden)

    Amanda Silva Parra

    2015-10-01

    Full Text Available En un Andisol Typic dystrandept del Altiplano de Pasto (2800 m.s.n.m., 12.6°C, 820 mm/año y 900 h de luz solar, Colombia, se evaluaron algunas variables de crecimiento, contenido de nutrientes y producción de uvilla (Physalis peruviana como respuesta a la fertilización con N, P y K. Los tratamientos consistieron en la aplicación de urea (46% N como fuente de N, superfosfato triple-SPT (46% de P2O5 como fuente de P y cloruro de potasio-KCl (60% K2O como fuente de K, en dosis iguales para cada fuente, de 80, 150 y 300 kg/ha/año aplicados en dos épocas al momento del trasplante y 30 días después, antes de la floración. Los resultados mostraron diferencias en fuentes y dosis (P ≤ 0.01 en todas las variables analizadas. Las dosis de 300 kg/ha de urea, 150 kg/ha de SPT y 300 kg/ha de KCl favorecieron la mayor altura de planta, la materia seca y la absorción de NPK (P ≤ 0.05. La absorción de N fue mayor que la de K y éste a su vez que la de P en todas las interacciones evaluadas (P ≤ 0.05. La aplicación de 300 kg/ha de cada uno de los fertilizantes ocasiono incremento del rendimiento de P. peruviana. Por otra parte, fueron encontrados efectos positivos en la absorción NPK en relación con los rendimientos de Physalis peruviana con 300 kg/ha de urea, 150 kg/ha de SPT y 300 kg/ha de KCl (P ≤ 0.05, por tanto se recomiendan estas dosis de fertilizantes.

  16. A Diagnosis of English Language Teaching in Public Elementary Schools in Pasto, Colombia

    Directory of Open Access Journals (Sweden)

    Jesús Alirio Bastidas

    2011-12-01

    Full Text Available English teaching in Colombian primary schools became a requirement because of the promulgation of the Law of Education in 1994. Taking into account that this decision produced some difficulties in the schools, a study was conducted to diagnose the state of English language teaching in primary schools in Pasto, Colombia. Data were gathered through classroom observation, a questionnaire, and interviews. The results showed that teachers are not well versed either in methodology or in the command of the English language; there was no English syllabus; didactic materials were nonexistent; and the children’s lack of motivation was the most critical problem. Teachers, institutions and the government have to take into account these findings in order to improve English learning in primary schools.

  17. Violencia contra las mujeres en tres ciudades de Colombia: Pasto, Cartagena y Cali. 2005-2009

    Directory of Open Access Journals (Sweden)

    Beatriz Londoño Toro

    2014-05-01

    Full Text Available Este artículo presenta los resultados de una investigación que evidencia la gravedad de la situación de violencia contra la mujer con un examen específico de este fenómeno en las relaciones de pareja. El aporte de las investigadoras radica en la documentación de los principales problemas referidos a la dinámica de las vulneraciones, tipologías de agresores, formas de violencia e incluso períodos en que se incrementan estos fenómenos a partir de las cifras de los Observatorios Regionales de Cali, Pasto y Cartagena (Colombia. Finaliza con unas propuestas relacionadas con el fortalecimiento de las políticas públicas, respuesta estatal e institucional y diseño de mecanismos de acceso a la justicia para las mujeres.

  18. Estrategias para garantizar la seguridad alimentaria y nutricional de la mujer indígena de la comunidad de los pastos residentes en Bogotá.

    Directory of Open Access Journals (Sweden)

    Elizabeth Valoyes Bejarano

    2012-03-01

    Objetivo. Determinar las estrategias utilizadas en las mujeres indígenas  pertenecientes a la comunidad de los pastos y residente en Bogotá, para garantizar la seguridad alimentaria de sus familias. Materiales y métodos. Estudio de casos realizado con entrevistas semiestructuradas a 8 familias indígenas residentes en Bogotá. Resultados. Las estrategias utilizadas por las mujeres fueron: ayuda del estado por medio de comedores comunitarios y jardines infantiles, redes de apoyo que conforman las mujeres con el fin de obtener apoyo económico, moral y social, empleo, consumo insuficiente de alimentos por parte de la mujer para satisfacer las necesidades de su familia, reducción de tiempos de comida, obtener alimentos en promoción o en cosecha. Conclusiones. Este estudio confirmó que la mujer es la base de la SAN en sus familias, sin embargo algunas de las estrategias utilizadas limitan su derecho a la alimentación. Por otra parte el estudio se acercó a definir la relación entre SAN, etnia y género, la cual se ha investigado poco y posee en cada una de estas categorías un número importante de variables que dejan posibilidades para seguir investigando en la comprensión de sus relaciones.

  19. Situación laboral de la mujer profesional en Pasto, 2010: Un análisis de modelación micro-econométrica

    Directory of Open Access Journals (Sweden)

    Julio Cesar Riascos

    2012-01-01

    Full Text Available En el artículo se examina la situación laboral de la mujer profesional y su relación con la educación superior en San Juan de Pasto durante el año 2010. Para tal efecto, se describen las características generales de una profesional y se miden los principales indicadores asociados al mercado de trabajo bajo los parámetros proferidos por la metodología de la Gran Encuesta Integrada de Hogares del DANE versión 2009. En el documento también se cuantifican los retornos en educación por área de conocimiento y se identifican las principales variables que explican los niveles de ingreso y la probabilidad de empleo formal y estable de la mujer profesional, lo anterior después de inspeccionar 412 factores educativos y laborales y cerca de 172.000 correlaciones estadísticas. La investigación sugiere que los tres principales problemas que enfrenta la mujer profesional en el mercado laboral de la Ciudad son el elevado subempleo, en especial por insuficiencia de horas, el desempleo, y la discriminación salarial, factores que, a su turno, pueden reducirse con el acceso a niveles de postgrado permitiendo mejorar el nivel de ingresos y estabilidad ocupacional.

  20. AUTOPOLINIZACIÓN EN LA PRODUCCIÓN DE SEMILLA DE PASTO BUFFEL (Cenchrus ciliaris L.

    Directory of Open Access Journals (Sweden)

    Elizabeth Conde-Lozano

    2011-01-01

    Full Text Available El objetivo de este estudio fue determinar el efecto de la polinización libre y la autopolinización en el pasto Buffel. El estudio se realizó en el invernadero y en el campo de la Unidad Académica Multidisciplinaria Agronomía y Ciencias (UAMAC en el Municipio de Victoria, Tamaulipas. Se utilizaron cuatro variedades de pasto Buffel (Común, Nueces, T-1754 y Formidable las cuales fueron sometidas a dos tipos de polinización (libre y auto. Se utilizó un diseño en bloques completos al azar. Se midieron los días a floración de los órganos masculinos y femeninos. Se clasificó el polen (fértil, intermedio e infértil y los efectos de la autopolinización y polinización libre sobre las características de las semillas. No se observaron efectos sobre los días a floración de los órganos femeninos (P = 0,54, pero sí en los órganos masculinos (P < 0,03, donde las plantas de la variedad Formidable tardaron 2,3 días en madurar. El número de granos de polen fértil, intermedio e infértil no fueron afectados por la polinización ni por la variedad. En cuanto a los componentes de la semilla, todos fueron afectados (P < 0,01 por el tipo de polinización, las plantas sometidas a autopolinización mostraron las mejores características. La polinización afectó las características de la semilla.

  1. [Enteroparasite determination in Lactuca sativa from farms dedicated to its production in Pasto, Colombia].

    Science.gov (United States)

    Polo, Giovanni Andrés; Benavides, Carmenza Janneth; Astaiza, Juan Manuel; Vallejo, Dario Antonio; Betancourt, Patricia

    2016-12-01

    Currently, vegetables like lettuce are widely recommended as part of the daily diet given their high nutritional value; however, while consumers feel attracted to the benefits provided by the vegetable, they may also be exposed to parasitic intestinal infections. To determine the presence or absence of enteroparasites in lettuce (Lactuca sativa) grown in the rural area in the municipality of Pasto, and to analyze associated factors based on the characterization of the lands. We conducted a descriptive double blind cross-sectional study. We took a total of 105 samples from 21 properties from June to December, 2013, and we processed them by sedimentation and flotation tests. Additionally, the owners were surveyed in order to obtain information about the possible variables influencing the occurrence of enteroparasites. We detected contamination in 100% of the lettuce samples and we found parasite eggs and larvae as follows: 95.25% with Entamoeba spp. cysts; 71.43% with Isospora spp. oocysts; 61.90% with Strongyloides stercoralis larvae (L3); 28.57% with Toxocara spp. eggs, and 4.76% with Eimeria spp. oocysts. Using the chi-square test we found association between Entamoeba spp. and ditches (p=0.008), dogs (p=0.008) and septic tanks (p=0.029); between Isospora spp. and compost (p=0.0001), dogs (p=0.0001) and slugs (p=0.002); between S. stercoralis and handling (p=0.003), and between Toxocara spp. and no use of biodigesters (p= 0.002). We found contamination with enteroparasites in lettuce samples from growing areas in the municipality of Pasto with animal and human sources as their main reservoirs, although others were present in the environment.

  2. Avances en la evaluación de leguminosas anuales distintas del T. subterraneum L.

    OpenAIRE

    Moreno Cruz, V.; Gallardo Martínez, D.

    2011-01-01

    Dado los problemas actuales de persistencia del T. subterraneum en muchas áreas, debidas en general, al estrecho margen de adaptacóin de las variedades disponibles actualmente, se indican en el presente trabajo otras especies de leguminosas anuales que pueden ser una alternativa, en los pastos del S-O, al referido Trifolium subterraneum. Se incluye, entre las evaluaciones realizadas, las especies Medicago polymorpha, Trifolium glomeratum y Ornithopus compressus, todas ellas presentes en los s...

  3. The guinea-pig

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Maibach, H I; Anjo, M D

    1980-01-01

    14C ring-labelled hydrocortisone, testosterone and benzoic acid dissolved in acetone were applied to the backs of guinea-pigs (4 microgram/cm2). Percutaneous absorption was quantified by following the excretion of tracer in urine and faeces for 5 days. Absorption of hydrocortisone and benzoic acid...... was 2.4% (s.d. = 0.5; n = 3) and 31.4% (s.d. = 9.1; n = 3) of the applied dose respectively, similar to published human absorption data. Testosterone was absorbed to a greater extent in guinea-pigs (34.9% +/- 5.4; n = 5) than man. A thioglycollate based depilatory cream significantly increased the skin...

  4. Papua New Guinea.

    Science.gov (United States)

    1986-10-01

    The situation of the island archipelago nation of Papua New Guinea is reviewed in terms of its geography, people, history, government, politics economy and foreign relations. Papua New Guinea consists of the eastern half of the main island of New Guinea (the western portion is Indonesia), and several island groups, all located northeast of Australia. The island has extreme geographical variations, ranging from precipitous mountains to extensive swamps and river valleys, all in a monsoon cimate. As a result, there are hundreds of small groups each with unique cultures and languages; over 650 languages, many unrelated to each other. The population is estimated at 3.5 million, growing at about 2.5% per year. Historically, the region has been occupied by Germany, Britain, Japan, and the U.S. The country came under the international trusteeship system in 1949, and now has a vigorous parliamentary government. The economy is based on subsistence agriculture, but is buttressed by resources such as gold, copper, and other metals, oil, timber, tropical agricultural products, fish. The only indusry is local production, since the minimum wage is too high to compete with Asian labor.

  5. Human talent development processes in a specialty clinic in Pasto, Colombia

    Directory of Open Access Journals (Sweden)

    Jeraldine Milena Matabajoy-Montilla

    2018-01-01

    Full Text Available Introduction: Workers in the organization play a fundamental role in meeting the corporate objectives. In this sense, it is important to identify the processes of human talent development in the health sector since they contribute to human and organizational development. Objective: The processes of human talent development training, quality of work life, occupational health, organizational climate and performance evaluation were characterized in a specialty clinic in the city of Pasto, Colombia. Materials and methods: The study was addressed from the quantitative paradigm of descriptive type with a sample of 211 collaborators. The scale called "perception of human talent development processes" was built, whose Alfa de Cronbach was 0.97. Results: The results were systematized in the statistical program IBM SPSS statistics. Statistically significant differences were found in the training category (in the categories of needs detection, plan and programmes, training evaluation and follow-up, quality of working life (in the sub-categories: meaning of work and labor commitment and performance evaluation (in the subcategories: methods and results. Conclusions: The need to continue strengthening the processes of training, quality of work life and evaluation of performance is recognized.

  6. Motivaciones que impulsan al cambio de trayectoria vital en los emprendedores (Pasto, Colombia, 2012-2013

    Directory of Open Access Journals (Sweden)

    Claudia Magali Solarte Solarte

    2015-05-01

    Full Text Available Este documento es producto de una investigación, cuyo objetivo general fue analizar las motivaciones que impulsan al cambio de trayectoria vital en los emprendedores de la ciudad de Pasto. Se trabajó con creadores de microempresas y pequeñas empresas, con quienes, bajo el enfoque de investigación cuantitativo, método empírico-analítico y tipo descriptivo, a través de una encuesta, se identificaron dichas motivaciones y se determinó cuáles de ellas tuvieron mayor incidencia. Para un mejor manejo de la información, se clasificaron las motivaciones de emprendimiento según la adaptación realizada por Rodrigo Varela sobre la teoría de Albert Shapero en cuanto a la clasificación de impulsos positivos, neutros y negativos, en la cual se tienen en cuenta componentes socioculturales, educativos e investigativos que constituyen niveles de apreciación para desentrañar motivaciones particulares que hacen posible una acción empresarial, a partir de conocimientos, valores, aptitudes, actitudes y competencias.

  7. INVESTIGACIÓN DE MERCADO SOBRE EL GRADO DE ACEPTACIÓN DE LA CARNE DE CUY (CAVIA PORCELLUS EN PRESENTACIONES DE AHUMADO, CROQUETAS Y APANADO EN LA CIUDAD DE PASTO MARKET RESEARCH ON THE DEGREE OF ACCEPTANCE OF MEAT FROM CUY (CAVIA PORCELLUS PRESENTATIONS SMOKED, AND BROSTER CROQUETTES IN THE CITY OF PASTO

    Directory of Open Access Journals (Sweden)

    FRANCISCO E ARGOTE

    2009-06-01

    Full Text Available Los cambios de hábitos en el consumo de alimentos, insinúan productos de fácil preparación y oportuna adquisición en supermercados, motivo por el cual se hizo necesario indagar a los consumidores de la ciudad de Pasto, su interés y disponibilidad por adquirir Cuy, en presentaciones diferentes a la tradicional. Los objetivos desarrollados fueron valoración de la demanda potencial, realización de test de mercado, determinación de la participación de la carne de Cuy con sustitutos y diseño de estrategias de mercadeo. El modelo de investigación empleado fue el cuantitativo y obedeció a muestreo probabilístico aleatorio con estratificación proporcional. La recolección de la información se realizó mediante encuesta. La población objeto de estudio de la ciudad de Pasto correspondió a 27.428 hogares distribuidos en los estratos, tres, cuatro, cinco y seis. La muestra calculada fue de 383 observaciones, con un nivel de confianza del 95% y un margen de error del 5%. Las estrategias se formularon con base en la mezcla de mercadeo (precio, producto, plaza, promoción y publicidad. Como resultados y conclusiones se logró determinar que el consumo de la carne de Cuy es ocasional con cantidades entre una y cuatro canales. El Cuy en el momento no se vende en famas ni en supermercados, los precios se analizaron en asaderos comerciales (700 gramos de Cuy asado $24.000 y plazas de mercados (Kilo de Cuy vivo alrededor de $12.000. El porcentaje de participación de la carne de Cuy fue de 3,3% del consumo total de carnes. El estimativo de la demanda potencial para las nuevas presentaciones fue de: 92.158 unidades/año para el Cuy ahumado, 69.119 unidades/año para el apanado, y croquetas con 65.827 unidades/año, con pesos de 750 g y 500 g. Los encuestados prefirieron la carne de Cuy ahumada.The changes of habits in the consumption of foods, insinuate products of easy preparation and opportune acquisition in supermarkets, reason for which became

  8. El mejoramiento de la gestión del talento humano a través del empowerment en las micro, pequeñas y medianas empresas, transformadoras de alimentos afiliadas a ACOPI seccional Nariño

    OpenAIRE

    Mora Gómez, Nelson Armando

    2011-01-01

    Tesis de grado (Maestría en Gerencia del Talento Humano). Universidad de Manizales. Facultad de Ciencias Sociales y Humanas, 2011 Uno de los propósitos de esta investigación fue conocer la situación en materia de Gestión del Talento Humano en las pymes del sector alimentos afiliadas a ACOPI seccional Nariño, se tomo como referencica 6 empresas de la ciudad de Pasto, que estuvieron dispuestas a ser parte del estudio. Desde la Gestión del Talento Humano se tuvo en cuenta principalmente los p...

  9. Lamington, Papua New Guinea Images

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dormant stratovolcano in Papua New Guinea suddenly exploded in 1951. Nuees ardentes (glowing avalanches) shot down the mountain at 100 km per hour, devastated...

  10. Luchas de los sectores LGBTI en Pasto, Nariño en el posconflicto

    Directory of Open Access Journals (Sweden)

    Scarlet Sotomayor Tacuri

    2017-12-01

    Full Text Available La categorización de víctima constituye una estrategia de participación política de los sectores LGBTI de la ciudad de Pasto. El fin de esta autodefinición es continuar la lucha por la ciudadanía plena y su reconocimiento en el departamento de Nariño, Colombia. Este colectivo es heterogéneo y tiene dificultades a la hora de construir una identidad colectiva que permita su consolidación. Sin embargo, durante los dieciséis años que lleva funcionando se ha fortalecido, mediante las luchas constantes de activistas y las transformaciones constitucionales. Se tienen en cuenta los debates feministas y de los estudios de género sobre víctimas y ciudadanía, planteándolas como definiciones opuestas; no significa que sean en sí mismas un antagonismo, sino una estrategia de movilización política en el marco de la Ley 1448 de 2011 y en los acuerdos de paz realizados entre el gobierno nacional y las Fuerzas Armadas Revolucionarias de Colombia -FARC- durante los últimos cuatro años. Esto ha permitido la visibilización de sus problemáticas, a nivel social y cultural en las zonas rurales, donde se recrudece el conflicto y, en la zona urbana donde los estereotipos y estigmas son parte de la cotidianidad y se reproducen para discriminar a lesbianas, gays, bisexuales, trans e intersexuales.

  11. Carbonate Formation And Diagenesis In Pastos Grandes Laguna (Bolivia): Modern Analog For The South Atlantic Cretaceous Presalt Travertinoid Deposits

    Science.gov (United States)

    Muller, E.; Ader, M.; Gérard, E.; Virgone, A.; Gaucher, E.; Bougeault, C.; Durlet, C.; Moreira, M. A.; Virgile, R.; Vennin, E.; Agogué, H.; Hugoni, M.

    2017-12-01

    The Cretaceous Presalt travertinoid deposits of the South Atlantic are usually considered as "strange deposits" having poor equivalents in modern environments. Pastos Grandes Laguna, which is located in a 2.9 Ma caldera on the andean-bolivian Altiplano (at 4450 m), is intersected by active faults with hydrothermal fluids and presents a spherulitic plateform with similar sedimentological facies to the Presalt: halite and bedded evaporites, shrub-shaped calcites, ooids, pisolites and various stromatolites. Pastos Grandes Laguna is certainly one of the best modern analog of the Presalt for investigating the on going processes of carbonate deposition and diagenesis and the influence of biology. During two expeditions, we recovered samples of gas, water and microbial mats from the hydrothermal sources to the evaporating zones on the spherulitic plateform. These samples are being analyzed to determine 1) the influence of the gases emitted at the hydrothermal sources (chemical and isotopic composition) on the chemistry of the Laguna and the mineralogy of its sediments and 2) the role of ecosystems that develop in this environment on carbonate formation. Preliminary results on gas composition, corrected for the atmospheric contribution, indicates a magmatic source of CO2 partly mantellic associated with a small crustal contribution. Other initial results have so far indicated that CO2 gas emissions, evaporation, as well as photosynthesis and respiration play a role on water chemistry and carbonate precipitation. This study will contribute to the overall understanding of the role of organisms in sedimentation and the predictive diagenetic evolution of hydrothermal and lacustrine deposits.

  12. Determinación de enteroparásitos en lechuga Lactuca sativa en fincas dedicadas a su producción en Pasto, Nariño, Colombia

    Directory of Open Access Journals (Sweden)

    Giovanni Andrés Polo

    2016-12-01

    Full Text Available Introducción. Actualmente las verduras, como la lechuga, se recomiendan ampliamente como parte de la dieta diaria por su gran aporte nutricional, sin embargo, los consumidores al sentirse atraídos por los beneficios proporcionados por la hortaliza, están expuestos a infecciones con parásitos intestinales. Objetivo. Determinar la presencia o ausencia de enteroparásitos en la lechuga (Lactuca sativa cultivada en la zona rural de municipio de Pasto y analizar factores asociados previa caracterización de los predios. Materiales y métodos. Se realizó un estudio transversal doble ciego de tipo descriptivo. Se tomaron 105 muestras de 21 predios en el periodo de junio a diciembre de 2013 y se procesaron mediante pruebas de flotación y sedimentación. Adicionalmente, se encuestó a los propietarios para obtener información sobre las posibles variables que influyen en la aparición de enteroparásitos. Resultados. Se encontró contaminación del 100% de las muestras, las cuales presentaban estructuras parasitarias representadas en huevos y larvas correspondientes al 95,25% con quistes de Entamoeba spp., 71,43% con ooquistes de Isospora spp., 61,90% con larvas (L3 de Strongyloides stercoralis, 28,57% con huevos de Toxocara spp. y 4,76% con ooquistes de Eimeria spp. En los factores asociados mediante la prueba de Ji cuadrado, se encontró relación entre Entamoeba spp. con acequia (p= 0,008, perros (p= 0,008 y pozo séptico (p= 0,029 Isospora spp. con compostaje (p= 0,0001, perros (p= 0,0001 y babosas (p=0,002; Strongyloides stercoralis con manipulación (p= 0,003 y Toxocara spp. con el no uso de biodigestores (p= 0,002. Conclusiones. Existe contaminación de la lechuga por enteroparásitos en las áreas de cultivo del municipio de Pasto, los cuales provienen de fuentes animales y humanas como sus principales reservorios, y otros están presentes en el medio ambiente.

  13. [Spontaneous neoplasms in guinea pigs].

    Science.gov (United States)

    Khar'kovskaia, N A; Khrustalev, S A; Vasil'eva, N N

    1977-01-01

    The authors present an analysis of the data of foreign literature and the results of their personal studies of spontaneous neoplasms in 40 guinea pigs of national breeding observed during observed during a 5-year period. In 4 of them malignant tumors were diagnosed-lympholeucosis (2 cases), dermoid ovarian cysts and also cancer and adenoma of the adrenal cortex (in one animal). The neoplasms described developed in guinea pigs, aged over 4 years, and they are referred to as mostly common tumors in this species of animals.

  14. Características do pasto e acúmulo de forragem em capim-tanzânia submetido a alturas de manejo do pasto Sward characteristics and herbage accumulation of Tanzania grass submitted to sward heights

    Directory of Open Access Journals (Sweden)

    Marcos Weber do Canto

    2008-03-01

    Full Text Available O objetivo deste experimento foi avaliar alturas de manejo do pasto (20, 40, 60 e 80 cm em capim-tanzânia (Panicum maximum Jacq., em regime de lotação contínua, nas características do dossel e acúmulo de matéria seca. Os animais utilizados foram novilhos Nelore (Bos indicus, e a taxa de lotação foi variável. Foram avaliados: a massa de forragem, a massa de lâmina de folha verde, a razão folha:colmo, a composição morfológica e a taxa de acúmulo de matéria seca. O delineamento experimental foi inteiramente casualizado, com duas repetições. A massa de forragem aumentou linearmente com o aumento da altura do pasto. As médias de massa de forragem foram 2.767, 3.105, 3.657 e 4.436 kg ha-1, respectivamente, para as alturas de 20, 40, 60 e 80 cm. As taxas de acúmulo de matéria seca, a 20, 40, 60 e 80 cm, foram, respectivamente, 104, 108, 90 e 81 kg ha-1 por dia, o que indica que houve redução dessas taxas com a elevação da altura do pasto. A razão folha:colmo decresceu linearmente com o aumento da altura do pasto. Pastagens de capim-tanzânia, sob lotação contínua ao final da primavera e durante o verão, devem ser utilizadas entre 40 e 60 cm de altura.The objective of this experiment was to evaluate different sward height (20, 40, 60 e 80 cm in Tanzania grass (Panicum maximum Jacq. pastures managed under continuous stocking. The animals used were Nellore steers, and the control of sward height was done with put-and-take techniques. Evaluations were made for: forage mass, green leaf mass, leaf:stem ratio, morphological composition and dry matter accumulation rate. The experimental design was completely randomized with two replications. Forage mass increased linearly with sward height with overall mean of 2,767, 3,105, 3,657 and 4,436 kg ha-1 at sward heights 20, 40, 60 and 80 cm, respectively. Rates of dry matter accumulation decreased with increasing sward heights and were 104, 108, 90 and 81 kg ha-1 per day for sward

  15. El estatus del tío materno entre los ankave-anga: iniciaciones masculinas, ciclos de vida, género y parentesco en papúa nueva-guinea

    Directory of Open Access Journals (Sweden)

    Pascale Bonnemère

    2010-12-01

    Full Text Available Si nos atenemos a los estudios sobre las iniciaciones anga de Papúa Nueva-Guinea enfocados sólo en las actividades de los hombres, el objetivo de estos rituales era hacer crecer a los niños varones, endurecerlos en vista a posibles conflictos con las poblaciones vecinas e inculcarles los preceptos de la dominación masculina así como la legitimidad de su existencia. La etnografía de los Ankave-Anga permite una interpretación complementaria, ya que la eficacia de los rituales que marcan momentos claves de la vida masculina está garantizada por la participación de hombres y sobre todo de mujeres que pertenecen a categorías de parentescos muy específicas. El análisis de las acciones rituales que llevan a cabo estos personajes durante la iniciación de un hijo - de un hermano, de un sobrino uterino -, luego para el nacimiento de su primogénito y en fin en el momento de su muerte, revela otros aspectos fundamentales de estos rituales tales como el acceso al estatus de padre y de tío materno. Mostraremos pues que llegar a tal estatus conlleva una serie de transformaciones relacionales.According to previous analyses of Anga initiations in Papua New Guinea, which are based on male activities only, the aim of these rituals was to make boys grow, to fortify them in a context of inter-tribal conflicts as well as to drum into them the rules of male domination and to legitimate its existence. The ethnography of the Ankave-Anga permits an alternative interpretation, however, since masculine life there is punctuated with rituals whose efficacy depends on men's participation but above all on the presence of some specific kinswomen. The analysis of the ritual actions performed by these characters when a son - a brother, a uterine nephew - is initiated, and then has his first child and finally when he dies reveals other major aspects of these rituals, such as reaching parenthood and maternal uncle status, which implies that a series of relational

  16. Guinea Worm in a Frog

    Centers for Disease Control (CDC) Podcasts

    2017-03-09

    Dr. Mark Eberhard, a retired parasitologist and CDC guest researcher, discusses Guinea worm infection in a wild-caught frog.  Created: 3/9/2017 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 3/9/2017.

  17. Una nueva especie del grupo Hyloscirtus larinopygion (Amphibia: Anura: Hylidae del suroccidente de Colombia

    Directory of Open Access Journals (Sweden)

    Jonh Jairo Mueses-Cisneros

    2008-01-01

    Full Text Available Se describe una nueva especie del grupo Hyloscirtus larinopygion del Nudo de los Pastos en el departamento de Nariño, Colombia. Hyloscirtus tigrinus sp. nov. puede ser distinguida fácilmente de sus especies similares por la presencia de un tubérculo calcar carnoso y prominente, además de su distintivo patrón de coloración. Se realizan algunos comentarios sobre la coloración de la garganta y coloración ventral en Hyloscirtus caucanus, H. larinopygion, H. lindae y H. tigrinus. La coloración de la garganta es altamente variable, por lo tanto no puede ser utilizada para diagnosticar a estas especies.A new species of the Hyloscirtus larinopygion group from Nudo de los Pastos, Nariño department, Colombia is described. Hyloscirtus tigrinus sp. nov. can be distinguished easily from similar species by the presence of a fleshy and prominent calcar tubercle and by its distinctive color pattern. Some remarks on throat and ventral coloration in Hyloscirtus caucanus, H. larinopygion, H. lindae and H. tigrinus are presented. The throat coloration is highly variable and cannot be used to diagnose any of these species.

  18. La agroforestería para la producción animal; un enfoque de la Estación Experimental de Pastos y Forrajes "Indio Hatuey"

    OpenAIRE

    Martín , Giraldo; Milera , Milagros; Simón , Lionel; Hernández , David; Hernández , Ismael; Iglesias , Jesus; Gonzalez Garcia , Eliel

    2000-01-01

    [début du texte]La Estación Experimental de Pastos y Forrajes “Indio Hatuey” es la primera institución científica de la ramaagropecuaria creada por la Revolución cubana y fue fundada el 8 de marzo de 1962, con el objetivo detransferir y desarrollar tecnologías de alimentación y producción animal destinadas al Plan Ganadero CostaNorte de Cuba.

  19. Factibilidad económica de la asociación maíz-pasto en el establecimiento de un sistema silvopastoril en el piedemonte llanero de Colombia¹

    Directory of Open Access Journals (Sweden)

    V Sánchez

    Full Text Available El objetivo de la investigación fue evaluar financieramente una alternativa para el establecimiento de un sistema silvopastoril de sombra (SSP-S con leguminosas arbóreas nativas, conjuntamente con la producción de ensilaje de maíz y la renovación de praderas degradadas. Para ello se plantaron árboles en surcos y entre estos se sembró maíz, que fue cosechado para ensilar. Se seleccionaron tres especies nativas adaptadas a las condiciones de acidez del suelo (4,6, y se utilizó un arreglo factorial en bloques al azar con cuatro tratamientos, constituidos por las tres especies arbóreas a evaluar y el testigo: T O: testigo, T1: Piptadenia flava (acacia flava, T2: Cassia moschata (cañafistol y T3: Mimosa trianae (yopo, con cinco repeticiones por tratamiento, para un total de 20 unidades experimentales. Posteriormente se volvió a sembrar maíz, pero asociado con pasto, y se cosechó el maíz para ensilar, con lo que se renovó la pradera y se obtuvieron ingresos por concepto de ensilaje. Estos ingresos por la venta del ensilaje amortizaron los costos de la fase de establecimiento y generaron ganancias que hicieron viable económicamente el proyecto durante dicha fase, al lograr una utilidad neta por hectárea de USD 714, un valor presente neto (VPN de USD 407 y una tasa interna de retorno (TIR de 31,63 %. En otro tipo de SSP el costo de establecimiento puede llegar a más de USD 2 500 por hectárea y el retorno de la inversión puede tardar más de cuatro años.

  20. Cambios en el uso del suelo en el sudeste de Nicaragua, 1983-1992

    Directory of Open Access Journals (Sweden)

    Pere Pujol

    1999-12-01

    Full Text Available En este artículo se analiza los cambios del uso del suelo, en el sudeste de Nicaragua, durante el período comprendido entre 1983 a 1992. El estudio se practicó mediante la utilización de los sistemas de información geográfica y la teledetección. Se observaron tanto los cambios cuantitativos como su expresión territorial, a nivel general de todo el sudeste y a nivel particular de cada uno de sus municipios. El análisis se centró en las características de la evolución del uso del suelo en dicho período: la reducción del bosque denso y el aumento del bosque ralo y de los pastos.

  1. Estudio de productos forestales no maderables en tres mercados de Guinea Ecuatorial

    Directory of Open Access Journals (Sweden)

    Obama Ondo, Crisantos

    2001-12-01

    Full Text Available The presence of vegetable oils, wild fruits, spices, medicinal plants, plant stimulants and melongos in the markets of Ebibeyín, Bata and Malabo of Equatorial-Guinea have been studied, as remarkable non-wood forest products from these markets. The possible impact of the uses of these products on the natural environments are discussed. Some suggestions about the improvement and development of the non-wood forest products in Equatorial-Guinea are given.Se han tomado datos en los mercados de Ebibeyín, Bata y Malabo, en Guinea Ecuatorial, sobre aceites vegetales, frutos silvestres comestibles, condimentos de origen vegetal, plantas medicinales, plantas estimulantes y melongos, como principales productos forestales no maderables en venta en dichos mercados. Se discuten cuestiones generales relacionadas con el medio natural y el posible impacto en él del uso de estos productos. El estudio finaliza con unas sugerencias sobre temas relativos a la mejora y desarrollo del sector de los productos forestales no maderables en Guinea Ecuatorial.

  2. Las Micorrizas: Una alternativa de fertilización ecológica en los pastos Mycorrhizae: An alternative of ecological fertilization in pastures

    Directory of Open Access Journals (Sweden)

    Yolai Noda

    2009-06-01

    Full Text Available Debido a los efectos negativos que han causado los fertilizantes químicos en el deterioro del medio ambiente, se trabaja, desde hace algunas décadas, en la introducción de alternativas de fertilización en el manejo de los cultivos. La micorrización es una de las técnicas biológicas empleadas en muchos de ellos; sin embargo, en los pastos aún no se ha logrado extenderla ampliamente en la producción y los estudios han estado dirigidos a algunas leguminosas y muy pocas gramíneas. Las micorrizas permiten una aplicación exitosa mediante el recubrimiento de las semillas. Por otra parte, las relaciones micorrízicas pueden ser la clave para disminuir la cantidad de fertilizantes (especialmente fosfatos que debe aplicarse para obtener buenos rendimientos; en los suelos con altos contenidos de P la inoculación con micorriza incrementa el crecimiento y el establecimiento temprano de los cultivos. Las plantas desarrollan una calidad biológica superior, en cuanto a mayor altura, vigor y área foliar, y se incrementan los rendimientos (entre 15 y 50%. Protege las raíces contra ciertos hongos patógenos. Además, el biofertilizante permite ahorrar hasta un 50% del volumen de los productos químicos necesarios, lo que favorece la reducción de los insumos y de los costos, e influye en el ejercicio de una agricultura sostenible y ecológicamente más sana.Because of the negative effects caused by chemical fertilizers on the deterioration of the environment, work has been done, for some decades, in the introduction of fertilization alternatives in crop management. Mycorrhization is one of the biological techniques used in many of them; however, in pastures it has not been widely extended in production and the studies have aimed at legumes and very few grasses. Mycorrhizae allow successful application by means of seed covering. On the other hand, mycorrhizal relationships can be the key to decrease the amount of fertilizers (especially phosphates

  3. Dermatophytes in pet Guinea pigs and rabbits.

    Science.gov (United States)

    Kraemer, A; Mueller, R S; Werckenthin, C; Straubinger, R K; Hein, J

    2012-05-25

    The frequency of dermatophytes in pet Guinea pigs and rabbits. To determine the frequency and types of dermatophytes in pet Guinea pigs and rabbits. First, 2153 samples collected from pet Guinea pigs (n=1132) and rabbits (n=1021) with suspected dermatophytosis and submitted to three different laboratories for fungal culture were analysed. Subsequently, healthy Guinea pigs and rabbits, animals with skin lesions and with noncutaneous diseases were examined prospectively for dermatophytes. Trichophyton (T.) mentagrophytes was the most common fungal species isolated (91.6% and 72.3% of positive cultures from Guinea pigs (n=431) and rabbits (n=83), respectively). Animals with positive fungal culture did not show any gender predisposition, but affected animals were younger than those with negative fungal culture (PGuinea pigs and 0/140 healthy rabbits. In addition, fungal cultures of Guinea pigs with skin lesions (n=26) and other diseases (n=25) were positive in 7.7% and 8.0% respectively. Samples collected from 17 rabbits with skin lesions and 32 rabbits with noncutaneous disease were all negative in culture. T. mentagrophytes is the most common dermatophyte in pet Guinea pigs and rabbits, asymptomatic carriers are regularly seen in Guinea pigs, but not in rabbits. Copyright © 2011 Elsevier B.V. All rights reserved.

  4. The Vaginal Microbiota of Guinea Pigs

    OpenAIRE

    Hafner, L. M.; Rush, C. M.; Timms, P.

    2011-01-01

    The vaginae of four guinea pigs were swabbed and samples cultured aerobically on horse blood agar, in 5 per cent carbon dioxide on MRS agar or anaerobically on anaerobic horse blood agar. Vaginal microbiota consisted almost exclusively of gram-positive bacteria including Corynebacterium, Streptococcus, Enterococcus, Staphylococcus and Lactobacillus species.Keywords: guinea pigs, vaginal microbiota, vaginal vaccines.

  5. African Journals Online: Papua New Guinea

    African Journals Online (AJOL)

    African Journals Online: Papua New Guinea. Home > African Journals Online: Papua New Guinea. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Browse By Category · Browse Alphabetically · Browse By Country · List All Titles · Free to read Titles This Journal is ...

  6. Guinea Pigs: Versatile Animals for the Classroom

    Science.gov (United States)

    Barman, Charles R.

    1977-01-01

    Guinea pigs are presented as versatile classroom animals. Suggestions for animal behavior and genetics studies are given. Also included is information concerning sex determination and the breeding of guinea pigs, and hints on keeping these animals in the classroom. References and illustrations complete the article. (MA)

  7. Endocrine tumours in the guinea pig.

    Science.gov (United States)

    Künzel, Frank; Mayer, Jörg

    2015-12-01

    Functional endocrine tumours have long been thought to be rare in guinea pigs, although conditions such as hyperthyroidism and hyperadrenocorticism have been documented with increasing frequency so the prevalence of hormonal disorders may have been underestimated. Both the clinical signs and diagnosis of hyperthyroidism in guinea pigs appear to be very similar to those described in feline hyperthyroidism, and methimazole has been proven to be a practical therapy option. Hyperadrenocorticism has been confirmed in several guinea pigs with an adrenocorticotropic hormone stimulation test using saliva as a non-invasive sample matrix; trilostane has been successfully used to treat a guinea pig with hyperadrenocorticism. Insulinomas have only rarely been documented in guinea pigs and one animal was effectively treated with diazoxide. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Adenocarcinoma of the gallbladder in guinea pigs

    International Nuclear Information System (INIS)

    Hoch-Ligeti, C.; Congdon, C.C.; Deringer, M.K.; Stewart, H.L.

    1979-01-01

    Adenocarcinoma of the gallbladder developed in 17 of 68 untreated and in 26 of 83 irradiated guinea pigs of inbred strains 2 and 13. The carcinomas spread widely by direct extension and through lymphatic and blood vessels to lymph nodes, mesenteries, omenta, abdominal wall, liver, lungs, bones, and spleen. Whole-body exposure to gamma or x radiation increased both the number of tumors and metastases in male inbred guinea pigs but not in females. Significantly fewer (9 of 98) noninbred than inbred guinea pigs developed gallbladder carcinomas after irradiation. In 9 untreated noninbred guinea pigs gallbladder carcinomas were not found. Inasmuch as the effect of irradiation was not dose-dependent, an indirect systemic effect of irradiation was postulated. This is the first report on the occurrence of spontaneous gallbladder adenocarcinomas in guinea pigs

  9. Efecto de sistemas de labranza en luvisoles dedicados a la producción de pastos

    Directory of Open Access Journals (Sweden)

    Santa L Leyva

    Full Text Available Se realizó un experimento con el objetivo de determinar la influencia de los sistemas de labranza en las propiedades de los suelos luvisoles, en el municipio de Las Tunas. Los indicadores evaluados fueron: la densidad aparente (r b, la porosidad total (Pt, la porosidad de aireación (Pa, la materia orgánica (MO y la densidad y biomasa de oligoquetos. Se muestrearon unidades experimentales, en un diseño de parcelas divididas y tres repeticiones, a las profundidades de 0-5, 5-20 y 20-30 cm. Los contenidos de MO y de densidad y biomasa de oligoquetos se evaluaron hasta los 20 cm. Se consideraron dos sistemas: labranza tradicional (LT y labranza sin inversión del prisma (LSP en comparación con un control sin labranza (NL. La LSP produjo la menor densidad y la mayor cantidad de espacios porosos en la matriz del suelo, con valores que difirieron significativamente de la LT y la NL, y favoreció el desarrollo de los oligoquetos hasta la profundidad de 20 cm. El mantenimiento de las capas densas en la profundidad de 20-30 cm en la LT disminuyó la porosidad total y restringió el desarrollo de las raíces, con valores de porosidad de aireación muy por debajo del 10 %. Se concluye que la labranza tradicional afectó el contenido de MO a corto plazo, y mantuvo las capas compactas en el horizonte subyacente, por lo que la continuidad de esta práctica en las áreas ganaderas de la región no se justifica ni a corto ni a largo plazo.

  10. 9 CFR 113.38 - Guinea pig safety test.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Guinea pig safety test. 113.38 Section... Standard Procedures § 113.38 Guinea pig safety test. The guinea pig safety test provided in this section... be injected either intramuscularly or subcutaneously into each of two guinea pigs and the animals...

  11. Impact of land management on soil macrofauna in the upper reaches of the Guames Lake, Pasto, Colombia

    International Nuclear Information System (INIS)

    Coral E, Dilia M; Bonilla C, Carmen R

    1998-01-01

    The following investigation was carried out in order to evaluate the biomass, abundance, vertical distribution and diversity of the species it compared the results in three sites: a secondary forest, a field kikuyu and an onion crop field all at the Refugio Cristalino Natural Reserve, Pasto, Narino. The investigation followed the recommendations of the tropical soil biology and fertility program at the some way samples were taken to evaluate the physical and chemical characteristics of the sites. The results showed that the greatest abundance of organisms were found in the litter and 0-10 cm layer of soil in the secondary forest; of this abundance the Myriapodans formed the greatest part in number and in biomass. These was greater species diversity here than in the others sites, possibly due to the prime feeding and habitat conditions, notable in the organic matter in different stages of decomposition. In the Kikuyu and onion fields earthworms found in the first 10 cm profile principally represented the abundance and biomass. in general the use of the land affects the presence or absence of organisms that are fundamental in the process of soil evolution

  12. Suplementação com cromo e desempenho reprodutivo de vacas zebu primíparas mantidas a pasto

    Directory of Open Access Journals (Sweden)

    Aragón V.E.F.

    2001-01-01

    Full Text Available Foram utilizadas 126 vacas zebu primíparas, mantidas a pasto durante a estação de monta, divididas em dois grupos, suplementado (72 animais e controle (54 animais, com o objetivo de estudar a influência da suplementação com cromo (Cr sobre algumas características reprodutivas. Utilizou-se como fonte de cromo a levedura Sacharomices cerevisae adicionada à mistura mineral (0,017% Cr. Não houve diferença significativa nos pesos das crias ao nascer e ao final do experimento. O peso final das vacas foi maior no grupo suplementado (428,5kg vs. 380,5kg. A porcentagem de animais em estro foi maior no grupo suplementado (98,6% vs. 88,7% e a porcentagem total de prenhez apresentou apenas tendência em ser maior no grupo suplementado (87,5% vs. 75,47%. O intervalo parto-primeiro cio foi menor nos animais que receberam Cr (120,5 dias vs. 142,8 dias. O número de doses de sêmen por vaca inseminada (1,57 e por vaca gestante (1,85 no grupo controle foi semelhante ao de vacas inseminadas (1,69 e gestantes (1,90 no grupo suplementado.

  13. Experimental aerosolized guinea pig-adapted Zaire ebolavirus (variant: Mayinga) causes lethal pneumonia in guinea pigs.

    Science.gov (United States)

    Twenhafel, N A; Shaia, C I; Bunton, T E; Shamblin, J D; Wollen, S E; Pitt, L M; Sizemore, D R; Ogg, M M; Johnston, S C

    2015-01-01

    Eight guinea pigs were aerosolized with guinea pig-adapted Zaire ebolavirus (variant: Mayinga) and developed lethal interstitial pneumonia that was distinct from lesions described in guinea pigs challenged subcutaneously, nonhuman primates challenged by the aerosol route, and natural infection in humans. Guinea pigs succumbed with significant pathologic changes primarily restricted to the lungs. Intracytoplasmic inclusion bodies were observed in many alveolar macrophages. Perivasculitis was noted within the lungs. These changes are unlike those of documented subcutaneously challenged guinea pigs and aerosolized filoviral infections in nonhuman primates and human cases. Similar to findings in subcutaneously challenged guinea pigs, there were only mild lesions in the liver and spleen. To our knowledge, this is the first report of aerosol challenge of guinea pigs with guinea pig-adapted Zaire ebolavirus (variant: Mayinga). Before choosing this model for use in aerosolized ebolavirus studies, scientists and pathologists should be aware that aerosolized guinea pig-adapted Zaire ebolavirus (variant: Mayinga) causes lethal pneumonia in guinea pigs. © The Author(s) 2014.

  14. Tóxidad de cadmio y plomo en pasto tanner Brachiaria arrecta

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    Manuel Peláez

    2014-12-01

    Full Text Available Se evaluó fisiopatías en Brachiaria arrecta expuestas a contenidos tóxicos de cadmio y plomo en el invernadero de la Universidad Nacional de Colombia Sede Palmira, se doparon plántulas sanas de 20 días de edad bajo cultivo hidropónico. El objetivo fue describir síntomas crecientes por intoxicación con el diseño de una escala valorativa de daños crecientes, adicionalmente se evaluaron cambios histológicos de raíz tallo y hojas mediante microscopia de luz de alta resolución (MLAR. Las plantas fueron sometidas a estrés abiótico con los tratamientos de sales de CdCl2 en concentración de 10 ppm, y de Pb (NO32 500 ppm, respectivamente. Los resultados principales mostraron un mayor detrimento cuando las plantas fueron expuestas al metal plomo que al cadmio en las raíces, tallos y hojas. Se apreciaron deformaciones extremas en paredes celulares de las células del cortex y en la endodermis de la raíz; en las hojas los daños fueron notorios para ambos metales sobre todo en el parénquima clorofílico y en la deformación de paredes de la epidermis tanto en haz como envés; en los tallos aparecieron deformaciones de las células del parénquima de relleno cuando ocurre bioacumulación intensa de plomo. Estos resultados sugieren que la bioconcentración en los tejidos vegetales ocasiona trastornos histológicos, que pueden involucrar una red trófica y por consiguiente consecuencias en la Salud Pública.

  15. Efecto de un bioinoculante a partir de consorcios microbianos nativos fosfato solubilizadores, en el desarrollo de pastos Angleton (Dichantium aristatum Titulo en ingles: Effect of bio-inoculant from microbial consortia phosphate solu

    Directory of Open Access Journals (Sweden)

    Cecilia Lara Mantilla

    2015-01-01

    Full Text Available El objetivo fue evaluar la capacidad solubilizadora de fosfatos de consorcios formados por bacterias nativas de los géneros Burkholderia cepacia, Pseudomonas sp, Pseudomonas luteola y Pantoea sp, con el fin de encontrar el más eficiente. Se realizaron pruebas de antagonismo entre las cepas y se formaron consorcios probando todas las combinaciones posibles en las concentraciones de 106, 107, 108 UFC/mL. Se realizaron evaluaciones cualitativas y cuantitativas de la solubilización de fosfatos y teniendo en cuenta éstos resultados, se preparó un bioinoculante el cual fue evaluado en semillas de plantas de pasto angleton (Dichantium aristatum a escala de laboratorio, utilizando un diseño estadístico completamente al azar (DCA con 3 tratamientos y 5 repeticiones: Tratamiento 1 semillas (control, Tratamiento 2, semillas tratadas con el consorcio de microorganismos seleccionado y Tratamiento 3, semillas tratadas con fertilizantes comerciales DAP y Urea. Se evaluaron las variables número de hojas, área foliar, longitud de la planta, longitud de la raíz y peso seco de todas las plantas. Los resultados de la prueba de antagonismo indicaron que no existe inhibición en el crecimiento de las cepas evaluadas, por lo tanto se formaron consorcios los cuales mostraron mayor eficiencia en la solubilización del fósforo, destacándose el consorcio formado por Pantoea sp + Pseudomonas sp a una concentración de 108 UFC/mL y con índices de solubilización de 5,3 y 842 ppm. En las plantas se evidenció un incremento significativo en los parámetros peso seco y área foliar usando el consorcio microbiano, indicando mayor beneficio en comparación con el control.

  16. Application of decision trees in the identification of patterns of fatal injuries by external cause in the municipality of Pasto, Colombia

    Directory of Open Access Journals (Sweden)

    Ricardo Timaran-Pereira

    2017-12-01

    Full Text Available Introduction: The Pan American Health Organization (PHO and the World Health Organization (WHO accepted, since the year 1993 and 1996 respectively, that violence is a public health problem, a situation that is corroborated in the report on violence and health, in which Latin America presented a homicide rate of 18 per 100,000 people, and it is considered one of the most violent regions in the world. Objective: To detect criminal patterns with data mining techniques in the Crime Observatory of the municipality of Pasto (Colombia. Materials and methods: Cross Industry Standard Process for Data Mining (CRISP-DM was applied, which is one of the methodologies used in the development of data mining projects in academic and industrial environments. The source of information was the Crime Observatory of the municipality of Pasto, where the historical clean and transformed figures on the injuries of external cause (fatal and nonfatal recorded in 11 years are stored. Results: A decision tree-based classification model was built that allowed the discovery of patterns of deaths from external causes. In the case of homicide, these happened mostly in the commune 5 in Pasto under the following circumstances: during the weekends, in the early morning, in the second semester of the year and in the public thoroughfare; besides, the victims were adult men of various professions; and the cause of the homicides were quarrels and they were produced with a fire gun. Conclusion: The generated knowledge will help government and security agencies make effective decisions regarding the implementation of crime prevention and citizen security plans

  17. CUANTIFICACIÓN DE MERCURIO EN PASTO TRATADO CON BIOSÓLIDOS POR ESPECTROFOTOMETRÍA DE ABSORCIÓN ATÓMICA CON GENERADOR DE HIDRUROS

    Directory of Open Access Journals (Sweden)

    Amilvia Yanett Ortiz Villa

    Full Text Available Los subproductos sólidos de carácter orgánico generados por los procesos de tratamiento de aguas residuales municipales son utilizados comúnmente como fuente de nutrientes para tratamiento de suelos. Estos biosólidos contienen nutrientes contaminantes inorgánicos como el mercurio, el cual es empleado para el estudio, por el conocimiento ampliamente documentado de su toxicidad en sistemas vivos y sus efectos en seres humanos. En este mismo sentido, y puesto que las plantas constituyen la base de la cadena trófica, se centra la atención sobre el pasto, debido a su fácil crecimiento y uso como pastura de ganadería. El mercurio en pasto fue cuantificado mediante espectrofotometría de absorción atómica utilizando la técnica de generador de hidruros con vapor frío. Se verificaron algunos parámetros de validación con criterios de aceptación deseables tales como linealidad (r² = 0,999, exactitud (R = 93,14-96,12%, precisión (CV = 1,11-1,69%, límites de detección y cuantificación (0,001-0,003 ppb. Se evidenció que las concentraciones de mercurio se encuentran por debajo de los valores máximos permitidos por la normatividad Internacional, presentando el biosólido como fuente confiable de nutrientes para el agro. De esta forma se establece una metodología que puede ser una buena herramienta para la determinación analítica de mercurio en pastos.

  18. Efeito residual da jitirana, flor-de-seda e mata-pasto no cultivo da rúcula em sucessão a beterraba

    Directory of Open Access Journals (Sweden)

    Alany Moisa Bezerra Almeida

    2015-05-01

    Full Text Available O efeito residual da adubação anterior em cultivos subsequente é de suma importância, uma vez que a fertilização do solo em uma atividade tão intensa, como a olericultura, aumentaria o custo de produção sempre que fosse implantada uma nova cultura. Este trabalho foi conduzido na Fazenda Experimental Rafael Fernandes, localizada no distrito de Alagoinha, zona rural de Mossoró-RN, no período de novembro a dezembro de 2011. Objetivou-se avaliar o efeito residual da jitirana, flor-de-seda e mata-pasto no cultivo da rúcula em sucessão a beterraba. A beterraba foi plantada em parcelas de 1,2 x 1,2 m e adubado com espécies espontâneas, nas quantidades: 0,6; 1,2; 1,8; 2,4 e 3,0 kg m-2 de canteiro. Após a retirada da beterraba, semearam-se sementes de rúcula nas parcelas referente aos tratamentos acima citados. O delineamento experimental foi em blocos casualizados com três repetições, no esquema fatorial 5 x 3, sendo cinco quantidades e três tipos de adubos verdes (jitirana; flor-de-seda e mata-pasto. A cultivar de rúcula plantada foi a Cultivada. As características avaliadas foram: altura e número de folhas por planta, massa fresca, número de molho de rúcula e massa seca. Houve interação dos fatores estudados para massa fresca de rúcula e número de molhos. O efeito residual na quantidade de 3,0 kg m-2 de canteiro contribuiu para a massa fresca de rúcula da ordem de 762; 684 e 713 g m-2 de canteiro, equivalente a 6,0; 5,0 e 5,0 molhos de rúcula para jitirana, flor-de-seda e mata-pasto, respectivamente. Jitirana, flor-de-seda e mata-pasto são espécies promissoras para ser utilizadas como adubo verde na produção de hortaliças.

  19. The oil boom in Equatorial Guinea

    International Nuclear Information System (INIS)

    Frynas, J.G.

    2004-01-01

    In less than a decade, Equatorial Guinea has transformed itself from an African backwater into one of the world's fastest growing economies and a sought-after political partner in the Gulf of Guinea. The sole reason for this transformation has been the discovery of oil and gas. This article outlines the rise of Equatorial Guinea as one of Africa's leading oil-producing countries and investigates the political, economic and social effects of becoming a petro-state. The article is based on the author's field research in Equatorial Guinea in the autumn of 2003 and interviews with senior oil company staff, government officials and staff of international organizations as well as secondary sources. This research demonstrates how reliance on oil and gas exports can lead to profound changes in a country's political economy. (author)

  20. Polysaccharide prodigiosan therapy of irradiated guinea pigs

    International Nuclear Information System (INIS)

    Chertkov, K.S.; Mosina, Z.M.; Khramchenkova, S.P.

    1976-01-01

    In the experiments with irradiated guinea-pigs, a therapeutic action of prodigiosan has been detected. A parenteral administration of the preparation (125 to 500 μg/kg) within the interval from 15 min to 6 hours after a short-term exposure increased the survival of animals to 50%. Prodigiosan administered to guinea-pigs given prolonged irradiation failed to affect the survival rate

  1. English and Tok Pisin (New Guinea Pidgin English) in Papua New Guinea.

    Science.gov (United States)

    Romaine, Suzanne

    1989-01-01

    Tok Pisin, New Guinea Pidgin English, is becoming increasingly important as a "lingua franca" in Papua New Guinea, even though English is the country's official language. Urban versus rural and spoken versus written varieties of the pidgin are examined, and the influence of English on Tok Pisin is investigated. 73 references. (Author/CB)

  2. Efficiency in the reduction of chromium by a wild bacterium in a Batch treatment type using residual water substrate from the municipality of Pasto, Colombia

    Directory of Open Access Journals (Sweden)

    Deisy Lorena Guerrero Ceballos

    2017-05-01

    Full Text Available Objective: To evaluate the efficiency in the reduction of chromium in a Batch treatment type, using municipal residual water substrate inoculated with a wild bacterium. Materials and methods: The reduction percentage of hexavalent chromium of three wild bacteria previously isolated from residual water from the Pasto River was verified at laboratory scale (Bacillus thuringiensis, Bacillus amyloliquefaciens and Paenibacillus sp.; the isolated that showed the highest percentage of reduction of Cr was selected and was subjected to different treatments. The analysis of results was done using descriptive statistics. Results: B. thuringiensis, B. amyloliquefaciens, and Paenibacillus sp., presented percentages of reduction of Cr (VI of 82,01%; 80,85% and 79,27%, respectively. It was determined that the third treatment (nonsterile water from the Pasto River with B. thuringiensis presented significant differences with regard to the other (p = 0.0001 α = 0.05, concluding that B. thuringiensis reduces in greater proportion the Cr (VI. The results found in this research are promising in the field of bioremediation of contaminated effluents with Chrome since they may be taken as the basis for implementing strategies of bioremediation on a large scale. Conclusion: The bacteria B. thuringiensis presented high efficiency in the reduction of hexavalent chromium (99.42% when implemented in a treatment at laboratory scale of residual nonsterile water.

  3. Estrutura do pasto de capim-braquiária com variação de alturas Structure of signal grass pasture with height variation

    Directory of Open Access Journals (Sweden)

    Manoel Eduardo Rozalino Santos

    2010-10-01

    Full Text Available Este trabalho foi conduzido com o objetivo de avaliar as características estruturais da Brachiaria decumbens cv. Basilisk. Foram avaliadas quatro alturas de plantas (10, 20, 30 e 40 cm dentro de uma mesma pastagem, em um delineamento experimental de blocos casualizados com duas repetições. A variação na altura inicial das plantas no pasto de capim-braquiária apresentou resposta linear e negativa. As massas de lâmina foliar verde, colmo verde, material morto, forragem verde e forragem total aumentaram linearmente com a altura das plantas no pasto. Contrariamente, houve redução linear na relação lâminas foliares verdes/colmos verdes com a altura das plantas no pasto (de 1,16 para 0,58. A altura das plantas no pasto teve efeito mais pronunciado sobre o aumento das massas de colmo verde e material morto que sobre a massa de lâmina foliar verde. A densidade volumétrica de lâminas foliares verdes decresceu (de 97,09 para 39,07 kg/cm.ha de MS com a altura das plantas no pasto. Mesma resposta ocorreu para as densidades volumétricas de forragem verde (de 181,62 para 107,85 kg/cm.ha de MS e forragem total (de 283,39 para 195,72 kg/cm.ha de MS. Por outro lado, não houve efeito da altura das plantas no pasto sobre as densidades volumétricas de colmos verdes e material morto, que apresentaram valores médios de 80,95 e 95,61 kg/cm.ha de MS, respectivamente. A altura das plantas no pasto teve efeito quadrático sobre a interceptação de luz pelo dossel. Áreas da pastagem com alturas de 10, 20, 30 e 40 cm apresentaram valores de interceptação luminosa de 56,15; 85,16; 93,39 e 96,57%, respectivamente. Ocorre variação espacial e temporal na estrutura do pasto de B. decumbens manejado sob lotação contínua.This study was carried out aiming to evaluate the structural characteristics of Brachiaria decumbens cv. Basilisk pasture. Four heights of plants (10, 20, 30 and 40 cm were evaluated within the same grazing area in a randomized block

  4. Influencia del polvo del horno de cemento sobre plantas y animales

    Directory of Open Access Journals (Sweden)

    Pajenkamp, H.

    1963-06-01

    Full Text Available Not availableEl pretendido «polvo de cemento» de los alrededores de las fábricas no es de cemento, sino que se compone, en su mayor parte, de caliza molida muy fina. Es, por tanto, un producto intermedio de la fabricación del cemento. Sería mucho más correcto llamarle «polvo del horno de cemento». Continuamente alegan los agricultores que este «polvo del horno de cemento» es dañino para plantas y animales, y en los últimos años, para probar la validez de tales asertos, se han llevado a cabo numerosas investigaciones científicas para estudiar los efectos de este polvo. Estamos hoy día en posesión de una serie· de trabajos muy completos que dan una idea clara sobre el efecto del polvo del horno de cemento. Los animales domésticos útiles (por ejemplo: corderos, vacas de leche no sufren ningún perjuicio clínico, ninguna deficiencia de su estado general, a pesar de serie administradas grandes dosis de polvo. Los ensayos agrícolas con plantas útiles (avena, trébol, remolacha azucarera, pastos que han sido efectuados durante varios años, han demostrado que ni siquiera cantidades elevadas de polvo provocan perjuicios ni influencias nefastas sobre el crecimiento y sobre la recolección.

  5. Simulación del balance anual en sistemas de pastoreo bovino

    Directory of Open Access Journals (Sweden)

    Verena Torres Cárdenas

    2002-07-01

    Full Text Available Este estudio parte de una abstracción de los sistemas de pastoreo vacuno en Cuba en cuanto a la dinámica del N, P y K, determinando los procesos relevantes para el modelo de simulación. Para  tales procesos se define cómo estimar la cuantía de su contribución como fuente de entrada o salida de los nutrientes por tres posibles métodos: modelos de ajustes, procedimientos descriptivos (fórmulas de cálculos o con valores informados en la literatura. Las componentes del modelo de simulación fueron: estimación del N, P y K aportado por  bostas,  orina,  hojarasca de los árboles,  hojarasca de los pastos, removilización y contenido en la suplementación utilizada.  Estimación del N aportado por la lluvia  y  volatilizado por bostas y orina.  Estimación del N, P y K en la leche producida,  en la ganancia de peso vivo, en los animales que salen del sistema y el  extraído por los pastos. El soporte informático del modelo se programó en Borland Delphi versión 4.0 utilizando el método de Prototipo Orientado a Objeto y el mismo garantiza la rapidez, exactitud y precisión en las estimaciones que se hacen, lo que se comprobó mediante una adecuada estrategia de validación efectuada hasta la introducción del software en la investigación, la docencia y la producción con resultados muy positivos.

  6. A new species of Rhododendron from New Guinea (Ericaceae)

    NARCIS (Netherlands)

    Kores, Paul

    1978-01-01

    The Star Mountains of New Guinea are situated at the geographic center of the Island of New Guinea extending on both sides of the Indonesian-Papua New Guinea border. Access to these mountains from either side of the border which divides the island is relatively difficult and as a result few

  7. Development of a Guinea Pig Lung Deposition Model

    Science.gov (United States)

    2016-01-01

    Development of a Guinea Pig Lung Deposition Model Distribution Statement A. Approved for public release; distribution is unlimited. January...4 Figure 2. Particle deposition in the lung of the guinea pig via endotracheal breathing...Particle deposition in the lungs of guinea pigs via nasal breathing. ......................................... 12 v PREFACE The research work

  8. Malignant transformation of guinea pig cells after exposure to ultraviolet-irradiated guinea pig cytomegalovirus

    International Nuclear Information System (INIS)

    Isom, H.C.; Mummaw, J.; Kreider, J.W.

    1983-01-01

    Guinea pig cells were malignantly transformed in vitro by ultraviolet (uv)-irradiated guinea pig cytomegalovirus (GPCMV). When guinea pig hepatocyte monolayers were infected with uv-irradiated GPCMV, three continuous epithelioid cell lines which grew in soft agarose were established. Two independently derived GPCMV-transformed liver cells and a cell line derived from a soft agarose clone of one of these lines induced invasive tumors when inoculated subcutaneously or intraperitoneally into nude mice. The tumors were sarcomas possibly derived from hepatic stroma or sinusoid. Transformed cell lines were also established after infection of guinea pig hepatocyte monolayers with human cytomegalovirus (HCMV) or simian virus 40 (SV40). These cell lines also formed colonies in soft agarose and induced sarcomas in nude mice. It is concluded that (i) GPCMV can malignantly transform guinea pig cells; (ii) cloning of GPCMV-transformed cells in soft agarose produced cells that induced tumors with a shorter latency period but with no alteration in growth rate or final tumor size; and (iii) the tumors produced by GPCMV-and HCMV-transformed guinea pig cells were more similar to each other in growth rate than to those induced by SV40-transformed guinea pig cells

  9. Degradabilidad ruminal in vitro de ensilajes de pasto saboya (Panicum maximum jacq. con diferentes niveles de inclusión de cáscara de maracuyá (Passiflora edulis sims.

    Directory of Open Access Journals (Sweden)

    Alexandra Barrera-Álvarez

    2017-12-01

    Full Text Available La investigación tuvo como objetivo evaluar la composición y degradabilidad ruminal in vitro de la materia seca, materia orgánica y materia inorgánica de los ensilajes de pasto saboya con los tratamientos T1: Pasto saboya 90%+cáscara de maracuyá 10%; T2: Pasto saboya 80%+cáscara de maracuyá 20%; T3: Pasto saboya 70%+cáscara de maracuyá 30% y T4: Pasto saboya 60%+cáscara de maracuyá 40%. Se utilizó el método de microensilaje (silos PVC de 3 kg de capacidad provistos de una válvula bunsen y mecanismo de extracción de efluentes y la técnica de degradación in vitro con el sistema de incubación DAISY II, empleando el líquido ruminal de bovinos brahman de 500±25 kg de peso promedio. Se aplicó un Diseño Completamente al Azar con seis repeticiones. El nivel de inclusión de maracuyá mejoró el contenido de nutrientes en el ensilaje, incrementando la materia orgánica, la proteína bruta y la grasa bruta y disminuyendo las fracciones de fibra. La degradabilidad de la materia seca, materia orgánica y materia inorgánica fue superior (p

  10. Lack of effect of ivermectin on prepatent guinea-worm

    DEFF Research Database (Denmark)

    Issaka-Tinorgah, A.; Magnussen, P.; Bloch, P.

    1994-01-01

    The effect of ivermectin on prepatent guinea-worm was tested in a single-blind placebo-controlled trial; 400 adults were randomly allocated to a single dose of ivermectin (150 µg/kg) or placebo. Fifty-four of the 385 participants who were followed for 15 months developed a total of 69 emergent...... guinea-worms. There was no significant differencein the proportion of persons with emergent guinea-worms between the 2 treatment groups; 58% appeared in males. 80% of emergent guinea-worms were located below the knee. Migration of guinea-worms in the tissues was not affected. It is concluded...

  11. Vitamin C deficiency in weanling guinea pigs

    DEFF Research Database (Denmark)

    Lykkesfeldt, Jens; Trueba, Gilberto Perez; Poulsen, Henrik E.

    2007-01-01

    Neonates are particularly susceptible to malnutrition due to their limited reserves of micronutrients and their rapid growth. In the present study, we examined the effect of vitamin C deficiency on markers of oxidative stress in plasma, liver and brain of weanling guinea pigs. Vitamin C deficiency...... increased, while protein oxidation decreased (P¼0003). The results show that the selective preservation of brain ascorbate and induction of DNA repair in vitamin C-deficient weanling guinea pigs is not sufficient to prevent oxidative damage. Vitamin C deficiency may therefore be particularly adverse during...

  12. EVALUACIÓN DE LA BIOACUMULACIÓN Y TOXICIDAD DE CADMIO Y MERCURIO EN PASTO LLANERO (Brachiaria dictyoneura)

    OpenAIRE

    ARROYAVE Q, Catalina; ARAQUE M, Pedronel; PELAEZ J, Carlos A

    2010-01-01

    Se estudia la transferencia de mercurio y cadmio desde el sistema suelo hacia Brachiaria dictyoneura en función de la capacidad bioindicadora y bioacumuladora. Durante la evaluación de cadmio en B. dictyoneura se observó una disminución en el crecimiento del 65%. La toxicidad de cadmio se correlaciona con la acumulación consecutiva del catión durante el tiempo de monitoreo. En el tratamiento con mercurio, a partir de los 30 días de exposición, se observó disminución en el contenido del catión...

  13. Prolactin family of the guinea pig, Cavia porcellus.

    Science.gov (United States)

    Alam, S M Khorshed; Konno, Toshihiro; Rumi, M A Karim; Dong, Yafeng; Weiner, Carl P; Soares, Michael J

    2010-08-01

    Prolactin (PRL) is a multifunctional hormone with prominent roles in regulating growth and reproduction. The guinea pig (Cavia porcellus) has been extensively used in endocrine and reproduction research. Thus far, the PRL cDNA and protein have not been isolated from the guinea pig. In the present study, we used information derived from the public guinea pig genome database as a tool for identifying guinea pig PRL and PRL-related proteins. Guinea pig PRL exhibits prominent nucleotide and amino acid sequence differences when compared with PRLs of other eutherian mammals. In contrast, guinea pig GH is highly conserved. Expression of PRL and GH in the guinea pig is prominent in the anterior pituitary, similar to known expression patterns of PRL and GH for other species. Two additional guinea pig cDNAs were identified and termed PRL-related proteins (PRLRP1, PRLRP2). They exhibited a more distant relationship to PRL and their expression was restricted to the placenta. Recombinant guinea pig PRL protein was generated and shown to be biologically active in the PRL-responsive Nb2 lymphoma cell bioassay. In contrast, recombinant guinea pig PRLRP1 protein did not exhibit PRL-like bioactivity. In summary, we have developed a new set of research tools for investigating the biology of the PRL family in an important animal model, the guinea pig.

  14. Parasitismo intestinal en una escuela primaria de Bata, Guinea Ecuatorial Intestinal parasitism in an elementary school in Bata, Equatorial Guinea

    Directory of Open Access Journals (Sweden)

    William Quintero Pérez

    2008-06-01

    Full Text Available Con el objetivo de precisar el grado de parasitismo intestinal según la edad, así como identificar los parásitos intestinales más frecuentes en la escuela primaria "Amiguitos de Jesús" de la ciudad de Bata, Guinea Ecuatorial, en el periodo septiembre 2002 a Junio 2003, se realizó un estudio descriptivo y prospectivo. En el mismo se encontró que el grado de parasitismo intestinal fue de un 29.9 % del total de análisis realizados, predominando sobre todo en los menores de 6 años; un 44.7% de éstos estaban infestados. Se encontró un predominio de los vermes: el más frecuente fue Trichuris trichiura, para el 95.1 % del total de niños infestados, y el áscaris lumbricoides, con un 75.6% del total de 41 infestados. De los protozoos, el más frecuente fue la ameba, existiendo en un 17.07 % de los infestados. El grado de infestación encontrado es elevado, requiriéndose programas educativos para fomentar el cuidado de los hábitos higiénicos de los niños. El predominio de los parásitos encontrados podría deberse a la contaminación de la tierra con heces fecales y a la falta de un adecuado tratamiento de las aguas para el consumo, y de infraestructura sanitaria.With the aim of defining exactly the grade of intestinal parasitism according to age, as well as identifying the most frequent intestinal parasites in "Amiguitos de Jesús" Elementary School in Bata City, Equatorial Guinea, during septembre 2002 to June 2003, a descriptive, prospective study is carried out. In the study it was found that the parasitism grade was 29.9% from the total of the analysis performed, predominating in those under 6 years of age: 44.7% of these cases were infected. A prevalence of vermes was observed, Trichuris trichiuria was the most frequent species found, representing 95,1% from the total of infected children and Ascaris lumbricoides 75,6% from the total of 41 infected. Among protozoa, amoeba was the most frequent specie observed, being present in 17

  15. La productividad de cabras ramoneando Leucaena leucocephala en las áreas semi áridas del noroeste de Venezuela

    OpenAIRE

    Clavero, Tyrone; Razz, Rosa

    2009-01-01

    Un experimento de campo fue realizado en las áreas semiáridas del noroeste de Venezuela para evaluar tres tipos de dietas en cabras (pastoreo de gramíneas (pasto buffel); pastoreo de gramíneas + 0.3 kg/día de alimento comercial); pastoreo de gramíneas + ramoneo restrictivo de dos horas diarias en Leucaena leucocephala) sobre la producción y composición de la leche y la tasa de crecimiento. Los datos muestran diferencias significativas (P

  16. Comportamento ingestivo de bezerros em pastos de Brachiaria brizantha e Brachiaria decumbens Ingestive behavior of calves on pastures of Brachiaria brizantha and Brachiaria decumbens

    Directory of Open Access Journals (Sweden)

    Anderson de Moura Zanine

    2006-10-01

    Full Text Available Foi conduzido um experimento com o objetivo de avaliar o comportamento de bezerros em pastagens de Brachiaria brizantha e Brachiaria decumbens, no sistema de lotação contínua com taxa de lotação variável. O delineamento experimental foi o inteiramente casualizado, com dois pastos representando os tratamentos, cada um com cinco repetições. O período experimental foi de 30 dias, sendo 20 para adaptação dos animais e 10 para avaliações, que consistiram de três, com duração de 24h cada, em intervalos de cinco dias. Os bezerros pastejaram menos tempo no pasto de Brachiaria brizantha, com tempo de 9,75h, enquanto que o pastejo na Brachiaria decumbens foi de 11,3h. Comportamento diferente foi observado para o tempo de ruminação: 6,8 e 6,4 para os pastos de Brachiaria brizantha e Brachiaria decumbens, respectivamente. O número de bocados por minuto foi menor para a Brachiaria decumbens, 31,15, enquanto que, para a Brachiaria brizantha, os bezerros pastejaram com uma taxa de bocados de 34,91 bocados por minuto. Os bezerros tiveram um ganho de peso médio diário superior no pasto de Brachiaria brizantha (390g dia-1, enquanto que, para a Brachiaria decumbens, o ganho diário foi de 340g dia-1.An experiment was carried out to evaluate the ingestive behavior of calves on Brachiaria brizantha and Brachiaria decumbens pastures, under continuous stocking with variable stocking rate. The experimental design was entirely randomized, with two treatments (two pastures, and five replicates per treatment. The experimental period lasted 30 days, consisting of days 20 for adaptation of animals and 10 for behavioral evaluations that consisted of three periods of 24:00h each, in intervals of five days. The calves spent less time grazing Brachiaria brizantha pasture (9,8h than they did on the Brachiaria decumbens (11,3h. Different rumination times had been observed, 6,8 and 6,4 h for the animals grazing Brachiaria brizantha and Brachiaria decumbens

  17. Acción política y redes de solidaridad étnica entre los indios de Pasto en tiempos de la Independencia.

    Directory of Open Access Journals (Sweden)

    Jairo Gutiérrez Ramos

    2007-06-01

    Full Text Available This article will sketch the antecedents and the context in which Indian peasants from Pasto and its surroundings established the links of ethnic solidarity and strategies of political action that would surface during the anti-republican rebellions that occurred between 1822 and 1825. It starts with the supposition that the peasant mobilization that made these rebellions possible was not led by the traditional elite, but was rather a remarkable expression of the indigenous communities' autonomy and the centrality of their actions. It will examine the experiences that made possible the building of social, ethnic, and political networks whose greatest expression occurred during the explosion of rural violence that shocked southern New Granada and northern Ecuador in the decisive period of the war against Spanish dominance.

  18. Tipo de agresión resultado de un problema y conflicto en la población adolescente de siete instituciones públicas de educación media de San Juan de Pasto

    Directory of Open Access Journals (Sweden)

    Claudia Amanda Chaves

    2015-06-01

    Full Text Available Objetivo: determinar el tipo de agresión que resulta de un problema o conflicto en adolescentes de siete instituciones públicas de educación media de San Juan de Pasto, Nariño, periodo 2013 - 2014. Materiales y Métodos: estudio cuantitativo con diseño descriptivo. La información se obtuvo a través de un cuestionario diseñado y modificado por las investigadoras y autoadministrado por los estudiantes de siete instituciones públicas de educación media jornada de la mañana. La población estuvo conformada por 5537 adolescentes entre las edades de 10 a 17 años, se realizó un muestreo probabilístico aleatorio simple y a través de la afijación proporcional se tomó una muestra de 774 estudiantes. Resultados: se encontró que los lugares con mayor prevalencia donde se presenta el tipo de agresión entre estudiantes son: cerca al colegio, al salir de clase, al interior del aula cuando no está el profesor y por último en pasillos y baños. El tipo de agresión más frecuente es la verbal, que por lo general se origina cuando se habla mal de la persona o por decir palabras ofensivas; las patadas, golpes y peleas entre grupos como tipo de agresión física no reportaron una frecuencia alta entre los adolescentes. Conclusión: el matoneo continúa presente en las instituciones de educación media convirtiéndose en una problemática social que ha sido tratada por diferentes sectores con el fin de encontrar una solución, pero los esfuerzos no están dando resultados; por ello, se debe continuar fortaleciendo el diseño de estrategias e implementación de modelos de convivencia sana orientadas a padres, educadores y estudiantes para la solución de conflictos y comprensión de consecuencias.

  19. Picture Communication in Papua New Guinea.

    Science.gov (United States)

    Cook, Bruce L.

    1980-01-01

    Reports the major findings of a study that investigated the effectiveness of using pictures of different art styles (stick figures, faceless outline drawings, detailed black-and-white, detailed black-and-white with watercolor wash, and black-and-white photographs) with 423 new readers in Papua New Guinea. (JD)

  20. The Papua and New Guinea Herbarium

    NARCIS (Netherlands)

    Womersley, J.S.; Botanist, Forest

    1951-01-01

    A short account of the establishment of the Herbarium of the Department of Forests, Papua and New Guinea appeared on p. 61, no 3, volume I of Flora Malesiana Bulletin. This Herbarium has now been firmly established and is constantly receiving collections of plants from various parts of Papua and New

  1. ECG telemetry in conscious guinea pigs.

    Science.gov (United States)

    Ruppert, Sabine; Vormberge, Thomas; Igl, Bernd-Wolfgang; Hoffmann, Michael

    2016-01-01

    During preclinical drug development, monitoring of the electrocardiogram (ECG) is an important part of cardiac safety assessment. To detect potential pro-arrhythmic liabilities of a drug candidate and for internal decision-making during early stage drug development an in vivo model in small animals with translatability to human cardiac function is required. Over the last years, modifications/improvements regarding animal housing, ECG electrode placement, and data evaluation have been introduced into an established model for ECG recordings using telemetry in conscious, freely moving guinea pigs. Pharmacological validation using selected reference compounds affecting different mechanisms relevant for cardiac electrophysiology (quinidine, flecainide, atenolol, dl-sotalol, dofetilide, nifedipine, moxifloxacin) was conducted and findings were compared with results obtained in telemetered Beagle dogs. Under standardized conditions, reliable ECG data with low variability allowing largely automated evaluation were obtained from the telemetered guinea pig model. The model is sensitive to compounds blocking cardiac sodium channels, hERG K(+) channels and calcium channels, and appears to be even more sensitive to β-blockers as observed in dogs at rest. QT interval correction according to Bazett and Sarma appears to be appropriate methods in conscious guinea pigs. Overall, the telemetered guinea pig is a suitable model for the conduct of early stage preclinical ECG assessment. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. Mesothelium of Reissner's membrane in guinea pigs

    DEFF Research Database (Denmark)

    Qvortrup, K; Rostgaard, Jørgen

    1990-01-01

    The mesothelial cells of Reissner's membrane in guinea pigs were found to be connected by junctional complexes. No cell discontinuities or gaps were observed by scanning or transmission electron microscopy. These results are not in accordance with previous studies. They were achieved by in vivo...

  3. Piraatlus Guinea lahes ja Nigeerias / Eero Tepp

    Index Scriptorium Estoniae

    Tepp, Eero

    2013-01-01

    Guinea lahes lokkava piraatluse põhjused ja tegurid, mis seda soodustavad: seaduselüngad, looduslikud tingimused, ebapädev korrakaitse, poliitiline olustik, kultuuriline vastuvõetavus, majanduslik tasuvus. Sõjalised ja mittesõjalised meetmetest, mida rakendatakse piraatlusevastases võitluses

  4. Arrangement of Renal Arteries in Guinea Pig.

    Science.gov (United States)

    Mazensky, David; Flesarova, Slavka

    2017-03-01

    The aim of this study was to describe origin, localization, and variations of renal arteries in guinea pig. The study was carried out on 26 adult guinea pigs. We prepared corrosion casts of the guinea pig arterial system. Batson's corrosion casting kit no. 17 was used as the casting medium. In 57.7% of specimens, a. renalis dextra was present as a single vessel with different level of its origin from aorta abdominalis. In 38.5% of specimens, two aa. renales dextrae were present with variable origin and arrangement. The presence of three aa. renales dextrae we found in one specimen. In 76.9% of specimens, a. renalis sinistra was present as a single vessel with different level of its origin from aorta abdominalis and variable arrangement. In 23.1% of specimens, we found two aa. renales sinistrae with variable origin and arrangement. The anatomical knowledge of the renal arteries, and its variations are of extreme importance for the surgeon that approaches the retroperitoneal region in several experiments, results of which are extrapolated in human. This is the first work dealing with the description of renal arteries arrangement in guinea pig. Anat Rec, 300:556-559, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  5. Iinformation accessibility and farmers manageriability of guinea ...

    African Journals Online (AJOL)

    The study examined the farmers' access to information and their manageriability of the intervention programme in the study area. To achieve the objective, there was need to determine farmers' information accessibility and manageriability of guinea worm intervention package; then determine farmers satisfaction with the ...

  6. María Teresa Álvarez Hoyos, Elites intelectuales en el sur de Colombia: Pasto, 1904-1930 una generación decisiva, Pasto: Universidad de Nariño, 2007, 552 p

    Directory of Open Access Journals (Sweden)

    Gabriel David Samacá Alonso

    2011-05-01

    Full Text Available La obra en cuestión constituye la tesis doctoral de la profesora Álvarez Hoyos, quien culminó sus estudios en el marco del doctorado interinstitucional en Ciencias de la Educación (RUDE Colombia, estando adscrita como profesora a la Universidad de Nariño. De esta manera, procederemos a resaltar los principales postulados de la obra sobre la configuración de un grupo de intelectuales en el sur de Colombia, procurando compartir con ustedes la lectura de un interesante trabajo sobre la historia cultural y política del país en los albores del siglo pasado. La estructura y estilo de esta reseña corresponde a un ejercicio analítico de lectura realizado para una investigación sobre el Centro de Historia de Santander.

  7. Diagnóstico y propuesta para el perfeccionamiento de la Maestría en Pastos y Forrajes de la EEPF ¨Indio Hatuey¨ Diagnosis and proposal for improving the Master of Science Program in Pastures and Forages of the EEPF «Indio Hatuey»

    Directory of Open Access Journals (Sweden)

    Alicia Ojeda

    2008-12-01

    Full Text Available Con el objetivo de conocer los criterios acerca del contenido de los cursos que integran la Maestría en Pastos y Forrajes, de la EEPF «Indio Hatuey», se desarrolló un proceso de diagnóstico mediante la aplicación de encuestas a egresados y a profesores de la Maestría, así como a profesores e investigadores de centros de educación superior y entidades de ciencia e innovación tecnológica. Los encuestados coincidieron en que es necesario actualizar dicho contenido, acorde con los avances en el sector agropecuario a nivel nacional e internacional, y sugirieron la inclusión de nuevos temas y/o cursos. Los aportes de los miembros del Comité Académico y la consulta de bibliografía permitieron formular una propuesta de nueva estructura y contenido de la Maestría, integrada por un conjunto de conocimientos estrechamente relacionados entre sí: 1 un diplomado básico (Bases científicas para la investigación; un diplomado de formación general (Fundamentos para el desarrollo de una ganadería agroecológica; dos diplomados de especialización, en dependencia del tema de investigación del maestrante: 1 Agronomía de los pastos y forrajes; 2 Sistemas agroecológicos de producción animal. Se concluye que dicha propuesta puede contribuir a la actualización del contenido temático y al perfeccionamiento de la Maestría, y se recomienda a su Comité Académico tener en consideración estos resultados e introducir las modificaciones propuestas en el plan de estudios, con la finalidad de garantizar un mejor desempeño de los egresados en su actividad profesional.With the objective of learning the criteria about the content of the courses that integrate the Master of Science Program in Pastures and Forages, of the EEPF «Indio Hatuey», a diagnosis process was developed, by means of the application of surveys to former students and to professors of the Master program, as well as to professors and researchers of centers of higher education and

  8. El sello de correos, como medio informativo. La antigua Guinea española, en su filatelia

    Directory of Open Access Journals (Sweden)

    Dr. Victoriano Darias

    2001-01-01

    Full Text Available A través de la observación detallada de las colecciones y catálogos filatélicos de un determinado país es posible obtener interesantes consideraciones históricas y periodísticas del mismo. Como ejemplo de ello proponemos en estas líneas el estudio de la faceta filatélica de la Guinea Española, territorio africano discontinuo (islas y zona continental que ocupó España desde 1778, mediante la desafortunada expedición del conde de Argelejo.

  9. Heterogeneity of Opioid Binding Sites in Guinea Pig Spinal Cord

    Science.gov (United States)

    1984-11-30

    MEDICAL CENTER WILFORD HALL AIR FORCE MEDICAL CENTER Title of Thesis: "Heterogeneity of Opioid Binding Sites in Guinea Pig Spinal Cord" Name of...that the use of any copyrighted material in the dissertation manuscript entitled: "Heterogeneity of Opioid Binding Sites in Guinea Pig Spinal Cord...University of the Health Sciences 11 Abstract Title of Thesis: Heterogenity of Opioid Binding Sites In Guinea Pig Spinal Cord Gary Dean Zarr MAJ/ANC

  10. Resultados preliminares del forraje de Morus alba en la alimentación de vacas lecheras

    Directory of Open Access Journals (Sweden)

    M. Milera

    2007-01-01

    Full Text Available El objetivo del presente trabajo consistió en evaluar el potencial del forraje de M. alba con vacas mestizas para la producción de leche. El trabajo se desarrolló en Cuba, la cual se encuentra localizada entre los 19° y 81’ de longitud oeste. La Estación Experimental de Pastos y Forrajes “Indio Hatuey” está ubicada en el municipio de Perico, provincia de Matanzas, sobre un suelo ferralítico rojo, a los 22° 40’ 7’’ de latitud norte y 81° 2’ de longitud oeste, a una altura de 10.91 msnm. La evaluación se hizo con vacas mestizas del cruce Holstein x Cebú, que tenían 54 días de lactancia. El estudio se dividió en dos periodos: en el primero (que duró 53 días se suministró el forraje de morera ad libitum, sin trocear, y se pastoreó de forma restringida en gramíneas mejoradas de secano, sin fertilización; en el segundo periodo, los animales dispusieron de pastoreo de gramíneas mejoradas con un 10% de un área establecida con Leucaena leucocephala y recibieron forraje de morera restringido hasta el 1% del peso vivo; éste tuvo una duración de 87 días. Se determinó la disponibilidad de pasto, la composición bromatológica y el consumo de forraje de Morus alba, así como la producción de leche de las vacas. El forraje de morera presentó altos va- lores de proteína y bajos contenidos de fibra; los consumos en el primer periodo llegaron hasta 2.7% del peso vivo. La producción de leche promedio fue de 10.6 kg/vaca/día durante los 140 días de evaluación y la máxima producción se registró en los primeros 53 días (1 kg/vaca/día. Los resultados demostraron la alta calidad de la morera cuando se utiliza como forraje para la alimentación de vacas mestizas. En las condiciones evaluadas, es posible obtener producciones de 10 litros por animal diariamente, cuando se utiliza el forraje de morera en adición al pasto de especies mejoradas, sin suplementación de concentrados.

  11. Frontera Arqueológica vs. Frontera Etnohistórica: Pastos y Quillacingas en la Arqueología del Sur de Colombia1

    OpenAIRE

    Cárdenas-Arroyo, Felipe

    2014-01-01

    De tantos temas que le competen a la disciplina arqueológica, uno de los más polémicos es el de las fronteras. Desde sus inicios, en Colombia la arqueología se ha interesado por la delimitación de territorios étnicos y de "áreas culturales", términos que casi siempre vinieron a confundirse entre sí. Tal definición de las "áreas culturales" solía implicar un espacio geográfico amplio, casi siempre con diversas características ecológicas que pueden incluir, por ejemplo, el valle cálido de un rí...

  12. Evaluación de alternativas para el tratamiento de lixiviados en el relleno sanitario Antanas del Municipio de San Juan de Pasto en Colombia

    OpenAIRE

    Arango Romero, Paola Andrea

    2013-01-01

    El agua es uno de los recursos naturales más íntimamente unido a la vida, se encuentra de forma abundante en la Tierra, por ello este recurso se ha convertido en tema de discusión a nivel mundial, por la importancia y la necesidad de abogar la gestión sostenible sobre todo de los recursos de agua dulce. En los países en desarrollo, el consumo de agua y la generación de residuos sólidos está en aumento; el tema de la gestión de residuos resulta un problema complejo en el cual se integran conce...

  13. Spontaneous reproductive pathology in female guinea pigs.

    Science.gov (United States)

    Veiga-Parga, Tamara; La Perle, Krista M D; Newman, Shelley J

    2016-11-01

    Reproductive pathology of domestic guinea pigs is underreported to date. To provide a comprehensive review of uterine disease in guinea pigs, we performed a retrospective study of the pathology archives of the University of Tennessee, College of Veterinary Medicine. By histology, 13 of 37 uterine lesions in 23 animals were neoplastic; the other 24 nonneoplastic lesions included cystic endometrial hyperplasia (16 of 24), endometrial hemorrhage (3 of 24), pyometra (2 of 24), polyp (2 of 24), and mucometra (1 of 24). The most common guinea pig uterine neoplasms were uterine leiomyomas (6 of 13), followed by adenomas (3 of 13) and leiomyosarcomas (1 of 13). Other neoplasms included anaplastic tumors of unknown origin (2 of 13) and choriocarcinoma (1 of 13). Both anaplastic tumors and the choriocarcinoma were positive for vimentin. The choriocarcinoma was positive for HSD83B1, indicating a trophoblastic origin and its final diagnosis. All were negative for cytokeratin and smooth muscle. In multiple animals, more than 1 tumor or lesion was reported. Estrogen receptor and progesterone receptor expression was nearly 100% in uterine neoplasms. Nearly all animals for which data were available had cystic rete ovarii (18 of 19); the animal with no cystic rete ovarii had paraovarian cysts. In our study, female pet guinea pigs had a tendency to develop cystic endometrial hyperplasia and uterine neoplasia. Factors for the development of these lesions could be cystic rete ovarii, hormone dysregulation, and/or age. Other factors could contribute to the development of uterine lesions. As in other species, early ovariohysterectomy could decrease the prevalence of uterine lesions. © 2016 The Author(s).

  14. Oil and gas in Papua New Guinea

    International Nuclear Information System (INIS)

    Owen, A.D.; Lattimore, J.C.

    1998-01-01

    This paper documents the current status of oil and gas reserves and production in Papua New Guinea. It then provides an assessment of future prospects for the industry in the context of both the international market for oil and gas and local constraints on the industry's growth. Finally, an estimate of the impact of the industry's development on the economy of PNG is presented. (author)

  15. [Experiences with cage combinations for guinea pigs].

    Science.gov (United States)

    von Zychlinski, J

    1989-01-01

    Special cage units described in 1982 for guinea pigs have been used either as cages for small groups of breeding animals or for caging of growing animals. By using these cages the following advantages have been noted; the cage size can be adapted to number, age and body weight of the animals; aggression and panic are avoided by corners, walls and tunnels; economic use of breeding males by mating with more females.

  16. Elastase-induced emphysema in guinea pigs

    International Nuclear Information System (INIS)

    Loscutoff, S.M.

    1979-01-01

    Pulmonary function changes measured in guinea pigs 4 to 5 wk following intratracheal instillation of crystalline porcine pancreatic elastase resembled comparable changes in humans with moderately severe pulmonary emphysema. Compared with saline-treated controls, elastase-treated animals had increased values for all divisions of lung volume, increased static compliance and prolonged time constants. Since humans with emphysema are especially sensitive to air pollutants, elastase-treated animals may be useful as sensitive animal models in inhalatio toxicology

  17. Penología e indigenismo en la antigua Guinea española

    Directory of Open Access Journals (Sweden)

    Pedro María Belmonte Medina

    1998-01-01

    Full Text Available Este artículo de investigacióm pretende ser un estudio histórico-juridico del derecho penal que los españoles aplicaron a los indígenas de la Guinea española. Partiendo de la base de que el derectio penal guíneano-español se basaba, principalmente, en el reconocimiento de normas indígenas de tipo consuetudinario (derecho indígena, a través de sentencias judiciales y por adaptaciones del derecho metropolitano, usando como vehículo de expresión, así mismo, la jurisprudencia aplicada por los tribunales de raza españoles, se hace un amplio estudio de las penas que los españoles aplicaron a los indígenas, dividiendo la obra en dos parles: derecho para indígenas y derecho indígena.This work tries to be a historic-juridical study about penal law that spanish people applied to natives fron the spanish guinea. From the point of view that this law was, mainly, based on the knowledge of the natives rules about habits and native law, through sentences and the adaptation of the metropolitan law, using as mass media, the jurisprudence applied by the spanish courts, a huge study, is made about the punishments spanish people applied to natives. The study is divided in two parts: native law and law for natives.

  18. Variabilidade espacial e temporal da vegetação em pastos de capim braquiária diferidos Spatial and temporal variability of vegetation on deferred signalgrass pastures

    Directory of Open Access Journals (Sweden)

    Manoel Eduardo Rozalino Santos

    2010-04-01

    Full Text Available Avaliou-se a variabilidade espacial e temporal de características descritoras da condição de pastos diferidos de Brachiaria decumbens cv. Basilisk (capim-braquiária. Os tratamentos consistiram de combinações dos períodos de diferimento da pastagem (73, 103, 131 e 163 dias com os períodos de pastejo (29, 57 e 85 dias. Utilizou-se esquema de parcelas subdivididas e delineamento em blocos casualizados com duas repetições. Foi determinada a dispersão dos valores de altura do pasto, altura da planta estendida e do índice de tombamento do pasto. A variabilidade espacial da altura do pasto aumentou de forma linear com o período de diferimento, porém não foi influenciada pelo período de pastejo. O coeficiente de variação da altura da planta estendida diminuiu linearmente em pastos submetidos aos maiores períodos de diferimento e não foi afetado pelo período de pastejo. A variabilidade do índice de tombamento, no entanto, apresentou resposta quadrática ao período de diferimento, com ponto de máximo correspondente ao coeficiente de variação de 38,25% aos 130 dias. Em pastagens diferidas por curto período (73 dias, ocorreu variação negativa do coeficiente de variação durante o período de pastejo. Pastos de capimbraquiária sob diferimento por longos períodos possuem maior variabilidade da altura do pasto e menor heterogeneidade da altura da planta estendida. Além do efeito temporal, ocorre grande variabilidade espacial nas pastagens de capim-braquiária diferidas.It was evaluated spatial and temporal variability of status descriptive characters of Brachiaria decumbens (signalgrass cv. Basilisk deferred pastures. Treatments consisted of combinations of pasture deferring periods (73, 103, 131 and 163 days with grazing periods (29, 57 and 85 days. Randomized block design with two repetitions and subdivided plots was used. It was determined the dispersion of pasture height (PH, stretched plant height (SPH and falling index

  19. An annotated ornithological bibliography of Guinea-Bissau

    NARCIS (Netherlands)

    Hazevoet, C.J.

    1995-01-01

    Guinea-Bissau, a former Portuguese colony, is a small country (36,125 km²) in West Africa bordered by Senegal in the north and Guinea (Conakry) in the east and south. Little is known about the country’s ornithology and comparatively few papers on its birds have been published. The following is an

  20. CHARACTERIZATION OF MUSCARINIC RECEPTORS IN GUINEA-PIG UTERUS

    NARCIS (Netherlands)

    DOODS, HN; WILLIM, KD; BODDEKE, HWGM; ENTZEROTH, M

    1993-01-01

    To characterize the muscarinic receptor present in guinea-pig uterus smooth muscle the affinities of a series of 27 muscarinic receptor antagonists for M1 (rat cortex), M2 (rat heart), M3 (rat submandibular gland), m4 (transfected in CHO cells) and muscarinic binding sites in guinea-pig uterus

  1. Heterogeneous infectiousness in guinea pigs experimentally infected with Trypanosoma cruzi.

    Science.gov (United States)

    Castillo-Neyra, Ricardo; Borrini Mayorí, Katty; Salazar Sánchez, Renzo; Ancca Suarez, Jenny; Xie, Sherrie; Náquira Velarde, Cesar; Levy, Michael Z

    2016-02-01

    Guinea pigs are important reservoirs of Trypanosoma cruzi, the causative parasite of Chagas disease, and in the Southern Cone of South America, transmission is mediated mainly by the vector Triatoma infestans. Interestingly, colonies of Triatoma infestans captured from guinea pig corrals sporadically have infection prevalence rates above 80%. Such high values are not consistent with the relatively short 7-8 week parasitemic period that has been reported for guinea pigs in the literature. We experimentally measured the infectious periods of a group of T. cruzi-infected guinea pigs by performing xenodiagnosis and direct microscopy each week for one year. Another group of infected guinea pigs received only direct microscopy to control for the effect that inoculation by triatomine saliva may have on parasitemia in the host. We observed infectious periods longer than those previously reported in a number of guinea pigs from both the xenodiagnosis and control groups. While some guinea pigs were infectious for a short time, other "super-shedders" were parasitemic up to 22 weeks after infection, and/or positive by xenodiagnosis for a year after infection. This heterogeneity in infectiousness has strong implications for T. cruzi transmission dynamics and control, as super-shedder guinea pigs may play a disproportionate role in pathogen spread. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  2. Epidemiological evidence of listeriosis in guinea pigs fed with ...

    African Journals Online (AJOL)

    Listeria monocytogenes is a bacterium that infects livestock and humans. We report the first outbreak of invasive listeriosis caused by L. monocytogenes in a guinea pig breeding colony. Eighty to 100% mortality rate was recorded in the colony of 80 guinea pigs within four weeks outbreak. On epidemiologic investigation ...

  3. Ebola Virus Imported from Guinea to Senegal, 2014.

    Science.gov (United States)

    Ka, Daye; Fall, Gamou; Diallo, Viviane Cissé; Faye, Ousmane; Fortes, Louise Deguenonvo; Faye, Oumar; Bah, Elhadji Ibrahim; Diallo, Kadia Mbaye; Balique, Fanny; Ndour, Cheikh Tidiane; Seydi, Moussa; Sall, Amadou Alpha

    2017-06-01

    In March 2014, the World Health Organization declared an outbreak of Ebola virus disease in Guinea. In August 2014, a case caused by virus imported from Guinea occurred in Senegal, most likely resulting from nonsecure funerals and travel. Preparedness and surveillance in Senegal probably prevented secondary cases.

  4. Quality Attributes Of Two Species Of Indigenous Guinea Fowl Meat ...

    African Journals Online (AJOL)

    As the duration of storage of the guinea fowl meats increased percentage of weight loss, cooking loss, pH, and tenderness scores increased (P < 0.05). Prepackaging or salting of guinea fowl meats reduced (P< 0.05) percentages of weight loss, cooking loss and increased (P < 0.05) pH, water holding Capacity and sensory ...

  5. Piper (Piperaceae) in New Guinea: the climbing species

    NARCIS (Netherlands)

    Gardner, R.O.

    2012-01-01

    Sixteen climbing Piper species are accepted for New Guinea. The three endemics, P. arfakianum, P. subcanirameum and P. versteegii, are fully described. Eight taxa of unclear circumscription are noted. A new variety of P. macropiper, endemic to Morobe Province of Papua New Guinea, is described. The

  6. Transplacental absorption of 238Pu in rats and guinea pigs

    International Nuclear Information System (INIS)

    Sullivan, M.F.

    1980-01-01

    Pregnant rats and guinea pigs were injected intravenously with 238 Pu citrate to determine if the potential for in utero accumulation of 238 Pu by these two species is related to the stage of development at which immunity is gained. Although guinea pigs retained more 238 Pu after birth than rats, the difference was not significant

  7. Crossed Looks: Globalisations and Curriculum in Guinea-Bissau

    Science.gov (United States)

    da Silva, Rui; dos Santos, Júlio Gonçalves; Pacheco, José Augusto

    2015-01-01

    This article focuses on education in Guinea-Bissau in the context of globalisations, examining the concept of globalisation and its relation to education and the curriculum. It focuses on the relatively neglected area of national education policies in Guinea-Bissau, comparing differences and common points of interference/influence of multilateral…

  8. Briti eliit üritas kukutada Ekvatoriaal-Guinea valitsust

    Index Scriptorium Estoniae

    2004-01-01

    Ekvatoriaal-Guineas ja Zimbabwes on vahistatud kümneid inimesi, keda süüdistatakse riigipöördekatses Ekvatoriaal-Guineas. Suurbritannia endise peaministri Margaret Thatcher'i poega Mark Thatcher'it süüdistatakse riigipöördekatse rahastamisele kaasaaitamises

  9. A 2-D guinea pig lung proteome map

    Science.gov (United States)

    Guinea pigs represent an important model for a number of infectious and non-infectious pulmonary diseases. The guinea pig genome has recently been sequenced to full coverage, opening up new research avenues using genomics, transcriptomics and proteomics techniques in this species. In order to furth...

  10. RELATIONSHIP BETWEEN BEHAVIORAL AND AUTONOMIC THERMOREGULATION IN THE GUINEA PIG

    Science.gov (United States)

    The study was conducted to correlate the preferred thermal environment of the unrestrained guinea pig with the activity of its thermoregulatory effectors when maintained under a wide range of ambient temperatures (Ta). Eight male guinea pigs were used in a series of experiments o...

  11. The Guinea Pigs of a Problem-Based Learning Curriculum

    Science.gov (United States)

    Reddy, Sarasvathie; McKenna, Sioux

    2016-01-01

    Participants in a study on learning the clinical aspects of medicine in a problem-based learning (PBL) curriculum repeatedly referred to themselves as "Guinea pigs" at the mercy of a curriculum experiment. This article interrogates and problematises the "Guinea pig" identity ascribed to and assumed by the first cohort of…

  12. An Analysis Of Guinea Worm Infection On Yam Productivity In ...

    African Journals Online (AJOL)

    Guinea worm infection has been reported to be one of the external diseconomies factors that limit agricultural productivity of the rural farm households. The study examined the determinants of farmers' vulnerability to guineaworm infection and the effect of guinea worm infection on yam yield in the study area. A stratified ...

  13. Confinement lowers fertility rate of helmeted guinea fowl ( Numida ...

    African Journals Online (AJOL)

    ... common game bird in Africa and there have been efforts to domesticate it for use as a source of human food. An important obstacle in successful domestication of guinea fowl is their low fertility rate.We studied the effects of semi-confinement on the fertility rates of helmeted guinea fowl by comparing egg fertility, hatch rate ...

  14. Extraction and application of dyestuffs from the leaves of guinea ...

    African Journals Online (AJOL)

    This research work is aimed at assessing the dyeing and colouring potentials of ethanol extracts from the leaves of guinea corn and the skin of onion. It is intended to apply the purified dye extracts on textile, drink and food. The leaves of guinea corn (Sorghum bicolor) and the skin of onion (Allium cepa) were collected, ...

  15. First records in Guinea Bissau of Adamawa Turtle Dove Streptopelia ...

    African Journals Online (AJOL)

    There are no confirmed records of the Adamawa Turtle Dove Streptopelia hypopyrrha in north-eastern Guinea Bissau and there is very little information available on the biology of the species. Eight individuals of the Adamawa Turtle Dove were identified from the game bags of sport hunters in north-eastern Guinea Bissau, ...

  16. Efecto de dos inhibidores de la nitrificación sobre la concentración de nitratos en pasto elefante (Pennisetum purpureum Schum.

    Directory of Open Access Journals (Sweden)

    Sánchez G. Hugo

    1986-09-01

    Full Text Available Se compararon dos inhibidores de la nitrificación (Sulfatiazol, N- Serve y las tendencias de la conversión de amonio a nitrato en el suelo y la concentración de este ión en el pasto según la fuente (Urea, Nitrón 26 y las dosis de nitrógeno (100, 200 kg/ha. Las concentraciones de nitratos en los suelos y en el tejido vegetal tendieron a incrementarse a medida que aumentaban los niveles de fertilización. La efectividad de los inhibidores se encuentra determinada por los niveles de fertilización y, en algunos casos, por las condiciones medio ambientales presentes.It was compared the grade of inhibition of two inhibitors products of the nitrification (Sulfatiazol, N - Serve and the tendencies on the conversion of ammonium into nitrate in the ground and the concentration of this ion on Pennisetum purpureum Schum. Depending on the source (urea, Nitrón 26 and the quantity of nitrogen (100, 200 kq/ha. The concentrations of nitrates on the grounds and the vegetal tissue stretched to intensity at the same time as were increase the levels of nitrogenous. The effectiveness of the inhibitors is determined for the employed levels and in some cases for the present environment conditions.

  17. Gastric volvulus in guinea pigs: comparison with other species.

    Science.gov (United States)

    Dudley, Emily S; Boivin, Gregory P

    2011-07-01

    Gastric volvulus has been documented in several species of animals and is associated with high morbidity and mortality. We report 2 cases of gastric volvulus in guinea pigs that died without detection of prior clinical signs. Both guinea pigs were adult female guinea pigs in a breeding colony and had given birth to multiple litters; one was pregnant at the time of death. Gastric rotations of 540° and 360° were identified at necropsy examination. These cases include the first known report of gastric rotation greater than 360° in any species. Although gastric volvulus has been reported to occur in guinea pigs, little is known about its risk factors, etiology, and pathogenesis. We conducted a literature review to compare gastric volvulus between guinea pigs and other species. Copyright 2011 by the American Association for Laboratory Animal Science

  18. Unilateral flank ovariohysterectomy in guinea pigs (Cavia porcellus).

    Science.gov (United States)

    Rozanska, D; Rozanski, P; Orzelski, M; Chlebicka, N; Putowska, K

    2016-11-01

    To describe a simple, minimally invasive method of ovariohysterectomy via a unilateral flank approach in guinea pigs, for use in routine desexing of healthy female guinea pigs or treatment of ovarian cysts. The subjects of this retrospective study were 41 client-owned guinea pigs submitted for routine desexing or treatment of ovarian cysts. They included 16 healthy female guinea pigs aged 8-12 months (Group 1), and 15 females aged from 9 months to 3 years (Group 2), and 10 females aged from 3 to 7 years (Group 3) with different-sized ovarian cysts. Prior to surgery, the animals received clinical examination, blood testing (complete blood count and serum biochemistry profile) and examination of the abdomen using ultrasonography, to assess the condition of the reproductive tract and ensure the guinea pigs were fit for surgery. Ovariohysterectomy was performed via a unilateral flank incision made close to the erector spinae muscle starting approximately 1 cm caudal to the last rib. Both ovaries, uterine horns, and the uterine cervix were localised, ligated, and dissected through this unilateral retroperitoneal incision. Ovariohysterectomy was successfully completed via a single flank incision in 38/41 (93%) guinea pigs. Three guinea pigs with ovarian cysts from Group 3, which were >6 years old died during surgery due to circulatory and respiratory failure under anaesthesia. In the remaining 38 cases, surgery proceeded without complications. A further two guinea pigs from Group 3 were reluctant to move or eat for the first 3 days after surgery but recovered after provision of supportive care. All 38 animals fully recovered and wound healing was normal. This is the first report of ovariohysterectomy via a unilateral flank incision in guinea pigs. This approach is a simple, minimally invasive and safe alternative to the midline or bilateral flank approaches currently used for surgery of the reproductive tract in guinea pigs.

  19. Taxonomy Icon Data: Guinea baboon [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Guinea baboon Papio papio Chordata/Vertebrata/Mammalia/Theria/Eutheria/Primate Papio_papio_L.png Papio_papi...o_NL.png Papio_papio_S.png Papio_papio_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Papio+papi...o&t=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Papio+papio&t=NL http://bioscien...cedbc.jp/taxonomy_icon/icon.cgi?i=Papio+papio&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Papio+papio&t=NS ...

  20. Comparison of Human and Guinea Pig Acetylcholinesterase Sequences and Rates of Oxime-Assisted Reactivation

    Science.gov (United States)

    2010-01-01

    of appropriate animal model systems. For OP poisoning, the guinea pig (Cavia porcellus) is a commonly used animal model because guinea pigs more...endogenous bioscavenger in vivo. Although guinea pigs historically have been used to test OP poisoning therapies, it has been found recently that guinea pig AChE...transcribed mRNA encoding guinea pig AChE, amplified the resulting cDNA, and sequenced this product. The nucleotide and deduced amino acid sequences of

  1. Blood profiles in unanesthetized and anesthetized guinea pigs (Cavia porcellus).

    Science.gov (United States)

    Williams, Wendy R; Johnston, Matthew S; Higgins, Sarah; Izzo, Angelo A; Kendall, Lon V

    2016-01-01

    The guinea pig is a common animal model that is used in biomedical research to study a variety of systems, including hormonal and immunological responses, pulmonary physiology, corticosteroid response and others. However, because guinea pigs are evolutionarily a prey species, they do not readily show behavioral signs of disease, which can make it difficult to detect illness in a laboratory setting. Minimally invasive blood tests, such as complete blood counts and plasma biochemistry assays, are useful in both human and veterinary medicine as an initial diagnostic technique to rule in or rule out systemic illness. In guinea pigs, phlebotomy for such tests often requires that the animals be anesthetized first. The authors evaluated hematological and plasma biochemical effects of two anesthetic agents that are commonly used with guinea pigs in a research setting: isoflurane and a combination of ketamine and xylazine. Hematological and plasma biochemical parameters were significantly different when guinea pigs were under either anesthetic, compared to when they were unanesthetized. Plasma proteins, liver enzymes, white blood cells and red blood cells appeared to be significantly altered by both anesthetics, and hematological and plasma biochemical differences were greater when guinea pigs were anesthetized with the combination of ketamine and xylazine than when they were anesthetized with isoflurane. Overall these results indicate that both anesthetics can significantly influence hematological and plasma biochemical parameters in guinea pigs.

  2. Functional analysis of guinea pig β1-adrenoceptor.

    Science.gov (United States)

    Tanaka, Yoshio; Takahashi, Hiromi; Shibata, Sayuri; Namiki, Kana; Kimura, Sadao; Koike, Katsuo; Kasuya, Yoshitoshi

    2011-12-01

    Although similarity of pharmacological responses to certain stimuli between guinea pigs and humans has been reported, this has been poorly defined by a molecular biological approach. In this study, we cloned the gene of guinea pig ?1-adrenoceptor (ADRB1). The deduced amino acid sequence of guinea pig ADRB1 (467-aa) showed 91% and 92% identity with the human and rat ADRB1 sequences, respectively. Using HEK293T cells expressing guinea pig, human and rat ADRB1s independently, we elucidated the functional characteristics of each ADRB1. The ligand-binding profiles and the concentration-response relationships for isoprenaline-induced cyclic adenosine monophosphate (cAMP) production were similar among the three ADRB1s. Isoprenaline also induced phosphorylation of extracellular-signal related kinases (ERK) through ADRB1s in a concentration-dependent manner. The minimum effective concentration of isoprenaline for phosphorylation of ERK, through guinea pig ADRB1 was the same as through human ADRB1, but markedly lower than that of through rat ADRB1. ERK phosphorylation through guinea pig ADRB1 was sensitive to pertussis toxin, a dominant-negative ras and PD98059, indicating that a G(i)-mediated pathway is involved in the ADRB1/ERK signaling loop. These results suggest that the G(i)-coupling efficacy of guinea pig and human ADRB1s may be higher than that of rat ADRB1.

  3. Biosynthesis of plasmenylcholine in guinea pig heart

    International Nuclear Information System (INIS)

    Wientzek, M.; Choy, P.C.

    1986-01-01

    In some mammalian hearts, up to 40% of the choline phosphoglyceride (CPG) exists as plasmenylcholine (1-alkenyl-2-acyl-glycero-3-phosphocholine). Although the majority of diacylphosphatidylcholine (PC) in mammalian hearts is synthesized from choline via the CDP-choline pathway, the formation of plasmenylcholine from choline was not known. In this study, they investigated the biosynthesis of plasmenyl-choline in the isolated guinea pig heart by perfusion with [ 3 H]choline. Labelled choline containing metabolites and labelled plasmenylcholine were isolated and determined at different perfusion time points. Significant amounts of labelling were found only in choline, phosphocholine, CDP-choline, plasmenyl-choline and PC. In addition, a precursor-product relationship was observed between the labelling of CDP-choline and plasmenylcholine. Such a relationship was not observed between choline and plasmenylcholine. Hence, they postulate that the incorporation of choline into plasmenylcholine is via the CDP-choline pathway and not via base exchange. The ability to condense 1-alkenyl-2-acyl-glycerol with CDP-choline was also demonstrated in vitro with guinea pig heart microsomes

  4. APLICACIONES DE LA TELEDETECCIÓN AL LEVANTAMIENTO DEL USO DE LA TIERRA EN LA ZONA CAFETALERA NORTE DE LA CIUDAD DE ALAJUELA, COSTA RICA

    OpenAIRE

    Hernández Z., Eduardo

    2011-01-01

    El presente es un trabajo de aplicación de la teledetección con el apoyo de la cartografía topográfica y de las fotografías aéreas para el levantamiento del uso del suelo en la zona cafetalera norte de la ciudad de Alajuela, Costa Rica.  La zona de estudio está situada en el Valle Central de Costa Rica.  Se trata de una zona agrícola destinada al cultivo del café, de la caña de azúcar, de pastos extensivos e intensivos y a la horticultura.  Las partes altas de montañas y cañones de ríos son c...

  5. Absorption of cadmium in the newborn and juvenile guinea pig

    International Nuclear Information System (INIS)

    Sasser, L.B.; Jarboe, G.E.

    1978-01-01

    The accepted premise that absorption is enhanced in the newborn animal is based primarily on studies using the rat, which has a relatively immature gastrointestinal system at birth. This study was conducted to determine if enhanced absorption of cadmium also occurs in the guinea pig, a species that is more precocious at birth than the rat. Newborn guinea pigs were administered a single oral dose of 5 μCi of /sup 115m/Cd (0.25 mCi of /sup 115m/Cd per milligram of cadmium) between 8 and 24 hr of age and killed at intervals between 1 and 14 days after the dosing. Juvenile guinea pigs were similarly dosed with 20 μCi of /sup 115m/Cd and killed 1 to 5 days later. Guinea pigs dosed on the day of birth absorbed approximately 6% of the dose by 1 day after the dosing and nearly 25% of the dose by 11 days. This is a cumulative absorption five times that found in rats dosed at 24 hr and two times that of rats dosed at 2 hr. The residence time of cadmium in the gastrointestinal tract was longer in newborn guinea pigs than in juvenile guinea pigs but was still significantly less than that in the newborn rat. We concluded that the absorption of cadmium from the gastrointestinal tract was greater in newborn guinea pigs than in rats, even though the residence time of cadmium in the intestinal tract was less in guinea pigs than in rats and the maturity at birth of the guinea pig is greater than that of the rat. The bases of these differences were not readily apparent

  6. Peces del Noroeste del Ecuador

    OpenAIRE

    Barriga, Ramiro

    1994-01-01

    La ictiofauna del occidente del Ecuador es poco conocida. Los peces del noroccidente son diferentes a los del suroccidente del Ecuador. 34 familias y 82 especies fueron colectadas que equivale al 11 % de las especies de peces continentales registradas en el Ecuador. Icteogeográficamente se sabe que la costa ecuatoriana posee dos provincias: la del Pacifico Norte y la del Guayas, se determinó que el límite de las dos provincias es el río Santiago ya que las especies del mencionado río so...

  7. Gastric Volvulus in Guinea Pigs: Comparison with Other Species

    OpenAIRE

    Dudley, Emily S; Boivin, Gregory P

    2011-01-01

    Gastric volvulus has been documented in several species of animals and is associated with high morbidity and mortality. We report 2 cases of gastric volvulus in guinea pigs that died without detection of prior clinical signs. Both guinea pigs were adult female guinea pigs in a breeding colony and had given birth to multiple litters; one was pregnant at the time of death. Gastric rotations of 540° and 360° were identified at necropsy examination. These cases include the first known report of g...

  8. Salmonella arizonaeInfection In A Guinea Pig Breeding Unit

    OpenAIRE

    GÜREL, Aydın; AYYILDIZ, Gülbin

    1998-01-01

    Salmonella arizonae infection was detected in guinea pigs kept for breeding. Two months old 50 quinea pigs were died in the breedig unit. Three death and 2 live guinea pigs were brought to Veterinary Faculty of İstanbul. The live 2 were euthanasied and necropcy was performed on all guinea pigs. The fluid about 50 cc. in the abdomen, the necrosis and pseudomembranes in the liver and spleen were observed. On histopathology, the foci of various coagulative necrosis were seen in the liver. The...

  9. Dinámica del cadmio en suelos cultivados con papa en Nariño, Colombia.

    Directory of Open Access Journals (Sweden)

    Liliana Insuasty B

    2008-01-01

    Full Text Available Se evaluó la fijación y movilidad de cadmio en suelos dedicados al cultivo de papa en Túquerres, Pasto y Guachucal. Se tomaron muestras de suelos no disturbadas en cilindros de PVC, las cuales, luego de aplicaciones fraccionadas de cadmio con dosis de 0, 50,100 y 150 ppm, se incubaron a capacidad de campo durante cuatro meses. En estos suelos los contenidos de cadmio total e intercambiable no sobrepasaron los límites permisibles establecidos por Organización Mundial de la Salud-Organización de las Naciones Unidas para la Agricultura y la Alimentación (OMS-FAO; no obstante, la mayor cantidad del elemento se localizó en la parte superior de los núcleos, lo cual indica baja movilidad del elemento en el perfil.

  10. AVALIAÇÃO DAS CARACTERÍSTICAS DE CARCAÇA DE NOVILHOS NELORE SUPLEMENTADOS A PASTO NA ESTAÇÃO CHUVOSA

    Directory of Open Access Journals (Sweden)

    Carlos Stuart Coronel Palma

    2006-10-01

    Full Text Available A base de sustentação da pecuária de corte no Brasil são as pastagens, implantadas em aproximadamente 171milhões de hectares. Das 35,7 milhões de cabeças abatidas anualmente no Brasil, apenas 1,5 milhões são terminadas em regime de confinamento, o restante, cerca de 34,2 milhões de cabeças, é mantido em regime de pasto. Na última década, os criadores brasileiros de gado de corte, utilizando-se de técnicas de manejo e alimentação, têm procurado produzir bovinos jovens para o abate, alcançando não só maior produtividade e qualidade de carcaça, como também retorno mais rápido dos investimentos e melhor remuneração. Considerando a necessidade do aumento da eficiência produtiva para viabilização comercial da atividade e a importância da nutrição nesse processo, uma avaliação de alternativas de suplementação torna-se necessária. Assim, com este experimento avaliaram-se os efeitos da suplementação mineral e suplementação completa (mineral, protéica, energética e vitamínica sobre as características de carcaça de novilhos Nelore, terminados a pasto, na estaçãochuvosa. Durante 156 dias, divididos em cinco períodos, foram avaliados 60 novilhos, divididos em três lotes de 20animais, com média de 292,3kg (±19,2 e 27 meses (±2,6 deidade no início do experimento e peso final dos animais de411,5kg (±24,9 de peso vivo aos 32 meses (±2,6, em pastagem de Brachiaria bryzantha cv. Marandu. Foram utilizados três tratamentos: suplemento mineral, suplemento com-pleto e suplemento completo servido em duas porções, uma com os sais minerais e outra com os demais nutrientes. Não houve efeito dos tratamentos (P>0,05 sobre o rendimento de carcaça, área do lombo, espessura de gordura, redução do peso da carcaça no resfriamento e composição da carcaça em traseiro, dianteiro e ponta-de-agulha. PALAVRAS-CHAVE: Bovinos de corte, carcaça, nutrição, pastagem, suplementação.

  11. Comparação de métodos de medida indireta de massa de forragem em pasto de azevém anual (Lolium multiflorum Lam.

    Directory of Open Access Journals (Sweden)

    Cauduro Guilherme Fernandes

    2006-01-01

    Full Text Available Este trabalho foi realizado de maio a outubro de 2003, na Estação Experimental da Universidade Federal do Rio Grande do Sul (EEA-UFRGS, tendo como objetivo avaliar e comparar a precisão do disco medidor de forragem, do bastão graduado e do medidor de capacitância em predizer a massa de forragem de pastagens de azevém anual. O trabalho constou de dois experimentos: o experimento 1 no qual foram avaliados os três métodos numa situação em que o azevém apresentava-se em fase de estabelecimento num delineamento completamente casualizado, com 32 repetições e três tratamentos; e o experimento 2, no qual foi avaliada a massa de forragem pré e pós-pastejo pelo bastão graduado e pelo disco medidor em potreiros sob lotação rotacionada. O delineamento experimental do experimento 2 foi o de blocos completamente casualizados, com quatro repetições; os tratamentos constaram de duas intensidades de pastejo (baixa e moderada definidas por ofertas de forragem de 2,5 ou 5,0 vezes o potencial de consumo dos cordeiros que foram utilizados. No experimento 1, o bastão graduado apresentou o melhor coeficiente de determinação com a massa de forragem (r²=0,65. No experimento 2, os melhores coeficientes de determinação foram obtidos quando o pasto, sob lotação rotacionada, foi avaliado com o bastão graduado.

  12. Evaluación de la zeolita natural utilizada como una tecnología productiva y limpia, aplicada al pasto Maralfalfa (Pennisetum sp) como un complemento en el uso de los fertilizantes nitrogenados

    OpenAIRE

    Pérez-Marín, Manuel Francisco

    2014-01-01

    Proyecto de Graduación (Maestría en Gestión de Recursos Naturales y Tecnologías de Producción) Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Agroforestal, 2014. La necesidad de buscar alternativas para el aprovechamiento de fertilizantes en pastos de alta calidad como Maralfalfa (Pennisetum sp), lleva a investigar a la zeolita a modo de tecnología complementaria al programa de fertilización. Países como México, Colombia, Ecuador y Bolivia han utilizado la zeolita de tal forma ...

  13. Estimación de la demanda de semillas forrajeras mejoradas de pasto miel (Paspalum dilatatum) y agropiro criollo (Elymus scabrifolus) para la producción pecuaria en la Argentina

    OpenAIRE

    Pérez Camargo, Gladys M.; Schrauf, Gustavo Enrique; Ostrowski, Bernardo

    2005-01-01

    p.111-120 Se estudió la posibilidad de éxito de nuevos cultivares de Pasto miel (Paspalum dilatatum) y Agropyro criollo (Elymus scabrifolius) en la Argentina. Se realizó un análisis de demanda potencial, considerando la superficie de pasturas naturales que poseen las provincias agroecológicamente más aptas para ambas especies, estimándose la magnitud y tasa de renovación de praderas artificiales. Con esta información se plantearon cuatro escenarios con distintos porcentajes de implantación...

  14. Analyzing Protein Changes in Guinea Pig Tissue Lysates Using Non-guinea Pig Specific Antibodies: Procedures for Western Blotting and Examples Using 16 Individual Antibodies for Common CNS Proteins

    National Research Council Canada - National Science Library

    Johnson, Erik A; Daugherty, Kelly S

    2006-01-01

    ... behavioral and protein changes due to the absence of guinea pig-specific antibodies. We have developed a procedure to determine the specificity of commercially available, non-guinea pig-specific antibodies in guinea pig lysates...

  15. Effects of ultraviolet irradiation on skin of guinea pig

    International Nuclear Information System (INIS)

    Zhang Wenwen; Chen Qiang; Li Peng; Ling Ling; Lin Xiaochen; Ren Shuping; Liu Yajuan; Li Yun

    2008-01-01

    Objective: To explore the adverse effects of ultraviolet B (UV-B) on the skin of guinea pig. Methods: Guinea pig skin was irradiated with UV-B, the skin changes in external appearance, pathology, and the contents of OH and O 2 - produced in the skin were determined to study the adverse effects of UV-B on the guinea pig skin. Results: UV-B caused red swelling and desquamation of skin, with the increasing of the UV-B irradiation, the cells in stratum spinosum began to proliferate vigorously, the MDA and ROS contents in UVB radiation group were significantly higher than those in control group (P<0.05). Conclusion: UV-B can cause injury to guinea pig skin and has the potential to produce skin cancer. (authors)

  16. (MSV) resistant maize varieties for adaptation to a southern guinea

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-05

    Oct 5, 2009 ... African Journal of Biotechnology Vol. 8 (19), pp. ... the development of superior maize varieties for the southern guinea savanna ecology. Key words: Zea ... tional agricultural research systems have succeeded in converting ...

  17. Non-terminal blood sampling techniques in Guinea pigs

    DEFF Research Database (Denmark)

    Birck, Malene Muusfeldt; Tveden-Nyborg, Pernille; Lindblad, Maiken Marie

    2014-01-01

    Guinea pigs possess several biological similarities to humans and are validated experimental animal models(1-3). However, the use of guinea pigs currently represents a relatively narrow area of research and descriptive data on specific methodology is correspondingly scarce. The anatomical features...... of guinea pigs are slightly different from other rodent models, hence modulation of sampling techniques to accommodate for species-specific differences, e.g., compared to mice and rats, are necessary to obtain sufficient and high quality samples. As both long and short term in vivo studies often require...... repeated blood sampling the choice of technique should be well considered in order to reduce stress and discomfort in the animals but also to ensure survival as well as compliance with requirements of sample size and accessibility. Venous blood samples can be obtained at a number of sites in guinea pigs e...

  18. South Pacific Nuclear Free Zone Treaty; Tratado Sobre La Zona Desnuclearizada Del Pacifico Sur

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1986-02-15

    On 6 August 1985 the South Pacific Forum, a body comprising the independent and self-governing countries of the South Pacific (Australia, the Cook Islands, Fiji, Kiribati, Nauru, New Zealand, Niue, Papua New Guinea, the Solomon Islands, Tonga, Tuvalu, Vanuatu and Western Samoa), endorsed the text of the South Pacific Nuclear Free Zone Treaty and opened it for signature [Spanish] El 6 de agosto de 1985 el Foro del Pacifico Sur, organo compuesto por los paises independientes y autonomos del Pacifico Sur (Australia, Fiji, Islas Cook, Islas Salomon, Kiribati, Nauru, Niue, Nueva Zelandia, Papua Nueva Guinea, Samoa Occidental, Tonga, Tuvalu, y Vanuatu), aprobo el texto del Tratado sobre la Zona Desnuclearizada del Pacifico-Sur y lo abrio a la firma.

  19. Shortage of vaccines during a yellow fever outbreak in Guinea.

    OpenAIRE

    Nathan, N; Barry, M; Van Herp, M; Zeller, H

    2001-01-01

    A yellow fever epidemic erupted in Guinea in September, 2000. From Sept 4, 2000, to Jan 7, 2001, 688 instances of the disease and 225 deaths were reported. The diagnosis was laboratory confirmed by IgM detection in more than 40 patients. A mass vaccination campaign was limited by insufficient international stocks. After the epidemic in Guinea, the International Coordinating Group on Vaccine Provision for Epidemic Meningitis Control decided that 2 million doses of 17D yellow fever vaccine, bei...

  20. Spleens and holoendemic malaria in West New Guinea.

    Science.gov (United States)

    METSELAAR, D

    1956-01-01

    The author describes the results obtained in recent malaria surveys in West New Guinea, where what is essentially holoendemic malaria prevails. However, the spleen-rate in adults differs markedly from what is regarded as normal under holoendemic conditions according to the definition put forward at the Malaria Conference in Equatorial Africa in 1950. The author therefore concludes that that definition is not properly applicable to New Guinea.

  1. Peat in the mountains of New Guinea

    Directory of Open Access Journals (Sweden)

    G.S. Hope

    2015-11-01

    Full Text Available Peatlands are common in montane areas above 1,000 m in New Guinea and become extensive above 3,000 m in the subalpine zone. In the montane mires, swamp forests and grass or sedge fens predominate on swampy valley bottoms. These mires may be 4–8 m in depth and up to 30,000 years in age. In Papua New Guinea (PNG there is about 2,250 km2 of montane peatland, and Papua Province (the Indonesian western half of the island probably contains much more. Above 3,000 m, peat soils form under blanket bog on slopes as well as on valley floors. Vegetation types include cushion bog, grass bog and sedge fen. Typical peat depths are 0.5‒1 m on slopes, but valley floors and hollows contain up to 10 m of peat. The estimated total extent of mountain peatland is 14,800 km2 with 5,965 km2 in PNG and about 8,800 km2 in Papua Province. The stratigraphy, age structure and vegetation histories of 45 peatland or organic limnic sites above 750 m have been investigated since 1965. These record major vegetation shifts at 28,000, 17,000‒14,000 and 9,000 years ago and a variable history of human disturbance from 14,000 years ago with extensive clearance by the mid-Holocene at some sites. While montane peatlands were important agricultural centres in the Holocene, the introduction of new dryland crops has resulted in the abandonment of some peatlands in the last few centuries. Despite several decades of research, detailed knowledge of the mountain peatlands is poor and this is an obstacle to scientific management.

  2. [Arbovirus circulation in the Republic of Guinea].

    Science.gov (United States)

    Butenko, A M

    1996-01-01

    In 1978-1991 the USSR-Guinea Virological and Microbiological Laboratory functioned in Kindia, the Republic of Guinea. Arbovirus activity in this country was studied by a number of virologists and other specialists. Their personal contribution and achievements in this collaboration are reflected in the present paper. About 74,000 mosquitoes, 100,000 Ixodidae ticks, 1,500 wild birds, 2,700 bats, 106 monkeys, and 308 other mammals, 927 blood samples collected from febrile patients were examined in 1978-1989, using inoculation of new-born white mice. As a result of this work 127 strains of the following arboviruses were isolated: Chikungunia (1 strain), Dengue 2 (4), Saboya (7), Wesselsbron (1), Bunyamwera (4), M'Poko (5), Rift Valley Fever (6), CHF-Congo (9), Dugbe (22), Bhanja (6), Forecariah (2), Jos (26), Abadina (15), Kindia (2), Ark 6956 (1), Fomede (2), Bluetongue (9), Mossuril (2), AnK 6009 (1), and Kolente (2). Dengue 2, Wesselsbron, Bunyamwera, M'Poko, Kindia, Mossuril viruses were isolated from mosquitoes. Ixodidae ticks were sources for isolation of Chikungunia, Saboya, CCHF, Dugbe, Bhanja, Forecaciah, Jos, Abadina, Kindia, Ark 6956, Fomede, Bluetongue, and Kolente viruses. Saboya, RVF, Fomede, Kolente, AnK 6909 were isolated from bats (Chiroptera); Saboya, Abadina, and Bluetongue viruses from birds. One strain of Dugbe virus was originated from the brain of Cercopithecus patas. Bunyamwera and Abadina viruses were isolated from the blood of two febrile patients. Serological identification of many strains was kindly conducted at the Pasteur Institute, Dakar (J. P.Digoutte) and some at the YARU, USA (R. Shope). Kindia and Ark 6956 (Reovirus, gr. Palyam), Fomede (gr. Chobar Gorge), Forecariah (Bunyavirus, gr. Bhanja), Kolente (Rhabdovirus) were identified as an original type of Lagos bat virus. The results of seroepidemiological surveys are also presented.

  3. Descripción del intento de suicidio como constructo de violencia en la población pediátrica femenina

    Directory of Open Access Journals (Sweden)

    Luis F. Guerrero-Martínez

    2016-09-01

    Full Text Available Objetivo: caracterizar clínicamente el intento de suicidio desde el enfoque del constructo de violencia en la población pediátrica femenina. Metodología: estudio descriptivo, retrospectivo y transversal, multivariado, desarrollado en el Hospital Infantil Los Ángeles de la ciudad de Pasto (Nariño, sobre la atención de pacientes femeninas entre 10 y 17 años, con diagnóstico de intento de suicidio, entre los años 2009 a 2014. Resultados: 97 pacientes (IC: 95%, Z=1,96, e: 10, p=50%. Mayor incidencia entre los 13 y 16 años (88%, predominio año 2011 (37% y en los meses de abril, mayo, julio y agosto. Municipios de Nariño con mayor número de casos: Pasto, La Unión y El Tambo (56%. Putumayo (21%. Intentos de suicidio previos (33%. Mecanismo mayormente utilizado: ingestión de tóxicos y medicamentos (92%. Como factores de riesgo emocional: las discusiones con familiares (32%, las relaciones afectivas fallidas (14% y la convivencia diferente a sus padres (17%, representan más del 88%. Subregistro en la determinación de violencia intrafamiliar, abuso sexual y consumo de sustancias psicoactivas (70%. Valoración por parte de psicología (98%, y por psiquiatría (65%, frente a una ideación suicida y seguimientos menores del 5%. Discusión: la caracterización clínica del intento de suicidio desde el constructo de violencia, teniendo en cuenta el análisis de los diferentes factores de riesgo, permitirán identificar el abordaje clínico de las pacientes, fuente del adecuado plan de tratamiento, seguimiento y pautas de prevención, como base correlativa y reproducible para el manejo multidisciplinario.

  4. Prevalence of fur mites (Chirodiscoides caviae) in pet guinea pigs (Cavia porcellus) in southern Italy.

    Science.gov (United States)

    d'Ovidio, Dario; Santoro, Domenico

    2014-04-01

    Chirodiscoides caviae is the most common fur mite affecting guinea pigs; infestation is generally asymptomatic. No studies have been published on the prevalence of such mites in guinea pigs in southern Italy. We sought to evaluate the prevalence and the clinical signs of C. caviae infestation in guinea pigs in southern Italy. Clinical records of guinea pigs evaluated from August 2012 to July 2013 were retrospectively searched. In this retrospective matched case-control study, records of guinea pigs with evidence of C. caviae infestation were selected. The prevalence of C. caviae infestation was evaluated and exposure variables were assessed among guinea pigs with and without infestation using stepwise conditional logistic regression. Guinea pigs seen during the same time period, but without a diagnosis of C. caviae, were included as control animals. The prevalence of C. caviae was 32% (42 of 131); 66.6% of affected guinea pigs (28 of 42) originated from pet shops, whereas 28% (14 of 42) were privately owned. Thirty-one guinea pigs (73.8%) were asymptomatic, whereas 11 (26.1%) showed clinical signs (pruritus, alopecia, erythema and scaling). The most frequently affected area was the lumbosacral region (38 of 42). Guinea pigs in pet shops were more likely to be affected by C. caviae than owned guinea pigs (odds ratio, 5.12; 95% confidence interval, 2.32-11.29; P guinea pigs in southern Italy. Chirodiscoides mites should be sought in guinea pigs, particularly in animals coming from pet shops. © 2014 ESVD and ACVD.

  5. Fracionamento dos carboidratos e cinética de degradação in vitro da fibra em detergente neutro da extrusa de bovinos a pasto

    Directory of Open Access Journals (Sweden)

    Vieira Ricardo Augusto Mendonça

    2000-01-01

    Full Text Available Os objetivos do presente trabalho foram a caracterização e a determinação das estimativas dos parâmetros relativos à cinética de degradação ruminal dos carboidratos, em amostras de extrusa de animais mantidos em pastagem natural da Zona da Mata (MG, nas estações chuvosa e seca do ano. Para obtenção de amostras representativas, foram usados quatro animais fistulados no esôfago durante dois períodos experimentais na estação chuvosa, e três destes animais por dois períodos experimentais na estação seca, do ano de 1996. Foi efetuado o fracionamento dos carboidratos de acordo com o sistema Cornell e determinadas as taxas de degradação da fibra em detergente neutro (FDN, a partir dos perfis de degradação in vitro deste componente. Foram determinadas as frações potencialmente degradável (B2 e indegradável (C da FDN, corrigida para proteína e cinzas, e as taxas de latência dinâmica, degradação e crescimento específico dos microrganismos, a partir da fração B2, além dos coeficientes de degradabilidade e do efeito de repleção ruminal da FDN. Observou-se que os carboidratos não-estruturais compreenderam pequena fração dos carboidratos totais nas amostras do pasto, em ambas as estações. Os carboidratos estruturais foram responsáveis pelo aumento no teor de carboidratos totais da extrusa, com a mudança da estação chuvosa para a estação seca; contudo, por causa do aumento da fração C e da menor taxa de crescimento específico dos microrganismos durante a estação seca, foi possível notar redução da degradabilidade e aumento do efeito de repleção ruminal da FDN da extrusa.

  6. Acúmulo de forragem em pastos de Brachiaria decumbens adubados com nitrogênio Biomass of the forage in Brachiaria decumbens pastures fertilized with nitrogen

    Directory of Open Access Journals (Sweden)

    Jailson Lara Fagundes

    2005-04-01

    Full Text Available A dinâmica de crescimento de plantas forrageiras tem sido foco de estudo nos últimos anos, visando aprimorar o conhecimento do processo de produção de forragem em pastagens. O objetivo deste trabalho foi avaliar o acúmulo de forragem em pastos de Brachiaria decumbens Stapf. adubados com nitrogênio e submetidos a uma mesma intensidade de pastejo. Os tratamentos consistiram de 75, 150, 225 e 300 kg ha-1 ano-1 de N, aplicados antes do início das avaliações experimentais, as quais foram realizadas durante as estações de verão, outono, inverno e primavera de 2002. O delineamento experimental utilizado foi o de blocos completos casualizados com duas repetições. O capim-braquiária apresentou incremento de produção de matéria seca proporcional às doses de nitrogênio. Observaram-se maiores valores de taxa de acúmulo de folha, colmo e forragem nas estações primavera-verão e valores menores no inverno. As variações nas condições climáticas com as estações do ano alteraram as taxas de acúmulo de folha e colmo, senescência e de produção de forragem em Brachiaria decumbens.In the last years, the growth dynamics of forage plants has been focused, in order to improve the knowledge of forage yield and distribution process in pasture. The objective of this work was to evaluate the effect of nitrogen fertilization on accumulation of the forage in Brachiaria decumbens Stapf. pastures submitted to the same stocking rate. Treatments consisted of 75, 150, 225 and 300 kg ha-1 yr-1 N applied before the beginning of experimental evaluations, which were performed during summer, autumn, winter and spring of 2002. A randomized block experimental design was used with two replicates. Brachiaria grass showed an increase in the dry matter yield proportionally to nitrogen doses, with higher rates for daily accumulation of leaves, stems and forage yield in summer-spring seasons, while lower rates were shown in the winter. The variation of

  7. Variación de la concentración del furadán en el perfil de un andisol

    OpenAIRE

    Góez G., Gloria Patricia; Zapata H., Raúl; González García, Jairo

    1999-01-01

    Con el objetivo de determinar el movimiento del Furadán en el suelo, se muestrearon las fases sólidas y líquidas de dos Andisoles ubicados bajo pasto y bajo bosque. El muestreo se hizo instalando un lisímetro en cada horizonte, luego se aplicaron 3 mL de Furadán disueltos en 6 litros de agua, equivalentes a una concentración de 181.1 ?g/ml, en la superficie de los dos Andisoles. El monitoreo se realizó durante seis meses. Para extraer el Furadán de las fases líquida y sólida del suelo se usar...

  8. 9 CFR 3.36 - Primary enclosures used to transport live guinea pigs and hamsters.

    Science.gov (United States)

    2010-01-01

    ... live guinea pigs and hamsters. 3.36 Section 3.36 Animals and Animal Products ANIMAL AND PLANT HEALTH..., Care, Treatment, and Transportation of Guinea Pigs and Hamsters Transportation Standards § 3.36 Primary enclosures used to transport live guinea pigs and hamsters. No person subject to the Animal Welfare...

  9. Use of a Guinea pig-specific transcriptome array for evaluation of protective immunity against genital chlamydial infection following intranasal vaccination in Guinea pigs.

    Science.gov (United States)

    Wali, Shradha; Gupta, Rishein; Veselenak, Ronald L; Li, Yansong; Yu, Jieh-Juen; Murthy, Ashlesh K; Cap, Andrew P; Guentzel, M Neal; Chambers, James P; Zhong, Guangming; Rank, Roger G; Pyles, Richard B; Arulanandam, Bernard P

    2014-01-01

    Guinea pigs have been used as a second animal model to validate putative anti-chlamydial vaccine candidates tested in mice. However, the lack of guinea pig-specific reagents has limited the utility of this animal model in Chlamydia sp. vaccine studies. Using a novel guinea pig-specific transcriptome array, we determined correlates of protection in guinea pigs vaccinated with Chlamydia caviae (C. caviae) via the intranasal route, previously reported by us and others to provide robust antigen specific immunity against subsequent intravaginal challenge. C. caviae vaccinated guinea pigs resolved genital infection by day 3 post challenge. In contrast, mock vaccinated animals continued to shed viable Chlamydia up to day 18 post challenge. Importantly, at day 80 post challenge, vaccinated guinea pigs experienced significantly reduced genital pathology - a sequelae of genital chlamydial infections, in comparison to mock vaccinated guinea pigs. Sera from vaccinated guinea pigs displayed antigen specific IgG responses and increased IgG1 and IgG2 titers capable of neutralizing GPIC in vitro. Th1-cellular/inflammatory immune genes and Th2-humoral associated genes were also found to be elevated in vaccinated guinea pigs at day 3 post-challenge and correlated with early clearance of the bacterium. Overall, this study provides the first evidence of guinea pig-specific genes involved in anti-chlamydial vaccination and illustrates the enhancement of the utility of this animal model in chlamydial pathogenesis.

  10. Hypervitaminosis D in Guinea Pigs with α-Mannosidosis

    Science.gov (United States)

    Jensen, JanLee A; Brice, Angela K; Bagel, Jessica H; Mexas, Angela M; Yoon, Sea Young; Wolfe, John H

    2013-01-01

    A colony of guinea pigs (n = 9) with α-mannosidosis was fed a pelleted commercial laboratory guinea pig diet. Over 2 mo, all 9 guinea pigs unexpectedly showed anorexia and weight loss (11.7% to 30.0% of baseline weight), and 3 animals demonstrated transient polyuria and polydipsia. Blood chemistry panels in these 3 guinea pigs revealed high-normal total calcium, high-normal phosphate, and high ALP. Urine specific gravity was dilute (1.003, 1.009, 1.013) in the 3 animals tested. Postmortem examination of 7 animals that were euthanized after failing to respond to supportive care revealed renal interstitial fibrosis with tubular mineralization, soft tissue mineralization in multiple organs, hepatic lipidosis, and pneumonia. Analysis of the pelleted diet revealed that it had been formulated with a vitamin D3 content of more than 150 times the normal concentration. Ionized calcium and 25-hydroxyvitamin D values were both high in serum saved from 2 euthanized animals, confirming the diagnosis of hypervitaminosis D. This report discusses the clinical signs, blood chemistry results, and gross and histologic findings of hypervitaminosis D in a colony of guinea pigs. When unexpected signs occur colony-wide, dietary differentials should be investigated at an early time point. PMID:23582422

  11. Evaluation of Ebola Virus Countermeasures in Guinea Pigs.

    Science.gov (United States)

    Marzi, Andrea

    2017-01-01

    Ebola virus (EBOV) pathology in humans remains incompletely understood; therefore, a number of rodent and nonhuman primate (NHP) models have been established to study the disease caused by this virus. While the macaque model most accurately recapitulates human disease, rodent models, which display only certain aspects of human disease but are more cost-effective, are widely used for initial screens during EBOV countermeasure development. In particular, mice and guinea pigs were among the first species used for the efficacy testing of EBOV vaccines and therapeutics. While mice have low predictive value, guinea pigs have proven to be a more reliable predictor for the evaluation of countermeasures in NHPs. In addition, guinea pigs are larger in size compared to mice, allowing for more frequent collection of blood samples at larger volumes. However, guinea pigs have the disadvantage that there is only a limited pool of immunological tools available to characterize host responses to vaccination, treatment and infection. In this chapter, the efficacy testing of an EBOV vaccine and a therapeutic in the guinea pig model are described.

  12. Non-terminal blood sampling techniques in guinea pigs.

    Science.gov (United States)

    Birck, Malene M; Tveden-Nyborg, Pernille; Lindblad, Maiken M; Lykkesfeldt, Jens

    2014-10-11

    Guinea pigs possess several biological similarities to humans and are validated experimental animal models(1-3). However, the use of guinea pigs currently represents a relatively narrow area of research and descriptive data on specific methodology is correspondingly scarce. The anatomical features of guinea pigs are slightly different from other rodent models, hence modulation of sampling techniques to accommodate for species-specific differences, e.g., compared to mice and rats, are necessary to obtain sufficient and high quality samples. As both long and short term in vivo studies often require repeated blood sampling the choice of technique should be well considered in order to reduce stress and discomfort in the animals but also to ensure survival as well as compliance with requirements of sample size and accessibility. Venous blood samples can be obtained at a number of sites in guinea pigs e.g., the saphenous and jugular veins, each technique containing both advantages and disadvantages(4,5). Here, we present four different blood sampling techniques for either conscious or anaesthetized guinea pigs. The procedures are all non-terminal procedures provided that sample volumes and number of samples do not exceed guidelines for blood collection in laboratory animals(6). All the described methods have been thoroughly tested and applied for repeated in vivo blood sampling in studies within our research facility.

  13. A guinea pig model of Zika virus infection.

    Science.gov (United States)

    Kumar, Mukesh; Krause, Keeton K; Azouz, Francine; Nakano, Eileen; Nerurkar, Vivek R

    2017-04-11

    Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemic of Zika virus (ZIKV). Here we report that immunocompetent guinea pigs are susceptible to infection by a contemporary American strain of ZIKV. Dunkin-Hartley guinea pigs were inoculated with 10 6 plaque-forming units of ZIKV via subcutaneous route and clinical signs were observed. Viremia, viral load in the tissues, anti-ZIKV neutralizing antibody titer, and protein levels of multiple cytokine and chemokines were analyzed using qRT-PCR, plaque assay, plaque reduction neutralization test (PRNT) and multiplex immunoassay. Upon subcutaneous inoculation with PRVABC59 strain of ZIKV, guinea pigs demonstrated clinical signs of infection characterized by fever, lethargy, hunched back, ruffled fur, and decrease in mobility. ZIKV was detected in the whole blood and serum using qRT-PCR and plaque assay. Anti-ZIKV neutralizing antibody was detected in the infected animals using PRNT. ZIKV infection resulted in a dramatic increase in protein levels of multiple cytokines, chemokines and growth factors in the serum. ZIKV replication was observed in spleen and brain, with the highest viral load in the brain. This data demonstrate that after subcutaneous inoculation, the contemporary ZIKV strain is neurotropic in guinea pigs. The guinea pig model described here recapitulates various clinical features and viral kinetics observed in ZIKV-infected patients, and therefore may serve as a model to study ZIKV pathogenesis, including pregnancy outcomes and for evaluation of vaccines and therapeutics.

  14. Inner ear test battery in guinea pig models - a review.

    Science.gov (United States)

    Young, Yi-Ho

    2018-06-01

    This study reviewed the development of the inner ear test battery comprising auditory brainstem response (ABR), and caloric, ocular vestibular-evoked myogenic potential (oVEMP), and cervical vestibular-evoked myogenic potential (cVEMP) tests in guinea pig models at our laboratory over the last 20 years. Detailed description of the methodology for testing the small animals is also included. Inner ear disorders, i.e. ototoxicity, noise exposure, or perilymph fistula were established in guinea pig models first. One to four weeks after operation, each animal underwent ABR, oVEMP, cVEMP, and caloric tests. Then, animals were sacrificed for morphological study in the temporal bones. Inner ear endorgans can be comprehensively evaluated in guinea pig models via an inner ear test battery, which provides thorough information on the cochlea, saccule, utricle, and semicircular canal function of guinea pigs. Coupled with morphological study in the temporal bones of the animals may help elucidate the mechanism of inner ear disorders in humans. The inner ear test battery in guinea pig models may encourage young researchers to perform basic study in animals and stimulate the progress of experimental otology which is in evolution.

  15. Sex Genotyping of Archival Fixed and Immunolabeled Guinea Pig Cochleas.

    Science.gov (United States)

    Depreux, Frédéric F; Czech, Lyubov; Whitlon, Donna S

    2018-03-26

    For decades, outbred guinea pigs (GP) have been used as research models. Various past research studies using guinea pigs used measures that, unknown at the time, may be sex-dependent, but from which today, archival tissues may be all that remain. We aimed to provide a protocol for sex-typing archival guinea pig tissue, whereby past experiments could be re-evaluated for sex effects. No PCR sex-genotyping protocols existed for GP. We found that published sequence of the GP Sry gene differed from that in two separate GP stocks. We used sequences from other species to deduce PCR primers for Sry. After developing a genomic DNA extraction for archival, fixed, decalcified, immunolabeled, guinea pig cochlear half-turns, we used a multiplex assay (Y-specific Sry; X-specific Dystrophin) to assign sex to tissue as old as 3 years. This procedure should allow reevaluation of prior guinea pig studies in various research areas for the effects of sex on experimental outcomes.

  16. Radiation induced micrencephaly in guinea pigs

    International Nuclear Information System (INIS)

    Wagner, L.K.; Johnston, D.A.; Felleman, D.J.

    1991-01-01

    A brain weight deficit of about 70 mg was induced at doses of approximately 75-mGy and a deficit of 60 mg was induced at 100 mGy. This confirms the effects projected and observed by Wanner and Edwards. Although the data do not demonstrate a clear dose-response relationship between the 75-mGy and 100-mGy groups, the data are statistically consistent with a dose-response effect because of the overlapping confidence intervals. The lack of a statistically significant observation is most likely related to the small difference in doses and the limited numbers of animals examined. There are several factors that can influence the brain weight of guinea pig pups, such as caging and housing conditions, the sex of the animal, and litter size. These should be taken into account for accurate analysis. Dam weight did not appear to have a significant effect. The confirmation of a micrencephalic effect induced x rays at doses of 75-mGy during this late embryonic stage of development is consistent with the findings of small head size induced in those exposed prior to the eight week of conception at Hiroshima. This implies a mechanism for micrencephaly different from those previously suggested and lends credence to a causal relation between radiation and small head size in humans at low doses as reported by Miller and Mulvihill. 16 refs., 13 tabs

  17. Radiation protection in hospitals of Equatorial Guinea

    International Nuclear Information System (INIS)

    Rabat Macambo, P.

    2001-01-01

    With a population of four hundred thousand (400.000) inhabitants and distributed in a territory of 28 thousand (28.000) km 2 , the use of ionizing radiations for medical practice in Equatorial Guinea is few and decreasing. It is used for diagnostic practices in the main hospitals of the country, where the work burden is not over 20 patients per day. The political, social and economical embryonic development of the country until recently had a negative influence on indicators and health organisations, so that even now the country does not have any radiological protection law, this shortness, in addition with the old architectural structure that x ray tools is lodging, as well as dosimetrical lack of employed staff, put this staff under risk of electromagnetic energy. This is to show the present survey of medical activities with ionizing radiation and to request technical support for implementing suitably the basic standards of radiation protection which will help us as basis for the elaboration outline law, on radiological protection in accordance with the new guidelines of the International Atomic Energy Agency. (author)

  18. Influencia de la intensidad de uso sobre algunas propiedades físicas en un suelo del Valle del Cauca, Colombia

    Directory of Open Access Journals (Sweden)

    Alveiro Salamanca Jiménez

    2015-03-01

    Full Text Available La intensidad de uso y manejo del suelo generalmente altera el comportamiento de sus propiedades físicas y acelera su degradación. Con el fin de medir el impacto de dicha intensidad, se llevó a cabo un muestreo en la estación experimental del CIAT-Palmira, Valle del Cauca, Colombia. Se escogieron seis lotes con diferentes historiales, desde uso semestral muy intensivo hasta uso reducido con descanso por más de 10 años, y se tomaron muestras de suelo a inco profundidades del perfil para evaluar las siguientes propiedades:conductividad hidráulica, permeabilidad al aire, porosidad total, porosidad drenable, compactabilidad, porosidad residual, materia orgánica y resistenciamecánica a la penetración. Se encontró que el uso de maquinaria y las labores de fangueo mejoraron las propiedades físicas sólo en los 15 cm superficiales, pero han causado graves problemas de compactación en las profundidadesinferiores. El pisoteo del ganado también ha compactado el perfil, limitando las condiciones físicas para ser explorado por las raíces de los pastos.Los períodos de descanso han favorecido la estabilidad estructural del suelo y con ello la calidad del mismo. Se recomienda implementar prácticastendientes a reducir los riesgos por compactación, que ayuden a frenar la degradación y a recuperar la capacidad productiva de estos suelos.

  19. Analyzing Protein Changes in Guinea Pig Tissue Lysates Using Non-guinea Pig Specific Antibodies: Procedures for Western Blotting and Examples Using 16 Individual Antibodies for Common CNS Proteins

    Science.gov (United States)

    2006-05-01

    guinea pig model does present a significant problem...trying to correlate behavioral and protein changes due to the absence of guinea pig -specific antibodies. We...have developed a procedure to determine the specificity of commercially available, non- guinea pig -specific antibodies in guinea pig lysates.

  20. Utilização de Três Fontes de Nitrogênio Associadas à Palma Forrageira (Opuntia ficus-indica, Mill. Cv. Gigante na Suplementação de Vacas Leiteiras Mantidas em Pasto Diferido

    Directory of Open Access Journals (Sweden)

    Albuquerque Samuel Salgado Cavalcanti de

    2002-01-01

    Full Text Available O fornecimento da palma forrageira como único alimento não atende as necessidades nutricionais do rebanho, notadamente em proteína e fibra. Nesta pesquisa avaliaram-se fontes de suplementação protéica sobre o desempenho de vacas leiteiras alimentadas com palma e pasto diferido, durante a estação seca, bem como a composição botânica da pastagem. Testaram-se três tratamentos: T1 = pasto diferido (PD + palma + cama de aviário; T2 = PD + palma + farelo de soja e T3 = PD + palma + uréia/sulfato de amônio, em um delineamento de blocos casualizados. Utilizaram-se 12 vacas mestiças 5/8 Holando-Zebu, com produção média de leite de 5,6 kg/dia/vaca e peso vivo (PV médio de 452 kg. O experimento teve duração de 121 dias, sendo 21 dias de período de adaptação. Os resultados para produção de leite in natura (3,38, 5,33 e 3,38 kg/vaca/dia, produção de leite corrigido para 4% de gordura (3,15, 4,98 e 3,11 kg/vaca/dia, variação de peso vivo (-351, 77 e -401 g/vaca/dia e consumo in natura de palma (26,3, 37,8 e 26,3 kg/vaca/dia, para T1, T2 e T3, respectivamente, demonstraram a superioridade do farelo de soja como fonte protéica, em relação aos demais tratamentos. No pasto diferido foram identificados 18 famílias, 32 gêneros e 36 espécies. O capim-milhã (Brachiaria plantaginea (Link Hitch foi o componente de maior participação na composição botânica da pastagem (28,72%.

  1. Logistics of Guinea worm disease eradication in South Sudan.

    Science.gov (United States)

    Jones, Alexander H; Becknell, Steven; Withers, P Craig; Ruiz-Tiben, Ernesto; Hopkins, Donald R; Stobbelaar, David; Makoy, Samuel Yibi

    2014-03-01

    From 2006 to 2012, the South Sudan Guinea Worm Eradication Program reduced new Guinea worm disease (dracunculiasis) cases by over 90%, despite substantial programmatic challenges. Program logistics have played a key role in program achievements to date. The program uses disease surveillance and program performance data and integrated technical-logistical staffing to maintain flexible and effective logistical support for active community-based surveillance and intervention delivery in thousands of remote communities. Lessons learned from logistical design and management can resonate across similar complex surveillance and public health intervention delivery programs, such as mass drug administration for the control of neglected tropical diseases and other disease eradication programs. Logistical challenges in various public health scenarios and the pivotal contribution of logistics to Guinea worm case reductions in South Sudan underscore the need for additional inquiry into the role of logistics in public health programming in low-income countries.

  2. Helminths of the guinea pig, Cavia porcellus (Linnaeus, in Brazil

    Directory of Open Access Journals (Sweden)

    Roberto Magalhães Pinto

    2002-07-01

    Full Text Available Worm burdens were evaluated and compared in two groups of the guinea pig, Cavia poreellus (Linnaeus, 1758: animals of the first group were conventionally maintained in an institutional animal house and those of the second group were openly kept in pet shops in Brazil. Animals from both sources were infected only with the nematode Paraspidodera uncinata (Rudolphi, 1819 Travassos, 1914 (10% of prevalence in guinea pigs from lhe institutional facility and 40% in those animals from the pet shop. Other helminth samples recovered from Brazilian guinea pigs during 52 years and that are deposited in the Helminthological Collection of the Oswaldo Cruz Institute (CHIOC were also analyzed. Paraspidodera uncinata and the cestode Monoecocestus parcitesticulatus Rêgo, 1960 were identified in these samples.

  3. Developing guinea pig brain as a model for cortical folding.

    Science.gov (United States)

    Hatakeyama, Jun; Sato, Haruka; Shimamura, Kenji

    2017-05-01

    The cerebral cortex in mammals, the neocortex specifically, is highly diverse among species with respect to its size and morphology, likely reflecting the immense adaptiveness of this lineage. In particular, the pattern and number of convoluted ridges and fissures, called gyri and sulci, respectively, on the surface of the cortex are variable among species and even individuals. However, little is known about the mechanism of cortical folding, although there have been several hypotheses proposed. Recent studies on embryonic neurogenesis revealed the differences in cortical progenitors as a critical factor of the process of gyrification. Here, we investigated the gyrification processes using developing guinea pig brains that form a simple but fundamental pattern of gyri. In addition, we established an electroporation-mediated gene transfer method for guinea pig embryos. We introduce the guinea pig brain as a useful model system to understand the mechanisms and basic principle of cortical folding. © 2017 Japanese Society of Developmental Biologists.

  4. Radiation-induced bone tumours in the guinea-pig

    International Nuclear Information System (INIS)

    Knowles, J.F.

    1981-01-01

    A remarkably high proportion of guinea-pigs given localized irradiations of 20 Gy x-rays developed bone tumours, 46% of all irradiated with 20 Gy and 86% of those that survived at least a year. Untreated controls were not included in the present experiment, but the authors refer to an earlier experiment using guinea-pigs from the same colony where no bone tumour occurred in 69 unirradiated animals followed for their natural life span i.e. up to 87 months. It is concluded that the author's strain of guinea-pig (details given in a previous paper, Int. J. Radiol. Biol., 40, 265) is particularly prone to radiation-induced bone tumours. Their possible value for investigating processes associated with radiation induction of bone tumours is further enhanced by their relatively large size and long life span (up to 7 years). (U.K.)

  5. Jejuno-jejunal intussusception in a guinea pig (Cavia porcellus

    Directory of Open Access Journals (Sweden)

    Tara J. Fetzer

    2017-09-01

    Full Text Available An approximately four-year-old male castrated guinea pig (Cavia porcellus was presented for painful defecation with a 24-hour history of hyporexia and intermittent episodes of rolling behavior. Upon presentation the patient was quiet, alert, and responsive, and mildly hypothermic. Abdominal palpation revealed an approximately 2-cm long oblong mass within the caudal abdomen. Abdominal radiographs revealed gastric dilation without volvulus and a peritoneal mass effect. The patient was euthanized following gastric reflux of brown malodorous fluid from his nares and oral cavity. A necropsy was performed and revealed a jejuno-jejunal intussusception causing mechanical gastrointestinal ileus, and gastric dilatation without volvulus. While non-obstructive gastrointestinal stasis is common and obstructive ileus is uncommon in guinea pigs, this report shows that intestinal intussusception is a differential in guinea pigs with ileus and gastric dilatation.

  6. Bacterias celulolíticas aisladas del intestino de termitas (Nasutitermes nigriceps con características probióticas y potencial en la degradación del pasto

    Directory of Open Access Journals (Sweden)

    Cecilia Lara Mantilla

    2013-01-01

    Abstract: The objective of this research was to isolate cellulolytic bacteria from the gut of termites (Nasutitermes nigriceps to determine their probiotic properties in vitro and its potential in degrading  grass. Termites were treated with antibacterial detergent, then their intestine was separated and macerated in sterile peptone water. Dilutions of this mixture were inoculated in Petri dishes using  Luria Bertani (LB method and carboxymethylcellulose (CMC 2%, incubated at 37 ° C for 24 hours, and then revealed with 1% Congo Red. Bacteria with higher degradation halos were subjected to Gram staining and probiotic temperature tests, pH, salinity and the presence of bile salts, as well as antagonism and degradation of grass tests. The results revealed the presence of 9 Gram (-  cellulolytic bacillifrom which the bacilli BTN7 and BTN8, showed the best degradation halosof 12 and 14 mm in diameter respectively, growing suitably in the different probiotic tests with densities between 106 and 108 CFU / ml, the degradation percentages of dry matter was 39.73% and 36.10% within 48 and 72 hours respectively. Biochemical tests API E showed that (bioMérieux SA bacilli BTN8 and BTN7 belong to the genus Enterobacter sp. The above mentioned results open the possibility of using these organisms as additives for ruminants feeding, in order to contribute to a better use of pasture, and other lignocellulosic vegetal substrates. Key words:Probiotics, Maralfalfa (Pennisetum sp., ruminal microbes, lignocellulose, Enterobacter.

  7. Guinea pig ID-like families of SINEs.

    Science.gov (United States)

    Kass, David H; Schaetz, Brian A; Beitler, Lindsey; Bonney, Kevin M; Jamison, Nicole; Wiesner, Cathy

    2009-05-01

    Previous studies have indicated a paucity of SINEs within the genomes of the guinea pig and nutria, representatives of the Hystricognathi suborder of rodents. More recent work has shown that the guinea pig genome contains a large number of B1 elements, expanding to various levels among different rodents. In this work we utilized A-B PCR and screened GenBank with sequences from isolated clones to identify potentially uncharacterized SINEs within the guinea pig genome, and identified numerous sequences with a high degree of similarity (>92%) specific to the guinea pig. The presence of A-tails and flanking direct repeats associated with these sequences supported the identification of a full-length SINE, with a consensus sequence notably distinct from other rodent SINEs. Although most similar to the ID SINE, it clearly was not derived from the known ID master gene (BC1), hence we refer to this element as guinea pig ID-like (GPIDL). Using the consensus to screen the guinea pig genomic database (Assembly CavPor2) with Ensembl BlastView, we estimated at least 100,000 copies, which contrasts markedly to just over 100 copies of ID elements. Additionally we provided evidence of recent integrations of GPIDL as two of seven analyzed conserved GPIDL-containing loci demonstrated presence/absence variants in Cavia porcellus and C. aperea. Using intra-IDL PCR and sequence analyses we also provide evidence that GPIDL is derived from a hystricognath-specific SINE family. These results demonstrate that this SINE family continues to contribute to the dynamics of genomes of hystricognath rodents.

  8. Guinea Pig Oxygen-Sensing and Carotid Body Functional Properties

    Science.gov (United States)

    Gonzalez-Obeso, Elvira; Docio, Inmaculada; Olea, Elena; Cogolludo, Angel; Obeso, Ana; Rocher, Asuncion; Gomez-Niño, Angela

    2017-01-01

    Mammals have developed different mechanisms to maintain oxygen supply to cells in response to hypoxia. One of those mechanisms, the carotid body (CB) chemoreceptors, is able to detect physiological hypoxia and generate homeostatic reflex responses, mainly ventilatory and cardiovascular. It has been reported that guinea pigs, originally from the Andes, have a reduced ventilatory response to hypoxia compared to other mammals, implying that CB are not completely functional, which has been related to genetically/epigenetically determined poor hypoxia-driven CB reflex. This study was performed to check the guinea pig CB response to hypoxia compared to the well-known rat hypoxic response. These experiments have explored ventilatory parameters breathing different gases mixtures, cardiovascular responses to acute hypoxia, in vitro CB response to hypoxia and other stimuli and isolated guinea pig chemoreceptor cells properties. Our findings show that guinea pigs are hypotensive and have lower arterial pO2 than rats, probably related to a low sympathetic tone and high hemoglobin affinity. Those characteristics could represent a higher tolerance to hypoxic environment than other rodents. We also find that although CB are hypo-functional not showing chronic hypoxia sensitization, a small percentage of isolated carotid body chemoreceptor cells contain tyrosine hydroxylase enzyme and voltage-dependent K+ currents and therefore can be depolarized. However hypoxia does not modify intracellular Ca2+ levels or catecholamine secretion. Guinea pigs are able to hyperventilate only in response to intense acute hypoxic stimulus, but hypercapnic response is similar to rats. Whether other brain areas are also activated by hypoxia in guinea pigs remains to be studied. PMID:28533756

  9. Guinea Pig Oxygen-Sensing and Carotid Body Functional Properties.

    Science.gov (United States)

    Gonzalez-Obeso, Elvira; Docio, Inmaculada; Olea, Elena; Cogolludo, Angel; Obeso, Ana; Rocher, Asuncion; Gomez-Niño, Angela

    2017-01-01

    Mammals have developed different mechanisms to maintain oxygen supply to cells in response to hypoxia. One of those mechanisms, the carotid body (CB) chemoreceptors, is able to detect physiological hypoxia and generate homeostatic reflex responses, mainly ventilatory and cardiovascular. It has been reported that guinea pigs, originally from the Andes, have a reduced ventilatory response to hypoxia compared to other mammals, implying that CB are not completely functional, which has been related to genetically/epigenetically determined poor hypoxia-driven CB reflex. This study was performed to check the guinea pig CB response to hypoxia compared to the well-known rat hypoxic response. These experiments have explored ventilatory parameters breathing different gases mixtures, cardiovascular responses to acute hypoxia, in vitro CB response to hypoxia and other stimuli and isolated guinea pig chemoreceptor cells properties. Our findings show that guinea pigs are hypotensive and have lower arterial pO 2 than rats, probably related to a low sympathetic tone and high hemoglobin affinity. Those characteristics could represent a higher tolerance to hypoxic environment than other rodents. We also find that although CB are hypo-functional not showing chronic hypoxia sensitization, a small percentage of isolated carotid body chemoreceptor cells contain tyrosine hydroxylase enzyme and voltage-dependent K + currents and therefore can be depolarized. However hypoxia does not modify intracellular Ca 2+ levels or catecholamine secretion. Guinea pigs are able to hyperventilate only in response to intense acute hypoxic stimulus, but hypercapnic response is similar to rats. Whether other brain areas are also activated by hypoxia in guinea pigs remains to be studied.

  10. Immunoglobulin Genomics in the Guinea Pig (Cavia porcellus)

    Science.gov (United States)

    Guo, Yongchen; Bao, Yonghua; Meng, Qingwen; Hu, Xiaoxiang; Meng, Qingyong; Ren, Liming; Li, Ning; Zhao, Yaofeng

    2012-01-01

    In science, the guinea pig is known as one of the gold standards for modeling human disease. It is especially important as a molecular and cellular biology model for studying the human immune system, as its immunological genes are more similar to human genes than are those of mice. The utility of the guinea pig as a model organism can be further enhanced by further characterization of the genes encoding components of the immune system. Here, we report the genomic organization of the guinea pig immunoglobulin (Ig) heavy and light chain genes. The guinea pig IgH locus is located in genomic scaffolds 54 and 75, and spans approximately 6,480 kb. 507 VH segments (94 potentially functional genes and 413 pseudogenes), 41 DH segments, six JH segments, four constant region genes (μ, γ, ε, and α), and one reverse δ remnant fragment were identified within the two scaffolds. Many VH pseudogenes were found within the guinea pig, and likely constituted a potential donor pool for gene conversion during evolution. The Igκ locus mapped to a 4,029 kb region of scaffold 37 and 24 is composed of 349 Vκ (111 potentially functional genes and 238 pseudogenes), three Jκ and one Cκ genes. The Igλ locus spans 1,642 kb in scaffold 4 and consists of 142 Vλ (58 potentially functional genes and 84 pseudogenes) and 11 Jλ -Cλ clusters. Phylogenetic analysis suggested the guinea pig’s large germline VH gene segments appear to form limited gene families. Therefore, this species may generate antibody diversity via a gene conversion-like mechanism associated with its pseudogene reserves. PMID:22761756

  11. ESTRUCTURA DE LAS PRADERAS DE THALASSIA TESTUDINUM EN LA ISLA DE PROVIDENCIA, CARIBE COLOMBIANO, DESPUÉS DEL PASO DEL HURACÁN BETA

    Directory of Open Access Journals (Sweden)

    Sierra O

    2012-06-01

    Full Text Available El 29 de octubre de 2005 el huracán Beta, de categoría 1 pasó a 15 km norte de la isla de Providencia, Caribe colombiano. Con el propósito de determinar eventuales cambios estructurales de las praderas de fanerógamas marinas, se monitorearon cuatro praderas de Thalassia testudinum Banks ex König alrededor de la isla, uno y dos años después del paso del huracán. Se seleccionaron dos sitios no afectados y dos afectados por el paso del huracán. Se observó que las praderas de pastos marinos en la isla de Providencia parecen sanas y sin signos de alteraciones significativas ocasionadas por el huracán Beta. La única posible alteración de tipo estructural, fue devolver a un estado sucesional más temprano a este ecosistema en las zonas afectadas, alteración que actualmente parece haberse superado.

  12. Molecular characterization of Cryptosporidium isolates from humans in Equatorial Guinea.

    Science.gov (United States)

    Blanco, María Alejandra; Iborra, Asunción; Vargas, Antonio; Nsie, Eugenia; Mbá, Luciano; Fuentes, Isabel

    2009-12-01

    The aim of the study was to perform a molecular characterization of clinical isolates of Cryptosporidium species from Equatorial Guinea. Standard laboratory methods were used to identify 35 cryptosporidiosis cases among 185 patients. PCR-RFLP successfully identified 34 Cryptosporidium species from these 35 cases, comprising C. parvum (52.9%), C. hominis (44.1%) and C. meleagridis (2.9%); over 90% of the species were isolated from HIV-positive patients. This is the first report of the molecular characterization of Cryptosporidium species isolated from humans in Equatorial Guinea and shows that zoonotic and anthroponotic transmission is present in this country.

  13. Good Order at Sea in the Gulf of Guinea

    DEFF Research Database (Denmark)

    Nordby, Johannes Riber; Lindskov Jacobsen, Katja

    In this chapter, we first outline the maritime security situation in the Gulf of Guinea region at present. It is examined in the context of Good Order at Sea. Second, we describe the most recent maritime security initiative launched during the Yaoundé Summit in 2013. During the summit, West...... and Central African states decided to establish six maritime zones along their coastline in order to improve the maritime security in the Gulf of Guinea. A process heavily supported by UN’s International Maritime Organisation (IMO). Finally, the chapter suggests that maritime security issues in the Gulf...

  14. Macromineral requirements by beef cattle under pasture supplementation Exigências de macrominerais de bovinos de corte sob suplementação a pasto

    Directory of Open Access Journals (Sweden)

    Maykel Franklin Lima Sales

    2011-02-01

    /animal/dia. A meia-carcaça direita de oito animais, dois por grupo, foi dissecada em músculo, gordura e ossos e todos os constituintes do corpo foram pesados para determinação do peso de corpo vazio e da composição corporal. As exigências líquidas de cálcio e fósforo para um animal de 400 kg de peso corporal foram, respectivamente, 11,13 g e 5,40 g. Para estimar as exigências dietéticas de mantença e, posteriormente, somá-las às exigências dietéticas para ganho, no intuito de se obter as exigências dietéticas totais, foram adotadas as perdas endógenas e a biodisponibilidade presentes na literatura para cada macroelemento mineral. As exigências dietéticas totais de cálcio, fósforo, magnesium, sódio e potássio para um animal de 400 kg de PC com ganho diário de 1,0 kg foram, respectivamente, 34,59; 17,36; 7,82; 5,19 e 41,11 g/dia. As exigências em macrominerais de bovinos de corte sob suplementação a pasto são semelhantes às recomendadas pelo BR-Corte para bovinos de corte em confinamento.

  15. Desempenho de novilhos nelore suplementados em pasto durante época das águas Performance of crossbreed nelore bullock grazing supplemented during the raining seazon

    Directory of Open Access Journals (Sweden)

    Wilker Dias Lima

    2004-02-01

    Full Text Available A procura por alimentos e derivados que não sejam utilizados na dieta humana ou na alimentação de animais monogástricos, juntamente com formas de suplementação, são aspectos importantes na busca de fontes alternativas para alimentação de ruminantes e redução no tempo de abate. Com este trabalho teve-se como objetivo avaliar o efeito de níveis crescentes de concentrado na engorda de novilhos nelore suplementados em pasto durante o período das águas. O experimento foi realizado na Fazenda Vitorinha, de propriedade da Fundação de Apoio ao Ensino, Pesquisa e Extensão/FAEPE, ligada à Universidade Federal de Lavras/UFLA, em Lavras, região sul do Estado de Minas Gerais, entre os meses de janeiro a maio de 2001. Foram utilizados 24 animais mestiços nelores, machos, inteiros, com idade média de 17 meses e peso vivo médio de 308,0 kg. Durante o período experimental, os animais foram alojados em uma pastagem de Brachiaria decumbens, com área de 11,2 ha, com disponibilidade média de 5400 kg MS/ha no início do período. Os tratamentos constituíram-se de níveis crescentes de concentrado, calculados como percentual do peso vivo: T1- 0%; T2- 0,15%; T3- 0,30%; T4- 0,45%. O experimento foi delineado em blocos casualizados, com peso vivo inicial como fator de blocagem, sendo 6 blocos e 4 tratamentos, totalizando 24 parcelas experimentais. Para análise dos dados, utilizou-se o software estatístico SISVAR (Sistema de Análise de Variância de Dados Balanceados. Não houve diferença significativa para ganho de peso diário (PThe demand for foods and its derivates that would not be used in the human diet or in the monogastric animals diet in addition with supplement forms are important aspects in the search for alternative sources for ruminant nutrition and reduction in the slaughtered time. This work had as objective to evaluate growing levels of concentrate in the fattening of nelore bullock gazing supplemented during the raining

  16. Produtividade e valor nutritivo de pastos consorciados com diferentes espécies de leguminosas Productivity and nutritive value of mixed pastures with different legume species

    Directory of Open Access Journals (Sweden)

    Clair Jorge Olivo

    2012-11-01

    Full Text Available O objetivo desta pesquisa foi avaliar a produtividade e o valor nutritivo de dois sistemas forrageiros (SF constituídos por capim elefante (CE, azevém (AZ, trevo branco (TB e espécies de crescimento espontâneo (ECE, como SF1; e CE + AZ + amendoim forrageiro (AF + ECE, como SF2. O CE foi estabelecido em linhas espaçadas a cada quatro metros. Entre elas, foi estabelecido azevém, durante o período hibernal, permitindo-se o desenvolvimento de ECE no período estival. Para avaliação, foram utilizadas vacas da raça Holandesa e o método de pastejo foi o rotacionado. Avaliaram-se a massa de forragem inicial (MFI e a composição botânica e estrutural dos pastos. Para as estimativas de valor nutritivo, foram feitas amostragens simulando o pastejo e analisados a fibra em detergente neutro (FDN, fibra em detergente ácido (FDA, proteína bruta (PB, digestibilidade in vitro da matéria seca (DIVMS e digestibilidade in vitro da matéria orgânica (DIVMO. O delineamento experimental foi o inteiramente casualizado com dois tratamentos e duas repetições, em parcelas subdivididas no tempo. Os valores médios de massa de forragem inicial e da carga animal foram similares entre os SF. Observou-se resultado superior para o teor médio de PB da forragem do SF2. O uso do azevém, das leguminosas e das espécies de crescimento espontâneo em consórcio com o CE permite manter massa de forragem uniforme no decorrer dos pastejos.The objective of this research was to evaluate the productivity and nutritive value of two pasture-based systems (PS with elephant grass (EG + ryegrass (RG + white clover (WC + spontaneous growing species (SGS, for PS1; and EG + RG + forage peanut (FP + SGS, for PS2. EG was established in rows spaced by four meters. Ryegrass was seeding in the space between rows during cool-season and development of SGS was permitted in the warm-season. Holstein cows under rotational grazing were used in the evaluation. The initial forage mass (IFM

  17. Cartilage Degeneration, Subchondral Mineral and Meniscal Mineral Densities in Hartley and Strain 13 Guinea Pigs

    Science.gov (United States)

    Sun, Yubo; Scannell, Brian P; Honeycutt, Patrick R; Mauerhan, David R; H, James Norton; Hanley Jr, Edward N

    2015-01-01

    Osteoarthritis is a joint disease involved in articular cartilage, subchondral bone, meniscus and synovial membrane. This study sought to examine cartilage degeneration, subchondral bone mineral density (BMD) and meniscal mineral density (MD) in male Hartley, female Hartley and female strain 13 guinea pigs to determine the association of cartilage degeneration with subchondral BMD and meniscal MD. Cartilage degeneration, subchondral BMD and meniscal MD in 12 months old guinea pigs were examined with histochemistry, X-ray densitometry and calcium analysis. We found that male Hartley guinea pigs had more severe cartilage degeneration, subchondral BMD and meniscal MD than female Hartley guinea pigs, but not female strain 13 guinea pigs. Female strain 13 guinea pigs had more severe cartilage degeneration and higher subchondral BMD, but not meniscal MD, than female Hartley guinea pigs. These findings indicate that higher subchondral BMD, not meniscal MD, is associated with more severe cartilage degeneration in the guinea pigs and suggest that abnormal subchondral BMD may be a therapeutic target for OA treatment. These findings also indicate that the pathogenesis of OA in the male guinea pigs and female guinea pigs are different. Female strain 13 guinea pig may be used to study female gender-specific pathogenesis of OA. PMID:26401159

  18. Guinea pig complement potently measures vibriocidal activity of human antibodies in response to cholera vaccines.

    Science.gov (United States)

    Kim, Kyoung Whun; Jeong, Soyoung; Ahn, Ki Bum; Yang, Jae Seung; Yun, Cheol-Heui; Han, Seung Hyun

    2017-12-01

    The vibriocidal assay using guinea pig complement is widely used for the evaluation of immune responses to cholera vaccines in human clinical trials. However, it is unclear why guinea pig complement has been used over human complement in the measurement of vibriocidal activity of human sera and there have not been comparison studies for the use of guinea pig complement over those from other species. Therefore, we comparatively investigated the effects of complements derived from human, guinea pig, rabbit, and sheep on vibriocidal activity. Complements from guinea pig, rabbit, and human showed concentration-dependent vibriocidal activity in the presence of quality control serum antibodies. Of these complements, guinea pig complement was the most sensitive and effective over a wide concentration range. When the vibriocidal activity of complements was measured in the absence of serum antibodies, human, sheep, and guinea pig complements showed vibriocidal activity up to 40-fold, 20-fold, and 1-fold dilution, respectively. For human pre- and post-vaccination sera, the most potent vibriocidal activity was observed when guinea pig complement was used. In addition, the highest fold-increases between pre- and post- vaccinated sera were obtained with guinea pig complement. Furthermore, human complement contained a higher amount of V. cholerae- and its lipopolysaccharide-specific antibodies than guinea pig complement. Collectively, these results suggest that guinea pig complements are suitable for vibriocidal assays due to their high sensitivity and effectiveness to human sera.

  19. Comparación de dos métodos para la estimación de los daños por sequía en pastos de dehesa

    OpenAIRE

    Gliga, A.E.; Escribano Rodríguez, J.A.; Hernández Díaz-Ambrona, C.G.

    2012-01-01

    Ponencia presentada a la 51 Reunión Científica de la SEEP celebrada en la Escuela Superior de Ingenieros Agrónomos de la Universidad Pública de Navarra entre el 14 y el 18 de mayo de 2012. El Sistema de Seguros Agrarios con el Seguro de cobertura de los daños por sequía en los pastos aprovechados por el ganado en régimen extensivo (línea de seguro 133) aplica la teledetección mediante un índice de vegetación (NDVI), con el fin de solucionar los problemas de peritación que surgen cuando se ...

  20. Características estruturais do pasto de capim-braquiária de acordo com a localização das fezes Structural characteristics of Brachiaria grass pasture according to the location of feces

    Directory of Open Access Journals (Sweden)

    Manoel Eduardo Rozalino Santos

    2010-10-01

    Full Text Available Objetivou-se avaliar o efeito da localização das fezes de bovinos sobre as características de perfilhos vegetativos e reprodutivos, a massa dos componentes da planta, a densidade volumétrica da forragem e a altura do pasto de Brachiaria decumbens, cv. Basilisk, manejado sob lotação contínua. O delineamento foi em blocos casualizados, com três repetições, em esquema de parcela subdivida, considerando parcelas os locais próximos e distantes das fezes e subparcelas as categorias de perfilhos vegetativos e reprodutivos. A massa de lâmina foliar viva (LFV por perfilho em locais próximos às fezes (0,14 g foi maior que nos locais distantes das fezes (0,08 g, contrariamente ao verificado para a composição de massa de lâmina foliar morta por perfilho. A massa de LFV nos perfilhos vegetativos (0,15 g foi maior que nos perfilhos reprodutivos (0,06 g. Por outro lado, a massa de colmo (0,38 g e de lâmina foliar morta (0,20 g foi maior nos perfilhos reprodutivos. O peso dos perfilhos das plantas próximas às fezes foi maior (0,58 g que daqueles distantes das fezes (0,17 g. Em locais próximos das fezes, o número de perfilhos vegetativos foi menor, enquanto o de perfilhos reprodutivos foi maior. Maiores massas de colmo verde, forragem verde e forragem total do pasto foram observados nos locais próximos das fezes, quando comparadas aos distantes. Mesmo resultado foi obtido para a altura do pasto. A estrutura do pasto de Brachiaria decumbens é modificada pela localização das fezes dos bovinos em pastejo. A deposição de fezes por bovinos constitui fator de heterogeneidade espacial da vegetação em pastagem.The objective of this study was to evaluate the effect of the cattle feces location on the characteristics of vegetative and reproductive tillers, dry mass content in plant components, forage volumetric density and Brachiaria decumbens cv. Basilisk pasture height managed under continuous stocking. It was used a randomized block design

  1. Número e peso de perfilhos no pasto de capim-braquiária sob lotação contínua = Number and weight of tillers in signalgrass pasture under continuous stocking

    Directory of Open Access Journals (Sweden)

    Manoel Eduardo Rozalino Santos

    2011-04-01

    Full Text Available Objetivou-se estudar a relação entre o número e o peso de categorias de perfilhos em pasto de Brachiaria decumbens cv. Basilisk sob lotação contínua com bovinos. O pasto foi manejado com altura média de aproximadamente 25 cm. Foram avaliadas quatro alturas de plantas no mesmo pasto (10, 20, 30 e 40 cm, em delineamento de blocos ao acaso, com duas repetições. Os números de perfilhos vegetativos, vivos e totais reduziram linearmente com a altura da planta. O número de perfilho reprodutivo aumentou de forma linear com a altura da planta (de 8 para 84 perfilhos m-2 quando se comparam plantas de 10 a 40 cm. Os pesos dos perfilhos vegetativos e reprodutivos incrementaram linearmente com a altura da planta. O peso do perfilho vegetativo (0,316 g, em média foi menor do que o peso do perfilho reprodutivo (0,401 g, em média. A relação entre o número e o peso do perfilho vegetativo foi linear e negativa, enquanto que essa relação, para o perfilho reprodutivo, foi linear e positiva. Em locais com plantas altas há menor número de perfilhos vegetativos, bem como maior número de perfilhos reprodutivos e mortos. Nos locais mais altos do pasto de B. decumbens os perfilhos são mais pesados.The experiment was conducted to study the relationship between the number and weight of tiller categories in Brachiaria decumbens cv. Basilisk pasture managed under continuous stocking with cattle. The pasture was managed with an average height of about 25 cm. Plants offour different heights (10, 20, 30 and 40 cm were evaluated by means of the randomized block design with two replications. The number of vegetative tillers, both live and total, reduced linearly according to the plant height. The number of reproductive tillers increased linearly according to pasture plant height (from 8 to 84 tillers m-2 when comparing plants 10 to 40 cm high. Vegetative and reproductive tiller weight increased linearly according to pasture plant height. Vegetative tiller

  2. Caracterização dos perfilhos em pastos de capim-braquiária diferidos e adubados com nitrogênio Tiller characteristics in nitrogen fertilized and deferred signalgrass pastures

    Directory of Open Access Journals (Sweden)

    Manoel Eduardo Rozalino Santos

    2009-04-01

    Full Text Available Este trabalho foi conduzido com o objetivo de avaliar o perfilhamento e as características estruturais de perfilhos em pastos de Brachiaria decumbens cv. Basilisk sob três períodos de diferimento da pastagem (73, 95 e 116 dias e quatro doses de nitrogênio (N (0, 40, 80 e 120 kg/ha. O delineamento experimental foi em blocos casualizados, com três repetições, em esquema de parcelas subdivididas. O número de perfilhos vegetativos (PV reduziu com o aumento do período de diferimento. Houve interação entre período de diferimento e dose de N para a densidade populacional de PV. O maior período de diferimento elevou o número de perfilhos reprodutivos (PR nos pastos de capim-braquiária, mas não houve efeito da dose de N sobre o número de perfilhos reprodutivos. O número de perfilhos mortos não foi influenciado pelos fatores estudados. Tanto o período de diferimento quanto a adubação nitrogenada aumentaram o peso de todas as categorias de perfilhos de capim-braquiária. As características estruturais dos perfilhos presentes nos pastos diferidos foram alteradas pelo período de diferimento e pela dose de nitrogênio. A redução do período de diferimento e a adubação nitrogenada são estratégias de manejo adequadas para aumentar o número de perfilhos vegetativos em pastos de Brachiaria decumbens cv. Basilisk.This work aimed to evaluate the tilling and tiller structural characteristics on Brachiaria decumbens cv. Basilisk pasture fertilized with nitrogen (N under different deferring periods. The treatments had 73, 95 and 116-day deferring periods, and 0, 40, 80 and 120 kg/ha N doses. A randomized block design with three replicates and subdivided plots was used. The number of vegetative tillers (VT reduced as the deferring period increased. Interaction between the deferring period and N doses on the VT population density was verified. The longest deferring period increased the number of reproductive tillers (RT on the B. decumbens

  3. [Management of ectopic pregnancy in Conakry, Guinea].

    Science.gov (United States)

    Sy, T; Diallo, Y; Toure, A; Diallo, F B; Balde, A A; Hyjazi, Y; Diallo, M S

    2009-12-01

    Ectopic pregnancy is one of the most frequent hemorrhagic emergencies encountered in gynecology and obstetrics. The purpose of this 16-month descriptive prospective study at the Ignace Deen Gynecology-Obstetric clinic at Conakry University Hospital in Guinea was to assess diagnostic techniques and therapeutic attitudes regarding ectopic pregnancy in a low-resource setting. The frequency of ectopic pregnancy was 1.4%. Mean patient age was 28.9 years. Ectopic pregnancy was often observed at the second or third pregnancy (47.1%) in women who were giving birth for the second or third time (36.0%) and had a history of sexually transmitted infections (88.2%) or abortions (43.1%). Most women had no schooling (60.8 %), were poor and lived in a marital home (86.3%). Presenting symptoms included the classic triad of amenorrhea (98.0%), abdominopelvic pain (92.2%), and vaginal bleeding (62.7%). Definitive diagnosis was achieved by ultrasound examination in 76.6% of cases and by puncture of the Douglas pouch in 84%. The most frequent site of ectopic pregnancy was the ampulla of the uterine tube (66.9%). Abdominal and ovarian pregnancy was observed in 3 and 4 of the 51 cases respectively. Surgical management was performed in all cases. The most frequent procedure was salpingectomy (80.3%). Proper treatment of sexually transmitted infections (STI), start-up of post-abortion care facilities, and provision of information during early consultation at the first signs of pregnancy would help reduce the frequency and improve the prognosis of ectopic pregnancy.

  4. Ebola Surveillance - Guinea, Liberia, and Sierra Leone.

    Science.gov (United States)

    McNamara, Lucy A; Schafer, Ilana J; Nolen, Leisha D; Gorina, Yelena; Redd, John T; Lo, Terrence; Ervin, Elizabeth; Henao, Olga; Dahl, Benjamin A; Morgan, Oliver; Hersey, Sara; Knust, Barbara

    2016-07-08

    Developing a surveillance system during a public health emergency is always challenging but is especially so in countries with limited public health infrastructure. Surveillance for Ebola virus disease (Ebola) in the West African countries heavily affected by Ebola (Guinea, Liberia, and Sierra Leone) faced numerous impediments, including insufficient numbers of trained staff, community reticence to report cases and contacts, limited information technology resources, limited telephone and Internet service, and overwhelming numbers of infected persons. Through the work of CDC and numerous partners, including the countries' ministries of health, the World Health Organization, and other government and nongovernment organizations, functional Ebola surveillance was established and maintained in these countries. CDC staff were heavily involved in implementing case-based surveillance systems, sustaining case surveillance and contact tracing, and interpreting surveillance data. In addition to helping the ministries of health and other partners understand and manage the epidemic, CDC's activities strengthened epidemiologic and data management capacity to improve routine surveillance in the countries affected, even after the Ebola epidemic ended, and enhanced local capacity to respond quickly to future public health emergencies. However, the many obstacles overcome during development of these Ebola surveillance systems highlight the need to have strong public health, surveillance, and information technology infrastructure in place before a public health emergency occurs. Intense, long-term focus on strengthening public health surveillance systems in developing countries, as described in the Global Health Security Agenda, is needed.The activities summarized in this report would not have been possible without collaboration with many U.S and international partners (http://www.cdc.gov/vhf/ebola/outbreaks/2014-west-africa/partners.html).

  5. Potassium transport across guinea pig distal colon

    International Nuclear Information System (INIS)

    Rechkemmer, G.; Halm, D.R.; Frizzell, R.A.

    1986-01-01

    Active absorption and secretion of K was studied by measuring bidirectional 42 K fluxes across short-circuited guinea pig distal colon. Tissues were pretreated with mucosal (m) and serosal (s) indomethacin (1 μM) and amiloride (0.1 mM, m) to suppress spontaneous, electrogenic Cl secretion and Na absorption. Under these conditions, the short-circuit current (I/sub sc/) was 0.4 μeq/cm 2 h while electroneutral K absorption was 2.8 μeq/cm 2 h. Epinephrine (5 μM, s) stimulated electrogenic K secretion, reducing net K absorption to 1.3 μeq/cm 2 h. Bumetanide (0.1 mM, s) abolished this K secretion and restored K absorption to control values, suggesting mechanistic similarities between K and Cl secretion. K absorption was inhibited 40% by the gastric H/K ATPase inhibitor, omeprazole (0.1 mM, m), and was abolished by ouabain (0.1 mM, m). Neutral K absorption does not appear to be mediated by an apical membrane Na/K pump since: the effect of mucosal ouabain on K absorption does not require the presence of mucosal or serosal Na, unidirectional Na fluxes are not influenced by mucosal ouabain, and K absorption is not affected when Na absorption is abolished by amiloride. Net K transport is determined by the balance between electroneutral K absorption and electrogenic K secretion. The ouabain sensitivity of K absorption suggests that colonic H/K ATPase differs from its gastric counterpart

  6. Infection of guinea pigs with vesicular stomatitis New Jersey virus Transmitted by Culicoides sonorensis (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Pérez De León, Adalberto A; O'Toole, Donal; Tabachnick, Walter J

    2006-05-01

    Intrathoracically inoculated Culicoides sonorensis Wirth & Jones were capable of transmitting vesicular stomatitis New Jersey virus (family Rhabdoviridae, genus Vesiculovirus, VSNJV) during blood feeding on the abdomen of six guinea pigs. None of the guinea pigs infected in this manner developed clinical signs of vesicular stomatitis despite seroconversion for VSNJV. Guinea pigs infected by intradermal inoculations of VSNJV in the abdomen also failed to develop clinical signs of vesicular stomatitis. Three guinea pigs given intradermal inoculations of VSNJV in the foot pad developed lesions typical of vesicular stomatitis. Transmission by the bite of C. sonorensis may have facilitated guinea pig infection with VSNJV because a single infected C. sonorensis caused seroconversion and all guinea pigs infected by insect bite seroconverted compared with 50% of the guinea pigs infected by intradermal inoculation with a higher titer VSNJV inoculum. The role of C. sonorensis in the transmission of VSNJV is discussed.

  7. La capacitación como elemento clave del modelo para la difusión y adopción de tecnologías de semillas en el sector ganadero cubano (Nota técnica

    Directory of Open Access Journals (Sweden)

    Marlen Navarro

    Full Text Available Se desarrolló un trabajo orientado hacia la identificación participativa de las necesidades de capacitación y la construcción de un proceso de aprendizaje horizontal, como base para la adopción y difusión de las tecnologías de semillas de pastos. Para valorar los factores que influyen en la adopción de dichas tecnologías se realizaron talleres locales, en los que se elaboró el árbol de problemas y el de objetivos para cada una de los problemas identificados. Con esta información y con la obtenida en los intercambios con productores de los sectores formal e informal, se diseñó el curso teórico-práctico «Producción y tecnologías de semillas de pastos tropicales». La importancia de la adquisición y profundización de conocimientos se valoró a través de los aspectos «comprobación de conocimientos» y «proyecto de perspectivas y desarrollo»; mientras que la evolución del proceso de capacitación se analizó en tres proyectos, de los presentados por cada una de las fincas en fomento. A través de la capacitación participativa se logró que los implicados en la adopción y difusión de las tecnologías de semillas de pastos propusieran los temas en que se debía profundizar

  8. Resisting State Iconoclasm Among the Loma of Guinea

    DEFF Research Database (Denmark)

    Højbjerg, Christian Kordt

    -term suppression, including the exposure of secrecy, by the postcolonial authorities. In recent years the famous male ritual association known as Poro has even taken on new significance in the context of political upheaval in the war-torn border area between Guinea and Liberia. Drawing on extensive fieldwork...

  9. Cytological Determination Of The Estrus Cycle In Guinea Pigs ...

    African Journals Online (AJOL)

    A cytologic study was carried out to determine the Estrus cycle in 5 female guinea pigs of reproductive age, for a period of 28 days in the Animal House of the College of Medicine, University of Nigeria, Enugu Campus, Enugu. Vaginal smears were collected from the animals and made on clean grease-free slides, fixed in ...

  10. Survey of endoparasites in pet guinea pigs in Italy.

    Science.gov (United States)

    d'Ovidio, Dario; Noviello, Emilio; Ianniello, Davide; Cringoli, Giuseppe; Rinaldi, Laura

    2015-03-01

    Little information is available on the occurrence of endoparasites in pet guinea pigs (Cavia porcellus). The purpose of this study was to evaluate the prevalence of intestinal parasites in cavies kept as pets in southern Italy. Fresh fecal samples were randomly collected from 60 guinea pigs housed in pet shops or privately owned. All fecal samples were processed using the FLOTAC pellet technique to identify and count helminthic eggs/larvae and protozoan cysts/oocysts. In addition, the specimens were analyzed also by the Remel Xpect® Giardia/Cryptosporidium immunoassay. Intestinal parasites were detected in 19 out of 60 guinea pigs (31.7 %). Paraspidodera uncinata eggs were found in 13.3 % (8/60) of the rodents examined, Nippostrongylus-like eggs in 10 % (6/60), and finally Eimeria caviae oocysts were found in 10 % (6/60) of the animals. In one case, both E. caviae oocysts and P. uncinata eggs were found. None of the samples was positive for Cryptosporidium or Giardia. To the authors' knowledge, this is the first survey of endoparasites in pet guinea pigs in Italy.

  11. Composition and characteristics of urinary calculi from guinea pigs.

    Science.gov (United States)

    Hawkins, Michelle G; Ruby, Annette L; Drazenovich, Tracy L; Westropp, Jodi L

    2009-01-15

    To determine the mineral composition of calculi, anatomic locations of the calculi, and findings of urinalysis and bacteriologic culture of urine and calculi in guinea pigs with urolithiasis. Cross-sectional study. 127 guinea pigs. Records of urinary calculi that had been submitted to the University of California Stone Laboratory from 1985 through 2003 were reviewed. In addition, submissions of urinary calculi for evaluation by the laboratory were prospectively solicited from 2004 through 2007. Prospectively obtained calculi were accompanied by a urine sample for urinalysis and bacteriologic culture and a completed questionnaire. All calculi were analyzed by use of polarized light microscopy and infrared spectroscopy. A subset of calculi was examined by means of x-ray diffractometry (XRD). 83% (43/52) of calculi from the laboratory database and 93% (70/75) of calculi that were prospectively solicited were composed of 100% calcium carbonate. Analysis via XRD confirmed that 5 of 6 calculi from a subset that had the greatest gross morphologic variation were composed of 100% calcite. Although many guinea pigs had received anti-microbials before bacteriologic cultures of urine were performed, Corynebacterium renale was isolated from 5 urine samples. Contrary to findings of other studies, urinary calculi analyzed for the present study were most commonly composed of 100% calcium carbonate, and infrared spectroscopy or XRD was necessary to differentiate this mineral from others. Treatments, including diet and husbandry practices, should be developed to help prevent development of calcium carbonate calculi in guinea pigs.

  12. A new fish of the family Scorpaenidae from New Guinea

    NARCIS (Netherlands)

    Mees, G.F.

    1964-01-01

    The ichthyological collections brought together in various parts of New Guinea by staff members of the Rijksmuseum van Natuurlijke Historie in 1954 and 1955 (cf. Boeseman, 1963), contain a series of scorpion-fishes which are here described as a new species, representing a new genus. Cheroscorpaena

  13. Plague in Guinea Pigs and Its Prevention by Subunit Vaccines

    Science.gov (United States)

    Quenee, Lauriane E.; Ciletti, Nancy; Berube, Bryan; Krausz, Thomas; Elli, Derek; Hermanas, Timothy; Schneewind, Olaf

    2011-01-01

    Human pneumonic plague is a devastating and transmissible disease for which a Food and Drug Administration–approved vaccine is not available. Suitable animal models may be adopted as a surrogate for human plague to fulfill regulatory requirements for vaccine efficacy testing. To develop an alternative to pneumonic plague in nonhuman primates, we explored guinea pigs as a model system. On intranasal instillation of a fully virulent strain, Yersinia pestis CO92, guinea pigs developed lethal lung infections with hemorrhagic necrosis, massive bacterial replication in the respiratory system, and blood-borne dissemination to other organ systems. Expression of the Y. pestis F1 capsule was not required for the development of pulmonary infection; however, the capsule seemed to be important for the establishment of bubonic plague. The mean lethal dose (MLD) for pneumonic plague in guinea pigs was estimated to be 1000 colony-forming units. Immunization of guinea pigs with the recombinant forms of LcrV, a protein that resides at the tip of Yersinia type III secretion needles, or F1 capsule generated robust humoral immune responses. Whereas LcrV immunization resulted in partial protection against pneumonic plague challenge with 250 MLD Y. pestis CO92, immunization with recombinant F1 did not. rV10, a vaccine variant lacking LcrV residues 271-300, elicited protection against pneumonic plague, which seemed to be based on conformational antibodies directed against LcrV. PMID:21406168

  14. A general measles vaccination campaign in urban Guinea-Bissau

    DEFF Research Database (Denmark)

    Byberg, S.; Thysen, S. M.; Rodrigues, A.

    2017-01-01

    Background Measles vaccination campaigns targeting children aged 9–59 months are conducted every three years in Guinea-Bissau. Studies have demonstrated beneficial non-specific effects of measles vaccine. We compared mortality one year after the December 2012 measles vaccination campaign in Bissa...

  15. Reflections on the Fiftieth Reunion of the Guinea Pigs.

    Science.gov (United States)

    Loud, Oliver

    1988-01-01

    A member of the original faculty of the experimental Ohio State University Laboratory High School reflects at a fiftieth reunion of the first graduating class. Students were used as guinea pigs to determine the effects of providing teenagers with liberating, interesting, and customized education from university faculty. (SM)

  16. A new ostracod (Entocytheridae, Notocytherinae) on New Guinea crayfish1)

    NARCIS (Netherlands)

    Hart, D.G.; Hart, C.W.

    1970-01-01

    In 1967 we examined the crayfish collections in the Rijksmuseum van Natuurlijke Historie, Leiden, to determine if the crayfishes collected in New Guinea in 1954 by Dr. L. B. Holthuis, Dr. L. D. Brongersma, and Dr. M. Boeseman (Boeseman, 1963) were hosts to commensal ostracods. Of the collections

  17. Petroleum scene heating in fledgling crude exporter Papua New Guinea

    International Nuclear Information System (INIS)

    Anon.

    1994-01-01

    Operators, paced by a feisty independent based in Port Moresby, have drilled a string of discoveries near the infrastructure of the Kutubu development project that supports Papua New Guinea crude exports. All signs point to the increasing likelihood of good sized -- maybe world class -- oil discoveries that promise to sustain exploration and development interest beyond 2000. Also in the offing are world class gas strikes that eventually could support a liquefied natural gas export project. And integration is the newest concept in Papua New Guinea petroleum. Efforts are under way to build the country's first refineries. Most operators in Papua New Guinea believe thy have merely scratched the surface of the country's oil and gas potential. Thy agree there still will be frustrations and setbacks -- political as well as technical -- but the prevailing opinion is that these problems are no greater than they are in a number of other countries with similar exploration/development potential. The paper discusses the development of Papua New Guinea's oil and gas industry, and exploratory drilling in areas other than Kutubu

  18. Identity and Ethnomathematics Projects in Papua New Guinea

    Science.gov (United States)

    Owens, Kay

    2012-01-01

    Indigenous students may find mathematics in schools difficult because there is discontinuity between cultural mathematics and school mathematics. One of the reasons for this is that their teacher's identity as a mathematical thinker may not link to their cultural ways of thinking. In Papua New Guinea, there is a subject to assist student teachers…

  19. Five new species of Barringtonia (Lecythidaceae) from Papua New Guinea

    NARCIS (Netherlands)

    Jebb, M.; Prance, G.T.

    2011-01-01

    Five new species of Barringtonia from Papua New Guinea are described and discussed: B. lumina, B. monticola, B. pinnifolia, B. serenae and B. tagala. All five species belong to section Barringtonia with closed flower buds. Notes are also provided for the seven monocaulous pachycaul species of

  20. Folk Opera: Stories Crossing Borders in Papua New Guinea

    Science.gov (United States)

    Haseman, B.; Baldwin, A.; Linthwaite, H.

    2014-01-01

    The Life Drama project is a drama-based sexual health promotion project, developed by a cross-cultural research team in Papua New Guinea (PNG) over the past four years. Recognising the limitations of established theatre-in-education and theatre-for-development approaches when working across cultures, the research team explored ways of tapping into…

  1. Mother Tongue-Based Bilingual Education in Papua New Guinea

    Science.gov (United States)

    Malone, Susan; Paraide, Patricia

    2011-01-01

    Papua New Guinea (PNG), an independent state in the southwest Pacific, is the most linguistically diverse country in the world. Its roughly six million people speak over 800 distinct languages. In spite of this diversity, in 1995 the Papua New Guinean government established a mother tongue-based bilingual education programme in which community…

  2. Dracunculus medinensis (Guinea worm disease): a rare cause of calcification

    Energy Technology Data Exchange (ETDEWEB)

    Gospos, C.

    1980-01-01

    Tangled whorly calcifications were seen in the abdominal subcutaneous tissues of a negro patient from Africa. The differential diagnosis of such calcifications - rarely observed in Europe - includes a variety of parasites. In this patient, Dracunculus medinensis (guinea worm disease) was the cause.

  3. Remuneration disparities in Oceania: Papua New Guinea and Solomon Islands.

    Science.gov (United States)

    Marai, Leo; Kewibu, Vincent; Kinkin, Elly; Peter Peniop, John; Salini, Christian; Kofana, Genesis

    2010-10-01

    This paper explores the impact of remuneration differences on workers in the Solomon Islands and Papua New Guinea. In these countries remunerative differences are linked to government policy (in Papua New Guinea) and job contracts (in the Solomon Islands), and have impacted on industrial relations in both settings (strike action). A total of N = 350 professionals (n = 60 expatriates) from 54 organizations in aid, government, higher education and industry (mean response rate = 36%) responded to an organizational survey form. Remuneration ratios between international and local respondents based on the World Bank's index of purchasing power parity approached 9:1. In both sites staff compared pay and benefits (remuneration) packages: Internationally remunerated staff rated their ability higher than their local counterparts did; locally remunerated groups reported more injustice in remuneration, were more demotivated by the gaps, and were more likely to be thinking about leaving the organization. In-country workshops of N = 40 largely local stakeholders from aid and community organizations plus government ministries considered the survey's findings and recommended: in Solomon Islands, (a) introducing a policy of localization, (b) establishing a remuneration commission (already existent in Papua New Guinea), and (c) reducing the remunerative gap; in Papua New Guinea, (d) reversing the post-Independence "dual pay system" (currently official policy), (e) instituting pay-for-performance, and (f) ensuring the existent localization policy is applied to recruitment, selection, and staff career planning and management.

  4. Evaluation of community-based surveillance for Guinea worm, South ...

    African Journals Online (AJOL)

    2012-08-03

    Aug 3, 2012 ... deleted at the Data Manager Level in Loki. Conclusion. Community-based surveillance for guinea worm is a good example of a surveillance system on which an integrated disease surveillance system can be based in countries with poor surveillance like South Sudan. This makes its potential value to ...

  5. Notes on New Guinea Rubiaceae. Versteegia and Maschalodesme

    NARCIS (Netherlands)

    Ridsdale, C.E.; Bakhuizen van den Brink, R.C.; Koek-Noorman, J.

    1972-01-01

    Within the rain forests of New Guinea there are many small pachycaul treelets belonging to the Rubiaceae. Generally these are rare in occurrence and poorly represented in the herbarium, due in part to the problem of protecting rami- or cauliflorous flowers and fruits during routine processing and

  6. The Implementation of Curricular Reform: Tanzania and Papua New Guinea.

    Science.gov (United States)

    Saunders, Murray; Vulliamy, Graham

    1983-01-01

    Discusses the origins and policy characteristics of the Education for Self Reliance project in Tanzania and the Secondary Schools Community Extension Project in Papua New Guinea. Compares the sociopolitical climate, the policy characteristics, the role of academic assessment, policy change, and examples of the existing policy in the two countries.…

  7. Notes on two small collections of Birds from new Guinea

    NARCIS (Netherlands)

    Mees, G.F.

    1964-01-01

    INTRODUCTION Over the past few years the Rijksmuseum van Natuurlijke Historie has received, from different sources, several small collections of birds from various parts of New Guinea. This is a report on two of these. No ornithologist accompanied the expedition to the Sterren-gebergte (Star

  8. Piper (Piperaceae) in New Guinea: the non-climbing species

    NARCIS (Netherlands)

    Gardner, R.O.

    2003-01-01

    A taxonomic account is given of six Piper species of New Guinea: P. bolanicum spec. nov., P. gibbil­imbum, P. recessum spec. nov., P. subbullatum, P. triangulare and P. wabagense. These small shrubby trees are best represented in secondary growth and forest at 1300–2500 m altitude, with P.

  9. Effects of Perfluoroisobutene on Guinea Pig Isolated Lung Preparations.

    Science.gov (United States)

    guinea pigs showed increased sensitivity to 5-hydroxytryptamine, bradykinin, isoprenaline and a thromboxane A2 analogue, but not to carbachol, histamine, prostaglandin D2, adenosine or potassium. These changes were not detected in the TS. If PFIB-induced changes in lung pharmacology contribute to oedema, antagonists of identified mediators may be useful as pretreatments/therapies to pulmonary

  10. Seasonal variation in child mortality in rural Guinea-Bissau

    DEFF Research Database (Denmark)

    Nielsen, Bibi Uhre; Byberg, Stine; Aaby, Peter

    2017-01-01

    Objectives: In many African countries, child mortality is higher in the rainy season than in the dry season. We investigated the effect of season on child mortality by time periods, sex and age in rural Guinea-Bissau. Methods: Bandim health project follows children under-five in a health...

  11. Feeding ecology of wintering terns in Guinea-Bissau

    NARCIS (Netherlands)

    Brenninkmeijer, A; Stienen, EWM; Klaassen, M; Kersten, M.

    2002-01-01

    We studied the feeding ecology of Little Terns Sterna albifrons , Sandwich Terns S. sandvicensis and Royal Terns S. maxima in the Archipelago dos Bijagos (11degrees40'N, 15degrees45'W) in Guinea-Bissau (West Africa) during the winter of 1992/1993. More than 95% of all prey taken by these terns were

  12. Guinea pig ductus arteriosus. II - Irreversible closure after birth.

    Science.gov (United States)

    Fay, F. S.; Cooke, P. H.

    1972-01-01

    To investigate the mechanism underlying irreversibility of ductal closure after birth, studies were undertaken to determine the exact time course for the onset of irreversible closure of the guinea pig ductus arteriosus. Parallel studies of the reactivity of ductal smooth muscle to oxygen and studies of the postpartum cellular changes within the vessel were also carried out.

  13. Perilymphatic and endolymphatic pressure in the normal guinea pig

    NARCIS (Netherlands)

    Warmerdam, TJ; Schroder, FHH; Wit, HP; Albers, FWJ

    1999-01-01

    Perilymphatic and endolymphatic pressures were measured consecutively in the normal guinea pig using a WPI 900A micropressure system. The mean (n = 10) perilymphatic and endolymphatic pressures were 2.26 and 2.31 cm H2O, respectively. There was no statistically significant difference between the

  14. Cost and Return Analysis of Ginger Production in the Guinea ...

    African Journals Online (AJOL)

    The study focuses on the cost and return analysis of ginger production in the guinea savannah agro ecology of Nigeria. The study is aimed at highlighting the profitability of ginger production with a view to informing and influencing farmers and relevant stakeholders. The study was conducted at Kajuru, Kaduna state in the ...

  15. Guinea worm eradication program in Borno state: The need for ...

    African Journals Online (AJOL)

    The objective of this study is to update the status of Guinea worm and Eradication Program in Borno State and to demonstrate the effectiveness of adopting appropriate intervention strategies. The assessment of the prevalence of Dracunculus medinensis was carried out by active case search. Three hundred and ten ...

  16. Prevalence And Disabilities Caused By Guinea-Worm Disease ...

    African Journals Online (AJOL)

    A survey was carried out in 2001 to ascertain the status of Guinea worm disease infection among farm households in Ebonyi Local Government Area (LGA) of Ebonyi State. A total of 3,777 respondents were randomly sampled from 15 communities that comprised the LGA. The sample respondents were clinically examined ...

  17. Absorption of transuranic nitrates by rats, guinea pigs, and dogs

    International Nuclear Information System (INIS)

    Sullivan, M.F.

    1978-01-01

    Absorption of 238 Pu and 241 Am from the gstrointestinal tract was found to be quite similar for rats, guinea pigs, and dogs. Different oxidation state mixtures of 238 Pu nitrate (95% versus 65% Pu IV) were absorbed to about the same extent

  18. The alpine flora of Mount Wilhelm (New Guinea)

    NARCIS (Netherlands)

    Hoogland, R.D.

    1958-01-01

    The flora of the higher mountains of New Guinea has been the object of several extensive collecting trips in the past forty years. Until quite recently, however, a serious gap in our knowledge was the very scanty information available from the area between Mount Wilhelmina in the West and Mount

  19. Petroleum service projects in the Gulf of Guinea

    Science.gov (United States)

    Ken-Worgu, Kenneth Chukwumeka

    2011-07-01

    The goal of this record of study is to examine the major facets involved in managing several petroleum service projects located in three different countries in the Gulf of Guinea simultaneously, while effectively engaging in business development activities for the Oil and Industrial Services Group (OIS). This work also furnishes adequate background on related subject matters to enable understanding of the projects presented. The petroleum services sector is the back bone of the oil and gas industry. Services companies are vital to the success of all petroleum and energy producers in the USA, the Gulf of Guinea and the world. There is a need and demand for these service companies because they play various roles such as logistics, drilling, construction, dredging, pipe laying, procurement, food supply, human resource supply, etc. The Gulf of Guinea comprises of countries from west and central Africa. This project was limited to Nigeria, Equatorial Guinea and Cameroon. This area holds the largest petroleum reserves in Africa and plays a vital role in the global supply of petroleum. The Oil and Industrial Services Group (OIS), plans to establish herself as one of the leading petroleum service companies in this gulf. To manage this expansion, I have taken the role of Gulf of Guinea manager to apply my background as a petroleum engineer as well as my business skills to build a successful division of the company. This work provides a record of study of the management of services, projects and contracts carried out by the OIS group in the gulf of Guinea. The following are the specific projects in the Gulf of Guinea that I participated in: Managing delivering, maintenance and marketing of offshore vessels, Offshore pipe laying project, Integrated pipeline maintenance project, Development a petroleum technical training facilities, Agbami pipe insulation project, Engineering lift project and Capital budgeting analysis for potential investments. The details of the specific

  20. Toluene-induced hearing loss in the guinea pig.

    Science.gov (United States)

    Waniusiow, Delphine; Campo, Pierre; Venet, Thomas; Cossec, Benoît; Cosnier, Frédéric; Beydon, Dominique; Rieger, Benoît; Burgart, Manuella; Ferrari, Luc; Parietti-Winkler, Cécile

    2009-10-01

    Toluene is a high-production industrial solvent, which can disrupt the auditory system in rats. However, toluene-induced hearing loss is species dependent. For instance, despite long-lasting exposures to high concentrations of aromatic solvent, no study has yet succeeded in causing convincing hearing loss in the guinea pig. This latter species can be characterized by two metabolic particularities: a high amount of hepatic cytochrome P-450s (P-450s) and a high concentration of glutathione in the cochlea. It is therefore likely that the efficiency of both the hepatic and cochlear metabolisms plays a key role in the innocuousness of the hearing of guinea pigs to exposure to solvent. The present study was carried out to test the auditory resistance to toluene in glutathione-depleted guinea pigs whose the P-450 activity was partly inhibited. To this end, animals on a low-protein diet received a general P-450 inhibitor, namely SKF525-A. Meanwhile, they were exposed to 1750 ppm toluene for 4 weeks, 5 days/week, 6 h/day. Auditory function was tested by electrocochleography and completed by histological analyses. For the first time, a significant toluene-induced hearing loss was provoked in the P-450-inhibited guinea pigs. However, the ototoxic process caused by the solvent exposure was different from that observed in the rat. Only the stria vascularis and the spiral fibers were disrupted in the apical coil of the cochlea. The protective mechanisms developed by guinea pigs are discussed in the present publication.

  1. Vestido, identidad y folklore. La invención de un vestido nacional de Guinea Ecuatorial

    Directory of Open Access Journals (Sweden)

    Valenciano-Mañé, Alba

    2012-06-01

    Full Text Available Focussing on clothing viewed as a body practice, this paper attempts to provide an ethnographic overview through the examination of the creation of the national dress of Equatorial Guinea. With a discussion of the importance of the personal motivation of its promoters, the reasons for its alleged failure and the recent attempts in current fashion shows in Malabo to (reproduce identity discourses, this paper also seeks to demonstrate the capacity of dress as a tool for social control, resistance and identity (recreation.

    Situando el vestido como práctica corporal en el centro de la reflexión, el artículo efectúa un recorrido etnográfico por el proceso de creación de un vestido nacional de Guinea Ecuatorial. Se presentará la importancia de la ecuación personal de sus impulsores, los motivos de su fracaso y las recientes tentativas de (reproducción de discursos identitarios en los actuales fashion shows de Malabo. De este modo, se pretende demostrar el potencial del vestido como herramienta de control social, resistencia y (recreación identitaria.

  2. The Changing Face of Librarianship in Papua New Guinea: Libraries for Life in the Papua New Guinea Information Society?

    Science.gov (United States)

    Obi, Margaret J.

    "Libraries for life" in Papua New Guinea today is not an impossible goal to strive for to achieve with today's new and old information and communication technologies. However, in order for this to happen, a number of questions will need to be asked. There are three that need immediate attention: (1) What is an "information…

  3. Potencial del Agua del suelo

    Directory of Open Access Journals (Sweden)

    Bustamante Heliodoro

    1986-12-01

    Full Text Available La energía potencial del agua presenta diferencias de un punto del suelo a otro; esas diferencias son las que originan el movimiento del agua de acuerdo a la tendencia universal de la materia en el sentido de moverse de donde la energía potencial es mayor a donde dicha energía es menor. En el suelo el agua en consecuencia se mueve hacia donde su energía decrece hasta lograr su estado de equilibrio. Se desprende entonces que la cantidad de energía potencial absoluta contenida en el agua, no es importante por sí misma, sino por su relación con la energía en diferentes lugares dentro del suelo. El concepto Potencial de agua del suelo es un criterio para esta energía.

  4. Validación del modelo expo-lineal precipitación-escurrimiento en un simulador de lluvia

    Directory of Open Access Journals (Sweden)

    Fernando Paz

    2017-10-01

    Full Text Available La necesidad de desarrollar métodos simplificados, operativos y coherentes para modelar la relación precipitación-escurrimiento a escala de eventos, permanece como uno de los grandes retos en el campo de la hidrología. Si bien existen propuestas simples de modelación como el método del Número de Curva o NC, sus bases teóricas no tienen fundamentos hidrológicos. La ventaja del método del NC es que solo requiere de un parámetro, lo cual lo hace muy atractivo en las aplicaciones. En este trabajo se analizan las bases del método del NC. Los resultados muestran que estos intentos simplificados de modelación están limitados. Por otro lado, el modelo expo-lineal de Paz (2013 es flexible en términos empíricos. Las bases teóricas de este modelo son discutidas en términos de la hidrología de área variable, en un formato suficientemente general para hacer solido su uso. El modelo se aplicó a un experimento en un simulador de lluvias con lotes de escurrimiento con diferentes tipos de suelo y coberturas aéreas de pastos. Los resultados mostraron un buen ajuste experimental a los datos, particularmente en términos de parametrizar el modelo expo-lineal en forma similar al método del NC. De estos desarrollos, se presenta un esquema de estimación de los parámetros del modelo expo-lineal que se puede usar en forma equivalente, por su simplicidad al tener un solo parámetro libre, al método del NC.

  5. Subacute Low Dose Nerve Agent Exposure Causes DNA Fragmentation in Guinea Pig Leukocytes

    Science.gov (United States)

    2005-10-01

    1 SUBACUTE LOW DOSE NERVE AGENT EXPOSURE CAUSES DNA FRAGMENTATION IN GUINEA PIG LEUKOCYTES. Jitendra R. Dave1, John R. Moffett1, Sally M...DNA fragmentation in blood leukocytes from guinea pigs by ‘Comet’ assay after exposure to soman at doses ranging from 0.1LD50 to 0.4 LD50, once per...computer. Data obtained for exposure to soman demonstrated significant increases in DNA fragmentation in circulating leukocytes in CWNA treated guinea pigs as

  6. Immunohistopathology in the Guinea Pig Following Chronic Low-Level Exposure to Chemical Warfare Agents

    Science.gov (United States)

    2005-11-01

    U.S. Army Medical Research Institute of Chemical Defense USAMRICD-TR-05-09 Immunohistopathology in the Guinea Pig Following Chronic Low...2005 2. REPORT TYPE Technical Report 3. DATES COVERED (From - To) May 2003 to April 2005 4. TITLE AND SUBTITLE Immunohistopathology in the Guinea Pig Following...release; distribution unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT Guinea pigs exposed repeatedly to low levels of chemical warfare nerve agents

  7. Efficacy of the Tertiary Oxime Monoisonitrosoacetone (MINA) Against Lethal Sarin Intoxication in the Guinea Pig

    Science.gov (United States)

    2007-10-01

    Sarin 5a. CONTRACT NUMBER Intoxication in the Guinea Pig 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Koplovitz, I and...efficacy of MINA as a treatment for lethal sarin (GB) intoxication in guinea pigs . Male animals were challenged subcutaneously (s.c.) with 2 LD50s...oximes that are readily able to enter the brain. 15. SUBJECT TERMS oximes, brain, sarin, reactivation, nerve agents, guinea pigs 16. SECURITY

  8. The Potential Neurotoxic Effects of Low-Dose Sarin Exposure in a Guinea Pig Model

    Science.gov (United States)

    2002-01-01

    1 THE POTENTIAL NEUROTOXIC EFFECTS OF LOW-DOSE SARIN EXPOSURE IN A GUINEA PIG MODEL Melinda R. Roberson, PhD, Michelle B. Schmidt...Proving Ground, MD 21010 USA ABSTRACT This study is assessing the effects in guinea pigs of repeated low-dose exposure to the nerve...COVERED - 4. TITLE AND SUBTITLE The Potential Neurotoxic Effects Of Low-Dose Sarin Exposure In A Guinea Pig Model 5a. CONTRACT NUMBER 5b

  9. In Vitro Response of Guinea Pig Peritoneal Macrophages to Legionella pneumophila

    Science.gov (United States)

    1981-03-01

    In Vitro Responlse of Guinea Pig Peritoneal Macrophages to Legionella pneumophila It. A. KISIIIMi~O~’ .1. Ii.,W11ITE, F. G. SIREY, V. (U.1 Mc(GANN, R...Tlhe inter- act ion between L. lincumnophila andl( peritoneal macrophages front normial guinea pigs or front animials that had survived infection was...roagglujtiniat titer) guinea pig serum to The purp~ose of this study "as to investigate contain approximately 108 organisms per ml. the phagocytic and

  10. Guinea pig Ia antigens are not derivatised on trinitrophenyl-modified cells

    Energy Technology Data Exchange (ETDEWEB)

    Clement, L T; Thomas, D W; Kask, A M; Shevach, E M [National Inst. of Allergy and Infectious Diseases, Bethesda, MD (USA)

    1978-08-10

    In an effort to clarify the nature of the trinitrophenyl (TNP) specific immunogen recognised by hapten-reactive guinea pig T lymphocytes, the membrane proteins of TNP-modified guinea pig macrophages have been analysed by chemical methods using nitrobenzene sulphonic acid surface labelled with /sup 125/I. The experiments provided strong evidence that the TNP specific immunogen which T lymphocytes recognise on guinea pig macrophages does not consist of directly haptenated Ia antigens.

  11. Erythropoietin and erythropoietin receptor expression in the guinea pig inner ear

    DEFF Research Database (Denmark)

    Cayé-Thomasen, Per; Wagner, Niels; Lidegaard Frederiksen, Birgitte

    2005-01-01

    , this study determines expression of EPO and EPOR in the inner ear of the guinea pig. Normal guinea pig inner ears were processed for immunohistochemistry, using poly-clonal antibodies against EPO and the EPO receptor. EPO expression was exclusively found in most, but not all spiral ganglion neurons...... expressed by several cell types within the guinea pig cochlea. We hypothesize on the existence of a local paracrine system and that EPO treatment may be feasible following inner ear damage....

  12. Estabelecimento de leguminosas arbóreas em pastos de capim-marandu e tanzânia Establishment of leguminous trees in marandu and tanzânia pastures

    Directory of Open Access Journals (Sweden)

    Paulo Francisco Dias

    2008-10-01

    Full Text Available O objetivo deste trabalho foi avaliar o desempenho de mudas sem proteção (de cercas ou estacas de quatro espécies de leguminosas arbóreas e uma mistura eqüitativa dessas espécies, introduzidas em pastagens de Brachiaria brizantha cv. Marandu e Panicum maximum cv. Tanzânia, na presença de gado. O delineamento experimental foi o inteiramente ao acaso, em esquema fatorial 2x5, duas gramíneas (marandu e tanzânia e quatro espécies de leguminosas (Mimosa artemisiana, Pseudosamanea guachapele, Enterolobium contortisiliquum, Acacia farnesiana e uma mistura dessas espécies, com três repetições. Avaliaram-se: altura da muda, diâmetro do caule, diâmetro da copa, sobrevivência da muda, freqüência de pastejo e ocorrência de formigas. As diferenças estatísticas entre as médias da variável canônica principal, pelo teste de Scott-Knott, indicaram a formação de três agrupamentos, tendo-se destacado o grupo formado pelos tratamentos M. artemisiana e mistura de leguminosas, nos dois pastos, mais E. contortisiliquum e A. farnesiana, nos pastos dos capins marandu e tanzânia, respectivamente. Diferenças entre as médias dos tratamentos relativas a cada variável, calculadas por meio de intervalos de confiança de Bonferroni, mostraram que mudas de M. artemisiana apresentaram maior altura e sobrevivência em pasto de capim-marandu. Mudas dessa leguminosa, sem proteção, são indicadas para ser introduzidas, nas pastagens de capim-marandu da região, na presença do gado.The objective of this work was to evaluate the performance of nonprotected (by fences or pickets seedlings of leguminous tree species and an equitable mix of these species, introduced in pastures of Brachiaria brizantha cv. Marandu and Panicum maximum cv. Tanzânia, in the presence of cattle. The experimental design was a completely randomized one, in a 2x5 factorial arrangement, with two grasses (marandu and tanzânia and four leguminous species (Mimosa artemisiana

  13. The preparation of recombinant interleukin-6 and its protection for irradiated guinea-pigs

    International Nuclear Information System (INIS)

    Wang Junfu; Zhang Jie; Zhang Jianhua; Feng Jinbo; Xu Jie; Wang Baohong

    2002-01-01

    Objective: To purify recombinant human interleukin-6 from gene-engineered E. coli and study the effects of IL-6 on survival period and platelet count of irradiated guinea-pigs. Methods: Recombinant human IL-6 was expressed in gene-engineered E. coli and purified with reversed phase chromatography. Purified IL-6 was then administered to irradiated guinea-pig. Results: IL-6 could significantly prolong the survival period and increase the platelet count of irradiated guinea-pigs. Conclusion: IL-6 showed its effective protection for irradiated guinea-pigs

  14. Men's behavior surrounding use of contraceptives in Guinea.

    Science.gov (United States)

    Baldé, Mamadou Diouldé; Diallo, Boubacar Alpha; Compaoré, Rachidatou; Bah, Abdoul Karim; Ali, Moazzam; Kabra, Rita; Kouanda, Seni

    2016-11-01

    To analyze the sociocultural determinants that influence the attitude and practices of men toward contraceptive use in Guinea. A sequential, mixed methods, qualitative and quantitative study was carried out in two regions of Guinea with a low rate of contraceptive prevalence, and in the capital city of Conakry. A total of 1170 people (men and women) were interviewed. Findings showed a positive perception of family planning overall, but reluctance to use modern contraception. The reasons for non-use of contraceptive methods were primarily the hope of having many children and religious prohibition associated with customs. Making decisions on contraceptive use within a couple represents a major cause of misunderstanding between spouses. Communication within a couple on the use of contraception is quickly declined by men. The findings demonstrate the need to develop communication strategies within a couple to improve the use of contraceptives. Copyright © 2016 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.

  15. An annotated checklist of the chondrichthyans of Papua New Guinea.

    Science.gov (United States)

    White, William T; Ko'ou, Alfred

    2018-04-19

    An annotated checklist of the chondrichthyan fishes (sharks, rays, and chimaeras) of Papua New Guinean waters is herein presented. The checklist is the result of a large biodiversity study on the chondrichthyan fauna of Papua New Guinea between 2013 and 2017. The chondrichthyan fauna of Papua New Guinea has historically been very poorly known due to a lack of baseline information and limited deepwater exploration. A total of 131 species, comprising 36 families and 68 genera, were recorded. The most speciose families are the Carcharhinidae with 29 species and the Dasyatidae with 23 species. Verified voucher material from various biological collections around the world are provided, with a total of 687 lots recorded comprising 574 whole specimens, 128 sets of jaws and 21 sawfish rostra. This represents the first detailed, verified checklist of chondrichthyans from Papua New Guinean waters.

  16. Aetiology of ''slobbers'' (chronic fluorosis) in the guinea-pig

    Energy Technology Data Exchange (ETDEWEB)

    Hard, G C; Atkinson, F F.V.

    1967-01-01

    A natural outbreak of ''slobbers'' with high morbidity and high mortality in guinea-pig breeding units in New South Wales and Canberra has been shown to be due to chronic fluoride poisoning. A survey of the dietary regiment employed in laboratories and units demonstrated the association of disease incidence with the feeding of proprietary pellets and usually low levels of green-food supplementation. Fluorine concentration in the pellets used in affected colonies ranged from 130 to 400 ppm. Inquiry produced some evidence that Christmas Island (Indian Ocean) rock-phosphate of high fluoride content had been incorporated in the pellets in place of bone flour. Experimental reproduction of the spontaneous disease was accomplished in exact detail by the extended administration of calcium fluoride in a concentration ranging from 300 to 450 ppm. The fluorine content of skull and teeth samples of experimentally poisoned guinea-pigs was comparable to that in natural cases of slobbers.

  17. Políticas agroambientales y ganadería en los montes de utilidad pública del País Vasco

    Directory of Open Access Journals (Sweden)

    Juan Cruz Alberdi Collantes

    2003-01-01

    Full Text Available Los terrenos y montes comunales han sido un recurso fundamental en la sociedad agraria vasca aportando toda una serie de aprovechamientos básicos para su funcionamiento como eran pastos, madera, helechales, agua, … Con su urbanización progresiva la explotación de muchos de sus recursos deja de tener sentido y estos espacios pasan a cumplir otras funciones, como áreas naturales, turísticas e incluso deportivas. Algunos aprovechamientos tradicionales perviven aunque han de adecuarse a las nuevas inquietudes sociales, como son las actividades ganaderas, que continúan cumpliendo una función básica en el mantenimiento del monte. Cambia su organización tradicional, antiguas prácticas de pastoreo son prohibidas y se recomiendan nuevas, los usos y áreas de aprovechamiento son delimitados. En definitiva, se incorporan conceptos medioambientales que contribuyen al correcto mantenimiento de estos espacios.

  18. del alcoholismo

    Directory of Open Access Journals (Sweden)

    Rodrigo Arias Duque

    2005-01-01

    Full Text Available Desde el punto de vista farmacológico, es importante comprender qué es el alcohol y cómo actúa en el organismo. No existe una causa simple, sino una interacción complicada de factores neuroquímicos, fisiológicos, psicológicos y sociales que originan y desarrollan esta grave enfermedad fármaco-dependiente. La acción psicofisiológica y farmacodinámica del alcohol es fundamentalmente depresiva, por la reducción de la transmisión sináptica en el sistema nervioso humano. Es un hecho conocido que el consumo excesivo de alcohol causa una disfunción aguda y crónica del cerebro, produciendo trastornos en el sistema nervioso central, presentando alteraciones en la memoria y en las funciones intelectuales como cálculo, comprensión y aprendizaje. A nivel hepático tiene lugar, en su mayoría, el metabolismo del alcohol, produciéndose un hígado graso alcohólico, aumentando el tamaño, terminando en necrosis e inflamación grave del hígado; esto se llama hepatitis alcohólica, y si se sigue consumiendo alcohol se desarrollará la cirrosis. El alcohol también ha estado relacionado con alteraciones del miocardio; se ha constatado en animales de experimentación sanos que tanto la velocidad de contracción del músculo cardiaco y su máxima tensión disminuyen en presencia del alcohol, como consecuencia, la fuerza de cada contracción y el aumento de presión en el ventrículo izquierdo son menores, perdiendo eficacia el corazón como bomba.

  19. Pharmacology of Bradykinin-Evoked Coughing in Guinea Pigs

    OpenAIRE

    Hewitt, Matthew M.; Adams, Gregory; Mazzone, Stuart B.; Mori, Nanako; Yu, Li; Canning, Brendan J.

    2016-01-01

    Bradykinin has been implicated as a mediator of the acute pathophysiological and inflammatory consequences of respiratory tract infections and in exacerbations of chronic diseases such as asthma. Bradykinin may also be a trigger for the coughing associated with these and other conditions. We have thus set out to evaluate the pharmacology of bradykinin-evoked coughing in guinea pigs. When inhaled, bradykinin induced paroxysmal coughing that was abolished by the bradykinin B2 receptor antagonis...

  20. Dermal Sensitization Potential of Diethyleneglycol Dinitrate (DEGDN) in Guinea Pigs

    Science.gov (United States)

    1988-10-01

    Woodard G, Calvery HO. Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membxanes. J Pharmacol...the attached data sheet, ARFPOM Forn 213R. The compound chromatographed as a single peak ( retention time 5.4 r!’n) by HPLC analysis under the...commonly encountered in guinea pigs. 3. The right eye of animal 85E0132 had a congenital dermoid cyst in the conjunctiva. The liver of animal 85E0112

  1. Management and utilization of forest resources in Papua New Guinea

    Science.gov (United States)

    P.B.L. Srivastava

    1992-01-01

    Papua New Guinea, with an area of about 46.7 million ha and population of 3.7 million, is blessed with a large natural forest resource. Over 80 percent of the land is covered with forests of various types, ranging from swamp and lowland rain forests in coastal plains to alpine vegetation and moss forests in the highlands, most of which are owned by the people. About 15...

  2. Carriers, channels and chloroquine efficacy in Guinea-Bissau

    DEFF Research Database (Denmark)

    Ursing, Johan; Rombo, Lars; Kofoed, Poul-Erik

    2008-01-01

    Recent studies suggest that chloroquine resistance is mediated by an energy-dependent saturable chloroquine efflux carrier. An alternative explanation is that resistance is mediated by a channel. In Guinea-Bissau high doses of chloroquine are effective, well-tolerated and commonly used....... This suggests that chloroquine resistance can be overcome by higher doses. Research on the mechanism of chloroquine resistance is of utmost importance and should include the effect of higher doses....

  3. Transfer of radiobismuth to the fetus in guinea-pigs

    International Nuclear Information System (INIS)

    Richardson, R.B.

    1992-01-01

    A small animal study was carried out in guinea-pigs to measure uptake of radiobismuth ( 207 BiCl 3 ) in fetal organs after intravenous administration to the mother. In this study, little activity was found to cross the placental barrier with 214 Bi across the placenta, is considered to result in a negligible dose from radon short-lived daughters to fetal organs. (author)

  4. Beam-Beam Simulations with GUINEA-PIG

    CERN Document Server

    Schulte, Daniel

    1998-01-01

    While the bunches in a linear collider cross only once, due to their small size they experience a strong beam-beam effect. GUINEA-PIG is a code to simulate the impact of this effect on luminosity and back ground. A short overview of the program is given with examples of its application to the back ground strudies for TESLA, the top quark threshold scan and a possible luminosity monitor, as well as some results for CLIC.

  5. The history of postcolonial kinglets in Guinea Bissau

    Directory of Open Access Journals (Sweden)

    Clara Carvalho

    2009-10-01

    Full Text Available This paper attempts to demonstrate that the role of traditional authorities in today’s Guinea Bissau is embedded in a long-term history that goes back to their pre-colonial and colonial history. Looking back to the situation of traditional authorities by the end of the colonial period (the early ‘70s, the paper discusses the different configurations of legitimacy of present-day authorities in relation to both local population and State authorities.

  6. Guinea: Background and Relations with the United States

    Science.gov (United States)

    2010-07-19

    multinational aluminum firm Alcoa, for instance, is a major shareholder in the Compagnie des Bauxites de Guinee, a bauxite mining and export partnership...world’s largest bauxite reserves, and joint-venture bauxite mining and alumina operations have historically provided about 80% of Guinea’s foreign...announced the approval of a $200 million loan to partly finance a $6.3 billion bauxite mining and alumina refinery project in Guinea. The project was

  7. Charting the neglected West: The social system of Guinea baboons.

    Science.gov (United States)

    Fischer, Julia; Kopp, Gisela H; Dal Pesco, Federica; Goffe, Adeelia; Hammerschmidt, Kurt; Kalbitzer, Urs; Klapproth, Matthias; Maciej, Peter; Ndao, Ibrahima; Patzelt, Annika; Zinner, Dietmar

    2017-01-01

    Primate social systems are remarkably diverse, and thus play a central role in understanding social evolution, including the biological origin of human societies. Although baboons have been prominently featured in this context, historically little was known about the westernmost member of the genus, the Guinea baboon (Papio papio). Here, we summarize the findings from the first years of observations at the field site CRP Simenti in the Niokolo Koba National Park in Senegal. Guinea baboons reveal a nested multi-level social organization, with reproductive units comprising one "primary" male, one to several females, young, and occasionally "secondary" males at the base of the society. Three to five units form "parties," which team up with other parties to form a "gang." Different gangs have largely overlapping home ranges and agonistic interactions between different parties or gangs are rare. Some but not all strongly socially bonded males are highly related, and population genetic and behavioral evidence indicate female-biased dispersal. Females play an important role in intersexual bond formation and maintenance, and female tenure length varies between a few weeks to several years. While the social organization resembles that of hamadryas baboons (P. hamadryas), the social structure differs considerably, specifically in terms of low male aggressiveness and female freedom. Despite substantial differences in social organization and social structure, the acoustic structure of Guinea baboon vocalizations does not differ substantially from that of other baboon taxa. With its multi-level organization, stable bonds between males and females, as well as a high-degree of male-male cooperation and tolerance, Guinea baboons constitute an intriguing model for reconstructing human social evolution. © 2017 American Association of Physical Anthropologists.

  8. Child health and mortality in Guinea-Bissau

    DEFF Research Database (Denmark)

    Kovsted, Jens Anders; Pörtner, Claus Christian; Tarp, Finn

    2002-01-01

    This paper studies factors that influence child health in Bissau, the capital of Guinea-Bissau. This environment is characterised by high infant mortality, but not by malnutrition. We show that although maternal education is important in determining child health and mortality this effect diminishes...... or disappears when health knowledge is introduced as an explanatory variable. It emerges that health knowledge has large and positive effects on both child mortality and health when instrumented for to capture endogeneity...

  9. Diversidad zoológica asociada a un silvopastoreo leucaena-guinea con diferentes edades de establecimiento Zoological diversity associated to a silvopastural system leucaena-guinea grass with different establishment times

    Directory of Open Access Journals (Sweden)

    Jatnel Alonso Lazo

    2007-12-01

    Full Text Available El objetivo de este trabajo fue evaluar la diversidad zoológica asociada a un silvopastoreo con leucaena-guinea, por medio de la caracterización de la composición y estructura de las aves, insectos y la macrofauna del suelo, en cuatro edades de establecimiento (3, 4, 5 y 6 años de explotación. Con las especies registradas en cada uno de estos grupos zoológicos, se calcularon los índices ecológicos: número de individuos, riqueza, diversidad y abundancia de especies, en diferentes edades del sistema. En todos los grupos, se apreció el aumento significativo en la riqueza de especies y en el índice de diversidad biológica de Shannon, en la medida que se desarrolló el sistema. Se observó incremento en la abundancia de insectos biorreguladores y, en relación con las aves, el horario de muestreo no mostró interacción con los distintos años de siembra. La macrofauna se incrementó, observándose dominancia de anélidos al 6º y 7º año de explotación, caracterizado por Polyferetrina elongata y Oligochaeta elegans. El desarrollo del silvopastoreo leucaena-guinea logra sistemas productivos pecuarios que aumentan la producción de biomasa y de otros componentes biológicos y contribuir para crear un sistema sostenible y compatible con el ambiente.The aim of this work was to evaluate the associated zoological diversity of a silvopastural system leucaena-guinea grass, by characterizing the composition and structures of the birds, insects and the macrofauna of the soil, in four establishment times of the silvopastural systems (3, 4, 5 and 6 years of exploitation. For the species recorded in each zoological group, the following ecological indices were determined: number of individuals, richness, diversity and abundance of species, in each establishment times of the system. A significant increase, in all the zoological groups, was observed for the richness of species and for the index of biological diversity of Shannon, as the system

  10. Studies of guinea pig immunoglobulin isotype, idiotype and antiidiotype

    International Nuclear Information System (INIS)

    Tirrell, S.M.

    1988-01-01

    Immunization of Guinea pigs with diphtheria toxoid generated antibodies of the IgG class that were capable of neutralizing native toxin in vivo. Sera from these animals were used to affinity purify idiotypic antibodies (AB1). AB1 vaccines derived from the IgG1 class and from F(ab') 2 of IgG1 + IgG2 (IgG1/2) classes were effective in inducing a syngeneic anti-idiotype (AB2) response. Animals immunized with AB1 consisting of both IgG1/2 did not elicit a detectable AB2 response. Binding of homologous 125 I-F(ab') 2 (AB1) to the antiidiotype was inhibited 90% in the presence of DT.F(ab') 2 derived from preimmune serum or had no inhibitory effects on the idiotype-antiidiotype interactions. Two groups of outbred guinea pigs were vaccinated with alum absorbed F(ab') 2 of anti-idiotype IgG1/2 (AB2). Of the ten animals inoculated with AB2, three tested positive by RIA against 125 I-DT. Two of the RIA positive sera contained antibodies that neutralized diphtheria toxin in a rabbit intracutaneous assay. Purification of guinea pig IgG by protein A-Sepharose affinity chromatography resulted in the separation of three distinct IgG populations

  11. Guinea pigs as an animal model for sciatic nerve injury

    Directory of Open Access Journals (Sweden)

    Malik Abu Rafee

    2017-01-01

    Full Text Available The overwhelming use of rat models in nerve regeneration studies is likely to induce skewness in treatment outcomes. To address the problem, this study was conducted in 8 adult guinea pigs of either sex to investigate the suitability of guinea pig as an alternative model for nerve regeneration studies. A crush injury was inflicted to the sciatic nerve of the left limb, which led to significant decrease in the pain perception and neurorecovery up to the 4th weak. Lengthening of foot print and shortening of toe spread were observed in the paw after nerve injury. A 3.49 ± 0.35 fold increase in expression of neuropilin 1 (NRP1 gene and 2.09 ± 0.51 fold increase in neuropilin 2 (NRP2 gene were recorded 1 week after nerve injury as compared to the normal nerve. Ratios of gastrocnemius muscle weight and volume of the experimental limb to control limb showed more than 50% decrease on the 30th day. Histopathologically, vacuolated appearance of the nerve was observed with presence of degenerated myelin debris in digestion chambers. Gastrocnemius muscle also showed degenerative changes. Scanning electron microscopy revealed loose and rough arrangement of connective tissue fibrils and presence of large spherical globules in crushed sciatic nerve. The findings suggest that guinea pigs could be used as an alternative animal model for nerve regeneration studies and might be preferred over rats due to their cooperative nature while recording different parameters.

  12. Vestibular morphology in the German Waltzing guinea pig.

    Science.gov (United States)

    Kawaguchi, Sachie; Hultcrantz, Malou; Jin, Zhe; Ulfendahl, Mats; Suzuki, Mamoru

    2010-04-01

    The German waltzing guinea pig is a special strain of animal with a recessively inherited inner ear defect, resulting in deafness and a severe vestibular dysfunction. The hearing loss in the cochlea of the German strain is a result of a collapse of the Reissner membrane and the absence of scala media. The vestibular organ has not yet been described. German waltzing guinea pigs (homozygote and heterozygote) of different ages ranging from embryologic age 25 days to adulthood were investigated. The living animals were tested with four different vestibular tests, and the fetuses were controlled according to breeding. The morphology of the vestibular parts (ampulla, saccule, and utricle) was observed by using the light and transmission electron microscopy. Collapse of the membranous labyrinth was found already at embryologic age 50 days and progressed over time. Vestibular dysfunction was noted already from birth. Vestibular atelectasis has been shown to have the same morphology as the reported vestibular dysfunction in the German waltzing guinea pig. Owing to this similarity, this animal can be a good model for vestibular research.

  13. Genetic factors of Ebola virus virulence in guinea pigs.

    Science.gov (United States)

    Subbotina, Ekaterina; Dadaeva, Alexandra; Kachko, Alla; Chepurnov, Alexander

    2010-10-01

    Zaire ebolavirus (ZEBOV) causes severe hemorrhagic fever in primates, whereas in guinea pigs it induces a nonlethal infection with a mild fever and subsequent recovery. We performed 7 selective passages in guinea pigs resulted in obtaining of guinea pig-adapted strain (GPA-P7) strain. By the 7th passage, the infection with EBOV induced a lethal disease in animals accompanied by the characteristic hematological changes: leukocytosis (primarily due to neutrophilia) as well as pronounced deficiencies in platelets, lymphocytes, monocytes and significant decrease of blood neutrophils phagocytic capacity. Increasing of virulence correlated with appearance of several nucleotide substitutions: in the genes NP, A2166G (N566S), VP24, U10784C (L147P), G10557A (M71I), G10805U (R154L), and L, G12286A (V236I). It has been theoretically calculated that the mutations associated with an increase in EBOV virulence can confer characteristic secondary structure on the proteins NP (C-terminal region) and full-sized VP24. (c) 2010 Elsevier B.V. All rights reserved.

  14. UTILIDAD DE LAS INVESTIGACIONES MONOGRÁFICAS EN LA URACCAN, RECINTO NUEVA GUINEA

    Directory of Open Access Journals (Sweden)

    Oved Zeledón Membreño

    2012-10-01

    Full Text Available La investigación permitió conocer el estado actual y utilidad de las  monografías existentes en la biblioteca “Luz en la Selva” de la  URACCAN, recinto Nueva Guinea, 2003 – 2009.  Fue un estudio mixto, con predominancia del enfoque cualitativo, se utilizaron técnicas de recolección de datos como revisión bibliográfica, encuestas y entrevistas a través de las cuales se obtuvo resultados acerca de la clasificación de las 115 monografías realizadas entre los años 2003-2009, se destaca con mayor aporte las áreas de Ciencias de la Educación, Humanidades y Recursos Naturales y Medio Ambiente, porque exige entre sus requisitos de culminación los trabajos monográficos. El enfoque fue mixto con predominancia cualitativa. Las principales líneas de investigación fueron las de desarrollo socioeconómico ambiental y metodologías de enseñanza-aprendizaje. La comunidad universitaria tiene conocimiento de la existencia de las monografías en la biblioteca; sin embargo, las formas de divulgación no han sido efectivas. Las monografías han presentado diferentes utilidades: generando proyectos, fuente de otras investigaciones y resolución de problemáticas comunitarias.

  15. ALTERNATIVA DE CORREDOR ECOLÓGICO EN LA ZONA NORORIENTAL DEL MUNICIPIO DE MEDELLÍN

    Directory of Open Access Journals (Sweden)

    VILLA GRAJALES ANA MARÍA

    2012-11-01

    Full Text Available El presente trabajo se realizó con el objetivo de establecer una alternativa de corredor ecológico de conexión estructural en las comunas 1, 2, 3 y 4 de la zona nor-oriental del municipio de Medellín, orientada a la  protección del suelo verde existente y a la toma de decisiones en cuanto a la generación de nuevo suelo, utilizando las métricas del paisaje y herramientas geoinformaticas. Se utilizó un área de 400 m² como Unidad Mínima Cartografiable (UMC, encontrándose 296 ha (19,4% de áreas verdes respecto a las 1.527 ha de área de estudio, predominando la cobertura de pasto con 95,4 ha, mientras que la zona arbolada, con un área de 26,1 ha, mostró el mayor aislamiento (99,1 m, y el rastrojo alto con 48,7 ha presentó la mayor agregación (16,4 m. Se priorizaron ocho (8 fragmentos para la conservación, seleccionados a través del Índice de Calidad del Fragmento (ICF, sumando en total 26 ha. El corredor se estableció con un ancho de 80 m, arrojando un área total de 73,2 ha, asociada, en su mayoría, a retiros de quebradas.

  16. Interstitial cells of Cajal and Auerbach's plexus. A scanning electron microscopical study of guinea-pig small intestine

    DEFF Research Database (Denmark)

    Jessen, Harry; Thuneberg, Lars

    1991-01-01

    Anatomy, interstitial cells of Cajal, myenteric plexus, small intestine, guinea-pig, scanning electron microscopy......Anatomy, interstitial cells of Cajal, myenteric plexus, small intestine, guinea-pig, scanning electron microscopy...

  17. Optimization of Glial Fibrillary Acidic Protein Immunoreactivity in Formalin-fixed, Paraffin-Embedded Guinea Pig Brain Sections

    Science.gov (United States)

    2003-09-01

    fixed, paraffin-embedded guinea pig brain sections using a variety of commercially available GFAP antibody clones. Of the 7 clones tested for cross...determining neuropathological consequences in the guinea pig following exposure to chemical warfare nerve agent.

  18. Características morfogênicas e estruturais de perfilhos de capim-braquiária em locais do pasto com alturas variáveis Morphogenic and structural characteristics of tillers on areas with signalgrass pasture varying on height

    Directory of Open Access Journals (Sweden)

    Manoel Eduardo Rozalino Santos

    2011-03-01

    Full Text Available O experimento foi conduzido com o objetivo de avaliar a influência das variações naturais da altura da planta no mesmo pasto sobre as características morfogênicas e estruturais da Brachiaria decumbens cv. Basilisk manejada sob lotação contínua com bovinos. Foram avaliadas quatro alturas de plantas (10, 20, 30 e 40 cm no mesmo pasto manejado com altura média de 25 cm. Adotou-se o delineamento em blocos ao acaso com duas repetições. A taxa de aparecimento foliar do capim-braquiária foi influenciada linear e negativamente pelas alturas das plantas no mesmo pasto. Os perfilhos presentes nos locais com maior altura apresentaram maior filocrono (9,3 dias. A maior altura das plantas no mesmo pasto promoveu maiores taxas de senescência foliar e de alongamento de pseudocolmo no perfilho. A duração de vida da folha (37,8 dias e a taxa de alongamento foliar (1,3 cm/perfilho.dia não foram influenciadas pela altura da planta. Os números de folhas vivas (4,36 e mortas (2,15 também não foram modificados pela altura da planta. Já a maior altura das plantas resultou em aumento linear de 69% no número de folhas com desfolhação. Os comprimentos do pseudocolmo e da lâmina foliar responderam linear e positivamente ao aumento na altura da planta no mesmo pasto. A variação natural na altura da planta no mesmo pasto altera as características morfogênicas e estruturais do capim-braquiária. Existe variabilidade espacial da vegetação em pastos de Brachiaria decumbens cv. Basilisk manejados sob lotação contínua com bovinos.The objective of this experiment was to evaluate the influence of natural variation of heights of plants on the same pasture on the morphogenic and structural characteristics of Brachiaria decumbens cv. Basilisk managed under continuous stocking with cattle. Four plant heights (10, 20, 30 and 40 cm were evaluated on the same pasture managed at an average height of 25 cm. It was used a randomized block design with two

  19. Asociación de cepas fijadoras de nitrógeno de vida libre, con recursos genéticos de pastos para zonas áridas.

    OpenAIRE

    Plascencia Jiménez, Raúl

    2014-01-01

    Para evaluar microorganismos de la rizosfera de Bouteloua curtipendula (Michx.) Torr., se recolectó suelo de cinco localidades 1) Salinas, San Luis Potosí 2) Ejido, Jacalón, Salinas, San Luis Potosí; 3) Calera, Zacatecas; 4) Santa María del Oro, Durango y 5) Crucero La Zarca, Durango. La población de actinomicetos, bacterias y hongos, se estimó con diluciones seriadas (10-1 a 10-10). Los actinomicetos (AS), bacterias (BS) y hongos (HS) se estimaron con diluciones 10-3 a 10-5; 10-5 a 10-7; 10-...

  20. "Navajita Cecilia" Bouteloua gracilis H.B.K (Lag.. Nueva variedad de pasto para zonas áridas y semiáridas

    Directory of Open Access Journals (Sweden)

    Sergio Beltrán López

    2010-01-01

    fertilización nitrogenada. Los resultados muestran que el uso combinado de inoculante más 30 kg N ha-1 produjo 50 % más MS (aproximadamente 11 t más que cuando se aplica sólo nitrógeno, especialmente en avena y ballico anual. El análisis económico mostró que el mejor tratamiento fue el de inoculación con 30 kg N ha-1 en Avena sativa L, con una tasa de retorno marginal por arriba del 60 %.

  1. Culturas del Mundo

    DEFF Research Database (Denmark)

    Benwell, Ann Fenger; Costa, Alberto; Waehle, Espen

    2006-01-01

    ’Culturas del mundo. Colecciones del Museo Nacional de Dinamarca’ with Ann Fenger Benwell in Culturas del Mundo. Colecciones del Museo Nacional de Dinamarca, ed. Silvia Sauquet, Fundación "la Caixa", Barcelona 2006, pp. 31-39......’Culturas del mundo. Colecciones del Museo Nacional de Dinamarca’ with Ann Fenger Benwell in Culturas del Mundo. Colecciones del Museo Nacional de Dinamarca, ed. Silvia Sauquet, Fundación "la Caixa", Barcelona 2006, pp. 31-39...

  2. EXPOSICIÓN AL MONÓXIDO DE CARBONO DEL PERSONAL ESPECIALISTA EN EXTINCIÓN DE INCENDIOS FORESTALES

    Directory of Open Access Journals (Sweden)

    Belén Carballo Leyenda

    2010-01-01

    Full Text Available Fundamento: Se ha descrito que la salud y el rendimiento laboral del personal dedicado a la extinción de los incendios forestales se ven perjudicados principalmente por el monóxido de carbono (CO. El objetivo de este trabajo ha sido analizar la exposición al CO a la que se ve sometido el personal especialista en la extinción de los incendios. Métodos: Durante 58 incendios reales se monitorizó en 44 sujetos la exposición al CO a la que se vieron sometidos. También se analizó la concentración ponderada para una jornada de 8h (VA-ED. Todos los incendios fueron divididos en función del tipo de trabajo realizado (ataque directo, indirecto y combinado y del combustible presente (pasto, matorral, bajo arbolado y mixto. Las variables analizadas fueron estudiadas mediante la prueba de Kruskal-Wallis, se usó el test de Mann-Whitney para establecer las diferencias entre medias. Resultados: La concentración media de CO en los incendios fue de 18,4±1,7 ppm, lo que supuso una VA-ED de 7,0±1,0 ppm. Las mayores exposiciones al CO se analizaron en los ataques combinados (20,4±2,3 ppm seguidas de las obtenidas en los ataques directos (17,5±2,7 ppm e indirectos (10,6±5,4 ppm. Únicamente se obtuvieron diferencias significativas (p<0,05 al comparar las exposiciones al CO generadas por la combustión del matorral (19,8±2,2 y bajo arbolado (17,2±3,9 y pasto (12,0±5,6. Conclusiones: Las exposiciones de CO analizadas se vieron influidas por el tipo de combustible y trabajo desempeñado. Los valores medios obtenidos se situaron dentro de los límites establecidos como seguros por diferentes organismos nacionales (INSHT e internacionales (NIOSH, OSHA.

  3. The Anticomplementary Activity of ’Fusobacterium polymorphum’ in Normal and C-4 Deficient Sources of Guinea Pig Complement.

    Science.gov (United States)

    1977-01-12

    A complement consumption assay was used to show that the anticomplementary activity of a cell wall preparation from F. polymorphum in guinea pig complement...tests with C𔃾-deficient guinea pig sera confirmed that F. polymorphum cell walls were capable of generating alternate complement pathway activity in guinea pig sera.

  4. Verifica della qualitá delle indagini epidemiologiche per eventi epidemici di tossinfezione alimentare: l'esperienza del Servizio Igiene Pubblica della ASL RMG

    Directory of Open Access Journals (Sweden)

    A. Lancia

    2003-05-01

    Full Text Available

    Obiettivi: obiettivo dello studio è verificare il miglioramento della qualità delle indagini epidemiologiche per eventi epidemici di tossinfezione alimentare dopo l’implementazione di un protocollo aziendale condiviso tra i servizi del Dipartimento di Prevenzione della ASL RMG (SIP-SIAN-SV e l’attivazione della pronta disponibilità di una equipe dedicata.

    Metodi: sono state presi in considerazione tutti gli eventi epidemici di tossinfezione alimentare individuati dal SIP nel periodo 1998-2002: il periodo A (1998-1sem2000 è quello precedente all’attivazione del protocollo e il periodo B (2sem 2000-2002 è quello successivo. È stato realizzato un questionario per la raccolta retrospettiva dei dati archiviati; l’informatizzazione è stata effettuata con il programma EPIinfo vers. 6.0 e l’analisi con il software microsoft excel vers 7.0. Risultati: nel periodo A sono stati individuati n.14 eventi epidemici per un totale di 76 malati. Il servizio SIAN e/o SV è stato attivato nel 28% dei casi, il sopralluogo nel luogo di consumo del pasto è stato effettuato nel 42%(6/14 e nel 33% dei casi il campionamento dell’alimento ha individuato l’agente microbiologico. Nel periodo B invece sono stati individuati n.21 eventi epidemici per un totale di 202 malati. Il servizio SIAN e/o SV è stato attivato nel 71% dei casi, il sopralluogo nel luogo di consumo del pasto è stato effettuato nel 57%(12/21 e nel 57% dei casi il campionamento dell’alimento ha individuato l’agente microbiologico.

    Conclusioni: in seguito all’attivazione del protocollo è migliorata la qualità dei dati raccolti. L’istituzione di una equipe dedicata ha determinato una più accurata sorveglianza degli episodi di tossinfezione attraverso una maggiore efficienza nell’attivazione dei servizi SIAN-SV. Questa esperienza dimostra che l’integrazione tra i Servizi del Dipartimento di Prevenzione

  5. On a new bird of paradise from Central New Guinea, Falcinellus meyeri albicans

    NARCIS (Netherlands)

    Oort, van E.D.

    1915-01-01

    Among the collection of birdskins brought home by the third expedition to Mount Wilhelmina in the Snow Mountains of New Guinea there are some specimens, males and females, of a bird of paradise, much resembling Falcinellus meyeri (Finsch) from South-east New Guinea, but differing in some points.

  6. Review: Joschka Philipps, Ambivalent Rage: Youth Gangs and Urban Protest in Conakry, Guinea (2013)

    OpenAIRE

    Ineke van Kessel; Afrika-Studiecentrum Leiden

    2014-01-01

    Review of the Monograph:Joschka Philipps, Ambivalent Rage: Youth Gangs and Urban Protest in Conakry, Guinea, Paris: L’Harmattan, 2013, ISBN 978-2-343-01577-4, 238 pp. Besprechung der Monographie:Joschka Philipps, Ambivalent Rage: Youth Gangs and Urban Protest in Conakry, Guinea, Paris: L’Harmattan, 2013, ISBN 978-2-343-01577-4, 238 Seiten

  7. The innate immunity of guinea pigs against highly pathogenic avian influenza virus infection.

    Science.gov (United States)

    Zhang, Kun; Xu, Wei Wei; Zhang, Zhaowei; Liu, Jing; Li, Jing; Sun, Lijuan; Sun, Weiyang; Jiao, Peirong; Sang, Xiaoyu; Ren, Zhiguang; Yu, Zhijun; Li, Yuanguo; Feng, Na; Wang, Tiecheng; Wang, Hualei; Yang, Songtao; Zhao, Yongkun; Zhang, Xuemei; Wilker, Peter R; Liu, WenJun; Liao, Ming; Chen, Hualan; Gao, Yuwei; Xia, Xianzhu

    2017-05-02

    H5N1 avian influenza viruses are a major pandemic concern. In contrast to the highly virulent phenotype of H5N1 in humans and many animal models, guinea pigs do not typically display signs of severe disease in response to H5N1 virus infection. Here, proteomic and transcriptional profiling were applied to identify host factors that account for the observed attenuation of A/Tiger/Harbin/01/2002 (H5N1) virulence in guinea pigs. RIG-I and numerous interferon stimulated genes were among host proteins with altered expression in guinea pig lungs during H5N1 infection. Overexpression of RIG-I or the RIG-I adaptor protein MAVS in guinea pig cell lines inhibited H5N1 replication. Endogenous GBP-1 expression was required for RIG-I mediated inhibition of viral replication upstream of the activity of MAVS. Furthermore, we show that guinea pig complement is involved in viral clearance, the regulation of inflammation, and cellular apoptosis during influenza virus infection of guinea pigs. This work uncovers features of the guinea pig innate immune response to influenza that may render guinea pigs resistant to highly pathogenic influenza viruses.

  8. The guinea pig maximization test--with a multiple dose design

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Vølund, A; Frankild, S

    1995-01-01

    The guinea pig maximization test (GPMT) is usually performed with one moderately irritant induction dose of the allergen and gives a qualitative assessment-hazard identification-of the allergenicity of the chemical. We refined the GPMT by applying a multiple dose design and used 30 guinea pigs in...

  9. Location of bladder and urethral sphincter motoneurons in the male guinea pig (Cavia porcellus)

    NARCIS (Netherlands)

    Kuipers, R; Izhar, Z; Gerrits, PO; Miner, W; Holstege, G; Gerrits, Peter O.

    2004-01-01

    Although the guinea pig is used widely in experimental medical research, including in studies on micturition control, the spinal origin of preganglionic parasympathetic bladder and somatic external urethral sphincter motoneurons is not known. In the male, guinea pig using wheat germ

  10. Review: Mohamed Saliou Camara, Political History of Guinea since World War Two (2014)

    OpenAIRE

    Carole Ammann; University of Basel

    2015-01-01

    Review of the monograph:Mohamed Saliou Camara, Political History of Guinea since World War Two, New York: Peter Lang, 2014, ISBN 9781433122439, 531 pp. Besprechung der Monographie:Mohamed Saliou Camara, Political History of Guinea since World War Two, New York: Peter Lang, 2014, ISBN 9781433122439, 531 Seiten

  11. Caridean shrimps (Crustacea, Decapoda) from seagrass habitats in Hansa Bay, Papua New Guinea

    NARCIS (Netherlands)

    Grave, de Sammy

    1999-01-01

    Five species of caridean shrimps collected from seagrass habitats at Hansa Bay, on the northern coastline of Papua New Guinea are reported. Four species are new to the fauna of Papua New Guinea. Morphological details of Nikoides danae, Latreutes pymoeus and L. porcinus are discussed and compared to

  12. First record of Mugil capurrii (Mugilidae, Perciformes) in the Gulf of Guinea.

    Science.gov (United States)

    Trape, S; Durand, J-D

    2011-03-01

    Leaping African mullet Mugil capurrii was caught along the Togolese coast in the Gulf of Guinea. This is the first record of this species which usually occurs from Morocco to Guinea Bissau and the southernmost point of its known distribution. © 2011 The Authors. Journal of Fish Biology © 2011 The Fisheries Society of the British Isles.

  13. The genus Baeturia Stål as represented in New Guinea (Homoptera, Cicadidae)

    NARCIS (Netherlands)

    Blöte, H.C.

    1960-01-01

    Until now only three species of the genus Baeturia have been recorded from New Guinea, viz., B. bicolorata Distant, B. viridicata Distant, and B. nana Jacobi. As I had the opportunity to study a fairly large collection of this group from different sources, collected in New Guinea, it appeared to me

  14. Interrelationships of Non-Formal Mother Tongue Education and Citizenship in Guinea and Senegal

    Science.gov (United States)

    Clemons, Andrea; Yerende, Eva

    2009-01-01

    Guinea and Senegal are multilingual countries that use French as a language of instruction in the formal educational sector with some significant exceptions. As in many other African countries, such exceptions in Guinea and Senegal, use local African languages primarily in the non-formal sector for a variety of purposes, such as adult literacy and…

  15. Mosses of d’Entrecasteaux group & Louisiade Archipelago, Papua, New Guinea

    NARCIS (Netherlands)

    Bartram, Edwin B.

    1960-01-01

    The moss collections made by L. J. Brass in connection with the 5th, Archbold Expedition to New Guinea to New Guinea in 1956 to the islands off the eastern tip of Papua comprise 166 numbers representing 87 species as outlined in the following list. Three species are described as new and 22 preceeded

  16. Epizootic guinea pig herpes-like virus infection in a breeding colony.

    Science.gov (United States)

    Connelly, B L; Keller, G L; Myers, M G

    1987-01-01

    A breeding colony of strain-2 guinea pigs which had been relatively free of indigenous caviid herpesviruses experienced an explosive outbreak of guinea pig herpes-like virus apparently as a consequence of intermixing groups and contamination of the water supply. A new breeding colony has been established and has been maintained apparently free of recognized caviid herpesviruses.

  17. Behavioral responses of deafened guinea pigs to intracochlear electrical stimulation: a new rapid psychophysical procedure

    NARCIS (Netherlands)

    Agterberg, M.J.H.; Versnel, H.

    2014-01-01

    In auditory research the guinea pig is often preferred above rats and mice because of the easily accessible cochlea and because the frequency range of its hearing is more comparable to that of humans. Studies of the guinea-pig auditory system primarily apply histological and electrophysiological

  18. [Mitochondrial DNA4568 deletions in guinea-pig associated with presbycusis].

    Science.gov (United States)

    Wei, Xue-mei; Yang, Yuan; Liang, Chuang-yu; Zheng, Zhong

    2006-12-01

    To determine weather or not the mtDNA(4568) deletions in guinea-pig contribute to the development of presbycusis. Forty-four guinea-pigs were divided into 2 groups: group A (young control group, normal hearing, 22 guineas) and group B (aged group). The group B was subdivided into group B(1) (old normal hearing, 6 guineas) and group B(2) (old hearing loss, 16 guineas). First the guineas were tested by auditory brainstem response (ABR), and then the Cortis's tissues, auditory nerve tissues, brain and blood were harvested and the total DNA was extracted. The mtDNA(4568) deletion was analyzed by PCR. Hearing loss was occurred with age. The mtDNA(4568) deletion incidence of aged group in all tissues was significant higher than that of young control group (Ppresbycusis (B(2) group) were significant higher than that of aged normal hearing group (B(1) group) (Ppresbycusis and aged normal hearing group (P> 0.05). mtDNA(4568) deletion of guinea-pig possibly contributes to aging and mtDNA(4568) deletion in Cortis's and auditory nerve tissues of guinea-pig may be associated with presbycusis. There is no enough evidence to prove that the mtDNA(4568) deletions in brain and blood are related with presbycusis.

  19. Anticonvulsant Effects of Memantine and MK-801 in Guinea Pig Hippocampal Neurons.

    Science.gov (United States)

    investigation we compared the anticonvulsant properties of Mem to those of MK-801 in guinea pig hippocampal slices. Extracellular recordings were...obtained from area CA1 of guinea pig hippocampal slices in a total submersion chamber at 32 deg C in normal oxygenated artificial cerebrospinal fluid (ACSF

  20. Evaluating of the Anticonvulsant Gabapentin against Nerve Agent-Induced Seizures in a Guinea Pig Model

    Science.gov (United States)

    2010-07-01

    treating neuropathic pain. This study evaluated whether gabapentin could terminate or moderate nerve agent-induced seizures using a validated guinea ... pig model. Male Hartley guinea pigs were surgically prepared to record electroencephalographic (EEG) activity. After a week recovery, animals were

  1. Nickel-sulphate-induced contact dermatitis in the guinea pig maximization test

    DEFF Research Database (Denmark)

    Rohold, A E; Nielsen, G D; Andersen, Klaus Ejner

    1991-01-01

    Nickel sulphate is a sensitizer in guinea pigs, but the frequency of sensitization varies from study to study. The dose-response relationship for NiSO4.6H2O was evaluated in the guinea pig maximization test in this study. 6 intradermal (0.01%-3.0% aq.) and 6 topical (0.25%-10.0% pet.) concentrati...

  2. Molecular cloning, expression, and in silico structural analysis of guinea pig IL-17.

    Science.gov (United States)

    Dirisala, Vijaya R; Jeevan, Amminikutty; Ramasamy, Suresh K; McMurray, David N

    2013-11-01

    Interleukin-17A (IL-17A) is a potent proinflammatory cytokine and the signature cytokine of Th17 cells, a subset which is involved in cytokine and chemokine production, neutrophil recruitment, promotion of T cell priming, and antibody production. IL-17 may play an important role in tuberculosis and other infectious diseases. In preparation for investigating its role in the highly relevant guinea pig model of pulmonary tuberculosis, we cloned guinea pig IL-17A for the first time. The complete coding sequence of the guinea pig IL-17A gene (477 nucleotides; 159 amino acids) was subcloned into a prokaryotic expression vector (pET-30a) resulting in the expression of a 17 kDa recombinant guinea pig IL-17A protein which was confirmed by mass spectrometry analysis. Homology modeling of guinea pig IL-17A revealed that the three-dimensional structure resembles that of human IL-17A. The secondary structure predicted for this protein showed the presence of one extra helix in the N-terminal region. The expression profile of IL-17A was analyzed quantitatively in spleen, lymph node, and lung cells from BCG-vaccinated guinea pigs by real-time PCR. The guinea pig IL-17A cDNA and its recombinant protein will serve as valuable tools for molecular and immunological studies in the guinea pig model of pulmonary TB and other human diseases.

  3. Molecular cloning and expression of the calmodulin gene from guinea pig hearts.

    Science.gov (United States)

    Feng, Rui; Liu, Yan; Sun, Xuefei; Wang, Yan; Hu, Huiyuan; Guo, Feng; Zhao, Jinsheng; Hao, Liying

    2015-06-01

    The aim of the present study was to isolate and characterize a complementary DNA (cDNA) clone encoding the calmodulin (CaM; GenBank accession no. FJ012165) gene from guinea pig hearts. The CaM gene was amplified from cDNA collected from guinea pig hearts and inserted into a pGEM®-T Easy vector. Subsequently, CaM nucleotide and protein sequence similarity analysis was conducted between guinea pigs and other species. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to investigate the CaM 3 expression patterns in different guinea pig tissues. Sequence analysis revealed that the CaM gene isolated from the guinea pig heart had ∼90% sequence identity with the CaM 3 genes in humans, mice and rats. Furthermore, the deduced peptide sequences of CaM 3 in the guinea pig showed 100% homology to the CaM proteins from other species. In addition, the RT-PCR results indicated that CaM 3 was widely and differentially expressed in guinea pigs. In conclusion, the current study provided valuable information with regard to the cloning and expression of CaM 3 in guinea pig hearts. These findings may be helpful for understanding the function of CaM3 and the possible role of CaM3 in cardiovascular diseases.

  4. Comparative analysis of detection methods for congenital cytomegalovirus infection in a Guinea pig model.

    Science.gov (United States)

    Park, Albert H; Mann, David; Error, Marc E; Miller, Matthew; Firpo, Matthew A; Wang, Yong; Alder, Stephen C; Schleiss, Mark R

    2013-01-01

    To assess the validity of the guinea pig as a model for congenital cytomegalovirus (CMV) infection by comparing the effectiveness of detecting the virus by real-time polymerase chain reaction (PCR) in blood, urine, and saliva. Case-control study. Academic research. Eleven pregnant Hartley guinea pigs. Blood, urine, and saliva samples were collected from guinea pig pups delivered from pregnant dams inoculated with guinea pig CMV. These samples were then evaluated for the presence of guinea pig CMV by real-time PCR assuming 100% transmission. Thirty-one pups delivered from 9 inoculated pregnant dams and 8 uninfected control pups underwent testing for guinea pig CMV and for auditory brainstem response hearing loss. Repeated-measures analysis of variance demonstrated no statistically significantly lower weight for the infected pups compared with the noninfected control pups. Six infected pups demonstrated auditory brainstem response hearing loss. The sensitivity and specificity of the real-time PCR assay on saliva samples were 74.2% and 100.0%, respectively. The sensitivity of the real-time PCR on blood and urine samples was significantly lower than that on saliva samples. Real-time PCR assays of blood, urine, and saliva revealed that saliva samples show high sensitivity and specificity for detecting congenital CMV infection in guinea pigs. This finding is consistent with recent screening studies in human newborns. The guinea pig may be a good animal model in which to compare different diagnostic assays for congenital CMV infection.

  5. Generation and Characterization of Inhibitory Antibodies Specific to Guinea Pig CXCR1 and CXCR2.

    Science.gov (United States)

    Tanaka, Kento; Yoshimura, Chigusa; Shiina, Tetsuo; Terauchi, Tomoko; Yoshitomi, Tomomi; Hirahara, Kazuki

    2017-04-01

    CXCR1 and CXCR2 are chemokine receptors that have different selectivity of chemokine ligands, but the distinct role of each receptor is not clearly understood. This is due to the absence of specific inhibitors in guinea pigs, which are the appropriate species for investigation of CXCR1 and CXCR2 because of their functional similarity to humans. In this study, we generated and evaluated monoclonal antibodies that specifically bound to guinea pig CXCR1 (gpCXCR1) and guinea pig CXCR2 (gpCXCR2) for acquisition of specific inhibitors. To assess the activity of antibodies, we established CHO-K1 cells stably expressing either gpCXCR1 or gpCXCR2 (CHO/gpCXCR1 or CHO/gpCXCR2). CHO/gpCXCR1 showed migration in response to guinea pig interleukin (IL)-8, and CHO/gpCXCR2 showed migration in response to both guinea pig IL-8 and guinea pig growth-regulated oncogene α. The receptor selectivities of the chemokines of guinea pigs were the same as the human orthologs. The inhibitory activities of the anti-gpCXCR1 and anti-gpCXCR2 monoclonal antibodies on cell migration were observed in a concentration-dependent manner. In conclusion, we successfully obtained inhibitory antibodies specific to gpCXCR1 and gpCXCR2. These inhibitory antibodies will be useful to clarify the physiological roles of CXCR1 and CXCR2 in guinea pigs.

  6. The guinea pig maximization test--with a multiple dose design

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Vølund, A; Frankild, S

    1995-01-01

    The guinea pig maximization test (GPMT) is usually performed with one moderately irritant induction dose of the allergen and gives a qualitative assessment-hazard identification-of the allergenicity of the chemical. We refined the GPMT by applying a multiple dose design and used 30 guinea pigs...

  7. Cumulative irritancy in the guinea pig from low grade irritant vehicles and the angry skin syndrome

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Maibach, H I

    1980-01-01

    A 4-week open cumulative irritancy test in guinea pigs discriminated between two low grade irritant vehicles, nonionic base (anhydrous) and hydrophilic ointment. The procedure might be useful as a predictive test for low grade irritants. The angry skin syndrome was established in the guinea pigs...

  8. METODOLOGÍAS EN LA ENSEÑANZA DE LA DERIVADA: URACCAN-NUEVA GUINEA

    Directory of Open Access Journals (Sweden)

    William Oswaldo Flores López

    2013-10-01

    Full Text Available Esta investigación describe la metodología que utilizan profesores de la URACCAN -Nueva Guinea, en la enseñanza-aprendizaje de la Derivada a estudiantes de Administración de Empresas, y cómo esta metodología educativa influye en el rendimiento de la asignatura “Matemática Financiera”. Fue un estudio de caso en el que participaron dos profesores de matemática; los datos fueron obtenidos a través de un cuestionario y entrevista abierta; además, una encuesta que se le suministró al alumnado en las que se les solicitó información relacionada a los procesos metodológicos en el aula. El análisis se desarrolló en función de las redes sistémicas y los resultados muestran que existen diferencias significativas a la hora de abordar el contenido, los resultados comparativos favorecen a los estudiantes a quienes se les facilitó  clases en  la enseña de la Derivada con pertinencia al contexto social y en cuanto al profesor que aborda la Derivada desde el enfoque tradicional, se evidencian calificaciones bajas por parte del estudiantado.SummaryThis research describes the methodology that is been used by teachers of URACCAN-Nueva Guinea, in the teaching and learning of the derivatives with students of Business Administration, and how this educational methodology influences the performance of "Financial Mathematics" as a subject. In this case-study two mathematics teachers participated; the data were obtained through a questionnaire and open interviews, as well as a survey that was provided to the students in which they requested information related to the methodological processes in the classroom.  The analysis was developed based on the systemic networks and the results demonstrated significant differences in addressing the content. The comparative results favor students, due to the fact that they were provided with training classes related to the derivative with pertinence to the social context; and as for the teacher who teaches

  9. Evaluación del efecto de la intensidad de labranza en la formación de costra superficial de un oxisol de sabana en los Llanos Orientales de Colombia. II. Caracterización física en superficie

    Directory of Open Access Journals (Sweden)

    Jesús H Galvis

    2007-01-01

    Full Text Available La metodología presentada en este artículo permitió correlacionar el sellamiento del suelo y propiedades físicas como estabilidad estructural, velocidad de infiltración, suelo erosionado, escorrentía, resistencia normal y torsional, y distribución de agregados. Los resultados mostraron que los pastos mejorados después de ocho años de sembrados produjeron una respuesta relativamente positiva del suelo a la erosión y a los esfuerzos mecánicos. Pero la labranza y la fertilización periódicas que demandó la producción de maíz, aunque prepararon relativamente bien la cama de semillas y propiciaron un buen ambiente para el almacenamiento de humedad, permitieron un incremento de la erosión hídrica y un decrecimiento de la estabilidad de la estructura.

  10. Vaccination with Trypanosoma rangeli induces resistance of guinea pigs to virulent Trypanosoma cruzi.

    Science.gov (United States)

    Basso, B; Moretti, E; Fretes, R

    2014-01-15

    Chagas' disease, endemic in Latin America, is spread in natural environments through animal reservoirs, including marsupials, mice and guinea pigs. Farms breeding guinea pigs for food are located in some Latin-American countries with consequent risk of digestive infection. The aim of this work was to study the effect of vaccination with Trypanosoma rangeli in guinea pigs challenged with Trypanosoma cruzi. Animals were vaccinated with fixated epimastigotes of T. rangeli, emulsified with saponin. Controls received only PBS. Before being challenged with T. cruzi, parasitemia, survival rates and histological studies were performed. The vaccinated guinea pigs revealed significantly lower parasitemia than controls (pguinea pigs and dogs. The development of vaccines for use in animals, like domestic dogs and guinea pigs in captivity, opens up new opportunities for preventive tools, and could reduce the risk of infection with T. cruzi in the community. Copyright © 2013 Elsevier B.V. All rights reserved.

  11. [Analysis of the main components of inner ear antigens inducing autoimmune Meniere's disease in guinea pigs].

    Science.gov (United States)

    Lu, Ling; Tan, Chang-Qiang; Cui, Yu-Gui; Ding, Gui-Peng; Ju, Xiao-Bin; Li, Yu-Jin; Cai, Wen-Jun

    2008-08-01

    To investigate the main components of inner ear antigens inducing autoimmune Meniere's disease (AIMD) in guinea pigs. The guinea pigs were immunized with isologous crude inner ear antigens (ICIEAg). Then, the hearing function was measured with auditory brainstem response (ABR), the vestibular function was measured with electronystagmography (including spontaneous nystagmus and caloric test), and inner ear histopathological changes were observed by inner ear celloidin section with haematoxylin-eosin staining and observed under light microscope. According to these results, the AIMD-model animals from non-AIMD-model ones were distinguished. The special antibodies against ICIEAg in sera were measured with ELISA. The antigen-antibody reactions against different components of ICIEAg were detected by Western blotting with sera of AIMD and non-AIMD guinea pigs respectively. Then, we analysed the contrast between them and found the main components of the ICIEAg that were positive reaction in AIMD guinea pigs and negative reaction in non-AIMD guinea pigs. The result of ELISA demonstrated that the sera of both the AIMD and non-AIMD guniea pigs contained the special antibodies against ICIEAg after immunized with ICIEAg. The difference of the amount of antibody against ICIEAg between AIMD guinea pig group and non-AIMD guinea pig group was not significant. Western blotting assay showed only the sera of AIMD guinea pig contained the antibodies against the specific antigens with the molecular of 68 000, 58 000, 42 000 and 28 000. ICIEAg contain many different components, the AIMD might only happen in the guinea pigs in which the special immunization against the main components that could induce this kind of disorder appeared. The inner ear antigens with molecular of 68 000, 58 000, 42 000 and 28 000 might be the main components inducing AIMD in guinea pigs.

  12. A First Generation Comparative Chromosome Map between Guinea Pig (Cavia porcellus) and Humans.

    Science.gov (United States)

    Romanenko, Svetlana A; Perelman, Polina L; Trifonov, Vladimir A; Serdyukova, Natalia A; Li, Tangliang; Fu, Beiyuan; O'Brien, Patricia C M; Ng, Bee L; Nie, Wenhui; Liehr, Thomas; Stanyon, Roscoe; Graphodatsky, Alexander S; Yang, Fengtang

    2015-01-01

    The domesticated guinea pig, Cavia porcellus (Hystricomorpha, Rodentia), is an important laboratory species and a model for a number of human diseases. Nevertheless, genomic tools for this species are lacking; even its karyotype is poorly characterized. The guinea pig belongs to Hystricomorpha, a widespread and important group of rodents; so far the chromosomes of guinea pigs have not been compared with that of other hystricomorph species or with any other mammals. We generated full sets of chromosome-specific painting probes for the guinea pig by flow sorting and microdissection, and for the first time, mapped the chromosomal homologies between guinea pig and human by reciprocal chromosome painting. Our data demonstrate that the guinea pig karyotype has undergone extensive rearrangements: 78 synteny-conserved human autosomal segments were delimited in the guinea pig genome. The high rate of genome evolution in the guinea pig may explain why the HSA7/16 and HSA16/19 associations presumed ancestral for eutherians and the three syntenic associations (HSA1/10, 3/19, and 9/11) considered ancestral for rodents were not found in C. porcellus. The comparative chromosome map presented here is a starting point for further development of physical and genetic maps of the guinea pig as well as an aid for genome assembly assignment to specific chromosomes. Furthermore, the comparative mapping will allow a transfer of gene map data from other species. The probes developed here provide a genomic toolkit, which will make the guinea pig a key species to unravel the evolutionary biology of the Hystricomorph rodents.

  13. El sector agrario del Ecuador: incertidumbres (riesgos ante la globalización

    Directory of Open Access Journals (Sweden)

    García Pascual, Francisco

    2006-01-01

    Full Text Available El artículo ofrece una visión de conjunto del sector agrario ecuatoriano. Incorpora, por una parte, el escenario esbozado por las conflictivas coyunturas políticas y económicas por las que ha atravesado el país. Por otra, el rechazo a cualquier imagen de inmovilismo o de homogeneización de la agricultura y/o del espacio rural ecuatoriano. Plantea asumir los retos y las incertidumbres que la globalización neoliberal impone al mundo agrario y al mundo rural del Ecuador, desde la perspectiva de las respuestas locales a los impactos derivados de procesos globales. En este sentido, entre 1980 y 2005, el sector agrario ecuatoriano ha dibujado una trayectoria marcada por cuatro hechos: a la consolidación de unas estructuras que siguen siendo muy desequilibradas social y territorialmente, b una ralentización del crecimiento de la frontera agrícola, un aumento de las tierras dedicadas a pastos, una reorientación de las tierras cultivadas en beneficio especialmente de ítems exportadores o de demanda masiva urbana, y un incremento considerable de la actividad ganadera, c una notable alza de la producción física junto a un significativo decrecimiento de los precios percibidos por los agricultores en términos reales, y d una notable expansión de las exportaciones agrícolas, lo que ha posible merced a una sensible disminución de los precios unitarios de los bienes agroalimentarios vendidos al exterior.

  14. Ozone-Induced Hypertussive Responses in Rabbits and Guinea Pigs

    Science.gov (United States)

    Clay, Emlyn; Patacchini, Riccardo; Trevisani, Marcello; Preti, Delia; Branà, Maria Pia; Spina, Domenico

    2016-01-01

    Cough remains a major unmet clinical need, and preclinical animal models are not predictive for new antitussive agents. We have investigated the mechanisms and pharmacological sensitivity of ozone-induced hypertussive responses in rabbits and guinea pigs. Ozone induced a significant increase in cough frequency and a decrease in time to first cough to inhaled citric acid in both conscious guinea pigs and rabbits. This response was inhibited by the established antitussive drugs codeine and levodropropizine. In contrast to the guinea pig, hypertussive responses in the rabbit were not inhibited by bronchodilator drugs (β2 agonists or muscarinic receptor antagonists), suggesting that the observed hypertussive state was not secondary to bronchoconstriction in this species. The ozone-induced hypertussive response in the rabbit was inhibited by chronic pretreatment with capsaicin, suggestive of a sensitization of airway sensory nerve fibers. However, we could find no evidence for a role of TRPA1 in this response, suggesting that ozone was not sensitizing airway sensory nerves via activation of this receptor. Whereas the ozone-induced hypertussive response was accompanied by a significant influx of neutrophils into the airway, the hypertussive response was not inhibited by the anti-inflammatory phosphodiesterase 4 inhibitor roflumilast at a dose that clearly exhibited anti-inflammatory activity. In summary, our results suggest that ozone-induced hypertussive responses to citric acid may provide a useful model for the investigation of novel drugs for the treatment of cough, but some important differences were noted between the two species with respect to sensitivity to bronchodilator drugs. PMID:26837703

  15. Sequía, inmigración y políticas locales: el Sahel en la encrucijada del desarrollo

    Directory of Open Access Journals (Sweden)

    Sara Nso

    2008-10-01

    Full Text Available Este artículo trata de delimitar los principales factores socio-económicos y políticos que ponen en relación desertificación y flujos migratorios internacionales, en el área Sahel. A través del estudio -no exhaustivo para todos los países de la muestra, por ausencia de datos disponibles- de dichas realidades en Burkina Faso, Gambia, Guinea-Bissau, Guinea, Malí, Mauritania, Níger y Senegal, el análisis del elemento local será privilegiado, sin despreciar por ello el valor de las dinámicas regionales. Desde una perspectiva "transnacionalista", se aportarán así las claves para la comprensión global de la problemática saheliana, con el objetivo de sacar a esta región de la encrucijada del subdesarrollo.

  16. Microbial flora of odontogenic abscesses in pet guinea pigs.

    Science.gov (United States)

    Minarikova, A; Hauptman, K; Knotek, Z; Jekl, V

    2016-10-01

    Abscesses of odontogenic origin in guinea pigs pose a serious health problem and need to be treated with a combination of surgical and medical therapy. The aim of this prospective study was to describe the microbial flora of odontogenic abscesses associated with osteomyelitis in 24 pet guinea pigs, to perform antibiotic sensitivity testing, and to make recommendations for practitioners on the antibiotics of first choice. Inclusion criteria for the study included the animal being diagnosed with an odontogenic abscess which underwent surgery and was not pre-treated with an antibiotic. Inclusion criteria matched for 24 guinea pigs. Samples (pus, capsule and affected tooth/bone) for bacteriological examination were collected under sterile conditions during the surgical procedure. The most commonly isolated bacteria from abscesses of odontogenic origin were Bacteroides fragilis in 12.8 per cent (6/47) of cases, Pasteurella multocida in 10.6 per cent (5/47) and Peptostreptococcus anaerobius in 8.5 per cent (4/47). Aerobic bacterial species only were isolated in 29.2 per cent (7/24) of cases, anaerobic bacteria only were isolated in 33.3 per cent (8/24), and mixed infection with anaerobic and aerobic bacterial species was seen in 37.5 per cent (9/24). Aerobes (n=20) were sensitive to enrofloxacin and marbofloxacin in 100 per cent of samples, benzylpenicillin potassium (penicillin G, PNCG) in 90 per cent, cephalotin in 85 per cent, amoxicillin-clavulanate in 75 per cent, doxycycline in 70 per cent, gentamicin in 65 per cent and trimethoprim-sulfamethoxazole in 55 per cent. Anaerobes (n=27) were sensitive to amoxicillin-clavulanate in 100 per cent of cases, clindamycin in 96.3 per cent, metronidazole in 92.6 per cent, PNCG in 92.6 per cent and cephalotin in 74.1 per cent. As guinea pigs are strictly herbivorous animals, based on the results of this study the recommended antibiotic treatment for odontogenic abscesses is a combination of fluoroquinolones and metronidazole

  17. Welding in the highlands of Papua New Guinea

    International Nuclear Information System (INIS)

    Dinon, Mike; Mendez, Alfredo; Ward, Jason

    2007-01-01

    This paper gives examples of the qualification of welding procedures intended for use in the highlands of Papua New Guinea. Two examples are given of welding procedures which required the development of a unique approach in each case after an early attempt using inherited standard procedures failed. The main conclusions are the requirement for caution when converting ASMEIX welding procedures for use with AS 2885.2, the requirement under the Australian Code to 'design out' the risk of hydrogen assisted cold cracking, and the importance of choosing an upper-end carbon equivalent when welding high carbon equivalent fittings

  18. Antitussive and antibronchoconstriction actions of fenspiride in guinea-pigs.

    Science.gov (United States)

    Laude, E A; Bee, D; Crambes, O; Howard, P

    1995-10-01

    Fenspiride is a nonsteroidal anti-inflammatory agent, which we have previously shown to have an in vivo antibronchoconstrictor action in guinea pigs. We have currently studied this action using the constrictors Substance P, neurokinin A, citric acid and capsaicin in anaesthetized guinea-pigs. Fenspiride has also been reported to produce a subjective improvement in cough in patients. We have used a conscious guinea-pig model of cough as a more definitive method to study the effect of fenspiride on capsaicin- and citric acid-induced cough. Aerosolized fenspiride (1 mg.mL-1) caused a 58% reversal of capsaicin-induced bronchoconstriction; and i.v. fenspiride (1mg.kg-1) a 45% reversal of citric acid induced bronchoconstriction. Substance P- and neurokinin A-induced bronchoconstriction were unaffected by 1 mg.kg-1 i.v. fenspiride. Aerosolized fenspiride (1, 3 and 10 mg.mL-1) administered for 4 min reduced citric acid (300 mM) induced cough, but 0.1 mg.mL-1 was without effect. Pretreatment with aerosolized fenspiride (10 mg.mL-1) caused a shift in the citric acid dose response curve to the right. For citric acid-induced cough, the duration of action of aerosolized fenspiride (10 mg.mL-1) was found to be 5 and 15 min post-treatment. Aerosolized capsaicin (30 microM) induced cough was also reduced by 3 and 10 mg.mL-1 aerosolized fenspiride, but no significant effect was found with 1 mg.mL-1. We conclude that aerosolized fenspiride reduces capsaicin- and citric acid-induced bronchoconstriction as well as induced cough in guinea-pigs in vivo. Whether a pathway common to both cough and bronchoconstriction is the site of action of fenspiride remains to be established. We postulate that fenspiride, acting as an antitussive and antibronchoconstrictor agent, would be beneficial in the clinical situation for those patients with hyperresponsive airways.

  19. Prostaglandin release from in vitro guinea-pig gallbladder.

    Science.gov (United States)

    Booker, M L; LaMorte, W W

    1983-02-01

    In order to study prostaglandin release from guinea pig gallbladder, full thickness tissue sections were incubated for one hour in Krebs solution. Extraction and two dimensional chromatography of incubation media obtained in the presence of radio-labelled arachidonic acid demonstrated the presence of PGE2, PGF2 alpha, 6-keto-PGF1 alpha and thromboxane B2. These results were supported by radioimmunoassay of incubations conducted in the absence of exogenous arachidonate and in the presence of varying concentrations of unlabelled exogenous arachidonate. The previously reported predominance of PGE2 was only seen at high concentrations of exogenous arachidonate.

  20. Helicopter-supported drilling operation in Papua New Guinea

    International Nuclear Information System (INIS)

    Wagner, E.R.; Juneau, M.S.

    1991-01-01

    This paper reports on drilling cost per foot of Chevron's helilift drilling operation in the remote Southern Highlands of Papua New Guinea, reduced from 1360 to 267 S/ft (4462 to 876$/m) during the period from 1985 to 1989. The operation provides many challenges, as it is thousands of miles from major oil-field supply centers. This requires advanced will-planning and logistical management of drilling materials so that they arrive at the drilling rig in a timely manner. The wells are also drilled into structurally complex geology without the aid of seismic data which can lead to unexpected results

  1. Hyperthyroidism in four guinea pigs: clinical manifestations, diagnosis, and treatment.

    Science.gov (United States)

    Künzel, F; Hierlmeier, B; Christian, M; Reifinger, M

    2013-12-01

    Hyperthyroidism was diagnosed in four guinea pigs by demonstration of an increased serum total thyroxine concentration. The main clinical signs were comparable with those observed in feline hyperthyroidism and included weight loss despite maintenance of appetite and a palpable mass in the ventral cervical region. Three animals were treated successfully with methimazole for between 13 and 28 months. Clinical signs and regular measurement of circulating total thyroxine concentrations appear to be convenient parameters for monitoring response to medical therapy. © 2013 British Small Animal Veterinary Association.

  2. Changes in guinea-pig dermal collagen during development

    International Nuclear Information System (INIS)

    Shuttleworth, C.A.; Forrest, L.

    1975-01-01

    Guinea-pig dermis was digested with pepsin and the solubilized collagen molecules separated by differential salt precipitation at pH 7.5. Differences in subunit composition and amino acid analysis were noted between type I and type III collagen. Incorporation of radioactive proline into the developing foetus enabled isolation of labelled type I and type III collagens. Comparison of the specific activity of the isolated collagen molecules showed that type III collagen had a high specific activity in the early stages of foetal development, which decreased dramatically during foetal development. The specific activity of pepsin-solubilized type I collagen remained fairly constant during foetal development. (orig.) [de

  3. Molecular cloning and expression of the IL-10 gene from guinea pigs.

    Science.gov (United States)

    Dirisala, Vijaya R; Jeevan, Amminikutty; Bix, Gregory; Yoshimura, Teizo; McMurray, David N

    2012-04-25

    The Guinea pig (Cavia porcellus) is one of the most relevant small animals for modeling human tuberculosis (TB) in terms of susceptibility to low dose aerosol infection, the organization of granulomas, extrapulmonary dissemination and vaccine-induced protection. It is also considered to be a gold standard for a number of other infectious and non-infectious diseases; however, this animal model has a major disadvantage due to the lack of readily available immunological reagents. In the present study, we successfully cloned a cDNA for the critical Th2 cytokine, interleukin-10 (IL-10), from inbred Strain 2 guinea pigs using the DNA sequence information provided by the genome project. The complete open reading frame (ORF) consists of 537 base pairs which encodes a protein of 179 amino acids. This cDNA sequence exhibited 87% homology with human IL-10. Surprisingly, it showed only 84% homology with the previously published IL-10 sequence from the C4-deficient (C4D) guinea pig, leading us to clone IL-10 cDNA from the Hartley strain of guinea pig. The IL-10 gene from the Hartley strain showed 100% homology with the IL-10 sequence of Strain 2 guinea pigs. In order to validate the only published IL-10 sequence existing in Genbank reported from C4D guinea pigs, genomic DNA was isolated from tissues of C4D guinea pigs. Amplification with various sets of primers showed that the IL-10 sequence reported from C4D guinea pigs contained numerous errors. Hence the IL-10 sequence that is being reported by us replaces the earlier sequence making our IL-10 sequence to be the first one accurate from guinea pig. Recombinant guinea pig IL-10 proteins were subsequently expressed in both prokaryotic and eukaryotic cells, purified and were confirmed by N-terminal sequencing. Polyclonal anti-IL-10 antibodies were generated in rabbits using the recombinant IL-10 protein expressed in this study. Taken together, our results indicate that the DNA sequence information provided by the genome project

  4. Variables asociadas con el uso de hábitat del manatí del Caribe (Trichechus manatus, en Quintana Roo, México (Mammalia

    Directory of Open Access Journals (Sweden)

    Javier Axis-Arroyo

    1998-09-01

    Full Text Available La influencia de: temperatura atmosférica y del agua, vientos, nubosidad, profundidad, salinidad, abundancia de pastos y algas, y estructura de grupo; en la distribución espacial de Trichechus manatus manatus, fue estudiada en la Bahía de Chetumal, Quintana Roo, México; de noviembre de 1994 a junio de 1995. Las variables con menor asociación fueron: nubosidad y temperatura atmosférica y del agua (en contraste con lo reportado para la subespecie de Florida, Trichechus manatus latirostris; las variables con asociación moderada fueron: salinidad (como en Florida, profundidad (uso frecuente de profundidades entre 0.80 y 2 m y estructura de grupo (lo cual sugiere que la zona no es importante como área de apareamiento. La distribución espacial fue asociada principalmente con cambios drásticos en la intensidad del viento y con el alimento disponible (similar a trabajos previos realizados en México.Influence of atmospheric variables and water temperature, winds, cloudiness, depth, salinity, grass and algal abundance, and group structure on the spatial distribution of Trichechus manatus manatus was studied in Chetumal Bay, Quintana Roo, Mexico. Boat surveys were done from November 1994 trough June 1995. There was little association with cloudiness and atmospheric and water temperature (in contrast with reports for the Florida subspecies, Trichechus manatus latirostris; the variables with moderate association were salinity (as in Florida, depth (frequent use of depths between 0.80-2 m and group structure (which suggests that the zone is not an important mating area. Spatial distribution was more associated with drastic changes in wind intensity and the available food (similar to previous works in Mexico.

  5. Evaluación del establecimiento de Leucaena leucocephala cv. Cunningham en una finca ganadera del municipio de Perico, Matanzas, Cuba

    Directory of Open Access Journals (Sweden)

    Katia Bover

    Full Text Available El objetivo de este trabajo fue evaluar el establecimiento de Leucaena leucocephala cv. Cunningham en una finca de la cooperativa de créditos y servicios (CCS Ramón Rodríguez Milián, del municipio de Perico. Se trabajó en un área de 6 ha, dividida en cinco cuartones, y se realizó una preparación en franjas de 2 m de ancho. Se determinó la supervivencia después del trasplante, la altura, el número de ramas y la composición florística del pastizal; así como el efecto de las labores de limpieza y el daño causado por los animales en la altura. Se aplicó un análisis de varianza y los datos se procesaron con el paquete estadístico SPSS® versión 10.0 para Windows XP. La supervivencia de las plantas fue de 98 %; la labor de limpieza tuvo un efecto positivo en el crecimiento, mientras que la defoliación de la planta por los animales afectó el establecimiento. La población de pastos naturales (jiribilla y pitilla se mantuvo alrededor de 66 % y la de las principales leguminosas volubles (teramnus, glycine y siratro disminuyó de 12 % en el tercer mes después de realizado el trasplante, a 6 % en el decimoquinto mes. El sistema se consideró establecido cuando las plantas sobrepasaron los 2 m de altura. Se concluye que la labor de limpieza influyó positivamente en el comportamiento de la altura y el establecimiento de L. leucocephala, mientras que las defoliaciones causadas por los animales lo retrasaron.

  6. Efecto del género y etapa de crecimiento sobre la composición química y mineral de pastos tropicales utilizados para alimentar vacas lecheras

    DEFF Research Database (Denmark)

    Arzate-Vázquez, Gerardo L.; Castrejón-Pineda, Francisco A.; Rosiles-Martínez, René

    2016-01-01

    In tropical production systems, dairy cows are rarely supplemented with minerals, which often lead to mineral imbalances. Grasses grown together in the same soil type and sampled at the same growth stage can vary widely in chemical and mineral composition; therefore, the objective of this study w...

  7. Epidemiology of chronic kidney diseases in the Republic of Guinea; future dialysis needs.

    Science.gov (United States)

    Bah, Alpha Oumar; Lamine, Cisse; Balde, Mamadou Cellou; Bah, Mamadou Lamine Yaya; Rostaing, Lionel

    2015-10-01

    Chronic kidney disease (CKD) is increasing worldwide and can lead to end-stage renal disease (ESRD). Because few patients with ESRD in the Republic of Guinea have access to haemodialysis, we retrospectively evaluated the prevalence of CKD, ESRD and access to supportive therapies. 579 CKD patients (304 males; mean age: 44 ± 16 years) were admitted into Conakry nephrology department, the only centre in the Republic of Guinea, between 2009 and 2013. Most patients (63%) resided within Conakry (the capital), 12.5% came from lower Guinea, 11.7% from middle Guinea, 7.9% from upper Guinea and 4.8% from forest Guinea. Reasons for referral were increased serum creatinine (49.5%), hypertension (27%) and diffuse edema (17%). Also, 11% were diabetic, 12.5% were smokers, 17% were HIV-positive, 8.3% were HBV-positive and 15% were HCV-positive. The most frequent symptom at admission was nausea/vomiting (56%). Upon admission, 70.5% of patients already had ESRD. Although no kidney biopsies were performed it was assumed that 34% and 27% of patients had vascular nephropathy and chronic glomerulonephritis, respectively. Of the 385 ESRD patients, only 140 (36.3%) had access to haemodialysis (two sessions/week, 4 hours each). Most patients that received haemodialysis resided within the Conakry region (P Conakry, plus four new nephrology/haemodialysis centres within the Republic of Guinea, each holding ≥30 haemodialysis machines.

  8. Clinical signs, therapy and zoonotic risk of pet guinea pigs with dermatophytosis.

    Science.gov (United States)

    Kraemer, A; Hein, J; Heusinger, A; Mueller, R S

    2013-03-01

    Systematic studies about pet guinea pigs with dermatophytosis are rare. The aim of this study was to evaluate clinical signs, therapy and zoonotic risk of pet guinea pigs with dermatophytosis. Questionnaires from both owners (n = 74) of pet guinea pigs with dermatophytosis and their veterinarians (n = 101) were analysed regarding clinical signs, therapy and data pertinent to zoonotic potential. Trichophyton (T.) mentagrophytes was found in 97% of cases. In the weeks preceding the onset of the clinical signs, a new guinea pig joined the household in 43% of cases. One third of the affected guinea pigs had lived in the household for less than 3 months. Predominant clinical signs were alopecia (83%), scaling (73%) and crusting (70%). The most commonly affected body site was the head (75%). In approximately one quarter of the cases humans showed clinical signs of dermatophytosis, in half the households, only children were affected. Skin lesions were seen most often on the face, the neck and the arms. Pet guinea pigs carrying dermatophytes must be considered a serious zoonotic risk for their owners, especially for children. A major risk factor for dermatophytosis seems to be a recent acquisition of a new guinea pig. © 2012 Blackwell Verlag GmbH.

  9. Prevalence of dermatophytes and other superficial fungal organisms in asymptomatic guinea pigs in Southern Italy.

    Science.gov (United States)

    d'Ovidio, D; Grable, S L; Ferrara, M; Santoro, D

    2014-07-01

    Guinea pigs have been indicated as a potential source of zoophilic dermatophytes that cause human dermatomycosis. The purpose of this study was to evaluate the prevalence of dermatophytes as well as saprophytic fungi in asymptomatic pet guinea pigs in Southern Italy. Two-hundred pet guinea pigs were enrolled from both private veterinary clinics and pet shops in the Campania region, Italy, from August 2012 to September 2013. Samples were collected using the MacKenzie's toothbrush technique. The plates were incubated for four weeks at 25°C and identification of the fungal colonies was based on both macroscopic and microscopic characteristics. Two pathogenic dermatophytes were isolated in 9 (4·5%) of 200 guinea pigs; Epidermophyton species in 2 (1%) and Scopulariopsis species in 7 (3·5%). Saprophytic dermatophytes were isolated from 151 (75·5%) animals enrolled. No fungal growth was observed in 40 (20%) guinea pigs. The results of this study indicate a low prevalence of pathogenic dermatophytes in pet guinea pigs in Southern Italy but the presence of Epidermophyton and Scopulariopsis species in asymptomatic pet guinea pigs. © 2014 British Small Animal Veterinary Association.

  10. Temperature Preference in IAF Hairless and Hartley Guinea Pigs (Cavia porcellus).

    Science.gov (United States)

    Kleven, Gale A; Joshi, Prianca

    2016-03-01

    The Hairless strain of guinea pigs (Cavia porcellus) is the result of a spontaneous recessive mutation first identified at the Institute Armand Frappier (IAF) in 1978. Despite the longstanding availability of this strain, little is known about its thermoregulatory behavior. The aim of this study was to determine temperature preference in Hartley and Hairless guinea pigs by observing each strain in a ring-shaped apparatus containing a nonlinear temperature gradient. Temperatures were maintained by separately controlled heating mats lining the apparatus. Set point temperatures ranged from 24 to 38 °C. Guinea pigs (Hartley female, Hairless female, and Hairless male guinea pigs; n = 8 each group) were placed either singly or in pairs at 1 of the 8 randomized starting points within the apparatus. Subjects were observed for 30 min and coded for location within the temperature gradient by both frequency and duration. When placed singly in the apparatus, all 3 groups spent more time in the 30 °C zones. However, when placed as pairs with a cagemate, Hartley female guinea pigs spent more time in the cooler range of temperatures from 24 to 30 °C, whereas Hairless guinea pigs preferred a range of 30 to 38 °C. These results confirm a temperature preference of 30 ± 2 °C for both Hartley and Hairless guinea pigs when singly housed. However, data from the paired housing condition suggest that context plays an important role in thermoregulatory behavior.

  11. Investigation Into the Humaneness of Slaughter Methods for Guinea Pigs (Cavia porcelus) in the Andean Region.

    Science.gov (United States)

    Limon, Georgina; Gonzales-Gustavson, Eloy A; Gibson, Troy J

    2016-01-01

    Guinea pigs (Cavia porcelus) are an important source of nonhuman animal protein in the Andean region of South America. Specific guidelines regarding the welfare of guinea pigs before and during slaughter have yet to be developed. This study critically assessed the humaneness of 4 different stunning/slaughter methods for guinea pigs: cervical neck dislocation (n = 60), electrical head-only stunning (n = 83), carbon dioxide (CO2) stunning (n = 21), and penetrating captive bolt (n = 10). Following cervical neck dislocation, 97% of guinea pigs had at least 1 behavioral or cranial/spinal response. Six percent of guinea pigs were classified as mis-stunned after electrical stunning, and 1% were classified as mis-stunned after captive bolt. Increased respiratory effort was observed during CO2 stunning. Apart from this finding, there were no other obvious behavioral responses that could be associated with suffering. Of the methods assessed, captive bolt was deemed the most humane, effective, and practical method of stunning guinea pigs. Cervical neck dislocation should not be recommended as a slaughter method for guinea pigs.

  12. Guinea pig model for evaluating the potential public health risk of swine and avian influenza viruses.

    Directory of Open Access Journals (Sweden)

    Yipeng Sun

    Full Text Available BACKGROUND: The influenza viruses circulating in animals sporadically transmit to humans and pose pandemic threats. Animal models to evaluate the potential public health risk potential of these viruses are needed. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the guinea pig as a mammalian model for the study of the replication and transmission characteristics of selected swine H1N1, H1N2, H3N2 and avian H9N2 influenza viruses, compared to those of pandemic (H1N1 2009 and seasonal human H1N1, H3N2 influenza viruses. The swine and avian influenza viruses investigated were restricted to the respiratory system of guinea pigs and shed at high titers in nasal tracts without prior adaptation, similar to human strains. None of the swine and avian influenza viruses showed transmissibility among guinea pigs; in contrast, pandemic (H1N1 2009 virus transmitted from infected guinea pigs to all animals and seasonal human influenza viruses could also horizontally transmit in guinea pigs. The analysis of the receptor distribution in the guinea pig respiratory tissues by lectin histochemistry indicated that both SAα2,3-Gal and SAα2,6-Gal receptors widely presented in the nasal tract and the trachea, while SAα2,3-Gal receptor was the main receptor in the lung. CONCLUSIONS/SIGNIFICANCE: We propose that the guinea pig could serve as a useful mammalian model to evaluate the potential public health threat of swine and avian influenza viruses.

  13. Detection of antibodies against Theiler's murine encephalomyelitis virus GDVII strain in experimental guinea pigs.

    Science.gov (United States)

    Häger, C; Glage, S; Held, N; Bleich, E M; Burghard, A; Mähler, M; Bleich, André

    2016-10-01

    A disease affecting guinea pigs called 'guinea pig lameness' characterized by clinical signs of depression, lameness of limbs, flaccid paralysis, weight loss and death within a few weeks was first described by Römer in 1911. After a research group in our facility kept laboratory guinea pigs from two different origins together in one room, lameness was observed in two animals. Further investigations revealed a serological immune response against Theiler's murine encephalomyelitis virus (TMEV; GDVII strain) in these animals. Histopathology of the lumbar spinal cord of these animals showed mononuclear cell infiltration and necrotic neurons in the anterior horn. Therefore, all guinea pigs from this contaminated animal unit, from other units in our facility, as well as from different European institutions and breeding centres were screened for antibodies directed against GDVII. Our investigations showed that approximately 80% of all guinea pigs from the contaminated animal unit were seropositive for GDVII, whereas animals from other separate units were completely negative. In addition, 43% of tested sera from the different European institutions and breeding centres contained antibodies against GDVII. The present data confirm that an unknown viral infection causes an immune response in experimental guinea pigs leading to seroconversion against GDVII and that guinea pigs from a commercial breeder are the source of the infection. © The Author(s) 2015.

  14. Hairless pigmented guinea pigs: a new model for the study of mammalian pigmentation.

    Science.gov (United States)

    Bolognia, J L; Murray, M S; Pawelek, J M

    1990-09-01

    A stock of hairless pigmented guinea pigs was developed to facilitate studies of mammalian pigmentation. This stock combines the convenience of a hairless animal with a pigmentary system that is similar to human skin. In both human and guinea pig skin, active melanocytes are located in the basal layer of the interfollicular epidermis. Hairless albino guinea pigs on an outbred Hartley background (CrI:IAF/HA(hr/hr)BR; designated hr/hr) were mated with red-haired guinea pigs (designated Hr/Hr). Red-haired heterozygotes from the F1 generation (Hr/hr) were then mated with each other or with hairless albino guinea pigs. The F2 generation included hairless pigmented guinea pigs that retained their interfollicular epidermal melanocytes and whose skin was red-brown in color. Following UV irradiation, there was an increase in cutaneous pigmentation as well as an increase in the number of active epidermal melanocytes. An additional strain of black hairless guinea pigs was developed using black Hr/Hr animals and a similar breeding scheme. These two strains should serve as useful models for studies of the mammalian pigment system.

  15. Guinea pig model for evaluating the potential public health risk of swine and avian influenza viruses.

    Science.gov (United States)

    Sun, Yipeng; Bi, Yuhai; Pu, Juan; Hu, Yanxin; Wang, Jingjing; Gao, Huijie; Liu, Linqing; Xu, Qi; Tan, Yuanyuan; Liu, Mengda; Guo, Xin; Yang, Hanchun; Liu, Jinhua

    2010-11-23

    The influenza viruses circulating in animals sporadically transmit to humans and pose pandemic threats. Animal models to evaluate the potential public health risk potential of these viruses are needed. We investigated the guinea pig as a mammalian model for the study of the replication and transmission characteristics of selected swine H1N1, H1N2, H3N2 and avian H9N2 influenza viruses, compared to those of pandemic (H1N1) 2009 and seasonal human H1N1, H3N2 influenza viruses. The swine and avian influenza viruses investigated were restricted to the respiratory system of guinea pigs and shed at high titers in nasal tracts without prior adaptation, similar to human strains. None of the swine and avian influenza viruses showed transmissibility among guinea pigs; in contrast, pandemic (H1N1) 2009 virus transmitted from infected guinea pigs to all animals and seasonal human influenza viruses could also horizontally transmit in guinea pigs. The analysis of the receptor distribution in the guinea pig respiratory tissues by lectin histochemistry indicated that both SAα2,3-Gal and SAα2,6-Gal receptors widely presented in the nasal tract and the trachea, while SAα2,3-Gal receptor was the main receptor in the lung. We propose that the guinea pig could serve as a useful mammalian model to evaluate the potential public health threat of swine and avian influenza viruses.

  16. Tamsulosin modulates, but does not abolish the spontaneous activity in the guinea pig prostate gland.

    Science.gov (United States)

    Chakrabarty, Basu; Dey, Anupa; Lam, Michelle; Ventura, Sabatino; Exintaris, Betty

    2015-06-01

    To examine the effects of the α1A -adrenoceptor antagonist, tamsulosin, on spontaneous contractile and electrical activity in the guinea-pig prostate gland. The effects of tamsulosin (0.1 and 0.3 nM) were investigated in adult and ageing male guinea pig prostate glands using conventional tension recording and electrophysiological intracellular microelectrode recording techniques. Tamsulosin reduced spontaneous activity, and had different age-dependent effects on adult and ageing guinea pigs at different concentrations. 0.1 nM tamsulosin caused a significantly greater reduction of spontaneous contractile and electrical activity in ageing guinea pigs in comparison to adult guinea pigs. In contrast, 0.3 nM tamsulosin had a significantly greater reduction of spontaneous contractile and electrical activity in adult guinea pigs in comparison to ageing guinea pigs. This study demonstrates that tamsulosin can modulate spontaneous myogenic stromal contractility and the underlying spontaneous electrical activity; tamsulosin does not block spontaneous activity. This reduction in spontaneous activity suggests that downstream cellular mechanisms underlying smooth muscle tone are being targeted, and these may represent novel therapeutic targets to better treat benign prostatic hyperplasia. © 2014 Wiley Periodicals, Inc.

  17. The provision of potable water in eradication of Guinea worm infection in Ezza North, Southeastern, Nigeria.

    Science.gov (United States)

    Ede, Alison Okorie; Nwaokoro, Joakin Chidozie; Iwuala, C C; Amadi, A N; Akpelu, Ugochinyere Alvana

    2014-10-01

    Guinea worm is a parasite found in unprotected drinking water sources, causes considerable morbidity and loss of agricultural production among rural people. The study was to determine the current status of Guinea worm infection in Ezza North and to evaluate the impact of control measures on guinea worm infection. A total of 200 individuals in Ezza North Southeastern, Nigeria were examined for guinea worm infection. A standardized questionnaire was used to determine the effect of potable water on guinea worm eradication/control, the source of drinking water, information on the knowledge, attitude, symptom management practices, availability of health facilities and boreholes installation status. The instrument for data collection was well constructed, validated and reliable tested questionnaire by an expert. Data obtained was analyzed using Epi-Info model 3.4 versions. Results of a study indicated majority of the respondents 195 (97.5 %) have access to safe drinking water supply which indicated no case of Guinea worm infection. The active use of potable water supply was found among the age group of 20-30 years 71 (35.5 %) and higher in male (57.5 %) than females (42.5 %). The drastic reduction of Guinea worm infection to zero (0) level in Ezza North were due to multiple factors as health education, availability of functional boreholes, presence of health centers for immediate treatment if any case discovered.

  18. Measurement of guinea pig eosinophil major basic protein by radioimmunoassay

    International Nuclear Information System (INIS)

    Wassom, D.L.; Loegering, D.A.; Gleich, G.J.

    1979-01-01

    Guinea pig eosinophil major basic protein (MBP) was measured by radioimmunoassay (RIA) using 131 I-MBP. Two critical features of the assay were: (1) alkylation of the MBP with iodoacetamide prior to radioiodination and (2) inclusion of another basic protein, either protamine or histone, in the phosphate buffer. Freshly isolated non-alkylated MBP was immunologically deficient when compared to alkylated or reduced MBP, but its reactivity could be redtores by reduction with dithiothreitol and alkylation. Reduction and alkylation also restored the immunoreactivity of polymerized MBP. MBP levels were not elevated in sera from guinea pigs parasitized with Trichinella spiralis and having peripheral blood eosinophilia. Muscle extracts from Trichinella infected animals showed significantly higher levels of MBP activity than normal controls. MBP was measurable in extracts of untreated eosinophils, but reduction and alkylation of these extracts increased MBP activity several fold. The RIA permits detection of MBP in body fluids and tissues at levels as low as 2 ng./ml. The RIA is useful in assessing increased or decreased levels of MBP activity in samples from experimental animals when compared to samples from controls. (author)

  19. ACTION OF DIPHTHERIA TOXIN IN THE GUINEA PIG

    Science.gov (United States)

    Baseman, Joel B.; Pappenheimer, A. M.; Gill, D. M.; Harper, Annabel A.

    1970-01-01

    The blood clearance and distribution in the tissues of 125I after intravenous injection of small doses (1.5–5 MLD or 0.08–0.25 µg) of 125I-labeled diphtheria toxin has been followed in guinea pigs and rabbits and compared with the fate of equivalent amounts of injected 125I-labeled toxoid and bovine serum albumin. Toxoid disappeared most rapidly from the blood stream and label accumulated and was retained in liver, spleen, and especially in kidney. Both toxin and BSA behaved differently. Label was found widely distributed among all the organs except the nervous system and its rate of disappearance from the tissues paralleled its disappearance from the circulation. There was no evidence for any particular affinity of toxin for muscle tissue or for a "target" organ. Previous reports by others that toxin causes specific and selective impairment of protein synthesis in muscle tissue were not confirmed. On the contrary, both in guinea pigs and rabbits, a reduced rate of protein synthesis was observed in all tissues that had taken up the toxin label. In tissues removed from intoxicated animals of both species there was an associated reduction in aminoacyl transferase 2 content. It is concluded that the primary action of diphtheria toxin in the living animal is to effect the inactivation of aminoacyl transferase 2. The resulting inhibition in rate of protein synthesis leads to morphologic damage in all tissues reached by the toxin and ultimately to death of the animal. PMID:5511567

  20. Bambuterol: uptake and metabolism in guinea pig isolated lungs

    International Nuclear Information System (INIS)

    Ryrfeldt, A.; Nilsson, E.; Tunek, A.; Svensson, L.A.

    1988-01-01

    The lung uptake and biotransformation of 3 H-bambuterol, a prodrug to terbutaline, were studied using isolated perfused and ventilated guinea pig lungs. 14 C-Sucrose was used as an extracellular marker. The lung uptake of bambuterol was significantly (0.05 greater than or equal to P greater than or equal to 0.001) higher than that found for sucrose in single-pass perfusion experiments. High-performance liquid chromatographic (HPLC) analysis showed that 95.6 +/- 3.6% of the effluent 3 H radioactivity was attributable to bambuterol. In recirculating experiments (120 min) the lung biotransformation of 3 H-bambuterol (8.5 pmol/ml) was studied. Both oxidative and hydrolytic metabolism took place. The dominating metabolites were hydroxylated bambuterol and the monocarbamate derivative which is a product of hydrolysis of bambuterol. Traces of terbutaline were also formed. The results show that bambuterol has a certain affinity to lung tissue and that the drug is, to some extent, biotransformed in the guinea pig lung

  1. The measurement of milk intake in the neonatal guinea pig

    International Nuclear Information System (INIS)

    Weaver, L.T.; Roberts, S.B.

    1990-01-01

    Study of the effects of enteral nutrition on neonatal growth and development has been hindered by lack of a suitable model. An isotope dilution method for the measurement of milk intake in suckling rodents was evaluated in 13 hand-fed neonatal guinea pigs, by comparing milk intakes determined using 3H20 with intakes determined by difference in weight before and after feeding. The effects of two experimental durations (1 or 2-3 days) on the accuracy and precision of the method was assessed. Weighted milk intakes were 18 -26 g/day, and body weight changes were -7% - +12% of initial weight. There was no significant difference between milk intakes determined using the two methods for both 1 and 2-3 day comparisons. However, the coefficients of variation for 1 and 2-3 day measurements were 24.4% and 5.5%, respectively. These values are equivalent to precisions of approximately 10% and 2% for estimates of total milk output in a rodent suckling 8 pups. It is concluded that the 3H20 method is no less accurate or precise than the weighing method for 2-3 day measurements of milk intake in individual pups and milk production of dams. These findings strengthen the use of the precocial guinea pig as a model for the study of the effects of early feeding on growth, development and aging

  2. Radiation effect on the middle ear of the guinea pig

    International Nuclear Information System (INIS)

    Harada, Hirofumi; Nishizawa, Shinji; Hiraide, Fumihisa; Inoue, Tetsuzo

    1984-01-01

    It is known that radiation therapy of the head and neck causes otitis media with effusion. Otitis media with effusion was induced in guinea pigs by cobalt-60 irradiation. Twenty guinea pigs with intact drum and normal Preyer reflex were used. We conducted, two experiments as follows: 1) The animals were irradiated with doses of 2,000 rad, 4,000 rad, and 6,000 rad. They were sacrificed seven days after the irradiation. 2) The animals were irradiated with doses of 4,000 rad and sacrificed one day, three days, seven days, and fourteen days after the irradiation. Vascular permeability of the middle ear mucosa was observed by Majno's vascular labelling technique. Pathological change of the middle ear was examined under the light microscope. Vascular permeability increased in three days after 4,000 rad irradiation and small vessels were labelled with carbon particles. Seven days after irradiation, carbon labelling of small vessels was more extensive and extravascular blackening was present in the adjacent tissues. Edematous change of the middle ear mucosa and metaplasia of the epithelial cells were also observed. New bone formation of the tympanic bulla was increased by repeated irradiations. (author)

  3. Spasmolytic effect of traditional herbal formulation on guinea pig ileum

    Directory of Open Access Journals (Sweden)

    Dushyant Kumar

    2015-01-01

    Full Text Available Background: The herbal formulation consisting of Andrographis paniculata Nees., Cassia fistula L., Foeniculum vulgare Mill. and Cuminum cyminum L. is widely used by the local traditional practitioners in rural Northern Karnataka for spasmodic abdominal pain. Objective: The present study was undertaken to evaluate safety and spasmolytic effect of poly-herbal formulation. Materials and Methods: Acute toxicity studies were carried out in Swiss mice, as per the Organization for Economic Co-operation and Development (OECD guidelines. The spasmolytic activity of the formulation was studied in isolated guinea pig ileum model using histamine and acetylcholine as agonists. The data were analyzed by one-way ANOVA, followed by Dunnetts post-hoc test and P ≤ 0.05 was considered as significant. Results: The formulation did not show any adverse toxic effects and found to be safe. It also showed significant (P < 0.05 relaxation in different agonist like histamine and acetylcholine-induced contractions in guinea pig ileum. Conclusions: Antispasmodic activity of the herbal formulation can be attributed to its atropine-like activity. The present findings, therefore, support its utility in spasmodic abdominal pain.

  4. Placental transfer of ruthenium in rat and guinea-pig

    International Nuclear Information System (INIS)

    Levack, V.M.; Pottinger, H.; Harrison, J.D.

    1994-01-01

    Ruthenium-106 in citrate solution was administered intravenously to rat at different stages of pregnancy and to guinea-pig either before conception or in late pregnancy. The results for rat showed that retention in the embryo/foetus measured at 3-5 days after administration increased from about 0.0002% of injected activity per embryo/foetus on day 12 of gestation to about 0.05% at birth. The relative concentrations of 106 Ru in embryo/foetus and mother (C f /C m ratio) were about 0.1 in each case. Concentrations in the yolk sac on day 12 were about 1% g -1 compared with 0.01% g -1 kin the foetus/ Retention in the guinea-pig foetus in late gestation at 7 days after administration (days 50-57) was about 0.2% injected activity per foetus, corresponding to a C f /C m = 0.2. Retention in each foetoplacental unit was 2% of injected 106 Ru with 50% in the yolk sac, 35% in the placenta and 10% in the foetus. For administration 4 weeks prior to conception, the level of 106 Ru retained in the foetus on day 57 of gestation was two orders of magnitude lower than after short-term administration, with a C f /C m about 0.004. (author)

  5. Effects of radiation on nasal mucosa of guinea pig

    International Nuclear Information System (INIS)

    Xiao Mang; Hu Sunhong; Liu Yuehui

    2004-01-01

    Objective: To study histopathological changes in nasal mucosa of guinea pigs following a series of irradiation. Methods: A total of 60 healthy guinea pigs were divided into two groups randomly: the irradiation group (n=30) and the control group (n=30). The animals of the irradiation group were exposed nasally to linear accelerator X-rays at 5.0 Gy once a week for three weeks. At 1 d, 1 w, 2 w, 4 w, 10 w and half year after X-irradiation five animals per one group were sacrificed randomly, at each time point. Their middle nasal turbinate mucosa was observed under optical microscope and electron microscope, and were analysed by image analysis for histopathological transformation. Results: In the irradiation group, the early histopathological transformation was acute inflammatory reaction, at the fourth week the mucosa began to repair, which ended at the sixth month. But after repaired, some parts of the repaired mucosa were lined with squamous epithelium instead of the normal nasal mucosa. The rate of the cilia-covered area was only 52.9% at the sixth month. Conclusion: The nasal mucosa injury and the squamous metaplasia could form a pathological basis of dysfunction after irradiation

  6. Review of gastric torsion in eight guinea pigs (Cavia porcellus).

    Science.gov (United States)

    Nógrádi, Anna Linda; Cope, Iain; Balogh, Márton; Gál, János

    2017-12-01

    The authors present eight cases of gastric dilatation and volvulus (GDV) in guinea pigs from the Department and Clinic of Exotic Animal and Wildlife Medicine, University of Veterinary Medicine, Budapest, Hungary between 2012 and 2016. Seven animals were operated on and two survived. Gastric torsion has been noted in many mammalian species. Gastric volvulus has a high morbidity and high mortality rate with a guarded to poor prognosis in all of these species. How GDV develops is still not widely understood. Postmortem examinations, in both our cases and previously reported cases, have failed to reveal the exact causes of the gastric torsions. The aetiology of gastric torsion in guinea pigs is probably multifactorial. Feeding fewer meals per day, eating rapidly, decreased food particle size, exercise, stress after a meal, competition, age, and an aggressive or fearful temperament, are all likely and potential risk factors for GDV development in a similar fashion to dogs. Sex, breeding, dental diseases, anatomical abnormalities, pain and pregnancy may also be contributing factors.

  7. Morphogenesis in guinea grass pastures under rotational grazing strategies

    Directory of Open Access Journals (Sweden)

    Denise Baptaglin Montagner

    2012-04-01

    Full Text Available This study was conducted in order to evaluate the morphogenetic and structural characteristics of guinea grass cv. Mombasa under three post-grazing heights (intense - 30 cm, lenient - 50 cm and variable - 50 in spring-summer and 30 cm in autumn-winter when sward light interception reached 95% during regrowth. Post-grazing heights were allocated to experimental units (0.25 ha in a completely randomized block design with three replications. Post-grazing heights affected only leaf elongation rate and the number of live leaves. Pastures managed with variable post-grazing height showed higher leaf elongation rate in the summer of 2007. This management strategy also resulted in a higher number of live leaves. During the spring of 2006, plants showed lower leaf elongation rate, leaf appearance rate and number of live leaves, and greater phyllochron and leaf lifespan. In contrast, during the summer of 2007, the leaf appearance rate, leaf elongation rate, number of live leaves, and final leaf length were greater while phyllochron, stem elongation rate, and leaf senescence rate were lower. The management of the guinea grass cv. Mombasa with intense or variable post-grazing height throughout the year seems to represent an interesting management target, in terms of leaf appearance rate and number of live leaves.

  8. La evolución del piso subalpino en la Sierra de Urbión (Sistema Ibérico, norte de españa: un modelo de impacto geoecológico de actividades humanas en el Valle de Ormazal

    Directory of Open Access Journals (Sweden)

    García-Ruiz, José M.

    2016-12-01

    Full Text Available This paper is a synthesis of the landscape, geomorphic and functional evolution of the subalpine belt in the Urbión Sierra, providing a geoecological perspective of the interactions between human activity, spatial organization, geomorphological dynamics and recent land use changes. Landscape changes in the subalpine belt started at least during the Late Neolithic, with forest fires that tried to waste the forest to enable the expansion of summer grasslands favouring an incipient sheep transhumance. Fires occurred also through the Chalcolithic and the Bronze and Iron Ages, and culminated during the Middle Ages. Deforestation of the subalpine belt would be responsible for the triggering of a number of shallow landslides and soil erosion in steep slopes above 1500 m a.s.l. The crisis of the transhumance since the beginning of the 19th century reduced the livestock pressure, particularly in the second half of the 20th century, and has contributed to shrub and forest expansion, whereas the area occupied by summer grasslands has been remarkably reduced. The decreasing livestock pressure suggests that forest expansion will continue in the next future, in a favourable context of global warming and declining presence of snowpack in the subalpine belt.Este trabajo es una síntesis de la evolución paisajística, funcional y geomorfológica del piso subalpino de la Sierra de Urbión, proporcionando una perspectiva geoecológica de las interacciones entre actividades humanas, organización del espacio, dinámica geomorfológica y transformaciones recientes en los usos del suelo. Los cambios paisajísticos del piso subalpino en la Sierra de Urbión se iniciaron al menos en el Neolítico tardío con incendios que procuraban eliminar el bosque para permitir la expansión de los pastos de aprovechamiento estival y así favorecer una incipiente trashumancia de ganado lanar. Los incendios se prolongaron a lo largo del Calcolítico y las edades del Bronce y del

  9. Pathogenesis of a Chinese strain of bovine adenovirus type 3 infection in albino guinea pigs.

    Science.gov (United States)

    Shi, Hong-Fei; Zhu, Yuan-Mao; Yan, Hao; Ma, Lei; Wang, Xue-Zhi; Xue, Fei

    2014-12-01

    Bovine adenovirus type 3 (BAV-3) is considered one of the most important respiratory tract agents of cattle and is widespread among cattle around the world. A BAV-3 strain was isolated from a bovine nasal swab for the first time in China in 2009 and named HLJ0955. Subsequently, BAV-3 has frequently been isolated from calves with respiratory diseases in China. To date, only limited study on the pathogenesis of BAV-3 infection in cotton rats has been conducted, and the pathogenesis of BAV-3 infection in guinea pigs has not been reported. Therefore, sixteen albino guinea pigs were inoculated intranasally with HLJ0955. All of the infected guinea pigs had apparently elevated rectal temperatures (39.2 °C-39.9 °C) at 2-7 days post-inoculation (PI). Consolidation and petechial hemorrhage were also observed in guinea pigs experimentally infected with HLJ0955. Viral replication was detectable by virus isolation and titration and by immunohistochemistry in the lungs of guinea pigs as early as 24 h PI. Viral DNA was detectable in the lungs of infected guinea pigs during 11 days of observation by real-time PCR. Virus-neutralizing antibodies against BAV-3 were detectable from 11 days PI and reached a peak titer at 15 days PI. Histopathological changes mainly occurred in the lungs of infected guinea pigs and were characterized by thickening of alveolar septa, mononuclear cell infiltration, hemorrhage and alveolar epithelial necrosis. These results indicate that HLJ0955 can replicate in the lungs of guinea pigs and cause fever and gross and histological lesions. The guinea pig infection model of BAV-3 would serve as a useful system for monitoring the infection process and pathogenesis of the Chinese BAV-3 strain HLJ0955, as well as immune responses to BAV-3 vaccines.

  10. Natural infection of guinea pigs exposed to patients with highly drug-resistant tuberculosis

    Science.gov (United States)

    Dharmadhikari, Ashwin S.; Basaraba, Randall J.; Van Der Walt, Martie L.; Weyer, Karin; Mphahlele, Matsie; Venter, Kobus; Jensen, Paul A.; First, Melvin W.; Parsons, Sydney; McMurray, David N.; Orme, Ian M.; Nardell, Edward A.

    2012-01-01

    A natural TB infection model using guinea pigs may provide useful information for investigating differences in transmission efficiency and establishment of active disease by clinical TB strains in a highly susceptible host under controlled environmental conditions. We sought to examine the capacity of naturally transmitted multidrug-resistant M. tuberculosis to establish infection and produce active disease in guinea pigs. Guinea pigs were continuously exposed for 4 months to the exhaust air of a 6-bed multidrug-resistant tuberculosis inpatient hospital ward in South Africa. Serial tuberculin skin test reactions were measured to determine infection. All animals were subsequently evaluated for histologic disease progression at necropsy. Although 75% of the 362 exposed guinea pigs had positive skin test reactions [≥6mm], only 12% had histopathologic evidence of active disease. Reversions (≥ 6 mm change) in skin test reactivity were seen in 22% of animals, exclusively among those with reactions of 6 to 13 mm. Only two of 86 guinea pigs with reversion had histological evidence of disease compared to 47% (31/66) of guinea pigs with large, non-reverting reactions. Immunosuppression of half the guinea pigs across all skin test categories did not significantly accelerate disease progression. In guinea pigs that reverted a skin test, a second positive reaction in 27 (33%) of them strongly suggested re-infection due to ongoing exposure. These results show that a large majority of guinea pigs naturally exposed to human-source strains of multidrug-resistant tuberculosis became infected, but that many resolved their infection and a large majority failed to progress to detectable disease. PMID:21478054

  11. Towards global Guinea worm eradication in 2015: the experience of South Sudan.

    Science.gov (United States)

    Awofeso, Niyi

    2013-08-01

    For centuries, the Guinea worm parasite (Dracunculus medinensis) has caused disabling misery, infecting people who drink stagnant water contaminated with the worm's larvae. In 2012, there were 542 cases of Guinea worm reported globally, of which 521 (96.1%) were reported in South Sudan. Protracted civil wars, an inadequate workforce, neglect of potable water provision programs, suboptimal Guinea worm surveillance and case containment, and fragmented health systems account for many of the structural and operational factors encumbering South Sudan's Guinea worm eradication efforts. This article reviews the impacts of six established Guinea worm control strategies in South Sudan: (1) surveillance to determine actual caseload distribution and trends in response to control measures; (2) educating community members from whom worms are emerging to avoid immersing affected parts in sources of drinking water; (3) filtering potentially contaminated drinking water using cloth filters or filtered drinking straws; (4) treating potentially contaminated surface water with the copepod larvicide temephos (Abate); (5) providing safe drinking water from boreholes or hand-dug wells; and (6) containment of transmission through voluntary isolation of each patient to prevent contamination of drinking water sources, provision of first aid, and manual extraction of the worm. Surveillance, community education, potable water provision, and case containment remain weak facets of the program. Abate pesticide is not a viable option for Guinea worm control in South Sudan. In light of current case detection and containment trends, as well as capacity building efforts for Guinea worm eradication, South Sudan is more likely to eradicate Guinea worm by 2020, rather than by 2015. The author highlights areas in which substantial improvements are required in South Sudan's Guinea worm eradication program, and suggests improvement strategies. Copyright © 2013 International Society for Infectious

  12. Efeito da suplementação protéica a pasto sobre consumo de forragens, ganho de peso e condição corporal, em vacas Nelore

    Directory of Open Access Journals (Sweden)

    Ruas José Reinaldo Mendes

    2000-01-01

    Full Text Available O objetivo do presente trabalho foi verificar o efeito da utilização de concentrado protéico, durante a época de verão, sobre consumo de forragem e variação no ganho de peso e no escore da condição corporal após o parto. Foram utilizadas 51 vacas paridas da raça Nelore, distribuídas ao acaso nos seguintes tratamentos: T0 = sem suplementação; T1 = suplementação com 1 kg; e T2 = suplementação com 2 kg de concentrado contendo 40,8% de PB, durante 105 dias. Neste período, foram determinados o escore corporal e o peso. Para determinar o consumo, utilizaram-se o marcador externo óxido de cromo, em dose única diária de 20 g, e o marcador interno FDA indigestível das extrusas, coletadas por meio de fístulas esofágicas. A suplementação não influenciou o consumo de MS de pasto, que ficou em torno de 9,87 kg MS/dia, correspondente a 2,13% do peso vivo, mas o consumo total de MS foi superior nos tratamentos T1 e T2. Escore da condição corporal, ganho de peso diário e peso final das vacas mostraram-se superiores nos animais suplementados em relação aos não-suplementados. Não foi observada diferença entre os pesos das crias ao final do período experimental.

  13. On the western fringe of baboon distribution: mitochondrial D-loop diversity of Guinea Baboons (Papio papio Desmarest, 1820 (Primates: Cercopithecidae in Coastal Guinea-Bissau, western Africa

    Directory of Open Access Journals (Sweden)

    M.J. Ferreira da Silva

    2013-06-01

    Full Text Available Like many primate species in West Africa, habitat loss and intensive hunting are threatening the poorly studied Guinea Baboon (Papio papio. These factors contributed to a significant population contraction during the last 30 years. Our study presents genetic diversity estimates for the Guinea Baboon based on a 391 base pair fragment of the mitochondrial DNA D-loop hypervariable region I. We used non-invasively collected genetic samples from two locations in Guinea-Bissau: Cufada Lagoons Natural Park and Cantanhez Forest National Park. Although most sampling was opportunistic, we observed and collected samples from two dames (social units. Among the 25 sequences obtained, we found seven closely related mtDNA haplotypes and one highly different haplotype. The presence of this divergent haplotype suggests a contact area between genetically differentiated populations in Cufada Lagoons Natural Park, or dispersal of individuals. The samples gathered from both regions share two of the most common haplotypes in different frequencies, but also exhibit unique haplotypes. No significant genetic differentiation was found between social units from both regions, possibly due to common ancestral origin or frequent dispersal between sampling locations. The presence of different maternal lineages in the same social unit and a higher percentage of variation within social units suggest historical female-biased dispersal for Guinea-Bissau Baboons. We further compared mitochondrial genetic diversity of Guinea and Hamadryas Baboons. We found lower haplotype, nucleotide and theta diversity for Guinea Baboons, which points to different demographic histories of these species. This work supports the need for additional genetic studies within the full Guinea Baboon range.

  14. Detección De Patrones De Deserción En Los Programas De Pregrado De La Universidad Mariana De San Juan De Pasto, Aplicando El Proceso De Descubrimiento De Conocimiento Sobre Base De Datos (Kdd) Y Su Implementación En Modelos MatemáTicos De Predicción

    OpenAIRE

    Argote, Ivan; Jimenez, Robinson

    2016-01-01

    La investigación titulada: “Detección de Patrones de Deserción en los Programas de Pregrado de la Universidad Mariana de San Juan de Pasto, aplicando el proceso de descubrimiento de conocimiento sobre base de datos (KDD) y su implementación en modelos matemáticos de predicción”. Es un proyecto que tiene como objetivo primordial Aportar al Proceso de Toma de Decisiones para disminuir los niveles Deserción Estudiantil en los programas de pregrado de la Universidad Mariana, mediante el descubrim...

  15. Treatment and control of Trixacarus caviae infestation in a conventional guinea pig (Cavia porcellus) breeding colony

    OpenAIRE

    Nath, Anjan Jyoti

    2015-01-01

    A case of sarcoptic mange caused by Trixacarus caviae in a conventional guinea pig breeding colony is reported. The infestation was reported in a large colony of guinea pigs during the month of July, 2013 affecting 30 breeder guinea pigs. Severely infested animals were treated individually with subcutaneous injection of ivermectin 1 % w/v (Neomec®) at the rate of 400 µg/kg body weight 10 days apart. Three doses of ivermectin were sufficient to eliminate the parasite which tested negative afte...

  16. Transmission pattern of parainfluenza 3 virus in guinea pig breeding herds.

    Science.gov (United States)

    Blomqvist, Gunilla A M; Martin, Krister; Morein, Bror

    2002-07-01

    In searching for the cause of experimental variations in respiratory research data, serology revealed the prevalence of antibodies against parainfluenza virus type 3 (PIV 3) in guinea pigs. The aim of the present study was to explore the transmission rate, course, and kinetics of enzootic PIV 3 infection in guinea pig breeding units. In the first part of the study, blood samples to be analyzed for PIV 3 antibodies were collected from guinea pigs of a PIV 3-positive breeding colony at different times after birth. In the same breeding unit, 6 of 12 2-week-old guinea pigs were relocated and separately housed. The PIV 3 serum antibody titers of the two groups were compared at various times from birth to 13 weeks after birth. In the second part of the study, the spread of infectious virus and virus persistence were explored by housing seronegative sentinel animals together with 2- to 3-week-old guinea pigs from three different PIV 3-positive breeding units. The guinea pigs remaining in the breeding colony as well as those removed and housed separately showed declining serum antibody titers for about 1 month after birth, thereafter the titers were stable until about 8 weeks after birth. Five weeks later, the mean antibody titer of the guinea pigs remaining in the breeding colony had increased to a markedly higher level than that of the relocated, separately housed guinea pigs. Seroconversion was demonstrated in 7 of the 14 sentinels housed with the 2- to 3-week-old guinea pigs from PIV 3-positive breeding units. Sentinels housed together with PIV 3-positive guinea pigs 24 weeks after the start of the experiment did not seroconvert. We conclude that young guinea pigs born to PIV 3-positive mothers were protected by maternal immunity against infection with PIV 3 during their first 14 days of life. The guinea pig offspring became infected during the period from about 2 weeks until 8 weeks after birth, as demonstrated by seroconversion of sentinel animals and an increasing

  17. Lower Cretaceous Source Rock and its Implication for the Gulf of Guinea Petroleum System

    International Nuclear Information System (INIS)

    Frost, B.R.; Griffith, R.C.

    2002-01-01

    Current petroleum system models for the Gulf of Guinea propose Tertiary-age deltaic organic material as the principal source for the hydrocarbons found there. Although previous workers recognized numerous difficulties and inconsistencies, no alternative model has been resented to adequately explain the complete petroleum system. We propose that the principal source rock for the Gulf of Guinea system occurs in upper lower Cretaceous-age shales at the rift-drift transition. Tertiary loading and the consequent maturation of this lower Cretaceous source rock can explain the controls on tap formation, reservoir distribution and hydrocarbon types found in the Gulf of Guinea

  18. Acute Inhalation Toxicity of T-2 Mycotoxin in the Rat and Guinea Pig

    Science.gov (United States)

    1990-01-01

    2/kg body weight for the guinea pig . These data show that inhaled T-2 toxin is approximately 20 times more toxic to the rat (0.05 mg T-2/kg body wt...inhaled vs 1.0 mg T-2/kg body wt ip) and at least twice as toxic to the guinea pig (0.4 mg T-2/kg body wt inhaled vs 1-2 mg T-2/kg body wt ip) than ip...administered T-2 toxin. Histopathologic examination of major organs in both the rat and guinea pig after respiratory exposure to T-2 toxin indicated

  19. New Methods for Evaluating Skin Injury from Sulfur Mustard in the Hairless Guinea Pig

    Science.gov (United States)

    1993-05-13

    MUSTARD IN THE HAIRLESS GUINEA PIG Ernest H. Braue, Jr., Catherine R. Bangledorf, and Robert G. Rieder "U.S. Army Medical Research Institute of Chemical...evaluating the skin hydration state. The skin of anesthetized hairless guinea pigs was exposed to saturated HD vapor (1.4mg/ml) at 4 sites for 3, 5, 7, or 9...assessment of skin damage following cutaneous exposure to HD vapor. EXPERIMENTAL METHODS Each hairless guinea pig (HGP) was exposed to saturated HD vapor

  20. Gamma Radiation (5-10 Gy) Impairs Neuronal Function in the Guinea Pig Hippocampus

    Science.gov (United States)

    1993-01-01

    Radiation (5-10 Gy) Impairs Neuronal Function in the Guinea Pig Hippocampus TERRY C. PELLMAR AND DENNIS L. LEPINSKI Ph.vsiology Department..Irmned Forces...L. Gamma Radiation ioral effects. Within hours of irradiation with 10 Gy and (5- 10 Gy) Impairs Neuronal Function in the Guinea Pig Hippo- less...acti v- Guinea pigs were exposed to 5 and 10 Gy ’y radiation. Hippo- ity (9) are evident. campal brain slices were isolated 30 min, I day, 3 days and 5

  1. Experimental Research Into High Barometric Oxygen Prevention of Guinea Pig Hearing Loss,

    Science.gov (United States)

    1992-08-28

    PREVENTION OF GUINEA PIG HEARING LOSS by Yin Jiacai, Sun Fang ren, et al. DTIC MLECTE •<• EP 2 9 1992 Approved for public release, Distribution unlimited...PREVENTION OF GUINEA PIG HEARING LOSS By: Yin Jiacai, Sun Fang ren, et al. English pages: 9 Source: Chung-Hua I Shueh Tsa Chih, Vol. 65, Nr. 11, Nov.eember...Distributionf._DL~~~t .•b • / or __ Dlist szeccat .lef ’ ~1 EXPERIMENTAL RESEARCH INTO HIGH BAROMETRIC OXYGEN PREVENTION OF GUINEA PIG HEARING LOSS BY: Yin

  2. A reliable method for reconstituting thymectomized, lethally irradiated guinea pigs with bone marrow cells

    International Nuclear Information System (INIS)

    Terata, N.; Tanio, Y.; Zbar, B.

    1984-01-01

    The authors developed a reliable method for reconstituting thymectomized, lethally irradiated guinea pigs. Injection of 2.5-10 x 10 7 syngeneic bone marrow cells into adult thymectomized, lethally irradiated guinea pigs produced survival of 46-100% of treated animals. Gentamycin sulfate (5 mg/kg of body weight) for 10 days was required for optimal results. Acidified drinking water (pH 2.5) appeared to be required for optimal results. Thymectomized, lethally irradiated, bone marrow reconstituted ('B') guinea pigs had impaired ability to develop delayed cutaneous hypersensitivity to mycobacterial antigens and cutaneous basophil hypersensitivity to keyhole limpet hemocyanin; proliferative responses to phytohemagglutinin were impaired. (Auth.)

  3. Dinámica del cadmio en suelos cultivados con papa en Nariño, Colombia. Dinamic of the cadmium in soils cultivated with potatoes in Nariño, Colombia

    Directory of Open Access Journals (Sweden)

    Liliana Insuasty B

    2008-01-01

    Full Text Available Se evaluó la fijación y movilidad de cadmio en suelos dedicados al cultivo de papa en Túquerres, Pasto y Guachucal. Se tomaron muestras de suelos no disturbadas en cilindros de PVC, las cuales, luego de aplicaciones fraccionadas de cadmio con dosis de 0, 50,100 y 150 ppm, se incubaron a capacidad de campo durante cuatro meses. En estos suelos los contenidos de cadmio total e intercambiable no sobrepasaron los límites permisibles establecidos por Organización Mundial de la Salud-Organización de las Naciones Unidas para la Agricultura y la Alimentación (OMS-FAO; no obstante, la mayor cantidad del elemento se localizó en la parte superior de los núcleos, lo cual indica baja movilidad del elemento en el perfil.The mobility of cadmium and fixation in soils cropped with potatoes in Túquerres, Pasto and Guachucal Nariño, Colombia was evaluated. Samples with an auger of 11 cm diameter and 40 cm in length were taken. On these soil samples applications of 0, 50, 100 and 150 ppm cadmium were, and incubated during a 4 month period under field capacity. In this soils the content of total exchangeable cadmium do not overcome the permissible limits established by World Health Organization-Food and Agriculture Organization of the United Nations. The highest concentration of cadmium applied in the for of total Cd and changeable Cd was found in the higher third part of cores, which indicates a low mobility of the element in the soil profile.

  4. Dinamic of the cadmium in soils cultivated with potatoes in Nariño, Colombia Dinámica del cadmio en suelos cultivados con papa en Nariño, Colombia.

    Directory of Open Access Journals (Sweden)

    Burbano Orjuela Hernán

    2008-03-01

    Full Text Available The mobility of cadmium and fixation in soils cropped with potatoes in Túquerres, Pasto and Guachucal Nariño, Colombia was evaluated. Samples with an auger of 11 cm diameter and 40 cm in length were taken. On these soil samples applications of 0, 50, 100 and 150 ppm cadmium were, and incubated during a 4 month period under field capacity. In this soils the content of total exchangeable cadmium do not overcome the permissible limits established by World Health Organization–Food and Agriculture Organization of the United Nations. The highest concentration of cadmium applied in the for of total Cd and changeable Cd was found in the higher third part of cores, which indicates a low mobility of the element in the soil profile.Se evaluó la fijación y movilidad de cadmio en suelos dedicados al cultivo de papa en Túquerres, Pasto y Guachucal. Se tomaron muestras de suelos no disturbadas en cilindros de PVC, las cuales, luego de aplicaciones fraccionadas de cadmio con dosis de 0, 50,100 y 150 ppm, se incubaron a capacidad de campo durante cuatro meses. En estos suelos los contenidos de cadmio total e intercambiable no sobrepasaron los límites permisibles establecidos por Organización Mundial de la Salud–Organización de las Naciones Unidas para la Agricultura y la Alimentación (OMS–FAO; no obstante, la mayor cantidad del elemento se localizó en la parte superior de los núcleos, lo cual indica baja movilidad del elemento en el perfil.

  5. Il dono del racconto

    DEFF Research Database (Denmark)

    Polito, Paola

    1998-01-01

    L'articolo indaga a vari livelli strutturali del romanzo "Il coraggio del pettirosso" (rete metaforica, dialogismo diegetico, dialogismo come fenomeno di voce, strategie discorsive messe in atto dalla voce narrante, stile colloquiale) la complessa realizzazione testuale del genere narrativo...

  6. Bartonellosis en Colombia, bartonellosis de guáitara ó fiebre verrucosa del guáitara

    Directory of Open Access Journals (Sweden)

    Luis Patiño Camargo

    1939-04-01

    Full Text Available Desde principios de 1936 las autoridades de Nariño venían seriamente preocupadas por una mortífera enfermedad epidémica aparecida al Norte en vertientes de juanambu y del rio Mayo, enfermedad que de improviso se presentó en el Guáitara con gran virulencia. El médico doctor Max Llorente, Gobernador entonces del Departamento, inspeccionó con el Director de Higiene la zona invadida, y a su regreso a Pasto conferencio con el Colegio Médico. En la reunión se acogió el diagnostico de paludismo agudo pernicioso. Como resultado se organizó una comisión de tratamiento encabezada por un médico, y se situó en Ancuya. Por esos días el Departamento Nacional de Higiene envió un ingeniero sanitario, quien recorrió las comarcas atacadas y rindió un informe.

  7. Aristotle, alive and well in Papua New Guinea science classrooms

    Science.gov (United States)

    Boeha, Beno B.

    1990-09-01

    National High School students from Papua New Guinea were interviewed about two situations; the results of their Aristotle-like views regarding `forced' and `natural' motion are presented and discussed. Twenty-one National High School Students were interviewed about two situations similarly used elsewhere (Osborne and Gibert 1979, 1980, Osborne 1980a) and the results of these Aristotle-like views possessed by students have been presented and discussed above. With each of the six summary statements some extracts have been provided from interviews conducted by the author with students who had come from various parts of Pupua New Guinea. Students' views have been compiled to give a composite picture of the Aristotle-like ideas. Some impression of the commonality of the ideas/beliefs has been provided by reference to the work of others who have reported similar tendencies in testing and interviewing physics students. Throughout the study, students' Aristotlean-like views have been given a respected status that reflects their widespread use, their internal coherence and their tenacity in the face of classroom teaching in a Pupua New Guinea National High School. In analysing individual interview transcripits, attempts were made to construct ideas/beliefs that can account for statements by each student in a manner that statements are consistent with each other. The assumption that all of a student's statements are logically compatible to a listener or reader is difficult to maintain. However, it is one that has to be made as a hypothesis to work with, otherwise it is too easy to discount sections of a student's discourse that seem inconsistent with understandable parts. The aims of this part of the study have been to provide science educationalists with a repertoire of common Aristolean-like beliefs which have persisted in students. These views differ in some ways from the orthodox physics views. By better understanding of the students' beliefs and commitments about

  8. Leukotriene B4 receptors on guinea pig alveolar eosinophils

    International Nuclear Information System (INIS)

    Maghni, K.; de Brum-Fernandes, A.J.; Foeldes-Filep, E.G.; Gaudry, M.; Borgeat, P.; Sirois, P.

    1991-01-01

    The existence of receptors for LTB4 on highly purified guinea pig alveolar eosinophils was investigated. Massive infiltration of eosinophils in alveolar spaces was induced in guinea pigs by i.v. injections of Sephadex beads G50 (16 mg/kg). Alveolar eosinophils (50 x 10(6) cells) were purified to approximately 98% by Percoll continuous density gradient centrifugation. The binding studies indicated that alveolar eosinophils bind LTB4 in a saturable, reversible and specific manner. Scatchard analysis indicated the existence of high-affinity binding sites (Kd1 = 1.00 ± 0.22 nM; Bmax1 = 966 ± 266 sites/cell) and low-affinity binding sites (Kd2 = 62.5 ± 8.9 nM; Bmax2 = 5557 ± 757 sites/cell). The metabolism of LTB4 by alveolar eosinophils in binding conditions was assessed by RP-HPLC and no significant degradation of [3H]LTB4 was observed. LTB4 dose-dependently stimulated eosinophil migration in both chemokinesis and chemotaxis assays with an EC50 value of 1.30 ± 0.14 and 18.14 ± 1.57 nM, respectively. LTB4 caused a dose-dependent increase in the production of superoxide anion with an apparent EC50 value of 50 x 10(-9) M in the authors experimental conditions. LTB4 also induced a dose-dependent increase in the generation of TxA2 with an EC50 value of 46.2 x 10(-9) M. Taken together, their results demonstrated that guinea pig alveolar eosinophils express two classes of specific receptors for LTB4. The high-affinity binding sites seem associated to chemokinesis and chemotaxis whereas the low-affinity binding sites seem associated to superoxide anion production and generation of TxA2. The existence of LTB4 receptors in eosinophils could explain the presence of these cells in hypersensitivity reactions

  9. Estructura espacial de Eucinostomus argenteus (Pisces: Gerreidae en la zona norte del Caribe colombiano

    Directory of Open Access Journals (Sweden)

    Jorge Paramo

    2010-01-01

    Full Text Available Esta investigación se llevó a cabo para determinar la distribución espacial de la densidad (captura por unidad de área – CPUA y de la estructura de tallas de Eucinostomus argenteus y su relación con las variables ambientales en la zona norte del Caribe colombiano. Los datos fueron se tomaron durante un crucero de investigación realizado en  la época seca (febrero de 2006 entre  Puerto Estrella (La Guajira y Santa Marta (Magdalena. Las muestras biológicas se colectaron con una red de arrastre (en estratos de profundidad < 50 m y 50-100 m siguiendo un diseño de muestreo sistemático. Los individuos maduros y de tallas mayores se encontraron distribuidos principalmente entre Manaure y Punta Gallinas, donde la plataforma continental es muy ancha y la oceanografía local esta modulada por la surgencia estacional. Los peces juveniles, se distribuyeron al sur del área de estudio, entre Boca Camarones y el Río Buritaca, cerca de la costa. En este sector la plataforma es muy estrecha y con alta productividad biológica por la presencia de ríos, manglares y pastos marinos, sirviendo como zona de alimentación y refugio para E. argenteus. En general los resultados sugieren que las variables ambientales son importantes para la distribución espacial de la abundancia y las tallas de  E. argenteus en la zona norte del Caribe colombiano, siendo la temperatura y la profundidad las variables que predijeron mejor la distribución espacial de la especie.

  10. Las Cucharas y Leticia: dos sitios arqueológicos tardíos en el Canal del Dique. Avance de investigación

    Directory of Open Access Journals (Sweden)

    Diana Rocío Carvajal Contreras

    2013-01-01

    Full Text Available La información de este artículo provino del proyecto de investigación en curso “Evaluación Zooarqueológica de concheros cercanos al Canal del Dique, Fase Inicial”. Se procedió a la ubicación de los puntos identificados por Macchi en 1998, registrando dos sitios con materiales posteriores al Formativo: las Cucharas y Leticia. Las evidencias se asociaron a fechas entre los siglos XIII a XIX. Uno de ellos tal vez esté relacionado con las actividades de reapertura del Canal bajo la dirección de G.M. Totten. Por otro lado, los taxones de moluscos indicaron que las personas que habitaban en los sitios arqueológicos, principalmente Leticia, se desenvolvían en un ambiente costero con diversos hábitats de playa arenosas, playas fangosas, manglar, zonas rocosas y de alta energía, zonas intermareales o hábitats de arena-cascajo, fondo mixto, pradera de pastos, y pradera de macroalga.

  11. Epidemiology of Epidemic Ebola Virus Disease in Conakry and Surrounding Prefectures, Guinea, 2014-2015.

    Science.gov (United States)

    Rico, Adriana; Brody, Debra; Coronado, Fátima; Rondy, Marc; Fiebig, Lena; Carcelen, Andrea; Deyde, Varough M; Mesfin, Samuel; Retzer, Kyla D; Bilivogui, Pepe; Keita, Sakoba; Dahl, Benjamin A

    2016-02-01

    In 2014, Ebola virus disease (EVD) in West Africa was first reported during March in 3 southeastern prefectures in Guinea; from there, the disease rapidly spread across West Africa. We describe the epidemiology of EVD cases reported in Guinea's capital, Conakry, and 4 surrounding prefectures (Coyah, Dubreka, Forecariah, and Kindia), encompassing a full year of the epidemic. A total of 1,355 EVD cases, representing ≈40% of cases reported in Guinea, originated from these areas. Overall, Forecariah had the highest cumulative incidence (4× higher than that in Conakry). Case-fatality percentage ranged from 40% in Conakry to 60% in Kindia. Cumulative incidence was slightly higher among male than female residents, although incidences by prefecture and commune differed by sex. Over the course of the year, Conakry and neighboring prefectures became the EVD epicenter in Guinea.

  12. The effect of restraining on the heart rate in guinea pigs

    Science.gov (United States)

    Mikiskova, H.

    1980-01-01

    The emotional effect of different applications of electrodes and the fixation for cariographic examination was investigated using guinea pigs. The effect of the stress is discussed in terms of heart rhythm and behavior.

  13. Practice and problems in radiation protection in medical institutions in Papua New Guinea

    International Nuclear Information System (INIS)

    Patel, I.C.

    1984-01-01

    Sources of ionizing radiations employed in medical centres in Papua New Guinea are outlined and the present practice in radiation protection is discussed. Steps being taken or proposed to improve the standard of radiological protection are also considered

  14. Ecological data in support of an analysis of Guinea-Bissau׳s medicinal flora

    Directory of Open Access Journals (Sweden)

    Luís Catarino

    2016-06-01

    Full Text Available This dataset presents an annotated list of medicinal plants used by local communities in Guinea-Bissau (West Africa, in a total of 218 species. Data was gathered by means of herbarium and bibliographic research, as well as fieldwork. Biological and ecological information is provided for each species, including in-country distribution, geographical range, growth form and main vegetation types. The dataset was used to prepare a paper on the medicinal plants of Guinea-Bissau “Medicinal plants of Guinea-Bissau: therapeutic applications, ethnic diversity and knowledge transfer” (Catarino et al., 2016 [1]. The table and figures provide a unique database for Guinea-Bissau in support of ethno-medical and ethno-pharmacological research, and their ecological dimensions.

  15. A guinea pig model of acute and chronic asthma using permanently instrumented and unrestrained animals

    NARCIS (Netherlands)

    Meurs, Herman; Santing, Ruud E.; Remie, Rene; van der Mark, Thomas W.; Westerhof, Fiona J.; Zuidhof, Annet B.; Bos, I. Sophie T.; Zaagsma, Johan

    2006-01-01

    To investigate mechanisms underlying allergen-induced asthmatic reactions, airway hyperresponsiveness and remodeling, we have developed a guinea pig model of acute and chronic asthma using unanesthetized, unrestrained animals. To measure airway function, ovalbumin (IgE)-sensitized animals are

  16. Family Outbreaks of Nontyphoidal Salmonellosis following a Meal of Guinea Pigs

    Directory of Open Access Journals (Sweden)

    John B. Fournier

    2015-01-01

    Full Text Available Salmonella outbreaks have been linked to a wide variety of foods, including recent nationwide outbreaks. Guinea pig (Cavia porcellus, also known as cuy or cobayo, has long been a popular delicacy and ceremonial food in the Andean region in South America. This case report describes three family outbreaks of nontyphoidal salmonellosis, each occurring after a meal of guinea pigs. We believe this case report is the first to describe a probable association between the consumption of guinea pig meat and human salmonellosis. Physicians should be aware of the association of Salmonella and the consumption of guinea pigs, given the increasing immigration of people from the Andean region of South America and the increasing travel to this region.

  17. Family Outbreaks of Nontyphoidal Salmonellosis following a Meal of Guinea Pigs.

    Science.gov (United States)

    Fournier, John B; Knox, Kimberly; Harris, Maureen; Newstein, Michael

    2015-01-01

    Salmonella outbreaks have been linked to a wide variety of foods, including recent nationwide outbreaks. Guinea pig (Cavia porcellus), also known as cuy or cobayo, has long been a popular delicacy and ceremonial food in the Andean region in South America. This case report describes three family outbreaks of nontyphoidal salmonellosis, each occurring after a meal of guinea pigs. We believe this case report is the first to describe a probable association between the consumption of guinea pig meat and human salmonellosis. Physicians should be aware of the association of Salmonella and the consumption of guinea pigs, given the increasing immigration of people from the Andean region of South America and the increasing travel to this region.

  18. SULFAMETHOXAZOLE-TRIMETHOPRIM TREATMENT OF GUINEA PIGS INFECTED WITH 'LEGIONELLA PNEUMPOPHILA'

    Science.gov (United States)

    Legionnaires' disease is a bacterial pneumonia caused by Legionella pneumophila. Many antibiotics inhibit the growth of L. pneumophila in vitro, but only erythromycin and rifampin have been clinically effective. Parallel results have been observed in guinea pigs infected ip with ...

  19. COMPARATIVE GENOTOXIC RESPONSES TO ARSENITE IN GUINEA PIG, MOUSE, RAT AND HUMAN LYMPHOCYTES

    Science.gov (United States)

    Comparative genotoxic responses to arsenite in guinea pig, mouse, rat and human lymphocytes.Inorganic arsenic is a known human carcinogen causing skin, lung, and bladder cancer following chronic exposures. Yet, long-term laboratory animal carcinogenicity studies have ...

  20. Pharmacokinetic Studies of Intramuscular Midazolam in Guinea Pigs Challenged With Soman

    National Research Council Canada - National Science Library

    Capacio, Benedict R; Byers, C. E; Merk, K. A; Smith, J. R; McDonough, J. H

    2004-01-01

    ...) activity following intramuscular (im) injection to soman-exposed guinea pigs (Crl:(HA)BR). Prior to experiments, the animals were surgically implanted with EEG leads to monitor seizure activity...

  1. In vivo effects of the IKr agonist NS3623 on cardiac electrophysiology of the guinea pig

    DEFF Research Database (Denmark)

    Hansen, Rie Schultz; Olesen, Søren-Peter; Rønn, Lars Christian B

    2008-01-01

    to examining the in vivo effects of NS3623. Injection of 30 mg/kg NS3623 shortened the corrected QT interval by 25 +/- 4% in anaesthetized guinea pigs. Accordingly, 50 mg/kg of NS3623 shortened the QT interval by 30 +/- 6% in conscious guinea pigs. Finally, pharmacologically induced QT prolongation by a h......ERG channel antagonist (0.15 mg/kg E-4031) could be reverted by injection of NS3623 (50 mg/kg) in conscious guinea pigs. In conclusion, the present in vivo study demonstrates that injection of the hERG channel agonist NS3623 results in shortening of the QTc interval as well as reversal of a pharmacologically...... induced QT prolongation in both anaesthetized and conscious guinea pigs....

  2. Type I hair cell degeneration in the utricular macula of the waltzing guinea pig

    DEFF Research Database (Denmark)

    Severinsen, Stig A; Raarup, Merete Krog; Ulfendahl, Mats

    2008-01-01

    Waltzing guinea pigs are an inbred guinea pig strain with a congenital and progressive balance and hearing disorder. A unique rod-shaped structure is found in the type I vestibular hair cells, that traverses the cell in an axial direction, extending towards the basement membrane. The present study...... estimates the total number of utricular hair cells and supporting cells in waltzing guinea pigs and age-matched control animals using the optical fractionator method. Animals were divided into four age groups (1, 7, 49 and 343 day-old). The number of type I hair cells decreased by 20% in the 343 day......-old waltzing guinea pigs compared to age-matched controls and younger animals. Two-photon confocal laser scanning microscopy using antibodies against fimbrin and betaIII-tubulin showed that the rods were exclusive to type I hair cells. There was no significant change in the length of the filament rods with age...

  3. del cambio

    Directory of Open Access Journals (Sweden)

    Alejandro Canales

    2006-01-01

    Full Text Available El artículo analiza las propuestas y los compromisos en materia de cobertura y de financiamiento en el periodo 2000-2006. En particular, revisa las modificaciones y los ajustes de los tres principales documentos en los que se expresaron las iniciativas: el de la Asociación Nacional de Universidades e Instituciones de Educación Superior, el del equipo de la transición y el programa sectorial. Los resultados muestran que las cifras de atención a la demanda educativa e inversión quedaron lejos de las metas autoimpuestas por el gobierno federal y que será necesario realizar mayores esfuerzos para atender tales desafíos.

  4. Wild genius - domestic fool? Spatial learning abilities of wild and domestic guinea pigs

    OpenAIRE

    Sachser Norbert; Pickel Thorsten; Lewejohann Lars; Kaiser Sylvia

    2010-01-01

    Abstract Background Domestic animals and their wild relatives differ in a wide variety of aspects. The process of domestication of the domestic guinea pig (Cavia aperea f. porcellus), starting at least 4500 years ago, led to changes in the anatomy, physiology, and behaviour compared with their wild relative, the wild cavy, Cavia aperea. Although domestic guinea pigs are widely used as a laboratory animal, learning and memory capabilities are often disregarded as being very scarce. Even less i...

  5. A Survey on the Gastrointestinal Parasites of Rabbit and Guinea Pig in a Laboratory Animal House

    OpenAIRE

    Motamedi, G.,; Moharami, M.,; Paykari, H.,; Eslampanah, M.,; Omraninava, A.

    2014-01-01

    There is documented evidence that infection in laboratory animals can often influence the outcome of experiments. All infections, apparent or inapparent, are likely to increase biological variability. As a research project concerning the diversity and distribution of parasites of rabbit and guinea pig in a conventional laboratory animal house, about 87 rabbits (from 700 ) and 105 guinea pigs (from 1500 ) were selected randomly from a Research, Production & Breeding of Laboratory Animals Depar...

  6. RESEARCH PRIORITY-SETTING IN PAPUA NEW GUINEA: POLICIES, METHODS AND PRACTICALITIES

    OpenAIRE

    Omuru, Eric; Kingwell, Ross S.

    2000-01-01

    Agricultural research priority-setting at best promotes the effective and efficient use of scarce research resources. This paper reviews firstly the priority-setting methods used in Papua New Guinea for agricultural R&D and examines the practicalities of implementing these and other methods. Secondly, this paper reports on key factors affecting the strategic directions for agricultural R&D in Papua New Guinea. These factors include:(i) the long term trends in international crop prices; (ii) l...

  7. Molecular Cloning, Expression, and In Silico Structural Analysis of Guinea Pig IL-17

    OpenAIRE

    Dirisala, Vijaya R.; Jeevan, Amminikutty; Ramasamy, Suresh K.; McMurray, David N.

    2013-01-01

    Interleukin-17A (IL-17A) is a potent proinflammatory cytokine and the signature cytokine of Th17 cells, a subset which is involved in cytokine and chemokine production, neutrophil recruitment, promotion of T cell priming, and antibody production. IL-17 may play an important role in tuberculosis and other infectious diseases. In preparation for investigating its role in the highly relevant guinea pig model of pulmonary tuberculosis, we cloned guinea pig IL-17A for the first time. The complete ...

  8. A Pilot Study of Uterine Artery Embolization with Tris-Acryl Gelatin Microspheres in Guinea Pigs

    International Nuclear Information System (INIS)

    Zhuang Wenquan; Tan Guosheng; Guo Wenbo; Yang Jianyong

    2012-01-01

    Objective: This study was designed to establish guinea pigs as an animal model for uterine artery embolization (UAE) with tris-acryl gelatin microspheres (TAGM). Methods: Twenty-five female adult guinea pigs were randomly divided into two groups, including a uterine artery casting mould group (n = 10) and a UAE group (n = 15). Pelvic angiography and vascular casting mould were performed in the first group. The anatomical characters of the pelvic cavity in guinea pigs were described. In the second group, the technical feasibility of performing UAE with TAGM in guinea pigs was investigated. The histopathological slides of the uterus of guinea pigs after UAE were examined to inspect the outcomes of UAE. Results: The uterine artery springs from the internal iliac artery, ascends tortuously along the cervix, and gives off vertically 8–10 branches to the cervix uteri and uterine horns. The diameters of the trunk of the uterine artery and its first branch were 0.32 ± 0.027 mm and 0.14 ± 0.01 mm, respectively. For UAE animals, the dosages of 40–120 and 100–300 μm TAGM were 0.033 ± 0.003 ml and 0.015 ± 0.002 ml, respectively. On histopathological slides, embosphere particles were found in the first branches of the uterine artery, the subserous arteries, and the intramural arteries. Inflammatory reactions in the uterus were common in guinea pigs after UAE. Local or dispersed areas of necrosis in uterus also were observed in a few guinea pigs. Conclusions: Guinea pigs are an appropriate and feasible model for UAE with TAGM.

  9. "Stuck in thorns". The effects of power on Liberian refugees in Guinea

    OpenAIRE

    Collins, Henry Ahorttor

    2013-01-01

    Abstract This thesis is about refugees who fled from Liberia to Guinea to join the miserable sea of humanity" (Malkki 1996) from January 1990 when the Charles Taylor led a rebel war that began in December 1989. Essentially, this thesis focuses on analyzing how first the bureaucratic power exercised by UNHCR and CNISR, as well as second, everyday power by Guinean majority affect Liberian refugees in Guinea, and how the refugees themself have found ways to address the challenges they meet. ...

  10. Chamber-specific effects of hypokalaemia on ventricular arrhythmogenicity in isolated, perfused guinea-pig heart

    DEFF Research Database (Denmark)

    Osadchii, Oleg E; Bentzen, Bo Hjorth; Olesen, Søren-Peter

    2009-01-01

    of hypokalaemic perfusion (2.5 mm K(+) for 30 min) were assessed in isolated guinea-pig heart preparations using simultaneous recordings of volume-conducted electrocardiogram and monophasic action potentials from six ventricular epicardial sites. Effective refractory periods, ventricular fibrillation thresholds...... for re-entrant tachyarrhythmias. Taken together, these findings suggest that proarrhythmic effects of hypokalaemia are mostly attributed to increased LV arrhythmogenicity in the guinea-pig heart....

  11. Maritime Security and Capacity Building in The Gulf of Guinea

    DEFF Research Database (Denmark)

    Jacobsen, Katja Lindskov

    2017-01-01

    It is widely acknowledged that maritime security in the Gulf of Guinea is a highly complex phenomenon, involving a variety issues (legal deficiencies, inadequate military equipment, and challenges like corruption, political unrest, youth unemployment etc.) as well as a multiplicity of external...... for a comprehensive approach, as well as the difficulties of translating the potential for comprehensiveness into practice (as will be shown, important aspects of the problem remain largely unaddressed). What is more, we also need to appreciate that, even if these gaps represent a ‘failure’ to deliver a comprehensive...... response, they are at the same time illustrative of how the maritime capacity building activities of various external actors also ‘succeed’ in having an impact on this regional security landscape – for instance by influencing how certain aspects of this multifaceted problem are prioritized, whilst others...

  12. Challenges facing HIV treatment in Guinea-Bissau

    DEFF Research Database (Denmark)

    Jespersen, Sanne; Hønge, Bo Langhoff; Oliveira, Inés

    2014-01-01

    PROBLEM: The introduction of antiretroviral therapy (ART) for HIV infection in sub-Saharan Africa has improved the quality of life of millions of people and reduced mortality. However, substantial problems with the infrastructure for ART delivery remain. APPROACH: Clinicians and researchers...... at an HIV clinic in Guinea-Bissau identified problems with the delivery of ART by establishing a clinical database and by collaborating with international researchers. LOCAL SETTING: The Bissau HIV cohort study group was established in 2007 as a collaboration between local HIV physicians and international...... HIV researchers. Patients were recruited from the HIV clinic at the country's main hospital in the capital Bissau. RELEVANT CHANGES: Between 2005 and 2013, 5514 HIV-positive patients were treated at the clinic. Working together, local health-care workers and international researchers identified...

  13. Resveratrol anti-ultraviolet-induced guinea pig skin injury

    International Nuclear Information System (INIS)

    Li Wenxing; Zhao Ying

    2014-01-01

    Objective: To Estimate on the protection effect of Stilbene on skin damage induced by ultraviolet radiation. Methods: After the normal skin in guinea pig under the intervene of Resveratrol was irradiated with over- dose of ultraviolet rays (UVB and UVA), the samples in every group were matched and compared. Results: The skin tissue in the Resveratrol intervene group irradiated by ultraviolet rays didn't change obviously as compared with that in the self-control group. But, the damage skin tissue in the control group irradiated by ultraviolet did change significantly as compared with that in the Stilbene intervene group. Conclusion: Resveratrol is a good material to protect the skin from damage effect by ultraviolet radiation. (authors)

  14. Mobile phone-based syndromic surveillance system, Papua New Guinea.

    Science.gov (United States)

    Rosewell, Alexander; Ropa, Berry; Randall, Heather; Dagina, Rosheila; Hurim, Samuel; Bieb, Sibauk; Datta, Siddhartha; Ramamurthy, Sundar; Mola, Glen; Zwi, Anthony B; Ray, Pradeep; MacIntyre, C Raina

    2013-11-01

    The health care system in Papua New Guinea is fragile, and surveillance systems infrequently meet international standards. To strengthen outbreak identification, health authorities piloted a mobile phone-based syndromic surveillance system and used established frameworks to evaluate whether the system was meeting objectives. Stakeholder experience was investigated by using standardized questionnaires and focus groups. Nine sites reported data that included 7 outbreaks and 92 cases of acute watery diarrhea. The new system was more timely (2.4 vs. 84 days), complete (70% vs. 40%), and sensitive (95% vs. 26%) than existing systems. The system was simple, stable, useful, and acceptable; however, feedback and subnational involvement were weak. A simple syndromic surveillance system implemented in a fragile state enabled more timely, complete, and sensitive data reporting for disease risk assessment. Feedback and provincial involvement require improvement. Use of mobile phone technology might improve the timeliness and efficiency of public health surveillance.

  15. Mobile Phone–based Syndromic Surveillance System, Papua New Guinea

    Science.gov (United States)

    Ropa, Berry; Randall, Heather; Dagina, Rosheila; Hurim, Samuel; Bieb, Sibauk; Datta, Siddhartha; Ramamurthy, Sundar; Mola, Glen; Zwi, Anthony B.; Ray, Pradeep; MacIntyre, C. Raina

    2013-01-01

    The health care system in Papua New Guinea is fragile, and surveillance systems infrequently meet international standards. To strengthen outbreak identification, health authorities piloted a mobile phone–based syndromic surveillance system and used established frameworks to evaluate whether the system was meeting objectives. Stakeholder experience was investigated by using standardized questionnaires and focus groups. Nine sites reported data that included 7 outbreaks and 92 cases of acute watery diarrhea. The new system was more timely (2.4 vs. 84 days), complete (70% vs. 40%), and sensitive (95% vs. 26%) than existing systems. The system was simple, stable, useful, and acceptable; however, feedback and subnational involvement were weak. A simple syndromic surveillance system implemented in a fragile state enabled more timely, complete, and sensitive data reporting for disease risk assessment. Feedback and provincial involvement require improvement. Use of mobile phone technology might improve the timeliness and efficiency of public health surveillance. PMID:24188144

  16. Plant regeneration from organ culture in white Guinea Yam

    International Nuclear Information System (INIS)

    Nwachukwu, E.C.; Mbanaso, E.N.A.; Sonnino, A.

    1997-01-01

    Explants from leaves, leaf segments, petioles and internodal stem of in vitro grown seedlings of white guinea yam, Dioscorea rotundata Poir, cv. 'Obiaoturugo' were cultured on defined media. NAA at concentrations of 0.5-1.0 mg/1 induced shoot regeneration from petiolar and inter-nodal stem pieces, and rooting occurred with little or no callusing from whole leaves or leaf segments. With concentration of 3.0-10.0 m/1 NAA, explants from petioles, inter-nodal stem, whole leaves and leaf segments formed callus which produced roots. These explants developed plantlets when subcultured on MS medium supplemented with 2.0 mg/1 BAP and 0.1 mg/1 NAA. (author). 11 refs, 1 tab

  17. [Ebola crisis in Guinea: psychosocial support for patients and caregivers].

    Science.gov (United States)

    Benifei, Sarah; Facon-Delahaye, Aline; Vautier, Virginie

    2016-02-01

    The experience of the French military health service in the fight against the Ebola epidemic in Guinea, highlights the importance of what favours the emergence of an institutional life in a context of care faced with numerous constraints and extraordinary challenges. The meticulous drawing up of procedures and the juxtaposition of expertise goes hand in hand with the construction of a triangular care system (caregivers-patients-families). This relational approach ensures each player in this system is able to find their place and a balance between constraints and satisfactions, losses and successes, isolation and support. This balance seems to favour individual and group resilience. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  18. Plant regeneration from organ culture in white Guinea Yam

    Energy Technology Data Exchange (ETDEWEB)

    Nwachukwu, E C; Mbanaso, E N.A. [National Root Crops Research Inst., Umudike, Umuahia, Abia State (Nigeria); Sonnino, A [Centro Recerche Energia, ENEA, Rome (Italy)

    1997-07-01

    Explants from leaves, leaf segments, petioles and internodal stem of in vitro grown seedlings of white guinea yam, Dioscorea rotundata Poir, cv. `Obiaoturugo` were cultured on defined media. NAA at concentrations of 0.5-1.0 mg/1 induced shoot regeneration from petiolar and inter-nodal stem pieces, and rooting occurred with little or no callusing from whole leaves or leaf segments. With concentration of 3.0-10.0 m/1 NAA, explants from petioles, inter-nodal stem, whole leaves and leaf segments formed callus which produced roots. These explants developed plantlets when subcultured on MS medium supplemented with 2.0 mg/1 BAP and 0.1 mg/1 NAA. (author). 11 refs, 1 tab.

  19. Energy and the rural sector in Papua New Guinea

    Energy Technology Data Exchange (ETDEWEB)

    Woodward, J L

    1977-03-15

    Energy in a dispersed form and at a low cost is needed if it is to have a significant effect in Papua, New Guinea. Appropriate application of energy at an intermediate-technology level would involve local residents in the construction and operation of solar and wind systems, inexpensive water systems, and a waste-fueled power system. The region's primary resource, manpower, must be utilized to overcome the disadvantages of poverty and a difficult terrain. Wood is the principal fuel and is burned directly as well as converted to producer gas. A review of the village's resources and potential for development concludes that medium-scale projects are feasible if the people are given guidance to help them develop technical and mechanical competence. 7 references. (DCK)

  20. Changing pattern of malaria in Bissau, Guinea Bissau

    DEFF Research Database (Denmark)

    Rodrigues, Amabelia; Schellenberg, Joanna Armstrong; Kofoed, Poul-Erik

    2008-01-01

    OBJECTIVE: To describe the epidemiology of malaria in Guinea-Bissau, in view of the fact that more funds are available now for malaria control in the country. METHODS: From May 2003 to May 2004, surveillance for malaria was conducted among children less than 5 years of age at three health centres...... covering the study area of the Bandim Health Project (BHP) and at the outpatient clinic of the national hospital in Bissau. Cross-sectional surveys were conducted in the community in different malaria seasons. RESULTS: Malaria was overdiagnosed in both health centres and hospital. Sixty-four per cent...... of the children who presented at a health centre were clinically diagnosed with malaria, but only 13% of outpatient children who tested for malaria had malaria parasitaemia. Only 44% (963/2193) of children admitted to hospital with a diagnosis of malaria had parasitaemia. The proportion of positive cases...

  1. Prenatal effects of trichlorfon on the guinea pig brain

    International Nuclear Information System (INIS)

    Berge, G.N.; Nafstad, I.; Fonnum, F.

    1986-01-01

    The organophosphorus compound trichlorfon, dimethyl (2,2,2-trichloro-1-hydroxyethyl) phosphonate, was administered by stomach tube (100 mg/kg) to pregnant guinea pigs at two different stages of gestation (days 36, 37, 38 and 51, 52, 53). The pups developed locomotory disturbances, and post mortem examination revealed significantly decreased weights of the total brain and the cerebellum, as compared to controls. There was also a significant weight reduction particularly of the medulla oblongata, but also of the hippocampus, the thalamus, and the colliculi. Histological examination of the cerebellum revealed reduction of the external granular layer and the molecular layer, and regional absence of Purkinje cells. The activities of the neutrotransmitter enzymes choline acetyltransferase (ChAT), and glutamate decarboxylase (GAD) in cerebellum were reduced as compared to the control values. (orig.)

  2. linear accelerator simulation framework with placet and guinea-pig

    CERN Document Server

    Snuverink, Jochem; CERN. Geneva. ATS Department

    2016-01-01

    Many good tracking tools are available for simulations for linear accelerators. However, several simple tasks need to be performed repeatedly, like lattice definitions, beam setup, output storage, etc. In addition, complex simulations can become unmanageable quite easily. A high level layer would therefore be beneficial. We propose LinSim, a linear accelerator framework with the codes PLACET and GUINEA-PIG. It provides a documented well-debugged high level layer of functionality. Users only need to provide the input settings and essential code and / or use some of the many implemented imperfections and algorithms. It can be especially useful for first-time users. Currently the following accelerators are implemented: ATF2, ILC, CLIC and FACET. This note is the comprehensive manual, discusses the framework design and shows its strength in some condensed examples.

  3. Transmission of Influenza B Viruses in the Guinea Pig

    Science.gov (United States)

    Pica, Natalie; Chou, Yi-Ying; Bouvier, Nicole M.

    2012-01-01

    Epidemic influenza is typically caused by infection with viruses of the A and B types and can result in substantial morbidity and mortality during a given season. Here we demonstrate that influenza B viruses can replicate in the upper respiratory tract of the guinea pig and that viruses of the two main lineages can be transmitted with 100% efficiency between inoculated and naïve animals in both contact and noncontact models. Our results also indicate that, like in the case for influenza A virus, transmission of influenza B viruses is enhanced at colder temperatures, providing an explanation for the seasonality of influenza epidemics in temperate climates. We therefore present, for the first time, a small animal model with which to study the underlying mechanisms of influenza B virus transmission. PMID:22301149

  4. MHealth to Improve Measles Immunization in Guinea-Bissau

    DEFF Research Database (Denmark)

    Olsen, Emil Rossing; Ravn, Henrik; Batista, Celso Soares Pereira

    2016-01-01

    BACKGROUND: Recent studies have revealed a low measles vaccination (MV) rate in the Republic of Guinea-Bissau (West Africa) that has not increased in accordance with the increasing coverage of other vaccinations. Measles is the deadliest of all childhood rash/fever illnesses and spreads easily...... receiving the MV, will be enrolled when they arrive with their children at the health center to receive the Bacillus Calmette-Guérin vaccination, usually within one month of the child's birth. Enrolment will continue until a study population of 990 children has been reached. The participants...... will be randomly assigned to a control arm or one of two intervention arms. Each of the three groups will have 330 participants, distributed equally between health centers. Participants in the first intervention arm will receive a scheduled short message service (SMS) text message reminding them of the MV...

  5. Pharmacology of Bradykinin-Evoked Coughing in Guinea Pigs.

    Science.gov (United States)

    Hewitt, Matthew M; Adams, Gregory; Mazzone, Stuart B; Mori, Nanako; Yu, Li; Canning, Brendan J

    2016-06-01

    Bradykinin has been implicated as a mediator of the acute pathophysiological and inflammatory consequences of respiratory tract infections and in exacerbations of chronic diseases such as asthma. Bradykinin may also be a trigger for the coughing associated with these and other conditions. We have thus set out to evaluate the pharmacology of bradykinin-evoked coughing in guinea pigs. When inhaled, bradykinin induced paroxysmal coughing that was abolished by the bradykinin B2 receptor antagonist HOE 140. These cough responses rapidly desensitized, consistent with reports of B2 receptor desensitization. Bradykinin-evoked cough was potentiated by inhibition of both neutral endopeptidase and angiotensin-converting enzyme (with thiorphan and captopril, respectively), but was largely unaffected by muscarinic or thromboxane receptor blockade (atropine and ICI 192605), cyclooxygenase, or nitric oxide synthase inhibition (meclofenamic acid and N(G)-nitro-L-arginine). Calcium influx studies in bronchopulmonary vagal afferent neurons dissociated from vagal sensory ganglia indicated that the tachykinin-containing C-fibers arising from the jugular ganglia mediate bradykinin-evoked coughing. Also implicating the jugular C-fibers was the observation that simultaneous blockade of neurokinin2 (NK2; SR48968) and NK3 (SR142801 or SB223412) receptors nearly abolished the bradykinin-evoked cough responses. The data suggest that bradykinin induces coughing in guinea pigs by activating B2 receptors on bronchopulmonary C-fibers. We speculate that therapeutics targeting the actions of bradykinin may prove useful in the treatment of cough. Copyright © 2016 by The American Society for Pharmacology and Experimental Therapeutics.

  6. Seasonal variation in child mortality in rural Guinea-Bissau.

    Science.gov (United States)

    Nielsen, Bibi Uhre; Byberg, Stine; Aaby, Peter; Rodrigues, Amabelia; Benn, Christine Stabell; Fisker, Ane Baerent

    2017-07-01

    In many African countries, child mortality is higher in the rainy season than in the dry season. We investigated the effect of season on child mortality by time periods, sex and age in rural Guinea-Bissau. Bandim health project follows children under-five in a health and demographic surveillance system in rural Guinea-Bissau. We compared the mortality in the rainy season (June to November) between 1990 and 2013 with the mortality in the dry season (December to May) in Cox proportional hazards models providing rainy vs. dry season mortality rate ratios (r/d-mrr). Seasonal effects were estimated in strata defined by time periods with different frequency of vaccination campaigns, sex and age (<1 month, 1-11 months, 12-59 months). Verbal autopsies were interpreted using InterVa-4 software. From 1990 to 2013, overall mortality was declined by almost two-thirds among 81 292 children (10 588 deaths). Mortality was 51% (95% ci: 45-58%) higher in the rainy season than in the dry season throughout the study period. The seasonal difference increased significantly with age, the r/d-mrr being 0.94 (0.86-1.03) among neonates, 1.57 (1.46-1.69) in post-neonatal infants and 1.83 (1.72-1.95) in under-five children (P for same effect <0.001). According to the InterVa, malaria deaths were the main reason for the seasonal mortality difference, causing 50% of all deaths in the rainy season, but only if the InterVa included season of death, making the argument self-confirmatory. The mortality declined throughout the study, yet rainy season continued to be associated with 51% higher overall mortality. © 2017 The Authors. Tropical Medicine & International Health Published by John Wiley & Sons Ltd.

  7. Microbial stasis of Leishmania enriettii in activated guinea pig macrophages

    International Nuclear Information System (INIS)

    Groocock, C.M.; Soulsby, E.J.L.

    1980-01-01

    Peritoneal exudate cells (PEC) from Leishmania-sensitized guinea pigs were cultured in vitro in the presence (activated) or absence (non-activated) of leishmanial antigen for 24 or 48 hours. These were then labelled with 51 Cr and challenged with 125 I-labelled promastigotes. The changing relationship between the macrophage and the parasite was monitored by observing changes in the ratio of the cell-associated isotopes. Highly significant differences in the ratio change developed during culture. These differences were a result of the activated cultures showing a higher release of 51 Cr and a lower release of 125 I when compared with the non-activated cells, at 12 hours the percentage release of 125 I from the parasite within the activated macrophage was fourfold less than that released by parasites within non-activated cells (9.2% versus 38.3%) and tenfold less than that released from glutaraldehyde-killed organisms phagocytosed by activated macrophages (91.6%). These studies indicate that stasis rather than killing of leishmaniae occurs in the activated macrophage in vitro. Parallel experiments evaluated by the visual counting of leishmaniae within the macrophages support these data. PEC from tuberculin-sensitized guinea pigs activated in vitro by purified protein derivative showed little or no activity against leishmaniae, indicating a specific requirement for this microbial stasis by activated macrophages. As a corollary of this, peritoneal exudate lymphocytes separated from the same preparations of PEC were shown to be specifically reactive to leishmanial antigen by transformation and incorporation of 3 H-thymidine. (author)

  8. Comparative phylogeography in rainforest trees from Lower Guinea, Africa.

    Directory of Open Access Journals (Sweden)

    Myriam Heuertz

    Full Text Available Comparative phylogeography is an effective approach to assess the evolutionary history of biological communities. We used comparative phylogeography in fourteen tree taxa from Lower Guinea (Atlantic Equatorial Africa to test for congruence with two simple evolutionary scenarios based on physio-climatic features 1 the W-E environmental gradient and 2 the N-S seasonal inversion, which determine climatic and seasonality differences in the region. We sequenced the trnC-ycf6 plastid DNA region using a dual sampling strategy: fourteen taxa with small sample sizes (dataset 1, mean n = 16/taxon, to assess whether a strong general pattern of allele endemism and genetic differentiation emerged; and four taxonomically well-studied species with larger sample sizes (dataset 2, mean n = 109/species to detect the presence of particular shared phylogeographic patterns. When grouping the samples into two alternative sets of two populations, W and E, vs. N and S, neither dataset exhibited a strong pattern of allelic endemism, suggesting that none of the considered regions consistently harboured older populations. Differentiation in dataset 1 was similarly strong between W and E as between N and S, with 3-5 significant F ST tests out of 14 tests in each scenario. Coalescent simulations indicated that, given the power of the data, this result probably reflects idiosyncratic histories of the taxa, or a weak common differentiation pattern (possibly with population substructure undetectable across taxa in dataset 1. Dataset 2 identified a common genetic break separating the northern and southern populations of Greenwayodendron suaveolens subsp. suaveolens var. suaveolens, Milicia excelsa, Symphonia globulifera and Trichoscypha acuminata in Lower Guinea, in agreement with differentiation across the N-S seasonal inversion. Our work suggests that currently recognized tree taxa or suspected species complexes can contain strongly differentiated genetic lineages

  9. Immunomodulation of afferent neurons in guinea-pig isolated airway.

    Science.gov (United States)

    Riccio, M M; Myers, A C; Undem, B J

    1996-03-01

    1. The trachea, larynx and main bronchi with the right vagus nerve and nodose ganglion were isolated from guinea-pigs passively immunized 24 h previously with serum containing anti-ovalbumin antibody. 2. The airways were placed in one compartment of a Perspex chamber for recording of isometric tension while the nodose ganglion and attached vagus nerve were pulled into another compartment. Action potentials arriving from single airway afferent nerve endings were monitored extracellularly using a glass microelectrode positioned near neuronal cell bodies in the ganglion. Mechanosensitivity of the nerve endings was quantified using calibrated von Frey filaments immediately before and after exposure to antigen (10 micrograms ml-1 ovalbumin). 3. Ten endings responded to the force exerted by the lowest filament (0.078 mN) and were not further investigated. In airways from thirteen immunized guinea-pigs, the mechanical sensitivity of A delta afferent fibres (conduction velocity = 4.3 +/- 0.6 m s-1) was enhanced 4.1 +/- 0.9-fold following airway exposure to antigen (P action potential generation except in one instance when the receptive field was located over the smooth muscle. This ending also responded to methacholine suggesting that spatial changes in the receptive field, induced by muscle contraction, were responsible for the activation. 5. The mediators responsible for these effects are unknown, although histamine, prostaglandins, leukotrienes and tachykinins do not appear to be essential. The increase in mechanical responsiveness was not associated with the smooth muscle contraction since leukotriene C4, histamine and tachykinins, which all caused a similar contraction to antigen, did not affect mechanical thresholds. Moreover, the antigen-induced increases in excitability persisted beyond the duration of the smooth muscle contraction. 6. These results demonstrate that antigen-antibody-mediated inflammatory processes may enhance the excitability of vagal afferent

  10. Growth and breast-milk intake in Papua New Guinea

    International Nuclear Information System (INIS)

    Coward, W.A.

    2000-01-01

    The purpose of this work is to show what is possible if lactation performance and growth are measured in the context of the WHO Growth Reference Study. The fact is that a piece of work very similar to that which could be reasonably proposed for the present study was done in the 1980's in Papua New Guinea (PNG) and reading Alison Orr-Ewing's thesis it is interesting to find the statement 'The apparent adequacy of mean nutrient intakes up to 6 months and the small and late effect of morbidity on weight gain observed in this study, both substantiate the suggestion that the growth faltering relative to international standards which occurs from 3-5 months is probably largely due to the inappropriate nature of the standards'. This sort of finding was emerging from many research centres at that time (see for example Whitehead and Paul, in my own department) and provided the impetus to develop new standards for growth such as in the WHO Growth Reference Programme. At the same sort of time too, both myself and Dr Peter Heywood, then at the Papua New Guinea Institute of Medical Research were attending one of the first meetings for nutritionists held at IAEA in Vienna. There is thus a certain satisfaction that can be derived from the appropriateness of describing the work at this meeting but it must be tinged with regret that it has taken nearly 20 years to achieve it. The published work from PNG deals only with lactation but the present report will contain some unpublished material on growth and morbidity

  11. Blast cells transfer experimental hypersensitivity pneumonitis in guinea pigs

    International Nuclear Information System (INIS)

    Schuyler, M.; Cook, C.; Listrom, M.; Fengolio-Preiser, C.

    1988-01-01

    We previously demonstrated that experimental hypersensitivity pneumonitis (HP) can be transferred by lymph node cells (LNC) cultured in vitro with antigen. The purpose of this study was to identify the cells responsible for transfer and to determine if pulmonary cells can transfer HP. We cultured LNC from sensitized Strain 2 guinea pigs with a soluble extract of Micropolyspora faeni for 72 h, separated lymphoblasts from small lymphocytes, and transferred both subpopulations intravenously to syngeneic recipients. We also transferred irradiated lymphoblasts (1,500 rads), macrophage-depleted, lymphoblast-enriched populations, and pulmonary cells either without culture or after culture with M. faeni. Control animals received an equal volume of medium. All recipient animals were challenged intratracheally (i.t.) with M. faeni 48 h after the cell transfer, and they were killed 4 days after i.t. challenge. Randomly selected microscopic fields of the lung (250/animal) were judged to be normal or abnormal without knowledge of treatment. This measurement was reproducible (r = 0.95 for duplicate measurements, n = 55). All guinea pigs were maintained in HEPA-filtered air. There was a low level of pulmonary response to an i.t. challenge of M. faeni in animals that received medium. Animals that received pulmonary cells, either cultured or noncultured, did not differ from those in the control group. There was a substantial increase (p less than 0.01) in the extent of pulmonary abnormalities in the recipients of the lymphoblast population, with significant correlation (r = 0.87, p less than 0.01) between the number of lymphoblasts transferred and the extent of pulmonary abnormalities

  12. Pharmacokinetics of activated protein C in guinea pigs

    International Nuclear Information System (INIS)

    Berger, H. Jr.; Kirstein, C.G.; Orthner, C.L.

    1991-01-01

    Protein C is a vitamin K-dependent zymogen of the serine protease, activated protein C (APC), an important regulatory enzyme in hemostasis. In view of the potential of human APC as an anticoagulant and profibrinolytic agent, the pharmacokinetics and tissue distribution of APC were studied in guinea pigs. The plasma elimination of a trace dose of 125 I-APC was biphasic following an initial rapid elimination of approximately 15% of the injected dose within 1 to 2 minutes. This rapid removal of 125 I-APC from the circulation was found to be a result of an association with the liver regardless of the route of injection. Essentially identical results were obtained with active site-blocked forms of APC generated with either diisopropylfluorophosphate or D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone, which indicates that the active site was not essential for the liver association. Accumulation of all three forms of APC in the liver peaked at 30 minutes and then declined as increasing amounts of degraded radiolabeled material appeared in the gastrointestinal tract and urine. Removal of the gamma-carboxyglutamic acid (gla) domain of diisopropylphosphoryl-APC resulted in a 50% reduction in the association with liver and an accumulation in the kidneys. Protein C and protein S were cleared from the circulation at rates approximately one-half and one-fourth, respectively, that of APC. Both in vitro and in vivo, APC was found to form complexes with protease inhibitors present in guinea pig plasma. Complex formation resulted in a more rapid disappearance of the enzymatic activity of APC than elimination of the protein moiety. These findings indicate two distinct mechanisms for the elimination of APC. One mechanism involves reaction with plasma protease inhibitors and subsequent elimination by specific hepatic receptors. (Abstract Truncated)

  13. Anti-anaphylactic action of nordihydroguaiaretic acid in antigen sensitized guinea pigs.

    Science.gov (United States)

    Bergren, Dale R; Valentine, Jimmie L

    2016-12-01

    Therapeutic natural products and medicinal herbs has gained popularity. The anti-antigenic action of the plant alkaloid nordihydroguaiaretic acid (NDGA) was studied in ovalbumin (OA)-sensitized guinea pigs. In one series of experiments conscious, non-sedated guinea pigs were challenged with OA aerosol. Specific airway resistance (SR AW ) was monitored using a two-chambered whole-body plethysmograph. OA aerosol increased SR AW above that produced by vehicle administration. Prior NDGA administration by a 1min 0.9% aerosol (w/vol) attenuated the increase in SR AW resulting from OA challenge. In the anesthetized guinea pig pretreated with indomethacin, pyrilamine and propranolol, intravenous OA injection increased intra-tracheal pressure above vehicle injection. Intravenous NDGA administration (5mg/kg) reduced the intra-tracheal pressure increases. In a third series of experiments plasma leukotriene C 4 was measured by radio-immunoassay in 3 groups challenged with OA aerosol: vehicle-treated OA-sensitized, OA-sensitized receiving NDGA and vehicle treated guinea pigs. NDGA pretreatment reduced plasma LTC 4 in response to OA challenge in OA sensitized guinea pigs. This study demonstrates that NDGA is an effective antigenic agent when given by aerosol or intravenous injection in either conscious or anesthetized guinea pigs, respectively. The mechanism of action of NDGA is presumed primarily be due to the blockage of 5-lipoxygenase and therefore the synthesis of leukotrienes. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Increased Severity of Tuberculosis in Guinea Pigs with Type 2 Diabetes

    Science.gov (United States)

    Podell, Brendan K.; Ackart, David F.; Obregon-Henao, Andres; Eck, Sarah P.; Henao-Tamayo, Marcela; Richardson, Michael; Orme, Ian M.; Ordway, Diane J.; Basaraba, Randall J.

    2015-01-01

    Impaired glucose tolerance and type 2 diabetes were induced in guinea pigs to model the emerging comorbidity of Mycobacterium tuberculosis infection in diabetic patients. Type 2 diabetes mellitus was induced by low-dose streptozotocin in guinea pigs rendered glucose intolerant by first feeding a high-fat, high-carbohydrate diet before M. tuberculosis exposure. M. tuberculosis infection of diabetic guinea pigs resulted in severe and rapidly progressive tuberculosis (TB) with a shortened survival interval, more severe pulmonary and extrapulmonary pathology, and a higher bacterial burden compared with glucose-intolerant and nondiabetic controls. Compared with nondiabetics, diabetic guinea pigs with TB had an exacerbated proinflammatory response with more severe granulocytic inflammation and higher gene expression for the cytokines/chemokines interferon-γ, IL-17A, IL-8, and IL-10 in the lung and for interferon-γ, tumor necrosis factor-α, IL-8, and monocyte chemoattractant protein-1 in the spleen. TB disease progression in guinea pigs with impaired glucose tolerance was similar to that of nondiabetic controls in the early stages of infection but was more severe by day 90. The guinea pig model of type 2 diabetes–TB comorbidity mimics important features of the naturally occurring disease in humans. This model will be beneficial in understanding the complex pathogenesis of TB in diabetic patients and to test new strategies to improve TB and diabetes control when the two diseases occur together. PMID:24492198

  15. Serial histopathological changes in irradiated guinea pig lung receiving conventional fractionated and hyperfractionated irradiation

    International Nuclear Information System (INIS)

    Itoh, Satoshi; Inomata, Taisuke; Ogawa, Yasuhiro; Yoshida, Shoji; Sonobe, Hiroshi; Ohtsuki, Yuji

    1999-01-01

    The purpose of this study is to determine serial histopathological differences in guinea pig lungs receiving the same total dose as clinically used between conventional fractionated and hyperfractionated irradiation. The guinea pigs received 80 Gy in 40 daily fractions of 2 Gy each (conventional fractionation), 80 Gy in 80 fractions of 1 Gy each twice a day (hyperfractionation), 81 Gy in 27 daily fractions of 3 Gy each (conventional fractionation), or 81 Gy in 54 fractions of 1.5 Gy each twice a day (hyperfractionation). We evaluated the histopathological changes of irradiated guinea pig lungs at 1, 2, 3, 6, 9, and 12 months after irradiation. The guinea pig lungs that received 81 Gy in 27 daily fractions showed histopathological changes of inflammation including formation of lymph follicles after 6 months. The lungs which received 81 Gy in 54 fractions showed similar but slightly less pronounced changes than those that received 81 Gy in 27 daily fractions. The guinea pig lungs of other groups showed no histopathological changes during the observation period. In hyperfractionated irradiation the damage to the guinea pig lung is quantitatively less than that occurring as a result of conventional fractionated irradiation of the same total dose. (author)

  16. Immunogenicity of guinea pig cells transformed in culture by chemical carcinogens.

    Science.gov (United States)

    Ohanian, S H; McCabe, R P; Evans, C H

    1981-12-01

    The immunogenicity of inbred strain 2/N guinea pig fibroblasts transformed to the malignant state in vitro by chemical carcinogens was evaluated with the use of a variety of in vivo and in vitro methods including delayed-type hypersensitivity skin and tumor transplantation tests and analysis of antibody production by immunofluorescence, complement fixation, and staphylococcal protein A binding tests. Neoplastic transformation was induced by direct treatment of cells in culture with benzo[a]pyrene, 3-methylcholanthrene, or N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or by the host-mediated method by which fetuses were exposed to diethylnitrosamine or MNNG in vivo prior to cell culture. Rabbits and syngeneic guinea pigs were inoculated with unirradiated and X-irradiated clonally derived cells. Delayed hypersensitivity skin reactions to immunizing or other cells were equivalent in immunized or control guinea pigs, and no protection to tumor outgrowth from a challenge inoculum of immunizing cells was observed. Antibody activity induced in the sera of immunized guinea pigs was cross-reactive and removed by absorption with nontumorigenic cells. Rabbit antisera after absorption with fetal guinea pig cells were nonreactive with the specific immunizing or other culture cells. Chemical carcinogen-induced neoplastic transformation of guinea pig cells can, therefore, occur without formation of detectable, individually distinct cell surface tumor-specific neoantigens.

  17. Serial histopathological changes in irradiated guinea pig lung receiving conventional fractionated and hyperfractionated irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Itoh, Satoshi; Inomata, Taisuke; Ogawa, Yasuhiro; Yoshida, Shoji; Sonobe, Hiroshi; Ohtsuki, Yuji [Kochi Medical School, Nankoku (Japan)

    1999-05-01

    The purpose of this study is to determine serial histopathological differences in guinea pig lungs receiving the same total dose as clinically used between conventional fractionated and hyperfractionated irradiation. The guinea pigs received 80 Gy in 40 daily fractions of 2 Gy each (conventional fractionation), 80 Gy in 80 fractions of 1 Gy each twice a day (hyperfractionation), 81 Gy in 27 daily fractions of 3 Gy each (conventional fractionation), or 81 Gy in 54 fractions of 1.5 Gy each twice a day (hyperfractionation). We evaluated the histopathological changes of irradiated guinea pig lungs at 1, 2, 3, 6, 9, and 12 months after irradiation. The guinea pig lungs that received 81 Gy in 27 daily fractions showed histopathological changes of inflammation including formation of lymph follicles after 6 months. The lungs which received 81 Gy in 54 fractions showed similar but slightly less pronounced changes than those that received 81 Gy in 27 daily fractions. The guinea pig lungs of other groups showed no histopathological changes during the observation period. In hyperfractionated irradiation the damage to the guinea pig lung is quantitatively less than that occurring as a result of conventional fractionated irradiation of the same total dose. (author)

  18. Immunogenicity of guinea pig cells transformed in culture by chemical carcinogens

    International Nuclear Information System (INIS)

    Ohanian, S.H.; McCabe, R.P.; Evans, C.H.

    1981-01-01

    The immunogenicity of inbred strain 2/N guinea pig fibroblasts transformed to the malignant state in vitro by chemical carcinogens was evaluated with the use of a variety of in vivo and in vitro methods including delayed-type hypersensitivity skin and tumor transplantation tests and analysis of antibody production by immunofluorescence, complement fixation, and staphylococcal protein A binding tests. Neoplastic transformation was induced by direct treatment of cells in culture with benzo[a]pyrene, 3-methylcholanthrene, or N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or by the host-mediated method by which fetuses were exposed to diethylnitrosamine or MNNG in vivo prior to cell culture. Rabbits and syngeneic guinea pigs were inoculated with unirradiated and X-irradiated clonally derived cells. Delayed hypersensitivity skin reactions to immunizing or other cells were equivalent in immunized or control guinea pigs, and no protection to tumor outgrowth from a challenge inoculum of immunizing cells was observed. Antibody activity induced in the sera of immunized guinea pigs was cross-reactive and removed by absorption with nontumorigenic cells. Rabbit anitsera after absorption with fetal guinea pig cells were nonreactive with the specific immunizing or other cultured cells. Chemical carcinogen-induced neoplastic transformation of guinea pig cells can, therefore, occur without formation of detectable, individually distinct cell surface tumor-specific neoantigens

  19. Characterization of fetal growth by repeated ultrasound measurements in the wild guinea pig (Cavia aperea).

    Science.gov (United States)

    Schumann, K; Guenther, A; Göritz, F; Jewgenow, K

    2014-08-01

    Fetal growth during pregnancy has previously been studied in the domesticated guinea pig (Cavia aperea f. porcellus) after dissecting pregnant females, but there are no studies describing the fetal growth in their wild progenitor, the wild guinea pig (C aperea). In this study, 50 pregnancies of wild guinea pig sows were investigated using modern ultrasound technique. The two most common fetal growth parameters (biparietal diameter [BPD] and crown-rump-length [CRL]) and uterine position were measured. Data revealed similar fetal growth patterns in the wild guinea pig and domesticated guinea pig in the investigated gestation period, although they differ in reproductive milestones such as gestation length (average duration of pregnancy 68 days), average birth weight, and litter mass. In this study, pregnancy lasted on average 60.2 days with a variance of less than a day (0.96 days). The measured fetal growth parameters are strongly correlated with each (R = 0.91; P guinea pig. Copyright © 2014 Elsevier Inc. All rights reserved.

  20. Protein A Suppresses Immune Responses during Staphylococcus aureus Bloodstream Infection in Guinea Pigs

    Science.gov (United States)

    Kim, Hwan Keun; Falugi, Fabiana; Thomer, Lena; Missiakas, Dominique M.

    2015-01-01

    ABSTRACT   Staphylococcus aureus infection is not associated with the development of protective immunity, and disease relapses occur frequently. We hypothesize that protein A, a factor that binds immunoglobulin Fcγ and cross-links VH3 clan B cell receptors (IgM), is the staphylococcal determinant for host immune suppression. To test this, vertebrate IgM was examined for protein A cross-linking. High VH3 binding activity occurred with human and guinea immunoglobulin, whereas mouse and rabbit immunoglobulins displayed little and no binding, respectively. Establishing a guinea pig model of S. aureus bloodstream infection, we show that protein A functions as a virulence determinant and suppresses host B cell responses. Immunization with SpAKKAA, which cannot bind immunoglobulin, elicits neutralizing antibodies that enable guinea pigs to develop protective immunity. Importance  Staphylococcus aureus is the leading cause of soft tissue and bloodstream infections; however, a vaccine with clinical efficacy is not available. Using mice to model staphylococcal infection, earlier work identified protective antigens; however, corresponding human clinical trials did not reach their endpoints. We show that B cell receptor (IgM) cross-linking by protein A is an important immune evasion strategy of S. aureus that can be monitored in a guinea pig model of bloodstream infection. Further, immunization with nontoxigenic protein A enables infected guinea pigs to elicit antibody responses that are protective against S. aureus. Thus, the guinea pig model may support preclinical development of staphylococcal vaccines. PMID:25564466

  1. Chlamydia caviae infection alters abundance but not composition of the guinea pig vaginal microbiota.

    Science.gov (United States)

    Neuendorf, Elizabeth; Gajer, Pawel; Bowlin, Anne K; Marques, Patricia X; Ma, Bing; Yang, Hongqiu; Fu, Li; Humphrys, Michael S; Forney, Larry J; Myers, Garry S A; Bavoil, Patrik M; Rank, Roger G; Ravel, Jacques

    2015-06-01

    In humans, the vaginal microbiota is thought to be the first line of defense again pathogens including Chlamydia trachomatis. The guinea pig has been extensively used as a model to study chlamydial infection because it shares anatomical and physiological similarities with humans, such as a squamous vaginal epithelium as well as some of the long-term outcomes caused by chlamydial infection. In this study, we aimed to evaluate the guinea pig-C. caviae model of genital infection as a surrogate for studying the role of the vaginal microbiota in the early steps of C. trachomatis infection in humans. We used culture-independent molecular methods to characterize the relative and absolute abundance of bacterial phylotypes in the guinea pig vaginal microbiota in animals non-infected, mock-infected or infected by C. caviae. We showed that the guinea pig and human vaginal microbiotas are of different bacterial composition and abundance. Chlamydia caviae infection had a profound effect on the absolute abundance of bacterial phylotypes but not on the composition of the guinea pig vaginal microbiota. Our findings compromise the validity of the guinea pig-C. caviae model to study the role of the vaginal microbiota during the early steps of sexually transmitted infection. © FEMS 2015.

  2. Protective effects of isorhynchophylline on cardiac arrhythmias in rats and guinea pigs.

    Science.gov (United States)

    Gan, Runtao; Dong, Guo; Yu, Jiangbo; Wang, Xu; Fu, Songbin; Yang, Shusen

    2011-09-01

    As one important constituent extracted from a traditional Chinese medicine, Uncaria Rhynchophylla Miq Jacks, isorhynchophylline has been used to treat hypertension, epilepsy, headache, and other illnesses. Whether isorhynchophylline protects hearts against cardiac arrhythmias is still incompletely investigated. This study was therefore aimed to examine the preventive effects of isorhynchophylline on heart arrhythmias in guinea pigs and rats and then explore their electrophysiological mechanisms. In vivo, ouabain and calcium chloride were used to establish experimental arrhythmic models in guinea pigs and rats. In vitro, the whole-cell patch-lamp technique was used to study the effect of isorhynchophylline on action potential duration and calcium channels in acutely isolated guinea pig and rat cardiomyocytes. The dose of ouabain required to induce cardiac arrhythmias was much larger in guinea pigs administered with isorhynchophylline. Additionally, the onset time of cardiac arrhythmias induced by calcium chloride was prolonged, and the duration was shortened in rats pretreated with isorhynchophylline. The further study showed that isorhynchophylline could significantly decrease action potential duration and inhibit calcium currents in isolated guinea pig and rat cardiomyocytes in a dose-dependent manner. In summary, isorhynchophylline played a remarkably preventive role in cardiac arrhythmias through the inhibition of calcium currents in rats and guinea pigs. © Georg Thieme Verlag KG Stuttgart · New York.

  3. Confocal Raman Microspectroscopy: The Measurement of VX Depth Profiles in Hairless Guinea Pig Skin and the Evaluation of RSDL

    Science.gov (United States)

    2015-02-01

    USAMRICD-TR-15-01 Confocal Raman Microspectroscopy: The Measurement of VX Depth Profiles in Hairless Guinea Pig Skin and the Evaluation...5a. CONTRACT NUMBER guinea pig skin and the evaluation of RSDL 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Braue, EH...upper skin layers of hairless guinea pigs and to determine the ability of Reactive Skin Decontamination Lotion (RSDL) to remove or degrade VX from

  4. Effects of Potassium Channel Blockers on the Negative Inotropic Responses Induced by Cromakalim and Pinacidil in Guinea Pig Atrium

    Science.gov (United States)

    1992-01-01

    RD-A2•4 875 EFFECTS OF POTASSIUM CHANNEL BLOCKERS ON THE NEGATIVE 1/1 INOTROPIC RESPONSES INDUCED BY CRONAKALIM RND PINACIDIL IN GUINEA PIG ATRIUM(U...INOTROPICTRSPONSES INDUCED BY CROMAKAUM AND PINACIDILIN GUINEA PIG ATRIUM a AUTHOR WAI-MAN LAU 7 FORMING ORG NAMES/ADDRESSES DEFENCE SCIENCE AND a...and Technology Organisaio Aot Val. Negative Inotropic Responses Victoria. Australia Induced by Cromakalim and Pinacidil in Guinea Pig Atrium Key

  5. Comparison of Four Skin Decontamination Procedures Using Reactive Skin Decontamination Lotion (RSDL) Following Cutaneous VX Exposure in Guinea Pigs

    Science.gov (United States)

    2016-01-01

    DC) product following cutaneous exposure to VX was affected by the DC procedure. Fur-clipped, male, unanesthetized guinea pigs were used as subjects...RSDL) Following Cutaneous VX Exposure in Guinea Pigs Irwin Koplovitz Susan Schulz Julia Morgan Robert Reed Edward Clarkson C. Gary Hurst...Decontamination Procedures Using Reactive Skin 5a. CONTRACT NUMBER Decontamination Lotion (RSDL) Following Cutaneous VX Exposure in Guinea Pigs 5b

  6. Median Lethal Doses Associated with Intravenous Exposure to the Optically Pure Enantiomers of VX in Guinea Pigs

    Science.gov (United States)

    2017-03-01

    in Guinea Pigs 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Wright, Linnzi K. M.; Forster, Jeffry S...intravenous exposure of adult, male guinea pigs to the individual VX enantiomers, and we compared those potencies to that for a racemic mixture. The P...highly toxic based on the U.S. Environmental Protection Agency’s Acute Toxicity Categories for Pesticide Products. 15. SUBJECT TERMS Guinea pig

  7. Resultado econômico da produção de ovinos para carne em pasto de azevém e confinamento - DOI: 10.4025/actascianimsci.v31i1.3995 Economic results of sheep production on ryegrass pasture or feedlot - DOI: 10.4025/actascianimsci.v31i1.3995

    Directory of Open Access Journals (Sweden)

    Maria Angela Machado Fernandes

    2009-05-01

    Full Text Available O trabalho objetivou analisar a viabilidade econômica da produção de cordeiros para carne e identificar os componentes que exercem maior influência sobre o custo de produção. Este estudo foi realizado em duas etapas. Primeira etapa: experimento no Lapoc-UFPR, em 2004, que comparou a terminação de cordeiros para abate aos 32 kg: (1 desmame aos 40 dias e terminação em pasto, (2 cordeiro com mãe em pasto, (3 cordeiro com mãe em pasto e creep feeding (1% PV dia-1 de concentrado e (4 desmame aos 40 dias e confinamento. Segunda etapa: um módulo de 150 ovelhas foi projetado a partir do experimento para avaliações econômicas. Foram realizados cálculos de custo fixo, variável, total, lucratividade e rentabilidade, valor presente líquido, taxa interna de retorno e benefício:custo. O confinamento exigiu maior investimento inicial, sendo 21% maior que os sistemas em pasto. A margem líquida anual foi positiva em todos os sistemas, e o resultado econômico foi positivo apenas no sistema sem desmame. A mão-de-obra e a alimentação foram os maiores custos em todos os sistemas. A maior lucratividade foi observada com cordeiros terminados em pasto, sem desmame. Todos os sistemas apresentaram VPL negativo e TIR menor que 5% a.a. A terminação de cordeiros em pastagem sem desmame apresenta melhores resultados econômicos.This study evaluated economic return of lamb meat production and identified the compounds that have greater influence on production cost. The study was carried out in two stages. Stage 1: experiment at Lapoc-UFPR in 2004 to compare lamb finishing for slaughter at 32 kg: (1 40-days-old weaned lamb kept under pasture; (2 lamb with mother on pasture; (3 lamb with mother on pasture and concentrate (1% BW day-1 in creep feeding; and (4 40-days-old weaned lamb in feedlot. Stage 2: a module of 150 ewes was projected for economic evaluations considering meat and animals for breeding sale. Calculation of fixed, variable, total costs

  8. Concentrate supplementation in lambs finished at pasture on cost of production in the dry seasonSuplementação concentrada para cordeiros terminados a pasto sobre custo de produção no período da seca

    Directory of Open Access Journals (Sweden)

    Marciano Moreira da Silva

    2012-05-01

    Full Text Available The objective was evaluate the levels of concentrate supplementation (0.0%, 0.5%, 1.0% and 1.5% of body weight – BW consisting of cottonseed with ground corn (CSGC in proportion 50% each in lambs finished on pasture (Grass Marandu slaughtered at 31.7 kg BW, on average daily gain (ADG, days of supplementation (DS and cost of production in the dry season. We used 20 lambs with initial BW 19 ± 0.5 kg in four treatments. CSGC supplementation was provided in stalls separated by level of supplementation the eighteen hours daily. We used a completely randomized design to determine ADG and DS lambs supplemented with different levels of CSGC. The data of ADG and DS were evaluated by regression analysis considering 5% significance. The different levels of supplementation CSGC influenced quadratic (p Objetivou-se avaliar os níveis de suplementação concentrada (0,0%; 0,5%; 1,0% e 1,5% do peso corporal – PC constituída de caroço de algodão com grão de milho moído (CAGM na proporção de 50% cada, em cordeiros terminados a pasto (Capim Marandu abatidos com 31,7 kg de PC, sobre ganho médio diário (GMD, dias de suplementação (DS e custo de produção no período da seca. Foram utilizados 20 cordeiros sem raça definida com PC inicial de 19 ± 0,5 kg distribuídos em quatro tratamentos. A suplementação de CAGM foi fornecida em baias separadas por nível de suplementação diariamente as dezoito horas. Foi utilizado um delineamento inteiramente casualizado para avaliação do GMD e DS dos cordeiros suplementados com os diferentes níveis de CAGM. Os dados de GMD e DS foram avaliados por análise de regressão considerando 5 % de significância. Os diferentes níveis de suplementação de CAGM influenciaram de maneira quadrática (p < 0,05 o GMD e DS de cordeiros terminados a pasto. O nível de suplementação de CAGM a 1,0% do PC em cordeiros terminados a pasto no período da seca apresentou o melhor valor da receita total para carcaça quente

  9. In Vitro Activity of ABT-773 against Legionella pneumophila, Its Pharmacokinetics in Guinea Pigs, and Its Use to Treat Guinea Pigs with L. pneumophila Pneumonia

    OpenAIRE

    Edelstein, Paul H.; Higa, F.; Edelstein, Martha A. C.

    2001-01-01

    The activity of ABT-773 was studied against extracellular and intracellular Legionella pneumophila and for the treatment of guinea pigs with L. pneumophila pneumonia. The ABT-773 MIC at which 50% of isolates are inhibited (MIC50) for 20 different Legionella sp. strains was 0.016 μg/ml, whereas the MIC50s of clarithromycin and erythromycin were 0.032 and 0.125 μg/ml, respectively. ABT-773 (1 μg/ml) was bactericidal for two L. pneumophila strains grown in guinea pig alveolar macrophages. In con...

  10. La superaci??n del modelo del ??ngel del hogar:

    OpenAIRE

    Hurtado Mu??oz, M??nica

    2012-01-01

    La Tesis Doctoral titulada "La superaci??n del modelo del "??ngel del hogar". Recuperaci??n de la escritora Leonor Canalejas y Fustegueras (1869-1945)" aborda, en primer lugar, un an??lisis sociol??gico de las circunstancias que rodearon a la mujer espa??ola en el cambio de siglo en el ??mbito social y educativo, en un intento de esclarecer las caracter??sticas propias del contexto en el que la mujer accede a la intelectualidad en general y a la literatura en particular. Esta parte concluye q...

  11. Molecular cloning, sequence identification and expression profile of domestic guinea pig (Cavia porcellus UGT1A1 gene

    Directory of Open Access Journals (Sweden)

    Yang Deming

    2016-01-01

    Full Text Available Domestic guinea pig is a model animal for human disease research. Uridine diphosphate glucuronosyltransferase 1 family, polypeptide A1 (UGT1A1 is an important human disease-related gene. In this study, the complete coding sequence of domestic guinea pig gene UGT1A1 was amplified by reverse transcription-polymerase chain reaction. The open reading frame of the domestic guinea pig UGT1A1 gene is 1602 bp in length and was found to encode a protein of 533 amino acids. Sequence analysis revealed that the UGT1A1 protein of domestic guinea pig shared high homology with the UGT1A1 proteins of degu (84%, damara mole-rat (84%, human (80%, northern white-cheeked gibbon (80%, Colobus angolensis palliatus (80% and golden snub-nosed monkey (79%. This gene contains five exons and four introns, as revealed by the computer-assisted analysis. The results also showed that the domestic guinea pig UGT1A1 gene had a close genetic relationship with the UGT1A1 gene of degu. The prediction of transmembrane helices showed that domestic guinea pig UGT1A1 might be a transmembrane protein. Expression profile analysis indicated that the domestic guinea pig UGT1A1 gene was differentially expressed in detected domestic guinea pig tissues. Our experiment laid a primary foundation for using the domestic guinea pig as a model animal to study the UGT1A1-related human diseases.

  12. Urea levels in multiple supplement for lambs grazing on buffelgrass = Teores de uréia no suplemento múltiplo de cordeiros mantidos em pastos de capim-búffel

    Directory of Open Access Journals (Sweden)

    Pablo Leal Teixeira de Oliveira

    2010-10-01

    Full Text Available The objective of present trial was to evaluate the intake of supplement dry matter, productive performance, carcass traits, and weight and yields of commercial meat cuts of lambs grazing on dormant buffelgrass pastures and receiving multiple supplementation containing different urea levels. Urea levels used were: 5, 8, 11 and 14% on dry matter. Thirty-six male lambs were used, nine animals per treatment, with 18.0 �� 2.0 kg initial body weight, distributed into four groups. The experimental design was completely randomized with nine replications. Urea levels in supplement did not influence productive performance, cold and hot carcass weight and yield and commercial meat cuts weight and yield (leg, shoulder, rib and brisket. Use of multiple supplements containing 11 and 14% of urea levels for lambs in dormant grazing pastures during the dry season may increase bioeconomic performance of this activity, as they promoted lower supplement intake and similar productive performance.O objetivo do presente estudo foi avaliar o consumo de matéria seca de suplemento, o desempenho produtivo, os parâmetros de carcaça e os rendimentos de cortes cárneos comerciais de cordeiros mantidos em pastos diferidos de capim-búffel recebendo suplementos múltiplos com diferentes teores de uréia. Foram utilizados quatro teores de uréia, sendo 5, 8, 11 e 14% na matéria seca. Foram utilizados 36 cordeiros machos, nove animais por tratamento, com peso corporal inicial médio de 18,0 ± 2,0 kg, distribuídos em quatro grupos. O delineamento experimental adotado foi o inteiramente casualizado com nove repetições. Os teores de uréia no concentrado não influenciaram os ganhos médios diários, os ganhos de peso totais, os pesos corporais finais, os pesos e os rendimentos de carcaça quente e fria e os pesos e rendimentos de cortes cárneos comerciais (pernil, paleta, costela e carré. O fornecimento de suplementos múltiplos contendo 11 e 14% deuréia para ovinos

  13. EVALUACIÓN DEL RIESGO DE CONTAMINACIÓN POR METAMIDOFOS EN LA MICROCUENCA EL SALTO DEL MUNICIPIO DE EL SANTUARIO, ANTIOQUIA

    Directory of Open Access Journals (Sweden)

    David Horacio Ramírez

    2007-12-01

    Full Text Available En este trabajo de investigación, que se desarrolló en la microcuenca El Salto del municipio de El Santuario, se estimó la presencia del Metamidofos, en plantas de repollo (Brassica oleraceae y en el suelo y su riesgo de contaminación en aguas para el consumo humano. En el repollo el nivel de concentración del Metamidofos sobrepasó los límites máximos permisibles establecidos para esta planta por el Codex Alimentarius; en el suelo no se encontraron residuos del producto, pero sí en el agua de escorrentía y en dos sitios del cauce de la quebrada El Salto localizados aguas arriba de la bocatoma del acueducto municipal; no se detectó Metamidofos en la muestra de agua de la bocatoma del acueducto ni en la red de distribución del acueducto municipal. En la estimación cualitativa del riesgo de contaminación se utilizaron como variables de vulnerabilidad y amenaza la precipitación, la escorrentía, el coeficiente de escorrentía, el tiempo de concentración, la erosión, algunas propiedades físicas, químicas y biológicas del suelo y la pendiente de los terrenos. Se observó que el riesgo se presenta por la alta cantidad de insecticida utilizado y su frecuencia de utilización, que se incrementa según la distribución e intensidad de las lluvias y del potencial de erosión y este, a su vez, por el uso y manejo no adecuado del suelo. En la parcela experimental y en un terreno aledaño sembrado con pasto kikuyo (Pennisetum clandestinum, se calculó la población por unidad de área de la lombriz de tierra (Lumbricus terrestris. La ausencia de este organismo en la parcela cultivada con repollo (Brassica oleraceae se puede considerar como indicador biológico por la contaminación de este insecticida en el suelo.This investigation was done in El Salto watershed basin located in the municipality of El Santuario. It was examined the presence of Metamidofos in plants of cabbage, in soils, with risk of contamination in waters for the human

  14. Deterioration of epithelium mediated mechanisms in diabetic-antigen sensitized airways of guinea pigs.

    Science.gov (United States)

    Bano, Saidullah; Swati, Omanwar; Kambadur, Muralidhar; Mohammad, Fahim

    2016-01-01

    The onset of diabetes causes disruption of respiratory epithelial mediators. The present study investigates whether diabetes modifies the epithelium mediated bronchial responses in hyper-reactive airway smooth muscle (ASM) primarily through nitric oxide (NO), cyclooxygenase (COX), and epithelium derived hyperpolarizing factor (EpDHF) pathways. Experimental model of guinea pigs having hyper-reactive airways with or without diabetes were developed. The responses of tracheal rings to cumulative concentrations of acetylcholine (ACh) and isoproterenol (IP) in the presence and absence of epithelium and before and after incubation with NO, K + ATP and COX inhibitors, N-(ω)-Nitro-L-arginine methyl ester (L-NAME; 100 μM), glybenclamide (10 μM) and indomethacin (100 μM) were assessed. In diabetic guinea pigs with hyper-reactive airways, a decrease in ACh induced bronchoconstriction was observed after epithelium removal and after incubation with L-NAME/indomethacin, suggesting damage to NO/COX pathways. Hyper-reactivity did not alter the response of trachea to ACh but affected the response to IP which was further reduced in hyper-reactive animals with diabetes. The ASM response to IP after glybenclamide treatment did not alter in hyper-reactive guinea pigs and diabetic guinea pigs with hyper-reactive airways, suggesting damage to the EpDHF pathway. Treatment with indomethacin reduced IP response in the hyper-reactive model, and did not produce any change in diabetic model with hyper-reactive airways, indicating further disruption of the COX pathway. EpDHF pathway is damaged in hyper-reactive guinea pigs and in diabetic guinea pigs with hyper-reactive airways. Diabetes further aggravates the NO and COX mediated pathways in diabetic guinea pigs with hyper-reactive airways.

  15. [Effects of electroacupuncture on cochlea morphology and expression of aquaporins in guinea pigs with endolymphatic hydrops].

    Science.gov (United States)

    Jiang, Liyuan; Wang, Canjun; Ni, Fangying; Chen, Huade

    2015-06-01

    To observe the effects of electroacupuncture (EA) on cochlea morphology and expression of aquaporin 1 (AQP1) in guinea pigs with endolymphatic hydrops, so as to explore the possible mechanism of EA on endolymphatic hydrops. Forty guinea pigs were randomly divided into a blank group, a model group, a medication group and an EA group, 10 guinea pigs in each one. Model of endolymphatic hydrops was established by using intraperitoneal injection of aldosterone. Guinea pigs in the blank group and model group were treated with identical immobilization as EA group but no treatment was given; guinea pigs in the medication group were treated with intragastric administration of hydrochlorothiazide at a dose of 5 mg/kg, once a day for consecutive 10 days; guinea pigs in the EA group were treated with' EA at "Baihui" (GV 20) and "Tinggong"(SI 19), once a day for consecutive 10 days. The serum ionic concentration in each group was tested by turbidimetric method; hematoxylin-eosin staining was used to measure the severity of cochlea hydrops; immunohistochemical method was used to observe the expression of AQP1 in the cochlea. (1) There was no endolymphatic hydrops in the blank group, moderate-severe endolymphatic hydrops in the model group and slight endolymphatic hydrops in the EA group and medication group. (2) The concentration of K+ and Ca2+ in the EA group was higher than that in the model group and medication group (all P0. 05). (3) The ratio of expression area of AQP1 in the model group was lower than that in the blank group (P0. 05). EA could relieve the endolymphatic hydrops in guinea pigs; the mechanism is likely to be related with up-regulating the expression of AQP1 in cochlea and ion concentration might be an important factor involved.

  16. Airway hyperresponsiveness induced by repeated esophageal infusion of HCl in guinea pigs.

    Science.gov (United States)

    Cheng, Yan-Mei; Cao, Ai-Li; Zheng, Jian-Pu; Wang, Hong-Wei; Sun, Yong-Shun; Liu, Chun-Fang; Zhang, Bei-Bei; Wang, Yi; Zhu, Sheng-Liang; Wu, Da-Zheng

    2014-11-01

    Gastroesophageal reflux is a common disorder closely related to chronic airway diseases, such as chronic cough, asthma, chronic bronchitis, and chronic obstructive disease. Indeed, gastroesophageal acid reflux into the respiratory tract causes bronchoconstriction, but the underlying mechanisms have still not been clarified. This study aimed to elucidate functional changes of bronchial smooth muscles (BSMs) isolated from guinea pigs in an animal model of gastroesophageal reflux. The marked airway inflammation, hyperresponsiveness and remodeling were observed after guinea pigs were exposed to intraesophageal HCl infusion for 14 days. In addition, contractile responses to acetylcholine (ACh), KCl, electrical field stimulation, and extracellular Ca(2+) were greater in guinea pigs infused with HCl compared with control groups. The L-type voltage-dependent Ca(2+) channels (L-VDCC) blocker, nicardipine, significantly inhibited ACh- and Ca(2+)-enhanced BSM contractions in guinea pigs infused with HCl. The Rho-kinase inhibitor, Y27632, attenuated ACh-enhanced BSM contractions in guinea pigs infused with HCl. Moreover, mRNA and protein expressions for muscarinic M2 and M3 receptors, RhoA, and L-VDCC in BSM were detected by real-time PCR and Western blot. Expressions of mRNA and protein for muscarinic M3 receptors, RhoA, and L-VDCC were greater than in BSM of HCl-infused guinea pigs, whereas levels of muscarinic M2 receptors were unchanged. We demonstrate that acid infusion to the lower esophagus and, subsequently, microaspiration into the respiratory tract in guinea pigs leads to airway hyperresponsiveness and overactive BSM. Functional and molecular results indicate that overactive BSM is the reason for enhancement of extracellular Ca(2+) influx via L-VDCC and Ca(2+) sensitization through Rho-kinase signaling.

  17. Expression Profile of the Integrin Receptor Subunits in the Guinea Pig Sclera.

    Science.gov (United States)

    Wang, Kevin K; Metlapally, Ravikanth; Wildsoet, Christine F

    2017-06-01

    The ocular dimensional changes in myopia reflect increased scleral remodeling, and in high myopia, loss of scleral integrity leads to biomechanical weakening and continued scleral creep. As integrins, a type of cell surface receptors, have been linked to scleral remodeling, they represent potential targets for myopia therapies. As a first step, this study aimed to characterize the integrin subunits at the messenger RNA level in the sclera of the guinea pig, a more recently added but increasingly used animal model for myopia research. Primers for α and β integrin subunits were designed using NCBI/UCSC Genome Browser and Primer3 software tools. Total RNA was extracted from normal scleral tissue and isolated cultured scleral fibroblasts, as well as liver and lung, as reference tissues, all from guinea pig. cDNA was produced by reverse transcription, PCR was used to amplify products of predetermined sizes, and products were sequenced using standard methods. Guinea pig scleral tissue expressed all known integrin alpha subunits except αD and αE. The latter integrin subunits were also not expressed by cultured guinea pig scleral fibroblasts; however, their expression was confirmed in guinea pig liver. In addition, isolated cultured fibroblasts did not express integrin subunits αL, αM, and αX. This difference between results for cultured cells and intact sclera presumably reflects the presence in the latter of additional cell types. Both guinea pig scleral tissue and isolated scleral fibroblasts expressed all known integrin beta subunits. All results were verified through sequencing. The possible contributions of integrins to scleral remodeling make them plausible targets for myopia prevention. Data from this study will help guide future ex vivo and in vitro studies directed at understanding the relationship between scleral integrins and ocular growth regulation in the guinea pig model for myopia.

  18. Adrenaline reveals the torsadogenic effect of combined blockade of potassium channels in anaesthetized guinea pigs.

    Science.gov (United States)

    Michael, G; Kane, K A; Coker, S J

    2008-08-01

    Torsade de pointes (TdP) can be induced in several species by a reduction in cardiac repolarizing capacity. The aim of this study was to assess whether combined I(Kr) and I(Ks) blockade could induce TdP in anaesthetized guinea pigs and whether short-term variability (STV) or triangulation of action potentials could predict TdP. Experiments were performed in open-chest, pentobarbital-anaesthetized, adrenaline-stimulated male Dunkin Hartley guinea pigs, which received three consecutive i.v. infusions of either vehicle, the I(Kr) blocker E-4031 (3, 10 and 30 nmol kg(-1) min(-1)), the I(Ks) blocker HMR1556 (75, 250, 750 nmol kg(-1) min(-1)) or E-4031 and HMR1556 combined. Phenylephrine-stimulated guinea pigs were also treated with the K(+) channel blockers in combination. Arterial blood pressure, ECGs and epicardial monophasic action potential (MAP) were recorded. TdP was observed in 75% of adrenaline-stimulated guinea pigs given the K(+) channel blockers in combination, but was not observed in guinea pigs treated with either I(K) blocker alone, or in phenylephrine-stimulated guinea pigs. Salvos and ventricular tachycardia occurred with adrenaline but not with phenylephrine. No changes in STV or triangulation of the MAP signals were observed before TdP. Combined blockade of both I(Kr) and I(Ks) plus the addition of adrenaline were required to induce TdP in anaesthetized guinea pigs. This suggests that there must be sufficient depletion of repolarization reserve and an appropriate trigger for TdP to occur.

  19. "Training by Papua New Guinea Women, for Papua New Guinea Women": Lessons from the Development of a Co-Constructed Course for Women Smallholder Farmers

    Science.gov (United States)

    Pamphilon, Barbara; Mikhailovich, Katja; Chambers, Barbara

    2014-01-01

    This article examines the lessons from a collaborative project that worked with women agricultural leaders in Papua New Guinea. The project sought to build the capacity of these leaders as trainers in a way that would enable the development of a sustainable community of practice and worked within a critical and place-based pedagogy underpinned by…

  20. Health promotion and empowerment in Henganofi District, Papua New Guinea.

    Science.gov (United States)

    Barcham, Richard; Silas, Esther; Irie, Jesse

    2016-01-01

    Evidence shows that the government of Papua New Guinea is failing to provide basic services in health to the majority of its people. Local non-government organisations (NGOs), partnered with international NGOs, are attempting to fill this gap. With limited resources, these small Indigenous organisations must focus much of their effort on training that supports self-reliance as the main strategy for communities to improve their quality of life. This project explored the training content and methodology of Touching The Untouchables (TTU), a small Indigenous NGO based in Goroka, Eastern Highlands Province, that has trained a network of village volunteers in health promotion and safe motherhood.Village life imposes multiple demands, from self-sufficiency in food to maintaining law and order. There are established attitudes about power and dependence, referred to as 'cargo thinking'. Cargo thinking stands as a barrier to the necessity of self-reliance, and requires training strategies that seek to empower participants to create change from their own initiative. Empowerment is understood as oriented towards individual people taking collective action to improve their circumstances by rectifying disparities in social power and control. To achieve self-reliance, empowerment is necessarily operational on the levels of person, community and society.In addition to being operational on all three levels of empowerment, the training content and methodology adopted and developed by TTU demonstrate that empowering practice in training employs approaches to knowledge that are evidence-based, reflexive, contextual and skill-based. Creating knowledge that is reflexive and exploring knowledge about the broader context uses special kinds of communicative tools that facilitate discussion on history, society and political economy. Furthermore, training methodologies that are oriented to empowerment create settings that require the use of all three types of communication required for