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Sample records for def stan 00-18

  1. Kuiper Prize Lecture: Stan Peale's Legacy

    Science.gov (United States)

    Margot, Jean-Luc

    2016-10-01

    Stan Peale's career in planetary science spanned over five decades and yielded an impressive record of high-impact results. His contributions include the prediction of widespread volcanism on Jupiter's moon Io, the derivation of a general theoretical framework that governs the rotational states of bodies subject to tides, the study of the origin and evolution of natural satellites, advances in our understanding of exoplanet dynamics, and the promotion of microlensing searches for exoplanets. Stan also developed an ingenious procedure to determine the size and state of Mercury's core. Because of this work, we know more about the core of Mercury than that of any planet other than Earth. Stan left us an enduring legacy that exemplifies the power of physics to probe the interiors of planets.

  2. Stan Z. Li: Rolling face recognition

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Among the galaxy of research and development organizations in northwest Beijing's Zhongguancun district, known as China's Silicon Valley, two are especially well-known to IT researchers: Microsoft Research Asia (MSRA), and, within a few blocks on the other side of the street, the CAS Institute of Automation (CASIA). While most people in Chinese scientific organizations strive to move to a large foreign counterpart, Dr. Stan Z. Li (LI Ziqing) moves against the stream.

  3. Stan: A Probabilistic Programming Language for Bayesian Inference and Optimization

    Science.gov (United States)

    Gelman, Andrew; Lee, Daniel; Guo, Jiqiang

    2015-01-01

    Stan is a free and open-source C++ program that performs Bayesian inference or optimization for arbitrary user-specified models and can be called from the command line, R, Python, Matlab, or Julia and has great promise for fitting large and complex statistical models in many areas of application. We discuss Stan from users' and developers'…

  4. Efficient Implementation of the Plan Graph in STAN

    CERN Document Server

    Fox, M; 10.1613/jair.570

    2011-01-01

    STAN is a Graphplan-based planner, so-called because it uses a variety of STate ANalysis techniques to enhance its performance. STAN competed in the AIPS-98 planning competition where it compared well with the other competitors in terms of speed, finding solutions fastest to many of the problems posed. Although the domain analysis techniques STAN exploits are an important factor in its overall performance, we believe that the speed at which STAN solved the competition problems is largely due to the implementation of its plan graph. The implementation is based on two insights: that many of the graph construction operations can be implemented as bit-level logical operations on bit vectors, and that the graph should not be explicitly constructed beyond the fix point. This paper describes the implementation of STAN's plan graph and provides experimental results which demonstrate the circumstances under which advantages can be obtained from using this implementation.

  5. DEF: The Physical Basis of Electromagnetic Propulsion

    CERN Document Server

    Pinheiro, Mario J

    2015-01-01

    The very existence of the physical vacuum provides a framework to propose a general mechanism for propelling bodies through an agency of electromagnetic fields, that seat in that medium. When two sub-systems of a general closed device interact via nonlocal and retarded electromagnetic pulses, it is easily shown that they give a nonzero force, and that only tend to comply with the action-to-reaction force in the limit of instantaneous interactions. The arrangement of sub-systems provide a handy way to optimize the unbalanced EM force with the concept of impedance matching. The general properties of the differential electromagnetic force (DEF) are the following: i) it is proportional to the square of the intensity and to the angular wave frequency $\\omega$; ii) to the space between the sub-systems (although in a non-linear manner); iii) it is inversely proportional to the speed of interaction; iv) when the two sub-systems are out-of-phase, DEF is null. The approach is of interest to practical engineering princi...

  6. Phosphorylation of Def Regulates Nucleolar p53 Turnover and Cell Cycle Progression through Def Recruitment of Calpain3

    Science.gov (United States)

    Tao, Ting; Shi, Hui; Lo, Li Jan; Wang, Yingchun; Chen, Jun; Peng, Jinrong

    2016-01-01

    Digestive organ expansion factor (Def) is a nucleolar protein that plays dual functions: it serves as a component of the ribosomal small subunit processome for the biogenesis of ribosomes and also mediates p53 degradation through the cysteine proteinase calpain-3 (CAPN3). However, nothing is known about the exact relationship between Def and CAPN3 or the regulation of the Def function. In this report, we show that CAPN3 degrades p53 and its mutant proteins p53A138V, p53M237I, p53R248W, and p53R273P but not the p53R175H mutant protein. Importantly, we show that Def directly interacts with CAPN3 in the nucleoli and determines the nucleolar localisation of CAPN3, which is a prerequisite for the degradation of p53 in the nucleolus. Furthermore, we find that Def is modified by phosphorylation at five serine residues: S50, S58, S62, S87, and S92. We further show that simultaneous phosphorylations at S87 and S92 facilitate the nucleolar localisation of Capn3 that is not only essential for the degradation of p53 but is also important for regulating cell cycle progression. Hence, we propose that the Def-CAPN3 pathway serves as a nucleolar checkpoint for cell proliferation by selective inactivation of cell cycle-related substrates during organogenesis. PMID:27657329

  7. Regulatory Regions of smeDEF in Stenotrophomonas maltophilia Strains Expressing Different Amounts of the Multidrug Efflux Pump SmeDEF

    OpenAIRE

    Sanchez, Patricia; Alonso, Ana; Martinez, Jose L.

    2004-01-01

    The smeT-smeDEF region and the smeT gene, which encodes the smeDEF repressor, are highly polymorphic. Few changes in smeT might be associated with smeDEF overexpression. The results obtained with cellular extracts suggest that mutant SmeT proteins cannot bind to the operator and that other transcription factors besides SmeT are involved in the regulation of smeDEF expression.

  8. GOODFOOT HUF DQM UNDEFEATED ADIDAS STAN SMITH 五家联手

    Institute of Scientific and Technical Information of China (English)

    米粉

    2009-01-01

    这次Goodfoot、HUF、DQM、Undefeated四大北美球鞋名所与adidas合作设计了Stan Smith。这款Consortium别注Stan Smith 80s就采用了多年前高帮Stan Smith的灰色翻毛皮风格制作,全无花哨的米色单色基调.只可惜鞋舌还是撤销了Stan Smith的招牌胡须人头像,虽然略有遗憾.但仍然很有看头。

  9. PhishDef: URL Names Say It All

    CERN Document Server

    Le, Anh; Faloutsos, Michalis

    2010-01-01

    Phishing is an increasingly sophisticated method to steal personal user information using sites that pretend to be legitimate. In this paper, we take the following steps to identify phishing URLs. First, we carefully select lexical features of the URLs that are resistant to obfuscation techniques used by attackers. Second, we evaluate the classification accuracy when using only lexical features, both automatically and hand-selected, vs. when using additional features. We show that lexical features are sufficient for all practical purposes. Third, we thoroughly compare several classification algorithms, and we propose to use an online method (AROW) that is able to overcome noisy training data. Based on the insights gained from our analysis, we propose PhishDef, a phishing detection system that uses only URL names and combines the above three elements. PhishDef is a highly accurate method (when compared to state-of-the-art approaches over real datasets), lightweight (thus appropriate for online and client-side ...

  10. Study of the DEF Feedback Control System in AC Operation of Superconducting Tokamak

    Institute of Scientific and Technical Information of China (English)

    WANG Hua; LUO Jiarong; YUAN Qiping; XU Congdong

    2007-01-01

    AC operation with multiple full cycles has been successfully performed on the superconducting tokamak HT-7. In the experiment, it was discovered that the saturation of the transformer magnetic flux with DEF, a signal name, was one of key aspects that affected the AC operation. The conditions of DEF were examined through the DEF feedback control system. By controlling the working patterns of the subsystems, namely the poloidal field control system and density control system, it was guaranteed that DEF would remain in the non-saturated status.

  11. Development of new auxiliary basis functions of the Karlsruhe segmented contracted basis sets including diffuse basis functions (def2-SVPD, def2-TZVPPD, and def2-QVPPD) for RI-MP2 and RI-CC calculations.

    Science.gov (United States)

    Hellweg, Arnim; Rappoport, Dmitrij

    2015-01-14

    We report optimized auxiliary basis sets for use with the Karlsruhe segmented contracted basis sets including moderately diffuse basis functions (Rappoport and Furche, J. Chem. Phys., 2010, 133, 134105) in resolution-of-the-identity (RI) post-self-consistent field (post-SCF) computations for the elements H-Rn (except lanthanides). The errors of the RI approximation using optimized auxiliary basis sets are analyzed on a comprehensive test set of molecules containing the most common oxidation states of each element and do not exceed those of the corresponding unaugmented basis sets. During these studies an unsatisfying performance of the def2-SVP and def2-QZVPP auxiliary basis sets for Barium was found and improved sets are provided. We establish the versatility of the def2-SVPD, def2-TZVPPD, and def2-QZVPPD basis sets for RI-MP2 and RI-CC (coupled-cluster) energy and property calculations. The influence of diffuse basis functions on correlation energy, basis set superposition error, atomic electron affinity, dipole moments, and computational timings is evaluated at different levels of theory using benchmark sets and showcase examples.

  12. Haploinsufficiency of Def activates p53-dependent TGFβ signalling and causes scar formation after partial hepatectomy.

    Directory of Open Access Journals (Sweden)

    Zhihui Zhu

    Full Text Available The metazoan liver exhibits a remarkable capacity to regenerate lost liver mass without leaving a scar following partial hepatectomy (PH. Whilst previous studies have identified components of several different signaling pathways that are essential for activation of hepatocyte proliferation during liver regeneration, the mechanisms that enable such regeneration to occur without accompanying scar formation remain poorly understood. Here we use the adult zebrafish liver, which can regenerate within two weeks following PH, as a new genetic model to address this important question. We focus on the role of Digestive-organ-expansion-factor (Def, a nucleolar protein which has recently been shown to complex with calpain3 (Capn3 to mediate p53 degradation specifically in the nucleolus, in liver regeneration. Firstly, we show that Def expression is up-regulated in the wild-type liver following amputation, and that the defhi429/+ heteroozygous mutant (def+/- suffers from haploinsufficiency of Def in the liver. We then show that the expression of pro-inflammatory cytokines is up-regulated in the def+/- liver, which leads to distortion of the migration and the clearance of leukocytes after PH. Transforming growth factor β (TGFβ signalling is thus activated in the wound epidermis in def+/- due to a prolonged inflammatory response, which leads to fibrosis at the amputation site. Fibrotic scar formation in def+/- is blocked by the over-expression of Def, by the loss-of-function of p53, and by treatment with anti-inflammation drug dexamethasone or TGFβ-signalling inhibitor SB431542. We finally show that the Def- p53 pathway suppresses fibrotic scar formation, at least in part, through the regulation of the expression of the pro-inflammatory factor, high-mobility group box 1. We conclude that the novel Def- p53 nucleolar pathway functions specifically to prevent a scar formation at the amputation site in a normal amputated liver.

  13. Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L

    Directory of Open Access Journals (Sweden)

    Ayeh Kwadwo

    2011-11-01

    Full Text Available Abstract Background The def mutant pea (Pisum sativum L showed non-abscission of seeds from the funicule. Here we present data on seed development and growth pattern and their relationship in predicting this particular trait in wild type and mutant lines as well as the inheritance pattern of the def allele in F2 and F3 populations. Findings Pod length and seed fresh weight increase with fruit maturity and this may affect the abscission event in pea seeds. However, the seed position in either the distal and proximal ends of the pod did not show any difference. The growth factors of seed fresh weight (FW, width of funicles (WFN, seed width (SW and seed height (SH were highly correlated and their relationships were determined in both wild type and def mutant peas. The coefficient of determination R2 values for the relationship between WFN and FW, SW and SH and their various interactions were higher for the def dwarf type. Stepwise multiple regression analysis showed that variation of WFN was associated with SH and SW. Pearson's chi square analysis revealed that the inheritance and segregation of the Def locus in 3:1 ratio was significant in two F2 populations. Structural analysis of the F3 population was used to confirm the inheritance status of the Def locus in F2 heterozygote plants. Conclusions This study investigated the inheritance of the presence or absence of the Def allele, controlling the presence of an abscission zone (AZ or an abscission-less zone (ALZ forming in wild type and mutant lines respectively. The single major gene (Def controlling this phenotype was monogenic and def mutants were characterized and controlled by the homozygous recessive def allele that showed no palisade layers in the hilum region of the seed coat.

  14. In Vitro Anti-arthritic activity of Tecoma stans (Linn. Leaves

    Directory of Open Access Journals (Sweden)

    Dharmeshkumar Prajapati

    2015-04-01

    Full Text Available The World Health Organization (WHO estimates 80% of the world population presently use herbal medicine for some aspects of primary health care. Rheumatoid arthritis (RA is a chronic, systemic inflammatory disorder that may affect many tissues and organs, but principally attacks synovial joints. There are many herbs which are described in Ayurveda for arthritis. The medicinal plant contains flavonoids, terpenes, quinones, catechins, alkaloids, anthocyanins and anthoxanthins phytoconstituents, having anti-inflammatory effects. Tecoma stans (Linn. belongs to the family of Bignoniaceae. The root of Tecoma stans as diuretic, vermifuge and tonic. Pharmacological reports revealed that it is having antidiabetic, anticancer, antioxidant, anti-inflammatory activity, antimicrobial, and antifungal properties, and extensively used in the treatment of diabetes. Alcohol, Water, successive extracts of Petroleum ether, Chloroform, Methanol and Water extracts of T. stans leaves were tested for Antiarthritic activity using Diclofenac sodium as standard by In-vitro models like, Inhibition of protein denaturation and effect on membrane stabilization. Alcohol, Water and succesive Methanol extracts of T. stans leaves exhibited significant Antiarthritic activity. The results of the investigations justify us the folklore use of T. stans leaves in the treatment of inflammation during arthritis and the plant is worth for further chemical isolation and pharmacological investigations.

  15. [Bee diversity in Tecoma stans (L.) Kunth (Bignoniaceae): importance for pollination and fruit production].

    Science.gov (United States)

    Silva, Cláudia I; Augusto, Solange C; Sofia, Silvia H; Moscheta, Ismar S

    2007-01-01

    Tecoma stans (L.) Kunth is an exotic plant in Brazil, commonly distributed in urban areas, which is considered an invasive species in crop and pasture areas. In this study, the floral biology and the behavior of bees in flowers of T. stans from three urban areas in southeastern Brazil were investigated. In all study sites, T. stans was an important food resource to the Apoidea to 48 species of bees. Centris tarsata Smith and Exomalopsis fulvofasciata Smith (Hymenoptera: Apidae) were the effective pollinators more abundant, while Scaptotrigona depilis Moure (Hymenoptera: Apidae) was the more frequent robber species. The most part of T. stans visitors (87.5%) exploited exclusively nectar, which varied in sugar concentration depending on the day period and flower phase. In all flower stages, higher averages of nectar concentration (26.4% to 32.7%) occurred from 10 am to 2 pm. The presence of osmophore in the petals and protandry were detected. In two urban areas the number of visitors varied significantly during the day. The greatest abundance of pollinators occurred when pollen availability was higher and flowers showed receptive stigma, which could be contributing to the reproductive success of T. stans. The results indicate that the production of fruits increased in plants that received a higher number of effective pollinators.

  16. Evaluasi Sistem Informasi Akuntansi Pendapatan dan Persediaan Pada PT. DEF

    Directory of Open Access Journals (Sweden)

    Kriswanto Kriswanto

    2012-11-01

    Full Text Available PT. DEF is a company engaged in the trading and distribution of food and frozen vegetables to customers in several restaurants and hotels in the big cities in Java. The problems faced by the company based on the results of observations made relating to revenue and inventory due to lack of internal controls in the company are because of the duplication of functions between the accounting and finance, many expired items should be disposed of and affect the company's earnings, collection of accounts receivable which resulted to financial turnaround interrupted, the data inventory is not accurate and complete so that the company's customer service to be less than satisfactory, and the problem of delay in making financial statements. The research method used in this study is data collection method which includes literature and field studies, analysis of findings, identifying the problems and the needs of information, and analysis method on object-oriented approach. The revenue accounting information systems and computerized inventory are expected to help the company run its operations, implement of internal controls within the company better, and overcome all the problems.

  17. Stan's Cafe tõi publiku näole laia naeratuse / Holger Roonemaa

    Index Scriptorium Estoniae

    Roonemaa, Holger

    2002-01-01

    Rakvere seitsmenda rahvusvahelise teatrifestivali Baltoscandal üks omapärasemaid esinejaid oli Birminghamist pärit teatrikompanii Stan's Cafe, mis mängis oma etendust "See on sinu film" (It's Your Film") korraga ainult ühele vaatajale

  18. Cardioprotective effect of hydroalcoholic extract of Tecoma stans flowers against isoproterenol induced myocardial infarction in rats

    Directory of Open Access Journals (Sweden)

    Shanmukha Ittagi

    2014-02-01

    Full Text Available Objective: To investigate the cardioprotective effect of 70% ethanolic extract of Tecoma stans (T. stans flowers against isoproterenol-induced myocardial infarction in rat myocardium. Methods: Wister rats were pretreated with 70% ethanolic extract of T. stans flowers (250 and 500 mg/kg orally for 14 d and then intoxicated with isoproterenol [200 mg/(kg · day, s.c.] for 2 consecutive d. The biochemical markers for myocardial infarction such as alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatinine kinase, total cholesterol, triglycerides, low density lipoproteins and high density lipoproteins were determined. In addition the antioxidant status on heart tissue is also evaluated by testing the activities of antioxidant enzymes such as lipid peroxidation, superoxide dismutase, reduced glutathione and catalase. Results: The results indicated that pretreatment with 70% ethanolic extract of T. stans flowers prevented fall in antioxidants and retarded elevation of cardiac damage markers in isoproterenol treated rats, significantly. In addition, these findings were evidently supported by the remarkable protection revealed in the histopathological studies, even GC-MS analysis data also substantiated out investigation. Conclusions: It was concluded that, in addition to poly phenolics, some of the phyto fragments found during GC-MS analysis might also contributed to the cardiac protection offered by the extract.

  19. Stan's Cafe tõi publiku näole laia naeratuse / Holger Roonemaa

    Index Scriptorium Estoniae

    Roonemaa, Holger

    2002-01-01

    Rakvere seitsmenda rahvusvahelise teatrifestivali Baltoscandal üks omapärasemaid esinejaid oli Birminghamist pärit teatrikompanii Stan's Cafe, mis mängis oma etendust "See on sinu film" (It's Your Film") korraga ainult ühele vaatajale

  20. Proteasome-Mediated Processing of Def1, a Critical Step in the Cellular Response to Transcription Stress

    OpenAIRE

    Wilson, Marcus D.; Harreman, Michelle; Taschner, Michael; Reid, James; Walker, Jane; Erdjument-Bromage, Hediye; Tempst, Paul; Svejstrup, Jesper Q.

    2013-01-01

    Summary DNA damage triggers polyubiquitylation and degradation of the largest subunit of RNA polymerase II (RNAPII), a “mechanism of last resort” employed during transcription stress. In yeast, this process is dependent on Def1 through a previously unresolved mechanism. Here, we report that Def1 becomes activated through ubiquitylation- and proteasome-dependent processing. Def1 processing results in the removal of a domain promoting cytoplasmic localization, resulting in nuclear accumulation ...

  1. An update of the DEF database of protein fold class predictions

    DEFF Research Database (Denmark)

    Reczko, Martin; Karras, Dimitris; Bohr, Henrik

    1997-01-01

    An update is given on the Database of Expected Fold classes (DEF) that contains a collection of fold-class predictions made from protein sequences and a mail server that provides new predictions for new sequences. To any given sequence one of 49 fold-classes is chosen to classify the structure...

  2. Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation

    Institute of Scientific and Technical Information of China (English)

    Ting Tao; Hui Shi; Yihong Guan; Delai Huang; Ye Chen; David P Lane; Jun Chen

    2013-01-01

    p53 protein turnover through the ubiquitination pathway is a vital mechanism in the regulation of its transcriptional activity; however,little is known about p53 turnover through proteasome-independent pathway(s).The digestive organ expansion factor (Def) protein is essential for the development of digestive organs.In zebrafish,loss of function of defselectively upregulates the expression of p53 response genes,which raises a question as to what is the relationship between Def and p53.We report here that Def is a nucleolar protein and that loss of function of defleads to the upregulation of p53 protein,which surprisingly accumulates in the nucleoli.Our extensive studies have demonstrated that Def can mediate the degradation of p53 protein and that this process is independent of the proteasome pathway,but dependent on the activity of Calpain3,a cysteine protease.Our findings define a novel nucleolar pathway that regulates the turnover function of p53,which will advance our understanding of p53's role in organogenesis and tumorigenesis.

  3. Identification of AFLP and STS markers closely linked to the def locus in pea.

    Science.gov (United States)

    von Stackelberg, M; Lindemann, S; Menke, M; Riesselmann, S; Jacobsen, H-J

    2003-05-01

    The recessive mutation of the def gene of pea (Pisum sativum L.) leads to the loss of the hilum, the abscission zone between the seed and the pod. Thereby, it reduces the free dispersal of the seeds through pod shattering. As a prerequisite for a gene isolation via a map-based cloning approach, bulked segregant analysis followed by single plant analyses of over 200 homozygous individuals of a population of 476 F2 plants derived from a cross between 'DGV' (def wild-type) and 'PF' (def mutant), were used to detect markers closely linked to the def locus. The AFLP technique in combination with silver staining was used to maximize numbers of reproducible marker loci. Fifteen AFLP loci showed a genetic distance less than 5 and two of them less than 1 centiMorgans (cM) to the gene of interest. AFLPs were converted into sequence tagged sites (STSs) and into a newly refined AFLP-based single locus marker named the 'sequence specified AFLP' (ssAFLP).

  4. X-ray diffraction analysis of stan nite, wurtz-stan nite and pseudo-cubic quaternary compounds by Rietveld method

    Energy Technology Data Exchange (ETDEWEB)

    Quintero, M.; Quintero, E.; Moreno, E.; Alvarez, S.; Rincon, C.; Grima, P.; Bocaranda, P.; Rivero, D. [Universidad de Los Andes, Centro de Estudios de Semiconductores, 5101 Merida (Venezuela, Bolivarian Republic of); Marquina, J. [Universidad de Los Andes, Centro de Estudios Avanzados en Optica, 5101 Merida (Venezuela, Bolivarian Republic of); Henao, J. A.; Macias, M. A., E-mail: mquinterg@gmail.com [Universidad Industrial de Santander, Facultad de Ciencias, Escuela de Quimica, Grupo de Investigacion en Quimica Estructural, Apdo. Aereo 678, Bucaramanga (Colombia)

    2014-07-01

    Room temperature X-ray powder diffraction measurements were carried out on nine polycrystalline samples of the Cu{sub 2}B{sup II}C{sup IV}X{sub 4} (B=Mn, or Fe, or Co; C=Si, or Ge, or Sn; X=S, or Se or Te) magnetic semiconductor compounds. The diffraction patterns were used to show the equilibrium conditions and to derive crystalline parameters values. The results showed that four of these compounds have a tetragonal stan nite structure with space group 142 m(N{sup o} 121), two and orthorhombic wurtz-stan nite structure with space group Pmn2{sub 1}(N{sup o} 31) and three of them and orthorhombic pseudo-cubic structure with space group F222 (N{sup o} 22). In each case, the structure was refined using the Rietveld method. When the obtained atomic parameter values for the tetragonal compounds were plotted as a function of molecular weight W, it was found that the values of the atomic positions, the cation-anion bond distances, tetragonal distortion and internal distortion of the compounds containing S and /or Se lay on different lines. Also, it was found that when the experimental points of the cation-anion bond distances d{sub Cu-VI}, d{sub II-VI} and d{sub IV-VI} were plotted against the effective lattice parameter a{sub c} = (V/N){sup 1/3}, a linear variation of these distances with a{sub c} was obtained. Values of the ionic energy gap C{sub i} and homopolar energy gap E{sub h} using the Phillips-Van Vechten scheme, with the present experimental crystallographic results as well as using the atomic data, were determined. It was found that the observed and predicted values of C{sub i} and E{sub h} lie on the same straight line. (Author)

  5. Orthogonal 4,10 and 6,12 substitution of dibenzo[def,mno]chrysene polycyclic aromatic small molecules

    KAUST Repository

    Koldemir, Unsal

    2017-07-14

    A series of new polycyclic aromatic hydrocarbon compounds based on (4,10-disubstituted-dibenzo[def,mno]chrysene-6,12-dione) and 4,10 di-substituted 6,12-bis(triisopropylsilylethynyl)dibenzo[def,mno]chrysene are reported with tunable electronic properties through varied molecular architecture. Starting with an inexpensive commercially available textile dye known as Vat Orange #3, (4,10-dibromo-dibenzo[def,mno] chrysene-6,12-dione) we extended the conjugation at the 4- and 10-positions by the attachment of both electron rich and deficient hexylvinylphthalimide, thiophene, hexylthiophene, triphenylamine, and hexylbithiophene aromatic groups, and studied the resultant optoelectronic properties. By applying various synthetic metal-catalyzed reactions, soluble dibenzo[def,mno]chrysene and dibenzo[def,mno]chrysene-6,12-dione derivatives were achieved with optical edge band gaps between 2.30 eV and 1.65 eV.

  6. Platelet receptors and patient responses: The contributions of Professor Stan Heptinstall to platelet research.

    Science.gov (United States)

    Clemetson, Kenneth J

    2015-01-01

    Stan Heptinstall's contributions to platelet research covered organising meetings at the national and European level as well as starting and maintaining the journal "Platelets". The major part of his research addressed problems of inhibition of platelet receptors and the effects of this on patient health. In particular, the effects of P2Y12 inhibitors on patients with acute cardiovascular problems were a major focus. Other studies included the effects of feverfew (Tanacetum parthenium) extracts on platelets, of direct anti-IIb/IIIa receptor (αIIbβ3) inhibitors and of prostanoids on platelet function. Recently, methods for assessing the effectiveness of platelet inhibition were investigated.

  7. Procesos educativos y redes solidarias en comunidades devastadas por el huracán Stan

    OpenAIRE

    Ramírez Reyes, Neptali

    2008-01-01

    Este documento describe las experiencias vividas durante una brigada de apoyo solidario a las Comunidades Chiapanecas devastadas por el huracán Stan durante el mes de diciembre 2005. La brigada “Tortuga” fue construida de manera espontánea por un grupo de estudiantes de licenciatura, quienes con el apoyo de diversos profesores de la Universidad Iberoamericana-Puebla recibieron entrenamiento y soporte para responder al llamado de diversas organizaciones de la región afectada. El documento alte...

  8. Interactions between insect pollinators and the ornamental tree, Tecoma stans (L.

    Directory of Open Access Journals (Sweden)

    K.H. Jonathan

    2009-02-01

    Full Text Available Tecoma stans (Bignoniaceae is a native of Central America but occurs throughout tropical latitudes due to cultivation as an ornamental; in India it is also normally cultivated as an ornamental tree. The plant has two anthesis schedules in a day - one during 0500-0800hrs and another during 1500-1700hrs. Different species of bees and wasps collect pollen and nectar from the flowers during daylight hours, while hawk moths collect nectar during the 0530-0700hrs and 1630-1830hrs periods. The flowers are an important source of pollen and/or nectar for these insects, and all facilitate pollination. The nectar contains three common sugars: hexoses, glucose and fructose, and it is also a source of three essential amino acids: lysine, histidine and threonine, and eight non-essential amino acids: glycine, serine, proline, arginine, glutamic acid, cystine, cysteine and alanine. With these floral rewards, T. stans sustains pollinator populations in areas where it is cultivated.

  9. Application of fluorocarbon remover DEF%氟树脂去除剂DEF的应用

    Institute of Scientific and Technical Information of China (English)

    董玲

    2012-01-01

    The fluorochemical water- and oil- repeffent on textiles must be stripped firstly in color restoration of finished polyester dyeings. The stripping process is carried out by immersion method with fluorocarbon remover DEF. Influences of remover dosage, NaOH dosage, stripping temperature and time on the stripping effects are investigated according to the water- and oil- repellent effect of polyester fabric before and after washing. The optimum stripping process is as follows; fluorocarbon remover DEF 1.0~1.5 g/L,Na0H 1 g/L, material ratio 1:20, stripping at 90~100 ℃ for 20 min. The results show that fluorocarbon re-mover DEF features good stripping effect. The water repellent effect of refinished fabric reaches 100 and the oil-repellent effect is 5. The refinished fabric has a little change in K/S value and a little decrease in tear strength.%含氟拒水拒油整理涤纶染色布修色时,需先剥除拒水拒油整理剂.试验采用氟树脂去除剂DEF,以浸渍法剥除织物上的整理剂.根据水洗前后织物的拒水拒油性能,讨论了去除剂用量、烧碱用量、温度、时间对剥除效果的影响,优化的剥除工艺为:去除剂DEF1.0-1.5 g/L,烧碱1 g/L,浴比1:20,温度90-100℃,时间20 min.结果表明,去除剂DEF可有效地剥除含氟整理剂,重新整理后织物拒水等级达到100分,拒油级别达到5级,对色光影响较小,强力稍有下降.

  10. A function of SmeDEF, the major quinolone resistance determinant of Stenotrophomonas maltophilia, is the colonization of plant roots.

    Science.gov (United States)

    García-León, Guillermo; Hernández, Alvaro; Hernando-Amado, Sara; Alavi, Peyman; Berg, Gabriele; Martínez, José Luis

    2014-08-01

    Quinolones are synthetic antibiotics, and the main cause of resistance to these antimicrobials is mutation of the genes encoding their targets. However, in contrast to the case for other organisms, such mutations have not been found in quinolone-resistant Stenotrophomonas maltophilia isolates, in which overproduction of the SmeDEF efflux pump is a major cause of quinolone resistance. SmeDEF is chromosomally encoded and highly conserved in all studied S. maltophilia strains; it is an ancient element that evolved over millions of years in this species. It thus seems unlikely that its main function would be resistance to quinolones, a family of synthetic antibiotics not present in natural environments until the last few decades. Expression of SmeDEF is tightly controlled by the transcriptional repressor SmeT. Our work shows that plant-produced flavonoids can bind to SmeT, releasing it from smeDEF and smeT operators. Antibiotics extruded by SmeDEF do not impede the binding of SmeT to DNA. The fact that plant-produced flavonoids specifically induce smeDEF expression indicates that they are bona fide effectors regulating expression of this resistance determinant. Expression of efflux pumps is usually downregulated unless their activity is needed. Since smeDEF expression is triggered by plant-produced flavonoids, we reasoned that this efflux pump may have a role in the colonization of plants by S. maltophilia. Our results showed that, indeed, deletion of smeE impairs S. maltophilia colonization of plant roots. Altogether, our results indicate that quinolone resistance is a recent function of SmeDEF and that colonization of plant roots is likely one original function of this efflux pump.

  11. Accurate Promoter and Enhancer Identification in 127 ENCODE and Roadmap Epigenomics Cell Types and Tissues by GenoSTAN

    Science.gov (United States)

    Zacher, Benedikt; Michel, Margaux; Schwalb, Björn; Cramer, Patrick; Tresch, Achim

    2017-01-01

    Accurate maps of promoters and enhancers are required for understanding transcriptional regulation. Promoters and enhancers are usually mapped by integration of chromatin assays charting histone modifications, DNA accessibility, and transcription factor binding. However, current algorithms are limited by unrealistic data distribution assumptions. Here we propose GenoSTAN (Genomic STate ANnotation), a hidden Markov model overcoming these limitations. We map promoters and enhancers for 127 cell types and tissues from the ENCODE and Roadmap Epigenomics projects, today’s largest compendium of chromatin assays. Extensive benchmarks demonstrate that GenoSTAN generally identifies promoters and enhancers with significantly higher accuracy than previous methods. Moreover, GenoSTAN-derived promoters and enhancers showed significantly higher enrichment of complex trait-associated genetic variants than current annotations. Altogether, GenoSTAN provides an easy-to-use tool to define promoters and enhancers in any system, and our annotation of human transcriptional cis-regulatory elements constitutes a rich resource for future research in biology and medicine. PMID:28056037

  12. Avaliação da fitotoxidez de Tecoma stans (L. Kunth

    Directory of Open Access Journals (Sweden)

    Franciane Auxiliadora Cipriani

    Full Text Available Tecoma stans foi introduzida no Brasil para fins ornamentais e ocorre tanto cultivada quanto subespontânea. Objetivando avaliar seu potencial alelopático, foi testado o efeito do extrato aquoso de suas folhas sobre a germinação e o desenvolvimento radicial de Lactuca sativa L. (alface, Handroanthus ochraceus (Cham. Mattos (ipê e Medicago sativa L. (alfafa. O extrato causou uma forte inibição na germinação das sementes de alface. O desenvolvimento radicial de alface e alfafa foi reduzido em 75 e 30%, respectivamente. Não foi observado efeito do extrato sobre a germinação ou o desenvolvimento radicial do ipê. Todas as espécies tiveram a velocidade de germinação reduzida. Observaram-se alterações micromorfológicas no ápice radicial das plântulas de alface.

  13. Bayesian Models for Astrophysical Data Using R, JAGS, Python, and Stan

    Science.gov (United States)

    Hilbe, Joseph M.; de Souza, Rafael S.; Ishida, Emille E. O.

    2017-05-01

    This comprehensive guide to Bayesian methods in astronomy enables hands-on work by supplying complete R, JAGS, Python, and Stan code, to use directly or to adapt. It begins by examining the normal model from both frequentist and Bayesian perspectives and then progresses to a full range of Bayesian generalized linear and mixed or hierarchical models, as well as additional types of models such as ABC and INLA. The book provides code that is largely unavailable elsewhere and includes details on interpreting and evaluating Bayesian models. Initial discussions offer models in synthetic form so that readers can easily adapt them to their own data; later the models are applied to real astronomical data. The consistent focus is on hands-on modeling, analysis of data, and interpretations that address scientific questions. A must-have for astronomers, its concrete approach will also be attractive to researchers in the sciences more generally.

  14. DEF- and GLO-like proteins may have lost most of their interaction partners during angiosperm evolution.

    Science.gov (United States)

    Melzer, Rainer; Härter, Andrea; Rümpler, Florian; Kim, Sangtae; Soltis, Pamela S; Soltis, Douglas E; Theißen, Günter

    2014-11-01

    DEFICIENS (DEF)- and GLOBOSA (GLO)-like proteins constitute two sister clades of floral homeotic transcription factors that were already present in the most recent common ancestor (MRCA) of extant angiosperms. Together they specify the identity of petals and stamens in flowering plants. In core eudicots, DEF- and GLO-like proteins are functional in the cell only as heterodimers with each other. There is evidence that this obligate heterodimerization contributed to the canalization of the flower structure of core eudicots during evolution. It remains unknown as to whether this strict heterodimerization is an ancient feature that can be traced back to the MRCA of extant flowering plants or if it evolved later during the evolution of the crown group angiosperms. The interactions of DEF- and GLO-like proteins of the early-diverging angiosperms Amborella trichopoda and Nuphar advena and of the magnoliid Liriodendron tulipifera were analysed by employing yeast two-hybrid analysis and electrophoretic mobility shift assay (EMSA). Character-state reconstruction, including data from other species as well, was used to infer the ancestral interaction patterns of DEF- and GLO-like proteins. The yeast two-hybrid and EMSA data suggest that DEF- and GLO-like proteins from early-diverging angiosperms both homo- and heterodimerize. Character-state reconstruction suggests that the ability to form heterodimeric complexes already existed in the MRCA of extant angiosperms and that this property remained highly conserved throughout angiosperm evolution. Homodimerization of DEF- and GLO-like proteins also existed in the MRCA of all extant angiosperms. DEF-like protein homodimerization was probably lost very early in angiosperm evolution and was not present in the MRCA of eudicots and monocots. GLO-like protein homodimerization might have been lost later during evolution, but very probably was not present in the MRCA of eudicots. The flexibility of DEF- and GLO-like protein interactions in

  15. SLAT/Def6 plays a critical role in the pathogenic process of experimental autoimmune uveitis (EAU).

    Science.gov (United States)

    Vistica, Barbara P; Shi, Guangpu; Nugent, Lindsey; Tan, Cuiyan; Altman, Amnon; Gery, Igal

    2012-01-01

    SWAP 70-like adaptor of T cells (SLAT; aka Def6) is a recently discovered guanine nucleotide exchange factor for Rho guanosine triphosphate (GTP)ases that has been previously shown to play a role in cluster of differentiation(CD)4+ T cell activation, T-helper (Th)1/Th2/Th17 differentiation and development of experimental autoimmune encephalomyelitis. Here, we investigated the role of SLAT/Def6 in the development of experimental autoimmune uveitis (EAU), an animal model for several uveitic conditions in humans. SLAT/Def6 deficient ("KO") mice and C57BL/6 controls were immunized with interphotoreceptor retinoid-binding protein (IRBP), along with pertussis toxin. The development of ocular inflammation was determined by both fundoscopy and histological examination. Lymphoid cells from draining lymph nodes were cultured with IRBP to measure lymphocyte proliferation and release of cytokines. Purified dendritic cells were tested for their capacity to present antigen to responding lymphocytes. In addition, the lymphoid cells were tested for the expression of forkhead box P3 (FoxP3), using conventional methods, and the activity of T-regulatory cells was determined by their capacity to inhibit in vitro proliferative responses. Serum anti -IRBP antibody levels were measured by enzyme-linked immunosorbant assay (ELISA). quantitative polymerase chain reaction (qPCR) was used to determine the transcript levels of cytokines in inflamed eyes. SLAT/Def6 KO mice had significantly reduced EAU compared to controls. Cells isolated from draining lymph nodes of SLAT/Def6 KO mice exhibited impaired proliferation and production of Th1 and Th17 signature cytokines (interferon [IFN]-γ and interleukin [IL]-17, respectively) when compared with cells isolated from control mice. qPCR of inflamed eyes detected similar levels of IFN-γ transcript in control and SLAT/Def6 KO mice, whereas the IL-17 transcript levels in eyes of the SLAT/Def6 KO mice were lower than in eyes of the controls. The SLAT/Def

  16. DEF- and GLO-like proteins may have lost most of their interaction partners during angiosperm evolution

    Science.gov (United States)

    Melzer, Rainer; Härter, Andrea; Rümpler, Florian; Kim, Sangtae; Soltis, Pamela S.; Soltis, Douglas E.; Theißen, Günter

    2014-01-01

    Background and Aims DEFICIENS (DEF)- and GLOBOSA (GLO)-like proteins constitute two sister clades of floral homeotic transcription factors that were already present in the most recent common ancestor (MRCA) of extant angiosperms. Together they specify the identity of petals and stamens in flowering plants. In core eudicots, DEF- and GLO-like proteins are functional in the cell only as heterodimers with each other. There is evidence that this obligate heterodimerization contributed to the canalization of the flower structure of core eudicots during evolution. It remains unknown as to whether this strict heterodimerization is an ancient feature that can be traced back to the MRCA of extant flowering plants or if it evolved later during the evolution of the crown group angiosperms. Methods The interactions of DEF- and GLO-like proteins of the early-diverging angiosperms Amborella trichopoda and Nuphar advena and of the magnoliid Liriodendron tulipifera were analysed by employing yeast two-hybrid analysis and electrophoretic mobility shift assay (EMSA). Character-state reconstruction, including data from other species as well, was used to infer the ancestral interaction patterns of DEF- and GLO-like proteins. Key Results The yeast two-hybrid and EMSA data suggest that DEF- and GLO-like proteins from early-diverging angiosperms both homo- and heterodimerize. Character-state reconstruction suggests that the ability to form heterodimeric complexes already existed in the MRCA of extant angiosperms and that this property remained highly conserved throughout angiosperm evolution. Homodimerization of DEF- and GLO-like proteins also existed in the MRCA of all extant angiosperms. DEF-like protein homodimerization was probably lost very early in angiosperm evolution and was not present in the MRCA of eudicots and monocots. GLO-like protein homodimerization might have been lost later during evolution, but very probably was not present in the MRCA of eudicots. Conclusions The

  17. Archaeological prospecting by DEF method; Denkai zansaho ni yoru iseki tansa

    Energy Technology Data Exchange (ETDEWEB)

    Kishikawa, H.; Aono, T.; Tanaka, T.; Mizunaga, H. [Kyushu University, Fukuoka (Japan). Faculty of Engineering

    1996-05-01

    A study is made of a newly-developed differential electric field (DEF) method, wherein potentials between potential electrodes equidistant from a current electrode is measured for directly detecting the secondary potential attributable to an anomalous-resistivity body. In this method, a current is fed into the ground from a point source C1, and four potential electrodes, two each on the X-axis and Y-axis, are provided equidistant from the point source C1 for the measurement of the potentials in the directions of X and Y. Numerical experiments and field experiments in a playground were conducted for this DEF method, and it was found that this method is effective in detecting an anomalous-resistivity bodies (ruins, etc.) situated in a homogeneous medium or in a horizontal multi-layer structure, is capable of displaying anomalous vectors enabling the estimation of the direction from the observation point of the anomalous-resistivity body, improves on work efficiency over the conventional mapping method, enables the estimation of the boundary of the anomalous-resistivity body on the basis of the peak of the anomalous electric field residue on the display, and that the obtained data can be easily processed by use of a personal computer on the site. 3 refs., 9 figs.

  18. Structural and functional studies of a phosphatidic acid-binding antifungal plant defensin MtDef4: Identification of an RGFRRR motif governing fungal cell entry

    Energy Technology Data Exchange (ETDEWEB)

    Sagaram, Uma S.; El-Mounadi, Kaoutar; Buchko, Garry W.; Berg, Howard R.; Kaur, Jagdeep; Pandurangi, Raghoottama; Smith, Thomas J.; Shah, Dilip

    2013-12-04

    A highly conserved plant defensin MtDef4 potently inhibits the growth of a filamentous fungus Fusarium graminearum. MtDef4 is internalized by cells of F. graminearum. To determine its mechanism of fungal cell entry and antifungal action, NMR solution structure of MtDef4 has been determined. The analysis of its structure has revealed a positively charged patch on the surface of the protein consisting of arginine residues in its γ-core signature, a major determinant of the antifungal activity of MtDef4. Here, we report functional analysis of the RGFRRR motif of the γ-core signature of MtDef4. The replacement of RGFRRR to AAAARR or to RGFRAA not only abolishes fungal cell entry but also results in loss of the antifungal activity of MtDef4. MtDef4 binds strongly to phosphatidic acid (PA), a precursor for the biosynthesis of membrane phospholipids and a signaling lipid known to recruit cytosolic proteins to membranes. Mutations of RGFRRR which abolish fungal cell entry of MtDef4 also impair its binding to PA. Our results suggest that RGFRRR motif is a translocation signal for entry of MtDef4 into fungal cells and that this positively charged motif likely mediates interaction of this defensin with PA as part of its antifungal action.

  19. Caracterización Fitoquímica y Efecto Hipoglucemiante de Tecoma stans y su Relación con la Presencia del Cromo como Factor de Tolerancia a la Glucosa Phytochemical Characterization and Hypoglycemic Effect of the Tecoma stans and its Relationship to the Presence of Chromium as a Factor for Glucose Tolerance

    Directory of Open Access Journals (Sweden)

    Ma. de J Ibarra

    2009-01-01

    Full Text Available Se realizó la caracterización fitoquímica y la determinación del cromo en hojas de Tecoma stans, observando su efecto hipoglucemiante en animales con diabetes mellitus inducida en forma experimental. Como hiperglucemiante se utilizó estreptozotocina en ratas machos Sprague-Dawley a una dosis de 45 mg/kg de peso. Los tratamientos experimentales, todos por vía oral, fueron los siguientes para grupos diabéticos y grupos no diabéticos: agua, infusión de la planta, picolinato de cromo, e infusión combinada con picolinato de cromo. Se encontró cromo en T. stans (54 µg/100g, la hiperglucemia disminuyó hasta 33.5 % con T. stans, 10.1% con picolinato de cromo y 32.3% con la combinación de T. stans y picolinato de cromo, en 45 días. Con este estudio se confirma el efecto hipoglucemiante de T. stans.Phytochemical characterization of Tecoma stans leaves, determination of chromium and their hypoglucemiant action in experimental animals with induced diabetes was done. The hyperglucemiant streptozotocin was employed in Sprague Dawley male rats at a dose of 45 mg/kg. The experimental treatments, all orally delivered, were the following for both diabetic and non diabetic groups: water, infusion of the plant, chromium picolinate and infusion combined with chromium picolinato. Chromium was found in T. stans (54 µg/100g, glucose levels decreased up to 33, 5 % with T. stans, 10, 1% with chromium picolinate and 32, 3% with the combination of T. stans and chromium picolinate, in 45 days. The hypoglucemiant effect of T. stans was confirmed with this study.

  20. Comparisons of rational engineering correlations of thermophoretically-augmented particle mass transfer with STAN5-predictions for developing boundary layers

    Science.gov (United States)

    Gokoglu, S. A.; Rosner, D. E.

    1984-01-01

    Modification of the code STAN5 to properly include thermophoretic mass transport, and examination of selected test cases developing boundary layers which include variable properties, viscous dissipation, transition to turbulence and transpiration cooling. Under conditions representative of current and projected GT operation, local application of St(M)/St(M),o correlations evidently provides accurate and economical engineering design predictions, especially for suspended particles characterized by Schmidt numbers outside of the heavy vapor range.

  1. Arubao为“挑战”DefCon和Black Hat安全大会

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Arubao无线网络公司近日宣布,在美国拉斯维加斯召开的“地下黑客”年度会议DefCon和安全专家年度会议Black Hat上,由其负责提供的无线基础架构成功击退了所有的入侵攻击企图。大会对网络日志的最终分析报告也证实了有数千起入侵企图均被成功检测并阻止。本次大会共有7500多名与会者出席了本年度的大会,

  2. The Cyber Defense (CyDef) Model for Assessing Countermeasure Capabilities.

    Energy Technology Data Exchange (ETDEWEB)

    Kimura, Margot [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); DeVries, Troy Robert [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Gordon, Susanna P. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-06-01

    Cybersecurity is essential to maintaining operations, and is now a de facto cost of business. Despite this, there is little consensus on how to systematically make decisions about cyber countermeasures investments. Identifying gaps and determining the expected return on investment (ROI) of adding a new cybersecurity countermeasure is frequently a hand-waving exercise at best. Worse, cybersecurity nomenclature is murky and frequently over-loaded, which further complicates issues by inhibiting clear communication. This paper presents a series of foundational models and nomenclature for discussing cybersecurity countermeasures, and then introduces the Cyber Defense (CyDef) model, which provides a systematic and intuitive way for decision-makers to effectively communicate with operations and device experts.

  3. DUFS and DEFS : development, reliability and validity of the Dutch Fatigue Scale and the Dutch Exertion Fatigue Scale

    NARCIS (Netherlands)

    Tiesinga, LJ; Dassen, TWN; Halfens, RJG

    1998-01-01

    The purpose of this study among patients with chronic heart failure (n = 138) was to develop and test both the Dutch Fatigue Scale (DUFS) and the Dutch Exertion Fatigue Scale (DEFS). Psychometric testing of these scales included measurement of internal consistency, construct validity and criterion-r

  4. simDEF: definition-based semantic similarity measure of gene ontology terms for functional similarity analysis of genes.

    Science.gov (United States)

    Pesaranghader, Ahmad; Matwin, Stan; Sokolova, Marina; Beiko, Robert G

    2016-05-01

    Measures of protein functional similarity are essential tools for function prediction, evaluation of protein-protein interactions (PPIs) and other applications. Several existing methods perform comparisons between proteins based on the semantic similarity of their GO terms; however, these measures are highly sensitive to modifications in the topological structure of GO, tend to be focused on specific analytical tasks and concentrate on the GO terms themselves rather than considering their textual definitions. We introduce simDEF, an efficient method for measuring semantic similarity of GO terms using their GO definitions, which is based on the Gloss Vector measure commonly used in natural language processing. The simDEF approach builds optimized definition vectors for all relevant GO terms, and expresses the similarity of a pair of proteins as the cosine of the angle between their definition vectors. Relative to existing similarity measures, when validated on a yeast reference database, simDEF improves correlation with sequence homology by up to 50%, shows a correlation improvement >4% with gene expression in the biological process hierarchy of GO and increases PPI predictability by > 2.5% in F1 score for molecular function hierarchy. Datasets, results and source code are available at http://kiwi.cs.dal.ca/Software/simDEF CONTACT: ahmad.pgh@dal.ca or beiko@cs.dal.ca Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  5. Priming effects on seed germination in Tecoma stans (Bignoniaceae) and Cordia megalantha (Boraginaceae), two tropical deciduous tree species

    Science.gov (United States)

    Alvarado-López, Sandra; Soriano, Diana; Velázquez, Noé; Orozco-Segovia, Alma; Gamboa-deBuen, Alicia

    2014-11-01

    Successful revegetation necessarily requires the establishment of a vegetation cover and one of the challenges for this is the scarce knowledge about germination and seedling establishment of wild tree species. Priming treatments (seed hydration during a specific time followed by seed dehydration) could be an alternative germination pre-treatment to improve plant establishment. Natural priming (via seed burial) promotes rapid and synchronous germination as well as the mobilisation of storage reserves; consequently, it increases seedling vigour. These metabolic and physiological responses are similar to those occurring as a result of the laboratory seed priming treatments (osmopriming and matrix priming) applied successfully to agricultural species. In order to know if natural priming had a positive effect on germination of tropical species we tested the effects of natural priming on imbibition kinetics, germination parameters (mean germination time, lag time and germination rate and percentage) and reserve mobilisation in the seeds of two tree species from a tropical deciduous forest in south-eastern México: Tecoma stans (L Juss. Ex Kunth) and Cordia megalantha (S.F Blake). The wood of both trees are useful for furniture and T. stans is a pioneer tree that promotes soil retention in disturbed areas. We also compared the effect of natural priming with that of laboratory matrix priming (both in soil). Matrix priming improved germination of both studied species. Natural priming promoted the mobilisation of proteins and increased the amount of free amino acids and of lipid degradation in T. stans but not in C. megalantha. Our results suggest that the application of priming via the burial of seeds is an easy and inexpensive technique that can improve seed germination and seedling establishment of tropical trees with potential use in reforestation and restoration practices.

  6. Development of 3D PPF/DEF scaffolds using micro-stereolithography and surface modification.

    Science.gov (United States)

    Lan, Phung Xuan; Lee, Jin Woo; Seol, Young-Joon; Cho, Dong-Woo

    2009-01-01

    Poly(propylene fumarate) (PPF) is an ultraviolet-curable and biodegradable polymer with potential applications for bone regeneration. In this study, we designed and fabricated three-dimensional (3D) porous scaffolds based on a PPF polymer network using micro-stereolithography (MSTL). The 3D scaffold was well fabricated with a highly interconnected porous structure and porosity of 65%. These results provide a new scaffold fabrication method for tissue engineering. Surface modification is a commonly used and effective method for improving the surface characteristics of biomaterials without altering their bulk properties that avoids the expense and long time associated with the development of new biomaterials. Therefore, we examined surface modification of 3D scaffolds by applying accelerated biomimetic apatite and arginine-glycine-aspartic acid (RGD) peptide coating to promote cell behavior. The apatite coating uniformly covered the scaffold surface after immersion for 24 h in 5-fold simulated body fluid (5SBF) and then the RGD peptide was applied. Finally, the coated 3D scaffolds were seeded with MC3T3-E1 pre-osteoblasts and their biologic properties were evaluated using an MTS assay and histologic staining. We found that 3D PPF/diethyl fumarate (DEF) scaffolds fabricated with MSTL and biomimetic apatite coating can be potentially used in bone tissue engineering.

  7. Investigations on some metabolites of Tecoma stans Juss. callus tissue. Part II. Chromatographical analysis of alkaloid and quinone compounds

    Directory of Open Access Journals (Sweden)

    Barbara Dohnal

    2015-01-01

    Full Text Available Tecoma stans Juss. callus tissue grown on Murashige-Mei Lie Lin (M-L and Murashige-Skoog (RT-k medium supplemented with Tecoma alkaloid precursors like lysine, mevalonic acid lactone and quinolinic acid, were investigated for their alkaloid content by thin-layer (TLC and paper (PC chromatography methods. The results were compared with those obtained by parallel analysis of greenhouse plant leaves. Seven alkaloid spots were detected in the leaf extracts, namely: actinidine, 4-noractinidine, boschniakine, tecomanine, two spots of skytanthine derivatives and one unidentified spot. Only l spot corresponding to skytanthine derivatives appeard in the extracts of callus tissues cultured on basal M-L medium or supplemented with lysine or mevalonic acid lactone. However, 3 alkaloid spots were revealed in extracts of homogeneous parenchyma-like callus tissues obtained o-n, RT-k medium. Beside the skytanthine derivatives rnentioned above, actinidine and tecomanine were identified, and moreover, in callus tissues growing on the same medium (RT-k supplemented with quinolinic acid, boschniakine was found. It was found that T. stans leaves as well as callus tissues cultured on RT-k medium contained traces of lapachol and another quinone-type compound.

  8. Presentation of the PyDEF post-treatment Python software to compute publishable charts for defect energy formation

    Science.gov (United States)

    Péan, Emmanuel; Vidal, Julien; Jobic, Stéphane; Latouche, Camille

    2017-03-01

    In this paper we present a new software, Python for Defect Energy Formation (PyDEF), especially dedicated to the calculation of defect formation energy including its various corrections as developed by the theoretical community through the years. This program offers an intuitive graphical user interface which allows one to easily compute the corrected formation energy of any defect using theoretical calculation results. PyDEF is particularly suited for semiconductor materials as it includes finite size error corrections due to spurious intercell interactions as it is able to plot the formation energy of the different charge states and to determine the more stable ones as a function of the Fermi energy. Moreover PyDEF is able to plot Density Of States (DOS) with various parameters such as the projection on atoms or atomic species allowing a more comprehensive representation of defect-related electronic states. This software is developed in such a way that even non specialist or coder can use it and easily and quickly obtain reliable results.

  9. Phosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells

    KAUST Repository

    Joshi, Rubin N.

    2017-09-25

    Regulatory T cells (Tregs) control key events of immune tolerance, primarily by suppression of effector T cells. We previously revealed that Tregs rapidly suppress T cell receptor (TCR)-induced calcium store depletion in conventional CD4CD25 T cells (Tcons) independently of IP levels, consequently inhibiting NFAT signaling and effector cytokine expression. Here, we study Treg suppression mechanisms through unbiased phosphoproteomics of primary human Tcons upon TCR stimulation and Treg-mediated suppression, respectively. Tregs induced a state of overall decreased phosphorylation as opposed to TCR stimulation. We discovered novel phosphosites (T595_S597) in the DEF6 (SLAT) protein that were phosphorylated upon TCR stimulation and conversely dephosphorylated upon coculture with Tregs. Mutation of these DEF6 phosphosites abrogated interaction of DEF6 with the IP receptor and affected NFAT activation and cytokine transcription in primary Tcons. This novel mechanism and phosphoproteomics data resource may aid in modifying sensitivity of Tcons to Treg-mediated suppression in autoimmune disease or cancer.

  10. STAN NIEUKOŃCZONEJ KONSTRUKCJI BUDYNKU OWT PO 25 LATACH OD REALIZACJI I PROPOZYCJA SPOSOBU ANALIZY STATYCZNEJ

    Directory of Open Access Journals (Sweden)

    Czesław MIEDZIAŁOWSKI

    2016-07-01

    Full Text Available Praca dotyczy stanu technicznego nieukończonej konstrukcji systemowego budynku OWT po 25 latach od realizacji. Przedstawiono stan wykonania tego obiektu oraz jakość robót, głównie w zakresie połączeń pod względem geometrii i odniesienia do wymagań katalogowych tego systemu. Pokazano stan połączeń ścian szczytowych ze ścianą podłużną oraz połączeń ścian zewnętrznych (tzw. belkościan ze ścianami poprzecznymi. Podano informacje co do stanu betonu i stali w konstrukcji tzn. w elementach i w złączach. Wskazano miejsca szczególnie zdegradowane i zagrożone korozją . Stwierdzono w trakcie analiz wbudowanie wadliwych prefabrykatów w zakresie geometrii i cech materiałowych oraz niewłaściwy montaż. Podano również efektywny sposób do analizy statycznej konstrukcji budynku w modelu trójwymiarowym, z uwzględnieniem podłoża gruntowego, wadliwego montażu i dodatkowych oddziaływań na konstrukcję. W podsumowaniu podano główne stwierdzone wady w omawianym budynku, które mogą stanowić zagrożenie bezpieczeństwa konstrukcji.

  11. Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) λ/ι.

    Science.gov (United States)

    Worthmann, Kirstin; Leitges, Michael; Teng, Beina; Sestu, Marcello; Tossidou, Irini; Samson, Thomas; Haller, Hermann; Huber, Tobias B; Schiffer, Mario

    2013-12-01

    The atypical protein kinase C (aPKC) isotypes PKCλ/ι and PKCζ are both expressed in podocytes; however, little is known about differences in their function. Previous studies in mice have demonstrated that podocyte-specific loss of PKCλ/ι leads to a severe glomerular phenotype, whereas mice deficient in PKCζ develop no renal phenotype. We analyzed various effects caused by PKCλ/ι and PKCζ deficiency in cultured murine podocytes. In contrast to PKCζ-deficient podocytes, PKCλ/ι-deficient podocytes exhibited a severe actin cytoskeletal phenotype, reduced cell size, decreased number of focal adhesions, and increased activation of small GTPases. Comparative microarray analysis revealed that the guanine nucleotide exchange factor Def-6 was specifically up-regulated in PKCλ/ι-deficient podocytes. In vivo Def-6 expression is significantly increased in podocytes of PKCλ/ι-deficient mice. Cultured PKCλ/ι-deficient podocytes exhibited an enhanced membrane association of Def-6, indicating enhanced activation. Overexpression of aPKCλ/ι in PKCλ/ι-deficient podocytes could reduce the membrane-associated expression of Def-6 and rescue the actin phenotype. In the present study, PKCλ/ι was identified as an important factor for actin cytoskeletal regulation in podocytes and Def-6 as a specific downstream target of PKCλ/ι that regulates the activity of small GTPases and subsequently the actin cytoskeleton of podocytes.

  12. 浅谈单相串激电机电枢元件的借偏%On the Def lection of Single-Phase Series Motor Armature Element

    Institute of Scientific and Technical Information of China (English)

    庄晓龙

    2014-01-01

    以电刷的放置原则为切入点,引出串激电机电枢元件的借偏概念,介绍电枢元件借偏大小判断的原理和基本步骤。%With the principle of placing the brush as a starting point, the concept of universal motor armature element def lection is pointed, and the principle and basic steps of armature elements def lection judgment is introduced.

  13. Potential of dispersion of Tecoma stans and chemical attributes of some soils of the Paraná state

    Directory of Open Access Journals (Sweden)

    Celina Wisniewski

    2009-03-01

    Full Text Available This work correlated invasiveness characteristic (potential dispersion of Tecoma stans (L. Jussieu ex. Kunth(BIGNONIACEAE known as a Yellow-Bell. Open field test was developed starting from stakes in vases with four different types soilsof the Paraná State, conduced to randomized block design with four treatments and five replications. The soils were analyzedregarding the pH, CTC, level of C, Al+³, macro and micronutrients, and content of sand, silt and clay. After 6 months the leaf area, dryweight of leaves and potential dispersion, calculated by given numeric values from 1 to 4 for phonological phases presented. Themacro and micronutrients content (except K and Fe were high in all the soils. The correlations between dispersion potential and pHand the V% were positive and significant and with effective CTC, the Fe and clay content were negative. It was not found significantcorrelations between the dispersion potential and biomass or leaf area. Positive and significant correlations of biomass and leaf areawith macro (except P and micronutrients (except Cu apparently indicate that if the evaluation had been accomplished at the end of theflowering period of the species, nutritional relationships with the dispersion potential would be clearer, although it can be concludedthat the species has a preference for less acid soils.

  14. The defensin from avocado (Persea americana var. drymifolia) PaDef induces apoptosis in the human breast cancer cell line MCF-7.

    Science.gov (United States)

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Salgado-Garciglia, Rafael; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

    2016-08-01

    Antimicrobial peptides (AMPs) are cytotoxic to cancer cells; however, mainly the effects of AMPs from animals have been evaluated. In this work, we assessed the cytotoxicity of PaDef defensin from avocado (Persea americana var. drymifolia) on the MCF-7 cancer cell line (a breast cancer cell line) and evaluated its mechanism of action. PaDef inhibited the viability of MCF-7 cells in a concentration-dependent manner, with an IC50=141.62μg/ml. The viability of normal peripheral blood mononuclear cells was unaffected by this AMP. Additionally, PaDef induced apoptosis in MCF-7 cells in a time-dependent manner, but did not affect the membrane potential or calcium flow. In addition, PaDef IC50 induced the expression of cytochrome c, Apaf-1, and the caspase 7 and 9 genes. Likewise, this defensin induced the loss of mitochondrial Δψm and increased the phosphorylation of MAPK p38, which may lead to MCF-7 apoptosis by the intrinsic pathway. This is the first report of an avocado defensin inducing intrinsic apoptosis in cancer cells, which suggests that it could be a potential therapeutic molecule in the treatment of cancer.

  15. NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions

    NARCIS (Netherlands)

    Portieles, R.; Ayra, C.; Gonzalez, E.; Gallo, A.; Rodriguez, R.; Chacón, O.; López, Y.; Rodriguez, M.; Castillo, J.; Pujol, M.; Enriquez, G.; Borroto, C.; Trujillo, L.; Thomma, B.P.H.J.; Borrás-Hidalgo, O.

    2010-01-01

    Plant defensins are small cysteine-rich peptides that inhibit the growth of a broad range of microbes. In this article, we describe NmDef02, a novel cDNA encoding a putative defensin isolated from Nicotiana megalosiphon upon inoculation with the tobacco blue mould pathogen Peronospora hyoscyami

  16. NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions

    NARCIS (Netherlands)

    Portieles, R.; Ayra, C.; Gonzalez, E.; Gallo, A.; Rodriguez, R.; Chacón, O.; López, Y.; Rodriguez, M.; Castillo, J.; Pujol, M.; Enriquez, G.; Borroto, C.; Trujillo, L.; Thomma, B.P.H.J.; Borrás-Hidalgo, O.

    2010-01-01

    Plant defensins are small cysteine-rich peptides that inhibit the growth of a broad range of microbes. In this article, we describe NmDef02, a novel cDNA encoding a putative defensin isolated from Nicotiana megalosiphon upon inoculation with the tobacco blue mould pathogen Peronospora hyoscyami f.sp

  17. Effects of nasal or pulmonary delivered treatments with an adenovirus vectored interferon (mDEF201 on respiratory and systemic infections in mice caused by cowpox and vaccinia viruses.

    Directory of Open Access Journals (Sweden)

    Donald F Smee

    Full Text Available An adenovirus 5 vector encoding for mouse interferon alpha, subtype 5 (mDEF201 was evaluated for efficacy against lethal cowpox (Brighton strain and vaccinia (WR strain virus respiratory and systemic infections in mice. Two routes of mDEF201 administration were used, nasal sinus (5-µl and pulmonary (50-µl, to compare differences in efficacy, since the preferred treatment of humans would be in a relatively small volume delivered intranasally. Lower respiratory infections (LRI, upper respiratory infections (URI, and systemic infections were induced by 50-µl intranasal, 10-µl intranasal, and 100-µl intraperitoneal virus challenges, respectively. mDEF201 treatments were given prophylactically either 24 h (short term or 56d (long-term prior to virus challenge. Single nasal sinus treatments of 10(6 and 10(7 PFU/mouse of mDEF201 protected all mice from vaccinia-induced LRI mortality (comparable to published studies with pulmonary delivered mDEF201. Systemic vaccinia infections responded significantly better to nasal sinus delivered mDEF201 than to pulmonary treatments. Cowpox LRI infections responded to 10(7 mDEF201 treatments, but a 10(6 dose was only weakly protective. Cowpox URI infections were equally treatable by nasal sinus and pulmonary delivered mDEF201 at 10(7 PFU/mouse. Dose-responsive prophylaxis with mDEF201, given one time only 56 d prior to initiating a vaccinia virus LRI infection, was 100% protective from 10(5 to 10(7 PFU/mouse. Improvements in lung hemorrhage score and lung weight were evident, as were decreases in liver, lung, and spleen virus titers. Thus, mDEF201 was able to treat different vaccinia and cowpox virus infections using both nasal sinus and pulmonary treatment regimens, supporting its development for humans.

  18. A Trypsin Inhibitor from Tecoma stans Leaves Inhibits Growth and Promotes ATP Depletion and Lipid Peroxidation in Candida albicans and Candida krusei

    Science.gov (United States)

    Patriota, Leydianne L. S.; Procópio, Thamara F.; de Souza, Maria F. D.; de Oliveira, Ana Patrícia S.; Carvalho, Lidiane V. N.; Pitta, Maira G. R.; Rego, Moacyr J. B. M.; Paiva, Patrícia M. G.; Pontual, Emmanuel V.; Napoleão, Thiago H.

    2016-01-01

    Tecoma stans (yellow elder) has shown medicinal properties and antimicrobial activity. Previous reports on antifungal activity of T. stans preparations and presence of trypsin inhibitor activity from T. stans leaves stimulated the investigation reported here. In this work, we proceeded to the purification and characterization of a trypsin inhibitor (TesTI), which was investigated for anti-Candida activity. Finally, in order to determine the potential of TesTI as a new natural chemotherapeutic product, its cytotoxicity to human peripheral blood mononuclear cells (PBMCs) was evaluated. TesTI was isolated from saline extract by ammonium sulfate fractionation followed by ion exchange and gel filtration chromatographies. Antifungal activity was evaluated by determining the minimal inhibitory (MIC) and fungicide (MFC) concentrations using fungal cultures containing only yeast form or both yeast and hyphal forms. Candida cells treated with TesTI were evaluated for intracellular ATP levels and lipid peroxidation. Cytotoxicity of TesTI to PBMCs was evaluated by MTT assay. TesTI (39.8 kDa, pI 3.41, Ki 43 nM) inhibited similarly the growth of both C. albicans and C. krusei culture types at MIC of 100 μg/mL. The MFCs were 200 μg/mL for C. albicans and C. krusei. Time-response curves revealed that TesTI (at MIC) was more effective at inhibiting the replication of C. albicans cells. At MIC, TesTI promoted reduction of ATP levels and lipid peroxidation in the Candida cells, being not cytotoxic to PBMCs. In conclusion, TesTI is an antifungal agent against C. albicans and C. krusei, without toxicity to human cells. PMID:27199940

  19. Fiskalne aspekty legalizacji miękkich narkotyków. Doświadczenia Stanów Zjednoczonych Ameryki Północnej

    OpenAIRE

    Tim, Artur

    2016-01-01

    W ostatnich latach zaobserwowano zmianę podejścia administracji federalnej Stanów Zjednoczonych Ameryki Północnej do zagadnienia zażywania marihuany. Nastąpiło przejście od wojny pochłaniającej wiele ofiar, do faktycznego pozostawienia decyzji o legalizacji marihuany poszczególnym stanom. Autor opisuje wskazany proces, przedstawia kontrowersje, jakie wywołał przełomowy wyrok w sprawie Gonzales v. Reich, analizuje status prawników z branży narkotykowej (tzw. „marijuana lawyers”)...

  20. Differential modulation of dibenzo[def,p]chrysene transplacental carcinogenesis: Maternal diets rich in indole-3-carbinol versus sulforaphane

    Energy Technology Data Exchange (ETDEWEB)

    Shorey, Lyndsey E.; Madeen, Erin P. [Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97331 (United States); Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331 (United States); Atwell, Lauren L.; Ho, Emily [Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331 (United States); School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR, 97331 (United States); Löhr, Christiane V. [Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331 (United States); College of Veterinary Medicine, Oregon State University, Corvallis, OR, 97331 (United States); Pereira, Clifford B. [Department of Statistics, Oregon State University, Corvallis, OR, 97331 (United States); Dashwood, Roderick H. [Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97331 (United States); Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331 (United States); Williams, David E., E-mail: david.williams@oregonstate.edu [Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97331 (United States); Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331 (United States)

    2013-07-01

    Cruciferous vegetable components have been documented to exhibit anticancer properties. Targets of action span multiple mechanisms deregulated during cancer progression, ranging from altered carcinogen metabolism to the restoration of epigenetic machinery. Furthermore, the developing fetus is highly susceptible to changes in nutritional status and to environmental toxicants. Thus, we have exploited a mouse model of transplacental carcinogenesis to assess the impact of maternal dietary supplementation on cancer risk in offspring. In this study, transplacental and lactational exposure to a maternal dose of 15 mg/Kg B.W. of dibenzo[def,p]chrysene (DBC) resulted in significant morbidity of offspring due to an aggressive T-cell lymphoblastic lymphoma. As in previous studies, indole-3-carbinol (I3C, feed to the dam at 100, 500 or 1000 ppm), derived from cruciferous vegetables, dose-dependently reduced lung tumor multiplicity and also increased offspring survival. Brussels sprout and broccoli sprout powders, selected for their relative abundance of I3C and the bioactive component sulforaphane (SFN), respectively, surprisingly enhanced DBC-induced morbidity and tumorigenesis when incorporated into the maternal diet at 10% wt/wt. Purified SFN, incorporated in the maternal diet at 400 ppm, also decreased the latency of DBC-dependent morbidity. Interestingly, I3C abrogated the effect of SFN when the two purified compounds were administered in equimolar combination (500 ppm I3C and 600 ppm SFN). SFN metabolites measured in the plasma of neonates positively correlated with exposure levels via the maternal diet but not with offspring mortality. These findings provide justification for further study of the safety and bioactivity of cruciferous vegetable phytochemicals at supplemental concentrations during the perinatal period. - Highlights: • Dietary supplementation may modulate cancer risk in a mouse model of lymphoma. • Cruciferous vegetables may not contain sufficient I3C

  1. High Def Resolution

    CERN Multimedia

    2008-01-01

    According to the report, the Times Square Ball in New York scheduled to drop later that night had spent much of 2007 being renovated and remodeled so that it could now proudly display over 16 million colors. At the time, I didn't think much of it, but later on, my thoughts turned not to sex, like so many of my peers (and certain fruit flies I could mention), but back to this magic colorful ball.

  2. Gateway技术构建转基因重组质粒pRP.EX3d-EF1A-LRP16-His-IRES-eGFP%Application of gateway technology in construction of recombinant transgenic vector pRP. EX3d-EF1A-LRP16-His-IRES-eGFP

    Institute of Scientific and Technical Information of China (English)

    柏苗苗; 王春萌; 伍志强; 梅倩; 李小雷; 李祥; 赵亚力; 韩为东

    2014-01-01

    Objective To lay the foundation for establishing transgenic mice by constructing recombinant transgenic vector pRP. EX3d-EF1A-LRP16-His-IRES-eGFP. Methods The attB1- LRP16-His-attB2 was amplified by overlap PCR. The LRP16 gene was cloned into pDown vector via BP reaction and transmitted into pRP.EX3d vector via LR reaction with pDown-LRP16-His, pUp-EF1A, pTail-IRES-eGFP and PRP. Des3d. The recombinant plasmid was transformed into Stbl3 cells and screened using the ampicillin (AMP) resistance gene. Positive clones were confirmed by PCR and DNA sequencing, respectively. Results Sequencing and restriction endonuclease showed that the recombinant vector pRP.EX3d-EF1A-LRP16-His-IRES-eGFP was successfully constructed. Conclusion The successfully constructed recombinant vector pRP.EX3d-EF1A-LRP16-His-IRES-eGFP can be used in establishing LRP16 transgenic mice.%目的:构建转基因重组质粒pRP.EX3d-EF1A-LRP16-His-IRES-eGFP,为转基因鼠的建立奠定基础。方法利用重叠PCR技术扩增attB1-LRP16-His-attB2,经BP反应,将LRP16基因插入到载体pDown上,pUp-EF1A、pDown-LRP16-His、pTail-IRES-eGFP和PRP.Des3d经LR反应,将LRP16转移到pRP.EX3d上,转化Stbl3细胞,用氨苄西林进行抗性筛选,经PCR鉴定后将阳性克隆送测序。结果测序及酶切结果验证获得正确的pRP.EX3d-EF1A-LRP16-His-IRES-eGFP重组质粒。结论成功构建pRP.EX3d-EF1A-LRP16-His-IRES-eGFP重组质粒,可用于下一步LRP16转基因小鼠的构建。

  3. Human in Vivo Pharmacokinetics of [(14)C]Dibenzo[def,p]chrysene by Accelerator Mass Spectrometry Following Oral Microdosing.

    Science.gov (United States)

    Madeen, Erin; Corley, Richard A; Crowell, Susan; Turteltaub, Kenneth; Ognibene, Ted; Malfatti, Mike; McQuistan, Tammie J; Garrard, Mary; Sudakin, Dan; Williams, David E

    2015-01-20

    Dibenzo(def,p)chrysene (DBC), (also known as dibenzo[a,l]pyrene), is a high molecular weight polycyclic aromatic hydrocarbon (PAH) found in the environment, including food, produced by the incomplete combustion of hydrocarbons. DBC, classified by IARC as a 2A probable human carcinogen, has a relative potency factor (RPF) in animal cancer models 30-fold higher than benzo[a]pyrene. No data are available describing the disposition of high molecular weight (>4 rings) PAHs in humans to compare to animal studies. Pharmacokinetics of DBC was determined in 3 female and 6 male human volunteers following oral microdosing (29 ng, 5 nCi) of [(14)C]-DBC. This study was made possible with highly sensitive accelerator mass spectrometry (AMS), capable of detecting [(14)C]-DBC equivalents in plasma and urine following a dose considered of de minimus risk to human health. Plasma and urine were collected over 72 h. The plasma Cmax was 68.8 ± 44.3 fg·mL(-1) with a Tmax of 2.25 ± 1.04 h. Elimination occurred in two distinct phases: a rapid (α)-phase, with a T1/2 of 5.8 ± 3.4 h and an apparent elimination rate constant (Kel) of 0.17 ± 0.12 fg·h(-1), followed by a slower (β)-phase, with a T1/2 of 41.3 ± 29.8 h and an apparent Kel of 0.03 ± 0.02 fg·h(-1). In spite of the high degree of hydrophobicity (log Kow of 7.4), DBC was eliminated rapidly in humans, as are most PAHs in animals, compared to other hydrophobic persistent organic pollutants such as, DDT, PCBs and TCDD. Preliminary examination utilizing a new UHPLC-AMS interface, suggests the presence of polar metabolites in plasma as early as 45 min following dosing. This is the first in vivo data set describing pharmacokinetics in humans of a high molecular weight PAH and should be a valuable addition to risk assessment paradigms.

  4. The historical significance of the Trepça mine in the Region of Stan-Terg during the twentieth century

    Directory of Open Access Journals (Sweden)

    Mustafë Haziri

    2015-11-01

    Full Text Available The historical significance of the Trepça’s mine in Stan-Terg is so big, that it is impossible to be presented in this scientific paper, because of its historic, economic and social character. This paper analyses chronologically the importance of Trepça mine, focusing with particular emphasis on the period of the twentieth century where in 1926 the first research that was implemented by the British government began. In 1930 began the modern exploitation of Trepça which was followed by some union movements which lasted until 1939. In 1941, Trepça fell into the hands of the nazi regime of Germany. Other aspects of this work include the period of the Second World War, when the nationalization of property was implemented during the communist regime in former Yugoslavia until the great strike of 1989.

  5. Efecto del estrés osmótico sobre la germinación de semillas de Tecoma stans (Bignoniaceae)

    OpenAIRE

    2016-01-01

    La., germinación de semillas de Tecoma stans (Bignoniaceae) se determinó' en' cuatro concentraciones de polietilenglicol durante un mes. La germinación fue fuertemente innibición a -1.0 MPa (8.6 %) y fue totalmente bloqueada a -1.5 MPa. La germinación en los tratamientos a -0.5, -0.1 Y testigo fueron 72, 88 Y 88.6 %, respectivamente. El índice de germinación (lG) indicó una reducción de la tasa de germinación conforme el potencial osmótico de la solución decreció. No se observaron daños en aq...

  6. Investigations on some metabolites of Tecoma stans Juss. callus tissue. Part III. Chromatographical search for iridoids, phenolic acids, terpenoids and sugars

    Directory of Open Access Journals (Sweden)

    Barbara Dohnal

    2015-01-01

    Full Text Available Tissus cultures of Tecoma stans Juss. cultivated on modified Murashige-Skoog medium (RT-k were phytochemically analysed by means of chromatographical methods (PC, TLC. The following products were found as metabolites: phenolic acids - chlorogenics, caffeic, ferulic, vanillic, o-coumaric and sinapic; steroids - β-sitosterol; triterpenes - ursolic and oleanolic acids, α-amyrine; sugars - glucose, fructose, sucrose, xylose. Meso-inositol was isolated in 0.8% yield. In intact plant leaves, some differences concerning the content and/or number of individual compounds were observed, namely: lack of sinapic acid and occurrence of p-coumaric acid, lower content of β-sitosterol, lack of oleanolic acid, occurrence of β-amyrine and of one unidentified triterpenoid, lack of xylose, occurrence of maltose, raffinose, and stachiose. The level of mesoinositol inn leaves was distincly lower than in the callus tissues. Neither in callus tissues nor in leaves iridoid glycosides were found.

  7. Bi-sulphotellurides associated with Pb - Bi - (Sb ± Ag, Cu, Fe) sulphosalts: an example from the Stan Terg deposit in Kosovo

    Science.gov (United States)

    Kołodziejczyk, Joanna; Pršek, Jaroslav; Voudouris, Panagiotis Ch.; Melfos, Vasilios

    2017-08-01

    New mineralogical and mineral-chemical data from the Stan Terg deposit, Kosovo, revealed the presence of abundant Bi-sulphotellurides associated with Bi- and Sb-sulphosalts and galena in pyrite-pyrrhotite-rich skarn-free ore bodies (ores without skarn minerals). The Bi-bearing association comprises Bi-sulphotellurides (joséite-A, joséite-B, unnamed phase A with a chemical formula close to (Bi,Pb)2(TeS)2, unnamed phase B with a chemical composition close to (Bi,Pb)2.5Te1.5S1.5), ikunolite, cosalite, Sb-lillianite, members of the kobellite series and Bi-jamesonite. Compositional trends of the Bi-sulphotellurides suggest lattice-scale incorporation of Bi-(Pb)-rich module and/or admixture with submicroscopic PbS layers in modulated structures, or complicated Bi-Te substitution. Cosalite is characterized by high Sb (max. 3.94 apfu), and low Cu and Ag (up to 0.72 apfu of Cu+Ag). Jamesonite from this mineralization has elevated Bi content, from 0.85 to 2.30 apfu. The negligible content of Au and Ag in the Bi-sulphotellurides, the low content of Ag in Bi-sulphosalts, together with the lack of Au-Ag bearing phases in the mineralization, indicate either ore deposition from fluid(s) depleted in precious metals, or physico-chemical conditions of ore formation preventing Au and Ag precipitation at the deposit site. The temperature of initial mineralization may have exceeded 400 °C as suggested by the lamellar exsolution textures observed in lillianite, which indicate breakdown textures from decomposition of high-temperature initial crystals. Non-stoichiometric phases among the Bi-sulphosalts and sulphotellurides studied at Stan Terg reflect modulated growth processes in a metasomatic environment.

  8. The binding of triclosan to SmeT, the repressor of the multidrug efflux pump SmeDEF, induces antibiotic resistance in Stenotrophomonas maltophilia.

    Directory of Open Access Journals (Sweden)

    Alvaro Hernández

    2011-06-01

    Full Text Available The wide utilization of biocides poses a concern on the impact of these compounds on natural bacterial populations. Furthermore, it has been demonstrated that biocides can select, at least in laboratory experiments, antibiotic resistant bacteria. This situation has raised concerns, not just on scientists and clinicians, but also on regulatory agencies, which are demanding studies on the impact that the utilization of biocides may have on the development on resistance and consequently on the treatment of infectious diseases and on human health. In the present article, we explored the possibility that the widely used biocide triclosan might induce antibiotic resistance using as a model the opportunistic pathogen Stenotrophomonas maltophilia. Biochemical, functional and structural studies were performed, focusing on SmeDEF, the most relevant antibiotic- and triclosan-removing multidrug efflux pump of S. maltophilia. Expression of smeDEF is regulated by the repressor SmeT. Triclosan released SmeT from its operator and induces the expression of smeDEF, thus reducing the susceptibility of S. maltophilia to antibiotics in the presence of the biocide. The structure of SmeT bound to triclosan is described. Two molecules of triclosan were found to bind to one subunit of the SmeT homodimer. The binding of the biocide stabilizes the N terminal domain of both subunits in a conformation unable to bind DNA. To our knowledge this is the first crystal structure obtained for a transcriptional regulator bound to triclosan. This work provides the molecular basis for understanding the mechanisms allowing the induction of phenotypic resistance to antibiotics by triclosan.

  9. The binding of triclosan to SmeT, the repressor of the multidrug efflux pump SmeDEF, induces antibiotic resistance in Stenotrophomonas maltophilia.

    Directory of Open Access Journals (Sweden)

    Alvaro Hernández

    2011-06-01

    Full Text Available The wide utilization of biocides poses a concern on the impact of these compounds on natural bacterial populations. Furthermore, it has been demonstrated that biocides can select, at least in laboratory experiments, antibiotic resistant bacteria. This situation has raised concerns, not just on scientists and clinicians, but also on regulatory agencies, which are demanding studies on the impact that the utilization of biocides may have on the development on resistance and consequently on the treatment of infectious diseases and on human health. In the present article, we explored the possibility that the widely used biocide triclosan might induce antibiotic resistance using as a model the opportunistic pathogen Stenotrophomonas maltophilia. Biochemical, functional and structural studies were performed, focusing on SmeDEF, the most relevant antibiotic- and triclosan-removing multidrug efflux pump of S. maltophilia. Expression of smeDEF is regulated by the repressor SmeT. Triclosan released SmeT from its operator and induces the expression of smeDEF, thus reducing the susceptibility of S. maltophilia to antibiotics in the presence of the biocide. The structure of SmeT bound to triclosan is described. Two molecules of triclosan were found to bind to one subunit of the SmeT homodimer. The binding of the biocide stabilizes the N terminal domain of both subunits in a conformation unable to bind DNA. To our knowledge this is the first crystal structure obtained for a transcriptional regulator bound to triclosan. This work provides the molecular basis for understanding the mechanisms allowing the induction of phenotypic resistance to antibiotics by triclosan.

  10. Rivew and Update of Methods in Codex Stan 234-1999 and a Guide to Work on Physical and Chemical Standards in China%“CODEX STAN 234-1999的回审和更新”对中国理化检验方法标准工作的启示和借鉴

    Institute of Scientific and Technical Information of China (English)

    王紫菲; 肖晶

    2016-01-01

    对法典方法标准“分析和采样的推荐方法”(CODEX STAN 234-1999)简要介绍,着重分析国际食品法典分析和采样方法委员会对其开展的回审、更新等工作,为中国食品安全国家标准体系中理化检验方法标准工作的开展提供了工作思路和可借鉴的经验。%An overview of the review and update process of codex stan 234-1999 has been presented,by which some valuable guidelines and experience could be given to further developments of work on national physical and chemical standards in china. Additionally,the discussons and conclusions refer to this subject in the CCMAS meetings have also been shown in this paper.

  11. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Jaquelina Julia Guzmán-Rodríguez

    2013-01-01

    Full Text Available Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP represent attractive control agents. Mexican avocado (Persea americana var. drymifolia is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp encoding a protein (78 aa homologous with plant defensins (>80%. We expressed the defensin PaDef cDNA (pBME3 in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%. Also, S. aureus viability was inhibited from 50 μg/mL total protein (27–38% but was more evident at 100 μg/mL (52–65%. This inhibition was higher than the effect showed by polyclonal population (~23%. Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

  12. Antibacterial activity of defensin PaDef from avocado fruit (Persea americana var. drymifolia) expressed in endothelial cells against Escherichia coli and Staphylococcus aureus.

    Science.gov (United States)

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Suárez-Rodríguez, Luis M; Salgado-Garciglia, Rafael; Rodríguez-Zapata, Luis C; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E

    2013-01-01

    Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 μg/mL total protein (27-38%) but was more evident at 100 μg/mL (52-65%). This inhibition was higher than the effect showed by polyclonal population (~23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

  13. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus

    Science.gov (United States)

    Guzmán-Rodríguez, Jaquelina Julia; López-Gómez, Rodolfo; Suárez-Rodríguez, Luis M.; Salgado-Garciglia, Rafael; Rodríguez-Zapata, Luis C.; Ochoa-Zarzosa, Alejandra; López-Meza, Joel E.

    2013-01-01

    Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 μg/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 μg/mL total protein (27–38%) but was more evident at 100 μg/mL (52–65%). This inhibition was higher than the effect showed by polyclonal population (~23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens. PMID:24319695

  14. Effective empirical corrections for basis set superposition error in the def2-SVPD basis: gCP and DFT-C

    Science.gov (United States)

    Witte, Jonathon; Neaton, Jeffrey B.; Head-Gordon, Martin

    2017-06-01

    With the aim of mitigating the basis set error in density functional theory (DFT) calculations employing local basis sets, we herein develop two empirical corrections for basis set superposition error (BSSE) in the def2-SVPD basis, a basis which—when stripped of BSSE—is capable of providing near-complete-basis DFT results for non-covalent interactions. Specifically, we adapt the existing pairwise geometrical counterpoise (gCP) approach to the def2-SVPD basis, and we develop a beyond-pairwise approach, DFT-C, which we parameterize across a small set of intermolecular interactions. Both gCP and DFT-C are evaluated against the traditional Boys-Bernardi counterpoise correction across a set of 3402 non-covalent binding energies and isomerization energies. We find that the DFT-C method represents a significant improvement over gCP, particularly for non-covalently-interacting molecular clusters. Moreover, DFT-C is transferable among density functionals and can be combined with existing functionals—such as B97M-V—to recover large-basis results at a fraction of the cost.

  15. Tolerancia experimental de las especies vegetales Nicotiana glauca, Jacaranda mimosifolia, Tecoma stans, Medicago sativa y Spinacea oleracea al boro, en Argentina

    Directory of Open Access Journals (Sweden)

    Marta L. de Viana

    2008-09-01

    Full Text Available La actividad de las industrias borateras constituye una fuente puntual y difusa de contaminación del aire, suelo y aguas superficiales y profundas. Por lo tanto, el estudio y experimentación acerca de las posibles formas de contrarrestar este impacto constituye una prioridad. Una técnica relativamente nueva para descontaminar suelos es la fitorremediación, que emplea plantas y microorganismos asociados. El primer paso es detectar las especies vegetales tolerantes, lo que constituye el objetivo de este trabajo. Se realizó un experimento en laboratorio para evaluar la germinación, la supervivencia y el crecimiento de distintas especies en diferentes concentraciones de boro. Al comienzo y al final del experimento se determinó la concentración de boro en el sustrato para cada tratamiento y para sustratos con y sin vegetación. Se encontraron diferencias significativas debidas al tratamiento, la especie y la interacción especie *tratamiento. M. sativa, N. glauca y J. mimosifolia fueron las especies de mayor tolerancia al boro. Las otras especies presentaron una disminución en todas las variables-respuesta en función de la concentración del contaminante. Todas presentaron una baja supervivencia en la máxima concentración. La disminución de boro fue máxima en el tratamiento de 30 ppm de boro con M. sativa y la menor se registró en los tratamiento de 20 ppm de boro con J. mimosifolia y de 30 ppm de boro con T. stans y S. oleraceae. Se concluye que N. glauca, M. sativa y J. mimosifolia podrían considerarse como prometedoras en remediación.Experimental tolerance to boron of the plant species Nicotiana glauca, Jacaranda mimosifolia, Tecoma stans, Medicago sativa y Spinacea oleracea in Argentina. The activity of boron industries is a punctual and diffuse source of air, soil and water pollution. Therefore, it is a priority to study possible ways of reducing this impact. A relatively new technology for reducing soil pollution is

  16. 解读国际标准CODEX STAN 206《乳品术语通则》——兼论本土奶牛养殖业的竞争力优势

    Institute of Scientific and Technical Information of China (English)

    顾佳升; 李梅

    2013-01-01

    本文以国际食品法典CODEX STAN 206标准为主,以其他国际标准为辅,与我国相关标准作比较分析,进而从法规和标准的角度,探索中国本土奶牛养殖业的竞争力优势所在及其生存底线.

  17. Analysis of Tecoma stans infestation in the rural area of Bandeirantes county, Paraná State, Brazil/ Análise da infestação do amarelinho (Tecoma stans na zona rural do município de Bandeirantes – PR

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Reis

    2008-08-01

    Full Text Available The anthropic exploration of native areas and the introduction of exotic species resulted in the process of biological contamination, responsible for environmental and economical damages. Biological invasions are considered the second worst threat to biodiversity. This work had the objective of identifying the infestation index of the invader exotic species Tecoma stans. It is aggressive and hard to control and spreads easily by winged seeds and vegetative structures. The work was carried out in the county of Bandeirantes – PR, and the T. stans infestations patches were identified by GPS after field visits. The informations were processed in the Georeferenced Information Processing System - SPRING 4.1. There were identified 72 patches, predominantly in the pastures areas, river adjacent forests and forest fragments borders. Among the 16 microbasins of the county, infestation patches were identified in 10. There were 20, 17, 11, 7 and 7 patches with higher incidence, in Água do Cateto, Água da Cabiúna, Ribeirão dos Índios, Água das Perobas and Água das Antas, respectively. The analysis of spatial distribution revealed that the patches present the tendency to group up to 2.000 m away. The actual situation found in the analyzed areas is concerning, due to the high levels of T. stans infestations. The species is present in many of the neglect areas and puts in risk the forest remainders close to these areas.A exploração antrópica de áreas nativas e a introdução de espécies exóticas resultaram no processo de contaminação biológica, responsável por danos ambientais e econômicos. Invasões biológicas são consideradas a segunda maior ameaça a biodiversidade. Este estudo objetivou identificar o índice de infestação da planta exótica invasora, Tecoma stans, o amarelinho. Agressiva e de difícil controle, dissemina-se por sementes aladas e via vegetativa. O trabalho foi realizado no município de Bandeirantes – PR, os pontos

  18. In vitro metabolism of benzo[a]pyrene-7,8-dihydrodiol and dibenzo[def,p]chrysene-11,12 diol in rodent and human hepatic microsomes

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Jordan N.; Mehinagic, Denis; Nag, Subhasree; Crowell, Susan R.; Corley, Richard A.

    2017-03-01

    Polycyclic aromatic hydrocarbons (PAHs) are contaminants that are ubiquitously found in the environment, produced through combustion of organic matter or petrochemicals, and many of which are procarcinogens. The prototypic PAH, benzo[a]pyrene (B[a]P) and the highly carcinogenic dibenzo[def,p]chrysene (DBC) are metabolically activated by isoforms of the P450 enzyme superfamily producing benzo[a]pyrene-7,8-dihydrodiol (B[a]P diol), dibenzo[def,p]chrysene-11,12 diol (DBC diol). Each of these diols can be further metabolized by cytochrome P450 enzymes to highly reactive diol-epoxide metabolites that readily react with DNA or by phase II conjugation facilitating excretion. To complement prior in vitro metabolism studies with parent B[a]P and DBC, both phase I metabolism and phase II glucuronidation of B[a]P diol and DBC diol were measured in hepatic microsomes from female B6129SF1/J mice, male Sprague-Dawley rats, and female humans. Metabolic parameters, including intrinsic clearance and Michaelis-Menten kinetics were calculated from substrate depletion data. Mice and rats demonstrated similar B[a]P diol phase I metabolic rates. Compared to rodents, human phase I metabolism of B[a]P diol demonstrated lower overall metabolic capacity, lower intrinsic clearance at higher substrate concentrations (>0.14 µM), and higher intrinsic clearance at lower substrate concentrations (<0.07 µM). Rates of DBC diol metabolism did not saturate in mice or humans and were highest overall in mice. Higher affinity constants and lower capacities were observed for DBC diol glucuronidation compared to B[a]P diol glucuronidation; however, intrinsic clearance values for these compounds were consistent within each species. Kinetic parameters reported here will be used to extend physiologically based pharmacokinetic (PBPK) models to include the disposition of B[a]P and DBC metabolites in animal models and humans to support future human health risk assessments.

  19. In vitro metabolism of benzo[a]pyrene-7,8-dihydrodiol and dibenzo[def,p]chrysene-11,12 diol in rodent and human hepatic microsomes.

    Science.gov (United States)

    Smith, Jordan N; Mehinagic, Denis; Nag, Subhasree; Crowell, Susan R; Corley, Richard A

    2017-01-21

    Polycyclic aromatic hydrocarbons (PAHs) are contaminants that are ubiquitously found in the environment, produced through combustion of organic matter or petrochemicals, and many of which are procarcinogens. The prototypic PAH, benzo[a]pyrene (B[a]P) and the highly carcinogenic dibenzo[def,p]chrysene (DBC) are metabolically activated by isoforms of the P450 enzyme superfamily producing benzo[a]pyrene-7,8-dihydrodiol (B[a]P diol), dibenzo[def,p]chrysene-11,12 diol (DBC diol). Each of these diols can be further metabolized by cytochrome P450 enzymes to highly reactive diol-epoxide metabolites that readily react with DNA or by phase II conjugation facilitating excretion. To complement prior in vitro metabolism studies with parent B[a]P and DBC, both phase I metabolism and phase II glucuronidation of B[a]P diol and DBC diol were measured in hepatic microsomes from female B6129SF1/J mice, male Sprague-Dawley rats, and female humans. Metabolic parameters, including intrinsic clearance and Michaelis-Menten kinetics were calculated from substrate depletion data. Mice and rats demonstrated similar B[a]P diol phase I metabolic rates. Compared to rodents, human phase I metabolism of B[a]P diol demonstrated lower overall metabolic capacity, lower intrinsic clearance at higher substrate concentrations (>0.14μM), and higher intrinsic clearance at lower substrate concentrations (P diol glucuronidation; however, intrinsic clearance values for these compounds were consistent within each species. Kinetic parameters reported here will be used to extend physiologically based pharmacokinetic (PBPK) models to include the disposition of B[a]P and DBC metabolites in animal models and humans to support future human health risk assessments.

  20. Polycyclic aromatic hydrocarbons as skin carcinogens: Comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse

    Energy Technology Data Exchange (ETDEWEB)

    Siddens, Lisbeth K.; Larkin, Andrew [Department of Environmental and Molecular Toxicology, Oregon State University (United States); Superfund Research Center, Oregon State University (United States); Krueger, Sharon K. [Superfund Research Center, Oregon State University (United States); The Linus Pauling Institute, Oregon State University (United States); Bradfield, Christopher A. [McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53706 (United States); Waters, Katrina M.; Tilton, Susan C. [Superfund Research Center, Oregon State University (United States); Computational Biology and Bioinformatics Group, Pacific Northwest National Laboratory, Richland, WA 99352 (United States); Pereira, Cliff B. [Superfund Research Center, Oregon State University (United States); Deptartment of Statistics, Oregon State University, Corvallis, OR 97331 (United States); Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331 (United States); Löhr, Christiane V. [Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331 (United States); College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331 (United States); Arlt, Volker M.; Phillips, David H. [Analytical and Environmental Sciences Division, MRC-HPA Centre for Environment and Health, King' s College London, London SE1 9NH (United Kingdom); Williams, David E., E-mail: david.williams@oregonstate.edu [Department of Environmental and Molecular Toxicology, Oregon State University (United States); Superfund Research Center, Oregon State University (United States); The Linus Pauling Institute, Oregon State University (United States); Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331 (United States); and others

    2012-11-01

    The polycyclic aromatic hydrocarbon (PAH), benzo[a]pyrene (BaP), was compared to dibenzo[def,p]chrysene (DBC) and combinations of three environmental PAH mixtures (coal tar, diesel particulate and cigarette smoke condensate) using a two stage, FVB/N mouse skin tumor model. DBC (4 nmol) was most potent, reaching 100% tumor incidence with a shorter latency to tumor formation, less than 20 weeks of 12-O-tetradecanoylphorbol-13-acetate (TPA) promotion compared to all other treatments. Multiplicity was 4 times greater than BaP (400 nmol). Both PAHs produced primarily papillomas followed by squamous cell carcinoma and carcinoma in situ. Diesel particulate extract (1 mg SRM 1650b; mix 1) did not differ from toluene controls and failed to elicit a carcinogenic response. Addition of coal tar extract (1 mg SRM 1597a; mix 2) produced a response similar to BaP. Further addition of 2 mg of cigarette smoke condensate (mix 3) did not alter the response with mix 2. PAH-DNA adducts measured in epidermis 12 h post initiation and analyzed by {sup 32}P post‐labeling, did not correlate with tumor incidence. PAH‐dependent alteration in transcriptome of skin 12 h post initiation was assessed by microarray. Principal component analysis (sum of all treatments) of the 922 significantly altered genes (p < 0.05), showed DBC and BaP to cluster distinct from PAH mixtures and each other. BaP and mixtures up-regulated phase 1 and phase 2 metabolizing enzymes while DBC did not. The carcinogenicity with DBC and two of the mixtures was much greater than would be predicted based on published Relative Potency Factors (RPFs). -- Highlights: ► Dibenzo[def,p]chrysene (DBC), 3 PAH mixtures, benzo[a]pyrene (BaP) were compared. ► DBC and 2 PAH mixtures were more potent than Relative Potency Factor estimates. ► Transcriptome profiles 12 hours post initiation were analyzed by microarray. ► Principle components analysis of alterations revealed treatment-based clustering. ► DBC gave a unique

  1. 抗生素诱导嗜麦芽窄食单胞菌临床株外排泵 SmeDEF 表达的研究%The Study of Antibiotic-Induced Expression of SmeDEF Efflux Pump in Stenotrophomonas Maltophilia

    Institute of Scientific and Technical Information of China (English)

    岳玮; 谭薇; 李晓明

    2013-01-01

    Objective To investigate the changes of antibiotic resistance in two clinical isolates of Smaltophilia and the repres -sion level of efflux pump SmeDEF induced by antibiotics .Methods The resistance of clinical strains was compared and the resistance chan -ges were investigated of clinical strains incubation in the LB broth ,containing different sub-inhibitory concentration(1 /2,1 /4 MIC) levofloxa-cin.The mRNA level of smeD was investigated by RTP -PCR.Results The two antibiotic-sensitive strains performed positive or negative to pump inhibits.The levofloxacin MICof of two strains after induced by the levofloxacin were higher by 1 ~3 dilution than before,but the tuime-thoprim-sulfamethoxazole MIChad showed no significant change .After using the efflux pump inhibitor CCCP ,MIC of two induced strains sig-nificantly reduced,but one could not be restored to the level that before induced .The level of mRNA of smeD of both antibiotic -induced strains rose.Conclusion The antibiotic resistance of Stenotrophomonas maltophilia clinical isolates induced by levofloxacin had increased , which was related to the expression of efflux pump Sme DEF .%  目的了解嗜麦芽窄食单胞菌2株临床分离株在抗生素诱导下耐药性和外排泵 SmeDEF 表达的变化。方法比较左氧氟沙星诱导前后嗜麦芽窄食单胞菌的 MIC 值,在不同亚抑菌浓度(1/2,1/4MIC)左氧氟沙星中培育后的菌株 MIC 变化。监测左氧氟沙星诱导前后菌株外排泵 SmeD 基因 RT-PCR 扩增后的表达水平。结果对抗生素敏感的两株菌泵抑制呈阳性或者阴性表现。两株菌经左氧氟沙星诱导后对左氧氟沙星的MIC 值比诱导前高1~3个稀释度,复方新诺明的 MIC 无明显变化。加用泵抑制 CCCP 后,2株诱导后的菌株MIC 值有较明显的降低,但其中1株不能恢复到诱导前水平。泵抑制剂阳性菌株抗生素过夜组和对数生长期添加组均明显高于无抗生素组的 SmeD 的表达。结论

  2. Satellite Tracking Astrometric Network (STAN)

    Science.gov (United States)

    Vecchiato, Alberto; Gai, Mario

    2015-08-01

    The possibility of precise orbit tracking and determination of different types of satellites has been explored for at least some 25 years (Arimoto et al., 1990). Proposals in this sense made use mainly of astrometric observations, but multiple tracking techniques combining transfer and laser ranging was also suggested (Guo et al., 2009; Montojo et al., 2011), with different requirements and performances ranging from $\\sim100$~m to tenths of meters.In this work we explore the possible improvements and a novel implementation of a technique relying on large angle, high precision astrometry from ground for the determination of satellite orbits. The concept is based on combined observation of geostationary satellites and other near-Earth space objects from two or more telescopes, applying the triangulation principle over widely separated regions of the sky. An accuracy of a few $10^{-2}$~m can be attained with 1-meter-class telescopes and a field of vied of some arcminutes.We discuss the feasibility of the technique, some of the implementation aspects, and the limitations imposed by atmospheric turbulence. The potential benefits for satellite orbit control and navigation systems are presented, depending on the number and position of the contributing telescopes.We also discuss the possibility that, by reversing the roles of stars and satellites, the same kind of observations can be used for verification and maintenance of astrometric catalogs.

  3. Gateway技术构建转基因重组质粒pRP.EX3d-EF1A-LRP16-His-IRES-eGFP

    Institute of Scientific and Technical Information of China (English)

    柏苗苗; 王春萌; 伍志强; 梅倩; 李小雷; 李祥; 赵亚力; 韩为东

    2014-01-01

    目的:构建转基因重组质粒pRP.EX3d-EF1A-LRP16-His-IRES-eGFP,为转基因鼠的建立奠定基础。方法利用重叠PCR技术扩增attB1-LRP16-His-attB2,经BP反应,将LRP16基因插入到载体pDown上,pUp-EF1A、pDown-LRP16-His、pTail-IRES-eGFP和PRP.Des3d经LR反应,将LRP16转移到pRP.EX3d上,转化Stbl3细胞,用氨苄西林进行抗性筛选,经PCR鉴定后将阳性克隆送测序。结果测序及酶切结果验证获得正确的pRP.EX3d-EF1A-LRP16-His-IRES-eGFP重组质粒。结论成功构建pRP.EX3d-EF1A-LRP16-His-IRES-eGFP重组质粒,可用于下一步LRP16转基因小鼠的构建。

  4. Polycyclic aromatic hydrocarbons as skin carcinogens: comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse.

    Science.gov (United States)

    Siddens, Lisbeth K; Larkin, Andrew; Krueger, Sharon K; Bradfield, Christopher A; Waters, Katrina M; Tilton, Susan C; Pereira, Cliff B; Löhr, Christiane V; Arlt, Volker M; Phillips, David H; Williams, David E; Baird, William M

    2012-11-01

    The polycyclic aromatic hydrocarbon (PAH), benzo[a]pyrene (BaP), was compared to dibenzo[def,p]chrysene (DBC) and combinations of three environmental PAH mixtures (coal tar, diesel particulate and cigarette smoke condensate) using a two stage, FVB/N mouse skin tumor model. DBC (4nmol) was most potent, reaching 100% tumor incidence with a shorter latency to tumor formation, less than 20 weeks of 12-O-tetradecanoylphorbol-13-acetate (TPA) promotion compared to all other treatments. Multiplicity was 4 times greater than BaP (400 nmol). Both PAHs produced primarily papillomas followed by squamous cell carcinoma and carcinoma in situ. Diesel particulate extract (1 mg SRM 1650b; mix 1) did not differ from toluene controls and failed to elicit a carcinogenic response. Addition of coal tar extract (1 mg SRM 1597a; mix 2) produced a response similar to BaP. Further addition of 2 mg of cigarette smoke condensate (mix 3) did not alter the response with mix 2. PAH-DNA adducts measured in epidermis 12 h post initiation and analyzed by ³²P post-labeling, did not correlate with tumor incidence. PAH-dependent alteration in transcriptome of skin 12 h post initiation was assessed by microarray. Principal component analysis (sum of all treatments) of the 922 significantly altered genes (p<0.05), showed DBC and BaP to cluster distinct from PAH mixtures and each other. BaP and mixtures up-regulated phase 1 and phase 2 metabolizing enzymes while DBC did not. The carcinogenicity with DBC and two of the mixtures was much greater than would be predicted based on published Relative Potency Factors (RPFs).

  5. Mutational and gene expression analysis of mtrDEF, omcA and mtrCAB during arsenate and iron reduction in Shewanella sp. ANA-3.

    Science.gov (United States)

    Reyes, Carolina; Murphy, Julie N; Saltikov, Chad W

    2010-07-01

    Arsenate respiration and Fe(III) reduction are important processes that influence the fate and transport of arsenic in the environment. The goal of this study was to investigate the impact of arsenate on Fe(III) reduction using arsenate and Fe(III) reduction deficient mutants of Shewanella sp. strain ANA-3. Ferrihydrite reduction in the absence of arsenate was similar for an arsenate reduction mutant (arrA and arsC deletion strain of ANA-3) compared with wild-type ANA-3. However, the presence of arsenate adsorbed onto ferrihydrite impeded Fe(III) reduction for the arsenate reduction mutant but not in the wild-type. In an Fe(III) reduction mutant (mtrDEF, omcA, mtrCAB null mutant of ANA-3), arsenate was reduced similarly to wild-type ANA-3 indicating the Fe(III) reduction pathway is not required for ferrihydrite-associated arsenate reduction. Expression analysis of the mtr/omc gene cluster of ANA-3 showed that omcA and mtrCAB were expressed under soluble Fe(III), ferrihydrite and arsenate growth conditions and not in aerobically grown cells. Expression of arrA was greater with ferrihydrite pre-adsorbed with arsenate relative to ferrihydrite only. Lastly, arrA and mtrA were simultaneously induced in cells shifted to anaerobic conditions and exposed to soluble Fe(III) and arsenate. These observations suggest that, unlike Fe(III), arsenate can co-induce operons (arr and mtr) implicated in arsenic mobilization.

  6. Transcriptional Profiling of Dibenzo[def,p]chrysene-induced Spleen Atrophy Provides Mechanistic Insights into its Immunotoxicity in MutaMouse.

    Science.gov (United States)

    Chepelev, Nikolai L; Long, Alexandra S; Williams, Andrew; Kuo, Byron; Gagné, Rémi; Kennedy, Dean A; Phillips, David H; Arlt, Volker M; White, Paul A; Yauk, Carole L

    2016-01-01

    Dibenzo[def,p]chrysene (DBC) is the most carcinogenic polycyclic aromatic hydrocarbon (PAH) examined to date. We investigated the immunotoxicity of DBC, manifested as spleen atrophy, following acute exposure of adult MutaMouse males by oral gavage. Mice were exposed to 0, 2.0, 6.2, or 20.0 mg DBC /kg-bw per day, for 3 days. Genotoxic endpoints (DBC-DNA adducts and lacZ mutant frequency in spleen and bone marrow, and red blood cell micronucleus frequency) and global gene expression changes were measured. All of the genotoxicity measures increased in a dose-dependent manner in spleen and bone marrow. Gene expression analysis showed that DBC activates p53 signaling pathways related to cellular growth and proliferation, which was evident even at the low dose. Strikingly, the expression profiles of DBC exposed mouse spleens were highly inversely correlated with the expression profiles of the only published toxicogenomics dataset of enlarged mouse spleen. This analysis suggested a central role for Bnip3l, a pro-apoptotic protein involved in negative regulation of erythroid maturation. RT-PCR confirmed expression changes in several genes related to apoptosis, iron metabolism, and aryl hydrocarbon receptor signaling that are regulated in the opposite direction during spleen atrophy versus benzo[a]pyrene-mediated splenomegaly. In addition, benchmark dose modeling of toxicogenomics data yielded toxicity estimates that are very close to traditional toxicity endpoints. This work illustrates the power of toxicogenomics to reveal rich mechanistic information for immunotoxic compounds and its ability to provide information that is quantitatively similar to that derived from standard toxicity methods in health risk assessment.

  7. Human Microdosing with Carcinogenic Polycyclic Aromatic Hydrocarbons: In Vivo Pharmacokinetics of Dibenzo[def,p]chrysene and Metabolites by UPLC Accelerator Mass Spectrometry.

    Science.gov (United States)

    Madeen, Erin P; Ognibene, Ted J; Corley, Richard A; McQuistan, Tammie J; Henderson, Marilyn C; Baird, William M; Bench, Graham; Turteltaub, Ken W; Williams, David E

    2016-10-17

    Metabolism is a key health risk factor following exposures to pro-carcinogenic polycyclic aromatic hydrocarbons (PAHs) such as dibenzo[def,p]chrysene (DBC), an IARC classified 2A probable human carcinogen. Human exposure to PAHs occurs primarily from the diet in nonsmokers. However, little data is available on the metabolism and pharmacokinetics in humans of high molecular weight PAHs (≥4 aromatic rings), including DBC. We previously determined the pharmacokinetics of DBC in human volunteers orally administered a microdose (29 ng; 5 nCi) of [(14)C]-DBC by accelerator mass spectrometry (AMS) analysis of total [(14)C] in plasma and urine. In the current study, we utilized a novel "moving wire" interface between ultraperformance liquid chromatography (UPLC) and AMS to detect and quantify parent DBC and its major metabolites. The major [(14)C] product identified in plasma was unmetabolized [(14)C]-DBC itself (Cmax = 18.5 ±15.9 fg/mL, Tmax= 2.1 ± 1.0 h), whereas the major metabolite was identified as [(14)C]-(+/-)-DBC-11,12-diol (Cmax= 2.5 ±1.3 fg/mL, Tmax= 1.8 h). Several minor species of [(14)C]-DBC metabolites were also detected for which no reference standards were available. Free and conjugated metabolites were detected in urine with [(14)C]-(+/-)-DBC-11,12,13,14-tetraol isomers identified as the major metabolites, 56.3% of which were conjugated (Cmax= 35.8 ± 23.0 pg/pool, Tmax = 6-12 h pool). [(14)C]-DBC-11,12-diol, of which 97.5% was conjugated, was also identified in urine (Cmax = 29.4 ± 11.6 pg/pool, Tmax = 6-12 h pool). Parent [(14)C]-DBC was not detected in urine. This is the first data set to assess metabolite profiles and associated pharmacokinetics of a carcinogenic PAH in human volunteers at an environmentally relevant dose, providing the data necessary for translation of high dose animal models to humans for translation of environmental health risk assessment.

  8. Estudio y caracterización del Autoinyectable de atropina-oxima DEF: diseño de nuevos Autoinyectables para Defensa NBQ y otros medicamentos de urgencia

    Directory of Open Access Journals (Sweden)

    J.J. Sánchez Ramos

    2014-03-01

    Full Text Available Antecedentes: Los autoinyectables (AI son dispositivos para administración de medicamentos de urgencia por vía parenteral (i.m. por el afectado o un compañero. Tienen interés militar en Defensa NBQ para la administración de antídotos contra agentes nerviosos y otros medicamentos de urgencia. Se estiman necesarios cinco nuevos AI que pueden ser fabricados por los Servicios Farmacéuticos de la Defensa, además del que se fabrica en la actualidad. Objetivos: Presentación del Autoinyectable AJP (AI-AJP y su preparado Autoinyectable de atropina-oxima DEF (AI-AOD, resultantes de la I+D sobre AI en el CEMILFARDEF, y su caracterización. Aplicación de resultados al diseño de cinco nuevos AI de interés en Defensa NBQ. Métodos: Ante la falta de ensayos y criterios de valoración oficiales para AI, se realiza una revisión bibliográfica y un estudio retrospectivo sobre la investigación básica llevada a cabo durante dos décadas en el desarrollo y fabricación del AI-AJP, estableciendo procedimientos de caracterización y evaluación de la calidad farmacéutica y propiedades funcionales en condiciones normales y de estrés. Mediante programas ACAD y ensayos galénicos se diseñan cinco nuevos AI. Resultados: Obtención del AI-AJP y su presentación AI-AOD. Presentación de sus componentes, funcionamiento y resultados del análisis de las variables implicadas en su diseño, producción y control: usabilidad, portabilidad, funcionamiento, fuerzas, inyectabilidad, contenidos, esterilidad, partículas, pirógenos, estabilidad, y resistencia. Se expone el diseño de cinco nuevos AI, a los que son aplicables los resultados obtenidos en este estudio. Conclusión: El Autoinyectable AJP cumple los requisitos exigidos por el estado actual de la ciencia, es seguro en funcionamiento, y robusto en resistencia al estrés estático y dinámico. Es estable y la inyección consistente. Se han diseñado cinco nuevos AI (Atropina, Diazepam, Multicomponentes

  9. Kirit C. Shah, M.D. v. Stan Harris and Nancy Harris. "Construction of Legal Arguments, Statutes of Limitations, and Medical Malpractice." Lesson Plans for Secondary Teachers on How Lawyers Prepare Their Arguments. Courts in the Classroom: Curriculum Concepts and Other Information on Indiana's Courts for the K-12 Educator.

    Science.gov (United States)

    Osborn, Elizabeth

    Stan and Nancy Harris filed a complaint against Kirit C. Shah, M.D., for misdiagnosing Mr. Harris's illness, charging Dr. Shah with negligence and asking for damages. A medical malpractice action in Indiana is governed by a two year statute of limitations. Because the Harrises failed to bring their action against Dr. Shah within this two year…

  10. Gheorghe Racoviţă, Ana-Maria Stan, Memoria Documentelor. Cooperarea franco-română la universitatea din Cluj, oglindită în arhiva lui Emil Racoviţă

    OpenAIRE

    2010-01-01

    L’ouvrage de Gheorghe Racoviţă et d’Ana-Maria Stan est un travail d’édition critique rédigé en roumain mais qui présente une grande partie des textes analysés dans leur langue d’origine, à savoir le français, ce qui en fait un outil précieux pour le chercheur ne maîtrisant pas la langue roumaine. Ce livre s’appuie sur les archives privées de la famille Racoviţă qui a conservé une partie de la correspondance d’Emil Racoviţă, le grand zoologue roumain, père de la bio-spéléologie et titulaire de...

  11. Characterization of Final Action Official Method(SM) 2011.19 and First Action Official Method 2015.06 Performance at Analyte Levels Corresponding to CODEX STAN 72 (1981) Minimum Levels.

    Science.gov (United States)

    Thompson, Joseph J; Pacquette, Lawrence H

    2017-03-01

    A limited single-laboratory validation (SLV) was conducted in the authors' laboratory to investigate the performance of AOAC Official MethodsSM 2011.19 Determination of Chromium (Cr), Selenium (Se), and Molybdenum (Mo) in Infant Formula and Adult Nutritional Products by Inductively Coupled Plasma/Mass Spectrometry and 2015.06 Determination of Minerals and Trace Elements in Infant Formula and Adult/Pediatric Nutritional Formula by Inductively Coupled Plasma/Mass Spectrometry at analyte levels below the practical LOQs (PLOQs) already published for these Final Action Official Methods. This work was needed to verify that the actual LOQs were below the minimum requirements for minerals in infant formula as given in CODEX STAN 72 (1981). Linearity studies at low levels were conducted as well as the analysis of blanks over multiple days to establish the LOQs (as opposed to PLOQs) for these nutrients. Several placebo matrixes from the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) program were tested over multiple days at two different sample sizes to quantitate the effect of doubling the sample size given in the original publications. The SLV results indicate that both methods can meet the Codex minimum requirements as-is, without modification of the methods, albeit with a relaxation of the stringent precision criteria originally established for these methods by SPIFAN. Precision can be improved by doubling the sample size, but this step is not necessary to use the method for its intended purpose. A concurrent collaborative study of Method 2015.06 showed that the RSDR obtained across eight laboratories for several infant formula placebos containing mineral concentrations between the PLOQ and LOQ were indeed worse than SPIFAN expectations, but reasonable Horwitz ratios (HorRat) were nonetheless obtained for these analytes.

  12. In vitro activity of levofloxacin against planktonic and biofilm Stenotrophomonas maltophilia lifestyles under conditions relevant to pulmonary infection in cystic fibrosis, and relationship with SmeDEF multidrug efflux pump expression.

    Science.gov (United States)

    Pompilio, Arianna; Crocetta, Valentina; Verginelli, Fabio; Bonaventura, Giovanni Di

    2016-07-01

    The activity of levofloxacin against planktonic and biofilm Stenotrophomonas maltophilia cells and the role played by the multidrug efflux pump SmeDEF were evaluated under conditions relevant to the cystic fibrosis (CF) lung. MIC, MBC and MBEC of levofloxacin were assessed, against five CF strains, under 'standard' (CLSI-recommended) and 'CF-like' (pH 6.8, 5% CO2, in a synthetic CF sputum) conditions. Levofloxacin was tested against biofilms at concentrations (10, 50 and 100 μg mL(-1)) corresponding to achievable serum levels and sputum levels by aerosolisation. smeD expression was evaluated, under both conditions, in planktonic and biofilm cells by RT-PCR. The bactericidal effect of levofloxacin was decreased, in three out of five strains tested, under 'CF-like' conditions (MBC: 2-4 vs 8-16 μg mL(-1), under 'standard' and 'CF-like' conditions, respectively). Biofilm was intrinsically resistant to levofloxacin, regardless of conditions tested (MBECs ≥ 100 μg mL(-1) for all strains). Only under 'CF-like' conditions, smeD expression increased during planktonic-to-biofilm transition, and in biofilm cells compared to stationary planktonic cells. Our findings confirmed that S. maltophilia biofilm is intrinsically resistant to therapeutic concentrations of levofloxacin. Under conditions relevant to CF, smeD overexpression could contribute to levofloxacin resistance. Further studies are warranted to define the clinical relevance of our findings.

  13. Psychologia prokreacji - stan obecny i perspektywy

    OpenAIRE

    Bielawska-Batorowicz, Eleonora

    2002-01-01

    The paper describes a new psychological discipline – reproductive psychology that deals with psychological processes and behaviour of people involved in procreation. However reproductive psychology is regarded as separate discipline it is closely connected to clinical, social, developmental and health psychology as well as psychology of personality. Main methodological issues – research paradigms and research tools were also discussed. The last part gives short presentation of new...

  14. Internet Banking in Paki stan: Finding Complexities

    Directory of Open Access Journals (Sweden)

    Mohammed Ather Akhlaq

    2011-04-01

    Full Text Available Technology is evolving every new day and has its impact on every thing. In this paper we will analyze the acceptance of technology in the Banking Industry of Pakis t a n. This res earch investigates complexities (factors that have resisted or obstructed the adoption of Internet Banking in Pakistan. Traditional innovation diffusion model (IDT is used to develop the research model indicating the proposed complexities and acceptance of Internet Banking among Pakistani users. T he model is tested with a survey sample (n=45. Out of the nine complexities identi fied, six are found significant. The findings of the research signify that 67 % of the proposed complexities, Familiarity with the technology, Concerns for Download Speed, Unfriendly Website Design, Fear of Government tracking transactions, Security and People Gaining Access/Misusing, hinder the users in accepting and adopting internet banking technology in Pakistan

  15. Human Microdosing with Carcinogenic Polycyclic Aromatic Hydrocarbons: In Vivo Pharmacokinetics of Dibenzo[ def,p ]chrysene and Metabolites by UPLC Accelerator Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Madeen, Erin P.; Ognibene, Ted J.; Corley, Richard A.; McQuistan, Tammie J.; Henderson, Marilyn C.; Baird, William M.; Bench, Graham; Turteltaub, Ken W.; Williams, David E.

    2016-10-17

    Metabolism is a key health risk factor following exposures to pro-carcinogenic polycyclic aromatic hydrocarbons (PAHs) such as dibenzo[def,p]chrysene (DBC), an IARC classified 2A probable human carcinogen. Human exposure to PAHs occurs primarily from the diet in non-smokers. However, little data is available on the metabolism and pharmacokinetics in humans of high molecular weight PAHs (≥4 aromatic rings), including DBC. We previously determined the pharmacokinetics of DBC in human volunteers orally administered a micro-dose (29 ng; 5 nCi) of [14C]-DBC by accelerator mass spectrometry (AMS) analysis of total [14C] in plasma and urine. In the current study, we utilized a novel “moving wire” interface between ultra-performance liquid chromatography (UPLC) and AMS to detect and quantify parent DBC and its major metabolites. The major [14C] product identified in plasma was unmetabolized [14C]-DBC itself, (Cmax= 18.5 ± 15.9 fg/mL, Tmax= 2.1 ± 1.0 h), whereas the major metabolite was identified as [14C]-(+/-)-DBC-11,12-diol (Cmax= 2.5 ± 1.3 fg/mL, Tmax= 1.8 h). Several minor species of [14C]-DBC metabolites were also detected for which no reference standards were available. Free and conjugated metabolites were detected in urine with [14C]-(+/-)-DBC-11,12,13,14-tetraol isomers identified as the major metabolites, 56.3% of which were conjugated (Cmax= 35.8 ± 23.0 pg/pool, Tmax= 6-12 h pool). [14C]-DBC-11,12-diol, of which 97.5% was conjugated, was also identified in urine (Cmax= 29.4 ± 11.6 pg/pool, Tmax= 6-12 h pool). Parent [14C]-DBC was not detected in urine. This is the first dataset to assess metabolite profiles and associated pharmacokinetics of a carcinogenic PAH in human volunteers at an environmentally relevant dose, providing the data necessary for translation of high dose animal models to humans for translation of environmental health risk assessment.

  16. Team Fuzing. Mitigation of FMU-139 Component Obsolescence

    Science.gov (United States)

    2007-11-02

    solvents, fire-fighting agents & fresh/saltwater. (Def Stan 00-35) To determine the ability of equipment to withstand prolonged storage at extreme (hot...switching regulator – Higher efficiency toroidal inductor – All 3 select resistors eliminated • Components are predominantly surface mount • Reverse

  17. Optimism kasvab, aga head ajad ei tule tagasi? / Stan Nahkor

    Index Scriptorium Estoniae

    Nahkor, Stan

    2011-01-01

    Heaoluühiskondadesse jõuab üha selgemini arusaamine, et eluiga pikeneb ja rahvastik vananeb, levib ka ebakindlus riiklike pensionide jätkusuutlikkuse suhtes. Seda ilmestab hoiuste mahu järjekindel kasv. Pankade probleemiks on peale vähenevate tulude ka suurenev valitsussektori sekkumine

  18. Kindlustussektorit häirib riigi sekkumine / Stan Nahkor

    Index Scriptorium Estoniae

    Nahkor, Stan

    2011-01-01

    Neli tippjuhti viiest peab riigipoolset ülereguleerimist kindlustussektori kriitiliseks probleemiks. PwC ja CSFI uuring näitab, et kindlustussektori jaoks on seaduste ülemäärane regulatsioon kahe aastaga suurenenud olulisimaks riskiallikaks

  19. Optimism kasvab, aga head ajad ei tule tagasi? / Stan Nahkor

    Index Scriptorium Estoniae

    Nahkor, Stan

    2011-01-01

    Heaoluühiskondadesse jõuab üha selgemini arusaamine, et eluiga pikeneb ja rahvastik vananeb, levib ka ebakindlus riiklike pensionide jätkusuutlikkuse suhtes. Seda ilmestab hoiuste mahu järjekindel kasv. Pankade probleemiks on peale vähenevate tulude ka suurenev valitsussektori sekkumine

  20. Kindlustussektorit häirib riigi sekkumine / Stan Nahkor

    Index Scriptorium Estoniae

    Nahkor, Stan

    2011-01-01

    Neli tippjuhti viiest peab riigipoolset ülereguleerimist kindlustussektori kriitiliseks probleemiks. PwC ja CSFI uuring näitab, et kindlustussektori jaoks on seaduste ülemäärane regulatsioon kahe aastaga suurenenud olulisimaks riskiallikaks

  1. Prokaryotic Expression of DEV Extracellular gD Gene and Its Subcellular Localization in Virus-infected DEF%鸭肠炎病毒gD基因胞外区的原核表达及在感染鸭胚成纤维细胞中的定位

    Institute of Scientific and Technical Information of China (English)

    刘琰; 徐凝; 付培芬; 母晓宇; 董井泉; 马波; 王君伟

    2012-01-01

    The extracellular gD gene was amplified by PCR from DEV genomic DNA according to our laboratory discovered gene sequence (GenBank accession number EU714267). The recombi-nant expression plasmid pET-30a-gDw could effectively express gDw fusion protein that was used to immunize rabbits. The titer of antibody tested by agar diffusion reaction was 1:16. Plaque reduction neutralization test showed that its neutralization antibody titer was up to 1:64. By indi-rected immunofluorescencei the results showed that gD appeared in the cell cytoplasm near the nuclear as early as 4 hours post infection. After 12 hours post infection, the fluorescence in cytoplasm was increased. Above all, the results afforded significant data for the study on the function of DEV gD.%以本实验室获得的鸭肠炎病毒(DEV) C-KCE株US区基因序列(GenBank登录号EU714267)为基础PCR扩增出gD基因胞外区(gDw),将其定向克隆至原核表达载体pET-30a(+)并转化至大肠杆菌RosettaTM(DE3)pLys.经IPTG诱导表达,SDS-PAGE分析表明gD基因胞外区在大肠杆菌中获得与预期大小相符的表达蛋白.重组蛋白纯化后免疫家兔制备多克隆抗体,血清琼扩效价达1∶16,噬斑减数中和试验测定血清中和抗体效价达1∶64.通过免疫荧光技术首次对gD进行亚细胞定位检测,结果表明DEV感染鸭胚成纤维细胞(DEF)后4h近核区域细胞质内首先开始出现荧光,12h以后显著增加,明显的点状绿色荧光广泛存在于胞质中.本研究为进一步开展DEV gD的功能研究提供了重要数据和材料.

  2. Stan liderom dlja samogo sebja / Mihkel Pärjamäe

    Index Scriptorium Estoniae

    Pärjamäe, Mihkel

    2007-01-01

    Liidriks olemise eri vormidest tänapäeval. Inimeste jagunemisest liidriteks ja mitteliidriteks. Mitteliidritest kui probleemist ühiskonnas. Domineerimisest kui omadusest, mis ei kuulu liidriomaduste hulka. Liidriomaduste arendamisest. Esimene samm liidriks olemise poole: hakata liidriks iseenda jaoks, st. kujundada visioon omaenda tulevikust, töötada välja strateegia ja hakata seda järjekindlalt ellu viima

  3. Immune mechanisms and immuno-therapy in sepsis: where do we stan d?

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Sepsis and septic shock have continued to produce significant morbidity and mortality, in recent times, in acutely injured patients as well as patients in the intensive care units despite advances in antibiotic therapy and in the cardi ovascular and pulmonary support for these patients. This emphasizes the need to gain a better understanding of the fundamental mechanisms of the pathogenesis of sepsis syndrome in the critically ill or injured patients. The present knowled ge of the mechanisms of septic pathogenesis clearly indicates involvement of mod ulations in the functions of the cells of body's immune defense system, namely, monocytes/macrophages, polymorphonuclear leukocytes, and lymphocytes. Most of su ch knowledge has been derived from laboratory experiments in animal models of se ptic injury, or from studies of immune-system cells, in vitro. Clinical stud ies have also supported the role of immune perturbations. Yet, to date, very few the rapeutic approaches are available to effectively counter the sepsis-related immu ne disturbances. Functional modulations in the immune system cells, in the injured/septic hos ts, can exert not only adaptive/beneficial effects but also profoundly adverse effects on the non-immune-system cells such as endothelial, epithelial, neurona l, endocrine, neuro-endocrine, smooth muscle, skeletal muscle, and cardiac muscl e cells. The primary functional modulation in the immune-system cells after inju ry or with critical illness is activation of such cells via molecules from pat hogens, for example, lipopolysaccharide from gram negative organisms, lipoteic hoic acid/peptidoglycan from gram positive organisms, or zymosan from fungi. Suc h pathogenic molecules activate monocytes and tissue macrophages to result in th e expression and release of cytokine mediators (TNFα, IL-1, IL-6, IL-8, and IL- 10), as well as certain lipid mediators (PGE2, LTB4, PAF). While these media tors could play a host-defense role in support of the host via containment/destr uctio n of the pathogens, they could also exert detrimental effects in the host and co ntribute to host morbidity and mortality. Some of these mediators (TNFα, IL-1, IL-6, IL-8, LTB4, PAF) have been shown to be “pro-inflammatory”,and to potenti al ly exert a harmful effect on non-immune cell systems such as endothelial cells, epithelial cells, and muscle cells. Among the pro-inflammatory mediators, TNFα a nd IL-1 could play major harmful roles, and thus contribute to the injured host morbidity and mortality. Mediators, IL-10 and PGE2 have been shown to be “an ti-inflammatory” and to potentially contribute to a dreadful state of immuno-s uppression in the injured hosts, which can also lead to morbidity and mortality. Whi ch is worse, a harmful pro-inflammatory phase or harmful immuno-suppression ? Or Which occurs first, a harmful pro-inflammatory condition or a harmful immuno-su ppression? These are questions which can not be definitively answered for the g eneral population of critically ill/injured patients. It is reasonable to assum e that the answers to these question would vary from one patient subset to anot her patient subset. Thus, whether to treat the patient with a putative anti-pro- inflammatory agent or with a putative anti-anti-inflammatory agent remai ns unresolved for the general sepsis patient population. Paradoxically, TNFα, IL-1, and other pro-inflammatory mediators under certa in circumstances may serve as natural “blockers” of immuno-suppression or “pr omoters” of immune stimulation. This may be true in the case of some of the se ptic patients. Understandably, these patients should not be treated with anti-pro-inf lammatory agents. On the other hand, the anti-inflammatory mediators such as PGE 2, IL-10, and certain other naturally occurring antagonists of TNFα and IL-10 a ctions (TNFα, and IL-1 receptor antagonists) could not only be producing a cert ai n level of immune-suppression but also serving as important feed back controller of the pro-inflammatory mediators. Thus some of the naturally occurring anti-I nflammatory agents could indeed serve as adaptive/beneficial “anti-pro-inflamma tory”, therapeutic agents in certain subset of sepsis patients. Although there is little doubt that effective therapeutic control of the sep sis syndrome could be achieved via appropriate modulation of the cells of the I mmune system, at the present time we do not have an immune therapeutic regimen w hich can singly be efficacious for the general population of patients with the s eptic complication. This implies that before an effective treatment of sepsis p atients, the patients must be identified, by some diagnostic procedure, as to wh ether they need an anti-pro-inflammatory therapy or an anti-anti-inflammatory th erapy. Thus, although immuno-therapy of sepsis remains promising, its efficacy a waits further investigative work particularly through clinical studies in sepsis patients.

  4. The Determination of the EDTMP Content by Complexometric Titration with Stan

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    10TheDeterminationoftheEDTMPContentbyComplexometricTitrationwithStandardZincSolution¥ChenSuzhenTheEDTMPcontentwasdeterminedby...

  5. A Brief History of the NPS Field Experimentation Program: Spanning STAN, TNT, and JIFX

    Science.gov (United States)

    2014-08-01

    subsequent Peer-to-Peer and traditional 802.11 topology Hi ba ndwidth, relia ble mesh network technology tha t TNT a dopted as the test bed...Commandos: Why AFSOC Needs a New Skyraider. 2011 JSOU and NDIA SO/LIC Division Essays (JSOU Report 11-4), pp 25-36. Klaus , C. and T. H. Chung (2011

  6. INVESTIGATION AND ASSESSMENT OF OCCUPATIONAL RISK ON THE METAL CUTTING MACHINE TOOL STAN

    Directory of Open Access Journals (Sweden)

    Jerzy Józwik

    2013-12-01

    Full Text Available The paper analyzes occupational risk at selected metal cutting machine tool stands (turning, milling, drilling and grinding stands and presents a set of recommendations formulated to minimize hazards at the above mentioned stands. The assessment is based on the analysis that allowed for selecting the best method of occupational risk assessment, measurements, observations, interviews and conclusions drawn from the obtained results. Occupational risk assessment forms were devised, what allowed for selecting appropriate action to increase the safety of selected machinery operators. The guidelines were assessed in terms of their practical implementation and expected utilitarian results.

  7. Research needs on food marketing to children. Report of the StanMark project.

    Science.gov (United States)

    Lobstein, T

    2013-03-01

    A series of meetings on the topic of children's exposure to the marketing of food and beverages was held between researchers and government officials based in Europe and the Americas during 2010-2011. The meetings resulted in a number of outputs, including observations from policy-makers on the types of evidence they needed to strengthen policy-making. Their observations on the definitions of a child, the specification of foods using nutrient profiling schemes, the types of media carrying marketing messages, and the related policy implementation problems, are summarised in this Short Communication. The paper highlights the need for research which can directly support policy-making and which can evaluate its effectiveness. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Stan liderom dlja samogo sebja / Mihkel Pärjamäe

    Index Scriptorium Estoniae

    Pärjamäe, Mihkel

    2007-01-01

    Liidriks olemise eri vormidest tänapäeval. Inimeste jagunemisest liidriteks ja mitteliidriteks. Mitteliidritest kui probleemist ühiskonnas. Domineerimisest kui omadusest, mis ei kuulu liidriomaduste hulka. Liidriomaduste arendamisest. Esimene samm liidriks olemise poole: hakata liidriks iseenda jaoks, st. kujundada visioon omaenda tulevikust, töötada välja strateegia ja hakata seda järjekindlalt ellu viima

  9. THE STATE OF AZADİSTAN(THE STATE, ESTABLİSHED IN IRAN 1920

    Directory of Open Access Journals (Sweden)

    Abbas KARAAĞAÇLI

    2013-08-01

    Full Text Available Towards the end of World War I, an important anti-colonialist and liberationist political and social movement emerged in the Azerbaijan Region in Iran. They have coalesced under the leadership of the revolutionist and intellectual venerable Sheikh Mohammed Hiyabâni, who has been among the leaders of the Iranian Democratic Party’s Regional Organization during the Constitutional Revolution (1908-1911 and one of the members of the first parliament. Their goal and aims were to contribute to the independence of Iran, and especially to resist the attempts and practices for dividing Iran between the Prime Minister Vosouq al-Dowleh and the British government. This article analyzes the emergence, continuation and fall of the Azadistan (Land of Freedom Movement.

  10. A p An atte proces empt t ss of da oward awada s stan awan b ...

    African Journals Online (AJOL)

    SAM

    2014-08-15

    Aug 15, 2014 ... samples with the highest characteristic dawadawan botso aroma as described by the .... to have a significant effect on the organoleptic characteristics of Bikalga .... cheese (Tavaria et al., 2002). Despite the increase in bitter ...

  11. Ribosome profiling: a Hi-Def monitor for protein synthesis at the genome-wide scale.

    Science.gov (United States)

    Michel, Audrey M; Baranov, Pavel V

    2013-01-01

    Ribosome profiling or ribo-seq is a new technique that provides genome-wide information on protein synthesis (GWIPS) in vivo. It is based on the deep sequencing of ribosome protected mRNA fragments allowing the measurement of ribosome density along all RNA molecules present in the cell. At the same time, the high resolution of this technique allows detailed analysis of ribosome density on individual RNAs. Since its invention, the ribosome profiling technique has been utilized in a range of studies in both prokaryotic and eukaryotic organisms. Several studies have adapted and refined the original ribosome profiling protocol for studying specific aspects of translation. Ribosome profiling of initiating ribosomes has been used to map sites of translation initiation. These studies revealed the surprisingly complex organization of translation initiation sites in eukaryotes. Multiple initiation sites are responsible for the generation of N-terminally extended and truncated isoforms of known proteins as well as for the translation of numerous open reading frames (ORFs), upstream of protein coding ORFs. Ribosome profiling of elongating ribosomes has been used for measuring differential gene expression at the level of translation, the identification of novel protein coding genes and ribosome pausing. It has also provided data for developing quantitative models of translation. Although only a dozen or so ribosome profiling datasets have been published so far, they have already dramatically changed our understanding of translational control and have led to new hypotheses regarding the origin of protein coding genes. Copyright © 2013 John Wiley & Sons, Ltd.

  12. T-wave oversensing and inappropriate shocks in implantable cardioverter def ibrillators

    Institute of Scientific and Technical Information of China (English)

    GAO Lei; LU Cai-yi; WANG Shi-wen; XUE Qiao; YAN Wei; ZHOU Sheng-hua; CHEN Rui; LIU Peng; ZHAI Jin-yue

    2010-01-01

    @@ The implantable cardioverter defibrillator (ICD) represents today the treatment and prophylaxis of choice for patients at risk for sustained ventricular tachyarrhythmias.1"6 Accurate sensing of ventricular tachyarrhythmias is a critical aspect of the function of ICD.

  13. DefEX: Hands-On Cyber Defense Exercise for Undergraduate Students

    Science.gov (United States)

    2011-07-01

    foundational concepts including the SDLC, system-level hardening, and code-level hardening, students examined the vulnerable application “ Madam Zora...for the vulnerability involves inputting a value that causes WHERE statements to evaluate to true. In the Madam Zora web application, a vulnerable...Hardening exercise, students identified and patched 22 system-level vulnerabilities exhibited by the Madam Zora VMware image. The Zora image distributed to

  14. Prognozowanie i ujmowanie przychodóww przedsiębiorstwie postawionymw stan upadłości likwidacyjnej

    Directory of Open Access Journals (Sweden)

    Janusz Czerny

    2010-03-01

    Full Text Available W upadłości likwidacyjnej przedsiębiorstw mamy do czynienia z dwoma stru-mieniami przychodów, a mianowicie z przychodami dotyczącymi zbycia przedsię-biorstwa lub jego składników majątku oraz z przychodami związanymi z ewentual-nym kontynuowaniem działalności: Przychody ze sprzedaży przedsiębiorstwa powinny być prognozowane głównie na podstawie informacji płynących z systemu rachunkowości, co stanowi tzw. sta-tyczny aspekt upadłości, natomiast jej aspekt dynamiczny jest związany z zakre-sem i czasem kontynuowanej działalności, o czym decyduje syndyk, mając na uwa-dze naczelny cel upadłości, jakim jest optymalne zaspokojenie roszczeń wierzycieli.

  15. BULGARIAN STATE AND THE TURKS (1878-1908 / BULGARİSTAN EMARETİ VE TÜRKLER (1878–1908

    Directory of Open Access Journals (Sweden)

    Assoc. Prof. Dr. Osman KÖSE

    2006-12-01

    Full Text Available Bulgaria emerged as an independent state in the Berlin treaty, which was signed after the 1877-1878 Ottoman-Russian war. After this, the Bulgarian administration began an anti-Muslim massacre campaign, like the Russians did before.The main aim of this article is to investigate the pressure, violence and massacres against the Muslim-Turks, organised by the Bulgarian gangs and the official administration. It is also proposed that the emigration of the Muslim-Turkish population began after this policy of violence.

  16. Nuclear and radiation techniques - state of art and development trends; Techniki jadrowe i radiacyjne - stan obecny oraz kierunki rozwoju

    Energy Technology Data Exchange (ETDEWEB)

    Chmielewski, A.G. [Institute of Nuclear Chemistry and Technology, Warsaw (Poland)

    1995-12-31

    The state of art and development trends of nuclear and radiation techniques in Poland and worldwide have been presented. Among them the radiometric gages, radiation technologies, radiotracer methods and measuring systems for pipeline and vessels, brightness control have been described and their applications in industry, agriculture, health and environment protection have been shown and discussed. 35 refs, 1 fig.

  17. Co nowego w leczeniu stanów zapalnych narządów płciowych kobiet?

    Directory of Open Access Journals (Sweden)

    Grzegorz Stachowiak

    2010-06-01

    Full Text Available Pelvic inflammatory disease (PID is a large group of inflammatory polymicrobial illnesses of female genitalorgans, mostly caused by Chlamydia trachomatis and Neisseria gonorrhoeae. When poorly treated it leads tosome serious distant side effects e.g. impaired fertility. Quinolones play an important role in the treatment ofPID, whereas newly introduced moxifloxacin is characterized by high therapeutic efficacy and safety.

  18. The state and prospect of nuclear energetics supply in fuel; Stan i perspektywy zaopatrzenia energetyki jadrowej w paliwo

    Energy Technology Data Exchange (ETDEWEB)

    Dembinski, W. [Institute of Nuclear Chemistry and Technology, Warsaw (Poland)

    1996-12-31

    The worldwide demand on nuclear fuel for energetics has been analysed on the base of data from the period 1988-1994. Production of nuclear fuels in different countries has been shown and compared for 1992, 1993 and 1994. The increasing deficit of uranium has been noticed from 1986. Alternative sources of nuclear fuels utilization, e.g. military origin uranium and plutonium recycling, have been also discussed. 4 refs, 4 figs, 2 tabs.

  19. Pluto in Hi-Def Note: There is debate within the science community as to whether Pluto should be

    Science.gov (United States)

    2008-01-01

    This image demonstrates the first detection of Pluto using the high-resolution mode on the New Horizons Long-Range Reconnaissance Imager (LORRI). The mode provides a clear separation between Pluto and numerous nearby background stars. When the image was taken on October 6, 2007, Pluto was located in the constellation Serpens, in a region of the sky dense with background stars. Typically, LORRI's exposure time in hi-res mode is limited to approximately 0.1 seconds, but by using a special pointing mode that allowed an increase in the exposure time to 0.967 seconds, scientists were able to spot Pluto, which is approximately 15,000 times fainter than human eyes can detect. New Horizons was still too far from Pluto (3.6 billion kilometers, or 2.2 billion miles) for LORRI to resolve any details on Pluto's surface that won't happen until summer 2014, approximately one year before closest approach. For now the entire Pluto system remains a bright dot to the spacecraft's telescopic camera, though LORRI is expected to start resolving Charon from Pluto seeing them as separate objects in summer 2010.

  20. The Decisions, Interventions, and Goals in ImplaNtable Cardioverter-DefIbrillator TherapY (DIGNITY) Pilot Study.

    Science.gov (United States)

    Kramer, Daniel B; Habtemariam, Daniel; Adjei-Poku, Yaw; Samuel, Michelle; Engorn, Diane; Reynolds, Matthew R; Mitchell, Susan L

    2017-09-22

    Implantable cardioverter-defibrillators (ICDs) are commonly implanted in older patients, including those with multiple comorbidities. There are few prospective studies assessing the clinical course and end-of-life circumstances for these patients. We prospectively followed 51 patients with ICDs for up to 18 months to longitudinally assess in terms of (1) advance care planning, (2) health status, (3) healthcare utilization, and (4) end-of-life circumstances through quarterly phone interviews and electronic medical record review. The mean age was 71.1±8.3, 74.5% were men, and 19.6% were non-white. Congestive heart failure was predominant (82.4%), as was chronic kidney disease (92%). At baseline, a total of 12% of subjects met criteria for major depression, and 78.4% met criteria for mild cognitive impairment. From this initial study cohort, 76% survived to 18 months and completed all follow-up interviews, 18% died, and 19% withdrew or were lost to follow-up. Though living will completion and healthcare proxy assignment were common (cumulative outcome at 18 months 88% and 98%, respectively), discussions of prognosis were uncommon (baseline, 9.8%; by 18 months, 22.7%), as were conversations regarding ICD deactivation (baseline, 15.7%; by 18 months, 25.5%). Five decedents with available data received shocks in the days immediately prior to death, including 3 of whom ultimately had their ICDs deactivated prior to death. We demonstrated the feasibility of prospective enrollment and follow-up of older, vulnerable ICD patients. Early findings suggest a high burden of cognitive and psychological impairment, poor communication with providers, and frequent shocks at the end of life. These findings will inform the design of a larger cohort study designed to further explore the experiences of living and dying with an ICD in this important patient population. © 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

  1. Pluto in Hi-Def Note: There is debate within the science community as to whether Pluto should be

    Science.gov (United States)

    2008-01-01

    This image demonstrates the first detection of Pluto using the high-resolution mode on the New Horizons Long-Range Reconnaissance Imager (LORRI). The mode provides a clear separation between Pluto and numerous nearby background stars. When the image was taken on October 6, 2007, Pluto was located in the constellation Serpens, in a region of the sky dense with background stars. Typically, LORRI's exposure time in hi-res mode is limited to approximately 0.1 seconds, but by using a special pointing mode that allowed an increase in the exposure time to 0.967 seconds, scientists were able to spot Pluto, which is approximately 15,000 times fainter than human eyes can detect. New Horizons was still too far from Pluto (3.6 billion kilometers, or 2.2 billion miles) for LORRI to resolve any details on Pluto's surface that won't happen until summer 2014, approximately one year before closest approach. For now the entire Pluto system remains a bright dot to the spacecraft's telescopic camera, though LORRI is expected to start resolving Charon from Pluto seeing them as separate objects in summer 2010.

  2. Experience with system safety approach in railroad technology in Europe and Far East and in aerospace technology; Erfahrungen mit Ansaetzen zur Systemsicherheit in der Bahntechnik in Europa und im Fernen Osten sowie in der Raumfahrttechnik

    Energy Technology Data Exchange (ETDEWEB)

    Schaebe, H. [Technischer Ueberwachungs-Verein InterTraffic GmbH, Koeln (Germany). Inst fuer Software, Elektronik, Bahntechnik

    2000-07-01

    Safety analyses play an important role in assessing railroad technology, especially in the light of the CENELEC Standards EN 50126 and EN 50129. The European approach to system safety is based on the EN standards, whereas in Asia we had to deal with the British DEF-STAN standards. Although the approach is inspired by the European one, in the particular case specific changes have been observed. On the other hand there is a long tradition in space technology which is based in Europe on the PSS standards edited by ESA and the ECSS standards which have followed them. The EN series of standards focuses on the safety case approach. It must be shown that the system is sufficiently safe. Analyses as hazard analyses, Failure Modes, Effects, and Criticality Analyses and Risk analyses are used together with other methods. An important problem is the definition of the target for safety characteristics. We present experience from assessment of driverless people mover systems. On the other hand we have performed analyses for locomotives that have been supplied for the North East Line and the Changi Airport Line of the metro in Singapore. Here, the approach was based on the British DEF-STAN series. In this particular project, however, the approach led to a larger number of exhaustive analyses for a workshop locomotive which is equipped with a driver. In aerospace, often a large number of analyses is necessary. However, the analyses are adopted regarding their number and level of detail depending on the system. As an example, test facilities used at ESTEC (technical centre of the European Space Association) are presented. The experience gained in different areas of technology and in different geographic areas are compared. It will be shown how the EN standards can be used effectively. (orig.) [German] Sicherheitsanalysen spielen eine wichtige Rolle bei der Beurteilung der Bahntechnik, insbesondere im Rahmen der CENELEC Normen EN 50126 und EN 50129. Der Europaeische Ansatz der

  3. Radiographic picture of hypertrophic pseudoarthrosis of the leg and condition of arterial vascularity of the limb; Obraz radiologiczny hipertroficznych stawow rzekomych goleni a stan tetniczego ukrwienia konczyny

    Energy Technology Data Exchange (ETDEWEB)

    Konarski, K.; Trubisz, L.; Piotrowski, W. [Szpital im. J. Jonstona, Lublin (Poland)

    1993-12-31

    Weber and Cech classification of pseudoarthroses allowing for assessment of its viability is presented. An angiography was found to be useful in determination of pseudoarthrosis viability. Femoral arteriography has been performed in 18 cases of hypertrophic pseudoarthroses assessed as viable on the basis of radiographic examination. In 13 cases arterial injuries indicating diminished biological reactivity of pseudoarthrosis of the leg were found. (author). 7 refs, 3 figs, 1 tab.

  4. Niedualna uważność a stan samādhi w kontekście badań neurofenomenologicznych

    Directory of Open Access Journals (Sweden)

    Piotr PŁANETA

    2016-12-01

    Full Text Available The aim of this paper is to compare various meditative states, such as Buddhist dhyāna‑s, yogic nirbīja samādhi and nondual awareness (Tib. gñis‑med. The primary source texts I refere to are Yogasūtras of Patañjali, Ānāpānasmṛtisūtra (MN 118, Samādhisūtra (AN 4.41, The Tibetan Yogas of Dream and Sleep. I also discuss some relevant claims of contemporary empirical studies. First, I define the key terms used in Eastern meditation studies as well as in neurophenomenology, a contemporary method applied to examining the meditative states of mind, such as samādhi, dhyāna, and śamatha. Inspired by Shinzen Young, I distinguish three groups of meditative states that might be identified with nondual awareness. These three groups are: (1 the second, the third and fourth Buddhist dhyāna being equivalent to nirvicāra samādhi and nirānanda samādhi in the classical Indian yoga; (2 nirbīja samādhi and (3 nondual awareness, typical to the Mahayāna contemplative traditions. I explain why we can recognize each of the above states as nondual awareness and how they differ from each other. Then, I make a comparison between meditation practice explained in Ānāpānasmṛtisūtra and nondual awareness presented in the Tibetan Buddhism. Besides, I discuss the above kinds of mental states in terms of recent neurophenomenological findings. While doing so, I am trying to demonstrate that our understanding of meditation can benefit from the empirical studies which help us to objectify this kind of subjective experience, to some degree, if they are given an adequate place in our study.

  5. Przełączanie kodów czy kod mieszany? Aktualny stan bilingwizmu staroobrzędowców w regionie suwalsko-augustowskim

    Directory of Open Access Journals (Sweden)

    Michał Głuszkowski

    2015-07-01

    Старообрядцы в сувальско-августовском регионе образуют двуязычную общину. Они попеременно пользуются польским языком и русским диалек­том. В их речи наблюдаются многочисленные случаи переключения кодa, т.е. смены языка высказывания между предложениями, на стыке придаточных предложений и других функционально значимых синтаксических единиц, а также смешение кодов, т.е. смены языка внутри меньших единиц. Согласно положениям П. Ауэра, смешение кодов может привести к образованию сме­шанного кода (fused lect. Данная статья является попыткой определить со­стояние билингвизма польских старообрядцев, а также дать ответ на вопрос о возможном образовании смешанного кода на основании русского говора. Анализ опирается на языковые и социолингвистические данные, собранные во время научных экспедиций в старообрядческих местностях северо-восточной Польши в 1999–2011 гг.

  6. STUDIES ABOUT DEDE KORKUT IN TURKMENİSTAN TÜRKMENİSTAN’DA DEDE KORKUT HAKKINDA YAPILAN ÇALIŞMALAR

    Directory of Open Access Journals (Sweden)

    İhsan KALENDEROĞLU

    2011-01-01

    Full Text Available Dede Korkut and the Dede Korkut legends which have been formed around his name have a great significance in bearing the past values of Turkish history and culture and in joining together the Turkish world in many ways.Today, many studies have been done on Dede Korkut legends which we know existed in different regions Turkish people lived in. Dede Korkut legends which were collected in the post-Islamic Anatolia region, Azerbaijan and Turkmenistan have attracted great attention from researchers.Turkmenistan is one of the regions where these studies have been intensive. Several studies were done in Turkmenistan on Dede Korkut legends especially during the Bolshevist era despite the difficulties faced and a noticable increase has been seen in these studies since 1985. In this study, we will try to explain the studies on Dede Korkut legends carried out by Turcologists working on Dede Korkut legends and Turkmen folklore. Dede Korkut ve onun ismi etrafında şekillenen Dede Korkut destanları, Türk tarihinin ve kültürünün geçmişe ait değerlerini taşıma, Türk dünyasını birçok yönden kaynaştırma noktasında çok büyük önem taşımaktadır. Günümüzde, Türklerin yaşadığı farklı coğrafyalarda anlatıldığını bildiğimiz Dede Korkut destanları ile ilgili pek çok çalışma yapılmıştır. Özellikle, İslamiyet sonrası Anadolu coğrafyasında, Azerbaycan ve Türkmenistan’da derlenmiş Dede Korkut destanları araştırmacıların yoğun ilgisini çekmiştir. Bu araştırmaların yoğunlaştığı yerlerden birisi de Türkmenistan’dır. Türkmenistan’da Dede Korkut destanları ile ilgili özellikle, Bolşevik dönemde karşılaşılan güçlüklere rağmen birtakım çalışmalar yapılmış, ağırlıklı olarak 1985 yılından itibaren Dede Korkut ile ilgili yapılan çalışmalarda gözle görülür bir artış olmuştur. Bu makalede, Dede Korkut destanlarının Türkmen folkloru için önemini vurgulayarak; Türkmenistan’da ve Rusya’da Türkmen folkloru ile ilgili çalışmalar yapan Türkologların, Dede Korkut destanları hakkında yaptıkları çalışmaları, eldeki kaynaklar ölçüsünde açıklamaya çalışacağız.

  7. Polszczyzna na Mohylewszczyźnie – przeszłość i stan obecny (raport z badań terenowych

    Directory of Open Access Journals (Sweden)

    Małgorzata Ostrówka

    2015-07-01

    Full Text Available Polish language in the Mohylew region – the past and present (the report on field research The research in the Mohylew region is a continuation of research concerning the language of Catholics in former North-Eastern Borderland. The work contains an outline of the history of the Mohylew region including the history of the Catholic Church, education and functioning of Polish in this land. Besides Mohylew the following places were visited: Czausy, Faszczówka and Bezczynne where parishes are being revived. Evangelisation is in Belorussian and only in Mohylew one Holy Mass is in Polish every day. Conclusions: The Polish language in the Mohylew region has been functioning since 16th century what is confirmed in Mohylew town chronicles grave inscriptions in local Polish Cemetary. It has also been, excluding Jesuit parishes (Jesuits evangelised in the language of a given nationality, wrote catechisms and grammars the language of prayers and lithurgy. The result of the progress of russification was that the range of its use narrowed down. The next stage (20’s and 30’s of 20th century of the fight with the Church and religion led to interrupting passing the Polish language even in those families where it survived throughout former stages. In this way the Polish tradition was interrupted. At present it is very difficult to meet people using the old local Polish language. The Polish with regional features can be heard with those people who came to Mohylew after the World War II. There is also another quality: the language learned at school or courses. The Polish language is generally idiolectally diverse, its shape depends on the degree of fluency in Polish. On the basis of reviving catholicism and the Polish language with numerous young people who discovered their roots there is a process of reconstructing the Polish identity.   Польский язык на Могилёвщине – прошлое и современность (отчёт по полевым исследованиям Полевые исследования на Могилёвщине являются продолжением проводимых авторами исследований языка католиков на бывших северо-восточных рубежах Польши. В статье представлен краткий очерк истории Могилёвщины, католической церквы, просвещения на польском языке и функционирования польского языка на исследуемой территории. Кроме Могилёва авторы статьи посетили Чаусы, Фащевку и Бесчине. В этих местностях возрождаются католические приходы. Евангелизация и богослужения ведутся на белорусском языке. Только в Могилёве ежедневно одна месса происходит на польском языке. Выводы: Польским языком на Могилёвщине пользовались с XVI века, что подтверждают городские хроники и надписи на местном Польском кладбище. Кроме того он был (за исключением приходов, которые вели иезуиты, которые вели римскокатолическое вероучение на национальных языках языком молитвы и литургии. По мере усиливания руссификации во время разделов Польши, использование польского языка уменьшалось. Очередной период (20-е и 30-е годы ХХ века борьбы с католической церковью и религией стали причиной прекращения передачи польского языка даже в тех семьях, в которых он сохранился в предыдущий период. Одновременно прекратилась польская традиция. В настоящее время трудно найти людей, говорящих на давнем местном польском языке. Польский язык, насыщенный региональными диалектными чертами, встречается ещё у лиц, которые прибыли на Могилёвщину после второй мировой войны. Мы обнаружили ещё одну разновидность польского языка – это язык выученный в школе и на языковых курсах. В общем польский язык на Могилёвщине сильно дифференцирован в зависимости от индивида, а его качество от степени присвоения данного кода. Опираясь на возрождающийся католицизм и польский язык у многих молодых людей, которые обнаружили свои польские корни, наступает процесс реконструкции польского самосознания.

  8. Test and Evaluation of Category III ILS Ground Guidance Equipment ’STAN-37 Localizer Tests at NAFEC on R/W-4’.

    Science.gov (United States)

    Aviation Facilities Experimental Center (NAFEC), Atlantic City, New Jersey. These included conformance to ICAO Annex 10 Category 3 ILS specifications...characteristics met ICAO specifications, and that monitor executive action occurs as required when faults are present. System availability sufficient for

  9. POSSESIVE CONSTRUCTIONS IN AFGHANISTAN TURKMEN TEXTS AFGANİSTAN TÜRKMENCESİ METİNLERİNDEKİ EKSİZ AD TAMLAMALARI

    Directory of Open Access Journals (Sweden)

    Rıdvan ÖZTÜRK

    2010-01-01

    Full Text Available Generally, Afghanistan Turkmen is compatible with Turkmenistan Turkmen. It hasn’t been standardization in literary yet, so it reflects some features its own unique. One of these features is possesive constructions without suffixes. Possesive constructions without suffixes were researched in terms of constituted permanent nouns entitle “goşma noun” (combined noun, compound word in Turkmen language resources. It wasn’t dealed with possesive constructions which did not constitute permanent nouns and was constututed by removing of third person possesive suffix. In this article, it was researched into possesive constructions without suffix determined in Turkmen puplished works in Afghanistan. Afganistan Türkmencesi genel itibariyle Türkmenistan Türkmencesine uygundur. Ancak yazılı geleneğe ve standartlaşmaya henüz geçememiş olması, onun kendisine has bazı sıra dışı özellikleri eselere yansıtmasını sağlamıştır. Bu özelliklerden birisini eksiz ad tamlamaları oluşturmaktadır. Eksiz ad tamlamaları, Türkmence dil bilgisi kaynaklarında “goşma ad” (birleşik ad, birleşik kelime başlığı altında kalıcı adlar oluşturmaları yönü ile incelenmiştir. İyelik üçüncü şahıs ekinin düşürülmesi ile oluşturulan ve kalıcı ad konumuna geçmemiş ad tamlamalarına ise temas edilmemiştir. Bu yazıda, Afganistan Türkmencesi ile yayımlanmış eserlerden tespit ettiğimiz eksiz ad tamlamaları incelenmiştir.

  10. NCBI nr-aa BLAST: CBRC-CBRE-01-1302 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CBRE-01-1302 gb|ABJ74629.1| stan [Drosophila miranda] gb|ABJ74630.1| stan [Drosophila miran...da] gb|ABJ74631.1| stan [Drosophila miranda] gb|ABJ74632.1| stan [Drosophila miranda] gb|ABJ746...33.1| stan [Drosophila miranda] gb|ABJ74634.1| stan [Drosophila miranda] gb|ABJ74635.1| stan [Drosophila miran...da] gb|ABJ74637.1| stan [Drosophila miranda] gb|ABJ74638.1| stan [Drosophila miran...da] gb|ABJ74641.1| stan [Drosophila miranda] gb|ABJ74642.1| stan [Drosophila miranda] gb|ABJ74643.1| stan [Drosophila miran

  11. Draft Title 40 CFR 191 compliance certification application for the Waste Isolation Pilot Plant. Volume 5: Appendices D and D, DEF, FAC

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-31

    This plan serves to describe the objectives of decommissioning for the Waste Isolation Pilot Plant (WIPP), identifies the elements necessary to accomplish the decommissioning, and defines the steps to execute those elements in a safe and environmentally sound manner. The plan provides a strategy for progressing from the final actions of the Disposal Phase, through the Decontamination and Decommissioning Phase, and into the initiation of the Long-Term Monitoring Phase. This plan describes a sequence of events for decontamination of the WIPP facilities and structures used to manage and contain TRU and TRU mixed waste during the receipt and emplacement operations. Alternative methods of decontamination are provided where practical. The methods for packaging and disposal of the waste generated (derived waste) during this process are discussed. The best available technology at the time of this plan`s development, may become outmoded by future technology and alternative strategies. If alternative technologies are identified the affected stakeholder(s), the Secretary of the Interior and the State will be consulted prior to implementation.

  12. Selection on length mutations after frameshift can explain the origin and retention of the AP3/DEF-like paralogues in Impatiens

    NARCIS (Netherlands)

    Janssens, S.; Viaene, T.; Huysmans, S.; Huysmans, S.; Smets, E.F.; Geuten, K.

    2008-01-01

    Evolution of class B genes through gene duplication has been proposed as an evolutionary mechanism that contributed to the enormous floral diversity. Frameshift mutations are a likely mechanism to explain the divergent C-terminal sequences of MIKC gene subfamilies. So far, the inferences for framesh

  13. Production of jet fuel using heavy crude oil; Producao de combustiveis de aviacao a partir de petroleos pesados

    Energy Technology Data Exchange (ETDEWEB)

    Om, Neyda [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Escola de Quimica; Cavado, Alberto; Reyes, Yordanka [Centro de Pesquisas do Petroleo, Cidade de Havana (Cuba); Dominguez, Zulema [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia (COPPE)

    2004-07-01

    The production of heavy crude oils increased in the last years in the world. Crude oils with high density, viscosity, acidity and sulfur, nitrogen, metals and asphaltenes contents, by the others hand, low stability and low product quality. The challenger of many refiners is find solutions to refine the heavy crude oils, and produce fuels with certify quality, such as Jet Fuel. The principal aviation technique on the world work with gas turbines engines feted for jet fuel (JET A1). The quality specifications of this fuel are establish by International Norms: ASTM-1655, DEF STAN 91-91-3 (DERD 2494) and joint Fuelling System Check List. The world technologies to obtain jet fuel from mixtures of heavy crude oil with middle crude oils are: Atmospheric distillation, with a posterior hydrogenation and finally the additivation. Studies carried out have demonstrates that the Cubans heavy crude oils is characterized for having API less than 10, raised viscosity, high sulfur content (>6%) and asphaltenes content (more than 15%). These properties provide to its derivatives of low quality. This paper define the characteristic of Cuban heavy crude oil, the technology and operational conditions to produce jet fuel (Jet A1) and the quality of fuel produced. (author)

  14. Quality Management System for Defence Aeronautical Industry

    Directory of Open Access Journals (Sweden)

    K.T. Thomas

    2006-01-01

    Full Text Available Indian defence aeronautical industry, while becoming a global business, demands a largeeffort to monitor quality management system (QMS and to ensure quality of aeronauticalproducts. An effort is being made to look for an effective QMS for aeronautical industry in India,which will also meet the requirements of the regulatory authority.The essential features of an effective QMS are described and compared with the presentlyavailable QMS standards for defence aeronautical industry such as QCSR: 2002 (DGAQA, India,Def Stan (MOD, UK, ISO, AQAPs (NATO and Society of Automotive Engineers (SAE aerospacestandards (AS. Evolution, relevance and review of the existing standards, to meet the requirementsof the industry as well as regulatory authority, have been made to bring out the special featuresand differences. The study leads to the most acceptable standard of SAE-AS-9100 (Rev B.With suitable modifications to include regulatory requirement of assistance for governmentquality assurance in the standard, when complied with, it will fully meet the QMS requirementsof the Indian defence aeronautical supply organisations as well as the requirements of theregulatory authority. Minor reorientation of the regulatory functions and inclusion of the QMSin the defence aeronautical supply orders are also suggested.

  15. 管道混合输送油品对喷气燃料性质的影响%Impact on Jet Fuel Property of Multi-product Pipeline

    Institute of Scientific and Technical Information of China (English)

    魏大明; 李明

    2007-01-01

    在利用长距离管道输送喷气燃料、石脑油、汽油和柴油等成品油时,为保障喷气燃料的油品质量,喷气燃料中最多只能混入质量分数≤0.75%的石脑油和汽油,在保证闪点合格的前提下,按照Jet A-1标准(ASTM D1655和DEF STAN91-91)喷气燃料指标仍能合格.喷气燃料中只能混入质量分数<0.2%的柴油.所以在实际输送工作中,喷气燃料中混入的汽油、柴油和石脑油量均需严格控制,应小于实验室静态试验理论的计算值.

  16. HİNDİSTAN BHOPAL EMÎRİ VE MÜFESSİR SIDDIK HASAN HAN'IN OSMANLI DEVLETİ İLE İLİŞKİSİ

    OpenAIRE

    2010-01-01

    Bu çalışma Osmanlı Devleti'nin sınır ötesi İslamı unsurlarlaolan ilişkilerine örnek sadedinde, kısaca Hindistan Müslümanlarıve Hind Bhopal Emiri ve Müfessir Sıddık Hasan Han'la olan ilişkilerikonu edinmektedir. ABSTRACTIn this study, the relationship between Ottornan State andİslaroie raees in foreign eountries has been diseussed. Esexsample, Muslirns of India in short, and especially the BhopalErneer and eomrnentator on the Qur'an Sıddık Hasan Khan....

  17. Autumn school. Radiation of medical materials. Actual state and development perspectives, new international regulations. 18-19 November 1993; Szkola jesienna. Sterylizacja radiacyjna sprzetu medycznego: stan aktualny i perspektywy rozwoju, nowe przepisy miedzynarodowe. 18-19 listopad 1993

    Energy Technology Data Exchange (ETDEWEB)

    1993-12-31

    A critical review of medical sterilization methods commercially used has been done. On this base scientific, technical and production aspects of radiation sterilization technology have been discussed. Especially the process control, dosimetric methods used and microbiological problems were described. All legal aspects, Polish and international regulations and licensing procedures for different kinds of medical materials have been also shown. The development of radiation sterilization in Poland in respect to dressings, tissue transplants, cosmetics, tissue transplants, cosmetics, pharmaceutics, packing materials as well as other radiation technologies has been presented.

  18. Molestowanie seksualne przed uzyskaniem pełnoletności a stan zdrowia i funkcjonowanie społeczne osób uzależnionych od opioidów leczonych substytucyjnie

    Directory of Open Access Journals (Sweden)

    Sylwia Fudalej

    2016-03-01

    Conclusion: The results of our study suggest the potential benefit to multifaceted treatment approach, which should specifically address sexual abuse and suicidal behaviour in opioid-dependent patients.

  19. AN ASSESSMENT ON UPPER KARABAKH ISSUE AND TURKEY-ARMENIA RELATIONS YUKARI KARABAĞ SORUNU VE TÜRKİYE-ERMENİSTAN İLİŞKİLERİ ÜZERİNE BİR DEĞERLENDİRME

    Directory of Open Access Journals (Sweden)

    Mustafa GÖKÇE

    2011-01-01

    Full Text Available The Upper Karabakh issue has been one of the most important issues affecting stability in Caucasia after the fall of Soviets. In Karabakh which is a historical Turkish native country, Armenians were placed as from the Russia of Tsarizm and a region where the Armenians have a high population was created within the Azerbaijan lands. This field which is a mountainous region located in the upper parts of Karabakh is called Upper Karabakh or Nagorno- Karabakh. Due to the activities of Armenians settling in the region, conflicts started to happen as of the 19th Century and when came to 20th Century, these conflicts confronted us as a serious issue. During the fall of Soviets that have continued the population policies of the Russia of Tsarizm, the conflicts in Azerbaijan lands awaked again. In Upper Karabakh issue, Turkey has been seen as an important actor that would affect the process because of its historical and cultural bonds with Azerbaijan and also being a border neighbour to the region. While its military intervention in the region was discussed at the process when conflicts were experienced, Turkey has followed a more cautious policy and made efforts for solving the problem through peaceful ways at an international platform. Turkey, which is among the countries first recognizing the independencies of Azerbaijan and Armenia, has given importance to the Upper Karabakh issue in its relations with these countries. Being on the side of Azerbaijan, Turkey has closed the border of Armenia as a sanction. Within this scope, the relations started with Armenia were broken in a short time. And the steps taken towards normalizing the relations recently didn’t give any positive results due to the historical problems with Armenia and the continuation of occupancy in Azerbaijan lands. Yukarı Karabağ sorunu, Sovyetlerin çöküşünden sonra Kafkasya’daki istikrarı etkileyen sorunların başında yer almaktadır. Tarihi bir Türk yurdu olan Karabağ’a Çarlık Rusyası döneminden itibaren Ermeniler yerleştirilerek Azerbaycan toprakları içinde Ermenilerin yoğunlukta olduğu bir bölge oluşturulmuştur. Karabağ’ın üst kısımlarındaki dağlık bir yer olan bu bölge; Yukarı Karabağ veya Dağlık Karabağ olarak adlandırılmaktadır. Bölgeye yerleşen Ermenilerin faaliyetleri nedeniyle 19. yüzyıldan itibaren çatışmalar yaşanmaya başlamış, 20. asırda ise bu çatışmalar ciddi bir sorun olarak karşımıza çıkmıştır. Çarlık Rusyası’nın nüfus politikalarını devam ettiren Sovyetlerin çöküşü sırasında Azerbaycan topraklarındaki çatışmalar tekrar canlanmıştır. Yukarı Karabağ meselesinde Türkiye hem Azerbaycan ile olan tarihi ve kültürel bağları hem de bölgeye sınır komşusu olması nedeniyle sürece etki edecek önemli bir aktör olarak görülmüştür. Çatışmaların yaşandığı süreçte bölgeye askeri müdahalesi tartışılırken Türkiye, temkinli bir politika izleyerek sorunun uluslararası platformda barışçı yollardan çözülmesi için çalışmıştır. Azerbaycan ve Ermenistan’ın bağımsızlıklarını ilk tanıyan ülkeler arasında olan Türkiye, bu ülkelerle olan ilişkilerinde Yukarı Karabağ meselesini önemsemiştir. Azerbaycan tarafının yanında yer alan Türkiye, bir yaptırım olarak Ermenistan sınırını kapatmıştır. Bu çerçevede Ermenistan ile başlayan ilişkiler uzun sürmeden kesilmiştir. Son dönemde ilişkilerin normalleşmesi yolunda atılan adımlar da Ermenistan ile olan tarihi sorunlar ve Azerbaycan topraklarındaki işgalin devam etmesi nedeniyle olumlu bir netice vermemiştir.

  20. Dokąd zmierza informacja? - czyli co oferuje biblioteka, a czego oczekuje użytkownik. Stan obecny i rozwój informacji naukowej w bibliotekach uczelni technicznych

    OpenAIRE

    2006-01-01

    The paper presents theoretic assumptions and practical applications of library information services. It depicts transformations of information sources and forms of making them available as well as changes of users’ demands for specific types of information. New technologies cause changes in former organisation of presenting scientific information. More and more often science information is being decentralized, it is provided not only in currently acting Science Reference Divisions, Science In...

  1. Ostry trip po DMT czy bardo umierania? : potencjał kognitywnych teorii filmu na przykładzie analizy zmienionych stanów świadomości w "Enter the Void" Gaspara Noé

    OpenAIRE

    Piskorska, Anna

    2016-01-01

    This article shows how films can be used as contemporary research tools in order to get to know people's innerstates, with particular focus on changed states of consciousness such as psychedelic experience and near-death experience. On the basis of cognitive film theory (presented by Tzvetan Todorov, Edward Branigan, David Bordwell and Kristin Thompson) that goes beyond aesthetic reception of a film and studies the structures of cognition, the author of the article makes an attempt to analyze...

  2. Stan i możliwości rozwoju turystyki rowerowej w parkach narodowych Lubelszczyzny / State and development of bicycle tourism in the national parks of the Lublin Region

    Science.gov (United States)

    Kozieł, Marcin

    2012-12-01

    The article presents the results of surveys sent to managers directly responsible for bicycle tourism in the Polesie National Park and Roztocze. Based on the answers to the questionnaire survey the author of the paper presented information on the rules of access to national parks, tourist sites of the bicycle tourists, quality, functionality and the degree of difficulty and the number of available routes, and the discipline of cycling tourists moving along the designated routes. The managers were asked to determine how the national parks are going to promote cycling and what actions they intend to take (or not) in the near future, managing the national parks. During the field research there have been evaluated the two bike trails (bike trail "Mietiułka" in the Polesie National Park and the bicycle path "Zwierzyniec - Florianka - Górecko Stare" in the Roztocze National Park). Special attention has been paid to: the state of tourism development, marking and passable routes (the type and condition of surface) set out.

  3. Analysis of Issues and Measures in the Teaching Course of Nursing Stan-dardized Training Students%刍议在护理规培生带教过程中的问题及措施

    Institute of Scientific and Technical Information of China (English)

    蔡春艳; 张艳红

    2016-01-01

    随着医学的飞速发展,对护理规培生的规范化的培训也越来越关注,护理规培生规范化培训是医学生成长为合格护士的一条很好的途径。该文对护理规培生在培训过程的问题进行了深刻的分析,并对此提出了一些解决的措施。%With the rapid development of medicine, people pay more and more attention to the standardized training for nursing standardized training students, and the standardized training of nursing standardized training students is a good way for medical students to become a qualified nurse. The paper deeply analyzes the issues of nursing standardized training stu-dents in the training process and puts forwards some measurements.

  4. Exploration on the Guiding Role of Editors in the Stan-dardization of Scientific Papers%探索编辑在科技论文规范化中的引导作用

    Institute of Scientific and Technical Information of China (English)

    李小波

    2015-01-01

    科技论文是科学技术人员或其他研究人员在科学实验的基础上,按照各个科技期刊的要求进行电子和书面的表达.科技论文具有创新性、学术性、科学性.创新性是指能在科学理论或实践上获得新的进展;学术性是科技论文的主要特征,能充分揭示事物发展、变化的客观规律;科学性是指论文内容必须具有真实、客观等特点,论文语言必须准确规范,条理分明[1].为加强科技论文规范化,我们必须提高其编辑加工水平,使科技论文变得更加完善.%Scientific papers are electronic and written expressions based on the scientific experiments of scientific technicians and other researchers in accordance with the requirements of various scientific periodicals. Scientific papers are innovative, academic and scientific. Innovation means that new developments can be achieved in scientific theory and practice;academics is the main feature of scientific papers and can fully reveal the objective rules of the development and change of things; scientificalness means that the contents of papers must be real and objective, and the language of papers must be accurate, standardized and highly logical. To enhance the standardization of scientific papers, we must improve the level of editors' creation, so as to make scientif-ic papers perfect.

  5. 嗜麦芽寡养单胞菌外排泵SmeDEF的相关研究%Study of the SmeDEF efflux pump in Stenotrophomonas maltophilia

    Institute of Scientific and Technical Information of China (English)

    王佳嫄; 徐浩彤; 李刚; 贾伟

    2015-01-01

    目的 了解临床分离嗜麦芽寡养单胞菌对临床常用抗菌药物的耐药情况,并探讨氟喹诺酮类耐药与外排泵基因之间存在的关系.方法 收集2012年1月-12月宁夏医科大学总院及其附属心脑血管病医院住院患者标本中分离得到的嗜麦芽寡养单胞菌114株,采用琼脂平皿二倍稀释法测定7种抗菌药物对实验菌株的最低抑菌浓度(MIC),同批测定选择诺氟沙星、环丙沙星、氧氟沙星3种抗菌药物加入外排泵抑制剂(利血平)后抗菌药物的MIC值.应用荧光定量PCR法检测外排泵耐药基因表达水平.结果 114株嗜麦芽寡养单胞菌有37株对诺氟沙星耐药,25株对环丙沙星耐药,15株对氧氟沙星耐药.加入泵抑制剂后筛选得到19株外排泵阳性株.2株泵阳性的耐药株均有smeD和smeF的高表达.结论 用外排泵抑制剂可不同程度逆转嗜麦芽寡养单胞菌的耐药性.嗜麦芽寡养单胞菌临床菌株SmeDEF外排泵的表达强弱与其耐药性有关.

  6. 嗜麦芽窄食单胞菌临床株的多重耐药外排泵的研究%Stenotrophomonas maltophilia SmeDEF efflux pump and the regulation mechanism

    Institute of Scientific and Technical Information of China (English)

    孙二琳; 宋诗铎; 祁伟; 郭庆兰

    2004-01-01

    目的研究嗜麦芽窄食单胞菌临床株外排泵SmeDEF的表达与耐药的关系及其表达调控. 方法琼脂稀释法检测嗜麦芽窄食单胞菌对抗生素敏感性并检测泵抑制剂的作用,提取临床菌的RNA进行smeD的RT-PCR扩增.提取DNA进行smeT片段的PCR扩增,扩增产物进行序列分析. 结果随机选取的6株嗜麦芽窄食单胞菌均有扩增产物.SmeT的N端氨基酸序列相当保守,smeD-smeT间区测序发现耐药且泵抑制阳性株基因序列与敏感株明显不同.推测与耐药有关的突变出现在smeT的-82~-165区间. 结论嗜麦芽窄食单胞菌临床株SmeDEF外排泵的表达强弱与其耐药性有关.smeDEF基因的表达可能与调控基因间区的变化有关.

  7. Study of antibiotic induction expression of SmeDEF efflux pump in Stenotrophomonas maltophilia%嗜麦芽寡养单胞菌临床株外排泵SmeDEF诱导表达的研究

    Institute of Scientific and Technical Information of China (English)

    孙二琳; 宋诗铎; 祁伟; 王玉宝

    2007-01-01

    目的 了解嗜麦芽寡养单胞菌的临床株H407和A26在抗菌药物诱导下耐药性和SmeDEF外排泵表达的变化.方法 比较有或无环丙沙星诱导时嗜麦芽寡养单胞菌H407和A26的MIC和EOP值,对在亚抑菌浓度环丙沙星中培育后的菌株smeD基因进行RT-PCR扩增,监测表达水平.结果 对抗生素敏感的H407和A26泵抑制呈阳性或阴性反应.2株菌经环丙沙星过夜孵育后测得的对氯霉素和环丙沙星MIC值比正常非诱导时的MIC值高1~3个稀释度;加泵抑制剂CCCP后,2个诱导株的MIC值均有明显下降.H407和A26经环丙沙星诱导后氯霉素的EOP分别有4.8和近4倍的增长.2株菌在抗菌药物过夜和指数期添加生长时smeD的mRNA明显高于无抗菌药物培养株.结论 2株嗜麦芽寡养单胞菌临床株在环丙沙星诱导下对部分抗菌药物的耐药性提高,与SmeDEF泵的诱导表达增加有关.

  8. Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding dEF1

    Science.gov (United States)

    Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an estrogen receptor-enhanced cell line when treated with estrogen. This promoter-enhancer also binds unidentified proteins that recognize a consens...

  9. 78 FR 63267 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of No Objection to...

    Science.gov (United States)

    2013-10-23

    ... econometric models underlying STANS, time series of proportional changes in implied volatilities for a range... conditions;'' and ``use risk-based models and parameters to set margin requirements.''...

  10. Polymorphic segmental duplications at 8p23.1 challenge the determination of individual defensin gene repertoires and the assembly of a contiguous human reference sequence

    Directory of Open Access Journals (Sweden)

    Loncarevic Ivan F

    2004-12-01

    Full Text Available Abstract Background Defensins are important components of innate immunity to combat bacterial and viral infections, and can even elicit antitumor responses. Clusters of defensin (DEF genes are located in a 2 Mb range of the human chromosome 8p23.1. This DEF locus, however, represents one of the regions in the euchromatic part of the final human genome sequence which contains segmental duplications, and recalcitrant gaps indicating high structural dynamics. Results We find that inter- and intraindividual genetic variations within this locus prevent a correct automatic assembly of the human reference genome (NCBI Build 34 which currently even contains misassemblies. Manual clone-by-clone alignment and gene annotation as well as repeat and SNP/haplotype analyses result in an alternative alignment significantly improving the DEF locus representation. Our assembly better reflects the experimentally verified variability of DEF gene and DEF cluster copy numbers. It contains an additional DEF cluster which we propose to reside between two already known clusters. Furthermore, manual annotation revealed a novel DEF gene and several pseudogenes expanding the hitherto known DEF repertoire. Analyses of BAC and working draft sequences of the chimpanzee indicates that its DEF region is also complex as in humans and DEF genes and a cluster are multiplied. Comparative analysis of human and chimpanzee DEF genes identified differences affecting the protein structure. Whether this might contribute to differences in disease susceptibility between man and ape remains to be solved. For the determination of individual DEF gene repertoires we provide a molecular approach based on DEF haplotypes. Conclusions Complexity and variability seem to be essential genomic features of the human DEF locus at 8p23.1 and provides an ongoing challenge for the best possible representation in the human reference sequence. Dissection of paralogous sequence variations, duplicon SNPs ans

  11. VOCABULARY OF XVI. CENTURY TURKISH IN “ŞERH-I DIBÂCE-I GULISTAN” BY RUSDÎ RÜŞDÎ'NİN ŞERH-İ DİBÂCE-İ GÜLİSTAN'INDA XVI. YÜZYIL TÜRKÇESİNİN SÖZ VARLIĞI

    Directory of Open Access Journals (Sweden)

    Şükrü BAŞTÜRK

    2009-09-01

    Full Text Available The history of the studies on understanding, describing, introducing and analysing the literary text goes back to old times. Explanation of texts, the efforts of describing literary text and through facilitating in understanding the text can be extended from the period of Divan literature to nowadays. Today explanation of texts gains a lot of attention from the aspects of not only introducing and evaluating the past but also establishing a link between the past and the present. In the article, appropriate information about explanation, preamble and also “Gülistan” by Sadi and “Gülistan Dibace” are given and besides Rüşdî and his “Şerh-i Dibâce-i Gülistan” are introduced. Furthermore by giving Turkish words that belonged to Turkish Language of the XVI. Century in Dibace and could reach or not reach to the present time, it was aimed to provide contribution to the vocabulary existence of Turkish Language. Edebî metinleri anlama, açıklama, tanıtma ve değerlendirme amacıyla yapılan çalışmaların tarihi eskilere uzanmaktadır. Metin şerhi, edebî metinleri açıklama ve böylece metnin anlaşılmasını kolaylaştırma çabaları, Divan edebiyatı döneminden günümüze kadar gelmiştir. Günümüzde ise metin şerhi geçmişin tanıtılması ve değerlendirilmesi, geçmişle bugün arasında bağlantı kurulması açısından daha da önem kazanmış bulunmaktadır.Bu makalede şerh, dibace, Sadi'nin Gülistan'ı ve Gülistan Dibacesi şerhleri hakkında bilgi verilip Rüşdî ve Şerh-i Dibâce-i Gülistan'ı tanıtılmış, ayrıca Dibace'deki XVI. yüzyıl Türkçesine ait günümüz yazı diline ulaşan ve ulaşmayan Türkçe kelimeler verilerek Türkçenin söz varlığına katkı sağlamak amaçlanmıştır.

  12. 图书馆标准体系构建的理论依据、参照指标与建设基础%A Study on Theoretical Basis, Reference Points of Ideas and Foundation of Stan-dards for Libraries

    Institute of Scientific and Technical Information of China (English)

    周文杰

    2016-01-01

    This study conducted a systematic analysis of the constructing of the standard and norm system of library through perspectives of theoretical basis, reference points of ideas and foundations. Form the perspective of theoretical basis, Three Worlds Theory which developed by Karl R. Popper explained the possibilities, Five Laws of Library Sci-ence which issued by S. R. Ranganathan explained the necessity, and social epistemology explained the feasibility of the standard system of library. Besides, ideas from the library related standard which conducted by ISO and IFLA and some quality evaluation program provided reference points for library standard in China. Social foundation, professional foun-dation, policy foundation and methodology foundation should be the essential foundation of the construction of library standard and norms in China.%文章从理论依据、参照标准和建设基础三个方面对我国图书馆标准体系构建展开了分析。就图书馆标准体系的理论依据而言,“世界三”理论解释了其可能性,“图书馆学五定律”解释了其必要性,而“社会认识论”解释了其现实性。ISO及IFLA所制定的与图书馆相关的标准及国际图书馆界开展的若干质量评估项目及其体现的理念为构建我国图书馆标准体系提供了参照。我国图书馆标准体系的构建已具备了社会、职业、政策和方法论的基础。

  13. THE VISIT OF PANAGIS TSALDARIS, THE PRIME MINISTER OF GREECE, TO TURKEY (10-17 SEPTEMBER 1933 / YUNANİSTAN BAŞBAKANI PANAGİS TSALDARİS’İN TÜRKİYE’Yİ ZİYARETİ (10–17 EYLÜL 1933

    Directory of Open Access Journals (Sweden)

    Dr. Zafer ÇAKMAK

    2007-11-01

    Full Text Available With the effect of revisionist policies of Bulgaria in Balkans, a peace treaty was decided to be signed between Greece and Turkey. Panagis Tsaldaris, the Prime Minister of Greece, made an official visit to Turkey in 10-17 September 1933, both to sign this treaty and improve the relations between the two countries. The Prime Minister of Greece came to Istanbul in September 10th 1933, with Helli Cruiser of Greece Navy. Travelling from Istanbul to Ankara in September 11th by a special train prepared just for himself, he was greeted by Prime Minister Ismet (Inonu Pasha with official ceremony. Panagis Tsaldaris was accepted by President Gazi Mustafa Kemal (Ataturk in the first day of his visit in Ankara. He attended the ceremony of signing the treaty between Turkey and Greece that envisages friendship and cooperation in 14th of September. After the signing of this treaty, Panagis Tsaldaris visited some institutes and then left Ankara for Istanbul in 15th of September. After completing some visits and observations, Greece’s Prime Minister left Turkey in 17th of September.

  14. VIDAS(R)实现致病菌的全自动检测——访生物梅里埃公司行业科学事务总监Stan Bailey%VIDAS can Detect the Pathogen Automatically

    Institute of Scientific and Technical Information of China (English)

    卜庆婧

    2008-01-01

    @@ 据统计,我国每年发生的由致病菌引起的食物中毒事件占食物中毒事件总数的30%-90%,中毒人数占食物中毒总人数的60%-90%.因此,致病菌的检测应引起我们足够的重视.

  15. 浅析高中思想政治新课程标准对说课的新要求%A Brief Analysis on the New Requirements for Lesson Plan Presentation Proposed by the New Curriculum Stan-dard for Senior High School Ideological and Political Course

    Institute of Scientific and Technical Information of China (English)

    邹贵波

    2014-01-01

    Lesson plan presentation is a newly sprouted teaching and research form in China's recent years of teaching reform. Starting from the differences between the new and old curriculum standards for senior high school ideological and political course, this paper explores the new requirements, goals and methods of the lesson plan presentation for ideological and political course under the new curriculum concept, so as to further develop and improve lesson plan presentation guided by the new curriculum standard, and realize the organic combination of the new curricu-lum standard for senior high school ideological and political course and the practice of lesson plan presentation.%说课这种教研形式是我国这些年来教学改革中涌现出来的新生事物。本文从高中思想政治新旧课程标准的区别入手,探讨了思想政治说课在新课程理念之下的新要求、新目标和新方法,从而使说课活动在新的课程标准指导下进一步发展完善,实现高中思想政治新课程标准和说课实践的有机结合。

  16. The influence of operating loads on the state of stress and strain in selected load-bearing elements of a tower-type headgear structure / Wpływ obciążeń eksploatacyjnych na stan naprężenia oraz przemieszczenia wybranych elementów nośnych konstrukcji basztowej wieży szybowych

    Science.gov (United States)

    Wolny, Stanisław

    2012-12-01

    The headgear structure allows the conveyance to be moved over the shaft top to the loading (unloading) point, at the same time it keeps in place the rope pulleys while tower-type headgear structures also accommodate the entire winder installations. The headgear is where the final stage of the hoisting installation is located and where the surface transport systems begin. These aspects strongly impact the actual shape of the tower, its height and in some cases determine the design of the entire winding gear. In order that all the headgear functions should be provided, it is required that the ultimate state conditions should be maintained throughout its entire service life. In order to assess the critical service conditions, the computation procedure should be applied based on design loads and fatigue endurance parameters. The computations of characteristic loads acting on the headgear structure use the developed model of the system based on the dynamic analysis carried out for a specific case: a hoisting installation operated in one of the underground collieries in Poland. The maximal and minimal loads acting on a Koepe pulley and those required for the system operation are determined accordingly. The laws of dynamics provide a background for finding the forces and moments of forces acting in the components of the driving system (including the electric motors and pulley blocks) for the specified loading of the Koepe pulley. Underlying the numerical FEM model of the tower-type headgear structure are the technical specifications of the analysed object and FEM calculations followed by endurance analysis to find the state of stress in structural elements of the headgear under the typical service conditions. The results help in assessing how the design of the hoisting installation should impact on safety features of load-bearing elements in the headgear structure.

  17. Repressor SmeT of Efflux Pump SmeDEF in Stenotrophomonas maltophilia%嗜麦芽寡养单胞菌临床株多重耐药外排泵SmeDEF调控基因smeT的序列分析

    Institute of Scientific and Technical Information of China (English)

    孙二琳; 宋诗铎; 祁伟; 王玉宝

    2006-01-01

    目的了解嗜麦芽寡养单胞菌的临床株中,SmeDEF外排泵表达调控基因smeT与耐药性有关的序列变化.方法对嗜麦芽寡养单胞菌smeD-smeT基因间区和部分smeT序列进行PCR扩增,并对扩增产物进行序列分析;提取的RNA并加聚A尾,对smeT的3'端进行分析.结果选取耐药、敏感的泵抑制阳性株和耐药、敏感的泵抑制阴性嗜麦芽寡养单胞菌7株;发现对氟喹诺酮类耐药的泵抑制阳性菌的smeD-smeT间区测序基因序列与敏感株明显不同;所检测7株菌无论敏感或耐药在SmeT序列的166位氨基酸均为Leu,泵抑制阳性耐药株的SmeT序列在218位氨基酸为Asp/Glu替换.结论嗜麦芽寡养单胞菌临床株在相应于smeT的-165~-82区序列差异,以及SmeT的Asp218Glu氨基酸替换和多个位点的突变可能与多重外排的耐药有关.

  18. Efifcacy of bilateral anterior cervical­shoulder skin lfaps in the treatment of cicatricial contracture def­ ormity of neck%双侧颈肩皮瓣修复颈前瘢痕挛缩畸形疗效探讨

    Institute of Scientific and Technical Information of China (English)

    王运成; 翟刚; 吴永芳

    2014-01-01

    目的:探讨双侧颈肩皮瓣修复颈前瘢痕挛缩畸形的方法及疗效。方法选取2008年本院烧伤整形科收治的22例烧伤后颈前瘢痕挛缩畸形患者为研究对象,首先切除患者颈前瘢痕,松解挛缩带,然后从侧颈向肩峰方向设计、形成双侧颈肩皮瓣,最后向内旋转交叉修复颈前创面。结果22例患者采用的44个皮瓣全部成活,其中,优18例(81.82%),良4例(18.18%),优良率达100.00%。患者颈部活动功能恢复良好,皮肤质软,外观及功能良好,供皮区瘢痕增生不明显,无一例继发性颈部瘢痕挛缩畸形。无一例患者要求二期修整术。22例患者获随访1~5年,随访率达100.00%,与治疗前相比,患者颈部活动度各项指标均有不同程度的改善,差异均具有显著性(t=41.7592、21.2191、30.6134、16.7351、27.3986、54.2873,P均<0.01)。同时,经治疗后患者的颈颏角显著改善,差异具有显著性(t=62.2417,P<0.01)。患者对治疗的满意度较高,且疗效显著。结论双侧颈肩皮瓣血运可靠,能充分覆盖修复颈前部大创面,是修复颈前瘢痕的一种可行方法。%ObjectiveTo evaluate the efifcacy of bilateral anterior cervical-shoulder skin lfaps for repairing cicatricial contracture deformity of neck and to improve the method for the repair of soft tissue defect of neck. Method22 cases with cicatricial contracture deformity of neck in Baise City People’s Hospital were repaired by ifrst of all removed cervical scar removal, released contracture, then the second forming a bilateral anterior cervical-shoulder skin lfaps along the lateral neck to the acromion, the last ifnally repaired the front wound of neck by inward rotation cross.Result22 cases with cicatricial contracture deformity of neck by burn were operated with this method, with excellent in 18 cases (81.82%), good in 4 cases (18.18%), and optimal rate of 100.00%. All 44 skin lfaps survived in the ifrst intention and both the function and conifguration were satisfactory without swelling and contracture. The upper limb function and cervical part activity of patients were as well inproved after one to ifve-year follow-up. There were signiifcant differerce in neck range of motion (t=41.7592, 21.2191, 30.6134, 16.7351, 27.3986, 54.2873,Pal<0.01), and mental cervical angle (t=62.2417, P<0.01) before and after treatment. There were high levels of satisfaction.ConclusionBilateral anterior cervical-shoulder skin lfaps is an ideal method for repairing the cicatricial contracture deformity of neck, with abundant blood supply and simple operative procedure. Thus it is worthy of clinical application.

  19. 食品安全国家标准发酵乳

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ 前言 本标准对应于国际食品法典委员会(CAC)的标准Codex Stan 243-2003(Revision 2008)CodexStandard for Fermented Milks,本标准与Codex Stan243-2003(Revision 2008)的一致性程度为非等效.

  20. NCBI nr-aa BLAST: CBRC-DMEL-02-0026 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DMEL-02-0026 ref|NP_724962.2| starry night CG11895-PA [Drosophila melanogaster...] sp|Q9V5N8|STAN_DROME Protocadherin-like wing polarity protein stan precursor (Protein starry night) (Prote

  1. NCBI nr-aa BLAST: CBRC-DYAK-02-0012 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DYAK-02-0012 ref|NP_724962.2| starry night CG11895-PA [Drosophila melanogaster...] sp|Q9V5N8|STAN_DROME Protocadherin-like wing polarity protein stan precursor (Protein starry night) (Prote

  2. NCBI nr-aa BLAST: CBRC-DSIM-02-0024 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-02-0024 ref|NP_724962.2| starry night CG11895-PA [Drosophila melanogaster...] sp|Q9V5N8|STAN_DROME Protocadherin-like wing polarity protein stan precursor (Protein starry night) (Prote

  3. NCBI nr-aa BLAST: CBRC-AGAM-01-0005 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-01-0005 ref|NP_724962.2| starry night CG11895-PA [Drosophila melanogaster...] sp|Q9V5N8|STAN_DROME Protocadherin-like wing polarity protein stan precursor (Protein starry night) (Prote

  4. 六月至潮鞋款

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    ①Adidas Skateboarding Stan Smith Adidas Originals将Superstar和Stan Smith两大经典系列延伸发展出适合滑板运动的实用板鞋。近期在Adidas网站上公布了几款Stan Smith板鞋,在之前我们曾经向大家展示了Stan Smith全黑蛇皮版本,本次,将为大家推荐这款白、绿配色的Stan Smith,干净的鞋身正适合在夏季穿着。由Krooked公司的设计师,之前也是著名滑手的Tommy Guerreri设计,值得期待!

  5. AcEST: BP916032 [AcEST

    Lifescience Database Archive (English)

    Full Text Available sp|Q642T7|DEF_XENTR Digestive organ expansion factor homolog OS=... 127 2e-29 sp|...Q5ZLG3|DEF_CHICK Digestive organ expansion factor homolog OS=... 126 3e-29 sp|Q68CQ4|DEF_HUMAN Digestive organ expansion... factor homolog OS=... 125 6e-29 sp|Q5M9G7|DEF_RAT Digestive organ expansion factor homolog OS=R...a... 125 1e-28 sp|Q8BTT6|DEF_MOUSE Digestive organ expansion factor homolog OS=...... 124 2e-28 sp|Q6PEH4|DEF_DANRE Digestive organ expansion factor OS=Danio re... 123 3e-28 sp|P40498|YIJ1_YEA

  6. Characterization of a defensin from the oyster Crassostrea gigas - Recombinant production, folding, solution structure, antimicrobial activities, and gene expression

    OpenAIRE

    Gueguen, Yannick; Herpin, Amaury; Aumelas, André; Garnier, Julien; Fievet, Julie; Escoubas, Jean-Michel; Bulet, Philippe; Gonzalez, Marcelo; Lelong, Christophe; Favrel, Pascal; Bachere, Evelyne

    2006-01-01

    In invertebrates, defensins were found in arthropods and in the mussels. Here, we report for the first time the identification and characterization of a defensin (Cg-Def) from an oyster. Cg-def mRNA was isolated from Crassostrea gigas mantle using an expressed sequence tag approach. To gain insight into potential roles of Cg-Def in oyster immunity, we produced the recombinant peptide in Escherichia coli, characterized its antimicrobial activities, determined its solution structure by NMR spec...

  7. Characterization of a defensin from the oyster Crassostrea gigas. Recombinant production, folding, solution structure, antimicrobial activities, and gene expression.

    Science.gov (United States)

    Gueguen, Yannick; Herpin, Amaury; Aumelas, André; Garnier, Julien; Fievet, Julie; Escoubas, Jean-Michel; Bulet, Philippe; Gonzalez, Marcelo; Lelong, Christophe; Favrel, Pascal; Bachère, Evelyne

    2006-01-06

    In invertebrates, defensins were found in arthropods and in the mussels. Here, we report for the first time the identification and characterization of a defensin (Cg-Def) from an oyster. Cg-def mRNA was isolated from Crassostrea gigas mantle using an expressed sequence tag approach. To gain insight into potential roles of Cg-Def in oyster immunity, we produced the recombinant peptide in Escherichia coli, characterized its antimicrobial activities, determined its solution structure by NMR spectroscopy, and quantified its gene expression in vivo following bacterial challenge of oysters. Recombinant Cg-Def was active in vitro against Gram-positive bacteria but showed no or limited activities against Gram-negative bacteria and fungi. The activity of Cg-Def was retained in vitro at a salt concentration similar to that of seawater. The Cg-Def structure shares the so-called cystine-stabilized alpha-beta motif (CS-alphabeta) with arthropod defensins but is characterized by the presence of an additional disulfide bond, as previously observed in the mussel defensin (MGD-1). Nevertheless, despite a similar global fold, the Cg-Def and MGD-1 structures mainly differ by the size of their loops and by the presence of two aspartic residues in Cg-Def. Distribution of Cg-def mRNA in various oyster tissues revealed that Cg-def is mainly expressed in mantle edge where it was detected by mass spectrometry analyses. Furthermore, we observed that the Cg-def messenger concentration was unchanged after bacterial challenge. Our results suggest that Cg-def gene is continuously expressed in the mantle and would play a key role in oyster by providing a first line of defense against pathogen colonization.

  8. Pop / Tõnis Kahu

    Index Scriptorium Estoniae

    Kahu, Tõnis, 1962-

    2008-01-01

    Heliplaatidest: Chemical Brothers "Brotherhood", Plutonium 74 "Peittoalueen ulkopuolella", Disturbed "Indestructible", Thomas Feiner & Anywhen "The Opiates Revised", DefRage "Save Us from Religion", Kago "Mopskassi maja"

  9. Pop / Tõnis Kahu

    Index Scriptorium Estoniae

    Kahu, Tõnis, 1962-

    2008-01-01

    Heliplaatidest: Chemical Brothers "Brotherhood", Plutonium 74 "Peittoalueen ulkopuolella", Disturbed "Indestructible", Thomas Feiner & Anywhen "The Opiates Revised", DefRage "Save Us from Religion", Kago "Mopskassi maja"

  10. Pop / Tõnu Kaalep

    Index Scriptorium Estoniae

    Kaalep, Tõnu, 1966-

    2005-01-01

    Heliplaatidest: Olav Ehala "Armastatud laulud", Rein Rannap "Tantsib klaveril", Annie "Anniemal", Freeform Five "Strangest Things", Handsome Boy Modeling School "White People", Def Räädu "Planet Duckrabbit"

  11. The Influence of Operating Loads on the State of Stress and Strain in Selected Load-Bearing Elements of a Tower-Type Headgear Structure in the Light of the Experimental Data / Wpływ Obciążeń Eksploatacyjnych Na Stan Naprężenia Oraz Przemieszczenia Elementów Nośnych Konstrukcji Basztowej Wieży Szybowej W Świetle Przeprowadzonych Eksperymentów

    Science.gov (United States)

    Wolny, Stanisław

    2012-12-01

    In order that the ultimate state method should be applied to the strength analysis of the tower-type headgear structure, it is required that the design loads and endurance parameters be first established. For that purpose the characteristics of loads experienced by structural elements of the headgear structure are required (Wolny, 2012) as well as the numerical analysis of stresses and strains. Thus obtained results are verified through stress (strain) measurements taken in structural elements subjected to highest loads found on the basis of the stress map derived from numerical analysis, being the subject matter of the present study. Strain (stress) measurements are taken on the beams located at the floor level +65.00 m on which the winding machines are positioned (drive shaft bearings, stators in the electric motors), as shown schematically in Fig. 2. The strength analysis by numerical methods is restricted to those elements of the load bearing structure in the headgear at the level (+65.00) where the strain (stress) and measurements are taken and where loads are measured that give rise to the maximal strain changes (Wolny, 2012). Alongside the strain (stress) measurements in the load-bearing elements of the headgear structure, measurements are taken of horizontal displacements at selected points of the structure with the use of an interferometric radar IBIS-S. Results of the repeated numerical analysis of the state of stress, restricted to those elements of the load bearing structure in the tower-type headgear where the maximal loads are registered (Wolny, 2010), agree well with experimental data obtained from tests done on a real object. Therefore, the numerical analyses of the state of stress and strain in the load-bearing elements of the headgear structures operated in the Polish collieries lead us to the assumption that when analysing the geometry of the driving systems in the winding gear, the structures on which the elements of the winder installation are positioned ought to be treated as rigid. This conclusion is further corroborated by displacement measurements by geodetic methods taken on selected points of the tower-type headgear structure. An interesting point is that the tower-type headgear structure above its first floor level will behave as a rigid solid.

  12. Memories of a visiting scientist

    Energy Technology Data Exchange (ETDEWEB)

    Mytilineou, Eugenia [Physics Department, University of Patras, GR-26500 Patra (Greece)

    2012-10-15

    Stan Ovshinsky, the man who practically started the field of amorphous semiconductors in the West, is 90 years old this year. He always keeps the enthusiasm of a teenager and the slim and tall figure of a cypress tree. Stan's personality actually reminds me of these characteristic trees of the Mediterranean landscape; with their narrow tall figures pointing towards the sky and popping out of any green background surrounding them. Stan feels and behaves as a young man; even at the age of 85, following his old dream for changing the world, he founded a new company. I first met Stan and Iris in 1977. Since then, I have spent several sabbatical leaves in Energy Conversion Devices (ECD), from 1984 to 2004. In the following, our scientific collaboration and the build up of a very deep and sincere friendship will be described. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  13. EPA, USDA Join to Set Nations First Food Waste Reduction Goals with Charities and Private Companies

    Science.gov (United States)

    WASHINGTON -- On Wednesday, September 16, U.S. Environmental Protection Agency (EPA) Acting Deputy Administrator Stan Meiberg will join U.S. Agriculture Secretary Tom Vilsack as well as private industry and charitable organizations to announce the U

  14. Bogi i monstrõ, ili biografija Supermena / Olga Marsheva

    Index Scriptorium Estoniae

    Marsheva, Olga

    2007-01-01

    Ameerika tuntuimad koomiksikunstnikud Larry Hama, Stan Lee, Jerry Seagel, Bob Kain, Frank Miller ja nende loodud tegelaskujud. Ka briti kirjanik Alan Moore, kellele ei meeldi oma kangelastest tehtud ekraniseeringuid vaadata

  15. Roheline koletis / Laurence Lumiere

    Index Scriptorium Estoniae

    Lumiere, Laurence

    2008-01-01

    Koomiksipõhine seiklusfilm "Unustamatu Hulk" ("The Incredible Hulk") : stsenaristid Stan Lee ja Jack Kirby koomiksisarja alusel Zak Penn ning Edward Norton, kes kehastab ka peategelast : Ameerika Ühendriigid 2008. Lisatud nimekiri "20 edukamat koomiksifilmi Põhja-Ameerikas"

  16. Bogi i monstrõ, ili biografija Supermena / Olga Marsheva

    Index Scriptorium Estoniae

    Marsheva, Olga

    2007-01-01

    Ameerika tuntuimad koomiksikunstnikud Larry Hama, Stan Lee, Jerry Seagel, Bob Kain, Frank Miller ja nende loodud tegelaskujud. Ka briti kirjanik Alan Moore, kellele ei meeldi oma kangelastest tehtud ekraniseeringuid vaadata

  17. Üllatavalt mõjus "Uskumatu Hulk" / Alar Niineväli

    Index Scriptorium Estoniae

    Niineväli, Alar

    2008-01-01

    Koomiksipõhine seiklusfilm "Unustamatu Hulk" ("The Incredible Hulk") : stsenaristid Stan Lee ja Jack Kirby koomiksisarja alusel Zak Penn ning Edward Norton, kes kehastab ka peategelast : Ameerika Ühendriigid 2008

  18. an Unrecorded Alcohol Beverage

    African Journals Online (AJOL)

    NICO

    alcohol consumption, including malnutrition, selfneglect, poor hygiene, pale skin, weight .... Table 2 Analytical performance parameters of the GC-FID for the stan- dards (n = 3). .... Kluwer Academic Plenum Publishers,. New York, 1999, pp.

  19. Windmills and waterwheels explained what they do and how they work

    CERN Document Server

    Yorke, Stan

    2013-01-01

    Stan Yorke, using easy to follow text, diagrams and photographs, describes the various styles of mill and waterwheel and how they work. The book includes a list of restored mills which are open to the public.

  20. Using Rscript for Software Analysis

    NARCIS (Netherlands)

    Klint, P.

    2008-01-01

    RSCRIPT is a concept language that explores the design space of relation-based languages for software analysis. We briefly sketch the RSCRIPT language by way of a stan- dard example, summarize our experience, and point at fu- ture developments.

  1. Experiment list: SRX263903 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available stine Tissue MOLECULE=genomic DNA || DISEASE=iron deficiency, bipolar || BIOMATERIAL_PROVIDER=Shin Lin, Stan...LECTION_METHOD=Autopsy || DONOR_ID=STL002 || DONOR_AGE=30 || DONOR_HEALTH_STATUS=iron deficiency, bipolar

  2. Experiment list: SRX347281 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available stine Tissue MOLECULE=genomic DNA || DISEASE=iron deficiency, bipolar || BIOMATERIAL_PROVIDER=Shin Lin, Stan...LECTION_METHOD=Autopsy || DONOR_ID=STL002 || DONOR_AGE=30 || DONOR_HEALTH_STATUS=iron deficiency, bipolar

  3. EPA Holds National Sustainable Design Expo

    Science.gov (United States)

    WASHINGTON, DC - Environmental Protection Agency (EPA) Deputy Administrator Stan Meiburg will open the 11th Annual National Sustainable Design Expo Saturday morning in Alexandria, Va. The expo will host student teams from colleges and universities across t

  4. NJP VOLUME 40 No 4 Corrected

    African Journals Online (AJOL)

    PROF. EZECHUKWU

    2013-05-19

    May 19, 2013 ... laboratory data were obtained. The serum zinc ... administered to obtain the clinical data from the caregiver ... Systems, Hayward, California, USA).8 The Zn2+ Stan- ... Overall, the mean (SD) serum zinc level recorded in the.

  5. Cardiac function in offspring of women with diabetes using fetal ECG, umbilical cord blood pro-BNP, and neonatal interventricular septal thickness

    DEFF Research Database (Denmark)

    Halse, Karen; Lindegaard, Marie Louise Skakkebæk; Amer-Wahlin, Isis

    2013-01-01

    were included prospectively. Umbilical cord blood pro-BNP concentrations were measured immediately after delivery (n=68) and echocardiography was performed in the newborns (n=75). Fetal ECG in combination with cardiotocography, that is STAN technology was also performed during labor. Results......: The concentration of umbilical cord blood pro-BNP was associated positively with the neonatal cardiac interventricular septal thickness (P=0.025) and associated negatively with umbilical cord blood pH levels (P=0.036). Fetal ECG changes (STAN events) were recorded in 22 of 53 labors where STAN was used (42......%). No correlation was observed between interventricular septal thickness and STAN events. Conclusion: Increased umbilical cord blood pro-BNP is associated with echocardiographic signs of cardiomyopathy and with lower umbilical cord blood pH....

  6. 加强合作携手努力共同维护全球粮食安全在世界粮食安全峰会上的讲话%Strengthen Cooperation for Global Food Security Address at the World Food Summit

    Institute of Scientific and Technical Information of China (English)

    回良玉

    2010-01-01

    @@ Mr.Chair, Dear colleagues, Ladies and gentlemen, Let me stan by extending.on behalf of the Chinese Government,warm congratulations on the convocation of the World Food Summit and wishing the Summit acomplete success.

  7. Roheline koletis / Laurence Lumiere

    Index Scriptorium Estoniae

    Lumiere, Laurence

    2008-01-01

    Koomiksipõhine seiklusfilm "Unustamatu Hulk" ("The Incredible Hulk") : stsenaristid Stan Lee ja Jack Kirby koomiksisarja alusel Zak Penn ning Edward Norton, kes kehastab ka peategelast : Ameerika Ühendriigid 2008. Lisatud nimekiri "20 edukamat koomiksifilmi Põhja-Ameerikas"

  8. Üllatavalt mõjus "Uskumatu Hulk" / Alar Niineväli

    Index Scriptorium Estoniae

    Niineväli, Alar

    2008-01-01

    Koomiksipõhine seiklusfilm "Unustamatu Hulk" ("The Incredible Hulk") : stsenaristid Stan Lee ja Jack Kirby koomiksisarja alusel Zak Penn ning Edward Norton, kes kehastab ka peategelast : Ameerika Ühendriigid 2008

  9. Completing the One EPA Web Transformation (Email Message)

    Science.gov (United States)

    Deputy administrator Stan Meiburg urged other administrators to review their web transformation progress, and make sure they have requested extensions and planned to temporarily transfer content to the www3 server, before the September 30, 2015 deadline.

  10. Remote sensing of sea state by the Brewster's angle technique

    Digital Repository Service at National Institute of Oceanography (India)

    Sathe, P; AjoyKumar

    stream_size 13 stream_content_type text/plain stream_name Def_Sci_J_40_119.pdf.txt stream_source_info Def_Sci_J_40_119.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

  11. Respiratory and hemodynamic effects of diminished expiratory flow during artificial ventilation

    NARCIS (Netherlands)

    W. van Rooyen (Willem)

    1986-01-01

    textabstractThe supposition, that a diminished expiratory flow (DEF) during artificial ventilation will improve blood-gas exchange. especially in obstructive pulmonary disease and that DEF improves blood-gas exchange better than a comparable positive end-expiratory pressure (PEEP. producing the same

  12. Dicty_cDB: SLE320 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available _1( AY524011 |pid:none) Paulownia tomentosa deficiens (DEF... 33 6.1 protein upda...2 mRNA, p... 221 2e-56 AY530539_1( AY530539 |pid:none) Mimulus ringens deficiens (DEF) mR... 34 4.7 AY524011

  13. Dicty_cDB: SLH545 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available _1( AY524011 |pid:none) Paulownia tomentosa deficiens (DEF... 33 6.6 protein update 2009. 1.24 PSORT psg: 0....0539_1( AY530539 |pid:none) Mimulus ringens deficiens (DEF) mR... 34 5.0 AY524011

  14. Integrated Battlefield Effects Research for the National Training Center. Appendix H. Designs of Nuclear and Chemical Field Simulators for the National Training Center

    Science.gov (United States)

    1984-12-31

    require too much power for a pocket application of the dosimeter, unless4 batteries were frequently replaced. Aplicability of a D motor with its feedbaCK...Under Sec of Def for Policy ATTN: RAEV ATTN: Dir Plng & Requirements, M. Sheridan ATTN: SPSS ATTN: SPTD Under Secy of Def for R. a Engrg ATTN: STBE

  15. Marine Corps Lessons Learned System (MCLLS) User’s Guide

    Science.gov (United States)

    1993-08-20

    LNCH COMPLEX) CHILE COMUSFORCARIB BMD (BALL MSLE DEF) CHINA COMUSKOREA BMDC (BALL MSLE DEF CTR) CHOP (CHANGE OF OP CNTL) COMUSMARFOR BMEWS (BAL MSL...WARN) SSP (STRAT STRIKE PLAN) TM SIDA (SlOP INTG DATA BSE) STAFF FUNCTIONS TM (TECHNICAL MANUAL) SIDAC (SGL IN DAM AS CAp) STANAG TNF (THEATER NUC

  16. Effect of Exponential Fertilization on Biomass Allocation,Photosynthesis and Root Morphology of Padus maackii Seedlings%指数施肥对斑叶稠李苗木生物量分配、光合作用及根系形态的影响

    Institute of Scientific and Technical Information of China (English)

    郝龙飞; 王庆成; 刘婷岩; 许丽娟

    2014-01-01

    Biomass allocation,photosynthesis and root morphology of Padus maackii one-year-old bare-root seedlings under constant fertilization ( CF ) , exponential fertilization ( EF ) , doubled exponential fertilization ( DEF ) and no fertilization (CK) regimes were investigated. 1) By the end of the growing season,the root/shoot ratio of seedlings under EF treatment was maximum,and was 6. 7%,14. 3%,23. 1% greater than that with CK,CF and DEF treatment ( P DEF>CF>CK. Seedling height and collar diameter of EF treatment was 16. 6% and 28. 1% higher than those of CK,respectively ( PEF >CF >DEF. The number of first-order lateral root of EF was 19,and more than that with CK,CF,DEF treatment (P0. 05) greater than that with CK,CF and DEF treatment,respectively. 5) Compared with OF treatment, average length of first-order roots ( absorptive roots ) under EF and DEF treatment was 15 . 8%,16 . 7% higher ( P 0 . 05 ) ,respectively; Specific root length of first-order root under EF and DEF treatment was 13. 9%,14. 7% longer than that with CF treatment (P >0. 05),respectively. The fifth-order root of EF treatment had greater average length,average diameter and specific root length compared with CF treatment. Exponential fertilization regimes effectively promoted photosynthetic rate and improved root morphology, meanwhile, increased biomass accumulation and nutrient absorption in P. maackii seedlings.

  17. JPRS Report, Near East & South Asia, Pakistan

    Science.gov (United States)

    1991-08-09

    edibles, is very alert indeed in apprehending those who adulterate alcoholic beverages . Regarding the aid issue the U.S. administration ensured He...border belt. After cutting the poppy bud, opium juice is derived which is processed by the crudest to more sophisticated In Girdi Jungle brown heroin is...stan, Bangladesh, Nepal, India, Iraq, Iran, Kurdistan, stan. According to a report published by our immigra- Thailand , Sri Lanka, and Burma. Uganda

  18. 食品安全国家标准干酪

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ 前言 本标准对应于国际食品法典委员会(CAC)的标准Codex Stan 283-1978(Revision 1999,Amend-ment2006,2008)Codex General Standard forCheese,本标准与Codex Stan 283-1978(Revi-sion 1999,Amendment 2006,2008)的一致性程度为非等效.

  19. Mercy Killings in Combat: Ending the Suffering of Gravely Wounded Combatants -- A Brief History, Applicable Law, Recent Prosecutions, and Proposals for Much Needed Change

    Science.gov (United States)

    2006-04-01

    the patient’s condition is thought to be an ’indignity.’ JOHN KEOWN , EUTHANASIA, ETHICS AND PUBLIC POLICY: AN ARGUMENT AGAINST LEGALISATION 10 (2002...Results (demonstrating the divergent opinions about the morality combat-related mercy killings). 514 See supra Part IV.A. 515 Captain Stan Martin ...would have been "much more likely to contest the case" on the grounds that he acted out of mercy. Telephone Interview with Captain Stan Martin , defense

  20. Plaadid / Villu Päärt

    Index Scriptorium Estoniae

    Päärt, Villu, 1972-

    2003-01-01

    Uutest plaatidest Sarah Connor "Unbelievable", Robbie Williams "Escapology", Stan Getz "The Definitive Stan Getz", Santana "Sunrise", Wallflowers "Red Letter Days", Robert Palmer "At His Very Best", INXS "Definitive", Hilary Hahn "Mendelssohn/Shostakovich", System Of A Down "Steal This Album!", Disturbed "Believe", "Divas Las Vegas", Yann Tiersen " C'Etait Ici", We Will Rock You " Original London Cast Recording", Busta Rhymes " It Ain't Safe No More", David Bowie "Best Of", Terence Trent D'Arby "Greatest Hits"

  1. Simulated data and code for analysis of herpetofauna response to forest management in the Missouri Ozarks.

    Science.gov (United States)

    Rota, Christopher T; Wolf, Alexander J; Renken, Rochelle B; Gitzen, Robert A; Fantz, Debby K; Montgomery, Robert A; Olson, Matthew G; Vangilder, Larry D; Millspaugh, Joshua J

    2016-12-01

    We present predictor variables and R and Stan code for simulating and analyzing counts of Missouri Ozark herpetofauna in response to three forest management strategies. Our code performs four primary purposes: import predictor variables from spreadsheets; simulate synthetic response variables based on imported predictor variables and user-supplied values for data-generating parameters; format synthetic data for export to Stan; and analyze synthetic data.

  2. Plaadid / Villu Päärt

    Index Scriptorium Estoniae

    Päärt, Villu, 1972-

    2003-01-01

    Uutest plaatidest Sarah Connor "Unbelievable", Robbie Williams "Escapology", Stan Getz "The Definitive Stan Getz", Santana "Sunrise", Wallflowers "Red Letter Days", Robert Palmer "At His Very Best", INXS "Definitive", Hilary Hahn "Mendelssohn/Shostakovich", System Of A Down "Steal This Album!", Disturbed "Believe", "Divas Las Vegas", Yann Tiersen " C'Etait Ici", We Will Rock You " Original London Cast Recording", Busta Rhymes " It Ain't Safe No More", David Bowie "Best Of", Terence Trent D'Arby "Greatest Hits"

  3. Simulated data and code for analysis of herpetofauna response to forest management in the Missouri Ozarks

    Directory of Open Access Journals (Sweden)

    Christopher T. Rota

    2016-12-01

    Full Text Available We present predictor variables and R and Stan code for simulating and analyzing counts of Missouri Ozark herpetofauna in response to three forest management strategies. Our code performs four primary purposes: import predictor variables from spreadsheets; simulate synthetic response variables based on imported predictor variables and user-supplied values for data-generating parameters; format synthetic data for export to Stan; and analyze synthetic data.

  4. Propriedades psicométricas da Dutch Fatigue Scale e Dutch Exertion Fatigue Scale: versão brasileira Propiedades psicométricas de la Dutch Fatigue Scale y Dutch Exertion Fatigue Scale: versión Brasileña Psychometric properties of the Dutch Fatigue Scale and the Dutch Exertion Fatigue Scale: Brazilian version

    Directory of Open Access Journals (Sweden)

    Andrea Fini

    2010-04-01

    Full Text Available Fadiga é uma resposta humana em diversas situações agudas e crônicas e também na população geral. Este artigo relata estudo realizado para estimar confiabilidade e validade da Dutch Fatigue Scale (DUFS e da Dutch Exertion Fatigue Scale (DEFS adaptadas para o Brasil. Trezentos pacientes ambulatoriais com insuficiência cardíaca e 64 voluntários responderam a DUFS e a DEFS vertidas para o português. Análise fatorial sobre o conjunto de itens das duas escalas produziu solução com um fator para cada escala (variância explicada = 53,9% e boas estimativas de confiabilidade pelo alfa de Chronbach (DUFS=0,85 e DEFS=0,92 foram obtidas. Na amostra de pacientes, os escores nas escalas foram positivamente associados com a classe funcional da insuficiência cardíaca (DUFS e DEFS p=0,000, com os escores de depressão (DUFS r s=0,63; p=0,00 e DEFS r s=0,55; p=0,00 e com distúrbio de sono (DUFS e DEFS p=0,000. As versões brasileiras mostraram propriedades semelhantes as das escalas originais.Fatiga es una respuesta humana en muchas situaciones agudas e crónicas e también en la populación general. Este artículo relata estudio realizado para estimar la confiabilidad e validad de la Dutch Fatigue Scale (DUFS y de la Dutch Exertion Fatigue Scale (DEFS adaptadas para o Brasil. Trescientos pacientes con insuficiencia cardiaca y 64 voluntarios respondieran a la DUFS y DEFS vertidas para lo portugués. El análisis factorial de los ítems ha producido solución con un factor para cada escala (variancia explicada = 53,9% y buenas estimativas de confiabilidad por lo alfa de Chronbach (DUFS=0,85 e DEFS=0,92 fueron obtenidas. En la muestra de pacientes, las puntuaciones en las escalas fueron positivamente asociadas con la clase funcional de la insuficiencia cardiaca (DUFS y DEFS p=0,000, los escores de depresión (DUFS r s=0,63; p=0,00 y DEFS r s=0,55; p=0,00, y con las alteraciones del sueno (DUFS y DEFS p=0,000. Las versiones brasileras mostraran

  5. Solvothermal synthesis and structure of 3D frameworks of Nd(III) and Y(III) with thiophene-2,5-dicarboxylate and N,N‧-diethylformamide

    Science.gov (United States)

    Sharma, Swati; Yawer, Mohd; Kariem, Mukaddus; Sheikh, Haq Nawaz

    2016-08-01

    Two new 3D MOFs [Nd2(TDA)3(DEF)2(H2O)]n (1) and [Y4(TDA)6(DEF)4]n (2) [Thiophene-2,5-dicarboxylic acid (H2TDA) and N,N‧-diethylformamide (DEF)] were synthesized by solvothermal method. They were characterized by elemental analyses, infrared spectroscopy and single crystal X-ray diffraction studies. The two MOFs (1) and (2) belong to the monoclinic system with space group P21/n and C 2 respectively. Structural characterizations by single-crystal X-ray crystallography reveal that 1 and 2 adopt three-dimensional frameworks constructed by cross-linking of rod shaped infinite chain secondary building unit (SBU) by thiophene-2,5-dicarboxylates as linker. These frameworks feature rhomboidal channels, inside which coordinated DEF/H2O solvent molecules are located. DEF plays pivotal role in reaction and design of MOFs. Thermogravimetric analysis shows that both MOFs are thermally robust.

  6. Heterologous expression and solution structure of defensin from lentil Lens culinaris

    Energy Technology Data Exchange (ETDEWEB)

    Shenkarev, Zakhar O. [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow (Russian Federation); Gizatullina, Albina K. [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow (Russian Federation); Moscow Institute of Physics and Technology (State University), Department of Physicochemical Biology and Biotechnology, Institutskii per., 9, 141700 Dolgoprudny, Moscow Region (Russian Federation); Finkina, Ekaterina I.; Alekseeva, Ekaterina A.; Balandin, Sergey V.; Mineev, Konstantin S. [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow (Russian Federation); Arseniev, Alexander S. [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow (Russian Federation); Moscow Institute of Physics and Technology (State University), Department of Physicochemical Biology and Biotechnology, Institutskii per., 9, 141700 Dolgoprudny, Moscow Region (Russian Federation); Ovchinnikova, Tatiana V., E-mail: ovch@ibch.ru [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow (Russian Federation); Moscow Institute of Physics and Technology (State University), Department of Physicochemical Biology and Biotechnology, Institutskii per., 9, 141700 Dolgoprudny, Moscow Region (Russian Federation)

    2014-08-22

    Highlights: • Lentil defensin Lc-def and its {sup 15}N-labeled analog were overexpressed in E. coli. • Lc-def is active against fungi, but does not inhibit growth of G+ and G− bacteria. • Lc-def spatial structure involves triple-stranded β-sheet and α-helix (CSαβ motif). • Lc-def is able to bind to anionic lipid vesicles under low-salt conditions. • NMR data revealed significant μs–ms mobility in the loops 1 and 3 of Lc-def. - Abstract: A new defensin Lc-def, isolated from germinated seeds of the lentil Lens culinaris, has molecular mass 5440.4 Da and consists of 47 amino acid residues. Lc-def and its {sup 15}N-labeled analog were overexpressed in Escherichia coli. Antimicrobial activity of the recombinant protein was examined, and its spatial structure, dynamics, and interaction with lipid vesicles were studied by NMR spectroscopy. It was shown that Lc-def is active against fungi, but does not inhibit the growth of Gram-positive and Gram-negative bacteria. The peptide is monomeric in aqueous solution and contains one α-helix and triple-stranded β-sheet, which form cysteine-stabilized αβ motif (CSαβ) previously found in other plant defensins. The sterically neighboring loop1 and loop3 protrude from the defensin core and demonstrate significant mobility on the μs–ms timescale. Lc-def does not bind to the zwitterionic lipid (POPC) vesicles but interacts with the partially anionic (POPC/DOPG, 7:3) membranes under low-salt conditions. The Lc-def antifungal activity might be mediated through electrostatic interaction with anionic lipid components of fungal membranes.

  7. A reasonable criterion of reactivities at the defective region of single-walled carbon nanotubes

    Science.gov (United States)

    Jia, Gui-Xiao; Li, Jun-Qian; Chen, Lin-Gang; Li, Yi; Ding, Kai-Ning; Zhang, Yong-Fan

    Defect directional curvature KD-def was proposed as a reasonable criterion for the reactivities and adduct structures at the defective region of single-walled carbon nanotubes (SWCNTs). B3LYP/6-31G* calculations for the [2 + 1] and [1 + 1] additions of a series of 11-layer (n, n) (n = 4-8) and six-layer (10,0) SWCNTs with Stone-Wales defects showed that the KD-def or its mean KM-def was a good index to judge the adduct structures and the reactivities. Adducts of the [2 + 1] additions were divided into two types: one was the adduct with the C-X-C configuration and corresponding to the large KD-def and the large binding energy, and another was the adduct with the closed -3MR structure and corresponding to the small KD-def and the small binding energy. It must be pointed out that, besides mainly relying on the KD-def, the adduct structures and the reactivities of the [2 + 1] additions had been weakly affected by topologic structures. The calculated results for the [1 + 1] additions of the 11-layer (5,5) SWCNT with defect A revealed that the binding energies monotonously increased with the KM-def.

  8. McNamara, Clifford, and the Burdens of Vietnam, 1965-1969 (Secretaries of Defense Historical Series, Volume 6)

    Science.gov (United States)

    2011-01-01

    influential and longest serving, Colonel (later Lieutenant General) Robert E. Purs- ley , served under three secretaries from 1966 to August 1972. Military...67, FRUS 1964-68, 5:381 -83. 79. McNamara, In Retrospect, 269 (quote); SecDef DPM for Pres, 19 May 67, FRUS 1964-68, 5:423-38; Shap- ley , Promise and...92 IIIA 50-64 I1IB 31-49 IVA 21-30 IVB 16-20 1VC 10-15 V 1-9. 20. Memo SecDef for Pres, 2 Mar 66, memo SecDef for SvcSecs, 2 Mar 66: fldr

  9. 2013 Development Effectiveness Review

    OpenAIRE

    Asian Development Bank (ADB)

    2014-01-01

    The 2013 Development Effectiveness Review (DEfR) is the seventh annual performance review of the Asian Development Bank (ADB) and the first to apply the revised corporate results framework. The DEfR uses the 89 performance indicators of the revised framework to assess progress in implementing Strategy 2020, ADB’s long-term strategic framework. It evaluates ADB’s strengths and identifies areas where performance needs to be improved. The findings of the 2013 DEfR will inform actions to improve ...

  10. Discrimination of frequency variance for tonal sequences.

    Science.gov (United States)

    Byrne, Andrew J; Viemeister, Neal F; Stellmack, Mark A

    2014-12-01

    Real-world auditory stimuli are highly variable across occurrences and sources. The present study examined the sensitivity of human listeners to differences in global stimulus variability. In a two-interval, forced-choice task, variance discrimination was measured using sequences of five 100-ms tone pulses. The frequency of each pulse was sampled randomly from a distribution that was Gaussian in logarithmic frequency. In the non-signal interval, the sampled distribution had a variance of σSTAN (2), while in the signal interval, the variance of the sequence was σSIG (2) (with σSIG (2) >  σSTAN (2)). The listener's task was to choose the interval with the larger variance. To constrain possible decision strategies, the mean frequency of the sampling distribution of each interval was randomly chosen for each presentation. Psychometric functions were measured for various values of σSTAN (2). Although the performance was remarkably similar across listeners, overall performance was poorer than that of an ideal observer (IO) which perfectly compares interval variances. However, like the IO, Weber's Law behavior was observed, with a constant ratio of ( σSIG (2)- σSTAN (2)) to σSTAN (2) yielding similar performance. A model which degraded the IO with a frequency-resolution noise and a computational noise provided a reasonable fit to the real data.

  11. Esimene koolipäev tuleb muusikavärviline / Arian Levin ; interv. Kadri Ratt, Doris-Katre Truuver

    Index Scriptorium Estoniae

    Levin, Arian

    2007-01-01

    Küsimustele bändielust vastavad 1. sept. Rakvere spordihallis koolinoorte huvimessil esinevad muusikud Arian Levin Def Räädust, Martin Teichmann Steelstormist ja Märt ning Mart Metsjõe The Sampsonicsist

  12. Theoretical Chemistry Study of the Hydrogen-bonded Interaction between Acylamine and Chloromethane Compounds

    Institute of Scientific and Technical Information of China (English)

    GE Qing-Yu; WANG Hai-Jun; CHEN Jian-Hua

    2005-01-01

    The hydrogen-bonded interaction between acylamine and chloromethane was studied using theoretical calculation methods. Looking the interaction system as a hydrogen-bonded complex, the geometric optimization of the interaction system was performed with HF and B3LYP methods at 6-311++G** level. Stable structures of these complexes were obtained. Binding energies and some other physical chemistry parameters of them were computed and compared. According to the calculation results, it can be identified that DMA (DMF or DEF) can form stable complex with chloromethane by the hydrogen-bonded interaction between them. The stable orders of these hydrogen-bonded complexes were obtained and described as: DMF-CHCl3>DMF-CH2Cl2>DMF-CH3Cl, DEF-CHCl3>DEF-CH2Cl2>DEF-CH3Cl, DMA-CHCl3>DMA-CH2Cl2>DMA-CH3Cl, respectively.

  13. Popmuusika / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2005-01-01

    Uutest heliplaatidest Audio Bullys "Generation", Babyface "Grown & Sexy", "Def Jazz", Arch Enemy "Doomsday Machine", Four Tet "Everything Ecstatic", The BossHoss "Internashville", Rabih Abou Khalil "Journey To The Centre of an Egg"

  14. Popmuusika / Margus Haav

    Index Scriptorium Estoniae

    Haav, Margus, 1969-

    2006-01-01

    Uutest heliplaatidest Def Räädu "Succiety", Sepeltura "The BEst Of", "Reich Remixed", Chris Rea "The Road To Hell & Back", Solveig Slettahjell "Good Rain", The Fratellis "Costello Music", Siouxsie And The Banshees "VoicesOn The Air"

  15. Pop / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2006-01-01

    Heliplaatidest: Ludacris "Release Therapy", Dead Moon "Echoes of the Past", Jet "Shine On", Rassakas ja sõbrad "Album vms", Thom Yorke "Eraser", Disco Ensemble "First Aid Kit", Def Räädu "Succiety", The Stranglers "Suite XVI"

  16. Plaadid / Mart Normet

    Index Scriptorium Estoniae

    Normet, Mart, 1979-

    2006-01-01

    Uutest heliplaatidest Põhjamaade Hirm "Maailma südame põhjast", "F Com Essentials", Q-Continuum Presents "This Is The Marienhof", Def Leppard "Yrah!", Nouvelle Vague "Bande A'Part", Leningrad Cowboys "Zombies Paradise", Sao Paris "La"

  17. Effects of selected defoliants in combination with insecticides on sweetpotato whitefly (Hemiptera: Aleyrodidae) and its parasitoids in cotton

    Science.gov (United States)

    Effects of two defoliants, Def (S, S, Stributylphosphorotrithioate) and Dropp (thidiazuron) alone and in combination with two commonly used insecticides, a pyrethroid, Karate (lambda-cyhalothrin) and an organophosphate, Guthion (azinphosmethyl) on sweetpotato whitefly, Bemisia tabaci Gennadius Bioty...

  18. Effects of exercise on energy-regulating hormones and appetite in men and women

    National Research Council Canada - National Science Library

    Todd A. Hagobian; Carrie G. Sharoff; Brooke R. Stephens; George N. Wade; J. Enrique Silva; Stuart R. Chipkin; Barry Braun

    ...), and four bouts without energy added to induce energy deficit (DEF). Concentrations of acylated ghrelin, insulin, and leptin, as well as appetite ratings were measured in response to a meal after a no-exercise baseline and both exercise conditions...

  19. Disease: H01266 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H01266 Hypercarotenemia and vitamin A deficiency Hypercarotenemia and vitamin A def...e identified in a patient with hypercarotenemia and hypovitaminosis A. J Nutr 137:2346-50 (2007) ...

  20. Muusika : Plaadid kauplusest Lasering

    Index Scriptorium Estoniae

    2005-01-01

    Heliplaatidest: Def Räädu "Planet Duckrabbit", Sõpruse Puiestee "XX sajandi lapsed", Ines "15 magamata ööd", Metro Luminal "Reboot", Helin-Mari Arder "Su aknast näen", Leonard Cohen "Dear Heather"

  1. Esimene koolipäev tuleb muusikavärviline / Arian Levin ; interv. Kadri Ratt, Doris-Katre Truuver

    Index Scriptorium Estoniae

    Levin, Arian

    2007-01-01

    Küsimustele bändielust vastavad 1. sept. Rakvere spordihallis koolinoorte huvimessil esinevad muusikud Arian Levin Def Räädust, Martin Teichmann Steelstormist ja Märt ning Mart Metsjõe The Sampsonicsist

  2. Plaadid / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2005-01-01

    Uutest heliplaatidest Ashanti "Concerte Rose", My Chemical Romance "Three Cheers For Sweet Revenge", Bee Gees "Number Ones", Def Leppard "Best of...", Vanessa-Mae "Choregraphy", Tencu "O O O", Kuti, Femi "Africa Shrine"

  3. Popmuusika / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2005-01-01

    Uutest heliplaatidest Audio Bullys "Generation", Babyface "Grown & Sexy", "Def Jazz", Arch Enemy "Doomsday Machine", Four Tet "Everything Ecstatic", The BossHoss "Internashville", Rabih Abou Khalil "Journey To The Centre of an Egg"

  4. Ruminal silage degradability and productivity of forage and grain-type sorghum cultivars Degradabilidade ruminal das silagens e produtividade de cultivares de sorgo de tipo forrageiro e granífero

    Directory of Open Access Journals (Sweden)

    José Antônio Resende

    2003-01-01

    Full Text Available Forages for feeding dairy cows should conciliate the potential for high dry matter yield per unit area, maximizing animal stocking rate, with high nutritive value, which enables decreasing the amount of concentrate feedstuffs per milk unit produced. Eighteen sorghum cultivars were cultivated 0.70 m apart and with 12 plants per linear meter. Plants harvested at the milk-to-dough stage were ensiled in the laboratory. Eleven agronomic and chemical traits were evaluated. Silage samples were incubated in situ and the effective ruminal dry matter degradation (DEF was calculated assuming a fractional passage rate of -0.04 h-1. Dry matter yield was 13.0 ± 2.3 (mean ± S.D. t ha-1. The neutral detergent fiber content was 50.3 ± 3.8 and the DEF was 48.8 ± 2.8% (dry matter basis. The average sorghum DEF was 84% of the DEF of a composite sample of 60 corn hybrids simultaneously cultivated, ensiled and ruminally incubated. The 12 forage-type cultivars were taller, more productive, had higher fiber content and lower DEF than the 6 grain and dual purpose-type cultivars. The acid detergent fiber content had the greatest correlation with DEF (r= -0.64. The linear model correlating DEF with productivity was: DEF=54.694 - 0.4449 x t of dry matter ha-1 (r²=0.14. Although there are cultivars that show high productivity and digestibility, it seems to be difficult to conciliate the maximum nutritive value with the maximum productivity of the sorghum crop.Forrageiras para alimentação de vacas leiteiras devem conciliar a alta produção de matéria seca por área, maximizando a taxa de lotação animal, com o alto valor nutritivo, capaz de reduzir a necessidade de alimentos concentrados por litro de leite produzido. Dezoito cultivares de sorgo foram cultivados com espaçamento de 0,70 m e 12 plantas por metro linear. As plantas colhidas ao atingirem o estádio leitoso-pastoso foram ensiladas em laboratório. Onze características agronômicas e químicas foram

  5. Plaadid / Mart Normet

    Index Scriptorium Estoniae

    Normet, Mart, 1979-

    2006-01-01

    Uutest heliplaatidest Põhjamaade Hirm "Maailma südame põhjast", "F Com Essentials", Q-Continuum Presents "This Is The Marienhof", Def Leppard "Yrah!", Nouvelle Vague "Bande A'Part", Leningrad Cowboys "Zombies Paradise", Sao Paris "La"

  6. Muusika : Plaadid kauplusest Lasering

    Index Scriptorium Estoniae

    2005-01-01

    Heliplaatidest: Def Räädu "Planet Duckrabbit", Sõpruse Puiestee "XX sajandi lapsed", Ines "15 magamata ööd", Metro Luminal "Reboot", Helin-Mari Arder "Su aknast näen", Leonard Cohen "Dear Heather"

  7. Plaadid / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2005-01-01

    Uutest heliplaatidest Ashanti "Concerte Rose", My Chemical Romance "Three Cheers For Sweet Revenge", Bee Gees "Number Ones", Def Leppard "Best of...", Vanessa-Mae "Choregraphy", Tencu "O O O", Kuti, Femi "Africa Shrine"

  8. Pop / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2006-01-01

    Heliplaatidest: Ludacris "Release Therapy", Dead Moon "Echoes of the Past", Jet "Shine On", Rassakas ja sõbrad "Album vms", Thom Yorke "Eraser", Disco Ensemble "First Aid Kit", Def Räädu "Succiety", The Stranglers "Suite XVI"

  9. THE INFLUENCE OF OGHUZ TURKISH AND USING OF SYNONYM COMPOUND VERB THAT WAS CONSTRUCTED AUXILIARY VERBS KIL- AND ET- ON THE SAYF-I SARAYI’S GULISTAN BI’T-TURKI SEYF-İ SARAYİNİN GÜLİSTAN TERCÜMESİNDE AYNI İSİM UNSURLARININ KIL- VE ET- YARDIMCI FİİLLERİYLE KULLANIMINDAN OLUŞAN EŞ DEĞER BİRLEŞİK FİİLLER VE OĞUZCA ETKİSİ

    Directory of Open Access Journals (Sweden)

    Mustafa YILDIZ

    2010-01-01

    Full Text Available Mamluk Kipchak is name of written language which have been used by Kipchak Turks of ın Egypt. Many works have been written this written language with which roughly have been used from in the middle of XIII. century towards at the end of XV. century. One of the argued subject is Oghuz Turkish influence in the works have been written in Mamluk Kipchak. The language of works in this area was replaced with Oghuz Turkish towards the end of the XV. Century.In this article, dealt with 44 synonym verb structure which was formed with same nouns element and ḳıl-/et- auxiliary verbs on the Gülistan bi’t-Turki which is one of the major works of the Mamluk Area. Memluk Kıpçakçası, Mısır’da Kıpçak Türkleri tarafından kullanılmış olan yazı dilinin adıdır. Aşağı yukarı XIII. yüzyılın ortalarından XV. yüzyılın sonuna kadar kullanılmış olan bu yazı dili ile pek çok eser kaleme alınmıştır. Söz konusu Türkçe ile yazılmış olan eserlerin dilinde tartışılan konulardan biri Oğuz Türkçesi tesiridir. Bu sahadaki eserlerin dili XV. yüzyılın sonlarına doğru yerini bütünüyle Oğuz Türkçesine bırakmıştır. Bu yazıda Memluk sahasının temel eserlerinden birisi olan Gülistan Tercümesinin dilinde kıl- ve et- yardımcı fiillerinin aynı isim unsurlarıyla oluşturduğu 44 tane eş değer birleşik fiil yapısı üzerinde durduk.

  10. Big defensins, a diverse family of antimicrobial peptides that follows different patterns of expression in hemocytes of the oyster Crassostrea gigas.

    Directory of Open Access Journals (Sweden)

    Rafael D Rosa

    Full Text Available BACKGROUND: Big defensin is an antimicrobial peptide composed of a highly hydrophobic N-terminal region and a cationic C-terminal region containing six cysteine residues involved in three internal disulfide bridges. While big defensin sequences have been reported in various mollusk species, few studies have been devoted to their sequence diversity, gene organization and their expression in response to microbial infections. FINDINGS: Using the high-throughput Digital Gene Expression approach, we have identified in Crassostrea gigas oysters several sequences coding for big defensins induced in response to a Vibrio infection. We showed that the oyster big defensin family is composed of three members (named Cg-BigDef1, Cg-BigDef2 and Cg-BigDef3 that are encoded by distinct genomic sequences. All Cg-BigDefs contain a hydrophobic N-terminal domain and a cationic C-terminal domain that resembles vertebrate β-defensins. Both domains are encoded by separate exons. We found that big defensins form a group predominantly present in mollusks and closer to vertebrate defensins than to invertebrate and fungi CSαβ-containing defensins. Moreover, we showed that Cg-BigDefs are expressed in oyster hemocytes only and follow different patterns of gene expression. While Cg-BigDef3 is non-regulated, both Cg-BigDef1 and Cg-BigDef2 transcripts are strongly induced in response to bacterial challenge. Induction was dependent on pathogen associated molecular patterns but not damage-dependent. The inducibility of Cg-BigDef1 was confirmed by HPLC and mass spectrometry, since ions with a molecular mass compatible with mature Cg-BigDef1 (10.7 kDa were present in immune-challenged oysters only. From our biochemical data, native Cg-BigDef1 would result from the elimination of a prepropeptide sequence and the cyclization of the resulting N-terminal glutamine residue into a pyroglutamic acid. CONCLUSIONS: We provide here the first report showing that big defensins form a family

  11. Hot Deformation Behavior of As-Cast 2101 Grade Lean Duplex Stainless Steel and the Associated Changes in Microstructure and Crystallographic Texture

    Science.gov (United States)

    Patra, Sudipta; Ghosh, Abhijit; Singhal, Lokesh Kumar; Podder, Arijit Saha; Sood, Jagmohan; Kumar, Vinod; Chakrabarti, Debalay

    2016-11-01

    The hot deformation behavior of 2101 grade lean duplex stainless steel (DSS, containing 5 wt pct Mn, 0.2 wt pct N, and 1.4 wt pct Ni) and associated microstructural changes within δ-ferrite and austenite (γ) phases were investigated by hot-compression testing in a GLEEBLE 3500 simulator over a range of deformation temperatures, T def [1073 K to 1373 K (800 °C to 1100 °C)], and applied strains, ɛ (0.25 to 0.80), at a constant true strain rate of 1/s. The microstructural softening inside γ was dictated by discontinuous dynamic recrystallization (DDRX) at a higher T def [1273 K to 1373 K (1000 °C to 1100 °C)], while the same was dictated by continuous dynamic recrystallization (CDRX) at a lower T def (1173 K (900 °C)]. Dynamic recovery (DRV) and CDRX dominated the softening inside δ-ferrite at T def ≥ 1173 K (900 °C). The dynamic recrystallization (DRX) inside δ and γ could not take place upon deformation at 1073 K (800 °C). The average flow stress level increased 2 to 3 times as the T def dropped from 1273 to 1173 K (1000 °C to 900 °C) and finally to 1073 K (800 °C). The average microhardness values taken from δ-ferrite and γ regions of the deformed samples showed a different trend. At T def of 1373 K (1100 °C), microhardness decreased with the increase in strain, while at T def of 1173 K (900 °C), microhardness increased with the increase in strain. The microstructural changes and hardness variation within individual phases of hot-deformed samples are explained in view of the chemical composition of the steel and deformation parameters (T def and ɛ).

  12. Hot Deformation Behavior of As-Cast 2101 Grade Lean Duplex Stainless Steel and the Associated Changes in Microstructure and Crystallographic Texture

    Science.gov (United States)

    Patra, Sudipta; Ghosh, Abhijit; Singhal, Lokesh Kumar; Podder, Arijit Saha; Sood, Jagmohan; Kumar, Vinod; Chakrabarti, Debalay

    2017-01-01

    The hot deformation behavior of 2101 grade lean duplex stainless steel (DSS, containing 5 wt pct Mn, 0.2 wt pct N, and 1.4 wt pct Ni) and associated microstructural changes within δ-ferrite and austenite ( γ) phases were investigated by hot-compression testing in a GLEEBLE 3500 simulator over a range of deformation temperatures, T def [1073 K to 1373 K (800 °C to 1100 °C)], and applied strains, ɛ (0.25 to 0.80), at a constant true strain rate of 1/s. The microstructural softening inside γ was dictated by discontinuous dynamic recrystallization (DDRX) at a higher T def [1273 K to 1373 K (1000 °C to 1100 °C)], while the same was dictated by continuous dynamic recrystallization (CDRX) at a lower T def (1173 K (900 °C)]. Dynamic recovery (DRV) and CDRX dominated the softening inside δ-ferrite at T def ≥ 1173 K (900 °C). The dynamic recrystallization (DRX) inside δ and γ could not take place upon deformation at 1073 K (800 °C). The average flow stress level increased 2 to 3 times as the T def dropped from 1273 to 1173 K (1000 °C to 900 °C) and finally to 1073 K (800 °C). The average microhardness values taken from δ-ferrite and γ regions of the deformed samples showed a different trend. At T def of 1373 K (1100 °C), microhardness decreased with the increase in strain, while at T def of 1173 K (900 °C), microhardness increased with the increase in strain. The microstructural changes and hardness variation within individual phases of hot-deformed samples are explained in view of the chemical composition of the steel and deformation parameters ( T def and ɛ).

  13. The role of iron in Libby amphibole-induced acute lung injury and inflammation.

    Science.gov (United States)

    Shannahan, Jonathan H; Ghio, Andrew J; Schladweiler, Mette C; McGee, John K; Richards, Judy H; Gavett, Stephen H; Kodavanti, Urmila P

    2011-05-01

    Complexation of host iron (Fe) on the surface of inhaled asbestos fibers has been postulated to cause oxidative stress contributing to in vivo pulmonary injury and inflammation. We examined the role of Fe in Libby amphibole (LA; mean length 4.99 µm ± 4.53 and width 0.28 µm ± 0.19) asbestos-induced inflammogenic effects in vitro and in vivo. LA contained acid-leachable Fe and silicon. In a cell-free media containing FeCl(3), LA bound #17 µg of Fe/mg of fiber and increased reactive oxygen species generation #3.5 fold, which was reduced by deferoxamine (DEF) treatment. In BEAS-2B cells exposure to LA, LA loaded with Fe (FeLA), or LA with DEF did not increase HO-1 or ferritin mRNA expression. LA increased IL-8 expression, which was reduced by Fe loading but increased by DEF. To determine the role of Fe in LA-induced lung injury in vivo, spontaneously hypertensive rats were exposed intratracheally to either saline (300 µL), DEF (1 mg), FeCl(3) (21 µg), LA (0.5 mg), FeLA (0.5 mg), or LA + DEF (0.5 mg). LA caused BALF neutrophils to increase 24 h post-exposure. Loading of Fe on LA but not chelation slightly decreased neutrophilic influx (LA + DEF > LA > FeLA). At 4 h post-exposure, LA-induced lung expression of MIP-2 was reduced in rats exposed to FeLA but increased by LA + DEF (LA + DEF > LA > FeLA). Ferritin mRNA was elevated in rats exposed to FeLA compared to LA. In conclusion, the acute inflammatory response to respirable fibers and particles may be inhibited in the presence of surface-complexed or cellular bioavailable Fe. Cell and tissue Fe-overload conditions may influence the pulmonary injury and inflammation caused by fibers.

  14. Reduced Order Modeling Methods for Turbomachinery Design

    Science.gov (United States)

    2009-03-01

    be discussed in Subsection 1.2.3, which assume normally distributed response. Results also showed sig- nif cant mistuning located at the frequency of...conventionally def ned with Probability Density Functions ( PDF ) and the output are response statistics and PDF . It is an alter- native to deterministic analysis...certainty typically refers to def ning PDF for input parameters, but there are also non- probability based methods for quantif cation such as Bayesian [45

  15. Optimisation of transgene action at the post-transcriptional level: high quality parthenocarpic fruits in industrial tomatoes

    Directory of Open Access Journals (Sweden)

    Defez Roberto

    2002-01-01

    Full Text Available Abstract Background Genetic engineering of parthenocarpy confers to horticultural plants the ability to produce fruits under environmental conditions that curtail fruit productivity and quality. The DefH9-iaaM transgene, whose predicted action is to confer auxin synthesis specifically in the placenta, ovules and derived tissues, has been shown to confer parthenocarpy to several plant species (tobacco, eggplant, tomato and varieties. Results UC82 tomato plants, a typical cultivar used by the processing industry, transgenic for the DefH9-iaaM gene produce parthenocarpic fruits that are malformed. UC82 plants transgenic for the DefH9-RI-iaaM, a DefH9-iaaM derivative gene modified in its 5'ULR by replacing 53 nucleotides immediately upstream of the AUG initiation codon with an 87 nucleotides-long sequence derived from the rolA intron sequence, produce parthenocarpic fruits of high quality. In an in vitro translation system, the iaaM mRNA, modified in its 5'ULR is translated 3–4 times less efficiently than the original transcript. An optimal expressivity of parthenocarpy correlates with a reduced transgene mRNA steady state level in DefH9-RI-iaaM flower buds in comparison to DefH9-iaaM flower buds. Consistent with the known function of the iaaM gene, flower buds transgenic for the DefH9-RI-iaaM gene contain ten times more IAA than control untransformed flower buds, but five times less than DefH9-iaaM flower buds. Conclusions By using an auxin biosynthesis transgene downregulated at the post-transcriptional level, an optimal expressivity of parthenocarpy has been achieved in a genetic background not suitable for the original transgene. Thus, the method allows the generation of a wider range of expressivity of the desired trait in transgenic plants.

  16. Influence of photon beam energy on the dose enhancement factor caused by gold and silver nanoparticles: An experimental approach

    Energy Technology Data Exchange (ETDEWEB)

    Guidelli, Eder José, E-mail: ederguidelli@pg.ffclrp.usp.br; Baffa, Oswaldo [Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, 14040-901 Ribeirão Preto, SP (Brazil)

    2014-03-15

    Purpose: Noble metal nanoparticles have found several medical applications in the areas of radiation detection; x-ray contrast agents and cancer radiation therapy. Based on computational methods, many papers have reported the nanoparticle effect on the dose deposition in the surrounding medium. Here the authors report experimental results on how silver and gold nanoparticles affect the dose deposition in alanine dosimeters containing several concentrations of silver and gold nanoparticles, for five different beam energies, using electron spin resonance spectroscopy (ESR). Methods: The authors produced alanine dosimeters containing several mass percentage of silver and gold nanoparticles. Nanoparticle sizes were measured by dynamic light scattering and by transmission electron microscopy. The authors determined the dose enhancement factor (DEF) theoretically, using a widely accepted method, and experimentally, using ESR spectroscopy. Results: The DEF is governed by nanoparticle concentration, size, and position in the alanine matrix. Samples containing gold nanoparticles afford a DEF higher than 1.0, because gold nanoparticle size is homogeneous for all gold concentrations utilized. For samples containing silver particles, the silver mass percentage governs the nanoparticles size, which, in turns, modifies nanoparticle position in the alanine dosimeters. In this sense, DEF decreases for dosimeters containing large and segregated particles. The influence of nanoparticle size-position is more noticeable for dosimeters irradiated with higher beam energies, and dosimeters containing large and segregated particles become less sensitive than pure alanine (DEF < 1). Conclusions: ESR dosimetry gives the DEF in a medium containing metal nanoparticles, although particle concentration, size, and position are closely related in the system. Because this is also the case as in many real systems of materials containing inorganic nanoparticles, ESR is a valuable tool for

  17. Two separate molecular systems, Dachsous/Fat and Starry night/Frizzled, act independently to confer planar cell polarity.

    Science.gov (United States)

    Casal, José; Lawrence, Peter A; Struhl, Gary

    2006-11-01

    Planar polarity is a fundamental property of epithelia in animals and plants. In Drosophila it depends on at least two sets of genes: one set, the Ds system, encodes the cadherins Dachsous (Ds) and Fat (Ft), as well as the Golgi protein Four-jointed. The other set, the Stan system, encodes Starry night (Stan or Flamingo) and Frizzled. The prevailing view is that the Ds system acts via the Stan system to orient cells. However, using the Drosophila abdomen, we find instead that the two systems operate independently: each confers and propagates polarity, and can do so in the absence of the other. We ask how the Ds system acts; we find that either Ds or Ft is required in cells that send information and we show that both Ds and Ft are required in the responding cells. We consider how polarity may be propagated by Ds-Ft heterodimers acting as bridges between cells.

  18. Egg drop syndrome virus enters duck embryonic fibroblast cells via clathrin-mediated endocytosis.

    Science.gov (United States)

    Huang, Jingjing; Tan, Dan; Wang, Yang; Liu, Caihong; Xu, Jiamin; Wang, Jingyu

    2015-12-02

    Previous studies of egg drop syndrome virus (EDSV) is restricted to serological surveys, disease diagnostics, and complete viral genome analysis. Consequently, the infection characteristics and entry routes of EDSV are poorly understood. Therefore, we aimed to explore the entry pathway of EDSV into duck embryonic fibroblast (DEF) cells as well as the infection characteristics and proliferation of EDSV in primary DEF and primary chicken embryo liver (CEL) cells. Transmission electron microscopy revealed that the virus triggered DEF cell membrane invagination as early as 10 min post-infection and that integrated endocytic vesicles formed at 20 min post-infection. The virus yield in EDSV-infected DEF cells treated with chlorpromazine (CPZ), sucrose, methyl-β-cyclodextrin (MβCD), or NH4Cl was measured by quantitative real-time PCR. Compared with the mock treatment, CPZ and sucrose greatly inhibited the production of viral progeny in a dose-dependent manner, while MβCD treatment did not result in a significant difference. Furthermore, NH4Cl had a strong inhibitory effect on the production of EDSV progeny. In addition, indirect immunofluorescence demonstrated that virus particles clustered on the surface of DEF cells treated with CPZ or sucrose. These results indicate that EDSV enters DEF cells through clathrin-mediated endocytosis followed by a pH-dependent step, which is similar to the mechanism of entry of human adenovirus types 2 and 5. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Functional characterization of two defensin isoforms of the hard tick Ixodes ricinus

    Directory of Open Access Journals (Sweden)

    Růžek Daniel

    2011-04-01

    Full Text Available Abstract Background The immune system of ticks is stimulated to produce many pharmacologically active molecules during feeding and especially during pathogen invasion. The family of cationic peptides - defensins - represents a specific group of antimicrobial compounds with six conserved cysteine residues in a molecule. Results Two isoforms of the defensin gene (def1 and def2 were identified in the European tick Ixodes ricinus. Expression of both genes was induced in different tick organs by a blood feeding or pathogen injection. We have tested the ability of synthetic peptides def1 and def2 to inhibit the growth or directly kill several pathogens. The antimicrobial activities (expressed as minimal inhibition concentration and minimal bactericidal concentration values against Gram positive bacteria were confirmed, while Gram negative bacteria, yeast, Tick Borne Encephalitis and West Nile Viruses were shown to be insensitive. In addition to antimicrobial activities, the hemolysis effect of def1 and def2 on human erythrocytes was also established. Conclusions Although there is nothing known about the realistic concentration of defensins in I. ricinus tick body, these results suggest that defensins play an important role in defence against different pathogens. Moreover this is a first report of a one amino acid substitution in a defensins molecule and its impact on antimicrobial activity.

  20. CCDC 972510: Experimental Crystal Structure Determination : 8,21-bis(4-t-butylphenyl)-2,15-bis(2,6-diisopropylphenyl)-3a,5a,7,13a,16a,16b,20a,20b,22d,24a,26a,26b-dodecahydrobenzo[1,12]benzo[13',14']isoquinolino[6'',5'',4'':8',9',10']pentapheno[2',3':3,4]tetrapheno[9,8,7-def]isoquinoline-1,3,14,16(2H,15H)-tetrone toluene acetonitrile solvate

    KAUST Repository

    Das, Soumyajit

    2014-01-01

    An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

  1. An improved TERCOM algorithm and its application in a submarine terrain aided navigation system

    Science.gov (United States)

    Xu, Zunyi; Yan, Lei; Liu, Guangjun; Wang, Hua

    2006-10-01

    Terrain contour matching (TERCOM) algorithm is the main one in the conventional terrain aided navigation system. When it has been directly applied to the submarine terrain aided navigation (STAN) system, the accuracy and stability are primely reduced. To solve these problems, the detail analyses about the particularities of the STAN and the disadvantages of the conventional TERCOM algorithm have been made. The improvement for the TERCOM algorithm on the measurement function, the water depth survey error and the scheme to prevent fail positioning has been done. Moreover, experiments with pedestrian simulation confirm the effectiveness of the improved TERCOM algorithm. The system achieves higher positioning precision than that of conventional one.

  2. Test pilots 1962 - Armstrong, Walker, Dana, Peterson, McKay, Thompson, Butchart

    Science.gov (United States)

    1962-01-01

    The research pilots at what in 1962 was called the Flight Research Center standing in front of the X-1E. They are (left to right) Neil Armstrong, Joe Walker, Bill Dana, Bruce Peterson, Jack McKay, Milt Thompson, and Stan Butchart. of the group, Armstrong, Walker, Dana, McKay and Thompson all flew the X-15. Bruce Peterson flew the M2-F2 and HL-10 lifting bodies, while Stan Butchart was the B-29 drop plane pilot for many of the D-558-II and X-1 series research aircraft.

  3. Appetite and gut peptide responses to exercise and calorie restriction. The effect of modest energy deficits.

    Science.gov (United States)

    Deighton, Kevin; Batterham, Rachel L; Stensel, David J

    2014-10-01

    Weight loss is the result of a sustained negative energy balance, which is typically achieved by decreasing food intake and/or increasing physical activity. Current evidence suggests that acute energy deficits of ~4820 kJ elicit contrasting homeostatic responses when induced by exercise and food restriction but the response to government-recommended energy deficits is unknown. Twelve healthy men (mean(SD): age 24(5) years, body mass index 23.8(2.7) kg⋅m(-2), maximum oxygen uptake 55.4(9.1) mL⋅kg(-1)⋅min(-1)) completed three 8 h trials (control (Con), exercise-induced energy deficit (Ex-Def) and food restriction (Food-Def)) separated by 1 week. Thirty minutes of cycling at 64.5(3.2)% of maximum oxygen uptake was performed in Ex-Def from 0 to 0.5 h, which induced an energy deficit of 1469(256) kJ. An equivalent energy deficit was induced in Food-Def (1478(275) kJ) by reducing the energy content of standardised test meals at 1 h and 4 h. Appetite ratings, acylated ghrelin and peptide YY3-36 concentrations were measured throughout each trial. An ad libitum meal was provided at 7 h. Appetite was higher in Food-Def than Ex-Def from 4 to 8 h (P = 0.033) and tended to be higher across the entire 8 h trial (P = 0.059). However, energy intake at the ad libitum meal did not differ between trials (P = 0.634; Con 4376 (1634); Food-Def 4481 (1846); Ex-Def 4217 (1850) kJ). Acylated ghrelin was not related to changes in appetite but plasma PYY3-36 concentrations were higher in Ex-Def than Food-Def (P exercise. Appetite responses were associated with changes in plasma PYY3-36 but not acylated ghrelin concentrations and did not influence subsequent energy intake. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Primary exploration of conditions for hypothermic preservation of rat hepatocyte spheroids

    Directory of Open Access Journals (Sweden)

    Hong-ling LIU

    2015-01-01

    Full Text Available Objective To optimize conditions for hypothermic preservation of rat hepatocyte spheroids without freezing in order to facilitate the application of biological artificial liver. Methods Rat hepatic cells were isolated by a two-step perfusion method, and hepatocyte spheroids formed after 48 hours of rocking culture in serum free medium (SFM. Spheroids were then maintained in rocking culture at 37℃ (control condition, or cold stored at 4℃ for 24 or 48 hours in four different cold storage solutions: SFM alone; SFM+1mmol/L deferoxamine (Def ; SFM+1μmol/L cyclosporin A (CsA; and SFM+1mmol/L Def+1μmol/L CsA. After culturing for another 4 or 5 days, survival rate, changes in ultrastructure, and the production of albumin and urea were observed. Results Cold-induced injury could be reduced significantly by the addition of the iron chelators Def and CsA. The function and structure of hepatocyte spheroids stored in SFM+Def+CsA or SFM+Def for 24 hours were similar to those in control conditions. But the function was significantly reduced after hypothermic preservation in SFM alone. After cold storage for 48 hours, the ultrastructure of hepatocyte spheroids obviously changed and the number of dead cells increased. The survival rate of hepatocyte spheroids stored in SFM+Def+CsA or SFM+Def was significantly higher than that stored in SFM or SFM+CsA(P0.05. Conclusions Hepatocyte spheroids tolerate 24 hours of cold storage with stable viability and function. Hypothermic preservation increases the availability of cell-based therapy for liver diseases. DOI: 10.11855/j.issn.0577-7402.2014.12.02

  5. Spatial memory and hippocampal plasticity are differentially sensitive to the availability of choline in adulthood as a function of choline supply in utero.

    Science.gov (United States)

    Wong-Goodrich, Sarah J E; Glenn, Melissa J; Mellott, Tiffany J; Blusztajn, Jan K; Meck, Warren H; Williams, Christina L

    2008-10-27

    Altered dietary choline availability early in life leads to persistent changes in spatial memory and hippocampal plasticity in adulthood. Developmental programming by early choline nutrition may determine the range of adult choline intake that is optimal for the types of neural plasticity involved in cognitive function. To test this, male Sprague-Dawley rats were exposed to a choline chloride deficient (DEF), sufficient (CON), or supplemented (SUP) diet during embryonic days 12-17 and then returned to a control diet (1.1 g choline chloride/kg). At 70 days of age, we found that DEF and SUP rats required fewer choices to locate 8 baited arms of a 12-arm radial maze than CON rats. When switched to a choline-deficient diet (0 g/kg), SUP rats showed impaired performance while CON and DEF rats were unaffected. In contrast, when switched to a choline-supplemented diet (5.0 g/kg), DEF rats' performance was significantly impaired while CON and SUP rats were less affected. These changes in performance were reversible when the rats were switched back to a control diet. In a second experiment, DEF, CON, and SUP rats were either maintained on a control diet, or the choline-supplemented diet. After 12 weeks, DEF rats were significantly impaired by choline supplementation on a matching-to-place water-maze task, which was also accompanied by a decrease in dentate cell proliferation in DEF rats only. IGF-1 levels were elevated by both prenatal and adult choline supplementation. Taken together, these findings suggest that the in utero availability of an essential nutrient, choline, causes differential behavioral and neuroplastic sensitivity to the adult choline supply.

  6. Quantitative levels of Deficiens and Globosa during late petal development show a complex transcriptional network topology of B function.

    Science.gov (United States)

    Manchado-Rojo, María; Delgado-Benarroch, Luciana; Roca, María J; Weiss, Julia; Egea-Cortines, Marcos

    2012-10-01

    The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation of transcription of DEFICIENS (DEF) and GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth and petal size are affected, plays a role in B function. Late events in petal morphogenesis such as development of conical cell area and scent emissions were reduced in co and def (nicotianoides) (def (nic) ), and absent in co def (nic) double mutants, suggesting a role for CO in petal identity. Expression of DEF was down-regulated in co but surprisingly GLO was not affected. We investigated the levels of DEF and GLO at late stages of petal development in the co, def (nic) and glo-1 mutants, and established a reliable transformation protocol that yielded RNAi-DEF lines. We show that the threshold levels of DEF or GLO required to obtain petal tissue are approximately 11% of wild-type. The relationship between DEF and GLO transcripts is not equal or constant and changes during development. Furthermore, down-regulation of DEF or GLO does not cause parallel down-regulation of the partner. Our results demonstrate that, at late stages of petal development, the B function transcriptional network topology is not based on positive autoregulation, and has additional components of transcriptional maintenance. Our results suggest changes in network topology that may allow changes in protein complexes that would explain the fact that not all petal traits appear early in development.

  7. Cytotoxicity of various chemicals and mycotoxins in fresh primary duck embryonic fibroblasts: a comparison to HepG2 cells.

    Science.gov (United States)

    Chen, Xi; Murdoch, Rhonda; Shafer, Daniel J; Ajuwon, Kolapo M; Applegate, Todd J

    2016-11-01

    To screen cost-effectively the overall toxicity of a sample, particularly in the case of food and feed ingredient quality control, a sensitive bioassay is necessary. With the wide variety of cytotoxicity assays, performance comparison between assays using different cells has become of interest. Fresh primary duck embryonic fibroblasts (DEF) were hypothesized to be a sensitive tool for in vitro cytotoxicity screening; cell viability of DEF in response to various cytotoxins was determined and compared with response of HepG2 cells. The IC50 values by the alamar blue assay in the DEF cells had a high correlation (R(2)  = 0.96) with those obtained in HepG2 cells. Within the same toxin, primary DEF yielded significantly lower IC50 values than that obtained from HepG2 cells using the MTT and alamar blue assay. Additionally, primary DEF responded to all mycotoxins tested using the alamar blue assay, while HepG2 was less sensitive, particularly at short exposure times. The estimated IC50 for aflatoxin B1 , fumonisins B1 and deoxynivalenol in DEF after 72 h incubation were 3.69, 4.19 and 1.26 μg ml(-1) , respectively. Results from the current study suggest that primary DEF are more sensitive to cytotoxins and mycotoxins compared to HepG2, and thus may have great potential as an effective tool for cytotoxicity assessment. The question remains whether in vitro IC50 values can accurately predict in vivo toxicity; however, the current study accentuates the need for further attention to identify sensitive cell models for in vitro cytotoxicity screening and subsequent exploration of species-specific prediction models for in vivo toxicity. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  8. Drug: D09176 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 91], Beauveria bassiana [TAX:176275] Same as: E00308 Bombycidae Silkworm infected Cordycepitaceae Beauveria bassiana Vuillemin.; Stan...dards for non-pharmacopoeial crude drugs Crude drugs [BR:br08305] Animals Insects D09176 Stiff silkworm PubChem: 96025856 ...

  9. 什么是ISO 9000族标准?

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    "ISO 9000族"是国际标准化组织(International Organization for Stan-dardization,简称 ISO)在1994年提出的概念,是指"由国际标准化组织质量管理和质量保证技术委员会(ISO/TC

  10. Ämblikmees ja tehnoloogilised ängid / James Thurlow ; tõlk. Vappu Thurlow

    Index Scriptorium Estoniae

    Thurlow, James

    2004-01-01

    Mängufilm "Ämblikmees 2" ("Spider Man 2") : režissöör Sam Raimi : peaosatäitja Tobey Maguire : Ameerika Ühendriigid 2004. Filmi aluseks olev Stan Lee ja Steve Ditko koomiks alustas ilmumist 1962. aastal

  11. Testing single-grain quartz OSL methods using sediment samples with independent age control from the Bordes-Fitte rockshelter (Roches d'Abilly site, Central France)

    DEFF Research Database (Denmark)

    Thomsen, Kristina Jørkov; Murray, Andrew Sean; Buylaert, Jan-Pieter;

    2016-01-01

    We present quartz single-grain dose distributions for four well-bleached and unmixed sediment samples with independent age control (22–48 ka), from the archaeologically important Bordes-Fitte rockshelter at Roches d'Abilly, France. This site has previously been dated using 14C AMS dating and stan...

  12. Coordinate-Dependent N-Mode Squeezing Transformations

    Institute of Scientific and Technical Information of China (English)

    ZHANG Wei; REN Gang; CHEN Xiang-Rong; SONG Tong-Qiang; CAI Ling-Cang; GOU Qing-Quan

    2008-01-01

    We introduce the coordinate-dependent N-mode squeezing transformation and show that it can be con-structed by the combination of two unitary transformations, a coordinate-dependent displacement followed by the stan-dard squeezed transformation. The properties of the corresponding N-mode squeezed states are also discussed.

  13. A Report to the President: Analytic Perspectives on the Science and Technology Issues Facing the Nation

    Science.gov (United States)

    2001-01-01

    Vulnerabilidades Del Lavado Del Dinero Por Medio De Las Tecnologias Emergentes Del Ciberespacio: Un Ejercicio De Base Caribeno. David A. Mussington, Peter...toring is required to comply with National Ambient Air Quality Stan- dards. As reported in Monitoring for Fine Particulate Matter, RAND found that the

  14. 77 FR 12501 - Changes in Flood Elevation Determinations

    Science.gov (United States)

    2012-03-01

    ... Herald. Martin Luther King Jr. Boulevard, Chapel Hill, NC 27514. Stanly City of Albemarle (11- November 3... SECURITY Federal Emergency Management Agency 44 CFR Part 65 Changes in Flood Elevation Determinations... to this determination for the listed communities. From the date of the second publication of...

  15. 78 FR 35634 - Ocean Transportation Intermediary License Applicants

    Science.gov (United States)

    2013-06-13

    ...: Stan Sing Lau, Vice President (QI), David Koch, CEO. Application Type: QI Change. Logistics Management..., President. Application Type: QI Change. Daisy Mae Concepcion V. Taleon dba DMT Global Logistics (NVO & OFF... Value International dba JP Global Logistics (NVO & OFF), 377 Oyster Point Blvd., Suite 18, South...

  16. The Comic Book Project

    Science.gov (United States)

    Bitz, Michael

    2008-01-01

    The Comic Book Project (CBP) celebrates its eighth anniversary this year. The project, which just originated with one school in New York City, had grown to encompass thousands of students nationwide. The goal of the CBP is not to create comics the "Marvel way" or to develop the next Stan Lee. Rather, the goal is to give students the opportunity to…

  17. 78 FR 41161 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of Filing of Advance...

    Science.gov (United States)

    2013-07-09

    ... included in the econometric models underlying STANS, time series of proportional changes in implied...; (ii) incorporating, into the set of risk factors whose behavior is included in the econometric models... enhancements in OCC's margin model for longer-tenor options (i.e., those options with at least three years...

  18. 75 FR 68392 - Self-Regulatory Organizations; The Options Clearing Corporation; Order Approving Proposed Rule...

    Science.gov (United States)

    2010-11-05

    ... substitutions and withdrawals of equity collateral within the STANS Monte Carlo simulations.\\7\\ \\7\\ OCC believes... margin assets in the form of securities may be incorporated into the Monte Carlo calculations as an... Theoretical Analysis and Numerical Simulations Risk Management Methodology November 1, 2010. I. Introduction...

  19. A Transatlantic Bargain for the 21st Century: The United States, Europe, and the Transatlantic Alliance

    Science.gov (United States)

    2013-09-01

    with Afghanistan’s neighbors, including Iran and Paki- stan. It was also music to the ears of many Europe- ans who had been frustrated by what they...prospect of increasing irrelevance.91 Such warnings were followed by more tangible signs that the mood in Washington was firmly shifting. In Janu

  20. The Comic Book Project

    Science.gov (United States)

    Bitz, Michael

    2008-01-01

    The Comic Book Project (CBP) celebrates its eighth anniversary this year. The project, which just originated with one school in New York City, had grown to encompass thousands of students nationwide. The goal of the CBP is not to create comics the "Marvel way" or to develop the next Stan Lee. Rather, the goal is to give students the opportunity to…

  1. The role of urine markers, white light cystoscopy and fluorescence cystoscopy in recurrence, progression and follow-up of non-muscle invasive bladder cancer

    NARCIS (Netherlands)

    Karaoglu, I.; Heijden, A.G. van der; Witjes, J.A.

    2014-01-01

    Non-muscle invasive bladder cancer (NMIBC) accounts for approximately 70 % of all bladder cancer cases and represents a heterogeneous pathological entity, characterized by a variable natural history and oncological outcome. The combination of cystoscopy and urine cytology is considered the gold stan

  2. Proceedings of the Second International Congress on Recent Developments in Air- and Structure-Borne Sound and Vibration (2nd) Held in Auburn University, Alabama on 4-6 March 1992. Volume 2

    Science.gov (United States)

    1992-03-06

    863 V3M Kazarov, V.G. Karadga. and A.S. Minakov, Central Aerohydrodynamics Institute ( TSAGI ), Russia STATISTICS OF REVERBERANT TRANSFER FUNCTIONS...CENTRIFUGAL PUMPS .................................. 1521 L. Cominemen, INCERC-Amoustic Laboratory, Romania A. Stan, Academia Romani, Romania A SIMPLE LASER

  3. Proceedings of the Second International Congress on Recent Developments in Air- and Structure-Borne Sound and Vibration (2nd) Held in Auburn University, Alabama on 4-6 March 1992. Volume 1

    Science.gov (United States)

    1992-03-06

    Kazarov, V.G. Karadgi, and A.S. Mirskov, Central Aerohydrodynamics Institute ( TSAGI ), Russia STATISTICS OF REVERBERANT TRANSFER FUNCTIONS...THE NORMAL OPERATION OF CENTRIFUGAL PUMPS ................................... 1521 L ComAnescu, INCERC-Acoustic Laboratory, Romania A. Stan, Academia...Romang, Romania A SIMPLE LASER DEVICE FOR NONCONTACT VIBRATIONAL AMPLITUDE MEASUREMENT OF SOLIDS

  4. 78 FR 36002 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of Filing of Proposed...

    Science.gov (United States)

    2013-06-14

    ... believes these results are consistent with what would be expected given the strong negative correlation... prices used by STANS to value each portfolio indicative daily quotations obtained through a third- party... options may represent a larger portion of any clearing member's portfolio in the future, and OCC has...

  5. Valgdeltagelse og demokratisk legitimitet

    DEFF Research Database (Denmark)

    Rostbøll, Christian F.; Dabelsteen, Hans Boas

    2013-01-01

    Den meget lave deltagelse til menighedsrådsvalgene i den danske folkekirke rejser spørgsmålet, hvor vigtig høj valgdeltagelse er for demokratisk legitimitet. Artiklen diskuterer dette spørgsmål på baggrund af den politiske idehistorie og nutidens demokratiteori. Der opstilles nogle normative stan...

  6. ST-analysis in electronic foetal monitoring is cost-effective from both the maternal and neonatal perspective

    NARCIS (Netherlands)

    van ’t Hooft, Janneke; Vink, Maarten; Opmeer, Brent C.; Ensing, Sabine; Kwee, Anneke; Mol, Ben Willem J

    2016-01-01

    Objective: Electronic foetal monitoring (EFM) together with non-invasive ST-analysis (STAN) has been suggested as a superior technique to EFM alone for foetal surveillance to prevent metabolic acidosis. This study aims to compare the cost-effectiveness of these two techniques from both maternal (sho

  7. Multi-laboratory study of the analysis and kinetics of stanozolol and its metabolites in treated calves

    NARCIS (Netherlands)

    Brabander, H.F. de; Wasch, K. de; Ginkel, L.A. van; Sterk, S.S.; Blokland, M.H.; Delahaut, Ph.; Taillieu, X.; Dubois, M.; Arts, C.J.M.; Baak, M.J. van; Gramberg, L.G.; Schilt, R.; Bennekom, E.O. van; Courtheyn, D.; Vercammen, J.; Witkamp, R.F.

    1998-01-01

    The European Union banned the use of anabolic steroids for cattle fattening in 1988. Analytical techniques able to detect trace amounts of the parent drugs and their metabolites are mandatory for the control of abuse. Stanozolol (Stan) is an anabolic steroid that is often found in injection sites an

  8. Microcomputer Software System Development: Suggested Revisions to MIL-STD-1521A for Cost-Effective Acquisition of Custom Software through Software Engineering.

    Science.gov (United States)

    1983-09-01

    April 1981, pp. 139-151. 41. Schiffres, Manuel . "Behind the Shakeup in Personal Computers," U.S. News and World Report, Vol. 94, No. 25 (27 June 1983...Command. A Guide for Program Management. AFSCP 800-3. Wright-Patterson AFB OH, 9 April 1976. Bersoff, Edward, Vilas D. Henderson, and Stan Sugel. "Software

  9. Demography of European Honey Buzzards Pernis apivorus

    NARCIS (Netherlands)

    Bijlsma, Rob G.; Vermeulen, Minou; Hemerik, Lia; Klok, Chris

    2012-01-01

    Bijlsma R.G., Vermeulen M., Hemerik L. & Klok C. 2012. Demography of European Honey Buzzards Pernis apivorus. Ardea 100: 163-177. We set out to explore whether changes in fecundity and survival (or both) of European Honey Buzzards were associated with trends observed in The Netherlands. We used stan

  10. Evolution of natural history information in the 21st Century - Developing an integrated framework for biological and geographic data

    Science.gov (United States)

    Threats to marine and estuarine species operate over many spatial scales, from nutrient enrichment at the watershed/estuarine scale to invasive species and climate change at regional and global scales. To help address research questions across these scales, we provide here a stan...

  11. Analytical possibilities for the detection of stanozolol and its metabolites

    NARCIS (Netherlands)

    Poelmans, S.; Wasch, K. de; Brabander, H.F. de; Wiele, M. van de; Courtheyn, D.; Ginkel, L.A. van; Sterk, S.S.; Delahaut, Ph.; Dubois, M.; Schilt, R.; Nielen, M.; Vercammen, J.; Impens, S.; Stephany, R.; Hamoir, T.; Pottie, G.; Poucke, C. van; Peteghem, C. van

    2002-01-01

    In sports doping, as well in man as in horseracing, stanozolol (Stan) was abused and became the subject of metabolism research. Also in veterinary practice, stanozolol became an important misused anabolic steroid. Like most other anabolic steroids, stanozolol has poor gas chromatographic behavior. I

  12. Multi-laboratory study of the analysis and kinetics of stanozolol and its metabolites in treated calves

    NARCIS (Netherlands)

    Brabander, H.F. de; Wasch, K. de; Ginkel, L.A. van; Sterk, S.S.; Blokland, M.H.; Delahaut, Ph.; Taillieu, X.; Dubois, M.; Arts, C.J.M.; Baak, M.J. van; Gramberg, L.G.; Schilt, R.; Bennekom, E.O. van; Courtheyn, D.; Vercammen, J.; Witkamp, R.F.

    1998-01-01

    The European Union banned the use of anabolic steroids for cattle fattening in 1988. Analytical techniques able to detect trace amounts of the parent drugs and their metabolites are mandatory for the control of abuse. Stanozolol (Stan) is an anabolic steroid that is often found in injection sites an

  13. Area Handbook Series: Finland: A Country Study

    Science.gov (United States)

    1988-12-01

    Peggy Pixley. David P. Cabitto, who was assisted by Sandra K. Cotugno and Kimberly A. Lord, provided invaluable graphics support. Stan- ley M. Sciora...Right Wing in Finland (Rus- sian and East European Series, 22.). Bloomington: Indiana University Press, 1962. Runeberg, Carl Michael. Finlands Historia

  14. En påle genom Dracula-filmernas hjärta? : En komparativ analys av adaptationer av Bram Stokers Dracula (1897) från åren 2000-2014

    OpenAIRE

    Bahrman, Alexander

    2016-01-01

    En komparativ analys av adaptationer av Bram Stokers Dracula (1897) från åren 2000-2014 med syfte att undersöka resultatet av nästan 100 år av adaptationer och ett mål för att verkställa om en filmkanon har skapats kring karaktären.

  15. Mummy Berry Fruit Rot and Shoot Blight Incidence in Blueberry: Prediction, Ranking, and Stability in a Long-term Study

    Science.gov (United States)

    Mummy berry is an important disease of cultivated blueberry. The disease has two distinct phases; a blighting phase initiated by ascospores and a fruit infection stage initiated by conidia. In this study we investigated blueberry cultivar resistance to both phases of the disease and, utilizing ‘stan...

  16. Early aberrations in chromatin dynamics in embryos produced under In vitro conditions

    DEFF Research Database (Denmark)

    Deshmukh, Rahul Shahaji; Østrup, Olga; Strejcek, Frantisek

    2012-01-01

    In vitro production of porcine embryos by means of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) is limited by great inefficienciy. The present study investigated chromatin and nucleolar dynamics in porcine embryos developed in vivo (IV) and compared this physiological stan...

  17. The 12th Annual International Meeting on Simulation Healthcare (IMSH) 2012

    Science.gov (United States)

    2012-03-01

    Linebacker Stan: An Interprofessional Simulation Room: 28E 5PP.380Int Podium Presentation Course Overview: On-field care of a spine injured football ... Neuroscience Course for Medical and Dental Students Sub Content: Medical Education (undergraduate graduate) Session 2:1 Assigned Professor

  18. PR: Priority for Community College Libraries.

    Science.gov (United States)

    Vaughan, Elinor F.

    1987-01-01

    Describes the Stanly Technical College Library's public relations program which attempts to encourage the use of library and media resources and to promote the college as a major community resource. Highlights library activities, including a photography contest for students and staff, and displays featuring college programs and local history. (DMM)

  19. Water Quality Standards for Coral Reef Protection

    Science.gov (United States)

    The U.S. Clean Water Act provides a legal framework to protect coastal biological resources such as coral reefs, mangrove forests, and seagrass meadows from the damaging effects of human activities. Even though many resources are protected under this authority, water quality stan...

  20. MR compatible strain gauge based force transducer

    NARCIS (Netherlands)

    van Duinen, Hiske; Post, Marijn; Vaartjes, Koen; Hoogduin, Hans; Zijdewind, Inge

    2007-01-01

    In order to evaluate brain activation during motor tasks accurately one must also measure output parameters such as muscle force or muscle activity. Especially in clinical situations where the force output can be compromised by changes at different levels of the motor system, it is essential to stan

  1. Stochastic Geometry and Topology of Non-Gaussian Fields

    NARCIS (Netherlands)

    Beuman, T.H.; Turner, A.M.; Vitelli, V.

    2012-01-01

    Gaussian random fields pervade all areas of science. However, it is often the departures from Gaussianity that carry the crucial signature of the nonlinear mechanisms at the heart of diverse phenomena, ranging from structure formation in condensed matter and cosmology to biomedical imaging. The stan

  2. Analytical possibilities for the detection of stanozolol and its metabolites

    NARCIS (Netherlands)

    Poelmans, S.; Wasch, K. de; Brabander, H.F. de; Wiele, M. van de; Courtheyn, D.; Ginkel, L.A. van; Sterk, S.S.; Delahaut, Ph.; Dubois, M.; Schilt, R.; Nielen, M.; Vercammen, J.; Impens, S.; Stephany, R.; Hamoir, T.; Pottie, G.; Poucke, C. van; Peteghem, C. van

    2002-01-01

    In sports doping, as well in man as in horseracing, stanozolol (Stan) was abused and became the subject of metabolism research. Also in veterinary practice, stanozolol became an important misused anabolic steroid. Like most other anabolic steroids, stanozolol has poor gas chromatographic behavior. I

  3. Ämblikmehe uus tulemine / Laurence Lumiere

    Index Scriptorium Estoniae

    Lumiere, Laurence

    2004-01-01

    Mängufilm "Ämblikmees 2" ("Spider Man 2") : režissöör Sam Raimi : peaosatäitja Tobey Maguire : Ameerika Ühendriigid 2004. Filmi aluseks olev Stan Lee ja Steve Ditko koomiks alustas ilmumist 1962. aastal

  4. Water Accounting Plus for Water Resources Reporting and River Basin Planning

    NARCIS (Netherlands)

    Karimi, P.

    2014-01-01

    This thesis introduces Water Accounting Plus (WA+), which is a new framework designed to provide explicit spatial information on water depletion and net withdrawal processes in complex river basins. WA+ is a simple, yet comprehensive and understandable water accounting framework that provides a stan

  5. Sitcoms.

    Science.gov (United States)

    Berman, Ronald

    1987-01-01

    Examines humorous content of today's television sitcoms in attempt to explain how we got from "Stan and Ollie to Shirley and Laverne" or from exemplary humor to the mundane social commentary of present-day situational comedy. Concludes that the sitcom depends on making comedy out of collision between social change and tradition, exploiting social…

  6. Ämblikmehe uus tulemine / Laurence Lumiere

    Index Scriptorium Estoniae

    Lumiere, Laurence

    2004-01-01

    Mängufilm "Ämblikmees 2" ("Spider Man 2") : režissöör Sam Raimi : peaosatäitja Tobey Maguire : Ameerika Ühendriigid 2004. Filmi aluseks olev Stan Lee ja Steve Ditko koomiks alustas ilmumist 1962. aastal

  7. Ämblikmees ja tehnoloogilised ängid / James Thurlow ; tõlk. Vappu Thurlow

    Index Scriptorium Estoniae

    Thurlow, James

    2004-01-01

    Mängufilm "Ämblikmees 2" ("Spider Man 2") : režissöör Sam Raimi : peaosatäitja Tobey Maguire : Ameerika Ühendriigid 2004. Filmi aluseks olev Stan Lee ja Steve Ditko koomiks alustas ilmumist 1962. aastal

  8. How life expectancy varies with perturbations in age-specific mortality

    DEFF Research Database (Denmark)

    Wrycza, Tomasz F.; Baudisch, Annette

    2012-01-01

    and resulting change in e 0, and we systematically apply it to investigate the effect of specific mortality perturbations. COMMENTS: Expressions for the change in e 0 resulting from a change in the parameters of the stan-dard parametric mortality model in demography, the Gompertz-Makeham model, include well...

  9. MEMBRANE-TRANSPORT OF METHOTREXATE IN A SQUAMOUS CARCINOMA CELL-LINE ADAPTED TO LOW FOLATE CONCENTRATIONS

    NARCIS (Netherlands)

    van der Laan, B.F.A.M.; Jansen, G; VANGESTEL, JA; SCHORNAGEL, JH; HORDIJK, GJ

    1991-01-01

    Membrane transport characteristics of the folate analogue methotrexate (MTX) were studied in a human squamous carcinoma cell line of the head and neck (HNSCC) adapted to grow in tissue culture media with nanomolar reduced folate concentrations (SCC-11B-LF), as compared to SCC-11B cells grown in stan

  10. Setting the Standard

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The country’s self-developed document format standard faces a difficult decision and possible extinction as it goes up against mighty Microsoft Since Microsoft’s Office Open Extensible Markup Language (OOXML)document format was accepted as an international stan- dard by the International Organization for

  11. Shape and Doppler correction for a towed sonar array

    NARCIS (Netherlands)

    Groen, J.; Beerens, S.P.; Doisy, Y.

    2004-01-01

    Anti Submarine Warfare (ASW) is more and more focused toward shallow water environments. Many complications have come up for ASW sonar performance as a result of this. The problem that is tackled in this article is the performance loss due to the shape and motion of the sonar during a maneuver. Stan

  12. SIMULATION OF NON-AZEOTROPIC REFRIGERANT MIXTURES FOR USE IN A DUAL-CIRCUIT REFRIGERATOR/FREEZER WITH COUNTERCURRENT HEAT EXCHANGES

    Science.gov (United States)

    The paper discusses a refrigerator/freezer (RF) system that has two complete and independent refrigeration cycles for the two compartments. It uses a non-azeotropic refrigerant mixture (NARM) in each cycle and countercurrent heat exchangers throughout. This RF is housed in a stan...

  13. Amerikantsõ ljubjat Rossiju bez vzaimnosti

    Index Scriptorium Estoniae

    2008-01-01

    Avaldati maailmale positiivset ja negatiivset mõju omavate riikide reitingud, nii Venemaa kui USA on oma mainet rahvusvahelisel areenil parandanud. Küsitluse viis läbi BBC koostöös sotsioloogiliste teenistustega GlobeStan ja PIPA

  14. 3D Imaging Technology’s Narrative Appropriation in Cinema

    NARCIS (Netherlands)

    Kiss, Miklós; van den Oever, Annie; Fossati, Giovanna

    2016-01-01

    This chapter traces the cinematic history of stereoscopy by focusing on the contemporary dispute about the values of 3D technology, which are seen as either mere visual attraction or as a technique that perfects the cinematic illusion through increasing perceptual immersion. By taking a neutral stan

  15. En påle genom Dracula-filmernas hjärta? : En komparativ analys av adaptationer av Bram Stokers Dracula (1897) från åren 2000-2014

    OpenAIRE

    Bahrman, Alexander

    2016-01-01

    En komparativ analys av adaptationer av Bram Stokers Dracula (1897) från åren 2000-2014 med syfte att undersöka resultatet av nästan 100 år av adaptationer och ett mål för att verkställa om en filmkanon har skapats kring karaktären.

  16. A computationally efficient depression-filling algorithm for digital elevation models, applied to proglacial lake drainage

    NARCIS (Netherlands)

    Berends, Constantijn J.; Van De Wal, Roderik S W

    2016-01-01

    Many processes govern the deglaciation of ice sheets. One of the processes that is usually ignored is the calving of ice in lakes that temporarily surround the ice sheet. In order to capture this process a "flood-fill algorithm" is needed. Here we present and evaluate several optimizations to a stan

  17. The influence of elevated preovulatory estradiol on apoptosis in the trophectoderm of day 16 bovine conceptuses

    Science.gov (United States)

    Preovulatory estradiol impacts follicular growth, oocyte maturation, sperm transport, uterine environment, and embryo survival/development. It has also been reported that cows in standing estrus within 24 hours of fixed-time AI have greater pregnancy success compared to cows that do not exhibit stan...

  18. Individualism and Marginality: From Comic Book to Film--Marvel Comics Superheroes

    Science.gov (United States)

    Tuzi, Marino

    2005-01-01

    In this article, the author offers his perspective that underlies the plot lines and character developments in various super-hero serials produced by Marvel Comic Books. Stan Lee, the creator of this giant comic books company, the original writers and artists, and their successors at Marvel Comics have given readers a vision of human reality that…

  19. 75 FR 55619 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of Filing of Proposed...

    Science.gov (United States)

    2010-09-13

    ... assets on a portfolio basis with reference to the volatility and correlation of each deposited security..., OCC believes analyzing cleared positions and margin assets as a single portfolio using STANS provides... should this change be implemented. As a part of this proposal, OCC would apply a portfolio specific...

  20. QTL Information Table: 512 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available qBLASTa-12-2f Resistance or Tolerance Blast resistance the area under a disdisease ...progress curve RFLP C)Interval RIL Lemont Teqing A RG574 pha Tabien, E., Li, Z., Paterson, H., Marchetti, A., Stan...sel, W., and Pinson, M. (2002). Mapping QTLs for field resistance to the rice blast pathogen and evalu

  1. QTL Information Table: 511 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available qBLASTads-2 Resistance or Tolerance Blast resistance Standard Evaluation System (SE...S) for rating leaf blast RFLP C)Interval RIL Lemont Teqing A RG520 pha Tabien, E., Li, Z., Paterson, H., Marchetti, A., Stan...sel, W., and Pinson, M. (2002). Mapping QTLs for field resistance to the rice blast pathogen

  2. On the standardisation of Web service management operations

    NARCIS (Netherlands)

    Sloten, van J.; Pras, A.; Sinderen, van M.J.; Harju, J.; Moltchanov, D.; Silverajan, B.

    2004-01-01

    Given the current interest in TCP/IP network management research towards Web services, it is important to recognise how standardisation can be achieved. This paper mainly focuses on the standardisation of operations and not management information. We state that standardisation should be done by stan

  3. "Boys Don't Do Dance, Do They?"

    Science.gov (United States)

    Holdsworth, Nadine

    2013-01-01

    In 2005, the Warwick Arts Centre launched the "Boys Dancing" project through the formation of the West Midlands Boys Dance Alliance. Aimed exclusively at boys and young men, the project has offered a range of performance-making opportunities with male professionals including Liam Steel (DV8, Stan Won't Dance) and David McKenna…

  4. Climatology of Wind Direction Fluctuations at Risø

    DEFF Research Database (Denmark)

    Kristensen, Leif; Panofsky, H. A.

    1976-01-01

    Standard deviations of wind direction fluctuations at 76 m at Risø for the first half year of 1975 have been analyzed as functions of wind speed and temperature lapse rate, either measured near the surface or near the level of the azimuth variations. Between 31 and 37% of the variance of the stan...

  5. Polish Martial Law: The Crisis of Communism

    Science.gov (United States)

    2009-12-01

    Academy, 2001 Submitted in partial fulfillment of the requirements for the degree of MASTER OF ARTS IN SECURITY STUDIES (EUROPE, EURASIA...they going in? They did not,” Woodrow Wilson International Center for Scholars, http://www.wilsoncenter.org/news/ docs/ Historia -Jachranka%20-%20Stan

  6. MR compatible strain gauge based force transducer

    NARCIS (Netherlands)

    van Duinen, Hiske; Post, Marijn; Vaartjes, Koen; Hoogduin, Hans; Zijdewind, Inge

    2007-01-01

    In order to evaluate brain activation during motor tasks accurately one must also measure output parameters such as muscle force or muscle activity. Especially in clinical situations where the force output can be compromised by changes at different levels of the motor system, it is essential to stan

  7. Fungsi Bangunan Dokwi Vam dan Kembu Vam Bagi Suku Yali dalam Novel Penguasa-penguasa Bumi Karya Don Richardson

    Directory of Open Access Journals (Sweden)

    Ummu Fatimah Ria Lestari

    2016-04-01

    Full Text Available This study discusses the function of the building of Dokwi Vam and Kembu Vam contained in the novel-Sovereign Ruler of the Earth works Don Richardson. In general, this novel tells the story of the life of Stan Dale and Yali tribe. Stan Dale is a missionary who served in Papua. He struggled to introduce Christianity to the Yali tribe. This study uses the description of the technical literature. This research resulted in a description of the function of the building of Dokwi Vam and Kembu Vam contained in the novel Lord of-Ruler of the Earth works Don Richardson. Dokwi Vam used as a museum (where the old stuff to worship as they still follow animism, while the Kembu Vam serves as a temple / animism worship in Yali tribe. Penelitian ini membahas tentang fungsi bangunan Dokwi Vam dan Kembu Vam yang terdapat dalam novel Penguasa-Penguasa Bumi karya Don Richardson. Secara umum, novel ini bercerita tentang kehidupan Stan Dale dan suku Yali. Stan Dale adalah seorang missionaris yang melayani di Tanah Papua. Ia berjuang untuk memperkenalkan agama Nasrani kepada suku Yali. Penelitian ini menggunakan metode deskripsi dengan teknik studi pustaka. Penelitian ini menghasilkan deskripsi tentang fungsi bangunan Dokwi Vam dan Kembu Vam yang terdapat dalam novel Penguasa-Penguasa Bumi karya Don Richardson. Dokwi Vam digunakan sebagai museum (tempat barang-barang kuno untuk penyembahan karena mereka masih menganut kepercayaan animisme, sedangkan Kembu Vam berfungsi sebagai rumah peribadatan/penyembahan dalam kepercayaan animisme suku Yali.

  8. F-35A Training Basing Environmental Impact Statement. Volume 2. Appendix A through Appendix C

    Science.gov (United States)

    2012-06-01

    Fran Rosker David Ross Donnie Ross John Ross Sharon Ross Donna Rounds Todd Rounds Glen Ruark Judy Ruark Laura Sagerman Maggie Samuelson ...Martin Samuelson Norberta Santiago Stan W. Sapkos Ash Scheder Black John Schell Charles Schep Ed Schmit Peter Schmugge Jamie Schremmer Edgar

  9. A Family of CSαβ Defensins and Defensin-Like Peptides from the Migratory Locust, Locusta migratoria, and Their Expression Dynamics during Mycosis and Nosemosis

    Science.gov (United States)

    Zhang, Liwei; Zhang, Pengfei; Zhang, Long

    2016-01-01

    Insect defensins are effector components of the innate defense system. During infection, these peptides may play a role in the control of pathogens by providing protective antimicrobial barriers between epithelial cells and the hemocoel. The cDNAs encoding four defensins of the migratory locust, Locusta migratoria, designated LmDEF 1, 3–5, were identified for the first time by transcriptome-targeted analysis. Three of the members of this CSαβ defensin family, LmDEF 1, 3, and 5, were detected in locust tissues. The pro regions of their sequences have little-shared identities with other insect defensins, though the predicted mature peptides align well with other insect defensins. Phylogenetic analysis indicates a completely novel position of both LmDEF 1 and 3, compared to defensins from hymenopterans. The expression patterns of the genes encoding LmDEFs in the fat body and salivary glands were studied in response to immune-challenge by the microsporidian pathogen Nosema locustae and the fungus Metarhizium anisopliae after feeding or topical application, respectively. Focusing on Nosema-induced immunity, qRT-PCR was employed to quantify the transcript levels of LmDEFs. A higher transcript abundance of LmDEF5 was distributed more or less uniformly throughout the fat body along time. A very low baseline transcription of both LmDEFs 1 and 3 in naïve insects was indicated, and that transcription increases with time or is latent in the fat body or salivary glands of infected nymphs. In the salivary glands, expression of LmDEF3 was 20-40-times higher than in the fat body post-microbial infection. A very low expression of LmDEF3 could be detected in the fat body, but eventually increased with time up to a maximum at day 15. Delayed induction of transcription of these peptides in the fat body and salivary glands 5–15 days post-activation and the differential expression patterns suggest that the fat body/salivary glands of this species are active in the immune response

  10. A Family of CSαβ Defensins and Defensin-Like Peptides from the Migratory Locust, Locusta migratoria, and Their Expression Dynamics during Mycosis and Nosemosis.

    Science.gov (United States)

    Lv, Mingyue; Mohamed, Amr Ahmed; Zhang, Liwei; Zhang, Pengfei; Zhang, Long

    2016-01-01

    Insect defensins are effector components of the innate defense system. During infection, these peptides may play a role in the control of pathogens by providing protective antimicrobial barriers between epithelial cells and the hemocoel. The cDNAs encoding four defensins of the migratory locust, Locusta migratoria, designated LmDEF 1, 3-5, were identified for the first time by transcriptome-targeted analysis. Three of the members of this CSαβ defensin family, LmDEF 1, 3, and 5, were detected in locust tissues. The pro regions of their sequences have little-shared identities with other insect defensins, though the predicted mature peptides align well with other insect defensins. Phylogenetic analysis indicates a completely novel position of both LmDEF 1 and 3, compared to defensins from hymenopterans. The expression patterns of the genes encoding LmDEFs in the fat body and salivary glands were studied in response to immune-challenge by the microsporidian pathogen Nosema locustae and the fungus Metarhizium anisopliae after feeding or topical application, respectively. Focusing on Nosema-induced immunity, qRT-PCR was employed to quantify the transcript levels of LmDEFs. A higher transcript abundance of LmDEF5 was distributed more or less uniformly throughout the fat body along time. A very low baseline transcription of both LmDEFs 1 and 3 in naïve insects was indicated, and that transcription increases with time or is latent in the fat body or salivary glands of infected nymphs. In the salivary glands, expression of LmDEF3 was 20-40-times higher than in the fat body post-microbial infection. A very low expression of LmDEF3 could be detected in the fat body, but eventually increased with time up to a maximum at day 15. Delayed induction of transcription of these peptides in the fat body and salivary glands 5-15 days post-activation and the differential expression patterns suggest that the fat body/salivary glands of this species are active in the immune response

  11. Correlations Between Supermassive Black Holes, Velocity Dispersions, and Mass Deficits in Elliptical Galaxies with Cores

    CERN Document Server

    Kormendy, John; 10.1088/0004-637X/691/2/L142

    2009-01-01

    High-dynamic-range surface photometry in a companion paper makes possible accurate measurement of the stellar light deficits L_def and mass deficits M_def associated with the cores of elliptical galaxies. We show that L_def correlates with the velocity dispersion sigma of the host galaxy bulge averaged outside the central region that may be affected by a supermassive black hole (BH). We confirm that L_def correlates with BH mass MBH. Also, the fractional light deficit L_def/L correlates with MBH/M, the ratio of BH mass to the galaxy stellar mass. All three correlations have scatter similar to or smaller than the scatter in the well known correlation between MBH and sigma. The new correlations are remarkable in view of the dichotomy between ellipticals with cores and those with central extra light. Core light deficit correlates closely with MBH and sigma, but extra light does not. This supports the suggestion that extra light Es are made in wet mergers with starbursts whereas core Es are made in dry mergers. A...

  12. Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae

    Directory of Open Access Journals (Sweden)

    MG Parisi

    2015-10-01

    Full Text Available Immunological effectors of invasive species playing a role in addressing new colonization are still poorly studied. In the present study the cDNA sequence of the defensin from a Lessepsian invasive species, the Red Sea mussel Brachidontes pharaonis, was cloned using RACE method. Defensins are a class of widely known antimicrobial peptides (AMPs, oligopeptides with a broad spectrum of targeted organisms ranging from viruses to parasites. Analysis of BpDef sequence (262 bp revealed the presence of an ORF coding for 81 amino acids. The full-length amino acid sequence showed the highest similarity to antimicrobial peptides MGD1 and MGD2 sequence from Mytilus galloprovincialis. Phylogenetic analysis suggested that BpDef belongs to the αβ defensin AMPs with a typical domain structurally characterized by a α helix and two β sheets. BpDef mRNA is located in circulating hemocytes with small intra-cytoplasmic granules and with large granules. The transcription of defensin gene was modulated by the stress from temperatures and oxygenation condition. Temperatures of 20 °C did not stimulate a BpDef transcription over a short time. At 30 °C the kinetics of BpDef gene transcription showed up regulation after one day, while it was down regulated after six days, both under normoxia and hypoxia conditions.

  13. The effect of dietary Cu and diabetes on indices of Cu nutriture in the rat

    Energy Technology Data Exchange (ETDEWEB)

    Rucker, R.B.; Uriu-Hare, J.Y.; Tinker, D.; Keen, C.L. (Univ. of California, Davis (United States))

    1991-03-11

    The uptake-retention of 67Cu is affected by dietary Cu and diabetes. Consequently, the functional activities of select enzymes and tissue Cu status were assessed. STZ-diabetic and control rats were fed Cu suppl. or def. diets. Rats were gavaged with 28 {mu}Ci 67Cu, and killed 8, 16, 24, 32, 64, or 128 h later. Diabetic rats were hyperphagic, hyperglycemic and hypoinsulinemic; with no effect of diet. Plasma ceruloplasmin activity (Cp) was lower in Cu def. rats; diabetic rats tended to have higher Cp than controls. Cu def. rats had low Cu levels in liver, kidney and plasma. Cu suppl. diabetic rats had higher liver and kidney Cu compared to Cu def. diabetic rats. Gel chromatography of liver showed that with time, there was a transfer of 67Cu from low to higher MW ligands. In nondiabetic rats, more 67Cu was associated with the higher MW ligands. The converse was observed for diabetic rats. There was no effect of diabetes on liver 67Cu localization. Diabetic rats had higher metallothionein (MT) concentrations in liver and kidney compared to controls Cu deficiency lowered MT values in both diabetic and control rats. CuZn SOD Cu activity was lowered with Cu def. and diabetes, while Mn SOD activity was similar among groups. Plasma lipid peroxide levels were lower in diabetic rats than controls. The results show that Cu metabolism is affected in diabetes, and the changes are functionally significant.

  14. The role of hexon in egg drop syndrome virus (EDSV) inducing apoptosis in duck embryo fibroblast cells.

    Science.gov (United States)

    Qi, Xuefeng; Xu, Jiamin; Wang, Zugui; Wang, Xueping; Wang, Jingyu

    2017-07-17

    Although extensive efforts have been made to understand adenovirus infection in human cells, little is known for egg drop syndrome virus (EDSV) infection in the avian-derived cells. In this study, the effects of EDSV infection as well as the possible role hexon protein, the main building block of the EDSV capsid, on apoptosis induction in duck embryo fibroblast (DEF) cells was examined. Flow cytometry analysis and TUNEL assay revealed that EDSV infection induced significant apoptosis in DEF cells compared with mock infected cells. Interestingly, the increase of the apoptosis rate detected in EDSV infected DEF cells were accompanied by an increased virus load in cells in a time-dependent manner. Furthermore, a time-dependent decrease in hexon protein expression levels in hexon transfected DEF cells in parallel with a gradual decrease in TUNEL-labeling cells was also observed in the current study. In addition, caspase activity detection and western blot analysis indicates that either EDSV infection or EDSV hexon transfection both induced apoptosis of DEF cells via activating both the exogenous and the mitochondrial pathway. Copyright © 2017. Published by Elsevier Ltd.

  15. Effect of Photon Beam Energy, Gold Nanoparticle Size and Concentration on the Dose Enhancement in Radiation Therapy

    Directory of Open Access Journals (Sweden)

    Nahideh Gharehaghaji

    2013-02-01

    Full Text Available Introduction: Gold nanoparticles have been used as radiation dose enhancing materials in recent investigations. In the current study, dose enhancement effect of gold nanoparticles on tumor cells was evaluated using Monte Carlo (MC simulation. Methods: We used MCNPX code for MC modeling in the current study. A water phantom and a tumor region with a size of 1×1×1 cm3 loaded with gold nanoparticles were simulated. The macroscopic dose enhancement factor was calculated for gold nanoparticles with sizes of 30, 50, and 100 nm. Also, we simulated different photon beams including mono-energetic beams (50-120 keV, a Cobalt-60 beam, 6 & 18 MV photon beams of a conventional linear accelerator. Results: We found a dose enhancement factor (DEF of from 1.4 to 3.7 for monoenergetic kilovoltage beams, while the DEFs for megavoltage beams were negligible and less than 3% for all GNP sizes and concentrations. The optimum energy for higher DEF was found to be the 90 keV monoenergetic beam. The effect of GNP size was not considerable, but the GNP concentration had a substantial impact on achieved DEF in GNP-based radiation therapy. Conclusion: The results were in close agreement with some previous studies considering the effect of photon energy and GNP concentration on observed DEF. Application of GNP-based radiation therapy using kilovoltage beams is recommended.

  16. Type III interferon gene expression in response to influenza virus infection in chicken and duck embryonic fibroblasts.

    Science.gov (United States)

    Zhang, Zhijie; Zou, Tingting; Hu, Xiaotong; Jin, Hong

    2015-12-01

    Type III interferons (IFN-λs) comprise a group of newly identified antiviral cytokines that are functionally similar to type I IFNs and elicit first-line antiviral responses. Recently, type III IFNs were identified in several species; however, little information is available about type III IFNs in ducks. We compared the expression of type III IFNs and their receptor in chicken embryonic fibroblasts (CEFs) and duck embryonic fibroblasts (DEFs) in response to influenza virus infection. The results showed that the expression of type III IFNs was upregulated in both DEFs and CEFs following infection with H1N1 influenza virus or treatment with poly (I:C), and expression levels were significantly higher in CEFs than in DEFs at each time point. The expression of the receptor for type III IFNs (IL-28Rα) was also upregulated following infection with H1N1 virus or treatment with poly (I:C) and was significantly higher in CEFs than in DEFs at each time point. The expression of the receptor for type III IFNs occurred from 8 hpi and remained at similar levels until 36 hpi in CEFs, but the expression level was elevated from 36 hpi in DEFs. These findings revealed the existence of distinct expression patterns for type III IFNs in chickens and ducks in response to influenza virus infection. The provided data are fundamentally useful in furthering our understanding of type III IFNs and innate antiviral responses in different species.

  17. An anionic defensin from Plutella xylostella with potential activity against Bacillus thuringiensis.

    Science.gov (United States)

    Xu, X-X; Zhang, Y-Q; Freed, S; Yu, J; Gao, Y-F; Wang, S; Ouyang, L-N; Ju, W-Y; Jin, F-L

    2016-12-01

    Insect defensins, are cationic peptides that play an important role in immunity against microbial infection. In the present study, an anionic defensin from Plutella xylostella, (designated as PxDef) was first cloned and characterized. Amino acid sequence analysis showed that the mature peptide owned characteristic six-cysteine motifs with predicted isoelectric point of 5.57, indicating an anionic defensin. Quantitative real-time polymerase chain reaction analysis showed that PxDef was significantly induced in epidermis, fat body, midgut and hemocytes after injection of heat-inactivated Bacillus thuringiensis, while such an induction was delayed by the injection of live B. thuringiensis in the 4th instar larvae of P. xylostella. Knocking down the expression of nuclear transcription factor Dorsal in P. xylostella by RNA interference significantly decreased the mRNA level of PxDef, and increased the sensitivity of P. xylostella larvae to the infection by live B. thuringiensis. The purified recombinant mature peptide (PxDef) showed higher activity against Gram-positive bacteria, with the minimum inhibition concentrations of 1.6 and 2.6 µM against B. thuringiensis and Bacillus subtilis, respectively. To our knowledge, this is the first report about an anionic PxDef, which may play an important role in the immune system of P. xylostella against B. thuringiensis.

  18. Prevalence, risk surfaces and inter-municipality variations in caries experience in Danish children and adolescents in 2012

    DEFF Research Database (Denmark)

    Nørrisgaard, Pia Elisabeth; Qvist, Vibeke; Ekstrand, Kim

    2016-01-01

    -municipality variation in caries experience. Materials and methods Data was collected in the public Child Dental Health Service. In total, 5636 caries registrations on 3-, 9-, 15- and 18-year-olds were collected in 35 of the 98 Danish municipalities. Caries experience was expressed by mean def-s/DMF-S and caries......Objective The aim of this study was to describe the caries experience, prevalence and distribution related to tooth type and surfaces in the primary and permanent dentition in children and adolescents in Denmark in 2012. In addition, to examine if explanatory factors influence the inter...... prevalence by def-s = 0/DMF-S = 0. Inter-municipality variations were illustrated. Multivariate regression analyses were applied to assess the influence of fluoride concentration in drinking water, proportion of immigrants and personal income on the inter-municipality variation in mean def-s/DMF-S. Results...

  19. Use of recombinant adenovirus vectored consensus IFN-α to avert severe arenavirus infection.

    Directory of Open Access Journals (Sweden)

    Brian B Gowen

    Full Text Available Several arenaviruses can cause viral hemorrhagic fever, a severe disease with case-fatality rates in hospitalized individuals ranging from 15-30%. Because of limited prophylaxis and treatment options, new medical countermeasures are needed for these viruses classified by the National Institutes of Allergy and Infectious Diseases (NIAID as top priority biodefense Category A pathogens. Recombinant consensus interferon alpha (cIFN-α is a licensed protein with broad clinical appeal. However, while cIFN-α has great therapeutic value, its utility for biodefense applications is hindered by its short in vivo half-life, mode and frequency of administration, and costly production. To address these limitations, we describe the use of DEF201, a replication-deficient adenovirus vector that drives the expression of cIFN-α, for pre- and post-exposure prophylaxis of acute arenaviral infection modeled in hamsters. Intranasal administration of DEF201 24 h prior to challenge with Pichindé virus (PICV was highly effective at protecting animals from mortality and preventing viral replication and liver-associated disease. A significant protective effect was still observed with a single dosing of DEF201 given two weeks prior to PICV challenge. DEF201 was also efficacious when administered as a treatment 24 to 48 h post-virus exposure. The protective effect of DEF201 was largely attributed to the expression of cIFN-α, as dosing with a control empty vector adenovirus did not protect hamsters from lethal PICV challenge. Effective countermeasures that are highly stable, easily administered, and elicit long lasting protective immunity are much needed for arena and other viral infections. The DEF201 technology has the potential to address all of these issues and may serve as a broad-spectrum antiviral to enhance host defense against a number of viral pathogens.

  20. TU-F-CAMPUS-T-05: Dose Escalation to Biological Tumor Volumes of Prostate Cancer Patients Using Gold Nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Jermoumi, M; Ngwa, W [Department of Physics and Applied Physics, Medical Physics Program, University of Massachusetts Lowell (United States); Department of Radiation Oncology, Dana Farber Cancer Insitute, Brigham and Women’s Hospital, Harvard Medical, Boston, MA (United States); Sajo, E [Department of Physics and Applied Physics, Medical Physics Program, University of Massachusetts Lowell (United States); Houari, K [Department of Radiation Oncology, Dana Farber Cancer Insitute, Brigham and Women’s Hospital, Harvard Medical, Boston, MA (United States)

    2015-06-15

    Purpose: Studies have shown that radiation boosting could help reduce prostate cancer (PCa) recurrence. Biological tumor volumes (BTV) are a high priority for such radiation boosting. The purpose of this study is to investigate the potential of radiation boosting of real patient BTVs using gold nanoparticles (GNP) released from gold-loaded brachytherapy spacers (GBS) during brachytherapy. Methods: The BTVs of 12 patients having prostate adenocarcinoma identified with positron emission tomography (PET) and CT scanner using C-11 labeled tracer [11C]acetate were investigated. The initial GNP concentration and time to achieve a dose enhancement effect (DEF) of 2 was simulated using the freely downloadable software RAID APP. The investigations were carried out for low dose rate (LDR) brachytherapy sources (BTS) described in AAPM Task Group report 43: Cs-131, I-125, and Pd-103. In first case, we used 7 mg/g and 18 mg/g of GNP initial concentrations to estimate the time needed for released GNP to achieve a DEF of 2 for the different BTS, and compare with clinically relevant treatment times. In second case, we calculated the initial concentration of GNPs needed to achieve a DEF of 2 during the time the BTS would typically deliver 50%, 70% and 90% of the total dose. Results: For an initial concentration of 18 mg/g, when using Cs-131, and Pd-103, a DEF of 2 could only be achieved for BTV of 3.3 cm3 and 1 cm3 respectively. Meanwhile a DEF of 2 could be achieved for all 12 BTVs when using I-125. To achieve a DEF of 2 for all patients using Cs-131 and Pd-103, much higher initial concentrations would have to be used than have been typically employed in pre-clinical studies. Conclusion: The I-125 is the most viable BTS that can be employed with GBS to guide dose painting treatment planning for localized PCa.

  1. SU-E-T-333: Towards Customizable Radiotherapy Enhancement (CuRE) for Prostate Cancer Using Cisplatin Nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Sinha, N [Wentworth Institute of Technology, Boston, MA (United States); Cifter, G [University of Massachusetts Lowell, Dana Farber Cancer Institute, Lowell, MA (United States); Sajo, E [UniversityMassachusetts Lowell, Lowell, MA (United States); Ngwa, W [Harvard Medical School, Boston, MA (United States)

    2014-06-01

    Purpose: Replacing routinely used brachytherapy spacers with multifunctional ones loaded with cisplatin nanoparticles (CNP), which can be released into the tumor after implantation, could enable customizable radiation boosting to the prostate tumor in addition to chemotherapy effect. This study investigates the feasibility of customizing the intra-tumor biodistribution and corresponding dose enhancement (DEF) over time for the released CNP as a function of nanoparticle size. Methods: Dose enhancement factors (DEF) due to photon-induced emission of photo-/Auger electrons from CNPs were calculated as a function of concentration using previously published analytical calculation method. An experimentally determined diffusion coefficient (D) for 10 nm nanoparticles in mouse tumor model was employed to estimate D for other sizes using the Stoke- Einstein equation. The error function diffusion model in the experimental study was applied to generate the intra-tumor concentration profile for a burst release of CNPs from the spacer over time. The corresponding DEF profiles were then determined for brachytherapy using Pd-103 and I-125 sources. Results: As expected, the generated profiles showed greater DEF over time for smaller CNP sizes at sample distances from the spacer. For example, for a centrally located spacer, clinically significant DEF (> 20%) could be achieved near the tumor periphery (ca. 0.85 cm distance from the spacer for average PCa tumor size) after 20, and 100 days, respectively for CNPs sizes of 2 nm, and 10 nm, using I-125. Meanwhile for Pd-103, clinically significant DEF could be achieved at the same position after 22 and 108 days, respectively, for same size particles. Conclusion: Our preliminary results demonstrate the feasibility of customizing dose enhancement to prostate tumors as a function of spacer location, brachytherapy source type or size of CNPs released from multifunctional spacers. Such an approach could enable customizable radiation boosting

  2. The role of whole-wheat grain and wheat and rye ingredients on the digestion and fermentation processes in the gut - a model experiment with pigs

    DEFF Research Database (Denmark)

    Legall, Maud; Serena, Anja; Jørgensen, Henry

    2009-01-01

    fibre (DF) but differed in arabinoxylan (AX) content and composition. Twenty pigs were fed the breads three times daily (08.00, 13.00 and 18.00 hours) and the digesta collected through a T-cannula for two successive periods (breakfast: 8.00-13.00; lunch: 13.00-18.00 hours). Faeces were collected for 24...

  3. Photosynthetic parameters and growth in seedlings of Bertholletia excelsa and Carapa guianensis in response to pre-acclimation to full sunlight and mild water stress

    National Research Council Canada - National Science Library

    Azevedo, Gracilene Fernandes da Costa

    2014-01-01

    .... I used six independent pre-acclimation treatments (0, 90 (11h15-12h45), 180 (10h30-13h30), 360 (09h00-15h00), 540 (07h30-16h30) and 720 min (06h00-18h00)) varying the time of exposure to full sunlight...

  4. JVLA detection of H1743-322 in its hard state

    Science.gov (United States)

    Mooley, K. P.; Tendulkar, S. P.; Walton, D. J.; Fuerst, F.; Harrison, F. A.; Tomsick, J. A.

    2014-09-01

    We carried out radio observations of the blackhole X-ray binary H1743-322 (IGR J17464-3213, ATel#6474) with the Jansky VLA in the X-band (8-12 GHz). The on-source observations were carried out between 2014 Sep 20, 23:56:26 UT and 2014 Sep 21, 00:18:22 UT.

  5. 77 FR 75014 - Schedule of Fees for Access to NOAA Environmental Data, Information, and Related Products and...

    Science.gov (United States)

    2012-12-19

    ... products is growing rapidly, both in size and sophistication, and as a result the associated costs have... In-Situ Digital Data......... 6.00 (*) Data Poster 18.00 18.00 Shipping Service for orders totaling... unit size is 1.2 gigabytes) Offline Radar Digital Data (Level II) (average unit size is 4...

  6. 77 FR 58973 - Airworthiness Directives; Eurocopter France Helicopters

    Science.gov (United States)

    2012-09-25

    ... helicopter nose to drop and hit the ground while the rotor blades are spinning. DATES: We must receive... designs. Related Service Information We reviewed Eurocopter ASB No. 32.00.18, Revision 2, dated July 12... the FAA with promoting safe flight of civil aircraft in air commerce by prescribing regulations for...

  7. 北京夜生活(英文)

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Galleries画廊CHAOYANG DISTRICTChina Art Archives & WarehouseTel:84565152Add:East of Tiedao Bridge,CaochangdianVillageHours:Tuesday-Sunday,13:00-18:00Creation GalleryTel:85617570Add:North entrance of Ritan East RoadNortheast corner of Ritan Park

  8. Air University Library Index to Military Periodicals. Volume 42, Number 3, July-September 1991

    Science.gov (United States)

    1991-09-01

    Administration. See issues of AMMON, Steve KE ASAT (kinetic energy anti-satellite). Armed Forces J Intl -- and Plato, Gus Russell Lange , Maj and Sean B. (SecDef...A R. See COMBAT SUPPORT OF Serge M. Duiflinger. illus bibliog. CARLSON, Craig L, Maj THE ARMY Canadian Def Q 20 no 6 (Summer/Jun Measures of...PILOTED VEHICLES Brief 43 no 4 (Jul-Aug 󈨟): p13. Chart no 3 (Aug 26 󈨟): p6 DRUG ENFORCEMENT only EDUCATION, MILITARY ADMINISTRATION DURFLINGER, Serge M

  9. Design Document and Database Specifications for the Naval Interactive Data Analysis System (NIDAS) Version 1.0

    Science.gov (United States)

    1993-10-29

    min-jext; Widget step- Sext ; Widget colorj-ext; Pixmnap pixmap; Boolean if~erelsPixmaP; double * Ion; double *lat; float ininVal; float maxVal; float...struct { Widget lvIljist; Widget parmjlist; Widget mnonthjIist; Widget min-..text; Widget max-text; Widget step-jext; Widget color.. Sext ; Pixmap pixmnap...0 ~~ 5 __ int df~aylolo0 int defDay21Color,4 int defDay2Color. Wigt day Iy3color-tx; Widget dayl color..jext; Widget day3-color.. sext ; int dayl1; int

  10. On the Blow-up Criterion of Smooth Solutions to the MHD System in BMO Space

    Institute of Scientific and Technical Information of China (English)

    Bao-quan Yuan

    2006-01-01

    In this paper we study the blow-up criterion of smooth solutions to the incompressible magnetohydrodynamics system in BMO space. Let (u(x,t),b(x,t)) be smooth solutions in (0, T). It is shown that the solution (u(x, t), b(x, t)) can be extended beyond t = T if (u(x, t), b(x, t)) ∈ L1(0, T; BMO) or the vorticity(rot u(x, t), rot b(x, t)) ∈ L1 (0, T; BMO) or the deformation (Def u(x, t), Def b(x, t)) ∈ L1 (0, T; BMO).

  11. Methods for Identifying Part Quality Issues and Estimating Their Cost with an Application Using the UH-60

    Science.gov (United States)

    2014-01-01

    WPU report. TOP 5 Items with PQDRs by Value ($M) RCN# (*Oldest PQDR) Nomenclature I/E SOS NIIN CAT Qty Def Unit Cost Total # PQDR s End Item Value...401667 4 AH-64D 1.581 145 TOP 5 PQDRs by Age (Days) RCN# Nomenclature I/E SOS NIIN CAT Qty Def Unit Cost End Item Value ($M) Age (Days) W45N7V-09-0274...customer dissatisfaction leading to lost sales (and revenue), • compromised employee morale due to persistent quality issues, • increased scrap and

  12. Omega-3 fatty acid deficiency does not alter the effects of chronic fluoxetine treatment on central serotonin turnover or behavior in the forced swim test in female rats.

    Science.gov (United States)

    McNamara, Robert K; Able, Jessica A; Liu, Yanhong; Jandacek, Ronald; Rider, Therese; Tso, Patrick; Lipton, Jack W

    2013-12-01

    While translational evidence suggests that long-chain omega-3 fatty acid status is positively associated with the efficacy of selective serotonin reuptake inhibitor drugs, the neurochemical mechanisms mediating this interaction are not known. Here, we investigated the effects of dietary omega-3 (n-3) fatty acid insufficiency on the neurochemical and behavioral effects of chronic fluoxetine (FLX) treatment. Female rats were fed diets with (CON, n=56) or without (DEF, n=40) the n-3 fatty acids during peri-adolescent development (P21-P90), and one half of each group was administered FLX (10mg/kg/day) for 30days (P60-P90) prior to testing. In adulthood (P90), regional brain serotonin (5-HT) and 5-hydroxyindoleacetic (5-HIAA) concentrations, presynaptic markers of 5-HT neurotransmission, behavioral responses in the forced swim test (FST), and plasma FLX and norfluoxetine (NFLX) concentrations were investigated. Peri-adolescent n-3 insufficiency led to significant reductions in cortical docosahexaenoic acid (DHA, 22:6n-3) composition in DEF (-25%, p≤0.0001) and DEF+FLX (-28%, p≤0.0001) rats. Untreated DEF rats exhibited significantly lower regional 5-HIAA/5-HT ratios compared with untreated CON rats, but exhibited similar behavioral responses in the FST. In both CON and DEF rats, chronic FLX treatment similarly and significantly decreased 5-HIAA concentrations and the 5-HIAA/5-HT ratio in the hypothalamus, hippocampus, and nucleus accumbens, brainstem tryptophan hydroxylase-2 mRNA expression, and immobility in the FST. While the FLX-induced reduction in 5-HIAA concentrations in the prefrontal cortex was significantly blunted in DEF rats, the reduction in the 5-HIAA/5-HT ratio was similar to CON rats. Although plasma FLX and NFLX levels were not significantly different in DEF and CON rats, the NFLX/FLX ratio was significantly lower in DEF+FLX rats. These preclinical data demonstrate that n-3 fatty acid deficiency does not significantly reduce the effects of chronic

  13. CLONING AND SEQUENCING OF THE DEFENSIN A GENE FROM AEDES AEGYPTI AND AE.ALBOPICTUS%白纹伊蚊和埃及伊蚊defensin A基因克隆及序列分析

    Institute of Scientific and Technical Information of China (English)

    刘先凯; 赵彤言; 朱礼华; 董言德; 陆宝麟

    2002-01-01

    应用PCR技术从白纹伊蚊和埃及伊蚊基因组中扩增出defensin A基因,并与文献报道的defensin A的5个型的cDNA序列进行同源性比较,发现此两序列中存在内元;从埃及伊蚊体内扩增的片段为蚊虫defensin Al的前体AaDef Al;从白纹伊蚊体内扩增的片段为defensin A的1个新型,命名为DefA6.

  14. Deschutes estuary feasibility study: hydrodynamics and sediment transport modeling

    Science.gov (United States)

    George, Douglas A.; Gelfenbaum, Guy; Lesser, Giles; Stevens, Andrew W.

    2006-01-01

    Continual sediment accumulation in Capitol Lake since the damming of the Deschutes River in 1951 has altered the initial morphology of the basin. As part of the Deschutes River Estuary Feasibility Study (DEFS), the United States Geological Survey (USGS) was tasked to model how tidal and storm processes will influence the river, lake and lower Budd Inlet should estuary restoration occur. Understanding these mechanisms will assist in developing a scientifically sound assessment on the feasibility of restoring the estuary. The goals of the DEFS are as follows. - Increase understanding of the estuary alternative to the same level as managing the lake environment.

  15. Arctic Collaboration: Developing a Successful Researcher/Teacher Expedition

    Science.gov (United States)

    Skotnicki, S.; Loranty, M. M.

    2016-12-01

    Are you a researcher working in the polar regions of the world or a K-12 science teacher who would like to be part of a field research expedition in the polar regions? Researchers and K-12 science teachers can apply for funding from PolarTREC, a program that pairs researchers and teachers to conduct field science in Antarctica and the Arctic. Our poster presentation will offer details of one such successful researcher/teacher partnership. During the summer of 2016, Science Teacher Stan Skotnicki (Cheektowaga Central Middle School in Buffalo, NY) was teamed up with Assistant Professor Mike Loranty (Colgate University) to study vegetation and ecosystem impacts on permafrost vulnerability. Stan joined Mike and his research team in Northeastern Siberia preparing field sites, collecting data, processing samples, discussing methods, and planning daily activities. In order to raise awareness and broaden the impact of the research being conducted, Stan communicated the science through a series of journals on the PolarTREC website with his students, staff, and members of the community. Additionally, Mike and Stan held a live webinar from Siberia discussing the content of the research, the nature of the fieldwork, and why it was important to travel so far for this information. This expedition allowed Stan to experience working with a field research team for an extended period of time. Mike benefited from having a team member dedicated to learning about and communicating project details that also provided valuable field assistance. Stan gets to bring his hands-on experience back to his classroom in Buffalo and Mike has the opportunity to share his research with a new and different audience, including presenting to students at Cheektowaga Central with the help of his undergraduate students. This model of collaboration provides a number of valuable benefits for both teachers and researchers. While the PolarTREC program provides necessary logistics and funding to conduct these

  16. 食用植物油的脂肪酸组成调查

    Institute of Scientific and Technical Information of China (English)

    张晓波

    2005-01-01

    针对当前市场销售的23个品牌9种小包装食用植物油的脂肪酸组成进行气相色谱分析结果表明,10个品牌大豆油和大豆色拉油、4个品牌花生油、2个品牌葵花油和玉米油、8个品牌的芝麻油、1个品牌山茶油和1个品牌橄榄油,均符合新颁布的五种食用植物油国家标准和CODEX STAN 210-1999、CODEX STAN33的要求.

  17. Health-related Quality of Life after complex rectal surgery for primary advanced rectal cancer and locally recurrent rectal cancer

    DEFF Research Database (Denmark)

    Thaysen, Henriette Vind

    2013-01-01

    L after treatment with COMP-RCS. Seven studies fulfilled the inclusion criteria. Different aspects of HRQoL seemed to be impaired for a shorter or longer period of time after surgery, in disease free patients treated for PARC and LRRC. However, the included studies all had methodological problems, which...... in the study was 164 (86%) patients treated with standard rectal cancer surgery (STAN-RCS). The Danish version showed satisfactory psychometric properties for the scales concerning body image, sexual functioning, male sexual problems and defecations problems. Reduced psychometric properties were found....... The majority of the scales improved or remained stable during the first year after surgery, a decrease was seen only for body image. One year after surgery HRQoL in patients with COMP-RSC was comparable to patients with STAN-RCS and NORM-data with exception of a poorer physical and emotional role function...

  18. ATLAS TileCal Sub-Module Production at UIUC - Production Submodule Photos (current)

    CERN Multimedia

    Errede, Steve

    2000-01-01

    Step 5 - Welding tacl_weld - Our Welder from Nucor (Tuscola, IL) tack-welds each of the four corners of the submodule. The submodule can then be safely lifted out of the stacking fixture by crane. Step 5 - WELDING tack_weld - Stan Lamb, our welder from Nucor (Tuscola, IL) tack-welds each of the four corners of the submodule. The submodule can then be safely lifted out of the stacking fixture by crane. Photo 9 - This is our handy Submodule Final Welding Jig designed and built by our very own Fred Cogswell. Photo 10 - A submodule being lowered into the welding jig for final welding. Photo 11 - Stan Lamb final-welds the submodule. The welding jig saves us an enormous amount of time, since the submodule can be easily be rotated by hand for each weld.

  19. Antimicrobial and cytotoxic effects of Mexican medicinal plants.

    Science.gov (United States)

    Jacobo-Salcedo, Maria del Rosario; Alonso-Castro, Angel Josabad; Salazar-Olivo, Luis A; Carranza-Alvarez, Candy; González-Espíndola, Luis Angel; Domínguez, Fabiola; Maciel-Torres, Sandra Patricia; García-Lujan, Concepción; González-Martínez, Marisela del Rocio; Gómez-Sánchez, Maricela; Estrada-Castillón, Eduardo; Zapata-Bustos, Rocio; Medellin-Milán, Pedro; García-Carrancá, Alejandro

    2011-12-01

    The antimicrobial effects of the Mexican medicinal plants Guazuma ulmifolia, Justicia spicigera, Opuntia joconostle, O. leucotricha, Parkinsonia aculeata, Phoradendron longifolium, P. serotinum, Psittacanthus calyculatus, Tecoma stans and Teucrium cubense were tested against several human multi-drug resistant pathogens, including three Gram (+) and five Gram (-) bacterial species and three fungal species using the disk-diffusion assay. The cytotoxicity of plant extracts on human cancer cell lines and human normal non-cancerous cells was also evaluated using the MTT assay. Phoradendron longifolium, Teucrium cubense, Opuntia joconostle, Tecoma stans and Guazuma ulmifolia showed potent antimicrobial effects against at least one multidrug-resistant microorganism (inhibition zone > 15 mm). Only Justicia spicigera and Phoradendron serotinum extracts exerted active cytotoxic effects on human breast cancer cells (IC50 plant species may be important sources of antimicrobial and cytotoxic agents.

  20. Na charisma komt soberheid: charisma en leiderschap

    NARCIS (Netherlands)

    L. Karsten; S. Keulen; R. Kroeze

    2008-01-01

    Waarom worden charismatische leiders, zowel in politiek als bedrijfsleven, historisch gezien altijd opgevolgd door introvertere types? "Een charismatische leider put een organisatie uit", stellen de auteurs van dit artikel. "Maar er is geen enkele reden om aan te nemen dat charismatische leiders def

  1. EURECCA colorectal : Multidisciplinary Mission statement on better care for patients with colon and rectal cancer in Europe

    NARCIS (Netherlands)

    van de Velde, Cornelis J. H.; Aristei, Cynthia; Boelens, Petra G.; Beets-Tan, Regina G. H.; Blomqvist, Lennart; Borras, Josep M.; van den Broek, Colette B. M.; Brown, Gina; Coebergh, Jan-Willem; Van Cutsem, Eric; Espin, Eloy; Gore-Booth, Jola; Glimelius, Bengt; Haustermans, Karin; Henning, Geoffrey; Iversen, Lene H.; van Krieken, J. Han; Marijnen, Corrie A. M.; Mroczkowski, Pawel; Nagtegaal, Iris; Naredi, Peter; Ortiz, Hector; Pahlman, Lars; Quirke, Philip; Roedel, Claus; Roth, Arnaud; Rutten, Harm J. T.; Schmoll, Hans J.; Smith, Jason; Tanis, Pieter J.; Taylor, Claire; Wibe, Arne; Gambacorta, Maria Antonietta; Meldolesi, Elisa; Wiggers, Theo; Cervantes, Andres; Valentini, Vincenzo

    2013-01-01

    Background: Care for patients with colon and rectal cancer has improved in the last twenty years however still considerable variation exists in cancer management and outcome between European countries. Therefore, EURECCA, which is the acronym of European Registration of cancer care, is aiming at def

  2. 26 CFR 1.414(c)-3 - Exclusion of certain interests or stock in determining control.

    Science.gov (United States)

    2010-04-01

    ... Plans, Etc. § 1.414(c)-3 Exclusion of certain interests or stock in determining control. (a) In general... common control. Thus, DEF Partnership is considered to own stock possessing 88.2 percent (75÷85) of the... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Exclusion of certain interests or stock in...

  3. Entrepreneurial Orientation : Multilevel analysis and Consequences

    NARCIS (Netherlands)

    Rigtering, J.P.C.

    2013-01-01

    Entrepreneurial orientation (EO) has become one of the most important foci within the domain of entrepreneurship research. EO is distinguished from entrepreneurship, which is generally studied in relation to new entry, by its focus on the processes, practices, and decision-making activities that def

  4. A program to solve rotating plasma problems

    NARCIS (Netherlands)

    Bakker, M.; Berg, M.S. van den

    1980-01-01

    It is shown that the solution of a rotating plasma problem minimizes a quitably chosen funtional. This variational problem is solved by the Ritz-Galerkin methud using piecewise bilinear functions and applying some Newton-Côtes-like quadrature. The resulting linear system with a sparse nonegative def

  5. Interdisciplinary Studies on the Combat Readiness and Health Issues Faced by Military Personnel. Addendum

    Science.gov (United States)

    2010-09-01

    horses , and birds can be formed and differentiated from one another based on perceptual features (French et al., 2004). In fact, it has been found that...deficits in disorders such as Attention Def- icit Hyperactivity Disorder, dyslexia, autism , and degenerative dis- eases will likely necessitate tasks that

  6. EQUIVALENT MODELS IN COVARIANCE STRUCTURE-ANALYSIS

    NARCIS (Netherlands)

    LUIJBEN, TCW

    1991-01-01

    Defining equivalent models as those that reproduce the same set of covariance matrices, necessary and sufficient conditions are stated for the local equivalence of two expanded identified models M1 and M2 when fitting the more restricted model M0. Assuming several regularity conditions, the rank def

  7. Association studies of the copy-number variable ß-defensin cluster on 8p23.1 in adenocarcinoma and chronic pancreatitis

    Directory of Open Access Journals (Sweden)

    Taudien Stefan

    2012-11-01

    Full Text Available Abstract Background Human ß-defensins are a family of antimicrobial peptides located at the mucosal surface. Both sequence multi-site variations (MSV and copy-number variants (CNV of the defensin-encoding genes are associated with increased risk for various diseases, including cancer and inflammatory conditions such as psoriasis and acute pancreatitis. In a case–control study, we investigated the association between MSV in DEFB104 as well as defensin gene (DEF cluster copy number (CN, and pancreatic ductal adenocarcinoma (PDAC and chronic pancreatitis (CP. Results Two groups of PDAC (N=70 and CP (N=60 patients were compared to matched healthy control groups CARLA1 (N=232 and CARLA2 (N=160, respectively. Four DEFB104 MSV were haplotyped by PCR, cloning and sequencing. DEF cluster CN was determined by multiplex ligation-dependent probe amplification. Neither the PDAC nor the CP cohorts show significant differences in the DEFB104 haplotype distribution compared to the respective control groups CARLA1 and CARLA2, respectively. The diploid DEF cluster CN exhibit a significantly different distribution between PDAC and CARLA1 (Fisher’s exact test P=0.027, but not between CP and CARLA2 (P=0.867. Conclusion Different DEF cluster b CN distribution between PDAC patients and healthy controls indicate a potential protective effect of higher CNs against the disease.

  8. EXTENT AND LOCATION OF INTRARETINAL AND SUBRETINAL FLUID AS PROGNOSTIC FACTORS FOR THE OUTCOME OF PATIENTS WITH OPTIC DISK PIT MACULOPATHY

    NARCIS (Netherlands)

    Steel, D.H.; Williamson, T.H.; Laidlaw, D.A.; Sharma, P.; Matthews, C.; Rees, J.; Petrou, P.; Charteris, D.G.; Charles, S.J.; Patel, C.K.; Crama, N.; Herbert, E.; Jackson, T.L.

    2016-01-01

    PURPOSE: To determine if baseline fundoscopic and optical coherence tomography (OCT) features influence the clinical course of optic disk pit maculopathy. METHODS: A multicenter retrospective case note review was undertaken, using standardized OCT and clinical data collection. Visual success was def

  9. [Kinetics of serum albumin adsorption on the macroporous glass MPS-250 GKH].

    Science.gov (United States)

    Naumova, L V; El'Kin, G E; Dmitrenko, L V

    1996-01-01

    Intrinsic diffusion (defined as diffusion within micropores or microgranules) was shown to be a major factor that determines the kinetics of bovine serum albumin adsorption to macroporous silica MPS-250 GKh. The effective coefficient of intrinsic diffusion (within the silica phase) was calculated (Def = 7 x 10(-7) cm2/s).

  10. Maternal Intake of Fish Oil but not of Linseed Oil Reduces the Antibody Response in Neonatal Mice

    DEFF Research Database (Denmark)

    Lauritzen, Lotte; Kjær, T. M. R.; Porsgaard, Trine

    2011-01-01

    . From the day of conception and throughout lactation, dams were fed diets containing 4% fat from linseed oil (LSO), fish oil (FO) or a n-3 PUFA-deficient diet (DEF). Pups were injected with OVA within 24 h of birth and sacrificed at weaning (day 21). Overall, the content of n-3 PUFA in milk, liver...

  11. Knockout of an outer membrane protein operon of anaplasma marginale by transposon mutagenesis

    Science.gov (United States)

    Large amounts of data generated by genomics, transcriptomics and proteomics technologies have increased our understanding of the biology of Anaplasma marginale. However, these data have also led to new assumptions that require testing, ideally through classic genetic mutation. One example is the def...

  12. InterProScan Result: FY738277 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY738277 FY738277_1_ORF1 DEF344C33DA7903A PFAM PF00067 p450 1e-23 T IPR001128 Cytochrome P450 Molecular... Function: monooxygenase activity (GO:0004497)|Molecular Function: iron ion binding (GO:0005506)|Molecular... Function: electron carrier activity (GO:0009055)|Molecular Function: heme binding (GO:0020037) ...

  13. MCTRSS Overview Functional Description

    Science.gov (United States)

    1994-05-16

    PUB 184 Integration Definition for Infonnafi• Modeling ( IDEFIX ) 21 December 1993 Training and Education Division, MCCDC, Training Readiness Needs...directives 8320.l-M, 8320.1-M-1, 8320-M-x, and FIPS PUB 184. 2 IDEFIX . IDEF is an acronym for ICAM DEF’nition, where ICAM stands for Integrated

  14. Impaired sense of smell and color discrimination in monogenic and idiopathic Parkinson's disease

    DEFF Research Database (Denmark)

    Kertelge, Lena; Brüggemann, Norbert; Schmidt, Alexander

    2010-01-01

    Olfaction is typically impaired in idiopathic Parkinson's disease (IPD), but its role is uncertain in monogenic PD. Diminished color discrimination has been suggested as another early sign of dopaminergic dysfunction but not been systematically studied. Furthermore, it is unknown whether both def...

  15. 12'' : Nõela all / Ahto Vahter

    Index Scriptorium Estoniae

    Vahter, Ahto

    1998-01-01

    Uutest plaatidest Krust "True Stories", Urban Species "Woman", The Interloper "Get Together", Mos Def & Talib Kweli "Black Star", The Brian Setzer Orchestra "The Dirty Boogie", OST "The Avengers", Jhelisa "Friendly Pressure", Ordinary People feat.Jude Mansi "The Missing", More So feat. Filthy Rich & Damon Trueitt "Take My Hand", Amira "My Desire"

  16. An Application of the Job Characteristics Model to Enlisted Strategic Air Command Missile Maintenance Career Fields.

    Science.gov (United States)

    1985-09-01

    travel to and from Launch Facilities and Launch Control Facilities. Then pile on all the extra classes that field runners are required to be at during off...Principles of Management and Organizational Behavior (Fourth Edition). Columbus OH: Charles E. Merrill Publishing Company, 1977. 29. Maze , Rick. "SecDef: 3

  17. Short-term high temperature growth conditions during vegetative-to-reproductive phase transition irreversibly compromise cell wall invertase-mediated sucrose catalysis and microspore meiosis in grain sorghum

    Science.gov (United States)

    Grain sorghum (Sorghum bicolor L. Moench) crop yield is significantly compromised by high temperature stress-induced male sterility, and is attributed to reduced cell wall invertase (CWI)-mediated sucrose hydrolysis in microspores and anthers leading to altered carbohydrate metabolism and starch def...

  18. Effects of the synergists piperonyl butoxide and S,S,S-tributyl phosphorotrithioate on propoxur pharmacokinetics in Blattella germanica (Blattodea: Blattellidae).

    Science.gov (United States)

    Sanchez-Arroyo, H; Koehler, P G; Valles, S M

    2001-10-01

    Effects of the synergists piperonyl butoxide (PBO) and S,S,S-tributyl phosphorotrithioate (DEF) on propoxur pharmacokinetics were examined in the German cockroach, Blattella germanica (L.). Treatment of adult male German cockroaches with the cytochrome P450 monooxygenase inhibitor, PBO, or the esterase inhibitor, DEF, increased propoxur toxicity by 2- and 6.8-fold, respectively, implicating hydrolysis as a major detoxification route of propoxur in the German cockroach. However, significant hydrolytic metabolism could not be demonstrated conclusively in vitro resulting in a conflict between in situ bioassay data and in vitro metabolic studies. In vitro propoxur metabolism with NADPH-fortified microsomes produced at least nine metabolites. Formation of metabolites was NADPH-dependent; no quantifiable metabolism was detected with cytosolic fractions. However, microsomal fractions lacking an NADPH source did produce a low, but detectable, quantity of metabolites (1.6 pmol). PBO inhibited NADPH-dependent propoxur metabolism in a dose-dependent fashion, implicating cytochrome P450 monooxygenases as the enzyme system responsible for the metabolism. Interestingly, DEF also inhibited the NADPH-dependent metabolism of propoxur, albeit to a lower extent. Treatment with PBO or DEF also caused a significant reduction in the cuticular penetration rate of propoxur. The data demonstrate that unanticipated effects are possible with synergists and that caution must be exercised when interpreting synergist results.

  19. Full charge-density scheme with a kinetic-energy correction: Application to ground-state properties of the 4d metals

    DEFF Research Database (Denmark)

    Vitos, Levente; Kollár, J.; Skriver, Hans Lomholt

    1997-01-01

    We present a full charge-density technique to evaluate total energies from the output of self-consistent linear muffin-tin orbitals (LMTO) calculations in the atomic-sphere approximation (ASA). The Coulomb energy is calculated exactly from the complete, nonspherically symmetric charge density def...

  20. 76 FR 32886 - Control of Emissions From New Highway Vehicles and Engines; Guidance on EPA's Certification...

    Science.gov (United States)

    2011-06-07

    ... driver behavior. They concluded that a 25 percent engine torque derate would not induce a corrective... intended effect of motivating appropriate driver behavior. The report of Navistar's study found that some... technologies require drivers to refill DEF on a regular basis and are dependent on appropriately...

  1. Symmetries, conservation laws, and time reversibility for Hamiltonian systems with external forces

    NARCIS (Netherlands)

    Schaft, A.J. van der

    1983-01-01

    A system theoretic framework is given for the description of Hamiltonian systems with external forces and partial observations of the state. It is shown how symmetries and conservation laws can be defined within this framework. A generalization of Noether's theorem is obtained. Finally a precise def

  2. Product-oriented environmental management : a study of capability-building, stakeholder orientation and continuous improvement regarding products' environmental characteristics in firms

    NARCIS (Netherlands)

    Bakker, de Frank G.A.

    2001-01-01

    Companies are increasingly giving attention to the environmental characteristics of their products. This book focuses on the ways in which large, proactive, firms could develop and maintain product-oriented environmental management (POEM), both at the strategic and the operational level. POEM is def

  3. Cold gas properties of the Herschel Reference Survey. III. Molecular gas stripping in cluster galaxies

    CERN Document Server

    Boselli, A; Boquien, M; Boissier, S; Catinella, B; Gavazzi, G; Lagos, C; Saintonge, A

    2014-01-01

    The HRS is a complete volume-limited sample of nearby objects including Virgo cluster and isolated objects. Using a recent compilation of HI and CO data we study the effects of the cluster on the molecular gas content of spiral galaxies. We first identify M* as the scaling variable that traces the total H2 mass of galaxies better. We show that, on average, HI-deficient galaxies are significantly offset from the M(H2) vs. M* relation for HI-normal galaxies. We use the M(H2) vs. M* scaling relation to define the H2-deficiency parameter. This parameter shows a weak and scattered relation with the HI-def, here taken as a proxy for galaxy interactions with the cluster environment. We also show that, as for the HI, the extent of the H2 disc decreases with increasing HI-deficiency. These results show that cluster galaxies have, on average, a lower H2 content than similar objects in the field. The slope of the H2-def vs. HI-def relation is less than 1, while the D(HI)/D(i) vs. HI-def relation is steeper than the D(CO...

  4. Impact of straw and rock-fragment mulches on soil moisture and early growth of holm oaks in a semiarid area

    Science.gov (United States)

    M. N. Jimenez; J. R. Pinto; M. A. Ripoll; A. Sanchez-Miranda; F. B. Navarro

    2017-01-01

    Planted seedlings and saplings usually exhibit low survival and growth rates under dry Mediterranean environments, especially late-successional species such as Quercus. In this work, we studied the effects of straw and rock fragment mulches on the establishment conditions of holm oak (Quercus ilex L. subsp. ballota (Def.) Samp.) in SE Spain. Soil moisture was...

  5. Dicty_cDB: Contig-U14871-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 956( CP000082 |pid:none) Psychrobacter arcticus 273-4, co... 101 3e-20 CP001277_1859( CP001277 |pid:none) Candidatus Hamiltonella def...ensa... 101 3e-20 (A4IYN5) RecName: Full=Ribose-5-phosphate isomerase A; ... 101 3e

  6. Impaired biosynthesis of the non-bilayer lipids phosphatidylethanolamine or cardiolipin does not affect peroxisome biogenesis and proliferation in Saccharomyces cerevisiae

    NARCIS (Netherlands)

    Kawałek, Adam; Jagadeesan, Chandhuru; van der Klei, Ida J

    2016-01-01

    The non-bilayer forming lipids cardiolipin (CL) and phosphatidylethanolamine (PE) modulate membrane curvature, facilitate membrane fusion and affect the stability and function of membrane proteins. Yeast peroxisomal membranes contain significant amounts of CL and PE. We analysed the effect of CL def

  7. Dicty_cDB: Contig-U14415-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1_119( CP001281 |pid:none) Thauera sp. MZ1T, complete genome. 43 0.015 CP001277_1694( CP001277 |pid:none) Candidatus Hamiltonella def...ensa... 42 0.020 CT573326_3376( CT573326 |pid:none) Pseudomonas entomophila str. L4

  8. Pop / Tristan Priimägi

    Index Scriptorium Estoniae

    Priimägi, Tristan, 1976-

    2008-01-01

    Heliplaatidest: Elbow "The Seldom Seen Kid", Lahesõda "Dermocracy", Nits "Doing The Dishes", Nine Inch Nails "Ghosts I-IV", The Last Shadow Puppets "The Age Of The Understatement", The Shortwave Set "Replica Sun Machine", Def Leppard "Songs From The Sparkle Lounge"

  9. Definiciones conceptual y operacional de masa molecular

    OpenAIRE

    Varón Castellanos, Ramón; Martín Monteagudo, Isidoro; González Roldán, Ismael

    1986-01-01

    En este trabajo se da una defínición estadística de masa molecular y se relaciona con la definición operacional dada normalmente en la literatura, demostrando que la segunda es una consecuencia de la primera.

  10. 12'' : Nõela all / Ahto Vahter

    Index Scriptorium Estoniae

    Vahter, Ahto

    1998-01-01

    Uutest plaatidest Krust "True Stories", Urban Species "Woman", The Interloper "Get Together", Mos Def & Talib Kweli "Black Star", The Brian Setzer Orchestra "The Dirty Boogie", OST "The Avengers", Jhelisa "Friendly Pressure", Ordinary People feat.Jude Mansi "The Missing", More So feat. Filthy Rich & Damon Trueitt "Take My Hand", Amira "My Desire"

  11. EURECCA colorectal: multidisciplinary mission statement on better care for patients with colon and rectal cancer in Europe

    NARCIS (Netherlands)

    Velde, C.J. van de; Aristei, C.; Boelens, P.G.; Beets-Tan, R.G.; Blomqvist, L.; Borras, J.M.; Broek, C.B. van den; Brown, G.; Coebergh, J.W.W.; Cutsem, E.V.; Espin, E.; Gore-Booth, J.; Glimelius, B.; Haustermans, K.; Henning, G.; Iversen, L.H.; Krieken, J.H. van; Marijnen, C.A.; Mroczkowski, P.; Nagtegaal, I.; Naredi, P.; Ortiz, H.; Pahlman, L.; Quirke, P.; Rodel, C.; Roth, A.; Rutten, H.J.; Schmoll, H.J.; Smith, J.; Tanis, P.J.; Taylor, C.; Wibe, A.; Gambacorta, M.A.; Meldolesi, E.; Wiggers, T.; Cervantes, A.; Valentini, V.

    2013-01-01

    BACKGROUND: Care for patients with colon and rectal cancer has improved in the last twenty years however still considerable variation exists in cancer management and outcome between European countries. Therefore, EURECCA, which is the acronym of European Registration of cancer care, is aiming at def

  12. Data of evolutionary structure change: 1DEFA-3CMDB [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1DEFA-3CMDB 1DEF 3CMD A B SVLQ---VLHIPDERLRKVAKPVE-EVN----AEIQRIV...pdbChain> 3CMDB KNSQDPIKAEE HHH HHHH ILE CA 668 3CMD B 3CMDB...MD B 3CMDB DACLGEGEGCLSV 3CMD B 3CMDB VHVPS--SLST

  13. Iron Deficiency Impairs Intra-Hepatic Lymphocyte Mediated Immune Response.

    Directory of Open Access Journals (Sweden)

    Eliano Bonaccorsi-Riani

    Full Text Available Hepatic expression of iron homeostasis genes and serum iron parameters predict the success of immunosuppression withdrawal following clinical liver transplantation, a phenomenon known as spontaneous operational tolerance. In experimental animal models, spontaneous liver allograft tolerance is established through a process that requires intra-hepatic lymphocyte activation and deletion. Our aim was to determine if changes in systemic iron status regulate intra-hepatic lymphocyte responses. We used a murine model of lymphocyte-mediated acute liver inflammation induced by Concanavalin A (ConA injection employing mice fed with an iron-deficient (IrDef or an iron-balanced diet (IrRepl. While the mild iron deficiency induced by the IrDef diet did not significantly modify the steady state immune cell repertoire and systemic cytokine levels, it significantly dampened inflammatory liver damage after ConA challenge. These findings were associated with a marked decrease in T cell and NKT cell activation following ConA injection in IrDef mice. The decreased liver injury observed in IrDef mice was independent from changes in the gut microflora, and was replicated employing an iron specific chelator that did not modify intra-hepatic hepcidin secretion. Furthermore, low-dose iron chelation markedly impaired the activation of isolated T cells in vitro. All together, these results suggest that small changes in iron homeostasis can have a major effect in the regulation of intra-hepatic lymphocyte mediated responses.

  14. Effectiveness and reliability analysis of emergency measures for flood prevention

    NARCIS (Netherlands)

    Lendering, K.T.; Jonkman, S.N.; Kok, M.

    2014-01-01

    During flood events emergency measures are used to prevent breaches in flood defences. However, there is still limited insight in their reliability and effectiveness. The objective of this paper is to develop a method to determine the reliability and effectiveness of emergency measures for flood def

  15. Molecular analysis of myophosphorylase deficiency in Dutch patients with McArdle's disease.

    NARCIS (Netherlands)

    Martin, M.A.; Rubio, J.C.; Wevers, R.A.; Engelen, B.G.M. van; Steenbergen-Spanjers, G.C.H.; Diggelen, O.P. van; Visser, M. de; Die-Smulders, C.E.M. de; Blazquez, A.; Andreu, A.L.; Arenas, J.

    2004-01-01

    We report on 8 Dutch patients with McArdle's disease from 6 unrelated families. Molecular analysis revealed the presence of four previously described mutations: the common R49X mutation, the IVS14+1G>A mutation and the recently reported R269X and Y84X nonsense mutations; and two new molecular def

  16. Developing a Data Set and Processing Methodology for Fluid/Structure Interaction Code Validation

    Science.gov (United States)

    2007-06-01

    hollow support shaft. 1.2.4.3 Vibrometer System A PolyTec laser vibrometer was used to supplement the other instrumentation for all flexible test...Anemometer (HWA) Empty Tunnel Changed CADDMAS inputs to eliminate Polytec positions for anemometer inputs...Def." -moved Polytec velocity--channel 17 (airfoil AOA accel) -moved Polytec displacement--channel 18 (airfoil flap accel

  17. Mutual redundancies in interhuman communication systems: steps towards a calculus of processing meaning

    NARCIS (Netherlands)

    L. Leydesdorff; I.A. Ivanova

    2014-01-01

    The study of interhuman communication requires a more complex framework than Claude E. Shannon's (1948) mathematical theory of communication because "information" is defined in the latter case as meaningless uncertainty. Assuming that meaning cannot be communicated, we extend Shannon's theory by def

  18. Pop / Andri Riid

    Index Scriptorium Estoniae

    Riid, Andri, 1972-

    2005-01-01

    Heliplaatidest: Machine Men "Elegies", Iggy Pop "A Million In Prizes - The Anthology", Supergrass "Road To Rouen", Erinevad esitajad "Def Jazz", Public Enemy "Power To The People And The Beats", Bratja Grimm "Bratja Grimm", Column One "Dream Time", Ezekiel Honig and Morgan Packard "Early Morning Migration"

  19. RISK ASSESSMENT AND REMEDIATION OF SOILS CONTAMINATED BY MINING AND SMELTING OF LEAD, ZINC AND CADMIUM

    Science.gov (United States)

    Mining nd smelting of Pb, Zn and Cd ores have caused widespread soil contamination in many countries. In locations with severe soil contamination, and strongly acidic soil or mine waste, ecosystems are devastated. Research has shown that An phytotoxicity, Pb-induced phosphate def...

  20. Categorization of rheological scaling models for particle gels applied to casein gels

    NARCIS (Netherlands)

    Mellema, M.; Opheusden, van J.H.J.; Vliet, van T.

    2002-01-01

    Rennet-induced casein gels made from skim milk were studied rheologically. A scaling model or framework for describing the rheological behavior of gels is discussed and used for classification of the structure of casein gels. There are two main parameters in the model that describe the number of def

  1. Autism symptoms in Attention-Deficit/Hyperactivity Disorder : A Familial trait which Correlates with Conduct, Oppositional Defiant, Language and Motor Disorders

    NARCIS (Netherlands)

    Mulligan, Aisling; Anney, Richard J. L.; O'Regan, Myra; Chen, Wai; Butler, Louise; Fitzgerald, Michael; Buitelaar, Jan; Steinhausen, Hans-Christoph; Rothenberger, Aribert; Minderaa, Ruud; Nijmeijer, Judith; Hoekstra, Pieter J.; Oades, Robert D.; Roeyers, Herbert; Buschgens, Cathelijne; Christiansen, Hanna; Franke, Barbara; Gabriels, Isabel; Hartman, Catharina; Kuntsi, Jonna; Marco, Rafaela; Meidad, Sheera; Mueller, Ueli; Psychogiou, Lamprini; Rommelse, Nanda; Thompson, Margaret; Uebel, Henrik; Banaschewski, Tobias; Ebstein, Richard; Eisenberg, Jacques; Manor, Iris; Miranda, Ana; Mulas, Fernando; Sergeant, Joseph; Sonuga-Barke, Edmund; Asherson, Phil; Faraone, Stephen V.; Gill, Michael

    2009-01-01

    It is hypothesised that autism symptoms are present in Attention-Deficit/Hyperactivity Disorder (ADHD), are familial and index subtypes of ADHD. Autism symptoms were compared in 821 ADHD probands, 1050 siblings and 149 controls. Shared familiality of autism symptoms and ADHD was calculated using DeF

  2. A new method to measure bowen ratios using high resolution vertical dry and wet bulb temperature profiles

    NARCIS (Netherlands)

    Euser, T.; Luxemburg, W.; Everson, C.; Mengistu, M.; Clulow, A.; Bastiaanssen, W.

    2013-01-01

    The Bowen ratio surface energy balance method is a relatively simple method to determine the latent heat flux and the actual land surface evaporation. Despite its simplicity, the Bowen ratio method is generally considered to be unreliable due to the use of two-level sensors that are installed by def

  3. Fabrication of Co/Si nanowires by ultrahigh-vacuum scanning tunneling microscopy on hydrogen-passivated Si(100) surfaces

    NARCIS (Netherlands)

    Palasantzas, G; Ilge, B; De Nijs, J

    1999-01-01

    We have fabricated nanometer width Co/Si metal lines on Si(100) surfaces by ultrahigh-vacuum scanning tunneling microscopy (UHVSTM) based nanolithography on the hydrogen-passivated surface, combined with vapor deposition of Co at room temperature and subsequent annealing. The STM tip was used to def

  4. Pop / Andri Riid

    Index Scriptorium Estoniae

    Riid, Andri, 1972-

    2005-01-01

    Heliplaatidest: Machine Men "Elegies", Iggy Pop "A Million In Prizes - The Anthology", Supergrass "Road To Rouen", Erinevad esitajad "Def Jazz", Public Enemy "Power To The People And The Beats", Bratja Grimm "Bratja Grimm", Column One "Dream Time", Ezekiel Honig and Morgan Packard "Early Morning Migration"

  5. The Future Is Unwritten. Joe Strummer. White label : Fuck yuo I am a robot. Kuula / DJ Pickney Tiger

    Index Scriptorium Estoniae

    DJ Pickney Tiger, pseud., 1970-

    2007-01-01

    Heliplaatidest: "The Future Is Unwritten. Joe Strummer", The Nightwatchman "One Man Revolution", Def Räädu & J.O.C. "Succiety2", The Joshi Butler Trio "Grand National", "Biitparaadid 2", The Police "The Police", Laurie Anderson "Big Science", Dreamtheater "Systematic chaos", Ghosts "The World is Outside", Tokio Hotel "Scream", Bon Jovi "Lost Highway", Megadeth "United Abominations", Marilyn Manson "Eat Me, Drink Me"

  6. Planning and Conducting Offensive Counterinformation Operations

    Science.gov (United States)

    2002-04-01

    doctrine calls this special information operations (SIO)) which precludes timely data sharing and collaboration amongst the proper experts, planners, and...is classified as special information operations. Offensive information operations are conducted across the range of military operations while...Operations RF Radio Frequency ROE Rules of Engagement SecDef Secretary of Defense SIO Special Information Operations SOF Special Operations Force

  7. Petunia floral defensins with unique prodomains as novel candidates for development of fusarium wilt resistance in transgenic banana plants.

    Directory of Open Access Journals (Sweden)

    Siddhesh B Ghag

    Full Text Available Antimicrobial peptides are a potent group of defense active molecules that have been utilized in developing resistance against a multitude of plant pathogens. Floral defensins constitute a group of cysteine-rich peptides showing potent growth inhibition of pathogenic filamentous fungi especially Fusarium oxysporum in vitro. Full length genes coding for two Petunia floral defensins, PhDef1 and PhDef2 having unique C-terminal 31 and 27 amino acid long predicted prodomains, were overexpressed in transgenic banana plants using embryogenic cells as explants for Agrobacterium-mediated genetic transformation. High level constitutive expression of these defensins in elite banana cv. Rasthali led to significant resistance against infection of Fusarium oxysporum f. sp. cubense as shown by in vitro and ex vivo bioassay studies. Transgenic banana lines expressing either of the two defensins were clearly less chlorotic and had significantly less infestation and discoloration in the vital corm region of the plant as compared to untransformed controls. Transgenic banana plants expressing high level of full-length PhDef1 and PhDef2 were phenotypically normal and no stunting was observed. In conclusion, our results suggest that high-level constitutive expression of floral defensins having distinctive prodomains is an efficient strategy for development of fungal resistance in economically important fruit crops like banana.

  8. Classification of H^2-FUNCTIONS According to the Degree of Their Cyclicity

    Science.gov (United States)

    Vasyunin, V. I.; Nikol'skiĭ, N. K.

    1984-04-01

    The vector-valued functions f in the Hardy space H^2(E) are classified according to their approximation capabilities with respect to the backward shift operator S^*, S^*f\\overset{\\operatorname{def}}{=}(f-f(0))/z, i.e., according to the "size" of the closed linear span \\operatorname{span}(S^{*k}f\\colon k\\ge0).Bibliography: 6 titles.

  9. Plant responses to insect egg deposition

    NARCIS (Netherlands)

    Hilker, M.; Fatouros, N.E.

    2015-01-01

    Plants can respond to insect egg deposition and thus resist attack by herbivorous insects from the beginning of the attack, egg deposition. We review ecological effects of plant responses to insect eggs and differentiate between egg-induced plant defenses that directly harm the eggs and indirect def

  10. Molecular cloning and functional characterization of duck nucleotide-binding oligomerization domain 1 (NOD1).

    Science.gov (United States)

    Li, Huilin; Jin, Hui; Li, Yaqian; Liu, Dejian; Foda, Mohamed Frahat; Jiang, Yunbo; Luo, Rui

    2017-09-01

    Nucleotide-binding oligomerization domain 1 (NOD1) is an imperative cytoplasmic pattern recognition receptor (PRR) and considered as a key member of the NOD-like receptor (NLR) family which plays a critical role in innate immunity through sensing microbial components derived from bacterial peptidoglycan. In the current study, the full-length of duck NOD1 (duNOD1) cDNA from duck embryo fibroblasts (DEFs) was cloned. Multiple sequence alignment and phylogenetic analysis demonstrated that duNOD1 exhibited a strong evolutionary relationship with chicken and rock pigeon NOD1. Tissue-specific expression analysis showed that duNOD1 was widely distributed in various organs, with the highest expression observed in the liver. Furthermore, duNOD1 overexpression induced NF-κB activation in DEFs and the CARD domain is crucial for duNOD1-mediated NF-κB activation. In addition, silencing the duNOD1 decreased the activity of NF-κB in DEFs stimulated by iE-DAP. Overexpression of duNOD1 significantly increased the expression of TNF-α, IL-6, and RANTES in DEFs. These findings highlight the crucial role of duNOD1 as an intracellular sensor in duck innate immune system. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. A Big Data-inspired Spatio-temporal Story Model for Forensic Evidence Validation

    OpenAIRE

    2013-01-01

    Digital Forensic Evidence (DFE) are more than digitised computer data chunks. They may contain complicated causality relationships within specific timelines and feature domains. Recent developments in forensic science study have shown that DEF should be treated as part of the concept of “Big Data” formed by the explosively increasing electronic data generated by Internet, social networks, consumer electronics, and public surveillance systems.

  12. POOR REPRODUCTION IN FOREST PASSERINES FROM DECLINE OF SNAIL ABUNDANCE ON ACIDIFIED SOILS

    NARCIS (Netherlands)

    GRAVELAND, J; VANDERWAL, R; VANBALEN, JH; VANNOORDWIJK, AJ

    1994-01-01

    ON poor, acidified soils in The Netherlands, an increasing number of great tits, Parus major, and other forest passerines, produce eggs with thin and porous shells1. Here we show that the egg-shell defects, and the related high incidence of clutch desertion and empty nests, are caused by calcium def

  13. Pop / Tristan Priimägi

    Index Scriptorium Estoniae

    Priimägi, Tristan, 1976-

    2008-01-01

    Heliplaatidest: Elbow "The Seldom Seen Kid", Lahesõda "Dermocracy", Nits "Doing The Dishes", Nine Inch Nails "Ghosts I-IV", The Last Shadow Puppets "The Age Of The Understatement", The Shortwave Set "Replica Sun Machine", Def Leppard "Songs From The Sparkle Lounge"

  14. The Future Is Unwritten. Joe Strummer. White label : Fuck yuo I am a robot. Kuula / DJ Pickney Tiger

    Index Scriptorium Estoniae

    DJ Pickney Tiger, pseud., 1970-

    2007-01-01

    Heliplaatidest: "The Future Is Unwritten. Joe Strummer", The Nightwatchman "One Man Revolution", Def Räädu & J.O.C. "Succiety2", The Joshi Butler Trio "Grand National", "Biitparaadid 2", The Police "The Police", Laurie Anderson "Big Science", Dreamtheater "Systematic chaos", Ghosts "The World is Outside", Tokio Hotel "Scream", Bon Jovi "Lost Highway", Megadeth "United Abominations", Marilyn Manson "Eat Me, Drink Me"

  15. Technical Risk Assessment. The Status of Current DoD Efforts

    Science.gov (United States)

    1986-04-01

    Offices giving inconsistent definitions4 Office,, c., nqr no def inition Wo entered definituins in thle tale if all or1 104 st Iresp loltts pro vidIed...altitudes. Thbe Army epsMdinterest in an adIvance’d scout hielicoliter tin 197.1 bait decided 5 years later t hat an entirely new helicopter wsnot attcrd

  16. Regeneration of gill lamellae of the Indian horseshoe crab, Tachypleus gigas (Muller)

    Digital Repository Service at National Institute of Oceanography (India)

    Chatterji, A.; Alam, H.; Joshi, B.K.; Bhonde, R.R.

    ,5,6 , but def i nite Ginkgoales were recorded from Jurassic beds of R a jmahal Basin 7 . Many forms of leaves and ov ules of Ginkgo from the Early Cretaceous d e- posits are virtually unchanged till mo d ern time. The order Ginkgoales in the Mesozoic...

  17. Expression profile analysis of early fruit development in iaaM-parthenocarpic tomato plants

    Directory of Open Access Journals (Sweden)

    Spena Angelo

    2009-07-01

    Full Text Available Abstract Background Fruit normally develops from the ovary after pollination and fertilization. However, the ovary can also generate seedless fruit without fertilization by parthenocarpy. Parthenocarpic fruit development has been obtained in tomato (Solanum lycopersicum by genetic modification using auxin-synthesising gene(s (DefH9-iaaM; DefH9-RI-iaaM expressed specifically in the placenta and ovules. Findings We have performed a cDNA Amplified Fragment Length Polymorphism (cDNA-AFLP analysis on pre-anthesis tomato flower buds (0.5 cm long collected from DefH9-iaaM and DefH9-RI-iaaM parthenocarpic and wild-type plants, with the aim to identify genes involved in very early phases of tomato fruit development. We detected 212 transcripts differentially expressed in auxin-ipersynthesising pre-anthesis flower buds, 65 of them (31% have unknown function. Several differentially expressed genes show homology to genes involved in protein trafficking and protein degradation via proteasome. These processes are crucial for auxin cellular transport and signaling, respectively. Conclusion The data presented might contribute to elucidate the molecular basis of the fruiting process and to develop new methods to confer parthenocarpy to species of agronomic interest. In a recently published work, we have demonstrated that one of the genes identified in this screening, corresponding to #109 cDNA clone, regulates auxin-dependent fruit initiation and its suppression causes parthenocarpic fruit development in tomato.

  18. Effect Linn ts of aq n on re queous eprodu s leave uctive es extr ...

    African Journals Online (AJOL)

    SAM

    Effect. Linn. Departmen. Wide usage male albino the sperm. Thirty six (3 of the treate .... adversely affect male fertility (Olayemi, 2010). Many antimalarial drugs have been implicated in male infertility. For instance .... d humans. Th ... bee. (Oy. M def syn phy of d has tiology of th esence of a ese alkaloids .... plant foods. Hum.

  19. Graafikatriennaali peapreemia Andres Talile

    Index Scriptorium Estoniae

    2001-01-01

    Tallinna XII graafikatriennaali preemiad: grand prix - Andres Tali; 3 võrdset preemiat - Janne Laine, Leonards Laganovskis, Peeter Laurits; diplomid - Lynne Allen, Lucrecia Urbano, Vladimir Tshaika, Zora Stanè, Endi Poskovic, Päivikki Kallio; EKM preemia - Tif Bitmap; Tallinna linna preemia - Ülle Marks ja Jüri Kass; eripreemiad: - J. Laine (Eesti Pank), Vive Tolli (Paletti Eesti AS), Martins Ratniksh, Juris Boiko, T. Bitmap (Balteco AS). Žürii koosseis

  20. National Program for Inspection of Non-Federal Dams. Dufresne Dam (VT 00144) Upper Hudson River Basin, Manchester, Vermont. Phase I Inspection Report.

    Science.gov (United States)

    1979-01-01

    operator. This dam is maintained and operated by the State of Vermont Fish and Game Department. The State’s Dam Safety Engineer is Mr. A. Peter Barranco , Jr...P.E. Mr. Barranco is located at the Agency of Environmental I Conservation, Department of Water Resources, Water Quality Division, Montpelier...Stan Mfazur, HNTB 7. I 3. Dan LaGatta, GEI 8. 4. J. Peter Barranco , Jr. -Vermont Dept. 9. of Water Resources- 5

  1. Minutes of Workshop on Composite Materials Test Methods Held at the Institute for Defense Analyses on February 27, 1985.

    Science.gov (United States)

    1985-04-01

    Appendix 4). NDE was used to character- ize the panels; void content of 2% maximum was specified for the test panels. The publication of this...HEXCEL (415) 823-4200 CHANNON, STAN IDA (703) 845-2256 (714) 683-7357 CLEVINGER, Gary Babcock & Wilcox (804) 522-5286 DAVIS, JOHN G., JR NASA-LANGLEY...suppliers, contractors and ASNT. Yes Yes Yes Only from a Standardization/Documentation standpoint. No Yes Not to define "the" method since NDE is

  2. Implementing reliable Web services

    OpenAIRE

    Koskipää, Otto

    2012-01-01

    Web services are a common and standard way to implement communication between information systems and provide documented interfaces. The Web services are usually using SOAP because it is a widely-spread, well-documented and used standard. SOAP standard defines a message structure, an envelope, that is sent over internet using HTTP and contains XML data. An important part of the SOAP structure is the exception mechanism that returns a Fault element in the response. The SOAP Fault is a stan...

  3. Signing Ceremony Between CAS and Schneider Electric Held in Beijing

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ On April 9, 2008, a strategic cooperation signing ceremony between the China Association for Stan-dardization (CAS) and Schneider Electric took place at the Diao Yutai Guest Hotel in Beijing. Attending the signing ceremony were Mr Sun Xiaokang, Duty Director of SAC, Mr. Ma Lingcong, Secretary General of CAS, Mr. Noel Girard, Schneider Electric Marketing Director, Mr. Guy Dufrdi sse, Chairman of Schneider Electric (China) and more than twenty high-ranking managers from both companies.

  4. Uus plaat

    Index Scriptorium Estoniae

    2002-01-01

    Uutest plaatidest Bobby McFerrin "Beyond Words", Terri Lyne Carrington "Jazzs A Sirit", Bugge Wesseltorf "Moving", Richard Bona " Reverence", "Rull's Royce Rull's Choice", Trilok Gurtu "The Beat Of Love", Stan Getz "Getz For Lovers", Billy Cobham, "Drum'n'Voice - All That Groove", Pat Metheny "Speacing Of Now", Kimmo Pohjonen "Kluster", Fred Frith "Digital Wildlife", Uri Caine "Rio", Bobby Previte. Bump The Renaissance Band "Just Add Water", Keith Jarrett, Gary Peacock, Jack DeJohnette "Inside Out"

  5. USSR Report: Cybernetics, Computers and Automation Technology. No. 69.

    Science.gov (United States)

    1983-05-06

    Investigations must subsequently be organized to develop universal multilanguage programming systems that guarantee automa- tion of all phases of...the 26th CPSU Congress," organized by the Sevastopol Branch of RDENTP [not further identified] of the Ukrainian SSR Znaniye Society , the...Ail-Union Scientific Research Institute of Stan- dardization and by the Kiev city Soviet of the NTO [Scientific and technical society ], was held in

  6. Uus plaat

    Index Scriptorium Estoniae

    2002-01-01

    Uutest plaatidest Bobby McFerrin "Beyond Words", Terri Lyne Carrington "Jazzs A Sirit", Bugge Wesseltorf "Moving", Richard Bona " Reverence", "Rull's Royce Rull's Choice", Trilok Gurtu "The Beat Of Love", Stan Getz "Getz For Lovers", Billy Cobham, "Drum'n'Voice - All That Groove", Pat Metheny "Speacing Of Now", Kimmo Pohjonen "Kluster", Fred Frith "Digital Wildlife", Uri Caine "Rio", Bobby Previte. Bump The Renaissance Band "Just Add Water", Keith Jarrett, Gary Peacock, Jack DeJohnette "Inside Out"

  7. Proceedings of a Workshop on Distributed Sensor Nets Held at Pittsburgh, Pennsylvania on December 7-8, 1978

    Science.gov (United States)

    1978-12-01

    to know enough about the activity of * by a history Of the Previous states, by the overall comunication Itself to determine when the focus of...must be considered in the design and operation of a real distributed Figure 3.8. Clasification Contract Coemmunlcatlon. sensing system. Most of these...34 Transportation Research Record, Report Based System for the :nterpretation of Protein X- 596. ray Crystallographic Data," Technical Report Stan- ඨ. CS-77-589

  8. Evaluation of Different Features for Face Recognition in Video

    Science.gov (United States)

    2014-09-01

    Agency Ottawa ON Canada K1A 0L8 Erico Neves, Stan Matwin University of Ottawa, School of Electrical Engineering and Computer Science 161 Louis ... Pasteur Ottawa ON Canada K1N 6N5 Eric Granger Université Québec 1100, rue Notre-Dame Ouest Montréal (Québec) H3C 1K3 Scientific Authority: Pierre

  9. From Reputation Models and Systems to Reputation Ontologies

    OpenAIRE

    Alnemr, Rehab; Meinel, Christoph

    2011-01-01

    Part 2: Full Papers; International audience; Reputation has been explored in diverse disciplines such as artificial intelligence, electronic commerce, peer-to-peer network, and multi-agent systems. Recently it has been a vital component for ensuring trust in web services and service oriented architectures domains. Although there are several studies on reputation systems as well as reputation models, there is no study that covers reputation ontologies especially the ones implemented using stan...

  10. Nature-based tourism and nature protection: quality standards for travelling in protected areas in the Alps. eco.mont (Journal on Protected Mountain Areas Research)|eco.mont Vol. 9 special issue|

    OpenAIRE

    Siegrist, Dominik; Ketterer Bonnelame, Lea

    2017-01-01

    Nature-based tourism is an important element of ecological and sustainable development in the Alps. A functioning quality management programme is essential to ensure high-quality nature-based tourism. The authors developed ten quality standards, differentiated by five quality-related criteria with associated indicators. Standards were developed by involving experts from all Alpine countries and using an Alpine-wide online survey; the standards were tested in six case studies. The quality stan...

  11. Study and Improvement of WTLS Protocol in WAP

    Institute of Scientific and Technical Information of China (English)

    CHEN Kai; LIU Ying; XIAO Guozhen

    2001-01-01

    Wireless application protocol (WAP)is a set of protocol designed for mobile terminal toconnect to Internet and is likely to become world stan-dard. Wireless transport layer security (WTLS) incor-porates the security features in WAP. In this paper,the analysis of the performance of WTLS is made.Two improvement schemes on WTLS are given: oneis that the client stores the certificate of servers; theother is that a new WTLS protocol is proposed basedon the designed authentication scheme.

  12. Stemcell Information: SKIP001177 [SKIP Stemcell Database[Archive

    Lifescience Database Archive (English)

    Full Text Available u Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA. Stan...ford Cardiovascular Institute, Stanford University School of Medicine, Stanford, ...California, USA. Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, Califo...rnia, USA. Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, ...titute, Stanford University School of Medicine, Stanford, California, USA. Stanford Cardiovascular Institute, Stanford University Sch

  13. India’s Changing Afghanistan Policy: Regional and Global Implications

    Science.gov (United States)

    2012-12-01

    Afghanistan has also sought Indian aid in agri-technology, which would halt desertifica- tion, deforestation, and water wastage in Afghani- stan.15...Afghanistan was self-sufficient in food until the 1970s, but since then the vagaries of war, drought, the growth of the drug trade, and mismanagement have... Food Programme. India is also funding and executing the Salma Dam Power Project in Heart Province involving a commitment of around $80 mil- lion as

  14. Laser-Deposited In Situ TiC-Reinforced Nickel Matrix Composites: 3D Microstructure and Tribological Properties (Postprint)

    Science.gov (United States)

    2014-04-03

    was measured using a stan- dard Vickers microhardness tester using a 300-g load. Sliding friction and wear testing was con- ducted with a Falex (Implant...exported to AvizoFire 6.3 format for 3D visualization of TiC and graphite reinforcements. Microhardness and Tribological Properties The microhardness ...Sciences) ISC-200 pin- on-disk (POD) system at room temperature. The samples were openly exposed in lab air (40% RH) during the tests . Tests were

  15. Using New Technologies: A Technology Transfer Guidebook. Version 02.00. 08

    Science.gov (United States)

    1993-12-01

    In a June 1992 IEEE Spectrum article, Capers Jones (1992) recounted aCASEWorld conference presentation by Chuck House, a former executive of Hewlett...Addison-Wesley. Implementation Management Accelerating Change Workshop. Brighton, Colorado: IMA, Inc. Associates 1992 Jones, Capers CASE’s Missing...Technology, Stan Przybylinski and Priscilla J. Fowler, eds., Santa Barbara , California (November 15-16, 1987):63-66. CASE Consulting Group. CASE Outlook

  16. The ’Triangle of Death’: Medical Sustainability in Expeditionary Sea-Based Operations

    Science.gov (United States)

    2008-01-01

    reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information...surprise—will presumably target the afloat sustainment base, whose big , slow ships include prepositioned units with hulls not built to combat stan- dards...See Arthur M. Smith, “Has the Red Cross–Adorned Hospital Ship Become Obso - lete?” Naval War College Review 58, no. 3 (Summer 2005), pp. 121–31. 25

  17. GB7718-2004预包装食品标签通则(节选)

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ 前言 本标准的5.3是推荐性的,其余为强制性的.本标准非等效采用国际食品法典委员会(CAC)CODEX STAN 1-1985(1991、1999修订),同时参考了美国联邦法规§101部分.

  18. GB7718-2004预包装食品标签通则(节选)

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    前言  本标准的5.3是推荐性的,其余为强制性的.本标准非等效采用国际食品法典委员会(CAC)CODEX STAN 1-1985(1991、1999修订),同时参考了美国联邦法规§101部分.……

  19. Proceedings of the Joint Logistics Commanders Joint Policy Coordinating Group on Computer Resource Management; Computer Software Management Subgroup. Second Software Workshop, 22-25 June 1981

    Science.gov (United States)

    1981-11-01

    RUSO ) Within each system or software producing entity, both industry and government, an organizational structure must be established which specif...icaely addresses the reuse of software. The objectives of the RUSO Pre to establish an effective company-wide reuse program. The goals are: reduced...usually offer the highest payoff for stan- dardization efforts. Upon identifying areas for standardization, the RUSO should evaluate sup- port

  20. The Coast Guard Proceedings of the Marine Safety and Security Council. Volume 72, Number 2, Summer 2015

    Science.gov (United States)

    2015-01-01

    GNSS , Inertial, and Multi-sensor Integrated Navigation Systems On-Line Appendix C, Historical Navigation Systems. Virtual buoys may present...mission for Maritime Services ( RTCM ) and a very inclusive group of stakeholders, including: ► the America Pilots’ Association, ► the International...Traditionally, RTCM standards efforts for ship- board navigation and radio communication equip- ment and systems have focused on applying stan- dards for

  1. Notice

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    No responsibility is assumed by China Journal of Accounting Research nor Elsevier for any injury and/or damage to persons,property as a matter of product liability,negligence,or otherwise,or from any use or operation of any methods,products,instructions,or ideas contained in the material herein.Although all advertising material is expected to conform to ethical stan-

  2. Population Health in Regions Adjacent to the Semipalatinsk Nuclear Test Site

    Science.gov (United States)

    1998-09-01

    as well rent standard units, AFRRI did not collaborate in as the entire infrastructure of the largely agrarian this report. Editorial changes to...ma- were signed that shaped potential collaboration by terials are unique. Current data on population health the two countries in environmental studies...exceed 1950s and 1960s and by an interinstitutional com- the values stated in current Radiation Safety Stan- mission headed by academician A.F. Tzyb

  3. IEC and China Industrial Automation

    Institute of Scientific and Technical Information of China (English)

    Mei Ke

    2006-01-01

    @@ A long with the development of global economy and science technology, IEC international standards as the important technical trade measure are taking on more and more prominence in world trade. Under consideration of international competition, many countries joint in IEC works in process actively and make effort on consistent between national stan dards and IEC standards, in order to obtain the advantage position within the international competition.

  4. Influence of Microstructure on Micro-/Nano-Mechanical Measurements of Select Model Transparent Poly(urethane urea) Elastomers

    Science.gov (United States)

    2012-12-17

    industrial, medical, and military applications, particularly in the areas of coating , adhesives, foams, and composite structures [1]. More specifically, high...HMX probes were calibrated prior to each scan using a stan- dard sample composed of a copolymer of polystyrene and low- density polyethylene (PSeLDPE...scattering ( WAXS ) (not shown), where results from the latter indicated only a broad halo with a Bragg spacing of w4.4 A, confirming the amorphous

  5. Employing Industry Standards for System Integration in Digital Radiology Department

    Institute of Scientific and Technical Information of China (English)

    Yongjian Bao

    2004-01-01

    This paper reviews system inte-gration needs in a digital radiologydepartment, and open industry stan-dards developed in the past twodecades to address these needs. In par-ticular, the Integrating the HospitalEnterprise (IHE) initiative defines aset of Integration Profiles in radiolo-gy, with a focus on the interoperabili-ty of the integrated systems, based onexisting industry standards, such asDICOM and HL7.

  6. Intraclonal variation in defence substances and palatability: a study on Carex and lemmings

    OpenAIRE

    Bråthen, KA; Agrell, Jep; Berteaux, D.; Jonsdottir, IS

    2004-01-01

    Clonal sedges consist of integrated ramets at different development stages. Many of these sedges are important food for herbivores, yet differences in herbivore preferences and defence allocation between ramet development stages have not previously been evaluated. In this study we investigated intraclonal ramet variation in level of plant defence and nutrient compounds and intraclonal ramet preferences by lemmings (Lemmus trimucronatus) in field samples of a rhizomatous sedge (Carex stans). P...

  7. Analysis of the Burning Region and Plume of a Large Fire

    Science.gov (United States)

    1986-09-30

    Carrier, Fendell and Feldman, 1982], rotational forces are neglected in this analysis. The experimental work of McCaffrey [19791 and the analyses of Murgal...F. E. Fendell , and P. S. Feldman, Firestorms, TRW Space and Technology Group, Redondo Beach, California, Document 38163-6001-UT-00, 1982. Cermak, J...ATTN: C WILTON SRI INTERNATIONAL ATTN: G ABRAHAM~SON STAN MARTIN ASSOCIATES ATTN: S MARTIN SWETL, INC ATTN: T PALMER TRW ELECTRONICc- & DEFENSE SECTOR ATTN: F FENDELL -1

  8. The PARTI Architecture Assurance

    Science.gov (United States)

    2008-10-01

    example safety critical system, 2, Issues Guidance Papers ( IGPs ) that further explain key concepts or requirements of the STAN- DARD, The guidance...Organisation (2009) IGP -OOl: Guidance Notes for Project Offices. Published as part of [20]. 4. Defence Material Organisation (2009) IGP -002: Methodsfor Safety...Architecture Analysis. Published as part of [20]. 5. Defence Material Organisation (2009) IGP -003: Methods for Design Assurance. Published as part of

  9. The Framing of Network Neutrality Governance

    DEFF Research Database (Denmark)

    Perry, James

    The neutrality of the internet with regard to applications (e.g. search, social networking, email, to mention only three) has been central to innovation and growth in the knowledge-economy over the past two decades. Until recently, neutrality was built into the internet's design via its core stan...... with a critical analysis of their respective operational paradigms, the paper seeks to understand who is framing the debate, how they are doing so, and to what (systemic) effect....

  10. A Critical Review on Singapore English

    Institute of Scientific and Technical Information of China (English)

    谌婷

    2013-01-01

    A critical review is given on Singapore English. The historical introduction of the language provides readers a longitu-dinal insight into the development of this special variety. Then, the language features of Colloquial Singapore English and Stan-dard Singapore English are discussed, and a comparison of the use of Singapore and standard British English is made to show the differences in grammatical and lexical features of these two varieties of English.

  11. Defense AT and L. Volume 42, Number 1

    Science.gov (United States)

    2013-02-01

    56 Building Genuine Motivation Stan Emelander 59 How Full Is the Toolbox? Michael A. Bayer 14 LOG 211 Supportability Analysis Course Now...groups and/ or systems IATAC POC: Rogelio Raymond (703)984-0072 or raymond_rogelio@bah.com The u.s. Department of Defense science and Technology...February 2013 : – How Full Is the Toolbox? A Look at Product Support Analysis Tools Michael A. Bayer Bayer is a professor of Life Cycle Logistics

  12. Semi-structured interview is a reliable and feasible tool for selection of doctors for general practice specialist training

    DEFF Research Database (Denmark)

    Isaksen, Jesper; Hertel, Niels Thomas; Kjær, Niels Kristian

    2013-01-01

    In order to optimise the selection process for admission to specialist training in family medicine, we developed a new design for structured applications and selection interviews. The design contains semi-structured interviews, which combine individualised elements from the applications with stan...... with standardised behaviour-based questions. This paper describes the design of the tool, and offers reflections concerning its acceptability, reliability and feasibility....

  13. Self-selection of standards by children: The relative effectiveness of pupil-selected and teacher-selected standards of performance

    OpenAIRE

    Dickerson, E. Anne; Creedon, Carol F.

    1981-01-01

    This study examines the effects of contingent reinforcement under conditions of pupil-selected and teacher-selected performance standards upon pupils' academic response rates. The academic response rate was measured by the number of correct responses emitted per session. Thirty pupils (15 second-graders and 15 third-graders) were randomly assigned to one of three experimental groups, based on matched triplets. One group worked under pupil-selected standards; the second group worked under stan...

  14. Research on International Compatibility of China's Information Policies and Regulations%我国信息政策法规国际兼容性研究--电子政府

    Institute of Scientific and Technical Information of China (English)

    邱晓琳

    2003-01-01

    The article discusses the development of China's e-government and its compatibility with internationalstandard and practice from 5 aspects: the significance of the e-government; the definition of the e-government; analysis ofthe compatibility of China's e-government with international standard and practice; analysis of the cause; and measures toimprove the compatibility of China's e-government with international stan dard and practice.

  15. Who Is the Real Engineer?

    Science.gov (United States)

    2011-04-01

    Army engineers solve problems. They are not asked to simply analyze and report but to analyze, plan, organize, build, and deliver solutions using the...stan- dard that results in electrocution , fire, structural collapse, failed force protection measure, or any other engineering project failure...supplying only the commander’s intent); and devel- oping scenarios without stark black- and-white solutions . Such training not only develops problem solvers

  16. adidas Originals Snakeskin Pack蛇皮潮流降临

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Adidas Originals于前段时间推出了一款复古做旧的蛇皮鞋面Campus,日前Adidas Originals方面公布了全系列Snakeskin Pack,除了当中的灰色Campus Vintage外,还包括了红色蛇皮面的Superstar 80's以及黑色鞋面的Stan Smith。

  17. Environmental stewardship for gold mining in tropical regions

    OpenAIRE

    A.Isahak; S. Surif; Gill, A; J. Phang

    2013-01-01

    Mining has gained strong popularity in recent years due to the increase in global demand for metals and other industrial raw material derived from the ground. However, information and good governance regarding activities related to mining is still very much lacking especially in underdeveloped and developing countries in the tropics. In Malaysia, the importance of environmental stewardship in mining is a new phenomenon. The new National Mineral Policy 2 calls for compliance with existing stan...

  18. Predictors of adherence to different types and doses of supervised exercise during breast cancer chemotherapy.

    Science.gov (United States)

    Courneya, Kerry S; Segal, Roanne J; Gelmon, Karen; Mackey, John R; Friedenreich, Christine M; Yasui, Yutaka; Reid, Robert D; Proulx, Carolyn; Trinh, Linda; Dolan, Lianne B; Wooding, Evyanne; Vallerand, James R; McKenzie, Donald C

    2014-07-06

    Exercise is beneficial for breast cancer patients during chemotherapy but adherence to different types and doses of exercise is a challenge. The purpose of this study was to examine predictors of adherence to different types and doses of exercise during breast cancer chemotherapy in a multicenter randomized controlled trial. Breast cancer patients in Edmonton, Vancouver, and Ottawa, Canada receiving chemotherapy (N = 301) were randomized to a standard dose of 25-30 minutes of aerobic exercise (STAN), a higher dose of 50-60 minutes of aerobic exercise (HIGH), or a higher dose of 50-60 minutes of combined aerobic and resistance exercise (COMB). Predictors included demographic, medical, fitness, and quality of life variables. Exercise adherence was measured as the percentage of supervised exercise sessions completed. Overall adherence to the supervised exercise sessions was 73% (SD = 24%). In a multivariate regression model, six independent predictors explained 26.4% (p adherence. Higher exercise adherence was achieved by breast cancer patients in Vancouver (p adherence. For disease stage, patients with stage I/IIa disease adhered equally well to all three exercise interventions whereas patients with stage IIb/III disease adhered better to the STAN intervention than the two higher dose exercise interventions. For body mass index, healthy weight patients adhered equally well to all three exercise interventions whereas overweight patients adhered best to STAN and worst to COMB; and obese patients adhered best to STAN and worst to HIGH. Determinants of exercise adherence in breast cancer patients receiving chemotherapy are multidisciplinary and may vary by the exercise prescription.

  19. Towards LHC physics with nonlocal Standard Model

    OpenAIRE

    Tirthabir Biswas; Nobuchika Okada

    2015-01-01

    We take a few steps towards constructing a string-inspired nonlocal extension of the Standard Model. We start by illustrating how quantum loop calculations can be performed in nonlocal scalar field theory. In particular, we show the potential to address the hierarchy problem in the nonlocal framework. Next, we construct a nonlocal abelian gauge model and derive modifications of the gauge interaction vertex and field propagators. We apply the modifications to a toy version of the nonlocal Stan...

  20. Standardization of Aircraft Control and Performance Symbology on the USAF Head-Up Display

    Science.gov (United States)

    1993-11-01

    Mr. Loran Haworth viii uMN Capt. (Rot.) "Hawkeye" Hughes Mr. Curt Sandier Mr. Gary Kessler Mr. Richard Huff Lt. Jennifer McGovern Mr. Dan Eksusian Mr...Branch. The authors would like to extend special thanks to the following individuals for their support of these studies: Joe Fischer , Dan Bauer and...Carolyn Oakley (statistical analyses), Christy Fischer (manuscript preparation), Beverly Johnson (graphics programming support), and SSgt. Stan Singleton

  1. Aceptabilidad de empanizados enriquecidos con harina de pota (Dosidicus gigas), huevo de codorniz (Coturnix coturnix) y pimiento amarillo (Capsicum annuum)

    OpenAIRE

    Guerrero Hurtado, Emma Del Rosario; Palomino Pezzutti, Ricardo Ramiro; Tamariz Grados, Nelly Norma; Cajaleón Asencios, Delia Haydee; Dextre Mendoza, Rodolfo Willian; Carreño Mundo, Humberto

    2015-01-01

    Objetivo: Evaluar la aceptabilidad de empanizados enriquecidos con harina de pota (Dosidicus gigas), huevo de codorniz (Coturnix coturnix) y pimiento amarillo (Capsicum annuum). Métodos: Optimizar una formulación de alimento listo para el consumo humano, conforme a requisitos: NTP NDECOPI: Harina de trigo, NTP 205. 027. 1996, NTP-CODEX STAN 166:2014 -Barritas, Porciones y Filetes de pescado empanizados o rebozados congelados. Resultados: El aroma, color y textura de los empanizados formula...

  2. The effect of lateral internal sphincterotomy on resting anal sphincter pressures

    OpenAIRE

    Peker, Kemal; YILMAZ, İsmayil; DEMİRYILMAZ, İsmail; İNAL, Abdullah; Işik, Arda

    2014-01-01

    Anal fissures are one of the most common proctologic disorders. This study aimed to investigate alterations in anal sphincteric resting pressures after lateral internal sphincterotomy, which was performed for chronic anal fissure treatment. Materials and methods: Fifty-six (68.3%) male and 26 (31.7%) female patients were included in this study. Patients complicated with fistula-in-ano, abscess, cancer, inflammatory bowel disease, dermatitis, poor general condition, allergy to any of the stan...

  3. SSC San Diego Command History Calendar Year 2007

    Science.gov (United States)

    2008-03-01

    Scrimpsher, Luis Biaggi, Mitchell Gillette, Chew Hom, Michael Gonzales, Randall Smith, Vivian Chu, James Hlava, Eric Rupert, Ellen Ward, Manuel Silveira...Hamaguchi, Al Cassedy, Paul Lichtenstein, David Stevens, Stan Durbin, Ed Van Houten, Grely Ponce , Dr. Randy Moore, Larry Mohr, Mike Phillips, Andy...Michelle Sund, Larry Campbell, Bob Franco, Rick Ponce , Ron Diaz, Douglas Hovermale, Monica Dukes, George Vajgrt, Mike Hammock, Jim Logsdon, Henry

  4. IFRS Handbook for SMEs in Finland

    OpenAIRE

    Farley, Andre

    2016-01-01

    Globalisation has led to greater competition among businesses the world over to attract stakeholders who can positively affect their business on international and global markets. To facilitate competition financial statements should be understandable and comparable to similar businesses. IFRS has become the de facto financial reporting stan-dard of the world. Finnish SMEs involved in international trade are not using IFRS, though a simple version exist for SMEs the switch from Finnish Account...

  5. Bioefficacy of long-lasting insecticidal nets against pyrethroid-resistant populations of Anopheles gambiae s.s. from different malaria transmission zones in Uganda

    OpenAIRE

    Okia, Michael; Ndyomugyenyi, Richard; Kirunda, James; Byaruhanga, Anatol; Adibaku, Seraphine; Lwamafa, Denis K; Kironde, Fred

    2013-01-01

    Background There are major concerns over sustaining the efficacy of current malaria vector control interventions given the rapid spread of resistance, particularly to pyrethroids. This study assessed the bioefficacy of five WHO-recommended long-lasting insecticidal nets (LLINs) against pyrethroid-resistant Anopheles gambiae field populations from Uganda. Methods Adult An. gambiae from Lira, Tororo, Wakiso and Kanungu districts were exposed to permethrin (0.75%) or deltamethrin (0.05%) in stan...

  6. QTL Information Table: 876 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available QSbr3a Resistance or Tolerance Sheath blight resistance sheath blight response rati...ngs (SBRs) RFLP C)Interval F4 Lemont Teqing B RG348 RG944 pha Li, Z., Pinson, S.R.M., Marchetti, M.A., Stans...el, J.W., and Park, W.D. (1995). Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistan

  7. Drug: D00444 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available SYSTEMIC USE A14A ANABOLIC STEROIDS A14AA Androstan derivatives A14AA02 Stanozolol D00444 Stanozolol (JAN/U...D00444 Drug Stanozolol (JAN/USP/INN); Winstrol (TN) C21H32N2O 328.2515 328.4916 D00...eroid receptor androgen receptor [HSA:367] [KO:K08557] Stanozolol [ATC:A14AA02] D00444 Stan

  8. A Case of Stenotic Change from Gastric Candidiasis Managed with Temporary Stent Insertion

    OpenAIRE

    Joo, Moon Kyung; Park, Jong-Jae; Lee, Beom Jae; Kim, Ji Hoon; Yeon, Jong Eun; Kim, Jae Seon; Byun, Kwan Soo; Bak, Young-Tae

    2011-01-01

    Invasive gastric Candida infection in patient with co-morbidity can cause stenotic change if it is developed at anatomically narrowing portion, such as distal antrum, pylorus, or duodenal bulb. However, proper management of benign stenosis by diffuse gastric Candidasis is still under controversy and palliative bypass surgery has several shortcomings because high risk operative group may be included in this case. Palliative placement of self-expandable metallic stent has been settled as a stan...

  9. Quantification of Power Losses of the Interdigitated Metallization of Crystalline Silicon Thin-Film Solar Cells on Glass

    OpenAIRE

    Gress, Peter J.; Sergey Varlamov

    2012-01-01

    The metallization grid pattern is one of the most important design elements for high-efficiency solar cells. This paper presents a model based on the unit cell approach to accurately quantify the power losses of a specialized interdigitated metallization scheme for polycrystalline silicon thin-film solar cells on glass superstrates. The sum of the power losses can be minimized to produce an optimized grid-pattern design for a cell with specific parameters. The model is simulated with the stan...

  10. Prosopis Africana SEEDS (OKPEYE)

    African Journals Online (AJOL)

    User

    Thermal heat diffusivity increased from 2.7 ×10-7 to 4.2 ×10-7m2 s-1 as moisture content increased from 4 to ... of PA at five different moisture content levels (4 to 20%. w.b). 2. ..... [1] Keay, R.W.J., Onochie, C.F.A. and Stan Field, “D.P. I”,. Vol II.

  11. Chemical composition and physical quality characteristics of Ghanaian cocoa beans as affected by pulp pre-conditioning and fermentation

    OpenAIRE

    Afoakwa, Emmanuel Ohene; Quao, Jennifer; Takrama, Jemmy; Budu, Agnes Simpson; Saalia, Firibu Kwesi

    2011-01-01

    Investigations were conducted to evaluate the effects of pod storage (as a means of pulp preconditioning) and fermentation on the chemical composition and physical characteristics of Ghanaian cocoa beans. A 4 × 2 full factorial design with factors as pod storage (0, 7, 14, 21 days) and cocoa treatment (fermented and unfermented) were conducted. Samples were analyzed for their chemical composition (moisture, crude fat, crude protein, ash and carbohydrate content) and mineral content using stan...

  12. Transition Guidelines for A-SMGCS

    OpenAIRE

    Verbeek, René; van Schaik, Frans; Jakobi, Jörn

    2006-01-01

    These Transition Guidelines for Advanced Surface Movement Guidance and Control System (A SMGCS) are intended for use by the stakeholders: airport, ANSP (Air Navigation Service Provider), airline, and industry. The guidelines give an overview of the main STEPS, but not necessarily all steps, for the introduction of and transition to A SMGCS operations at an airport. They address the technical, operational, administrative, training and licensing aspects. The guidelines are no international stan...

  13. Special Operations Forces Interagency Counterterrorism Reference Manual. Third Edition

    Science.gov (United States)

    2013-09-01

    Voluntary Trust Fund. The Mine Action Programme of Afghanistan ( MAPA ) is one of the largest in the world because of the scope of the mine problem...Millennium Challenge Corporation (MCC) www.mcc.gov/ Mine Action Coordination Center of Afghanistan (MACCA) (UN) Mine Action Programme of Afghanistan ( MAPA ...MACCA. Mine Action Coordination Center of Afghani- stan (UN) MAPA . Mine Action Programme of Afghanistan (UN) MARFORCYBER. United States Marine Forces

  14. Graafikatriennaali peapreemia Andres Talile

    Index Scriptorium Estoniae

    2001-01-01

    Tallinna XII graafikatriennaali preemiad: grand prix - Andres Tali; 3 võrdset preemiat - Janne Laine, Leonards Laganovskis, Peeter Laurits; diplomid - Lynne Allen, Lucrecia Urbano, Vladimir Tshaika, Zora Stanè, Endi Poskovic, Päivikki Kallio; EKM preemia - Tif Bitmap; Tallinna linna preemia - Ülle Marks ja Jüri Kass; eripreemiad: - J. Laine (Eesti Pank), Vive Tolli (Paletti Eesti AS), Martins Ratniksh, Juris Boiko, T. Bitmap (Balteco AS). Žürii koosseis

  15. U.S. Policy and Strategy Toward Afghanistan After 2014

    Science.gov (United States)

    2014-10-01

    Central Asia as well. China has invested more than $5 billion in Afghani- stan to date, primarily in the Aynak copper mine and in winning bids to...rebuilding Afghanistan; and 3) Regionalize strategy. The first two pillars of a post-2014 strategy basically require reducing the current U.S. involvement...countries— including Afghanistan—for U.S. interests. The first two pillars to the strategy will not address the last two national interests remaining

  16. Video News Release ALPHA experiment

    CERN Multimedia

    2016-01-01

    Antimatter continues to intrigue physicists due to its apparent absence in the observable universe. Current theory requires that matter and antimatter should have appeared in equal quantities after the Big Bang, but the Stan- dard Model offers no quantitative explanation for the apparent disappearance of half of the universe. It has recently become possible to study trapped atoms1–4 of antihydrogen to search for possible, as yet unobserved, differences in the physical behaviour of matter and antimatter.

  17. ALPHA experiment : limit on the charge of antihydrogen atom

    CERN Multimedia

    2016-01-01

    Antimatter continues to intrigue physicists due to its apparent absence in the observable universe. Current theory requires that matter and antimatter should have appeared in equal quantities after the Big Bang, but the Stan- dard Model offers no quantitative explanation for the apparent disappearance of half of the universe. It has recently become possible to study trapped atoms1–4 of antihydrogen to search for possible, as yet unobserved, differences in the physical behaviour of matter and antimatter.

  18. Development of Subischial Prosthetic Sockets with Vacuum-Assisted Suspension for Highly Active Persons with Transfemoral Amputations

    Science.gov (United States)

    2014-10-01

    International Organization for Stan- dardization, Li-Ion = lithium -ion, VAS = vacuum-assisted sus- pension. *Address all correspondence to Stefania Fatone...both electrical pumps, a lithium -ion (Li-Ion) battery powered a direct current motor, which ran a small capacity pump. Microprocessor circuitry...were deter- mined prior to initiation of the test. At the three weight settings tested for the Harmony P2 and Harmony HD pumps, there were no

  19. Heterogeneous multiscale Monte Carlo simulations for gold nanoparticle radiosensitization.

    Science.gov (United States)

    Martinov, Martin P; Thomson, Rowan M

    2017-02-01

    To introduce the heterogeneous multiscale (HetMS) model for Monte Carlo simulations of gold nanoparticle dose-enhanced radiation therapy (GNPT), a model characterized by its varying levels of detail on different length scales within a single phantom; to apply the HetMS model in two different scenarios relevant for GNPT and to compare computed results with others published. The HetMS model is implemented using an extended version of the EGSnrc user-code egs_chamber; the extended code is tested and verified via comparisons with recently published data from independent GNP simulations. Two distinct scenarios for the HetMS model are then considered: (a) monoenergetic photon beams (20 keV to 1 MeV) incident on a cylinder (1 cm radius, 3 cm length); (b) isotropic point source (brachytherapy source spectra) at the center of a 2.5 cm radius sphere with gold nanoparticles (GNPs) diffusing outwards from the center. Dose enhancement factors (DEFs) are compared for different source energies, depths in phantom, gold concentrations, GNP sizes, and modeling assumptions, as well as with independently published values. Simulation efficiencies are investigated. The HetMS MC simulations account for the competing effects of photon fluence perturbation (due to gold in the scatter media) coupled with enhanced local energy deposition (due to modeling discrete GNPs within subvolumes). DEFs are most sensitive to these effects for the lower source energies, varying with distance from the source; DEFs below unity (i.e., dose decreases, not enhancements) can occur at energies relevant for brachytherapy. For example, in the cylinder scenario, the 20 keV photon source has a DEF of 3.1 near the phantom's surface, decreasing to less than unity by 0.7 cm depth (for 20 mg/g). Compared to discrete modeling of GNPs throughout the gold-containing (treatment) volume, efficiencies are enhanced by up to a factor of 122 with the HetMS approach. For the spherical phantom, DEFs vary with time for diffusion

  20. 致病菌与非致病菌诱导对家蚕抗菌肽基因defensinA/B的表达定量分析%Expression Quantitative Analysis of Bombyx mori Antimicrobial Peptides Genes Bm-defensinA/B Induced by Pthogens and Non-pathogens

    Institute of Scientific and Technical Information of China (English)

    唐芬芬; 杨伟克; 邵榆岚; 杨海; 白兴荣

    2013-01-01

    In this paper,the real-time fluorescent quantitative PCR was applied to analyze the expression of Bm-defensinA/B in fat body after being injected pathogens (Bacillus bombyseptieus) and non-pathogens (Escherichia coli) of Bombyx mori.The results showed that defensinA and defensinB were all up-regulated after injection with B.bombyseptieus and E.coli at 3,9 snd 12 h.Moreover,the expression of defensinBwas higher and faster than that of defensinA.These suggested that Bm-defensins played an important role in B.moriimmune response to against infection by pathogen.%在致病菌(Bacilus bombyseptieus)与非致病菌(Escherichia coli)诱导家蚕后的不同时间,采用实时荧光定量PCR方法,对抗菌肽基因defensinA/B在脂肪体中的表达进行了定量分析.结果表明,在家蚕被注射黑胸败血芽孢杆菌致病菌和非致病菌(大肠杆菌)后的3、9、12 h,检测到脂肪体中Bm-defA/B的转录活性上调,Bm-defB的响应活性高于Bm-defA,Bm-defB对黑胸败血芽孢杆菌致病菌的响应快于Bm-defA,提示Bm-defensins在家蚕对抗细菌侵染的免疫反应中发挥重要的作用.

  1. Newcastle disease virus infection in chicken embryonic fibroblasts but not duck embryonic fibroblasts is associated with elevated host innate immune response.

    Science.gov (United States)

    Kang, Yinfeng; Feng, Minsha; Zhao, Xiaqiong; Dai, Xu; Xiang, Bin; Gao, Pei; Li, Yulian; Li, Yanling; Ren, Tao

    2016-03-15

    Chickens and ducks are major hosts of Newcastle disease virus (NDV) with distinct responses to infection. However, whereas ducks are generally asymptomatic or exhibit only mild symptoms following NDV infection and are thus regarded as potential long-term reservoirs of the virus, chickens exhibit severe clinical lesions, transient infections and even death due to NDV infection. These differences may in part result from the host innate immune response to NDV infection. To better understand the host innate immune response to NDV infection in avian species, by using the quantitative real-time polymerase chain reaction method we examined the messenger RNA expression levels of immune-related genes in chicken embryonic fibroblasts (CEFs) and duck embryonic fibroblasts (DEFs) when infected with NDV of different pathogenicities. Gene expression profiles showed that the expression of IL-1beta, TNF-α-like factor (LITAF) and interferon (IFN)-beta was upregulated in both CEFs and DEFs infected with SS-10 and NH-10 viruses or treated with polyinosinic:polycytidylic acid [poly(I:C)], as well as that expression levels were greater in CEFs than in DEFs. The expression of TLR3, TLR7, IL-6, IFN-alpha, IFN-gamma, MHC-I and MHC-II, except for IL-8, were also greater in CEFs than in DEFs in response to infection to both viruses or treatment with poly(I:C). However, unlike moderate virulent NH-10, highly virulent SS-10 induced greater pattern recognition receptors and cytokines, except for IFNs, in CEFs and DEFs. Results show distinct expression patterns of cytokines, Toll-like receptors and IFNs associated with inflammatory immune responses to NDV between species and by virulence.

  2. Genetically modified parthenocarpic eggplants: improved fruit productivity under both greenhouse and open field cultivation.

    Directory of Open Access Journals (Sweden)

    Pandolfini Tiziana

    2002-04-01

    Full Text Available Abstract Background Parthenocarpy, or fruit development in the absence of fertilization, has been genetically engineered in eggplant and in other horticultural species by using the DefH9-iaaM gene. The iaaM gene codes for tryptophan monoxygenase and confers auxin synthesis, while the DefH9 controlling regions drive expression of the gene specifically in the ovules and placenta. A previous greenhouse trial for winter production of genetically engineered (GM parthenocarpic eggplants demonstrated a significant increase (an average of 33% increase in fruit production concomitant with a reduction in cultivation costs. Results GM parthenocarpic eggplants have been evaluated in three field trials. Two greenhouse spring trials have shown that these plants outyielded the corresponding untransformed genotypes, while a summer trial has shown that improved fruit productivity in GM eggplants can also be achieved in open field cultivation. Since the fruits were always seedless, the quality of GM eggplant fruits was improved as well. RT-PCR analysis demonstrated that the DefH9-iaaM gene is expressed during late stages of fruit development. Conclusions The DefH9-iaaM parthenocarpic gene is a biotechnological tool that enhances the agronomic value of all eggplant genotypes tested. The main advantages of DefH9-iaaM eggplants are: i improved fruit productivity (at least 30–35% under both greenhouse and open field cultivation; ii production of good quality (marketable fruits during different types of cultivation; iii seedless fruit with improved quality. Such advantages have been achieved without the use of either male or female sterility genes.

  3. Global longitudinal strain: a useful everyday measurement?

    Directory of Open Access Journals (Sweden)

    A King

    2016-10-01

    Full Text Available Herceptin (Trastuzumab is a widely used and effective drug for the treatment of Her2+ breast cancer but its cardiotoxic side effects require regular monitoring by echocardiography. A 10% reduction in left ventricular ejection fraction can lead to suspension of treatment and therefore has significant implications for patient prognosis in terms of cardiac and cancer outcomes. Assessment of LV function by conventional 2D biplane method of discs (2DEF has limitations in accuracy and reproducibility. Global longitudinal strain (GLS is becoming more widely available and user friendly. It has been shown to demonstrate myocardial damage earlier in treatment than 2DEF, allowing the option of pharmacological intervention at a pre-clinical stage and preventing the interruption of Herceptin. This study compares the reproducibility of GLS with that of 2DEF in a routine clinical environment. Fifty echocardiograms performed on female patients undergoing Herceptin treatment were used to measure both 2DEF and GLS within the recommended standard appointment time of 40 min. The data were re-measured (blind by the same operator a minimum of 14 days later to determine intra-operator variation. These data were also measured by a second operator (blind, to assess inter-operator variation. Analysis by direct comparison, intra-class correlation (ICC, coefficient of variation (CV and Bland–Altman plots demonstrated that GLS is a more reproducible measurement than 2DEF. This is important to prevent clinical decisions being erroneously based on variation in operator measurement. The investigation also shows that with advances in machine software this is a practical addition to routine assessment rather than merely a research tool.

  4. Maternal n-3 polyunsaturated fatty acid deprivation during pregnancy and lactation affects neurogenesis and apoptosis in adult offspring: associated with DNA methylation of brain-derived neurotrophic factor transcripts.

    Science.gov (United States)

    Fan, Chaonan; Fu, Huicong; Dong, Hua; Lu, Yuanyuan; Lu, Yanfei; Qi, Kemin

    2016-09-01

    In this study, we hypothesized that n-3 polyunsaturated fatty acid (PUFA) deficiency during pregnancy and lactation will make a lasting impact on brain neurogenesis and apoptosis of the adult offspring and that these harmful effects cannot be reversed by n-3 PUFA supplementation after weaning. Moreover, the underlying mechanisms may be attributable to the epigenetic changes of brain-derived neurotrophic factor (BDNF). C57BL/6J female mice were fed with n-3 PUFA-deficient diet (n-3 def) or n-3 PUFA-adequate diet (n-3 adq) throughout pregnancy and lactation. At postnatal 21 days, equal numbers of male pups from both groups were fed the opposite diet, and the remaining male pups were fed with the same diets as their mothers until 3 months of age. Feeding the n-3 adq diet to pups from the maternal n-3 def group significantly increased the n-3 PUFA concentration but did not change expressions of calretinin, Bcl2, and Bax in the hippocampus. Feeding the n-3 def diet to pups from the maternal n-3 adq group significantly reduced the n-3 PUFA concentration but did not reduce expressions of calretinin and Bcl2. Similarly, BDNF levels, especially mRNA expressions of BDNF transcripts IV and IX, were also reduced by maternal n-3 def and not reversed by n-3 PUFA supplementation after weaning. The decrease in BDNF expression by maternal n-3 def diet was associated with greater DNA methylation at special CpG sites. These results suggested that the maternal n-3 PUFA deficiency during pregnancy and lactation imprints long-term changes of brain development in adult offspring.

  5. Role of PON2 in innate immune response in an acute infection model.

    Science.gov (United States)

    Devarajan, Asokan; Bourquard, Noam; Grijalva, Victor R; Gao, Feng; Ganapathy, Ekambaram; Verma, Jitendra; Reddy, Srinivasa T

    2013-11-01

    N-(3-oxododecanoyl)-l-homoserine lactone (3OC(12)-HSL) is a quorum-sensing molecule produced by gram-negative microbial pathogens such as Pseudomonas aeruginosa (PAO1). 3OC(12)-HSL is involved in the regulation of bacterial virulence factors and also alters the function of the host immune cells. Others and we have previously shown that paraoxonase 2 (PON2), a member of the paraoxonase gene family expressed in immune cells, hydrolyzes 3OC(12)-HSL. In this study, we examined i) whether macrophage PON2 participates in 3OC(12)-HSL hydrolysis, ii) the effect of PON2 deficiency in acute PAO1 infection in mice and iii) the effect of 3OC(12)-HSL on PON2 deficient (PON2-def) macrophages. When compared to wild type macrophages, both intact cells and membrane-enriched protein lysates obtained from PON2-def macrophages show a marked impairment in their ability to hydrolyze 3OC(12)-HSL. PON2 expression (message and protein) is not altered in response to 3OC(12)-HSL in macrophages. 3OC(12)-HSL treated PON2-def macrophages showed i) an increase in ER stress and oxidative stress, ii) defective phosphatidylinositol 3-kinase (PI3 kinase)/AKT activation, and iii) reduced phagocytosis function. Moreover, the nitration to phosphorylation ratio of Tyr458 in p85 protein, the regulatory subunit of PI3-kinase that has been correlated with the phagocytosis function of macrophages, was increased in PON2-def macrophages. Antioxidant treatment reversed the effects of PON2 deficiency in macrophage phagocytosis function. Furthermore, following administration of 1.6 × 10(7) CFU of PAO1, bacterial clearance was significantly reduced in the lungs (5.7 fold), liver (2.5 fold), and spleen (14.8 fold) of PON2-def mice when compared to wild type mice. Our results suggest that PON2 plays an important role in innate immune defense against PAO1 infection.

  6. DFT Simulation of Structural and Optical Properties of 9-Aminoacridine Half-Sandwich Ru(II), Rh(III), and Ir(III) Antitumoral Complexes and Their Interaction with DNA.

    Science.gov (United States)

    Cerón-Carrasco, José Pedro; Ruiz, José; Vicente, Consuelo; de Haro, Concepción; Bautista, Delia; Zúñiga, José; Requena, Alberto

    2017-08-08

    In this work, we use DFT-based methods to simulate the chemical structures, optical properties, and interaction with DNA of a recently synthesized chelated C^N 9-aminoacridine arene Ru(II) anticancer agent and two new closely related Rh(III) and Ir(III) complexes using DFT-based methods. Four chemical models and a number of theoretical approaches, which representatively include the PBE0, B97D, ωB97X, ωB97X-D, M06, and M06-L density functionals and the LANL2DZ, def2-SVP, and def2-TZVP basis sets, are tested. The best overall accuracy/cost performance for the optimization process is reached at the ωB97X-D/def2-SVP and M06/def2-SVP levels of theory. Inclusion of explicit solvent molecules (CHCl3) further refines the geometry, while taking into account the crystal network gives no significant improvements of the computed bond distances and angles. The analysis of the excited states reveals that the M06 level matches better the experimental absorption spectra, compared to ωB97X-D. The use of the M06/def2-SVP approach is therefore a well-balanced method to study theoretically the bioactivity of this type of antitumoral complexes, so we couple this TD-DFT approach to molecular dynamics simulations in order to assess their reactivity with DNA. The reported results demonstrate that these drugs could be used to inject electrons into DNA, which might broaden their applications in photoactivated chemotherapy and as new materials for DNA-based electrochemical nanodevices.

  7. SU-E-T-279: Dose Enhancement Effect Due to Cerium Oxide Nanoparticles Employed as Radiation Protectants

    Energy Technology Data Exchange (ETDEWEB)

    Ouyang, Z; Altundal, Y; Sajo, E [Univ Massachusetts Lowell, Lowell, MA (United States); Ngwa, W [Univ Massachusetts Lowell, Lowell, MA (United States); Brigham and Women’s Hospital, Dana Farber Cancer Institute, Harvard Medical, Boston, MA (United States)

    2015-06-15

    Purpose: The goal of radiotherapy is to maximize radiation dose to diseased cells while minimizing radiation damage to normal tissues. In order to minimize damage to normal tissues, cerium oxide nanoparticles (nanoceria) are currently considered as a radioprotectant. However, some studies have reported concerns that nanoceria can also lead to radiotherapy dose enhancement due to the high atomic number of cerium, especially when used in conjunction with kV energy and brachytherapy sources. In this study, this concern is investigated to determine if the concentrations of nanoceria employed in in-vivo studies to confer radioprotection can engender a significant dose enhancement. Methods: Radiation with energies ranging from 50kVp to 140kVp is investigated in this work along with brachytherapy sources Pd-103 and I-125. A previously established theoretical model is used to calculate the dose enhancement factor (DEF). In this model, each cell is assumed to be a voxel of size (10 µm, 10 µm, 10 µm) with nanoceria homogeneously distributed among them. Electron energy loss formula of Cole is used to calculate energy (and hence dose) deposited by photoelectrons and Auger electrons in each tissue voxel due to irradiation of nanoceria. The DEF is defined as the ratio of the dose with and without nanoparticles. Results: DEF calculation results are smaller than 1.02 with dosages of nanoceria smaller than 0.645 mg/g, which is shown to be sufficiently protective by some previous in-vitro and in-vivo experiments. The brachytherapy sources show higher DEF’s than kVp radiations. DEF peaks are consistent with K shell and L shell energies of cerium, 40 keV and 6 keV, respectively. Conclusion: The results show that for sufficiently radioprotective concentrations of nanoceria, there will be minimal DEF when used in conjunction with clinically applicable kV energy radiotherapy sources or brachytherapy sources.

  8. Floral extract ofTecoma stans:A potent inhibitor of gentamicin-induced nephrotoxicityin vivo

    Institute of Scientific and Technical Information of China (English)

    Raju S; Kavimani S; Uma Maheshwara rao V; Sreeramulu Reddy K; Vasanth Kumar G

    2011-01-01

    Objective:To highlight the nephroprotective activity of ethyl acetate extract of dried flowers ofTecoma stans for its protective effects on gentamicin-induced nephrotoxicity in albino rats. Methods:For studying acute toxicity study, single oral dose of5 000 mg ethyl acetate floral extract/kg body weight was administered to albino rats (five females, five males). Nephrotoxicity was induced in albino rats by intraperitoneal administration of gentamicin80 mg/kg/day for eight days. Effect of concurrent administration of ethyl acetate floral extract of Tecoma stans at a dose of 100, 200 and 300 mg/kg/day given by oral route was determined using serumcreatinine, serum uric acid, blood urea nitrogen and serum urea as indicators of kidney damage. The study groups contained six rats in each group. As nephrotoxicity of gentamicin is known to involve induction of oxidative stress,in vitro antioxidant activity and free radical-scavenging activity of this extract was also evaluated.Results:For acute toxicity testing both female and male rats administered with the extract at a dose of5 000mg/kg. The results showed no toxicity in terms of general behavior change, mortality, or change in gross appearance of internal organs (LD50 > 5 000 mg/kg). It was observed that the ethyl acetate floral extract ofTecoma stans significantly protected rat kidneys from gentamicin-induced histopathological changes. Gentamicin-induced glomerular congestion, peritubular and blood vessel congestion, epithelial desquamation, accumulation of inflammatory cells and necrosis of the kidney cells were found to be reduced in the groups receiving the ethyl acetate floral extract ofTecoma stans along with gentamicin in a dose dependent manner. The floral extract also reduced the gentamicin-induced increase in serum creatinine, serum uric acid, blood urea nitrogen and serum urea levels (P>0.01).Conclusions:The present study indicates a very important role of reactive oxygen species (ROS)and the relation to

  9. CERN Scientific Book Fair 2013

    CERN Document Server

    CERN Library

    2013-01-01

    The CERN Bookshop and CERN Library invite you to attend the 2013 CERN Book Fair, a two-day scientific event offering you the opportunity to meet key publishers and to browse and purchase books at significant discounts.   Key publishers will present a selection of titles in physics, technology, mathematics, engineering, computing and popular science. You are welcome to come along and meet the publishers’ representatives or simply have a look at the books on sale. The fair will take place in the Main Building (Bldg. 500) on the ground floor near Restaurant 1 on Monday 9 and Tuesday 10 September. Participating or represented publishers include: Oxford University Press, Princeton University Press, Springer, Wiley, and World Scientific-Imperial College Press. Fair opening times:  - Monday 9 September 9:00 - 18:00  - Tuesday 10 September 9:00 - 18:00

  10. 21ST International Symposium on Rarefied Gas Dynamics. Marseille (France) 26-31 July 1998. Book of Abstracts: Volume III, Special Session; Molecular Beams.

    Science.gov (United States)

    1998-07-30

    8217 1 Dipartimento di Chimica dell’UniversitY, Perugia, Italy 2 Instituto Pluridisciplinar, Universidad Complutense , Madrid , Spain 1 Introduction...Jim~nez, A. GonzAlez Urefia Unidad de Laseres y Haces Moleculares. Universidad Complutense de Madrid . Spain. The spectroscopy and dynamics of We have...continued) 17:00 - 18:30 POSTER SESSION MB - P Chair: A. Gonzalez-Urena ( Madrid ) Observation and Spectroscopy of Metastable 04 D.S. Peterka, M. Ahmed

  11. Boundary Layer Parameterization for a Global Spectral Model

    Science.gov (United States)

    1991-01-27

    submitting to a refereed journal. An additional study was conducted to examine how the relative humidity field evolves in the modelled daytime boundary...zo.\\MNz0H=10 15.’- z0.NI0H=100 6.00 9.00 12.00 15.00 18.00 Tine ( DrC ) Figure 4. As Figure 2 for the skin temperature. 136 15000 EzOk1500O

  12. 15 CFR Appendix A to Part 950 - Schedule of User Fees for Access to NOAA Environmental Data

    Science.gov (United States)

    2010-01-01

    ....00 2.00 Online In-Situ Digital Data 10.00 6.00 Data Poster 18.00 18.00 Shipping Service: For orders... film 13.00 13.00 Satellite Image Product 80.00 82.00 Offline Satellite Digital Data (average unit size is 1.2 gigabytes) 115.00 Offline Radar Digital Data (Level II) (average unit size is 4 gigabytes)...

  13. 75 FR 81110 - Schedule of Fees for Access to NOAA Environmental Data, Information, and Related Products and...

    Science.gov (United States)

    2010-12-27

    ... products is growing exponentially, both in size and sophistication. Users have the ability to access the............ 6.00 6.00 Data Poster 18.00 18.00 Shipping Service for orders totaling less than 3.00 3.00 $50.00... Satellite Image Product 82.00 83.00 Offline Satellite Digital Data (average unit size is 1.2...

  14. Data of evolutionary structure change: 1A4SD-2BJKA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 86 1.00 18.34 C ATOM 11792 CA CYS D 50 38.824 18.347 -23.577 1.00 20.12 C ... VAL CA 5.68 3.82 LEU CA 3.83 CYS CA -map> P...ine> -map> GLY VAL VAL GLU SER PRO ALA SER PRO ASN LEU CA ... CA 3.78 GLY CA -map> LEU CA 406 -map>

  15. PENGARUH EKSTRAK DAUN SIRSAK Annona muricata L TERHADAP PERIODE MENGHISAP DARAH NYAMUK Aedes aegypti

    OpenAIRE

    Pangadongan, Acice T.

    2016-01-01

    Telah dilakukan penelitian tentang pengaruh ekstrak daun sirsak Annona muricata L terhadap periode menghisap darah nyamuk Aedes aegypti. Penelitian ini bersifat eksperimental menggunakan ekstrak daun sirsak dalam bentuk spray konsentrasi 3,7%, 7,5%, 15% dan 30% yang disemprotkan ke tangan. Selanjutnya dimasukkan ke dalam kandang yang telah berisi sampel uji nyamuk betina sebanyak 20 ekor, pengamatan dimulai pukul 08.00-18.00 WITA. Aktivitas ekstrak dihitung dengan cara menghitung jumlah nyam...

  16. An OTP-Based Mechanism for Defending Application Layer DDoS Attacks%基于动态口令的应用层DDoS攻击防御方案

    Institute of Scientific and Technical Information of China (English)

    叶晰; 温武少; 叶依如

    2012-01-01

    In this paper, we present the design and implementation of OTP-DEF, a kernel extension to protect web servers against application layer DDoS attacks. First of all, according to the load of web server, an OTP-DEF web server should fall into one of three following modes: normal, suspected attack or confirmed attack mode, and the OTP-DEF authentication mechanism shall only be activated when web server is in suspected attack mode. Secondly, we use OTP as our puzzle, which can automatically change at the certain time interval. It makes our proposal can defend copy attacks, replay attacks and Brute-Force Attack. Thirdly, OTP-DEF uses an intermediate stage to identify the IP addresses that ignore the test, and persistently bombard the server with requests despite repeated failures at solving the puzzles. Once these machines are identified, OTP-DEF blocks their requests, turns the tests off, and allows access to legitimate users who are unable or unwilling to solve tests. Finally, OTP-DEF requires no modifications to client software.%研究和设计了使用动态口令技术来保护服务器抵御DDoS攻击的OTP-DEF方案.首先,方案可根据服务器工作负载的不同,分别处于正常、疑似受攻击或确认受攻击3种工作模式之下,而基于动态口令的认证方案只在疑似受攻击工作模式下起作用.其次,由于动态口令会自动变化,故方案可抵御复制、重放和暴力破解攻击.第三,通过记录那些不解决难题并不断发送请求的IP地址来识别客户端是否为攻击者,一旦所有攻击者被识别出来后,OTP-DEF屏蔽其IP地址并停止发布难题,以便正常用户能方便地使用服务.最后,只需在服务器端实施和部署,客户端无需做任何更改.

  17. Arbitrary Angular Momentum Electron Repulsion Integrals with Graphical Processing Units: Application to the Resolution of Identity Hartree-Fock Method.

    Science.gov (United States)

    Kalinowski, Jaroslaw; Wennmohs, Frank; Neese, Frank

    2017-07-11

    A resolution of identity based implementation of the Hartree-Fock method on graphical processing units (GPUs) is presented that is capable of handling basis functions with arbitrary angular momentum. For practical reasons, only functions up to (ff|f) angular momentum are presently calculated on the GPU, thus leaving the calculation of higher angular momenta integrals on the CPU of the hybrid CPU-GPU environment. Speedups of up to a factor of 30 are demonstrated relative to state-of-the-art serial and parallel CPU implementations. Benchmark calculations with over 3500 contracted basis functions (def2-SVP or def2-TZVP basis sets) are reported. The presented implementation supports all devices with OpenCL support and is capable of utilizing multiple GPU cards over either MPI or OpenCL itself.

  18. Adult vitamin D deficiency exacerbates impairments caused by social stress in BALB/c and C57BL/6 mice

    DEFF Research Database (Denmark)

    Groves, Natalie J; Zhou, Mei; Jhaveri, Dhanisha J

    2017-01-01

    ) or Social Defeat (DEF). SEP mice were placed two per cage with a perforated Plexiglas divider, whereas the DEF mice underwent 10days of social defeat prior to behavioural testing. We found that AVD-deficient mice were more vulnerable to the effects of social stress using a social avoidance test......Vitamin D deficiency is prevalent in adults throughout the world. Epidemiological studies have shown significant associations between vitamin D deficiency and an increased risk of various neuropsychiatric and neurodegenerative disorders, such as schizophrenia, depression, Alzheimer's disease...... and cognitive impairment. However, studies based on observational epidemiology cannot address questions of causality; they cannot determine if vitamin D deficiency is a causal factor leading to the adverse health outcome. The main aim of this study was to determine if AVD deficiency would exacerbate the effects...

  19. Solvent-induced assembly of two helical Eu(III) metal-organic frameworks and fluorescence sensing activities towards nitrobenzene and Cu2+ ions

    Science.gov (United States)

    Ma, Ranran; Chen, Zhiwei; Wang, Suna; Yao, Qingxia; Li, Yunwu; Lu, Jing; Li, Dacheng; Dou, Jianmin

    2017-08-01

    Two helical Eu(III) metal-organic frameworks, namely, {[Eu(L)(DMF)(H2O)]·0.5DMF}n (1) and [Eu(L)(DEF)(H2O)]n (2) (H3L=3,5-bis(2-carboxylphenoxy)benzoic acid, DMF=N,N-dimethylformamide, DEF=N,N-diethylformamide), have been solvothermally synthesized in different solvents, respectively. Both complexes possess helical structures through the connectivity of Eu atoms and phenolic-oxygen containing branches of the flexible multicarboxylate ligand. Based on different helices, these two complexes exhibited hexagonal and tetragonal channels, respectively. Both complexes possess (3,6)-connected (4.62)2(42.610.83) topology but with different long Schlafli symbol. The solvent plays an important role in the formation of the final frameworks. Both complexes can sensitively and selectively detect nitrobenzene and Cu2+ ions.

  20. A novel high-performance high-frequency SOI MESFET by the damped electric field

    Science.gov (United States)

    Orouji, Ali A.; Khayatian, Ahmad; Keshavarzi, Parviz

    2016-06-01

    In this paper, we introduce a novel silicon-on-insulator (SOI) metal-semiconductor field-effect-transistor (MESFET) using the damped electric field (DEF). The proposed structure is geometrically symmetric and compatible with common SOI CMOS fabrication processes. It has two additional oxide regions under the side gates in order to improve DC and RF characteristics of the DEF structure due to changes in the electrical potential, the electrical field distributions, and rearrangement of the charge carriers. Improvement of device performance is investigated by two-dimensional and two-carrier simulation of fundamental parameters such as breakdown voltage (VBR), drain current (ID), output power density (Pmax), transconductance (gm), gate-drain and gate-source capacitances, cut-off frequency (fT), unilateral power gain (U), current gain (h21), maximum available gain (MAG), and minimum noise figure (Fmin). The results show that proposed structure operates with higher performances in comparison with the similar conventional SOI structure.

  1. Ternary complex formation between the MADS-box proteins SQUAMOSA, DEFICIENS and GLOBOSA is involved in the control of floral architecture in Antirrhinum majus.

    Science.gov (United States)

    Egea-Cortines, M; Saedler, H; Sommer, H

    1999-10-01

    In Antirrhinum, floral meristems are established by meristem identity genes. Floral meristems give rise to floral organs in whorls, with their identity established by combinatorial activities of organ identity genes. Double mutants of the floral meristem identity gene SQUAMOSA and organ identity genes DEFICIENS or GLOBOSA produce flowers in which whorled patterning is partially lost. In yeast, SQUA, DEF and GLO proteins form ternary complexes via their C-termini, which in gel-shift assays show increased DNA binding to CArG motifs compared with DEF/GLO heterodimers or SQUA/SQUA homodimers. Formation of ternary complexes by plant MADS-box factors increases the complexity of their regulatory functions and might be the molecular basis for establishment of whorled phyllotaxis and combinatorial interactions of floral organ identity genes.

  2. Dvadasham (Dodeca Edge Filter for Impulse Noise, Gaussian Noise, Quantum Noise Reduction in Images (A Generic Image Filter

    Directory of Open Access Journals (Sweden)

    Naveen R Chanukotimath

    2013-07-01

    Full Text Available All image processing techniques need to extract meaningful information from images. However, the noise generated during image acquisition and transmission degrades the human interpretation, or computer-aided analysis of these images. Therefore, denoising should be performed to improve the image quality for more accurate analysis and diagnosis, So we thought of designing a generic image filter that can be applicable to remove Impulse noise, Gaussian noise, Quantum noise. In this paper we propose a novel image denoising technique Dvadasham (Dodeca Edge Filter (DEF. We applied this filter on various images, obtained the results by measuring parameters like Standard Deviation, Homogeneity and compared it with the results of existing Fuzzy Filter. The results obtained with DEF are quite promising than Fuzzy Filter.

  3. Structural diversity of alkaline-earth 2,5-thiophenedicarboxylates

    Science.gov (United States)

    Balendra; Ramanan, Arunachalam

    2017-03-01

    Exploration of the structural landscape of the system containing divalent alkaline-earth metal ion (Mg, Ca and Sr) with the rigid 2,5-thiophenedicarboxylic acid (TDC) under varying solvothermal condition (DMF, DMA and DEF) yielded five new crystals: [Mg(TDC) (DEF)2(H2O)1/2] (1), [Ca(TDC) (DMA)] (2), [Ca(TDC) (DMA) (H2O)] (3), [Sr(TDC) (DMA)] (4) and [Sr(TDC) (DMA) (H2O)] (5) and two known solids. Single crystal structures of all the solids are characteristic of extended coordination interaction between metal and carboxylate ions. While the smaller magnesium ion crystallized into a 2D coordination polymer, the larger calcium and strontium compounds resulted into the growth of 3D metal organic frameworks. All the solids show blue emission arising from intra ligand charge transfer.

  4. Dynamic event Tress applied to sequences Full Spectrum LOCA. Calculating the frequency of exceedance of damage by integrated Safety Analysis Methodology; Arboles de sucesos dinamicos aplicados a secuencias Full Spectrum LOCA. Calculo de la frequencia de excedencia del dano mediante la metodologia Analisis Integrados de Seguridad (ISA)

    Energy Technology Data Exchange (ETDEWEB)

    Gomez-Magan, J. J.; Fernandez, I.; Gil, J.; Marrao, H.; Queral, C.; Gonzalez-Cadelo, J.; Montero-Mayorga, J.; Rivas, J.; Ibane-Llano, C.; Izquierdo, J. M.; Sanchez-Perea, M.; Melendez, E.; Hortal, J.

    2013-09-01

    The Integrated Safety Analysis (ISA) methodology, developed by the Spanish Nuclear Safety council (CSN), has been applied to obtain the dynamic Event Trees (DETs) for full spectrum Loss of Coolant Accidents (LOCAs) of a Westinghouse 3-loop PWR plant. The purpose of this ISA application is to obtain the Damage Exceedance Frequency (DEF) for the LOCA Event Tree by taking into account the uncertainties in the break area and the operator actuation time needed to cool down and de pressurize reactor coolant system by means of steam generator. Simulations are performed with SCAIS, a software tool which includes a dynamic coupling with MAAP thermal hydraulic code. The results show the capability of the ISA methodology to obtain the DEF taking into account the time uncertainty in human actions. (Author)

  5. Decreased gastrointestinal toxicity associated with a novel capecitabine schedule (7 days on and 7 days off): a systematic review

    OpenAIRE

    Cadoo, Karen A; Gajria, Devika; Suh, Emily; Patil, Sujata; Theodoulou, Maria; Norton, Larry; Hudis, Clifford A.; Traina, Tiffany A

    2016-01-01

    Capecitabine is widely used in the management of metastatic breast cancer; however, drug delivery is limited by gastrointestinal and other toxicity. We employed mathematical modeling to rationally design an optimized dose and schedule for capecitabine of 2,000?mg twice daily, flat dosing, 7 days on, 7 days off. Preclinical data suggested increased efficacy and tolerability with this novel dosing, and three early-phase clinical trials have suggested a favorable toxicity profile. To further def...

  6. Dollar Summary of Prime Contract Awards by State, Place, and Contractor, FY84, Part 1 (Adamsville, Alabama-Santa Clara, California).

    Science.gov (United States)

    1984-01-01

    INC 167 167 C P I MACHINING CO INC 2,761 2,761 CALCOMP CORPORATION 55 55 CALIFORNIA CMPTR PRDTS INC 201 49 97 30 25 CARTWRIGHT ENGINEERING INC 6,000...UNITED MEDIA INC 28 28 UNITED STATES DEPARTMENT DEF 43 43 WALTER DORWIN TEAGUE INC 254 254 YEAGER MANUFACTURING CORF 36 36 TOTAL - ANAHEIM 1,020,504...POMONA T & T INDUSTRIES 57 57 WALTER DORWIN TEAGUE INC 2,013 2,013

  7. Annexin II-Dependent Mechanism of Breast Cancer Progression

    Science.gov (United States)

    2010-11-01

    antisenseproducts.Whereas the cellularuptake of antisense ODNs may be enhanced through various cationic lipids , most of the cationic lipids are cytotoxic, and the...44]. By inhibiting COX-2, AA m etabolism is mitigated in order to stimulate the production of ceramide , a mediator of apoptosis [3]. INDUCIBLE...al. Mice def icient in tumor necrosis factor-alpha are resistant to skin carcinogenesis. Nat Med 1999; 5 (7): 828-31. [28] Siegall CB, S chwab G

  8. An exploration of the basis for patient complaints about the oldness of magazines in practice waiting rooms: cohort study

    OpenAIRE

    Arroll, Bruce; Alrutz, Stowe; Moyes, Simon

    2014-01-01

    Objective To explore the basis for patient complaints about the oldness of most magazines in practice waiting rooms. Design Cohort study. Setting Waiting room of a general practice in Auckland, New Zealand. Participants 87 magazines stacked into three mixed piles and placed in the waiting room: this included non-gossipy magazines (Time magazine, the Economist, Australian Women’s Weekly, National Geographic, BBC History) and gossipy ones (not identified for fear of litigation). Gossipy was def...

  9. Risk analysis of the delayed ettringite formation in pile caps foundation in the metropolitan region of Recife - PE - Brasil

    Directory of Open Access Journals (Sweden)

    I. F. Torres

    Full Text Available ABSTRACT Currently, there is an awareness that is critical to assess the durability characteristics of concrete with as much attention as the mechanical properties. The durability of concrete structures can often be affected by chemical attacks, jeopardizing its performance and security. When concrete is subjected to high temperature at early ages, many physical and chemical changes in hardened concrete may occur. It iswidely accepted that concrete subjected to these conditions of temperature and exposed to moisture is prone to cracking due to Delayed Ettringite Formation (DEF. This work aims at providing a DEF risk analysis on foundation pile caps at the Metropolitan Region of Recife - PE. Temperature rise measurement was performed in situ at 5 different caps through datalogger and thermocouples equipments. Furthermore, the Duggan test was performed in order to assess the level of expansion of 3 cements studied: X (CP II E 40, Y (CP II F 32 and Z (CP V ARI RS. Simultaneously, the chemical compositions of these cements and their respective clinkers were quantified by analysis of X-ray fluorescence (XRF. The cement X (CP II E 40 showed the chemical characteristics favoring with more intensity DEF and, as a result, higher level of expansion in the test Duggan. It is noteworthy that incorporation of metakaolin (8% and 16% and silica fume (5% and 10% showed mitigating potential of expansions. It is important to point out that all factors related to thermal properties and chemical composition of the concrete used in the region converge to a condition of ideal susceptibility for triggering DEF. Therefore, it is essential at least minimum and basic requirements in the design specification in order to avoid high temperatures in the massive concrete elements, preventing them from delayed ettringite formation.

  10. Safety and Outcomes of Open-Label Deferasirox Iron Chelation Therapy for Mucormycosis▿

    OpenAIRE

    2009-01-01

    We sought to describe the safety profile of open-label, adjunctive deferasirox iron chelation therapy in eight patients with biopsy-proven mucormycosis. Deferasirox was administered for an average of 14 days (range, 7 to 21) at 5 to 20 mg/kg of body weight/day. The only adverse effects attributable to deferasirox were rashes in two patients. Deferasirox treatment was not associated with changes in renal or liver function, complete blood count, or transplant immunosuppressive levels. Thus, def...

  11. France, tourism

    OpenAIRE

    Jean-Pierre, Philippe; Perrain, David

    2016-01-01

    International audience; This encyclopedia is the most comprehensive and updated source of reference in tourism research and practice. It covers both traditional and emerging concepts and terms and is fully international in its scope. More than 700 contributions of 766 internationally renowned experts from 113 countries provide a definitive access to the knowledge in the field of tourism, hospitality, recreation and related fields. All actors in this field will find reliable and up to date def...

  12. Plasticity In High Temperature Materials: Tantalum and Monazite

    Science.gov (United States)

    2014-03-12

    centered cu- bic tantalum, the methodology also demonstrated a relationship between dislocation mean free path length and GND density. A framework to...c) _ [110] x1 x2 F undeformed def orm ed ω3 x 1’ x 2’ (b) φ1=54.7° (110) [001] [110] _ x1 x2 S(1) S(2) S(3) _ _ (a) [110] [001] [110] (111)[101

  13. Disease: H00552 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H00552 Glycerol kinase deficiency (GKD), including: Hyperglycerolemia; Chromosome X...p21 deletion syndrome; X-linked Addison disease (AHX); Duchenne muscular dystrophy (DMD) Glycerol kinase def... GKD is an Xp21 contiguous gene syndrome involving the glycerol kinase locus together with X-linked Addison disease... (AHX) or Duchenne muscular dystrophy (DMD) loci or both. Inherited metabolic disease hsa00561(2710) ...JK, Duran M, Beemer FA, Poll-The BT Isolated and contiguous glycerol kinase gene

  14. Muscle-derived Decellularised Extracellular Matrix Improves Functional Recovery in a Rat Latissimus Dorsi Muscle Defect Model

    Science.gov (United States)

    2013-12-01

    Piccolo R, et al. Experimental abdominal wall defect repaired with acellular matrix. Pediatr Surg Int 2002;18:327e31. 28. Willett NJ, Li MT, Uhrig BA...matrix (M-ECM). Methods: Ten percent of the mass of the latissimus dorsi (LD) was excised. Three groups were examined: 1) no repair of defect (DEF), 2...to develop therapies for ex- tremity10,11 or abdominal muscle repair.16 For the devel- opment of potential therapies for craniofacial muscle repair

  15. Quantum Rod Emission Coupled to Plasmonic Lattice Resonances: A Collective Directional Source of Polarized Light

    CERN Document Server

    Rodriguez, S R K; Verschuuren, M A; Gomes, R; Lambert, K; De Geyter, B; Hassinen, A; Van Thourhout, D; Hens, Z; Rivas, J Gomez

    2013-01-01

    We demonstrate that an array of optical antennas may render a thin layer of randomly oriented semiconductor nanocrystals into an enhanced and highly directional source of polarized light. The array sustains collective plasmonic lattice resonances which are in spectral overlap with the emission of the nanocrystals over narrow angular regions. Consequently, di?fferent photon energies of visible light are enhanced and beamed into def?nite directions.

  16. Airpower in Hybrid War: Ethical Implications for the Joint Force Commander

    Science.gov (United States)

    2014-05-15

    building housing three adversaries designated as a high value target. However, in this scenario ten children also live in the building. One targeting option...makes bridging tactics and strategy extremely difficult. The character of hybrid war will likely contain an enemy with an intangible center of gravity ...Law” def. 1, accessed 15 May 2014. 4 Kevin M. Brew , “Legal Basis for use of Force and Law of Armed Conflict,” U.S. Naval War College, Joint

  17. 2D Articulatory Velum Modeling Applied to Copy Synthesis of Sentences Containing Nasal Phonemes

    OpenAIRE

    Laprie, Yves; Elie, Benjamin; Tsukanova, Anastasiia

    2015-01-01

    International audience; Articulatory synthesis could become a valuable tool to investigate links between articulatory gestures and acoustic cues. This papers presents the construction of an articulatory model of the velum which is intended to complete a model already comprising other articulators. The velum contour was de-lineated and extracted from a thousand of X-ray images corresponding to short sentences of French. A principal component analysis was applied in order to derive the main def...

  18. Titan II. Reliability and Aging Surveillance Program (RASP) Management Plan

    Science.gov (United States)

    1974-12-02

    following organizations: SAC OGDEN ALC * LGBT *MMER XPQM *MMCO BM MMCP V DEFS MMCR DOKM MMCT DOTM MMEW ~- V -~ -DOXX S • ,. _ X PQ T * Indicates...age and service on the Titan II Weapon System. This responsibility includes publication and dissemina- tion of reports and information. CINCSAC/ LGBT ...semiannually by the scheduling subcommittee composed of CINCSAC/ LGBT and Ogden ALC/ MMCO and MMER. LGBT will have primary responsibility for scheduling

  19. AcEST: BP914708 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4708 - Show BP914708 Clone id YMU001_000062_A06 Library YMU01 Length 87 Definition Adiantum capillus-veneris...ot (No blast op. Sequence too short.) sp_hit_id - Definition - Align length - Score (bit) - E-value - Report...inition - Align length - Score (bit) - E-value - Report - ... ... - TrEMBL (release 39.9) Link to BlastX Result : TrEMBL (No blast op. Sequence too short.) tr_hit_id - Def

  20. Navy Controls for Invoice, Receipt, Acceptance, and Property Transfer System Need Improvement

    Science.gov (United States)

    2016-02-25

    purpose of this guidance is to obtain reasonable assurance that the service provider’s controls are appropriately designed. DODIG-2016-054 │ 3...Department of the Nav y were designed and operating effectively. We also determined the effect of any identif ied def iciencies on audit readiness goals...it y. Wit hout correc t ing t hese CUECs it could impac t t he audit readiness goa ls of t he Nav y. Recommendations The Deput y Assistant